LOCUS       DASVMB010000001         5408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_662508,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000001 DASVMB010000000
VERSION     DASVMB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662508"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VF531_00005"
     CDS             <1..170
                     /locus_tag="VF531_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7712375.1"
                     /translation="SILVRQRGTKFHPGENVGIGKDDTLFAKIDGFVSFERKGKSDKQ
                     VSVYSEKKVTA"
     gene            383..1570
                     /locus_tag="VF531_00010"
     CDS             383..1570
                     /locus_tag="VF531_00010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7712376.1"
                     /translation="MKKYFRLWPVFYLALLGAVMLGQTAGAAGRVSGEANLKLDSYAA
                     ILIEPTSGEILFQKNQSEPLPPASVTKLMVMLLALEAVDKNQIKLTDVVTASPEACRM
                     GGSQIWLEPGEQMTVAELMKAVSIVSANDASYALAEHLAGSEENFITLMNKRCNELGL
                     KSTHYVNTTGLSPEDGGTGNITSVEDQARLAMEVIKHPLVFKWTGTWIDSLRGGKSFL
                     RNTNNLVRFFRGCDGLKTGFTNAAGFCLVATAKRDGVRLVAVVMKSPASETRSKDITK
                     LFNYGFSKFKAYQVFKGGETIGQVRVFRGRQAYVDAVVPGELTAIVKRDFQGKVIKRI
                     RLYPVIQAPVKTGQKIGAVELSVEGKNCGRIDLTAAKSVERASFFEICWQAFQNLIRV
                     GVR"
     gene            2051..2389
                     /gene="spoIIAA"
                     /locus_tag="VF531_00015"
     CDS             2051..2389
                     /gene="spoIIAA"
                     /locus_tag="VF531_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HEX7712377.1"
                     /translation="MNIETERIGANLIVKLEGELDLETSPLFRQVVDEQLNHYDSIRH
                     LILDLKQVRFIDSSGLGVILGRFRRLSQSGGRLSAINVSDPLRRLLQLSGLLKIMDIH
                     EDRRHALGEL"
     gene            2416..2871
                     /gene="spoIIAB"
                     /locus_tag="VF531_00020"
     CDS             2416..2871
                     /gene="spoIIAB"
                     /locus_tag="VF531_00020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HEX7712378.1"
                     /translation="MVKNYLKMEFPSLPQNVGQARLIVASFAAQLEFTLAEIEEIRVA
                     TSEAVSNCVIHAYSGQTGIIRLELEIEDDFLVIRVIDFGKGIEDIEQAKQATFSTEPE
                     RMGLGLVFMESFMDQMELKSSPSQGTMVVMKKRPERGFADAGVSRNSKE"
     gene            2840..3619
                     /locus_tag="VF531_00025"
     CDS             2840..3619
                     /locus_tag="VF531_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7712379.1"
                     /translation="MQGSPETVKSEINLPNTGLLTDQEFRELIMGYRNGDTEAKNVII
                     QHNLRLVMSIAQRFSNRGELDDLFQIGCMGLLKAVDKFDPTFEVKFSTYAVPVIIGEI
                     KQHLRDDGPIKVSRSMKELAGKVDQTRQQLGATLGKEPTLSQLESATGLSREEIAGAL
                     EVSRPLNSLQDIVYEEEGDAVSREMLIGEEVEHTKWLEHFALHEVIAKLPKRLRLLVE
                     LRFFEEKTQSEIAAIFGVSQVQICRLEKEALYRLRHLYLND"
     gene            3714..3905
                     /locus_tag="VF531_00030"
     CDS             3714..3905
                     /locus_tag="VF531_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712380.1"
                     /translation="MKPKGLFILLLVAFCIAASMIIYSAVQNSPEIPDKARLVQMDIS
                     PWNVLRVTSGYRFNVRCFT"
     gene            4179..4994
                     /gene="folP"
                     /locus_tag="VF531_00035"
     CDS             4179..4994
                     /gene="folP"
                     /locus_tag="VF531_00035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7712381.1"
                     /translation="MNSKTYVMGIINLTPDSFSSDGLYGRSDYIDRAVQQAKEMVDAG
                     ADFLDVGAESTRPGGPAVDEAEEARRLFPALRELVSAVKVPFSVDTYKPRIAEQVLNL
                     GVAMLNDIWGLLSPDDPERRMARIAGDSGVPVIVMHNAEKNAYQDLMPELIARLEDSI
                     RVALDHGVREEQIIVDPGVGFAKSQRDNLQVIRELDRLKILGRPILLGTSRKSVIGNA
                     LNLPVTERLEGTIATTVWGVIKGANIVRVHDVKAIVRASRMCDAIASAGDNQI"
     gene            5035..>5408
                     /gene="folK"
                     /locus_tag="VF531_00040"
     CDS             5035..>5408
                     /gene="folK"
                     /locus_tag="VF531_00040"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7712382.1"
                     /translation="MASLKQAAGLIAETVGVTPVTSAIYRSEPVEVTDQPWFYNQVLY
                     LVPPVAAEWSPSRLLQALKSIENQMGRVTSFRYGPRLIDLDLLFFRDWVFESSYLTVP
                     HPKVCERAFVLMPLVELDQKLV"
BASE COUNT         1326 a         1202 c         1480 g         1400 t
ORIGIN      
        1 gtagtatttt ggtacgccaa cgcggtacca agttccatcc cggcgaaaat gtcggaatcg
       61 gcaaggacga taccttattt gcgaagattg acggttttgt ttcctttgaa cgcaaaggca
      121 aatccgacaa gcaagtaagc gtctattccg agaagaaagt tactgcgtaa agggctgctt
      181 accctaagtt ctgttctcat tccccggtca attttaatga ccggggttat tttttgtccg
      241 ggaacgaccg attacccggc gggaacaggg aatagaaccc cccttttttg cacaagatca
      301 taaagcgatg gcgcatgcgt gctgtaatca tttccttatt tgaaagagtc agatcgcttt
      361 atcaatcaag ggggtttggt gcgtgaaaaa atatttcagg ttatggccgg tcttttatct
      421 tgcattactt ggtgcggtta tgctgggaca aacagccgga gccgcagggc gggtgagcgg
      481 tgaggccaat ttgaagctgg attcatatgc agcgattcta attgaaccga cgagcggtga
      541 gattcttttt caaaagaacc agtccgaacc gcttccaccc gccagtgtga ccaagttaat
      601 ggtgatgtta cttgcattag aggccgtcga caaaaatcag atcaaactca ccgacgtggt
      661 taccgcctct cccgaagctt gccggatggg cggttctcaa atctggctcg aacccgggga
      721 acaaatgacc gttgccgagt tgatgaaggc ggtgtcgatc gtttcggcca atgatgcctc
      781 ttatgctttg gcggaacatc tggcgggctc ggaagagaac tttatcacct tgatgaataa
      841 acgttgtaat gaattggggt tgaagagcac tcactatgtc aacactaccg gcttgagtcc
      901 cgaggacgga ggaaccggga atattacctc tgtggaagac caagcccggt tggccatgga
      961 agttatcaag catccactgg tttttaaatg gaccggaaca tggattgaca gtttacgggg
     1021 gggtaaatcg ttccttcgga ataccaataa tttagtaagg ttctttcgtg gttgtgacgg
     1081 gctcaagacc ggatttacca atgccgcggg tttttgcctg gtagcaaccg ccaaacggga
     1141 cggtgtgagg ttggttgccg tagttatgaa atccccggct tccgaaacac gttccaagga
     1201 cataaccaaa cttttcaatt acgggttctc taaattcaag gcttatcagg tttttaaagg
     1261 cggagaaacc attggccagg tccgggtctt tcgcggccga caggcatatg tggatgccgt
     1321 ggttcccggg gaactgacag ccatcgttaa gcgggacttt caagggaagg ttattaagcg
     1381 gatcaggctc tatccggtga ttcaggctcc ggtaaaaacc ggtcaaaaaa tcggcgcggt
     1441 cgaattgagt gtggaaggaa aaaattgcgg gcggattgac ttaacagccg caaaatctgt
     1501 tgaaagggcg tcgttctttg agatctgctg gcaagccttt caaaatttaa tcagggttgg
     1561 agtacggtag gcaagaggcg tgaggcatga ggcatgaata gcgcgcgccc cccgtggagc
     1621 gcgaggcaac tctcttctga aaagagaggg gttgggggtg agttgccagc gtgagatagg
     1681 aaataggcaa taacaacata tctcatctct tttgtctcct tcccgttcca agagttatgt
     1741 tgactggaca tacgacacgt ccgttggacg tgtgatatcc ggcttcaacc tttctttccc
     1801 cgtgacgggg aaagccagat aggggtcttc ccccaagtac tgttacgaaa taactaaagt
     1861 aaatattcaa agccccaggt ctagctgaaa ctcaagtaga cctggggctt ttaattttcg
     1921 tcatcaaatg atcaccttta tttaaaaagt gtcataagta ttgactttac ggccctttct
     1981 gtcattttct gagggaggat ctgaaagccg gacggcgaat tcttccccag ttcagaaagg
     2041 aggtttttgc ttgaatattg aaaccgaacg gatcggagcg aatcttatcg tgaaacttga
     2101 aggagagcta gacctggaga cctcgccgct ttttcgtcag gtcgttgatg agcagttgaa
     2161 tcattatgat tccatccgcc atctgatcct cgatttaaag caggtccgtt tcattgatag
     2221 ttcaggtttg ggggttattc tcggccggtt tcgacggtta agtcagtccg gggggagact
     2281 atcggctatt aacgtctcgg acccgttacg acggttgttg caactctcgg gtcttttgaa
     2341 gataatggat attcatgaag atcgccggca tgcattaggg gaattgtgat aaatctcatg
     2401 gaggtagcgg caagaatggt aaaaaactat ttaaagatgg agttcccgag tctacctcag
     2461 aatgtaggcc aagcccgatt aatcgtcgct tctttcgccg cccaactgga gtttactttg
     2521 gcggagatcg aggaaatcag ggtcgcaacc tcagaggcgg tttccaattg cgtgattcac
     2581 gcatattccg gacaaaccgg tatcatccgt ctggaattgg agatcgagga tgactttctg
     2641 gttatccggg taattgattt tggaaaagga attgaagata tcgagcaggc gaaacaagcc
     2701 actttttcga ctgagcctga acggatgggt ctgggtctgg tattcatgga atcatttatg
     2761 gatcagatgg agcttaaatc cagcccctcc caaggaacaa tggttgttat gaaaaagcgt
     2821 ccggaacggg ggttcgctga tgcaggggtc tcccgaaaca gtaaagagtg agatcaatct
     2881 ccccaatacc gggctcttaa ccgatcaaga gttccgggag ttgatcatgg gatatcggaa
     2941 tggtgataca gaagctaaaa acgtcatcat tcagcacaat ttacggttag tgatgagtat
     3001 tgcccaacgt ttctccaacc ggggcgaatt ggatgacttg tttcagatcg gctgtatggg
     3061 tctgttaaaa gcggtggata aatttgatcc cacctttgaa gtgaaatttt cgacctatgc
     3121 cgtaccggtg attatcggtg aaatcaagca acatctccgg gacgacggac ccatcaaagt
     3181 aagccggtcc atgaaagaat tggcgggtaa ggtggaccaa acccggcagc aacttggggc
     3241 gacactgggt aaggaaccta cactttctca actggaatcc gctaccggtc tttcccgtga
     3301 ggaaatcgcc ggggcgctgg aggtcagccg gccgctcaat tcattacagg atattgttta
     3361 tgaagaggag ggggatgcgg tcagccggga gatgcttatc ggagaagagg ttgagcatac
     3421 caaatggctg gaacatttcg cccttcacga agtcatcgcc aagcttccca aacgtttacg
     3481 tctcttggtc gagctccgct tctttgagga aaagacccag agtgagatcg ccgcaatctt
     3541 cggtgtctcc caagtgcaga tctgtcgtct tgaaaaagaa gcactgtatc gtttgcgtca
     3601 tctatacctc aacgattaag cgcgagcccg cgcggggttc aagctggaag gggtttggaa
     3661 agatctgtgg cataaaaatg attctatttt gagagaatag tttcggtgat ggcatgaaac
     3721 ccaaaggatt atttatcttg ttgttggtcg cgttttgtat cgccgccagt atgattattt
     3781 acagcgcggt gcaaaacagt ccggagatac cggataaagc ccggttagtt caaatggata
     3841 tctcaccttg gaatgtactt cgggtaacct cgggttatcg ttttaatgtt cgctgtttta
     3901 cataagagca taacttggta taaaaaacgc tttctgaaac gtggattgac tcgctgccac
     3961 tatcgcagcg ttttttctat ggcatcactt atttttgttt taagtctatt gcttgggtca
     4021 attcttcctc cgttcaggcc gggttgtgat ataataatac ggaaaaatta tcgttgcagg
     4081 ccaacccaat tggagggatt tccattcggc attccatttt gacgggtgca tcatcttctg
     4141 ttctgccgcc ggtttcaatc cagggcaaac cttatcactt gaattccaag acgtatgtga
     4201 tggggatcat caacctgact cccgattcat tttcaagcga cggtctttac ggccggagcg
     4261 attatattga ccgcgccgtg cagcaagcca aggagatggt ggatgcggga gccgattttt
     4321 tagatgtcgg cgcagagtcg acccggcccg gcggacctgc ggtagatgaa gccgaagagg
     4381 cgcgccggtt atttccggct ctccgggaac tggtttccgc cgtgaaggta cctttctcgg
     4441 tggataccta taaaccgcgc attgccgagc aagtcctgaa tctgggtgtg gccatgctca
     4501 atgatatctg ggggttgctt tctccggacg atccggagcg gaggatggcc cggattgccg
     4561 gagattcggg tgtcccggtg attgttatgc ataatgcgga aaaaaatgct taccaagatc
     4621 tgatgcctga actcattgca cgtttggagg attcgatccg ggtcgccctc gatcacgggg
     4681 ttcgtgaaga acagatcatc gttgatccgg gtgtcggctt tgccaaaagc caacgggata
     4741 atctccaggt catacgggaa ctggaccgcc tgaagatact agggcggcct atcttactcg
     4801 gcacttcacg caaatcagtg atcggcaacg ctttgaattt gcctgtcacc gaacggctgg
     4861 aggggacgat tgccaccaca gtctggggcg taatcaaggg tgccaatatt gtgagggtac
     4921 atgatgtcaa agccattgtt cgagccagcc ggatgtgcga cgccatcgca tccgccggcg
     4981 acaaccaaat ctaaccggat ctttatcggt ctgggcagca atctcgggga ttcggtggct
     5041 agcctaaaac aggcggcggg actcattgcg gagaccgtcg gggtaacccc ggttacttcg
     5101 gctatttacc ggagtgaacc ggtagaggtg acggatcaac cttggttcta taatcaagtg
     5161 ctgtatctgg ttccgcctgt ggcggcggaa tggagcccgt ccaggttgtt acaggcattg
     5221 aaatccatcg agaaccagat gggtcgggtt acgagtttca ggtatggccc gcgcttgatc
     5281 gatctggatc tgctgttctt tcgggactgg gtgtttgaaa gttcctatct gactgtgccc
     5341 catcccaagg tttgtgaacg ggcttttgtg ttaatgccgc tggtggaact ggaccagaaa
     5401 ctggttca
//
LOCUS       DASVMB010000002        18746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_294581,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000002 DASVMB010000000
VERSION     DASVMB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 18746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18746
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294581"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1540
                     /locus_tag="VF531_00045"
     CDS             431..1540
                     /locus_tag="VF531_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7712383.1"
                     /translation="MKVNRDIKIPLYTQVYESLIKMITEKKWQVGELLPSERELSIMF
                     NVDRLTVRRALGIIAQEGLVEKIAGLGTRVTGVSRHFEENPNFHNLIFLLPKIPKNLI
                     SADRITEPFNSSLFYSVENECKKQGYNLIYTTISDDELLTDILEGRGVQGILFVSKIN
                     EKFIEEAKKLKIPAVVVNNESDFYPTIRADREKGTYAAIKYLISEDHRQISFINGIPS
                     YITSKDCLHGYERALTDANIDWRRQIIKESDWTFDSGFKAMKEIIQENTQLPTAVFAC
                     NDTIAIGAMEAIKSAGFSIPQDISVIGFDDTEQSKYCSPKLTTVKVNIESMARIACQS
                     LFLEIKNREIQNIQIILPTELVIRESTAQAVHRTK"
     gene            complement(1599..2132)
                     /locus_tag="VF531_00050"
     CDS             complement(1599..2132)
                     /locus_tag="VF531_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712384.1"
                     /translation="MTDSLLLQMIDRRLKPKEWYEYSTPVLEGFRLIATRDDQKFAGL
                     PVSDEAGVTGSGRKPGFSPIKPYRDYFGRFTLTTRLVAEKLEVQFAIGYAWNNRVTTV
                     EDYANFSWLRLATHVAGIVYPNLALQKQLLEYLLGHSRKPRFVTTRVYHFLQNDLYQT
                     ITGLIREREKKAGIPEA"
     gene            complement(2213..3514)
                     /locus_tag="VF531_00055"
     CDS             complement(2213..3514)
                     /locus_tag="VF531_00055"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7712385.1"
                     /translation="MQDHHINIGILGLGTVGTGVARVLLEQRELLKTRSALTFNLAAI
                     AELNWDKKRDLDLGGVHCTTNVADILEDPAIDIVVETIGGYEPAFSLICQALRNRKSV
                     VTANKALVATKGRELFQIAAENGVDLLFEASVGGGIPIIKGLREGLVANRIESMYGIL
                     NGTSNYILTRMHRDDLEFEQALKEAQAKGFAEADPTLDIGGGDAAHKLTILASLASSG
                     FVDFSNIYVEGITGITKLDINFAKSFDYTVKLLAVYRLLDKGLDLRVHPTLVPNSHLL
                     AAVSNELNAIFVKGDFVGNTMFYGPGAGERPTASAIVSDIVDLSRDLLTSEGGEFHKQ
                     AIDPGRNVATVPIQEIRNRYYLRFFTLDAPGILSKISGALAEYGISISSMVQLETHGK
                     NNHVPIVLLTHEAREADMEQALAVIRKFDFVQENYLRLRLF"
     gene            complement(3923..4342)
                     /locus_tag="VF531_00060"
     CDS             complement(3923..4342)
                     /locus_tag="VF531_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7712386.1"
                     /translation="MKSFRKELLFHTKNRVEFINITEQVEAVLAESGIREGLCLVNAM
                     HITASVFINDHENGLWNDYRNWLEKLAPHEPSSLYQHNRTGEVNGDAHLKRQVMGREV
                     VVAVTAGRLDFGPWEQIFYGEFDGERPKRVLVKIIGE"
     gene            complement(4416..5048)
                     /locus_tag="VF531_00065"
     CDS             complement(4416..5048)
                     /locus_tag="VF531_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712387.1"
                     /translation="MSFSQDFNVKVGENTRRSDLAMPRFYIHYGRQDSGAVNRPAAYA
                     IISRLKGQSDLFLEIKSELLNLPPGNRGVAGVSFLETVQRLGLDYRHRKQTGPQNTRV
                     FGIEISLGKHTIEEHDILAYLPDSIWRREDFYSCLPLYGARYYVPASPLDASTLDASS
                     ILDQLWQGEIAPQTQLEVFRWIGFDCINFGQMGLLSNSLTLQDLKKTLEI"
     gene            complement(5139..5837)
                     /locus_tag="VF531_00070"
     CDS             complement(5139..5837)
                     /locus_tag="VF531_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712388.1"
                     /translation="MAVDKLSKLRMYAEVRFSFLDSTTHMEDLEGVVKELYRTEYITK
                     DELLLKESEPLLGPWEMRVKPQRIFFTMDSCPSYDYFGDVLENHLKTMAKVLTPKEII
                     NSGVQAYYLYPIDSLQDFSEVVTLWSDSYIQNKSSVVGISDLGVNVFFKEESLKVNIN
                     CKVLNDTQAKGYFPETGGKPVAQLNLLIDLDISTADPVELKKSLSPALVQILQNQINE
                     AVRQMEERLGNLVR"
     gene            complement(6006..7862)
                     /gene="guaA"
                     /locus_tag="VF531_00075"
     CDS             complement(6006..7862)
                     /gene="guaA"
                     /locus_tag="VF531_00075"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7712389.1"
                     /translation="MEIESIAILDCGGQYTKVIDRKVREMSIRSEIFPINVSAESLRG
                     YRGIILSGGPSSVWSDQALAYDPDIFKLGLPVLGICYGMQLLNQHFGGKVRPGTTTEY
                     GEIKLDVDTACPLFHGLEVTQRVLMSHGDSVTELAPGFKVAAKSGEIYAAIYDEGRRL
                     YGVQFHPEVDLTVQGRRMLENFLKAVCGLSGNYLLDDRIQTAIAKIREQVGDEKILVL
                     VSGGVDSAVSAALLLKALGPDNVYAIHIDHGLMRKNESDLVCENLKELGLRHLIRKDA
                     TDLFFNSVITVNSKELGPLTQVVDPEVKRNLIGEIFIRVVREVATELQLDFDRTFWAQ
                     GTLRPDLIESGNPDVSSFAHRIKTHHNDVDIVRRAREKGLVIETNWDWHKDEVRQVAR
                     SLGIAESIASRQPFPGPGLAIRVICHNGQGEITSTQRDALARVMAEKPTGIDAVAVPI
                     QSVGVQGDCRSYRNLAIAYGQGTGFDWETLYGIGKILPNRLDFINRVAYVLNRQQLSG
                     PLACHSLYINRESVALLREVDNLVVAKLNRKPISQVFAVLLPLGITKRYSVAIRTIIT
                     NDFMTGRAAVIGEDLPKELVLELVKELESRFPELDLILYDITGKPPATVEWQ"
     gene            complement(8146..9816)
                     /locus_tag="VF531_00080"
     CDS             complement(8146..9816)
                     /locus_tag="VF531_00080"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX7712390.1"
                     /translation="MLSDIEIANQSPLRPIREVAIRAGIDETFLEPYGKYKAKIDLGL
                     YRQLASQPDGKLIYVTAITPTPAGEGKTCTAVGLTQALGKLGKRVMLCLREPSLGPTF
                     GVKGGATGGGYSQVLPMDEINLHFTGDIHAVTTAHNLLAAMVDNHITQGNELGIEPKK
                     IVWRRVMDISDRQLRNIIVGLGGKTDGVTRETGFDITAASEIMAVLCLASNLEDLKER
                     LGRILVAYNGRNEPVYARDLKAVGAMAALLKDAVKPNLVQTLEGQPALIHGGPFANIA
                     HGNNSIIATRMALKLADYVVTEGGFATDLGAEKFFDIVSPVAGLKPDAAVLVVSARAL
                     KFHGGIEKLHLGEENLAALEKGFANLERHLRNLHSLYKLPVVVAVNRFPNDTPAELQA
                     ISDYCTQLGVPATVSEVVARGGEGGVELAGAVLETLKQPNGFKPLYDHELPLRAKIET
                     IATQVYGAARVNYSKEAETALNRLTELGYARLSVCIAKTQMSFSDDPSLKGAPSGWTL
                     TIREIRLLAGAGFVVAIAGTIMTMPGLPKHPSAENVDITGDGEIKGLF"
     gene            10073..10846
                     /locus_tag="VF531_00085"
     CDS             10073..10846
                     /locus_tag="VF531_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX7712391.1"
                     /translation="MEQARRKPAFLNFDGSYLQQKHLLAEPHLWLDFSDLVQSGMFCT
                     PETGRIIKQRLEQNEDSDIHFIGSGNYHYVTLFFLAKIKHPFSLVLFDNHSDANDTGH
                     CLSCGSWVAEAISRLKELKQVVLIGADPQCLGLIHPQLLNKLIFLTEESLQKTPLPEI
                     VAGIPTGLIYISIDKDVLDPGYAVTQWDQGRMSLPELLTRLQILLRLKKVTGIDVCGE
                     YPASPVELFQSSVQQAIRKNEYANEQILSLVVRNVGNRQ"
     gene            complement(10913..11761)
                     /locus_tag="VF531_00090"
     CDS             complement(10913..11761)
                     /locus_tag="VF531_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7712392.1"
                     /translation="MIFNFSSVSETLLIPLYCRAMETESREPVIKDAKAVAIMRRLDQ
                     ELATSDRKLAQALVRRRLPKKLVVTMSLRTRRFDEYVRSFVGTHEHPVVVTMGCGLDT
                     RASRIDDERIDWYDLDLPEVIAVRQKFFADDDRHHSIGCSVLDFRWMEQLPKAQAEFL
                     FVAEGLFMYLPESEVQKLLLQLQARFPGSELACEVSNRYWVQKMQSGHYRRKFQKQLY
                     LDEGAVFRFGISNGKELETWNEGIDLLDEWTYFDEHEKKLGWYNWFGQIELLRKVQWI
                     AHYKLG"
     gene            complement(11843..13066)
                     /locus_tag="VF531_00095"
     CDS             complement(11843..13066)
                     /locus_tag="VF531_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7712393.1"
                     /translation="MKPIILTQLQARQFILAYQGLQPSLELTGKSGVLEYIRRVGCIQ
                     YDPLDIVGHNPELVLQARVAGFHPAMLQELLYSDRKLLDGWDKMMAVYPVEDWPYFRR
                     QREAAREYFGNSSRPVVAVLPQVRQAIEAQGPLSSIDLQLNEIVDWPWAPTRLSRAAL
                     ESMYFWGELVIHHKVHTRKVYDFARRHLPAELLEAADPNITAEQYQDWRVLRRIGGIG
                     LVWAKSGDAWLGTEIKSKERAASLARLLKTGKIIEVRITDLETPFYMRSADRLYLERV
                     LNSGDPAPHAAILAPLDNLLWDRRLIKTLFDFDYRWEVYKPVTERQYGYYVLPVLYGD
                     GFVARFEPGRDKKGKALMIKNWWWEPGVKRRQKMDRALGQCFQNFLNYLGAERIQMDK
                     KVLAQADLEFLISKN"
     gene            complement(13465..15939)
                     /locus_tag="VF531_00100"
     CDS             complement(13465..15939)
                     /locus_tag="VF531_00100"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7712394.1"
                     /translation="MKQRILKRKLISAELKKIFDYRLTVVVAAMGYGKTTSVKTFLYE
                     AEADYVWLSVDSDEASPQYIWDCLTRQLAKVKPDLGDRLNALGFPIDAPQRDRVVGAI
                     VEYAFSTNTVLVIDDYHLARSPELDRLLERIVRSDISGLHIVLISRTKPELNTEELQL
                     KGYCCLFESDLFELSKQEIKEYFKLFGKDISDDTSGQVHGISEGWITAVYLMIQRYCE
                     TGKLEMGKNIEVLIEKAIMSRYTPEEAKLLIALCILNYFTPEQAVYVTGNPAAAGTIQ
                     RLCLDNSLIRYDQRTETYKIHHIFSGYLQKLLKERFSGTELQKLFRRAGEWHIQNGDV
                     LSGLMAFLKAKEYDLILTEFEKPGITKIVDRYPQSIVKLFEQIPPAVKYRHPLGYLLY
                     ADFYLSNIDMEDGTELLAQIEKYYRDDLATPPDLKNRIFGEIELSRTFVYFNDLRKMH
                     ECQLKAYQLLDGGSSIANKDMIFTFGSPHLLYLYYRDRGELRWIADYADQVFHYYSEV
                     SHGCGTGFEYLVNAEYYLETGDFAEAETYAQKTIYKAQTMAQVSIIICANLTLARLYA
                     AQGRFAEVVELINDLYAGVDEENSPVLYGAVDLLSGYVGAVLGEPGLIPGWLKTGDME
                     QSRILYQGLGFNYIVHAKALLLEGNYLKLEVLCEEMQQIFTQFNNLFGFLHVHIMNAA
                     VQYKLYGMEKAQNALETALEIGRADGIILPFAEYGVYILDILKALKNDSGNDTYLDKL
                     IKEASRYCSNLELLAGEKTAAGELTEREREILRLVGEGQSNREIAAGLYLAEITVKKN
                     ISSIYRKLGVEGRAAAVKKALELKLI"
     gene            16189..16550
                     /locus_tag="VF531_00105"
                     /pseudo
     CDS             16189..16550
                     /locus_tag="VF531_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003327893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            16695..17177
                     /locus_tag="VF531_00110"
     CDS             16695..17177
                     /locus_tag="VF531_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7712395.1"
                     /translation="MIFPVIMYVLAILLLIISFTKDAPKTILALKKALNIFVGLLPQF
                     IAILLLVGMILAFLNPKTIEAIIGSQSGLPGLILSALTGSFTIMPVLIAFPIAVELLK
                     NGAGLMQMAVFISTLTTVGFMTIPLEAKYLGMKVALFRNCVFFIFSFISAWIIGMILS
                     "
     gene            17174..17701
                     /locus_tag="VF531_00115"
     CDS             17174..17701
                     /locus_tag="VF531_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7712396.1"
                     /translation="MKKNLLHDPFLITVLGITLLLLIFNPRIGEKSLALSWNNLPSII
                     FMLTPIFIFMGLLDVWVPKETMIRIMGEHSKGRGALIALLLGAITAVPLYGLLPMTGM
                     LLKKGCKLSNILIFLCSSSSLRIPLFLFEVSSLGWQFTLIQFLTSLIAVLVIALLVEK
                     ILTEKERTDIYNRAQ"
     gene            complement(17754..>18746)
                     /locus_tag="VF531_00120"
     CDS             complement(17754..>18746)
                     /locus_tag="VF531_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712397.1"
                     /translation="LVKHAESRSFDTENGVYHTYFYHRVSKWRECNQALGSTVKPEGP
                     IPVWPEAFEQYRLPHFLEGQVHALRCMEDKDRSQDLYRAVRESALFDRELGMYKVCAD
                     LSGAPQELGRIRVFPRGWLENESIFLHMEYKYLLELLRSGQTSEFFAALPEVLVCFAD
                     PAKYGRNPLENCSFIVSSAYPRAEMHCNGFVARLSGSTAEMLNLWLWMSFGPRPFRLN
                     DAGDLELIFEPILDSRFFSHSAAEVEINGTEGAISAQLSADNYAAIFLGHTLVVYQNP
                     SLRPTFGPDAAKVRNCLLYFRDGQKLEITGPRISGEPARQVRAGLVERIELYLE"
BASE COUNT         4768 a         4932 c         4350 g         4696 t
ORIGIN      
        1 cgctgataaa ggggctacga tttactggga atagttgtac acgtctgtat gaccggttct
       61 gtaccgactc caggggtaca gccatgtctt tatgttggtg ctttccggaa gtggtcgcat
      121 aacccccgga tacagactta tctagtattg agccattatc aaatcttcac ttatgaatgt
      181 cgtccataac aaactcattc cagccgggga ttttgatttt ggctcaattt ttaaaaccct
      241 ttccagccaa acctgcttcg aggatttgac cgaagaagag tttaatcgtt ccgataccaa
      301 atctccacgt cggcggaaaa atcgttgaaa tcattgatta aaggcggata atctggtatc
      361 cttatccatg aaataattca acaggcaaag attaccaaag taaataagta cttgtgagtg
      421 agagaaaagc atgaaagtta accgagatat taagataccc ttgtatacgc aggtatacga
      481 gagtttaatc aaaatgatta ccgaaaagaa atggcaggtt ggggaactct tgccttcgga
      541 acgcgaattg agtatcatgt ttaatgtcga tcggctgacg gtccgcagag ctttggggat
      601 aatcgctcag gaaggtctgg tcgaaaagat tgccggactg ggaacccggg taacgggtgt
      661 atcacgtcat ttcgaggaga accccaactt tcacaatctg atatttttgt tgcctaaaat
      721 tccaaaaaat ctaatctcag cggatcgaat caccgaacca tttaactcca gtctgtttta
      781 cagcgttgaa aacgaatgca aaaaacaggg atataattta atatacacta ccataagtga
      841 cgacgagctt ttaaccgaca ttctggaagg ccgaggggtt caaggtattt tgtttgtaag
      901 taaaatcaat gaaaaattca tcgaagaagc caaaaagctg aaaattcccg cggtagtggt
      961 caataacgaa tccgattttt accctacaat ccgggctgac cgtgaaaaag gaacctatgc
     1021 ggcgataaag tacctgatat ccgaagatca ccggcagatt agctttatca acggaatacc
     1081 cagttatatt acttcaaaag actgcctgca tggatatgaa agggctttga cagatgcaaa
     1141 tatcgattgg agacggcaaa tcatcaagga aagcgattgg accttcgata gcggcttcaa
     1201 agccatgaag gaaataatcc aggaaaacac ccagcttccc accgctgttt tcgcctgtaa
     1261 cgatacgatt gccatcgggg caatggaggc aataaaatcc gccggttttt caattcccca
     1321 agatattagt gtgattgggt ttgatgatac cgagcaaagt aaatactgta gccccaagtt
     1381 gaccacggtc aaagtaaata tcgaatcaat ggccagaatt gcctgccaaa gcttattttt
     1441 ggagattaaa aaccgggaga ttcaaaatat ccaaatcatt ctcccaaccg aactcgtcat
     1501 tcgggaatcg actgcccaag cggttcatcg aaccaaatag ctaaatatga tatcaaccat
     1561 agagtccccc accttcaata gggggttagg ttcgcttttt aggcctccgg tataccggcc
     1621 ttcttctccc gctcccggat caacccggtg atggtctgat aaaggtcgtt ctggagaaaa
     1681 tggtagaccc gggtcgtaac aaaccgtggt ttccgggaat ggccgagcag atactcgagg
     1741 agttgttttt ggagggcgag attggggtag acgatccccg cgacatgagt ggccagacgc
     1801 aaccaactga aattagcgta atcttccacc gtggtcaccc ggttattcca ggcataaccg
     1861 atggcgaatt gcacctctag cttttccgca accaaccggg ttgttaaggt gaaacgaccg
     1921 aaataatcgc gataaggttt gatcgggcta aatccgggct tgcgtccgga cccagttacg
     1981 ccggcctcgt cgcttaccgg aagtccggcg aatttctgat catccctggt ggcgatcaac
     2041 cggaaccctt ccaataccgg agtggaatat tcgtaccact ctttcggttt gagcctgcgg
     2101 tcgatcatct gcaacaatag actatcggtc atagatagcg acacccgctt ccggttcttc
     2161 tttttccatg cccaccctcc ctttgcgccc tttgcgggtg ccggggtaag ggttaaaaca
     2221 gtcgcaaccg caaatagttc tcctgcacaa agtcgaactt gcggatcacc gccaaggcct
     2281 gttccatatc ggcctcgcgg gcctcatggg ttaggagcac aatcggtaca tgattgtttt
     2341 tgccgtgagt ctccagttga accatggagg agatggagat cccgtattcc gccaaagccc
     2401 ccgagatctt ggagagaatg cccggcgcat ccagtgtaaa aaacctcagg taataacggt
     2461 tgcggatctc ttgaatcggc accgtggcaa cgttcctgcc gggatcgatg gcctgcttgt
     2521 gaaactcccc gccctcgctc gtcaagagat cacgggagag atcgacgata tcgctgacaa
     2581 tcgcgctggc ggtaggccgt tcaccggctc cgggcccgta aaacatggta ttgcccacga
     2641 aatccccctt gacgaagatg gcgttcaact cgttactgac tgctgccaaa aggtgcgagt
     2701 tgggcaccag cgtcggatgg acccggaggt ccagaccttt gtcaagcagt ctgtagactg
     2761 ccaatagttt gacggtataa tcaaaactct tggcaaaatt gatatccaat ttggtgatcc
     2821 cggtaatgcc ctcgacgtaa atattgctga aatctacaaa gcccgaggat gccagcgacg
     2881 ccagaatggt aagcttatga gccgcgtcgc cgccgccgat gtcgagggtg ggatcggctt
     2941 cggcaaatcc cttggcctga gcctctttca gggcttgctc gaactccaga tcatcgcggt
     3001 gcatccgggt cagaatatag ttggaagttc cgttaaggat cccgtacatg ctttcaatcc
     3061 ggttggcaac caatccttca cgcaaaccct tgattatggg gatgcctccg ccgacgctcg
     3121 cttcaaacag gagatccact ccgttctcgg cggcaatttg aaagagttcc cgccccttcg
     3181 tcgccaccag tgctttattg gccgtgacca cgcttttcct attgcgcagc gcctgacaga
     3241 tcaggctgaa ggccggctcg taaccgccga tggtctccac tacgatgtcg atggccggat
     3301 cctccaatat atccgccaca ttcgtggtac aatgaacccc gccgagatcg agatcacgtt
     3361 tcttgtccca gttcaactcg gcgattgccg ccaaattgaa ggtcaaagcg gagcgggttt
     3421 tcaaaagttc ccgttgttcc agtaatactc tggccactcc ggtaccgacc gtccccagtc
     3481 ccagtatacc gatgttaata tggtgatcct gcatgtcaaa acctcccaat cataatgagc
     3541 aatgtcaccc ttaccgccta tgggatcaaa aaagcctttc atcctctatt caaaggacga
     3601 aaggcttatc ttccgcggta ccaccttttt tgacctgccc catgggggcg ggcccaactc
     3661 atgccggata acggccggac cgggccacca tattcaagcg ccgtacagtg cgctttttcc
     3721 ggaagccgct caggggtggt attgccgatt ttgccgggaa ggctttcagc cgcggccctc
     3781 cctctctata tcccgggcgc cggaaaaatt cccggtaccg cccaacccgt caacatgtcc
     3841 ccgtcatcgc tttacgtttt ttatactata ccaccgccaa gatggaaagt caatccgatt
     3901 atggttgaaa tttgggtgat atctattcgc cgatgatctt gacgagtacc cgtttaggcc
     3961 gttcaccgtc gaattccccg taaaaaatct gttcccaggg gccgaaatca agccggccgg
     4021 cggtgaccgc caccactact tcacgcccca ttacctgccg ctttagatgg gcgtcgccat
     4081 tgacttctcc ggtccggtta tgctgatata aggaactggg ttcatgggga gccaatttct
     4141 ccagccaatt ccggtaatcg ttccagagcc cgttttcatg gtcattaatg aagacgctgg
     4201 ccgtaatatg catcgcgttg accaggcata gcccctcccg gatgccgctc tccgccagga
     4261 cagcctccac ctgttcggtg atattgatga attcgacacg gttcttggta tgaaagagca
     4321 actctttacg gaaggatttc atttaaaacc tccggttaaa aaagggtgaa gagtgaaaca
     4381 aaacccaaag acttcaggga tttccatgag aatagctaaa tctcaagtgt ttttttcaaa
     4441 tcctgaaggg ttaaggaatt cgagagaagg cccatctgtc cgaagtttat acagtcgaaa
     4501 ccgatccagc ggaagacttc caattgggtt tgcggagcaa tctctccctg ccagagttga
     4561 tcgaggatgc tcgacgcgtc gagagtcgat gcatcgagag gtgatgcggg aacataataa
     4621 cgggcgccgt aaagcggcag gcaggagtaa aaatcctctc gccgccagat tgaatccgga
     4681 agataagcca gaatatcatg ctcctcaatc gtgtgcttgc ctaaagaaat ttcaatcccg
     4741 aaaacccggg tattttgggg acccgtctgt ttacgatggc gataatcgag tccaagccgt
     4801 tggacggttt ccaagaagga tacccccgct acgccccggt tacccggagg cagatttaga
     4861 agctcgctct tgatctccag gaacagatcc gactgccctt tgaggcggga gatgatcgcg
     4921 taagccgccg gacggttgac tgccccgctg tcctgcctgc cgtaatgaat ataaaaacgg
     4981 ggcatggcaa gatctgaacg gcgggtattt tcgcctacct taacattaaa atcctgagaa
     5041 aacgacaatt gagaccctca tttcttttgc cttgattctc atgattacca tacaaaacct
     5101 gacttttgcc caaagagggg gttaagaagg gaattctatt accggaccaa attacccaat
     5161 cgttcctcca tctgccggac cgcttcattg atctgattct gaagtatctg taccagcgcg
     5221 ggagagagcg attttttcaa ttcgaccggg tcggcggtgg agatatcgag gtcgatcaat
     5281 agatttaact gggccaccgg ttttcctccg gtttcgggga aataaccctt ggcctgagta
     5341 tcgttgagaa ctttacaatt gatattaact ttgagactct cttctttaaa aaaaacatta
     5401 acgcccagat cgctgatacc caccactgag gatttattct gaatataact gtcactccat
     5461 agggtaacca cttccgaaaa atcctgcagc gaatcgatgg ggtataaata ataggcctga
     5521 accccgctgt taatgatctc ctttggggta agcaccttgg ccatggtttt cagatggttc
     5581 tccaacacat caccaaaata atcataactc ggacaggagt ccatggtgaa gaagattctc
     5641 tggggtttaa cccgcatttc ccaggggccg agcagcggtt cactctcctt caggagcagt
     5701 tcgtctttgg taatatactc ggtccggtat agctccttga cgacgccttc cagatcctcc
     5761 atatgggtcg tggagtccag aaaagagaat cgcacctcgg catacatgcg taacttcgaa
     5821 agtttgtcaa cggccatttt agttctatca cttccccgga caagatggaa ttccggattt
     5881 gttgtcgata cccgtacgac aaaacaaaga atttactaag ttgcgagatg ctaatcttct
     5941 tcattcatag ttattattcg acacagaagc agtaagtcct gcgttgttga tcgatcaatc
     6001 ggatcttatt gccattccac tgtggccggg ggttttccgg tgatgtcgta aagaatcaaa
     6061 tccagctccg gaaagcggga ttcaagttcc ttgaccaatt caagcaccag ttccttggga
     6121 agatcttccc cgatcacggc agcccttccg gtcatgaaat cattggtgat gatggtacgg
     6181 atggccacgg aataccgctt ggtgatgccg agtggaagta agaccgcaaa cacctggcta
     6241 atcggtttac gattcaattt cgccactacc aggttgtcca cctcccggag aagcgccacg
     6301 ctttcccggt tgatataaag cgaatgacag gcgagcgggc ctgataactg ctgccggttc
     6361 agcacatagg caacccggtt aataaaatcc aaccggttgg gaaggatttt accgatcccg
     6421 taaagggtct cccagtcaaa accggttcct tgtccgtatg caatcgccag attacggtag
     6481 ctacggcagt ctccctgaac cccgacggat tgaatcggca ccgccacggc atcgatcccg
     6541 gtcggtttct ccgccatcac ccgagctaag gcgtcccttt gggtagatgt gatctctccc
     6601 tgaccattat ggcagattac ccggatggcc aatcccggtc cgggaaaagg ctgacgtgag
     6661 gcaatgcttt cggcgattcc caagctccgg gccacctgtc gcacctcatc tttgtgccaa
     6721 tcccagttgg tctcgattac caatcctttc tcacgagcac gccggacgat gtcgacatcg
     6781 ttatgatggg ttttgatccg gtgtgcaaaa ctactgacat ccggattgcc gctctcgatc
     6841 agatccggac gaagagtgcc ttgcgcccaa aaagtccgat caaagtcgag ctgaagctcg
     6901 gtggccacct cgcgtaccac ccggatgaag atctcgccaa tcaggttcct tttgacctcg
     6961 ggatcaacca cttgggtcag ggggcccagt tccttgctgt ttactgtaat cacgctatta
     7021 aagaagagat cggtggcgtc tttccgaatt aggtggcgca gtcccagctc ttttaagttt
     7081 tcacagacca gatcgctctc gttcttgcgc atcaggccgt gatcgatatg gatcgcatag
     7141 acgttatccg gtcctaaggc ttttagaagt agcgccgcgc taacggcgga atcaacccct
     7201 ccgctgacca ggaccaggat cttctcatcc ccgacctgct cgcgaatctt ggcaatcgcg
     7261 gtctgaatcc ggtcatccaa aagataattg ccgctcagac cgcaaactgc ctttaaaaag
     7321 ttctccaaca tccgccggcc ttgaacggtg agatcgactt ccggatgaaa ctggaccccg
     7381 tatagacggc gtccctcatc gtagatcgcc gcgtagattt cgccggattt ggcggccacc
     7441 ttgaaaccgg gggctaattc ggtaaccgaa tcaccgtggc tcatcaggac tcgttgcgtt
     7501 acttcaaggc catggaaaag cgggcaggcc gtatcgacat ccaacttgat ttcgccgtac
     7561 tcggtggtcg ttcccggacg tactttgccg ccgaagtgct gattgagcag ttgcataccg
     7621 taacagatcc ccagcaccgg taagcctagc ttaaatatgt cgggatcata ggccagggcc
     7681 tgatcgctcc atacgctgct tgggccaccc gaaaggatga ttccgcgata tccccgtaaa
     7741 ctttcggccg aaacattgat gggaaagatc tccgaccgaa tcgacatctc ccgtactttg
     7801 cgatcgatca ccttggtata ctgaccgccg caatcgagaa tggcaatgga ctcaatttcc
     7861 aataagatca ctccgttatt ttaataggca ataggcacta ggcaacaggc aacagtagtt
     7921 tggtgaaaac ctgaaaattt catcgacagg caagcatact gcataaaagg caacagtagt
     7981 ttggtgaaaa cctgaaagtt tcatcgacag gcaagcgtac tgcataaaag gcttgcgtct
     8041 atacatacag aattggatca taggtttgaa atccatatcc cacagttttt atgcctcacg
     8101 cctatcgcct gttgcctttt ccctgttgcc cattgcctgt tcccttcaga acaaaccctt
     8161 tatctcccca tcccccgtaa tgtccacgtt ctcggcggag gggtgcttgg gcagtcccgg
     8221 catggtcatt atcgtgccag cgatggcaac cacaaaaccc gccccggcca gcagccggat
     8281 ttcccgtatt gtcagggtcc aaccactggg cgcgccttta agggaagggt catcagagaa
     8341 ggacatttgc gttttggcga tgcagaccga aagcctggca tatcccagtt cagtgaggcg
     8401 attcaaggca gtctccgctt ccttggaata attaacccgg gcggcgccat agacttgggt
     8461 ggcgatggtc tcgatcttgg cccggagggg taattcgtga tcgtaaagcg gcttaaaacc
     8521 gtttggctgt tttagcgtct caagcactgc tccggccagt tccacaccac cttcgccgcc
     8581 tctcgccaca acctcagaaa cggtcgccgg aacccctagt tgagtgcaat aatcgctaat
     8641 agcctgtaat tcggcgggag tgtcattggg aaaacggttc accgccacga ccaccggtag
     8701 tttatataga gaatgtagat tccggagatg gcgttccaga ttggcaaagc ctttctccag
     8761 cgccgcaagg ttttcctcac ccagatgaag tttttcaatt ccaccgtgaa atttcaacgc
     8821 tcgcgccgat accaccagta ctgccgcgtc aggcttcaaa cccgcaaccg ggctgacgat
     8881 gtcgaaaaac ttctcggccc cgagatccgt ggcgaatccg ccctcggtaa cgacataatc
     8941 agcgagtttc aacgccatcc gggtagcaat gatggagtta ttgccatggg cgatattggc
     9001 gaaagggccg ccgtggataa gcgcgggttg tccttccagg gtctgaacca gattgggttt
     9061 gacggcatct ttcaaaagtg ccgccatcgc cccgacagct ttcaaatccc gggcatagac
     9121 cggttcgttg cgcccgttat aggccactaa aatccggcct agccgctcct taaggtcctc
     9181 gagattcgag gctaaacaga ggacagccat gatctccgaa gccgcagtga tgtcgaagcc
     9241 ggtctcgcgg gtgacaccgt cggttttccc acccaatccc actatgatgt tgcggagctg
     9301 gcgatcggag atgtccatca cccggcgcca gacaatcttc ttgggttcta tccccagctc
     9361 attaccttgg gtgatatgat tatccaccat cgccgccaga agattatgag ccgtggttac
     9421 cgcatgaata tccccggtaa aatgaagatt gatctcgtcc ataggtagaa cttgagagta
     9481 tccgccgccc gtggctccgc ccttcacccc aaaagtcggt cccagagagg gctcccgcaa
     9541 gcaaagcatg acccgcttgc ccaatttacc gagggcttgg gtcaagccca ccgcggtgca
     9601 tgtcttcccc tcaccggccg gagtgggagt gatggcggtc acgtagatca attttccatc
     9661 gggctgactc gccaattgtc gatataggcc caggtcaatt ttggctttat acttgccgta
     9721 aggctctaga aaagtctcat cgatccctgc ccggatcgca acctcccgga tcgggcgcaa
     9781 cgggctttgg tttgcaattt caatatcgga aagcatccga gtcctcctgg ataatgacac
     9841 tcacgctaaa tattattata ttatatcaga ttaagcgcaa tgaagacaat atccataaat
     9901 taagccggcg gcgtcccggt cgaaggttcc ccatctctat cccgcttgat ttccaatgtg
     9961 atataatgat cactgccaat ccggacaacg ggagcattga aggaaggctg cccgggcgga
    10021 tggttcccat ccgtacctga atggttcatt tcggtcaaaa ggaggggtaa acatggaaca
    10081 agcccgccgg aaaccggcat tcctcaattt tgacggcagt tatctccagc aaaagcattt
    10141 gttggctgaa ccccacctgt ggctggactt ttcggatctg gttcaatccg ggatgttctg
    10201 cactccggag accggacgga taattaagca acggcttgag caaaacgagg attcggatat
    10261 tcactttatc ggcagcggta attaccatta tgtgacattg tttttcctgg cgaaaatcaa
    10321 acatccgttt tcattggtac ttttcgataa tcactccgat gccaacgata ccgggcactg
    10381 cctctcttgc ggctcctggg tggccgaggc catctcccgg cttaaagaac taaagcaggt
    10441 cgtgctgatc ggcgccgacc cgcaatgttt ggggttgatc cacccccaac tcctgaataa
    10501 actgattttt ttaactgagg agtcgctgca aaaaacgccg ctcccggaaa ttgtggccgg
    10561 gattcctacc ggcttgattt atatcagtat tgacaaggac gtcctggatc cgggctatgc
    10621 cgttacccag tgggatcaag gccggatgag cctcccggaa ctcctgaccc gcctccaaat
    10681 cctactccgc cttaaaaaag taactggcat tgatgtctgc ggcgaatatc ccgcaagccc
    10741 tgtcgaactt ttccaaagca gcgtgcagca ggcaatacga aagaacgagt acgcgaatga
    10801 gcagatctta agtctggtag taagaaatgt gggcaacagg caatagacaa caggcaacag
    10861 gaatttcttt cgtgtcccct gttgcctatt gcctagcccc tattgcctat attcatccca
    10921 atttataatg cgctatccac tggacctttc gaagcagctc gatctggccg aaccaattat
    10981 accacccgag ttttttctcg tgttcgtcaa agtaagtcca ctcatccaga agatcaatgc
    11041 cttcgttcca ggtttccagc tctttgccgt tcgagatgcc gaaccggaat acggctccct
    11101 cgtccaggta aagctgtttt tgaaatttac gccgataatg gccggattgc atcttttgaa
    11161 cccaatagcg gttggatacc tcacaggcca attcactccc aggaaaacga gcctgcaatt
    11221 gaaggaggag cttttgaacc tcgctctccg gaagatacat gaataatcct tccgcaacaa
    11281 ataggaattc agcttgggct ttcggcagtt gctccatcca acgaaaatcc aggactgagc
    11341 agccaattga atgatgacgg tcatcatcgg caaagaattt ctggcgaacg gcgattacct
    11401 cgggtaaatc caggtcgtac caatcgatcc gttcgtcatc gatccgggaa gcccgggtat
    11461 cgagcccaca tcccatggta acgacgaccg gatgctcatg ggtaccgaca aacgaacgga
    11521 catattcatc aaacctccgg gtacgcaggg acatcgtgac taccagtttt ttcggaagcc
    11581 ggcgccggac caatgcctgg gccaacttgc gatcggaggt tgccaattct tggtcgagcc
    11641 ggcgcatgat cgctacggcc ttcgcatcct tgatcactgg ttcccgggat tccgtttcca
    11701 tggcccggca gtataatgga atcaaaagcg tctccgaaac cgacgaaaaa ttaaatatca
    11761 tcagcttacc ccccttttta tactcagccc gatgtatttt ttcatagatt tcacataatg
    11821 tcccaatgcc ggctacagcc aatcagtttt ttgaaatcaa gaactctaaa tccgcctggg
    11881 ccaacacctt cttatccatt tggatccgct ccgcgcccaa atagttcagg aagttttgaa
    11941 aacactggcc caatgcgcga tccatctttt gccggcgctt gactcccggt tcccaccacc
    12001 agtttttgat catcagggcc ttgccttttt tatcccttcc cggttcgaac cgggctacga
    12061 aaccatcgcc gtataggacc ggaagcacgt aatacccgta ctgacgctca gtgacaggtt
    12121 tataaacctc ccaccggtag tcgaagtcaa acagcgtttt tattaacctc cgatcccaga
    12181 gcagattatc gagtggagcc aaaatggccg catggggagc cggatcgccg gaatttaaaa
    12241 ctcgttccag ataaagccga tccgcgctac gcatataaaa cggggtttcc aaatcagtga
    12301 tccgcacttc gataattttg cctgttttca ataagcgtgc cagggatgcg gcgcgctcct
    12361 tactcttaat ctctgttccc aaccaggcat cacccgattt tgcccaaacg agtccaatcc
    12421 cgccgattcg gcgcagtacc cgccaatcct gatactgttc cgcggtgata ttgggatccg
    12481 ccgcctccaa aagctccgcc gggagatggc gccgggcgaa gtcgtacact ttgcgggtgt
    12541 gcaccttatg gtggattacc agttcacccc agaaatacat gctttccagt gccgccctgg
    12601 ataatcgagt cggcgcccac ggccaatcta cgatctcatt gagttgcagg tcaatcgagg
    12661 agagcgggcc ttgcgcctca atggcctgac ggacttgcgg cagaaccgcc acgaccggac
    12721 gggagctgtt accgaaatat tcccgggcgg cctcacgttg gcggcgaaaa tacggccaat
    12781 cctccaccgg ataaacggcc atcatcttgt cccagccatc cagaagctta cggtcactgt
    12841 aaagcaactc ctggagcatt gccggatgaa accccgccac ccgggcctga agcaccagtt
    12901 ccggattgtg acccacgata tccaggggat catattgaat acaacccact ctgcggatat
    12961 actcaagcac gcccgatttc ccggtcagtt cgagagacgg ctggagtccc tgataggcca
    13021 gaataaattg acgggcttgc agttgggtaa gaattatcgg tttcatcacg ggttactcca
    13081 ttgaacagtc aagtattttc agaataacac aacttctttt atcatatcaa acatgcgttc
    13141 gaaaatcaat tcctatccag aattataacg ctaaatctgc gcggacgggg tcaatggctg
    13201 ccgtactccg gcataaaaaa agtacagggc cccctaaagg acgcctggac tttttgataa
    13261 aaggcttttt ctgctttgcc gggaacaatt ccggttaaaa gacttccttt ttacacggga
    13321 gccttctctt gcccccaaga gaaggtaacc catatgcttc gctgccaatt cttcttcgac
    13381 actgcctttt ccctttctct tgcgctctcg caagagcgct ctcggaaaag aaggagccag
    13441 gggttgagtt ccattcaaac cggctcagat cagtttcaac tccagcgcct ttttcacggc
    13501 cgccgcccgt ccttcaaccc ccaatttcct gtagatggag ctgatatttt ttttgaccgt
    13561 aatctccgcc aaatacagcc cggcggcgat ttcccggttg gattggccct cccccaccag
    13621 ccgcagtatt tcccgttcac gttcggtcag ctctcccgca gccgttttct caccggcaag
    13681 aagctccagg tttgagcaat aacgggatgc ttcctttatc agcttgtcca gataggtgtc
    13741 atttccggaa tcatttttca gagctttcaa aatatcaagg atataaaccc cgtattcggc
    13801 aaagggcaga attatcccgt cggccctgcc gatctctaag gccgtttcca gcgcgttttg
    13861 ggctttttcc atcccgtaga gcttgtattg gactgcggca ttcatgatat ggacgtgcaa
    13921 aaagccgaag agattgttaa actgggtgaa tatttgctgc atctcctcgc aaagcacttc
    13981 cagtttcaaa tagtttccct caagcaataa cgctttggca tgaacgatgt aattaaaacc
    14041 caatccctgg tacaatatcc ggctctgctc catgtccccc gttttaagcc atccggggat
    14101 caaccccggc tcgcccagaa cggctccgac atagcctgag agcaaatcaa cggcgccgta
    14161 taaaacgggg ctgttctcct catccacccc ggcatacaga tcattgatta actctacgac
    14221 ctcggcaaat ctgccttggg ccgcatacaa cctggccagt gtcagattgg cgcagatgat
    14281 aatagaaacc tgcgccatgg tctgggcttt ataaattgtt ttctgcgcgt acgtttcagc
    14341 ctctgcgaag tccccggttt ccaggtaata ttcggcattg accaagtatt caaaccccgt
    14401 tccgcatcca tgagatacct cactgtaata atgaaatact tgatcggcat agtccgcaat
    14461 ccaacgcagc tcaccccgat cccggtaata aagataaagg agatgggggg agccgaaagt
    14521 aaatatcata tctttattag ctattgaaga accgccgtca agcagttggt acgcctttaa
    14581 ctggcattca tgcatctttc ggagatcgtt aaaataaaca aaagtcctgg aaagctcaat
    14641 ctcaccgaag atacgatttt tcaaatcagg aggcgtagct aaatcatccc ggtaatattt
    14701 ttcaatttgg gccaggagtt ccgtcccgtc ctccatatca atgttgctaa gataaaaatc
    14761 ggcgtagagc agatagccga ggggatggcg gtatttcacc gcggggggaa tttgctcaaa
    14821 caactttacg attgactgag ggtaccggtc gactattttg gtgatgccgg gtttttcaaa
    14881 ctccgttaaa atcaggtcgt actccttagc tttcaaaaag gccatcaaac ccgataggac
    14941 atctccattt tgaatatgcc actcaccggc gcgcctgaac agcttttgga gttccgtgcc
    15001 gctaaaccgt tctttcagca gcttttgcaa ataaccgctg aaaatatggt gtattttgta
    15061 ggtttcagtc ctctggtcgt aacggatcaa ggaattgtcc aaacataacc tttggatggt
    15121 tccggcggcg gcagggttgc cggtaacata gacggcctgc tcgggtgtaa aataattaag
    15181 gatacaaagg gcgatcaata actttgcttc ttcaggagta taccgggaca taatcgcttt
    15241 ttcgatcaaa acttcgatat tctttcccat ctccaacttc ccggtctcgc aatacctttg
    15301 aatcatcaaa taaacagctg taatccaacc ctcggagata ccgtgcacct gcccggaagt
    15361 atcatcggaa atatcttttc cgaataactt gaaatactct ttaatttctt gtttagacag
    15421 ttcgaacaaa tcgctctcaa acagacaaca atatcccttc agctgtaact cctcggtgtt
    15481 caactccggc ttggtcctag aaataagcac aatatgcaac ccgctaatat ccgatcggac
    15541 aattctctca agcagccggt ccaattcggg ggagcgggct aaatgatagt catcaattac
    15601 caatactgtg ttggtcgaaa atgcatattc gacaatagcc ccgactactc tatcccgttg
    15661 cggcgcgtca atgggaaaac ccaaagcatt aagcctatct ccgagatccg gtttgacctt
    15721 ggccaactgg cgagttagac aatcccaaat gtattggggg gaagcttcgt cgctgtccac
    15781 ggaaagccag acataatcag cctccgcttc ataaagaaaa gtctttaccg acgtggtttt
    15841 gccgtaaccc atcgccgcca ctacaactgt cagccgataa tcaaaaatct ttttaagctc
    15901 cgcactaatt agcttccgct ttaaaatgcg ttgtttcatt cggatgattt ctccacaatt
    15961 tgtgacgcct tttgctgttc cggcagttgc ccctgggcct gtttctgaat ggtgatgtaa
    16021 ctttttaatg tcttcattct atcataaaat taatgtgtat ttttacattt accggggtaa
    16081 acgactcttt ttatttaaac aatttataag gacagttgac tttgtcctta ggggaaggtg
    16141 taaagtagag ttgaacaaag catttatagt aagaacgaag gtggtttctt gctgtcgatc
    16201 ggggaattct cgaaagtttg cgaagtatcc acaaaaacgc tgcgatatta tgaagaaatc
    16261 ggattaatca ttcctgatga gattaattcc gaaaacgatt acaggtatta ttccgtcagg
    16321 caactccaaa agatgctctt tatcaaccat ctgaaatcat atcatttttc tctggaagaa
    16381 atcaaagcca ttctggaatt ggaagaggac attcccagga ggagcttcgc tcagccctca
    16441 atggcaagag aaaagaaata caggaaaagc tgaatgcttt tgaatatacc ctaaaacaaa
    16501 tgagtcatga tattttgaat ttagaaaagg gcatacccgt catgacttat taccgcattc
    16561 aaagttgagc ttttcatcag ttccgccgga atcaatcgta aaataattca aaattggttt
    16621 caaacatata caattgacac tctttgcctg atgctatata ctatcaatat attcaaacta
    16681 gggagccggt ttttatgata ttcccggtca ttatgtatgt tttagcaata ctgttactaa
    16741 tcatatcatt tacaaaagac gctccgaaaa cgattttagc cttgaaaaag gctttaaata
    16801 tttttgtggg cctattaccg caatttatcg cgattttgct cctggttgga atgattctgg
    16861 cctttttaaa tcccaaaact atcgaggcga tcatcggttc acaatcgggc ttgccagggc
    16921 tgatcctgtc ggcgttaacc ggttcgttta ccatcatgcc ggttttaatc gcctttccga
    16981 ttgcggtcga gttattaaaa aacggagcgg gcctgatgca gatggcggtg ttcatctcaa
    17041 ccctcactac agttggattt atgactatac ccttagaagc caaatatctc ggaatgaagg
    17101 tagccctgtt ccgaaattgc gtattcttca tattctcctt catcagcgct tggatcatcg
    17161 gaatgatatt gtcatgaaaa aaaatctttt gcacgacccc tttttaataa ctgttttagg
    17221 cattaccctc ctgttactta tttttaatcc gcgaatcggt gaaaaatctt tagcgctttc
    17281 ttggaacaac ctgcctagta tcattttcat gttaacaccc atcttcatct tcatgggttt
    17341 actggatgta tgggtgccca aagagaccat gattcgaatc atgggtgaac attcgaaagg
    17401 ccgcggtgct ctgatcgctt tactattagg ggctattacc gcagttccct tatatggatt
    17461 attgccaatg accgggatgt tactgaaaaa aggctgcaaa ctttcaaata tcttaatctt
    17521 tttatgttcc tcctcgagtc tccgaattcc cctgttctta ttcgaagtat cctcgctagg
    17581 ctggcaattt actctaattc aatttctaac cagcctaatc gcagttttgg tcatcgcgtt
    17641 actcgttgaa aaaatcctta ccgaaaagga acgaaccgac atttacaacc gggcccaata
    17701 agatggctac ccctgtaata aaaataaagc taaaagatta ttatagccca ccattactcc
    17761 agatagagct cgatcctttc cacaagcccg gctctaacct gacgggccgg ttcaccggag
    17821 atccgcggcc ctgtaatctc taacttttgg ccgtccctga aataaagcag gcaattgcgg
    17881 actttggccg cgtccgggcc aaaagtcggc cgcagggagg gattctggta gactaccagg
    17941 gtatggccta aaaatatagc cgcgtaatta tccgcagaca attgagcaga aatggcgccc
    18001 tccgtaccgt ttatctccac ctctgctgca gagtggctaa aaaaccggct gtctaagata
    18061 ggttcaaaaa tcaattccag atccccggcg tcattcagcc ggaaaggccg cggaccaaag
    18121 ctcatccaaa gccagagatt tagcatttcg gctgtggagc cgctcaaccg ggccacaaaa
    18181 ccgttgcagt gcatctccgc acggggataa gcgctgctga cgatgaacga acaattctcc
    18241 aatggattcc ggccgtactt tgccggatcg gcgaaacaga ccaggacttc gggaagtgcg
    18301 gcgaaaaact cggaagtttg accgctccgc agaagctcca gtagatactt atactccata
    18361 tgcaagaaga tggattcgtt ctccagccac cccctgggaa aaacccggat ccgtcccagt
    18421 tcttgcggag ccccggagag atcggcgcat accttataca taccaagttc ccggtcaaag
    18481 agagcgcttt cccggaccgc ccggtacaaa tcttgggaac ggtctttgtc ctccatacat
    18541 cgcaatgcat ggacctgccc ttcgagaaaa tggggcaacc ggtattgctc aaatgcttcc
    18601 ggccaaaccg gtatcggtcc ttccggcttt acggtgctcc ccaaggcttg gttgcactcc
    18661 cgccatttac tgactctgtg gtaaaaatag gtatggtaaa ctccgttctc agtgtcaaag
    18721 gaacgagatt cggcatgttt taccaa
//
LOCUS       DASVMB010000003         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_221058,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000003 DASVMB010000000
VERSION     DASVMB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221058"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF531_00125"
     CDS             complement(<1..142)
                     /locus_tag="VF531_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7712398.1"
                     /translation="MRSAYEFAKTAHEGQKRDSGEDFIQHPLEVASLTYDLGMDTTSI
                     MAA"
     gene            complement(209..1936)
                     /gene="recJ"
                     /locus_tag="VF531_00130"
     CDS             complement(209..1936)
                     /gene="recJ"
                     /locus_tag="VF531_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7712399.1"
                     /translation="MGHKVWKVANPLSDQALQLATVLGVSPLLGQLLLNRGITEPETA
                     AAFLKPELSQLHDPFLLPGMERAISRIQQAIRVQEPVLVYGDYDVDGITSVALLIRVM
                     ARLIPGKLLYYIPKRLEEGYGLHTESLEKALDRGVSLIITVDCGISALEEATYLKSRG
                     VDLIITDHHEPPAILPEAYAIINPKLPGGAYPFTQLAGVGVAFKLLQGLVRPFPELEP
                     RLLDNLDLVAFGTVADIVPLVGENRVLVKYGLERLEKTGNAGIQALIEVAAVKNRPVG
                     CSQIGYVLAPRINAAGRMGNPSVAVKLFLTSDPAKTQEYARELENQNQLRQEIENRVL
                     TEAQEQLRNSPGLLDGRALVLAGDNWHLGVIGIVASKLVEIYNKPVILIGLDGDEGRG
                     SGRSIAGFNLFQAVEKCGHFLNQFGGHEFAVGLGIRRDRVSEFREAFMEFAKERLTAE
                     DLTPVLKVESMMPLDGLTLDLVKELNLLAPCGPSNPVPVLGCRSLHLISFKNVGENGK
                     HLKLTVTDDRVVKEAIGFNLGFVNQELAATPEVDVAFSLEENSWNGVVQVQLNLKDVV
                     CNREAIGSE"
     gene            2330..3406
                     /locus_tag="VF531_00135"
     CDS             2330..3406
                     /locus_tag="VF531_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7712400.1"
                     /translation="MTTLKDVAKKAQVSIATVSRVVNGKFPESGISDETRQRVIEAIT
                     ELNYRPDHSARNLRLGNGIKSILFALWAEGNDRDWFIPHPFFSHMLHGIQNEVLSRGY
                     YLSYLALTNQNLGALDALLDGAVAGLITQGPVPETVLDMVAAKRVPLVAIEPYFPLDR
                     DVYPTIYVDNDLAVHQAMEHLVRLGHQRIGFVSIKNESFQLQERRHAFRRWSDYYGLK
                     GFPDQEFISKMEYESADFKGDLDAGAEAISIWSELPDSLRPTAVITANDLIAIGTLRG
                     LRSPENGGWKELSVVGIDDIDWAQYNDPPLTTVRIPKEEIGETAMNLMDRVLQGETIG
                     NYRCVIKTELVVRASTRAIDGVKG"
     gene            3521..>4129
                     /locus_tag="VF531_00140"
     CDS             3521..>4129
                     /locus_tag="VF531_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEX7712401.1"
                     /translation="MILRSEYPRPDLVRHDWIGMNGEWEFAFDDQDQGLVKDWAHDPK
                     SFTKKIIVPFPYQSPLSGIGDPAIHPVIWYHRFFTIPSDWAGKQVIIHFGACDYETFV
                     WINGCLAGSHRGGYASFHMEISALLQPGLNEVTIRVVDRDSISQPRGKQNAKDKPWGC
                     WYTRVSGIWQSVWMEPVSPIHLEHLKLTPDIDRKQLNIRYCLN"
BASE COUNT         1026 a          997 c         1047 g         1059 t
ORIGIN      
        1 aggcagccat aatggaggtg gtatccatgc ccaggtcata agtgagggac gccacctcga
       61 gggggtgttg gataaagtct tctcccgaat cccgtttctg cccctcgtgg gcggttttgg
      121 caaattcata ggcggacctg atcttatgag atctgcgtcc ggtttggtat cgccgatctt
      181 tttcaggagt tcttcgatgg ttaccggttc attcactgcc gatagcctcc cggttacaca
      241 caacatcttt cagatttaac tgtacttgaa ctacgccgtt ccaagagttt tcctcgaggg
      301 agaaggccac gtccacctct ggggtggctg ccaattcctg gttgacgaat cccaagttga
      361 acccaatggc ctccttgacc acacggtcat cggttacggt caatttaaga tgtttcccgt
      421 tttcaccgac atttttaaag gatatgagat ggagcgagcg gcatcccagc acgggtacgg
      481 gattactggg accgcacgga gccagaagat tcaactcctt gaccaaatcc agggtaaggc
      541 catccaaagg catcatactt tccaccttca aaacaggagt taaatcctcc gcggtcagcc
      601 gttccttggc aaactccata aacgcttcgc gaaattcaga aacccggtcc cgacgaattc
      661 ccagtccgac ggcgaattca tgaccaccga attgattcaa aaaatgaccg catttttcca
      721 cggcttgaaa aagattaaat cccgcgatgc tccggcccga accgcggcct tcatccccgt
      781 caagaccgat gaggattacg ggtttattat agatctcaac cagcttggag gcaacgatgc
      841 caatgacacc aagatgccag ttatcgccgg caagcaccag cgcgcggcca tccaacaaac
      901 ccggactgtt ccgtaattgt tcctgggctt ccgtgagaac ccggttttcg atctcctggc
      961 gcagttggtt ttggttctca agttccctgg cgtactcttg cgtcttggct ggatcagagg
     1021 ttaaaaagag tttcacagcc acgctggggt tacccatccg gccggcggca ttgatccggg
     1081 gtgccagtac ataaccgatt tggctgcaac ccacaggacg gttctttacg gcggcaactt
     1141 caattaatgc ctgaattccg gcattccccg tcttttccag tcgttccagg ccgtatttga
     1201 ccaaaactcg gttttcaccc acgagcggta caatatccgc cactgttccg aaagccacca
     1261 gatccagatt gtcaaggagc cggggttcca gctcggggaa gggtctgacc aatccctgca
     1321 ggagtttgaa ggccactccc accccggcca attgggtgaa aggatatgct ccgccgggga
     1381 gtttcgggtt tatgatggca taagcctccg gcaagatcgc cggaggctcg tgatgatcgg
     1441 tgatgatgag gtcgactcct cttgatttga gataggttgc ttcttccaaa gcgctgattc
     1501 cgcaatccac ggtgataatc aggctaaccc cgcggtcaag cgctttttca agggattccg
     1561 tatgcaggcc gtaaccttct tcgagccgct tagggatata gtagagtagt tttcccggaa
     1621 tcagcctggc cattacccgg ataagcaatg ccacggaggt aatcccgtcc acatcataat
     1681 cgccataaac gagcaccggt tcttgaaccc gtatcgcttg ctggattcgg gagatggcac
     1741 gctccatacc ggggagaagg aacggatcat gtaattggct gagttcgggt tttaagaaag
     1801 cggcagccgt ttcaggctcg gtgatgccgc ggttcaataa cagctgaccg agcaatggtg
     1861 agacaccgag aaccgttgcc agttgtaacg cttgatccga taagggattt gcaactttcc
     1921 atactttatg tcccatctta acctcaggtt gacttgaata ttcatccgaa tggatctctt
     1981 ttatttcttt atttcaagca attatcagcg gatcatcttt cgtcaataga tttaacgcta
     2041 actattcttt atccaatcgg ccatttcctg caatttcagg tttaaactta agaggtaatt
     2101 caatgaatca atggaatgaa cggaatgatg cggacagaca cataagctga gcggatcaat
     2161 aaagaaacaa tattaaacgt tttcaagcag ccggatttca gttagccagg gatgttatgg
     2221 tttctgagca aataaattat ttttttgttc gcagaaggag gaattcggtg agaggtggag
     2281 aaattgataa tataagttaa acgtttacaa cgttcttggg agaatcaagt tgacaacctt
     2341 aaaagatgtg gccaaaaagg cccaggtatc cattgccaca gtttcccggg ttgtaaatgg
     2401 caagttcccc gaatccggca tctcggatga gacacggcag cgggttatag aggctattac
     2461 agagttgaat tacaggccgg atcactcggc ccgtaatcta cgcctgggca acggtatcaa
     2521 gtcgatcttg ttcgccttat gggctgaagg gaacgacagg gactggttca ttccccatcc
     2581 attcttcagt catatgttac acggtattca gaacgaggta ttgagcaggg gttactactt
     2641 gtcctatctg gctcttacca atcagaacct cggcgctctc gatgccttac ttgacggagc
     2701 ggtggcgggt ctcatcaccc aaggcccggt tccggaaact gtacttgaca tggtagccgc
     2761 gaagcgggtt ccgttggttg caatcgaacc gtattttcct ttggataggg atgtttatcc
     2821 caccatttat gtggataatg atctggcggt gcatcaagcc atggaacacc tggtccggtt
     2881 gggacatcaa cgaattggct ttgtttcaat aaaaaatgaa tcctttcaat tgcaggagcg
     2941 acggcatgcc ttcaggcgct ggtcggatta ttacggtctc aagggatttc cggatcagga
     3001 gttcatctcg aaaatggagt atgaatcggc cgattttaaa ggagatttag atgccggggc
     3061 agaagcaatt tcgatctggt ctgagcttcc ggattcgctt cgccccacgg ccgtaatcac
     3121 tgcaaatgac ttaattgcga ttggcacact ccggggtctc cgttccccag agaatggggg
     3181 atggaaggaa ttaagcgttg tgggaatcga tgatatcgat tgggcccaat ataacgatcc
     3241 gcctttgact acggttagga ttccgaaaga agagatcggt gagaccgcga tgaatttaat
     3301 ggatcgggtt ctgcagggtg agaccattgg aaattaccgg tgtgtaatca agactgaatt
     3361 ggtggtgaga gcttcaaccc gggctatcga tggggttaaa gggtaacctc tcgcttccaa
     3421 cccgggtaaa ttgaatcgta ataataatgg gtgaagtctg atctttttgt taaacgttta
     3481 caatggtttc tagtttcatc aacagagagg aagaagcaac atgattttac gcagcgagta
     3541 tcctcgcccc gatttagtgc gtcatgattg gatcggaatg aatggcgaat gggaatttgc
     3601 ctttgatgat caggaccaag gcttggtcaa agattgggca catgacccta aatcgtttac
     3661 gaaaaaaatt attgtaccat tcccttatca aagtcccctt agcggtatcg gggatcccgc
     3721 gatccacccc gtcatctggt atcatcgttt tttcaccatt cccagcgatt gggcaggcaa
     3781 acaagtgata atccattttg gagcctgcga ttatgagacg ttcgtatgga tcaacggctg
     3841 tctggccggt tctcaccgcg gcggttatgc ctcgtttcac atggagatct ctgctttatt
     3901 acaaccggga ttgaacgagg tcacgattcg tgtggtggac cgcgacagta tcagtcaacc
     3961 caggggaaag caaaacgcca aggacaaacc ctggggctgc tggtatactc gggtgagcgg
     4021 aatttggcag tcggtatgga tggaaccggt gtctccgatc catctcgaac atctcaaact
     4081 aactcccgat attgaccgga agcaactcaa tatccgttat tgtctcaac
//
LOCUS       DASVMB010000004         8897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_437, whole
            genome shotgun sequence.
ACCESSION   DASVMB010000004 DASVMB010000000
VERSION     DASVMB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VF531_00145"
     CDS             complement(<1..1257)
                     /locus_tag="VF531_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX7712402.1"
                     /translation="MVLERYIPTGNEMISLPDIRERDGAIMGVTFLHMGYKGMIEIRG
                     DSNPDGSDENPLLKPFMMMDGREEPIVQLTWERLNYWIPCFKAKCGNLEISGTILAPV
                     GERGFIYSLKVINRDVAAQEFKLGLRGCWSRTIHEINESKEINAAKYVYRSLWNDSLV
                     FDLRDTVNIFSFAPIFEDQIELTYHQTELSSKKVDFTLGKSMLLAPGEESIVNFYWGF
                     GFEEVGSTTAAKEMLRKGYQYELDATKNWLLKRLRLTGERDMDRILNTNLFFTFFFAS
                     GVTLDTEEFALVTSRSPRYYVSAAYWDRDSLLWSFPSILLVDSEYAKEMLSYAFSKQI
                     KNAGVHSRYIDGTVLEPGFELDELCAPLLALYNYVKHTGDYEILSEESIKKGIARILT
                     TLQSKKHPETDLYETFLQPTDDLNVYP"
     gene            complement(1391..2599)
                     /locus_tag="VF531_00150"
     CDS             complement(1391..2599)
                     /locus_tag="VF531_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7712403.1"
                     /translation="MADVDIEQLKRWRLILGNHSQETLDGYAPGKIALNRDEQIMDDA
                     LAAIYDETDPGSSGQAGSPRSAGLGPSSPHLAQWLADIRTFFSQDLVSVIQADAINRK
                     GLTQLFFEPEILKNVQPDIAMVATLLSLRGRIPEKTKDTARELVKAVVAEITRKLGNE
                     IRQAVTGALNKKNHSPIPSAASIDWKYTISRNLKNYNQEYRMLIPERFYFYGRNQQAN
                     RWTVILDMDQSGSMADSIIYGSVVGSIFAGLPALNTRIVAFDTEVVDLTDQCANDPVE
                     MLFGIQLGGGTDINKSVAYCQQFVKDPARTMFILLTDLYEGGNQASLIKRLEDLHQSG
                     VKVICLLALSDQGAPVYDESSARKISQLGIPCFACTPGLLPEFIEGALKGGDLMALAK
                     QVGGKRSNRH"
     gene            complement(2601..4880)
                     /locus_tag="VF531_00155"
     CDS             complement(2601..4880)
                     /locus_tag="VF531_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEX7712404.1"
                     /translation="MNSVAKGPHIFGIRHLSPGGAYHLRAFLDQVRPTIVLIEGPSDA
                     VAEIQYLTGPEVKPPVAILAYTTELPVRTLLYPFAVYSPEYQALVWAREHQVEARFID
                     LPTAVFLSLESVTGHEDAAAPEGGMGRIYRKWAELAGESDHETYWERYFEHNLNPNAY
                     QTAAWEFGRSLRELDNQSPAETAENLIRESFMRRQIQNALAAGHDSDKIVVVTGAYHA
                     SALATAGEAMTDEEIAGLPRLGSKLTLMPYSYYKLSTQSGYGAGNHGPAYFEILWQYM
                     REGRMDLFPVHYLTELVSYLRENGTYRSPAEVIEAVRLANSLAALHDGSAPTLKDLRD
                     AATVCLGHGDLGVIADAAARTEVGTAIGQLPEGVSQTPVQDDFNRELHRLKLERYKTT
                     VAADLDLDLRENRRVKSEEAAFLDLQRSCFLHRLELLGIPFAHSRQVNQQAATWAEAW
                     VLQWTPEAEIGLVESTLRGETIELAASFLLKERLDAARGVKDAALLIREAGECGLMAS
                     MEQARSVLQGLAVDSGDFLEIAAAARELATVIGYGDIRRYDASYFIPLMQQLFLKGAL
                     LLMDAANCNTEAALAVITGINHLNLVALEHFETVDEELWLAKLTELSRRDDRNPTLSG
                     LACAILLERNRIGNEQLAGEVQRRLSPGIDADLGAGWFEGLALRNRYALLSRNILWEQ
                     LAAYVSSLDELQFRRALVYLRRAFGGFSPAEKRSIAEALGNVWGMSGVDTSDLLNRPL
                     SEEEKQKVESLKDFDFEDI"
     gene            complement(4877..5965)
                     /locus_tag="VF531_00160"
     CDS             complement(4877..5965)
                     /locus_tag="VF531_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7712405.1"
                     /translation="MQNQAILRLPAEILYAGEIQALKAAEKDPVPAGWQMSPRSVLAF
                     ITGGKAGGTEITPKYVGNRRLVEMAIATLVTDRALLLIGEPGTAKSWLSENLAAAIHG
                     DSGKTIQGTAGTTEEQIRYTWNYAMLLAQGPSEAALVKSPIFRAMECGGIVRFEEISR
                     CASEVQDALISILSEKRIAIPELATEVAATKGFSLIATANTRDRGVNEMSAALKRRFN
                     IIVLPTPADLQTEMAIVQKRVQELATNLQLQAALPSDEAFLKVVTIFQELRNGMTLDS
                     KEKIKPPSGVISTAEAISLLTNSMALAGSFGNGKITEADLAAGLQGAIVKDEDKDQAV
                     WLEYLENVMKKRGAEWRSLYKACREMNS"
     gene            complement(6003..6839)
                     /locus_tag="VF531_00165"
     CDS             complement(6003..6839)
                     /locus_tag="VF531_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712406.1"
                     /translation="MSIETLYELDREIGRLMIAGSDLAAGDFRLERLLPPLKQTGTQL
                     PVFARIAGLVEQVIQSPPEQSAGNLLELTNLLHAVLYTQGEIGCPGDLEPITVTTLAG
                     CTRTYVPYRRLRPVIEALTTKGGGRLEIISEADRDGLFQDLRLIHPLIQALDDSYPEV
                     ADLVFEILQRNGESILPLLITDYHPEGGRSDARKLELIAAIKGTGAKELYLEAVERGS
                     TVVKVAAIKALKAVPEAEPLLQDLIKDRKKEIREAATETLKQMHPFAQKIKELKKAIT
                     KS"
     gene            complement(6862..8298)
                     /locus_tag="VF531_00170"
     CDS             complement(6862..8298)
                     /locus_tag="VF531_00170"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX7712407.1"
                     /translation="MKLTKDYIDSLAPNQNAIQNAQGLVNKNHFVSLFQTQDETLLFG
                     ECSGSGSSNYRVSVDFIKPESPVFRCTCPSRQFPCKHSLGLMYALSAGQKFNVGEIPP
                     EVAELREKAEKKAARQEQRKNNPEPAAPKKVNQAALVKKIQAQLQGIDLLEKMLHHLV
                     RSGLGTLDPKSLQIFDEQAKQLGDLYLPGAQNLWLELLWEFEQDEPLETKYNRALPRL
                     LKVYALIRKGREYLTKKSADPLNIVDVETPIEELLGHAWQLTELRDGGRVEKDVELVQ
                     LSFNSCVVEARREFVDTGVWLNLRSGMIQETRNYRPFKASTHIREDDSFFQIVQVKEL
                     FIYPGELNPRIRWEEYTSREFTPADLKKVRSLAAESYADTVRTIKNELKNPLAGKNPV
                     QLLRFSKLGAVCGRPVLENKDGERLVLSDIPQGSEPPSTQLLSLLDKSDLGEGAVLVR
                     FEHDLNARALQAQPLSIISDTGIKRLTY"
     gene            8529..8876
                     /locus_tag="VF531_00175"
     CDS             8529..8876
                     /locus_tag="VF531_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009705214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HEX7712408.1"
                     /translation="MAFSEIELKRIEKLAKSFLDKRRPPVHIRKELDIGYCIDGNSIV
                     VFEIRPLWNNPEKTIEEPVAKATYIKKNDVWKIYWQRSDLKWHGYEADPEVKTLDEFF
                     RIIDEDEYGCFWG"
BASE COUNT         2125 a         2390 c         2216 g         2166 t
ORIGIN      
        1 cggatataca ttgagatcgt cggtgggttg caggaatgtt tcataaagat ccgtttcagg
       61 atgtttcttc gattgcagcg tcgttaatat tctcgcaata ccctttttta tgctctcctc
      121 gcttaagatc tcataatcgc cggtatgttt cacgtaatta tacaacgcca gcaacggagc
      181 gcataactca tccagttcga aaccgggctc taaaaccgtc ccgtcgatat atctgctatg
      241 gactccggca ttcttaatct gttttgaaaa tgcgtagctt aacatttcct tggcatattc
      301 actgtccacc agtaggatcg agggaaaact ccataacagg ctgtcccgat cccagtatgc
      361 ggcacttacg taatatcgcg gacttctgga ggtcaccagc gcaaattcct cggtatccag
      421 ggtaactccg gaggcaaaaa agaaggtgaa gaataaattg gtattcaata tcctgtccat
      481 atcccgctcg ccggtcagcc ttaaacgttt aagtagccaa tttttcgtgg catcaagttc
      541 atattgataa cccttccgca gcatttcctt ggccgccgtg gtcgagccta cctcctcaaa
      601 accgaatccc caatagaaat taacaatcga ttcctcgccc ggcgccaata acatactttt
      661 acccaaggta aagtcaactt ttttgcttga aagctcagtc tgatgataag tcagctcgat
      721 ctgatcctca aagataggcg caaaggaaaa gatatttacg gtatccctaa ggtcaaaaac
      781 cagactgtcg ttccaaaggc ttcgataaac atatttcgcg gcgtttattt ccttactttc
      841 attgatttca tgaatggttc tgctccagca cccccgcaaa cccagcttga attcttgcgc
      901 tgcgacatcc ctgttgatta ctttcagact atagatgaaa cccctctcgc ccaccggcgc
      961 aagaatggtg ccgctaatct ccaaattgcc gcacttcgct ttgaaacacg gtatccagta
     1021 attcaatcgc tcccaggtca actgaacgat cggttcctcc ctgccatcca tcatcataaa
     1081 aggtttcaac aaggggtttt catccgaacc gtccggattt gagtcacccc ggatttcaat
     1141 catgcccttg taccccatat gaagaaaagt gacgcccatg atcgcgccat ccctctcccg
     1201 gatatcgggc aaagaaatca tctcatttcc cgtcgggata tacctctcca gaaccatttg
     1261 tcttcatgcc accttttact ttaaagttga aaaaaatatc aggtttttca atgacctgaa
     1321 agcaactgat aaatttttcc taaaaacaaa tgtaccattc tttctttgat gaatcaagtg
     1381 aaataatcat ttagtgacgg ttactgcgtt ttccacctac ttgcttagcc aatgccatta
     1441 aatccccgcc ttttaacgcg ccttcgataa actccggcaa taatccggga gtacaggcaa
     1501 agcagggaat acccagctgg gagattttgc gggcggatga ttcgtcataa accggggcgc
     1561 cttgatccga gagggcgagc aggcagatta ccttaacgcc tgattgatga agatcttcaa
     1621 gtcgtttaat cagcgaggct tgattgcctc cctcatagag atcggtaagc agaatgaaca
     1681 tggtccgggc cggatccttc acaaactgct ggcagtatgc gacggactta ttgatatccg
     1741 taccgccgcc caactgaatg ccgaagagca tctccaccgg gtcattggcg cactggtcgg
     1801 tgagatccac cacttcggtg tcgaaagcca cgatccgggt attcaatgca ggcagaccgg
     1861 caaagatcga tccaaccacc gagccgtaga tgatggagtc ggccatcgat ccactttgat
     1921 ccatatccag gatgactgtc cagcgattgg cctgttgatt gcggccgtaa aaatagaaac
     1981 gctcgggaat cagcatccgg tattcctggt tataattttt gagattgcga ctgatggtat
     2041 atttccagtc gatactggct gcgctgggaa tgggggaatg attcttttta ttgagcgcgc
     2101 cggtgaccgc ctggcgaatt tcattcccca gcttgcgggt aatctccgcc accaccgcct
     2161 tgaccaattc cctggcggta tctttggttt tttccgggat ccggccccgg agtgaaagca
     2221 gggtggctac catagcgata tcgggttgca cgtttttgag gatctccggc tcgaagaaaa
     2281 gctgggtcaa acccttccgg ttaatggcat cggcttgaat taccgaaacc aaatcctgag
     2341 aaaagaaggt gcggatatcc gccagccatt gggcgagatg gggtgaggaa ggcccaagcc
     2401 cggcagaacg cggcgaacct gcttggccgg atgacccggg atccgtttca tcgtatatcg
     2461 cagccaaggc gtcatccatg atttgctcgt cccggttcag cgcgattttc ccgggggcat
     2521 atccatccag tgtctcctgg gaatggtttc ccagaatcaa gcgccagcgt ttgagctgtt
     2581 cgatatcaac atcagccatg tcaaatatcc tcgaaatcga aatctttcaa tgattcgacc
     2641 ttttgttttt cttcttcgga caaggggcgg ttaagcaggt cgctcgtgtc caccccgctc
     2701 attccccaga cgtttcccag cgcttcggcg atgctgcgtt tttcggccgg actaaaaccg
     2761 ccgaaggccc gccgtaaata caccaaagcc ctgcgaaact gcagttcatc caaggaggat
     2821 acataggcgg ccagctgttc ccaaaggatg ttccgggata acagggcgta ccggttccgc
     2881 aaggccagac cctcaaacca gccggcgccc agatcggcat cgattcccgg ggagagacgc
     2941 ctctggactt ccccggccaa ctgttcatta cctatccggt tgcgttccag caaaatggcg
     3001 caggccagac cggaaagggt cggattgcgg tcgtcccgcc gcgaaagctc cgtcaatttg
     3061 gcgagccaaa gctcttcatc aaccgtttcg aaatgctcca gggccaccag attcaaatgg
     3121 ttaataccgg tgatgactgc cagggccgcc tcggtattac agttggcggc atccatgagc
     3181 aataaagccc ctttcaaaaa cagctgctgc atcaggggga taaaatatga tgcatcatag
     3241 cggcggatat ccccgtaacc aatcaccgtt gccagttccc gcgcagccgc ggcaatctct
     3301 aaaaaatccc ctgaatctac cgccagacct tggagaacgg accgggcttg ttccatggag
     3361 gccatcaagc cgcactcgcc ggcctcccga atcagtaacg cggcgtcttt gaccccccgg
     3421 gccgcgtcca accgttcttt caagagaaac gaggcagcaa gctctatggt ctcgccgcgt
     3481 aaggtggatt ccaccaaacc aatctccgct tcaggcgtcc actgcaagac ccaagcctcg
     3541 gcccaagtgg cagcctgctg gttgacctgc cgggagtggg caaaggggat tcccaacagt
     3601 tccaagcgat gcaaaaaaca ggaacgctgt aagtcgagga aagcggcttc ctccgatttg
     3661 acccggcggt tctcccgcaa gtcgaggtcc agatcggcgg cgactgtagt tttataccgc
     3721 tccagtttta accggtgcag ctcccggtta aaatcgtctt ggaccggagt ctggcttact
     3781 ccttccggca attggccgat ggccgtaccc acttcggtcc gggcagccgc atccgcgata
     3841 acccccaagt ctccgtgtcc caaacagacc gtggccgcat cacgcaaatc cttaagtgtc
     3901 ggagcggagc cgtcgtgcaa ggcggccaaa gagttggcca gccggaccgc ctcaataacc
     3961 tcggccggcg accggtaagt cccgttttcc cgcaggtacg aaacgagttc cgttaaataa
     4021 tgaaccggaa acagatccat ccggccttcc ctcatgtatt gccagaggat ttcaaaatag
     4081 gccggtccat gattgcctgc gccgtaacct gactgcgtcg aaagcttata atacgaataa
     4141 ggcatcaatg ttagtttgct gcccagcctc ggtaatccgg cgatctcctc atccgtcatc
     4201 gcctccccgg ccgttgccag ggcggaagcg tgataagccc cggttaccac tacgatttta
     4261 tcggaatcgt ggccggctgc caaggcattt tgaatttggc ggcgcatgaa ggattcacgg
     4321 atcaaatttt cagccgtttc ggccggagat tgattgtcaa gttcccgcag gctgcggcca
     4381 aattcccaag cagctgtctg ataggcattg ggattcagat tgtgttcaaa ataccgttcc
     4441 cagtatgtct catgatcgct ctcaccggca agctcagccc atttccggta aatacgcccc
     4501 atcccgccct ccggagccgc tgcatcttca tggccggtga ccgactccag agagagaaaa
     4561 acggcagtgg gcagatcgat aaaacgggct tcaacctggt gttccctggc ccataccagg
     4621 gcttggtact ccggagaata aacggcgaaa gggtacagga gagtacgcac cggcaattcc
     4681 gtggtatagg ccaggatcgc taccggtggt ttgacctcgg gaccggtcag gtattggatt
     4741 tcggccactg catcggaagg tccttcgatc aggacgatgg tcggccggac ttgatccaaa
     4801 aaagcccgca gatgataagc ccctccggga gaaaggtggc gaattccaaa gatatggggg
     4861 cctttcgcta ccgagttcaa gaattcatct cccggcaggc tttatagagg ctgcgccatt
     4921 ccgcgccacg ctttttcatc acattttcca gatactcgag ccacacagcc tggtccttat
     4981 cctcgtcttt cacgatggct ccctgaagtc cggcggccaa atccgcctcg gtgatcttgc
     5041 cgttgccgaa acttccagcc agggccatgc tgttggtcaa gagagaaatc gcctcagcag
     5101 tggatatcac accgctgggg ggcttgatct tttccttgct gtcaagggtc atcccgttcc
     5161 gcaactcctg gaagatggtg accactttga ggaaagcctc atcggacgga agggccgcct
     5221 gaagttgcag gttggttgcc aattcctgaa cgcgtttttg aacgatggcc atctccgttt
     5281 gcaggtcggc gggggtcggc aggacaataa tattaaaacg ccgttttaag gcggccgaca
     5341 tctcgttcac accccggtcg cgggtattgg cggtggcgat caaagaaaag cctttggtag
     5401 cggcaacctc cgtagccaac tccgggatgg cgatgcgttt ttcggaaagg atcgagatta
     5461 aggcatcctg aacctccgag gcgcaacggg agatttcttc aaaacggacg atcccgccgc
     5521 attccatggc acggaagatc gggcttttca ccagggccgc ctccgaaggc ccctgggcca
     5581 gcagcatggc gtaattccag gtatagcgga tctgttcctc ggtagttccg gcagttccct
     5641 ggatcgtttt tcccgagtcg ccgtgaatgg cggcggccaa attctcggag agccacgatt
     5701 tagcggttcc cggctcaccg atgaggagca gggcccgatc agtaaccagg gtcgcaatgg
     5761 ccatctccac caagcgccgg ttcccgacat acttgggggt gatctcagtc ccgccggctt
     5821 tcccgccggt tataaaggca agtaccgaac ggggggacat ctgccaaccg gcgggaaccg
     5881 gatccttttc ggcagctttt aaggcttgaa tctccccggc ataaagaatt tcggccggaa
     5941 gccgtaaaat ggcttggttt tgcatggatt caacactcct cgtagtattg aaatgaaagg
     6001 ggttatgact tagttatcgc tttcttgagc tcttttatct tttgagcgaa gggatgcatc
     6061 tgtttcaaag tctcggtcgc ggcctcccgg atctcttttt tacggtcttt aatgaggtcc
     6121 tggaggagcg gttccgcttc cggcacagcc tttaaggctt tgatcgcagc tactttgacc
     6181 acagtcgagc cgcgttctac ggcttccaga taaagctcct tggcgccggt tcccttgatg
     6241 gcggcgatga gctccagttt ccgggcatcg cttctgccgc cttcgggatg gtaatccgta
     6301 atcaacaaag gcaggatact ttccccgtta cgctgcagga tctcaaagac caggtcggcc
     6361 acttcggggt aggaatcatc caaagcctga attaacggat ggatcagacg cagatcctga
     6421 aacaatccgt cccgatcggc ctcgctgatt atctccagac ggccgccgcc cttcgtggtc
     6481 agcgcctcaa taaccggcct caaccgccgg tagggaacat aggtgcgggt acacccggcc
     6541 aaagtcgtaa ccgttatcgg ttccagatca cccgggcatc cgatttcacc ctgggtatac
     6601 agcacggcgt ggagtagatt ggttaactcc agcaaattgc cggccgactg ttccggcggg
     6661 gattggatca cttgctctac taaaccggca atccgtgcga ataccggtaa ttgggttccc
     6721 gtttgtttaa gcgggggtaa cagccgttcc aaccgaaaat ccccggctgc caagtcgctt
     6781 ccggcgatca ttaagcgccc gatttcacga tccagttcat acagtgtctc tatgctcatg
     6841 ggttgaactc cagtcctgca atcaatatgt taaacgtttg atacccgtat ctgaaataat
     6901 gctgagcggt tgggcctgta aggcccgggc attcaaatca tgctcaaacc tgactaaaac
     6961 cgccccctcg ccgaggtcgg acttatccaa caggctcaac aattgggtac ttggaggttc
     7021 ggagccttgc ggaatatcgc ttagcaccag ccgttcgccg tctttgttct ccagtaccgg
     7081 ccggccgcag actgcgccca attttgaaaa acgcaacaat tggaccgggt ttttgccggc
     7141 caaagggttt ttcagctcat ttttgatggt tctaaccgta tcggcgtaag attcggccgc
     7201 cagagaccgg acttttttta aatccgccgg ggtaaattcg cggctggtat actcctccca
     7261 acggattctg gggttaagtt ccccggggta gatgaacagt tctttcacct gaacgatttg
     7321 aaagaaggaa tcatcttccc ggatatgagt tgaagcctta aaaggacgat aattgcgggt
     7381 ttcctggatc atcccggatc gcaggttcag ccagactccg gtatccacaa attcacggcg
     7441 ggcttccacg acacagctgt taaaactgag ctgaaccagc tcgacgtctt tttccacccg
     7501 gccgccatcg cgaagttccg taagctgcca ggcatgtccc agaagctcct cgatgggagt
     7561 ttccacatcc acgatattca agggatcagc cgactttttg gttaagtatt cccggccttt
     7621 acgaatcagg gcgtaaacct tgagtaaacg gggcaaagca cggttatatt tagtttccaa
     7681 tggctcatcc tgttcgaact cccagagcag ttccagccag agattctggg ctcccggcaa
     7741 ataaagatcg ccgagttgct tggcctgctc atcaaagatt tggagcgatt tgggatcgag
     7801 agtacctagc ccggaacgga ccagatgatg aagcatcttt tccagcaagt cgatcccctg
     7861 caactgagcc tggatcttct ttaccaatgc ggcttggtta acctttttcg gtgcggccgg
     7921 ttcggggttg ttttttcgtt gttcctgccg ggccgccttt ttttcggcct tttcccgcag
     7981 ttcggcgact tccggcggaa tttccccgac attaaacttt tggccggcgc tgagagcgta
     8041 catcagacct aacgaatgtt tacatgggaa ttgccggctg ggacaggtac accggaatac
     8101 cggactttcc ggcttgatga aatcgacgga aacccggtag ttggatgaac cgcttcccga
     8161 acactcccca aacaaaagcg tctcatcctg cgtttggaat aagctgacga aatgattctt
     8221 attgaccagg ccttgcgcgt tttggatggc gttttgattt ggagccaagg agtcaatata
     8281 atctttagtg agtttcaaat gtcgttcctc tttcgaaagt ttagaacata tattcgctgt
     8341 ctggatggat tttcctgcat gttgatggtt gttgattatg ttattaattt tcaatgaatt
     8401 aaccgttttt tcaaaaccgg acaaagaaag aaggattttg cgttaagctc atcaaataaa
     8461 acaggaaaac ataaacggag aaagaagaag tatttgtaat ctttgtgaac tcggaaggag
     8521 aaaaaactgt ggctttcagc gaaatcgaac ttaaaagaat cgaaaagctt gccaaatctt
     8581 ttcttgataa acggcggccc ccggttcata tacgaaaaga actggatata ggttattgca
     8641 ttgatggaaa cagtatcgtt gtctttgaaa tcagaccttt atggaataat cccgaaaaga
     8701 caattgaaga gcccgttgcc aaagcgactt atatcaaaaa aaacgatgtc tggaaaatct
     8761 actggcaaag atccgatcta aaatggcacg gttacgaggc agaccctgaa gtaaaaacgt
     8821 tggacgaatt ttttagaatt attgatgagg atgaatatgg ttgtttttgg ggttaaccag
     8881 aattccgcta catttat
//
LOCUS       DASVMB010000005         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_516073,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000005 DASVMB010000000
VERSION     DASVMB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516073"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1035
                     /locus_tag="VF531_00180"
     CDS             88..1035
                     /locus_tag="VF531_00180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7712409.1"
                     /translation="MIEIEKPKITIEETDDHDRYGVFVIEPLERGYGTTLGNSLRRIL
                     LSSLPGYAVTSVKIDGVLHEFSTIPGVVEDTTDIVLNLKQLLVKIHGDCPKKVRLSVK
                     NEKKVTAADIQVSSDIELLNPDLYIATVSSGGSLNAEITIDRGRGFLPAEKNRTIENV
                     IGVIAVDARFSPVQKVNYTIQHTRVGQVTDYDKLILEVWTDGSISPVEAVSLAAKIMT
                     EHLMLFVNLSQTAGKTEIMVEKEEESKNKILEMTIEELDLSVRSYNCLKRAGINTVEE
                     LTRKTEEDMMKVRNLGRKSLEEVEQKINSLGLSLRKSEE"
     gene            1076..1414
                     /gene="rplQ"
                     /locus_tag="VF531_00185"
     CDS             1076..1414
                     /gene="rplQ"
                     /locus_tag="VF531_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7712410.1"
                     /translation="MLKRKLGRESAHRTALFRSMVTALFANEKIMTTEIKAKEARVVA
                     EKMITLAKKGDLHSRRIAAEVINQPEVLQKLFDSIAGRYTERKGGYTRIVKVGPRRGD
                     AAPMVVLELV"
     gene            1563..2324
                     /gene="truA"
                     /locus_tag="VF531_00190"
     CDS             1563..2324
                     /gene="truA"
                     /locus_tag="VF531_00190"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7712411.1"
                     /translation="MTEQEYSSERNLKLIVAYEGTRYAGFQRQRNGLLTIQGVLETEL
                     SNLTGETIRIVGAGRTDSGVHARGQVVNFHTQSRLAPSQFLKALNHLLPPDILIREMG
                     EAVSAFHACHSARSKTYSYRIYNSELRPVFGRNLMYHYRYPLQLEPMQAAADLLVGEH
                     DYRSFQAAGSSAQTTVRTVLFCRLVRQDLELRMEINANGFLYHMVRNIIGSLILVGNG
                     RWSPEDFARVLAAKDRTLAGATAPACGLCLEEVIY"
     gene            2371..>3216
                     /locus_tag="VF531_00195"
     CDS             2371..>3216
                     /locus_tag="VF531_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712412.1"
                     /translation="MWFNPKKFIAYGKWIFRLFFVAGILFCNALLPVGAATENPPGEV
                     MNGKPVVLIIADKLDAEGFFNTQLPGIRRYLETSTVGLMNVRSGVGYVSSSGILTLGS
                     GNRSIFPPDLSVNQRKNQRTTSNDPRSYWEWSLGLLHTSAGGIVVPEIGWILSQSAED
                     GNSGYTGLLGTVAHQHGWRTYLVGNGSNQATSNQPSALLLMDRKGLIDGGDIGSEIER
                     FDPKFPYGYRFDRQRVMTDLRHWIAPKRLIAVNFGDFARLDTYREEMLPQQFERVEQS
                     VWRDFD"
BASE COUNT          830 a          710 c          860 g          816 t
ORIGIN      
        1 cccccattat tccatagaac catatgcgaa tggtatgtaa caaggcgttg gcgcgcgcga
       61 gcgcgtttat agaaggaggg ttaagtattg atcgagatcg aaaagcccaa aattaccata
      121 gaagaaactg atgaccacga ccgttacgga gttttcgtca ttgaaccctt agaaagaggc
      181 tatgggacta cactgggcaa ctcgctccgt cggatccttc tctcttccct accgggttat
      241 gctgtaacat ctgttaaaat tgatggggtg cttcacgagt tttcaaccat tccgggggtt
      301 gttgaggata ccaccgatat tgttttgaac ctaaaacagt tattggtaaa gatccatggt
      361 gattgtccga aaaaggttcg tctcagtgtc aagaatgaaa aaaaagttac cgctgcagac
      421 attcaagtca gcagtgatat tgaactctta aatcccgatc tctatattgc cactgtttcg
      481 agcggtggat ctttaaacgc cgagatcacc attgatcggg gccggggttt tctccctgcg
      541 gagaagaata ggaccatcga gaatgtcatt ggcgtcattg cggtggatgc ccgtttttcg
      601 ccggtccaaa aggtgaacta caccatccag catacacggg tcggtcaagt caccgattat
      661 gataaactaa tcttagaggt ttggacggac ggtagtatca gcccggtgga ggcagtttca
      721 cttgcagcca agatcatgac cgagcatttg atgctctttg tcaatctgag ccagaccgcg
      781 ggcaagaccg agatcatggt tgagaaggaa gaggagtcca agaataagat tctggagatg
      841 accatcgaag aactcgatct ctcggttcgt tcctataatt gcttaaaacg ggccggaatc
      901 aacaccgtcg aggaattaac ccggaagacg gaggaggata tgatgaaagt ccgaaatctg
      961 ggccggaaat ctctggagga agtagaacaa aaaatcaaca gtctcgggct ttcattacgt
     1021 aaatccgagg aatgaactta attgacagtc cgttacagag gaggtatttt gggagatgct
     1081 aaaacgtaaa ctggggcgtg aatcggcgca tcgtactgca ttgttccgca gtatggtcac
     1141 ggccttattc gccaacgaaa agattatgac caccgagatc aaagccaagg aagccagagt
     1201 ggtcgcagag aaaatgatca ccctcgccaa gaaaggcgat ctacattccc ggcgcatcgc
     1261 cgccgaggtc attaaccagc ctgaagtatt gcagaagtta tttgattcga ttgccggccg
     1321 atacaccgaa cgtaagggcg gctatacgcg gatcgtaaaa gtaggcccgc ggcgcggaga
     1381 tgctgcgccc atggttgttt tagaattagt gtaggggcgg atgaggggtc ggctttgaaa
     1441 caacgaagcg gaccccgtgc tttttaaccg ccttttttgg tttgatggat tcctctatat
     1501 aactccggtt ttccttatca aaccacgatc cggttgatca atgccgggta aggagatcag
     1561 acttgacgga gcaggagtat tcatcggaac ggaatttaaa attgattgta gcatacgaag
     1621 ggacccgtta cgcaggtttt caacgacagc gcaacgggtt attgactatt cagggagtcc
     1681 ttgaaaccga gctttccaat ctaaccggag aaacaatccg gatagttggc gccggtcgca
     1741 cagattcagg tgttcatgcc cggggacagg ttgtcaactt tcatacccaa tcaaggttag
     1801 caccttctca attcctgaag gcgctgaacc atcttctccc cccggatatc ctcatccggg
     1861 aaatgggtga agcggtatcc gcttttcatg catgtcattc ggcacgatcg aagacctatt
     1921 cctatcggat ttataactcg gagcttcgtc cggtctttgg ccggaatttg atgtatcatt
     1981 atcgctatcc tttacagttg gaaccgatgc aggctgccgc ggatctactc gtgggagagc
     2041 acgattaccg gagctttcag gcagcgggtt caagtgcgca aaccactgtg cggacggtgc
     2101 ttttctgccg tttggtgcgg caagacttgg aattacgtat ggagattaac gccaacggtt
     2161 ttctctatca tatggtccgg aatattattg gatctttgat tttggtgggt aatggcaggt
     2221 ggtcgccgga ggattttgcc cgggtgttgg cggccaagga ccggaccttg gcgggagcta
     2281 ccgctcctgc ctgcgggctt tgcctggaag aagtaattta ctaaagaacg aagaatgctt
     2341 ggaccaagat gatttggagg agactctgtg atgtggttca atcccaagaa atttatcgcg
     2401 tatggaaaat ggatattcag gttgtttttt gtcgctggga tcctattttg taacgcttta
     2461 ttaccggttg gcgcagcgac ggaaaatcct ccgggagagg ttatgaatgg caagccggtg
     2521 gtgttaatta tcgccgataa gcttgatgcg gaaggtttct tcaatactca gcttccgggt
     2581 atccggcgat atctggaaac aagcaccgtc ggattaatga atgtacgctc cggagtgggc
     2641 tatgtatcca gcagcggcat tttgacttta ggatccggta atcgtagtat ctttcctccg
     2701 gacctgtctg tcaatcagag gaaaaatcaa cggaccacga gcaatgatcc ccgttcttat
     2761 tgggaatgga gtttgggcct cctgcatact tctgccggag gaattgtggt acccgagatt
     2821 ggctggattt tgtcgcaatc agctgaggat gggaattcgg gatataccgg acttctggga
     2881 acagtagctc atcaacatgg atggaggacc tatttggtcg gaaacggctc caatcaagcc
     2941 acatccaatc agccatcagc cctattgcta atggaccgga agggattaat cgatggcggt
     3001 gatatcggat cggagatcga acggtttgat ccgaaatttc cctatggtta ccggttcgac
     3061 cggcaacggg taatgaccga tttgaggcat tggatagcac ccaaaaggct tattgcggta
     3121 aatttcggag actttgcccg acttgataca tatcgtgaag aaatgttacc ccaacaattc
     3181 gaacgtgtcg aacaatcggt ttggcgagac tttgac
//
LOCUS       DASVMB010000006         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_74586,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000006 DASVMB010000000
VERSION     DASVMB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74586"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF531_00200"
     CDS             <1..278
                     /locus_tag="VF531_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712413.1"
                     /translation="RAVLLGIPVIIGKLYIASWMAPVYTRLAQKANLQFQGYHGLITS
                     FLDGGNIIRFWMVKLFIGSWWAWCLIPLAAGLLYYTAWVAGKSRQDQ"
     gene            616..2292
                     /locus_tag="VF531_00205"
     CDS             616..2292
                     /locus_tag="VF531_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX7712414.1"
                     /translation="MKLKGCWLVILTVLNLALLGVLAAHPVRAAGAELSVGLGYSGYL
                     VPGCWMPLRIQVQGAPENAAVEINKLSEDQAHLAAERYPYRGESRLESPLFIDDDFSG
                     VRIRLLAGSEVLAVEELERANIFPGHLVLTLNLPVPLQKAIGTVLLPREPVLVVPVSV
                     TDLPDSEFNYSGVSRLVMVDPGPVLNPAQLQAVRSWLAGGGRLILYAPQDGNESLAQQ
                     LTGRMGPLAALKNSGKVIQTGLGTVKILPATAREDPQAYQGGYWRQLLNIGPYGDNAP
                     LTVAGSFMDSADEHRRIEPRPQKIPLFVWSLAVWSVLTLLAVWLASHKPRWFHPLYLI
                     ILSIVLSIAAIPTGRIIGSLWQRGATSHLRTVFLPGSGPLIHASIYFPQTEGSGRGEE
                     RQGWFSPWGVTVSGKTESGRIEPGLKIAFWNHHEPTPQYIVNSGSDCWLDLTGRSSGM
                     VPAFDSAEEALAWLESKGDITRLALWKENRWFLHRGGGATRETGWMAVEKAPQWVRDE
                     TDWLSRLTAVNPGVVWLVGYGSFSGLDLEIQGGQAGSTCWVTPLPGGMQL"
     gene            2289..2888
                     /locus_tag="VF531_00210"
     CDS             2289..2888
                     /locus_tag="VF531_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712415.1"
                     /translation="MIAVLLLELSDWSRKSGWRTMYLAILAMGLIGFLLALRPEWCCE
                     LLTVIMIWCGLTAGEEYRDPLKNREGITASSLDPWQVLVGKILAALVIGLLHLLAVLP
                     AVVLMVTLWGVPWWILAQVALVSGSAMIAIAGLSIFIYNLWGNDADLFFRLGVGAWMA
                     FTVFFQPLQWFNPFILVWRGLQPNVRRLSCKNFTNMIFD"
BASE COUNT          624 a          703 c          845 g          801 t
ORIGIN      
        1 ttcgtgccgt tctgcttgga atcccagtaa tcatcggcaa actgtacata gcctcctgga
       61 tggccccggt ctataccaga ctggcgcaaa aagcaaattt acagttccaa ggttaccatg
      121 gattgatcac cagtttcttg gatggcggga atatcatccg cttttggatg gtcaagttat
      181 tcatcggttc ctggtgggct tggtgcctga ttccgctagc cgccgggctg ttgtattata
      241 cagcgtgggt agccggtaaa tcccggcagg atcagtaagg cattgttttt attttaatat
      301 ctaccgtagt taagccttgg tggaagcttt tccacgaagg cttttttgtg cccaataccg
      361 gtgccggtaa tcgtggacgc tgcaactgaa ggaattctga gaattttaac gaattatatt
      421 tttaaatcat catcaaacaa ttcaattcga gtaattttag gatttcggtc tcataaaata
      481 cctcaagcca aagaatcatc ggtaacttgg tcccaaatca gtattttttg gggacggggg
      541 ctagtgtcgg tcgaagatat attgcgaatc tagagtttgc gccttcgaag acttcaggat
      601 gcggcggggt gaaatatgaa gctcaaaggc tgctggttgg tgattttaac cgtattgaac
      661 ctggcattgc tgggagttct tgcggcacat ccggttcggg ccgccggggc ggagttgagc
      721 gtgggtttgg gttacagcgg ttatttggta cccgggtgtt ggatgccgct gcgaattcaa
      781 gtccagggcg ctcccgaaaa tgctgctgtg gaaatcaaca agttaagcga ggatcaagca
      841 cacctggcgg cggagcgata cccataccgt ggcgagtccc ggttggaatc ccccctgttt
      901 atcgatgatg atttttccgg ggtgcgcatt cgattgctgg ccggttcgga ggttttggcg
      961 gtcgaggagt tagagcgggc caacatcttt ccgggccatc tcgttctgac cctgaatctt
     1021 ccggttccgc tgcaaaaggc catcggaacc gtgctgcttc cccgggaacc cgtattggtg
     1081 gtgccggttt ccgttacgga tctacccgat tcggagttta attatagcgg cgtaagccgc
     1141 ctggtaatgg tcgaccccgg tccggtgcta aatccggctc agcttcaggc ggttcgtagc
     1201 tggttggccg gtgggggccg actaatccta tatgcccccc aagacggcaa cgaaagcctg
     1261 gcgcagcagc tgacagggag aatgggacct cttgccgcgc ttaagaactc gggaaaggtt
     1321 attcaaaccg gtttagggac ggttaagatc ctgcctgcca cggcccggga agacccccaa
     1381 gcttatcaag gcggatactg gagacaattg ttaaatatcg ggccatacgg agataacgct
     1441 cccctcactg tcgcgggttc attcatggac agtgccgatg aacatcgccg aatcgagccc
     1501 cgaccgcaaa agattcccct cttcgtttgg tcgctcgcgg tttggtccgt gctgacccta
     1561 cttgcagttt ggcttgcgag tcataaaccc cgttggtttc atcctctcta tctaatcatc
     1621 cttagtattg ttttaagtat tgcggctatt cccaccggtc gtatcatcgg aagtctctgg
     1681 caacgcgggg caacctctca cctcaggacg gtttttctac ccggatccgg cccattgatc
     1741 catgcctcga tctattttcc tcaaaccgaa ggttccggtc ggggagagga gcggcaggga
     1801 tggttctctc cctggggcgt tactgtatcc gggaaaacag agtcgggacg gatcgagccg
     1861 ggcctgaaaa tagcattttg gaaccatcac gaaccgaccc ctcaatatat tgtcaattcc
     1921 ggcagtgatt gttggttgga cttgacaggc cgttcttcag gtatggttcc cgcatttgat
     1981 tcggcggaag aggctttagc ctggttggaa tccaagggtg atatcacccg gctcgcgcta
     2041 tggaaagaaa accgttggtt ccttcaccgc ggaggcggag ctacccggga gaccgggtgg
     2101 atggcggttg aaaaagcccc gcagtgggtt cgggatgaaa cggattggct ctcgcgactc
     2161 accgcggtaa accccggcgt tgtctggctt gtgggatacg gttcgttttc cggtttggat
     2221 ttggagatcc aaggcggtca ggccggctcg acctgttggg ttactccgct tccgggaggg
     2281 atgcaactat gattgcggtt ttattgttgg agctctccga ttggagccgg aagtcaggct
     2341 ggcggaccat gtacctggca atcctcgcca tgggattgat cggatttctc ctggcgctta
     2401 ggcctgaatg gtgctgtgag ctactgacgg tgatcatgat ctggtgcgga ctgacggccg
     2461 gggaggaata ccgggaccct ttgaaaaacc gggaaggaat taccgccagt agtttagatc
     2521 catggcaagt actcgtcggg aaaatattgg ccgcgcttgt catcggattg ctgcatctgt
     2581 tggcagttct acccgccgtg gtgttaatgg tgaccctatg gggagtgccc tggtggatat
     2641 tggcgcaagt agcgcttgtc tccggtagcg caatgattgc gatagccggg ctaagcatat
     2701 tcatctataa tctctgggga aatgatgcgg atcttttttt ccgtcttggg gtaggagcat
     2761 ggatggcatt taccgttttc tttcagccgt tacagtggtt taatccgttt atccttgttt
     2821 ggcgtgggct tcaacccaat gtcagaaggt tatcgtgtaa aaattttaca aacatgattt
     2881 ttgattaagt ggaaatttat tcgatttcaa gtttttattg cgaataaagc ttcttccagg
     2941 tcgtatcggt actatattcc gggataactc ttt
//
LOCUS       DASVMB010000007         5624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_369249,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000007 DASVMB010000000
VERSION     DASVMB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369249"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1379)
                     /locus_tag="VF531_00215"
     CDS             complement(30..1379)
                     /locus_tag="VF531_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7712416.1"
                     /translation="MERDSVLNQPNAIQLKEKIQQKKACVGVVGLGYVGLPLAVEKGK
                     VGFTVIGFDQNLKKIDQINKGQNYIKDVKDEELKALVAQGKLTATSDFTKLRDADVII
                     ICVPTPLTVTRDPDISYIVNVTNEIAKYIRKGQLITLESTTYPGTTSEVILPKLEAAG
                     LKVGVDFFLAFSPERVDPGNKRYSTKNTNKVVGGITPACLEIAKTFYEQTIIKVIPVS
                     STEVAELTKVFENTYRAVNIALVNEMMLLCDRMGIDVWEVVDAAATKPFGIQTFYPGP
                     GVGGHCIPIDPFYLTWKAREYDFHTRFIELAGEINIEASYHVVEKITEALGRFKKSLN
                     GARVLVLGVAYKKDIEDFRESPALKVIDLLLKEKAEVIYHDPFVPELPSHPPYTWTMR
                     NTALDEAILKKVDCVAILTDHSDIDYESIVKKAQVVVDARNATKGLSSELKQNVLKI"
     gene            complement(1483..3369)
                     /locus_tag="VF531_00220"
     CDS             complement(1483..3369)
                     /locus_tag="VF531_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX7712417.1"
                     /translation="MKLERNHLLQVLIDILLINLCWFGALVLRFDGRIPGNYLDHWLI
                     IVITMTVVRIGFFIFFRLYRSLWSYSSVPEFFLVLKAVTVSTIVIWAFALTAQELNWL
                     PFPTPKAIGIIDWLLCILSLGGLRFAIRLRREWVISRRNTNNSPQKKLLIVGAGEAGS
                     LIIREISKQLSSNYFPIGFIDDDPCKQGHHMHGIPVLGTREDIPRIIKQYEVEEVIIA
                     LPSASKKDIREILEICHQEVAHVLIVPAVAEIINGTVTMKQLRDVAIEDLLGREPVSV
                     DLTSIAGYLTGERVLITGAGGSIGSELCRQLAPFKPTQLLLLGRGENSIYEIDQELTV
                     DFPELNKVPIIADIRDREKLTQVFRDYRPTVIFHAAAHKHVPLMELEPDEAVKNNVFG
                     TKNMAELADQFKCKRFVLISTDKAVNPSSVMGATKRVAELIIQDLNTRSQTKFMAVRF
                     GNVLGSRGSVIPLFRRQIARGGPITVTHPEMTRFFMTIPEAAQLVIQAGALGHGGEVF
                     ILDMGDPVKIVDLARDLIRLSGFVPDKDIKIEFKGIRPGEKMYEELLTRDEGVRATKH
                     ERIFTTQVKQVDRNVLAERLEILHDHLGDNLLVVRTQLKRLVAEYNQWTGGEKQDVVG
                     SHGS"
     gene            complement(3410..4507)
                     /locus_tag="VF531_00225"
     CDS             complement(3410..4507)
                     /locus_tag="VF531_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX7712418.1"
                     /translation="MIMAAGVGSRLEPLTCNIPKPMVPVVNRPAMEHIIHLLVKNNIT
                     QIAANLWYLPEKIQSYFGDGGKYGVELHYSLEKELMGTAGGVKKLESFLDETFVVISG
                     DALTDIDLWSLIRRHQETGALATLALKEVSDPRQFGVVITDETGRIKAFQEKPRPAEA
                     LSKLANTGIYVFKPEIFKYIPSDTVFDFGKQLFPKLVESGVEIYGHNVKGYWCDIGTL
                     NQYRIANYDVLKGLVKIDIPGSWHPNAVYVGERTIIAPTARIGSKVIVGRNCHIGYGV
                     EIFGETVIGDNCIIEEDTSIFGSIVWNNTQIGRAARLVECVVGSECYLKDGSVIGAGV
                     ILSDDCVVEPGSVIEANSKIWPGNVVGDIIN"
     gene            complement(4925..5458)
                     /locus_tag="VF531_00230"
     CDS             complement(4925..5458)
                     /locus_tag="VF531_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7712419.1"
                     /translation="MPQVALVIAERTFRDEEYQVPKEILSRAGVTVLTACAKLDEAIG
                     KLGLKVHPDTLLRDLAIKDPDALIFIGGGGGSSQYFDDPTAHELARSYVNRGKILGAI
                     CIAPVILANAGVLKGRKATVFPDGKECLAAGGAIYTGNLVEVDRTAPTGACIITGCGP
                     EAAERFGEELVKLVTGD"
BASE COUNT         1595 a         1445 c         1087 g         1497 t
ORIGIN      
        1 gtcaaacact aacgagatta tatgttaact catatcttta aaacattttg ttttaactcg
       61 gaagataatc ccttggttgc atttcgggca tcaactacta cttgggcttt tttgacgatc
      121 gactcataat caatatcgga atgatccgtt aagattgcca cacaatctac cttttttaaa
      181 attgcttcat caagagctgt attccgcatc gtccaagtat agggcggatg ggacggtaac
      241 tccggaacaa atggatcatg atagattact tccgcttttt ccttgagtaa aagatcaata
      301 acctttaagg ccggggattc acggaagtcc tcaatatcct tcttatacgc aacgcccaat
      361 actaacaccc tcgcgccgtt caaagatttt ttaaaacgtc ccagtgcctc agtgatcttt
      421 tccaccacgt gataagaagc ctcaatattg atttcgcctg ccaattctat aaagcgggta
      481 tgaaaatcat attcccgggc tttccaggtt aaataaaacg gatcgattgg tatgcaatgc
      541 ccgccgactc cggggccggg gtaaaaagtt tgaattccaa acggtttggt cgcagcagca
      601 tccactactt cccaaacatc gattcccatt cggtcgcaga gtaacatcat ctcattaacc
      661 agagcgatat tgaccgccct gtaagtgttc tcaaatactt tagttagttc ggccacttca
      721 gttgaagaaa ccggtatcac tttaataatt gtctgttcat aaaaagtttt agctatttcc
      781 agacaggcag gagtgatacc gccgaccact ttgttagtat ttttagtgga ataacgctta
      841 ttgccggggt cgactctttc cggtgaaaac gccaggaaga aatcaactcc aactttcaac
      901 ccagcggcct ccagtttagg caagatcact tccgaggtag taccgggata agtcgtgctt
      961 tccagggtaa ttagttgtcc ttttcggatg tacttagcaa tttcatttgt tacattcacg
     1021 atataagaga tatccggatc ccgggtcaca gtcaatggag tcgggacaca aatgatgata
     1081 acatcagcat cccgcaattt ggtgaaatca gaggttgcag tcagttttcc ctgagcaacc
     1141 aaagctttta attcctcatc cttgacgtcc ttgatataat tttggccctt attgatctga
     1201 tcaatttttt ttagattttg atcaaaccct attaccgtaa aaccaacctt tcctttttca
     1261 acagccaaag gcaaacccac atatcccaaa ccgacgaccc caacgcatgc ttttttttgt
     1321 tgaatcttct ctttcaactg aattgcgtta ggttgattta atacagaatc ccgttccacg
     1381 attaatctcc tccgtcataa tgaaataata tgcgattacc atttcatgta aaaaaatacc
     1441 atgaaaatat gtcatcacaa ccaagaataa aaagacaact acttaagaac cgtggctacc
     1501 aacgacatct tgtttttctc ccccggtcca ttgattgtac tccgctacca accttttgag
     1561 ttgcgttcta acaaccagta gattatcacc caaatgatca tgtaaaattt cgaggcgttc
     1621 cgctaataca ttccgatcaa cttgtttcac ttgagttgtg aaaatccgtt cgtgtttagt
     1681 ggcccgcacg ccctcatccc gggttaacaa ctcctcatac attttttcac cgggccggat
     1741 tcctttaaac tcaatcttaa tatccttatc aggaacaaac ccggagagtc gaatcaaatc
     1801 ccgggccaga tcgacaatct tgaccggatc acccatatct aaaatgaaaa cttctcctcc
     1861 atgtcctagt gcgccagctt gaatgaccaa ctgcgctgct tccggaatgg tcataaaaaa
     1921 gcgggtcatc tcgggatggg ttacagtaat tggtccacca cgggcaatct gtctgcgaaa
     1981 taaaggaatg acgctgccgc ggctacctaa gacatttccg aaacgaaccg ccataaactt
     2041 ggtctggctt cgagtattga gatcctgaat gatcagttcc gccacccgtt tggtggcgcc
     2101 catcaccgag gatggattga ccgctttatc ggtagagata agcacaaacc gtttgcactt
     2161 aaattgatcg gccaattcag ccatattctt ggtaccaaac acattgtttt taactgcttc
     2221 atcgggttcc agttccatta gcggaacatg tttatgagcc gcagcatgaa agatcacagt
     2281 aggccggtaa tcccgaaaca cttgagttag tttttccctg tcgcgaatat cggctataat
     2341 tggaacttta tttagttccg gaaaatccac tgttaactct tgatcaatct cgtaaatcga
     2401 attttctcct cgacccagca ataagagctg agtcggcttg aacggggcaa gctgtcggca
     2461 taattcggag ccgatactac ctccggcccc ggttattaaa acccgctccc cggttaaata
     2521 cccggcaatg ctggtcaaat caacgcttac cggttcccgt ccgagtaaat cctcaatcgc
     2581 cacatcccgt aattgtttca tggtaacagt gccattaata atctcggcaa ccgccgggac
     2641 aatcaaaaca tgagcaactt cctgatggca gatctccaaa atttcccgaa tatctttttt
     2701 tgaagcagac ggaagcgcga taatcacttc ttcaacttca tactgcttaa ttatgcgagg
     2761 aatatcttca cgggttccta aaaccggtat cccatgcata tgatgaccct gtttgcaagg
     2821 atcgtcatcg ataaaaccaa tggggaagta atttgacgat aactgtttag aaatctcccg
     2881 gatgattaga gagcctgcct cacccgcgcc aacgatcagt aacttttttt gcggagaatt
     2941 attcgtgttg cggcgggaaa ttacccattc ccggcgtagt cggatcgcaa acctgagtcc
     3001 gcctaatgat aaaatacata acagccaatc aataatcccg atagcctttg gcgtaggaaa
     3061 aggtaaccaa ttcaattctt gcgcagtaag ggcaaaagcc caaatcacaa tggttgaaac
     3121 tgtaaccgct tttaacacca aaaagaactc cggaacgcta ctataactcc ataagctccg
     3181 ataaagccga aaaaaaatga aaaaaccaat tcttaccact gtcattgtaa tcacaattat
     3241 cagccaatga tcaagataat tgccgggaat cctcccgtca aaccgtaaca ctaacgctcc
     3301 aaaccagcac agattaatga gtaatatatc tattaatact tgcagaaggt gatttcgttc
     3361 taatttcatg tgcatacctt ctaactttcc agtttaaaaa accataacac taattgataa
     3421 tatccccaac cacattaccc ggccagatct tggaattagc ctcgatgacg ctgcccggct
     3481 caacaacaca gtcatcgctc aaaatgactc cggcgccgat tacggaacca tctttcaaat
     3541 agcattcact gcccaccacg cattccacca aacgggcggc gcggccaatc tgggtattat
     3601 tccatacaat gctgccaaag atcgaggtat cctcctcgat gatgcagtta tcgccgatga
     3661 cggtttcgcc gaagatctca accccgtaac cgatgtggca gttacggccg acgatcacct
     3721 tggacccaat ccgagcggtc ggagcaatga tcgtccgctc tcccacatat accgcattgg
     3781 gatgccagga tccggggatg tcgatcttca ccaagccctt taaaacatcg taattggcaa
     3841 tacgatactg atttaaggtg ccaatatcgc accaataccc cttaacattg tggccataaa
     3901 tctcgacgcc ggattccacc aattttggaa aaagttgttt gccaaaatca aaaaccgtat
     3961 ctgacggaat atatttgaat atctccggct taaacacata aatacctgta ttggccaact
     4021 tgctcaaggc ctcagccggt cttggctttt cttgaaaggc tttgattcgt ccggtctcat
     4081 ccgtaatcac taccccgaat tgtctgggat cggaaacttc cttcaaagcc aaggttgcca
     4141 aggccccggt ttcctgatgt ctccggatta gactccagag atcaatatcg gtcaaagcat
     4201 cgccggagat aaccacaaaa gtttcatcca agaacgattc caattttttt accccgccgg
     4261 cggtgcccat cagctccttc tccagagaat aatggagctc gactccgtat ttaccaccgt
     4321 ctccgaagta agactggatc ttctccggaa gataccagag attggcggca atttgggtaa
     4381 tattattttt cactaatagg tgaattatat gctccatcgc cggccggttc accaccggaa
     4441 ccatcggttt cgggatatta caagtcaacg gctccagtcg cgagccgact ccagccgcca
     4501 taatcattgc cttcaacgca aaaattcctc cgcttcatcc aagccgattc catcccaaat
     4561 cgggttatat ccggcttcca ctgttaaaaa ccaagtccaa caagtcatat tttatctttt
     4621 tcgcgatttt cacggctaat cctgcaaact tctgtaaaat tttcggaagc aattttttga
     4681 tttgaaggat ctgcatggaa aaggagtggc attatgtgag gatattcaaa gatgaaaggt
     4741 cgtgcgtagg ttgcgggatg ggcagaaatg aaaaagaagt gctaagtttt gagcgtatct
     4801 ctttgcatca tgaaatgaaa agaaacataa cacgccttta catccatgaa actgtaagag
     4861 acatagtacg ctcttacatc atgaaaaagc attcacctaa taacgaataa ctaggaacta
     4921 ataactaatc accagtaact agcttgacca gttcctcccc gaaacgttcc gccgcctcgg
     4981 ggccgcaacc tgtaatgatg caggcgccgg tgggggcagt tcggtccacc tccaccaagt
     5041 tacctgtata aatcgctccc ccggcagcca aacattcctt accatcggga aagaccgtcg
     5101 ccttcctccc ctttaaaacc ccggcattgg ctaagatcac cggagcaatg cagatagcgc
     5161 cgaggatctt accccgattt acgtaactcc gggcaagctc atgagcggtt ggatcatcaa
     5221 aatattgaga gctgccgccg ccgccgccga taaagatcaa tgcatcagga tcctttatgg
     5281 ccaaatccct caggagagtg tccggatgta ccttaaggcc taatttcccg atagcctcat
     5341 ccagtttcgc acaggcggtc aataccgtca ctcccgcccg gcttaaaatt tcctttggaa
     5401 cctgatactc ttcatcccga aaggttcgtt ccgcaattac cagagccact tggggcatag
     5461 ataggtcacc tcgcatcgaa ataatgatta agaattcgat ataaatagtt ccaaacctgc
     5521 tacgtggaat atcttgcttt cagcgagcgc atatatccgc tcgtaaacct aatcctccaa
     5581 taaatttcac tggtgcctac gactcactct cgctctctct agcg
//
LOCUS       DASVMB010000008         5730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_664243,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000008 DASVMB010000000
VERSION     DASVMB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664243"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF531_00235"
     CDS             complement(<1..329)
                     /locus_tag="VF531_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712420.1"
                     /translation="MSLPEGFTDAAKVHEGENFIVLRARRVQDNSPLILKLPRQEYPA
                     PSEIARLNREYELLRQFKSTEAIKAVGFIVNNHPPALVLEDFGGKALEDIMETKAFEL
                     DEFLEIA"
     gene            complement(820..3417)
                     /locus_tag="VF531_00240"
     CDS             complement(820..3417)
                     /locus_tag="VF531_00240"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7712421.1"
                     /translation="MDEQSALLNRTKLNAPTINQKIIKREKVLKKLRQSPDNKLTLIT
                     APAGSGKTTAAVSFLMEAGIPFSWLSLDESDNDPVRFWKYLIAACRGLGNFGENFWDV
                     PVKQELIVSNIQTDILLDKLYTIPGKAFIVLDDYHLIYNEIIRSNLVYFLKYLPSHVR
                     VMILGRKEPDTKFIHERANGQILKLGVRDLSFDHREVAEFFNVKGWDLTSEEIAAICD
                     YTEGWAAGLVMTSLSMEEEGDAHAAVSRFSGKNRHIDLLFQDEVFERWPDEVKDFLVH
                     ISFLDKFCGPLCEAVTGIPDSAEYLKKLAQGNSFIFYLDQENEWFRFHHLFGEFLQQR
                     LAREDQAVRREMYLKAGKWYRENGFVREAIEVFIKAEAFQEAFPLLLDMNFYLSMVQN
                     GDFSAWLTWMGCIPVEYYEGEVRACTGYSWVLSMENRTDEAEFWADKAQSCFDRIKDG
                     IAADEKDFLAANVIMAKANIAILEMDMERAGYYYKQAGGLQLYQPIVIGELNSGEASI
                     LKTAYGFRGRLKKFDELYALLVSELPRLIGNFSAYLMVGLAECHYERNNRETAYQTLT
                     QVMENIIELGNPGVIVPCVITLAKIKRVEGDIEGAIQTITEGREKLAGKSKTFWNYYL
                     DVFTAGLYLDRHNAAAAAEWLNIDRIGVFDNLSCTREYEYLTFARYLNLVGRYDDALL
                     LLNRLNTFAQKEDRLGSRIEILCRIAVGYHLKKDSANAMTALDEALALGMEDGYLRTF
                     IDQWEPMAELLAKYKNWKKRTGTDARSNYAKELFRLVQENILAIKTKLPEVREVPLLT
                     PLAAHLSVREFRILRLLAADRSNKEIAAELCISVRTVKYHNSQIFEKLGVENRIQALN
                     KAWETGILE"
     gene            complement(3682..3918)
                     /locus_tag="VF531_00245"
     CDS             complement(3682..3918)
                     /locus_tag="VF531_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7712422.1"
                     /translation="MIRLFFIGNANALGFYDMSGNVMEWCFDKNDSGRVERGGSWAHR
                     FSWMMIGNVQFYDPAYYFVHISNGYVGFRPVRTN"
     gene            complement(3912..4100)
                     /locus_tag="VF531_00250"
     CDS             complement(3912..4100)
                     /locus_tag="VF531_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HEX7712423.1"
                     /translation="MNKKLHLVQSQKRGNVIAGGCLHSLALTSNGTVYVWGWNEDGQL
                     GNGSSDYYSNTPLKVSGL"
     gene            complement(4317..4559)
                     /locus_tag="VF531_00255"
     CDS             complement(4317..4559)
                     /locus_tag="VF531_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712424.1"
                     /translation="MRKKCLPFNLALILVLAFALAGCSGNNNNNTGGTTIYPTIYTAT
                     TGGLSISKDGGTTFTNYTKETNGLGSNFVAAVYVDP"
     gene            complement(4862..>5730)
                     /locus_tag="VF531_00260"
     CDS             complement(4862..>5730)
                     /locus_tag="VF531_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712425.1"
                     /translation="VMNQPGPAVSVRVAIFLLALIASVFLNGCGGGSGGGNSGDNNNN
                     PTYKSLGVGVTIQSSSDPKTQSMSYQATGFDWKRIDLPLNAPRVQMAPVFRMTDFSSS
                     AGLDPAKNIYLILLAWDKVDGAGHYQVFYQGNKVWDSDTFDTIADGAFDKDNPQAYLD
                     FDDELKTGTLAAGEYQFEIKALDGNTVIAELPKVNASLGVYLQAIPEIGDFNVTKPNL
                     TWNAITNTTEYRISFNGVNTATVGSSTLSYDVGQKLTASATYYRVWIDARYSDANGNP
                     VEIARGRGGLSY"
BASE COUNT         1523 a         1463 c         1169 g         1575 t
ORIGIN      
        1 aaagcgattt cgagaaactc atccaactca aaagcctttg tttccatgat atcttccagc
       61 gctttaccgc cgaaatcttc cagcaccagg gccgggggat ggttatttac gatgaatccg
      121 actgctttga tggcctccgt cgatttgaac tgccgcaata gttcatattc ccggttcaag
      181 cgcgcaattt ccgaaggagc ggggtattct tgccgcggca gtttcaaaat cagcgggcta
      241 ttgtcctgca cccgccttgc acgtaaaaca atgaaattct cgccttcatg tactttggcc
      301 gcgtcggtaa acccctcagg aagtgacatt ttggcaaact ccttgctttt cgctaactca
      361 gcttcaactt tgcgccaaga aaaagatgat ttggcctata aacatgctta aaatatagca
      421 aaaatcagga gaaaagtaca ggtatttgcg agtgttgaat tactttctat ccttttgcga
      481 tattgtacaa taagcaatca ctaagaaacc gccctacata atcttccgaa caataattaa
      541 aattgataaa cgttccgata ataaccgtac cgctacttcc gtcaaaatcc ataacaaaga
      601 gcccgcgctt atagcattcc ataaaaaacg gccgctgcgt atttatcgcg caacagccca
      661 aacttcacac tttcgatgcc cctttcccag cttggtcttt ctcccaagac atgatagttc
      721 tctccactcc gaacagggag acgacgaaaa aaacggggct taaccaacaa aattccgcca
      781 acattccggc taatcctaaa aatcgggtga atcctggtcc tattccagta ttccggtttc
      841 ccaggcttta ttgagggctt ggattcggtt ttcgaccccc aatttctcga atatttgcga
      901 gttatgatat tttacggtcc gtacggatat gcaaagttcc gcggcgatct ccttattgga
      961 acgatccgcc gccagcagcc gcaaaatccg gaattccctg acggagagat gcgcggcaag
     1021 gggagtcaaa aggggaacct cccggacctc cggaagcttg gtcttaatcg ccagaatatt
     1081 ctcctgtacc aaacggaaga gttctttggc gtaattacta cgcgcatccg tccctgtcct
     1141 tttcttccag tttttatatt tggccaacag ctccgccatg ggttcccact ggtcaatgaa
     1201 cgtccggaga taaccatcct ccatcccgag ggccagagcc tcatccaaag ccgtcatcgc
     1261 gttagccgag tcctttttca gatgatagcc gacggcgatc cggcatagga tttcaatcct
     1321 gcttcccagg cggtcttctt tttgggcaaa agtgtttagc ctgttcagga gcagtagggc
     1381 gtcgtcatag cggccaacca ggtttaaata tctggcgaaa gtcaggtatt catactcccg
     1441 ggtacaggaa aggttatcaa ataccccgat ccggtcgatg ttcaaccatt ccgcggccgc
     1501 agccgcatta tgccggtcga ggtaaaggcc ggcggtaaag acatccaaat agtaattcca
     1561 aaaggtttta ctcttgcccg ccaatttttc ccgtccttcg gtgatagttt gaatggcccc
     1621 ctcaatatcg ccctccaccc ttttaatttt ggccaaagta atcacgcaag ggacgatgac
     1681 accgggattt ccgagctcaa tgatgttttc catcacttga gtcaaggtct ggtaggcggt
     1741 ttcccgatta tttcgttcat agtggcattc ggccagcccg accattagat atgccgaaaa
     1801 attcccgatc agccggggga gttcagagac caaaagagca tagagctcgt cgaacttttt
     1861 cagtctgcct cgaaagccgt aagctgtctt taagatgctg gcctccccgg agttcagctc
     1921 tccgatcaca attggctggt acaattgtaa cccgccggct tgtttgtaat aataacccgc
     1981 ccgttccata tccatttcca ggatggcgat attcgccttg gccatgatta cattcgctgc
     2041 caaaaaatct ttttcatccg cggcgatacc gtccttgatc cggtcaaaac aggactgcgc
     2101 cttgtccgcc caaaattcgg cctcatctgt ccggttttcc atcgataaca cccacgaata
     2161 cccggtacag gccctgactt ctccttcgta atattctacc ggaatacaac ccatccacgt
     2221 gagccaagcg gaaaagtcac cgttttgtac catggagaga taaaaattca tatccagcaa
     2281 taacggaaaa gcctcttgaa aggcctcggc ctttatgaat acctcaatgg cctcccgtac
     2341 aaatccgttt tcccggtacc actttccggc tttcagatac atctcccgcc ttacggcttg
     2401 atcttccctc gccagccgct gctgtaagaa ttcgccgaaa agatggtgga agcggaacca
     2461 ttcgttctcc tgatccaggt aaaaaatgaa gctattgccc tgggccagtt tttttaaata
     2521 ttccgcactg tccgggatac cggttacagc ctcgcataac ggtccgcaaa atttatccag
     2581 gaaagaaata tgtaccaaaa agtctttcac ttcatccggc cagcgttcga agacttcatc
     2641 ttgaaaaagt agatcaatat ggcggttttt ccccgaaaac cggctgacag ccgcatgcgc
     2701 atcgccctct tcctccatgg aaagagaggt catcaccagt cccgccgccc agccttcggt
     2761 ataatcgcaa atggccgcga tttcttccga agtcaaatcc caccctttga cattgaaaaa
     2821 ttcggcgacc tcccgatggt caaaggatag atcacggacg ccgagtttca ggatctgccc
     2881 gttagccctt tcgtgtatga attttgtatc cggttccttg cggcctaaga tcatcacccg
     2941 gacatgggag ggtaagtatt taagaaaata gaccaggttg ctccggatga tttcattata
     3001 aattaagtga taatcatcca gcactatgaa tgcttttccg gggatggtgt agagtttatc
     3061 gagcaagatg tctgtctgga tgttggaaac gattaattcc tgttttaccg gtacgtccca
     3121 aaaattttcg ccgaagtttc ctaacccccg gcaggcggcg attagatact tccagaaccg
     3181 gaccggatca ttgtcgcttt cgtcaaggga tagccaggaa aagggaatgc cggcttccat
     3241 caaaaaacta acggcggcag tcgtcttgcc ggagcctgcc ggcgctgtaa tcagtgttaa
     3301 tttattatcc ggggattgtc gcagcttttt gaggactttt tcccgtttga tgattttctg
     3361 attgattgtc ggcgcattca gcttggtccg gttcaatagt gcgctttgtt catccattca
     3421 aatttagcct tcttggtgtc caaaaattta ggttattttt tatgatattt cggtacaaaa
     3481 aagacaattt cctgcatcta ctacaattta aaaccagtct cgattaaatc aatccatttt
     3541 attaacagag ttcttttaat tgtaaataat cacaaaagtt aaaaaataac ctaccaccaa
     3601 attctttggc cggtaggttt agttgttttt ttaacttaaa atgctttaat taaaacagat
     3661 atatctaaaa ttttagcttt ttcaattcgt ccttacggga cgaaaaccaa cgtagccgtt
     3721 agaaatgtgg acgaaatagt aggccggatc atagaattgc acgttgccaa tcatcatcca
     3781 gctaaatcta tgggcccatg aaccgccacg ctccaccctg ccgctatcat tcttatcaaa
     3841 acaccactcc atgacgttac cgctcatgtc ataaaagcct aaagcattag catttccgat
     3901 aaaaaatagt cttataatcc gctgactttc aacggggtat tagaataata atcactgctt
     3961 ccgttcccca actggccatc ttcattccat ccccagacat aaaccgtccc gttactggtt
     4021 aaggccaggc tatggagaca tcccccggca ataacatttc cccttttttg tgactgtacc
     4081 aagtgcagct ttttattcat tattatcaat ggattccttg atttttttaa atttaacaac
     4141 cggaagtagt gaattttcat attaaattaa aattaactcc atttaaatta ggatagaagc
     4201 ggtgattaac cgccgacctc cggatagaag ggctgcgcaa ctctcacacc gcctgtaccg
     4261 ttcggtatac ggtggtcctt tagtttatac gggcttgtcg atcagacaat gcctgatcag
     4321 ggatcaacat aaacggccgc tacaaagtta ctccccaatc cattagtctc tttggtataa
     4381 ttggtaaagg ttgtcccgcc gtccttggaa atcgataatc cgccagtagt cgccgtgtaa
     4441 atagttgggt aaatagttgt gccgccagta ttattgttat tgttcccgct acaaccggcc
     4501 aaagcgaacg ccaatactag aatcaacgcc aaattaaaag gtaaacactt ttttctcatt
     4561 ttgaaatttc ctccgattgc agcatttcaa aaactgttcg tcgacatttt cactatctgt
     4621 tatcaatcaa atattgtaaa agttggtcac atatcatatg taatcaacct aatgcgaaaa
     4681 ttaagattca catacaagac tgccgccgcc gactagacgg cgctgcagag caagatcagg
     4741 acaatcaaca gggttgtcca ggatttggct ttaacgaggt tcatcatcat cctcttgaat
     4801 ccgaactaag tcgaattgct atctttttac taaaatatat ctcaactgaa tctcaactga
     4861 attaataact aagcccgcct ctccccctgg caatctccac tggattgccg ttagcgtccg
     4921 agtatctggc gtcaatccaa acccggtaat atgttgcgga agctgttaat ttttgcccta
     4981 catcataaga aagagtactg gatccaaccg tagccgtatt aacaccatta aagctgatcc
     5041 ggtattcagt ggtgttagtt atggcgttcc aggtcaggtt gggtttagta acattaaaat
     5101 caccaatctc cggtattgct tgcagatata ctcccagcga agcattgact ttcggcaatt
     5161 cagcaatgac ggtattaccg tctaaagctt taatctcgaa ctgatactct ccggcggcaa
     5221 gagtccctgt ctttagctcg tcgtcgaaat ccagatatgc ttgaggatta tccttatcaa
     5281 aggctccatc ggcgatggta tcaaaggtat ccgaatccca aaccttattt ccttggtaaa
     5341 atacttgata gtgaccggcc ccgtccactt tatcccaggc taatagtatc aggtaaatat
     5401 ttttggccgg atcaagcccg gcgctactac tgaaatctgt catccggaat accggggcca
     5461 tttgaaccct gggggcatta agcggcagat caatccgttt ccagtcgaaa ccggtagctt
     5521 ggtaactcat gctttgcgtc ttcgggtcgg aactgctctg aatagtcacc ccgacaccca
     5581 gcgatttata ggtgggattg ttattgttat cgccgctgtt tccaccgcca ctacctccac
     5641 cacagccgtt taaaaagaca ctggcaatta aagccagcag aaaaatagcg actcttacac
     5701 tcaccgccgg tcctggctga ttcatgactc
//
LOCUS       DASVMB010000009         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_75668,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000009 DASVMB010000000
VERSION     DASVMB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75668"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..758)
                     /locus_tag="VF531_00265"
     CDS             complement(195..758)
                     /locus_tag="VF531_00265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7712426.1"
                     /translation="MAELVFRGTPELETGRLSLRRLAPGDAADIFEYASDDEVTKYLT
                     WETHQSIENARGFINFTLGRYERDEAGDWGIILKETGKLVGATGFAWYDSKNNRAEIG
                     YVLARPYWGRGIMPEALSRLLEFGFEEMRLNRIECCHFLPNEKSGRVMQKAGMSYEGI
                     ARDRAFAKGKYWDVKQYAILRSDWINR"
     gene            1075..1644
                     /locus_tag="VF531_00270"
     CDS             1075..1644
                     /locus_tag="VF531_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712427.1"
                     /translation="METNRSIKVLSFICLVLMITSPGQITAAQAERTFTPAELAGCNG
                     QNGAPAYAAVNGLVYDLTNVRGWSGGKHFCPGALAGRDLSAIWNLIPRSHRNPNFLKR
                     FTVVGRLVATKPELATPAPSGGISGPQATAVPSPALVPSPTTVQPRPSLSPSPETPGG
                     TSITKVLITGLIFAGLISLLWVAIRMKRR"
     gene            complement(1683..2954)
                     /locus_tag="VF531_00275"
     CDS             complement(1683..2954)
                     /locus_tag="VF531_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEX7712428.1"
                     /translation="MKPRQLLLVVLFIALAFPLVFGGVAVPGVVGGSSSIYSLSRLGG
                     LLGMILFGMLFVLGTKPKFIETFFGHDKMIALHRKLCVTALVLVLFHGIVGIWNAYGG
                     GTGLLLGVVAFFLLIITFLFIVLQKIFHWAYEFWTTIHQLTYILFPPAYLHSLLLGTS
                     LRSSLVWQIIWVLLGIEYLGILGYKIWNARQNRKNRFQVTQVIQETPNIWTLQFQPEG
                     GSPFSYLPGQFLILYLSQAGRMSDPHPFTIASSPTREILSVSIKEAGDFTGNIGRTIT
                     GDYALIDGPYGRFSFLHYPAEEYIFIAGGIGITPFLSMLRYMRDRRIKKKILLLWGNR
                     TERDIAFREELNRMERTMTGLKVVHVLSDQGDWRGESGFINASLIQKYGDIRGQEIFL
                     CGPPLMMKLVQKELRSFNLPKQQIHFERFAW"
BASE COUNT          883 a          898 c          739 g          869 t
ORIGIN      
        1 tagatttaat ttttgtcccg caaaattcat aattgctgaa cttgcttttg gcattatcca
       61 atgaatttta gtgggttaat tttattttaa aacgatatga cagccgagaa aatggaaaaa
      121 ggtaattaaa atatgggata atgtcctgtg cggtatggtt atgttgcggg atttcggttt
      181 tatctataaa gtatttacct gttaatccaa tccgagcgca ggatggcgta ttgcttcaca
      241 tcccagtact ttcccttggc aaatgcccgg tccctggcta taccctcgta actcatcccg
      301 gccttctgca tcacccgtcc tgatttctcg ttgggcaaaa agtgacagca ttcgatgcgg
      361 ttcaacctca tctcttcaaa tccaaactca agtaaccggc taagcgcttc cggcataatc
      421 ccccggcccc aatagggtct ggcgagcaca tatccgatct ccgcccggtt attcttgctg
      481 tcgtaccagg caaaccccgt ggccccaacc agttttccgg tctcttttaa aatgatgccc
      541 caatctccgg cttcgtcccg ttcatacctt cccaaggtaa aatttatgaa tcccctggcg
      601 ttttcgatgg actgatgggt ctcccaggtc aggtatttcg ttacctcatc atccgaggcg
      661 tactcaaaga tatcggcagc atcaccaggt gctagcctcc tcagggataa acgcccggtc
      721 tcaagctccg gggtacccct aaaaaccagt tccgccattt atgtctcccc tttcttccga
      781 ttgattctca atgttcatcg tactcatgat ggaggtactg ttcgatactg ctcatgcaat
      841 cacctcccct cggaagatag aaaaaaaacc gcgtcaatca gcagttagcg attccgcggg
      901 atatcattct acttaaggcg aattcacctg attatattaa tatgcttaca ttcagttttt
      961 taaggacgcc gttttctttg gcagacacga ggctacttgg gcaaaagaat taatctggaa
     1021 tacccgtaag ttaccagggt aagctaaata aagcaattca tggaggatca tttgatggaa
     1081 accaaccgaa gcataaaagt attgtcattc atctgtttgg tcttgatgat taccagtccc
     1141 ggccagatca cggcggctca agccgaacgc acctttaccc ctgccgaact tgccggatgc
     1201 aatggtcaaa acggcgcacc ggcctatgcg gctgtaaacg gcctggttta tgatctgacc
     1261 aatgtccgcg gatggtcagg aggcaagcat ttctgtcccg gagcgctggc cggaagagac
     1321 ttgagcgcca tctggaacct gatcccccgt tcccaccgca accccaattt tttgaagcgt
     1381 tttacggttg tcggaagact ggtggccact aagcctgaac ttgccacccc cgcgccctcc
     1441 ggcggcattt ccgggccaca agcgactgcg gtcccctctc cggctttggt tccatctcca
     1501 accactgttc aaccccggcc ttcgttatct ccttcaccgg aaacacccgg cggaacatcc
     1561 atcaccaagg tcttgattac agggttgatc tttgccggac tcatcagcct cctttgggtg
     1621 gcgatccgta tgaaaaggag atgaatcgcg cacatacgca cgcacatatg cgcgtatact
     1681 ttttaccagg caaaccgttc aaaatggatt tgctgtttgg ggaggttgaa agaacgcagc
     1741 tctttttgga ccagtttcat catgaggggc ggcccgcata ggaagatttc ttgaccgcgg
     1801 atatcgccat acttctgtat cagtgaagca tttataaaac cgctctcacc tcgccaatcg
     1861 ccctgatccg acaacacatg caccactttt aaaccagtca tggttcgttc catccggttc
     1921 agctcttcgc gaaaagcaat atcccgttcc gtccggttac cccataacag taagatcttc
     1981 ttctttattc gccgatccct catgtaccgg agcatgctca gaaaaggagt aatcccgatg
     2041 ccgccggcaa tgaagatata ttcttccgcc ggataatgca gaaagctaaa tctgccgtat
     2101 ggcccgtcaa tcaaagcgta atcccccgtt atggtccggc cgatattacc ggtaaaatct
     2161 ccggcctcct taatcgaaac cgaaaggatt tctctggtcg gactcgaggc gatggtgaag
     2221 ggatgagggt cgctcattct gccggcctgg gacagataca agatcagaaa ctgtccgggt
     2281 agataagaaa aggggctacc cccctccggc tgaaattgaa gggtccaaat attcggggtc
     2341 tcctgaatta cctgggtgac ttgaaaacga ttcttcctgt tctgccgggc gttccagatt
     2401 ttataaccga ggatccccag atattcgata cccaataaca cccagataat ctgccatacc
     2461 aagctactcc gcagactggt gccaagcagc agactgtgaa ggtaggcagg gggaaagagg
     2521 atgtaagtga gctgatgaat ggtggtccag aactcgtagg cccagtgaaa gatcttctgc
     2581 agtacgataa ataagaacgt gatgatcaag agaaagaacg ccaccactcc cagtaacaac
     2641 ccggttcccc cgccataggc gttccaaatc ccaacaattc cgtgaaacag aaccagcacc
     2701 aaggcagtga cgcacaattt ccggtgtagg gcgatcattt tatcgtggcc aaaaaaagtc
     2761 tcgatgaatt tcggtttggt tcctaaaaca aatagcatac caaatagaat catgccaagc
     2821 aaaccgccaa gccgggagag gctataaatg gaggacgatc cacctaccac ccccggtaca
     2881 gccacgcccc cgaacaccaa cgggaatgcc agcgcgataa ataacactac cagaagtagt
     2941 tgcctgggct tcatcaaaac tccccttcac gcgacggctc attgttgttc agcacaaatt
     3001 caagtaaaaa ccacctttca tgtatctttc ccagcccgga tttttttaaa gccatgttct
     3061 tgtaaacctt gtattaccaa gagtggggtt aaaagcttaa aaatctttcc atgttctgtt
     3121 tcgtggattt cgtgtttacg gttcccttcc ttgacgattt tgtgggtaac acaaccacgg
     3181 attacacgaa aggtcacgaa ataaaatacc cttttcgtga atttcgtgtt ttcagttccg
     3241 ttccttgacg attttgtgag caatacaaac cacgtttaca cgaaaggtca cgaaataaaa
     3301 tacccttttc gtgaatttcg tgttttcagt tccgttcctt gacgattttg tgagcaatac
     3361 aaaccacgtt tacacgaaag gtcacgaaa
//
LOCUS       DASVMB010000010         3939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_296243,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000010 DASVMB010000000
VERSION     DASVMB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296243"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VF531_00280"
     CDS             complement(<1..1224)
                     /locus_tag="VF531_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7712429.1"
                     /translation="MMAEIKRASMHDAYTRNRRGKRPLHLLMLGLIWLGMTTVWPVTS
                     QAASGGLHFTRTTLKNGLTVLYKVMKNEPLVSIYAVFPIGMNREKAKGIAHLLEHVVF
                     RGGSGYNFTDIAEVTTRKGGQFNGFTSFDTTSYNFVVPRENLSEGLRIFNGIVWKPIT
                     SGTALELEKKIVIHELDMDYSERYQEYPVYRYFYPEFSYSQASVAAITSQDLEEFHRQ
                     FYQPDQATYVLAGDFDPRQVLPELEKLGNGYGGRIGAEPETTGFDLPDRDLVEERNLY
                     PYQYQILMAYQFNGMTPQERMVLKLLANLYRVDAKIDYEKNEYRLYNTIYRNVGARDY
                     FGIYYLERSHPYSEAVFKEDQAALSKYFREFAAIDLRQSLEDTIRQINLAAASSEQSA
                     SAAVEYEVTRLTDPDT"
     gene            complement(1208..2032)
                     /locus_tag="VF531_00285"
     CDS             complement(1208..2032)
                     /locus_tag="VF531_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712430.1"
                     /translation="MVKFARYLLIVTGIWLGAGIGSAANLPGDNLVSGSYPLIAVTNH
                     TPTHYKNGQAYWDALQEVKLTVGGQEQFRDLPPLGMKSPFTGMIVMGDQPQKFGVIAD
                     INGGEKRLYVDTDGDGSFANAPWFPLLDEWQGLQIYWVESPDPITLQVNYQAHRDGSR
                     PIQIMVSGLLNRPGPLVKDQPYLRIEVRTWFLAKLEETGGTGLAAVVDRNHNGRYDDS
                     GDELYIDRNNDGYFETDEAISRKQGIELRSGNRRLPVNWEAYPERLVIGGNNDGRD"
     gene            complement(2082..2639)
                     /locus_tag="VF531_00290"
     CDS             complement(2082..2639)
                     /locus_tag="VF531_00290"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7712431.1"
                     /translation="MRKITKQGGTVLAPVPVVMVSCAAPEYRPNIITIAWTGLLCSDP
                     VIVSISVRPERYSHAIISQSREFVINLPSKELLRVTDYCGMVSGRDQDKFAVCGLTPV
                     SAKQVQAPLIAEAPVNLECKVSQVIPLGSHDLFIAEMVQAHYAETVLDARGVLDLHAL
                     PGVAYSNGSYYQVGEALGTYGFSRK"
     gene            complement(2636..3004)
                     /locus_tag="VF531_00295"
     CDS             complement(2636..3004)
                     /locus_tag="VF531_00295"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEX7712432.1"
                     /translation="MKPQELAKQLAASLAESEEYRKYRAAKQVLDEHEAAKSMFEDFR
                     KKQLELERKRLSGEKLLEPYETELKKLAEIVGLNPYVREYLMAEYQFSQLMMVIQRTI
                     ADAVGIELPQDTGSEGGDSQ"
     gene            3185..3874
                     /locus_tag="VF531_00300"
     CDS             3185..3874
                     /locus_tag="VF531_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEX7712433.1"
                     /translation="MLIVPVRAAILYLLVVLIVRIMGKHQIGQLQPFELVIAIMISEL
                     AAVPMQDTDIPLINGIIPILTLLFLQVSLSLLSLKSETARRIICGGPSILIENGKIVQ
                     SELTKLRYNLNDLLEQLRLKNYPNISDIEYAILETSGKLSIIPKSQKRPVNPEDLQIQ
                     TPYEGLPITLIVDGSLKRQNLIQAKLTEEWLRNELGKFGLNQWNQVFLASLDTTGKLY
                     IQPNERGRQGA"
BASE COUNT          943 a         1090 c          919 g          987 t
ORIGIN      
        1 ggtatcgggg tcggttagcc gggttacctc gtattccaca gcggcagaag ccgattgctc
       61 acttgacgcg gcggccagat taatctggcg aatcgtatct tccagggact ggcgaagatc
      121 gatggcggca aattcccgga agtatttgga gagtgccgcc tgatcctctt taaacacggc
      181 ttcgctatac ggatgggacc gttccagata ataaataccg aaataatccc tggctccgac
      241 attgcgatag atggtattat aaaggcggta ttcgttcttt tcataatcga tcttcgcgtc
      301 cacccggtaa aggttggcga ggagcttaag taccattcgt tcctggggtg tcatcccgtt
      361 aaactggtag gccatgagaa tctgatattg ataggggtat aggtttcgtt cctccaccag
      421 atcacggtcc gggagatcga aacccgtcgt ttcaggttcg gcgcctatcc ggccgccgta
      481 accgtttccc agtttttcaa gctcaggcaa tacttgccgg ggatcaaaat caccggctaa
      541 aacatatgtt gcctgatctg gctggtaaaa ctggcggtga aactcctcga ggtcttggga
      601 agtgatcgcg gccaccgatg cttgactata actgaactcc gggtaaaaat accggtacac
      661 cggatactcc tggtaacgtt cggaataatc catgtccagt tcgtgaatga caatcttctt
      721 ctcaagctcc aaggcagttc cggaggttat cggcttccac actatgccat tgaagattcg
      781 caacccttcg gaaaggttct ccctgggcac cacaaaatta taagatgtag tgtcaaagga
      841 ggtaaaacca ttgaattgac cgcctttacg ggtagtaacc tccgcaatgt cggtaaagtt
      901 gtaacccgat ccgccccgaa aaaccacgtg ctccaaaaga tgagcgatgc ccttggcctt
      961 ctcgcgattc atcccgatgg ggaaaaccgc atagatcgag accagcggtt cattcttcat
     1021 gaccttgtat aaaacagtca acccgttctt caaagtcgtt cgggtgaagt ggagcccgcc
     1081 cgatgccgcc tgggatgtaa caggccaaac cgtggtcatt cccagccaga tcaagccaag
     1141 cattaacagg tgtaaggggc gcttaccccg gcggttacgc gtatacgcat cgtgcattga
     1201 tgcacgctta atctcggcca tcattgttac ccccaatcac caggcgctcc gggtaagcct
     1261 cccaattcac cgggagcctc cggtttcccg aacgtagctc gatgccctgt ttccgggata
     1321 tggcttcatc tgtttcaaaa taaccgtcat tgttccgatc aatatataat tcatccccgg
     1381 aatcatcgta acgcccgtta tggttccgat caaccaccgc tgcaagcccg gtgccgcctg
     1441 tttcttccag ttttgcaagg aaccaggtcc ggacttcaat ccggaggtaa ggctgatcct
     1501 tgaccaatgg tccgggtcta ttcaaaagcc cgctgaccat gatttgaatc ggtcgacttc
     1561 catcccgatg ggcttgataa ttcacctgaa gagtgatggg atccgggctt tcaacccaat
     1621 aaatctggag cccctgccat tcatcgagca atggaaacca tggggcattg gcgaaactgc
     1681 cgtctccatc ggtatcgaca taaaggcgtt tttcaccgcc gttgatgtcg gcaatcaccc
     1741 cgaacttctg aggctgatcc cccatgacga tcatcccggt aaacggactt ttcatcccca
     1801 aaggcggtaa gtcccgaaat tgttcctgac cccctacggt cagtttgact tcctggagcg
     1861 cgtcccagta ggcttgtcca ttcttgtaat gagtgggagt gtgattggta accgcaatga
     1921 gaggataaga gccgctcacc aaattatccc ccgggagatt ggcggcacta ccaatgccgg
     1981 ctccaagcca aatcccggta acaatcaata aataacgggc aaacttgacc acacgatcac
     2041 ttctttcata cagtggttaa tcccgggcat tcaaagagga cttattttcg gctgaaaccg
     2101 taagtcccca gtgcctcccc cacctggtaa taactgccgt tactataggc aacccccgga
     2161 agcgcgtgta agtccagaac cccccgcgca tcgaggactg tctccgcata atgggcctga
     2221 accatttcgg caataaaaag atcgtgactc ccgagaggta tgacctggct caccttgcat
     2281 tccaaattca ccggggcctc ggcaatgagc ggggcctgaa cctgtttggc cgaaaccggt
     2341 gttaaaccgc aaaccgcaaa cttatcttga tcccttccgg aaaccatccc gcaataatct
     2401 gtcactcgca gcagttcctt gcttggaagg ttgattacaa actccctgct ctggctgatg
     2461 atggcatgag aatacctttc cggccggaca ctgatgctta caatcaccgg atccgagcat
     2521 agcaggccag tccaggcgat ggtgatgata ttggggcgat attccggcgc cgcgcagctg
     2581 accatgacaa ccggaaccgg cgctaaaacc gtgccccctt gtttcgtaat ctttctcatt
     2641 gggaatcccc gccttcgctg ccggtatcct gcggtagttc gatcccaacc gcatcggcga
     2701 tggttctctg gattaccatc attaattggc tgaattgata ctctgccatt agatattccc
     2761 gaacgtaggg atttagtcca acgatctccg ccagtttttt tagctccgtt tcgtacggtt
     2821 cgagcagctt ttcgccgctc aatctctttc gttccagctc caattgcttt ttccggaaat
     2881 cctcgaacat ggacttggct gcctcatgtt catccagtac ttgcttggct gcccgatatt
     2941 tacggtattc ctcgctttct gccaacgagg cggctaattg tttcgccaat tcctgaggtt
     3001 tcatcctgat tcctccctgt cgaaagccta ttgctgatca ttaattcgtg aattcgtgtt
     3061 tcagcctaaa aatcctgcca gtaattccac tttctacttt gcctatcatt cccggtccaa
     3121 gcataaactt ttccctcttt gcaaaaaatc agatctagac ttgagttcct tatgaggtga
     3181 aaccatgcta atcgtgccgg tccgcgccgc cattctttat ctattggtgg ttttaatcgt
     3241 ccgcattatg ggaaaacatc aaatcgggca attacaacca tttgaactgg tgatcgccat
     3301 catgatctcc gagttggcgg cggttcccat gcaagacacc gatatcccgc tgattaacgg
     3361 aatcattcct atactcaccc tgctgtttct ccaggtcagt ctgtccctgc tttccctaaa
     3421 aagtgagacc gcccgcagga tcatctgcgg cggacccagc atcctcattg aaaacggcaa
     3481 gatcgtccag tcggaactga ctaaactccg ctataacctg aatgacctct tggagcagct
     3541 tcgcttgaaa aactatccca acatctccga tatcgaatac gccatccttg agaccagcgg
     3601 caagctctcg attattccca agtcccaaaa acgaccggtc aatccggaag acctccagat
     3661 tcaaacaccc tatgaagggc tgccgatcac actgattgtg gacgggagtt tgaaacgaca
     3721 aaatctcatc caggctaagc tcaccgagga atggctccgg aatgagctgg ggaaattcgg
     3781 acttaaccaa tggaatcaag tcttcctggc cagtctggat acgaccggaa aactgtatat
     3841 tcagccgaac gagcggggga ggcagggagc ttgaaagtct ggctatattc cttacttggg
     3901 ttggccatct ttattggctg cgccctgatg gttcaatgg
//
LOCUS       DASVMB010000011         7136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_517079,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000011 DASVMB010000000
VERSION     DASVMB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517079"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VF531_00305"
     CDS             complement(<1..1015)
                     /locus_tag="VF531_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7712434.1"
                     /translation="MRKTQLLTIFLLIFALVFLNMASSAASRSFSNVPLIAAIENPDS
                     TTVLPESAATRESDTVTTTPEPAETNSEPGQISAEAVPASRTSRLKPAPVPTPGPQDP
                     AIQTNNLSSPSSPVTTPEPRLRTEARPARFEINIRTIGQWLSSWWNRTDFHLQLWYIL
                     CIIGLLLYLLRWNRLRQPFLFLCLILLGFYLGGPPDPVNAVPGLFIPGAGWWTALILL
                     CIPVVISFLWGRVFCGWICPLGAVQEFLHIDSSKRQLPAGLDRFLKYVKYLILIALGY
                     LTWRTGQNYWSNYEPSASLFTFNGSAVALMILGGFLLVSVLISRPFCRYICPLGAILA
                     ITSR"
     gene            complement(1113..1262)
                     /locus_tag="VF531_00310"
     CDS             complement(1113..1262)
                     /locus_tag="VF531_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712435.1"
                     /translation="MTNTDQINLELIVNLLGPEFLEDHPFLTRRIPPFPDTRSSLNLQ
                     AHNSY"
     gene            1747..2697
                     /gene="spoIID"
                     /locus_tag="VF531_00315"
     CDS             1747..2697
                     /gene="spoIID"
                     /locus_tag="VF531_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HEX7712436.1"
                     /translation="MRSNGVMAIFLLFTLLVLIPVLYLKRGFPAERVPSSSGISISLY
                     ARQEGRVYRLDFEDYLIGVVAAEMPAEFGVEALKAQAVAARTLAIRRLPRFGGRGCQH
                     TRGADFCDDPGENQAWLSNDELYRKWGRSEFETYYRKIRRAVQETRGVVMVYNNRLID
                     AVFHSTCGVGTAAAREIWHYDIPYLQNESCGFDQESTRLKTSYHFSWSRLAQLFGFPE
                     TTVRRLTVGDRFSDGRISWIAMGGNRVTNEDFRIKLGLNSTYFNWKRSGDGIQLTTVG
                     YGHGVGLCQYGAGGMAKQGWKYGRILSHYYRGIQFRRIRY"
     gene            2755..3489
                     /locus_tag="VF531_00320"
     CDS             2755..3489
                     /locus_tag="VF531_00320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7712437.1"
                     /translation="MVKRTNHNEAWRKRCHRISQVLKGSWRNFSEKVRPYLAMKYWPI
                     YLIAFALGFYLWGPGHGIRKIQAWSFPGVARKTDQKSIGNLQQELNRLKRELAQEKGK
                     SSTVVFDPTSFSRPALGEVIQGYDWVYSEKSWRFHPGVDIITSGNPNVLAAAAGMVTK
                     VQKTANGDMTVTLDHGSGWTSYYAGLSGIQVSEGQRITKGVILGASGNLNCLPGESGR
                     IGFHFAIYRDQQPLDPRNIIKGLVKI"
     gene            3630..3926
                     /gene="spoIIID"
                     /locus_tag="VF531_00325"
     CDS             3630..3926
                     /gene="spoIIID"
                     /locus_tag="VF531_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HEX7712438.1"
                     /translation="MKDYIQKRVLDLSHYIADRKATVRQAAKFFGVSKSTVHKDVTER
                     LPDINRSLSREVKRILQINKAERHIRGGEATKRKYQIREIPAKTLKKGGYSKKT"
     gene            3976..5010
                     /locus_tag="VF531_00330"
     CDS             3976..5010
                     /locus_tag="VF531_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7712439.1"
                     /translation="MNWNNLVTDIGIDLGTSSVLVYVKRRGVVIREPSVVALQKDNNK
                     ILAVGEEARQMIGRTPGNIVAIRPLKDGVIADYDVTETMLKYFIEKVCPKPRLFRPQV
                     VACVPSGVTSVEKRAVLEAAMQAGAKRTYLVEEPMAAAIGAGLNITEASGNMVVDIGG
                     GTTDVAIISLGGIVVSESLRVGGDKFDEAIIRYIKKMYNMMVGERTAEDIKIQIGSAY
                     PEGEGKSMEIRGRDLVTGLPRTIKFTSTDSFQALSEPITTIIDGIKSVLERTPPELAS
                     DIVDKGIVMTGGGSLLHGFSKLLSEETGIPIHLAEDPLSCVALGTGKILENEGFEAIR
                     HSLIVGSRSM"
     gene            5130..5924
                     /locus_tag="VF531_00335"
     CDS             5130..5924
                     /locus_tag="VF531_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HEX7712440.1"
                     /translation="MDLSRLSLPRTYTRKLLRAIREFELIQPGDRVLVGFSGGKDSSF
                     LIYALAIFKEHQIIPFDLAALTIDLGFETPMDLTRMQAFCDGLEVPLHVVKTEIAKYA
                     FSEDNPEGPCATCSFLRRGTMNRFAREHGFNRVALAHHHDDAVETFLMSIIYSGQIKT
                     FLPRTDLERTGLTVIRPLVYFREAELRKAIRLTGFEPIPAPCPADGQTKRAETKDMIR
                     KLCRQNKRVFNNLAAVIREGRPIELWPAELSQEEKRRRNQPNPPNP"
     gene            6053..6754
                     /locus_tag="VF531_00340"
     CDS             6053..6754
                     /locus_tag="VF531_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX7712441.1"
                     /translation="MIPQDKITASLDALYEKIDFEGREAIRNRREMIDPVLNHLEQDK
                     RLGLTLLIHIGGTVAGSLKAFMDEMRKLEPDQYFYPESDLHVTVLDLISADQNFERNR
                     LVIDESLELAKSAISGLPPFCIDFNGIITSNAAVLAKGYYTGGLRNLRDRLRSIAMEQ
                     GFDLRERYQSISAHSTIIRYKSALQNREKFLTTLEKYKRFNIGGLKVNELELVIHDWY
                     NRRREAIGKFYLGQV"
BASE COUNT         1819 a         1608 c         1975 g         1734 t
ORIGIN      
        1 ttcttgaggt gattgcaagg atcgcaccga gcggacagat gtaacggcag aacggccggg
       61 agatcaagac agataccagg aggaatccac ccaggatcat caaggctacg gctgagccgt
      121 taaacgtaaa taggctcgcg gagggctcgt agttgctcca ataattttga ccggttcgcc
      181 aagtcagata accaagggcg atcaggatca gatattttac gtatttaagg aatcgatcca
      241 gccccgccgg taactgacgt ttggaggaat cgatatggag gaattcttga accgccccaa
      301 ggggacagat ccaaccgcag aaaaccctgc cccataagaa ggagatcacg accggtatgc
      361 acagcaggat aagggcggtc caccacccgg cacccggaat gaacaggccg gggacggcgt
      421 tgaccgggtc ggggggccca cccaggtaaa aaccgagcag aatcaggcat aaaaagagaa
      481 agggttggcg taagcggttc cagcgcaaga gatacagcaa cagtccaatg atgcatagaa
      541 tataccaaag ctggagatga aaatccgtcc ggttccacca ggatgagagc cattgcccta
      601 tcgttcggat attgatctcg aaacgcgcgg gacgtgcttc ggtccggaga cgcggctccg
      661 gggtggtgac aggtgaagaa ggcgacgaaa gattattcgt ttggattgcc gggtcctgcg
      721 gtccgggggt tggaacggga gccggcttta acctggatgt acgactggcc gggactgcct
      781 ccgccgaaat ctgtcccggt tccgaatttg tttccgccgg ttccggagtt gtggtgactg
      841 tatcactctc ccgggtagcg gctgattcag gtaagacagt ggtagaatcc gggttttcta
      901 tggctgcaat caaagggaca tttgaaaaac tccggctggc ggcgctagag gccatattta
      961 agaatactaa agcaaagatc aatagaaaga tggtcagcag ctgagttttg cgcattagat
     1021 cacctctaca aaatatttcg gcggcgatcc ttaattttcc ttctacaaag aatggaaaaa
     1081 accaaacccg acggctgtgg agtcaaaacc gatcaatagc tgttatgagc ctggaggttc
     1141 agactggatc gggtatcggg aaatggcggg atccttcggg tcaggaacgg atgatcttcc
     1201 aaaaactcgg gacccaatag attgacgatc agttccagat tgatttgatc cgtgtttgtc
     1261 atcgttcact ttctccttac aagttcaatc aacggatgag aacgtttagc cgggagtttt
     1321 gatgggtggt tcggatcttc cattttcagc aagctccttg acggacgtct catttttttc
     1381 ttcggcggga atgacgagaa acctgacggg tttttcaaat tgttaacgta agctttgtca
     1441 tagacaatta taaatttcca agttcatatt taactattgc cgttcggcat cccatccccg
     1501 ttcccaacgg gcctgatgcg tcttggctac gggtttagtg aatttaggga tcaggttcgg
     1561 ttaaaaatac gcggatgcct gtccggggta agcggattgg agccgttcat gggtccgtgc
     1621 atcttttcaa tttctcccgc ataaggtgaa ctattcctcc gctgaatttg cggggttggc
     1681 gggaaatcat cggagggcgg gtttaactgc tgtcattcgg tttgcgacgg cccgggaggg
     1741 atttttttgc gatccaatgg cgtcatggcg atttttttgt tatttacgct gctggtactc
     1801 atcccggtgc tttatttaaa gcggggattt cctgcggagc gggtgccatc atcatccggg
     1861 atttccatat cattatatgc gcgtcaggaa ggccgggttt atcgtcttga ttttgaggac
     1921 tacctgatcg gagtggtggc ggctgagatg ccggccgagt ttggtgtaga agctttgaag
     1981 gctcaagcgg tggcggcgcg taccttggct atccggcggt taccccgttt cggaggtagg
     2041 ggttgccaac atacccgggg ggccgacttt tgcgatgacc ctggtgaaaa tcaggcctgg
     2101 ctttctaatg acgaattgta tcggaaatgg gggcgaagcg agtttgagac ctattatcgt
     2161 aaaattcgcc gcgcggttca ggaaacaagg ggagttgtca tggtctataa caaccggctg
     2221 atcgatgcgg tctttcattc aacctgcggt gtgggtactg ctgcggcccg tgagatctgg
     2281 cattacgata tcccctactt gcagaatgag agttgtggtt ttgatcagga atcgacccgc
     2341 ttgaaaacca gctatcattt tagttggtcc cgattggctc aactttttgg ttttccggaa
     2401 accaccgtcc ggcggctcac ggtaggggac cgtttctcgg atgggcggat cagctggatc
     2461 gccatggggg ggaaccgggt aaccaacgaa gactttcgta tcaaactcgg ccttaattca
     2521 acctatttca actggaagcg gagcggtgac gggattcaac tgactaccgt cggttacggc
     2581 cacggcgtgg gcttatgcca atacggagcc ggcggcatgg ccaaacaggg ttggaagtat
     2641 gggcggattt tatcccatta ttaccggggg atacaattcc gccggattcg ttattgacgg
     2701 ataaaatttc ctttattgtg ccaaactatc atcagttgta aaaggggtga tagtatggta
     2761 aaaagaacca accataacga ggcatggcgg aaacggtgtc acagaatttc tcaagtgtta
     2821 aaaggatctt ggcgaaattt ttctgagaaa gtccggccgt atttagccat gaaatactgg
     2881 ccaatctatc tcattgcatt tgccctgggg ttctatttat ggggtcccgg tcatggtatc
     2941 cgcaagattc aagcatggtc gtttccgggt gtggcccgta aaaccgatca gaagtccatc
     3001 ggcaatttac aacaggaatt gaatcgcctc aagcgggagt tggctcagga gaaaggcaaa
     3061 agcagcaccg tcgtatttga ccccacgagt ttcagtcgcc cggccttggg tgaggtgatc
     3121 cagggatacg attgggtata ctccgaaaaa agctggcggt ttcaccccgg agtggatatc
     3181 attacgtccg ggaacccgaa tgtgctggca gccgcggccg ggatggtcac taaggtgcaa
     3241 aaaacggcga atggcgatat gacagtcaca ctggatcacg gaagcggctg gacaagttat
     3301 tatgcggggt tatccgggat tcaagtcagc gaaggtcaaa ggattaccaa aggggtcatc
     3361 ttgggagcca gcggtaactt gaattgcctc cccggggagt cggggcggat cggttttcat
     3421 ttcgccattt accgggacca gcagcccctc gatccccgga atatcattaa agggctggtg
     3481 aagatataag aagatacggg tagggcgtat ccggatgatc cattttaaaa tacaacgtat
     3541 cttccgatag gccgtttcta agacatacga catattcccc tccacccacc aatatacatt
     3601 taataaaaaa ccacgggagg gtggggggag tgaaagacta catccaaaaa cgagtcctgg
     3661 atctcagcca ttatatagcc gaccgtaaag ctaccgtaag acaggccgcc aaatttttcg
     3721 gcgtcagtaa gagtacagtc cacaaggatg ttacggaaag gctgccggat atcaaccgaa
     3781 gtttatcacg cgaagttaaa cgaatacttc agataaataa ggctgaacga catattcggg
     3841 gtggcgaagc cacgaaacgt aagtatcaaa tccgggaaat acccgcaaaa actttaaaaa
     3901 aaggaggata ttccaaaaag acgtagaaga tttacacatc ttaatggtat taggtgatta
     3961 ggggggcagc ttcttttgaa ctggaataac ttagtaacgg acatcggaat cgatctcggg
     4021 acatcaagtg ttctggttta cgtaaagaga cgtggtgttg tgattcgaga gccctccgtt
     4081 gtcgccttgc agaaggataa taataagatt ctagcggtgg gtgaggaagc ccggcagatg
     4141 attgggcgaa cccccggtaa tattgtggcg attcgccctt taaaggacgg cgttatcgcc
     4201 gattatgacg taacagaaac gatgttgaaa tactttatcg agaaagtctg ccccaaaccc
     4261 cggttgttca ggcctcaggt agtggcttgc gttccttcgg gagtaaccag cgtcgaaaag
     4321 agagcggtcc tggaggcggc gatgcaagcg ggcgccaaac ggacctattt ggttgaggaa
     4381 cctatggcag cggcgattgg cgccggcctt aatatcacgg aagccagcgg caacatggtg
     4441 gttgacatcg gcggtggcac tacggatgtg gccatcatct ctctgggcgg aatcgtggtc
     4501 agcgaatcgt tacgggttgg cggggataag tttgatgaag ccatcatccg ctacataaag
     4561 aagatgtata atatgatggt cggggaacgg accgctgagg atattaagat tcagatcggt
     4621 tccgcatatc ccgaagggga aggaaaatcc atggagattc ggggccgcga tctggttacc
     4681 ggcctaccgc gaacgatcaa atttacctct accgattctt tccaagcttt atccgaacct
     4741 atcactacca tcattgatgg tatcaaatcc gtgcttgagc gaaccccacc cgagctggcc
     4801 tcggatatcg tggataaagg aatcgtaatg actggcggcg gatccctgct gcacggcttc
     4861 tccaagctgc tctcggagga aacagggatt ccgattcact tggcggaaga ccccttgtcc
     4921 tgcgtggcct tggggacagg caagattttg gagaacgaag gttttgaggc catccgccac
     4981 agcttaattg taggaagccg ctcgatgtaa gcgatataac caaaataccg gtatctggag
     5041 atcaaccaat ccggctttat ttttttctgt ttcaaattcc ggtccggaaa tcgtggccgg
     5101 aatttattat gtcgggcgaa ggagcatcga tggatctttc tcgtctcagc ctgccccgaa
     5161 cttacaccag aaaactactt cgggcaatcc gggaattcga actgattcaa cccggggatc
     5221 gggttttggt gggattttcc ggtggtaagg acagttcttt tctcatatac gccctggcca
     5281 tctttaaaga gcatcagatc atcccttttg atctggctgc cttaaccatc gacttgggat
     5341 ttgaaacccc gatggatctc acccggatgc aggcattttg cgacggactt gaagtcccgc
     5401 ttcatgtggt aaagacggag atcgccaaat acgcctttag tgaggataat cctgagggac
     5461 cctgcgccac ctgttccttt ctacgccgcg gcaccatgaa ccgatttgcg cgggagcatg
     5521 gatttaaccg ggtggcgctg gcgcatcatc atgacgacgc cgtggagacc tttttgatga
     5581 gcatcatcta ttcggggcag atcaagactt ttttgccccg gaccgatctg gagcgaaccg
     5641 gattgacagt catccggccg ctggtctatt ttcgtgaggc cgaactgcgc aaggcgatcc
     5701 ggttgaccgg gtttgagccg attcccgcgc catgcccggc cgacggccaa accaagcgtg
     5761 ccgagacgaa ggatatgatt cgtaaactct gccgccaaaa taaacgggtc ttcaataatc
     5821 tggccgcagt gatccgggag gggcgcccca tcgaactttg gccggccgag ctgtcacagg
     5881 aagagaaacg gagaaggaat caacctaacc cacctaaccc ctagtccctt ccctcacatt
     5941 tcgggtctag atccgggaca gggaagggga actatatagt tatcagagga tttttttatt
     6001 aaagtattag tgatgcggat atagcggcac agatatgaga aggtagataa gtatgattcc
     6061 gcaggataag ataacagcca gtttagatgc attatacgaa aaaatcgact tcgagggcag
     6121 ggaagccatt cgtaaccggc gggaaatgat cgatccggtc ttaaaccatc tcgaacagga
     6181 caaaagactg gggttgacgc tgttaattca tatcggagga acggtggccg gaagtcttaa
     6241 ggcgttcatg gatgaaatgc gcaagctaga gccggatcaa tatttttatc cggaatcgga
     6301 tctccatgtg acggtgctcg atctgataag cgcggatcag aattttgaaa gaaatcggct
     6361 tgtgatagat gaatccttag agctggcgaa gagcgctata tccggtttac cgccgttttg
     6421 tatcgatttc aacggaatca ttaccagcaa tgcggcagtt ttagccaaag ggtattatac
     6481 cggtggattg cggaatttac gggatcggtt acgaagtatt gctatggagc aaggctttga
     6541 tttgcgggaa aggtatcaga gtatttcggc tcattcaacc atcatccggt ataagtcggc
     6601 cctgcaaaac agggagaagt tcttgactac ccttgaaaag tataagcgat tcaatatcgg
     6661 cgggctgaaa gtcaacgaat tggaactggt gattcatgat tggtataaca ggcgtcggga
     6721 ggcaatcggg aaattttatt tagggcaagt atagagttaa ctttgttttt tcaagttgac
     6781 aaaataccta aggcaggtgc agcatgccaa tgctgctggg gagcggggtg tccccgtata
     6841 aaattagaca aaattcccct cctcgaggag ggctattcat tacccataag taacattaat
     6901 gactataaaa atacgaattt cccgaaatta ttggtgtata atttgttcgg atattttgcc
     6961 aacccgcggg gtgaatcgcg ttcgaagaac acgcgcccac ggctgttaca aatcaacccc
     7021 cataaatggg gctcttttgg gcctaaccta attttttggt tatgatgttg cattttcttc
     7081 gagtttagcc ggcttcagcc gggttggttg tatgcatagc cgtgtgggtt tacccc
//
LOCUS       DASVMB010000012         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_1948,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000012 DASVMB010000000
VERSION     DASVMB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1052)
                     /locus_tag="VF531_00345"
     CDS             complement(159..1052)
                     /locus_tag="VF531_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7712442.1"
                     /translation="MKYGVFSVTTPEYNLKETVTVLKELGYDGVEWRVSNPVPEKKPT
                     DYTYERRYWSYNMSTLDINRIEEDALEAKNLCDRAGIEIYSLTTYLKPADIQTVERVL
                     KSAAEIQCKNIRIFPPNYDETENYRQLFERTIAELKRVEALAAQYGVRVNLEIHMGNI
                     IPSASAAYRLVSNFDPRYIGVIHDAGNMVFEGFENYRMGLELLGPYLAYVHVKNAVWI
                     PDKTGIDGTESGAWARWKPVWAPYQKGFANLTKLLSVLKDRGYRGYISVEDFSNEVET
                     YAKLRENLQFLKSIEGGNVND"
     gene            1182..2021
                     /locus_tag="VF531_00350"
     CDS             1182..2021
                     /locus_tag="VF531_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7712443.1"
                     /translation="MLNSYYADADHWLKFTHTRSFPLDTIEFHLHNYFEIYFFIAGDV
                     DYFIEKRVHLLKYGDLLLMNNHEIHKPTFRSQEAYERIVIHFEPELGRLLSAPGFNLL
                     DCFLNRPKGELNKIKLSPSEVEELMGIFKRLETLTPHPPAGSETLRLSYLIEILVFIN
                     QVFLNIKPAADHGGLPEALIPILEYIDNHLDEDLTLDSLGHRFFMNQSHLSRLFKKGI
                     GSNIHEFIIYKRISKAKKLLSEGYNVTDTCSRCGFNDYANFLRMFKRKVGVSPGRYKG
                     GKK"
     gene            2018..2191
                     /locus_tag="VF531_00355"
     CDS             2018..2191
                     /locus_tag="VF531_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7712444.1"
                     /translation="MGLWEEAYETVVKSGYEIIHPFEKQAWGQNVFRFYDPDRHIPEI
                     GEATHLIDDDFCY"
     gene            2239..3003
                     /locus_tag="VF531_00360"
     CDS             2239..3003
                     /locus_tag="VF531_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7712445.1"
                     /translation="MEYTVHKLSKLAGVSTRTLRYYDEIGLLAPAKINTSGYRLYGRD
                     EVDRLQQILFYRELGVGLEKIKQIITAPSFDRNNALREHREQLLEKRRQLDLLIATID
                     KTIATTEGRITMSDQEKFEGFKQNLIDENERKYGTEIRTKYGNEVVDKSNAKVKGMSQ
                     ADYEEVTKLAQEILDTLKAAMKTGDPAGPLAQKAADLHRRWLSFYWDGYTKEAHAGVA
                     QMYVDDERFKAYYDRNQPGMAEFLRDAVLVYTGTKK"
BASE COUNT          887 a          789 c          745 g          869 t
ORIGIN      
        1 gttttttgat gtccctcgct tcatccaagg tcaactcgct tacattcttg ccgttgacac
       61 cgcgcatctc aatataccgg ataccgtact catccaaaac atccatctgg gtctgtaaac
      121 tatcatcgat ctcatcggca aacgcgctta atatgaattt agtcattgac attgcctccc
      181 tcaatactct tcagaaattg aaggttttcc cggagtttgg cgtaggtctc cacttcattg
      241 gagaagtcct cgactgaaat atatcctcga taccccctat ccttcaacac cgaaagcaac
      301 ttggtaaggt tcgcaaaacc cttttgatac ggcgcccaca ccggcttcca tcgcgcccat
      361 gcgcccgact ctgtcccgtc aatacctgtt ttatcaggaa tccaaacggc attcttgaca
      421 tgaacgtagg ctaagtacgg accgagtaat tccagaccca tccggtagtt ttcgaagcct
      481 tcaaagacca tatttcctgc atcatggata accccgatat accgcggatc aaaattggag
      541 accaaccgat acgccgcgct ggcactgggg atgatgtttc ccatgtggat ttccagattg
      601 acacgcactc catactgcgc ggccagagct tctaccctct tcagctcggc aatggtccgt
      661 tcaaacaact ggcggtaatt ttcggtttca tcgtaattcg gcggaaatat ccggatgttt
      721 ttgcattgga tttcggctgc ggactttagc acccgctcca cggtttgaat atccgccggt
      781 tttagatagg tggtcaagga ataaatctcg atccccgccc ggtcgcagag gtttttagct
      841 tcaagcgcat cctcctcaat ccggttaata tccaaggtgc tcatattata cgaccaatag
      901 cggcgctcgt aagtataatc cgtaggtttc ttttccggga cagggttact aacccgccac
      961 tccaccccgt cataaccgag ctccttaagg accgttacgg tctctttgag gttgtattcc
     1021 ggggtggtga ctgaaaaaac cccatatttc atctgcttca ctcctttttt gcgaatatcg
     1081 tcgcgtccat ctttatttta gtgtatgata aaaacataat cattgcatat tttgccataa
     1141 atattgcctt ttttgcaatg cttttgatgg aggatacttc catgctcaac tcctattacg
     1201 ctgatgccga tcattggttg aaattcaccc atacccggtc gtttccattg gataccatcg
     1261 aatttcacct gcacaactat tttgaaatct acttcttcat cgccggcgat gtcgattact
     1321 ttatcgagaa gcgggttcac cttttaaaat atggcgattt gctgctcatg aacaaccacg
     1381 aaatccataa accgactttt cgctctcaag aagcatatga acggatcgta attcattttg
     1441 aaccggagtt aggccgcctc ttaagcgctc cgggttttaa cctgttggat tgctttctca
     1501 atcgtcccaa gggagaattg aacaagataa agctcagccc atccgaagtt gaagaattaa
     1561 tggggatctt taaacggttg gaaacactta ccccccatcc gcccgccggt tctgagactc
     1621 taaggctgag ttacctcata gaaatcttgg tctttatcaa ccaagttttt ttgaatatta
     1681 aacccgccgc tgatcacggc ggtcttcccg aagcgctcat cccgatcctg gaatacatcg
     1741 ataaccatct ggatgaagac ttgactttag attctttagg tcacaggttc tttatgaatc
     1801 aatcccattt gagccgcctc tttaaaaaag ggatcggctc caatattcat gagttcatta
     1861 tctacaaacg catctccaag gccaagaaat tgctgagcga aggatataac gtcaccgata
     1921 cttgttcaag gtgcggattt aatgattatg ccaatttcct gcggatgttc aagcgcaaag
     1981 tgggggtgtc tccggggaga tataagggcg ggaagaaata ggtttatggg aggaagctta
     2041 tgaaaccgtg gtaaaatccg gttatgaaat catccatccc ttcgaaaaac aggcctgggg
     2101 tcagaatgtc ttccggttct acgaccccga tcggcacatc ccggagatcg gggaagccac
     2161 gcatctgata gacgatgatt tttgctattg acattgacgt tacgtaaggg tttatattta
     2221 tctcaaggag cgcgaaacat ggagtacaca gtgcataaac tgagtaagct ggcgggtgtt
     2281 agtacccgga ctctgagata ctacgatgag attggcttgc tggcgccagc gaagatcaat
     2341 acatcggggt accgcctcta cggccgggat gaagtcgacc ggttgcagca gatcctgttc
     2401 tatcgggagc tgggagtagg cttggaaaag atcaagcaga tcatcaccgc gccttccttt
     2461 gaccggaata acgcactgcg agaacaccgt gagcaactcc ttgaaaaaag aaggcagttg
     2521 gatctgttga tcgccactat cgacaagacc attgccacaa cggaagggag aattaccatg
     2581 tctgatcaag aaaaattcga aggttttaaa caaaacctaa tcgatgaaaa tgaaaggaag
     2641 tacggcacgg aaattaggac caaatacggt aatgaggttg ttgataagtc caacgccaaa
     2701 gtgaaaggaa tgtcccaggc cgattatgaa gaagtaacca agcttgcaca agaaattctt
     2761 gacacactga aggctgccat gaaaaccggc gatcccgctg gcccgctggc ccagaaagct
     2821 gcggatttac accgccgttg gttaagcttc tattgggatg gttacaccaa ggaagcccat
     2881 gcgggggttg cccaaatgta tgttgatgat gagagattca aggcttacta tgatcgaaat
     2941 caacccggaa tggcggagtt tctgagagac gcggtgttgg tgtataccgg aaccaaaaaa
     3001 taaccatcat gtttgatcat ctcccaaagg attactctaa agtgggagta aataatgtga
     3061 ccccttcgtt tggcggatag cccaaaaatc ccatgacgaa gggttttttt gcacctttca
     3121 cgccatagcc attgaatgaa ttccgatatt accggtaata ctttgattac cagggaatgg
     3181 atagcacttt tgatgcaaaa ttttgaggag tatgagcgga aagatggacg gccccctgac
     3241 gggaaccagc ttaagggaga ctttagcaag gatcagggat tatcttggca
//
LOCUS       DASVMB010000013        17913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_2137,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000013 DASVMB010000000
VERSION     DASVMB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 17913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17913
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF531_00365"
     CDS             complement(<1..355)
                     /locus_tag="VF531_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712446.1"
                     /translation="MAKVKSQAAVSADIDSLIHKKKKRRMKPEWIWGYLFIAPIFIGI
                     VGFHIGPIIYSFLMSFTRWDSLTQPVFIGLKNYLDLFIDQQVGHEFINMMYYTIGVVP
                     LTIFLSLILANALNQK"
     gene            complement(494..1744)
                     /locus_tag="VF531_00370"
     CDS             complement(494..1744)
                     /locus_tag="VF531_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7712447.1"
                     /translation="MKRIVIVLSAFVALMFMLTFTGYGSSKNVVLNFYYWDENQKPAM
                     DQIIKDFEKRNPGIAIKSTVIPWDQYWMKIQTSLPTSSGPDILWLNGINAPKYMSVGL
                     ILNLQKYIDKEKTDLSNFPDSLKKIYSLNGKLYAIPKDYDTIALLYNKQCFDEAKVPY
                     PTDDWTWDDLLAAAQKLTLKNEDGSVKQWGYYCLPSIQGGVADAIYQNAGQIYNKDLT
                     KAVINSPENVETVQFLVDLMYKYKVSPNGAQQKDIDYLKAFSSGQVAMYNDGSWMIND
                     YSKVLGDKLGVAALPYRKQRATIIHGLGFAASAKTKYPNQAWKFLKFLATKEAGLYQA
                     KAVIPAYKGAETQWIQNFPAVSNVKVFIDETKYAFPLPVSSKNSAAVWSALDNELQNI
                     WLKTKDVATGLADAQKAMNNEMNK"
     gene            complement(1836..3128)
                     /locus_tag="VF531_00375"
     CDS             complement(1836..3128)
                     /locus_tag="VF531_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7712448.1"
                     /translation="MKKTYFWLMALVMLVTMGGFIGAAGKEVTLRYARWGIPDELSGT
                     KVMLNAFMKANPNIKVTLEASSWDTYWEKLQTQVASDEVADVFLFDGGWYLNQFAPKG
                     VLMDLAPLMKRDGIKKTDYYNVWDTFTYKNKIYSIPRDYNAIVMFYNKDLFAKAGIKD
                     YPSGNMSWDQVIELAKKLTLDNKGRNANDPGFDPDNIDQYGLFVATADIDQVLETFTF
                     QSGGQLISPDGQKFFIDSPKVKKVIQFLYDLTWKYHVKPTPAAVAKFQDVYFQTGKFA
                     MVYQGSWQVSQLNGVTFDWDVAVAPTFGKKIYCVQSVGNSMYAKTKHPKEAWKLIRFL
                     CGKEGQTIMAKEGDAIPVLKEVAMSPAFLNPSQKPLNKKAYFESAVGIVPYISFPKKG
                     EIFERINQDLESAYNNKKTVEAAIKACKQDVERIINLD"
     gene            complement(3182..4048)
                     /locus_tag="VF531_00380"
     CDS             complement(3182..4048)
                     /locus_tag="VF531_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712449.1"
                     /translation="MNTVAKNRPGFISKTTLIKVIQCLFLIAGGILFMAPLIWLVSNS
                     FKENAQIWTYPPVWIPKPVVFKHYLRLLDHHYLPMDTFIINSFKVTIGVLIGNIFSTV
                     LVSYAFARLKCKLKNFYFIVLLGTMMIPGQVILIPVFILYKYLHWLNTFYALIVPAFF
                     GNAFFIFLMRQFMMTIPYELDESALIDGASNLQILTRIILPLCKPILITVGIFSFIWT
                     WTDFYGPLIFLTKMDKMTLAVGITLLQGQRVAEVGFMSAATVISVLPLIILFFSAQKY
                     FVEGITLTGIKG"
     gene            complement(4052..4981)
                     /locus_tag="VF531_00385"
     CDS             complement(4052..4981)
                     /locus_tag="VF531_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712450.1"
                     /translation="MGKDAKANFSKQIFYKLRHKESLKFYLFASPWLIGVLVFFIGPA
                     LISLYLSLTKYDIISSPEFIGLKNYRFLFTMDDLFYKSLANTLYMVFIALPIGIIAQI
                     SLSLFMNLRLKGIAFFRTIYYLPVLVPAVSSAILWRIMLDDRFGLINVFLRFCHIPPQ
                     EWLNNPVLSKPSLILMGLWTIGDGMLIYLANLKDIPGSLYESAAIDGANAWHKFWAIT
                     LPMITPTIFFNLIMGVINTFQTFTSAYVMTAGGPEYSTYFYMYYLYQTAFKFGRMGMA
                     SAMAWILFLVILVLTVIILRSSKHWVFYEGERK"
     gene            complement(5031..6356)
                     /gene="melA"
                     /locus_tag="VF531_00390"
     CDS             complement(5031..6356)
                     /gene="melA"
                     /locus_tag="VF531_00390"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX7712451.1"
                     /translation="MAKKITFIGAGSFGFTRELVRDILTFPALADSTIALMDINDERL
                     AFAKAAVEKIIAAGNYPAKVVATKDRAEALKGADGVLCTILAGGVNVWRHDIEIPKKY
                     GVDICVGDTRGPAGVFRALRTIPVMLDIVKDIERYCPDAVFLNYTNPMAMLCRAMQGQ
                     SKIKVTGLCHSVQGTAEMLAEWIYAPMNEITYLCAGINHMAWYLDYRWRGQDAYPLIK
                     KALEKPEIYHQEMVRNEMFRHFGYYSTESSGHNSEYNPWFRKRPDLIEKFCTHGTGWN
                     PGHYAYILQEYLDADTTWKNKTQEWLDSKEPLDLQRGEEYAAYILNATIGDGELYKFN
                     GNVRNFGLIDNLPEGCCVEVPVLASRRGFEPMHVGPLPDNVAIITNTAARCEELAVQG
                     SISGERDLIYQSIYFDPLTAAVLSLDEIKKMVDEMFAVNQEWLPQFYKH"
     gene            complement(6414..7454)
                     /locus_tag="VF531_00395"
     CDS             complement(6414..7454)
                     /locus_tag="VF531_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7712452.1"
                     /translation="MDHTYTEIKSQYSALRKTFNYILSQKENLLRFYKDQAPRSLTYI
                     GCGSSFYLCQSAEFSARLRLSLPATAMAAGDLMLNYEHYLKVLEWTLVITPTRSGSTS
                     EVLMAIQNVRAVQNVPVLAITCVAGSALSKLADFTLELPWAFDESVCQTRTVTNLYTA
                     NLLIIAILSGDERLIASIDAAIDGGTAYMERYESDLAQIAKENWREAVVLADGEIQGI
                     AREGALAFTEIARVPGQYYHLLDVRHGPMVLIRDKTLVVMALNPGDLRFQKDLINDLL
                     QKGATVIVYSDTPMPLIPGVSLQVALGANLDFAARGIPFILLLQMLSYSKAIQNGVDP
                     DQPEGLDAWIKL"
     gene            complement(7586..8302)
                     /locus_tag="VF531_00400"
     CDS             complement(7586..8302)
                     /locus_tag="VF531_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7712453.1"
                     /translation="MLDEKAPVTLYHQLKEILVGKIKSNEWGIDTKIPTERELCDLYK
                     VSRITVRQALEELEDEGFLYRKQGKGTFVTAPKIEQRLSNFYSFSEEIRNMGFTPSTE
                     MIDFKILTLDEVVAKHFNENAMTVYSIKRLRLANHEPFALETSYIPCDLCPGMTAAEI
                     GTNGLYNTMKRKYNLVPDQAIETFEAVIINPNNAAHLRVGKNSPGILLERTTYAGERI
                     VEFCHGIIRGDRYKYQVVLK"
     gene            complement(8476..9108)
                     /locus_tag="VF531_00405"
     CDS             complement(8476..9108)
                     /locus_tag="VF531_00405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7712454.1"
                     /translation="MEPLIQEHEVVLFQGDSITDWGRNYQDGNDLGKGYALQTAAWFS
                     ALYPERRVRFINRGISGNRAVDLRGRWDQDCIALKPTTVSIMIGINDCWRRYDNNDPT
                     SPGQFEENYREILSRIRKELGAKLIICEPFVLPFPEERKQWREDLDPKIQVVRKLATE
                     FHAVYVPLDGLFAQAAMRTDPAFWTLDGVHPTEAGAALIARAWLQAVQAL"
     regulatory      9412..9494
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9812..10273)
                     /locus_tag="VF531_00410"
     CDS             complement(9812..10273)
                     /locus_tag="VF531_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX7712455.1"
                     /translation="MKEYQFESIIKASEIGSGGAYVEFPYDVQKEFGVKGRVKVVCFF
                     EGIEYRGSLVKMGTDCHIVGISKEIRSKIGKNIGDKVKVRLYQDESKRTIETHPLLLQ
                     EFSRDKALQANYEKLSYTRKKEINNQLTGAKKEETLKGRLERILSELKEKR"
     gene            complement(10995..11071)
                     /locus_tag="VF531_00415"
     tRNA            complement(10995..11071)
                     /locus_tag="VF531_00415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11035..11037),aa:Arg,seq:tcg)
     gene            complement(11175..12302)
                     /gene="wecB"
                     /locus_tag="VF531_00420"
     CDS             complement(11175..12302)
                     /gene="wecB"
                     /locus_tag="VF531_00420"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7712456.1"
                     /translation="MARLRLMTIIGTRPEIIRLSEVIKKCDLYFDHLLVHTGQNWDYT
                     LNEIFFQELAIRKPDYFLEAAGRNLGETIGNVITRSYNLLQEQAPDALLVLGDTNSAL
                     CAIAAKRLKIPIFHMEAGNRCFDPNVPEEINRTLVDHLSDINLPYTEHGRRYLLSEGI
                     RKEHIYVTGSPLPEVFARHRAEIDASTALARLNLEKRKYILVSAHREENLEIERNFFH
                     LVDTLNQIAEYYGMPLVYSTHPRSYMRISERKIKFNPLISNLKPFGFFDYNKLQQNAF
                     CVISDSGTLSEESALLRFPAALIRTSTERPEVLEKGSLVIGGLIADEVIPAIDMATAL
                     FDREPAPPLPADYNDTNVSTKVVQIIQSYTRIIDRVVWDKP"
     gene            complement(12473..12549)
                     /locus_tag="VF531_00425"
     tRNA            complement(12473..12549)
                     /locus_tag="VF531_00425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12513..12515),aa:Pro,seq:tgg)
     gene            complement(12832..13323)
                     /locus_tag="VF531_00430"
     CDS             complement(12832..13323)
                     /locus_tag="VF531_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX7712457.1"
                     /translation="MIKDTVIRVGVVGDIHGNYEGLERVVERLGRIEMLLFTGDGYRE
                     IDKLTETLRTPVVGVAGNCDLVTEYPAEQTLFLGDTKILLTHGHIYGVKNGLTRLGLA
                     ARAAGAQLVVFGHTHQPLVDNWQEINLLNPGSLSGERSYRGPSYGVIEVSPAGIKLSL
                     ERL"
     gene            complement(13320..13958)
                     /locus_tag="VF531_00435"
     CDS             complement(13320..13958)
                     /locus_tag="VF531_00435"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEX7712458.1"
                     /translation="MRKLVLATRNKGKVRELRELLAGLAVTVEDLDSYPGAPEVAETG
                     TTFTENAILKSKGIAAFSGELTLADDSGLEVDWLRGEPGVYSARYGEAGWTDRQRYEY
                     LLTKLEGVPEPQRTGRFRSVAALVDPARGIVEVTEGEVAGVIAFGPRGDNGFGYDPVF
                     YLPDFGCTMAELSEETKNRISHRGRALRNMLPRISTCALPADASQTRGISGS"
     gene            complement(13927..14697)
                     /gene="rph"
                     /locus_tag="VF531_00440"
     CDS             complement(13927..14697)
                     /gene="rph"
                     /locus_tag="VF531_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7712459.1"
                     /translation="MERIDGRKTDQLRPVKIHRDYIMHAEGSVLIEVGNTKVICTATI
                     EDKVPIWLRGQGVGWISAEYAMIPRATPQRNIREVSKGKLTGRTHEIQRLVGRSLRGV
                     VNLAQLGEKTIWVDCDVIQADGGTRTASITGSFVALADALAKVVPENNPLPLYDFLAA
                     TSVGIFDRTQLLDLCYAEDSKVSVDMNLVMTGSGRIVEIQGTAEGEPFSWDECQGLMG
                     LARQGIMDLIRLQKEVLGPVAGRIGGIGGAKTRPGHEE"
     gene            complement(14749..15306)
                     /locus_tag="VF531_00445"
     CDS             complement(14749..15306)
                     /locus_tag="VF531_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HEX7712460.1"
                     /translation="MTAKRINLPLSQQDIMDLQAGEAVLLSGALFSARDAAHKRLIAA
                     LGAGEPLPVNLSGETIYYVGPCPTPPGKIIGSAGPTTSGRMDPYTPCLLELGLKGMIG
                     KGQRRSEVIAAMVQHRAVYFAALGGAGALIARSIRKAEIAAYPDLGPEAIFRLEVRNF
                     PVIVAIDAYGTDLYQKGRVEYELRQ"
     gene            complement(15510..16355)
                     /locus_tag="VF531_00450"
     CDS             complement(15510..16355)
                     /locus_tag="VF531_00450"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7712461.1"
                     /translation="MREIEAGEIVTLVAQLCREANTRLGQDLMDALAAANRNEPSPVG
                     RECLERIRENASLAETEQVPICQDTGMAVVFVDYGQEVTIRGGELNTAVHEGVRSGYR
                     EGFLRKSVVRDPLIRVNTGDNCPAVIHTRIVPGSRIRITVAPKGFGSENMSRLAMLTP
                     AQGREGVQQFIVETVGLAGANPCPPVVVGVGLGGSMEMAAVLAKRALLRRIGSENFDP
                     DLAKFEAELLTSINNLGIGPQGLGGRYTALAVHLNTYPTHIAGLPVAVNISCHVTRHK
                     TGILG"
     gene            complement(16582..17586)
                     /gene="argF"
                     /locus_tag="VF531_00455"
     CDS             complement(16582..17586)
                     /gene="argF"
                     /locus_tag="VF531_00455"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7712462.1"
                     /translation="MRTIIGNYQVDLWYHSVIRVDDEEEKDMPLRHLLNFKDWSAAEI
                     KGLLDLASQIKGDQEKYWEALDHRTLGMIFQKTSTRTRVSFEVAMTQLGGHAIYLDWR
                     STNLVLAELRDEIRYLSRNVDVLMARVLTFDQVREMADFSAVPVINGCDNKYHPTQAL
                     ADFLTIREHLGRLEGLNLVYVGIHNNVANSLVEGGTKLGMKITVVTPEFNEASRDDEL
                     LTAASQTGLFEQTLDLKAAVAKADVVYTDTWVDMEFFLDPKFEAEKQRRIAKMIPYQL
                     NEAILRDSKALIMHDMPIHTGYEIDRWAIEHPRSIIFDQAENRLHAEKAVLLNLLGKA
                     "
BASE COUNT         4666 a         4657 c         3895 g         4695 t
ORIGIN      
        1 tcttttgatt taaagcgttc gccagtatta aggaaaggaa aattgtaagc ggaacaactc
       61 cgatggtgta gtacatcatg ttgatgaatt catggcccac ctgttggtca atgaacaaat
      121 ccaggtaatt cttgagtccg ataaataccg gctgggtgag actgtcccat ctggtgaatg
      181 acattaaaaa cgaatagata atcggaccga tatggaaccc gacgatgccg atgaatatgg
      241 gggcaataaa aagataaccc caaatccact cgggtttcat ccgccttttt ttctttttat
      301 ggattaagga atcaatatcc gcacttaccg cagcctgact tttcactttg gccaacgctc
      361 cccatcacta tgaatttttc caataacacg gctaattgtg atagtgatat tggtgattga
      421 gtcaagggag aagaaacaaa accatatgcc ctaaagaatc ttctcccttg aatacttcat
      481 tattaccgaa gaattactta ttcatttcgt tattcatcgc tttttgagca tcggctaaac
      541 ccgtagccac atccttcgtt tttaaccaaa tgttctgcaa ctcgttgtcc agtgccgacc
      601 ataccgccgc gctatttttg ctggataccg gcagcggaaa ggcgtatttc gtttcatcga
      661 tgaagacctt tacattactc accgcaggga aattttgaat ccactgggtt tccgctccct
      721 tatatgccgg aatgactgct ttcgcctgat acaatccggc ttctttcgtt gccaagaatt
      781 tcaagaactt ccatgcctgg ttgggatatt tggtcttggc ggatgcggca aaacccaaac
      841 catggatgat cgtcgcccgt tgtttccggt atggcaaagc ggcgactcca agcttatctc
      901 ccaagacctt ggaataatca ttgatcatcc aggaaccgtc attgtacatc gccacttgac
      961 ccgaggaaaa agccttcaag taatcgatat ccttttgctg ggcaccgttg ggtgaaacct
     1021 tgtacttata cattaaatca actaaaaatt gaacggtttc cacattttcc ggcgaattaa
     1081 tcaccgcttt ggtcaggtcc ttattatata tctggccggc attttgatag atggcatcgg
     1141 ccacgccccc ctgaatgctg ggcagacaat aatagcccca ttgtttaacc gaaccatcct
     1201 catttttcaa agtcaacttt tgggctgcag ctagaagatc gtcccaagtc caatcatcgg
     1261 tcggataagg gaccttcgcc tcatcgaaac attgtttatt gtataaaagc gcaatcgtat
     1321 cataatcttt gggaatcgcg tataactttc cattcaagct gtaaatcttc ttcaagctgt
     1381 ccgggaaatt gctcaaatcg gttttttctt tatcgatata tttctggaga ttgaggatca
     1441 atccgaccga catatacttt ggagcgttaa tgccgtttag ccataaaatg tcggggccgg
     1501 acgaagtagg gagagaggtc tgaattttca tccagtattg atcccagggt attaccgtgc
     1561 ttttgatggc aataccggga tttctctttt caaaatcctt aatgatttgg tccatcgccg
     1621 gtttttggtt ttcatcccaa taatagaagt taagcaccac atttttactg ctgccgtagc
     1681 ccgtaaaggt cagcatgaac atcaatgcca caaaagcact caatacgatc actattcttt
     1741 tcatttgaat cctccagtta aattttttta ccatgggtag gggagcaaac agatattccg
     1801 ttcctccgta ttccatccaa aatccgtgcc taatactaat ccaggttgat aatccgttcc
     1861 acatcttgct tgcatgcttt gattgcggct tcaacggtct tcttgttatt gtaggcggac
     1921 tcaaggtcct gattgattcg ctcaaagatc tcgccctttt tcgggaatga aatataagga
     1981 acgataccta cggccgattc gaaataagcc ttcttattca atggcttttg cgacgggttc
     2041 aggaaggccg gagacatggc aacctcctta agtaccggaa tggcgtctcc ttccttggcc
     2101 ataatggtct gcccctcttt gccgcataaa aaccggatca acttccaggc ttctttggga
     2161 tgctttgttt tggcatacat cgagttcccg acgctctgga cgcagtatat ctttttgccg
     2221 aaagtcggtg ccacagccac atcccaatca aaagttaccc cgtttaattg ggaaacctgc
     2281 caggatcctt gataaaccat ggcaaatttc ccggtctgaa agtagacatc ctgaaatttg
     2341 gcgactgccg ccggagtggg tttgacatga tatttccagg ttaggtcgta taaaaactgg
     2401 atcacttttt ttactttcgg agagtcaata aagaacttct gcccgtcggg gctgattaac
     2461 tgaccgccgc tctggaaagt gaatgtttcc aatacttgat caatatcggc ggttgcaaca
     2521 aataagccgt attggtcaat attgtcggga tcaaagcccg gatcattggc gtttctcccc
     2581 ttgttgtcca gtgtaagctt cttcgcgagt tcaatgactt gatcccagga catgttgccg
     2641 ctcggataat ctttgatgcc cgccttggca aataaatcct tgttataaaa catcacgatg
     2701 gcattataat cgcgcgggat cgagtatatc ttgttcttat aagtaaacgt atcccagaca
     2761 ttgtaataat cggtcttttt aataccatcc cgtttcatga gcggagccaa atccattaaa
     2821 actcccttgg gggcgaattg attaagatac cagccgccat cgaataaaaa cacatccgcg
     2881 acctcatcac tggcaacctg cgtttgcagc ttttcccaat atgtatccca acttgacgct
     2941 tccagggtta ctttgatgtt gggattggct ttcataaagg cattcagcat gactttggtg
     3001 cctgacagtt catcgggtat gccccatcgc gcgtatcgta aagtcacctc cttacccgcg
     3061 gcgccgataa atccgcccat cgttaccagc atgaccagtg ccattaacca gaaataagtc
     3121 tttttcatat ctctccccct tttcttattt ttgaaggttt taccctcccg ccgagcttga
     3181 atcaaccttt gattccggtt aatgtaatcc cctcaacgaa gtatttctgg gctgagaaaa
     3241 agagaatgat caggggcagc acactaatta cggtagccgc ggacataaaa ccgacctctg
     3301 ccacccgttg gccttgcaat aatgtaattc ctaccgccaa tgtcatttta tccatcttgg
     3361 tcaggaagat cagcggcccg taaaagtcgg tccatgtcca gataaatgag aaaatgccga
     3421 cagtaatcaa aatcggtttg cacagcggca aaataatccg ggttaatatc tgcaaattgg
     3481 aggcgccgtc gatcaacgcc gattcatcca attcgtaagg aattgtcatc ataaactgcc
     3541 gcatcaaaaa aatgaagaaa gcattcccaa aaaaggccgg aacaatcagg gcgtaaaaag
     3601 tgttcagcca atgcagatat ttgtaaagta tgaagacggg tatcagaatg acctgaccgg
     3661 gaatcatcat tgtgcccagt aaaacgataa aatagaaatt tttcaatttg catttcaatc
     3721 tggcaaaggc gtaactgact aaaacagtgg aaaagatatt cccgattaat accccgatgg
     3781 ttaccttaaa ggaattgatg atgaaagtat ccatcggcag ataatgatga tcgagcaatc
     3841 tgagataatg tttgaaaaca accggttttg ggatccatac cggcggatat gtccaaatct
     3901 gggcgttctc tttaaacgaa ttcgagacaa gccatatcaa aggtgccata aacaagatgc
     3961 cgccggctat caagaataag cattgaatga ctttgatgag tgtagttttt gagataaatc
     4021 ccggcctgtt tttggcaacc gtattcatgg ctcacttcct ctccccttca taaaacaccc
     4081 aatgttttga agaacgtaga ataatgactg ttaaaaccaa aatcaccagg aataatatcc
     4141 acgccatggc cgacgccata cccattctcc cgaacttgaa agcggtctga taaaggtagt
     4201 acatgtagaa atacgtagag tattcgggac cgcccgcagt cattacgtag gcggaagtaa
     4261 aggtctggaa agtattgata actcccatga ttaaattaaa gaagattgtc ggggtaatca
     4321 tcggcagggt aattgcccaa aacttatgcc aggcattggc gccgtcaatc gcggccgact
     4381 catataaact gccgggaata tccttaagat tggcgagata aatcaacatt ccatcgccga
     4441 tagtccatag acccatcaaa atcaaggacg gtttggacaa taccgggtta ttcagccatt
     4501 cctggggagg aatatgacaa aaccgtaaaa aaacattaat taagccgaat ctatcatcca
     4561 gcatgatacg ccataaaatc gcacttgaaa ctgccgggac cagaaccggt aaataataaa
     4621 tggtccggaa aaacgcaatt ccttttaaac gcaagttcat aaatagactt aagctaattt
     4681 gggcaattat ccctatgggt aaggcgataa ataccatgta taatgtgttc gccagggact
     4741 tgtaaaataa atcatccatg gtgaacaaaa agcgataatt ctttaacccg ataaattccg
     4801 ggcttgatat aatatcatat ttggtcaagc taaggtacaa agatatcaat gccggtccga
     4861 taaaaaacac caatacgccg atgagccagg gagaggcaaa caaataaaat ttgagcgatt
     4921 ctttatgcct caatttatag aatatttgtt ttgaaaaatt tgccttggcg tcttttccca
     4981 tccgttatct ccttgaatga gacttgtttt aatggcatca ccgttcattt ttaatgttta
     5041 taaaactgcg gcagccactc ctgattgact gcaaacatct catcaaccat ttttttaatc
     5101 tcatccaggc taagtaccgc cgcggtaagc ggatcaaaat aaatcgattg gtaaatcaga
     5161 tcccgttcgc cggaaatact gccttgtaca gccaattctt cacagcgggc cgctgtattg
     5221 gtgatgattg ccacattatc cggtaacggc ccgacatgca tcggttcaaa cccccgccgc
     5281 gacgccaaaa ccggtacctc aacgcaacaa ccctcgggaa gattatcgat taagccgaaa
     5341 ttccgcacat ttccattgaa tttgtagagc tccccatccc cgatggtcgc atttaagata
     5401 taagccgcat attcttcccc tctttgcaga tcaagcggtt ccttactgtc cagccattct
     5461 tgagtcttat tcttccaggt ggtatccgca tcaaggtatt cttgcaagat ataagcgtag
     5521 tgcccggggt tccaaccggt accatgagta cagaacttct cgattaaatc cggtcgcttg
     5581 cggaaccagg ggttatattc cgaattatgc ccgctggact cggtggaata ataaccaaag
     5641 tgccggaaca tctcattacg caccatttcc tgatgataaa tttccggttt ttccaaggct
     5701 ttcttgatta atggataggc gtcctgccct cgccatcgat aatctaaata ccaggccata
     5761 tgattgatac cggcgcatag ataggtaatc tcattcatcg gagcgtagat ccactccgcc
     5821 agcatctcgg ctgttccctg gacgctatga cataagccgg tcaccttgat tttgctttgt
     5881 ccttgcatcg cccggcataa catggccatc ggattggtat agttcaagaa aacggcatcc
     5941 gggcaatatc tctcaatatc ctttacaata tcaagcatta ccggaatggt gcgcagcgcc
     6001 ctaaatactc cggccggacc ccgggtatca ccgacacaga tgtcgactcc gtactttttg
     6061 ggaatctcga tatcatgccg ccacacattc actccgccgg ccaaaatggt acataatacc
     6121 ccgtcggcgc cttttaaagc ttccgctctg tctttggtcg ccactacttt ggcaggatag
     6181 ttcccggcgg caataatctt ttccactgcg gccttggcaa aagccaatcg ttcatcgtta
     6241 atatccatta aggcaatggt gctatcggcc aaagccggaa aagtcaagat atccctgact
     6301 aattcccggg tgaacccaaa acttccggct ccgataaacg tgatcttttt agccataaaa
     6361 atccctccta aaaaccatcc ctgcgcatcg agtaaaaaag gattcgtcaa tgcttataac
     6421 ttaatccagg catccaaacc ctcgggctga tcgggatcga ctccattctg aatcgccttg
     6481 gaatatgaca gcatttgtaa gagaagaatg aacggtatgc cgcgagcagc aaaatccaag
     6541 ttagccccca acgcaacctg aaggcttacc cctggaataa gcggcatcgg tgtatcgcta
     6601 taaacgatta ccgtggcccc tttttgcaac agatcattga ttaaatcttt ttggaacctg
     6661 aggtctccgg ggttcaaagc cattacaacc agggttttgt cacgaatcaa aaccatcggg
     6721 ccatgccgta catccagcaa atgataatac tgccccggta ccctcgcaat ttcggtgaag
     6781 gccaaagctc cttcccgcgc aatgccttga atctccccgt ccgctaaaac cacggcctca
     6841 cgccaattct ctttggcaat ttgagccaga tccgattcgt atctttccat ataggccgtg
     6901 ccgccatcaa tcgccgcatc aatcgaggcg atgagtcgct catcaccgct taagatcgca
     6961 atgattaaca gattggccgt ataaagattg gttacggtcc gggtctggca gacgctctca
     7021 tcaaaagccc agggaagttc cagagtaaaa tcggccaatt tggacagggc ggagccggca
     7081 acacaggtta tggccaagac cggcacgttt tgaacggccc ggacattttg gatggccatt
     7141 agcacttcgc tggtgcttcc ggaacgcgtc ggagtgatga ctagcgtcca ctcaagaact
     7201 tttaaatagt gttcataatt gagcatcaaa tctccagccg ccatggccgt tgccggcagg
     7261 ctcagtctta atctggcgga gaactccgcc gattggcaaa gatagaaact agacccgcat
     7321 ccgatgtagg ttaagcttcg cggagcctga tccttataaa aacgcagcag gttctccttt
     7381 tgggaaagaa tataattaaa agtcttccgt aacgctgaat attgagattt tatctcggta
     7441 taagtatggt ccacagtgtt gactcctttt taatgttagt ctttatattg ttatcttatt
     7501 atattgttat gaccatttat tctacaataa atgccccctt acgatgcgat tgctgcaatg
     7561 ctcatgatag aataactcct gtttcctatt ttaaaacaac ctgatactta tagcgatcgc
     7621 cgcgaatgat gccatgacaa aactcgacga ttcgttcccc ggcgtaagtg gttcgctcca
     7681 gtaaaatacc gggtgaattc ttgcccaccc gcaaatgtgc ggcgttattg ggattgatga
     7741 ttaccgcttc aaaggtttca atggcctggt ccggaaccaa attatacttt cttttcatgg
     7801 tgttgtataa accgtttgtg ccgatctcgg cggcagtcat cccggggcaa aggtcacagg
     7861 gaatatagga tgtctccagg gcaaagggtt catggttggc aagccggaga cgtttgatgc
     7921 tgtatacggt catcgcgttt tcgttgaaat gtttagcgac aacctcatcc agggttaaaa
     7981 ttttgaaatc aatcatttcc gtgctcggag tgaaacccat attgcggatc tcttcactga
     8041 aactatagaa gttcgataaa cgttgttcaa ttttgggtgc ggtcacaaag gtgcctttcc
     8101 cttgtttccg gtataaaaag ccttcatctt caagctcttc caatgcttga cggacagtta
     8161 tccggctaac tttatataaa tcgcataact ccctctcagt gggaatcttt gtgtcaatac
     8221 cccattcgtt actcttaatc ttcccgacca gaatctcctt taactgatga tacagagtga
     8281 ccggggcctt ttcatccagc aaagcattca cttcttttca attggttata acaatataat
     8341 aagttatcca agaaaaaatt gcaacccctt gaaacgaaat ttttgaccaa agaaatgaca
     8401 aatcaaaaga ccgtctgttt ctgaacggtc ttctgatttt ttattatgaa agaacaactc
     8461 caacttgctt tccctttaaa gggcttgcac cgcctggagc catgccctgg cgattagagc
     8521 ggcaccggct tctgtaggat gcactccgtc caaagtccaa aaggccgggt ccgttctcat
     8581 cgcggcctgg gcaaacagtc cgtccaaagg cacgtaaacg gcatgaaact ccgtggccag
     8641 cttccgaacc acctggatct tggggtccag atcctcccgc cactgtttgc gttcttccgg
     8701 gaacggcaat acaaacggct cgcaaatgat caacttcgcg cccagttctt ttcggatccg
     8761 ggagagaatc tcccggtagt tctcctcgaa ttggccggga gaagtgggat cgttattatc
     8821 ataccgccgc cagcaatcat taatcccgat cataatcgat acagtggtcg gtttcagcgc
     8881 gatacagtcc tggtcccacc tgccccgcag atcgacggcc cggttgccgc tgatgccgcg
     8941 gttgatgaaa cgcacccgcc tctcgggata caacgcggaa aaccaagctg ctgtctgtaa
     9001 ggcatagccc ttgcccaaat cgtttccatc ctgataattc cggccccaat cggtgatgct
     9061 gtcgccttga aacaatacca cttcatgttc ttgaatcaat ggttccatgg atctcttcct
     9121 tccctatgtc tgattcgttt ttataccgat aaatccggtt cctcaagtga ataaactgag
     9181 gaatagcaga cctattctat atacgctata ttttgaattt tacctttaga ataatataaa
     9241 agggacatgc actccatacc atgattgtta tcgtgagaat ggaggatcaa aagttacatc
     9301 aagcaatata gagctcaagg ggcgagtgtg tttccgggag gaaccttaaa acctcgcatg
     9361 gtcattgcat taacattatt catatgttaa tatatttttt aaagcaacat aaacagataa
     9421 tagcaaatct agcgaaagct agagacgcaa agccatgggt ctaaagcgaa agccatgatt
     9481 gccaggttac ctaaattagg ttgttttttt gtttgccttt tttaagaaaa aaatggtggt
     9541 ccagcgcatt taattaaata tatggtgcaa acccaataaa ccgtcactaa cttaacgaag
     9601 gtgataaaat gtttgaccgc cttataaaat gtgccggttg taaaaactgg cgaaactgcg
     9661 aaaaacattg tgtatttgaa tttagccggt tcgaattgat caagggggag atcgataaga
     9721 agttgcttaa catttgccaa atggttaaca gcttttgcaa tttaatcaga agaatttttc
     9781 gtggattaat gtttcgatac tagcatacaa atcaccgttt ttcctttagt tccgaaagaa
     9841 tcctttccag cctgcctttt aacgtttctt cctttttcgc gccggttaat tgattattga
     9901 tctccttttt tcgtgtatag gaaagcttct catagtttgc ctgcaaagct ttatcccggc
     9961 tgaattcctg aagtaacaac gggtgggttt cgatcgttct tttgctttca tcctgataaa
    10021 gacgtacttt taccttatct ccgatgtttt ttccaatctt gcttcggatt tctttcgaaa
    10081 tgccgacgat atgacagtcc gtgcccattt tgaccaagga accccggtac tcgatgcctt
    10141 caaaaaagca aaccacttta acccgtccct tgaccccgaa ctctttttgg acatcatagg
    10201 gaaattcgac ataagctccg ccactgccga tttccgaggc tttaatgatg gattcaaatt
    10261 gatattcctt caaggccgga cctccgccaa attatcgtga aacgtatgtg ttttaaaatc
    10321 cacaaatgtc cccaaaatca aagcgcttgg atcctttacg tgcggaaata atgacatcct
    10381 cgttcatgga atcacccaaa agctagggta ccaaaagcag attgatttat accaagtcgg
    10441 cttcgaaggg agccctccct cattctgtct ttctccccga gggagaaaca aaatgcccat
    10501 tctgagagtt cttccggagg tagaaacgaa atgctcgttc tgttagtttt catgaggaga
    10561 aacaaactat tcattctgtt tgtccaagca tatagtacga tcagctacaa actcagcacc
    10621 gattcagccc tcgaacttga ctcccgactt ggccttcgtt cccccctctg gggggaggca
    10681 aggtggagga ctccaccgaa gactctttca taactctcct tgaacttcat atcaagtcat
    10741 cttctattaa atatttcatt aatacgggag tacttcctgc tatttaccaa acaaaaaagc
    10801 accatccatc tctgaacggt caaatcctca tcaatggtgc gcccggatgg tgaagactct
    10861 attggagtct tacgaacccc cgacctacag attcatcgtg tgcatcagca cacgcgtctg
    10921 ccactttatc ctaaagcata tcaggtctat aaaaaaagat tgtccgttgt tcggacaacc
    10981 taatctttat cttttggtgc gccaggaggg gctcgaaccc ccgacctaca gattcgaagt
    11041 ctgccactct atccagctga gctactggcg caggtttatt tctttagcat agattttatc
    11101 tatcgggcgc gtacctaata ttataacaca cttacccgac cactacaatc gcgactcatt
    11161 accagatttt taactcacgg cttatcccag accacccggt cgatgatccg ggtgtaactc
    11221 tggatgatct ggaccacctt ggtggagacg ttggtatcgt tgtaatccgc aggcaaaggc
    11281 ggtgccggtt cgcggtcaaa gagcgccgtg gccatatcga tggccggaat cacctcatcc
    11341 gcgatgagcc cgccgatgac cagcgatcct ttctccaata cctcaggccg ttcggtgctg
    11401 gtccggatca atgccgccgg aaaacggagc aaagccgatt cctcactcag ggtgccgctg
    11461 tcggagatga cgcaaaacgc attttgctgg agtttattat aatcgaaaaa cccgaatggt
    11521 ttcaaattac ttattaacgg gttaaactta atcttccgct cggagatccg catatagctg
    11581 cggggatggg tcgaatagac caagggcatc ccatagtatt cggcgatctg atttagggta
    11641 tccaccagat ggaaaaagtt tcgttctatc tccaggttct cctcacgatg ggcgctgacc
    11701 aaaatatact tccgtttctc caggttcaat cgcgccaacg ccgtgctcgc gtcaatctcc
    11761 gcccggtgcc tggcaaaaac ctcgggaagc ggcgatccgg tcacgtaaat gtgttctttc
    11821 cggatcccct cgctcaacaa gtacctccgg ccatgctcgg tatagggaag attgatatcg
    11881 ctcaaatgat ccactaacgt ccggttgatc tcctccggca cattcgggtc gaagcaacgg
    11941 tttccggctt ccatgtggaa aatcgggatt tttaagcgtt tcgcggcgat agcgcacagg
    12001 gcgctgttcg tatcacccaa aaccagcaaa gcgtccggcg cctgttcctg caataggttg
    12061 tatgagcggg taatcacatt cccaatggtt tctcctaaat tcctacctgc cgcctccagg
    12121 aaataatcgg gctttctgat ggcgagctct tgaaagaaga tctcgtttaa ggtataatcc
    12181 cagttttgac cggtatgcac cagcaggtga tcgaaataaa ggtcacactt tttgatcacc
    12241 tccgagagtc ggatgatctc gggccgggtg ccgatgatag tcattagtct caatcgggcc
    12301 aaggtttaca cctcatgata ataacatttg ggaaccgagg cttgtccatg aagcggtatc
    12361 ggcctcgagg atccaatata agaatatggc ccggtgttgt ggaatattac aaaggaatac
    12421 acaaaaaaga aaaccggcgg attgaataat ccgtcggtcc tgtatttcaa aatggtcggg
    12481 gcggcgagat ttgaactcac ggccccctgc gcccaaggca ggtgcgctac caaactgcgc
    12541 tacgccccga tatatatcaa aatgccttat tagtatactg tattaaccga ccgctgtcaa
    12601 gagtctccaa gtcaagtcga aagtcgattg aggcaatagg caaaagtgtt ggaagtgatt
    12661 tattatggtc aaactccaaa aacaggcaga taacactcgt gtctatctct cccaccatca
    12721 ctctcggtat tgcaagcatt aaacccgtaa aggttggcaa atttccttta agagataggg
    12781 tagtaggccc acaccctatt tcctgttgcc tattgcctgt tgcctaagta actacagcct
    12841 ttccaaacta agcttgatcc cggccggaga gacctcgatc accccgtagg aaggccccct
    12901 gtaggaacgc tcacccgata gggaaccggg attcaagaga ttaatctcct gccagttgtc
    12961 gaccagcggc tgatgggtat ggccgaatac caccagttga gctccggcag cccgtgccgc
    13021 cagacccaac cgggtcaatc cgttttttac accatagatg tgaccgtgag tcagcagtat
    13081 tttggtatcc ccgagaaaca gggtttgttc cgccggatat tcggtcacca ggtcgcaatt
    13141 gccggctacc ccaaccaccg gggtgcgtaa cgtttccgta agcttatcaa tctcccgata
    13201 tccgtccccc gtgaaaagga gcatctcgat ccggcccaaa cgctccacta cccgctcaag
    13261 gccttcgtaa ttgccgtgaa tatcgccgac cactccgacg cgaatcaccg tatctttgat
    13321 catgatccgg aaattcctcg cgtttgtgac gcatcggcag gtaacgcgca agtgctgatt
    13381 cggggcagca tattccggag ggcccggccg cgatgactga tccggttctt ggtctcctcg
    13441 gaaagctctg ccatggtaca accgaaatcg gggagataaa aaacgggatc gtaaccgaaa
    13501 ccgttatctc ctctcggccc gaaagcaata acccctgcca cttccccctc ggtgacttcg
    13561 acgatccccc ttgccggatc gactaaagcc gctaccgaac ggaaacggcc agtccgttgc
    13621 ggttcgggaa caccttccaa tttcgtgagc agatactcgt accgctgacg atccgtccag
    13681 ccagcttcgc cgtaccgggc cgaataaacc ccgggttcgc cccggagcca atcgacctcc
    13741 agaccggaat catccgccag tgtcagctca ccgctaaaag cagcgatacc tttggatttt
    13801 aagattgcat tctcggtaaa tgtggttccg gtctcagcca cctcaggcgc accggggtag
    13861 ctgtccagat cttctaccgt caccgctaat cccgccaaaa gctcccggag ttcgcggacc
    13921 ttccccttat tcctcgtggc caggacgagt tttcgcaccg ccgattcccc cgattctacc
    13981 cgccaccggc cccaagactt ctttctgaag ccggattaag tccataatcc cctgcctggc
    14041 caaacccatc aaaccctgac attcatccca ggaaaacggt tccccttcgg cggtgccttg
    14101 aatctccaca attcgtccag agcccgtcat caccaggttc atatccactg aaacctttga
    14161 atcctcggca tagcaaagat ccaatagttg agttctgtca aatatgccca cgctggtagc
    14221 ggccaaaaaa tcgtacaacg gcagcgggtt attctccgga acgaccttgg ccagcgcatc
    14281 agccagcgcc acaaaactcc cggtaatcga tgcggtacgg gtacctccat ccgcctggat
    14341 gacatcacaa tcaacccaaa tggtcttttc ccctaattgc gccaggttga caactcctcg
    14401 gagggaccgg cccaccaaac gctgaatctc atgggtccgg ccagttaatt ttcccttgga
    14461 gacctcccgg atattccgct gcggcgtggc ccggggaatc atggcgtatt cggcgctgat
    14521 ccagccgacg ccttgtcccc gcaaccagat tgggacttta tcctcaatgg ttgccgtgca
    14581 gatcaccttg gtatttccaa cctcgatcag gaccgagccc tcggcatgca tgatataatc
    14641 gcggtgaatc ttcaccgggc gtagctggtc cgttttccgt ccgtcaatgc gttccatccg
    14701 tttggcctcc gattttggtg acttccgtgc gcgcataggc gcgcgatttc attgcctcag
    14761 ttcatattct acccgtccct tttgatagag atcggttcca tatgcgtcga tcgccacaat
    14821 cacgggaaaa ttccgcactt ccagacgaaa aatggcctcc ggtcccagat cggggtaagc
    14881 tgcaatctcg gccttccgga tcgagcgggc aattaatgcc ccggctccac ccaacgccgc
    14941 gaagtatacc gcccgatgtt gcaccatcgc cgcaatgact tccgaccgcc gttgtccttt
    15001 accgatcatt cccttgagcc cgagttcgag tagacaggga gtatagggat ccatacggcc
    15061 gctggtagtc ggtccggcgg aaccgatgat ttttcccgga ggagtcgggc agggaccgac
    15121 ataataaatg gtctcaccgg acaggttcac aggtaacggt tccccggcac ctaacgcagc
    15181 gatcaacctt ttatgtgccg catccctggc tgaaaataaa gctccgctca gtaatacagc
    15241 ctctccagcc tggagatcca tgatatcctg ttgggataag ggcaggttaa tccgtttcgc
    15301 agtcacataa atctcctcta atggttgaac taaggaatcc tttgttgctt gtggttaaat
    15361 tcttcaatgg ccgcaaataa agacatactt attttcgtgt cttttgtgtg tttcgttgtg
    15421 aatagaataa gcatacagat agaacctttt aaccacaaaa atcaccaaat atagacccaa
    15481 aagcgaatca tgcccccgca aatgtttttt taccccagga tcccggtctt gtgccgggta
    15541 acatggcatg atatattcac cgccaccggc aaaccggcga tatgggtcgg atatgtattc
    15601 aaatgaaccg ccagtgctgt ataacgtccg cctaaaccct gcggtccgat acccaggtta
    15661 ttgatggatg tcaggagttc cgcttcgaat ttcgctaaat caggatcgaa attctccgag
    15721 ccgatcctcc gaagcagggc ccgtttggcc aaaaccgccg ccatctccat gcttcctccc
    15781 agccctaccc cgaccacaac cgggggacaa ggattggccc cggccaaacc gacagtttcc
    15841 acgatgaact gttggacacc ttcccggcct tgagccggtg taagcatggc gagacggctc
    15901 atgttttcgc tgccaaaacc ctttggcgcc acggtaatcc ggattcggga acccggaacg
    15961 atccgggtat ggatcaccgc cggacagtta tcgccggtat tcacccgtat caaaggatcc
    16021 cggactaccg atttacggag aaacccttcc cgataaccgc tgcgtactcc ctcgtgaacc
    16081 gcggtattca attccccgcc ccggatcgta acctcctggc cgtaatcgac gaaaaccaca
    16141 gccattccag tatcctgaca gataggaacc tgctcggttt cggcgaggga agcattctcc
    16201 cgaatccgtt caagacattc ccggccaacc ggcgagggtt cattccggtt ggcggctgcc
    16261 aaagcatcca ttagatcctg gccaagccgg gtattggcct cgcggcacaa ttgcgctacg
    16321 agtgtcacga tctctcctgc ttcaatctcc cgcacctaca gctccctcca cgctccgcta
    16381 caattggcta tcccctgtta acccttccgg cagatccgtc acacgaacct acttgagaca
    16441 ggccggccta ccgccgtggg tccgatgata acggaggcac tcgcaacaga tcccgtgtcg
    16501 ggaacagctc tgcttcttac aggtacagtc ctttttattt tgatcaatat tttggcactc
    16561 cataccggac acctcgctct actatgcctt gcctaacaag ttcaggagaa cagctttctc
    16621 ggcgtgcaag cggttttcgg cttgatcgaa gatgattgaa cgagggtgtt cgattgccca
    16681 gcggtcgatc tcgtagccgg tatgaatcgg catatcatgc atgatcagcg cctttgagtc
    16741 gcggagtatg gcctcgttca actggtaagg aatcatcttg gcaatccggc gctgtttctc
    16801 cgcttcaaac tttggatcga ggaaaaactc catatcgacc caggtatcgg tatagaccac
    16861 atccgccttg gctactgcag cctttagatc caaggtctgc tcaaagagac ctgtttggga
    16921 tgcagctgtc aataactcat catcccggct tgcttcatta aactcaggtg ttacaaccgt
    16981 aattttcatt cccagtttgg tgccgccctc caccagggag ttggctacgt tattatggat
    17041 tcccacataa accaggttta gtccttcaag cctgcccaga tgttcgcgaa tggtcaggaa
    17101 atcggccaag gcctgagtgg gatggtattt attatcacaa ccgttgatca ccggcactgc
    17161 tgaaaagtcg gccatctccc ggacttgatc gaaggttagc acccgggcca ttaagacatc
    17221 cacattccgg gagaggtaac ggatctcatc ccgcaactct gccaggacca ggttggtgct
    17281 gcgccagtcg agatagatgg cgtggcctcc caactgggtc atggccacct caaacgacac
    17341 ccgggtccgg gtcgaagttt tctggaaaat cattcccaaa gtccggtgat ccaaagcttc
    17401 ccaatatttt tcttgatctc ccttgatttg ggaggcaaga tccaaaagcc ccttgatttc
    17461 agcggcggac cagtccttaa agtttaacag gtgcctaagc ggcatatcct tctcctcctc
    17521 gtcatccact cgaatcaccg aatgatacca cagatcgact tggtaatttc caattatggt
    17581 tctcattata aacgatcctg cggtgagagt ctacaggttt acccaaaaac aaacgccgtt
    17641 caacctaaac ttcagcggat gaacggcgca ccggataacc taatataaaa atgtaaagag
    17701 taaagggtaa aaaaaagaaa atcgtttttt cacttttccc ttttctattt tctcttttct
    17761 tttatcccca gggttgttgt tttagtcgag tcaccgtagc cccgtccaac cggtacagta
    17821 aggccttgac caaagccaca gtggcgctta gatcctttaa gctgatgatc ccttgatgac
    17881 tatgagcata acggaccggt acgccgatga ccg
//
LOCUS       DASVMB010000014         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_665234,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000014 DASVMB010000000
VERSION     DASVMB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665234"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VF531_00460"
     CDS             complement(<1..918)
                     /locus_tag="VF531_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX7712463.1"
                     /translation="MKTPSHRISIPTILQVNRNNLARLGSFIAQAGFTKITLFFGEGI
                     EALFGEAVTAAIDEGKIQVLQRLNYDDNRIEELLRMAFAIPRNTQAVVGVGGGKVLDV
                     AKYIAYLNELPFISVPTATSNDGFASSGCSLLVEGHRRSVPARIPYGIVVDLDVIGSA
                     PSKLIYSGIGDLVSKITAIEDWEFEAANGKAVLDDFAVMVAKKSVNSVVRMDIQTNIR
                     DIFFLKELVDSLTMSGIAMEIAGNSAPASGSEHLISHALDSFMVKPELHGIQVGVATY
                     LMSIVQNHRSQRVRKFLTETGFFDHVRTLQ"
     gene            complement(915..1787)
                     /locus_tag="VF531_00465"
     CDS             complement(915..1787)
                     /locus_tag="VF531_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7712464.1"
                     /translation="MYDMVIVGGGPAGLAAAVNARRRNKQVALVAKEENSSKLVQAHR
                     IDNFLGIPGITGRELAEKLRHHAEEQGTTFIKDEVQSINPDEGRFMLWGRENSFEALS
                     IVLATGIVLGSEIAGETEFVGRGVSYCATCDAMFYKGKTVAMIGYIPEAETEAEFLAE
                     LCAQVYYLPQYKLQKQLDSRITIISGKPAAIRGDVTVKELATQAEAWEVDGVFIERSG
                     RPAEQLLPGLELSGGFIVTGQDQATNIPGVFAAGDCTGRPWQIDRAVGQGQVAALGAV
                     QYLEMAKSGSGVVG"
     gene            1894..2064
                     /locus_tag="VF531_00470"
     CDS             1894..2064
                     /locus_tag="VF531_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712465.1"
                     /translation="MITLTLTDQEVNLLEMSIRHCLKTCKQGGPDSGCSDCELLQQVA
                     VKIEKTKTTAPA"
BASE COUNT          501 a          620 c          532 g          500 t
ORIGIN      
        1 ttgcagggtc cggacgtggt caaaaaagcc ggtctcggtc agaaatttcc gtacccgttg
       61 cgaacggtga ttctgcacta tcgacataag ataggtggca acacccactt gaatcccgtg
      121 gagttccggt ttgaccatga agctgtccaa agcatgggaa atcaagtgct cgctgccgct
      181 ggccggagcg ctgttcccgg cgatctccat ggcgatcccg ctcatggtga gtgaatcgac
      241 caattccttc aggaaaaaga tatccctgat attcgtttgg atatccatcc gtactacact
      301 gttcaccgac tttttggcga ccatcaccgc aaaatcgtcc agtacggcct taccattggc
      361 cgcctcaaac tcccaatcct cgatggcggt gatcttggaa accaggtcgc cgatgcccga
      421 gtagatcaac ttggaagggg cgctgccgat cacatcaagg tcgactacga ttccgtaagg
      481 gatccgggcc ggtaccgaac ggcggtgccc ctcaaccaaa agtgagcagc cggaactggc
      541 gaaaccgtcg ttggaggtgg ctgtgggcac gctgatgaac ggaagttcgt ttaaataggc
      601 aatatatttg gcgacatcaa gcaccttgcc gccgcccact cctaccacgg cttgggtatt
      661 gcggggaatg gcaaaagcca tccggagcag ttcctcaatg cggttatcat cataatttaa
      721 acgctgcaga acctggatct tcccctcatc tatggcagcc gtaaccgcct caccgaacag
      781 ggcttcaatc ccttcaccga aaaacaaggt gatcttagtg aatcccgctt gagcaataaa
      841 cgatccgagc cgggccagat tattccggtt gacctgaagg atggtaggga tggagatccg
      901 gtgggatgga gttttcatcc aaccactccg cttccggatt tcgccatttc aaggtattgt
      961 accgcaccta aggccgccac ttggccttga ccgaccgcgc gatcgatctg ccagggcctc
     1021 ccggtgcaat ctcctgcggc aaatacgccc ggaatattgg tggcttggtc ttggccggtg
     1081 acgataaagc ctcccgacag ttccaggccg gggagaagct gctctgccgg acgtcccgac
     1141 ctttcgataa ataccccgtc cacttcccag gcctcggctt gggtcgctaa ctccttcact
     1201 gtgacatccc cccggatggc agccggttta ccgctgatga tggtgatccg ggagtccagt
     1261 tgtttttgaa gtttatattg gggcagataa taaacttggg cacagagctc cgccagaaac
     1321 tccgcctctg tttccgcttc gggaatatag ccgatcatcg ccactgtctt gcccttataa
     1381 aacatggcgt cgcaagtggc acagtaactg accccccgcc caacaaactc ggtctctccg
     1441 gcgatctcgg aacccaggac gataccggtg gccagaacaa tgctcaaggc ctcaaacgag
     1501 ttctcccggc cccacagcat aaaccggcct tcatccggat taatgctctg tacttcatct
     1561 ttgatgaagg tggtcccttg ttcttcggcg tggtgtctca atttctcggc caattcccgg
     1621 cccgtgatcc cggggatccc caggaaatta tcgatgcgat gagcttgtac cagctttgaa
     1681 ctgttttctt ccttggcgac caacgccacc tgtttgttgc gccggcgggc gttaaccgct
     1741 gccgccagcc ccgccggccc gcctccgaca attaccatat catacatgcc gatcaccctg
     1801 cctttatggc ttgaaatcca gcgtatttgg aatatattcc tgtttgccat ggatactatg
     1861 atcatacaga attcgaatga aacgaggtca ctcatgatta ctttaacctt aaccgatcaa
     1921 gaagtcaatc tcctggaaat gtcgatccgg cattgcctga aaacctgcaa acaaggtggc
     1981 cccgatagcg gctgctccga ctgtgaactg ctccaacaag tcgctgtcaa aattgaaaag
     2041 accaaaacaa ccgctccggc ttgagcggtt gtttgctcgt aaacctattt cggacataat
     2101 cagttaaatg acattcaaaa ccgcagtatt ggcggagagt aaaaaccttt cgg
//
LOCUS       DASVMB010000015         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_149707,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000015 DASVMB010000000
VERSION     DASVMB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149707"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF531_00475"
     CDS             complement(<1..184)
                     /locus_tag="VF531_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712466.1"
                     /translation="MSTTQAIPKRVRSITQRNRLTAYGFLLPNLIGFVAFTLVPIVIA
                     FGLCFVKWDFANPMEFV"
     gene            complement(354..1619)
                     /locus_tag="VF531_00480"
     CDS             complement(354..1619)
                     /locus_tag="VF531_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7712467.1"
                     /translation="MRKRLSLVFSVLLVAVLLGALGVSAGAAKQETIKFSVWDYSMSP
                     EYKAAIDAFQKANPDIKVDVIEIAAKDYPDKMTVMLAAGEDVDVFAVKDFASYSNYLS
                     RNYLYPLDSFIKKAKVDLKPYGGALNYVKSSGKLMVFPYRSDIYVLYYNKDIFDKAKV
                     AYPTNDMTWAQFRETAKKLTSGEGNDKIWGAYFHSWRSQIQCPILLTTKTTLIDGKYT
                     FLKPAYQLALAMQNEDKSIMSYAEMKTSNANYRGLFESGKVGMSYMGTWFLGSLISDK
                     KAGKHNVNWGIVKAPHWPANKAGSTITGITTMAINAKSSKKDAAWKFINFMGGEQGAK
                     IFAGYGVFPALRTSEVLDVYTSADGFPAGGKDALVTDKTTVEIPPSPYANAIDKTLSE
                     EHELIMTGQKTLDQGLKDMAKRVKEVKED"
     gene            complement(1810..3432)
                     /locus_tag="VF531_00485"
     CDS             complement(1810..3432)
                     /locus_tag="VF531_00485"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7712468.1"
                     /translation="MLRVLIIDDEVLVRVGLKSMLNWGELGFEVCGEATNGRDGLDLI
                     IRLQPDIVLTDIKMPVMDGLEMMRLASESGCRPKYIILSSYDDFLLVKQAMKQGAEEY
                     LIKLDLEPAVLSNTLSAVAGKIRSEHGRSGAEEHFEKVLRENIAMLREVFFKGLINRP
                     FQVQGDLGAKARDLGIELDETILVCALVRMNDPTVLDKYDPQDLRVLEVSFLDTINEI
                     VNDVFKGYTFAWNRGEFVVIYSANPGTPPERYRESSVKMAERWVQVLKQYSNIAVSVG
                     LSKPHSGYAELAQAYLESCRAVQQVFYAGFLSIFFFEDLPETVDNPGRIDLARIRDVL
                     PQAVEMHDLDTIHTVIESIIAVLEEQKMPRGQAFDLCFQVAYLIGGAAGLNETAIQDI
                     IGYQNSLYESIFNLNTLPEIINWFTGLEQRLCLFLTRNEDQKNQRLIAKAKKYIQDHC
                     CEDMGLNEIAAVLNISPGYFSTIFKQLTGVGFIDYVTEKKIEQAKKLLRETDYKIYEV
                     SNLLGYQNPYYFSKVFKKVTGVTPSEFSGKKL"
BASE COUNT          937 a          989 c          797 g          998 t
ORIGIN      
        1 ccacaaactc catgggattg gcaaaatccc acttcacaaa acaaagtccg aaagcgatca
       61 cgatcggcac cagtgtaaaa gcaacaaagc cgatcagatt gggtaataga aatccatatg
      121 cagtcaagcg gttcctttgg gtaatactcc tgacccgttt gggaatagcc tgggttgtgc
      181 tcatgttttt cccaccggta aaaatccagg gtaccggcag tccctccttc tcgcaaaatg
      241 aaatgtacct ttgtaatcat atgccctata tacggatgca tatagggcat atgaaaagct
      301 attgctttac tttagatatt tacggatttc cctcagatca ggagaactga tggtcaatct
      361 tccttaactt ccttgacccg tttcgccata tccttgagcc cttgatccag ggttttttgt
      421 ccggtcatga tcagctcgtg ctcttcggaa agggtcttgt caatcgcatt agcatacggg
      481 ctgggtggaa tctccaccgt tgttttatcc gttaccagag catctttacc tccggcaggg
      541 aaaccgtcgg cagaagtgta aacatccaat acttccgaag tccgtaaggc cgggaacacg
      601 ccgtagcccg caaaaatctt ggcgccttgc tcgccgccca tgaagttgat gaacttccag
      661 gccgcatctt ttttggatga tttggcgttg atcgccatcg tagtgattcc ggtaatggtt
      721 gaaccggctt tattggcggg ccaatgcggg gctttaacaa taccccagtt cacattgtgc
      781 ttaccggcct ttttgtcgct gattaacgag ccgaggaacc aggtccccat atagctcata
      841 ccaactttac cgctttcaaa caatccccgg tagttggcgt tggaggtttt catctccgcg
      901 taactcatga tcgacttgtc ttcgttctgc atggctaagg ccaattgata agcgggtttc
      961 aggaaggtat acttcccgtc gatcaacgtg gttttggtgg tcaacaaaat cgggcattgg
     1021 atctggctcc gccaggaatg gaaataggcg ccccaaatct tgtcgttccc ctcgccgctg
     1081 gtcaatttct tggcggtctc acggaactga gcccaggtca tatcgttggt gggataggca
     1141 accttggcct tgtcgaagat atccttgttg taatagagaa cataaatatc actgcgataa
     1201 gggaatacca tcagtttacc actggattta acataattta atgcgccgcc gtatggtttc
     1261 aaatccactt tggctttttt tataaacgag tctaagggat ataggtagtt ccggctcaga
     1321 taattggagt aactcgcaaa atccttcacc gcaaatacat ccacatcctc accggcggcc
     1381 agcatcactg tcatcttgtc gggataatcc ttggcggcaa tctcgatcac atcgactttg
     1441 atatccggat tcgccttttg gaatgcatcg atggccgcct tgtactccgg agacatactg
     1501 taatcccaaa cagagaactt gatcgtctct tgtttggccg ccccggcgct gacgcccaaa
     1561 gcgccaagga gaaccgctac cagtaaaacc gagaagacca gactcaaccg ttttcgcatc
     1621 caatcattcc tccttttaaa gttctgccct tattatagat ttgttacggg atgattaaaa
     1681 tgtctttaaa cgcaataatt tgtaatatta tccggacaag atggttatcc aaaatttacc
     1741 ataatacttt ttttcatttt cagttacttt tttcatcccc cgtgttccct tgtttgccca
     1801 ccctcgtttt taaagcttct taccggaaaa ctcactggga gtcacgccgg tcaccttttt
     1861 aaagaccttg ctaaaataat aggggttctg atatcccaag agattcgaaa cctcataaat
     1921 cttataatcc gtttcccgaa gcagtttctt ggcttgctcg atctttttct ccgtgacata
     1981 atcgatgaag ccgaccccgg tcaattgttt aaagatagtg ctgaaataac cgggactgat
     2041 attcaatacg gcagcgatct cgtttaaacc catatcctca cagcaatgat cctggatata
     2101 cttttttgct ttagcgatca gtcgctggtt cttttgatct tcattccggg tcaggaataa
     2161 gcacaatctt tgttccagtc cggtgaacca attaataatc tccggaagcg tgtttaaatt
     2221 gaaaatgctt tcgtacagac tattttgata cccgataata tcctggatgg ccgtttcatt
     2281 gagaccggct gccccgccaa tgagatacgc cacttgaaaa cagagatcga atgcttggcc
     2341 ccggggcatc ttctgttcct ccaaaaccgc gatgatactc tcaatgactg tatgaatcgt
     2401 atccaggtcg tgcatctcca cggcttgagg aaggacatcg cggatcctgg cgagatcgat
     2461 ccgtcccgga ttatcaactg tttccggaag atcttcaaaa aagaatatcg acaggaaacc
     2521 tgcataaaaa acctgttgca ccgctctgca gctttccagg taggcctggg caagctccgc
     2581 ataaccggaa tgcggtttac tgagaccgac ggatactgca atattggagt actgttttaa
     2641 aacctgaacc cagcgctccg ccatcttgac cgaactttca cgataacgct ccggcggtgt
     2701 tcccggattg gcggaataga tgactacaaa ctccccacgg ttccaagcaa aggtatatcc
     2761 cttaaaaaca tcattcacga tctcattgat ggtatccaag aaggaaacct caagaacccg
     2821 aaggtcctgg ggatcatatt tatcaagcac tgtcggatca ttcatccgca ccagagcaca
     2881 gacaagaatg gtttcatcca gttcaattcc cagatccctc gccttggcgc ccagatcccc
     2941 ctggacctgg aacggccggt tgattagtcc cttaaaaaag acctccctaa gcatggcgat
     3001 attttcccga agcacctttt caaaatgttc ctccgctccg gaacggccgt gctcgctccg
     3061 gatcttcccc gccacagcgc ttagggtatt ggataaaacc gccggttcca aatcaagttt
     3121 gatcaggtat tcctccgccc cctgcttcat ggcttgcttg accaacaaaa aatcatcata
     3181 actgctgagt atgatgtact taggccgaca tccggattcc gaagccagcc gcatcatttc
     3241 caatccatcc ataaccggca ttttgatgtc ggtaagaaca atatccggct gcaaccggat
     3301 gatcaggtcc agtccgtccc ggccattggt tgcctctccg caaacctcaa accccaactc
     3361 cccccagttg agcatggatt tcaaccccac ccggaccaga acttcatcat caataatcag
     3421 cactctcagc atttactaat cacccgtttc tttaggcatg aggcgtcgcg tcctgcggga
     3481 cgcgcggcaa ctcaccccgc gccatcctta cgctttcagt tttaccctaa accggggctg
     3541 tctcaaaagt ccgatttgtc attccgtcca cccggggtta aaaccccggg ctaagttaaa
     3601 aaccattcta aagcccgtta aaacgggctg ggaagaactg gatgtattac ccagccccat
     3661 tcatggggct tgagatgatt tttccgggcg tagccaggtc ctttagggcc tggtggggat
     3721 a
//
LOCUS       DASVMB010000016         5708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_76570,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000016 DASVMB010000000
VERSION     DASVMB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76570"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF531_00490"
     CDS             complement(<1..486)
                     /locus_tag="VF531_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7712469.1"
                     /translation="MPKSPKKVYYCENCGHQEHKWVGRCPACHEWGTMQEEIEELQTA
                     DELETLSSFQQPQVLNQVRIDSTIRMSTGIGEFDRLLGGGIVLGSVLLLVGDPGIGKS
                     TLSLITAGNAARDGVVLYVSREESAEQTKIRADRLGTFPDTLYVISETNLELVQQHIA
                     NL"
     gene            complement(779..1570)
                     /locus_tag="VF531_00495"
     CDS             complement(779..1570)
                     /locus_tag="VF531_00495"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX7712470.1"
                     /translation="MKEAQIGIFGGSGFYSLLDRVEEVAVQTPYGAPSDRIAIAEIAG
                     KKVAFLPRHGKNHQHPPHMINYPANLWAMKSLGVRQIIGPCAAGSLQPEINPGDFVVI
                     DQFVNRTWGRKDTFFDGPITTHLSGADPYCRRLREQAILSAKQLGITVHETGTVVVVQ
                     GPRFSTRAESREFKRNGWEVINMTQYPEGWLARELGICYVNIALITDYDEGLEGNPDI
                     KPVTHEQVLQVFESNNERLRELLFSMIRDLPALPDCECSKSGGEI"
     gene            complement(1811..2026)
                     /locus_tag="VF531_00500"
                     /pseudo
     CDS             complement(1811..2026)
                     /locus_tag="VF531_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
     gene            2181..3335
                     /locus_tag="VF531_00505"
     CDS             2181..3335
                     /locus_tag="VF531_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7712471.1"
                     /translation="MNLADLIYGYQKKVPAKGGNKRYINLDNAASTPPFKAVMELICR
                     EAEWYSSVHRGSGFKSKYSTAQYEASRQVIADFIGADLRHDTVIFTKNTTDSINKVAH
                     YLPNIPGEYVIYSGMEHHSNELPWSQVPHICIGVKENGIDLDQLEQALRLESGRVKLL
                     AVTGASNVTGHTPPIHTLAELAHQAGAKILVDGAQLIPHRQINLFPPSDPRHIDFLAF
                     SGHKIYAPFGAGVLIAPRSLFQKNPPSQVGGGTIIGIGPEGIIWAEAPDVEEAGSPNI
                     LGAMAIAESCRVIQRIGWDPITRHETELLEYALKCLEELPELEIYNREKQNRVGVISF
                     NIRGIPHAIVAQHLAERGIGVRSGCFCARGYVQSLLDYLQKKFKSCCTGS"
     gene            complement(3469..4002)
                     /gene="pgsA"
                     /locus_tag="VF531_00510"
     CDS             complement(3469..4002)
                     /gene="pgsA"
                     /locus_tag="VF531_00510"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7712472.1"
                     /translation="MSLANWITALRIILAPLCVGFLFWDIPGRDYLAAIVFIIAGLTD
                     GLDGYAARSRKEVTSFGKSFDPFADKVLIILTLVVLERLDRVPLVPVLIIIIRELFIT
                     VLRYFPGRKGLVVAASPWGKLKTCTQIIAVALIMLRLPYSLWFLWAAVIFTIWSGLDY
                     LWRWRSAFGIKPKDAVL"
     gene            complement(4172..5488)
                     /gene="rimO"
                     /locus_tag="VF531_00515"
     CDS             complement(4172..5488)
                     /gene="rimO"
                     /locus_tag="VF531_00515"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7712473.1"
                     /translation="MPKLAVVSLGCAKNLVDTEIMLGQLVGEGWEVTRNFADAELILV
                     NTCGFIEEAKAESIRNILEFAEYKKPDCGSCRKLVVAGCLVERYSQELAKELPEVDYW
                     IGLGEIDRIAGLVQEKSSGPKVGGSPFLNDENLPRYQVTLSHTAYVKIAEGCNHRCAY
                     CAIPNIKGGFRSRRPESVVREISRLVENGVREINLIAQDITMYGRDLPEKIDLKKLLE
                     QIVSQAKPSWIRLLYAYPSGISRDLLEFIAAEPRVCKYLDLPLQHINGRILKLMRRSD
                     PPERIRELLTTIRTVVPELTLRTTLIVGFPTETDGEFEEMLDFVKEGHFQHLGVFAYS
                     PEEDTPAYDYKPQIPEKVRTERKQRLLAAQQPISARFLAELVGRELPILIDRVTPDGG
                     AIGRTPSLAPDVDGVVYLNQFHGKPGEIRSGLITASDAYNLYAKLD"
BASE COUNT         1520 a         1503 c         1283 g         1402 t
ORIGIN      
        1 aagattggca atgtgttgct gaaccaattc gagattggtt tcggaaataa cataaagcgt
       61 atcaggaaaa gtacccaacc ggtcggcacg aatctttgtc tgttcggccg attcctctcg
      121 gctcacgtaa agcaccaccc catcccgggc ggcgtttccc gctgtaatca ggcttaaggt
      181 actttttccg attccggggt cacccaccaa aagaagcacc gaacccagaa cgatgccccc
      241 tcccaataac cggtcgaatt caccaatccc ggtactcatc cggatggtac tatcaatccg
      301 aacctgattc aatacttggg gttgttgaaa gctactcagg gtctccagtt catcggcggt
      361 ttggagctcc tcgatctctt cctgcatggt tccccattca tggcaggccg gacaacgacc
      421 tacccacttg tgctcctgat gaccgcagtt ttcacaataa taaactttct tgggtgattt
      481 cggcattgtt tatcccctta tctatgcaga caatgaatcc ctaaagcttg ggtagggttc
      541 cttacaatat acaaactccc ccatgaaatg attccacctc aagggcagcg ggagttggaa
      601 taattgctaa actatttcga ttccagtcgg cgaattccta tttgaaagaa gataaaataa
      661 aaaatcggca gtcaactgcc gatcaccaaa aacaatcttt aaattacctt ctttagagag
      721 aatttaaatc ccaaacgcca cttgagcttt ggtttaaact ccgtaaaata aaacagcgtt
      781 aaatttctcc tccggatttg ctgcactcgc aatctggaag ggccggtaaa tcccggatca
      841 tagaaaataa aagttcgcga agccgttcat tattactttc aaagacctgt aaaacctgct
      901 catgggtgac aggtttaatg tctgggttac cctctaagcc ctcatcgtaa tcggtgatta
      961 gcgcgatatt gacataacag atccctaact cccgggcaag ccaaccttcc ggatactggg
     1021 tcatgttaat gacttcccag ccgttacgtt taaactccct gctctcagcc cgggtagaaa
     1081 aacggggacc ctgcaccacc acgacagtcc cggtttcatg cacggtaatt cccaactgtt
     1141 tggcagagag gatagcttgc tcacgaagtc tccggcaata gggatccgca ccagagagat
     1201 gggtcgtgat tggcccatca aaaaaagtat cctttcgtcc ccaagtacgg ttgacgaact
     1261 gatcgatcac cacaaaatcc ccggggttga tctccggttg caggctgccg gcggcgcaag
     1321 gcccaatgat ctgtcggact cccagagatt tcatggccca gagatttgcc gggtaattga
     1381 tcatgtgcgg tggatgctga tgattctttc catgacgcgg taaaaacgcc actttctttc
     1441 cggcaatctc ggcgatggcg atccggtcac ttggagctcc gtatggggtc tgtacagcaa
     1501 cctcctccac cctgtcaagt aaagaataga agccggagcc cccgaagatt cctatttgag
     1561 cttctttcat aagccattcc cccttcatct ttcgctgttt ttcgcttttt atcaaaaaaa
     1621 ttcctgcccc aaaacggcag gaatttttaa agcggtacct attgtgggac cgaactcacc
     1681 aacttaagta ttaaacatat ttatctaagt tgttcaaatt caagttcggg gtggttggaa
     1741 ccttatcctg ttgcacctgg ctgctgctct gtaatccagc cttaaatcca gcctgaacgt
     1801 tagcaaaagc ctgttcggca atggaacgtt cggccgcttc gatcatccgc cgaaccatgt
     1861 taccacccac ggcgccacaa tcccgggaag tcatatagcc ccagtaatca ccgacaatcg
     1921 gagaggtatc gatattcagc tccttggata tctcatactt aaaattctga agagctttcg
     1981 tcgcctcttg gactacataa ctattccttt tttggccagt acccattttt ttcactcctt
     2041 tcgtagtgat ctgagtctaa tttgcccagc tatatgtgga taaaaaccaa aaaactctgg
     2101 aatatttcaa ggagaatccc aagagtgcca ggatagtcaa ttggcctact tgcctcatat
     2161 gataaagtga ggtgctgcct ttgaatctgg ccgacttgat ttacgggtat caaaaaaaag
     2221 ttcccgccaa gggcggaaac aaacgatata ttaatctcga taatgccgcc agtaccccgc
     2281 cctttaaggc agttatggaa ttaatctgcc gcgaggctga atggtactct agcgtacacc
     2341 ggggatcggg cttcaagtcg aaatactcga ccgcccaata tgaagcatca cggcaagtta
     2401 ttgccgattt catcggtgcc gatctccgtc atgacaccgt tatttttacc aaaaacacca
     2461 ccgattccat aaataaagtc gctcattacc ttcccaacat ccccggcgaa tacgtgattt
     2521 attccgggat ggaacaccac tccaatgagc tgccctggtc gcaagtaccc catatttgca
     2581 ttggagtgaa agaaaacgga atcgacctgg accaattgga gcaagcgttg cgactcgaaa
     2641 gcggtcgggt taaacttttg gccgtaaccg gcgcttcaaa tgtcaccgga cacactccgc
     2701 ccatccatac cctggctgaa cttgcacatc aggccggcgc taaaatcctg gtggatgggg
     2761 cgcagttgat accccatcgt cagatcaacc ttttcccccc ttccgacccc aggcatattg
     2821 actttttggc gttttccggc cataaaatat atgctccctt cggtgccggt gtactcatcg
     2881 ctccgcgttc cctgtttcaa aaaaaccctc ccagccaggt gggaggggga acgatcatcg
     2941 gaatcggacc ggaaggtata atctgggctg aagcgcccga tgttgaggaa gcgggttccc
     3001 ccaatatatt gggggcaatg gcgatagccg aaagctgtcg agtgattcaa cgaatcgggt
     3061 gggatcccat tacccgtcat gaaacggagt tactggaata tgccttaaaa tgcctagagg
     3121 aactaccgga actggagata tataacaggg agaaacaaaa ccgggttggg gttattagtt
     3181 ttaatattcg gggaattccc cacgctattg tagcgcaaca tctggcggaa cgtgggattg
     3241 gcgttcggag cggttgtttt tgcgcccggg gctatgtcca aagtttgttg gattatctgc
     3301 aaaagaaatt caaatcttgc tgtaccgggt cctaaaagga gatattggcc acgaaaccat
     3361 gccaggcatg gcccgattga gtttcggctg ttataatcag atatcggaag tggatatcct
     3421 catcctggct cttaaggagt taattaccga ttgtactaaa atatagtctt acaatacggc
     3481 gtctttcggc ttgatcccga aggcactgcg ccagcgccac aggtagtcga ggcctgacca
     3541 gatggtgaag atcacagcag cccataaaaa ccaaaggctg tagggcaacc ggagcatgat
     3601 taaggcaact gcaattattt gagtgcaagt ctttaactta ccccagggac tggcggcgac
     3661 gactaaaccc tttcgacccg ggaaataccg caaaacggtg atgaacagct cacgaatgat
     3721 aatgatcaat acgggtacca gaggcacccg gtcgagccgc tccaaaacca ccaaagtcag
     3781 gatgatcaaa actttatcgg caaaaggatc gaaggacttg ccgaaactgg tgacttcctt
     3841 gcgggagcgg gcggcgtagc catccaaacc gtcagttaat ccggcaataa tgaagacgat
     3901 cgcagccaaa taatcccggc cgggaatatc ccagaataaa aacccgacgc acaacggggc
     3961 taaaatgatt cgaagcgcgg ttatccaatt cgccaggctc atcgacttgt cctccagcaa
     4021 taacttccgg ggtaaggcac tctagccccc atacacctgc actgatatgg ttaattaatc
     4081 caacgattcg gcatctttct tgaaattcct ttatcctgaa atgggctttc aaaattttta
     4141 aagaaagatc cggatggata tatttgaatg gctaatccaa tttggcatat aaattatagg
     4201 catcgcttgc ggtgatcaac cccgaacgga tctcaccggg tttaccgtgg aattgattca
     4261 ggtaaaccac gccatccaca tccggggcca gacttggggt tcgaccaatg gctcctccgt
     4321 ccggggtgac ccgatcaatc aagatgggta actcccgtcc gaccagttcc gccaggaacc
     4381 gagcagagat aggttgttgc gccgcaagca gtctttgttt acgctccgtc ctgaccttct
     4441 ccggtatctg cggtttgtaa tcgtaggcag gggtatcttc ctcgggactg taggcaaata
     4501 cccccaggtg ctgaaagtgc ccttctttaa caaagtccag catctcctcg aattccccat
     4561 cggtttcggt cggaaatccc acaatcaacg tagtccgtaa ggtcagttcc ggtacaaccg
     4621 tacggatcgt cgtgagaagc tcccggatcc gttccggggg atcggagcgc cgcatcaact
     4681 tcaaaatgcg tccgttgatg tgttgcaggg ggagatccag atacttacaa accctgggct
     4741 cagctgcaat aaattccaat agatcacgac taatcccgga agggtatgcg tagagcaaac
     4801 gaatccatga tggcttggcc tgagatacga tctgctccaa aagctttttg aggtcgattt
     4861 tttccggtaa atcccggcca tacatcgtaa tatcctgcgc aattagattg atctcccgaa
     4921 ccccgttctc aaccaaacgg ctgatctccc gaacaaccga ctccggtcgt ctactccgga
     4981 acccgccttt gatattgggt atggcgcaat acgcacagcg atgattgcag ccctccgcga
     5041 tcttcacata cgcggtatga gataatgtca cctgataacg gggaaggttt tcatcattga
     5101 gaaagggaga gcctccaacc ttaggtccgg atgatttctc ctgaactaaa ccggcaatcc
     5161 ggtcgatctc tcccaaaccg atccagtaat cgacctcggg tagctccttg gccagttctt
     5221 gggaatacct ctcaaccaga caaccggcaa ccaccagctt gcggcaggag ccgcaatccg
     5281 gctttttata ctcggcgaac tccagaatgt tccggatcga ttccgcctta gcctcctcaa
     5341 tgaatccaca ggtattaacg agaattaatt cggcatccgc aaaattccgg gtaacttccc
     5401 atccttcccc gaccagttga ccgagcatga tctccgtatc taccaaattt ttggcgcaac
     5461 ccaaagatac cacagccaat ttgggcatat atcctacctc catgttgcca atcgtacaaa
     5521 taaaactcca ccggaccgga ttaaaaataa atcgattata ttattcacgg acgccgggtt
     5581 aaaaaacaaa tcccttacga taaaacatcc gaatacagat aaccatagat ccgtttaaga
     5641 aacatgatcc gtagcagata ttttctggtt tccgggaaag gtatctgctc aatccggact
     5701 ttattacc
//
LOCUS       DASVMB010000017        10108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_591650,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000017 DASVMB010000000
VERSION     DASVMB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591650"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VF531_00520"
     CDS             <1..964
                     /locus_tag="VF531_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEX7712474.1"
                     /translation="QAAIDSVPQNNCKQVQIQVKNGVYREKIRVTSPWISLIGENPLR
                     TLIAFDDAAKKPLPGGEPMNTFNSYTIYIGGHDFTAEGLTFENSAGDGRIVGQAVAVY
                     ADADRITFKNCRLLGHQDTLFTGPLPKNPVPKGLNLVHPALGSGDAEYTGEIRQYYRD
                     CFIRGDIDFIFGSATAVFDHCEIFSNDRGEPVNGYITAGSTSPRQEFGYVFTDCRLTG
                     AAGPHTVYLGRPWRDFAKAAFINCSMGEHIIPAGWDNWDLPEREKTVNYCEFGSTGPG
                     AALADRVKWSKQLTAEQVKKYAALNVLSGSDGWEPDKDADCLKA"
     gene            complement(1157..1816)
                     /locus_tag="VF531_00525"
     CDS             complement(1157..1816)
                     /locus_tag="VF531_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7712475.1"
                     /translation="MDKEKLNSRCRVYTFHDIPECLTHVFLINGKRHVFVIDTYCGPD
                     CMVPILNDIATMAQGKEIVVINTHYHWDHVWGNCAFRDAEIIAHSLCKTKMEQEWERM
                     MTSQQQFIRGKVEPKFPTITFNERLVFEEEGITLEHTPGHTSDSITVYSSGDRIVFVG
                     DNLERPKIYLEEQNVDTYVQTISRYRELENQRIMAGHSLDLSCTDIQANLESLLELKG
                     K"
     gene            complement(1818..3062)
                     /locus_tag="VF531_00530"
     CDS             complement(1818..3062)
                     /locus_tag="VF531_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX7712476.1"
                     /translation="MLHIAKKVNRLQREELRSVKNQFHQELRDNILRFWTGHAVDVEN
                     GGFHGYITNDLKVTKNAPKASVLNARILWTYSTAYRIFKTDEYLTMAKRAYDYLADHF
                     IDPEYSGVYWMLDYKGKPIDSKKQIYAIAFAIYGLAEYFQATGDQQSLEKAIGLYQAI
                     EEHSYDPENQGYFEALSRDWGLLADMSLSPKDLNVKKSMNTHLHILEAYTQLLRVWKD
                     EALAGKLRELIRITMDRILNRKKDRFQLFFDECWHSQADIVSFGHDIEGSWLLYEAAE
                     VLGDPAVLAEVKPIALRMAENVLEHGIDTQDGGLFYEAEGGRFTDTNKDWWPQAEAVV
                     GFCNAYQLSGEECFLDASFAVWDFIKRRIVDKQYGEWFWGVSKDGKVQDGREKVGPWK
                     CPYHNSRMCFEMMQRLDEIRGG"
     gene            complement(3117..3797)
                     /locus_tag="VF531_00535"
     CDS             complement(3117..3797)
                     /locus_tag="VF531_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HEX7712477.1"
                     /translation="MAISASDVKQITAELGADLCGIAPIDRFSDAPRGFHPHDVLPEC
                     QSVIVFARRFLTSSLKAKSTVPYTTVRNELSAHMNWMGVRMAQMLEDGGMTAVPANTT
                     GPDEYDQETGRYRGIISLKHAAMNAGLGKIGKNTLLVNNRFGNMIWLDAVLVSEPLAP
                     DPLADYEVCNPKCRLCIESCPVGALGGELMNQKACKHFAFGELNGGEWRIKCFECRKV
                     CPNSTGLR"
     gene            complement(3821..4900)
                     /locus_tag="VF531_00540"
     CDS             complement(3821..4900)
                     /locus_tag="VF531_00540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7712478.1"
                     /translation="MEENSAIDSSNRRYEKFPAFTVHAAQETNRQIITPEHPGLYYIG
                     RIDRKDPGAPRFIWQGTELRFSFSGRRLGLRFSDAWGQNFYNVIIDGRVILLQLVEGG
                     SHDYLLDEILPEGWHEVIIFKRTEAMFSNAVFRGLILERGTEMGSKPEPLPLAIEFYG
                     DSISAGACNEDPAADQYDDYSTHNNYRSYGAMTARNLNAEYVNIAVSGTGICYSWNPV
                     LMPEVYDKLYPENTSERYDFSGRIPDIVLLNLGQNDYGYPNSLGKPFPSDFTEKYTEL
                     VRNLRGLYPDARIICAIGGMSAYRESPDLQAAFKKAVDILKSADERIYSFVFTAYTDN
                     HPRVDTHAKLAAELTPLIQKVVLPE"
     gene            complement(5014..5970)
                     /locus_tag="VF531_00545"
     CDS             complement(5014..5970)
                     /locus_tag="VF531_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7712479.1"
                     /translation="MNREERIGKVIRTMIAMERRAWEQGVAAQALLELGETDLVILLA
                     KDAVVNQLKDGRLGLNGDRIPVADPAANGEPVLFAAKATGDDALHRAAQRMLEYLLYQ
                     APKTRDGIIYHNQIENRIWVDAFYMVPPFLAVAGHPDEACKQILGYRRVLWDPQKQLF
                     HHIWDEDLQQFERKLFWGVGNGWAAAGMTRVIRALPDSMTKEKNMLAGFVKEAIDGCL
                     KFQRSDGLFHDVLDDPATFVETNAAQMLSYSMYRGVQGGWLNPSYQTQADRIREAVYQ
                     KVDQFGLVQGVCGAPNFDRPGTATEGQAFFLLMEAAANDMNH"
     gene            complement(6034..6813)
                     /locus_tag="VF531_00550"
     CDS             complement(6034..6813)
                     /locus_tag="VF531_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712480.1"
                     /translation="MERKERLQQRLSEIAESLKTSENALALMGLGSAGQEVHRMDNYS
                     DLDFFAIVRDGYKQRYIENTDWLERIRPVVFKFRNTRDGYKIMFDDGIYCEFAVFETP
                     ELADIPYAPGRIIWKDEGFDTKLSIPQHTGAPDWKPESIEWLLGEIITNIYVGVCRYY
                     RGEKLAAYRGVQGNAFNLFIELIALSCAADEKVCRDIYSKERRFEQRYPGMEPVLARI
                     LPGYEHTVNAARAMLIYLDENYSINRYMRTLILNICENGIE"
     gene            complement(6871..7512)
                     /locus_tag="VF531_00555"
     CDS             complement(6871..7512)
                     /locus_tag="VF531_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712481.1"
                     /translation="MLKTEHVEVFNLRGALRGMRNPMASWHLSDSIYNEQSGALLTLG
                     ENDRKLALRLVTAGSDHGKFIRQIFVSMDITAPDYWWKEMDTYKVGTVANSTSTMHKL
                     TSRSLTADDFSWDEVTSFKKGLLEHLNELISVYKEAVQKERPEEANRLWRQLIQDLPM
                     SFNYTRTWTGNYANLRNMYHARKVHKLIEWHAFCRTIEELPFSELITTINEPK"
     gene            complement(7545..8060)
                     /locus_tag="VF531_00560"
     CDS             complement(7545..8060)
                     /locus_tag="VF531_00560"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7712482.1"
                     /translation="MQTNVVLIGMPGAGKSSVGVLLAKALGMSFVDTDLLIQEKSGRL
                     LQTIIDQDGVERFLAMEEAVILQLERENSVIATGGSAIYSEATINHLKRNGKLIYLKL
                     KYEEIESRIKNMSSRGIAMGKGQRLIDLYNERVVLYEKYADIIVDCSGTVIESVVGKI
                     SNRIKANLARS"
     gene            complement(8091..8462)
                     /locus_tag="VF531_00565"
     CDS             complement(8091..8462)
                     /locus_tag="VF531_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7712483.1"
                     /translation="MIHFEKNRKYIVIQDRESDYPDPISLKPNDRVEVVGEYSGKEAW
                     PDWIRCRNQGNEGWVPRQIIQIQGNAGIILEEYSASEMRIGKNDIINGEKEVNGWIWG
                     TNQTNGEVGWVPLEYISETDD"
     gene            complement(8617..9252)
                     /locus_tag="VF531_00570"
     CDS             complement(8617..9252)
                     /locus_tag="VF531_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712484.1"
                     /translation="MPVSVMDKLASSLGRNDEVPNQELARELCRTGDRNSIHELVQLL
                     FSGSAAIQNDSIKVLYEIGEVQPELIADDVEAFIRLLTSKNNRLIWGAMTALSTIAPI
                     RAYKIYENVDLIFKAIKQGSVITVDNGVSVLAKVGSVKNEYETVILPFLLDHLASCKP
                     REVAQHSERSMIIANTGNKVHFIQVLEQRMQDLTPAQQVRVKKVIKKVGGL"
     gene            complement(9291..9854)
                     /locus_tag="VF531_00575"
     CDS             complement(9291..9854)
                     /locus_tag="VF531_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7712485.1"
                     /translation="MIRTRIKDIDFVFETHETVFSPKSIDRGTLAMLSVIEFQETDKV
                     LDLGCGYGVVGILAAKLIGTEKVYMLDIDEGAVGLAKRNAELNGVPGIRILQSDGFGN
                     LDEKDFSLILSNPPYHVDFSVPKLFIEKGFNRLAVGGRMVMVTKRKEWYQNKLTGIFG
                     GVRIWEIDGYYVFMSVKKSGTYAKAKK"
     gene            complement(9847..>10108)
                     /locus_tag="VF531_00580"
     CDS             complement(9847..>10108)
                     /locus_tag="VF531_00580"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002755070.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX7712486.1"
                     /translation="TRRADILVAAVGKPKFIQGDWVKKGAVLVDAGYNPGNIGDIDLE
                     HAVPNCSAYTPVPGGVGPMTIVSLIRQTVEAAEKKYTANRND"
BASE COUNT         2464 a         2787 c         2205 g         2652 t
ORIGIN      
        1 gcaggcggcg attgattccg taccgcagaa caactgcaag caggtgcaga tacaggttaa
       61 aaacggagtt taccgcgaaa agatccgcgt cacttcgccc tggatcagcc taatcggcga
      121 gaatccgctc cggaccctaa tcgcctttga tgacgccgcc aaaaagccgc ttcccggcgg
      181 agaaccgatg aacactttta actcctatac catctatatc ggcggccatg attttaccgc
      241 agaaggctta acttttgaaa actccgccgg cgacggccgg attgtcggcc aggcggtagc
      301 ggtatacgcc gatgcggatc ggattacctt caaaaactgc agactcttgg gtcatcaaga
      361 taccttattc acgggccctc tccctaaaaa ccccgttccc aaaggattaa atctggtcca
      421 ccccgctttg ggttccgggg atgcggagta caccggggag atccgccagt attaccggga
      481 ctgtttcatc cggggggata tcgatttcat cttcgggtcg gccaccgcgg tattcgatca
      541 ctgcgagatc ttctcgaacg accggggtga acccgtcaac ggctatatca ccgccggatc
      601 gacctcgccc cgtcaggagt tcggttacgt cttcaccgat tgccggctga ccggggctgc
      661 cggaccccat accgtttatc tgggcaggcc ctggcgagac tttgccaagg cagccttcat
      721 caactgctca atgggcgaac atatcatccc ggcaggctgg gacaattggg atttgcctga
      781 acgggaaaaa accgtcaatt actgcgaatt tggctcaacc ggtccgggcg ccgccctagc
      841 ggacagagta aaatggtcca agcagttaac ggcggaacaa gtgaaaaaat atgccgcttt
      901 gaacgtcctg tccggcagtg acggctggga acccgataag gatgcagact gtctcaaggc
      961 atgagaaccg aagggagccc tcattctatc tttctcccag agggaaaaac gaacggctta
     1021 gtcaagttgc cacttttaag aaacgaacgg ctctctggca ttctaagtgc ccggcaccgt
     1081 ccgctttcaa gaagtatgcc gataccccgc tgatggtcaa aatctaccgt attgcaatga
     1141 acagttttaa taaaatttac ttccctttca actccaataa actttctaga ttcgcctgga
     1201 tgtcagtaca agataaatca aggctatgtc cggccatgat ccgctgattc tccaattcac
     1261 gatatctgga gatggtttgg acatatgtat ccacattctg ttcttccaga tagatcttgg
     1321 gcctttccag gttatcgccg acaaagacga ttcggtcacc gcttgagtaa accgtaatgc
     1381 tgtcgctggt atggcccgga gtatgttcta gcgtgatgcc ttcttcttcg aagaccaacc
     1441 gctcgttaaa tgtgatagtc ggaaacttcg gctcgacttt accccggatg aactgttgct
     1501 gcgatgtcat catccgttcc cactcctgtt ccattttggt tttacataag gaatgggcga
     1561 taatttcagc atcccgaaag gcgcagttcc cccagacatg atcccagtgg tagtgggtgt
     1621 ttatcaccac aatctccttg ccttgcgcca tagtggcaat atcgttcaat atgggaacca
     1681 tacaatccgg tccgcaatag gtatcaatga cgaatacatg ccgcttcccg ttgatcaaga
     1741 atacatgggt gaggcactcg ggtatgtcat gaaacgtata gaccctacat ctggagttta
     1801 gcttttcttt atccatgtca accacctcta atctcatcca gccgctgcat catttcaaaa
     1861 cacatccggc tgttatggta aggacatttc caggggccta ccttttctct gccgtcctgc
     1921 accttgccat ctttggaaac tccccagaac cactcgccgt attgtttatc gacgatccgg
     1981 cgtttgatga aatcccatac agcgaaggat gcatccaaaa agcactcttc tccggataac
     2041 tgatacgcat tacagaaccc aaccaccgcc tcggcctgag gccaccagtc tttattggta
     2101 tcggtaaagc gaccaccctc cgcttcgtaa aaaagaccgc cgtcttgggt gtcgatgccg
     2161 tgttccaata cattctccgc catccggagg gcaattggtt ttacctcggc taaaaccgcg
     2221 ggatcaccca agacctcggc cgcctcgtat aagagccagc tcccttcgat gtcatggccg
     2281 aaggatacga tatccgcttg agaatgccag cattcatcga agaagagttg gaaccggtct
     2341 ttcttccgat tcaagattcg atccatcgtg atccggatca gttctctgag ttttcctgcc
     2401 aacgcctcat ccttccaaac ccgcaaaagt tgagtataag cctccagaat atgaagatgg
     2461 gtattcatcg atttctttac attcaagtcc tttgggctca ggctcatatc ggccaggagt
     2521 ccccagtcgc ggctcaaggc ctcaaaataa ccttggttct ccggatcgta gctgtgttcc
     2581 tcgatggcct gataaagccc aatcgccttc tcaaggctct gctggtcccc ggtggcctgg
     2641 aaatattccg ccagaccgta gatagcaaag gcaatggcat agatctgttt tttggagtct
     2701 atgggttttc ctttatagtc cagcatccaa taaactccgg aatactccgg atcgataaaa
     2761 tgatccgcca aataatcata ggcccgcttg gccatggtca gatactcatc cgtcttaaaa
     2821 atgcggtacg cggtagaata agtccacaat atcctggcat tgagtacact agccttcgga
     2881 gcatttttgg taaccttcag atcattcgtg atataaccgt gaaagccccc gttttcaaca
     2941 tccaccgcat gtcccgtcca aaaccgtaaa atattgtccc gtaactcttg atgaaactga
     3001 ttctttaccg atctcagctc ttctctttgc aaacgattca ccttcttcgc aatatggagc
     3061 aaatccttct tccttttcct ttagaaacct gattgcttta acgaacggtg aagatcttat
     3121 ctcaatccgg tagaattggg acagaccttc ctgcactcaa agcacttgat ccgccattcg
     3181 cccccattga gttctccaaa cgcgaaatgc ttgcaagcct tttgattcat caactcgccg
     3241 ccgagcgccc ccacaggaca ggattcgatg cacaacctgc atttgggatt gcaaacctcg
     3301 taatccgcca gcggatcggg agccagcggt tcagatacca acactgcatc gagccagatc
     3361 atgttgccga aacggttgtt cactaaaagg gtattcttac cgatcttgcc gagaccggca
     3421 ttcattgccg catgttttaa agagatgatc ccacgataac gccctgtttc ctgatcatat
     3481 tcatcgggac cggtggtatt ggcgggaact gccgtcatgc ctccatcctc aagcatttga
     3541 gccattctga ctcccatcca attcatgtga gccgataatt cattccgaac cgtagtatag
     3601 ggtacggtag actttgcctt taaggaactc gtaaggaacc tgcgggcaaa cacgatgacc
     3661 gattgacatt ccggcaatac atcatgggga tgaaagcccc gcggggcgtc gctgaaacgg
     3721 tcaatgggag caattccgca caaatcagcg cccagttccg ctgtaatctg cttcacatcg
     3781 gaagcggaaa tcgccatttc tttcactcct ataataaaag ttattccggt aaaacgacct
     3841 tctgaatcaa cggggtcaat tccgccgcca gcttggcatg ggtatccact ctgggatgat
     3901 tatccgtgta tgcggtgaat acgaaggagt aaatccgctc atcggcggat ttcaatatat
     3961 cgaccgcctt cttgaaggcg gcttgaagat ccggggactc acggtaggcg gacatgccgc
     4021 cgatagcgca gatgatccgg gcatcggggt acaacccccg aaggttgcgg acaagttcgg
     4081 tatacttttc cgtaaaatcg gagggaaacg gcttccccag agaattcggg taaccatagt
     4141 cattctggcc cagattgaga aggacaatat cgggtattct ccccgaaaaa tcataacgct
     4201 cgcttgtatt ctcgggataa agcttatcat aaacctccgg catcaacacc ggattccaac
     4261 tgtagcagat gccggtgccg cttacggcga tattgacgta ttcggcattg agatttcggg
     4321 cagtcatcgc tccgtaggac cggtagttgt tatgagttga ataatcgtca tattgatctg
     4381 cggccggatc ttcgttacag gcaccggcgg aaatggaatc accgtaaaac tcgatcgcaa
     4441 gcggcagagg ctcgggcttg gaacccattt cagttccgcg ttcaaggatc aacccacgga
     4501 atacggcgtt gctgaacatt gcctcggtcc gcttgaagat tatcacctca tgccatcctt
     4561 ccggcaatat ttcatccagt agataatcgt ggctgccgcc ctctaccagc tgcagcagga
     4621 taacccgtcc atcgatgata acattataaa aattttgccc ccaggcatcg gagaaacgaa
     4681 gacccaatct cctgcctgaa aaactgaacc tgagttcagt cccctgccag ataaaccggg
     4741 gcgcaccggg gtccttgcga tcaatccttc cgatgtaata taaaccggga tgctccgggg
     4801 tgataatctg ccggttcgtt tcttgagcgg catgaaccgt aaaggccggg aacttctcat
     4861 agcgccggtt tgacgaatca atggcagagt tctcttccat caggtcaccc cttgaaccag
     4921 gatacggcaa tgaatgttcc gataataaat ttactgtatc acaagaattt tccaattgtc
     4981 aatcgttcca atacaaacgc gccatatatg caattaatga ttcatatcgt tcgctgccgc
     5041 ttccatcaac aggaaaaacg cctgaccttc ggtagccgtt cccgggcgat cgaaattggg
     5101 cgctccgcat accccctgca ccagcccgaa ttgatccact ttttgataca cggcttcccg
     5161 gattctatcg gcctgggttt gataggaagg atttagccat ccgccttgaa ccccgcggta
     5221 cattgaatag ctcaacatct gcgcggcgtt tgtctccacg aaagtggcag gatcgtccaa
     5281 aacatcatgg aaaagtccgt cgctcctctg gaatttcaaa catccgtcga tggcctcttt
     5341 gacgaaaccc gccaacatat tcttctcttt ggtcatcgaa tcgggcaacg cccggataac
     5401 ccgggtcatc cccgccgcag cccatccgtt tccgacaccc caaaataact tccgctcaaa
     5461 ttgctgtaaa tcctcatccc aaatatgatg aaacagttgc ttttggggat cccacaaaac
     5521 tcgtcgatac cccaggatct gtttacaggc ttcgtcgggg tgaccggcaa ctgccagaaa
     5581 gggtggtacc atataaaaag cgtcaaccca gattcgattc tcaatttgat tatgatagat
     5641 aatcccgtcc cgtgtcttgg gtgcctggta taaaagatat tcaagcatcc gttgggcggc
     5701 tctgtggagg gcatcatccc cggtggcctt ggcggcaaaa agtaccggtt ctccattggc
     5761 ggcagggtcc gccacaggaa tccgatcgcc gtttaacccc agccggccgt ctttcagctg
     5821 attaactacc gcatcttttg ccagtaatat gactaaatcc gtctctccca actcgagcag
     5881 tgcttgggcg gccactccct gctcccaggc ccggcgttcc atggcgatca tcgtccggat
     5941 aaccttcccg attcgttctt cccgattcac ggcaatcccc tccggtttga tcaactttcc
     6001 cgcataaaat cggattacga tcagccttta cccctattct attccgtttt cacagatgtt
     6061 caggatgagt gtcctcatgt aacggttgat actgtagttc tcatccagat agatgagcat
     6121 cgcccgcgcc gcgtttaccg tatgctcgta tccgggcaga attctggcga gaaccggctc
     6181 cattccagga tacctctgct caaagcggcg ttctttggaa taaatatccc tacaaacctt
     6241 ttcatccgcc gcacaactca aggcgattaa ttcgataaat aagttgaatg cgttaccttg
     6301 aacgcctcta tatgcggcca gtttctcgcc ccggtagtaa cggcatactc ccacgtatat
     6361 attggtgatg atctcgccga gcagccattc gatggactcc ggtttccagt ccggcgcccc
     6421 ggtatgttga ggaatactaa gcttcgtgtc gaagccttcg tctttccaaa tgattctgcc
     6481 gggggcataa ggtatgtcgg ccaattccgg agtttcaaac acggcgaact cacaataaat
     6541 cccatcatcg aacatgatct tataaccatc cctggtgttc ctgaacttaa aaacaaccgg
     6601 tcggatcctc tccagccagt cagtattttc aatatagcgc tgtttatagc cgtcccggac
     6661 aatggcaaag aaatccagat cggaatagtt atccatccgg tgaacctctt gcccggcgga
     6721 tcccaaaccc attaacgcca aagcgttttc cgatgttttt aaggattcgg caatctcact
     6781 caatctttgt tgcagtcttt cctttcgctc catgaactcc ttcaccccct aattgatggc
     6841 caacgggtag taatccaata tcatctctat ttactttggc tcgtttatag tcgtaatcag
     6901 ctcactaaag ggtaattcct cgatggtccg gcagaaagca tgccattcga tgagcttatg
     6961 cacctttcgg gcatggtaca tattgcggag attggcgtaa ttgccggtcc aggtacgggt
     7021 atagttgaaa ctcatcggca gatcctgaat caactgtcgc cacaaacgat ttgcctcttc
     7081 gggccgctct ttttgcacag cctccttata aacggagatc aattcattta aatgttccag
     7141 gagtcccttt ttaaaggacg tcacttcatc ccagctgaaa tcgtcggcgg tcaggcttcg
     7201 ggaagtcagt ttatgcatgg tgctggtgct gttggcaacg gttcccacct tataggtgtc
     7261 catttctttc caccagtaat cgggggcggt gatatccatg ctgacgaaga tctgacggat
     7321 aaacttcccg tgatcgcttc ctgcggtaac cagtcgcaag gcgagttttc gatcattctc
     7381 gcccagagtc aataaggccc cgctttgttc attatagatc gaatccgaca gatgccagct
     7441 ggccatgggg ttcctcatgc cacgcaaggc gcctctcaaa ttgaaaacct cgacatgttc
     7501 tgttttaagc atcaaatggg ctccctccgg agaatattaa tatactaaga tcgtgccaaa
     7561 ttcgctttga ttcggttcga aattttaccg accacactct cgataaccgt accggagcaa
     7621 tctacgataa tatccgcgta cttttcatac aaaaccactc tttcgttata taaatcaatc
     7681 agcctttgcc ccttccccat ggcaataccc cggcttgaca tattcttgat gcggctctcg
     7741 atctcttcat acttgagttt aagatagatc agtttaccgt ttcttttcaa atggtttatg
     7801 gttgcttcac tgtatatagc gctcccgccg gtggcaatca cggaattttc ccgctctagt
     7861 tgcaggatta cggcttcttc catcgctaga aagcgctcca ctccatcttg atcaatgatt
     7921 gtctgcagta aacgtccgga cttttcctga attaacaaat ctgtgtccac aaaagacatt
     7981 cccaaggctt tggccagtaa aaccccgacg ctgctctttc ccgcaccggg cattccgatc
     8041 aataccacgt tggtttgcat ttttatcctc aaagttttta tcgttaatgc ctaatcatcg
     8101 gtctcgctga tatactccag cggtacccat ccaacctctc cgtttgtctg gttggtaccc
     8161 caaatccagc cgttgacctc tttttcccca ttgatgatat cgttcttccc gattctcatc
     8221 tctgaagccg agtattcttc caggatgatc cctgcgtttc cctggatttg aatgatctgc
     8281 cgcggaaccc acccttcatt tccttgattg cggcatcgta tccagtccgg ccatgcctct
     8341 ttaccggaat attcccctac gacttcaacc cgatcattgg gctttaagct gattgggtcc
     8401 ggatagtcgc tttcccgatc ctgaatcacg atatacttcc tgtttttttc aaaatgaatc
     8461 atgggtcttc cgcaaccgtt ctttttaggt cgatcatcgc ttcacctcaa attggcaggt
     8521 tacgaattca ccgcggtaat aagcatcact acagtcttca tcttttacgc acctcaaaat
     8581 gttatcaaag gaaaaattgt gtagagccga tcgagatcat aaaccgccga ccttcttgat
     8641 cactttttta accctgactt gctgggcggg ggttaaatcc tgcatgcgtt gttcgagaac
     8701 ttggataaag tgtaccttat tgccggtgtt tgcgatgatc atgcttcgct cggaatgttg
     8761 cgccacttcc cggggcttgc acgatgccag atgatcgagt aagaacggca agatgaccgt
     8821 ttcatattcg ttctttaccg agccgacctt ggccaaaaca gaaacgccgt tatctaccgt
     8881 tattaccgaa ccttgtttta tagccttaaa tatcaaatca acgttctcat agattttata
     8941 cgctctaata ggggcaatgg ttgataaggc ggtcatcgcc ccccagatca gccggttatt
     9001 cttgctcgtt aatagcctaa taaaagcctc aacatcatcc gcgatcagct ccggttgaac
     9061 ctcaccgatt tcataaagga ccttgatgct atcgttttga atcgcagcac taccactaaa
     9121 aagcaattga accagttcat gaatactatt ccgatcccct gtccggcata actccctcgc
     9181 caattcctga ttgggaactt catcatttct tcccagggag ctggctaatt tatccatgac
     9241 cgataccggc atgttgaccc tccttttggc atagccgtaa actatagaac ttacttttta
     9301 gccttggcat atgtacccga ctttttgacg gacataaaca cgtaataacc gtctatctcc
     9361 caaatccgga ctccaccgaa gattccggtc aatttattct gataccactc tttccgcttg
     9421 gtcaccataa ccatccgccc gcccacggct agccgattga agcctttttc aataaacagc
     9481 ttgggcactg aaaaatccac gtgatacgga gggtttgata aaatcaggct gaagtctttt
     9541 tcgtccaaat taccgaaacc gtcactttga agaatccgaa tccccggaac cccgttcaac
     9601 tcggcatttc gctttgcaag tccgacggca ccttcatcga tgtcaagcat ataaaccttt
     9661 tccgtaccga tgagcttggc tgcgaggatg ccgaccactc cgtagccaca tcccaaatcg
     9721 aggactttat cggtctcctg aaactcaatc acggacaaca tggcgagtgt accacgatca
     9781 atgctctttg gggaaaaaac cgtttcatgg gtttcaaaga cgaagtcaat atccttgatt
     9841 cttgtcctaa tcatttcgat tcgctgtata ttttttctcg gccgcttcga cagtctggcg
     9901 gatcagactg acaatcgtca tcggtcccac accgcccggc accggagtat aggccgaaca
     9961 attgggaacg gcatgttcga ggtcaatatc cccgatattg ccgggattgt aaccggcatc
    10021 cactaaaacc gcaccttttt tcacccaatc cccttgaata aacttgggct taccgactgc
    10081 ggcgacaaga atatcggctc tccgggtt
//
LOCUS       DASVMB010000018         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_296783,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000018 DASVMB010000000
VERSION     DASVMB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296783"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VF531_00585"
     CDS             complement(<1..848)
                     /locus_tag="VF531_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7712487.1"
                     /translation="MDAVRQQKIKQLNRHRWVVWLPLAFAFLAVYFHRTATGVVEDNL
                     MREFSIARASEIGGLASIYFYTYAIMQIPAGVLADFYGPRRTILLALLIAGLGAVSFG
                     MAGNVTVLYIGRSLSSLGVSLIYVNIAKIHAEWFRSREFTSITGLLVVAGSIGLLLAS
                     TPLALMVDLFGWRLSFMVIAGYTLIAAIFCWLLVRDRPADIGLPSITEVEAMEGVMQT
                     AAVPVKRYSVLESLKIVVGNPYTWWPFLASVMVYGVYMAFMGLWGVPYFMQIYGMSRV
                     DASNHI"
     gene            1162..2022
                     /locus_tag="VF531_00590"
     CDS             1162..2022
                     /locus_tag="VF531_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712488.1"
                     /translation="MKRFLLGFLVVFALILSGCGGGPGGGGLDDTSKNINSKLPVSSD
                     AETVSSTAVKKAEDKANPAVSHAPSLAFLPVVGLINKSGTGVDVRWSQHSDGWYYAEN
                     APAGVVYKYRETETGFQFHGERNTTEAISPPATGETSPGQSGALYTEGTLQFKGNFDL
                     TVTVNADNTWSGTQTTSYTDLSKGITYLDSTKTEVRGGWDDTFTGNATLTITNGNMND
                     GTGNFEYVGSMSYTDNIDETVEPGHIDLSIPTTEIRGTFTITRNGSVRTLSGTFTYDG
                     NTIPVNKTLD"
     gene            complement(2227..>2559)
                     /locus_tag="VF531_00595"
     CDS             complement(2227..>2559)
                     /locus_tag="VF531_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7712489.1"
                     /translation="AAAGKVLTDKNLKGKVHLTGLGLPSEMADYIENGICGWMYLWNP
                     IDVGYLTGYAAYALVNGSITGKAGESFKAGRLGVKKIIKVSDGTEIMLGDPFKFDKSN
                     IAEWKKVY"
BASE COUNT          717 a          625 c          573 g          644 t
ORIGIN      
        1 atgatgtgat tggaggcatc caccctcgac atcccgtata tttgcatgaa atagggaacg
       61 ccccagagac ccataaatgc catatatact ccgtagacca tcaccgatgc caagaaaggc
      121 caccaagtat agggattgcc cactacgatt ttcaggctct ccagaacgct gtaacgtttg
      181 accgggaccg ccgcggtctg cattacccct tccatcgctt ctacctctgt gatagaaggc
      241 aatccgatat ccgccggccg gtcccgaacc aataaccagc aaaaaatggc agcgattagc
      301 gtataacctg caatgaccat aaacgaaaga cgccaaccga ataaatcaac catcaaggcg
      361 agcggtgtgg atgcgagtaa cagtccgata cttcccgcca cgaccagtaa tccggtaatg
      421 ctggtgaact cgcggctccg gaaccattcc gcatggattt ttgcaatgtt tacgtagatc
      481 aagctcactc ccagactgga gagggacctg ccgatataga gcaccgttac attcccagcc
      541 atgccaaagg agacggcccc aaggccggca atgagtaagg ccaagaggat ggtacgcctg
      601 ggaccataaa agtcggccaa aacacccgcc gggatctgca tgatggcata cgtataaaaa
      661 taaatcgagg ccagtccgcc gatttccgat gcccgggcaa tggaaaactc ccgcatcaag
      721 ttgtcttcca ccactccggt ggcggtgcgg tgaaagtaaa cagcgagaaa ggcgaaggcg
      781 agcggaagcc agactaccca tctgtgccgg ttcagttgct taatcttctg ttgccgtacg
      841 gcgtccatct tagatcctct cccccgggaa aattaattcc agtgaaactt atgttttgat
      901 ggagccattc tccatttacc ggtattatcc tctctaaaaa aattgcccga cgacccatat
      961 tatcatatcc gccggaattt ttacggtgat tgccgtgttt tccggtttac atcccgaaac
     1021 gccaaaaaga acatcttttt aataattgac taattcattt tagggaaaat aatattagaa
     1081 taaatttgaa aatattgtta accggtgcta taatattatt acagtttttt atagtaattt
     1141 aaggaaatcg gaggcaatat catgaaaaga tttcttctcg gttttttggt tgtttttgcc
     1201 ttaattctat ctggctgcgg cggcggtccc ggcggcggag gattggatga cacttccaag
     1261 aatattaact ccaagctccc ggtctcttcc gatgcggaaa cggtttcttc aacagccgtg
     1321 aaaaaggcag aagacaaagc aaatcctgcc gtttcacatg ccccgagttt agcctttctg
     1381 ccggtagttg gtcttatcaa caaaagcggg accggggtgg atgtgagatg gagccagcat
     1441 tccgacggat ggtactatgc ggagaatgcc ccggccggag tggtttacaa atatagagag
     1501 accgaaacgg gattccaatt tcacggcgaa agaaatacca cggaagcaat cagccctcct
     1561 gccactggtg aaacttcccc tggtcaatct ggagctctct ataccgaagg aacactgcaa
     1621 ttcaaaggga atttcgactt gacggtaacc gtaaacgccg ataatacctg gtcaggtacg
     1681 caaacaacga gttatactga cctcagtaaa ggaataacgt atttagatag taccaaaaca
     1741 gaagtacgcg gtggctggga tgatactttt acaggcaatg caactcttac cataaccaat
     1801 gggaacatga acgacgggac tggtaatttt gaatatgtag gatcgatgtc atatacggat
     1861 aatatagatg aaacagttga accaggtcat attgatctct caatacctac tacagaaatt
     1921 cgtggcactt ttactatcac caggaacggc agcgtgcgta cgctttccgg aacatttacg
     1981 tatgatggta atacaatacc tgtaaataaa actcttgatt aaaaaagttg tacttactta
     2041 tcgtttggga taagtcaatt accttcaaga tttcacaaaa aagcatgggt cttcgatgga
     2101 taaaaaaggg ggtggaagtc ttttggattt ccaaacccct ttttattggt cccaatgaat
     2161 atatctttac ttccggcaaa aggcttcacg cgtcagccga atcggtcggg tcttcatttg
     2221 aaatccctag tagactttct tccactcggc gatgttcgat ttatcgaatt taaagggatc
     2281 ccccagcatg atctcggtgc cgtcgctcac tttgatgatc tttttcacac ccagtcttcc
     2341 agccttgaac gattccccgg ctttgccggt gatgctccca ttgaccaggg catatgccgc
     2401 atacccggta agatatccaa catcgatggg gttccacaga tacatccatc cgcaaatgcc
     2461 gttttcgata taatccgcca tttcactggg cagtcccaaa ccggttaagt ggactttacc
     2521 tttcagattt ttgtcggtaa gcactttccc ggctgcggc
//
LOCUS       DASVMB010000019         7144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_76707,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000019 DASVMB010000000
VERSION     DASVMB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76707"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VF531_00600"
     CDS             <1..829
                     /locus_tag="VF531_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7712490.1"
                     /translation="LSNNVEDIFNAAKSGDVTAVKAYLETGFDLNNKLDEYDNTVLII
                     AAQYGRAEIVKFLIENQVEIDAKDSDGMTALMLAAKGGHTDTVRLLLEAGADITVTDH
                     KGWTALMGAAIYGEPEVVKLLLEAKSDVNIRNTSNETALMMAALRGNTAALQLLIEAN
                     ADVNLKRNDGRTALMEAAFYGYGASGRSESEEEKYHVECVRRLIAAGADANAKTDTGL
                     TTLMITAYGNHLESVELLIAAGADLNSKNDKGATALTLAEGQKHSNVVTLLKKAGAK"
     gene            966..1775
                     /locus_tag="VF531_00605"
     CDS             966..1775
                     /locus_tag="VF531_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712491.1"
                     /translation="MDSVFKTSNTIVTEKDGVIIIRQRNRTIILIAAIELVIGVLSYM
                     ASQMRLSGDGGIERFCFWAAAIILPLAGLMFILGLLQGINAKVIFDCNSGKMRKGHHI
                     YEFSQIEKLSLESMPFADSEIFFVTASINRKPIKLISETSKATLEEVAEFLNRKLAIR
                     EAAATAVKGDNPSTASWPGRHFIGILLIALGVIWTGTGFFFLQNLIFTGWGNEHGPLI
                     WPLGIWIAGLGLGDLIGLPVGRVLKGGSRSSRIAMLIIILYFGSYFLICWR"
     gene            1822..2253
                     /locus_tag="VF531_00610"
     CDS             1822..2253
                     /locus_tag="VF531_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712492.1"
                     /translation="MGSLSDGCKKLLEQLKDAISGEDFIDAAEEPLERLSEMPDSFET
                     VEPILKLMEENPEVDYGMPGPLVHFLEQFFQNGYEEKLVESLERRPTKHTVWMLNRII
                     NGVEGDRKTYYLGILEKVIAFPGLEQDVKEQAQHFYSLRAN"
     gene            complement(2398..3126)
                     /locus_tag="VF531_00615"
     CDS             complement(2398..3126)
                     /locus_tag="VF531_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7712493.1"
                     /translation="MKRKNIWLGLTIIFLLGSVIVFGKEGKVDYSKNGVCIVPASIRA
                     EANQNGKWLSSLSVGETVTLLSAPRKDPKDPKIEYVKVKLSDGTEGYVSTWCLIQGAY
                     VAVVHRTARIYKRPDLLSDSNTSFEMLNIVAIDEEKGTWVRATGENRTKSGWIERVAT
                     RKEKEDIVTAVLLNKALKGKEKTMTRAEMEKIVAGLPYPNNYFAVKILQKYPAAGTNP
                     EPTSADQTDASGDTDSETTSDTTN"
     gene            3460..5379
                     /locus_tag="VF531_00620"
     CDS             3460..5379
                     /locus_tag="VF531_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HEX7712494.1"
                     /translation="MKRKKTLKNRLNYWFDNILSKGSAALISWLAVLSLILILGVSAV
                     ANYTRAALDQNGKPLSFLQVVWMSLMRAMDSGTIAGDSGGFLFMALMLLVTLGGIFIV
                     STLIGILNTGIEQKLDNLRKGKSFVVEHGHTIILGWSSHVTAIISELVVANSNKRHSC
                     IVILADRDKVEMEDEIRSKLGSTGRTRIVCRSGNPMRLNDLAMVNPNDSKTILVLAPD
                     EGDPDIQVIKTILAITNNPDRRRAPYHIVAEVHDPKNLQVAKMVGRDEVELLLAGDLI
                     ARIAVQTCCQSGLSIVYTELLDFGGDEIYFNHEKKLLGKTFGEAMLAYEDSAIIGIRF
                     RDGRVQLKPPLDTQIQTGDQLIAISEDDDTIRLSNRKDYKINEAAIQKSEREERQPER
                     TLVLGWNPWGMTIVNELDNYMPPGSAVTVVANLPETWEEVSRAINDRNQDWKNQKVKF
                     LPGDTVDRRTLDDLAINTYQHIIVLSYSGTLDAEAADAQTLVTLLHLRDIAEKSHAVF
                     SITSEMLDAKNRELAEIAHADDFIVSDRLISLMMSQVAENKQLMPVFADLFDPEGAEL
                     YLKPAVNYVKPGVPVNFYTVTEAARRQNEIAIGYRLDHQAKDATRAYGVRVNVNKSEM
                     VTFTEKDKVIVLAED"
     gene            complement(5560..6432)
                     /locus_tag="VF531_00625"
     CDS             complement(5560..6432)
                     /locus_tag="VF531_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX7712495.1"
                     /translation="MSKRMIAVVTLFLLAILAIHPVALAAVQIGVGAQLKNEQFDNTV
                     AMNWWLTDNWALRGDYSWNTEDLGIAALYEINPGSNAALYIGVAKNDLFTNETPDPKV
                     SRTGAQLIAGMEWNLSRIKPGLSLMTEAQIDPGDLLNQSVVDNEPVSKFSITINYRFL
                     SPNIAANTYDPNTARLLAKLIMLEANDEPFEGQVAVAAVVLNRTHDSRFPDSVAKVIY
                     QAGQFSTARKIAGIVPDESALNAANTALRGNDPSHGALYFYNPATASKSARHYIKKAR
                     FQVTARIGHHVFMK"
     gene            6635..>7144
                     /locus_tag="VF531_00630"
     CDS             6635..>7144
                     /locus_tag="VF531_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HEX7712496.1"
                     /translation="MRSRGSLTILFMMALVLCLGLNGLSAAATGNSIPEGSIRIHYIR
                     ADQNYEGWGLHLWGEGYNGPAVTWSESAPLSGIDDYSCYWDVAYKGSGQMGLIIHQGD
                     KKDPGPDQFFKDPGCREVWIVSGSNDIFPEFDEALAKAGLKKLDIPALAASNVRVHYR
                     RADGNYKGWA"
BASE COUNT         1905 a         1473 c         1846 g         1920 t
ORIGIN      
        1 tcttagtaat aatgttgagg atatctttaa tgcggccaaa tctggggatg taaccgctgt
       61 gaaagcttat cttgaaaccg gttttgatct caacaacaag ctggatgagt atgataatac
      121 ggtgttaatt attgcggctc agtatggtcg cgctgagatc gtgaagtttt taattgagaa
      181 tcaggttgaa atcgatgcca aagatagcga tggcatgacc gctttaatgt tggccgcaaa
      241 gggaggccat accgataccg tcagattatt gctggaagcc ggtgcggata tcactgtaac
      301 agatcataaa ggctggacag cactaatggg tgcggctata tacggggaac ctgaagtggt
      361 gaagctgtta ctggaggcca aatcggatgt caatattaga aatacaagta atgagactgc
      421 gctaatgatg gcggcgctaa ggggaaatac tgcggccttg caacttctga ttgaagcgaa
      481 tgccgatgtc aatctgaaga gaaatgacgg aaggacagct ttaatggagg cggcttttta
      541 tggctatggt gcttccggca gatccgagtc tgaggaagaa aaatatcatg ttgaatgtgt
      601 acgacggttg attgctgcgg gggctgatgc gaacgccaag acggataccg gtttgacaac
      661 cttaatgata actgcttacg gcaatcacct tgagtcggtt gaattgttga ttgcggcagg
      721 ggcagatctt aactcaaaga atgataaagg tgccactgca ttgacgttgg ctgaaggaca
      781 aaaacattcc aatgttgtaa cactattgaa aaaggcgggg gctaaatgaa tttgaaatcg
      841 gagcaaagac aatctcattg acaataacaa gaaaaatagg atcggtattt ggagataacc
      901 ggatattata caagtgggag aaatatgaat ggacattttc caagatgaaa ggaaggagat
      961 catttatgga ctcggttttt aaaacttcta atacaatcgt gaccgaaaaa gacggggtta
     1021 tcattatcag acaacggaac cggacgatta tcctcattgc cgcgattgag ctggtaatag
     1081 gggtactgtc atacatggca tcgcagatgc gtctttcggg agacggggga attgaacggt
     1141 tttgtttttg ggcggcggct atcatcctac ctttggcagg actgatgttc attttgggat
     1201 tgcttcaggg cataaacgcg aaggttattt tcgactgtaa ctccggaaag atgagaaagg
     1261 gccatcatat atatgaattt tctcaaattg agaagctgag tctggaaagc atgccttttg
     1321 ccgatagtga aatattcttc gtaacggcat ctataaatcg taaacccata aaattgattt
     1381 ccgaaacatc aaaagccacc ctcgaagaag tagcggagtt tttaaaccgg aaactagcga
     1441 tccgagaagc tgcagctacg gcagtaaagg gtgataatcc atcaacagca tcttggccag
     1501 ggcgacattt tatcgggatc ctgctgattg ccttgggtgt catttggacc gggaccggtt
     1561 tctttttcct tcaaaacctc atctttaccg gatgggggaa tgagcacggt cccttgattt
     1621 ggccgttggg tatttggatt gccggactcg gccttggaga tttgattgga cttcctgtag
     1681 gccgagtttt gaaaggtggt tcaagaagtt cgagaattgc aatgctgatc atcatattat
     1741 atttcggttc atatttcctt atttgctggc gctgattggg aaagatggaa aactgaagca
     1801 gatctgattg gaggcagaaa agtgggatca ttgagcgacg gttgtaaaaa gttattggag
     1861 caattaaaag atgctatctc cggtgaggat tttattgatg cggcggaaga accacttgaa
     1921 agattaagtg aaatgccaga ttcctttgag acggtggagc cgattttaaa gctaatggaa
     1981 gaaaacccgg aggttgatta tggaatgccc gggccgctgg ttcattttct cgaacaattt
     2041 tttcaaaacg gttatgaaga gaagctggtt gagtccctgg aaaggcggcc taccaaacac
     2101 actgtctgga tgctcaatcg gattatcaac ggggttgaag gcgaccggaa aacatactac
     2161 cttggaattt tagaaaaagt aattgcattc cccggcctgg aacaggatgt gaaggaacag
     2221 gctcaacatt tttactcatt gagagcaaat taacccaccc tgttgaacct agaaacggaa
     2281 ggcaccccca atgaaagaga agatgactgt cagcatttga ttgggttcat acagtgttgc
     2341 tccgggggga aagagactgc catatcaatg gcagtctcta atgttatgag agggttatta
     2401 attcgtcgta tcggaggtgg tttcagaatc cgtatctccg gaagcgtcag tttgatcggc
     2461 ggaagtgggt tccgggttcg tacccgcagc cggatatttt tgcaggattt ttacggcaaa
     2521 gtagttattc ggatacggca gaccggctac gatcttctcc atctccgcgc gggtcatggt
     2581 tttttcttta cccttcaacg ctttatttaa gagtacggcg gtcacaatgt cttccttttc
     2641 tttgcgggtc gccacccgct cgatccagcc gcttttggtg cgattttcac ctgtggcccg
     2701 gacccaagtg cctttttctt catcaatcgc cacaatattc aacatttcaa aactggtgtt
     2761 ggaatcggaa agcaaatcgg ggcgtttata aattctggcc gtccggtgta caacggcaac
     2821 gtaggctcct tgaatcagac accaggtact tacatatcct tcggtgccgt cggagagctt
     2881 taccttaacg tattcaattt tgggatcttt cgggtctttc cggggggcgc tcagaagggt
     2941 cacggtttca cccaccgata aagacgagag ccatttgccg ttttggttgg cttcggcgcg
     3001 aatggacgcg gggacaatgc aaacaccgtt tttggagtag tctacctttc cctcttttcc
     3061 aaagacgata accgatccga gtaaaaaaat gattgtcaac ccgagccaaa tatttttgcg
     3121 tttcaaaata atcatccttt cattaatttg atacctctct cataaaataa taatattttc
     3181 ccatgggaaa atctagggtt taatcaaaac ttcattgaaa ttcgtgcaag tcattcgata
     3241 acttcaaaac tgaaactttg gaaaggaaaa gaggaaaaac caggaagggt acggcgatga
     3301 gacagaagcc tgttagattt acttgtgctt tgaattgctt atcattgtat tttggcagga
     3361 aaattgtaaa aaaagccgaa actaattata tatttcacat aaaattgttc caacgattat
     3421 gatttaaatt atcatttgaa tggaaaggaa cgtatcgtta tgaagcggaa aaaaacttta
     3481 aaaaatcgtc tgaattactg gtttgacaat attctctcca aaggttcggc agctttaatt
     3541 agttggctgg cggtgttgtc gttgatcctg attttgggag tctccgcggt ggccaactat
     3601 acccgggcgg ccttggatca aaacgggaaa ccattaagtt ttttgcaggt cgtgtggatg
     3661 agtctgatgc gggcgatgga ttccggaaca atcgccggag atagcggcgg atttctcttc
     3721 atggctttga tgcttctggt tacgctaggt ggtattttta tcgtcagtac cttgattggt
     3781 atcttaaata ccggcattga gcaaaaattg gacaaccttc gcaaagggaa atctttcgtt
     3841 gtcgaacatg gacataccat catcctcggt tggtcatcgc atgtgaccgc gatcatttcg
     3901 gaattggtgg ttgccaacag caacaaacgt cattcctgta tcgtgatcct ggcggatcgg
     3961 gacaaggtgg agatggaaga cgagatccgc tccaaattag gatcaaccgg aagaaccagg
     4021 attgtctgcc ggagcggtaa tccgatgcgc ttaaacgatt tggcaatggt caatcccaac
     4081 gattccaaaa cgattctggt attagccccg gatgagggtg atccggatat acaggttatc
     4141 aagaccatcc tggccattac gaataatccg gaccgccgta gggcgccata tcatattgtc
     4201 gccgaggtcc atgacccaaa gaatctgcaa gtcgccaaaa tggtggggcg tgacgaggtt
     4261 gaactattat tggcgggtga tctgattgcc cggattgctg ttcaaacctg ttgtcaatcg
     4321 ggtctttcga ttgtctatac cgagttgttg gatttcggcg gggacgagat ctattttaac
     4381 catgagaaga aattgctcgg caaaactttt ggtgaagcga tgctggcata tgaggattcg
     4441 gcgatcatcg gaatccgctt cagggacggc cgggttcaac tgaaaccgcc gcttgacact
     4501 cagattcaaa cgggagacca gcttattgcc atttctgagg atgatgacac catccgtctt
     4561 tccaatcgta aggattacaa gattaacgaa gccgctattc aaaaaagtga gagggaagaa
     4621 cgccaaccgg aacggacttt ggttttgggc tggaatccct ggggtatgac gatcgtcaac
     4681 gagctggaca actatatgcc gccggggtcc gcggtaacag tggtcgccaa cctgccggaa
     4741 acctgggagg aagtttcccg ggcaatcaat gatcgtaacc aggattggaa aaatcaaaag
     4801 gttaagtttc tccccgggga tacggtggat cgccggactc tcgatgactt ggcaatcaac
     4861 acctaccagc acatcatcgt cctcagttat tccggtacat tggatgcgga agcggcggat
     4921 gcccaaacct tggtaacact gcttcatcta cgggatatcg ccgagaagtc ccatgccgtc
     4981 ttttcgatca ccagtgagat gctggatgcc aaaaatcgtg aattggcgga gattgctcat
     5041 gccgatgatt tcattgtcag cgatcgattg atcagtctga tgatgtcgca ggtggccgag
     5101 aataagcaat tgatgccggt ttttgccgat cttttcgatc ctgagggtgc cgaactctat
     5161 ctcaaaccgg ctgtaaacta tgtgaagccc ggagttccgg tcaattttta tacggtgacc
     5221 gaagcggccc gccgtcagaa tgagattgcc atcggttatc gcctggatca tcaggccaaa
     5281 gatgcgacac gggcttatgg agtgagagtt aacgtgaata agtcggagat ggtgaccttt
     5341 accgaaaaag ataaggtgat tgtactggct gaggattgag tattcatgcc gtagtgaaaa
     5401 ttctcatctt ttttacttag aaaacttgat tgttcaataa agcgatagga gcgttgcgtg
     5461 acaggttata ttgacctgcc aagttacgct cctttttttg ttttatcata tttaatattc
     5521 aatcatcaaa aaatccgtag tctttgcttt ccccaatcgt tatttcatga aaacatgatg
     5581 tccaatccgg gcggtcacct gaaaacgggc ttttttaata taatgcctgg cgcttttgga
     5641 agcggtggcc gggttgtaaa agtatagggc gccgtgagac ggatcgttgc cccttaacgc
     5701 ggtatttgcg gcatttaaag ccgattcatc cggtacaatc ccggcgattt ttcgggcagt
     5761 actaaactgt cccgcttgat aaataacctt cgccaccgaa tccgggaagc ggctatcgtg
     5821 ggtccggtta aggaccaccg cggccactgc cacctgccct tcgaacggtt catcattcgc
     5881 ttccagcata atcaacttcg cgagcagacg ggcggtattt ggatcatagg tatttgctgc
     5941 tatatttggc gaaagaaacc ggtaatttat ggtgatgctg aacttggaaa ccggttcgtt
     6001 gtcgacaacg gattggttga ggagatcacc gggatcgatt tgcgcctcgg tcatcaagga
     6061 cagacccggt tttatccggg ataaattcca ttccatcccg gcgatcaatt gtgcgccggt
     6121 gcgggacact tttggatcag gcgtttcatt ggtaaataga tcatttttcg ccacaccgat
     6181 gtaaagagcc gcatttgacc cggggttgat ttcgtaaagg gcggctatcc ccagatcctc
     6241 cgtattccat gaataatctc cacgtaaggc ccagttgtca gttagccacc aattcattgc
     6301 aaccgtatta tcaaattgtt catttttcag ttgtgcgccg accccgattt gcactgcggc
     6361 gagagcaaca ggatggatcg caagaatcgc taaaagaaac agggtaacca cagctatcat
     6421 tcgtttcgac aattcatcca cctcaaatgg atgcttcgct tgttttttgg ccggttcctg
     6481 cctgaatata atgtaaaaca tgggaagtac cggtttttaa tatgttcagt ctttacccat
     6541 ttttttgaaa gagaagtagg attaatggta gaaaaatgga atataattat agtgcaaacg
     6601 gttgcacaaa tttcggacga aaaggaggag ttcgatgagg agtagaggtt cattgactat
     6661 tttgtttatg atggcgctgg tgctttgcct ggggctaaac ggtctcagtg ccgccgcaac
     6721 cgggaacagc attccggaag ggtccatccg gattcactac atccgggcag accaaaacta
     6781 cgagggttgg ggattgcatc tctggggcga aggctataat gggccggcgg ttacctggtc
     6841 cgagtcggcg cccctcagcg gtattgacga ttatagctgc tattgggatg tggcttataa
     6901 aggatcgggc cagatgggct tgattattca ccagggtgat aaaaaagatc cgggtcccga
     6961 ccagtttttt aaagatcccg gttgccgtga agtttggata gtctccggaa gtaacgatat
     7021 ttttcctgag tttgacgagg cgttggcgaa agcgggcctt aagaaactgg atattccggc
     7081 cctcgctgca agcaacgtgc gggtgcatta tcgccgcgcg gatggtaact ataaaggctg
     7141 ggcc
//
LOCUS       DASVMB010000020         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_517844,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000020 DASVMB010000000
VERSION     DASVMB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517844"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..804)
                     /locus_tag="VF531_00635"
     CDS             complement(259..804)
                     /locus_tag="VF531_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEX7712497.1"
                     /translation="MTRKTVTDWAKKNFLHWFIENYLAGTEDVQKILIQIAETDSLLA
                     KIHIISDGSYLRPLLVISTEDTGMPPFLLKTFDTTVTETGLIKDRLQLLGESPIYLTF
                     YFPDRATCEPFQAVAEENPFSLDSASARQLLIDFELSLWSETFKQEIERSELMTQIDE
                     ALDEKNERKFLSLVKKLKNLK"
     gene            1040..2152
                     /locus_tag="VF531_00640"
     CDS             1040..2152
                     /locus_tag="VF531_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7712498.1"
                     /translation="MEDPRIEKMAQGLLHYSVRLQPGEKILIEVIDAGMDLARALLKE
                     VYRMKGVPYTLVRNKQLDREFMMGANEDQLRRLAEYELVQMKEMDAYLGIRGSENSNE
                     LADVPGEQQQKYMKYFWRPVADERINHTKWCIMRYPTPSMAQAANMSTAGFEDFYFDV
                     CTLDYSKMAQAMEPLKELIERTDQVRLTGRDTDLTFSIKGLPAIPCSGQMNIPDGEIF
                     TAPVKDSVNGVITYNTPAVYQGVTYEQVQLHFSNGKIVQISGSDPERLTQIFNTDEGA
                     RYVGEFSFGVNPYILKPMKDTLFDEKISGSIHFTPGSSYQECDNGNKSAVHWDLVFIQ
                     RPEYGGGEIYFDGRLIRKDGRFVVPELEQLNPENLV"
     gene            2264..2806
                     /locus_tag="VF531_00645"
     CDS             2264..2806
                     /locus_tag="VF531_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX7712499.1"
                     /translation="MKEIYSFRAQVHDKPSGPITRWIMAIQTKEFRFTKAVYFRNLLD
                     NYLQKSWWAVFFLGFVVIYAAYRGERQTLWICMGLALAYPIYILIRCFIHTNSKRNRL
                     FFAERYFVIDNQLITGHFHDGSTNRIQLSSIVRVIKKGGAYQLFISKRQFIYIPVSCF
                     KTPQDIERFDKLLKQRKPSQ"
     gene            complement(2900..4207)
                     /locus_tag="VF531_00650"
     CDS             complement(2900..4207)
                     /locus_tag="VF531_00650"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7712500.1"
                     /translation="MGSVVAKFGGTSLADATQFRKVQEIIRANPDRRYIIPSAPGKRN
                     RDDYKITDLLYLCHTHVEQAIPFDDVFSIISDRYLAIIAELGLGLDLRPVLQDIKTRI
                     AAGASTDYVASRGEFLNGLILAELVGYTFIEPAETIYFTKQGQLDLERTEKVLGERLV
                     QIPRAVIPGFYGVTPDGAIKTFSRGGSDITGSIVARAARADLYENWTDVSGFLMADPR
                     IVPNPRPMSRITYRELRELAYMGATVLHEEAIFPVRDAGIPINIKNTNQPDHPGTIIV
                     DRAEPGDTITGIAGKKDFTVIAIEKALMNTELGFGRKLLSILESNSVSFEHLPSGIDT
                     ISVVIADSQLDGKLDKILEEIRQQCRPDAIEVYPNMALIATVGRGMVYTPGIAGKLFT
                     ALAVEQINVRMIDQGSSELNIIVGVESNDFEKAVRAVYQAFVA"
     gene            complement(4736..4975)
                     /locus_tag="VF531_00655"
     CDS             complement(4736..4975)
                     /locus_tag="VF531_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEX7712501.1"
                     /translation="MLTCEVSLLPMETVKADEIINQSLTALKATGVKHEVGNMSTYLY
                     SEDPAQIWNGLKTIYETAEKAGTEFSMVINLSNNT"
     gene            5086..6123
                     /locus_tag="VF531_00660"
     CDS             5086..6123
                     /locus_tag="VF531_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7712502.1"
                     /translation="MRITPIEEIKPGMKLARAVYREEDGSVLLRPNIELKSNYIERIK
                     ELKYSHVYTLNPDESGEEIIDDPVDEELRFRARGLLKKTVGMLKSEEKVSLSRISVLV
                     AEMVEQVYRQTDIVYNMVDIRSRENYLYAHSVNVCVIALMIGASMSLNRMDMEKMGVG
                     ALLHDLGLLAGDTPSAQGKGSSHSGGPEAAKQHPRAGYELLKQKTELNFLAAHVALQH
                     HEREDGSGYPRGLTGKRIHRFAKIVAVADAFDTLTHRSFQKGLAPYQAIQDIKADQGK
                     YDEAVVDNFLKVMAPYPIGSVLLLDSGETVLVERVSRYECQVRVLAGGVQGTVYNLYQ
                     ASKIKVVQFLN"
     gene            6419..>6629
                     /locus_tag="VF531_00665"
     CDS             6419..>6629
                     /locus_tag="VF531_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712503.1"
                     /translation="MVKDQCDVLILSASYGGGHNQVARALTQAMMVQMPGIKVATVNF
                     TDLLIPFFTRLSRFGYMQSIRHFPAG"
BASE COUNT         1714 a         1472 c         1705 g         1738 t
ORIGIN      
        1 ccggccggaa acaggttaga ctatcccttg ctttgttaat tatatgcttt tccgagttta
       61 aatatcactt aaaacgtaaa aaccggttga acgttacttt ttaagaaaag ggtaaaaaaa
      121 gaccggccaa ggccggtcaa aaaagtgatt taagtggaag agctttgggg gaagataggg
      181 aacagggttc aacatcaacc aatcggtttt ttcgataaga acggcctgta agaggtttaa
      241 ggaaagatcc ggcaccaatt atttcaggtt ctttaatttt ttaaccagac ttagaaattt
      301 ccgttcgttt ttctcgtcga gggcttcatc aatctgagtc attaactcgg accgctcgat
      361 ctcctgctta aaggtttcgc tccacaagga gagttcgaaa tcgatcaata actgccgggc
      421 gctggcggag tcaagggaga atggattttc ttcggcaacg gcctgaaaag gctcacaagt
      481 tgcccgatct gggaaataaa aggtaagata aattggggat tcgcccaaca attgcaggcg
      541 atctttgatt aacccggtct cagtgactgt agtatcaaaa gtttttaaaa gaaatggagg
      601 cataccggta tcttctgtgg aaataactaa taaaggccgg agatacgatc catccgagat
      661 gatatgaatc ttggcaagca aactgtcggt ctcggcgatt tggatcagga tcttctggac
      721 atcttcggta ccggcgagat agttttcgat gaaccaatgt aaaaaattct ttttggccca
      781 gtcagtaaca gtttttctag tcataacgac ctcggttgag ttattttagt tacatccatc
      841 tcttttatca taatattcgg ttgatatcaa tctcatactg agaggtaatt aattacaaca
      901 atttgtttca aaggttttag gcggatccgg tatgttttgg atggctgtaa ccgtaaatct
      961 tattaagcgc acatcatccg gggcggtttt ccctccgggt gttgttccat gaaagaagat
     1021 cataaaagga gtggtcggtt tggaagatcc acgtattgaa aagatggctc aagggttgct
     1081 tcattattcg gtccggttgc aaccgggaga aaagattttg attgaggtaa ttgacgccgg
     1141 gatggacctg gcccgggcgt tattaaagga agtttaccgg atgaaggggg taccttatac
     1201 gctcgtccgg aataagcagt tggaccggga gtttatgatg ggtgcaaatg aggaccaact
     1261 gcgccgtctg gccgagtatg aactggtgca gatgaaagag atggatgcct acctggggat
     1321 tcgtggctcg gagaactcca atgaactggc ggatgttccc ggtgagcaac agcaaaagta
     1381 tatgaaatac ttttggcgtc cggtagccga cgagcggatc aatcatacga aatggtgcat
     1441 catgcgttat cccactccgt caatggccca agcggcgaat atgagtacgg cggggtttga
     1501 ggacttttat ttcgatgttt gcactttaga ctatagtaaa atggcacagg ccatggaacc
     1561 gctaaaagag ctgatagagc gcaccgacca ggtacggctg accggccgcg atacggacct
     1621 aaccttctcc attaaagggc tcccggcgat tccctgttcc gggcagatga atataccgga
     1681 cggagagatt tttaccgcgc cagtcaagga ttcggtcaac ggtgtaatca cttacaatac
     1741 cccggccgtc tatcagggag tcacttacga gcaggttcag ctccatttca gcaacgggaa
     1801 aatcgtccaa attagcggga gcgatcctga acgcctaact cagatcttca ataccgatga
     1861 aggggccaga tacgtcgggg agttttcttt cggggtcaac ccttacatct taaagccaat
     1921 gaaggatacg ctttttgatg agaagatcag cggcagtatt catttcaccc cgggcagttc
     1981 ttatcaggag tgcgataatg gtaataaatc cgcggttcac tgggatctgg tatttatcca
     2041 acgtcccgag tacggaggcg gagaaatcta tttcgacggc cggttaatcc gtaaagacgg
     2101 ccggtttgtc gtaccggagc tggagcagtt aaacccggaa aacctggtct aaccgcgtac
     2161 cggagtcatc tgttgcgacc ggtagccatc gtcggtaccg gttttccggc aagcggactt
     2221 ttcctcgcga aagccggttg gtaaggcttt cgcgaggatt cggatgaagg aaatctattc
     2281 attccgggct caggtgcacg ataaaccgtc cggcccgatt acgaggtgga ttatggcgat
     2341 tcagaccaaa gaatttcgtt ttaccaaggc ggtttacttc cggaacttgc tcgataatta
     2401 tttgcagaaa agttggtggg ccgtattttt tctggggttt gtagtcatct acgcggctta
     2461 ccggggtgaa aggcaaacgt tatggatctg tatggggtta gcgctcgctt atccgattta
     2521 tattttgatc cgttgtttta ttcataccaa ttccaaacgg aaccggttgt tctttgcgga
     2581 acgatatttc gtcatcgaca atcaattgat taccgggcat tttcacgacg ggagcacgaa
     2641 ccggattcaa ctttccagta tcgtccgggt cattaaaaag gggggtgctt atcaactctt
     2701 tatctccaaa aggcaattca tctatattcc ggtcagttgt ttcaagaccc ctcaagacat
     2761 cgaacgtttt gacaaattgc tgaaacaaag aaaaccatcg caatgatggt tttattgtcg
     2821 aggcagttct gtaattaccc ggaactggct gcaaatttga cagacctgtc gagataaagg
     2881 tttaagcggg atccggttct taagctacaa aagcctgata gacggcacgg acggcctttt
     2941 caaaatcatt actttcgacc ccgacgatga tattcagctc gcttgacccc tggtcgatca
     3001 ttcggacgtt aatctgctcc acggccaaag cggtaaagag tttcccggca atgcccggag
     3061 tgtacaccat gccccggccg actgtggcga tgagcgccat attgggatag acttctatgg
     3121 catcgggacg gcattgctgc cggatctcct caaggatctt gtcgagtttc ccgtccagct
     3181 gggaatcggc gataacaacg gagatggtat caatccccga cggcaggtgt tcaaaggaga
     3241 cgctgtttga ctccagtatt gagaggagtt tcctgccaaa gccaagctcc gtgttcatca
     3301 aggccttttc gatggcgatc acggtaaaat cttttttccc ggcaatgccg gtaatggtat
     3361 ctcccggttc ggcccgatcc acaataatgg ttccgggatg gtccggctgg ttggtatttt
     3421 tgatatttat ggggatacca gcgtcacgaa ccggaaagat ggcttcttcg tgaaggacag
     3481 tggctcccat ataagctagc tcccgtaact cccggtaggt aatccgggac atgggccggg
     3541 gatttgggac aatgcgggga tcggccataa ggaatccgga aacatcggtc cagttttcat
     3601 agagatccgc ccgtgccgcc cgggccacaa tggagccggt gatatcggaa ccgcccctgg
     3661 agaaggtttt tatggcacca tcgggggtga caccgtaaaa tccgggaatt accgcgcggg
     3721 ggatttgaac cagccgttca cccaaaacct tctcggtacg ttccaggtcc agttggcctt
     3781 gtttggtgaa gtagatcgtt tccgccggct ctataaaggt gtacccgacg agttcagcca
     3841 ggatcaaacc gtttaaaaat tcaccgcggc tggccacgta gtctgttgag gctcctgcgg
     3901 cgatccgagt tttaatatcc tgtaacacag gccttaaatc caaccccaat ccgagttcgg
     3961 caatgatagc caaataacgg tcggagatga tggaaaacac atcgtcaaac gggatggctt
     4021 gttccacatg ggtgtggcag agataaagca gatccgtgat cttgtaatca tcccggttcc
     4081 gttttcccgg ggccgatgga atgatgtagc gccgatcggg atttgcccga atgatctcct
     4141 gaaccttccg gaactgcgtc gcatcagcca atgaggtgcc accgaatttt gccacaactg
     4201 aacccaattg aacaaacccc cttaaccgtc gctagctatt taagaactcg gttatttttt
     4261 acagctttat tcaacataat aaggccaaaa cctggaaaag tcaagcgtaa tcaagatccc
     4321 gggccgtgaa tggcacgcaa cggatgtgaa tatataaaaa agggtactaa gtattttact
     4381 tagcacccca gtggtatatc aaagatagaa tcggacgatg aaactacttc ttcttcggcg
     4441 gctttttggt ttctttcttc ggcgattggt ccttggccat ctgactgaca cccccttttt
     4501 ataaactgaa tgatctttat ataatcattt ttacgccgga gccgtatttc ctgctgccac
     4561 cggagaaatt ttgcaaattt tgcataaaaa acagcttaat tacccgggga aaggtttcaa
     4621 aaacggaaaa cgatcgcaaa aagcaggatt tttcgtaatg caggccgaag tttacaggta
     4681 cttatgttcc cggagatcac tccactcgtc tccgggtgcg gaagagtatt ttatcttagg
     4741 tattgttgct gaggttaatg accattgaaa attcggtacc ggctttttcc gccgtttcat
     4801 agatggtttt caacccgttc cagatctgcg cgggatcctc actatacaaa taggtactca
     4861 tgtttccgac ttcatgtttg acaccggtcg ctttcaaagc cgtgagcgat tgattgataa
     4921 tctcatccgc cttcaccgtt tccatcggca atagggaaac ttcacaagtc aacaaaattc
     4981 tcaccccttt ccaatagata ggttctctaa tcggtagaat gagattccag gtagatttta
     5041 tcaacccagg ggaataatag attaatccgg ggggtacagg cacggatgcg cattactccg
     5101 atagaagaaa tcaagccagg catgaaatta gcccgagcgg tttatcgcga ggaggatggc
     5161 agcgttctgc tgcgtccaaa tattgaactg aaatcgaact acatcgaacg gattaaggaa
     5221 ttgaaataca gccatgtgta tacccttaat ccggatgaat ccggtgagga gatcatcgac
     5281 gatccggtgg atgaggagtt acgtttcagg gcccggggtc tcttaaaaaa gacagttggg
     5341 atgcttaagt ccgaagagaa agtcagccta agccgaatat cggtgcttgt ggccgaaatg
     5401 gttgaacaag tctatcgtca gacggatatt gtctataata tggtagatat tcgttcccgg
     5461 gagaattatc tttacgctca ttcggtgaat gtctgtgtga tcgctttgat gattggagcg
     5521 tccatgagtt taaaccggat ggacatggag aagatggggg tcggagctct tttacatgat
     5581 ctggggctgc tggccggcga taccccgtcc gcccaaggta aggggtcctc acattccggc
     5641 gggccggagg cggcaaaaca acatcccagg gccggctatg aattgctcaa acaaaagacc
     5701 gagttgaact ttcttgcggc ccacgttgcg ttgcagcacc atgagcggga agacggcagc
     5761 ggatatccac ggggactaac cggaaaacga atccaccgtt tcgcaaagat cgtcgcagtg
     5821 gcagatgcct ttgatacctt aacgcaccgg agcttccaga agggattggc tccatatcag
     5881 gcgattcagg atatcaaggc ggatcagggt aaatacgatg aagcggtagt cgacaatttc
     5941 ctgaaagtaa tggcgcccta tcccataggt agtgtgttac tgctggatag cggcgaaact
     6001 gtcctggtgg agagggtctc ccgttatgag tgtcaggtaa gggtgttggc aggcggggtt
     6061 caaggtacag tctataattt atatcaagcc tccaaaatta aagtggttca gtttctaaat
     6121 tgataacgat tttttggccg gtatctaggg ccccattttt ctatccccag tttttggata
     6181 ttctattgct tcgatggtaa actatggatc tgagtgcgtt attgggggtc atcttgggtg
     6241 aggtacacgg ttgaaacaaa aacccgacgt aattcgttaa atataggggt ttagttgggt
     6301 tttgcgcgcg cgagcgaatc cgaccttcca ataaactggc aggatttttt tatcctgctg
     6361 tcaaatttat tacatcactt gaaccgaagc gaatattccg gtcgaagaag gtatggatgt
     6421 ggttaaagat caatgtgatg tgttgatcct ctcggccagt tatggagggg ggcataatca
     6481 ggtggcccgg gctttaaccc aggccatgat ggtgcaaatg ccggggatta aagttgccac
     6541 cgtcaatttc actgatctgc tgattccgtt cttcacccgg ttgtcgcgtt ttggctacat
     6601 gcagagcatc cgccattttc cggcgggat
//
LOCUS       DASVMB010000021         5770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_517861,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000021 DASVMB010000000
VERSION     DASVMB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517861"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF531_00670"
     CDS             <1..208
                     /locus_tag="VF531_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7712504.1"
                     /translation="PQEYLIEYRIAKACRLMAEKGLTIGDIARSVGYQDPLLFSKTFK
                     QIKGESPRVYRNRMDGQADAKNGY"
     gene            423..1493
                     /locus_tag="VF531_00675"
     CDS             423..1493
                     /locus_tag="VF531_00675"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7712505.1"
                     /translation="MFNIAFFQQFSLGERFLWFVGLSVAGWILSKLLVLVWEKLILPL
                     ASKTQSSLDDHLAKNLHKPVIRLLILGSIYLAAKFTLLTAEQVKIYVAAAENLIYLIL
                     ILFVASLLNNILKSIIDWYLQDMASKTESTLDDTIFPLFRKAGAVIIYFIASTVILDK
                     FNVNLTGFLATAGVASLALAFAAQETLSNVIAGISILLDHSYHIGDRIELKDGLVGDV
                     SEIGLRSTKILSLDQRLIIVPNKDIAGSRLINWCQPNAATNIKLKIGVAMDEDLEKVK
                     RVVTEVCNQEASLSRKAPAAVVCTGFGPYFIELLVVATVDDCRTTGTATDHLVVKIQE
                     AFQREKIRLPYPLQQIQMQKVG"
     gene            complement(1734..2861)
                     /locus_tag="VF531_00680"
     CDS             complement(1734..2861)
                     /locus_tag="VF531_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7712506.1"
                     /translation="MSEPKDYIERMDNSLNQVFTTALKISFQNPSLALFILRSTLRLH
                     KAARSRRSWERRGIHVPPYLIASITNGCNLHCKGCYARAHHRPTAPELPVSRWAELFR
                     EANELGISVIMLAGGEPLTRPEILELTRDYPEIIFPVFTNGLLIDQNLLTELRRRKNI
                     VPVISIEGFKAETDSRRGTGVFDHVRQAIMAVKQSGLFFGVSITVTRENFDNVTSEQF
                     IGDLTASGSRLFIFVEYTPVEAGTEDLILTPEQKTALARAMQIFPTRYRGVFTAFPGD
                     EEAYGGCLAAGRGFVHVSAEGNLEPCPFAPYSDTNLEHLSLKEALSSELLQAIRDQHE
                     RLKETQGGCALWENREWVGELMKRHEARGMRHENVGDSMKS"
     gene            complement(3060..3629)
                     /locus_tag="VF531_00685"
     CDS             complement(3060..3629)
                     /locus_tag="VF531_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7712507.1"
                     /translation="MDHTDLDAKTKIIHATKDFLDQGVDPDKITVRKIAEQAGVGVGL
                     INYHFQTRDNLLGQVVADTMSGMASAWQQMKVQPGADSLGKLKRMLKELSDYGAKYPK
                     LMQFMVKHELIQGNFSAEFYLLPVLREVFGKAKEELELKLIATALVSAMQGALVKDKA
                     FQRYAGVDIYDPEQRNETIDRLIDCLIIK"
     gene            complement(3878..4702)
                     /locus_tag="VF531_00690"
     CDS             complement(3878..4702)
                     /locus_tag="VF531_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712508.1"
                     /translation="MRGIVAILGRNLLNFTRDRMRFVFTLIMSAFFLFVFSFGMKSSM
                     AGIRQPMNYLISGVIIMTVFQQTLNNSTEILNDIASGFMKEILVAPVPRWQISIGQIL
                     SASIIGVVQGLIVLVASLFLGLKLDIGHFLEMVGIMAVTGLTFGSIGLFLATVAKGST
                     AFQIMTAVLVMPLTFLSGAYIPTTIMPGFLRPVTYINPLTYVTSIFRYIAMKMEHLSV
                     PELVNQGVAFNLHGFIITPGLGFCFIILMGAVFFGLCVRKFNRADFSTVKVFQHRH"
     gene            complement(4702..5619)
                     /locus_tag="VF531_00695"
     CDS             complement(4702..5619)
                     /locus_tag="VF531_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7712509.1"
                     /translation="MTASIIAVKDFTKKYGDFAAVDNISFEVEEGAIFAFLGPNGAGK
                     STTINTLCTILDKTGGELFINGHDVSKQKDKVRSDIGIVFQESTLDSKLTVEENLKFH
                     CKFYNVPDKEVSDRINFVLDLVDLQDWRKAPIGGLSGGMKRRVEIARGLVHYPKVLFL
                     DEPTAGLDPQTRANVWNYIYKLQKQRNITIFLTTHYMDEAEISTSVAIIDHGKLAAFG
                     TPEELKSKFSMSQVKVKTSRTEALAEYLNRNEIRYQKTEDCLNIYSKDTHEIIDILTA
                     FKESILDFEATKGSLNDVFLKVTGREIRE"
BASE COUNT         1453 a         1379 c         1456 g         1482 t
ORIGIN      
        1 cccccaggaa tacctgattg agtaccggat cgccaaggca tgccgcttga tggcggaaaa
       61 ggggctgacc atcggtgata tcgcccgttc cgtaggttat caggatccct tgctgttttc
      121 gaagaccttc aagcaaatca agggagaatc gccccgggtg tatcgtaacc ggatggatgg
      181 gcaggccgat gctaaaaatg gttattagtt attggttact agtgattagt aataataagt
      241 agtgatattc gtattagcaa ggtttgatta atcggagtgg aaatattttt cccggtttta
      301 gcagcaggat tatggagtga gacagtgaat acttaggcgt tttagcatta acgaattact
      361 aataactaat cactaataac taatcactaa taactaatca caagaattta agggggtttg
      421 ttatgtttaa cattgcattt tttcaacaat tctctctggg ggaacggttt ctctggtttg
      481 tgggattatc cgtcgcaggt tggatcttga gtaaactcct ggtccttgtt tgggagaaac
      541 ttattctgcc gctggcctcc aagacccaaa gctcgcttga tgatcaccta gccaagaatt
      601 tgcataaacc cgtcatccgg ttgctaatct tgggctcgat ttatttggcg gctaaattta
      661 ccctgctcac ggcggaacag gtaaaaatat atgtggcagc cgcggagaat ctgatctacc
      721 tgattttgat cttattcgtg gcgagtctgc ttaataatat tcttaagagt atcattgact
      781 ggtatctcca ggatatggcc tccaagaccg agtcaacgct ggatgatacg atattcccgc
      841 tatttcgtaa ggccggtgcg gtgatcatct acttcatcgc cagcaccgtg atattagaca
      901 agttcaacgt gaatctcacc ggtttcctgg cgaccgccgg tgttgcctcc ctcgcgttag
      961 cctttgcagc acaggaaacc ttatcgaatg tgatcgccgg aatcagtatt ttactcgatc
     1021 actcttacca tatcggcgac cggatcgagt taaaggatgg cctggtgggg gatgtaagcg
     1081 agatcggctt acggagtacg aaaattctct ctttggatca acggctcatc atcgtgccga
     1141 ataaggatat cgcgggttcg cgattgatta actggtgtca gccgaatgcg gccaccaata
     1201 ttaagttgaa gatcggcgtg gccatggatg aggacctgga aaaggtgaag cgggtggtca
     1261 ccgaggtctg caaccaagaa gcttctttat cgcgcaaggc cccggcggca gtggtatgca
     1321 ccggatttgg cccgtacttc atcgagctgc tggtagtggc tactgtggat gactgccgta
     1381 ctaccggaac agccaccgat catctggtag tgaagatcca ggaagcattt caacgtgaaa
     1441 agattcgcct gccttacccg ctgcaacaga tccagatgca gaaggtgggt taaggcaaca
     1501 gggaacgggc aataggcaac aggtaaaagg gaaaagtgga aagggaaaag ataaaaaaga
     1561 gtgagtcggt tatgtgtgtc tacgactcac tctcgctctc tctggcggca agcgccagag
     1621 agagtaaccc tcaaccttcg cagtcttttt cccaccttga atccaccttt tcccttcttt
     1681 cccgcttgtc ggggagagaa gggactaagg gttgagtcgc tttaagtttc atgctaagac
     1741 ttcattgaat ctcctacatt ctcatgcctc atgcctcgcg cctcatgcct tttcattaat
     1801 tccccaaccc actcccggtt ctcccataag gcgcagccgc cctgggtttc ctttagccgt
     1861 tcgtgctggt cacggatggc ttgcaataat tcggagctga gcgcttcctt cagggataga
     1921 tgttccagat tggtatcgga gtatggcgca aagggacagg gctccaggtt gccctcggcg
     1981 ctgacatgga caaatccccg tcccgccgcc aaacaaccac cgtaggcctc ttcgtctcca
     2041 gggaaggcgg tgaatacccc ccggtagcgg gtaggaaaga tttgcatcgc ccgggccagg
     2101 gccgtttttt gttcgggggt taaaatcaaa tcctcggttc ccgcctcgac cggcgtatac
     2161 tccacgaaaa tgaagagccg gctgccggag gcggtcaagt ccccaatgaa ttgttcactg
     2221 gtgacattgt cgaagttctc ccgggtcacg gtgatcgaga ctccaaagaa aagaccgctt
     2281 tgtttgactg ccatgatggc ctgacggaca tggtcaaaga cccctgtgcc acggcgggaa
     2341 tcggtctcag ccttaaaccc ctcgatgctg attaccggca cgatattctt gcgtcgccgc
     2401 aactccgtaa gtaaattctg atcgatcaac aacccattgg tgaaaaccgg aaagatgatc
     2461 tccggataat cccgggtgag ttcgagaatc tccggccggg taagcggttc cccgcctgcc
     2521 agcatgatca cggagatccc gagttcattc gcttcccgga agagttcggc ccagcggctc
     2581 accggtagct caggggcagt ggggcgatgg tgcgcccggg cataacagcc tttgcagtgc
     2641 agattgcagc cattggtgat gctggcgatg aggtaaggcg ggacgtgaat tccccgtctt
     2701 tcccagctac ggcgggaccg ggccgcttta tgtaaccgta aggtactgcg caaaataaat
     2761 aaggccagtg acgggttttg gaaggaaatt ttcagtgccg tggtaaagac ctggtttaac
     2821 gagttatcca tccgttcgat atagtctttg ggttcgctca ttgatgatgc tcctcataga
     2881 gatttgaaaa tcatattgca aagaaagatc attttgattt tgattaagaa tccgctatgt
     2941 cttgaaaatg gtgattttgg tgagtaaaga gtttctatct gtaagccagt tttattcaca
     3001 acgaaacaca caaaagacac gaaaatggga ataactttaa ttgcggctat tgaagtattc
     3061 tattttatga tcaaacagtc aatcaggcga tcgatggtct catttcgctg ctccgggtca
     3121 tagatatcca cccctgcgta acgctggaaa gccttatcct tgaccagcgc cccctgcatc
     3181 gcgctgacca gcgccgtggc gatcaatttc agctccaact cctctttggc ttttccgaaa
     3241 acctcccgca ggaccgggag aagatagaac tccgcggaaa agttgccttg gatcaattcg
     3301 tgcttgacca taaactgcat caatttggga tatttcgccc catagtccga aagttccttc
     3361 agcatccgct ttaacttccc gagcgaatcc gcaccgggtt gaaccttcat ctgttgccag
     3421 gctgaggcca ttccgctcat ggtatcggcc actacctgac cgagcagatt atcccgggtc
     3481 tgaaaatggt aattaatcaa cccaactccc accccggcct gttccgcaat cttacggacg
     3541 gttatcttgt cgggatccac cccttgatct aaaaagtcct tggtggcgtg gatgattttc
     3601 gtcttggcat ccaggtctgt gtggtccatc ggcatccacc tcttgaatgt gatttgaaca
     3661 tgttcaatat accataatac ggctgaaaag gcaaggaata ttttgagggt ttggtagaat
     3721 tcaatccgat ggctggatga ttcatgaggt taaaaatatg gagcggagat cgttttgatc
     3781 tccgctccaa aagaatcgat acagttcaat gaagttttac aggtttgatt tcgcgtgtag
     3841 gaacgtgaat ccatcgctcg gcacctgcac gaggagttta gtgacggtgt tgaaatacct
     3901 tgacggtaga aaaatcagcc cggttaaact tgcgaacgca taacccaaag aagactgcac
     3961 ccatcaggat gatgaagcag aaaccgagtc cgggagtgat gataaagccg tgaaggttaa
     4021 aggccacccc ttggttaacc agctccggga cagaaagatg ttccattttc atggcgatat
     4081 acctgaagat cgaagtgaca taagtcaacg ggttgatata agtcaccggc cgcaggaacc
     4141 ccggcatgat ggtggtggga atataagccc cggagagaaa ggtgagtggc atcaccaata
     4201 ctgcagtcat gatctgaaag gcggttgatc ctttggccac cgtcgccagg aaaagcccga
     4261 tcgagccgaa ggtcaggccg gtcacggcca tgattcccac catttcgagg aaatggccga
     4321 tatccagttt taaccccagg aagagactgg ccacgagcac aatgagtccc tggactacac
     4381 cgattatcga tgccgaaaga atttggccga tggagatctg ccaccgtggt acgggagcca
     4441 ccaggatctc tttcatgaag ccgctggcga tgtcgttcaa aatctccgtt gagttattga
     4501 gggtctgttg aaagacggtc atgatgataa ccccggatat caagtagttc atgggctgcc
     4561 ggatgccggc catggatgat ttcattccga atgaaaagac aaacaaaaag aaagccgaca
     4621 tgatgagggt gaaaacaaac cgcatccgat ccctggtgaa atttaaaaga ttcctgccta
     4681 aaatggcaac aattccgcgc attattctct gatctccctt ccggtcactt ttaagaagac
     4741 atcatttaaa gatcccttgg tggcttcaaa atccaggatg ctctctttga aagccgtaag
     4801 gatgtcgatg atctcatggg tatcctttga gtagatgttt aagcaatcct ccgttttctg
     4861 atagcggatc tcgttacggt tcagatattc cgccagcgcc tcggttcgtg aggtcttgac
     4921 cttgacctgg gacatcgaaa attttgattt gagctcttcc ggcgtaccga aggccgccag
     4981 cttgccgtgg tcgatgatag ccacgctggt gctgatctcc gcttcatcca tgtaatgggt
     5041 ggtcaggaag atcgtgatgt tgcgctgctt ttgtagtttg tagatataat tccagacgtt
     5101 ggccctggtc tgcggatcca atcccgccgt gggttcatcc aaaaagagta ccttgggata
     5161 gtgtaccaac ccccgggcga tctcaacccg ccgtttcatg cctccggaaa ggccacctat
     5221 cggtgctttc ctccaatctt gaaggtcgac aaggtcaagg acgaaattga tgcggtcgga
     5281 aacctcttta tcagggacat tgtaaaattt acaatgaaat ttgaggttct cttcaaccgt
     5341 caatttggaa tcgagggtcg attcctgaaa gacgataccg atgtcgctgc ggaccttatc
     5401 tttttgcttg cttacatcgt ggccgttgat gaatagctcg ccgccggtct tatccagaat
     5461 ggtgcataag gtgttgatgg tagtgctttt cccggcaccg ttggggccca gaaaggcaaa
     5521 gatggcaccc tcctccactt caaacgagat attgtccacc gcggcaaaat cgccgtattt
     5581 cttggtaaaa tctttgactg caatgatact cgctgtcatg aaataacctc cttttcccgg
     5641 ttcagcccca attataaaag aaggttttaa attttacgga atgggcgctt aaaatttata
     5701 taaaagagta taaaaagttt ataaagtcct cgaattgtcc cgaaagttgc aacagtagcg
     5761 gggtttataa
//
LOCUS       DASVMB010000022        10652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_445005,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000022 DASVMB010000000
VERSION     DASVMB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445005"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF531_00700"
     CDS             <1..309
                     /locus_tag="VF531_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7712510.1"
                     /translation="ARGVLDRLAELLFKTGSVIRVEGYTDNVPIHTARYQSNWQLSTD
                     RATNVIMYWLSKYPEQSPNLSAAGYGEYHSIAPNETNEGRAANRRVEIVVLRRSNPNK
                     "
     gene            331..999
                     /locus_tag="VF531_00705"
     CDS             331..999
                     /locus_tag="VF531_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712511.1"
                     /translation="MTAKYTSNKPQPFLWFLTGIFTLVLIFSQKTQVQAEIFHLNPEA
                     AYGMVTGTTDSKSLSVVYLDAESNLILIRPGIALGYGQNGEYRYTMVNPRIGAEFGLL
                     DYKLRCFETYQIRIVSETNGVTHTFSGFALGIGGEWKISEMLTFEGEYYLPQSAAYFR
                     NGNLNNSAHPSLEYWKVGARLTPLPLVSITFDYRKSGADAGLGGDNYQTDLNSYGLGV
                     RVGL"
     gene            1150..1368
                     /locus_tag="VF531_00710"
     CDS             1150..1368
                     /locus_tag="VF531_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7712512.1"
                     /translation="MKEQVTEALDKIRPSLQADGGDVELVDVVDGIVKVRLKGSCAGC
                     PMSTMTLKNGIERYLKQAIPDVKSVESV"
     gene            complement(1540..1998)
                     /locus_tag="VF531_00715"
     CDS             complement(1540..1998)
                     /locus_tag="VF531_00715"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX7712513.1"
                     /translation="MHIVLYQPEIPQNTGNIARLCAVAQVELHLIEPFGFFWDDEKLK
                     RAGLDYWDLVNIERHLNFQAYLDKYPGEHRYYASTKGKHFYHDFRFESNAAIVFGPET
                     RGLPPDILSRDPGHNIRIPMSSETRSLNLANSVAIILYEALRQQGFPGLI"
     gene            2311..3606
                     /locus_tag="VF531_00720"
     CDS             2311..3606
                     /locus_tag="VF531_00720"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX7712514.1"
                     /translation="MDYDQALSYIHECSKFGTKLGLERIREILRRLGNPQEHFRAVHI
                     AGTNGKGSTAAMLDSVLQAAGYKSGRYTSPHLSSYRERFTVNGEMISKNQLAAVITQI
                     GPVLEAVERDGFGAPTEFEVGTAIAFTHFAQEQVELAVIEVGMGGRLDATNVLTPVLS
                     IITHLALDHQQYLGDTLEQIAFEKAGIIKPGVPVVIGIQEPQIEKYLGEIAALRQAPC
                     RRASELELRDLVIGETGTSGTFLGTNFGPLKLDLKLIGRHQALNCLNVLAGVEMLNTA
                     GISINPDQLRSGLAGSVWPSRMERLEMFAPVKLYLDGAHNPDGAQTLAVTMNILYPKE
                     KASLLVGILNNRPLEELARIFSGFASNVITTSVPDPKSSDPVKLAQIFEGYGVPAVSE
                     PDPCRALDRLIATGNQLAVVTGSLYLTGYLRGFILHTGD"
     gene            3606..4280
                     /locus_tag="VF531_00725"
     CDS             3606..4280
                     /locus_tag="VF531_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712515.1"
                     /translation="MMPHRLTIICGAYGSGKTEFAIAYALKLKENSTAPVGLVDLDIV
                     NPYFRSRDITEELGKQGLTVISTEPGLEYSDMPALSPRIFATLQDRRYQVVFDVGGDP
                     AGARALGRFNRYFVEESYNLWIVINPFRPDTRTTEATIGMIQRLQEAGRLRASGIISN
                     INLGSETDWELWNKGLQQVTKVADAVQLPIVYQAVEAGFLEAHRPRLDAYPVFPLHLK
                     MLVPWA"
     gene            4429..4632
                     /locus_tag="VF531_00730"
     CDS             4429..4632
                     /locus_tag="VF531_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7712516.1"
                     /translation="MAKVVFNEERCKGCELCINACPKKIIHLADHFNTNGFRPAEVTN
                     TGECIGCAFCARMCPDVVIEVYK"
     gene            4647..5717
                     /gene="vorB"
                     /locus_tag="VF531_00735"
     CDS             4647..5717
                     /gene="vorB"
                     /locus_tag="VF531_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX7712517.1"
                     /translation="MAERMMMKGNEAIAEGALRAGCRFFFGYPITPQTEVPEYMAKKM
                     PEVGGTFIQAESEVAAINMVYGAAGAGARAMTSSSSPGVSLKMEGISYIAAAELPCVI
                     VNVARGGPGLGGLTPSQADYFQATKGGGHGDYRLIVFAPASVQEIIDLMLQAFITADR
                     YRNPAMILADGLLGQMMEPVTLDEIGDLQIPAKPWATTGMVSGKPQSLINSLGLAGED
                     LKKINQRLQQKYREIEAREVRFEEEGIQDADLVIVAYGTSARVAKSAIAMARTAGKKV
                     GLFRPITLWPFPTERIAQLSQAGKRLLVVEMSAGQMVDDVRLASGGRTQVDYFGWLNG
                     SLPSVTDVYDAIMKFASDRGVA"
     gene            5717..6466
                     /locus_tag="VF531_00740"
     CDS             5717..6466
                     /locus_tag="VF531_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7712518.1"
                     /translation="MEKVFGRPESLRDFPMHYCPGCTHGIIHRLVAEAIDDLHVREKT
                     IGVAPVGCAVMAYNYFNCDFQEAAHGRAPAVGTGIKRVLPDHVVFCYQGDGDLASIGT
                     AEIVHAANRGEKITVIFINNAIYGMTGGQMAPTTLPGQKTTTCPQGRDVNTMGHPIKM
                     TELLNTLEGPAYLARVSVHNPQHVMKAKEAIRKAFEVQIAGQGFAMVEVLSSCPTNWG
                     MAPQEALKWLEANMIPQYPLGVVRAPKEVAK"
     gene            6475..7011
                     /locus_tag="VF531_00745"
     CDS             6475..7011
                     /locus_tag="VF531_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX7712519.1"
                     /translation="MNLEAIFAGFGGQGIMLIGQLLAYSGMYEGKQVCWFPSYGPEMR
                     GGTANCSVVVSDSPVGSPVIAAPSVVVAMNRPSMERFESIMKPGGILFYNSSLIDVRP
                     KRTDIQVVAVPANDIASDLGNVKVANMVIMGALLQATGAVKLETVLSVLKKTMTGKKQ
                     ELLPLNKAALEKGMGLVG"
     gene            complement(7083..9074)
                     /locus_tag="VF531_00750"
     CDS             complement(7083..9074)
                     /locus_tag="VF531_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HEX7712520.1"
                     /translation="MRWKAIYLILGIFLLLGLFYYFFLDRVLEGSIERSLETVTGAKV
                     EVDKLHLNLSDLTLTIGKLQIANPHDTWRNLIETGPIRVKLAWEPLFSGKVVMDDISL
                     AGLMLDTPRRTNGRLGRSLLPGPFGKAQAKLNRDIASMPLLDSGSMQQEVNLNKDRLL
                     SSYRFQTSLSTEIIQERINNAGRNWTANLARLDETKQQLQQIDARLRELKTVTIKSLP
                     DLKQNLKTVEDLNRTIGTLGPQLKEVQSNFQNETTDIGRDVSDLASKADHDYHELLNL
                     AKLPDFDKINIAEILFGEALLNESSTFINLVDRLQAYVPAGILANQTSPAATPETTAR
                     NAPRHPRGGQDIIFPGRRTYPNFLIRHLAVSAQGPSRTFLKGYSANASLEGFTTEPAL
                     YGQPMEMSLSAASPCHSNLSLEGSLNHITNRIDDRFNLKIQNISLPAIDFMDRPYLPD
                     RISLGNANLFTDLAVESDKFDLHIVLDSPNLKWDFGAPGRDHHSASGNLTAEILQQTL
                     AGLDRLTIDYRLTGQNDQLRMSVSSNIDRLLKERFDAAIGAKVAQFKKELQLEVNRLL
                     DAKQKELKSQIAQYQTEFSAKFNEVQSYITREEQMIAAEQRNLEAKIKAEEDRLTNKA
                     AQTEQELKEQGQKELDKKQQQLEEELNKLKNQLLPGGKF"
     gene            complement(9077..9601)
                     /locus_tag="VF531_00755"
     CDS             complement(9077..9601)
                     /locus_tag="VF531_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HEX7712521.1"
                     /translation="MFWFNLVSKIINVLHSDESPRSLAAGFALGAIVGLTPLWSLHNL
                     FVFCLIFLINVSIPAAFFGIFLFSGFAYLLDPIFHQIGYYLLVKVAFLKPCWTYLYNI
                     PIAPLFRLNNTVVMGSLVTALLIFIPNYLGFTQVVCLYRLHFKERVDHWKVIQFLKAS
                     NLYQSYLKIRAMGV"
     gene            9828..>10652
                     /locus_tag="VF531_00760"
     CDS             9828..>10652
                     /locus_tag="VF531_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX7712522.1"
                     /translation="MRVFFIGRRFLPWVVGGMTVSLLWLGFWQGRILYRLTLAQPTTV
                     RIALVIDDFGGYAAGVQAMMDLPFPLTFAVMPFEEFAGKQAEQAIAKGHEVLVHLPFE
                     AYSADPSWYGKYYITTSTPGPEISRLIQEAFRILPMATGMNNHMGSLATGDSRVMGQV
                     LTELSKLNRFYLDSCTAKNSPVPQLVSRQNLPLIQRDIFLDEQAGENWIRSRLRDLVE
                     RARSRGQAVGIGHVGPGGPLLARILREEIPVYQKQGCQFVHLSELVHAKLIKPSSSI"
BASE COUNT         2578 a         2503 c         2880 g         2691 t
ORIGIN      
        1 gcccgggggg ttctcgacag gctggccgag ttgctcttca agaccggaag cgtcatccgg
       61 gttgaaggtt ataccgataa tgttccgatt catacggcgc ggtatcagtc caactggcaa
      121 ctctccaccg accgcgccac caatgtcatc atgtattggc tctcgaaata tccggaacaa
      181 tctccaaatc tttctgcagc cggatacggc gagtatcatt cgatcgctcc gaatgagacg
      241 aatgagggtc gggcggctaa ccgccgggtt gagatcgtcg tattgcgccg atccaatcca
      301 aacaaataaa gtacattttg ggggatggct atgacagcaa aatatacatc gaacaaacca
      361 caaccatttc tatggttcct aacgggaatt ttcacgctgg ttttgatttt ttctcaaaag
      421 acccaggttc aggcagaaat attccaccta aacccggaag ccgcttacgg aatggtaacc
      481 ggaacgaccg attcaaaatc attatctgtg gtttatctgg atgccgaatc caacttgatt
      541 ctcatccggc cgggaattgc gcttggatac ggccagaatg gcgagtatcg gtatacaatg
      601 gtaaatcccc ggattggcgc agagtttgga ttattagatt ataaactcag gtgttttgag
      661 acgtaccaga tccggattgt gagtgaaacg aacggcgtaa cccatacttt ttccgggttc
      721 gcgctgggta tcggtggaga gtggaaaatc tccgagatgc ttacatttga aggagaatat
      781 tatcttcctc agagtgcagc ctattttcgg aacggcaatt tgaacaattc agcccatccg
      841 tcccttgagt attggaaggt gggagcacga ttgacaccgc tccccttagt cagtataact
      901 tttgattatc gtaaatcagg cgctgatgca ggtctgggcg gggataatta tcaaaccgac
      961 cttaacagtt acgggctagg ggtacgggtc ggattataaa atgatcatga ttccttagct
     1021 ttcaggttaa tttttgacga tctttattgc caagggcggt aaactctgtt aagataaaag
     1081 cgcggtattg gatatagact aatgaaaaag tatcccgatc gctcgggaat gaagaaaaga
     1141 ggttgcccta tgaaggaaca agtgaccgaa gcgctcgata agattcgtcc ttcgctgcaa
     1201 gcggacgggg gcgatgttga attagtggat gtggtggacg ggatcgtcaa ggttcggctg
     1261 aaaggttcct gtgccggttg cccgatgtcg acgatgaccc tgaaaaatgg aattgagcgt
     1321 tatttgaagc aagccatacc ggatgtaaag agcgtagaat cggtttaaac acggagagta
     1381 taaaaaccgt tctctgaaaa gaggacggtt tttttatagg caacagggaa tagggaacag
     1441 gcaacaggta aaaccctatt gcctgttgcc cagtgcctat tgcctgttct tgcgcctcac
     1501 gcctgttccc ctattgcctg ttgcccagtg cccgttgcct tagatcagtc ctggaaatcc
     1561 ctgctgccgt aaagcctcat aaagtatgat cgccaccgag ttggccagat ttaatgatcg
     1621 tgtttcacta ctcatcggga tccggatatt gtgcccggga tcccggctta ggatatccgg
     1681 gggtagaccg cgtgtctccg ggccaaagac aattgccgca ttactctcaa aacgaaaatc
     1741 atgatagaaa tgctttccct tggtggaggc atagtaacga tgttcccccg gatacttgtc
     1801 gaggtaagcc tggaagttca aatgacgctc gatgtttacc agatcccaat aatccaatcc
     1861 ggcgcgcttt agcttttcat catcccagaa aaaaccgaac ggttcgatca agtgcagctc
     1921 tacttgggct actgcgcata accgggcgat attaccggta ttttgaggta tctcgggctg
     1981 gtacaagaca atatgcatgt tgtttcctcc gttttgaagg gtataggtta ctcttttgtg
     2041 aacaatttga attattgtat tacatattta ccggaacgac cacgccggga aatcgtctgt
     2101 ggtttctttg gtgtcgcttg gcgttttccc ggaattgtga tacaataatc tttaaatatt
     2161 atagcatgaa attcatattt caagcggttt accggccaaa ttgacactgg aatcctgttt
     2221 tagcggctat caaccggtga cataaccggt ggtttaccca ctggttaaga tatttgtaat
     2281 gctcaagttt atcataagcg ggcgctgaaa atggattatg accaagcatt aagttatatt
     2341 cacgagtgtt ctaagttcgg aacgaagttg ggacttgagc gtatccggga aatcttgcga
     2401 cgactcggca atccccaaga gcatttccgg gccgtgcata tcgccgggac caatggtaaa
     2461 ggttccactg cagccatgct cgactccgtt ctgcaggcgg cagggtacaa atccggccgt
     2521 tatacctcgc cccatctaag ttcataccgg gaacggttta ccgtaaacgg cgaaatgatc
     2581 tccaaaaacc aacttgccgc agtgatcacc cagatcggtc cggtcctgga agcggttgag
     2641 cgcgatggtt tcggtgctcc tacggagttt gaggtaggta cggccatcgc ctttacccat
     2701 tttgcccaag agcaagtgga gttggctgtg atcgaggtgg gtatgggcgg ccgtctggat
     2761 gcgaccaacg tcctcacccc ggttttaagt atcatcactc atttggctct ggatcatcaa
     2821 cagtatttgg gcgacaccct ggaacagatt gcttttgaaa aagcgggtat catcaaacct
     2881 ggagtgccgg tagtcatcgg tattcaggaa ccccaaatcg agaaatatct cggtgaaata
     2941 gccgccttga ggcaagcgcc gtgccggcgc gccagtgaat tagagctccg ggacttggta
     3001 attggtgaaa ccgggaccag tggtactttc ctgggaacca atttcgggcc gcttaagctg
     3061 gatctgaaat taatcggcag gcaccaggca ttaaattgcc tgaatgtttt ggccggagtg
     3121 gagatgctaa atacagccgg catatccatc aatccggatc aactgcgaag cggattggcc
     3181 ggttctgtct ggcccagccg aatggagcgg ctggaaatgt ttgcccctgt caagttgtat
     3241 ctggatggtg cccataaccc cgatggagct caaacattgg ctgtgaccat gaatatactc
     3301 tatccgaaag agaaagcctc tttgttggta ggtatactca ataaccggcc tttggaggaa
     3361 ttggctcgga tcttcagcgg ttttgcttcc aatgtgatca cgacttcggt tcccgatccc
     3421 aaaagctccg acccggtcaa actggcgcaa atatttgagg gttatggggt accggcagtt
     3481 tctgagccgg atccttgccg ggctttggac cgtttgattg ccaccggaaa tcagctggct
     3541 gtcgtgaccg gttcattata tcttaccgga tatttacggg gctttatttt gcatacggga
     3601 gactgatgat gccacatcgt ttgaccatta tttgcggcgc gtacggcagc ggcaaaaccg
     3661 aatttgccat cgcctacgcg ctgaaattaa aagaaaactc cactgccccg gtaggactcg
     3721 tggatctgga tatcgtcaac ccctattttc gttcacgaga tatcaccgaa gagcttggca
     3781 aacaagggct aaccgttatt tccaccgaac cgggactgga gtactccgat atgccggcgc
     3841 tttcaccacg tatttttgcc accttgcagg atcgccgtta tcaggtggtc ttcgatgtgg
     3901 gcggggatcc tgccggagcc cgggctttgg ggcgattcaa ccgctatttt gtggaagaat
     3961 cttataacct gtggattgtc atcaatccat tccggcccga tactcgcact accgaggcta
     4021 ccatcgggat gattcagcgg ttgcaggaag ccggtcgctt aagggccagt ggaatcatat
     4081 caaatattaa tcttggttcg gagacggact gggaactttg gaacaagggg ttgcagcagg
     4141 taacaaaagt ggcggatgca gttcaattgc ccattgttta tcaggcggtt gaggccggtt
     4201 ttttggaggc gcaccggccc cgattggacg cttatcccgt ttttccactc catctaaaaa
     4261 tgcttgttcc ctgggcataa tgttgactcc ctgccatatc gttcataagg ccggttgacc
     4321 gggcgggcaa tctcgaaaac cgcttggtct tgtcaaaccc ggttaaaaag tttatgatta
     4381 ttatcaggta ataataattg taaagataac atttaaggag gcagatctat ggccaaagtc
     4441 gtctttaacg aagagcgttg caaaggatgc gagctatgta tcaacgcttg tcccaagaag
     4501 atcattcatc tggccgacca cttcaatacc aatggtttca ggccggccga ggtcaccaat
     4561 acgggcgagt gtatcgggtg tgctttctgt gcccggatgt gccccgatgt cgtgattgaa
     4621 gtatataaat aaaggaggtt aaataaatgg cggaacgaat gatgatgaaa ggcaatgaag
     4681 ccatcgctga gggcgcgctc cgggccggtt gccgcttttt cttcggttat ccgatcacgc
     4741 cccagactga agtcccggaa tatatggcca agaaaatgcc ggaagtgggc ggtactttca
     4801 tacaggcgga gagtgaagtc gccgcgatta acatggttta cggtgcagca ggagccggag
     4861 ccagggcgat gacctcctcg tccagtccgg gggttagcct aaaaatggag ggtatctcct
     4921 atattgctgc cgccgaattg ccttgtgtaa tcgtaaacgt agcccgtggt ggtccggggc
     4981 tgggcggact caccccttcc caggcagatt attttcaggc aacgaaaggc gggggacacg
     5041 gtgattaccg gttaatcgtc tttgccccag cttcggtaca ggagattatc gatctgatgc
     5101 tgcaagcatt tattacagcg gatcgttacc gcaatcccgc catgatcctg gccgacggtt
     5161 tgctgggcca gatgatggaa cccgtgacgt tagacgaaat aggcgattta cagatccctg
     5221 ccaaaccatg ggctacaacc ggaatggtct cgggaaagcc ccagagtttg atcaatagct
     5281 tgggactggc aggggaagat ctcaaaaaga ttaaccaacg gttgcagcag aaataccggg
     5341 agattgaagc gagggaggtc cgattcgagg aagaaggcat ccaagatgcg gatctggtca
     5401 tcgtagccta cggtacctcg gcccgggtag ccaagtcggc cattgccatg gcacggacgg
     5461 cgggtaaaaa agtcggtctc ttccgcccga ttaccctctg gcccttcccg acagagcgca
     5521 tcgcccaatt aagccaggca gggaaacgac ttctcgtagt ggagatgagc gccggccaga
     5581 tggtggatga tgtccggctg gcatccggtg gtcggactca ggtggattat ttcgggtggc
     5641 tcaacggttc gttgccgtct gtaaccgatg tttatgatgc gatcatgaaa tttgcttccg
     5701 acaggggggt ggcctaatgg aaaaggtatt tgggagacct gagagtttac gcgattttcc
     5761 gatgcattat tgtcccggtt gcactcatgg aatcattcac cgcttggtag ccgaagccat
     5821 cgatgatctg catgtccggg aaaaaaccat tggagttgcc ccggtgggct gcgcagtgat
     5881 ggcgtataac tatttcaatt gtgatttcca agaggcggcc catggccgtg ccccggcagt
     5941 gggaaccgga attaaacggg tgctcccgga tcacgtggtc ttctgttatc agggcgacgg
     6001 cgacctagct tccatcggaa ccgccgagat cgtacacgcc gccaatcgtg gtgaaaagat
     6061 tactgtaatc ttcattaata acgctattta cggtatgacc ggcggccaaa tggctccgac
     6121 caccctgccg gggcagaaga caaccacctg tccgcagggt cgtgatgtga ataccatggg
     6181 acatcccatc aaaatgactg agctcttgaa tacgctggaa ggtccggctt atttggcgcg
     6241 ggtcagtgtg cacaacccac agcacgtcat gaaggcgaag gaagcgattc gtaaggcttt
     6301 tgaggtacaa atcgcgggac agggttttgc catggtggaa gttctttcga gctgtcccac
     6361 caactggggg atggcgccac aggaagccct aaaatggttg gaagccaata tgattcctca
     6421 atacccgctg ggtgtggttc gggcacccaa ggaggtggca aagtgagcga agctatgaat
     6481 ctggaagcga tttttgccgg cttcggcggt cagggtatca tgcttatcgg tcaactttta
     6541 gcctattcag ggatgtacga gggcaagcag gtatgctggt tcccgtcgta cggaccggag
     6601 atgcggggcg gcaccgccaa ctgttcggtc gtcgtatcgg acagtccggt cggctcgccg
     6661 gtcattgcgg ctccatcggt ggtggtggcc atgaaccggc cttcgatgga acggtttgag
     6721 tcgatcatga agccgggcgg tatccttttt tacaattcat ccctcatcga tgtcaggccg
     6781 aaacggaccg acattcaagt agtagcggtt cctgccaacg atattgcaag cgacttgggg
     6841 aacgtaaagg ttgccaacat ggtaatcatg ggtgcgctgc tccaggccac cggtgcggtg
     6901 aaactggaga cggtactttc ggttttaaag aagaccatga ccggaaagaa acaagagttg
     6961 ttgcccctga ataaagccgc cctggagaag ggcatgggtt tggtgggata aatacttttt
     7021 cctacagcaa aagcccccgg caggttgaag tgccggggct ttttcatttt atcaaacggg
     7081 atttagaact tgccgcccgg taacaactgg ttcttgagtt tgttcaattc ttcctccagt
     7141 tgttgttgtt tcttgtcgag ctctttttga ccctgttcct ttaattcctg ttccgtttga
     7201 gccgccttat tcgtcaacct atcttcttca gcctttattt tagcttccag gttccgctgt
     7261 tctgcggcaa tcatctgctc ttcccgggtg atatagcttt gtacctcgtt aaactttgct
     7321 gaaaattcag tctgatattg ggctatctga ctcttcagtt ctttttgttt ggcgtccagg
     7381 agccggttga cttccagttg tagctccttt ttaaactggg cgactttagc gccgatagcc
     7441 gcatcgaatc tctccttgag taaccggtcg atattggaag agacactcat tcgcagttgg
     7501 tcgttttgac ccgtaagacg atagtcgata gtcagccgat ccaatcccgc cagagtttgc
     7561 tggaggatct cggcggtaag attaccggat gcggaatgat ggtcgcgccc gggcgcgcca
     7621 aaatcccatt ttagattggg actgtctaaa acgatgtgga gatcgaattt atcactttcc
     7681 actgcgagat cggtaaagag attggcattt cccaacgaaa ttcggtccgg caggtacggt
     7741 cggtccataa agtcaatcgc gggaagactt atgttttgaa tttttagatt gaaacggtca
     7801 tcgatacggt ttgtgatatg gtttaaactg ccttccaggc ttaagttcga atgacaggga
     7861 ctcgctgccg atagggacat ttccattggc tggccgtaga gcgccggttc ggtagtgaaa
     7921 ccctcaagtg aagcattggc cgaatagccc ttgagaaagg tccgggaagg gccttgggcg
     7981 gaaaccgcca ggtgccgtat taaaaagttt gggtatgtcc tgcgtccggg gaaaatgata
     8041 tcctgtccgc cgcgcggatg acgcggcgca ttccgtgccg tcgtctccgg cgttgccgcc
     8101 ggagacgttt gattggctaa aatcccggcc ggcacatagg cttgcagccg gtccaccagg
     8161 ttgatgaagg ttgaggattc attgagcagt gcctcaccga ataggatctc ggcgatgttg
     8221 atcttatcaa aatcaggcag tttggctaaa tttaagagtt cgtgataatc atgatcggcc
     8281 ttgcttgcca gatcactgac gtcacgaccg atatcggtcg tttcattttg aaagttactt
     8341 tggacttctt tcagctgcgg gcccagagta ccgatggtcc ggtttagatc ctccacggtt
     8401 ttcagatttt gcttcaaatc aggcaggctc ttgatggtta cggttttcaa ctcccgtagc
     8461 ctggcgtcga tctgctgaag ttgctgcttc gtctcatcca gccgggccag attggcggtc
     8521 caattccggc cggcattatt aatccgttcc tgaatgattt ctgttgacag actggtttga
     8581 aagcgatagg aggagagtag ccgatcttta tttagattca cttcctgttg catgctgccg
     8641 gagtctaaga gcggcataga agcgatatcc cggttcaact tggcttgagc tttaccgaat
     8701 ggtcccggca ataaactgcg ccctagccgg ccgttggtcc ggcgcggcgt atccaacatc
     8761 agtccggcca gagagatatc gtccataacc accttgccgg agaagagcgg ctcccaagca
     8821 agtttgaccc ggattgggcc ggtctcgatc aggttgcgcc aagtatcgtg gggattggct
     8881 atctgtaact taccgatggt cagggtcaga tcggagaggt tcaggtggag tttatcaacc
     8941 tccaccttgg ccccagtcac ggtttccagg gaacgctcga tggaaccttc caaaacacgg
     9001 tcgaggaaga aataataaaa caaccccagt agtaaaaata taccaagaat tagataaatt
     9061 gctttccagc gcattttcag actcccatgg ctctgatttt caggtaggat tgatataggt
     9121 tgctcgcctt caggaactga atcaccttcc aatgatcgac ccgttcctta aagtgtaatc
     9181 ggtataggca gaccacttgc gtaaacccga ggtagttggg gataaagatt aacaatgccg
     9241 tgaccaggct gcccataacg acggtattgt tcaaccggaa caacggcgcg atgggaatgt
     9301 tataaagata ggtccagcat ggtttgagaa aggccacttt gaccagcaaa tagtatccaa
     9361 tctggtgaaa gatgggatcc aaaagatacg caaaaccact aaagaggaag attccgaaaa
     9421 aagccgcggg gatgctgaca ttgatcaaga agatcaggca gaaaacgaac aggttatgta
     9481 acgaccaaag cggagtcaat ccgacgattg ctcccaatgc aaaccctgcc gccaaactac
     9541 ggggagactc atcggaatgc agaacattga tgatcttcga gaccagattg aaccagaaca
     9601 tttaggatca cctcaaataa tccctcaaat gtcattacat ttcgcggcag gagcggcgct
     9661 ttcctgccaa tcaccgccaa aattcgttta ttccaccgca taaaaacaaa gacaacgaaa
     9721 cacaggaact tctcgaaaac gtgaacgggt gatgatttta tttcgtgtct tttcgtgtat
     9781 atataaagaa tggaaaggac ctgatttaat ggaaatgagg aggcggaatg agggtatttt
     9841 tcattggacg ccggtttctt ccctgggtgg tggggggaat gaccgtatct ctattatggc
     9901 tcggtttttg gcagggccga atactatatc gcttgacgct ggcgcaacca actactgtaa
     9961 ggattgccct ggtcattgat gattttggag gttatgccgc cggggtccag gcgatgatgg
    10021 atctgccgtt cccattgaca tttgcagtga tgccgtttga ggagttcgcc gggaaacagg
    10081 ctgaacaagc gatagccaaa gggcatgagg ttctggttca ccttccattt gaagcttaca
    10141 gcgccgatcc cagctggtat gggaagtatt atattaccac aagtactccc gggccggaga
    10201 tcagtcgctt gattcaggag gcgttccgga ttttgccgat ggctaccggg atgaacaatc
    10261 acatggggtc cttggctacg ggtgatagtc gggtgatggg gcaggttctg actgagttaa
    10321 gcaagttgaa ccgtttttac ctggatagtt gcacggcgaa gaactcaccc gtaccgcaat
    10381 tagtctcccg gcagaatctt cccttgatac aacgggatat ctttttggat gaacaggcgg
    10441 gtgaaaattg gattcgtagc cgtttgcggg atctggtgga gcgggcccga tcccgggggc
    10501 aggctgttgg aatcgggcat gtgggacctg gtgggccgct acttgcgcgg attcttcggg
    10561 aagagattcc tgtttaccaa aaacaagggt gccagtttgt tcacctttct gaactggtcc
    10621 atgctaaact catcaaaccg tcgtcctcga ta
//
LOCUS       DASVMB010000023         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_76960,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000023 DASVMB010000000
VERSION     DASVMB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76960"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..730)
                     /locus_tag="VF531_00765"
     CDS             complement(131..730)
                     /locus_tag="VF531_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712523.1"
                     /translation="MCDQCGEMEGLLISDNINLELIQKLVDDGKIDLYAGNCNPNELS
                     EYLDRGNHYTMNTYYHCKKCNKYYYVGFCLYGNPLIEEVDSQKILDDILRLEWGYVGT
                     YFKNGLTMKDAKYALQHAAYIIAPVWSCLMDAFGDGTVISMLALTKTLKVLYQRVQKD
                     GAIELKYPDGTEDQIINNETFDGIIQKYFSESVLHEIKR"
     gene            complement(731..1129)
                     /locus_tag="VF531_00770"
     CDS             complement(731..1129)
                     /locus_tag="VF531_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712524.1"
                     /translation="MADDVDISVYMEDIKKWCKDPERLLEYEKSIREAIKQNKKDDDS
                     LRTSASIPDDLWKGIVDAINYDSASTVGWAIKKLSIVYERIQLGERIFVPSIDAYLEK
                     SNFEAVICKTFSQFIFNEILELYRSRKEIR"
     gene            complement(1361..1831)
                     /locus_tag="VF531_00775"
     CDS             complement(1361..1831)
                     /locus_tag="VF531_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002064022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX7712525.1"
                     /translation="MKVKTESILPAPSDQRILEFESNCRITLPEEFKKFIKEYNGAVP
                     ETKVFFDGRSERMVERFLSLLDDFKSDDVNGWYDIEVVLSQIDTRLGDDEDLIGYNVI
                     PFAALFAGDFVCLDCRKSGIAPVVVWYHEESDDFSPVFSTVAASVTEFFEMLKE"
     gene            complement(2205..2477)
                     /locus_tag="VF531_00780"
     CDS             complement(2205..2477)
                     /locus_tag="VF531_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712526.1"
                     /translation="MKRIIVVALCMLVLSVQAFATDLVNKDSVKYRVEIKPDSYTRTE
                     MDVAASSTYSDKLKKGYVIKNLTTGETITVTTDKKVLIVDGKFKQE"
     gene            complement(2525..3955)
                     /locus_tag="VF531_00785"
     CDS             complement(2525..3955)
                     /locus_tag="VF531_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712527.1"
                     /translation="MKLDLSKMQAANGYFQGEPECISGALRNLPFEYKKRLSQSFLAI
                     LPEQITSKVSGSSFHVSQKIDGHLQLLVYNGEQAYIIGRGGIVRTGLPCLEQAAAGFR
                     EKGITSLIAAGELYYAKEDGRSRVYDVIAALADQGKLANLRIACFDILELNGESFKTA
                     NYETVYQKLTAILPPAGPVHVIPTEIVKSKEQVGQLYEKWVVEQGHEGIVVRSGMPFM
                     FKIKPKYTFDAVVVGYAEGINEHKGMVKSLLFAFRKGELYQVGGKVGNIPEEDRRKLY
                     EVFSQKHVASRYIETDNEGIAFHMVAPDTVIEISCNDLISETAYGKTLPNQVLMFSDQ
                     TYTLHQTTPGVKFINLVWERIRNDKANNDDDVRFSQISDLVYLPAGEPVVAELPQSQV
                     IFREVYRKEAKDKVMVQKFLVWQTNKEKADPRYPAYVMHYTNFSSQRKTPLEREVRIS
                     NSREQIMMLVKEFIAENVKGGWNLVS"
     gene            complement(3952..>4418)
                     /locus_tag="VF531_00790"
     CDS             complement(3952..>4418)
                     /locus_tag="VF531_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712528.1"
                     /translation="TGLMLEDTSRIYITRDNQLLYGVELYEIIRNPDGSEKERHYYTP
                     KLSNICNDLPVLCTGKKFPKAEAVKRFIFSRKYQIKHVNGLTYDFLYEIAGELDETKS
                     LMLVGGGKTGTDPLIMNDGGTPYRGFLEGRVNGDKYCLILHLTNLELKEISG"
BASE COUNT         1160 a         1080 c          824 g         1354 t
ORIGIN      
        1 aatatagtcc cgtggtatta tctatcccgg cgttgcacat attgttttga cttactttta
       61 gtattttata atccatattt acacttccga ttcaattcat tcatgggatg gcgaagcttg
      121 caatcaacgc ctaccgttta atttcatgca atacagattc ggaaaagtat ttttggatga
      181 tcccatcgaa tgtttcatta ttaattattt gatcttctgt tccatcggga tatttcaatt
      241 caatggctcc gtctttctga actctttgat ataatacttt taatgttttt gttaacgcca
      301 gcatcgatat gacagttcca tcaccaaatg catccatcag acaagaccat accggcgcta
      361 taatatatgc tgcatgttgg agcgcatatt tcgcatcttt catcgttaag ccatttttga
      421 aataagtacc gacatagccc cattccaacc tcaaaatatc atccagtatt ttctgtgaat
      481 cgacttcttc aatcagaggg ttcccataaa ggcaaaagcc tacataataa tatttgttac
      541 attttttaca atgataatat gtattcattg tatagtgatt gccacgatcc agatactctg
      601 ataactcatt cggattacag tttcccgcat acagatctat ctttccatca tcaaccagtt
      661 tttggattaa ttctaaattg atgttatctg aaatcaataa tccttccatc tctccacact
      721 gatcacacat ttaccgtatc tcctttcgcg accggtaaag ctccaatatt tcgttaaaaa
      781 taaattggga aaatgtttta caaattactg cttcaaaatt acttttttct aaataagcat
      841 ctatacttgg cacaaatatt ctttctccca attgaatccg ttcataaact atacttaatt
      901 tcttgatcgc ccatcctacg gttgaggcgc tatcgtagtt aatagcatca acaattcctt
      961 tccataagtc atccggaatc gaagcggatg tccgtaaaga atcgtcatct tttttattct
     1021 gtttgattgc ctcgcgaata cttttttcgt attccaataa tctttccggg tctttacacc
     1081 atttcttgat atcttccata tatacggaaa tatccacgtc atcagccatt ttttacctct
     1141 actttaaatt tccttattac atccttcatt gcttcaatcc ggcacattca ttttctcagt
     1201 aatcagaaac ccctctttta gaatgggttc accaaaagcc gccaaaaaat cgccactcct
     1261 agatactttc atgtgtatca acctctacta tggttaacgt tttgttcttt ggaattttat
     1321 cgtatctcat gcacaataca atcgggtaac gtttaaaccg ttattctttt aacatctcaa
     1381 aaaactctgt aacgctcgca gctacagttg aaaaaaccgg gctaaagtcg tccgattcct
     1441 catgatacca gacgactacc ggagcaattc cgcttttcct gcaatccaag catacgaaat
     1501 ccccggcaaa aagcgctgca aacggtatca cattatatcc gatgagatct tcgtcatcac
     1561 ctaatctagt atcaatttgc gacagaacca cttcaatatc gtaccatcca tttacatcat
     1621 ccgacttgaa atcatccaaa aggctcaaaa aacgctcaac catccgctca cttcggccat
     1681 caaagaatac tttcgtttcg ggtactgcgc cattgtattc cttaataaat tttttaaatt
     1741 cctcaggcag agtgatccgg caattggact caaactcaag aatcctttga tcgcttggcg
     1801 ccggcaagat gctttccgtt ttaaccttca tttttttccc ctttaagtct atctaaaaga
     1861 aatacaatag tcagcttgcg ccactccaac aattctgtgc cgtctggcgc aggagttggt
     1921 agttcctaaa tcaaagctca tattttatct taaacaaaat caattgttgg atattcactt
     1981 tcatagtgtt caagcatttc cttcggagct atgggatcgt ttatatcgat tttgatgccc
     2041 tttctcattg ccaactttac aaatcccctc ctatgctcta agacatcaag atttatgctc
     2101 gaaacgccct gatgatggca agctaccggt gccttctatc cgaaggatac aaggcaccgg
     2161 caatctcatc tcaaaattgt ttcagaaggt caagaaattt acgattattc ctgtttaaac
     2221 tttccatcca cgatcaaaac ctttttatcc gttgtcacag tgattgtttc accggtggta
     2281 aggttcttga tgacatatcc ttttttcaat ttgtcggaat aggtagaact tgcggcaaca
     2341 tccatttccg ttctggtata ggagtcgggt ttaatctcaa ctctgtactt gaccgagtcc
     2401 ttgttcacca gatcggtggc aaaagcttga acagagagta ccagcatgca taatgcgact
     2461 acgataattc ttttcatgaa gattctcctt tttttattga tagttgtcct actaaaacaa
     2521 gcgctcaact caccagattc catcccccct ttacattctc ggcgataaat tctttaacca
     2581 gcatcataat ctgctcccgg ctgtttgaga tgcggacctc ccgttccagc ggagtcttcc
     2641 gctgggaact gaaattggta taatgcatca cataggccgg gtagcgggga tcggcctttt
     2701 ccttattggt ctgccagact aggaactttt gaaccatcac cttgtctttg gcttctttcc
     2761 ggtagacttc cctgaaaatc acctggctct gaggaagctc cgctacgact ggttcgccgg
     2821 cgggaaggta aaccaaatcg ctgatctggg aaaagcggac atcatcgtca ttgttggcct
     2881 tgtcgttgcg gattctttcc caaaccaggt tgatgaattt cactcccggt gtagtctggt
     2941 gaagcgtata ggtttgatcg gaaaacatca ggacctgatt gggtaaggtt ttaccgtaag
     3001 cagtctccga aatcagatcg ttacagctga tctcgatcac cgtatccggg gcaaccatat
     3061 ggaaggcgat accttcgtta tccgtctcga tataacggga tgccacatgc ttttgggaaa
     3121 acacctcata aagcttgcgg cggtcctcct ccgggatatt gccgactttg cccccaacct
     3181 gatacagttc acctttgcga aatgcgaaca gcagggattt aaccattcct ttatgctcgt
     3241 tgatgccttc ggcatagccg acgacgaccg cgtcaaaggt gtacttgggc tttatcttaa
     3301 acataaacgg cattccgctc cgaacaacaa tgccttcgtg accctgttcc accacccatt
     3361 tttcatataa ctggccgacc tgttctttgg acttgacaat ttcagtggga ataacatgaa
     3421 ccggaccggc cggcggtagt atggcggtta acttctgata gaccgtctca tagttggcgg
     3481 ttttaaaaga ttcaccgttt aactccaaaa tatcaaaaca ggcaatccgt agattagcaa
     3541 gttttccctg atccgctaat gcggcaatga catcataaac ccggctccgg ccatcttcct
     3601 tggcataata taactctccc gcggcaatca gggaggttat ccctttttcc cggaacccgg
     3661 ccgcggcctg ttccagacag ggaagtccgg tcctgacaat accgcccctc ccgatgatgt
     3721 aagcctgttc cccgttgtat acaagtaatt gcaaatggcc gtcgatcttt tgagacacat
     3781 gaaaggacga accactgacc ttggaggtta tttgctcggg aaggatcgcc agaaaactct
     3841 gggaaaggcg tttcttgtat tcaaagggca ggttacgcag agcgccggag atgcattccg
     3901 gctcaccctg gaaatatccg ttggcggcct gcatttttga cagatcgagt ttcatccgct
     3961 aatctccttt agctccagat tggtaaggtg cagtatcaga caatacttat caccatttac
     4021 ccggccttcc agaaaaccac ggtaaggggt gccgccgtcg ttcataatca gcggatcggt
     4081 tccggtttta cctccgccga ccagcatcag cgacttcgtc tcgtcgagct ctcctgcaat
     4141 ctcatagaga aagtcgtatg taagtccatt gacatgtttg atttgatatt tgcgggaaaa
     4201 gataaaccgt ttgactgctt cggctttggg aaatttcttg ccggtgcaga gaacgggcag
     4261 gtcattacag atgttggata gtttcggcgt ataataatgc cgttctttct ccgaaccgtc
     4321 cggattacgg ataatctcat aaagttcgac cccatagagg agttgattat ccctggttat
     4381 atagatccgg ctggtatctt ccagcatcaa tccggtgc
//
LOCUS       DASVMB010000024         5868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_592010,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000024 DASVMB010000000
VERSION     DASVMB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592010"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(783..1439)
                     /gene="epsC"
                     /locus_tag="VF531_00795"
     CDS             complement(783..1439)
                     /gene="epsC"
                     /locus_tag="VF531_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX7712529.1"
                     /translation="MDHSDQMKSGSYWTGISRAFRELRYLGDDFRNAYHKDPALRGKP
                     LALLELITYPGLWAISMHRMAHLLYQIKVPFIPRLISQVSRFLTGVEIHPGARIGRGF
                     FIDHGHGVVIGETAEIGHDVLLFHQVTLGNASVAATGKRHPTIGDQVIIGAGAMILGP
                     ITVGDHSQIGAGSIVTKDVPSHSLVVGNPGQVIKRFGVKIVAPGCGERQQAIGVRHEA
                     "
     gene            complement(1505..2491)
                     /gene="gshAB"
                     /locus_tag="VF531_00800"
     CDS             complement(1505..2491)
                     /gene="gshAB"
                     /locus_tag="VF531_00800"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEX7712530.1"
                     /translation="MNEEYEGMELSTRVLIDEALRRGISVEVLDKNDNTIRLRKDSHI
                     EYIKQATKTSADSYIAPLLMENKQVTKQILRESGIKVPEGVTVHSVAEAEQVYPSFSG
                     KAFVIKPKSTNFGKGVKIIKACQSREDYCHSVKEAFQYDHEILIEEFVPGKEYRFLVI
                     GDEVPAVLHRVPANVKGDGIHTIEELVAEKNQDPLRGKGYVTPLEKINLGAEEAAYLK
                     LQGRDFHFIPQNDATVYLRENSNISTGGDSLDFTDIIPEGYKTIALAAARAVGAKICG
                     ADIIIQDLAAEPTERNYRVIELNFNPALHIHDFPFQGQNRRVEQKVLDLLGF"
     gene            complement(2505..4004)
                     /locus_tag="VF531_00805"
     CDS             complement(2505..4004)
                     /locus_tag="VF531_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7712531.1"
                     /translation="MNRNFSKNLASFSGPDQARLLLDGNWGLEKESQRITNSGDLALT
                     GHPAVFGNKLTNPHITTDFAESQIELITPPFRNIEDTYRALETLQAEVEAGIGPELLW
                     PLSMPPRLPAEEDIPIAVFPDTLEGREKAVYRRGLALRYGKKMQMISGIHYNFSFGPE
                     MMDYLFRVFGKGRSKRDFADELYFAVARNFFRYRWLLIYLFGASPSVDVSYDSVIANQ
                     LRAVAACCPGQFAESFHTMMDYPRYTTSLRVSSFGYAGPVPENGGISSYLKSLADYIK
                     VLRGLLGTKSQTHSKLGIYQKGVQMQLNDNVLQKESEFYSPIRPKQILNSGETQLEAI
                     EQRGVKYLEVRILDLNPFESLGINMEELYFLQVFMLFCLFETGGPLSKAEYDQANRNH
                     QLVSLLGRRPGLKLFDFRQGPILLQDWGRQLFFKLGQIADLLDKAVGAGQYQASVNAE
                     FQKINDISLLPSARIQREMEQGHLSFLEFGLHRAAVHRGESHRVMTQTV"
     gene            complement(4084..4392)
                     /locus_tag="VF531_00810"
     CDS             complement(4084..4392)
                     /locus_tag="VF531_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7712532.1"
                     /translation="METAELAKVFKALANEQRLHLFKLLYEWSGLEDSPESGYVSCDG
                     VEKCFTKACCSLDLSRSTISHHLKELQNSGLITFTRNGQCFVCKINTEVLNEIRNFLK
                     "
     gene            complement(4461..4925)
                     /locus_tag="VF531_00815"
     CDS             complement(4461..4925)
                     /locus_tag="VF531_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX7712533.1"
                     /translation="MGREIERKFLVNGTWYRDSSGCSLIKQGYLCNQTDRVVRVRVSG
                     SRGFITIKGKTAGLTRPEYEYEIPLPDAEEILKGLCGGTLIEKKRYLLEYQGFTWEVD
                     EFLGANQGLVVAEIELAEETTAFPKPEWIGAEVTGDPRYLNANLIKKPYLKW"
     gene            complement(4994..5197)
                     /locus_tag="VF531_00820"
     CDS             complement(4994..5197)
                     /locus_tag="VF531_00820"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HEX7712534.1"
                     /translation="MLDKHSTRFDNYQMAATLEKEITLARRMMEKLWNERGYTDKAVL
                     DASIKVDTLLNRYEQLKENDIIY"
     gene            5475..>5868
                     /locus_tag="VF531_00825"
     CDS             5475..>5868
                     /locus_tag="VF531_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7712535.1"
                     /translation="MKIKMLIIEDEPEWIDLYSQELRLLDSIEITGAATTANRGIELA
                     KSEHPDIILMDLNLSNNDFDGINAIDEILQDIDTKIIVVTSHFDKALVEEAFYAGAVE
                     YLLKHNLERLAETIQEVYENTSPHMILAN"
BASE COUNT         1539 a         1464 c         1262 g         1603 t
ORIGIN      
        1 ttggatttgt tagcggatat ttcagcgaat gtttgccaaa agatccgtaa attcattgat
       61 tgtcaacttc ttaattggtt tgccggttta cccaattata tcaagcttta tttgcctaat
      121 ttgatctgcg aaagttgagt aaatatttcg tcgcaaaaaa aaaattccct ccatactgct
      181 attctgatat gaaacttact gttctggcgg tgatatcgaa atttcatcat tcacctgggc
      241 ggcgattcaa tccacgaaga aagcatcgac ttgcacgccc tactccattg actcttttgt
      301 tacgatgcat caaaacaata tcgatcttgc atttactttt gatcgtcatt ttaatgaaca
      361 gggttttgag gttttagggt taaagcttca gtaaagccat acagttctca tcctgatttg
      421 acttttcccc ctttcacttt tccagtgcct cacacctctt gcctacgcct atctcaataa
      481 agcaaattta gattaacttc tctgctgttt tcatagaatt gcatgcgcct ttgccaatcg
      541 aaagaaaaat gaaggccaac ctcccctaac ccgagcgctt cggaaagcgc gacctcgagg
      601 aggggaattt taagaaagat taccgggggc accagagcgt ccgttccgga cgctagtccc
      661 cggcagggcg cgccttctac ggaaggcgac ctcgccaaca aatttggcgc gcaccctttc
      721 aaaaccacag ttttacgagc tttctgcctc tcgtgcctat tgcctgttgc ctgatctaat
      781 actcacgcct catgcctcac gcctattgcc tgttgccttt cgccgcaccc gggcgctaca
      841 atttttactc caaaccgctt gataacctgt cccggattgc cgacaaccaa tgaatgagaa
      901 ggcacatctt tggtgacaat gctgcccgcg ccgatttgtg aatgatcccc cacggtgatt
      961 ggtcctaaaa tcattgcccc ggcgccgatg atcacctgat cgccgatggt gggatggcgt
     1021 ttgccggtcg cagcaacact cgcattgccg agggtgactt gatgaaacag aagcacatca
     1081 tgaccgatct cggcggtctc cccgatgact accccatggc cgtgatcgat aaaaaagccc
     1141 ctgccgatcc gggcaccggg atggatctcc actccggtca ggaaacggga aacctgggat
     1201 atgagcctgg gaatgaaggg taccttgatc tgataaagca gatgcgccat ccggtgcata
     1261 ctaatcgccc acagtcccgg atatgtaatc agttcgagca gggctaaagg tttgccccgc
     1321 agggccggat ctttatgata tgcgttgcga aaatcatctc ccagatatcg cagttcccga
     1381 aaggcacggc tgatcccggt ccaataagac ccggacttca tttgatccga atgatccatg
     1441 acaacctcct tgatatcccg gactaggctg tccggaacta atgctttgaa gggttatgca
     1501 gttcttaaaa ccccaacagg tcgaggacct tctgttccac acgccggttt tggccttgaa
     1561 aaggaaaatc atggatatgc aatgccggat taaaattaag ctcaatcaca cgataattcc
     1621 tttctgtcgg ttccgcggca agatcttgaa tgatgatatc cgccccgcag attttggcac
     1681 ctacggcacg ggccgccgct agagcgatag ttttgtagcc ttcgggaata atatcggtaa
     1741 agtcaaggct gtccccgccg gtgctgatgt tggagttttc ccgtaaatag acggttgcgt
     1801 cgttttgggg aatgaaatga aaatccctgc cctgtaactt aagataagca gcttcctccg
     1861 cacccaaatt gattttttcc aggggagtaa catacccttt gccccgtaac ggatcttggt
     1921 tcttttccgc tacgagttcc tcgatggtat gaattccatc tcccttaaca ttggcgggaa
     1981 cacgatgtaa aacggcgggt acttcatcgc caatcaccaa aaaacggtat tccttgccgg
     2041 gcacaaattc ttcgatcaag atctcatggt cgtattgaaa ggcctccttt acactatggc
     2101 aatagtcctc ccggctttga caggctttga tgatcttcac gcctttgccg aagttggtcg
     2161 atttcggctt gatgacaaac gcctttcccg aaaaactcgg atacacctgt tccgcttcgg
     2221 ccaccgaatg taccgttaca ccctccggta ccttgatccc gctttccctg aggatctgct
     2281 tggtaacttg tttgttttcc atgagtaacg gtgcgatata actgtcggca gaagttttgg
     2341 tggcctgctt gatgtattcg atatgggaat ctttccggag gcgaatcgta ttatcgtttt
     2401 tatcgagcac ttctacactg atcccgcgcc gtaacgcttc gtcaatcaag acccgggtgg
     2461 aaagctccat cccctcatac tcttcattca ttgataaacc ctccttatac cgtttgtgtc
     2521 attacacgat gagattctcc ccgatgaacg gccgctctgt gcaggccgaa ttccaaaaaa
     2581 ctcaggtgtc cctgttccat ctccctttga atccgggccg agggaagcaa ggaaatatca
     2641 tttatcttct gaaattccgc attgacgctg gcctgatatt gaccggcacc tacggcttta
     2701 tccagcaaat ccgcgatctg cccgagctta aaaaacagtt gcctgcccca gtcctgcaat
     2761 aagatcggac cctgccggaa gtcaaacagt ttaagtcccg gccttcttcc caataaagat
     2821 accagttgat gattgcggtt ggcctgatca tactcagcct tggaaagggg cccgccagtt
     2881 tcaaagaggc agaagagcat aaatacttgc aggaaatata gttcttccat attgattcct
     2941 aaagattcaa aggggtttag gtccaggatc cgaacctcaa gatatttcac acctctttgt
     3001 tctatggctt ctaactgggt ttctccggaa ttcaggatct gcttcggacg gatgggagaa
     3061 taaaattcac tctctttttg cagaacatta tcattcaatt gcatctgaac gcctttttga
     3121 tagattccta gtttcgaatg ggtttgggac ttggtaccca ataaaccgcg taaaaccttg
     3181 atataatccg ccagactttt gagataagag gatatgcctc cgttctcagg gacgggaccg
     3241 gcatacccaa aactgcttac cctgagcgaa gtcgtatacc gtggataatc catcatggta
     3301 tgaaagcttt cggcaaactg ccccgggcaa caagccgcca cggcccttag ctggtttgca
     3361 attaccgaat cgtaacttac gtcgaccgaa ggtgaggctc caaaaaggta gatcaggagc
     3421 caacgatatc taaaaaaatt tctggccaca gcgaaataca gttcatctgc aaaatctctt
     3481 ttgctgcggc cttttccaaa aacccggaac aaatagtcca tcatctccgg accaaaggag
     3541 aagttataat gaataccgga gatcatctgc atttttttgc cataccgcag tgccagccca
     3601 cggcggtaga ccgctttttc cctgccctcc aaggtgtccg gaaatacggc aatcggaata
     3661 tcctcttcag cggggagccg cggcggcatg ctgagcggcc ataaaagctc cgggccgatt
     3721 cccgcttcca cctccgcttg aagagtttct aaagcgcgat atgtatcttc aatattgcgg
     3781 aacggcggcg tgatcaactc gatctgactc tccgcgaagt cggtcgtgat atggggattg
     3841 gtaagcttat tgccaaatac ggcgggatga ccggttagcg ccaagtcccc ggagtttgtt
     3901 atccgttgtg attctttctc caggccccaa ttaccgtcca gcagtaaccg cgcttgatcc
     3961 ggaccgctaa atgaggcgag gtttttagaa aagttccggt tcatgggttc cacctcacag
     4021 aatcattcta gagttcgaca atcaaagagt ttctaaacaa tcgaacaatc gcgataaaaa
     4081 aatttacttt aagaaattcc gaatctcatt caacacttcg gtattgattt tacaaacaaa
     4141 acattggcca ttccgggtaa aggtaatgag tcccgaattt tggagttcct ttaaatgatg
     4201 agatatggtg gaacgggata aatccaatga gcaacaagct ttggtgaagc acttctccac
     4261 gccgtcgcag gaaacatagc ccgattccgg ggaatcctcc aaaccgctcc attcatacaa
     4321 aagtttgaag aggtgtaaac gttgttcatt ggcaagagct ttaaaaacct tagctaattc
     4381 tgccgtttcc atagttctat aataatcgaa caatcaaccg atgtcaagta ttgaaatgaa
     4441 gaatgcgcct aacgcacgaa ttaccattta aggtaaggct tcttaatcaa attggcattc
     4501 aaatacctgg ggtcgcccgt tacctccgcg ccaatccact ccggcttggg aaaagcggtc
     4561 gtctcctccg ccagctcgat ctcggccaca accagccctt ggttggcgcc taaaaactca
     4621 tcgacttccc aggtaaagcc ctgatattca agcagataac gttttttttc aatcagggtc
     4681 ccgccgcata aaccttttag gatctcttcc gcgtctggca acgggatctc atactcatac
     4741 tcaggtcgcg tgagacccgc tgttttgccc ttgatggtga taaatccccg actgccgctt
     4801 accctgaccc ggactaccct gtcggtttga ttacacagat aaccctgttt gatcaggctg
     4861 catccggatg aatcccggta ccacgttccg ttcaccagaa atttccgctc gatttcccgg
     4921 cccacttgat tccccccgcg tttcaaatac cttatgatga agctgttctt attgatcaat
     4981 aaggtaccga caatcaataa attatatcat tttctttgag ttgttcatac ctgttcagca
     5041 aagtatcaac ctttatgctg gcatctaaaa ctgccttatc agtgtatccc cgttcattcc
     5101 aaagtttttc catcatccgc cgggccaagg tgatctcttt ttccagggta gctgccatct
     5161 gataattatc aaatcgggta ctatgtttgt caagcatctt atgtctccca ttcggcaaag
     5221 tacttaccat cctatgattg gctgataaaa ttatacaacg atatacaaat tacgggggtc
     5281 cgatattttt ttggtacctc tcgccgaatc ctcggatacc attctttacc tggagatggg
     5341 tgctatacca agataatacc tgaaatttta aaggattaat tggaataaac tagaattata
     5401 taaatgtaat cttatattat gaatgttcga aatagcgcgt gattaatttt ttatttgatt
     5461 atgaaggtat ttaaatgaaa ataaaaatgc tgattatcga agatgaaccg gaatggatcg
     5521 acctttactc acaagagttg cgtttattgg acagtatcga aatcaccggg gctgctacta
     5581 cagctaacag agggattgag ctggcaaaaa gtgagcaccc ggatatcata ttgatggatt
     5641 tgaatttatc aaataatgat tttgatggga taaatgccat agacgaaatt ttacaggata
     5701 ttgatacgaa aataattgtg gtaacttccc attttgacaa agcattagtt gaagaggcct
     5761 tttatgccgg agctgtcgaa taccttctaa aacataatct ggaacgtttg gcggaaacga
     5821 ttcaagaggt ttacgaaaac acaagcccgc atatgatatt ggccaaca
//
LOCUS       DASVMB010000025         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_77109,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000025 DASVMB010000000
VERSION     DASVMB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77109"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF531_00830"
     CDS             <1..422
                     /locus_tag="VF531_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7712536.1"
                     /translation="DAIPAKHLEFDRTSDGSYPYLGFIIHLNGVTIYHAGDTIIYEGL
                     AETLAQNQIDIALLPINGGDPDRLRRGFISNMKFKEAADLGAASGARLLIPMHYGMFP
                     VNDEKVENFVMFLQNTYPRQSFRVMELGKRFDFSKAS"
     gene            568..1233
                     /locus_tag="VF531_00835"
     CDS             568..1233
                     /locus_tag="VF531_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7712537.1"
                     /translation="MTYFRGSKEVVVLINLYIVDDHPFVREGLKTYLGAQKEIAIVGE
                     AAEGETALTEMLRLQPDVAVVDLHLPGMSGVALTKAVKEQGLFTRIIILSSFCEDEEV
                     MAAIEAGALSYLMKDSPPPKLLEAIMAARDGEPVLHPRIAKKLMQRVSRPQPMVEPLT
                     QKEREVLGLLVKGNSNKEIARNLSISETTVKTHVSNILQKLEVSDRTQAVIKAIETRL
                     LGG"
     gene            1237..2295
                     /locus_tag="VF531_00840"
     CDS             1237..2295
                     /locus_tag="VF531_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7712538.1"
                     /translation="MKKLMIDVLRDSLKGALAGIAISLTFLFTVSQSSLTPIFLIQAV
                     LFGITIGILISFVCSLFARTLSILPARFQRWLIFPDIIFYYLMSVAIFYEVTVNFGIF
                     FSLTDFSRDEIFYISLGVGVVSVMTGLLLANASQIEERLRLEKSCRELAVLEERNRIA
                     RELHDSVSQNLFGINLTLSSLDSLLEHDPEQVRRVIGHLKEMVGDVQTEMRLMIYGLR
                     PPALAEKGFFEAVESLANLYKTRYNLNLVCQIRGSDERISEKTQLALYRILQEALNNI
                     VKHSRATKAAVELKRNAAELVLTVRDNGGGFDTASVQSGHLGLEMMRERARQNQGEFS
                     ISSVPGSGTEISIRIHTD"
     gene            2524..3180
                     /locus_tag="VF531_00845"
     CDS             2524..3180
                     /locus_tag="VF531_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7712539.1"
                     /translation="MTGFLLGLSTGTVCLGYCSPILVPYLLGRGGDLRRNFLSVARFC
                     AGRLFGYLLFGFLAWLVHGFFTGNNGFRMVLFGLADIILAVLLMFFGFTATKSFCAAE
                     KASGFLSRIGMGLAPVHQLDFVAWMPLVFGFLTGINLCPPFLLAFTEAMATKSLAGSV
                     GYFFMFFLGTTLYLLPLPFLGVMRQKTVFATVGKYTAVIAGVFYVYQGIVMLIGGILG
                     "
BASE COUNT          731 a          714 c          869 g          870 t
ORIGIN      
        1 tggacgccat accggccaag cacctcgagt tcgatcgtac cagtgacggc agttatcctt
       61 acttgggatt catcattcat ctgaacgggg ttacgattta ccatgctgga gataccatca
      121 tctatgaagg actggctgaa acattggctc agaatcagat tgatatcgct cttcttccga
      181 tcaacggtgg tgatccggac cggctgcggc gcggattcat atccaatatg aaattcaagg
      241 aagccgcgga tctgggggcg gctagcggcg ccaggctctt gattccgatg cattacggca
      301 tgttcccggt gaacgacgag aaggtagaga actttgtgat gtttctgcag aacacatatc
      361 cccggcaatc gtttcgagtc atggaacttg gtaagagatt tgatttttca aaagcaagct
      421 gagattaagt ctttacgact cctccgaaag taggagtgga actcctcctt tttgtcttac
      481 gccggccccg ccaagggccg atccttgatg gacagttttt ttgtataatg gatcttgact
      541 ccagaggctt tttcccggaa aaccgaaatg acgtatttta ggggcagcaa agaggtggta
      601 gtcctgatta atctatatat cgttgacgat catccttttg ttcgggaggg gctcaagacc
      661 tatctcggcg cacagaagga aatcgcgatc gtgggtgaag ccgcggaagg agagacagcc
      721 ctcaccgaga tgctacggct ccaaccggat gtggcagtgg tggatctgca tcttccgggg
      781 atgagcggtg tcgcgttaac caaggccgtg aaagagcagg gacttttcac ccggatcatc
      841 atcctctcta gtttctgtga agatgaggaa gtcatggcgg cgattgaagc cggggcgctc
      901 agttatttga tgaaggactc gccgccccca aaattactgg aagcaatcat ggcggcccgg
      961 gatggggaac cggtattgca tccgcggatt gccaaaaaac tgatgcaacg ggtgagccga
     1021 ccccagccca tggttgaacc gttaacccag aaggaacggg aggtgctggg gttattggtt
     1081 aagggcaact ccaacaagga gattgccaga aatctttcga tctcggaaac gactgtgaag
     1141 actcatgtca gcaatatcct gcaaaaattg gaagtcagtg atcggacgca ggcagtcatc
     1201 aaggccatcg agaccagatt gctaggcggg tgaacgatga aaaaattaat gattgatgta
     1261 ttgcgggatt cattgaaggg tgctctggcc ggaattgcga tctccctaac cttcctattc
     1321 acagtctcgc agtcttcatt gactccgatc tttttgatac aggcggtgtt gttcggaatc
     1381 accattggta tcttgatctc ctttgtctgt tcattgtttg ccagaaccct aagtatactg
     1441 ccggctaggt ttcaaaggtg gttgatcttc ccggacataa tcttctatta tctgatgagt
     1501 gtcgccatct tttatgaagt taccgtaaat ttcgggatct ttttttctct gactgatttc
     1561 tctcgtgatg aaatcttcta tatcagccta ggagtagggg ttgtttcggt gatgaccggt
     1621 ttgttattgg ctaatgccag tcagatcgag gagcggctcc ggcttgaaaa gagctgtcgg
     1681 gaactggccg tgctcgagga acggaaccgg atcgcccggg aactgcacga ctcggtctct
     1741 caaaaccttt tcggaatcaa cctgaccctg agttcactcg attccctttt ggagcatgat
     1801 ccggagcagg tccgccgggt catcggccat ttgaaggaga tggtcgggga cgttcagacg
     1861 gagatgcgct tgatgatcta cgggctccgg ccaccggcct tggcggagaa gggttttttc
     1921 gaggcggttg aaagcctggc caatttatat aagactcgct acaacttgaa tctggtctgc
     1981 cagattcgcg gctctgacga gcggatttcc gaaaaaactc aattggcttt gtatcggata
     2041 cttcaagaag ccctaaacaa tatcgtcaaa cattcccggg cgaccaaggc cgctgtggaa
     2101 ttgaaacgga acgccgcaga actcgtgtta accgtcaggg acaacggggg cggtttcgac
     2161 acggcttctg tccaatcagg tcatctgggg ttggaaatga tgcgggaacg ggcacggcag
     2221 aatcaagggg aattctcgat cagctccgta cccgggtcgg gaacagagat cagtattcgg
     2281 atccataccg attgattcaa ggttcctttt ttgataaagt cagtcattcc ttcgaaggta
     2341 ggagagaaat ctcctacctt ttttgttttc aaatcagttc caaggctgat gccggaaact
     2401 gtggtgtttt gtacaatgaa agaaactgag tttgtggtaa aaacggcgaa tctccgggaa
     2461 atgtcgtgga tttacggata ctcgcggata atcattgatg tataaaggag cgttgcaagg
     2521 aaaatgaccg gctttctttt gggtttgtcc accggaacgg tatgccttgg ctactgcagt
     2581 ccaatcctcg ttccctatct gttggggaga ggaggtgatc tgcgccggaa tttcttgtcg
     2641 gtcgcccggt tttgcgccgg ccgtttattc ggttacctgc tattcggttt tcttgcctgg
     2701 ctggttcatg ggttcttcac cggtaacaac ggtttcagga tggttctttt cggtttagcc
     2761 gatattattc tcgccgtctt gttgatgttt ttcgggttca ccgcaaccaa atcattttgt
     2821 gcggcagaaa aggcgtcagg ctttttatcg cgcatcggaa tgggcttagc ccctgtacat
     2881 cagttggact ttgtggcctg gatgccactg gttttcggct ttttaaccgg gattaatcta
     2941 tgtcccccat ttttattggc atttacggag gcgatggcca ccaaaagcct ggcagggagt
     3001 gtgggctatt ttttcatgtt tttcctgggt acgaccttat atttgctccc tttgccgttc
     3061 ctgggggtta tgagacaaaa aacggtcttt gcaacagtag gtaaatatac ggcggtgatt
     3121 gccggtgtct tttatgttta ccagggtatc gtcatgttga tcggaggtat tttaggatga
     3181 gaag
//
LOCUS       DASVMB010000026         6931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_2904,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000026 DASVMB010000000
VERSION     DASVMB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2904"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2461)
                     /locus_tag="VF531_00850"
     CDS             complement(95..2461)
                     /locus_tag="VF531_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX7712540.1"
                     /translation="MNQGKYGYFDIENREYVITDPQTPTPWINYLGDGGYGGIISNTG
                     GGYSFDSDPRAKRVLRYRYNAIPEDQPGRYIYLRDDDSGKFWSATWQPVKAEYDMYQC
                     RHGLGYTEILQNNQGISSHIRYFVPRGKKWEIWRLKLHNPSDRPRQLSVFTYAEFSFF
                     DAVKDQQNVDWVQQIGQGEYEANFIFWNAFMKTWDYTFMATSLPPDSFDTSRDSFVGR
                     YHDLNRPLAVESGRCSGSIAHRGNGVGAFCHRIHLAPGAATELIYCLGVTPSKEAIRS
                     EVEPLLNSHTIQELFTELKGNWRNYLDSFQVETPDPDLNLMLNTWNPYQCKTTFNWSR
                     FVSLYQLGVDRGMGLRDSAQDILGVLHAVPEQAKQLLIMLCQCQFADGHAYHLFYPLT
                     GEGSMGEAQGGKYNWYSDDHLWLIEATVAYLKETGSFELLQESITYAEKDRQGTIWEH
                     LVRSIDFTCGHLGEHGIPLNGFADWNDALNLDRGKGKAESVWTGMLFCRELGLMAELA
                     AYLGKNSDAAKFEQLRDQTKEAINRHAWDGEWYLQAFDDDGMKVGSHASPNEEQIYLN
                     PQSWAVLSGVAPGERARLALTKAEEFLGTDMGLVLLFPAYKAFRSDRGGVTTYPPGAK
                     ENAGIFVHTNPWFIIAQMLVGENDSAYNNYRKILPVLKNAIPDRHQVEPYVYCQNILG
                     KEHPGFGMGRNSWLTGTAAWAYLAGTQYLLGIRPQYDGLMVDPHLPKGWSECTVWRRF
                     RGAELEIRYTLSQQSACRWYLDGEPLEKPGFIPIERLKNGGRHQIEVK"
     gene            complement(2515..3534)
                     /locus_tag="VF531_00855"
     CDS             complement(2515..3534)
                     /locus_tag="VF531_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7712541.1"
                     /translation="MPSTLYDVAKLAGVSPKTVSRVLNESHLVSGTTRKRVDDAIREL
                     DYHPNAIATSLKKQRSNIIGFMVPYGSDFVFQDPNMMEQLRGVHDLLTQEGYEVIVSA
                     PVYKKEAFTETLRLVKHRNVDGVILYPSAGIEQIIHEFKVKNFHYVTLGTCYSEQKHN
                     FVELDVTPGAYMAAKHLISLGHREIGLINKPSSFFIYGKDDLLTGYRTALEEQKLPFQ
                     SRLVREGDYTFSGGYQAFQILYDFNPKLTAVICASDPMTYGVIKAIQDMGLQVGKDIA
                     VVAGDNLPLTQKLFPDISCINNPAYQQGSLAGKILTTVIREGHDIPGVTLKTDFTVRG
                     QVPRF"
     gene            complement(3546..5975)
                     /locus_tag="VF531_00860"
     CDS             complement(3546..5975)
                     /locus_tag="VF531_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX7712542.1"
                     /translation="MKFGYFDDQSQEYVITTPRTPSPWINYLGTQRMFGLISNTGGGY
                     CFNTDARLRRITRYRYNNIPADSGGRYFYINDAGTIWSPTWQPVQTELDHYECRHGLG
                     YTRISGEKNQLAVEVLYFIPLDKDCELHRVKITNRSDQPKKFQFFSLIEFCFWNAYDD
                     MTNFQRNFSTAEVEVEGGTIYHKTEYRERRNHYAFYSVNHPISGFDTDRESFIGQYNG
                     FHNPQVVVAGRSSNSVADGWTPVASHHLEINLAPGASQELIFVLGYIENELEAKWEKP
                     GIINKAKARAMSEAFSRTDQVDRAFAELQSYWQQVLGVYQIQGGDERLGRMVNTWNPY
                     QCMVTFNLSRSASYFESGIGRGIGFRDSNQDLLGFVSLIPERAHGRILDLAATQFPDG
                     SAYHQYQPLTKKGNSDIGSGFNDDPLWLIYAVIAYIKETGDFSILDEAVPFNNDPANT
                     ASLMEHLRRSLSHVTANLGPHGLPLIGRADWNDCLNLNCYSNTPDESFQTCTNKDGKT
                     AESVFIAAMFVDIAPEYAALCRRLHLEREAETVLAAADQLRQAVMKHGFDGEWFLRAY
                     DDRGAKVGSHENEEGQIFIEPQGFCVMAGIGIAEGYAQKALDSVKKRLDTAHGVRLVN
                     PAYASYHLELGEISSYPPGYKENAGIFCHNNPWIMIAETILNRPDRAFEYYKKIAPAY
                     REELSDLHRMEPYVYSQMIAGCDAKNHGQAKNSWLTGTAAWNYLAITQYILGIRPDFD
                     GLRINPCLPAEIGEVEITRRYRGVTYRIRIENRRSGNYRLTVAGKEIPDHDNLIPYRS
                     DEKTVDVYCET"
     gene            6277..>6931
                     /locus_tag="VF531_00865"
     CDS             6277..>6931
                     /locus_tag="VF531_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712543.1"
                     /translation="MLKNETVFTGTVGRKKVILRGILHQKPRDKLTATLWRFVRCNEG
                     QNGTRPSKMIMAKGGMTVLFLAIFFLVFALFIVARVFRGNQAIWLGLVLFTFGCCILG
                     LVGLVPRFGNYRLDGLLSLPMDEPGWAWAVLARLSLYNFIRFRLWSAVGFLVAFIGFA
                     ISYSRERLRTGDRVIIGAVVFGAALMVWNYDPGHLFEVYKRGAILVARLNERRYWELS
                     "
BASE COUNT         1602 a         1721 c         1876 g         1732 t
ORIGIN      
        1 acacaatttg accctgccgt tgtcgattgt ttcattcaat tgttattgga ttatgaaaat
       61 cttcagaatg aggtggctgc gggtacgata acgctcactt gacctcgatc tggtgccgtc
      121 ccccgttttt aaggcgttct atcggaatga aacccggttt ttccaagggt tctccatcca
      181 agtaccagcg gcaggcggac tgttgggaaa gagtgtaccg tatctcaagc tcagcaccac
      241 ggaaacgccg ccaaaccgtg cattccgacc aacctttcgg caggtgcggg tctaccatca
      301 gaccatcgta ttgggggcgg attcccaaga ggtactgggt accagccaga taggcccagg
      361 ccgcggtacc ggtcagccat gaatttcttc ccataccgaa cccgggatgt tctttaccca
      421 agatattttg gcaatagacg tacggttcga cctgatgccg gtcgggaatg gcgttcttca
      481 ataccggcag aatcttacgg tagttattgt aggccgagtc attctctccc acaagcatct
      541 gggcgatgat gaaccagggg ttggtatgca cgaagatgcc ggcgttttcc ttggccccgg
      601 gagggtaggt ggtgactcct ccccggtcgg aacggaacgc cttataagcc gggaatagca
      661 gaaccaatcc catgtcggtt cccagaaatt cctccgcctt tgtcagggcg agtcgcgccc
      721 tttccccggg ggccacaccg gaaagaaccg cccaggattg cgggttaagg taaatctgtt
      781 cttcattcgg agaggcatgg gagcctactt tcattccatc atcgtcaaag gcttggagat
      841 accactctcc atcccaggcg tggcggttga tggcttcctt ggtttggtcc cgtaattgtt
      901 caaattttgc cgcgtcggag ttctttccca gataagcggc gagttccgcc atgagcccca
      961 gttccctgca gaagagcatc ccggtccaga ccgattcggc cttgcctttg cctcgatcca
     1021 gatttagggc gtcgttccaa tcggcaaagc cgttcagggg aatgccgtgc tccccgagat
     1081 ggccgcaggt gaaatcgatc gagcggacca ggtgctccca gatggtccct tgccgatcct
     1141 tctcggcgta tgtgatagac tcctgcaaca actcaaagga accggtctcc ttaaggtaag
     1201 cgacggtggc ctcgatcagc cagaggtgat cgtcggagta ccaattgtac ttgccgccct
     1261 gggcctctcc catgctgccc tcgccggtca gcggataaaa aaggtgatag gcatgaccgt
     1321 ccgcgaattg gcattgacaa agcataatca aaagctgttt cgcctgttcc ggtacggcgt
     1381 gcaacacacc caggatatcc tgggcgctgt cccgcaatcc catgccccga tcgactccca
     1441 gttggtacaa tgagacaaag cgcgaccagt taaaagtggt tttgcattga taggggttcc
     1501 atgtattcag catcaggttg agatccggat ccggggtctc tacctggaaa gagtccaaat
     1561 agttccgcca gtttcccttt aattccgtga aaagctcctg aatggtatgg gaattaagga
     1621 gcggctccac ctccgaccgg atggcttcct tggatggagt aacccccaga caatagatga
     1681 gttcagtggc ggcacccggg gccaagtgga tccggtggca gaaagctccc actccgttgc
     1741 cgcggtgggc gatgctgcct gaacagcgcc cgctttcaac tgccaacggc cggttcagat
     1801 cgtgatagcg tccgacgaaa ctgtcgcggc tggtatcaaa ggagtcgggt ggcaggcttg
     1861 tggccatgaa agtgtaatcc caagtcttca tgaaggcatt ccaaaagatg aagttggcct
     1921 cgtactctcc ctggccgatc tgttgtaccc agtccacgtt ctgttggtct ttcacagcat
     1981 caaagaagga aaactcggca taggtaaaga cactgagctg ccgggggcga tcgcttgggt
     2041 tatgaagctt gagccgccat atctcccact ttttgccccg gggaacgaag taacggatgt
     2101 gacttgagat gccttgattg ttttggagta tttcagtata gcccagccca tggcggcatt
     2161 gatacatatc atattcagcc ttcaccggtt gccaggtggc cgaccaaaat ttaccggaat
     2221 catcgtcgcg taaatagatg taccgtcccg gctgatcctc gggaatggcg ttgtaacggt
     2281 atcgtaagac acgcttcgcg cgggggtcgg aatcgaaact atatccgcct ccggtattgg
     2341 agatgatccc gccgtaaccg ccgtccccca aatagttgat ccacggcgta ggggtttggg
     2401 gatcggtgat gacgtactcc cggttctcga tgtcgaaata gccgtatttc ccttggttca
     2461 tcgttatcaa cctcgcttga tcaaaaatag ggaatctcca cgtatgcttt ggggttaaaa
     2521 ccgggggact tggccccgga ccgtaaaatc ggtttttagc gtgacccctg gaatatcgtg
     2581 gccctcccgg atgactgtag tgaggatttt cccggccaga ctgccctgtt ggtaggcggg
     2641 attgttgata cagctgatat ccgggaataa cttctgggtt aatggcagat tatcaccggc
     2701 aacgaccgcg atatccttgc caacctgaag ccccatatcc tgaatggcct tgatcacgcc
     2761 atacgtcatc ggatcgctgg cacagatcac cgcggtgagc ttggggttga agtcatagag
     2821 gatttgaaag gcttgataac ctccactaaa ggtataatcc ccttcccgga ctaaccgaga
     2881 ctggaatgga agtttctgtt cctcgagtgc ggtgcggtaa ccggtcaata gatcatcttt
     2941 gccgtaaata aagaaactgc tgggcttgtt gattaggccg atctcgcggt gacccaatga
     3001 gatcaaatgt ttggcggcca tataggcgcc gggggttaca tcaagttcta caaagttgtg
     3061 cttctgttcg gagtaacagg ttcccagcgt tacataatga aaatttttca ccttaaactc
     3121 atgaatgatc tgttcaatcc cggccgaagg atagaggatc actccgtcga cattccggtg
     3181 tttcaccaag cgcagcgtct cggtaaaggc ttctttcttg tagaccgggg cggagacgat
     3241 cacttcatac ccttcctgtg tcagaagatc gtgaactccg cggagctgtt ccatcatatt
     3301 aggatcctga aagacaaagt ccgaaccgta agggaccatg aaaccgatga tgttggaacg
     3361 ctgtttcttt agactggtcg cgatggcatt gggatgatag tccaattccc gaatggcatc
     3421 atctacccgt ttccgggtgg taccggagac cagatggctc tcgttgagca cccgggaaac
     3481 ggttttggga gaaaccccgg ccagtttggc tacatcatag agggttgacg gcatttagat
     3541 tcgcctcaag tttcgcaata tacatcgaca gttttttcat cggaacgata tgggatcaga
     3601 ttatcatgat ccggaatttc cttaccggcg acggttaaac gataattgcc gctgcggcgg
     3661 ttctcaatcc ggatccggta ggtgacaccg cgatatctcc gggtgatctc gacttctccg
     3721 atctccgccg ggagacaggg attgatccgt aaaccatcga agtcaggcct gattcccaaa
     3781 atatactgag tgatcgccag atagttccag gcagctgtcc cggtcagcca ggagtttttg
     3841 gcttggccgt ggtttttggc atcgcagccg gcgatcattt gcgagtaaac atagggctcc
     3901 attcggtgca ggtcactgag ctcctcgcgg taggccgggg caatcttctt ataatattcg
     3961 aaagcccgat ccggtcggtt gagaatggtc tcggcgatca tgatccaggg attgttatga
     4021 cagaagatcc cggcgttctc cttgtatccc ggcgggtaag aactgatctc gcccaactct
     4081 agatggtagg aggcataggc cggattgacc agccggacgc catgggcggt atcaagacgc
     4141 tttttcaccg agtccagggc tttttgggcg taaccttctg caatcccgat ccccgccatc
     4201 acacaaaatc cctggggttc aatgaagatt tggccttcct cgttttcatg gcttcccact
     4261 ttggcgccgc gatcatcata tgcccggaga aaccattcgc catcgaagcc gtgtttcatt
     4321 accgcctgac gtaattggtc cgctgcggct aaaaccgtct cggcctccct ctccagatgg
     4381 agacgacggc agagtgcggc atattccggg gcaatatcca caaacatggc cgcgataaat
     4441 acggattcgg cggttttccc gtccttattg gtacaggtct ggaaggactc atccggtgtg
     4501 ttcgagtagc agttaagatt gagacaatca ttccaatcgg cccggccgat caggggtaaa
     4561 ccgtggggac ccaaattggc tgtaacatga ctcagggaac ggcggagatg ctccattaag
     4621 gaggcggtat tcgccgggtc attgttgaac ggcaccgctt catcgagaat ggaaaaatcg
     4681 ccggtctctt taatataggc gataaccgca tagatcagcc atagcgggtc atcgttaaaa
     4741 ccgctgccga tatcggagtt accctttttg gtaaggggtt gatattgatg ataggcactg
     4801 ccgtccggga attgagtggc ggccaaatcc aaaatccggc cgtgggcgcg ttccggtatc
     4861 aaggaaacga aaccaagcaa atcttgattg gaatcacgaa aaccgattcc cctgccgatt
     4921 ccggattcga aataagaggc gcttcgggaa agattaaatg taaccataca ttgatagggg
     4981 ttccaggtgt tcaccatccg gcccagccgt tcatcgccgc cttgaatctg ataaaccccg
     5041 agtacttgtt gccaatagga ttgcaattcg gcaaaggccc ggtcaacttg atcggtacgg
     5101 ctaaaggcct ccgacatggc acgggctttg gctttgttaa tgattccggg tttctcccac
     5161 ttggcttcca gctcattttc gatgtaaccc aaaacgaaga tcagttcttg tgaagctccg
     5221 ggggccaagt tgatctccag atgatgcgag gccacaggag tccatccgtc cgccactgaa
     5281 ttggaggaac ggccggcaac gaccacctgg gggttatgaa agccgttata ctgaccgata
     5341 aatgattccc gatccgtgtc aaatccggaa atcggatggt taacactgta aaatgcgtaa
     5401 tggttacggc gttcgcggta ctcggtttta tggtaaatag tgccgccttc aacctccact
     5461 tcggcggtgc tgaagttccg ttggaagttg gtcatgtcat cataagcgtt ccagaaacag
     5521 aattcgatca atgaaaagaa ttggaacttt ttgggctgat cactccgatt ggtgatcttg
     5581 acccggtgaa gctcacaatc cttatcgagg ggaataaagt aaagaacctc tactgccaac
     5641 tggttctttt cgccggatat ccgggtataa cccagaccat gacggcactc ataatgatcc
     5701 agctcagttt ggacaggctg ccaggttggc gaccagatgg taccggcgtc attaatatag
     5761 aaataacggc cgccgctgtc ggctggaata ttgttgtaac ggtaacgggt gatacggcgc
     5821 aagcgggcat cggtattaaa gcaataaccg cctccggtat tggagattaa tccgaacatt
     5881 cgctgcgtcc ccaggtagtt gatccacgga gaaggagtgc ggggcgtagt gattacgtac
     5941 tcttgggatt ggtcgtcaaa ataaccgaat ttcatcgtaa tcagctcctc gttaaagacg
     6001 gctcaaattt gagatccgga aaagattgga tcgatttgtc tatcgttgtc agtataatcg
     6061 aaaggacaaa cccctgtcaa ggtggaaaaa atctagttgc cctctgatgc gggggcaaac
     6121 ggaaaataaa agcggggtga gaactcatga gtacctatga tgaaagtgtg tttttcgaaa
     6181 aggatttttt ggcttaggtt taatggcaaa gggtaatttt ttaagggtag taatgtcagc
     6241 ggtgtcagta agtttttcaa atctggagtt gaccttgtgc tcaagaatga aacagtattc
     6301 accgggacag tcggcaggaa aaaggtgatt ctccggggaa ttctacacca gaaacccaga
     6361 gataaattga ccgccaccct atggcggttc gtccggtgca acgagggaca aaatggaacc
     6421 cggccgtcaa agatgatcat ggccaaagga ggaatgacgg tgcttttttt ggcgatattc
     6481 tttttggtct ttgcattatt catcgtcgcc agggtgtttc ggggcaacca ggccatctgg
     6541 ctcggcttgg ttctatttac ttttggttgc tgtattttgg gtctggttgg cctggtaccc
     6601 cggtttggga attaccggtt ggatggatta ctgagcctgc ccatggatga gccgggctgg
     6661 gcttgggctg ttcttgccag gctttcactt tacaatttca tccgctttcg gctctggagc
     6721 gccgtgggat ttctggttgc atttatcggt tttgcgattt cgtactcgcg ggaaaggtta
     6781 cgaaccggtg atcgtgtgat tatcggagcg gtcgtttttg gtgcggcttt gatggtctgg
     6841 aattatgatc cgggacatct ctttgaggtc tataaacgcg gcgccatatt ggttgcccga
     6901 ttgaatgaac gccgttattg ggaattatcc c
//
LOCUS       DASVMB010000027         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_297349,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000027 DASVMB010000000
VERSION     DASVMB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297349"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF531_00870"
     CDS             complement(<1..222)
                     /locus_tag="VF531_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712544.1"
                     /translation="MIQVIPFRAEKEPLFLPLLARGLSDLSVLRLNALSIQAQVNVYL
                     ELDIRKFQSDQPVAEFNWNGQELWFTGMLQ"
     gene            complement(650..1225)
                     /locus_tag="VF531_00875"
     CDS             complement(650..1225)
                     /locus_tag="VF531_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712545.1"
                     /translation="MAKPKAASRNQPFFDKDRYWNGVFTGVFIGSFFLSLLLLIFIRV
                     QGFKLAINPEQLARLVQAKVESQARQDIPQLFQGFKQELPGEVDKHLGGLDDLTIGFG
                     QSQVKLPDEVLATIKLEFNRIIEESIANTLNDYDTSKYQERIGKSAYELVNQMLMREL
                     IGKTYLIKTSQWFSVPVKIVISNSDQLQIGI"
     gene            complement(1449..1907)
                     /locus_tag="VF531_00880"
     CDS             complement(1449..1907)
                     /locus_tag="VF531_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712546.1"
                     /translation="MLLFSLGMCALCFGIYGLVHYRRKVWGVIGGLAVIAFSIWLMGA
                     SFDIKTNLIGIHPIEETKSRVILEFHFFKDKDTGIRYYESRGISQYTLAAKYDGDTKL
                     FYQQLGKGKSAFRIIFPSEEKKTAIEVYSDFLLKNEIIPVYRPEKKQGKK"
     gene            complement(2216..3001)
                     /gene="trpA"
                     /locus_tag="VF531_00885"
     CDS             complement(2216..3001)
                     /gene="trpA"
                     /locus_tag="VF531_00885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7712547.1"
                     /translation="MNRINAGFQRLRADGEKAFIPYIMGGDPDLEATVSLIERLDAAG
                     ADLIEIGVPFSDPLADGPVIQQAAVRALQNGVNLNRLFGAVAVASRRSKVPLVLMIYY
                     NMILARGVESFCAAAHEAGCAGLIVPDLPPDEAGQLVELADHYQLGLNFLVAPTSSGR
                     RIERAIAATTGFVYAVSLKGVTGVRNELAPDLPQLVQRIRSCTTKPVAVGFGIATPEQ
                     ARTVATFADGVIVGSAIVAAVASDPSLLAAEKLARDLRAAIRT"
     gene            complement(2998..4191)
                     /gene="trpB"
                     /locus_tag="VF531_00890"
     CDS             complement(2998..4191)
                     /gene="trpB"
                     /locus_tag="VF531_00890"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7712548.1"
                     /translation="MDLTDRDEWYFGNYGGRYVPETLMAALDELTESYRHYAADPEFL
                     AELQDLYSHYSGRPTPLYYAERFSKELGRGMVYLKREDLNHTGAHKINNALGQILLAR
                     RMGKKRIIAETGAGQHGVAAATVAAKFGLECVVYMGEVDIARQALNVFRMKMLGAEVR
                     PVVSGSRTLKDATNEAIRDWVAHVDETYYLIGSVVGPHPYPQMVRDFQSVIGRETRAQ
                     LLEMEKRLPDWVVACVGGGSNAMGIFHEFLSCPEVRLVGVEAAGKGLNTEEHAASLGR
                     GKPGVLHGALSYLLSDGDGQVREAYSISAGLDYPGVGPEHSFLRDNGRVDYWSVTDEE
                     ALEAFTFLTRVEGIIPALESAHAVAAGMKIARELLCGQILVINISGRGDKDIYSVAAA
                     LGVKV"
     gene            complement(4169..4828)
                     /locus_tag="VF531_00895"
     CDS             complement(4169..4828)
                     /locus_tag="VF531_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7712549.1"
                     /translation="MIPVKICGITRLEDALSCIRHGVTALGFIFAPSKRRVTPDVVRE
                     ICKALPPFIVRVGVFVDQDPWVIRDLLRDCCLDLAQLHGNEPVKDAEILEGRVIKAFH
                     AGDAALDPIWRDAPLRAVLIDSGKSGSGGSGIVFDWNRVEPFRTLGFPLILAGGLHPG
                     NVERAIRTVRPDGIDLAGGVEKAPGIKDEAMIGDLMTVLRRMESDFDIDSRGDGLDRQ
                     G"
BASE COUNT         1265 a         1443 c         1135 g         1126 t
ORIGIN      
        1 ctgtaacatc ccggtgaacc aaagctcctg gccgttccag ttgaactcgg ccaccggctg
       61 gtctgattga aatttccgga tatccaactc taggtagaca ttgacctgag cctgaattga
      121 caatgcattg agacgcaaca cggaaaggtc cgacaaacct ctggccaaga gtggcaggaa
      181 taacggctct ttctcggcac ggaaaggaat gacctgaatc actgtatcct ccggaaactc
      241 caatatcttg acaccaacgt taacacaacc ggaactgata gtcaagtagg gcctgggccg
      301 aatcccgagg taaaaagctg ggtgtcgcgc cccccggggc gcgcggcaat agacaacagg
      361 gaacagggaa caggcaacag gaaaagacac acccgggcgg caagttttat gaacgcaagt
      421 cgtaatatta aaaacgaagg tcgaaaggct atagggaata aatcatgact aaaagtaaaa
      481 gatgtagtgg gtccggtaaa agaccataaa aaaacgggct aatgcccgca tcaactcacg
      541 actatggcag accttaaaag gcgccagcac ttactatcgg agatgctctt gaaacaggta
      601 taatctattc ctgttcccag ttgcctattc cctgttgcct gttgcctgtt tatatcccaa
      661 tctgcaattg atcactattg gatataacaa tcttgacagg caccgagaac cattgtgatg
      721 ttttaatcaa ataggtttta ccgatgagtt cccgcatcag catctgattt acaagttcat
      781 atgcgctctt gccgattcgt tcctgatatt ttgaggtgtc ataatcattc agggtattgg
      841 cgatgctctc ctcaataatc cggttaaatt cgagcttaat ggttgccaaa acctcatccg
      901 gcaacttcac ttgggactgg ccgaaaccaa tcgtcagatc atccaaaccg ccaaggtgtt
      961 tatcgacctc gccaggcaac tcctgcttaa atccttgaaa taactgtgga atatcctgcc
     1021 gggcctggga ttccaccttt gcctgtacta aacgggccag ttgctcgggg ttaatcgcca
     1081 atttaaaacc ttgtacccgg ataaatatca acaacagtaa agagagaaag aaactcccga
     1141 taaatacccc ggtaaaaacc ccgttccaat accggtcttt atcaaaaaac ggctgattcc
     1201 gactggcggc tttgggtttc gccattccga ttccccccgt ccatcttcca tgccctgact
     1261 cccgatggtt taaaatacga agctgctttt tgtagattta tatatgcatc cattcgatta
     1321 aaagcaactt ggtattactt cccatccggg ttcgtctgtt tcaggcggaa atagacaacc
     1381 tcttttttca tagtatgcca attcatggag ggaatatgat tcatatgtac ataaatgtac
     1441 ataaaacctt attttttccc ctgttttttt tcaggtcgat ataccgggat gatctcgttt
     1501 tttaacaaaa aatcactgta tacctcaatc gcagtctttt tctcctcgga gggaaatatg
     1561 attctaaagg cggatttgcc cttgcccaac tgctgataga atagcttggt gtcaccgtca
     1621 tactttgcag ccagagtata ctggcttatt ccccgggatt cgtagtagcg gatcccggta
     1681 tccttatctt tgaagaaatg aaactcaagg atcacgcggg atttcgtctc ttcaatcggg
     1741 tgaattccaa tcaaattggt cttgatatcg aacgaggccc ccatcagcca aatgctaaag
     1801 gcgataacgg ccaaaccgcc aatcactccc caaaccttcc tgcggtaatg caccaatcca
     1861 taaattccga aacataatgc gcacattccg agactgaata agagcatttg aaatctcctt
     1921 ttttaggagg tttgaacttt tgacgagctg ttttgtcaaa cgaaattcga aatcgttcga
     1981 tgaaacggtc atagctcaaa cctcccgaat ctgatccaca cagaatcctt ttaaaaatta
     2041 aacataaaaa cgaaaatccc tccggaaaat ttcaagaggg atgttcaata atatgactta
     2101 actgttgact ctgttcggac cctcccccat cgctatcgct ttttgataac cttacggagg
     2161 agggcgcacg taccgtgtac gcgaggcgac tttgatccta cccaaagggt gagggtcagg
     2221 tccggatcgc agccctcaaa tcccgggcca acttttccgc cgcaagcagg ctggggtctg
     2281 aggccacagc ggcaacgata gcgctcccta caataacccc gtcggcaaat gtcgcaacag
     2341 tccgggcctg ttccggggtg gcaattccga aacccaccgc caccggtttg gtggtgcaag
     2401 aacgaatccg ctgcaccagt tggggtagat ccggcgccag ttcattccgt accccggtaa
     2461 ctcctttcaa agaaacggca tacacaaatc cggttgtagc cgctatggcc cgttcaatcc
     2521 gcctgccgga actggtaggt gccaccaaaa aattaagtcc cagttgataa tgatctgcca
     2581 gttcgacaag ttgaccggcc tcatccggcg ggagatcggg aacaatcagc ccggcgcaac
     2641 ccgcctcatg ggcggcggca cagaaacttt ccaccccccg ggccagtatc atattataat
     2701 agatcatcaa taccagcggg actttactcc ggcggctagc tacggcaacc gccccaaaaa
     2761 gccggttcag attgacaccg ttctgtaacg cccgaaccgc agcttgttga atcacaggtc
     2821 catccgccag gggatccgaa aaaggcaccc cgatctcaat cagatccgct cctgcggcat
     2881 caagccgttc aatcaagctt acggtagctt cgaggtccgg atctcctccc atgatatatg
     2941 gaataaaagc tttctcccca tccgcacgga gtctttgaaa accagcgtta atccggttca
     3001 cactttcacc cccaatgcag cggcgaccga gtaaatatcc ttatctcccc gaccggaaat
     3061 gttgatcacc agaatctgcc cgcaaagcag ctcccgggcg attttcattc cggctgccac
     3121 ggcgtgggcg ctctccagcg cagggatgat cccctcgaca cgggtcagga aggtgaaggc
     3181 ctcaagcgct tcctcatcgg tgaccgacca ataatcgacc cgtccattat ccctcaaaaa
     3241 actatgttcc gggccgaccc ccgggtaatc gagtcccgcc gagatcgaat acgcctcccg
     3301 aacctgtcca tcaccgtcac tcaacagata actcagggcg ccatgcagta ctcccggctt
     3361 gccccggccc agtgaagcgg catgctcctc cgtattcagc cctttcccgg cggcttcgac
     3421 gccgaccagc cgcacctccg gacaggagag aaactcgtga aaaatcccca tggcgttact
     3481 ccctccccct acgcaagcca ccacccagtc aggtagacgc ttttccatct ctagaagttg
     3541 agcccgggtt tcccggccga taaccgactg gaaatcccgg accatctgtg gatacgggtg
     3601 aggtcccacc accgacccga taagataata ggtctcatcc acatgggcca cccaatcccg
     3661 gatcgcttcg ttggttgcgt cctttagggt tcggctgcct gaaacaaccg gccgtacctc
     3721 ggcgccaagc attttcatcc ggaagacgtt caaggcttgg cgggcaatgt ccacctcacc
     3781 catatagacc acacattcca gtccgaattt ggctgcgacc gttgcagcgg ccacgccgtg
     3841 ctgacccgcg ccggtctcag cgatgatccg tttcttaccc atccttcggg caagcaggat
     3901 ctggcctaaa gcattattga tcttatgggc tccggtatgg ttcaaatcct cgcgcttaag
     3961 ataaaccata cccctgccca gttctttgct gaaacgctcg gcataataca ggggcgtcgg
     4021 tcggcccgaa taatgactgt ataaatcctg caattcggct aaaaactcag gatcggccgc
     4081 ataatgacgg tagctttctg ttagttcgtc aagagccgcc atgagtgtct ccggcacata
     4141 gcggccgcca tagtttccga aataccattc atccctgtct gtcaagtcca tccccccttg
     4201 aatcgatatc gaaatctgat tccattcgac gtagaaccgt catgagatca ccaatcatgg
     4261 cctcatcctt tattccgggg gctttttcca ccccgccggc caggtcaatc ccgtccggcc
     4321 ggacggtccg gatcgcccgc tcgacattgc cgggatgcaa tccgcccgcc aaaatcaaag
     4381 gaaaacccaa ggtacgaaag ggttcaaccc gattccagtc aaagacgatc cccgaacctc
     4441 cggagccgct ctttcccgag tcgatcaaca ccgcccggag cggcgcatct cgccatatcg
     4501 ggtcgagagc ggcatccccg gcgtggaatg ccttgatgac ccttccctca aggatttcgg
     4561 cgtctttcac aggctcatta ccgtgaagtt gggccaaatc cagacaacaa tcacgaagca
     4621 gatcccggat gacccatgga tcctgatcca caaaaactcc cacccgtaca ataaacggcg
     4681 gtagcgcttt acagatctcc cggactacgt ccggggtcac ccggcgcttg gagggggcga
     4741 atataaagcc cagggcggtc acaccgtggc ggatgcaact gagcgcgtct tcgagccggg
     4801 tgatgccgca gatcttgacg gggatcatga ctccagacct cctagaaact cccggatcac
     4861 ggctccggga tcggctgcgg tcaccaatgc ttctccaaca agtaccgcct ggatcccggc
     4921 atccatcagc cgtaagacct gggaacggtg atggatcccg ctctcagca
//
LOCUS       DASVMB010000028         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_150688,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000028 DASVMB010000000
VERSION     DASVMB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150688"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VF531_00900"
     CDS             <1..1293
                     /locus_tag="VF531_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712550.1"
                     /translation="NLAKAVANLEEAARLFSPDSMVEFTSAHNYLGHTALTLAELSNP
                     QENFSRAVKAFEGALQVFNLTEHPEEYARAQNGLADAALGLADYSDTAANLTKAIDAY
                     NEALKVFTVEQYAADHAAIQNHLGDAYKYLAELGNSEDLFAKAIQAYETALKLFDPEK
                     APLDNGRTLLKLGDLCRAFAKVRNGESNLSKAIKTYQEALKIFTKEIYPTDYAMTQGS
                     LGYVLVDMAEVRDKEHYLLKAAKAFEEALNILTVWDHPEESVAIESTLGRIHLALADY
                     QETADNLRQAFQAFDETLKYYTIDKNPVEYATSLNNIGVTYKAMAESLDSMLFDTIEE
                     FQQSKAEKLNNAITAFQEALKVFNSETYPMDYAMTQNNLGASYSLMAEVDDKDHCLDN
                     ATIAFQEALKIYTPEADPENNKKVKTNLDRIKQRFGKA"
     gene            complement(1361..1573)
                     /locus_tag="VF531_00905"
     CDS             complement(1361..1573)
                     /locus_tag="VF531_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712551.1"
                     /translation="MGVLEETKIIQAINRFEDTRLLLNEVGRNDPRFSKRFNEIIEEL
                     KKFESELAEIRDQMNIENLGKHARER"
     gene            complement(1779..2561)
                     /locus_tag="VF531_00910"
     CDS             complement(1779..2561)
                     /locus_tag="VF531_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712552.1"
                     /translation="MTGFTAENFMSGQAVISEAILGKPSWPNRPKPVPLWQRNQLSFF
                     VTTGAEPRAMELMNAGSISSREVRERNDQGAINRMRQQTDWAVNPARGERPESATVLA
                     VETDQSFSGETNPFELEPERPTDVAVINPDPVVSFKEHQESLFNSLSLLALKKRSIQA
                     SEYMILSGSHALTRLAGKNSIPNPHMVNTRNRPAAVGGFFDDDPSDQDAGEEEIVGFT
                     VPFSFQTSSGYHPETWFPVAARIGLYQPVPPKPFGERCDYLM"
     gene            complement(2709..2864)
                     /locus_tag="VF531_00915"
     CDS             complement(2709..2864)
                     /locus_tag="VF531_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712553.1"
                     /translation="MDADKLDLVHRFSLTLGPKIDKALIKNCLTALAILSDEETGSEL
                     KKLIAKL"
     gene            complement(2955..>3749)
                     /locus_tag="VF531_00920"
     CDS             complement(2955..>3749)
                     /locus_tag="VF531_00920"
                     /inference="COORDINATES: protein motif:HMM:NF020939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HEX7712554.1"
                     /translation="FGAQDVVVQKFLIEQSVYSPVRKANEYRKLQNMADYAHTSKCLR
                     RFILEYFGEPHAPETCGNCSVCNEGEQIDITTGAQKIFSCILRMHERYGTAMVAAVLK
                     GARNQKIRATKMDQLSTYGIMQDLSLEEIRDLLNLLVADGYLRLSAGKFPVVKLSPKA
                     VAVLKNQEPVFRRVPKRPTKATADDSRASAQLFQLLRQTRREIAASEMVPPYIIFSDS
                     TLSELARVKPTTAEMMLGVKGVGEAKLTKYGATFLKVIQDYLRMQK"
BASE COUNT          975 a          944 c          871 g          959 t
ORIGIN      
        1 aatttggcga aagccgtggc caatctggaa gaggccgccc ggttattctc accggactcc
       61 atggttgaat tcacttcagc ccataattat ctgggtcata ccgcactaac tcttgcggag
      121 ctatccaatc cgcaagaaaa tttctccagg gctgtcaagg ctttcgaagg ggcactgcaa
      181 gtatttaacc tgaccgagca tcccgaggaa tatgcccggg ctcaaaacgg acttgccgat
      241 gcagcgttgg ggctggctga ttattcggac actgccgcca atctgaccaa agcgatcgat
      301 gcttataatg aagctctcaa agtgttcaca gtggagcaat acgccgcaga tcatgccgcc
      361 atccaaaatc atctgggcga cgcctataaa tacctggctg aactgggaaa ttccgaggat
      421 cttttcgcca aggcaattca ggcatatgaa actgccttaa aattattcga tccggaaaaa
      481 gctcctctgg ataacggccg tacccttttg aaactgggcg atctttgcag ggcctttgcc
      541 aaggttcgta atggtgaaag caacctatcc aaggccatta agacctacca ggaggctttg
      601 aagatcttca ccaaagaaat ttaccctact gattacgcca tgacccaagg cagcctgggt
      661 tatgtcctgg tggacatggc tgaggttcgg gacaaggaac attatttact taaagccgcc
      721 aaagcctttg aggaagctct caacatcttg accgtttggg atcatccgga ggaatccgtg
      781 gccattgaaa gtaccttggg aagaatccat ttagccctgg ccgattatca ggaaacagcg
      841 gataacctga ggcaggcctt ccaagccttt gatgaaacgt tgaaatacta caccattgac
      901 aagaacccgg ttgagtatgc aacctccctc aataatatcg gagtcactta taaggcgatg
      961 gccgaatctt tggacagcat gttgtttgac accatcgaag aattccagca aagtaaggca
     1021 gaaaaattaa acaatgccat taccgccttt caggaggctt taaaggtttt taacagtgaa
     1081 acttacccga tggattatgc catgacccag aataacctgg gcgcttccta tagtttgatg
     1141 gcagaggtcg atgataagga tcattgcctg gacaatgcga ccattgcttt ccaggaggct
     1201 ttaaagatct acaccccgga agcggatccc gagaataata aaaaggtcaa aaccaatctg
     1261 gatcggatca aacagcgttt tgggaaggcg taaaaataag gcggcggctg aacacttcag
     1321 ccaccgcctt tgattttcga cccgattttt actccccgtt ctaacgttcc cgagcgtgtt
     1381 tccccaagtt ctcgatattc atctgatccc ggatctcggc cagttcactc tcgaatttct
     1441 ttaattcctc aataatctca ttgaaccgtt tcgaaaaacg gggatcgttc ctgccgactt
     1501 cgtttaataa gaggcgggta tcctcaaatc ggttgattgc ttgaataatt ttagtttctt
     1561 ccagtacacc cattaagaac gctccttgtt agtgttttgg ctccgttccg tgacggtcct
     1621 tgctagataa aataaatacg attccctctt taacctcgct ctataagtat aatccaaatt
     1681 gaacccctta gccaagcttt ctttgaaaat taatatgaaa attttgcagc gcccctttta
     1741 gggaacacct ccttcgggag gcgggtactg tgcgccgatc acatcaaata gtcgcatctc
     1801 tcgccaaaag gtttcggtgg taccggttga tagagcccta tccgggctgc aaccgggaac
     1861 caagtctcgg ggtgataacc ggaggatgtt tggaaagaga aaggaaccgt aaacccgact
     1921 atttcttcct cgccggcatc ctgatccgat ggatcatcat caaaaaaacc acctactgcg
     1981 gcagggcgat tacgggtgtt taccatatgc ggatttggga tactattctt tccggccagg
     2041 cgggttaagg cgtgggaacc ggagagtatc atgtattcac tggcttgtat agagcgtttt
     2101 ttgagtgcca aaagacttaa gctgttaaac aacgattctt gatgttcttt gaaactgaca
     2161 acgggatccg ggttgattac agctacatct gtcggacgtt ctggttccaa ttcgaaagga
     2221 tttgtttctc cggaaaaaga ttggtccgtt tcaacggcca acacggttgc cgattccggc
     2281 cgttcacccc gggcgggatt aacggcccaa tcggtttgtt gtctcatacg atttatggca
     2341 ccctggtcgt ttcgctcccg tacctcccgg gaagaaattg aaccagcgtt cataagttcc
     2401 attgcccgag gctcagcccc ggttgttaca aaaaaactaa gctgattccg ctgccagagt
     2461 ggaacaggtt tcggacgatt gggccaagat ggtttcccaa ggatagcctc ggatatcacg
     2521 gcttggcccg acatgaagtt ttctgcggta aaacccgtca tttacactcc cctccgcggc
     2581 cggaacatcg caacattgat cgatctattt acttccgtaa tccattataa atgacccacc
     2641 ggggcttgtc aaaagaagga gtctcgacta aaaatcatag aaaacattgt actgccaaca
     2701 aagtaccctt ataacttggc gataagcttc ttcaactccg accccgtctc ctcatccgag
     2761 aggatcgcca gtgcagtgag gcagtttttt attaaagcct tatctatctt gggccccaac
     2821 gtcaaggaga aacgatgtac cagatcgagc ttgtcagcgt ccaaagaact ccctccctgg
     2881 tatgtatatc ttaaatcatc ttattcccca tgtcgggcgc cggaactagt agagcgtcac
     2941 gtaaataaag tgatttactt ttgcatacgc aaatagtcct gaatcacctt taagaatgtt
     3001 gcgccgtact tggtgagctt tgcctcgcca acccccttta cacccaacat catttcggca
     3061 gtggtgggtt tgacccgggc tagttcacta agagtactat cagaaaagat gatatacggc
     3121 gggaccattt cggatgcagc aatctcacgc cgtgtctgcc ggagtaactg gaataactgt
     3181 gcgctggcgc gcgaatcatc ggcggtcgct ttggttggtc tcttggggac cctccggaaa
     3241 accggctcct gatttttcaa tacggccaca gcctttggcg acaatttaac caccggaaac
     3301 tttcctgcgg ataaacgaag atacccatcg gccaccaata gattcagcaa atcccggatc
     3361 tcctcaagcg acagatcctg cataatgccg taagttgata gctgatccat tttagtggcg
     3421 cggatctttt gatttctggc acctttaagc accgctgcca ccatggcggt cccatagcgc
     3481 tcatgcatcc tcaagataca ggagaagatc ttttgggcgc ccgtggtgat gtcgatttgc
     3541 tcaccctcgt tacatacact acaattacca caggtttcgg gtgcgtgggg ttcgccgaaa
     3601 tattccagaa taaaccgccg caagcacttg gaggtatgag cgtaatccgc catgttctga
     3661 agtttccgat attcgttggc cttgcggacg ggagaataaa ccgactgttc gatgaggaat
     3721 ttctggacta cgacatcctg ggcgccaaa
//
LOCUS       DASVMB010000029         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_518779,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000029 DASVMB010000000
VERSION     DASVMB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518779"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF531_00925"
     CDS             complement(<1..246)
                     /locus_tag="VF531_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7712555.1"
                     /translation="MISELTVISGRDKSGNDENFTELTLEAGETISIVGPTGSGKTAL
                     ITDIELLAQGDTATKRRILIDQKVPDEAIRYNPALKPI"
     gene            549..1379
                     /locus_tag="VF531_00930"
     CDS             549..1379
                     /locus_tag="VF531_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX7712556.1"
                     /translation="MHRFSRSELLIGASGLTKLARSRVAVIGLGGVGSYTVEALARAG
                     VGSLLLVDYDEICLTNTNRQLHAIAGNYGRPKVEVMAERARNINPEIQVTALKEFLTP
                     DNIGSVLTPDCSYVVDAIDTVGAKVALITYCLTQKIPVISAMGTGNKFDPLAFKIEDI
                     SRTHTDPLAKAVRKSLREKGITHGLKVVFSTEKPIEPRSDVLTCHGGCICPHRDQQLW
                     NCTKRRSIPGSMPYLPPITGLILAGEVIREILAGEPVQTAENSAIPATQQFSETGMIS
                     "
     gene            1379..2542
                     /locus_tag="VF531_00935"
     CDS             1379..2542
                     /locus_tag="VF531_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7712557.1"
                     /translation="MEVYLDNSATTRPSPVVVEAVHRALTEVYANPSSLHHLGLEAEH
                     LVKAAREQVASAIVAAPEEIYFTSGGTEANNLAIFGAVSRVRHRGNVLVTTAIEHPSV
                     LNVFKELEREDYHVIHLRVDRFGLVDLEQLAGILASNPPIFISVMGVNNELGTIEPIA
                     AIGRMLAGMNPRPIFHVDAVQALGKIPLNVRQLKIDLLSMSSHKIHGLKGTGALFVKK
                     GTNLRPLLFGGGQESGLRSGTENVPGIVGMGVAAAQAASAFEINHQKMRARRDQLAQG
                     ILAGIPGVGINSPMDETGAPHILNVSFEGIRAEVLLHALEEDGIYVSTVSACSSRKKA
                     HSHVLQAAGLTSGQMEGAIRFSFSVDNSEDEIEYVIDRLSERVAELRRIMKGW"
     gene            2544..3725
                     /gene="thiI"
                     /locus_tag="VF531_00940"
     CDS             2544..3725
                     /gene="thiI"
                     /locus_tag="VF531_00940"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX7712558.1"
                     /translation="MRPLLIARYGEIALKGLNRSYFEDTLVKNIKRALYGLAFGQIYK
                     ADRRIYIDLAEDVDPEVIATRVSRVFGVVSVSLAERLELSFEVISAKALEMVNVELQG
                     RGASERPVSFKVECRRINKEFPLGSLDLMREVGAFILERQPNLRVDVHHPELTVYIEI
                     REQAYLYTRKIPGFGGMPYGTNGKALMLLSGGIDSPVAGWMMAKRGVELAAVHFHSYP
                     FTSERARDKVIELLKVLSGYCGRRINLHLVNLLDIQRAIREGCPEEYFTILSRRFMMY
                     LAEAIARNEECAALITGESIGQVASQTILGLQVTNSAVQLPVFRPLIAMDKVEITDYA
                     TRIGTYEISIQPYEDCCTVFLPKRPATKPRLDKVLKLEANLDREGLIATALQNAETRL
                     I"
     gene            complement(3949..4767)
                     /gene="larE"
                     /locus_tag="VF531_00945"
     CDS             complement(3949..4767)
                     /gene="larE"
                     /locus_tag="VF531_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX7712559.1"
                     /translation="MLSLDKLQALKELLSTFSGALIAYSGGVDSTFLAVIAHEVLGER
                     FLAVTAVSPTYPEHQLTEAEELAKRLGLPHKVIHSNEFSEPQYVANPPERCYYCKTAL
                     FQELKHLAAERGLEVVLDGANLDDLGDYRPGHRAAEELGVRSPLQEAGFTKQEIRDYS
                     KALALPTWDKPAYACLASRIPYGREITPEALARIDRGEQFLAGLGFTQLRLRDHYPVA
                     RIEVAPDEMGRAWEHRQAIAARLHEIGFPYVTIDLDGFRSGSMNETIGRKPSPG"
     gene            complement(4987..5664)
                     /locus_tag="VF531_00950"
     CDS             complement(4987..5664)
                     /locus_tag="VF531_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7712560.1"
                     /translation="MEDIDQRVRAIVGKELSCAAHNLDHVERVYRLCLVLAEGERDLD
                     LEILKLAALLHDIARVREDRDDSGQTDHAVLGAEMAGKILRDLDYPPERATRVAECIT
                     THRFRSGKAPQTKEAQILFDADKLDVIGAIGVARSFMLAGEYGEKLYSDEPLADYVAH
                     NLGGGKPLGRVKQVREHTSNLEYELKLKHIPERLYTSKARQIAADRVAFMDRFFERLK
                     LEITGQC"
BASE COUNT         1415 a         1639 c         1581 g         1475 t
ORIGIN      
        1 aatcggttta agcgcaggat tgtatcggat cgcttcgtcg ggtacctttt gatctatcaa
       61 aatccgccgt ttcgtggcgg tatcaccttg cgccaacagt tcaatatcgg tgatcagcgc
      121 ggttttaccg cttccggtcg ggccgacgat ggaaatggtc tccccggcct caagggttaa
      181 ttcggtgaag ttttcgtcgt ttcccgattt atcccggcct gaaatcaccg tcaattcgga
      241 aatcatacct atcccacctt tggtaagtca gatttatttt aggttttaga cttgccccta
      301 ctttcaatct tgtttaatgt atgctaaggt ccggtccccg gtcaccaaat gctctccgcc
      361 ccggtggaat atgccaaaat cccagaaggc ggccttgctt gacgccctcg aggcgatagt
      421 ggtattatta attgaacttt ttcggcaaaa acagatttgt tttatcatat ttcccttgaa
      481 tagcctggca ttgatcggtg aatattgaga ttcagtatgc ggaggtcttt agattgaacg
      541 aaggtaagat gcaccgcttc agccgttccg aattgttgat cggagcttcc ggcctgacca
      601 aactggcccg aagccgggtg gcagtgatcg gcctcggcgg ggtgggttcc tatactgtcg
      661 aagccttggc ccgcgcggga gtcggctcgt tacttttggt ggactatgat gagatctgtc
      721 tcaccaacac caaccgccag ttacacgcga tcgccggtaa ttatggccgc cccaaggttg
      781 aggtcatggc ggagcgcgcc cggaatatta atcccgagat ccaagtgact gccttaaaag
      841 aattccttac ccccgataat atcggctccg tattgacacc ggactgctcc tacgtggtag
      901 acgccattga caccgtaggc gccaaagtgg ccttgatcac ctattgcctc acgcaaaaaa
      961 ttccggttat ttcggcaatg ggaaccggaa ataaatttga cccgctggcc ttcaaaatcg
     1021 aggatatcag ccgcacccat acagatccgc tggccaaagc ggtgcgtaaa tccttacggg
     1081 aaaagggcat cacccatggc ttaaaagtgg tcttctccac cgagaaaccg attgaacccc
     1141 gttccgatgt attgacctgc catggtggct gtatctgccc tcaccgcgat caacaactct
     1201 ggaactgtac caagcgccgt tcgattccgg ggagtatgcc ctacctgccc cccattaccg
     1261 ggttgatcct ggccggcgag gtcatacgcg agatattagc cggggaaccg gttcaaacgg
     1321 cggaaaattc agctattccc gctacgcaac aattttctga aaccgggatg atctcctgat
     1381 ggaagtatat cttgataata gcgccaccac ccggcccagt ccggtggtgg ttgaagcagt
     1441 ccaccgggcc ttaaccgagg tttacgccaa cccgtcctcc ctccatcatc tggggcttga
     1501 agctgaacat ttggtcaaag ccgcccggga acaggtggct tctgccatcg tagctgcccc
     1561 ggaggagatt tacttcacct ccggcgggac cgaagccaat aatttggcga tcttcggcgc
     1621 ggtgagccgg gtacgccatc gcggcaacgt tcttgtgacc acagccatcg agcatccttc
     1681 ggtgctcaac gtgtttaagg agttggaacg ggaggattat cacgtaatcc acctgagagt
     1741 ggaccgtttt ggcctggtcg atctggaaca attagccgga atcctggcgt caaatccccc
     1801 catcttcatc agtgtgatgg gggtcaataa cgaactgggc accatcgagc ccatcgcagc
     1861 gatcggccgg atgctggccg ggatgaaccc ccggcccata ttccatgtag atgcagtcca
     1921 ggccttggga aaaatcccct tgaacgtccg gcagctaaag atcgacctgc tttccatgag
     1981 tagccataag attcacggcc ttaagggaac cggcgcacta ttcgtgaaga aagggaccaa
     2041 cctgcgcccg ctgctcttcg gcggcggtca ggaatccggt ctacgctccg gcacggagaa
     2101 cgtcccgggt atcgtcggta tgggtgtggc ggcagcccag gcggcttcgg catttgagat
     2161 aaaccaccag aaaatgcggg cgcgccggga tcaactggcc caaggaatat tggccggaat
     2221 tcccggggtg gggatcaata gtccaatgga cgaaactgga gcaccccata tcctcaacgt
     2281 ttcctttgag ggtatccgtg cggaagtgct cctccatgcc ttggaagaag acggcatcta
     2341 tgtatcgact gtttccgcct gcagctcgcg gaagaaagcc catagccatg tactgcaagc
     2401 cgccggtcta acctcagggc agatggaagg ggcaatacgg ttcagtttca gcgtggacaa
     2461 tagcgaagac gagatcgaat atgtaataga tcgcttatcg gaacgagtgg ctgagttgcg
     2521 ccggatcatg aaagggtggt aaggtgagac cgctattaat cgcgcgttat ggggagattg
     2581 ctctcaaagg attgaatcgt tcctactttg aggataccct ggtgaagaat attaaaaggg
     2641 ccctttacgg tttggctttt ggtcagattt ataaagcgga ccggcgaatc tatatcgatc
     2701 tggccgagga tgttgatccg gaagttatcg ccaccagagt cagtcgcgtt tttggagtag
     2761 tctcggttag tctggcggaa cgtttggagc tttccttcga ggtcatcagc gcaaaggctt
     2821 tggagatggt aaatgtcgag ttacagggaa gaggcgcgag cgagcgcccg gtctcgttca
     2881 aagtggaatg ccgccggatc aataaagaat tccctcttgg ctctttagac ctgatgaggg
     2941 aggtgggagc gtttatactg gaacgccagc ccaacctgcg ggtggatgtt caccatccgg
     3001 aactcactgt ttacatagag atccgggagc aagcctacct ctatacccgg aagattcccg
     3061 gatttggcgg catgccctat ggaaccaacg gcaaggcctt gatgctcctt tccggcggga
     3121 tcgatagccc ggtggccggg tggatgatgg ccaaacgcgg tgtggagttg gccgcggttc
     3181 atttccatag ttaccccttt accagcgaac gggcccggga caaggtgatt gaacttctta
     3241 aagtgttatc gggatattgt ggccgcagga tcaacctgca cctcgtcaac ctgctggata
     3301 ttcaacgcgc catccgggag gggtgtcccg aggagtactt caccattctc tcacggcgtt
     3361 tcatgatgta tctggcggag gccattgccc ggaacgaaga atgtgccgcg ttgatcaccg
     3421 gcgagagcat aggccaggtg gcgagccaga ccattctagg gctgcaagtc accaattccg
     3481 cggtgcaact cccggtgttc cggcccctca tcgccatgga caaggtggag atcacagact
     3541 acgccacccg gatcggaacc tacgagatct cgattcaacc atatgaggat tgctgcacgg
     3601 tatttctgcc caaacgtccg gcgaccaagc cgcgtctgga taaagtcctt aaactcgagg
     3661 ccaatctcga ccgcgaagga ctgattgcca cggcattaca aaacgccgaa accagattga
     3721 tttaactggt aaaggaacca cgaaacacac gaattttcac gaaaagatct cttgcaagac
     3781 cttatttttc gtgcctttag tgtgtttcgt ggttaaaaaa tccgttttat ccctaaattc
     3841 ccctgtccct cggctgaagt aactaataaa ccactaaaca catgaatttc acgaaaaggg
     3901 ttcttggagg atctgttctg gtctcctcta aggagaggta attcttagtc atcccgggga
     3961 tggcttccgg ccgatggtct cattcatgct cccggagcga aaaccatcaa gatctattgt
     4021 gacataagga aatccgatct cgtgcagtcg ggcggcaatg gcctgccggt gttcccaggc
     4081 gcgacccatt tcatccggag ccacctcgat ccgggctacc ggatagtgat cccgtagcct
     4141 gagttgggta aaacccaatc ccgccagaaa ttgttcaccg cgatcgatac gcgccaatgc
     4201 ctcgggagtg atctcccggc cgtaagggat tcgtgaagcc aggcaagcat aggctggttt
     4261 atcccaggtg ggaagggcaa gcgccttcga gtagtcccgg atctcctgct tggtaaatcc
     4321 cgcctcctgc aatgggctgc gcacccccag ttcctccgcg gcgcggtggc cggggcggta
     4381 atccccgaga tcgtccagat tggccccatc caatacgact tccagccccc gttccgctgc
     4441 aagatgcttc aattcctgaa acaacgccgt cttgcagtag taacaccgtt ccggagggtt
     4501 cgccacatat tggggctccg agaactcatt actgtggatg accttatggg gcaggcccaa
     4561 ccgtttggca agctcctcag cttcggtcag ttgatgctcg ggatacgtgg gcgatacggc
     4621 ggtaacagcc aggaatcgtt ctcccaaaac ctcatgggcg atgactgcca aaaaggtact
     4681 gtccactccg ccggagtatg caatcaaagc gccggagaag gtggaaagca gttctttcag
     4741 tgcttgcagt ttatccaagg atagcactcc tttgaaatct tcttaggcaa taggggccag
     4801 gcaataggca acagcaaacg attccgtatc caccttggag atttattttc tcccttttgc
     4861 ttgttgtctt ttatctcatg cccacgtctg ttgcctgttc cctgttgcct attgcctatt
     4921 ttacttattc ataccaaatc catgggaata atctatcata ccctgagggt ccggtcaagg
     4981 cccggtttag cattgacccg taatctccaa tttcaaccgt tcaaaaaaac ggtccatgaa
     5041 tgctacccga tccgcggcga tctgccgggc tttcgaggta tacagccgtt ccggaatgtg
     5101 tttcagtttc aactcatact ccaggttgga ggtgtgttcc cgaacctgct taacccggcc
     5161 caggggcttc cctccaccca aattatgagc cacgtaatcg gcaaggggct catcggaata
     5221 tagtttctct ccatactccc cggcgagcat gaaggagcgt gccactccaa tggctccaat
     5281 cacatccagt ttatcggcgt cgaagaggat ttgggcttcc ttcgtttgtg gcgcttttcc
     5341 acttcgaaaa cggtgagtgg tgatgcactc tgccacccgt gtcgcccgtt ccgggggata
     5401 atcaaggtcg cgcaaaatct tgcccgccat ctccgcaccc agtaccgcat ggtcggtctg
     5461 tcccgaatcg tcccgatcct cccgaaccct ggcgatatca tgcagcaaag cggctagttt
     5521 caggatctca aggtccaaat ctcgttcccc ctcggctaaa acaaggcaaa gtcgataaac
     5581 ccgttccacg tgatccaggt tatgcgcagc gcaggacagc tctttaccca cgatggcccg
     5641 aactcgctgg tcaatatctt ccatattcat cgccccaatg ttgattggat aactaccggt
     5701 tacggtagct taagtaataa cagtattcat cataatcttc ggaatcgatc ccgatatccc
     5761 tctcaaaatc ccggtgaaaa atttcccgac aatgattcct cggtgttact gactccgaaa
     5821 cccttttcat actccccgtt tatgccggaa cggcatacaa tgacaaaagc aatacaaatg
     5881 acccatatgt tatcattact gttaaatcaa taaaaaaggg ccagccatgg attgacaaac
     5941 cggacttgtt atggcattct aatttttata ggatttttta ctcattgaat agcttgtttt
     6001 ttcacggagg aatcatgaga agactttgga ccatggtgtt attcggtttg tttatttgtt
     6061 cgggcgtcct ggttgcggcg gtggataacg atattgcgaa ggctgatgaa
//
LOCUS       DASVMB010000030         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_150839,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000030 DASVMB010000000
VERSION     DASVMB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150839"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VF531_00955"
     CDS             <1..998
                     /locus_tag="VF531_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7712561.1"
                     /translation="KKGYHILLEKPISNNLRECVAIGEYAQKYPQVFSVCHVLRYTKF
                     FSTIKQLLDSGRIGKLVSIQHNENVAFWHQAHSFVRGNWRNSDESSPMILAKCCHDMD
                     ILLWLTGSSCRRISSFGSLAFFNPGNAPQGAPKRCLDGCPVINECAYAAPRIYLTENT
                     GWPTSVISNDTSREAREKALREGPYGRCVFYCDNNVVDHQVVNIEFENGVTAAFTMCA
                     FTKDCCRTIKLMGTQGEIRASDKSNEIAISDFKTGTTEVIDIERSEYGHGGGDYGIMH
                     DFIKLVAENDGKKGLTAIDFSVQSHLMAFAAEQSRIEGKIIDLNEFGNAMQLPKD"
     gene            1062..2042
                     /locus_tag="VF531_00960"
     CDS             1062..2042
                     /locus_tag="VF531_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7712562.1"
                     /translation="MFYLGVDGGGTKTVFLLMNEEGQIISHVTKGSSYYIQTQFAGFQ
                     RVLMEGISEVCHEADIEIDEINYSFLGIPAFGEAEQDSRRLGTIVQEIVRNGRFRCGN
                     DVEAGWAGSLACRPGINLVAGTGSIGFGKDPSGGTARAGGWGDFCGDEGSAYWLGTRL
                     LSLFGKEADGREAKTPLYDIVRSHFGINRDFDLISIVYDLLKLQRDEIAKLALLVFQA
                     AGQGDPKALALYHEAAYEHSLTVKSLLRQLHFDNGSDIPVSYSGGVFKAGEFILTPLK
                     KFLVDEPVRLIPPALLPVTGAALYAMKLDGQPLTEPLIDKLRKQEETIIR"
     gene            2039..>2918
                     /locus_tag="VF531_00965"
     CDS             2039..>2918
                     /locus_tag="VF531_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712563.1"
                     /translation="MKAYFASTIVTEKDSLPDAYLLEDQGFIAGIVREPPSGIPVEAY
                     KDCILAPGLIDVHTHGALGYETGFGSIEDLRRWSDFQLSHGVTGFLPTTASIPLERIR
                     QAADDVKYLAAQPLSNIWGLQMEGPFFAPTAKIGAQNPQYVRAEFGPEYKDFISEYRE
                     VIKYIAIDPVLKTAEAIVAFCAGLGIRIAAAHSMILYQDFMKCKRWGFSAVTHTFNGM
                     VGLDHRHPGLAYAACMDRDLYGEIICDGYHVNYSMIELFLQLKGYQRAILVTDSMGAA
                     GMPPGSYTLGDIHVNVS"
BASE COUNT          739 a          632 c          794 g          753 t
ORIGIN      
        1 taaagaaagg ttatcacata ttgcttgaaa agccgatctc caacaatctc cgggaatgtg
       61 ttgcaatcgg ggaatatgcc caaaaatatc cccaggtctt ctcggtgtgc catgtattac
      121 ggtataccaa atttttcagt accatcaaac aacttctgga cagcggcagg atagggaagc
      181 tggtttccat ccagcacaat gaaaatgtgg ccttctggca tcaggcccat agtttcgtcc
      241 ggggaaattg gagaaattcc gatgagtcca gtccgatgat cctggctaaa tgctgccatg
      301 atatggatat tttgctttgg ctcaccggtt cgtcctgccg tagaatttct tctttcggat
      361 cgttagcctt ctttaatccg ggcaatgctc cgcaaggcgc ccccaaacga tgccttgacg
      421 gatgtccggt tatcaatgaa tgcgcctatg cggcgccgcg gatatatctg acagagaata
      481 ccggctggcc gacttcggtg attagcaacg ataccagtcg ggaagcgagg gaaaaggctt
      541 tgcgggaagg accgtacggc cggtgtgtgt tttattgcga caataacgtc gtcgatcatc
      601 aggtagtcaa catcgagttt gaaaacgggg tcaccgcagc gtttaccatg tgcgctttta
      661 ccaaggattg ctgccggacc atcaaactga tggggaccca aggggaaatt cgggccagtg
      721 acaagtcgaa cgagatcgca atttccgatt tcaaaaccgg caccactgaa gtgatcgata
      781 ttgaaagatc ggagtacgga catggcggcg gcgactatgg cattatgcat gattttataa
      841 aattggtcgc tgagaatgac ggtaaaaagg gattaaccgc cattgatttc tcggtgcaaa
      901 gtcatctcat ggcttttgca gcggagcaat caagaatcga aggcaaaatc atcgatttga
      961 acgagtttgg aaatgccatg cagcttccaa aggattgaat tccgtatcaa ggaattgaaa
     1021 aacgcttgga gtagatcgtt attaaaaggc aggcgaggga tatattctat ctaggtgttg
     1081 acggaggcgg gaccaaaacc gtttttctcc tgatgaatga agaaggccaa atcatttctc
     1141 atgtcaccaa aggaagcagt tattatattc aaactcagtt tgccggtttt caaagagtat
     1201 tgatggaggg aatcagcgaa gtctgtcacg aagcggatat cgaaattgat gaaatcaatt
     1261 actccttttt gggaataccg gcattcggtg aggccgaaca agactctcgt cggctcggaa
     1321 caattgtgca agaaattgtc cggaatggcc ggttccgttg cgggaacgat gttgaggccg
     1381 gttgggcagg ttctctggcc tgccggcccg ggattaacct ggtagcgggc accgggtcca
     1441 ttggatttgg gaaagatccg tcgggtggca cggccagggc cggagggtgg ggcgattttt
     1501 gcggtgacga aggttccgca tactggctgg ggacacgatt actctcatta ttcggcaagg
     1561 aagcggatgg cagggaagcc aagactcctc tgtatgatat cgtccggagc catttcggga
     1621 tcaaccggga ttttgatctc atttctatcg tgtatgatct tttaaagctg caaagggacg
     1681 aaatcgctaa actagccctg ctggtatttc aagcggccgg gcagggcgac cccaaagcgc
     1741 ttgcgttata tcatgaagca gcttacgaac atagcttaac tgttaaatca ctattacgtc
     1801 agttacattt tgacaatgga tcggatatcc cggtttcata ctccggaggg gtgtttaaag
     1861 cgggtgaatt catcctaaca cctttgaaaa agtttttagt tgacgaaccg gtgcggttga
     1921 tacccccggc tttattaccg gtcaccggcg ccgcgcttta tgccatgaag ttggacgggc
     1981 aaccccttac cgaaccgcta attgacaaat tgagaaaaca ggaagagacg attatccgat
     2041 gaaggcctat tttgccagta caattgttac agagaaagat tctttaccgg atgcttatct
     2101 attggaggat cagggcttta ttgccgggat tgtcagggag ccgccgtcag gtatcccggt
     2161 tgaggcatat aaggactgta tcttggcgcc cggtttgatc gatgttcata cgcatggggc
     2221 attgggttat gaaacagggt ttggctccat tgaagattta cggagatggt cagatttcca
     2281 gttaagccat ggggttacgg gtttcctgcc gactacagcc tccattcccc tggaaagaat
     2341 caggcaggcg gcggatgatg ttaagtattt ggctgctcag cctctttcga acatttgggg
     2401 attgcagatg gaggggcctt tttttgcacc caccgccaag attggcgctc aaaatcctca
     2461 atatgtacgc gcagaatttg gcccggaata taaagatttt atctccgagt accgggaggt
     2521 aatcaagtat atcgcgatcg atccggtttt aaaaaccgcc gaggcaattg tcgctttctg
     2581 cgctggcctg ggaattcgca ttgcggcggc tcacagtatg attctgtatc aggattttat
     2641 gaaatgcaaa aggtgggggt tttccgcagt cacccataca ttcaacggga tggtggggct
     2701 ggatcaccgt cacccgggtt tggcgtacgc cgcttgtatg gaccgggatc tgtacggtga
     2761 aatcatctgt gacgggtatc atgtgaatta ttccatgatt gaattatttt tacaattaaa
     2821 gggctatcaa agagcgattt tggtaaccga ttcgatgggg gccgcgggaa tgccgccggg
     2881 atcatatacc ctgggggata tccatgtcaa tgtttcca
//
LOCUS       DASVMB010000031        24416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_150848,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000031 DASVMB010000000
VERSION     DASVMB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 24416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24416
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150848"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VF531_00970"
     CDS             <1..476
                     /locus_tag="VF531_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7712564.1"
                     /translation="GGFYNVIILNANYKAIPESLFESAKLDGANEFRIFSSIVMPLSK
                     PVIAALTVFTVVGIWNDYGTTLFFTQSQDLQTLQYFILKLIKSNAAVDQLTNIALSSN
                     PDVAALFTQAQGKGRVTAKTVELAAMVIASLPMIVMYPFAQKFFVKGVMIGSIKG"
     gene            527..1486
                     /locus_tag="VF531_00975"
     CDS             527..1486
                     /locus_tag="VF531_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7712565.1"
                     /translation="MEDAKKLRLPKTPLFRDPIYDGAADPTIIWNSEETSWWILYTNR
                     RANVPSFGVSWVHGTDIGVASSTDGGQNWLYRGTVSGLKFEPGRNTFWAPEVIRHEGI
                     YHMYVSYVKGVPSNWEWDREIIHYTSKDLWNWKFESKLKLSSDRVIDACVCKLPDGIW
                     RMWYKDEVHNSFTCAADSKDLYNWEVVGPVITGIPHEGPNVFYWKGKYWMIADFWRGL
                     MVQRSDDGVNWVRQRDILDKPGNRKDDNAYGQHPDILVLGDNAYIFYFTHPGACDQQN
                     IEEEMSYERKRTSLQVAKLELNGDELVCKRDEPFDFYLPEVNC"
     gene            1543..3912
                     /locus_tag="VF531_00980"
     CDS             1543..3912
                     /locus_tag="VF531_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7712566.1"
                     /translation="MNLQATLTVYTKEKGVALNDLYGVFFEDLNHAADGGLYAELVQN
                     RSFEFNPIDNPGYHALTAWEKIERGGGKAEIHVEESNPLNARNPHYLVIDVLEPGNGA
                     GIVNSGYNTGIPLKQGQNYKFSCYARRDFNWNTPLQIILESADGATYGEAAILVKASG
                     WVKYEATLTADTTDYNGRLAIITKGQGKLYLDMVSLFPEKTFKNRPNGMREDIARMIA
                     DMKPKFVRFPGGCLVHDGSLEPDARDSMYRWKNTLGDVAQRSARRNGWRYNQTFGLGY
                     YEYFLFCEDIGAKPLPVLPAGYDPHHRRIIPIEELQPWIDDALDLIEFANGDISTPWG
                     VRRAELGHPEPFGLEYLAIGNEEVGEPFFERYTHFHRAVKGKYPEIKIINTSGPFAAG
                     GEYERGWKSAQENKSDLVDEHYYQTPEWFLANYHRYDNFKADEPKVFLGEYASWGNTY
                     YNALVEAAYMTGLEKNVHAVGMACYAPLLCNADYVNWKPDMIWFNNHEVYGTANYYVQ
                     KLFMNHQGDHLLKVQANGFVPCKPRKGEPITGKISVAGDKTAARFYEIKLQNNDTGEM
                     KLYNDVLTDTESGLVELGETDWENYSLSLKARKGGGIKGFIISYGEKEPQNRLFWEIG
                     GWQNQDSLVSARVNGRNSCLTQSLFTVEANREYRLDLEVAGRHIRTYIDGELINDTED
                     KLLEIEQLYYSASAESSTGDVIIKVVNVQEQPVAAQVVLDGMKKKSIMGKIYEMSGYE
                     LNNENSFEYPQLVVVKQKKFSLDSHIFDYEFPKHSITVFRFTKIQKSEN"
     gene            3916..4209
                     /locus_tag="VF531_00985"
     CDS             3916..4209
                     /locus_tag="VF531_00985"
                     /inference="COORDINATES: protein motif:HMM:NF037003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712567.1"
                     /translation="MGKNEIAQTPYGAKQIIRTEDTVIWVSNDEDGSKYVAFFNTAFT
                     PTQPEISFMNLGIRGTYKARDLWTHEDVGEYTERISAKVNIHGAMIYKLTKIT"
     gene            4350..5801
                     /locus_tag="VF531_00990"
     CDS             4350..5801
                     /locus_tag="VF531_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX7712568.1"
                     /translation="MLNKANELSVYPVPEKVAVNSDFSVRVRCGGGEWEKLSAYNVKV
                     DMHDVRNASMVYFDFSGTVEVEVVKNTGTVESVMIRPLSFGVRHRQEGNKITFTLNKP
                     GKLSLEVNGDRFHNLHIFAGSPVKNTPDPEASNVLYLKPGIHRPQEILKAISSKNLIT
                     GREPEIIYFAPGMHYIEELILSIPSGKTVYIPGGAAVVGSIVCDHVQDVTIRGRGVLY
                     LINVERSSYFRGFRIIFSRNIRIEGIVVIDPPHYSIFIGQSEEVTIKDFKAFSCYGWS
                     DGIDCMSSSKIDIDDVFMRNSDDCIAIYGHRWEFNGDSRNITVKNSVLWADVAHPTLI
                     GTHGDHENNGNVLEDITFENIDILEHHEPQPNYLGCMAINAGDKNTVRHVRYENIRVE
                     QFENGRLFDIRVFKNPDYNPAPGTRIENIHFKDIFYNGIGEHPSRIMGYDDDRVVQDV
                     TVENLRINGKIILDEKSGNIDIGESVYGVVFKR"
     gene            5837..7705
                     /locus_tag="VF531_00995"
     CDS             5837..7705
                     /locus_tag="VF531_00995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7712569.1"
                     /translation="MARKPLFLRSIFLKFSISFIIIGLIPLLFFSFIAIDTFSKNIEE
                     VTIHSQEQLLGYAGENIGKLIAGYNDLTKLVYTYNSDSYGEPLNNLLKRAAETHSKSI
                     SAIQSTLAINDFVRSLLYTDAYIQNVIFIDERMRAYDASQMSKVFNTDYDFYNKGRLK
                     YIIQNRNQLTILPSHKAEYYKGSSELVISFARNFYDLSQIESHKILGTIQIDVDVNTF
                     DTVFNHLTTQSEDEIYLVDNDGYCIYSNLREKIAKNMGWFQQKKTKFTNKNFTGYFLD
                     EQVYYIYSKVPTQNWVIIRKIYQKDILNKVQGIRNFLKIIITVCSMGLILVAIVFSRR
                     FSIPVQRMLRQMKKVERGDLNSRVTINSQDEMGQLAEGFNNMVCELQRYIAKSYLAQN
                     KQKEAELNALKTQIQPHFLYNTLEVIRMSAVDHGDEQVAEMVHSLAAQLRYLIGCNDD
                     LVTLGQELEMVENYFRLIRIRYEDKIDLEIRIPEQIRHYHILKLTLQPVVENAVVHGL
                     KPKTSHGKVLITGEIAGGHLVLNVLDDGIGMTEATLAGINDLLVGEKIGEPTAGGWKN
                     VGLKNVHDRLRLRFGEPYGIDLKSQALIGTVVRIITPIISDINNSGGVNHAQDGAG"
     gene            7683..9293
                     /locus_tag="VF531_01000"
     CDS             7683..9293
                     /locus_tag="VF531_01000"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7712570.1"
                     /translation="MLKMVLADDEPLILKGLKKIINWSEYGIEITGEAANGPQAEQLL
                     LSEKPDIFLSDICMPGKNGIELLQMLHDRNINTKVILLSGYQEFSYAKEAIAFGALEY
                     ILKPIDKEQLAGAIRKASSLIRKEREETGLREKLARYEMERTDTILENAWEHLIAGTI
                     SPPEIKVLAPDIGDGFYTVVNIIIDELFDNPLAFAAGEIQLIQYSVCTLIQKMLEEKK
                     AGHALMKGQQITLVLCYPEKRPAPTEAQDFAGEVGRMVKNQMMKTVTIGIGEMVSGLE
                     LVPRSYQSALRAFEDRFFAGNDRIIMATQEHIPKYQVEDLYLEQKKLCNGLLTNEREL
                     FDESLANIKYIVSDIAWGDKETAIGYYLTTLANLSRELSGYGIDLESLADKTGRISKE
                     LKGLATLNELHSWLTKYLDEITRRIGFQIKSSEPQVIKKVKKYIEDDYNHDITLEAVA
                     GIACMNPNYFSVFFKNHTGENFRDFLLRIRMEKALGILLSTECKTYEIADQVGFNDPK
                     HFSEMFKKYYGKNPMEYKKDFLKKPTLS"
     gene            9373..10692
                     /locus_tag="VF531_01005"
     CDS             9373..10692
                     /locus_tag="VF531_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7712571.1"
                     /translation="MARRGWMGFVLIGLILSLFSFNLTMAAPKETKLTCIYATGDPLT
                     KQLVHETIVKFMQRNPGIKISEDLSINTGAYADALKIKDSVGEFPDFVEMRDAPMFVR
                     AEKLAPLPKELMGLYETTIPIYGTVYTAPFMAAAPNGVYYNKTFFKKNGLKEPKTYAE
                     FLQLCEKIKAKGVSPLVAGIKDIWHMGFLFGQFWVTDIASKNPNWIADRYAGKVHFTD
                     ADFADAMKQLVTLFTKGYVEPGYMSTTEAQCPSILVSGKVAMYYCGPHVIKQITDADP
                     SFDLGWFPIPDAKGKVYLLGGATKDGWAISAQAAKDPAKVQAFVKFVKFFMAKPQYSK
                     FVASGNYFPTSKQAITYKATPAMQSVLKAYAKAGKSLNWNQGVGANEIPPAFRNWTYK
                     KVQEMLIGTVSVEDGLKQMDEEFDKEARDFNPTKFVPEKLSVPTATK"
     gene            10770..11657
                     /locus_tag="VF531_01010"
     CDS             10770..11657
                     /locus_tag="VF531_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712572.1"
                     /translation="MAKKRRLISQVSYGMLLPAFLLYTGFVIVPALSSFYYSLTSWDG
                     LSNMMPFIGLRNYMDMFHDIRFFTAVKNTMFIAIAATILENAVALGLALLVDRIKIFK
                     SFFRSVFYIPVLLSGIITGFIWSVMMNYSFGVINQFFAMIHLDILRLNWLGDPKIALI
                     SILLTMIWKGAGYYMIIYLAGLQGIPQEILEASRIDGASGLQQFRFITFPMLAGAVTI
                     NMTLSLIGGLKVFDQVAVMTDGGPGFATETIAYQIFKVAFADSKQGYGTAVAVMLFAI
                     TLVFSAIQLALLRKREVEL"
     gene            11658..12524
                     /locus_tag="VF531_01015"
     CDS             11658..12524
                     /locus_tag="VF531_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712573.1"
                     /translation="MGTTIDLKQPFDEKRIGKTHDILLVILVAFLAALFFYPVYFSII
                     SAFKTQGEIMRDAMAFPSALRLDNFIYLWNETRFPQAVVMSAFLTVTSTCLMILVIPM
                     AAYVIERRNSKLTGLIYIFFISGMMIPFQAYMIPLFRQLKWLHLFGTVWGPIVVYISG
                     ATAFGTLLFTSFIKGIPREIEESAAIDGCSSFNTFWQIVFPLLKPCTASMVILQGLGI
                     WNDFLMPLLVLPSDQAKTINVEIYSFVSELATRWDVLFAGSFCSMIPIILIFGFLQKY
                     FVKGITAGTVKG"
     gene            12551..14131
                     /locus_tag="VF531_01020"
     CDS             12551..14131
                     /locus_tag="VF531_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX7712574.1"
                     /translation="MGQIINPILRGFNPDPSIIRVGDDYYIATSTFEWFPGVQIHYSK
                     DLIHWKPVAHPLNRLSQLNMAGIDSSCGVWAPNLSYDRGTFYLIYTIVRSTGGIFKDT
                     LNFLVTAADIQGEWSEPIFLNASGFDPSLFHDDDGRKWLVNMDWNHGQGKKRFGGILL
                     QEYAPVQKRLVGPVQNIFQHSGLAEGPNLYKRDDYYYLMLAEGGTGLNHSVWMARSKS
                     IAGPYETDLKNPILTAREDPRIPLQKAGHASLVHTPSGEWYMAHLCGRPIPASGRCTL
                     GRETCIQKVIWSDDGWLRLAQGGNKPLMTVPGPDLPEYPFPQESVKDDFDSEKLGIHF
                     QTLRVPLGEEYLSLIERPGYLRLKGGESLNSRYFQSLIARRQQAFCYTATACMEFEPE
                     SYKQMAGLICIYDIHNYFYLRVSHDGTGKCLAIINCDNGTYNYPLEKDIYIGGDSRHY
                     LQARVDYDRLRFYYSSDGEQWLQVGGNFDYSRISDEGINKLAFTGAMIGICCQDLSGM
                     RKHADFDYFEYIERDFAG"
     gene            14307..15410
                     /locus_tag="VF531_01025"
     CDS             14307..15410
                     /locus_tag="VF531_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7712575.1"
                     /translation="MFNQKEALERVLRKLERTSSRIQDEIPYRTSGGKYDDYSAKIDW
                     WTNGFWPGILWLAYRQTQKREYADWALRVEEKMDAAFREFYHIHHDAGFMWHISAVAD
                     YKLTGNPASAKRGYLAASVLASRFNPAGSFIRAWNDDCTGWAIIDCMMNLAILYWASG
                     YIHDPRFSNIALAHAATAQKYFIREDGSARHICVFDPVSGEYLENLGGQGYGVDSAWS
                     RGAAWALYGFAMSAKYSGRPDFLNTAKRVANFFVAHLPEDSVPFADFKAPADINIHKD
                     SSAAAIAAGGLLLLSRLVPEDEKPFYRDSAVKIVSSLYDNYTDWDGDEALIQKGCTAF
                     HSKPEEGLSTSLIYGDYFFLEALMQLNGFEGLF"
     gene            complement(15477..17111)
                     /locus_tag="VF531_01030"
     CDS             complement(15477..17111)
                     /locus_tag="VF531_01030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7712576.1"
                     /translation="MTKQPIFSYTKSTFYKIWLAICALIIVATVASSFLVYLQVNNQL
                     KTNMEQRLLWEANFYRQQLDEVFLQAALKLDNLVRSAVATTADRTLLQNEMDTIRTQT
                     PSVIRSWVAYPGGILVPSPNTRPDYVKNLPWWHEYLSGKTPQNFTGFQIGRGRSLVGK
                     PFIDQSNFTALVPLLSFDLHEIQIIRAAGAELDLNIALTENTGVDIDWSTIPVSIYTT
                     DGILVACPYRYYSGNLNLLNKISDHPLIRQMLSYPNEVHGFGTYKKNDRKMAGVYLKD
                     PLLGLVLTVEYPAAEVVDPVRRIASGPLIITALLLLIATILIATIYANTKRLRQVEQL
                     ARSAELRALQAHINPHFLFNTLDCLVGLAVSTGNTSLVNMIRSLIHVFRYTTRKMAEL
                     VTLQEELDYLKEYISLQQIRYGSRFSFELSVPEELLKTKIFKFCIQPLVENCFIHGVE
                     KSLDPVLIRVQANKSENDLEISVSDNGPGMTMERIREINKSLELETYESGSQGNGVGI
                     SNIHHRLKYAYGNAYGITLIPLEPGLSVYLRIPINQ"
     gene            complement(17148..17957)
                     /locus_tag="VF531_01035"
     CDS             complement(17148..17957)
                     /locus_tag="VF531_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7712577.1"
                     /translation="MNALKGGVGLFQVMVVDDESSALEKLTNYIHQADLDFHVIAKAL
                     GAEDALYFLGMTKPDLVITDIKMPVTDGLSLLRQMRQVGWDGYAAIITGYDDFAYAQQ
                     AIRLNVFEYMLKPVFPEDVAALLKRTKELLERDQTKKAQMRSKIQAELQGESLTIAEK
                     GCIPAYIEQAKEYIKDHYAEPLTLIQVAKFVSVNPVYLSSRFAKYCGQNFLEYLTYYR
                     INKAEELLTHSILQIQEVATKVGYTDIAYFSRVFRRETGFTPSNYRSNFKK"
     gene            18129..19409
                     /locus_tag="VF531_01040"
     CDS             18129..19409
                     /locus_tag="VF531_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7712578.1"
                     /translation="MKKWFRLTGCLVLLIAFFSTLASAENTVTIQYAFWGNPTAIGVE
                     KDIIDEFEKTHPNIKVTPVVAAYNDYHTKLLTMYAGGQAPDVMRIDSYYMADFMKNKA
                     LKDITGLIKQDKLNMNSYYPAGLLDCMQGNRYYGLPWGTAPVFMFINVKMFKDTGIPV
                     PSVDWKYEDFLKIARQLSKGEGMDRQYGFAVGTTDLTNLFPFIWAGGGDLFDKNRRKF
                     TLNKPQAVQKIQEIANMIKEGIIPDPAQFTSGDVFNRWSANYKLAMRMGTAAEILSMQ
                     SIAGYEFEVLPFPGTVKYPRTTISKSNVVGISSTTKQAKAAWEFLKFLRGPGQPGEVL
                     YMKAKRVPPSFDDPELWKLYADPAKSPKMVVESSQAIAVKYGHVLPLRTGWIEIQSLL
                     LPELQRVYAGQITAEKAMNEIAPKIQEVMDRTGK"
     gene            19545..20426
                     /locus_tag="VF531_01045"
     CDS             19545..20426
                     /locus_tag="VF531_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712579.1"
                     /translation="MKRQSTRFREAVVGYLFLTPNFLGFVIFTIFPVIGSLLISFSDW
                     NLITSPAAVGLTNYANLFHDQVFMQSLTNTVIYSFVSVMLNIILALFLAILLNKKIHG
                     LSFFRAAYFLPVVYSSVAVALIWQWLFDYQMGLLNHFLSWFQLGPFPWINSPQWALPS
                     VILVSVWKGMGYNMVIFLAALQGVPGELYEVAKIDGANAWKTFWNVTWPMISPSTFFV
                     TVTSIINSFQVFDLTTVLTNGGPANATNTLVMLVFQHAFRFFRMGYASAIAYTLFGIV
                     FIFTILQTVVAKRWVHY"
     gene            20463..21326
                     /locus_tag="VF531_01050"
     CDS             20463..21326
                     /locus_tag="VF531_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712580.1"
                     /translation="MKTSGLMIEKPHNYFDTKFLWQLTVYLVLVIGALMVLFPLVWAL
                     IASVKSYTEIFNNPLGLPREIVWQNFIQVFEAVPFHLYFLNTLKITAACTLGTVFTSA
                     IAAYAFARLRFPGRDFLFMGYLATLMIPRQITLVPTFIIMRWLGLLDNHLSLILPGLF
                     SAYGTFLLRQFFLTIPVELEEAAIIDGCGYLRRFARIIMPLSKPALATLGIFTLMGTW
                     NDYLYPMVFLNSENNRTLVMGLAIFRGDVDVQWNLLMAAVVMTSAPIVIAFILAQRFF
                     VEGIATTGLKG"
     gene            21422..23515
                     /locus_tag="VF531_01055"
     CDS             21422..23515
                     /locus_tag="VF531_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEX7712581.1"
                     /translation="MVKKIAFVMDKDTLFYLPEVLTPPVGHAVRILFRDFEKVLGSAP
                     KQCDDIHSASVIIGYASEDDPIHNRPEAFAIRFENMDSEGSPVMHICGSDDLGIIYGL
                     LYISREYLGVEPFWFWADKEPKKLERIQIPIREYISPEPRVRYRGWFVNDEVCLIGWT
                     DSYPPPQEVWLPVFEALLRCGGNMVIPGTDLPRNGIHWELASEMGLYITQHHAEPLGA
                     EMFFRAYPHEEPSYDKNRVLFEGLWEDAVRKNRDKKVIWALGFRGQGDCPFWEQDPTY
                     STPERRGELISKAIRRQYELVSQYVENPACATYLYGEITELYRAGLLDLPDGIIKIWA
                     DNGYGKMVSRRQGNHNPRVPSLPAPTETGPHGLYYHITFHDLQASNHLTMFPSSPGLV
                     KDELLKAFRAGADRYLLLNSGNIKPHLYLLEIVSRLWNEGTIDIDEHLDDFCGRFFST
                     ARGKARECYQDYFHQTIQYGIYTDDKAGEEFYHHPPRSIIGHWIRNEITETACDLQWA
                     TGEVTFDEQVRCFFEKLIAAREGWRKLQEKARTTAAMLRKEEAVFFRDHLAFQVELHS
                     SGCKGFIALCQSYFAFREGDFPRAFVYASQSLWNYNDSIKAMAEAEHDRWRDFYRADW
                     LTNVKSTIYSLETLRRYLRMHGDSPEFFLWNKAYLMPESEKKIYLENTHRKPLSDDDL
                     ARKLKDKFGIPDEPQ"
     gene            23565..24203
                     /locus_tag="VF531_01060"
     CDS             23565..24203
                     /locus_tag="VF531_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEX7712582.1"
                     /translation="MNRFIKWRCQCIAKEMVEILKQKAYDAHYAENLEEARNIVLEMI
                     PHGSSVAVGGSETLAAMGLVDVFRNGDYKFFDRYQNLPFPEIVEIYRQSMLADFLVTG
                     TNAITRQGQLVNVDSSGNRVAGIIFGPRRVIVVAGANKVVADLDAAFKRLQEIAPMNA
                     LRNGHQTPCVKTGRCEDCQIPARMCNSIGIVNHGMKFEGRISVIMVAEEVGF"
BASE COUNT         6623 a         5187 c         6076 g         6530 t
ORIGIN      
        1 ttgggggttt ttataatgta attatattaa acgcgaatta taaagcgatt ccggaatcgc
       61 tttttgaatc ggccaaactg gacggagcca atgagttcag aattttttca tcgattgtta
      121 tgcctttatc aaagcctgtt attgcagctt tgactgtttt tacggtagta ggcatctgga
      181 acgattatgg aacaacctta ttctttaccc agtcccagga tttgcagacc ctgcaatatt
      241 ttattctaaa gttaataaaa tccaatgcgg cggtggatca attaaccaat attgctcttt
      301 cttcgaatcc ggatgtggcc gcacttttta cacaggccca gggaaaaggg agagtgacgg
      361 ccaaaaccgt tgaactggct gcgatggtca ttgcctctct gccgatgatt gtcatgtatc
      421 catttgcgca gaaatttttt gttaaaggtg taatgattgg ttccataaag ggataatttt
      481 cgattttatt cgtgaagcaa ttgaaatcga aaaggagatt tagcgaatgg aagacgcaaa
      541 aaagttaaga ttgcccaaaa cgcctttatt tcgggatccg atttacgatg gcgcggctga
      601 tccgacaatc atctggaata gtgaggagac aagctggtgg attttgtata ccaatcgaag
      661 ggcgaatgtt ccaagtttcg gagtaagttg ggtgcatgga acggatatcg gcgtggcttc
      721 atctaccgat ggaggccaaa actggcttta tcgggggacg gtatcgggac tcaagttcga
      781 acccggtcgg aacacctttt gggcaccgga ggtcatccgg catgagggta tctatcatat
      841 gtatgtcagt tatgttaaag gtgtaccgtc caattgggaa tgggacaggg agatcatcca
      901 ttataccagc aaagatttgt ggaattggaa gtttgaatcc aaattaaagc tttcttcgga
      961 ccgagttatt gatgcctgtg tctgcaagct ccccgacgga atctggcgaa tgtggtataa
     1021 ggatgaagtg cataattcat ttacatgcgc tgcggatagc aaggatttat acaattggga
     1081 ggttgttggt cctgtaataa ccggcatacc gcacgaaggc cccaatgttt tttactggaa
     1141 agggaagtac tggatgattg ctgatttttg gcgcggactt atggttcagc gatcggatga
     1201 cggagttaac tgggttagac aaagggatat actcgataag ccgggaaaca gaaaagacga
     1261 caatgcttat ggccagcatc ccgatatatt ggttctgggt gataatgcct acatatttta
     1321 ttttacccat ccaggcgcgt gcgatcagca aaacatcgaa gaggaaatga gctatgagcg
     1381 taaaaggaca tcgctgcaag tagcaaaatt ggaactgaat ggcgatgaat tggtatgtaa
     1441 gagagatgaa ccttttgatt tttatttacc ggaagtgaat tgttagccgg catgctatga
     1501 tcaagccgat cttccgatca aagaaagagg ctgagagaag caatgaattt acaggcaaca
     1561 ttaactgtat atacaaaaga aaaaggcgta gcattaaacg atttgtacgg tgtctttttt
     1621 gaagatttaa atcatgcggc cgacggcggg ctttatgcag aacttgtgca aaaccgttcc
     1681 tttgaattta accctattga caatccgggt tatcatgcgc tgactgcctg ggaaaaaatt
     1741 gagcgcggcg gcggaaaagc tgaaatccat gtggaggaga gtaatccgct aaatgcgcga
     1801 aatccgcatt atcttgtcat tgatgtttta gaaccgggca acggtgccgg tattgtgaac
     1861 tcagggtaca ataccggcat cccgcttaag cagggacaaa attataaatt ttcctgttac
     1921 gcaaggcggg attttaactg gaatacaccg cttcaaataa tcctggaaag cgcagacggt
     1981 gcgacatacg gcgaagcggc aattttagtc aaagcttccg gatgggtaaa atatgaggcg
     2041 actctcacag ccgatactac cgactacaac ggtcgtctgg caatcatcac aaaaggccaa
     2101 ggaaaactat atctcgatat ggtatccctg tttccggaaa aaacctttaa aaatcgcccg
     2161 aacggtatgc gtgaggatat tgcccggatg attgccgata tgaaaccgaa atttgtgcga
     2221 tttccaggtg gatgcctggt acatgacggc tctcttgagc cggatgccag agactccatg
     2281 taccgctgga agaatacgct cggcgatgta gcccaaaggt cggcgaggcg gaatggctgg
     2341 agatacaatc aaactttcgg gttaggctat tacgaatact tcctgttctg tgaagatatc
     2401 ggggcaaagc ctcttccggt gcttccggcc ggatatgacc cgcatcatcg gcgtattatt
     2461 cctattgagg aattacagcc ctggatagac gatgcgcttg atttaatcga atttgccaac
     2521 ggtgatattt ccaccccctg gggcgtcaga agagctgagt tgggtcatcc ggagccgttt
     2581 ggtctggaat atcttgcgat tgggaacgaa gaagtaggag aaccgttctt tgaacgctat
     2641 acccattttc atagagccgt taagggaaaa tatccggaga ttaaaatcat caacaccagc
     2701 ggtccttttg cagcgggcgg tgaatatgaa cgcggctgga aatccgccca agagaacaag
     2761 tccgatttgg tagacgagca ttattatcag acaccggaat ggttcttggc aaattatcac
     2821 cgatatgaca attttaaagc ggatgaacct aaagtatttt taggcgaata tgcttcatgg
     2881 ggaaatacct actataatgc ccttgtagaa gctgcttata tgaccggttt ggaaaagaat
     2941 gttcatgcgg tagggatggc gtgttatgca ccgctgctct gtaatgccga ctatgtgaac
     3001 tggaagccgg atatgatttg gtttaacaat catgaggttt acggtacggc aaattactac
     3061 gtgcaaaagc ttttcatgaa tcatcagggg gatcatttgt tgaaagtcca agcgaacggt
     3121 ttcgttccat gcaaaccacg gaaaggtgag cctattaccg gcaaaatatc cgttgcgggc
     3181 gacaagacgg ctgcccggtt ttatgagatc aaactacaga ataacgatac cggtgaaatg
     3241 aaactgtata acgatgtttt gaccgacacg gagtccggtt tggttgagct gggtgaaacg
     3301 gactgggaga attattcgtt gagtttgaag gcaaggaaag gcggtggaat aaaaggattt
     3361 atcatcagtt atggcgaaaa agaacctcaa aaccgccttt tttgggagat tggcgggtgg
     3421 cagaatcaag actctctggt atctgcacgt gtaaacggaa ggaattcctg cttaacacag
     3481 agcttgttta ctgtcgaggc gaacagggaa tataggctgg atttagaagt tgcaggaagg
     3541 catattcgca cttatattga cggggaatta attaacgata ccgaagacaa actgcttgaa
     3601 atagaacagc tttattactc cgccagcgct gaaagttcga ccggtgatgt tatcatcaag
     3661 gtggtaaacg tgcaggaaca accggttgca gcacaagtgg ttttggatgg catgaaaaag
     3721 aaatcgataa tggggaaaat atatgaaatg tcaggctatg agcttaataa tgaaaacagc
     3781 tttgaatatc cgcagcttgt agtggtaaaa caaaaaaaat ttagtttaga cagtcatatt
     3841 tttgattatg aatttccgaa gcattcgatt actgtttttc gatttacaaa gattcagaaa
     3901 agtgagaact aaggcatggg taaaaatgaa attgcacaaa caccatatgg cgctaaacaa
     3961 attatccgta cggaagatac agtaatatgg gttagcaacg atgaagacgg gagtaaatat
     4021 gtggcttttt ttaatacggc atttactcca acacaacctg aaataagctt tatgaatctt
     4081 ggaatcagag gcacatataa agctagagat ctctggacgc atgaagatgt tggagaatat
     4141 acagaaagaa tcagtgcaaa agtaaacatt catggagcaa tgatttacaa actgacgaaa
     4201 attacgtaaa gcaaggtaag caaatgaaat aattaaaaca ttctgataat gcggctttag
     4261 aaagggtact tacagatttt aaattatcac aggttaaagt taaacttgag gtattcagcg
     4321 aattcatatg aaattccggg agaattgtaa tgttaaacaa ggcgaatgaa ctaagtgtgt
     4381 accctgtacc tgaaaaggtt gcagtgaaca gcgacttttc ggtcagggta aggtgcggcg
     4441 gaggggaatg ggaaaaatta tccgcctata atgtaaaggt tgatatgcat gatgtccgga
     4501 atgcgtccat ggtatatttc gacttttccg gaacggtcga ggtggaggtc gtaaaaaata
     4561 ccggaacggt ggagagtgtc atgattcgcc ctctttcttt cggtgttcgc catcgacagg
     4621 aggggaataa gattacgttt actctgaata agccgggaaa gctttcgctg gaagttaatg
     4681 gcgatcgttt tcataatctg catatttttg cgggtagtcc ggtgaaaaac acaccggatc
     4741 cggaagcatc aaatgtacta tacttgaaac ccggtatcca ccggccgcag gagattttaa
     4801 aagccatctc ttcaaaaaac ctcatcaccg gcagggaacc cgagatcatt tattttgcac
     4861 ccggaatgca ctatatcgag gagcttatat tgagtatccc ttcgggaaaa acagtatata
     4921 ttccaggagg agctgcggtc gtaggttcta ttgtgtgtga ccatgttcag gatgtcacca
     4981 tacgggggag aggtgtgctc tatctgatca atgttgagcg cagctcatat ttccggggct
     5041 ttcgaatcat tttctctaga aatattcgca ttgaaggaat tgtagtgatt gacccgcccc
     5101 attacagcat ttttatcgga cagtcggagg aggttacgat taaggacttc aaagccttct
     5161 cctgctacgg gtggtctgac ggaatcgact gtatgtccag ctcgaaaatt gatattgatg
     5221 atgtctttat gcggaattcc gacgactgta tagccatcta cggccacagg tgggaattca
     5281 acggcgacag caggaatata actgtaaaga attccgtctt gtgggcggat gtggcccacc
     5341 cgacacttat aggcactcac ggggatcacg aaaataacgg gaatgtgttg gaagatatta
     5401 cttttgaaaa tatcgatata cttgagcatc atgagcctca acccaactat ctgggctgta
     5461 tggcgataaa tgccggtgat aaaaatacgg tgaggcatgt caggtatgag aatataaggg
     5521 tggaacagtt tgaaaacggc aggttgtttg atatccgtgt gttcaagaac cccgactata
     5581 atccggcacc ggggacgaga attgaaaata ttcattttaa agatattttc tataatggaa
     5641 tcggtgagca tccctcacga attatgggat atgacgatga ccgggtggtg caggacgtta
     5701 ctgttgaaaa cctgcgaatc aacggaaaga taattctgga tgaaaaatcc gggaatattg
     5761 atatcggcga atctgtttac ggggttgtgt ttaaaagata ggcgatgaag cgataacttt
     5821 tggatgaggg tatatcatgg ccaggaaacc gttgtttttg cgaagcatct tcttgaagtt
     5881 tagcatttcc tttatcataa tcggtttgat tccgctgctt ttttttagtt tcatcgccat
     5941 cgataccttc tccaaaaata tcgaggaagt tactattcac agtcaggagc agctgcttgg
     6001 ctatgccgga gaaaacattg ggaaactaat tgccggttac aacgatctaa ccaagctggt
     6061 ttatacatac aattccgata gttacgggga gccgttgaat aatcttttga aacgggccgc
     6121 agagactcat tcaaaaagta tctccgcaat ccaatctacg ctggcgatta atgactttgt
     6181 ccgctcttta ttgtataccg atgcctatat ccaaaatgtc atctttatcg atgagcggat
     6241 gcgagcttat gatgccagcc aaatgtcaaa ggttttcaat accgactacg atttttataa
     6301 caaaggccgg ttaaaatata ttattcaaaa ccgaaaccaa cttactattc ttccctctca
     6361 taaagcggag tactataaag ggtcttccga actcgtcatc tcttttgcca gaaactttta
     6421 tgatctttcc cagattgaaa gtcacaagat acttgggaca atccagatcg acgtggatgt
     6481 caataccttt gatacggtct ttaatcattt gacaactcaa agcgaagatg aaatctactt
     6541 agtcgataat gacggttatt gcatctattc gaatctcagg gaaaagatcg ccaaaaatat
     6601 gggttggttc caacaaaaga agaccaaatt cacgaataaa aattttaccg gatatttcct
     6661 tgatgagcaa gtttattata tttatagcaa agtaccgacc caaaattggg tcattatccg
     6721 gaagatctat caaaaagata ttctaaataa ggttcagggc atcagaaatt tcttaaaaat
     6781 catcattact gtttgcagta tgggtctaat attggtggcg atcgtttttt ccagaaggtt
     6841 ttcgattccg gtccaaagga tgctccggca gatgaaaaag gtggagcggg gtgatctcaa
     6901 ctcccgggta accatcaatt cacaggatga aatgggccag ttggccgagg gatttaataa
     6961 tatggtctgt gagcttcaga ggtacatcgc taaatcctat cttgcccaga acaaacagaa
     7021 ggaagcagag ttaaatgcgt taaagacaca aattcaaccg cattttttat acaatacgct
     7081 ggaagtaatc cggatgagtg ccgtagatca cggagatgaa caggtcgccg agatggtcca
     7141 ttcattggct gcccagttac gataccttat cggttgtaac gacgatctgg tcactctcgg
     7201 ccaggagctt gagatggtag agaactattt tcgcttaatc cggatccgtt atgaggacaa
     7261 gattgatttg gagatccgca ttcccgaaca gataaggcat taccatattc tcaaattaac
     7321 cttgcaaccg gtggtggaaa acgcggtagt tcacggtctc aaacccaaaa caagccatgg
     7381 gaaggtgctg atcaccgggg agatcgcagg cggtcatctg gtgctaaacg ttttggatga
     7441 tggaattgga atgaccgagg cgactttggc cggaatcaat gatttattgg ttggggagaa
     7501 gataggcgaa ccgacagccg ggggatggaa aaatgttggt ttgaagaacg tccatgaccg
     7561 gctgcggctg cgttttgggg aaccgtacgg cattgatctc aaaagccagg ccttgattgg
     7621 cacggtggtt cggatcatca ccccgataat cagcgatata aacaactccg gaggtgttaa
     7681 tcatgctcaa gatggtgctg gctgatgatg aacctttgat cctgaaagga ctgaaaaaaa
     7741 tcatcaattg gtccgaatac ggcattgaaa taaccgggga agccgcaaac ggccctcagg
     7801 cggagcaact cctgctctct gaaaagcccg atatcttcct atcagacatc tgcatgcccg
     7861 ggaaaaacgg gatagaactt ttacagatgc ttcatgatcg aaatatcaat acgaaagtga
     7921 ttttactcag cggctatcag gagttttcct acgccaagga agccatcgct ttcggggcgc
     7981 ttgagtatat tttaaaaccc atcgacaagg agcaacttgc cggggctatc cggaaagcga
     8041 gttccctgat tcgtaaggaa agggaggaaa ccggactccg ggagaagttg gcccgttatg
     8101 aaatggagcg caccgataca atcttagaga atgcctggga gcacttgatc gccggtacca
     8161 taagcccacc ggagatcaag gtcttagccc ccgatatcgg agacgggttc tatactgtcg
     8221 taaatattat catcgatgaa ttgtttgata acccccttgc atttgcggcg ggggagattc
     8281 aattgatcca gtattcggta tgcaccctca tccaaaagat gcttgaggag aaaaaagccg
     8341 ggcatgcctt gatgaagggg caacagatta cgctagtcct ttgttatcct gaaaaaaggc
     8401 ctgcccccac cgaagctcag gatttcgccg gcgaggtcgg cagaatggta aagaatcaaa
     8461 tgatgaagac ggttacaatc ggaatcggcg agatggtctc cggtttggaa ttggtacccc
     8521 gttcctacca atccgcactc agggcgtttg aagatcgatt ctttgcggga aacgacagga
     8581 tcattatggc aacacaagag catatcccca aatatcaagt agaagatctc tatcttgagc
     8641 aaaagaagct ctgcaatggt ttattaacca atgaaagaga actgttcgat gaaagcctgg
     8701 caaatataaa atatatcgtt tccgatatcg cctggggcga taaagagacg gccatcggtt
     8761 actatttaac gactctggcg aatttgagcc gtgaactttc cggttacggt attgatctgg
     8821 aaagtcttgc tgataaaacg ggtcgtatct ctaaagaact gaaaggattg gcaactttga
     8881 atgaattgca ctcctggctg accaaatatt tggatgaaat cacgcgccgg attggtttcc
     8941 aaatcaaaag ctcggaaccc caggtgatca agaaagtcaa aaagtatatc gaagatgatt
     9001 ataaccatga tatcaccctg gaggctgtgg cgggaatcgc ctgtatgaac cctaattatt
     9061 tcagcgtttt ctttaagaat cataccggtg agaatttcag ggatttcctg ttgaggattc
     9121 gaatggagaa ggcgttggga atcttgttaa gtaccgaatg taaaacctat gagattgccg
     9181 atcaagtcgg attcaacgac cccaagcatt ttagcgagat gttcaagaag tattacggca
     9241 aaaatcccat ggaatacaag aaggatttct taaaaaaacc gaccttatcc taaagaaact
     9301 cacttcattg tgagtttgtt ttttgttaca gttaagttac agcatgtaaa aaattataag
     9361 gaggaagaaa tcatggcacg aaggggttgg atggggttcg ttctcatcgg tttgattctt
     9421 tcgcttttca gctttaatct gacaatggcg gcgccaaaag agacaaaact gacatgtata
     9481 tacgccactg gagacccttt gaccaaacag ttggttcacg aaacgatcgt caaattcatg
     9541 caacggaatc cgggcattaa aatctccgaa gatctttcaa taaacacggg ggcttatgcc
     9601 gatgccctaa aaatcaagga ctcggtcggt gagttccctg atttcgtgga gatgcgggat
     9661 gcaccgatgt ttgtccgggc ggagaaattg gctcctctgc ccaaagaact gatgggtttg
     9721 tatgaaacca ccatcccgat atacgggact gtatataccg caccgtttat ggcggcagct
     9781 ccgaatggcg tttattataa caaaaccttt tttaagaaaa acggtttaaa agagccgaaa
     9841 acctatgccg agtttttaca actttgtgag aagatcaaag ccaagggtgt atcgccgctg
     9901 gtagccggga tcaaggatat ctggcacatg ggctttttgt tcggccagtt ttgggttacc
     9961 gatatcgcca gtaaaaaccc gaattggatc gcggaccgtt atgcgggaaa agttcatttc
    10021 accgatgcgg attttgccga tgccatgaaa cagttggtga cgcttttcac caaagggtat
    10081 gtggaaccgg gttacatgag cacaaccgag gcgcaatgcc cctcgatttt agtcagtggg
    10141 aaagtggcca tgtattactg tgggcctcat gtgatcaaac agatcacgga tgcggatccc
    10201 agtttcgacc tgggctggtt ccccatcccg gatgccaagg gaaaagtcta cctgcttggt
    10261 ggagcaacga aagacggttg ggctatatcc gcgcaggccg ctaaagatcc cgcgaaggtc
    10321 caggctttcg tgaaattcgt caagtttttc atggcgaaac cccaatactc caaattcgtc
    10381 gcttcgggaa actatttccc cacctcgaaa caggccatca catacaaagc gactccggcc
    10441 atgcaatccg ttttaaaagc ttatgccaag gccggcaaga gtctaaattg gaatcagggc
    10501 gttggcgcca atgagatccc gccggccttc cggaactgga cttacaagaa agtccaggag
    10561 atgctgatcg gcacggtcag cgtggaagac ggtttaaagc agatggatga ggagtttgac
    10621 aaagaggcca gggattttaa tcccaccaaa ttcgtaccgg agaaattatc ggtaccaaca
    10681 gccacgaaat aagcatggca cagaagtaag atggttagtg gagcctcccg tagttgacgg
    10741 gaggctctaa attaaagagg agagttgtta tggctaaaaa gaggcgtctg ataagccagg
    10801 tttcttatgg aatgctttta cctgcctttt tactatacac gggatttgtc attgttccgg
    10861 ccttatccag tttttattac agtttgacat catgggacgg cttgagcaat atgatgccgt
    10921 ttatcggtct acggaattat atggatatgt ttcatgatat ccggtttttt acagccgtta
    10981 aaaatacgat gttcattgcg atagctgcta cgatcctgga aaatgcggtc gcgttgggcc
    11041 tggcactact ggtggacagg atcaaaatat tcaagagttt cttccggagc gtattttata
    11101 ttccggtgct tttgagcggg attatcaccg gatttatctg gtcggtgatg atgaattaca
    11161 gtttcggtgt gatcaatcag ttcttcgcta tgattcacct tgatatcttg agacttaact
    11221 ggcttggcga tccgaaaatc gccttgatct cgatcctcct caccatgatc tggaaaggcg
    11281 cagggtacta tatgatcatt tatctggcgg ggcttcaagg gataccccag gagatactgg
    11341 aagcctccag gatcgatggg gccagcggcc ttcaacagtt ccggtttatt acgtttccga
    11401 tgcttgccgg ggcggttaca atcaatatga cgctttcact catcggcggt ttaaaggtat
    11461 ttgatcaggt cgcagtgatg actgacggcg gcccggggtt tgccaccgag accattgctt
    11521 atcaaatttt caaagtcgct tttgccgatt caaaacaagg ctacggaact gccgtggccg
    11581 tcatgctttt tgcgattacc ctggttttct cggcgatcca attggctctc ctaaggaagc
    11641 gggaggtgga attataaatg ggaactacta tcgatctaaa acagcctttc gatgaaaaaa
    11701 ggatcggaaa aacacatgat attctcctgg tcatcctggt tgcgtttttg gcggcactgt
    11761 tcttttatcc cgtatatttc agtatcattt cggcctttaa aacccagggc gagatcatga
    11821 gggatgcgat ggcgtttcct tccgccctgc gcttagataa ttttatttat ctgtggaacg
    11881 aaacccggtt tccccaggcg gttgtcatga gcgccttttt gaccgtcact tcgacatgtt
    11941 tgatgatttt ggtgattccc atggctgcct atgtgattga acggcgaaat tccaagctaa
    12001 ccggcctgat ttatatcttt ttcatcagcg ggatgatgat tccgttccag gcctatatga
    12061 tcccgctttt ccggcagctc aagtggctgc atcttttcgg cacggtttgg ggcccgatcg
    12121 ttgtttatat ctccggcgcc accgccttcg gcaccttact ctttaccagt ttcatcaagg
    12181 gtatcccccg ggagattgag gagtcggcgg ccatcgacgg ttgcagcagt ttcaatacat
    12241 tctggcaaat cgtctttccc ctgctgaagc cgtgcaccgc cagcatggtt attttacagg
    12301 gcctggggat ctggaacgac tttttaatgc ccttgctggt gctcccctcg gaccaggcga
    12361 agaccatcaa tgtggagatc tattctttcg tcagcgaact ggcgacccgc tgggatgtac
    12421 tgttcgccgg cagtttctgt tcaatgatcc cgattattct gatctttggc ttcttgcaga
    12481 aatactttgt caagggaatc accgccggga ccgtgaaggg ttaaggcttt gaaatgaggg
    12541 aatgaaaaaa atgggacaga tcataaatcc gatactgagg ggctttaacc ccgacccgtc
    12601 cattatccgg gtcggagatg attattatat tgccacatcg acatttgaat ggtttcccgg
    12661 cgtacagatc cattattcca aagacctgat ccattggaaa ccggtggcgc atccgctcaa
    12721 ccggttatcc caattaaaca tggcgggtat tgattcttcc tgcggggtat gggctcccaa
    12781 tctatcctat gatcggggaa ccttttacct tatttacacc atcgtcagga gcacgggcgg
    12841 aatttttaag gatacgctca actttctggt gactgccgcg gatattcagg gagagtggtc
    12901 ggagccgata tttctcaatg ccagcgggtt tgacccttcg ctatttcatg acgacgacgg
    12961 ccggaaatgg ctggtgaata tggactggaa tcacggccag ggaaagaagc ggttcggagg
    13021 gatcctcctc caggaatacg cccctgtcca aaagcggctc gttggtccgg tccaaaacat
    13081 ctttcaacat tcgggtttgg ccgaaggacc gaatctttac aagcgggatg attactacta
    13141 tctcatgttg gcggagggcg ggacgggatt gaaccattcc gtatggatgg cccgttccaa
    13201 gtcaatcgcc ggcccctacg agactgatct caagaacccc atccttactg caagggaaga
    13261 cccaaggatt cccttgcaga aggcgggtca cgccagtctg gttcatactc catccgggga
    13321 gtggtatatg gctcatctct gcggcaggcc tattccggcc tcgggaaggt gcacactggg
    13381 aagggaaacc tgcatccaaa aggtaatttg gagcgatgac gggtggttaa ggctggctca
    13441 gggaggaaac aaacctttga tgacggtacc cggaccggat ctgcctgaat atccttttcc
    13501 ccaggaatca gttaaggatg attttgactc agaaaaactg ggcatccact tccagacact
    13561 cagagtccct cttggagagg aatacctgtc cctgatagaa cgcccgggtt atctgcgttt
    13621 gaagggcgga gaatccttga actcaaggta ttttcaaagc ctgatcgccc gaagacagca
    13681 ggctttttgt tatacggcaa ccgcctgtat ggaattcgaa cccgagtcgt acaagcagat
    13741 ggcgggctta atatgtatat atgacattca taactatttt tacctgcggg tcagccatga
    13801 cggcacagga aaatgcctcg ccatcatcaa ttgcgataat gggacataca actatccatt
    13861 ggaaaaagat atttatatcg gcggggattc gcgacattat cttcaggcga gggtcgatta
    13921 tgaccggtta cggttttact actcaagtga cggagaacag tggctgcaag taggcggcaa
    13981 ctttgactac agcaggatat cggatgaagg aatcaacaaa ctggccttca ccggagccat
    14041 gatcggcatc tgctgccagg atctgtcggg aatgcgaaaa catgcggatt ttgattattt
    14101 cgaatatatc gagagagatt ttgcagggta aagcccctca gccaacattt gggtctcgcg
    14161 tacacggtac gtgcgccccc cataacgcta tcataaagcg atcgctacgg aggagggcca
    14221 ttgacttgaa tcgacctgat gggtgagggc atctggaagc cattatgata acatttttat
    14281 tccgagtaca aggtggaggt ctgctaatgt ttaatcaaaa ggaagctttg gagcgcgtgc
    14341 tgcggaagtt ggaaaggacc agtagcagaa tacaagatga aattccctac cggaccagcg
    14401 gaggtaaata cgatgactat tccgccaaga tagactggtg gaccaacgga ttttggccgg
    14461 gtattttatg gctggcatac cgccaaactc aaaaacggga atatgcggat tgggcgttaa
    14521 gggttgagga aaaaatggat gccgcttttc gcgaattcta tcacattcac catgacgcgg
    14581 gtttcatgtg gcatatttcc gcggttgccg attataaatt gaccgggaat ccggcatcgg
    14641 ccaaacgcgg ttatttggcg gccagcgtgc ttgcttcgcg gtttaacccg gcgggctctt
    14701 ttatcagagc ctggaatgac gactgcacag gctgggccat catcgattgt atgatgaatc
    14761 tggccatttt atactgggcg tcgggctata tccatgatcc gaggttttcc aatatcgcgc
    14821 tggcgcatgc ggcaacggcg caaaagtatt tcatccgcga ggatggatcg gccagacata
    14881 tctgtgtatt tgaccccgtt tccggagaat acctggagaa tttgggaggc caggggtacg
    14941 gggtggattc cgcgtggagc cgcggcgcgg cctgggccct ctatgggttt gcgatgagcg
    15001 ccaaatatag cggcagaccg gatttcctga atacggccaa acgggtggca aacttttttg
    15061 tagcccatct tccggaagac tcggttccct ttgccgattt taaggcgcca gcggatatca
    15121 atattcataa ggattccagt gccgcagcga tagcggccgg cgggctattg cttctctcaa
    15181 ggcttgtccc cgaagacgaa aaaccatttt accgggattc cgccgtgaag atcgtttctt
    15241 ctctatatga taattataca gactgggatg gagatgaggc gctgattcaa aaaggatgca
    15301 cggcatttca ttcaaagccc gaggaaggtc tttcgacatc attgatttac ggcgattatt
    15361 tcttccttga agcattgatg cagctcaatg gttttgaagg gctgttttag gcggggtgat
    15421 cgtcaatcat tcgtcttatt cccatgatct ttcgtcaacg ttgtttgatt cagggactat
    15481 tgatttatgg gaatccgcaa ataaaccgat aatcccggtt caagcggtat taaggtaatg
    15541 ccgtatgcgt tcccgtatgc gtatttaagc cggtgatgga tattggagat tcccacgccg
    15601 ttgccttggc ttccggattc gtaagtttcc aactccaaac ttttattgat ctcccggatg
    15661 cgctccatgg tcatgcccgg accattatcg gaaacggata tttcgaggtc attctcgctt
    15721 ttattcgctt gtaccctgat taaaaccggg tccaggcttt tttctacccc atggatgaag
    15781 cagttttcaa ccaacggttg aatacagaat ttgaatatct ttgtttttaa gagttcctcc
    15841 ggaacggaaa gctcaaatga aaaccgggac ccatagcgga tttgttgtaa cgagatgtat
    15901 tctttgagat aatccagttc ttcttgtaaa gtaaccaatt cagccatttt ccgggtggta
    15961 tagcgaaaaa catgaatgag cgagcgaatc atattaacca atgaagtatt accggttgag
    16021 acggccaaac ccaccaaaca gtcgagggtg ttaaataaga agtggggatt aatatgggct
    16081 tgaagcgcgc ggagttcggc cgaacgggcc agttgctcga cttggcgcaa ccgcttggta
    16141 ttggcgtaga tagtagctat gagaattgta gcaatcagta aaagcaaagc ggtaatgatc
    16201 agggggcctg aagcaatccg gcgtaccggg tcgactactt cggctgccgg gtactcaacg
    16261 gttagaacca gacccagtaa gggatctttt aaatagactc cggccatttt ccggtcattt
    16321 tttttgtagg tcccaaatcc atgcacttcg ttgggatatg acagcatttg gcgaatgagg
    16381 ggatggtcgc tgattttatt tagtaaattc aaatttccgc tgtaatagcg ataagggcat
    16441 gccaccaata ttccatccgt ggtataaatc gatacgggta tggttgacca atcaatatca
    16501 acaccggtat tttcagttaa cgcgatattt aggtcaagct ctgctccggc cgctcgaatg
    16561 atttggattt catgtaaatc aaagcttaat aagggcacca gagcggtgaa atttgactga
    16621 tcaataaagg gttttccaac caaagaccgg ccgcgtccga tttgaaatcc agtaaaattt
    16681 tggggagttt ttccggagag gtactcatgc caccatggta agttttttac ataatcgggt
    16741 cgggtatttg gagagggaac caggattcca ccggggtaag ccacccaact cctgattaca
    16801 ctaggagttt gggtgcgaat ggtatccatt tcattttgta acaaggtacg gtccgccgtg
    16861 gttgcaacgg ccgaacggac caggttatct aatttcagtg cggcttgtaa aaatacttca
    16921 tccagttgtt gacggtaaaa attggcctcc cacaataaac gttgctccat attggttttc
    16981 aactggttat tcacttgaag atataccagg aaactgctgg caacggtagc aacgataatt
    17041 agcgcgcaaa tggcaagcca aattttataa aaagtacttt tggtataaga aaatatcggc
    17101 tgtttcgtca cggtaagccc ccgagtgatt gcgaacgcgc gcgcgactca ttttttgaaa
    17161 ttcgagcgat aattgctggg agtaaaaccg gtttcccgcc ggaaaaccct gctgaaatag
    17221 gcaatgtcag tgtaaccgac tttggttgcg acttcctgaa tttgtaaaat ggaatgagtt
    17281 aataattctt ccgctttgtt gatccggtag taggtcagat actccagaaa gttttgcccg
    17341 caatatttgg caaatcgtga actcaaatat accgggttta ccgagacaaa tttggctacc
    17401 tggattaagg tcaatggttc cgcgtaatga tccttgatat attctttggc ttgttcaatg
    17461 taagcaggta tacagccttt ctccgcgata gttaaacttt ccccctgcag ctccgcttga
    17521 atcttgctac gcatttgggc ctttttcgtc tgatcgcgct ctaaaagttc cttggtacgt
    17581 ttgagtaacg cggctacatc ttcgggaaaa accggtttca acatatattc aaacacattc
    17641 aggcggatgg cctgttgggc ataggcaaaa tcatcatatc cggtgataat tgccgcataa
    17701 ccgtcccaac cgacttggcg catctggcgg agtaggctca atccatccgt aacaggcatt
    17761 ttgatatcgg tgatcaccag atcgggtttg gtcattccaa ggaaataaag agcgtcctct
    17821 gctcccagtg ccttggcgat aacatggaaa tctaaatccg cctgatggat ataattcgtg
    17881 agtttttcca gggcactcga ttcatcatca acaaccatta cttgaaacag gccaacccct
    17941 cccttcaaag cattcattca tcaataaaat tttatcataa cgaattgcac ttttacacag
    18001 gatgctaaaa tagtccaaaa aatgcttaat aatgtctatc ccgatggatt gttaaggcta
    18061 agtataatat aaatgacgct tttgttacga acgttccaaa ggtgtcacaa aaagaagggg
    18121 gagatcatat gaagaagtgg tttcgtttaa cagggtgtct ggtcttgtta atcgcgtttt
    18181 tttcaacctt ggcctccgca gagaacacag tgacgattca atatgcattc tggggcaacc
    18241 ctaccgcaat cggagtagaa aaagatatca tcgacgaatt cgaaaaaact caccccaata
    18301 tcaaggttac tccggtagta gcagcttata acgattatca tacaaaatta cttacaatgt
    18361 atgccggggg acaggcgccg gatgtcatga gaatcgactc ctattacatg gccgatttca
    18421 tgaagaataa agccttaaaa gatattaccg ggctaatcaa acaggataag ctgaatatga
    18481 attcatatta tcccgccgga ttgctcgact gcatgcaagg caacaggtat tacgggttac
    18541 cctgggggac tgccccggtt ttcatgttca tcaatgtgaa aatgtttaag gatacgggta
    18601 ttcccgtgcc atccgtcgat tggaaatatg aagactttct taaaatagcc agacaattga
    18661 gcaagggtga aggcatggat cgccaatacg gttttgctgt cggaactact gatttgacga
    18721 acctgtttcc atttatttgg gccggcggcg gcgatctttt tgataaaaac cggagaaagt
    18781 tcactctaaa taaaccgcag gccgttcaaa agatccagga aattgccaat atgattaaag
    18841 aaggcatcat ccctgatccg gcccagttta catccggaga tgtctttaac cggtggtctg
    18901 ccaattataa actggccatg cgtatgggta cggctgcaga aattctctcg atgcaaagta
    18961 tagccgggta tgagtttgaa gtattgccgt tcccgggcac tgtgaaatac ccccggacaa
    19021 ccatctccaa atcaaatgtg gtaggcatca gttctacgac caaacaagcg aaggccgcct
    19081 gggaattctt gaaattttta aggggaccgg gacaacccgg agaagtcttg tatatgaagg
    19141 cgaaacgggt tcccccttct tttgatgatc ccgagttatg gaaactctac gccgatcctg
    19201 ccaaatcacc gaaaatggtg gtcgagtcat cacaggcaat tgccgtaaaa tatggccatg
    19261 ttttaccgtt acgaaccggg tggattgaaa ttcaaagttt gttgcttcca gagttgcaac
    19321 gagtatatgc aggacaaatc actgccgaga aggccatgaa cgagatcgct cccaaaattc
    19381 aggaagtgat ggatcgtacc ggcaaataaa cgggggttcc gccagaaggg gcagcaatgc
    19441 cccttctcaa tttatcggat ttgatctctg gcaagttccg cttccactga aacaatcaac
    19501 acatccatga gaagtcatgg gcagttaatc aaggaggcat tggtatgaaa aggcaatcaa
    19561 cccgatttcg ggaggcggtg gtgggttact tattcctgac ccctaatttt ttgggttttg
    19621 taatttttac tattttcccc gtgatcggtt cgttattaat cagtttttcc gattggaact
    19681 tgattacttc gcctgcagca gtgggattaa ccaattacgc caatctcttt catgaccaag
    19741 ttttcatgca atcattgacc aataccgtca tttactcatt tgtcagcgtc atgctgaata
    19801 tcatcttagc tctctttttg gcaatattac tcaataagaa aattcatggg ctcagttttt
    19861 ttcgtgcggc ctatttcctt cctgtagttt attcatcggt agcagtggca ttaatctggc
    19921 aatggctgtt cgattatcaa atgggcttac ttaaccattt cttatcttgg tttcagttgg
    19981 gtccctttcc gtggataaat tcaccgcaat gggctttgcc aagtgtgatt ttggtatcgg
    20041 tttggaaggg tatgggttac aatatggtga tttttcttgc agcattacag ggtgtacccg
    20101 gtgaattata tgaagttgca aaaatcgatg gcgccaatgc ctggaaaacc ttttggaatg
    20161 taacttggcc gatgatttca ccatccacat tctttgtcac ggtgacttca atcataaatt
    20221 ccttccaagt tttcgacctg acaacggttc taaccaacgg cggtcccgca aatgcaacca
    20281 atacattggt aatgctggtt tttcagcacg ccttccgctt tttcaggatg ggttatgctt
    20341 ccgccatagc ttatactctg tttgggatcg ttttcatttt tacaattctc caaactgtgg
    20401 tggcaaaacg ctgggtccat tattaggcga accgcggatc ggaatatatg gaggtgtcaa
    20461 ctgtgaaaac tagcggttta atgatagaaa aaccccataa ttattttgat accaaatttc
    20521 tgtggcaact gacggtttat ttggtgctgg taatcggagc gcttatggta ctgttcccgt
    20581 tggtttgggc gctcattgct tcagtcaagt catatactga gatattcaat aatccgttgg
    20641 gtttaccaag ggaaattgtc tggcaaaact ttattcaggt ttttgaagcg gtcccctttc
    20701 acctttattt tcttaatacc ttgaaaatca ctgccgcttg tacattgggg actgtattta
    20761 cctcagccat cgcggcgtat gcctttgcca gacttcgttt tccggggcgt gattttcttt
    20821 ttatgggtta tctggctacc ttgatgattc cccgtcaaat tactctggtg ccgacattta
    20881 ttatcatgag atggcttggt ttgcttgaca atcatttatc cttgatcttg cccggattat
    20941 tcagcgctta tggtactttt ttattacgcc agttcttttt gaccattccg gttgagctgg
    21001 aggaagccgc tattatcgat ggttgcggat atctgcgtag atttgcccgg attattatgc
    21061 ctttaagcaa gccggcctta gcaactttag gcatcttcac actcatgggc acgtggaatg
    21121 attatcttta cccgatggtc tttttaaata gtgagaacaa tcgtactttg gtgatggggc
    21181 tggcaatttt tcgcggcgat gtggatgtcc agtggaacct cttgatggct gcagttgtaa
    21241 tgacaagcgc cccaatcgtc atcgctttca tcttggcgca acgatttttt gtcgaaggta
    21301 ttgctacgac tggtcttaag gggtagagct cagattgaaa atcaccggca tatgaattat
    21361 catataacat gaagattgat aatgatgctt ctcatgaaga ggcaaggaga tactcgtgga
    21421 tatggtcaaa aagattgctt ttgtgatgga caaagatacc cttttttatc tgcccgaggt
    21481 gttgacgccc ccggtgggac acgcggtccg tattttattc cgggatttcg agaaggtctt
    21541 gggatccgcc ccaaaacaat gtgatgatat tcattccgct tcagtgatta ttggttatgc
    21601 ttcggaggat gatcccattc ataaccggcc cgaagcgttt gccatccgtt tcgaaaacat
    21661 ggatagtgaa ggatctccgg tgatgcatat ctgcggcagt gatgatttgg gtatcatcta
    21721 tggactgctc tatatcagcc gggaatatct gggggtcgaa ccgttttggt tttgggccga
    21781 taaagaacca aaaaagttgg agcggataca gataccaata agggaataca tttcacccga
    21841 accccgggtc cgctaccggg gctggtttgt caatgatgaa gtatgcctca tcgggtggac
    21901 cgatagctat ccgccgcccc aggaggtatg gcttcccgta tttgaggctt tactgaggtg
    21961 tggtggaaat atggtgatac ccggtaccga tctgccgcgc aacggtatcc attgggagct
    22021 ggcttccgaa atggggttat acatcaccca gcatcacgcc gaacccctcg gggcggagat
    22081 gttcttcagg gcttatcccc atgaagaacc tagctacgat aagaaccggg tattgtttga
    22141 gggtttatgg gaagatgccg ttcgtaagaa ccgggataaa aaagtaattt gggcattggg
    22201 tttccgggga cagggagact gtccgttctg ggaacaggat ccgacctatt cgaccccgga
    22261 acgccggggt gagctcatca gtaaggccat ccgcaggcaa tacgagttgg tcagtcaata
    22321 tgtcgagaat ccggcatgcg cgacgtatct ctatggcgag atcaccgagc tttaccgggc
    22381 aggcttgctg gatttacccg atggcattat caagatttgg gccgataacg gttatggcaa
    22441 gatggtttcc agaagacaag gcaaccataa ccccagagtt ccctcactcc cggcaccgac
    22501 agaaaccgga ccgcacggcc tgtattacca tatcaccttt catgatcttc aggcctccaa
    22561 ccacctgacc atgtttccat ccagccccgg tctggtgaag gatgaattgt taaaagcctt
    22621 tcgggcgggg gcggatcgtt atttattgct caacagcggg aatatcaaac cgcacctcta
    22681 tttgctggag atcgttagcc gcctctggaa tgaaggaacc atcgacattg atgagcattt
    22741 ggacgacttc tgcgggaggt ttttcagtac cgcccggggg aaagcaagag aatgttatca
    22801 agattatttt catcagacga ttcaatatgg gatctacacc gacgataaag ccggtgaaga
    22861 gttttaccat catcccccca ggtccatcat tggccactgg ataaggaacg agatcaccga
    22921 aactgcttgt gatttacaat gggctaccgg tgaggtgacc tttgacgaac aagtacgatg
    22981 tttctttgaa aaactaattg ccgcccggga aggttggcgg aaattgcaag agaaggcccg
    23041 gacgaccgcg gcgatgcttc gtaaagagga agccgtattt ttcagggatc atctggcatt
    23101 ccaagtagaa ctccattcct cgggatgcaa ggggtttatt gccttatgtc aaagttattt
    23161 tgccttccgg gaaggcgatt ttccccgggc gtttgtctac gcctcccagt ccttatggaa
    23221 ttataatgat agtataaaag ccatggctga agcggaacac gacaggtggc gggattttta
    23281 ccgggccgac tggttaacca atgtgaaaag caccatatat agcttggaga cattgcgaag
    23341 gtatctgagg atgcatgggg acagtcccga atttttcttg tggaacaaag cctatctgat
    23401 gccggagagc gagaagaaga tctacctgga aaacactcat cgcaaaccgt tgtccgatga
    23461 tgatttggca aggaaattaa aggataaatt cggcatcccg gacgagccgc aatgaagtga
    23521 aaggtctttt tcattcacaa gttttaaggg aagaggagat tcaaatgaat cgttttatca
    23581 aatggcgttg tcagtgcatc gccaaagaaa tggttgaaat actgaaacaa aaagcctatg
    23641 atgcgcatta cgctgagaac cttgaggaag ccaggaatat agtgcttgaa atgattcctc
    23701 acggttccag cgtggcggtt ggaggttcag agacattggc agccatgggg cttgtggacg
    23761 tctttcgtaa cggggattac aagttctttg atcgttacca gaacctcccg tttcccgaga
    23821 tcgtggagat ctaccgtcag tccatgcttg ccgatttcct ggtcaccggg accaatgcca
    23881 taacccgaca ggggcaattg gtcaatgtgg acagctccgg gaatcgggtg gcgggtatca
    23941 tcttcggacc gcggcgggtg atcgtggtgg ccggagccaa taaagttgtg gccgatctgg
    24001 acgcggcctt caagcgcctc caagagatcg ccccgatgaa tgccctgcgt aacggccacc
    24061 aaaccccctg cgtcaaaacg ggccggtgcg aggattgtca gatcccggcc cggatgtgca
    24121 actccatcgg catcgtcaac cacgggatga agtttgaggg ccggatctcc gtgatcatgg
    24181 tggcggagga ggtcggattt taggcttcag actcattaag cttttggccg gaatgaaatg
    24241 ccggcccgga atcgaccttt cacactattg agtgcctaaa ggcgactcac ccccttcccc
    24301 ctctcttttt cggcttaaca ttacccttaa gttggaggaa cttgaataaa ttagatcaat
    24361 tcagtaaaat cgatcatttt gatcacatcg atcaactagt aataaacaga aattga
//
LOCUS       DASVMB010000032         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_3470,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000032 DASVMB010000000
VERSION     DASVMB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3470"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /gene="frr"
                     /locus_tag="VF531_01065"
     CDS             complement(<1..322)
                     /gene="frr"
                     /locus_tag="VF531_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7712583.1"
                     /translation="MKTAVEASKKAFSAIRTGRANPSLLDRVTIEYYGNPTPLNQVAS
                     INSPDPRMLVVQPWDKSVLGTIEKAIQKADLGLNPVNDGSVIRLPVPQLTEERRKELV
                     KMVKK"
     gene            complement(364..1089)
                     /gene="pyrH"
                     /locus_tag="VF531_01070"
     CDS             complement(364..1089)
                     /gene="pyrH"
                     /locus_tag="VF531_01070"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7712584.1"
                     /translation="MAAPKYKRIVLKLSGEALAGDKGFGINQDVVNSIAEQTKEIVDM
                     GVQVAVVVGGGNFWRGAAGSLRTGLDRSTSDYMGMLATVINSLALQDSFEALGIATRV
                     QTAIEMRQIAEPYIKRRAVRHLEKGRVVIFGAGTGNPYFSTDTTAALRAAEIEAEIIL
                     MAKKVDGVYSADPKTDPLAIRFEDLKFMEVLQQRLKVMDSTATSLCMENHIPIVVFNL
                     NQAGNIKRIVLGEKVGTFVRGDD"
     gene            complement(1260..2192)
                     /gene="tsf"
                     /locus_tag="VF531_01075"
     CDS             complement(1260..2192)
                     /gene="tsf"
                     /locus_tag="VF531_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7712585.1"
                     /translation="MAEISAAVVKELRERTGAGMMDCKKALGETNGDMDKAIEYLREK
                     GLAAAAKKAGRIAAEGLVSSYIAPDKKVGVLVEVNCETDFVAKNTDFQNFVTDVAQGV
                     AAKDPKDLDSLSKLQLKSGKTVAETGTGLVATIGENMSVRRFVRFENKGTGMVDSYIH
                     MGGKIGVLLDCSTSKADTLTNPEFLTLFKDLAMQVAAARPEYLNRTEVPADVLDREKE
                     IYKAQAMNEGKPANIAEKITIGRLEKFYKEVCLLEQEFIKDNDKTITKLLQEYNAKLG
                     ENIVINRFARFEKGEGIEKRKDDFANEVMSQIKS"
     gene            complement(2392..3159)
                     /gene="rpsB"
                     /locus_tag="VF531_01080"
     CDS             complement(2392..3159)
                     /gene="rpsB"
                     /locus_tag="VF531_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7712586.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKPYIFTERNGIYIIDLQ
                     KTVKKVEEAYNFARSVVENGEAILFVGTKKQAQDAIKEEAERSGMFFVSQRWLGGTLT
                     NFKTIRSRIDRLRKIEQMEQDGSFDALPKKEVLELQKERLRLEKFLGGIRNMHKLPGA
                     VFIVDPRKERIAVAEARKLEIPIIGIVDTNCDPDEIDYIIPGNDDAIRAVKLLAGKIA
                     DAVLEAKQGEQTNDAPATATETVEEQEPSQPELAQEG"
BASE COUNT          722 a          922 c          793 g          912 t
ORIGIN      
        1 cttttttaac catcttcacc aattctttgc ggcgctcttc ggtgagttgt ggcaccggga
       61 gtctgatgac ggaaccgtca tttaccggat tgagtcccaa atccgctttt tgaatggcct
      121 tttcaatggt gccgaggaca gatttatccc aaggttggac caccagcatg cgcgggtcgg
      181 gagaattgat gctggctact tgattgagcg gggttggatt accataatac tcgatagtaa
      241 cccgatccag cagactggga ttagcccgcc cggtgcgaat ggcggaaaaa gcttttttac
      301 tagcttcgac agccgttttc atatgatttt cggtttcctg caaaacttcc ttcaccatag
      361 gatctagtca tcccctctca caaaggttcc tactttctcg ccaaggacga ttcgtttgat
      421 atttccagcc tgattcaggt tgaaaaccac gattggaata tgattctcca tgcacaaaga
      481 tgtggcggtg gagtccatca cttttagcct ttgttgcaaa acttccatga atttcagatc
      541 ctcgaaacgg atcgcaagcg gatcggtttt cggatcggcg ctgtaaaccc catcaacctt
      601 cttggccatc aggatgatct ccgcctcgat ctcggcggct cttaaggcgg ctgtggtatc
      661 cgttgagaaa tacggattgc cggttcccgc gccaaatatt acgacccgcc ccttttccag
      721 atgacggacg gcacgccgct tgatgtaagg ttcggcaatc tgccgcattt cgatggcggt
      781 ttgcacccgg gtggcgattc ccaaggcttc aaaagagtcc tgcagagcca gggaattgat
      841 gactgtggcc aacataccca tataatcgga ggtggaacgg tcgagaccgg tccggaggct
      901 gcctgccgcg ccgcgccaaa aattaccgcc acccaccacc accgccactt gaactcccat
      961 atccacaatt tccttggtct gttcggcaat ggagttaaca acatcctgat tgataccaaa
     1021 tcccttatcg ccggccaagg cttctccgct aagcttgagt acgattcgtt tatatttcgg
     1081 agctgccatc actgcattta cctccgcatt ttcataatat attttcgcga attccaaaat
     1141 taattcctgc agcgggaccc tgactttttt gtaaaaatta acgtccggtt aattacggga
     1201 acttatgcca gagacacccc gggcagaaaa aaaggaacac tcgcgtgttc ccaaaagctt
     1261 cagcttttaa tctgtgacat gacctcgttt gcgaaatcgt ctttccgctt ctcaatgcct
     1321 tcgccttttt caaaacgagc gaaccgattg atgacgatat tctcacccaa tttggcatta
     1381 tactcctgca agagtttggt gatggtttta tcattgtctt tgatgaattc ctgctcaagc
     1441 agacaaactt ccttatagaa cttttccagc cggccaatgg tgatcttctc ggcaatattg
     1501 gcgggtttgc cttcattcat ggcctgagcc ttatagatct ccttctcccg gtcgagcaca
     1561 tccgccggaa cttcggtccg gttcaaatac tcgggccggg ccgcagccac ctgcatcgcc
     1621 agatccttga atagtgtgag aaactcgggg ttagttaagg tatcagcctt gctcgtgctg
     1681 caatcaagca gaactccgat cttgccgccc atgtgaatat aggagtccac catgccggtt
     1741 cctttattct caaaacggac aaaacggcgt accgacatgt tctcaccaat ggtggcaacc
     1801 aggccggtgc cggtctcggc aacggtcttg ccgctcttca gctgtaattt tgagagggaa
     1861 tccagatcct tcggatcttt ggctgccaca ccctgggcga catcggtgac gaaattctga
     1921 aagtccgtat tttttgccac aaaatcggtt tcgcagttca cctcaaccag tacgccgact
     1981 tttttatccg gagcgatata ggaactgacc aacccttcgg cggcaatccg ccccgccttt
     2041 ttggcggccg cggccaatcc tttctcccgt agatattcga tggccttatc catatcccca
     2101 ttggtctcgc ccaaggcctt cttgcaatcc atcattcccg cgccggttct ttcccgcaac
     2161 tctttaacga cagcggcaga tatctcagcc atatgtaata ccctcctatt gattttcttt
     2221 attcatatcc ttcggttctt tgggacgttc gtttggtgac gagcggggcc gcgcccacgc
     2281 gcgatatcta tagtttatga tattttagaa ataattttca cagttttcct atcttcgaaa
     2341 tgaaatcatg aaaaaggggt ggttccggtt gccccggcca cccctttcca atcagccttc
     2401 ttgagctaac tcgggttggg aaggttcctg ttcctctacg gtctcggtag cggtggcggg
     2461 agcgtcgttt gtctgttcac cctgctttgc ttcgagtacc gcgtcggcga tcttcccggc
     2521 cagtaacttg acagcccgga tggcgtcgtc gttaccgggg atgatataat cgatttcatc
     2581 cggatcgcag ttggtatcga cgatcccgat gatcggaatc tcaagctttc gggcttcggc
     2641 caccgcaatc cgctctttgc gcgggtcgac gatgaagacc gctcccggca gtttatgcat
     2701 gttccggatg ccgcccagga atttctccag gcgcagccgt tctttctgaa gttccaacac
     2761 ttcctttttg ggaagggcgt cgaaggaacc gtcctgctcc atttgctcga tcttgcggag
     2821 ccgatcgatc cgcgaacgga tggtcttgaa gttggtcaga gtcccgccca gccaacgttg
     2881 ggatacgaaa aacatccccg aacgttcggc ctcttcttta atggcgtcct gagcctgttt
     2941 cttggttccg acgaagagga tcgcctcgcc gttctcaacg accgagcggg cgaagttgta
     3001 cgcctcctcg accttcttca ccgtcttttg gagatcgatg atgtagatcc cgttccgttc
     3061 ggtgaagatg tacggtttca tcttcggatt ccaacgtctt gtttggtgac caaaatgtac
     3121 gccggcttcc agcaattgct tcatggaaat cacagccaca ttagccaccc cctttcttgg
     3181 ttttttttac cgccgcccgc ctcgtctccg gctcgggcct tacgtgtaag gacccccggc
     3241 caaatccgcg ggcgtgtgtg gtggcgcgtg tgcgcgaata caccggtaaa tagtataaca
     3301 caggaaggaa ggattgaaaa gagaattttt aagtttctaa aaagtaatc
//
LOCUS       DASVMB010000033        12588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_445794,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000033 DASVMB010000000
VERSION     DASVMB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445794"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /gene="glmS"
                     /locus_tag="VF531_01085"
     CDS             complement(<1..1454)
                     /gene="glmS"
                     /locus_tag="VF531_01085"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7712587.1"
                     /translation="MCGIVGYIGKQEAVPVLLAGLKRLEYRGYDSAGVTVYNQGKLNS
                     CKTVGRLSKIEERLAEIKLTGNLGIGHTRWATHGRPSERNAHPHFDCNQKISLVHNGI
                     IENYIGLKEWLQEKGHIFNSETDTEVLPHLIEEYYQGDLAAAVREALKKVEGAFALVV
                     ISDEHPGEIVVARKASPLIIGLGKEENFIASDIPAVLPYTRDIYILEDGEMAVITAEK
                     AEVCDFNGNPRSKEVYHVQWDIAAAEKGGYEDFMLKEIHEQAKAVRDTLAGKVDHVEM
                     KILLPEVKLDPREIDTLEKIQIVACGTAYHAGMVGKYLIEKLARIPVEVDLASEFRYR
                     DPMVSQKSLTIIISQSGETADTLAGLREAKRLGSRVLAITNVVGSSVAREADDLILTL
                     AGPEIAVASTKAYTTQLLSLYLLAIFLAQHRGTLSQNERVGMVEAMGNLPGQIDKVLN
                     TETKIKEFAEDFKGCEDIFFLGRGLDYAVSLEGAL"
     gene            complement(2349..3704)
                     /gene="glmM"
                     /locus_tag="VF531_01090"
     CDS             complement(2349..3704)
                     /gene="glmM"
                     /locus_tag="VF531_01090"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7712588.1"
                     /translation="MARLFGTDGVRGVANENLTPELAFKLGQAAGIYFQRGRGTAEER
                     PRVIIGKDTRISGDMLEAALMSGLTSVGVDVYRLGVLPTPGVAFLCRRLGSVAGVMIS
                     ASHNPVEDNGIKFFDHQGFKLTDEVEDEIEAILRTGLEQFERPTGIKVGQINDAHDAV
                     KQYEDYLVSTVQVRFDGLRVVVDCGFGAAYQLAPQIMQRLGAEVIPLHAMDDGSRINV
                     KCGSTHPEILQREVVAYGANLGIAHDGDADRVIAVDEAGRVVDGDQIITVCGLALLQE
                     GKLRHNKVAVTVYSNLGLSQAFKKHQADVVVTANGDRYVLEALRDQDLVLGGEQSGHI
                     IFLEKNTTGDGILTALQLIAVLVKSGRKLSELAGQMERFPQVLQNVRVAQKEGWEANP
                     RIREAIDQAKQKLGDSGRVFVRASGTEPLIRVMAEGPEEATLRELVGSIAVVIQKEQG
                     T"
     gene            complement(3746..5359)
                     /locus_tag="VF531_01095"
     CDS             complement(3746..5359)
                     /locus_tag="VF531_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7712589.1"
                     /translation="MWLKLAQLRVPLNVTVAQLPAWVARRLGLEPRTVLEVRIDRKSL
                     DARHKSPVFIYNLYLRIDLAPQKQDAFLKQHAELDLTVTGPPAVVPLQVGKTKPSISF
                     RPVIVGAGPAGLFAGLKLAAAGFKPLIIERGEGLGERIARVDHFWHTGELDPESNIQY
                     GCGGAGTFSDGKLTTRIKDPRLAEILQVLIELGAPEDIRYLQLPHIGTDLLREVVTKL
                     ERLIGEYGGEIQFRNRLTDLRIDRGEIRGVSINSGTHLETGLVLLAVGNSARDLYALL
                     SEKGLKIEAKPIAVGLRIEHPQDLIDRAQYGSWAGNLQLGPGDYRLTYQDRESRRGVY
                     TFCMCPGGQVVGAASEPGGVVTNGMSYHARNSGVANAAIVVTIPASDYGDTGPLGGID
                     FQRRLERKAYEAGGGKFFAPAQRVADFLKCEPSQGFIALKPSYLPGTRPANLWEVLPP
                     NLCQAIAAAIRQFGRELKGFDWPDAVLTGVETRTSAPVRIVRDVEREAEGFNGIYPVG
                     EGAGYAGGIISSALDGWKTAEVICEKYCK"
     gene            complement(5479..6291)
                     /gene="cdaA"
                     /locus_tag="VF531_01100"
     CDS             complement(5479..6291)
                     /gene="cdaA"
                     /locus_tag="VF531_01100"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX7712590.1"
                     /translation="MLQFLIEHNIIRTAIDLFLVILIITQLFKLMRGTKGVFFLNGII
                     ILFLTYIFSRALGLEYLNLLLSWIIIMLGVALPVIFQNELKRAMEILGRKNPIIKWFV
                     KPPVIAIESIDIVAEAVESLAKQKIGALIVLEREDPLPVVSESGSYIDAALSAILIEE
                     IFYPGSPLHDGAILIKGNRIRAAGCFLPLDNQLPLPQELGSRHRAGFSLASQSDALVV
                     IVSEETGGISLAYNGVLDTGYSSERLKGTLKELIQPMQDKPATTVSGKAEVK"
     gene            complement(6473..7786)
                     /locus_tag="VF531_01105"
     CDS             complement(6473..7786)
                     /locus_tag="VF531_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX7712591.1"
                     /translation="MFERLFKFQENKTTFGREVMAGCVTFMTMAYIIFVNPSILGDAM
                     GKGMVPALIFATCLAAGITTICMGLFTNYPLALASGMGLNALLAYGVVLGMKVPWQTA
                     MGIVVIEGLIITILVLTNFREWVMDAIPLDLKRAIGVGIGLFIALIGLKDANLVVADP
                     VTLITFGKLTAQSLIALAGIFIIAVLMGKQVKGSILIGIAFSTILAVLAGLVDLPKGS
                     WVTGIRPEYFQTFFALDLKGALSWGLAATIFAFLITDFFDTMGTVFAIGEEAGFVGKD
                     GKLPRLKKVLLVDSLAAVVGGLFGCSSVTTYVESASGVGEGGRTGLTSVVTGLLFLLA
                     IFFTPLVGIVPTYAVAPALIIVGFLMLTAVKHMHWDDFSTAFPAFLTMVMIPFTYNIS
                     KGIGYGFISYVLIKVVSGKHKEVHPLMYLVSAAFVLDFFLTSIGK"
     gene            complement(8076..8633)
                     /gene="hpt"
                     /locus_tag="VF531_01110"
     CDS             complement(8076..8633)
                     /gene="hpt"
                     /locus_tag="VF531_01110"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX7712592.1"
                     /translation="MILDADLKEVLYRPEEISARVRELGQMITNDYKGKDLLLVGILK
                     GAVPFVADLIRQIDLPLAYDLMAVSSYGASTKSSGTVRILKDLDAAIEGRDVIIVEDI
                     VDTGLTLQYLLENLKARRLNSIKLCTLLDKPDRRKVAIQPDYNGFTIPDVFVVGYGLD
                     YAEKYRNLPYIGVLKEQVYRHPSGE"
     gene            complement(8734..9228)
                     /gene="ilvN"
                     /locus_tag="VF531_01115"
     CDS             complement(8734..9228)
                     /gene="ilvN"
                     /locus_tag="VF531_01115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7712593.1"
                     /translation="MRHIISALVVNRSGVMVRVAGLFSRRGYNIESIAVGGSEDPELS
                     RMVIEVEAEDEQVLEQIEKQLYKLIDVVKVNDLPVKETVGRELALIKVNAGSQNRSEI
                     LQIVDIFRAKIIDVSSRAVIVEITGDSEKIEAIIALLKPFGIREIVRTGQIAMLRTPK
                     KGNI"
     gene            complement(9228..10895)
                     /gene="ilvB"
                     /locus_tag="VF531_01120"
     CDS             complement(9228..10895)
                     /gene="ilvB"
                     /locus_tag="VF531_01120"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX7712594.1"
                     /translation="MKNSGAEILLDCLAREGVEVIFGYPGGQVIPIYDALYDAPLRHI
                     LVRHEQGAVHAADGYARSTGKTGVCLATSGPGATNLVTGIANAYMDSVPLVAITGQVP
                     TFLIGYDSFQEADITGITIPITKHNYLVKRIEDIPRVVKEAFHIAATGRPGPVLIDLA
                     KDVTQAKAKLEYPETVELPGYKPNYIGNARQIKEALEAIGEAKRPLLYIGGGVIAAGA
                     SAELIQFAERLDIPVTATLMGLTAFPSGHPLYLGMLGMHGTAAANFAVGTTDLLIAIG
                     ARFDDRVTGKVDSFAPQARIIHIDIDPAEIGKNVRVDIPIVGDAKIVLETLNEKAGTG
                     KHPEWRDQIGQWQKEYPLHYEPTGFKPQQVIETLSELTRGEAIICTEVGQNQMWTAQF
                     FRFQKPRSLITSGGLGTMGFGFPAAIGAQVGNPDRMVIDIAGDGSFQMTIQELGTAVA
                     HRIPVKVVILNNFYLGMVRQWQEFFQNKRYSGTVLDSNPDFVKIAEAYGAKGYRITEP
                     NQVRETLAAALSEPGPVVVDCWVDREENVLPMVPANSPLNRMIGVNL"
     gene            complement(11462..>12588)
                     /locus_tag="VF531_01125"
     CDS             complement(11462..>12588)
                     /locus_tag="VF531_01125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7712595.1"
                     /translation="PGCGSCAGMFTANTMNCLTEVLGMALPGNGTIPAVDARRIRLAK
                     AAGMRIVDLIAKGIRPRNIMTEAAFTNAFTVDMAIGGSTNTVLHLPAIAHEVGLRLDL
                     DLINRISERTPHLAHLRPGGVHHIQDLDGAGGIPAVMAILGEAGLIDTSPITITGSTV
                     AANIATARVVRPEVIRTLKDPYHTEGGLAILRGNLAPDGAVVKQAAVAPEMMRHEGPA
                     RVFDGEEEALRAILNGAIHSGDIVVIRYEGPKGGPGMREMLGPTSAIAGMGLDKEVAL
                     LTDGRFSGASRGAAIGHISPEAMAGGVLALVKEGDRIRIDIPGRNLELLVAETELARR
                     RSEWQPPHPRVSGGYLSRYSMMVTSASTGAILGEAIGMRQ"
BASE COUNT         3073 a         3670 c         2888 g         2957 t
ORIGIN      
        1 agggctcctt ccagcgatac cgcatagtcc aggccccggc ccaggaagaa gatgtcctcg
       61 cagcccttga aatcctcggc gaactccttg atcttggtct cggtgttcaa caccttatcg
      121 atctgccccg gcaagttccc catggcttcc accatgccga ctcgctcatt ttgcgagagt
      181 gtgcctcggt gctgggccaa gaaaatggcc agtaaataca aggacaataa ctgggtggta
      241 taggccttgg tcgaagccac cgcgatctcc ggtccagcca aagtcaggat caaatcgtct
      301 gcctcccggg ccaccgagct gcccaccaca ttggtaatcg ccagcaccct ggagcccaaa
      361 cgtttggctt cccgcaaacc ggccaaagta tcggccgtct cgccggactg ggaaatgata
      421 atcgtcaacg atttttggct caccatcggg tccctgtagc ggaactccga tgccagatcc
      481 acctccaccg ggatgcgggc cagtttttcg atcaaatact tgccgaccat gccggcatgg
      541 taagcggtac cgcaggcgac aatttgaatc ttctccaggg tgtcgatctc ccggggatcc
      601 agtttgacct ccggaagcaa tattttcatt tcgacgtggt caaccttgcc ggccagtgtg
      661 tcgcggaccg ccttggcctg ttcgtggatc tccttgagca tgaagtcctc atagccgcct
      721 ttttcagcgg cggcaatgtc ccattgcaca tgatacactt ctttggaccg cggattgccg
      781 ttaaaatcgc agacctcagc cttttccgca gtgataaccg ccatttcccc gtcttccaga
      841 atataaatat cgcgggtata gggcaatacc gccggaatat cggaggctat gaaattctcc
      901 tctttcccga gcccgataat cagcggactg gccttgcggg ccaccacaat ctcgccggga
      961 tgctcgtcgc tgatcaccac cagcgcgaaa gcgccttcca cttttttgag cgcctcacgg
     1021 acagccgcag ccaggtctcc ttgataatac tcttcgatca ggtgcggcag cacctcggta
     1081 tcggtctcgg agttaaaaat atgccccttc tcctgcaacc attccttgag tccaatataa
     1141 ttttcgatga ttccattatg gaccagcgaa atcttttgat tgcaatcgaa atggggatgg
     1201 gcgttccgct ccgaaggccg tccgtgggtc gcccaacggg tatggcctat gcccaggttg
     1261 cctgtgagtt tgatttccgc caacctttcc tcaatcttac ttaaacgccc taccgttttg
     1321 caagaattca atttgccctg attatagaca gtaacccctg ccgagtcata accgcgatac
     1381 tccagccgct tcaaacccgc cagcaaaacc ggcaccgctt cttgtttccc aatatatcca
     1441 acaataccgc acatcggtaa aagtcctcct tataaataag attggattca tcatgattcg
     1501 tcgccaaatt ctttaccgtc tccgaattgg tgttccggaa attcatcgtt atttacctga
     1561 cactacgggt gggcaacgaa tggtcgactt taatgaggta agaaacctat atgtttatcc
     1621 cgaaaaatgc aacgccaaat aagggtcaaa ccgaaataaa aggtgaaccg ccaccttaaa
     1681 aatccggttt gaactagtct taggcgaatt cgggttaaca atactaaccc ttataaggca
     1741 acaggcaaac tacgaagcat ctactaacct cgtgcctcac gcctcatgcc gcgcgtccat
     1801 tggacgcgat ttgcccaagc taggacccta atcaaaacaa atctattcaa ttcacttgag
     1861 accctattgt tccaatcatc actgatgggt ttgtcaggcc tctaacggtg gtgtatcgcg
     1921 ttactttcgg taacgcgcct gcaccgaggg tcacccgccg attggtaacg ttcttttgaa
     1981 tgcttttttt gaatgttgtt tttgaatgtt gcattcaaaa cgttacgctc cgagatcccc
     2041 ttcctcgtca actctgaacc cggtttttac atcaaccggt ttggcaatgt cataagaccg
     2101 ttaaggttta agcaatcagt atttactcct gttgcctgtt gcctattgcc gcgcgtccat
     2161 tggacgcgat gcctcgcgct ctaggagcgc gctatccaga gccctggcgc taaatctttg
     2221 gtttgattct attcactgtt accggcatta ccaccccctt acattatatg cctcattgta
     2281 tcataaaggg ttcgaaaagg gaaccttcct taagttggtt ctttcttacg tgtatgtgaa
     2341 atcgagtttt aggttccttg ttccttttgg atgaccactg cgatagaccc gaccaactcg
     2401 cgcagtgtcg cctcttcagg cccttcagcc attacccgga tgagcggctc cgtccctgag
     2461 gcacgtacaa aaacccggcc cgaatctccc aacttttgtt tggcctggtc gatggcttcc
     2521 cgaatccggg gatttgcctc ccaaccttcc ttttgagcta cccggacgtt ttggagaacc
     2581 tgggggaaac gctccatctg acccgccagt tccgagagtt tccgtcccga tttgaccagt
     2641 accgcaatga gttgcaacgc agtcaaaatt ccatccccgg tggtattctt ctctaaaaag
     2701 atgatgtgcc ccgactgctc gccgcctaag accaagtcct gatcccgaag ggcttccaga
     2761 acgtaccggt cgccattggc ggtcaccacc acatccgcct gatgtttttt gaacgcctga
     2821 ctcaagccaa ggttggagta gacagtcacc gccaccttgt tatgccttaa cttgccttct
     2881 tgaagtaggg ccaacccaca gacggtgatg atctgatccc cgtccacgac ccggcccgct
     2941 tcatccacgg cgatcaccct gtcggcgtca ccgtcgtggg cgatcccaag attagctccg
     3001 taagcaacga cctcccgttg caagatctcg ggatgggtcg aaccgcattt gacattgatc
     3061 cggctgccgt cgtccatggc atgaagcgga atcacttcgg cccccaggcg ttgcatgatc
     3121 tgcggtgcca actgatatgc agcgccaaac ccgcagtcca ccaccacccg gagaccgtca
     3181 aaccggacct gcacggtgga gaccaggtaa tcctcatact gtttcaccgc gtcgtgggca
     3241 tcattgatct gaccgacctt gatcccggta ggccgttcga attgctcgag tccggtgcgg
     3301 agtatggctt cgatctcgtc ctccacttca tcggtgagtt taaaaccctg atggtcaaag
     3361 aatttgattc cattatcctc caccggatta tgggaagcgg agatcatcac tccggctaca
     3421 cttccgagcc tgcggcagag aaaggccacc ccgggagtgg gaagcacccc caaacggtaa
     3481 acatcgaccc ccaccgaagt gagcccggac atcagcgccg cttccagcat gtcgccggag
     3541 atccgggtat ccttgccgat gatgacccgc ggccgttcct ctgctgtccc ccgtccacgc
     3601 tgaaaataga tccctgccgc ttgccccagt ttaaaagcaa gttccggggt aagattctcg
     3661 ttggccactc cccggacccc atccgtgcca aataaacgcg ccatataaac ccctcctgat
     3721 tggatgctaa tattttaagt atgatttatt tacaatattt ctcgcagatt acttccgcag
     3781 tcttccaacc atccagtgcc gagctgatga tccccccggc atacccggcc ccttccccta
     3841 ccggataaat cccgttaaac ccttcggctt cccgttccac atcacggaca atccgcactg
     3901 gtgcagaagt tcgggtctct accccggtaa gtactgcatc cggccagtca aaccccttca
     3961 gctccctgcc aaactggcgg attgccgcgg ctattgcctg acaaagattc ggaggcaaca
     4021 cctcccatag attggcgggt cgggttcccg gaaggtacga cggtttaagt gcgataaagc
     4081 cctgagaagg ctcacatttt aaaaaatccg ccacccgttg ggcgggagca aaaaatttac
     4141 cgcccccggc ttcataagcc tttcgttcca agcgacgctg aaaatcaatc ccaccgagcg
     4201 ggcccgtatc cccataatcg gaggcaggaa ttgtcaccac aatcgcggcg ttagccacac
     4261 ccgaatttcg ggcatggtaa ctcatgccgt tggtcaccac cccgcccggt tcggaggcgg
     4321 ctccaaccac ctgtcctccc ggacacatac aaaatgtata tactccccgc cgactctccc
     4381 ggtcctgata agtcaggcga taatcccccg ggcccaattg cagattgcca gcccaactac
     4441 catattgcgc ccgatcaatc agatcctggg gatgctcaat ccgtaacccg actgcaatgg
     4501 gtttggcctc gatttttaga cctttttccg atagtaacgc atacaggtcg cgggcgctgt
     4561 taccaacggc cagtaaaacc agccccgtct ccaaatgcgt cccggaatta atcgatactc
     4621 ccctgatttc tccccgatct atccggagat cggtcaaacg gttacgaaat tgaatttcac
     4681 cgccgtattc cccgatcagc cgttcaagct tggtaactac ctcgcggagc agatcggttc
     4741 cgatatgggg taattgcagg taacgaatat cctcgggtgc ccccagttct atcaggactt
     4801 gaaggatttc ggccagccgc ggatctttga ttcgcgtagt aagttttccg tcggaaaagg
     4861 tcccggcccc gccgcaaccg tattgaatat tggactcggg atccaactcc ccggtatgcc
     4921 agaaatgatc tacccgggcg atcctttccc cgagtccctc cccacgctcg atgatcaacg
     4981 gcttaaagcc tgccgccgct agtttaaggc cagcaaaaag accggcaggg cccgcgccca
     5041 cgatcaccgg tctaaaagat attgacggtt tggtcttccc gacttggagt ggaacaaccg
     5101 ccggaggccc tgttacagta aggtcgagtt cggcatgttg tttcaaaaag gcatcctgtt
     5161 tttgaggcgc cagatcaatt cggagataga gattatagat aaaaaccggg cttttatgac
     5221 gggcatccaa cgatttcctg tcaatccgga cttccaaaac cgtccggggt tccaaaccca
     5281 accgccgggc tacccatgcg ggaagctgag ctacagttac gttaagcggc acgcgaagtt
     5341 gggctaattt cagccacatt cccataaaca acctttccta aacaattgtc attcatattt
     5401 ttaatagctc attgatccct tccccaccgc catatttgca ttccaagaat cctcgcgggg
     5461 gagggtcggg tgagggcctt atttaacctc agccttgcca ctaacagtcg tagccggttt
     5521 atcttgcatc ggttggatca gttcctttaa agtgcctttc aggcgctcac tgctgtaacc
     5581 ggtgtctaga accccattat acgccaggga gatccctccg gtctcctcgg agacgatcac
     5641 caccagggcg tcgctctggg atgccagact gaaacccgcc cggtgccgac tacctagttc
     5701 ctgggggagg ggcaattgat tatccagcgg cagaaaacat ccggccgccc gaatccggtt
     5761 ccccttgatc aaaatcgccc catcatgtag cggcgaaccg ggataaaaga tctcttcgat
     5821 caagatggcg gaaagggcgg cgtcgatata ggacccggac tcgctgacta ccgggagggg
     5881 atcctcccgc tccaggacga ttaatgcgcc aatcttttgt ttggccagac tctcgacagc
     5941 ctcggccacg atatcgatgc tctcgatggc gatgacaggt ggttttacga accatttgat
     6001 aatcggattt ttacgcccca gtatctccat ggcccgtttg agttcattct gaaagatgac
     6061 cggcagggcc acacccagca tgataatgat ccaggaaagc agaaggttta gatattcgag
     6121 tcccagcgcc ctgctgaaga tataggtcaa aaagagaatg atgatgccgt ttaaaaagaa
     6181 gactcccttg gtaccgcgca tcaatttaaa aagttgtgtg atgatcaaga taactagaaa
     6241 gagatcgatg gcggtccgga tgatattatg ctcgattaag aactgcagca aacgtctcac
     6301 caccattcat cagaaattcc aaggcttgcc caaccaactt cgttgattac cccaaccctg
     6361 ttgattaacc aaccctgttg gttattttac tatttgacat tagccctgaa aatcctttga
     6421 atcgatatga aaaaaaaccc gcttccaggg gaagcgggcc atcgatcatc gattacttcc
     6481 caatactggt cagaaagaaa tcaagcacga atgcggcaga aactagatac atcaaaggat
     6541 ggacttcctt atgcttgccg gaaactacct tgatcaaaac ataagaaatg aagccgtagc
     6601 caattccctt ggagatattg taggtaaacg ggatcattac catcgttaaa aatgccggaa
     6661 aagcagtgga aaaatcatcc caatgcatgt gcttgaccgc agttagcata agaaaaccta
     6721 cgataatcaa ggccggagcc accgcgtagg taggtacgat ccccaccagc ggcgtaaaga
     6781 agatggcaag caggaacaag aggccagtga ccacagaagt caacccggtc cggccaccct
     6841 caccgacacc ggaggcactc tccacataag ttgtgaccga gctacaaccg aacagaccac
     6901 cgacaaccgc agccagtgag tcgacgagca ataccttctt caaacgcggc agtttgccat
     6961 ccttacccac gaaccccgct tcttcaccga tggcaaatac cgttcccatc gtatcaaaga
     7021 agtcggtgat caaaaaggca aagatcgtgg cagccaagcc ccaactaagt gctcccttta
     7081 aatccagcgc aaagaaggtt tggaaatact cggggcggat cccggtaacc catgacccct
     7141 taggcaaatc caccaacccg gccaagaccg ccaggattgt tgaaaaagca attccaatca
     7201 ggatcgaacc cttgacctgt tttcccatga gtacggcaat gataaagatc cctgccaaag
     7261 cgatcaggct ttgggcggtc aatttaccaa aggtgatcaa ggtcaccgga tcggcaacta
     7321 ccaggttggc gtcctttaaa ccgatcagtg cgatgaataa cccgatcccc acaccaatag
     7381 cccgtttgag atcgagcgga atcgcatcca tcacccattc cctgaagttg gtcaaaacca
     7441 ggatggtgat gatcaatccc tcaatgacca caatacccat agccgtttgc cacggaacct
     7501 tcatccccaa aacaactccg taggccaaaa gggcattgag tcccattccg gaagccaggg
     7561 ccagcggata attggtgaac aaccccatgc agatggtggt aatacccgcc gccaagcagg
     7621 tggcaaagat caaggccggt accatgccct tccccatcgc atctcctaag atgctggggt
     7681 tgacaaagat gatgtaggcc atggtcataa aggtgacgca acccgccata acctccctac
     7741 cgaaagtggt tttgttctcc tgaaacttaa aaagtctttc gaacatcggt ctcctcctca
     7801 atataagtat ttgatgacgt ttttgtcgct agagtcaggg aaaaatgaat aaaccagcgt
     7861 gaggatccca tgattttaag atcctaaaca aaaaacccag cttagggcag aatcccccgc
     7921 tgggttattt aaacgaattg aaaaggccgc tcactggttg tccaattaac gggctccatc
     7981 tttccacaaa ctcctccatt ttggaacgcg acaaattatg gtgcaatctt accaaaaaaa
     8041 ttgggtcaag tcaataccac cgatttcacc ggaggttatt ctccacttgg gtgccggtag
     8101 acttgctctt tcaagacacc gatatacggc agattacgat atttctcagc gtaatcaagt
     8161 ccgtaaccga cgacgaagac atcgggaatg gtgaaaccgt tataatccgg ctgaatcgcg
     8221 accttcctcc ggtcgggctt gtccagcagt gtgcatagtt taattgagtt tagccgacgg
     8281 gccttcagat tttccaacag gtactgcagg gtcaaaccgg tatctacaat atcctctacg
     8341 atgatcacat cgcggccttc aattgccgca tccagatctt ttaagatccg gactgtaccg
     8401 ctggatttag tagaagcccc gtaactggag acagccatca aatcatacgc caacggcaga
     8461 tcgatctgcc gaattagatc cgccacaaaa ggaacagcac ctttgaggat ccccaccagt
     8521 agtagatcct tgcccttgta atcgttcgta atcatctggc ccaattcccg gacccgggcg
     8581 gatatctcct ccggccggta caatacttct tttagatcag cgtctagaat catgctctaa
     8641 cctcctgata taagtagata ttaaccgatc caccaggagg tgtcaacctt ttcttttatt
     8701 gaacagggca cctgcagctt gaatccggca aggttagata tttcccttct tgggtgtccg
     8761 gagcattgca atctggccgg tccggacgat ctcccggatc ccgaacggtt tcaatagggc
     8821 aatgatggcc tcaatctttt cactatcgcc ggtgatctcc acaatcacag cccggctgct
     8881 gacatcgata atctttgccc tgaagatatc cacgatctgc aggatctcgg aacggttctg
     8941 cgatccggca ttcaccttga tcaatgccag ttcccttccc accgtctcct tgaccggcaa
     9001 gtcgttcact ttgactacat cgatcagttt atagagctgt ttttcgatct gctccagaac
     9061 ctgttcatcc tcggcttcaa cttcaatgac catccgggag agttccggat cctccgatcc
     9121 ccccaccgcg atactctcaa tgttatagcc gcgccggctg aaaagtccgg ccacgcgtac
     9181 catgactccg gaacggttaa caaccaatgc cgaaataata tggcgcatca gagattcacc
     9241 ccaatcatcc ggtttagcgg agaattcgcc ggaaccatcg gcaatacgtt ttcctcccga
     9301 tccacccagc aatcgaccac caccggaccg ggttcactca aagccgcggc cagcgtttcc
     9361 ctgacctggt tcggctcggt gatccgataa cccttggccc cgtatgcctc ggcgatcttg
     9421 acaaaatcgg gattggagtc gagcacagtt ccggagtacc gtttattttg gaaaaactcc
     9481 tgccattgac gaaccatgcc caggtaaaag ttgttcagga tcactacttt gactgggatc
     9541 cggtgagcta cagccgttcc cagttcctga atggtcatct gaaagctgcc atccccggca
     9601 atgtcaatca ccattcggtc ggggttgcct acctgagcgc caatggctgc gggaaaccca
     9661 aatcccatgg tcccaagccc tccggaagta atcagcgaac ggggcttttg gaaccggaaa
     9721 aactgtgccg tccacatctg attctgcccg acctcggtgc agatgatggc ctcaccccga
     9781 gtcagctcac tcaatgtttc aatgacttgt tgcggtttaa atccggtcgg ttcgtaatgc
     9841 aacggatact ccttttgcca ttgaccgatc tgatcgcgcc actcaggatg ttttccagtc
     9901 cctgccttct cgtttagtgt ttctaagacg atcttcgcgt ctccaacgat ggggatatcc
     9961 acccgaacgt ttttaccaat ctccgccgga tcgatatcaa tatggatgat ccgcgcttgt
    10021 ggggcaaagc tgtccacttt acccgtgacc cggtcgtcaa accgcgctcc aatcgcgatc
    10081 aacaaatcag tggtaccgac tgcaaagtta gcggcagcag taccatgcat tcccagcatt
    10141 ccgagataca gaggatgacc ggagggaaaa gccgttaacc ccatcaaagt tgcggtcacc
    10201 ggaatatcca accgttccgc aaattggatt aactccgccg atgctcccgc cgcgatcacc
    10261 ccgccgccaa tatataaaag cgggcgtttc gcctccccga tcgcctccaa ggcttccttg
    10321 atctgccggg cgtttccgat ataattaggc ttataccccg gcagctcgac ggtttccggg
    10381 tattccaatt tcgctttggc ttgagtcaca tccttggcca gatcgatcaa tacaggcccg
    10441 ggtcggcctg tggccgcgat atgaaacgcc tccttcacta cccggggtat atcctcaatc
    10501 cgctttacca ggtaattatg cttggtaatg ggaatggtga tcccggtgat atccgcctcc
    10561 tgaaaggaat catagccgat taagaaagtc gggacctgcc ctgtgatagc caccagcggt
    10621 accgaatcca tataggcgtt ggcaattccg gtcaccagat tggtagcccc cggtccggag
    10681 gttgccaaac agactccggt cttgcccgta ctccgggcgt atccatctgc ggcatgaacc
    10741 gctccttgct catgccggac tagaatatgc cgtaaaggcg catcgtataa ggcgtcataa
    10801 atcggaatca cctgcccccc cgggtagccg aagatgacct cgaccccctc tcgcgccaaa
    10861 caatccagca aaatttcagc ccctgaattc ttcatttcgc accaacctcc agggtttttc
    10921 gactgacttc cgattccatc gatggataat aattgtaatt actataaatg ataaccagat
    10981 gaatgtcaag agaaaattag ataaaattaa gccacgtcaa atcagggttt gcggaacttt
    11041 tattaaaaaa agccttattt attgggtaga tcatttataa tagactaata tagtaagtaa
    11101 tgacagacat cacattaatt taaaatccgt tattattggt aaaaaacaaa ggaaccatcg
    11161 taggttcctc taacataaat cttatgagac aagcattagg ggattatcaa caaaaattat
    11221 ctctacactt aagggttttt acctggactg taaccaaaat atcctttgta acggagcatt
    11281 tttccaatat atccgagaaa tcgctgctgc ctgttgccta ttgcctgttc cctgttgtca
    11341 attaacaact cacccccgac ccctctcttt cagaaaagag aggggtgtag ggttatactg
    11401 aaagccaata gaaaacgtgg ggtgagttgc cgcgcgtccc gcaggacgcg atgcctcatg
    11461 cctattgcct catgcctatt gcttccccta aaatcgcccc ggtactggcc gatgtaacca
    11521 tcatactgta acgggagaga tatccgccgg aaacccgggg gtgcggaggc tgccactcgg
    11581 aacgacggcg cgccagttcg gtttctgcca ccaagagctc caaatttctc ccgggaatat
    11641 cgatccgaat ccggtcgccc tcttttacca gtgctaatac cccgccggcc atcgcctcgg
    11701 gtgaaatgtg accaatggcc gctccccggg aagcaccgga gaatcgcccg tcagtcaaca
    11761 aggcgacctc tttatccagt cccattccgg caatggccga tgtgggaccg agcatctccc
    11821 tcatgccggg tccgcctttg ggaccctcgt aacggatgac tacaatatct ccggaatgaa
    11881 ttgctccatt caagatcgcc cggagcgcct cctcttcacc gtcgaatacc cgggcgggtc
    11941 cttcatggcg catcatctcc ggagccaccg cagcctgctt aaccacggct ccatccggtg
    12001 ccagatttcc ccggaggatt gccaaccctc cctcggtatg gtaaggatcc tttagtgtac
    12061 ggataacctc gggccggacc acccttgctg tggcaatatt cgccgctact gtacttccgg
    12121 ttatggtgat tggggaggta tcgatcaatc ccgcttctcc aagtatggcc atgaccgccg
    12181 ggatcccgcc agctccatcc agatcctgaa tatgatgaac tccgcccggt cgcaaatgag
    12241 ccagatgcgg ggtacgttcg ctgatgcgat tgattaaatc cagatccagt cttaatccaa
    12301 cctcatgagc aatggccgga agatgaagca cggtattggt cgatccgccg atagccatat
    12361 ccacggtaaa cgcattggta aaagccgcct ccgtcataat attccgcggc cgtattccct
    12421 tggcaatcag atcgacgatc cgcatccctg cggctttggc caaccggatc cgccgtgcat
    12481 caaccgccgg aatggtgcca ttccccggaa gggccattcc caaaacctct gtcaggcaat
    12541 tcatcgtgtt ggcggtaaac attccggcgc aggaaccgca gcccgggc
//
LOCUS       DASVMB010000034         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_371690,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000034 DASVMB010000000
VERSION     DASVMB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371690"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VF531_01130"
     CDS             <1..1215
                     /locus_tag="VF531_01130"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7712596.1"
                     /translation="NQRIGRKLLITGSGRCNLTNQSNLEEIIGNIPGNGRFLYSALRR
                     FGPEELMALFEDEFGVALKVERGHRVFPESDQAQDIVRALQNALKDYGVTLWCGARVL
                     DIDLSGAPQVFRVTGEKGLHLDCRYVTVATGGASYPATGSSGDGYRLARSAGHQIIPA
                     QPALVPLETKENWPKQLQGLSLTNVAVTAWYRDKKLQAEFGEMLFTHFGVSGPIILSL
                     SREIVQRLANDPGSVKLILDLKPALNETQLDLRLQRDFTKFVRKIYQNALDELLPKKM
                     IPIIVEQSGIDPSKPVHQITKEERLRLVHLLKNLELTVTQPRPLSEAIVTAGGVSTKE
                     IDPKTMESKIVPGLYFVGEVVDVDAYTGGFNLQIAFSMGYAAGREIAARAAASKGFSE
                     GDAIVSQKVPLV"
     gene            1504..1923
                     /locus_tag="VF531_01135"
     CDS             1504..1923
                     /locus_tag="VF531_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HEX7712597.1"
                     /translation="METGSKEVAKAALAMAMTSSREEERLLKEDLKNHEIRAAAVDYG
                     GEAIMAVKTILERTVVASKRELLIKECHAEEGAIAGATREAMSQIISKAVGLSIGGKV
                     GLARKGDHVCVAVFFSIGLIHLDEVAVGLSHRAIGGG"
     gene            1924..2310
                     /gene="aroH"
                     /locus_tag="VF531_01140"
     CDS             1924..2310
                     /gene="aroH"
                     /locus_tag="VF531_01140"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX7712598.1"
                     /translation="MKLWAVRGGITISQDSAAQVVQATRELLEKLLANNRIDPEDIVS
                     ILFTVTPDITSEFPAVAAREIGLVEVPLMCAQEIPKPGALPLCIRVMLHFYTSLKRNQ
                     IQPVYLRDAVKLRPDLAARQTTSTRV"
     gene            2361..>3067
                     /locus_tag="VF531_01145"
     CDS             2361..>3067
                     /locus_tag="VF531_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7712599.1"
                     /translation="MNATKLARVCVQKLPAYIPGKPIEEVEREFGVSGVIKMASNENP
                     LGPSNEVRQAVIQALERLAYYPDANAYYLKEALAGELAMDPSNFLIGNGLDDVNRIMA
                     ETFLKPTDEVVIPKPTFSMYQTVTLLMGAKPVIIQGGHGLGNDLEAMKNAITAKTKLI
                     FICNPNNPTGTIVKRNELVGFLKTVPSKTLVVLDEAYSDFADDPEFPNGIELLKEGHT
                     NLIVFRTFSKIHAMAGLR"
BASE COUNT          806 a          666 c          786 g          809 t
ORIGIN      
        1 aatcaaagga ttggaaggaa gcttttgatc accggttcag gacggtgtaa tcttacaaat
       61 caatcgaatc ttgaggagat tatcggcaat attcctggaa acggccgctt cttatacagc
      121 gctttgcgtc gcttcggacc agaggaattg atggctttgt ttgaagatga attcggagtg
      181 gccttgaaag tggaacgggg ccatagggtt tttccagaat cggatcaggc tcaggatatc
      241 gtcagggcat tacaaaacgc tctaaaagac tatggtgtga ctttatggtg tggcgcacga
      301 gttctggata ttgatctctc gggtgcgcct caagttttta gagtgaccgg cgaaaaggga
      361 ctccatttgg attgccgcta tgttacagtg gcgaccggag gggcttctta ccctgctacc
      421 ggcagcagcg gtgacggtta ccgcctggcg cgatcggcgg gacatcagat tattcccgct
      481 caaccggctt tggtgccgtt ggagaccaaa gagaattggc ctaaacagct tcaaggcctt
      541 tccttaacca atgttgccgt caccgcctgg taccgggata agaaactcca ggcggaattc
      601 ggtgaaatgc tctttaccca tttcggggta tccggtccga ttatactcag tcttagccgg
      661 gagattgtcc aaaggctcgc aaatgatccg ggttcggtca agctcattct cgatttgaag
      721 cccgctttga atgaaactca attagatctt cgattacagc gagactttac aaaatttgtt
      781 cgaaagattt atcaaaacgc attggacgag ctgttaccga aaaaaatgat ccccatcatc
      841 gtggagcagt ccggaattga tccatcgaaa ccggtacacc agatcacgaa ggaggaaagg
      901 ctgcgattag tccacctcct aaagaatctg gagctcactg taactcaacc ccgtccttta
      961 tcggaggcga ttgtaaccgc cggcggcgtc agcaccaagg aaatagatcc caaaaccatg
     1021 gagtcaaaaa tcgttcccgg actttatttt gtaggtgaag tggtggatgt agacgcttat
     1081 accggggggt ttaatctcca aatcgcattt tccatgggtt atgccgcagg aagggagatt
     1141 gccgcaaggg cggccgcatc aaaagggttt tcggagggag atgccattgt ttcacagaaa
     1201 gtaccgctgg tatgaacggc tttttttaag acttaagttc catgaaatag gatgtatgaa
     1261 acttattttc ggattaaccg ttttgttaat cataacccaa attcttctga ctttcggttg
     1321 gttcagaaat ggttgtgttt tatcggaaag attggaaggt tcgcctgtaa aattttataa
     1381 aagtttaaat cattgatgtc ttgaaccaag aaccgaatta tgataaattt actttaatat
     1441 gctagcatta tgtccggcac cgccagggtg cttttttcat ctgaatgtag gaggcactga
     1501 cgtatggaga ccggaagcaa agaggttgcc aaggctgctt tggcgatggc catgaccagt
     1561 tcgcgtgagg aagaacggtt attgaaagaa gatttaaaaa atcatgaaat acgcgccgct
     1621 gctgtggatt atggtggtga ggcgattatg gccgtaaaaa ccattctgga gcgaaccgta
     1681 gttgcctcca aacgagagtt gctgattaaa gaatgtcacg ccgaggaagg agcgatcgcc
     1741 ggtgctaccc gtgaggcgat gtcccagatc atatccaagg cggtgggatt aagtatcggc
     1801 ggtaaggtcg gtttagcccg taaaggcgat catgtgtgtg tggctgtttt ttttagcatt
     1861 ggcctgattc atcttgatga agtcgcggtg gggttaagcc accgcgcaat tggaggcggt
     1921 tgaatgaaac tctgggctgt tcgaggtggg attaccattt cccaagactc tgccgctcaa
     1981 gtggtacagg caacccgtga attacttgag aagttgctcg caaataaccg gatcgatccc
     2041 gaggatattg taagtattct ttttacggtc acccccgata tcaccagtga atttccggct
     2101 gttgccgcca gggaaattgg tttggtcgaa gtaccgttga tgtgcgccca ggaaattccc
     2161 aaaccgggag ccttaccttt atgtatccgg gtgatgctgc acttttacac ctctttgaaa
     2221 cggaaccaga ttcaaccggt atatttacgc gatgcagtca aattacgtcc ggatctagcc
     2281 gcgcgtcaaa ccacctccac tcgagtatga catctcatta ttatcaaatc tcgattttct
     2341 gactttaaag gagaacttct atgaatgcaa cgaaattggc acgtgtatgc gtgcaaaaac
     2401 tgcctgctta tattccgggg aaacccatcg aagaagtaga acgcgaattc ggggtcagcg
     2461 gtgtgatcaa gatggcttcc aacgaaaatc cgcttggtcc gtccaatgaa gttcgtcaag
     2521 ctgtaatcca ggccttggaa cgactggcat attatcccga cgccaatgcc tattatttaa
     2581 aggaagcctt agccggcgag ctggctatgg atccatccaa ctttctaatt ggaaacggat
     2641 tggatgatgt caatcggatc atggccgaaa cctttttgaa gcccaccgat gaggtggtta
     2701 tacccaaacc gactttcagt atgtatcaga ccgttacttt gctgatgggc gccaaaccgg
     2761 ttataattca aggcgggcat ggcctaggaa acgatctgga ggccatgaag aatgcgatca
     2821 ccgctaagac caagttgatc ttcatttgta atccgaataa tcctacaggg accattgtaa
     2881 agcgtaatga attggtcggt tttctaaaaa cagttccatc taaaacactg gtggtgctgg
     2941 atgaagctta ctcggatttt gccgatgatc cggagtttcc aaatggaatc gagttgttga
     3001 aagaaggcca taccaacctc attgtttttc ggactttctc caagattcat gccatggccg
     3061 gattgcg
//
LOCUS       DASVMB010000035         5328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_445833,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000035 DASVMB010000000
VERSION     DASVMB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445833"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF531_01150"
     CDS             <1..206
                     /locus_tag="VF531_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX7712600.1"
                     /translation="VGWDDIIQRQILLGGKGVIRNFSRGGRHTIATIGFLQHFYLYCQ
                     ANRDNFRTSWTQWLQQNPLPGAK"
     gene            complement(243..812)
                     /locus_tag="VF531_01155"
     CDS             complement(243..812)
                     /locus_tag="VF531_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX7712601.1"
                     /translation="MFLYLKLFYVFFKVGLFSFGGGYAMLPLIYQEVQTLNLMPAKEF
                     SNVVGLSQVTPGPIAVNAATYVGFQSAGFWGALIATLGVSLPCFILILMIEAFMNRFQ
                     TNSVVQGVLAGIRPATVGLIFSAAVFFGQTSLFGPKLFSSELFIHPLSQIHLPALAIF
                     VMTIIANRKWHLDPILLTVLAGIAGALIL"
     gene            complement(812..1372)
                     /locus_tag="VF531_01160"
     CDS             complement(812..1372)
                     /locus_tag="VF531_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX7712602.1"
                     /translation="MQKQSEPQRLWELFWAFLKIGSFTFGGGLAMLPLIHKEVVDNRK
                     WLADKEMVDILAISQSVPGVIAINSAIFVGHRVKGVSGAVAAAIGVTLPAFLSIILIL
                     LVLTNLRQNPYVDKAFAGIRAASAALILLSGIKLGQSILKERSGYGIAIVSFIAIAVL
                     NISAVWAIIFGGMAGFILYRNGERSH"
     gene            complement(1770..4019)
                     /locus_tag="VF531_01165"
     CDS             complement(1770..4019)
                     /locus_tag="VF531_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7712603.1"
                     /translation="MRKVTAINNNWYYQKSYQDGMEKRRDLEGFEKVNLPHANIELPY
                     NYFDERLYQIKSCYKYPISIPREERGKRIMLHFEGVMAYAKVYLNGVFLGEHKGGYTP
                     FDIRIDPAFDYESNQNTLTVVVDSSELPDIPPFGGQIDYLTYGGIYREVSLGSYDKIS
                     IHNIKVDTDGMFEAEKSALVKVYLDNPEQVCGNGEFRIEITDRSGAKIGSECFHRDID
                     GSTSGHEFKLNGLKDVKLWDIDHPHLYQVKVEFKLNEYSDAYADSFGFRKAEFRPDGF
                     YLNGTRLKLRGLDRHQAYPYVGYAMPKRVQAKDAAILKNELHLNIVRTSHYPQSKHFL
                     NQCDVLGLLVLEEIPGWQHIGGPEWKERAKENVREMVERDWNHPAIVLWGVRINESRD
                     DDEFYTATNRIAHELDATRQTGGIRCITHSHLLEDVYTFNDFIHNGIRKPLRYQKEVT
                     GLEYPVPYLVTEYNGHMYPTKRFDQEERQIEHSLRHLRVQNAAGLDDSISGAIGWCAF
                     DYNTHKDFGSGDRICYHGVMDMFRLPKFASYVYRSQVSPEVEPVLEPVTFWARGERSI
                     GGVIPLVIFTNCDYIEFQYGNSQKIYKAYPRKEEYPGMPYPPVMIDDTVISPEETGAW
                     GMAWEDALIRGYYQGRLIIEKRLCKDPLPGELFVAADDPALDASEKDATRVVVKVLDQ
                     YGNLLPFCDEIIQLEVKGPGRLIGPRQVAVKGGAVAFWVETTNQAGWIEVIVQSQSLG
                     RKDLRIEVK"
     gene            complement(4007..>5328)
                     /locus_tag="VF531_01170"
     CDS             complement(4007..>5328)
                     /locus_tag="VF531_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX7712604.1"
                     /translation="QELESGITDPKLKKMAMASLLNTWETTTFLLEDKTTYIITGDIK
                     AMWLRDSSCQVEPYLHFADTDPYIKEVLLGLAQKQAFYLCIDPYANAFNQEATGVEQF
                     PEDRTAKNPWVFERKFELDSICHPVRFWWTLWRRTGDRGLFTPMIHEALTQVVKVMEI
                     EQHHKQSEYSFERQEGPLSDTLACHGLGTPVADTGMIWSGFRPSDDACMYHYLIPAQL
                     FAAQTLLYIATIAGFIYDDQPLLHKAQQLRSAILSGVESFGKINHPEFGTIYAYEADG
                     LGGINLMDDANVPSLLSLPYLGVCYPNDPVYLNTRRFILSRNNPYYSIGRCAKGIGSP
                     HTPDRYIWPISLIIQGMTSVNPAEADELLRMVMATDGGTGLMHEGFNPDDPKQFTRAW
                     FAWANALFGEWILRRLGFEPYNPLHDPELLEVSKIHQTITGGNQCEK"
BASE COUNT         1365 a         1460 c         1215 g         1288 t
ORIGIN      
        1 gggtcggctg ggatgacatt attcaacgcc agattttact tggtggtaaa ggagttatcc
       61 gtaacttcag ccgcggcggc cgacatacca tcgccactat cgggtttttg cagcactttt
      121 acctttactg ccaagcgaac cgggataatt tccgtacatc ctggacccaa tggctgcagc
      181 agaaccccct gccaggagcc aagtgacacc ggatcaggtc aacccaattt ttagccgggg
      241 ttttaaagaa tcaaggctcc ggcgatccct gccagaaccg tcaggagaat cgggtcaagg
      301 tgccactttc tattggcgat gatagtcata acgaagatgg ccagggccgg tagatggatc
      361 tggctcaatg ggtggatgaa cagttcggaa ctgaatagtt tcgggccgaa aagcgaggtt
      421 tgaccaaaaa agaccgcggc ggagaagatg agccccactg tagccggccg gatccctgcc
      481 agtaccccct ggactaccga gttggtctga aagcgattca taaaagcctc aatcatcaga
      541 atcaggatga agcaaggcag agatactcct aaagtcgcaa tcaacgcccc ccaaaaaccg
      601 gccgattgaa accccacata cgtagcagca ttgactgcaa ttggacctgg agttacctgg
      661 gacaacccca ctacattgga gaactccttg gccggcatca ggttcagcgt ttgcacctcc
      721 tgataaatca ggggcaacat ggcgtaaccg ccgccgaaac tgaacaatcc cactttgaag
      781 aagacgtaaa aaagcttgag gtataaaaac atcagtggct cctttcgccg ttacggtaga
      841 ggatgaagcc ggccattccg ccaaagatga tggcccagac ggcactgatg ttgagaacgg
      901 cgatggctat aaaggagacg atggcaatcc catagccact tcgctccttc aggatggact
      961 gacccagttt gattcctgac aacaagatca gtgcggccga tgctgcccgt attcccgcaa
     1021 aggccttgtc cacataaggg ttctgcctca ggttggtcag aaccaacagg atcaggatga
     1081 tcgaaagaaa ggccggtagt gtaactccga tggccgccgc taccgcaccg ctgaccccct
     1141 tgacccgatg gccgacgaag attgcggaat tgatggcgat aacacccggc accgattggg
     1201 aaattgccaa gatatccacc atttccttgt cggccaacca tttcctgtta tccaccactt
     1261 ccttatggat caagggcagc atggccaaac ccccgccaaa ggtgaaagag ccgattttca
     1321 gaaaggccca gaacaactcc catagtcttt ggggttcaga ttgtttttgc atgaaggttc
     1381 tccgcttatt catgattggt tactcaagcc acatcggatc ctcgaaatta cacgaaacag
     1441 tttatgatct cccgattttt tcgtgtcatc cgtaaccagc tccttgtacc ctattatacg
     1501 cgtatactcc cgtcctacgc tagtatttcc tttaccggaa caatcccgca agcgtcgcct
     1561 tcagacccgc tccaaaagac gttggttcaa acacatgtac cctctggata cacagccgcc
     1621 atcaaaaaaa tggctgaacc tcttgcccgc cgtccggtgc attttaaggt tcagccgtaa
     1681 attgtgaatc cagaaaccgg catccggcaa ccgaatgaat ctgttggccc gaaaacatcc
     1741 aagtcccggt atttatcgga aaccggattc tatttaactt caatcctcag gtccttccgt
     1801 cccagggatt gcgattggac tataacctct atccaaccgg cttgattggt cgtttcaacc
     1861 cagaacgcca ctgccccgcc tttgaccgcc acttggcgcg gcccgatcaa ccgccctgga
     1921 ccctttactt caagttggat gatctcatcg caaaaaggca acagattacc atactgatcc
     1981 aatactttta ccactacgcg ggtggcatcc ttctcggacg catccaacgc cggatcatct
     2041 gcggccacaa acaactcgcc gggcagcggg tccttgcaaa gtcttttttc gatgataagc
     2101 cggccttgat aataacccct gatgagcgca tcctcccatg ccattcccca cgccccggtt
     2161 tcttcaggac tgatgacggt atcgtcaatc atcactggcg gatagggcat gcccgggtac
     2221 tcttccttac ggggataggc cttgtaaatc ttctgcgagt tgccgtattg aaactcaatg
     2281 tagtcgcagt tggtaaagat aaccagggga atgacccctc cgatgcttcg ctcgccccgg
     2341 gcccaaaagg ttaccggctc taaaaccggc tcaacctcgg gtgaaacttg agacctatat
     2401 acataagagg cgaatttcgg caaacggaac atatccatga ccccgtgata acaaatcctg
     2461 tcgccggagc cgaaatcttt gtgggtatta tagtcaaagg cgcaccaacc gatggccccc
     2521 gaaatactgt catccaaacc ggccgcgttt tgaaccctta aatggcgcaa cgaatgctct
     2581 atctgtcttt cttcctggtc aaaccgcttg gtgggataca tatggccgtt atactctgtg
     2641 accaggtaag gaaccggata ttccaacccg gtaacttctt tctgataccg caagggctta
     2701 cggataccat tgtgaatgaa gtcgttgaaa gtatagacat cctccagcaa atggctgtgg
     2761 gtgatgcaac ggatgccccc cgtctgccgg gttgcatcca attcatgggc aatccggttg
     2821 gttgccgtat aaaactcatc gtcatcccgg gactcgttga ttctcactcc ccacaacaca
     2881 atcgccggat ggttccaatc ccgttcaacc atctcccgga cattctcttt ggccctctcc
     2941 ttccactccg gaccgccaat atgttgccat cccggaatct cttccaggac caacagtccc
     3001 agtacgtcgc attgattcag aaaatgcttg gattggggat aatgggaggt acgaacgata
     3061 tttaaatgca attcgttttt gaggatagcg gcatccttcg cttgaacccg tttgggcatg
     3121 gcgtagccca cataaggata tgcttgatgc cgatccaacc cccgcaattt tagcctggtt
     3181 ccgttcagat agaacccatc cggtctaaac tccgccttcc gaaacccgaa actatccgca
     3241 tacgcatcgg aatactcgtt caacttgaac tccactttca cctggtacaa atggggatgg
     3301 tcaatatccc acaactttac atctttcaaa ccgttcagtt tgaattcatg acctgaagtc
     3361 gatccgtcaa tatcccgatg aaaacattcc gaaccgatct ttgcgcctga cctgtcggta
     3421 atctcgatcc ggaactcccc attgccgcag acctgttccg gattgtcaag atacacctta
     3481 accagggcgc ttttctcagc ctcaaacatc ccgtccgtat ctaccttaat gttgtgaatc
     3541 gatatcttat cgtagctacc caggctcact tcgcgatata tcccgccgta ggtcaggtag
     3601 tcgatttgac cgccaaaggg cggtatatcc ggcaattcgg aagagtcaac cacaactgtc
     3661 agcgtattct gattcgattc atagtcaaag gccgggtcaa tccggatatc aaacggcgtg
     3721 taaccccctt tgtgctctcc cagaaataca ccgtttaggt agaccttggc gtaggccata
     3781 accccttcaa aatgcagcat gattcgcttg ccccgctctt cccgaggaat ggagatggga
     3841 tacttataac aggatttaat ctgataaagc cgctcatcga agtaattgta cggcagttcg
     3901 atgttggcgt ggggcaggtt taccttttca aatccctcca aatcccggcg cttttccata
     3961 ccgtcttgat aagatttttg atagtaccag ttgttgttga tggcagtcac ttttcgcatt
     4021 ggtttcctcc ggttattgtt tgatgtatct tggatacctc cagcagttcc ggatcgtgta
     4081 atggattata tggttcaaaa cccagtcgcc tcaaaatcca ttccccgaat aaagcgttag
     4141 cccatgcaaa ccaagcccgg gtgaactgct tcggatcatc cgggttaaaa ccctcatgca
     4201 tcaacccggt tccgccatcg gtggccataa ccatccgtaa caactcatcg gcttcggccg
     4261 ggttaaccga ggtcattccc tgtatgatta gggagatagg ccaaatataa cgatccggtg
     4321 tatgagggga gccgatcccc ttggcgcacc ggcctattga ataataggga ttattccggc
     4381 tgagaataaa tctcctggta ttcaaataaa ccggatcatt agggtagcat actcccaaat
     4441 aaggcaagga aagtaaactg ggaacattgg catcatccat cagattgatt cccccaagac
     4501 catccgcctc atatgcataa atcgtcccga attccggatg attgatcttt ccgaaggatt
     4561 ccactccgga gagtattgcg gatcgtaact gctgcgcctt atgtaacaag ggttgatcat
     4621 cgtatatgaa tccggcaatc gtcgcaatat ataataatgt ctgcgccgca aacagttggg
     4681 ccggaattaa ataatgatac atacatgcat catcgctggg ccggaacccg gaccagatca
     4741 tcccggtatc ggctaccgga gtccccaagc catgacaggc caaagtgtcg gaaagcggac
     4801 cctcctgccg ttcaaacgaa tactccgatt gcttatggtg ttgctcaatc tccatcacct
     4861 taaccacttg agtcaatgcc tcatgtatca ttggagtaaa tagtccccga tccccggtac
     4921 gccgccataa ggtccaccag aaccgaacgg gatggcaaat ggagtccaac tcaaatttcc
     4981 gctcaaagac ccaggggttt ttggcggtgc gatcctcagg aaactgctcc actccggtgg
     5041 cttcctgatt aaaggcattg gcataggggt caatgcaaag ataaaaagcc tgtttttgag
     5101 ccagacccaa taaaacttct ttgatgtacg gatcggtatc ggcaaaatga aggtagggct
     5161 caacctggca agaactatcc cgtaaccaca tggccttgat gtcaccggtg atgatatagg
     5221 tcgttttatc ttccaataga aaagttgtag tttcccaggt atttaagagg gaggccattg
     5281 ccattttctt aagcttggga tccgtgatcc cggactccaa ttcctgaa
//
LOCUS       DASVMB010000036         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_3602,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000036 DASVMB010000000
VERSION     DASVMB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3602"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VF531_01175"
     CDS             <1..688
                     /locus_tag="VF531_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEX7712605.1"
                     /translation="RMDGTFRTETGSCASCGNCAEVCPTRARYRIGSKYTVRELFSEV
                     QRDAVFFAESGGGITLSGGEATIQSQFVAAFLRLCQNTGIKTAIETNGFFDRERFTKL
                     LPFLDQIFFDIKMISPAEHQQYTGAHNDIILDNAGWLARRFTKLTFRTPLVQGMTLTD
                     RNMKDIAAFLIGLKVKTIELCLYQNSWEKKLQWLNTRQEPLNLAPLLPWDVAGIIEFF
                     ANSGIEAKII"
     gene            771..1205
                     /locus_tag="VF531_01180"
     CDS             771..1205
                     /locus_tag="VF531_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7712606.1"
                     /translation="MDNSNLLRDLVRALEKNIGAFEKSKMKCCGLNIGQCYVVLEIGL
                     ADEISLIDLAKLLNLDKSTMSRAVNNLVNAGLVDRLIDAGNRRYVKLQLTEQGQSAFT
                     IINATFDSYLMSILDAIPEDKRDQVMESLKILIVALNKNQCC"
     gene            1504..3552
                     /locus_tag="VF531_01185"
     CDS             1504..3552
                     /locus_tag="VF531_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7712607.1"
                     /translation="MNNPDERSTHPVSEPNTVEARPTFPKRAIITAGMPYGNKELHLG
                     HIGGVFIPADMYARFLRDRIGRENVIFVSGTDCYGSPILEYYRQLVEKGSFDGTIDQF
                     VESNHNKQEEILKAYDIDINLFAASSFSRSAEIHRQVSDELINRMYANGYLVKMTTSQ
                     FYDAELGVFLNGRQVVGQCPIMGCQSERGYADECSLGHQYMPIELINPQSTLTGKKPE
                     MRDVTNWYFKLDKFHDLLEKWVDVFGKRPNCRNFVVKSIQEFLEPPVIYVKRDQLDSL
                     NAMKEKLPEFTLEDKENKSSVLLIFDELENREKACLILAEYGIRFRTGKTLVPFRLTG
                     NIEWGVPAPALEGLDGLTIWVWPESLWAPLSFTKTYLEKQGEDNTAWKDWWCSKDSKV
                     YQFIGEDNVYFYGPVEMAMFMGTQGKAPSTNPPEGELQLPDLIVNNHVLFLNKKASSS
                     GAIKAPMAKDLLDYYTAEQLRAHFLGLGLGIRSVSFQPKPLNPMASEKDSDPVLKEGN
                     LLTNVFNRAVRSCFYTAQKYYDGRIPVGEIGEEILKESEKTILEYERLMYKCDFHIVM
                     NLMDTFIRNINKYWSKNMREAEENNNGTLRLQTLVDTFHMVRTAAVLMHPIAPQGTEM
                     ILDYLNLGEEFWNWDRIFEPLYAFMKDPQNHKLKFLEPRVDFFKKHPSQIAQMNESGD
                     "
     gene            3778..4890
                     /locus_tag="VF531_01190"
     CDS             3778..4890
                     /locus_tag="VF531_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX7712608.1"
                     /translation="MNGDWAYLSREPLVLRSNRVWRTYQGGMKIEEWQGKPDPKDGDY
                     PEEWVASTVKARNPGREAYGDEGLSKLKIDGETTTLKQLIDSDPGAFLGEKHVQKYGS
                     NMALLFKVLDSKCRLSIQVHPDRKFSNQVFNSPFGKTEAWFVLGSREISQTESYLLFG
                     FKPGVTRERWRELFERQDIEGMIDCLHKIPVIPGEVWLIEGGLPHAIGPGCFLIEVQE
                     PTDYTIRVERTTIDGRKLPDVLCHQGAGFDKIFDCFHYDESCSYEETFKKWRITPRPV
                     QSVPGAEVMELVGKADTDKFRLYKYEVSGSLNCRDERVYSIVTVIAGSGRILYQNGQT
                     FIGRGDQLFLPANLGDFKWQADPGSKLVLARAYPPD"
     gene            4960..5292
                     /locus_tag="VF531_01195"
     CDS             4960..5292
                     /locus_tag="VF531_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7712609.1"
                     /translation="MSCYIRVKDAEVEQQGPSKTYLLLSDKQGSVKGCCSGLNLLLNE
                     EYNTPGVHEDQEGFFVIEGTGWAKFGDEEFRLEPDMAMLAPAGVAHCMKKDPGAKVLK
                     VLWFHAAI"
BASE COUNT         1488 a         1185 c         1455 g         1369 t
ORIGIN      
        1 ccggatggat ggcaccttcc gtactgagac cggcagctgt gcatcctgcg ggaattgcgc
       61 ggaagtatgc ccaaccagag cccggtatag gatcgggagc aagtatacgg tccgggaatt
      121 gttttccgag gtacagcgtg atgcggtatt ctttgcggag tcgggcggcg ggatcacctt
      181 gtcgggaggc gaggcgacga tacaatctca gttcgtggcc gcgtttttga ggctctgcca
      241 aaataccggt atcaagaccg ccattgaaac caacggcttt tttgaccggg aaaggttcac
      301 gaagttattg ccctttctag atcagatttt ttttgatatc aaaatgattt caccagcgga
      361 acatcaacaa tacaccgggg cgcacaatga catcatattg gataatgccg ggtggctggc
      421 acggcgtttt accaagctta cgtttagaac gcccctggtg cagggtatga cactgactga
      481 ccggaatatg aaagacattg cagcattcct tatcggatta aaggtcaaaa cgatagagct
      541 ctgcctgtat cagaacagtt gggaaaaaaa gttgcagtgg ctgaatacca ggcaggaacc
      601 attgaacctg gcaccgcttt tgccgtggga cgttgccggc atcatcgagt tctttgcgaa
      661 ctcgggtata gaggcaaaaa ttatctgaac atcgatgctt aatcgcaaat gaataatagg
      721 gttgcaggat ccaacagttg tatgttaccc tgtattgggg tgatgccgga atggacaatt
      781 caaatttatt aagggatttg gtaagggctt tggagaaaaa tatcggggca tttgaaaaga
      841 gtaagatgaa atgctgcggc ttaaatattg gacaatgcta cgtggttttg gaaatcggac
      901 ttgccgatga gatttcttta attgatcttg ccaagctgct gaaccttgac aaaagcacga
      961 tgagcagggc tgtgaataat cttgtcaacg ccggactggt agaccggctc attgatgccg
     1021 gcaacaggag atatgtcaag ctccaattga ctgaacaggg gcaaagtgcc tttacgataa
     1081 tcaatgcaac gtttgattcg tacttgatga gcatcttgga tgctataccg gaagataaaa
     1141 gggatcaagt gatggaaagc cttaaaatat taatcgtggc gttgaacaaa aaccaatgtt
     1201 gttgatgagt taagctattt gcggatattg tgaaatatac gtatgaataa cagggggtaa
     1261 caatcttcgt acccgggata ttcttcacaa aagcattgcc gttcgcggca ggatttacat
     1321 aactttcatc gaaacaatca acaatctttc attcgtattg ctgaaatatc tgggataatt
     1381 caaacttcat ttgtcgtcag acttcgtata gaaattgcgt attttgtata ccatggtaaa
     1441 agcattgtcc gggtaaacat ataatgatga actgagataa aacaccgaaa ggcaggaaac
     1501 ctcatgaata acccagatga aagaagtacc catcccgtat cagaacccaa taccgttgaa
     1561 gcaagaccca cgttccccaa acgcgcgatt atcaccgcgg gaatgcctta tggcaataaa
     1621 gagcttcatc ttgggcatat cgggggcgtt tttattcctg cggatatgta tgcccgtttt
     1681 ttacgggacc gcattgggag agagaacgtc atctttgtat cggggacgga ctgttacggt
     1741 tctccgatat tggaatatta ccggcaattg gtggagaagg gttcatttga cggcactatc
     1801 gatcagtttg tagagtcaaa tcacaacaaa caagaagaga tactcaaggc gtacgacatc
     1861 gacatcaatc tcttcgccgc ctccagtttc agccgctccg ccgagattca tcgccaagtt
     1921 tccgatgaat tgataaacag gatgtatgcc aacggttatc tggtcaaaat gaccacctcc
     1981 cagttttacg atgccgaact cggggttttt ctgaatggac gtcaagtggt ggggcaatgc
     2041 ccgattatgg gttgtcaatc ggaacggggc tatgcggacg aatgttccct gggccatcaa
     2101 tatatgccga tcgagctgat caatccccag agcactctca cggggaaaaa accggagatg
     2161 cgggatgtca cgaactggta ttttaagctg gacaaatttc atgatctgct ggagaagtgg
     2221 gtggatgttt tcgggaaacg tcccaattgc cgcaattttg tcgtgaaaag cattcaagag
     2281 tttctggagc cgccggtcat ctacgtcaag cgggaccagc tggattcatt gaacgcaatg
     2341 aaagaaaagc ttccggaatt tacccttgag gacaaggaga ataagtcatc cgttctctta
     2401 atctttgacg aactggaaaa tagggaaaaa gcatgcctga tcctggcgga atacggtatc
     2461 cgttttcgga ccggcaaaac gttggtgcct ttccggttaa ccgggaatat cgaatgggga
     2521 gttccggctc cggccctcga agggctggac ggtttgacga tctgggtatg gccggagtct
     2581 ctctgggcgc ccctctcctt taccaagact tatcttgaga aacaaggcga ggacaatacc
     2641 gcctggaagg attggtggtg ctccaaggat tccaaggtat accagtttat cggcgaggat
     2701 aatgtctact tttacgggcc ggtggaaatg gcaatgttca tggggaccca ggggaaggct
     2761 ccttccacca atcccccgga gggagaactg caactgccgg atctaatcgt caacaaccat
     2821 gtcctgtttt tgaataaaaa agccagcagc agcggggcga ttaaggcgcc gatggccaag
     2881 gatttgctgg attattatac ggcagaacaa ttaagggcgc acttccttgg cttgggactc
     2941 ggcatccgga gcgtgagttt tcagcctaaa ccgttaaacc ccatggcatc ggagaaagac
     3001 agcgatccgg ttttgaagga aggaaatttg ttgaccaatg tctttaaccg ggcggtgcga
     3061 tcgtgctttt atactgccca gaagtattat gacggtagaa ttccggtggg agaaatcggt
     3121 gaggagatac tcaaagaatc ggaaaagacc atcctggaat atgagagact catgtacaag
     3181 tgcgattttc atatcgtgat gaacctcatg gataccttca tccggaatat caataaatac
     3241 tggtctaaaa acatgcggga agctgaggag aataataatg gaacgctccg cctccagaca
     3301 ctggtggaca ccttccatat ggtccgcacc gcggcggtac tgatgcatcc gatcgcgcct
     3361 caaggtacgg agatgatcct tgactatctg aacctgggtg aggaattttg gaattgggat
     3421 cggatttttg agccgctcta cgccttcatg aaggatcccc aaaatcacaa gctcaagttc
     3481 ctcgaaccgc gggtggattt cttcaagaaa catcccagcc agatcgcgca gatgaatgag
     3541 tctggagatt aatttatttc tttgcgcata atagtggttg attccacaaa ttctttagtt
     3601 tatcaaaagg ccaaccaccc ctatcccctc cttgaggagg ggaattttgt gatattatgt
     3661 ggggacaccc cgcaccccgg cagcattgac atgctgcact tgtccttagc acttggatca
     3721 cagaattttt gatctttcat cgatgcacaa ctcccagttg aggatatagg tgaacagatg
     3781 aatggagatt gggcctatct gagcagggaa ccgctggtgc tccggagcaa tcgcgtctgg
     3841 cggacatatc aaggcggaat gaagatcgag gagtggcagg gaaagccgga tccgaaggat
     3901 ggcgactatc ccgaggagtg ggtggcctca accgttaaag cccgtaatcc tggccgcgaa
     3961 gcctatggcg acgaaggttt aagtaaacta aagattgatg gggaaacgac caccctaaag
     4021 cagctgatcg actccgaccc gggcgctttt ctcggggaga agcatgtcca aaaatacggc
     4081 agtaatatgg cgctattatt taaggtgctc gattccaaat gccgcttgtc gattcaggtt
     4141 catcccgacc ggaaattctc caaccaggtt tttaactctc cgtttggtaa gacggaggcc
     4201 tggtttgtgt tggggagccg ggagattagt caaacggagt cttatttgtt atttggattt
     4261 aaaccgggag tcaccaggga aagatggcgg gaactgtttg aacggcagga tatcgaggga
     4321 atgatcgatt gcctgcataa gattccggta attcccggag aagtttggtt gattgagggg
     4381 ggactacccc acgccatcgg accgggttgt ttcttaattg aagtccagga gccgacggac
     4441 tacacgattc gggtggaacg aaccacgatt gacgggcgta aactcccgga tgtcctgtgt
     4501 caccagggag ccggtttcga taaaattttc gattgttttc attacgacga atcatgttca
     4561 tatgaagaaa cttttaagaa atggcggatc accccgcgac cggttcaatc ggttccaggg
     4621 gccgaagtaa tggaactggt ggggaaagcc gataccgata aattccggtt atacaagtat
     4681 gaggtgagcg gctcattaaa ctgccgtgac gaacgggttt attcgattgt gacggtgatt
     4741 gccggatcgg gaaggatttt gtatcaaaat gggcagactt tcatcggccg gggcgatcaa
     4801 ttgtttctcc ctgcaaattt aggcgatttt aagtggcagg ccgatccggg gtcgaaactg
     4861 gtgttggcgc gggcctatcc gcccgactga ctatacgttg tttccggaac tggatagtat
     4921 tcatgcccaa attttatggg cgaatgagga ggaaccagat tgagctgtta tattcgagtg
     4981 aaagacgcag aagtcgagca acaaggtccc tcaaagacct atctcttact ctcggacaaa
     5041 caaggcagtg tcaaaggttg ttgttccggg ttaaatctgc tgttgaacga ggagtacaat
     5101 acccccgggg tgcatgaaga tcaggaaggc ttctttgtga tcgagggaac cggttgggct
     5161 aaatttggag atgaggagtt ccgtctggag cctgatatgg cgatgctcgc ccccgccgga
     5221 gtggcgcact gcatgaaaaa agatcccgga gccaaagtgt taaaggttct ttggtttcat
     5281 gcggcaattt aagaatttat gtatcagatg tattcggcgt caagaaattc ggaatcttaa
     5341 caggacccat aaaaacagac attgcaaaag atcccctttt gaactccggc gtttcaagat
     5401 gtctaatagc aactcacccc ctcgatcccc ctctcttttc ggcttaacat tacccttaag
     5461 ttggaggaac ttggataaat tagatcaatt cagtaaa
//
LOCUS       DASVMB010000037         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_666945,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000037 DASVMB010000000
VERSION     DASVMB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666945"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            617..1657
                     /gene="argC"
                     /locus_tag="VF531_01200"
     CDS             617..1657
                     /gene="argC"
                     /locus_tag="VF531_01200"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7712610.1"
                     /translation="MIQIGIIGASGYTGGELIRILALHPKVEIAAITSRTHEGKKLEE
                     VFSQFVGWNGPVFSGSDSPEAVKECDLVFLAVPHGVAMQIAPELLAKGQKVIDLGADF
                     RFRAPETFTAWYHQEHSEPELTRNAIYGLPELYRKEIRDADIVGNPGCYPTSIILGTA
                     PFLRAGLVDPASVICDSMSGVSGAGRKADLNYHYPELFGNFKAYGVTTHRHTPEIEQE
                     LSLAAGTPLVISFTPHLLPVARGILSTIHMKLAQKLSTVQAEKVLTDAYQGEPFIKVL
                     KEPRLPELKGVVGTNCCQIAARVDERTGQLIVISAIDNMVKGAAGQAVQNMNLMCDLP
                     ETMALVNWPIYP"
     gene            1768..>2019
                     /locus_tag="VF531_01205"
     CDS             1768..>2019
                     /locus_tag="VF531_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX7712611.1"
                     /translation="MHSITGGVTAPQGFKAGGITCGIKKNQRPDLALVVSDNPAVAAG
                     IFTTNQVQAAPVQLSKEHLRGGVARAIIANSGNANACVGP"
BASE COUNT          491 a          463 c          585 g          480 t
ORIGIN      
        1 tcgtcaaaac tgtcgaaccg cctagtgagg tcccgcatgc taggtggtgt gagaggacgg
       61 gggctagccg ccccctccta ctcgatcatg atgaatgggc tattgatgtc aaattaaaat
      121 agaggcttcc gaggcggata gtatttgacc gcgaattcgc gaaagaaaga tgaagattcg
      181 cgtgcgcctt atctgttcaa tggttaaccg gtaaatggta aaaaatttgg acgagcccat
      241 tcaaaagggg caaattgtgg gattagaaag gaaatagacg atctatcgag gaaatattga
      301 ttagggtttg attgggcgaa agcgatgaat aaaaatccga gccggaatct agcactcgag
      361 aaggggccta tcccaatgta acacatattt taacaatgcc ggagggttct tgttttttat
      421 tccgtaactt tgtataatta tgaataatcc cgttgagagg caagtcgcgc atacgtgtct
      481 gtgtttccga cttggttcca aaaaccggcg gcttgggact aatgaaagcg gcggcttttg
      541 ggagaatatc cggggatgtg ttaaaaaagc ttttgctgcg ccaaactccg aataacattc
      601 aaagaggttg attgatatga ttcagatagg aatcatcggc gcttccggtt ataccggcgg
      661 ggaactcatt cgtattttgg ctcttcatcc caaggtggag attgccgcta tcacttcccg
      721 cacccatgag ggaaagaagt tggaggaggt tttctcgcaa tttgtcggtt ggaacggccc
      781 ggttttttcg ggatccgact ccccagaggc ggttaaagaa tgtgacctgg tttttttggc
      841 tgtgccgcat ggcgtggcga tgcaaatagc tcctgagtta ttggccaagg gacaaaaagt
      901 tatcgacctg ggagcggatt tccggtttcg cgctcccgaa acctttacgg catggtacca
      961 ccaggaacac agtgaaccgg agttgacccg taacgcgata tacgggttac cggagcttta
     1021 ccggaaagaa atccgcgatg ccgacattgt cgggaacccg ggctgttatc cgacgagcat
     1081 cattttagga acggctccat ttcttagggc gggcttggtt gatccggcct ctgtcatctg
     1141 cgattcgatg tcaggggtct ccggcgccgg acgcaaggcg gacctgaatt atcattatcc
     1201 cgaactattt ggtaacttta aggcatatgg agtaacgacc caccggcata ctccggagat
     1261 cgaacaggaa ctttctcttg ccgcagggac gccgctcgtg atctcgttca caccgcatct
     1321 gctgccggta gcccggggaa ttttgagcac gattcatatg aaacttgctc aaaagcttag
     1381 caccgtccag gcggagaagg tactgaccga tgcgtaccaa ggcgagccat tcattaaagt
     1441 attaaaggaa ccgcggttgc cggagttaaa aggggtagtg gggaccaatt gttgtcagat
     1501 cgcggccagg gtcgatgagc gaaccgggca gttgatcgtg atatctgcca tcgacaatat
     1561 ggtgaaggga gcggccggtc aggcggtgca gaatatgaat ctcatgtgcg acctgcccga
     1621 aacgatggcc ctggtcaatt ggcctattta tccatagccc tcaccctgct ctcccccgtg
     1681 gatgcgtgaa atgggagtat ggcagcgggg ggagggtagg gggtgtgggc ctactcttta
     1741 caattttttt gagcgaggga tatcgagatg cattcaatca caggcggggt cacggcgccc
     1801 caagggttta aagcgggtgg aattacctgc ggaatcaaaa agaaccagcg accggatctg
     1861 gcattggttg tttcggacaa tccggcggtt gcggccggga tcttcaccac caatcaggtt
     1921 caagcggctc cggtgcaact ttccaaggaa cacctgcggg gtggcgttgc ccgggccatc
     1981 atcgccaaca gcggtaacgc taatgcctgt gtggggccg
//
LOCUS       DASVMB010000038         7833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_3852,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000038 DASVMB010000000
VERSION     DASVMB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="rsgA"
                     /locus_tag="VF531_01210"
     CDS             <1..770
                     /gene="rsgA"
                     /locus_tag="VF531_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX7712612.1"
                     /translation="EFSLIASDETKESPRGIITAVLPRETVLKRPYIANVNLLILVFA
                     HKNPDPSPRLITKFLVLAEASGIPFQVIFNKTDLVSRSRAEQLAASYRACGYPVMSTS
                     TLTHLGRMRLQATIKDKVTVFAGPSGVGKSALVNMVAPGLKLVTGSVSSKIGRGRHTT
                     REVQLLTVKRGSYLADTPGFSQIDLDFIEPPMLAGFFPEFRPYLNQCKFATCLHRNEP
                     RCAITAALTAGAISPERYQAYLDLLEEIQAAWRNRYR"
     gene            822..1478
                     /gene="rpe"
                     /locus_tag="VF531_01215"
     CDS             822..1478
                     /gene="rpe"
                     /locus_tag="VF531_01215"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003342780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7712613.1"
                     /translation="MPEIAPSILNADLVNLKNAVAQIKQADWLHFDVMDGHFVPNLTF
                     GPMFVGAIRKVSELPVEAHLMVENPEFFIPLYAAEGSRRLIIHAETTQHLHRIVQQIK
                     ESGCEAGIALNPATPLNCVDYILADLDLVLLMTVNPGYGGQKLIPEVIPKIRELRRKI
                     DTIGLACKIEVDGGVNWENASVLLDAGVDILVAGSLIYHDPDPTGALERLQGICRINR
                     "
     gene            1599..2069
                     /locus_tag="VF531_01220"
     CDS             1599..2069
                     /locus_tag="VF531_01220"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEX7712614.1"
                     /translation="MSFEHIDAFVKAASTVIGNMLNPQVEAGKPFFNDEPLKKYEVAV
                     LVGVLGDLQGQVICGMSETTAKAIISQMMGTEVQGIDQMGASAICELKNIIVGTASTN
                     LSLKGYRCNITPPLFIKGGTIPEFLKHIHTALAIPIQGPFGAIDINLALRKDEL"
     gene            2143..2409
                     /locus_tag="VF531_01225"
     CDS             2143..2409
                     /locus_tag="VF531_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEX7712615.1"
                     /translation="MAESTEVTRRFSAKDENISEVGLVLREVCMALKEKGYDPLDQIV
                     GYLLSGDPAYITSYRNARVLIKRLDRDQILEELVRSYLVKSGIG"
     gene            2730..3188
                     /locus_tag="VF531_01230"
     CDS             2730..3188
                     /locus_tag="VF531_01230"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7712616.1"
                     /translation="MSTYLGTELTSTLKDLFDQRISTVIVATLDRDLHPHTAPFHYIV
                     VCNPGRLRLAVSKELLTYRHIADNAYVALAVLEEGDVAVCIKGSARVVLPKMDCDCNM
                     AVVEVEIDEIRRDNSQSHYVTQGIRIRHRDEAHLLEARKIFLELTRSCQS"
     gene            3228..4181
                     /locus_tag="VF531_01235"
     CDS             3228..4181
                     /locus_tag="VF531_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712617.1"
                     /translation="MRKVYSFVVAAWAFTMMGCFFVKQLPLPLPGIYPIKMKTLTYLD
                     LASKSLNDLDWNTAELTESRPEGLTVAFDYSTMKNVQFGRFQLGNREQKVWFLMAQDD
                     ANNWSEFYIDQNLDMQITAKERVKSIQSSGQDRYRGIDRQLELGLIPVSLEIPYKGSN
                     REFEQNLYFFIGINTFSKKDLSDIMVGLITASFLEGDFPVTSGRSSKSIRFRILDANG
                     NGCFNDYGTDLVFMDLNGDSYFEKNESQSLKEFFVNGSGARRRQYEMVVPPFPVKLGV
                     YPTDEEYDSIQLEPSSTPSSPEVTQKSGTAQNGAKPGGPKE"
     gene            complement(4223..5068)
                     /locus_tag="VF531_01240"
     CDS             complement(4223..5068)
                     /locus_tag="VF531_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7712618.1"
                     /translation="MNQENKETSIPDVGVEENRLRDDEVWEGVLTQPIRGRNPKPRSV
                     GITMLIDKGLTISETRGLLELNAAAIDFIKLTFGTAALYPRQILMEKIELAKAYSVAV
                     YPGGTFFEIALWQGKTMTYLQKLRELGMEWVEISDGTLSISPQKRDAAIRMARDQGLR
                     VISEVGKKDSASQPEETVLVDTARHDFNSGADWAIIEGRESGKGIGIFDVDGGVIGPK
                     LTALREQLPAERIIWEAPLKSQQAKLINLLGTNVNLGNIPPEEIMALEALRRGLRSDT
                     WAKCL"
     gene            5209..5667
                     /locus_tag="VF531_01245"
     CDS             5209..5667
                     /locus_tag="VF531_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEX7712619.1"
                     /translation="MIQDNLILVIIFALGLAFNNNLVAAGAGSLLVLKLIKLRSLLLL
                     LERRAIDIGLLFLLVAVLVPFALDRVGVKEIWRTFQSVPGLVAIFGGVVAAYVCGQGV
                     VLLQVQPEVIVGLVIGTVIGVSLLKGIPVGPLAAAGVTSVILNLLGIGKK"
     gene            5710..5976
                     /locus_tag="VF531_01250"
     CDS             5710..5976
                     /locus_tag="VF531_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2619 domain-containing protein"
                     /protein_id="HEX7712620.1"
                     /translation="MERVILNNMALIRVISGMIEIVAAMIFLKVGRVDTALRLNAILG
                     LIGPLIFLAVSVLGIAAIAVKLSWTKVVLISLGIILVLIGTKSS"
     gene            complement(6151..7284)
                     /locus_tag="VF531_01255"
     CDS             complement(6151..7284)
                     /locus_tag="VF531_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712621.1"
                     /translation="MKKFWIGSIVFMLMIFMANLGVAATPTGDGYTSLNRKVNDLSNR
                     FSDLESNWGRFQIGGAFTLTPTANLYKQDPTLPELDFNQQLNLSLNAAIDPNLQFIMH
                     LAEQGSWGSTATPLSMPLWVDEAYLKMESANNLNYLGRFKFSLGPLGLISDFITDPAE
                     GAAVQLSENNFFMIGLYSRIFEDDYLAFRTGWSNRNQVVGINFVPNGITGEKSVSFDW
                     SASSSTTSLAAEFGLYSFTSDEHPDLNVSMAPGALISYGRLLPSKTFIQFKAGYFSPH
                     FTPIYSSLSYSSHYDREWFSSNSQGVEFFMQNRLKPDLQLENRLIALAPVENFNQPDL
                     GYRWRIDLAKNYSSLNQLQAGVDLKVLPGDISQQVFVQWNVRF"
     gene            complement(7412..7606)
                     /locus_tag="VF531_01260"
     CDS             complement(7412..7606)
                     /locus_tag="VF531_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712622.1"
                     /translation="MILTGTRPTIRKLRNDPLLTQVKGMVLRKTAVTSGLAGNGSFFC
                     QSWQQFVTLLCRDHPDTNRI"
BASE COUNT         1909 a         1834 c         2057 g         2033 t
ORIGIN      
        1 tggagttttc cctgatcgca agtgatgaga ccaaggaatc accacggggg atcattactg
       61 cggttttacc ccgggaaacg gtcctgaaac gtccctatat tgccaatgtc aatctactca
      121 tactggtttt cgctcataaa aatcccgatc ccagcccgcg actgattacc aagttcttgg
      181 tgcttgccga agctagcgga atacctttcc aggtgatatt caataaaacg gacctggtct
      241 cccggagccg cgccgagcag ctagccgcat cataccgggc ttgcggctac ccggtgatga
      301 gcaccagtac gctgacgcat ctaggccgta tgagattgca ggctacaata aaagataaag
      361 ttaccgtatt cgccggtccg tcgggtgtcg gcaaatcggc attggtcaat atggtcgcac
      421 ccggtcttaa gttggtaacc ggttccgtca gctccaagat tggccggggc cgccatacga
      481 cccgggaggt tcaactgtta acagtgaagc gcgggagtta tctggcagac accccgggtt
      541 tctcgcagat cgatttggat tttatcgagc ccccgatgct tgccggcttc ttcccggagt
      601 tccggccata tctcaatcag tgcaagttcg cgacttgcct gcatcgtaac gaaccgcgct
      661 gcgcgataac ggcagcgctg accgcggggg cgataagtcc ggaacggtat caggcttacc
      721 ttgatttact ggaagagatc caagcagcct ggagaaaccg ttatcggtag agcagttcgt
      781 gttgcaaaca ttagcgccat tacagcccag aggagattgg aatgcccgag atagcgcctt
      841 caattttaaa cgccgattta gtgaacttga aaaatgcggt ggcccagatt aagcaggctg
      901 attggttaca ttttgatgtg atggacgggc atttcgtccc caacctaacc ttcggcccga
      961 tgttcgtcgg agccatacgg aaggtatcgg aacttccggt tgaggcgcac ttgatggtgg
     1021 agaacccgga attctttatt ccgctttatg ccgcagaagg cagccgtaga ttgattatcc
     1081 acgctgaaac tacgcagcat ctccaccgca tagtccagca gataaaggaa tccggctgcg
     1141 aggccggtat cgcgcttaac ccagctactc cactgaattg cgtggattac attttggctg
     1201 atctggatct cgttttgttg atgacggtca atccgggtta tggagggcag aagttgatcc
     1261 ctgaagttat tccgaagatc agggagttgc ggcggaagat cgatactata gggttggcat
     1321 gcaagatcga ggtggacggc ggggtaaatt gggagaatgc atccgtatta cttgatgcgg
     1381 gagtggatat ccttgttgcc gggagtttaa tctatcacga tccggatccg accggggccc
     1441 tggaaagact tcaaggtatt tgccggatca atcgctaagc ggtaaactgt tttcttcccg
     1501 aacgcaggca acgtaagcaa ggtatcataa attgccgaaa atcgggtttc ctgcgcgcat
     1561 gcgcgcaatt aatcttggtt taagaggggg tcatgtaatt gtcatttgaa catatagatg
     1621 ccttcgtaaa ggctgcttca acagtcatcg ggaacatgct caatccgcag gtggaggcgg
     1681 gaaaaccttt cttcaatgat gaacccttaa aaaagtacga ggtggcggtg cttgtcgggg
     1741 tattgggcga tctacaaggc caggttattt gcgggatgtc cgaaaccacc gcaaaggcaa
     1801 tcatctccca gatgatgggc accgaagtac agggaattga tcagatgggg gccagcgcca
     1861 tctgcgaatt gaagaatatc attgtcggta cggcgagcac caatctttcg ctgaaggggt
     1921 atcgctgcaa tattaccccg ccgttattta taaaaggtgg aacgattccc gagtttttga
     1981 aacatatcca tacggctttg gcaatcccca ttcagggacc atttggggca atcgacatca
     2041 atctggctct acgaaaggat gagttataaa aagcccgcat cgagagccgg ctttggggca
     2101 tacttcccaa aaacaggtta ttttttgacg gaggtgaaaa tagtggctga atctaccgaa
     2161 gtcaccagaa ggttttccgc aaaagacgaa aatatcagtg aagtgggtct ggtcctgcgt
     2221 gaagtctgta tggcattgaa ggaaaagggt tatgatccgc tcgaccagat cgtaggttat
     2281 ttattatccg gagatccggc atatattaca agctaccgaa atgccagggt tttgattaag
     2341 cggttggata gggatcaaat tttggaagag ttggtccgtt cctatctggt aaagtcgggg
     2401 atcggttaag cgttcttgag gcccggttct aaaaagaact gtttttccat attgtaaatt
     2461 gggtgttgtc tgagggtttt tgttattcct tcgactttct gcgagcgttc aggttccttg
     2521 cgcttgaaaa ctcttggtgt aagaataatt ccctaaatca atccggatgc tgacatcaag
     2581 gtactttttt atccatgaat cgacctgcct tttacacccg ctctttcaag tgaaatcgtt
     2641 gcaagatccg aggctttcga taatatttct gtaatagctc cggggtctgt tttatcactt
     2701 gcaatctttg aaagagtggg gggaggcccg tgtcgactta tctgggaacc gaactgacct
     2761 cgacattaaa ggatcttttt gaccaaagga tcagtaccgt aatcgtcgca acattggatc
     2821 gggatctcca cccccatacc gcaccatttc attatatcgt tgtttgcaat cccggccgcc
     2881 ttcgcctggc tgtttccaag gagcttttaa cctatcgcca tatcgccgat aatgcctatg
     2941 tggccttggc cgtccttgaa gaaggggatg tggcggtgtg cattaaaggc tcggcgcggg
     3001 tggtgcttcc gaagatggac tgcgattgta atatggccgt tgtggaagtg gagatcgatg
     3061 agatacgacg ggataattct caaagccatt atgtaactca gggtatccgg atccgtcatc
     3121 gtgatgaggc ccatcttttg gaggcacgaa agatctttct ggagttgacc cggagctgtc
     3181 agagttagag agtatcccaa tcaaatccgg cgggcggggg taaaaagatg cgcaaagttt
     3241 attcgttcgt ggtggcggct tgggcattta ccatgatggg ctgttttttt gtgaaacaat
     3301 taccactgcc gcttcccggg atctatccca tcaagatgaa gaccttgact tatctcgatt
     3361 tggcgtctaa aagccttaac gatttggact ggaatactgc cgaattaacg gaatctaggc
     3421 ctgaagggct gacggtcgcc tttgactact caacaatgaa gaatgttcaa ttcggccggt
     3481 tccaactggg caaccgggaa caaaaagtct ggttcctgat ggcacaggat gacgcgaata
     3541 actggtccga attttatatc gatcaaaatc tggacatgca gatcaccgcg aaggaacggg
     3601 tcaaaagcat tcagtcgtcc ggacaggatc gttaccgggg tatcgatcgc cagcttgagt
     3661 tgggactcat tccggtttcg ctggagattc cttataaggg atccaatcgc gaatttgaac
     3721 aaaatcttta tttctttatt ggcatcaata ctttcagcaa gaaagaccta agtgatatca
     3781 tggtaggatt aattaccgcc agtttcctcg aaggcgattt tccggtaacc tcgggccgga
     3841 gttcgaaatc aatacggttc cggatcttgg acgcgaacgg taacggttgt tttaatgatt
     3901 acgggaccga tttggtcttt atggatttaa acggagatag ctatttcgaa aaaaatgaga
     3961 gtcagtcgct gaaggagttc tttgtcaatg gcagtggggc acgccgtcgg caatacgaga
     4021 tggtggtgcc gccttttcct gtcaaacttg gagtttatcc aacggacgaa gagtatgatt
     4081 ccattcagct tgaaccatcc tctaccccgt cttccccgga agtcacccag aaatcgggta
     4141 ccgcacaaaa cggagccaaa cctggcggtc ccaaagaata aaactgcgct cggtggcttc
     4201 aaataccgga tctccggtca cattacaggc atttagccca ggtatcgcta cgtaatcccc
     4261 tccgtaaggc ttccaaagcc atgatttctt cgggaggaat atttcccagg tttacattgg
     4321 tacccaatag attgattaat ttggcctgtt gactcttgag cggcgcttcc caaatgatgc
     4381 gttccgcagg cagttgttcc cgtaaggcag tcagtttagg accgatcacg cctccgtcca
     4441 cgtcgaagat tccaattccc ttacccgatt cacggccttc aataatagcc cagtcggcgc
     4501 cggaattaaa atcgtgccgc gccgtatcca ccagaacggt ttcctccggt tgactggctg
     4561 aatctttctt tccaacctcg ctgatgaccc gtaagccctg gtcccgcgcc atccggatgg
     4621 ctgcgtcccg tttttggggt gaaatcgaaa gtgtcccgtc ggagatctcc acccactcca
     4681 tccccaactc tcgtaatttt tggaggtaag tcatggtttt tccttgccaa agagcgatct
     4741 caaaaaaggt tccccccgga tacaccgcaa ccgaataggc cttggcgagc tcaatctttt
     4801 ccatcaggat ttgccgggga tatagcgcgg cggttccaaa agtcaacttg atgaagtcga
     4861 tggccgcggc gtttagttcg agtaatcccc gggtttcgga aatggtcaat cccttatcga
     4921 ttagcatggt aatcccgacg gaacggggtt tgggatttct gccgcggatt ggttgggtta
     4981 aaaccccttc ccatacttcg tcatcccgga gtcggttttc ttcaaccccc acatccggta
     5041 tgcttgtttc cttgttttcc tgattcaaat cgatccctcc tcgctctaat ctatgccctg
     5101 agcctaaagg aggttcacct cggccggtag attttgtctg acggatggag ccgcagaggt
     5161 atgaaccgtt ttgaggcgga atagatctag acaaaggagt gaccgccggt gatccaagat
     5221 aatttgatcc tcgtgatcat ttttgcgttg gggctcgctt tcaataacaa tctggttgcc
     5281 gctggtgcag gtagtctcct agtgttgaaa ttgattaaac tgcgtagtct gctgctatta
     5341 ttggaacgac gggcgatcga catcggcctg ctctttctgt tggtggcggt attggttccc
     5401 ttcgccttgg accgggtggg agtcaaggag atctggcgga ccttccagag tgtcccgggg
     5461 ctcgtcgcaa tcttcggcgg agtagttgca gcctacgttt gcggtcaagg ggtggtcctg
     5521 ctccaggtgc aaccggaagt gattgtgggt ctggtcatcg gtacggtgat cggggtatcg
     5581 ttgcttaaag gaattccagt cgggcctctg gccgcagccg gtgtgacttc cgtcattcta
     5641 aacctgttgg gaatcgggaa gaaatgatga agcgcccgcc tcagcggggg cgatcaaagg
     5701 agccaagcga tggaacgggt aattttgaat aatatggcat tgatccgggt gatctccggg
     5761 atgattgaga tcgtggctgc aatgatcttt ttgaaggtag ggcgggtgga taccgcccta
     5821 cgtctcaatg cgatattagg attaatcggt cccttgatct ttctggcagt cagcgtactc
     5881 ggaatcgcgg cgattgccgt caagttatcc tggaccaagg tggtgctgat ctccctaggc
     5941 attattttgg tgctgatagg aaccaaaagt tcataacgga atgaacaaaa aatatatttt
     6001 tcaacaacga aatacacaaa acacacgaaa atatattgtg gcttcctatg gtaatatcgt
     6061 accagatagt ttcagaaatc tttttcgtga aatttcgtgt atttcgtggt ttctcatacg
     6121 gtaaaatgtc ttaaagcctg catcaactgg ctaaaaccgg acattccatt gaacgaagac
     6181 ttgctgggag atatcacccg gcagaacttt gagatccact ccggcctgaa gttgattgag
     6241 tgaggagtaa ttcttggcca ggtcgatccg ccaacggtaa ccgaggtccg gctggttgaa
     6301 gttttcgacc ggtgccagtg caatcaggcg attctccagt tgcaagtccg gtttgagcct
     6361 gttttgcata aaaaactcca ctccctggga gttggagctg aaccattccc ggtcgtaatg
     6421 agaggaatag ctcagactgg aatagatcgg agtaaaatgg ggcgaaaaat aacccgcctt
     6481 gaattggatg aaggtcttac tgggcaagag ccggccgtaa ctgattaatg cgccgggcgc
     6541 catggaaacg ttgagatccg ggtgctcgtc cgaagtgaac gaatagagtc caaattccgc
     6601 cgccaggctc gtggtgctgg aactagcgga ccagtcgaaa ctcacagact tttccccggt
     6661 gatcccgttc ggaacgaaat ttatcccgac gacctgattc cggttggacc agcccgtacg
     6721 gaaagcaagg taatcgtctt caaagatgcg ggaatagagc ccgatcatga aaaagttatt
     6781 ctcacttaac tggactgccg ccccttccgc cgggtccgtg atgaaatcgg agattagccc
     6841 caggggacct aaagagaatt tgaatctgcc tagataattg agattattag cggattccat
     6901 ttttagataa gcctcgtcca cccagagcgg catcgacaaa ggagtggccg tcgatcccca
     6961 gctgccctgt tcggcaagat gcataataaa ctgcaaattc ggatcgatgg ctgcattcag
     7021 ggatagattc aattgctggt tgaaatccag ttccggtagt gtcggatctt gcttataaag
     7081 attcgccgtg ggagttaggg taaatgcacc accgatttga aatcgacccc agttggactc
     7141 caggtccgaa aaacggttgg agagatcatt gacctttcgg ttgagtgaag tgtagccatc
     7201 tccggtcggt gttgcggcca ccccgaggtt tgccatgaaa atcatcagca taaaaacgat
     7261 cgaaccaatc cagaattttt tcaatagtta gccctcctta acttgataag gtgccgtttt
     7321 acccaaaaac atgcacgata tgggaccccg tattcaatat ggggaaccca tatacggtat
     7381 ggataaaacg gcggtagcgg gaactttgtc tttaaattcg gttggtgtcc ggatgatccc
     7441 tgcaaagtag ggtgacaaat tgttgccagg actggcaaaa aaaactgccg ttaccggcga
     7501 gaccgctagt aacggctgtt ttgcggagaa ccattccctt tacttgggtc agtaacgggt
     7561 cattgcggag ttttcggatg gtgggccgcg ttcccgtgag gatcacggct cattgcctcg
     7621 aaccggcggg gcaaactttt aagggatttt gttcgattaa ggtccctgta aactttggcg
     7681 tgttcacgcc gaatcttttt tatatctcta atctatgttt ggctctctaa aaatgtgaat
     7741 ggttagggaa atatccctaa ccacgttgcg tcaataattc ctcggccttt tgaatcagcc
     7801 cccgaactat caacccagcc tcgcgagtgg tga
//
LOCUS       DASVMB010000039         9106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_3943,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000039 DASVMB010000000
VERSION     DASVMB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3943"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF531_01265"
     CDS             <1..508
                     /locus_tag="VF531_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7712623.1"
                     /translation="FTSTQAGVVGGLMIACGVLGSAIVPLLSDKFRNRSRFVLLAIAG
                     SIPGLIGLTFARSYWMLLVSGGVLGFFMLSTAPIGFQYGAEIAYPAPEGTSTGILMQM
                     GQISGIAFILAMDLFKSPQTGSMTASMVILIGLMIIGVLFCLGLHESELMGAKSPAEP
                     ELASAREE"
     gene            719..1729
                     /locus_tag="VF531_01270"
     CDS             719..1729
                     /locus_tag="VF531_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX7712624.1"
                     /translation="MKQHFLKRLVLMMMPVSLIFAVIFGNAVAAGGTGTELADVVRQH
                     GQLQVVGSQLCDARGVPIQLKGVSSHGLQWFPFSKNTLKNFISDWHISVVRAAMYTVE
                     GGYIGNESQMKQRVKELVDQAIEDGIYVIIDWHILSDNDPNIHKTEAQSFFAEMARTY
                     GNYPNVIYEICNEPNQVTWNRAIKPYAEYVIPAIRAVDPDNIIIVGTDTWSQGVDAPA
                     DNPLNFKQIMYALHFYAGTHTKWLRDRADAALAKGIALFVTEWGTTQASGSGGVYRDE
                     AQRWIDWMADQKISWCNWSVSNKSEDSAIFKPRVSLDGPWRDSDLTESGLWVKSKILS
                     EH"
     gene            complement(1992..3170)
                     /locus_tag="VF531_01275"
     CDS             complement(1992..3170)
                     /locus_tag="VF531_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7712625.1"
                     /translation="MNFNYYIPTKILFGPGRLNDLGTMKLPGKKAMLVISSGPSLKEN
                     GIVDRVLGLLSSAGVKTIVFDRIQPNPIKSHVMEGAALVKKEGCDFLIGLGGGSSIDS
                     AKSIAVMARNPGDYWDYISGGSGKGMPVQNGVMPVVAITTTAGTGTEADPWTVITKEE
                     TGEKIGFGTPETFPVISIVDPELMLTVPPKLTAFQGFDALFHAAEGYIANCATPVSEL
                     YSLKSIELLAKYLPIAVKDGGNLEARTMVALANTLAGMVESTSSCTSEHSMEHALSGF
                     KPELPHGAGLIMLSLAYFEFFTDKVPERMAAMAEAMGEPTGTLSPGEKKLAFVHVLQK
                     LQTACGVDQLKISDYGIKKEDLPGLARQARATMGGLFEMDRYRLSLEETIGIMQKAYR
                     "
     gene            3389..4147
                     /locus_tag="VF531_01280"
     CDS             3389..4147
                     /locus_tag="VF531_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7712626.1"
                     /translation="MSAIERRQQLLTMIESANSITVSELSRRLGVSAVTVRKDLEALE
                     DHGLVTRTYGGAILGQTSVGKPGVANGALPLSACSPVVAKAAGFIRPGQTIMLGEGEF
                     LTELADYLIGLENLTVLTVSVKIALKLMKSPAIDVILTGGILRARTMTMLGHLAERVI
                     RELDYDVSFSEVDGIDPVRGITSNNMVEANTAAAVLSNCKQAVIIAEASALGKVAPVQ
                     LSKITPGFIAIINGRPDTAAVRQLSQAGLKIIIA"
     gene            4700..5713
                     /locus_tag="VF531_01285"
     CDS             4700..5713
                     /locus_tag="VF531_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7712627.1"
                     /translation="MKKSVVFVVVAALLGLMIASAFVGAAKTLTVGVAIYKFDDTFMT
                     GVRNAITATAAEKKIKVDMVDSQNSQPTQNDKVDLFITKRVNALAINPVDRTAAGVII
                     DKAKKAKTPVVFFNREPLPEDMQKWNKVYYVGAKAEESGTIQGQILVEYWKSHAEADR
                     NKDGVMQYVMLKGEPGHQDAELRTKYSIKAIQDAGIKVEKVAEDTGMWDRVKGQEKMA
                     AFIAANGDKIEVVLANNDDMALGAIEALKAAGYFKEGKYMPVVGVDATAPGLKALEDG
                     TMLGTVLNDAKNQGKATLMLSYVLAQGKTPTLANTGYTITDGKYVWVPYKKILKSNMN
                     DAK"
     gene            6009..7517
                     /locus_tag="VF531_01290"
     CDS             6009..7517
                     /locus_tag="VF531_01290"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7712628.1"
                     /translation="MSEFLLEMNGISKEFPGVKALDNVTLKVRPGTVHALMGENGAGK
                     ATLMKCLFGLYKPDNGEIILNGEKIDITDPRCAMDRGISMIHQELNTVTFRNVMENVW
                     LGRFPMKSYGPIKLVDHKKMYQDTEKLFKSLDMDIKPKTIVGSMSVSKVQSMEIAKAV
                     SYDSKVIIMDEPTSSLTENEVEHLFRIIHELKDRGVAVIYISHKMEEILKIADEVTIM
                     RDGKRVGTWPSSELTTDMIISRMVGRDLTNRFPERQNVPGEVVLKVDGLTSPLPKSFK
                     EISFELRKGEILGIGGLVGAQRTELVEALFGLRRIESGKIILKGQPVQIKSPVDAKRH
                     KFALLTEERRVTGVISALSVMENMLVANVDHYVKKFLLNDKERKADAEKSVDLLRIKT
                     PSLKTIMKDLSGGNQQKVLFARWLLTEPDILLLDEPTRGIDVGAKYEIYTIIADLARQ
                     GKSIIMISSEMPELLGMSDRIMVMSQGRLSGIVDGQSTNQEEILRLAAKFIA"
     gene            7654..8649
                     /gene="mglC"
                     /locus_tag="VF531_01295"
     CDS             7654..8649
                     /gene="mglC"
                     /locus_tag="VF531_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HEX7712629.1"
                     /translation="MGLQKAGNFVSQHAIYFVLIALVGVMAFINPLFISFRSLNDILF
                     QSATRMIIALGAMFAILTAGADLSAGRMVGLAAVVSASMLQVPDYLRLFFPNLPQLPL
                     FVPILIAIIACTFFGLINGYIVSRLSVPPFIATLGTMVIIYGINSIYFDMPPNQSQPI
                     GGLRADFTNLGSGAIYSIPYIVIIAVIVTVLMWVVMNKTRFGKNTYAIGGNVNAARVS
                     GINVERTLLVMYVIAGALYGLAGVLESARTGGATNNYGNGYELDAIAACVVGGLSPTG
                     GTGTVQGIVAGVLILVIINYGLTFIGLNPYWQMIIKGLIIVSAVALDIRKSLAKK"
     gene            8876..>9106
                     /locus_tag="VF531_01300"
     CDS             8876..>9106
                     /locus_tag="VF531_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7712630.1"
                     /translation="MDRIILGRTNLKVSRSGFGAIPIQRIEFDDAKLVLRKAYEGGIN
                     FYDTARSYTNSEEKIGNALSDIRKDIIIATKTP"
BASE COUNT         2348 a         2069 c         2354 g         2335 t
ORIGIN      
        1 tttcacctca actcaggcgg gagtcgtcgg aggcttaatg atcgcctgcg gagtgctggg
       61 ctcggcaatt gtaccgttac tctcggacaa attccgcaac cgttcacgtt ttgtcctgct
      121 tgcaattgca ggatcaattc cgggcttgat cgggttgacc tttgccagga gttactggat
      181 gttattggtt tccggaggtg tgctgggatt ctttatgctc agtactgcgc cgatcggttt
      241 tcagtatggg gcggagattg cttatcctgc accggaaggc acttccaccg gcatattgat
      301 gcagatggga cagatttcgg gtatcgcttt cattttggcc atggatcttt ttaaatcacc
      361 gcaaaccggt tccatgaccg cttccatggt gatcttgatt gggttgatga ttattggggt
      421 tctattttgt ctgggacttc atgaatcgga acttatgggc gcaaaaagtc ctgccgaacc
      481 ggagttggcc tctgccagag aagaataatc cgccacaaga ctgaaaggca agaaaaaatc
      541 atcaaaaatt atagccgctt ctcccattga gaggagcggt attttttttg aaaagcaatt
      601 gatgcggggc tgtgaatggc aggaacagtt aggcaaattt ggcaattata agaggatttt
      661 ttgggaatcc acctaatttt ttacaatagg tttccaaata tgagggaggg atcggtaaat
      721 gaagcagcat ttcttaaagc ggctcgtttt aatgatgatg ccggtatcgc tcatttttgc
      781 tgtgattttc gggaacgccg tagccgccgg tggaacgggg actgaactcg cggatgttgt
      841 ccggcaacat ggccagcttc aagtggtagg gagtcagtta tgtgatgccc gaggagttcc
      901 gattcagtta aaaggggtga gctcccacgg actgcagtgg tttcctttct ccaagaatac
      961 cttgaaaaat ttcatatctg actggcatat ctccgtggtc cgcgccgcga tgtataccgt
     1021 ggagggcggt tatatcggca atgagtccca gatgaagcaa agagttaagg aactggtcga
     1081 ccaggccatc gaagacggga tttacgtcat cattgactgg catatcctga gcgataatga
     1141 tcccaacatt cataaaacgg aagcgcagag cttcttcgca gaaatggcga ggacttacgg
     1201 aaattatcct aatgtgatct atgagatctg taacgagcca aatcaagtaa cctggaatag
     1261 ggcaattaaa ccttacgccg aatatgtcat cccggccatc cgggctgtgg atcctgataa
     1321 catcatcatc gtcgggaccg acacctggag tcagggggtt gacgctccgg ccgacaatcc
     1381 tttgaacttt aaacagatca tgtatgcctt gcatttttat gcaggaaccc acaccaaatg
     1441 gttgcgtgac cgggccgacg ccgcacttgc caagggaatc gcgctctttg tgacagaatg
     1501 ggggaccacc caggcttcgg gaagcggcgg cgtttaccgg gatgaggcac agcggtggat
     1561 cgattggatg gcggatcaaa agatcagctg gtgtaactgg tcagtgtcca ataaatccga
     1621 ggatagcgca atttttaaac ccagagtgag cctggacggc ccatggcggg attcggacct
     1681 aaccgagtcc ggattatggg tcaagtcgaa aattctctcc gagcattaga atatccacca
     1741 tagcactgcc gggacaaagg acaggtagaa aactgaattt ataacaacga aaggcacgaa
     1801 tgacacgaaa taaagacatg gtatcatatc gttacggcag ctagcttagg aagttttcgt
     1861 gaatttcgtg tgtttcgtgg ttcgttatct atttaccgac gtaatagtca agaaaatgaa
     1921 ataaaaaagg atgcacaaaa tgtaggaaat aaacagaaaa gcaccggttg atatgaaccg
     1981 gtgctttaga tctaccgata cgctttttgc atgatcccga tggtttcctc cagcgacaat
     2041 cggtagcggt ccatttcgaa aagacctccc atcgttgccc gggcctggcg agcaagcccg
     2101 ggaaggtctt ccttcttgat accgtagtcg gagatcttta gctgatcgac gccgcacgcg
     2161 gtctgcaact tttgaagcac atgaacaaag gccagctttt tttctccggg ggagagtgtt
     2221 ccggtcggtt ctcccattgc ctcggccatg gccgccattc tttccggtac tttgtcggta
     2281 aagaactcga aatacgccag ggaaagcatg ataagtcccg cgccatgcgg caactcgggt
     2341 ttaaaaccac ttaaggcgtg ctccattgaa tgctccgaag tgcaggagga agtagattcc
     2401 accattccgg ccagggtatt ggcgagcgcc accatggtcc gggcttcgag attgccccca
     2461 tctttcacgg cgatgggaag atatttggcc aaaagttcga tacttttcag cgagtatagt
     2521 tcgcttacgg gagtggcgca gttcgcaatg tagccttccg ccgcatggaa caaggcgtca
     2581 aatccttgaa aggcagtgag tttgggcggg accgtcaaca tgagctccgg atcgacaatc
     2641 gatatgaccg gaaaggtttc aggagtgccg aaaccgatct tctctccggt ttcctctttg
     2701 gttatcaccg tccatgggtc cgcctcagtg ccggtaccgg cggtagtggt gattgcgacg
     2761 accggcatta ctccgttttg gaccggcata cccttgccgc tgccgccgct gatatagtcc
     2821 caataatctc cgggattacg ggccataacc gcgatggatt ttgcggagtc gatggaactc
     2881 ccgccgccaa gcccgatcaa aaaatcgcat ccttcttttt tgacgagggc agcgccttcc
     2941 atgacatggg attttatggg atttggctgg atcctgtcga aaacgatcgt tttcaccccc
     3001 gccgaactga gtagcccgag gactcgatcc acaataccgt tttcctttaa tgatggtccg
     3061 gaggagatca ccagcatcgc tttttttccc ggaagtttca tggttcccag atcattcagt
     3121 cgcccgggac cgaataaaat tttcgtaggt atgtagtaat tgaaattcac cgaaaaatca
     3181 ctccctcaat gcgattcccg gtctaggacc ggttttttta gtcttcccat agggtgaaca
     3241 gaatgtattg aacctatcaa atcacctgaa ttatgtcata atgatttgcc atcaagatac
     3301 agttttctat ttctctggaa ggaaaaatcc ttttcaagtc gaaagaagta ataaatagtt
     3361 gaaagttttt tccgtggagg gaaatcaggt gtccgcgata gagcgacggc agcagctgct
     3421 gacgatgatt gaatccgcaa atagtattac tgtatccgaa ctgagcaggc gactcggagt
     3481 ttcagcagta acggttcgaa aggatctgga ggcccttgaa gatcacgggt tggttacccg
     3541 gacctacggt ggcgcaatcc tgggacagac aagtgtaggg aagccgggtg tggcaaatgg
     3601 agctttgccg ctttcggctt gctcgccggt agttgccaaa gcggccgggt ttatccggcc
     3661 cggccagacc atcatgctgg gtgagggtga atttcttacg gagcttgcgg attatttgat
     3721 tggtttggaa aatctcaccg tacttacggt ttcggtgaag attgccttga aattgatgaa
     3781 atccccggcg attgatgtaa ttcttaccgg ggggattttg cgggcccgta ctatgacaat
     3841 gctgggtcat ctggcggaac gggtcatccg ggagctggac tacgatgttt cattctcgga
     3901 ggtggacggc attgatccgg tacggggcat aaccagtaat aatatggttg aagccaatac
     3961 cgccgccgcg gtgctcagca actgcaaaca ggcggtgatc atcgccgagg cctcggcatt
     4021 gggtaaagtt gctccggtac agctctccaa gatcaccccc ggcttcatcg caatcatcaa
     4081 cggacgaccc gatacggcag cggttcgcca actctcccaa gccggtctga aaattattat
     4141 tgcatgatac atggccaagc cctggccccc cggtctcaag cggtaaaggt cctcaaggat
     4201 gactatttcc agataactgt tattttctag gttgtctatg catatgcata attaatatga
     4261 aaaatgaact tcgcgaatac tagggctcgt tcattttgtt cttctgaaag tcccgtttga
     4321 agggactttt atcaggatta gtaagaaaag agaaccagac gaaagtaaac ctccggttaa
     4381 ccacagaaat gcccgatatg caggaaatca ttgaatcatc tggctttgcc tcgcatagaa
     4441 aaatattttg aaattgttaa aaggaaagca ggaaatttgt gtgaaggcag cgaaattatc
     4501 acattgtttc atctgctttc aacttagaac tttttctcct taccactttt tagattttcc
     4561 tttcgtaatg gaagactcct tttaaacgag ggtgtcggac gtttcgtgaa ggaaaacggg
     4621 ggaaaaattt gagatgaggc cgatgagacc ccatacacta tgagcgcggg ctcataaaaa
     4681 tttataggag ggattgtttg tgaaaaagtc ggtagtattc gtcgtcgtgg ctgcactgct
     4741 gggtctgatg atcgcaagcg cttttgtcgg cgctgcgaaa acattgaccg tcggtgtcgc
     4801 aatctacaag tttgacgaca ccttcatgac cggtgtgcgc aatgccatta ccgccacagc
     4861 cgctgagaag aaaatcaagg tggatatggt tgacagccaa aactcccaac cgacccagaa
     4921 cgataaggtc gacttgttca tcaccaaacg ggttaatgct ctggccatca acccggttga
     4981 tcgcaccgct gcaggcgtaa tcatcgacaa ggccaagaaa gccaagaccc cggttgtatt
     5041 cttcaaccgt gagccgctac cggaagatat gcaaaaatgg aacaaggttt actatgtagg
     5101 cgccaaagcc gaggagtccg gaaccattca aggccagatc ctggttgagt actggaagtc
     5161 tcatgccgaa gcggatcgga acaaagatgg cgttatgcag tatgttatgt tgaaaggcga
     5221 acccggccat caagacgccg agttacgtac caagtattcc atcaaagcca tccaagatgc
     5281 cggtatcaaa gtagagaaag ttgctgaaga cactggtatg tgggatcgtg taaaaggtca
     5341 agagaaaatg gccgccttca tcgccgccaa cggtgacaag atcgaagtcg tactcgccaa
     5401 taacgacgat atggctctcg gcgccatcga agccctgaaa gccgccggtt atttcaaaga
     5461 gggcaaatac atgccggttg tcggcgttga tgctaccgcc cctggcctga aagctctgga
     5521 agacggcacc atgctcggta ctgttttgaa cgatgccaag aaccaaggca aagcgacctt
     5581 aatgctctcc tatgttttgg ctcaaggcaa gaccccgact ctggccaata ccggttatac
     5641 catcaccgac ggcaaatacg tctgggtacc gtataagaag attcttaaat ccaacatgaa
     5701 cgacgcgaag taagctgatt ttcgcttaat agtttgaaaa agcaggggag cagttctgct
     5761 cccttgcttt atgatattta ggtcgtatgc gatatttagg ccatggcgga ttgaaacccg
     5821 acgaattcat cctctgattc cgtggaccgg ttttaccgcc gactagagta aattcacgtt
     5881 tgggcagaga acctccgcag tgaatgtata ttagcaattt ggtagttagt gcagaacgtt
     5941 aaattccgaa tccaaacggt aggatgaaac atagatccgt catgatcggg accgcagggg
     6001 gtgtaaaaat gagtgagttt ttacttgaaa tgaatggcat ttcaaaggaa ttccccggtg
     6061 tcaaggcgct tgataatgtt actctgaaag tacgccccgg cactgtccat gccctcatgg
     6121 gagagaacgg agccggtaaa gcaaccttaa tgaaatgtct ttttggcctc tataaaccgg
     6181 ataacggcga aatcatctta aacggagaaa agatcgatat caccgatccc aggtgcgcga
     6241 tggatcgcgg aatttccatg atccatcagg aactgaacac ggttaccttt cgaaatgtaa
     6301 tggaaaatgt ttggttaggc cgattcccga tgaagagtta cgggccgata aagctggtcg
     6361 atcataaaaa gatgtatcag gacacggaga agctctttaa gagccttgat atggatatca
     6421 agcctaaaac gattgtcggt agtatgtcgg tatcgaaagt ccaatcgatg gagatcgcca
     6481 aagccgtatc ctatgattcg aaagtcatca tcatggacga accgacctct tccttgaccg
     6541 agaacgaagt ggaacatctg ttccgaataa ttcatgaatt aaaagaccgc ggtgttgcgg
     6601 ttatttatat ttcgcacaag atggaagaaa ttttgaagat cgccgatgaa gtgaccatta
     6661 tgcgggacgg taaacgggtc ggaacatggc cttcttcgga actgacaacc gatatgatca
     6721 tctcccgaat ggtcggcagg gatttgacca atcgtttccc ggagcggcag aatgttcccg
     6781 gagaggtggt actgaaagtc gatggcttaa cctcacccct tcccaaatcc tttaaggaga
     6841 tctcctttga gcttcgtaaa ggtgaaatcc tggggattgg cggactggtt ggcgcacaac
     6901 ggaccgaact ggttgaagcc ttgttcggac tacgccggat cgaatccggg aaaattatcc
     6961 tgaaaggtca acccgttcag attaaatccc cggtggatgc gaaacgccat aaatttgcct
     7021 tgttaactga ggagcgacgg gttaccggtg taatttcggc actttcagtg atggaaaata
     7081 tgttggtcgc taatgtcgac cattacgtga aaaagttttt attgaatgat aaagaacgta
     7141 aagccgacgc agagaagagc gttgatctgc tgcggattaa aacgccttca ttgaaaacca
     7201 tcatgaagga tctctccggc ggaaatcaac aaaaagtact ctttgcccgg tggcttttga
     7261 ccgagcccga tattttactg ttggatgagc cgacccgcgg gattgatgtg ggagccaaat
     7321 acgagatcta caccatcatc gccgatctgg cccgtcaagg taagagtatc attatgatct
     7381 cttcggaaat gccggagttg ctcggaatgt cggaccggat catggtgatg agccaggggc
     7441 ggctatccgg tattgtcgat ggtcagagca ccaatcagga agagattttg cgcctcgccg
     7501 caaagtttat tgcataatct ctgaaaaaaa cggtgcaaga aactttcccg gttcgcttgg
     7561 aggacagcct ttggttttga cggcacattt gaaggagggt ttcaatttat ggactcaaag
     7621 aatactgtta aagctgaagc tcagaatcaa ctgatgggct tacagaaagc aggtaatttc
     7681 gtaagtcagc atgccattta ttttgtgctt atcgctctag ttggtgttat ggcttttatc
     7741 aatccccttt tcatttcatt ccgaagtctc aatgatattt tgtttcagtc tgcgacccgg
     7801 atgattatcg cgctgggagc gatgtttgca attttgacag ccggcgccga tctttcagcc
     7861 ggccggatgg tgggactggc cgcggtcgtt tccgcttcca tgttgcaagt accggattat
     7921 ttacggttgt tcttcccgaa cttgccccag ttaccgttat tcgtcccgat tttgattgca
     7981 attattgcat gtaccttttt cggtttaatc aacgggtata tcgtatcccg gttgagcgta
     8041 ccgcccttta ttgccacatt gggtaccatg gttatcattt acggtatcaa ctccatttat
     8101 tttgatatgc cgcccaatca atcccagcct atcggcggtc tgcgggctga tttcaccaat
     8161 ctcggcagcg gggccattta ctccattccc tatattgtca ttattgcggt cattgttaca
     8221 gtgctcatgt gggtggttat gaacaagacc cgcttcggaa aaaacaccta tgccatcggc
     8281 ggtaatgtta atgccgcccg ggtatccggt atcaatgtcg agagaaccct tttggtgatg
     8341 tacgtcatcg ccggtgccct gtacggactg gccggagttc ttgaatccgc cagaaccggt
     8401 ggcgccacca acaactatgg taacggctat gagctcgacg ccatcgcggc ctgcgtcgtc
     8461 ggcggtctct ccccgacggg cggtaccgga accgttcaag ggattgtagc cggtgtgttg
     8521 atcctggtta ttatcaacta tggactgact ttcatcgggt tgaacccata ctggcagatg
     8581 attatcaagg gtttaatcat tgtgagcgcg gttgcgctcg atattcggaa atctttagcc
     8641 aagaaataac tccaaagtta aagtcgatac tttgagaaaa gaaccgagtt ccaaacggca
     8701 acccggttct ttttttgtac gcccggaacg ggcgtcgtca tcggcatgcg taagtaattt
     8761 tttttttatt aaccgggtct tggggaaaat gattgttgac cttgagcggg tcgggtataa
     8821 tgaaagtgat gtttaaactg atatttacgt ggcagcagag aggagcgatt cggttatgga
     8881 tcggattatt ttgggtcgta ccaacttaaa agtcagccgg agcgggtttg gcgccatacc
     8941 catccagcgt atcgaatttg atgatgcgaa actggttttg cgaaaagcat acgagggcgg
     9001 gatcaacttt tatgatactg cgcggagcta taccaacagt gaggaaaaga tcggaaatgc
     9061 gctttccgat attcgcaaag atatcattat cgccactaaa actccg
//
LOCUS       DASVMB010000040        12345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_151458,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000040 DASVMB010000000
VERSION     DASVMB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12345
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151458"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..642
                     /locus_tag="VF531_01305"
     CDS             265..642
                     /locus_tag="VF531_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX7712631.1"
                     /translation="MPEKLDSKNSTQLGNVNIAPNAIAMVAGITALQCFGVVGMASRN
                     IQDGIAELFTGKDNLTKGIEVIIEDERVEVDLYIIVEYGVRIQEVARNVIENVKYAIE
                     SQLGLEITLVNVIVQGVRTNNKK"
     gene            795..2435
                     /locus_tag="VF531_01310"
     CDS             795..2435
                     /locus_tag="VF531_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX7712632.1"
                     /translation="MQPDAIDSELLKQLITSGTSSLHVHKAEVDALNVFPVPDGDTGT
                     NMYLTFQSALRELNKNGSHRVADLLESAAYGALMGARGNSGVIVSQLFRGFVKALPKD
                     LDQLSKQDIVTGLNGSASLCYQAVRKPVEGTILTVIKEMAGFATANVDKELTLTEFAE
                     KIYQHACSVLAKTPDMLPVLKQAGVVDAGGQGLVMFAKGIVQALKGEAPDETASELIE
                     TPVKVTSVTSYTDSDSLPEEEINFQYCTEFILKGKSLDLESLKNSLNPHGDCLLVVGD
                     ENTAKIHIHTNNPGIILDFAVRMGEMFEININNMVEQSQQRLAKLASSEGNNRRIGIV
                     AIASGDGMDRIFRSLGVQCIVNGGQTMNPSIEDLSKAVSQLNTPEIIILPNNGNIILT
                     ANHVQDLVSTPVKVVPTHSVPEGLAAVMSFSENLSLDENVSRMTEKSEAVITGEITYS
                     VRSIKLGELDIEKGDIIGLVNGEIVTSGKKPEDVVLKALEKTSEAHSGLISIYYGSDV
                     TAPTAQELLNRAEELLPDAEIELYYGGQPLYYYLFSIE"
     gene            2872..3429
                     /gene="rsmD"
                     /locus_tag="VF531_01315"
     CDS             2872..3429
                     /gene="rsmD"
                     /locus_tag="VF531_01315"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7712633.1"
                     /translation="MRIIAGEYRGRQLKSPKHEGVRPTADRVREALFNILGNRVCDAA
                     FLDLFAGTGAVGIEALSRGAKSAIFCDANHASIRLIQENSSFISGVDFQIMHLSAADA
                     LRVFAAERLCFDLIFLDPPFQAGLLPDTLNAIVAGDLLQPDGILVVEHPRKMVLKTPA
                     GLLLDKTRHYGDISLSFFLRIQSPA"
     gene            3482..3991
                     /gene="coaD"
                     /locus_tag="VF531_01320"
     CDS             3482..3991
                     /gene="coaD"
                     /locus_tag="VF531_01320"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7712634.1"
                     /translation="MTFETPNPHKVKAICPGSFDPITNGHLDIIERTAQLFPEVVVGV
                     LQNPSKKPLFSPEERVDLIRSAVKHLPNVEVYCFTGLLVDFVRKMHGNLIVKGLRAIS
                     DFEIEFQMALGNKRQAPEIETMFMMTNNEYSYLSSSMIKELALFGGNIHELVPEEVEK
                     ALRRKFSLV"
     gene            4002..4454
                     /locus_tag="VF531_01325"
     CDS             4002..4454
                     /locus_tag="VF531_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712635.1"
                     /translation="MPKMNLLLLLDHLEKLGNSNFSLAGKVWIDKEELAELIKKIQIA
                     LPDEIKEAEMISREKERYLSQAQEEAKRILREAETYAERLVREDQIVTRAEEESRRIL
                     EEAKRTAADIETDAMQYANKILEQLEESLERTIRIVHKGREDLNQGKY"
     gene            4518..5249
                     /gene="cmk"
                     /locus_tag="VF531_01330"
     CDS             4518..5249
                     /gene="cmk"
                     /locus_tag="VF531_01330"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7712636.1"
                     /translation="MKNKIRIAIDGPAGSGKSTIAREAALKLGYTYIDTGAMYRAITL
                     KAKNCRVKVTDQMELTRLTEESKIELLYEPVPEGTQLRLILDGNDVSDAIRSPEITAN
                     VSAVAAVAGVRTALVRLQQHLAIGGGVVMDGRDIGTVVLPDAELKIFLTASVEERARR
                     RSSELSAKGLAVDPDEIKLQIEKRDKYDSSREIDPLHQAAEAVLLDTTGLPISAVVDQ
                     VIELAVRRGAELPSRQVPAGVNNGD"
     gene            5239..6096
                     /locus_tag="VF531_01335"
     CDS             5239..6096
                     /locus_tag="VF531_01335"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7712637.1"
                     /translation="MEIKKARTAGFCFGVRRAIEMVDAALEDKSTPLYSLGPLIHNPG
                     VVQRLKSRGLGLVRSIGEVPHGRVVIRSHGAGPDVYREAAARGLTLTDATCPFVKNVQ
                     QLAISLRDEGYQVIIYGQAEHAEVQGVLDSIAGQALVVDDFRKLDTAMIRARIQPKVG
                     MLSQTTQEISGFGRLVTELIPLTKELRVFNTICLATSERQAEAAELSRQVDLMVVVGG
                     RNSANTSRLTEISRKNCSRVFQVEEAAELNPQWLAGVEKIGVTAGASTPDEQIQEVIA
                     QIEFLGGKC"
     gene            6096..7298
                     /gene="rpsA"
                     /locus_tag="VF531_01340"
     CDS             6096..7298
                     /gene="rpsA"
                     /locus_tag="VF531_01340"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7712638.1"
                     /translation="MEEANENSKGTEIDTTVSEPTDYDWSLDRFKELNEGQIIDAKVV
                     LVRDDAAFVDIGGKSDLTIPLEDLTVQQAISAKDLVRVGDVIQVMVTRTGDEDKIRLS
                     RRLVEQEKVWLELEKSFEQKSSVKATVTAAIKGGLSVNVNGVKGFMPASQAALSYIKD
                     LAGLVGQEFPVQILEFDRAKHRVVVSRRVILEEERKRAEAEFFATIHENDRKSGTVTR
                     ITDFGAFVDLGSGIEGLIHISELSWERVKSVRDILKEGDRVEVLVTKIDPASKRISLS
                     LRQIQQHPWDLATQNLREGETVTGKVVRMESFGAFINLAPGVDGLAHISQISDKHIAK
                     PDEVLSAGQEVQAKIIKLDRANRKISLSLKEVLQDQHDEEMAGYLNDQDSGAVTQNLG
                     ELLKKETK"
     gene            7525..8217
                     /locus_tag="VF531_01345"
     CDS             7525..8217
                     /locus_tag="VF531_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HEX7712639.1"
                     /translation="MMSGIVLLIGVSILIFLGMAHRVLDRLYLSDRGGIIFIVALIAG
                     SFIDIPIWRNPYISLNIGGAILPVALAIYVLSRAGSGREWLRSLIGIVLTTGVLYGVN
                     KLYRFDTARGFIEPQYLWAIIAGVVAYIAGRSRRLAFIIATLGVLAMDIAHVIEVGVS
                     GLNVPTKIGGGGIFDTIIIAGILAVLLAEIIGESREALQGGPEREDRPEELREALDHP
                     EIDADPKKEERP"
     gene            8214..9371
                     /locus_tag="VF531_01350"
     CDS             8214..9371
                     /locus_tag="VF531_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HEX7712640.1"
                     /translation="MKRFLEIILWTGFLFTIAVNPGLAEDELDNGYYKLTDGSGRLIT
                     QTAHKMVVGDEYLNHDNNLYQVVRLQGHTAVAKLIRKNAMEASVLDECRAFLVSVFNG
                     EFWRAEVRQKGPIGIYHTHSDESYIPGDGTASKPANGGIFQVGDSLTRSFENQGIPVI
                     HDKTPHDPHDATAYDRSRRTAAKLLRNQPTTLLDVHRDAVPEQVYADTIGNKGVTKVQ
                     LVVGRQNPNFEANNTFAKQIKQIVDKKYPGFIKGIFYGHGKYNQDLGPRSILLEFGAH
                     TNSKASAERGAQIFAAAARQALYGPARNAAVSRGSSRSLFWIVLAAVGGVGLFLLINR
                     GSLKSLGREFTGAMGETEEDKTTSGSDTPRPESDREDHPEDPKPPEGTTGG"
     gene            9390..9887
                     /locus_tag="VF531_01355"
     CDS             9390..9887
                     /locus_tag="VF531_01355"
                     /inference="COORDINATES: protein motif:HMM:NF022827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HEX7712641.1"
                     /translation="MKIIYHCFGGSHASPTAAAIHLGLIPRNRLPGFKELQQVPYFDQ
                     LTWEHHGKLIYAGTDEWGNDILFLARRNQAKLVIGIIKEFIRLNGGNPDEYYFIDCMQ
                     GFNPFMVTGGYSSRGLGLVKLGRPVVTFGTILSFPILVGIVKRTIRQVGMEFDEVRWL
                     NARPN"
     gene            9903..10370
                     /locus_tag="VF531_01360"
     CDS             9903..10370
                     /locus_tag="VF531_01360"
                     /inference="COORDINATES: protein motif:HMM:NF022827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HEX7712642.1"
                     /translation="MAKIFYYCFAGVHSSVVAASIHLGKLPSDRVPSLRELTGMPAFD
                     RKSDNSLGTPYFFGRDEQDNEVYVLGLGGNPRFMLEVIAHFLQSRPGLDTLDWKFFDA
                     LSTINFLAKMGGFISQRLKGYALGRWITAAGIRGSYVRLVDLVTTVRRCNRLW"
     gene            10364..10933
                     /locus_tag="VF531_01365"
     CDS             10364..10933
                     /locus_tag="VF531_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HEX7712643.1"
                     /translation="MVKRRIIVITDGDRIAQKVVERVARNVGGRAISLSGGNPTPIDG
                     EKVAAAVKQAACDPVLVMVDDCGDCDQGKGEKVLKHLAEDPELEILGVVAVASNTGSV
                     KGVPVTASINRDGGVVSGPVDKNGRPEPHGHQKVEGDTVDILNQIQVPVIIGLGDLGK
                     MDDADLVEDGARITTRAVQEILQRSRIND"
     gene            11077..12006
                     /locus_tag="VF531_01370"
     CDS             11077..12006
                     /locus_tag="VF531_01370"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX7712644.1"
                     /translation="MIIGIPRALLYYWYGGVWEDFWHAAGAETLVSPPTDQMILTAGT
                     EVALDELCLPVKIFLGHLRYLNSRVDYVMLPHLIKVEADAYICPKFMGLPDLARQTVP
                     DIGSKLISVTAGQKSADFRTGLFHAAQELGLKVPKNFLPPKGRYIPDNPGQALVQSYE
                     KWSVPKSGLTVGLLGHPYCLYDPELNLNLLDFLSNQGLRFITPELMPASYLDVGAGKL
                     NKSLFWTIGRTQFDALEWMLNDPVMKIDGFIHLASFACGPEAVVGDLLERRIKAARKP
                     LLKLNFEEQSGEAGMITRLEAFLDLIHYQRRAC"
     gene            12000..>12345
                     /locus_tag="VF531_01375"
     CDS             12000..>12345
                     /locus_tag="VF531_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HEX7712645.1"
                     /translation="MLKVTFPHIGNSYIPFRALLNELGLEPVVPPPITEATISLGTKL
                     APEFACFPLKVNLGNYVEALRQGAELILMAGGVGPCRFGYYGELQREILRDAGYDTEF
                     LVLEAPKTHPREL"
BASE COUNT         3242 a         2699 c         3313 g         3091 t
ORIGIN      
        1 aggaaacaat gatacaatta atctttttag agtaaaccgt ttataataag ttttgcattg
       61 atactgctca aaaagtctgc gtggcagatc cgtctcgtgg tacaaattat aaaaaaaggg
      121 gttgtttttc gtataaacat ggtgtagatt aatgcaggaa attggtattt catctcgaat
      181 tgtattccag gtcattttca gacttatttt tgaggattgg cgcgcacccg cgcgataaag
      241 tcgttgtacg ggggaggaag ctctgtgccg gaaaaattgg attccaaaaa ctccacccag
      301 ttgggtaatg tgaacatcgc acccaacgcc atcgccatgg tcgccggaat caccgccctg
      361 cagtgcttcg gggtagtggg aatggcctct cgtaatattc aagatgggat cgccgaactc
      421 ttcaccggaa aggataattt gaccaagggg attgaagtca tcatcgaaga tgagagggtt
      481 gaggtcgatc tttacatcat cgtcgaatat ggtgtacgaa ttcaagaagt ggcgcgtaat
      541 gtcattgaga atgttaaata tgccatcgaa agtcagctgg gactggagat tactctcgtt
      601 aacgtgattg ttcaaggggt acggaccaat aataaaaagt aatcaaagac ccggagggtt
      661 gtttctaaac gcgtgtttgc ggattttcca ttacggtatc taaactgagc catctagatc
      721 gtccgaaatt ggacgcaagt acttgaataa atcgtcggaa taccgtagaa tcaaagggtt
      781 aaggaggcag tatcttgcag ccagatgcaa ttgatagtga attattgaaa caattgataa
      841 catccggaac cagtagtttg catgttcata aagccgaggt tgacgccctg aatgtttttc
      901 cggttcccga cggtgatacc gggaccaata tgtatctcac atttcaatcg gctttacgag
      961 agttaaataa gaatggttcc caccgggtgg ccgatctttt ggagtctgcg gcttacggcg
     1021 cattgatggg agcccgcggc aacagcgggg tcattgtttc tcaacttttc agagggtttg
     1081 tcaaagcctt gccaaaggat ctggatcaac tttcaaaaca agatattgtt accgggttga
     1141 acggttctgc cagtctatgt taccaggcgg tcaggaaacc ggttgaaggg accattttaa
     1201 cggttatcaa ggaaatggcc ggttttgcaa cggcgaatgt cgataaagag ctaaccctca
     1261 cagaatttgc cgaaaaaatc tatcaacatg cttgttcagt gctggctaaa accccggata
     1321 tgcttccggt tttgaaacaa gccggagtag ttgacgctgg cggtcaaggc ctggtaatgt
     1381 ttgccaaggg tattgtccaa gcgctgaaag gcgaggcacc ggatgaaacc gcatcggaac
     1441 ttattgaaac tcccgtaaag gttacgagtg ttacatcata taccgatagc gattctcttc
     1501 ctgaagagga aatcaatttc caatattgca ccgagttcat tcttaaagga aaaagcctgg
     1561 atttggaatc actcaagaat agcttaaatc cgcacggtga ctgccttcta gtagtgggtg
     1621 acgagaacac ggcaaaaatt catattcaca cgaataatcc cgggattatt cttgattttg
     1681 ccgtgcgaat gggtgaaatg ttcgagatca acattaataa tatggtggaa cagagtcaac
     1741 agcgtctggc aaagctggct tcttccgagg gtaacaatcg ccggattggg attgttgcca
     1801 tagcgtcggg tgatggtatg gaccggatct ttcgcagcct gggggtgcaa tgtatcgtaa
     1861 acggcggtca aacaatgaac cctagcatcg aggacctctc gaaggctgta tcacaattga
     1921 atacccccga gatcatcatc ttgccgaaca atggaaacat cattcttacc gctaaccacg
     1981 tccaggattt ggtctcaaca ccggttaaag tagtgccgac ccatagtgta cccgagggtt
     2041 tggcggcggt tatgagtttt tcggagaatc tctcactgga tgagaacgta agccggatga
     2101 cggaaaagag cgaagcagtg attaccggtg agatcactta ttcagttcgg agtatcaaat
     2161 taggtgaatt ggatattgaa aagggcgata ttatcggttt agtgaacggg gagatcgtta
     2221 cttccggaaa gaaacccgaa gatgtagtgc tgaaagcact tgaaaaaact tccgaggctc
     2281 acagcggctt aatatctatt tattacggga gcgatgtcac agcccccacc gcacaggagt
     2341 tgttaaaccg tgctgaggag ttgttgcccg atgccgagat tgagctttac tacgggggac
     2401 aaccattata ctattacctg ttttcgattg agtgacgctc ccgccaggtt atagcttggc
     2461 gaaattgcct gtatattacc atgtaatatg acaggtcaaa aatgagcaag ctgtaattac
     2521 ggcgaactga ttaccttaca tattgaagac agttgtgaga cggatgattt gccaggaaac
     2581 agataccggg taattaagac ttacggcagt tttccgatgg tcgccgccgg aatggggaaa
     2641 gcctccctga ccgtttttga ggcgactttt gttttaaaaa ctttagttta aaaaaacttt
     2701 agtttaaaac cttggtttta aaaatccagt aatcaaaaaa ataatttttt attaaaaata
     2761 acctctcaaa atagaccttt cctatgaatg aattgaaatt gaacgaaccg gagaatatgg
     2821 catattgctc tccctagcgg agagtattat actgaagcat gagagtgtca aatgagaatc
     2881 attgccggtg aatacagggg ccgacaattg aaaagcccga aacatgaggg cgtaaggcct
     2941 acggccgatc gggtccggga agccctcttc aatatcttgg gcaatcgggt atgcgatgcc
     3001 gcttttcttg accttttcgc aggtaccgga gcggtaggca ttgaggctct ttccaggggc
     3061 gcgaaatctg cgatattctg tgatgcgaat cacgccagca ttcgtttgat tcaggagaac
     3121 tcgagcttca tatccggagt agactttcag atcatgcatc tttcagccgc tgatgcgttg
     3181 cgggtattcg ctgccgagcg tctttgcttt gatcttattt ttcttgatcc accgtttcag
     3241 gcggggctgc tcccggatac gctgaatgct attgtagccg gcgatttgct tcaaccggac
     3301 ggtattttag ttgtggagca tccccggaag atggttctaa agacaccggc aggtttattg
     3361 ctagataaaa cacggcatta cggtgatatt agcctcagtt tttttctgcg tatccaaagt
     3421 ccggcttgaa tcggaagata taccggaaaa ttcttttgta aaacaagaag gattggaaac
     3481 catgaccttc gaaactccta acccgcataa agttaaggca atctgcccgg gaagcttcga
     3541 tccgattacc aacggacatc tcgatataat cgaacgaacc gcacaacttt ttcccgaagt
     3601 ggtagtcgga gtattgcaaa atccaagcaa gaaaccgctt ttttcaccgg aggaacgggt
     3661 ggatctaatc cgttcggctg ttaaacacct cccgaatgta gaggtatact gtttcaccgg
     3721 tcttttggtg gattttgtcc ggaagatgca tggtaatctc atcgtgaagg gtttacgggc
     3781 catttctgat tttgagatcg agtttcaaat ggcgttgggt aataaaagac aagccccgga
     3841 gattgagacg atgtttatga tgaccaataa tgagtattcc taccttagtt ccagcatgat
     3901 aaaggaattg gccctattcg gcggtaatat tcatgaatta gtgccggagg aagtagagaa
     3961 agctctgcga cgcaaatttt cactggtata ggagcaaaag catgccgaag atgaatttgt
     4021 tgttattatt agaccatctg gagaagctgg gcaactcaaa tttttcgctg gcgggaaagg
     4081 tttggatcga taaggaggaa ctggcggagt tgatcaaaaa gattcagatt gctctgccgg
     4141 atgagattaa agaagccgaa atgatcagcc gcgaaaagga acgttatttg agtcaggccc
     4201 aggaagaggc aaaacgtatt ttacgcgaag cggagactta cgccgaacgt ttggtgcggg
     4261 aggatcagat tgtcacccgg gccgaagaag aatcacggcg tatccttgaa gaagccaaac
     4321 ggacggcggc agatattgaa accgatgcga tgcaatatgc caacaagatc ctggaacagt
     4381 tggaagagag tttggaaagg acaatacgga ttgttcataa aggacgggag gatctgaacc
     4441 agggtaagta ttgatctctt tggcagataa ccgataggtt tcagtcatca tagatatttg
     4501 catttgaacg gggaaaaatg aaaaacaaga tcagaatcgc catcgacggg ccggccggat
     4561 ccggcaagag caccatagcc agggaggctg ccctgaaact cgggtataca tatatcgata
     4621 ccggagccat gtaccgggct attaccctta aggcgaagaa ttgccgggtt aaggtgaccg
     4681 atcaaatgga attgacccgc ttgacggaag agtccaaaat agaacttttg tatgaaccgg
     4741 taccagaggg aacccaactt cgtctcattt tagacggaaa tgatgtcagc gatgccatcc
     4801 gcagccctga gattaccgcc aatgtttcgg ccgttgcggc tgtagctggt gtcaggactg
     4861 ccctggtccg tctgcaacaa catttggcta tcgggggcgg agtggtgatg gatggccggg
     4921 atatcggcac cgtggtttta cccgatgccg aactcaaaat ctttcttacg gcttcagtgg
     4981 aagaaagagc ccggcggcgc agttcggaac tgtctgccaa aggactggcc gtggatcccg
     5041 atgagatcaa gcttcaaatc gaaaagcggg ataaatacga cagcagccgt gagattgatc
     5101 ccttacacca ggccgcggag gccgttttat tggatacaac cggactgcca atctcagcgg
     5161 ttgtggacca agtgattgag ttagcggtga ggcgcggggc ggaattgccc tcaaggcagg
     5221 tcccggcagg ggtgaacaat ggagattaaa aaggcccgga ctgccggatt ttgctttgga
     5281 gtccggagag ccatcgaaat ggttgatgca gccttagagg ataagtctac tccactttat
     5341 agtctggggc cgttgattca taaccccggt gtggtgcaac gcttgaaatc ccgggggctc
     5401 ggtctggtaa gatccattgg ggaagttccc catggccgag tggtaattcg ttcacatggg
     5461 gccggacccg acgtttaccg ggaagcagcg gcgaggggtc taacccttac cgacgcaacc
     5521 tgtccatttg taaaaaatgt gcagcagctg gccatttccc ttcgcgatga aggttatcag
     5581 gtcattatct atggtcaggc tgaacatgcc gaggtccaag gtgtcttaga ttcaattgca
     5641 ggccaagcat tggtggttga tgacttccgg aagctcgaca cagcgatgat tcgcgcgcgc
     5701 attcaaccga aagttggtat gttgagccaa acaactcaag aaatctccgg gtttggacgt
     5761 ttagtcaccg aattgattcc tctcaccaag gaattgcgtg tttttaacac catttgtctg
     5821 gcaacttcgg agagacaggc tgaggcggca gagttaagcc gtcaagttga cttaatggtg
     5881 gtagtaggcg ggcggaattc cgccaatacc tcacgattga cggagatcag ccggaaaaac
     5941 tgttcccggg tcttccaggt agaggaagca gcggagttga acccccaatg gttggctgga
     6001 gttgagaaaa tcggcgttac tgcgggagcc tcaactccgg atgaacaaat acaagaagtg
     6061 attgctcaaa tcgaattttt aggaggtaag tgttagtgga agaggcgaat gagaattcga
     6121 aaggtacgga gattgatacg acagtatccg agccgacaga ttatgactgg tcgcttgatc
     6181 gattcaaaga gttaaatgaa ggtcaaatca tcgatgccaa agtcgtattg gtgcgcgatg
     6241 atgccgcttt tgttgatatc ggcggcaaat cggatttgac aatccccttg gaggatctga
     6301 cggttcagca agccatttcc gccaaggatt tggttcgggt cggagacgtg attcaggtga
     6361 tggttacccg gaccggtgat gaggataaaa ttcgcttatc acgccgtctg gtggaacagg
     6421 aaaaagtatg gcttgaactg gaaaagagct ttgaacaaaa atccagcgtc aaagcaacgg
     6481 ttacagcggc tatcaagggt ggattgagtg ttaatgtcaa cggggtcaag ggttttatgc
     6541 ccgcatcaca agccgcgtta agctacatta aagatctggc tggcctggtt gggcaagagt
     6601 tcccggtaca gattctcgaa tttgaccggg ccaaacatcg ggtagtggtc tccagacgtg
     6661 tgattctaga ggaagagcgg aaacgggccg aagccgaatt ttttgccacg attcacgaaa
     6721 atgatcgtaa atccggtact gttaccagga tcaccgattt tggcgccttt gtcgatcttg
     6781 gttccggaat tgaaggattg attcatatct ccgaattgag ctgggagcgg gtaaaatcgg
     6841 tccgcgatat cctcaaggaa ggtgatcggg tggaggtgct ggtgacgaag atcgatccgg
     6901 catccaagcg aatctcctta tcccttcggc aaattcagca acatccctgg gatctggcaa
     6961 ctcaaaattt acgggaggga gaaaccgtca ccggaaaagt cgtccggatg gagtccttcg
     7021 gcgcctttat caatctcgct cccggagtcg acggattggc ccatatctcc caaatctccg
     7081 acaaacatat cgccaaaccc gatgaagtcc tttctgccgg tcaggaagtc caggccaaga
     7141 tcattaagtt ggatcgggcc aaccgcaaaa tctcactcag tctgaaagaa gtactgcaag
     7201 atcaacatga cgaggaaatg gccggttacc tgaatgacca ggatagtggg gcagtaactc
     7261 aaaatttagg ggaactcctg aagaaagaga ccaaataatt tcggacgaac ctgacgctta
     7321 aaccttaacc aatatactaa tcttaccatc ccaaatccga tcgtttgatc ggattttttg
     7381 tttcctcata tcaaaccccc aaccgcaagg agtacccctt tgactttcag tttcctgtat
     7441 gatccaggaa cgccgctttt cccagtacca ttccagcata accgggagta tggccggaga
     7501 ctctattgga taaaggaggt atcaatgatg agcggtattg tgttgttaat cggcgtcagc
     7561 atcttgatat tcttgggaat ggcccataga gtgctggacc ggctttattt gagcgatcgt
     7621 ggcggcatta tctttattgt ggctttgatc gcaggtagtt tcatcgacat acccatttgg
     7681 cgtaatccct atatcagcct gaacattggc ggagccattc ttccggtggc tttagccatt
     7741 tatgttctga gccgggccgg ttcaggtagg gaatggttgc gttccctgat cgggatcgtc
     7801 ttaaccaccg gtgtattata tggtgtaaat aaactgtacc ggttcgatac cgcccggggc
     7861 tttatcgaac cacaatacct ttgggcgatc atcgcaggag tcgtcgctta tatcgccgga
     7921 cggtcccgcc gactggcatt catcatcgcc acccttggag tactggcaat ggatattgcc
     7981 catgtaatcg aggttggcgt gagtgggctc aatgtgccta caaagatcgg cggcggcggt
     8041 atcttcgata cgatcatcat cgccggaata ttggcggtgt tattggctga aataatcggt
     8101 gagagtcgtg aggcgcttca gggtggccct gagcgtgaag accgtcccga agagcttcgg
     8161 gaagcactgg atcacccgga gattgatgcg gatcccaaga aggaggaacg gccgtgaaac
     8221 ggtttttgga gatcatcctg tggaccggat tcctgtttac gatcgccgtt aaccccggtc
     8281 tggccgagga tgagttggat aatggttact ataagcttac cgacggttcg gggagattaa
     8341 ttacccagac tgcccataaa atggtagtcg gggatgagta tttgaatcat gacaataatt
     8401 tgtaccaggt cgttcggtta caggggcata cggcagtagc caagctgatt aggaaaaacg
     8461 ccatggaagc cagtgtttta gacgagtgtc gcgctttcct ggtttccgta ttcaacgggg
     8521 agttctggcg ggctgaggtc cgtcaaaaag ggcccatagg gatttatcat actcattccg
     8581 atgaatccta tatccctgga gacgggacag caagtaaacc ggcgaacggc gggatcttcc
     8641 aggtgggaga ctctctgacc cggtctttcg aaaatcaagg aattccggta attcatgata
     8701 agacccccca cgatccgcac gacgcgacgg cctatgaccg ttctcgacgt actgcggcaa
     8761 aactcctgcg caatcaacca accacccttt tggatgtcca tcgcgatgca gtgccggagc
     8821 aagtttatgc cgataccatc ggtaataaag gggtaaccaa agtccaactt gtagtgggga
     8881 ggcaaaatcc caattttgaa gcaaataaca cttttgccaa gcagattaaa cagattgtag
     8941 acaagaaata ccctggtttc attaaaggga tcttttacgg gcatggcaag tataatcagg
     9001 atctaggtcc gcgatcgata ttacttgagt ttggggcaca taccaacagt aaggcctccg
     9061 cggaacgggg ggcccagatc tttgcggcag ccgcccggca agcgctctat ggtcctgcac
     9121 gtaatgcagc ggtaagccgg ggcagttcgc ggagtctatt ctggattgtc ctggccgcag
     9181 ttggaggagt tggactgttt ttgctgatca accggggaag cctgaagagt ctgggccggg
     9241 agttcaccgg ggcaatgggc gagaccgagg aggacaagac taccagtggt tccgataccc
     9301 cgcggccgga atcggataga gaagaccacc ccgaggatcc caaacccccg gaaggaacca
     9361 cgggtggttg aggcccccca ggtaaaggct tgaagatcat ctatcactgt tttggcggtt
     9421 cccacgcctc acctaccgcg gcagcaattc atctgggttt gatcccccgc aatcgcctgc
     9481 ccggttttaa agagttacag caggtgcctt atttcgacca gttaacctgg gagcatcacg
     9541 gcaaactgat ttatgcggga accgatgaat ggggaaacga tatccttttt ctggctcgcc
     9601 ggaaccaagc gaagttggtt atcggtatca tcaaggagtt tattcggttg aacggcggta
     9661 acccggacga atattacttc atcgattgta tgcagggatt caatccgttt atggttaccg
     9721 gcggttactc ctcccgggga ctgggattgg ttaagctcgg ccggcctgta gttactttcg
     9781 gaacaattct ctcctttcca atccttgtcg ggatagtgaa gcgtaccatc cgccaagtcg
     9841 ggatggagtt tgatgaggta cgatggttga acgcccgtcc taattagcgc aaggaggact
     9901 gcttggcaaa gattttttac tattgttttg ccggggtaca ttcctcggtt gtcgcggctt
     9961 cgattcactt gggaaaacta cccagtgatc gggtgccctc ccttcgggag ttaaccggga
    10021 tgcccgcctt tgatcgtaag tcggacaaca gcttggggac cccgtatttt tttggccggg
    10081 atgaacagga taacgaggta tatgtgttgg gacttggcgg gaatccccga tttatgctgg
    10141 aggtcattgc tcatttttta cagagtcggc ctggcctgga tacgttggac tggaagttct
    10201 tcgatgccct gagcaccatc aatttcctgg ccaagatggg tgggtttatt tcacagcgcc
    10261 taaaaggtta tgccctgggt cggtggatta cagctgccgg aatccgcgga agttatgtgc
    10321 gcttggtcga tcttgtaaca acagtaagga gatgtaatcg gctttggtaa aacgtagaat
    10381 aatcgtgata accgatggag accggatcgc ccaaaaggtg gtggaaagag tcgcccggaa
    10441 tgtgggaggc cgggcgatct cactttcagg cggtaatccc acccctatag acggtgaaaa
    10501 ggttgccgcc gccgtcaaac aagccgcctg cgatccggtc ttggttatgg tagatgattg
    10561 cggtgactgc gatcaaggta aaggcgagaa agttttgaaa catctggcgg aagatcctga
    10621 attagagatt ttgggtgttg tggcggttgc ctctaacacc ggaagtgtaa agggggtacc
    10681 tgtcactgcc tcgatcaatc gggatggagg ggtggtatcc ggaccggtcg ataagaacgg
    10741 ccgacctgaa cctcacggtc atcaaaaagt tgaaggcgat accgtggata ttttaaatca
    10801 aatacaggta ccggtaatca tcggtctagg cgatctaggc aagatggatg acgctgatct
    10861 tgtggaggac ggagcgcgga ttaccactcg ggcggtccag gagatcttgc agcgaagccg
    10921 cattaatgat tgattcttcg agtattgatt aacaaaaaac aacaaacacc taacataatc
    10981 tgcaggaaaa gccttattta ttacgaaata tatagaatgt tttcttacaa ataaaaggta
    11041 agaaacatca agcgatcgcg ctgaggtgag aatggtttga tcatcggaat ccccagggct
    11101 ttactttatt attggtatgg cggggtctgg gaagatttct ggcacgctgc gggtgccgaa
    11161 accctggtct ctccacccac cgatcaaatg atcctaacag caggaaccga ggtggccttg
    11221 gatgaattgt gcctgccggt taaaattttc ttgggtcatc tacgttatct gaattcccgg
    11281 gtggattatg tcatgctgcc ccatctgatt aaggtggagg cagatgccta tatctgtccc
    11341 aaatttatgg gattacccga tttggcacgc caaacggtac cggatatcgg atcaaaactg
    11401 atatcagtga ctgccgggca gaaatctgcc gatttccgga ccggattgtt tcatgccgcc
    11461 caagaattag ggcttaaggt tccgaaaaac tttttacctc ccaaaggacg ttatatacct
    11521 gacaacccgg gacaagcatt ggttcaatca tacgagaagt ggtccgtacc aaaatcaggg
    11581 ttgactgtag ggcttttagg acacccttat tgtctgtacg atccggaatt gaacctgaac
    11641 ctgcttgact ttttatcaaa tcaaggatta cgttttatta cacccgagtt gatgccggca
    11701 tcatatctgg atgtgggtgc gggaaaacta aataaatccc ttttttggac catcgggcgt
    11761 acccaatttg atgctttgga atggatgctg aatgatccgg tcatgaagat tgacggtttt
    11821 attcatttgg cttcctttgc ctgtgggccg gaagcagtgg tcggcgattt gctggaacgg
    11881 agaattaaag cggctcgaaa accgcttctc aaattgaact ttgaagaaca aagcggggaa
    11941 gcgggaatga tcacaagact cgaggcattt ttggatctca ttcattacca gaggcgggca
    12001 tgttgaaggt aacctttccc catatcggca attcctatat tccctttcgg gcactcctta
    12061 atgaattggg ccttgaaccg gtagtgcccc cgcccattac tgaagcgacc atttcactgg
    12121 gaaccaaatt ggctccggaa ttcgcgtgct ttcccctcaa agtgaacttg ggtaactatg
    12181 tcgaggcact tcggcaaggc gcagaattga ttttgatggc cggaggggtc ggcccatgtc
    12241 gttttggcta ttacggagaa ctgcaaaggg aaattctgcg cgatgcgggt tacgatactg
    12301 agtttttggt actggaagcc cctaaaaccc atccccggga attat
//
LOCUS       DASVMB010000041         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_593475,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000041 DASVMB010000000
VERSION     DASVMB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593475"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VF531_01380"
     CDS             <1..764
                     /locus_tag="VF531_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7712646.1"
                     /translation="SRDRQAYSDQQAAIAEVLQQIKIGSGQQPAYPIEKEKELVLKIR
                     LGDKPGAQELLNEIFGYIFFSQGNEVETIKTRVIELVVVLSRAAMEGGADNEVIFGLN
                     YHYLREVNGLQTVDELAFWLSKVMARFTDSVFNLTNVKNSDILYKAVAYMRENYAQEI
                     KLEKLARHVNLSPTYFSKIFNEGMHCNFSNYLNVLRVENSKKYLLNEEIPLVEVAGLV
                     GFQDQSYYTKVFKRIINISPGEFRKSRGLIPRFPG"
     gene            853..1488
                     /locus_tag="VF531_01385"
     CDS             853..1488
                     /locus_tag="VF531_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX7712647.1"
                     /translation="MNTLEQISEYLQQGRAKNVQELVSQALTEGIAAGKILSQGLLAG
                     MSVIGEKFKVNEVYVPDVLIASRAMNAGMEVSKPHLMGSDIKPVGKVILGTVEGDLHD
                     IGKNLVKIMFQGAGFEVSDLGVDVSAEKFITAIKTERPQIIAMSALLTTTMINMDKVV
                     KAIVKEGLRDQVLIMAGGAPVTENFTQSIGADLYAPDAASAAQAARERLQH"
     gene            1721..3922
                     /locus_tag="VF531_01390"
     CDS             1721..3922
                     /locus_tag="VF531_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7712648.1"
                     /translation="MKWLSDTINRLGIRRLLQRIKQTKITPRTLWTAIQFEARTSTRY
                     RLIFFFLLVSLAPLIIVGLITFISSRSVIQSNITQFTIDSLVQVKVNLELKLKTYEDI
                     TQQFMSNQSANRLVEDYAKATTEIDQFSKIHDIQTYLLNYTVTDPNLGTEVISTSQRE
                     TYFGTGLTWKNGRNFKKSSIYRRITQSKGGAVWGTVSGNLSGNGRLVVVGRDISNLYT
                     GERLGVLAFFISENFIDQTINFNYYESDSGNKNDKNFYLVIDDQGRIVSAPLQYKGEI
                     GKSLGGLMGNPARLRSLLQGEITQGSFPGKFNHQQVLVTFKSLNNGWHLLSMAPASFL
                     VNTTRVVGLVVLILGLISGVLAVYISFKVAEAISRPLNQVMEAMKRAEDGDLTVRVAI
                     DSKDEFGKLGTSFNHMVAMISQLLVNTKGAINAVVRHSKDMAESSEQSATSSESVARA
                     MSDISKGTMEQTSETEKSSQQMTELANEIDSVVFKAAEVEMISDSTKALSFRSKDAVE
                     LLIIKSKETSEITNTIIKDIDELNSSAGQIRDITAMMEDIAEKTNLLAINAAIEAARA
                     GESGRGFAVVAKEVNKLAAQTQEAAQTIETILQTIQSKTNVSTQNADQAHVIVEDQMS
                     AVATAERAFDEIIAAMDDVVSRMGEMSKMVQQINNFKEQTLQSIVNISSISEETAASA
                     EEVTASTEEQTAIAGQVKQKAEELRLMAETLVESVERFQIADANEFDMKDR"
     gene            4135..5184
                     /gene="hrcA"
                     /locus_tag="VF531_01395"
     CDS             4135..5184
                     /gene="hrcA"
                     /locus_tag="VF531_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7712649.1"
                     /translation="MASKLDERKKRILKVVTDDYISSAEPVGSRTIARRYDLGLSPAT
                     IRNEMADLEEGGFLEQPHTSAGRVPSERGYRYYVDALMAVRQITDPELKSIYRELENY
                     HQEIESLIHQTSKILVQMTQYPSLILGPQVQTAIFRHIQLVKLSPNTVLVVIVTDTGY
                     VENKMIELEREVSTEELDRLSVLLNEKLRGLSLSDIRSGLLNEINSQLLFHDRFFNEA
                     IQLLLNAMASKSKERVFVDGATKILEQPEFDDLKKFKPLMNLLEEETRLYKLLSENLD
                     RKARVKIGHENQETAMQDCSVVTASYEIAGRTVGVIGVLGPTRMEYSKVLPIVENTAA
                     ILSELLTQMNKRRPR"
     gene            5239..>6619
                     /locus_tag="VF531_01400"
     CDS             5239..>6619
                     /locus_tag="VF531_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7712650.1"
                     /translation="MGDWMPMKEVMEETEVDQHLNALLTNSSAIQALGETVQGTLGPK
                     GLDCMLVDEYGGVVVTNDGITILKTMDLNHPAARLLLSAVENQEAQVGDGTTTTAILA
                     ATLVTAGTHQIMRGVPAIKVVEGIRYGIAKALKVLKTMVTPVTDLNSGILERIALVAG
                     RENHELAQLVIEAARALKGDKLNEPGFRLCDQVIALEGSESRLIPGTIVDREPLNRIM
                     PRHLTNAKILILDDALEPERLDAEALGTESGFNRMLHNDLEFKTDLLKLAELGIGAIF
                     TDRAISDEAEAVLTDLNILGVQGVARKEWLRLSEMTGARPLKRSALAKPLAELAGMAG
                     SAATISVDDQFKQIRVLATAEKPFVTVLTGALTKEVVCERERIAQDAAAAVQSAWLGG
                     VVPGGGSVELAISRILRQDAPQGVISYGYDCVVEALRKPMSQICLNAGFNPLEKSGEA
                     LLAQAEQNNF"
BASE COUNT         1787 a         1437 c         1769 g         1626 t
ORIGIN      
        1 tatccaggga tcgtcaggcc tactccgatc aacaggccgc cattgccgag gtactgcaac
       61 agataaaaat cggttccggg caacagccgg cctaccccat agaaaaagag aaggaattgg
      121 tacttaagat tcgcctggga gataaacccg gtgcacagga gttattaaac gagatctttg
      181 gttatatttt tttttcccag ggtaatgagg ttgaaaccat caaaacccgt gtgattgaat
      241 tggtcgtggt gctctcccgg gctgccatgg agggtggcgc agacaacgaa gtgatcttcg
      301 gcctgaacta tcactatctc cgggaggtca acggacttca aaccgttgac gaactggcct
      361 tttggctttc caaggtcatg gccaggttta ccgattccgt ctttaaccta actaacgtca
      421 agaacagtga tattctctat aaggccgtgg cctatatgcg ggagaattac gcacaggaaa
      481 tcaaacttga gaagttggct cgacatgtta atcttagtcc gacttatttt agtaagattt
      541 ttaatgaagg aatgcattgc aactttagca actacttgaa tgttttgcgg gtagaaaaca
      601 gcaagaaata tctgctcaac gaggagattc cccttgttga ggttgcgggg ttggtggggt
      661 ttcaggatca gagctattat acgaaagttt ttaagcgaat cataaatata tcccccggtg
      721 aattccgtaa atccagaggt ttgatcccaa gattcccggg atgatgcaag ctttagatga
      781 tggaggcttt atcggaaagt attgctccat ttataacata aataatggga tggttaaagg
      841 aggctgttaa tcttgaatac tttggaacag atttccgagt atttacagca gggcagggct
      901 aagaatgttc aagagctggt aagtcaggca ttaaccgagg ggatcgcggc aggaaagatt
      961 ctgtctcagg gtttattggc agggatgtct gttattgggg agaagtttaa ggttaatgag
     1021 gtatatgtgc cggatgtgtt gattgcctca agagctatga atgcaggaat ggaagtgtca
     1081 aagccgcatc tcatgggcag tgatatcaaa cccgtgggga aggtgattct cggcaccgtg
     1141 gagggggatc tccatgatat cgggaagaat ctggtgaaga tcatgtttca aggcgccggg
     1201 tttgaagtgt cggatcttgg cgtcgatgtc tcggcggaga aatttattac tgcgataaag
     1261 acggaacggc cgcaaatcat tgcgatgtca gcgttattga caacgacgat gattaatatg
     1321 gataaggtag ttaaggcgat tgtaaaggaa ggcttgcgcg accaagtgct gattatggcc
     1381 ggaggtgcgc cggttaccga aaacttcacc caatccatcg gagccgatct ctatgcgccg
     1441 gatgccgcat ccgccgccca ggctgcacgg gaaagattac aacattaatt gataggaaat
     1501 atgcttcttg attatcggtc gcgttgagct catcaacgcg attttttttg ccaaagaccc
     1561 catgagataa cctgtgatgc cggtcttttt ctggtgtatg gccaaatatt acaaagaaat
     1621 aacgaacgat gctaagatgg ttatttattc aataaacatg gtataattta cttgttattt
     1681 tctactagtt tatccgagtg ttttcggggg ggagtgcagt atgaaatggt tgagcgatac
     1741 cattaaccgc ctgggaattc ggcgattact tcaacgaata aaacagacga agatcactcc
     1801 aagaactctg tggaccgcta ttcaatttga agctcgcact tcaaccagat atcggttgat
     1861 cttctttttt cttttggttt ccctggcacc cttaatcatc gtcgggctga taacattcat
     1921 tagttcccgt tcggtaatcc aatccaatat cacgcagttc accatcgata gcctggtcca
     1981 agtcaaggta aatttggagc tgaagcttaa aacatatgaa gatataactc aacaattcat
     2041 gagcaaccaa agcgccaatc gattggtaga ggattatgcc aaagcgacga ctgagattga
     2101 tcaatttagt aaaattcacg acatacagac ttacttgttg aattatactg taaccgaccc
     2161 caacctcggc acggaagtaa tatccacttc acaacgggaa acatacttcg gcaccggtct
     2221 gacttggaaa aacggcagga attttaaaaa atcctcaatt tatcgcagga taacgcagag
     2281 caaggggggg gctgtttggg gcacagtctc cgggaatctc agcggtaacg ggcggttagt
     2341 ggtggtcgga cgggatatca gtaaccttta caccggagaa aggttgggcg tactcgcctt
     2401 cttcatcagc gagaatttca ttgatcaaac catcaatttt aattactatg aaagtgattc
     2461 gggaaataaa aatgataaaa acttttatct ggtgattgac gaccaaggcc ggatcgtttc
     2521 ggccccctta caatacaaag gtgagatcgg taaatcgctt ggcgggttaa tggggaatcc
     2581 agcccggctc cgctctttat tacagggtga aatcacccag ggaagtttcc ccgggaaatt
     2641 taaccatcaa caagtcctgg taacctttaa gtctttgaat aacggctggc atctcttgag
     2701 tatggcgcct gcatcgtttt tagtcaatac cacccgagtt gtgggactgg tcgttctgat
     2761 tctgggattg atctccgggg tgcttgcggt ttacatatcc tttaaggtgg ccgaagctat
     2821 ctcccggccg ctcaaccagg taatggaagc gatgaaacgt gctgaggacg gtgatcttac
     2881 tgttcgggtt gccattgatt ccaaggacga attcgggaag ttaggcacca gctttaatca
     2941 catggtggcg atgatcagcc aattacttgt aaataccaag ggagccatca acgccgtagt
     3001 gcgacacagc aaggatatgg cggagagctc ggaacagtcg gccacatcat ccgagagcgt
     3061 ggcgagagcc atgagtgata tcagcaaggg gaccatggag caaacctctg aaactgaaaa
     3121 atcctcacag caaatgaccg agctggcaaa tgagattgat agcgtggtat ttaaagcggc
     3181 agaggtcgag atgatctctg attctaccaa ggctttgagt tttcgatcca aagacgcagt
     3241 agaacttttg attatcaagt cgaaagagac gagcgagatt accaacacca tcattaagga
     3301 tatcgatgaa ctcaattcca gcgccggtca gattcgggac atcactgcaa tgatggagga
     3361 cattgccgaa aaaactaatc ttttggcaat aaatgccgca attgaggctg cccgggccgg
     3421 ggagtccggc cgggggttcg cggtggttgc caaagaggtt aataaactgg ctgcccagac
     3481 tcaggaagcg gcccaaacca tcgaaaccat ccttcagacc attcaatcca aaacgaatgt
     3541 ttcgacccaa aacgccgatc aagctcatgt catcgtggaa gatcaaatgt cggctgtggc
     3601 aaccgcggaa cgggcctttg acgagattat cgccgccatg gacgatgtgg tctcgcggat
     3661 gggcgaaatg agcaagatgg ttcagcagat aaacaatttc aaggaacaga ccctgcaatc
     3721 gatcgtcaat atcagctcga tctcggagga aaccgcggct tctgccgaag aagttaccgc
     3781 ctctaccgag gaacaaactg ccatcgcggg tcaagttaaa cagaaggctg aagagctacg
     3841 gttaatggcc gaaacgctgg ttgaaagcgt cgaacgcttc caaattgccg acgcgaatga
     3901 gtttgatatg aaagaccgat agggtcggtt gtggttcaag ggaaaaaccg ctatcaggcg
     3961 gtttttttat atctacacct tatcaagatc cagtttgtga aggccttcct aagtgttcgt
     4021 ctcgaattta cctgattccc cttgacaaac ggtaggagca gtgatatttt gttagtagat
     4081 tagcactcgc catagtagag tgctaacaat attaacgatc aagaggtgct tttcatggcc
     4141 tccaagcttg atgagagaaa aaagcgcatt ttaaaggttg taaccgatga ttatatctct
     4201 tcggcggaac cggtaggttc ccggacgatt gccaggagat atgatctggg actgtctccg
     4261 gccacgatcc gcaatgagat ggcggatctt gaggagggcg gttttttaga gcaaccacat
     4321 acctctgccg ggcgggttcc ttcggagcgg ggatatcgtt attatgtgga tgcgttgatg
     4381 gccgtccgcc agataacgga tccggaacta aagtcgatct atcgggaact cgagaattat
     4441 catcaagaga ttgaatcgtt gattcatcaa acctccaaaa tcttggtcca gatgacgcag
     4501 tacccctcac tgattctggg gccccaggtt caaacagcta tattccgtca tattcaattg
     4561 gttaagcttt cgcccaatac cgttttggtg gtaatcgtca ccgataccgg gtatgtggag
     4621 aacaagatga tcgaactgga gcgggaagtt tccacggagg aattagaccg gctctcggta
     4681 ttgctcaatg aaaagctgcg cgggctatcg ctttcggata ttcgctccgg tctactgaac
     4741 gagatcaatt cccagcttct ttttcatgat cgatttttta acgaagccat tcagctactt
     4801 ttaaatgcga tggccagcaa atccaaggaa cgggttttcg tggacggggc tacaaaaatc
     4861 ctggagcaac ccgagtttga cgatttgaag aagtttaaac cattgatgaa cctgttggaa
     4921 gaagagaccc gtctttacaa gctgctctcc gagaatctgg atcggaaagc ccgggtaaaa
     4981 ataggccatg aaaatcaaga aactgcaatg caggattgca gcgtggtgac tgccagttac
     5041 gagattgccg gccgcactgt cggagtgatt ggggtcttgg gtcccacccg gatggagtat
     5101 tccaaggtat tgccgatcgt cgaaaacacc gccgctattc tcagtgaatt gctgactcag
     5161 atgaataagc gccggccgcg atagattaaa atcaggcctg aggttataat aagcgttgat
     5221 tttggagctt accagcggat gggtgattgg atgccgatga aagaagttat ggaggaaacg
     5281 gaagtcgatc aacatctgaa tgccttgctt accaactcta gcgccattca agccctgggc
     5341 gaaacggttc agggaacgct cggaccgaag ggtctcgatt gcatgctggt cgatgagtac
     5401 ggcggagtag tcgtaaccaa tgacgggata accattttaa aaacgatgga ccttaaccac
     5461 cctgccgccc ggctactgct tagcgcggtg gaaaaccagg aggcacaggt tggggatggc
     5521 accactacca ccgcgatact tgctgcaaca ctggtaactg ccggaaccca tcagattatg
     5581 aggggagtcc ccgcaattaa ggtggttgaa gggattaggt acggcatagc caaggctttg
     5641 aaggttttga agacgatggt cactccggta accgatctga atagtggaat tctggagcgg
     5701 atcgcgctgg tggccgggag ggagaatcac gagctggcac agctggtgat tgaagcggcc
     5761 cgggctttga agggtgacaa gttaaatgaa cccggttttc gactttgcga tcaggtgatc
     5821 gcgctggaag ggtcggagag ccggcttatc ccgggaacta ttgtcgatcg ggaaccgtta
     5881 aacagaataa tgccccgcca cttaaccaat gcaaaaatcc ttattctgga tgatgcgctg
     5941 gaacccgaaa ggcttgacgc cgaagcgctt gggaccgagt ccggattcaa ccggatgtta
     6001 cataacgatc tggaatttaa gaccgacctt ttgaaactgg cggaattggg aattggggcg
     6061 atctttactg atcgcgccat ttccgacgag gctgaagcgg tcttgacgga tttgaacatc
     6121 ttgggtgtcc agggagtagc ccgtaaggaa tggttgagac tttcggaaat gaccggggcc
     6181 cgcccgttga aacggtcggc actcgccaag cccctcgcgg aattggccgg aatggccgga
     6241 agcgccgcaa ccatcagtgt cgatgatcag ttcaaacaaa ttcgggtact ggccacggcc
     6301 gaaaaaccgt ttgttacggt tttaacgggc gctttgacga aggaagttgt ttgtgaaaga
     6361 gagcgaattg cccaggacgc cgcggcagcg gtccaatccg cctggctggg aggcgtcgtt
     6421 cccggcggtg gcagtgttga gttggcgatt tcacgtatct tgcgtcaaga tgcgccacag
     6481 ggagtgatca gttacgggta tgattgtgtg gttgaagccc tacgtaaacc catgagtcaa
     6541 atctgtctta atgctggttt caatcctttg gagaaaagcg gtgaagcctt gctcgctcag
     6601 gcggaacaga acaattttt
//
LOCUS       DASVMB010000042        11494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_519708,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000042 DASVMB010000000
VERSION     DASVMB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519708"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VF531_01405"
     CDS             <1..813
                     /locus_tag="VF531_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7712651.1"
                     /translation="RHHPGRAIVWNVYLLGVGAFFLIPLGSILARSFHELGLPDTQLT
                     FTNYRLLFDSGFQFAVGASFGKVLATSLGLALVVAVLTVGCGYFFARSQRYRPWGSVD
                     FRFQLPLGISFLTFTTGLAVLAGNYLPPGILIVWAQIFLTFPLIYSMLRNACREVDPA
                     LTEAAASLGATRFVRLLTVELPLLKKPLVTAFAYAMALSLGDLAAVLSLGNGEVVTLS
                     VAIYRLIGHYRFAQANALGTIFIELAFGLFLVMEGYGSHFRNVWLKGDGYDD"
     gene            806..1885
                     /locus_tag="VF531_01410"
     CDS             806..1885
                     /locus_tag="VF531_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712652.1"
                     /translation="MTEPLLDLAVTKAYPDFQVQVRLNVAAGEFLALLGPSGCGKTTL
                     LRIIAGLERPDNGWVRLAGRDITFVEPAARRIGMVFQDYALFPHLTVAGNIEYGLKVK
                     KMAAADRSRRVGELLELFELNHLGNRSVNQLSGGERQRVALARALAPQPLILLLDEPF
                     AALDYGLRRRLRNELRELQLRLGFTVVFVTHQQEEALAVADRIGIMQAGMVLQTGPGN
                     EIYEHPANRYVAELLGDANLIPCRISGEDGQYILHDGNEVALPFQEKSGANQVTPHSA
                     KTGYLMIRPEDLILDPGLEYPAFTGTVKSLEYLGSTYQLTVVCSGGQMLQAFLSKQEG
                     CRIRRGQILRLGFSPQAARFLSDIS"
     gene            2066..2869
                     /locus_tag="VF531_01415"
     CDS             2066..2869
                     /locus_tag="VF531_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712653.1"
                     /translation="MRQTGRGPNPDLQKILSIWAAVLLIILVTACFSGIQVYYWQRSA
                     AAKERANLIRQNQRLAAEVARLKAALNHSGQGSNPGVQPRAISVKHPVAQGSSGKSPT
                     IPSNLAARERMVLEALRRQDMTALARLVDPEKGLRFSPYSYVNIQQDLVFDVDQLPGL
                     LRAKTNYLWGYYDGTGLPIRLKSAEYFKSFVYDRDYLLDGAEPSSGAVLKNSVTAGNV
                     FEVYPKAVVVEYRDAESKTSMNWHYLKLVFEARDKTWFLVGLVHDQWTI"
     gene            2974..5586
                     /gene="mutS"
                     /locus_tag="VF531_01420"
     CDS             2974..5586
                     /gene="mutS"
                     /locus_tag="VF531_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7712654.1"
                     /translation="MVKETPWVKQYLAIKKDYPDCLLFFRLGDFYEMFFEDAVMASRD
                     LELVLTGKNSGTERIPMCGIPYHAVSGYLAKLLAKGHKVAICEQMEDPKLAKGLVERA
                     VIRVVTPGTVIEDQLLKEKVNNYLAGVALDGQFWGLAYVDLSTGLFQTTQFPKAQIQI
                     LLNELTRIQPAELYLSETELQTLNQRPELADTLITTGPSDRFQTDRAYRCLTEHFQTQ
                     NLRSFGCEDRPAAIKAAGAVMAYLLETQKTSLEHIRKITSYEVGAYMTLDPATRRNLE
                     LVKTIRSNEVNGSLLWVLDLTVTSMGGRLLRSWIEQPLAGIEAINERQGGVAELTHRL
                     ELRESLREALSRTYDLQRILSKLATNSANARDLLALKNTLAEIPEIREFLSGVTAPKL
                     LMLLNALDPLADITRLLQESLLPDPPLTVKEGGMIRSGFNPELAKLLEAGTEGRQWVA
                     ELEQKERERTGIKSLKVGYNQVFGYYIEVTNSNLELVPPDYIRKQTLSGGERFINQTL
                     KEYEDLILNAHEKSIALEYQLFLDIRNTVLDSIDSLQTTAMALAELDALLSLAEAAVR
                     YHYVQPEMNQTGQILILDGRHPVVEKMLPNSGFVPNDTRLDEDENRTQIITGPNMAGK
                     STYMRQVALIVLMAHIGSFVPAKQAEIAVVDRIFTRVGASDDLATGQSTFMVEMNEVA
                     YILNHATQHSLVILDEIGRGTSTFDGLSIAWAVVEFINQRARIGCKTLVATHYHELTD
                     LENRLEGIKNYHVLVQRDGESIDFLRKIVPGKADESYGIEVARLAGLPAEITDRARKI
                     LKNLERGESCEFGMVVPEPRQTETESQVQLSLFPMEKELILEELKGIDLVATTPLQAL
                     NLLYQWQQKIRRNS"
     gene            5631..7406
                     /gene="mutL"
                     /locus_tag="VF531_01425"
     CDS             5631..7406
                     /gene="mutL"
                     /locus_tag="VF531_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX7712655.1"
                     /translation="MSSNKVHRLEEATIAQIAAGEIIERPASVVKELVENSIDAGATR
                     IEVEIRNGGRQLIKVTDNGCGMEREDAVLAIERHTTSKIEQPDDLLRIKTLGFRGEAL
                     PSIAAVSNFELITKTEGNEIGSLVEVKGGLLQKVKDVGAPTGTTIKAQDLFFNTPARL
                     KYLKTIPTEAGYIAEIISRLALGYPEISFRLNHHEYEVLFTPGNNDPYEAIVAVFGKD
                     SAKEMIPVSYEESGFKITGFIGKPTVAKTNRGNEIFFINRRYFHNRVLSAAVEKAYHT
                     LLPIARYPFVILFLDIDPQLVDINVHPSKMEVRFTNESELFKIFYHAVNKLLKDSCLL
                     SEWIVPDEEFNLKNITTTENQTPLENLSLYQESANQMPKAQAETATVAEPEAVTGYPV
                     PETVKNEVTEFYVFPKAIRDTFIVAQDEKGLLIIDQHAAHERILYERYLYKTREFLGS
                     QALLIPETVNLNHVQYKVVSERLTLFADLGFEMEVFGGQSLILRSVPLTLVNFDYHQI
                     ILDLLEDYLHFETFKNPAEIKEAFIMTMACRTAIKAGDTLNHLEMEGLVKNLFRSENP
                     YTCPHGRPTVFRMSYEELAKKFLRR"
     gene            7403..8362
                     /gene="miaA"
                     /locus_tag="VF531_01430"
     CDS             7403..8362
                     /gene="miaA"
                     /locus_tag="VF531_01430"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7712656.1"
                     /translation="MSKPPLVVIAGPTAVGKTALAIALAQKLNAEIISADSMQVYRYM
                     DIGTAKPSPAERQGIKHHLIDLVTPDCDFSVSDYQNYFHPALASILAAGKLPIFSGGT
                     GLYIRACLESFLTDNPAGADPEFRRVLHERAERDGSAALHQELQRIDPSAAGRIHPND
                     LRRVIRALEVYHSTGRPFSELQSRQPGETKYQVIYIFLDRDRTELYQRIDERVEQMFK
                     QGLVAEVRMLMDRGYGPDLKPMQSLGYRQICSFLHGSCTLDQAIAEMKSRTRNYAKRQ
                     LTWFRREPVDFWVNLSEKQSNNGFPREIFLEILEYLEGRLERM"
     gene            8403..8648
                     /gene="hfq"
                     /locus_tag="VF531_01435"
     CDS             8403..8648
                     /gene="hfq"
                     /locus_tag="VF531_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX7712657.1"
                     /translation="MNKPAINLQDAFLNQVRKEGIAVTVFLINGFQLRGLVKGFDNFT
                     IILDMDGKQEMVYKHAVSTIIPARPLPDFSAVEKKEE"
     gene            8697..9530
                     /gene="dapF"
                     /locus_tag="VF531_01440"
     CDS             8697..9530
                     /gene="dapF"
                     /locus_tag="VF531_01440"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7712658.1"
                     /translation="MKFTKMQGLGNDYIYIDLFTEKVTEPEELSRRMSDRHFGIGGDG
                     IVLMMPSNCASARMRIFNADGSEAEMCGNAIRCVGKYLYQHQRVHSQELTIETKSGIK
                     RLFLETSGDQVVSVRVDMGEPILERESIPVIGDSRHIMNEPITVNGETYRYTAVSMGN
                     PHCVIFVPEITESMVTIDGPALEAHPNFPRKINVEFIKVLDKNHLEMRVWERGSGETM
                     ACGTGASASLVAAVLNGLVQRKVRVQLRGGELEIEWDESSNHVFMTGPAVEVFGGEWP
                     E"
     gene            9553..10458
                     /locus_tag="VF531_01445"
     CDS             9553..10458
                     /locus_tag="VF531_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX7712659.1"
                     /translation="MTPRSGNSRLRIATLGPQGTYSERAARYFVKRLGMEPDRVGFFF
                     TTVLNSLRMVQNRQADYAVIPAENMIDGLIGSTFDALIEFQDFVKVCDEIHLPINHIL
                     AAPSNVKWDGIARIYSHPSALNQCQNRLAELFPDVELIAVASTAAAAELAAKDSTATS
                     AAICCDDTAQINRLAVYATDIQDYTVNETRFIVCGLTDAEPTGNDRTLLAVRYGANQP
                     GQLYQTAKHFAEAEIDLAFIQSRPYKVRPQEYVLIYELIGHKSDPRVELALKNVEFQV
                     RASNGWKKILGSYPKRERETTNDHT"
     gene            10445..>11494
                     /locus_tag="VF531_01450"
     CDS             10445..>11494
                     /locus_tag="VF531_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HEX7712660.1"
                     /translation="MIIPEALLERIERIEQELEPRFHDIARIAFQNQNKVLNAFQNAR
                     IGSHHFQTTSGYGYHDPGREALEQVFATVFKAEAALVRQQLVSGTHAIACAILGNLGP
                     GDELLIASVPPYETLFGVLGIPEDCVSAVSTSSVVSTTGAFTVRIVPLSSDCSLDLSA
                     ICRAITSRTRLVAFQRSCGYSERHSFSIAELQQAFQAVKTNHPELSIFVDNCYGEFVA
                     GEEPLEVGADLLAGSLIKNPGGCLVPSGGYIVGRTDLVEHAAERLYAPKIGGAIGPSL
                     INPQLFFQGFFEAPHRVGEMLKGALLTAALFEAAGFRVAPQVYEPRGDVIQKIYFKSA
                     PDLIRFCRAIQETSPI"
BASE COUNT         2808 a         2769 c         3024 g         2893 t
ORIGIN      
        1 cggcatcatc cggggcgggc gattgtctgg aatgtctatt tattgggggt gggagctttc
       61 tttcttatcc ccttgggttc aattttagcc cgttcctttc atgaactggg tttacccgac
      121 actcaactca cttttaccaa ctaccggctc cttttcgaca gtggatttca atttgcagta
      181 ggggcttctt tcggcaaggt actggctacc agcttgggtc tggctttggt tgttgccgtt
      241 ttgaccgtag gctgcggtta tttttttgcc aggagtcaaa gataccgacc ttgggggagt
      301 gtggacttcc ggttccaatt gccgcttggc atctcatttc tgacctttac caccgggctg
      361 gccgtcctgg ccgggaacta cctgccccca ggaatactaa tcgtttgggc tcagatcttt
      421 ttgacctttc cacttatcta ttccatgctt cgtaacgcct gccgcgaggt ggatcccgca
      481 ctcacagagg cagcggcgtc cctgggagcg actcggtttg tccggttgct cacggtggag
      541 ctgcccttgc tgaagaaacc tttggttact gctttcgcct atgccatggc cctatctctc
      601 ggagacttgg ctgctgtact gtcgctgggg aatggcgaag tggttactct ttcggtggct
      661 atctaccggt taatcgggca ttaccgtttt gcccaggcca atgccttggg taccatcttt
      721 atcgaattgg cttttggact gtttttggta atggaaggtt atgggagcca tttccggaat
      781 gtctggttga aaggagacgg gtacgatgac tgagccttta ttggatctgg cagtcaccaa
      841 agcctatccg gattttcagg tccaggtccg gttgaatgtg gctgcgggtg aatttttggc
      901 actgcttggc ccaagcggct gtggcaagac cactttgttg cgaattattg ccggtctgga
      961 acggcccgat aacggatggg ttcgattggc cggaagggat attacttttg ttgaaccggc
     1021 ggcccgaaga atcggtatgg tttttcaaga ttatgctctc tttcctcatc tgacagtagc
     1081 cggaaatatc gaatatggtt taaaggtgaa gaagatggcc gcggccgatc ggtcccggcg
     1141 ggttggtgag ttgctggaac tctttgaact gaatcatcta ggcaatcgct cggtcaatca
     1201 actctccggg ggtgaacgtc agcgggtggc cttggcccgg gcactggcac cccagccgtt
     1261 aatcctgctg cttgatgaac cgttcgccgc cttggattat ggtttacgcc gccggctccg
     1321 gaatgagctt cgggagctac aattgcgcct cgggtttacc gttgttttcg tcacccatca
     1381 acaggaagag gccttggccg tcgcggaccg gatcggcatc atgcaggcgg gaatggtttt
     1441 gcagacgggt cccggtaatg agatttatga acatccggcc aaccgttatg tagctgagct
     1501 cctgggagat gccaatctta ttccctgccg tatttccggg gaggacggcc aatacatcct
     1561 gcatgacggt aacgaagtgg ctctaccgtt tcaagaaaaa agcggtgcaa accaagttac
     1621 tccgcactcg gctaaaaccg gttacctcat gatccggcct gaggatctga ttttggatcc
     1681 gggcctggaa tatccggctt ttactgggac agtgaagagc ttggaatatc tgggctccac
     1741 ctatcaatta actgttgttt gtagcggggg acagatgtta caggcatttc tctccaaaca
     1801 ggaaggctgc cgtatccggc gtggccagat tttgcgtttg ggtttttcac ctcaggcggc
     1861 gcgttttctt tcggatattt cgtgatcttt tattacgtga tcttcgtgtg atggtggtta
     1921 tagtatttac ctggttggtt acctatacct attcaagaga tgcgtaaacg gcgggaggtt
     1981 tttggaagta ctgcgcgaat atttactctg aaccaagagt aatttggatt tatttaaccg
     2041 gcggaatgac ccgggggtgt cattgatgag acaaactgga cgcgggccca atcccgatct
     2101 ccaaaagatt ctttcgatct gggcagctgt cttgctcata attttagtaa cagcgtgttt
     2161 ctcgggaatt caggtatatt attggcaacg ttcggcggca gccaaagaac gggcaaacct
     2221 gatccggcaa aatcaacgcc ttgccgcaga ggtggcgcgt ctcaaggcgg ccttgaacca
     2281 tagtggacag gggagcaacc ccggggttca accaagggcg atttccgtga aacatcctgt
     2341 cgcccaaggc tcttccggta agagtccaac cattccatct aatttggcag ccagggagcg
     2401 aatggtactg gaagccctgc gtcgtcagga tatgaccgct ctcgcgcgat tggtcgatcc
     2461 cgaaaaggga ttacgttttt ccccgtattc ctatgtcaac attcagcaag acttggtttt
     2521 cgatgtagat caactcccgg gtcttttacg cgcgaagaca aactatcttt ggggatatta
     2581 tgacgggacc ggtctgccga tccggctgaa gtcggcggaa tattttaaat cattcgttta
     2641 cgacagggat tatttattgg acggcgcgga accgtcaagc ggcgcggtct taaaaaatag
     2701 cgtcactgct ggaaatgtat ttgaggttta tcccaaggcg gtcgtggtgg aataccgtga
     2761 tgccgaatcg aaaacctcca tgaactggca ttatttgaaa ctggtctttg aagcccggga
     2821 taaaacttgg tttctggtgg gactggtcca tgatcaatgg accatctgac tctggaaatt
     2881 atcatgtttt ttccatccag taaaaaggat aaaccgcgtt attgtcgaag attatcgggg
     2941 aaacccgatt tttttatgct ttgaggtgcg ggaatggtaa aggaaacccc ttgggtcaaa
     3001 cagtatctgg cgattaagaa ggattatccc gattgtctcc tcttttttcg gctgggagat
     3061 ttttacgaaa tgttctttga agatgccgtt atggcctcaa gggatcttga gcttgtcttg
     3121 accggcaaga attcaggaac agagcgaatc cccatgtgcg ggattccata tcacgccgta
     3181 tcgggctatc tggccaaact tctcgcgaaa ggtcacaagg tggccatctg cgaacagatg
     3241 gaagacccca agcttgccaa gggcttggta gagcgggctg taatccgggt ggtaaccccg
     3301 ggaaccgtga ttgaggatca gcttctcaag gaaaaggtta acaactattt ggccggagtg
     3361 gcgctggacg gccagttttg gggattggcc tatgtggatc tctccaccgg attatttcaa
     3421 actacccagt ttcccaaggc tcaaatccag atcctcctaa atgaactgac tcgcatccaa
     3481 ccggccgagc tttacctctc agaaaccgaa ctgcaaaccc ttaaccaacg gccggaatta
     3541 gccgatactt taattaccac cggtccctct gaccgatttc aaaccgaccg ggcttaccgg
     3601 tgcctaactg aacattttca gactcaaaat ttacgatctt ttggttgcga agaccggccc
     3661 gccgcgatta aggctgccgg tgcggtaatg gcctaccttt tggagactca aaaaacttcc
     3721 ctggaacata tccggaagat cacctcctat gaagtaggcg cctatatgac acttgatccg
     3781 gccacccgtc gcaacctgga actggtgaaa accatccgca gtaacgaagt gaacggtagc
     3841 ctgctctggg tgctcgatct gaccgttacc tctatgggcg gcagactgct ccggtcctgg
     3901 atcgaacagc cgttagccgg catcgaagcg atcaacgaac gccagggcgg cgtggctgaa
     3961 ttaacccacc gtttggaatt gcgggaatca ttacgggaag ccttgagccg gacctatgat
     4021 ttacagcgga ttctcagtaa actggcgaca aattcggcca atgcccgtga tctcttggcg
     4081 ctaaaaaata ctttagccga aattcccgaa atccgtgagt ttttaagcgg ggtaaccgcc
     4141 ccaaaactct tgatgctttt aaatgcgttg gatcctctgg cggatattac ccggctttta
     4201 caggaaagcc tcctcccgga tccgcccctt accgtaaaag aaggcggaat gatccggtcc
     4261 ggattcaacc cggaacttgc caaactgctc gaagcaggaa ccgaggggcg gcaatgggtg
     4321 gcggaacttg aacagaagga gcgggaacgt accgggatca aatcactcaa ggtcggttat
     4381 aatcaagtct tcggatatta tatcgaggtc accaatagca atctggaact ggttccaccg
     4441 gattatatcc gtaaacaaac gctttccggc ggagagcgtt tcattaacca gaccctaaag
     4501 gaatatgagg acctaatcct taacgcccat gagaaaagca tcgctctgga atatcaatta
     4561 tttttggata tccgaaatac ggtgctcgat tccattgata gcttgcagac taccgctatg
     4621 gctctggcgg agcttgatgc gttactctca ttggctgaag cggcagtgcg ttatcattat
     4681 gtccagccgg aaatgaacca gaccggacag atactgatcc tggacgggcg ccatccggta
     4741 gtggaaaaga tgctccctaa cagcggtttt gttcccaatg atacccgtct ggacgaggat
     4801 gaaaaccgga cccagatcat taccggaccg aatatggccg gaaaatccac atatatgcgc
     4861 caagtggcgc tgattgttct tatggcccat atcgggagtt ttgttccggc aaagcaagcg
     4921 gagatcgcgg tggtggatcg gatcttcacc cgagtcgggg cctccgacga cttggccacc
     4981 ggtcaaagca cctttatggt ggagatgaat gaggtcgcat atatcctgaa tcatgccact
     5041 cagcattctc tggtgatctt ggatgagatc ggccgtggca ccagtacttt cgacgggttg
     5101 agcattgcct gggcggtcgt ggagtttatc aaccaacggg cgcgaatcgg ctgtaaaacc
     5161 ctggtcgcga cccattatca cgagcttacc gatctggaga accggctgga gggcataaaa
     5221 aattatcatg tcttggtgca acgggatgga gagtccatcg actttttgcg gaagatcgta
     5281 ccgggtaaag ccgacgaaag ctatgggatc gaggtggccc gtttggcagg acttccagcg
     5341 gagattaccg atcgagcccg aaagattttg aaaaacctgg aacgcggtga aagttgtgaa
     5401 tttggaatgg tcgtgcccga acccagacag acggagactg aatcccaggt acaactctcc
     5461 ctgttcccaa tggagaaaga gttgattctg gaagagttga agggaattga tctggtagca
     5521 accacacccc tccaagcctt gaatctgctc taccagtggc agcaaaaaat acgccggaat
     5581 tcctgaaggc tctttaatat taacatccga taccaagtgg agggtctgaa atgagcagca
     5641 ataaagtgca tcgtttagaa gaagctacca ttgcccagat cgctgccgga gagattattg
     5701 aacggccggc atcggtcgtc aaggagttgg ttgagaactc gatcgacgcc ggcgccaccc
     5761 ggattgaggt ggagatacgc aacggaggcc ggcaattgat taaggtgacc gataacggtt
     5821 gcggcatgga gcgggaggat gcggtactgg ccattgaacg tcataccacc agtaagattg
     5881 aacaaccgga tgacctgtta cggataaaga ccctaggttt tcgaggggag gcgttgccct
     5941 caatcgccgc agtttccaat ttcgaattaa taaccaagac cgaaggaaat gagatcggat
     6001 cactggtgga ggtcaaggga gggctgctac aaaaggtaaa ggatgtggga gcgcctaccg
     6061 gaactaccat caaggctcag gatcttttct ttaatacacc ggcgagattg aagtatttaa
     6121 agaccatccc caccgaggcg gggtatatcg ccgagatcat cagccgtttg gccctgggat
     6181 atccggagat cagtttccga ctgaaccatc atgagtatga ggtacttttt acacccggca
     6241 ataatgatcc ctatgaggct attgtggctg ttttcggaaa agacagcgcc aaagagatga
     6301 ttcccgttag ttatgaggag tctggtttca agattaccgg tttcatcggg aaacccaccg
     6361 ttgccaagac caaccggggc aatgagatct tcttcattaa ccgccgttat tttcataacc
     6421 gtgttttaag cgctgcagta gaaaaggctt accatacctt gctgccgatt gcccgttatc
     6481 cttttgtcat cctctttttg gatattgatc ctcaattagt cgatatcaac gttcacccct
     6541 ccaaaatgga ggtgcgtttc actaacgagt ccgaactttt taaaatattc tatcatgcgg
     6601 tcaataaatt attgaaagat agctgcctcc tgtcggaatg gatcgttccc gatgaggagt
     6661 tcaacttaaa aaatattaca accactgaaa atcaaacgcc cctggaaaat ctctccttat
     6721 accaagaatc cgccaaccag atgccgaagg ctcaagccga gacggcaact gttgccgaac
     6781 cggaagctgt gacgggatat ccggttccgg agacggtaaa gaacgaggtt acggaatttt
     6841 atgtatttcc taaagcgatt cgcgacacct tcatcgtggc tcaagacgag aaaggacttt
     6901 taatcatcga tcagcacgcc gcccatgaac ggattctcta tgagcggtat ttatataaga
     6961 ccagggaatt tttgggatct caggcattat taatcccgga aacggttaat ctaaatcatg
     7021 tccagtataa agtggtatcg gaacggctga cgctattcgc cgatctgggt ttcgagatgg
     7081 aggttttcgg aggtcagtcc ttgattttac gtagtgtacc gctgacactg gtgaactttg
     7141 attaccatca aattattctt gatcttttgg aagattattt acattttgaa acctttaaaa
     7201 accccgccga gatcaaagaa gccttcatca tgaccatggc ctgccgaact gcgattaaag
     7261 ccggggatac cctgaaccat ctggagatgg aaggtttggt gaaaaactta ttccgttcgg
     7321 aaaatcccta tacctgccca catggccggc ctaccgtctt ccgtatgagt tatgaggaat
     7381 tggccaaaaa gtttttgcgt cgatgagcaa accgccgtta gtcgtgattg ccggtccgac
     7441 ggcggtagga aaaaccgcct tggccatcgc cttggcacaa aagctgaacg ccgaaatcat
     7501 ctccgcagat tcgatgcagg tttatcgtta tatggatatc ggaactgcga agcccagtcc
     7561 ggctgaacgt cagggaatca aacatcacct cattgacttg gtaactccgg attgcgattt
     7621 ttccgtatcc gattatcaaa attatttcca tccggcgctt gcctccatat tagccgctgg
     7681 taaactgcct atcttttccg gcggtaccgg tctttatatt cgggcttgtc tggaatcttt
     7741 tttaaccgac aatccggccg gcgccgatcc cgaattccgg cgcgttcttc acgaacgggc
     7801 cgaaagggat ggctctgcgg cactccatca agaattgcaa cggattgatc ccagcgccgc
     7861 cgggcggatc caccctaatg atctgcggcg ggtcatccgg gcgctggaag tttatcatag
     7921 caccggccgg cctttttcgg aattacaatc gcgccagccg ggagaaacaa aataccaggt
     7981 aatctatatc tttttggatc gggaccggac cgaattgtac cagcggattg acgaacgggt
     8041 tgagcagatg ttcaaacaag gtttggtagc agaggtccgg atgttaatgg atcgaggtta
     8101 cggaccggac ttaaaaccca tgcaaagttt aggctaccgc cagatctgca gtttcctcca
     8161 cggatcctgc acgctggatc aagcaatagc cgagatgaag tccaggacac gaaattacgc
     8221 caaacgtcaa ttaacatggt ttcgccggga accggtggat ttttgggtga atctgtcgga
     8281 aaaacagagt aataacggct ttcctcggga aatttttctc gaaatcttgg aatatcttga
     8341 aggtagattg gagcgaatgt agaataacta atgttaaacc agtttcattg gagggggatt
     8401 tgatgaacaa acctgcgatc aacctgcaag acgccttttt aaaccaggta cgtaaagaag
     8461 gaatagcagt cactgttttt ctgataaacg gttttcaact ccgaggattg gtaaagggtt
     8521 ttgataactt caccattatt ctcgatatgg acggaaaaca agagatggtt tataaacacg
     8581 cggtatccac cattattccg gcccgtcctt taccagattt ctcggcagtg gaaaagaaag
     8641 aagaataaag acccgggagg gtctttttca atcattaaac tacggtggag tatgcgatga
     8701 aattcaccaa aatgcaaggc cttggaaacg actatatcta tatcgacctc ttcacggaga
     8761 aggtcaccga acccgaagag ttgtctcgca ggatgagcga ccgtcatttc gggatcggcg
     8821 gtgacggcat tgtgctcatg atgccgtcga attgcgcctc ggcccggatg agaatcttca
     8881 acgccgatgg cagtgaggct gagatgtgcg gtaatgccat tcgttgcgtc ggaaaatacc
     8941 tctatcagca tcaacgggtc cattcccaag aattgacgat tgaaaccaaa tcaggaataa
     9001 aacgtttgtt cttagaaacc tccggggatc aggtcgtttc ggtccgggtg gatatggggg
     9061 agcccattct tgagcgcgag tccatcccgg tcattggaga ttcacgtcat attatgaatg
     9121 aaccgatcac agtcaatggc gagacttaca ggtataccgc agtttctatg ggtaacccgc
     9181 attgcgtcat ttttgttccg gagatcaccg agtcaatggt gacaattgac ggacccgcac
     9241 ttgaggccca tcccaatttt ccccgcaaga tcaatgtgga attcatcaag gtccttgaca
     9301 aaaatcatct tgaaatgcgg gtctgggaga ggggtagcgg cgagacaatg gcttgtggaa
     9361 ccggtgccag tgcctccctg gtcgctgctg ttttgaatgg tttggttcag cggaaggtac
     9421 gggtccaatt acgtggcggt gaattagaga ttgaatggga tgaatccagt aatcatgtat
     9481 tcatgaccgg accggcggtt gaggtcttcg ggggggaatg gccggaatga gagagtgcgt
     9541 ccggaggatt cgatgacccc ccggtcggga aattcccggt tacggatcgc gaccttgggc
     9601 ccgcaaggga cctattcgga gcgggctgcc cgttattttg tgaagcggct ggggatggag
     9661 ccggaccggg tggggttttt ctttactacg gtcttaaact cattgcgaat ggttcaaaat
     9721 cgtcaagccg attatgcggt cattcctgcc gaaaacatga ttgacggcct aattggctca
     9781 actttcgacg ctttaatcga gtttcaggat tttgtcaaag tttgtgatga gattcatctg
     9841 ccgatcaatc acattttggc ggccccttcc aatgtgaagt gggatggcat cgccagaatt
     9901 tattcccatc catcggcatt aaaccagtgt cagaatcgtt tggcggaact ctttcccgat
     9961 gtggaactga tagcggtggc atccaccgct gcagccgccg aattggccgc caaagacagc
    10021 accgcaacca gtgctgcgat ctgctgtgat gatacagctc aaatcaaccg gcttgcggtt
    10081 tatgcgacag atatccagga ttacaccgtt aacgagaccc gttttatcgt ctgcggcctg
    10141 actgacgccg aaccaaccgg gaacgaccgg accttactgg cagttcgata cggtgcaaac
    10201 cagcccggcc aattgtatca gaccgcaaaa cattttgcgg aggcagagat cgatctggca
    10261 tttatccaat ccaggcccta taaagtgaga ccgcaggagt acgttctaat ttacgaatta
    10321 atcgggcata aatccgatcc ccgggtcgag ctggcattaa aaaatgtaga gtttcaggtc
    10381 cgggcttcaa acggctggaa gaaaatttta gggagctacc ccaagagaga acgggaaacc
    10441 actaatgatc atacctgaag cgcttctgga acggattgaa cgaatcgaac aggaacttga
    10501 gcctaggttc catgatatag cgcgaatcgc ttttcaaaat cagaataaag tattgaacgc
    10561 ctttcaaaat gcccggatcg gcagccatca ttttcaaacc accagtggtt acggatatca
    10621 cgatcccggc cgggaagccc tggagcaagt cttcgccacc gtctttaaag ccgaggctgc
    10681 cttggtacgt cagcaactgg tctccggaac acatgccatc gcctgcgcca tcttgggcaa
    10741 ccttggcccc ggcgacgaac tgctaatagc ctccgtgcca ccttatgaaa cccttttcgg
    10801 cgtgttagga atccctgagg attgtgttag cgctgtcagc accagcagtg tcgtaagtac
    10861 caccggcgct tttacggtac ggattgttcc tttatcttcc gattgctcgc tggatctgtc
    10921 tgccatttgc cgcgccataa cttcacggac ccggctggta gcatttcagc gttcttgcgg
    10981 atactctgaa cgtcattcct tctccattgc cgaacttcag caggcttttc aagcggtgaa
    11041 aacgaaccat cccgagctaa gtatctttgt cgacaattgt tatggtgagt ttgtcgccgg
    11101 ggaggaaccg cttgaggtcg gtgcggattt actggccgga tctttgatca aaaaccccgg
    11161 ggggtgtctg gtacccagtg gcggctatat cgtaggccgg actgatctgg tagaacatgc
    11221 cgcggaaagg ctttacgccc caaagatcgg cggagcgatc ggcccttcac tcatcaatcc
    11281 acagctattc tttcaaggtt tctttgaagc gccccatcgg gtgggagaga tgttgaaggg
    11341 agcattatta acagctgcgc tattcgaagc ggctggattc agggtggcac cgcaagtcta
    11401 cgaacctcgc ggtgatgtga ttcaaaaaat ttacttcaag tcggccccgg atttaatccg
    11461 tttttgccgg gcgattcagg aaacttcacc gatt
//
LOCUS       DASVMB010000043        10084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_519810,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000043 DASVMB010000000
VERSION     DASVMB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519810"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VF531_01455"
     CDS             complement(<1..1068)
                     /locus_tag="VF531_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX7712661.1"
                     /translation="MLIKRVDTYAIDVPAPHKGGQIWVFVKLTTDNGIEGWGECNAIF
                     SKEKTVVQLVNELAEKAIIGKMNPFNIEKLWMSLYEFDGCHFSRHPAVVENQAIAAIE
                     MACWDIVGKSLNQPIYNLLGGVYHEKLRSYTYMNYRDWSPGKAADIAAREAAQLVKEG
                     FTAIKLDPIIPLCPQPRELTMKDLQYAEDVIRSVREAVGNNADILIGTHGQFTTSMAI
                     RFAKRMEKYDPFWFEEPVPMENVDEMAQVARATTIPVATGERLATKWEYLPLFEKHAA
                     RIIQINVGLNGILESKKIAGMAESHFAQISPWMYCGPIAGAANIQLDVCSPNFVMQEG
                     IEHWDGFHAEILKEPIKWEKGY"
     gene            complement(1244..2548)
                     /locus_tag="VF531_01460"
     CDS             complement(1244..2548)
                     /locus_tag="VF531_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7712662.1"
                     /translation="MKSGKRTRFFLTILSFAVLVACLGLAPTTMAAAPQKTISFWCQK
                     WWPVGYNEGMVAAAKDFEKETGVKVDLFMAGDVEVRTKVQVAIETKTLPDVGFIMSAE
                     PTMYHELGVLAPVTDVVNHLKKKYGGLYGDITCGKFGSQYYAVPYYSIPDVLHVRKDR
                     FDQAGLALPQTWDEVMAAAKKTTDAAKNIYGFGEGVGAGNVDHGKLLRTLMYSFGGGE
                     FAKNGKTITIGSPQSLKAFAWVKEGWKAGIFPPDGTQWDSSGNNKSWLSGQATMIWNA
                     ASTIVSTQSQAPDIYKNMAMVKVPAGPNGVRAQFSDVQNLVVFKTSKNVALAKKFIEF
                     LFQPKYYSKWVMPSGQPTFQSFVSDPMWNDPLLKPVIETSQYIKNMGYPGPLTPWAAE
                     MFQRNILEQMSSEMVLKNLTPEEAAKEYIPKIKEIMNRWQKK"
     gene            complement(2684..3544)
                     /locus_tag="VF531_01465"
     CDS             complement(2684..3544)
                     /locus_tag="VF531_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712663.1"
                     /translation="MKKETRNGYLLVLPLICVFLLFTAYPLFSGIAMSFYNKRIGMPG
                     MFVGFDNYLTILKDSAFISTTVNSFIYTLSAEFYKLILGLTIALVLNEDFKGRTFFRG
                     LFLLPWILPTFVAAHTWRWIFDYNGILNQVLKSLGLIQQQIAWLGDPRTAMACIIFVN
                     VWKGFPFFGLSFLAALQTIGRDIYEAADIDGASWFQQFRYITLPNIKSIIFLVLTLST
                     IWTFNDYVLVHILTDGGPVQMTNILPIYIFKMAFVSLLPSAAITAAMFLVPLLGVLIF
                     ALTRALKTQE"
     gene            complement(3603..4433)
                     /locus_tag="VF531_01470"
     CDS             complement(3603..4433)
                     /locus_tag="VF531_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712664.1"
                     /translation="MQLKATKKRNIILIVIIALFTVFTMFPIYWMVCTSFSSIADLYH
                     KQSLLPGKLTLAGYIGLFRETNFRHYYLNSLIVSGLSTLIALPISFLGAFSLIRLKWR
                     GQNFLKSIVLWTYVLPEVILVIPLYVILNSIHLLNSTIGLVLTFLIGSTPFCTWLLMG
                     YFQGIPKELEDSAKIDGCNILSMIMRIILPVSAPGVVTAGIYAFNLSWALYLYPVVFI
                     TQESKQLLAPGIVMLQIGDMFPWDQIMAAGVLACLIPGIVYTIGQKYVVQGLTLGAVK
                     "
     gene            complement(4450..5118)
                     /locus_tag="VF531_01475"
     CDS             complement(4450..5118)
                     /locus_tag="VF531_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7712665.1"
                     /translation="MASGDFVDENDVNIRSSMRDKIAERLREMILTQKLPPGSRISEY
                     ELSKLFKVSKTPVREAINIVKGEGLIAGESWQGIYVFKPTRKDVDDIFSIRYILEIEA
                     VNQVMDHLTEVHRKKFEEFIEVFQKVTSSFEKDAYVKLDTEFHTYIVKATGNDWLYKI
                     IGKLSQVTTMIRALTLKSTDIEFAKNHIIICEAILAKDKQAAQELMRQHIQSAWNDTL
                     KIIS"
     gene            5476..6477
                     /gene="yiaK"
                     /locus_tag="VF531_01480"
     CDS             5476..6477
                     /gene="yiaK"
                     /locus_tag="VF531_01480"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HEX7712666.1"
                     /translation="MIRVPFERMYGEFYRVLKKIGFDENKAALCGRLFAEASRDGVYT
                     HGLNRFPGFVAEVKAGNVMINGFPEKVADLAGLEIWDGKLGPGNVNAHFSMNHAMELA
                     RQNGIGCVALRNTNHWMRGGAYGWQAAEAGYVGICWTNTSPLVPPWGATEPKVGNNPL
                     ILAVPRAAGPIVLDMSMTMFSFGKIETYIRKGELLPVDGGFTQNGEVTRNPKEILESR
                     RPMPIGLWKGTGLALLLDLIGLFLSGGNSTYQLGKQNQTGGFSQIFLAFNISGLPHRD
                     SLPQMVEEIIEDFHRAQPEHESEKVLYPGERVIQTRLENPKKGIPVEELFWNQVINM"
     gene            6512..7504
                     /locus_tag="VF531_01485"
     CDS             6512..7504
                     /locus_tag="VF531_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7712667.1"
                     /translation="MMKTIKLGVLGVSKHFITRVLPGVLKSGCIEVYAIASRDSAKAR
                     QASEKFGIPLYYASYDELVKDKSLEIIYIPLPNHLHTEWIKRSADYGKHIICKKPLAL
                     NAHEAREAIDYATAKGVKIMEAFMYRFHPQWQRALEIVNSGEIGTLTTIHTIFGYFNA
                     DPANIRNIKSVGGGAIYDIGCYAVSSIRFLLQAEPRQVLCMLDRDPGFQTDIRANAIL
                     DFGEIRTLFTVGTQTFPAQSVEVYGTKGIMTIEIPFNPYPDSPAKIRIQSKGQTRILE
                     LGPADQYQLEFDSFADAILNNRPVPTPPEDAIHNQRVLDALFQSAQTGQWQKVD"
     gene            7730..8581
                     /locus_tag="VF531_01490"
     CDS             7730..8581
                     /locus_tag="VF531_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7712668.1"
                     /translation="MPESPDSKDILTLEEASLLFQVSTKTFLKLLREEEIPARKVGRE
                     WRFSRTALLEWMASGSSRQYTAMEEATHTYFTQVAPVYDEERKSCYAGALRELLISRF
                     PPHPTYQVADIGTGTGYLAEALAKQAHEVMAIDASEAMLAVAKKNFSQTGLSNIQFII
                     GDAQDLPLPDASLDMTFANLLLHHLFEPEQAIKEMYRVLKPDCIAVISDVEQHPHTWV
                     QQEKSDIWLGFSPREILGWMQKAGFHESFVTGLGCNCRTSSRSGLTIEIPIFVAYGKK
                     TVDQKPV"
     gene            8616..9881
                     /gene="leuC"
                     /locus_tag="VF531_01495"
     CDS             8616..9881
                     /gene="leuC"
                     /locus_tag="VF531_01495"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7712669.1"
                     /translation="MGMTITQKILAAHAGKKTVTPGELINARVDLILGNDITAPVAIR
                     EFYKIGVDKVFDPDRIALVPDHFVPNKDIKSAEQARMMREFARKHRITNYFEVGSMGV
                     EHCLLPEQGLVLPGELIIGADSHTCTYGALGAFATGIGSTDMAAAMALGEAWFKVPES
                     IKFIYHGNLPKWIGGKDLILFTIGQIGVDGALYQAMEFGGEAIAALPLEGRLTMANMA
                     VEAGAKNGIFPVDDRTLEYLHSRATRSYSVFESDCDAVYAKVFEWDISGLEPQVALPH
                     LPEKTRPVSACRGINLDQVIIGSCTNGRILDLREAAAVLKGRHVNPNVRLIIIPGTPA
                     IWRQAMREGLFEVFMDAGAAISTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGDP
                     GSEVYLAGPAVAAASAITGAIAGPEEVGA"
     gene            9878..>10084
                     /gene="leuD"
                     /locus_tag="VF531_01500"
     CDS             9878..>10084
                     /gene="leuD"
                     /locus_tag="VF531_01500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823905.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7712670.1"
                     /translation="MSLFHGNAWTFGSDINTDLIIPARYLNTSDPRELALHCMEDADP
                     GFPAKVRPGDILVAGKNFGCGSSRE"
BASE COUNT         2765 a         2398 c         2268 g         2653 t
ORIGIN      
        1 gtaccctttc tcccacttaa tcggctcttt taagatttca gcatggaaac cgtcccaatg
       61 ctcgatacct tcctgcatta caaaattggg actgcataca tccaattgaa tattggctgc
      121 tccggcgatt ggcccacagt acatccacgg cgaaatctgt gcgaagtgcg actcggccat
      181 accggcaatt ttcttcgatt ccaggatacc gtttaatccg acattgattt gaataatacg
      241 ggcggcatgt ttttcaaaca agggcagata ctcccatttg gtagccagac gttccccggt
      301 tgccaccgga attgtcgtcg cccgtgccac ctgggccatt tcgtcgacat tctccattgg
      361 gaccggctct tcaaaccaaa atggatcata tttttccatc cttttggcga agcggattgc
      421 catactggtg gtaaactgcc cgtgtgttcc gattaaaatg tcggcattgt tccctaccgc
      481 ttctcttaca gaccgtatca catcttcagc atactgcaga tccttcatgg taagctctcg
      541 cggttgcggg cacaacggga tgatgggatc cagctttatt gctgtaaagc cctcctttac
      601 caattgggcc gcttccctgg ctgctatatc cgctgctttt ccgggtgacc agtccctata
      661 attcatatag gtataggagc gtaatttttc atgataaacg ccgcctagca gattatagat
      721 aggctgattc aatgatttac cgacaatatc ccagcaagcc atctcaattg cagcaatcgc
      781 ttgattttca acaactgcgg gatgccgcga aaaatgacac ccgtcgaatt catacaagga
      841 catccatagc ttttcaatat taaatggatt catcttgcca ataatggctt tttcagctaa
      901 ttcgttaacc agttgaacaa cggtcttttc tttggaaaaa atcgcgttac attcgcccca
      961 accttcgatt ccattgtctg tggtcagttt tacaaaaacc caaatctgcc ctcctttatg
     1021 aggagccggt acatcaattg cataagtatc aacgcgttta ataagcatca atcttacccc
     1081 ctaaatacgg attaataatc ttatgatgtc aaatttcagc caaaatccgc ctgatttcag
     1141 gtaccggagg tgaacttgga cttttagtgg accgggttgg gattgccaac ccggtccatg
     1201 gaaaacggtt ttcttatgga gtttttaaac cggtctcaaa agcttacttc ttttgccacc
     1261 tgttcatgat ttccttaatt ttaggaatgt actctttggc agcttcttca ggtgttaaat
     1321 tcttaagtac catttcggag gacatttgct ccaaaatatt tctctggaac atttcagcgg
     1381 cccaaggagt gagcggaccc ggataaccca tattcttaat atattgggaa gtctcgataa
     1441 ccggtttcag caaaggatca ttccacatcg gatccgaaac gaaagattgg aacgtcggtt
     1501 ggccgctggg cataacccat ttggaataat acttgggctg gaacaggaat tcaataaact
     1561 ttttagctaa agcgacattt ttggaagtct tgaatacaac caagttctga acatcggaaa
     1621 attgagccct tactccgttg ggaccagccg gaaccttgac catcgccata ttcttataaa
     1681 tgtcaggagc ttggctctga gtggaaacaa tcgtagatgc ggcattccaa atcatggttg
     1741 cctgaccgga taaccaggat ttgttatttc cgctggaatc ccattgggtc ccatccggcg
     1801 ggaagatacc ggccttccag ccttctttga cccaagcaaa ggctttgagg gattgaggtg
     1861 aaccaatcgt aatcgtctta ccatttttgg cgaattcacc gccgccaaag gagtacatca
     1921 gagttctcaa caatttgccg tgatctacgt tgccggcacc tactccttca ccgaaaccat
     1981 agatgttctt tgcagcatca gtggtttttt tcgcagcagc cataacttca tcccaagttt
     2041 gcggaagggc caggcctgct tggtcaaagc gatccttccg gacatgtaaa acatcgggaa
     2101 tgctgtaata gggcactgca taatattggc tgccaaattt tccgcaggta atgtcaccat
     2161 acaaaccgcc gtatttcttt ttcaaatggt tgactacatc ggtaaccggt gccaaaaccc
     2221 cgagttcatg gtacatcgtg ggttcagccg acataatgaa ccctacatcc ggcaatgtct
     2281 tggtctcaat tgccacttgg actttggtcc ggacttccac atctccggcc ataaagaggt
     2341 caacctttac tccggtttcc ttttcaaaat ccttggctgc ggcgaccatt ccttcattat
     2401 aacctaccgg ccaccacttc tggcaccaaa aagagattgt cttctgcggt gccgctgcca
     2461 tggtggtcgg ggccaaacct aagcaggcaa cgagtactgc gaatgataaa atcgttaaga
     2521 aaaacctcgt tcttttaccc gatttcattc aaattaccct cctctttgtt ggttgaaaat
     2581 ctaatcaatt tcgtagatct agaggaagac cttttcgcca ttcaacacct cctttcaaag
     2641 aatctctccc aggccggtga tgccaagaat tctgttggaa aagtcattct tgggttttta
     2701 atgctctggt caatgcgaag attagaactc ccaagagcgg aaccaggaac attgcagctg
     2761 ttatcgccgc actgggaaga agactgacaa acgccatctt aaaaatatag attggcaaaa
     2821 tattggtcat ctgtacagga ccgccgtccg ttaagatatg aaccaagaca tagtcgttga
     2881 aggtccagat agtggaaagg gtgagtacta aaaagatgat cgatttgatg ttgggtaaag
     2941 taatatatcg aaactgctga aaccaggagg caccgtcaat atctgccgct tcgtatatgt
     3001 ccctgccaat cgtttgcaat gccgcaagaa aactcaagcc gaagaaggga aagcccttcc
     3061 agacatttac aaagatgata caggccatgg cagtccgggg atcacccaac caggcaattt
     3121 gttgttgaat cagacctaaa cttttaagta cctgatttag aatcccatta tagtcaaaaa
     3181 tccatctcca agtatgagcc gccacaaatg taggaagaat ccagggaagt aaaaacaatc
     3241 cccgaaaaaa tgttctgcct ttaaaatcct cattaagcac caaggcgatg gttaacccca
     3301 agattaactt atagaactcg gccgataggg tgtatataaa actatttacg gtagtggaaa
     3361 taaaggctga atcctttagg atggtgagat agttgtcaaa gcccacaaac ataccaggca
     3421 taccaattct tttgttatag aaactcatgg caatacccga aaacaaagga tacgctgtaa
     3481 ataataagaa gacacaaatc aaagggagta ctaataaata cccgtttcta gtttccttct
     3541 tcatagatgt tcacgctccc ccatgaatta aaaaggttaa aaatcgtaat cgaacgactg
     3601 gtttatttaa cagctcctaa agttagtccc tgcactacat atttctgacc aatcgtatag
     3661 acgattcccg gaataaggca ggccaggaca ccggcagcca tgatttgatc ccagggaaac
     3721 atatcgccga tttgtaacat tacaattccc ggagccaata actgctttga ctcttgggtg
     3781 ataaacacta cgggatacaa atacaatgcc catgaaagat tgaaggcgta gattccggct
     3841 gtgaccactc cgggagcgga aaccggtaga ataatgcgca taatcattga taggatgttg
     3901 caaccgtcga tctttgccga atcctccaac tctttgggta tcccttgaaa gtaacccatg
     3961 agcaaccagg tgcagaatgg agtactgccg ataagaaatg tcagcaccag tccaatcgtc
     4021 gaatttagca gatgaatact attaagtatc acatacaacg gaatgactag aataacttca
     4081 ggtaagacat aggtccataa cacaattgac tttagaaagt tttgcccccg ccactttagg
     4141 cgtattaagc tgaatgcgcc caaaaaactg atgggtaacg caataagagt tgatagtccc
     4201 gatacaatga gactattgag gtaataatga cggaagttcg tttctctaaa aagtccgata
     4261 taaccggcaa gtgttaattt gcccggtaag agagattgtt tatgatacag gtctgcaatg
     4321 cttgaaaagg aagtgcaaac catccagtag ataggaaaca ttgtaaagac ggtaaatagg
     4381 gcaataatta cgattaagat aatgtttcgc ttttttgtgg ccttaagttg cattttttat
     4441 cttccttttt tacgagatga tttttaaagt atcattccaa gcgctttgaa tatgttgcct
     4501 catgagttcc tgcgcagctt gtttatcctt cgctaaaatg gcctcgcaga taatgatatg
     4561 gttttttgca aattcaatat ccgtgctttt taaagtcaat gctcggatca ttgtggttac
     4621 ttgacttaac ttaccgatta ttttataaag ccaatcatta ccagtggctt tgacgatata
     4681 tgtatgaaat tcggtgtcaa gttttacgta ggcatctttc tcaaaagaac tggtgacttt
     4741 ctgaaaaacc tcgataaatt cttcaaattt cttgcgatgc acttccgtta aatgatccat
     4801 aacctggtta actgcctcga tttccagaat atagcggatc gaaaaaatat catcgacatc
     4861 cttacgagtg ggtttaaaaa catagatccc ctgccaagac tctcccgcga tcaaaccttc
     4921 accttttacg atgttaattg cttcacgaac cggcgtcttg ctcactttga atagtttaga
     4981 gagttcatat tcggatattc tcgagccggg aggcaacttt tgggtaagga tcatctccct
     5041 taaccgctca gcaatcttat cacgcatgct tgaacgaata ttcacatcgt tttcgtcaac
     5101 aaaatctccg gaggccattc tgcatctccc ttccatttta tgtattataa tttatcttat
     5161 ataaagaata tattataata gatatatctg gcaaacctgg ttttctccga tattaggaaa
     5221 aaagcgttac tatcggtcat tttaccacgt ttttaggttt cagttacacg ttgtttataa
     5281 tatcgtcaaa tttggcatgt ggatccttga ccaggcttat aattccagta aaaatatttt
     5341 tccccctgaa taaatttcct ttagttagct actaattaca agaatgaatt catgattgtg
     5401 ataaaagctc agattcttct gaaacttttg gtatggggga gaatcacggg tgaaaataaa
     5461 cgagaggtga tggatatgat acgggtgcct tttgaacgaa tgtatgggga gttttatcgg
     5521 gtgttaaaga aaattggttt cgacgaaaac aaagcagcat tatgcggaag actttttgcc
     5581 gaggccagtc gagatggggt ctatactcac ggcctaaatc gtttccccgg gtttgtagca
     5641 gaagtaaagg caggtaacgt aatgatcaac ggtttccctg aaaaagtggc cgatttggct
     5701 ggccttgaga tatgggatgg aaagctggga ccaggtaacg taaacgccca tttctccatg
     5761 aatcatgcga tggaactggc aagacaaaac ggaatcggtt gcgttgctct acgaaatacc
     5821 aaccactgga tgcgaggcgg agcctatggg tggcaagccg ctgaagccgg atatgtcggt
     5881 atctgttgga ccaacacctc ccctctagtc ccgccgtggg gagctaccga gcctaaagtg
     5941 ggaaataatc cgctcattct cgccgtgcct cgtgccgccg gaccaattgt ccttgatatg
     6001 tccatgacca tgttttcatt cggcaaaatt gaaacctata tccgaaaagg agaattattg
     6061 cctgtagatg gtggttttac tcaaaacggg gaggtaaccc gaaacccgaa agagattctt
     6121 gagtccagga ggcccatgcc gataggatta tggaaaggta ccggtcttgc tctactattg
     6181 gatttaatcg ggttattttt atccggaggc aactccactt atcaattggg taagcagaac
     6241 caaaccggtg gtttttccca gatattcttg gcatttaata tttccggctt accccatcgg
     6301 gactccctcc ctcaaatggt cgaagagatc attgaagatt ttcaccgggc ccaaccggaa
     6361 catgaaagtg aaaaggttct ttatcctggc gaacgggtga tacaaacccg tcttgaaaat
     6421 ccgaaaaaag ggattccagt tgaagaatta ttttggaatc aggtaattaa catgtaagtt
     6481 atcaataaat ccattctaaa ctggggggtc gatgatgaaa actattaagt taggagttct
     6541 cggtgtttcg aaacatttta tcacgagggt tctgccgggg gtactaaagt caggctgtat
     6601 cgaagtatac gccattgcct cacgcgacag cgccaaagcc cggcaagctt cggaaaaatt
     6661 cggcattccg ttatactatg cctcttacga cgaattggta aaagacaaat ctctggagat
     6721 aatctacata ccgcttccca accatttaca taccgaatgg atcaaaagat cggccgatta
     6781 cggcaaacat attatctgta aaaagccatt agctttaaat gctcacgaag cccgggaagc
     6841 aatcgattat gctactgcca aaggagttaa gatcatggag gcatttatgt accgcttcca
     6901 cccccagtgg caacgtgctc tggagatcgt caactccgga gaaattggga cgcttaccac
     6961 tatccatacc attttcgggt acttcaacgc cgatccggca aatattcgca acattaaatc
     7021 cgtcggtggc ggggcgatct acgatattgg ctgttacgca gtgtcttcca tccgattttt
     7081 acttcaagcc gaaccccgac aagtactctg catgctcgat cgtgatcccg gttttcaaac
     7141 ggatattcgg gccaatgcca tcttggattt cggagaaata cggaccttat ttacagtcgg
     7201 tacccaaact tttcctgccc aaagcgtgga ggtatacggg accaaaggga taatgaccat
     7261 tgaaattcct tttaacccgt atcccgattc tcctgccaaa atccggattc agagcaaagg
     7321 tcaaacccgg attttggaac ttggaccggc cgatcaatac caactggaat ttgattcttt
     7381 cgcagacgcc atcttaaaca atcgtccggt tcccactcca ccggaggatg ccatccataa
     7441 ccaaagagta ttggatgcac tgtttcaatc cgcccaaacc ggtcagtggc aaaaagttga
     7501 ttgaaacaag ccacacaaat gattctcatt gaataccggt cttacttgag ccaagtaagg
     7561 accggttttt tattcccttc attaccacgg acctgaaaac ccaatcggga aaaatcacct
     7621 gcataggaat ataagagaac ttataagaat atgagagaat aaaacccttg acatttttcc
     7681 ctaaaaccct ttataatgat aaaaactgcc aaccaaagga tcgaatgcta tgcccgaatc
     7741 ccctgattcc aaggatattt tgaccctgga ggaagccagt ctgctttttc aggtaagtac
     7801 caagaccttt cttaaattat tgcgcgaaga ggagatccca gcccgtaaag tgggacggga
     7861 atggcgcttt tcccgcacag ccttgctgga atggatggcg agtggcagtt cacgacagta
     7921 tactgccatg gaagaagcta cccataccta ctttactcaa gtcgcgcctg tctatgatga
     7981 ggagcggaaa agttgttacg caggggcttt acgtgaacta ctcatctccc gtttcccgcc
     8041 ccaccccacg taccaggtgg cggatatcgg aaccggaacc ggttatctgg cggaagccct
     8101 cgccaaacaa gctcacgaag ttatggcaat tgatgcctcc gaagccatgt tggcagtggc
     8161 gaaaaaaaac tttagccaaa ccggattaag caatattcaa ttcatcattg gtgacgctca
     8221 ggatctaccg ctgcccgatg cctctctgga tatgaccttc gccaatctgt tactgcacca
     8281 tctattcgag ccggaacagg ccatcaagga aatgtatcga gtgcttaaac ccgattgtat
     8341 cgcggtcatc agcgatgtcg aacaacatcc ccacacctgg gtgcaacagg aaaagtccga
     8401 catctggctg ggatttagtc cccgggaaat tttgggatgg atgcagaaag ccggattcca
     8461 cgagagtttt gtaaccggac tcggttgcaa ttgccgtacc tcaagccgtt ccggtttaac
     8521 catagaaatt ccgatctttg tggcctacgg taaaaaaacc gtcgaccaaa agccggttta
     8581 gttaaccatt caatatcagc aaggggtgat tgatgatggg gatgacgatt acccaaaaga
     8641 tcctggccgc ccatgccggg aagaaaaccg tgacacccgg cgagttaatc aatgcccggg
     8701 tggacctgat cttgggaaat gacatcactg cgccggttgc cattcgggaa ttttacaaaa
     8761 tcggagtcga taaggttttt gatcccgaca ggatcgccct ggttccggat cacttcgtgc
     8821 cgaataagga tatcaaatcg gcggaacaag cccggatgat gcgcgagttt gcccgcaaac
     8881 accggattac caactacttt gaggtcgggt cgatgggggt ggagcattgt ctccttcccg
     8941 aacaaggcct ggtacttccg ggggaactca tcatcggtgc cgattcccat acctgcacct
     9001 atggggcgtt gggggctttc gccaccggca tcgggagtac cgacatggca gctgcgatgg
     9061 cgctgggcga ggcctggttt aaagttcccg agagcatcaa gttcatttac cacggaaatt
     9121 tacccaagtg gatcgggggg aaagatttaa tcctattcac catcggtcag attggagtcg
     9181 acggcgccct ttaccaggca atggagtttg gcggagaggc tattgccgca ttacccctgg
     9241 agggccggct gaccatggcc aatatggcgg tggaggcggg cgccaaaaac ggcatcttcc
     9301 cggtggatga ccggaccctc gaatatcttc actcccgggc cacccggagt tattcggttt
     9361 ttgaaagcga ctgcgacgcg gtttatgcca aagtctttga atgggatatc tccgggcttg
     9421 aaccccaggt cgccctaccc catttacccg aaaaaactcg gccggtctcg gcgtgccgtg
     9481 ggatcaatct cgaccaggta atcatcggtt cctgcaccaa cggtcggatt ctcgatctcc
     9541 gtgaggccgc ggcagtcctc aaaggccggc atgtcaaccc taacgtgcgg ctgattatca
     9601 tccccggaac gccggccatc tggcgccagg cgatgcggga aggtttgttt gaagtcttta
     9661 tggatgccgg cgccgctatc tccacgccaa cctgcggtcc ctgcctgggt ggacatatgg
     9721 gaatactggc caaaggtgaa cgggcattgg ccactaccaa tcgtaatttt gtaggccgaa
     9781 tgggtgatcc cggtagcgaa gtctatctgg ccggcccggc tgttgccgcc gcttcggcga
     9841 taactggagc cattgctggc cccgaggagg taggagcatg agtttattcc atggaaacgc
     9901 ctggaccttc ggtagcgata tcaataccga cctgattatt cccgcccgtt acctgaatac
     9961 cagcgatccc cgggagttgg ccctccactg tatggaagac gccgacccgg gatttcccgc
    10021 taaagtgcga cccggcgata tcttggttgc cggaaaaaac tttggttgcg gtagctctcg
    10081 ggag
//
LOCUS       DASVMB010000044         8264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_79179,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000044 DASVMB010000000
VERSION     DASVMB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79179"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF531_01505"
     CDS             complement(<1..491)
                     /locus_tag="VF531_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7712671.1"
                     /translation="MLELLKTLLPNVVQLYPDLIKALLETLLMVWVAGTISSLIGIPV
                     GVTLVVTRAGGILEHRVLNGVIGKIINIMRSIPFIILLAAIIPLTRLLAGTTIGIKGA
                     MVPLIFGAFPFIARQIESTLLDIDKGVIEAAQAMGSGPYEIIWRVMFIEGLPGIIYAL
                     TIAT"
     gene            complement(439..1518)
                     /locus_tag="VF531_01510"
     CDS             complement(439..1518)
                     /locus_tag="VF531_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7712672.1"
                     /translation="MIQLLDITKTYTAENGRIEALQGVTLHINAGEIFGIIGYSGAGK
                     STLIRCMNLLERPTSGKVIVNGRDLTALSPGELRQTRAKMGMIFQNFNLMKSRDVFKN
                     IAYPLQGKGLSKAQIEQKVLNLLKLVGLSEKVHAYPRQLSGGQKQRVAIARALANDPQ
                     VILCDEATSALDPQTTQSILRLLREINQKLKITVVVITHEMQVIKEICHRVAVLEDGK
                     IAEQGELLQIFAHPKAKATRDFIATVFQSNKLYELLESKAFLRSIAEDEILARVSFIG
                     QRTNQAFVSKISRLFMIDASILFGNIEMIREVPVGNLVVKLSGEYEDISKAIQYLNDH
                     EIFVEVIKGAGASQNTAPQRSAAIS"
     gene            complement(1733..2746)
                     /locus_tag="VF531_01515"
     CDS             complement(1733..2746)
                     /locus_tag="VF531_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712673.1"
                     /translation="MNSGAEKLISVRQLKKYFRVKSRFLQPEKRWLRAVDGVSFDIHR
                     GETFGLVGESGCGKSTLGRTIVRLYEPTGGEIYFDGTDITRLSRKELRPQTRESYRRR
                     IQVIFQDPYSALNPNMNLAELIDEPMAIHTNLSKPERMEKIEVLLAKIGMKREDMEKF
                     PWEFSGGQRQRIGIARALSVEPEFILCDEPIAALDVSIQAQVVNLLEDFQEEFKLTYL
                     FIAHDLSMVRHISDRIAVMYLGRIVEIAESDELYRNPLHPYTQALLAAIPIPDPRKAR
                     IGSGRLIGIEPPNPLDLPGGCRFHNRCPEATGLCRDQEPELEEVADGHFLACHLVHGP
                     HCG"
     gene            complement(2743..3759)
                     /locus_tag="VF531_01520"
     CDS             complement(2743..3759)
                     /locus_tag="VF531_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712674.1"
                     /translation="MMNQLRDEISHEPSLLEVNDLRTSFYDRGQWITAVRGVSFQVQS
                     GEIVGIVGESGSGKSVLMKSILDILPANAWVEGGEIIFGARHLEKLTPHAKRAIRGRE
                     IAMIFQDPMTALNPLKTIGAHLEELSIRYQRLSRSQARIKALESLRMVGIPAPERRLK
                     QYPHEFSGGMRQRVMIAMALTCGPKLLIADEPTTALDVTIQAQILELLQGLQTRHRMA
                     VILISHDLGVVASLCNRIIVMYAGLIMEEGTADEIFYHPRHPYTKALLNSIPRLDAPA
                     QNRLQPIRGTAPSLAAPLKGCPFAERCEQAMELCFQRMPKRSQSSPTQSWICFRAEKG
                     GVEI"
     gene            complement(3756..4616)
                     /locus_tag="VF531_01525"
     CDS             complement(3756..4616)
                     /locus_tag="VF531_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712675.1"
                     /translation="MKNRRIEKFQDELRSNKAARLGVIFLVLISLLAVFAFLSPYQPD
                     AADVAAMFQHPTAVHWFGTDELGRDYLTRTIYGGRISLAVGLLAMIISTGIGVAVGTV
                     SGYFGGKLDNFLMRTVDVISSIPWMILVTVTSIYLTPGLFTVVLVISLFTWMNTARLI
                     RAETLSAKEREYVLYAVSTGQSTFKIILKHIVPTVFPTLIVAATVNIANAIMMESALS
                     FLGLGVQQPLSSWGSLLQNAQGNLENAPYMAVLPGVLIILTVYSFNKLSQALRVLVEP
                     KSGGNRQGVK"
     gene            complement(4619..5590)
                     /locus_tag="VF531_01530"
     CDS             complement(4619..5590)
                     /locus_tag="VF531_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712676.1"
                     /translation="MAKYIIKRLAQAIPLLILISILCFTLLKLAPYDAIDAISTPNMS
                     QRTIALIKAKYGLDRPAYLQYFYWISGIVRGEFGYSLVTHESIAEALLARIPATVLLV
                     LPAYAAALILAVILGLYSGFQQGKWLDKFVDGCCSIGIATPSFWLAMILVFIFGYKLK
                     WFPIIGMHTIGAEHSLLDLVRHLVMPFTVLTIAFLPELIQYVKSSTISQLSEDYVVVQ
                     KAFGAGNVEILFRHVFKNVLLPVITMIGMALPMLVTGAFITETVFSWPGIGPYFVKAI
                     QGMDYPVVMAIMLLSASMVIIGNLVADLLYSIIDPRIQSIQVQRIGG"
     gene            complement(5712..7277)
                     /locus_tag="VF531_01535"
     CDS             complement(5712..7277)
                     /locus_tag="VF531_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712677.1"
                     /translation="MRREKLAGIIFIAALFVSIFTTPYASGTVFAASKPSQFIYGIDG
                     DPGNSVNVITTGDRYGLMEIKVLYSPLYMYNGAGKVVYFLAESMTPAPDYLSYTAKLR
                     KNVKWSDGRPFTADDVIFTYEQMLKESNGGWARSQLIFDGQPVKVAKVDAYTVKFTLP
                     KVSIPALELLGNIFIMPKHIYEGEGNIENSPKNAAPVGTGPYKLQEYKAGQYVKFVKN
                     GDYFLGAPKIDTIVFRIIGDANTALLALKSGEINALAVQPTDIGKLQGGSRIQVIPYS
                     EGRIGYLAFNLTSQTVQNKLVREAVAYALNRNEIITASLVSSKYALPAYSFLPSTATY
                     HTDNVEKHPFNLKKAKDLLAKAGIRNLRLKLAYSGNNVQQQKQAAIIQQNLKAAGINL
                     ELAGMDSSALSAKLKTVQTDFDMYLNGYIMGIDPDTFNSLFVTGDPSNFMHYSNSEVD
                     RLFQQGRVEKDQAKRKAVYEKIQQLIAADVAFYPITENKRILAIDSRIGGIAQAGLVP
                     VYTFEDTSKLFFK"
     gene            complement(7513..7896)
                     /locus_tag="VF531_01540"
     CDS             complement(7513..7896)
                     /locus_tag="VF531_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX7712678.1"
                     /translation="MKTRVAVASSDGKYVNQHFGRATQFLIFEMGHDQDREDNDNPMD
                     PGDHCEYRFIELRRNEPSCPGEDLEENKHLHVVRLLQDCQAVLVSRIGTGAMEILQEN
                     GIEPYVIPDYIEDALKRFDQLKRSS"
     gene            8081..>8264
                     /locus_tag="VF531_01545"
     CDS             8081..>8264
                     /locus_tag="VF531_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S58 family protein"
                     /protein_id="HEX7712679.1"
                     /translation="MLPRIGLKIGHYTDDAALTGVTVFLAEQGAEIGIDIRGSSTGTI
                     NTPAYDPKSSGKLVHAV"
BASE COUNT         2058 a         2176 c         1997 g         2033 t
ORIGIN      
        1 gtggcgatag taagcgcata gataattccc ggcagaccct cgataaacat cacccgccag
       61 atgatctcat acgggcccga tcccatggcc tgcgccgctt caataactcc tttatcgata
      121 tccagcaggg tggactcgat ctgtcttgcg ataaacggaa atgcgccgaa tatcaagggc
      181 accatcgccc ctttgattcc aattgtcgtt cccgccagca gtcgggtgag cgggataatg
      241 gcagccaaca gtatgatgaa gggaatcgaa cgcataatat tgattatttt gccgatcacg
      301 ccattcagaa ccctatgttc caaaataccg cccgctcttg tcaccaccaa agtgacaccc
      361 accggaattc caatcaggct tgagatggtt cctgcgaccc acaccatgag tagagtttct
      421 aataacgcct taatcaggtc aggatatagc tgcactacgt tggggagcag tgttttgaga
      481 agctccagca ccttttatca cctcgacgaa aatttcatga tcgtttaaat attgaatcgc
      541 cttggatatg tcctcatact ctccgctcaa cttaacgacc aggtttccta caggtacttc
      601 ccggatcatc tcgatattgc cgaataaaat actggcatcg atcataaaca gcctggatat
      661 cttggacacg aaagcctgat tggtccgttg cccgataaag gagacccgag ccaggatttc
      721 atcttcagcg atggatctga gaaatgcctt gctctccaac agctcataca acttattgga
      781 ttgaaatacc gtggcaataa aatccctggt tgctttcgcc ttgggatggg caaaaatttg
      841 aagcagttca ccctgttccg cgattttccc gtcctcaagc acagccaccc ggtggcagat
      901 ctctttgatc acctgcatct cgtgagtgat caccaccacc gtgatcttca gcttttggtt
      961 gatctcccgc aacagccgta aaatagactg agtcgtctga gggtccaaag cggaagtcgc
     1021 ctcatcgcat aagatcactt gcggatcatt ggccagagcc cgcgctatgg ctactctttg
     1081 cttttggccg ccgctgagtt gccgtggata agcatggact ttttcactga gaccgaccaa
     1141 tttcaagaga tttaaaacct tctgttcgat ctgggcctta cttaacccct tcccttgcag
     1201 gggataggca atatttttga atacatcccg cgatttcatc aggttaaagt tttgaaagat
     1261 catccccatc ttcgcccggg tctgtctcaa ctccccggga gataaggcgg tcaggtcgcg
     1321 gccgttcaca atgacctttc cggaagtggg tcgctcaagc aaattcatgc agcggatcaa
     1381 ggtactcttg ccggcgccgc tgtatccgat aattccgaat atctctccgg cattgatatg
     1441 taaagtcacg ccttgtaacg cttcaatccg gccattttcc gcggtataag tcttggtaat
     1501 atccaacagc tgaatcatga aattctcctc caaaaccagt cgcttcacct atcgtttgtg
     1561 tcggaaggag cggcccgatt caataaatta gtagtattcc tatagaaata ctaggttatt
     1621 aatataataa tttcctctgc cctatttgtc aacagtttct tgagctgttt tcaaattgaa
     1681 tatttattgg tttttgcata aacggacgaa tgattgcttg actgatgcca aatcatccgc
     1741 agtggggacc atggaccaag tgacacgcaa gaaaatgtcc gtctgcgact tcctccaatt
     1801 ccggttcttg gtcgcggcat aacccggttg cctcagggca tcggttatga aaacggcatc
     1861 cccccggaag atccagcgga ttcggcggtt caatgccgat gagtcgccca gagccgatcc
     1921 gagccttgcg gggatccggg atggggatgg cagccagcaa cgcctgggta taaggatgga
     1981 gcggattgcg atacaattcg tcgctttcag cgatctccac gatccgaccc aggtacatta
     2041 cggcgatacg gtcggatata tgacgcacca tcgacagatc atgggcaatg aaaagatagg
     2101 tcagtttgaa ctcttcctga aagtcttcca gtaaattgac cacctgcgcc tgaatggaga
     2161 catccagcgc ggcaatgggt tcgtcgcaga ggataaattc cggttcaacg gagagcgccc
     2221 gggcgattcc gatccgctgc cgctggccgc cgctgaactc ccagggaaac ttctccatgt
     2281 cttcccgctt catgcctatt ttggcgagga gaacctcgat cttttccatc ctttccggtt
     2341 tggacagatt cgtatgaatc gccatcggct catcgatcag ctccgccagg ttcatattag
     2401 gattcagcgc cgaataaggg tcctgaaaaa tcacctggat gcggcggcgg taggattcgc
     2461 gcgtctgcgg ccgcagctct tttcgggaca atctggtgat atccgtacca tcaaaataaa
     2521 tctcgccccc ggtcggctcg tataaccgga cgatagtccg gcctagcgtg gatttgccgc
     2581 agccggactc ccccaccagc ccgaaggtct ctccccggtg aatatcgaaa ctaacaccgt
     2641 ccaccgccct gagccaccgc ttttcaggct ggagaaaccg gcttttcacc cggaaatact
     2701 tcttaagttg cctgacagaa atcagttttt ctgcgccgga attcaaattt caacccctcc
     2761 tttctccgct cgaaagcaga tccaactttg agttggagaa gattgactgc gcttaggcat
     2821 cctttgaaaa cacaattcca tggcctgttc acagcgttcc gcaaagggac agcccttcaa
     2881 gggggcagcc aaagaagggg ctgtgccgcg gatcggttgc agacggtttt gcgccggtgc
     2941 atcgagcctc ggaattgaat ttaagagcgc cttggtatac ggatggcggg gatgataaaa
     3001 aatctcgtct gcagtgccct cttccatgat caatccggca tacatgacga taatccggtt
     3061 gcacaggctg gccactaccc ccagatcatg gctgatgaga atcaccgcca tccggtgtcg
     3121 ggtttgcaac ccttgcaaca actccaggat ctgtgcctgg atcgtaacat ccaaggcggt
     3181 agtcggctca tcggcgatta gcagcttggg gccgcaagtg agggccatgg caatcatgac
     3241 ccgctgcctc atcccgccgc tgaactcgtg gggatactgt ttcaatctcc gttccggggc
     3301 ggggatccct accatgcgca ggctttcaag agccttgatt ctggcttggg accggctcaa
     3361 ccgttgatag cggatggata actcttccag atgggcgccg atggttttta aggggttcaa
     3421 ggcggtcatg gggtcctgaa agatcatcgc gatctcgcgg ccccggatcg cccgtttggc
     3481 atggggagtc aatttttcaa ggtgtcttgc gccaaagatg atctcgcccc cctctaccca
     3541 agcgttagca ggcaggatat ccaaaatcga cttcatcagc acgctttttc cgctgccgga
     3601 ctcacctaca atccctacga tttcaccgct ttgcacctgg aaactgacac ctcgtaccgc
     3661 ggtaatccat tgaccccgat cataaaaact ggtccgcaga tcattaacct caagtaaact
     3721 tggttcatgg gatatctcat ccctaagctg attcatcatt tcaccccttg gcggttaccg
     3781 ccgcttttcg gttctaccag gacccggagt gcctgactga gcttgttgaa ggaataaacc
     3841 gtcaggatga tcaagacccc cggcaaaacc gccatatatg gcgcattctc caaattgccc
     3901 tgggcattct ggagcaggct gccccatgaa gaaagcggtt gctgaacgcc caacccgaga
     3961 aagcttaagg ccgattccat cataatcgcg ttggcgatat tcaccgtcgc cgccacgatc
     4021 agcgtaggga aaaccgtggg aacaatgtgt ttcaggatga ttttgaacgt ggactgaccg
     4081 gttgatacgg catagagtac gtactccctc tctttggcgg agagcgtttc cgcccggatc
     4141 agcctggctg tgttcatcca ggtgaaaagg ctgatcacca aaacgaccgt gaacaatccc
     4201 ggggtcaaat aaatgctggt tacggtgact aaaatcatcc aggggatgga ggagatcaca
     4261 tccacagtgc gcatgaggaa gttgtccaac ttccctccga aatagccgct gactgttccc
     4321 acagccaccc cgatgccggt agagatgatc atcgccagca atccgacggc gagggagatc
     4381 cgccccccgt atatggttcg ggtcagataa tcccggccca actcatccgt gccaaaccaa
     4441 tggaccgcgg tggggtgttg aaacatcgcg gctacatccg ctgcgtcggg ttgataggga
     4501 gataggaagg caaagacggc caacagggag atgagaacca gaaatatcac tccaaggcgc
     4561 gccgccttat tggatctcaa ctcatcttga aatttttcaa ttcgtcggtt cttcatcttc
     4621 atcctccaat cctttgaacc tgaattgact gaatgcgcgg atcaatgatg ctgtataaaa
     4681 ggtccgccac caagttgccg ataatgacca tgctggcgga taagagcatg attgccatca
     4741 ccaccggata gtccattcct tggatagcct tgacgaaata aggtccgatc ccgggccagc
     4801 taaaaaccgt ttccgtgatg aaagcccctg tgaccagcat cggtaatgcc atgccaatca
     4861 tcgtaatcac cggcaagagc acattcttga atacatggcg gaaaagaatc tcaacattgc
     4921 cggctccaaa agccttttga accacgacat aatcctcgga aagctggctg atggttgagg
     4981 acttcacata ttgaatcagc tccgggagaa aagcgatggt aagaaccgtg aacggcatca
     5041 ccaaatgccg caccaaatcg aggagggaat gctccgctcc gatggtatgc atgccgatga
     5101 tgggaaacca cttgagtttg tacccgaaaa tgaagactaa aatcatggcc agccaaaaac
     5161 tcggagtggc aatgcctatc gagcagcatc cgtcaacgaa tttatccagc catttgcctt
     5221 gttgaaaccc ggaatacagg ccgagtataa cggcaagaat caatgccgcc gcataagcgg
     5281 gaagtaccag aaggacggta gccggaatac gggcgaggag agcttcggcg atgctctcgt
     5341 gggttaccag ggaataaccg aactcgccgc ggacgatccc ggagatccaa tagaaatact
     5401 ggagataagc cggccgatcc agcccatatt tcgccttgat caaggcaatc gtccgctgtg
     5461 acatattggg tgtcgagatg gcgtcgattg cgtcataagg ggccagcttc agtagggtaa
     5521 aacatagaat ggatattaaa atcaaaagcg gtatggcctg cgccaatcgc ttgatgatgt
     5581 atttggccaa acggatttca cttccctatc gttgcgaaat aaatatgaat gaagataaac
     5641 ggcaaccgtt taccttcatt caaccggtga acttccgcaa atccttgatt ccccgcggat
     5701 aggcccgcag tttacttgaa gaaaagcttt gacgtgtctt caaacgtgta taccgggacc
     5761 agaccggcct gggcaatgcc tccgattcgg gaatcaatcg ccagaatccg tttattctcg
     5821 gtgatcggat aaaaagcgac atcggcggcg atcaattgct ggatcttctc gtagacggcc
     5881 ttacgtttgg cttgatcctt ttcgacacgg ccttgctgga aaagccggtc cacttcggaa
     5941 ttgctgtaat gcatgaaatt actgggatct cccgtgacaa aaagggagtt aaaagtatcc
     6001 ggatcgatcc ccatgatata tccgttgaga tacatatcaa aatccgtttg taccgtttta
     6061 agtttggccg ataaggcgct tgaatccatg cccgccagtt cgaggttgat ccccgcggcc
     6121 ttcaagtttt gttgaatgat cgccgcctgt ttttgctgtt gtacattgtt gccggaatat
     6181 gccagcttca gccggagatt acggataccg gctttggcca gaagatcctt ggcttttttc
     6241 agattgaacg gatgtttctc gacattatcc gtatgatacg tggcggtgct cggcaggaac
     6301 gaataggcgg gcagcgcgta tttggacgat actagactgg cggtaataat ctcgttccga
     6361 tttaacgcgt aagccaccgc ttcgcgcacc agcttgttct gaaccgtttg ggaagtcaaa
     6421 ttaaacgcca aatatccgat tcttccttca ctatagggaa tgacttgaat ccgggaaccc
     6481 ccctgcaatt tgccgatatc ggtcggttgc acggctagag cgttgatttc cccgcttttt
     6541 agcgccagga gggcagtatt ggcgtcaccg ataatccgga agacaatggt gtcgatcttc
     6601 ggcgcgccta agaaataatc gccgttcttg acgaacttaa catactggcc cgctttatat
     6661 tcctgcaatt tatacggccc ggtacccacc ggggcggcgt ttttggggct gttctcgata
     6721 ttcccctcac cctcataaat atgtttgggc atgatgaaga tattgccgag cagctccagc
     6781 gcgggaatac ttaccttcgg cagcgtaaac ttgaccgtgt aggcatcaac cttggcaact
     6841 tttacgggtt gcccgtcgaa gatcagctgg cttctggccc aaccaccgtt agactccttc
     6901 aacatctgct catatgtaaa aatcacatca tccgcagtaa aaggccgccc gtcactccac
     6961 ttgacatttt tccggagttt agccgtataa ctcagataat ccggcgccgg ggtcatgctt
     7021 tctgccagaa aataaaccac cttgccggcc ccattataca tatagagcgg ggaatagagc
     7081 actttgatct ccatcaggcc gtatcggtcg cccgtggtga tcacattcac ggaattgccg
     7141 ggatcaccgt ctatcccata aataaactgc gacggtttgc ttgcggcaaa aaccgtgcct
     7201 gaagcatatg gggtcgtgaa gatcgataca aatagagccg caatgaaaat gattcctgcc
     7261 aatttttctc gtctcatctt ttttctcctt aggttggttg atttgaatag aatttaaagt
     7321 attcctatag gaataatatg aattaattat aaatgcgatt tccccaaaaa tcaatagatc
     7381 ttggaaagtc ttacctgata attatcactt tttttgaatc cggagttttc ccgaaacacg
     7441 cgcaacccgt tcacgaaagg atgaataaaa agggggaatc gatcatgagg cggtttaaag
     7501 gggaacgcat ctttaacttg atcgcttgag ctgatcgaat ctttttaaag catcctcgat
     7561 atagtccggg ataacatacg gttcgatccc gttttcctgt aagatctcca ttgcgccggt
     7621 accgatccgg ctgaccagta ccgcctggca gtcctggagt agacggacca catggagatg
     7681 tttattctct tcaaggtctt ctcccgggca cgacggttcg ttccgtcgca actcaataaa
     7741 ccggtattcg caatgatcgc cgggatccat tggattatca ttgtcttcgc gatcttgatc
     7801 atgccccatc tcaaagatca aaaattgggt cgccctcccg aaatgctggt tgacatactt
     7861 cccgtcactg ctagccacag ctacacgagt tttcattttt tgatgccctc ccggaatcga
     7921 ttttggcaca ttgtacagtc accaagataa ttggtcaagg ccgggaaaat gccaatccgg
     7981 cgattccttc ccgaaatctt gcatcggaat atgttttatg ctatattcta ctaaaatgat
     8041 atatttgcta agaaatggag ccgccatggc gcatcaacaa ttgttaccga gaattgggtt
     8101 gaaaatcggg cactacaccg atgacgccgc actgaccgga gtcactgttt ttctggccga
     8161 gcaaggcgcg gagatcggca tcgatatacg ggggtccagc acaggcacga tcaacacccc
     8221 ggcttacgat ccgaaatctt cgggaaaact cgttcacgcg gtgg
//
LOCUS       DASVMB010000045         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_446686,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000045 DASVMB010000000
VERSION     DASVMB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446686"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF531_01550"
     CDS             <1..762
                     /locus_tag="VF531_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7712680.1"
                     /translation="LCEKPPAISKVDAEKMAQTAKENQKILAYNFHFRQSEEVQSIKR
                     FIDGGEFGEIYYVRVKALRRRGIPGWGVFTNKEIQGGGPLIDIGVHMLDTALYLMDFP
                     AADYVSASAYQKIGTRAGVGLMGSWDPNRFTVEDSAFGFIRFKNGATLVMETSFALNI
                     KERSLMNVELCGEKAGASVFPPVIFTEKHGSLLDMELPYVAEVDKHHQSIKSFIDVCM
                     GYEGLICTAEQGLAIQKIVDALYRSAELGKPVTLD"
     gene            775..3096
                     /locus_tag="VF531_01555"
     CDS             775..3096
                     /locus_tag="VF531_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003396838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX7712681.1"
                     /translation="MLNEASGKSQEQNWMIEETCFNERYQGKYESILALGNGYLGLRS
                     AMEEPYIGQTRNLFVAGTYDKFDATEVTELPNAADLTEMFIHFNDEPFSLKNGKVLEY
                     SRKLNLKTGELTRSIVWENSRSERYQMTFGRFVSLDDRHLIGIKVDIVPLTVPAEITV
                     KTGINGRQTNSGVQHFHDGDKRVYEDRYMQLIQTTIESNIGFVINSRVRLYIDNKEVV
                     NPGGYSLERRSIYQNSKISIDPGATLTIEKLSNVFTSLDSDIGTGLRVEGLIKHSLEH
                     LSEVCKMRYDSLLANSKAKWQKYWEEARVEIQTSDAFDQLAVRFAQYHLLIMTPFHDE
                     RFSIGAKGLTGEGYKGHVFWDTEIFILPYFQHTFPAIARTLLRYRFNTIDGARNKAHQ
                     FGYKGAMYPWESALSGAEETPEWAAINIMTGKATRIWSGIKEHHVTADIAYAAWNYYL
                     STVDEDFMNEFGSEIIFECAEFWYSRLEWSEAKKAYHIHDVIGPDEYTEHIDNNAYTN
                     YMAEFTIRTAIQLYELGIRKNWSHFQHIVNQLRLEERMARYRGVVDRIYLPQPDADDI
                     IPQDDTFMSKGCINLEKYKRSDEKQTILKEYTREQVGDLQVLKQADVVMLLYVLRNRF
                     SPKVKQANWEYYEERTIHDSSLSAAVHGIVANDFNCSEAAYRFFQKAARIDLGPNPVS
                     SNDGIHAASMGGIWLAVVMGFGGVSNDNGTLHLEPKLPEPWERLSFNLWWQGEKLHLE
                     ITKTRIKITKASVTGMDLIVKGNRYTLLSELTL"
     gene            3118..>3817
                     /locus_tag="VF531_01560"
     CDS             3118..>3817
                     /locus_tag="VF531_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7712682.1"
                     /translation="MPIIFDRDSLTFHLQAGDSSYIFKVYREGYLVHLYWGRKIRSHD
                     LSRLIPYGRKPLSPTPDPLDEGFSLDTVPQEYPAFGNSDFRTPAYQIQLENGSTITDL
                     RYKGHRIFKGKPKLFGLPATYVESPLDAETLEIDLLDEVSGLRAILSYTVFENYPAIA
                     RTVKLVNCGNENLKILRVLSANVDFHDADYEFLYLSGAWGRERHIERRALTQGSQSIE
                     SKRGSSSHQQNPFAA"
BASE COUNT         1079 a          791 c          966 g          981 t
ORIGIN      
        1 ctgtgtgaaa aaccaccggc aatttcaaag gttgatgcgg aaaaaatggc tcaaacggcc
       61 aaggaaaatc aaaaaatcct agcttacaat tttcactttc gacaaagtga ggaagtacag
      121 tctatcaaac gttttatcga cggtggtgaa tttggcgaaa tatattatgt cagagtcaaa
      181 gcgttaagaa ggagagggat tccgggctgg ggggtcttta ccaataagga gatccagggg
      241 ggagggccgt tgattgatat tggcgttcac atgttggata ctgctcttta ccttatggat
      301 tttcccgcag ccgattacgt gagcgcttca gcctatcaaa aaattggcac tcgagccggg
      361 gttggattga tggggagttg ggatccaaac cgatttacgg ttgaagattc ggcgtttgga
      421 tttatccgct tcaaaaacgg cgcgactttg gtaatggaga cctcgtttgc cttaaatatc
      481 aaagaacgtt ctctcatgaa tgtggaatta tgcggagaaa aggcgggagc ctcggttttt
      541 cccccggtta tcttcactga gaaacacggc agtcttttgg acatggagct cccatacgtt
      601 gcggaggtgg ataaacatca tcaaagtatt aaaagtttta tcgatgtttg tatgggatat
      661 gaaggattga tttgtacagc ggaacagggt ttggcgatcc agaagattgt cgatgccctt
      721 taccgatcgg ctgaattagg gaaaccggta actctggact gaggtgattt tgcggtgctg
      781 aatgaggcaa gtggcaaaag tcaggaacag aactggatga tcgaggaaac ctgcttcaat
      841 gaaagatatc aaggcaaata tgaaagtatt ttggctcttg gcaatggtta tctggggcta
      901 aggtcggcta tggaagaacc ctatattggc caaaccagaa acctgtttgt ggcagggacc
      961 tatgacaagt ttgatgctac cgaagtgacc gagcttccaa atgcggcgga tctaacggaa
     1021 atgttcatac attttaatga tgaaccgttt tccctcaaaa acggcaaggt tcttgaatac
     1081 agccgcaagc tgaatttaaa aaccggggaa cttaccagga gcattgtttg ggaaaactcc
     1141 agatctgaaa gatatcaaat gacttttgga aggtttgtct ccctggatga tcgccacctt
     1201 atcgggatca aggttgacat cgttcctttg accgttccgg ccgagataac cgtcaaaacg
     1261 ggaatcaatg gacgccaaac caactccggg gtccagcatt ttcatgatgg cgataagcgg
     1321 gtttatgaag atcgatatat gcagttaatt cagactacca ttgagtccaa tattggtttt
     1381 gtgatcaaca gtcgggtgcg gctttatatt gacaacaagg aagtcgtaaa tcccgggggg
     1441 tattcgctgg aaagacgcag tatttatcag aactccaaaa taagtattga tccgggagca
     1501 acactaacca tcgaaaaatt aagtaatgtc ttcaccagcc tcgacagtga tatcggtacc
     1561 gggcttcgtg tagagggctt aatcaaacac tcattggaac accttagtga agtttgcaaa
     1621 atgagatatg atagtttatt ggccaatagt aaagccaagt ggcagaaata ttgggaggaa
     1681 gcccgggtag agattcaaac ctccgatgcc tttgaccaat tggcggttag gtttgcccag
     1741 tatcatctgc tgatcatgac tccttttcat gatgagcgtt tcagtatcgg agccaagggg
     1801 ctcacgggag agggttataa aggacatgtg ttttgggaca ccgagatctt tatcctgccg
     1861 tattttcagc atacctttcc tgctatcgcc cgaacattgc ttaggtaccg ttttaacacg
     1921 attgatggcg ccaggaataa agcgcatcaa tttggttaca aaggagccat gtacccctgg
     1981 gaatcggctt tgagcggcgc ggaagaaacc cccgaatggg cggcgatcaa tatcatgacc
     2041 ggaaaagcaa ccaggatatg gtcgggcata aaggaacacc atgttactgc ggatattgct
     2101 tatgctgcgt ggaattacta tttgtccacc gttgatgagg acttcatgaa tgagtttggc
     2161 agcgaaatta tctttgaatg tgccgagttc tggtacagcc ggctggagtg gtccgaagct
     2221 aaaaaagcat atcacattca tgatgtcatc ggacccgacg agtatacgga acatattgat
     2281 aataatgctt acaccaatta catggcggaa tttacaatcc ggaccgccat tcaattatat
     2341 gaacttggga tcaggaaaaa ctggagtcat tttcaacata tcgtcaatca gctccgtctg
     2401 gaagaaagaa tggctagata caggggcgta gtcgaccgaa tctatctgcc gcagccggat
     2461 gcggacgata ttattcccca agatgatacc tttatgagta aaggttgcat caaccttgaa
     2521 aaatataagc gttcggatga aaaacaaacg attcttaagg aatacacccg ggaacaagtc
     2581 ggtgatctgc aggtgttaaa gcaagccgat gttgtgatgc tgttatacgt tttgcggaac
     2641 cgtttttcgc caaaagtaaa acaggccaac tgggagtatt atgaagagcg gaccattcat
     2701 gattcttctt taagtgcggc ggtccacggc atcgtggcga atgacttcaa ttgctccgaa
     2761 gccgcctacc gttttttcca aaaagcggcg agaattgatc tgggaccgaa tccggtctct
     2821 tcaaatgatg ggattcacgc ggcatccatg ggcggcattt ggctggcagt ggtcatggga
     2881 tttgggggag tctccaatga taacggtaca ttgcaccttg aaccgaaact gccggaacca
     2941 tgggaacggc ttagtttcaa tttatggtgg caaggtgaaa agttacattt ggagatcacc
     3001 aagacccgga taaaaataac taaagcctcc gtgacgggga tggatttgat tgtcaaagga
     3061 aacaggtaca ctttgctttc ggaacttaca ctataaaatg tggattggag aaacaccatg
     3121 ccgattattt ttgatcggga ttcgctgacc tttcatttgc aggccggaga tagcagctat
     3181 attttcaaag tttatcgcga aggatattta gtccacctct actggggcag aaagatccga
     3241 agccatgatt tatcccgcct gataccttat ggacgaaaac ctctctcacc aacgccggat
     3301 cccctagatg agggattttc gttggatact gttccccagg aatacccggc cttcggcaat
     3361 agcgatttcc gcaccccggc ctatcaaata cagctggaga acggttctac catcaccgac
     3421 ttgcgctata aggggcatcg gatttttaaa ggtaagccga aactgtttgg ccttcccgca
     3481 acctatgtag aaagtccgct agatgctgaa accctggaaa ttgaccttct tgatgaggtt
     3541 tccgggttaa gggccatcct ttcctataca gtgtttgaaa attaccctgc catcgcccga
     3601 acggtaaaac tagtaaactg cggaaacgag aatttaaaaa tccttagagt gttaagcgcc
     3661 aatgttgact ttcatgatgc cgattatgag tttctttact tatccggggc ttgggggcgg
     3721 gaaaggcata tcgaaagaag ggccttgaca caggggtctc agtctatcga gagcaagaga
     3781 ggctccagca gccatcaaca gaatccgttt gccgcac
//
LOCUS       DASVMB010000046        10156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_152004,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000046 DASVMB010000000
VERSION     DASVMB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152004"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VF531_01565"
     CDS             complement(<1..1037)
                     /locus_tag="VF531_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose phosphorylase"
                     /protein_id="HEX7712683.1"
                     /translation="MEQPERTNYLYFPLANDSGMISAVTPLLHGDLKTGQNSFLLAPV
                     TAEDLHNSRSARNFWIFIEEYGAWSVGGNSPRQMMLNFSDTPEAVTLEAGLLWHKITR
                     TNPELGITAEITNFVPATADTVELMRVRITNTGSAPVRFTPTAAIPIYGRSATNIRDH
                     RHVTSLLHRIRTTPSGVVVKPTFCFDERGHLLNRTNYGVFGVTGTGGKPVGFFPKVES
                     FIGAGGSFEWPEAVVRNRVDFCRAGEEFAGWEAVGAIRFREESLEPGASCSYIMVMTI
                     SEAETDLEYFVEKFGTDEAFEQYLRENKAFWDRKLSSLVFNSGDSDYSNWLRWVGIQP
                     ILRRICGCSFLP"
     gene            complement(1159..2205)
                     /locus_tag="VF531_01570"
     CDS             complement(1159..2205)
                     /locus_tag="VF531_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7712684.1"
                     /translation="MATLKDISRAAGVSVGTVSRVINHSPNVSEAKRQKVLRIMEEMS
                     YQPNFMAQVLSRKGSKTGELGLVVTGIDNPANVEIVRGATEEANHVGYTVMLCTAWST
                     PEITRYLEVFIRRRVEGVAIASYMDSRTLLSVSKLQTHGIPLAVSRDAGWPIAKDDGG
                     LNRLPIATVDFESAHSCEQAIQYLIGLGHQQIAVISGNAELNENDPRLLGYKNALEKA
                     GLEFNPELLFPGGTDTIMTGVHSMQEILATRRDITAVFGFNDLLAIGAMRALQEAGVR
                     VPEEISVIGFDNILMSSFVNPPLYTINVPKYEIGQALIRELIGVIQGTAQGHELLGTN
                     FVVRQSSGFVRTRT"
     gene            complement(2409..3293)
                     /locus_tag="VF531_01575"
     CDS             complement(2409..3293)
                     /locus_tag="VF531_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712685.1"
                     /translation="MSNTTTIRERIFMGSNPATRGKRSIADVCREIFLFLIALCCIFP
                     LLWMISTALKGTEEAFTSFNLIPKVWRFDNFKVAWEQANFSLYFFNSTVYTISVMFGV
                     WLAATLGAYGFARLQMPGKKVIFYILIGSLMVPIPGSFIPLYLMMNSYHLAGTRLGYI
                     LPMISSGLATSIFILKSFFESIPKEFEESARLDGCSKLGIFSRIMIPLSRPAWVTVLI
                     FTALATWNDYLWALIMFSKKDLMPIQVGLRVFQGQYFTRYEMLMAGTAIAALPVIVLF
                     IIFQRNIVSGVTAGGIKG"
     gene            complement(3375..4283)
                     /locus_tag="VF531_01580"
     CDS             complement(3375..4283)
                     /locus_tag="VF531_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712686.1"
                     /translation="MAQHLTESKTWNRLKDYCGNYLFILPVVLIFSAFSLYPILRTIQ
                     LSLFKWNGIDPQMTWVGLQNFYVIFAMDPNWWTAMWNATKFAILGVLVMQSLSLGLAF
                     LVDRRSKQSDFYKAVYYIPTILSPMVVGYVWKWIYDPNGGVLNFLLDKVGLHILTRSW
                     LSDPGLALFAVSAASIWAGFGYSFVLFLAGLKGISADIYEAAKVDGANGWKQFWYITL
                     PMLRPILTVVTILTILGAMQLFPLVDAMTNGGPGFATQVPVKSIYDECFKNYHYGYAS
                     AYSVIFGLILLIISMTQMEISRRYDV"
     gene            complement(4351..5649)
                     /locus_tag="VF531_01585"
     CDS             complement(4351..5649)
                     /locus_tag="VF531_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7712687.1"
                     /translation="MKKSLLTVLLVAVVLTAILAVPFASAAPKTITFWHYMVDRNDLL
                     QSFAQQYEKATGVKVDVQLFPGDQDVEKKVEAAIQAGTAPDIYTSNGSADPVKIEGKA
                     RWIKAGGMLNLDKYVTGAWRKNYIPSLLSNISFASGNQYGVEPGLYGLPLDSVNMQIL
                     YNKELFQKAGLDPNRPPKTWAQFIAAGKKLKAAGITPFAGGFGTWLGISIWEVYAWNI
                     MGEKGMIQAQNNGKRFTDKNWVKTFQIFADMRDAGMYADGMATWDNPDAERLFAQEKV
                     AMLYDGSWGIGVIKSANPNLVPKVGAFLPPSAGRYPQYIQGGYGVVLAVNAVSPNKDE
                     AINFLKWLTAPEQQAQYAKTSFNLPASSEVTKKVKLDGGVGDFADDAGRIIPNVGLKG
                     RGGDVDTEITRGIQSIMLKKKTPQQVVDDVNKIWAASLLK"
     gene            complement(5970..7262)
                     /locus_tag="VF531_01590"
     CDS             complement(5970..7262)
                     /locus_tag="VF531_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712688.1"
                     /translation="MLYAIHVHPHDIRDEGAERVVENITELGKIKTIIAEAMTLEERH
                     PYPDGVLPHNPRHSVVVSWATLEVPLSGRLFSGLSIQPVPSAEVLAGNDYIGLLRKAA
                     AVGGATVIPWVKGLNGAFSGRIEDLSVKTLDGQPVPTWLCPSRPETFEYIASLIEGIL
                     DRYPSEAILLDRMRYPDWSGANVQPERMLTCFCDSCCRMMEDEGIDLALLKSVLKRFL
                     KQPDQPGGRFQNLSGAELNEIRKWLGFRAALITRLVDRVSTQVKIYNQTRHMNTKFWL
                     NLWPPSFAWFLGQDYQALGRLCDGAKHFPYHRLGGGADLKGLVEALAIHEDREAQEKI
                     FLMVMELLQIPYAMSFEKFKEFGFPVDFVGRETGIAKQAFGHCKQIFSGIQIWNTPME
                     EIDKACDSARRGDADGFFFYCYGWAELKALQTIGRIIG"
     gene            complement(7325..8839)
                     /locus_tag="VF531_01595"
     CDS             complement(7325..8839)
                     /locus_tag="VF531_01595"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7712689.1"
                     /translation="MMEMDTDFILEMRDIVKKFAGVHALDKVNFLLKPGEIHALVGAN
                     GAGKSTLMKVLAGIYPDYEGTIILHGKEIRFSNPRLALESGIAVIYQEFNLVPQLTVA
                     ENILLGREPLRKLGRIPLLNWPALHDEAAELLAELKFTLPLDVQVAELGVADQQLVQI
                     AKAMATHARVLVMDEPTARLSRTERDNLFEIMRRLKVGGTSIVYISHFLEEVFMIAER
                     VTVMRDGRNAGTALISELNRPELIKMMLGHEVMMDNSKRDTHKDQAILNVEHLSNSAK
                     FQDVSFSLYAGEILGIAGLVGSGRTELARTIFGAEPRKKIRGRIDVNGSIFQATSPKA
                     AISRGIGLAPEDRKQQGLVLIRPVEDNILLSSTSRLRHGWFMDLSARRQLMEKMIQRL
                     SIKCTDSKVLAGTLSGGNQQKVVLAKWLAAKTHILILDQPTAGIDIGTKEEIYRLLRE
                     LADDGSGLIVISDDPEELSRICSRVLIMRKGRMVKELTGNPSSEEVLAGVTAEY"
     gene            complement(8908..9852)
                     /locus_tag="VF531_01600"
     CDS             complement(8908..9852)
                     /locus_tag="VF531_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7712690.1"
                     /translation="MKRIKKSILFVALLAMVFSVSININAAKQYTVGATIWNMSVPFY
                     SNFIKGLHDGAAKYGLKLLLRDGQGDPNTQVAVVRQFIAEKVDMILIVPGDAQAVVPV
                     IQQANAAKIPVISVNNQAGAGAKIVTFVGADDYYFGKQQAKLLTKAIGNSGQVGYIMG
                     QLGTSAQTLRKKGFTDFLKDYPNIKVVSEISDNWDSAKALAAAQDMLSRYAKGSIDAI
                     VCQGPEAVAAAKFTHDSGRDEVRWILGDYPQDVREAIQNGNVYGTVDQDPYPQAVEGM
                     HMSLLYLTGKKSQIPTPNYFLELPLITKDNVSKYNPAW"
     gene            complement(9953..>10156)
                     /locus_tag="VF531_01605"
     CDS             complement(9953..>10156)
                     /locus_tag="VF531_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712691.1"
                     /translation="AIAATFVGGTSTTGGEGSIMKTVLGVLLLGMIANALNLLNVSPY
                     WQPAVTGLIILIAVSLDTAGKRR"
BASE COUNT         2650 a         2693 c         2349 g         2464 t
ORIGIN      
        1 ggcaggaatg agcagccgca gatccgccgc aatataggct ggattcccac ccagcgcaac
       61 caattggagt aatcggaatc gccgctgtta aaaacgaggc tactgagctt ccgatcccaa
      121 aatgccttgt tttcccggag atattgttca aaggcctcat ccgtcccgaa cttttccacg
      181 aaatattcca agtcggtttc ggcctcggag attgtcataa ccatgatgta actgcacgaa
      241 gcaccaggtt ccaggctctc ttcccggaag cggattgctc caacagcttc ccagccggca
      301 aactcctcgc ccgcccggca aaagtcgacc cggttacgga ccactgcttc aggccattca
      361 aaggatccgc ccgcgccgat gaaactctcg accttcggga aaaaaccaac cggtttccct
      421 ccggttccgg tgaccccgaa aaccccgtaa ttcgtccggt tcaaaagatg accccgctca
      481 tcaaaacaaa aagtgggctt gaccaccacc ccggaaggag tcgtccggat ccggtgcagc
      541 agcgaggtga catggcgatg atcccggata ttggttgcgg aacggccata aatgggaatg
      601 gcggcggtgg gggtgaagcg aaccggagcg gagccggtgt tggtgatcct tacccgcatc
      661 aattccacgg tatcagcggt agcgggaacg aaattggtga tctcggcggt gattccaagc
      721 tcggggttgg tgcgggtaat cttgtgccag agaaggccgg cctcaagcgt taccgcttcc
      781 ggggtatccg aaaaattcaa catcatctgg cggggggagt taccccccac cgaccaggct
      841 ccgtattctt cgataaatat ccaaaaattc cgggccgatc ttgaattatg taaatcctcc
      901 gccgttaccg gggcgagcag aaaactgttc tggccggttt ttaggtcgcc atgcagcagt
      961 ggagtaacgg cggagatcat gccggaatca ttggcgagtg gaaaataaag atagttggta
     1021 cgttccggtt gttccatact gaactctccg gagcttcctc taaagatcca ttgagaactg
     1081 tgccttggct catccatgga caggattcct ttccgcggtg atgaaattcc agatcatatc
     1141 cggggtttag tagcggcatt acgtgcgcgt gcgaacaaaa cccgatgact ggcgtaccac
     1201 aaaattggtg ccgagtaatt catggccttg agccgtacct tgaatgactc caatcaactc
     1261 acggattaag gcctgcccaa tctcatattt ggggacgttg atcgtatata ggggcggatt
     1321 aacgaaactg ctcatcagga tattatcaaa accgattacc gagatctcct cgggaacccg
     1381 gactcctgct tcttgcaagg cacgcatcgc tccgatggcg agcaaatcat tgaaaccgaa
     1441 aacagcggtg atatcgcggc gcgtggctaa aatctcctgc atcgagtgga ccccggtcat
     1501 gatggtatcg gtcccccccg ggaataacag ttccggatta aattccagac ctgccttttc
     1561 caaggcattt ttataaccca acagccgcgg atcgttctca ttcaattcgg cattaccgct
     1621 gatcaccgca atctgctggt gaccgagacc aatcaggtac tggatggcct gttcgcacga
     1681 atgagccgac tcgaagtcca cggtggcgat gggtaagcgg tttagacctc cgtcgtcctt
     1741 ggcaatgggc caaccggcat ccctgctgac tgccagagga atgccgtgag tttgcaattt
     1801 actcacgctg agtaacgtcc gggaatccat ataggaggcg atagctaccc cttcaaccct
     1861 gcgacggatg aagacctcca aataccgggt gatctccgga gtgctccagg cggtacaaag
     1921 catcaccgta tagccgacgt gattggcctc ctcggtagcg ccccgcacga tctccacatt
     1981 ggcagggttg tcgatgccgg tgaccaccag gccaagttca ccggttttag aaccctttct
     2041 gctcaagacc tgagccataa agttcggctg ataactcatt tcctccatga tccggagcac
     2101 tttttgacgt ttggcctcgg aaacattggg ggaatggttg attacccgcg aaaccgttcc
     2161 gacggatact ccggcggccc gggagatgtc ttttaaagta gccatgatgt tcctcttata
     2221 aatgagttca atgatacggg attttggcgc gttacgagat gccaccattg cctcgtacgg
     2281 ggccgggaga tgacctactt ttcaaaaacg atacgcaatc atgaatgtca aaaattgagt
     2341 agcgtttaat gattcgcccg gcggtatccg attcctggat ccgataggga aatttttcgt
     2401 tttgattgtc agcctttgat cccgccggcc gtcacaccgg agacgatgtt gcgctgaaag
     2461 atgatgaaca aaacaatcac cggcaacgcc gcaatggctg tcccggccat cagcatttcg
     2521 tatcgggtaa agtattgtcc ttgaaaaacc ctgagcccga cctggatggg catgagatcc
     2581 ttcttgctga acatgattaa ggcccagaga taatcattcc atgtcgccag ggcggtaaag
     2641 ataagcaccg tgacccaagc cggacgggag agcggaatca tgatccggga gaagattcca
     2701 agcttgctgc aaccgtccag ccgggccgac tcctcgaact ccttcggaat gctctcgaaa
     2761 aaacttttca ggatgaagat cgaagtggcc aagccgcttg agatcatggg caggatgtaa
     2821 ccgagacgcg ttccggccag atgataactg ttcatcatca agtacagcgg gatgaaagaa
     2881 cccggaatcg gaaccatcaa agacccgatg agaatataga aaataacctt tttgccgggc
     2941 atttggagcc gggcaaaacc gtaggcgccc agggtggccg cgagccaaac cccgaacatg
     3001 acggagattg tgtacacggt gctgttaaag aagtataacg agaaatttgc ttgttcccag
     3061 gcaactttaa aattatcgaa ccgccagact ttaggaatca ggttgaaact ggtgaacgcc
     3121 tcttcggtcc ccttcaaggc agtggatatc atccagagca acggaaagat gcaacacagg
     3181 gcgatcagga acagaaaaat ctcgcggcat acatccgcaa tactccgctt gccccgtgtc
     3241 gccggatttg aacccatgaa tatcctttcc ctgatggttg tagtatttga catgaaagct
     3301 aacccccttg attgggttac gttcgactga aaatagattt ataaatgcag taaagcttgg
     3361 caagtcgggt ggatctaaac atcatagcgc cttgagatct ccatctgggt catggagatg
     3421 atcaggagta tcaggccaaa gatgaccgaa taagcgctgg cgtaaccgta atgataattc
     3481 ttgaaacact cgtcgtaaat ggactttacc ggaacctggg tggcgaaacc cggtccgccg
     3541 ttggtcatcg catctaccaa agggaacaac tgcatggcgc cgagaatggt cagaatcgtg
     3601 actaccgtca ggattggccg gagcatcggc agggtgatgt accaaaactg tttccagccg
     3661 ttcgccccgt ccaccttggc cgcctcgtag atatcggccg agatcccttt caatcccgcc
     3721 aaaaaaagca caaacgaata accgaaacca gcccagatcg atgccgccga aaccgcaaat
     3781 aacgctaacc caggatcact caaccaggac cgggttaata tgtgaagccc taccttatcc
     3841 aacaaaaaat taagcactcc gccattgggg tcataaatcc atttccaaac gtaaccgacg
     3901 accatcgggg agagaatcgt agggatataa tagactgctt tataaaaatc cgattgctta
     3961 ctgcgtcgat cgaccaagaa cgccaatccc aaagagaggg attgcataac gagcactcca
     4021 agaatggcga atttggttgc attccacatc gccgtccacc agttcggatc catggcgaag
     4081 atgacataaa agttttgtaa tcccacccaa gtcatctggg ggtcgatacc gttccattta
     4141 aatagtgaca attggatcgt ccgtaaaatc ggatagaggc tgaacgccga gaagatcaaa
     4201 accacgggga gaataaacag ataattgccg caataatctt tcagccggtt ccaggttttg
     4261 gattccgtta agtgctgtgc cacgcccttc acccttttct gtatagcctg ccggggggcg
     4321 gttaggcccc ccggcaggat caaagataga ctacttcaac agcgaagccg cccaaatttt
     4381 attcacatca tccaccactt gttgcggggt cttcttcttc agcatgatgg attgaattcc
     4441 ccgggtgatc tcggtgtcca catcaccgcc gcggcccttc aaaccgacgt taggaatgat
     4501 gcgaccggcg tcatcggcaa aatcacccac gccaccgtcg agtttgactt tcttggtaac
     4561 ttcggagctg gccggcaggt taaagctggt cttggcgtat tgggcttgtt gctccggagc
     4621 ggtcaaccac ttcaagaagt tgatggcttc atccttattc gggctcactg cgttaaccgc
     4681 caaaactaca ccgtaaccgc cttggatgta ttgaggatat ctgccggcag aaggcggtaa
     4741 aaatgcgccg accttcggta ccaaattggg attggccgat ttgatgacgc cgatacccca
     4801 agacccgtca tataacatgg cgactttctc ctgggcgaag agacgttccg cgtcaggatt
     4861 atcccatgtg gccatgccgt cggcatacat tccggcatcg cgcatatcgg cgaagatctg
     4921 gaaggttttg acccagttct tgtcggtgaa gcgtttcccg ttattttggg cctgaatcat
     4981 gcccttctcg cccatgatgt tccaagcgta tacttcccag atgctaatcc cgagccaggt
     5041 cccgaatcca cccgcaaaag gagtgatccc ggcggccttc agcttcttgc cggcagcgat
     5101 gaactgagcc caggttttgg ggggacggtt cggatccaaa ccggcttttt ggaagagttc
     5161 cttattgtac aggatctgca tattgaccga atctaacggc aggccgtaca aaccaggctc
     5221 tacgccgtat tggttgccgg aggcaaagga gatattggaa agaagggaag gaatgtaatt
     5281 cttccgccag gcaccggtta cgtatttatc caaattgagc attccgccgg ctttaatcca
     5341 acgggccttg ccttcgatct taaccgggtc ggcgctaccg ttactggtat aaatgtccgg
     5401 tgcggtaccc gcctgaatcg cggcttccac tttcttctcc acatcctggt caccggggaa
     5461 gagctggaca tcaactttca ccccggtagc cttctcatac tgctgggcaa agctttgcag
     5521 cagatcgttc cggtccacca tataatgcca gaaagtaatc gtcttgggcg ccgcggaggc
     5581 gaatggaacc gctaaaatcg cggtcagcac tactgccacc aataaaaccg ttaataacga
     5641 ctttttcaag agatactcct cctttaaata gatattgaaa gaaaaaaacc ggataaaagc
     5701 cgcaattcct ctccttaacc accccccgcc aatattggat aattttttga atgaccgata
     5761 attttggaac cgcttccaac acaataataa ttaaacccga tcgattgata tccttcttaa
     5821 gtttccgaaa tttctcagaa aattttttcg aaaaaataag gctcaaagtt cgatgtgcct
     5881 ataaatatag gtatatcgtt actttcagcc ttcttatctt gcatcagctt tatggaatcg
     5941 gttcaattta ccttatcaag atttgacact tagccaataa tccgtcctat ggtttgcaac
     6001 gcctttaatt cagcccagcc gtaacaataa aagaaaaatc catcggcatc gcctctgcgt
     6061 gcggaatcgc atgccttatc gatctcttcc attggcgtat tccatatttg aatgccgcta
     6121 aaaatctgtt tgcagtggcc aaaagcctgc ttggcaatac ctgtttcccg gccgacaaaa
     6181 tctacgggaa atccaaattc tttaaacttt tcaaaactca tggcataagg gatctgcaaa
     6241 agttccatga ccataagaaa gattttttcc tgggcttccc tgtcttcatg aatggcgagc
     6301 gcctctacca gtcccttcaa atctgcgcca ccgcccaatc ggtgataagg aaaatgtttc
     6361 gccccgtcgc ataaccttcc taatgcttga taatcttggc cgagaaacca agcaaacgaa
     6421 ggcggccata aattaagcca aaatttcgta ttcatatgcc tggtttgatt atatattttc
     6481 acctgggtgc tcactcgatc aaccaatcgc gtaattaaag cggccctgaa cccaagccat
     6541 tttctaatct cattcaattc agcgccggaa agattctgaa accgcccccc cggttgatcg
     6601 ggctgtttta aaaacctttt gagaacactt tttaataacg ccaagtcgat tccttcatct
     6661 tccatcatcc ggcagcacga atcacaaaaa caggttagca tcctctcggg ctgaacatta
     6721 gctccgctcc aatcggggta acgcatgcga tccagcagga tcgcttcgga aggatatcga
     6781 tccagaattc cttcaatcaa cgaggcaata tattcgaaag tctccgggcg ggatgggcac
     6841 aaccaggtcg gaaccggttg accatccagt gttttgaccg acaaatcctc aattcgaccg
     6901 gaaaaagcgc cgtttagtcc tttgacccag gggatgacag tagccccacc aactgcggct
     6961 gcttttctca gcaacccgat atagtcatta ccagccaaaa cctcagccga aggcaccggc
     7021 tgaattgata accccgaaaa aaggcggccg gataaaggaa cttcaagagt ggcccagctg
     7081 actaccactg aatgccgggg gttatgaggc aagaccccat cggggtaggg atgcctttct
     7141 tcaagtgtca tcgcttctgc aataatggtc ttaatttttc caagttccgt gatgttttcc
     7201 accacacgct cggcaccctc atcccgaata tcatggggat ggacatgaat cgcgtataac
     7261 attccttatc tagcgggata caagccatgt gtatcattaa atcataaacg tctttacacc
     7321 gcttttaata ctcggcggta actcccgcta aaacctcctc actggatgga ttaccggtta
     7381 gctccttaac catccggcct ttacgcataa tcaaaaccct actgcagatc cgggagagct
     7441 cttccgggtc atcggagatt acgatcaatc cactgccgtc atcggccaat tctctcagta
     7501 ggcggtagat ctcttccttg gtgccgatat cgattcccgc ggtaggttgg tcgaggatca
     7561 gaatatgcgt cttggctgcc aaccacttag ccaatacgac tttttgttga tttccgccgc
     7621 ttaaagtacc ggctagtact ttggaatcgg tacatttgat acttaaccgt tgaatcattt
     7681 tttccatcaa ttgacgacgg gctgatagat ccataaacca accgtggcgt aacctactgg
     7741 tactggagag taaaatgtta tcttccactg gacgaattaa aaccagaccc tgctgtttcc
     7801 ggtcctccgg agccaagccg ataccgcggg agatagctgc ctttggggat gtagcctgaa
     7861 atatcgagcc gtttacatcg attcggcctc ggatcttttt gcgaggttcc gcgccgaaga
     7921 ttgtacgcgc cagctcggtg cggcctgaac ccaccagacc ggcaatacct aaaatctcac
     7981 ccgcatataa tgaaaaactg acatcttgaa atttggcgct attacttaag tgctcgacat
     8041 ttagaatggc ctgatccttg tgagtatccc gtttactatt atccatcata acttcatggc
     8101 cgagcatcat cttgatcaac tcaggacggt ttagctcaga aatcaaagca gtacccgcat
     8161 ttcggccgtc ccgcataacc gtaacccgtt cggcaatcat aaaaacttct tcaagaaaat
     8221 gggatatata aacaatactg gtcccgccca cttttaaacg gcgcataatc tcaaacaggt
     8281 tatcccgctc agtacgggaa agccgtgcag taggctcatc catcaccaat acgcgagcgt
     8341 gagtagccat cgcttttgcg atctgcacca actgttgatc cgcgactccc agttccgcca
     8401 cttgaacatc caagggtaac gtaaatttta actcggccaa caactccgcc gcttcatcat
     8461 gcaacgccgg ccagtttagc aaaggaatcc ggcccaattt ccgtaacggc tcacggccta
     8521 acagaatgtt ctccgccacc gttaattgcg ggactagatt gaactcctgg tagatcacgg
     8581 cgatcccgga ctccaaagcc agccggggat tactgaagcg aatttcctta ccatgcaata
     8641 tgatggttcc ttcataatca gggtaaatcc cggccaatac cttcatcaac gttgacttgc
     8701 ccgcaccgtt ggcccccacg agggcgtgga tttcaccagg ttttaggaga aaattgacct
     8761 tatcaagcgc atggacaccg gcaaattttt ttacgatatc ccgcatttcc aatatgaaat
     8821 ctgtgtccat ttccatcatt attggcccct ttatgttttt taatatccgg gaggattcca
     8881 aatcctcccg gatattcacc gggattctta ccaagccgga ttatacttcg aaacattgtc
     8941 cttggtaata agcggtaatt ccaaaaagta attgggggtc ggaatttgcg acttctttcc
     9001 ggtcagataa agaagtgaca tatgcatccc ttcaacagct tgaggataag gatcttgatc
     9061 cacagttccg tatacattgc cattttgaat ggcttcgcgc acatcttgcg gataatcgcc
     9121 caagatccag cggacctcgt cccgtcccga atcatgagta aatttagcgg cggctacagc
     9181 ttccggtccc tgacaaacaa tcgcatctat ggaacctttg gcatagcgcg agagcatatc
     9241 ctgggctgca gctaaggctt tggcgctatc ccaattgtca ctgatctctg agaccacttt
     9301 gatattgggg taatccttta aaaagtccgt aaatcctttt ttccgtaaag tctgtgcgga
     9361 ggtacctaat tgccccatga tataaccgac ttgaccggaa ttgccaatcg ctttagtcaa
     9421 cagcttggcc tgctgttttc caaaatagta atcatcggcg cctacaaagg taacaatctt
     9481 tgcgcctgca ccggcctggt tattaaccga aatcactgga attttagcag cattcgcctg
     9541 ttgaataact ggaaccacag cctgagcgtc tccagggacg attagaatca tgtcgacttt
     9601 ttcggcaatg aactggcgca cgactgccac ctgcgtattg ggatcacctt gtccatcgcg
     9661 gagtaaaagc tttaaaccat acttagcggc accgtcatgc aagcctttaa tgaaattgga
     9721 ataaaacgga acggacatgt tccaaatcgt tgcgccgaca gtgtattgct tggcggcatt
     9781 aatgttgatg cttacggaaa acaccatagc caaaagggcg acaaacaaaa tactcttctt
     9841 gatgcgcttc attaattttc ctcctggttt ttttagactt attcccattc acgaaaaaag
     9901 taattcctaa gccatcacct ccttcaatct cgttgtcaag ttctcatttg gttcaacggc
     9961 gtttccccgc agtatccaga cttacagcaa taagaataat caagcccgtc accgcaggtt
    10021 gccaataagg tgaaacattt aacaaattca aagcattggc aatcatccca agcaagagaa
    10081 caccgagaac tgttttcata atactgcctt ctccgccggt cgtcgaggtt ccaccgacaa
    10141 acgtagcggc aattgc
//
LOCUS       DASVMB010000047         8994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_372657,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000047 DASVMB010000000
VERSION     DASVMB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372657"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VF531_01610"
     CDS             <1..1549
                     /locus_tag="VF531_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX7712692.1"
                     /translation="SIGSCRGASSHQHNPFLALLQKDANEDYGEVFAFNLVYSGNFLA
                     QVEVDQFRTIRVGMGINPFDFSWLLEPGESFQTPEAVMVFSSKGLGAMSRTFHQLYRT
                     RLCRGQYRDQVRPILINNWEATYFDFNADSIERLAKTAQDLGMELFVLDDGWFGKRND
                     DTSSLGDWFVNREKLPLGLEDLANRVRKLNLRFGLWFEPEMISPDSELYREHPDWCLH
                     VPGRNSSEGRHQLILDLSREDVCRALITMVSDILRSAPITYVKWDMNRHMTEIGSANL
                     PPERQRETAHRYMINLYRIMDYITTAFPDILFEGCSGGGGRFDPGILYYMPQIWTSDN
                     TDAIARLKIQYGNSLVYPAITMTSHVSAVPNHQLKRVTPLDTRGHAACSGNLGYELDL
                     NKLGPEEQMLVKEQVAFYKEIRPVVQFGELYRLQSPFEGNEAAWMYVSEDKKEAVAFY
                     FRVLAEAAPPLRTLRLKGLDEQLDYKIHSEDRIYSGDVLINAGLNIPETKMSGDFQSV
                     CWRLKAV"
     gene            1864..3066
                     /locus_tag="VF531_01615"
     CDS             1864..3066
                     /locus_tag="VF531_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX7712693.1"
                     /translation="MGKTLIIGAGGVSSVVVHKCCQSPEVFEDICLASRTLQKCDAIK
                     RKLAGSRTRIHTAQLDADKTDRVIDLIKSYKPDIVINVALPYQDLSIMDACLATGVDY
                     VDTANYEPPDIPKFEYKWQWAYKEKFTKAGITALLGSGFDPGVTGVFCAYAQKHYFDE
                     IHSVDIVDANAGDHGYPFATNFNPEVNIREVTANGRYFESGSWIETEPLAVKKVYNLP
                     EIGPKNIYLLYHEELESLAVNIKGLKRIRFWMTFSESYLNHLRALQDVGMTSIQPIEY
                     EGKQIIPLQFLKAVLPDPASLGPRTKGKTNIGCIIQGIKNGKPKTYYVYNVCDHEKCY
                     AEVGSQAVAYTAGVPAMIGAMMVLKGFWKKPGVFNVEEFDPDPYMDALNRYGLPWQED
                     FSPSLIEG"
     gene            3178..4317
                     /gene="nspC"
                     /locus_tag="VF531_01620"
     CDS             3178..4317
                     /gene="nspC"
                     /locus_tag="VF531_01620"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEX7712694.1"
                     /translation="MNLDFNALPSPCYVVDERLLIKNLEILRSVEERSGCGILLALKG
                     FSMFAVFPLVAQYLKGVTASSLFEARLGFEQMGKEVHVYAPAYVPGEFDELLRYCDHI
                     VFNSFSQWNRFKAKVQNAAAKKIECGIRINPEYSEIEVPIYNPCYQNSRMGVTLANFR
                     PDELDGIDGLHFHTMCEQNSDTLERTIKVVDEKFGPFIKGMKWLNFGGGHHITRSDYD
                     IDTLIRSIMFFKDKYGVDVYLEPGEAVALNTGFLVATVLDVVENGMKTAILDTSAACH
                     MPDVLEMPYRPNIIDAGKPDEYPYTYRLGGRTCLSGDIIGDYSFKKPLEPGDRLVFCD
                     MAHYSMVKNNTFNGLNLPAIALFNETEGIKIVRRFGYEDYKSRLS"
     gene            4931..6076
                     /locus_tag="VF531_01625"
     CDS             4931..6076
                     /locus_tag="VF531_01625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEX7712695.1"
                     /translation="MSKKILIIIVAILLLSVIGIFVLFRSGPGQMDPKNLGINPENLP
                     KVVTDEDLKFHTELERIYGFIGKDVSEKDLSGVSIDCLSNISFDQNTKWPAENKLPQG
                     FNPDIWMNTGRDPGLNIQKIHERGINGKGITVAIIDKPILPTHNEFDDRMTYVKVGTP
                     QTNPHFHGLACASILAGKTCGVASGAKIYYFATPDVGDNFKYYIDAINSIRRINKSLP
                     SNEGIKIVSISDGIGEYDRNWSKWQETLKKAETEGLVVVYSNLLRGVTWGGCIPSSDG
                     NNPRNYKLSNYLKGSTFVSTFILVPGDFRTTASNNGDEQYIYWGEGGFSWAIPYVVGL
                     AALAWQVNPDLSFDKIMERLIETKTTTAEGRFIIDPEAFIRSVGSRR"
     gene            complement(6216..6323)
                     /locus_tag="VF531_01630"
     CDS             complement(6216..6323)
                     /locus_tag="VF531_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX7712696.1"
                     /translation="MLFDDPRLFFIHFESIDEPLSFAKKVAKTFKLLKC"
     gene            complement(6447..7115)
                     /locus_tag="VF531_01635"
     CDS             complement(6447..7115)
                     /locus_tag="VF531_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7712697.1"
                     /translation="MWTSKEGDVRREEFLKTAFELFLEKGYEKTSINDIIKKIGVTKG
                     AFYYYFKSKDEILNVLCMEQAEHFLNIVQQISADGKLSALDKINQIITNVFRYRAAQM
                     ENRLKFLKLLENEGNLKLAGRINEYILLYGSPVIRSIIEQGIHEGTFVTEFPAEAAEL
                     YIQLSNIMNNVITKLLMNTQNRSEAEAAIKKKTLFYEETFEKLLGIKKGCIHLSDTIL
                     QYYF"
     gene            7474..8469
                     /locus_tag="VF531_01640"
     CDS             7474..8469
                     /locus_tag="VF531_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7712698.1"
                     /translation="MNNKKHEITIAAIQMESFHGMIRENQIRATKLIEEAANRGAELI
                     VLPELFSCGYISNPDIWRYGESQDSPTIQWLVETSKRLGIYLGAGLVEIIGNDFVNEF
                     VLANPKGEIEGRAQKKNAEAYCFRRGKGVHIIHSSLAKIGVGICADNHFSDFIGHMRE
                     NDVDILLMPHAWPSPSTVSKIVSTEDIQKEEEEIKTFASLITSLLGVPSVFVNQIGKI
                     APMAGIFGGVMNPDSFRLHGFSRIVDAPGRTKAELMNQEGIAIATVTLDPSCKKNIAI
                     PNFDGWIHPGSTIVRKIIIPIDTLFGSIYYRLSVKRKIEAKRRRVVSSFYAKGSL"
     gene            complement(8574..>8994)
                     /locus_tag="VF531_01645"
     CDS             complement(8574..>8994)
                     /locus_tag="VF531_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7712699.1"
                     /translation="MVAFAIPAGIIATRIGKKKTIFIGLVLIILLFGGLFYSTDINTI
                     GYLFIIAGISATLIHINSYPLVVSMTTSSNIGRFTGYYYLASSLANIVSPPLAGGLMD
                     IFGYGILFKYAVVGFILALVCLLMVKTPSEKPLVTEL"
BASE COUNT         2567 a         1778 c         2141 g         2508 t
ORIGIN      
        1 atccatcgga agttgtcgtg gcgccagtag tcatcagcat aatccgtttt tggccttact
       61 tcaaaaagat gcgaatgagg actacggaga ggtttttgct tttaatctgg tttatagcgg
      121 caacttcctg gcccaggttg aggtggatca attccgtact atacgagtgg gaatgggcat
      181 caatcccttt gatttttcct ggctgttaga gccgggcgag tcatttcaga cccctgaagc
      241 ggttatggtt ttttcctcta agggtttagg ggccatgtcc cgcactttcc atcagttgta
      301 tcggaccagg ctgtgtcgcg gccagtatcg ggatcaagtg cgcccgattt taatcaacaa
      361 ttgggaagcg acttatttcg attttaatgc cgacagcatt gaacggcttg ctaaaaccgc
      421 gcaggattta ggaatggaac tgtttgtact cgatgacgga tggttcggga aacggaatga
      481 cgacacgagt tctttgggag actggttcgt aaatcgcgaa aagttgcctt tagggttgga
      541 ggatttggca aacagggtcc gtaaactaaa cctccgattc gggctgtggt ttgaaccgga
      601 aatgatctcc ccggatagcg agttgtaccg ggaacacccg gattggtgcc tccatgtgcc
      661 gggtagaaac agttccgagg gacggcatca attgattctt gatttatcac gtgaggatgt
      721 ctgccgggca ctgattacga tggtatccga tattttaagg agtgcgccta tcacctatgt
      781 caaatgggat atgaaccggc atatgacgga gattgggtcg gccaatcttc ccccggagcg
      841 ccaacgggaa acggctcatc ggtatatgat aaatctttat cggatcatgg attatatcac
      901 cactgctttc ccggatatcc tgtttgaagg ctgttccggg ggtggcggtc gttttgaccc
      961 cggtattctc tactatatgc cccagatttg gaccagcgat aataccgacg cgatagcccg
     1021 gctaaaaatc cagtacggga acagtttggt atatccggca atcacgatga cctcccatgt
     1081 gtcggcggtt cccaatcatc aattaaaacg ggtcactccc ctggataccc ggggacatgc
     1141 ggcatgttcc ggtaatttgg gatatgaact tgatctgaat aagcttggcc ctgaagagca
     1201 aatgctcgtc aaggaacaag tggccttcta caaagaaatt cggccggtgg tgcaatttgg
     1261 tgagttgtac cgcttacaga gccccttcga aggaaatgag gccgcttgga tgtatgtatc
     1321 cgaagacaaa aaagaagctg tggccttcta tttccgagta ttggcggaag cggctccgcc
     1381 tctcagaacg ctccgtttga aaggcctaga tgagcagttg gattataaaa tacactccga
     1441 ggaccggatc tatagtggcg atgtacttat caatgccggg ctaaatatac ccgaaacgaa
     1501 aatgtccggg gattttcaga gtgtctgttg gcggctcaag gcggtgtgat tggaacgtgt
     1561 gaaatcgata cccggatgcg cgatcaaaca aaaggaacga tagttcatgc ggaaggtcac
     1621 aacgatctac agtcattgca agcacacgtt ccataaaatt atgaaaattg taaaacaatg
     1681 caatagaata attcatttgg aagatgtttc tacaaacctg gtcctttttt agaaaagggc
     1741 ttcttttttt gtcattttta ttgattgttt ggaacatttt ctgtataatc atcctggtaa
     1801 aaaggtcccg ttgcaatcat cctggttaaa cgaatttatg gaaattagga ggggtttacg
     1861 gatatgggaa aaactttgat tatcggcgct ggaggagttt ccagtgttgt ggttcataaa
     1921 tgctgccaga gtccggaagt ctttgaggac atttgtttag ccagcagaac cctgcaaaaa
     1981 tgcgacgcca tcaagcgtaa attggccgga agccgtacca ggattcatac tgctcagctt
     2041 gatgccgata agaccgacag ggtcatcgac ctgatcaaaa gctacaaacc ggatattgtg
     2101 atcaatgtcg ctcttcctta tcaagatcta tccatcatgg atgcctgctt ggcgacaggc
     2161 gtcgattacg tggataccgc caattacgaa cctccagata ttcccaagtt cgaatataag
     2221 tggcagtggg cgtataagga aaagttcacc aaggcaggaa taaccgcgct gttagggagc
     2281 ggatttgatc ccggtgtcac cggggttttt tgcgcctatg ctcaaaaaca ttattttgac
     2341 gagattcatt cagtggatat tgtcgatgcc aatgcgggcg atcacggata tccatttgca
     2401 accaatttca accccgaggt caatatcagg gaagtaacgg caaatggccg ctattttgag
     2461 tccgggtcct ggatcgagac tgaacccctt gccgtgaaaa aagtatataa tcttccggag
     2521 atcggcccga aaaacatcta tctgctatac catgaggaat tggagtccct ggccgttaac
     2581 atcaagggac tgaaacggat acggttttgg atgacttttt ccgaaagtta tctcaatcat
     2641 ttacgtgccc ttcaggatgt ggggatgacc tccattcaac cgattgagta tgaaggaaaa
     2701 cagatcatcc ctctgcaatt cctgaaggcg gttttaccgg accccgcatc ccttgggcca
     2761 agaaccaaag gtaaaaccaa catcggttgt atcatccagg gaatcaagaa cggtaaacca
     2821 aagacttatt acgtttataa tgtctgtgat catgagaaat gctatgccga agtcggttcg
     2881 caagctgttg cttatacggc cggcgttccg gcaatgatcg gcgccatgat ggttttaaaa
     2941 ggcttctgga aaaaacccgg cgttttcaat gtggaggaat tcgatcccga tccctatatg
     3001 gatgctttga atcgatatgg attgccgtgg caggaggatt tttcgccctc cctcatcgag
     3061 gggtaagttg ggggtccata cttttttgca taggaaggtt ccaacagaaa cccgcgtcta
     3121 gtcgtatgta atcgctggat gtatgcagca aagtaagcta cggaatggag atcgaaaatg
     3181 aaccttgatt ttaacgcgtt accgtcaccc tgctatgttg ttgatgaaag gcttctcatc
     3241 aaaaatctgg agatactccg ctcggtggag gaacgttcgg gatgcggtat tttgctagcc
     3301 ttaaaagggt tttcaatgtt cgccgtattc cctttggtgg cccaatatct aaagggcgta
     3361 accgcgagtt cgttatttga agccaggttg ggttttgagc aaatgggcaa ggaggttcat
     3421 gtttacgctc cggcatatgt ccccggagag tttgatgaac tccttcgtta ttgcgatcat
     3481 attgtcttca attcttttag ccagtggaac cgcttcaagg cgaaggtaca aaatgcggct
     3541 gccaagaaga ttgagtgcgg gattcgcatc aatcccgagt attccgagat cgaggttccc
     3601 atttataatc cttgctatca gaactccaga atgggagtga ccttggccaa ctttcgcccg
     3661 gatgagcttg acggcattga cgggcttcat ttccatacca tgtgcgagca aaactcggat
     3721 accctcgaac gcaccatcaa ggtggttgat gagaagttcg gccccttcat caaagggatg
     3781 aaatggctca atttcggcgg ggggcatcac attacccggt cggattacga tattgatact
     3841 ctgatccgtt cgattatgtt ttttaaggac aagtatggag tagatgtcta cctggaaccg
     3901 ggcgaggcgg tggctttaaa cacgggtttc ctggttgcca cggtcctcga tgtcgtggaa
     3961 aacggcatga aaaccgcaat cctggatacc tcggccgcct gtcacatgcc cgatgtgctg
     4021 gagatgcctt accggccgaa tatcattgat gccggaaagc ccgatgaata tccttacact
     4081 tacagacttg gcggcaggac atgcctttcc ggagacatca tcggcgacta ttcgtttaaa
     4141 aagccgctgg agcccggaga tagactggtc ttctgtgata tggcccatta ctccatggtt
     4201 aagaataata cattcaacgg cctcaatctc ccggccatcg ccctttttaa cgaaacggaa
     4261 gggataaaga tcgtgagacg tttcggttac gaagattata aatcccggtt gtcataaaaa
     4321 aaatttgatc aaatcgcttc aatcagtttt catatactca aacgcccttg caggggcgtt
     4381 ttttgtttct ttaccaaggg aatatcgaag ggttgttctc gaactctaac ccaagccttt
     4441 gtctgcccaa cgaaactaaa ttttacgggt gagtcagtaa aagcaagtaa gagaaaataa
     4501 gctcagatgg cttgtaaagc gaacgcgttt ggctaattat ttgaaaagac ttcactggta
     4561 aagtgccttt ctagggtttg taaataagtc gtccgtttta tgccgtgttg gatatgagta
     4621 ggaaaagaat agatatatga tttgcctggc aaccagtatc ataaattgcg atggttattt
     4681 ttatcagata aggaaggatt tctataaatt ttacagaatt ttatgtcggt attgtggctg
     4741 taagatgttg gggtttttaa gtttttttag acagggagga agaatcaaca tgaaaaattt
     4801 taggccaatt ccagcggaca gttactagag gattttaagg aataaaaatg ttgattattt
     4861 aagggacgag aactgttaag gtttatcagt ctttattttc gatgaatcga gttgaagcgg
     4921 ggggtaaaaa gtgtcaaaga agatcttgat tattattgtc gcaattttac ttttgtcggt
     4981 aataggaatt tttgttcttt ttcgatcggg tccaggccaa atggatccga agaacttggg
     5041 gatcaatccg gagaaccttc ccaaagtggt cacggatgaa gatctgaagt tccacacgga
     5101 attagaaaga atatatggat ttataggcaa ggatgtatcg gaaaaggatc tatcgggggt
     5161 ttcgatcgac tgcctttcaa atatcagttt tgaccagaat acaaaatggc cggcagagaa
     5221 taaactaccc caagggttta atccggatat atggatgaat acagggagag atccgggatt
     5281 aaatatacag aaaattcatg aacgagggat taacggcaaa ggaattactg ttgcgatcat
     5341 cgataaaccg attcttccta cccataacga atttgatgat cgaatgactt atgtcaaagt
     5401 aggaacgccc caaacaaatc cgcattttca cggtttagca tgtgcctcta ttttggcggg
     5461 taagacctgt ggggtcgcga gtggggcgaa aatatactat tttgccactc cggatgttgg
     5521 agataacttc aagtattaca tagatgcaat taacagtatt cgtcggatca ataaatccct
     5581 tccttccaac gaaggaataa aaattgtctc tatctccgat gggataggag aatacgatcg
     5641 caattggagc aaatggcaag aaactctaaa aaaagccgaa acagaaggac tggtagtggt
     5701 ttattccaat ttactacgag gggttacatg gggcggatgc attccctcaa gtgacggaaa
     5761 taatccacga aattataaac tcagcaacta tctaaagggg agtacttttg ttagtacttt
     5821 cattcttgtt ccgggagatt ttcgaaccac agcaagtaat aatggagacg agcaatatat
     5881 ttattggggt gaaggaggct ttagttgggc aattccttat gttgttggac tggccgcttt
     5941 agcatggcag gttaatcccg atctcagttt cgacaagata atggagagat taattgaaac
     6001 aaaaactacg actgccgagg gaagattcat tattgatccg gaagcattca ttcgttcagt
     6061 aggttctcgc cggtagtaaa tattccaagc aatgttacat aatacgactt taaaagagta
     6121 agtgactatt gaaataagac aaatttccat tagtgtagca agaaaacggt agctgccggt
     6181 tgcggtaact accgttttac actagaaaag tgagattagc acttcagtag tttaaatgtt
     6241 ttggctactt ttttcgcaaa cgataacggt tcatcgatgg attcgaaatg aataaagaac
     6301 agacgaggat catcgaataa cagtgattat gcaatgccgt taccagaatt aagtgtcatc
     6361 atatgaggaa ctgaatgggt tgttgataat atcttcgcct agtattacat ttgtagtaac
     6421 aaaaaggatt acatcaataa tagtttttag aagtaatact gcaaaatcgt atctgataaa
     6481 tgaatacatc cttttttgat acccagaagt ttctcaaagg tttcttcata aaatagtgtc
     6541 ttttttttga tggcggcttc ggcttcggaa cggttttgag tattcatcag aagttttgta
     6601 atgacgttat tcatgatgtt acttaattgt atatatagtt cggcagcttc ggcaggaaac
     6661 tctgtaacaa acgtaccttc atggataccc tgctcgataa ttgaacgaat caccgggctt
     6721 ccgtagagca ggatgtattc attgatcctg ccggcgagct ttagattacc ctcattttct
     6781 aagagtttta aaaatttgag ccggttttcc atctgggctg ccctatatct aaatacattc
     6841 gtaataattt ggttgatttt atcaagggcg cttaatttgc cgtctgctga aatttgttga
     6901 acaatgttta gaaaatgttc tgcctgttcc atacataata cattcagtat ttcatccttt
     6961 gacttgaagt aataataaaa tgcgcccttg gtgaccccaa tttttttgat gatatcgttt
     7021 atcgaggtct tttcatatcc tttctccaga aacaactcaa aagccgtctt tagaaattcc
     7081 tctctcctca catcgccctc tttacttgtc cacataaaaa ttcctccgtt aggttagcct
     7141 gtcggaataa ccaagattgt gtcggtatta tcttatataa acgggtgtct ttctgtcaac
     7201 ttttgcaacc gcttgctgcc ggcattgacg tcaagaccag tgaaaatatg gtcaagcggg
     7261 ttaagatatc acaattcttt attgattgac aatatggcga aattggaata aaatagtaca
     7321 taccaatggt acgtacatac cattggtatg tacgctatcg aatcctatat gagcgattcc
     7381 attccgggag gctgaataat gacaaatgaa acttaccaat ggcaaaaggc ttgaaaacat
     7441 taacgaacct tgatacttat gggaggtata caaatgaata ataaaaaaca tgaaataacc
     7501 attgctgcta ttcaaatgga atcttttcac ggcatgataa gggagaacca aatacgcgcc
     7561 acaaaattaa ttgaggaagc cgcaaatcga ggggcagaac tcattgtgtt gccggagttg
     7621 ttttcttgcg ggtacatctc caatccagac atatggagat atggtgaaag tcaagattcg
     7681 cctacaattc aatggctggt tgaaacatca aagaggctgg gtatctattt gggcgcgggt
     7741 ttggtggaaa taatcggcaa tgattttgta aatgaatttg tgcttgcaaa tcccaaaggt
     7801 gaaatcgaag gacgggccca aaagaagaat gcggaagcat attgttttcg gagggggaaa
     7861 ggggtacata ttatccattc atcccttgca aaaataggtg ttggtatctg cgcagataac
     7921 catttttcag attttatcgg gcacatgcgg gaaaacgatg tggatattct attgatgcct
     7981 catgcatggc cttcgccgtc tacagtatcg aaaatcgtca gtactgaaga tattcaaaaa
     8041 gaggaagaag agattaagac atttgcttcc ttgattacaa gtttacttgg tgttccttct
     8101 gtctttgtaa accaaattgg caaaatcgcg cctatggccg gtattttcgg gggagtgatg
     8161 aatcctgaca gctttaggtt acatggcttt tccagaattg tcgatgcacc cggtagaaca
     8221 aaggcggaat tgatgaatca agaaggtata gctatagcta cggttactct tgacccttca
     8281 tgcaaaaaaa atattgcgat cccgaatttt gatggttgga ttcatccggg atcaacaatc
     8341 gtaagaaaaa ttatcattcc catagataca ttatttggat caatatatta caggttaagc
     8401 gtgaagagaa agatagaagc aaaaaggaga cgagttgttt catcctttta tgcaaaggga
     8461 tcattgtgat cgagaagggt taccatggtt tggaaaatga attaattatg aatgtccaaa
     8521 ggtaaccgga aaaatcgatc gaatcggatg cggggaggtt ggacctcccc gacctataac
     8581 tccgttacaa gcggtttttc acttggggtc tttaccataa gcagacagac gagcgccaaa
     8641 atgaagccta ccactgcgta tttaaagagt atcccgtatc cgaatatatc cattaacccg
     8701 cccgcaaggg gaggagatac gatatttgcc agagatgagg ccaaataata atacccggta
     8761 aatctcccta tatttgagct ggtcgtcatg ctgacgacca acggatagga attgatatgg
     8821 atgagggttg ccgaaatgcc tgcaatgata aataaatacc cgatggtgtt gatatcggtc
     8881 gaatagaaca gccctccaaa taaaaggatg atcaagacca atccgatgaa gatcgttttt
     8941 ttcttgccga tccgggtggc gattatgccc gccggtattg caaaagccac catt
//
LOCUS       DASVMB010000048        14140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_667764,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000048 DASVMB010000000
VERSION     DASVMB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667764"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VF531_01650"
     CDS             <1..823
                     /locus_tag="VF531_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7712700.1"
                     /translation="AWFLIISGVVWGGIQGLFMNYNLQTIALLWFLLPLFFLPGMLRV
                     VNQYERGVLFRFGRQVGVLDPGMNIIFPMGIDFVRIVDLRTFTIDVPKQEVITRDNVT
                     INVDAVVYFNVFDPVLAIIKVADHVRSTSLLAQTILRSVLGQHELDEMLAKRTELNEI
                     LKKLLDEATDPWGVKVTAVEIKAVELAESVKRAIARQAEAERERRAKVIAADGELQSS
                     EKLSEAARVLATQPASIQLRYLQTLTEIAVEKNSTILFPIPIDLFQGMLPKGKQE"
     gene            944..1204
                     /locus_tag="VF531_01655"
     CDS             944..1204
                     /locus_tag="VF531_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015274273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7712701.1"
                     /translation="MPTYEYVCPKCGVFEEFHSITTQLTRCPKCNSEVRRLISQNTNV
                     IFKGSGFYTTDYRSGSHSSSSGTSSPKTDTASPSSKPESKAS"
     gene            1369..3051
                     /gene="argS"
                     /locus_tag="VF531_01660"
     CDS             1369..3051
                     /gene="argS"
                     /locus_tag="VF531_01660"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7712702.1"
                     /translation="MEQIVTRRVLQKLRESLTTAAEQARSQGRLVFEELPEFTLEVPK
                     DKNHGNFATNLAMMLSKPNRKPPRELAELIIANLPSNPYCDRVEVAGAGFINFYLNNT
                     WLLEVAESILTEKERFGNNADLTGFKVQLEFVSANPVGPMNVVNARAAALGDTLGNLF
                     TACGAEVEREYYVNDFGVQVTALGRSLDARYRELLGQTVEFPEDGYQGEYIYDMAREL
                     IDAQGDSYLKLSETERIQTFRDLGYRRILKTQKTDLENYGVHYDCWFSERSLHEGGEV
                     AKVLQTLTDRGLTYEQDGALWLATSKYGDDKDRVLRTADGRTTYFLSDIAYHLNKFGR
                     GFERVIDIWGPDHHGYIPRMKAAMAAMGIEPDRLEVLIAQQINLLKEGKPFKMSKRRG
                     EFITMTDLLQEVGNDGARWFFLMRSSDSHLDFDLGLAKSQSNENPVFYVQYAHARICS
                     IFKQSAGGSLPGPEDIPGLSRHWDEEEQALLEKAALFPELVVGAAHHREPHRLTGYLL
                     ELATLFHSYYNRRRIIAEEPEVTRARLAVAESLGYIFRRGLELLGVSAPEHM"
     gene            3178..3930
                     /locus_tag="VF531_01665"
     CDS             3178..3930
                     /locus_tag="VF531_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712703.1"
                     /translation="MRKLPLFPTLGDSLKKTFKELYSSMGFSFLLSLLWFTAYLPIIF
                     VFISIYFNPTLLSKVKQPGDIFVLFFGMSLVCTIWNGLVAGPVTTALYGIYENRKGDY
                     PSTRMFFQIFKKCYTRSAGVHFVYSLVVTALLLNFVIIFTRPNVLMLIAGIISLYMLF
                     FLALLQFYFHPLLFLDNGFKKVVKKSFLLTVDNLGLSFWYAVIIGFLPVLTLLIPQIM
                     PVTMLVLFFIYGAFIIYIINHGFETIYAKYDE"
     gene            3967..5574
                     /locus_tag="VF531_01670"
     CDS             3967..5574
                     /locus_tag="VF531_01670"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7712704.1"
                     /translation="MAKFIFITGGVVSSLGKGITAASLGCLLKSRGINVSILKLDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDVNLSKNNNVTTGKIYWSVITKE
                     RKGDYLGGTVQVIPHITNEIKDRIHRVAKESQADVVLVEVGGTVGDIESLPFLEAIRQ
                     FKGDIGRDDVMYIHVTLMPFIGAASELKTKPTQHSVKELRSIGIQPDVIVCRSEKPIS
                     NELKAKIAQFCDVNKEAVIPNLDAKTIYEVPLLLEQAGLDQIVIERLKLLCKERDLQV
                     WREMVSKMKSLKGKVTIAIVGKYVSLPDAYLSVAEALRHGGIVHDTQVQIKWVNSEVI
                     ERDGVEKVIGQVDGILVPGGFGNRGIEGKIEAVRWAREQKIPYLGICLGMQCAVIEYS
                     RNVCHLEKANSSEFDPDSPHCVVDLLPEQKEIEDMGGTMRLGLDPVQISQETLAHKAY
                     QQELIYERHRHRYEVNNEYLSKLQQAGLLISGFLPDRHLVEIVEIPGHPWFVATQFHP
                     EFKSRPLRPQPLFRDFVGAAVKYSKGN"
     gene            5825..6475
                     /locus_tag="VF531_01675"
     CDS             5825..6475
                     /locus_tag="VF531_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7712705.1"
                     /translation="MARNRYPEVTVNRILDTSMKLFMSKGYEHTTIQDIIDELGDLSK
                     GAIYHHFKSKEEIMDAVNKRLAEQGIGDLKTIAVDSHLTGIDKLRKMILFSISAAQHE
                     AIDKTVPSFLKNPQLLALHMRDTLGSASDLLTDVIEEGIRDGSIHATQPRELSQLILL
                     FFNVWINPWMYSWTSDEMKSIVTFMKNLFDQIGVPIFSIELLQSLNVLYSLSQDEK"
     gene            6565..7788
                     /locus_tag="VF531_01680"
     CDS             6565..7788
                     /locus_tag="VF531_01680"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7712706.1"
                     /translation="MSSRAFGGKWEKSFLVIAIGEAISEIGSYAVQFSLLWWIASKTS
                     SPLMLALSGLLAFLPQLFLGPFVGVWIDRLKRKKVIIFADLFIGVVATVYAVFFLFGN
                     PPYWSACVVLGIRAIANVFYTPAIQSVIPMLVPKQDLIRANGWLQFMQSGTLMLGPVL
                     GAALYAVLPLPVILLSDLAGALVASVSIGIVKIPELKREQHQARDFMKEMREGFSIFL
                     RDKKLLIVTLAAAIGMIFYMPLSSFFPLMTSGYFHASAWYAGIVQFGYAGGMMICSIL
                     AGAYGKIKNKLLAVHIGLLGLGLTSFGSGILPQNDTAFWIYAVLCMLMGASGNLYNIP
                     YIAYMQETIPKEAHGRAFSVMSSLMSATMPLGLLVAGPVAEINGVASWFFITGVAFFI
                     ITGVSFFLIQLQNIK"
     gene            7886..8443
                     /locus_tag="VF531_01685"
     CDS             7886..8443
                     /locus_tag="VF531_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7712707.1"
                     /translation="MKIQIIGASGTGKSTLARYLSEKTGVYWIDTDKYLWKDSSFTEN
                     YPVEDRFRMYNDDMANHQDYIVSGSVHSWNPGGFNDRELLVLLMVDEDTRMKRIYDRE
                     FASFGARMLPGGDHYQLTREFLDWCRTYLTEDENTIDSLANHRLRLQEASCKTLVLDG
                     NLPVDALYAHVLNAYWADRMNDPTK"
     gene            8562..9320
                     /locus_tag="VF531_01690"
     CDS             8562..9320
                     /locus_tag="VF531_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX7712708.1"
                     /translation="MNYQEGVVITATELKQNLGKYMDYVEQQNDVVITKNGNKIARLT
                     PYVTDIEQYFTVREKALDYQYGGKKVSYEEFMEIYEKSTLRMELINGEIYLLSSPTIG
                     HQDLLGKLYLIFTGYFKNKKCRVFLAPFDVHFRKKDFKEPDVMQPDLLVACDLENNVT
                     EKGKYMGTPTLVIEILSDGTRSKDMIDKLNTYRLSGVKEYWIVDQKQENIMVYGFDNF
                     EIDKYKTYEKGDLAQSLAFNGLAADIAGLFSEML"
     gene            9713..10933
                     /locus_tag="VF531_01695"
     CDS             9713..10933
                     /locus_tag="VF531_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7712709.1"
                     /translation="MNPNWKKNTILFLTSQAISLFGSALVQHAITWYITLKTQSGMMM
                     TIAIICGFLPTFFLAPFAGVWADRYHRKTLIILSDSLIAVATLTLAVLFLTGHNALWL
                     LFVASVIRSFGAAVQTPAVGAFLPQLVPGDKLTRVNAVNSSIQSLMMLIAPMLSAVLL
                     TVTTIEIIFFIDVVTAAIAVAILLLFLHVPAHAKALSKQAVGYLEDLREGIVYIKNHD
                     FVKTFFLFDAFFFILAAPVAFLTPLQVTRSFGGDVWRLSAMEVIFSSGMMAGGIIMAS
                     WGGFKNKVHTMVLSNLVIGVSTFALGIVPFFWLYLGFMALIGLVMPVFNTPATVLLQQ
                     KVEEAFLGRVFGVMGMIASSMMPLGMLVFGPVADFVKIEWLLIGTGLLLVVQSLLMLG
                     NKVLIKAGEPAQEE"
     gene            11201..11947
                     /locus_tag="VF531_01700"
     CDS             11201..11947
                     /locus_tag="VF531_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7712710.1"
                     /translation="MDKFDQYIRSIELNRAGVPSFREYPFALPAVRNLDALPFHPKVT
                     FFVGENGSGKSTILEAIAVAYGFNAEGGTRNFNFSSRATHSELNQYLKLIKGVKRPRD
                     GFFLRAESFYNMATNIDDMDAEPSGAPPVIDSYGGRSLHEQSHGESFFAVFQNRFGGR
                     GLYILDEPEAALSPSRQMAILSRMHELVSQDSQFIIATHSPIIMAYPDAWIYQIREEI
                     KLVKYEETENYRITKAFLNDTRKMLEILME"
     gene            12250..13206
                     /locus_tag="VF531_01705"
     CDS             12250..13206
                     /locus_tag="VF531_01705"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEX7712711.1"
                     /translation="MRLNYPKRLNPPSFLEFQKRFSSEESCAEFLAKQRWPNGWVCPR
                     CNSTKAYWITARKLYECSKCHSQVSVTARTIFHKTRTPLQLWFWAAFLITTDKRGISS
                     VGLGKQLGLSQKQAWFMLHKIRQAMSWREERYQLDGVVELDECFFDAPKARGSLGSSS
                     AKIKVLVGLSNDDQGKPRYIKFKVLTQINRAQMESAVKKMIAPGARIKTDGLRCYLSL
                     PSQGYRHERVRAREKNILKEMRWLHTMISNAKTLIGGTYHGLGSKHLQVYLDEFAYRF
                     GRRHMLDRIVDRCILAMATSPIWTYRNLIEKVDIIDTDKNAA"
     gene            13271..>14140
                     /locus_tag="VF531_01710"
     CDS             13271..>14140
                     /locus_tag="VF531_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712712.1"
                     /translation="MKFKTIHPFLLLLIFCTLAPLSPAADILHRLTHGDQYALVLGRV
                     TQVQKLSFELSVARTVSGHRTPAHILINCSPKGQQDWPKPGEKLLVSLEREAKEYRIH
                     WGLFHVSSLDPRQLKIIQSAWPKGDLAALEHYLHTGENDFFFVYDSAFARFPDGTFTR
                     IYPALPAPQKSKHFKLMICPERREYRLTDSSTPGIGLEAIYFRNSQELKVTWETNYGR
                     FILWEPPEYRVISMGKTAEVPGWKTVYWTPLTMGKDLPKEGIRIGVSVNDQKDKVKVE
                     QAYIEVQFKEGTFR"
BASE COUNT         3540 a         3147 c         3655 g         3798 t
ORIGIN      
        1 cgcctggttt ctcatcatct ccggagtggt ttggggcggg attcagggcc ttttcatgaa
       61 ttataatctc cagaccattg cgctcctctg gttcctgttg ccactcttct ttctccccgg
      121 gatgttacgt gtggtcaatc agtatgaacg gggcgtgctg tttcgcttcg gccgccaagt
      181 gggagtgctc gatcccggga tgaacatcat ttttccgatg ggaatcgatt tcgtacggat
      241 cgtcgatctc cggaccttca ccattgatgt ccccaagcaa gaggtgatca ccagggacaa
      301 cgtgaccatc aatgtggacg cggtggtcta tttcaatgtc ttcgatccgg tattggccat
      361 catcaaggtg gcggatcatg tccgcagcac ttcgctgttg gcgcagacca tactccggtc
      421 ggtcctgggt cagcatgaac tggacgagat gctggccaaa cggaccgagt tgaatgagat
      481 cctgaaaaaa ttgctggatg aggcgaccga cccctggggt gtcaaggtta ccgcggtgga
      541 gattaaggcg gtggaattgg cggaatccgt gaaacgggct atcgcccggc aagctgaggc
      601 cgagcgggaa cgcagggcca aggtaatcgc ggccgacggc gaacttcagt catccgagaa
      661 actctccgaa gccgcccggg ttctcgcgac gcaaccggct tcaatccaac tccgttattt
      721 gcagactttg acggagatcg cggtggagaa aaattcgacg attctattcc cgatcccgat
      781 cgatcttttc cagggcatgc ttcctaaagg aaagcaggaa taggttaaac ttttataaaa
      841 agcctcatca aaggcttcat aaaaagcatc cctcgacttg acaattaagg atctttcccg
      901 taagatatta tttgtttgga tcggtgtaag ggaggtgaag ccaatgccga cgtatgaata
      961 tgtctgtccc aaatgcgggg ttttcgagga gtttcatagc atcaccacgc aactgacccg
     1021 ttgccccaaa tgtaacagcg aagtccgtcg tctgatcagc cagaacacga atgttatctt
     1081 taaaggctct ggcttctata ccacggatta ccgatcggga agtcattcga gttccagtgg
     1141 gacttcgtct ccaaaaacgg acacggcgtc cccgtcttca aaacccgaaa gtaaggcatc
     1201 ataaattaaa ggtcacttcg gtgacaaggt cactgcggtg gcctttctct ttatttagat
     1261 atagtgaact cccgatggtt gggagcgcat gatctatcaa agcgaatcat atggttgata
     1321 ataaaattga cctcaaattt tatggaaacc ggagagatag aggaacgcat ggaacagatc
     1381 gtaaccagaa gagtgctgca aaagttgagg gagtccctga ccactgcggc ggaacaggcc
     1441 cgttcccagg gacggcttgt ttttgaggaa cttcccgaat ttacgctgga agtaccgaaa
     1501 gataaaaacc atggtaattt cgccaccaac ctggcgatga tgttgtccaa acccaaccgt
     1561 aaacccccac gcgaactggc ggaattgatc attgccaatc tgccgtcaaa tccctattgt
     1621 gaccgggtgg aagtggcggg cgccggcttt atcaactttt acctgaacaa cacctggctt
     1681 ttggaggtgg ccgagtccat tctcaccgaa aaggagcggt tcggcaataa cgcggattta
     1741 accggcttca aggtacaact ggagttcgtc agcgccaacc cggtgggtcc gatgaatgtg
     1801 gtcaacgcca gggctgccgc cctgggggat acccttggca atctgtttac agcttgcggc
     1861 gccgaggtgg aacgggagta ttatgtgaat gattttggcg ttcaggttac cgctttgggg
     1921 cgctccctcg atgcccggta ccgggaatta ttgggccaga cggtggagtt tcccgaagac
     1981 ggctatcagg gcgagtacat ttacgatatg gcccgggagc tcattgacgc ccagggcgat
     2041 tcctatctga agctgtccga aaccgagcgg atccaaactt tccgggatct gggctatcgc
     2101 cggattttga agacccaaaa gaccgatctg gaaaattacg gtgttcatta cgattgctgg
     2161 ttcagtgagc gcagcctgca tgaaggcggt gaggtggcca aggttttaca gaccttgacc
     2221 gaccggggtc tcacttacga acaggatgga gcgttgtggc tggcgacgtc aaaatacggg
     2281 gatgacaagg atcgggtgct ccggaccgcc gatggccgga ccacttactt cctttcggac
     2341 atcgcctatc atctgaacaa gttcggccgg ggtttcgagc gcgtcatcga catctggggc
     2401 ccggatcatc acggttacat tccgcggatg aaagcggcca tggctgcgat ggggatcgag
     2461 ccagaccggc tcgaggtgct gatcgcccag cagatcaacc tgctcaaaga ggggaaaccc
     2521 ttcaagatgt cgaagcggcg cggtgaattc attaccatga ccgacctatt gcaggaagtt
     2581 ggcaacgacg gggcacgctg gtttttcctg atgcgtagtt ccgacagtca cctggatttc
     2641 gatctgggtt tagccaagtc tcagagtaac gagaacccgg tcttttacgt tcaatacgct
     2701 catgcccgga tttgcagtat ttttaagcaa tctgccggcg gttcccttcc cggtccggag
     2761 gatattcccg ggctttcacg ccactgggac gaggaggaac aggcgcttct tgagaaagcg
     2821 gccttattcc cggaactcgt ggtcggggcg gctcatcatc gtgaaccaca ccgtttaaca
     2881 ggctatctcc tggagctggc cacgctgttc cattcatatt ataaccggcg ccggatcatc
     2941 gccgaggagc cggaggtaac acgggcccgg ttagctgtgg ccgagagtct gggttacatc
     3001 tttcggcgcg gtttggaatt gcttggcgtt tccgctcccg agcatatgta agatttcgga
     3061 tacaactgtt ctcatatcga tggggatttg gtttgagatc atccaaacgt catttcaatt
     3121 tgacctccgg acccggtatt gaatgaggac ccggcggttt gagaatagga ggagatcatg
     3181 agaaaattac cgttattccc gaccctcgga gattcactga aaaaaacctt taaagagctt
     3241 tatagttcaa tggggttctc gtttttactc agcctgcttt ggtttaccgc ctatctaccg
     3301 attattttcg tttttatctc gatctatttc aatcccaccc tgttatcaaa ggttaaacaa
     3361 cccggggata tttttgtact gtttttcggg atgtccttgg tctgtacgat atggaacgga
     3421 ctggtggccg gtccggtgac cactgcgctc tacggtatct acgagaatcg taagggcgat
     3481 tacccgagca cccgtatgtt ttttcagatc ttcaaaaagt gctatacccg ttcggcaggg
     3541 gttcattttg tttactcgct ggtcgtcacc gcattgcttt tgaactttgt cattatcttt
     3601 acccggccga atgtcctgat gctgatcgcc gggatcatct cgctgtatat gctgtttttc
     3661 ctggcattgc ttcagtttta ttttcatccg ttgcttttcc tggataatgg tttcaaaaaa
     3721 gtcgtcaaga agtctttctt actgacagtg gacaacctgg ggttgagttt ttggtatgcg
     3781 gtgatcatcg gattccttcc ggttttgacc ttgctcattc cgcagatcat gccggtcaca
     3841 atgttggttc tgttcttcat ttacggcgcg tttatcattt atatcatcaa tcacggattt
     3901 gaaaccatct atgcgaagta tgacgagtga gtcattctat tctttgaaac tggaggtttt
     3961 ttcgtaatgg ctaagtttat atttatcacc ggaggcgtcg tctcttccct gggcaaaggt
     4021 atcaccgcag cttctctagg gtgtctccta aaaagcaggg gaatcaatgt ctccatcttg
     4081 aaactggatc catacatcaa cgtcgatccg ggaaccatga gtccttacca gcatggcgag
     4141 gtgtttgtga ccgacgatgg agcggagacc gacttggatc tgggccatta cgaacggttt
     4201 atcgatgtaa acctcagtaa gaacaataat gtgaccaccg gcaagattta ctggtcggtc
     4261 attaccaagg aacgcaaagg ggattatttg ggcggcaccg tgcaagtcat tccccatatc
     4321 accaatgaga ttaaagaccg gatccatcgg gtcgccaagg agagccaggc cgatgtagtg
     4381 ctggtggaag tcggcggcac ggtgggtgat attgagagct tgcctttcct ggaagccatc
     4441 cgccagttta agggggacat cggccgggat gacgtgatgt acatccacgt gaccctgatg
     4501 cccttcatcg gcgcggcctc ggagctcaag accaaaccga cccagcatag cgtcaaggag
     4561 ttgcggagca tcggtattca accggacgtg atcgtctgcc gctccgaaaa acccatttcc
     4621 aatgagctca aagccaaaat cgcccaattc tgtgatgtca acaaggaagc ggttattccg
     4681 aatctggacg ccaagaccat ttatgaagtc ccgctccttt tggaacaggc cggcctggat
     4741 cagatcgtga tcgaacggtt gaaactcctg tgcaaggaaa gagatctcca ggtctggcgg
     4801 gaaatggtca gcaagatgaa atctctcaag gggaaggtga ccatcgccat cgtcggtaaa
     4861 tatgtctcgc ttcccgacgc ctatctcagc gtggctgagg cattgcgcca cggcggaatc
     4921 gtccatgata cccaggtcca gatcaagtgg gtgaattccg aggttattga gcgggacggt
     4981 gtggagaagg tcatcggcca ggttgacggg atactggttc cgggcggatt cggcaaccgg
     5041 ggaatcgagg gcaagatcga agcggtccgt tgggcccgcg agcagaaaat tccatatctc
     5101 ggaatttgct tagggatgca atgcgcggtc atcgagtatt cccgaaatgt ctgccacctg
     5161 gaaaaagcca actcctccga gtttgatccg gattcgcccc attgcgtggt ggatcttctg
     5221 ccggaacaga aggagatcga ggacatggga ggcaccatgc ggttgggtct cgatccggtg
     5281 cagatctccc aggaaaccct ggcccacaag gcctatcagc aggagttaat ctatgagcgc
     5341 catcgtcacc gctatgaggt aaataacgag tatctttcca aactgcaaca ggcgggcctg
     5401 ttaatcagcg gcttcctgcc cgaccgtcat ctggtggaga tcgtcgagat tcccgggcat
     5461 ccctggttcg tggcgaccca gttccatccg gagttcaagt caaggccgct ccgcccgcaa
     5521 ccgttattcc gcgatttcgt aggcgcggcg gtcaaatatt caaagggaaa ttaagtaata
     5581 tcacaacttc attgctgtgc ttttttggaa cgtgatttta agtatggtag ccccgggacc
     5641 agtttcttta gttacccggg gttttttata tacaatggat taggttataa ggtaggattt
     5701 gcgtaataaa ggctgtcgga ggatttggcg aagaggaata tacatcgtat agtattgaca
     5761 aaaaatgatt gtcctgttat tatacatacc aaaagtatgt ataatagaaa cggagtaaag
     5821 tcttttggca cgcaacaggt atcctgaagt aactgtaaac cgcatccttg acacatcaat
     5881 gaaattgttc atgtcaaagg gttatgagca cacaacgata caggacatca ttgatgaact
     5941 gggagattta agcaagggtg ccatttatca tcattttaaa tcaaaagaag aaattatgga
     6001 tgcagtcaat aaaagactgg ctgaacaggg gattggagac cttaaaacaa ttgctgtcga
     6061 tagtcattta accgggatcg ataagcttcg taaaatgatc ttattttcca tatctgcggc
     6121 tcaacatgaa gctattgaca agacagtgcc ttctttcctt aaaaatccgc aattattggc
     6181 gcttcatatg cgcgatacgt tgggctcggc atccgatctg ttaaccgatg taatcgaaga
     6241 gggcattcgt gacggctcaa tccacgcgac acagccccga gagttatctc aattgatatt
     6301 gttatttttc aacgtctgga tcaacccatg gatgtattca tggacatccg atgagatgaa
     6361 aagtatcgtt acctttatga aaaatctttt cgatcaaatc ggagttccta ttttttcaat
     6421 agagctcctg caatcgttaa atgtattata ctcgctttca caagatgaga aatagctatt
     6481 ctatggtgat tgagaatagt gtttttttat ccttatatac ataccgaacg acggtatcta
     6541 atttacatga aaggatctgt tgatatgagt tccagagctt ttgggggtaa atgggagaag
     6601 agttttttgg ttatcgctat cggggaggct atctcggaga taggcagtta tgcggtgcaa
     6661 ttctcactgc tttggtggat tgccagcaaa acatcgtcac ctttaatgct ggcattatcc
     6721 gggttgttgg cctttttacc tcaattattt ttaggcccgt ttgttggggt atggatagat
     6781 cgcttaaaac gaaaaaaagt tattattttt gcggatctat ttattggagt ggtggccaca
     6841 gtttacgcgg tattttttct atttggcaat ccgccgtatt ggtcggcctg cgtcgttctc
     6901 ggcattcgcg ccatagcgaa tgtcttttat accccggcca ttcagtcggt aatcccaatg
     6961 cttgttccaa agcaggatct gattcgcgct aacggatggc ttcagtttat gcagtcggga
     7021 acgttaatgc tggggccggt tttgggggcg gcgttgtatg cggttttacc tctgccggtc
     7081 atattattat ccgatttagc cggtgcgctt gtggccagcg tttctattgg gattgttaag
     7141 attcccgagt tgaagcgaga gcaacatcag gcccgggatt ttatgaaaga aatgagggaa
     7201 gggttttcca tatttcttcg ggataaaaaa cttttgattg ttacccttgc cgctgcaatc
     7261 ggtatgattt tttacatgcc gctttcatca ttttttcctc tgatgacaag tggctatttt
     7321 catgcatctg cgtggtatgc aggtatcgtc caatttgggt atgcaggagg gatgatgata
     7381 tgctctatat tggcaggagc gtatgggaag atcaagaata agttgttggc ggtgcatatc
     7441 ggcctcctgg gattagggtt aacttctttc ggtagcggta tccttcctca aaacgacacg
     7501 gccttttgga tatatgccgt attatgtatg ctcatgggtg caagcggcaa tctgtacaac
     7561 attccttata ttgcctatat gcaggaaacc ataccgaagg aagcgcacgg acgcgcattt
     7621 tcggtaatga gcagcctgat gtcggctaca atgccgctgg gtctcctagt tgcggggccg
     7681 gtagcggaaa tcaacggagt agcctcgtgg tttttcatta ccggagttgc tttttttata
     7741 attacgggcg ttagtttttt tctcatccaa ttgcaaaata taaaatgata atatgttaag
     7801 aaagaagaga tgacggagtc agtccaaatc ttacccggta aattatgtgg aaaaacctta
     7861 aataggggaa tacaaaggtg ttaagatgaa aatacagatt ataggggcct ccggtacggg
     7921 aaaaagtacc ttagccagat atttaagtga gaaaaccggt gtttactgga ttgatacgga
     7981 caaatatcta tggaaagaca gctcctttac tgagaattat ccggtcgagg accgctttag
     8041 gatgtataat gatgatatgg ccaaccatca ggattatatt gtgtctggtt cggtacattc
     8101 ctggaatcct gggggattca atgacaggga gttgctggtg ttactgatgg ttgatgaaga
     8161 tacccggatg aaaaggattt atgacagaga gtttgcaagt tttggagcaa ggatgcttcc
     8221 gggtggggat cactatcagt tgacccggga atttcttgac tggtgtagga cttatctgac
     8281 tgaggatgaa aatacaattg attcccttgc caaccatagg cttcgtttgc aggaagcatc
     8341 ctgtaaaaca ttggttttag acggaaatct gccggtagat gctctttatg cccatgtttt
     8401 aaacgcctat tgggctgacc ggatgaatga tcccaccaaa tgaattattc aaagtatcgt
     8461 acaagataat ttaataatat cgcacataat atatcgaaat atagcacgat attattgtgt
     8521 gaaaccgccc attacagtat aatgattatg ggggtgatga tatgaactat caggaaggcg
     8581 ttgtaataac tgcaaccgag ctcaaacaga atttaggaaa gtatatggac tacgttgagc
     8641 agcaaaatga tgtggtgatc accaaaaacg gcaataaaat cgccaggttg accccttatg
     8701 taaccgatat cgagcagtat tttacagtga gagaaaaggc gctggactat caatacggcg
     8761 ggaaaaaggt ttcctatgaa gagtttatgg agatttatga aaaaagtacg ctcagaatgg
     8821 aattgattaa tggtgaaatc tatttactat cttcgccgac cataggacat caggatttac
     8881 ttggcaaatt gtacctgatt tttaccggat attttaaaaa taaaaaatgc cgggtctttt
     8941 tagccccttt tgatgtgcat tttagaaaga aggattttaa agaacccgat gttatgcagc
     9001 cggatctgct ggtggcctgt gacttggaga ataatgtcac ggaaaaaggc aagtatatgg
     9061 gcactccgac tttggtgatt gaaattttgt cggacggcac ccgcagcaag gatatgattg
     9121 acaagttgaa tacttaccgg ctatcggggg tcaaggaata ctggattgtt gatcaaaagc
     9181 aggaaaatat catggtgtat ggctttgata attttgaaat cgacaagtat aaaacctatg
     9241 aaaagggtga tctcgcccaa tcactggcat ttaacggctt agcggcggat atagccggtt
     9301 tgtttagtga aatgctttaa atgaggctgt catattctat tccgagacaa aacagaacca
     9361 aatattttga gaatcaaacg atcacttgaa tgtccttgaa atattcgtcc ccatatggca
     9421 taataaatct aagtctatga acgggggaca cggattccat gcatttcatt cgtttaagat
     9481 aagctgacaa taaaaaaagt tatctatcca ccaaaagtgg gcttttttgt tgtgcttatt
     9541 tttgcgaatc aagacatgga ataaaggacg tgttcctgct tgggaattcc tctttattta
     9601 atgatctttt gatcgcggat aggcagatca aagcccgttt ggcggacgat ggtctgcttt
     9661 tttattaccc aaatccattc acgacaatct atcaatgatc ggagaataaa tcatgaatcc
     9721 aaattggaaa aagaacacga ttctcttttt gacaagccag gcgatatccc tcttcggctc
     9781 ggcattggtt caacatgcca ttacctggta tattacgtta aaaacccagt ccggcatgat
     9841 gatgaccatc gccattatct gcggttttct acccaccttt tttctggcgc cctttgcggg
     9901 ggtatgggcg gaccggtatc atcgtaaaac gctgattatt ttgtccgact cactcatcgc
     9961 cgtcgcgacg cttactttag ccgttctatt tttaacagga cacaatgcgc tatggttgct
    10021 gtttgtggcg tctgtgattc gctcctttgg ggcggcggtt caaacgccgg cagtgggggc
    10081 ttttctgccg cagctggtgc cgggggataa actcaccagg gtaaacgcgg ttaacagcag
    10141 tattcaatcc ctgatgatgc taattgcgcc gatgcttagc gccgtcctgc tgacggtgac
    10201 caccatcgag atcatcttct ttatcgatgt agtgactgca gcaattgcgg ttgcaatttt
    10261 gcttttattt ttacatgtcc cggcccatgc caaagcctta tcaaagcaag ccgtcggcta
    10321 tttggaggat ctgcgcgaag gtatcgtcta tatcaaaaac catgattttg tgaagacctt
    10381 ttttctattc gatgccttct tctttatctt ggccgctcct gtggctttcc tgacgccgct
    10441 gcaagttacc cggagtttcg gcggggatgt ctggcgcctc tccgcaatgg aagtaatttt
    10501 ttcctccggg atgatggccg gcggaatcat tatggcttcc tggggcggtt ttaaaaacaa
    10561 ggttcatacc atggtcctgt ccaatctggt catcggcgtt tcgacctttg ccttgggcat
    10621 agtgccgttt ttctggctat atttggggtt catggcctta atcggattgg tgatgccggt
    10681 ctttaacact ccggcaacgg ttttgctcca gcaaaaggtg gaggaggctt ttttgggaag
    10741 agtcttcggg gtgatgggca tgatcgcaag cagtatgatg cccctgggaa tgctggtgtt
    10801 cggaccggta gccgacttcg ttaaaatcga atggctgttg atcggaaccg ggttgttact
    10861 ggttgtccag agcctattga tgttgggcaa taaagtgctg atcaaggccg gagaaccggc
    10921 acaggaagag taagggtggg cggttgactg gttttgacgg cagcgatata agggcggaga
    10981 ggttcaatgg atgaatggtt gattgtaaat atcgactcgg actcataacc caagctttgg
    11041 gcattggtgt cgaccgaagg gatcgagaaa cttttggggg aaaatattta gggtcccaaa
    11101 gcatttggga gggggatagc gtgaatattt acggccattg agaagtgtgc cagggaaagt
    11161 agatggaatg atattaggag cgtaatgagg gaatcttgat atggataaat ttgatcaata
    11221 tatccgaagt atcgaattaa accgggccgg agttccgtca ttccgggagt atccttttgc
    11281 attaccggcg gtgagaaatc tcgacgcctt gccgtttcat cccaaagtca cctttttcgt
    11341 cggtgaaaac ggttccggaa agtcgacgat tttggaagcg attgcggtgg cgtatggttt
    11401 taatgccgaa ggcggaacga ggaacttcaa tttttcatcc cgggcgaccc attcggagtt
    11461 gaaccaatat ctgaaattga tcaaaggcgt caaaagaccg cgggacgggt ttttcttaag
    11521 ggcggagagc ttttataaca tggctactaa tattgacgat atggatgccg aaccgtcagg
    11581 cgcgccgccc gtcattgact cctatggagg ccggtcgctt catgagcaat cccatggcga
    11641 atcgttcttt gcggtttttc aaaaccggtt cggtggccgg gggctctata tattggatga
    11701 acccgaagcg gccctgtctc cttcccgcca aatggcgata ctcagccgga tgcacgagtt
    11761 ggtgagtcaa gattcccagt ttatcatcgc cactcattcc ccgatcatca tggcctatcc
    11821 cgatgcatgg atttatcaga taagagaaga gatcaagctg gtaaagtatg aggaaaccga
    11881 aaactaccgg attaccaagg catttttaaa cgatacccga aagatgctgg agattttgat
    11941 ggagtgagat ttcccaccgg ttcgacgaag gccctcatcc ctggggttgg ttcatcgcgt
    12001 gctctgcacg cgcgccttcc cctgcgattg cgtgggaagg atcaggacgg tgaatttatg
    12061 ataggctgtc tttttcctcg cccgtggtaa cgcgtggtaa cgggagaggg ccaacaattg
    12121 tgaatgagta gatagggtga gaattactat cccgttaaat caggtcggga aaaccgcttg
    12181 acaacaagca tttattcgaa ttataataaa attatgaatc gaacaaatgt tcaaaaggtg
    12241 gtggccaata tgcggttgaa ctacccaaag cggctaaatc ccccatcgtt cttagagttt
    12301 cagaaacgtt tctcctctga agagtcatgt gccgaatttc tggctaaaca acgctggcct
    12361 aatggctggg tttgtccaag gtgcaattcc accaaggcat actggataac cgcccgcaaa
    12421 ctgtatgaat gttccaagtg tcattctcaa gtatctgtga ctgcccgaac tatttttcat
    12481 aaaacccgga ctccgctaca actttggttt tgggcggctt tcttgataac aacggacaaa
    12541 agaggcatct cctcggtggg cctcggtaag cagttaggtc tttctcagaa gcaagcctgg
    12601 ttcatgttac acaagattcg tcaagccatg tcctggaggg aagaacgtta tcagttggat
    12661 ggtgttgtcg aactggatga atgtttcttc gatgccccaa aagcaagagg ttctctcggg
    12721 agcagttctg ccaaaattaa ggttttggtg gggctttcca acgatgatca aggcaagcct
    12781 cggtatatca aattcaaagt cctgactcag atcaaccggg cccaaatgga atcggcggtc
    12841 aaaaaaatga ttgcgccagg tgccaggatt aaaacagatg gtttacgctg ctatctttct
    12901 ctaccgagcc agggttatcg acacgaacgg gtgagagccc gggagaaaaa tattttgaaa
    12961 gaaatgcgct ggctacatac aatgatatcc aacgcgaaaa cactaattgg cggtacttat
    13021 cacggacttg gcagtaagca ccttcaggtt tatttggacg aatttgctta tcgctttgga
    13081 cgccgtcata tgctggaccg gattgtcgat cggtgcatac tggctatggc tacgagtccg
    13141 atttggacct atcggaatct cattgaaaag gtcgatatca ttgatactga taaaaatgct
    13201 gcttaatcgc agatagattt gaattggtat tcctgagtta agaggatagc ttatgatagg
    13261 ggggaataaa gtgaaattca aaaccatcca tccgttccta ttgcttttga ttttctgtac
    13321 cctcgccccg ctttcccccg ccgccgatat tctccaccgt ctgacccatg gcgaccaata
    13381 cgccttagtc ctgggtcggg tcacgcaggt tcaaaaatta tccttcgaac tctccgtcgc
    13441 ccggaccgtc agtggacacc gtacgccagc ccatatattg attaattgtt ccccaaaggg
    13501 tcagcaggat tggcccaagc ccggtgagaa gctcttggtc tctttggaac gtgaagccaa
    13561 ggagtaccgc atccactggg gcctgttcca cgtctccagt ctagatcctc gtcagttaaa
    13621 aatcatccaa tccgcctggc ccaaaggcga tctggccgct cttgagcatt accttcacac
    13681 cggagagaat gattttttct ttgtctacga ttcggccttc gcccgtttcc cggacgggac
    13741 ctttacccgg atctaccctg cgttgcccgc tcctcaaaag tccaagcatt tcaagctcat
    13801 gatctgccct gagcgtcggg agtaccgcct gaccgactct tcgacaccgg gaatcggcct
    13861 ggaggcgatt tatttccgta actctcaaga actcaaggtt acttgggaga cgaattacgg
    13921 ccgcttcatc ctctgggagc cccctgagta ccgggtgatc tctatgggaa agacggccga
    13981 agtcccaggg tggaagacgg tctattggac accgttgacg atggggaagg atttaccgaa
    14041 agaggggata cggatcggtg tgagtgtgaa cgatcagaag gataaggtga aggtggagca
    14101 agcttacatt gaggtccaat tcaaagaagg gacttttcgg
//
LOCUS       DASVMB010000049         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_4727,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000049 DASVMB010000000
VERSION     DASVMB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4727"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF531_01715"
     CDS             <1..348
                     /locus_tag="VF531_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7712713.1"
                     /translation="TGLGSTAWLKSILVGATGIVQSISGSQQIKVKPEMKLSWNADYL
                     LFSVREPFPSKTSGANLVFGKISPKIPLKILSQMPENGVIFSDGMENDYLSFNSGIEA
                     TISVAERKGRLVV"
     gene            660..1112
                     /locus_tag="VF531_01720"
     CDS             660..1112
                     /locus_tag="VF531_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7712714.1"
                     /translation="MGPLENDSVYTLFGRVVRLHYCRMHMLLEKVGVYPGQPPLLLTL
                     GKTDGQSQRELAEKLRIKAATITVMVKRMESAGLVERRNDPDDQRITRVYLTDLGRVV
                     LGKVRETLKAMEGECFCNFTAEEQIILRRLFMQMRENLLKVCGKEADS"
     gene            1278..1886
                     /locus_tag="VF531_01725"
     CDS             1278..1886
                     /locus_tag="VF531_01725"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7712715.1"
                     /translation="MQTDRKTEILDAFMRLVSQYGIDKTTMQDIAREAEINVALIYKD
                     FRNKEDLVEAYIYRKIQQIMVECEQIQKQDLPPEQLLHEFISGLFRVIQSSVLQDKGF
                     WQFVRDENTIKYLRRMIHKKDSFLQANIKMIEEIMARGVREGVFEIEDLHETAELFLD
                     AFHKYFGMMFKCRTEADLFSGIDKMYALLIKGIRKKAPAARV"
     gene            1953..3680
                     /locus_tag="VF531_01730"
     CDS             1953..3680
                     /locus_tag="VF531_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712716.1"
                     /translation="MIKLFRFLKPYILPIVVVVLLVFIQSMAELYLPNLMSDIVNKGI
                     TNNNIDYITQTGGFMILITLVDSLCAILAAFLSSQIAMGFGRIIRGELFAHITRFSLH
                     EFDKFGTPSLVTRNTNDITQIQTVVMIMLRMMIMAPITCVGGIIMAVSKDKGLAWIIM
                     VVVPVLAISVAFIARKAFPLFKSIQAKIDKINLILREELTGIRVIRAFNRSDDERLRF
                     GKANLDLTDTSIQVNRILALLMPVLMLVMNFTSLGVLWFGGLRIDLGTSNVGDMMAFL
                     QYAMQIMFAVLMGSMMFVMLPRAQASAVRINEVLATVPEINDAKTVKKPGLKRGYVEF
                     KNVSFSYHGAEEPAIREISFSAAPGEITAIIGSTGSGKSTLISLVPRFYDVDSGSVEI
                     DGLDVREMSQDTLRSKIGFVPQKAFLFTGTIADNIRYGKEDASDEEVSHAAEIAQATE
                     FIASMQEGFDTVIAQGGTNVSGGQKQRLSIARALVGKPEIYIFDDSFSALDFKTDARL
                     RAALKKETADATVIIVGQRVATIMDADRIIVLDEGRIAGIGTHKELYKNCEVYQEIVH
                     SQLSENEIA"
     gene            3775..>4562
                     /locus_tag="VF531_01735"
     CDS             3775..>4562
                     /locus_tag="VF531_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712717.1"
                     /translation="MSEEQKSAQSSRRGMGPGGFGGGHGPMGAMAGGKAKDFKGSLKR
                     LIAYLHPHRVQLLIVIVFAVLSTTFTIVGPKILGKATTRLLEGIISKFAAYQLHKPVP
                     SMDFIYIGRIILIMGCLYIISAVFSFIQQYIMAGVAQKTVFDMRRDVNDKLARLPMKY
                     FDSRTHGEIMSRVSNDIDNISTTLQQSMTQLITSICTVIGVVIMMLTISPMLTLLTVL
                     VLPVSMAATMAIIKRSQKYFVEQQKELGELNGHVEEMFSGHAIV"
BASE COUNT         1117 a         1043 c         1222 g         1180 t
ORIGIN      
        1 accggtttgg gatctacggc ttggctcaaa agtattttag tcggggcgac cgggattgtt
       61 caaagcatct ccggatcaca acagataaaa gtcaaaccgg agatgaaact gagttggaat
      121 gccgattatc tgctcttttc cgtccgggaa ccgttcccca gcaagacatc cggtgcgaat
      181 ctggtatttg ggaagatatc cccaaagatc cctctgaaaa tactttcgca aatgccggag
      241 aatggggtca ttttcagcga cggaatggag aacgattatc tcagtttcaa ctcgggaatt
      301 gaagccacga tttcagtggc ggaaaggaaa ggccggttgg ttgtttagta ggggtggctt
      361 tttatcggag tggctttttg ttggagttgc ttcttcatca gtgaacagat agggaaaaca
      421 ggattccgga caggggtcct gctttttatt tatgatttat gcggactgat tgatcctagg
      481 attggcatcc cctgctttag ggagatcttt ttatatcggg taactgaagt tcatcaccga
      541 ttgttaacaa agcgctgttt ggaatcgact gttttttctt gactccctaa ggatgaagtg
      601 gtattatcag ttttgctcga ttttgttagc cgtctaatta attaaggaga agatggacca
      661 tgggcccttt agagaacgat tccgtttata ccttgtttgg ccgggtggtg cggctccatt
      721 attgccggat gcatatgctt cttgaaaaag tgggtgtata tcccggccag ccgccgttgc
      781 tcctgaccct cggtaagacc gacggccaaa gccagcggga gttggcggaa aagctgcgta
      841 tcaaagccgc caccatcacc gtgatggtga aacggatgga atcggccggg ctggtggaac
      901 gtcgcaacga ccctgacgac cagcggatta caagggtata tctcaccgat ctggggcggg
      961 tggtattggg caaggtgcga gaaactttaa aagccatgga aggcgagtgc ttttgtaatt
     1021 ttacggcgga ggaacaaatc attctacgcc ggttgttcat gcagatgagg gagaatctgc
     1081 tcaaagtctg cgggaaagaa gcggatagtt aaccatctga gatggttaac cagcatagat
     1141 ggttagacgg cttacgtttt taatcagatg tttacatggc agatattgac gcgcttggca
     1201 gaaaaggcta ttatttttac tacacggtta aattattaaa taatttattt acttgtttac
     1261 ttgaaagagg tagaccgatg cagacggacc gtaaaacgga gatattggat gcctttatga
     1321 ggctggtgag ccaatacggg atcgataaga ccaccatgca ggatatcgcc agggaagcgg
     1381 agatcaatgt cgccctgatc tacaaggatt tcaggaataa ggaagatctg gtcgaggcct
     1441 atatctaccg gaagatacaa cagatcatgg ttgagtgcga acagattcag aaacaagatc
     1501 ttcccccgga gcaacttttg catgaattta ttagcggatt attccgggtc atccagagtt
     1561 cggtcctgca ggataaaggt ttctggcagt tcgttcgcga cgagaatacc atcaaatatc
     1621 tgcggcggat gattcataag aaagattcgt ttctgcaagc caatataaaa atgatcgaag
     1681 agatcatggc ccggggcgtc cgggaagggg tatttgagat cgaggatctt cacgaaaccg
     1741 cggaactttt tctggacgca tttcataagt atttcggaat gatgtttaag tgccggacgg
     1801 aagccgacct cttctcgggt atagacaaga tgtatgcgtt gctcatcaag gggattcgaa
     1861 agaaggcgcc ggctgcccgg gtgtgaatct tcatccgatt gttctgcgat ttaccgtgtt
     1921 tctgagactt atccaactgg agatggtgat ccatgattaa gttattccgg tttttaaaac
     1981 catacattct cccgatcgtc gttgtagtgc tgctggtgtt catccagtcc atggccgaac
     2041 tttatctgcc caacctgatg tcggacatcg tcaataaggg catcaccaat aacaacatcg
     2101 attatattac ccaaaccggc ggattcatga tcctgatcac cctggtcgat tcgctctgcg
     2161 cgattttagc cgcgttttta tcttcccaga tcgccatggg ttttggtcga atcatccggg
     2221 gcgaactttt tgcgcatatc acccgtttct cccttcatga gttcgacaaa ttcggcactc
     2281 cctcgctggt aacccgaaac accaacgaca ttacccagat tcagacagtc gtcatgatca
     2341 tgctgcggat gatgatcatg gcgcccatca cttgtgtcgg cgggattatc atggcggtat
     2401 ccaaggataa gggtctggca tggattatta tggtggtcgt tccggttttg gcaatcagtg
     2461 tcgcttttat cgcccgcaag gccttcccgc tgttcaaatc cattcaggcc aagattgata
     2521 agataaatct gattttgcgc gaagaactca ccggaatccg ggtcatccgg gcctttaacc
     2581 gcagtgatga tgaaaggctg cgttttggca aggccaatct cgacttgacc gatacttcca
     2641 ttcaagtcaa ccggatactg gcgcttttaa tgcctgtcct gatgctggtg atgaatttta
     2701 cctccctcgg ggtattgtgg ttcggcggtc tccggatcga tttgggaacg tccaatgtcg
     2761 gcgatatgat ggcgtttttg cagtacgcca tgcagatcat gttcgcggtg ctcatgggtt
     2821 ccatgatgtt cgtcatgctg ccccgggccc aagcgtccgc tgtccggatt aatgaagtac
     2881 tggccaccgt tcccgagatc aatgatgcca aaaccgtcaa aaagccgggt ttaaaaagag
     2941 gttatgttga gtttaagaac gtcagcttca gctaccatgg cgccgaggaa ccggccatcc
     3001 gggagatctc gttttcggcg gctcccggag agattacagc gatcatcggc agcaccggtt
     3061 cgggtaaatc gacgctgatc agtctggttc cccgttttta cgatgtagat agcggcagtg
     3121 tggagattga cggtttggat gtcagggaaa tgtcacagga tactttgcgg tctaaaatcg
     3181 gcttcgtgcc gcaaaaggct tttcttttca ccggtactat cgccgataat atccgttacg
     3241 gcaaggaaga cgccagtgat gaggaagtga gtcatgccgc ggagatcgcc caggcgaccg
     3301 agtttatcgc ctcgatgcag gagggttttg ataccgtgat tgcccagggc ggtaccaatg
     3361 tttcgggcgg ccaaaaacag cgcttgtcga tcgcccgggc tttagtgggt aagcctgaga
     3421 tttatatctt cgatgacagc ttctccgccc tggactttaa gaccgacgcc aggttgcggg
     3481 cggccctcaa gaaagaaacg gccgacgcca cggtaatcat cgtggggcag cgggtggcca
     3541 ccattatgga tgccgatcgc atcatcgttc tcgatgaggg tcggattgcc ggaatcggta
     3601 cccataagga actttacaaa aattgcgagg tataccagga gatcgttcac tcgcaacttt
     3661 cggagaatga aatcgcttaa gtatccgaat atgcttaggc aatcgaaaat acgaaggaat
     3721 gcgccgtgaa tgaaccgcgt catacgcggg atccggttaa gaaaggtgac aagcatgagt
     3781 gaagaacaaa aaagcgccca gtcgtcccgg agggggatgg gacccggggg gttcggcggc
     3841 ggccacggcc cgatgggcgc aatggccggc ggcaaggcta aagattttaa agggtccttg
     3901 aagcggttga ttgcctattt acatcctcat cgggttcaac ttcttatcgt tatcgtattt
     3961 gcggtcctaa gcaccacctt tactatcgtg ggcccgaaga ttttgggcaa ggccaccacc
     4021 cgtctgctcg aggggattat atccaagttt gcagcatatc aactgcataa gccggtcccc
     4081 tccatggatt tcatctatat cggcaggatc attctgatca tgggttgcct ttatattatc
     4141 agtgcggtat tcagctttat tcaacagtac atcatggccg gtgtggctca aaagaccgta
     4201 ttcgatatgc gccgggatgt gaacgacaaa ctcgcccgcc tgccgatgaa gtattttgat
     4261 tcacgcaccc acggcgagat catgagccgg gtatccaatg atattgacaa tatcagcacc
     4321 actttgcagc agagtatgac gcagttgatc acctcaatct gcaccgtgat cggggtggtc
     4381 atcatgatgt tgaccatcag cccgatgttg accttgctta ccgttctcgt tctgccggtc
     4441 tctatggcgg cgacgatggc catcatcaaa cgctcccaga aatacttcgt ggaacaacag
     4501 aaggaacttg gcgagctaaa cggacatgtg gaggagatgt tctccggcca tgccattgtc
     4561 aa
//
LOCUS       DASVMB010000050         8575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_79605,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000050 DASVMB010000000
VERSION     DASVMB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79605"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VF531_01740"
     CDS             complement(<1..808)
                     /locus_tag="VF531_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712718.1"
                     /translation="MKQKSDVSTARLSGNPILINLILGVIVLAWLIPTFMLFTSSIRP
                     VGDMLTTGWWTSFLHPERFTLENYREVLASKGIGTGFINSLIIAIPSTVLPIFLGALA
                     AYPISFYKFPGRKLIFIAIIAMQIVPLQTVLIPVLLTLKGLQLNGTYLGIWLAHTAFG
                     LPLCIYLFRNFFAQLPYSLIEAAKIDGANNFQIFMILITPMSKPVFASLAIFQFLWIW
                     NDLLVALVLLGDPALTPLTVRLSSLLGSLDSGWNIMTPAAFASMLLPLTIF"
     gene            complement(832..2031)
                     /locus_tag="VF531_01745"
     CDS             complement(832..2031)
                     /locus_tag="VF531_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712719.1"
                     /translation="MNIGRKNKSNAGQSLEKGHIREWAVNYLWMLPAMILVFFFLVLP
                     TLQTIYLSLHEKVTFSESQFTQDVLGVLKTESGREIKRDDLIERIPGWRKAMNRVAFQ
                     YKIKIAPGDINEEMTVKDTAEMLIVAKNQAQRSEGRDQQFAGLKNYIQMFTDSTMLTA
                     FRNNLIWLLFFTVFTVILGLLVAALADRVRWSTLAKTIIFLPMAISGAAAAVIWNFMY
                     FKDVNTGTINGVINAALHLLQKIGLVSANGFEGIAFLGRPDLVNLALIAAGVWMQVGF
                     STVIFSAALKSVPVELIEMAHLEGAGNLQIFFKIEIPIIQSTIVLVITQMIMWVLKVF
                     DIIYTLTKGGPFHTSEVVANRMYMTAFSEDNFQYAAAMAVVLFIAVLPVLFINIRNLA
                     YEESVRE"
     gene            complement(2445..3698)
                     /locus_tag="VF531_01750"
     CDS             complement(2445..3698)
                     /locus_tag="VF531_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712720.1"
                     /translation="MKRSRIISLLLVLMLIGMLSLSQLVIAATPTISVTGTWGGSELE
                     TFVKICDAAGVKVNFNTTRDLDAVLATAVKAKTLPDIAILPNPAKLQELAKQGVLKPL
                     TYLSKTDLKNYAKTWISLGSYGGKLYGVYFKVANKSVVWYNPKEFKKNGWAVPKTWDD
                     LIALDKKIVASGKTPWSIGADIGWPLSDWVENIMIRTAGPEVYDKWINHEISWTDPAV
                     KKAFQVWGDIVGDPKNLAGGIDGTLATTFQNGAIAVFQQDPKAYMYYEGDFMGGIVTS
                     ELKSVKLGEDMDFFAFPSIDPKYGVPVVGGADVFVAFSDKPAVKQLMKYLTTVKGNEI
                     AVKNGFLSQNKGVSLDVYPNIVSKNSAKILTSAEVYAFDASDLMKPAVGNQGGFWDAC
                     KKYIQDPSKLDSILAEMEELAKKNY"
     gene            4002..5627
                     /locus_tag="VF531_01755"
     CDS             4002..5627
                     /locus_tag="VF531_01755"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7712721.1"
                     /translation="MFKVMVVDDEPIILDKLHGIIDWEGHGCRIVAETNDSTEALRLA
                     VELQPDIVYSDICMPLMNGIELTEALKQQLPQSVVILISGYDDFNYAQQAIETGVFRY
                     LLKPLNTELFIKVLEEAQQHLTMLEQELQEKEDLKAQIKASLPWLREKFFTDLVNGES
                     SRELSEQQLDFLELDPPEALYNVISVHIDDYASLAGTLKEADFQLSRAHLLNLIQNQL
                     KEATVFLYGFQNKPGELVFVYGIESVSGQDGVFKGLQKAQDSVETMDGLTFSAGLGRL
                     YPGFGSLNISYREALLALEFKLWAGRRAIIPYHDLEKTSVGHLVYHPDYDGFLTMLRE
                     GNLEKTLSFIDQLIASFKSQEYASKSFLHLTVLSLVNQMTRALFEFNISLDTVFGPAY
                     DPFQEINVLETLDDIERWLKDLSRDAITNILRHKQDVSKNFVAKAKLFIENNYQDPEL
                     NLALVAENVYVSSCYLSHLFKESTGSTVIEYLNKIRIRAAKKLLKETQLKIYEIAEQV
                     GFNDYHYFGIVFKKQTGLAPLEYRDKVQFDNLI"
     gene            5865..6893
                     /gene="msrA"
                     /locus_tag="VF531_01760"
     CDS             5865..6893
                     /gene="msrA"
                     /locus_tag="VF531_01760"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7712722.1"
                     /translation="MESTEKGHRQATFAGGCFWCMVSPFKTLDGVIGVTSGYTGGKKP
                     NPTYEEVCSGETGHYEAVRIEYDPSKASYSELLDVFWRQIDPTDPEGQFVDRGTQYCS
                     AIFYHDAEQKQLAESSKAKLEASGRFREPIATRILPAGEFYPAEEYHQDFYCKNPSHY
                     GRYRQGSGRSAYLERTWEKDFVREMQEPNETRDASTNSMELREPHPRTVLTPLQYQVT
                     RENATEPPFHNEYWDNHREGIYVDINSGEPLFSSREKFDSGCGWPSFTQPIEGQSLAE
                     KIDRSHGMIRTEVRSRASDSHLGHVFDDGPTDTGLRYCINSAALRFISREDLEKEGYG
                     EYSKLFKE"
     gene            complement(7096..7752)
                     /locus_tag="VF531_01765"
     CDS             complement(7096..7752)
                     /locus_tag="VF531_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712723.1"
                     /translation="MDVRLTTSLEVSYRVTLEKNDTAKKETTQPDQQTDTKTDSYQHT
                     TSEKKVTYDQPGVKPDWKTIQKLIDESDRANSKLEQIVTELLRRQGLKFKDIYDIQAQ
                     QLQGVEIDETAQKEAQAMLEEGGELSAAKVSDRIVEFAKAISGGDKSKLDQLKKAIDD
                     GFAAAQGVLGRELPEISKQTYEMVMTKLDDWAKEDGTSDKQETPDENHGQNGAVAGSD
                     "
     gene            complement(7931..>8575)
                     /locus_tag="VF531_01770"
     CDS             complement(7931..>8575)
                     /locus_tag="VF531_01770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7712724.1"
                     /translation="QINPHFLYNTLESISWMIAKDKREVAVGLIESLANFFRLGLSGG
                     RDIVTVREELEHVESYLKIQQVRYQDRLDYIIDVDDEVKPERIVKITLQPLVENAIYH
                     GVKPKKDGPGRIYISGSREGDIIRLAVVDNGVGMSRASLAKLQEALTEARLNPETSGK
                     GYSVGNINSRIRLYFGKEYGLHYSSEEGVGTRVEVILPVNWTPASPGEEDGRIE"
BASE COUNT         2192 a         2229 c         2018 g         2136 t
ORIGIN      
        1 agaagatagt taaaggcaaa agcatcgaag cgaaggccgc cggggtcatg atattccagc
       61 ctgaatccag cgaccccaaa aggcttgaga gccttaccgt caacggagtc aaagcgggat
      121 cgcccagtaa gaccagtgcc accagcagat cattccaaat ccaaaggaac tggaatatcg
      181 ccagactggc gaagaccggt ttggacatgg gggtgatcag gatcataaaa atttgaaaat
      241 tgttggcgcc gtcgattttc gccgcttcaa ttaaagaata cggaagttga gcgaaaaagt
      301 tcctgaaaag gtaaatacac agcggcaatc cgaatgccgt atgggctaac cagatgccca
      361 aatatgtccc gttcagctgg agtcctttca acgtcaaaag aaccggaatc agcaccgttt
      421 gcaacggcac gatctgcatc gcgatgatcg cgataaagat cagttttctt cccggaaatt
      481 tataaaagga tatgggatat gcggccagcg ccccgaggaa aatgggcaaa acagtagacg
      541 ggattgcgat aatcagtgaa ttgataaaac ctgttccaat ccccttactg gctaaaacct
      601 cccggtaatt ctccagtgta aaccgttccg gatgcaggaa ggaagtccac catcccgtgg
      661 tcaacatgtc cccaaccggc cggatggaac tggtaaacag catgaatgtt ggaattaacc
      721 aggccagcac aatcacgccc aggattaagt tgatcaatat cggattgccc gataatcggg
      781 cggttgaaac gtcggatttc tgtttcatag atacaatccc ctttccttcc gttattcccg
      841 gacactttct tcgtaggcca ggttgcggat attgatgaat aataccggga gcaccgcaat
      901 gaatagcacc accgccatcg ccgcggcata ttggaaattg tcctcgctaa aagcggtcat
      961 atacatgcgg ttggcaacca cttcactcgt atggaaaggt ccgcccttcg tcaatgtata
     1021 aataatatcg aaaaccttga gcacccacat gatcatctgg gtgatcacca gtacgatggt
     1081 ggattggata atcgggattt ctatcttgaa aaagatctga aggttaccgg ccccctcgag
     1141 gtgggccatt tcaatcagtt caaccggaac cgattttaac gcggcgctga agatcaccgt
     1201 gctgaaaccg acctgcatcc atactccggc cgcaattagc gccaggttga ccagatccgg
     1261 cctgcccaaa aaggcgattc cttcaaatcc attggccgag accaggccga ttttttggag
     1321 caagtggagt gctgcgttga tcacgccatt gatagttccg gtatttacat ctttaaagta
     1381 cataaaattc cagatgactg cagctgctgc tcccgagatg gccatcggca ggaaaataat
     1441 cgtcttcgcc agtgtcgacc accggacccg atccgccagg gcggcaacga gcaagcccaa
     1501 tatgacggtg aaaacagtga aaaataaaag ccagatcaga ttattcctga atgcagtcag
     1561 catggtgcta tccgtaaaca tctgaatata gttttttaac cccgcgaact gctggtcccg
     1621 gccttcactc cgctgggcct gattcttggc tacgattaac atctccgccg tgtctttcac
     1681 ggtcatttcc tcgttgatat cgccgggggc gattttgatc ttatattgga aagcgactct
     1741 attcatcgcc ttccgccaac cgggaatccg ttcaatcaag tcatcgcgtt tgatctccct
     1801 gccgctctcg gtttttaaca cacccagcac atcttgggta aattgactct cgctgaaagt
     1861 caccttctca tggagactaa ggtaaatagt ttgtaatgtc ggcagtacca ggaagaaaaa
     1921 aaccaggatc atcgcaggca gcatccagag ataattcacg gcccattcac gaatatggcc
     1981 tttttccaaa ctctgccccg cattcgattt attcttgcgt ccgatgttca ttcgctcact
     2041 cctttgggta acttgctctc tggcgctcgc cgccagagct gcaactcaaa aaacaattga
     2101 aaaggaaaaa atgacccggg cttgagactc tatattttta tgcgtcgtcc gctttaattt
     2161 caatccttaa tgtaaaatga ttaacaaacc cttaaatcga agtaatcaca caggttactc
     2221 cggtttcaat aatgtatttc cattttcgtg tctttggtgt gtttcgtagt taataaaaca
     2281 ggcttacaga tagaaacttt ttaaccacga aaatcaccaa atacacgaaa tgtctcttct
     2341 ggcgcgatat tttgggataa gaaaagaatt ggaaaaagga ctgccccaaa agtcaatctc
     2401 ggagggctta ttggggcagc ccgcagtatt aacagaatct aaaatcaata attcttctta
     2461 gccagttctt ccatctcggc aaggatggaa tcaagtttgg aaggatcctg aatgtacttt
     2521 ttgcaggcat cccagaaacc accctgattg ccaacggccg gtttcatcag atccgaagcg
     2581 tcgaaagcgt acacttcagc actggtcaaa attttggccg agtttttgct gacgatattc
     2641 ggatagacat ccaaagaaac acctttattt tgagagagga acccgttctt aaccgcgatc
     2701 tcattgcctt tcacggtggt aaggtatttc atcagctgtt tgacggcggg tttatcggag
     2761 aaagctacaa acacgtcggc gccgccgaca acgggaactc catacttggg atcgatggag
     2821 gggaaggcaa agaagtccat gtcctcaccg agcttgacac ttttcagttc ggaggtgaca
     2881 attcctccca taaaatcgcc ttcataatac atataggctt tggggtcctg ctggaagact
     2941 gcgatcgccc cgttttggaa ggttgtcgcc agggtgccgt cgatgccgcc ggccagattc
     3001 ttgggatcac cgacgatgtc gccccaaacc tggaaggctt tctttacggc cggatcggtc
     3061 caggaaatct catggttgat ccatttgtca taaacctcgg gaccggcggt ccggatcatg
     3121 atattctcga cccagtcgga taacggccag ccgatatccg ccccgatgga ccagggggtc
     3181 ttgccggagg ccacgatctt cttgtccagg gcgatcaagt cgtcccaggt cttggggacc
     3241 gcccaaccgt tcttcttgaa ttccttcgga ttgtaccaga caaccgattt attggcgact
     3301 ttgaaataca cgccgtacag ttttccgccg tagctgccga ggctgatcca ggttttggcg
     3361 taattcttca ggtcggtctt gctcaggtag gtaaggggtt tgagcacgcc ctgcttcgcc
     3421 agctcctgca acttggcggg attcggcaga atcgcaatat cgggaagggt tttggctttg
     3481 accgccgtag ccaaaacagc gtccagatcc cgggtggtat tgaaattcac cttcacaccc
     3541 gcggcatcgc agatcttcac aaaggtttcc aattcacttc cgccccaggt tccggtgacg
     3601 gagatggtgg gggttgccgc gattaccagt tgcgacaggg agagcatccc gatgagcatc
     3661 aataccagca gaagactgat gattcttgat cgcttcactt tcacacgctc cttatccttt
     3721 tttagatggt ttcattccca tttcacccgt ggtggtttag tcatgggacg ctaccatcca
     3781 cttcgatgtt accaagattg ggccggattc aaaagtcaaa atctttttat tttggtcgtt
     3841 atcttgtgat ccgttgatag gggtaacatt ttttggaatg gaatgcaata tcaacctatc
     3901 attgaataaa caactgtccc ggtcgcatcc attgtattct tggttatttt cgtgtaaatt
     3961 agtgtagagt gatgccaaca acgccagtga ggagatagtt catgttcaag gtgatggtgg
     4021 tggacgatga gccgattatt cttgataagc ttcacgggat catcgactgg gagggtcacg
     4081 gatgccggat cgttgcggag accaatgaca gcaccgaggc tctgcggctg gcagtggaat
     4141 tacagccgga catcgtttat tcggacatct gcatgcccct gatgaacggg atcgaactca
     4201 ccgaggcgct caaacagcag ctgccgcaga gcgtggtcat cctgatcagc ggctacgacg
     4261 attttaacta cgcccagcaa gccattgaaa ccggagtctt ccgttatctt ttaaagccgt
     4321 taaacaccga attgttcatc aaggtactcg aagaggccca acaacacctg accatgctgg
     4381 aacaggagtt gcaggagaaa gaggatctca aggcacagat taaggcgagt ctaccctggt
     4441 tgcgggagaa atttttcacc gatctggtca acggcgagtc atcccgggaa ctctccgagc
     4501 agcagctgga cttcttagag cttgacccgc ccgaggcgct ttataacgtg atcagtgttc
     4561 atatcgacga ttacgcctcc ttggccggga cgcttaagga agcggatttt caattgagcc
     4621 gggcccatct gctgaacctc attcagaacc agctcaaaga ggcgacggtt tttttatacg
     4681 ggtttcaaaa caaacccggg gaattggtgt ttgtctacgg aatcgaatct gtaagtggac
     4741 aggatggggt tttcaaaggt ctccaaaaag cccaggatag cgtggagacc atggacggat
     4801 tgacgttctc cgccggcctg ggccgtttat atccgggttt cggttcgcta aacatctcgt
     4861 atcgcgaggc gttactggcc ttagaattca agctctgggc cggacgaagg gcaatcatcc
     4921 cctatcacga cctggagaag accagcgtcg gccatctggt ttaccatcct gattatgatg
     4981 gtttcctaac catgctgcgg gaggggaacc tggagaagac cctgagtttt atcgatcagc
     5041 tcatcgcctc ttttaaatct caggaatacg catccaagag tttcctgcac ttaaccgtgc
     5101 tcagtctggt gaatcaaatg acccgggcac tgtttgagtt caacatctcc ctcgacaccg
     5161 tcttcggccc ggcctatgat ccgtttcagg agatcaacgt cctggaaacg ttggacgata
     5221 ttgagcgctg gttgaaggat ttgtcccgag acgccatcac caatatcctc cgccataagc
     5281 aggatgtgag taagaacttc gtggccaagg cgaagctttt cattgagaac aattatcaag
     5341 acccggagct caatttagcc ctggtggcag agaatgtcta tgtgagctcc tgctatctga
     5401 gccatctttt caaggaaagc accggaagca cagtaatcga atatctcaac aagatccgga
     5461 tccgcgccgc caaaaaatta ctcaaggaaa cccagctgaa gatctacgag atcgcggaac
     5521 aggtgggttt taacgactat cattacttcg ggatcgtctt caagaaacag accggtctgg
     5581 ctccgcttga ataccgcgat aaggtgcagt tcgataattt gatttaggcc tatccccttt
     5641 ccagctattc attacccata agtaacatta atgactataa aaatacgaat tccccgagat
     5701 tattggtgta taatttgttt ggatatttgt attacaactc taatttaaag ataaggtaat
     5761 cagcctaaga ctaaaccata ctctatccag gcatattaac aatgcccgca ctcatcaact
     5821 cccaactact tgagtgattt taacattcac cacaggagac ttttatggaa tcaactgaaa
     5881 aaggtcatcg ccaagccaca tttgccgggg gctgtttttg gtgcatggta tcccccttta
     5941 aaaccctgga cggagtcatc ggggtcacca gcggctatac cgggggaaaa aagccaaatc
     6001 ccacttatga ggaggtctgt tccggagaga ccggccatta cgaggcggtg agaatcgagt
     6061 acgacccttc aaaagccagc tactccgagc tgcttgacgt tttctggcgg cagatcgatc
     6121 ccaccgatcc cgaagggcag ttcgtggata gggggacgca atactgcagc gccatctttt
     6181 atcacgacgc tgagcaaaag caactggccg aaagctccaa agcaaagctg gaagcctccg
     6241 gcaggtttag ggaacccatc gccacccgga ttctcccggc cggagaattt taccccgccg
     6301 aagaatatca tcaggacttt tactgtaaga acccctccca ttacgggcgt taccgtcagg
     6361 gttcggggcg gtccgcctac ctggagcgga cttgggagaa agacttcgtc cgggagatgc
     6421 aagaaccaaa tgaaaccagg gatgcttcta ccaactcgat ggaactaaga gagccgcatc
     6481 ctcgcacggt attgaccccc cttcagtacc aagttacccg ggaaaatgcc accgagcctc
     6541 cttttcataa cgaatactgg gacaatcacc gcgaggggat ttatgtggat atcaactccg
     6601 gggagccgct cttttcctcc cgggaaaaat tcgactccgg ctgcggttgg ccaagtttta
     6661 ctcaacccat tgagggtcaa agcttagcag agaaaataga ccggagccac gggatgatcc
     6721 ggaccgaggt ccgcagccgc gcgagtgatt cccatctcgg ccacgttttt gacgacggcc
     6781 ccaccgatac cggcctgcgc tactgtatca actccgcggc gctgagattc atctcccggg
     6841 aagacttgga aaaagaaggg tacggggaat attcgaagtt gttcaaggag taataaacaa
     6901 agtgtgaagt ttgaagtgtg aaacaaaatc taaaggcttt aacttcatag gcttaattca
     6961 aacttttaaa gcccccggag ccggaacatc gcaaacgaca ttacgtcatt cttgacgtgt
     7021 cacatctttg ggggctttaa aaataccgca tttgtccttc atacttgatc aacatctcta
     7081 aaacagaaaa caaacttaat cggaaccggc gacggcccca ttctggccgt ggttttcatc
     7141 cggggtttcc tgcttatctg aggttccatc ttccttagcc cagtcatcca acttcgtcat
     7201 gaccatctca taggtttgct ttgaaatctc cggaagttcc cggcccagta ctccctgggc
     7261 cgccgcgaac ccgtcatcga tggccttctt cagctgatcc agtttcgatt tatctccccc
     7321 ggaaatcgcc ttggcgaact cgacgattct gtcgctaacc ttggcggcgc tgagctcccc
     7381 gccctcctcc aacatggcct gggcttcctt ttgggcggtt tcgtcgatct cgacgccttg
     7441 caattgttga gcctgtatat catagatgtc cttaaatttc agcccctgcc gccgcaacaa
     7501 ctcggtaacg atttgttcca atttggaatt ggccctgtca ctctcatcaa tcagcttctg
     7561 gatggttttc caatccggct tgactccggg ctgatcataa gtcacctttt tctccgaagt
     7621 agtatgctga taggaatcgg ttttcgtatc cgtttgttga tcgggttggg tcgtttcctt
     7681 tttggccgta tcgtttttct ccagggttac acgatacgat acctccagac tggtagtcaa
     7741 tcgaacatcc atgtttatcg acctccatgt gtaaattgtg aaatgtgaag ggtaaagggt
     7801 gaagaaaaat ccgaacattt tataccccgg ccttcgcaat tcaaactttt gtcatctatt
     7861 acataatata tcggcaaacg gtttcggtcg caagatagtc caaacgactc attttgcccg
     7921 tcgcgcggcg ctactcgatc cgcccatcct cctcccccgg actggctgga gtccaattca
     7981 ccgggaggat cacttccacc cgggtgccga ccccttcctc gctggaataa tgcagcccat
     8041 actccttgcc aaaataaagc ctaatccggg aattgatatt cccgacacta tatcccttgc
     8101 ccgaagtctc cgggttcaat cgcgcctcag tcaacgcctc ttgcagtttg gccaggctcg
     8161 cccggctcat ccccacgccg ttgtcgacca ccgccaaccg gataatatcc ccttccctgc
     8221 taccggagat ataaatccgg cccggaccat cttttttggg tttaaccccg tgataaatcg
     8281 cattttcaac caatggctgt aaggtaatct taacaattcg ttccggtttg acctcatcat
     8341 ccacatcaat gatatagtca agccggtcct gataccgcac ctgttgaatc ttcaagtaac
     8401 tctccacatg ctcgagttcc tcccgaaccg tgacaatatc ccgcccgccg cttaaaccca
     8461 aacggaaaaa attagccagc gattcaatca aacctacggc aacctcacgc ttgtccttgg
     8521 cgatcatcca ggagatagat tccaatgtat tgtagagaaa atgcgggtta atctg
//
LOCUS       DASVMB010000051         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_79626,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000051 DASVMB010000000
VERSION     DASVMB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79626"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VF531_01775"
     CDS             complement(<1..642)
                     /locus_tag="VF531_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7712725.1"
                     /translation="MFPEERRHIITEILRKQGRCRVDDLAGKLQVSEVTVRQDLDILE
                     REGMLRRTHGGAILNPQSSFERSFQLEATSFAREKERIGQAAAALVEDGATIILDVGT
                     TVTQLARNLTERKDLTVFTNALNIAMLLEDAPGVELIVTGGTLRRQQHSLVNPFGSLI
                     TERINADMVFIGCNGIDAEHGLTNVNLAEAEMKTLFIKAARHRVVLADSSKVGN"
     gene            892..2244
                     /locus_tag="VF531_01780"
     CDS             892..2244
                     /locus_tag="VF531_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7712726.1"
                     /translation="MKIPKAATADKERLYRRNACLSIGDAMFFMIMTGLVTPFQGVYL
                     LGLGGTNFQVAWLNALPPLVTALCVLPATRYISARNEHYLPVVLASSAINRLFYLIYG
                     LIPFVVVWPSLRPLVFILLFGLATIPGTYSNLAWTSMIGRLIPRSRRGRFFGLRNTLS
                     VAISMIATLFGGLMLDHYPFPINFTYIFIIAFGGVLCSLALLAFTVEYDAEQELETVS
                     ATPRGNGGEPAQLQPIHYASHDLTRFYLATFLVHLGINMIGPILTIYHVRILKLDNTV
                     IGLLATVSSLASVATYVIWGRLSDRIGNLKTMIGVIVGLVVFSAAYLVDRQLWFLILM
                     NLLNGAFTAGFGLIIFNLLLEISPPQERSNTIAYFNLNANLSSFIAPLLGTALVLGNN
                     LWFPFILAALIRIAAMVVFWMIGRPSPGWMEYLKIHWMESRISISNRLLNRKRRNFRA
                     "
     gene            complement(2234..>2722)
                     /locus_tag="VF531_01785"
     CDS             complement(2234..>2722)
                     /locus_tag="VF531_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712727.1"
                     /translation="ELLLGHKITKIVSDLLNFEDRTVLPYDELLWAREPVGDPMLAAA
                     GLAVTSDGFLEVTSTLQSVTAPFIFGSGLGVTAGAGRGAGIAAVVSRERARLLYENME
                     RWLNEAPLKTVKSSGQAASEIIPLGGGEGILQTPEFVTRGSIPWKWKQRMEQKNLKRL
                     RP"
BASE COUNT          619 a          725 c          630 g          748 t
ORIGIN      
        1 atttcctacc ttgcttgaat ccgcgagtac cacccggtgt cgagccgcct tgataaacaa
       61 ggttttcatc tcggcttctg ctaagttgac gttggtaagg ccatgttcgg catcaatccc
      121 gttacagccg atgaaaacca tatcggcgtt gattcgttcc gtaatcaagc tgccaaaagg
      181 gttgaccagg gagtgctgtt gacggcggag agttccaccg gttactatca gctccacacc
      241 cggggcgtct tccagcagca tggcgatatt tagcgcattg gtaaaaacag taagatcctt
      301 ccgttcggta aggttacggg ctaattgggt aacagtggtc cccacgtcca aaatgatcgt
      361 cgctccgtct tcgactaaag ctgcggctgc ttgaccgatc cgttctttct cgcgtgcgaa
      421 ggaagttgcc tccagttgaa aagagcgctc gaaactgctt tgtgggttta agattgcccc
      481 gccatgagtt cgccgcagca ttccttctct ttccaatata tctaaatctt gcctgacggt
      541 aacttcagaa acctggagtt taccggctaa atcatctacc cggcaacggc cttgtttgcg
      601 aaggatctct gtaatgatat ggcgtcgttc ctcgggaaac accgggattc cccctttctg
      661 ccaatcctaa cctattctaa ttttacgttt aatttttttt ggtgtcaatt attttcttta
      721 ttgggaaggc aaatgaactt ccgtaagcaa acgaaagatc attgccgaaa aaatccccta
      781 taagtaggtg acaaaacact tgcttttttg atgaaatcgt cagacaatga taatagaaat
      841 accggcaatg acaaacgcgt ttaaaatatc cgaaacggga gcttcggttc gatgaaaata
      901 ccaaaagccg ccactgccga taaagaacgg ttataccgcc gaaatgcttg cttaagcatc
      961 ggtgatgcca tgttttttat gattatgacc gggctcgtca ctcctttcca gggagtttat
     1021 ctcctggggc tgggtggaac caatttccag gtggcgtggc taaacgcact accgccattg
     1081 gtaactgccc tttgtgtctt gcccgccacc cgctatatct cggcccggaa tgaacactac
     1141 ctaccggtgg ttctggcaag cagcgcaatt aatcgtttgt tttacttgat atacgggtta
     1201 attccattcg tggttgtctg gccaagtctg cggccgctgg ttttcatttt gctcttcggt
     1261 ctggccacca taccggggac ttactccaac ctcgcctgga cctcaatgat cggccggctg
     1321 atccctcgtt cacgacgggg ccggttcttt ggcttgcgaa atacgctgtc cgtggcgata
     1381 tccatgatcg ccaccctatt cggcggcttg atgcttgacc attacccgtt tcccattaat
     1441 tttacctata tattcatcat cgctttcggg ggggtactgt gttctctggc cttactggcc
     1501 tttaccgtgg agtacgatgc cgagcaggaa cttgagacag tttcggctac tcccagagga
     1561 aacggtggag aaccggcaca actgcaaccg atccattatg cgtcccatga tcttacccgg
     1621 ttctatctgg ccaccttcct ggttcacttg ggaattaata tgatcggacc gattctcacc
     1681 atctatcacg tacgaatcct caaactggat aataccgtca tcggactgct cgccaccgtc
     1741 agttctctgg cttctgtggc gacttacgta atctgggggc gactttccga ccgaattggc
     1801 aatctgaaaa caatgatcgg cgtcatcgtg ggtttggtgg tattttccgc ggcatatctg
     1861 gtcgataggc aactttggtt tcttatactc atgaatttac tcaatggcgc ctttaccgcc
     1921 ggatttggac tgatcatctt caacctgctt ttagagatct ctcctccgca ggaacggagc
     1981 aacaccattg catatttcaa cctgaacgcc aacctctcct cgtttattgc gccactattg
     2041 ggaacggctt tagttctcgg aaacaacctc tggttccctt ttatactggc cgccctgatc
     2101 cggatagcgg cgatggtggt gttttggatg attggccggc caagccccgg ttggatggag
     2161 tatctgaaaa tccattggat ggaaagccgg atcagtatca gcaaccggtt gttgaaccgt
     2221 aaaagaagaa atttcagggc ctgagccgct tcaaattttt ttgttccatc cgctgcttcc
     2281 atttccaggg gatactgccc cgggttacaa actcgggtgt ttggaggatg ccttcaccgc
     2341 cacccaaggg aatgatctcc gatgcggctt gccccgaaga ttttacggtc tttaacggtg
     2401 cttcgttcaa ccaccgttcc atattctcat ataataatcg ggcacgttcc cttgaaacca
     2461 ccgcggctat tcccgcaccc cggccggctc ccgcggtcac tcccaacccg ctcccgaaga
     2521 tgaacggcgc agtcacggat tgcagggtgg acgtaacctc taaaaaaccg tcgctggtca
     2581 cagccaaacc cgcagctgca agcattggat ctcctacagg ttctctggcc cataataatt
     2641 catcgtaggg taacaccgtt cgatcctcaa aattgagtaa atccgaaaca atcttggtta
     2701 ttttatgtcc caataacaat tc
//
LOCUS       DASVMB010000052         6372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_79714,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000052 DASVMB010000000
VERSION     DASVMB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79714"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="rsgA"
                     /locus_tag="VF531_01790"
     CDS             <1..966
                     /gene="rsgA"
                     /locus_tag="VF531_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX7712728.1"
                     /translation="DYGQKLRVTVENGEVLAERPVRENFGVEQLAVGDWVVLEHSFDT
                     VQIRKVLERQTKFSRAAAGIEVKEQIVAANVDIVFLMQSLNRDFNLRRLERYLIAAWE
                     SGAIPVIVLTKADCCEDVAAKVSQVYEIAPGVEVHAISAVTGKGMVELRHHLKPGKTI
                     ALLGSSGIGKSTLVNTLAGQEVLKTQAIRENDNRGRHTTTHREIVLLPEGGLILDTPG
                     MRTLALWEADAGMEVMFGDIEQLIAACRFSDCGHGNEPGCAIREALQSGELDPKRWES
                     WQKLQRELAYLEAKKDGQVRQMQKEWGKQIAKFQKDLHKGAYRDE"
     gene            959..1957
                     /locus_tag="VF531_01795"
     CDS             959..1957
                     /locus_tag="VF531_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7712729.1"
                     /translation="MNDVQKITDQIVTHDLTKVYRTFQKEAGFKGSIQSLIRRQTVEK
                     TAIRKFDLSIDKGEFVGLIGPNGAGKTTLIKMLTGIIHPTEGEVTVFGFTPYQLKDDF
                     KKNFAVVMGQKSQLWWDLPAEDSFLLNKALYGIPDPEYRQSLGFFSELLGISGLVKIP
                     VRNLSLGERMKMELVLALLHNPRVLFLDEPTIGMDAVAQKQIRQFLKEINEAKGVTII
                     LTSHYMEDIKHLCKRTIVVRNGIKIYDGDLAALLDKYRNYRIISISFETAADLNIEMP
                     VEWLEKGPYKAVFKVPKEEAKAVLYHIMANQEVEDIRIEDEDLGGVVEKIYTAEVR"
     gene            1963..2757
                     /locus_tag="VF531_01800"
     CDS             1963..2757
                     /locus_tag="VF531_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX7712730.1"
                     /translation="MRKYLEIAKITFKLQIAYRFDVITGVLFSFARILLAFILWSVMF
                     RGKDTIAGFSFAAMMTYYIIMSFFRRLDLTDGIVWQLSAEIREGQFSKYLVRPVRPLG
                     YFVASALSKTAFVFGINLLATIGYGFIFRHYFLIRVSPVICLNAVLIWLLGLNFMILL
                     SYFIAVLSFKFIDVGALNMIKGTILEFLTGALIPLAILPGWLQEGMRLFPFYYVYYYP
                     TMLFLNDQTGQIPAAILIMILWNGLMLAVISVGYGMLRRRYEGVGA"
     gene            2754..3551
                     /locus_tag="VF531_01805"
     CDS             2754..3551
                     /locus_tag="VF531_01805"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX7712731.1"
                     /translation="MKLLRYFKFCGMLLKFKLSRQMVYGFNFWAAFFVDLTVFLIQIA
                     VFSAIFLQVDTINGWNRYQMIFFVGTFTIIDSLYMATYFFGVIGIPEKVQSGKLDIYL
                     TKPVNPLFLVSFENMDFGSLLLTIPGMMMIGYSVVNLGITLSLPKIIGYLALLIMMLI
                     LMYDLMVLVRSAAFWFTSINSLQEFENEMVYFSFRIPGVVFKGFTKLVFYIILPYGLM
                     ATIPTQFFTGLLDMKGWLMTIAVCGVFTYISQFVWRKGLARYASASS"
     gene            3741..4415
                     /locus_tag="VF531_01810"
     CDS             3741..4415
                     /locus_tag="VF531_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7712732.1"
                     /translation="MIKLSVMGDQIELDEYTDDVTIDDLAKAVDSFRLHHLQSQGLKT
                     RNCCGCGICCHDLIPVLGMDLAAFGYQPGRGDEPLRNQLVLPRHPDIRQRSKDIRETM
                     AQFGISEPEATFLYDVNQAEPLALPRRDDGGCRYLQGNLCGIYEKRPFNCRFYLCNMG
                     TRLAAIQETLVSQGTWHAYAMLGWIPAKALRYNPFMRTQDYSAISLADLEFNLDKNVE
                     KMFFYF"
     gene            complement(4485..5591)
                     /locus_tag="VF531_01815"
     CDS             complement(4485..5591)
                     /locus_tag="VF531_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7712733.1"
                     /translation="MKHLQRVVLGIILISLSGCWGQVDIEKVGPILQLGVEAAPKGRI
                     IVTHSFPVFGAASKKQDELVSMQTDNLRESREIWRRISPQIPQGGKVQQYLFAADFAR
                     QGITPWMEVFQRDPINPPLISVVIVDGSPKELMEKAANFKDKPRTALYITQLLDNNNN
                     QYAAVDDIFRFTIAYFAPGLDPVAPIIKLEPKDIRLTGTALFHGDRMVGSINIDQTIM
                     LLALMGRLREAEYIFRHPAPLPGMGPGKPAAAARLTVAKRKIEIRLIHGRPVVEVNLL
                     FSGVLDEYRWDQMDRSGAQHKLEQSFAKELTGLCNHLIGRLQQVGSDPIGIGDRIRAK
                     FYRYWLRNKPGQIYQATQFKVKVKMHLTKYGAIK"
     gene            complement(5588..>6372)
                     /locus_tag="VF531_01820"
     CDS             complement(5588..>6372)
                     /locus_tag="VF531_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX7712734.1"
                     /translation="FLRILSEYIQLFQLVRIPSIIVTILIMLPTLFLTRKGYWAIGRF
                     SYFLVVILGAFVLLGLKAGADIRLSFLQPVGSAGWPKLLKTIPTIFFAFIGFELSAFI
                     YKDVKERRQATFWALSATIITLLFLELVCLCTTGVFGETMLKQMVAPLFNLSQFVRFP
                     FFERMDLFFFILWFPLLETTFRVYFATAYTGIYKLTRLNDSLLYYALFSLIVFGLSLL
                     PTDLNQMMQLGMVVNLAGTAVILYLVFCWGFSLLRRQGGTNK"
BASE COUNT         1658 a         1390 c         1628 g         1696 t
ORIGIN      
        1 gattatggac agaaactacg tgtaacggtt gaaaacggtg aggttctggc cgaaaggccg
       61 gttcgtgaga attttggagt agaacagttg gccgtaggcg attgggtggt tttggagcat
      121 tcctttgata cagtgcaaat tcggaaggtt cttgagcggc aaaccaaatt ttcgagggct
      181 gcggccggga ttgaagtgaa ggaacagatc gttgcggcca atgtggatat cgtttttctg
      241 atgcaatcac taaaccggga tttcaatctg cggcggcttg aaaggtatct catagccgcc
      301 tgggaaagcg gggcgattcc ggtcattgtg cttaccaaag ctgactgctg tgaggatgtg
      361 gctgcaaaag tcagtcaagt atacgagata gccccgggag tcgaggtaca cgcgattagt
      421 gctgttactg gcaaaggtat ggttgaattg cgccaccatc ttaagccagg aaagaccatc
      481 gcccttttgg gttcgtccgg tattggaaag tccacgctag tcaataccct ggccggacag
      541 gaggttttga aaactcaggc aatccgggaa aacgataatc ggggcagaca tactaccact
      601 catcgcgaga ttgtactctt acctgaagga gggttgatcc tagatacccc tggtatgagg
      661 actctggcgc tttgggaggc ggatgcaggc atggaggtca tgttcggcga tattgagcaa
      721 ctgatcgcag cctgccgttt cagtgattgc gggcatggca atgaaccggg ttgcgcgatc
      781 agagaagcat tgcaaagcgg cgaactggac ccgaaacgct gggaaagctg gcaaaaactg
      841 cagcgggaat tggcgtatct ggaagctaag aaagatggcc aagtgagaca aatgcaaaaa
      901 gaatggggca aacaaatagc taagtttcaa aaagatttgc acaaaggagc atatcgtgat
      961 gaatgatgtt caaaagataa cggatcaaat tgtcacccac gacctaacca aggtttatcg
     1021 cactttccaa aaagaagccg gtttcaaggg aagcattcag tctttgattc gcaggcaaac
     1081 cgttgaaaag acggcgatac gcaaatttga cttgagcatc gataaaggtg agtttgtggg
     1141 gctgatcggc ccgaacggcg cggggaagac tacgctcatc aaaatgctca ccggaatcat
     1201 tcacccaacg gaaggagaag tgacagtctt tggtttcact ccttaccaat tgaaggatga
     1261 cttcaaaaag aattttgcgg tggtgatggg gcaaaaaagc cagttgtggt gggatctgcc
     1321 ggcagaggat tcttttctat tgaacaaagc gttatacggg attcccgatc cggagtatcg
     1381 ccaaagtctc ggttttttta gcgagttgtt gggaatcagc gggctagtca agatcccggt
     1441 gcggaatctc tccctgggtg aaagaatgaa gatggagctg gttttggcat tattacataa
     1501 cccccgggtc ctgtttctgg atgaacccac catcgggatg gatgcggtgg ctcagaaaca
     1561 gatccgccaa tttttaaagg agatcaatga agccaagggc gtaaccatca ttctcaccag
     1621 ccactatatg gaggatatca agcatctgtg caagcgaacc attgtggtca gaaacgggat
     1681 caaaatttat gatggggatc tggcggcgct ccttgataaa tatcgcaatt accgcattat
     1741 ttcaatctca tttgagactg cggcggactt gaacattgaa atgccggtgg aatggcttga
     1801 aaaagggcct tataaagcgg tttttaaggt tccaaaggaa gaggccaagg cggtccttta
     1861 tcacatcatg gcaaatcagg aagttgagga tatccggatc gaggatgaag atctcggcgg
     1921 cgtggttgag aagatatata ccgccgaggt gaggtaatga agatgagaaa atatttggaa
     1981 atcgccaaaa tcacgtttaa attgcagatc gcttatcgct ttgatgtcat caccggggtg
     2041 ctgttctcgt tcgcccggat attattggcc tttatcttat ggagtgtcat gtttcgaggg
     2101 aaggatacca tcgccggttt ttcattcgcg gccatgatga cctactacat catcatgtcg
     2161 ttcttcagaa ggctcgatct gacggacggg attgtctggc aattgtccgc agagatacgg
     2221 gaagggcaat tcagtaaata tttggtcagg ccggtcaggc cgctgggata ctttgtggcc
     2281 tcggctcttt ccaagactgc ttttgttttc gggattaatc tcttggctac cattgggtat
     2341 ggattcatct ttcgtcacta ttttttgatc cgggtaagtc cggtgatctg tttgaacgcc
     2401 gttttgattt ggctattggg gttgaacttc atgattctat taagttactt tatcgcggtt
     2461 ttgagcttta aattcattga tgtcggagcg ttaaacatga tcaaagggac tattttggag
     2521 tttttaaccg gcgcgttgat tcctctcgcc atcctgcccg gttggctgca ggaagggatg
     2581 aggctcttcc ccttttatta cgtgtattac tatccgacca tgttgttttt gaacgatcaa
     2641 accggacaga ttcctgccgc tattctgata atgatcttgt ggaacgggtt gatgttggcg
     2701 gttatatccg tggggtacgg aatgctgcgg cgaaggtacg agggggtggg ggcatgaagt
     2761 tgctgcgcta tttcaagttc tgcggcatgc tattgaagtt caaattaagc cgccaaatgg
     2821 tatatggctt taatttttgg gctgctttct ttgtggatct gaccgttttc ttgatacaga
     2881 tagccgtctt ctccgccatt tttctgcaag tagacactat caacggctgg aacaggtatc
     2941 agatgatctt tttcgtcggt actttcacca ttatcgacag tctttatatg gcaacgtact
     3001 tctttggggt gattgggatt cctgaaaagg tgcaatccgg taaactggat atctatctca
     3061 ccaaaccggt taatccgctt tttctggtct cttttgaaaa tatggacttt ggatcactgc
     3121 tgcttacgat cccgggaatg atgatgatag gctatagcgt cgtgaatctg ggaatcactc
     3181 taagcctgcc gaagatcatc gggtatctgg cactacttat catgatgttg atcttgatgt
     3241 atgacttgat ggtgctcgtt cggtccgccg ctttttggtt taccagtatc aattctttgc
     3301 aagagtttga aaacgagatg gtatatttca gtttccgcat tccgggagtg gtttttaaag
     3361 gcttcacgaa attggtgttt tatatcatct tgccgtacgg gctgatggct accatcccta
     3421 cgcagttctt caccgggctt ttggatatga aaggttggtt gatgaccatc gccgtatgcg
     3481 gggtttttac atacataagt caatttgtat ggagaaaggg actggcacgt tatgccagcg
     3541 cgagcagtta agacattaca tggctaatca tttagctcaa ggagaattgc agtatatcca
     3601 ggtactgtta cgaaaccttg aacattttca cttctaagtg gactattgtc ccaaaatgga
     3661 ctataattcc gcctaataca gggaaaagat attgttgtat gcacgcgtgc gtacacatac
     3721 ccgcggcgga gatgaatatc gtgattaagc tatcggtgat gggtgatcag atcgaactgg
     3781 atgagtatac agatgatgtt acgattgatg atttggcgaa ggcagtcgat tcctttcgcc
     3841 tgcatcatct ccagtcccaa ggattgaaaa cgcggaactg ctgtggttgc gggatctgct
     3901 gccatgatct tattccggtg ttgggaatgg atttggctgc cttcggttac caaccggggc
     3961 gaggtgacga accgctccgc aaccaactgg tgctgccaag gcatcccgac atacggcaac
     4021 ggtccaaaga tatccgggag accatggctc aatttgggat cagtgaaccg gaagccacat
     4081 tcttatatga tgtcaaccag gcggaaccat tggctttacc caggcgggac gacggcggct
     4141 gccggtatct gcagggaaac ctctgtggta tttatgagaa acggccgttt aactgccgtt
     4201 tttatctctg taatatgggg acgcgtttgg cagctatcca agagactttg gtcagtcaag
     4261 gcacttggca cgcgtacgcc atgctgggat ggataccagc aaaggcgtta cggtataatc
     4321 cttttatgcg cactcaggac tatagcgcca tttcattagc ggatctcgaa tttaatttgg
     4381 ataaaaatgt agagaaaatg tttttctatt tttgatgtga aaagacgtac ttgatcttct
     4441 cctgtaaatt atatttgcat aaattactac ttggtgtgtt gggattattt aatcgcccca
     4501 tatttggtaa ggtgcatttt gaccttaact ttaaactggg ttgcctgata aatctgcccc
     4561 ggcttattcc gcagccaata ccggtaaaat ttagcgcgga tccgatcacc gatgcctatc
     4621 gggtcgctcc ccacctgctg taaacggccg attaggtggt tacagagccc ggttaactct
     4681 ttcgcaaaac tttgttccaa tttatgttga gctccggagc gatccatctg atcccaacga
     4741 tattcgtcca aaacacccga gaaaagcaga ttaacctcca ccaccggacg cccgtgaatc
     4801 agccgtattt cgattttgcg tttggcgacg gtgagccgcg ccgcggcggc cggtttgccg
     4861 ggccccattc caggcaatgg ggccggatga cggaagatat attcggcttc ccgtagccgc
     4921 cccattaatg ctaaaagcat gatggtctgg tcgatattaa tcgaaccgac cattcgatcc
     4981 ccgtgaaaca aagcggtgcc ggtcagccgg atatccttgg gttccaattt gatgatcggg
     5041 gcgaccggat ccagtcccgg cgcaaaataa gctatggtaa atcgaaaaat gtcatcgacc
     5101 gccgcatact gattgttgtt attatccaac aattgcgtaa tataaagcgc ggtacggggc
     5161 ttatctttaa aatttgccgc tttctccatc agttctttcg ggctgccgtc cacaatcacg
     5221 accgaaatga gggggggatt aatcggatct ctctgaaaaa cttccatcca aggcgtgatc
     5281 ccttgccggg caaaatccgc tgcaaacaga tattgctgca ctttcccgcc ttggggaatt
     5341 tggggtgaaa ttcttcgcca gatctcgcgg gattcccgga gattatccgt ctgcatggat
     5401 accagttcat cttgtttttt actggctgcc ccaaataccg gaaaactatg ggtaactata
     5461 atccgcccct taggtgcggc ttctactccc agttgcagga ttggtccgac tttttcgatg
     5521 tcgacctgtc cccagcatcc gcttagcgaa attaggataa tccctaaaac tacccgctgt
     5581 aaatgtttca tttatttgtg ccgccctgcc ggcgcagtaa ggagaatccc caacaaaaca
     5641 cgagatataa aatgacggcc gtcccggcga gattcaccac catccccagc tgcatcatct
     5701 ggttaaggtc ggttggtagc aggcttaagc cgaaaacaat caaactgaac aaggcataat
     5761 aaagaagaga atcattcaag cgggtcaact tatagattcc agtataggct gtggcgaagt
     5821 aaacccggaa agtggtttcc agcaagggaa accataaaat aaaaaagaat agatccatcc
     5881 gttcaaagaa agggaatctg acaaattggg agagattaaa gagtggcgcg accatctgct
     5941 tcagcatcgt ctctccgaaa accccggtcg tgcaaagaca gaccagttcc aaaaaaagta
     6001 aagttatgat cgttgcactt aatgcccaaa acgttgcctg tctccgttcc tttacatctt
     6061 tgtaaatgaa ggcacttaat tcaaatccga taaaagcaaa aaatatagtc ggtatcgtct
     6121 tcagtaattt cggccacccc gcgctaccca ccggttgcaa aaaacttaac cgtatatcgg
     6181 cgccggcctt caaccctaag agtacaaacg ctcctaaaat caccacgaga aaataggaaa
     6241 aacggccgat tgcccagtac cctttacggg ttaaaaagag cgtcggcagc ataatcagta
     6301 tggttacaat aatggacggt atccgaacca attggaacag ctggatgtat tcggaaagta
     6361 tccttaggaa aa
//
LOCUS       DASVMB010000053         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_373055,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000053 DASVMB010000000
VERSION     DASVMB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373055"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1127
                     /gene="pfkA"
                     /locus_tag="VF531_01825"
     CDS             138..1127
                     /gene="pfkA"
                     /locus_tag="VF531_01825"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX7712735.1"
                     /translation="MMRIGVLTSGGDAPGMNAAIRSIVRTGLYHGCEMYGIKKGFSGI
                     LSGEIIPMDSLSVADILQRGGTILQSARCLEFKTPEGQAKAKQQLENLGLNGLVIIGG
                     DGSFHGAQVLNKMGFQTVGIPGTIDNDIGCTDYSIGFDTAVNTVLDAMNKIRDTASSH
                     NRVCVIEVMGKNAGFIAVAAGLAGGAEAVLIPEVPYNLEELSSQIIQNNVRGKVHSII
                     LVAEGLFGDPTSLDHSSAFKVGNYLSLRTGADTRVTILGHLQRGGTPSYFDRKLGTLM
                     GAEAVELILAGETEKMVGFTQNRIQVYDLDTAIKTKKTVDPDELELVNVMANI"
     gene            1276..2694
                     /gene="pyk"
                     /locus_tag="VF531_01830"
     CDS             1276..2694
                     /gene="pyk"
                     /locus_tag="VF531_01830"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7712736.1"
                     /translation="MKKTKVICTLGPSSSDHAVLEKMINAGMNIARLNFSHGTYEEHG
                     ARIKLVRDVASSLGKPVGILADIRGPKIRTGILSEEPLILSDGSRIRLSIDQGKEQTP
                     GYIYVDYPSLLQDLHPGSKIFLADGMIELQAVQIGSNDVECEVTSGGELTSRKGVTLP
                     GVSVNLPALMDKDRRDIAFLVEQKIDFIAVSFARKAEHIVEIRKLLQDLGGDQLLIAK
                     IENEEGLHNVDELIKVCDGIMVARGDLGVEVPLEEVPLIQKYLIQRCNLAGKPVITAT
                     EMLESMVRNPRPTRAEITDIAHAITDGTDAIMLSAETAVGKYPVAAVEIMTRVAEKIE
                     GSLKYHEILAKKRVNESPTVADAISHATCQTALDLSAKAIISSTHSGSTARMVSKYRP
                     RVPILAATPSERVANKLTISWGVYPIVVSLYYDIDSMLDVSVTAAKKSGLIQKDDLIV
                     ITAGVKTGTPGSTNLLKVHNVE"
     gene            2716..>3006
                     /gene="trxA"
                     /locus_tag="VF531_01835"
     CDS             2716..>3006
                     /gene="trxA"
                     /locus_tag="VF531_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7712737.1"
                     /translation="METKLWEGKVSMKEFDQSNFQEQVLHSNQPVLVDFWAPWCGPCR
                     MVGPIVEEIATDLAGKLQAGKINVDENPALASEYNVMSIPTICLFVKGSPVMK"
BASE COUNT          764 a          647 c          826 g          769 t
ORIGIN      
        1 aacgatttcg ctgtttcggt gagtatgagg aatcctgacg gagagacggg atttaaaaca
       61 agtatctttc atatcttata tcaaaataag agttttcatt atcataaata atttatcagc
      121 gaaaatatgg aggcggaatg atgagaatcg gtgttttgac gagcggcggc gatgctcccg
      181 ggatgaatgc cgcaattcgt tccattgtac gaactggctt gtatcacggc tgtgagatgt
      241 acgggattaa gaaaggtttc agcggtatct taagtgggga aattatcccc atggattcgt
      301 tatccgtagc cgatattttg caacgcggcg gtacgatctt gcagtctgcg cgttgcctgg
      361 aatttaaaac tcccgagggt caggccaagg cgaaacagca attggagaat ctgggattaa
      421 acggcctggt catcatcggt ggcgatggtt ccttccatgg cgcacaagtt ctcaataaaa
      481 tgggttttca aaccgtgggg attcccggta cgatcgataa tgacatcgga tgtaccgatt
      541 attccatcgg ttttgacaca gcagtaaata ccgtgctcga cgccatgaac aagattcgtg
      601 atacggcttc ctctcataat cgggtttgtg tgatcgaggt tatgggaaaa aatgccggat
      661 ttattgcggt ggcagcaggc ttggccggcg gtgccgaagc ggttttaatc ccggaggtgc
      721 cttacaacct ggaggagtta tcgtcccaga tcattcagaa taacgttcgg ggcaaggttc
      781 atagcattat cctggtggct gagggtcttt tcggggatcc aaccagtctt gatcactcca
      841 gcgcctttaa ggtgggaaat tacctttcat tgcggaccgg agccgatact cgtgtcacga
      901 ttttgggtca tttgcaacgg ggcggtacgc catcgtattt cgaccgtaaa cttggcacct
      961 tgatgggcgc cgaggcggtg gaactgatac ttgccggtga gaccgaaaag atggttggct
     1021 tcacccaaaa ccggattcag gtatatgatc tggacactgc gatcaaaacc aaaaaaactg
     1081 ttgatcccga tgagctcgaa ttggtcaatg taatggccaa catatgattt tttgtatcaa
     1141 attggtataa tatcggttat cctatatata atgcagatag aaccatggaa tcgacgtgtg
     1201 cggcaagaga attatcgtat gattgattgc acgtgaacgt tatatttgct gcctcgtttt
     1261 ctcgggagtg ggatcatgaa aaaaacaaag gtcatctgta cattgggacc gtccagttcg
     1321 gaccatgcgg tgctggaaaa aatgatcaat gccgggatga acatcgcccg cctaaatttt
     1381 tctcatggga cttacgagga acatggggcc cggattaagt tggtccggga tgtagccagt
     1441 tcgttaggta aaccggtggg gattttggcg gatatccgcg gaccgaagat ccgcaccggg
     1501 attctcagtg aagaaccttt gattctttcc gatggttctc gaatccggct gagtatcgac
     1561 cagggaaagg aacaaacccc gggttatatt tacgttgatt atccttcact tctccaggac
     1621 ctgcacccgg gaagcaaaat ctttctggcg gatgggatga tcgagcttca ggcggttcag
     1681 atcggatcca acgatgtgga gtgcgaggta acgagcggcg gagaattgac ttcgcgcaaa
     1741 ggtgttaccc ttcccggagt atctgtaaat ttgccagcct tgatggataa ggaccgacgt
     1801 gatatcgcct ttctcgtcga gcaaaagatt gactttatcg ccgtttcctt cgccaggaaa
     1861 gccgaacata tcgtagagat tcgtaaactt ctccaagact tgggcggaga tcaactatta
     1921 atcgccaaga tcgagaatga agaagggtta cacaatgtcg atgagttgat caaagtttgc
     1981 gatggtatta tggtagcccg gggtgatctg ggagtggaag ttcccctgga ggaagttccc
     2041 ctgattcaga aatatctgat tcaaagatgc aatttggccg ggaaaccggt aattacagcc
     2101 actgagatgc tggagtcgat ggttcgcaat ccccggccga cccgggcgga gatcaccgat
     2161 attgcacatg ccattaccga tggtaccgat gctattatgc tctcggcgga gactgcggtg
     2221 ggcaaatatc ctgtggctgc agtcgagatc atgacccgag tggcggaaaa gatcgagggt
     2281 tcgctgaagt atcacgagat ccttgccaaa aaacgggtga atgagtcacc cactgtagcc
     2341 gatgccatca gtcatgccac atgccagact gccttggatc tatcggccaa agcgattatc
     2401 agttccactc attccgggag cacggcacgg atggtgtcca aataccgccc ccgtgtccca
     2461 attttggctg ctactccttc ggagcgggtc gctaataagt taacgatttc gtggggggtt
     2521 tatcctattg tagtctccct gtattacgat atcgacagca tgttggatgt cagcgtcact
     2581 gcggccaaga aatccgggtt gattcaaaag gatgacctga ttgtcatcac tgccggtgtg
     2641 aagaccggga ctccgggttc gaccaactta ttaaaagttc ataatgtgga atgatatatt
     2701 tgctcggacc ggtggatgga aacgaagtta tgggaaggga aggtttcgat gaaggagttc
     2761 gatcaatcta actttcagga acaagtcttg cactcaaacc agccggtttt ggttgatttt
     2821 tgggctccct ggtgcggacc gtgtcgaatg gtaggcccga tcgtagagga gatagcgacc
     2881 gaccttgccg ggaagttaca ggcgggtaag atcaatgtgg atgagaatcc cgcgctcgcc
     2941 agcgagtata atgtgatgag catccccacg atctgtttgt ttgtaaaggg tagcccagta
     3001 atgaaa
//
LOCUS       DASVMB010000054         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_5109,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000054 DASVMB010000000
VERSION     DASVMB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5109"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF531_01840"
     CDS             complement(<1..413)
                     /locus_tag="VF531_01840"
                     /inference="COORDINATES: protein motif:HMM:NF028185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7712738.1"
                     /translation="MSILYDESQQIFHLQSKEMSYVIHVLKPGYITHLYWGKRLRTAN
                     LLYLLQLRERAFSPTLDPANPALSLDTIPQEYPAYGNTDFRAPAFQIQFENGSTVSDL
                     RYHGYTIHQGKPELEGLPATYVEDVSEATTLDLELR"
     gene            622..1221
                     /locus_tag="VF531_01845"
     CDS             622..1221
                     /locus_tag="VF531_01845"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEX7712739.1"
                     /translation="MAPSHQNTRALLVQAGTTYPKVINLPSHSAEHKVNTIIRATAYQ
                     AIPTYNPGTSIIEAQSGYSTMLNQNGILSLRFQDYIYPEMAAHGVTQYSSVTLNLETG
                     YNYRFDELFRPGADYQARINQIIQAQISARQIPMLKPFAGVGSDETYYLTPDRLVIYY
                     QPYVYTPGYYGILEFAIAYSQISDIINPAGPIGRILPRV"
     gene            complement(1313..3151)
                     /locus_tag="VF531_01850"
     CDS             complement(1313..3151)
                     /locus_tag="VF531_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7712740.1"
                     /translation="MKNPLNKVTGLFQSSVFSRVKVFYQILIVILVMLFFIGLEGIGS
                     LRTVDTMHQNTQQLFNQSVKEINDVHTVLVNMEIAQRNYLEQLKYGGANYAGSQVDQL
                     LTDARNLTVLDEIAKQNVLRELEQAQQLTQAAPSDANYQALSRSFLIMKLELEKVYNA
                     ALTNGINTIQGSKEYSRRTRMSLIVTLILSSLIAVLLGLLIASSISRPLRGMMNAAES
                     LSHGDLTKQIKMTGCLEVNQVVRELNSAITGLRELTLKVNNQAEELAVASKELKGSSF
                     ETGRSAGEVARAMEELAKGSTEQADQIGKAVNTVNLLAEMVRKVSMDTESIANASQHV
                     ADSAQMGKKATGDVIHQMNELYDSTKEAAGVIDELSRVSGAIGEITSMIENIAGQTSL
                     LALNASIEAARAGEYGKGFGVVARKTAKLAEESKQAAKDISDLITQMHVHTDHAVEAM
                     QKGISRAQQGKEYVDKAGETFETIFDALMDNLNHINEVADSARRMAESNEAVVGTISA
                     IAAISEESMASTEEVSATAQQQTASVEEVTSLADHLSRIADNLKQSVSAFKLDSSDQQ
                     LPTESNDENGSITGRIEGEMLSDRRPLFLDENPEPARNDGIDEFHD"
     gene            complement(3207..>4068)
                     /locus_tag="VF531_01855"
     CDS             complement(3207..>4068)
                     /locus_tag="VF531_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7712741.1"
                     /translation="ANEVAKAMDDLAKGSMEQTEQINQAVLTVNRLAELVRKVTEDTG
                     RITKASEQVAESAQLGKKASNDVTRGINELFDFTREVSGMINRLNDASGQISEITAMI
                     ENISEQTSLLALNASIEAARAGDYGKGFGVVARKTAKLAEESKQAAQDITGLIVQMRN
                     QTEHAVEIMQKGINQAQSGKEAVDKAEQTFGSIFDSLSENLNRIREVATSAHLMGESN
                     ESVVNAITTIAAISEESSASTEEVSASTQQQTASIEEVIALADNLSQIAEDLKRSVAA
                     FKLEEESQIG"
BASE COUNT          993 a         1087 c          895 g         1093 t
ORIGIN      
        1 cggagttcca gatccagagt ggttgcctct gaaacatcct ccacatatgt ggcgggaagc
       61 ccctctaatt ccggtttacc ctgatggatc gtataaccgt ggtaccgcaa atcgctcact
      121 gtagagccgt tttcaaactg aatctgaaat gccggcgccc ggaaatcagt gttgccgtat
      181 gcgggatatt cttgcggtat tgtatccaaa gataaggcgg gattcgccgg gtccaaggtc
      241 ggtgagaaag cacgttccct aagctgcaat agatataata aatttgccgt acgaagccgt
      301 tttccccagt aaagatgcgt aatataacct ggtttcagaa catggatgac ataactcatt
      361 tccttagatt ggagatgaaa gatttgttgg gattcatcat aaaggatact catgggacct
      421 ccgcacttca ctaaggtgta taaaaaccaa atatgttact atccggcata aaaattgtga
      481 tttgaaaaaa gcggcatgaa caaacatcga tactcatcac accgcttttc tcgaaatgct
      541 tctaaacgag aaacgccatc acacactgtg ccttaggacc atagattata tgaatcaaaa
      601 aaagtaaagg gggtactaaa aatggctccg tctcaccaaa acacaagggc attactggta
      661 caggcgggaa cgacgtatcc aaaagtaatt aaccttccca gccactctgc ggagcataag
      721 gtaaatacga tcatccgcgc aaccgcttat caagccattc ccacttataa tccgggcaca
      781 agcattatcg aggcgcagag tggttacagc acaatgctga atcaaaacgg aattcttagc
      841 ctgcgtttcc aagattacat ttatccggag atggccgccc atggggtgac gcagtacagt
      901 tcggtcacgc tcaatctcga aaccggatac aactaccgtt tcgatgagtt gtttcgaccc
      961 ggagccgatt atcaagcccg gatcaaccaa atcatccagg ctcaaatcag cgcccggcaa
     1021 attcccatgc taaaaccgtt tgccggagtc ggctccgatg aaacctatta cctcaccccc
     1081 gatagattgg tcatctatta tcagccctac gtctatactc cggggtacta tggaatcctg
     1141 gaatttgcca ttgcctactc ccagatctcc gacattatca atccagcggg gcctatcgga
     1201 cgaattctgc cgcgggttta aagtttttaa cctctcgatt caaaaaaacc ggcctaagaa
     1261 gagaggccgg atacttcgca tcattttgtt agtttttctt gataacactg atttagtcgt
     1321 ggaattcatc aatcccatca ttacgagcgg gttcgggatt ttcgtccaaa aacagcggcc
     1381 ggcgatccga aagcatctcg ccctctatcc gcccggtaat actgccattt tcatcattcg
     1441 attcggtcgg caattgttgg tcggagctat ccaacttaaa cgcggaaacg gattgtttca
     1501 agttgtcggc aatcctcgat aaatgatcgg ccaaagaggt cacttcttca acggatgcgg
     1561 tttgctgttg ggcagttgcc gaaacttcct cggtgctcgc catgctctct tcgctgatgg
     1621 ccgcaatggc tgagatggtt ccaaccactg cctcgttgct ttccgccatc cgacgtgccg
     1681 aatcagctac ttcattgata tgattaagat tatccatcaa agcgtcaaaa atcgtctcaa
     1741 aggtttcgcc agccttgtcg acgtattctt taccctgttg tgcccgggaa attccctttt
     1801 gcatagcctc cactgcatga tcggtatgta catgcatctg cgtgatgaga tcggagatat
     1861 ccttggcggc ttgtttggat tcctccgcaa gtttagctgt tttccgcgcg accactccaa
     1921 accctttgcc gtactctccg gcccgggccg cttcgatcga agcatttaag gccaacaatg
     1981 aagtctggcc tgcgatgttc tcaatcatcg aggttatttc gccaatcgcc ccggagaccc
     2041 ggctcagctc atcgatgacc cctgccgctt ccttggtgga atcgtagagt tcgttcatct
     2101 ggtgaatcac gtcacctgta gcttttttgc ccatttgagc cgaatccgcc acatgttggg
     2161 aagcgtttgc aatactctcc gtgtccatac tgaccttacg gaccatttca gccagaagat
     2221 taactgtatt cacggccttg ccgatctgat cggcttgttc cgtcgaacct ttggccagtt
     2281 cttccatggc cctcgctact tcacccgcgg atctaccggt ctcaaaagag ctccccttca
     2341 attctttact ggcgaccgcc agctcttccg cctggttgtt aacctttaaa gttaactccc
     2401 gcagaccggt gattgccgaa ttcaactccc gaacaacctg gttcacttca aggcaaccgg
     2461 tcatcttgat ctgctttgtc agatcaccgt gggacaacga ctcggcggcg ttcatcatcc
     2521 cccggagcgg gcgagaaatg gaactggcaa tgagcaagcc aagcaaaacc gcaatgagtg
     2581 agcttaagat taaggtaact atcagactca tcctggttct ccgggaatac tccttactcc
     2641 cttggatcgt attgatcccg ttcgtcaacg ccgcgttata cactttttcc aattccaatt
     2701 tcatgattaa gaaagaacga cttagggctt gataattggc gtcgctgggg gcagcctggg
     2761 ttaactgctg tgcttgctca agttccctga ggacattttg tttcgcgatt tcatccagca
     2821 cggtcaagtt ccgtgcatcc gtcagtaact gatcgacctg ggaaccggca taattggcgc
     2881 cgccatactt taactgctcc aaataattgc gctgggcgat ctccatattc accaaaaccg
     2941 tgtgaacgtc gttaatctcc ttgacactct ggttaaacag ctgttgcgtg ttttgatgca
     3001 tcgtgtcgac agttcgtaga ctgccgatac cctcaagtcc aataaaaaag agcataacaa
     3061 gaataacaat gagaatttgg taaaatacct tcacccttga gaataccgaa gattgaaaca
     3121 gtcccgtcac tttattcaat ggatttttca tcgctaacac ctcgatgatc ataatacttt
     3181 tatcttcggc agatttcgtc aagaacttaa cctatttgac tttcctcttc aagcttgaac
     3241 gcagcgaccg atcgcttgag atcttcggcg atctgcgata agttgtctgc cagcgcgatt
     3301 acctcctcga tcgaagcggt ttgttgctga gtggaggccg aaacctcttc ggtgctggcg
     3361 gaactctctt cactaatggc tgcgatggtg gtgatcgcat tgaccacgct ctcgttactc
     3421 tcacccatga ggtgggcgga tgtcgccact tcccgaatgc gattcaggtt ttccgataat
     3481 gaatcaaaaa tagatccaaa ggtttgttcg gccttatcca cggcctcctt acctgattga
     3541 gcctgattaa tacccttttg cataatctcc accgcatgtt cggtctgatt acgcatctga
     3601 acgatgaggc cggtaatatc ctgggcggcc tgtttggatt cttcagccaa tttggcggtt
     3661 tttcgggcca ccactccaaa acctttgccg taatctccgg cccgcgccgc ttcgatggag
     3721 gcattcaaag caagtaacga tgtctgttcg gagatatttt cgatcattgc ggtgatctca
     3781 ctaatctgtc ctgaagcatc attcaaccgg ttgatcattc cggaaacttc ccgggtgaaa
     3841 tcgaacaatt cattaatccc ccgggttaca tcgtttgaag ccttcttacc caactgggcg
     3901 gactctgcaa cctgctcgga tgccttggtg atccgccccg tatcctcggt gactttccgg
     3961 accaattccg ccaaccggtt cacggtaaga accgcctgat taatctgttc cgtctgttcc
     4021 atggaaccct tggccaaatc atccatagcc tttgcaactt catttgcg
//
LOCUS       DASVMB010000055        10450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_152463,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000055 DASVMB010000000
VERSION     DASVMB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152463"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF531_01860"
     CDS             <1..548
                     /locus_tag="VF531_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7712742.1"
                     /translation="EKLLDELLEFSRMESDGFQLQLAETNLAELAREILIEYLPFIKE
                     QDINLQADIPDSPVSARVDPNRISRVLRNLIDNALKYGSQGKYLRVAVTSTQTTISIE
                     VEDRGPGITETEQAKIFDRFYRIDKGRNSAAGGAGLGLAIAREIVRKHGGQIRVTSEK
                     GKGTIFGVELPATSTQESKPD"
     gene            complement(591..995)
                     /locus_tag="VF531_01865"
     CDS             complement(591..995)
                     /locus_tag="VF531_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX7712743.1"
                     /translation="MAIEWTEDLAVGVLEIDNQHKELFRKIDQLLEACNQCRGKEVVG
                     ETIRFLGDYVIEHFGNEERYMKQFNYPESAGHMEQHRQFIDSFQELKRKFESDGPGTH
                     IVIMTNRAVIGWLNHHIRNVDKLLGEFLKTKL"
     gene            complement(1050..1604)
                     /locus_tag="VF531_01870"
     CDS             complement(1050..1604)
                     /locus_tag="VF531_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX7712744.1"
                     /translation="MAVQNAMTADFLRSAYGGESMAHMRYIAWGNLAEKEGFPNIGRL
                     FKAVSYAEQVHANNHFRELGIRVGDHTVTAGAVFGIGSLVDNLQGAINGELHEVEQMY
                     PVYLQTAQFQDEKGAQRSFHFALEAEKIHARLFKEAQEKAKEGKDLQLNSVNICPICG
                     HTVVDGIPDNCPVCGAKKEMYVTF"
     gene            complement(1753..2175)
                     /locus_tag="VF531_01875"
     CDS             complement(1753..2175)
                     /locus_tag="VF531_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX7712745.1"
                     /translation="MKILYKTKAVSSGGRDGKVQIENSPLEFEMAVPAELGGSGKSGV
                     NPEQLFAAGYAACFESSLLHVARRRNLTLNSTSVAVEVGFGPNDKGGFRITAFITATV
                     SGVDQVTADALVQEADQVCAYSNAIRGNIETTVSARIA"
     gene            complement(2194..2808)
                     /locus_tag="VF531_01880"
     CDS             complement(2194..2808)
                     /locus_tag="VF531_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7712746.1"
                     /translation="MDLKKVFEERRSVNFFKPDCPIEKDVLRNIIKLAALTPSAFNLQ
                     PWEIIAVQSAAAKEKLFSLAGNQLKIKEAPVTLIIIGDLEGYQPENPAWDDLEAMLGS
                     KQALEEYQKAAHALYGSSEDGRIKFAESNAALLAMSIMYAAKYYGLDTHPMSGIDFEG
                     IRKEFGLDENKTVVMLIALGYFDDSKTLYPRRMRKDYEDIVKEV"
     gene            complement(2883..3365)
                     /locus_tag="VF531_01885"
     CDS             complement(2883..3365)
                     /locus_tag="VF531_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7712747.1"
                     /translation="MDALEVAMKMEQDGENFYRELSQKAINPGFREIFNQLADDEVDH
                     YQLFLTIQHSPESLMEASDVKNAANLFKQMIARGDLSDLDSTQLELYQKAMDLEKESR
                     DFYLKKAMEAAAQNQRELFQKIAAEEERHYFLLHDMYELVLHPQTWVENGEFVHLEEY
                     "
     gene            3997..4317
                     /locus_tag="VF531_01890"
     CDS             3997..4317
                     /locus_tag="VF531_01890"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7712748.1"
                     /translation="MAEIHTGEEVKLLAKKYGEVLRQKYDLHSLYLYGSYVSGNYTSD
                     SDIDIAVIAADFSGDIFEDTLNLMRYRRLVDNRIEPHPFIPSEFNFTNPNAREIMAKG
                     IRII"
     gene            complement(4768..7089)
                     /locus_tag="VF531_01895"
     CDS             complement(4768..7089)
                     /locus_tag="VF531_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX7712749.1"
                     /translation="MVKQVKILEWIVQDDRFRMDHVEQNGNKYLIANGYMGYRGTLEE
                     YSKDQLVACILSGIYDRVGNAWREPVNAPNGFLVRLICDGEPLSVLDSDVKSHSQSLD
                     IQNGIHHRKTTFRTKSGKRMTVASDRFTSLANVHMMAMRYSIQWDHDSEIEVITGIDG
                     DIWDINGPHLENPSLTFREQYLLYTALTHELGHKVVTAETIICPFGNQDIDIAAGSIF
                     HRIRFRAQAENEYRFIKYVTVFTSVDASDPEKEATDLIRKISCGEYDHHLADHMKLWH
                     GRWDRSDVQINGDPDAQQALRYSIYQLLSIAPTHTDKTSIPARGLSGQTYKGAVFWDT
                     EMFMLPFFIYTQPEIARKLMKYRYHALDGARRKAAEYGFRGAFYAWESQETGADACTH
                     FNVTDVFTGRPLRTYFRDKQIHISADVAYGIWQYYALTGDAGILLDGGAEVILECARF
                     FYSYSYYQMEKQRYEILDVTGPDEYHERVNNNAFTNQMVKHTLDIALKVGELLQGSHP
                     DFYRYLLDKLDYHQDFHKIKEMSAQLYIPQPDQNSGVIEQFDGYHHLEDISLPELRKR
                     VLNPNEYWGGGNGIASTTKILKQADTILMLHIFKNDYSDEIKKANWCYYEPRTEHGSS
                     LSACVYALVAADCGDAEWGYQYFMKTATIDLTGESKQYVGTLYIGGTHPAANGGAWMA
                     AVLGFAGLNSDGETLRFNPCLPAQWESIAFKFEWQGCKFGVVIDQEELQLESDQANPK
                     EMTICINGEMLLCKAGQQIKVLLTRGGLSYDEA"
     gene            complement(7149..8051)
                     /locus_tag="VF531_01900"
     CDS             complement(7149..8051)
                     /locus_tag="VF531_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712750.1"
                     /translation="MSRFATKNTGLLNFMDLKNKPGKIFYWSIFGFMCLLAIICLFPP
                     VWLFFSSIKNVQELYSIHPTFIPKTFEWKKIFFVWNQLDFLRYYVNSVEMALGDIVFS
                     LVFNSLFGFVLSQLKPKGSSFLMALLLWTLLLPNTINIVPVYLNIINFPVLHINFSNT
                     FLPMWFMSGANAFFTLIFKSFFDGIPRSLSEAAMLDGCSPFKIFLKIFLPLSKPVMFV
                     IVIFTLTGSWSNFFWPYLVLTNKDLYTVMIRIFTMTNGNLSGFSVDYQMMAIVYTMIP
                     PIIIFFLLQKYIMQGGLTLGSIKG"
     gene            complement(8055..8948)
                     /locus_tag="VF531_01905"
     CDS             complement(8055..8948)
                     /locus_tag="VF531_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712751.1"
                     /translation="MGSAFRKTVRKDIFAWALMLPSLLMFTFFTWQPLISGIVLSFFQ
                     TKGYRAVKFIGLGNYQEIITNSSFKAAFTNSASYTFWSLLIGFTIPIIVAILLNEIIH
                     GNAFFKFAFYFPGMIPGVATYLMWGFMYNPSKWGVFNSILTGLGWYQFGWLNVPQFTI
                     PLIVVTMTWAAFGGTALIYMASLQGINQELYEAASLDGAGFFRKVRYITLPAMSNIIL
                     LMLIMQIISVFQVMIQPLSMTGGGPSDASLSLMLQSYLYAFNYFKVGPSMAAGVITFL
                     ILLIPTAVYFIITKKDRESEV"
     gene            complement(9047..>10450)
                     /locus_tag="VF531_01910"
     CDS             complement(9047..>10450)
                     /locus_tag="VF531_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712752.1"
                     /translation="PAIHNKEECGMLKRQVVIGLSFALLVSVFSVVGLAQPKVTISVG
                     AWPRENEPERLATAKKFAKIMLEKYNVEVVGDEWQYAVDSFIPKATGGTLPTVYNTWF
                     TEPQKIIDNGYAANITKQMKKYGLDKAINPQILSILSKNSKIYGIPDNGYVQGLACNV
                     KLFKQAGLVDAGGVPKAPKTYEELAQTAKIIKDKTGAAGFIMETSNNCGGWHFMNIAW
                     SFGTVFEKKIKGKWTATFNNPEGVAALQYLKDLKWKYKVLPDTALVDWAGGQQIFATN
                     QGAMFFFGGSYHGGFQFAIDNYKIGKDDISMFSLPAGPKGRYALVGGNVWMFAPNATP
                     AQIEAGIHWLDLNGLSPKVSEEFKLSEEANYKTDKDAGYVVGVDGLPVWVNPEKTKIT
                     EAIRQKYLNVNPGLFADYNNMKQVTLKPEEPVACQELYKALDKAVQTVLTDSNADVKA
                     VLDKTAADFQKDVLDRQ"
BASE COUNT         2916 a         2504 c         2151 g         2879 t
ORIGIN      
        1 tggaaaagct gcttgatgaa ttactggagt tttcccggat ggaatcggat ggcttccaat
       61 tacaactcgc cgagaccaat ctggctgaac tggcccgcga gatcttaatt gaatacctgc
      121 cattcatcaa agaacaggac atcaatttac aggcggacat tccggattcg ccggtttctg
      181 ccagggtcga tcccaaccga atctcccggg tgcttcgtaa cctgattgat aacgcactca
      241 aatatggttc tcagggcaaa taccttcggg tagcggtaac ctcgacccaa acaacaatca
      301 gcattgaggt tgaggaccgt ggccccggca tcaccgaaac ggaacaagca aaaatcttcg
      361 accgctttta ccggattgac aaaggacgta actccgcggc aggcggggcc ggtttggggc
      421 tcgccatcgc ccgggaaatt gtgagaaagc acggcggtca gatccgggtt accagtgaaa
      481 agggcaaagg aaccatcttt ggcgtagagc ttccggcaac ttctacgcag gagtccaagc
      541 ctgattaggt acgaaatgaa gccggtaaaa aaagaaaggc gtttacgcct tcacaatttt
      601 gtcttcaaaa actctcccaa taatttgtca acattgcgga tatgatggtt cagccatcct
      661 ataaccgccc ggttcgtcat aatgacaata tgagtcccgg gaccgtcgct ttcaaacttc
      721 cttttgagct cctggaaact gtcgatgaat tgccggtgtt gctccatatg gccggcagac
      781 tccgggtaat tgaattgttt catatacctt tcttcgttgc caaaatgttc gatgacataa
      841 tctcccaaaa atcgaatggt ttcaccgact acttccttcc ctctgcattg gttgcaagct
      901 tccagcaatt gatcgatttt tcgaaacaac tctttatgct ggttatcaat ctccaataca
      961 cccaccgcca aatcttccgt ccactcaatg gccatccctg acacctccga cttcggttta
     1021 caagctccca ggcattttaa aaagggaatc taaaaagtca catacatctc tttcttggcc
     1081 ccgcagaccg gacaattatc cggaatgccg tcaacaacgg tatggccgca tatcgggcag
     1141 atgtttaccg agtttagttg caggtccttg ccctccttgg ctttttcctg ggcttcttta
     1201 aacaaccggg catgaatttt ttcggcctcc agcgcaaaat ggaaggaccg ctgcgcacct
     1261 ttttcatcct gaaattgcgc tgtttgcaga taaaccgggt acatctgttc cacttcatga
     1321 agctcgccgt tgattgcgcc ctgaagatta tcgaccagac tgccgatgcc aaaaaccgct
     1381 ccggcagtta cggtatgatc gcccacccgg atgcctaatt cccggaaatg attgttggcg
     1441 tggacctgtt cggcgtagga aacggcctta aacaacctgc cgatattcgg aaaaccttct
     1501 ttctccgcca agttgcccca ggcgatatag cgcatatggg ccatgctttc accgccgtaa
     1561 gccgaacgta agaaatccgc agtcattgca ttttgaaccg ccatttttta cccctccaat
     1621 attgttttta ttccaagtaa catatgataa gtatcaacca gaagtcgaat tcgtgaccgc
     1681 ccgttatttt tattgtatcc tctgaaagct catttattca gctttcagag gatttacaac
     1741 ctgccacttt gattacgcga tccgggcgga gacagtcgtt tcaatattgc ctcggattgc
     1801 attggagtat gcacagactt gatccgcttc ttgaaccagg gcatcggctg taacctgatc
     1861 aacccccgaa accgttgccg tgatgaacgc ggttatccga aaaccgcctt tatcgttcgg
     1921 cccgaatccg acctctaccg caacggaagt tgagtttaaa gtcaaattcc ggcggcgggc
     1981 cacatgtaaa agagaacttt caaaacaggc cgcatagcct gcggcgaata attgctcggg
     2041 gttcacgccg cttttgccgg aacctccgag ttccgccgga actgccattt caaattccag
     2101 aggactgttt tcaatttgga ccttgccgtc acgcccgccg gaagaaaccg ctttcgtttt
     2161 gtaaagaatt ttcatctcat ctacctcctc gtattaaact tcttttacaa tatcctcgta
     2221 atctttccgc attcgccttg gatacaatgt cttcgagtca tcgaaatatc ctaaggcaat
     2281 caacattaca accgttttgt tttcatccag gccaaactcc ttccgtatcc cctcgaaatc
     2341 aattccgctc atgggatgag tatctaaacc gtaatacttc gccgcgtaca taatcgacat
     2401 ggcgagtaac gccgcattac tttcggcaaa tttgattctt ccgtcttccg atgaaccata
     2461 taaggcatgg gcagctttct gatactcctc gagcgcctgt ttactcccaa gcatggcctc
     2521 cagatcatcc cacgccggat tttcgggttg atatccctct aaatccccga taataatcaa
     2581 tgttaccggt gcctctttga ttttcaattg attaccggcg agcgaaaaaa gtttttcttt
     2641 cgccgccgcg gattgaaccg caataatctc ccacggctgt aaattaaacg ccgatggcgt
     2701 aagtgcggca agcttaatga tatttcgcaa gacatccttt tcaatggggc aatccggctt
     2761 gaagaaattc accgaacgcc tttcctcaaa aactttcttc agatccatat gaaccaccac
     2821 tcctcttctt tgggggattc gttataaatt acctggctgt tttaagccgg ttcaccggcc
     2881 ggtcaatact cctccaagtg gacgaattcg ccgttctcaa cccaagtttg agggtgcaga
     2941 accagttcgt acatatcatg gagtaagaaa taatgccttt cttcttcggc ggcaatcttt
     3001 tgaaacaatt cacgctgatt ttgtgcagca gcttccatag cttttttcag gtaaaaatct
     3061 cggctttcct tctcaagatc cattgctttt tggtacagtt ccaactgggt agagtccaga
     3121 tctgacaaat cacctcgagc gatcatttgt ttaaataggt ttgcggcatt ttttacgtcc
     3181 gatgcttcca ttaatgattc cggcgaatgt tgtatcgtca aaaataattg ataatgatcg
     3241 acctcatcat ccgcaagctg gttaaatatc tcccggaaac ccgggttgat ggctttttgg
     3301 gataactccc ggtaaaagtt ttctccgtct tgttccattt tcattgcgac ttccaaggca
     3361 tccatagcat gattcctcct ctttttcgtt acttttttcc tgcaaccttg attgccatga
     3421 tgtatatcct ttccgttatt aattattaac tatgtttaat ttcaatatga ttatacatta
     3481 ccccgggtta tttttcaacc cctttttgaa gaaaaatact ctactcaacc ggtaccccta
     3541 taaagctcag cgaagattat cccctaattt gttgcgtgaa acaggtaata tcaatacccg
     3601 cccggtcggt gaagacggct tggattttgg cggttttttg tgcatcgggg aggtctcctt
     3661 tataaagttt aaagttgtga atttcggttt aagggaacaa agaatcaaat cattcccgcg
     3721 ctttaagggg aaaaagggtt atggtataat taagtaaata gacagaaagc ttaatttcag
     3781 aattctattt ctccgaaata gtaacaataa attatgatga ttctaaggta ggtgccgccg
     3841 acaatgacga aggaagaatt aatcgcctat tggcgcgact cagccgaacg cgactttgaa
     3901 acaatggagc attcttgatt ataaacaaga cttttatcat aaatgtacgg aagaatttac
     3961 ttcccagatg atcgacaaaa tcaaggagat gagcttatgg ctgaaatcca tactggagaa
     4021 gaagtaaaac tacttgctaa aaaatatggg gaagttttgc gacaaaagta tgatttgcat
     4081 agtctctatt tatatggctc ctatgtgagc ggaaattata cttccgacag tgatattgat
     4141 attgcggtga ttgcggcaga tttttccggc gatatcttcg aagatactct gaatttgatg
     4201 cgttatcgca gattagtgga caaccggatt gaaccacatc cgtttattcc gagcgaattt
     4261 aacttcacta acccaaatgc ccgggaaata atggctaaag ggattcgtat catttaaccc
     4321 gctcacacca tgatcctctc tactccccta cccaccatag ccacctaata gctgaataca
     4381 atcccgtcca tcaacccttc gctatagatg atctcatcct ggcggttgat ttgatagatc
     4441 agcagagcca ctttaaatcg gtgggttaag gccgggaaga ttcaggcttt aaaactaggt
     4501 gaaggaagaa aaggtacggt aaggtttaag ttgagagata ttgaggagtt tattgataac
     4561 ataaagaaac aatcgtaaaa aaatcatctt aaaaagcaga gttatgaaga agtgaaaaat
     4621 aaaccagccc cttagctggt ttcatttctt tttggtaatc ataaaccgta cttaaccggc
     4681 gttgccacct tgtacataat agagctgcat attccggatg aaactggaca ccgattccgt
     4741 ttgaaagtgg acagaccttc tggtactcta ggcttcatcg taggaaagtc caccacgagt
     4801 caaaagtact tttatttgct gccccgcctt acaaaggagc atctctccgt taatacaaat
     4861 tgtcatttct ttggggttgg cttgatccga ttcaagttgc agctcttctt ggtcaataac
     4921 gacgccgaac ttacagcctt gccactcaaa cttgaaagcg atagattccc attgcgccgg
     4981 taaacatggg ttaaaacgca aagtttcacc gtccgaattt aaaccggcaa aacctaaaac
     5041 tgcagccatc catgctcccc cattggcagc cggatgagtt ccgccaatat acagtgttcc
     5101 gacatactgc tttgattcac cggttaaatc gatggtggcg gttttcataa aatattgata
     5161 accccattcc gcatccccgc agtccgcagc gactaaagca tatacgcatg cacttaggct
     5221 ggagccatgt tcggtacgag gttcataata acaccaattt gcctttttaa tctcgtcgga
     5281 gtaatcattt ttaaagatat gaagcattag aatcgtatct gcttgcttca gtattttggt
     5341 ggtagacgca atcccgttac cgccacccca atattcattg ggatttaaaa ctctctttcg
     5401 taattccggt aagctgatat cctccaggtg gtgatagcca tcaaactgtt caatcacccc
     5461 actattttga tcgggttgcg gaatatagag ttgggcgctc atttctttta ttttgtgaaa
     5521 atcctgatga taatccaact tatcaaggag ataccgatag aaatccgggt gactgccttg
     5581 taacaattcg ccgactttga gcgcaatatc tagagtatgc ttgaccattt gattggtaaa
     5641 ggcattattg ttcactcttt catgatactc atccggaccg gtgacatcca ggatttcata
     5701 acgttgtttc tccatttgat aataagaata ggaatagaaa aaccgggcgc attccaaaat
     5761 aacttccgct ccgccatcca gcaaaatgcc ggcgtcaccg gtcaaagcgt agtattgcca
     5821 aatgccgtag gcgacatctg cactaatatg tatttgcttg tcccggaaat aagtccgcag
     5881 cggccgaccg gtaaatacat ccgtaacatt aaaatgagta caggcgtcag caccggtttc
     5941 ctgactttcc caagcgtaaa aggcccctcg aaatccatac tcggctgctt tccggcgtgc
     6001 gccatccaga gcatgatacc ggtatttcat tagttttctg gctatctccg gttgagtata
     6061 aataaaaaag ggcagcataa acatttccgt atcccagaaa acagcccctt tgtaagtctg
     6121 ccccgataac ccccgcgccg gaatagaggt tttatcggta tgggttggag cgatgcttaa
     6181 gagttgatag atactgtagc gcaatgcttg ttgggcatca ggatctccgt tgatttgtac
     6241 atcggaacgg tcccaacgcc cgtgccataa tttcatatgg tccgccaaat gatgatcata
     6301 ttcaccgcat gaaatcttcc tgatcaagtc agttgcttct ttttccggat cgctggcatc
     6361 aaccgaggta aagaccgtta catatttgat gaagcgatat tcattttcag cttgagctct
     6421 aaaacgaatt cggtggaaaa tcgatccggc cgcaatatca atatcctgat tgccgaaagg
     6481 acagattatg gtttcggcag ttacaacttt atgccctaat tcatgggtta atgccgtgta
     6541 taaaaggtat tgttcccgaa atgttaaaga cggattctct aaatgagggc cgttaatgtc
     6601 ccaaatatcg ccgtcgatcc cggtgataac ctcaatctca ctatcatggt cccattgaat
     6661 gctatatctc atggccatca tatggacatt ggccagactg gtaaaacgat ccgaggctac
     6721 ggtcattctc tttcctgact tggttctaaa ggtggttttc ctatgatgaa taccgttttg
     6781 aatatccagc gattggctat ggcttttaac atctgaatct aaaaccgata atggctctcc
     6841 atcgcagatc aagcggacta aaaaaccatt cggagcattg accggttcgc gccaggcatt
     6901 gccgacccgg tcataaatac ccgatagtat acaggctact aactgatcct tggaatactc
     6961 ctccaaggtt ccccggtaac ccatatatcc attggcaata agatatttgt tgccgttttg
     7021 ttcgacatga tccattcgaa atcggtcatc ctgaacaatc cattccaaaa ttttcacctg
     7081 ctttaccatc accataactc atagccattt aacattgatt ggatatcaca aaccctataa
     7141 tatcgggttc accctttgat actgccaagc gtaaggccgc cttgcatgat atacttctgg
     7201 agcaagaaga agataatgat cggcgggatc atggtataaa cgatcgccat catttgataa
     7261 tccactgaaa accctgaaag attgccgttc gtcatcgtaa agatacgaat cattacggta
     7321 tatagatcct tattcgttaa taccaggtac ggccagaaga aattcgacca cgatccggtc
     7381 aaagtaaaga tgacgatcac aaacatcacc ggtttactga gaggcaggaa gatttttagg
     7441 aaaatcttaa aaggcgaaca tccgtctaac atagcagctt cggataagga acggggtatc
     7501 ccgtcaaaaa agcttttaaa aatcaaagtg aaaaaggcat ttgcccccga cataaaccac
     7561 attggcaaaa aggtattgga gaagttaata tgcagtaccg gaaaattgat gatattcaaa
     7621 taaaccggga caatatttat tgtattgggt aacagtagcg tccaaagcaa caaggccatt
     7681 aaaaaggaac ttcctttagg ttttaattga gacaagacaa aaccaaatag tgaattgaat
     7741 accagtgaaa aaacgatatc cccgagagcc atttccaccg agttcacata gtaccgtaaa
     7801 aaatccaact gattccagac gaagaaaatc tttttccatt cgaatgtttt cggtatgaag
     7861 gtcggatgga tcgaatacaa ttcctggacg tttttgatac ttgagaaaaa aagccatacc
     7921 ggaggaaata aacagatgat tgccagtaaa cacatgaacc caaaaatgga ccagtaaaaa
     7981 atcttacccg gcttattttt taaatccatg aaattcagta aacctgtatt cttcgtagca
     8041 aacctagaca ttttttagac ctcgctttcc cgatcttttt tggtgatgat aaaatatact
     8101 gccgtcggta tgagcaagat gaggaaagta atgactccgg ccgccatcga aggcccgact
     8161 ttaaaataat taaaggcata taagtaagat tggagcatta atgaaagcga ggcgtcactt
     8221 ggtcccccgc ctgtcatgga taacggttga atcatcacct ggaacacgga aatgatttgc
     8281 atgataagca tcaaaaggat gatattgctc atggcaggca gggtgatgta ccgaactttt
     8341 ctaaaaaatc ctgctccatc tagcgacgcc gcctcatata attcttgatt gatcccctgc
     8401 aaactggcca tatagatcaa agccgttccg ccaaaggcag cccacgtcat cgtcaccaca
     8461 atcagcggga tcgtaaattg cggcacattc aaccagccaa actggtacca gcccaaaccg
     8521 gtcagtatcg agttaaacac gccccacttc gacggattat acataaatcc ccacatgaga
     8581 taggtggcta cacccggaat catgccggga aagtaaaagg caaatttaaa aaaggcattg
     8641 ccatggatga tttcattgag caaaatcgct acaatgatcg ggatcgtgaa cccgatgagc
     8701 agcgaccaaa atgtatacga agcggaattt gtaaaagccg ccttgaagga gctattggtg
     8761 ataatctcct gataattccc cagcccgata aatttgaccg ccctgtatcc cttggtctgg
     8821 aaaaaagata atacaatacc ggagatcaga ggttgccagg taaagaacgt gaacatcaac
     8881 aaactgggaa gcattaatgc ccaagcgaaa atgtctttgc gtaccgtctt tcgaaatgcg
     8941 gatcccatca aaaaccacct tcttataact tctagattga tacaaatgac ggctttttgc
     9001 cgtcatttgt atcaaattcg cactcaatac cctaaactca acccggttat tgcctatcta
     9061 aaacatcctt ttggaaatca gccgcagttt tatcaaggac cgcttttaca tccgcattgg
     9121 aatcggtaag aaccgtttga accgctttat caagcgcttt atacaactcc tggcaggcaa
     9181 ccggttcttc cggtttcagg gtgacttgct tcatattatt gtaatcggca aaaaggcccg
     9241 gattcacatt aagatacttt tggcggatag cttcggtaat ctttgttttc tcgggattga
     9301 cccacaccgg taaaccatcg actccaacga cataacccgc atctttatcg gttttataat
     9361 tggcttcctc actaagctta aactcctcgc ttacctttgg cgaaagtccg tttaagtcaa
     9421 gccaatgaat acccgcttca atctgcgccg gggtggcatt gggagcaaac atccaaacat
     9481 tgccgcccac taaggcataa cgacctttgg gtccggccgg caagctaaac atggagatat
     9541 catctttacc gatcttataa ttgtcgatgg cgaattgaaa ccctccgtga tagctgccgc
     9601 caaagaagaa cattgcgcct tggttcgtcg caaagatttg ttgcccacct gcccaatcca
     9661 ccagggctgt atccggtaaa accttatatt tccacttcaa gtcttttaag tattggagtg
     9721 ccgctacgcc ttcggggtta ttgaaggtgg cggtccattt tcccttaatc ttcttctcaa
     9781 aaacagtgcc gaaactccag gcgatgttca tgaaatgcca accgccgcaa ttgttgctgg
     9841 tttccatgat aaacccggct gcgccggtct tatccttaat aattttagcg gtttgggcta
     9901 attcttcgta tgttttgggt gctttcggaa caccgcccgc atccacgagc ccggcttgtt
     9961 tgaacagttt aacattacaa gccaatccct ggacgtaacc gttatccggt ataccataga
    10021 tcttgctgtt ctttgacaag atcgaaagga tttgaggatt gatggcttta tcaaggccat
    10081 attttttcat ctgtttcgtg atattggccg cataaccatt gtcgatgatc ttctggggtt
    10141 cggtgaacca ggtattgtaa acggtaggaa gggtgccgcc tgtcgctttg ggaatgaaag
    10201 aatcgacggc gtattgccat tcatcgccaa caacctctac gttatatttt tccaacataa
    10261 tcttggcgaa ttttttagct gtggccaaac gttccggttc attttcccgc ggccaggcgc
    10321 cgacagaaat tgtaactttc ggttgagcca ggccgacaac tgaaaaaacg gaaaccaata
    10381 aagcgaagct taatccaatc acaacttgtc ttttcaacat cccgcattcc tccttgttat
    10441 gaatagccgg
//
LOCUS       DASVMB010000056         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_227089,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000056 DASVMB010000000
VERSION     DASVMB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227089"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF531_01915"
     CDS             complement(<1..190)
                     /locus_tag="VF531_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712753.1"
                     /translation="MKGIGYPLHELDWDTRFFGAKMGEVQLDFAGADFNFSEILWRRT
                     LANARKEHYRFLLCQFCTS"
     gene            complement(241..858)
                     /locus_tag="VF531_01920"
     CDS             complement(241..858)
                     /locus_tag="VF531_01920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7712754.1"
                     /translation="MRRFQLGVKYTLDFVLALVFLIPVGFIILVLGFILYLDSPGPVF
                     FCQLRPGRGGRLFCIYKLRTMVPGDHIATTPRNSDGSLKLTADLKEYTRFGKILRRIS
                     LDELPQIFNILKTEMSFVGPRPDLPEHLALYTETEQIKLAVRPGLTGLAQVRGRNELP
                     WKERLQLDVQYVQKYSWWLDLKILAATVGRIFSGHGVYASESKSR"
     gene            complement(863..2083)
                     /locus_tag="VF531_01925"
     CDS             complement(863..2083)
                     /locus_tag="VF531_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7712755.1"
                     /translation="MDKKRTEKCLILISNFATTPQYPGPTRNFDFACVLAERGWKVKL
                     LNCRFNHYLRRYLHKPPKWQNGVRMAWIWSTPYRGNSIERELNVVVFSALSFWRGLFS
                     RGDIVVTVTPPLETALCGWLLARMKRIPFILDLEDLWPDSIIAMGFRNQAVIKMMRFF
                     EHFLYRHADHIQVVAAEMREYLLKQNVPEAKIDLIPLGANLPRCNRDRATIRAQYGWT
                     DQEIVAVYMGAHGPANSLETLIRAAAKLDDSTIRIVLFGDGSDKPRLREIASELGIGS
                     HLVLADPVPPEVVPDILQAADIGIASLKNTDTFKTVRPNKLYEYMAASLPIVCCIDGE
                     ARRFVTGLDSGIYVPPEDSGELATGLRRLAANPELRRRLGRNGREFVTREGDRRVLAV
                     LMDQVLKKVIDGVK"
     gene            complement(2117..3244)
                     /locus_tag="VF531_01930"
     CDS             complement(2117..3244)
                     /locus_tag="VF531_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7712756.1"
                     /translation="MMLLSNGFAPDPRVAAEASALSEAGHHVTIYAWDRTGDYQAVED
                     WGGVRIVRCPIRTTYSRGPAQILHFFWFWKSCGVFLKKNPFDVVHCHDLDTLWPGIYW
                     GRRLKAPVIYDAHESYPDMVDHLFPKQVVGMVRRLEAKLLPKTTAVITVGELLARRFE
                     GLGAKKVVVVGNYKKLPTTVQGITTVPDFPLRIIYVGGLNRDRLLAPLVESISGERRY
                     QLTVVGDGPQRKSLEGTAAGATNIRFTGFLPQDRARDLIREHHLVYYAIDGSYLNNQF
                     SVPNALFLAMAAGRPVLTTAVGEIAHIVTNYGCGTVLPDLQPDTIRHTLERYLEDNAL
                     WRKQAEQSLHGAITHFNWEYAAKELLELYQDVSSSFHRQIS"
     gene            complement(3285..>3858)
                     /locus_tag="VF531_01935"
     CDS             complement(3285..>3858)
                     /locus_tag="VF531_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7712757.1"
                     /translation="EDLTKSTMPEHPVICSPRFHEPLYNQRVVLEAFRRVITEYPQAE
                     LWFLGDGSQTRELQDLTEKYHLERVRFWGRLSHPEFLKQLASSTVVVSIPSSDATPVT
                     LLEAMAAGKFPVVSDLPVYRDWITDGVTGLICLIDPDSLAATLRRALSDQELWRRAAR
                     LNRDLIVAEGIWEDRFQEMLERYKTLVISD"
BASE COUNT          994 a         1058 c          928 g          878 t
ORIGIN      
        1 ggctggtaca aaactggcaa agtaaaaacc gataatgctc tttcctggca ttagccaggg
       61 tgcgccgcca caatatttcg gaaaaattga agtccgctcc tgcaaaatcc agttgaacct
      121 cgcccatttt tgcaccgaaa aatcgagtat cccaatccaa ttcatgaagc gggtaaccga
      181 tacccttcat cctgaccacc tcattaaccg ccaaacttca aataaaccgt aaagtaccat
      241 ttatcgggat tttgactctg aagcgtagac cccatgcccg gaaaagatcc ggcctacagt
      301 ggctgccaaa atcttaagat caagccacca agaatacttc tggacatact ggacatccaa
      361 ttgcagccgt tccttccaag gtagttcatt cctgcctctg acctgagcga gtccggttaa
      421 acccggacgt accgcaagct taatttgttc ggtttccgta tataaagcga gatgttccgg
      481 aagatccggc cggggcccga caaagctcat ctcagttttt agtatattaa aaatctgcgg
      541 caactcatcc agactaattc gccttaatat cttaccgaac cgggtgtatt ccttgagatc
      601 ggcggtcaat ttcaaggaac cgtcactgtt ccgcggggtg gtggcgatat ggtcccccgg
      661 caccattgtc cggagtttgt agatacagaa caaccgtcca ccccggcccg gacggagttg
      721 gcaaaagaaa acaggccccg gcgaatccaa gtacaagatg aaccccagaa cgaggatgat
      781 gaacccgacc gggatcagga aaactaaggc caacacgaag tcgagggtat atttaactcc
      841 taactgaaat cttctcatat ggctatttta ccccatcaat aacttttttt aacacctgat
      901 ccatcaaaac agccagtacc ctccgatcgc cttcccgggt tacgaattcg cggccgttac
      961 gccccagcct gcgacgaagt tcgggattag ccgcaagtct gcgtaaacct gttgccagtt
     1021 ccccggagtc ttccggtgga acataaattc ccgaatcaag cccggtcacg aacctgcggg
     1081 cctcgccgtc gatacaacaa acgatgggta ggctggcggc catatattcg tataatttgt
     1141 tgggacgaac cgtcttgaag gtatcggtat tttttaagga agcgatcccg atgtccgccg
     1201 cctggagaat atccggaaca acctcagggg gcactggatc cgccaatacc aggtgcgacc
     1261 cgatcccgag ttccgaagcg atttccctga gccttggttt gtcggaacca tcaccgaaga
     1321 gcactatccg gatggtggag tcatcaagct tagccgcggc acggatcaga gtttccaggg
     1381 aattggccgg tccgtgggct cccatatata ctgcgacgat ctcctgatcg gtccagccat
     1441 attgcgcccg gatagtggca cgatcccggt tgcaccgggg caagttggca cccaggggaa
     1501 tcagatcgat cttggcctcc ggaacgttct gcttcaacag gtattctcgc atctcggcag
     1561 ccaccacttg aatatggtcg gcgtgacgat atagaaaatg ctcaaaaaaa cgcatcatct
     1621 tgattacggc ttgattccgg aaacccatag cgatgatcga gtccggccaa agatcctcca
     1681 gatcgagtat aaaagggatc cgtttcattc gagccaaaag ccagccgcac aaggccgttt
     1741 ccaatggagg cgtaacggta accactatat ctccccggga aaaaagtccc ctccaaaagc
     1801 ttagggccga gaataccacg acattgagtt ctcgttcaat ggagttaccc cggtagggag
     1861 tagaccagat ccatgccatc cggactccat tctgccattt ggggggctta tgaaggtaac
     1921 gccggagata atgattaaat ctacagttga gcagtttaac cttccacccc cgctccgcga
     1981 gtacacaggc gaaatcgaag ttccgggttg gtcccggata ctgcggggtt gtagcgaaat
     2041 tactgattaa gatcaaacat ttttcagtgc gctttttatc cattggaatc gtactcacaa
     2101 ggtttcaaat caccggctat gatatttggc gatgaaatga gctgcttacg tcttgatata
     2161 attcgagtaa ttcttttgct gcgtattccc agttaaaatg ggtaattgca ccatgtaaac
     2221 tttgttcagc ctgctttcgc catagtgcat tatcctccag atatcgttcc agcgtatgac
     2281 gaatcgtatc cggttgtaaa tccgggagta cagtgccgca accgtaattt gtcactatat
     2341 gtgcgatctc tcccacggct gtggtcaata ccggccgacc ggcggccata gccaaaaaaa
     2401 gagcattcgg gacactgaat tgattattca ggtaactccc gtcaatggca tagtaaacca
     2461 gatgatgctc cctgatcaag tccctggctc gatcctgagg caaaaaaccg gtgaaacgga
     2521 tattggttgc gcctgcagcc gtaccttcca aggattttcg ctggggcccg tcgcctacca
     2581 cggttaactg atagcggcgc tccccggaaa tggattcgac gagcggtgcc agtaaccggt
     2641 cccggttcaa accgccgacg taaatgatcc gcagcggaaa atcgggaacc gtcgttatcc
     2701 cctggactgt agttggtaat tttttatagt tgccgaccac tactaccttt ttagccccca
     2761 gaccttcaaa ccgccgggct aataactcac cgactgtgat aaccgccgtc gttttgggca
     2821 gcagttttgc ctccaagcgg cgaaccatcc ctacgacctg tttggggaag agatgatcca
     2881 ccatgtcggg ataagactca tgggcatcat agatcaccgg agccttcaag cgccgacccc
     2941 aatagatacc gggccaaagg gtgtcaagat catggcagtg gactacgtca aaaggatttt
     3001 ttttcaaaaa aacgccgcag gatttccaaa accaaaaaaa atgaaggatt tgggctggtc
     3061 cccggctata cgtggttcgg atcgggcaac gcacgatccg gacacccccc caatcttcga
     3121 cagcttggta gtctccggta cgatcccagg cgtagatggt aacatggtgt cccgcttcag
     3181 acaacgcgga tgcttcggcg gccactcgtg gatccggtgc aaaaccgttg gaaagcaaca
     3241 tcacaatctt caagataaac ccccggggaa aaagtcattg gtaactagtc actaataact
     3301 agtgttttat atcgctccaa catttcctgg aaccggtcct cccagatccc ctcggctacg
     3361 atgagatcgc ggttgagccg ggcagcccgg cgccagagtt cctgatcgga aagcgcacgt
     3421 cggagggtcg ctgccaagct atccgggtca atcaaacaga tcagcccggt cacgccgtcc
     3481 gtaatccagt cccggtatac cgggagatca gatacaacag gaaacttccc ggcggccatc
     3541 gcttccaata acgttaccgg tgtggcgtcg ctggaaggaa tactaactac gaccgtgctg
     3601 gacgccaatt gcttcaaaaa ctccgggtgg gacaggcgac cccagaaccg gacccgctcc
     3661 agatgatact tctccgtcaa atcctgtaat tcccgggttt gactaccatc tcccaaaaac
     3721 cacagttcag cttggggata ttcagttatt acacgccgaa aggcctccag caccactcgc
     3781 tgattgtata agggttcatg gaaccgcggg gaacagatca ccggatgctc cggcatcgtg
     3841 ctctttgtta agtcttca
//
LOCUS       DASVMB010000057         5187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_80264,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000057 DASVMB010000000
VERSION     DASVMB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80264"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VF531_01940"
     CDS             complement(<1..708)
                     /locus_tag="VF531_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712758.1"
                     /translation="MELLKVEHLQTNFQTYNAIVKAVDDICFTVGERETVAVVGESGS
                     GKSVTSLSVMQLVPNPPGKIAGGRVLFHDSDLLALSERQMREIRGNKISMIFQEPMTS
                     LNPVHRIGDQIAEAIILHQNLDKKEAWDRAIELLRQIGIPSPEHRVMDYPHQLSGGMR
                     QRVMIAMALSCHPDLLIADEPTTALDVTIQAQILDLMRKMRNEMGMAILLITHDLAVV
                     AEMADRVLVMYAGRIVES"
     gene            complement(721..1608)
                     /gene="nikC"
                     /locus_tag="VF531_01945"
     CDS             complement(721..1608)
                     /gene="nikC"
                     /locus_tag="VF531_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEX7712759.1"
                     /translation="MAVELDTQTTIVKQAGEKPWHDFWRRFRRNRAAMVGLVILAVIL
                     VVSLFPGWFAPYDYKTNNMMESLQSPSFKHLLGTDEIGRDILSRIIYGAGLSLRVGVE
                     AVFLSLLIGIILGALAGYYGGFIDNFIMRIMDIMLAFPALLLAIAFMAVLGRGLDNAI
                     IAIGIVCIPEYARIVRGSVLSVKENEYVQAAKAIGNRDYQIILYHILPNVMAPIIVRA
                     TLGISGAILEAAGLGFLGLGVQPPEAEWGAMLGSGRSAIFSAPHIITFPGIAITITVL
                     SFNLLGDGLRDALDPRMKQ"
     gene            complement(1734..2738)
                     /locus_tag="VF531_01950"
     CDS             complement(1734..2738)
                     /locus_tag="VF531_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712760.1"
                     /translation="MLKYIIKRLLLLIPVLFGISLVVFIVMHLFTADPAALILGQHAT
                     VSQIQALRQEMGLNDPIYVQFGKFIWNLLHGDLGRSLMTRRPVVQEFISQFPATIELA
                     LFSLLLATVIGILAGIISAVKRYSIWDYTSMFGALLGVSMPIFWLGLILIIIFGVNLQ
                     WLPVAGRIDIGFEPATITRFYLLDSLLTGNWAAFWSTIRHLILPGVALAAYSMAIIAR
                     MTRSTMLEVVKQDYIRTAHAKGLSESRVIKHHALKNALIPVVTVIGLQLGFLLGGAML
                     TETVFSWPGIGSLTVNAIMASDYPMVQGSVLLVASVFVLVNLVVDLLYAYLDPRIRYS
                     "
     gene            complement(2841..4385)
                     /locus_tag="VF531_01955"
     CDS             complement(2841..4385)
                     /locus_tag="VF531_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712761.1"
                     /translation="MRKFVVVCLIVFTVFALVGVNFAAAPKILVYAKGSDPRGLDPAY
                     VDDGESAKIIVNIYDNLVKYKPGSTDVEPALATSWKQSADGLTWTFNLRKGVKFHDGT
                     PFNAEAVKFSVDRQLPPLATDDMPYASFTFDPNVIKEVKVVNTYTVQFQLKKPYAPFL
                     ANLAMSLSAPIVSPTAAKKWGKDFTQHPVGTGPFIFEKWDKDQQIVLRKNPTYWGVKA
                     KVDKVVFKVTKENSVRASELMTGAIDIMDGVDTNDVKTLEARKMNVIKNAGMNINYMS
                     FFVNKKPFNDVRVRRAVAMAIDRAQLVKYLYQGYAKLANGPLPSFIPGYDPNLKFVDY
                     NPAEAKKLLAEAGCPNLEFTLLTYSNPRPYNPATGVKLAEAIQADLLKIGVKTNIKVY
                     PWTEYKPVVFKAEEGDACFYGWIGDNGDADNFLSLLDGRQIDSSLNNAKYNNPVVNDL
                     LDKGTRTLDPKERIKIYQQLQKVLVEDVPWVYISHAVDMAATRPNVKGFSLHPTGVAW
                     LNTVTK"
     gene            4946..>5187
                     /locus_tag="VF531_01960"
     CDS             4946..>5187
                     /locus_tag="VF531_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712762.1"
                     /translation="MGQLVSLSPGGFQSLLISLIHIEENLKHFTEFSETPREADAVRK
                     LLEDYIMTLNRTIEQITVDEDSEDLFPYVVIGSEVT"
BASE COUNT         1339 a         1339 c         1269 g         1240 t
ORIGIN      
        1 ggattccacg atccggcctg cgtacatgac cagcactctg tcggccatct ccgctaccac
       61 cgccagatcg tgggtgatta acaggatggc catgcccatc tcgttacgca tcttgcgcat
      121 caaatcgaga atctgggctt gaatcgtcac atctaacgcc gtagtgggct cgtcggcaat
      181 cagtagatcg ggatggcagg aaagggccat ggcaatcatc acccgttgcc gcataccgcc
      241 ggataattga tgcggataat ccatcacccg gtgttccgga gaggggattc caatttggcg
      301 taacaattcg atcgcccggt cccaagcctc tttcttatcc aaattttgat gcaagatgat
      361 ggcttcggcg atctgatccc cgatacggtg aaccgggttt aaggatgtca tcggttcttg
      421 gaagatcatc gagatcttgt tgccgcgaat ctcccgcatt tgacgttcgg agagggcgag
      481 taaatccgag tcgtgaaaaa gtacccggcc gccggcgatc tttcccggcg gattgggaac
      541 cagttgcatc acggagagtg acgtaaccga cttaccgcta cccgactcac cgaccactgc
      601 cactgtctcc cgttccccga cggtaaaaca gatatcatcc accgctttaa cgatggcgtt
      661 ataggtctgg aaattggttt gtaaatgttc aacctttaat aactccatat tgagtcgctc
      721 ctattgtttc atccgcgggt cgagtgcatc gcgcaaacca tcacccagca ggttgaagga
      781 taaaacagtt attgtaatgg cgattcccgg gaaggtaatg atatgaggcg cggagaagat
      841 ggccgaacgg ccgctgccga gcatggcacc ccattccgcc tccggcggct ggactcccaa
      901 acccagaaag cctaaacccg ccgcctcgag gattgccccg gatatgccca gtgtggcccg
      961 gacgataatc ggcgccatga cattgggtaa tatgtgataa agaatgattt gataatcccg
     1021 gttgccgata gcctttgccg cctggacgta ctcattctcc ttgacggaaa gcaccgaccc
     1081 ccggacgatt cgggcgtatt ccgggatgca gacgataccg atggcgatga ttgcattgtc
     1141 caatcccctt cccagcactg ccataaaagc gatggccagt aagagtgctg gaaaggccag
     1201 catgatgtcc ataatccgca tgatgaagtt atcgataaaa ccgccgtaat atccggccaa
     1261 agctcccaga atgattccga tcaggagcga gagaaagacc gcctccactc ccacccggag
     1321 tgaaagcccg gccccataga taatccggct aagaatatcc cggccgatct catcggtacc
     1381 cagtaaatgt ttgaaactgg gcgattgcaa actctccatc atattgttgg tcttgtaatc
     1441 atacggagcg aaccaaccgg ggaaaaggga tactacaagg atgacggcca ggatgacgag
     1501 tcccaccatc gccgcacggt tacgccggaa acggcgccag aaatcgtgcc agggtttctc
     1561 ccccgcttgc tttactatag ttgtctgcgt atctagttca accgccatga caaaactctc
     1621 ctttatgcac gcgcatgcgc gaatcctgcc aagcgggtga ttgccggctc taccggttac
     1681 gacccgccgt cctcattaaa cctgttgtat ggtaatacgc ttatccaaaa agactacgag
     1741 tagcggatcc gtggatccag ataggcgtat agcaagtcga ccaccaaatt gaccaggaca
     1801 aatactgagg cgaccaaaag cacgctgcct tgaaccattg gataatccga tgccataatg
     1861 gcgtttacgg tcagactacc gatgccgggc caggaaaaga ccgtctcggt caacatggcg
     1921 ccgccgagca agaaaccaag ttgcaaaccg attaccgtaa ccaccgggat taacgcgttc
     1981 tttaatgcat gatgctttat gacacgcgac tcgctcaaac ctttggcatg agcggtgcgg
     2041 atataatctt gtttgacgac ctccagcatg gtggaacgag tcatccgggc gatgatggcc
     2101 atggaataag cggccagggc aactccaggt aaaatcagat gccggatggt actccaaaaa
     2161 gctgcccaat ttcccgtcag taaactgtct aagaggtaaa aacgggttat tgtcgccggt
     2221 tcaaagccga tatcgattcg ccccgccacc ggcagccatt gcaaattgac tccgaagatg
     2281 atgattaaaa ttaagccaag ccagaagatt ggcatggaga cacctaacag cgctccgaac
     2341 atactggtgt aatcccatat tgaataacgt ttcacagccg agatgatccc ggccaggatg
     2401 ccgatgactg ttgccaagag aagactaaac aaggccagtt cgatggttgc cgggaactgg
     2461 gagataaact cctgtaccac cggacggcgg gtcatgagtg agcggcccag atcaccatgg
     2521 agcagattcc agataaactt gccaaactgc acgtatattg gatcgtttag ccccatctcc
     2581 tggcggagtg cttgaatctg ggacactgtt gcatgttgcc ccagaatgag cgcagccgga
     2641 tccgcggtaa acagatgcat gacgatgaag acgaccaggc tgataccgaa caagaccggt
     2701 atcagtaata gcaaacgctt gataatgtat ttcaacatcg cttttcccgc cttgtatcaa
     2761 agaagtactg ccttactcgg gttatcgctc aatagaaggg gatgcgtaga tacgcatccc
     2821 cgataatcca ttacctcgat ttacttcgtg actgtattca accaagcaac gccggtcgga
     2881 tgcaggctga aacccttaac attaggccgg gtggcagcca tatccacggc gtggctgata
     2941 tacacccatg ggacatcctc gaccagtacc ttttggagct gttgataaat cttgattctt
     3001 tccttgggat cgagggttct ggtacccttg tccagaaggt cgttcacaac cggattgttg
     3061 tattttgcat tgttcaggct ggagtcgatc tgcctgccat cgagcaagga caggaagtta
     3121 tcggcatcac cgttatctcc gatccaacca tagaagcagg catcgccctc ttcggccttg
     3181 aagaccaccg gtttgtactc ggtccaggga taaaccttga tattggtctt taccccgatc
     3241 ttcaatagat cggcttggat tgcctccgcc agcttcacac cggtggccgg attgtaaggc
     3301 cgcgggtttg agtaggtcag cagggtgaat tccagattgg gacaaccggc ctcagccaac
     3361 aatttcttgg cttcggcagg attgtaatcc acgaatttaa gattgggatc gtaaccggga
     3421 atgaaactcg gcaacggacc atttgccagt ttggcgtaac cttgataaag atatttcacc
     3481 agttgtgccc gatcaatcgc catggccacg gcgcgacgga ctctgacgtc attgaagggt
     3541 ttcttgttga cgaagaagga catatagttg atattcatgc cggcgttttt gattacgttc
     3601 atcttccggg cttccaacgt cttaacgtcg ttggtgtcca ctccgtccat gatatcaatc
     3661 gcgccggtca ttaattcact ggcacggacc gagttctcct tggtgacctt gaagaccacc
     3721 ttgtcgactt tggctttcac tccccaataa gtggggtttt tcctcaaaac gatctgttgg
     3781 tccttatccc atttctcgaa gataaacggg ccggtgccca ccggatgctg ggtaaaatcc
     3841 ttaccccatt tcttcgcagc cgtggggctg acgatcggag cggaaaggga catggccaga
     3901 ttcgccagga acggggcgta cggcttcttt aactggaact gtacggtgta agtattcacg
     3961 actttgactt ccttgatcac attggggtca aaggtgaagg aagcgtaagg catatcatcg
     4021 gttgccaggg gcggtaactg acggtcaacg ctgaacttga ccgcctcggc gttgaacggg
     4081 gtgccgtcat ggaacttgac cccttttcgc aggttgaaag tccaggtcaa accatcggca
     4141 ctttgtttcc aggatgtggc caaagccggc tccacatcgg tgcttcccgg tttatacttg
     4201 accaggttgt cgtaaatgtt gacaatgatt ttggcggatt cgccgtcgtc aacatacgcc
     4261 gggtctaaac cccggggatc ggagcccttg gcgtagacca agatcttggg agccgcggca
     4321 aaattcactc cgaccagcgc aaaaacagta aacacaatca ggcataccac tacaaactta
     4381 cgcattgtct aacctcctct atttttttac ggttccaaac ttttatgaaa ccgtttggat
     4441 acattatgaa taaaaccgtt cgctcggcaa ttcaaaaaat cctgaaaagg gcgcacggtt
     4501 tatcaacatt cagcgggcaa agtcatagtt gataatgaat aaatattaaa tttgacttaa
     4561 aatcatgcaa gaattgcttt atttctcatc taaaatagca tttcgcaagt aaccgtgtca
     4621 aggaaatcgg ccaagaatat tgtatacatg caagaatact gtatacatat ccggccatgc
     4681 gacgacagcg ccactttgct aaaaaatgac gtgggatacc tggcatttcc tgcgatttat
     4741 tcatggcaca ttttatgata taatcattcc attcaaaaaa taatgccatt attgaagtca
     4801 accgacaatt tccaaattct ctcggcaaaa taatgaaaag ctccggatta ttcattggcc
     4861 atccgcgaca caatgaatcc ggcaaattct tttgccgcgt tgtttccgtt actttccgga
     4921 ttcatattga aaagagggaa atgaaatggg ccaactcgta tctttatcgc cgggcggctt
     4981 tcaatcattg ctcattagcc tgatccacat cgaggaaaat ttaaaacatt tcactgaatt
     5041 ctcggaaact ccccgggagg cggatgccgt acgcaaattg ttggaagatt atatcatgac
     5101 cctcaaccgt accatagaac agattacggt ggatgaggac tccgaggatc tctttccgta
     5161 cgtggtcatc ggcagtgagg tcacgat
//
LOCUS       DASVMB010000058         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_5444,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000058 DASVMB010000000
VERSION     DASVMB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5444"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VF531_01965"
     CDS             complement(<1..536)
                     /locus_tag="VF531_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX7712763.1"
                     /translation="MAVKVGINGFGRIGRNAFRAMQNHSDLEIVAVNDLTNPKTLAHL
                     LKYDSVHGRFNGDIKPTETGILVNGREIKVLSVKDPAELPWKDLGVEIVIESTGLFTV
                     KKDGVNKKGKQVKGAENHITHGGAKKVIISAPAEGEDITIVMGVNEDKYDPRNHHVIS
                     NASCTTNCLAPFAKVLHGR"
     gene            complement(653..1684)
                     /locus_tag="VF531_01970"
     CDS             complement(653..1684)
                     /locus_tag="VF531_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEX7712764.1"
                     /translation="MDKLLTLQQKIVPEIFPLLLSRYQVLRAIRFSQPIGRRALAERL
                     HFQERKIRNEVDLLKSQELIICSAMGMEVTPEGDVLLWALEDYIREIQGLSLLEQKIA
                     ELFKVKKVMVVPGDTDQDDSVKKNLARVTAEYLLHHLTNGSILAVTGGTTLAEVAGAL
                     TVPAAKRDITVIPARGGLGEEVEIQANAVAANIAKGLGASYRLLHAPDDLQAKTMQSI
                     VDEPKIREILDLLRRADMVLHGIGSAEEMARRRETPSGKITELLGQGAVGEAFGYYFA
                     LDGSVIYSTSSIGLQLNELSLIDQVVAVAGGKSKAKAVIAVLSNAPRDVLIIDEGAAR
                     AVVAMTSSG"
     gene            complement(1809..2786)
                     /gene="whiA"
                     /locus_tag="VF531_01975"
     CDS             complement(1809..2786)
                     /gene="whiA"
                     /locus_tag="VF531_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX7712765.1"
                     /translation="MQKFSQTVKDEVVREELGTARECIKAELAALLHMTGSIHLTGQQ
                     QLSLNLMIETAGVARRVVKLLKASYNLESEVRVEQFERLGKPHRYNLIIPAQKGLREM
                     LAELGMLTREHQIESGIKPELVKDNPCRAAFLRGAFLGGGSITDPQKKTYHLELVTHS
                     EDFANGLAYLMNLLGLKAKISQRKEHFLVYLKEGEALAKFLSLISANNAVLKLEEVRV
                     IKELRGQVNRRVNCETANLQKTLDAAWEQVEMITALKNGGKLANLPANLRETAELRLE
                     YPEASLKELGEYHKPPLSKSTVNHRLRMIRECTKKFYVTAGIHSGKEKK"
BASE COUNT          782 a          853 c          712 g          870 t
ORIGIN      
        1 cgcccgtgca gcactttggc aaaaggcgcc aaacagttgg ttgtacatga ggcattggaa
       61 attacatgat ggttgcgggg atcatattta tcttcattaa cccccataac gatggtaata
      121 tcttcacctt ctgccggagc cgagatgatt accttcttgg ctccgccatg ggtgatatgg
      181 ttctcggccc cttttacctg tttgcctttt ttattgacac cgtctttttt gactgtaaat
      241 aacccggtgg attcaatgac gatttcgacc cccagatcct tccagggaag ctccgcaggg
      301 tctttgaccg ataatacctt aatctcccgc ccgttcacca aaatcccggt ctcagtcggc
      361 ttgatgtcgc cgttaaaccg gccatgtacc gagtcatatt tgagaagatg ggcaagagtc
      421 ttgggattgg taagatcatt gaccgcgacg atctccagat cggaatggtt ctgcatcgcc
      481 cggaaggcat tcctcccgat gcgaccgaaa ccgttaatac ctacttttac cgccatttac
      541 taacctccac ttcactttat tcgtagggat atgtggatca cggtgtccgg cggcagactc
      601 ggaaatccgt tacgatggga ttggggcgga tatgatttgc ctataccgaa aattacccgg
      661 aagatgtcat ggccaccacg gcccgtgccg ccccttcatc gatgatcaga acgtcccggg
      721 gcgcattgga gagtactgca ataacggcct tcgctttact cttgccgcct gccacggcga
      781 ccacctgatc gatcaggctt agttcattta gttgcagacc tatgctggag gtggagtaga
      841 ttaccgagcc gtccaacgca aaatagtacc cgaaggcttc acccaccgca ccctgaccta
      901 gaagttccgt tatcttacct gaaggggtct ctcgccgccg cgccatttct tcggccgatc
      961 caattccatg gagtaccata tcggcacggc gtaacaaatc taaaatttcc cgaatcttcg
     1021 gctcgtcgac gatggactgc atcgttttgg cctgcaggtc atcgggagcg tggagtaacc
     1081 gatagctggc tccaagtccc ttagcgatgt tagccgctac tgcattcgcc tgaatctcga
     1141 cctcttcccc cagacccccg cgagccggta tcactgtaat gtcccgttta gccgcgggca
     1201 ccgtcaacgc tccggcgact tcagctaagg tggtaccgcc cgtaactgcc aatatggaac
     1261 cgttggtcaa atgatgcaat aagtattccg cggtgaccct ggccagattc ttcttgaccg
     1321 aatcgtcctg atccgtatcc cccggtacta ccatcacctt tttaacctta aataattcag
     1381 caattttttg ttctaataaa ctgagcccct gaatctcccg gatataatct tcaagcgccc
     1441 agaggagtac atctccttcc ggggtaacct ccattcccat agccgaacaa ataatcaatt
     1501 cctgactttt taggagatca acctcgttac ggatcttccg ttcctgaaag tgcaaacgtt
     1561 cggccaaggc ccgccttccg atgggttgag aaaaacggat ggcgcgtaaa acttgatatc
     1621 gcgagagaag taaggggaaa atctcaggaa cgattttctg ttgtaaagtt aaaagtttat
     1681 ccatgatttg ctctgattga attcgtccaa attttagggc ttgtagcagc tggataattt
     1741 ttgtcccagc attatataat atgtcccgct acagcaaaaa aataaatacc ttaccatgat
     1801 gattccaatt atttcttctc tttcccacta tgaattcctg ctgtgacgta gaattttttg
     1861 gtgcactccc ggatcatgcg tagtcgatga ttgacagtgg atttactgag cggcggttta
     1921 tggtactcac caagttcctt caaggaagcc tccgggtatt ccagacgcaa ctcggcggtt
     1981 tcccggagat tggccggtaa attagctagt tttcccccgt ttttcaaggc ggtaatcatt
     2041 tctacctgtt cccaggcggc atccagtgtc ttttgaagat tggcagtctc acaatttacg
     2101 cggcggttga cttgaccccg taattccttg atcacccgca cttcctccaa tttgagtacc
     2161 gcattattgg cacttatcaa tgacagaaac ttcgcgagag cctctccttc ctttaaatag
     2221 accaaaaaat gctcttttct ctggctgatt ttcgccttga gtcccagtaa attcatgaga
     2281 taggccaaac cgttggcaaa atcctcgcta tgagtcacca actccaggtg ataggttttc
     2341 ttttggggat ccgtgatcga gccgcccccc aaaaaagctc cacgcaagaa agctgcccgg
     2401 caaggattat ccttaaccaa ttccggtttg attccgcttt caatctggtg ttcccgggta
     2461 agcatcccga gctccgccaa catctcccga agtccttttt gggcggggat gatcaggttg
     2521 taacggtgag gtttaccaag ccgctcaaat tgttcgaccc taacttccga ttccagattg
     2581 taagaggctt tgagtagctt tacaacccgt cgcgccacac cagccgtctc gatcatcaaa
     2641 ttcaacgaga gttgctgctg cccggtgaga tggatgctac ccgtcatatg cagcaatgcg
     2701 gccaattcgg ccttgataca ttcccgggcc gtacccaatt cctcccggac gacctcatct
     2761 ttaactgttt gagagaattt ttgcacaggc catcaccccc aatataggca atagggaata
     2821 ggcaacaggc aatggaatac gtatttcttt gaagacaatg tactagtcaa aaagggaaca
     2881 gtgaatctag gttcaatagc ccgaaatctg acccacctca ccgaccttct tcaaaacaag
     2941 ttcaaagcag agtgaaataa cagcagagat caggatcaca ctctcacgtg tgcgtgattt
     3001 caatatttgc actacagagg gtgagaatga gccatagggg gtcaaatcgc ctaacttcag
     3061 tactgttgcc tgttacctag tgcctattgc ctcatgcatt atgccttttc tctgttaatg
     3121 atgttttttt cgccaatcat tcataaaaat gaactttccg gcttaatatc aaatgaacac
     3181 cgggcatccg ggcctctagg aaggtgattt tgatgat
//
LOCUS       DASVMB010000059         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_152903,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000059 DASVMB010000000
VERSION     DASVMB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152903"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /gene="gndA"
                     /locus_tag="VF531_01980"
     CDS             <1..984
                     /gene="gndA"
                     /locus_tag="VF531_01980"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX7712766.1"
                     /translation="PYFTAISAHVNQEPCCRFLGPDGAGHFVKMVHNGIEYADMQLIS
                     EAYLLLRELGGMSPAELQQIFTEWNQGDLNSYLIEITAAILGKTDADTEKPMVDMIMD
                     RAGQKGTGRWTVQEALELGTAIPAVAEAVFARCLSTLKEERVQAAKELTGPAVKPEGD
                     RKALIQAVQEALYASKICSYAQGFAMLGVASQNYHWQLDLGEIALLWRGGCIIRAQFL
                     NRIKEAYAQNRDLAHLMLADYFKERLIKADESWRRVVGLAVANGLSVPVFSAALSYYD
                     SYRRSVLPANLIQAQRDYFGAHTYERVDRTGIYHSEWEVPEKRITSVKADA"
     gene            1065..2501
                     /locus_tag="VF531_01985"
     CDS             1065..2501
                     /locus_tag="VF531_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX7712767.1"
                     /translation="MNVVGLEVSTSAAKAVLFSRGEGVLATSTIPFDGAVSDTVSQDP
                     DGVVEAALQALAEITLHSREPIAGIGLGGTWHSLLLLDKEYRPLERVHTWADLSASSL
                     VSEVRRDSKLVEWFYQRTGCMVHAMYPLWKYLDIKRRRPELVEKTAYLGSSLEYLFMR
                     LTGRQCLSKCLASGSGWFNIRRLDWDSEILDYAGVDPGQLGELKELPEWAPLTVEAAA
                     RTGLLAGIPVTVGGADGAMNQIGIGGVIPGIMSFSVGTSGALRITQSEPKLPVTPSTW
                     CYYLYDGKRLAGAATHACSNLDWFLSNWNSAKGKGYDQYAQEAALIERENAPYFLPFI
                     YGERCPGWREDRSGGFLELRAGHRRADLYYAVLEGILFNLYQCYRSLTATCEAAAEIR
                     ISGGIMNSPFWLQLAADIFGREITATSVTNDSTVGAALFALEASGGLADVAEYRPALT
                     DTVQPRAEFRELLERRFQHYLTLYQANG"
     gene            2517..>3448
                     /locus_tag="VF531_01990"
     CDS             2517..>3448
                     /locus_tag="VF531_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX7712768.1"
                     /translation="MQEITVPQLEDSLRKWLENYHGLRRVLLLPPDITRYYSRAGLIV
                     QLLYKMLAPECRVDIMPALGTHLPVNESERLKMFGDIPAGNFFVHDWRNDTVKIGTVP
                     EGYVAEVSDGLVRYPIPVMVNRRLLEREYDLIVSIGQVVPHEVVGMANFTKNILIGCG
                     GKEFIDKSHFLGAAYGMERLMGRDHSPVRKVFDYAENNFLQGLPLQYMLTVLSAGGAL
                     CGFFAGRSRQLFEAAVDLSRRENLTMLDRSLNRVVVYLEPEEFKSTWLGNKAVYRTRM
                     AIADGGELLVIAPGLRQFGEDREIDGLIRKYGYVG"
BASE COUNT          768 a          821 c         1041 g          818 t
ORIGIN      
        1 ccttatttca ccgctatctc agcccatgta aatcaagagc cgtgttgccg gttccttgga
       61 ccggatgggg cggggcactt cgtaaagatg gtgcataacg ggattgagta cgcagatatg
      121 cagttgatca gcgaggctta cctgttgctt cgggagttgg ggggcatgag tcccgccgag
      181 ttgcaacaga tctttaccga atggaatcaa ggcgatctga attcctacct aatcgagatc
      241 accgccgcca tccttggaaa gacggatgcc gataccgaaa aaccgatggt ggatatgatt
      301 atggaccggg ccggccaaaa agggacgggc cgctggactg ttcaggaggc tctggaattg
      361 ggaacggcga ttccggctgt ggccgaggcg gtctttgcac gctgcttatc tacattaaaa
      421 gaagaaaggg ttcaggccgc aaaggaactg accggaccgg cggtaaagcc cgaaggcgat
      481 cgtaaggcat tgatccaagc ggttcaggaa gctctatacg cctcaaagat ttgctcctat
      541 gcccagggtt ttgcgatgct cggcgtggct tctcaaaatt accactggca gcttgatctg
      601 ggagagattg cccttttatg gcgtggtggt tgtatcatca gggctcagtt tttaaaccgg
      661 atcaaggagg cctacgcgca aaaccgggat ctggcccatc tgatgttagc ggattatttt
      721 aaagagcggc tgattaaagc ggatgaaagt tggcgccggg tggtgggact ggctgtagcg
      781 aacggattgt cggtgccggt tttcagcgcg gctttgagtt actatgattc ctaccgccgt
      841 agtgtgctcc ccgccaatct gatccaagcc caacgggatt attttggcgc ccacacctat
      901 gaacgggtcg atcggaccgg gatttaccac agtgaatggg aggttccgga gaagcggatc
      961 acaagtgtta aggcggatgc ctaaaatgat cgacccggtt gacccttaag tgagaatgaa
     1021 ccgtgtttgg ttgaaaaaaa atttgtaaac taggagtcgg taccatgaat gttgtggggc
     1081 ttgaggtcag tacttcggcg gcaaaagcgg tcctcttttc acgcggggaa ggagtgctcg
     1141 ccaccagcac gataccgttt gacggggctg tttccgatac cgtttcccag gatcccgatg
     1201 gcgtggtcga agcggcatta caagcactcg ccgagataac cctgcacagc cgggaaccga
     1261 ttgccgggat agggctgggc ggtacctggc atagtttgtt gttgctcgac aaagagtatc
     1321 gtccgctgga gcgggttcac acttgggccg atctcagcgc cagctcactg gttagtgagg
     1381 ttcgccgtga ttccaaactt gtcgaatggt tttaccagcg aaccgggtgt atggtacatg
     1441 ccatgtatcc gctgtggaaa tatcttgata tcaaacggcg ccggccggag ttggtcgaaa
     1501 aaactgctta cctgggatcg agcttggaat atctgtttat gaggcttacc ggacgccagt
     1561 gtctctccaa atgtctcgct tcgggaagcg gctggttcaa tatccggcgg ctggattggg
     1621 attcggagat tctggattat gcgggggttg accccggaca actcggggag ttgaaggaac
     1681 tgccggagtg ggcgccgctg acggtggagg ccgccgcccg caccggtttg cttgcaggta
     1741 ttccggttac ggttggaggt gccgatggtg ccatgaacca aatcggaatc ggcggggtga
     1801 tccccggtat catgtcgttc tccgtaggaa ccagcggtgc cttgcggata acccaatccg
     1861 agccgaaact gcctgttacc ccatccacct ggtgttatta tctttacgac gggaagcgct
     1921 tagccggggc ggctactcat gcctgctcca atctggactg gtttttaagc aattggaaca
     1981 gtgccaaagg taagggatat gatcaatatg cacaggaagc ggccttgatt gaacgggaaa
     2041 acgccccgta ttttctcccc tttatttacg gggaacggtg tcccggatgg cgtgaagacc
     2101 ggagcggagg ttttctggag ttgcgcgcag gccatcgccg ggcggacctt tattatgcgg
     2161 tacttgaggg aatcctgttc aatctgtatc aatgctaccg gagcttaacc gctacttgcg
     2221 aagcagccgc ggagatccgg atatcggggg gaatcatgaa ttcccccttc tggctgcagc
     2281 tggctgcgga tatctttgga cgggaaatta ccgcaaccag cgttaccaat gattctaccg
     2341 taggcgccgc gctcttcgcc cttgaggctt ccggaggtct ggccgatgtt gctgagtacc
     2401 gcccggcgtt aacggatacg gttcaacccc gtgcggagtt ccgagaatta ttggagcgaa
     2461 gattccagca ttacctaacg ttatatcaag caaatggata aaaaggaggg aaaagcgtgc
     2521 aggagatcac cgttccgcaa ttggaagatt cgctacgaaa gtggcttgaa aactaccatg
     2581 ggttaagaag ggttttactc ttgccgcccg atatcacaag atattactca cgggcgggac
     2641 tgatcgtgca gctgttatac aagatgttgg ccccggaatg ccgggtcgat attatgcctg
     2701 ccttggggac acaccttccg gtgaacgagt cggaacggct caaaatgttt ggggatatcc
     2761 cggcggggaa tttttttgtc cacgattggc gcaatgatac tgtaaagatc gggacagttc
     2821 ccgaagggta tgtggctgaa gtttcggacg gactggtccg ctacccgatc ccggtcatgg
     2881 tgaaccgccg tttattggaa cgggaatatg acctgatcgt atccatcggc caggtggtac
     2941 cccacgaagt ggtgggaatg gccaatttca ccaagaatat cttaatcggt tgcggaggca
     3001 aggagttcat cgataaatcg cattttttgg gagccgctta tggcatggaa cggctgatgg
     3061 gacgggatca ttccccggta cgaaaagtct ttgattatgc cgaaaacaat tttctccagg
     3121 gtttgccgct ccagtacatg ctgacggtat tgagcgccgg aggagcctta tgcggtttct
     3181 tcgccggccg ctcccggcag ctttttgaag ctgctgtcga cctgagtcgg cgggagaacc
     3241 tgaccatgct cgaccggtct ctgaatcggg ttgtcgttta tcttgaacct gaggagttta
     3301 aaagcacctg gctcgggaat aaagctgtct accgcacccg gatggcgatt gccgacggcg
     3361 gggaattgct ggtcattgcc cccggtctcc ggcaattcgg agaggatagg gagattgacg
     3421 gattgattcg taagtacgga tatgtagg
//
LOCUS       DASVMB010000060         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_522353,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000060 DASVMB010000000
VERSION     DASVMB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522353"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF531_01995"
     CDS             complement(<1..214)
                     /locus_tag="VF531_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712769.1"
                     /translation="MPIEDPIVPNQSVKKGPSLPINLGVLGMILIILTILALTLGSAR
                     LSVFQVGRVILEPLTAIGLPVPGTPDP"
     gene            complement(234..1133)
                     /locus_tag="VF531_02000"
     CDS             complement(234..1133)
                     /locus_tag="VF531_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX7712770.1"
                     /translation="MKINKLRFIPAILLTLIVLCTAFSPIIGAATQFPLTMKDQFGRE
                     ITLTKAPQRIVSGSPGNTEILFALGLEKRIVGVTDWCNYPEPAKKLSKIGDISPMNIE
                     RVLALRPDLVVADVMNGKESVDRLSELGLPVLALNPNSFQDILTAIRFIGQATGKTQA
                     AEELVKQLDATLEQVRRQGTTVRKRGLKVFIITNWDPCWTAGPGSFLDEAVKLAGGIN
                     IAHDLNKPWGQLSMEAVIARNPDLVITDVDPAKIYSDPNWASLAAVRKHQVYKIIGDE
                     YYRPGPRLIMALRDLAALLERSK"
     regulatory      complement(1320..1531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1670..2191)
                     /gene="dcd"
                     /locus_tag="VF531_02005"
     CDS             complement(1670..2191)
                     /gene="dcd"
                     /locus_tag="VF531_02005"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX7712771.1"
                     /translation="MILSGKEIRNRLGRDIVIEPFNEEQLNPNSYNLRLHNELLVYEK
                     SLLDMKQENPTRKLVIPPEGLILESNRLYLGRTLEYTKTDRLVPMLEGRSSIGRLGLF
                     VHVTAGFGDVGFCGYWTLEIFCIQPVRVYAGVAICQIYYHTIEGDYDRYSCGKYQNNT
                     GVQPSLLFREFQK"
     gene            complement(2433..3104)
                     /locus_tag="VF531_02010"
     CDS             complement(2433..3104)
                     /locus_tag="VF531_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7712772.1"
                     /translation="MRKIKRTNYDIYLFDLDGTLTDSQPGIIHSVQYALEKLGIIESD
                     LQKLLPFIGPPLAESFREFYGFDAASALQAVEFYREYFREKGIFENNVYEGIPALLAE
                     LRQSDYTLAVATSKPTIFTDRILNHFQMRNFFEVVIGSNLDGTHSSKAEVIGLVLGKI
                     KDPSQKAVMIGDRKHDILGAQENAIDSIAVAYGYGTVTELEAAKPTYLVRSVAELQEL
                     LVGEG"
     gene            3384..>3853
                     /locus_tag="VF531_02015"
     CDS             3384..>3853
                     /locus_tag="VF531_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712773.1"
                     /translation="MLKRYMAKMTVAAVFLLLFSSRFVDVTIGEKLHDYPLESAWKAT
                     GIPLGAVDTAVWMKLNDRWFSVRQLTAKAKELQNALKLKNCSELISGEQAEFTFASLQ
                     GTRSDGTVVTITLQSSSSVEANETQLGINTSYSVGACGAISDLNRYLETLRADLG"
BASE COUNT          923 a          965 c          985 g          980 t
ORIGIN      
        1 tggggtccgg cgttcccggt accggcagac ctatggcagt caacggctcc aggatgaccc
       61 gaccgacttg gaacacgcta agccgggcgg atcccagggt taaagccaga atcgtcaaga
      121 ttattagaat catccccaaa acgccgagat tgatcggcag ggatggacct tttttgacag
      181 actgatttgg tacaatgggg tcctcgatgg gcatctatac ctccgttaac gggtcatttg
      241 ctccgttcca aaagcgcggc cagatcgcgc agggccatga tcagccgggg gccgggccgg
      301 tagtattcat ctccgataat cttgtaaacc tgatgcttgc ggactgcggc gagggatgcc
      361 caattggggt cggaataaat cttggcggga tccacgtcgg tgatcactag atcggggtta
      421 cgggcgataa ccgcttccat actcaactga ccccaaggct tgtttaggtc atgggcaata
      481 ttgatcccgc ctgccagttt gactgcttca tccaggaaag agccgggtcc tgcggtccag
      541 caggggtccc aattggtaat gataaatact tttaaccctc gctttctcac cgtagttcct
      601 tggcggcgga cttgttccag ggtggcgtcg agttgtttga ccagttcttc ggcagcttga
      661 gtttttccgg tcgcttgtcc gatgaaacga atggcggtga gaatatcctg aaaggaattg
      721 gggttgagcg ccagtaccgg aaggcccaac tcactcaggc gatcgacgga ttccttgccg
      781 ttcatcacat ccgccaccac tagatccggc cggagcgcca gtacccgttc aatattcatc
      841 gggctgatat cgccgatttt ggaaagcttt ttggccggct ccggatagtt gcaccagtcg
      901 gtgacgccga caatcctttt ctccagtccc aaggcaaata agatttcggt attgcccggt
      961 gaaccggaga cgatacgttg gggtgccttg gtgagggtta tctcccgacc gaattgatcc
     1021 ttcatggtga gtggaaattg cgtcgctgcg ccgatgatgg gactgaatgc ggtgcacagt
     1081 acgatcagtg taagtaaaat ggccgggata aaccgtaatt tattgatctt catttacgta
     1141 acctcctcaa tttgatctag ggagttcact taaaaaccat ataagtagct cataaacaac
     1201 gaagcagttc tctgatacat aaaataaaaa acccggttcc gctgatacgg gaaccgggat
     1261 ggatcgttgc gataacaagt gtggccaagt cggtttcacc atcccctttc cgcgaaggaa
     1321 cgtgatacaa gaataagggc aggtttcctg acttccggat caacatacaa cgttagctca
     1381 gccttccggc atcagcacac gtcttattaa aaaccgtgac tgaggcagtg gcatgagctg
     1441 tattccccgg ttacagtggc gggaccgttc aggcattcca cctgattccc tattatcccc
     1501 ggtttggggc accctcttcc atatggatat tcaattttca aggacgggta aaaatgtgct
     1561 ttaccagggc cggtcgaaac ggaatagagg ccggtggctg gaagccacga actactctta
     1621 ttgtaaatgt tttcatgcat ttaagcaaga cctggccgtt ttgaaacgtt tatttttgaa
     1681 actcccggaa cagcagactg ggctggactc cggtattatt ctggtattta ccgcagctat
     1741 atctgtcgta atctccctcg atggtatgat agtaaatttg gcagatggcc accccggcgt
     1801 agacccgaac cggttggata caaaagatct caagggtcca gtatccgcaa aaaccaacat
     1861 cgccgaaacc tgccgtgaca tgaacgaaaa gacccaaccg gccgatggag gaacgaccct
     1921 ccaacatcgg gaccagccgg tcggtcttgg tgtattcgag ggtccttccc agatagagcc
     1981 ggttggactc aagtatcagt ccctccggag gaatgacgag cttccgggtg gggttttcct
     2041 gtttcatatc gaggagtgac ttttcataga ccagaagttc gttatggagc cggagattat
     2101 aactgttggg gttgagttgc tcctcgttaa aaggttcgat aacgatatcc cggcccagcc
     2161 ggttacgaat ctctttgccc gataagatca ttggggaacc tccatccaaa gtcgagaatt
     2221 aaattaggca acaggcaacg ggcacgaggc gagagtattg caggttattt attctggtcc
     2281 aaagtccgaa gtttgaaacc tgctaacgct tgattattgc gtttttaacg tgcatgccaa
     2341 gtaggccttc ttaagggacg gggtcgcctt taggcccaaa ccaattctcc aggctctctg
     2401 ttgcctattg cctgttccct catgcctcat gcctatccct caccaaccag caactcctgc
     2461 agttcagcca cgctccttac caagtaggtg ggtttcgccg cttcaagttc ggtcacggtt
     2521 ccgtatccat aagccacggc gatgctatca atggcgtttt cctgagctcc caggatatca
     2581 tgcttgcgat caccgatcat caccgctttt tgagaaggat cttttatttt cccgagcacc
     2641 aggccaatga cctcggcctt ggaagaatgg gtgccgtcaa gattgctccc gattacaacc
     2701 tcaaaaaaat tacgcatctg aaaatggttt aaaattcgat ccgtaaagat tgtgggtttg
     2761 gaagttgcga ctgccagtgt ataatcgctt tggcgcagtt cggcgaggag ggcggggatc
     2821 ccctcataga cgttattctc aaatatcccc ttctcacgga aatattcccg gtaaaattct
     2881 accgcctgaa gggcgctggc tgcgtcgaaa ccataaaatt cgcggaatga ctcggctaag
     2941 ggcggaccga tgaatgggag caatttctgg agatcggact cgatgatgcc caatttttcc
     3001 agggcgtatt gcaccgaatg gatgatgccg ggttgggaat cggtcagggt accgtccaga
     3061 tcaaacagat agatgtcata attggttcgt ttgattttcc tcaactactt tctattcact
     3121 tgatttactc atactcgttg ccttttccct ggtgcctgtt gccccgcgcc cgcagggtgc
     3181 gatgacagag ttaggtattc ccgctccgca ctttactttt ccaatttctt tctccaaatt
     3241 tcggcgactt tcaggaattc ccttcttaaa ttcccgatga agtaacaaac cccgtccttg
     3301 aaggtataga tgggcttggt tccgccgaaa atgttggggt aagactttta tcccagcatt
     3361 cagaagcttc gggaggaatc cggatgctca aacgctatat ggcgaagatg actgtagccg
     3421 cagttttttt actgcttttc tcatccagat tcgtggatgt aaccatcggc gaaaaactcc
     3481 atgattatcc gttagagagt gcctggaagg caacgggtat ccccttgggc gcggtggaca
     3541 ccgctgtctg gatgaagctt aacgacagat ggttcagcgt ccgtcagctc acggccaaag
     3601 ccaaagagct tcaaaacgca ttgaaactca agaactgttc ggaattgatc tctggggaac
     3661 aagccgagtt cacttttgca tctcttcaag gtacgcgctc cgatggaact gttgtgacga
     3721 taacgttaca gtcaagcagt tccgtggagg caaacgagac ccaattaggg atcaatacca
     3781 gttattcggt aggcgcctgc ggggcaatat cagatctcaa ccgttatctg gagaccttac
     3841 gggccgatct cgg
//
LOCUS       DASVMB010000061         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_300561,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000061 DASVMB010000000
VERSION     DASVMB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300561"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VF531_02020"
     CDS             complement(<1..1650)
                     /locus_tag="VF531_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX7712774.1"
                     /translation="MNFKLHCGFLWIALFFCIILSGCGGGPKTGSNPDPGPGTLNVPA
                     ETVTKVLSDTDTNLAPLFQSDTQPSTEAIRDKLAAISDLHIKTTTDDGVVVAEIGGAG
                     GPLIIVAPPTPVDDSFVPASVPVSQTSRSMVMAASSYSSLPKDSKVSLLTGFDGTTIY
                     KDVTPKIQQIFAGCSSGYTVTTQQATIENLRNVKGIDLLFIKTHGERIASIAPYPNQG
                     IETYCLSSITRVDKKNDPFPKAYATDILKHNIVYMWIPTDRTGILFKDFKYEWVFGIT
                     AGFVTEYWQFNKGGMAFISACSSANPAAGPFQQSVFDSGVSTYFGWDQQIDTNFADDR
                     AQFVFDRLLGANVFVNDTPLVRAFPYQDVYKEMVTKGMVTWPGDDKHVASTLKVFDNP
                     ADDNFGILEPSIKYVEVDEKNQEPSFPAVLTLYGVFGREEGKVTVGGTELSIISWSED
                     QITCRITDQPNGAGAAGDVVVDVRGHKSNTVPLTLWKLIYTYTMKGDGTLQDYLTVTC
                     YLRGDIHRYRETPGGDPIDGRMVQLSNMRGGLTGNYRFSGQY"
     gene            complement(1812..>2996)
                     /locus_tag="VF531_02025"
     CDS             complement(1812..>2996)
                     /locus_tag="VF531_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712775.1"
                     /translation="DVAKRIEVDSLRVEGIDVVEVNYEYDLVDKQRLLGKYLGETVLL
                     VEVETNNKRACRLLNPVELILEDIQTGEVLVEPAGELVLPKLPQGLSARPALIFRLRP
                     SHATGMRISYITNGLSWSTHYVAELQGDHLGLDAWVELRNESGITFHGARLKLLAGEV
                     NRVREMLRADYCLPMSPAEEKTAPDFEEKGFSDYHLYPLQGVTSLKDNQTKQIRLFDA
                     EKVPYRRYYEIAPYSEDARIIVEFTNQNQSGLGVPFPAGKFKFYQRDEADHGLTFIGE
                     DEIGHTPKDEAIKLCIGEAFDLKCERTCLNKQNNLGVRKESWQITLKNRKEESALVRV
                     LHRVPGTGEIVEADHHWTREMADMIMFEMKLDAGTVENIKFAVIIDERVHVIKHEPSP
                     PG"
BASE COUNT          744 a          833 c          679 g          740 t
ORIGIN      
        1 atactgtccg gaaaatctat agtttccagt taatccgcca cgcatatttg agagttgtac
       61 cattcgtcca tcgatcggat ccccgcccgg ggtttcccgg taacggtgaa tatcgccccg
      121 gagataacag gttacggtta aataatcctg taacgtacca tcgcccttca tggtataggt
      181 gtatatcaat ttccagaggg ttagcggaac ggtgttactc ttatgtcctc ttacatccac
      241 cactacatcc ccggccgctc cggccccatt cggctgatca gtaatccggc acgtaatctg
      301 atcttcactc caggaaatga tggatagctc agtcccgcca accgtaactt tcccctcttc
      361 tctcccaaaa accccataga gcgtcaacac cgcaggaaac gatggctctt gatttttttc
      421 atccacttcc acgtatttga tactgggttc caggattcca aagttgtcat ccgccggatt
      481 atcgaagacc tttaaggttg atgccacatg cttatcatcc ccgggccaag ttaccatacc
      541 tttcgtgacc atctctttat acacatcctg gtatggaaag gcccgcacga gcggagtatc
      601 attaacaaaa acattggccc ccaacaaccg gtcaaataca aattgggccc ggtcatcggc
      661 aaaattggta tcgatctgtt gatcccagcc aaagtaggtc gataccccgc tatcgaaaac
      721 gctctgctgg aaaggcccgg ccgccgggtt cgcactggaa catgcgctaa taaaggccat
      781 cccaccctta ttaaactgcc aatactccgt aacaaatccg gcagttatac caaataccca
      841 ctcatacttg aaatctttga ataaaatgcc ggtgcgatcc gttggaatcc acatataaac
      901 gatattatgc ttcagaatat ccgtagcata ggccttggga aaagggtcat tttttttatc
      961 cacccgcgtg atcgacgaca gacaatacgt ttcgatccct tgatttgggt agggtgcaat
     1021 ggacgcaatc ctttccccgt gagtcttaat aaaaagcaga tcaatccctt tgacattccg
     1081 cagattttcg atggtcgcct gttgcgtggt aacggtgtag ccactgctac aacctgcgaa
     1141 tatttgttga atcttcggcg ttacatcctt gtaaatggtg gtgccgtcaa aaccggtcaa
     1201 aagggatact tttgaatcct tgggaagaga gctatatgag cttgcagcca tcaccatgga
     1261 tcggctggtt tgactcaccg gaacgctggc cggaacaaag gaatcatcta ccggagtagg
     1321 aggggcaact atgatgaggg gtccacccgc cccgccgatc tcagccacaa cgactccatc
     1381 atcggttgtg gttttgatat gtaagtcgga aatagcggcc aatttatccc gaatcgcttc
     1441 ggtggatggt tgggtatcgg actgaaagag cggcgccagg ttggtatcgg tgtcgcttaa
     1501 aactttcgtg actgtttctg ccggaacatt tagagtcccc ggcccgggat cgggattgga
     1561 acccgttttt ggtccaccgc cgcatccaga caagatgatg caaaagaaga gtgctatcca
     1621 taaaaaaccg caatgtagtt tgaaattcat cggcctcatc cttccaataa gaatctcttg
     1681 atcctcaata taattataaa aaaagcctct gaaccaaaag tgaacaaacc cagccacccg
     1741 cggtttcttt ggataaaaaa ttccggcaac taatacaact ctactttagt gggagagggc
     1801 cggcccggat gttagccggg gggtgagggc tcatgcttga tcacatgcac cctttcatca
     1861 attattaccg caaattttat gttctccacc gtacccgcat cgagcttcat ttcaaacata
     1921 atcatatccg ccatctcccg ggtccaatga tgatcggcct ccactatctc accggttccg
     1981 ggaacccggt gcaagacccg gaccagggcc gactcttcct tacgattttt caaagtgatt
     2041 tgccaactct ccttgcgcac ccccagattg ttctgtttat tcaaacaggt tctctcacat
     2101 ttcagatcga aagcctcgcc gatacagagc ttgatcgcct catccttcgg ggtatgcccg
     2161 atctcgtctt caccgatgaa ggttaaaccg tgatccgcct catcccgctg gtaaaacttg
     2221 aacttcccgg cagggaacgg aacccctaag ccggattggt tctgattcgt aaactccaca
     2281 ataatccggg catcctcgga atacggcgca atctcataat aacggcggta tggcaccttc
     2341 tcggcatcaa aaagacgaat ctgtttggtc tggttatcct tgagagacgt taccccctgc
     2401 aatggataga gatggtagtc ggaaaaaccc ttctcctcaa agtccggtgc ggttttctcc
     2461 tcggccggac tcatcggtaa acaatagtcc gccctcagca tctcccggac ccggttaacc
     2521 tcgcccgcca aaagtttcaa ccgggccccg tgaaaggtaa tcccggattc attgcgcaat
     2581 tcaacccagg cgtcaagccc caggtgatcg ccttgcagct cggcgacata atgtgtggac
     2641 caagagagtc cattggtaat ataggaaatg cgcatcccgg ttgcgtgaga cggccggagc
     2701 cggaagatta gcgccgggcg ggccgaaagc ccctggggca gcttcggcag gaccaattca
     2761 cccgcgggct ccaccagaac ttcgccggtt tggatgtctt ccaggataag ttctacgggg
     2821 ttcaacagcc ggcacgcccg tttattgttg gtctccacct caacgagcag cactgtctcc
     2881 ccgagatatt tgccgagtaa tctttgctta tcgaccaggt cgtattcgta gttcacttca
     2941 accacatcga tgccctcgac ccgtaaggaa tcgacctcga tacgcttcgc cacgtc
//
LOCUS       DASVMB010000062         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_6239,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000062 DASVMB010000000
VERSION     DASVMB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6239"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VF531_02030"
     CDS             <1..671
                     /locus_tag="VF531_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712776.1"
                     /translation="TFLQYLKVWDSIPLLRFCLNTVIFAGSATLLSVLFDSMAGYAFA
                     RINFKGKNILFALVMITMMVPFQVIMIPLFIEVFKLGLLNNYLGLILPRATSAFGIFM
                     MRSFFVSLPKELEEAARIDGLNEYRIYGSIMLPLCKPALLTLIVFHFMNNWNDLLYPL
                     MLTSSTEMRTLSAGLAMFVGQRVIEYGPTLAAAVISILPLLMVYLISQKYFIQGIATT
                     GMKN"
     gene            783..>2278
                     /locus_tag="VF531_02035"
     CDS             783..>2278
                     /locus_tag="VF531_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEX7712777.1"
                     /translation="MKNAKMIISKDFKIGRTDDMLFGSFVEHMGTAVYNGIYEPGHSE
                     ADPNGFRKDVMELAKELQLKAIRYPGGNFSSGYNWEDTIGPKEKRPVKLELAWRAMEP
                     NSFGLNEFLTWSGMLGVAPIMTVNLGTRGIDAARNIVEYCNFPQGSYWSDLRKSHGYQ
                     EPHRVKFWCLGNELDGAWQIGYKTADEYGRLACKASSVMKLVDPDIKLIAVGSSSRTM
                     ATFPEWDQTVLTHAYEQVDYLSLHNYIGKKSDDTATYLARALDMDKQIRKIVATCDYV
                     KSKLRSNKTMYLSFDEWSVVRKADVQYTPWQIGSPFDWVRFTMEDVLAFGSMLLTLLR
                     NADRVKIACQSLLVNTNSLILTEKGGKAWRNSTFYPLLHASRYGRGEVLINVLDSPKY
                     DTTVYSDVPVVDSVAVFSEERNEVTVFAINRGNEQVMLEMDIRDFPGCSIKEHIILQH
                     EDLNAVNTAENPFEIVPAVCNNTVIHEGRVESILAKYSWNVIRFDLNKA"
BASE COUNT          607 a          451 c          563 g          657 t
ORIGIN      
        1 ttacgttttt acagtatctt aaagtatggg acagcattcc attgctccgt ttctgcctca
       61 ataccgtcat tttcgccggg agcgcgacct tgctttccgt tttgtttgat tcaatggccg
      121 ggtacgcctt tgcacgaatc aattttaagg ggaaaaatat tttatttgcc ctggtcatga
      181 tcacgatgat ggtgcctttt caagtgatta tgatcccctt gtttattgag gtttttaagt
      241 tggggctgct aaacaactat ctgggactta ttctgcccag agcaaccagc gcgttcggca
      301 tattcatgat gcgttccttt tttgtttcat tacccaagga actggaagaa gcggcgcgga
      361 ttgacggact aaacgaatac cggatttacg gctcaatcat gctgccgctc tgtaaaccgg
      421 ctttattgac tctgattgtt tttcacttca tgaataattg gaacgatctg ctttatccgc
      481 tcatgctcac cagttcgacg gagatgagaa cgctttcggc gggtttggca atgtttgtag
      541 ggcaaagagt gattgaatac ggaccaactc ttgccgccgc cgtgatttcg attcttcctc
      601 ttttgatggt ctatcttatt tcccaaaaat atttcatcca gggtatcgca actacgggga
      661 tgaagaacta gagttatttg ttctttgtgg atgaaatact gtattttaca ggaatccgga
      721 cgattcatac attctatttg ttttggagat tcctgctaat ttatgaatat ggaggctatg
      781 caatgaagaa tgcaaaaatg attatcagta aagattttaa aataggcaga accgatgata
      841 tgctgttcgg atcttttgtg gagcatatgg gaactgccgt ctacaacgga atttatgaac
      901 cggggcattc ggaagcggat ccgaacggat tccgaaaaga tgttatggaa ttggcaaaag
      961 aattacaatt gaaggctatc cggtatccgg gcggtaattt ttcgtccggc tacaattggg
     1021 aagacaccat tggcccaaag gaaaagcggc cggtgaagct tgagttggcc tggcgcgcta
     1081 tggagccgaa tagttttgga ttgaatgagt ttctgacttg gtccggcatg ttgggcgtcg
     1141 ctccgatcat gacggtcaac ctcggcaccc ggggcattga tgctgcgcga aacattgtcg
     1201 aatattgcaa ttttccccaa ggaagctatt ggagcgatct aaggaaatct catggatatc
     1261 aagagcctca ccgggtcaag ttttggtgtc tcggtaacga gctggacggg gcgtggcaaa
     1321 taggctataa aacagccgat gagtatggcc gtttagcctg taaagcgagc agtgttatga
     1381 aactggttga cccggatatc aagctgatcg ccgtcggtag ttcttccagg accatggcga
     1441 catttccgga atgggatcag accgttttga cgcatgctta cgaacaggtt gattatcttt
     1501 cgttacataa ttatatcggt aagaaaagtg acgacacagc cacatatctg gcaagggccc
     1561 tggatatgga taaacaaatc aggaaaatcg ttgcaacctg tgattatgta aaatcaaagc
     1621 tgcgaagcaa caaaaccatg tatctctcct ttgatgaatg gagtgtagtc cgaaaagccg
     1681 atgtgcagta tacaccttgg caaataggca gtccatttga ctgggttcgg tttaccatgg
     1741 aggatgtcct ggcttttggt tcgatgctat tgacattgct ccgtaatgcc gatcgggtaa
     1801 aaatcgcctg tcagtccctg ctggtaaata caaattcttt gattctgacg gaaaagggcg
     1861 gaaaagcctg gcggaactct accttttatc cattattgca tgcttcccgg tatggcaggg
     1921 gagaagtgct catcaatgtt ttggattccc caaaatatga tacaactgtc tactctgatg
     1981 tacctgtcgt tgattcagtg gccgtattta gtgaagaaag aaatgaagtg actgtatttg
     2041 caatcaacag aggcaatgag caggtgatgc tggaaatgga tatcagagat tttccgggtt
     2101 gtagtatcaa agaacatatt atactgcaac atgaagatct caatgctgta aacacggcgg
     2161 aaaatccttt tgaaattgtg ccggctgttt gcaataatac ggtaatccat gaaggccgcg
     2221 tcgagagtat actggcgaaa tattcatgga atgttataag gtttgatctc aacaaagc
//
LOCUS       DASVMB010000063         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_228201,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000063 DASVMB010000000
VERSION     DASVMB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228201"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1205)
                     /locus_tag="VF531_02040"
     CDS             complement(117..1205)
                     /locus_tag="VF531_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX7712778.1"
                     /translation="MKITFPYMGSPLIYRKLFELLGQEVIMPPRPTQRTVNLGVKYSP
                     EFACFPYKVMLGSYIEAIENGADTIVTSGGNGPCRAGFYSVVHLKTLQSMGYNVEMIV
                     FDDFYRDPGLFLKKVYKLKGENSWLALFKAVLTTYHLAHTLDSLQKIVEIQRTYEMRR
                     GSFTQAFDIICHRFSGEAKTVRDIDRIKSKAIAELEAIPVRKPPEGQRIRIGIVGEIF
                     VVMESSINLEIAELLNHLGCEVTRSMYISEWVDHALPRIFGKSATAILKNSDPYVEIG
                     IGGHERHNIGNMVEYKKQGFDGIIHLMPFACLPELVTQSMIPKISGDLDIPILTLAID
                     EQTGVANILTRLEAFIELVKNNKLARTA"
     gene            complement(1208..2191)
                     /locus_tag="VF531_02045"
     CDS             complement(1208..2191)
                     /locus_tag="VF531_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX7712779.1"
                     /translation="MRVGLPSALANPFYISFWKTYFSELGVTVEETGATTKAILDKGV
                     RHSVPEICIPVKAYTGHVVELLDRGADLIYIPRFVSIRKGDTFCPKFLGLPDMLKCTV
                     PGLESRLLTHHLQSNTEDIATETNYLNIGEIFTTDTGKIKTAIRKGRTKWLEFRKLCY
                     SKNYNCQTANEYLLDGKTATSKQSPVKIGVIGYVYNIYDSIIGMDILTRLNDLGVQPI
                     TFEMLDSMTLEKQLRRFPKNLFWAFSNKLLAAAYSFFEDPEIAGVIHVTAFGCGPDSF
                     LGKVLALDSAKYNKPFLTVRVDEHTGESHLQTRIEAFTDMILKNGYPTECA"
     gene            complement(2324..3028)
                     /locus_tag="VF531_02050"
     CDS             complement(2324..3028)
                     /locus_tag="VF531_02050"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712780.1"
                     /translation="MIKVERLEKSFGPLRVLDGLSLEFPEGKITALLGPSGCGKTTLL
                     NIIAGLGHPDAGAVHCPEMISYLFQEPRLIPWLTVRGNIALVLEDKMPQEAAVREVSY
                     YLSATGLEAYAEFYPSQLSGGLRQRVAMARAFAYQAPLLLMDEPFKSLDLRTRFRMLK
                     DFIMLWQARPRTVITVTHDLKEGLWLGDQVILLTDKPARVARRVMLDIPREERLGDHR
                     VLTLENELTELILESI"
     gene            complement(3021..3791)
                     /locus_tag="VF531_02055"
     CDS             complement(3021..3791)
                     /locus_tag="VF531_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712781.1"
                     /translation="MKPSIISPKLAPLFGILSVILGWQLVATLIHAPLLLPSPGTTLA
                     ELRALAGTGDFWHHLGVTLVRGVAGFGIAFLTGLTVGIVAGRFRAAGEFLRPIIVFIR
                     STPVMSVIILALIWFRRETVPIFVIFLMAFPIVVQNVMEGIRGIDPELREMVKVFRVG
                     KYRSFTRLYLPGLAPFLAAGVSAGLGLTWKVLIAAEVLSYPKWGIGSQMDTARVFLQT
                     EKVFAWTLVVIALGLIFDYLLEFLLKLPFAKWRRSAHD"
     gene            complement(3766..>4030)
                     /locus_tag="VF531_02060"
     CDS             complement(3766..>4030)
                     /locus_tag="VF531_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712782.1"
                     /translation="ALKEFLKNLETAISWANTNPAAAGLLAEKYVQIPATIAQKGMVR
                     SNLRYADAYKVRREVDQFLLRLVEYAPDSVGGKVPDEAFYYQP"
BASE COUNT         1081 a         1045 c          912 g          992 t
ORIGIN      
        1 ggtccgaata tattgggacg ataacagctc tccttccggg ctcagagcga tgatattggt
       61 gctaaccgaa cccacatcga cacccagata tgccttaatc ataatcgcgt cctcccctat
      121 gccgttcttg ccagtttatt atttttcacc aattcaataa aggcctccaa ccgggttaat
      181 atattggcca caccggtttg ttcgtcgatt gccaaagtca ggatgggaat atccaaatcg
      241 ccggaaattt tggggatcat gctctgtgtg acgagttccg gcagacaagc aaagggcatc
      301 aaatgaataa ttccgtcaaa tccttgtttt ttgtactcaa ccatattccc gatattatgg
      361 cgctcatgcc caccgatacc aatttcaaca tatggatcac tgttttttaa gatggccgtg
      421 gctgatttgc caaaaatcct gggtagggca tgatcaaccc actcggaaat atacattgag
      481 cgggtcacct cgcagccaag gtggttcaac agttcagcaa tttccaagtt gatggaggat
      541 tccattacta caaaaatctc gccgacaatt ccgattcgaa ttcgttgccc ctctggcggt
      601 ttgcggacgg gaatcgcctc taattcggca atcgcttttg attttatacg atcaatatcc
      661 cgaaccgttt tcgcctctcc ggagaaacgg tgacagatga tgtcaaaggc ctgtgtgaat
      721 gaaccccgtc tcatctcata ggttctttga atctcgacga tcttttgcag cgagtcgagg
      781 gtgtgagcca agtgataggt agtaagaacc gccttaaaaa gtgcaagcca ggaattctcg
      841 ccttttagct tgtaaacctt cttcaaaaat aacccggggt cccggtaaaa atcgtcaaat
      901 acaatcattt caacattata acccatactt tgaagcgttt ttaaatgaac tacgctataa
      961 aatcccgcgc ggcaaggccc gttcccgccc gatgtaacaa tggtatctgc cccgttttcg
     1021 atggcttcaa tatacgaccc taacattacc ttataaggaa aacaggcaaa ctccggacta
     1081 tatttcactc ccagattgac tgtacgttgg gtgggacggg gcggcataat cacttcttgt
     1141 cccaacaatt cgaataattt tcggtaaata agcggtgagc ccatataagg aaaggtgatt
     1201 ttcattttta agcacactcc gtcggatagc cgttttttaa aatcatatca gtaaaagcct
     1261 cgattctcgt ttgcagatgg ctttcgccgg tatgttcatc cacccttacc gttaaaaagg
     1321 gcttattata ttttgcggaa tcaagtgcca ggaccttacc caaaaatgaa tccgggccac
     1381 acccaaacgc cgtcacatga attaccccgg cgatctccgg gtcttcaaaa aaactatagg
     1441 ctgcagccag caatttattt gaaaatgccc agaacaaatt cttaggaaac cgccgcaact
     1501 gtttttccag tgtcatacta tccagcatct caaaggttat gggttgaact cctaaatcat
     1561 taaggcgggt cagtatgtcc ataccaatga tactgtcata aatattataa acataaccaa
     1621 tgactccgat cttgacggga gactgtttgg atgtcgccgt tttcccatct aataaatatt
     1681 cattggcggt ttgacagtta tagtttttcg aataacacag tttgcgaaat tcaagccatt
     1741 tcgttctgcc tttccgaata gccgtcttaa tcttgccggt gtctgtcgta aagatctcac
     1801 caatattcaa ataattggtt tcagtcgcta tgtcttcggt gtttgattgt agatggtgag
     1861 tgagtaacct gctttccaaa ccgggaaccg tgcatttcag catatcgggg agtcccaaaa
     1921 atttggggca aaaagtatca cctttacgaa tcgaaacaaa cctgggaata tagatcaaat
     1981 cagccccgcg atccaacagt tcgactacat gtccggtata ggccttaacc ggaatgcaga
     2041 tctcaggaac ggagtgccgg actcctttat ccaggatagc tttagtggta gctccggttt
     2101 cttcaactgt cacccccaat tcggaaaaat aagtcttcca aaagctgatg taaaaggggt
     2161 ttgctaaggc ggacggtaat cccactcgca tttcaatccc tccatgaact ggcttttctt
     2221 gacagagtat tcgctgcaag tgtctcgata agaataataa gtgagtactc actcattata
     2281 acagctaaaa ccaccttttg caagaaaccg ataaggactc cgatcatatc gattctaaaa
     2341 tcaactctgt caactcgttt tccagggtta gtacccgatg atccccaagc cgctcttctc
     2401 tgggtatatc cagcattacc cttctcgcta cccgtgcggg cttatcggta aggaggataa
     2461 cctgatctcc cagccaaagc ccttccttga gatcatgggt tacggtgatc accgtacgcg
     2521 gccgggcctg ccacaacatg ataaaatcct tgagcatccg gaaccgggtc cgaagatcca
     2581 acgacttgaa aggttcatcc attaaaagta aaggcgcctg gtaggcaaaa gcacgggcca
     2641 tggcaacgcg ttgcctgaga cctccactca actgagaggg gtaaaactca gcgtaagcct
     2701 caagtccggt ggcggaaaga taataggaaa cctcgcggac ggcagcctcc tgaggcattt
     2761 tgtcctcaag taccagagca atattccccc gtaccgtcag ccatggaatc agccggggct
     2821 cctgaaaaag ataactgatc atctcggggc agtggaccgc acctgcgtcg gggtgaccaa
     2881 gtccggcgat gatgttgagc agggtggtct tcccgcaccc ggatggacct aaaagtgccg
     2941 tgatctttcc ttcggggaac tcaagcgaga gtccatccag tacccgcaaa gggccgaagc
     3001 ttttttcaag tcgttccacc ttaatcatgg gccgacctcc gccatttggc aaagggtaat
     3061 tttaataaaa actccagtag ataatcgaag atgagtccca gggcgatcac caccaacgtc
     3121 caggcgaaga ccttctcggt ctgaagaaag acccgggccg tatccatctg ggaaccgatg
     3181 ccccacttgg gatatgatag aacttcggcg gcgatgagta ccttccaggt cagacccaga
     3241 ccggcgctga cccccgccgc gagaaacgga gccagtcccg gcaggtataa ccgggtgaag
     3301 ctccggtact ttccgaccct aaacaccttg accatctccc ggagttccgg atcgataccg
     3361 cgaattccct ccatgacatt ttgaacaacg atgggaaagg ccattaaaaa aatgacaaag
     3421 atcggtaccg tttcgcggcg gaaccagatc aatgccagaa tgatcactga catcaccggg
     3481 gtactccgga taaagacgat gattggccgt agaaactccc cggccgccct gaagcggccg
     3541 gcgacgatac cgactgtcag tcccgtgagg aaggcgatac cgaaaccggc cactcccctg
     3601 acaagagtga ctcccagatg atgccagaaa tcccctgtcc cggccagagc tcggagttcg
     3661 gccagggtcg ttccggggga aggtaacagt aacggcgcgt gaataagggt ggcaaccaat
     3721 tgccacccta aaataactga aaggattccg aatagcggtg ccaatttagg gctgataata
     3781 gaaggcttca tccggcacct ttcccccgac cgaatccgga gcgtattcca ccagccgtag
     3841 caagaattga tcgacttccc gccggacctt atatgcatcg gcataccgta aattgctgcg
     3901 gaccattccc ttttgcgcga tggtcgccgg tatctgcaca tatttttctg ccagcaatcc
     3961 ggcagctgcc ggattggtat tggcccagga tatggcggtt tccaaattct tcagaaattc
     4021 ctttaacgct
//
LOCUS       DASVMB010000064         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_523255,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000064 DASVMB010000000
VERSION     DASVMB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523255"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..727)
                     /gene="lptB"
                     /locus_tag="VF531_02065"
     CDS             complement(8..727)
                     /gene="lptB"
                     /locus_tag="VF531_02065"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7712783.1"
                     /translation="MSIQVQGLIKEYQKRRVVNGVDLLVNPGEVVGLLGPNGAGKTTT
                     FYMIVGLERCNAGSIYIDDHDVTALPMHIRSKYGVSYLAQEASIFRKLTVADNIMAIL
                     ELTDIPPEYRKNRCEQLMEEFHLTHLRKSMGYMLSGGERRRVEIARALAMDPRFILLD
                     EPFTGIDPIAVGDIQEIISKLREKGMGILITDHSVRETLAITDRAYIMYSGQILVSGS
                     SAEIAQDENARKLYLGDHFAM"
     gene            complement(766..1479)
                     /locus_tag="VF531_02070"
     CDS             complement(766..1479)
                     /locus_tag="VF531_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX7712784.1"
                     /translation="MRSLQEMDPDCRKGPNYKGCKLRTIHRYRFQLIVITLLLLAGSL
                     TAWASEQPRDAEQPRLQAPGGIEMDFLKNIAKCYGSDAKPAEASWTDTILTANYFEYD
                     RSRQLVRAKGHVRVVQKQDNDSLISESASLEWDRLDDRLELTGNPHFTFDGWKIDCAR
                     LEGRVNKGLFTFYGPVQGNNHETSLQAGEMILDRGASKLYLRDNPVVTQGKNRFTAAV
                     IVYDLKTKKAYSESETTLN"
     gene            complement(1516..2100)
                     /gene="lptC"
                     /locus_tag="VF531_02075"
     CDS             complement(1516..2100)
                     /gene="lptC"
                     /locus_tag="VF531_02075"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7712785.1"
                     /translation="MSRKKWLILILGFILLSVIVIIGLIFFHPVATSPKLPAVKGELK
                     LEADEMEGLSIKVAGTNHSGFWELNFAEFVNRDTEGKFQTVQGRYFQGKRQLYQIKAE
                     SGTVSWQERRVRFIGKVHLETEDGKELDADEVICTPEDGKLSARGGVSFRTPDLIYET
                     SRLDSDMNLRQIRSRGSTQVRFLKKRQYSTELHP"
     gene            complement(2313..>2692)
                     /locus_tag="VF531_02080"
     CDS             complement(2313..>2692)
                     /locus_tag="VF531_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HEX7712786.1"
                     /translation="KDVRLGFRVAKNMGGLDIGQSVVVKQGVVLAVEAIEGTDQAIRR
                     GGSLGGPGSVVVKVSKPQQDERFDVPTVGKSTVESMIAAGSGVLGIEARKTLVTEQKD
                     LMELAVRHQLKIVAITADELKLD"
BASE COUNT          692 a          727 c          556 g          717 t
ORIGIN      
        1 ctccccgcta catcgcaaaa tggtcgccca aatagagttt tcgggcgttt tcatcttgcg
       61 ctatctctgc ggaactgccg gaaaccagta tctgccccga atacatgata tatgcccggt
      121 ccgtaatggc caaggtctcc cggacgctat gatcggtaat gagaataccc atcccttttt
      181 cccgcagttt actaatgatc tcctgaatat ctcccacagc gatggggtca atcccggtga
      241 acggctcatc cagtaaaata aagcgcggat ccatggctaa agctctggca atctcaaccc
      301 ggcgccgctc accaccggag agcatataac ccatgctttt ccgaagatga gtcagatgga
      361 actcttccat taactgttca caacggttct ttcggtactc gggaggaata tcggtcaatt
      421 ccagaatcgc catgatatta tcggctacag tcagcttacg gaagattgag gcctcctggg
      481 ccaaataact aacgccgtat ttggaccgaa tatgcattgg caatgccgtg acatcatgat
      541 cgtcgatgta aatacttccg gcgttgcaac gttccaaacc cacaatcata taaaaagtgg
      601 tggttttacc ggcgccgttc ggtcccaaaa gcccgaccac ttctccggga ttgaccaata
      661 gatcgactcc gttaacgacc cgcctttttt gatattcttt gatcagccct tgtacctgga
      721 tggacatatt gatctcccat ttaaatatta tggcgtcctt gaattctaat tgagtgtagt
      781 ctcggattcg ctataagcct tcttcgtttt tagatcgtat acgatcacag cagccgtaaa
      841 tcggttctta ccctgcgtta ctacgggatt atctcgtaaa tacaatttgg aggcgccccg
      901 gtctaagatc atctctccgg cctgtaacga ggtttcatgg ttattcccct gaaccggtcc
      961 gtaaaaagtg aataatccct tattgacccg gccttcaagc cgtgcgcagt caattttcca
     1021 cccatcaaag gtaaaatgag ggttacccgt cagctcaagg cgatcatcca accgatccca
     1081 ctcaagcgag gcactctcac taatcaacga atcgttatcc tgtttttgaa cgacccggac
     1141 atgacctttc gcccgaacca actgccggga acgatcatat tcaaaataat tggcggtcag
     1201 gatcgtatcc gtccagctgg cttcggcggg tttcgcatca ctcccgtaac atttcgcgat
     1261 atttttcaga aaatccattt caatcccgcc gggtgcttgc aggcgaggct gttcggcatc
     1321 acgaggttgc tcggaagccc aagccgttaa ggatccggct aacagtaaca gcgtgatcac
     1381 gatcaactga aaacgatacc ggtgtatcgt tcttagctta catcctttat aattcggtcc
     1441 ttttcggcag tccgggtcca tttcttgaag agacctcatc tttcacctcc ggggttccgg
     1501 gaatctatcc gaagcttacg ggtgtaactc tgtcgaatat tgtcgttttt ttaaaaaacg
     1561 gacttgtgtc gaaccgcgtg aacggatctg ccttaagttc atatccgaat ccaaccggga
     1621 agtttcgtaa attagatcgg gggttctaaa actcactccg ccccgggcgg agagttttcc
     1681 gtcctcgggt gtacaaatca cctcatcggc gtcaagttcc ttgccgtctt cggtttccaa
     1741 atgaaccttg ccaatgaaac gcacccgtcg ttcttgccag gaaacggttc ctgattccgc
     1801 cttaatctgg taaagctgtc gtttaccttg aaagtaacgg ccttgaacag tttgaaattt
     1861 gccttcagta tcgcggttaa caaattcagc aaagttcaac tcccaaaagc cggaatgatt
     1921 ggtgccggca actttgatag ataaaccctc catctcatcg gcctcaagct tcaattctcc
     1981 cttgacagca ggtaacttgg gactggtcgc aacggggtga aaaaaaatca acccgattat
     2041 gacaataacg gataataaga taaagcctaa aatgagtatc aaccattttt tacgtgacat
     2101 ccgtacctca cgcggtaatt cgctacataa attatgcata cggctagcat aatttggagt
     2161 gacgaattac ttaaataaag catctcttga tgattccaaa acgattcatt tcatgacggt
     2221 ctactaggat attcgttaac taaatataaa atcctgccga tacccttccc ggatcagttg
     2281 acaaaaccgt caacatttac taaattgtaa attcagtcca atttcaattc atcggcggta
     2341 atcgctacga tcttcaactg gtgccgaacg gccagttcca tcaaatcctt ttgttcggta
     2401 accagtgttt tacgggcttc aatccctaac actcctgagc cggcggcgat catcgattcg
     2461 accgtcgatt taccgacggt aggcacgtca aaacgctcat cctgttgggg tttacttacc
     2521 ttgaccacca ccgaacccgg gcctcccaat gaaccacccc gccgaatcgc ctgatctgtg
     2581 ccttcaatcg cctcaacagc caggaccaca ccttgtttga cgacgacact ttgaccgata
     2641 tccagacctc ccatgttttt ggccacacga aaacccaatc ttacgtcctt aa
//
LOCUS       DASVMB010000065         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_374445,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000065 DASVMB010000000
VERSION     DASVMB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374445"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..466
                     /locus_tag="VF531_02085"
     CDS             89..466
                     /locus_tag="VF531_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712787.1"
                     /translation="MANFIPLIVKVVLAALLAVWLYRDSRGRDYSWILWTLVPFMVIF
                     VNSLAFAIVFAILIVVFYLIFRPKGAMIRCPKCNKPIHEILTICPFCRKDAKRECLNC
                     HEPVPWDAEQCPFCKSRALTKGN"
     gene            572..1504
                     /locus_tag="VF531_02090"
     CDS             572..1504
                     /locus_tag="VF531_02090"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7712788.1"
                     /translation="MGLQPQRIDNLIDYLDRMESILDESDFTPDEREKLIETLRLEVV
                     EVLKVAFNEGDRTIRRSAIYGLSKIGLNQSNDFFLSALSDSDEHVRGWAAEALGVLRD
                     SRTIPALIKALADENHYVCFNVAAALKQFAPDQLLHHLVGALKDNDRYIRRRVGKLLG
                     EIGDLTVTDALISALHDEDAGVRYEAAEALRKLKSSAAILPLISALTDSNHDVRAVAI
                     MALGNFGDERAIEPLTAMLVEQKDHRHACLESLSQICGEENMENFYELIRNSHKCINE
                     TSRTVLEHIYAKRAGKSTENPASCYQSVGNHYNY"
     gene            1664..2437
                     /gene="surE"
                     /locus_tag="VF531_02095"
     CDS             1664..2437
                     /gene="surE"
                     /locus_tag="VF531_02095"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX7712789.1"
                     /translation="METTKLRILLTNDDGFNAEGLQTLYSELKMKADVTIAAPDRERS
                     AVSHGITMYHPLRAIPVTIETASHWMIDGTPADCVKLAVERLFSVPPDLVISGINRGP
                     NLGNDVLYSGTVSAAIEGAINHIPSIAVSLAEFGKADFKPAAQFVTQKLNQLAELARH
                     AVLNLNFPMRCRGVKLTRLGERRYENVFDERVDPRGQTYFWMGGQPVQSVQFQDTDIQ
                     AVDDGYISITPLHFDLTDHQFLHDQNLCDLFASGISYPE"
     gene            2614..3018
                     /locus_tag="VF531_02100"
     CDS             2614..3018
                     /locus_tag="VF531_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712790.1"
                     /translation="MVQIERDDLVRALKSFKGLAKQDLLASGPSRQAEYRKTHAVARR
                     EIYAKLIDLVESSGIQNACVFAFQEYRNLMAGGDLPTADPISSGHFQALEMFFNVLGV
                     GTDKLRSCVDESSDLDQLLNTAPSFEDNLCMQ"
BASE COUNT          822 a          642 c          731 g          851 t
ORIGIN      
        1 tccgacattt tcccagagtt tgtttttttt aaaatcggca ggaatcagga attcaaaggt
       61 gaaataataa tagaaaaggg ggattcttat ggctaatttt attccattga ttgtcaaagt
      121 ggtccttgcg gctttacttg ccgtatggct ctatcgcgat tcccgcggcc gtgattattc
      181 ctggatttta tggactttag tgccgtttat ggtcattttc gttaattcat tagcttttgc
      241 gattgttttt gccatactga tcgtggtctt ttatctgatt ttccgtccga aaggcgcgat
      301 gattcgttgc ccgaaatgca ataagcctat tcatgagatc ctgacgatct gccctttctg
      361 tcgtaaggat gccaaacgtg aatgcttgaa ttgccatgaa ccggtaccct gggatgcgga
      421 acaatgcccc ttctgcaaat caagggcttt aaccaaggga aactaaaggc gttgtgacat
      481 ccggtcattc ccccaatctc cggctttttt ggggtgggga aatctttaag gtaaacgggt
      541 tatggtttgg aataaagagg gggcagccaa tttgggttta cagcctcaac gtatcgataa
      601 tttaattgat tatcttgaca ggatggaatc gattctggat gaatcggatt tcacaccgga
      661 tgagcgggaa aaactgattg agacattaag gttggaagtt gttgaagttc tgaaggtggc
      721 cttcaacgag ggagatcgaa cgattcgacg cagtgcaatt tacggtttga gtaaaattgg
      781 tcttaatcaa tcgaacgact tcttcttgag cgccctgagc gattcggatg agcatgttcg
      841 gggttgggca gccgaggctt taggtgtatt gcgggattcc aggacgattc ccgcattaat
      901 taaagcattg gctgatgaaa atcactatgt ttgttttaat gtagctgccg ccttgaaaca
      961 atttgccccg gatcaacttt tgcaccatct agtcggtgca ctcaaagata atgaccggta
     1021 tattcggcga cgggttggca aactccttgg tgaaattggt gatctaacgg ttaccgatgc
     1081 cttgatatcc gctcttcatg atgaggacgc cggagtacgt tatgaagctg ccgaggcatt
     1141 gcgaaaattg aagagttccg cggcgattct tccgttaatt tcagccttaa ccgattcaaa
     1201 tcatgatgta cgtgcggtag cgattatggc tcttggaaat tttggtgatg agcgagcgat
     1261 tgaacccttg accgcgatgc tggtcgaaca gaaagatcac cgccatgcat gcctggaatc
     1321 tttatctcaa atttgcggtg aagaaaacat ggagaatttc tatgaattaa tccggaacag
     1381 tcataaatgt atcaatgaaa cctcccgaac ggttttggaa catatttacg ccaaacgcgc
     1441 gggcaagtcc actgaaaatc ccgcctcctg ctatcagtcg gtgggcaacc attataatta
     1501 ttgatggagt tcggcaagaa ttgttccatt ttccccggtt tgccgcgttg gtaaaaacca
     1561 tcaggcaacc aaaattaccc aagtaattaa attacttaag taataaaatt acttcagtaa
     1621 ccgcatagtt tattccaaaa taagatataa ataaatatag gttatggaaa ccactaaact
     1681 tcgaatttta cttaccaatg atgacggatt caatgcggag ggtttgcaaa ccctttactc
     1741 cgagctgaaa atgaaggccg acgtaacgat tgcagcccct gatcgtgagc gaagcgccgt
     1801 cagtcatggt attaccatgt atcatccttt acgggctatt ccggtcacca tcgaaacagc
     1861 cagtcactgg atgatcgacg gcactccggc agattgcgtg aaacttgcag tggagcggct
     1921 ttttagtgta ccgccggatt tagtaatctc cggcatcaac cgcggtccga acctgggcaa
     1981 cgacgtcttg tattccggaa cggtttccgc cgcaattgaa ggagcgatta atcatattcc
     2041 aagcattgcg gtttcattag ccgaattcgg taaggcggat ttcaaacctg cagctcaatt
     2101 tgtcacacaa aaattgaatc agttggccga gttagcccgc cacgccgtat tgaatttgaa
     2161 ctttcccatg cgatgtaggg gagtcaaact cacccggtta ggagaacgcc gctatgaaaa
     2221 tgtatttgac gagcgcgttg atccccgtgg tcaaacatat ttttggatgg gtggacagcc
     2281 ggtacaatcg gttcaattcc aggataccga tattcaagcc gtggatgatg ggtatatttc
     2341 gatcacacca ctacattttg atcttacgga tcatcagttc ttacacgatc agaatctttg
     2401 cgaccttttc gcttccggca tcagttatcc cgaataatga tttctgtctt tcgaagggat
     2461 aggagtatgg ggaaaaccca aacaacgatg ataaaacagg acatttggat acaattggaa
     2521 agcaggattt catctccgat tagcgaaatt aatagtaagc agtatccgat tactgccggg
     2581 gagataaaaa aagaataaag gaagggatgc cccatggttc agattgaacg ggatgatctg
     2641 gtgcgggcgc tcaagagctt caaaggttta gccaaacagg atctgttggc tagcggacca
     2701 agccgacaag cagagtatcg aaaaacacac gcggtggcac gtcgcgagat ttacgctaaa
     2761 ctcattgatt tagtggaaag ctcgggaatc caaaatgcgt gtgtttttgc ttttcaggag
     2821 tatcgcaatt taatggcggg cggtgatctt ccaaccgcag atcccatatc aagcggccat
     2881 ttccaagcct tggaaatgtt ctttaacgtc cttggagttg gcaccgataa actacggtcc
     2941 tgtgtggacg agagttccga tctggatcaa cttttgaata cggccccatc ttttgaagac
     3001 aacctctgta tgcaataaat ttttacactc aagttgcttg ttttcg
//
LOCUS       DASVMB010000066         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_154793,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000066 DASVMB010000000
VERSION     DASVMB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154793"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1025
                     /gene="ftsE"
                     /locus_tag="VF531_02105"
     CDS             345..1025
                     /gene="ftsE"
                     /locus_tag="VF531_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX7712791.1"
                     /translation="MIQFHEVSKVYGNGTVALNGVDLHIEKGEFVFLVGPSGAGKTTL
                     IKLAIRRELPSQGLIMVGRKNLSQMKMHEVSIFRRNIGVIFQDYKLLPNKTVFENVAF
                     ALEVIEASRKDILKQVPAVLELVGLKDKAKVFPSQLSGGEQQRVSLARAIVNRPLLLL
                     ADEPTGNLDPETSWGITDLLLEINKRGTTVVMATHNKSIVDRMRRRVVAMENGQIVRD
                     EQKGVYDT"
     gene            1022..1891
                     /gene="ftsX"
                     /locus_tag="VF531_02110"
     CDS             1022..1891
                     /gene="ftsX"
                     /locus_tag="VF531_02110"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX7712792.1"
                     /translation="MKVKTIWYFTKEASSGLSRNGLMSLAAVTTITLSLIILGCFYII
                     VSNFTHFADMAKGVLELRVYLEEGADPARLQSEMNDLDGVKSVQFVSKEEGAMWLEKQ
                     LGVKNLFVTADNPLPNMLDIKLKDDAKIKTLVPQVNSLEGVDEVVYGKTFVEAMLMVV
                     QIIWVFGLSLVVIIGLVVLYIITNTIRLTVLARWREIEIMKLVGATDWFIRWPFMLEG
                     IILGAGGALVSILLLSKGYHILFAYIKRFAPFIPLLGEGAVNYGLLWILAVVGMAFGA
                     IGSMMSIKKFLRV"
BASE COUNT          472 a          396 c          531 g          522 t
ORIGIN      
        1 aactattaat tcgtcaaaac aatcgccaga ctttataggt ttctcattgt tttattcagt
       61 gcgaacctga aaattcctgc acaaaaagtg gaggccaaat tattttgtga cagtcgtgaa
      121 gtttcaataa tcaatcatga aaaagctgcc gaaaaatatt aaagagtgcc cgggtcgatc
      181 ggtagatgac ttaacgggtg gtccgagttt gagtgaaata acgggtttac tattgatcca
      241 ggtaagatag aggtaagata ttggatcttt aaataaggaa ggatatcctg gtactgtgtc
      301 gaaataactc gcaacatcct gattttgaac aggggtgaac cgtattgatt caatttcatg
      361 aggtctctaa ggtttatggt aacggtaccg tcgccctgaa cggggtcgat ttgcatattg
      421 aaaagggtga attcgttttc ttagtgggac caagcggcgc aggaaagacg acccttatca
      481 agttggcaat tcgacgggag ttacccagtc aagggttgat catggttggc cgaaaaaatc
      541 tttcccagat gaagatgcat gaggtctcaa tattccgccg caacatcggg gtaatttttc
      601 aggactataa gttgttgccc aacaagaccg tctttgagaa cgtggcgttt gccctggaag
      661 tgatcgaagc atcccggaag gatattttga aacaggttcc tgctgttttg gaattggtgg
      721 ggctgaagga taaagccaag gtctttccgt cccaactctc cggcggcgag cagcaacggg
      781 tttccttggc gcgagccatt gtgaaccgcc cgcttttatt gctggccgat gagcccaccg
      841 gaaacctgga cccggaaacc tcctggggga ttactgactt gctgcttgag atcaataagc
      901 gtggcaccac ggtggttatg gcaacacata acaagtcgat tgtggatcgg atgcgccgcc
      961 gggtggtggc gatggagaac ggtcagatcg tccgggatga acaaaagggg gtttacgaca
     1021 cttgaaagtc aagacgatat ggtatttcac caaagaggct tcctccggcc tcagccgtaa
     1081 cggattaatg agtctggctg cggtaactac gatcacattg tccctgatta ttttgggctg
     1141 tttttatatc attgtatcca attttacgca ttttgcggat atggccaagg gtgtcttgga
     1201 actccgggtg tatcttgagg aaggggctga tcccgcccgg ttacagtcgg agatgaacga
     1261 tttggatgga gtgaaatccg tccaatttgt ctccaaggaa gagggcgcca tgtggctgga
     1321 gaaacaattg ggagtcaaga atctctttgt cacggccgat aacccgcttc caaacatgct
     1381 ggatatcaag ttgaaggatg acgccaagat caaaacgctg gtcccccagg tgaattcctt
     1441 ggagggcgtt gatgaggttg tatacggcaa aacttttgtt gaggccatgt tgatggtggt
     1501 acagatcatc tgggtgttcg gcttgagttt ggtggtcatc atcggactcg tggttttgta
     1561 tatcatcacc aacacgattc gtcttacggt gttggcacgg tggcgcgaga tcgagatcat
     1621 gaaactggtt ggcgccaccg attggtttat ccgctggccg tttatgctgg aaggaatcat
     1681 tctcggcgcc ggcggggcat tggtctcgat tttactgctt tctaaaggct atcatatttt
     1741 attcgcctat atcaaacggt tcgccccttt catcccgtta ctcggtgaag gtgcggtcaa
     1801 ttacgggctt ctctggattc tagctgttgt gggaatggcc ttcggagcca tcgggagtat
     1861 gatgtcgatc aaaaagtttt tacgggtgta aaatgctggg atgggtgacg ccgcctccgg
     1921 g
//
LOCUS       DASVMB010000067         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_596352,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000067 DASVMB010000000
VERSION     DASVMB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596352"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="VF531_02115"
     CDS             <1..2057
                     /locus_tag="VF531_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HEX7712793.1"
                     /translation="GVVITDSEWRIRRFLEKPGWGEVFSDTVNTGIYILDPKVFKYFK
                     KGQVFDFSKDLFPLMLGNGEPLFGYVASGYWSDIGNLDQYRQAHNDILTGKVRAVLPG
                     HEYRPGIWVGKNPSIDRRAELEAPLILGDDCRVEAGARIGAFTVIGKNCVIQENASTK
                     RSILWDHCYVGDNSELRGAILCHHAYLKGKNSVFEGAVLGDSVSLGSRAAVKPQVKIW
                     PSKDVESGATVNESLIWAKKSSRSLFGNSGICGGINHEITPEIAAKLGAVFGAHLKSG
                     SRMVVSCDDYRPSRIFKRALVAGALSAGVGVYDLGSQVTPLTRYALVALEAKGGVHIR
                     INPQDPEGLLLEFFDDQGLNLSKSAERSLENAYFSEDFPRVPPGEMGELTFVPQLIEP
                     YLQGLLDREIRELIKEVHFRIVAAYDRSSLAFFLPEFLKTLECRVHQIEASHSDRPRT
                     FAELLETVRTVAEEIKTSRADLGLIVDSSAERLLLLDGEGNLVKDQELVALLSYLVLK
                     YRPEATVPVQVTAPQFIEELAREFHGRVVRTKASPRSLMERVAEERLFPNGDGSTSYH
                     PQFDALFSFSKILELLARERISLREALALIPRVERGYAEVDCPWERKGLVMRNLFEEN
                     KEKQLEMTDGLKVYHDEGWALVLPDAEEPIFKIYSEAGTPEEADALTRLYMSRINELQ
                     LQ"
     gene            2312..2399
                     /locus_tag="VF531_02120"
     tRNA            2312..2399
                     /locus_tag="VF531_02120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2346..2348,aa:Ser,seq:tga)
     gene            2505..2596
                     /locus_tag="VF531_02125"
     tRNA            2505..2596
                     /locus_tag="VF531_02125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2540..2542,aa:Ser,seq:gct)
     gene            2782..2858
                     /locus_tag="VF531_02130"
     tRNA            2782..2858
                     /locus_tag="VF531_02130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2816..2818,aa:Arg,seq:acg)
     gene            3359..3481
                     /locus_tag="VF531_02135"
     CDS             3359..3481
                     /locus_tag="VF531_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712794.1"
                     /translation="MLYIAGILVLAVFAFVGYVMYCDISMRKAEKAKEEKQGKK"
BASE COUNT          879 a          873 c          896 g          929 t
ORIGIN      
        1 atggagtagt aatcaccgat tcggaatggc gaatccgccg tttcctggag aaacccggtt
       61 ggggtgaagt ctttagcgat acggtcaata ccggaatcta catcctcgac cccaaggtat
      121 tcaagtattt taaaaaggga caagtcttcg atttcagcaa ggatcttttt cccttgatgc
      181 tcggtaatgg cgaaccgtta tttggctatg tggcttccgg ttactggtcc gacatcggca
      241 atctcgatca ataccggcag gctcataacg acatcctgac cggcaaggta agggctgttc
      301 tccccggcca tgaataccgt cccggaatct gggtcggaaa aaacccctcc attgatcggc
      361 gggctgaact agaggcccct ttgattctgg gagatgattg ccgggtggag gccggggccc
      421 gtatcggagc ctttaccgtc attggtaaga actgcgttat tcaggagaac gccagcacga
      481 aacggagtat attatgggac cattgttacg tgggggataa ttccgaactc cgcggggcca
      541 tcctttgcca tcacgcttat ttgaaaggta aaaacagtgt ttttgaaggg gctgtcctgg
      601 gtgattccgt atctctgggc agccgggctg ctgtgaagcc ccaggtgaag atctggccgt
      661 ctaaagatgt cgaaagtgga gctacggtca acgagagcct gatctgggcc aaaaagagtt
      721 cccgcagcct ctttggtaac tccgggattt gcggcggcat caaccatgaa ataaccccgg
      781 agattgccgc caaacttggg gcggttttcg gcgcacatct gaaatcaggt tcccggatgg
      841 tagtcagttg cgacgactac cggccctcca ggatcttcaa acgggccctg gtggccggag
      901 ctctctccgc cggggtcggc gtctatgacc ttggatccca ggtcaccccc ttgacccgct
      961 atgccctggt cgccttggaa gccaagggtg gagtgcatat ccggatcaac ccccaggatc
     1021 ctgagggact cttactggaa ttcttcgatg atcaaggatt aaatctctca aaaagcgccg
     1081 aacgctcttt ggagaacgcc tatttcagcg aggacttccc gcgtgtcccc ccgggtgaaa
     1141 tgggcgaact cacctttgta ccgcaattga ttgaaccata ccttcaggga ttgctggacc
     1201 gggagatcag ggaattgatc aaagaagttc atttccggat tgttgccgcc tatgaccgga
     1261 gcagcctggc gttcttcctg cccgagtttc tcaagaccct ggaatgccgg gttcatcaga
     1321 tcgaggcatc ccattccgac cgtccccgga ccttcgccga gttactggag acagtccgga
     1381 ctgtggccga agagattaag accagccgtg ccgatctagg gctcattgtg gacagtagcg
     1441 ctgagcggtt gttactcctt gacggggagg gaaatctcgt taaagatcag gagctagtcg
     1501 cgctcctttc ctacctggta ttgaagtacc ggccagaggc taccgtgccg gtccaagtca
     1561 ctgcacccca gttcattgag gaattagccc gggagtttca cggccgggtc gtccggacca
     1621 aggcctcacc ccgttctctc atggaacggg tggcggaaga acgtctcttt ccgaacggcg
     1681 atggaagtac ttcgtatcac ccacagtttg acgcgctatt cagtttcagc aaaatcctgg
     1741 agttattggc cagggaaaga atcagccttc gtgaggccct ggcactcatc ccccgcgtgg
     1801 aacggggtta cgccgaggta gactgtccct gggaacggaa agggctcgtc atgcgtaatc
     1861 tctttgagga aaataaagag aagcagctgg agatgacaga cggtctcaaa gtatatcatg
     1921 atgaggggtg ggctttggtc ctgcctgatg cggaggaacc gatcttcaaa atctactccg
     1981 aggccggtac ccccgaagaa gccgacgcct taacccgcct ttacatgagc cgtatcaatg
     2041 aactacagct acagtagaag tttgaagggt gaagggtaaa aaaaaccgta tatccggtaa
     2101 atcttggggc gaagcagcct gctcaccgct gccgtgttga atatcacagc cccgcgcata
     2161 tcggaccggc gcggagttct tcttgacacc cacctgaaaa atgtgttaaa attattcatg
     2221 cattccgagt gtattgcctc cggccttaac gtctagggtt ttgcctcacg gcaccctttc
     2281 ccgaaacggc cttttggagg cggcgggcct cggaggagtg tccgagtggt ttaaggaggc
     2341 ggtcttgaaa accgttgtac cgcaaggtac cgggggttcg aatccctcct cctccgccat
     2401 aaaccaagtt caaaggttaa agggagaagg aaaaaataaa gtatggttta agtttattgt
     2461 ttattttttc actttactct ttacacttca cattttagtt caacggagag gtacccaagc
     2521 cggttgaagg ggacggtttg ctaaaccgtt agtaggagaa atcctagcga gggttcgaat
     2581 ccctccctct ccgccattgc ttattccgca acctatttag ttggttgctt ttttattgcc
     2641 ggcggcgaat ggtacattga ttcattggtt atcccgacca aagtggctta cagtttccaa
     2701 cccggatcgg acacgccatc ctttactttt cctcaccgct atgctataat tatttcttgc
     2761 ctagatcatt accaatcatt tgcgcccgta gctcagttgg atagagtgtt tgactacgaa
     2821 tcaaaaggtc acaggttcga atcctgtcgg gcgcaccagt agaaaagaca aggacttgat
     2881 gaatatcagg cccttttatt tttagcctct cataaaccct tttaaggaat tgggtgaatc
     2941 aaatggtaca ggttatatcg aaaaacaagt tactgttgat aacccttatt atcctttttg
     3001 cgttcctgtc aaacctttat ttccttagtg gagtcaacgg acaccggaat catcttgcta
     3061 ctgacctttt gtcacaggtt attgatgatc tatccaacca ggccattacc acaaaggatc
     3121 atcgctcgga ctattcttta tcctatagac ccttcaaact aaatttttca agttttaggc
     3181 tttttacatt cgactgtgaa acagtaaggc tatttattcc ccaagtctca ggcgcattaa
     3241 cgattattgg actgtttttt ctgatatact atcaattgtc ccgaacgagt agtgaaaaat
     3301 ccgcctcctg cgatgttgtt atttaaatct caataacaga caatgcagga ggtaaacaat
     3361 gctttatata gccggaatat tggtattggc agttttcgct tttgtcggtt atgttatgta
     3421 ctgtgatatt tcaatgcgca aagcggaaaa agcaaaagaa gagaagcaag gtaaaaaata
     3481 gaacgaaaaa agccccgtta tggggctttt tttaatatta tcatctatcg gattccaagg
     3541 cacaatgcta tatcaaagtt tatcataata ttctttt
//
LOCUS       DASVMB010000068         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_229916,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000068 DASVMB010000000
VERSION     DASVMB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229916"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..149)
                     /locus_tag="VF531_02140"
     CDS             complement(18..149)
                     /locus_tag="VF531_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712795.1"
                     /translation="MTNRDSPVFLRYPQYCSVKDDSDVLAANIELDDLMNRDQKLTS"
     gene            complement(193..1005)
                     /locus_tag="VF531_02145"
     CDS             complement(193..1005)
                     /locus_tag="VF531_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX7712796.1"
                     /translation="MRINNNLPLNTLNNLYTNHKHNTDAAEKLSSGRKINKAADNAAG
                     LAIAAQMTGQIGGLSQAIENSQDAVSLVQTADGALTESQSMVTRIGELSVQAANGIYT
                     DSQRGMIQSEIDQLKTGLNGIANTTSFNNQTLLDGSTKSLTFQTGPNELNNMTASIGN
                     MNSAALGVNHIDVTNAVGAGRGITAAQRATGVASAERSNLGAYQNRLDHTSKNLGVAV
                     ENQTASLSTIQDADMAAEMMDYTRTNILQQTGNAMLAQSNSNRYQVMRLLSL"
     regulatory      complement(1090..1185)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1337..1597
                     /locus_tag="VF531_02150"
     CDS             1337..1597
                     /locus_tag="VF531_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712797.1"
                     /translation="MAKSCLTQYEHSMKEGVSLAPCLGNRKVNPNWDGHLTAEQRELL
                     AKPGFGITEQLPYLPESDVKEIMNIIESCVKHVKLIIETPKS"
     gene            1972..2952
                     /locus_tag="VF531_02155"
     CDS             1972..2952
                     /locus_tag="VF531_02155"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEX7712798.1"
                     /translation="MLCILNDNTSPYFNLALEEYLLKEFADECFMLWRNEPSIIVGKN
                     QNTLAEINLEYVEAQGIKVVRRLSGGGAVFHDLGNLNFTFIVNDLENSFCDFSKFTAP
                     IIAVLAKLGIKAELSGRNDLTVSGLKFSGNAQYKYKDRLLHHGTILFSAAIPDITSAL
                     RVSSEKFEGKGIKSVASRVTNIQSHLSQPLSIEEFAVMIRNHMASDRGVYTLYSLTPP
                     DIDKVNQLVREKYATWEWNYGASPQYNLRNKARLTGGSVEVLLDVKDGTIQNARILGD
                     FFSKYDISQIERSLSGVRHEEKAVAAALASFDFQDYMANVTASEFIRLLF"
BASE COUNT          854 a          721 c          774 g          845 t
ORIGIN      
        1 cttgaatcat tgaaaattca agaggttaat ttttgatccc ggttcatcag gtcgtccagc
       61 tcgatgttgg cggccaggac gtcagagtcg tcttttaccg aacaatattg cggatatcgt
      121 aaaaagacag ggctgtctct atttgtcacg agacagcccc attcgtaccg gattcgtgat
      181 taaatccgta acctataaac tcaacaacct cattacctga tagcgattgg aattggattg
      241 ggcgagcatg gcgttaccgg tctgctgtaa aatatttgtc cgggtataat ccatcatctc
      301 ggcggccatg tctgcatcct ggatagtgga tagtgaagcg gtctggtttt caactgcaac
      361 acctaaattt ttactcgtat gatccaagcg gttttgataa gcgccgagat tggaacgttc
      421 agcggaagcc accccggttg ctctttgagc tgcggtaatt cccctgccgg cccctactgc
      481 gttcgtaacg tcgatatggt tcacgcccaa agcagcggag ttcatgttgc cgatggaagc
      541 cgtcatatta ttcaattcat tgggcccggt ttgaaaggtc aggcttttgg tcgaaccgtc
      601 cagtaaagtt tggttgttga aagaagtggt attggcaata ccgttgagtc cggtctttaa
      661 ttggtcgatc tcactttgaa tcattccccg ctgactatcg gtgtagatgc cgttggcggc
      721 ctgaaccgac agctcgccta tccgggtcac catcgattga ctttccgtca gggcgccgtc
      781 cgcggtttgg accagggaaa ccgcatcttg ggaattttcg atggcttgag ataagccgcc
      841 aatttgaccg gtcatttgtg ccgcgatagc caagcccgca gcgttgtctg cggctttatt
      901 gatcttgcgt cccgaggaga gtttctccgc cgcatcggta ttgtgtttat gattggtata
      961 taagttgttc agggtattta gcggaagatt attattaatc ctcatttttc aaataccacc
     1021 tttccacttt taagattata ttgtcaatcc gatccggaat taataacaga aacaaaaaag
     1081 caatctactt tcgggagccc ggcgatcatg gccaatctgc gtaaaattgg ctttagaccc
     1141 gtggttttgc gtcttcaact ttcgttgaat ttgccttttt cgtggtaaat tgttctatat
     1201 ttagtttagc attgaaataa tgatatgtca aaacaaggag gttacctgcg ctgatggcac
     1261 aaattgcgtc aaaaaagtat tatcatcagg gtaaggtaaa gcctgcttat aatttctaaa
     1321 ggtttatgaa ggggggatgg cgaaatcttg tttaacacaa tacgaacaca gcatgaaaga
     1381 aggggtatcg ttggcgccat gcctcggtaa tcgaaaggtg aatcccaatt gggacggcca
     1441 tttaacggcg gaacagagag agttactcgc taaaccggga tttggaatta cggaacaact
     1501 gccgtatttg ccggaaagcg atgtcaaaga aatcatgaac atcatcgaaa gttgcgtaaa
     1561 acatgtgaaa ttgatcattg agactccgaa atcataatcg agtgatatac aaacgcccac
     1621 cacgggcgtt ttattttcgc tcatcgcagg aaactactga aggcgaactc cggcagatag
     1681 attgttcaac ctaatgatat ccaccaccct aaaactcctt aaaaaataaa ggggaaaaag
     1741 gtcataaatg gaattaattt atggtgtcct taccaatcga tcactaaatt cataatgaaa
     1801 cagggggcat aggtcctcac ccctcagcca acatccgtgc ctcgcgtaca cggtacgcgc
     1861 gccctccccc ataacgctat caaaaagcga tagctatggg ggaggggccg acagtgattc
     1921 ttctgactaa actggatagc catgaataga acaatttccg gaggtttacc tatgctttgt
     1981 attctcaacg acaataccag cccttacttt aatttggctc tggaagaata cctgttgaaa
     2041 gagttcgctg acgaatgttt tatgctttgg cgcaatgaac ccagcatcat cgttgggaaa
     2101 aaccagaata ccctggccga gattaatctg gaatatgtcg aagcgcaagg gatcaaggtt
     2161 gtccggcggc tctccggggg cggggcggtc tttcatgatc tgggcaatct gaatttcacc
     2221 ttcatcgtca atgacttaga aaacagtttt tgtgatttca gcaaattcac cgccccaatt
     2281 atcgcggtcc tggcgaaact cggcatcaaa gcggaactgt cggggcgcaa cgatctgacc
     2341 gtatccggtc ttaaattctc agggaacgcg cagtataaat ataaggacag gctgctgcat
     2401 cacggtacga ttcttttttc cgcagccata cccgatatta cctcggcctt acgggtcagc
     2461 tcggagaaat ttgaggggaa ggggatcaaa tccgtagcta gccgggtgac aaacattcag
     2521 agtcaccttt cccaaccgct gtcgatagaa gaattcgcag tcatgatccg gaaccacatg
     2581 gcctccgacc gtggagttta tacgttgtat tccttgactc cgccggatat tgacaaagtc
     2641 aatcaattgg tccgggagaa atacgccacc tgggaatgga attacggcgc atcgccccaa
     2701 tataacctgc gcaataaagc ccgcctgacg ggaggctcgg tggaggtctt gctggatgtc
     2761 aaggatggaa ccatccaaaa cgcccggatt cttggagatt tcttcagcaa atatgatata
     2821 agtcagatag agcggtcttt gagcggcgtg aggcatgagg agaaggcggt cgcggcagcc
     2881 cttgcgtcat tcgactttca ggattatatg gcgaatgtta ctgcttccga gtttattcgg
     2941 ttgttatttt aactggattt tgattacggg tactggaacg gatcgatttg gcaaaataat
     3001 tccacctgtg attatccccg gtttcacgaa tgactgatca aaaaatccga caaaaacggg
     3061 gtgacccggt actacttctt ccccgagtat gaggaggagt atacgaatgg aaccaagacc
     3121 aacacgatta agtattattt tgcagccaac caacggatag ctgaaaactc gaccaaggat
     3181 ggagtccggt acta
//
LOCUS       DASVMB010000069         6187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_83267,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000069 DASVMB010000000
VERSION     DASVMB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83267"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF531_02160"
     CDS             complement(<1..489)
                     /locus_tag="VF531_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712799.1"
                     /translation="MSSRYLRRWLISLLICGMIGMGLTVTGSAATTASDGITIDEVDF
                     LVENIHPSFWAEATDPDSARLYFDFFIYVKDLKNVINQIKQVRVYDMYDSYWTLDLND
                     DIDLEEGYIGGYGRWNDGVMTENGSLLAVKNLRVEIITNNDELITKEFSIPEPGTIDS
                     FGK"
     gene            703..2358
                     /locus_tag="VF531_02165"
     CDS             703..2358
                     /locus_tag="VF531_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7712800.1"
                     /translation="MEQESKKTEERPFIFPWGSRRFGLAGLVIGFLLALGVRIPWLWE
                     IPRFIDELREVNLAYLISRGQALPLHNAAHDIGALHNYILAGIFDIFGTGIYLPRLYT
                     AVTSAMTVIFIYLSGSRLYGRVAGVVAAGLLLTNGMHILVTHMAWANCTTPFFFTFAL
                     YTTIRAAEQPSGGWLVGAGCSWALALQTHPSIIIFLPVVLFYIARTPGLQPLFSRRSG
                     VSVRYHYLAVLAFAAGYSNMLIYNLVSRGGSIRWAMQKGYTLEQSPGPVSFVHNLIQM
                     WVELIRTIGSVYQTKSGMLEYLAYPAFSAGLFMLGYGIYRAVRCRRALPVWMLLAGCT
                     VIPWINGRYGYYIATRYIMPLVICALLLIGCAANDLWKLQQRWLSRRWRPIHFAGSVL
                     ALGAIILWQQGLFYDYCRRLADTDMSNRMALQIVEQTRQMAGLDRRGLRVNRRIEILI
                     DDRVHIPNDPLPVLYRLAGLPYTVLFHSSGESDSALRIRWLGKLGSNPGIRRIMVLDE
                     QTYRKLREFIPDARVVRFETRKVLHGTHGQIRTTVFLASRVSD"
     gene            2589..2975
                     /locus_tag="VF531_02170"
     CDS             2589..2975
                     /locus_tag="VF531_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7712801.1"
                     /translation="MAKLHGIMICVTRQKTCERLIRFGYELAQKDGADLYVVHAAKVG
                     ENYLGNPNEGEALNYLFQVSKQVGAEMNVIRSKHVTKTLADFARKQKIAIMVLGAAPD
                     SASDNKIIQELQLHLPQMDFKVLPTA"
     gene            3134..3325
                     /locus_tag="VF531_02175"
     CDS             3134..3325
                     /locus_tag="VF531_02175"
                     /inference="COORDINATES: protein motif:HMM:NF025436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HEX7712802.1"
                     /translation="MDSDMMGIASASTGMKQNQILMAVQTQMLRTSMDNQAQAVQSLL
                     EALPVAGPSHLGTKIDITV"
     gene            3715..4395
                     /locus_tag="VF531_02180"
     CDS             3715..4395
                     /locus_tag="VF531_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712803.1"
                     /translation="MKKFIAVMVMTLILIGSAASIVLANPMSLRAENGLVMPASGLNL
                     DLGTGFALHNSSSTASTPSLEAQLNYGAFPGLTVAAAIEAPMNVASSNDVQKMVKLYY
                     SPIHDGRGYTLYLGYDLDKATIPVYGLSLWADSKLLLGYINLEARSENEGTAAVMITP
                     GANLHIGSKLQIGGEVEVEPENWQTGEFTLGASYAVYPKVQLKMGVITGGESFIPQSY
                     NLGLSATL"
     gene            complement(4477..5835)
                     /locus_tag="VF531_02185"
     CDS             complement(4477..5835)
                     /locus_tag="VF531_02185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7712804.1"
                     /translation="MKPRRTFSDQRRFLPWLLVVMLGLTLIPGLATAQNQTTSGTIAV
                     RDDGLTTIVNGSGAESEVQYLTLLIKTGYSQDPAAKAGLTDLTNILVYRILQMATLLD
                     FNYFLAADYSRFDFAVPNNDFTAFCRQLDAVIRIEALLSYDECNILLKNYRNLPRNPS
                     EVGLAKLYTMIDGAGHPYTKIFDNYMSNLDITDANNWFRRIYRPSNLIISSTAPLPDD
                     FLLRPSGREMKQVITKSPVPAATCSPNPLVEITTVHNSTASVFIGLPAPALTDPDLAT
                     SLLVQQYLAWELWNKLREQAGYCYDVSVFHSYLTEPVAPILLIEFETLPGNAGPAIGQ
                     VISVLQGIAKNGIPEAELTSLKQAVEKQAKRIGDSRRTATQNLALQAFFNQTWLSDYD
                     AYSKLLAGVTSADTARLIRTKLGSIKLSLTGPEETQQALVQLQPMLQKITTGLEPGKT
                     GK"
     gene            complement(5940..>6187)
                     /locus_tag="VF531_02190"
     CDS             complement(5940..>6187)
                     /locus_tag="VF531_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712805.1"
                     /translation="AAKKLDNFLSWFAKTQGRYLDSLSEDAYGLNEYYDAQLFDPAAI
                     TVRDLETIRYINSGDIRKFVNKYLAGQYSQKIVIKAL"
BASE COUNT         1550 a         1413 c         1544 g         1680 t
ORIGIN      
        1 ctttccgaaa ctatcgatgg ttcccggttc gggaatggaa aattctttgg taatcaactc
       61 gtcgttgttt gtgatgatct caacccgtaa atttttgacg gccaaaagtg agccgttttc
      121 agtcatcacg ccatcgttcc agcgtccgta accgccgatg taaccttcct ccagatcgat
      181 atcatcattg agatccagag tccagtaaga gtcatacata tcataaaccc ggacctgttt
      241 gatttgattg atgacatttt tgagatcctt gacataaata aagaaatcaa agtatagcct
      301 ggccgaatcg ggatcagtgg cttcagccca aaaggaggga tgaatattct caactaaaaa
      361 atcgacctcg tcaatcgtaa ttccatcgga agctgttgta gccgcagagc cggtgacggt
      421 aagtcccatt ccgatcattc cgcagatcaa cagggagatt aaccaacgcc ggaggtaacg
      481 ggagctcatt agagaaaacc cctttcatca aaataatctc gactgtaaat ttcttacaat
      541 catcatcgag ttttaacaat ccatctaaaa tcttctctgt ttagccgtaa attcctgcca
      601 attgccgtct aataaaattg ctgaggaaac aagggtgtct tttccgcgga ggagagagaa
      661 taacaaagga aataaaatat catgcctgga tgagggatgg atttggagca agaaagcaag
      721 aaaactgagg agcggccttt tatttttcct tgggggagtc gccgttttgg ccttgccggg
      781 ttggtcatcg gcttcctttt ggctttgggc gtccggattc catggctctg ggagatcccc
      841 aggttcattg atgaactacg ggaggtgaat ctggcttact tgatctcgag gggccaggcg
      901 ttgccactcc acaacgcggc gcatgatatc ggagccctgc acaattatat attggccggg
      961 atttttgata tatttggcac cggaatatac ttgccacgtc tttatacagc tgtaacaagt
     1021 gctatgacag tgatattcat ttacttgtca ggttccagac tttatggccg ggttgcgggg
     1081 gtagtcgccg ctggattgct tttaaccaac ggaatgcata tcctggttac tcatatggct
     1141 tgggcaaatt gtaccacccc gttctttttt acgttcgccc tatataccac tatccgagcc
     1201 gcggaacaac cgagcggggg ttggctcgtc ggggccggtt gcagctgggc gttggccctc
     1261 cagactcatc cgtccatcat catttttttg ccggtagtcc tattttatat tgcccgtact
     1321 cccggactgc aacctctatt tagtcgtcgg tcgggtgttt cggttcgtta tcattacttg
     1381 gccgttcttg cttttgcagc cggttacagc aatatgctta tttacaatct agtctcccgc
     1441 ggcggaagta ttcgttgggc gatgcaaaaa ggatatacct tggagcagtc acccggtccg
     1501 gtttcatttg tccataacct cattcaaatg tgggtggaac tcatacggac aatcggatcg
     1561 gtatatcaaa ccaaatccgg tatgctggaa tatttggcgt atccagcttt tagcgccggc
     1621 ttattcatgt tgggatatgg catttacagg gcagtacgat gccgccgtgc tcttccagtc
     1681 tggatgcttt tggccggttg cactgtaatt ccttggatta atggacgtta cgggtattat
     1741 atcgcaaccc ggtacattat gccgctggtc atctgtgctt tgctcttgat tggctgcgcg
     1801 gcgaatgatc tatggaaatt acaacagcga tggttgtcaa ggcggtggcg gcctattcac
     1861 tttgcaggaa gtgtgttggc acttggagca attattcttt ggcagcaggg tcttttctat
     1921 gactattgcc gccggcttgc ggatacggat atgtccaacc gaatggcgct tcagatcgta
     1981 gagcaaacaa ggcaaatggc tggtttggat agaagaggac ttcgagtgaa ccgaagaatc
     2041 gagatcctga tcgatgacag agtacatatt ccaaatgacc cgcttcccgt attataccgt
     2101 ttagccggat taccttatac ggtgttgttc cattcaagcg gagaaagtga ttcggctctt
     2161 cgaatccgtt ggcttgggaa gttgggatcg aatccgggaa tccgccggat catggtttta
     2221 gatgaacaga catatcggaa gttgagggag tttattcctg acgctcgcgt agtacgattt
     2281 gaaacccgga aggttttaca tgggacccat ggtcaaattc gtaccacggt tttcctcgca
     2341 agtcgggttt ctgattgaat gagatcccat gttttcctgg tcgtggtcat ggaatgtatt
     2401 cccgaaaacc gggaaccaaa ttactttaaa tacgtacata tttgagtggg gatatgaaaa
     2461 ctgtaaaatc attggtgatt tatttattaa attgtctttt atcctccggt gacaccgatc
     2521 cgacttttta ttccgggcgg ccaagggcaa cacgcgtgcg acttaacaaa cgataaaggg
     2581 gtatcaaggt ggccaaatta cacggtatca tgatctgcgt tacccgacaa aaaacctgcg
     2641 aacggctgat tcgattcggt tatgaactgg ctcaaaaaga cggggcggat ctttatgtgg
     2701 ttcatgcagc caaagtagga gagaactatt tggggaatcc caatgaaggg gaagccctca
     2761 attatctgtt ccaagtatcc aaacaggtgg gcgcggagat gaatgtgatc cgttccaaac
     2821 atgtcaccaa gacactggcc gatttcgcca ggaaacagaa gattgccatt atggttttgg
     2881 gagcggcccc cgattccgcc agtgataata agatcattca agagcttcag ctccatctgc
     2941 cacagatgga tttcaaagta ttgcctacgg cataaattga acccccagga attttttcgg
     3001 ctgttggatt gtagtcatcg tattcataga cactagaaca tgtaaacaat catatcccgg
     3061 atgccgatat aaataatagg gcgcgcgcgc ttatgcgcga ctttacacta ccatagagag
     3121 gagacgaatc gttatggatt cggatatgat gggtatagcc agcgcctcaa ccgggatgaa
     3181 gcagaatcag attctgatgg cggtccagac ccagatgctc cggaccagca tggataacca
     3241 ggcccaggcg gtgcagagtt tacttgaagc attgccggta gcgggtccgt cccatctggg
     3301 gactaagatc gatatcacag tctgaaccga ttcccggctt tttgaacctt gattcgggca
     3361 cggattatct atctcgccga actgggttca actgaaatga tccggttttt accggatcat
     3421 ttttttatag aaaacctctc tttgggtctg ttccacagcg atttccgtag aaattcccgt
     3481 taaatctttg aacacgatga aaggcttgat tttgcgtctt ttcgaaaaac catcataatc
     3541 caagaaagat aattatctct gatttggtta ggcttcactg gtttgacaga ttgttatcaa
     3601 aggaatgttg agtgatatcc agaaaaccgt agtgcaaaag gttgtttaag cccgggagga
     3661 actatcttca aaccgcgctt atccatccaa agaccttgaa agggagtgca ttcaatgaag
     3721 aaattcatcg cagtaatggt aatgactttg atcctgattg gttcggccgc atccatcgtc
     3781 cttgccaatc caatgagctt aagggctgaa aatggtctgg tcatgccggc gagcggtctc
     3841 aatctcgact tgggaaccgg cttcgcgctt cacaattcct cttccaccgc aagcacacca
     3901 tcccttgagg ctcagctcaa ttacggcgct tttccggggc tgactgtcgc tgcggcaatc
     3961 gaggctccaa tgaacgtcgc cagcagcaat gatgttcaaa agatggtaaa attgtattac
     4021 agtccgatcc acgatgggcg gggttacacc ctttatctcg gctatgatct ggacaaagcg
     4081 acgataccgg tttatggcct ctcgctttgg gccgactcca agctactttt gggttatatc
     4141 aacctggaag cccgttcgga gaatgaagga acggcagcgg taatgattac tcccggtgcc
     4201 aatctgcaca tcggctccaa attacagatc ggcggcgagg ttgaagtgga accggaaaac
     4261 tggcagacgg gcgaattcac tcttggtgcg agttacgcag tatatcccaa agtacagctc
     4321 aaaatgggcg tgatcaccgg gggagagtcg ttcatacccc aatcatacaa tctgggcctt
     4381 tcggcaaccc tgtgaattcc gtttgaccaa ttcaaagact gtgactaaaa aagaaagcgc
     4441 tccggagagc gctttctttt ttgatcaacc cgaaattcat ttcccggttt taccgggctc
     4501 gaggcctgtt gtaatctttt gaagcatcgg ttgcaattgt accagggctt gttgggtttc
     4561 ttccggcccg gttagggata gtttgattga acctaatttg gtccggatta acctggcggt
     4621 atctgcagag gtaaccccgg caagcagctt ggaataggca tcgtaatcgc tcaaccatgt
     4681 ctggttaaag aaagcttgca aggccaggtt ttgagtggcg gttcgcctcg agtcgccaat
     4741 acgtttggcc tgtttttcca ccgcctgttt aagtgaggtt aattctgctt cggggatacc
     4801 attcttggca ataccttgta atactgagat cacttggccg atggcgggtc cggcattacc
     4861 gggtagtgtt tcgaattcga ttaacaagat tggggccaca ggttcggtca ggtaagaatg
     4921 gaaaaccgaa acatcataac agtaaccggc ttgttcccgg agtttattcc ataactccca
     4981 ggctagatat tgctgaacca gtaatgatgt agccagatca ggatctgtca aggccggggc
     5041 cggcaagcca ataaatactg aagcagtgct attatgaacc gtggtaatct caacaagtgg
     5101 gttgggactg caggtggcag caggaaccgg gctttttgtg atcacttgct tcatctccct
     5161 gccgctggga cggagtaaaa agtcatccgg tagcggggcg gtacttgaga tgattaggtt
     5221 ggacggccgg tagattcggc ggaaccaatt gttggcatcg gtgatatcca ggttggacat
     5281 atagttatca aagattttgg tataaggatg accggccccg tctatcatgg tatagagttt
     5341 ggccagaccg acctcactgg ggtttcgcgg caagttccgg taatttttta gcaatatatt
     5401 gcactcgtca taacttaaca aggcctctat tcgaatcacc gcatccagtt gccggcagaa
     5461 tgcggtaaag tcattgttgg gtaccgcgaa gtcaaaccgg gaataatcgg cggctagaaa
     5521 ataattgaag tccaataagg tcgccatttg aagaatccgg taaaccagga tattggtcaa
     5581 gtcggtcaaa cccgccttag cggcaggatc ctgactgtaa ccggttttga tcagtaaagt
     5641 cagatattgt acttcgcttt cagccccgga accgttgaca atggtggtca acccgtcgtc
     5701 ccgaacggcg atggtgcctg acgttgtttg attttgggct gttgccagtc cgggtatcag
     5761 agttagcccg agcatcacaa ccagcaacca cggtaaaaac cgtctttggt cggaaaatgt
     5821 ccttcttggt ttcatacttt ttcccatcct tatctctgcg cgagcgcgcg ccaacatcga
     5881 aaatactcgc tttggtgagg acttcctatg attcatctca tgcgaaatca tttggcgaac
     5941 tacaaagcct taatcacgat tttttggcta tattgacctg ccaggtattt gttgacaaac
     6001 ttccggatat cgcctgaatt gatgtagcgg atggtttcca gatcccggac tgtaatggcg
     6061 gcgggatcaa acaattgcgc gtcgtaatac tcgttcaacc cgtaggcatc ctcgctcaaa
     6121 ctatccaggt accgaccttg agttttagcg aaccaggata gaaagttgtc caacttttta
     6181 gctgcca
//
LOCUS       DASVMB010000070         7259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_155403,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000070 DASVMB010000000
VERSION     DASVMB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155403"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..2863)
                     /locus_tag="VF531_02195"
     CDS             complement(179..2863)
                     /locus_tag="VF531_02195"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7712806.1"
                     /translation="MSLATVYEPQSVEEKWYQFWLEHHYFHAEVEHDRKPFCIVIPPP
                     NVTGVLHLGHALDETLQDILIRWRRMQGYNALWMPGTDHAGIATQARVEEALAKEGIS
                     RYDLGREEFLKRVWSWKEKYGGTIIRQLKSLGSSCDWERERFTMDEGCSAAVREVFVR
                     LYEKGLIFQGSYLINWCPKCQTTISDIEVEHEDREGHLWYIKYPLADGSGFVEVATTR
                     PETMLGDTAVAVNSEDDRYRNLIGREVILPLADRRIPIIADAYVDMSYGTGAVKVTPS
                     HDINDFEMGLRHQLPQITVIGFDAKMTAAAGKYAGLDRYQCREQVVTDLERLGLLDKI
                     ENLTHAVGQCYRCDTIIEPLISKQWFVKMQPLAEPAIQAALQGDVSFIPERFTKIYLG
                     WLENIRDWCISRQLWWGHRIPVWYCQDCQEVIVSRNDPDRCPKCGGSHLEQDPDVLDT
                     WFSSALWPFSTLGWPKQTPELEHFYPTSVLVTGRDIIFFWVARMLFMGLEFQGCVPFK
                     DVLIHGLVLDKFGKKMSKSRPETIVDPQDIIAKYGADTLRFTLATGTALGQDQRFQME
                     KVEGSRNFTNKIWNAARFVLMACEKDTPDDSQDGSNWLDFASGRSDVIPSGLTLPDQW
                     ILSRSGRLTSEVTRLLERYDIGAAASAIYDFLWNEYCDWYIELAKPRLNQTADVNDRR
                     TVQAVLVRVLRQTMELLHPFMPFLTEEIWQALPHRDESIMIASWPQNLTEQQFPEAET
                     TMGTIIEVVRAIRNIRSEASVPPQKKVSAYFAADDEQRAILKSNQSYVTALAGLENYQ
                     LLNPTDPKPEKAISAVVHGITIYMPVAGMVDVVREQERLTKELQQLQVELGKLKDRLN
                     NPAFSAKAPPQVVEKEREKMGIFQDKIDKIKLRLDQLV"
     misc_binding    complement(2909..3142)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3238..3810)
                     /locus_tag="VF531_02200"
     CDS             complement(3238..3810)
                     /locus_tag="VF531_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712807.1"
                     /translation="MYFVWLFLVAFLLIFFFCTNWPVRMYHNHYLAKLAAILGSIVQN
                     RHSSIMGPAPELNTIYHGRPFTIRFVEGSVDALQSNSGLEIRMEIKSEAVMECYRLSG
                     KSRERGEFIRFQTGTPEIDSRWFIMTTNPTTAASFWEKSGLKELLAFNQRNIEQLSIN
                     RTEMIIRLQKNSISQVVELVNRLVGLTGCN"
     gene            complement(3876..4727)
                     /locus_tag="VF531_02205"
     CDS             complement(3876..4727)
                     /locus_tag="VF531_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712808.1"
                     /translation="MNEKPVLAFVFSLFIVILTAAVTYMGLFIPEIYGDRATELVLSL
                     QARDTVLLILVVPLLLVTACLALRKSRRAYLAWLGILAYLSYTYAGYSFGLANNPLFL
                     IYVIIFSLSLFSLVLNLNRLNIEAVPRWFSPKTPVGGVALWTTVSGLTVGAIWLRQVL
                     PGLINVQNTRILTLGVDPGLAIPALDLGLVAPLAVFTGIWLWKRRAWGYTFACLILFQ
                     TIAIGLNHLSYEWWLSQRGTVSDSVQLLIYGGLVMIPLLLVITFLRNLREEILKNNSD
                     RSTVSYF"
     gene            5017..5481
                     /locus_tag="VF531_02210"
     CDS             5017..5481
                     /locus_tag="VF531_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7712809.1"
                     /translation="MNNQLPIFEDLLNRSFTSLSRLEETHLKQSLHQELTVNDFHIIQ
                     SIGLYTERTMSELAKILAITPGALTPAVDRLVKKGFIARNHNDQDRRVILIKLTTRGE
                     AAFREHARFHAQITQKLVEDLTLEEGMALLKALEKLADSVTSQATESGTPSV"
     gene            complement(5563..6558)
                     /locus_tag="VF531_02215"
     CDS             complement(5563..6558)
                     /locus_tag="VF531_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712810.1"
                     /translation="MKRTIWSGFILLGLVLLGMFLAFSEAASPKKITIAMTYKPSVQF
                     APWYVAAEKGYFREAGLAVNFDYRMDIDALPLVAQGQMDFAIAGGDQVITARAQGIPV
                     DYLMSLYAKFPPTVVSLSDSKIRTPQDLKGKRIGLPLYGTSLLAVKAILNKAGIKESE
                     VELVDIGYTQIPALTSRKVDAVVGFANNEPLILRQLGYKINEIRSWDYFSLVGHGLIT
                     GEQMIKTSPDLVRKMVGATLQGMRYTLAHPEEAFQLSLKYLPELGKDQREQEHQVFLA
                     SIKLWENDYTRRKGLGFSDPRAWEDSQRLMAQAGMIPRETKVNEFLRQEFLTAEQ"
     gene            complement(6702..>7259)
                     /locus_tag="VF531_02220"
     CDS             complement(6702..>7259)
                     /locus_tag="VF531_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712811.1"
                     /translation="GFIAGTFLALPLSYFLARFPWLEKLFAPYIVGLQAVPIVALAPL
                     IVIWFGFGLSSKILVAALVAFFPVLTNGIVGFRSTNPRFRELMAMLGGTPGQVFWKLE
                     LPSALPVLFGGLKLGITLSVIGAVVGEFAGAGRGLGYLVNNARGSFDTPLIFVAIIFL
                     AMLGVGFYALISLVESLCMPWKRKK"
BASE COUNT         1951 a         1924 c         1645 g         1739 t
ORIGIN      
        1 ccttaggtcg agcatttact tccggcttcc ttcagattcc gtctcgcaac ggacaccctt
       61 gccctcggtt aacggtaggc gctcgccagc ccccgttcgg gactctcacc ctatagtcaa
      121 catccatgcc gggcgtacaa agaaaagaac ccttcacaat gaagggttct tgaataacct
      181 agaccaactg gtcaaggcgt aacttgattt tatcaatttt atcttgaaag atccccattt
      241 tttcccgttc cttctcgaca acctgcggcg gagccttggc actgaaggcc gggttgttca
      301 agcgatcttt cagtttaccc aactcaacct ggagttgttg gagttccttg gtaagtcgtt
      361 cttgctccct cactacatcc accattcctg ccacgggcat atagatggtg ataccatgta
      421 ccaccgcact aatcgctttc tccggtttgg gatcggttgg attcaggagt tgataattct
      481 cgagtccggc caaagctgta acgtaacttt ggttcgattt taaaatcgcc cgttgttcgt
      541 cgtcggcagc aaaataggcg cttactttct tctgtggcgg cacacttgcc tcggatcgaa
      601 tattacggat cgcccgaacg acctcgatga tcgtccccat tgtggtttct gcctcgggga
      661 attgctgttc cgtgagattt tgaggccagg aggcgatcat aatgctctcg tcgcgatgcg
      721 gtaatgcctg ccagatctcc tccgtcagga acggcatgaa tggatggagc agttccatcg
      781 tttgacgcag cactcgtacc aaaaccgctt gaacggttcg gcgatcattt acgtcagccg
      841 tttgattcaa acggggtttc gcgagttcga tataccagtc acaatattcg ttccaaagaa
      901 aatcataaat ggctgaagct gccgcaccga tatcgtaacg ttcaagcagc cgggtgactt
      961 ccgacgttaa ccgtccgctg cgagataaaa tccattgatc gggcaaggtc aaacccgaag
     1021 gaatcacgtc gctccggccg ctggcgaaat ccagccaatt gctgccgtct tgcgagtcat
     1081 ccggggtatc tttttcacag gccatcaata caaatcgggc tgcattccaa atcttgttgg
     1141 taaagttacg gctaccctcc actttttcca tttggaaccg ttgatcctgg ccgagtgcag
     1201 tcccggtggc caaggtgaaa cgtagcgtat ccgcgccgta tttggcgatg atgtcctggg
     1261 gatccacgat cgtctccggc ctggatttgc tcattttttt accgaactta tccaaaacca
     1321 atccgtgaat cagtacgtcc ttgaacggca cgcagccctg aaactcaagg cccataaata
     1381 acattcgcgc cacccagaaa aagatgatat cgcgaccggt gaccaaaacc gaagtaggat
     1441 agaaatgctc gagttcaggt gtttgtttcg gccaacccaa agtggaaaac ggccataaag
     1501 cggaggaaaa ccaggtatcc aagacgtccg gatcttgctc caggtgactt ccgccgcatt
     1561 tggggcaacg atcgggatcg ttcctggata caatcacttc ctgacaatcc tggcaatacc
     1621 aaaccggaat ccgatgtccc caccaaagtt ggcgggagat gcaccaatcg cgaatgttct
     1681 ccagccatcc aaggtaaatc ttggtaaacc gttccggaat aaacgaaacg tcgccctgga
     1741 gcgcggcttg aatcgccggc tcggccaagg gctgcatctt gacaaaccat tgtttggaga
     1801 tgagcggttc gataatggtg tcgcagcggt aacattgacc caccgcatga gtcaggtttt
     1861 caatcttgtc caaaaggccc agtcgctcca gatcggtaac tacctgttca cggcattgat
     1921 aacggtcaag tcctgcgtat tttccggcag ccgccgtcat tttggcatca aaaccgatca
     1981 ccgtgatctg cggcaactgg tggcggaggc ccatctcaaa gtcattgata tcatgagacg
     2041 gcgtgacctt gaccgctccg gtgccataac tcatatccac gtatgcatcg gcgatgatcg
     2101 gaatcctccg gtcggctaac ggtaaaatga cttccctacc gatgagattc cggtaacggt
     2161 cgtcttcgga gttgactgca acagcggtat cgccaagcat cgtctccggc cgggtggtag
     2221 ccacctcaac aaaaccggag ccatcagcca gaggatactt gatataccag agatgccctt
     2281 ctctatcttc atgctccact tcgatgtcgg aaatggtggt ttggcatttg gggcaccagt
     2341 taatcagata actgccttgg aaaatcaagc ctttttcata gagccggaca aaaacctctc
     2401 ggaccgcggc tgaacagcct tcgtccatgg tgaaacgctc ccgttcccaa tcgcaggatg
     2461 acccaaggct ttttaactgg cggataattg ttccaccgta cttttccttc caggaccaaa
     2521 cccgtttcaa aaattcttcc cggcccaaat catatcgcga aattccttct ttggccaggg
     2581 cttcttccac tctggcttgg gtagcgatgc ccgcatgatc ggtacccggc atccacaggg
     2641 cgttatagcc ttgcatccgg cgccagcgga tcaggatatc ctgcagggtt tcgtccaatg
     2701 catggcccag atgtaaaacc ccggttacgt tgggcggcgg aatcacgatg caaaaaggtt
     2761 tgcgatcatg ttcgacttcg gcgtgaaaat aatgatgttc gagccaaaac tgataccatt
     2821 tctcctccac actttggggt tcataaaccg ttgctaaact catctgctta acctcctgaa
     2881 ttcatgcaaa taaaaaaaga ctctcatcct ctaggacgaa gtctttatct ccgcggtacc
     2941 acctaacttc cccgtgcggc caagcgcaat cgggctcttg atcgctgtaa cgggcgaacc
     3001 ggccggcatt acttgataat caatcgatca tcttcacacc ggctgctccc gggcgacctt
     3061 caacctcttc gggcagggcc ttgcaccagc cggcccctcg ctaagccctc caaagtttac
     3121 tcctcccgat cctcgcgata tataaacttg tcatgattat acaatcactt ttaaaggatt
     3181 gtcaagagcc aggcggaatt tctcctccga aaaacaatcc gaatcaatca gtaaacttta
     3241 gttgcaaccg gttaatccaa ccaaccgatt caccaactct acgacttggg atatggaatt
     3301 tttttgcaac cggatgatca tctcggttcg attgatcgag agttgttcga tgtttcgctg
     3361 gttaaacgcc aacaattctt ttagaccgct tttttcccaa aatgaagccg ctgttgtcgg
     3421 attggtagtc ataatgaacc aacgggagtc aatctccgga gttccggttt gaaagcggat
     3481 gaactctccc cgttcccggc ttttaccgga aagacgataa cattccatca ccgcttcgga
     3541 tttgatctcc atccggattt ccagaccgga attggattgc agggcatcca ccgaaccctc
     3601 gacgaaccga atcgtaaacg gccggccatg atagatggta ttcagctcgg gagcaggacc
     3661 catgatcgac gagtgcctat tctgtactat gcttccgagg atcgcagcca gtttagctaa
     3721 gtaatgattg tgatacatcc gaaccggcca gttggtacag aaaaaaaaga tcagtaaaaa
     3781 agctaccaga aaaagccaga caaaatacat taacagctgc ctccagtcaa ccaagaaaaa
     3841 cctttcctgc ggatcaaatt atgcatcctg cttggctaaa aataagaaac ggtgctgcga
     3901 tcactattat ttttcaggat ttcttcacgt agattccgta gaaatgtgat tacgagtaag
     3961 agcggaatca tgactaaacc accatagatt aggagttgca ctgaatccga aactgtaccc
     4021 ctttgcgaaa gccaccactc atagctaagg tgattcagac cgatagcgat ggtttgaaag
     4081 aggatcaggc atgcaaaggt atatccccaa gcccggcgtt tccaaagcca gattccggta
     4141 aaaacagcca atggagcaac caaacccaga tccagtgccg gaattgccaa ccccggatca
     4201 acaccgagag tcaaaatccg ggtattttga acattgatta aacccggtaa aacctgacgc
     4261 aaccagattg ctccaactgt aagcccgcta acggtagtcc agagcgcgac cccgccaacc
     4321 ggggttttcg gcgagaacca gcggggcacg gcttcaatat ttagtctgtt caaatttagc
     4381 accaatgaga agagagaaag tgaaaaaatg atgacataaa tgaggaataa cgggttattg
     4441 gcgagtccga aactatatcc tgcatatgta tacgagagat aggccagtat tccgagccag
     4501 gctaaatagg cacggcggga tttgcgtaac gccagacaag cagtgaccag caaaagtggt
     4561 acgaccaaaa tcaataagac cgtatcccgg gcctgcaatg aaaggacaag ctccgttgcc
     4621 cggtctccgt aaatctcggg aataaataat cccatgtaag tcacggccgc agtcaagata
     4681 acgatgaata aggaaaaaac aaaagccaat accggtttct cattcaaccg caatctcccc
     4741 cttttgcatg aattataact ccggaattat ccgaatggat tcgaaaaagc cagaatccgt
     4801 taggccttaa ggcagcgacg gctttgacaa aagggatgcc gccggaatat aataaaataa
     4861 aatattttag ttactaaact ttttgaatcc cgtacttttt ggtaatgcca cggtcagttt
     4921 caaaatccta tattttcaag gtgtttcccg gaaaggtctg cacccatcgc tatcgggacc
     4981 tacaaaagaa ctctgcctgt gccttcggag gttttgatga acaaccagct gccaatcttt
     5041 gaagatttgc tcaaccgtag ctttaccagc ctaagccggt tggaagagac tcatcttaaa
     5101 caatcccttc accaggaatt gacggtgaat gattttcata tcattcaaag catcggtcta
     5161 tataccgaac gtaccatgtc ggaactagcc aagatactcg caattacccc gggggcctta
     5221 acccctgccg tagatcgcct ggtaaagaag ggctttatcg cccgaaacca taacgatcaa
     5281 gatcgccggg tgatcctaat caagctgacc acccggggag aggcggcttt tcgtgaacat
     5341 gcccggtttc acgcccagat cacccaaaaa ttagtcgagg acctgactct tgaggaggga
     5401 atggcgctac ttaaggctct ggaaaaactg gccgattccg ttaccagtca ggcaacggaa
     5461 tccggcaccc cttccgtttg accttatggt taatgattat tctgctcttg ccagcgtttt
     5521 cctgccggtt tcccgagaaa accgccaaaa ttcttgccaa atttattgtt cagcagtcag
     5581 aaattcttgc cgtaaaaact cattcacctt tgtttcccgc ggaatcattc ctgcctgcgc
     5641 cataaggcgt tgcgaatcct cccaggctcg aggatcggaa aatcccaatc ctttgcggcg
     5701 cgtgtaatca ttctcccata attttattga agccaaaaag acttgatgct cctgttcccg
     5761 ctggtcttta cctaattccg gcagatattt taaggatagc tgaaatgctt cctcgggatg
     5821 ggccagggta taccgcatcc cctgcaaggt ggcgcctacc atcttgcgta ccaaatccgg
     5881 ggaagtttta atcatctgtt ctccggtgat taacccgtgt ccaaccaggg agaaataatc
     5941 ccaggagcgg atttcgttaa ttttatagcc cagctgtcgc aatataagcg gttcgttatt
     6001 ggcgaatccg actaccgcat ccactttgcg gctggttaaa gcgggaattt gggtataacc
     6061 gatatctacc aattccacct cggattcttt gatcccggcc ttgttcaaaa tcgctttcac
     6121 tgcaagcaaa ctggttccat ataacggcag gccgattcgt ttccccttta aatcttgggg
     6181 agtccggatc ttggaatcac tcaacgaaac caccgtggga gggaatttgg cataaagact
     6241 cattaaataa tcgacgggaa ttccttgggc tcgtgcggtg atcacctggt ctcctccggc
     6301 gatggcaaaa tccatttgac cttgagccac gagaggtaaa gcatcaatat ccatccggta
     6361 atcaaaatta accgccaaac ccgcctcacg gaaatacccc ttttcagccg ccacatacca
     6421 tggggcaaac tgaacgcttg gtttataggt catggcaatg gtgatcttct tagggctcgc
     6481 ggcttcggaa aaggctaaaa acattcctaa taataccagt ccgagcagga tgaacccgct
     6541 ccaaatcgtt cttttcatca aaatcaacct ccttggaatt taaaatgaac cacgaaacac
     6601 acggaattca cgaaatggct gtgtgcatgt aaatgttgaa caagaaacat atgcacacct
     6661 ttccattctt gaatagcatt caactaataa cgaatcacta atcacttttt tctcttccat
     6721 ggcatacata aggactccac cagggaaatc aatgcgtaaa atccgactcc gagcattgcc
     6781 agaaaaataa tggccacaaa gattagcggt gtatcgaaac tgccgcgggc gttattgacc
     6841 aaatatccca atcctcttcc ggcacccgcg aattctccaa caactgcccc aatcaccgat
     6901 agagtgatac ccaatttcag acctccgaat agaaccggca gggctgaagg tagttccaat
     6961 ttccaaaaaa cctggcccgg tgtgccccca agcatagcca ttaactcacg aaaccgagga
     7021 ttggtgctcc ggaaaccgac gataccattg gtgagaaccg ggaaaaaagc caccaaagcc
     7081 gcaaccagaa tcttggagct aaggccaaag ccaaaccaga tgacgatgag cggagccagg
     7141 gcgacgatgg gaaccgcctg cagcccgaca atatacgggg caaatagttt ttcaagccac
     7201 gggaagcggg ctaaaaagta actgaggggt aaagccagga aggtaccggc gatgaaacc
//
LOCUS       DASVMB010000071         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_155406,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000071 DASVMB010000000
VERSION     DASVMB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155406"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1521)
                     /gene="murC"
                     /locus_tag="VF531_02225"
     CDS             complement(172..1521)
                     /gene="murC"
                     /locus_tag="VF531_02225"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7712812.1"
                     /translation="MRHLHFVGIGGARLRGLAKIYADRGYVISGSDWQESRETKHLME
                     RGIKVYLGHDPANIEGADMVVYTNAVGNENPEVLEARKRGIPTMEGAELLGKLMSDVG
                     YGIAIAGTHGKTTTSAMTSLLLAAGGLDPTVLIGGELGAFQGNHRSGQSKLIVVEACE
                     FKRSFLKLHPSLELITNIDWDHPDCFPTLGDVVKTFEDFVALLPRDGKLVIWGDDPNA
                     SQICRQFPGKTIKFGFEKNFDWSVNGFTPVPPLGVNAPLFYRGKKQGELNLKVPGRHN
                     IQNALGALAIATEMGVDLQTVLKTLADFRGVQHRFEIKGEFNKVMVVDDYAHHPGAIK
                     TTLAAARQFFKGRIWCAFQPHLYSRTKYLLPEFAQAFTDSDILVLADIYPAREKDPGD
                     ISSRDLAREAQKFHKDVRYIGDFDSIYQHLQANLAPGDLLITMGAGDIWKVGERLIR"
BASE COUNT          473 a          545 c          463 g          488 t
ORIGIN      
        1 ccttaggtcg agcatttact tccggcttcc ttcagattcc gtctcgcaac ggacaccctt
       61 gccctcggtt aacggtaggc gctcgccagc ccccgttcgg gactctcacc ctatagtcaa
      121 catccatgcc gggcgtacaa aaaaaggtaa gggcaggttt gaaacccgcc cttaccggat
      181 tagacgttcg ccgactttcc aaatatcccc cgcacccatg gtaatcaaga gatcccccgg
      241 ggccaggttg gcctgaagat gctggtagat agaatcgaaa tcaccgatat aacggacatc
      301 tttgtggaat ttctgggcct cgcgggccaa atcgcgactg gagatgtccc ccggatcctt
      361 ctcacgggcc ggataaatat ctgccaaaac taaaatatcg gagtctgtga aggcctgggc
      421 aaattccggc agtaaatatt ttgtcctgct gtataagtgg ggttgaaacg cgcaccagat
      481 tcggccttta aagaattggc gggctgcagc caaagtcgtc ttgatcgcgc cgggatgatg
      541 ggcgtaatca tcgactacca ttaccttatt aaactcccct ttaatctcaa agcggtgctg
      601 tactccgcgg aaatcggcca gggtctttag gaccgtctgc aaatcaacgc ccatttcggt
      661 ggcaatggcc agtgctccaa gtgcgttttg aatattatga cgccccggca ctttcaagtt
      721 aagctcgcct tgtttcttgc cgcggtagaa taatggggca ttgactccca acggcggtac
      781 cggggtaaaa ccattcaccg accaatcaaa gttcttttcg aaaccaaact tgatcgtctt
      841 gccgggaaac tgccggcaga tttggctggc attggggtca tcaccccaga tgactagctt
      901 cccgtcccga ggcaacaacg ccacaaaatc ctcaaaggtt ttaaccacat cgccgagggt
      961 gggaaagcaa tcgggatgat cccaatcgat gttggtgatc aactcgagcg acgggtgaag
     1021 tttgagaaat gagcgcttaa attcgcaggc ttccactacg atcaacttgg attgaccgga
     1081 gcggtgatta ccttggaacg cgccgagttc ccccccgatc agaaccgtgg gatccaatcc
     1141 tccggctgct aaaagtaatg atgtcatggc ggaagtagtg gttttcccgt gggttccggc
     1201 aatggcgatc ccgtatccga catcgctcat cagcttgcca aggagttcag ccccttccat
     1261 tgtgggaatc ccccgttttc ttgcctcaag tacctccggg ttttcattcc cgaccgcgtt
     1321 cgtatagaca accatatcgg cgccttcgat attcgccggg tcatgaccta agtaaacctt
     1381 gattccccgc tccatcaagt gtttggtctc ccgagactcc tgccagtcgg aaccggagat
     1441 gacatagcca cggtccgcgt atattttagc caatcctctc aggcgggctc cgccaattcc
     1501 tacaaaatgg agatgtctca ttaccctaaa tagctcccct ctatgctgat tactccggtg
     1561 gcaaaaggaa tcccccgttc caatcggacg atgttttcca tttgccatca gtgtaacact
     1621 ttcacgcaat cgtggatttt caagcaaaac ccccaatttt cactcataca ttataacaca
     1681 tttcctaaag ataattataa attcttgagt aaccatcgat ttccggtttg gccccggttt
     1741 acgatgatgt tgtgcgcacg tgtacgtgcg cgcgcacact tagtatattc ttaccatatc
     1801 cattcatccg ctggcctttt gccaggtagg acagtatcaa gccaactgtt aattccgtga
     1861 aacccggaaa taataaaaac gggagtttcg cgcgcatacg cgtcgagctt ataagcgcgc
     1921 cctaactccc cgggaggtga aataatgagt cgccgtgata gcatcgttt
//
LOCUS       DASVMB010000072        24791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_597145,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000072 DASVMB010000000
VERSION     DASVMB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 24791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24791
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597145"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="VF531_02230"
     CDS             <1..1742
                     /locus_tag="VF531_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HEX7712813.1"
                     /translation="DRYYHHPQDFQVDPGEWQELEQAFNRGFSTGYAGGNRNHELMSF
                     TRPNNRGVYLGRVAFFDKLQRRLRLKLEADLEIGDEVEAWVSQGGRAVSPVRELFRDG
                     RKIDQAPRGSVVELTIDHKVFPGDRIFKVFSIQNDRRLKQGLAPDSVALKIPCNAEVN
                     GNAGGALRITLRDATGNRGTALSDTPLQPAKNRPLTRDVLEEQLGRLGNTPFRLGGLE
                     ISLPENVMLPIGELNQLRRKAIQELTAKKLDIYRRPRVKLPDSLSLTAGPVKFDGSQA
                     EKSSGSVPVQSEGRLLSVWVGDLEGVAAAAGEGAALIYAGGDELTGFHWDEKSFREAI
                     DIAHLSRAELVVGLPRINREGQRGIWEQCLELALGLPADGIMISDLGTLQRVQEYGTG
                     PLYLNYPLNCFNSAALAELAGPRIAQITISPELTLEQISGLKTGIRLECLVQGPLELM
                     VSEYCPLHTVLGKTGACPGLCSNRGQFALRDRLNLDFPIYPDQYCRMHLLNSVDLCLY
                     ADLDHAAFSGLVWRLELKTLPAQKVAAIVRLYRQVANDPGIAARHGDIVLDQLKELTG
                     RGITKGHYFRGVE"
     gene            1930..2196
                     /gene="rpsO"
                     /locus_tag="VF531_02235"
     CDS             1930..2196
                     /gene="rpsO"
                     /locus_tag="VF531_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7712814.1"
                     /translation="MLTADKKHDLISKYGLHEGDTGSPEVQIAILTERINYLTEHLKV
                     HKKDHHSRRGLLKMVGQRRGLLNYLSEKDITRYRAIVERLGLRK"
     gene            2399..4507
                     /gene="pnp"
                     /locus_tag="VF531_02240"
     CDS             2399..4507
                     /gene="pnp"
                     /locus_tag="VF531_02240"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7712815.1"
                     /translation="MQKWEMTLGGRPLVIEYGTVAKQANAAVLVRFGETVVLVTAVMS
                     DKIREGIDFFPLLVDYEERLYAVGKIPGGFIKREGRPAESAVLAARTIDRPIRPLFPE
                     GFRNDVQVAATVLSVDPDNEPSLAALIGASAALHISEIPFAGPIGGVKVGRVNGEYII
                     DPTVAQQEKSDLQLVVAGTKDAIMMVEAGANEISEDDALEAILYGHSVIREIVAFIER
                     IRADVGKPKIEPVLDEPMPDIDRMVRDFATAKMAVAIKNGDKLEREAHIEEVKEEARQ
                     YFQTQMGETYAANLKYINMVLDNLLKEEVRKMIAVEKIRPDGRKLSEIRPISCEVGLL
                     PRAHGSGLFTRGQTQVLTVCTLGRVSEEQILDGLGEDDSKRYIHHYNFPGYSVGEVRP
                     VRSPGRREIGHGALAERALLPVIPNEEEFPYTIRLVSEVLESNGSSSQASVCGSTLSL
                     MDAGVPIRKPVAGVAMGLLKDGEDFAILTDIQGLEDHFGDMDFKVAGTKDGITAIQMD
                     IKIKGIGREILKQALAQAREGRLFILDKMLSTISQPRTELSAYAPRIIRFSIDPDRIR
                     DVIGPGGKIIRKIVEDTGAEIDIEDDGRVFIAAVDASAGTKAQEIIKRLTSDVEIGAV
                     YLGKVTKLMNFGAFVEVLPGKEGLVHISQLAKERVAKVEDVVNVGDEIMVKVIEIDKQ
                     GRINLSRKAVLTDETPVAVH"
     gene            complement(4836..5888)
                     /locus_tag="VF531_02245"
     CDS             complement(4836..5888)
                     /locus_tag="VF531_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HEX7712816.1"
                     /translation="MSSSQVKPESLKMPSITAIMAGFGLKVLSYETYRGIWRVQTEIG
                     YKYLKRSKLNQEEVRFIFEVLEYLNGRGFRRAPRLILNLKGEPFAQDETGLFVLTDWQ
                     FSNELDFRILMDLRAAARFLAEFHLAGQGFEPVKTLPERTCWYGWPMKFIRRLRQLEE
                     FEQLAQGEAEHSEFSRLYLRHYNTFYRQAVYSLELLFRAPYSIVAQNDSTRRSLCHHD
                     FSSRNLLRTYDHRLFLVDFDYCLRDLRIHDIINLLVRNLKHNGWRVEIGRFILQEYQQ
                     VSPLSREEIEVMQVLLTWPQDFWQVGLQYYLEKLPWPKERFLKKLEEKVNTHPAREAF
                     LRRFPNENGLFRINGV"
     gene            complement(5905..6948)
                     /locus_tag="VF531_02250"
     CDS             complement(5905..6948)
                     /locus_tag="VF531_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HEX7712817.1"
                     /translation="MALENPALSSIMDHWGLHCRNWKKVKDVYKIQTESGCKNLKVSP
                     HAPERLAFVHQAVTHLNLNGFKRLNPIIPTLDGRTYVTDGTVAYTLFDWVEGGQCDFR
                     NLSVLAEATKVLAEFHQKSRGFTPPDHSNMRNRLGRCLNHFEEYYQNLIDFTEQAREM
                     PRDPFARVYLKNVDSFLPLAARATELLRQSPYQELTRAAQESMVFCHGDPAARNFILT
                     PGQGIFMIDFDSCRLDLPLMDLIKFSRRVLKKYHWDFRIARIIIEAYHEVSPLTSAEL
                     EVMRAVFYFPQKFWRISIRYFHDHRHHTPEREFRKFQKYLSNKTAIYRFHKDFDAYFS
                     DSVRDGTADVLKR"
     gene            complement(7028..8020)
                     /locus_tag="VF531_02255"
     CDS             complement(7028..8020)
                     /locus_tag="VF531_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7712818.1"
                     /translation="MEPTRFLAEINHHYAISGRTIKPYGPVWRIESPGSAYALKPMHP
                     SADELRRLAAVLINLTVSGFDGLEPPLVTITGRPFMEYTGRRYMLNFWYEGSHPSFCI
                     PEQLMEIGALYARFHQAAQAIGGLDDWPLADVLGQFSGRLRFLETLFYPTNPNRIDRA
                     IRRWLPYFSEQAKRSCAGLKNLDFSGWLSHSERKGFCHNDPAPRNIIMTNGRWRLIDF
                     ELSGYACFIAEYATLLTRSLAVLGWNEEAVNTASKLLETYSRIRPLSQMEIQALPYLC
                     CFPQRFWRLCNQRYEEHVDWTEQHFARRLREIILLEPKRRTLLERLFPELRTTL"
     gene            8241..8408
                     /locus_tag="VF531_02260"
     CDS             8241..8408
                     /locus_tag="VF531_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712819.1"
                     /translation="MKNPFRHFRNIHQKILLGVAGLMLLKTLIAPNPIDVLVLIGLVL
                     VIIGCFSDDIF"
     gene            8395..9465
                     /locus_tag="VF531_02265"
     CDS             8395..9465
                     /locus_tag="VF531_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7712820.1"
                     /translation="MISFSRSGLALPLEVPDQGVRVAVGPVSEGSPEQILAGWGLSGS
                     IRPYRRVYLMELPGECEFSRFWILKPMRIGAGRAACIARLLSHGAEWGIIPDLRWCGS
                     DLLHRGLYQWYRGNRYLISRFLPGRTAEYQDYHDLSAALQTMGSFHVLTTRLLGEEPQ
                     HWEILRYNPTREWRIRLGELRNCKQLAYRMGDDWSRRYLKVWDGFYDQAQVALEELEQ
                     CYETASSFPTLCYHDWAHHNVLIDSKNAYLIDFDELILDCPAHDRANLIGRYLRLRDW
                     SIKSLHRIIFENNRIYPWRENELRLTRIYLTFPYDYWILGRQYLIEQQPWSHRYYEDQ
                     WQRKIAPNHRRLKLLEIMARME"
     gene            9507..10436
                     /locus_tag="VF531_02270"
     CDS             9507..10436
                     /locus_tag="VF531_02270"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX7712821.1"
                     /translation="MFDQVLIRSGAAEAERWVAADLHIHSVYSGGCLTPAEIIGEAQQ
                     RLLEVAAIADHFQVKGALEGKQLAQAVPGNPEILSAQEISLGDHFHLVVIGVTEPWPE
                     GHRTQLMTRLVDHHRQGGIIILAHPWTIPRNGWGPGCLRDMLGGGVLDAAELFNASIL
                     SQGPRSNYLLQSVWEELIVPHQLAVVGGSDFHRFNQGRFIGGGRTYLYVKTPGVQGIM
                     EALRERRTVAGLFSNRPFNLGTFGAGNSVIFGVEPWYGKLKEVIADLQCRLTRVYTEG
                     YGQLDPSLIRFWSSLLATGQYQHLQEFLTVGII"
     gene            10686..11117
                     /gene="mraZ"
                     /locus_tag="VF531_02275"
     CDS             10686..11117
                     /gene="mraZ"
                     /locus_tag="VF531_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7712822.1"
                     /translation="MLMGEFQHSLDEKGRIIIPAKFREELGDKFIITRGLDNCLFVYP
                     VTQWRILEDKIKELPTSQPETRAFVRLFFSGAVEAELDKQGRVILPQTLREHARIEKD
                     VYVIGVSTKVEIWAKETWENYSAQTQKSYEEIAQNIISIGI"
     gene            11228..12187
                     /gene="rsmH"
                     /locus_tag="VF531_02280"
     CDS             11228..12187
                     /gene="rsmH"
                     /locus_tag="VF531_02280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7712823.1"
                     /translation="MPEFHHQSVLFTEAMDFLNLKSDGVYIDATVGGAGHTLGIARVL
                     GTSGRIVGIDQDRNAIIAAKERLVNVAPRIDLFHRNYVELQAITHEAGVAAVDGILFD
                     LGVSSPQLDQEERGFSYKVDAPLDMRMDQSQPFSAAHLVNTASVEELTRILWEYGEER
                     FSKRIASFIDTHRRDHEIKTTGELAAIIKQAIPAAARRSGPHPARRSFQALRIAVNHE
                     LEYLEQALGQAIRVLKPGGRIVVISFHSLEDRVVKTTFAKWAQGCICPKNLPVCICHR
                     HPEIRILTKKPLVPTEAEIVANPRSRSSKLRAAERLEPGTAVD"
     gene            12312..12836
                     /locus_tag="VF531_02285"
     CDS             12312..12836
                     /locus_tag="VF531_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712824.1"
                     /translation="MLLAERRSMINPAEMEPVPETVSGLNTSEWVARDTTVKPRKSLP
                     LLIKLTTGLLCLIVANLVIQSLVIQRISEIKHWQNKIGVLERSASELRVEMADLESFD
                     RIQSLAEKDLGMRVAGPNDYHCIAAVSDSQPVQPRDITRSSGRPSKSDRNGNLWARVS
                     TWIGEFGEAMAQTP"
     gene            12884..15082
                     /locus_tag="VF531_02290"
     CDS             12884..15082
                     /locus_tag="VF531_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7712825.1"
                     /translation="MSQIETISKKRIALLLFLIFFIITGLILRMIYLQFFMAGWLKKA
                     AEGQRFRALPLLPHRGTIYDRVGNELAISIDGDSVYAIPAEVRYEKKKEARGRVWAKV
                     DGTQNGLDEKKAIAATVADILGMDQQEVERLLSKRASFVWLKRKASFEEIDKLRRAVN
                     TEGISGIEFTQKARRFYPQSFLASQVLGIAGIDNQGLEGIEKQYDSYLQGIPGSDQAE
                     FDTAGRHIPQGERRYLEPVDGDSLYLTIDENIQYIVERELEKAVTDTKSKRGMALVVD
                     PKTGEILALACRPKFDPNRFGDYPASYRRNPIFTDMYEPGSTFKIFTTIAALEEGKVS
                     LDSTFFDPGYIIVDGQPLKCWKPGGHGSETFVEALENSCNPVFASLALRVTKETFYKY
                     IKAFGFGRMTGIDFPGETPGYLKPLTKVKNVELANIGFGQGISVTPIQMVMGVAAIAN
                     GGYLLKPQLVKKIIGPDGKVKMESGRRVVSQAISAKTATQMSKLLQSVVTNGSGNQAY
                     LEGYRVAGKTGTAQKVVPGARGYSQNIASFVGFAPADNPRILGMVILDEPGGPVTYGG
                     VIAAPVLGNIFRDTLRYLNVQPRFEPEQLEKLSSDEILVPNILNMPIAEAEQLLRSRQ
                     IDYRLMGRGSYVFDQVPKPGARIKKDSKILIYFDPEEHYNYNNSGKVVLPNFQGYTLR
                     KVDQILTEMGLVLEARGMGVAVAQNPAPGTAVEYGSTVRIDFKPVKDNLP"
     gene            15254..16726
                     /locus_tag="VF531_02295"
     CDS             15254..16726
                     /locus_tag="VF531_02295"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7712826.1"
                     /translation="MQLAELINTLTPRKVTGNLNQEITGLAQDSRRVQAGNLFFCIKG
                     VKYDGHQFLKEVATRGGTAAIVSDPPAETYGLTIIQVDKVSSQLVKEVAGAFYGYPDR
                     KLQLIGVTGTNGKTTSTYLVKSILESAGHKVGLIGTITNLIKDRPLATHNTTPGVLEL
                     QELFAEMVRSGVEYVVMEVSSHSIDQGRVTGLDFRCGIFTNITQDHLDYHKTFAEYLR
                     VKSKFFLDLPTTSWAIINGDDPHSESIIEKTAAKVVTYGVEHSAQISATNIQLSPTGV
                     SYTANTPVGPLELRLHLTGYFNVYNSLGALGAGLVLGMDPAAIKQGLEAVPGVPGRFQ
                     VVPGVTDFGVIVDYAHTPDGLENILNTGRALTKKRLLLVFGCGGDRDRTKRPIMGALA
                     ANLADYSIITSDNPRSEQPEAIIAEIEAGFKAANPGAAYQLEPDRAAAIRKIIKLAQP
                     GDLVIIAGKGHENYQIFKDRTIHFDDREVAQAALEELQHG"
     gene            16719..18134
                     /gene="murF"
                     /locus_tag="VF531_02300"
     CDS             16719..18134
                     /gene="murF"
                     /locus_tag="VF531_02300"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX7712827.1"
                     /translation="MADFTLEELAAATKGRLLRGEPGKTVHGLTIDSRRIEPGQLFLA
                     LKGERFDGHDFISAALAGGARAVVLDDEGRFDEEQFRVYPDPGVILVPDSQEALGAIA
                     RFHRDRFQIPVIAVTGSNGKTTTKDLVAAILAQEWPIIKTEANFNNEIGLPLTLLKID
                     QTTRAAVVEMGMRGLGQIRHLCGIARPEMGIVTNVGLSHLELLGSQENIARAKAELIE
                     ALPGTGLAVLNGDDPYVRAMTRKRQSDGGLRTVRYGIDGPELDYRADKYRVIENGSSF
                     RLVYPKGSFEVTLPIPGRHNVLNALAAIALAKELGFSDTAIRKGLANPGLTEKRLNRI
                     ECRGFTIIDDTYNASPASVRAAIDVLCSLPNRNRRFAVLSDMLELGTTAASSHREIGT
                     YAAERGIDHLFAWGDLALEYTSGMNDVNPGKAAFFTSKSALVAELKSCLKPGDAVLVK
                     GSRSMKMEEVVKALSGEGTEG"
     gene            18134..19114
                     /gene="mraY"
                     /locus_tag="VF531_02305"
     CDS             18134..19114
                     /gene="mraY"
                     /locus_tag="VF531_02305"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7712828.1"
                     /translation="MPEIVYVLLGALLSFATALAIGPFAIKLLHRLKFGQSIRSEGPQ
                     SHLKKAGTPTMGGVIILVALLVGILVVGKLTTEVVFLLLLTVGFGIIGLIDDLIIVIT
                     KRNLGLTARQKLFAQIVLATVAAVFLSRVPSATTQLIPFTNLKLTFPVLSFNPLFLIF
                     AAFVIVSESNAVNLTDGLDGLAGGTVAIAVLTFGALALVMGEYGVALFSFCLAGACFG
                     FVWFNCPPAKVFMGDTGSLALGAALAGIALYTRLSFFLLIVGGIFLAEVLSDVIQVAS
                     YKTSGKRGFRMAPLHHHFELGGMLEPQIMSRFWMIGMVLGVLGVLGFFVK"
     gene            19338..20435
                     /gene="ftsW"
                     /locus_tag="VF531_02310"
     CDS             19338..20435
                     /gene="ftsW"
                     /locus_tag="VF531_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7712829.1"
                     /translation="MKHNPPDLVLLFVTITLLVIGLIVVTSASAPESLSHTGGSSVFY
                     YGLRQLFFAVVGIGMMLIAMKFPYRQLRKFTIPLTLVSLALLILVLFLADPRKGSTRW
                     FELGLLGLNVQPSEIAKLAVIMVLAHYISEINTGIKNFVVGVFIPLVFVSIICLLILL
                     EPDLGTTIVIFATFVMMLFAGGANLFHLGCLGLVGVGMGIVLVVTEPYRFQRLIAFWN
                     PWKDPKGDGWQIIQSLYALGSGGPLGLGLGMSRQKFNYLPEAHTDYIFSILGEELGLL
                     GTITVIILFFFMAWRGYKIAISTKEPYGRMLAVGITSYLVFQAILNIGVVTSSLPVTG
                     ITLPFLSYGGSSLMISLVGIGILLNISKNTE"
     gene            20446..21561
                     /gene="murG"
                     /locus_tag="VF531_02315"
     CDS             20446..21561
                     /gene="murG"
                     /locus_tag="VF531_02315"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7712830.1"
                     /translation="MRVIMAGGGTGGHIYPALTIAKEIMRRRPDTEILFIGGKRGLEA
                     EIIPKEGFRLITLNLEGIPRRISLKVFRSMGLAARGVWQTFQIVKEFRPDLVVGTGGY
                     VCGPVVLAAALMRIPNAIQEQNAFPGLTNRTLGRFVDRIFLAYPEAGRFFRKSKIRVC
                     GNPIRSAEFAGVVRAKAENALGLAPNHTNLFVFGGSQSARKINQTLLVIISDLLREFS
                     KLQIVMMTGLKEHQSVEQQVAKMKLSPELRNRLFLVAYFYKIADAYKASDLVLARAGA
                     ISLAEITCFGLPALLIPFPFATNNHQEFNARVLEKHGAAKVLLEAELSPENLKLALTE
                     LLQNENNRRAMARASLAMSRPQAAEDIVTELLKLAKS"
     gene            21669..22970
                     /gene="murA"
                     /locus_tag="VF531_02320"
     CDS             21669..22970
                     /gene="murA"
                     /locus_tag="VF531_02320"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7712831.1"
                     /translation="MNETGNTIQPISPEITSKLEIQGGRRLEGTVQISGAKNAVLKLM
                     PAALLANNRCLIRNVPRIRDVEIMIGVLRGLGAEVNWEDESTLMLKSSSELGFAAPDE
                     LVREMRASVQVMGPLLTKVGRVKVFQPGGDVIGQRPINLHLKGFQALGAEIVEEHGYV
                     CLEAKKLKGAEIHLDFPSVGATENIMAAAIQAEGTTVIRNAAREPEIIEEQNFYNRLG
                     ARIRGAGTDTIRIEGVSRLNDKEIDYAVIPDRIEAGTLMIAAAITGGDVTLKEVIPEH
                     LEALLAKLREIGVEISCEEENVRVKASRKFKAMDVTVLPYPGFPTDLQPQITALLTIA
                     DGTSIITENVYSSRFRYVDELIRMGAAITVESRSAIVRGVNALSGALVLAPDIRAAAA
                     LVIAGLAAEGRTVIEGLLHLDRGYEKIEVKLSTLGASIQRV"
     gene            23477..24781
                     /gene="murA"
                     /locus_tag="VF531_02325"
     CDS             23477..24781
                     /gene="murA"
                     /locus_tag="VF531_02325"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7712832.1"
                     /translation="MANLGTIEQVKTEKSMGHLKIAGGRKLEGSVRISGAKNAVLKLM
                     AASLLANNRCIIRNVPRIRDVETMIGVLRSLGVDVQWEDETVISIRTVGGLGFTAPDN
                     LVREMRASVQVMGPLLTRTGKVKLFQPGGCVIGQRPIDLHLKGLQLLGAKVVEEHGYV
                     FVEAPRLRGAEIHLDFPSVGATENIMAAAILAEGTTTIRNAAKEPEIIEEQNFYNRLG
                     ARIRGAGTDTIRIDGVARLNDREIDYAVIPDRIEAGTYIIASAITGGRITLCDVIPEH
                     IDALMAKMREIGAEFEIGEEQIKVRNVRRLQAADVTVLPYPGFPTDLQPQITALLSLA
                     EGTSVITENVFGSRFRYVDELIRMGTNIRVESRSAIVKGVHSLSGASVFAPDLRAGAA
                     LVIAGLAAEGQTVIDGLGYLDRGYEQIEIKLANLGAAITREE"
BASE COUNT         5927 a         5795 c         6855 g         6214 t
ORIGIN      
        1 tggatcggta ttaccatcat ccccaggact ttcaagttga ccccggagaa tggcaggagt
       61 tggaacaagc gttcaatcgg ggtttctcaa ccggttatgc cggaggtaac cgcaaccatg
      121 aactgatgag ctttacccgt cccaataatc gtggggttta tctgggacgg gtggctttct
      181 tcgataaact tcagcgccga ttgcggttga aacttgaggc ggacttggag atcggggatg
      241 aggtggaggc ctgggtaagt caaggcggcc gggcagttag tccggttcgg gagcttttcc
      301 gggacggtcg caaaattgat caggccccgc ggggttccgt ggttgaatta accattgacc
      361 ataaagtttt tccgggagac cggatcttca aagtgttttc aatccaaaac gaccgccgcc
      421 taaaacaggg gcttgccccg gacagcgttg cacttaaaat cccctgcaat gctgaggtaa
      481 acgggaacgc cggcggagcg ctccggatca cccttcggga tgccaccggt aatcgtggta
      541 cagccttgag tgatacgccc ctgcaacccg caaaaaaccg gcctcttacc cgggatgtgc
      601 tcgaggaaca gctgggcaga ctgggaaata caccctttcg cttgggaggc ttggagattt
      661 ccctccccga gaatgtaatg ttgcctatcg gggaattgaa tcagttacgt cgtaaggcga
      721 tacaagaact gaccgcaaaa aaactcgata tttatcgtcg cccccgggta aagctgccgg
      781 attctttaag cctcacggca ggcccagtaa agttcgatgg atctcaagcc gaaaaatctt
      841 ccgggtctgt tccggttcaa tcagaggggc gcttgctcag cgtatgggtg ggagatttgg
      901 agggggtcgc cgcggcagcc ggcgaaggtg cggcgttaat ctacgccggt ggtgatgaac
      961 tgaccggatt tcattgggac gagaaatcgt ttcgggaagc aattgatatc gcccatctat
     1021 cccgggcgga attggttgtg ggtttaccgc ggatcaatcg cgaaggtcag cgcggcatat
     1081 gggagcaatg cctggagtta gccctgggac ttccggctga tggaatcatg atttccgacc
     1141 ttggaacgct tcaaagagtc caggagtatg ggactgggcc gctctatctc aattatccgc
     1201 taaattgctt caattccgcg gccttggccg aactcgccgg cccgcggatt gcccagatta
     1261 caatctcacc ggagttgacc ctggagcaga tatccgggct caaaaccggt atccggctgg
     1321 agtgcctggt tcaagggccg cttgaattga tggtctccga atattgcccg ctccatacgg
     1381 tacttggaaa gaccggagcc tgtcccggat tgtgcagcaa ccggggacag tttgcattac
     1441 gggacaggct caacttagat tttccgatct atcccgatca gtactgccga atgcacctct
     1501 taaacagtgt ggatctttgt ctgtacgccg atctggatca tgcggctttc tccggcttgg
     1561 tctggcggtt ggaactcaag actctgcctg cacagaaagt cgcggccatt gtccggcttt
     1621 accgccaggt agcaaacgac cccgggatcg cggcccggca tggagatatt gttctggatc
     1681 agctcaagga acttaccgga cggggtatca caaaaggtca ctatttccgg ggggtggaat
     1741 gagcagagac cgggtggtag cgaatatttt tccttgacag tgttccgatt tatggtacaa
     1801 ttagtttggt taacgccatt tgtggcgtga ccgcaaccac tggctgggag tttacgcaac
     1861 tccggcgaaa ccctcggcgg caggcggtac gtgccaagca cgttgatatt caaaggaggt
     1921 gaattcaagt tgctgactgc tgataaaaag catgatctca tcagtaaata tggtttacac
     1981 gaaggagata ccggttctcc cgaggtacag atcgcgattt tgaccgaacg gatcaattac
     2041 ctcaccgaac atctgaaagt ccataaaaaa gaccaccatt cacggcgtgg tttgctgaaa
     2101 atggtcggac aacgccgcgg tttgctgaat tatctgagcg aaaaggacat cactcggtat
     2161 cgtgcaatag ttgaacggtt aggtcttcgt aaatagtcag aaaagcgggt tatccgcttt
     2221 tctttctata tggaacattt tttccggagt atggtagact gatgtttttc aagggaccgt
     2281 tcttcgacgg ttgcttgaaa aaaccggcgg ttttttcggc ggaagggaca tactaacacc
     2341 gagattatcc tgttttattg gtcaaagata gtagggtatg gtaagggggt tttacgtgat
     2401 gcagaaatgg gagatgaccc tggggggtcg tcctctggtc attgaatacg gaactgtggc
     2461 taaacaggcc aatgccgccg tgttggtccg ttttggagaa acagtagtgc tggtaactgc
     2521 ggtaatgtcg gataagatcc gtgagggcat tgacttcttt ccattattag tggattatga
     2581 agaacgcctc tatgcggtgg gtaagattcc cggcggcttc atcaagcggg aaggccggcc
     2641 tgcagagagc gcggtcttag cggcccggac catcgaccgt ccgattcggc ctctatttcc
     2701 ggaggggttt cggaatgatg tccaggttgc ggcgacggtg ctttcggtag atcccgacaa
     2761 cgaaccgagt ttggcggctt taatcggagc ttcggcggcg ttacatattt ccgagattcc
     2821 ttttgccgga cccatcggcg gagtgaaggt cggccgggtg aatggtgagt atattatcga
     2881 tccgaccgta gcccagcaag aaaagtccga tctgcaactg gtagtggccg gtacgaagga
     2941 tgccatcatg atggttgaag ccggcgccaa cgagattagc gaggatgatg cgctcgaggc
     3001 aatcctttac ggtcattcgg tgattcggga gatcgtcgcc tttatcgagc ggatccgggc
     3061 tgatgtcggt aagcccaaga ttgaaccggt attggacgag ccaatgccgg atatcgatcg
     3121 aatggttcgc gattttgcca ctgcgaaaat ggccgtggct attaaaaacg gcgataaact
     3181 ggaacgtgaa gcccatattg aagaggttaa agaagaggcg cgtcagtatt ttcagaccca
     3241 gatgggtgaa acgtacgccg ccaaccttaa atacatcaat atggtactgg ataatcttct
     3301 taaggaagaa gtacgcaaga tgatcgcggt agagaagatc cggcctgacg gccgtaaact
     3361 ctccgagatc cggccgatca gttgcgaggt ggggttgctc cccagagctc atggttccgg
     3421 gttgttcacc cgtggtcaga cccaggtttt aaccgtttgt acactgggac gggtgagtga
     3481 ggaacagatc ctggacggct tgggtgagga tgattccaag cggtacatac atcattataa
     3541 tttcccaggt tacagtgtgg gtgaagtacg acccgtacga agccccggac ggcgtgagat
     3601 tggtcacggc gctttggcgg aacgggcatt attgccggtc attccaaatg aagaggaatt
     3661 tccgtatacc atccgtttag tctccgaggt attggaatcg aacggctctt cctcccaggc
     3721 cagtgtctgc gggagcacgt tatccctgat ggatgcgggg gtaccgattc ggaaacccgt
     3781 agccggggta gccatgggac tgctgaaaga cggcgaggat ttcgcgatac tcaccgatat
     3841 tcagggcctt gaggatcact ttggggatat ggacttcaag gtggcaggaa ccaaggatgg
     3901 catcaccgcc attcaaatgg atattaagat taagggaatc ggcagggaga ttctcaaaca
     3961 agccttagcc caggcacgag aaggccgact ttttatcctg gataagatgt tgagcacaat
     4021 ctcacaacct cgaacagagc tgtccgcata cgcgccgcga atcatccgtt tctcgatcga
     4081 tcccgatcgg atccgcgatg tcattgggcc gggtggtaag attatccgga agatcgttga
     4141 ggataccggg gcggagattg acatcgagga tgacggcagg gtcttcattg ccgcggttga
     4201 tgcttccgcg ggtaccaaag cccaggagat catcaaacgg ctcacgagcg atgtggaaat
     4261 tggcgcggtt tatctcggta aagtaacaaa attgatgaac tttggagctt tcgtcgaggt
     4321 acttcccggt aaagaaggac tggtgcacat ctcacaactg gccaaggaac gagttgccaa
     4381 ggtggaagac gtggtcaatg tcggcgacga gatcatggtc aaggtgattg agatcgataa
     4441 gcaaggccgg atcaatctct cccgtaaggc ggtattaacc gatgagaccc cggtggcggt
     4501 tcattaattt acaattttga tcattcactc aaggaagcct tccttttgca gggaggtttt
     4561 tttatttatc gcgccccaca gggcgcgcgg caacagggaa caggcaatag gcaatagcat
     4621 gaggtttgga tgtgaagaag aataataggg gtagtagacc aaatccggat ccatctaccc
     4681 tatccctgca agggaactag cttggcgttg caagtataaa caccaaagac ttagcgaatc
     4741 ccctttaagg atggatagta gacctgcgct accccgcctt ttatctgttg cctgttgcct
     4801 agtcgctatt gcctctagtt ttttagcctc tcgtatcaca ccccattgat gcgaaacagt
     4861 ccgttttcat tcggaaacct tcgtaaaaaa gcctccctgg ccggatgggt atttaccttt
     4921 tcctcaagtt tcttgagaaa ccgttcctta ggccaaggta gtttttccag gtagtattgg
     4981 agtccaacct gccaaaaatc ctgaggccat gtgagcagga cctgcatgac ttcaatttct
     5041 tctctggaaa gcggtgagac ctgctgatat tcctgaagga taaagcggcc tatttcaact
     5101 ctccagccgt tgtgtttcag atttctgacc agcaggttga tgatgtcgtg aattctgaga
     5161 tcacgcaggc aatagtcaaa atccaccagg aacagccgat ggtcgtatgt tcggagtaaa
     5221 ttgcggctgg agaaatcatg gtggcataga ctgcggcggg tgctgtcgtt ttgggcaaca
     5281 atcgagtagg gtgcccgaaa aagcaattct aggctgtaaa cggcctggcg gtaaaatgta
     5341 ttataatgcc ggagatatag ccggctaaac tctgaatgct cagcctcgcc ctgagccagt
     5401 tgttcaaatt cttccaattg ccgcaaccgg cggatgaatt tcattggcca accgtaccag
     5461 caggtgcgtt cgggtaaggt cttcaccggt tcaaacccct gtcctgccag gtgaaactcg
     5521 gccagaaatc gtgcggcggc ccgcagatcc atcaggatcc gaaagtctaa ttcattgctg
     5581 aactgccaat ccgtcaagac aaacagtccg gtttcatcct gtgcaaaagg ttcacctttc
     5641 aggttaagaa tgagacgtgg tgcccttcgg aagccccgcc cgttaagata ctccaagact
     5701 tcgaaaatga acctaacttc ctcttggttt aatttgctgc gcttcaaata tttataaccg
     5761 atctcggttt gtacccgcca tattccgcga taggtttcgt acgataaaac tttcaggccg
     5821 aaacccgcca tgattgcggt gatggatggc attttgagtg attcgggctt aacttgacta
     5881 gagctcaatt catccatctc cattttaccg tttcaaaaca tcggcagtac catcccggac
     5941 tgaatccgaa aagtaggcgt caaaatcttt atgaaaacga tagatcgccg ttttattact
     6001 gaggtacttt tggaatttcc tgaattcacg ctccggcgtg tgatgacggt gatcatggaa
     6061 atagcggatg gagattctcc aaaatttttg cgggaaataa aagaccgctc gcatcacctc
     6121 caactcggcc gaggttaagg ggctcacctc atgataggcc tcgattataa tccgggcaat
     6181 ccgaaaatcc caatggtatt tttttaatac ccgcctacta aatttgatca aatccatcaa
     6241 tggtagatcc aaacgacagc tatcaaaatc gatcatgaag atcccctgcc ccggagtgag
     6301 gatgaagttg cgggcggccg gatctccatg acagaatacc atactctcct gggccgcgcg
     6361 ggttagttcc tgatacggac tttgtcgcaa aagctccgtg gccctggctg cgagtggtaa
     6421 aaaggaatca acatttttta ggtagacccg cgcaaagggg tctctgggca tttcccgggc
     6481 ttgctccgta aaatcaatta aattttggta atactcctcg aaatgattta gacatctccc
     6541 caaacggttt ctcatattcg aatgatccgg cggggtgaat ccccgggatt tttggtgaaa
     6601 ctcggccaat actttggtgg cttcggccag aacggataga tttcgaaaat cgcattgccc
     6661 tccttccacc caatcaaaga gggtgtatgc cacggtgccg tccgttacat aggtgcgtcc
     6721 gtccagcgtg ggaatgatcg ggtttagccg cttaaacccg tttaggttca aatgggttac
     6781 ggcttgatgg acaaaggcca ggcgttccgg ggcgtgcggc gatactttta aatttttaca
     6841 cccggattcg gtttgaattt tataaacatc cttcaccttt ttccaatttc ggcagtgcag
     6901 tccccaatga tccatgatag acgaaagtgc cgggttttcc agggccaaat gaccccctcc
     6961 tccccccttc atcggccgat ccgatccggg gtgaaattca agcgttcagc cgcgattaac
     7021 cttatcttca aagcgtagtg cggagttcgg ggaataagcg ttccagtaag gtccgacgtt
     7081 tgggttccag gagaatgatc tcccgaagcc ttcttgcaaa atgctgttcc gtccagtcca
     7141 catgttcttc ataacgttga ttgcataaac gccaaaagcg ttgcgggaaa caacagagat
     7201 agggaagagc ctggatctcc atttgcgata agggccttat ccggctatag gtttccagca
     7261 acttacttgc ggtgttgacg gcctcctcgt tccagcccag aaccgccagg ctccgggtca
     7321 ataaggtggc atattctgcg ataaaacagg catagccgct tagttcaaaa tcgatcaatc
     7381 gccatcttcc gttcgtcatt atgatattac ggggagcagg atcattgtga caaaaaccct
     7441 tacgctccga atgggatagc catcccgaga agtcaaggtt tttaaggcct gcacaacttc
     7501 gcttagcctg ttcactaaaa tagggcagcc aacgacgaat ggcccgatcg atgcgattgg
     7561 gatttgtcgg gtagaaaagc gtctccaaaa atctaaggcg tccactgaat tgccccaaga
     7621 catcggcaag cggccagtca tccaaaccac cgatggcctg ggcagcttga tggaaccttg
     7681 cgtagagcgc ccctatctcc atcaattgtt ccggaatgca aaatgatggg tgactcccct
     7741 cataccagaa gttcaacata taacgacggc ccgtatattc catgaaaggc cttccagtga
     7801 tggtaacaag cggtggttcc aagccgtcga aacctgaaac cgttagatta atcagtacgg
     7861 ctgccaaccg gcgaagttca tcggccgaag gatgcatcgg tttaagagca taggcgctac
     7921 cgggagattc gatacgccaa accggtccgt atggtttgat ggttctcccg gagatagcgt
     7981 aatggtgatt aatctcagcc aagaaccggg ttggctccaa ttgccggcct ccttcgaaga
     8041 atgggatcgg gcaagattga aatagagctt tatatactat gtatgccttc tggcacgggg
     8101 aacaccttgt cacaaaaccg gtgggggaaa gagttttgcg gccaaaggtt aaaacgggtt
     8161 ttttagagtg atccggttcc ctccgggtcc cgggcgaata ccatagtaac gtagatgggc
     8221 gaatgacgga ggtggccggg gtgaaaaatc cgttccgaca ttttagaaat attcaccaga
     8281 agatcttgtt gggggtcgcg ggattgatgc tgcttaagac cttgattgct cccaatccca
     8341 tcgatgtcct ggtattgatc ggtttggtcc tggtgatcat cggatgtttc agtgatgata
     8401 tcttttagcc ggagcggact tgcactgccg ctggaagtgc ccgatcaagg tgtgagagta
     8461 gccgtgggtc cggtttcgga aggcagcccg gaacaaattt tggctgggtg gggtctctcg
     8521 ggttcgatcc gtccgtatcg ccgtgtctat ctgatggagc tgccgggtga atgtgagttc
     8581 tcgcgctttt ggatcctaaa accaatgcgg atcggcgcgg gacgggcggc ctgtattgca
     8641 aggctcttaa gccacggcgc cgaatgggga atcattcccg atctgcgctg gtgcgggagc
     8701 gatctcttgc atcgcgggtt gtatcaatgg tatcggggca atcgttatct gatcagccgt
     8761 tttcttccgg gtcggactgc cgagtatcag gattaccatg acctgagtgc ggcgcttcaa
     8821 accatgggaa gttttcatgt actaacgacc aggcttttgg gggaagaacc gcaacattgg
     8881 gagatactgc gttacaatcc tacccgggaa tggcgaattc gcctcggaga gctcaggaat
     8941 tgcaaacagt tggcatatag aatgggtgat gactggagcc gccgttatct caaggtgtgg
     9001 gatgggtttt atgaccaggc ccaagtcgca cttgaggagt tggaacagtg ttacgaaacg
     9061 gcgtcttcct tcccgaccct ttgctatcac gattgggccc atcataacgt gttgattgat
     9121 tcaaaaaatg catatcttat tgattttgat gagttgatac tggattgtcc ggctcacgat
     9181 cgcgccaacc tgattggccg ttacttaagg ctccgggatt ggtccatcaa gtccctccat
     9241 cggatcattt ttgagaataa ccggatttat ccctggcggg aaaacgagtt gagattaacc
     9301 cggatatact tgacgtttcc atatgattat tggatcttgg gcagacaata tttgatagaa
     9361 caacaaccct ggtcacaccg gtattatgag gatcaatggc agcgtaagat tgctcccaat
     9421 caccgtcgtt tgaaactcct ggaaataatg gcccggatgg agtaagggaa tgaagttgcg
     9481 cggatgcgct tgaaggttgg tgaagaatgt tcgatcaagt attgatacgg tccggggcgg
     9541 cagaggccga gcgctgggtg gctgcggatc tgcatattca cagtgtttat tcggggggat
     9601 gccttactcc ggcggaaatc atcggcgagg cgcaacagcg tctgctggag gtagcggcta
     9661 tcgctgatca ctttcaagtc aagggggctc ttgaagggaa acagttggcc caagccgttc
     9721 cgggaaaccc cgaaatattg tcggcgcagg agatttccct gggcgatcat tttcatctgg
     9781 tcgtcatcgg tgtcacggag ccttggccgg aaggccatcg tacccagctt atgacgcgtc
     9841 tggtcgacca ccatcggcag gggggaataa tcattctggc ccatccatgg acaattccaa
     9901 ggaacggttg gggaccgggg tgcctccggg atatgctcgg agggggggta ttggatgcgg
     9961 cggagttgtt taatgccagt atattgagtc aaggcccccg gagtaattat ttactccagt
    10021 cggtctggga ggagttaatc gttccccatc aattggcagt ggtcggaggc tcggactttc
    10081 atcgttttaa ccaagggcgc tttatcggcg gaggccgaac ctatttgtac gtgaagacgc
    10141 cgggggttca agggattatg gaggccttac gcgaacggcg aacggtggcc gggttattca
    10201 gcaaccgccc ctttaacctg ggaacctttg gagccggtaa cagcgtgatc tttggtgtcg
    10261 aaccctggta tggcaagctg aaagaggtta ttgccgattt acagtgccgc ctcacgcggg
    10321 tgtacacaga gggttacggt caactggatc caagtttgat tcggttttgg tcctcgctac
    10381 ttgctacagg tcagtatcaa catcttcaag agtttttaac ggtgggaatc atttgacttt
    10441 gggaattgca ttttaacaaa aatttaacat ttttcatgtc taccatatct gggggcttta
    10501 gggcaaagaa gcgcgtttgg ccaacaagat attgaggtcg aatacagttt cccgggttcc
    10561 tctggattat tttgcccgga aatccaggca aaatggttat caaaaaaagg aatttggtcg
    10621 ataatggaga atacaagatg ctaagtggta gctagtggag taaagtggtt ggaggttgtc
    10681 gcccgatgtt aatgggagaa ttccagcact ccctggatga aaagggtcgt atcatcatcc
    10741 ccgccaaatt tcgggaagag ctcggcgaca agttcattat tacccggggg ctggataatt
    10801 gtcttttcgt ttatccggtt acccagtggc ggattctgga ggataagata aaggagctac
    10861 ccacctccca accggaaacc cgtgcttttg tacggctctt tttttccggt gcggtggagg
    10921 ccgagttgga caagcagggc cgggttatac tccctcaaac tttacgggaa cacgcccgga
    10981 tcgaaaaaga cgtatacgtc atcggcgtct ccacgaaagt cgagatatgg gcgaaggaga
    11041 cttgggaaaa ctattccgcg caaacccaga aatcttacga ggagattgcg cagaatatca
    11101 tcagtatcgg aatttaatct tacgtacgcg aaatattggg aaaggtcccg agagccatgg
    11161 ctgcaagcct tgcggtccaa gcgatgcgct acaggtatcg tgatcttttc cggtcgaggt
    11221 gttgtcagtg cccgaattcc atcatcaatc ggtactgttc actgaggcca tggatttcct
    11281 aaatctgaag tccgatggtg tctatattga cgccacggtt ggcggggccg gtcatactct
    11341 gggaatcgcc cgggttttag ggacaagcgg aagaattgta gggattgatc aggatcggaa
    11401 cgccatcatc gcagccaagg aacggttagt caacgtggca ccccgcattg atctattcca
    11461 tcgcaattat gttgagttac aagccattac ccatgaggcg ggagtggcgg cggttgacgg
    11521 gatcctcttt gatctgggag tttcctctcc ccaattggat caggaagaac ggggatttag
    11581 ttacaaggta gatgcaccac tcgatatgcg catggatcaa tcccaaccgt tttcagccgc
    11641 ccacctggtg aataccgcct cggttgaaga attaacccgg attctctggg agtatggcga
    11701 agaacgtttt agcaagcgga ttgccagctt tatcgataca caccgccgcg atcacgagat
    11761 taaaaccacc ggtgaacttg cagccatcat caagcaagcc ataccggctg ccgcgcggcg
    11821 ttcaggtccc catcctgccc ggcgaagttt ccaggcatta cggattgcgg ttaatcatga
    11881 gctggaatat cttgagcagg cactcggtca agcgatccgg gttttaaaac cgggaggccg
    11941 gattgtagtc atctcctttc attctttgga ggatcgggtt gttaagacta cctttgccaa
    12001 gtgggcccag ggctgtatct gtcccaaaaa cctaccggtc tgtatatgcc accgccatcc
    12061 ggaaatacgg attttgacca aaaagcccct tgtcccgaca gaagcagaga tcgttgccaa
    12121 tcctcgctcc cgcagctcta aactgagggc ggccgaacga ttggagcccg gtaccgcagt
    12181 cgattaaggt cgggccgcgg acctaagggg tagtttgacc caccctatta gagcacctct
    12241 gaggattttt tgaatgcagt ttattttaca tgacgctcta ctaggcatta gattagaaag
    12301 gatgcataca catgttattg gcagagcgac gttcaatgat taatccagca gaaatggaac
    12361 ctgttccgga aacggtttcg ggtctgaata cttcggaatg ggtggctcgg gatactacgg
    12421 taaagccccg gaaatcactg ccgctcctga tcaaacttac aactggtttg ctctgcctga
    12481 tcgtcgctaa tttagtcatc caatcccttg tgattcaaag gatctccgag atcaagcact
    12541 ggcagaataa gatcggtgtt ttggaacgca gcgccagtga attacgggtg gaaatggcgg
    12601 acctggaatc tttcgaccga attcagtctt tggccgagaa agatttgggg atgcgagtcg
    12661 ccgggcctaa tgattaccat tgtatcgctg ctgtttcgga ttcccaacca gtacagccac
    12721 gggatatcac ccggtcaagc ggccgtccgt cgaaatcgga tagaaacggt aatctctggg
    12781 cgcgtgtgag tacttggatc ggggagtttg gagaggcaat ggcgcaaacc ccctgaagtt
    12841 atgcatggat ctgtgcacgg cttagtcctg gagggaataa gctttgagtc aaatagagac
    12901 aatctccaaa aaaaggattg ccttgcttct ttttttaata ttcttcatca tcaccggcct
    12961 cattttacgc atgatttact tgcagttctt tatggcgggt tggttgaaga aagccgcgga
    13021 aggccagcgt tttagggctt tgccgctcct acctcatcgt ggcaccatct acgaccgggt
    13081 cgggaatgaa ctggcaatca gtatcgacgg tgattcggtt tacgccatcc cggccgaggt
    13141 ccgatacgaa aagaaaaagg aagcccgggg ccgggtttgg gccaaagtcg acggtacgca
    13201 aaatgggctt gatgaaaaaa aagcaatcgc agccactgtc gccgatattc ttggcatgga
    13261 ccagcaggaa gtggagaggc tcctttcgaa acgagcctct tttgtttggc tgaaacggaa
    13321 agcgtcgttc gaggagatcg ataagttacg ccgggctgtc aataccgagg gaatctcggg
    13381 aattgaattt acccaaaaag cgcggcgctt ttatccccaa tcatttctgg cttcacaggt
    13441 gttaggcatt gccggtattg acaatcaagg acttgaagga attgaaaagc aatatgattc
    13501 ctatttacaa ggtatccccg gcagcgatca agccgagttc gatacggccg gacgccatat
    13561 tccccaaggg gaacggcgtt atttggaacc ggtggacggc gattctctct acctgaccat
    13621 cgatgagaac attcaatata tcgtggaacg ggaactggaa aaagcggtaa ccgacaccaa
    13681 gtccaaacgc ggcatggccc tggtggttga tccgaaaacc ggggagatcc tggctctagc
    13741 ctgccgtccc aaattcgatc ccaatcgttt tggcgattat cctgcatctt accggcggaa
    13801 tcctatcttt accgatatgt atgaacccgg ttccactttt aagatcttca ccaccatcgc
    13861 ggcactagag gaagggaagg tttctttgga ttccaccttc ttcgatccgg gctacatcat
    13921 tgtggacggt cagcctctca agtgttggaa acccggcgga catggtagtg aaacctttgt
    13981 agaagctctg gagaactcct gtaacccggt attcgcctct ttggcgctcc gggtgacgaa
    14041 agaaactttt tacaaatata ttaaagcatt cgggttcggc cggatgacgg ggattgattt
    14101 cccgggagag actccggggt atcttaagcc tttaaccaag gtaaaaaatg tggaattagc
    14161 caatatcgga ttcgggcaag gtatctcagt cactcccatt cagatggtga tgggggtggc
    14221 ggcaatcgcc aatggcgggt atttgctaaa acctcaattg gtcaagaaga ttataggccc
    14281 cgatggcaag gtcaaaatgg aatccgggcg ccgggtggtt agtcaggcca tctccgccaa
    14341 aacggcgacg cagatgagta agctgttaca atcggtagtc accaacggtt ccggtaatca
    14401 agcctacctt gaaggttacc gggtggcggg taagaccggt accgctcaaa aagtggttcc
    14461 gggagccagg gggtacagtc aaaatattgc ctcattcgtt ggctttgcac cggcggataa
    14521 tccgcggatc ctgggcatgg tcattttgga tgaaccgggt ggaccggtga catatggcgg
    14581 cgtgattgcc gcaccagtgc ttggaaacat tttccgagac acattgcgtt acttgaatgt
    14641 tcagccgcgg tttgaaccgg aacaactgga gaaactatcc agcgatgaga ttcttgtccc
    14701 gaacatcttg aacatgccga tcgccgaggc cgaacaactc ctccgaagca gacagatcga
    14761 ttatcgtttg atgggccggg ggagctacgt gttcgatcag gtccccaaac cgggggcgcg
    14821 gataaaaaag gacagcaaga tactcatcta ttttgatccc gaggaacatt ataattataa
    14881 taacagcggc aaggtagtgt tgccaaattt tcaaggttac acgttgcgaa aagtggatca
    14941 aattttgact gagatgggcc tggtactgga agcacggggg atgggggtcg cggtggcgca
    15001 aaatccggca ccgggaaccg cggttgagta tgggagtacg gtacggattg attttaagcc
    15061 ggtgaaggat aatttacctt aaattgctga tggatttata aaagtttccg cgactatctg
    15121 caggattgcc tggaaatcta gcgaataccg tattgatttc taaagccacc gccgcatgag
    15181 cggcggtgac cctaccgaac gaggcgaatc ctcggtgctt ttaactatgt gacaaggaat
    15241 agagggtgga attgtgcagt tagcggagtt aatcaatacg cttactcctc gaaaagtcac
    15301 gggcaatctt aatcaagaga tcaccggact ggctcaggat tcccggcggg ttcaagccgg
    15361 taatctattt ttttgcatca agggagttaa atatgacggc catcagtttt taaaagaagt
    15421 tgcgaccagg ggtgggacag cggcaatcgt atccgatcct cctgccgaaa catacggttt
    15481 gaccattatc caggttgaca aggtttcatc gcaactcgtc aaggaggtgg ccggagcttt
    15541 ttatggctat ccggatcgca agttgcagtt gattggggta accggcacca atggtaaaac
    15601 tacgtcgacc tatttagtca aaagtatttt ggagtctgcc ggacataaag tgggattaat
    15661 cgggaccatc accaacctga ttaaggatcg gcctctcgcc acccataaca ccaccccggg
    15721 agtcctcgaa ttgcaagagc tcttcgcgga gatggtaagg tccggcgtgg aatatgtggt
    15781 catggaagtc tcttcacact ccatcgatca aggccgggtg accggattgg acttccgttg
    15841 cggcatcttc accaatatca cccaggatca tctggattat cacaagacat ttgcagagta
    15901 tctccgggtc aaatcgaaat ttttcctgga tctgccgact acctcctggg cgataatcaa
    15961 cggcgatgat ccgcattccg aatcgatcat cgagaaaacc gcagccaagg tggttacgta
    16021 cggagtggag cattccgctc aaatttcggc taccaacatt caactctcac ctaccggggt
    16081 ttcctatact gcgaatactc cggttgggcc gctcgaactc cggttgcatt taaccgggta
    16141 ttttaatgta tataacagtc tcggcgcctt aggcgcgggt ctggtgctgg ggatggatcc
    16201 ggctgcgatc aagcaaggac tggaagcggt tcccggagta ccgggccgtt ttcaagttgt
    16261 gccgggagtg accgatttcg gggtgatcgt agattacgcc cacactccgg atggcttgga
    16321 gaatatatta aataccggaa gagcgctaac caagaaacgc ttattacttg tctttggttg
    16381 cggtggagat cgtgaccgaa ccaagcgtcc gatcatggga gccctcgccg caaatctggc
    16441 tgattactcc atcatcactt ccgacaaccc tcgtagcgaa caaccggagg caatcatcgc
    16501 cgagatcgag gccggattta aagcggccaa ccccggtgcc gcttaccagc tcgaacccga
    16561 ccgtgccgcg gcgatccgaa agatcatcaa acttgcccag ccgggagacc ttgtcattat
    16621 tgccggcaaa gggcatgaaa attatcagat ttttaaagac cggaccattc attttgacga
    16681 tcgggaagtg gcccaggcgg cattggagga gttacagcat ggctgatttc acattggagg
    16741 aactggccgc agccacaaag ggccggttgc tccggggtga accgggtaag actgttcatg
    16801 gtttaaccat cgattcccgt cgtattgaac cgggccaatt attcctggcc ctcaaaggtg
    16861 agcgttttga cgggcacgat tttatctcgg cggctttagc cggcggagcg agagcggtgg
    16921 tacttgacga tgaagggcgc tttgatgaag agcagttccg ggtttatccc gatccgggag
    16981 taatcctggt ccccgactct caggaagccc ttggggcaat cgcccgtttc catcgcgatc
    17041 gttttcagat cccggtgatt gcagtgaccg gaagtaatgg gaagaccacc accaaagatt
    17101 tggtggctgc gattttggct caggaatggc cgattatcaa aaccgaagct aattttaaca
    17161 atgagatcgg tttacccctt actttgttga agattgatca aaccacccgg gcggccgtag
    17221 tggagatggg gatgagaggg cttggccaga ttcggcacct gtgcggcatc gcccggccgg
    17281 agatggggat tgtcaccaat gttggcctga gccatctgga acttttagga tcccaggaga
    17341 atattgcccg ggctaaggcc gaattgattg aggctcttcc cggtacggga cttgccgttt
    17401 tgaacggaga cgatccgtat gttcgcgcga tgacacgcaa gcgccaatcg gatggcggct
    17461 tacggacggt ccgctacggt attgacggtc cggaacttga ttaccgggcc gataagtacc
    17521 gcgtgattga gaacggctcc agtttccgcc tggtttaccc caaggggtcc tttgaggtga
    17581 cgttacccat ccccgggcgg cataatgtgt tgaacgcttt ggccgctatc gccctcgcaa
    17641 aagaattggg attttccgac accgcaatcc ggaagggttt ggccaatccc gggcttaccg
    17701 agaagagatt gaaccggatt gaatgccggg ggtttaccat catcgatgat acgtataacg
    17761 ccagtccggc atcggtccgg gcggctatcg acgtcttatg ttcccttccc aaccggaacc
    17821 gcaggttcgc cgtgctctcg gacatgttgg aactgggaac aactgccgct tcaagtcaca
    17881 gggagattgg gacctatgct gccgaacgcg gtatcgacca cctgtttgcc tggggtgatc
    17941 tggctttaga gtacacttcg gggatgaatg atgttaatcc cggaaaagct gcatttttta
    18001 cttcgaaatc cgcattagtc gctgagctca aatcctgtct caaaccgggc gatgcggttt
    18061 tggtcaaggg ttcccggagt atgaaaatgg aggaagtagt caaagcttta tctggagaag
    18121 ggactgaagg ctaatgccgg aaattgtcta cgttttgctt ggagcgctgc tctcttttgc
    18181 cactgctctt gcaattggac cttttgccat aaaactattg caccgtctaa aatttggtca
    18241 gagtattcgc agcgaaggcc cgcaatctca tcttaaaaaa gccgggactc caacaatggg
    18301 cggggtcata attctggtgg ctttacttgt cgggatcctg gtcgtcggaa agctcaccac
    18361 ggaagtcgtc tttttgctgt tactgacggt gggattcggg attatcggtt taatcgatga
    18421 tctaatcatt gtaattacca aacgcaactt ggggctgact gcccgtcaaa aattgtttgc
    18481 ccagatcgta ctggccacgg tggctgcggt attcttatca agagttcctt cggcaacaac
    18541 gcaactgatc ccatttacca atttgaagtt gacctttccg gtcttgagtt ttaacccgct
    18601 ttttttgata ttcgccgctt tcgtcattgt ttccgaatcg aatgcggtaa accttaccga
    18661 cgggttggac ggtctcgccg gagggacagt ggccattgct gtgctgacct ttggtgcact
    18721 ggcattggtg atgggcgaat acggagtggc gttgttttca ttttgtttag ccggggcttg
    18781 cttcggtttt gtgtggttta actgtcctcc ggccaaggtt ttcatgggag ataccggttc
    18841 attggcattg ggagcggctt tggcggggat tgctctttac acccgtttga gcttctttct
    18901 tttaattgtc ggaggtatct ttttggccga ggtgctgtcg gatgtgattc aggtcgcctc
    18961 gtataaaacc tcgggaaagc ggggatttcg gatggctccg ttgcatcacc attttgaatt
    19021 gggtggtatg cttgagccgc agatcatgtc gcgtttttgg atgatcggaa tggtccttgg
    19081 ggtgttggga gtactggggt tttttgtaaa ataggcacta ggcaacagtc aacagataac
    19141 agctttccga cctgctattt gttacctttg actgtaacct gggatttgag attttattat
    19201 tggactttta ttatagatta ggtaccctaa actgttaaga ataaaggact tcttttacgc
    19261 tattgcctgt tgcctgttat ctgttgccgc gcgccccgca gggcgcgatg cagaaatttg
    19321 ttgtggaggt ttttcctttg aaacataatc ctcccgacct ggttttgctc ttcgtaacca
    19381 tcacgctgct ggtgattggc ttgatcgttg tgactagcgc tagcgccccc gagtccctga
    19441 gccataccgg gggtagtagc gttttttatt acggtttacg ccaattgttc ttcgcggttg
    19501 tgggtatcgg aatgatgctg atagcaatga aatttcccta tcggcaacta aggaagttca
    19561 ccattccgct gactttggtc agtttagcac tcctgatttt ggtgctgttt cttgccgatc
    19621 cacgcaaggg ttcgacccgt tggtttgaat tgggtctttt gggcttaaac gtccagcctt
    19681 cggaaatagc caaactggcg gtaattatgg ttcttgccca ctacatctcg gagatcaata
    19741 ccgggatcaa aaattttgtg gtgggggttt tcatcccgct ggttttcgta tccatcatct
    19801 gtcttttaat cctgcttgag ccggatctgg gtacaacgat cgttattttt gccaccttcg
    19861 tgatgatgct tttcgccgga ggagccaacc ttttccatct tggttgcctg ggattggtgg
    19921 gggtaggcat ggggatagtg ctggttgtta ccgaacccta tcgttttcaa agattgatcg
    19981 ccttctggaa cccatggaaa gatcccaaag gagacgggtg gcaaattatt cagtctttat
    20041 atgctcttgg ctcgggcggt cctttaggtt tggggctggg aatgagccgt cagaagttta
    20101 actatctgcc ggaggcccat accgattata tattctccat tttaggcgag gaattaggat
    20161 tactgggcac aatcactgtg atcatccttt ttttctttat ggcctggcgg ggttataaga
    20221 tcgccatttc caccaaggaa ccgtacggac ggatgctcgc ggtggggatt acatcatatt
    20281 tggttttcca agcgatctta aatatcggtg tggtcacttc gtcgttacct gtaaccggta
    20341 ttactttacc tttcctgagt tacggcggat cttccctgat gatcagtctg gtcgggatcg
    20401 gtattttact taatatctca aaaaacacgg aatgaaggtt gcaaaatgcg agtcattatg
    20461 gcgggcggcg ggaccggcgg ccatatctac ccggcgttaa caatcgccaa ggagatcatg
    20521 cgacgcagac ccgacacgga aattcttttt atcggaggca aacgcggttt agaggcggaa
    20581 attattccca aggaaggttt tcggctgatc actttaaatc tggaagggat tccccgtcgg
    20641 atcagtctga aagtttttcg gtccatgggt ctggcagccc ggggagtatg gcagacgttt
    20701 cagattgtta aggaattccg gcccgatctg gtcgttggaa ccggcggtta tgtatgcggc
    20761 cctgtggttt tggctgccgc tttgatgagg attcccaacg ccatccagga gcagaacgcc
    20821 tttcccggtc tgaccaaccg gaccctgggc agatttgttg atcggatctt tttggcgtac
    20881 ccggaggcag ggaggttttt taggaaatcc aagattcggg tctgcggcaa tccgatccgt
    20941 tccgcggaat ttgcgggagt cgtccgggcc aaggcagaaa atgcattggg acttgcaccg
    21001 aatcatacca acttgttcgt cttcggcggg agtcagagtg cacggaagat taatcagacc
    21061 ctgcttgtga tcatctccga tttgcttcgt gagttttcaa agcttcaaat cgtgatgatg
    21121 accgggctga aagaacatca atccgtagaa cagcaagtgg ccaaaatgaa attgagtccg
    21181 gaattacgga atcgtttatt tttagtggcc tatttttata aaattgccga tgcatataag
    21241 gcgagcgatc tggttttggc acgtgccgga gcaatctcac tggcggagat cacatgtttt
    21301 gggctacctg cattattaat cccatttccc tttgctacca acaaccatca ggaattcaac
    21361 gccagggtcc tggaaaagca cggcgctgcc aaagtattgc ttgaagcgga gctgagcccg
    21421 gagaacttga aacttgccct gactgaattg cttcagaatg agaataatcg tagggcaatg
    21481 gcccgcgcca gcttggccat gagcaggccc caggcagcgg aggatattgt gactgaacta
    21541 ctgaaactgg ccaaatcatg acggcttttt ggggggatgc aaaggacaag cttaatatcc
    21601 atcacacgca gcgcgtgcgc accatcggcc tcatccatat aaatataaac cggtaagggg
    21661 ttttacacat gaatgaaacg ggaaacacga ttcaaccgat aagtccggag atcacttcga
    21721 aacttgaaat acaaggcggt agaagacttg aggggacagt gcagatcagc ggcgcgaaaa
    21781 acgccgtttt aaagctgatg cctgcggctt tgctcgccaa taaccgctgc ctgatacgga
    21841 atgtgccccg aatccgggat gtggagatta tgatcggggt gctcagaggt ctcggcgccg
    21901 aggtaaactg ggaagatgaa tccactttaa tgcttaaaag ttcatcagaa cttggatttg
    21961 ccgctcccga tgaattggtg cgggagatga gagcctcagt acaggtaatg gggccattat
    22021 tgactaaggt gggacgagtc aaggtttttc aaccaggcgg ggatgttatc ggccaacgcc
    22081 ctatcaactt acatctcaag ggtttccagg ctctcggggc cgaaattgtt gaggaacatg
    22141 gctatgtctg tctggaggca aaaaagctga agggcgctga gatacatttg gatttcccca
    22201 gtgtgggggc tacggagaat atcatggccg ccgcgatcca agctgagggg accactgtca
    22261 tacgaaacgc cgctcgggaa ccggagatca ttgaggagca gaatttttac aaccgtttgg
    22321 gagctcggat ccggggtgcc ggaaccgata ccatccgtat cgagggtgtc tcccggttaa
    22381 atgataagga gatcgattat gcggtcattc cggatcggat tgaggccggt acactgatga
    22441 tcgccgcagc gatcaccgga ggcgatgtaa ctctcaagga ggttatcccg gaacatttgg
    22501 aggctttgct cgccaaatta agggagattg gtgtggaaat cagctgcgag gaagagaacg
    22561 tccgggtcaa ggcctcacgc aagtttaaag caatggatgt gaccgtgctt ccctaccccg
    22621 gttttcccac tgatttacaa ccgcagatca ccgcgttgtt gacaatcgcc gatggtacca
    22681 gtataataac ggagaacgtt tacagcagcc gttttcgata tgtggatgag cttatccgaa
    22741 tgggcgctgc gatcactgtg gaaagccgta gcgccattgt acgcggagtg aatgccctta
    22801 gcggggcact ggtgctggct ccggatatcc gggctgccgc ggcgttagtc atcgcgggac
    22861 tggcggctga aggccggact gtcattgagg gcttgcttca tctggatcga ggttacgaga
    22921 agatcgaggt caagttgagt actttgggtg cttcaataca gagagtttga gttaaaacac
    22981 cagacgatac aggaagaacg gttccatatt tcccaatggg ttttctgaaa gccggttgaa
    23041 tcgtctttca gagggttaaa cgcgtccgcg cgtacgagga gacaagccga cggtttcggt
    23101 tttttcacga atgcaaccgg tttttcgcct cttttttatt tgtttgtgaa tgttcaatac
    23161 tctttttgtt acggtccgtg aggacctata tttttgggct gatagtaaca gtgatatgat
    23221 tttgagatat gaaccgaaat ctatattata accaaatgta ttgccatgtg ttgcgtaagt
    23281 ataaactcgc aagcctatgt atggcggttg catgattttc ggctgacttt acttgttaac
    23341 gaagcgtcac caatttttta ccctgctgta gcgaatttga tttattctta aatcggactg
    23401 ggtacaagtt accgccgatt tactggacga atatgatggc aatggtttgg atcacgaata
    23461 gggggcagtt ttttgaatgg ctaatctggg aacaatcgaa caggttaaaa ccgaaaaatc
    23521 tatggggcat cttaagattg caggtggtag aaaactagaa ggatcggtac ggattagcgg
    23581 cgccaagaat gcggttttaa agctgatggc cgcgtcgtta cttgccaata atcgctgcat
    23641 tatacggaat gtgccccgaa tccgtgatgt ggagaccatg atcggagtgt taaggagcct
    23701 gggtgtcgat gtgcaatggg aggatgagac ggttatctcc atccgtactg tcggcggtct
    23761 cggttttaca gctcccgata atctggttcg ggagatgcgt gcttcagtac aagtgatggg
    23821 gccgcttttg accaggaccg gtaaagttaa gctttttcaa ccggggggct gtgtcatcgg
    23881 ccaacggccg atcgatctcc atttgaaagg tttgcaatta cttggagcaa aagtggtcga
    23941 ggaacacggt tatgtctttg tcgaagctcc cagactccgt ggtgccgaga tccatctgga
    24001 cttccccagc gtgggggcta ccgaaaatat tatggcagcg gccatcctgg ccgaaggaac
    24061 caccaccatc cggaatgcgg cgaaagaacc ggagatcatt gaggaacaaa acttttataa
    24121 tcgtttaggg gcacggatta gaggggctgg aaccgatacc atccggatcg acggtgtcgc
    24181 ccggttgaac gaccgggaga tcgactatgc ggttattccg gaccggatcg aggccggcac
    24241 ttacatcatc gcctcggcaa tcaccggagg ccgaattacg ctctgcgatg tgattcctga
    24301 acacatcgac gccttaatgg ccaagatgcg ggagatcgga gcggaatttg agatcggcga
    24361 ggaacagatc aaagttcgaa atgttcgccg tttacaagcg gccgatgtaa ccgtgcttcc
    24421 ttatcccggg tttcccaccg atctccaacc tcaaataacc gcgctccttt cattagccga
    24481 agggaccagc gtcattacgg agaacgtctt tggaagccgt tttcgttatg tggatgaatt
    24541 aatccggatg gggaccaata ttcgggtgga aagccgcagc gctattgtaa agggagttca
    24601 ttcgttgagc ggggcttcag tattcgcgcc ggatctgcgg gccggagctg cgctggtcat
    24661 tgccggactg gccgccgagg gtcaaactgt catcgatggt ttgggatatc tcgatcgggg
    24721 atatgaacag attgagatca aactggcaaa tctgggcgcg gcgattaccc gggaggaatg
    24781 attctcgcgc c
//
LOCUS       DASVMB010000073        19617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_155916,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000073 DASVMB010000000
VERSION     DASVMB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 19617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19617
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155916"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF531_02330"
     CDS             complement(<1..515)
                     /locus_tag="VF531_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEX7712833.1"
                     /translation="MDLIKVIAAELKFRPHQVENTIRMLDEGNTIPFIARYRKEATGE
                     LDEEELRAIEERLKYLRNLEARKEEVLHSIEEQGKLTPELTAAIAAATKIQEVEDIYR
                     PYKPKKRTRAMIARERGLEPLADLIWDQKTETGSAQQLAAAYINSDLGVEDTAAALSG
                     ACDIIAERVAD"
     gene            complement(1024..1434)
                     /locus_tag="VF531_02335"
     CDS             complement(1024..1434)
                     /locus_tag="VF531_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7712834.1"
                     /translation="MTNARPNPYEVTSAVEVRSDVKRWRFKPHSIGPYFLWGIVLFLG
                     IRLLIVPLAEGVYDYITKTQELNNMRLQYQETQKQIAQLEKTRDFMKTPAYIEERAHQ
                     IGLIKSNESQMVVVDSPPGTVVKTQPRKKVEIGD"
     gene            complement(1485..1718)
                     /locus_tag="VF531_02340"
     CDS             complement(1485..1718)
                     /locus_tag="VF531_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7712835.1"
                     /translation="MKIEIRGAENLSFRERQVVVLKEMGESAERIAKQLQLTPSSVAT
                     LYNRAKTKGYEVVIVLPGTALGIAGADEDEIEH"
     gene            2028..3164
                     /locus_tag="VF531_02345"
     CDS             2028..3164
                     /locus_tag="VF531_02345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7712836.1"
                     /translation="MGDIVIKPVLTANDRKKFIRLPWQFYKNDRNWVPPLLSDMKNTL
                     NPQKNALLSLGPYQYYLAYRGTEIVGRIGVGIDKHLNAEKHYESSYLTLFESVNDYEV
                     AKALFDTAIRWATEHGAEFITGPQSPSNGDDFRGLLIMGFDTPPSLMSSYNPPYYQEF
                     FERYGFTKQFDRNAYYVDIANSNIPDEFTRGVEYAKSRYHFRVESLDMKRFDEQVDAI
                     VKVAIAAWPIDWPDMVPPTPDEVRAEVAKLKPFVVPELVYIAWNEENQPIGIAVTLPD
                     YNQIFKRINGRLFPLGWFQFLRYKNKIDVARIFILFTIPEYWKKGVSAAIYLEGIQAA
                     RRLGYKWADGSSIHEFNVRMNQDAIGAGGKLYKVFRIYRKDLIA"
     gene            3284..3754
                     /locus_tag="VF531_02350"
     CDS             3284..3754
                     /locus_tag="VF531_02350"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX7712837.1"
                     /translation="MIKDKVTLGIVSGLAGSLVKNLVAFILEQLKLIDCVFCEMAGGL
                     FLGNPVYQGSRFLATLIGYLADSTLSIILGVSFVYFIEGTGNRFLVIKGVSFGAVVWF
                     LIFGGVMSFGVSPVRETHPVHILLLFLLHLIFGLSLGLTVEWWGQPTLDREKLK"
     gene            3909..5138
                     /locus_tag="VF531_02355"
     CDS             3909..5138
                     /locus_tag="VF531_02355"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX7712838.1"
                     /translation="MAKINQHYLELPGSYLFSEIARRIQKYLAEHDQAQIIRLGIGDV
                     TQPLAPSVIDAFQNGVAQMGQAATFHGYGPEQGYSFLRQQIIAHDYRPLGIELSEDEV
                     FISDGAKCDTGNIQEIFGLDNIVAVTDPVYPVYVDSNVMAGRAGKLGDEGRFEGLVYL
                     PCNEANGLKPRLPQTKVDLIYLCFPNNPTGMTLTHDELKSWVDYARKNQSVILFDAAY
                     EAYIQEPGIPHSIYEIEGAREVAIEFRSFSKTAGFTGTRCAFTVVPKTVLAYGEASQG
                     YELNRFWLRRQTTKFNGVSYPVQAAAAAIYTPEGQEQIRKVINYYMTNARIIREGLQA
                     LKFKVYGGVNAPYIWLKTPSGLSSWEFFDKLLNEVQIVGTPGAGFGPSGEGFFRLTAF
                     GSRENTEKAMERMKQLS"
     gene            5241..5972
                     /locus_tag="VF531_02360"
     CDS             5241..5972
                     /locus_tag="VF531_02360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7712839.1"
                     /translation="MHLKKIGRPIMIVILIGLIGLGIGLSFNRYFLIQTTYDFVNQSD
                     VLWMGFNSQKVVAFTFDDGPDPSYTPQILATLKKYRVPATFFIEGANVLKYPGLVQAE
                     LKDGHTIGNHTFDHPHLAKESVEIIREELERTDRAIREVTGQTPRIFRPPYEELTEAI
                     ITASRGLHKQLIMSTITLEHHSVKTPQTKAERVVRLVFPGAIILMHDGRLNRASTVQA
                     LPYLIRGLKSRGYRIIPLNKLVGTN"
     gene            complement(5977..6270)
                     /locus_tag="VF531_02365"
     CDS             complement(5977..6270)
                     /locus_tag="VF531_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX7712840.1"
                     /translation="MAGVNEQLCFATETKEEILARLRRAEGQLRGIQKMIEEDRSCQD
                     LVIQLVAVKSAIAQIAVTVLSNQLVHCLATDIQDGKSVEMTTDNFVEIFKKLI"
     gene            complement(6469..6780)
                     /locus_tag="VF531_02370"
     CDS             complement(6469..6780)
                     /locus_tag="VF531_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX7712841.1"
                     /translation="MSSDKTLVCKECNQEFTFTASEQDFYAEKGFTNEPSRCPSCRQA
                     RKNNGRGQSGRGGSGFRSERVLYDAVCTRCGAQTQVPFQPKPDREVLCRDCFRSKSNY
                     R"
     gene            complement(7183..7755)
                     /locus_tag="VF531_02375"
     CDS             complement(7183..7755)
                     /locus_tag="VF531_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX7712842.1"
                     /translation="MWHYEKKLQYPAKVGKADLKMAKYLITQYGGPDGELAAALRYLN
                     QRYSMPTNQTKAVLNDIGTEELAHLEIIATLVHQLTRCATAEQMRAVDLGGHYADHDH
                     ALFYVDATGNPWTATYIQAKGDPVADLHEDMAAEQKARATYQWLIDLTDDPDLKDTLN
                     FLRVREIVHFQRFGETLDIVQESQSCKHLF"
     gene            complement(7811..8071)
                     /locus_tag="VF531_02380"
     CDS             complement(7811..8071)
                     /locus_tag="VF531_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HEX7712843.1"
                     /translation="MQREQLELLKTLQALEFTALDLSLYLNTHPGDQRALTDYSNVMC
                     EVNRVRQLYTMNYGPLTAEDSVNQPVWRWAQDPWPWDLDYRE"
     gene            complement(8073..8234)
                     /locus_tag="VF531_02385"
     CDS             complement(8073..8234)
                     /locus_tag="VF531_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HEX7712844.1"
                     /translation="MDGMNRVTLELAQAYVPVQIFVNRWEPKKALMMGTIFPELYRPY
                     VSRDRERRG"
     gene            complement(8429..8665)
                     /locus_tag="VF531_02390"
     CDS             complement(8429..8665)
                     /locus_tag="VF531_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712845.1"
                     /translation="MSQCVLEPGKICNGCGSCNICDLDETKVCDNCCRCLEEADYRAI
                     SIDKILMPEPVRMKWKRGAVPKNRQAATKPGHSR"
     gene            9402..9707
                     /locus_tag="VF531_02395"
     CDS             9402..9707
                     /locus_tag="VF531_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712846.1"
                     /translation="MLYAASVKVCCGKNHRRIDVIVEADDPEKAKAKVVKQARKIYAP
                     GKKATYEIIGMVSEAEAFEKFFARMKETTEAEGPDAGSAPDEPSPNSELDLPGPRME"
     gene            complement(9718..10017)
                     /locus_tag="VF531_02400"
     CDS             complement(9718..10017)
                     /locus_tag="VF531_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX7712847.1"
                     /translation="MADLGNPFVGNIPRQISREELAQALRADLAGELEAIIGYDAHVM
                     ATTDERVKRVLSSIRDEEKQHVGELLSIIQILDPKESEFLNKGNQEVNQILGSRV"
     gene            10266..10466
                     /locus_tag="VF531_02405"
     CDS             10266..10466
                     /locus_tag="VF531_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712848.1"
                     /translation="MDKKNQIEAIVDFVGKHPQTVASRRICREVLGEGMERSNQEFSS
                     ELTSKLGSRDEEQISSYYYLIR"
     gene            complement(10526..11125)
                     /locus_tag="VF531_02410"
     CDS             complement(10526..11125)
                     /locus_tag="VF531_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7712849.1"
                     /translation="MEFRYDCPHLGPSLGTTQFYGDPDLIFLREDLADELGAIVAYQE
                     CAAEIRDRRISEQFREIAKEEAGHFIGLFRMVGSLDPVQAEELKQQGLVMLAGYAEPT
                     IAMGRPCAGCGNTSGDYREAKKNTAGEHRRFESDLRDLDCLRKAIRDELHAINAYQRQ
                     IQATNNQSIRQLLTAIMNKEKEHAAEFTKIFHEMQHKGE"
     gene            11326..13875
                     /locus_tag="VF531_02415"
     CDS             11326..13875
                     /locus_tag="VF531_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HEX7712850.1"
                     /translation="MFIFKRVYGYAYGKQRRTRALLPLILFAALLLGQMAQAASGKLI
                     DLDFKNSDLKDILRALASQAGVTFVIDNDVTGTVTLHLSKVTFGQALEILAKNNQLTY
                     TVDNKVYHISQIDTSVLKVEYDAAAGLLTVEAERVKLFRLFETLSQKTGVNLVPAPEL
                     QDRIDIQLERAPLTDGIQAILTKANCIGETIGKVTYVRKKATELYPFTINYQNNLITI
                     DAQNIPVSAVTRAITEKTGVAVVADPGVNSNVSIYIQNLPLAEALAILSDSNGLELTK
                     LDNSWRISRKAGSFRVRFKDNLLSVDADNVDVTALTTEISRQTGVNIILAREIRGTAS
                     GHFQNVPLSQALNALLEPQGWVVERQYGSYYIKPNTGQNPNLQISYDPEKQLFDITVT
                     QASPLTTILGEIARKADLNIVIMPQVNWNVNNLRLKGMNLSQTLGFLLQGTAYSCTVI
                     DQTYIIADSLYPRPENSDFQTVKVYPVRYLKADQILSTLPAIFPRQNIVPLQDKNALV
                     VTGTTELHSKFSEYLAQMDVASIEDQTEVIRIKYLKAEDILKYLPSSISKTDIIVIKE
                     MNAVSVTGPQNLINQVKQYIDKIDQVNPMIVFDILVVSISASDTVTWDPGTASILTGT
                     GGVSLTPGTGKIVTGVMDSSSILSSINLLISQNKAKVLQNPTIYTLNGYPTNFNVSKK
                     VSYNIVENIGTGTSTTPTSRQVNFDSGLFITMTPWVSANGQITLEIKPTFSTFDPGGL
                     TGTAATTGIIGTTNERTVAATVRMQDQETLVLGGLKSTNREKIISKIPILGDIPLLGF
                     LFRTTTYRDVQDEFVILATPRLIYDEADKKAAQEKFEAALGPDTQNTLSNK"
     gene            complement(13889..14347)
                     /locus_tag="VF531_02420"
     CDS             complement(13889..14347)
                     /locus_tag="VF531_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712851.1"
                     /translation="MKQIRIALATKYWLGIFAVSGGIFVWGLTSWIVARFYQPSMPVQ
                     TQVGKYSYRLPETDHQGRTDSLPGDRLFFGGAVSQNPVPAETGISFHSSLILWGTIAG
                     ETAVVGLDPNSNTATQLVRVGEVFAGEKIITIGSNYIVVKNQTGTGRVNL"
     gene            complement(14388..14891)
                     /locus_tag="VF531_02425"
     CDS             complement(14388..14891)
                     /locus_tag="VF531_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712852.1"
                     /translation="MTDREKKLLLLVGVVILAGLSFNYFSGGAKNVGVIGNALNEKEA
                     ERLLSSSGVIIARNRAATAALAKLEARFYPEISIDRAQLDLLQEVESIASRSGLAVDK
                     KSIARLPDRLIEVTLEGRTGSAALFLFMERLGETPIALKIKNLRIHSLPEERQLEYEI
                     GIISLLR"
     gene            complement(14884..16440)
                     /locus_tag="VF531_02430"
     CDS             complement(14884..16440)
                     /locus_tag="VF531_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712853.1"
                     /translation="MRTAIVIEPGPEVLAVAELRSRQSGIELCRHFVRPLPSTGLDSE
                     WLRQTWETERPGGNRVICLIPTHLVHYHTVVVPVLPDEQLEAAVRLELENGSDLHGLH
                     QIKPLYHRLRDRMYHVRAAVIPDQSLTELFELLERAGLTILWSGLRNWGNENFLNFRR
                     DFFTESAASVAFLDITPEQTELGVIQNERLVYCRSFMPGARKLRTAFEAASISPEVSE
                     SDPAQPVLNDFWEQFQLSHAAYQGLSGLTLPEPLWLLGETDEFSKPLQDSAAGAGVRL
                     RLPDEIRDVSRAASSSSGHAANDSRIHGALNRKFTSRLAPLLGLGLEVLGFYHHESRY
                     IYSARQQQIRNQKGKWRSIALAGVAACLLLIGLGLKFQAGVEKEVKIGRWLGEQSQYL
                     QRLKRTENQTGVALGRMRNLEAWLEDQGQELAWLRTLQDNLPEGTVVTDLTIEDGVVK
                     ELALTAPAASLVLKQLRQVPELAGLMLKGTIMTSNRGEVFHLQGPVAGSPKDTVTQKT
                     PVQKDKETHD"
     gene            complement(16593..17315)
                     /locus_tag="VF531_02435"
     CDS             complement(16593..17315)
                     /locus_tag="VF531_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7712854.1"
                     /translation="MGLGVPQLWWLQIIFGLVCGSFLNVVIHRLPRHESLCWPPSHCP
                     NCSHGLSWLDLVPVLSYCWLRGRCRYCGVRIKVRYPLVELLTAGLTLLWGLRYPVDVG
                     GIAILILLYTLVAIAFIDLEQMIVPDGLTIPLLLLGLAVRFWQGQWFLGLSGILAGFG
                     ILWLIRLIEPAGMGWGDIKMLAMIGAFCDWRSVIGVLFLGSFLGFAILLPFKLAQKIR
                     PRQPVPFGPFLAGAAWVVFLVI"
     gene            complement(17438..18667)
                     /locus_tag="VF531_02440"
     CDS             complement(17438..18667)
                     /locus_tag="VF531_02440"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712855.1"
                     /translation="MSRDGQEGSTLFMITCICGVLGLIATFLFYRSEVEWALVSQQQQ
                     MERCRALGLAILRSELELLAADDSPADSPQDSWYHGGRTITEQNGFQISVLIEDEGSK
                     PNLNTLDQKDLGLLTAGQSETEISFDPLLDWRDGDDQPRPSGAERDFYQGLAKPYQTR
                     NGFFSTVEELNQVKGGAALYDLLADRVTVHGLLNPNAIRPETFGELLSSVGFTPDEVV
                     TMTGSFRHYRATKDPNTGQLQRFTNIDDFLKLDGILIPTRDRLRPFFRFSGGSNLNFM
                     TREGIAVLLNQAGIGPELAAALAARISQQPFANQNEIENYFLNQNQHFISSDYFSCIS
                     TLIRYRVWISKGSVRYYLDTIQERARASDLASWTIRSLAWYERSGSSTGNDPELPQPP
                     PAEIENPPAEGESPPSE"
     gene            complement(18660..19301)
                     /locus_tag="VF531_02445"
     CDS             complement(18660..19301)
                     /locus_tag="VF531_02445"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEX7712856.1"
                     /translation="MVCPRKSHSVVIGPQTETGLTSGSSSAVPISGRGQSGFTLPEVI
                     LAISFTAILLGVLAQLLFGGMRIWERIGRGYERQHQLLLIDRWLRNDLGALITGQYLP
                     EAAIQGDQSKLEFWSRSSSGVVKVQYNFDSASGTVNRATGFWGENAVEQPVLKTVSEW
                     HFEYFDPVSENWLLSWDPQQSYRIPALIGVTVKTRDFDLGRLVFPIPGAVSHE"
     gene            complement(19252..>19617)
                     /locus_tag="VF531_02450"
     CDS             complement(19252..>19617)
                     /locus_tag="VF531_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7712857.1"
                     /translation="TGGTGLNPDGFTLFEVIVALMVLTLAISALLGSFRQLVYTEEQL
                     RGRVTALIIGRGKLAELEQGSEKGSSGVFAVPYSRFSWSCREETAADGGSRLILTLKW
                     RNQNGLSQEESFSGYRAAD"
BASE COUNT         4991 a         5250 c         4490 g         4886 t
ORIGIN      
        1 tggtccgcca ctcgctcggc gataatatcg caggctccgg acagagctgc ggccgtgtcc
       61 tccactccca aatccgaatt gatatatgcg gcggccaatt gttgggctga gcctgtttcg
      121 gttttctggt cccatatcag atcggccagg ggttctaatc ccctttccct ggcgatcatc
      181 gcccgggtgc gtttctttgg cttatagggc cgataaatgt cctccacctc ctgaatcttg
      241 gtggcggctg caattgccgc agtcaattcc ggcgtcagtt tgccttgttc ctcaatggag
      301 tgcaacactt cctctttacg ggcctccagg ttacggaggt acttgaggcg ttcttcaatc
      361 gcccttaatt cttcttcatc caattctcca gtagcctctt tacgataccg ggcaatgaac
      421 ggtatggtat tcccttcatc cagcatgcgg attgtatttt ccacttgatg tgggcggaac
      481 ttcagctcgg cggcaatcac cttgatcaga tccatggttt actccttttt aagattatgc
      541 ctcacattat atcaattcag tcccggtttc gtggaatgta atgagctcta tttcccgtta
      601 aaaatcaaca aaaattccaa cagacctcct ctttttttaa ttttcctcct tcgattggag
      661 tttttgagag aattcagcat gaaggaatag gcgcgaggca tgaggcgcga gacacgggca
      721 acaggcaaca gggaaaaaca atagacatga ggcacaagaa cgtgagccaa caggcaggca
      781 aatccctttt tagtgacagg gtaattggcc taaatcctcc tctatctacc cttccccttc
      841 cagggaacca gccgggggtc gaaggtatta acccgtaaca acttggcgca tctcctttcg
      901 gggatatggt cgtgtaaagt aaaagggtga agtttgaaaa ttattttcat tcttcaccct
      961 taatagtttc acatctattt tctattgcct gttgcctatt gcctcgcgcc cagaggggcg
     1021 cgcttaatcc cctatctcca ccttttttcg gggttgggtt tttactaccg ttcccggcgg
     1081 ggagtcgacc accaccatct gggattcgtt tgatttgatc aaccctatct gatgggcccg
     1141 ctcttcaata taggcaggag tcttcataaa atcacgggtc ttttctaact gcgcgatctg
     1201 cttttgggtc tcttgatact gcaaacgcat gttgtttaat tcctgtgtct tggtgatata
     1261 atcgtaaacc ccttccgcca gtggcacaat caataaccgg atacctaaga aaagtacgat
     1321 tccccaaaga aaatacggtc caatggaatg aggtttgaaa cgccaacgtt tcacgtcgct
     1381 gcgaacttct acggcggaag ttacttcgta agggttcggt cgtgcattgg tcatcggagc
     1441 cagattaaat caccttcttt attcgttcca atttgatatg taaattaatg ttctatttca
     1501 tcttcgtctg cgcccgcaat ccccagggct gttccgggca gaacgattac aacctcatat
     1561 cccttggttt ttgcccggtt atacaaggtc gctaccgaac tcggcgtaag ctgtaattgc
     1621 ttggcgatcc gttcggcgct ctcacccatc tcctttaaca cgaccacctg tcgttcacgg
     1681 aaactaaggt tttcagcgcc tctgatctcg attttcatta tatcaccatt actacaaaat
     1741 tactacataa tttgtacata atttcgacat ccttagacat cttcctgccg cccagatcaa
     1801 tttcatccga tcggcctggt ttgacatggc ctgttattat ggcacaagtc ggaaccaatg
     1861 aggcgcagac gcataactta aaagtggcat gccttcaact ccaaaatcct tggctggacc
     1921 ctgtgcagga tttcacggaa attgaacgaa tatatatttt ttaaatgctt ttgcggtttt
     1981 taaaaattaa cactgttcct atacgtttta agacggaggc gtgattaatg ggcgatattg
     2041 taataaaacc ggttttaact gcaaatgatc gtaaaaaatt catccgttta ccctggcagt
     2101 tttataaaaa cgaccgaaac tgggtaccgc cacttttatc cgatatgaag aacacgctca
     2161 atcctcaaaa aaatgcactc ctaagtttgg gaccctatca atattatttg gcctaccgcg
     2221 gcaccgaaat tgtcggacgg atcggagtag ggatcgacaa acatctcaat gcggagaagc
     2281 attacgagag tagttatctc acactgttcg agagtgtgaa tgattatgaa gtagccaagg
     2341 ccttatttga caccgccatt cgctgggcca cggagcacgg cgccgaattt attaccggtc
     2401 cgcaatcgcc ttcaaacggc gacgattttc ggggactgct tatcatgggg tttgatactc
     2461 ctccatcgct gatgagctca tacaatcccc cttattacca agagttcttt gaacgatacg
     2521 ggttcaccaa gcagtttgat cgcaatgcct attacgtaga tatcgccaac tcaaatattc
     2581 ccgacgagtt tacccggggg gtggaatacg ccaaaagccg gtaccatttc cgggtagaat
     2641 ccctcgatat gaaacgcttc gatgagcagg tcgatgccat cgtaaaggtg gcaatagcag
     2701 cttggccaat cgattggccg gatatggtcc caccgactcc ggatgaggtc cgggcggaag
     2761 tcgccaaact caaaccgttt gtcgttcctg aattggtcta catcgcctgg aacgaagaaa
     2821 atcaacccat cggcatcgct gtaaccctac ctgattataa ccagatcttc aaaagaatta
     2881 acggtcgtct ttttccactg ggctggttcc aattcctgcg ttataaaaat aagatcgacg
     2941 tagcccgaat ctttatccta ttcactatcc cggagtactg gaaaaaaggg gtttccgccg
     3001 ccatatacct ggaaggaatc caagcagccc gcaggttggg ttataaatgg gcagacggct
     3061 ccagtattca tgagttcaat gttagaatga accaggatgc gatcggcgcc ggcggcaaac
     3121 tttataaggt gttccgtata tatcgcaaag acttgattgc ctaaaagctt cccccaatcg
     3181 tttaggcata tccccgaaaa aactaacaaa tacttatacc gatcagcctt taagctgatc
     3241 ggtattcttt tgatcccgaa gatatggatg taggggcatt gaattgatta aggataaagt
     3301 gactttaggc attgtctcag gtttagcggg aagtctcgtc aaaaacttgg ttgctttcat
     3361 cctggagcag ctaaagttga ttgattgcgt attttgtgaa atggcgggcg gcttgttctt
     3421 gggtaacccg gtatatcaag gttcccggtt tttagcgact ttaatcggct atttggctga
     3481 tagtaccttg agcatcatcc tgggggtcag tttcgtttat tttattgaag gcactggtaa
     3541 ccgttttctt gtcatcaagg gagtcagttt tggggcagtt gtatggtttt tgatctttgg
     3601 cggcgtcatg tcattcggag tctctccggt gcgggagact caccccgttc acatactatt
     3661 actcttcctg ctgcacttga tcttcggatt gtctttagga ttgacggtcg aatggtgggg
     3721 tcaacctact ctcgaccggg agaaacttaa ataaacgttt accttacgat cgcagcccaa
     3781 ctttccggaa gttcatttgg ccgggttagc gggaaccctt cctaagcctg agcgacttgg
     3841 tgacacggca ggtttttgaa cggcagtcgc gaagatctca acaaaacttt tgaaaggatg
     3901 aacgcaagat ggccaagatc aaccagcact atctggaatt gccgggtagt tatctttttt
     3961 cggagatcgc ccggcgtatc caaaaatacc tggccgaaca cgaccaagcc cagatcatcc
     4021 gcttgggaat cggagatgtt acccaaccac tggcaccttc agtgattgac gccttccaaa
     4081 atggagtggc acagatgggt caggcagcta catttcatgg ctacggacct gaacaaggtt
     4141 actccttcct gcggcagcag atcattgccc atgattaccg gcctctggga atagaactct
     4201 ccgaagatga ggtattcatc agtgacggtg ccaaatgcga caccggaaac atccaggaga
     4261 tctttggttt ggataatatt gtcgccgtaa ctgaccctgt ttaccctgtt tatgtagaca
     4321 gcaatgtgat ggccggcagg gccggaaaat tgggggatga gggtcgcttc gagggtctcg
     4381 tttacctgcc atgtaacgag gcgaatggcc ttaaaccacg gttgccccag accaaagtcg
     4441 atctgatcta cctttgcttt cccaataatc ccaccggtat gacgctgacc catgatgaac
     4501 tgaaatcctg ggttgactac gcccgtaaaa accagtcggt aatcctcttt gacgctgctt
     4561 atgaagcgta tatccaagaa ccgggaattc ctcacagtat ttatgagatc gagggtgccc
     4621 gggaggtagc catcgaattc cgaagtttct ccaagaccgc cggatttacc ggaacccgtt
     4681 gcgcctttac ggtagtaccc aaaacagttt tggcgtacgg cgaagccagc caaggttacg
     4741 aactgaaccg tttctggctc cgccgccaaa ccaccaagtt caatggagta tcctaccctg
     4801 tccaggcggc cgctgctgca atctataccc cggaagggca agaacaaatc cggaaggtaa
     4861 tcaactatta catgacgaat gcccggatca tccgtgaagg cctccaagca ttgaagttta
     4921 aagtgtatgg cggcgtcaac gccccctata tctggctaaa aaccccgtcc gggttgagtt
     4981 cctgggaatt cttcgacaaa cttctgaatg aagttcaaat cgttggtacc ccaggggctg
     5041 gattcggccc tagcggtgaa ggattcttcc gcttaaccgc ctttggtagc cgggaaaaca
     5101 ccgaaaaagc catggaacgg atgaaacagc tctcctaaat cgatggttgc ggttttaagc
     5161 ccgcaacgaa aggatgggat cctgaccaca aatgattgcc ggggattcaa atccccggcc
     5221 tactccacag gaggtcaatc gtgcacctaa aaaaaatcgg gcggccgatc atgatcgtaa
     5281 tcctgatcgg cctgatcggg ctgggtattg gtttatcgtt taaccgttat tttctgatcc
     5341 aaaccactta cgattttgtc aatcaatcgg atgttctatg gatgggtttt aactcccaaa
     5401 aagttgtcgc ctttaccttt gatgacggac ccgatcccag ttataccccc cagatcctgg
     5461 ccacattaaa gaagtaccgt gtgccggcca ctttttttat cgaaggggca aatgttctaa
     5521 aatatcccgg attggttcag gccgaactaa aagacggcca cacaatagga aaccatactt
     5581 ttgaccatcc tcacctggcc aaggaatcgg tggaaataat ccgggaggaa ctggaaagga
     5641 ccgatcgggc gatccgagaa gtcaccggtc aaaccccgag aatattccgg cctccatatg
     5701 aggaattgac cgaagcaatc attactgcat cccggggtct tcataagcag cttattatgt
     5761 ccaccatcac gctggagcat cattccgtca aaacccctca aaccaaggcg gaacgggttg
     5821 tacggctggt cttcccgggg gcaatcattc tcatgcatga cggacggttg aaccgggcct
     5881 caaccgtaca agccctacct tacttgattc ggggtttgaa atctcggggt tatcggatca
     5941 tcccgctcaa caaactcgtc ggtacaaact gagaatctat attaactttt taaaaatctc
     6001 gacaaaatta tccgttgtca tttccaccga ttttccgtct tggatatcgg tggccagaca
     6061 atgtaccaat tggttgctga gaacggttac cgcgatctga gcgatggcgc tcttcaccgc
     6121 caccaattga atcaccaaat cctggcagga acgatcctcc tcaatcatct tttgaatccc
     6181 gcgcaactgt ccttcggccc ggcgcagccg ggctaatatt tcttccttgg tttccgtcgc
     6241 gaaacagagt tgttcattga ctcccgccat gcaaaagtcc ctccttgccg aaaacggctt
     6301 actttaccct gtaccgtatt atcttatcac aaaaaaactg tttctccaaa actgattgta
     6361 taaaagcaat tcaactatgg tccggcagag ggcgggtaat gtgggcagat tcatggtcga
     6421 tataagataa aaaaataaac ggcgggtttt cgcccgccgt tcattttttt aacgatagtt
     6481 acttttggac cggaagcaat ccctgcaaag gacttcacga tccggttttg gttggaacgg
     6541 cacttgggtt tgagcgccgc accgggtgca aaccgcgtca tacaatacgc gctcgctccg
     6601 gaacccgcta ccgcctctac ccgactggcc ccggccattg ttttttctag cctgacggca
     6661 actgggacac cggctaggct cgttggtaaa acccttttca gcgtaaaaat cctgctcact
     6721 cgctgtaaaa gtgaactcct gattacattc cttacaaact agagttttgt cactggacat
     6781 cttttcccct ccttatgaag ccttacggcc gatttgtctt cgcggatctc cggcttcgca
     6841 aacccgcgtt ttggcgccta actggcctca gatggagaaa agagtcgtct gaatctagcc
     6901 cgttaagtac tgacgctcac attataacat aaccttattc caacttcaat cggaaagcat
     6961 cccgttttgc ctccgaaaac ccgaataata ccaaattttt ctccggtttc cgtttcaaat
     7021 ctcgaatacc tttttttacc ttttaaaacc taatacacta tccccgcgat caaagtcaac
     7081 cctccgataa tcccccagac ccatagtttt aacttgagag gcataacaat cacctcattc
     7141 aaacactgag tttaaaaaga taggttatca catttatgca ggtcaaaata gatgcttaca
     7201 actctgggat tcctggacta tatccaaagt ttcgccgaac cgttgaaaat gaacaatctc
     7261 acgcacccgc aggaaattga gggtatcctt cagatccgga tcatcggtca aatcgatcag
     7321 ccactggtaa gttgccctgg ctttttgttc ggcagccata tcctcgtgga gatcggccac
     7381 aggatcccct ttcgcctgaa tgtatgttgc ggtccaggga ttgccggtgg cgtctacata
     7441 aaataacgca tgatcgtgat ccgcataatg cccgcccaaa tcaaccgcgc gcatctgctc
     7501 tgccgtggcg caccgggtta gttgatgaac cagtgtggcg atgatctcca aatgcgccaa
     7561 ttcctcggtg ccgatatcat tcaaaaccgc tttcgtttga ttggtgggca tcgagtatcg
     7621 ttggtttaga taacgcaacg ccgctgccaa ttctccgtcc ggcccgccat attgggtaat
     7681 caggtatttt gccatcttga gatcggcctt cccaacctta gccgggtact gtaacttttt
     7741 ttcataatgc cacatcgttt tccctccgat acttcttaaa tgttctggaa tagggattga
     7801 atcaaatcgt tcactcccga taatccagat cccaaggcca agggtcttgt gcccatcgcc
     7861 atacgggctg gttgacacta tcttccgctg tcagcggccc atagttcatt gtataaagct
     7921 gtctcacgcg attaacttca cacatcacat tggagtaatc ggtcagcgca cgttgatcac
     7981 ccggatgcgt attaagataa agactcagat ctaaagcggt aaattccaaa gcctgaagcg
     8041 ttttcaaaag ctccaattgc tcgcgttgca ccttaacccc tcctttctcg atcgcggctc
     8101 acatacggac gatagagctc cggaaagatc gtccccatca ttaatgcttt tttgggttcc
     8161 caacggttca caaatatctg taccggaaca tatgcctgag cgagctctag tgtaactcgg
     8221 ttcatcccgt ccatttcaaa tacccccttt aaccgtctat aacactatag tctattcgcc
     8281 cagggactaa tcgcctatat gacaatcgcc cgaaactcaa ggctttaggc ataaaaaaag
     8341 acccgtaaaa atacgggccg tttggtttcc gttcgaaaag gaaagtttta taaacatccg
     8401 atgattcgtc ccatcggata aaacattttt atcgggaatg acctggtttc gttgcggcct
     8461 gccggttttt gggcaccgct ccacgcttcc atttcattcg caccggctcg ggcatgagaa
     8521 tcttgtcaat ggagatcgcc cgatagtcgg cttcttccaa acaacggcaa cagttatcgc
     8581 agactttggt ttcatccaga tcacagatgt tgcagctgcc gcaaccgttg catatctttc
     8641 ccggctccaa gacgcattgg ctcattcttt accctccatt tcctatcaaa tcttccacac
     8701 gattacgaga gatcctttat tcatttttaa tctctctatt ttgccgctac acctcattcc
     8761 gcattcttcc cctctttccg gcctgcctga agaaagggat ggaagataac aaggtaaata
     8821 aaaacgactc accccctcac aatcttgaga tttcgttggt tatgagcttc cccctctttc
     8881 cggcctgccc ggagagaggg gaagcatcaa tgtggcttcg acctaccggc taactggaga
     8941 ggtgagtcgc aaattggata aagaagtcca ggttgaaatt aagcgggtca aaagttaagc
     9001 gaaattattg aatacattcc gataataaaa ccggtgaaaa gaaatagctc caatcttatg
     9061 aagaattgga gcttttcaat acagagtgat tactgaaaaa gtcttgcggt attaaatatt
     9121 tcttccccag acagggcagc gccctttcat ctccctaaac aaaagtcaga accaaattgt
     9181 ccaaaagagt tttacaaagc tttctgaagg aaaaacgtcc gggtacgaga agatgtatat
     9241 agtttagtca gtctaaatcc gcccgttgtt tcgtagaatg taccggaggc caatctgcga
     9301 acagacgccc ggaaagacag tatcgcctta ttttcggaaa ccggttggag cggccttccg
     9361 caaaataaag gacatggcaa ctgtattcaa ggaggaaatt catgctttac gctgcttcag
     9421 tcaaggtatg ctgcggcaag aaccaccgcc gcatcgatgt aatcgtggag gcggatgatc
     9481 cggaaaaagc caaagccaaa gtggtaaaac aagcccggaa aatttatgct ccgggcaaga
     9541 aagcaacgta cgaaatcatc ggaatggtca gtgaagccga ggccttcgaa aaattcttcg
     9601 ccaggatgaa ggaaactacc gaagccgagg gaccggatgc cgggtcagct ccagatgaac
     9661 cgtctcccaa ttctgaactc gatttgcccg gtccccggat ggaataagtc ggaaaaatta
     9721 tactctagac cccaatattt ggttgacctc ctgattccct ttattaagga actccgattc
     9781 cttgggatct aaaatctgga tgatggagag taactccccc acatgttgct tctcttcatc
     9841 ccggattgaa ctcaagaccc gctttacccg ttcatccgta gtcgccatta catgggcatc
     9901 ataaccgatg attgcctcca attccccggc cagatcggcc cggagcgctt gagccagttc
     9961 ttcccggcta atctggcgcg gaatattacc cacaaaggga ttacctaaat ccgccatctc
    10021 tcaaaacccc tttcaagttt ccccattgat tgactgccaa aatagcctgc ccggaaccat
    10081 ataatttatc cggcaagggg cattgagaga tagctttccg ggcagattgg ggcttggctt
    10141 cggataaatc ggcttttcaa agccgctcgc tttttcaaag ccactggctt catatcgggc
    10201 atataaaaag ccgggttcat aaaaaaccgg cacatggaaa gattaatgac cgggggtggt
    10261 atcaaatgga caaaaaaaat cagattgaag ccatcgtcga ttttgtcgga aaacatccgc
    10321 agactgtggc cagccggcgg atttgccgag aggtgctcgg agagggaatg gaacggtcca
    10381 atcaggaatt ctcttccgaa ttgacctcga aacttggaag cagggatgaa gagcagatca
    10441 gttcatacta ctatctgatc cgctaaccga cattcgttcg tatttgacat accgaaccgc
    10501 agtccggcct tggggacggc gaaatttact cccccttgtg ttgcatttcg tgaaagatct
    10561 tggtaaattc ggctgcgtgc tctttttcct tattcatgat cgccgtcaac aactgccgga
    10621 tgctttggtt attggtggcc tgaatctgcc tctggtaagc gttgatggca tggagttcat
    10681 cgcggatcgc cttccgtaag caatccagat cgcgtagatc gctctcaaaa cgccggtgtt
    10741 ccccggcagt gttctttttg gcctcgcgat aatccccgga ggtattcccg caaccggcac
    10801 acggccgccc catggctata gtcggttccg catacccggc caacatcacc aaaccctgtt
    10861 gtttaagttc ttccgcctga accggatcca aactgccaac catccggaac aaaccgataa
    10921 aatgcccggc ttcttcctta gcaatctccc ggaactgctc gcttatcctg cgatcgcgaa
    10981 tctcagcggc gcattcctga taggccacaa tcgcgcccaa ttcatccgcc agatcctccc
    11041 gtaaaaaaat caggtccggg tctccataaa attgagtcgt tcccagtgaa ggtcccagat
    11101 gcgggcagtc ataccggaat tccataccat tccctccagc gttccagata tctgccatat
    11161 gatatgtgct gcgggcttga aatgtgcgac agtcatctat tgggatccgg gtggcaaatt
    11221 attattctgc cggattcaag gttgtcagga ggaaaataaa gaaatagcaa gaaaatatta
    11281 ccgttatgag ttaattctta aaagtctatt atgggtgatc aactgatgtt tattttcaag
    11341 cgggtctatg gatacgcata tggaaagcaa cggcgaaccc gagcccttct acccctcatt
    11401 cttttcgctg cattactttt ggggcagatg gctcaagccg cctccggcaa actgatcgat
    11461 ctggatttta aaaattccga tctcaaagat atccttcggg ctttggcttc ccaagccggg
    11521 gtcacttttg tgattgataa tgatgtcacc ggtaccgtta cattgcatct tagcaaggtc
    11581 acctttggtc aggccctgga gatcctggcc aagaacaacc aactcaccta cactgtcgac
    11641 aataaagttt atcatatctc ccagattgat acctcggttc tcaaggtgga gtatgatgcc
    11701 gccgcagggt tactgactgt cgaagccgaa cgggttaaac tttttcgtct ctttgaaacg
    11761 ctctcccaaa agaccggagt aaacctggtc cccgctcccg aattgcaaga ccgaatcgat
    11821 attcaactgg aacgcgcccc tctcaccgat ggcattcagg ccattttgac caaagccaac
    11881 tgtatcgggg aaactatcgg taaagtaacg tatgtcagga aaaaagccac cgagttatac
    11941 ccgtttacaa ttaattatca gaataatcta attaccattg atgcccaaaa tattcccgta
    12001 agcgcggtaa cccgggcaat caccgaaaaa accggagtgg cggtagtcgc cgaccccggt
    12061 gtcaatagta acgtaagtat ctatattcaa aacctgccgc tcgccgaagc cctggcgatc
    12121 ctctccgaca gcaacgggtt ggaactgacc aaactcgata actcctggag aatctcccgt
    12181 aaagccggat cgttccgggt aagatttaaa gataacctat tatcggtaga tgccgacaat
    12241 gtcgatgtca ccgccctgac cacggagatc agccgtcaga cgggagtcaa tatcattctt
    12301 gcccgggaga tccggggaac agcgagcggt cattttcaaa acgttccctt gtcccaagct
    12361 ttgaacgcct tgcttgaacc tcaagggtgg gtggtggaac gccaatacgg cagttactat
    12421 ataaaaccca ataccggtca gaatcccaac cttcagataa gttatgatcc ggaaaagcaa
    12481 ctctttgaca tcacggttac tcaggcatcc ccgttgacca ccatcttagg tgagatagcc
    12541 cggaaggccg atctgaacat tgttatcatg ccccaagtga attggaatgt caataatctc
    12601 cgcctcaaag gaatgaacct ctctcaaacc ctgggttttc tacttcaggg tacggcttat
    12661 tcttgtacgg tgattgatca aacatatatc atcgcggata gcttatatcc ccgtcccgaa
    12721 aattccgact tccaaacggt caaggtttac cccgttcggt acctcaaggc ggatcagatc
    12781 ttaagtactc tgccggccat cttccccagg caaaatattg tgccactcca ggataaaaac
    12841 gccctggtcg taaccggaac cacggagctt cacagcaagt tcagcgaata cctggctcag
    12901 atggatgtgg cctccatcga ggatcagacg gaagtgatcc ggatcaaata cctcaaggcg
    12961 gaagatatac tgaaatatct gccctcatcg atttccaaaa cggatattat tgtaattaaa
    13021 gagatgaatg ctgtctcggt gaccggaccc caaaacctga ttaaccaggt taagcaatac
    13081 atcgataaaa tcgatcaagt caacccgatg atcgtctttg atatcctggt ggtctcgatc
    13141 tccgcttccg atacggttac ttgggatccc ggcaccgcat cgatacttac aggtacggga
    13201 ggtgtctccc tgactcccgg taccggaaag atcgtcaccg gagtgatgga tagtagttca
    13261 atcctctcaa gtatcaacct tttaatctct caaaacaagg ctaaagtact tcaaaacccg
    13321 acgatctaca cactaaacgg ttatccgacc aatttcaacg tatctaaaaa ggtcagttac
    13381 aatattgttg aaaacatcgg gaccggaacc agcaccacgc caaccagtcg ccaggttaat
    13441 ttcgactccg gcttgttcat cacgatgact ccgtgggtct cggccaatgg ccagatcacg
    13501 ctggagatca agcctacctt tagcacgttt gatccgggcg ggttaaccgg aaccgctgct
    13561 accacaggaa tcatcggcac aaccaacgag cgtaccgttg cggccacggt tcggatgcag
    13621 gaccaggaaa ctttggtctt gggcggattg aagtccacca accgtgaaaa gatcatttct
    13681 aaaattccga tcctgggtga tatcccgctc ttgggattcc tctttcgaac cactacttac
    13741 agggatgttc aggatgaatt cgtgatcttg gccactccga ggctgatcta tgatgaagcg
    13801 gacaaaaagg ccgctcaaga gaagtttgag gcggccttag gtcctgatac tcaaaatact
    13861 ttaagcaaca aataactcca ttcgcagttt acagattcac tctccccgtt ccggtctggt
    13921 tcttcaccac gatataattg gaaccgatgg tgataatctt ttctccggca aaaacttccc
    13981 ccacccggac taactgcgtg gccgtattgg aattgggatc aagccccact acagcggtct
    14041 cgcctgcaat cgttccccac aggattaaac tggaatgaaa ggagatgccg gtttcagccg
    14101 gtacgggatt ttggctaacg gctcccccga aaaaaagccg gtccccgggg agggaatcgg
    14161 ttcggccctg atgatcagtc tccggcagtc ggtaggagta tttccccacc tgtgtctgga
    14221 caggcatact cggctggtaa aaccgggcga cgatccagga tgttaacccc caaacaaata
    14281 tccccccgct gactgcgaag atgcccagcc aatacttggt ggcaagcgcg attcgtatct
    14341 gcttcattgc ttccttcaac cgactttcat ttcagtatcg ttcatgacta acgcagtaga
    14401 ctgatgatcc cgatctcata ctcgagttgc cgttcttccg gcagactatg aatccttaaa
    14461 tttttgatct tcaatgcaat cggtgtttcc ccgagccttt ccatgaaaag aaagagtgct
    14521 gcggagccgg tgcggccttc caaagtgacc tcaatcaacc tgtccggaag acgggcaata
    14581 ctttttttat ccaccgccag gccgctccgg gaggcaatag attccacctc ctgcaataaa
    14641 tcaagttgtg cccggtcaat ggatatttcc gggtaaaatc tggcttcgag tttggccagg
    14701 gctgcagtgg ccgcccgatt ccgggctatg attaccccgg atgaactcag tagccgttca
    14761 gcctctttct cattcagggc attaccgatc acaccgacat tttttgcacc gccgctgaaa
    14821 taattaaagc tcagtccggc aaggatcacc actcccacca ggagcagcaa ttttttttcg
    14881 cgatcagtca tgagtctcct tatctttttg aaccggtgtt ttctgggtaa cggtatcttt
    14941 aggggagccg gccaccggtc cctgaagatg aaaaacctct ccccggttgc ttgtcataat
    15001 cgtccctttc agcatcaacc ctgcaagctc cggaacttgg cgcagttgtt tcaataccag
    15061 acttgccgcc ggggcggtca aggccagctc cttcacgact ccgtcctcaa tggtgagatc
    15121 ggtgacaacc gttccttcgg gcagattatc ctggagagtt cgaagccacg ctaattcctg
    15181 cccttgatct tccagccagg cctcaaggtt ccgcatccgt cctagcgcaa ctccggtctg
    15241 attctcggtt ctctttagcc tttgcagata ctggctttgt tccccgagcc aacgcccaat
    15301 cttaacctct ttttcaaccc ctgcctgaaa cttcaggcct aaaccaatca agagcaaaca
    15361 ggcggcgact cccgccaatg cgatggagcg ccattttccc ttttgattgc gaatctgttg
    15421 ctgacgggca ctgtagatat aacggctttc atggtgataa aagcctaaaa cttcaaggcc
    15481 caatcccaaa agcggcgcta agcgggaagt aaatttacgg ttcaatgctc cgtgaatccg
    15541 ggaatcattc gcagcatgcc ctgagctaga actggctgcc cgcgaaacat cccggatttc
    15601 atcggggagt cgcaggcgaa ccccggcacc ggctgcggaa tcttgcagcg gctttgaaaa
    15661 ctcatcggtc tcacccaata accataaagg ctccggcaga gtcaaaccgc ttaacccctg
    15721 ataggcggca tgagacaact gaaattgctc ccaaaaatcg ttcaacaccg gctgggccgg
    15781 atcgctttca gaaacttccg gactgatcga agcagcttca aaagcggtcc ttaattttcg
    15841 ggctcccggc ataaaactgc ggcagtaaac caaccgttca ttttgaatca ctccaagctc
    15901 cgtctgttcc ggagtgatat ccagaaatgc aacactggcc gcagactcag taaagaagtc
    15961 gcgccggaag ttgagaaaat tctcattccc ccaatttcga agtcccgacc agagaatcgt
    16021 caaaccggcc cgctccagta attcaaagag ttccgtaagg gactgatccg gaataaccgc
    16081 cgcccgaaca tgatacattc ggtccctgag gcgatgatat aacggtttta tctgatgaag
    16141 gccgtggaga tcggacccgt tctccaactc cagccgaacc gcggcttcca gctgttcatc
    16201 cggaagcacc ggaaccacaa cagtatgata atgtacaaga tgggtcggga tgagacagat
    16261 gacacgatta ccccccggtc gctcggtttc ccaggtttgt cgcagccatt cgctgtccaa
    16321 tcccgtggaa ggcagcggcc ggacaaaatg cctgcatagc tcgatgccgc tctgtcgtga
    16381 cctaagttcc gctacggcca atacctcggg gccaggttca atcacgattg ccgtcctcat
    16441 gtttcacctc cggcttgaga ttaaagggta aagggtgaaa tagaacccaa accacctctt
    16501 gctcttctca tgcctaatgc ttcacacctt ttgcctttcg cctgttgcct cgcgccccac
    16561 ggggcgcgct actcatgcct catgcctcac gcctaaatca ccaaaaaaac cacccacgcc
    16621 gctccggcta agaatggacc gaagggcacc ggttggcgcg gcctaatctt ctgcgccagc
    16681 ttgaacggga ggaggatcgc gaagcctaaa aaactcccca aaaatagtac tccgatcacg
    16741 ctacgccagt cgcaaaaggc gccgatcatc gccagcattt tgatatctcc ccagcccata
    16801 cccgcgggct cgatcagccg gatgagccaa agtatcccaa aaccggccag tattccggag
    16861 agtcccaaaa accattgacc ttgccagaaa cgaactgcca atcctaacag caataaagga
    16921 atcgtcagcc catcggggac aatcatctgt tccaggtcga taaaggcgat ggccaccaaa
    16981 gtgtataata gaatcaagat cgcaatcccg ccgacgtcca ccgggtacct taatccccag
    17041 agcagggtta gtccggcagt gagcaattct accaaaggat agcggacttt gatcctgact
    17101 ccgcaataac ggcaacgccc ccgaagccag caataactca ggactggaac cagatccagc
    17161 caagataggc catggctgca attcgggcaa tgagacggcg gccagcaaag tgactcatgc
    17221 cgtggcaaac gatgaatgac tacatttaaa aaactgccgc agacgagtcc gaatattatc
    17281 tgaagccacc agagttgcgg aacacctaac cccattccct tgcctccaca tcggattttt
    17341 cttaagcccc gtccggacgt gcctaaccca aaagtctggc aaagcgcctt ctcgtcaagg
    17401 gccggctccg aaccgccaaa tcaggattcg tcgcagttta ttcggatggt ggtgattctc
    17461 cctcggccgg ggggttctct atttctgccg gtggaggctg ggggagttcc ggatcattgc
    17521 cggtagagga gccgctccgc tcataccaag ccagggacct gattgtccag cttgccaggt
    17581 cacttgcccg agctcgttcc tgaatcgtat ccagataata cctgacactc cccttggata
    17641 tccaaacccg ataacggatc agtgtagaga tacagctaaa atagtcggaa cttatgaaat
    17701 gctgattttg attgagaaaa tagttttcga tttcattttg gttcgcaaag ggttgctggc
    17761 taattcgggc tgccagagcg gcggccaatt ccggccctat tccggcctgg ttcaacagga
    17821 cggctatccc ttccctggtc atgaagttca gattggaacc accgctaaac cgaaagaacg
    17881 gccggagccg gtctcgggtg gggattagga tcccatccaa cttcaggaag tcgtcaatat
    17941 tggtgaaacg ttgcaactga ccggtgtttg gatctttcgt tgcacggtaa tgtctaaaac
    18001 tgcctgtcat cgtcacgacc tcatcgggtg taaaacctac cgaactcaaa agttccccga
    18061 aggtttccgg ccgaatggcg ttgggattca gcagtccgtg gacggtaact ctatcggcaa
    18121 gcagatcgta aagtgcggca ccgcccttaa cctgattcaa ctcttccacc gtagagaaaa
    18181 aaccgttccg ggtttggtaa ggtttcgcca aaccttggta aaaatcgcgt tccgccccgg
    18241 agggacgggg ttggtcatct ccatcgcgcc aatccaaaag cggatcgaag gatatctccg
    18301 tctcggattg accggcggta agcaggccca aatccttttg atcaagcgtg ttcaagttgg
    18361 gtttggaccc ctcatcctcg atcaggacgg aaatctgaaa gccattctgt tccgtaatcg
    18421 ttcgaccacc gtgataccag gagtcttgcg ggctgtcggc cggggaatcg tcagcggcaa
    18481 gaagctccag ctcgctgcga aggatcgcca aacccaatgc ccggcatctt tccatctgtt
    18541 gctgctggga gacaagagcc cattccacct cggagcggta gaataagaag gtggcgatta
    18601 gccccaatac accgcagatg caggtgatca taaacaatgt cgatccctct tggccatccc
    18661 tactcatggc tgaccgcccc ggggatcgga aaaaccagtc gccccagatc aaaatcccgg
    18721 gtcttcacgg tcaccccgat aagtgccggg atccggtacg attgttgtgg atcccagctt
    18781 aaaagccagt tttcggagac cggatcgaaa tattcaaaat gccattccga aactgttttc
    18841 aagaccggtt gttccacagc attttcgccc caaaaaccgg tggcccggtt aactgttccg
    18901 ctggctgaat caaaattgta ttgtaccttg accaccccgg aggatgagcg gctccaaaac
    18961 tccaatttgg actggtcacc ttggattgcc gcctccggca gatattgccc ggtaattaga
    19021 gcgccaagat cgtttcggag ccagcgatcg atcaataaca gctggtgttg gcgttcgtac
    19081 ccgcggccta tcctctccca aatcctcatc ccgccgaaga gcaattgggc cagaacccca
    19141 agaagaatcg cagtaaaact gatggccagg atcacttcgg gcagggtgaa tcccgattga
    19201 cctctgccgc ttatcggaac ggcgcttgat gaccctgagg ttaaccccgt ttcagtctgc
    19261 ggcccgataa ccactgaatg actcttcctg ggacaaacca ttctgattcc tccatttcag
    19321 cgtaagaatc agtcggctcc caccgtctgc cgcggtttcc tcccggcaag accaactaaa
    19381 gcgggagtaa ggaacagcaa aaactccgga agatcccttc tcgctacctt gttccagttc
    19441 ggcaagcttt cccctgccga taatcagcgc cgtaaccctt ccgcgtaact gttcctcggt
    19501 atagaccaac tgccggaagg accctaataa agccgagatc gccagggtca agaccattaa
    19561 agccacaatg acttcaaaga gggtaaaccc gtccggattc aacccggttc ctccggt
//
LOCUS       DASVMB010000074         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_231265,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000074 DASVMB010000000
VERSION     DASVMB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231265"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..881
                     /locus_tag="VF531_02455"
     CDS             120..881
                     /locus_tag="VF531_02455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX7712858.1"
                     /translation="MNIKRIDKLIGSGAQAGVYSVDRKAIKVFKEHCPKSEAFCEAAI
                     HSMVEETGLPVPKIYEVIQFENKMAIVMDLVEGASMERIVLNDLNNVSVYIDSIIDLQ
                     IKINSINAFGFPSMKDRLTRRISDSSSLNNLQKEKLQVILNSFDIGDRLCHGDFHLQN
                     LMMTDNGMIIIDWIDATSGSPEADLCRTYMLYKLYSPNGFADMYLDIYCRKTNQSRDD
                     ILKWLPIIAGARLSEKNESERDHLFQWVDMNSLLG"
     gene            918..1343
                     /locus_tag="VF531_02460"
     CDS             918..1343
                     /locus_tag="VF531_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7712859.1"
                     /translation="MEKLSKVFFDGREILFIGYSGKYESFCKSVFQTLTNHGIKVYPI
                     NNRSNANFDIKVYRDFSELPKIPSFAYVLTNQESTRKLIPELKKYDIQRILFQSKKNV
                     DQAALDECRKLGIETAVGCPMMLFGSGIHKLHAFFAGVR"
     gene            1340..3160
                     /locus_tag="VF531_02465"
     CDS             1340..3160
                     /locus_tag="VF531_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX7712860.1"
                     /translation="MRTFPVIRKTRLSNEHIMAGVFGVTLLYLLPSWIHSSAEILSYI
                     LVLITALFVDVAANYIRFKRPVCAVSAVVTAGILQVLTPGVPLWGRLLGITIALIFGK
                     HVWGGTGKNIVNPAIVGVLFLSLVFKLNLPLWAGSSWLWIALVLSLPFVLFRPFAAIG
                     LMAGMALSLLLHQQLSVATFISYGIIFWGSLVVTDPVTTTSVPSAGLIGGFLVGFLPL
                     CFTNSVAVMALTFLLADLAWYIYGVKSPIINGFGFTRPLKIKKLTPYNEGKIDFLDLT
                     GLGSSSPENIELPSPQEILNRIQTSGVFGFGGAAFPTDKKIRSVMDAGVSDKYFIING
                     VECDPGLLHDQWLIRRFPDEIGKGIEAICRYISFKKVILAVKDQGGLQSFKNTEIIKV
                     PDTYPAGAEKLLIGKVLQKELATDEVPAKKGILVLNIQTIYAIYEAVYLNRQAETRYL
                     TVANLKTKVGRVVKVNLGERVATILERLNLNSNMFFCGGGAMQSHLAEEGEVVGELTN
                     FIAMADFPRYKESPFCSHCGLCVEHCPAELSVNKIAELVSAGHIEATLKYHPERCMKC
                     GSCSYVCLAGRNLSAKVGEAADYGKSKIGTRQPKDAEPYR"
     gene            complement(3419..4069)
                     /locus_tag="VF531_02470"
     CDS             complement(3419..4069)
                     /locus_tag="VF531_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7712861.1"
                     /translation="MNQLSRREKEKMAREADIINAAEKIFCLKGFNEASLEEIAGEAQ
                     FTRRTLYQYFVNKEDLFFAVVLKGFRQMLAYTESLITKELSGFDKLYRMGLGYYRYYK
                     DFPEMLSLFNYIGYINKNPQSSPYYQKWRTFDNVMFTKLAGIIDEGKADRSIRTDLDS
                     QKAAYTLTFTLTGFFCQLSLTGPTFTAHFALDQEQFVLSSLNLLFDAFHARVNPLK"
     gene            4259..5050
                     /locus_tag="VF531_02475"
     CDS             4259..5050
                     /locus_tag="VF531_02475"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7712862.1"
                     /translation="MQTKIFYFSATGNSLAVARSIAAGIGEAELVSIPGVMAGEIDMA
                     VPKIGLVFPVYAYGMPRIVADFVRKLKLDKNNYVFAVATGGGTPANTLIQLRRLLRKR
                     GGDLDAGFAVKEANHTPLSKEDFLIKSMSRIAGKQPLTSRERLPEIIEVIKNNRKHRS
                     ETSSRVAGFIGDLLYKMAIATFKTADRDFLIDEKCGLCQTCIRICPRQNIKIEANKPT
                     WHHNCESCFACLEWCPNEAIQYKNVTQNQKRSHHPGIKVSEVILR"
     gene            5070..>5461
                     /locus_tag="VF531_02480"
     CDS             5070..>5461
                     /locus_tag="VF531_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX7712863.1"
                     /translation="MEHFQLSSKAFQDGGDIPERYSSTRKGQNISPPLHWVDSPAGTK
                     SFALIADDLDIPFGFIFTHWVVYNIPALKQELQEGVPHQRTFSDGTIQGKNSFRKNEY
                     LGPNPIVGGKHRYVFAIYALDKMLNPDP"
BASE COUNT         1551 a         1080 c         1285 g         1545 t
ORIGIN      
        1 ttccaaaagt tttttgaatt gaatctttcc aaaaataata accgttatat cgccccggga
       61 ttcaaaccgg aaatcttcgg gcggaacaaa caaagttagt attttttagt ggagggttca
      121 tgaacataaa aagaattgat aagttaattg gaagcggagc tcaagcggga gtctactctg
      181 tagatagaaa agcaattaaa gtatttaaag agcattgtcc aaaatccgaa gccttttgtg
      241 aagcggcaat ccattccatg gttgaggaga ccgggctacc ggtaccaaag atttacgaag
      301 tcattcaatt tgagaataaa atggcaatcg taatggatct ggttgaaggc gcgtccatgg
      361 agcggattgt attgaatgat ttgaacaatg tttccgtcta tatcgattca atcatcgatt
      421 tacaaataaa aattaatagt atcaatgcat ttggatttcc ctcgatgaaa gacaggttaa
      481 cacgacgcat atctgattcg tcatcgttaa ataatctcca aaaagagaaa ttgcaggtca
      541 tattaaacag ttttgatata ggcgatcgtt tgtgtcatgg cgattttcat cttcaaaatt
      601 tgatgatgac ggataatgga atgattatca tcgattggat cgatgcaaca tcagggagtc
      661 ctgaagctga tctgtgccgg acgtatatgc tttataagct atattcccct aatggctttg
      721 cagatatgta tttggatata tattgcagga agactaatca aagtcgtgac gacatactga
      781 agtggttgcc tataattgcc ggcgctaggt tgtccgagaa gaacgagtcg gaaagagacc
      841 atttattcca atgggtggat atgaacagtt tattggggta gaatgatcaa aaataactat
      901 taagggagaa atgaaatatg gagaaactta gtaaggtttt ttttgacggc agggaaattc
      961 ttttcatcgg ttattccggc aaatatgaaa gtttttgcaa aagtgtattt caaacattaa
     1021 caaatcacgg tatcaaggtg tatccaatca ataatcgctc taatgctaat ttcgacatca
     1081 aagtataccg ggatttcagc gaattgccga aaataccttc atttgcctat gtactcacca
     1141 atcaagagag cacccgtaaa ttgattccgg agctcaagaa atacgatatt cagaggatat
     1201 tgtttcagag taagaaaaac gtagaccagg ccgctctgga tgaatgccgg aaattaggga
     1261 tcgaaaccgc cgttggttgt ccgatgatgc tctttggttc gggaatccat aaactccatg
     1321 ctttttttgc aggtgtgcga tgaggacttt tcccgttata agaaagaccc gtctatccaa
     1381 cgaacatatc atggccgggg ttttcggagt cactttgttg tatctgcttc catcgtggat
     1441 tcattcttca gccgaaattc tcagttatat attggtattg ataactgccc tatttgtcga
     1501 tgtagcggct aattacattc gttttaagcg cccggtctgc gcagtctcgg ctgtggtgac
     1561 agccggtatt ttgcaggtat tgactcccgg ggttccgctc tggggcaggc ttttaggaat
     1621 aacaatcgcc ttgatcttcg gtaaacatgt ctggggtggg accgggaaaa acattgttaa
     1681 cccggcaatt gtgggtgtat tgtttttgag cctcgtcttt aaactgaacc tcccgttatg
     1741 ggctggttca tcgtggctct ggattgcgct ggtgctctcg ttacccttcg tgttatttcg
     1801 gccttttgcc gccattggat tgatggcggg tatggctcta tcgcttctgt tgcatcaaca
     1861 gttatctgtc gcaacgttta tttcgtatgg gattattttc tggggttctc tggtcgtgac
     1921 tgatcccgta accacgactt cagtaccttc ggccgggtta atcggcgggt ttttagtcgg
     1981 ttttttgccc ttatgcttca ccaactcggt cgctgtgatg gcgctgacat tcctgcttgc
     2041 cgatctggct tggtacattt atggggtgaa atcgccaatc atcaacggtt ttggttttac
     2101 gcgtccgtta aaaatcaaga aattgacgcc ttataacgaa gggaaaatcg attttctgga
     2161 tttgacaggg ctaggttcct ctagcccgga aaacattgaa ctaccttcac cccaagaaat
     2221 tttgaaccgg atacaaacta gcggagtatt cggctttgga ggagcagcct ttcccaccga
     2281 taagaaaata agatcagtca tggatgccgg tgtttccgat aaatacttca tcatcaacgg
     2341 ggtagaatgc gaccccgggc tcctccatga tcaatggttg attcgaagat tccctgatga
     2401 aatcgggaaa ggtatcgagg cgatttgccg gtacatttct tttaaaaagg ttattttggc
     2461 agttaaagat cagggtggtc tgcaatcctt caaaaatacc gaaattatca aagtacccga
     2521 tacctaccct gccggggctg aaaagctttt aatcggtaaa gttttacaaa aggaactggc
     2581 caccgatgaa gtcccggcca aaaaagggat attggtactc aatatccaga caatatatgc
     2641 aatctacgag gcagtctatt tgaaccgtca agctgagact cgatacctga ctgtcgctaa
     2701 ccttaaaacc aaagtaggcc gggtggtaaa agttaacctg ggagaaagag tagctacgat
     2761 tcttgaacga cttaatttaa acagtaatat gtttttttgc ggcggcgggg cgatgcaatc
     2821 ccatttggcg gaagagggag aagttgtggg agaactgact aacttcatcg ctatggctga
     2881 ttttccgcgt tacaaagagt cgccgttctg ttcacattgc ggtttatgcg tggagcattg
     2941 cccggctgaa ttaagcgtga ataaaatcgc ggaattagtt tctgccgggc atatcgaggc
     3001 caccttgaaa tatcacccgg aaagatgcat gaaatgcggc agttgcagtt atgtttgtct
     3061 agcgggcaga aacctttctg ccaaagtagg agaagccgca gattatggaa agtcgaagat
     3121 tggaactcgt caaccgaaag atgctgagcc ttatcgctaa gaggtaattg atttcagccg
     3181 gggagtcact ggcctgatat agggtggaaa atgcatggag cggcaacgcg cacgtgcgct
     3241 caagaactaa aaaagacgat ggaacggttt gaaccatgcc cgccggtgac accgattccc
     3301 gggcgcggtt tcatgccacg aatttcacct aaggaggttt tgattacaac attctgaagt
     3361 gattgccgac aattgccggc gccaggttat ccgaaagaat aagccggaaa aggaccaatt
     3421 atttcaatgg gttgacccgg gcgtgaaagg cgtcaaacag tagattaagt gaagataaca
     3481 caaattgctc ttggtccagc gcaaaatgag ccgtgaatgt cggaccggtc aatgaaagtt
     3541 ggcaaaagaa tccggtcaag gtaaaggtta aggtatatgc agccttttgg gagtccaaat
     3601 ccgtccgtat actcctgtcg gcttttcctt catcaataat tcccgcaagt tttgtaaaca
     3661 ttacattgtc aaatgtccgc catttttgat agtaagggct gctttgcggg ttcttgttga
     3721 tataaccgat atagttaaac aaactaagca tttcgggaaa atctttataa taccggtagt
     3781 accctaaacc catacgataa agcttgtcaa atccgctaag ttccttggta atcagggatt
     3841 cggtataggc cagcatctgt ctaaatccct tcaaaaccac ggcgaaaaat aaatcttctt
     3901 tattcacaaa atattggtaa agggttcttc gagtaaactg agcttccccg gctatctctt
     3961 ccagggaagc ctcattaaaa cccttcaggc aaaatatttt ttctgcggca ttgatgatat
     4021 ccgcctctct ggccatcttt tccttttctc tccgggatag ttgattcata gatatttatc
     4081 tcctttgact tgacagaaag tatacacatt gtgtattata tatccataat gtaatcgcga
     4141 tatactaaaa gtatattatt tcaggcttgg gttgtcaagc agaatcggga atcgtcgtaa
     4201 ggagtggtgg ccaatgaaag aatcctatga aatagacaaa atacctggag gtctaaattt
     4261 gcaaacgaaa attttctatt tttccgccac aggtaattct ttagcggttg cccgaagtat
     4321 cgccgccgga attggtgaag ccgaattggt ttcaattcct ggcgtgatgg cgggggagat
     4381 tgatatggct gtgccaaaga ttggtttggt ttttccggta tacgcctatg gtatgcccag
     4441 gattgtcgct gattttgtga gaaaattaaa attggataag aacaattatg ttttcgcagt
     4501 ggctaccgga ggtggaacac cggcaaacac attgattcag ctcagaagat tgctgcgtaa
     4561 aaggggtggg gacctggatg ccggatttgc tgtgaaagaa gcgaatcata ccccgcttag
     4621 caaggaagac tttttaatca agtctatgag tcgtattgcc gggaaacaac cgcttacttc
     4681 cagggaaaga ctccccgaga ttattgaagt cattaaaaac aatcgcaaac atcgatccga
     4741 aaccagttcc cgggttgccg gctttatagg ggatttactc tataagatgg cgattgccac
     4801 ttttaaaaca gccgaccggg atttcctgat tgatgaaaaa tgcggtctct gtcaaacctg
     4861 tatcagaata tgcccgcgtc aaaatatcaa aattgaagcg aataaaccaa cttggcatca
     4921 taattgtgaa tcctgttttg cttgtctgga atggtgccca aatgaagcca ttcaatataa
     4981 aaacgtcact caaaatcaaa agcggagcca tcatccggga ataaaggtta gtgaggttat
     5041 tttgaggtga agggagagga agtaatcaaa tggaacattt tcagttatcc agcaaagctt
     5101 ttcaagatgg aggagatatt cctgagcgtt acagcagcac ccgcaagggt caaaatattt
     5161 ccccgccgtt acattgggtg gattcgcctg cgggaaccaa gagttttgct ttgattgcgg
     5221 atgaccttga tatacctttt ggctttatct ttacccattg ggtggtctat aatataccgg
     5281 ctctgaagca agaattacag gaaggcgttc ctcatcaaag aaccttttca gatggtacca
     5341 ttcagggtaa aaatagtttc aggaaaaatg agtatttagg ccccaatccg attgttgggg
     5401 gcaaacaccg gtatgttttt gccatctatg cactggataa gatgttaaat cccgatccga
     5461 a
//
LOCUS       DASVMB010000075         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_376464,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000075 DASVMB010000000
VERSION     DASVMB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376464"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1172
                     /locus_tag="VF531_02485"
     CDS             258..1172
                     /locus_tag="VF531_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7712864.1"
                     /translation="METIRVTELKKFYGRVRAVDGISFTVHPGEVFGMLGPNGAGKTT
                     TVEMIVGLTPRDSGDILVMGFDPAREPLDVKSRIGVQLQTAAMFPRLTVVETIRLYAD
                     FYPQPLPVGEAIDLVGLGEKANTQTIKLSGGQLQRLSVALALIGNGDAIFLDEPTTGL
                     DPQARRALWEVIRQMKQRRKTVFLTTHYMDEAEKLCDRVAVVDRGKIIALDSPADLIN
                     HHFPEKAIEFNLERSAAGESLNRLPGVKRVKYEEENAILYTTEVPVTIAALLHYSETA
                     GIPMNGLTIRTATLEDVFLELTGRRLRE"
     gene            1169..1465
                     /locus_tag="VF531_02490"
     CDS             1169..1465
                     /locus_tag="VF531_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712865.1"
                     /translation="MKGFWQLVTANVKDIVRDRMSIFWFLAFPLLFILLFGAIFGKDQ
                     ATTRYGIGLVVEDQTLWARPFKTPSGRCPFSSCIREPGSRNYRRWGGANGFWLW"
     gene            1348..2253
                     /locus_tag="VF531_02495"
     CDS             1348..2253
                     /locus_tag="VF531_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712866.1"
                     /translation="MGKTIQNAFRAVPIFELHQGTRVKELQALGRGKRFLVVVVPRHS
                     AGSGRTIAPLTIPVYFDPSKAATTQALIAAVREILAELERKMTGAPRLLSAQPIKATV
                     TQLKNIDYLLPGILAMALMQLGLFGALNFVSLRERKIIRRLGATPLPRSMMLASEIIV
                     RLGMAIIQTIVIVALGFLAFRVTITGNLLAVLGAVILGASVFITLGYFLVSFTKTEES
                     ASGLIQVVQFPMMFLSGIFFPLETMPGFLRPMVNAIPLTYLGDLLRKVMVGLPSAYGL
                     GTDLAVLGGWLIVVMVLAVRFFKWE"
     gene            complement(2369..>3441)
                     /locus_tag="VF531_02500"
     CDS             complement(2369..>3441)
                     /locus_tag="VF531_02500"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX7712867.1"
                     /translation="TRVKLLAFENIPYPPEIRPRIAELFDPETGRVDRICRMNFVIGE
                     LFASAALQIVAKAGLEAEEVDLIGSHGQTIYHLPEFHKGDGIGTGSTLQIGEPAVIAE
                     RTGIITVADFRVGDVAAGGQGAPLVAYADHVLFGLTPKVRIIQNIGGIGNLTLVGGQI
                     IQSQVMAFDTGPGNMILDELAGIITDGREHFDRDGKLAGAGKVNQSLLAKLLNHSYFM
                     KTPPKTTGREEFGTGYVRDLYQKARGLGICDEDLMATATAFTVESIAYHYEKYIFPHH
                     RVDEVIVGGGGVYNPTLMEMLRRRLAPVPLLCHEDFGIVSDAKEAIAFAILANETVCG
                     NPSNVPSATGATKEAVLGKIVL"
BASE COUNT          801 a          908 c          926 g          806 t
ORIGIN      
        1 tgctaaaata ctaaatttct acaaattgac atccatctcc tgcttaaaat ctccaaagac
       61 cgcaatcatc aaaaccatga catattattc gcctcgatta accgtccccg ccactgttgc
      121 caaataatta agcccgaccc gggagatgaa tagagtattc caggagattt tacccgaaca
      181 tcgaattata aaaccggaaa atcagtcgaa aacaacgatt tatcggttaa ccggaacaga
      241 aaggcggaaa caaacctttg gaaaccatcc gggttacgga actaaaaaaa ttttatggcc
      301 gggtcagggc ggtagacgga atatccttca cagtccaccc cggagaggtc tttgggatgc
      361 tcggcccaaa cggggccgga aagaccacca ccgtggaaat gatcgtcggg ctaaccccca
      421 gggattcggg ggatatccta gtaatgggtt ttgatccggc ccgcgagccg cttgacgtca
      481 agtcgcgaat cggggtgcaa ctccagacag cggcaatgtt tccccggctc acagtggtcg
      541 agaccattcg actttacgcc gacttttatc cccaaccgtt accggtcggc gaggcgattg
      601 atctggtcgg actgggagaa aaggcgaata cccaaacaat caaattaagc gggggtcaat
      661 tacaaagatt aagcgtcgct ctggcgctga tcggcaacgg ggacgcaatc ttcttggatg
      721 aacccaccac cggactggac ccccaggccc gccgggcgct gtgggaggta atcaggcaaa
      781 tgaaacaacg gagaaaaacc gtatttctga caactcatta catggacgag gcggaaaaac
      841 tctgcgaccg ggtggccgtt gtggaccggg ggaagattat tgctctggac agcccggcag
      901 atttgattaa ccatcatttt ccggagaaag ccatcgagtt taacctggaa cggtcggctg
      961 ccggcgaatc cttaaaccgg cttcccgggg tgaaacgggt caaatacgag gaagaaaatg
     1021 cgattttata taccacggag gtccccgtca ccatcgctgc cttgcttcat tattcggaga
     1081 cagcagggat ccctatgaac ggtttgacca tacgtaccgc cactctggaa gatgtctttc
     1141 tggaactgac cggaaggagg ttgcgcgaat gaaaggcttt tggcagttgg tcacggccaa
     1201 tgtgaaagat atcgttcgcg accggatgtc catcttctgg tttttggcct ttccgctcct
     1261 attcatcttg cttttcggtg ccattttcgg caaggatcaa gccactaccc gttatggaat
     1321 cggtctggtc gtcgaggatc agaccctttg ggcaagacca ttcaaaacgc cttccgggcg
     1381 gtgcccattt tcgagctgca tcagggaacc cgggtcaagg aactacaggc gctggggcgg
     1441 ggcaaacggt ttttggttgt ggtagttccg cggcactcgg ctgggagtgg gcggaccatt
     1501 gcaccactta cgattccggt ttatttcgat ccgagtaaag ccgcaactac tcaagctttg
     1561 atcgctgcag tccgggagat cctggcggag ctggaacgga aaatgaccgg agcccccaga
     1621 ttactgtcgg cccaaccgat caaagccaca gtgacccagt tgaagaatat cgattacctc
     1681 ttgccgggta tcttggccat ggctttgatg caattggggc tctttggggc tttgaacttc
     1741 gttagcctac gggagcgcaa gatcatccgc aggctcggcg cgactccgtt accccggagt
     1801 atgatgctgg caagcgagat tatcgtccgg ttgggcatgg ccatcattca gacgattgtt
     1861 atcgttgccc tgggattctt ggcctttaga gtcacgatca ccggcaatct tctggcggta
     1921 ctgggggctg tgattctagg cgcatcagtc tttatcaccc tcggctattt cctggtcagc
     1981 ttcaccaaga ctgaagagag cgcctcgggt ctaatccagg tggttcaatt cccgatgatg
     2041 ttcctttcgg ggatcttctt ccccttagag acgatgccgg gttttttaag gccgatggtt
     2101 aacgccatcc ccctgacata cctcggggat ttgttgagaa aagtgatggt gggcttaccc
     2161 tccgcttacg ggttgggcac cgatctggcg gtgctgggtg gctggctgat tgtggtgatg
     2221 gttctggcag tccggttttt taaatgggag tgaaacgtct tgcccaacaa gtacgaggca
     2281 acagggacta ggcaacgggc aataggtaag gactttggac taaaataatc gacataaata
     2341 ctgttgcctg ttgcctcatg cctgttgcct atagcacaat cttccccagc accgcttcct
     2401 tggtcgctcc ggtagcagac gggacattgg aaggattacc gcagaccgtt tcattggcga
     2461 gtatggcgaa ggcaattgct tccttggcgt cgctaacgat tccgaaatct tcgtggcaca
     2521 ggagcggaac cggtgccaga cgtcgccgca gcatttccat taaggtggga ttgtagacac
     2581 cgccgccccc tacgatcact tcatccaccc tgtgatgagg gaatatatat ttttcataat
     2641 gataagcgat ggattcaacc gtaaaggctg tggcggtcgc catcaaatcc tcgtcacata
     2701 ttccaagtcc ccgggccttt tggtaaagat cccggacgta tcccgtgccg aactcttcgc
     2761 ggccggtggt tttgggtgga gtcttcataa aataggaatg gttgagtagc tttgcaagca
     2821 gggactgatt cacttttcca gccccggcaa gcttcccgtc cctgtcaaaa tgctccctgc
     2881 catcggtaat gattccagcc agttcatcaa ggatcatatt tccggggccg gtgtcaaagg
     2941 ccattacctg gctttgaatg atctggccac ccaccaaagt gagattgcca ataccgccga
     3001 tattttgaat gattcttact ttcggggtga gaccgaacaa tacatggtcg gcataagcca
     3061 ccagcggcgc gccctgacct ccggcggcaa catccccgac ccggaagtcg gccacggtta
     3121 tgatcccggt ccgttcggca atcaccgccg gttctccaat ctgcagggtc gatccggtcc
     3181 cgatcccgtc ccctttgtga aattccggca gatgatagat ggtttggcca tgagaaccaa
     3241 tgaggtcaac ctcctccgct tccagtccgg ccttggccac gatctgcagg gcggcggagg
     3301 caaataactc cccgatcacg aaattcatgc ggcagatccg gtcgactctt ccggtttcgg
     3361 ggtcaaacaa ttcggcgatc cggggccgaa tctccggcgg atagggaata ttctcgaagg
     3421 ccaagagctt gacccgggta t
//
LOCUS       DASVMB010000076         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_84273,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000076 DASVMB010000000
VERSION     DASVMB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84273"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VF531_02505"
     CDS             complement(<1..240)
                     /locus_tag="VF531_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7712868.1"
                     /translation="MMKLGFLSGCLNNVPLEQLVKFASQTGFTALELSAWPIVNTRDY
                     SGSTIDVAHLTPVKADELKTLFREHNLAISSLAYYE"
     gene            378..1298
                     /locus_tag="VF531_02510"
     CDS             378..1298
                     /locus_tag="VF531_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7712869.1"
                     /translation="MPKASPGHDYTLHETMNMPNKDFPIRVFPCECQPAVQMFHAHWH
                     EQLELLCFIEGSAVVECNSNPVPVKAGELLVINSNELHAGYTLTEGALYYCIIIDISL
                     LESSFTDSCEIKYIAPITRNLILFNNRIGADREIESCIQQIVTEFKKQEIAYELAIKS
                     AIYHILTVLIRQKISRVLSTKEYERRSRSLERLRDVFRFISHNYTKKLDIETLAGMAH
                     MSPYYFCHLFKEVTGKTLSQFLNHKRINRAEFLLKTTTMTVTEIARESGFEEINYFSR
                     IYKQYKKVSPSAMRKRHGALEYQGPIRHEE"
     gene            complement(1364..1627)
                     /locus_tag="VF531_02515"
     CDS             complement(1364..1627)
                     /locus_tag="VF531_02515"
                     /inference="COORDINATES: protein motif:HMM:NF018584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HEX7712870.1"
                     /translation="MVENEKIGRLVTAHFNSREEFGENYIIGRRFWAENSKQEEQLAA
                     EAQTARNQLFSPTGAWKRIPWEWGKDTAQSDRASDIPGKIKKG"
     gene            1916..>3929
                     /gene="uvrA"
                     /locus_tag="VF531_02520"
     CDS             1916..>3929
                     /gene="uvrA"
                     /locus_tag="VF531_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7712871.1"
                     /translation="MSSNSIKIKGAREHNLKNIDLEIPRDQLVVLTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDYIEGLSPAISIDQKTTSHNPRSTVGT
                     VTEIYDYLRLLYARIGHPFCPNCHQPITQQTVEQIVDQVMALAEGTKFMVMAPVIRHR
                     KGEYDKLFEDLKREGFVRARVDGTIHDLSETIDLEKYKQHTIEVVVDRLVRKDDIGQR
                     LADSVELALKQAKGLVTIATMDGKETIYSEKFACPDCGFSFEELTPRMFSFNSPYGAC
                     PDCAGLGFKMEIDPELVIDKTRSLNDGAIKAWSSDQDSWSLQYLLSVAKKYKINPDSP
                     LKEASPEQLQILLYGTGGEKIEISYHGPDREFRHAAAFEGVAHNLERRYRETQSEYMR
                     REIEKFMTIKPCATCQGARLKRESLSVLVGELNLSQLTALSVLEAKAFLQKLDLTERE
                     TAIARLVLKEINERLGFLVNVGLDYLTLDRAAGTLSGGEAQRIRLATQIGSFLVGVLY
                     ILDEPSIGLHQRDNRRLIDTLKGLRDLGNTVVVVEHDEEMIREADYIVDIGPGAGVHG
                     GEVVAAGTLKEVMASPASLTGQFLSGKEQIAIPARRRKPNGKYLEIVGAREHNLKGID
                     VKIPLGVFVCITGVSGSGKSTLINDILYPVAATKLNGATTLQAGAYD"
BASE COUNT          983 a          957 c         1020 g          969 t
ORIGIN      
        1 ctcataatac gccaaagagg agatggccag attgtgttct ctgaatagcg ttttcaactc
       61 atccgccttg acgggggtca gatgggcaac atcaatcgtg ctccccgagt aatcccgggt
      121 gttcacaatc ggccaggccg atagttccag cgccgtaaac ccggtttggg aggcgaactt
      181 cactaattgt tccaggggca cattgttcag acagccgctt aaaaagccaa gcttcatcat
      241 tcatcctcct ttttatatca acctattttg ttattgaacc agccggttta taatcaatat
      301 aaaagtattt tccagaaaat tcaatgcatt gacttgttat aaattagcac tagattgcgg
      361 taaaggagag gatcttaatg cccaaggcga gccccggcca tgattatacg ctgcatgaga
      421 ccatgaatat gcccaacaaa gactttccga ttcgggtgtt tccttgcgaa tgtcaaccgg
      481 cggtacagat gtttcatgcc cattggcacg aacagcttga attgctctgt tttattgagg
      541 gttcagcggt ggttgaatgc aactccaacc ctgttcccgt taaggccgga gaattactgg
      601 tgatcaacag caacgaattg catgccggat acacccttac cgagggcgcg ttatactatt
      661 gcattatcat cgacatttcc ctgctggaaa gtagttttac agacagttgc gagatcaagt
      721 acattgcccc gattaccagg aatttgattc ttttcaataa tcggatcgga gccgaccggg
      781 agatagaaag ttgtatccag cagatcgtta ccgaattcaa gaaacaagag atcgcttacg
      841 aactcgccat caagtccgct atctaccata tcttgactgt gttaatacgg cagaagatct
      901 ccagggtgtt gagtacaaag gaatatgagc gccgctctcg gagtctggaa cgcctcaggg
      961 atgtcttccg gtttatatct cacaattaca cgaaaaaact cgatatcgaa acgctggccg
     1021 gcatggctca tatgagtccg tactatttct gccacctttt taaagaggtg accggaaaga
     1081 ccttgagcca attcttaaac cataaacgga tcaaccgggc ggagttcctt ttaaaaacga
     1141 ccaccatgac agttaccgag attgcccggg aatcgggatt cgaggagatc aactacttca
     1201 gtcggattta taaacaatat aaaaaagtat cgccctcggc catgaggaaa aggcacggtg
     1261 ctttagaata tcaaggcccg attcgtcacg aagaataaga tcacagcaat cggtgattca
     1321 atatcaagcg taaatggaaa ggggtgaatt gcagttttga agttcacccc tttttgattt
     1381 tccccggaat atccgaggct cgatcggact gagccgtgtc tttcccccat tcccagggga
     1441 ttcgtttcca agctccggtg ggtgagaaca attggttgcg cgcggtttga gcttccgcag
     1501 cgagctgctc ctcctgtttg gagttttcag cccagaacct gcggccgatg atatagtttt
     1561 ctccgaactc ctcccgggaa ttgaaatggg ccgttaccaa ccgcccgatc ttctcgtttt
     1621 ccaccatgag tttcctaccg gttgagagaa acttgttcca tgaaaaaatg agatttcagt
     1681 ctttttaacc actgtgcaaa ttcagccgga tgaatttttt tattccgatg gctcttccgg
     1741 gaaaaatgtt atatactagt gcctgtatac tgttcatatt gatgaaacca taccagagct
     1801 ttggatttag gacgatcttt aaccttgatc cggttagttc ggcgtatcag tttaacgttg
     1861 acaacaaaca tacgttcgtt tacaataaga ttagtcattt tttacggagg tcgtaatgtc
     1921 cagtaacagt attaagatca agggcgcccg tgagcataac ttgaaaaaca tcgacctgga
     1981 gatccctaga gaccagctgg tggtcttgac ggggctttcg ggatcgggta aatcttcttt
     2041 ggccttcgat accatttatg ccgaagggca gcgtcggtat gtggagtcgc tctcggccta
     2101 tgcccggcag tttctgggtc agatggacaa accggatgta gattatatcg aggggctctc
     2161 tccggccatc tccatcgatc aaaagactac cagccataac ccacgttcca cagtggggac
     2221 agtgacggag atctatgact acctgcggtt gctttacgcg cggatcggcc accccttttg
     2281 ccccaactgt catcagccca ttacccagca aactgtggaa cagattgtgg atcaagtgat
     2341 ggcgctggcg gagggaacca aatttatggt aatggccccg gtaatccgtc accgtaaggg
     2401 tgagtatgac aaattattcg aggatctgaa acgggaaggc ttcgtccggg cccgggtgga
     2461 cggaaccatt cacgatctct ccgaaacgat cgatcttgaa aagtataaac agcataccat
     2521 cgaagtggtg gttgatcgcc tggtccgcaa ggatgatatc ggacagcggc tggcggattc
     2581 tgtggaatta gcgttgaaac aggccaaggg actggtgacc atcgccacca tggatgggaa
     2641 ggagacaatt tacagcgaga agtttgcctg ccccgattgc gggttcagct tcgaagagtt
     2701 gacgccgcgg atgttctcct tcaacagccc gtatggagcc tgtcccgact gtgccgggct
     2761 cgggttcaag atggagatcg atccggagtt ggtcatcgat aagacccgat cgctgaatga
     2821 cggtgccatt aaggcctgga gctcggatca ggattcctgg tcccttcagt atctgctttc
     2881 ggtagccaaa aagtataaga tcaatcccga tagtccctta aaagaggctt ctccggaaca
     2941 actacaaatc ctgctctacg ggaccggggg cgagaagatc gaaatatcct atcacgggcc
     3001 ggatcgggag tttcgccatg ccgcagcttt cgagggcgta gcccataact tggagcgccg
     3061 ttacagagaa acccagtcgg agtatatgcg gcgcgagatt gaaaagttca tgaccattaa
     3121 accctgcgcc acctgccagg gtgcccgcct gaaacgggag agtctctcag tcctggtcgg
     3181 cgagttaaac ttaagtcagt tgaccgccct ttcggtcctg gaggccaagg cattcctgca
     3241 aaagctggat cttaccgagc gcgagacggc catcgcccgc ttggtattaa aagagatcaa
     3301 tgaacggttg ggttttttgg tcaatgtagg tttggactat ctgaccttgg accgggctgc
     3361 cggaaccctt tccggaggtg aggcgcaacg gatccggctg gcaacccaga tcggttcttt
     3421 cctggtggga gtcctctata tcctggatga gcccagtatc gggttacatc agcgtgataa
     3481 ccggcgactc atcgataccc ttaaggggtt gcgcgatctg gggaacaccg tggtcgtggt
     3541 agaacacgac gaagagatga tccgcgaggc cgattatatc gtggacatcg gccctggagc
     3601 tggcgttcac ggcggtgaag tggttgccgc cgggacatta aaggaagtca tggcatcgcc
     3661 cgcctcgctc acggggcagt tcctctccgg taaggaacag attgccattc cggcgcggcg
     3721 ccgcaaaccg aacggcaagt atctggagat cgtcggcgcc cgggagcata atttaaaggg
     3781 gattgatgtc aaaattcctt taggggtttt tgtatgcatc accggcgtct cgggctcggg
     3841 aaagagtacg ctgattaatg atattctcta tccggtggcc gccacaaagt tgaacggggc
     3901 taccacattg caggccggcg cttacgacc
//
LOCUS       DASVMB010000077         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_9339,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000077 DASVMB010000000
VERSION     DASVMB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9339"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1390)
                     /locus_tag="VF531_02525"
     CDS             complement(32..1390)
                     /locus_tag="VF531_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7712872.1"
                     /translation="MFRKLRLQFILTNLGIISALFVTLTVGAYAILEIKMINHAEVFS
                     KRLAMGINSGVFPGFPADDARPPRFEGMHDNPSHGIRKPPVLPPGPPRPTQTPPSPGF
                     FFVKTDFSGNMLFQSFREPWDALHLTIAIQSILKADKASGIVDLFNSKYHYYRAPLQK
                     ESGMLIVFQDLKQDKNIQHSLVVSLSIVGVVFFILAMLGSLFMAGRAIAPIQKAWRQQ
                     QDFLADASHELRTPLAIIQTNLEVVLSDPEETVASQADWLNNIQEELRQMTFLVSSLL
                     FLARADSKPNVLDSRDFALNQTVTRVSEAFKPAAAAKDIRIETTAMDKVICHGNESNI
                     RQVMEILLDKAVRHTPSGGRVKLDLGKTEKKIHLTVSDTGEGIPPEHLDKIFDRFYQV
                     DSSRSNGKAGLGLSIAKCIIENHGGTIRVASKPGQGTVFTILLPLPNARIRGNPSIKP
                     NP"
     gene            complement(1380..2054)
                     /locus_tag="VF531_02530"
     CDS             complement(1380..2054)
                     /locus_tag="VF531_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7712873.1"
                     /translation="MRLLLIEDEIKLAKALAHLLEKSGYIVDTATDGEKGLELAQNGN
                     YDLIILDRMLPGKDGIAIIKELRAEGLDMPVIFMTAKDAPKDRVEGLDAGADDYLVKP
                     FATEELLARLRALARRKNRELVGNTINAGGLILDPLKCMVTRGTEVIQLSVKEALLLE
                     LLMRNHGQVVTKELIFERVWGYGSETDFNNIDLYIHYLRKKLNPSCIVTVRGVGYFLK
                     GESDVS"
     gene            2274..2597
                     /locus_tag="VF531_02535"
     CDS             2274..2597
                     /locus_tag="VF531_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712874.1"
                     /translation="MNIASTTSGLSGSILTTGYNDNTLTQLEKEKSDLEAQLQKEQAS
                     QTDAQTKQQKIEQLQNQINQIEIRINQQKAAQQNKVADSFGDDSSAGSDSTLRTSSTQ
                     IDIKI"
     gene            complement(2681..>3918)
                     /locus_tag="VF531_02540"
     CDS             complement(2681..>3918)
                     /locus_tag="VF531_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7712875.1"
                     /translation="AVMQYLIDHGANVNTLGNGHSPLAWACHNSHHGYLEAVKLLLEK
                     EADINQSFRYEGKYADNPDNQWLRGWTPLMFAIHDWGATTDIIQYLLDHGANPKAVSE
                     NGETPLYVDFIVGNRLGNQMEIAKLLLAKGVDINGWVRDFGSVLHYAAMMGSSQAIKD
                     LLALGANINLPCNGGQTPLLTAIKSNEHSLETVRLLVENGADVNRGDDSGWTPLHEAC
                     SKGDLELVKYLISKGADKSLQNKDGATPFAVISQEHQDLIDYLKDWATIDWNQIKPLA
                     VDKVGDALDGATDLEAGYAVMDEGYLYLAAKTNSVDPMVDISIQCNGDQGNQFLVLFL
                     SNLNRADIFRIDQGKAVPIGSASLSYNPLETVIPLKMLGDPKSVTVNFAVKRIKGNDD
                     YRTYDEIPGWYEVKGNKQR"
BASE COUNT         1063 a          997 c          852 g         1006 t
ORIGIN      
        1 ttttttcata aaatgaaagc gatgaaagcg ttcacggatt aggtttaatc gatggattcc
       61 cgcggatacg cgcattgggt aaaggtaata gaatcgtaaa gaccgtaccc tgaccgggtt
      121 tactggcaac ccggatggtt cctccatgat tttcgatgat acatttggcg atagacaatc
      181 ccagacccgc tttaccattg gaacgggagg aatccacctg ataaaaacga tcaaagatct
      241 tatccaagtg ttccggaggg attccttctc cggtatcgga aacagttaag tggattttct
      301 tctccgtttt acctagatcg agcttaaccc ggcctccgga tggagtatgc cggaccgctt
      361 tatcaagcag aatttccatg acctgccgga tatttgattc gttcccgtga caaatcacct
      421 tatccatagc ggttgtctcg atccgtatat ctttggcggc agcagccggt ttaaaagctt
      481 cggaaacacg ggtaacggtt tgattcaatg caaaatcgcg tgaatccaag acgtttggtt
      541 tagaatccgc ccgtgccaaa aataacagtg aggaaaccaa aaacgtcatt tggcggagtt
      601 cctcttggat gttgttcagc caatcggctt gactggcaac ggtttcttcc ggatcgctca
      661 gaaccacttc cagattggtc tggatgatgg ctaacggcgt ccggagctca tgagaggcat
      721 cggccaaaaa atcctgctgt tgccgccagg ctttttgaat gggagcaatc gcacgcccgg
      781 ccatgaataa actgcctagc atcgccagga tgaaaaaaac cactccaaca atgctcaatg
      841 aaaccaccaa agaatgttga atattcttgt cttgtttcag atcttgaaac acgatcagca
      901 tccccgattc cttttgtaac ggcgcccggt aataatgata cttggaatta aaaagatcaa
      961 cgatcccgct ggctttgtcg gccttcaaga tcgattgaat cgctatggtg aggtggagcg
     1021 cgtcccaagg ctcccgaaat gattgaaaca acatattacc cgaaaaatcg gttttgacga
     1081 aaaagaatcc tggtgaagga ggggtttgag tagggcgcgg cggtccagga gggagaacgg
     1141 gaggtttcct gatgccatgt gaaggattgt cgtgcatccc ttcaaaccga gggggcctgg
     1201 catcatcggc cgggaacccc ggaaagactc cggaattaat tcccatcgcc aaccgtttcg
     1261 aaaacacctc ggcatgattg atcattttta tctctaaaat ggcataagcc cctacggtaa
     1321 gcgtaacgaa caaagccgat atgataccca ggttggtcag aatgaattga agccttaatt
     1381 tacgaaacat cggattcccc tttcaggaaa taccctacgc cgcgaaccgt tacgatacaa
     1441 gagggattta gtttcttccg gagataatga atataaaggt cgatattgtt aaaatcggtt
     1501 tcagaaccat acccccaaac tctttcaaaa atcagctcct tcgtaacaac ctggccatgg
     1561 ttgcgcatta acaattcaag taacaaggcc tctttgacac ttaattgaat cacttcggtt
     1621 cccctggtca ccatgcactt cagcggatcc aggatgagtc ctccagcgtt gattgtattt
     1681 cccaccaact cccggttctt tctccgggcc agcgcccgca accgggccaa taattcctcc
     1741 gtggcgaaag gtttaaccag ataatcgtca gccccggcat ctaagccctc aacccgatcc
     1801 tttggcgcgt ctttggcggt cataaagatc accggcatat cgagtccttc tgcgcgaagt
     1861 tccttgataa tcgcaatccc atcttttccc ggcaacatcc gatccaaaat aatcaaatca
     1921 tagtttccat tctgtgctag ctccaaccct ttttcgccgt ctgtggcagt atccacgata
     1981 taaccgcttt tttccaaaag atgggccaat gccttggcga gtttaatttc atcttcgatc
     2041 aatagtagcc tcaaggaaac ctgcaacctc cttttaaaat acccatcatg gtgtattgaa
     2101 cgaacgagat tagccaaaac atcgagcgat taattaggac tatctcactc atcataacgg
     2161 atggttcttt gaatcatctt tatatttcaa aggaaattca aaagttttat tccgattccc
     2221 tgattatcaa agctaaatcc gatacatcat ttcaaaaaac tggaggtctt aagatgaata
     2281 tcgcatctac aacatccggg ttgagcggca gtattttaac cacaggttat aatgacaata
     2341 ccttgaccca attggaaaag gagaaatcgg atcttgaggc acagcttcag aaagaacagg
     2401 caagtcaaac agatgcccaa accaagcagc agaagataga acagctccaa aaccagataa
     2461 accagattga gatccggatt aatcagcaaa aggcggccca acaaaataag gttgcagact
     2521 catttgggga tgattcatcg gctggttccg attcaaccct tcgaacttcg tcaacccaaa
     2581 tcgacataaa aatatgaaca tgatgggcga atccagacat acaaacaaag gtgggatatg
     2641 attccacatc ccaccttctc cttgcatcag gcaaggtctt ttacctttgt ttattcccct
     2701 tcacctcgta ccacccgggg atctcatcat aagtccggta atcatcattt cctttgatac
     2761 gtttgaccgc gaaattcact gtcacacttt ttggatcacc cagcatcttt aatggtatca
     2821 ccgtttcaag aggattataa ctcagtgaag ccgagccaat cggaacggcc tttccctgat
     2881 cgatacgaaa aatatcggcc cggttcaaat tggagaggaa caggactaaa aattgattcc
     2941 cctgatcgcc gttacattga atcgagatat cgaccatcgg atcaacactg ttcgttttag
     3001 ctgctaaata aagatatcct tcatccataa cagcataccc ggcctcaaga tccgtggccc
     3061 cgtcaagcgc atccccaact ttatccactg ccaacggttt gatttggttc caatcgatgg
     3121 tggcccaatc cttgaggtaa tcgatcaaat cttgatgttc ttgggaaata accgcaaacg
     3181 gcgtggcccc atccttgttc tgtagagatt tgtctgcccc cttactgatc aggtatttaa
     3241 ccagctccag atcaccctta ctgcaggctt catgcaaagg ggtccaaccg gaatcatcgc
     3301 cgcggttgac gtccgccccg ttttccacca gtaaccgcac ggtttccaaa gaatgttcat
     3361 tactcttaat cgccgtaagc agtggagttt gaccgccgtt gcaaggcagg ttgatgtttg
     3421 ctcccagagc cagtagatct ttgattgcct gggagctgcc catcatcgcc gcataatgga
     3481 gcacactgcc gaaatccctg acccacccat tgatatcgac gcccttcgcc aacagcagct
     3541 tggcgatctc catttgattt cccaagcggt tcccaacgat aaaatccacg tagagcggcg
     3601 tttctccgtt ttcacttact gctttgggat ttgcgccgtg gtccaataag tattgaatga
     3661 tatcggtggt cgccccccag tcgtgaatcg cgaacatcag cggggtccag ccgcgcaacc
     3721 attgattatc ggggttatcg gcgtacttgc cttcatagcg gaaactttga ttgatatcgg
     3781 cttccttctc gaggagaagt ttcaccgctt ccagataacc atgatgactg ttatgacagg
     3841 cccaggcaag tggtgagtgg ccgtttccca gagtgttcac gttggcgcca tggtcaatga
     3901 ggtattgcat caccgcaa
//
LOCUS       DASVMB010000078         5939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_451910,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000078 DASVMB010000000
VERSION     DASVMB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451910"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VF531_02545"
     CDS             complement(<1..404)
                     /locus_tag="VF531_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEX7712876.1"
                     /translation="MADLILASASPRRAAILKQLGLRFTVAPSSFPEESVTSGSPEQL
                     VTTLALNKAKQVAGAHTPETRVGSLVIGADTVVVLDGEILGKPADLNEAVTMLTRLSG
                     KAHSVFTGLALVSVPESRTRVGYTETRVYFRDL"
     gene            complement(469..723)
                     /locus_tag="VF531_02550"
     CDS             complement(469..723)
                     /locus_tag="VF531_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HEX7712877.1"
                     /translation="MGRSGNAHWLVLVILLLIGFIFGTIMGQILKPYLPFLAVGAPAG
                     MEPQTFHLADAFSLTFGFKVHLSLATIIGIALAYLIYRQI"
     gene            complement(1203..3950)
                     /locus_tag="VF531_02555"
     CDS             complement(1203..3950)
                     /locus_tag="VF531_02555"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HEX7712878.1"
                     /translation="MDQTQWYQLDQHQTAAKLGTDPDHGLTETEVKTRLAQHGFNELA
                     EEAGKSIWQMLLEQFKEFLVILLLVAAIISGFLGEWLDSIVILLIVVLNAFLGVFQEY
                     RAEKSLAALKKLAAPLARVLRDGQVREIPARELVPGDIFFIEAGGFVPADARLFEAVN
                     LRVDEAALTGESVPVEKSADTLHEELLLADRKNLVSMGTVVTYGRAKGIVIKTGMHTE
                     IGQIAKMIQQATPETTPLQEKLAEFGKYLGVLALVVCAIIFVVGWIRGENPVSMFLTA
                     VSLAVAAIPEGLPAIVTIVLALGVQRMAKHRAIIRKLPAVETLGAATVICSDKTGTLT
                     QNQMTVRRAFVTGKMVEVTGEGYQPSGQFLLDGAPQSSPGESLNLLLLCGTLCNDALL
                     IEDDQPDKVIWRIVGDPTEGALVVVAAKAGLEKEALNKSFERVLEIPFDSDRKLMTTI
                     HKGSLPHSRLRQFEQEEPHGLWAITKGAPDIVLSRCTNYLSEDGIWPLVTADKETLLK
                     TNGTLAREALRVLAVAVRRVPEVDQVKLEQVESQLTFIGFWGMIDPPRSEARAAIAEC
                     RHAGIKPVMITGDHRDTAMAVAKELGLLEEGDLVLSGQELNAISDEELKTKVDHIAVY
                     ARVSPEHKVRIVNALREKGQVVAMTGDGVNDAPALKRADIGAAMGITGTDVAKGAADM
                     VLADDNFATIVGAVREGRIIYENIRKAIYFLLSCNIGEIITIFLAIILQYPVPLVAIQ
                     ILWINLVTDGLPALALGMEPAEPGIMNRKPRRKEQGIFSAGMKRTIFIEGFLIGSFTL
                     LAFIIGLNFGGDLSQARTMAFATLSLSQLFHVFNFRSIKESIFKHGTVPNYSLIAAVA
                     LSAGVQLIVMFAPLLQNIFKVVPLDPLHWFYIVVLSAATIPVVEIWKAAFLRKFCREE
                     F"
     gene            complement(4018..4410)
                     /locus_tag="VF531_02560"
     CDS             complement(4018..4410)
                     /locus_tag="VF531_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7712879.1"
                     /translation="MWQEIEITTQNRVEWPDITERVGAIVKQSGVREGMCRIYIPHTT
                     AGITINENADPDVVRDILAGLKRLVPQNGDYAHAEGNSDAHLKASLMGFSCLVPICSG
                     RLALGTWQGIYFCEFDGPRRRHILIDCS"
     gene            complement(4845..5141)
                     /locus_tag="VF531_02565"
     CDS             complement(4845..5141)
                     /locus_tag="VF531_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX7712880.1"
                     /translation="MEFTDKTLVCSDCGAEFVFTAGEQEFYASRGFTNEPRRCKTCRE
                     GRKQQRGDASHPRELHEVVCANCGVTTQVPFKPREDRPVYCRDCFQAMKNGENK"
     gene            complement(5384..5572)
                     /locus_tag="VF531_02570"
     CDS             complement(5384..5572)
                     /locus_tag="VF531_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712881.1"
                     /translation="MNKENIQRMLAGKTILKADLSHGTVKLQFDDGTVFEREKTCEGE
                     IMATLYDGNRQILGSCKI"
     gene            complement(5688..>5939)
                     /locus_tag="VF531_02575"
     CDS             complement(5688..>5939)
                     /locus_tag="VF531_02575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7712882.1"
                     /translation="TKIYWWQERYNSMIINIAEETRTRFIDIRGAFLQNPDYTQYICK
                     DGIHPNEAGHRIIADRILGYIRINYDFLLKEPDWKYVGV"
BASE COUNT         1606 a         1540 c         1314 g         1479 t
ORIGIN      
        1 aggtcgcgga agtaaactcg cgtctcggta tagccaaccc gggtccgaga ttcaggaacc
       61 gatactaagg ccaggccggt gaagacggaa tgggcctttc cgcttaaccg ggtgagcatc
      121 gtcacggctt cattcaaatc agcgggtttt cccagaatct cgccatccaa aaccacaaca
      181 gtatccgcgc cgattactag acttccaacg cgtgtttcgg gtgtgtgtgc acctgccacc
      241 tgttttgcct tatttaatgc cagtgtagta acgagttgtt ccggtgaacc ggacgtaacg
      301 ctctcttccg ggaaggagct gggagccact gtaaatcgca acccaagttg tttgagaatg
      361 gctgcacgcc ttggtgaagc ggaagccaaa attaaatcgg ccattctttc caatcatcca
      421 tcctatcgaa aactctacca ataacgggag aacattgact catagacttt aaatctgcct
      481 gtaaataaga taggccaagg caattccgat gattgtcgct aaactcaggt gaaccttaaa
      541 cccaaaagtc aatgagaacg catcggccag atgaaacgtt tgcggttcca tgccggccgg
      601 agcgcctacc gccaagaaag gcagataggg ttttaagatc tgtcccataa tcgtgccaaa
      661 gatgaaaccg attaataaga gaatcaccaa taccagccaa tgcgcatttc cggaacggcc
      721 caaccgtcat tcacccgctt tgcccttaaa aaaaaggttc tatagagtcc gattgacttg
      781 agaaataccc ggggccgaac gaaaaacaac cgcaggccca cattcaagcg attagtgatt
      841 ccacatccgg tttctaaaat ccttttcgat atcgaaaagc ccgttaaatt tatctggaat
      901 ccaagcaaaa aactgacaac acgaatatgg acaacatggc atcggtacaa aaagtaaaac
      961 gacccgatgg gtcgtttagt ttccatcccg ggatttggat ttaattcctg tgacggtttg
     1021 gtgagcccgt tgccaacaaa cgttccgttt accggactct acggtaaaga ttccaggaac
     1081 cattatatcc gatccatgac gaatattcta tacctggatt gcttactttt aaaaaaatct
     1141 gaatgcaata cgaatacttt aactgcgggg gcagtctttg gtgaacgacc gccccgcaca
     1201 gattaaaatt cttcccggca aaatttacgt aaaaaggccg ctttccagat ttcaactacc
     1261 ggaatcgttg ctgcggaaag caccacgata taaaaccagt gtagcggatc aaggggtacc
     1321 actttaaaaa tgttttgaag caaaggagca aacatgacga tcaattgaac tcccgctgaa
     1381 agcgccacgg ccgcaatcag tgagtaattg gggactgtgc catgcttaaa gatggattcc
     1441 ttgatcgaac ggaagttgaa tacatggaac agctgcgaca agctcaaggt ggcaaaggcc
     1501 atggttcggg cttgagaaag atcgccgccg aagttcagcc cgatgataaa tgccagtaat
     1561 gtaaaggaac cgatcaaaaa gccctcgatg aatatggttc gcttcattcc ggcagagaag
     1621 ataccttgct ctttgcgacg gggcttccgg ttcatgatgc ccggttccgc gggttccatt
     1681 cccaaagcca aggcaggcag accgtctgta accaggttaa tccagagaat ctggatggca
     1741 acgagcggta ccgggtactg caagatgatg gccaaaaaaa tcgtaataat ctccccaata
     1801 ttgcaggaaa gcaggaaata aatggctttt cttatatttt catagataat ccggccttca
     1861 cgaaccgcgc caacaatcgt cgcaaagtta tcatccgcca agaccatatc tgctgcgcct
     1921 ttggccacgt cagttcctgt aatacccatc gccgctccga tatcggcccg ttttaaagcc
     1981 ggtgcatcat tcacaccatc gccagtcatc gcaacgactt gtcctttctc acgcaacgca
     2041 tttacaatac gcactttgtg ttccggggaa accctggcgt ataccgcgat atggtcgacc
     2101 ttggttttga gctcctcatc acttatggca tttaattctt ggccacttaa gaccagatcg
     2161 ccctcttcca agagtcccaa ctctttcgcc acggccattg cagtgtcgcg atgatcaccg
     2221 gtgatcatca ccggcttgat tccggcgtga cggcattcgg ctatggctgc acgggcctcg
     2281 gatcggggag gatcgatcat gccccagaac ccgataaaag tcaattggga ttcaacctgt
     2341 tccagcttaa cctggtctac ttccggaacc cggcggacag ccaccgccaa tactcgtaat
     2401 gcttcccggg ccagggttcc attggttttt aacagagttt ctttgtccgc agtgactaac
     2461 ggccaaattc catcctcgga cagataattt gtacaacggc ttaaaacaat atccggagcg
     2521 cctttggtga tagcccaaag accgtggggt tcctcctgtt caaattgacg cagtcgactg
     2581 tgaggcagac tccccttatg gatggtggtc attaacttcc gatcggaatc aaaaggaatc
     2641 tctagcaccc gttcaaaact tttattcaag gcttcttttt ccaacccggc tttggcagcc
     2701 acaacaacta aggcgccctc ggtgggatcc cccacgattc gccagattac tttatccggc
     2761 tgatcgtctt caattaataa cgcatcattg cacaatgttc cacaaagtaa caacaagtta
     2821 agggactcac cgggagacga ttgaggagcc ccgtccagta gaaattgacc gcttggttga
     2881 taaccttctc cggtcacttc caccatcttt ccggttacaa aagcccggcg cacggtcatc
     2941 tgattctgag tcagggttcc ggtcttgtcg gaacagatga cggttgctgc acctaaggtc
     3001 tcaacagccg gtaactttct gataatcgcc cggtgtttgg ccatgcgttg aacacccaag
     3061 gccaggacga tagttacaat tgccggcaga ccttcgggaa tggctgctac cgccaagctg
     3121 accgcagtca gaaacatcga taccggattt tcaccccgga tccatccgac gacaaaaata
     3181 atcgcgcaca ctaccaaagc cagaactcca agatatttcc cgaattccgc taatttttcc
     3241 tgaagcgggg ttgtctccgg agtcgcctgt tgaatcatct tggcgatctg accaatctcg
     3301 gtatgcattc cggttttgat tacgattccc ttagcccggc cgtaagtaac aactgtgccc
     3361 atggagacca agtttttcct atcggcgagc agcagttcct catgaagggt atccgcggac
     3421 ttttccaccg ggaccgattc accggtcagg gcagcctcat cgacacgaag attgacggct
     3481 tcaaagagtc tggcatcggc gggcacaaat cctccggcct caataaaaaa gatatcaccg
     3541 ggcaccaatt cccgggcggg tatctcccga acctgcccgt cccttaacac ccgcgcgagt
     3601 ggagcagcca gtttttttaa ggcggccaga gatttttcgg cgcgatattc ttgaaatacc
     3661 cctaaaaagg cattcaaaac cacaattaat aaaatgacga tggaatcaag ccattctcct
     3721 aaaaaacccg agatgatcgc ggccacgagt aataaaatca ccaaaaattc cttaaattgt
     3781 tccagcagca tctgccagat ggattttcct gcttcctccg ccagttcgtt aaatccatgt
     3841 tgagccagac gggttttgac ttcagtttcg gtcaaaccgt gatctggatc ggttcctaac
     3901 ttggcggctg tttggtgttg atcaagttgg taccactggg tttgatccat tcctgtcact
     3961 ccctatgcag tgtttgccct ttttttttca taaattccgg atgctaacca aagtatttta
     4021 actgcaatcg attaaaatat gccgtcgccg gggaccatca aattcgcaga aataaatccc
     4081 ctgccaggtt cctaaggcta accggccgct acaaatgggg accaaacatg aaaaccccat
     4141 cagacttgcc ttaagatgag catcagagtt accctcagcg tgggcgtaat cgccattttg
     4201 gggaaccaac cgtttaagac cggccagaat atcccgtacc acatcgggat cggcattttc
     4261 gttgatggtg atcccggcgg tggtgtgagg gatataaatc cggcacattc cttcccgcac
     4321 tccgctttgt ttgacaattg ccccaacccg ttccgtaata tcaggccatt cgacccggtt
     4381 ttgagtagtg atttcaattt cctgccagat gtgtgttcac ctccttagat aggcatgagg
     4441 cgcgaggcaa cagtaaagag taaaaaggca cgctgcctgt tgcctattat caactcaccc
     4501 ccgtcccctc ttttcgaaaa agagaggggt gtaggaatga actgatagct ataggatgac
     4561 gcggggtgag ttgccgcgcg ccgacagggc acgacacctc acgccccacg ccttaatacc
     4621 cctattgcct attgccttta gtatgccctc cggtcgttga ttccaggaaa taaaaaaacc
     4681 tttaacattg agaatgttaa aggtctggct acttgaattc aagcggaaag gccaggtatt
     4741 cggcataccg agaatgttgg ccattcctgc ggtgtaaaaa caccgccagc tactcccctc
     4801 agagatacaa atccattcat aaaagaagga ttaagggcag agatttattt attttcacca
     4861 ttcttcatag cctggaagca atcacggcaa taaaccggac gatcttcacg aggtttgaaa
     4921 ggaacctgag tggttacacc gcaattcgcg caaaccactt catggagttc ccggggatgg
     4981 gatgcatcac cgcgttgttg tttccgacct tcccggcagg ttttgcagcg acgtggttca
     5041 ttggtaaaac cacggctggc gtaaaactct tgttcaccgg ctgtgaaaac aaactcagcg
     5101 ccgcaatcag aacacactaa agtcttgtca gtaaattcca cgaaaatccc ctcgcttaac
     5161 agtaggattt tacccgaccg cctggtgtat gatatcgaaa caaacgattc cgctccagac
     5221 gattaggtat tgaaaccgtg atcattattt taccgaatgt aacacaaaac ctctctaatt
     5281 cccatgaacg aaaaaaaatt tacaaattta ttataacatc ggaactgcga aaagacaagc
     5341 tatttcatga atagcacatt caaaccctct acaaactggg aaattagatc ttgcagctac
     5401 ccaaaatctg gcgattacca tcgtataaag tcgccataat ctcgccctca caagtctttt
     5461 cccgttcaaa gacggttccg tcgtcaaatt gtaatttaac cgttccgtgg cttagatcgg
     5521 ccttgagaat ggttttaccg gctaacatcc tttggatatt ttctttattc attcatcttc
     5581 accttggata agatattcgt tttagcctcc aattatcctg ccggcaaagt aataatcaaa
     5641 atttttttaa tcaccgtaaa ttccacgggt gagttaagtg tctgttttta cacgccgacg
     5701 tatttccaat ccggttcttt cagaagaaaa tcgtaattga tccggatata gcctaaaatt
     5761 cgatcggcga tgatccggtg accggcctcg ttgggatgaa tcccgtcctt acaaatatat
     5821 tgcgtataat cgggattttg tagaaaagct ccgcggatat cgataaaacg ggtccgggtc
     5881 tcctcggcaa tattgatgat catcgagttg taacgctcct gccaccaata aatcttggt
//
LOCUS       DASVMB010000079         5823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_377165,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000079 DASVMB010000000
VERSION     DASVMB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377165"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF531_02580"
     CDS             complement(<1..228)
                     /locus_tag="VF531_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX7712883.1"
                     /translation="MDVKDTEVRAPAGKSGPKYAAALRYKPGKDQAPVVVASGQGHIA
                     ETIIKLANDAGVPNHVEPALAKILSRLEPGTP"
     gene            complement(219..1691)
                     /locus_tag="VF531_02585"
     CDS             complement(219..1691)
                     /locus_tag="VF531_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712884.1"
                     /translation="MKINTSNPYSILTDSAQTLGELNLKEGQRIIGKIIRLAGDQALL
                     EMAGRPLTAKVEGVQNLNPGSAYTFEVATDDQNRVVLRIANNQNTAAPETGAQPSGSQ
                     SSNSSQPELAVQKAIINALENAGLPVTNENIASVSGSLREFEVKYQQPLPPQVITFII
                     SQKWPINPGTILAAWIHQDPGVRNLLWNLLQRAALQKTVAGGNPDDGMHEWVFDPRTD
                     TTAASLTAKLQSYSGMQLSRLLEQIFGLDQAFPDQNPDPQTSQPDRELQPGKTDITDS
                     RGQGKNQVQPSRTRPPHPGQQSIGSPIRERATGIKNIPDDLSSFHMIKDDPAQTARIS
                     NQADTNSKASAVQGDNQAPKFLALLEQNQKLENVLHQEPGIYAAIPLLVRDPQNHLHE
                     AVIQWEGRKSGHSENFYEQLIQLAVPTDNMGEVLLRVRIGANGANIHMKVGSEAVRRF
                     FMQNAAELRSAISESEAMIQVSVRESVTSEHSTMGVDLWM"
     gene            complement(1663..2373)
                     /locus_tag="VF531_02590"
     CDS             complement(1663..2373)
                     /locus_tag="VF531_02590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7712885.1"
                     /translation="MRTVHKVLPKLSWDERLTYEQHCWQNGSTRVAGLDEAGRGPLAG
                     PVVAAVFLITPDFSLPGLNDSKQLTALQRQRFYQVLTSGAWEYATGLVEPGEIDRINI
                     YQASRIAMSKALQSLKVRPDFLLVDALVIPDTSVKQQAIIHGDALSVAIAAASVIAKV
                     TRDKLMEEYDRIYPGYGFAQHKGYPTAAHYEALQKLGPSPIHRLSFRLTKAPREDSLE
                     LFETEGDMTVEDQHLKSV"
     gene            2528..3574
                     /locus_tag="VF531_02595"
     CDS             2528..3574
                     /locus_tag="VF531_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX7712886.1"
                     /translation="MKTTQLPVKDWFFKGLSLVLIFTLLSTGYLLFNSTSPNLWSGFG
                     KINLGAFGLACLALVGSWLIEVCRIILIAGGLGEKLSFMNVLGINLAATFSGNVTPFY
                     SGGIPTQIYLLCRNGISPGKSSAIVTLRVIISTLIFTLVSPFLLLFYHAKFSVGIIRQ
                     VTTVAIPLSVLASACLILFIVKPKLASGIFTRILKLLEFIGLKKKIQPVMEKFLNELE
                     SFHDAIKEFRKGINFYLTFICSFLYWVCFFAIAPFLMAAFGMDISQIFWQSILFQFIL
                     VFIISYIPIPSGSGIMEFGFYSVFVFIPMQLRAIFILVWRFLSYHVATFIGGIILLRT
                     LNRSNDSDVEATPN"
     gene            complement(3531..4361)
                     /gene="ylqF"
                     /locus_tag="VF531_02600"
     CDS             complement(3531..4361)
                     /gene="ylqF"
                     /locus_tag="VF531_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEX7712887.1"
                     /translation="MQPTIHWYPGHMAKAKRELTELIRYLDLIIEVRDARIPIASHNS
                     DLDRFLERRPLFVVLNKCDLVSPKDIAAWSDWFVKQGLSMVTMNGRSGEGIAALLRML
                     TRDSVPGRSVSKRIGVVGIPNVGKSSVLNRLVGGKNARTGNIPGITRGKQWVKRGKLE
                     ILDTPGLLPPKIQNPEDGWKLALIGALPEEVIPIYDLALFILNQFGQSLFNWEDAGLQ
                     QADGEIRLEWFAGKRQFIKKGGGPDLNRAAVTIVKEFRDGRLGPISLESPPHPNHSNG
                     "
     gene            complement(4696..5586)
                     /gene="xerD"
                     /locus_tag="VF531_02605"
     CDS             complement(4696..5586)
                     /gene="xerD"
                     /locus_tag="VF531_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX7712888.1"
                     /translation="MQNNLQDYLNYLTVERGLASNTLESYSRDLRQYLNYLDERKHLL
                     LSDTNQATVIGYLLQLQAHGKATATLSRSLAAIKSYYHFMARERMIERDPTLNLDAPK
                     QEKRLPRVLSVADVERLLEQPNLKLPVGVRDRAMLEVLYATGLRVSELVSLKLNDVNL
                     ETGYIKCLGKGSKERIIPLGSMATKYLKLYQEHARKFLAASLHEDTLFLNHHGKGLTR
                     QGFWKIIKKYAENLEIEVDITPHTLRHSFATHLLENGADLRSVQEMLGHADISTTQIY
                     THLTKDKIREVYDQAHPRAK"
BASE COUNT         1370 a         1479 c         1389 g         1585 t
ORIGIN      
        1 cggtgttccc ggttccagac gagagagtat tttggcaagt gccggttcca catggttggg
       61 aacaccggca tcattcgcca gtttaataat agtctcggcg atatggcctt gcccggaggc
      121 caccactacc ggtgcttggt ccttaccagg tttatagcgc aacgcagccg catatttcgg
      181 tcccgatttc cccgccgggg cgcgtacctc agtgtccttt acatccatag gtccactccc
      241 atggtagaat gctcggaagt tacactctct cttacactta cctggatcat cgcttccgat
      301 tcggaaatgg ctgatcgtaa ctccgcggca ttttgcataa aaaatctccg gaccgcttcc
      361 gaaccaacct tcatatgaat attggcgcca tttgctccga ttcggacccg taaaagaacc
      421 tctcccatat tatcggtggg aactgcaagt tgaatgagtt gttcgtaaaa attttccgaa
      481 tggccggact ttcgcccttc ccattgaatg accgcttcat gaagatggtt ctgaggatcc
      541 cgaactagca acggtatcgc cgcatatatc ccgggttcct gatgcaagac attctccagc
      601 ttctggttct gttcgagcaa ggcgagaaac ttcggtgcct ggttatctcc ttggacggca
      661 gacgctttag aattcgtatc cgcttgatta gaaattctag cggtttgagc aggatcatct
      721 tttatcatgt gaaacgacga aagatcatcc gggatgttct tgataccggt agctctctcc
      781 cggatggggc taccgatgga ttgctgaccc gggtgaggcg ggcgcgtgcg ggacggttgc
      841 acctggttct tcccctgtcc ccgggaatcc gtgatatctg ttttacccgg ttggagctcc
      901 cggtccggtt gagatgtttg gggatcggga ttttggtccg gaaaggcctg atccaaaccg
      961 aaaatctgct ccagtaatcg tgaaagttgc atacctgagt atgactggag cttggccgtc
     1021 aaggaagcgg cagtggtatc ggtacgcgga tcaaacaccc attcatgcat cccatcatcg
     1081 ggattcccgc cggcaacggt tttctgaagg gccgctcttt gtagtaaatt ccagagcaga
     1141 ttacgaactc cgggatcctg atgaatccaa gccgccagaa tcgtcccggg attaattggc
     1201 catttttgag agatgataaa tgtaattact tgaggtggca agggttgttg atacttgacc
     1261 tcaaattccc ggagactccc ggatacactg gcgatattct cgttcgtcac cgggagtccc
     1321 gcattttcaa gcgcattaat aatcgccttt tgaacggcaa gttcaggttg ggagctattt
     1381 gatgactgtg acccggaggg ttgggcaccc gtctcgggcg ccgccgtatt ttgattattg
     1441 gcaatcctta acacgacgcg gttttgatca tccgtggcga cctcaaaggt ataggctgaa
     1501 cccggattca ggttttgaac tccttccacc tttgcggtga gcggtctacc cgccatctcc
     1561 agtaaggcct gatcacccgc caaacgaata atcttgccga taatcctttg tccttcttta
     1621 agattcaatt ccccgagcgt ctgcgcggaa tcggtaagga tactatacgg atttgaggtg
     1681 ttgatcttca actgtcatat caccttccgt ttcaaacagc tccaaactgt cttcccgtgg
     1741 tgccttggtt aatcgaaacg ataagcggtg tatgggcgaa gggcctaatt tttgcaaggc
     1801 ttcgtaatga gccgcagtcg gatatccttt gtgttgggca aatccataac cgggatagat
     1861 ccgatcatac tcctccatca gcttgtccct tgtaacttta gcgattaccg aggctgcggc
     1921 gattgctacg gagagcgcgt ctccgtgaat gatcgcctgt tgcttgacgg aagtatcagg
     1981 gatcaccagc gcatccacaa gcaagaaatc cggccgaacc ttcaaactct gtagcgcctt
     2041 ggacatggct atccgtgagg cctgatagat gttgatccga tcgatttcgc cgggttccac
     2101 cagcccggta gcgtactccc aggcgccaga agtcaaaacc tgatagaatc tctggcgttg
     2161 taacgccgtc agttgtttgg aatcgttcag acccggcaag gaaaaatccg gtgtaatcaa
     2221 aaagaccgca gcaaccaccg gaccagccaa ggggccgcgg ccggcttcat ccaaaccggc
     2281 aactctcgtg gacccgtttt gccaacaatg ctgttcgtaa gtgagccttt catcccagct
     2341 caatttgggt aacaccttgt ggactgttct cattttggtt gcctctggta cgcgggtgtg
     2401 cgtcgcggcg cacacgcaca atttttcgga agattatagc aatgaattca acttttactt
     2461 gcaggatcta tttaaccaaa aaagaatttc tatatataca ttttgactag gtaaaggaga
     2521 ttcagccgtg aaaacaacgc aacttccggt gaaagattgg ttttttaaag gccttagcct
     2581 ggtactgatc ttcaccttgc tgagcacggg ttatctcttg tttaattcga catcccccaa
     2641 tctctggtcc ggcttcggta aaatcaacct cggagccttc ggcctcgcct gcctggcact
     2701 ggtcggctcc tggttgattg aggtctgccg gatcattttg attgccggag gtttgggaga
     2761 aaaactatct ttcatgaatg tcctggggat caatctggcc gcaacctttt ccggaaatgt
     2821 aacgcctttt tattccggcg ggatccctac tcagatctac ctgttatgcc ggaacgggat
     2881 tagccctggt aaatccagcg ccattgtgac cctgcgggtg atcatttcaa cgctcatctt
     2941 tacactggtg tcaccgttcc tactcctatt ttaccatgct aaattctcag tcgggatcat
     3001 ccgtcaggtg acgaccgtcg ccattccgct ttctgtattg gcctcagcct gcctgattct
     3061 atttatcgta aaacccaaac tggccagcgg tatcttcacg cgaatcctta aattgctaga
     3121 gtttatcggg ttaaaaaaga aaattcaacc ggtgatggaa aagtttctaa acgaattgga
     3181 atcgtttcat gatgcaatca aggaattccg gaaaggaatc aatttctatc tgacctttat
     3241 ctgcagcttt ttatattggg tctgtttttt cgcgattgcg ccgtttttga tggcggcttt
     3301 cgggatggat atcagccaaa tcttttggca aagcatttta ttccagttca ttctggtctt
     3361 tattatttca tatataccga tccccagcgg tagcggaatc atggaattcg gattttactc
     3421 ggtattcgtt ttcatcccga tgcaactgcg ggcgatcttc attctggttt ggcgattcct
     3481 cagttatcac gttgccacct ttataggagg catcatctta ctaaggacac tcaaccgttc
     3541 gaatgattcg gatgtggagg cgactccaaa ctaatcgggc ctaatcggcc atcacggaat
     3601 tccttcacta tggtcacggc ggcccggttc aaatccggtc cgccgccttt cttgataaac
     3661 tgcctttttc ccgcaaacca ttccaaacgt atctcaccgt cggcctgctg taacccggca
     3721 tcttcccaat tgaacaaaga ctgcccgaac tgattcagga taaaaagcgc caaatcgtat
     3781 atgggaatga cttcctcagg aagtgcgccg atcaaggcca gtttccaacc gtcctcggga
     3841 ttttgaatct tcggcggcaa aagtccgggg gtatccaaaa tctcaagctt tccccgcttg
     3901 acccattgct ttccccgggt gatcccgggt atattaccgg ttcgggcatt ttttccacct
     3961 accaagcggt ttaacacgga ggatttcccc acatttggta ttcccaccac accgattcgt
     4021 ttggaaacgg aacggccggg taccgaatcc cgggtcaaca ttcgcaagag ggcggcgata
     4081 ccctctccgc ttctcccgtt catggtcacc atacttaacc cctgctttac aaaccaatcg
     4141 gaccacgccg caatatcctt cggggatacc agatcacact tgttcaatac aacaaaaagc
     4201 ggccggcgtt ccaaaaaacg atccagatcg ctattatggc tggcgatggg gatccgggca
     4261 tcccggacct cgatgattaa atccaaatag cgtattaatt cggtgagttc ccgtttggcc
     4321 ttggccatat gtccgggata ccaatgtatt gttggttgca tcaatccgcc gcttctatgt
     4381 taaagtttag gcacttgcct gttccctgct ccctgttgcc tttataaata atacttaaat
     4441 tataccaaat ttcccggtcc tggtatactc catttcgcaa cgaatacaaa aaactccgcc
     4501 ggtttggatg acggagaaat cgagagtctt attggcaact cacccccgac ccctctcttt
     4561 tcagaaagag aggggcgtag ggtcattctg aaagctaata gaaaacgcgg ggtgagttgc
     4621 cgcgcgtccc gcaggacgcg atgcctcgcg cctgttgcct attgcctgtt tccgcgcgcc
     4681 ctgctaggtg tgagtctatt tggcacgggg atgggcttga tcatagacct cccggatctt
     4741 atccttggta agatgggtat aaatctgggt ggtggagata tcggcgtggc ccaacatctc
     4801 ttggacggaa cggagatcag ccccgttttc caacaaatga gtggcaaaag aatgccgtag
     4861 cgtatggggg gtaatatcta cctcgatctc aaggttttct gcatatttct tgatgatttt
     4921 ccaaaatcct tgtcgggtca agcctttacc gtgatgattc aagaacaggg tatcttcatg
     4981 caaactggca gccaaaaatt tccgggcatg ttcctgatac agcttaagat acttggtggc
     5041 cattgaacca agagggatga tccgttcttt ggaccctttt ccgaggcatt tgatataacc
     5101 ggtttccagg ttgacgtcgt taagttttaa agaaaccagt tccgaaactc gaagcccggt
     5161 ggcatataga acctctaaca tggctcgatc ccggacaccc acgggtaact tcaaattggg
     5221 ttgttctaac agacgttcca catcagccac tgacagaacc ctcggtaacc ttttctcctg
     5281 tttcggggca tccagattaa gggtgggatc gcgttcgatc attctttctc tggccatgaa
     5341 atgataatag gatttaattg ccgccagact ccgcgacaag gtcgctgtgg ccttgccatg
     5401 cgcttgaagc tgtagcaaat agccgatgac agtagcttga ttggtatcgg ataacaatag
     5461 atgctttctt tcatcaagat agttcaggta ttgccggaga tccctgctgt aagattccag
     5521 tgtattactg gccaagccac gctcgaccgt gaggtaattt agataatcct ggagattgtt
     5581 ttgcatttcc ggtcaactcc tttagttaac tttcggaacg caatacaatt tcgacagttt
     5641 tacggttaac tccttttttc gacagtaagg aaagatttcc atcgaggcgg ctcaaacggc
     5701 gacaataggc actttgaacc ggattcgccc gtggagatgg aaaccagttc aaacaataaa
     5761 tggtaaataa ttaaataaga tgggaaagcc aagaggtaaa tgcaggtgtt acataaccct
     5821 gga
//
LOCUS       DASVMB010000080         4301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_377352,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000080 DASVMB010000000
VERSION     DASVMB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377352"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF531_02610"
     CDS             complement(<1..373)
                     /locus_tag="VF531_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809728.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HEX7712889.1"
                     /translation="MREKRAKMAGLKNQRLDETEFAGLRREVLSTWPTGAAIDLKEAV
                     AYQKQLPSGKRFARALEDAARRGATLIQPRAGVALVDDQIALLKHLETSGGADLLPTT
                     VDSYTRQNRYREAQKGIDESTV"
     gene            complement(404..847)
                     /gene="glmS"
                     /locus_tag="VF531_02615"
     CDS             complement(404..847)
                     /gene="glmS"
                     /locus_tag="VF531_02615"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HEX7712890.1"
                     /translation="MKTPTLVLGVIGMDVHAVGNKILEYAFENAGFHVVNLGVMVSQE
                     EFVHAAIETAADVVLVSSLYGHGELDCRGLREKCIEAGIGGILLYVGGNLVVGKAPFS
                     EVERTFKAMGFDRVYPPGTLPETAIRDLQADLHWEGPRISLDSLL"
     gene            complement(849..1850)
                     /locus_tag="VF531_02620"
     CDS             complement(849..1850)
                     /locus_tag="VF531_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7712891.1"
                     /translation="MKLQIEKEAGLPLYLQIKAQIKQQIERGILAPGEQLPTERELAL
                     DLGVSRNTVSQAFAELSEEGVIEVGQGKGTFVTEAASAHPLARRLAEGHKEKALRLID
                     LAIDECLDLGFSLADFTALATVRAREREDSLLKTRVLFIDCNQEQLVNFSREFRELGM
                     LEVIPLLLSDFLEGGLRIGKLLSQVDLVVTTTTHQEIVAEKLKDLGTELELVPVAAQP
                     RLESLIRISRLSGDRSIGLVCLSREFPEIVRRTLAKLNMYDLSLDYTTTKDPEELGQF
                     IENHDLILVYADRYREVKALAGEKEIISYLHGLDRGSVTLVRRAVERIWQQKQKEGR"
     gene            complement(2164..3243)
                     /locus_tag="VF531_02625"
     CDS             complement(2164..3243)
                     /locus_tag="VF531_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7712892.1"
                     /translation="MRFKGTAVVICMAAAMVLGIILPRNFARVGVSSGVASGKTAQET
                     SAGTAQGNGDVQLNELGRIMILEYHLIGYPEAQWRRTPENFKKDLDMLYENGYYPVPL
                     RDLINRRMKVPAGKTPFVLTFDDSSQGQFRFLKKGSQLEIDPESAVGIMEAFKKNHPN
                     FPLTATFYVLPAIDPGLRLFGQEEYIQEKLQFLVQHGYEIGNHSYWHQNLGKTDDAGV
                     QKQLALAVKAIQSYVPGYQVHSLALPLGVHAKNPALERDGEYQGTPYHNDSILLVGSG
                     TAPSPYSADFNPFRLERIQAGDTPWGPKAYIAMFKKDPSLRYVSDGNNAILSAPMALR
                     GKLAKQFNGIFRPKTTPVSLQPVKE"
     gene            3529..4038
                     /locus_tag="VF531_02630"
     CDS             3529..4038
                     /locus_tag="VF531_02630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7712893.1"
                     /translation="MNDLRFVPATPEHIPELLAIYNYYIEKSTATFHLDPVNSEEMAE
                     ILFYTNPRFQAFVISDSSAKICGYCILARFKKREAYDTTAEVTVYLAPEFTGKGIGSL
                     AVKFIEEKARERNFHSLIAVICGENEASIRLFKGLGYVQCALYKEVGKKFGRFLDIVC
                     YQKIIGNRE"
BASE COUNT         1055 a         1156 c         1009 g         1081 t
ORIGIN      
        1 ccacggtgct ctcgtcgatc cctttttggg cttcccggta acggttttgc cgagtgtaac
       61 tgtcaaccgt agtcggcagt aaatcggcac ctccgcttgt ctccaaatgt tttaaaaggg
      121 cgatctggtc atcaaccaac gccacaccag ccctgggctg aattaaggtt gcgcctcgtc
      181 gcgccgcatc ctcaagcgcc cgggcaaacc gttttccgga cggcagctgt ttttgatagg
      241 cgaccgcttc cttgagatca atagccgctc cggtcggcca ggttgataaa acctcccggc
      301 gcaatccggc aaattccgtt tcatcaagtc gttggttttt gagtcccgcc atctttgcgc
      361 gcttttcgcg caccacctcg ctattcccgg caataacggt ctattaaagt aagctatcca
      421 ggctgatccg gggaccttcc caatgcagat ccgcctgaag atcccggatg gcggtctctg
      481 gaagggtccc gggcggatac acccgatcga aacccattgc tttgaaagtt cgttcaacct
      541 cactgaaagg agccttcccc accaccagat ttcccccgac atacagcaag attcccccga
      601 ttccggcctc gatacatttt tcacgcagac cgcggcagtc cagttcacca tgaccgtata
      661 gcgaagagac gagaactaca tcggcagccg tttcaatcgc cgcgtgaaca aactcttcct
      721 gggaaaccat gacccccaga ttcaccacat gaaatccggc gttctcaaag gcgtattcca
      781 aaatcttatt ccccacggca tggacatcca tgccgataac tcccaacacc agggtaggag
      841 tcttcacgtt aacgcccttc cttctgcttt tgttgccaga tccgctctac ggccctacgt
      901 accagggtca ccgaacctct atccaacccg tgcagatatg aaatgatttc cttctctccg
      961 gccagtgcct tcacttcgcg gtaacgatcg gcgtaaacca atattagatc atggttttca
     1021 atgaattgtc ccagctcctc cggatccttg gtggtggtat aatccaagct caggtcatac
     1081 atgtttaatt tggccagggt acgccggaca atttccggga attcccgact gagacaaacg
     1141 agtccgatag aacgatctcc cgataaccgg gagatgcgaa tcaaactttc cagacgcggt
     1201 tgggccgcaa ccggcaccaa ttccaactcg gtccctaaat ctttcagttt ttccgctacg
     1261 atttcctgat gagtggttgt ggtgacaacc aggtcaacct gggacaaaag cttcccaatc
     1321 cgaagaccgc cttctaaaaa atcactcaac agcaacggaa tgacctcaag catacccaac
     1381 tccctaaact cacggctgaa gttaaccaac tgttcctgat tgcagtcgat gaagagcacc
     1441 cgggtcttca gaagtgagtc ttcccgttcc cttgcccgaa ccgtggctaa ggctgtaaaa
     1501 tccgccagcg aaaagccaag atccagacat tcgtcgatgg ccaggtcaat cagacgcagg
     1561 gccttctctt tgtgtccctc cgcgagcctg cgggcaagcg gatgggccga agcggcttca
     1621 gttacaaaag ttcccttgcc ttgaccgact tcaatgactc cttcctccga aagttcggca
     1681 aaagcttggc tgaccgtgtt ccggctcacc ccaagatcca aggcgagctc ccgctcggtg
     1741 ggcagttgct cgccgggagc cagaattccc cgctcaatct gctgtttaat ctgggcttta
     1801 atctgtaagt aaagaggtaa tccggcctct ttctcaattt gtaacttcat cttcgatcac
     1861 cccaaaggat cattggatca atcaatagtt ccattttgat catacaccca aaaaaaattt
     1921 gatgcaagaa aaatctggca gtccggttcc agtccaatag ccagaaagat tgaggattcc
     1981 ccggccggtg aaggactccg ccctcggcag atatccctat catctccaaa gtgcggggga
     2041 ggatacagga agataaccat gaccgttggt tgacctaggg cgcccagata aaaaaggacc
     2101 cgtgattcaa gtagcggaga atcacgggtc cttatactta cctaccgagg cttagggtaa
     2161 tatttattct ttgaccggtt gcagcgaaac cggagtggtt ttcggtctga agattccgtt
     2221 aaattgcttg gcaagtttcc cccggagtgc catgggtgcc gaaaggatgg cgttgttccc
     2281 gtcactgaca taacgcagtg agggatcctt tttaaacatt gcgatataag ccttgggacc
     2341 ccagggtgtg tctccggctt gaatcctctc gaggcggaac gggttgaaat ccgccgaata
     2401 aggcgaggga gctgtcccgg aaccgaccag caagatggag tcgttatggt aaggggttcc
     2461 ctgatactct ccatcccgtt ccaaggcggg gtttttggcg tgcaccccca gcggcagcgc
     2521 cagggagtgt acctgataac cggggacata cgattggatc gccttcaccg ccagcgccag
     2581 ttgtttttga actccggcat catctgtctt gcctaaattc tgatgccaat aactgtggtt
     2641 acctatctcg taaccatgtt gcaccagaaa ttgcagtttt tcttgaatat actcttcctg
     2701 accaaaaagg cgtaaacccg ggtcgattgc aggtaaaacg tagaacgtcg cagtcaacgg
     2761 gaaattggga tgattttttt tgaatgcctc catgatcccc accgcggact ccgggtcaat
     2821 ctcaagctga ctgccttttt tcagaaagcg gaattgtccc tggcttgaat cgtcaaaagt
     2881 caggacgaac ggtgttttac ctgcaggcac cttcattctc cggttgatca aatcacgaag
     2941 gggcaccggg taatagccgt tttcgtacaa catgtccaaa tcctttttga aattctcagg
     3001 tgtccgccgc cattgagcct ccggataacc aatcaggtga tactctaaga tcataatccg
     3061 tcctaattcg ttcaactgaa catccccgtt cccctgcgct gtccctgccg aagtttcttg
     3121 agcggtcttc cctgaggcta ccccggaact aacccccacc cgagcgaaat ttcgcggcag
     3181 tatgatgcct aaaaccattg ccgctgccat acaaattacc actgccgtcc ctttgaaacg
     3241 cacctgtaat tcctccgtta gttttattgc ccgaaatgta cctggcatga accggtttta
     3301 tattatccat tcgggcttga atgaaaatca gattgagttt tcaaggatct ttcggctttt
     3361 cctgcatata atcgctggaa gaccacccgt catctgcggc tagaaatacg gtttcaattt
     3421 tcccttccag cagccgcttg aaattgtgac ctcctttgat ataattagca tggatcggat
     3481 ggaattaata tctaatcata ctcaaaatga aagggcgcca aataaaccat gaatgatttg
     3541 cgttttgtac cggcaacccc ggaacatata ccggagttgc tcgcgattta taattattac
     3601 attgaaaaat ccacagccac gtttcatctg gacccggtta attccgagga gatggcggag
     3661 atcctcttct acaccaatcc gcgttttcag gctttcgtga tctcggactc gagcgccaag
     3721 atctgcggtt actgcatttt ggcccgtttt aaaaagcggg aggcttatga taccaccgcc
     3781 gaggtcaccg tttacctggc cccggagttt accggaaaag gtatcggcag tttggcggta
     3841 aagtttatag aggaaaaggc cagggagaga aattttcatt ccctaatcgc ggtgatctgc
     3901 ggcgaaaacg aggccagtat ccgcctattt aagggcctgg gttatgtaca atgcgccctg
     3961 tataaagagg ttggcaaaaa attcggccgt tttctggata ttgtttgcta tcagaagatc
     4021 ataggcaata gggaataggg aacaggcgtg aggcatgagg cgcgagatta atgagaatag
     4081 ttaaaaggaa aaaagtgtaa agcaactcac cccgcgttat ttgtacgctt tcagtagcac
     4141 cctacgcccc tctttttcaa aaaaagagag gggtcggggg tgagtcgttt ttagacttcg
     4201 gagcacttga attgatgact tttgctttta cccttttctc ttttcacttt ttaaaaaagg
     4261 cggcggttcg atgatcgatg tgcagaatat gcgggatgaa c
//
LOCUS       DASVMB010000081         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_452212,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000081 DASVMB010000000
VERSION     DASVMB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452212"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF531_02635"
     CDS             <1..306
                     /locus_tag="VF531_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7712894.1"
                     /translation="SQANGYFNKLAAPGTVLNESLRRTYDNPVNRGRLERIKALCGEL
                     SLSTTQIVLGYLLSQPFTTIPVVGCHTVEQLRDSMSAENVVFDESTMRYLRDGLPGV"
     gene            344..667
                     /locus_tag="VF531_02640"
     CDS             344..667
                     /locus_tag="VF531_02640"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX7712895.1"
                     /translation="MERYGMIIKVKPEKLEEYKRLHAAVWPEMLDLIRGCRIKNYSIF
                     YQGGYVFSYYEYYGDDYQKDMAKFDAAPINRKWGALCRPCLEPVPERAEGERWVKMEE
                     FFHLD"
     gene            857..2272
                     /gene="uxaC"
                     /locus_tag="VF531_02645"
     CDS             857..2272
                     /gene="uxaC"
                     /locus_tag="VF531_02645"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX7712896.1"
                     /translation="MGKTFITGDFMLHGKTAKVLYHDFAEAMPIFDYHCHLQPKDIAE
                     NRRFDNLTQIWLGGDHYKWRAMRTNGVDERFITGDADDEEKFKAWAETVPFTVRNPLY
                     HWTHLELRNPFGYTKLLEPQTAMEAYQHCNRLLQTEPFRVRGLMEHFNVKIVCTTDDP
                     TDSLEYHKALAADSNFGVKVLPAFRPDKAMAVESPELFNAWVGRLEKASAMSIHNYRE
                     FLEALRKRHQFFHEMGCRLSDHALEAPYAADYTETEIETIFQKVRGGATPDPAENVKF
                     KAAMMYEFGVMNAEKGWTMQLHMNALRNVNSRLLKLLGPDTGFDTIGDFLPAQPLARF
                     LDRLDKENHLPKTILYSLNPMDNEVLATVIGCFQDGSVPGKLQFGSAWWFNDQKDGMK
                     RQIEALSQMGLLSRFVGMLTDSRSFLSYPRHDYFRRILCDMMGQDVENGELPDDTRWL
                     GKIVQDICYNNAVRYFGIEGK"
     gene            complement(2377..2976)
                     /locus_tag="VF531_02650"
     CDS             complement(2377..2976)
                     /locus_tag="VF531_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7712897.1"
                     /translation="MGEQFINIYVRFFFLLTPFFLLSTFLAMTREMTPTVRKKLAVKV
                     TLAVIVICITIFLIGDYIFSLFGITLNSFRIGTGILLILSAVSLVLGSDKPQIREESE
                     DISVVPLAIPVTVGPATTGALLVMGVELQRFWERVVGIAALCAAIFSVGLLLYLSGSV
                     ERILKKQGLTILSKVTGLVLAALAAQMIMTGVLGFLGRS"
     gene            complement(3243..>3431)
                     /locus_tag="VF531_02655"
     CDS             complement(3243..>3431)
                     /locus_tag="VF531_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7712898.1"
                     /translation="FLACLLFGFAEALEGQFQILGLDVPKQFLHMMPYALTILLILGV
                     VGRSTAPAADGVVYEGRK"
BASE COUNT          865 a          805 c          920 g          841 t
ORIGIN      
        1 tctcaagcca acggttactt caacaagttg gccgcacccg ggaccgttct aaacgaaagt
       61 ctcagaagga cttatgataa tccggtgaac cggggccggt tggaacggat caaagcgtta
      121 tgcggagaac tatccctttc aaccactcaa atcgttttgg gatatctgtt gtcgcaaccg
      181 ttcaccacca ttcctgttgt gggatgtcat acggtcgagc agcttcgaga cagcatgagc
      241 gcggagaatg ttgtatttga tgagtcaacc atgaggtact tgcgtgacgg tttgcccggg
      301 gtttgagttt cgggaccatt gcggaagaaa tggaggattt ctggtggaaa ggtatgggat
      361 gatcatcaag gttaagccgg agaaacttga agaatacaaa cggcttcatg cggcggtttg
      421 gccggagatg ttggatctga ttcgtggatg ccggattaag aattattcca tcttttatca
      481 gggcggttat gttttcagtt attatgaata ctacggtgac gattatcaga aggacatggc
      541 taagttcgat gcggctccga tcaatcggaa atggggggct ctttgccgac catgtctgga
      601 gcctgttcct gaacgggcgg aaggggaacg ctgggtcaag atggaagagt tcttccactt
      661 ggattagacc ggattcaatt tttttgcctg aaaaatccaa tttgatgcag tgcctttgtc
      721 tttccggtgt ggtacaataa tgaagggatc gttctcttgg atatcccaaa gcagtccctc
      781 ggtacctcgt cgttttttat ttaggtcagt gggttatggc aaaatgtaaa cgcttacttt
      841 ttaagggggg gtttttatgg gcaagacttt tataaccggg gattttatgc tccacggcaa
      901 gaccgccaag gttctctacc atgattttgc cgaagcgatg ccgatctttg actaccattg
      961 tcacctgcaa cccaaggata ttgcggaaaa ccggcgtttt gacaacctga ctcagatctg
     1021 gctcggcggc gatcactaca agtggcgggc catgcggacc aatggggttg acgaacggtt
     1081 cattaccggc gatgccgatg acgaggagaa gtttaaggcc tgggctgaaa ctgtaccatt
     1141 tacggtgcgg aacccgttgt atcactggac ccatctggag ctcaggaatc ctttcggata
     1201 taccaaactc ttagagccgc aaaccgccat ggaagcttac cagcactgca accggctgct
     1261 acagaccgag ccgttccggg tccggggact catggaacac tttaatgtca agatcgtctg
     1321 cactaccgat gatcccacgg attcactgga gtaccacaag gcgctcgccg ctgattccaa
     1381 cttcggggtc aaggtactcc cggctttccg gcctgacaag gcgatggctg tagagtctcc
     1441 ggaactcttc aacgcctggg tgggccggtt ggagaaggcc tcggcaatga gcattcataa
     1501 ctaccgggag tttttggagg ctttaaggaa acggcaccag ttcttccatg agatgggttg
     1561 ccgactctcc gatcatgcct tggaggcccc ctatgccgcc gattataccg agacagagat
     1621 cgaaaccatc tttcaaaagg ttcgcggtgg tgcgacgcct gatcccgcgg agaacgtcaa
     1681 attcaaagcg gcgatgatgt atgagttcgg agtgatgaat gccgaaaagg gatggacgat
     1741 gcagctgcat atgaatgcgc tccgcaacgt caattcacgt ttactgaaat tgcttggccc
     1801 ggataccggg ttcgatacca ttggcgactt tttacccgcc caaccactgg cccgttttct
     1861 ggaccgcttg gacaaggaga accatctgcc caaaacgatt ctttatagct taaatcccat
     1921 ggacaacgag gtgctcgcca cagtcatcgg ctgtttccag gacggttcgg tcccggggaa
     1981 actccaattc ggcagtgcct ggtggtttaa tgatcaaaag gacggaatga aacgccagat
     2041 tgaggcctta tcgcaaatgg gcttgttaag ccggtttgtg ggcatgctga ccgattcgcg
     2101 cagtttcttg tcttatccgc ggcacgatta tttccggaga attctctgcg atatgatggg
     2161 gcaggatgtg gaaaacggtg aattgccgga tgataccagg tggctgggta aaatagtcca
     2221 ggatatctgt tataacaatg cagtgaggta tttcgggatc gaggggaagt aaatcaatcc
     2281 ctcgtaaagg ttacgataac ggcggtgctg aaattgcacc gccgttttga ctgtccttgt
     2341 tagagacgat gcatttccgc ttgagatgtt tttgttttag cttcgtccca aaaaacccaa
     2401 aaccccggtc atgatcatct gcgcggcgag ggccgccagt accaggccgg tcaccttgct
     2461 caggatggtc aatccctgtt ttttgaggat gcgttctacc gaaccgctga ggtataggag
     2521 caggccgaca ctgaagattg ccgcgcaaag ggcggcaatt cccaccaccc gttcccaaaa
     2581 ccgctgtaac tctacgccca tcaccaggag ggctccggta gtcgccggac ccactgtgac
     2641 cggtattgcc aggggtacca ccgaaatatc ttctgactcc tcgcggattt gaggtttgtc
     2701 cgatcccagc accagtgaaa ccgcagagag tatcaggaga ataccggtac cgatccggaa
     2761 ggagtttaag gtgattccaa agagcgaaaa gatgtaatcg ccaatgagaa agatagtgat
     2821 acagatgacg attaccgcta aagttacctt aactgctaac ttctttcgga ctgtcggcgt
     2881 catctcccgg gtcatcgcca gaaaagtgga caggagaaaa aacggggtca acagaaagaa
     2941 gaaccgcacg tagatattga tgaattgttc gcccatgggc acgctcctta tagggaaatg
     3001 atcttccccc gtcatggaag aatggattca tctgatcata tcaggaaacg gacgaccatt
     3061 gcaaatggta atgagaaatt ttcaaaaaaa ttctcatttt ctacgagccc ttatgagtat
     3121 aggcaacagg gaacaggcaa cagtattata agtaatttat tttagtccaa ggtctttata
     3181 tttttgaccc caaataaaaa agaaagccgg ctcagatgag ccggccgttt gtatttttgc
     3241 gcttactttc ttccttcata gactacgccg tctgccgccg gagcggttga acggcctact
     3301 acgcccagga ttaacaggat cgtcagggca taaggcatca tatgcaggaa ttgtttgggc
     3361 acatcgagtc cgaggatctg gaactggcct tccagggctt cggcgaaacc aaagagcaaa
     3421 caggcgagga a
//
LOCUS       DASVMB010000082        10663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_157347,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000082 DASVMB010000000
VERSION     DASVMB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157347"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF531_02660"
     CDS             complement(<1..387)
                     /locus_tag="VF531_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712899.1"
                     /translation="MKRLAGSFAVGFLLFGLFFQVTIAQDNTDQANNQQLLAQLKKQT
                     ELTDLRTENSMTFQDKSGRKTVYLSDIPLNYQEKDGKYFPIETKLISDKVTKGLKFRH
                     RALKNVLQAQFADFSDGGLLLNHGKSR"
     gene            complement(421..2097)
                     /locus_tag="VF531_02665"
     CDS             complement(421..2097)
                     /locus_tag="VF531_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEX7712900.1"
                     /translation="MKKRILAFISLCLLTTNFMVVNSSSIGNMAINTVINGRIKGAME
                     LSPKMAHIQIRRVGESIENLPQTIEADSKGGFELTLKETGIYQLRFSAVDYQTVMVPV
                     WITDSDKRVSLDIVLSPQKYMTTLDEIKIIGDWNNFNPRQAETMQKEPDGTFVYEREV
                     TADTVKYQIMGATDNSLIPGTMADGYEYDNNGNYRAVIKVVPGRITIIFDPAQLHQDQ
                     PYEPNIVFDKDHQEFERLFEITKLYTQKYSEYLSAFTAYRENNGNVRKFSFDSSSLWN
                     DLDGILMDGKSSSMIRQYAAFTMIEMLRMNPTYENGLLDEFLNIIPPESPIWSMDPSA
                     VYIFSNCFEKGKAGTILWDFYKKNPDHRVQAQSLVGLVKNAKANGDKANYPLLYQELN
                     DKYRDIPEIQYDLKNLNPDKKIAQGKPVPDFQVLLLEPGKVISNLSLRGKFYLIDFWA
                     SWCGNCGDEMPFLHAAYQKYKDRNFEILSLSFDQKSEDITKFRLKWPMLWLHGWVANG
                     FESELAQRFEVAGIPKAILVGPDGNILATENLRGESLEKTLEMYLVSEQK"
     gene            2616..2987
                     /locus_tag="VF531_02670"
     CDS             2616..2987
                     /locus_tag="VF531_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712901.1"
                     /translation="MEDNKVAMLLEDLRAQFRVFGEGLQTLNEKMDQEFNEVKGDIRN
                     LQHEVANLKHDVSDLKNDVSGLKNDVSGLKNDVSGVKTDVAELKKEFSSSRAENRQEH
                     QQLQQAIAELDTEVVKLKRVK"
     gene            3246..4349
                     /locus_tag="VF531_02675"
     CDS             3246..4349
                     /locus_tag="VF531_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7712902.1"
                     /translation="MKLTAVIPVYRDPKRVSDIVRKLLASDYPNHEIVIVVDGETNSR
                     IEEALAPYREQIRIHYNQAQLGKSASLNRIAAELRTDLLLFLDNDILLPGDPGFVTRV
                     VSVMQDCDISELPKEAVAVSLISKMMRYEFLGFAMTTYVLSKLSGRCPAMNGAAFAVR
                     KELFDKLGGFKNVVNEDMDFAARSFRQGARFSYNPRLKVENEVPTTLAEWVKQRKRWA
                     LNNVLWLRNNFRLLAAHFFKSFSFFISGILLLLPFFTYMVVFALMKRVHLTPFLPVLF
                     MTAQHIHMSAGLLLWLTQLNLIFGEGLVPTLMGLSVSSLIYFGFAKFLRFRFNFLEYL
                     LFYFIYSPIWLLANIIVWVGMALKIDIQVDWKV"
     gene            complement(4390..5508)
                     /locus_tag="VF531_02680"
     CDS             complement(4390..5508)
                     /locus_tag="VF531_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7712903.1"
                     /translation="MTNRERTLAVLNYQSYDRLPIVHFGFWQETLDKWVQEGHLKPEE
                     ILNISDGSPAERVIAGKLGFDFNMMTNYNPATALLPKFEEQVLEELPDGMKKIMNGDG
                     VIVLQKRGATSIPAEVDHLLKGRKEWQEHYLPRLQFSESRLDPEALAGLADTTAREYP
                     IGIFCGSLFGEIRNWLGLVGVSYLYVDDEDLYTEIIDTVADLCYKTTEWALKSGAKFD
                     FGHFWEDICFKNGPLVNPAVFAAKVGPHYKRITDLLHRYGVSIVTLDCDGVIDTLIPT
                     WLENGVNTMFPIEVGTWNASITPWREMYGRELRGVGGMNKTVFARDRAAIDAEIERLK
                     PLVDLGGYIPCPDHRIAPDAKWENVQYYCERMRRTFGG"
     gene            5636..6490
                     /locus_tag="VF531_02685"
     CDS             5636..6490
                     /locus_tag="VF531_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7712904.1"
                     /translation="MKARFELDIAPSSISMNHTPSLAARDLPCYLQSWGRFQARAGYF
                     TERSEYDSFLFLYTIGGSGLITYRGREYSLSPEQAAFIDCSEYHFYRTGPEGFWELEW
                     LHINGTALRHYFELLNGDDLQLMAMAAAPEVPQYLTTIHRLLTEDDPTKDYRFVPLVL
                     EILTQLVTAKLHPTDSRYRMMIEQILAYIATNYSAKLYCDDFLELTHLSKFHLLRLFK
                     KHTGVSLYEYLTNYRINVSKSLLRETQLTVTEIAARVGYGSPNNYIREFKKLAGTTPL
                     QYRNRQSE"
     gene            complement(6613..7692)
                     /gene="uxuA"
                     /locus_tag="VF531_02690"
     CDS             complement(6613..7692)
                     /gene="uxuA"
                     /locus_tag="VF531_02690"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEX7712905.1"
                     /translation="MKMTFRWFGEGYDHITLDQIRQIPGRPGIVGALYDVPVGEVWPV
                     EKIKNLKDTVVQAGLELEVIESVNIHEDIKLGLPTRDRYIENYRTTLRNLSKFGIKVV
                     CYNFMPVFDWTRSDLAKVLPDGSNALSYEEAKVQQIDPNKLVEEMEANSNGFNLPGWE
                     PERLKTLKHLFEQYRGVDEPKLLSNLGYFLKAIIPVCEEVDIKMAIHPDDPPWSIFGL
                     PRIVKSKESLEKLLNLVDSTYNGLTLCSGCLGANPENDIPALIRYFGSRGRIHFGHVR
                     NIKIHSPRVFNETSHLSADGSLDLFEIMKAYHDIGFQGYIRPDHGRMIWGEKGRPGYG
                     LYDRALGIAYLNGLWEAIDKMKKSK"
     gene            complement(7767..9377)
                     /locus_tag="VF531_02695"
     CDS             complement(7767..9377)
                     /locus_tag="VF531_02695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX7712906.1"
                     /translation="MLKLSRENIKERREWEKAGIELPEFDYGKMVLATGQNPGWVHFG
                     AGNIFRGFTAALQQRLLNLGKADTGIIAAETYDFEIIDKIYKPYDNLSLLIIMKPDGT
                     LDKKVVASVSESLVGDVSRKADWQRLQEIFTKSSLQMASFTITEKGYNLKKISGEYFD
                     EVVKDLQEGPLNPSHIMSKVTALAYTRYKNGELPIAFVSMDNCSHNGERLHASVETIA
                     KQWVENGKVESGFLTYINDHNKVTFPWSMIDKITPRPSEKVQTALNSIGFGNTEILCT
                     AKNTFIAPFINAEGPQYLVIEDSFPNGRMPLEAAGVIFTDRQTVDRVEKMKVCTCLNP
                     LHTALAVYGCLLGYNLIADEMKDAELKKLVERIGYQEGLPVVVNPGVIQPQDFIREVI
                     EQRLPNPNIPDTPQRIASDTSQKVGIRFGETIKAYNSKGKTAELKYIPLAIAGWCRYL
                     LGLDDQGKPMPLSPDPMLEPLKSYLTGVSLGDENSGQDSLKPILSNERIFGVDLYQAG
                     LGDKIEGYFKEMISGNGAVRTTLRKYIN"
     gene            complement(9550..10620)
                     /gene="wecB"
                     /locus_tag="VF531_02700"
     CDS             complement(9550..10620)
                     /gene="wecB"
                     /locus_tag="VF531_02700"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7712907.1"
                     /translation="MRILTIVGARPQFVKAAVVSRALSGRAEEILVHTGQHYDFNMSE
                     LFFRELALAAPAYNLGIGSGSHGEQTGRMLGAIEEVLGKETPDLVLVYGDTNSTLAGA
                     LAAAKIHIPVAHVEAGLRSFNRRMPEEINRVLTDHLATWLFAPTATAVANLSREGLTS
                     GVDLAGDVMYDAFLLLSQQAGRYRSILETLELKPRRFALLTLHRAENTDDPARLAAIL
                     ETLDTLDLPVIFPAHPRTRAKIMELGLQLAGKALRPIDPVGYFEMLNLESNAAVILTD
                     SGGVQKEAYMAKTPCITLRDETEWVETVTACWNRLAGADPEKIRAALRLDWVGSDYPL
                     LFGDGAAADRIATVVTRADNRF"
BASE COUNT         2612 a         2773 c         2349 g         2929 t
ORIGIN      
        1 acggctttta ccgtgattca acagcagccc gccatcggag aagtctgcga attgggcctg
       61 taagacattt tttaatgccc gatgtcggaa ttttaagccc ttggtaactt tatccgatat
      121 taatttggtt tcaatcggaa aatacttgcc atccttttct tgatagttca atggaatatc
      181 cgataaataa acggtttttc gcccgctttt atcttggaag gtcatactgt tttcagttcg
      241 cagatcggtt aattcggtct gctttttaag ttgtgccaat aattgctggt tgtttgcctg
      301 gtcggtatta tcttgagcaa tcgtaacttg aaaaaacaat ccaaacaaaa gaaaaccaac
      361 tgcgaaagaa cctgctaacc ttttcattct atttccccct taaacgggta attgattcac
      421 ctatttctgt tcggagacca agtacatttc aagcgtcttc tccaagctct caccgcgtaa
      481 attttcagta gccagtatgt tcccgtccgg cccgaccaga atcgctttcg gaattccagc
      541 cacctcgaaa cgttgtgcta attcgctttc aaaaccgtta gccacccagc cgtgtaacca
      601 gagcattggc cattttaaac gaaatttagt aatatcttct gatttttgat caaacgatag
      661 actaagaatc tcaaaatttc tatctttata cttttggtaa gcagcatgta gaaaaggcat
      721 ctcatcccca caattgccac accagctagc ccaaaaatcg atcaaataaa acttaccgcg
      781 taggcttagg ttagaaataa ccttcccggg ctcaagcaac agcacttgaa aatcgggcac
      841 cggtttacct tgagcgattt ttttatccgg attcaagttt ttcaagtcat attgaatctc
      901 cggaatatct ctgtatttat catttaattc ctgatacaaa agaggatagt ttgctttatc
      961 cccgttggct ttcgcatttt taaccaaacc gacaagtgat tgggcttgga cacggtggtc
     1021 cggattcttt ttataaaagt cccataaaat cgtcccggct tttccttttt caaagcaatt
     1081 gctgaaaata tacactgcag atggatccat gctccatata ggcgactcgg gaggaatgat
     1141 gtttaggaac tcatccaata aaccattctc ataggtggga ttcatcctta acatctcaat
     1201 catcgtaaaa gccgcatatt gccgaatcat cgaagaagat ttcccatcca ttaaaatacc
     1261 atctaaatca ttccatagac tggaactgtc aaaggaaaac tttcttacat ttccgttatt
     1321 ttcacgatac gctgtaaacg ccgacagata ttctgaatac ttctgtgtat atagtttagt
     1381 tatctcaaat agtctttcaa attcttgatg atctttatca aaaacaatgt ttggttcata
     1441 cggctggtct tgatgcaatt gtgccggatc aaagataatc gttattcttc ccggaactac
     1501 cttaatcacc gcgcgatagt tgccgttgtt gtcgtattca tacccatccg ccattgtgcc
     1561 cggaattaaa gaattatctg ttgcgcccat aatctgatat ttgacggtat cggctgtaac
     1621 ttccctttca taaacaaagg ttccatccgg ttctttctgc atggtttccg cttggcgggg
     1681 attaaaatta ttccagtcgc cgatgatttt tatctcatcc aaagttgtca tatatttttg
     1741 tgggcttaag acaatatcta ggctgactct tttatcggaa tcggttatcc aaacaggcac
     1801 catcacagtc tggtagtcta ctgcactgaa ccttagttga tatatccctg tctcctttag
     1861 cgtgagttcg aaaccgcctt ttgaatcagc ctcaattgtt tgaggaagat tttcaataga
     1921 ttcaccaact cttcgtatct ggatatgcgc cattttaggg gaaagttcca tagctccttt
     1981 aattcgtcca ttgataactg tgtttatagc catatttcct atcgaagaac tgttaacaac
     2041 cataaagttc gtagtcaata aacaaagtga aatgaatgcc aaaatccgtt ttttcatcgt
     2101 tcatccctct caatctgttt tgtcgaataa tgccgttttt taacggatat caaaatataa
     2161 cccggccttt cctgcccggc cgggtttttg gtttcgcacg gattcaattg catttacgga
     2221 gtttctcaaa ttccaaaacc ccctagagaa tttattgtat tcttctccat cctccattat
     2281 tttcctgcag aaaaattcat tccttttata gtatttagta tatcggcact ttatcaacaa
     2341 aataaatttg tccagattca cgaataccac tcattccgca ttccacccct ctttccggcc
     2401 tgcccggaga gaggaaacag aaatcaaccc acctacctct agctcctccc tgacagggaa
     2461 gagcaagaga taggtgggca agcgacagga ggaggtccat ctatacttcc cgtaactaac
     2521 ggggcgagtc gttcactcgc ttccctggaa acttgtccca aaatgaaata gtttatgata
     2581 taattatttt gaagttgctt tgcggggtga tttggatgga agataacaag gtagcaatgt
     2641 tgcttgagga cttacgagcg cagtttaggg ttttcggaga aggattgcaa actcttaatg
     2701 agaagatgga tcaggagttt aatgaggtta aaggtgatat tcgaaatctg cagcatgagg
     2761 ttgcaaattt aaaacatgat gtatctgatt taaagaatga tgtgtctggt ttaaagaacg
     2821 atgtgtctgg tttaaagaac gatgtgtctg gcgtaaaaac tgatgtagcc gaattaaaaa
     2881 aagaattcag cagttctaga gcggagaacc gtcaggaaca tcagcaattg cagcaagcca
     2941 ttgccgaact tgatactgag gttgttaaac tgaaacgggt gaaataggtg gggccctcac
     3001 agccttgaga taatcgccag ttctgagctt ccccctctct gccgtccggc ggcaaagagg
     3061 gggaaaattg ttttatcgcg gaatttcctg aaatatgatc ccggcggccg gaaccgattt
     3121 catccggaag aagattattc ctttttaagt aacggttttc gggaatcttt cgcaactcat
     3181 cgcgttataa ctagacgaat tgttgcgccg gttttatttt gcgattccat ttccgaggtg
     3241 atccgatgaa gttgaccgcc gtgatcccgg tatatcgtga tcccaagcgc gttagtgata
     3301 tcgtccgaaa actgctcgcc agcgactacc cgaatcatga gatcgtaatc gtcgtggacg
     3361 gagagaccaa ttctaggatt gaagaggcgc tggcccctta cagggaacag atccggattc
     3421 attacaacca ggctcagctc ggcaagtcgg cttcactcaa ccggatcgcc gccgaactcc
     3481 ggactgattt gctcctgttt ttggataacg atattctgct tcccggggat cccgggtttg
     3541 tgacgcgcgt cgtcagcgtc atgcaagatt gcgatatctc cgaattaccc aaggaagcgg
     3601 tggccgtctc gctcatctcc aagatgatgc gctatgagtt cctgggattt gccatgacca
     3661 cctacgttct ttcaaagctc tccggacgtt gcccggcgat gaatggcgcc gcctttgcgg
     3721 tccgcaagga gctctttgat aaattgggcg gtttcaagaa tgtggttaat gaagacatgg
     3781 actttgcggc ccggtccttc cggcagggcg cccggttcag ctacaatccg cggctcaagg
     3841 tggagaacga agtcccgacg accctcgccg agtgggtaaa acaacggaaa cgctgggcgc
     3901 taaacaacgt cctttggtta aggaataact tccgacttct ggcggcccat ttctttaagt
     3961 cgttctcttt tttcatatcg ggtattttgc tattattgcc gttcttcacc tatatggtag
     4021 tttttgcctt aatgaaacgg gttcacctca ctcctttcct gccggtactc ttcatgaccg
     4081 cccagcacat ccatatgagc gccggactgc ttctctggct gacacagctc aacctgatct
     4141 tcggggaagg tttggttccg acattgatgg ggctttcggt ctccagcctt atctacttcg
     4201 gtttcgccaa attcctgagg ttccgtttca atttccttga atacctgttg ttttacttta
     4261 tctactcacc tatctggctt ttggccaata tcatcgtatg ggtcggaatg gcgctcaaga
     4321 tcgatattca agtcgattgg aaggtgtaaa taaatatccc ccggcggagc cgggggacat
     4381 ctactcaaat tatcctccaa aggttcgccg catccgctcg caataatact gcacattctc
     4441 ccatttggcg tcgggggcga tccggtgatc gggacaggga atatatccgc ccagatccac
     4501 caggggttta agccgttcga tctctgcgtc gatcgccgcg cgatcccggg caaaaacagt
     4561 cttattcatt ccgccgaccc cccggagttc ccgcccgtac atctcgcgcc aaggagtgat
     4621 actcgcgttc caggtcccaa cctcaatcgg gaacatcgta ttcacaccat tctccagcca
     4681 ggtgggaatg agggtgtcaa tcacaccgtc gcaatccagg gtgacaatgg aaacgccgta
     4741 gcggtgtaac agatcggtaa tccgtttata atgaggcccg actttggccg caaagaccgc
     4801 cgggtttacc agcggcccgt ttttgaaaca tatatcctcc cagaagtggc cgaaatcaaa
     4861 cttggctccg ctttttaagg cccattccgt ggttttataa cagagatcgg caacggtatc
     4921 aatgatctca gtgtatagat cctcgtcatc cacatacagg taactaacac ccaccaatcc
     4981 aagccagtta cgaatctctc cgaatagact cccgcagaaa atgcctattg ggtattcacg
     5041 ggctgtggta tcggcgagtc cggccaaagc ctcgggatcg agccgggatt cggagaactg
     5101 aaggcgaggc agataatgct cctgccactc cttcctccct ttaagcagat gatcgacttc
     5161 cgcaggaatg gatgtggcgc cccgcttttg gagtacgatc accccgtcgc cgttcattat
     5221 tttcttcatt ccgtccggca actcctctaa cacctgctcc tcaaacttcg gaaggagcgc
     5281 ggtcgccgga ttatagttgg tcatcatgtt aaaatcaaat cctaatttac cggcgatcac
     5341 ccgttccgcc ggactcccgt cgctgatatt taagatctct tccggtttca gatgcccctc
     5401 ttgcacccac ttgtccagcg tctcctgcca aaatccgaaa tgaacgatag gcagccgatc
     5461 atacgattga tagtttaaga cagccaaagt ccgttcccga ttggtcatcc cgctccaccc
     5521 cacaggttta acatattttg ctcatatcat gttataatta tagtacagtt ttaaacggca
     5581 ttcaccgata gatcttgctg aaaaatatat aaatcttgct ctgggggagt ttgccttgaa
     5641 agcccgtttt gagttggata tcgctccaag ctccatttcc atgaaccata ctccgagcct
     5701 tgccgcccgg gatctcccct gttacctgca aagctggggc cggtttcaag cccgggctgg
     5761 ctatttcacc gaacggtccg aatacgacag tttcctgttc ctttatacca ttggtggttc
     5821 cggcctgata acctaccgtg gccgggaata ctccctaagt ccggaacaag cggcattcat
     5881 cgactgttcc gagtatcact tctaccggac gggacccgag gggttctggg agcttgagtg
     5941 gctgcacatc aacggcacgg cactacggca ctattttgaa ttgctaaacg gcgacgatct
     6001 gcaactgatg gccatggcgg cggctccgga ggtcccccaa taccttacga ccattcaccg
     6061 cttgcttacg gaggatgacc ccaccaagga ttaccggttc gtccctctgg tgctggagat
     6121 ccttacccaa ttagtcaccg ccaagctcca tcccactgat agccgttacc ggatgatgat
     6181 cgaacagata ctcgcctata tcgcgaccaa ttactcggcc aaattgtact gtgacgactt
     6241 tctggaattg acccacttaa gcaaattcca tctgttgcga ctattcaaga aacataccgg
     6301 cgtcagcctc tacgagtatc tgaccaatta ccggattaat gtttccaagt cattgttgcg
     6361 cgagactcag cttacggtca ccgagatcgc cgccagagtc ggatatggca gccccaacaa
     6421 ctatatccgc gaattcaaga aactggcggg aaccaccccg ctccaataca ggaaccggca
     6481 gagtgagtga acccctgggg tagccggggg ttttcgtaca tacaaaaaac ctcacgttgg
     6541 acgtggggtt ttttatacag tcatgctccc ggtattgccg ggagcatgaa aaaaagccaa
     6601 tctctagcct catcactttg attttttcat tttatcgatg gcttcccaga gcccgttcag
     6661 gtaagcaatg cccagagcgc ggtcatataa accatacccc ggtctgccct tttcgcccca
     6721 gatcatccga ccatggtccg gccggatata accctgaaag ccgatatcat ggtaagcttt
     6781 catgatctcg aagagatcca gagatccgtc ggcggacaaa tgggaggttt cattgaagac
     6841 acggggcgaa tgaatcttga tattgcgaac atgcccgaaa tgaattcttc ccctggaacc
     6901 gaaatagcgg atcaaggccg gaatatcatt ctccggattg gctcccagac agccactgca
     6961 tagcgttaag ccgttataag tgctgtccac caggttgagg agcttctcca ggctctcctt
     7021 ggatttaacg attctgggca ggccgaaaat tgaccatggc ggatcatccg gatggatggc
     7081 catcttgata tcgacctctt cgcaaaccgg aatgattgct tttaggaaat accccagatt
     7141 cgacaggagc ttgggttcat ccactccccg gtattgctcg aatagatgct tcaaggtctt
     7201 caacctttcg ggctcccagc ccgggaggtt aaatccgttg gagttggcct ccatctcttc
     7261 gaccaactta ttggggtcaa tctgttgtac ctttgcttcc tcgtatgaca gcgcgttgga
     7321 accatcggga agaaccttgg ccagatccga acgggtccag tcgaatacgg gcataaagtt
     7381 gtagcatacc actttgatcc cgaatttcga caggtttctc aaggttgtcc gatagttttc
     7441 gatgtaccgg tctctagtcg ggagtcccag tttgatatcc tcatggatat tgacgctctc
     7501 gatcacctcg agttccaacc cggcctgtac taccgtgtcc tttagattct taatcttttc
     7561 aaccggccat acctcgccca ccggtacatc ataaagggcg cccacaatac cgggccttcc
     7621 cggtatctgc ctgatctggt ccagagtgat atgatcgtaa ccctctccga accacctgaa
     7681 tgtcattttc atccttgtta tcctcgtagt tgatatttga ggtgctgctt tgacaacccg
     7741 tttttgaagt taacatcccc ttattcttaa ttgatgtatt tccgtaatgt tgtccgaacg
     7801 gcgccattcc cggagatcat ttccttgaaa taaccctcga tcttgtctcc caatcccgcc
     7861 tgatacaggt ctactccaaa aattctttca ttggagagaa tcggttttaa actgtcttgc
     7921 cccgaattct catcgcctaa acttacgccg gtaaggtacg actttaaagg ttccagcatc
     7981 gggtccggac ttaagggcat gggctttccc tgatcgtcca agcccagcag ataccggcac
     8041 caaccggcaa tcgccagggg aatatatttg agttccgcgg ttttcccttt actgttgtac
     8101 gctttgatgg tctccccaaa ccggatacct accttttgtg aggtatcgga ggcaattctt
     8161 tgcggcgtat ccggaatgtt gggatttggc aacctttgct caataacctc gcggatgaaa
     8221 tcttggggtt gaatgactcc cggattgacc accaccggta ggccctcttg ataaccaatc
     8281 ctttcaacca gctttttcag ttccgcatcc ttcatctcgt ctgcgatgag attataaccc
     8341 aacaggcatc cgtataccgc tagcgccgta tggagcggat tcaaacaagt gcaaaccttc
     8401 atcttctcta cccggtccac tgtctgtctg tcggtgaaga tgactcccgc ggcctcaagc
     8461 ggcatacggc cattggggaa actgtcctca atcaccagat actgagggcc ttcggcatta
     8521 ataaacggcg caatgaaggt gttcttggcg gtacatagaa tttcggtatt tccgaaaccg
     8581 atggagttaa gggcggtttg caccttctcc gaaggcctgg gggtaatctt gtcaatcatc
     8641 gaccagggga aggtgacttt attatgatca ttgatatagg ttagaaaacc gctttcaacc
     8701 ttgccatttt ccacccattg cttggcaatt gtctccactg aggcgtgaag cctttccccg
     8761 ttatgggaac agttgtccat acttacaaaa gcgatgggga gctccccgtt tttataacgt
     8821 gtgtaagcca gcgccgtaac ctttgacatg atatggctag gattcagggg gccttcttgt
     8881 agatccttca cgacctcatc aaagtattcc ccggatatct tctttaggtt gtatcccttc
     8941 tccgtgatag taaagctagc catctgcaga gaggatttcg taaagatctc ctgtaaccgt
     9001 tgccagtcgg ctttccggga aacatctcca accaggctct cactgacact ggcaactact
     9061 tttttatcca acgttccgtc gggtttcatg ataatcaata gacttaaatt gtcataaggt
     9121 ttataaatct tatcgatgat ctcaaagtcg taggtttccg cggcaataat tcccgtatcc
     9181 gccttgccta aattgagcag tctttgctgt agcgccgcag tgaagcctct gaagatattt
     9241 cctgccccaa aatgtaccca cccgggattt tggccggtgg cgaggaccat tttaccgtaa
     9301 tcgaattccg gtaattcaat tccggccttt tcccattccc gtctttcttt aatattttct
     9361 ctagacagct tcagcatgtg aagattcctt ccttcttcct ttccggattc aagggactct
     9421 cttaaatgta agcgcttact tttttaattc ttcatcgata atccataatc cttccctttc
     9481 ataaaaatta atctataaaa aagagctctc tatcccacga taaccccgaa atttagcaat
     9541 aaaaatctcc tagaacctat tatcagccct cgttacaacc gttgcgatcc ggtctgcagc
     9601 tgcgccgtcg ccgaaaagga gcggatagtc cgaccccacc cagtccaaac gcaatgcggc
     9661 acgaatcttt tccggatccg ctcccgccag gcgattccaa caggctgtca ccgtctcgac
     9721 ccactccgtc tcgtccctta gggtgatgca aggggtcttg gccatataag cctctttctg
     9781 aactccaccc gagtcggtaa ggatcacagc ggcattcgat tccaaattaa gcatctcgaa
     9841 gtaacccacc ggatcgatgg gccgtagcgc cttcccggcc agttgcagcc ctagctccat
     9901 gatcttggcc cgggttcggg gatgagccgg aaagatcacc ggtagatcga gggtgtccaa
     9961 ggtctcaaga atcgcagcca gccgcgccgg atcatcggtg ttctccgccc gatgcagggt
    10021 gaggagcgcg aatcgcctcg gtttgagttc aagcgtctcc aggatggacc tatacctccc
    10081 cgcctgttgc gacaggagta aaaaagcgtc atacatcaca tcccctgcta agtccactcc
    10141 ggaggtgagc ccttcccggc tcaaattggc gactgcggtt gcagtcggcg caaatagcca
    10201 agtggcgaga tgatcggtga ggacccggtt gatctcttcc ggcattcgcc ggttaaaact
    10261 acgtagtccc gcctcgacat gagccacagg gatatgtatt ttcgcagcgg ccaacgcccc
    10321 ggcaagagta gaattggtat ctccatatac caacaccaga tcgggagttt ctttaccaag
    10381 aacctcttca atggccccaa gcattcttcc ggtttgctcc ccgtggcttc ccgaaccaat
    10441 ccccaggtta taggctggcg cagccaaagc caattccctg aagaagagtt ccgacatgtt
    10501 gaagtcgtaa tgctgtccgg tatgaaccag gatctcctca gcccgtccgg atagagccct
    10561 ggaaacgacg gctgccttga caaattgggg ccgggcccca acaatcgtta gaatcctcat
    10621 ctggaccccc gtttattggt atagaactcc tgaattttag cga
//
LOCUS       DASVMB010000083        13532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_232763,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000083 DASVMB010000000
VERSION     DASVMB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232763"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VF531_02705"
     CDS             <1..539
                     /locus_tag="VF531_02705"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7712908.1"
                     /translation="NLFFPPLPLETATIFAGYLSGTGRGSLIAIIAATTLGMFTGSII
                     LYILAEKYGNRIIFQTPLRKVITRKSYEKAACWFEKYGLAAVFLGKLVPGMSLYTVIS
                     CGVLGWPVSRAAPAFGVSNLLFFGLLALLGRTAGRHWSRILETLRHINQATLLLAGVA
                     LVVLAVIKIRSRRRERNR"
     gene            complement(553..2208)
                     /gene="hcp"
                     /locus_tag="VF531_02710"
                     /gene_synonym="priS"
     CDS             complement(553..2208)
                     /gene="hcp"
                     /locus_tag="VF531_02710"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HEX7712909.1"
                     /translation="MFCFQCEQTAGGTGCTSAGVCGKDHTTAALQDLLLHASAGIAMY
                     MHRARARDRKIDHFLLEALFTTVTNVNFDPDRLRSLLERAGALKEVARKLYETESRQN
                     GGIPERLGGPAEWVPATNLEGLVKQAEQVRPELEREQLGPDRFGLKNLVSFGLKGMAA
                     YAEHARILGVEDDAVYDFCYEILDFLTRDDFTETELLDRAMQVGKVNLRVMEMLDQAN
                     TETYGHPVPTPVRITPVQGKAILISGHDLKDLEELLKQTAGKSVNIYTHGEMIPAHAY
                     PKLKQYPHLVGNYGGAWHDQREEFDKFPGAILMTTNCIQRPKESYKGRIFTSGLVAWP
                     GVTHISGRNFAPVIQAALATEGFRETEPERTIMVGFARNAVLGVADKIVAAVQKGAIR
                     HFFLVGGCDGAKPGRDYYTQFVRQIPQDCVILTLACGKYRFNRMELGSIEGIPRLLDI
                     GQCNDAYSAIKIAQALAEVFHTDINSLPLSLVLSWYEQKAVCILLTLLYLGIQNIRIG
                     PSLPAFVTPAAFQALQKQFKLKAITTPEEDLRAILHTPEPARA"
     gene            2621..3457
                     /locus_tag="VF531_02715"
     CDS             2621..3457
                     /locus_tag="VF531_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7712910.1"
                     /translation="MVHIFTDTTSTLTLEEFKKYDIHPIPIIINQGEESRRELFDITP
                     EEFFKNQRAGVKYTTSQPSPAAYLDAFKPIIEAGDEIVCIIFSGGISGTFDNIYFTAQ
                     TYNADRITVFNSGLTAFCLAYMAIKAREMAGRGASRAEIIAYLEALHSRCRIYFLVES
                     LHYLHEGGRLSGAQALIGSIIQIKPIIWFEPDGKMSAFEKIRTTKAAKNRLIELTKER
                     ADIGIEAIALHYGDNTAEATEYAREIETAVGIKPHLIPISPVLCAHTGPDILGICTVT
                     KE"
     gene            3482..4318
                     /locus_tag="VF531_02720"
     CDS             3482..4318
                     /locus_tag="VF531_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7712911.1"
                     /translation="MIKIVTDTTCAMTLDEYGKYGIIAVPLYVRQGEDTQRELFDISY
                     DQFYKSQRTGTKFTTSQPDPNSFLEVFRPIVEAGDEIICVTLSGKISGTINSVTLAAQ
                     MLNSDKVTVIDSKQSGFGQASMALRARELANSGASREDIVRDLDDLQSRTRTYFMVES
                     LRYLYEGGRLSGAQALIGSLIQIKPIIWFDAEGTMEAIEKIRTLKAAKTRTMELIQER
                     VARGVEKIALHYADNYDEAVEFGKMMEEIAGMPVPLIKLSPVLGTHTGPDLLGPCIIS
                     KN"
     gene            complement(4664..5077)
                     /locus_tag="VF531_02725"
     CDS             complement(4664..5077)
                     /locus_tag="VF531_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015955206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX7712912.1"
                     /translation="MKAKQIVVGQNVKPIKQEWAKQLRRKMTPAEKRLWFYLRDKKLH
                     GLHFRRQQVIEGFIVDFYCHAAGVVVEADGGIHELYKEYDAERDRIIASRGLQVIRFR
                     NDEIENDIYHVIKRILQVCESSKRSQLDLLRRDGV"
     gene            complement(5636..5833)
                     /locus_tag="VF531_02730"
     CDS             complement(5636..5833)
                     /locus_tag="VF531_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX7712913.1"
                     /translation="MKVTIDRFEGDYALVELSDRKMGRLSKTLLPSDIKEGDVVEISV
                     AARATATEEKKIRKLMGELFE"
     gene            complement(5837..6688)
                     /locus_tag="VF531_02735"
     CDS             complement(5837..6688)
                     /locus_tag="VF531_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7712914.1"
                     /translation="MHSFHRRLVLGLCQVLLVAAVVFTPVHAALQPLKVYFFDVGQAD
                     CILIRTPNSRNILIDAGTNESAERVLSYLTAQKISRLDLVIATHPHEDHIGGMDAVIQ
                     KYSINQISMPKAATNTRTFESLLLAIKAKGLKITTARAGLSFDLDPALKLELVAPNRE
                     SYENLNDYSAVLRIVYGRTAFLFAGDAGFTSEREMLRAGYDLRSDVLKVGHHGSNSST
                     ATGFLRVVGPSYAVISVGAGNDYGHPGKSTLKRLNKAGIKVLRTDQAGTIIFTSDGNT
                     VHYKAGE"
     gene            complement(7119..7418)
                     /locus_tag="VF531_02740"
     CDS             complement(7119..7418)
                     /locus_tag="VF531_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX7712915.1"
                     /translation="MDYRLRGFWIGAVILLAMGIIAVIRMGVYPEEVMAVAVVDRIEG
                     SYAVLEFSDRTVVNLPLRVCGTGIREGERLILRRDSGGTRRNMQRLVKLMRTLFE"
     gene            complement(7626..10187)
                     /locus_tag="VF531_02745"
     CDS             complement(7626..10187)
                     /locus_tag="VF531_02745"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX7712916.1"
                     /translation="MGNLMNFAGITGLTSREVKERQEREGFNELPTAGKRSILRIALE
                     VFKEPMFLLLVACGSLYLLLGDIGEALMLLGFVFVVMGITIFQERKTERALEALRDLS
                     SPRALVIRDNERQRIPGREVVRGDTIILAEGDRVPADAVLIDSLNLAVDESLLTGESV
                     AVRKNAVKEIQPMGAPGGDDLPFVFSGTLVVQGQGIAVVQGIGLSTEIGKIGKALQTI
                     GTEKTLLQQEIGRLVRNLAVLGLVLCLLVVIVFGLTRGNWLHGLLAGITLAMAMLPEE
                     FPVVLTVFLALGAWRMSQKKVLTRREAVVETLGSATVLCVDKTGTLTRNQMVLKRLYA
                     GGRFFTLNGAGTGPLPEGFHKLVEYAILASQKDPFDPMERALRQVGDSALSNTEHLHH
                     DWSLVQEYPLSKELLALSHVWKSPDGVDYVIASKGAPEAIADLCHLEPSVREDLNEKV
                     RRMADDGLRVLGVARASFQESQLPSGQHDFQFQFLGLVGLVDPVRATVPQAIQECYRA
                     GIRVAMITGDYPGTAQSIAKEIGLAKPEMVITGPEIEALSDEELQQRIAEVSIFARVV
                     PEQKLRIVNALKARGEVVAMTGDGVNDAPALKSAHIGIAMGERGTDVAREASALVLLD
                     DHFASIVTAVRQGRKIFDNLKKAMTYIFSVHVPIAGLSLIPVLFKWPLILLPVHIVFL
                     ELIIDPACSVVFEAEPEEADVMERPPRNPKEPLFSSRTLLVGLMQGFSVLVIVLGVFA
                     IAINNRELSEAEARSFTFATLIVANLGLILTNRSWSQTIFKSLRTPNTALWWVTGGAV
                     FFLGLVLNVPFLRSLFRFAPLHAGDLAICLTAGILSIAWFEGFKLLRRHRKFPTD"
     gene            10578..10751
                     /locus_tag="VF531_02750"
     CDS             10578..10751
                     /locus_tag="VF531_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712917.1"
                     /translation="MSVMMVQIIDRHSQMQLPPQDESNCSYPTIAYHCTREIQGPELI
                     KRWVDLIRNTRRH"
     gene            complement(10860..11591)
                     /locus_tag="VF531_02755"
     CDS             complement(10860..11591)
                     /locus_tag="VF531_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712918.1"
                     /translation="MKRNVLIVGWICFFLVAMPGTGMVWAADETGAVPAGTDADPEAT
                     ADVAAETKPAVMDYGSIENSVYRNDYLGMSLPIPGDWQVQGEAVVKQMLESGKEVLPD
                     EDKKVQAALDAAEQSSLVLLTVFRYPLGTQVEYNPSMVWVAEKVELYPGIQVGKDYLL
                     NVKRIMESGQVQFQFNKEIYPQVLGGVNFDTLETQVSIAQMTVYQRYYATVRKGYAIS
                     FVISYTTEDEAKMLENILTGLKFVQ"
     gene            11792..12418
                     /gene="udk"
                     /locus_tag="VF531_02760"
     CDS             11792..12418
                     /gene="udk"
                     /locus_tag="VF531_02760"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX7712919.1"
                     /translation="MLIIGIAGGTGSGKSTVAEALQRELGPENVALISQDSYYADLAD
                     LPFEERAKRNYDHPDAFENGLLIDHLRRLRNGETVEVPVYDFSQHARTSRKISVTPRP
                     VIVVEGILILIEAALRTEFDIKVYVDTDADTRVIRRITRDIEERGRTLESVCRQYLNT
                     VKPMHEAFIEPSKRYADIIIPVGGYNTVALSLLVSRIESYLQSISTND"
     gene            12695..13342
                     /locus_tag="VF531_02765"
     CDS             12695..13342
                     /locus_tag="VF531_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712920.1"
                     /translation="MKKQLFLLLSFLLITAIVLSGCGGGSPSVQVKTIEYQDDGNGFT
                     QFYTNDKRNYNYSFWHSDLTPEETTFPVEIQVKKLSGNKSMVHGAFFCYKDDHNFYRV
                     VIDTEGNYYIFKHNSNGTEPRTTIRGKTASPNLNTGYNTLNTIHVERDDEGQFTIKFN
                     NNYKNDTEVITFEDTTFTGGRCGFYVAVGDETQEQFPNTPVDVRFKQILPEGASI"
BASE COUNT         3366 a         3701 c         3133 g         3332 t
ORIGIN      
        1 ccaacctatt ctttccgcca ctgcccttag agaccgccac aatattcgcc ggttatctat
       61 cgggaaccgg cagagggtcg cttatcgcga tcattgccgc cacaaccctg gggatgttca
      121 ccggaagcat aatcctctat atcctggccg aaaaatacgg gaataggata atctttcaaa
      181 caccgctgcg caaggtgatt acccgaaaat cgtatgaaaa agcggcttgc tggtttgaga
      241 agtatggcct agctgctgtg tttctgggga aattagttcc ggggatgagt ctttatacgg
      301 tgatttcttg tggagtcctg ggttggcccg tttccagggc ggcgccagcc tttggcgtca
      361 gcaaccttct attttttggc ctgctcgcgt tattggggag aaccgccgga cgtcattgga
      421 gcagaatctt ggaaacattg cgccatatca accaggcaac tctgctgctt gcgggtgtgg
      481 ctctagtcgt attggcggta attaaaatcc gctcccggcg gcgggagcgg aatcggtgat
      541 ggatcttatt cattaggctc tggcgggttc gggggtatgg aggatcgccc ggaggtcctc
      601 ttccggtgtg gtgatggcct tcagtttaaa ctgcttctga agcgcctgaa aagcggcggg
      661 cgtgacgaaa gcaggcaaac tgggcccgat acggatgttt tgaatcccca ggtacagcag
      721 ggtcaagaga atacagactg ctttctgctc ataccaggag agaaccaggg agagcgggag
      781 gctattgata tcggtatgaa atacttccgc cagcgcctgg gcgatcttta tcgccgaata
      841 cgcgtcgttg cattgaccga tatccagtaa acgcgggatc ccctcgatgc tgcccaattc
      901 catccggttg aaccggtatt taccgcaagc cagcgtgaga atgacgcaat cctgcggtat
      961 ctgccggaca aattgcgtgt agtaatcccg ccccggtttg gcgccgtcgc atccccccac
     1021 caggaaaaag tgacggattg cccctttttg aactgcggcc acaatcttgt cggctacccc
     1081 cagtaccgcg ttacgggcga agcctaccat gatcgtccgt tccggttcgg tctcccggaa
     1141 accctccgtc gccaatgccg cttgaatcac gggagcgaaa tttcggccgg agatatgagt
     1201 taccccgggc caggccacca aaccgctggt gaaaatgcgt cctttataac tctccttggg
     1261 ccgctgaata cagttggttg tcattaaaat ggcgccggga aacttgtcga actcctcccg
     1321 ctgatcgtgc caggcgccgc cgtagtttcc gaccagatgg ggatattgct ttagcttcgg
     1381 gtaggcgtgg gcggggatca tctcgccatg ggtgtagata ttgacactct ttcccgctgt
     1441 ttgctttaac agttcctcca gatccttcag atcgtgcccg gagatgagga tcgccttgcc
     1501 ctgaaccggg gtaatccgga ccggagtcgg aaccgggtgg ccataagttt cggtgttggc
     1561 ctggtccagc atctccatga ctctcaggtt aaccttaccg acctgcattg cccggtccag
     1621 gagttcggtt tcagtgaagt catcccgggt caggaagtcc aggatttcgt aacaaaaatc
     1681 atagaccgcg tcgtcttcga cacctaaaat ccgggcatgt tcggcgtagg ccgccattcc
     1741 tttcagacca aaactgacca gatttttgag gccgaaacga tccgggccca actgttccct
     1801 ttccagctcg ggccggactt gctcagcctg tttgaccagg ccttccagat tggtggccgg
     1861 aacccactcc gccgggcccc cgagtctttc cgggattccg ccattttggc gagactccgt
     1921 ttcatataat ttacgggcca cttctttcaa ggccccggcc cgttccaaca gactccggag
     1981 gcgatccgga tcgaaattga cattggtaac ggtggtgaac aacgcttcca gtagaaaatg
     2041 gtcgatcttg cggtcccgtg cccgggccct gtgcatatac atggcaatcc cggcactggc
     2101 gtggagcaac aaatcctgca aggcggcggt tgtatgatcc ttgccgcaaa ccccagccga
     2161 agtacagccg gtccctccgg ccgtttgctc gcattgaaaa cagaacatcc atctatcccc
     2221 ccttaacgtt tcggatttag cattaccggg aaggggggac aaaaattcaa aaaaaggcaa
     2281 gaggcatgag tagcgcgccc cgtggggcgc gggcaacagg ccctcacccc tcagatcatt
     2341 cgaagaagca gccctctcct agtcccgcct atttttggct tcaattcacc gtcttgatcc
     2401 ttcccacgca atcgcggggg aagacgggcg tcttgaaatg accctaggga tgagggcctt
     2461 aagcgttcgg cctagtcacc gcttacccct ttcttcccct ctccttattg ttttttagaa
     2521 ggagagggga agaaagggga gaggcaccgg aaggattttg attttggctc cgcgaatatt
     2581 ttagtttaga ggtttttatt ctttgccggg aggtttctgt atggtccata tctttaccga
     2641 taccacttct acgctgacac ttgaggaatt caaaaaatac gatattcatc ccattcccat
     2701 catcatcaat cagggagagg aatcccggcg ggagcttttt gatattacgc cagaggagtt
     2761 tttcaagaat cagcgggccg gcgtcaaata caccacctcg caaccgtccc ccgccgccta
     2821 ccttgatgcg tttaaaccaa ttatcgaagc gggtgacgag atcgtctgca tcatcttttc
     2881 cggcgggatc tccggcacct ttgacaacat ctactttacg gctcaaacat ataacgccga
     2941 tcggatcacg gtgttcaact ccggtctaac cgctttctgc cttgcctata tggcaattaa
     3001 ggcccgggag atggcaggcc gcggcgcgag ccgcgccgag atcatcgcgt atctggaagc
     3061 gctacattcc cgctgccgga tatattttct cgtggaatcc ctccactatc tccatgaagg
     3121 cggccgtctc tccggtgccc aggccctaat cggatccatc atccagatca agccgatcat
     3181 ctggtttgag ccggatggca aaatgtccgc attcgaaaag atccggacca ccaaagccgc
     3241 caagaaccgg ttgatcgaac tgaccaagga acgggccgac atcggaattg aagcaattgc
     3301 cctgcattat ggcgataata ccgccgaggc tactgaatat gcccgggaaa tcgaaaccgc
     3361 cgtcggaatt aaaccccatc tcattccgat ctccccggta ctctgcgccc ataccggccc
     3421 cgacatcctg gggatctgta ctgtcaccaa ggaataacaa ttatctatca ggaggtccct
     3481 tatgattaaa attgttaccg atacaacttg cgccatgacc ctcgacgaat acggtaagta
     3541 cggaataatc gcggtgcctc tctatgtcag acagggcgag gatacccaaa gagaattatt
     3601 cgacatctct tatgatcagt tttataaaag ccaacggacc ggaacgaaat ttaccacctc
     3661 gcaaccggat cccaattcat ttcttgaggt tttccgcccc attgtcgaag ccggtgatga
     3721 aatcatctgc gttaccctct ccggcaagat ttcgggcacg atcaactccg tcaccctggc
     3781 cgcgcaaatg ctcaactcag ataaggtaac cgtgatcgat tcaaagcaga gcggtttcgg
     3841 tcaggcttcc atggccctcc gggcccggga acttgcaaac tccggagcta gccgtgaaga
     3901 tatcgtaaga gatttggatg atctccagtc ccgcacccgt acttatttca tggtcgaatc
     3961 cctccgctac ctatatgaag gcggccgtct ctccggtgcc caggcgctga ttggctccct
     4021 gatccagatc aagccaatca tctggttcga cgccgaggga acaatggagg cgattgaaaa
     4081 gatccgcacc ctaaaagcgg cgaaaacccg taccatggaa ttgattcagg agcgtgtagc
     4141 ccgtggtgtg gaaaagatag cccttcatta cgcggataat tacgatgaag cggtcgagtt
     4201 cggcaagatg atggaggaga ttgccgggat gccggttcca ttaatcaagc tttccccggt
     4261 gttaggaacc cataccggtc ccgatctcct cgggccgtgc atcatcagta agaattaaat
     4321 tggggtcgat tccgggccta aaaccacccc tcttccatcc tctccccggc gatgttattc
     4381 attgaagttt attcgacggg gagaggtgaa tctgcctagc atcagggtca ttttcaggtc
     4441 ctgatcagca gatctaatgc tcaagtttca aacctgcacc tccctctccg tgaatccacc
     4501 tgcgaggtga atctgtctgg catcagggtc attttcaggt cccgatcagc agatctaatg
     4561 cccgaatttc aaactccaac ctccctctcc gtgaatcagc tactttgaat tagcttcgcc
     4621 ggggagggcc ggggtggggt ggttttaggc cccactgcta cttttatact ccgtctctcc
     4681 tcagcaaatc cagttgggat cgttttgatg attcacatac ttgcagaatt cgtttgatta
     4741 catggtagat gtcattttcg atttcgtcat tacgaaaccg aattacttgt aaaccccggg
     4801 atgcaatgat ccgatctcgc tcagcatcgt actctttata gagttcgtga attcctccat
     4861 ccgcttcaac caccactcct gcagcgtgac agtaaaaatc gacaataaag ccctctatca
     4921 cctgctgtct tctaaaatgt aacccgtgca gttttttatc tcgtaaatag aaccagagcc
     4981 ttttctcagc aggtgtcatc ttacgacgca attgtttagc ccattcttgt ttgataggct
     5041 taacattttg accaacaaca atttgctttg ctttcatgat ttctccttga acgactagct
     5101 ccagaatcgt tctttaaata ctcacccatc tattttccgg atggccgcac tgtgggtcga
     5161 ttccgggcct aaaaccaccc ctctccccat cctctccccg gcgatgttat tcattgaagt
     5221 ttattcgacg gggagaggtg aatctgcctg aaatctgagt tatttcggac actgattcac
     5281 cgaaatgatt caaacaaagg ataagaacct gtacttcctt ttgggatcca gaaccgtctt
     5341 tcaaagatgg atatcagata cctaagttta aaacccgaac ctccctcccc gtgaatcagc
     5401 tactttgaat tagcttcgcc ggggagggac ggggtggggt ggttttaggc cccactgcta
     5461 cttttatact ccggctttcc tcttgcatgc aactacttta aaaaccggcc tagagggaat
     5521 gagaaatatt ggttataaaa ttccgtattt tttttattat accttcaaaa aaataaagac
     5581 cccgaaacac tcgtccggag gtctttgatt gcctgcttcc atgattttag cctatctatt
     5641 cgaacaactc ccccatgagc ttacggatct ttttctcctc cgtagctgtc gcccgggccg
     5701 ccacagaaat ctccacgaca tccccttcct taatatcgga aggcaataaa gtcttggaaa
     5761 gccgccccat cttgcgatcc gacagctcca ccagtgcata atccccttca aaacgatcga
     5821 tggtaacctt catgattcat tcacccgcct tgtagtggac ggtatttccg tcgctggtaa
     5881 agatgatggt ccctgcctga tcggtcctca gaactttaat tccggctttg ttcagccttt
     5941 ttaaggtgga tttgccggga tggccgtaat cattccccgc tcccacggag atcacggcat
     6001 agctaggccc caccacccgt aaaaaccccg tcgcagtaga ggaattactc ccgtgatgtc
     6061 ctaccttaag tacgtccgat cgcaaatcat atcccgcccg gagcatctcc cgttcggaag
     6121 tgaatccggc atcgcctgca aagagaaaag ccgttctgcc ataaactatc cgaagtacag
     6181 cgctgtaatc gttcaaattt tcataagatt cccggttggg ggcaaccaat tccagcttga
     6241 gcgccgggtc aagatcgaag cttaatccgg cacgggcggt ggtgatcttc agtcccttgg
     6301 ctttaattgc cagcaatagt gattcaaagg tccgggtatt ggttgcggcc ttcggcatac
     6361 tgatctgatt gatactgtat ttttgaatta ccgcatccat tccgccgata tgatcctcat
     6421 gaggatgggt ggcgatgacc aaatccagtc tactgatttt ttgggcggta agatatgaca
     6481 aaacccgttc ggcggattcg ttagttccgg catcaatcaa gatattccgg gaatttgggg
     6541 tccgaatcag aatgcaatcc gcctggccga catcgaagaa ataaacctta agcggctgaa
     6601 gcgccgcgtg aacaggagta aaaacaacgg ccgcaaccag cagcacctga cataggccca
     6661 aaaccagccg gcgatggaat gaatgcatag taaggcttcc cttcgtgttt tacgatagta
     6721 cattccggtt catattcgaa cagcaatgtt ccggtaccgt gatacccttg acaacggtac
     6781 ctacagccat tatgaacccg gcatcgcttt tcgtcaaaac aaatatttcc tgtagttaaa
     6841 aagaatttat gcctgcgact cacccctagc ctcgcgccca atgggcgtgt cctaaaggac
     6901 acgcctctcc cggcaagcgg caaaaagggg agaaggcaga ttcaaagcag taaaatagac
     6961 tacgaaggat tgtggttact ctctctggcg cttgccgcta gagagagcaa gagtgagtcg
     7021 taggcaacga agccgactca cccctagcct catgcctatt gcctgttccc tgttgcctat
     7081 aataaaaaaa ggccggaaga gattccagcc ttcaacttct attcaaaaag cgtccgcatg
     7141 agtttgacga gtctttgcat gttcctgcgg gtgccgccgg aatcccggcg cagaatcaac
     7201 cgttcgccct cccggatccc ggtaccgcaa acccgcaacg gcaggttgac cacggttcgg
     7261 tcggaaaatt ccaggaccgc ataagaaccc tcgattcgat cgaccacagc cacagccata
     7321 acctcttccg gataaacccc catccggata acggcgatta ttcccatagc cagcaagatg
     7381 acagccccga tccaaaagcc tcgcaagcgg tagtccatct ccggcaacct ccgcgcctca
     7441 agtatcccct ctcctgaaca tgccttgtcg ccagaattat atcatatttt tatatattcg
     7501 ttaaatgtcc ataggatccc tctattcgcc gcctcataca acgcatccct ataggttgcg
     7561 atgcctttgt tgcctgttgc cccgcgcctc acggggcgcg ctactcatgc ctcttacctc
     7621 gcaccctaat ctgtcgggaa tttccggtgt cttcgaagaa gcttgaaccc ttcaaaccag
     7681 gcgatgctta aaatcccggc agttaaacag attgccagat caccggcatg caacggcgcg
     7741 aaacggaaca aactcctcaa gaacggaaca ttaagcacca atcctaagaa gaagacggct
     7801 ccgcctgtaa cccaccaaag agcggtattg ggggtccgca ggctcttaaa aatcgtctgc
     7861 gaccaggaac gattggttaa aatcaacccc aaattggcca cgattaatgt ggcaaaggtg
     7921 aaactccgcg cctcggcctc ggaaagttcc cggttattga tggctatcgc gaagacgccc
     7981 aacacgatca caagtacgct gaacccctgc atcaggccga ccagtaacgt tctgctgctg
     8041 aataacggtt cctttggatt gcgcggaggg cgctccatta cgtcagcctc ctcaggctcc
     8101 gcttcaaaaa ccacggaaca ggccggatcg atgatcaact ctaaaaagac aatatgcacc
     8161 ggaagcagta tcagcggcca tttaaagagt accggaatca gcgagagacc agcaatgggg
     8221 acatgaaccg aaaaaatata ggtcatcgcc ttttttagat tatcgaagat ctttcgtccc
     8281 tgacgcaccg cagtcacgat cgacgcaaaa tggtcatcca gtaagactag cgccgacgcc
     8341 tcgcgggcca catccgttcc ccgttcaccc atggcgattc cgatatgggc ggatttcaag
     8401 gccggggcat cgttgacccc atcaccagtc atcgccacca cctcaccccg ggcctttaaa
     8461 gcgttgacaa tccgtaattt ttgctccggg accacccggg cgaagatgct tacctccgcg
     8521 atgcgctgtt gtaactcctc gtcacttagc gcctcgatct cagggccggt gatcaccatt
     8581 tccggtttgg caagcccaat ctcctttgca atggactggg cagtgcccgg ataatccccc
     8641 gtaatcatcg ccactcggat tccggcccgg tagcattctt gaattgcctg cggaacagtg
     8701 gcccgcaccg gatccaccaa accaaccaag cctaagaatt gaaattgaaa atcatgttgg
     8761 cctgaaggta gttgtgactc ctgaaacgaa gcccgggcca ctcccaagac ccgcagtcca
     8821 tcatctgcca tccggcgtac cttttcattc aagtcttccc ggacagacgg ttcgagatgg
     8881 cagagatcgg cgatggcttc cggagcccct ttggaagcga tgacataatc cacaccatct
     8941 ggagatttcc aaacatggga tagggccagt aactccttgg aaagcgggta ctcctgcact
     9001 aaactccagt cgtgatggag atgttccgta ttggaaagcg ctgaatcgcc gacttgcctt
     9061 aacgcccgct ccatcggatc aaacgggtct ttctggcttg ccaggatcgc gtactccacc
     9121 agcttatgaa atccctcggg taacggcccg gtccccgcgc catttaaagt gaaaaaacgt
     9181 cccccggcat aaagccgttt caagaccatc tggttgcgtg tgagcgtgcc ggtcttatcc
     9241 acgcaaagca cagtcgcgga tcccagtgtt tcaacaaccg cttctctgcg ggtcaaaacc
     9301 ttcttttggg acatccgcca tgcccccaga gccagaaata cggtcagcac caccggaaat
     9361 tcctcgggaa gcattgccat tgccaaggta atcccggcaa gcaggccatg taaccaattg
     9421 cctctggtta ggccgaagac gatcaccacc aaaagacaga gaaccaggcc caaaacggcc
     9481 agattccgca ccaaccggcc aatttcctgt tgcaacaagg tcttttccgt gccaattgtc
     9541 tgcaaggctt tgccgatctt gccgatctcg gtactcaaac cgatcccctg aaccacagcg
     9601 atgccctgac cctgaactac caaagtgcct gaaaatacaa acggcaaatc gtcaccgccg
     9661 ggcgccccca tcggctgaat ctccttaact gcgttcttcc ggactgccac cgattctccg
     9721 gtaagcaacg actcatcaac agccagattc agcgaatcaa tcaagacggc atctgcagga
     9781 acccgatcac cttccgccag aataatcgta tcgccgcgta ctacctcccg gccggggatc
     9841 cgttgccgtt cattatcgcg gatcacaagg gcgcggggtg acgaaagatc ccgtaacgct
     9901 tccaaggcac gttcggtttt acgttcctga aaaatggtga tccccatgac cacaaatacg
     9961 aaccccagta acattaaggc ttcgccaata tccccaagca gcagataaag actgccgcaa
    10021 gcaacgagca gcaggaacat cggttccttg aagacctcca gtgcaatccg gagaatactc
    10081 cgctttccgg ccgtcggcag ctcattgaag ccctcccgct cttgtctctc cttgacctcc
    10141 cgtgatgtca aaccggtgat acccgcgaaa ttcattaaat tacccattcc atcgcccccg
    10201 catccgttcg ttttgggtat aaaaaaaccc gtggaaccgt tttggtccca caggtcttgg
    10261 ttgattccca attaacctgc tgctaaaaag cccagcccca ccatccccta cgtgtgcttg
    10321 tttccgggat gatcgcctgt tccgaaagac tcaattcgga agatacctta gaaaaaaagt
    10381 gctggcacac agataattta tcattaaaat tcggaattgt caatcatttt atttttgtct
    10441 cccctcaaag gttggttcat tcggcctcca aaccgcatct atcggcatac cgggccatag
    10501 gaacccatct tgagactttg gtttggtccg gcttgccgcc ggccattaat atattgtagg
    10561 taagaggagg tctaccaatg tctgtgatga tggttcagat tatcgaccgc cacagtcaaa
    10621 tgcaactgcc gcctcaggac gaaagcaatt gttcctatcc gaccatcgcc tatcattgca
    10681 cccgggaaat ccaaggaccg gagctaatca agcggtgggt tgacctgatt agaaataccc
    10741 ggcggcatta atatgggaat tacgactttg cttgcgattc atcaggatta atcgaaaaaa
    10801 agagctggtt tgatcaagca accagctctt ttttatggat tgtttgtgat cttcgcgagt
    10861 tactggacaa acttcaagcc ggtcaaaata ttctccaaca tcttggcctc atcttccgtg
    10921 gtataactaa tcacgaagga gatggcgtaa cctttccgga ctgtagcgta gtatctttgg
    10981 tatacggtca tttgggcaat actgacttga gtttccaggg tatcaaaatt tactccgccc
    11041 agcacttgcg ggtagatctc cttgttgaac tgaaactgga cttgaccgct ctccataatt
    11101 cttttgacat tcaataaata atctttgccg acctgaattc ccggataaag ctctaccttc
    11161 tccgccaccc aaaccatgct gggattgtac tccacctggg tgccaagggg ataacggaag
    11221 acagtcagca gcactagtga actctgttcc gccgcatcca gcgcagcttg gactttctta
    11281 tcctcatcgg gcaagacttc tttaccactt tccagcattt gtttgacgac ggcttcgcct
    11341 tggacctgcc aatccccggg aatcggtaaa gacatcccca aatagtcgtt ccggtaaacc
    11401 gaattttcta tcgaaccata atccatcacg gcaggtttcg tctccgccgc aacatccgct
    11461 gtggcctccg gatcagcatc agtacccgcc gggacggcgc ctgtctcatc cgctgcccaa
    11521 accatgccgg ttccaggcat tgctacgaga aagaaacaga tccacccgac gattaaaaca
    11581 tttcttttca tatcaggaaa acctcctcta tcctcccaaa cattatatca taatccgagg
    11641 agcatcataa ttttttacga tttgttttcc tttcacggca ggtaaacctg ggattaaagg
    11701 cgaaaacagt ttaaaaaaga catctcttat ggggtattct tggaagcaca cttcgtcgga
    11761 taagccggcg ttttgacaat ggagtaaaca gatgctgatt atcggaattg ccgggggaac
    11821 cggttcgggt aaatccaccg tagcagaggc gttgcaaagg gaactcggcc cggaaaatgt
    11881 ggccttgata tcccaagact cctattacgc ggatcttgcc gatttaccct ttgaggaacg
    11941 ggccaaacgc aactacgatc accccgacgc ctttgagaac ggactattga tcgatcactt
    12001 acgccggctt cgcaatgggg agacggtcga ggttccggtt tatgactttt cacaacacgc
    12061 ccggacctca agaaagattt cggtcacacc ccgcccggtc attgtggtgg aagggatctt
    12121 aatcctgatc gaagcggcgt tgcgaaccga gttcgatatc aaagtttatg tggatacaga
    12181 cgctgatacc cgggtaatcc gtcgaatcac aagggacatc gaagaacggg gccgcaccct
    12241 ggaatcggtt tgccggcaat acttgaatac ggtcaagccg atgcatgaag ccttcatcga
    12301 accgtcaaaa cgttacgcgg acatcatcat ccccgtcggc ggatacaaca cggtcgcctt
    12361 gagcttgctt gtctcgagga tcgagagtta tttgcagagt atcagtacaa atgattgaaa
    12421 aacttgattg catatccctt gtttatgact taggtatata acatattaat ttttttctga
    12481 gttatttgat attaatatct tacttttgcc tccatggccc ctctacaaat atctcccaat
    12541 tagtcgttat tttcccattt attcttttac ttgctctcgt tgaatgatta aacaactctt
    12601 ttaagcactt tacatggatt atggtaagtt gaaataatct taattcattg ctatgattaa
    12661 aatgtattta ttgtttacgt taggaggcat agcaatgaaa aaacaattat tccttcttct
    12721 cagcttttta cttataaccg ccatagtttt atctggttgc ggcgggggtt caccttcagt
    12781 ccaagtaaaa acaatcgaat atcaggatga tggaaatggt ttcactcagt tttacacaaa
    12841 cgataaacga aattataatt atagtttttg gcattcggat ctaacacctg aagaaactac
    12901 gtttccagta gaaattcaag tcaaaaaact ttccggaaat aaaagtatgg tccatggtgc
    12961 atttttttgt tataaggacg atcataactt ttatagggta gtaatcgata ccgaaggaaa
    13021 ttactatatt tttaaacaca attcgaatgg cactgaacct aggacaacaa tacgaggaaa
    13081 gaccgcatcc cccaacctaa ataccggtta taatacactc aacaccatcc atgttgagcg
    13141 tgatgatgaa ggccaattca caataaaatt taataataat tataaaaatg acaccgaggt
    13201 aataacattt gaagatacca catttaccgg tggcaggtgt ggtttttatg ttgctgtcgg
    13261 tgatgaaacg caggaacagt ttccgaatac tcccgtcgac gtaaggttca aacagatatt
    13321 accggaaggc gcttcgatct aatctttccg atgtacattg ataataagtt tgcattgatc
    13381 gagtaggagg gggcggctag cccccgtcct ctcacaccac ctagcatgcg ggtccgcact
    13441 aggcggttcg acagttttga cgaagtacta agtatctttg agttaagctc ataagccctt
    13501 gggattgcca gtaggctttt ccgagggctt tg
//
LOCUS       DASVMB010000084         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_157441,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000084 DASVMB010000000
VERSION     DASVMB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157441"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VF531_02770"
     CDS             <1..270
                     /locus_tag="VF531_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HEX7712921.1"
                     /translation="ADLGGNLSAAAHLIHGSSEGRFSITYAPGHLTEAELKQANLGYL
                     PLEEAIRRYRPEQLQNGMNRLADGTEFFYISNPALGLWALRKNFE"
     gene            642..1841
                     /locus_tag="VF531_02775"
     CDS             642..1841
                     /locus_tag="VF531_02775"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX7712922.1"
                     /translation="MLILVLNSGSSSLKYKLYKMENEEVLASGLVERIGSSNGNAVIN
                     HQVGGGDKIRLEQKITTHNEALRAVLDFLTAPGQGVIKSLNEINAVGHRVLHGGEIFS
                     SAVIVGEKELAQIESLRELGPLHMPANIMGIKACQELMPQTPQIAVFDTAFHQTMPRS
                     SFLYALPYEYYEKYRVRRYGFHGTSHRYVSQRAAQILGKNLAKLKMITLHLGNGSSLA
                     AIKDGKCLDTSMGFTPLGGLVMGTRCGDIDPAIVPFLENALKLSPSEIDSLMNKKSGF
                     LGMTGFSDMRDIQKARAEGNERAQDVYGVFIHELVKYIGSYYAILGGLDVLVFTAGIG
                     ENDWIIRADLCEKLTHFGVEFDFKKNENLRGKETIISKPRSKVTVMVVPTNEELLIAR
                     DAYALVK"
     gene            2042..2524
                     /gene="smpB"
                     /locus_tag="VF531_02780"
     CDS             2042..2524
                     /gene="smpB"
                     /locus_tag="VF531_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7712923.1"
                     /translation="MAERKLERKNVAMNRQARHNYFIQETYETGIVLTGTEVKSLRQG
                     KGNLQDSYAQITGNEIFLYNCHISPYDFGNRFNHDPLRARKLLMHREEIRRLFSKVRE
                     QGLTLVPLTMYFDERGRVKLELALAKGKKLFDKRQDMAKEDATREMARAMRDRQKDSY
                     "
     gene            2530..2882
                     /gene="ssrA"
                     /locus_tag="VF531_02785"
     tmRNA           2530..2882
                     /gene="ssrA"
                     /locus_tag="VF531_02785"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          954 a          780 c          847 g          874 t
ORIGIN      
        1 gccgatctgg gcggtaatct ttcggctgcg gcccatctga tccacggttc gtccgaaggc
       61 cgtttctcga ttacatacgc cccgggccat ctgactgaag ccgaactgaa acaggcaaac
      121 ctgggttatc tcccgcttga agaggcaatc agacgttacc ggcccgaaca gttacaaaat
      181 ggtatgaacc ggctggccga cgggaccgag tttttttaca tcagcaaccc ggcgctgggg
      241 ctttgggctt taagaaaaaa ctttgagtaa aaaatggaac cgtatgatga aatgcattcc
      301 gtgcttgcat gcgtggacgc taaccggtgt caccttaggg tggcactttt ccattttttt
      361 ggatcaaaac gaacgttttt tgagtatttc atttcaaatg aagttatttc agcaacaatt
      421 tattaacatt atttcgttgt aaataagatt gcaaacattt ttgggcctga ctataataaa
      481 agaggctgtg attcaattgg ttctgttttc tttggtagaa catcaatcat ggctttccaa
      541 tgttgattga atggattcag caagccagac ggagcgagaa tcagtctgtt acttatcata
      601 gacagatgat gcaaaaaata cgattgagga ggcgcaagct ggtgctgatt ttagttttaa
      661 acagcggaag ttcgtccctg aagtacaagc tttacaaaat ggagaacgaa gaggttctgg
      721 cctcgggtct tgtagagcgg attggcagta gcaacgggaa tgcggtcatc aaccatcagg
      781 taggcggcgg cgacaagatt cggttggaac agaagattac cacccataat gaagccctaa
      841 gggcagtgct tgattttcta accgctcccg gtcagggagt catcaaatcc ttaaatgaaa
      901 ttaatgccgt cggacatcgg gtgctgcatg gcggtgagat cttctccagt gccgtgatcg
      961 tgggcgaaaa ggaattggct cagatcgaaa gtttgaggga actcggaccc cttcatatgc
     1021 cggccaacat tatgggaatc aaggcttgtc aggaattgat gccccaaacg ccccagatcg
     1081 cagtcttcga taccgctttc caccaaacca tgcctcgcag ttcgttcctt tatgccttac
     1141 cttatgagta ttacgagaaa taccgcgtcc gccgttacgg atttcacggc acttcccacc
     1201 gttacgtttc ccaacgggcc gcccagatcc ttggcaaaaa cctggcgaaa ctgaaaatga
     1261 tcaccctgca tctggggaac ggcagcagtc tggcagcgat caaggacggt aaatgccttg
     1321 atacttccat gggtttcacc cccctggggg gactcgtcat ggggacccgc tgcggcgaca
     1381 ttgatccggc gatcgttcca tttttggaaa atgccctgaa acttagcccg agtgagatcg
     1441 actccctaat gaacaagaaa agcggctttt tgggcatgac cggttttagc gatatgcgcg
     1501 atattcaaaa ggcccgggct gaagggaatg aacgggctca ggatgtgtac ggtgttttca
     1561 tccatgaatt ggttaaatat atcggctcat attacgccat tctgggcgga ttggacgtgc
     1621 tcgtcttcac cgccgggatc ggcgagaacg actggatcat ccgggcggac ctctgcgaaa
     1681 aacttactca tttcggagtg gagtttgatt tcaagaagaa tgagaatctc cgcggcaaag
     1741 aaacgatcat cagcaaaccc cgttccaaag tgacggtaat ggtggttccc accaatgagg
     1801 aactgctgat cgccagggac gcctatgctt tagtaaagta attgattatt tagacatgaa
     1861 aatggaagat ttaggaagac taagagctac ccttgggtag ctctttttgc atgcaaagca
     1921 cccaggcttt cctgcgggtt gacttttcac cgatccatgt tataattaca tagcaatgac
     1981 tatcttgccg caggtttaac cttgggttaa accagaggtt aagcggaggt ttttgggaac
     2041 gatggcggaa cggaaactgg agcggaaaaa tgtggccatg aaccgccagg cccggcataa
     2101 ctattttatt caggaaactt acgagaccgg aatcgtcctg accggaaccg aagtcaaatc
     2161 gttgcggcag ggcaagggca atctgcagga cagttatgcc cagatcaccg gaaatgagat
     2221 cttcctgtat aattgccata tcagtcccta cgattttggc aaccgcttca accatgatcc
     2281 gctccgggcc cggaagcttt tgatgcaccg ggaggagatc cgcaggttat tcagtaaagt
     2341 ccgcgaacag ggactaactc tggtaccgct cacgatgtac tttgacgagc ggggccgggt
     2401 caaactggaa cttgcgctgg ccaaagggaa gaaactcttc gacaagcggc aggatatggc
     2461 caaggaggat gccacgcggg agatggcccg ggccatgcgc gaccggcaga aggatagtta
     2521 ttagaaaacg ggggcgaccg gtttcgacgg ggagtgtggg ttaagaggta gcgggtcgag
     2581 gtgctgtggc ctcgttaaac tgcagcaagc acattaagtg ccaacaatca agaattagct
     2641 ttagctgctt aattaagtag ctcgtccaat ccgggcttaa cctgggaggc cggtgcgggc
     2701 gtcaaataaa ccaggacgct cagcagtcaa ttctcggaga ctgttgctaa gattatcgag
     2761 atagccaagg cctagtttga ccgtagacgc ggcggcggcg aaatctaaat tacggtctgc
     2821 acccggagcg acgtcttagc ttaccttttc ggacgggggt tcaactcccc ccgcctccac
     2881 caaaaattaa acttttataa gaggcgaacg cctccaccaa ttttaaagaa aataccagct
     2941 aaaggctggt tttttaattg gaaaaaatca gtcctccacc aaatgtgaac taacttagat
     3001 tgattacctc caccaaaata aaatccagca tccgtttaga atgtaatata ccgttttttt
     3061 gaaatatgtt tgatcctcct cgtgaaatat tttctataca atcatttcca attactctcc
     3121 aaatccttaa actttcaagg gttttttctt tttcactcaa aaatccgtta agtggtggtt
     3181 aagtggcaac tggtataata aaattaaccg attgtgaaac tattctaatt ttagaggttt
     3241 cttcagttta aatattaata tccatgccaa ggaagtgttt ctaaatgaaa aaatttactc
     3301 ctcacatttt agtaactcaa ctttcgcaga tcaaattagg caaataaagc ttgatataat
     3361 tgggtaaacc ggcaaaccaa ttaagaagtt gacaatcaat gaatttacgg atcttttggc
     3421 aaacattcgc tgaaatatcc gctaacaaat ccaaa
//
LOCUS       DASVMB010000085         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_452708,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000085 DASVMB010000000
VERSION     DASVMB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452708"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1204)
                     /locus_tag="VF531_02790"
     CDS             complement(95..1204)
                     /locus_tag="VF531_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712924.1"
                     /translation="MRKIQIAILALCLSFGFSFTAYGLETSGYLENQLQITDGDNLFD
                     TNKLRLDFQVKESGYYMLVSVNALNGLMQGDISLQNADLSDPNVLLSLENNLLELSRA
                     YLELYPSWGKVTLGLQNIAWGNGYLFNLPDQFNPPNPLDPKGEKQGINAISTKWNLNG
                     TAQLEAVAVPAAKLSGTDYGVKARVNIGLFDISTFFLKRYNIPVNLSQPTIRFDRTAG
                     VLEIKGELGDGLPGIWLQGGWFGDTNLATNSTENYFTYVTGSDYTFNVGNGLYCLAEY
                     MRNTQIEANQVYFLTRYSPQGYLTFSLSGMRNNQADAAMYSLNSEYQLNDNIEITGMY
                     NVYPQGSSYFTMGNGTLQNNAGFTSQIVIGVKTTF"
     gene            complement(1227..1814)
                     /locus_tag="VF531_02795"
     CDS             complement(1227..1814)
                     /locus_tag="VF531_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7712925.1"
                     /translation="MSKDETRQRIMTMARTKFFSLGYSSLTMDELAADLGLSKKTLYK
                     NFASKNDLLKEVVEEFMGEQIVQVQQILNSTESTMEIMKRLVMFIGNLASQISHLRIQ
                     DLQKNAPEIWKEIAEVREKYIAANFKNILQDGIREGKFRSDVNPDLILMILISCINGI
                     INPITLAQLPISIREAFEGIITVVFNGILTEGNRI"
     gene            2236..3078
                     /locus_tag="VF531_02800"
     CDS             2236..3078
                     /locus_tag="VF531_02800"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX7712926.1"
                     /translation="MPNRQIAAWIIGTALTLSRNSQFGIDPGFIDAEEPGETLEKIII
                     LGDETMGNELETAFERIEQLLYSCENGTNLDLAETALTKIKNEANSPQVLAKVYGKLA
                     QVENYRYEYVSSGEKLAVAEKGVNLAKKGLEYDENNVDANSWAAAMMGIHGMEMGILS
                     SLFYLGPVKKHAEKALQLDESYHYGQPHIILGELYRLSPPSPVGMRNLNKALEHLRRS
                     VELGPEDPQARFHLAELYINLRRKELAFKELETLLSQDIQVRGPVYAENWKAKAGKLR
                     EKLA"
     gene            3115..>3941
                     /locus_tag="VF531_02805"
     CDS             3115..>3941
                     /locus_tag="VF531_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HEX7712927.1"
                     /translation="MTTVSVTKSLVPRLEAMRRRVLEAPQEVSLIRALAITHAVKSNP
                     GMPREIQLALGFRAAMNQLPVSIAEDERIVGSLTEKFKAAMLYPELKSDFIGKEVNNF
                     EEREKDRFFLNDEEKRLIREEIAAFWNGKNGYDSMTSMLNGDTDFYVRNLAMVIMNDF
                     MGANHLSHIEYEKVLSRGFNGILREIEAAVQNLSESDPEFLQKKNFYQSMIISIQAVN
                     DYAARYAALALKMAGDAATAERTRELTAIADMTSQVPAGPARSFREAIQAFWFTVIA"
BASE COUNT         1046 a          878 c          971 g         1046 t
ORIGIN      
        1 tggccatttt ttgttccccc ttaaggtttt ccatttcggc ttccactttg gtaaaaccta
       61 aattggcgtt tccattattt atccatgaat cccgtcaaaa tgtggtcttc accccgatga
      121 cgatctggct cgtaaagccg gcgttatttt gcagggtgcc gtttcccatg gtaaaataag
      181 agcttccctg cgggtaaaca ttatacatcc cggtgatttc aatattgtcg ttgagttgat
      241 attccgaatt taagctgtac atcgcggcgt ccgcttggtt attccgcatt ccgcttaggg
      301 aaaaggtgag atacccttgt gggctgtagc gggttagaaa atagacctgg ttggcttcga
      361 tttgagtatt ccgcatgtat tctgccagac agtatagtcc gtttccgaca ttgaaggtgt
      421 aatccgaccc ggtaacgtac gtaaagtagt tctccgttga gttggtggca agatttgtat
      481 caccaaacca tccgccttgc agccagatcc cgggaagccc gtctccaagc tcgcctttga
      541 tctctaaaac cccggcggtg cgatcaaacc ggatagtcgg ctgagataga tttaccggta
      601 tgttgtatct tttcaagaag aaagtggaga tgtcaaacaa accgatattc acccgggctt
      661 tgactccgta atcggtaccg gagagctttg cagcaggaac tgcgaccgcc tcgagctgag
      721 ccgtgccgtt aagattccat ttggttgata tggcgttgat tccttgtttt tcccctttgg
      781 gatccagagg gttgggggga ttgaattgat cggggagatt gaataagtag ccgtttcccc
      841 aagctatgtt ttgcaggccg agcgtgactt ttccccagga aggataaagt tccaggtaag
      901 cccgacttag ttccaacagg ttattttcaa ggctgagaag tacattggga tcgcttaaat
      961 cggcgttttg cagggaaatg tcaccttgca tcaaaccatt cagagcgttc accgatacca
     1021 acatataata accgctctct tttacctgaa aatcgaggcg taacttattg gtatcgaaca
     1081 gattgtcacc gtcggtgatt tgcagctgat tctccagata tcctgaggtt tcaaggccat
     1141 aagccgtgaa tgagaaaccg aatgataaac atagggccaa aattgcgatt tggatttttc
     1201 tcatttcaaa tccctccttg aaagaattaa atccggttgc cttccgtaag aatcccgtta
     1261 aaaacgactg taataatgcc ttcaaacgct tctctgatcg atatcggcaa ttgggcaagg
     1321 gtaatcgggt tgataattcc attaatgcaa ctaatgagga tcatcagtat caaatccgga
     1381 tttacatcac tgcggaactt tccctcacgg atcccgtctt gcaagatatt tttaaaattc
     1441 gcggcgatat atttctcgcg tacctccgca atttctttcc agatctccgg cgcattcttt
     1501 tggagatctt gaattcgcaa gtggctgatt tgagaagcga ggttcccgat gaacatcact
     1561 aaacgcttca ttatttccat ggtagactcg gtgctgttta aaatctgttg gacttggaca
     1621 atttgctctc ccataaactc ctcgaccact tcttttaata aatcattctt tgaggcaaaa
     1681 tttttgtaaa gtgttttttt gcttaagccc aaatcggcgg cgagttcatc catggtcaga
     1741 ctggagtagc ctaatgaaaa aaatttggtt ctcgccatgg tcataatccg ttgccgggtt
     1801 tcatccttcg acaccaaccg ttttcaccct ctcccgctta aaatcccatt tagctcgaag
     1861 atactgtgga aacttttatc gtttccttag tttccattat atgcgatctt tcctcaggtg
     1921 tcaaatttct ctatgactct tatgcggtat tcagccagga taaacggtga agcagaacat
     1981 gttggattta gggcgggctg gggcaaaaag ggaatataaa aacactgttg ttaaatacaa
     2041 ctgttgtatg atacaatata gacatttgga aaggaagagc tgttgtgagc gggctggggg
     2101 cggaaataat gcttaggtca gggaaatacg tcgggttata gttgcagaac acaacagttt
     2161 gatgttgcaa caagaagagg tgaaatcatg cattctgccg cttctggacc atgatgtgtc
     2221 cgcggtggac agcctgtgcc aaatcggcaa attgctgctt ggataatagg tacagccctg
     2281 actcttagta gaaacagcca attcggaatt gatccgggtt tcattgacgc ggaagaaccg
     2341 ggtgagactt tagagaagat cattatttta ggagatgaga caatgggtaa tgagctggaa
     2401 acggcgtttg aacgaatcga acaattgcta tattcctgtg agaacggcac taaccttgac
     2461 ctagctgaaa ccgcattgac gaaaataaaa aatgaggcga acagccccca agttttggcg
     2521 aaagtatacg gcaaactggc gcaggttgaa aactaccgct atgaatacgt atcttccggc
     2581 gaaaagctgg ccgtagcgga aaaaggcgtg aatcttgcca aaaaggggct ggagtatgat
     2641 gagaataacg tcgatgccaa ttcctgggcc gctgcgatga tgggcatcca tggcatggag
     2701 atggggatac tgagctccct tttttacctg gggccggtga aaaagcatgc cgagaaagcg
     2761 ttacaactgg atgagagcta tcactacggt cagcctcaca ttattctggg cgagctctat
     2821 cggttatctc cgcctagccc ggttggcatg cgtaatctca ataaagctct ggagcatttg
     2881 cggcgttcgg tagaactcgg gccggaagac ccccaggcca gattccatct ggccgaacta
     2941 tacatcaatc tgcgccgaaa ggaacttgct ttcaaagaac ttgaaacttt attgagtcag
     3001 gatatccagg tccggggacc cgtttatgca gagaattgga aggccaaagc cggaaaactc
     3061 cgggagaagc tggcctgaag ttaaaataat caaccattat ttgggaggtt tatcatgaca
     3121 accgtttcgg ttacaaaaag tctggtgccg cggcttgaag ccatgcgccg gagggtactc
     3181 gaagctccgc aagaagtcag tctgatccgc gctctcgcaa ttacccatgc ggtaaaatca
     3241 aatccgggca tgccccggga gatccagttg gcgctgggat ttagggcagc catgaatcaa
     3301 ttgccggtat ccattgccga ggatgaaagg attgtcggct cgttaacgga gaaattcaag
     3361 gcagccatgc tctatcccga attaaaaagc gactttatcg gcaaggaagt caataatttt
     3421 gaggaacggg aaaaggatcg cttctttttg aacgatgaag agaaacggtt gatccgcgag
     3481 gagatcgcgg ctttttggaa cggcaaaaac ggctatgaca gtatgacatc catgctgaac
     3541 ggggataccg atttttacgt acgcaatctg gcaatggtga tcatgaatga ttttatgggc
     3601 gccaatcatt tatcgcatat tgaatatgaa aaggtgctga gcagaggctt caacggaatc
     3661 cttcgggaga tagaggccgc agttcaaaac ctctccgaaa gcgacccgga gtttctccaa
     3721 aagaaaaact tttatcaatc catgatcata tcgattcaag ccgtgaatga ttatgccgcc
     3781 cgctatgccg ctttggcgct aaaaatggcg ggggatgcag caaccgccga acgtacccgg
     3841 gaactgaccg ccatagccga catgacttct caggtgccgg ccggtccggc gcgcagtttc
     3901 cgcgaagcga tacaggcgtt ctggttcacc gtcattgcat t
//
LOCUS       DASVMB010000086         6067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_10589,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000086 DASVMB010000000
VERSION     DASVMB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10589"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VF531_02810"
     CDS             <1..691
                     /locus_tag="VF531_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7712928.1"
                     /translation="RIAFIGSEPHSEEMRKQIEKLYGIKAFNSYGLSEVCGPGVAFEC
                     PEQAGMHIWEDHFYIEIIDPRTGRVLPEGEEGELVLTTLRREAMPLLRYRTGDLTRIL
                     PDACSCGRTHRRIDRIKGRADDMLIINGVNTFPIQIEQTMMRIPGVGNNYSIEIRKEN
                     YMDKMYIQVEFDRDVFEGTLEELTNLQNRVAEELKSELVISPVVRIVEPGSLPVSEGK
                     AKRVHDLREKV"
     gene            709..1137
                     /locus_tag="VF531_02815"
     CDS             709..1137
                     /locus_tag="VF531_02815"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7712929.1"
                     /translation="MASHLSIFVENKPGKLEKLTRILAENQINLKAFSLASVGEYGIV
                     KIIVNDIDQAFEVLKKEQITVSKRRILIATVPDQPGGLHRLLTVLSANQINIQDCYGF
                     VIENRKEAAIVIEVEKYPLAEEILRREEIGIMADEDLGKI"
     gene            1337..2314
                     /locus_tag="VF531_02820"
     CDS             1337..2314
                     /locus_tag="VF531_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712930.1"
                     /translation="MKNHLKPMGVRDILDSTFTIMRENFWSLQGIFFKAFFPSLIILF
                     AGIIGAVVYLALLGTHTDIPITNPLFWSEAGHGSTSVIILGIVGLLLVALAFIVAAVI
                     GDIYYTYGVLKIFKNGLHDQKTSSKEAFRGVKGNRARIFWVQFVVGLLLYIVILPGVV
                     ISVNVNLLSPLTGTFISYANNFLQLVIAFFFCMTSPVVVYENLKVMKSIGRGIKLLSH
                     HRWRIFWTLLLVYLLGYALGLILLGFIAIPIVVAAMLKNMIAYIIAAIFGIGASFLLV
                     LLVAYFYGPLAAIYYDLIIRKEGYDISVQLAEAAQAGTEGQSTAQPASF"
     gene            2332..3078
                     /locus_tag="VF531_02825"
     CDS             2332..3078
                     /locus_tag="VF531_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712931.1"
                     /translation="MKKIILLWALVLMVFPAFVVSAAAAGTTLNNSDNRVRTAPPNIP
                     VLRRNIREILKDPRFNYPDRWKGLQRFWQNIRNWLAKLDSSKRSGKQMPWNKALRWIG
                     LAALAVLPLVLIYLLPKLFARSGRVKSLSPKTAVPVVRSEDLKVQARIMADKGQFREA
                     IRLLYLAGLEDLKTNGILPDGVRLTDKTNLNIVNRTFGSDHPCSQAFRELMLVFEEKW
                     YGLRSCQTEDYDRLTGYLKILEANTGKPHV"
     gene            3071..4210
                     /locus_tag="VF531_02830"
     CDS             3071..4210
                     /locus_tag="VF531_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX7712932.1"
                     /translation="MSKHKVIMGLVALGLLMGILLFLSWVSLSQKKLVAYDANSYGPD
                     GLKALDLILKKSGYQVTLVHSLPVNHQSLLILVTTTRLSAKQQKTILTWVSAGGNVLE
                     LASSWPSLTRESDILDVTAGRPFQSDAANLNLSGLTYHISKNWVPVLDDPEQGFYEVG
                     DQYFIYRSSYGSGSIINWNDIAGLTNRYLKTFPDNAAVLVTLLRSFYPSGRVSFYNMA
                     FVSDNSDTDWRLSMVFSRYWIVCLLALMAIILLIWKLAARFGRPRPLILARGRPFDEF
                     VYSMAGLFQQAKIYQMVLDNLWRDLLKAVAELTHMPVAAAPEQLIAGLNVHTGNHYRS
                     LSDFCSYFDHPTKLSKEQFLTIALQLDAYRKELTEWRKSSQFVTK"
     gene            4177..5124
                     /locus_tag="VF531_02835"
     CDS             4177..5124
                     /locus_tag="VF531_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7712933.1"
                     /translation="MEKVQSICDQVKSELTKVVIGQEKIIEGILIALFVGGHVLLEGP
                     PGVAKTLIAKSLARSITAAYKRVQFTPDLMPLDITGTNIFDLHRQQFFFKPGPIFTDL
                     LLADEINRTPPKTQAALLEAMEEKQVTVDGELKTLSPIFTVLATQNPLEYEGTYPLPE
                     AQLDRFLLKLQLSYTDSETEIEIYRRYQGNLVAENDLEQIRPVLTPDDVLEVRRMVAT
                     VNVRPEIIDYIYQIVQATRVNPLLTLGASPRAGIYLLHAAKTYAAIQGRDYVIPDDIQ
                     EVACMVLRHRLLVKPDALIDGKNSDHVLKNILASLKVPR"
     gene            5127..>6067
                     /locus_tag="VF531_02840"
     CDS             5127..>6067
                     /locus_tag="VF531_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7712934.1"
                     /translation="MFFTWYFIGFVFLLAILAVPFPGWYLGYLLACLLLLWLVDLLLT
                     MKQAKLTARRDFTDPLYQNQESEVGLVIANPNKHPVMVFIKDEPPFVVTVRNCQGGVM
                     VPAGGEAFFTYGITAPKRGIFEFGALNLKVTGRLQLFTHQTKIALRQELRVYPNLAKI
                     LNDPLLRLVGGDAAGSHRLRQLGVGGELAQLKEYTAGDDYRKINWKVTAHRGKPFINQ
                     YEPEKDQNVYLLFDTGRLLFDQVNEGASRLDHILDSAILLAFNIQEHGDMVGALSFNC
                     KVDCFVPVGKGNSHLRMLIGQLYKIQAAMVESDYRAA"
BASE COUNT         1489 a         1348 c         1574 g         1656 t
ORIGIN      
        1 acggatcgcc ttcatcggtt cggaacccca tagcgaggag atgcggaagc agatcgaaaa
       61 gctatacggg atcaaggcct ttaattccta cgggctctcc gaggtatgcg ggccgggtgt
      121 ggcctttgaa tgcccggagc aagcgggaat gcatatttgg gaggatcatt tttatattga
      181 gatcatcgat ccccggaccg ggcgggtgct gcccgagggg gaagagggcg agttggtcct
      241 aaccacgctg cggcgagagg ctatgccgct tttgcgttac cgaaccgggg atctgacccg
      301 gatcctgccc gatgcttgct cctgtggccg gacccaccgg cggattgacc gcatcaaggg
      361 ccgggccgat gacatgctca tcatcaatgg agtgaacacc ttcccgattc agatcgagca
      421 gacgatgatg cggatccccg gtgtggggaa caactattcc atcgagattc ggaaggaaaa
      481 ctatatggat aaaatgtaca ttcaggttga gtttgaccgg gatgttttcg agggaaccct
      541 tgaagagctg acgaacctcc agaaccgcgt tgccgaggaa ctcaaatcgg aactggttat
      601 ctcgccggtg gtgcggatcg tggagccggg gagtcttccg gtgtcggagg gtaaggccaa
      661 acgtgttcat gatctgagag agaaagtata agaggaggtg cataaaccat ggcgtcgcat
      721 ctgtccatct ttgtggagaa taagccggga aaactggaga aacttacccg gatcctggcg
      781 gaaaatcaaa tcaacctcaa ggcgttttca ctggccagtg tcggagagta tgggatcgtc
      841 aagatcattg tcaatgacat cgatcaagct tttgaggtgt tgaaaaagga acagattacc
      901 gtatccaaac gccggatcct catcgccacc gtgcccgatc agccgggcgg gctgcaccgg
      961 ctgctcaccg tcctttcggc caatcagatc aatattcagg attgctatgg gttcgtgatc
     1021 gaaaatcgca aggaagcggc gatcgtgatt gaagtggaga agtatcctct ggccgaggag
     1081 atcttgcgcc gcgaagagat cgggatcatg gcggacgagg acttggggaa gatctgacgg
     1141 ttcgttcttt gatttgaaag aaatttacct tcaaaatata aaacagcgaa cctatatcaa
     1201 aggtttgccg ttttttttga cttgtatata gcaatatatg gaattgatgc aggaagatta
     1261 cattcagtac cgaaaattat atttgttggg tatctttata tgtgctcttc ccaaaaagaa
     1321 caaaaggggg caggtcatga aaaatcacct aaaacctatg ggcgtacgcg atattttaga
     1381 tagtactttt acgattatgc gcgaaaattt ttggtccttg caagggattt tctttaaggc
     1441 cttttttccg tcgctgatta ttttattcgc cggaatcatc ggcgcggtgg tttatttggc
     1501 gttacttggg acgcataccg atattccaat aaccaacccg ctattctggt cggaagcagg
     1561 acacggttcg acatccgtca ttatattggg aatcgtagga ctattattgg tggcgcttgc
     1621 cttcatcgtt gccgcagtta tcggagatat ttactatact tacggagtac ttaagatatt
     1681 caaaaatgga ttgcatgatc aaaaaacctc atctaaagag gcttttcgag gagtgaaggg
     1741 taaccgggca cggatctttt gggtccagtt cgttgtggga ttattgctct atattgtgat
     1801 tttgcccggg gtcgttatat ctgtaaatgt caacctgctt tcgccgctca cgggaacctt
     1861 catttcatat gccaataatt ttttgcagtt ggtgatcgcc ttctttttct gtatgacctc
     1921 gcccgtagta gtttacgaaa atttaaaagt gatgaagtca atcgggcgcg gtatcaaatt
     1981 gttatcccat catcggtggc ggatcttctg gactctattg ctggtatatt tattgggata
     2041 tgctttgggt ctcatactgc tgggattcat cgccataccc atcgtggtgg cggcaatgct
     2101 taaaaacatg atcgcctata tcatagccgc gatctttggc atcggcgctt cgttcctgtt
     2161 ggttttattg gttgcctatt tctatgggcc attggccgcc atctattacg acctaatcat
     2221 tcgtaaagaa ggttacgata tcagtgtaca actggccgag gccgcacagg cgggaactga
     2281 aggacaatca acagcgcagc ccgcatcatt ttgaaatatc ggagtctacc attgaaaaaa
     2341 atcattttgt tatgggcact ggtcttgatg gtatttcctg cgtttgttgt ttcggccgcg
     2401 gccgccggaa ctactttgaa caatagtgat aaccgggtcc gcacggctcc acccaatatc
     2461 ccggttctac gccggaatat tcgggagata ttaaaggatc cccgttttaa ttatcccgat
     2521 cgttggaagg ggttgcagag attctggcaa aatatccgta attggttggc taagttggac
     2581 tcttcgaaaa gatccggaaa gcagatgcct tggaataagg cattacgctg gattggactt
     2641 gccgctttgg cagtattgcc tttggtctta atttatttat tacccaaact tttcgcccgt
     2701 tccgggaggg taaaatcttt atccccaaaa accgcagttc cagtggttcg ttccgaggat
     2761 ctgaaggttc aggccaggat aatggcagat aaaggtcagt ttcgcgaagc catccgcctg
     2821 ctctatctgg ccgggttgga agacctgaaa accaatggta tcctaccgga cggtgtccgc
     2881 cttaccgata aaaccaattt aaacattgta aaccgtactt ttggatctga tcatccctgc
     2941 agtcaagctt ttcgggaact gatgctggtt tttgaagaaa aatggtatgg attgcgaagc
     3001 tgtcaaacgg aggattatga ccgccttacc gggtatctga aaattttgga agccaatacg
     3061 ggtaaaccgc atgtctaaac acaaagtaat catgggtctg gtggctttag ggttactgat
     3121 gggaattttg ctcttcttaa gctgggtttc tctttcccaa aagaaactgg tggcttacga
     3181 tgccaacagt tacggcccgg acggactgaa agcgctggat cttattttga aaaaatccgg
     3241 ttatcaggta accttggttc acagtctgcc ggtcaatcat caatcactgc tgattttagt
     3301 taccactacc agactttcag ccaagcagca aaaaacaatc ctgacatggg ttagtgccgg
     3361 gggaaatgtt ttggagttag cttcatcctg gcctagcctc accagggaat cggatatatt
     3421 ggacgtgact gccggccgac cttttcaaag cgatgccgca aacctgaatt tatccggtct
     3481 gacttaccat atcagcaaaa attgggttcc tgttcttgac gatccggagc aaggttttta
     3541 cgaggtcggg gaccagtatt ttatttatcg cagctcgtac ggttcgggga gtattatcaa
     3601 ttggaatgat atcgccggtt tgaccaaccg ctatttaaag acgtttcccg ataatgcggc
     3661 ggttttagtg acactgttgc gaagttttta cccatccggg cgggtgagct tttataatat
     3721 ggcatttgta tccgataatt cggataccga ctggcggtta agtatggttt tcagtcgtta
     3781 ttggattgtt tgcctgctgg ctttgatggc gattattctg ctcatctgga aactggcggc
     3841 ccgtttcggg cgtccccggc cgttaatatt agctcggggc cgcccgtttg atgaattcgt
     3901 gtattccatg gccggacttt ttcaacaggc gaaaatctat cagatggtac tggataacct
     3961 ctggcgtgac ttgttgaagg ccgtagccga attgacccat atgccggtgg cggcagcgcc
     4021 ggaacaattg attgcgggtt tgaatgtaca taccggcaat cattaccgat ccctgtccga
     4081 cttttgttct tattttgatc atcctacgaa gttatcgaag gaacaattct tgacaattgc
     4141 tttacagctg gatgcctaca gaaaggagtt gaccgaatgg agaaagtcca gtcaatttgt
     4201 gaccaagtaa agtcggaact gacgaaggtg gtcatcggtc aggaaaagat catcgaagga
     4261 atattaatcg ctctttttgt gggagggcat gttctcctag agggtccgcc cggtgttgcc
     4321 aagacactga tagccaaatc tctggcacgt tccataacag ccgcctataa gcgggtgcag
     4381 tttaccccgg acttgatgcc ccttgatatc accggcacca atatatttga tctccatcgg
     4441 cagcagttct tcttcaaacc gggaccgata tttaccgact tgttactggc ggatgagatc
     4501 aatcggactc cgcccaaaac tcaggctgcg cttcttgaag ccatggaaga aaaacaagtc
     4561 acggttgacg gggaattaaa gacgctttcc ccgattttta cggtgctcgc cacccaaaat
     4621 cccctggaat acgagggtac gtatcccttg cccgaagccc agttggatcg ctttttacta
     4681 aaattacagt tatcctatac ggacagtgaa actgagattg agatttaccg gcgttatcag
     4741 ggtaatttgg tggccgagaa cgatttggaa cagattagac cggtacttac cccggatgat
     4801 gttttggaag tccgccggat ggtggcaacc gtcaatgtgc ggccagagat catcgattac
     4861 atttatcaaa tcgttcaggc aacccgtgtc aatcctttgc tgactttagg tgcgagtccc
     4921 cgtgcgggaa tatatttatt acatgccgcc aaaacgtatg ccgctataca gggacgtgac
     4981 tatgtgattc ccgatgatat tcaggaggtt gcctgtatgg tattgcgcca tcgtttattg
     5041 gtgaaacccg atgccttgat cgatggcaaa aatagcgatc atgtacttaa aaatattttg
     5101 gccagcttga aagtgccgag gtagccatgt tttttacctg gtattttata gggttcgtgt
     5161 tcttgctcgc gattttggcg gtgcctttcc ctggttggta tctcgggtat ctgctggcct
     5221 gcctgctgtt gttatggctg gtggatctgt tgctgaccat gaaacaagcc aagctgaccg
     5281 ccaggcgcga ttttacggat ccgctctatc aaaaccaaga gtccgaagtc ggtttggtga
     5341 tcgccaatcc caataaacat ccggtgatgg tattcataaa ggatgagccg ccatttgtag
     5401 ttaccgtgcg taattgccag ggtggagtaa tggttcccgc cggtggagag gcgtttttta
     5461 catacggcat aacggcccca aaacgcggta tctttgagtt cggcgctctc aatctaaagg
     5521 ttacaggcag gctgcaactc ttcactcatc aaaccaaaat tgcgctccgg caagagctgc
     5581 gggtttatcc aaatctggcc aaaatcttga atgacccttt attgaggctg gtcggcggtg
     5641 atgcggcggg atcccatcgc ttaaggcaac ttggcgtagg cggggagttg gcccagttaa
     5701 aggaatatac cgccggtgat gattaccgta aaatcaattg gaaagtcaca gcccatcgag
     5761 gcaagccctt tattaaccag tatgaaccgg agaaagatca aaatgtttac ctgctctttg
     5821 ataccggcag attgttgttt gatcaagtaa atgaaggcgc cagccgcctt gatcatattt
     5881 tggattcggc gatcttgctg gctttcaata ttcaggaaca tggggatatg gtgggagccc
     5941 tgagctttaa ctgcaaggtc gattgttttg tgccggttgg aaaaggcaat agccacctgc
     6001 ggatgctcat cggccagctt tacaagatcc aagcggcgat ggtggagagc gattaccgcg
     6061 cggcatt
//
LOCUS       DASVMB010000087         6664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_674269,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000087 DASVMB010000000
VERSION     DASVMB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674269"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VF531_02845"
     CDS             <1..1091
                     /locus_tag="VF531_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7712935.1"
                     /translation="QDLGIYDYQVLRRWREGANFFVELKVTFGDSKSVIPRTSDLSRH
                     ARLNWTYQTTENITTAVKTKTALALNTAQTVEVLDPETGKRIGQYKTGLQSHDVNGDR
                     YMVLEGDYVKVASLTKFNLFHLVFTWRKKFPNLSRYYLTKDACYLVDRSGLIHALNWE
                     DGSLRWNLPANSQADISEIAVERLLITLPNSELWMVDNHGVKLWAVQLGGKLLAKPIA
                     GGDEIYCPLANGDLKVLDRDSGRTISIWKVRYEGDPRNLSLGLGEKELFLAYNTQSNR
                     GYLQVFHRLTGESLWKVGWDQTVIGPPLSIADLLVIGVGNRFEARELVFGMKLWEEPT
                     YGRITRMYRQDDGVLVISGDRVYSYDVR"
     gene            1226..1489
                     /gene="ffs"
                     /locus_tag="VF531_02850"
     ncRNA           1226..1489
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF531_02850"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1641..2564
                     /gene="ispE"
                     /locus_tag="VF531_02855"
     CDS             1641..2564
                     /gene="ispE"
                     /locus_tag="VF531_02855"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7712936.1"
                     /translation="MFTIQAPAKINLALDVLAKRPDGYHEVDMIMQTITLADQLSFEL
                     MPDSEIELTTNTRLVPDDQRNLIWKAVQLIRTETGCRGGVRIRLNKRIPVAAGLAGGS
                     SDAAATLKALNFLWKLGLSLEQLLELGARLGADVPFCILQGTARCRGIGEQLTPIRRH
                     ARSTHRVLLVNPGIAVPTALIYKRLKLDQIQQHPTIDTAVAALEQDDLQNLESSWGNL
                     LEAVTCNEFPVVRRVKKYFCDFGLIHNLMSGSGPSVFALNPPETIIRPFLQGLPSGWF
                     GYLADFIDQSFDEKGDRNGTTTSSPDSVGQL"
     gene            2521..3216
                     /locus_tag="VF531_02860"
     CDS             2521..3216
                     /locus_tag="VF531_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7712937.1"
                     /translation="MERQPLVPIRLDNYKPLRELVFESLREAIIQGKLGPGERLMEIQ
                     LAEEMGVSRTPVREAIRKLELEGLVVMIPRKGAYVAGLSLKDIADVFEIRRALEGLAA
                     ELASERATDEELEQMERYLVKISEEIEVGDVDKVVETDTGFHSLLYKASRNERLTQII
                     SNLREQIQRSRTTSLSYPGRMKIAVEEHRKIVEAVSARDGELARKLAHEHIQNAENTL
                     MRMIQQDKKYGGA"
     gene            3221..3994
                     /locus_tag="VF531_02865"
     CDS             3221..3994
                     /locus_tag="VF531_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7712938.1"
                     /translation="MDAVILAGGLNSGALRRVSSAQYEAEIEIAGHPMLDYVILALKK
                     VAEIKRIVVVGPENVLSAAAKEQITVLIGPGGSVIDSLNNGLKALQTEEPILVLTSDI
                     PLITREALQDFLQRCRQRNGDIYYSFVSREVNEAKYPGVQRTYVKLREGVFTGGNLLL
                     LSPRVVRNHMSKLDKAASLRKKPIQLCRMLGWKYLFKLITGRLTIREIEERVARLLNL
                     VPVGIISPYPEVGIDVDKPSDLALACEVLTRTHGEIIRR"
     gene            4223..4477
                     /gene="spoVG"
                     /locus_tag="VF531_02870"
     CDS             4223..4477
                     /gene="spoVG"
                     /locus_tag="VF531_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010279400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX7712939.1"
                     /translation="MNITDVRLKKVNSEGKMKAIASITIDDAFVVRDIRVIEGQNGLF
                     VAMPSRKTPEGEFRDIAHPITSEARELIQSAILKEYETAQ"
     gene            4633..4707
                     /locus_tag="VF531_02875"
     tRNA            4633..4707
                     /locus_tag="VF531_02875"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4665..4667,aa:Gln,seq:ttg)
     gene            4895..6286
                     /gene="glmU"
                     /locus_tag="VF531_02880"
     CDS             4895..6286
                     /gene="glmU"
                     /locus_tag="VF531_02880"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX7712940.1"
                     /translation="MPQLASIILAAGQGTRMKSKLPKVLHPLLERPMLRYVMDAAGKA
                     GSDPILIVVGFEGQRIVEAIGPGEKYIWQHEQLGTGHAVMAAKEELSQLSGDLLVLYG
                     DTPLLREETLKELIELKRSHGACAAVLTTRMSNPAGYGRIIRDGSDQIRAIIEEKDAT
                     PEQRTIKEVNTGVYCFDIQTLLAALDQITPHNAQGEYYLTDVFKVMVGQKQKVVGLVA
                     PDAEEVMGPNDRAQLAQTERFLKLSINREWMRQGVTIEDPQYTYIGPEVAIGCDTVIL
                     PGTIILGKTKIGEGCQIGPHTRILDSTIGDGTSVQFSQVIEATLGSGNNVGPFAYLRP
                     GTSSADRVKIGDFVEVKNSVIGEGSKIPHLTYLGDTQVGANVNIGAGTITCNYDGVSK
                     HHTVIGDGAFIGSNANLVAPVTIGAGATVAAGSTITKEVPGKSLGIARSKQQNLTEWV
                     APRDRVKKQDEGE"
     gene            6422..>6664
                     /locus_tag="VF531_02885"
     CDS             6422..>6664
                     /locus_tag="VF531_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX7712941.1"
                     /translation="MELLLAATVREEVGKQKVHKLRQNGLVPAVLYGEGKPSEHITIQ
                     DHELTRLLAKNGYGKLISLEIQGGKKAEKEHVLIKDY"
BASE COUNT         1760 a         1393 c         1832 g         1679 t
ORIGIN      
        1 aacaagattt gggaatttac gactatcaag ttttacggcg ctggcgggaa ggggccaact
       61 ttttcgtgga actcaaggtg acgttcggcg attctaaatc cgtcatcccg agaaccagtg
      121 atttaagccg ccatgccaga ctgaattgga cctaccagac gacagagaat atcaccacag
      181 cagttaaaac caaaacagcc ctggctctga ataccgcaca gacagttgag gttttggatc
      241 cggagaccgg taagcggatc ggtcagtata aaaccggttt gcagtctcac gatgtcaatg
      301 gtgatcggta tatggtactt gaaggtgatt atgtcaaagt agccagtctg acgaagttca
      361 acctctttca cctggtattc acatggcgca aaaaatttcc caatctctca agatattacc
      421 taaccaaaga tgcctgctac ctggtggata gaagtggatt gattcatgcg ctaaattggg
      481 aagacggttc cttacgatgg aatttgcctg ccaacagtca ggcggatata tctgaaattg
      541 cggtagaacg actcctgatc actctaccga actcggaact atggatggtc gataaccatg
      601 gagtaaaact ctgggcagtc cagttgggtg ggaagctgct tgccaagcca atcgccggag
      661 gcgatgaaat ctattgcccc cttgccaatg gggatctaaa ggtattggat cgggattccg
      721 gccggacgat cagtatctgg aaggtcaggt atgagggtga tcctagaaac ctttcgcttg
      781 gactcggtga gaaagaactt tttttggctt acaatacgca aagcaaccgt gggtatttac
      841 aggtatttca tcgtttgacc ggagaaagcc tatggaaggt aggttgggat cagacggtta
      901 tcggtccgcc attgagtatc gctgatttac tcgtcatcgg ggtgggtaat cgttttgaag
      961 cccgtgagct ggtctttggg atgaaactgt gggaggagcc gacttatgga cggatcaccc
     1021 ggatgtatcg gcaggatgac ggggtgctgg tgatttccgg cgatcgggta tacagttatg
     1081 atgtaaggtg atggtagtat ggcctttttg gggacaaagg catactaaaa ttttcctatt
     1141 gtcaaccgtg gaaattatga tataatttta ttacttatcc attgccggat ccggataagg
     1201 atgacgaagg aaatcaagtt tatggtcgtg ctagacgggg aggtagcggt gccctgtaac
     1261 ctgcaacccg ctatagcagg gttgaattcc gggtcgcggc cgagcttcgt gaatccggct
     1321 gagttaagcg gtgttgagga ttgggtcttg cgcaacaagg acttatgaac cccgccaggt
     1381 ccggaaggaa gcagcggtaa gtgagcaact ttgtgtgccg cagggttgcc tggtcggagc
     1441 aggctgattc ggtacgttcg ggaggcttgg gtctacgctc ggtgcacggc cataatttaa
     1501 tttgtaataa ttaaccccat ggaatacagc ggttgcgata accggtgtat tttttttatg
     1561 cggcaggatt atagtcactt ttggccaata tatatatttt taagggaaaa aatactggaa
     1621 aaaaatactg gaaagaaacc atgtttacaa tccaagcccc ggctaaaata aatcttgccc
     1681 tggatgttct ggcgaagcga cccgatggtt atcatgaggt ggatatgatc atgcaaacga
     1741 tcacgcttgc ggatcaatta agctttgagc ttatgccgga ttcggaaatc gagcttacca
     1801 ccaataccag gttagtcccg gacgaccaga gaaatctgat atggaaggcg gttcaattga
     1861 tccggacgga aaccggttgc cgcggcgggg tccggattcg actcaataag cggatacctg
     1921 tagctgccgg tcttgccggt ggaagcagtg atgccgccgc taccttgaag gctttaaact
     1981 ttctttggaa actcggttta tctttggagc aattattgga actcggtgcc agattagggg
     2041 cggatgttcc tttctgcatc ttgcaaggga ctgccaggtg ccggggaatc ggcgaacaac
     2101 ttacacctat tcgaaggcat gcgaggtcta ctcatagagt gcttttggtc aacccgggaa
     2161 tcgctgttcc tactgctttg atatataaac ggctgaagct ggaccagatc caacaacacc
     2221 ctaccataga cacggctgtt gcagcgttgg aacaggatga tttgcagaac ctggagtctt
     2281 cgtgggggaa tctcctggaa gcggtcacgt gcaatgagtt cccggtggta cggagggtaa
     2341 aaaagtattt ttgcgatttc gggttaatcc ataatttgat gtccggtagc ggaccatcgg
     2401 tttttgcgtt aaatccgccg gagacaatca ttcgaccgtt tttacagggg ttgcccagcg
     2461 gctggtttgg atatcttgcg gattttatag atcaatcttt tgatgaaaag ggtgaccgta
     2521 atggaacgac aacctctagt cccgattcgg ttggacaatt ataaacccct aagggaactg
     2581 gtttttgaat ccttacgtga ggcgattatt caaggcaaac tgggtccggg tgaacggtta
     2641 atggagatcc agttggccga agagatgggg gtcagccgga ccccggttcg cgaagccatc
     2701 cggaaacttg aactggaagg gctggtagtg atgatccccc gtaagggggc ttacgttgcc
     2761 gggctctcat tgaaagacat cgccgatgtt tttgagattc ggcgagcgtt ggagggttta
     2821 gccgcggaat tggcttcgga acgagccacc gatgaagagt tggaacagat ggagcgttac
     2881 ttggtgaaga tatctgaaga gatagaagtg ggggatgtcg ataaggtcgt agagaccgat
     2941 accggttttc attccctgct ttataaggca agtcggaatg aacggctaac ccagatcatc
     3001 agtaatctgc gggaacagat tcaacgttcc cggaccactt cgttatcgta tccgggccgg
     3061 atgaagatag ctgtagagga acaccgtaaa atcgtggaag cggtatcggc ccgtgacggt
     3121 gagttagcca ggaaactcgc gcatgaacat attcaaaatg ccgaaaacac gttgatgcga
     3181 atgatccaac aggataaaaa atacgggggt gcctaactaa atggatgcag tgatcttggc
     3241 aggcggtttg aactcgggtg cgctacgccg ggttagttcg gcgcagtatg aagcggaaat
     3301 tgagatcgcc ggccatccga tgttggatta tgtaatcctg gccttaaaaa aagtggctga
     3361 gatcaaaaga atcgtggttg tcggtcctga gaatgtgtta tcggccgcgg cgaaggagca
     3421 gatcaccgta ttgattggtc ccgggggttc ggtgatcgat agtttgaaca atggtttaaa
     3481 agcactgcag acggaggaac cgattctggt gttgacttcc gatattccgc tcattacccg
     3541 cgaggccttg caagatttct tacaacgctg tcgccagaga aatggcgata tttattactc
     3601 ctttgtatca agagaggtta atgaggcaaa gtaccccggg gtccagcgta cgtacgttaa
     3661 attgcgggag ggcgtcttta ccggaggcaa tttattgcta ctctcgcccc gagtggttcg
     3721 taaccatatg tcaaagttgg ataaagccgc gtcacttcgc aaaaaaccta tccaactctg
     3781 tagaatgcta ggttggaagt atttgttcaa attgatcacc ggacggttaa ccatccgtga
     3841 gatcgaggaa cgcgtcgccc ggctcttgaa tctggtcccg gtggggatca tttcacccta
     3901 tcccgaggtg ggaatcgatg tggacaagcc aagcgatctc gcattggctt gtgaagtttt
     3961 aacccgaacc cacggggaaa tcattcgccg gtaagtcatt agtttgagcg atggaaaagg
     4021 cctcggatca aaaggccttt tttgtttggc atattcaaaa gataaaaaaa tgcagcttaa
     4081 acggtttttg taaaccaata tccaatgtca ttatatatta gaaaaattta ggaaaaaatt
     4141 ctcggaagcg gcaggagttt tttaatattt atggaaatta ttattacttc atgaagagtt
     4201 aaatagaaag gtgagtgggt ttatgaacat cacggatgta cggttaaaaa aggtcaactc
     4261 cgaggggaaa atgaaggcga ttgcttctat cacgattgat gacgcttttg tggtaaggga
     4321 tatccgggtg atcgagggtc aaaacggact attcgtggcg atgcctagcc ggaagactcc
     4381 tgaaggcgaa ttccgggata tcgctcatcc gatcacatcc gaagcccggg aactgattca
     4441 gagcgccatt cttaaagagt atgaaactgc gcaataagcg ggatttcttt ttcttacata
     4501 caaacaagtc atgatactta agtgatgaag aggctccctt aaccgggaac cttctttttt
     4561 tgatggctcg caggtacgat ttggtgattg acatcggggc aattgactga tatactattc
     4621 tttgcaatta attggggcgt agccaagtgg taaggcacgg gactttgact cccgcattcg
     4681 ttggttcgaa cccagccgcc ccagccattt agggaacaaa gaactcgact gagttctttt
     4741 ttattaagga ttgaggaaaa atttcccgct ttaagcctcc tttggctgat gattttggat
     4801 atttagctct ccaaggactt tttagggcga ctcccggtgt gcgcgtaaag cgaataccaa
     4861 acatggtcgg accctcctac aaggagtgat tggaatgccg cagttagcca gcattattct
     4921 ggctgccggg cagggtaccc ggatgaaatc caaattaccc aaagtcttac atccgttact
     4981 ggaacgccca atgctgcgat atgtgatgga cgctgccggg aaggccgggt ccgatccgat
     5041 tttgatcgtg gtgggctttg aaggccagcg gatagtggag gctatcggtc ccggggaaaa
     5101 atatatctgg caacacgaac agttgggaac cggtcatgcg gtaatggcgg ccaaagaaga
     5161 actgtcacaa ttaagtggcg atctattggt tttatatggt gatacaccgt tgttacggga
     5221 agaaacccta aaagaactga ttgaattgaa acgaagccat ggggcctgtg ccgcggtact
     5281 aaccacccgg atgagtaatc ccgccggcta tgggcggatc atccgcgacg gttcggatca
     5341 gatccgcgcc attatcgagg aaaaagacgc cactccggag cagcgaacca tcaaagaggt
     5401 caataccgga gtctattgtt ttgatataca aactcttttg gccgcgctag accagattac
     5461 tccgcataat gctcaaggag agtattattt gaccgatgtt tttaaggtca tggtgggtca
     5521 gaaacagaag gttgtaggct tggtagcccc ggatgccgaa gaggtaatgg gtccgaatga
     5581 tcgggctcaa cttgctcaaa ccgaacggtt cctgaagctt tcgatcaacc gtgagtggat
     5641 gcgtcaaggg gtaaccattg aagatccgca gtatacttat atcggacccg aggtagctat
     5701 cggttgcgat accgttatct tgcccggcac cataatttta ggaaaaacaa aaatcggcga
     5761 gggttgccag attggtccgc atacgcgaat tttggactca accattggcg atggaacttc
     5821 agtccagttc agtcaggtga ttgaggcgac gctgggatcg ggtaacaatg taggtccctt
     5881 tgcctatctt cgtcccggta cttcaagtgc cgaccgggtg aagatcggcg actttgttga
     5941 ggttaaaaat tcggtcatcg gtgaaggaag taagataccg catcttactt atctggggga
     6001 tacccaggtc ggtgcgaatg ttaatattgg agccggcaca atcacttgta actatgacgg
     6061 agtctctaag catcataccg ttattggtga tggcgctttt atcgggagta acgccaatct
     6121 ggtggctccg gtaacgattg gtgccggggc aaccgttgca gccggttcga caataaccaa
     6181 ggaagttccc ggaaaatcat tgggaatcgc ccgttccaag cagcaaaatt taaccgaatg
     6241 ggtcgctccc cgggataggg tgaagaaaca ggacgaaggc gagtaattcg ttagtttaca
     6301 accgggttca ggaatgttat aatgtgagcg atctgtgcta atttacgttt ggatctccga
     6361 cggcacttgt aaaccttggt gtctcgtctc aatatctatc taagtttagg aggagtaacg
     6421 gatggaactt ttattagcag ccacggtccg ggaggaagta ggtaaacaaa aggtacacaa
     6481 actgcgtcaa aatggtttgg tcccggcggt gttgtatggg gaaggcaaac ctagcgaaca
     6541 tatcaccatt caagatcacg aattgacccg gttattggct aagaacggtt acggtaaact
     6601 cataagcctt gagatccaag gcggtaaaaa agcagaaaaa gagcacgtcc tgatcaaaga
     6661 ttat
//
LOCUS       DASVMB010000088         8605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_10772,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000088 DASVMB010000000
VERSION     DASVMB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10772"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /gene="hisH"
                     /locus_tag="VF531_02890"
     CDS             <1..276
                     /gene="hisH"
                     /locus_tag="VF531_02890"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469093.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7712942.1"
                     /translation="ELKVHQIGWNQIHPVSETPLFRGIPDGSFVYFVHSYYVKPADPG
                     IVACTTDYGFEYCSGIAKGQTVAVQFHPEKSGKVGLIILKNFAEFKS"
     gene            273..998
                     /gene="hisA"
                     /locus_tag="VF531_02895"
     CDS             273..998
                     /gene="hisA"
                     /locus_tag="VF531_02895"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX7712943.1"
                     /translation="MIIIPAIDLLGGNCVRLHQGDPAQETFYDKDPVQVARRWEALGA
                     KRLHLVDLDGAFRGEPTQMSLVQEIIAAVSIPVEVGGGIRCLETVERYLDAGAAWVIL
                     GTAAIQSPGLVSEACRKYSGRILAGIDAKNGKVATDGWVNLTAKTALELAIELKQEGI
                     AELIYTDIGRDGTLTGPNTQALTTLAQSSGLAVIASGGISGITDLEQLRDLHLSGITG
                     AIVGKALYDKRIDLAEAIRVIEG"
     gene            1004..1780
                     /gene="hisF"
                     /locus_tag="VF531_02900"
     CDS             1004..1780
                     /gene="hisF"
                     /locus_tag="VF531_02900"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7712944.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGTEFLELRDAGDPIELAAFYDHSGA
                     DELVFLDITASHEQRKTVVEMASHVAEQVFIPFTVGGGIGSTADMRALLLAGADKTAI
                     NSAAIANPSLISEGAEKFGSQCVVVAIDARRRVKEDPFAGWEVYSHGGRIATGLDVVE
                     WAVRATKLGAGEILLTSMDRDGTADGYDIELTRAVATAVPVPVIASGGAGSLSDLADG
                     ILAGRADAVLVATLFHYRKYSIREAKEYLAGRGIVVRNEF"
     gene            complement(1850..2116)
                     /gene="rpsT"
                     /locus_tag="VF531_02905"
     CDS             complement(1850..2116)
                     /gene="rpsT"
                     /locus_tag="VF531_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7712945.1"
                     /translation="MPNIKSAKKRVDVAAVRNQANSMQTSALKTRLKKATTSLAAGEA
                     NKEVLSSAVKALDEAVTKGLIHKNQAARRKSRLTKKLNAVSAAK"
     gene            2353..3330
                     /gene="gpr"
                     /locus_tag="VF531_02910"
     CDS             2353..3330
                     /gene="gpr"
                     /locus_tag="VF531_02910"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HEX7712946.1"
                     /translation="MENNQLEEIGSIHTDLALEARELAMERGGGNDIPGMGTQTERQG
                     DTLITRVQIQTEAAGRAVGKVPGYYVTLESPGLRSRDRDQQENIAILVAKEIEGFIAR
                     LKLKDEDHCLVVGLGNWDATPDALGPRVISHILVTRHLTESTPPEKRGELRPVAALAP
                     GVLGTTGLETGEIVLGVVQRIKPQFLVVVDALASRSTTRMGATIQIADTGIHPGSGLG
                     NKRIGITPQTLGIPVIAIGVPTVVEATTIIHDALEEIYRGAPALVNPRTINHQDLVGR
                     VLSPYLHSLIVTPKEINVMIEDLARVVSGALNIALHPGVSPGEVFRYLS"
     gene            3450..4220
                     /locus_tag="VF531_02915"
     CDS             3450..4220
                     /locus_tag="VF531_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712947.1"
                     /translation="MKLRYRSRFRALLPFYAYSGILSFLLGLSGVLLSNGTAAILVHR
                     TVRQFEAWNGNLRNYWQQETGQWIQSPVVPADTTAVPEDDTTIPQLIVGYPEKPGIAG
                     FDSWFLSMGRRLDQKFPGWISIPFKPSNWPEQSIYGLSQEYDRGIFIGFNIREVNDPE
                     KPAIQTDLYLYLPDKGMSLYGSKIAAAVLARIGDGSLRKQDRGIHVLYEISWAKRIEI
                     IRNHPLEANSEYWSGLTSTLDALLEQFEPDRRTGIRFD"
     gene            4259..4489
                     /locus_tag="VF531_02920"
     CDS             4259..4489
                     /locus_tag="VF531_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712948.1"
                     /translation="MKRKVFFFIWLWAFLLLGAGYYLTLNNLGFLLNESGPGGGFPGV
                     KWIWQKGELIINCGSWHEAVTLDFWPSIGRKQ"
     gene            4880..6475
                     /gene="murJ"
                     /locus_tag="VF531_02925"
     CDS             4880..6475
                     /gene="murJ"
                     /locus_tag="VF531_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7712949.1"
                     /translation="MDTKTSERAAKAAGMISLLYLLSRILGFVRESLSGQLFNRFQTD
                     AYIAAFTIPDAMYYLLVGGALSAAFIPIFTEYLAKGKEQEGWEVASTFMNITVLLLAC
                     FTLLGVIFAEGVAPLEAPNFAIVPAKMQLLVELTRIMFPAVCFTAMAGMVGGALNSYQ
                     HFLAPALGPVFYNIAIIGAAVLLGPKWGIKGMAIGVVFGAFGNFLIQAFFLSRFSGRK
                     YRFGYIDLKNPGFRRMILLMIPALIGLSATQVNIWITNVMASYLPEGSITAIRFANRL
                     IQLPIGIFAAGIATAFFPMLSGMAAKEEMDSFKDSLSLALRSIFFLMIPSAVGLILLR
                     KPIVALLFQGHHFTVHDTELTSYALLFYSLAIFAHAAILMLPRAFYALKDTKTPVIVS
                     VSSVLLSIMLNYIFLRFTKLGVGGFALSFSMMGLVNMLSLMAILRRKIGGIRGGRILR
                     SFLKCLGASLAMGAALWYLNPVFLSLIQSLHLTGRMADGFIVAGAMAVGVAVFFGSAF
                     LLKMEELHMALRMIKRRLHRKAA"
     gene            6560..7276
                     /locus_tag="VF531_02930"
     CDS             6560..7276
                     /locus_tag="VF531_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX7712950.1"
                     /translation="MSNLAVIFAQAFVVGLSGALMPGPLLTYNIQLSYKKGFWTGPQL
                     VLGHAILEAALILGLLGGLGSFIQLKATRIALGILGGLMLFWMGTDLIRTESKKGPAA
                     NVESAASGETGTVINLPPILAGIVISATNPYFLLWWAVLGLAMITQAYTLGWLGVAAF
                     YFGHIFSDLSWYSLISAVIVKGKRFISEQIYRWLLLICGLFLIALALWFVWDALNLLG
                     LIHWIFERSRNLFEPIRRFV"
     gene            7286..>8605
                     /gene="lepA"
                     /locus_tag="VF531_02935"
     CDS             7286..>8605
                     /gene="lepA"
                     /locus_tag="VF531_02935"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7712951.1"
                     /translation="MGLIQTHIRNFCIIAHIDHGKSTLADRLLEYTGALSKREMTDQV
                     LDSMDLERERGITIKEKAVRLEYQSQDGEKYILNLIDTPGHVDFTYEVSRALAACDGA
                     LLIIDATQGVEAQTIANLYLALEHNLDIIPVINKIDLPSAEPDRVKQEIRDIIGLDPD
                     EAILASAKTGEGTPEILEAIVHRIKPPRGEERLPLRALIFDSLFESYRGVIVYLRVVE
                     GRLAVGQKIRMMITGKEFEITEIGTFRPMMVPTTELTTGEVGYLAASIKNVKDIEVGD
                     TITDVENFALDPLPGYRTVQPMVYCGLYPVDNAAYGDLRDALDKLRLNDASLVYEPEN
                     SAALGFGFRCGFLGLLHMEIIQERLEREYDLNLIATAPSVVYKVYLTDGTVKTISNPA
                     EMPVANFLEHLEEPYVKATIILPSDFVGPIMDLCQEKRGVFKNMEYLT"
BASE COUNT         1987 a         1980 c         2378 g         2260 t
ORIGIN      
        1 gaactgaagg tgcaccagat aggatggaat cagattcatc ctgtatcaga aactccttta
       61 tttcggggta tcccggatgg gagttttgtt tatttcgttc actcctatta cgtcaaaccg
      121 gcggatccgg gaattgttgc ctgtactacg gattacggtt tcgaatattg ctcggggatc
      181 gccaagggtc aaacggtagc ggttcagttt caccccgaaa aaagcggcaa ggtaggactg
      241 atcattttaa aaaattttgc ggagttcaaa tcatgattat tataccggct atcgatctac
      301 tgggcggcaa ctgcgttcgg cttcatcagg gggatcctgc ccaagaaact ttttatgaca
      361 aagatccggt ccaagttgcc cgtaggtggg aagcactggg ggccaaacga ttacatctag
      421 tcgatctgga tggcgctttc cggggtgaac cgacacagat gtcgttggtt caagagatta
      481 ttgcggcagt ctccatccct gtcgaagtcg gtggcggtat ccgatgtcta gagacggtag
      541 aacgttatct ggatgccggt gctgcatggg taatattggg gacagctgca attcaaagcc
      601 cgggactggt gagtgaggcc tgccggaaat attccgggcg gattctggcc ggaatcgacg
      661 ctaaaaacgg taaggtggcg acggatggtt gggttaacct gacggccaag accgccttgg
      721 aactggcaat cgagttaaaa caagaaggaa ttgcggagct catctatacc gatatcggcc
      781 gggatggaac gctcacgggt cctaatactc aggccctaac caccctggcc caatcaagcg
      841 gtcttgccgt aattgcctcg gggggaatct cagggattac agatttggag caattacggg
      901 atttgcatct gtccgggatc accggagcga ttgtcggcaa ggcgctttac gataaacgga
      961 ttgatctggc tgaggccatc cgagtgattg aggggtagtt attatgttgg caaaaaggat
     1021 cattccatgc ctggatgtaa aagacggccg ggtagtcaag ggaaccgaat ttttggaatt
     1081 acgggatgcc ggagatccaa tcgaattagc tgctttttac gaccattccg gagccgacga
     1141 gttggttttc ctggacatca ccgcttcgca cgaacagcgt aagacggtgg tggagatggc
     1201 atcccatgtc gcggaacagg tttttatccc atttacggtg ggtggcggaa ttggtagtac
     1261 cgccgatatg cgggctttac tgctggccgg agccgataaa accgcgatta attccgcggc
     1321 gattgcaaat ccctcgctaa tcagcgaggg agctgagaaa tttggctccc agtgtgtggt
     1381 tgtggctatc gacgcccgcc gtcgcgtgaa agaggatcct tttgccgggt gggaagtata
     1441 tagccatggt ggccggatcg ctacaggact tgatgttgtg gaatgggctg ttcgtgccac
     1501 caaacttggg gcaggggaga ttctcttgac cagtatggat cgggacggga ccgcggacgg
     1561 atatgatatc gaattgaccc gtgccgtcgc aacggcggtt cccgttccgg ttatcgcttc
     1621 gggcggcgcc gggagtcttt cggatttggc agatggaatt ctagccggcc gggcggacgc
     1681 ggttttagta gcaacgcttt tccattaccg gaagtattcc ataagagagg ctaaggaata
     1741 tctggccggg agagggatcg tagttcgaaa cgaattttga gaaaaacaaa agggactgac
     1801 ttttaaagtc ggtcccttta tcgatcgttc cttcgcaaac attatcaaat tatttcgcag
     1861 ctgatacggc gttgagcttt ttggtaagcc ttgatttacg ccgggctgct tgatttttat
     1921 ggatcaagcc cttggtaacg gcttcatcta aagccttgac ggcactggat aaaacttctt
     1981 tattggcctc acctgcagca agggaggtag tcgctttttt aaggcgcgtt ttcaacgcgg
     2041 aagtctgcat tgaattggct tgattacgta cagcggcaac gtcaacccgt tttttggcgg
     2101 atttaatatt cggcaaaaca ttcacctcct taaactgtag gcatgattcg accagaaccg
     2161 atcaaaccta attccccaaa ttttagacag taattatttt atcatgggat caaccgattt
     2221 gcaaggaaaa accatttttt tatcctctct ccctaaattt gagagaccgc accggaaatt
     2281 atcgggctga tacttaaaaa cacctcaacc gggaagacta aggaggtcaa gaaaattttt
     2341 accggaggaa atatggaaaa taatcaactg gaggagatcg gctccattca taccgattta
     2401 gcccttgaag ccagagaact ggcaatggaa aggggcgggg gcaatgacat ccccggtatg
     2461 ggaacccaga ccgaacggca gggcgacacg ttaatcacgc gggtgcaaat acaaaccgag
     2521 gccgccggtc gggcggtggg aaaggtaccg ggctattatg taaccttgga atctcccgga
     2581 ttacggagtc gcgaccggga tcagcaggaa aacatcgcca tattggttgc caaggagatc
     2641 gagggtttca tcgcccggtt gaagttaaag gatgaggatc attgtctggt ggtaggcttg
     2701 ggaaattggg atgccactcc ggatgcacta ggcccccggg tcatctcaca tatcttggta
     2761 acccggcatc tgacggagtc aactcctccc gaaaaaaggg gcgagttgcg tccggtggcg
     2821 gccctggccc ctggagtatt gggaacgaca ggacttgaga cgggggagat cgttctgggg
     2881 gtagtccaac ggatcaaacc ccagttcttg gtagttgtcg acgctttggc atcacgcagt
     2941 accactcgga tgggggcaac gattcaaatc gcagataccg gaattcatcc cggttcggga
     3001 ctggggaaca aacggatcgg gattactccc caaactctgg gaattccggt gattgccatc
     3061 ggggtgccta ctgtggttga ggcgactacc atcatccacg atgccctgga agagatttac
     3121 cgcggcgccc cggcattggt taatcccagg acaataaacc atcaagactt ggtgggtagg
     3181 gtcctatctc cctatcttca cagtttaatt gtgaccccga aggagattaa cgtgatgatt
     3241 gaggatttgg ccagggttgt ctccggggcg ttgaatattg cactgcatcc cggggtcagt
     3301 cccggggaag tattccgtta tctttcgtaa cgtttgacct atgataattg atccagagcg
     3361 ttatgtgatg ggtatcgtag aattcgcaac cggggagcgg ttctaaaact gagtcccgtt
     3421 tcatatcttc ggttcgtgga gtgatacgaa tgaagctacg atacaggagc cggtttcgag
     3481 ctctgctccc tttctacgct tactcaggaa tccttagttt tttattggga ttgagcggag
     3541 tattattgag caatggcaca gcggccattc tcgttcatcg gactgtccgt cagtttgaag
     3601 catggaatgg caatctccgc aactactggc aacaggagac gggtcagtgg attcaatcac
     3661 cggtagttcc ggccgatact accgcggtac cggaggatga tacaacgatt ccgcagttaa
     3721 tcgtcggtta tcccgaaaaa ccgggaatcg ccggttttga ttcctggttt ttgagtatgg
     3781 ggcgccgact ggatcagaaa ttcccgggtt ggatttctat tccgttcaag ccatcgaatt
     3841 ggccggaaca aagtatatac gggctatctc aagaatatga ccggggaatt ttcattggat
     3901 tcaatatccg ggaagtgaat gatccggaaa aaccggcaat ccagaccgac ttataccttt
     3961 acctacccga taaagggatg agcctatacg gttcgaaaat agctgccgca gtacttgccc
     4021 ggattggtga tggttccttg cgcaaacagg atcggggcat tcatgtgtta tatgagattt
     4081 catgggcaaa aaggatcgaa atcatccgga atcacccgct ggaggcgaat tcagagtatt
     4141 ggtccgggct aacatcgacc ttggatgcgt tgctggaaca gtttgagccg gaccggcgaa
     4201 ccggaattcg tttcgattga ggggctaatc cggtaattca ttggttacga ggtggaatat
     4261 gaagcgcaag gtctttttct ttatttggct ctgggcattc ttgttattgg gagccggtta
     4321 ttatttgacc ctgaataatc tgggatttct tctgaatgaa tccggtcccg gaggggggtt
     4381 ccccggagtc aaatggatct ggcagaaggg ggagcttatc atcaattgcg gttcctggca
     4441 cgaggcagtg acccttgatt tctggccaag catcgggcga aaacagtaaa atgaggcaac
     4501 aggcaacagg gaacaggcaa taggggaata ggcaagtcgt tcgatgaagt cgaactcatc
     4561 cttctccaat tgccttttta tgcataaccc attgcttatt gtttatagtc tattaacaat
     4621 tcacctccta cgcgcctctt ttttcagaaa gagtgcgcgt tttttaaaca cgagtgtatg
     4681 gtcatagtga aagtatatga ttaacgtggg gcgctactta cgtctcaagc ctcaagcctc
     4741 gtgcctcaag cctcgtgcct caagccttaa tctttctgtt gcctattgcc tgttccctgt
     4801 tgcccaggat actttacatt ccttgaagga tatcgctccg atatggtgaa tactaaatgc
     4861 ctggaaaggg gatctcgata tggacacgaa aacatcggag cgggcggcca aagcagccgg
     4921 gatgatctcg cttttgtatt tgctcagccg gattttggga tttgtacggg aaagtctctc
     4981 cgggcagtta ttcaaccgtt ttcaaaccga cgcgtatata gcggcgttta ctatcccgga
     5041 tgccatgtat tacctgctgg tagggggagc cctgtcagcg gcttttattc caatcttcac
     5101 cgaatatttg gccaagggga aagagcaaga gggctgggaa gtcgccagca ctttcatgaa
     5161 tatcaccgta ttgctgcttg cctgtttcac gttactgggg gtgatctttg ccgagggagt
     5221 ggctccgctt gaggcaccca atttcgcgat cgtaccggcc aagatgcaac tgttggtgga
     5281 gttgacccgt atcatgttcc cggctgtttg tttcaccgca atggccggaa tggttggcgg
     5341 cgctctcaac tcttatcagc actttctggc tccggccctc ggaccggttt tttataatat
     5401 cgccatcatc ggtgcagccg ttctcttagg cccgaaatgg ggaattaaag gcatggccat
     5461 cggtgtggtg ttcggagctt ttggaaactt cctgattcag gcgttttttt taagtcggtt
     5521 tagcggcagg aaataccgtt tcgggtatat cgatttgaag aatcccggtt tccggcggat
     5581 gattttactg atgatacctg ctcttattgg cctctctgct acgcaggtga atatctggat
     5641 caccaatgtc atggcatcct atcttccgga ggggagcatt accgcgattc gttttgccaa
     5701 tcgtttgatt cagctaccca ttggaatctt cgccgccgga atcgccaccg cattttttcc
     5761 catgttatcg ggaatggcgg ctaaggaaga aatggactcc ttcaaggatt ccctgtccct
     5821 ggcgttgcgc tcgatcttct ttttgatgat cccgtcggct gtgggattga tcctcctgag
     5881 aaaacctatc gtggctttac tcttccaggg acatcatttt accgtccatg atacggagct
     5941 tacctcctat gcgcttctct tttatagctt ggcgatcttc gcccacgccg caattttgat
     6001 gctgccccgg gctttttatg ccctcaagga tacaaagacc ccggttattg taagcgtctc
     6061 ctcggtattg ctcagcatta tgctaaacta tatcttcttg cgttttacca agctgggggt
     6121 gggtggtttc gccctttcct tctcaatgat gggcctggtg aacatgctct cgttgatggc
     6181 aattctaagg cggaagatcg gcggaatccg tggcggtcga attttacgat cgtttctgaa
     6241 atgtcttggc gcgtcgctgg ccatgggagc tgcactctgg tacctaaatc cggtcttttt
     6301 atctctgatt caaagtctgc acctgacggg acgaatggcg gacgggttta tcgtggccgg
     6361 ggcgatggct gtcggggtgg cggtcttttt cgggagtgcg tttctcttaa aaatggaaga
     6421 gcttcatatg gccctgagaa tgattaagcg ccgcttgcat cgtaaagccg catgatgagg
     6481 cgagtgtttc cggtttgtga ctataggcgg tgacgtttgc cggaataact gtattacctg
     6541 ctgggagagg ttgatggcaa tgagtaattt agcggttatt ttcgcccagg catttgtggt
     6601 tgggctttcc ggcgccctga tgccggggcc gttattgact tacaacattc aactttcata
     6661 taaaaaaggt ttttggaccg ggccccaatt ggtcctggga catgcaatcc tggaggccgc
     6721 cttaatcctg ggactcctcg gcggtctggg gagcttcatt cagctcaaag cgacccggat
     6781 cgccctgggt attttggggg gattgatgct attctggatg ggtacagatt tgatccggac
     6841 cgagagtaaa aaaggtccgg cggcaaatgt cgaatctgcg gcttctgggg agacgggtac
     6901 ggtgatcaat ctccctccaa ttttggcagg gatcgtaatc tccgcgacca atccttattt
     6961 ccttttatgg tgggccgtat tgggactggc tatgattacc caggcatata ctttggggtg
     7021 gttaggagtg gcggccttct atttcggcca tattttctcg gacctcagtt ggtacagttt
     7081 aatttccgct gtgatcgtca agggaaaacg attcatctcg gagcaaatat accgttggtt
     7141 attgctcatc tgcggcctgt ttctgatcgc tctggcactc tggtttgtct gggatgcctt
     7201 gaaccttctc gggctcatcc attggatttt cgagcgaagc cgcaatctct ttgaacctat
     7261 tcgcagattt gtatagtttg gaggaatcgg tttgattcag acccatatca gaaatttttg
     7321 catcatcgcc catatcgatc atgggaaatc gaccctcgcg gatcgattgc tcgaatatac
     7381 cggggcctta tctaaacggg aaatgaccga tcaggtcttg gactcgatgg atctggaacg
     7441 ggaacggggc ataaccatca aggaaaaggc agtacgtttg gaatatcaaa gccaggatgg
     7501 ggagaagtat atcctgaacc tgatcgatac cccggggcac gtggatttta cgtatgaggt
     7561 atcccgggct ctggcggcct gtgatggagc actgttgatc atcgatgcaa ctcagggggt
     7621 tgaggcacaa accattgcca acctttacct tgccctggaa cacaatctgg acatcattcc
     7681 ggtgatcaac aagatcgact tgcccagcgc ggagccggac cgggtgaaac aggagatcag
     7741 agatatcatt gggcttgatc cggatgaggc gatcttggca agcgccaaaa ccggagaagg
     7801 aaccccagag atcctggaag cgattgttca ccggatcaaa ccgccccgtg gcgaggaacg
     7861 tttaccgtta cgcgccttga tcttcgattc gctctttgaa tcctaccggg gagtcatcgt
     7921 ctatctccgg gttgtcgaag gccggcttgc ggtaggccaa aagattcgga tgatgataac
     7981 gggtaaggag tttgaaataa ctgaaatcgg tactttccgg ccgatgatgg ttccaaccac
     8041 cgaactcact acaggtgagg tcggttatct ggctgcctcg atcaagaatg tgaaagatat
     8101 cgaggtgggc gataccatca ccgatgtaga aaacttcgca cttgatccgt tgccgggtta
     8161 ccggacggta caaccgatgg tctattgcgg gttgtatccg gtggataacg cggcctacgg
     8221 cgacttgcgc gatgccttgg acaaattgag actgaatgat gcttcgttag tttacgaacc
     8281 ggaaaactcc gcggccttgg gtttcggttt ccgttgcggt tttttgggat tattgcatat
     8341 ggagatcatc caggaacgtt tggaaaggga gtacgaccta aatctgatcg ccaccgcgcc
     8401 cagtgtggtc tacaaggtgt accttaccga cgggacggtg aaaaccatct ctaatccggc
     8461 tgaaatgccg gttgccaatt tcttggagca cctggaggaa ccctatgtca aagccaccat
     8521 cattctgccc agtgattttg tagggccgat catggatctc tgccaggaaa aacggggtgt
     8581 ttttaagaac atggaatatc tcacc
//
LOCUS       DASVMB010000089         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_157961,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000089 DASVMB010000000
VERSION     DASVMB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157961"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VF531_02940"
     CDS             complement(<1..240)
                     /locus_tag="VF531_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX7712952.1"
                     /translation="MRKKKAEEPENSERWMLTYLDMITLLMAFFCILFAMANVNATKF
                     KQVAQSMSMAFGTGGGGGSSMLTNFSGTGISPQTAT"
     gene            complement(246..1046)
                     /locus_tag="VF531_02945"
     CDS             complement(246..1046)
                     /locus_tag="VF531_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEX7712953.1"
                     /translation="MDIATLLGIIIAFVSVFGGSALEGKFEGLPALLNPPAALIVFGG
                     TIGATMVCFRMSAIASIGKYFGAAFKQEKLDITELINEMVRYAEMARKEGLLTLEEMI
                     ANVKNQFLKKGIQLIVDGTDPTLVREILEIETDYMEERHKMGINMFKQAGGFAPTMGI
                     IGTVLGLIGVLANMSDTASLGPSISTAFVATFYGVFSANVLWLPLSQKLAVSTQEEIS
                     ARQIIIEGILSIQAGEHPRVISEKLTSFLPPANRPAPVKSGEGFGARG"
     gene            complement(1229..1846)
                     /locus_tag="VF531_02950"
     CDS             complement(1229..1846)
                     /locus_tag="VF531_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7712954.1"
                     /translation="MKQLRNSFFTGLLVLLPIVVTLVLFWWGFKKADSILGGFFTRYL
                     GYPIPGLGLIALLVLITVFGIIAQNYLGKKLIAAGERLLNKIPVLKAVYGTTKQFTSN
                     FTQNERSAFRQMVLVEYPRPGIYSPGFLIGDAPAEVGVKTKNRLLSIFMPTVPNPTTG
                     YLILVPDDQVTFLDMSVEEGIKLIISAGVVLPEERSGRGNLSDPK"
     gene            complement(1918..>3525)
                     /gene="pepF"
                     /locus_tag="VF531_02955"
     CDS             complement(1918..>3525)
                     /gene="pepF"
                     /locus_tag="VF531_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX7712955.1"
                     /translation="GRVLDKLFIFARMHKDENNADSVYQALTDRVQALGTEASTALSF
                     IVPALVDFPHEKRQSYMEENSDLRIYRHFFEEIDRQRDHVLPGPEEKLLAETMDLADA
                     AGNVFGMLDNADIKFPYITDENGESVELTKGRFSRFMESKDRRVRQEAFAALYSTYTK
                     FRNTFGATLNGSVKSDYFYARVRHFNSALEASLESDNVPVNVYGRLIEVIHEYLPVLQ
                     DYLKLRKRMLGLGELHMYDLYTPLIPEYQRSFDYEEAKRLVLEGLKPLSDEYLAQVRQ
                     ALEDRWIDVYENEGKTSGAYSWGAYDSHPYMLMNYQGKIHDVFTLAHEMGHSMHSYYS
                     NHHQPYVYAGYKIFVAEVASTVNEALMMEYLLAHTQDQQEKQYLLNQYLEQFRTTIFR
                     QTMFAEFEKLIHDQVEQGGALTADWFSETYYNLNQLYYGAETVVDQEIAMEWSRIPHF
                     YSGFYVYKYATGYAAAVALSQQILREGVTARERYLQFLRSGDSDYPLNLLRNAGVDMA
                     EPQPVREALNVFKNLISQLSDLAGVRV"
BASE COUNT          896 a          933 c          828 g          868 t
ORIGIN      
        1 agtcgcagtc tgcggagaga tcccggtgcc gctgaaattc gtcagcatac tggatccgcc
       61 gccgcccccg gtaccgaagg ccatactcat cgactgggcg acttgcttaa acttagtcgc
      121 attcacattg gccatggcga acaagataca aaaaaatgcc atgagaagcg taatcatgtc
      181 cagataagtt aacatccagc gttcggaatt ttcgggttct tctgctttct tttttctcat
      241 aatttttaac ctctagcgcc gaagccttcg ccggatttaa ccggagcggg ccggttcgca
      301 ggcggcagga acgatgtaag tttctcactg attacccgtg gatgctcgcc ggcctgaatc
      361 gagagaatgc cctcgataat gatttgccgg gcggagatct cttcctgggt actgactgcc
      421 aacttttggg acaggggcag ccacaacacg ttggcggaga aaaccccata aaaagtggct
      481 acgaaagcgg tagagatgga gggtcccaga cttgcggtat cggacatatt ggccaaaacg
      541 ccgataagtc cgagcaccgt accgatgata cccatcgtcg gggcgaaacc tccggcctgt
      601 ttgaacatgt taattcccat cttatgccgt tcctccatgt aatcggtctc gatctccaaa
      661 atctcccgga ccagtgtggg gtcggtgccg tcgacgatta actgaatgcc cttctttaaa
      721 aactggttct tgacattggc aatcatctct tccaaagtaa gtaatccttc cttacgggcc
      781 atctcggcgt accggaccat ttcattaatc aattccgtga tatccagttt ctcctgtttg
      841 aaagcggctc cgaaatattt tccgatactt gcaattgccg acatccgaaa gcacaccatc
      901 gtggcgccga tggtgccgcc gaaaacgatt aaagcggccg gaggatttaa caaagccggc
      961 agaccttcga acttcccttc gagcgcgctg ccgccaaaga ctgaaacgaa cgctatgata
     1021 attcccagta atgttgcgat atccatcttt gtttcactcc aatatttaat atcatttatt
     1081 ttatcggtcc atcccggcga taagtttagt catttccgcg gtttaaaaca aaaacccggg
     1141 tcttactggg taatcccggg taatcattca tatcactaaa attgtgacac ttcttggagt
     1201 agacgattta cttagtatca accccttttt atttgggatc ggataaattc ccgcgtccgc
     1261 ttcgctcctc gggcaggacc accccggctg agataatgag cttgatccct tcctcaacac
     1321 tcatgtctaa gaaggttacc tgatcatcgg gaaccaggat caagtacccg gtagtcggat
     1381 tgggaacagt gggcataaag atactaagca gtcggttttt tgttttaacg ccgacttcag
     1441 ctggagcatc cccgatcaga aaaccggggc tgtaaatccc gggacgggga tactccacca
     1501 gcaccatctg ccggaaagcg ctgcgttcgt tttgggtaaa attgctggta aactgtttcg
     1561 tagtaccgta aaccgctttc aaaacgggta tcttgtttag caagcgctcc ccggcggcaa
     1621 tcagtttctt ccccagataa ttctgggcaa ttatgccaaa aacagtgatc aataccagga
     1681 gtgcaatgag gcccaatccg ggaatgggat agcccaagta ccgggtgaag aagcccccca
     1741 agattgagtc ggccttttta aacccccacc agaacaatac caaagtcacc actatcggta
     1801 gcagaaccaa tagtccagtg aaaaaggagt tccgcagctg cttcaacaac cgtctccacc
     1861 accatgcgac agattaagat ccgcggtagc agccctttat tctttgaccg ttctaactca
     1921 gaccctgact ccggccagat cgctcagttg agagatgagg tttttaaaga catttagcgc
     1981 ctcgcggacg ggttggggtt cggccatatc cactccggca tttcgtaata gatttaaagg
     2041 ataatcggaa tctccgctcc gtaggaactg cagataccgt tctctggccg tgactccttc
     2101 tcttaaaatc tgctgggaaa gagcaactgc agccgcgtat ccggtagcgt atttatatac
     2161 gtaaaaaccg gagtaaaaat gagggatccg ggaccactcc atcgcaatct cctggtcaac
     2221 cacagtctcg gccccataat aaagctggtt caggttgtaa tatgtctcgg agaaccaatc
     2281 ggccgtcaac gctccaccct gctccacctg atcgtggatt agcttttcaa actctgcaaa
     2341 catggtctgc cggaagattg tggtccggaa ctgctccaga tactggttga gcagatattg
     2401 tttttcctgc tgatcctggg tatgggccaa caaatactcc atcatcaggg cctcgttgac
     2461 tgtggaagcc acttcagcca caaagatctt ataccccgcg tacacatagg gttgatgatg
     2521 gttggagtaa taactgtgca tcgaatggcc catctcatgc gccagggtaa agacatcgtg
     2581 gatcttaccc tggtagttca ttaacatata agggtggctg tcgtaagcgc cccaggaata
     2641 agccccgctt gtcttgccct cattctcgta gacgtcaatc cacctgtctt ccagcgcctg
     2701 acggacttgg gccaaatatt catcgctcaa gggtttcaaa ccttcgagca cgagccgttt
     2761 ggcctcctca taatcaaaac tgcgttggta ctccgggatg agcggagtat acaggtcata
     2821 catatgcaac tcgccaaggc cgagcatccg tttgcggagt tttaaataat cctgaagaac
     2881 cggcaggtat tcatggatta cctcgatcaa ccggccgtaa acatttaccg gtacgttatc
     2941 gctttccagg gaagcctcaa gcgccgaatt gaaatgacgg actcgggcgt aaaaataatc
     3001 agatttcacc gaaccgttca aagtagcccc aaaagtgttg cgaaatttcg tatatgtact
     3061 gtatagcgcg gcgaaagcct cctgcctgac ccgccggtcc ttgctctcca taaaacggga
     3121 gaagcggcct ttggtcaatt cgacgctttc gccgttctca tcggtaatat agggaaactt
     3181 gatatcggca ttatccagca taccaaagac atttccggcg gcatcggcca gatccatggt
     3241 ttcggcgagt aatttctcct ccggacccgg tagcacatgg tcgcgttgac gatcaatctc
     3301 ctcaaagaaa tgccggtaaa ttcggagatc ggaattctct tccatatagg attggcgttt
     3361 ctcgtgagga aaatcaacca gggcgggaac gataaaagag agcgccgtgc tcgcctcggt
     3421 ccccagggcc tgaacccgat cggtcaaagc ctggtagacc gaatccgcgt tattttcatc
     3481 cttatgcatc cgggcaaaga taaaaagttt atccaacacc cgtcc
//
LOCUS       DASVMB010000090         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_158061,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000090 DASVMB010000000
VERSION     DASVMB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158061"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1184
                     /locus_tag="VF531_02960"
     CDS             225..1184
                     /locus_tag="VF531_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX7712956.1"
                     /translation="MRSLKIHWLAFILIGLTMSSGCPGGEGTYFRNMVTRFGKPPQIS
                     VYMKDTGEKRRMDLEEYLVGVVAGEMKPGWPLNAYAAQAIIARTFTMEFLGRGGTRAL
                     HGTDISTDEKEAQAYNAAAITASVRRAVQMTKGWVLTYKDRYIKGWYSASCGGLTTYA
                     KEGLAYKEPEPAYIQSVKCPEAKVIPNSELYWQTTLTSDAVNNALRKLNQKPVGTVNK
                     MQIVKRTSTERATLLRFSGDQGAAEVAGADFRVNYGPDKIRSIWISNQIANHPGAITI
                     KGRGFGHGVGLCQWGSYALAKMGRSPRAIVTHYYPKAHMSKIW"
     gene            1389..1676
                     /gene="yabP"
                     /locus_tag="VF531_02965"
     CDS             1389..1676
                     /gene="yabP"
                     /locus_tag="VF531_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HEX7712957.1"
                     /translation="MDEKKKNNEIPHQLTLANRGLLSIEGVLDLGSYDQEQIILDTNC
                     GALEIKGERLHVQQLNLDQGKVLVDGDIHSLVYTAEMAGKKGKGFFSKLMK"
     gene            1978..2817
                     /locus_tag="VF531_02970"
     CDS             1978..2817
                     /locus_tag="VF531_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7712958.1"
                     /translation="MPSRTGTKRKSQPLPIFRWVSLLCKFLISPVVMLIDSFRRKGVL
                     DQIWLYSFFLFTTVVMALTGVGILVTEEPLYRTLRMMATVTESDEVLSGDLQPLIRVI
                     NKYALEYNVDPNLVFAIIRTESNFRRTAVSRAGAKGLMQIMPQVWQEYSGSACDGRHS
                     NKIICHPDDCIFDADSNIRVGVKYFRALLDKYQGRADLALEAYNAGISNVRPGFDPKF
                     LETKNYVSGIISFWNQLRKETIAQQLKISLQMQRGLKWLFIAAFSCWLILFWWAGRKW
                     FGK"
     gene            2909..>3088
                     /gene="yabQ"
                     /locus_tag="VF531_02975"
     CDS             2909..>3088
                     /gene="yabQ"
                     /locus_tag="VF531_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HEX7712959.1"
                     /translation="MEYVEQQLSSCWVLLMTGMVLGLFFDFYRVFRGRAGFKSGRGLF
                     GALADLVFWCLALILI"
BASE COUNT          761 a          681 c          870 g          776 t
ORIGIN      
        1 tttggatatc aatcctgagt tggccttgcg ccgaaccatt gacaaattca accaacgctt
       61 ccgttatatc gagtccgagg cagctaaggc cggccgggag ctcaccgaga tgactctggc
      121 cgaaatggat gaactctggg agaaggccaa gtaatgtagc gggcggtata aaaagaactc
      181 aagatgagat gctagcgaat gaaacgatga tggagggtga tgggatgcgc tccttaaaga
      241 ttcattggct ggcattcatt ttaatcgggc ttacgatgag ctcgggctgt cctggcggtg
      301 aagggaccta tttcagaaat atggtgaccc ggtttgggaa accccctcag atctcggttt
      361 acatgaaaga taccggtgag aagcgtcgga tggacctgga ggaatatttg gtgggggtcg
      421 tcgcagggga gatgaaaccg ggttggccgc tcaatgcata cgcggcccag gccattatcg
      481 cccggacctt tactatggaa ttcctgggta ggggcggcac ccgggccttg catgggaccg
      541 atatatcgac cgatgagaag gaagctcaag cttataatgc cgcggcgatc actgcttcgg
      601 ttcgccgggc tgtacagatg accaagggat gggtattgac ctataaggat cgttatatta
      661 agggatggta ctcggccagt tgcggcgggt tgacaacata cgccaaggag ggtttggcct
      721 ataaagaacc tgaaccggct tacattcagt cggtcaaatg tccggaggca aaagtcattc
      781 caaattccga actatactgg cagaccacat tgaccagcga cgcagtgaac aatgcgctgc
      841 ggaagttgaa tcagaaaccg gtcggcaccg ttaataagat gcagatcgtc aagcgtacct
      901 ccactgaacg ggcgacatta ctccgattta gcggcgacca gggggccgcc gaggtggccg
      961 gggcggattt ccgcgttaat tacggtccgg ataagatccg ttccatctgg atctcaaacc
     1021 agatcgcgaa tcatccgggg gcgattacga ttaagggccg gggcttcggc catggagtgg
     1081 ggttatgcca atgggggtcc tacgccctgg caaaaatggg ccgttcaccc cgggccattg
     1141 taacccatta ttatcccaag gcccatatga gtaagatttg gtaaaaagtg agcgaaactt
     1201 aaaaagccgg ccaacgagcg ccggcttttt ccatctgaat ctccaatcag atcgcaagtc
     1261 atcgggagcg attcggagcc atctttcccc gcggcttcta agttgtcttt ttaccgaata
     1321 tcttatattt gccggataat tgataagtgg aggtctgatt ttcgatgttg aaccgggggg
     1381 aactaacgat ggacgagaag aaaaaaaaca atgaaatccc gcatcagttg acgctggcca
     1441 accgtggtct gttgagcatc gaaggcgtgc tggatctggg gagctacgat caagaacaga
     1501 tcattttgga caccaactgc ggagctcttg agatcaaagg ggagcgtctg catgtccagc
     1561 aattgaatct ggaccagggg aaagttttgg tggacgggga tattcattcc ctggtttata
     1621 cagccgaaat ggcgggaaag aaaggaaagg gattcttcag caaactgatg aaatgaatca
     1681 gagatcaact tggaaatatc gggaaggatt cttccaagcg gcgccgaatt attataatag
     1741 tgcagatctt cgttccattt ttggacggtt atgattcacc cctgacgcgc gtaactacaa
     1801 cttataccgg ttactcaaaa tgggattgcc ttgagtgagt ggtataatgc cagaaacttc
     1861 gttttgtggc atgagaatct tctccggggt ctgtgcggca agcccaagtg gccaagcccg
     1921 gcagttgaat gatgactatc tgaaatggaa tttattattt tcaaaggagt tttcaagatg
     1981 ccttcccgga cgggtaccaa acgcaaatct caaccgctcc cgatttttcg ctgggtgagt
     2041 ttgttatgca aatttttaat cagcccggtt gtgatgctga tcgattcctt tcggcgaaaa
     2101 ggcgtgttgg atcagatttg gctatattct ttctttttat tcaccacggt ggtcatggcc
     2161 ctgaccggag taggaatcct agttaccgag gagccgctct atagaacctt acggatgatg
     2221 gcgacagtaa ccgagagtga cgaggtgtta tccggggatt tgcagccgtt gatcagggtc
     2281 atcaataagt atgctttgga atacaatgtc gacccaaatt tggtctttgc gatcatccga
     2341 accgaaagta attttcggcg taccgcggtc tcacgggctg gcgccaaggg cttgatgcag
     2401 atcatgcctc aagtttggca ggaatatagc ggttcggctt gcgacggccg gcacagtaac
     2461 aaaattatct gtcatcccga tgattgtatc ttcgatgcgg attccaacat ccgggtaggg
     2521 gtcaagtatt tccgtgccct gctcgataag tatcagggcc gggctgacct ggcgctcgag
     2581 gcgtataacg ccggaatttc caatgtccgg ccgggttttg atcctaaatt tttagagacg
     2641 aaaaactatg tctccgggat catttcgttc tggaatcagc tccgcaagga gaccatcgcc
     2701 caacaattga agatatccct gcagatgcaa cggggtttaa agtggctgtt tatcgcggct
     2761 ttcagctgtt ggctgatact tttctggtgg gccgggcgta aatggtttgg taaataagtc
     2821 aaccggcctg cacctggtga gcgttatgaa tccgcccttc ccggcataaa gtactttata
     2881 ggttaatccg ataggggaag agcgcggaat ggaatatgtg gaacaacagt taagttcatg
     2941 ttgggtgctc cttatgaccg gaatggtctt ggggctcttt ttcgattttt acagggtctt
     3001 tcgcggacgg gcggggttca aatccggccg gggtctcttt ggggccctgg ctgatttagt
     3061 attttggtgt ttagcattga ttttgatt
//
LOCUS       DASVMB010000091         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_527694,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000091 DASVMB010000000
VERSION     DASVMB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527694"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="VF531_02980"
     CDS             complement(<1..2260)
                     /locus_tag="VF531_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7712960.1"
                     /translation="MNQNPEQKARDQIDQMLAASGWSVQNKQSINFNVASGGVAVREY
                     LTDVGPVDYVLFVDRNPVGVIEAKKEEEGHNLTLVEDQSVEYATSKLKHLNNNPLPFV
                     YESTGTLTRFTDYRDPKPRSRVVFSFHHPETFRDWFKQNKTLRARLHDIPELKTEGLR
                     ECQIRAIRNLEHSFKENRPRALIQMATGSGKTFTAITFIYRLLKYADAKRILFLVDTK
                     NLGEQAEQEFMAYLPNDDNRKFTELYNVQRLKSSYISTDNQVYISTIQRLYSILKEEE
                     LDESAEEENPAESAWQAKEPMPVVYNAQLPIEFFDFIVIDECHRSIYNLWKQVLDYFD
                     AFLIGLTATPDKRTFAFFNENVVSEYTHEQAVADGVNVGHDVYIIETEITRKGSAIWG
                     GEYVDKRERLTRRTRWEQLDEDLIYNGKQLDRDVVNPSQIRNIIRTFKERLPEIFPGR
                     NEVPKTLIFAKTDSHADDIIQMVREEFGEGNNFCKKITYRIDDDPKTVLSQFRNDYYP
                     RIAVTVDMIATGTDVKPLECLIFMRDVKSRNYFEQMKGRGTRTIKKDDLLKVTPSAKA
                     KTHFVIVDAVGVTKSLKTDSRPLEKKPSVPLKDLLAAVTFGAQEEELYTSLANRLARL
                     EKQITDSERQLFIEKAGGKTINQVVSELFQAYNPDAIEDQAQFIRAKNPDLPHETALK
                     QAQQELIDMARSTFNGELNIYIDNVRRAHEQIIDTVNLDTVTGVGWDNDVADKAQAII
                     QDFREYLEAHKDE"
     gene            complement(2590..>2889)
                     /locus_tag="VF531_02985"
     CDS             complement(2590..>2889)
                     /locus_tag="VF531_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712961.1"
                     /translation="EMFISPEEYITKGIELLGARKIGEGFLKLLNNPKYKEIQSVIAV
                     GEMAKGSIDPKLVAYLQYILDKWHQGDETVRGWLMIQLGIAFQEVAKKLQEEKKE"
BASE COUNT          755 a          719 c          558 g          857 t
ORIGIN      
        1 tctcatcttt atgggcttca agatattccc ggaaatcttg gataatggct tgcgccttat
       61 cggcaacatc gttatcccaa cccactcccg ttacagtatc gagattcacc gtatcgatga
      121 tttgctcatg tgcgcggcgg acattatcga tataaatgtt taactctcca ttgaaagtgg
      181 atcgagccat atcaattagc tcttgttgcg cctgttttaa agccgtttca tgaggaagat
      241 ccggattctt agcccgaatg aattgagcct gatcttcaat ggcatccggg ttataggcct
      301 ggaaaagttc gctgaccact tgattaattg tcttgccgcc agccttctcg atgaaaagtt
      361 gccgttcact atcggtgatc tgtttctcca aacgggccaa acgattggcc agcgaagtgt
      421 acagctcttc ctcctgcgcg ccaaaggtga cggccgccag taaatctttt aacggcacgg
      481 atggtttctt ctccagcggc ctgctgtcgg tctttaagga cttggtgacg ccaactgcat
      541 cgacaatgac gaaatgggtt ttggcctttg ccgatggggt tacttttagc agatcatcct
      601 ttttgatggt gcgggtgccg cgccctttca tctgctcgaa gtaattccgg ctcttgacgt
      661 cccgcataaa gatcaaacat tccagcggtt tcacatccgt gccggtggca atcatatcta
      721 ccgtaaccgc aatgcgcgga taataatcgt tacgaaactg ggacaaaacc gtcttggggt
      781 catcatcaat ccgataggta atcttcttgc aaaaattatt cccctcgcca aactcttctc
      841 gcaccatttg aataatatca tcggcatggc tgtcagtctt agcaaagatc aatgttttgg
      901 gaacttcatt cctgcccgga aagatctctg gcagccgttc cttgaaagta cggataatat
      961 tgcggatctg gcttggattg accacatccc gatccaattg ctttccatta taaatcaagt
     1021 cttcatccag ctgctcccat ctggtcctcc gggtaagccg ttcccgcttg tccacatact
     1081 ccccgcccca aatcgcgctg cctttccggg tgatctcggt ttcaataata tagacgtcat
     1141 ggccgacatt cacgccgtcg gcgactgcct gttcgtgagt atattcactg actacatttt
     1201 cattaaagaa ggcgaaagtc cgtttatccg gggtagcggt caatccaatc aggaaagcat
     1261 caaaataatc caatacctgc ttccataaat tataaatgga acgatggcat tcatcaatca
     1321 caataaaatc aaagaactca atcggaagtt gagcattata aacaaccggc atgggttctt
     1381 tggcctgcca ggcgctctcc gccggattct cttcctcggc ggattcatcc aattcctctt
     1441 ctttgagaat cgaatacagc cgttgaatgg tggagatata gacctgatta tcggtggaaa
     1501 tataagacga tttcaaacgt tggacgttat acaactcggt aaatttacgg ttatcatcat
     1561 tgggaagata agccataaac tcttgttccg cttgctcgcc gagatttttg gtatcgacta
     1621 aaaataaaat ccttttggca tcggcatact tcaataggcg ataaataaaa gtaatcgccg
     1681 tgaaagtctt cccggacccg gtcgccattt gaattaaagc gcgcggccga ttttccttga
     1741 aagagtgctc taaattgcga atagcccgaa tctggcactc ccgcaaacct tcggttttta
     1801 attcgggaat atcatgtaat cgagcccgga gtgtcttatt ttgcttaaac cagtcgcgaa
     1861 atgtttccgg gtgatgaaat gagaagacaa ctctagagcg gggtttgggg tcccgataat
     1921 cggtaaagcg ggttaaagtg ccggtgcttt catatacaaa aggcaaagga ttattattga
     1981 gatgctttaa tttgctggta gcatattcaa cggattgatc ttcaactaac gtcagattat
     2041 ggccttcttc ttcttttttg gcttcgataa ctccaacagg atttcgatcc acaaaaagca
     2101 cataatcaac cgggccaaca tcggtaagat attcgcgaac cgcaacacca cccgatgcga
     2161 cattaaaatt gatcgattgt ttgttttgga cagaccagcc ggatgctgca agcatctgat
     2221 cgatttggtc acgtgccttt tgttcgggat tttgattcat tgcatgttcc tttagcaata
     2281 aattaaaaca gtaacttatt ttttagattt caataaataa tcccagtttc ctccaaaata
     2341 ttcttagacc gagcaattat caaaccttat acccccctta aggaattaga tatactagac
     2401 aatgctctgt atattccgca tatttcattg aattcgtcgt cacctcaaac ctcttgatct
     2461 tcctatcctt ctccaaaagc tccttcctcc tgattctcaa caactcattc accgccccga
     2521 tctccctcac tgtcatctca aaatattttc tcaacaaaat aggttcttcg aaaatcgaat
     2581 gaacctagat tattccttct tctcttcttg taacttcttc gccacttcct gaaacgctat
     2641 ccccaattgt atcatcagcc aaccccggac tgtctcatcc ccctgatgcc acttatccaa
     2701 gatatattgt aaatatgcta ctagtttcgg gtcaatactc ccctttgcca tctctccaac
     2761 cgctattacc gattgtattt ctttgtattt gggattattt aaaagcttta aaaaaccttc
     2821 cccaatcttt cgcgctccca ataattcaat ccccttggta atatactctt caggagaaat
     2881 aaacatttc
//
LOCUS       DASVMB010000092        14587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_599611,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000092 DASVMB010000000
VERSION     DASVMB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14587
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599611"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VF531_02990"
     CDS             complement(<1..350)
                     /locus_tag="VF531_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712962.1"
                     /translation="MNIKEIKELVKMLDGTDINELSIESEGNKVVIKKGTNNLPYMNP
                     QFSPMVQFPMTATPVITQGGTAPAAANPAASVQAGEEEKNEGLGPNQVLIIAPMVGTF
                     YRSPSPDAEPYVQVG"
     gene            complement(536..1057)
                     /locus_tag="VF531_02995"
     CDS             complement(536..1057)
                     /locus_tag="VF531_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HEX7712963.1"
                     /translation="MMERFLETLRHDKLWLGVGCLLFLGLFWFGYRQIIRVNQPAQTP
                     QIQEQAPPKIPREYNPSDSLLDLKIERDRERSQQVEQVRELLEKVGLSDDIRKQAEQE
                     LWRLTQATVKEQELETLLEAKGFKDVLVTISRGLVTIVINGKLDPELVRQIGQVTMDV
                     TAFDLDQVEIVEH"
     gene            complement(1060..1674)
                     /locus_tag="VF531_03000"
     CDS             complement(1060..1674)
                     /locus_tag="VF531_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712964.1"
                     /translation="MNETGKGIQSNLKELWGSLRTGMKSQKPASFLFLLILAGVGVIL
                     VVLGNGSAPFTTRQMAPTSTSVPVTGGAAESVESRMEQDLERTLEGIAGVGAVTVDLD
                     LKSKNRMVWERQTQTSKRVTQEQAGVNTEESVEDQLVFAKGSDGLDSPVLKEELAPEI
                     LGVVVVAEGAEDSRVKQLLTETIKTVLGIPAHRVLVVPGEHGGS"
     gene            complement(1667..2212)
                     /locus_tag="VF531_03005"
     CDS             complement(1667..2212)
                     /locus_tag="VF531_03005"
                     /inference="COORDINATES: protein motif:HMM:NF021121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HEX7712965.1"
                     /translation="MQDLADWLKRIIAAVILAGFLELILPQNNLKGVTKMVMGLVIIT
                     LLFQPFTHLLRLPSEIRWDLPGLDAVDSNPASSRTAAVVENGFEMREKWAARLTQDSR
                     AIVETKVKTLVSLIDEVRLKQLQMEYSGSNLVKVHLLVSPAVPGGLSETLLKTITKRL
                     NESVRLVSDLPADRIEVIWNE"
     gene            complement(2258..3442)
                     /gene="spoIIIAE"
                     /locus_tag="VF531_03010"
     CDS             complement(2258..3442)
                     /gene="spoIIIAE"
                     /locus_tag="VF531_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HEX7712966.1"
                     /translation="MEKWHRILFIEVITLGMMILDPYLAVAVDSGKSGMTSTDSAVRA
                     AAAELDLREMQQFLNTLDSETQKLLPRFAPQEWGLVGPTWDFRKIARGVLDYFLRELV
                     FNLRLLGELLLLAMALAILENLRHAFESDTVSQLSFALCFLVVMGLVLNSLRVTMNLA
                     AETMTQMTNFVYAIIPLMFSLIVAGGGVTTAAIVHPMLISSVGVMAVFVNNIVFPLIV
                     FAGVLGMASFLVEGFQLGKLANLMKNLALGLLGLGMAIFIGIITVRGFAASVADSTAI
                     RTAKYFSNTFLPVVGKPFSDTMEMAAGCSLVLKSGLGVYGLGLIVLITAFPLLKILAV
                     AIVYQLTGAVAQPLGNPRLADALNHAGGIFINIFAAVAVVGLMFFITLAVLVGLANYG
                     MG"
     gene            complement(3543..3926)
                     /gene="spoIIIAD"
                     /locus_tag="VF531_03015"
     CDS             complement(3543..3926)
                     /gene="spoIIIAD"
                     /locus_tag="VF531_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HEX7712967.1"
                     /translation="MTFMAILGFSLVVTVLLVILRKERPEIAVLLGMAATGLILAGLL
                     GNIGRVLAVFETLAIKSQMNLEYLKLIVKIVGLAYLAGFGAQICKDAGENGMAAKIEL
                     AGKIFILTMGIPIMVGLLDLILQLF"
     gene            complement(3937..4131)
                     /gene="spoIIIAC"
                     /locus_tag="VF531_03020"
     CDS             complement(3937..4131)
                     /gene="spoIIIAC"
                     /locus_tag="VF531_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HEX7712968.1"
                     /translation="MDINLIFRIAGIGILISVLNMVLKQAGKEEQAQMLTLAGVVIVL
                     LMVIQLIQRLFNDVRSVFGL"
     gene            complement(4414..4932)
                     /locus_tag="VF531_03025"
     CDS             complement(4414..4932)
                     /locus_tag="VF531_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HEX7712969.1"
                     /translation="MIKTIGAALILGAGTVAGFSMARRLNERCRLLQLWLRIIEIFKA
                     EIYYQSRLLPEVFRRAAVLCDDSMMADGLRKLADSIGYGTSFEIDVVWRDFLHKSSGD
                     MLATGDITILTDLGNYLGGTDREDQGSKLEFCKLALKHNLQLAEAERDRKTGVFRYSG
                     FAVGAVLVLLLL"
     gene            complement(5024..6061)
                     /gene="spoIIIAA"
                     /locus_tag="VF531_03030"
     CDS             complement(5024..6061)
                     /gene="spoIIIAA"
                     /locus_tag="VF531_03030"
                     /inference="COORDINATES: protein motif:HMM:NF040318.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HEX7712970.1"
                     /translation="MNDPEYKNGAMGTESISSFAAAKVLPHWAGVLNFFPPSLRQLLQ
                     AIPPDLQQQVIEIRLRINQSLELNLGQQSNWVTPQGELRSDLASGYRVSPGDIKQLLN
                     AVTTGSFYAVEEELLGGYLALPGGHRIGVTGQAVVNCGKIRLIKNISSINFRIARSIR
                     GIARNILPVLWKDGHLLKTLIIAPPAAGKTTLLREIIREISNGVECLQIPPLHVGLVD
                     ERSEIAGSYLGVAQLDVGPCTDILDGCSKQEGVYLLLRAMNPQVIATDEVGRETDIPV
                     IEEIINAGVSFIATAHARNLTEAMLRPGLKRILETGSIERLIFLSSRTGVGTIESVKA
                     GLAGPELFKGR"
     gene            complement(6202..6639)
                     /locus_tag="VF531_03035"
     CDS             complement(6202..6639)
                     /locus_tag="VF531_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7712971.1"
                     /translation="MSDLKAKAAYLRGLIEGADFPKNEKEKLVWEGLMDFCDGIAADF
                     GEMDESQNEFAEYIEAIDEDLSSLEKYFYNTSDSDEEDTDVVFSEDNGKPVMEMACPH
                     CNEEIYFEDEPGNYEVVCPECGKVVWSRFMAENTPPEKTEDLS"
     gene            complement(7058..7621)
                     /gene="efp"
                     /locus_tag="VF531_03040"
     CDS             complement(7058..7621)
                     /gene="efp"
                     /locus_tag="VF531_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7712972.1"
                     /translation="MISVTDFKTGMTIEIDGVLYTVIEFMHVKPGKGAAFVRSKLKNI
                     KTGFTVERTFNSNEKVEAAHIDFREMQYLYKSDDDYTFMDTENYEQLTIPASKMGDAP
                     KYLKENMLVEIQIYQNDILGVDLPNTVELEVTETDPTFKGDTATGGSKPATLETGVVV
                     NVPFFVVTGDKIQVDTRTNQYLKRITK"
     gene            complement(7738..8808)
                     /locus_tag="VF531_03045"
     CDS             complement(7738..8808)
                     /locus_tag="VF531_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7712973.1"
                     /translation="MDRLTKLREAMQKMDLDGMLITAPQNQRYLSGFSGGEGALLIGA
                     DTAVIVTDFRYWEQVAQEVKAFTLHKQTEKLLPTIARLLTEMKWRRIGFESAQVTFGE
                     YQLLRELIPAGQELIPAGEMVAEIRWIKDAEEIELLAQAEAITDRAWQATMQLIKAGV
                     QERELALEFDYQIRKNGAEGNSFTTIVASGFRGALPHASASSKTIAPGELVTLDGGAL
                     YHGYCADLTRTVVLGKADPKQHEVYQTVLEAQERALKVLRAGMIGSKVDAVARDYIAG
                     RGYGANFGHGLGHSVGLQIHEEPRLSLTETHEIPVGAVITVEPGIYLPGWGGVRIEDL
                     VVVETDGIRNLTNSPKQNLIEL"
     gene            complement(8828..9286)
                     /gene="aroQ"
                     /locus_tag="VF531_03050"
     CDS             complement(8828..9286)
                     /gene="aroQ"
                     /locus_tag="VF531_03050"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX7712974.1"
                     /translation="MHHILVLHGPNLNWQGKREPGVYGSQTLEDINRLIQLEAAEMDC
                     EVKIFQQNSEGGLIDSIQAEAEWAEGILINPGAYTHYSYALRDALVSVELPVVEVHLS
                     NIYSREEFRRHSVIAPIAVGQIAGFGYNSYLLGLRALQDLLSGKTVGNKH"
     gene            complement(9287..10378)
                     /gene="aroB"
                     /locus_tag="VF531_03055"
     CDS             complement(9287..10378)
                     /gene="aroB"
                     /locus_tag="VF531_03055"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7712975.1"
                     /translation="MEEVRVNLGERSYPIYIDQGLIARTGRLIREQGLDGKVLVVTNP
                     TVAEWYQEPLLHSLTESGFSVEFAMVPDGETTKSLEEANRLYDLLVAGGYDRHSLLLA
                     LGGGVIGDLTGFVAATYMRGVRFIQIPTTLLAQVDSSIGGKVAVNHPRGKNLIGSFYQ
                     PGMVVADVAVLKTLPHRELASGMAEVIKHGIILDGNYFRFLSGELPQIGRVEPQIMTW
                     VVSGSCRIKGAIVAGDERESGSRALLNFGHTIGHALESVTHYTRFKHGEAISLGMLAA
                     VDIAKALGILEEPELGNSLREVFAELGLPTTLVDLPSTPICEAIRMDKKAISGQVRWI
                     LPRRLGEAVISGDVPEKLVLQVLQAMGGS"
     gene            complement(10421..11599)
                     /gene="aroC"
                     /locus_tag="VF531_03060"
     CDS             complement(10421..11599)
                     /gene="aroC"
                     /locus_tag="VF531_03060"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7712976.1"
                     /translation="MMGNTFGRVFRITTCGESYAGGFRKQLDIPKELYGGLMTIVDGV
                     PPGVKITAELVQKELDKRRPGRSALDSPRKETDRVYIFSGVMEDDLSTGAPVGLVIPN
                     VDIEDVHIEQYRSYQDVVRPGHAEYTYFKKYGEYADWVGAGRASGRETASRVAGGAIA
                     QAILDRIGIDVIGFVTESHGIKAGPISYEIAKANYRKNDLNCPDLEMAEKMIADILAV
                     KEAGDTCGGLVEVIAHGVPAGLGEPVFDKLTATIAHGIMSIGAIKGLEFGAGFEHARM
                     TGSQSNDLPYFDESSGRVRFTTNHSGGFLGGITNGEEIRIRMAVKPTPTVSVPQTTVD
                     MKQMRNVTLQPITRRDPSLCPRIYPVCEAMVRIAILDALYMAHGYQAVAGLDPKWDRV
                     "
     gene            complement(11692..12969)
                     /gene="aroA"
                     /locus_tag="VF531_03065"
     CDS             complement(11692..12969)
                     /gene="aroA"
                     /locus_tag="VF531_03065"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7712977.1"
                     /translation="MLLKVSNSQAGGRVRIPGSKSQTIRALFLASLAQGRSEIHAPLI
                     SSDTLSAVTTCQAFGARIIQEEQKFTIEGINGLPQVPQDIIQVGNSGTTLRFGVMTAA
                     LCDGYTVFTGDEQIRSRPLGPLLTAICDLGGIAFTTRDNGSAPAVIKGRVHSGAISVD
                     AVTSQYLSALLIHAPLLDGDTTIRVTRLNEAPYVDMTLGWLDGQRISYDRDGYRQFTV
                     KGGQHYQAFRTTIPGDFSSATFLMVQAAISGSEMILENLDLKDTQGDKAILPVLESMG
                     ANVTVTGDTIRIVGNGLKGREIDLNAIPDALPALAVAGCFASGETRLVNVPQARLKET
                     DRIRVMAAELVKMGADITELPDGLVIKSSSLKGCFLNGHSDHRVVMALAIAGLNASGI
                     TLIDSAEAVRVTFPEFVDIISACGGKIRLMAEG"
     gene            13202..14548
                     /locus_tag="VF531_03070"
     CDS             13202..14548
                     /locus_tag="VF531_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7712978.1"
                     /translation="MTHVFRQYDIRGVVDSDLSIDFVADLGKAIAAFFKQRKVETVSL
                     GFDCRLSSIPFHDRLVESFVTSGLTVYDIGMVPTPIVYFSLFNLPVDGGVMITGSHNP
                     PNENGFKICLGKSTIFGEDIQEIGRIMDERRFISGQGRLIERSLIPDYLSYVQKIIHP
                     GPKPLKVVVDAGNGTGNLTSVPLYRSLGWEVIELYSEPDGNFPHHHPDPTVPAYLVDL
                     KAKVAEIGADLGIALDGDADRIGVIDAKGRTLWGDQLLQIYAAEILQAKPGAKIIGEV
                     KCSQKLFDRIRELGGVPIMWKVGHSLLKAKMKEEQAVLAGEMSGHLFFADRYYGYDDA
                     VYSGARLLEILSHSTKSLVEMVDALPKTYATPEIRFECPDDRKFQVVRDLTEHFRKEY
                     QVIDIDGARIIFPDGWGLVRASNTQPVLVLRFEADSQTALTRIEAEVRAELDVLVK"
BASE COUNT         3645 a         4070 c         3274 g         3598 t
ORIGIN      
        1 ccgacttgca catatggttc tgcgtcggga ctcggcgaac gatagaaggt ccccaccatc
       61 ggcgcaataa ttaagacttg gtttggccca agtccctcat ttttttcctc ctcacccgct
      121 tgaactgaag ctgccggatt ggctgcagcc ggggctgttc ctccctgagt gataactgga
      181 gtagcggtca ttgggaactg aaccatggga ctgaactggg ggttcatata tggaagatta
      241 ttcgttcctt ttttgattac tactttattt ccttcactct cgatacttaa ttcgttgatg
      301 tcggtaccat ccaacatctt aaccagttcc ttgatctctt tgatattcat tcaacgtcct
      361 ccttttgggg gaatgggaaa agatgataga ctatttattc accagtaagg gtatttttcc
      421 ttcttttaat taattttatc ttttgcatgc gtaaatatac gaccggactc gcgccgtttt
      481 aaacattgcg gtgcgagtca ttcgatgatt gaggctgagc cttcgccacg cgttattagt
      541 gctcgacgat ctctacctgg tccagatcaa aagcggtgac atccatggta acctgaccaa
      601 tctgcctaac cagctccgga tcaagctttc cattgatgac gatggtaacc aagccccggc
      661 taatggtaac caacacatct ttgaaccctt tcgcctcaag taatgtctca agttcctgct
      721 ctttgactgt agcctgcgtt aaccgccaaa gttcctgctc agcctgtttc cggatatcat
      781 cggaaaggcc tactttctct agcagctcac ggacttgttc cacttgttgg ctccgttccc
      841 gatcgcgttc tatttttaaa tcaagcaatg aatcagaagg gttatattcc cggggtatct
      901 taggcggcgc ctgttcctga atctgaggtg tttgggccgg ttgattgacg cggatgatct
      961 gccggtaacc gaaccaaaaa aggccgagaa ataatagaca accgacccct aaccagagtt
     1021 tatcatggcg caatgtttcc aagaatcgct ccatcattat cagctccccc cgtgttctcc
     1081 gggcaccacc aatacccgat gggccggtat acccaaaacc gtcttgatcg tttcagtgag
     1141 caactgtttt acccggctgt cctcggcacc ttcggccaca acaaccacac ccaaaatctc
     1201 cggagccagc tcttctttaa gaaccggact atcgagccca tcactgcctt tcgcgaaaac
     1261 caattgatcc tccacgctct cctcggtatt gactccggcc tgttcctggg taacccgttt
     1321 actggtctga gtttgccgct cccaaaccat ccggtttttc gattttagat caagatccac
     1381 tgtcactgcg ccgactcctg caattccctc caaggtccgt tccagatcct gttccattcg
     1441 cgattcgaca gattctgccg ctccgccggt gaccggaacc gatgtactcg taggtgccat
     1501 ctgccgggtt gtaaatgggg cagacccatt tcccaacacg actaaaatca ctccgacccc
     1561 ggctagaatc aacaagaaca gaaatgaggc gggtttttga gacttcatgc cggtgcggag
     1621 acttccccat agctccttta ggttcgactg gataccctta ccggtttcat tcattccaaa
     1681 tcacctcaat ccggtcagcc ggtaaatcgc tgaccaatcg gaccgattcg ttcaaccttt
     1741 tggtaatcgt tttcagcagc gtttccgaaa gcccccccgg caccgccgga gacactaata
     1801 aatgcacttt caccagatta gaaccggagt attccatctg tagctgtttc agccggactt
     1861 catcgatcag ggatactaag gttttaacct ttgtttccac gatcgctctg gagtcttggg
     1921 ttaaccgcgc agcccacttt tcccgcatct caaatccgtt ttcaaccact gcggcagtcc
     1981 tactgctggc cggattggaa tcaactgcgt caagccccgg taaatcccag cgaatctcgg
     2041 aaggaagcct tagcaaatgg gtgaaaggct gaaacaaaag cgtgatgatc accagcccca
     2101 ttaccatttt ggtgacgccc tttaaattgt tttgcggtaa gatcagctcc aaaaagccgg
     2161 ctaatattac agccgcgata atacgtttca accaatccgc aagatcctgc atgatcgtcc
     2221 ctcgtagacc cccacccatg cgtggggata ccaataatta tcccatacca taattggcca
     2281 agccaaccaa tacagccaag gtgatgaaga acatcagccc gaccactgcc acagcagcaa
     2341 aaatattgat gaagataccg cccgcatgat tcaaagcatc ggctagcctc gggtttccca
     2401 gaggttgagc taccgccccc gttaattgat aaacgatggc cacagccagg atcttcaaca
     2461 acggaaaagc ggtgatcaaa acgattagcc ccaacccata tacacccaga ccgcttttga
     2521 gcaccagcga gcatccggcc gccatctcca tggtatcgga gaagggcttg cctactaccg
     2581 ggagaaaggt attggagaag tactttgccg ttcgaatcgc ggtactgtct gccaccgagg
     2641 ctgcgaagcc gcgaacggtg ataattccga tgaatatcgc catccccaaa ccaagtaggc
     2701 ctaaagccag gttcttcatc agattggcca atttccccaa ttggaaacct tccactagaa
     2761 aactggccat tcccagaact ccggcaaata cgattaacgg aaagacgatg ttgtttacga
     2821 aaaccgccat caccccaacg ctagaaatga gcatcggatg aacgattgcc gctgtggtca
     2881 cacccccgcc ggccacaatc agactaaaca tcagcggaat gatagcatat acgaaattcg
     2941 tcatctgggt catcgtttcc gcggcaaggt tcattgttac ccggaggcta tttagtacca
     3001 atcccatcac cactaagaaa cagagggcaa aactcaactg gctgacagta tcactttcaa
     3061 aggcatggcg gagattttcc aatatggcca gggccattgc caataataac agttcgccca
     3121 ataagcgaag gttaaaaacc aattcccgca ggaaataatc aagcaccccc cgggcaattt
     3181 tcctaaaatc ccatgttggc ccaaccaatc cccattcctg aggggcaaaa cggggtaaca
     3241 gcttctgagt ctcagaatcc agtgtgttaa gaaattgttg catttctcgg agatccaatt
     3301 ccgctgcggc agctctaacc gcactatcgg tcgatgtcat tcccgacttc cctgaatcga
     3361 cggctaccgc cagatacgga tcgagtatca tcattcccaa tgttatgact tcgatgaata
     3421 aaatcctgtg ccatttttcc atggtaaact tgactcccgg gcaaccactt ttttaatgga
     3481 atgatatttc gatcattagg tccggcattc cagctgtata cgggcatccg gttggattaa
     3541 actcaaaaga gctgcaaaat cagatcgagt agcccgacca taatcggaat ccccatcgtc
     3601 agaatgaaaa tcttccccgc cagttcaatc ttggctgcca tcccgttttc cccggcatct
     3661 ttgcaaatct gggcaccgaa tccagccagg taagcgaggc caacgatttt aacgatcagc
     3721 tttagatact ccagattcat ttgggacttg atggccaaag tctcaaagac cgctaataca
     3781 cggccgatat taccgagaag cccggcaaga atcaatccgg ttgcggccat ccccaataat
     3841 acggcaattt ccgggcgttc cttgcggaga atgactagca gcaccgtcac caccagcgaa
     3901 aaaccgagaa tggccatgaa tgtcatgggg gtcctcctat aaaccaaaga ccgatcggac
     3961 atcattgaac aatctttgaa tgagttgaat aaccatcaaa aggacaatta ccaccccggc
     4021 cagagtcaac atctgggctt gttcttcctt tccagcctgt ttgagtacca tattcagtac
     4081 ggagattaaa atgccgattc ccgcaatgcg gaagatcaaa ttgatgtcca tcttccgata
     4141 ctcctcatgt cgaaagtcta ttaaggcaac aggcaatagg caaaaggcaa aagtattgta
     4201 agtcatttat tatagtattg aagtcaaaag ttcgaaatcg tccagcagta actcaccccc
     4261 gtcccctctt ttcaaaaaga gaggtcgcgt taaaaacgcg gcgtaaggtt aactgaacgt
     4321 acataggtac cgcgggttga gttgcctcat gcctcgtgcc tcacccctgt tgcctgttgc
     4381 ctgttcccta ttgcctgttc tcatgcctct tgcctacaaa agtaacaaca ccagcaccgc
     4441 tcccacggca aatcccgaat accgaaatac tccggtcttt cgatcccgtt ccgcctcagc
     4501 cagttgcaga ttatgtttca aagccagttt gcaaaattca agcttcgagc cctgatcttc
     4561 ccgatcggtt cctcccaggt aatttcccaa atcggtcaag attgtgatat ctcctgttgc
     4621 caacatatca ccactacttt tgtggaggaa atcacgccag accacatcta tttcaaaaga
     4681 tgttccatat ccgatagagt ccgccaattt ccggagtccg tcggccatca tcgagtcatc
     4741 acaaagaacc gcagcgcgcc ggaaaacctc cggtagtagc cgggactggt aataaatctc
     4801 cgctttaaaa atctcgatga tacgcaacca tagttggagc aggcggcagc gttcgttcaa
     4861 acgccgtgcc atgctaaaac ccgccaccgt gccggcaccg agtatcagtg cagcgccaat
     4921 cgtcttaatc attccattca catccccgcg ccaaaatgaa gtgtgaagtt tgcgaataac
     4981 tagtaactaa taactagtaa caaataacca atcaccggtt tccctaacgt cctttgaaca
     5041 attcaggtcc tgccagtccc gccttgacac tttcaatggt tccaacacca gtccggctac
     5101 tgagaaagat tagccgctcg atggatccgg tctccaggat ccgctttaaa cccggcctga
     5161 gcatagcctc ggttaaattc ctggcatggg ccgtagcaat aaaactcact ccggcgttga
     5221 tgatctcctc gatcaccgga atatccgtct ccctgccgac ttcatcggta gcgatcacct
     5281 gaggattcat cgcccgtagc aacaggtaaa ccccttcctg tttagaacaa ccatccaata
     5341 tatcggtgca gggcccgaca tccagctgag cgactcccaa ataactcccg gcgatctcgg
     5401 agcgttcatc caccaaccct acatggagcg gcgggatttg caaacattcc accccgttac
     5461 taatctcccg gatgatctcc cgcagtaatg tagtcttgcc cgctgccggc ggggcaatga
     5521 ttaacgtttt tagcaaatgg ccgtccttcc aaagaaccgg tagaatatta cgcgcaatcc
     5581 cccgaatgga tcgggcaatc cgaaaattaa tgctggagat attcttgatt aagcggattt
     5641 tgccgcaatt caccaccgcc tggccggtga ctccgatgcg gtgacctccg ggtaaggcca
     5701 gataacctcc aagcagttct tcctcgacgg cataaaaaga accagtcgtc accgcgttta
     5761 atagttgttt tatatccccg ggtgacaccc ggtacccact ggccaaatcg gaacggagct
     5821 ctccttgggg tgtcacccaa ttactttgct gtcccaggtt gagttccaga gattgattga
     5881 tccgaagccg gatctcaatc acctgttgct gaagatccgg aggaattgcc tgtaacaact
     5941 ggcgaaggct tggagggaaa aagttcaaaa ccccggccca atgcggtaac accttcgccg
     6001 cggcgaagga tgagatagac tcagttccca tggcgccatt cttgtactcg ggatcattca
     6061 ccggctccac tccttaaaag aaaacagccc gtcctggttt atacctatgt agacgggctg
     6121 ttttttatga ctcggctctt ttggggggca atggttaacc atactgtaac cggcaaattt
     6181 gaatggattt cattatatct tttaactcag atcctcggtt ttttccggcg gtgtattctc
     6241 agccataaaa cggctccaaa caactttgcc gcattccggg cagaccactt cataattacc
     6301 gggttcgtcc tcgaaataga tctcctcgtt acaatgaggg caagccattt ccatcaccgg
     6361 ttttccgtta tcttcactaa agacgacatc cgtatcttcc tcatcggaat cggatgtgtt
     6421 gtagaagtat ttctcaagcg agctcaggtc ctcatcaatg gcctcgatat attcggcgaa
     6481 ctcattctgc gactcatcca tctcgccgaa atcggcggca atgccgtcgc agaaatccat
     6541 caacccttcc cagaccaact tctccttttc atttttagga aaatcagctc cttcgatcag
     6601 accgcgcaaa taagcggcct ttgccttgag atcactcaca attatcttcc cctttcgagt
     6661 agactggatg aattgggttg cccaaaactc aatttttcaa acgaagggag tattaaaacg
     6721 cattctatag tttccctgat cccgggtttc ttataccgaa gtaaattgat tgatattata
     6781 aaactggtta aatataatga agataagaaa gttcagccgg gagtccaaaa aaagaacctc
     6841 catatgtccc ggaacaatcc atcaatccga tccggaatac taaaagacaa aaaatatacc
     6901 tttgaccatg agaataatga gtacagggac aatagtgcca ttgacaaaac agttcgcgaa
     6961 caaaatgaat agtcataaaa aacattgaaa tcaaaaagac cgtatcggta acaacccgct
     7021 gcggtcttgg tcaatcgatg aatatgtcta ataaaggtta cttggtaatc cgctttaaat
     7081 attggttcgt ccgggtatct acctggatct tgtccccggt gaccacgaag aaaggcacgt
     7141 tgaccactac gccggtctcg agcgtggcag gtttggaacc gccggtggcg gtatcacctt
     7201 taaaggtagg atcggtctcg gtcacttcca attccacagt attggggaga tccactccca
     7261 atatgtcgtt ttggtatatt tgtatctcga ccagcatgtt ctcttttaag tactttgggg
     7321 catcccccat cttggatgcg ggaatggtca gctgttcgta attttccgta tccataaagg
     7381 tgtaatcatc atcgctcttg tataagtact gcatttcacg gaaatcaata tgggcggctt
     7441 ccactttttc gttggagttg aaagtccgtt ccaccgtgaa tccggttttg atgttcttca
     7501 gtttactccg aacaaaagcg gccccttttc cgggtttgac atgcatgaat tcaattacgg
     7561 tatataaaac gccgtcaatc tcgattgtca tgccagtctt gaaatcagtc accgaaatca
     7621 tctaagtttc ctcccttaac attcgccaga tctacaaaac caatgaattc ttttttaagc
     7681 acgccaatat tttaccatag aaaataagac tttgtcgaga gggttgccca tgttctatta
     7741 taactcgatc agattctgtt tgggactatt ggtcaaatta cggatcccgt cggtctcaac
     7801 caccactaga tcctcaatcc ggactccgcc ccaaccggga agataaatac cgggttcaac
     7861 cgtaattact gcccctaccg gaatctcatg agtttcagta agcgaaagtc ggggttcctc
     7921 atggatctgt agaccaacgc tatgacccag gccatgtcca aaatttgctc cgtacccccg
     7981 cccggcaata taatcccggg ccaccgcgtc gaccttgctc ccgatcatcc cggcccgtaa
     8041 aaccttgagc gcccgttctt gagcctctaa aaccgtttgg taaacctcat gttgttttgg
     8101 atccgccttc cccaggacta ccgttcgggt gaggtccgca caatacccat gataaagggc
     8161 tccgccatcc aaagtgacga gctcacccgg agcaatggtt tttgaagaag ccgaagcgtg
     8221 gggcagcgcc ccccgaaaac ctgaagctac aatggtggta aaggagttcc cctcggcccc
     8281 gttctttcgg atttggtaat caaactccag agccaactcc ctttcctgga caccggcttt
     8341 gattaactgc attgttgcct gccaagcccg atcggtgatg gcctctgctt gagctaaaag
     8401 ttcgatctct tccgcatctt taatccagcg gatctctgca accatctccc ccgcggggat
     8461 caattcctgg cctgccggga taagctcacg cagcaattgg tactccccga aggttacctg
     8521 agcggattca aaaccgatcc tgcgccattt catctcggta agaagtctgg caatggtcgg
     8581 taagagcttt tcggtctgtt tatgtagagt aaatgccttg acttcctggg ccacctgttc
     8641 ccaataacgg aagtccgtaa cgatgactgc agtatcggcg ccgatgagta atgccccttc
     8701 tccaccgctg aagccactca aatatctctg gttttgcggc gcggtgatta acatcccgtc
     8761 cagatccatt ttttgcattg cttcccgcaa tttcgtcaga cgatccattc ttcttcgact
     8821 cctttgacta atgtttgttt ccgaccgtct tgccactcag gagatcctgc agcgcccgaa
     8881 gacccaggag ataactgtta tatccaaaac cggcgatctg tcccacggct atgggcgcaa
     8941 ttaccgaatg gcggcggaac tcttcacgac tataaatatt actcagatga acctccacca
     9001 ccggcagttc cacagagacc aaggcgtccc gaagtgcgta actgtaatga gtatacgccc
     9061 cgggatttat taagataccc tcggcccatt ccgcctcggc ttggatggaa tcgatcaacc
     9121 cgccctcgga gttttgctgg aaaatcttca cttcacagtc catctcggct gcttccaact
     9181 gaattaaacg gttgatgtct tccagggttt gactgccgta gactccaggt tctcgttttc
     9241 cttgccagtt tagattagga ccatgcaata ccaaaatgtg atgcaattaa ctccccccca
     9301 tcgcctgtaa cacctgtaac accaatttct ccggtacatc cccggatata acggcttcgc
     9361 ctaaacggcg cggtaatatc catcgtactt ggcccgatat tgctttttta tccatccgga
     9421 tggcttcaca gatcggagtc gacggtaaat cgaccagcgt agtgggcaga cctaactcgg
     9481 caaaaacctc gcgtaaactg ttgccaagtt cgggctcttc caaaatcccc aaggcctttg
     9541 caatatctac tgcggccaac attcccaagg aaatagcctc accgtgcttg aaacgggtgt
     9601 aatgggtcac gctttccaag gcatgaccga tcgtgtggcc aaagttaagc agcgccctgg
     9661 aaccggattc acgctcatcg ccggcaacaa tcgccccttt gatccggcag gagccgctga
     9721 caacccaggt catgatctgg ggttccaccc ggccaatctg tggtaattca ccgcttaaga
     9781 accgaaaata gttcccatcc aggatgatgc cgtgcttgat aacctcggcc atacccgagg
     9841 ccaattcccg gtggggcaaa gtttttaaga ccgcaacatc cgctacgacc ataccgggct
     9901 ggtaaaaaga tccaattaga tttttgccac gggggtggtt gaccgctact ttcccgccga
     9961 tgctcgagtc cacttgggct aggagcgtgg tgggaatttg aatgaacctc actccccgca
    10021 tataagtagc tgctacgaaa ccggtgagat ctccaatgac tccgccgccc aaagccaaca
    10081 agagtgaatg ccggtcgtat cctcccgcta ccaatagatc ataaagccgg tttgcctctt
    10141 caagggattt ggtggtttcc ccgtccggga ccattgcaaa ctccacggaa aaaccggatt
    10201 ccgtcaagga gtgtaaaagt ggctcttgat accactccgc cacggtcgga ttggtcacaa
    10261 ccaggacctt tccgtccagt ccctgctccc gtatgagtct gccggtccta gcgattaatc
    10321 cctgatcgat atatatcggg taactgcgtt cacctagatt aactcggacc tcttccatca
    10381 cctatctcct ctatccagac acggataaat taaactttag tcaaacccga tcccatttgg
    10441 gatcgagccc cgccaccgcc tgatatccat gagccatgta tagcgcgtct aggatggcga
    10501 tccgaaccat tgcttcgcac accgggtaga tccgcgggca aagcgatgga tcgcggcgtg
    10561 taatcggttg cagggttaca tttctcatct gtttcatatc caccgtggtt tgaggtaccg
    10621 acacggtcgg agtcggtttt accgccatcc ggatccgaat ctcctcaccg ttggtgatac
    10681 cccccaggaa accgccgcta tggttggtcg taaaccggac ccggccggag ctttcatcaa
    10741 aatacggtaa atcattggac tgggatccgg tcattcgggc atgttcaaag ccggcaccga
    10801 actccaatcc cttgatagcg ccgatcgaca tgatcccgtg ggcgatggtg gctgtcagct
    10861 tatcaaagac cggctcaccc aagcccgcgg gtactccatg ggcgattact tccactaagc
    10921 cgccgcaggt atctccggcc tcttttaccg ccaggatgtc agcaatcatt ttttcagcca
    10981 tttccagatc ggggcagttc aaatcgttct tcctgtaatt ggccttggca atttcgtaac
    11041 ttataggtcc ggccttgatc ccgtgggatt cggtgacaaa cccaatgaca tcaatgccga
    11101 ttctgtccag gatcgcctga gcaatggccc ccccagcgac cctgctggcc gtttcgcggc
    11161 ctgatgctct tccggcgccg acccaatcgg cgtattcgcc atattttttg aagtatgtat
    11221 actcggcatg acccgggcga actacatcct gataactccg gtactgttcg atatggacgt
    11281 cctcaatgtc cacattcgga ataaccaggc ccaccggggc accggtggaa agatcatcct
    11341 ccatcactcc ggaaaagata tagaccctgt ccgtctcttt ccggggagaa tccaaggctg
    11401 accgccccgg ccgtctttta tccaattcct tttggaccaa ttccgcggtg atcttgaccc
    11461 ccggcggtac cccgtcgaca atggtcatca acccgccgta taactctttc ggaatgtcca
    11521 attgtttccg gaaacctccg gcgtaagatt caccacaggt ggtaatccga aagaccctac
    11581 caaacgtatt tcccatcatt acgaccaacc tccatctttc cacataaaac ccgtttaaag
    11641 agatttcgcg ccccgtgggg cgcgctactc atgcctcatg cctcacgcct attacccttc
    11701 cgccataagc cgaatcttac cgccacatgc ggagataata tcgacaaact ccggaaaagt
    11761 aaccctaacg gcctcggcgc tatcgatgag ggtaattccg gaagcgttca gtccggcgat
    11821 agccaatgcc atcaccactc ggtgatcgga atggccattt aagaaacaac ccttgagcga
    11881 actggacttg atgacgagac cgtcgggaag ctcggtgata tccgcgccca tcttgacgag
    11941 ttcggcagcc atcacccgaa tccggtcggt ctctttgagg cgggcttggg gtacattgac
    12001 caaccgcgtc tcaccgctgg cgaaacaccc cgccacagcc aaggccggaa gcgcatccgg
    12061 aatggcgttg agatcgatct cccggccttt gaggccattc cctacgatcc ggattgtgtc
    12121 cccggtcacg gtaacattag cgcccattga ttcaagtacg ggcaagatcg ctttatcccc
    12181 ctgagtatct ttaagatcta agttttccaa gatcatctca gaccctgata ttgcagcctg
    12241 caccattaaa aaagtggctg atgaaaaatc cccgggaatt gtggtacgaa aagcctgata
    12301 gtgctgaccg cccttcacgg taaactgccg ataaccgtcg cgatcataac tgatcctttg
    12361 tccatcaagc caacccagag tcatatccac ataaggggct tcattcagcc gggtgacccg
    12421 aatggtagta tccccatcca aaagcggggc gtgaataagg agcgccgaca aatactggga
    12481 agtaaccgca tcgacagaga tcgcaccgga atgaacgcga cccttgatga cggccggggc
    12541 agacccgtta tcccgggtgg tgaaagcaat tcctcccaag tcacaaatgg ccgttagtaa
    12601 tgggcccagc ggacgactac ggatctgctc atcccctgtg aatacggtgt atccatcgca
    12661 cagcgcagcg gtcatgactc caaagcgaag ggtagtcccc gagttaccca cttggattat
    12721 atcctgtggg acctgtggta aaccgttaat accttcgata gtgaattttt gttcttcctg
    12781 aatgattcgg gcgccaaacg cctgacaggt agtgactgcg gaaagagtat cgcttgagat
    12841 aagcggggca tgaatctccg acctgccttg agccaagctg gcgaggaaca atgccctgat
    12901 ggtctgagat ttggagccgg gtatgcgaac tcgccccccg gcttgggagt tggagacttt
    12961 taataacatg aaaaaacacc tcgctaccgt cctacaatgc tgctacttta atttacaata
    13021 ataatataga taacaatgga agtcaaacct tttttccgcg cgctcgtaca agggtttaca
    13081 ataacgtgaa gacaccacaa acgtgaaaac accacaactc attcccgatg gaaggttttt
    13141 acggaaaaat gtagaaacaa cttgtttaac ttatatggtt tacttaaggg aggttctttc
    13201 gatgactcat gtttttcgcc aatacgacat ccgcggcgtg gtggattccg atctgagcat
    13261 tgatttcgtg gccgatttgg gtaaggccat cgccgctttc ttcaaacaac gtaaagtcga
    13321 gacagtcagt ttgggtttcg actgccgtct tagctcgatt ccttttcacg atcgcctggt
    13381 tgagagcttc gtaacctcgg gattaaccgt atatgatatc ggcatggtcc cgacacccat
    13441 cgtctatttt tccctcttca atctgccggt cgacggtgga gtaatgatta ccggcagcca
    13501 taatccgcct aacgaaaacg ggtttaaaat ctgtctcggg aagtctacca tctttggtga
    13561 ggatattcag gagattgggc ggatcatgga tgagcgccgg tttatatcgg gccaaggtcg
    13621 tttgatcgag cgctccttga tccccgatta tctctcttat gtacaaaaga tcatccatcc
    13681 cggcccaaag cctctcaagg tcgtggtcga cgccggcaac ggcaccggca atcttacctc
    13741 agttccccta taccggagcc tgggttggga agtcattgag ctctattccg aaccggatgg
    13801 gaactttccg catcatcatc ccgatcccac ggttcctgcc tatctcgtgg acctaaaggc
    13861 aaaagtggca gaaatcggcg ccgatctggg tatcgccctg gacggcgatg ccgaccgaat
    13921 cggagtgatc gacgccaagg gtcgtaccct gtggggcgat caattgctcc agatctacgc
    13981 ggccgagatc cttcaggcta aacccggtgc caagatcatc ggtgaggtga aatgctccca
    14041 aaagctattt gatcgcatcc gggaactggg cggggttccc atcatgtgga aggttgggca
    14101 ttcgctgtta aaagccaaaa tgaaagagga acaagcggtt ctggccgggg agatgagcgg
    14161 ccacctcttc ttcgccgatc gctattacgg ctatgatgac gcagtctatt ccggagccag
    14221 attgcttgag atcttgtccc attcgactaa gtcattggtg gagatggtcg atgcattgcc
    14281 aaaaacctat gccactcctg agatccgttt tgaatgcccg gatgatcgta aatttcaggt
    14341 agtccgcgac ctaaccgaac acttccgcaa ggagtatcaa gtaatcgaca tcgacggcgc
    14401 ccggatcatc tttccggatg gctggggctt ggtaagagcc tccaataccc aacccgtgct
    14461 ggtactgcgg tttgaggccg attcccaaac cgccctgacc cggattgaag ctgaagtccg
    14521 cgccgagttg gatgtgcttg ttaagtaaga gacgactcac tcttgctctc tctagcggcg
    14581 agcgcta
//
LOCUS       DASVMB010000093        13742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_453690,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000093 DASVMB010000000
VERSION     DASVMB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453690"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF531_03075"
     CDS             complement(<1..586)
                     /locus_tag="VF531_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7712979.1"
                     /translation="MNSAADIRKTGEKVINRLIKTTKNDGSNDHLVIETWEWPQGVAL
                     YSLFKYDQVTGEGIYLDFIRDWYPKRLAEGFPARNVNTTAPLLTMAHLYELTGKKEYL
                     EICSAWAEWVMKEMPRTQEGGLQHITAIEVNREQLWVDTLFMTVLFLAKMGRILKSEA
                     YIEEAIYQFLLHIKYLYDPATGLWFHGWTFQDRNH"
     gene            complement(881..2506)
                     /locus_tag="VF531_03080"
     CDS             complement(881..2506)
                     /locus_tag="VF531_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7712980.1"
                     /translation="MMMLSYTTNLNYFKRLGSVLGGHDLSYEELQLLRFCQDTLDISA
                     GIHPYIYSVYCLPYSKRSDSTFMVSFDGVYSLTNYPDRSFITSVRHSTRDYGIVVRNY
                     KSGSLPAVKLLSKYALVKSVSHNRTEGILIVNLDIKYIELMLNKYMHERTDGLFIVSW
                     NRHTPIFTSTELKKLGSDQVKEMLRQVEAGHSVFLPASNDPTISGMPRKVEYLPVKEI
                     TSNYGFTVHLMVSRASLYATSQNLVFANIVVILFSIFIGIAIIIMITQKRYNDIIKIG
                     DYIDALDETGSVTAIPPKSGKGLDKTISGFHQFTDYVKLRLSERELKERKLEMQLLRS
                     QLNPHFLLNTLQMLNWKIMREFKGHSDFNTIIENLCKILSYSLYPAESLAPLAEEIRY
                     TDSYVALQRNNQKYSIQIFWEVDDSMKQYMVPRLLFQPIIENAQKHAFSKEFAEHEKA
                     LSEPQITIRFERRYGDLYIGVKDNGYGMDGETLEKIRDSLSREPVNVSGIGLSNTHKR
                     IQLLFGRKYGVTVNSILGEGTEVVLRIPILLQD"
     gene            complement(2730..3800)
                     /locus_tag="VF531_03085"
     CDS             complement(2730..3800)
                     /locus_tag="VF531_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7712981.1"
                     /translation="MLLRNTGQDADDGKRNKMGSGVKDSTASQASGAMTAVDDHLTLN
                     NWEWPTGVALYSMYKLADRSGDREFYDYLLQWYDSKLLKSDVHKNVNTVAPMLALACL
                     YEDEPRPQWRVKIQEWVDWIMLEMPRTTYGGLQHMTVLNRHYQQLWDDTLFMTVLFLA
                     KAGGVLGCETLVDEAKYQFLIHIKYLQDKLTGLFYHGWTFDYKHNFANVFWARGNSWF
                     TAGAVEFLEICGNRDSVFRYVLSCLEDQVKELVKHQGENGLFTTILDSEESYSETSAT
                     ANIAYGILKGIRLGLLDGGYAEAGQKAARAVIDQISGDGTVQGVSYGTGMGTSADHYR
                     KIPIRPTAYGQGLCLLLLSELV"
     gene            complement(3885..4631)
                     /locus_tag="VF531_03090"
     CDS             complement(3885..4631)
                     /locus_tag="VF531_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7712982.1"
                     /translation="MYQLIIIEDEIEIRKGFVECLPWAELGFEMVNDFACGKPAIAWL
                     ENHTVDVVVTDVKMDEGSGLDVAEYLNKAGRLETVVFYSAYKDFDYARKGMNFGVKRY
                     ITKDMDYNELVEIFRQVKSDLDKEINGAPKVLASPTPEVKPHNNPIVNQVLRYVAKSY
                     RTATLESAAKVVQLNPYYLSVYIKSKTGENFRDILTRVRMEKAKILLQDPTYRICEIS
                     DLLGYSDVRNFVRRFKSYYGTIPSIQKKNQ"
     gene            complement(4687..5697)
                     /locus_tag="VF531_03095"
     CDS             complement(4687..5697)
                     /locus_tag="VF531_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7712983.1"
                     /translation="MNDDRHDDGQMRFDLLVVGGTLEGCIAAVVAARSGYKVVLIENS
                     GSLGGLATNGLHSWFPALDSQLGDTTVNQIRNELLERLEIAISEAPALYREQKLKVIL
                     GRMLRDNGITLLTHVFVSSPIIEAGGLKGFHVYGKTGCIQIYAGVVVDATEKMECPGQ
                     LDLEMINNQKAVNIGMKLNTVNFGEITGLCGTKIWDNGKSFVAKLSVPYSWQHGGMTM
                     ETAELLLLGDADCGELIVHGLKCSTDTVDPLELSKIQMNLSRFAYKLRDYLRKTVAGF
                     EAAHIIHIAPKMDTYGLRCFDEGRLIYKNLLVCNNHAGAYGNNEAIRIGAIAGKCAAS
                     IG"
     gene            complement(5726..7243)
                     /locus_tag="VF531_03100"
     CDS             complement(5726..7243)
                     /locus_tag="VF531_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7712984.1"
                     /translation="MESEKVMIEVDVLVTGGGVSGCAAAIAAARAGARTMLIERNGFL
                     GGIASSSMISNIYNHYIARDGRMVMRGIGVEVANRLVNRNAGTPEWKYPDGRLVHDPE
                     QLKLVFNEMMDEAGVTVLLNSLAIDPVVEDGKICGVHVDTAAGRRVIRAAVVVDTTGE
                     CDVAWSAGVPVRKAAGLGTLTFKMANVDLEKLYLHFKAHPETFPVGIDAMKDFKEFEN
                     VWLNRGVLYFPHSGGEDWDLFQNAIQRGKFMKEKGNIFGMDSACIIGMKGHNTAVINS
                     QFWRIVSIDPRIMSPAEVEVHEACLYVADFMVRNIPGFEHAYTAQISDEIAIRVSRGI
                     IGEETFDKDERTVRNVIESDDAVANGTDIEYRILRDIHADPRYVKSELKEEDNIRVDD
                     VIACRPIQKNFKLTGEFVSNFTVDIPYGVMLPLNVENLLVASGKGVSCVPQTMLRYQS
                     AGMALGQAAGVAGALAVKNGVTVRNLDIRLLQYGLLDQDVYLGDEKRLCELGLMR"
     gene            complement(7266..8093)
                     /locus_tag="VF531_03105"
     CDS             complement(7266..8093)
                     /locus_tag="VF531_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7712985.1"
                     /translation="MKKNFWAKILLFLLAVIVSYPFIWLFTASFKNNTEIFASPSLFP
                     QQFTLEGYINGWKGIGENTFALFLNNSFWLVIPTVLLTVVSSCLVAYGFARFEFPLKK
                     FWFAVMISTMMLPNTIIMIPRYILFRDFGWLDSYRPFFALAALAANPFFTYLLVQFFR
                     GIPIEMDESAYMDGCTRRGTLLRIIIPLAKTPIISVVLFQFIWTWNDFANPLIYVNST
                     KLYPVSLGLRLAIDSESAISWNKIIAMSVVSMLPCIALFLSMQKYFVEGIATSGLKG"
     gene            complement(8097..8987)
                     /locus_tag="VF531_03110"
     CDS             complement(8097..8987)
                     /locus_tag="VF531_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7712986.1"
                     /translation="MRLSRNTLEKFHGYLYIAPWLIGFFLLTLIPFVASFVYSFTNYD
                     MFNFKFVGLRNYIDIFTNNQIFRDSLGRTLLYTLIAVPGKLVVALILALLLNKKIKGI
                     GVYRTLFYLPSILGASVALSILWKYVFKTDGLANMFFGLFHMPGINWLGDPDKALFCL
                     ILLPLWQMGSPMVLFLAALKTVPSEIVEAATIDRAGKIRIFFHVTLPLITPVIFFNLI
                     MQTIEIIQLFTPAYIITQGGPIKATYLYALMLYDVSFRDFQMGYASALSWVLFVIIII
                     YTIVLFGTSRKWVFYEDEGA"
     gene            complement(9073..10371)
                     /locus_tag="VF531_03115"
     CDS             complement(9073..10371)
                     /locus_tag="VF531_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7712987.1"
                     /translation="MRKSMLIILSLMLILALIIPVSANSKSVTLRFSWWGGDTRHQAT
                     LEAIKLYMKLNKGVTIEPEYSGFSGYYQKLVTQLASDTAPDIFQSDQGWTTEFNNRGD
                     VFANLNDLKSYLHTNKLNKSMLDDYCVRDGKLVVLPLGYNGTVFLYNKSLMEPYIAPY
                     LKNGKLQLTWDDLIKIGQEMHAKNKDVYLMTNITDGYVRFMLKAILKQITNKIPIQDD
                     YTIGFTKADMTKAFKKMLEMFDSGAAMPYSESVLYKDSLVNNPKWMSGQIGGAFIFFS
                     NIDAWTNGIKYNFDVTSLPKFADAKTSGQESAPSLMIAINNRSRNKKEAAKFVNWFLN
                     DPKCAAILKTERGVPTNSDALNVLKKNNTLSPIMAKAIATSDATRGFKNGALEMNASI
                     HAIFVEKMEKVLYKIVTPEQASEELINDLTAKLKEMKGQE"
     gene            complement(11020..11496)
                     /locus_tag="VF531_03120"
     CDS             complement(11020..11496)
                     /locus_tag="VF531_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7712988.1"
                     /translation="MSKIFIDTNILVYAMDGADPLKQKQSRFLLQELQENSNTGVIST
                     QVLQEFYVTATKKLNLDPILVKSILRAFENFEVVVIGPDLIESAVDCSVLNQISFWDA
                     LVVVSAESACCERILSEDLNSGQIIRGVKIANPFFKPDRLNEAGEFYQLKTPKRRC"
     gene            complement(11489..11713)
                     /locus_tag="VF531_03125"
     CDS             complement(11489..11713)
                     /locus_tag="VF531_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEX7712989.1"
                     /translation="MKNITISLDDELLKEGREYARRHNTSLNALIRKLLAQTVLPNSQ
                     NWLEECFALMDQANANSRGQKWNREDLYDV"
     gene            complement(12055..13041)
                     /locus_tag="VF531_03130"
     CDS             complement(12055..13041)
                     /locus_tag="VF531_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7712990.1"
                     /translation="MKIIQVRDLRKTYLTYERGSSFLETVKSLVVRKPIAIDAVKGIT
                     FDVEQGELLGFLGPNGAGKSTTLKILTGVLFPTAGEVDVMGYTPWRQRRKYVANIGAV
                     FGQKSQLIWDIPPLDSFIMNKAIYEIADSDFKKRLEEMINLLGVEGVIRKPTRQLSLG
                     ERMKCEFIMAMLHNPKIVFLDEPTIGLDVIAKDRIREFILERNRTGTTFILTTHDLDD
                     VERLARRVIVVNHGEIVFDDGLEALHGFSGHQKFVSLSTQKPLPDFTTMPGVTVTGQP
                     SQYNAELELDMGKIELNCFIQAVNESYVINDLTIGEPPIENIIKELYMKKNE"
     gene            complement(13052..>13742)
                     /locus_tag="VF531_03135"
     CDS             complement(13052..>13742)
                     /locus_tag="VF531_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX7712991.1"
                     /translation="MIMVAELLIPFLTLLIYGTGASFPGWSIYEMLLLQGIFMLSRGV
                     AGLLFFGVVWNTLDRVREGTYDLLLIKPRSVLFLAVATGFEPDSIGTIAGGLMVMGFS
                     LRNLTAPGMWEWLEFLVLFLFSVMVLFSFALFMAGSVFKWVGNSRVYEIFDSTTAFGN
                     YPRSIFSRWFQAIISYILPLAAIGFFPAAVLLGKPTDGIFATMFVCLVFVGLSLAFWR
                     WMLSKYTSAGG"
BASE COUNT         3850 a         3448 c         2769 g         3675 t
ORIGIN      
        1 aatgattccg gtcttgaaaa gtccatccgt ggaaccataa tccggtcgca ggatcgtata
       61 aatacttaat atgtaataag aattgataaa ttgcttcctc gatataagct tccgatttaa
      121 gaatccggcc catcttggcc agaaacagca ctgtcatgaa gagcgtatcc acccataatt
      181 gttctctgtt aacttcaatg gcggtgatat gctgaagccc gccctcttgg gttcggggca
      241 tctccttcat tacccattcc gcccaggcag aacaaatttc cagatactct ttctttccgg
      301 taagttcata taagtgggcc attgtcaata agggggcagt ggtattgaca ttcctggcgg
      361 gaaatccttc agccagcctt ttggggtacc aatcccggat aaagtccaga taaatcccct
      421 caccagtcac ctggtcgtat ttgaacaaac tgtacaacgc cactccttgg ggccattccc
      481 aggtctcaat caccaaatgg tcgtttgaac cgtcattttt agtggttttg atcagccggt
      541 tgatgacttt ttctccggtt ttccggatat ccgctgccga attcatggta gttcctccgt
      601 ttgcgctcca ataaattccc attattgtat aattcactat catcgaatga catcctgcca
      661 aatatccaaa tttcctattt gcatagctta tttatcccca actacaaata aaataggcct
      721 ctcggtgact tatctcaccc gaatggccta cccttacccg cttctaatca ttaatcccca
      781 ctggtggaca gcgtttattt ttgtcgctcc ggcttgagaa agggattctc tatttggcat
      841 tcaacccttt tagggaagat tatgaaatta atcatctccc tcaatcttgc aaaagtatgg
      901 gaatccgcag cactacttca gtgccctcac cgagtatact gttgaccgtg actccgtact
      961 tcctgccgaa aagaagttga atccgcttgt gtgtatttga caggcctatt ccggaaacgt
     1021 ttaccggctc ccgggagaga gaatcacgaa tcttttccaa cgtttcgcca tccattccat
     1081 aaccgttatc ctttacaccg atatacaaat caccatatct gcgttcgaag cgaattgtta
     1141 tctgaggctc cgataacgct ttttcatgtt ccgcaaattc cttactaaat gcatgctttt
     1201 gtgcgttctc aataatcggc tgaaacaaca ggcgcggtac catatactgt ttcatggaat
     1261 cgtcaacttc ccagaatatc tgtatcgaat atttttgatt atttctttga agcgcaacat
     1321 acgaatcggt atagcgtatt tcttcggcta acggagccag ggactccgcc ggatataaag
     1381 aataggataa aatcttgcaa agattttcaa tgatggtgtt gaaatcgcta tgccctttga
     1441 attccctcat aattttccaa ttgagcattt gcagggtatt aagaagaaaa tgaggattta
     1501 actggctgcg gagcagttgc atttccagtt ttcgttcctt aagttcgcgt tcggaaagcc
     1561 gcagtttcac ataatccgta aattgatgga atccggaaat cgttttatcc aaaccctttc
     1621 ccgacttcgg cgggatggcc gtcactgagc cggtttcatc aagggcatca atatagtccc
     1681 ctatttttat aatatcattg taacgtttct gcgtaatcat gataataatg gcaataccta
     1741 tgaatatgga aaacagaata actacaatat tggcgaaaac caggttttga ctggtggcat
     1801 aaagtgaagc gcgtgaaacc atcagatgga ctgtaaaacc gtaattgctg gtaatctcct
     1861 taaccggcag atattctact tttcggggca ttccggatat tgtcggatca ttcgatgcgg
     1921 gcaagaaaac agaatgaccg gcttcaacct gccgaagcat ctctttaacc tggtcactgc
     1981 caagtttctt cagttccgta ctagtaaaaa tgggggtatg ccggttccag gaaacaatga
     2041 agaggccgtc ggtccgttca tgcatatatt tgttaagcat gagctcaata tacttaatat
     2101 ccagattcac gatcaggata ccctcggttc ggttatggct cacacttttg accaatgcgt
     2161 attttgaaag aagcttcact gcgggcagag atccactttt gtaattgcgg acaacaatac
     2221 cgtaatcccg cgtactgtgc cggacagaag ttataaatga cctatcagga taattcgtca
     2281 gtgaatagac gccgtcaaaa gataccataa atgtagagtc ggatctcttg gaatagggca
     2341 aacagtaaac cgaataaata taggggtgga ttccggcgga aatatccagg gtatcctggc
     2401 aaaatctcag caactgcaat tcttcgtaag agaggtcatg ccctcccaat acactaccca
     2461 accttttaaa atagttgagg ttggtagtgt aggagagcat catcatgtcc gtctcttcca
     2521 gtattttatt caaatcagcg cataattgcc gcgcaatgtt ctcacaaacc cgggcattct
     2581 tttaccgcac aaaattattg aggtagcagc ccgatataaa cgtaagaatt accgttatcg
     2641 ctaccggcaa cagaaaactt aaaacatatt taaagaatat atattttttg gatacccgtc
     2701 tcaacatttt aaaactcgca tcgttccgat cagaccagtt cgcttaagag caacaagcac
     2761 aatccctgcc cataagcggt aggccgtatc ggaattttcc ggtagtgatc tgccgaagtg
     2821 cccataccgg tcccgtacga aaccccctga acggttccgt cgccagaaat ttgatcaatc
     2881 acagcccggg ctgctttttg ccctgcttcg gcatacccgc catcaagcaa accaagacgt
     2941 attcctttca atataccata agcaatgttg gctgtggctg aagtctcact gtaactttcc
     3001 tcggaatcga gtatggtagt gaacaacccg ttttccccct gatgcttcac aagttcctta
     3061 acctggtcct ccagacaact caacacatac cggaatacgg aatcccggtt accgcaaatc
     3121 tccaggaatt caacagcgcc ggcagtaaac cagctgtttc ctcttgccca aaacacattg
     3181 gcgaagttat gtttgtaatc aaatgtccaa ccatggtaga acagaccggt aagcttatcc
     3241 tgcaaatact taatgtgaat cagaaactgg tatttcgcct catccactaa ggtttcacag
     3301 cccagtaccc cgccggcttt cgcaagaaac aaaaccgtca taaacaacgt atcatcccag
     3361 agctgctggt aatggcgatt cagaaccgtc atatgctgaa gtccgccata ggtggtacgc
     3421 ggcatctcaa gcataatcca atctacccat tcctgaattt tgacgcgcca ttgcggccgt
     3481 ggctcgtctt catacagaca ggctaaagca agcataggcg caaccgtatt cacattcttg
     3541 tgaacatccg actttaataa cttggaatca taccattgta gcagatagtc gtagaactcc
     3601 cggtccccgg aacggtcggc aagcttgtac atactgtata aagccacgcc ggtaggccat
     3661 tcccagttat tcaatgtcag gtggtcatcc acagccgtca ttgctccgga tgcttgggaa
     3721 gccgtactat ctttgacacc gctgcccatc ttgtttcgtt ttccgtcgtc agcatcctgc
     3781 ccggtgtttc taagcagcat cgacaggacc ttccgggcaa gttcagtata attcaaggaa
     3841 tcgtcattta tccccataag ttttcacctt taccatgtag ttggttattg gttcttcttc
     3901 tgaatactcg gtattgtgcc gtaataactc ttgaaccggc ggacgaaatt tcgtacatcg
     3961 ctataaccca gcagatcgct tatctcgcaa atgcggtatg taggatcctg cagcaatatt
     4021 tttgctttct ccatgcggac acgggtgaga atatcgcgaa agttttcacc tgttttactc
     4081 ttgatgtata cactgagata atagggattc agttgcacca cctttgcagc gctttccaac
     4141 gtggcggtgc gatatgattt ggcgacatag cgtaagacct gattcacaat gggattatta
     4201 tgcggtttca cttccggggt tggagaagcg agaactttcg gtgcgccgtt gatctctttg
     4261 tccaggtcgc tcttgacctg gcgaaaaatt tctaccagtt cgttgtaatc catatctttg
     4321 gtgatgtaac gtttcactcc gaagttcata cctttgcgag cataatcaaa atccttatac
     4381 gcactgtaga acacaacggt ctccaatctc ccggccttgt tgagatactc ggcgacatcc
     4441 agaccgcttc cttcatccat cttcacatcg gtgacaacaa catccaccgt gtggttctcc
     4501 agccacgcta tcgccggttt accgcaggca aagtcattca ccatctcgaa acccaattcg
     4561 gcccatggca aacattctac aaatcccttg cgtatttcga tctcgtcttc gataataata
     4621 agttgataca taatattatt atgcaccccc cgagtcgagg ccgtcaaatg taaataaaat
     4681 acaagttcat ccaatgctgg ccgcacattt gccagcaatc gcgccaatgc gtatcgcctc
     4741 gttattgcca tatgcacctg catgattatt acataccaaa aggtttttat aaattaatcg
     4801 accttcgtcg aaacatcgca atccgtacgt atccattttt ggagcaatgt gaataatatg
     4861 ggcggcctca aaacccgcaa ccgtttttcg cagataatcc cgcagcttgt atgcgaaccg
     4921 agacagattc atttgaattt tggaaagttc cagaggatcc accgtatccg tactgcattt
     4981 aagaccatgt acaataagct ctccacaatc ggcatcccca agcagtaata actcagcagt
     5041 ttccatggtc ataccgccat gttgccagga gtatggaacc gacagcttgg ccacaaacga
     5101 tttaccgtta tcccaaattt tcgtgccgca aagcccggta atctctccaa aatttactgt
     5161 gttgagcttc attccaatat tgacagcctt ttggttattg atcatttcca gatctaactg
     5221 acccgggcat tccatctttt cagtggcatc aacaaccaca ccggcgtaaa tttgaataca
     5281 tccggttttt ccataaacat gaaatccttt gagcccacct gcttcgataa ttggcgacga
     5341 cacaaacaca tgggttaaca atgtgatacc gttatcgcgc aacatccggc caagtatgac
     5401 ttttaacttt tgttccctgt aaagcgcagg tgcttcagat atcgctattt ccagccgctc
     5461 cagcaattca ttcctgattt ggttgactgt ggtatcaccc agctgagaat caagcgccgg
     5521 aaaccaggaa tggagaccat tggtggccag gccgccgagg gaaccgctat tttcaatcaa
     5581 aacaacctta tatccgcttc gggcggcaac aactgcggca atacacccct ccagcgtacc
     5641 accgactaca aggagatcaa atctcatttg cccatcatca tgccgatcat cgttcattcc
     5701 tgcatacccc ctgttttaat cggaattatc gcatcaggcc caattcgcac agcctttttt
     5761 catctccaag atacacatcc tgatccagta atccatactg caatagccgg atatccagat
     5821 tccggacagt aacaccattt ttaacagcga gggctccggc cacaccggcc gcctgtccca
     5881 gagccatacc ggccgactga tatcgcagca ttgtctgggg aacacatgat actcctttgc
     5941 cgcttgcgac caaaagattt tcaacattca agggcagcat gactccatac gggatatcaa
     6001 cggtaaaatt acttacaaat tcgccggtga gcttgaagtt tttctgtatg ggacgacagg
     6061 caataacgtc atccacacga atattatctt cctctttcaa ttctgatttc acataacggg
     6121 gatcggcgtg gatatcacgg agaatccggt attcgatatc ggttccgttc gccacagcat
     6181 catcggactc aatgacattg cgtaccgtcc gttcatcctt gtcgaatgtt tcctcaccga
     6241 taatcccccg acttacccgg atggcaatct catcggatat ctgcgcagta taggcatgct
     6301 caaacccggg aatattccgc accatgaaat cagctacata aagacaggct tcatgtacct
     6361 ctacctcggc tggcgacatg attctcggat caatagagac aatacgccaa aactgggaat
     6421 tgatcaccgc ggtattgtgc cccttcattc cgatgatgca tgcggaatcc ataccgaaga
     6481 tgtttccctt ttctttcatg aacttaccac gttgaatggc attttgaaac agatcccaat
     6541 cttcgccgcc tgaatgagga aagtacaaaa caccccggtt gagccagaca ttctcaaatt
     6601 ctttaaagtc cttcatagca tcaataccta caggaaatgt ttcagggtgc gccttgaagt
     6661 gaagataaag tttttccaga tccacattgg ccattttaaa agttaacgta ccaagccccg
     6721 cagctttgcg gacgggtaca ccggcagacc atgcaacgtc acattcaccg gtagtatcaa
     6781 ccaccactgc cgcccttatg acccgtcgtc cggcagccgt atctacatga acgccgcata
     6841 ttttcccgtc ttcaacaaca gggtctatcg ccagggaatt caacaggact gttaccccag
     6901 cctcatccat catttcgtta aaaaccaatt taagctgttc cgggtcatgt accagcctgc
     6961 cgtcgggata cttccattca ggcgttccgg catttcggtt caccagccgg ttagctactt
     7021 cgacgccaat gccacgcatg accatcctgc cgtccctggc gatatagtgg ttataaatat
     7081 tcgaaatcat actggaactg gcaataccgc ccagaaaacc attccgctca atcagcatag
     7141 ttctggcgcc cgccctcgct gcggcaatcg ccgcggcaca acctgaaact cctccgccgg
     7201 tgaccagtac gtcgacctcg atcataactt tttcagattc catctaatat taccctccct
     7261 taaccttatc ctttcaaacc tgaagtggca atgccttcca caaaatactt ctgcatggaa
     7321 aggaacaaag cgatacaagg cagcatgctg actacggaca tagcaatgat cttattccaa
     7381 cttatcgcgg actcactatc tatcgccagc cgcaaaccga gcgatacggg ataaagtttg
     7441 gtggagttta catagatcaa cgggttggca aaatcgttcc atgtccagat aaactgaaac
     7501 aataccacgg atataatcgg cgttttggcg agcggtatga taattctgag caaagtcccc
     7561 ctccgcgtac agccatccat gtatgccgat tcatccattt cgatcggtat cccccggaag
     7621 aactggacca gaaggtaagt aaaaaacgga tttgcagcca aagctgcgag ggcaaagaat
     7681 ggcctgtaac tgtccagcca cccaaaatcc ctgaataaaa tgtagcgcgg aatcatgatt
     7741 atcgtgtttg gaagcatcat tgtggatatc ataacagcga accagaattt tttaagtgga
     7801 aattcgaaac gggcaaaacc ataggcaacc aagcaactcg ataccacagt aagaagcacc
     7861 gtagggatga ctagccagaa tgaattgttg agaaataatg caaaagtatt ctctccgatc
     7921 cctttccagc cgttaatgta accttccaag gtaaattgct gcggaaacag gctcggagaa
     7981 gcaaaaattt cggtattgtt tttaaatgag gctgtaaata gccatataaa agggtaactt
     8041 acgattacag ccagtaaaaa tagaagtatt ttagcccaaa aattcttttt catgcgtcat
     8101 gctccttcat cctcataaaa cacccatttt cgggaggtac cgaaaagtac gatggtataa
     8161 ataatgatga tcacaaacag cacccaagat aatgcactgg cataccccat ttggaaatcg
     8221 cggaacgata catcataaag catcagcgca tatagatagg tggctttgat aggacctccc
     8281 tgcgtaatga tataagcggg tgtgaataac tgaataatct cgatggtctg cataataaga
     8341 ttgaagaaaa ttacaggagt gattaaaggt aaagtcacat gaaagaaaat acgaatttta
     8401 cctgccctat caatcgtagc agcctcaaca atttccgatg gcactgtctt gagagctgcc
     8461 aaaaacaaca ccatgggcga acccatttgc caaagcggca gaagtatcag gcagaataaa
     8521 gctttgtcag gatctccaag ccaattgatt ccaggcatgt gaaacaatcc aaaaaacatg
     8581 ttggcaagcc cgtccgtttt gaacacgtat ttccataaaa ttgacaatgc cacgcttgcg
     8641 cccagtattg aaggaagata aaacagcgta cgatagactc caatcccctt gatcttcttg
     8701 ttgagcaata atgccaggat aagtgcgaca accagtttcc cgggcaccgc gattagggtg
     8761 tacaataagg tacgcccgag cgaatctcta aatatctggt tattggtgaa tatgtcgata
     8821 taattcctta acccgacaaa tttgaagtta aacatgtcat aattggtaaa agaatacaca
     8881 aacgatgcaa caaacggaat gagcgtcaat aagaaaaatc caatcaacca cggtgctatg
     8941 taaagatatc catgaaattt ttccaatgta tttcggctta gccgcatgat gtcctccgta
     9001 cgtaacactt ctaaaatatc atcgaagaca catccggggt gttgccaccc cggcttgtgt
     9061 cttccgtaaa agttattctt gtcccttcat ttcctttagc tttgcagtca agtcattaat
     9121 aagttcttca gacgcctgtt cgggagttac tattttataa agtaccttct ccattttctc
     9181 tacaaagatg gcgtgaatgg aagcgttcat ttcgagtgcg ccgtttttaa aaccacgggt
     9241 agcatcagag gtagcaatcg cttttgccat tatcggactt aaagtattgt tctttttcaa
     9301 cacattcaga gcgtctgaat tggtcggaac accgcgttcc gtcttgagaa tcgccgcaca
     9361 cttcggatca ttgagaaacc aatttacaaa ttttgcagct tcctttttat tccgtgatct
     9421 gttgtttatg gcgatcatca gcgatggggc ggattcctgt cctgatgtct tggcatcggc
     9481 aaacttcggc aagctggtaa catcgaaatt atacttaatc ccattggtcc aagcgtcaat
     9541 gttggagaag aatataaatg ccccgccgat ttgtccactc atccacttgg ggttgttcac
     9601 cagcgaatcc ttgtaaagca ccgattcact ataaggcatt gccgctccac tgtcgaacat
     9661 ctccagcatc tttttgaacg ccttggtcat atccgccttg gtaaatccaa tcgtgtaatc
     9721 atcctgaata gggatcttgt tggtaatctg cttaagaatg gcttttaaca taaaccgtac
     9781 ataaccatca gtaatattgg tcattagata aacatcttta ttcttggcat gcatttcttg
     9841 gcctattttt atcagatcat cccatgttag ctgtagcttg ccatttttta aatacggtgc
     9901 aatgtaaggc tccataagac tcttgttata gagaaagact gttccgttat atcccaacgg
     9961 caggacaaca agtttgccat cccttacaca ataatcgtca agcattgact tgttaagttt
    10021 gttcgtatga aggtaacttt ttaaatcatt taagttggca aaaacatcgc cgcggttgtt
    10081 gaactccgtc gtccagcctt gatcggactg gaagatatcc ggagccgtgt cggatgccaa
    10141 ctgggtgacc agcttttggt aatagccgga aaaaccgctg tactcgggtt caatcgtaac
    10201 ccctttattc aatttcatgt acaacttgat cgcttcaagg gtcgcctggt gccgggtatc
    10261 gccgccccac caagaaaatc gcagcgtaac cgatttagaa tttgccgaaa ccgggattat
    10321 caaagccagt atcagcatta gactcagtat tattaacatt gactttctca taaggacccc
    10381 ccgttttaat tgtgatttga gtagattcat gtaaatccgg catttaataa actcctcacc
    10441 aattggcgat gatgatttat taattagacc atcaatgttc cacctcccgt cctgtgactt
    10501 cttaaagccg gtaaaggtat attgaatagg agtaagataa tatatcagtt caaatgaaga
    10561 ttcttttatc tgtttaaagt atataaaagt aatctgtaac aaacaagtta cgaaaactta
    10621 agaaataaca tccttatttt agatccagag gaaactttcg atcggaatct ctcccaaaat
    10681 gaacttccat ttcatgaaaa cgccgtaact actctccccc tataacacag ccacaccgga
    10741 tggactcaaa ggaagcgata aagaaattac caaaataata aaaacaaacc tcccgaataa
    10801 ctcgggaggt ttttaacaga gggtgcccac ctggagcttt ttttgaagat ctgggactcc
    10861 gccggtcaac ctagcagctt tacggataca agaaaaaacc ggcgggccga atcttcacat
    10921 ccattcgcgg tcccttcata aagcatggca gcgatgtggt cttttaagac ccggacactc
    10981 caacgttcat tgaggtacat ggtctcgtaa aaatcccttt taacagcgtc gtttcggggt
    11041 tttcaattga taaaactctc cggcttcatt caaccggtcc ggcttgaaaa acggatttgc
    11101 gatctttact ccccggataa tctggccgct gttcaagtct tcactcagta ttctttcgca
    11161 gcaagcgctt tcggcgctga caacaaccag agcgtcccag aaagaaatct ggtttagcac
    11221 actgcagtca accgcggatt cgatcaaatc cgggccaatc accacgacct caaagttttc
    11281 aaaagcacgg agaatgcttt tgacgagaat cggatccaag tttaattttt tagttgccgt
    11341 tacataaaac tcctgtaata cttgggtaga aataacaccc gtattggaat tttcctgcag
    11401 ttcctgaagc agaaaccgag attgcttttg tttcagcgga tcggctccat ccatggcata
    11461 aactaaaata ttggtatcaa tgaaaatttt agacatcgta taagtcctcg cgattccact
    11521 tttgcccccg ggagttggca ttcgcttgat ccattaaggc aaaacattcc tcgagccagt
    11581 tttgagagtt cggtaaaacc gtctgcgcca gcaattttcg gattaaagcg tttaatgatg
    11641 tattatgtcg tctggcatac tcccggcctt cttttaatag ttcatcatcc aaagaaatcg
    11701 tgatattttt catcgcatat caatgctcct tcgtcatcgc cagctcggca aatgaacaat
    11761 cccttaaaga cgactgccca aattatatat acctcactta caattacatt atacgtgcgc
    11821 acttattatg tgtcaagaat cgaggggtgc aggcaaaata tttgcatcca agtataaaaa
    11881 ctactactat tttaccgaag attcgcccca ctactgccag aagtattgca acgacttcat
    11941 gccgcttgtc gtggaagtcg aaacgaatga tttaaaagga ttaaaaccct aaaataattg
    12001 atcgctgctc gaattcacaa taccccgcag tgaccatgct ccctattgga cggcctattc
    12061 atttttcttc atatacagct ccttgatgat gttttcaatc ggcggttctc cgatggtcag
    12121 atcgttgata acgtaactct cgttgactgc ttgaataaaa cagttcagct cgatctttcc
    12181 catatccaac tctagctccg cattatactg tgagggttga ccggttaccg tcacaccggg
    12241 catggttgta aaatccggca gcggtttttg ggtcgaaagg ctgacgaact tttgatgacc
    12301 ggaaaatccg tgcaatgcct ccaaaccgtc gtcaaatacg atctcgccgt ggttcactac
    12361 aatcacccgg cgggccagac gttccacatc atcaaggtca tgagtggtaa ggataaaggt
    12421 tgtgccggtt cgattccgtt ccaggatgaa ctcgcggatc cggtccttgg cgatcacgtc
    12481 caatccgata gtgggttcat cgagaaagac gatcttggga ttatggagca tcgccatgat
    12541 aaattcgcac ttcatccgtt cccccaagga aagctgccgg gtaggtttac ggatgactcc
    12601 ctccactccg agcaggttga tcatctcttc cagccgtttc ttaaaatcgg aatccgcgat
    12661 ctcataaatc gctttgttca ttataaagga gtccagcggc ggaatgtccc agataagttg
    12721 cgacttttgc ccgaataccg ccccgatatt ggcaacatat ttgcgccgct gccgccaggg
    12781 tgtatatccc atgacatcca cctcaccggc agtcgggaac agaaccccgg taaggatttt
    12841 taaagtggta gattttccgg ccccgttggg tcccagaaac cccagaagtt caccttgttc
    12901 aacatcaaag gtgattcctt ttacggcatc aatcgcaatc ggttttcgaa ccaccaaact
    12961 ttttacggtt tccagaaatg aacttccccg ctcgtaggta agatatgtct ttcgtaaatc
    13021 cctgacttga atgattttca tcgcgaaatc ctcatcctcc cgcgctggtg tacttcgaaa
    13081 gcatccatcg ccaaaaggca aggctcaatc ctacgaacac cagacataca aacatcgtcg
    13141 caaatattcc gtcggtcggt tttccaagca acacggctgc agggaaaaaa ccaattgccg
    13201 ccaagggcag aatatacgag atgatcgcct gaaaccaccg ggagaagatc gaccgcgggt
    13261 aattcccgaa ggcggtggta ctatcgaaaa tctcataaac ccggctatta ccgacccatt
    13321 tgaatacact gcccgccatg aagagcgcaa atgaaaaaag taccattacc gagaagagga
    13381 aaagcaccaa aaactcgagc cactcccaca ttcccggagc cgtgagatta cgtaaggaaa
    13441 atcccatcac catcagcccg ccggcaatgg taccgatact atccggctca aaaccggtgg
    13501 ctactgccaa aaacagcacg gatctgggct tgatcaacaa caagtcataa gtgccctccc
    13561 tgacccgatc aagggtgttc cataccaccc caaagaataa aagacccgcc acacctcgcg
    13621 agagcataaa gattccctgc aacaaaagca tttcgtagat ggaccagccg ggaaaggaag
    13681 cgccggtccc atagatgagt aatgttaaaa acgggatcag cagttccgca accatgatca
    13741 tc
//
LOCUS       DASVMB010000094         6171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_234687,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000094 DASVMB010000000
VERSION     DASVMB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234687"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..996
                     /locus_tag="VF531_03140"
     CDS             58..996
                     /locus_tag="VF531_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HEX7712992.1"
                     /translation="MIIGIDTSCYTTSLALVTSEGNILLDSRSLLEVASGGRGLRQSE
                     ALFQHLQNLPLLLATDRWAPKQPPNLDAVCASVTPRPAPDSYLPVFRAGASFGQTLAG
                     LYGIPFISTSHQEGHIRAGLAGNPELKGDFLAWHISGGTTELLLVRPDHPGFTIQKVG
                     GSSDLHVGQFIDRIGVALGTGFPAGPELERMAERTNPEIHLPVAVDGLSLSFSGPASA
                     AERALRSGTGASLAYAVLECIGRSLFMVTRRAAEIYGVNQVLAVGGVSANQMIRTYLT
                     DAGTREGLNFYFGSRELSSDNAVGAALIGRDFLSGN"
     gene            1154..3856
                     /gene="polA"
                     /locus_tag="VF531_03145"
     CDS             1154..3856
                     /gene="polA"
                     /locus_tag="VF531_03145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7712993.1"
                     /translation="MGKRLMIIDGYSLANRAFFALPPLTTSNGQPTNAVYGMAMMLLK
                     LLEEAKPDYFLAAFDVAAPTFRHQEYTAYKGQRLKMEDSLKSQIPLIRRLLEIMRIPV
                     YEMPGFEADDLIGTFAKKASSQGLKVEIVTGDRDSFQLVDPGIKVLYTRKGITELDRV
                     DEAYIQERYQLRPEQLVDLKGLMGDASDNIPGVPGVGEKTALRYLHEYSSLEAVYDHL
                     DEISRAHDRELLGKYKEQAFLSRHLARIVTNLDLPLDLPMCCRHLDYDSQELLRFCQE
                     NEFNSLVRKLSGNAAPVETRLREIEFQVKSIDETSLTPVLTRIEQQKSCYLQFCTAAA
                     NWVQIKPLGLGLGTAEPGENWFYPMDSGGTIPESLRRMLADPGVVKIGHDLKKQMQIA
                     AGLGITLAGPFEDTMIAGYLISAGSGDLELEALCQTYLGLSIPGWRNERSKLFPVFNL
                     PEDIKSDALARITGGRIAGIRGLLSVFQEQLKEETSETASLKSLYYDVELPLVKVLFS
                     MERTGVKVDPESLRGYGKTLKLREKELEVEIYRLAGEEFNINSPKQLGALLFEKLGLK
                     APKKTKTGYSTDAEVLDSLAEAHPIIPQILEYRQNQKLQSTYIDSLITLIHPRDGRVH
                     TTFNQAVTTTGRLSSTEPNLQNIPIRSEEGRMIRRAFVAEEGNCLLSADYSQIELRIM
                     AHFSQDPAFREAFIKGDDIHRFTAAAVYGVPVTEVTREMRDKAKAVNFGIIYGISGFG
                     LAKNIGVSRKEAEIFIETYFSRYPGVQDYVNQLIETAKATGEARTLLGRIRKLPDLYS
                     RNFTLRSFAERTARNTPIQGTAADIIKLAMVRIEERLAAEPELGKLLLQVHDELVFEV
                     SEKNWRELAAIVQPAMEHAVDLSVPLTVAFKKGTDWCEMVPVEF"
     gene            4081..4899
                     /gene="mutM"
                     /locus_tag="VF531_03150"
     CDS             4081..4899
                     /gene="mutM"
                     /locus_tag="VF531_03150"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX7712994.1"
                     /translation="MPELPEVETIRRTLEPKLVGRTIRGGAVYHPKLIQGQSEPDFFA
                     CIRNRNILRITRRGKYLLIHLSGERIIGIHLRMTGQLAVVPQDEPEAPATYLRLQLDN
                     GTELRFCDQRKFGRIFCSPEDSLPGGLTKLGPEPLDPDFTADELKKRLARRHLAIKKA
                     LLNQEILAGMGNIYADEALFTAGIHPERPADSLSGSELKNLFHAIRRVLEEGIQNRGT
                     TKRNYRDGDGNPGSNQEHLQVYDREDQACLKCGTTIIKTFVGGRGTHFCPKCQK"
     gene            4929..5546
                     /gene="coaE"
                     /locus_tag="VF531_03155"
     CDS             4929..5546
                     /gene="coaE"
                     /locus_tag="VF531_03155"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7712995.1"
                     /translation="MQSIGLTGGIATGKSSVADLLKRMDVPVLNADEVAHRALETGTD
                     TFMLVIQSFGKDILDFNGEIDRRKLGRIVFNNPEARHRLEAIIHPTVIREIQTRLKQA
                     ADGCIKLMAVEVPLLFEAGMEDQFDYIWVVSAPTHAQLKRLQERDGLSKVEAQARVDS
                     QWPLALKEAKADAVIQNSGNLQDLEIQVSKLLQNLGWTVGAGNRN"
     gene            5803..>6171
                     /locus_tag="VF531_03160"
     CDS             5803..>6171
                     /locus_tag="VF531_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7712996.1"
                     /translation="MKQRTFTYKKLVILTAFLVVLWHKPILRLFFVMEYGNQIVAYSR
                     ENQVNPAMISAIVFTESRFDTLAKSHKGALGLMQIMPETGAGVARELNWQDFSSKDLL
                     DPDKNLRVGIRYISYLKRQFN"
BASE COUNT         1525 a         1443 c         1696 g         1507 t
ORIGIN      
        1 cctttttttt catgggcctt tttgtgaaaa aacggcaaaa accggattca ttgcagaatg
       61 attattggaa ttgatacgag ctgttatacc acttctctag ccctcgtcac gtctgagggc
      121 aatattctcc tggacagccg cagcctgctg gaagtggctt ccggcgggcg gggacttcgt
      181 caatccgagg ctttgtttca gcatctgcaa aatctcccgc ttctcttggc aacggatcgt
      241 tgggcaccca agcaacctcc aaatttggat gcggtctgcg cttcagttac accgcgcccg
      301 gcgcccgatt cttatctccc ggtttttagg gccggagcct catttggcca gactctggcc
      361 ggtctttacg gtatcccatt cataagcacc agtcatcagg aaggccatat tcgtgccggt
      421 ttagcgggaa atccggagct taaaggggat tttttggcct ggcatatctc gggcggaacc
      481 acggagttgc tcctcgttcg gcccgaccac ccgggattca ccattcagaa ggtcggcggt
      541 tccagtgatc ttcatgtggg gcagtttatc gaccggatcg gggtggcttt ggggacgggt
      601 tttccggcag gcccggagtt agaacgcatg gcggagcgga ctaatccgga aattcatctc
      661 ccggttgcgg tggatggttt aagtttaagc ttttcaggtc cggctagcgc cgcggaacgt
      721 gcgcttcgtt ccggcacagg ggcgagtttg gcctatgcgg ttttggaatg tatcggcagg
      781 agtttattta tggttacccg gcgtgccgca gaaatttacg gggttaatca ggtattggct
      841 gttggaggcg tttcagccaa tcagatgatc cggacttact taacagacgc cggaacccgt
      901 gagggactga acttttactt tggttcccgg gaactctcca gcgacaacgc ggtgggagcg
      961 gcgctgatcg gccgcgattt tttatccggc aactaatacg atgtcgcttt ctgtcgcttt
     1021 taatggaata agcatgcgct ataaataaaa ggcacttgta aacagaccga tttgcatccg
     1081 attgttcgat gttgattgca aattggtgaa aaatagggtt tgggacgttt gaaaaagcaa
     1141 gggaggggct attttgggga agagactgat gatcatcgac gggtatagcc tggccaaccg
     1201 ggcgtttttt gcgttgccgc cgcttaccac cagtaatggt cagcccacaa atgcggttta
     1261 cggaatggcg atgatgcttt taaagctttt ggaagaagcc aaaccggatt attttttggc
     1321 cgcttttgat gttgcggccc cgacattcag gcaccaggag tacacggcgt ataaagggca
     1381 gcgactcaag atggaagatt cgctcaagtc acaaattccc ctcattcgtc ggctgcttga
     1441 gatcatgcgg atccctgttt atgaaatgcc cggttttgag gcggacgatc tgatcggaac
     1501 cttcgccaaa aaggcctcgt cccaagggtt gaaagtggag attgtcacag gggaccggga
     1561 ttcctttcaa ctggtagatc ccggaatcaa agtgctctat acccggaagg ggattaccga
     1621 gttggatcgg gtagatgaag cctatattca agaacggtac cagcttcgac cggagcaact
     1681 ggtcgacttg aaaggtctaa tgggggatgc ctcggataat attccgggag ttccgggtgt
     1741 cggtgaaaag actgcccttc gttatttgca tgaatattct tccctggagg ccgtttatga
     1801 tcacctggat gagatttccc gggcgcacga ccgggaattg ctcgggaagt acaaggaaca
     1861 ggcatttctc agtcgccatc tggcacggat tgtgaccaat ctcgacttgc cgctggattt
     1921 accaatgtgt tgccggcatc tggattatga ttcccaggaa ttactccgtt tctgtcagga
     1981 gaacgagttt aattcattgg tcaggaaact ttccggaaac gctgcaccgg tggaaacacg
     2041 gctccgggag attgaatttc aagttaaatc catagatgag acaagcttga ctccggttct
     2101 cacccggatc gaacagcaaa aatcctgcta tcttcagttc tgtaccgcgg cagccaactg
     2161 ggtacagatt aaaccattgg gtttaggcct tggaacggca gaacccggag agaactggtt
     2221 ctacccgatg gactccggcg gcaccattcc ggaatcactc cggaggatgc tggctgatcc
     2281 cggggtggtt aagattggcc atgacctaaa aaagcagatg cagattgcag ccggattagg
     2341 aatcacactg gcaggcccct ttgaagacac catgattgca ggatatttaa tcagcgccgg
     2401 tagcggtgat ttggaactgg aggcactctg ccaaacctat ctcggacttt caatccccgg
     2461 atggcgaaat gagcggagta agcttttccc agtgttcaat ctgccggaag atataaagtc
     2521 ggatgccttg gcccggatca ccggcggccg aatcgccgga atcagagggt tactatccgt
     2581 ctttcaggag cagcttaagg aagaaacgtc tgaaacggca tcattaaaat cgctttatta
     2641 tgatgttgag ctgccgctgg taaaagtatt attcagcatg gaacgaaccg gagtgaaggt
     2701 cgatcccgaa agtttgcgcg gttacggaaa aacactcaag cttcgggaga aggaactgga
     2761 agttgaaatt taccgtctgg ctggtgagga gttcaatatc aactccccca aacaattagg
     2821 ggcgctgctc tttgagaagt tgggattgaa agctccgaaa aaaacgaaaa ccggttattc
     2881 cacggatgcg gaggttttgg attctttagc cgaggcgcat ccgatcattc cccaaatcct
     2941 ggagtaccgg cagaatcaga agctgcagtc cacgtatatt gattcgctga tcactttgat
     3001 tcatccccgg gatggccgtg tacacaccac attcaaccag gcagtaacca ccaccgggag
     3061 attatcaagc actgaaccca atttgcagaa catcccgatc cgttccgagg aaggccggat
     3121 gatccggcgc gctttcgtgg cagaggaagg aaattgtctg ctttcggcgg attattccca
     3181 gattgagctg cggattatgg ctcatttctc tcaggatccc gctttccggg aggcgttcat
     3241 caaaggagac gatattcatc gtttcaccgc cgccgcggtt tacggtgttc ccgttaccga
     3301 ggttaccagg gagatgcgtg ataaggccaa agcggtcaat tttgggatca tttatggaat
     3361 cagcggtttc gggctggcta aaaacatcgg tgtcagccgg aaagaggcgg agatatttat
     3421 tgagacctat ttctcccgtt atcccggagt tcaggactat gtcaaccaat tgatcgaaac
     3481 cgccaaggct acgggggaag cccggaccct attgggacgg atccggaagt tacccgattt
     3541 atactcaaga aactttaccc tgcgctcatt tgctgagcgt actgcgcgga atactccgat
     3601 ccaaggcaca gccgcggata tcattaaatt ggcgatggta cggatcgagg agcggttagc
     3661 tgcggaaccg gagttaggaa agttactact ccaggtccac gatgaattgg tttttgaggt
     3721 atccgaaaag aactggcgcg aacttgccgc cattgttcag cctgccatgg aacatgccgt
     3781 tgacctatcg gttccactca cagtggcatt caagaagggg actgattggt gcgagatggt
     3841 cccggttgag ttttaaaggc atgagacaag aggcgagagg cgtgaggcac gggtaactca
     3901 ccccgcgtca gcctggagct atcagtataa ccctacgccg cgttttaacg cgacctctct
     3961 ttctgaaaag agaggggacg ggggtgagtt gatttggact agagtaaaac ttataattac
     4021 tcacgcctca tgccttaatt aactttggac tttcgacttt taatctagga gaaaacaatt
     4081 atgccggaac tcccggaagt agaaacgatc cgccgtaccc tcgaacccaa actggtcgga
     4141 cgaaccattc ggggtggtgc ggtttatcac cccaagctca tccagggtca gtccgaaccg
     4201 gattttttcg cctgtatccg gaatcggaat attttgcgaa ttacccggcg gggtaaatat
     4261 ttgttaattc atctttccgg ggaacgcatc attggaatac acctacggat gaccggccaa
     4321 ttggccgtgg tacctcaaga tgaaccggag gcaccggcaa cctatctccg cctgcaactg
     4381 gataacggaa cggagctgag gttctgcgat caacggaagt tcggccggat attttgcagc
     4441 cctgaggatt cattacccgg aggccttaca aaactcggac cggaaccgct ggatccggac
     4501 ttcacagcgg atgagcttaa gaaacgactg gcccggcggc atctggccat aaagaaggcc
     4561 ttattaaatc aagagatact ggcgggaatg gggaatatat acgcggatga ggctttattc
     4621 acagcgggga ttcatcccga gcggccggcc gattccttaa gcggttcgga gctcaagaac
     4681 ttgtttcatg ccatccgccg ggtattggag gagggaatcc aaaaccgcgg tactaccaag
     4741 cggaattatc gcgacggcga tgggaatccc ggctccaacc aggagcactt acaggtttat
     4801 gatagggagg atcaagcctg cctcaagtgc ggtaccacca tcatcaaaac attcgtgggc
     4861 ggtagaggaa cccatttttg tcctaaatgc cagaagtagt tgagttttga cctttcgagg
     4921 gggtttttat gcaaagcatt ggtttaaccg gcggaatcgc cacgggtaaa tcgagtgtgg
     4981 cggatctttt gaaacggatg gatgtcccgg ttttgaatgc ggatgaagtt gcccaccggg
     5041 ccctggaaac cggtaccgat actttcatgt tggtaatcca gagtttcggt aaggatattc
     5101 tggatttcaa tggtgaaatc gaccggagaa aattgggccg gatagttttt aataatcccg
     5161 aagctcgtca tagactggaa gcgatcattc acccgacggt gatccgggag attcaaacaa
     5221 ggctaaagca ggcggcggat gggtgtatta agctgatggc cgtggaagtc ccgttacttt
     5281 ttgaagccgg gatggaagat caatttgact atatttgggt agtaagcgcc ccgacccatg
     5341 cgcaactaaa acgtttgcag gaacgagacg gcttaagcaa agtcgaggca caggcacggg
     5401 tcgattccca atggccactt gcgctcaagg aggcaaaggc cgacgcggtg atccaaaact
     5461 caggcaactt gcaggatctt gagattcaag tgtctaagtt acttcagaac ttgggatgga
     5521 cagtaggagc aggtaacagg aactaggcaa caggtaacag gcaataggca acaggtacga
     5581 tgcaactcac cctgcgttaa ccttatactt tcagtataac cctgcaccgc gttttaacgt
     5641 gacctctctt tctgaaaagg gaggggtcgg gggtgagttg ctaatagtca tcgcgccctg
     5701 tggggcacgc ggtaacaatg agaggcaaac ttctccaaat ggttaattca ttcccctttt
     5761 gcctagtgcc tcttgtctct tgcctattca aggagagttg atttgaaaca gcgtacgttc
     5821 acctataaga agctggtgat cctgaccgcc tttttggtgg ttttatggca taagccaatc
     5881 ttacgccttt tttttgtgat ggaatacggg aatcagatcg ttgcttacag tagggagaac
     5941 caggttaatc cggcgatgat cagcgccatt gtctttacgg aaagtcgttt tgacaccttg
     6001 gccaagtccc ataaaggagc tttggggttg atgcaaatca tgcccgaaac aggggccgga
     6061 gttgcaagag aattgaattg gcaggatttt tcatcaaagg acttgctgga tccggataaa
     6121 aacctaaggg tgggaatccg atatatctct tatttaaaac gccagttcaa c
//
LOCUS       DASVMB010000095         4479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_528307,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000095 DASVMB010000000
VERSION     DASVMB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528307"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1073
                     /locus_tag="VF531_03165"
     CDS             222..1073
                     /locus_tag="VF531_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7712997.1"
                     /translation="MTDGRIDYQDPSRTLIISRHKVTGHYDMAEEHFHNQHELYYLYS
                     GERNYFIKDRTFLVGQGNLVLIPAFELHKTTDTGLPNHERMLIKFSEGFLQTARDPEI
                     GTILTALGKKAPVFRLSFEEQQLVEGLFLQMLREAKERRPGCETLLRALLMQILIYVY
                     RSREQEGIDPSAYLSPTHKKVSAIVRYINQHYAEDLTLTALAKRFFVSPYYLSRNFKK
                     VTGFNLVDYLNHVRVKEAERYLRETRLKVIQIAGETGFGSIAQFGRVFKSITQMTPLE
                     YRKRTLG"
     gene            1330..1500
                     /locus_tag="VF531_03170"
     CDS             1330..1500
                     /locus_tag="VF531_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HEX7712998.1"
                     /translation="MSRDSEIQLAIYRDVLKKSVKRYGLSSPRVLRISRRVDRFIVAL
                     LRESTGENNQKK"
     gene            1526..2935
                     /locus_tag="VF531_03175"
     CDS             1526..2935
                     /locus_tag="VF531_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7712999.1"
                     /translation="MLQRVLCLALLLFGILGSGLTVSAEPDTLTLDQAVQMALKADYQ
                     IKAAMNDLEVAKLAVRNEAVKIFPKADIGGQYRYQAANDTYPNSFQIVIQETIPTTWN
                     LYGQKIVSGIAAAGWDQVSAEAALQIKQAEIICNTCEDYCNVLKAQQVLKLQTEALEK
                     QRKASALAIQQLNLGKITKPDQLKIENFLNQAEFNLEKDRSELEIARQKLANQIGLED
                     LVNLDLAETTLSPDAMDLKLDALQEKALKRRLELQKAEINIKKAQRVWAQARNAELPT
                     VSLGYNSQSETRSLGLNYEFTNGDLSWFAAEQSGSDQSQADVLSGTDNSQGYYGNNQN
                     YFTLKLSWSVDFGSAANDTNQSRYNLDNARLDLEKERQAILLEVKQALADYQLAVKEY
                     ETNQKALPYYEKDLEIKRLQEQLGAITCIDLATAEQDAASARITAVRSGWDRYLTLQK
                     LKKAVGDLYPFDQLEEKHS"
     gene            2932..4131
                     /locus_tag="VF531_03180"
     CDS             2932..4131
                     /locus_tag="VF531_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7713000.1"
                     /translation="MKKSMARASITAMILLIVLAAGWLVVKEHLQTAKAETTGKYDLT
                     SVRRMDLSDRINATGNVVLEKNASIYPPYNATVKKILVKPGDPVHKGDVMMILQLDDA
                     EMVNYSSNWKSSLEQAQDNLTVAKKALERETVLYKAQGTTIDAMENAQSKVRQYQEQV
                     NEYRLKLESLTKNGVDNDNNVLVRAPFDASISWIDVKLEEAVTTATQLLTLCGDSAIR
                     VEVNVDQGDIGLMKMGLEASISANDQNRTTIQGIVDSFGSTGTTSSGVVTFPVVIKPK
                     TPGKLNNLLKSGMTVDVTIMVDSHPNVLAVPSRAVREENGQTVVQVLNQGRLVTKKVQ
                     LGFQSSDQVEVLSGLAENEQVAVPVIQAASQSGTGESGKSQSNSGMMGGPMGGPMGGP
                     MGGPVGR"
     gene            4137..>4479
                     /locus_tag="VF531_03185"
     CDS             4137..>4479
                     /locus_tag="VF531_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7713001.1"
                     /translation="MIAIENLAKIYQMGDTEVRALDKVSLSVDEGAMVAIMGPSGSGK
                     STFMNIIGCLDQPSAGSYRLNGSEVAKLNEVEQARLRIREIGFVFQSFNLLSNLTALQ
                     NVELPLIYAGLG"
BASE COUNT         1274 a          997 c         1179 g         1029 t
ORIGIN      
        1 tcatgcctcg cgcctgttgc ctaattgact ctacttatga cttcggatat tcgacaatga
       61 actatttcca ttatatctgg acaacccgga aattgctgcg cggtttttgt caaattgaga
      121 tatagatttg catgttttgc tttaaaggaa ccggcagcgc tttccggatg tgattctttc
      181 gcgaatcaat gggatatcaa ctattcggaa caggtgattt gatgacggac ggtcgtatcg
      241 attatcagga tccatcgcga accttgatta tatcccggca taaggtaacc ggtcattacg
      301 atatggctga ggagcatttt cacaatcagc acgaactcta ttatttatac tccggagaac
      361 ggaactactt catcaaagac cgaacttttc tggtcggaca gggaaatttg gtcctgattc
      421 ccgcgtttga gctccacaag accaccgata ccggattgcc caatcatgaa aggatgctca
      481 tcaaatttag cgagggcttt cttcaaaccg caagagatcc ggagatcggg acaatactca
      541 ccgcgcttgg taagaaagct ccggtattcc ggttgagttt cgaggagcaa cagttggtgg
      601 agggactctt ccttcagatg ttgcgggagg ccaaggagcg ccggcccggt tgtgaaacgt
      661 tactgcgcgc gctattgatg cagatattga tctacgtata ccgcagccgg gaacaggagg
      721 gaatcgaccc cagtgcgtac ctgagtccga ctcataaaaa agtctcggcc atcgtccgct
      781 atatcaatca acattatgcg gaagacttga cccttaccgc gctcgccaaa cgtttttttg
      841 tcagccctta ttaccttagc cgtaatttca agaaagtcac cggtttcaac ttggtcgact
      901 atttgaatca tgtccgggtc aaagaggccg aaaggtatct ccgtgaaacc aggcttaagg
      961 tgattcaaat cgccggggaa acgggattcg ggagtattgc ccaattcggc agggtgttta
     1021 agtcgatcac ccaaatgaca ccgctcgaat atcgaaaacg gacacttggg tagatggtag
     1081 cttgaaacga tatggtgaat catagactac gtatagatca gggtattttc ctggtctttt
     1141 tttatgacca actttctccg gcgttcaaga gacttccata ggttgagaat caacaacgga
     1201 acacacgaaa tttcatgaaa aaaattccaa agtatcccgg taacaacacg gaatgttcac
     1261 agaatgaaca catgggcgat ttagaataaa tatcaagaaa cgggaagaac aggcgggaag
     1321 gaggctcata tgagcaggga cagtgagatt caattggcta tttatcgaga tgtgctgaaa
     1381 aaaagtgtaa aacgttacgg attatcttcc ccccgtgttt tacgaatcag ccgccgtgtc
     1441 gatcggttca tcgttgcatt gctccgggaa agcaccggag aaaataacca gaaaaagtag
     1501 gggatttact ttggagagaa agcccatgtt gcagagggtg ttatgccttg ccttactgct
     1561 gttcggcata cttggatccg gattaacggt atccgctgaa ccggatacct taaccctgga
     1621 tcaagcggtg cagatggcat tgaaagccga ttatcagatc aaggccgcca tgaatgattt
     1681 ggaagtagcc aaattggccg ttaggaacga ggccgtgaag atcttcccca aagcggatat
     1741 cgggggccaa tatcggtatc aagcggcgaa tgatacctat cccaactcct ttcagatagt
     1801 catccaggag acaattccaa ccacctggaa tctatacggt caaaaaatcg tctccggtat
     1861 cgccgcggcc ggttgggatc aagtaagcgc tgaggctgcg ctccaaatca agcaggctga
     1921 aatcatttgt aatacctgcg aggattattg taacgtctta aaagcgcagc aagttttgaa
     1981 actccaaaca gaagcgctgg agaagcagag gaaagctagc gctctggcca tccaacaatt
     2041 aaacttaggc aagattacca aacctgatca actgaagatc gaaaattttt taaatcaagc
     2101 cgagttcaat ctggagaaag accgttccga actggaaatt gcccgccaaa aacttgccaa
     2161 tcaaattggg ctagaggatc tcgtgaatct agatttggcg gagacgacac tctcaccgga
     2221 tgctatggat ctaaaactcg acgcgttaca ggaaaaggcg ctaaaacgtc ggctggagtt
     2281 gcaaaaggca gagatcaata tcaaaaaagc ccaacgggtt tgggcgcaag ccagaaacgc
     2341 cgaattgcct acggtctcct tgggctacaa cagccaaagt gagactcgca gtttggggtt
     2401 gaactatgaa tttacaaacg gtgatctttc ctggtttgcg gccgaacagt ccgggtccga
     2461 tcaatcccag gcggatgtac tctcaggtac ggataacagt cagggttatt atgggaacaa
     2521 tcaaaattat tttaccttga aattgagctg gagtgtagat tttggcagcg ccgcgaatga
     2581 cacgaatcaa tcccgataca acttggacaa tgccaggctg gatctggaaa aggagcgtca
     2641 ggcaatcttg ctcgaagtaa aacaagccct ggcagattat cagttggctg tcaaagagta
     2701 tgaaacgaat caaaaggcat tgccttatta tgaaaaagat ctggagatta aacggctaca
     2761 ggagcaactg ggcgcaatca catgtatcga cctggctacg gcggagcagg acgcggcatc
     2821 ggcccggatc actgccgtta gatcgggatg ggacagatac ctgacgctac agaaactgaa
     2881 aaaggcagtc ggagatttat acccattcga tcaattggag gagaaacatt catgaaaaag
     2941 agtatggcgc gcgcgagcat cacggcgatg attttattga ttgttctagc cgcaggttgg
     3001 ttggtggtaa aggagcatct gcaaacagcg aaggctgaaa ccacgggtaa gtatgatctc
     3061 acatcggtac gacgcatgga cttgagcgac cggatcaacg ccaccggcaa tgtggtgtta
     3121 gagaaaaacg cttccattta cccgccttat aacgcgacag tcaaaaagat tctggtcaaa
     3181 cccggcgatc cggtacataa aggcgatgta atgatgattt tgcaactgga cgatgcggaa
     3241 atggtaaatt actcatcgaa ctggaaatct tcactggaac aggcacagga caatctgacc
     3301 gtggcgaaaa aagcattgga acgcgagacg gttctttata aagcccaggg gacaactatc
     3361 gatgcgatgg aaaatgctca aagcaaagtc cggcaatacc aggaacaagt gaatgagtat
     3421 cgattgaaac tggaatccct gaccaagaac ggggtggata atgataataa tgttttagtc
     3481 cgggctccgt ttgacgccag tatctcctgg attgatgtaa agctggagga ggcggttaca
     3541 accgcgaccc agctcttaac cttatgcggc gatagcgcca tccgggtcga agtcaatgtc
     3601 gaccagggtg atattggttt gatgaagatg ggtcttgagg cttcgatcag tgccaacgat
     3661 caaaaccgca ccacgattca agggatagtt gattcgttcg gcagcaccgg aaccaccagt
     3721 tccggagtag taaccttccc ggtggtgatt aagccgaaga ctccgggtaa attgaataat
     3781 cttttgaaat ccggaatgac ggtggatgta acgattatgg tagattctca cccgaatgtg
     3841 ctggccgtac cctcgcgggc ggtacgagag gaaaacgggc agaccgtagt acaagtcctg
     3901 aatcagggac gattagttac caaaaaagtt caattgggat tccagagttc cgatcaggtg
     3961 gaagttttat cgggtctggc cgagaatgaa caggtcgcag taccggtcat tcaggcggcc
     4021 agtcaaagcg gaactggcga atccggtaaa agccagtcaa atagcggcat gatgggcgga
     4081 cccatgggcg gtccgatggg tgggccaatg ggcggtccgg taggcaggtg agcgggatga
     4141 tcgcgattga aaatcttgcg aagatctatc aaatgggaga taccgaagtc cgggccttgg
     4201 acaaggtttc attgagcgtg gatgaaggcg caatggttgc catcatgggc ccgtccggct
     4261 ccggaaaatc gacgttcatg aatatcatcg gctgcctgga tcagcccagc gccggctcat
     4321 accggttgaa cggcagcgaa gtggccaagc tgaacgaggt tgaacaagcg agactcagga
     4381 tccgggagat tggcttcgtc tttcagtctt ttaatctgct ttccaacctg accgcccttc
     4441 aaaacgtgga actgccgctg atctatgcgg gtctgggag
//
LOCUS       DASVMB010000096         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_675741,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000096 DASVMB010000000
VERSION     DASVMB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675741"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF531_03190"
     CDS             <1..465
                     /locus_tag="VF531_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX7713002.1"
                     /translation="KLSATIFGAAGSVSLLFLLEILGTIGVFGATFLQRFAWSNLALV
                     QRINIPYLVLEQIGPLFLVVWLTMFLCSMAFNFYLVAGGVQQMFPRFNYRLLVLILLA
                     VVGAAGLLPPNMSLVTRIFSVYRVWVMAPLIGYPLIVYGMALIRGITGKCEV"
     gene            549..1685
                     /locus_tag="VF531_03195"
     CDS             549..1685
                     /locus_tag="VF531_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7713003.1"
                     /translation="MKLIFRLIIISLIPLMLAGCWDFEEVDRRSFATTIGIDALPRDK
                     VMISVQIPLPQKMLPPGIKGAEEGKVFSTISLAGSTVGDAFNNMQTKAYRDLVIQQNK
                     SIIIGADAARRGVEPLLEFLARNPKAPPQALIFVTKNYTARDILTFAPVERTLPGLMF
                     GQSAQAIVKYNRTFFIPIWQFEQKLVHETKDPYAPLISVDQAEGIFIEEGLAVFNKDR
                     LTGELTGEEAQMAGIITGFMKKGTLTVPIPRAHARDSKIAFRNLSSKSRIKVLLKNNR
                     PFFKLKIDLYGQMDELAGWGHRETTPADIRNLERNVKRVINPKISAVIHKLQGFNSDI
                     LDFGEQLRVQHLQVWNRIDWKKVYSTVDFQVDLRVHIWGNGVLY"
     gene            1891..2685
                     /locus_tag="VF531_03200"
     CDS             1891..2685
                     /locus_tag="VF531_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7713004.1"
                     /translation="MLGHYLFILFPAGILAGITSSAAGLASLISYPALLAVGIPPVYA
                     NVTNTAALIFTGVGSGVSSRRELRGHGRELFKLLPLTVGGSLCGSMLLLVAPAATFEH
                     IVPFFILGAALLILRPKQAALDQQRPLVAASAETGDRELRSKAAVAAYSIAIFLVGAY
                     TGYFGAAGGVVMLAILSATSHAEFAEYNALKNVSLGAANLVATLIYIFRSHIDWLAAA
                     PLAAGFFIGGTIGPSIVRHVSSKVLRVVIAAGAMGLAIALFIQTYF"
BASE COUNT          681 a          629 c          680 g          806 t
ORIGIN      
        1 aaattatccg ccaccatctt cggagccgcc ggcagcgttt cgctgctctt tttgttggag
       61 atcctgggga ccatcggggt attcggagca accttcctcc agcggttcgc ttggtctaat
      121 ttagctttag ttcagcggat caatatccct tatctcgttt tagagcagat cggtccgctg
      181 tttttggtag tatggttaac tatgtttcta tgtagcatgg cttttaattt ttatctggtg
      241 gctggaggag tccagcagat gtttccgcgt ttcaattatc gccttctggt tttaatcctg
      301 ttagctgtgg ttggcgcggc tggtttattg ccgcccaata tgtctttggt cacgcgcata
      361 ttttcagttt accgggtttg ggtgatggcg ccgctcattg gctatccatt aatcgtctat
      421 gggatggctc tgatccgggg tataacagga aagtgtgaag tgtgaaaata gatgataaat
      481 tacaggaata gggtcaaaga cctatattcc caatgaattt taatctctct tacaagagga
      541 aaactgccgt gaagcttata tttcgtttga taatcattag tttaatacca ctaatgcttg
      601 caggttgttg ggactttgag gaggttgacc ggcgctcctt cgccaccact attggaattg
      661 atgccttacc ccgggacaaa gtgatgatct ccgtccaaat tcccttgccg caaaaaatgc
      721 tcccaccggg catcaaaggc gcagaggaag gaaaagtatt cagtaccatc agtttagccg
      781 gatcaacggt cggcgatgct tttaataata tgcagaccaa agcctaccgg gatttagtca
      841 ttcaacaaaa caaatccatc atcatcggag cagatgccgc ccggcgcggg gtcgaacctt
      901 tactggaatt tttagcccgc aaccccaaag cgccgccgca agcattgatt tttgtcacca
      961 aaaattatac cgccagggat attctgactt tcgctccagt ggagcggact ttaccaggat
     1021 tgatgttcgg ccagtcggct caggcaattg tgaaatataa ccggactttt tttatcccca
     1081 tctggcagtt tgagcaaaag ctggtccatg agacgaaaga tccctatgct cctttaatca
     1141 gtgttgacca agctgaaggt attttcattg aagaaggctt ggcggtgttt aataaagatc
     1201 gtcttaccgg agagttaacc ggcgaggaag cacaaatggc cggaatcatc accgggttca
     1261 tgaaaaaggg gactctaacc gtcccgatcc cccgcgcgca cgcgcgcgat agcaaaatcg
     1321 cttttcgcaa tttgtcgagt aagtcgcgga tcaaagtatt attaaaaaat aaccggcctt
     1381 tttttaaatt aaaaattgat ctttacggac aaatggatga actggcgggc tggggtcacc
     1441 gggagaccac tccggcggat attcggaatt tggaacgaaa cgttaaaaga gtgatcaatc
     1501 caaaaatcag cgcagtcatc cacaaattgc agggctttaa ttcggatatt ctcgattttg
     1561 gggaacagtt acgagtgcaa catcttcagg tttggaaccg gatcgattgg aaaaaagtct
     1621 attcgacagt tgattttcaa gtggatttgc gggtccatat ttgggggaat ggggtgttat
     1681 attagattag ttcatccgga cgctcctata ttcggggtcg gaatcgatga tttcttcaac
     1741 gatgatcaac ttgtatttac aaaatagtac gatataataa gtactattac tggggaaaaa
     1801 agaatttctg cttttccttg taggctacgc aatcgggtca gcatagccgt atcatttttg
     1861 acaaactaat ttggaaagta aagagcaatc atgttagggc actatttatt cattcttttt
     1921 ccggctggaa tcttggctgg gattaccagt tcggctgcag gactggcctc cctcatttcc
     1981 tatccggcat tgcttgccgt aggcatacct ccagtttatg ccaatgtcac aaacactgcc
     2041 gcacttatat ttacaggagt ggggtccgga gtatcttcaa gacgcgaatt acgcggccat
     2101 ggccgcgaac ttttcaaact gctgccgctt acagtaggcg gaagtctctg tggaagtatg
     2161 cttttattgg tggccccggc ggcaacattt gaacatattg ttcctttctt tatcttgggc
     2221 gcagctttgc tgattttgcg tcccaagcag gctgctcttg atcaacagcg accgttggtt
     2281 gccgcgagcg ctgaaactgg agaccgtgag cttcgcagca aagcagctgt tgccgcatat
     2341 agtatagcca tctttctcgt cggggcttat acagggtatt ttggcgcggc tggcggcgtt
     2401 gtgatgctgg caattctctc ggcgacaagc catgcggaat ttgctgagta taacgcgctg
     2461 aaaaacgtat cattaggcgc tgcaaacctg gtcgccactc tgatctatat ctttcgctcc
     2521 catatcgatt ggctggcggc ggcgccgttg gccgccggat ttttcattgg cggaaccatt
     2581 ggcccgtcga ttgtgcgcca tgtttcttcc aaagtgctgc gcgtggtcat tgccgccggc
     2641 gcgatgggac tagccattgc tttattcata cagacttact tttagtaaaa cattccgatg
     2701 caaaatgcat gttaggaagc acttcttaca aaagatattt cattatgagt tgtcctttac
     2761 tatccctgta aacctcggtg aatccaagtt ctctat
//
LOCUS       DASVMB010000097         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_528525,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000097 DASVMB010000000
VERSION     DASVMB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528525"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VF531_03205"
     CDS             complement(<1..758)
                     /locus_tag="VF531_03205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7713005.1"
                     /translation="MNLKRFRLLMGITFLLCFGVTCRGADRPVSTGMVPVEEQVVTLT
                     AVGDVVMHTPIVDSAFDPATGAYDFRPIFTGINQDLLRSDLAVGVLESPLAGPEEKYT
                     GYPRFNAPGAIADALKWAGLDLVFTAHNHCLDRGISGLESTLAYLDQIGLPYVGGQRQ
                     PKQARYRIVAVKGIRLAFLSYTTITNGIATPSGWEWAVNRLDFSRVAEDIAAAKRNGA
                     DGIIMAMHTGTEYQRQPSPDQRKLIDRLLDMGVD"
     gene            complement(1258..2058)
                     /locus_tag="VF531_03210"
     CDS             complement(1258..2058)
                     /locus_tag="VF531_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713006.1"
                     /translation="MKGLLRAYVPKVLGIWLLIIICAVTPGAYGAQNDLGTSIANLAQ
                     LVTRQFGVTEARVAAVQGDTVYLNAGSTKYIKEGTLFEIVAEGAPVTDPTTRARIGTI
                     ETHVADVKIISVRTNLCIGQVINRGQSTVATVPLAVGQKAVEKARKFSIAVVPFEYLN
                     SKDGITARVAQELMINELIKTGRFVVADSLRTEQIVKMLSATSTPGSVKFTKEAGKLL
                     GVSYVMYGYLTDLPGFTEIQCRVHDSRTGAGVVAGSVQQVPPAPQPVP"
     gene            2356..3435
                     /gene="asd"
                     /locus_tag="VF531_03215"
     CDS             2356..3435
                     /gene="asd"
                     /locus_tag="VF531_03215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7713007.1"
                     /translation="MIKVAIVGATGIVGQQAIVSLVNHPWFQIVKLAASERSAGKKYQ
                     DALKDANGASRWWCPEPMPEQLADLIVEDAGRLDPTSVDLIFSTVDASAAKELEPVYA
                     KTTPVISTASAFRYESDTPILVGGVNMEQAQMLAIQQEKRGWKGFVAPKPNCTTTGLV
                     VSLKPILDRFGVKQVIMTSLQAMSGAGRTPGLSAMDMTENVIPYIAGEEPKAESEPQK
                     ILGRFTGSTIEPAAFGVSATCTRVPVLDGHLVVAYVQTGKPATPEQIKEAMTGYGSEF
                     VELGLPSSAKHLIEVTDDPFRPQPRMDRERGGGMTVTVGRIRKDPLFENGIKYVCLAH
                     NTKLGAAKGAVQTAEYLAKYYLKWI"
     gene            complement(3554..4621)
                     /locus_tag="VF531_03220"
     CDS             complement(3554..4621)
                     /locus_tag="VF531_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7713008.1"
                     /translation="MNGEMLAEIKQQPEILKATMDKNRVILEEVRKELKPKIASGAID
                     NVMIVARGSSDNAAALGKYLIESTLGLPVNLAAPSVVNLYHAKLTLAKTLVIGVSQSG
                     STNEIVEFMSKVKADSGFTMSITNNPDSELAKLGLGANLFCFAGKEKVIAATKSFTST
                     VMLFYLISEALADETDGAKSSVLASIPDLIHETLAMAEPQIDDAVAWLGPSEECVILG
                     RGFSYPVALEAGLKLQECSYVRAKAYSGADFQHGPMAVIQERLHFIVLLAEGPCYQSM
                     IGLCRLILERGGRLLVFATQAEPELEAEGAKIIKLPACSEWVSPFVFTTAAQLLAYYV
                     AEGRGLNPDSPRWLNKVTATI"
     gene            complement(4635..>5217)
                     /locus_tag="VF531_03225"
     CDS             complement(4635..>5217)
                     /locus_tag="VF531_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7713009.1"
                     /translation="IGAGVIVNGSLYRGYHFSSGEIGYMVLERNFLGEDFDQHGFLES
                     RAAGPAFVRGLEKEWRFGSGPDLVGEVPEKKGSVTVEYVMAEAGRGDPAAVRVVEEIT
                     DYICMGVLNAVTVLDPEMIVLGGGVVRAGGLLVERVRGVIERTLPFAPEIRISALGDD
                     APLLGCVVAGLEELEPYLLIDSPEAARINVKLT"
BASE COUNT         1358 a         1411 c         1236 g         1212 t
ORIGIN      
        1 atatctacgc ccatatccag taaacgatcg atcaattttc gttgatcggg tgagggttga
       61 cgttgatact cggttccggt atgcatggcc atgatgattc catccgcccc gttacgcttg
      121 gcggctgcta tatcttcggc caccctcgaa aaatccagac ggttgactgc ccactcccat
      181 ccggacggag tagcaatgcc attagtgatc gtagtatacg agagaaatgc cagccgtatc
      241 cctttgactg ctacgatccg gtaacgagcc tgctttggtt ggcgttgccc gccgacatac
      301 ggcagtccga tctgatccag atacgccagg gtggactcga ggcccgaaat tccccggtcc
      361 aggcagtgat tgtgggcggt aaagaccaga tccaatccgg cccactttaa ggcatcggct
      421 atggctcccg gagcattgaa acgggggtag ccggtatatt tctcctccgg accggccagc
      481 ggagactcga gtacccccac ggccagatcg gaacggagca ggtcttgatt tattccggta
      541 aagatcggcc ggaaatcata tgcgccggtt gccgggtcaa aggccgaatc cacgattggg
      601 gtatgcataa ccacatcgcc cacggcggtc aaggttacaa cttgttcctc gaccgggacc
      661 atgccggttg aaacgggccg atccgcccca cggcaagtta cgccaaagca tagtaaaaag
      721 gtgattccca tcaacaaccg gaatcttttt aaattcaata tgctcccacc ctttttgcca
      781 tttccatttg ttttcaataa aaatatggca aatcaacgga tggaaactat ttctacggga
      841 tggtaatatt ctcctgccgc ggaactaaaa acagaatggt cgattgagat cataataaaa
      901 caaagcttta ttttcaataa tggttgatct acggaaacct ttttacatat gatagataaa
      961 atacttcaat ggctgcaatt aaagctattc ccattttcgt gtctttggtg tgtttcgttg
     1021 tgaataaaat aggcttacag atagaaactt cttaaccacg aaaatcacca aatacacgaa
     1081 atgtttcttc gggcgataaa aatttgcttc attccttcac agttatttgt aaaaagagat
     1141 aattataaat ggccgtattc gatcaaatat tttcgagaac atcgcgtaaa ataaaaccag
     1201 tcgccgtatg cacgagcgac tggttttgat atagaaagaa acggttcatc ctggaactta
     1261 cggtaccggc tgaggggcag gcggcacctg ttggacgctg ccggcaacca ctccggctcc
     1321 ggtccgggag tcgtggaccc ggcattgaat ttcggtaaaa cccggaagat ccgtaagata
     1381 accgtacatg acatagctga cacccaataa cttccccgct tccttggtga acttgacgga
     1441 acccggggta ctggtggcgc taagcatttt aacgatctgc tcggtccgga ggctgtccgc
     1501 cactacaaaa cggccggttt tgatcaattc gttaatcatt aattcctgag caacacgggc
     1561 tgtgatccca tccttggagt ttaggtattc aaacggcacc actgcaatgg agaatttacg
     1621 ggctttctcg acggcttttt gaccgaccgc caatggtaca gtggccacag tactttgacc
     1681 cctattgatt acttggccga tacagaggtt cgtgcggaca ctaatgatct taacatcagc
     1741 gacatgggtt tcaatcgtcc caatcctggc ccgggtcgta ggatcagtca ccggagcgcc
     1801 ctcagccacg atctcaaaga gtgttccttc cttgatatat ttcgtgcttc cggcattcag
     1861 gtagactgta tcaccttgaa ccgcagccac tctggcctcg gttactccaa actgccgggt
     1921 cacaagttga gccagattag cgatactggt tcccaaatcg ttttgagctc cataggcccc
     1981 gggagtaacg gcgcagatga tgatcaagag ccatatcccc aaaaccttcg gtacatacgc
     2041 gcgcagtaat cccttcacaa tcatcgtccc cccagaaaac agaaattcca gtccgagaca
     2101 atcatccttg atttgccaat attatcggcg gaaacggcgg ggaactttag gtttgaaagc
     2161 ttttcggccc ggtttaccgg atgaaataaa tccggaagat tcaggttatg tcaaggcgga
     2221 tgctttattt tatgcttccg aaagtgtata atttgaaagt acgtcatatg atgataaaaa
     2281 gctttgccta aacggcattc ttgtgataaa gtattaataa ttttaataca atgagtcaaa
     2341 attgggggta ttgaaatgat caaagttgca atcgtcggcg ccaccggaat cgtcgggcag
     2401 caagccatcg ttagcctggt caatcatccc tggttccaga ttgttaaatt ggcggcctcg
     2461 gaacgttcgg ccggtaaaaa gtaccaggac gcactgaagg atgcgaacgg cgcttcacgc
     2521 tggtggtgtc cggaaccaat gccggaacaa ttggccgatc tgatagtgga ggatgccggc
     2581 cgtctcgacc cgaccagcgt ggatctgatc ttttccactg tcgacgcctc agccgctaaa
     2641 gaattggagc ctgtctatgc caagaccacc ccggtaatca gcaccgcttc cgcattccgg
     2701 tatgaatcgg acacacccat cctggtcggc ggggtcaata tggaacaagc tcagatgctc
     2761 gccatccaac aagagaagcg cggctggaaa ggttttgtcg ctcccaagcc caattgtacc
     2821 accaccggtt tggtggtatc gctcaagccg atcctggacc ggttcggtgt gaaacaagtg
     2881 atcatgactt cactccaagc catgtcggga gcgggtcgga cccctggatt atcggcgatg
     2941 gacatgactg agaacgtcat tccctatatc gccggagagg aaccaaaagc cgagagcgaa
     3001 ccgcaaaaga ttttgggacg cttcaccggt tcaaccatcg agcccgcggc tttcggcgtc
     3061 tccgccacct gcacccgggt accggtactc gacggccatc tggtggtcgc ctatgtccag
     3121 accggaaaac cggctacacc ggagcaaatc aaagaggcca tgactggtta cggcagtgaa
     3181 tttgtggaac tgggtttgcc gagctccgca aaacacctga tcgaagtgac cgatgatccc
     3241 ttccggcccc agcctcgaat ggaccgggaa cggggcggcg ggatgacggt gacggtcgga
     3301 cggattcgga aagatccgct ttttgaaaat ggaatcaaat acgtctgtct ggcccacaat
     3361 accaaactgg gggctgccaa gggagccgtc cagaccgccg aataccttgc caaatattat
     3421 ttgaaatgga tttaaacgtc tgtacgagaa gaatctacga ctcttgcgga atatagaggt
     3481 ttgatcaaca aaaattggta aaaagggctg cgccgccatt ggcgcagccc tttttaatcg
     3541 atatcgtgca ggcttaaatt gttgccgtta ctttatttag ccaccgcgga ctgtcgggat
     3601 tgagcccccg cccctcggcc acataataag caagcagctg cgcggcagta gtgaacacaa
     3661 agggtgacac ccattcggaa catgccggca gtttgatgat tttagctccc tctgcctcaa
     3721 gttcgggttc ggcttgagtt gcaaagacca gcagacgccc gccgcgttcc aatatcaaac
     3781 ggcataaccc aatcatactc tgatagcacg gaccctctgc cagtaaaaca atgaaatgta
     3841 accgttcctg aatcacggcc atcggcccgt gttgaaaatc agcacccgag tatgctttgg
     3901 cccggacata ggaacattcc tgaagcttca aaccggcctc aagcgccacc ggataactga
     3961 agcccctacc cagaatgacg cattcctcgc ttggccccag ccaggcaacg gcatcatcaa
     4021 tctgtggttc agccatggcc aatgtctcat gaatcagatc gggaatcgaa gccaaaacgg
     4081 acgattttgc cccgtccgtt tcatccgcca aagcctctga aatcaggtaa aacagcataa
     4141 cagtactggt aaaagacttg gtagccgcaa tcaccttttc ctttcccgca aagcaaaaaa
     4201 gattcgcgcc taatcccagt ttagccagtt ccgaatcggg attgttggtg atactcatgg
     4261 taaaaccgga atccgccttt actttcgaca taaattcaac aatttcattg gtggatccgg
     4321 attgagatac accaatcact aaagttttcg ccaacgtaag cttggcatga tacaggttaa
     4381 ccaccgaggg cgcggctaag ttcacgggta aacccaaggt ggattcgatc agatacttgc
     4441 ccaacgcagc ggcattatcc gaactgcccc gggctacaat catcacatta tcaatggcgc
     4501 cagaagcaat cttcggcttg agttccttgc gaacttcctc caaaataacc cggttcttat
     4561 ccatcgtggc cttaagtatt tccggttgtt gcttaatctc tgccagcatt tccccgttca
     4621 tgaaaatatc ctccttatgt taacttcaca tttatgcgag cggcctctgg tgaatcaatc
     4681 aataaatatg gttctagctc ttcaaggccg gctacaacac atcctaatag cggagcatca
     4741 tcccccaatg cagagatacg aatctccggg gcaaacggca atgtccgttc gattacgccg
     4801 cgaacccgtt ccacgaggag tccgccggca cgaaccactc ccccaccaag cacgatcatc
     4861 tccggatcca ggacggtgac ggcatttaaa acacccatac agatatagtc ggtgatctcc
     4921 tctaccaccc ggacggccgc cggatcaccg cgtccggcct cagccatgac atattcgacg
     4981 gtaacagatc cctttttctc cgggacctcg ccgaccagat ccggacctga accgaaacgc
     5041 cactccttct caagcccccg aacgaaggcc ggtcccgcgg cccgggattc caaaaaacca
     5101 tgttgatcga aatcttctcc aagaaaatta cgctccagta ccatatagcc aatctcgccg
     5161 ctgctgaaat gataaccccg gtataacgaa ccattgacaa tgaccccggc gccgatt
//
LOCUS       DASVMB010000098         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_676058,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000098 DASVMB010000000
VERSION     DASVMB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676058"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..636
                     /locus_tag="VF531_03230"
     CDS             154..636
                     /locus_tag="VF531_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713010.1"
                     /translation="MVKDKVVISSIAGIIASLFKDLPNFILYKLGVVHYLYAHLAASA
                     HLLPDGVYFPLGWIIGFLGDTVTGGAIGVVTILFLDCFGTDYWWYKGLTIGLTIWLFG
                     LGVILNLGTVHLVPFDPVFRLTSIFDHIIFGEVTVYIIYRWQKGNERITERRIKNREG
                     "
     gene            715..1458
                     /gene="lgt"
                     /locus_tag="VF531_03235"
     CDS             715..1458
                     /gene="lgt"
                     /locus_tag="VF531_03235"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7713011.1"
                     /translation="MNSILLKLGPFQIHWYGVLIALAFLTGYWQTRRNAKNYGMDLDA
                     VDGLLLKLIIAIIVGARLAYVVSNYSYFMAYPSEIIRVDHGGLGSHGAIITAMVLGYF
                     WTKKAHVAYWSLGDAIAPAITVGHIFVRIGNFINGELYGPPTNLPWGVKFPTTPAPVH
                     PSQLYEVITSLIVLPFAIQWSKKPRYPGYAFLRVILVHSIIRFFMDFFRQSTSFSGRL
                     VLTQIIALIFTVVCIAYIWYLEAFKYPQH"
     gene            complement(1471..1938)
                     /gene="ribE"
                     /locus_tag="VF531_03240"
     CDS             complement(1471..1938)
                     /gene="ribE"
                     /locus_tag="VF531_03240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7713012.1"
                     /translation="MNIFEGKLVSKEIRVGIVAARFNEFITSKLLSGTLDGLKRHDVS
                     EDNIDVAWVPGAFEIPLIASKMAKSGKYDAVICLGAVIRGSTSHYDYVCNEVSKGIAQ
                     TALTSGVPVMFGVLTTENVEQAIERAGTKAGNKGFDCAESAIEMVNLIHTIEK"
     gene            complement(1953..>2868)
                     /gene="ribA"
                     /locus_tag="VF531_03245"
     CDS             complement(1953..>2868)
                     /gene="ribA"
                     /locus_tag="VF531_03245"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX7713013.1"
                     /translation="TTGISAEERGITARACVGENAKPEDFRRPGHMFPLMAKPNGVLE
                     RGGHTEATVDLLRLARLKECGLCCEVMSEDGRMMRMSELIQLVSKWGLTFITIKDLQD
                     YRKNHDKLVERVAVTKMPTRYGNFTAYGYVNKLNGEQHVALVKGEIGDGKNLLCRVHS
                     ECLTGDAFGSLRCDCGQQFASAMTQIEREGRGITLYMRQEGRGIGLINKFRAYELQDQ
                     GMDTLEANEALGFAGDLREYFIGAQILRDLGAKTLRLLTNNPDKVYQLADYGMEIIER
                     VPILMDATPYDLFYLKTKQVKMGHILNY"
BASE COUNT          664 a          732 c          660 g          812 t
ORIGIN      
        1 cagccgaaat atcggggaaa gacaaatcgt atatcaattt cctccattga tcatactacc
       61 tttagtcttt ttaagacgaa aactcctccg cacccgaatc gaccacaaaa attctacatg
      121 gacaagggtt ggggtttggg atgatctgcc aaattggtaa aggataaagt agtaatcagt
      181 tctatagcag ggataattgc gagtcttttt aaagatttac cgaattttat tctttacaaa
      241 cttggtgttg tccactacct ctacgctcat ctggcagctt ccgcccattt gcttcccgat
      301 ggagtatact ttccgctggg ttggatcatc ggtttcctgg gggacacagt caccggagga
      361 gcaataggcg tggtgaccat tttatttctt gactgtttcg gaaccgatta ttggtggtat
      421 aaaggtttaa ccataggttt gactatctgg ctgttcggct taggtgtgat ccttaattta
      481 ggtacggttc atctggttcc ttttgatccc gtctttcgcc ttacatctat attcgatcac
      541 atcatctttg gagaagttac tgtctatatc atttaccggt ggcaaaaagg aaacgagcga
      601 atcacagagc ggcgtataaa aaaccgggag ggctgaacat tagggtcttt tgtaattcac
      661 accaataaca aaattgactc caaagaaaaa gtgtgataat ttacaatata agctatgaac
      721 agtatcttat taaaacttgg accgtttcag atccattggt atggagtttt aatcgccctg
      781 gcgtttctga ccggctattg gcagacccga agaaatgcca aaaattacgg aatggatctg
      841 gacgcagttg acggcctgtt gcttaaactg atcatcgcca ttatcgtcgg ggcacgtttg
      901 gcctatgttg tttcaaatta ctcttatttt atggcttacc cttcggaaat catccgggtc
      961 gaccacgggg gcttggggtc ccacggggca attatcaccg cgatggttct gggttacttt
     1021 tggacgaaaa aggctcatgt cgcttattgg agtttgggtg atgccatagc gcccgcgata
     1081 acggtcgggc atatctttgt ccgcatcggc aattttatta acggtgaatt gtacggccca
     1141 ccgacaaact tgccttgggg cgtgaagttt cccacgactc ccgcgccggt tcatcccagt
     1201 caattgtatg aagtaataac ttccctaatc gtattgccct ttgccataca gtggagcaaa
     1261 aaaccgcgct atcccggcta tgccttttta agagtcattc tggtccactc gatcatccgt
     1321 ttcttcatgg atttcttccg gcaaagtacc tcttttagcg gccggctggt gttgacccag
     1381 atcattgcct taatctttac ggttgtatgt atcgcttaca tctggtattt ggaggctttt
     1441 aaataccctc aacattagtg cagagtatgt ttacttttct atcgtgtgga tcaggtttac
     1501 catttcaatt gcactctccg cgcagtcaaa acctttgttg cctgctttgg ttccggcgcg
     1561 ttcaatggcc tgctcaacgt tctccgtcgt gagcacgccg aacattaccg ggactccgct
     1621 ggtaagcgcc gtctgcgcta taccctttga cacttcattg caaacataat cataatgact
     1681 tgtgcttccc cggatcacag caccaagaca gatcaccgcg tcgtattttc cgcttttcgc
     1741 catcttcgac gcgatcagcg gaatttcaaa cgcaccggga acccacgcca cgtcgatgtt
     1801 gtcctccgat acatcgtgac gcttcagacc gtccagtgtg ccagacaaca gtttggaagt
     1861 aataaattcg ttgaagcggg cggctacaat gccgacccgg atttctttag ataccagttt
     1921 tccttcaaaa atattcattg tgaaagctcc tttcagtaat tcagaatatg tcccatctta
     1981 acctgtttcg ttttcagata aaacagatcg tagggggtgg cgtccatcag aatgggaacc
     2041 cgctctatga tctccatgcc gtaatcggca agttggtaca ctttatccgg attattggtc
     2101 agaaggcgca gcgtcttggc tcctaagtcc cggagaattt gcgcgccgat gaaatactcc
     2161 cgcagatcgc ccgcaaagcc aagcgcctcg tttgcttcca gcgtgtccat cccctgatcc
     2221 tgcaattcgt aagcccggaa cttattgatc agcccgatgc cgcgaccttc ctgccgcata
     2281 taaagcgtga tgccgcgacc ctcccgttca atctgcgtca ttgcggacgc gaactgctgt
     2341 ccgcagtcgc agcgaagaga gccgaaggcg tccccggtca ggcattcgga gtggacacgg
     2401 cacaacagat tttttccgtc tccgatttct cccttgacca gtgcgacgtg ctgttctccg
     2461 ttgagtttgt tgacatagcc atatgcggta aagttgccgt atcgggtagg catctttgta
     2521 acggcaacgc gctcgaccag tttatcatgg ttttttctgt aatcctgtaa atctttgatc
     2581 gtgataaagg tcagccccca tttactgaca agctgaatca gttcggacat ccgcatcatc
     2641 ctgccgtcct cactcatgac ttcacagcac aggccgcact ctttcagtct ggcaagccgt
     2701 agcagatcca cggtggcctc cgtatgcccg ccccgttcca gaacaccatt gggtttcgcc
     2761 atcaatggaa acatatgacc ggggcggcgg aaatcctccg gttttgcgtt ctcgccaaca
     2821 caggcacggg ccgtgatgcc tcgttcttcg gcggagatgc ccgttgtg
//
LOCUS       DASVMB010000099         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_379835,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000099 DASVMB010000000
VERSION     DASVMB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379835"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF531_03250"
     CDS             <1..228
                     /locus_tag="VF531_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX7713014.1"
                     /translation="VQVALTGRAEVHFLRTARAGEKLIAKAQVMEKDGHRFLIEVVVR
                     SKEDVVCRGEFVIHGMTLEMANYLKLLKDDE"
     gene            318..1277
                     /locus_tag="VF531_03255"
     CDS             318..1277
                     /locus_tag="VF531_03255"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7713015.1"
                     /translation="MSEIGIISIGAAVPQRILTNADLEKMVETTDEWIVTRTGIRERH
                     IMSPDETVAGLVTRAAKNACGRIGRNPQELDFVISSTLTPDRISPAQSFEVARGLEMG
                     HAFCFDMNAACSGFIYALAMAESLLKTRNISSGLVTAGEQLSTLTDYRDRSSCILFGD
                     AAVAALVTNDHPDHIIRYSELGSDPSLADEVVIGGVRDLMNDRRDDFYFRQNGKVVFK
                     FAVSKIKEICERVPRLAGLTIDDIEYIIPHQANMRIVEAASKEIASDKTQFLYNIAKF
                     GNTSSASIGLVLNDEWSRFQKGDHILLIGFGGGLSWGAALIEW"
     gene            1422..>2045
                     /locus_tag="VF531_03260"
     CDS             1422..>2045
                     /locus_tag="VF531_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7713016.1"
                     /translation="MSENTILITIIAIGLAILTSYHFHNRDFYRHLFEWEAAFDAGLD
                     FAHLTKSILKKVMEETSASAGIIYWFDEVQNEYKLKSLAGIPAEQINQVAGVLRQPGG
                     VLDRVRDGSGVYQIALRLVRNCPEAELAPSYRTLLVIPLIIEKRPLGSLFLFKTQGRF
                     TPRQQNLLTLFASRSAIRLENARLYRLAKETALENTKLYINLSKLYQQ"
BASE COUNT          501 a          460 c          535 g          549 t
ORIGIN      
        1 gttcaggtgg ctttaaccgg gagggctgag gtccattttc tccgtacggc cagggccggt
       61 gagaagctga tcgccaaggc ccaagtgatg gagaaagatg gtcaccgttt tctcatcgaa
      121 gtagtggttc gttccaagga ggatgttgtc tgtcggggag agttcgtcat tcacggtatg
      181 actctggaga tggccaacta ccttaaactg cttaaggatg atgagtaaga ttatccggtc
      241 attggaaggg ggcggaccgg ttatcctatg gatgaattcc ttggggccat tttatctatg
      301 ataatcagga ggaacatatg tcagaaatcg gaattatttc aatcggagcc gcagtaccgc
      361 aacgtatctt aaccaacgcc gatctggaaa agatggtgga gaccaccgat gagtggatcg
      421 ttacccggac gggaattcgg gagcggcata tcatgagtcc cgacgagact gtagcgggtt
      481 tggtcacccg ggccgccaaa aatgcctgcg gacggattgg gcggaatccg caggagcttg
      541 attttgtcat ttcctcaacc cttactccgg atcggattag tccggcccaa tcctttgaag
      601 ttgcccgggg attagagatg ggccacgcat tttgttttga catgaacgcc gcgtgctccg
      661 gctttattta tgccctggca atggcggaaa gtttgctaaa gacccggaat atcagttccg
      721 gcttggtaac tgcgggtgaa cagctctcca cactcactga ttacagggat cgttcctcct
      781 gtatactctt cggtgacgcg gcagttgccg cactcgtcac caatgatcac ccggaccata
      841 taatccgcta ttccgaattg ggatcagacc cttcgctggc tgatgaagtt gtgatcggtg
      901 gagtccggga tctgatgaac gatcgccgcg atgattttta ctttcgccaa aacggtaaag
      961 tggtttttaa atttgcggtc agcaagatta aggagatttg tgaacgcgtt ccgcgtctag
     1021 ccggcttgac aatcgatgat attgaatata tcataccgca ccaagccaac atgcgaatcg
     1081 tggaagcggc gtcgaaagag atcgcctccg acaagactca gtttctttat aatatcgcta
     1141 aattcggcaa tacctcatcg gcatccatcg gtttggtatt gaatgatgag tggtcccgtt
     1201 ttcaaaaagg cgaccatatc ttgttaatcg gatttggcgg cggactaagt tggggcgcgg
     1261 cactcatcga atggtaacca ttgaggttta ataagaattg tctttaaaaa gcggcttaaa
     1321 ccatattggt tttagggccg ctttttttat gcccggggga ttttcgactt aattaagtaa
     1381 ctgttcgtat tttgccgata atattggcaa ggagtgcttc aatgtcggaa aatacaattt
     1441 tgataacgat tattgcgata gggctggcga tattgaccag ttaccacttt cataaccgcg
     1501 acttttaccg gcaccttttt gaatgggaag ctgcttttga tgccggattg gactttgcgc
     1561 atttgacaaa gagtattcta aagaaggtaa tggaggaaac ttcagcttcg gccggtatta
     1621 tctattggtt tgacgaggtt caaaacgagt ataaattaaa atccctggct ggaatcccgg
     1681 cggaacagat taaccaggtt gccggggttt taagacagcc tggcggggtg cttgatcggg
     1741 tccgggatgg atccggtgtt tatcagatcg cgcttcgtct ggttcggaac tgtcctgaag
     1801 cggaacttgc cccaagctac cgtaccttac tcgtcatccc gctcattatc gagaaacggc
     1861 cgctgggttc attgtttctc ttcaagaccc aagggagatt caccccgagg caacagaatc
     1921 tattgacgct ctttgcatct cgctccgcta tccgcctgga aaatgccagg ctttatagat
     1981 tagccaagga gactgcgtta gaaaatacca agctctatat caacctttcc aaactctatc
     2041 aacag
//
LOCUS       DASVMB010000100         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_235282,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000100 DASVMB010000000
VERSION     DASVMB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235282"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..807
                     /locus_tag="VF531_03265"
     CDS             163..807
                     /locus_tag="VF531_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713017.1"
                     /translation="MEKIRLLIVDDHPLFRQGVRFFLESIPELDLAGELGNGSAALEF
                     LSRQSVDLVLMDLQMPGMDGVEVTRHIKEAWPDLKVLVLTSFGGWDKVYAALKSGAAG
                     YVLKDAQPEELLAAIKAVAAGGSYLGAQAATELLNRIGNETGTNSDDLPEPLTDRELE
                     VLKLLGQGMGNREIAAQLVVSEKTVKTHVANILQKLQVSSRTQAALYAIRNNLV"
     gene            825..1976
                     /locus_tag="VF531_03270"
     CDS             825..1976
                     /locus_tag="VF531_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7713018.1"
                     /translation="MNQRTGDVKNKLNNPPERDNIRKVLPLTTGFGGFFGLVVTFLIF
                     SMYGFPTSILPFIIGVPLFCACIAGLAGVIDIYINRFLTKIGIINSLKWQIIGIVFVI
                     LITMTLGLSIAAYFGFIKDQVPFVATTSIIGLIFGIIVGMIDHYLWRMRQKVLTLEIE
                     NKYLAELAEKDQQVQETTKNLIIAEERNRMARDLHDSISQGIHGIVYTIHSLRQELGN
                     GTQRTAEILDHLERTADATLSELKTMIMELKPSLMEEKGLTEAIRLNCDLLAERFGIK
                     MDLRLDSNTGLSPGQEIALYRIIQEALANIQKHAGAKQVQVSLESGEKQQVRLMIQDD
                     GRGFDMGQIHRGNGLNNMEARCLENHGTLKIITRPGAGTKIEARFPCCN"
     gene            2205..>2348
                     /locus_tag="VF531_03275"
     CDS             2205..>2348
                     /locus_tag="VF531_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7713019.1"
                     /translation="MEPFVSVENLVKRYANVTAVDGVSFTISRGEIFALLGPNGAGKT
                     TTLE"
BASE COUNT          656 a          485 c          605 g          602 t
ORIGIN      
        1 tgcagttgtt tatactcaat tgacggatgt ggaaacggaa atcaacggtt tacttaccta
       61 cgacagggag atcatcaaaa cggatatgga tttggtaaat caactcaatc ggagctgtac
      121 ttcaagttta aataacgata ctcaattaag caggtagaac ttatggaaaa gattcgcttg
      181 ttgatcgtag acgatcatcc gctctttcgg caaggggtcc gctttttttt ggaatccatc
      241 cctgaactgg atttagccgg ggagttggga aacggctctg ccgctttgga gtttctttcc
      301 cggcaaagcg ttgatctggt tttaatggat ctgcagatgc cggggatgga cggggtggag
      361 gttacccgtc atatcaaaga agcgtggccg gatctcaaag tcctcgttct gaccagtttc
      421 ggcggctggg ataaggtcta tgcagccctc aaatccggcg cagcgggata cgttttgaaa
      481 gacgcccaac cggaggagtt gttggcagct attaaagcgg tggctgccgg aggcagctac
      541 ctgggggctc aggcggcaac ggagttatta aaccggatcg ggaacgagac cgggacaaac
      601 agcgacgatc ttcccgaacc cttaacggac cgtgaattag aggttctaaa gttactcggc
      661 cagggtatgg gcaaccggga gatcgccgca caacttgtgg tcagtgaaaa aacggtaaaa
      721 actcatgtgg ccaatatttt gcaaaagtta caggtgtcta gtcgcactca ggcggcattg
      781 tatgccatcc ggaacaattt agtctagcga tgggggaaag caatttgaat caacgaacgg
      841 gcgatgttaa aaacaaactc aataatccgc cggaacggga caacatccgc aaagtcctgc
      901 ctttaaccac cggatttggc ggctttttcg gattggtggt gacgttcttg attttttcga
      961 tgtatggttt tccaacatca attctgccgt tcattatcgg tgttccgctt ttttgtgcat
     1021 gcatagctgg tttggccggt gtgatcgata tctatatcaa ccgttttctg acaaaaatcg
     1081 ggattatcaa ttcgttaaaa tggcaaatca tcgggattgt ctttgtaata ttgattacaa
     1141 tgactttagg tttatccatt gccgcatact ttgggttcat caaagatcaa gttcctttcg
     1201 ttgcgacaac gtcgattatc ggattaatat ttggaattat tgttggaatg attgatcatt
     1261 atttgtggag aatgcgccaa aaagtattga ctttagaaat tgagaataag tatctggccg
     1321 aattggccga aaaagaccaa caggtccagg agaccaccaa aaacctgatt attgccgagg
     1381 aacgcaatcg catggcccgg gatttgcatg attcaatctc ccagggaatt catggcattg
     1441 tctacaccat tcattcactc cggcaagagc ttggaaatgg gactcaaagg acggccgaga
     1501 ttttggatca tctggaaaga accgctgatg ccactctgag tgaattaaaa acgatgatta
     1561 tggagttaaa accttcctta atggaggaaa aaggtttgac ggaagcgatc cggttgaact
     1621 gcgatctctt ggcggagcgg ttcggaatca aaatggatct acggcttgac agcaatactg
     1681 gactctcacc gggacaggaa atcgctctat accgaattat ccaggaggcc ttggcaaata
     1741 tccaaaaaca cgccggagcg aaacaggtac aagtatcact tgagagcggg gagaagcaac
     1801 aggtaaggct gatgatccaa gatgatggca gaggttttga tatggggcag attcaccgcg
     1861 ggaatggact caataacatg gaagcccgtt gtctggagaa ccacggaacg ctaaaaataa
     1921 taacgcgacc gggtgccggg acgaagattg aagcgagatt tccctgctgc aactgatttg
     1981 tcatacttat gccaatgatt gcgtaataaa gactgatcca tgagatcagt ctttttattt
     2041 accccaaaat acatctttcg gctgaatgaa atggcactga tctctgataa agtatgttta
     2101 aagtcttaag ggtgataggg aataggcaac agggtagtaa cagtttccct gttgcctatt
     2161 gcctgacccc tattgcctta taaacaaaag ttaggagcaa aagcatggaa ccgttcgtat
     2221 ctgtggaaaa cctggtaaaa cgctacgcta atgtcacggc ggtcgatggt gtcagtttca
     2281 ccatctctcg cggcgagatt tttgcattac tggggccgaa tggcgccgga aaaacgacga
     2341 ccctggag
//
LOCUS       DASVMB010000101         9109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_676312,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000101 DASVMB010000000
VERSION     DASVMB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676312"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VF531_03280"
     CDS             complement(<1..501)
                     /locus_tag="VF531_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713020.1"
                     /translation="MKKMKSSLWIAICLSGALLISSNLAFAEDVTPSEATPSEDSLFS
                     GDDTVVSSDQVVNDTTGDELQQKHLGFSGEINAATAYTNYSSDYDWTGKGGPETDQLS
                     NQIAADFFLDIRLQKGIKGFLSFEADYYPAISPQELGLSGTDPQSITNLAIKEFFVDS
                     NWNNKVY"
     gene            complement(514..1287)
                     /locus_tag="VF531_03285"
     CDS             complement(514..1287)
                     /locus_tag="VF531_03285"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7713021.1"
                     /translation="MNKKVLISFVLLLAMVLPNTVFAAPKIDDILGTIDRLSELKSDG
                     TAKVEITQQKAGEGTKLYQSIYYRRDKDDSFLIIMTAPEVEKGNGYLKQGDNFWMYRR
                     NTRTFQHINRDESIAGTDSKGQDFESKKLTEMYKAATDASGKEKIAETKLGEIPVYQF
                     EVTAKIEDVSYPKQVYWVRQDNFLPLKIQSYSLSGTLMQTAYFMKYTQIEGKYMWIQG
                     MFIDEFEKGNKTLVNIKSISLQKIDDSVFTKAYLENLSK"
     gene            complement(1310..3043)
                     /locus_tag="VF531_03290"
     CDS             complement(1310..3043)
                     /locus_tag="VF531_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7713022.1"
                     /translation="MGLIYRLSLRNLLRQKKRNLLLGIGIAFGMMILVVANSFSHGMV
                     DVLIKDIVSNAFGHLVVQASQGNSSYTMIRDKERIMQVVKANIKSEDLVGVGENLGMF
                     CQAVGNGASDNIMVVWVSVQSAKERKEFFNDFFTLKQGNFDEYFSKNIEYPVIISEQT
                     AKSLNVKVHDAIKIRLPMVTGQLQTAQLVVVAIANANNSFMNVVAFMDGDRVKSLLGY
                     KPWESASLHITLKDPARTAKKYADILHEKLQPGLLSISGNVNHQACPLFAFKNDEASK
                     SVLRRNIRIVSGDADLAFSKDGVLIPLGLARKLNLRPGDSFSYEYPTKYRGLYQENLK
                     VSAIYDSSTKLGSDLILVNGERIYDTYDKYLPQTAAVDPVSPKDPLHAVLATEWKLLP
                     RCKDSTELQKLSKEERRIKTDQSKFSVVTMYEGASDILNLEGVLNLITVIAVMVLFFI
                     ILIGVINTLRMTVRERTREIGTIRAIGMQKNDVRNLFIMETLQLTAISCVAGFVLGLG
                     VMWILGSITFNTDSALSMILKNKHLFFKLDPVGLVTNFLLIMVIAGVTAYFPARRAAK
                     LSAMEALRHYE"
     gene            complement(3062..4585)
                     /locus_tag="VF531_03295"
     CDS             complement(3062..4585)
                     /locus_tag="VF531_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7713023.1"
                     /translation="MGLAFKLAWRNIWRHRGKSLIIGIILFCGALLMTVGNGMISGME
                     KGLSENIVNLFTGDVVVISDEQEQNDVLFQMMGKPLKVIKDYPAAQKVLAADQNIQQF
                     LPATAGMVMVLNTGSEMGNIMLLGVDIEKYRQMFPGSIAVTEGRMFKPGERGVLVSEE
                     ARKQAFDYMDFWVLPEGETFDSKKLPADAQTNIAGLDLRGDLVFMGASTSNSAMDIRV
                     PVTGIIRYKSLNKIWGMYCIVDIESFREAHNYVTGADRQAVIPKEEKALLDSDNLDQF
                     FSTGDMVDTSSVTSQAISIQDIKTQTKKTEPTTNIDNGSYNLAFIKLKPGVSQQAAIR
                     ELNNTFKENHLKLRAISWKESVGTIGSMAVLVKTALNVFIMFIFFVAIIVIMNTLSMA
                     ALERVSEIGMMRAIGARKGFLRSMFINETGILSFFFGGLGIIAGIIIIYLVQAAHITT
                     TNEILQLVYGGDQLRPIFTSGDLLLGIVELCIVTFLSVIYPLRVVGKIVPLDAIARE"
     gene            complement(4591..5271)
                     /locus_tag="VF531_03300"
     CDS             complement(4591..5271)
                     /locus_tag="VF531_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713024.1"
                     /translation="MNLIEVKAVQKNYPLGKTTVHALRGVNLTVAEGAFLSIIGPSGS
                     GKTTLLNIIGCIDTPSSGSVKVHDEEITQLSDKKITDLRLNKIGFIFQTFNLIPVLNV
                     RENVEFPLLLMKKHSKEEISSRVEKLIREVGLFDYIEHKPAELSGGQRQRVAIARALV
                     TQPDIVLADEPTANLDSATSQSILELMKELNEKEKTTFIFSTHDPDVLKYAKSIVKIK
                     DGLIVEQG"
     gene            complement(5878..6717)
                     /locus_tag="VF531_03305"
     CDS             complement(5878..6717)
                     /locus_tag="VF531_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7713025.1"
                     /translation="MIRVVTDTTSSLTLEQYRRYGITAVPLYVRRDEVIKKELYELSY
                     EEFYREQRAGVKFFTSQPDPQSFLEVFRQIIEAGDEVVCVTISSKISGTINAANLAKE
                     LLASDGITIFDSQESGINQAAMAIRAAEMAGAGESRATITAALEAMRARKRVFFVVET
                     LRYLYEGGRLNGIQALIGSIIQLKPIVWFDNAGVMTSLEKVRTLKAAKARSLELIREQ
                     AKLGVEQVGLHYGDNLEEAKEFAGEFEKIVGMPVPLVQVSPVIAVHTGPDLLGPCIIT
                     KKV"
     gene            complement(6861..>9109)
                     /gene="pulA"
                     /locus_tag="VF531_03310"
     CDS             complement(6861..>9109)
                     /gene="pulA"
                     /locus_tag="VF531_03310"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEX7713026.1"
                     /translation="TLNALSNRIEKAVITGPDEIEVSARAPLAKTLHVLDQGQEVKVI
                     AVATDKAPVYRIKTAANLDLTKNYTVTDGKLTTTTSVAPELLDRLYAFDGWLGNQYSP
                     SATTFRLWAPLALDVKLLVYQKGEDVKPLARFEMKQGEKGVWSVKASGDLAGKFYQYV
                     VNNGGQYKMVLDPYARSMAAFNSDGLDKVGKAAIIDLSKTTPQGWAGDRYVTLKAQED
                     AIIYETSVRDFTIAANSGVSPELRGTYRGFIAKIPYLKELGITHVQLLPVQNWYYGNE
                     SNRQFEAEAGPPANYNWGYDPHNYNTPEGWYSTNPQDPYARVRELKELIQALHSQGIG
                     VVLDVVYNHTANTNILEDIVPNYYYRRDARGSFTSGSGCGNDTASERAMWRKFMIESC
                     TYWVKEYHVDGFRFDLAGLHDDETMKQVAAACRAINPSIELHCEGWDLGTLPYAIRYT
                     KGGGPVDNFKRSCLTMDHAIGMFSDGIRDGILQTSYTDLRGGSFIQRQQNDEAKIRAG
                     VIGSIVHYQTSLPINTDPYHRFCDDPEESVNYTNCHDGLTLWDKTNATIPKASLDEKI
                     RTNQLAIAIIMTSQGKAFFQSGEEMLRTKPDPGNPNGLGVSSNSHDQGDSVNQIDWSR
                     AEAYPEVVSYYKGLIAMRKAHKAFRMETEEEIERSLTFIDEQIDFLLALKLIDPSGAD
                     PWKEIVVIYNANRTPQTLKIPGVDSSWRVAVDGKQATSGELTRTEAEIGVGSVTVPPV
                     AAVVLYKM"
BASE COUNT         2214 a         2368 c         2153 g         2374 t
ORIGIN      
        1 atagaccttg ttgttccagt tggaatcgac aaaaaattcc ttgatggcga ggttggtgat
       61 actttgagga tcagtaccgg aaaggccaag ctcttgagga ctaatagccg gataataatc
      121 ggcctcaaag cttaaaaagc ccttaatgcc cttttgcaac cggatatcca aaaagaaatc
      181 cgccgcaatt tgattggaga gttgatccgt ttccggaccg cctttcccgg tccagtcgta
      241 gtcggaagaa taattggtat acgccgtggc ggcgttgatc tctcccgaga acccgagatg
      301 tttctgctgc aattcatcac cggtggtgtc attgacgact tgatccgaac tgacgaccgt
      361 gtcgtcgccg gagaataagg aatcttcgct gggggtggct tcacttgggg tgacatcctc
      421 ggcgaaagcc aaattggaag atattaaaag cgcgccgctc aaacagatcg cgatccacag
      481 ggaacttttc atttttttca tgaacgtcca cctctatttg ctcagatttt ccagataggc
      541 cttggtgaat acggagtcgt caatcttctg gagtgaaatg ctcttaatat tgaccagggt
      601 cttgttgccc ttctcaaatt catcgatgaa catcccttgg atccacatgt acttcccctc
      661 gatttgcgta tacttcatga agtaagcggt ctgcatcaaa gtcccgctca gggagtagct
      721 ttggattttt aggggtaaga agttatcctg gcgtacccaa tacacttgct tggggtagga
      781 gacatcctcg atcttggcgg taacctcgaa ctggtagacc gggatctccc ctaatttggt
      841 ctcggctatc ttttccttgc cggaggcatc cgtcgccgct ttatacatct cggtcaactt
      901 tttgctctca aaatcctgcc ccttggaatc ggtgccggcg atgctttcat cgcggttgat
      961 atgctgaaag gtgcgggtat tgcggcgata catccagaag ttatcgcctt gcttcaagta
     1021 gccgttgcct ttttcgactt caggggcggt catgatgatt aaaaaggagt cgtccttatc
     1081 gcggcggtaa taaatggact gataaagctt cgtgccctcg ccggctttct gttgcgtgat
     1141 ctcgaccttg gctgtgccgt cgctcttcaa ttcgctgagc cgatcgatgg tacccaggat
     1201 atcatcgatt ttgggtgcgg cgaagactgt attgggtaaa accatcgcca gtaataaaac
     1261 gaaactgatc agtactttct tattcaaatg aaataccccc tcaagatttt tactcataat
     1321 gacgcaacgc ttccatcgcc gatagcttgg cggcgcgccg ggccgggaaa tacgcggtaa
     1381 ctccggcgat aaccatgatc aacaagaagt tggtgacgag tcccaccggg tccagtttga
     1441 agaagagatg tttgttcttc aggatcatgc taagtgcgct atcggtattg aaggtgatcg
     1501 aacctaaaat ccacatcact ccgagtccca ggacaaaacc agccacacag gatatggctg
     1561 tcaactgaag cgtttccatg ataaacaaat tacggacatc atttttctgc atcccgatag
     1621 cccggatggt cccgatttca cgggtccgtt cccggacggt catgcggagc gtattgatga
     1681 cgccgatgag aataatgaag aacaacacca tcacggcaat gactgtgatc aggttgagga
     1741 caccctctag atttaagata tcgctggccc cttcatacat cgtgaccaca ctgaatttgg
     1801 actggtccgt cttgatccgc cgttcctctt tggaaagctt ctgcagctca gtgctgtctt
     1861 tgcagcgggg cagcaacttc cattcagttg ccagaacggc gtggagcgga tctttggggc
     1921 ttaccggatc gaccgccgca gtttgcggca agtacttgtc ataagtatca taaatccgtt
     1981 caccattcac caagatcaga tcggaaccca acttggtaga ggaatcatat atggcgctga
     2041 ccttgagatt ctcctgataa agaccccggt atttggtagg atattcatag gaaaatgagt
     2101 cacccggccg caaattcagt ttacgggcaa gcccaagcgg aatcagaacc ccgtctttgg
     2161 agaaagccag gtcggcatcg ccgctgacaa tacggatatt ccggcgtaat acgctcttag
     2221 aggcctcatc atttttgaag gcaaacagcg gacatgcctg atgattcacg tttccggaaa
     2281 tcgagagcag ccccggctgt aatttttcat gcaggatatc ggcatatttt tttgccgtcc
     2341 gcgccggatc ttttagggtg atatgcagac ttgcggattc ccagggctta taacccaaca
     2401 gcgacttgac ccggtcccca tccataaatg ccaccacatt cataaaagaa ttgttggcat
     2461 tggcgatggc caccacgacc agctgggcgg tttgaagttg tccggttacc atcggcaacc
     2521 ggatcttgat tgcatcatga actttgacat tcagcgattt ggctgtttgt tcggagatga
     2581 tcaccggata ctcaatattc ttactaaaat actcatcgaa atttccttgt ttcaaggtga
     2641 aaaaatcatt gaaaaattct tttctttcct tggcgctttg gacggaaacc cagactacca
     2701 tgatattgtc ggatgcgccg ttacctaccg cctggcagaa catgcccaaa ttttcaccga
     2761 cgccgaccag atcctcagat ttaatgttgg ccttaaccac ttgcatgatc cgctctttat
     2821 cccggatcat tgtataactg ctgttacctt ggctcgcttg gaccaccaag tgcccgaaag
     2881 cgttagacac gatatccttg attaagacgt cgaccatccc gtgggaaaac gagttggcga
     2941 cgaccagaat catcatgccg aaggcgatcc cgattcccag gagcaggttt cgtttctttt
     3001 ggcggagcaa attccgcaag cttaaccgat agatgagccc catggttata cccccaaaat
     3061 attattcgcg cgcaatagcg tccagcggaa cgattttacc gacaacccgc aagggataga
     3121 ttaccgatag gaaggttacg atgcataact ccacgattcc cagcagcaga tctccggaag
     3181 tgaaaatggg gcgcagttga tccccgccgt acaccaattg gagaatctcg ttagttgtcg
     3241 taatatgggc cgcttgcacc agataaataa tgatgattcc ggcgatgatg cccagtcccc
     3301 cgaagaagaa ggagaggatc ccggtttcat taatgaacat gctgcgtaaa aatcccttac
     3361 gggcgccgat ggcacgcatc attccgatct cgctgacccg ctccaaagcc gccatgctta
     3421 aggtgttcat gatgacgatg atcgccacaa agaagataaa catgatgaag acgtttaaag
     3481 cagtcttaac caatactgcc atactgccga tagtccccac cgattccttc cagctaatgg
     3541 cccgcagttt taggtggttt tccttaaagg tattgtttaa ctccctgatg gccgcctgct
     3601 gcgatacacc cggtttgagt ttaataaagg ccagattata ggatccgttg tcgatatttg
     3661 tcgtcggttc cgtcttcttc gtctgggttt taatgtcttg gatagagata gcctgactgg
     3721 tcactgacga tgtatccacc atatcccctg tagagaagaa ctggtccaga ttgtcgctat
     3781 ccaataatgc cttttcctct ttgggaatta cagcctggcg atcggcgccg gtgacgtaat
     3841 tatgcgcttc ccggaacgac tcgatatcga cgatgcaata catgccccag atcttgttca
     3901 atgatttata gcggataatc ccggttaccg gaacccggat atccattgcc gaattggagg
     3961 tactggcccc cataaaaacg agatcaccgc gcagatcgag acccgcgatg ttggtctgcg
     4021 catcagcagg caatttcttg gaatcgaagg tctccccttc gggaagtacc cagaagtcca
     4081 tatagtcaaa ggcttgttta cgagcctctt cggacaccaa taccccccgt tccccgggct
     4141 tgaacatccg gccttcggtg acggcgatac tgccgggaaa catttggcgg tacttttcaa
     4201 tatccacccc caacagcatg atattgccca tttccgaacc ggtgtttaaa accatgacca
     4261 tgcccgcagt ggcaggaaga aattgctgaa tgttttgatc tgctgccagt actttttggg
     4321 cagccggata atccttaatg acctttaaag gtttacccat catctgaaat aagacgtcgt
     4381 tctgttcctg ttcatccgag atgaccacga catccccggt gaacagattg acaatattct
     4441 ccgacaaccc tttttccatg ccggagatca tgccgttgcc gacggtcatc agcaaggcgc
     4501 cgcagaacaa aataatcccg ataatcagac ttttaccccg gtgtcgccat atattacgcc
     4561 aggccagttt gaaggctaat cccatgaagc tcacccctgt tcgacgatta aaccgtcttt
     4621 gattttgacg atgcttttcg catacttcag tacatccgga tcatgggtcg aaaagatgaa
     4681 ggtggtcttc tccttttcat ttaattcctt catgagttcc aggatcgact ggctggttgc
     4741 cgaatccagg ttggcagtgg gttcatcggc cagtacaatg tcgggttggg tcaccagagc
     4801 cctggcgata gccacccgct gacgctggcc gccggagagc tccgccggtt tatgctcgat
     4861 gtaatcaaag agtccgacct cgcggatgag tttttcaacc cgggaggaga tctcctcctt
     4921 ggaatgcttt ttcatcaaaa gcagtggaaa ttcgacgttt tcacgcacat tcaacactgg
     4981 aatcagattg aacgtttgaa agataaaccc gattttattc aggcgcagat cggtgatctt
     5041 cttatcactc aactgcgtga tctcttcatc gtggacctta acgcttcccg aggaaggggt
     5101 gtcgatgcaa ccgatgatat tcagcagtgt agttttgccg ctacccgacg gcccgataat
     5161 ggataaaaac gctccctcag ccacggtcag attgaccccc cgtaaagcat gaaccgtggt
     5221 ttttcccagc ggataatttt tttggaccgc cttgacttca atcaaattca tggtggttgc
     5281 ccctttcaga ttttttggcg atatgatatt cggtgatgat attactgcga tttacctata
     5341 ctccatcata ggatggatat acaaattata ccaagtaaat gttaaaatca actaaagatg
     5401 atcattacaa atgcaaaaca aataatagtc aattccatga caatagtcat caaactgcaa
     5461 aaaatattta atattttaag tgactttaaa taaaaatcga aaaattctcg taagaatcat
     5521 cattatgaca cctcccgcct tcaaaggcaa acccttcata tgatgtcgaa ccggttatag
     5581 attggtcgac ttccttttct ctatctactt acggataaaa acggcaaatg tctgaaagat
     5641 ttttaaaaaa taatcatccc gactgacatt atccaccggc attccatttc ttgctgtaac
     5701 aaccccaatc ctttggtgat ctattcccta ggagcaataa acatatcacc tatgacgcta
     5761 cggcccatta ggtatggaat tgcaataata aagcccgccg tagcgagctt tgggttttta
     5821 tattcggttt atcagactac tgccttttga agtgtttcaa acttcacact tcactcttca
     5881 aactttcttt gtaataatgc atgggccaag cagatccggt cccgtatgaa cggcaattac
     5941 cggcgaaact tgtaccagcg gtaccggcat tccgacaatc ttttcaaact ccccggcgaa
     6001 ctccttcgct tcctccaagt tgtcaccgta atgcagtccc acctgctcca ctccgagttt
     6061 ggcttgttcc cgaatgagct ccagcgaacg ggctttggcc gcctttagag ttcgaacctt
     6121 ctccaaggag gtcattaccc ctgcgttgtc aaaccacaca atcggtttca gttgaatgat
     6181 cgaaccgatc aaggcctgaa tcccgttgag ccgtccgccc tcgtagagat atcgcaatgt
     6241 ctcgaccaca aagaagactc ttttgcgggc acgcatagct tccagcgcgg ctgtgatcgt
     6301 tgcccggctt tcgccggcgc cggccatttc cgcagcccta atggccatgg ctgcctggtt
     6361 gataccggac tcctgcgaat cgaagattgt aatcccgtcg cttgccaaaa gttctttggc
     6421 caaattggcg gcgttgatcg tccccgatat cttgcttgag atagtcacgc agaccacctc
     6481 atcaccggcc tcaatgatct ggcgaaagac ttccaggaac gattgggggt caggttgaga
     6541 cgtgaaaaat ttgacccccg cccgttgctc cctgtaaaac tcctcatatg aaagttcata
     6601 gagttctttc ttaatcactt catcccggcg tacatagagc ggtacagcgg ttatcccgta
     6661 gcgccggtac tgttccaagg tgagagatga agtggtatcc gtaacgactc tgatcatccg
     6721 atctcctcca gtttcaaaat ccacagaaaa actccctata agtaaaactc ctgaaggaga
     6781 tgaattcttt cacttttggc atttttttat gacagcctta ggaccaaatt tatatctcat
     6841 ttcaaacttt gcacttcaat tcacatttta taaaggacca ctgccgccac aggcggtacg
     6901 gtaacggaac ccactccgat ctccgcttcg gtccgggtta gttcaccgct cgtggcctgt
     6961 ttgccgtcga ctgccacccg ccagctggag tcgactcccg gaatcttcaa ggtttgcggg
     7021 gttcggttgg cgttgtatat caccacgatc tccttccacg gatcggcgcc gctcggatcg
     7081 atcagtttca aggcgagcag gaagtctatc tgctcgtcga taaaggtcaa gctccgttcg
     7141 atctcttcct cggtctccat ccggaacgcc ttatgcgctt tacgcattgc gattaagcct
     7201 ttataatacg aaaccacctc tggatatgct tcggcgcgcg accaatcgat ctgattgacg
     7261 ctatcccctt gatcgtggga gttggaggaa actcccaaac cattgggatt accagggtcg
     7321 ggtttcgtac gcagcatctc ctcgccggat tggaagaagg ccttcccttg cgaggtcatg
     7381 atgatcgcaa tggccagctg attggtacga atcttctcat ctaggcttgc cttggggatt
     7441 gtcgcgttgg ttttatccca gagggtcaag ccgtcatggc agttggtata gttgaccgat
     7501 tcctccggat cgtcgcaaaa acgatggtag ggatcggtat tgatcggcag actggtttga
     7561 taatgaacaa tgctgccgat gacaccggcg cggatctttg cctcatcgtt ctgctgccgt
     7621 tggatgaagg agccgccgcg caagtcggta taggaggttt gcaggatccc gtcccgtatc
     7681 ccgtcgctga acatgccgat ggcatgatcc atcgtcaggc agctgcgttt gaagttatcc
     7741 acgggaccac cgcccttggt ataacggatc gcatacggca gagtaccaag gtcccatccc
     7801 tcgcaatgca attcgatgga ggggttgatc gcccggcaag cagcggctac ttgtttcatc
     7861 gtctcatcat catgcaaacc tgccagatca aagcggaacc cgtccacatg gtattccttg
     7921 acccaatagg tgcaggactc gatcatgaac ttccgccaca tggcccgttc cgaggcggtg
     7981 tcgttgccgc aacccgaacc ggaagtgaag gaaccccggg cgtcacggcg ataataatag
     8041 ttgggtacga tatcctccaa aatattggta ttggcggtat gattatagac aacatccaac
     8101 actaccccga tgccttggct gtgaagagcc tgaatcagtt ccttcagctc acgaacccgg
     8161 gcgtacggat cctgcggatt ggtcgaatac cacccttcgg gtgtattgta gttatgagga
     8221 tcgtaacccc agttataatt ggcaggcggc ccggcctcgg cctcgaattg tcggttggac
     8281 tcatttccat aataccagtt ttggaccggc agtagttgca catgggtgat acccagttct
     8341 tttaggtaag gaatcttggc gataaacccc cggtaggttc cgcgcaattc ggggctgacc
     8401 ccggaatttg cggcgatagt aaaatcgcga accgaagtct cgtagataat cgcgtcttcc
     8461 tgcgccttta gagtaacata gcgatcgccg gcccaacctt gaggagttgt tttagagagg
     8521 tcgatgatgg cagccttgcc gactttatca agaccatccg agttaaaagc ggccatcgac
     8581 cgggcgtacg gatccagcac cattttgtat tggccgccat tgttcacaac atattgataa
     8641 aacttaccgg ccaggtcgcc cgaagccttg acggaccaaa cccccttctc cccctgcttc
     8701 atctcgaaac gcgccaaggg tttcacatct tcaccctttt ggtaaacgag caacttgacg
     8761 tccagcgcca aaggagccca tagccggaag gtggtggcgg agggtgagta ctggttaccc
     8821 agccagccgt cgaaggcata gagccggtcg aggagttccg gcgcgacact ggtggtggtg
     8881 gtcagtttac cgtcggtcac ggtatagttt ttcgtcagat caagatttgc tgccgtcttg
     8941 atccggtaga ccggagcttt gtcggtagcg acggcaatca cttttacctc ttgcccctgg
     9001 tccaaaacat gcaatgtttt agcaagcggt gcccgggcgc tgacttcaat ctcgtccgga
     9061 ccggtgatca cagccttttc gattcgattg gataatgcat tcaatgtct
//
LOCUS       DASVMB010000102         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_380146,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000102 DASVMB010000000
VERSION     DASVMB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380146"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VF531_03315"
     CDS             <1..831
                     /locus_tag="VF531_03315"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7713027.1"
                     /translation="ILYFSGTGNTKYISEQIGEAFQRLNYHVDLISVEDGEALLKLNI
                     EEKLIGIGFPCYALSYPNIINQAIRKLPSSIKAVPAFIFATKGWSTGNSLKELARFIH
                     SKNLYPIQTASFVCPNNGWITLFSREFVLCRNMGYPKTLSTEIDGFAVNIINSMKRFE
                     REPFSIGTYGNPLVTPVTRLLKVMEGRVLRNYRVERERCISCGLCIKNCPDRNIRFDD
                     DRRVIFQNPDHCTGCLRCINDCPKDAIRLGGLITGKGWYTKELQAEYIKKATENRGIG
                     "
     gene            1059..1877
                     /locus_tag="VF531_03320"
     CDS             1059..1877
                     /locus_tag="VF531_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7713028.1"
                     /translation="MDQLLFGISGLPVGDQSPKRNYRSGIAYLKTAGLDAMELPFVRN
                     VNVTDKNKDEILQEKLDKDFFLSAHGSYFINLNADETEKQQQSMERIIKGAKALKSVG
                     GNSLVFHPGFYLNDSPEVTYATIKENLMKLPDLGVAYRLETTGKPTQFGSLDELIGLC
                     NEVPTCRLCVDFAHIHARGGGSLKTYDDYTAILAKIRERLGEAALFDMHIHLSGINYG
                     AKGEKNHLSFAESDFNYTDCLKALKDYKVKGCVICESPILEKDAALLKETYNRL"
     gene            complement(1998..>2444)
                     /locus_tag="VF531_03325"
     CDS             complement(1998..>2444)
                     /locus_tag="VF531_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7713029.1"
                     /translation="DGSGYPRGLKGEEIHLFGRIIAVADVYDACISPRKYRPRMTPYE
                     ALKNLLGESRLFDIRVLKSLVTSMAIYPIGSLVRLNTGEVAKVIGINHGYPFRPEIRI
                     LLDRTQQKLEQPIRLNLADEEYTQTYIEETLEHDESEKMYQLLEAV"
BASE COUNT          638 a          599 c          600 g          607 t
ORIGIN      
        1 atcctgtact tttccggaac cggcaatacc aagtacatct cggagcagat cggcgaggcc
       61 tttcaaaggc tgaattatca tgtggatttg atctccgtcg aggatgggga ggcactgttg
      121 aagctgaata tcgaggaaaa actcatcgga atcggcttcc cttgctacgc cttatcatac
      181 cccaatatca tcaaccaagc gattcggaag ctcccatcct ccatcaaagc ggttcccgcc
      241 tttatctttg ccaccaaagg ctggtccacg gggaacagcc taaaggaact ggcccgattc
      301 atccacagca aaaaccttta ccccattcag actgctagtt ttgtctgccc caacaacggg
      361 tggatcaccc tgttttcccg ggagtttgtt ttatgtcgaa atatgggata tcccaaaaca
      421 ctctctaccg aaattgacgg ttttgcggta aacatcatca actccatgaa gcgatttgag
      481 cgagagccgt tttccattgg aacttacggg aaccccttgg ttaccccggt cacccggttg
      541 ctgaaagtga tggaaggccg ggttctacga aattaccggg tagaacggga gcgctgtata
      601 agttgcggat tatgcatcaa gaactgtccc gatcgtaata tccggtttga tgatgatcgg
      661 agagtgattt ttcaaaaccc cgaccattgt acgggctgcc tacggtgtat caacgactgt
      721 cctaaggacg ccatccgttt gggcgggttg ataacgggca aaggatggta taccaaggaa
      781 ttacaggcgg aatatatcaa aaaggccact gaaaataggg gtataggatg agccggcctt
      841 ttcggaatga aacgggaaaa catatacttg gtttctatgg gctctaagtg cattcctatg
      901 aaacccgacc gaagccggat aaagattgat cttggcagcg tttaatggta atatggttgc
      961 aaaccgaatt ctttaactat caaagaacat accattccaa agtggcttgc acagttttat
     1021 catggtatta cttatgttat tgtcaaagga gtctaacaat ggatcagctt ttattcggta
     1081 tttccggact tccggtaggg gatcagtctc caaaacgaaa ttatcgcagc ggtatcgctt
     1141 atttgaagac ggcggggttg gacgccatgg aacttccctt tgtaaggaat gtgaacgtaa
     1201 ccgataaaaa caaggatgaa atccttcagg aaaaactgga caaggatttc ttcctctcgg
     1261 cgcacggttc ctattttatc aatctcaacg ccgatgaaac ggaaaaacag caacagtcca
     1321 tggaacgaat cattaaaggg gccaaggcgt tgaaatccgt cggcgggaac agcctcgtct
     1381 ttcatccggg gttttatctg aacgattccc ccgaggttac ctatgccacc atcaaggaga
     1441 acttgatgaa actgccggat ctgggcgtag cgtaccggct cgagaccacc ggaaagccga
     1501 cccagttcgg cagtctcgat gagctgattg gcttatgcaa cgaagttccc acctgccggc
     1561 tttgcgtcga tttcgcgcat attcatgccc ggggcggcgg atctcttaaa acctatgacg
     1621 attataccgc catcttggca aagatccggg agcgtctggg tgaagcggcc ctgttcgaca
     1681 tgcacattca tctctccggc atcaattacg gcgccaaggg tgagaagaat catttgagtt
     1741 ttgcggagag cgatttcaac tacaccgact gcctgaaggc actgaaggat tataaagtca
     1801 agggttgtgt gatctgtgaa agcccgatcc tggagaagga cgccgcgctt ttgaaggaga
     1861 cctataaccg gctttgaatt gaaaactgtt ccacaacaaa acaaccccgg tttaatccgg
     1921 ggctttttat actataaact tatgagtcat gttccataaa cattaaattt cgttgaatcg
     1981 aaaagctgtt gctttgatca aaccgcctcg agcaactggt acatcttctc ggactcgtca
     2041 tgttccagtg tctcttcgat ataggtctgg gtgtactcct cgtccgccag gttcagccga
     2101 atgggctgct ccaacttttg ttgggtgcga tccaaaagga tccggatctc cggacggaac
     2161 gggtacccgt ggttgatccc gatcaccttg gccacttcgc cggtattcaa ccggaccaga
     2221 ctgccgatgg gataaatcgc catactggtt actaacgatt ttaatacccg gatatcaaag
     2281 aggcgcgact cccccagcaa gttcttcaac gcttcgtaag gggtcatccg cggccggtat
     2341 ttacggggag agatgcaggc gtcataaacg tcggctacgg cgataatccg gccgaagaga
     2401 tgaatctcct cgcctttcaa tccccgtgga taaccactgc cgtc
//
LOCUS       DASVMB010000103         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_601114,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000103 DASVMB010000000
VERSION     DASVMB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601114"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VF531_03330"
     CDS             <1..730
                     /locus_tag="VF531_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7713030.1"
                     /translation="IIAKMGSISVSELSRQLGVSVVTIRKDLEVLESDNSVTRTYGGV
                     IHGSKPIQEEIPELPKFSAQVIEAAGFIAPGQTIMLGGGKHIVSLADYIKNYENLTVI
                     TISIEVALTLMKAPHIDVILTGGILRARTMTMLGFLTERVIKEIAFDISFSEADGIHP
                     QNGITSANMVEASTESTLLKYSNRPIILAEAGAIGRSAPVQISKLKPELTIILEGELE
                     RNTLKELESSGTQVIIPATASNHP"
     gene            1009..2298
                     /locus_tag="VF531_03335"
     CDS             1009..2298
                     /locus_tag="VF531_03335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7713031.1"
                     /translation="MHSLMLSWEFPPKIVGGIARHVYDLSLALLKQGVDVTVITCDMQ
                     GAAKYEEIHGIKVHRVPMSNPSPPDFLTWVLQLNLNMIEQANRLAFAGTTFDVIHAHD
                     WLAGFAGKNLKHTWKNPLVATIHATEFGRNNGLYNELQRYISNVEWWLGYESWRVICC
                     SLFMRDELQRVFQMPDDKIRVIPNGVYPEEFAKTDLDPAKIRNQYCAPDEKIIFYVGR
                     IVREKGLGVLLDAFSRVLVVESKAKLVIAGTGPYMDELKHRAYCLGIFNRIYFTGYID
                     DNTRNALYKSAAVAVFPSLYEPFGIVALEGMAAGTPVVVSDAGGMSEIIEHGVNGLKA
                     YCNNASSLADNIIWVLQHPDHAGKIRERALNDIQKIYNWDRIAAQTIDVYREILDEYH
                     ESNWRAVMHEEAARDMFMSRMVQSIGAEEMSRYRDVH"
     gene            2323..>2657
                     /locus_tag="VF531_03340"
     CDS             2323..>2657
                     /locus_tag="VF531_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7713032.1"
                     /translation="MKAVIMAGGKGTRLRPLTCSLPKPMVPIANRPMMEYIVRLLKEH
                     GFTEVLATLFYLPEAIQNHFGDGHDFGMEIRYLLENNPLGTAGSVKNGEDFLDETFLV
                     ISGDTLTDI"
BASE COUNT          697 a          659 c          674 g          627 t
ORIGIN      
        1 gatcatcgcc aaaatgggca gtatctctgt ctccgaatta agccggcaac tcggggtttc
       61 agtggtaacc atccgtaagg atttagaggt tctggagagc gataactcgg tcacccggac
      121 ctacggcggg gttatccacg gcagtaaacc cattcaggaa gagattcccg agctccctaa
      181 attttcggct caggtgattg aagctgccgg tttcatcgct cccggccaaa ctatcatgtt
      241 gggcggaggc aaacatattg taagtcttgc ggactacatc aagaattatg aaaacctcac
      301 cgtaatcacc atctcgatag aagtggcgct gaccttaatg aaagccccac acattgatgt
      361 aattttgact ggtggaattt tgcgggcccg gaccatgacg atgttgggat ttctcacgga
      421 gcgggtcatc aaagaaatcg ccttcgacat ctctttttcg gaagctgacg gtatccatcc
      481 ccaaaatggg atcaccagcg ccaatatggt agaggcaagt actgaatcga cactattgaa
      541 atacagcaat cgcccgatca ttctggctga ggccggagcg atcggccggt cggccccggt
      601 tcagatatcc aaacttaagc cggagttaac cattatcttg gagggagagt tggaacggaa
      661 taccctaaaa gagctggaat caagtggaac ccaagtgatt atacccgcca cggcatccaa
      721 tcatccctaa aaacacctgc taaatcaata tcccccggct atgccggggg atattgattt
      781 gaaatccctt ccaaaaactg aaactccccc ctaaataggt ttaatatctt atttcactgg
      841 ataaactatt tctcatccaa tggttggcaa ttaatcaccc acgctctcgc aacgacacct
      901 acaaatggga tttcaggagg acccatgaga aaagatgaga agcaatagtt tttcactatt
      961 gcctgttgcc tattgcccgt tgccaattta tcaaaccgag gtgaaacaat gcacagtttg
     1021 atgctaagct gggagttccc tcccaagatc gtcgggggta ttgcgcgtca tgtttatgat
     1081 ctctccctgg cactgctaaa acagggggtc gatgtgacgg taatcacctg cgacatgcag
     1141 ggtgccgcga aatacgaaga aatacacggg atcaaggttc accgggttcc aatgagtaat
     1201 ccgtcaccgc ctgacttttt aacctgggtg ttgcaactca atttaaacat gattgaacaa
     1261 gccaatcggt tggccttcgc cggaaccacc tttgatgtga ttcatgccca tgattggttg
     1321 gcgggattcg ccggaaaaaa tctcaaacac acctggaaga accccctggt ggcaacgatt
     1381 cacgccaccg agttcgggcg caacaacggg ctatacaatg agctgcagcg ttatatcagt
     1441 aacgtggaat ggtggttggg atacgagtcg tggcgggtca tctgctgcag cctttttatg
     1501 cgggatgaac tgcaacgggt ctttcagatg ccggacgaca aaatccgggt gatccccaac
     1561 ggggtttacc ccgaggagtt cgccaaaacc gaccttgatc cggcaaagat tcggaatcag
     1621 tattgcgcac ccgatgagaa gatcattttc tatgttggcc ggatcgtccg tgagaaaggc
     1681 ctgggagtgt tgctcgatgc cttctcaaga gtactggttg tggaatccaa ggccaaactg
     1741 gtcatcgccg gaaccggtcc ttatatggat gaattaaaac accgcgccta ctgcctggga
     1801 atcttcaacc ggatctattt taccggctac atcgatgata atacccgtaa tgccctttac
     1861 aaatcggctg cagtagcggt cttccccagc ctgtatgaac ccttcggcat cgtggccctc
     1921 gaaggtatgg cagccggtac tccggtagtt gtctcggacg ccggtggaat gagcgagatc
     1981 atcgagcacg gcgtcaacgg acttaaggcc tattgcaaca atgccagttc tctggctgac
     2041 aacatcatct gggtattgca acatcccgac catgccggga aaatccggga gcgggcgctt
     2101 aacgatattc agaagatcta taactgggat cggatcgctg cccagaccat cgacgtttac
     2161 cgcgagatct tggatgagta ccatgaatca aattggcggg cagtgatgca tgaagaagcc
     2221 gcccgtgaca tgtttatgtc gcgaatggtc caaagcatcg gcgccgaaga gatgtcccgc
     2281 taccgggatg tgcattgatt tgataaggag gcatcctttc agatgaaagc agtaatcatg
     2341 gccggcggta agggaacccg gttgcgacct ctcacttgta gcttaccaaa acccatggtc
     2401 cccatagcta accggccgat gatggagtat attgtgagac tgttaaaaga gcacggtttc
     2461 accgaggtac tggccacctt gttttacttg ccggaggcaa tccaaaacca tttcggagac
     2521 ggtcatgatt tcggcatgga gatccgttac ctgttagaaa ataatccgtt ggggacagcc
     2581 ggaagtgtaa aaaacggtga ggatttcctg gatgaaacct ttttagtgat cagcggcgac
     2641 accctgaccg atattca
//
LOCUS       DASVMB010000104         8611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_529267,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000104 DASVMB010000000
VERSION     DASVMB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529267"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VF531_03345"
     CDS             complement(<1..1366)
                     /locus_tag="VF531_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006040370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX7713033.1"
                     /translation="MADDVVGFVKADGERLINGEGREILLRGVGFGGWLLPEGYMWRF
                     PKQGDRPRRIERMVAGLVGPVQAARFWDSYYERYIAEADIRQIAAGGFNSVRVPIPYR
                     FLIEGDGSGEATFGKNGAIRYQERHLELLDRLIGWCRASRLYVILDLHAAPGGQTGTN
                     IDDSERDQPELFTDKRNQSLTVAIWRMLAGRYRDEWIVAGYDLLNEPLPEWFSTYNPK
                     VMPLYREIIKAIREVDSNHLIILEGVHWATDWSIFSEASARKIDGNLMLQFHKYWNNP
                     DTESIRVYLDKRREWEIPIFMGEGGENNKDWYAGTFQMLEEHNVSWNFWTWKKMDTDN
                     SPCSVAQPPDWQKLTDYLEGGPKPDQETAARVLGEYLDNLPFERCHYHPEVADSLFRR
                     PPLRIPAVFYGYRGPGVSFGIKNRSELHHEFRMHDGTDIRFVQGTRSTPNFQHGRGEP
                     WQEDE"
     gene            complement(1397..2176)
                     /locus_tag="VF531_03350"
     CDS             complement(1397..2176)
                     /locus_tag="VF531_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713034.1"
                     /translation="MAAFALFPIYMALLNSLKTQGEMLTDILAFPSHLAFTNYAEAFR
                     KTNFLHSLLNTIFVVVIGVTGMIIFSSMAGYKLSRTPGWLSRFLFGLFVMSMLIPFHS
                     IMITLTKIARDLSIQGSAPGLGLIYIGLGCPMAIFMYHGFVKSIPRELDEAALIDGCG
                     ELRLFFSVVFPLLRPITATIAILNTLWIWNDFLLPQLMLTDFDQYTLLLSTNALFGEY
                     GNNDWSAILATLIMAILPVIMIYLVLQKYIMKGIVEGAIKG"
     gene            complement(2272..3153)
                     /locus_tag="VF531_03355"
     CDS             complement(2272..3153)
                     /locus_tag="VF531_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713035.1"
                     /translation="MTRKRKQTVTLLLFVLPALLFYAAFMLASTFGGVWYSLTNWNGL
                     NPTYKMVGFANYIEALTEDPDFLKSILFTLKYVIFMVILQNVIALLLAVLVESRRRSK
                     VGFRTIFFMPNMISMIIGGFMWMFIFTKVLSFIGQHTVLHFLDHSWIGDPTYSFYAIL
                     TVSLWGGVGYLMVIYIAALQGVPQQLKEVALIDGANGFQAFRYVSLPMIFPALTIGIF
                     VTLNSSFKVYDAVYALTGGGPGRATQVIALNIVEEAFKMNNRYGYASAKAIILFLIVF
                     FITLGQLWMMKRREVEV"
     gene            complement(3183..4439)
                     /locus_tag="VF531_03360"
     CDS             complement(3183..4439)
                     /locus_tag="VF531_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713036.1"
                     /translation="MKKVVSLVMILALILAVGAGSTLFAKAPAKQVELKVFMSFPRFK
                     EQFDKYFEQFKAKEKAEKNVDVTIKLEMPSSDQAGQILKARLASNDAPDLFTLHAIAD
                     IPTFYKAGYLTDLSRQPFVGSLYGNVKKIVTYDRKVVALPLESLAWGYLYNKDIFNKY
                     GLTPPQTLDEMKKVVGKLKANNVTPFLLSFQESWIPQLMMALSLGGVVTSERPDFVTK
                     MNQGKASYKDVQNVFNIIDLIMQNGTDKPFEVGSAAGSADFANGKAAMWVQGPWQAEA
                     IFSVNPNMKFGVAPLPVSNNPKGTLINLSVSTSVAVSPTSKNKQVAFDLLNYLLDKKD
                     SSALFQELKFNPLSSVHKYQSYPWVDEAMKYVSQGKSYQDLSLPQAVTDETAKMLQSY
                     YAKSVTKEEMIKALDRAWATANKVNN"
     gene            complement(4567..5673)
                     /locus_tag="VF531_03365"
     CDS             complement(4567..5673)
                     /locus_tag="VF531_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713037.1"
                     /translation="MCHACAKVLIIDDEPWSREVVKSLGAWQSLQLEVIGEAEDGTEG
                     LRLIVELKPDIVVTDMRMPGIDGVELLREMHERFPSLKIIVMSGFDDFVYLKQAIRSR
                     VVEYLMKPIDPEELNASLARCSQELIEDQGSANPLDGSALLFTDPAVLDEYLALRRKV
                     YGYLLELNGASIPDAFAKLERFLQSALREEQDGNMLSQISHDFLLMLEEFAAENQISR
                     ELLKNDPFHGGGFVPGTDALSGVIDELRRLYLGAVDAVAKLSRSRNGLDLAEVQAYIE
                     QHFQDQISLETIARRFFISKEHLSRAFKTFSGENISDYITRKRMEKARDMILGQGLEI
                     KRAAQLTGYEDLAYFYRVFKKHFGLTPGELRRED"
     gene            complement(5639..7441)
                     /locus_tag="VF531_03370"
     CDS             complement(5639..7441)
                     /locus_tag="VF531_03370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7713038.1"
                     /translation="MKRTSIRFRLMVLMICLTTLPVITVTWIATNDTRRSVEKEIINA
                     NDSRMIWADQYLNDLIQRIDSLFYSLQINQSLMAALDEMDRQDRGAQFRTQIYIRDTL
                     TAAFLANSQKVDELTLYFHSIQKTFSVNFATSGKISVIDIRNGPWNRMQHSPAYMYFK
                     QSGDDIYAFHSINRFEDRKLLGGLSVRINRKVWDEVSAILRSEPESSVFLMNDEGEML
                     SGSTVLKSFNAIRAQIHKLDSENPGVIFNRTKDYLYFMKRIGDGQLTIVKAIPVKTIT
                     RSARSTIQAGILTGTLFAVISILLSILVSLRISGPIVSLARTMKVVKINDFEMKSVQN
                     RDEIGQLETAYNSMMQRIKELIEEEYQKNIELKNAQLMAMQAQINPHFLNNTLNLIGG
                     MALSKKVPEIYRITRVIGNLLRYSISTTDDIVTLEEELKHMRNYLFIQEQRFAGRCTV
                     AVTVDETVLDSRLPKFTLQPVVENAFEHGLHGKEGAWKVEIRVRRIRSRICLMVKDQG
                     VGIPTEQLRRLRAELQGGPAQKESRKPEDGPKKRKGIGLRNVAARLKLQFGETYGVRI
                     FSRPGVGTLVLMVLPVFQQEGGADVSRVREGINH"
     gene            complement(7956..>8611)
                     /locus_tag="VF531_03375"
     CDS             complement(7956..>8611)
                     /locus_tag="VF531_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7713039.1"
                     /translation="MALQFTLASNVGLLVATAPLLTAVIAHYFVRGERLKRGVVFGFL
                     LAVLGVFLVMGNGRFVVKVNPKGDFLALGAALLWAVYTILLKRVDSGYHPIFVVRKTF
                     FYGLITMLPLVMILPGSVKTVHSFGGPMIGNILFLGLAASALCFVLWNRAIRTLGAVR
                     VTNYIYLVPLITMVTSVIVLHEPVTGFMVAGACLICGGVYLSEHELSSKSGQEIKAV"
BASE COUNT         2158 a         2349 c         2025 g         2079 t
ORIGIN      
        1 actcatcctc ctgccagggt tcaccccgtc cgtgttgaaa attgggagta ctccgcgttc
       61 cttggacaaa acggatatcc gttccgtcgt gcatccgaaa ctcatggtgc aattccgagc
      121 ggttcttgat tccgaaactt acgcccggcc cccggtatcc atagaaaacc gccggtatcc
      181 gcagaggtgg cctgcggaag agtgagtccg ccacttccgg atggtaatga catcgctcga
      241 aaggcaggtt atcgagatac tcccctaaga cgcgagccgc ggtttcctga tccggtttcg
      301 gtccgccttc taagtaatca gtgagctttt gccagtccgg gggttgggcg accgaacaag
      361 gcgaattatc ggtgtccatc tttttccacg tccaaaaatt ccacgaaaca ttatgttcct
      421 cgagcatttg gaatgtgccg gcataccaat ccttgttatt ctcgccgccc tcgcccatga
      481 agatcgggat ctcccattcc cggcgcttgt ccaaatatac ccggatgctc tcggtatcgg
      541 gattattcca atatttatga aattgaagca tcagattgcc gtcgatcttc cgggcgcttg
      601 cttcactaaa gatcgaccag tccgttgccc aatgaactcc ttccaggatg atcaggtggt
      661 ttgaatccac ctcccggatt gccttgatga tctcccggta taacggcatc accttggggt
      721 tatatgtcga aaaccattcg ggtaacggct cgttcaacag atcatagccg gctacaatcc
      781 actcatcccg gtaacgtccg gccaacattc gccagatcgc gaccgtaaga gattgattgc
      841 gcttatcggt aaagagttcc ggctgatccc tctcggaatc gtcaatgttt gttccggtct
      901 gtccaccggg cgccgcgtgc aaatctagaa tcacatagag gcgactggca cggcaccagc
      961 cgatcaagcg gtcgagcagt tccagatgac gctcttgata acggatagcc ccattctttc
     1021 caaacgtggc ttctccggag ccatcccctt cgatcaggaa ccggtaaggg atcggtaccc
     1081 ggactgaatt gaaaccgccg gctgcgattt gacggatatc cgcttcagcg atataccgtt
     1141 cataatagct gtcccaaaac cgtgccgcct gcaccgggcc gactagaccg gcaaccatcc
     1201 tttcgatgcg ccgcggccga tctccctgtt tgggaaaacg ccacatatat ccttccggaa
     1261 gcaaccagcc gccgaaaccg acaccccgga gcaaaatttc acgtccttcc ccgttgatca
     1321 atctctcgcc atcagccttt acaaatccaa ccacatcatc cgccatgttc acgccatccc
     1381 ccaaaaattt attttcttat cccttgatgg ccccttccac gatacccttc atgatgtact
     1441 tctgcaatac cagatagatc atgatgaccg gcaagatcgc catgatcagg gtggccagga
     1501 tcgccgacca atcgttgttg ccgtactcgc cgaaaagcgc attggtcgat agcaacaggg
     1561 tatattgatc aaaatccgtc aacatcagct ggggcagtaa aaagtcgttc cagatccata
     1621 acgtatttag aatggcgatg gttgcggtga tcgggcgcaa taacggaaag actacggaaa
     1681 agaacaatct caactcgccg cacccgtcga ttagtgcggc ttcatccagt tcccgcggga
     1741 tggatttgac gaacccgtga tacataaaga tcgccatcgg acatcccaga ccgatataga
     1801 tcaaacccag gcccggcgcg gaaccctgaa tcgagagatc ccgggcgatc ttggtcaaag
     1861 tgatcatgat tgaatgaaac gggatcagca tcgacattac gaacaggcca aataaaaaac
     1921 ggctcagcca acccggggta cgggacaatt tgtagccggc catcgacgag aagatgatca
     1981 tcccggtcac gccgatgact accacgaaga tggtgttaag cagactatgc aaaaaattgg
     2041 ttttccgaaa agcctcggca taattggtga atgccaaatg ggacggaaaa gccaggatat
     2101 ccgtgagcat ctcgccctgg gtcttcaggg agttcaacaa ggccatataa atggggaaca
     2161 gggcaaaggc cgccataacc aacagaaata gaaaaatgag cgcggagagg attcggcctt
     2221 cgggagcccc gcgccgggac ttcttttcaa acttcttgat atcaaactcc ctcatacttc
     2281 cacctctctt cgcttcatca tccaaagctg gccgagggta atgaaaaaga cgatgaggaa
     2341 caggataatc gccttggcgc tggcatagcc gtaacggtta ttcattttaa aagcttcttc
     2401 cacaatatta agcgctataa cttgggtcgc acggccggga ccgccgcccg tcagtgcata
     2461 aacggcgtca tagactttaa atgaggagtt caaggtgaca aaaatcccga tggtcaacgc
     2521 cgggaagatc atcggcagcg aaacataccg gaaggcttgg aacccattgg ccccgtcgat
     2581 caatgcaacc tctttcagct gctgtggtac tccctgaagc gccgcgatat agatcaccat
     2641 cagataaccg actccgcccc agagtgagac cgtaaggatc gcatagaatg aataagtcgg
     2701 atcaccgatc caggaatggt ccagaaaatg aaggaccgtg tgttggccaa tgaacgacag
     2761 gaccttggtg aagatgaaca tccacataaa tccgccaatg atcatgctga tcatgttggg
     2821 catgaagaat atggtacgaa atcccacctt gctccggcgc cgcgattcaa ccaagaccgc
     2881 caggagcaag gcaatcacgt tctggagtat caccatgaag attacatact tgagggtaaa
     2941 caaaatcgat ttcagaaaat cggggtcctc cgtcagagct tcgatataat tggcgaatcc
     3001 caccattttg taggtgggat tcagaccatt ccaattcgtt agactatacc ataccccgcc
     3061 gaaagtggag gcgagcataa aggcggcata gaacaacagc gccggaagca cgaagagtaa
     3121 cagagtgacc gtttgtttcc gcttgcgagt cattacagtc acttccctgt ctgaggttgg
     3181 atttaattat taaccttatt tgccgtggcc catgccctat ccagcgcttt aatcatttct
     3241 tcctttgtca cgctctttgc ataataactt tgaagcattt tggcggtttc atcggttacc
     3301 gcctggggta acgacagatc ttgataggac ttgccctggg atacgtattt catggcctcg
     3361 tcaacccatg gataggactg gtacttatgg accgacgata acggattgaa cttcaactcc
     3421 tggaataaag cggaggaatc cttcttatcc agcagatagt tgaggagatc gaacgccact
     3481 tgcttatttt tgctggtcgg agagaccgcc accgaagtgg ataccgacag gttgattaac
     3541 gttcccttgg ggttattgct caccggcaac ggcgccacac cgaatttcat attcgggttg
     3601 accgagaaga tcgcttccgc ctgccacgga ccctgtaccc acatggcagc cttgccgttg
     3661 gcgaaatcgg ccgaacccgc cgcgctccca acctcgaacg gtttatcggt gccgttttgc
     3721 atgatcaggt cgatgatatt gaagacattc tgcacatctt tatacgaagc cttgccttgg
     3781 ttcattttgg ttacgaaatc cgggcgttcc gaggtaacca cgccgccgag ggataacgcc
     3841 atcatcagct gcgggatcca ggactcctgg aaggaaagaa gaaacggcgt gacgttattg
     3901 gccttcagct tcccgaccac cttcttcatt tcatccaatg tttgcggcgg ggtgagacca
     3961 tatttattga aaatatcctt gttgtacaga tacccccatg ccagactctc caggggcagg
     4021 gctacgactt tgcggtcata ggtgacgatt ttcttgacat tgccgtataa acttccgaca
     4081 aacggctggc gggagaggtc agtcaggtaa ccggccttgt agaaagtggg aatatcggcg
     4141 atggcgtgca gggtaaacag gtccggcgca tcattggagg ccaggcgggc tttgagtatc
     4201 tgaccggcct gatcggaact gggcatctca agcttaatcg tgacatcgac gttcttttcc
     4261 gctttctcct tcgccttgaa ctgctcgaag tatttatcga attgctcttt aaacctgggg
     4321 aagctcataa agactttcaa ttccacctgc ttggccgggg ccttggcgaa cagggtgctc
     4381 cctgccccga ctgccagaat caaagcgagg atcatgacca atgagaccac ttttttcatt
     4441 tttctttccc tcctaaaatg aacttattgt aatgcttgtc tagatcaaag tatatcccat
     4501 acggtcccgt gaatcattgc aggatattag cttcaactat tgcactttat tgagatactt
     4561 taaatatcaa tcctcgcgcc gcaattcacc gggcgtcaga ccgaaatgtt tcttaaaaac
     4621 ccggtaaaaa taagccagat cttcataccc cgtcaattgt gccgcccgtt taatctccag
     4681 cccttgcccc aatatcatgt ctctagcctt ctccatccgt ttccgtgtga tataatccga
     4741 gatattttcg ccgctaaagg ttttaaacgc ccggctcagg tgttccttgc tgatgaagaa
     4801 gcgccgcgca atcgtttcta gtgagatctg gtcttgaaaa tgttgttcga tatatgcctg
     4861 gacttctgct agatcaaggc cattccggct ccggctaagc tttgcaaccg catcaaccgc
     4921 tcccaggtaa agccggcgaa gttcatcgat cactcccgaa agggcatcgg taccgggtac
     4981 gaaacctcct ccgtgaaatg gatcgttctt caggagttcg cggctgatct ggttctcagc
     5041 tgcgaactct tccaacatca gcagaaaatc gtgactgatt tgactcagca tgtttccgtc
     5101 ttgctcctcc cgaagcgcgc tttgtaaaaa acgctccagt ttcgcgaagg cgtccggaat
     5161 ggatgctccg ttcaactcca ataagtaccc gtagactttg cgtcgtaagg ccagatattc
     5221 atcaaggacg gcgggatcgg taaaaagcaa cgccgaaccg tctaaaggat tagcggatcc
     5281 ctggtcctca ataagttcct gactgcaccg cgccagggaa gcgttcaact cctccgggtc
     5341 gatgggtttc atgagatatt ccaccacccg ggaacgaatc gcctgtttca aatagacaaa
     5401 atcgtcaaag ccgctcataa caatgatctt tagcgacgga aaacgctcgt gcatctccct
     5461 taggagctcg actccgtcaa tgccgggcat acgcatatcg gtcactacga tatccggttt
     5521 caactccacg ataagcctga gtccctccgt accatcctcc gcctcgccga tgacctccag
     5581 ttgcagactc tgccatgctc cgagcgactt gaccacttcc ctggaccagg gttcatcatc
     5641 aatgattaat accttcgcgc acgcgtgaca catccgctcc accctcctgt tggaatacag
     5701 gcagcaccat cagaaccaag gtgccgactc ccggccggct gaagatccgg accccgtatg
     5761 tttctccaaa ctgcagcttt agccgggccg caacatttct caaaccgatg cccttccgtt
     5821 ttttcggtcc atcctccggt ttccgggact ccttttgcgc cggaccgccc tgcaattctg
     5881 cccggagccg ccgcaactgt tccgtaggga ttccgacccc ttgatccttg accatcaaac
     5941 agatccggct tctgattcgc cgcacccgaa tctccacctt ccaagccccc tctttgccat
     6001 ggagtccgtg ctcaaaagca ttttcaacca cgggttggag tgtaaacttc ggtagcctgg
     6061 agtcaagcac tgtttcatcg accgtaaccg caaccgtgca gcgtccggca aatcgctgct
     6121 cctggatgaa aagatagtta cgcatatgtt tcaactcttc ttccagggtc acgatatcat
     6181 ccgtggtact gatggaatat cgtaatagat tcccaatgac ccgggtaatc ctgtatattt
     6241 ccggtacctt ttttgaaagc gccatccccc cgatgaggtt cagagtatta ttgagaaaat
     6301 ggggattaat ctgggcttgc atggccatta gctgagcatt ttttaattcg atattctttt
     6361 gatactcctc ctcgatcaac tctttaatcc gttgcatcat ggagttgtat gccgtctcca
     6421 attgcccgat ctcgtcgcgg ttttgcaccg atttcatctc aaagtcattg atctttacca
     6481 ctttcattgt ccgcgccagg ctcacaatcg gtccgctgat ccggagtgaa accagaatgg
     6541 agagcagaat tgaaatcacc gcaaacaagg ttccggtcaa gataccggcc tggatcgtcg
     6601 aacgggcgct acgggtgatg gtcttgaccg ggatagcctt aacaatcgtc agttggccgt
     6661 cgccgatccg tttcatgaaa taaaggtaat ccttggtgcg gttaaatatc actccggggt
     6721 tttctgaatc aagtttgtga atctgcgccc ggatagcgtt aaaactcttc agtacggtag
     6781 aaccggataa catctcccct tcatcgttca tcagaaagac ggaactttcc ggctccgatc
     6841 tcaaaatggc gctcacttca tcccagacct tccggttgat tcggaccgat aaacccccga
     6901 gtaactttcg atcctcaaaa cggttgatac tgtggaatgc gtagatgtca tccccggatt
     6961 gtttgaagta catataggcc gggctgtgtt gcatccggtt ccaaggcccg ttacggatat
     7021 cgatgacgga tatcttgccg ctggtcgcaa aattgactga aaatgtcttc tgaatcgaat
     7081 gaaagtagag cgtcaactcg tccacttttt gggaattggc caggaatgcc gcggtgagtg
     7141 tatcacggat ataaatctgg gtcctgaatt gggcgccccg gtcctgcctg tccatttcat
     7201 ccaaggccgc catcagcgat tgattgatct gcaatgaata aaacaagctg tcaatccgtt
     7261 gaatcagatc gttcagatac tgatcggccc agatcatacg ggaatcgttg gcattgatga
     7321 tctccttctc caccgaacgc cgggtatcat tggtggcgat ccacgtcacc gttatgaccg
     7381 gcagtgttgt cagacagatc atcagcacca tcaaacgaaa gcggatgctg gttcgcttca
     7441 tcctatctat cccttctaac atccgtgctc aaggaaattg ccgtgtccgg catcaaagaa
     7501 aggcggttca aaacggcttt gattcgatat cacaattata ccaggaacta taaagtcctg
     7561 ctcctgcttt tcatggataa acaagttgtt tccacagctt tccggccgtt tcagctcaat
     7621 aaaaaaagtc ccgaccaggg acttttttac agactaaatc atcagtgaag gccggtatgc
     7681 ggcaaaagcg gttatctcag gagtttcaaa ctcccggtta attgattgat cgtcttctcc
     7741 ggcccatata agccgatgct caaatagtcc aactcctccc cgttcaattg taatagagca
     7801 tgggcatagt cagaatggca tttgcactgc tgggctgcgg tgctaaagtc aatcgtctgt
     7861 acttcggcat actccgcccg gtaaagttga ttgcgcagat tgcggatata ttcaccattt
     7921 gcgctcagga tgggaatgga gactctcgct gttttttaga ccgcttttat ttcctgcccg
     7981 gacttcgatg atagttcgtg ttcggagagg taaactccac cacagattag gcaagcgccc
     8041 gccaccataa aaccggtcac cggttcatgg aggacgatga ccgaggttac catggtaatc
     8101 aaaggcacca gataaatgta attggtgacc cgcaccgccc ccaaagtccg aatcgcccgg
     8161 ttccacagga cgaagcatag cgcggaagcg gccagcccca gaaacaatat atttccaatc
     8221 atcggcccgc caaaggaatg aacggtttta acgcttcccg ggagtatcat gacgagtggc
     8281 agcatggtta tgagcccgta gaaaaaggtc ttgcgtacca caaagatagg atgatacccg
     8341 gaatcgactc ttttgaggag tatggtgtaa acagcccaga gaagcgccgc tcccaacgcc
     8401 agaaaatcgc ctttgggatt tacctttacg acaaaccggc cgttgcccat gaccagaaat
     8461 actcccagca cggcgaggag aaatccgaaa accacacccc gtttcaatcg ctctccccgg
     8521 acaaaataat gagcgataac cgcggtcaaa agaggcgcag tcgccaccag caaacccaca
     8581 ttggacgcaa gagtaaattg caaagccata t
//
LOCUS       DASVMB010000105         5664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_235852,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000105 DASVMB010000000
VERSION     DASVMB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235852"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VF531_03380"
     CDS             <1..626
                     /locus_tag="VF531_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014651941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX7713040.1"
                     /translation="QFFEVALCFSVINMAFAIAECLARGFDAFSALVSSGDFDRLLLR
                     PRNTVVQVMGSKFEFTRVGRLLQSMIVLIWAITNLPVHWNLLKAITLILMIASGVSIF
                     TGIFILAATMCFWTVQSIEVANIFTDGGREISQYPLNIYQKWVTRFFTFVIPFGCMNY
                     LPLLYILDRVKGNGVLYMLTPLYGILFVVPCLWVWRYGVRHYRSTGS"
     gene            1708..4614
                     /locus_tag="VF531_03385"
     CDS             1708..4614
                     /locus_tag="VF531_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7713041.1"
                     /translation="MRTAGGGINCCIVPLNGGQIDISKMNEVFTGDGLEDIPRFDDRH
                     FYWSKDGKFIYGLADGKVMVLDAVNRSFRYWDHQADDLNQPFSPEGCYFITKQGNRVQ
                     LHSLAEMISEITLDFEPKFSYWLPSVQEPRFVLADANHVVLYHNSVEMQRFDTKSNIQ
                     AIEVAKDGQNAVLLCDTCIEVWSLAHTPVCRQIIQSDHVMKDIRLGADNMLVVFCNDR
                     LDDLNEDEANVLEFWDLNADEKIAEHLIRGLYESEDELEDLMEELDNGDQEIGAVMIK
                     RDKRGYWGAVVKADRFVLPSKKLKKDLSLLNPYLSFVIEKRWSWPWHWANGTQHIEVG
                     SKKVKKDEYAEERELLRQKMEDISNGGEIDAAIDDTGYTALMVAAKIGDIDLMKQLIE
                     TKADVNAKTKSGTTALMLACNCGHTEAVRLLIEAGANVHHKNEYEETALGWAVDPDKP
                     EIIKLLIDAKADFQAKDDQGYTPLIVAAAKGCTEIVKFLLEKQVEIDARNYNGDTALI
                     LAAKQGCTKAVKLLIEAKADLDAKNNDGNTALILAAGYKYLEIEKLLIHGGSNPYEKN
                     NEGKTAYNDDLLILILFDAVTKNSPESIKSYIKNIGKLDITDDQGLTALMLGAQLGQM
                     EAVKTLIVARAKMDLTNKKGETALMLAENNDHHDVAELLKREAAVGQAVDRFFDAIKE
                     NDLELITSYLHSGYDVNRKNQEGCPALVWAAQNGQAAVVKLLIEAKAEVNATFYKSIY
                     GDRMTALMAAGDDAEITKVLLDSGADVHLKDDKGKTALIWAVIKNNLEVIKLLIAGHA
                     DVDATDKYGESTFLIAAFKGNVEILKFLSTAGADIHLKNSRGETALIQATDRGNIEAV
                     KHLIEAGLDINAKNKDGESALFLAANNNYPAILELLINAGAEISVYDGEGENVLIKAV
                     QKRYIEVVKILIKAKVDVNAKDRFDRTALSIANGQEDSEMIKLLLEAGADTTNA"
     gene            4683..5486
                     /locus_tag="VF531_03390"
     CDS             4683..5486
                     /locus_tag="VF531_03390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713042.1"
                     /translation="MVKQAVSSQIEFIVDLDETVIGSRCRESLIRQAVGNGECNVVLK
                     EMTIMGFGILDYSFFENGFIKLLIVGSGWRRRGFGRLLVETFKEQCRTEKLFTSTNRS
                     NRPMQMLLAGCGFERCGLVERLDEGDPELIYCFERNRRTLASPKSQALTGFDDKFWGA
                     VDVLVAGAELVIDRPKGSCHPRFPEVIYPVDYGYLKGTTAMDGGGIDLWSGTGKDRRV
                     AAIICTVDLNKKDSEIKLLFGCTAAEQELIYRFHNESEYMKGILIPRLS"
     gene            5483..>5664
                     /locus_tag="VF531_03395"
     CDS             5483..>5664
                     /locus_tag="VF531_03395"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002021167.1"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7713043.1"
                     /translation="MKGSVRLFNVVEASIADLQAAMGAGQVTSRELILMYMERIAKYD
                     KDGPKLNAILEINPDA"
BASE COUNT         1625 a         1035 c         1513 g         1491 t
ORIGIN      
        1 ggcagttctt tgaggtggcc ttgtgcttct ccgtgattaa catggctttt gcaattgcgg
       61 aatgtttggc cagaggtttt gacgcttttt cggccttggt ttccagcgga gatttcgata
      121 ggttgctgtt gcgacccagg aatacagtag tgcaagttat gggatcaaaa tttgaattca
      181 ccagggttgg caggcttttg cagagcatga tcgttttaat ctgggccata actaatcttc
      241 cggttcattg gaatctcctc aaggccatta ccttgatctt gatgatcgcc agcggtgtct
      301 caatatttac gggcattttc attttggcgg ccaccatgtg tttctggacg gttcaaagta
      361 tcgaagtggc caatattttt accgacggcg gccgggagat atcccagtat ccgttgaata
      421 tctatcaaaa gtgggtgacg cgttttttca ccttcgtgat tcctttcgga tgtatgaact
      481 acttgccgtt attgtacatt ctggataggg tcaaaggaaa cggggtcctg tatatgctga
      541 cgccgttgta tgggatactg tttgtcgtgc cgtgcctgtg ggtgtggcga tacggggtga
      601 gacattaccg gtcgacagga tcgtgaactt gctcagcaaa acgaggaata tcgtctacta
      661 ttacaaaaaa gcctcttatt ttggaggatt ttttgttatt ttattgaatt tttaatttat
      721 tttaattgtg ttatgttttt tggggggggt tattggcggg tttcaaaacc ttattagccg
      781 tgaataggaa gagcataaag ttggctgcgg cctgctgaat aaagatgaaa acgaaccgtg
      841 ggcaaggagg aaccttcaat gaaaacctat ctcgaatatc aggatgaaaa gtctaataag
      901 ttctgggaga ttgaaataaa cggaactaac tttacagtac gctacggtaa gatcggggcc
      961 gacggacaga cactgaccaa gacattcggg tcggaagaag aggccaaaca ggaagcggac
     1021 aagctgatcg cggataaggt gaagaagggt tatagacaac cggacggtca agaggttact
     1081 tctcaagcaa tgaaaaattc tcaagcggag acatctgctc gggcacttcc ggatattgaa
     1141 acaataagcg caaaagttac acagatgctt gagaactggt ccggctttga gcttgaaaaa
     1201 gacttgccaa ggtatcgtcg gttattgcaa caacttgaat cgggtcaagg catcggcgaa
     1261 cttcatcaga ggtttagtga aaaactttta gacggggctg cagagaaaag acttgtcttg
     1321 gcttatgccc atcagagcaa aaaaattgat tactacgacc taagcccctg cggcagatgg
     1381 ttggctgtag ccagcagcaa tgatgacaat gatcaagggg caatccaggt ttgggagctg
     1441 gcttcgggtc gttgtgtcaa tgcattcggc acggtggaat acggttttgg atggcccggt
     1501 gaaaaggaga attgtctcaa gtggagtccg gacgggatga aactggcagc cagttattac
     1561 ggcacaaaca ttggggattt taaagcattt gaaaataata aaaaaggtaa taccgtcgag
     1621 ccggggactt gtattatcga tggatacggg atggaccggc cggctaattt ttgttgggaa
     1681 ccagccggcc aaagtctggc agtcgatgtg cggaccgccg gcggaggtat aaactgctgc
     1741 atcgtgcctt tgaatggcgg gcagattgat atttcaaaaa tgaatgaagt cttcaccggt
     1801 gatggccttg aagatattcc ccgttttgat gatcgccatt tttattggag caaggacggc
     1861 aagtttattt acggacttgc ggatggtaaa gtgatggtcc tggatgccgt caaccgttct
     1921 ttccggtatt gggatcatca ggctgatgat cttaatcagc catttagtcc ggaaggctgt
     1981 tattttatca ccaaacaagg caatcgggtt caacttcatt ctttagccga aatgatatcc
     2041 gaaatcacat tagattttga gccgaagttc agttattggc tgccgtccgt ccaagagccc
     2101 cggtttgttt tggcggatgc caatcatgtg gtgttgtatc ataattcggt agaaatgcaa
     2161 agatttgata ccaaatcaaa tatccaggcc attgaagttg caaaagacgg acagaacgcc
     2221 gtattgttat gcgacacctg tattgaagtt tggtcgctgg cccatactcc ggtttgtcga
     2281 cagatcattc aatccgacca tgtaatgaaa gatatccggc ttggggccga taacatgctg
     2341 gttgtttttt gcaacgaccg attggacgac cttaatgaag atgaagctaa tgttttagaa
     2401 ttttgggacc tgaacgccga tgaaaaaatc gccgaacacc ttatccgggg tctttatgaa
     2461 tcagaggatg agctggagga cctcatggaa gagttggaca atggagatca agaaatcggc
     2521 gcagtcatga tcaaaaggga taagcggggt tattggggag ctgtagttaa agcagaccgc
     2581 tttgtattgc cttcaaagaa attaaaaaaa gatctatctt tattgaatcc ttatttaagc
     2641 tttgttatag aaaaaaggtg gtcctggccc tggcattggg caaatgggac ccaacatatc
     2701 gaagttggtt cgaaaaaagt taaaaaggat gagtatgccg aagagcggga actgttgcgg
     2761 caaaaaatgg aagacatcag caatggtggt gaaattgatg ccgcaataga tgatactggt
     2821 tatacggctt taatggtggc ggctaaaatc ggtgatattg atctgatgaa acaattaatt
     2881 gagaccaagg cagatgttaa tgccaagacg aagtccggta caactgcttt aatgttggca
     2941 tgtaactgtg gccatacaga agctgtacgg ctgttgattg aagccggggc taatgttcat
     3001 cataaaaatg aatatgaaga aacggcttta gggtgggcgg tcgatccgga caaacctgag
     3061 ataataaagc tattgatcga tgccaaagcg gactttcaag caaaggatga tcaggggtat
     3121 acgccattaa ttgtagctgc cgctaaaggt tgtactgaaa ttgtaaaatt cttgcttgag
     3181 aaacaggtgg agatcgacgc cagaaattat aatggcgaca cagccttaat cctggcagcc
     3241 aagcaaggtt gcaccaaagc cgtcaaactt ttgattgaag ccaaagcgga cttggacgct
     3301 aagaataatg acggtaatac tgccttaatc ctagctgccg gctataaata ccttgaaatt
     3361 gaaaaacttt tgatccatgg cggatcaaac ccctatgaaa agaataacga gggtaagacc
     3421 gcgtataatg atgatctttt gattttaata ttattcgatg cggttactaa aaacagtccg
     3481 gaatcgataa aatcttatat taagaatatc ggtaagcttg atattaccga tgatcaaggt
     3541 ctgaccgcgt taatgcttgg ggcccaactg ggacagatgg aggcggttaa aactttaatc
     3601 gttgcaagag caaaaatgga cctgaccaat aaaaagggtg aaactgcttt gatgctggca
     3661 gaaaacaacg accatcatga tgttgccgaa ctattaaaaa gagaagcagc agtgggccaa
     3721 gcagttgaca gattttttga tgcaattaag gaaaacgatt tggagttgat aacctcgtat
     3781 ctccatagcg gctatgatgt gaaccggaaa aatcaagagg gatgtccggc attggtctgg
     3841 gcggcgcaaa atgggcaggc agccgtggtt aaactgctaa ttgaggcaaa ggccgaggtt
     3901 aatgcaacat tttataaatc gatatatggc gacagaatga ctgcattaat ggccgcgggt
     3961 gatgatgccg agataacaaa agtcctgctt gattcaggag ccgatgtcca tctgaaagat
     4021 gacaaaggaa agactgcttt aatatgggcg gtcataaaaa acaatcttga agtgatcaag
     4081 ttattgattg caggccatgc cgatgtcgat gcaacagata aatatggtga gtcgaccttc
     4141 ctaatcgccg catttaaggg aaatgttgaa atacttaagt ttttgtctac agccggtgct
     4201 gatatccacc taaaaaatag ccgtggtgag acggcattga tacaagcaac ggatcgtggc
     4261 aatatcgaag ccgtaaaaca cctgattgaa gccggcctgg acataaatgc gaaaaataaa
     4321 gatggagaat cagctttatt tctagctgcg aataataact atcccgccat attggagttg
     4381 ctgattaatg cgggagccga aatcagtgta tatgatggcg aaggtgagaa tgtattgata
     4441 aaggcggtac agaagagata tattgaagtg gtgaagattt taatcaaggc caaggttgac
     4501 gttaacgcta aagatcggtt tgaccgcacc gccttatcga tagccaatgg acaagaagac
     4561 agtgaaatga ttaaactgtt gcttgaggcc ggtgctgata ctacaaatgc ttgaacaatt
     4621 aggggatttc atcttgaaag aaaataaccg ttgcaacgag gtgagcaacg cttgtggagg
     4681 caatggtaaa acaggctgta tcttctcaga ttgaattcat tgtggatttg gatgaaaccg
     4741 ttatcggttc ccgttgtcgg gaatcgctga tccgtcaagc agttggtaat ggagaatgta
     4801 atgtggttct aaaagagatg acgattatgg gatttggcat tttggattac agtttctttg
     4861 agaatggttt catcaagtta ttgatcgttg ggagcggatg gcggcggaga ggctttggac
     4921 ggctgctggt ggagaccttt aaggaacaat gccggaccga aaaactcttt acttcgacca
     4981 atcgttccaa ccggcccatg cagatgcttc tggcgggttg tggctttgag cgatgcggtc
     5041 tcgtagaacg attggatgaa ggagatcctg agttaatcta ttgttttgaa cgaaaccgtc
     5101 ggactttggc atcaccgaaa tcgcaagcac ttaccgggtt tgatgataag ttttgggggg
     5161 ctgtcgatgt tcttgtggcc ggagcggaac ttgtgattga tcgtccgaag ggcagttgtc
     5221 acccgagatt tccggaggtg atatatccgg tggactacgg ttatttgaaa gggaccacgg
     5281 cgatggacgg cgggggaatt gatctttgga gcggaacagg gaaggaccgg agggtcgcgg
     5341 cgatcatttg caccgtggat ttaaacaaaa aggattcgga gatcaaattg cttttcggtt
     5401 gtaccgccgc cgaacaggaa ttgatttacc gttttcataa tgaatcagag tatatgaagg
     5461 gcatcctgat accgagactc tcgtgaaagg aagtgttcgt ttgttcaacg tagttgaagc
     5521 aagcatcgca gatttacagg ccgccatggg cgcgggtcag gttacttccc gagagttgat
     5581 tctgatgtat atggaacgta tcgccaagta cgacaaggac ggtccgaaac tcaatgcgat
     5641 tctggaaatc aatccggatg catt
//
LOCUS       DASVMB010000106         8119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_88364,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000106 DASVMB010000000
VERSION     DASVMB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88364"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VF531_03400"
     CDS             complement(<1..335)
                     /locus_tag="VF531_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7713044.1"
                     /translation="MSPIFHSDRVAFENSAPENDCRILLYGDSISKGVVYDEQKQKYV
                     VIAENYAALVQNRLKGIIHNAARFGNTIIKAVQRFPAELQETKPDVVVIEFGGNDCDY
                     NWEEIAANPG"
     gene            complement(400..1098)
                     /locus_tag="VF531_03405"
     CDS             complement(400..1098)
                     /locus_tag="VF531_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HEX7713045.1"
                     /translation="MTAVYDFHTHSFLSDGELGPTELIRRAVVNGYQAIAVTDHVSPG
                     NLRRVLAEIRRDCRMAETYWPITAIPGVELTHVPAGTIAELAKEAKESGAELVVVHGE
                     TLVEPVESGTNLAAVSCREVDILAHPGLISREAAELAVKNGIFIEISARNGHNVGNGH
                     VVGIGRQVGVRFLVNSDTHAPENLLTSVWAETVARGAGLSDVEAGVTLTENPFRLLER
                     VKARHEAIKKTTGE"
     gene            complement(1124..1303)
                     /locus_tag="VF531_03410"
     CDS             complement(1124..1303)
                     /locus_tag="VF531_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713046.1"
                     /translation="MSIITPFGAELSLKQLQTSLQYEISPRPSLVLWPPDPGGTEWLL
                     EVFDPLASKGQALKW"
     gene            complement(1306..1554)
                     /locus_tag="VF531_03415"
     CDS             complement(1306..1554)
                     /locus_tag="VF531_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HEX7713047.1"
                     /translation="MTKTSELREREVVNLLDGRRLGLASDLEIDAESGKIRAIVVPGP
                     GKFLWLFGKSEDFVIPWESIRKIGVDVILVETPNYVNG"
     gene            complement(1830..2123)
                     /locus_tag="VF531_03420"
     CDS             complement(1830..2123)
                     /locus_tag="VF531_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX7713048.1"
                     /translation="MKQLINWQVFLLAFSTLFIAEMGDKTQLAVFTLVTESKAPLTVF
                     LGASLALMVVTLIGALLGGVVTRFIPTFYLKIGASLLFIGIGVYTLWETLLSR"
     gene            complement(2385..2461)
                     /locus_tag="VF531_03425"
     tRNA            complement(2385..2461)
                     /locus_tag="VF531_03425"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2425..2427),aa:His,seq:gtg)
     gene            complement(2736..4184)
                     /locus_tag="VF531_03430"
     CDS             complement(2736..4184)
                     /locus_tag="VF531_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX7713049.1"
                     /translation="MNQSSLTESTDFELLDNPKQLQVSTLPPVWAAKAIFYQIFPERF
                     YNGDPGNDPPGKVPWNSQPSCDNFFGGDLKGIIAKIPYLKQLGVTALYVNPIFDSPSN
                     HKYNTRDYLRIAPEFGDAATFRSLIDQLHQAGIRIIIDGVFNHTGDTFWAFQDILKHG
                     EKSHFKEWYHCREFPIRQSPPNYECWWNFGTLPKLNHQNPEVVRYLLRAVAFWTSIGI
                     DGWRLDVPNEVTMDFWRVFRRLVKSINPEAFIVGEIWDEPFSWLSGDTCDAVMNYRWR
                     DAVIRYFAQGTISAEQFKMELLNNRSNLPWEYIVSAYNLLGSHDTPRFLTVCGEDRRK
                     FLSALAFQFTYPGIPALYYGDEIGLTGGKDPECRKPMCWDETGQDQMILRETRQLTKL
                     RMQYTSLQTGSYHDLHLGDGIFGFVRNGKSEQILACFNMNRDCRSIQLPPEWSNGWEP
                     IYAVGCGLSGLSYPELPPCGVRIFKRSRTLSR"
     gene            4478..6085
                     /locus_tag="VF531_03435"
     CDS             4478..6085
                     /locus_tag="VF531_03435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7713050.1"
                     /translation="MAFSHSSSFTSGCRPKPGRNPRFEKLVLGYRIMMISVNYLMLFY
                     WGQPQRDGAYVPFQAMLILLGYILIAWCGPYFINVFRVNQILSLADILLVSLGIYYLP
                     GNLFAIATVYLVGIIINAAFYGQFLSSAALAVISCLGFTLGFQGGIPSSANQWFFIVN
                     LGLTTLLMVFGSLQIERDLRTAEREKSESKKRLHHLEGLSRITKEIAGELELDKLLPL
                     IIRKANQLTRTKVGGIILQDNDGIYRIKAINGLSSSFLERELTSGVGFLGKALLEKRI
                     CFDQASNDTGPMDLEHYHYAVAAPIHSKDDSMGLVFLLSDTADGTFSKNDRLILQTLG
                     EQAAIAVINARRYKMTASLSLNDYLTGLGNIRFFYRELEHTLAVATRYRQSCSLMIVD
                     SDSLKQINDCYGNAQGFRHIKELAGILKNTIRSSDMIARYDRDMFMIILPQTSLTEAI
                     QLGTRIQHQVALSPLIIDGQTVNTSVCIGVATFPADADNVQNLVSLVEILLHQAKQGG
                     KNHLKSTLSTSENNNNNNTCEVHTLQI"
     gene            6133..6732
                     /locus_tag="VF531_03440"
     CDS             6133..6732
                     /locus_tag="VF531_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7713051.1"
                     /translation="MLYQIFKNFFFILFKVFHRMKVIGAEHVPMTGPVILAANHVSNW
                     DPLVVGSAAPRQLVFLAKKSLFKIPLVGLLMRGWGATPVERGHSQRSTINFWLNVLKQ
                     GKAIGIFPEGKRNLEHTGQMLKLQSGLAMLALNTGAAVVPVAVLNSDRIFRGFKQLKV
                     IIGTPITFQDQPELDRKELYDRINTRITAEIMELKKKYT"
     gene            complement(6799..7326)
                     /gene="raiA"
                     /locus_tag="VF531_03445"
     CDS             complement(6799..7326)
                     /gene="raiA"
                     /locus_tag="VF531_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7713052.1"
                     /translation="MLVTVTGKNVAVTEALREYAEKKVTKIGKFFEKSPLEAKVTLST
                     ERGKHVVDITVQVNGILLRGEEKTTDMYISIDGAIEKIERQVEKFKTKINRKFHEENY
                     VTLSSSSPAEETPQPVIKRTKRFAIKPMTAEEAVMQMDLLGHDFFVFSNSETEEVNVV
                     YRRKDGNYGLIEPEF"
     gene            complement(7591..7791)
                     /locus_tag="VF531_03450"
     CDS             complement(7591..7791)
                     /locus_tag="VF531_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HEX7713053.1"
                     /translation="MERIKCSVASCKYNEGADTCQADQIKVRDNFGTTDTMEIGSLEG
                     DTKARTSMETCCETFAPKKSNK"
     gene            complement(7926..>8119)
                     /locus_tag="VF531_03455"
     CDS             complement(7926..>8119)
                     /locus_tag="VF531_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713054.1"
                     /translation="FQRCWRVQDCNWVNCPAYQSQEEKCWLVPDTLCQDGISNGSVTD
                     KRPMCHQCEVFKINTKLDV"
BASE COUNT         2168 a         2031 c         1758 g         2162 t
ORIGIN      
        1 cccggatttg cggcaatctc ttcccaattg tagtcgcaat cgttaccgcc gaattcgatg
       61 acgacgacat ccggtttggt ttcttggagt tcggcgggga aacgctggac cgccttgatg
      121 atggtatttc caaaacgggc cgcattatga atgatccctt taagccggtt ttgaactaga
      181 gcggcataat tctcggcaat aaccacatat ttctgctttt gttcatcata gaccacaccc
      241 ttggagatcg aatcaccgta taataaaata cggcaatcat tttccggggc tgaattttca
      301 aaggcgaccc gatcggaatg aaagatagga ctcatgttaa caccagcttg tttagttttg
      361 aagttcattt agtaaaacga agtccatcgc ctaaatcttt cattccccgg ttgttttttt
      421 tattgcctca tgacgagcct tcacccgttc caacaaacgg aatggatttt cggttagcgt
      481 cacacccgcc tcgacatccg ataaaccggc ccccctggca accgtttcag cccaaacgga
      541 agtcaataga ttttcggggg cgtgtgtgtc ggagttgact aaaaatcgca ctccaacttg
      601 tcttccaatg ccgaccacat ggccattgcc gacattatga ccgttccggg cggagatctc
      661 gataaatatg ccgtttttaa cagccaattc tgccgcttca cggctgatta acccgggatg
      721 agccaaaata tcgacttcgc ggcaggaaac agccgccaaa ttggttccgg attctaccgg
      781 ttccaccaag gtctcaccgt ggaccactac caattcggcc ccggattcct tggcttcctt
      841 ggctagttca gcaatagtgc ccgccggcac atgggtcagt tccaccccgg gaatcgcggt
      901 gatcggccaa tacgtttcgg ccattcggca gtcgcggcgg atttcagcca agactctacg
      961 cagattccct ggactgacat gatcggtcac ggctattgcc tgataaccgt tgacaacagc
     1021 cctccggatc aactctgtcg gccccaactc tccgtcgctc aagaacgaat gggtgtgaaa
     1081 gtcatatacc gccgtcatca cttcatcctc cttacttaag aatctaccat tttaaggctt
     1141 gtcccttgga ggccaatgga tcaaagactt caagcagcca ttcggttcct cccggatctg
     1201 gaggccatag taccagactg ggacggggac tgatctcgta ttgcagcgag gtttgaagtt
     1261 gttttaagga tagttccgct ccgaatggcg taataatgct caaactcacc cgtttacata
     1321 gttgggagtc tccaccagaa tcacatccac accaatcttc cggattgatt cccaggggat
     1381 gacgaaatct tcactctttc caaacagcca gagaaattta ccgggacccg gcaccacgat
     1441 cgcccggatt tttccacttt cggcgtcaat ctccaaatca cttgccaaac ccagtcgcct
     1501 gccgtctagc aggttgacga cctcacgttc ccgtaattcc gaggtcttcg tcaacaaaca
     1561 aactcgcccc cttaacccta actccggcaa ttcaaggctt ggatcccttt ccaggccgtc
     1621 agttttcaat attccgggca tgcgcgcggg catgaaacgc ggatggtggt gatgaaagcc
     1681 aacccatctc ttctataggg ttcatcagag atcatattcg gcgcaggggg cttttggaac
     1741 atatggtaga ggttcgggaa gaaatcgtac cggtcccctg tggttaggaa tctgattacg
     1801 aaaatgactt gattatcgcg atagcatggc taccttgata acaaggtttc ccagagggta
     1861 taaacaccga ttccgatgaa aagaagactg gctccgattt ttaagtaaaa ggtggggata
     1921 aaccgggtaa cgactccacc caagagcgct ccaatcaacg tgaccaccat caacgccaga
     1981 ctcgcaccta aaaaaaccgt caacggcgcc ttggactccg ttaccagagt aaacaccgcc
     2041 agctgggttt tatcacccat ttccgcaatg aacaaggtgg agaaagccag taggaaaact
     2101 tgccagttaa ttaactgttt caaagttacc cctcccaatt tccttaacga ggctgagctc
     2161 aaaatacttg ccgcacattg gaacgttgat atgcattcat ccgtttaacc gatacgccat
     2221 ctttcatgtg acttatctta atagaagaaa ctcatccgga ttggtgattc attggattaa
     2281 tatgacggtg gtcctatctt ttttattcat aatccctgct ttatttttac catgcgaata
     2341 aatgagtaaa aaaaaaggga ctactgtccc taatcttcaa atcttggggt gaacgatggg
     2401 attcgaaccc atgacctcca gggccacaac ctggcgctct aaccagctga gctacgccca
     2461 ccatgtgtgc aacagatata ttatcataga atgaaaggac tgtcaatgat ttatttgatg
     2521 agcatatcag gaaacaatac cagcctattc atttgaaggg acagacacgc gactcaattc
     2581 agaaaagtcc tccaccaaag agtatatgtc aagaaatact ccctagaaca tggaataaat
     2641 catagaggcc gcctggtttg aaccaaacgg ccctctatcg agagaaatca aaatgaatcc
     2701 gcagacctgc ggcatcccga atatttcctc aaaccctaac gggacaaggt tcgacttcgc
     2761 ttgaagattc gaacaccaca aggtggcagt tccggatagc tgagtcccga aagcccgcaa
     2821 cccacggcgt agatcggctc ccagccgttg ctccattccg gaggcaactg aatactgcgg
     2881 caatcacgat tcatattgaa acaagcaaga atctgttcgc tttttccatt ccttacaaac
     2941 ccgaagatcc cgtctcccag atgcaaatcg tgatacgatc cggtctgcaa tgaagtgtac
     3001 tgcatacgga gtttcgtcag ttggcgtgtc tccctcaaaa tcatctggtc ttgacccgtc
     3061 tcatcccaac acatcggttt ccggcactcc ggatctttcc caccggtcag gccgatctca
     3121 tctccgtaat acaatgccgg aattccgggg tacgtaaatt gaaaagccaa agccgacaag
     3181 aactttcttc gatcctcccc gcatacggtg agaaagcgtg gagtatcatg acttcctaac
     3241 agattgtaag cgctgacaat atattcccat ggtaaattgg accgattatt cagcaattcc
     3301 atcttaaatt gttccgctga gatcgtacct tgtgcgaaat aacgaattac cgcatcgcgc
     3361 caacgatagt tcattactgc atcgcaagta tccccgctta accaggagaa tggttcatcc
     3421 caaatctccc ccacaataaa ggcctcaggg ttgatcgatt tcaccaaccg ccggaaaacc
     3481 cgccagaaat ccatggtgac ctcattgggt acatccagcc gccatccgtc gataccgata
     3541 ctggtccaga aagctaccgc cctcaggagg tagcggacaa cctccggatt ttggtgattc
     3601 aatttgggca gagtcccaaa attccaccag cattcgtagt tgggcggaga ttgccgaatt
     3661 ggaaactcac ggcagtgata ccactccttg aaatgggatt tctccccatg cttgaggatg
     3721 tcctggaagg cccagaatgt gtctccggtg tgattgaata caccatcgat gataatccgg
     3781 attccggctt gatgtaactg atcgataaga cttcggaatg tggctgcatc cccgaactcg
     3841 ggagcgattc gcaaataatc acgtgtattg tatttgtgat tggaaggtga gtcaaagatt
     3901 ggattaacgt aaagggctgt tacacccaac tgttttaaat acggaatctt ggcaataatt
     3961 cccttcagat cgccgccgaa aaaattgtcg catgatggct gtgagttcca tggaacctta
     4021 cccggcgggt cattcccggg atcaccgtta tagaaacgtt cgggaaatat ttgatagaaa
     4081 atcgcttttg ctgcccaaac aggcggtaac gtagaaacct gaagttgttt gggattatcc
     4141 aacaattcaa aatcagtcga ttctgtcagg cttgattggt tcattatatc accccttgca
     4201 gaactttcga tgggtctatc ccaaagaata atactaatgt tacaaaaccg tttctgcaat
     4261 gggtaattga aggagatgtt accgattaaa gccaagaaat tttacaaatt aaaagggaat
     4321 tactagcaaa tgcagaatga ttaaaatcgg ttaactctgg ttggtcatac gtccattggg
     4381 aattgacgat attttcgtga accatttcat taacagggga ataatactat tggccaccgg
     4441 agggagaata acaaagttag aaaggttgtg ttgcggggtg gcgttctctc attcgtcatc
     4501 ctttacgagc ggttgccgcc caaaaccggg ccgaaatccc cgatttgaaa agctggtttt
     4561 aggttatcgc atcatgatga tctccgtcaa ctatttgatg cttttttatt ggggacaacc
     4621 gcagcgcgat ggtgcttatg tgcctttcca agcgatgctg atccttctcg gctacattct
     4681 tatcgcatgg tgcggaccct attttatcaa tgtttttcgg gttaaccaaa ttctttcact
     4741 cgccgatatt cttttagtct cactagggat ttactacttg cccggtaacc tattcgctat
     4801 agctactgtc tatttagtcg gtatcatcat taacgccgct ttctacgggc agttcctttc
     4861 cagcgcagcg ttggccgtta tctcatgtct gggatttacc ctgggttttc agggcggcat
     4921 cccgtcatcg gccaatcaat ggtttttcat cgtcaatctg ggcttaacga cattactgat
     4981 ggttttcgga agcttgcaga ttgaacgtga tcttcgcaca gcggaacgtg aaaagagcga
     5041 atccaaaaaa agattgcacc acttagaggg tttatcaaga atcacaaagg agatcgcggg
     5101 agaactcgaa cttgataaat tactcccctt gattattcga aaagccaacc aattgactcg
     5161 cacgaaggtt ggagggatta tcctacagga taatgatgga atctatcgga ttaaagccat
     5221 taatggccta tcttcttctt ttttagagcg agagctcact tccggggtag gttttctagg
     5281 aaaagctctc cttgaaaaga ggatttgctt tgatcaagca tcgaatgata ccggaccaat
     5341 ggatctggag cattatcatt acgcagtcgc tgcaccgatt cattccaagg acgattcaat
     5401 ggggctcgtt ttccttctca gtgataccgc agacggtact ttctccaaga acgatcgctt
     5461 gattctgcaa accttgggcg agcaggccgc aattgcagta atcaatgccc gtcggtataa
     5521 aatgactgcc agtctcagtc ttaacgacta tttgactggt ttgggcaata ttcgcttttt
     5581 ctatcgggaa cttgaacata ccttggcggt agccacccgt taccggcagt cttgcagttt
     5641 gatgattgta gattcggatt cattaaaaca gattaatgat tgttacggca atgctcaagg
     5701 atttcgacac attaaagagt tggccggaat ccttaaaaac actatccgca gctccgatat
     5761 gatcgcccgt tatgaccggg atatgttcat gattatcctg cctcaaacca gtctgaccga
     5821 ggcgattcaa ctgggtaccc ggattcagca tcaagttgct ttatctccct taatcattga
     5881 cggccaaacc gtgaatactt cggtctgtat cggggtggcc acctttccgg cagatgccga
     5941 caatgttcaa aacctggttt cccttgttga aattttacta catcaagcca aacaaggagg
     6001 aaaaaaccat ttaaaaagca ctctatctac atctgaaaat aataataata ataatacttg
     6061 cgaagtacat acgctccaaa tctgaagaac tccgcccatc gatcatgaat aaccacaaaa
     6121 aggagactca acgtgcttta ccaaatcttc aagaatttct ttttcattct gtttaaggta
     6181 tttcaccgca tgaaggttat aggcgcggaa catgttccta tgaccggtcc ggtaattttg
     6241 gccgccaacc acgtcagtaa ttgggatccg ctcgtcgttg gctccgccgc tccccggcaa
     6301 ctggtctttc tggcaaagaa gagcctcttc aaaatcccgc tcgttggtct actgatgcgc
     6361 ggctggggag ctactccggt ggagcggggt catagccaac gttctacaat caatttttgg
     6421 ctgaatgtat tgaagcaagg aaaggcaatc gggattttcc ccgagggaaa acgaaacctg
     6481 gaacataccg gccaaatgct caagctgcaa tccggtctgg cgatgctggc tttaaatacc
     6541 ggcgctgccg tcgtcccagt ggccgtactc aactcggaca ggatctttag aggatttaaa
     6601 caactgaaag tgattatcgg cacccccatc acctttcagg atcaacccga acttgatcgc
     6661 aaggagctct atgatcggat taatacccga atcactgcgg aaatcatgga actaaagaaa
     6721 aaatatacct aagtgaaaga agcggcaatc gccgcttctt ttatcgtgaa taccatattc
     6781 aatgaaagta actcagtgct aaaactccgg ttcgattagg ccgtaattgc cgtctttacg
     6841 tcgataaacc acattgactt cctcggtttc actgttggaa aaaacaaaaa aatcatgacc
     6901 gagcaagtcc atctgcatga ctgcctcttc agcggtcatc ggtttaatcg caaaccgttt
     6961 ggtccgcttg atcaccggtt gaggggtctc ctccgcggga gacgatgaac ttaaagtgac
     7021 ataattttct tcatgaaatt tacggttaat cttcgtttta aacttttcca cttgacgctc
     7081 gatcttttca atcgccccgt cgatggaaat gtacatatcc gtagtctttt cttcgccccg
     7141 cagtaagatg ccgtttacct ggacagtaat atcaacgacg tgttttcccc gctcggtact
     7201 tagggtcact ttggcttcaa gcgggctttt ttcaaagaac tttccgattt tggtcacttt
     7261 tttttcggcg tattcccgta atgcctcggt aactgctaca tttttcccgg tgactgtcac
     7321 caacatcaag catccctcct agtttaacat tctcccggac cctccgccca tggcggaatc
     7381 gctttaaatc aacaaatgct tgtgcgcgct tccactttcc gggaatcaca gatgtaacca
     7441 ggtgcctaca tggaccttac ataatatatt acctgttcag cttaaaaatc cttcccggat
     7501 cgcatataaa aaaataaaaa aacttgtgct ataacgaatc cacggctggg atgctgggat
     7561 tgaaaagcac aagccaaata atgacgtcgt ttatttgttg gattttttgg gagcaaaggt
     7621 ttcacagcaa gtttccatcg aggtcctggc ttttgtatca ccttccagtg aaccaatttc
     7681 catggtatcg gtggtcccaa aattatcccg taccttgatc tggtccgcct ggcaggtatc
     7741 agccccttcg ttatacttac aacttgccac gctgcatttg attcgttcca taaaatcacc
     7801 tccactttgg gtattatcta ccaatcatgg aggcgttatt cctgtctgca gaataattca
     7861 ttgaatcatg gccggggcga taaggtacca aggttctatg aaatcagatt aaagcgagta
     7921 cgatattaaa cgtccaactt ggtattgatc ttaaatactt cacactgatg acacatgggc
     7981 cgtttatcgg ttacagaccc attggagata ccatcctgac acagagtatc cggcactaac
     8041 cagcattttt cctcttgtga ctggtaagcc gggcaattaa cccagttgca atcctgaacc
     8101 cgccagcaac gttggaaat
//
LOCUS       DASVMB010000107         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_601675,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000107 DASVMB010000000
VERSION     DASVMB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601675"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VF531_03460"
     CDS             <1..936
                     /locus_tag="VF531_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7713055.1"
                     /translation="ACLVIIDSCFLITYFATKWGNNLVKSNAEKEEQTRGLLDQLEKT
                     YQQALQSSNQVSRVASQIAMGNQELSQRTQEQASTLEEYAATIVEVSSSVQEVAIHSA
                     EAGRISQATLTAVMEGNHSLSETLKAIREISSDSAQISEIIKVMNDIAFQTNLLALNA
                     AVEAAHAGEEGRGFAVVASEVRNLAVRAAESAKEIERLIAGIIGHIQKGNQLAQDSAK
                     ALNQIVENTKKTSEVISLVSMAMQEESSASQQIQTAIEQLNQATQENAALVEEISASS
                     QSLRVEAENLNHLVDQGTQKISLDKKIIGSKDQIK"
     gene            complement(1044..1721)
                     /locus_tag="VF531_03465"
     CDS             complement(1044..1721)
                     /locus_tag="VF531_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7713056.1"
                     /translation="MNIAESWLPPGKDCGLCGNGSCREFLHSVQNQEKYYTECPFYQK
                     ETDDKTHGQAGEQMRQAKYTGKDILGHAYDFILSALPGEISPRKIVLPFRPDLVDILD
                     IHKGDYIMGRPMGAGCPVPHVQYVIDADRITGVLTTWVVGPKISREHSVKDVKAYHMI
                     GFEGLAEEIRNEPTVGYRMTFLPGCCMMNLNHTALVNMVLETSYGKHIHVEDIRILAG
                     IDESKNR"
     gene            complement(1724..2434)
                     /locus_tag="VF531_03470"
     CDS             complement(1724..2434)
                     /locus_tag="VF531_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7713057.1"
                     /translation="MKLIIVAGPPSAGKTALIKQVVRKLINEMRIAFLKIDVVKAFED
                     QELHEEFHIQTKKIYSGDLCPDHAGVMVLGDAIQWAKDNDTDLFIVESAGLCLRCSPY
                     LNQGLGIIVLSSISGIHAPEKMGSMVSLADIAVVTKIDLVSQAEREVMIQKVKEVHPN
                     IILLETNALQGTSMQRLYDLIRDSEEIDPDNLILKGNPPLGTCTICVGKKDIGWKHHF
                     GVVKKLDGEIADYLYRGE"
     gene            2837..3919
                     /locus_tag="VF531_03475"
     CDS             2837..3919
                     /locus_tag="VF531_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX7713058.1"
                     /translation="MAVFCIPGNFPTINEAVSSVAPGDTILVGNGVYSEAVAIPEAKT
                     GIRIIADGEQVILDGNSLLEIAFNIMANNVEIKGFTIINYLTSGIRVSGPTGLIFLIG
                     SKLIQNIIMNITRGDGITLVDSFATLIWQNRIRGVGDHAVNITGSTMNTWTIENELNG
                     NGANGVINQTNANVGGTITRNRILANSGSGVQDNAGSNLIFANLITGNKLDGVDEASP
                     TAAAGFGGIVANCITGNRRDGVRLDQDRTVVTGNRVLNNLGTGVLIDTNLNVVQQNVI
                     TANMNNGITLTPDAQGNLAVRNIITRNTPFDIDRENPQNDLVENCFQTSNPSGLYTSV
                     PAPPVCSPPQNTEETFSKDATDGRDG"
     gene            3919..4872
                     /locus_tag="VF531_03480"
     CDS             3919..4872
                     /locus_tag="VF531_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7713059.1"
                     /translation="MAVFRVPQDFGSIVQAVAAVQSGDTILVCPGVYHQTVIIPNDKP
                     GIRIIADSDRVILDGLSRMAAAFLIQGNNTEIKGFTIQNYLVAGIRASAPSGVIFLSG
                     TKLIHNRIINISPGAGIEVLGTFATLIWQNQVSGCGANGINLQARDTWVVANQIDTNH
                     GNGIAALTQTTVGNMMIDNRVTDNGMNGIADSAGFNLIYDNLISGNGIHGVHELSGQG
                     PAGLVGNVISNNHESGIQLDTMGHMVTGNTISGNLSGIRIEGDFNTIESNRIFGNRVS
                     GITIGATASNNLVIRNQFNQNLPIERINPRNDLIENCGVPC"
     gene            4927..5115
                     /locus_tag="VF531_03485"
     CDS             4927..5115
                     /locus_tag="VF531_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713060.1"
                     /translation="MEQGREAVFPWIDEQKKIIASLEGPEQNEPQPQVLEILKQDLER
                     ISGETGSFSLEALKPPVQ"
BASE COUNT         1408 a         1294 c         1212 g         1263 t
ORIGIN      
        1 gcgtgtctgg tgatcatcga cagttgtttt ctgatcacat atttcgccac caaatggggg
       61 aataatcttg tcaaatccaa tgccgaaaaa gaggaacaaa cccgcggact gttggaccaa
      121 ttagagaaga cttatcaaca agcgcttcaa tcatccaatc aagtttcgag ggtggcctct
      181 cagattgcca tgggtaatca agaactatcc caacggaccc aggaacaggc ttcaaccttg
      241 gaagagtatg cggcaacaat tgtggaagtc agcagttcgg tccaggaagt cgccatccac
      301 tccgccgagg cggggcggat atcccaggca acattaaccg ctgtcatgga agggaatcat
      361 tccctctccg aaacccttaa ggcaattcgg gaaatctcca gtgacagcgc tcagatcagt
      421 gagatcatca aagtgatgaa cgatatcgcc tttcaaacca atctattggc attaaatgcg
      481 gcggtcgaag cggctcatgc cggagaagaa ggacgggggt ttgcggttgt cgcttcggaa
      541 gtacgcaatt tggcggtccg ggcagcggaa tccgctaagg agatcgaacg cttaatcgcc
      601 ggcattatcg gtcatattca aaaaggcaac cagttggccc aggattcggc caaggctctc
      661 aatcaaattg tcgagaatac caaaaagacc tctgaggtca tatccctggt atcaatggcg
      721 atgcaggagg aatcctcagc ctcgcaacag attcaaaccg ctatcgaaca attgaatcag
      781 gcgactcagg agaatgcggc cctggtagag gagattagcg cttcaagtca gtccttgaga
      841 gtagaagcgg aaaatttgaa tcacctggtg gaccaaggaa cccaaaaaat cagcttggat
      901 aaaaagataa tcggatcaaa ggaccagata aaatgattgt ttagatcgat cattgaaaga
      961 agattaaaaa atcatcaatt tttcttcgaa aaaagaagcc ttaccgtaca aggcttcttt
     1021 tttgatttat cccgtttgat acgctatcta ttttttgatt catcaattcc ggccaaaatc
     1081 cggatatcct ctacgtgaat atgcttcccg taactggtct ctaaaaccat attaaccagc
     1141 gcagtgtgat tcagattcat catacaacaa ccgggtaaaa aagtcatccg gtacccaacc
     1201 gttggctcat tccgaatctc ctccgccaaa ccttcaaagc caatcatatg ataagccttc
     1261 acatccttta ccgaatgctc ccgggagatt ttgggaccaa caacccaggt agtaagcact
     1321 ccggtgatcc ggtcggcatc aatcacatat tgcacatggg gcaccgggca acccgccccc
     1381 attggccggc ccatgatata atcgccttta tgaatatcaa ggatgtcgac taaatccgga
     1441 cgaaagggca acacaatttt ccgtggtgag atttcccccg gtaaagcgct aagaataaaa
     1501 tcatatgcat ggccaagtat atccttgccg gtgtatttcg cttgtctcat ctgctcgccg
     1561 gcctggccgt gagtcttgtc atccgtttcc ttttgataaa aaggacactc ggtgtaatac
     1621 ttctcctgat tctgaactga atgcaaaaac tcccggcagc tgccattccc gcataaaccg
     1681 cagtccttcc cgggagggag ccatgattcc gcgatattca tactcattca cctcgatata
     1741 gatagtcagc gatctctccg tcaagctttt tgaccacccc aaaatgatgt ttccaaccaa
     1801 tatccttttt gccgacacaa atggtacaag ttcccaaagg cggattgcct tttagaatta
     1861 aattatcagg gtcgatctct tcggaatccc gaatcaaatc atacaagcgc tgcatagaag
     1921 taccctgcaa ggcattggtc tctaaaagaa tgatattggg atggacctct ttgacctttt
     1981 gaatcatgac ttcacgttct gcctgactta ccaggtcaat tttggtgacg accgcgatat
     2041 cagccaagct aaccatggaa cccatctttt ccggagcgtg tatccccgag atggaactta
     2101 acacgataat ccccagccct tggttaagat atggagaaca ccgcagacag agacctgcgc
     2161 tttcaacaat gaaaagatcg gtatcattgt ccttggccca ttgaattgca tcccccaata
     2221 ccataactcc tgcgtggtcg ggacagagat ccccggagta gatctttttg gtttggatgt
     2281 gaaattcttc atgtaattcc tgatcttcaa aagccttcac tacatcgatc tttagaaaag
     2341 caattcgcat ttcattgatg agtttgcgga ccacctgttt gatgagggcg gtcttacctg
     2401 ccgaaggcgg tccggcgacg atgattaatt tcattccaag tctacctcca ttaatttaat
     2461 tattcccgga caatctgcct gtttgaaagc taaatctttc gattaatttg ggtttaccca
     2521 gtgcacccgg gtcatggttt tatcaccttc tttcatggcc ttatggattt cccgagccag
     2581 tatcttcata tagccggaag gatgaaggtt tcaaagattg acccctacta aaatatgcaa
     2641 aaagcactgg gatgtttact aaacccatgg gattgattat atttttcgtc ccggctacga
     2701 cgggcgattc aatctgggag aaatatactg ttgatacctt tgcatgattt tggcaagcaa
     2761 cttttctcac tttttccctg taccatgaac aataaaaccg ccggcggatt ataatttggt
     2821 gaaaggagga atcccgatgg cggttttttg tatcccgggc aacttcccca ccatcaatga
     2881 ggcggtgagt tcagtcgccc cgggagatac cattctggtt gggaatggcg tatattcgga
     2941 agcggttgca atacctgaag ccaaaaccgg gatccggatc attgccgatg gcgaacaggt
     3001 aatcctcgac ggcaacagcc tgcttgagat cgcctttaac atcatggcga ataatgtcga
     3061 gattaaaggt tttaccatca tcaactatct gacctccgga atccgggtct ccggtcctac
     3121 cgggttgata tttctgattg gttccaagtt gatccaaaat atcatcatga atattacccg
     3181 gggtgacggc attaccttgg ttgattcatt tgccaccttg atttggcaga accggattcg
     3241 cggtgtcggg gatcacgcgg tcaatatcac cggatcgacc atgaacacat ggaccatcga
     3301 gaatgagcta aatgggaacg gcgcaaatgg cgtaatcaat caaaccaacg ccaacgtcgg
     3361 cgggacgatt acgcgtaacc ggatcctggc gaactccgga tccggagttc aagataatgc
     3421 cgggtccaat ctgatctttg ccaacctgat tactggcaat aaattggacg gggttgatga
     3481 ggcttcacca acggcggccg ccggatttgg aggcattgtc gccaactgca ttaccggcaa
     3541 tcgccgggat ggggtacgat tggatcagga ccgtaccgtg gtaaccggta accgcgtgct
     3601 caataatcta ggaaccggtg tactcattga caccaatttg aacgtggtcc agcaaaacgt
     3661 gattaccgcc aacatgaata acggcatcac ccttactccc gatgctcagg ggaatctggc
     3721 ggttcgcaac ataattacac ggaatacgcc gtttgatatc gatcgtgaaa acccgcaaaa
     3781 cgatctggtg gaaaactgct tccaaaccag taacccaagt ggactataca cttccgtccc
     3841 ggctcccccg gtctgttccc ctcctcaaaa tacggaggag acgttttcca aagacgcaac
     3901 ggacgggagg gatggataat ggcggttttt cgagttcccc aagattttgg ctcgatcgtt
     3961 caggcggttg ccgcggtcca gtccggtgac accatattag tctgtccggg ggtttatcat
     4021 caaacggtca tcatcccgaa cgataagccc ggtattcgca ttatcgccga tagtgacagg
     4081 gtgatcctgg atggtttgtc cagaatggcg gcagcctttt taatccaggg caataatact
     4141 gagatcaaag ggtttacgat tcagaactac ctggttgccg gaatccgggc ctccgctcct
     4201 tccggtgtga tctttctctc cggaaccaaa ctaatccata accggattat caatatctcc
     4261 ccgggggcgg gaattgaagt attgggtacc ttcgccacct tgatctggca aaatcaagtc
     4321 tccggctgcg gcgctaacgg cattaatctt caggcccggg atacctgggt cgtcgcgaac
     4381 caaatcgata ccaaccatgg caatgggatc gcggctctga cccagaccac cgtaggtaac
     4441 atgatgatcg acaaccgggt gacggataac ggcatgaacg gcatcgccga tagcgccgga
     4501 ttcaacctga tttacgataa ccttatctcc ggaaacggaa ttcacggcgt tcatgagctt
     4561 tccggccaag ggcctgccgg attggttgga aatgtcatca gcaacaacca tgagagcgga
     4621 attcagctag ataccatggg acatatggtc accggcaata ccatctccgg taacctttcc
     4681 gggatccgca tcgaagggga tttcaacacc atcgagtcca accgcatctt tggcaaccgg
     4741 gtcagcggca tcaccatcgg agctacggcc agcaataatt tggtgatccg gaatcaattc
     4801 aatcaaaacc tgccgattga acgcatcaat ccccggaacg acctaatcga aaattgcggt
     4861 gtaccttgct agcagcctga ttttttgagg aaagcgctga aaattgataa gtaaggggga
     4921 ttgaatatgg aacaaggccg ggaagcggtc tttccgtgga tcgatgaaca aaaaaaaatc
     4981 atcgccagtc tggagggacc cgaacaaaac gagccccaac cacaagtgtt ggaaatctta
     5041 aagcaggacc tggagagaat ttccggggaa acgggatctt tctccctgga ggcccttaaa
     5101 cccccggttc aataggattt tcgatagatt cagactaaga catcagttat ttacgactct
     5161 cagccactga gagtcat
//
LOCUS       DASVMB010000108         4446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_381206,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000108 DASVMB010000000
VERSION     DASVMB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381206"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1156
                     /gene="dpsA"
                     /locus_tag="VF531_03490"
     CDS             239..1156
                     /gene="dpsA"
                     /locus_tag="VF531_03490"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HEX7713061.1"
                     /translation="MERISDLQAAVIGGDTRQVAVAEALANEFKTVKLYGHIAAETPT
                     AVLNTASLLETLSEVRVVVLPISGMNVVGMVRGRKIGEEIDFGSCLNALSPGTLIVTG
                     SLTSDWLQRCSELRLQIVQYAEDDEIAILNSIPTAEGALQIALEEMPITIHDSTVLVL
                     GFGRVGMTVARTFKALGARVKVAARRNDVLARAWELGCDRIPLSTASTLGMETLNESI
                     ANIDLILNTIPALIMDSTLLAKISSRTLIIDLASPPGGTDFEAAKQFHIRAILAPGLP
                     GKVAPQTAGAILVETIPRLIRNSLYGGDR"
     gene            1153..1746
                     /locus_tag="VF531_03495"
     CDS             1153..1746
                     /locus_tag="VF531_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HEX7713062.1"
                     /translation="MMLSGVKIGFAITGSHCTLGEVLPAMQQLKEEGAELFPILSPSV
                     RDTDTRFGKAADLYQKVVEISGHPPITTIAEAEPIGPRKMLDLVLVAPCTGNTMAKLA
                     QGISDTSVTMACKAQLRNNRPVVLAIATNDGLGTNARNLATLLNKRHIYFVPFGQDAP
                     HTKETSLNARMSLIPDTIASALEQRQIQPLLLVFHEA"
     gene            2081..3319
                     /gene="dapG"
                     /locus_tag="VF531_03500"
     CDS             2081..3319
                     /gene="dapG"
                     /locus_tag="VF531_03500"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7713063.1"
                     /translation="MRIIVQKFGGTSVATPEGRENVVKKVSEALKQSYRVVVVVSAMG
                     RAGEPYATDTLIGLIRSIKAQESPRDLDLLMSCGENISTVVMVQTLKAHGIEAQALTG
                     GQAGILTDGQFGNARILEIKPDKIMKCFDEGRVAVVAGFQGTTVDGAVTTLGRGGSDT
                     TAAALGVALKAEVVEIFTDVDGVMTADPRLVPEAKPLATMTYNEVCDMAHLGAKVVHP
                     RAVEIAMEGRIPLRIRSTFSENLGTLICDGSPIGNVEIRSDKVVTGIAHIQGMALVKL
                     KSKEDLNKTGQVLEIFQLMAKAGISVDMIQVAPANVSFIIKEDQIGQAKEVLDQLSLE
                     TSMEKGFAKVAIVGSGMRGVPGVMARMVHGLQQAGISIYHSTDSHVSIACLVRQDEMI
                     KALRALHEEFELGSKEETNG"
     gene            3312..4193
                     /gene="dapA"
                     /locus_tag="VF531_03505"
     CDS             3312..4193
                     /gene="dapA"
                     /locus_tag="VF531_03505"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7713064.1"
                     /translation="MVKFGKVLTAMITPFDPNLQVDYRAAQELAVRLIQSGSDGVVVA
                     GTTGESPTLTKTEKLTLFKAVVEAVGGEGIVIAGTGSYNTADSIELTKEAERTGVDGV
                     MLVAPYYNKPSQEGLYQHFAAIARETALPVIVYNIPGRTGVNITADTMVRLTEISNIN
                     AVKEASGNLNQVSEIYEKAPRDFQIYSGDDALTLPVMSLGGVGVISVASHIVGRKLQE
                     MVVAFSRGDLATAIRINAEVSPLYRAIFSNTNPIMIKAACNLVGMKAGGLRLPLVQAS
                     PAEVKMMQEVLKTMGLL"
BASE COUNT         1131 a         1018 c         1233 g         1064 t
ORIGIN      
        1 tatctgaata acgctctatg aggaatacca tacgaatgta attttgttac ccggtagcgg
       61 cgtgactgtt cgcggctagt gccgattctg aaaccccggc ggaaaaaact tcacctccgt
      121 tcaaaatgcc gaatatacta cattaacact aaccgatcct tccatgggat ggaatgtcaa
      181 gaaatcggcg cagtgtaggt gggttatgcg aggcatgcaa agagggggag acgtttcgtt
      241 ggagcgtatt tccgatctac aggcagccgt aattggggga gacactcgcc aagtcgcggt
      301 agcggaagct ttggctaatg aatttaaaac agtaaaatta tatggccata tagcggcgga
      361 aactccgacg gctgttttaa acaccgcgag tctgcttgag acgcttagtg aggtccgagt
      421 tgtcgtatta ccgatctccg gaatgaacgt ggttggaatg gttcgcggcc ggaagatcgg
      481 ggaagagatt gattttggaa gttgtctaaa tgcgttaagc ccaggaacgt tgattgtgac
      541 cggttcgctc acttcggatt ggttacagag atgctcggag ctgcggctgc aaatcgtcca
      601 gtatgccgag gatgacgaga tcgccatcct gaattcaatc cctacagctg aaggggcgct
      661 gcagatagct ctggaagaga tgcccatcac catccatgac agcacggtat tggttttagg
      721 atttggaagg gtgggaatga cggttgcccg cacttttaaa gccctaggtg cccgcgttaa
      781 agtcgccgca aggcgaaatg acgtcttggc acgcgcgtgg gaactgggat gcgatcggat
      841 acctttatcg accgcttcaa cgcttgggat ggagaccttg aatgaatcca tcgcgaacat
      901 cgatctcatc ctcaacacca tccccgcctt aatcatggat tcgactttac ttgccaaaat
      961 ctcgtccagg acactcatta ttgatttggc ttcgccgccg ggcgggacgg attttgaagc
     1021 agcaaaacaa ttccatattc gagcgatttt agcacccggg ctaccgggaa aggtggcacc
     1081 gcaaaccgcg ggagcgattc tggtggaaac catccccagg ttgatcagga attcattata
     1141 cgggggtgat cgttgatgct ctccggggtg aagatcggct tcgccattac cgggtctcac
     1201 tgtaccctcg gcgaagtctt gcctgcaatg caacagttaa aggaggaagg ggcggaactc
     1261 ttcccgattc tttctccatc ggttcgggat acggacacac gtttcggcaa agcggccgat
     1321 ctttaccaaa aagttgtgga gatctcgggc catcctccga tcactacaat tgccgaagct
     1381 gaaccgattg ggcccagaaa aatgctggat ctggttctgg tggcaccatg tacgggaaat
     1441 accatggcca aattggcgca gggaatctcc gatacttccg tcaccatggc ctgcaaggcg
     1501 caattacgca acaaccggcc ggtggtgttg gccatcgcca caaatgacgg tttagggact
     1561 aatgccagga atttggctac tttattgaat aaacgccata tttactttgt gccctttggt
     1621 caggacgccc cgcataccaa ggaaacttcc ttgaatgcca ggatgagttt gatccctgat
     1681 acaatagcgt cagccctgga acaacggcaa atacaacctt tactgctggt ttttcatgag
     1741 gcgtgaggtg agaggcatga ggcaagaggt taaagtcgga tgtcgtaaag tgaaaaaaag
     1801 ttacagggat ttccaacttg ggaatttgga ctttagacta gaataaaaga cttacaatac
     1861 tcaatgcctc gcgcctcttg acttaattga cttccgactt tagacttggg actcaagaga
     1921 actcatgcct cgtgcctcat gccggttgcc ttgatgactt tagacgcaaa acaaaggatc
     1981 aacttaattg atagcgaatt ctacggcata aaatgagcgg tttcgaaaca actttggtat
     2041 cttcaattta gcaattatcc atgaaagcga ggctctttat atgcgaatca ttgtacaaaa
     2101 attcggcgga acttcggtgg caaccccgga aggcagggag aatgtcgtta aaaaagtaag
     2161 cgaggcgctg aaacagagct atcgggttgt ggtggttgta tcggccatgg gccgggcagg
     2221 tgagccttat gctaccgata ccttaattgg cctgatccga agtataaaag ctcaggaaag
     2281 tcccagagat ttggatcttt tgatgtcctg cggcgagaat atctcgactg tggtcatggt
     2341 acaaacctta aaggctcatg gcattgaggc tcaagcgctc accggcggcc aggcgggtat
     2401 tctcaccgac gggcagtttg gtaatgcgcg tatcttggag ataaagcccg ataagattat
     2461 gaaatgtttc gatgaagggc gggtcgcggt ggtcgccgga tttcagggta ctactgtgga
     2521 cggcgctgtg acgaccttag gtcggggcgg aagcgataca accgcggcag ccttgggggt
     2581 tgctcttaag gcagaagtcg ttgagatctt taccgatgtt gacggggtaa tgaccgctga
     2641 cccccggttg gttccggagg ctaagcccct tgcaaccatg acctataacg aagtttgcga
     2701 tatggctcat cttggggcca aggtggtaca ccccagggct gtggagatcg ccatggaagg
     2761 ccggataccg ctgcggatcc gctccacttt ctccgagaat ctggggacgc tgatctgtga
     2821 tggtagcccc atcggcaacg tcgagatccg gagcgacaag gtcgtaaccg gcatcgccca
     2881 tatccagggt atggcactgg tcaaactcaa gtccaaggag gatttgaata aaaccggaca
     2941 ggttttggaa atctttcaat tgatggctaa agctggaata agtgtagata tgattcaagt
     3001 cgcacctgca aatgtcagct ttattattaa agaagatcag atcgggcagg ccaaggaggt
     3061 tctagatcaa ttatcgcttg agacttccat ggagaaaggt ttcgccaaag tggccattgt
     3121 cggatcagga atgcgtggcg ttcccggtgt aatggcgcgg atggtacatg gtttacaaca
     3181 ggccgggatc tccatctacc attcgacgga ttcccatgtc agtatcgcct gtctggtgcg
     3241 acaggatgaa atgatcaaag ccctccgggc tttgcacgaa gagtttgagt tgggtagtaa
     3301 ggaggaaacc aatggttaag ttcggaaaag tgttgacggc gatgattacg ccgttcgatc
     3361 ccaatctgca ggtcgactac agggcagctc aagaattggc ggtacgtctc attcagtccg
     3421 gctccgacgg tgttgtggtt gccggtacga ccggggagtc gcccaccctg accaaaacag
     3481 agaagcttac actcttcaaa gcggtggtcg aagctgtcgg gggcgaaggg attgtcatcg
     3541 ccggtaccgg cagttacaac acagcagata gtattgagct gaccaaagag gcggaacgaa
     3601 cgggcgttga cggggttatg ctagtggctc cgtactataa taagccatcg caggaaggat
     3661 tgtaccaaca ctttgccgct atcgcccggg aaaccgcact cccggtaatc gtttataata
     3721 taccgggccg gaccggagtc aacatcactg cggataccat ggtaagattg actgaaatat
     3781 ccaatatcaa tgctgtgaaa gaagcatccg ggaacctgaa tcaggttagt gaaatctatg
     3841 aaaaagcccc gcgggatttt cagatttata gcggcgatga tgctttaacc ctacctgtaa
     3901 tgagcctcgg cggggtcggt gtcatcagtg tggccagcca tatcgtcggc cgtaagctcc
     3961 aggagatggt ggttgccttc tctcggggcg atttggcaac ggctatccgt atcaatgccg
     4021 aggtgtctcc cttgtaccgg gcaatcttta gtaacaccaa tccgattatg attaaggccg
     4081 cgtgcaacct ggtgggaatg aaggccggcg gcttgcggct tcctttagtt caggcttcac
     4141 cggcagaggt taaaatgatg caggaagttt taaagaccat ggggctcctt tagtcaaaac
     4201 cggatcctaa aaatcgggga tcttggctag ctcggttcga atttgcaaaa ttcggcaaaa
     4261 ccacttataa tgagtaacag acaagggtta taacgctttt tacaccaacc tatcttgagt
     4321 tgggaacgga ggttttacca atgttttccg gcattatcga tcccaatgcg gagatcggac
     4381 tcaatgttga aattggcgaa tttgcagtga tcggtgctgg agtgaaaatt gaaccgggct
     4441 gccgta
//
LOCUS       DASVMB010000109        14110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_13478,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000109 DASVMB010000000
VERSION     DASVMB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13478"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..972)
                     /locus_tag="VF531_03510"
     CDS             complement(166..972)
                     /locus_tag="VF531_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713065.1"
                     /translation="MNGAGSSGRERIKSKTLRQTLFILALLTLWELLARVGQLPALLF
                     PPISSVLQELVVQTMNGELIQRTGFSLYLIGTGLGLAVISALILTAFGVSSQIFGEWV
                     QVLMAVFHPLPGIAILPVIILWLGTGAQSIVAVIWLSAVWPMVANLNTGLTSIPATQI
                     EVGRNLGLGRFALIRSVLLPGAFPHILSGLRVGWARAWQAAVAAEMVFGACGGEGGLG
                     WFIYKKRFFMEIPDVFAGMIVIVLIGLLVERMGFEWIETRTVKRWGMSLS"
     gene            complement(1009..1944)
                     /locus_tag="VF531_03515"
     CDS             complement(1009..1944)
                     /locus_tag="VF531_03515"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7713066.1"
                     /translation="MTVGVFIVNQLPNAAAKSLPEIKIAGQFGLVYAPLMVADKLQIF
                     ERYGLKPVWREYGSGAAVREALISGEADVGFMGIPPFLIGWEKGCPWKIAMGFVIVPV
                     GLVTNDPAIKSLKNFKPGDKIAVPSPGSVQHILLSMAAKKELGSANALDNNLIAMAHP
                     DATAALISKKGLTAHFTTPPYLFEESIQPGFHQVTNDIAIFGSPFSFNVGVASQRFHD
                     QKKDEYHRFVKAIDASMTEITKNPRKVAELLAPEFKLTPEKTYQYMTWPGMNYTTKLY
                     GVMGFAEFMKEAGFIKKAPQNLSEIIWENVVVGGK"
     gene            2245..3180
                     /gene="cysK"
                     /locus_tag="VF531_03520"
     CDS             2245..3180
                     /gene="cysK"
                     /locus_tag="VF531_03520"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7713067.1"
                     /translation="MTKIAKSLTELIGNTPLLELSNYNRDEKLQAAVIAKLEYFNPGG
                     SVKDRIGYAMIKDAEERGLIRPDSVIIEPTSGNTGIALAFVAATKGYRLILTMPETMS
                     IERRNLLQALGAELVLTPGPEGMKGAIRKAEELAATLPHSYIPQQFNNPANPEIHRRT
                     TAEEIWRDTGGGVDVFVAGVGTGGTVTGVGEILKKKKPGVRIIAVEPYESPVLSGGNP
                     GPHQIQGIGAGFVPEVFDRSVIDEIIKVKGDEAYRTSRKLAKTEGLLVGISSGAAVFA
                     ATQIAKNPENKGKNIIALLPDNGERYLSTDLFQES"
     gene            3296..3739
                     /locus_tag="VF531_03525"
     CDS             3296..3739
                     /locus_tag="VF531_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7713068.1"
                     /translation="MMDGMTYYSALPSRILEYIHDETRDSLYYQELAKKAPDERAKDL
                     YLEFSRDEATHAANFCQVYIRLTGMQPVTPPVEPPEIPPYCDAVKQRIMAESGDFVKY
                     GQEFINAPDCELRDLFYITGAIEARHGMRLATLLCGVEGGRRDCQ"
     gene            4000..6408
                     /locus_tag="VF531_03530"
     CDS             4000..6408
                     /locus_tag="VF531_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713069.1"
                     /translation="MRYTKYLMMDAKSAELPQPKISRPDLSTALNEAVRLSDSHSYDA
                     ALVQYEALIGQFSDVKDTPILAYLHYQRGISLKKLALLTDGQANLVKAAAAFESAAQL
                     YSHPQNLLGLAQAQAGLGSVWANLSEYQSKSQHLAKAMQFLEETITTLEKGVKAFNSK
                     QHPQDYALLELISAMTHNALAEYKDKGTHLTLAVAAGEEAMAVFSRSDYPSLHALSQF
                     NLGYAHHVLAELNDPAENRERAYGAYQAALEIYNSDEYPFEYAHTHNCLANLYIDRAA
                     AANREEDLNQAINSCKEVLNVLNSVNQYPLSYSYILHNLGSACKLFARIRGGGQYLEK
                     ARQYFQEILRIENLPPASEILGSTYLNLGVILQQSAEGAERHLPEAVGMYEKALTIYT
                     AADFPRQYAITQSNFGSACLALADFDARRNWLFRAVQAFTEATRTFTVAAAPQDHAAS
                     QVKLGDAYIELAREDYQKEYPTAAIAAYEAALKIFRPESHRSAHVHAAIQLGKACKFL
                     AEHEPSTATLNKTLQVYENTLRLLNPSEQRVEYAETLARLGEAYYSFAEFGTKEINLT
                     RALKCFQDSVKLYGNEYPSEYAYFNVELGLAALGLAELQPKTHYPGVAVRALNEALNF
                     YSYEKNPERFAAIQNAIGRAHLIWATVENRQENLEKALAAFSAALKCYNPDRFPVEFG
                     TNMNYLGSTCKAMAEEMQSMNYKSLDEYKKYKMERIFNAISVFQGALTIFTVDHYPEQ
                     YAQTQTNLGEVFALLAMLQEPQENLARAISALESALLVYTPENYPAQYRKVMNHLERL
                     RQQL"
     gene            6534..6824
                     /locus_tag="VF531_03535"
     CDS             6534..6824
                     /locus_tag="VF531_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713070.1"
                     /translation="MLDPQLSNGLESIKQIVEHLHRVEQDNAALASQLAQEEYSIAAD
                     LNRNLGNVPALAANARQAAAVFSRLAADGTHAAEELAKLNEVIVQFQNEHGG"
     gene            6987..8195
                     /locus_tag="VF531_03540"
     CDS             6987..8195
                     /locus_tag="VF531_03540"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX7713071.1"
                     /translation="MTQSSAVPFEDYDDLTLAPYAAKSKTSRGRRFSESEDTYRNCYQ
                     RDRDRIIYSTAFKNLQYKTQVFVVHEGDFYRTRMTHTLEVMQHARTFARVLRSNEDLV
                     EAIALAHDLGHTPFGHAGEEILSELLQKYGGFDHNLHSLRVVDRLEQRYPQFSGLNLT
                     YETREGLARHLTRFDNPKLPVEFATSPQPGIEAQIVNLADSMAFAAHDLDDALRIKLV
                     DWEDLQNLPIPSVRDILNMIGEEERQLGGFTPQMRLYRFIRFWIYHFNEDCIIQTRRN
                     LEAIRPDSSQAIRELSEPVVTLSPERTNDLQMLINFLYNQVYKHPLVLMMAEKGKMIL
                     RRLFEQFMSNPRLLPVMIREKCDNSEDESNRALQIADFLAGLTDRQAMDIYEMMFEPY
                     TKVMGYGFNK"
     gene            8229..9071
                     /locus_tag="VF531_03545"
     CDS             8229..9071
                     /locus_tag="VF531_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7713072.1"
                     /translation="MKQRIDQWLVEQGHFTSREQAQRAVMAGEVRLDGKPVVKPSQPV
                     PDVHRDQPIQVTIVGTGQPYVSRGGLKLEKALRTFAVSVAGKICLDAGASTGGFTDCL
                     LQSGAVKVYAIDVGYGQLAWKLRQDSRVILWERENIRYFEVRHLDSLPELITADLSFI
                     SLELVFPKFQEMLAPGGDVITLIKPQFEAGRDKVGKKGVVRDRAIHLEILKRLANRLP
                     QSGWQLVNLTFSPIRGPEGNIEYLGHWRRMNHGIEITPQSGEMIETVVTSAWEELDHS
                     QGES"
     gene            9158..10315
                     /locus_tag="VF531_03550"
     CDS             9158..10315
                     /locus_tag="VF531_03550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7713073.1"
                     /translation="MVPVQTFEMTTKVIFGIESLRELPTIGRTLGKKVLVVTGKTALI
                     DNGVLHRVETLLNGAGFQMTAFSEVEPEPSLDTVRKGLALGRSIQADWVIGLGGGSAM
                     DVAKAIAGLFGGENELEFYFDGGKIMQPGIPLVTIPTTSGSGAEVTHFAVLSDRVRKI
                     KRPIEGNSFAACIAVIDPQLSMSTPDQVTIYSGLDALTQGIEAFTSKDSTALSDIYAF
                     SAVERISNNLLNVYHDGQDIKARTEMTLGSLESGIALRNAGMGAVHGLAHPIGIRTGK
                     PHGLVCAVLLLPVMRFNLSACYEKYARITKAMGLNTVGGSDTIDVAALSMKHILSLER
                     KLGIPTHISGLGITEAELPQIVAEALTTPAMKSNPREATADDLLNILRENF"
     gene            10315..13149
                     /locus_tag="VF531_03555"
     CDS             10315..13149
                     /locus_tag="VF531_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7713074.1"
                     /translation="MRSDHRYLYSFILLFICFWGITAQGVDGIGLAGNSREVAGQAEN
                     GSSKSGGAPGVALYESLQTLVRTHPGDVKSRIRLVRLMYSQSGLPEYLATRSLLWAEN
                     NSRSEPLRGKLPAVNPALVSECQKQLLRILDQDPRNSEALLMAGNCQLFVEQKQNALW
                     YFHQALTDASDNQARLALADYYLQEWQPAEALQVVANETGPEIALRKGIAYLELNDYP
                     MAYGFLLEAAPLPQAYRWTREKDLFKASLALGVTDRVREYKKLGLPPEPMAATLFLEL
                     KGWSAWLDGNSEEALRVWNMGRALSPDYWVWEGAIWYLPEKPGVAADFPKGWNDRYLD
                     SNLRLRQGDAYLQEGRSTQAYQSYLAAIKSDHRSLIGFLKAGEVQLKAKAYTKAIDLF
                     SQALAVNPGFAPILLERATAYQRSGHSDLAAADRKAAGQALTGVMDGSIQAQLFDDGR
                     NKVELILRGATRNLTGIWVYDGKTEWKWLPWWGSPIYLNGLFKQLWVVPVGPGLSGLA
                     LFLEKNSSPTPEPAIQVVMRDDGLWLESSFATRMVGEIIQNNSTRRIVLFDQLVNEGH
                     IPWDLFPQGQSELGVGWQETSGAWRTAQYSLIRPALPSSTDSDTPGNNPEPPIQESSG
                     TVGNRWDGGAADSVGADGPDFAIDYPKLLSIAVGRNGQRRWKLNWTVESGVVSSLEIL
                     TDTGIWKRIIPTGEQGRLRADIESIAAVFCRIRLENTAGRVSFYPVGQINEAWRSASS
                     EAFVINDGTGLINSRSVQVSPVNQSDDLEWGISNDLRIWSPWHQGSAPVKWRMSSRDG
                     SILLFVRYRYRSTPQFQRITVVGTTLDTVPPELRNLDWRKSGSDFLIDLQFDEAVQIQ
                     AVLTDISGQTIDSRDPADIYQSAVSLVFPLDRVSPGGQIQFLVRDQAGNTCSLRYGIS
                     NDGLEALKDESFSPAPSPPVTGKEGRRWIY"
     gene            13345..>14110
                     /gene="speE"
                     /locus_tag="VF531_03560"
     CDS             13345..>14110
                     /gene="speE"
                     /locus_tag="VF531_03560"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX7713075.1"
                     /translation="MELWFTEKQTPNLGLSCKITQTLHTEKTEYQDLAVIDTVQYGRM
                     LVLDGMVMTTEVDEFVYHEMISHVPLNTHPGPERVLVVGGGDGGAIREILKHPSVKEA
                     VLAEIDPRVVEVSQKYLPSIAGSLKDPRVTLAIGDGIAHVSQNKNRYDVILVDSTEPI
                     GPAVGLFSRRFYQDLYEALTPEGIMVAQSESPFINPDVIQMIHTNLTGIFPLKYLYTV
                     SIPTYPSGLWSFTIASKKWDPLRVDPEKIRQNRFTTK"
BASE COUNT         3545 a         3392 c         3774 g         3399 t
ORIGIN      
        1 cagcccgatg gccccagtat ggtcaggaaa ctccccgctt caagtgtaaa gtcaagtgcc
       61 tccagaacct ttgtatcctt gccgtttacc ttaaattgtt tcgtaagccc ggtgacggac
      121 aacagcctgg tgacagataa atcttccacg gcgccgcacc tcccgtcagc ttaaactcat
      181 gccccagcgt ttcacggtcc gggtttcaat ccactcaaaa cccatccgtt ccaccagcaa
      241 accgatgaga acaatgacga tcattccggc gaaaacatcg ggtatctcca tgaagaaacg
      301 tttcttgtag atgaaccaac ccaacccgcc ttctccgcca caagcgccga agaccatctc
      361 tgccgcaacc gcagcttgcc atgcccgcgc ccaacccaca cggaggccgc ttaggatatg
      421 agggaatgcc cccggtaaaa gcaccgaacg gatgagagca aatcttccca gccccaggtt
      481 ccgtcccacc tcgatttgag tggcggggat cgaagttaag ccagtgttta gattggcaac
      541 catcggccag accgcagaga gccagattac agcgacgatc gattgagcgc cggtaccgag
      601 ccaaaggatg atcaccggca gaatggcaat gcccggtaga gggtgaaaaa ccgccattaa
      661 aacctggacc cattctccga agatttggga gcttaccccg aatgcagtca agattagcgc
      721 cgaaataacg gcgagcccca gtccggtacc gatcagatag agagagaatc cggtccgctg
      781 aatcaattcg ccattcatgg tttggaccac cagttcctgt agaacactgc tgatgggggg
      841 aaacagtaag gccgggagct gcccgaccct ggcgagcagt tcccagaggg tgagtaaagc
      901 tagaataaaa agggtttgcc gcagagtttt tgattttatg cgttcgcgcc cggaacttcc
      961 ggcaccgttc atcgcagatc cctcgcttga tttgggcagt aagaagagtt attttccacc
     1021 gacgactaca ttttcccaga tgatctccga tagattttgg ggcgccttct tgatgaaacc
     1081 ggcttccttc ataaattcag cgaaacccat gacgccatat agtttggtgg tatagttcat
     1141 accgggccag gtcatatact ggtatgtttt ttccggagtc aatttgaact ccggagccaa
     1201 aagttcggcg actttccggg ggtttttagt gatctcagtc attgaggcgt caatcgcctt
     1261 cacaaagcga tggtattcgt cctttttttg gtcgtggaat ctttgcgagg ctacaccgac
     1321 attgaaactg aatggcgacc cgaaaatggc gatgtcgttc gtgacctgat ggaatccagg
     1381 ctgaatcgat tcttcaaaaa gataaggcgg ggtggtaaag tgagcggtca atcccttctt
     1441 ggatattaaa gctgcagtgg catcgggatg cgccatggcg atgaggttgt tatcgagggc
     1501 attggcggaa cccagttcct tcttggccgc cattgagagc aagatgtgtt gtacgctgcc
     1561 gggcgaaggt accgcaatct tgtcgccggg cttaaaattt ttgagactct taatggcggg
     1621 atcattcgtc accagcccta ccggtacaat gacgaatccc attgcaatct tccagggaca
     1681 acctttctcc cagccaatca ggaatggtgg aatccccata aatcccacat cggcctcacc
     1741 cgatattaac gcctcgcgga ctgcggcgcc ggatccgtac tcacgccata ccggttttaa
     1801 tccatatctt tcgaagatct gcaatttatc ggcgaccatc aatggtgcgt aaaccaggcc
     1861 gaactgtccg gcgatcttga tttccggaag cgatttggcg gcggcgttgg gaagttggtt
     1921 tacgataaat actccaaccg tcaaaaggaa cagacaacaa atgaaccgaa tcttttttat
     1981 gatggacctc tccccctttg aaatgaaatt taggacttgg gaactagata atatagggta
     2041 tgcagtaagt gcggggtttg gtgtctcatt accagtattc ctatcattaa aatatggatt
     2101 actcataacc ttagggaagg agccattctt catgtaatgt aataagtcag aaaatattga
     2161 caatcatagg gatggggagt aggataataa tattctacca agataatagg tttacctgga
     2221 tttcgaagag atggaggaag gatcatgacg aaaatcgcca agagtctcac ggaactaatc
     2281 ggcaataccc cgcttttgga gttatccaac tataacaggg acgaaaaatt acaggccgcg
     2341 gtcatcgcca agttggagta ttttaacccc ggcggtagcg taaaggatcg gatcgggtac
     2401 gccatgatca aagatgccga ggagcgaggg ttgattaggc cggactcggt tatcatcgaa
     2461 cctaccagtg gtaataccgg aatcgcattg gcctttgtgg ccgcaaccaa ggggtaccgt
     2521 ttgatattga cgatgcctga gacaatgagt atcgaacggc gaaatttact tcaggcgctc
     2581 ggcgcggaac tggtattaac tcccggccct gaggggatga aaggcgcgat acgcaaggcc
     2641 gaggaactgg cggcgacgct gccccattcc tacatccctc aacaatttaa caacccggcc
     2701 aatccggaga ttcaccgccg gacaacagcc gaagaaattt ggcgggatac aggaggcgga
     2761 gtggatgtat ttgtggccgg agtcggaacc ggcggcactg tgaccggagt cggggagatt
     2821 cttaaaaaaa agaagcccgg agtacggatt atcgctgtgg aaccctacga gtcaccggtg
     2881 ctttcgggcg gcaatcccgg acctcatcag attcagggga ttggagccgg gtttgttccg
     2941 gaagttttcg accgttctgt cattgacgag atcatcaagg ttaaagggga tgaggcttat
     3001 cggacctccc ggaaacttgc aaaaactgaa ggcctgcttg tgggaatttc ctcgggggct
     3061 gcagtttttg ctgcgactca aatcgccaag aacccggaga ataagggaaa gaatattatc
     3121 gcgttattgc cggataacgg tgaacggtac ctttccaccg atctctttca ggagagttaa
     3181 gccggactta taaaagttta tcgaatcata accgaccaca tctttttaaa aaataatgtg
     3241 attgcagtca gcactccatc ctgttgcata ggatatcttt ggaggtgctg atggaatgat
     3301 ggatggaatg acttattatt cggctttgcc gtcccggatc ctggaataca ttcatgatga
     3361 gacccgggac tccctttatt atcaggaact cgccaagaaa gccccggatg agcgggcaaa
     3421 ggatttgtat ctggaatttt cacgtgatga ggcgacgcat gccgccaatt tctgccaggt
     3481 ctacatcaga cttaccggga tgcaacccgt tactccgccg gtagagccac ccgaaattcc
     3541 accttattgc gatgcggtaa aacaaagaat catggcggag agtggggatt ttgtcaaata
     3601 cggtcaggaa ttcatcaatg ctccggactg tgaattgcgg gatctttttt atattaccgg
     3661 ggcgattgaa gcccggcatg gaatgcggct cgccacctta ctatgcggtg tggagggtgg
     3721 ccgccgtgat tgccagtaga tggactcacg gacgcgcaac catggagtcg aggagacata
     3781 aagcaggctt aaaaacgctc taccgataat attaagtatt taaaaaagta cccggtgcat
     3841 cattcaggaa gccgggattt ttttatggta tttggttatc tgaaagttaa cttttctctt
     3901 aaaatcggaa gtttggaaaa ggatttttgt agtttggcat ccaatatttt aaatgaatga
     3961 tcttgcgaaa cttaaaaagc ctggtgacct ttatttacca tgagatacac taaatacctg
     4021 atgatggatg caaaaagcgc ggagttgccg caacccaaaa ttagcaggcc cgatctgtca
     4081 acagccctga atgaagcggt ccggctctcc gacagtcatt cgtacgatgc tgctttagtt
     4141 caatatgaag ctttaattgg ccagttttcc gatgtaaaag atacgccgat tttggcttat
     4201 ctccattacc aacgcggtat atctctaaaa aaactggcct tgctcaccga tggtcaggct
     4261 aatctggtca aagccgccgc agcgttcgaa tcagccgccc aattatattc ccatccccaa
     4321 aacctgttag gtctggcaca agctcaggcc gggctcggtt ccgtatgggc caatctttcc
     4381 gagtatcaat ccaagagcca acatctggcc aaggccatgc aattcctgga ggaaacgata
     4441 accaccctgg agaagggggt taaagcgttc aattccaaac aacatccaca ggattacgcc
     4501 ctactcgagc ttatttcggc aatgacccac aacgcattgg cggagtacaa ggataaggga
     4561 acccatctta ccctggcggt cgcagccgga gaagaggcga tggctgtctt ttcgaggtcc
     4621 gattacccgt cactccatgc tttaagtcag tttaacctgg gttacgccca tcatgttctg
     4681 gcggaattga atgatcctgc cgaaaatcgt gaacgggcgt atggagccta tcaagctgcg
     4741 ttggaaatct ataacagtga cgaatatccc ttcgaatatg cccacaccca taactgcctg
     4801 gcaaatctgt atattgatcg tgccgcagcg gctaaccggg aagaggattt gaaccaggcc
     4861 atcaactcct gcaaagaggt cctgaatgtc ctgaactcag tgaatcaata tccattgagt
     4921 tacagttata tccttcacaa tcttggctcg gcatgcaaac ttttcgcacg aatccgcggc
     4981 ggcgggcagt acctggagaa ggccagacaa tactttcagg aaattctgcg gatcgaaaac
     5041 ctcccacccg cctcggagat tctcgggtcc acctatctaa atttaggggt gatcttgcag
     5101 caatcggcgg aaggggcgga acgccatctt cctgaagcgg tcggaatgta tgagaaggca
     5161 ctcacgattt ataccgctgc cgattttcct cgtcaatacg cgattaccca aagcaatttt
     5221 ggatccgcct gcctggcact ggcggatttc gatgcccgcc ggaattggct cttccgggcg
     5281 gttcaagcct ttaccgaggc gacccggacc tttactgtgg ccgcagcacc gcaggatcat
     5341 gccgcaagcc aagtcaagct gggtgacgct tatattgaat tggcccggga agactatcaa
     5401 aaggagtacc ctaccgcggc gattgcagct tatgaagcgg cgttaaaaat tttcagaccg
     5461 gaatcgcatc gttccgccca tgtccacgcg gccattcagt taggtaaagc ctgtaagttt
     5521 ctggccgagc atgagccttc caccgccaca ctcaacaaaa cgcttcaggt ttacgaaaat
     5581 actctacggc ttttgaaccc atccgaacag cgggtggaat acgccgagac cttggctcgt
     5641 cttggggagg cgtattattc attcgcggaa ttcggcacca aggagatcaa tctgacccgg
     5701 gcgcttaaat gttttcagga ttcggtcaag ctatatggaa acgaatatcc gtccgaatat
     5761 gcttatttca atgtggagtt gggtttggcc gccctgggcc ttgccgagct tcaacccaaa
     5821 acccattatc cgggagttgc agtgcgggca ctcaatgagg cattgaactt ttatagctac
     5881 gaaaagaacc cggaacgttt tgcggcgatt caaaatgcca ttggtcgtgc tcatcttatc
     5941 tgggctacgg ttgagaaccg tcaagagaac ctggagaagg cattggctgc ctttagcgcc
     6001 gctttgaaat gttacaatcc cgaccggttt ccggtggaat tcgggacgaa tatgaattat
     6061 ctcggcagca cttgtaaggc tatggctgaa gagatgcaga gcatgaacta caagagcctc
     6121 gatgaatata agaagtataa aatggaacgg atattcaacg ccatcagcgt ttttcaaggc
     6181 gcattgacta tcttcaccgt tgatcactat ccggagcagt acgctcaaac ccaaacgaat
     6241 ttgggcgagg tctttgcatt actggcgatg cttcaagagc cccaggaaaa tttggcccgg
     6301 gcgatctcgg cactggaaag cgcactactt gtatataccc ccgagaatta cccggctcaa
     6361 tatcgaaaag tcatgaatca tctggaacgg ttaaggcagc agctgtagaa aataagaacc
     6421 tgagattttt tactgcggcc tccctgaggc cgttttttta tgaaacattg ttatggtata
     6481 aaacccccgg ttacggggaa cccctacctc caaaaggaaa tgggggtaaa aagatgttag
     6541 acccgcagct atcaaatggt ttagagagca taaaacagat cgtcgaacat ttacaccggg
     6601 tggaacagga taacgccgcc ctcgcctcgc aactcgctca ggaagagtat tcaattgcgg
     6661 ctgacctcaa tcgtaacctg ggaaatgttc cggcgctggc cgctaacgct cgtcaagccg
     6721 cggcggtttt tagccggctt gccgcggatg gcacccatgc cgcggaagaa ttggcaaaac
     6781 tgaatgaagt cattgtccaa ttccaaaatg agcatggggg ttaaggatct tcttaccagg
     6841 ctcttgccta tcctaaaaaa gcgatttacc ggcaggatgc catccaattc tgtcgaaagg
     6901 aataacgatt agaatttcta aggcttttag tttacccgga ccggatgaac ctccagttcc
     6961 gggtgacgat gagcgaggtg agttgcgtga cgcagtcttc cgcagtcccg tttgaggatt
     7021 atgatgatct aactttagct ccttacgccg ccaaatcgaa aacctccagg gggaggaggt
     7081 tttccgaatc cgaggatacg taccgtaatt gttaccagag ggatcgcgac cgcatcatat
     7141 actctacggc tttcaaaaat ttgcagtaca aaacgcaagt ctttgttgtt catgaagggg
     7201 atttttaccg gacccggatg actcacaccc tggaagtgat gcaacatgcc aggacttttg
     7261 ccagagtgct caggtccaat gaagatctgg tagaagcgat agcccttgcc catgatttgg
     7321 ggcatacccc ttttggtcat gccggagaag aaatcctttc cgaattgctt cagaaatatg
     7381 gaggattcga tcacaatctg cacagtttac gggtggtcga ccgtttggaa cagcggtatc
     7441 cgcaattctc cggattgaac ctcacttacg agacgcggga agggctggcg cggcatctca
     7501 cccgatttga taatcccaag ttgccggtgg aatttgcgac ttcaccccag ccgggaatcg
     7561 aagctcagat agtcaatctc gcagattcca tggctttcgc cgctcatgat ctggatgatg
     7621 ctctccgtat caagctggtt gactgggaag atctacagaa tcttccaatc ccctcggtcc
     7681 gggatattct gaatatgatt ggcgaagagg agaggcagct gggaggcttc actccacaga
     7741 tgcggttata tcgtttcatc cggttttgga tctatcattt caacgaggat tgcatcattc
     7801 aaacccgccg gaatcttgaa gcgatccggc ctgattcgtc ccaggccata cgggaactgt
     7861 ccgaaccggt agtaaccttg tctccggagc ggaccaacga tttacagatg ctcatcaatt
     7921 ttctttacaa ccaggtatac aagcacccct tggtgttaat gatggctgaa aagggcaaaa
     7981 tgatactgag gcgtttgttt gaacaattta tgagtaatcc taggcttttg ccggtaatga
     8041 tccgcgaaaa atgcgacaac agcgaagatg agtcgaatcg cgcgttgcaa atcgcggatt
     8101 ttctagcggg gcttaccgat cgccaggcga tggatattta tgagatgatg ttcgagcctt
     8161 ataccaaagt gatgggttat ggtttcaaca aatagtcggg gtgtaggtgg ccgattgcgg
     8221 aggtttcctt gaaacaacga atcgatcaat ggctggttga gcaggggcat ttcaccagcc
     8281 gggaacaggc gcaacgggca gttatggccg gagaggtacg tctcgacggt aaaccggttg
     8341 tcaaaccaag ccagccggta ccggatgtac atcgggatca accgatccag gttacaattg
     8401 tcggcactgg ccagccctac gtgagccgtg gcggattgaa acttgaaaag gccttacgaa
     8461 cttttgccgt ttcggttgca ggaaaaatct gcctggatgc cggagcctcg acaggcggct
     8521 ttaccgattg cctgctgcaa tccggcgctg tgaaggttta cgccattgat gtcggatacg
     8581 gacaactggc ctggaaatta aggcaggatt cacgggtgat tctctgggaa cgtgagaaca
     8641 tccggtattt tgaagtacga cacttggatt cgctcccgga attgatcacc gccgacctct
     8701 ctttcatctc tcttgagctg gtctttccca agttccaaga gatgctggcc ccgggtggag
     8761 atgtaatcac cctgattaaa ccacagtttg aggcgggacg tgataaagtc ggtaagaaag
     8821 gcgtggtccg cgaccgtgcc attcatctgg agatcttgaa acgcctcgcc aatcgtctgc
     8881 ctcaatccgg atggcagttg gtgaatttga ctttttcacc gattcggggt ccggagggaa
     8941 atatcgagta tttgggtcat tggcggcgta tgaatcatgg tattgagatc actccgcaat
     9001 ccggagaaat gattgagacc gttgtgacct ccgcctggga ggaattggac cattcccaag
     9061 gagaatccta gcttcttggg aaggcttagt ttagcccaat cccttagcgg gtttataaac
     9121 ggttaagatt gtagcctcaa tgaaggagag cggtaatatg gtacctgttc aaacctttga
     9181 aatgacaacg aaagtcatct tcggtatcga gtcattgcgt gaattaccga cgatcggaag
     9241 aacgcttggt aaaaaggtac tggtggttac gggaaaaacc gctttgatcg ataacggtgt
     9301 gcttcaccgg gtggagacac tcttaaatgg ggcgggcttt cagatgaccg ctttttctga
     9361 agtcgaaccg gagccttcgc tggatacggt ccgaaagggt ctggctttgg gtcggagtat
     9421 tcaagcggat tgggtcatcg gtttgggtgg cggtagtgcc atggatgttg ccaaagccat
     9481 tgcgggatta ttcggcggcg aaaacgagct ggaattctat ttcgacgggg gtaaaatcat
     9541 gcagcccggg atcccgttgg ttactatccc taccacttcc ggttccgggg ctgaagtgac
     9601 tcattttgcc gtgctctccg accgagtacg caagatcaaa cggccgattg aagggaacag
     9661 ctttgcagcc tgcattgccg tcattgatcc gcaactctcc atgagtaccc cggaccaagt
     9721 caccatttac tcgggtttgg atgctttaac ccaagggatc gaggctttta cctctaaaga
     9781 ttccactgcc ctgagtgaca tttatgcctt ttccgcagta gaacggataa gcaataattt
     9841 gttaaatgtt taccatgacg gccaggatat caaggcccgc acggaaatga cgttggggag
     9901 tcttgaatcg ggtatcgctt taagaaacgc gggaatgggt gccgtccatg gcctagccca
     9961 tcccatcgga atcaggaccg ggaagcctca tggactcgtc tgtgcggttt tgttactgcc
    10021 cgtgatgcgt tttaatctct cggcatgtta cgagaagtat gccaggatca ccaaagccat
    10081 ggggttaaat accgtcggtg gaagcgatac cattgatgtg gcggccctca gcatgaaaca
    10141 tattttgagt ctggagcgga aactggggat tcctacccat atcagcggtt tggggattac
    10201 cgaagcggag ttgccgcaga ttgtggccga agctttgact acaccggcga tgaagtccaa
    10261 tccccgtgag gccacggccg acgatttatt aaatatttta agagagaatt tttaatgcga
    10321 agtgatcacc ggtatcttta tagcttcatc ttgttgttta tttgtttctg gggcataact
    10381 gcccagggcg ttgacggtat cggcctggcc ggtaacagtc gggaagtcgc cggtcaagcg
    10441 gagaatggct caagtaaaag cggtggagct cctggcgttg cactttatga atcgctgcaa
    10501 acgttggtgc gtacccatcc cggggatgta aaatcccgaa tccgcctggt gcgacttatg
    10561 tatagtcaat ccggcttacc cgaatatttg gccacccgga gcctgttatg ggcggagaac
    10621 aacagccgca gcgagccgct tcgtggaaaa ctgcctgcag taaatccagc gttggtttca
    10681 gagtgccaaa aacagcttct ccgaattttg gatcaggatc cccggaattc ggaggccttg
    10741 ttgatggctg gcaattgcca gctttttgtc gaacaaaagc aaaatgcgtt gtggtacttc
    10801 catcaagcac tgaccgatgc ttccgataac caggcccgtt tggctctggc cgattactat
    10861 ctgcaggaat ggcagccggc cgaagcctta caggtggtcg caaatgaaac cggcccggag
    10921 atcgctctgc gtaaaggtat tgcttatctg gaactcaatg attacccgat ggcttacggg
    10981 tttcttttag aagccgctcc gctgccccaa gcttaccgat ggacgcgaga gaaggatcta
    11041 ttcaaggcat ccctggcatt aggcgtaacg gatcgggttc gggaatacaa aaaactcggg
    11101 ttgccgccgg aacccatggc ggcaactctg tttttagagc tgaaaggctg gagtgcctgg
    11161 ctggatggaa actctgaaga agctctccgt gtatggaata tgggccgggc gctcagcccc
    11221 gattactggg tttgggaggg agcaatctgg tatttaccag aaaaacccgg agtggcggcg
    11281 gatttcccca agggctggaa cgatcgttat ctcgattcca acttgcggct gcgccagggt
    11341 gacgcctatt tgcaggaagg gcggagcact caagcgtatc aaagttatct cgcggcgatt
    11401 aagtcggatc accgttcgct catcggattt ttgaaagcgg gcgaagtgca attaaaggcc
    11461 aaggcgtata ccaaagccat cgaccttttt tcgcaagcgt tggctgtaaa tcccgggttt
    11521 gccccgatac ttctggaacg ggctactgca taccaacgct caggccacag cgatttggcc
    11581 gccgccgacc ggaaagcggc cggccaggcc ttgaccggag ttatggatgg aagcatccaa
    11641 gcccagttgt tcgatgatgg ccggaacaag gttgagttaa tcctgcgggg ggctacccgg
    11701 aatctgacag gtatatgggt atacgatgga aagacggaat ggaaatggtt gccgtggtgg
    11761 ggcagcccga tttatttaaa tggtttgttc aagcagttat gggtggttcc ggtggggccg
    11821 ggtttatcgg gtttggcgtt gttccttgag aaaaattctt ccccaacccc ggaacccgcc
    11881 atccaagtgg taatgcggga cgacggtctt tggctggaat cttcttttgc cacccggatg
    11941 gtgggggaga tcatccaaaa caacagcacc cggaggatag ttctcttcga ccagctcgtt
    12001 aatgaaggac atatcccttg ggatctgttt ccccagggcc aatccgaact gggggtggga
    12061 tggcaggaaa caagcggtgc ctggagaacg gcacagtatt cgttgatcag gcctgcattg
    12121 ccgtcaagta ccgattcgga cactcccgga aataacccgg aaccgccaat ccaggaatcc
    12181 tccgggactg ttggtaatag atgggatgga ggtgcggccg actcggttgg ggcggacggg
    12241 ccggatttcg caattgatta tcccaagtta ctctcgattg ccgtgggccg taacggtcaa
    12301 cgacgttgga aattaaactg gaccgtggaa tccggtgtcg tctccagcct ggagatactg
    12361 acagatacgg gtatatggaa gaggattata cctacgggtg aacagggacg cttgcgcgcg
    12421 gatattgaat ccattgccgc agtattctgc aggatacgac tggaaaacac cgccggccgg
    12481 gtaagctttt atccggtagg tcagatcaat gaggcatggc gctctgcaag ttccgaggcg
    12541 tttgtaatca atgatggaac tggcttaatt aactctcgga gtgtccaggt gagtcccgtg
    12601 aaccaatcag atgatctgga gtgggggatc agtaatgatc tacggatttg gtcgccctgg
    12661 caccagggaa gtgccccggt gaagtggcga atgagttcga gggacggttc catcttactc
    12721 tttgtgcgtt accgttatcg ttccacaccg caattccagc gaataacggt ggttgggacg
    12781 accttggata cggttccccc ggaactaaga aacctcgact ggagaaagag tgggtcggat
    12841 ttcctaattg acctgcagtt cgatgaggcg gttcagattc aggcagttct tacggacatt
    12901 tccggtcaaa ccatcgattc ccgtgacccg gcggatatct atcagtctgc agtttcatta
    12961 gtgtttccgc tcgacagggt tagtcccgga ggccaaattc aattcttggt gcgagatcaa
    13021 gccggaaaca cctgttcatt gagatatgga atcagcaatg acgggcttga ggctttgaaa
    13081 gatgagtcct tctctcccgc tccctctcct cccgtaaccg ggaaagaggg gagaaggtgg
    13141 atttattaag gacctgtctc ttgccgtcct ttggacgcgc cccccgggtg gacggaatga
    13201 caaatcggac ttttgagaca gccccgcaag aggtaggtgg attctgaaaa ttccgggaaa
    13261 gaataatatc aggttatccg tcatgtgttt cgcgaggatg ccttgacctt ttaaaccaat
    13321 tgatatcaaa ggggtaatga gcagatggaa ttatggttta ccgaaaaaca aactcccaat
    13381 ttgggactat catgcaagat cacgcaaacc ttgcatactg agaagaccga gtatcaggat
    13441 ctggcggtga tcgacacagt ccagtacgga aggatgctgg ttttggacgg gatggtgatg
    13501 accaccgagg tggatgagtt tgtatatcat gagatgatta gtcatgttcc tttgaatact
    13561 catcctggtc ccgaacgagt gttggtggtg ggcggcggcg atggaggagc tatccgggag
    13621 atcctgaagc acccgtcggt aaaagaagcg gttttggcgg agattgatcc cagagtggtc
    13681 gaggttagtc agaaatatct gccgagtatt gccgggtccc ttaaggatcc ccgtgtcact
    13741 ttggccatag gcgacggtat cgcccatgtg agtcaaaata agaaccggta cgatgtgatt
    13801 ctggtggatt caaccgagcc catcggcccg gcggtgggtt tgttcagccg ccggttttat
    13861 caggatcttt atgaagcatt gactcccgag ggaattatgg tggcgcagag cgagtctcca
    13921 ttcatcaatc ccgatgtgat ccaaatgatc cataccaatc tgacggggat ctttccgcta
    13981 aagtatttat atacagtcag tatccccaca tacccctcgg gattatggag tttcaccatt
    14041 gccagcaaaa aatgggatcc gctgcgggtt gatccggaaa aaattcggca aaaccggttt
    14101 accacaaaat
//
LOCUS       DASVMB010000110         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_160460,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000110 DASVMB010000000
VERSION     DASVMB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160460"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="era"
                     /locus_tag="VF531_03565"
     CDS             complement(<1..710)
                     /gene="era"
                     /locus_tag="VF531_03565"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7713076.1"
                     /translation="MSKTKDKKTFKSGFVVLAGRPNVGKSSMLNAFLGQKLMITSDKP
                     QTTRNTLRGILTGPDYQIIWLDTPGLHRPQHELGNRMITAAQSALTEADLILWVLDAA
                     AGLTAADLKVATTIREAGPPVLAVWNKMDQLAAGTSTPELPGFPKIYHVSAVSGAGLD
                     QLLAVIITALPEGPQYYSPELVTDHPERFIIAELIREQVLNMTEDEVPHSVAVQVEAV
                     KERAAGPTVVEAVIYVER"
     gene            complement(703..1191)
                     /locus_tag="VF531_03570"
     CDS             complement(703..1191)
                     /locus_tag="VF531_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713077.1"
                     /translation="MDPETNNITVEIVTKVDDYLREIMLTIDEEAFGPGSLNEWSLPV
                     FLYYGRVYVARLNAKPVGIAELMRDWRDPELVYLYGYAVARDYRGSGIGTALLRNILE
                     ALPRAGFRRLQLTVHPENQIAIHLYEDKFRMRRVEFVKNYYGSGEDRWLLEWKMDELE
                     YE"
     gene            complement(1224..1655)
                     /locus_tag="VF531_03575"
     CDS             complement(1224..1655)
                     /locus_tag="VF531_03575"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX7713078.1"
                     /translation="METAIHERLIAAATEVRERAYTPYSHYKVGAALLGKSGRIYTGC
                     NVENVSYGATICAERSAVVKAVSEGETTFTALAVIADGDLPASPCGICRQFLSEFGGD
                     LRLILANLRGERKEITLRELLPQAFDSQYLEQSANQATDER"
     gene            complement(1734..2432)
                     /locus_tag="VF531_03580"
     CDS             complement(1734..2432)
                     /locus_tag="VF531_03580"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX7713079.1"
                     /translation="MRARSLLDSFNYAFDGILYVFKTQRNMRLHFLAAAVILLLSLFF
                     KLDKAELLILFLVIALVIVTEMINTAIETVVDLVTQELHPMAAIAKNVAAGAVLVAAV
                     VAIVVGYLIFFPKLDPFIPLVITKLQQTPAYLSLVALVLTVGLVIVGKSLTKSGRPIQ
                     GGMPSGHTALGTAAATTIACITRNSLIVLLLILMIFLIAESRIETRVHSLPEVLAGGL
                     VGFLATVIIFQLMR"
     gene            complement(2508..3038)
                     /gene="ybeY"
                     /locus_tag="VF531_03585"
     CDS             complement(2508..3038)
                     /gene="ybeY"
                     /locus_tag="VF531_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7713080.1"
                     /translation="MGIFLNNLQKAVEILPEQEHLLERVIESGLTLHHHENAEVSMIL
                     VDNDYIQNLNQQYRGLDQPTDVLSFAMLEESAEEIPIAPAEPPIAEKESPIKQESPAE
                     SENQPELLGDIFISMERAREQAADYGHSVNRELCYLAVHGLLHLIGFDHQNPMDQERM
                     RRAEEEILRAAGLGRE"
     gene            complement(3019..>4473)
                     /locus_tag="VF531_03590"
     CDS             complement(3019..>4473)
                     /locus_tag="VF531_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX7713081.1"
                     /translation="RINRLENRVRMEVPEVVHHQGELLIAKNQVITENDMKMLEELHL
                     IVDKTNQVKVFLSLAVFILILIGVGWVYIYQFHSELFKQERLLYLLILILLLAIGMIK
                     VLSLVDNQAIAYLAPVSFAAMLITILIDSQLALAMTIMLSLLGGIIVEYNLALTIFYF
                     VSGVVAVLTLSGFHRQRDLVRSGSILMVSNAVLVTVLNLLFQTSFNYGAVLLATAGGF
                     LSAVLAIGSLPFLENIFKITSAIRLLELSNPGHPLLRRLQIEAPGTYQHSVMVGNLAE
                     AAAEGIGADALWARVGSYYHDIGKIRRPYFFVENQFSQENPHEKLNPTLSTLIITYHV
                     KEGAEIAREHGLPDKLIDIIEQHHGTDLVRYFYKRAAENAQTELPEEDFRYEGPKPQS
                     KEAALVMLADSVEAAVKSLPKPTPSKVETVVQKIIRERLDDGQFDECNLTLKDLTKIK
                     NSFLKVLGGLFHNRIEYPETVLKEIEKKNGDIPK"
BASE COUNT         1091 a         1260 c         1103 g         1019 t
ORIGIN      
        1 tcgcgttcca catagatgac cgcctctacc acggttggac cagccgcgcg ctccttcacc
       61 gcctctacct gaacggcgac ggaatgcgga acctcatcct cggtcatatt cagcacctgt
      121 tcccggatga gttcggcaat gataaaccgc tccgggtgat cagtcacaag ttccggtgaa
      181 tagtattgcg gcccctccgg taaagcggtt atgatcaccg ccaataattg gtcaagcccg
      241 gctccggaaa cggcggaaac atgataaatc ttcgggaaac ccggcaattc cggtgtggaa
      301 gtcccggcgg cgagctggtc catcttgttc cagaccgcca acaccggggg accggcctcc
      361 cggatcgtgg tggcaacctt aagatccgcg gcagttaaac ccgcagccgc atccaaaacc
      421 cagagaatca ggtccgcctc ggtcaacgcc gactgggcgg cggtgatcat tctgttgcct
      481 aactcatgtt ggggccggtg cagtccggga gtatccaacc agatgatctg gtagtcgggc
      541 ccggtcagga tcccccgcag cgtattccgg gttgtttgag gcttatcgga ggtgatcatc
      601 agtttttggc cgagaaaggc attgagcatg cttgacttgc ctacattggg gcgtcccgcg
      661 aggaccacaa aaccggattt aaaggtcttc ttatctttcg ttttactcat attcaagctc
      721 gtccattttc cactcgagca accagcgatc ttcgccggag ccgtaataat ttttaacgaa
      781 ctccaccctc cgcatcctga atttatcctc gtatagatgg atggcgatct gattctcggg
      841 atgcaccgtc agttgtaacc gccggaatcc ggcccggggt aaagcttcaa gaatattgcg
      901 aagcagggcg gtgccgatac cgctgccgcg ataatcacgg gccaccgcgt atccatatag
      961 ataaaccaat tccggatcgc gccagtccct catcaattcg gcaatcccca ccggtttggc
     1021 gttaagacgg gcgacataaa cccggccata atacaaaaat accggcaagg accattcgtt
     1081 caggcttccg ggcccgaatg cctcctcgtc gatggtgagc atgatctcgc gtaaataatc
     1141 gtcaaccttg gtgacgatct ccaccgtgat attgtttgtt tccggatcca tatttcctcc
     1201 tgaaaatcaa tgcctgttcc cgactatcgt tcatccgttg cctggtttgc gctctgctcc
     1261 aggtactggg aatcgaatgc ttgagggagt aactcccgta gagtgatctc cttccgttca
     1321 ccacggaggt tggccagtat caagcggaga tcgccgccga actccgacag gaactgacgg
     1381 caaattccac acggagaggc cggcagatcg ccatcggcga tcaccgccag agctgtaaag
     1441 gtagtctctc cctcggagac cgcttttacg accgcacttc gctcggcgca aatggtagcc
     1501 ccgtaagaaa cattctccac attgcagccg gtatatatcc tacccgactt ccccaaaagc
     1561 gcggcgccaa ccttataatg ggagtacggc gtgtaggccc gttcccggac ttcggtggct
     1621 gccgcgatca gacgctcatg tatggctgtt tccaagcgaa tcccccttaa aatcgttatt
     1681 cgttcccggt tggcggtagt gacttcgaac ctttataata tgaaacaacc ctgctatcgc
     1741 atcagctgaa agatgattac tgtggcgaga aaacccacca accctcctgc caaaacctcc
     1801 ggtaatgaat ggacccgggt ttcgatccgg ctttcggcga tgagaaagat cattaatatc
     1861 aaaagcagga caatcagact gttacgggtg atacaggcga tagtggtggc cgccgcggtt
     1921 cccagcgccg tatggccgct tggcattcct ccctgaatcg ggcggccgct cttggtgagt
     1981 gatttaccca cgatcactaa acccacggta agtaccaagg ccaccagact cagataagcc
     2041 ggggtttgtt gcagtttggt aatgaccagc ggaatgaagg gatcaagttt cggaaagaag
     2101 attaaataac ccacaacgat cgcgaccacg gcagccacta aaacagcgcc cgccgccaca
     2161 ttcttggcga tcgccgccat ggggtgcagt tcctgagtga ccaagtccac tacggtttcg
     2221 atggcggtgt tgatcatctc agtcacgata accagcgcaa tcactaaaaa gaggatcaat
     2281 agttccgcct tatccagctt aaaaaagagc gataaaagca gaatgaccgc agccgccaag
     2341 aaatgaagcc gcatattccg ctgagtttta aagacgtata aaataccatc aaaggcgtaa
     2401 ttaaatgaat caagcaatga acgtgcccgc aaatccgtat cctccagtca tcacaaagct
     2461 gcggcacggt atcggccggt cgatcttccc ggatccgcgg ccggagttca ctcccggccc
     2521 aatccggcgg ctcgcaagat ctcttcctca gcccggcgca tcctctcttg atccatcggg
     2581 ttctgatggt cgaagcctat caaatgaagc aacccgtgaa ccgccagata acagagctcg
     2641 cgatttacgg aatggccata atcggccgct tgttccctag cccgttccat ggagatgaag
     2701 atatctccca agagttcggg ttgattctcc gactctgcag gagactcctg ttttatcgga
     2761 gactctttct ccgcgatcgg gggctctgca ggcgcgatcg ggatctcctc ggccgattcc
     2821 tcaagcatcg caaaggaaag tacatcggtg ggttggtcca gaccacgata ttgctgattc
     2881 aaattttgaa tataatcatt gtccaccagg atcatgctga cctcggcatt ttcgtgatga
     2941 tgcagcgtca ggccggactc tataacccgc tctagaagat gctcctgttc cggcaggatc
     3001 tccacggctt tctgaagatt atttaggaat atccccattt ttcttctcga tctcctttag
     3061 taccgtctcc ggatactcga tccggttatg gaaaaggccg cccaacacct ttaaaaaact
     3121 atttttgatc ttggtcagat ccttcaatgt caggttacac tcatcaaact ggccgtcatc
     3181 cagccgttcg cggatgatct tttgaactac cgtctccacc ttggaagggg ttggcttcgg
     3241 caacgatttg acggccgcct ctaccgaatc ggccagcatc accagggcgg cttcctttga
     3301 ctggggcttg gggccttcat aacggaaatc ctcctcggga agctctgttt gagcattctc
     3361 cgcagcccgt ttataaaagt aacgcaccag atcggtcccg tgatgttgtt cgatgatgtc
     3421 gatgagttta tccggcaaac cgtgctcccg ggcgatctcg gcgccttcct tcacatgata
     3481 ggtgatgatc aaagtgctca aggtgggatt cagtttctcg tgagggttct cctgagaaaa
     3541 ctgattctcc acgaagaaat atggccggcg tatctttccg atatcatggt aataggagcc
     3601 gacccgggcc caaagagcat ccgccccgat tccttccgcc gcggcttccg ccagattacc
     3661 caccatcaca ctatgctggt aggtcccggg agcctcaatc tgcaaccgcc gtaacaaagg
     3721 atggcccggg ttggagagtt caagcaaccg gatggccgag gtgatcttaa agatattctc
     3781 caaaaacggc agtgagccga tggcgagaac cgccgagaga aatccacccg ctgtagccag
     3841 caaaactgcg ccataattga agctggtttg gaatagcaga ttcagtaccg tgaccaaaac
     3901 tgcgttggaa accatcaaaa tcgaaccgct gcgtaccaga tcccgttgac ggtggaatcc
     3961 ggagagggtc aataccgcca ccacgccgct gacgaagtaa aatatggtca acgccagatt
     4021 gtactcgacg atgatccccc cgagcaggct gagcataatc gtcatcgcca gcgccagttg
     4081 ggaatcgatc aaaatggtaa tgagcatcgc cgcgaaactc accggagcca gatatgcaat
     4141 ggcctgattg tcaaccagcg ataagacctt aatcataccg atggccaaga gcagaattag
     4201 gattaacaga tataataacc gttcctgttt aaagagttcg gaatgaaact gatagatata
     4261 gacccagccc accccgatta agatcaggat aaatactgcc agactcaaaa agaccttcac
     4321 ctgattggtc ttatccacaa ttaagtgcaa ctcttccagc atcttcatat cattctcggt
     4381 gatgacctgg tttttggcga tcaagagttc gccctgatga tgcacaacct ccggaacttc
     4441 catccggacc cggttttcca agcggttgat ccg
//
LOCUS       DASVMB010000111         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_14037,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000111 DASVMB010000000
VERSION     DASVMB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14037"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /gene="tsf"
                     /locus_tag="VF531_03595"
     CDS             <1..629
                     /gene="tsf"
                     /locus_tag="VF531_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7713082.1"
                     /translation="FVAIVNNLARQAARTSASTNLELMAERYDADPAITVQDAVTGLI
                     AKMRENISVRRFQSFQTPAGFVHGYLHHGARVGVLLEFSCKKESPVLASIGKEVAMQI
                     AATNPIFIDRSAVPPEVLDDQRRAFRTNAQKAGKPEALLDKIAAGKIEKYLKEKCLME
                     QAWIKNEDMTIGDYLREESRKLGQTVGIGRFTRFERAENLDGDTHCQE"
     gene            797..>1780
                     /locus_tag="VF531_03600"
     CDS             797..>1780
                     /locus_tag="VF531_03600"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX7713083.1"
                     /translation="MLWEHLAFIIILFITSSIAGILAFFTYRRRNRPGAKEFTALLIS
                     ITIYSFGYACELTSYTLPQVIFWLRVEYLGISFIPCFLILMVSSYTGLKIRNNPWLVV
                     VLFSISLTILIFHCTNEYHHLYYRSIHLAKYGNFSVTMFEKGPWYWVSVIYAYLSLVF
                     CNIVLFQMRMQAGSLFRKQINIIILSLQVPGVASLFYLFGLNPLHLDLIPFSFTLTGF
                     IIWRGLFFHRLFDLSPIAREKLFENIRDGVIVLDLENRIVDYNQKAKNILMELNPVDI
                     GQKAVNVCHDYPELAKQLFDNLERNEFYVTTGTRKKCMESRLSLILSDRKEL"
BASE COUNT          471 a          347 c          437 g          525 t
ORIGIN      
        1 attttgtagc gatagttaac aacctggcca gacaggccgc gcgaacatct gcttcgacca
       61 atcttgagtt gatggcggaa aggtatgacg cggatccggc gattacggtc caagatgcag
      121 tgacagggtt aattgccaag atgcgggaga atatttccgt ccgcagattt caatcttttc
      181 aaactccggc gggatttgtc catggatatc tgcaccacgg cgccagagtc ggggtattgc
      241 ttgagttttc ctgtaaaaag gagagcccgg ttttggcgag tatcggcaag gaagtcgcca
      301 tgcagatcgc ggcgacgaac cccatcttca tcgatagaag cgccgttccc ccggaagtct
      361 tggatgacca aaggcgggcc tttcgcacca atgctcaaaa agccggaaaa ccggaggctc
      421 tgttggacaa gatcgcagcg gggaagattg aaaaatacct caaggaaaag tgtttgatgg
      481 agcaagcatg gattaagaac gaggatatga caattgggga ttatttacgt gaagaatcgc
      541 gaaaactggg tcagaccgtc gggatcggcc ggtttactag gtttgaaaga gccgagaatt
      601 tagatgggga tacgcactgt caggaataga taaaaaagag ggggggcaaa cccctctttt
      661 ttttgcccgc aaaaccacat gattacccat ttagacttca aatcattcaa aaaagagtta
      721 aggaaacgca gcttagaggc cgaatcttta gttgaagata ttaagcggaa aataagtatc
      781 tttgggggca actgaattgc tctgggaaca tctcgcattt atcattatct tgtttattac
      841 cagtagtatt gctggaatat tggcattttt tacataccgg cgtcgtaacc ggccgggggc
      901 taaagagttc accgctttat tgatttcgat aacaatttat tcgttcgggt atgcttgtga
      961 gcttaccagt tataccttac cccaggtgat attctggttg agagttgagt atttgggtat
     1021 ctcattcata ccgtgtttcc tgattctcat ggtcagcagt tataccgggt taaaaatccg
     1081 gaacaatcct tggttggttg ttgttttatt ctctatctcc ctgactattc tcatctttca
     1141 ttgcaccaat gaatatcatc acctgtatta tcgttcaatc catttagcta agtacggtaa
     1201 tttctcggtg accatgtttg aaaaaggacc ctggtactgg gtaagtgtga tctacgcata
     1261 cttgtcactg gtcttttgca acattgtgct ttttcagatg cgaatgcagg cgggatcgtt
     1321 atttcgcaaa cagattaata ttatcatttt gagtttgcag gttcctgggg tggcgtcgct
     1381 gttttatctt ttcggattaa atcctcttca tttggatttg attcctttta gttttacgtt
     1441 aacgggtttc attatctggc gtgggttgtt ctttcatagg ttgtttgatc tttcgccgat
     1501 tgcccgggag aaactttttg aaaacattcg ggacggcgta attgtcctgg atctggaaaa
     1561 ccggatcgtt gattataatc agaaggctaa gaacatttta atggaattaa atccggttga
     1621 catagggcaa aaggccgtga atgtatgcca tgattatccg gaattggcga aacagctttt
     1681 cgacaacttg gaacggaatg agttttatgt caccaccggg acaaggaaaa aatgtatgga
     1741 atcccggcta tctctcattt tatctgatag gaaagaatta
//
LOCUS       DASVMB010000112         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_160651,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000112 DASVMB010000000
VERSION     DASVMB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160651"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..822
                     /locus_tag="VF531_03605"
     CDS             67..822
                     /locus_tag="VF531_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7713084.1"
                     /translation="MMEYLEKHESMTVGDICGIFQVSRDTARRDIVKLTEQGAVIRTH
                     GGIALTKFNQKIQEYVSRLTNEPEVKKEIGAYAATYVKDNDMIFLDVSTTVQCMIPHL
                     KVQSLTVITQSMDNAWLLMENDRVQTYIMGGLIQPKTRQALGYDTLAKLADYRFNKIF
                     IGATGITHEGVYYFAEEDISFKRELAKRAEQVIILADHTKFGKYSSYKGLDLNAVDIM
                     ITDRPVAKGLQENIESEGIQLITLQDIEEDGNV"
     gene            815..1447
                     /locus_tag="VF531_03610"
     CDS             815..1447
                     /locus_tag="VF531_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7713085.1"
                     /translation="MYEAVVFDVDGTLIDTEKAILCSLQRTLKEEMNREYPLAELTFV
                     LGIPGTASLQQLGVRNVDGLLHKWDLYMRDFCDEIHVFTGIEPVLNELRRLGIKTGIV
                     TSKTRQELIADLSPFQLARFFSYTVCADDTRKHKPEPEPLLKLMEVADIRPEKAVYIG
                     DTLYDLQTAQGAGVDFGLALWGANNKVMPGAKYRFGSPNEILSIITGDRK"
     gene            complement(1607..2326)
                     /locus_tag="VF531_03615"
     CDS             complement(1607..2326)
                     /locus_tag="VF531_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713086.1"
                     /translation="MNIIKRAVLEDFKLVSREPMVAVLFGVPLFVSMFMRFIVPLGTD
                     LLFKYTGFNLSAYNLYVLASILLMVPTLVGPSAGFLMLDEKDDNVQVLSLVTPGGISG
                     YLTARLLMPAVAGFVYTILSYLITGFYPITPDALFICALLAAAQGILFSFVLFAFADD
                     KVKGLTFVKGMSLFEVLALADLMNNHWISAVAACTPLYWTVKVMEFSGTWRVWLSGLL
                     GLVVNGVWLAVTWRKARKKIY"
     gene            complement(2323..3030)
                     /locus_tag="VF531_03620"
     CDS             complement(2323..3030)
                     /locus_tag="VF531_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713087.1"
                     /translation="MNRVYQALKADLSFQMKQGFYAIYLVISVMYIVVLYCFPMDLRK
                     IIAPLCAYSDPAIVGMLFTGAQLMLEKNQGVLSYIAVTPLAPREYIGAKVLSFGIFGS
                     VMALLLAIATIGFNFNLFYYLAGIVLTSAMFTSIGVMVGVKSRSMNELIVKLIPLMII
                     MTLPCLGIIGFKYSPILEIFPSLPALEVVFAAFNGLAPLKVIMYLLYVLLWDIFLFKK
                     AVASFDGYLVMKGGNAG"
     gene            complement(3027..3881)
                     /locus_tag="VF531_03625"
     CDS             complement(3027..3881)
                     /locus_tag="VF531_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713088.1"
                     /translation="MIQVQRLGFIYPKAVKPALSAIEFQVKEGEIFGFLGPSGAGKTT
                     TQKIIIGLLGNYSGSVKIFGKERCEMGRDFFEHIGVAFEMPNLYQKLTAYENLKLFAS
                     YYQNRPRSFDELLDQVGLLADRNQKVENFSKGMKMRLNLIRSLMHQPEVLFLDEPTTG
                     LDPVNSKKVMDIVRDLKRAGRTVLITTHNMNVADELCDRVAFIVDGRIRLIDSPGELK
                     LKYGKRRVDVEYREAGEERKASFHLDGLGDNPDFIDLIQNRYILRIHSQEATLDEIFI
                     KVTGRELI"
     gene            complement(3934..>4188)
                     /locus_tag="VF531_03630"
     CDS             complement(3934..>4188)
                     /locus_tag="VF531_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713089.1"
                     /translation="TVKEQLVAMAAAEFQKHYQEQGIQWVNPEFGTILARQFIEGIVL
                     ILTEYPTEDKRQTMMAEHFLFYIVGVMALVMGINWPNLKK"
BASE COUNT         1192 a         1016 c          957 g         1023 t
ORIGIN      
        1 acttaaaaat acttataata aggtttgagg tgaatcatta tttaccagga agagcgttta
       61 tatcggataa tggagtattt ggaaaagcac gagtccatga ctgtcggcga tatttgcggt
      121 atttttcagg tctccagaga cacggcacga cgggacattg tcaaactgac agagcagggt
      181 gcggtgattc gaacccacgg cggtattgcc ctgaccaaat tcaatcaaaa aattcaggaa
      241 tacgtatcgc ggttaaccaa tgaacccgag gtaaaaaagg agatcggggc gtatgccgct
      301 acctatgtca aagataacga tatgatcttt ctggatgttt cgaccacggt tcaatgcatg
      361 atccctcacc tcaaggtgca gtccctcacc gttattacac aatcaatgga taatgcctgg
      421 ttgttgatgg agaatgatcg tgttcaaacc tatataatgg gtggtctgat ccaacccaag
      481 acgcgacaag cgctgggtta cgatacattg gcgaagctgg ccgattaccg ctttaacaaa
      541 atcttcatcg gcgccacggg catcactcat gagggtgttt attattttgc cgaggaggat
      601 atcagtttca aacgtgagct ggcgaaaagg gcggaacagg tcattatact cgcggatcac
      661 accaaatttg gcaaatactc ttcttataaa gggctggatt taaatgcggt cgatatcatg
      721 attaccgatc gccctgttgc caaagggtta caggagaata tagaaagcga gggcattcag
      781 ttaatcacct tgcaggacat agaggaagat ggaaatgtat gaagcggtgg ttttcgatgt
      841 ggacggcacg ctgattgata ccgaaaaggc catcctctgc tcactccaaa ggactctcaa
      901 agaagaaatg aatcgtgaat accctttggc ggagttaacc tttgtactag gaattcccgg
      961 aacggcttcc ttgcagcaat taggagtccg gaatgtcgat ggtttgcttc acaaatggga
     1021 tttgtatatg agagactttt gtgatgagat tcatgtcttt accggcattg agccggtact
     1081 gaacgaatta aggcgcttgg gtatcaagac cggaattgtt acttccaaaa ccagacagga
     1141 attaatagca gacttgagtc cgtttcaatt ggcacggttt ttttcgtata cggtttgcgc
     1201 cgatgatacc agaaaacata aacccgaacc ggagccgctc ttgaagctga tggaagttgc
     1261 cgacatccgg cccgaaaagg ccgtttatat tggagatacc ctttatgatc tgcaaacggc
     1321 tcaaggtgcg ggggtcgatt ttggattggc gttatgggga gccaataata aggtgatgcc
     1381 tggtgccaaa tatcgatttg gcagcccgaa tgagatactt tctataataa ccggtgatag
     1441 gaaataaaaa tttctctcgt catttattcc ttttttcggc ggcatggatc tcccctaatt
     1501 gattttggtg tggaggcgcc gaagccggcg taaccgcaaa gcgggcgtaa cggctttggc
     1561 gacctccctt ttaaattttt gataaacaac gaatccatat caacggctaa taaatcttct
     1621 tccttgcctt acgccaagtg acggcaagcc agaccccgtt caccaccaat cccagcaaac
     1681 cggataacca gacccgccag gttccggaaa attccattac ttttacagtc cagtataggg
     1741 gtgtgcaagc agcgactgcc gatatccaat gattattcat cagatccgcc aaggccaaga
     1801 cttcaaaaag gctcatccct ttgacaaacg ttaagccctt taccttatcg tcggcgaagg
     1861 cgaacaggac aaatgaaaac aagatcccct gagccgcggc gagcagtgcg cagatgaaca
     1921 gggcgtccgg ggtaattgga taaaaaccgg tgatcaagta actgagaatc gtataaacga
     1981 acccggctac ggccggcatc aacaacctgg cggtaagata gccgcttatg cctccgggag
     2041 taactaagga caggacttgc acattatcat ccttctcgtc cagcatcaaa aagccggcac
     2101 tgggtccgac gagggtaggc accattaaca agattgaagc taaaacatag agattatagg
     2161 cagaaagatt aaatccggta tacttaaaca gtaagtcggt gcccaaaggc acgataaacc
     2221 gcatgaacat gcttacaaat aacgggaccc caaacaacac ggcaaccatc ggctccctgg
     2281 atacgagctt aaaatcctcc aaaacggccc gcttaatgat attcaaccgg cattcccccc
     2341 tttcataacc agataaccgt cgaatgatgc gaccgccttt ttgaagagaa atatatccca
     2401 aagtaaaaca tagagcagat acatgataac cttaagagga gcgagaccgt taaaagcggc
     2461 gaaaacgacc tcgagagccg ggagggacgg aaagatctca aggatggggc tatatttaaa
     2521 cccgatgatt cccaaacacg gtaatgtcat aataatcatc agaggtatca gtttaacgat
     2581 aagttcattc atactccggc ttttgacccc aaccatcacc ccgatgctgg taaacatggc
     2641 ggaagtgagc acaattcccg ccagataata aaatagatta aagttaaacc cgatggtggc
     2701 gatagcaagc aacaacgcca tcaccgaacc gaagatgccg aatgaaagca ccttggcccc
     2761 gatatactcc cgcggtgcca ggggtgtaac tgcaatataa ctcaagactc cctggttctt
     2821 ctccagcatt aactgtgccc cagtgaaaag cattcccact atcgccggat ctgaataggc
     2881 gcacagcggt gcgatgattt tacggagatc catcggaaaa caatacagta ccacgatgta
     2941 catcaccgat ataaccaagt aaatcgcgta aaacccctgt ttcatttgaa aactcagatc
     3001 cgcttttaag gcctggtaaa cccggttcat atgagctccc tcccggtaac cttgatgaag
     3061 atttcgtcaa gagtcgcttc ctggctatgg atccgcagga tataccggtt ctgaatcaga
     3121 tcgataaaat cgggattatc tcccaaacca tctagatgaa aagaggcttt tcgctcttct
     3181 ccggcctcgc gatattccac atcaacgcgt ctcttgccat acttcaactt gagctctccg
     3241 gggctgtcaa tcaaacgaat ccgcccgtca acgatgaaag ctaccctatc gcaaagctca
     3301 tcggcgacat tcatattgtg cgtggtaatt aagacggttc tcccggcccg tttcaaatcc
     3361 cgcacgatat ccatgacttt tttggaattg acgggatcca aacctgtcgt gggctcatcc
     3421 aggaaaagaa cttccggttg gtgcatcagg gaacggatta agttaagccg catcttcatc
     3481 cctttggaaa aattctcgac tttttgattt ctatccgcca gcagtccgac ctgatccaaa
     3541 agttcatcga acgaccgggg ccggttttgg taataggatg cgaataactt caggttttcg
     3601 taggctgtca gcttttgata gagattgggc atctcaaatg cgaccccgat gtgttcaaaa
     3661 aagtcccgcc ccatctcgca gcgctctttg ccgaagatct tgacgctccc ggaataatta
     3721 ccgagtaggc cgataattat cttctgggtg gtggtttttc cggcaccgct cggtccgaga
     3781 aaaccgaaga tctcgccctc cttcacctgg aactctatag cgcttagcgc cggcttcacc
     3841 gccttgggat aaataaaccc caacctctgc acctgaatca ttgatagacc tcctttaata
     3901 aataacagcc gttctttttt aattgaaaaa aatttatttc ttcaaatttg gccagttaat
     3961 gcccatcacc agggccatca ctccgacgat gtaaaagaga aaatgctcgg ccatcatggt
     4021 ttgtcgtttg tcctctgttg ggtactcggt caagatcaag acaatccctt caataaactg
     4081 ccgggccaag atagtgccga attccggatt cacccactga atcccttgtt cctgatagtg
     4141 tttttgaaat tcggccgcgg ccatcgccac cagctgttcc ttgacagt
//
LOCUS       DASVMB010000113         7750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_602571,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000113 DASVMB010000000
VERSION     DASVMB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602571"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..707)
                     /locus_tag="VF531_03635"
     CDS             complement(441..707)
                     /locus_tag="VF531_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HEX7713090.1"
                     /translation="MAYQFTFTRRFQKHFKSLTEQEKKQLRNKLKLLVENPMHPSLRT
                     KRIQGVEDLFECSVNTDIRIIWYYEGDTMIILVDVGHHDILKQF"
     gene            complement(707..967)
                     /locus_tag="VF531_03640"
     CDS             complement(707..967)
                     /locus_tag="VF531_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7713091.1"
                     /translation="MLAEFRAKSQITIPKELVDKLGLSEGDKLEVYEENGIICIMPVA
                     VYPKKYLNELRKEIEETKAKIASGEQPVFDNVDALFAKLEGN"
     gene            complement(1289..4510)
                     /locus_tag="VF531_03645"
     CDS             complement(1289..4510)
                     /locus_tag="VF531_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7713092.1"
                     /translation="MGKRKFIPIILAVVLLSVLCAFGTVWAEAGVKLTILGTSDLHGR
                     LYPWEYAIDSEDPASGLAKVASVVKTVRAENPNTVLVDDGDLIQDNMAELFNNEKVHP
                     MVQAMNEIGYQTWTLGNHEFNYGLDVLGRAIRGSKATVLAGNIYQANGKRFVNAYKIV
                     KMNGVRVAIIGMITPHVPRWEASDPAHFKGLTFTDPAVETKKVISELRGKADILIGVM
                     HMGLDPEYGVDGSGIRAVAAANPELTAIVAGHAHSDIMGETINGVLVVEPRSNGNKVS
                     RIDLLIAKENNQWKVESKTSQNIDVSKFPADPAILAKFKPYHDRAIAEANTVIGKITA
                     GFLPERELLPGIPTAQVQDTALIDFINEVQMAYTKADVSSAALFSSGANLAQGDFKKK
                     DVANIYKYTNTLMAVKVTGKQLKAYMEWSAQFYNQAKPGDLTVSFNQNIPGYNYDMFS
                     GVSYELNVAAPAGKRIENLTFKGKPVTDDQTFTLAVNNYRYGGMVKDGYFKASDLVGE
                     YGQIRDLIVDYVQKNRTISPKVDNNWRLTGYSPDHPLRNEVFQKVKAGEIKIPASANG
                     RTQNVKALNVYELCDSGALNYRIIDLLSITDFHGSLTKSGKNVGIANVVGEIKKWRAV
                     NPNTIFVSGGDLYQGSAESNLLYGKPVVEALKEAGLVASAIGNHEYDWGAGRIAGWAK
                     DGGFTFLAANIVDKQTQQPASYTKPYLIVDCGGVKVALIGFTTPETAYKTNPLNVADL
                     EFKEPAAVLPEYIKEVRNQGADLVIALTHLGGVQDKSGQITGEAADLAKVPGLDGIIA
                     GHSHNPIAGVVGKTPLVMAYYNGRTIGRLSLIVTKDKHQLVGSQAVLDNLYLRPATLT
                     EDSAAKAIYDKYMAQVNPILAEKLGEALVDLNHDTQGPSLMGEWVCDIMRQKTGAQIG
                     IQNGGGLRVSLPKGDLTVGSMYTLMPFDNTLVTAKLTGAQVREAIENGLGNDKISFGQ
                     VSGVFVTYDLTKPFGQRIISVTLENGEPLDPNKLYTVVANDFMFSGGDNYTSLQKGQE
                     IKDTGIPVRDAIADYVKQNKTIKPAYRGCQKPVTEAAKQQPAA"
     gene            complement(4826..>7750)
                     /gene="ileS"
                     /locus_tag="VF531_03650"
     CDS             complement(4826..>7750)
                     /gene="ileS"
                     /locus_tag="VF531_03650"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7713093.1"
                     /translation="YHTMLGYQVNRKAGWDTHGLPVEIEVEKSLGLSNKTEIEKYGIE
                     AFNKKCRESVFTYEREWRRMTERIGYWLDMDHPYITLDNSYIESEWWILKRYFDEGLI
                     YEGHKIMPYCPRCGTPLASHEVSQGYKDETIDSIYIRMQVSGKPGEYFLVWTTTPWTL
                     PSNVALAVNPSYTYVRARRPEDPAVYILVRDRVDAVLGKGAEILEEFPGSKLVGESYE
                     QFLPFIKVNEATAFKVYGAEFVSTEDGSGIVHIAPAFGEDDYQLGRKYQLPVLQPVDK
                     EGKFTPEITPWAGRFVREADSDIIRQLKVDGKLFHKERMTHSYPYCWRCDTPLLYYAR
                     KSWYIATTKYKERLIANNQKIKWYPEHVGKGRFGNWLENLIDWSLSRDRYWGTPLNIW
                     RCESCGKLTSVGSRQELAERAIETVDPATIELHRPYIDEVHLKCGCGGRMTRTKEVID
                     CWFDSGSMPYAQWHYPFEHKDDFDKLFPADFICEGIDQTRGWFYSLLAIGTFLFDLPT
                     YKRVLVNDLLLDKNGQKMSKSRGNAVDPWKLLDKYGADATRWYLLAVSPPWTPTRFDE
                     DGLKDTYAKFFGTLQNVFSFFTLYANIDNADPENYNVPVADRDEIDRWIISRMNSLIR
                     QARTDIEAYDLTRVVREIQAFVIDEVSNWYVRRSRDRFWASEMDTHKKAVYRTLWEVL
                     LNVAKLMAPFAPFMAEEIYQNLKQGPDKLTSVHLEMYPESDESLINVDLEEHMGLVIN
                     LVSLGRAARNKVQIKVRQPLATILVARKHQPILAPMEGLVKEELNIKNLEYIDNPDEY
                     VAYTVKPNFPVLGPKYGKLMKDIATALAAGNAAEMVKTLRETGALQLTVAGNQVTLGN
                     EDLEVRVEEKQGFAVEMDHGDYVILNTVLTQSLVQEGLARELVSKVQNMRKTANFELT
                     DRIRLDFTVDDEVAAAVEAFKDYVMAETLAVAVNRETSKAAGFEEWDLNGHPAAIKVA
                     KE"
BASE COUNT         1836 a         2071 c         1833 g         2010 t
ORIGIN      
        1 acatacacga ccaaacccac ccaaataaag gctgatccac tttttacgat ctccctaccg
       61 gtttcatcta gaattctttt gataaagttg acttcctgaa tggcctcttc tttattcatc
      121 tgagtcctcc tttttattgg catgttattc aatttatgaa gtacttcgta aaatgaagta
      181 taataccaaa atagttattt gtcaatatta gtaattatta tataaattta gtaagccaat
      241 attttcaaag tcaagaatta atcttcttcg cctcaaaaat cagggctact cttccagatt
      301 ccaagcctag agttactctt cacgtaaatt gcccccaaag cctaagggtt gagagttagg
      361 tgggagagag tgcgcgtcca aaggatgcga aaacaagtag ggtgagtcgt ttttggggca
      421 tcacaaaaac acagaatatg ctaaaactgc tttaaaatat catgatgccc tacgtcaaca
      481 aggataatca tagtgtcacc ttcatagtac cagatgatac gaatatccgt gttgacactg
      541 cactcgaata aatcctctac gccttgaatg cgctttgtcc gcaaagaagg atgcatcgga
      601 ttttccacca acaatttcag cttgttccta agctgtttct tttcctgctc ggtcagcgat
      661 ttgaagtgct tctggaaacg gcgggtaaag gtaaattgat acgccatcag ttcccctcca
      721 gctttgcgaa gagcgcgtcc acattatcaa aaacgggctg ttcaccggag gcaattttcg
      781 cttttgtttc ttcaatttct ttccggagct cattcaaata ttttttggga tacacggcta
      841 ccggcataat gcagattatg ccattttctt cataaacttc caatttgtca ccttcggaaa
      901 ggcccagttt atccacaagc tctttgggta tggtaatttg tgatttggcc cgaaactcag
      961 ctaacatggc ttcatcatct cctattatct gaagttgtaa tttcttactt tcctactaat
     1021 agtatatgat gtttttaaaa gatatcaacc ataaaaactg caactgcatc gggccaactt
     1081 agtccgaggt ttttgtcgtg cgatctatat ctatttatat tcagcttgcc gcctttgatc
     1141 cccttcttcc ctctttcccg caaagcggta gagtgtgcat ccaaaggacg cgagagcaag
     1201 caatgtgagt cgcttttgta acattataaa aaaccgacgc cccggaatgc ggggcgccgt
     1261 aatgaataat ggtctattga gctagatctt aagccgccgg ctgttgcttg gccgcttcgg
     1321 tcaccggttt ctgacacccc cggtaggcag gtttgatggt cttattctgt ttgacataat
     1381 cggcgatggc atcccggacc ggaatgccgg tgtccttgat ctcctggcct ttttgaaggg
     1441 aagtatagtt gtcgccgccg gaaaacatga agtcgttggc cactacggtg tacaatttat
     1501 tgggatccaa aggctcgccg ttttccaagg taaccgagat gattctttgg ccgaagggtt
     1561 tggtcaggtc gtaagtgaca aatacgccgg aaacctgtcc gaagctgatc ttgtcattgc
     1621 caagtccatt ttcgatggcc tcgcggacct gggctccggt taatttcgcc gtaaccagcg
     1681 tattgtcaaa aggcatcagc gtatacatgc tgccgacggt taagtcacct ttgggcaacg
     1741 aaacccgtaa accgccgccg ttttggatcc cgatctgggc tccggttttt tgacgcatga
     1801 tatcgcagac ccattcaccc atgagcgatg gtccctgggt atcatgattc aaatccacca
     1861 aggcctctcc cagcttctcc gcaagaatcg gattcacctg agccatatac ttgtcataaa
     1921 tggccttggc cgccgagtcc tcggttaaag tggcaggcct caggtaaaga ttatccagta
     1981 ccgcctggct tcccaccaac tgatgtttgt ctttggtgac aatcaaggag agccggccga
     2041 tggttcgtcc attataatag gccatgacca acggggtttt gccgacaacc ccggcaatgg
     2101 ggttatggga atgtccggcg ataatcccgt ccaaacccgg tactttagcc aggtcggccg
     2161 cctcgccggt gatctggccg gacttgtcct gcacaccgcc aaggtgggtg agtgcgatga
     2221 ccagatctgc cccttggttg cggacctctt tgatatactc cggcaaaacc gccgccggtt
     2281 ctttaaattc cagatccgca acattgagag gattggtctt gtaggcagtc tccggagtgg
     2341 taaaaccaat caaagctact ttgactccgc cgcaatcgac gatcaaatat ggtttggtgt
     2401 aacttgccgg ttgttgggtt tgtttatcaa cgatattggc tgctaagaag gtaaagccgc
     2461 cgtcttttgc ccaaccggca atgcggcccg ctccccagtc atattcatgg ttaccgatgg
     2521 ccgaggcaac cagacccgcc tctttcaacg cttcgactac gggtttgcca tataacagat
     2581 tcgactcggc gctcccctgg tagagatcgc cgccggagac gaagatggtg ttgggattga
     2641 cggcccgcca ttttttgatc tctccgacaa cgtttgcaat gcccacgttc tttccgctct
     2701 tggtgaggga accatggaaa tcggtgatgg agagcaggtc gatgatgcgg taatttaggg
     2761 caccgctgtc acaaagttcg tagacgttca aggccttgac attttgggtt cgcccgttgg
     2821 ccgaagccgg aatcttaatc tcaccggctt taactttttg gaagacttca tttcgtaagg
     2881 gatgatccgg gctataaccg gtcaatctcc aattgttgtc gaccttggga ctgatggttc
     2941 tgttcttttg tacataatcc acaatcaaat cgcgaatctg gccgtattcc ccaaccagat
     3001 cggatgcctt gaagtaaccg tccttgacca tgccgccgta acggtagtta ttcaccgcca
     3061 gggtaaaggt ctggtcatca gtaaccggtt tgcctttaaa ggtcaggttt tcaatgcgtt
     3121 tcccggccgg agcggccaca ttcagctcgt aacttacacc ggagaacatg tcatagttat
     3181 aaccgggaat gttttgattg aagcttacgg tcagatcgcc gggcttcgct tggttataaa
     3241 attgcgccga ccattccatg taagctttga gttgtttacc ggtgactttg accgccatca
     3301 atgtgttggt gtatttatag atgttggcga catccttctt cttgaagtcc ccttgggcta
     3361 gattggcgcc ggaactgaag agggcggccg acgatacatc ggctttggtg tacgccatct
     3421 gaacttcatt gataaaatca attaatgccg tatcttgaac ctgggctgta gggatccccg
     3481 gcaataactc ccgctcgggc aggaagcccg cggtgatctt gcctataacc gtgttggctt
     3541 cggcaatcgc ccggtcgtga tagggtttga acttggctaa gatagccgga tcagccggaa
     3601 atttagaaac atcaatattc tggctggttt tggactcgac cttccattgg ttgttctcct
     3661 tcgcaatgag gaggtcgatc cgggagacct tgttcccgtt ggacctgggt tcaaccacca
     3721 acaccccgtt aatggtctct cccatgatat cggaatgggc atgcccggcc acgatggcgg
     3781 taagctccgg attggccgct gccaccgccc ggatcccgga accatccaca ccgtattcgg
     3841 gatcgagccc catgtgcatc acgccgatca atatgtcggc ttttcctcta agctcggaaa
     3901 taactttctt ggtctcaact gccgggtcgg taaaggtcaa ccctttgaaa tgggcgggat
     3961 cacttgcttc ccagcggggc acatgcggag tgatcatccc gatgatggct acccggacac
     4021 cattcatctt gactatttta taagcgttca caaaccgttt gccattcgct tgataaatat
     4081 tgcccgccaa cactgtagcc ttggagccgc gaatcgcccg tcccagaaca tccagaccat
     4141 aattgaactc gtgattaccc aaggtccagg tctgatagcc gatctcattc atggcttgga
     4201 ccatcggatg aactttttcg ttattaaaga gttcggccat attgtcctgg atcaagtccc
     4261 catcatccac caaaacggtg ttggggttct cggcccggac tgttttgacc accgaagcca
     4321 ccttggccag acccgaagcg ggatcttcac tgtcaatggc atactcccaa gggtaaagcc
     4381 tcccatggag gtcactggtc cccaggatgg tgagcttcac acccgcttcc gcccagacag
     4441 tgccaaaggc gcagaggaca ctaagtaaga ccacagccag aatgattgga ataaacttac
     4501 gtttgcccaa caactttaac cgcacctcct ataatttaaa atcatataca tatatttctt
     4561 ctatatgtaa taaaaacctt tctttcctct ttgaatttaa tatagtccgg ataagaaaac
     4621 cttaaacaaa gttaatggaa atattaaaac caccgaaaaa ataccgctcg gtggtttttt
     4681 taaaacagtc taaaggcgac tcaccccctt catccccctc tctacaacaa ctattgtaga
     4741 gagggggacg gctcaaaagt tacatttagc aaaattaatc tcagggggtg agtcgatgtt
     4801 aggcattcaa aacaaatact ttagctcatt ccttggccac tttgatcgca gccggatggc
     4861 cgttgagatc ccattcctcg aaaccggcag ctttcgaggt ttcacggtta accgccaccg
     4921 ccagcgtctc ggccatgaca taatccttga aggcttccac tgccgcggcc acctcgtcat
     4981 caaccgtaaa atcaagccga atcctgtcgg tcagttcaaa attggcggtt ttccgcatat
     5041 tttgaacctt ggatacgagc tccctggcca ggccttcctg gaccaaagac tgggtcagga
     5101 cggtattcag gatgacatag tcgccgtgat ccatctctac cgcgaagccc tgtttctcct
     5161 ccaccctgac ctccagatct tcgttgccca gggtgacctg attaccggct acagtcaact
     5221 gaagcgcccc ggtttcccgc aaggttttta ccatttccgc ggcattgccg gctgccagcg
     5281 cggtggcaat gtccttcatc agtttgccgt atttcgggcc aagtaccggg aagttgggtt
     5341 tgaccgtata ggccacatac tcgtcgggat tgtcgatata ctccaggttc ttgatgttca
     5401 attcttcctt gaccaggcct tccatgggag ccaggatcgg ttgatgtttc cgcgccacca
     5461 aaattgtggc caggggctgt ctgaccttga tctggacctt gttccgggcg gcccggccta
     5521 aagagaccag attaatgacc agtcccatat gctcctccag atcgacattg atcagcgact
     5581 catcggactc cggatacatc tccaaatgga ccgaagtcag tttatccggg ccctgtttca
     5641 aattttgata gatctcctcc gccataaacg gcgcaaaggg cgccatcaac ttggcgacat
     5701 tgagcaatac ttcccaaaga gtccggtaga ccgctttttt atgggtatcc atctcggacg
     5761 cccagaaacg atcccgcgac cggcgcacgt accagttgga aacctcgtcg atgacgaatg
     5821 cctgaatctc ccggaccacc cgggtgaggt cataggcttc aatatccgtc cgggcctgac
     5881 ggattaaaga gttcatccgg gaaatgatcc agcggtcaat ttcgtctctg tccgcgaccg
     5941 ggacgttgta gttctcggga tcggcattat cgatattggc atagagcgta aagaaggaga
     6001 atacattctg caaagtcccg aagaatttgg cataggtatc cttgagtccg tcttcatcaa
     6061 accgtgtcgg ggtccagggc ggtgaaaccg ccaacaaata ccaccgggtg gcgtcggcgc
     6121 cgtatttatc aagtagtttc caggggtcta ccgcattccc ccgggatttg gacatcttct
     6181 ggccattctt atccagcagc agatcgttga caagcacccg cttataggta ggcaggtcaa
     6241 agaggaaggt cccgatggca agcaatgaat agaaccaacc ccgggtctga tcaatcccct
     6301 cgcagatgaa atccgccgga aagagcttgt caaaatcatc cttatgctca aagggataat
     6361 gccattgggc gtacggcatt gagcccgagt cgaaccaaca gtcgatcacc tctttagtcc
     6421 gggtcatccg gccgccgcac ccgcatttca aatgaacctc atcaatatac ggccggtgca
     6481 gctcgatggt ggcggggtct actgtttcaa tggcccgttc cgccagttct tgacgggagc
     6541 caaccgacgt cagtttgccg caggactcgc aacgccagat attgagcggt gtgccccagt
     6601 aacggtcccg cgagagcgac cagtcgatca ggttctccaa ccagttcccg aaacgtcctt
     6661 tacccacatg ctccgggtac catttgatct tctggttatt ggcgatcaaa cgttccttgt
     6721 acttggtggt cgcaatatac caggacttac gggcgtaata caacaatggt gtatcacagc
     6781 gccagcagta cggataggag tgagtcatcc tttccttgtg aaacagtttc ccatccacct
     6841 tcagctggcg gatgatatcg gaatcggctt cccgcacgaa ccgtcccgcc caaggcgtga
     6901 tctccggtgt aaacttgcct tccttatcta ccggttgcaa aaccggcaac tgatacttcc
     6961 ggcccaactg gtagtcgtcc tcgccgaagg ccggggcgat atgaacgatt cccgaaccgt
     7021 cctcggtgga tacgaactcc gcgccgtaaa ccttgaaggc cgtggcttca ttcaccttga
     7081 taaagggcag gaattgttca taagactctc ccaccagctt tgatccggga aattcctcaa
     7141 gaatctcggc gcccttgccc aatactgcgt ctacccgatc ccggaccaaa atatagactg
     7201 ccggatcctc gggccggcgc gcccggacat aggtatagct cgggttcacc gccagggcta
     7261 cgttggacgg gagcgtccag ggtgtggtgg tccaaaccag gaaatactcg ccgggtttcc
     7321 cgctcacctg catccggatg taaatggaat cgatggtctc atccttatac ccctgcgaaa
     7381 cctcgtgaga ggccagcggg gttccacagc gcgggcaata cggcatgatc ttatggcctt
     7441 catagatcaa cccttcatca aaataacgct tcaaaatcca ccattccgac tcgatatatg
     7501 agttgtccag ggtgatataa ggatgatcca tatccagcca gtaaccgatg cgctcggtca
     7561 tccgccgcca ttcccgttcg taggtgaaga ccgattctcg gcatttttta ttgaacgcct
     7621 cgatcccgta tttctcaatc tcggtcttat ttgaaagacc caatgacttt tcgacctcaa
     7681 tctccaccgg caggccgtgg gtatcccagc cggccttgcg gttcacctgg tagcccagca
     7741 tggtatgata
//
LOCUS       DASVMB010000114         4625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_160842,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000114 DASVMB010000000
VERSION     DASVMB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160842"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF531_03655"
     CDS             complement(<1..414)
                     /locus_tag="VF531_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713094.1"
                     /translation="MSHKGKVLVAVLVIVLLASVLSVAGLAAPKKVTMWAYAANNIEE
                     WKARKGDIDKKFNINLEIVQIPEQGFVQKLQAAMMDGKNVPDIIEWRVEAPQILFADP
                     KKSFVLPIEKYTSKSKVYKTVMPSRAEWEKYGDHTY"
     gene            complement(918..1886)
                     /locus_tag="VF531_03660"
     CDS             complement(918..1886)
                     /locus_tag="VF531_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713095.1"
                     /translation="MQQIIDELYEFLGFRNHLMFFYLTVSYSIGLPSLVLLILAQAKT
                     RSKALTYMVCLFLTGTLQLILNSFMEYQRVNLPYQVKVYELVFLYTLQGMVIIFLPLF
                     VNEFFSVTYRRRMNRIFCVIFGIGLAFIFIPYLMGMYQGGTEPAFLGEKISIEELTSF
                     KIYRSFIWSAYLYTFIVVTWKIKTVSDFKERFFCIGFFAILFLIFSQTAHPAFKSFPE
                     NLFVSASGNFFLNILLLKYLVDKFFTDFGVFENKSSSRTATEILPLTEREKEVLELLR
                     QGLTNKRIGALLCISEPTVKSHIQNIYKKIGVNNRVQLINSLKNYL"
     gene            complement(1965..2813)
                     /locus_tag="VF531_03665"
     CDS             complement(1965..2813)
                     /locus_tag="VF531_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX7713096.1"
                     /translation="MKKFNRILLVLVLGYSCFSAAILIRILHDYSFFYPVNGHWKLVW
                     EDDFNGPMLDQNKWSPVMQCVNPRQEQQVFLQENVSVRNGCLILASKKEDWTGTDKLH
                     PDQVVTRNYTSGEVGTWEKAVWKYGRFEIRAKLPAGQGVSSYVVLYPNDDTWPPEIHI
                     MVMRGDQPDKIYFANYWGADAKHQRSDNSGPVSVNDYMVNDFSAGFHTFTLEWSPRKL
                     QWYIDDIPKYQVTRNIPDKPLCFVLGTAVGGVFCGDPYDPKFRGIPSSFPQYLIIDWV
                     RVFQRR"
     gene            complement(3322..4038)
                     /locus_tag="VF531_03670"
     CDS             complement(3322..4038)
                     /locus_tag="VF531_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX7713097.1"
                     /translation="MAKLPILYKIQTIDSRLLALKKAEEQIGTDPNRIALTELITQSE
                     QRLGVLREEQQRVSLEQKRIELELKTCQERRKSEETKLYSGSVTASRELELLQQKIAE
                     SAKSQAKQEEMLLHLMEREEELTQEIGENEKRLELARKDLATLEMELKQRQMELAMET
                     EDLRAEQEELLPQIPADWLARYHRIAGSHNGVAVARLKQESCGACHISQSQSQIQKVK
                     RGDDQLLFCENCGRILYYGD"
     gene            complement(4138..4329)
                     /locus_tag="VF531_03675"
     CDS             complement(4138..4329)
                     /locus_tag="VF531_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX7713098.1"
                     /translation="MKQKTKKCEICGSVIPNERLLALPGTKRCVDCSRRKGPDFYAKR
                     TEIGMDPDTYKDLLGATRS"
     gene            complement(4400..>4625)
                     /locus_tag="VF531_03680"
     CDS             complement(4400..>4625)
                     /locus_tag="VF531_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX7713099.1"
                     /translation="LEQNADAYLTGELDYHDVMEASAEGLATIEAGHWTTERGFIPLV
                     AGFLQQTFSEQILEIISDNTTQEEPFQTLV"
BASE COUNT         1240 a         1138 c          972 g         1275 t
ORIGIN      
        1 ataggtgtga tcgccgtatt tttcccattc agcccggctc ggcattaccg tcttatatac
       61 cttggatttg ctggtatact tttcgattgg taatacaaag ctttttttag ggtcggcgaa
      121 taggatctgg ggggcttcaa cccgccactc aatgatgtcc ggaacgtttt taccgtccat
      181 catcgccgcc tgaagctttt gaacaaaccc ttgttcgggt atttgaacaa tttccagatt
      241 aatgttgaat ttcttgtcga tatccccttt acgggctttc cattcctcga tattattggc
      301 ggcgtacgcc cacatcgtga cttttttggg tgcggccaac ccggcaacgg acaagacact
      361 cgccaataac acaattacga gcacagcaac gagcacttta cctttgtgag acatccaaaa
      421 tcctccttca taagattaag taattttgtt ggaaagcaaa gaaccctcac aaatttatac
      481 tcagttccgt tacaagcgaa cgagatctta tccggcccca ccggttaccg gatcttcata
      541 tgtgagtgcc ggaaagcgca ttcctcggat tcgcccgcat caactccttt acaattcagg
      601 tgtataggca tggttaccgg gatgaatcaa cacttaatct ctttaaacca cctattcaaa
      661 atagacattt ccggtaggtt ttacaatccg ccgttcggat gatcttgagc atctcatact
      721 ttcggatgat gttgagagag cgcgtaaata aatcaagaga caggagctat gctcctgtct
      781 cttgatttgg cgccttttga attatctcta gtcgtacttg attgctttcg ttttaaccta
      841 agctgcaagt catgcgtata tatcgcatac aacaagcttt ttctgaagaa tagcatgata
      901 ttgaatcgaa attttaccta aagataattc ttgagtgagt tgatcaattg cacccgatta
      961 ttgactccga tttttttata gatgttttgg atatgcgatt tcaccgtcgg ctccgaaatg
     1021 cataacaagg ctccgattct cttattcgta agcccctgcc gtaaaagctc aagaacctcc
     1081 ttttcccgct cggttaatgg taagatttcc gtagcagttc gcgatgatga tttattctcg
     1141 aatacaccaa agtcggtgaa aaacttatca accagatact tcagcaagag aatattgaga
     1201 aagaaattac ccgaggctga aacaaacaag ttctcgggaa agctcttaaa ggccggatgt
     1261 gctgtctgag aaaaaataag gaaaagaatg gcgaaaaaac cgatacagaa aaacctttct
     1321 ttaaagtcac tcaccgtttt tattttccat gttaccacga taaaagtata tagataggcg
     1381 ctccatatga aagaacggta tattttaaaa ctcgttaatt cctcgatgga tatcttttct
     1441 cctaaaaatg ccggttcggt tccgccctga tacattccca taaggtacgg gataaaaatg
     1501 aatgccaatc caatcccgaa tatcacacaa aatatgcgat tcattcgccg ccggtaagtt
     1561 actgaaaaaa attcgttgac aaacagcggc agaaagatga tgaccatgcc ttgtagggta
     1621 tacaagaaca ccaattcata cactttgacc tgatatggaa ggttgacgcg ttggtactcc
     1681 ataaaggaat tgaggattaa ttggagagtc cccgttagga aaagacaaac catatacgtc
     1741 aatgccttcg atcgggtttt ggcctgggcc aaaattagca aaaccaagga aggcaaaccg
     1801 atggagtacg aaactgtaag ataaaaaaac atcagatgat ttcgaaatcc caaaaactca
     1861 taaagttcat cgataatctg ttgcatcttg tcaaaccctc tccaacacaa actatcaacc
     1921 ggattaacct tccggtttca tagacatgaa agtcagcctc cccattatct gcgttggaaa
     1981 acccgcaccc aatcaatgat caaatattgt ggaaatgagc ttgggatgcc tctaaatttt
     2041 gggtcatacg gatcaccgca gaaaacaccg ccgacagccg tgcccaggac gaaacaaagg
     2101 ggtttatcag ggatatttcg tgtgacttga tattttggaa tgtcgtcgat ataccactga
     2161 agcttgcggg ggctccactc caaagtaaaa gtatgaaaac cggccgagaa atcattcacc
     2221 atgtaatcat tcaccgaaac cggaccggag ttatccgaac gttgatgctt ggcgtccgcc
     2281 ccccaataat tggcaaaata tatcttatcg ggttggtcgc cgcgcattac catgatgtga
     2341 atctccggcg gccaggtatc gtcgttcgga tataagacca cataagatga taccccttgt
     2401 ccggccggaa gtttggccct gatctcaaac cgcccgtatt tccaaacggc tttttcccag
     2461 gtgccgacct caccggatgt ataattcctg gtgacaacct ggtcgggatg gagtttatcc
     2521 gttccggtcc agtcctcttt tttactggca aggatcagac aaccatttct gacggataca
     2581 ttttcttgaa ggaaaacttg ttgctcttga cgggggttaa cgcattgcat taccggcgac
     2641 catttgtttt ggtccagcat cgggccgtta aaatcatctt cccaaacgag tttccaatgg
     2701 ccgttcaccg gatagaaaaa cgaataatca tgcaaaatac gaatgagtat ggcagcactg
     2761 aagcaggagt acccgagaac cagtaccaac agaatacggt tgaatttttt catcggcact
     2821 ccctttctca ctgttgatat tcgtatttgt agaaagatat ttgattatta atatttatga
     2881 taattgtttg ggattcctgc ttcgtccggc gtccatcttt tatggacgct ttctgccggg
     2941 gcatgaattc gacaaagaat ttcaacatcg ccttaagcga aacttttacg aaaggactat
     3001 ataacaaata accctgcatg aaaaatcatc gcagggtttc caatactcac attattttgg
     3061 gatacatggc tgcaagagag tggacaaaga gaaaaaaaaa gggggcattt ccttctacgg
     3121 acaccccgat tcaccgtaaa tcaatttagt aatcaaaccc gcttattttt catcccttcc
     3181 cgcgccccag caaagcttgg ggtgggcgac tacataaaaa atgctgtcgg ttttttaacc
     3241 ccggctaact ggggtgcaaa aaaaagctgt tgctatgaac ttctttgctc cattttgcaa
     3301 caaccccacg tctcaaatcg tttaatctcc gtaatacaag attctaccgc agttctcgca
     3361 gaagagcaac tgatcgtctc ctcgcttaac cttttggatc tgagattggg actggctgat
     3421 gtggcaggcg ccgcagcttt cctgttttag cctggcgacg gcgaccccgt tatgggaacc
     3481 tgcaatccgg tggtagcgag ccaaccagtc cgcgggaatc tggggcaata gttcctcctg
     3541 ctccgcccgt aaatcctcgg tttccatcgc cagctccatc tgtcgttgtt tcagctccat
     3601 ctcgagggta gcgagatcct tccgtgcgag ttcaagtcgc ttttcgtttt caccaatctc
     3661 ctgtgtcagt tcttcctcgc gctccatcaa atgaaggagc atttcctcct gcttagcctg
     3721 ggacttggca gattcggcga ttttttgctg taaaagctca agctcacggg aagccgtaac
     3781 cgacccgctg tagagtttgg tctcttccga tttacgccgc tcctggcagg tcttaagctc
     3841 gagttctatc cttttttgtt ccagtgaaac acgttgctgc tcttcccgga gcacgccgag
     3901 cctttgttcc gattgcgtga tcaattccgt gagcgcgatc cggttgggat cggtcccaat
     3961 ctgctcctcc gccttcttca aggcgagtaa ccggctatca atggtttgaa ttttatacaa
     4021 aataggtaat ttagccatct ctcaataaac tcctaacccg aaaccggcac acaaaccatc
     4081 gccggagctt tgccactacc tttcgataat caccaattat caataacaac tatcttctta
     4141 actccgggtt gctccgagca gatctttgta ggtatcggga tccatcccga tctcggtacg
     4201 cttcgcgtaa aagtccggac ctttccggcg cgaacaatcc acgcaccttt tggttcccgg
     4261 caatgccagt aaacgttcgt tgggaattac gctaccgcat atctcacact ttttcgtctt
     4321 ttgtttcacg atcagtcaac ccccttggtt cgaccgggtt aaccggccga gaatgcggaa
     4381 gggtcatgcc cccgtaaggt taaaccaggg tctgaaacgg ttcttcctga gtcgtattgt
     4441 ccgagattat ctctaaaatt tgttccgaaa aagtctgttg taaaaaaccg gccacgagag
     4501 gaataaaccc tcgctccgta gtccaatgtc cagcctcgat ggttgcgagc ccctccgcag
     4561 atgcctccat tacatcatga taatccagct ctccggtgag ataggcatcg gcgttctgtt
     4621 ccaag
//
LOCUS       DASVMB010000115         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_308114,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000115 DASVMB010000000
VERSION     DASVMB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308114"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF531_03685"
     CDS             complement(<1..320)
                     /locus_tag="VF531_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7713100.1"
                     /translation="MAAKQILFGEEARRALERGVNILADAVKVTLGPKGRNVVLEKKY
                     GSPTITNDGVTIAKEIELGDPFENMGAQLAKEVATKTNDIAGDGTTTATLLAQAMVHE
                     GLKNV"
     gene            complement(376..666)
                     /gene="groES"
                     /locus_tag="VF531_03690"
     CDS             complement(376..666)
                     /gene="groES"
                     /locus_tag="VF531_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7713101.1"
                     /translation="MNIRPLGDRVVLQVLESEERTKSGIVLPDTAKEKPQMGKVLAVG
                     EGRLLENGMKHALDVQVGDKVLFTKYAGTEIKLDGEEYMIMKEIDILGIVAG"
     gene            994..1935
                     /gene="ligD"
                     /locus_tag="VF531_03695"
     CDS             994..1935
                     /gene="ligD"
                     /locus_tag="VF531_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX7713102.1"
                     /translation="MLPDHLRPMLAKLSHPFDSAEFHYEIKWDGYRCLAFLDSATRLQ
                     SRNLKELSGFFPELSRLHLQCSRQGCLLDGEIVTFRNGLPSFQRLQKRAQLRSMEQIQ
                     AAARSAPVVYIAFDLLYLDGEPVYHTPWKERRSLLSDLVRPTESFILSEAIPNRGVDF
                     FQAVATKGLEGVMAKRVDSPYLPGKRSDFWLKFKSRHRGYFLICGFLLQPKDCKLRSL
                     ILGAFQDEELQLRGTVGSGLPPSELDTIKQLLEKLATEICPFTGTAIKKKDTIWIEPR
                     LVCEVEYLELTDEGYLRHPSFKNFRPDLQAGACRLED"
     gene            1936..>2209
                     /locus_tag="VF531_03700"
     CDS             1936..>2209
                     /locus_tag="VF531_03700"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX7713103.1"
                     /translation="MEQTVMIHQNRIPVTNLDKLFWTAEGITKADIMKYYAAVWPFLE
                     PHLKDRPVSLVRYPEGVSGKFFYQKDVPDPPDWVATLPLASEDRVIN"
BASE COUNT          533 a          578 c          485 g          613 t
ORIGIN      
        1 acatttttaa gcccctcatg caccattgcc tgggcaagta atgtggctgt ggtcgttccg
       61 tctccggcga tatcattggt cttggtggct acttctttgg ccagttgcgc tcccatattt
      121 tcaaagggat ccccaagttc gatctctttg gcgatggtta ccccatcatt ggtgatggtg
      181 ggagagccgt acttcttctc caataccaca ttgcggccct tagggccaag agtgaccttg
      241 accgcgtcgg ccaggatatt gactccgcgt tccaatgcgc gacgggcttc ttccccaaac
      301 aagatctgtt ttgctgccat tggtatcacc ttcctttttt taggatgtca ttttctttta
      361 cctccggttt ccccttcatc cagctacaat accgagaata tcaatttcct tcatgatcat
      421 atattcctct ccgtctagtt taatttcggt accggcatat ttggtgaaca gaaccttatc
      481 gcccacttga acatccaacg catgcttcat accattctca agtaatctgc cttccccaac
      541 cgccaatacc ttacccattt gcggtttctc tttcgcagta tccggtagga cgatgccgct
      601 cttggtccgc tcttcgcttt ccaggacctg taacacgacc cgatctccca atggcctaat
      661 attcatcaat atcaccccct aatctcttga gataatcagc actctttaat tgtgagtgct
      721 aaattccaac cacaatctta taaaaatgta aacatcttgg caagattcca aaatttaaat
      781 ataggcctat ttttactcca atttgaaagg tttataccct tagcccggct acagttgcac
      841 cgggctagcg aattcaggca gaatgtaccc gatacctttt cctaactatt tggacctgta
      901 tttccacggg aatgattttg ggaatgggga atgctatcaa aagaaaccac ccccacatgt
      961 gcacgtaatc agcccaaacc gaggttttta taaatgctcc cggatcatct ccgaccaatg
     1021 ttggctaagc tttcgcatcc cttcgactct gcagaatttc actatgagat caaatgggat
     1081 ggataccgct gtcttgcctt tctggacagc gctacccggt tacaaagccg gaacctgaaa
     1141 gaacttagcg gatttttccc ggagttatcc cggttacacc ttcagtgcag caggcaaggt
     1201 tgtttgctgg acggggaaat tgtgaccttt cgtaacgggt tacccagctt tcagcgatta
     1261 caaaaacggg ctcagcttcg cagcatggaa cagatccaag ctgccgcccg ttctgctccc
     1321 gtggtctata ttgcctttga tctgctctac ctggatggag aaccggttta tcatacacca
     1381 tggaaagagc gtcgcagttt attgtcggac ttggtccggc ccacggaatc atttatccta
     1441 tctgaagcaa ttcccaaccg gggagtggac ttttttcaag cagtcgcgac caaaggcctg
     1501 gagggagtga tggctaaacg ggtcgacagt ccctatctac ccggcaagcg gagtgacttt
     1561 tggttaaaat ttaaaagtcg acacagggga tactttttaa tctgcgggtt ccttttacaa
     1621 cctaaagatt gtaaactgcg ttccctgatt ctcggcgcct ttcaggacga agaacttcag
     1681 cttcggggga cggttggcag cggtcttcct ccctccgagt tggataccat taaacaacta
     1741 ctggagaagc tggcgaccga aatctgtccg ttcaccggga ccgctattaa aaaaaaggat
     1801 actatttgga ttgagccccg attggtatgt gaggtggaat acctggaact aaccgacgaa
     1861 ggatatctac ggcatcccag ttttaaaaat ttccgccctg acctacaggc gggggcctgt
     1921 cgtttggagg attaaatgga acagacagtc atgattcacc aaaaccggat tcccgtaacc
     1981 aatctggata agctcttttg gactgcggaa ggtataacca aggccgatat catgaaatac
     2041 tacgctgcag tctggccatt tcttgaaccc catctaaaag accgcccagt ttccctggtt
     2101 cgttatccgg agggtgtttc ggggaaattc ttctatcaaa aggatgtacc ggatcctccg
     2161 gattgggttg caacactgcc cctcgcatcc gaggaccggg tcatcaatt
//
LOCUS       DASVMB010000116         9190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_237173,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000116 DASVMB010000000
VERSION     DASVMB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237173"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VF531_03705"
     CDS             complement(<1..460)
                     /locus_tag="VF531_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713104.1"
                     /translation="MPSTIAIIEDEPNIVELVKYNLDREGYRTISAGTGKKGLELVHQ
                     ELPDLVILDLMLPELDGMSVCKQLRSDPQTKAIPIIILTAKSEEADRVLGLEMGADDY
                     VTKPFSPRELIARIRAVLRRSTNVEEGEPEVIEIGEIRMDLRQHLVKVRGQ"
     gene            complement(700..1410)
                     /locus_tag="VF531_03710"
     CDS             complement(700..1410)
                     /locus_tag="VF531_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEX7713105.1"
                     /translation="MLEVLKNQVLEANLELQNHHLVVYTWGNASGIDRESGLIVIKPS
                     GVPYGELRADHMVVLDLDGRIVEGTLNPSSDTPTHIELYRSFPEIGGVVHTHSSWATA
                     WAQAGVGIPCLGTTHADYFYGEIPCTRPLTTGEIRNDYELNTGKIIAECFTKLDYRQI
                     PGVLVTNHGPFSWGKDPAEAVYHSVVLEEVAKTGWATLGLRSITTRRITGIPQELLDK
                     HFLRKHGRNAYYGQKDHS"
     gene            complement(1504..3201)
                     /locus_tag="VF531_03715"
     CDS             complement(1504..3201)
                     /locus_tag="VF531_03715"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEX7713106.1"
                     /translation="MSENQGQLVLGIDFGSDSVRAVAINAVTGREEANEVAYYKRWAE
                     GRYCNPSRNQFRQHPLDYIEGLETVIKGALAKLPSGAGTRVAGIGIDTTGSTPCPVDR
                     TGMPLALKPEFADNPNAMFILWKDHTAIQEAEEINHLARSWGGTDFTQYEGGVYSSEW
                     FWAKILHVLREDPAIRGAAFSWVEHCDWIPALLTGTAEPLTMKRSRCAAGHKAMWHQD
                     WGGLPNEAFLVKLDPLLKDLRNRLFHDTYTADVNVGGVTPEWAARLGINPGTAVAGGA
                     FDCHMGAVGGNIMEATLVKIMGTSTCDVMVASPEALAGKRVAGICGQVDGSVLPGMIG
                     LEAGQSAYGDVYAWFRRLLSWPLAVVLPEIDIPGGVDKAMTGKIREAIESKLLVKLAE
                     AAEQLDPADTGLIALDWLNGRRTPFADQALKGALIGLNLGSDAPRVYRALVEATAFGA
                     KAIVERFQEEGVAIEQVIAMGGIAKKSDFVMQVTADVLNMPIKVTQSEQACALGAGIF
                     AAVAAGLYPSVAAAQKKMSSGFSKTYEPRPKEAEQYRKLYQKYLQLGQTLADQLRSL"
     gene            complement(3342..4442)
                     /locus_tag="VF531_03720"
     CDS             complement(3342..4442)
                     /locus_tag="VF531_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7713107.1"
                     /translation="MPAPNHNDFSPKHQRLKDYLLHMMQTGAIAPGEQLPSENLLAEQ
                     HQVSRHTVRQALGALENEGWICREQGRGTFCANPADRSAGRKIAVMTTYISDYIFPGI
                     IRGIEEVLSASGYTLILTNTHNDLIKEAQCLEKLLSQGINGLIIEPTRSAIPNPNLTY
                     YRRLEERHLPYLLIHAVYPDLDPAYIIMDDVKGGYLATQYLLQLGHRRIAGIFKQDDL
                     QGLNRKRGFTQALSEYGITVEPALAGNFQTEQLSSYPFSFTQRLLQSPDPPTAIVCYN
                     DQVALKVLEATRMIGLKVPEDISLIGYDDSSLAVATEVKLTTVRHPQVEMGRQASRLL
                     IEMLERQVDRPHFIYPPELIIRDSCRSLPHSG"
     gene            complement(4622..5557)
                     /locus_tag="VF531_03725"
     CDS             complement(4622..5557)
                     /locus_tag="VF531_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7713108.1"
                     /translation="MAYNLKEIAKKPERLSGGHRMCAGCGAPVVVRQILRALRPEDHA
                     VIGSATGCLEVTTFLYPYTAWKDSFIHNAFENSAASISGAEAAYRALKRKGKLARETK
                     FLAFGGDGGTYDIGLQSLSGAMERGHDMVYVCYDNEAYMNTGIQRSSATPKYADTTTS
                     PVGKKVPGKQQWRKDLTEIMVNHHLPYVAQTAAVGNMKDLYEKAEKAIYTQGPCFLNV
                     LAPCPRGWQYNTYDLMEINRLAVETCFWPLYEVSEGKYILNYRPKNKLPVRDFLGMQG
                     RFKHLFKAGNEYLVDEIQTEVDKRWERLLELSGEV"
     gene            complement(5570..6754)
                     /gene="porA"
                     /locus_tag="VF531_03730"
     CDS             complement(5570..6754)
                     /gene="porA"
                     /locus_tag="VF531_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX7713109.1"
                     /translation="MAIRERMSGNEATAFAMKQINPDVVAAFPITPSTEVPQYFSNFV
                     ADGTVNTEFVAVESEHSAMSACIGAQAAGGRAMTATSANGLALMWECLYIAASSRLPI
                     VMACVNRALSGPLNIHNDHSDSMGARDSGWVQLYSENNQEAYDNMLMAHRIAEDPRVM
                     LPVMVCQDGFITSHAVENIELVEDDQVKTFVGEYKPKYYLLDRENPVSVGPLDHFTHC
                     FEHKRQQAQAMANARQVILEVASEFEKITSRSYGFFEEYQTADAVVVAVMMNSTAGTA
                     KFVVDRLRAEGKKVGLIKLRVFRPFPVNELTAALSKFKAVAVMDKADSFNAAGGPLFT
                     EISSAIFGKGISGVKVINYIYGLGGRDVKPGDIEYVYGELLKIASSGQVESVYNYLGV
                     RE"
     gene            complement(6768..7058)
                     /locus_tag="VF531_03735"
     CDS             complement(6768..7058)
                     /locus_tag="VF531_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7713110.1"
                     /translation="MVPNDVTWREITIGGTIPYAGNASLFKTGDWRSMKPRWIQEKCK
                     QCLLCYPVCPDTSILIDAEGKRVEFDYDHCKGCGICAKVCPFKAIEFVEESK"
     gene            complement(7088..7666)
                     /locus_tag="VF531_03740"
     CDS             complement(7088..7666)
                     /locus_tag="VF531_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX7713111.1"
                     /translation="MGKSVEIRWHGRGGQGAKTASLLLAEAAFNTGKYIQGFPEYGSE
                     RMGAPITAYNRISDDKLTIHCNVYEPDYVAVVDDTLLASVDVTAGLKKSGAIIINTTK
                     SPDEIRPLLKGYPGKICTIDARSISIETLGKYFPNTPMLGAVVKVSKVLEEEEFLKDM
                     VDSFKHKFAKKPEVVEGNIHALERSLREVKGL"
     gene            complement(7696..8307)
                     /locus_tag="VF531_03745"
     CDS             complement(7696..8307)
                     /locus_tag="VF531_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7713112.1"
                     /translation="MDAAKTSTKDRIIESSIDLFSEKGFYETSIREIAKKCNLQVSSL
                     YSHFSGKEEILDDILKIYREELVKVRIPDERIESLVNSMSLEEILAKGFTVLIDTTSS
                     LRMNKIIKIMVMEMYRNPKVRDFYTQWYFNENRISIMNLFKRLQAQEKIKKVDLEMLT
                     SVFGALMNYFYHEYFLAKLDHKDTKELEQKIEQHLEFFVGLIK"
     gene            complement(8565..9053)
                     /gene="ybaK"
                     /locus_tag="VF531_03750"
     CDS             complement(8565..9053)
                     /gene="ybaK"
                     /locus_tag="VF531_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX7713113.1"
                     /translation="MAKEKFPVTPAIRVLRAAGVDFQEYLYEYEERGGTAIASRELGI
                     DEHLAIKTLIMEDENHNPLVVLMHGDREVSTKELARIIGVKSVTPCTPENASKHSGYL
                     VGGTSPFGTRRKMPVYVEETILDLPVIYINGGKRGFLVAMNPEELNRLVDPKPVRVGI
                     KE"
BASE COUNT         2219 a         2496 c         2037 g         2438 t
ORIGIN      
        1 cctggccgcg aaccttgacc agatgctggc gaaggtccat ccggatctcg ccgatctcaa
       61 taacttcagg ctcgccctct tccacattgg tgctgcggcg aagtaccgcc cgaatccggg
      121 cgattaactc acggggtgaa aacggtttgg tgacatagtc gtccgcaccc atttccaatc
      181 ccagaaccct gtctgcctcc tcgctcttgg cggtaagaat gatgatcggt atcgccttgg
      241 tttgaggatc ggaacgtaat tgcttgcaga cggacattcc atcgagttcg ggcaacatca
      301 agtcgaggat gaccagatcc ggcagttcct gatggactag ttccagacct ttcttaccgg
      361 ttcctgcgga aatggtacga taaccctcgc ggtccaggtt gtatttgacc aactccacaa
      421 tattgggttc gtcttcgata attgcaatcg tactgggcat attgaatcct cctactccat
      481 tggtttgcga taaggaaact ccggccctca ttatgatgag cctttggggg atagccgggc
      541 ttgtccagtc aaccccaaat aaaaccggat cttcattcag ttcattatat caaaaaaaag
      601 gttttatgcc cagtggttta gaaacctcga tccggcaaat acactgcaat ttccttacgg
      661 tgacgcagaa aaattggctg gatcgggttt tctacagtgt cacgaatggt ctttttgacc
      721 gtaataagcg ttccggccgt gtttacggag aaaatgctta tccaagagtt cctgaggaat
      781 gcctgtaatt cgcctggtcg taatggaacg taatcccaag gtggcccaac ccgtctttgc
      841 gacttcctcc agaaccacgc tatgatagac tgcttcggca ggatccttgc cccagctgaa
      901 cggaccgtga ttcgtcacta aaaccccggg tatctggcga taatccaatt tcgtaaaaca
      961 ttctgcgatg atcttcccgg tattcagctc gtaatcgttc cggatctccc cggttgtcaa
     1021 cggacgggta cacggaatct ccccgtaaaa ataatcggca tgagtcgtac ccagacaagg
     1081 tatacccacc ccggcctgag cccaggcagt ggcccaggac gaatgcgtat gaactacacc
     1141 cccgatctcc gggaaactac ggtacagttc aatgtgagtt ggcgtatccg aagacgggtt
     1201 tagggttccc tccacaattc ggccatccaa atccaaaacc accatgtgat cggcccgcag
     1261 ttccccgtaa ggaacgccgc tgggtttaat gactatcaag cccgactcgc gatcgattcc
     1321 actggcgttt ccccaggtgt ataccaccag gtggtggttt tgtagttcca gattggcctc
     1381 aagcacttga tttttcagta cctcgagcat atcccaggct cctttcagcc tttatcccgg
     1441 agattggaag acacggcata accaatccca tcccccggcg aaatgtgaat aacgcgaccc
     1501 gaactaaagc gacctcaatt gatctgccaa ggtttggccc aattgaaggt atttttgata
     1561 caacttccgg tattgttcgg cttccttagg acgaggttca tatgttttac tgaatccgct
     1621 gctcatcttc ttttgagcgg cagcaaccga aggatataaa ccggcagcta cagcggcaaa
     1681 gattccggca cccaaagcac aggcttgttc ggattgagtc accttaatcg gcatattcaa
     1741 gacatctgct gtaacctgca ttacgaaatc gctctttttg gcgatgcctc ccatggcaat
     1801 cacctgttca atggcgactc cctcttcctg gaatctctct acaatcgcct tcgctccaaa
     1861 agctgtggct tccaccaacg cgcgataaac acggggtgca tcgcttccca ggttcaatcc
     1921 gatcaatgcg cctttcaagg cttgatccgc aaaaggagtt cgccggccat ttagccaatc
     1981 aagcgcgatt aacccggtat cggccggatc taattgttcc gctgcctccg ccaattttac
     2041 gagcagtttc gactctatcg cttcacgaat tttaccagtc atagctttat ctacgccccc
     2101 cggtatatcg atctccggaa gcactaccgc taaaggccac gacagcagtc tgcggaacca
     2161 ggcgtaaaca tcgccataag ctgattgacc cgcctccaga ccgatcatcc cggggagaac
     2221 cgagccgtcg acctggccac agattcccgc aactcgtttt ccggccaaag cctctggtga
     2281 cgcgaccatg acgtcacaag tggaagtccc catgatcttg accaatgttg cttccatgat
     2341 gttcccgccg acagctccca tatggcaatc aaaggcgccg cccgccactg cggttccggg
     2401 attgatgcca agacgcgcgg cccattcggg ggttactccg cccacattga catctgcggt
     2461 atatgtgtcg tgaaacaaac ggtttctcaa atctttgagc agaggatcca atttcaccaa
     2521 aaaggcttca ttcggcagac cgccccaatc ctgatgccac atagccttat gccctgcggc
     2581 gcagcggctt cgtttcatgg ttaaaggctc ggcggtacca gtgaggaggg ccgggatcca
     2641 gtcgcaatgt tcaacccacg aaaaggctgc tccacgtatg gccgggtctt cccgaagcac
     2701 atggagtatc ttggcccaaa accattctga agagtagaca cccccttcat actgggtgaa
     2761 gtcggttccg ccccaactcc tggcgagatg gttgatctct tcggcttcct ggatagcggt
     2821 gtgatccttc cagagtatga acatggcatt ggggttatcc gcaaattccg gtttcaacgc
     2881 cagcggcatt ccggtgcgat caaccgggca aggtgtcgaa ccggtggtgt cgatcccgat
     2941 ccctgccacc cgggtccccg ctcctgatgg taatttagct aacgcacctt tgattaccgt
     3001 ctccagtccc tcaatatagt ccaagggatg ctggcggaac tgattcctgg acggattgca
     3061 ataccttccc tcggcccaac gtttgtaata agccacttca ttggcttcct ccctgccggt
     3121 gaccgcattg atagccaccg cccgtaccga atcgctcccg aaatcaatac ccagtactag
     3181 ttgcccttga ttttcactca tgccaatcct ccttttgcct tatgattttg aataatctac
     3241 ctatccgcaa ccacagttcc gttcaaatgt acgtacaacc cgatggaaaa atagccctta
     3301 tgtgcaaatg aataaacatg gcttcccgtt ctcccagcgt gtcaaccact gtggggtaaa
     3361 gaacggcatg aatcgcgaat gattaattcc ggtggataga taaaatgcgg ccgatctacc
     3421 tgcctttcca gcatctcgat gagcaaacgc gatgcctgac gacccatctc cacctgagga
     3481 tgacgaacag ttgtcagttt tacctcggtg gccacagcca ggcttgaatc atcatatccg
     3541 atcaaggaga tgtcctccgg gacctttaat ccgatcatcc gagtagcctc caacactttc
     3601 agggcgactt ggtcattgta acaaacgatc gctgtcggag ggtcggggct ttgcagcaat
     3661 ctttgggtaa agctgaatgg ataggatgat aactgttcgg tctgaaaatt tcctgccaaa
     3721 gcaggctcaa ccgttatgcc gtattccgag agcgcttggg tgaaaccccg tttgcggttg
     3781 aggccctgca gatcatcctg tttaaaaatc ccggcaatac ggcgatgccc cagctgaagc
     3841 aggtattgag tcgcgagata gccgcctttg acatcatcca tgataatata ggcagggtct
     3901 aaatcggggt atactgcatg aatcaacaga tagggtaaat ggcgttcttc cagacgccgg
     3961 taataagtaa ggttgggatt gggtatggca ctgcgggtcg gttcaatgat caacccgtta
     4021 atcccctgac tgagtaattt ttccaggcat tgggcctctt tgatcaggtc gttatgagta
     4081 ttcgtcaaga tcaatgtata accggaagcg ctcaaaacct cttcaatccc tcgaatgatc
     4141 ccgggaaaga tgtaatcgga gatataggtg gtcatgaccg caatcttccg gcccgcggat
     4201 cgatccgcgg gattggcgca aaaagtaccg cgtccctgtt cacgacagat ccagccctca
     4261 ttttccaggg cgcccagcgc ctggcgtacc gtatgccggc tgacttgatg ctgttcggcc
     4321 agaagatttt cggatggcag ttgttcaccg ggtgcaatcg ccccggtttg catcatgtgt
     4381 aataaataat cctttaaccg ctggtgcttg gggctgaaat cattatgatt cggggcgggc
     4441 attaactttt tcacctattt gccaaattgt acgtacctat tatttttcta ttcgacatcg
     4501 gaaaaattta tcctgcgacc agatttgttc cttcatataa atgcccggat tgaactttta
     4561 taaatgatgg tatacattta aagcagcttc agcagcttct tcccatcaaa aatgctaccg
     4621 cctaaacttc gccggagagc tccaatagtc tttcccaacg tttatcgact tccgtctgta
     4681 tctcatctac caaatactca ttaccggcct tgaaaaggtg cttaaacctt ccttgcattc
     4741 caaggaaatc ccgcaccggg agtttgtttt tcgggcgata atttaatatg tattttcctt
     4801 cgctaacttc gtataacggc cagaaacagg tttccaccgc caacctgttt atctccatta
     4861 gatcgtaggt gttgtactgc cagccgcggg gacacggggc cagcacgtta agaaaacacg
     4921 ggccttgcgt atagatggcc ttttccgcct tttcgtaaag atccttcata tttcccactg
     4981 ccgcggtttg ggcgacgtac ggcagatgat ggttaaccat gatttcagta agatccttgc
     5041 gccattgctg ttttcccggg acctttttcc caacgggcga ggtggttgta tcggcatatt
     5101 tcggagtagc cgatgaacgc tgaatgccgg tgttcatata agcctcattg tcatagcaaa
     5161 cataaaccat gtcatggccc cgctccatcg ctccggacag tgactgcagg ccaatgtcat
     5221 aggttcctcc atcgccgcca aatgctagaa atttagtttc ccttgcgagc ttcccctttc
     5281 gctttaaagc tcgatatgcg gcctccgcac cggatatcga agcggccgaa ttttcaaagg
     5341 cattgtgaat gaatgaatcc ttccatgcgg tataaggata aagaaatgta gtcacttcca
     5401 aacatccggt ggcgcttccg ataaccgcgt gatcttcagg ccgtaaagcc cttaatatct
     5461 gtctgacgac aaccggagcg ccgcatccgg cgcacattct atgaccaccg ctgagccttt
     5521 ccggtttttt tgcaatctcc ttcagattat aagccattca ttattcctcc tattccctta
     5581 cacccaggta attatagact gattcaacct gaccggagct tgcgatcttc aacaactcac
     5641 cgtatacata ttcgatatct ccgggcttga catcccttcc gcccagacca tagatgtaat
     5701 taatgacttt cacgccggat atcccttttc caaaaatagc gctggagatc tcggtaaaca
     5761 acggcccacc ggcggcatta aaactgtcgg ctttatccat taccgcaacc gccttgaact
     5821 tggataaagc ggcagttaat tcattgactg gaaaaggtct gaacaccctg agtttgatta
     5881 gaccgacttt ttttccctcc gcccgcagcc ggtctacgac aaatttggct gttccggcag
     5941 tagagttcat catcacggca accactacgg catccgccgt ttgatactct tcaaaaaagc
     6001 cgtagcttct actggttatc ttttcgaact cggatgcgac ttccagtatg acttgcctgg
     6061 cattcgccat tgcctgggct tgttgtcttt tatgttcgaa acagtgggta aagtggtcca
     6121 aaggccccac cgaaacaggg ttttcccgat ccagaagata gtattttggt ttatactctc
     6181 cgacaaaggt cttaacctga tcatcctcta ccaattcgat gttttccacc gcatgggaag
     6241 tgataaaacc gtcctgacaa accattaccg ggagcatcac ccggggatcc tcggcaatcc
     6301 tatgggccat cagcatattg tcgtatgctt cctggttatt ttcgctataa agctgaaccc
     6361 agccggagtc ccttgcgccc atggaatcgc tatggtcgtt atggatgttt aacgggccgg
     6421 aaagtgcccg gtttacacaa gccattacaa ttggtagtct ggaactggcg gcaatataca
     6481 gacattccca cataagggcc aggccattcg cagatgtagc ggtcatcgcc cttccacctg
     6541 ccgcttgagc tccaatacat gccgacatgg cgctgtgttc cgattcaacg gcaacaaatt
     6601 cagtatttac tgttccgtcg gcaacaaaat ttgagaaata ctgaggaact tcggttgatg
     6661 gggtaatcgg aaatgccgcg actacatccg gatttatctg cttcatggca aaggctgtgg
     6721 cttcgttacc gctcattctt tccctgatgg ccattattac atcctcctca cttgctttcc
     6781 tctacaaatt caatggcttt aaatggacag accttggcgc atatgccgca acccttgcaa
     6841 tgatcgtaat caaattcaac ccttttacct tccgcatcga ttaatataga tgtatcgggg
     6901 catacagggt aacaaagcag gcactgcttg catttttctt gtatccaccg tggtttcatt
     6961 gaacgccagt ccccggtttt aaataaactg gcatttccgg catatggaat ggtaccgcct
     7021 atggtaatct ctctccaggt tacatcattg ggaaccatac gcgattcggt tttttttatg
     7081 tagtcattca cagacccttt acctccctta gcgaccgttc gagcgcatgg atattgcctt
     7141 ctaccacttc gggtttttta gcaaatttat gtttgaagga atcgaccata tcctttaaga
     7201 actcttcttc ctcaaggact ttgctgacct ttacaaccgc gccgagcatc ggggtgtttg
     7261 gaaagtattt tcccaaagtt tcaatggaga tgctcctggc gtcgatggtg caaatcttcc
     7321 ccgggtaacc ttttagcaaa ggccttattt catcgggaga ttttgtggta ttgatgataa
     7381 ttgctccaga cttctttaac cccgccgtaa catcaaccga agcaagcaaa gtatcatcca
     7441 ccaccgcaac ataatccggt tcatacacat tgcaatggat cgtcagttta tcatcactga
     7501 ttcggttata cgcggttatc ggcgccccca ttctttccga gccgtactcc ggaaagccct
     7561 gaatatactt gcccgtatta aacgccgcct ccgccagcag cagggacgca gtcttggcgc
     7621 cttgcccgcc gcggccgtgc catcgtatct caacgctttt acccatcgaa tgaccccctt
     7681 tggctatccc gggttttact taattaagcc gacaaagaac tctaggtgtt gttcaatctt
     7741 ctgctccaat tctttggtat ccttatgatc caacttggcc aaaaagtact catgataaaa
     7801 atagttcatc agcgcaccaa agaccgaagt aagcatctcc aggtcaacct ttttgatctt
     7861 ttcttgcgct tgcaacctct taaatagatt catgattgaa atcctgttct cattaaaata
     7921 ccattgggta taaaaatcgc gcactttcgg attacgatac atctccatta ccataatctt
     7981 gattattttg ttcattctga gcgaagaggt ggtatcaatg aggacggtaa aacctttggc
     8041 caggatctcc tcgagagaca tactgttcac aagcgattcg attctttcat cgggtattct
     8101 caccttaact aattcttcac ggtaaatttt gagtatgtca tctaaaatct cttccttgcc
     8161 ggaaaaatga ctatatagag aacttacttg gagattacac tttttggcga tctcgcgaat
     8221 cgaggtctca tagaaaccct tctcggaaaa gagatctatt gacgactcga tgattctatc
     8281 ttttgtggaa gttttggcag cgtccatggc ttcacctcaa tgatatggca aacaaaacga
     8341 acgatcgttc gtatttatat taacatacga tcgttcgttc gtaaagcgtt atttgtttaa
     8401 ttgtgcctca ctcagtgccc cattacaaaa accacgaaac acacgaaatt tcacgaaaaa
     8461 aggagcatag gatcaaccat ttcgaaaaca tagcgaaaag tttgtttttc ctcgtattac
     8521 cccttggaga gttaaagagc cgattttaaa tcccagtaca cagtttattc cttgatcccc
     8581 acccggaccg gtttcggatc gacgagccgg ttcagttcct ccgggttcat agccaccaga
     8641 aacccccgtt tgccgccgtt aatgtatatc accgggagat ccagaatggt ctcttctacg
     8701 tacaccggca tctttcgcct ggtaccaaag ggtgaggtgc cgcccaccag atagccgctg
     8761 tgtttactgg cattctccgg cgtacagggg gttaccgatt tcactccgat aatccgtgcc
     8821 agttccttgg tggaaacttc acggtcgcca tgcatcagga cgaccagcgg gttatgattt
     8881 tcatcttcca taatcagggt tttgatcgcc aaatgctcat ctatacccag ctcccggctg
     8941 gctatggcgg tgccgccccg ctcttcgtat tcgtaaagat attcttgaaa gtcaaccccg
     9001 gcggcgcgta acacccggat ggccggagtg accggaaatt tttctttcgc catgacggga
     9061 atcctttcga aaaataatta tacaaaaaaa tccattatct cccggggttc cgcgattaat
     9121 cgcttcgcgc cgctcgcctc aagctccgac gcggtccgga acccccacag cgcaccgacc
     9181 ggatacatcc
//
LOCUS       DASVMB010000117         9235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_382795,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000117 DASVMB010000000
VERSION     DASVMB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382795"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VF531_03755"
     CDS             <1..653
                     /locus_tag="VF531_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713114.1"
                     /translation="AGKFETTVKDKYKDYFNEVKGNNSGATLIGLRGWGNGPTTKPDN
                     ENLKGQNTYDDMLLVLGEDGKLGAFSAVNFESTDAKGWYKSKGEKKTLTGDYNDIGDV
                     TAEVNYGYHGDYQALWLRNNYPVKGTKPNGEMTFLEGIHIHKGGDTWTFSKGCITIYD
                     GSNPNFSGQWDRFMSYFKINSPIIDPDTNQPVVDSLGRTEGEISRQRAGSISLMRL"
     gene            666..1430
                     /locus_tag="VF531_03760"
     CDS             666..1430
                     /locus_tag="VF531_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713115.1"
                     /translation="MRKIFLIITFILFTLSVTTSANNLDIDKSILNIIKSIGIDASEV
                     ELELEPNELISYEEYSKKDLYEQKTNEAAWSTIWHDNLEKLDVLTKGSLTFKGYLTNN
                     LFDNKVDTAWVEGANGDGIGEWISIVLNAKRTDRPTGMLYFGMIPGYLKSDKIWKENN
                     RVKTALLIVERYTGYDIRTKSFRYDYPIIRLKFKDFKGLQVFNLLDYGAPPTYDPKRL
                     WVIIENVYKGTKYNDTCISEIVVSGTCAPGEDEGEE"
     gene            complement(1506..1757)
                     /locus_tag="VF531_03765"
     CDS             complement(1506..1757)
                     /locus_tag="VF531_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713116.1"
                     /translation="MEDNKVAVLLEDLIAKFRVFGEGLDGLRDEMNNRFSEMNNCFNN
                     LEQKVDKLESKLDAHIEENHRDHLQLMQLIKETDIEVQE"
     gene            2276..2959
                     /locus_tag="VF531_03770"
     CDS             2276..2959
                     /locus_tag="VF531_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX7713117.1"
                     /translation="MVTDENGNKVVERDFTPFGQRIDQAGHEGKFPEETEDGFTGKDW
                     DEDIGLYYFNARWYDPEVGRFISEDSVADDPNLYSYCGQNPVNNVDPTGNFSIGLQQG
                     WNMVGSMINATALLSKNETLGMISSVFSLFVAIDGRWGLSNIMDDITDLFSGNSFEKQ
                     LHKNISGYIDSKFELESIARDPDELYGAILNINRSKSGSVDSGKNGIESSTATGSMDF
                     QQKKIKKRK"
     gene            complement(2997..3140)
                     /locus_tag="VF531_03775"
     CDS             complement(2997..3140)
                     /locus_tag="VF531_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX7713118.1"
                     /translation="MSSSVDKALWENFQQLSKKTRIPVSRLLDEAIEDSLKKYNQTQK
                     SSN"
     gene            3292..3498
                     /locus_tag="VF531_03780"
     CDS             3292..3498
                     /locus_tag="VF531_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HEX7713119.1"
                     /translation="MRNHRYVTVGSVHRNNKLVPLIRLSGSWLAENGFKTGRKVHIYV
                     KPGSLTVNLILPEDEEEYRIQPLE"
     gene            complement(3551..3787)
                     /locus_tag="VF531_03785"
     CDS             complement(3551..3787)
                     /locus_tag="VF531_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713120.1"
                     /translation="MEDNKVAVLLEDLRAQFRAFGEGLDVLHHEIKEDRQEMADFRNE
                     TNQRFLENSRDHQQLMQMIKELDKEVQVEIKRVK"
     gene            complement(4108..4332)
                     /locus_tag="VF531_03790"
     CDS             complement(4108..4332)
                     /locus_tag="VF531_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713121.1"
                     /translation="MEDNKVAVLLEDLRAQFRVFGESLQMLNEKMDRGFKEIKDEIRE
                     FKEQNRQEHQQLQQAIAELDTEVVKLKRVK"
     gene            complement(4553..4798)
                     /locus_tag="VF531_03795"
     CDS             complement(4553..4798)
                     /locus_tag="VF531_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713122.1"
                     /translation="MEDNKVAVLLEDLRAQFGVFGEGLQLLDSKVDKGFQEVNEKIDS
                     LQTELANFKDENRQEHQQLQQAIAELDTEVVKLKRVK"
     gene            5144..5602
                     /locus_tag="VF531_03800"
     CDS             5144..5602
                     /locus_tag="VF531_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713123.1"
                     /translation="MDSDVMIREATDADIEAMVRLLRVLFTIEADFHVNEAAQRRGLA
                     MMLEDSDHRRVLVAEVEGSISGMCTGQALISTAEGGMAVLVEDLVVLEAYRGYGIGSR
                     LLAAMEAWGFGIGARRLELLADRENRPALDFYQRKNWRQTRLVCLHKKEA"
     gene            5867..6691
                     /gene="nifH"
                     /locus_tag="VF531_03805"
     CDS             5867..6691
                     /gene="nifH"
                     /locus_tag="VF531_03805"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HEX7713124.1"
                     /translation="MRKIAIYGKGGIGKSTTTQNTVAGLAEMGRKVMVVGCDPKADST
                     RLLLGGLAQQSVLDTLRDEGDDIDLGDVMKSGFASTLCVESGGPEPGVGCAGRGIITS
                     INLLEQLGAYEEDKNLDYVFYDVLGDVVCGGFAMPIREGKAQEIYIVVSGEMMAMYAA
                     NNICKGIVKFAESGGVRLGGLICNSRKVDNEEALIRAFAAQLGTQMIYFVPRDNMVQR
                     AEINRKTVIDFDKTHPQADHYRNLARLIDKNEMFVIPKPLAIDQLEKLLIEYGVAS"
     gene            6740..7066
                     /locus_tag="VF531_03810"
     CDS             6740..7066
                     /locus_tag="VF531_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7713125.1"
                     /translation="MLMVKAIVRPEKCDGVMAALMAAGYPAVTKVSVYGRGKQRGIKI
                     GEVHYDELPKELLLMVVSDNDKEFVIKTIMESARTGDAGAYGDGKIFVIPVEEVYTIS
                     SGVKEA"
     gene            7112..7492
                     /locus_tag="VF531_03815"
     CDS             7112..7492
                     /locus_tag="VF531_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7713126.1"
                     /translation="MKEVMAIIRMNMMNQTKQALADAGITSFTATGRVAGRGKGKVDL
                     RTLQGAENGYDEAISQLSKGPRLYPKRLLTVVVPDTLVRTVVETLITTNRTGQPGDGK
                     IFVLPVSEAYRVRTGETGDLILDN"
     gene            7553..9184
                     /gene="nifD"
                     /locus_tag="VF531_03820"
     CDS             7553..9184
                     /gene="nifD"
                     /locus_tag="VF531_03820"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HEX7713127.1"
                     /translation="MQNKAGKPGSGEVKEDLLKIYPAKVARKRAKHIVPNDPDPEVMP
                     EVEANVRTIPGIITQRGCTYAGCKGVVLGPTRDILNITHGPVGCGFYSWLTRRNQTRP
                     QDDADANFMTYCFTTDLQDDNIVFGGTKKLRQAIEEACRIFKPKAIGIFATCPVGLIG
                     DDIHAVAREMKEKLGINVFAFSCEGYKGVSQSAGHHIANNQLFRHVVGLDDTPGQEPY
                     RINLLGEYNIGGDEFEIERILEKCGMKLVATFSGNSTYDRFANAHTASLNVIMCHRSI
                     NYVAEMMEERYGIPWIKVNFIGAESTAKSLRKIAAYFGTPELKARVEEVIRVEMPEVE
                     KVRAAIRPRCEGKLAMLFVGGSRAHHYQDLFREIGMKTVAAGYEFAHRDDYEGRRVLP
                     AIKIDADSRNIEQLAVTPDPARYRPRKSAAELEKMAAEGFRFKDYDGMMTEMDKDSLI
                     IDDISHYEMERMIKLYKPDIFCAGIKEKYAVQKMGVPCKQLHSYDYGGPYAGFKGAIN
                     FYQEIDRMVNTPIWKLIAAPWQKEPELIATLAVDE"
BASE COUNT         2609 a         1876 c         2334 g         2416 t
ORIGIN      
        1 aagccggaaa atttgaaaca actgtaaaag ataaatacaa ggattatttt aatgaggtca
       61 aaggcaataa ttccggggcc actctaattg gtttaagagg ttggggaaat gggccgacta
      121 caaaaccaga caatgaaaat cttaaaggtc aaaatacata tgatgatatg cttttagtat
      181 tgggtgaaga tggaaagtta ggggctttta gtgcggttaa ttttgagagc acggatgcta
      241 agggctggta taaatctaaa ggtgagaaga aaactttaac cggtgattat aacgatattg
      301 gtgatgtaac tgccgaggtt aattatgggt atcatggcga ttatcaagca ttatggctaa
      361 gaaataacta tcctgttaaa ggaaccaaac caaatggaga aatgacattt ttagaaggta
      421 tccatatcca taaaggcggg gatacttgga cattctcaaa agggtgcatt acaatttacg
      481 atggttccaa tccaaatttt tctggacaat gggatagatt tatgtcttat ttcaagataa
      541 actctcctat tattgatcct gatacaaatc aacctgtagt tgattctctt ggtagaacag
      601 aaggcgaaat aagtagacaa agagcaggaa gcatatcatt aatgagactt tagggggtga
      661 attgcatgag aaagatattt ttaatcataa cttttatttt atttacatta tcagttacaa
      721 caagtgctaa caatttggat attgataagt caattttaaa cataattaag agtataggga
      781 ttgatgcatc tgaagtagaa ctagaacttg aaccaaatga attaataagc tatgaagaat
      841 actcaaaaaa ggatttatat gaacaaaaga caaatgaagc agcttggagt acgatttggc
      901 atgacaatct agaaaagctt gatgtcttaa ccaaaggttc tttgactttc aagggatacc
      961 ttactaataa tttatttgat aataaagttg atacagcttg ggttgaagga gctaatggag
     1021 atggaatagg agaatggata tcaattgtat taaatgctaa gagaaccgac agaccgactg
     1081 ggatgttata ttttgggatg ataccgggat atttaaaaag cgataagatc tggaaggaaa
     1141 ataaccgagt taaaacggca cttctaatcg tcgaaagata taccggatat gatattcgca
     1201 caaaaagttt tcgttatgat tatcccataa ttcgtctaaa atttaaggat ttcaaagggt
     1261 tacaagtttt taatttactt gattatggag cacctcctac ttatgatcct aagagattat
     1321 gggttattat tgaaaatgtt tataaaggga ctaaatataa tgatacatgt atctcagaaa
     1381 tagttgtatc gggaacatgt gcacctgggg aggatgaggg ggaagaataa attaactggt
     1441 gccaaatcca taaaacaaaa ggctccaaaa ttagttggag cctttttgtg tttgattata
     1501 ttttttcatt cttggacttc gatatccgtt tctttaatta attgcatgag ttgaaggtgg
     1561 tcacggtgat tttcttcgat atgagcatca agtttggatt caagcttatc aactttttgt
     1621 tctaaattat tgaagcagtt attcatttca ctgaagcggt tattcatttc atctcgaagc
     1681 ccgtccaatc cctcgccaaa gacacggaat ttggctatta aatcctcaag caacactgct
     1741 accttgttat cttccatcca atcaccccgc aaaacagctt caaatttatt atatcataaa
     1801 caatttcatt ttgggacaag tttccaggaa agcgagtcag cgaatcaccc ctccggttag
     1861 ccaggaggtc gaagcaacat tgatgcgctt cccctctctc cgggcgagcc ggaaagaggg
     1921 gaagaatgcg ggatgagttg cattcgtcct ttttttgaaa gatgtatcac tacgttgagg
     1981 atttctcttg tatcttgatt accggaataa aacatattcc cctctttgcc gcctgtcggc
     2041 agagagggga atgctcagga ataacgatga tctcgaggcc atgtaagggg tgagtcgctt
     2101 ttaggccctt ctttgaggcc agagaaaact atatactgga ggaattctat gataacagat
     2161 agtattcttt atttattatt atctctttaa gcaacactta gccctggtga atggagtgat
     2221 cggcggcgac gccaagaagt tctactataa caacgatcac gaaggcaatg ggctggtggt
     2281 caccgatgag aatggaaaca aggtcgttga gcgggacttc acgccctttg gccaacgaat
     2341 cgaccaggcc ggacatgaag gcaagttccc ggaggagacg gaggacggat tcaccgggaa
     2401 ggattgggac gaggatattg ggctgtatta cttcaatgcc cggtggtacg acccggaggt
     2461 aggaaggttt atttccgaag attcggtggc ggatgatccg aatctgtata gctattgtgg
     2521 gcagaatccg gtaaataatg ttgatccgac agggaatttt tcgattggat tgcaacaggg
     2581 ttggaatatg gtgggttcta tgataaatgc tacggccttg ttgagtaaaa atgaaacttt
     2641 aggaatgatt tcatcggtat ttagtttatt tgttgccatt gacggtagat ggggattaag
     2701 taatatcatg gatgatatca ccgatttgtt cagcggaaac agttttgaaa aacaacttca
     2761 taaaaacatt agcggatata tagattcaaa gtttgaatta gaatctatag cccgtgatcc
     2821 cgatgaattg tatggagcta ttctaaatat taatagaagt aagtctgggt cggttgatag
     2881 tggtaaaaat ggtattgaaa gttctaccgc tactggttca atggatttcc agcaaaagaa
     2941 aataaagaag cgaaaataaa gaattataaa atcccataaa acaaaaggct ccaaaattag
     3001 ttggagcttt tttgtgtttg attatatttt ttcaatgaat cttcaatcgc ttcatctagc
     3061 agtcttgata ctggaattcg agtcttcttt gataattgtt gaaaattttc ccatagagct
     3121 ttatccacgg aacttgatat ctgaactcga tttttaagcc tcactgtagc cgtagcagca
     3181 tcaccttctt taagataata ctatgaagtt acttgaaaaa tcaaacaaat cgtgttatga
     3241 ttaatgtaac ttcatgaagc ttcattaatc acaaaataaa ggagcattgc gatgcgaaat
     3301 caccgatatg taactgtagg ctcggtccat cgtaataata aactcgtccc gttaattcgc
     3361 ctctccggta gttggttggc tgaaaatggc tttaaaaccg gccgcaaagt ccatatctat
     3421 gtaaagccgg gcagtttgac ggtgaatttg atcttgccgg aagatgagga ggaatatcga
     3481 attcaacctt tggaatagtg gaattcggac ttgtgaaggt aaaggaaatg caaaagtgaa
     3541 atgaatctgg ttatttaacc ctcttaattt caacctggac ttctttatcc aattctttaa
     3601 tcatttgcat aagttgttga tggtcccggc tattctccaa aaaacgctga tttgtttcat
     3661 ttcggaagtc agccatttct tgtctatctt cttttatttc atgatgaaga acatctaatc
     3721 cttccccgaa agctcgaaac tgtgcccgta aatcctcgag caacactgct accttgttat
     3781 cttccatcca aatcaccctg caaagcaact tcaaatttat tatatcataa accatttcat
     3841 tttgggacgg gtttccagga aagcgagtca gcgactcacc cctccagtta gcggtaggtt
     3901 gaagttacat agatgcgcct cccctctctc cgggcaggcc ggaaagaggg gatgaaaggg
     3961 agattagccg ttgtattttg aaggaacgcc gtaaaatgct gaaataaaac aaatccccct
     4021 ctttaccgct tggcggcaga gagggggaag ctcataacca acgataatct caaggttgag
     4081 agggggtgag tcgcctttag accctgctca tttcacccgt ttcaacttga cgacctcagt
     4141 atcaagttcg gcaatggctt gctgcaattg ctgatgttcc tgacggtttt gttctttaaa
     4201 ctcacggatc tcatctttaa tttctttaaa ccctcgatcc atcttttcgt ttagcatctg
     4261 caaactttcc ccgaaaaccc gaaactgtgc tcgtaagtcc tcaagcaaca ccgctacctt
     4321 gttatcttcc atccgtatca ccccgcaaaa caacttcaaa tctattatat cataaaccat
     4381 ttcattttgg gacaagtttc caggaaagcg agtcggcgac tcacccctcc agttggccgg
     4441 gaggtcgaag caacattgat gcgtttcccc tctcccaggt aagccggaaa gaggggagga
     4501 atgcgggatg attggtgttc ggtggttttg aaggaacgcg ccgagacccc actcatttca
     4561 cccgtttcag cttaacaacc tcggtatcga gttcagcaat ggcttgttgc agttgctgat
     4621 gttcctgacg gttctcgtcc ttaaaattgg ctaattcagt ctgtaatgag tcaatcttct
     4681 cattaacttc ttggaaccct ttatcaacct tactatctaa cagttgtaat ccttctccga
     4741 aaaccccaaa ctgcgctcgt aagtcctcaa gcaacaccgc taccttgtta tcttccatcc
     4801 gtatcacccc gcaaaacaac ttcaaatcta ttatatcata aaccatttta ttttgggaca
     4861 agtttccagg aaagcgagtc agcgactcac ccctccagtc ggtcgggagg ttgtagtcac
     4921 ataggtacgc cccctctctc cgggcaggcc ggaaagaggg gggagctcag aactaacgat
     4981 tatctcgggg ttgtgagagg gtgagtcgtt attaggcccg taaaatctct aattttttta
     5041 ttgttaactt catggaatat atcttgacgg agacaatttg aatatttaaa ctatctataa
     5101 tttaatattg tacgtttcat gcaaaagaaa ggagtgtttc tggatggact cggatgttat
     5161 gattcgtgag gcgacagacg ccgatattga ggcgatggtc cggttattgc gggtcttatt
     5221 caccatcgag gcggattttc atgtgaatga agccgcccag cgccgcggtc ttgccatgat
     5281 gttggaagat tcggatcacc gcagggtact ggtggcggaa gtggaaggga gtatttccgg
     5341 aatgtgcacc ggacaggctt tgatctccac cgccgaggga ggaatggcgg ttttggttga
     5401 ggacttggta gttctcgaag cgtatcgcgg ctacggtatc ggcagccgcc tgctggcggc
     5461 gatggaggcg tggggattcg gcatcggcgc caggcgcttg gagttgctgg ccgatcggga
     5521 aaaccggccg gcgttggatt tttaccagcg aaaaaactgg cgccagaccc ggctagtttg
     5581 cctgcataaa aaggaagcat aagagaaacg aaatattcaa gcgtaaaaac cgtcctccga
     5641 aaaaggacgg ttttttagtt ttgcaaaaag cttggatcgt gtatggaagg atacaaaaaa
     5701 caagatttct aaaaaattac taaattgtgc ttatgtgttt accagatgtt tactagatat
     5761 gtcatgttta tgtcagttta ccaattgaca atttgatatt tttccattat atttaactca
     5821 taactgacaa atatataaca ctgattccgg aaagtgagtg atgaggatgc gaaagattgc
     5881 catttatggt aagggcggga tcggcaaatc cactactacc cagaatactg tggccggact
     5941 ggcggagatg ggccggaagg tcatggtggt aggttgcgat cccaaggccg attccaccag
     6001 gttgttattg ggtggactgg cgcaacaatc ggtcctggat accttgaggg atgaagggga
     6061 tgatatcgac ctgggcgacg tcatgaagtc cggttttgca agcacgttgt gcgtcgagtc
     6121 cggagggccc gaacccggcg taggctgcgc cggacgggga atcatcacct ccatcaatct
     6181 cctggaacag ttgggcgcct atgaagagga taagaacctg gattacgttt tttatgacgt
     6241 tttgggtgat gtggtctgcg gcggtttcgc gatgccgatc cgtgagggaa aggcgcaaga
     6301 gatctatatc gtagtttccg gcgaaatgat ggcgatgtat gcggccaaca atatctgcaa
     6361 gggaatcgtc aaatttgccg agtccggcgg ggtccgcttg ggcggattga tctgcaacag
     6421 ccggaaggtg gacaacgagg aagccttgat tagagccttc gcggcacagc tcggaaccca
     6481 aatgatctat tttgttcccc gcgacaatat ggtccaaagg gccgagatca accggaaaac
     6541 cgtgattgat ttcgataaaa cccatcccca ggcggatcat taccgcaatc tggcccggtt
     6601 gatcgacaaa aatgagatgt ttgtaattcc caagccgctt gccatcgatc agctcgagaa
     6661 attgttgatc gaatacggtg tggccagttg acggcccttg gcgcggtagg gcagaaaaat
     6721 tattgaagag gtgatccgaa tgttgatggt taaggcgatt gtccggcctg aaaaatgcga
     6781 tggggtgatg gccgctttga tggccgcggg ttatccggca gtcaccaagg tctcggttta
     6841 tggccggggc aaacaacgcg ggattaaaat cggcgaagtc cattacgatg agcttccgaa
     6901 ggaactctta ctgatggtgg tttcggataa cgataaggaa ttcgtcatca aaaccatcat
     6961 ggagagcgca cgtaccggcg acgccggcgc atacggcgac gggaagatct tcgtcatccc
     7021 ggtcgaggag gtttacacca tcagttccgg agtgaaagag gcttgaggtt tgaccggagt
     7081 tatttcatca acctcaaagg gggaatcgaa gttgaaggaa gtcatggcga tcattcggat
     7141 gaacatgatg aatcagacga aacaagcctt ggcggatgcc ggaatcacat cctttaccgc
     7201 cacgggacgg gtggcgggac ggggcaaggg aaaagtggat ctacggacgc ttcagggtgc
     7261 cgagaatggc tatgacgaag ccatttccca attgagcaag gggccaagat tgtatcctaa
     7321 acggttgttg accgtggtcg ttccggacac attggtccgt acggtggtgg aaacgttgat
     7381 cacaaccaac cgtacgggac aaccgggcga cgggaagatc ttcgtcctgc cggtgtcgga
     7441 agcgtaccgg gtccggaccg gcgaaaccgg ggacctgatc ctggataact agcccgtgat
     7501 gatggttaac gtgcttacgc caggagaacg ggatgaaaag gaggatacct caatgcagaa
     7561 taaagcggga aaacccggtt cgggggaggt caaggaggat ctgctcaaga tctacccggc
     7621 caaagtagcc agaaaaaggg cgaaacatat cgtgcccaat gatcccgacc cggaagtcat
     7681 gccggaagtg gaggccaacg tccgcaccat accggggatc atcacgcagc gcggttgcac
     7741 ctatgccgga tgcaaaggag tcgtgctggg tccgacccgg gatattttaa atattactca
     7801 cggtccggtc ggatgcgggt tttacagttg gttgaccagg cgcaatcaaa ctcggccgca
     7861 ggacgatgcg gacgcgaact tcatgactta ttgtttcaca accgatctgc aagacgataa
     7921 tatcgttttc ggcggcacca aaaagctgcg tcaggcaatt gaagaagcat gccggatttt
     7981 taaacctaaa gccatcggca tctttgccac ttgcccggtg gggttaatcg gcgatgacat
     8041 tcatgcggta gccagggaga tgaaagagaa actgggcatc aatgtctttg ccttcagttg
     8101 cgaaggatac aagggagtca gccagtccgc cgggcatcat atcgccaata atcaattatt
     8161 ccggcatgtg gtgggacttg acgatactcc ggggcaggag ccttaccgca tcaatctcct
     8221 gggcgaatac aacatcggcg gcgacgagtt cgaaattgaa cggatcctgg aaaagtgcgg
     8281 catgaaactg gtggccacct tcagcggtaa ttccacctac gatcggtttg ccaatgccca
     8341 taccgccagt ttgaatgtaa tcatgtgtca ccgttccatc aattatgtgg cggagatgat
     8401 ggaagaacgg tacggtatcc cgtggattaa ggtcaatttc atcggggccg agagcaccgc
     8461 caaatcgttg cggaagatcg ccgcctattt tgggaccccg gagttgaaag cccgggtgga
     8521 agaggtgata agagtggaga tgccggaggt tgaaaaggtg cgtgccgcaa tccgtccgcg
     8581 ttgcgagggg aagctggcga tgctttttgt cgggggctcc cgggcccatc attatcagga
     8641 tttattccgg gagattggca tgaaaaccgt ggctgcggga tatgaattcg cccaccgtga
     8701 cgattacgaa ggccggcggg tactgccggc aattaagatc gacgccgaca gccggaatat
     8761 cgagcagctc gcggtcaccc cggatccggc gcgttatcgc ccgcgaaaga gtgcagccga
     8821 actggagaag atggcggcgg aaggattccg gttcaaagat tatgacggaa tgatgacgga
     8881 gatggataag gacagtttaa tcatcgatga tatcagtcat tatgagatgg aacggatgat
     8941 caaactttac aagccggata ttttctgtgc gggcattaag gagaaatacg ccgtccagaa
     9001 aatgggcgtt ccctgcaagc aattgcacag ttacgattat ggcgggcctt atgccgggtt
     9061 caagggagcc atcaatttct atcaggagat cgaccggatg gtcaatacgc cgatctggaa
     9121 attgatcgcc gccccgtggc aaaaagaacc ggaacttatc gcaactttgg ccgtagatga
     9181 ataaagaacc cggtttatac gtgcataagc ggaccggcta gaaatgagtt tcagg
//
LOCUS       DASVMB010000118         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_14712,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000118 DASVMB010000000
VERSION     DASVMB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14712"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3189)
                     /locus_tag="VF531_03825"
     CDS             complement(<1..3189)
                     /locus_tag="VF531_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7713128.1"
                     /translation="MKVTVGYGELKEHRLILERLKTALARQEFGLIVIFPTLSLLEEI
                     QDELLADPEVLGCGGFRLLLFEGFIQELGQELGEGRPKPSPLEQELLLYECFKRLDAA
                     GKIDYLNRVPCNSGYRQAILQGFAEWKRSGLTAERFRNWATGRGPRVEQLALLYDRYQ
                     QALVERGFGDEDLILAKLEKRSGVTGNRGSQETVLLYGFSDLTPLQMRFIQALSSRFR
                     FEAMIDPTLVAEFQDLIAKHFLFVIPTRSGSGATGSALKKLQQRFWAGAPVPEPIEPE
                     DLSLQIWQAAGATGRATGIAREIRDWLRSHPDWGLHDFLILTPTPQAFIQTARPVFQE
                     YGLTLAEMALPLQQSPAITRFLEALRVVNADWQWNEIAVLIRRSYPKQLNAIVDRLLL
                     EIANRYGAVSGKRRWLNLIRQEAFQQYFCELGLDLGSLTAILRKTAAIPDSATLPEYL
                     TFCRDWFNVPPVPLKPDDPLLTLEIQDGRAVRELVRLIEIWIRYLESTVDGPSAVKDA
                     ILRCSEFQRQFEDFLVRTELTPLSPLTAELRVLPLRESRGLKARVVFIVGLEQGVLPR
                     FYVNDWKLTVADRLDFRSRGVELETGVQYQIQERLAFYWALQTAVQSLYLVCQNQDDN
                     GQPLVRSTFLEEIGDYFPELEKRCRILPLVSKSPSEWNDCYTAYEKRSFWVERLNAPA
                     QEIAGDNATAGNLFGAGEAVNTPKAPNTSGAINEYSKMAQWMEPSCRILAAELLNWRD
                     RRSPQSGFFHDQEPLERLHRIFGSGHVWSITALEDFRNCPYRFFLKHVLKVESLVEPE
                     LFPTGLELGNLYHQILQDFCEQYRGLSLAAEKTAEYRRVLAGCLANFYREWQESAPND
                     LLKLVLLIQEKQIWRTLNLWLDSEIRWAEATNHRFRIFGLEYAFGLAKGDYDPGSLSE
                     PYQLETTDGPIRFQGKIDRVDQDESGYFTVYDYKSGRGPSTQEVLNANRLQLAVYLLA
                     LEQLSFGPDRALGGSYIGLRQPSRSRGGVWRADRIALPFSGKAALAGETWEQWLRGVE
                     TVLVETVTAIRGGEFGSLGADCPDYCEYRDICR"
     gene            complement(3626..>4377)
                     /locus_tag="VF531_03830"
     CDS             complement(3626..>4377)
                     /locus_tag="VF531_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7713129.1"
                     /translation="FGFGIGAVLVLAAAGRVQLHASNKGLLIARGVFGGGGILLYFIA
                     LAGGSLTNSTVLNNTYPIFATLAAAVTLKERIGWGTALSLVLSWIGVGMLIHPDFHHL
                     FWPDLLALISGVLGGVAIVVIRELRRNNESAWTVFFYLSIFGFLASLAFALPVWVWPD
                     EASIWYLLLTAVFGLIAQVTMTASYKYCRTAIGSILSMTTMVFTALIGLTFLGEKLTM
                     LEAAGTALIVLGCAVAVWLAEAKPKEKICEG"
BASE COUNT         1125 a         1220 c         1079 g          953 t
ORIGIN      
        1 gcggcagata tcacggtatt cgcaataatc gggacaatcg gcgccgaggc tgccaaactc
       61 cccgccccgg atcgcagtga cagtctccac cagcaccgtc tctacccctc gcaaccattg
      121 ttcccaagtt tcaccggcca gtgctgcctt gccgctaaaa ggcagggcga tacgatcggc
      181 ccgccaaacc ccgccccgac tccgggaggg ttgcctgagt ccaatataac tcccgcccaa
      241 agcccgatcc gggccaaagg ataactgttc aagcgcaagg agatataccg caagttgcag
      301 gcggtttgca tttaaaactt cctgggtgga tggacccctg cccgatttat aatcataaac
      361 cgtgaaatac ccggattcat cttgatctac ccggtcgatc ttgccctgga accgaatcgg
      421 cccatcggtc gtctccaact gatatggttc ggataaagaa ccgggatcgt aatccccttt
      481 ggccaaaccg aaggcatatt ccagcccgaa gatccggaaa cggtggttgg tagcctcggc
      541 ccaacgtatt tcggaatcaa gccagagatt gagggtgcgc caaatttgtt tctcctggat
      601 aagcaggacc aatttgagca ggtcattcgg agccgattcc tgccattccc gatagaaatt
      661 cgcgagacaa ccggccagaa cccgacgata ttcggcggtt ttctccgcgg ccagtgacag
      721 gccacggtat tgttcgcaaa aatcttgcag gatttgatga tagagatttc ccaattccag
      781 accggttgga aataattccg gttcgaccaa ggattccact tttagcacat gttttagaaa
      841 gaaccggtaa ggacaatttc tgaagtcctc cagtgcggta atcgaccaga catgtcccga
      901 tccgaaaatc cggtgtaacc gttccaaagg ctcctgatca tggaaaaagc cggattgagg
      961 actgcggcgg tctcgccaat tgagaagctc tgccgccaga atccggcagg aaggctccat
     1021 ccattgcgcc attttggaat attcgtttat agcgccggat gtgtttggtg cttttggtgt
     1081 gtttactgct tcacccgcgc caaacaagtt gccggcggtt gcattatctc ccgcgatttc
     1141 ctgagcagga gcattcaatc gctcaaccca aaaagatcgt ttctcgtagg cggtgtaaca
     1201 gtcgttccat tccgaaggag acttggacac cagcggtaaa atccggcaac gcttttcaag
     1261 ttcgggaaag taatcgccga tctcctccaa aaaggtggag cgaaccaggg gttgaccatt
     1321 gtcgtcctgg ttttggcaga ccagatagag ggattggact gcggtttgca acgcccaata
     1381 aaacgcgagc cgttcctgaa tctgatactg aaccccggtt tccagttcca cgccccggct
     1441 gcggaagtcc aaacggtcgg cgacagtcag cttccaatca tttacataaa accggggcaa
     1501 gaccccctgt tccaatccga caatgaagac tacccgggcc ttaagccccc tggactcccg
     1561 gagcggcagt acccgaagct ctgctgtcaa gggtgaaagg ggtgtaagct cggtccggac
     1621 taaaaaatct tcaaattgac gctgaaattc actgcaacga aggatggcat ccttgacagc
     1681 ggatggccca tcgacagttg actccagata gcggatccaa atctcaatca accgtaccaa
     1741 ttcgcgaacg gcccgcccgt cttggatctc cagggttaaa agcggatcat ccggcttaag
     1801 cgggaccggg gggacattaa accagtcacg gcagaaagtc agatactcag gtagagtagc
     1861 cgaatccgga atggcggcag ttttgcgcaa aatagcggtc aaagacccca gatcgagtcc
     1921 caattcgcag aagtattgct ggaaggcctc ctgacgaatg agattaagcc agcgacgttt
     1981 cccggagaca gcgccgtagc ggttcgcgat ctccagcagc aaccgatcta cgattgcgtt
     2041 aagctgtttc gggtaagaac gccggataag caccgcgatc tcgttccact gccaatccgc
     2101 attcaccacc cgcagcgctt ccagaaaccg ggtgatcgcg ggcgattgtt gtaaaggtaa
     2161 tgccatttca gccagagtga gaccatactc ttgaaaaacc ggcctggccg tttggatgaa
     2221 cgcctggggt gtgggagtca ggatcagaaa atcatgtaaa ccccaatcgg gatgggagcg
     2281 taaccaatct ctgatttccc gggcgatccc tgttgcccgt cccgtcgccc cggccgcctg
     2341 ccaaatttgt aatgacaagt cctccggttc aataggctca ggcacgggag ccccggccca
     2401 aaatctctgt tgcagctttt ttagggcgct gcctgttgcc ccagacccgg accgggtagg
     2461 gatgacaaac agaaaatgtt tggcgatcag atcttgaaac tctgctacca aggtcgggtc
     2521 gatcatcgct tcgaaccgga accgggagct gagggcttga ataaaccgca tttgcagcgg
     2581 agtcaggtcg ctgaacccat ataaaagaac cgtttcctgg gaccctctat ttccagtcac
     2641 cccggatctt ttctccaact tcgcaaggat taaatcctca tcaccaaaac cccgttccac
     2701 caatgcctgc tggtaccggt cataaagcaa agctagttgt tccacccgcg ggccccggcc
     2761 ggtcgcccag ttccggaaac gttccgcggt aaggccgctc cgtttccatt cggcgaatcc
     2821 ctgtaaaatc gcttggcgat atccggagtt acacggcacc cggttaagat agtctatttt
     2881 tccggccgca tccagccgct taaaacattc atagagtaaa agttcctgtt caagcgggct
     2941 gggcttaggg cgcccctcac ccaactcctg gccgagctct tgaatgaacc cttcaaaaag
     3001 caggagccgg aagccaccgc atcccaggac ttccggatcc gccagcaatt catcttgaat
     3061 ctcttctagc aggctcaagg taggaaagat tacgattaaa ccgaattctt gccgggcgag
     3121 ggctgtttta agccgctcca ggatcagacg gtgctctttc aattcgccgt aaccgacggt
     3181 aaccttcata atagcagccc tccattccgt aaataggcaa taggcaacag gcactagata
     3241 aaagacccta ggcgtaggta tgagtaacgc actcaaaggg gcggcaatag acaacaggga
     3301 ataggccaca gaaatgcgaa agaagagagt gtgtgtaagg gatacccttg attccccgat
     3361 attagttctt taaatttcat atcccaaata cacaccagaa ttagttctcc tgttgcctat
     3421 tgcctatgga agaattcttg atttcccccc ccgattcctg ccggacaggt tgcgaaggaa
     3481 cttcgcttgc ggtataatgg aaacataacg gaggtgattg ccatgaaaaa tggctatttc
     3541 ctttcgttcc gggtctggtg acccgatatt ataaatggga gccccgcgag ggagctccct
     3601 tatttatttc cctgttatag gtttgtcatc cttcacatat tttttcctta ggctttgcct
     3661 ccgccagcca aaccgccacg gcgcatccca atacgattaa ggccgtccct gctgcttcga
     3721 gcattgtcaa tttttctcct aaaaatgtca agccgattag ggccgtaaaa accatggtcg
     3781 tcatcgaaag gatactgcca atcgcggtcc ggcagtattt ataagaggcg gtcatcgtta
     3841 cctgggcgat tagcccaaat acagccgtca ataaaagata ccaaatactt gcttcatccg
     3901 gccagaccca gacaggcagg gcaaaagcca gggaagctaa aaaaccgaaa atactgaggt
     3961 aaaaaaagac cgtccaggcg gattcattat tgcggcgtaa ttcccggatt acgacaatcg
     4021 ccacccctcc cagaactccc gagattaaag ccagcagatc cggccagaac aaatggtgaa
     4081 aatccggatg gatcaacatt cccaccccga tccaggataa taccagactc aaagcggttc
     4141 cccagccgat tctttctttg agagtcacgg ccgcggccag cgtcgcgaag atcgggtagg
     4201 tgttattcaa aaccgtactg ttggtgaggg atccaccggc tagggcgatg aaataaagta
     4261 gtatcccgcc acccccgaag accccccggg caatcagtaa acccttattt gaagcatgta
     4321 attgaacacg tccggcagct gccaaaacca gcacagctcc gattccaaag ccaaaac
//
LOCUS       DASVMB010000119         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_678552,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000119 DASVMB010000000
VERSION     DASVMB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678552"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1550)
                     /locus_tag="VF531_03835"
     CDS             complement(141..1550)
                     /locus_tag="VF531_03835"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX7713130.1"
                     /translation="MPIGLQFIVAVCGGVFIILISALFLWRPGINIWTDAFVKRLMKD
                     PYPENIAEMYNVFTKVGFQNVLESDLRSTFGEPLKRPFGSPKHLSPWDKLLFNPVYFT
                     RKPTVESVAIDTKVTIGPQAKRPLEIDIPIMIAGMGWGSGLSLNAKIALAKGADLVKT
                     ATGTGAGPFLPEERKHVKRLIIQYHRGFWAKEAEVLRQADAIEINLGYGAHESAPVIW
                     KYKDLSPEFRDYMKLKPGEDLIEEATLPNARTATELAKLVQYLRKLTNGVPIGVKLGA
                     THYLEQELDFLAEAGIDFISIAGGEAGIYYGPGILADDVGLPTLPALCRTASYLKQRG
                     LQNKVSLIVSGGLVTPGHFLKALALGADAVAIGTVAVLAMTHTQMTKVFPWEPLSELV
                     FENGKSKNKLFIDKAAMNVANFLKSCNEEIILAIRCMGWSKLKELSTADLCSTSAEIA
                     SMTGVDHCLYPPKGYGIKQ"
     gene            complement(1679..2272)
                     /locus_tag="VF531_03840"
     CDS             complement(1679..2272)
                     /locus_tag="VF531_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018393135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HEX7713131.1"
                     /translation="MTNSRMNPKVDEYLRKAQKWREEFEKLRMIILDCQLTEELKWGK
                     PCYTFQGSNIVLIQGFKEYCALLFFKGALLKDPHGILIQQTENVQAGRQIRFTNVREI
                     VTMETILQAYIDDAIEVEKAGLEINFKKNTGLTIPEEFQNKFEAIPALKTAFEALTPG
                     RQRAYILYFSEPKQSKTRESRVEKCMQQILNGKGLND"
BASE COUNT          616 a          595 c          537 g          709 t
ORIGIN      
        1 cagggtttct agtgagcccg gatttttaat caaaccggca atgctttggc gaaatttggc
       61 cataaaaaca aagattaagg ccaacgaggc caaacccgcg cccaataaaa ctccggtaat
      121 tggaatccaa aatttcaaag tcattgtttg attccatagc ctttcggggg ataaagacaa
      181 tgatcgactc cggtcatgga ggcgatctca gcggatgtgg agcaaagatc ggcggttgac
      241 aactctttta gtttgctcca gcccatgcag cggatggcta atatgatctc ctcattacaa
      301 ctcttaagaa aattcgcgac attcattgcc gctttatcga tgaataattt gttctttgat
      361 ttgccatttt caaaaaccaa ctccgacaag ggttcccaag gaaaaacctt ggtcatttga
      421 gtatgcgtca tggccagtac ggcaacggta ccgatggcta cggcatcggc acccagagcc
      481 aacgccttga gaaaatgtcc cggagtcacc aatcctcccg agacgatgag ggaaacctta
      541 ttttgtaaac cacgctgttt gaggtagctg gccgtccggc acagcgccgg cagggtggga
      601 agaccgacat catcggcaag tattcccggg ccgtaataaa ttccggcttc tcctccggcg
      661 atgcttataa agtcgatccc tgcttcggcc aagaagtcca gttcctgctc cagatagtgg
      721 gtagctccga gtttgactcc gatgggcact ccgttggtta acttgcggag atactgcacc
      781 aatttggcta attcggttgc ggttctcgca ttggggagtg ttgcctcttc gattaagtcc
      841 tcgcctggtt ttagcttcat gtaatcgcgg aattccgggc ttaagtcctt atacttccat
      901 atcaccggcg ccgactcatg agctccatat ccgagattga tttcaatcgc atcggcttgc
      961 cgcaaaacct ctgcttcttt tgcccaaaag ccgcgatggt attgaattat cagtcgtttg
     1021 acatgcttac gttcctcggg taagaaaggg ccggctccgg tgccggtggc ggtttttacc
     1081 aaatcggcgc ctttcgccaa ggcaattttg gcgtttaggg ataatccgct gccccaaccc
     1141 attcccgcaa tcattatcgg gatatcgatc tcaagcggtc ttttggcttg tggtccgatg
     1201 gttaccttgg tatcgatagc caccgattcg accgtcggtt ttctggtaaa atacaccgga
     1261 ttgaaaagca atttatccca gggagataag tgttttgggc ttccgaaggg gcgtttgaga
     1321 ggttcaccga atgtcgagcg caaatcactt tccaagacat tttggaatcc gactttcgtg
     1381 aagacgttat acatctctgc gatgttttcc ggatagggat ctttcatcaa ccgctttaca
     1441 aaggcatcgg tccagatgtt gataccaggc cgccaaagga agagggcgga aatcaggatg
     1501 atgaaaactc ctccacatac tgcaacgata aactgtaacc cgatcggcat ggtttttccc
     1561 ctatctgaaa cttcaaaacg ctggtctctt gaactagctt tgccattgaa ggtaaaatat
     1621 atgaggatca tatccgaccc caggcgaacg gcatggacga atcctcacat cgaatacact
     1681 aatcatttaa tccctttcca ttgagaattt gctgcataca tttttcaacc cttgactctc
     1741 gggttttgga ttgtttgggt tcagaaaaat aaagaatgta tgctctttgc cgtcccggcg
     1801 tcaatgcttc aaaagcagtt ttcaaggcgg ggattgcttc gaatttattt tgaaattcct
     1861 caggaattgt gagtcccgta ttctttttaa aatttatttc caaaccggct ttttcaactt
     1921 caatggcgtc atcaatataa gcttgcaaga tggtttccat tgtaactatt tctcgaacat
     1981 tggtgaaccg gatctggcgc cccgcctgta cattctccgt ttgttggatt agaatgccat
     2041 ggggatcttt taacaaggcg cctttgaaaa acagcagtgc acagtattct ttaaatccct
     2101 gtattaaaac tatattactt ccctgaaatg tgtaacaagg tttaccccac ttcaattctt
     2161 cggtcagctg acagtcaagt atgatcattc tcaatttctc aaattcttcc cgccactttt
     2221 gggctttcct taaatattca tcaacctttg gattcattct actatttgtc atcaaggaac
     2281 cccctcttca ttttttcgat cacggggata attgccactc ttttaaagat attttctcaa
     2341 taagaatacc cttccaccgc cacggcatgc gttttcgatc tacccctgaa cgatttttga
     2401 gctattttct ttctggccgg aaactctaga aagaatgaat acagggcgtc gtcatgg
//
LOCUS       DASVMB010000120         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_237651,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000120 DASVMB010000000
VERSION     DASVMB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237651"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VF531_03845"
     CDS             complement(<1..595)
                     /locus_tag="VF531_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7713132.1"
                     /translation="MRYRQIQGTDLKPATICMGGLPFITGGTPNPFSLLDAYTELGGN
                     FIDTANVYGKWLPDKINSSEFTIGRWMKERRNRSTTIIATKGGHPDLATMQISRLSRP
                     EVAADLEESLKALQTDWIDLYWLHRDDETLPVAEILGYLNEFAAEGKIRYFGCSNWKP
                     NRIREALNYAKSHQLKGFVGNQAMWSLASVSPNGLTDP"
     gene            complement(637..2313)
                     /locus_tag="VF531_03850"
     CDS             complement(637..2313)
                     /locus_tag="VF531_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713133.1"
                     /translation="MSNKTAFEVRGIETHSRHMWEWEHVKRMIDFARENDLNALVFHE
                     NDLIDQVVYAERFISRAMMKRKFPVYQYGTDNNCDYIRKVAQTAKEQGIDFYLEVKEL
                     WFRTYVLFSYPELLHNGIPCPYAPFWWDFLQAKLETLYQKIPEVSGIIVSIATKESRL
                     SIINSRCHCEKCRHTSADQWFQKIIDTMYQPTKAAGKKLIIRDFAWSPKDLDDVVSAV
                     ENSPSDVVISLKATPHDYYPNFPDNPRIGNVGNHPQWIEYDVWGQFYGWGVFPCNVLE
                     DIKKRMEYDLSKGATGFIARTDWELVSEGTAFDGLNKLNLYGMAKLSRNIHADFDDIY
                     HEWLTHPVSTAFSESDVPIYPGSGKDQHKLNIAKIRAILEQTCDVMVHGTYVNGHVFN
                     FASAFPEDLDTAWFMMEENHSLADWDASKKSGLDLNPDNIQTILAEKELALEKIKILY
                     QQLQNDGNAMGLNPIFYKDLLKTFEMYVIYIEGFLHTTRACFMTKYYMADQNEILKKK
                     AREAIASLAAYAERLKRLVYRTEVHHYVYMLLDHLRLESLISDLNSKLDS"
     gene            complement(2362..>2794)
                     /locus_tag="VF531_03855"
     CDS             complement(2362..>2794)
                     /locus_tag="VF531_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713134.1"
                     /translation="LKWINTFQGAIIPTISQAFGVFLMRQYCLTIPTELIEASRIDGC
                     SDFRTFWQIIFPLCKPAMAVNIIFQFMWRWNDLLWPIVIIRDQNMYTIQQGLAFFRED
                     PSVAGGPIMAMSIVSVLPIVAIFFALQKYFVQGIAMTGLKS"
BASE COUNT          735 a          688 c          636 g          735 t
ORIGIN      
        1 tgggatccgt aagaccattt ggactgaccg aagccagact ccacatggct tgattaccga
       61 caaacccctt gagttggtgg cttttggcgt agtttagcgc ctccctgatc cggttgggct
      121 tccaattgga acacccgaaa taacgaatct ttccctcagc cgcaaattca tttaagtacc
      181 cgaggatctc cgccaccggg agagtttcgt catcccgatg caaccaatag agatcaatcc
      241 aatcggtttg gagcgccttg agactctcct ccaggtcggc ggctacttcc ggccgggata
      301 ggcgggaaat ctgcattgtt gcaagatcgg gatgcccgcc tttcgtggcg ataattgttg
      361 tagagcgatt ccggcgctct ttcatccagc gcccaatagt gaattcgctg ctattaatct
      421 tgtcgggcag ccattttccg tatacattag cggtatcaat aaaattaccg ccaagttcgg
      481 tataggcatc taacagactg aacgggtttg gggttccccc cgtgatgaag ggtaatccgc
      541 ccatacaaat cgttgccggt tttaagtctg tgccctgaat ctgacgatac ctcatgttaa
      601 ggcacctcca tccaatcaat catcggagtg caaagattac gaatctaact tggagtttaa
      661 atcgctgatt aaactctcaa gtctcagatg atccaataac atataaacat aatgatgtac
      721 ctcggtccgg taaaccaggc gtttcaagcg ttcagcgtag gcagccagac ttgcaatagc
      781 ctctcgggct ttctttttaa ggatttcatt ctgatccgcc atataatact tggtcatgaa
      841 gcaagcccgg gtagtatgga gaaaaccttc aatatagatc acatacattt caaaggtttt
      901 gagaaggtcc ttataaaata tggggttcaa acccatggcg ttgccatcgt tttgtaactg
      961 ttgataaaga attttgatct tttccagggc caattccttc tcggccaaaa tcgtttggat
     1021 gttatcgggg ttcagatcca gaccggattt cttggaggca tcccaatccg ccagactatg
     1081 gttctcttcc atcatgaacc aggccgtatc caaatcttcc gggaaggcgc tggcaaaatt
     1141 gaaaacatga ccgttcacat aggtgccatg caccatcacg tcacaggtct gctcaagaat
     1201 ggcacgaatt ttggcgatat tcaatttatg ttgatctttt cctgacccgg gataaatcgg
     1261 aacatcgctt tcggaaaacg cggtcgaaac cgggtgggtc aaccattcgt gatagatatc
     1321 gtcaaaatca gcgtgaatgt tacgactcaa tttggccatc ccgtataggt tcagtttgtt
     1381 caatccatcg aaggcggttc cctcactgac cagctcccag tcggtccggg cgatgaaacc
     1441 ggtagctccc ttgctcagat catattccat acgcttttta atgtcttcga gcacattgca
     1501 gggaaagaca ccccagccgt aaaactggcc ccaaacgtcg tactctatcc actgcggatg
     1561 gtttcctaca ttaccgatcc gcggattatc cggaaaattt gggtaataat catggggagt
     1621 cgctttcagg ctaatgacta catctgaagg ggaattttcg accgcagata ccacatcatc
     1681 caaatctttc ggtgaccaag caaaatcacg aatgattaat tttttcccgg cggctttcgt
     1741 aggctggtac attgtatcaa taatcttttg aaaccactga tctgccgagg tgtggcggca
     1801 tttctcacaa tgacaccggc tgttgatgat cgaaagcctg ctctctttag tggcaatgct
     1861 gacgatgatc cctgagactt cgggtatttt ctgatataaa gtctccagct tggcctgaag
     1921 aaaatcccac cagaaaggag catagggaca aggaatcccg ttatgcaaaa gctccgggta
     1981 actgaagaga acataggtcc ggaaccaaag ctccttaacc tccagataaa aatcgatccc
     2041 ctgttctttg gcggtttgag ccaccttccg gatataatca caattattat cggtgccgta
     2101 ttgatatacg ggaaatttcc gtttcatcat tgcccgggat ataaaacgtt ctgcatagac
     2161 cacctggtca ataaggtcgt tctcatgaaa gaccaaggca tttaagtcgt tttccctggc
     2221 aaaatcgatc atccgcttaa catgttccca ctcccacata tgacggctgt gagtctcgat
     2281 acccctgact tcaaaggcag ttttgtttga cataaccatt cctccctaga tcgctttcat
     2341 catatcaacg tttaggcggt gtcatgattt gagacccgtc atggcaatac cttggacaaa
     2401 atacttttgc aaggcgaaga agatcgcgac aattggcaat acggacacga tcgacatcgc
     2461 cataatgggt cctccggcca cggaggggtc ctccctgaaa aaggccaaac cctgttggat
     2521 ggtatacatg ttttggtcgc ggataatgac gataggccat agaagatcgt tccagcgcca
     2581 catgaactga aagatgatgt taaccgccat cgccggtttg caaaggggaa agatgatctg
     2641 ccagaatgtc cggaaatcgg agcatccgtc aatccgtgag gcctcaatga gctcggtcgg
     2701 tatggtgaga caatattggc gcattaaaaa caccccgaat gcctggctga tggtaggaat
     2761 aatcgctccc tggaaggtat tgatccattt caac
//
LOCUS       DASVMB010000121         5122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_678590,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000121 DASVMB010000000
VERSION     DASVMB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678590"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VF531_03860"
     CDS             <1..1277
                     /locus_tag="VF531_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX7713135.1"
                     /translation="NTRLGGDDFDERVVNWMVEQFKKENGVDLKNDRMALQRLKEAAE
                     KAKIELSGLTSTGINLPFITAVNGEPKHLDLTLTRAKFEELTADLVQATAGPTQRALA
                     DAGLEAKDLDKVILVGGSTRIPAVQEIVKKITGKEPYKGVNPDEVVAVGAAIQAGVLT
                     GEVKDIVLLDVTPLSLGLETLGGVFTRIIDRNTTIPTSKSQVFSTADDNQTAVDIHIL
                     QGERPMATDNITLGRFRLEGIPPAPRGIPQIEVTFDIDANGIVHVSAKDKGTGREQKV
                     TITTSGGLKEDQINKMMKEAEAHAEDDKRKREEADAVNEADSLIYTTDKTLKDIGDKA
                     DKSHVEKIQKAKEELQKAVTAKDIPEIKTKKEALEKALHELSATIYNQSAGQAGAQGA
                     PGAGQEGPTGYGPQNEGPTVDADYKVQDDKDK"
     gene            1460..2596
                     /gene="dnaJ"
                     /locus_tag="VF531_03865"
     CDS             1460..2596
                     /gene="dnaJ"
                     /locus_tag="VF531_03865"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7713136.1"
                     /translation="MAKRDYYEVLGVGREASEEEIKKAFRQLARKYHPDVNKDNPKEA
                     EAKFKEVNEAYSVLSDPQKRAAYDQYGHAANDPNFAGGQGFGGVGGDFSFDGFGDIFD
                     MFFGGGGGGRHKTGPQRGNDLRFDLEISFEEAAFGKETTVEIPRTEICPNCNGNCAKP
                     GTPIKTCPACNGTGQVQVTQNTAFGRFVNVRTCERCHGAGKTIETPCPECGGSGRVRK
                     NRKIDIKIPAGVEGGSRLRVAGEGEAGLRGGSPGDLYVYIYVKPHPKFERQGNDVISE
                     IKLSLTQAALGTTVMVDTLDGRVELKIPEGTQHGTSFRMRGHGIPHLRGHGRGDHHVR
                     VKVTVPTKLSGEQRELLQKLAHSFGENVDDDDKGFMGKVRDAFK"
     gene            2918..3859
                     /gene="prmA"
                     /locus_tag="VF531_03870"
     CDS             2918..3859
                     /gene="prmA"
                     /locus_tag="VF531_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7713137.1"
                     /translation="MSGSYWSEIKLEVPKEWGELVAEIFQEEGAGGVIYEDPEILSER
                     PFQDDEYLGDEAQALIPRRFGLKVYFPVDDRLGERIERVKQRIGQILESVPEFELRQV
                     QEEDWAEAWKTYFKPEHIGSVVIKPSWESYQPVSGEIVVELDPGMAFGTGTHPTTRLC
                     LSLLQEQAPGRNNMLDVGTGSGILAVTAAKLGVGRIVASDIDPLAVKVATENVQRNGV
                     CDQVRVVEGDLLRLKLNTPFELVVANIITNAILKLLPDLSGVLAPNGRFLASGIIDER
                     FPEVEAALRGLGFMLVKTVTAEGWTALVAQRSSSTIS"
     gene            3904..4662
                     /locus_tag="VF531_03875"
     CDS             3904..4662
                     /locus_tag="VF531_03875"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX7713138.1"
                     /translation="MTRFFIPSSQIEGNRVFLRGADRHHLLTVLREGPGAEIMVLNGR
                     GEEFQAKITAISPDAVEAEICRRSERVVEPRLRINLAQSLPKADKFEWIIQKNTEVGV
                     ARFQPVLSERSNIRLDRDTTAKKMERWRRIIQEAAEQSGRAVLPELEPIRSWDRFLEE
                     LRPGLILIPWEGEQTRSLREILSRKQPADGAAAWPGEVTVIIGPEGGFSLDEVAEAQS
                     RGAIPVTLGPRILRTETAGLVVAAALLYHFDDLG"
     gene            complement(4665..>5122)
                     /locus_tag="VF531_03880"
     CDS             complement(4665..>5122)
                     /locus_tag="VF531_03880"
                     /inference="COORDINATES: protein motif:HMM:NF022530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqhG family protein"
                     /protein_id="HEX7713139.1"
                     /translation="GSGLLWELTSRKPLREPPRRPIKRNISYRDAFEALFNHLKWLLQ
                     NHDRKWLESAKTRWEEELRYLEAYYRENRTETPDADHSGFYQRAAEIYRKFQPAIRIQ
                     ICNISLCYLPLVNYTVEPFDLPGTRQLLIYDPLRRKLRQQKTSPAQEVN"
BASE COUNT         1276 a         1193 c         1525 g         1128 t
ORIGIN      
        1 gtaacactcg cctgggcggt gacgatttcg acgaacgggt cgttaattgg atggtggaac
       61 agtttaaaaa agagaatggc gttgatctca aaaacgaccg catggccctg caacgtttga
      121 aggaagcggc ggagaaagcc aagatcgaac tctccggcct cacctcaacc ggaatcaacc
      181 ttccgtttat tacagcggtg aacggtgagc cgaaacacct tgacctgaca ctgacccggg
      241 ctaagtttga agaacttact gcagacttgg ttcaggctac tgctggcccg acccaacggg
      301 ctttagccga tgccggcctg gaagcgaagg atctcgataa ggtgatcctg gtaggaggtt
      361 ccacccggat cccggcagtt caggaaattg tcaagaagat aaccggcaag gagccttata
      421 aaggggttaa ccccgacgag gtagtggcgg tcggcgcggc gattcaagcg ggtgttctga
      481 ccggtgaggt caaggatatc gtactcttag atgtaactcc gctttcgttg ggccttgaaa
      541 ccctgggcgg tgttttcacc cggattatcg accggaatac aaccattccc acttcgaaaa
      601 gccaagtctt ttcaacagcc gatgataatc aaacggcggt ggatattcat attctacaag
      661 gggaacggcc gatggccacg gacaatatca cgctgggccg gttccggttg gaagggatcc
      721 cgccggcacc ccgggggatt ccgcagatcg aagttacctt cgatatcgat gccaacggta
      781 tcgtccatgt ctccgcgaaa gataagggta ccggcaggga gcaaaaggtg acaatcacca
      841 cttccggcgg gttgaaggaa gaccagatca acaagatgat gaaggaagcc gaagcccatg
      901 ccgaggatga taagcggaaa cgggaagagg ccgatgcggt aaatgaggca gacagtctga
      961 tctacaccac cgataaaacc ctgaaggata tcggagataa agccgataaa tctcatgtgg
     1021 agaagattca gaaggccaaa gaggagctgc aaaaggcggt aacagccaag gatatcccgg
     1081 agatcaagac caagaaggaa gccttggaaa aagctttgca tgaactctcg gctactatct
     1141 ataatcagtc cgccggtcaa gccggggctc agggcgctcc cggggcaggg caagagggcc
     1201 cgactggtta cggcccgcag aacgaaggcc caaccgttga cgccgattat aaagttcagg
     1261 atgacaagga caaataaaat accatctgta tccgattagg cctgcgggta cgcagggaag
     1321 atttttaaac tttatgattc tgtaatcaga tccactggat tcggcaagtt tcaattggga
     1381 aagcttttga aatcacttgc cgaattcagg tttatcggat aattcgcgcg tgtgcgcgcg
     1441 taataaagga ataatacgaa tggcaaaacg cgattattat gaggtactgg gggtcggccg
     1501 cgaagctagc gaggaagaga taaaaaaggc cttcagacag ctggcacgga agtaccatcc
     1561 ggatgtcaac aaggataatc ccaaggaagc cgaggctaaa ttcaaagagg tcaatgaggc
     1621 ttacagcgta ctaagcgacc cccaaaaacg ggctgcttac gatcagtacg gtcatgccgc
     1681 caatgaccct aatttcgccg gagggcaagg tttcggcggc gttggcggcg attttagttt
     1741 cgacggtttc ggcgatatct ttgatatgtt cttcggaggc ggcggtggcg gtcgacataa
     1801 gaccgggccg caacggggga acgatctccg ttttgatctc gagatcagct ttgaagaagc
     1861 cgccttcggc aaggagacaa ctgtcgagat tccgcggacc gagatctgcc ccaactgtaa
     1921 cggtaactgt gccaaacccg gtactccgat caaaacctgt ccggcatgta acggtacggg
     1981 acaggttcag gtgactcaaa acactgcttt tggccgtttt gtcaacgtcc ggacttgcga
     2041 gcgttgtcat ggggcgggta agaccatcga aaccccgtgc cccgaatgcg gcggctccgg
     2101 tagggtacgt aagaaccgga agattgacat taagataccc gcaggggtgg agggtggttc
     2161 acggctccgg gtggcgggtg agggagaagc cggtttgcgc ggtggttcgc cgggggatct
     2221 gtatgtttat atttatgtta aaccccatcc caagtttgag cgccagggaa acgacgtgat
     2281 aagcgagatt aaactcagtc taacccaggc ggcgcttgga accacggtaa tggtggatac
     2341 tctcgacggc cgggttgagt tgaaaattcc cgagggtact caacacggga cctctttccg
     2401 tatgcggggc catggtattc ctcatttacg tggccatggc cggggcgatc accacgtccg
     2461 ggtcaaggtg acggttccta ccaaattaag cggtgagcaa cgggaactcc tgcagaaact
     2521 ggcccattcc ttcggtgaga atgtggatga cgacgataag ggctttatgg gtaaggttcg
     2581 tgatgcgttt aaatagacgg ctcttgcata aacaaggtac gagtcgatta ggaggccttc
     2641 atggcctcct ttcatttttt cggtaaaaag cacaaagcct aaaaaatttc gtgtgtttgg
     2701 tgattttcgt agttaaaaag ttcgtatata ttacattcac aacgaaacac acaaaagaca
     2761 cgaaaatggg tatggtttta tttatgccat tgagtggagt ggtgtagtcc ggactgtttt
     2821 ttgatataat gatggaaaac cgatcctgga aggaagcggt ttgtagtttt gcgtgcaagc
     2881 acgcggcaag aatcgaataa aaagtgggcg atagggaatg agcggatctt attggagcga
     2941 gattaaattg gaagtcccca aggaatgggg ggaattggtc gccgagatct tccaggaaga
     3001 gggcgccggc ggtgtgattt atgaagaccc cgaaatttta tcggaacgtc cgtttcaaga
     3061 tgatgaatac ctgggggatg aagctcaggc tttgatcccc aggcgtttcg gtctaaaggt
     3121 ctattttccc gtggatgatc gcctggggga acggattgaa cgggtgaaac agcgaatcgg
     3181 gcagatcctg gagtccgttc cggagtttga actccgccag gttcaggagg aggactgggc
     3241 cgaggcctgg aagacttatt ttaagccgga gcatatcgga tcggtcgtga tcaaaccgag
     3301 ttgggagagt taccagccgg tttcgggaga aatcgtggtg gaactggatc cgggaatggc
     3361 atttggaacc ggaacccatc ctacaacccg tctatgcctc tccctgcttc aagagcaagc
     3421 tccgggcagg aacaatatgc ttgatgttgg gacaggatcc ggcattttgg ctgtgactgc
     3481 cgccaaattg ggggttgggc ggatcgtggc cagcgatatt gatccccttg cggtcaaggt
     3541 ggctacggag aacgtccaac gcaacggagt gtgcgatcag gtgcgggtag tcgaaggaga
     3601 tctgttacgg cttaagctaa ataccccctt tgaacttgta gtcgcgaata tcattaccaa
     3661 tgccatttta aagctgttgc cggatctctc aggtgtgctg gctccgaatg gccgtttttt
     3721 ggcttctggc attatcgacg aacgttttcc cgaggttgaa gccgctctga gaggcctagg
     3781 cttcatgctg gtaaagacgg tcactgcgga aggatggacc gctttggtgg cacagcggag
     3841 ctcttcaact atatcgtagg atgcagactg tttaatccga gaacaacggg ggtttggggt
     3901 ccgatgacgc gttttttcat tccatccagc cagatcgaag gaaatagggt ttttttacgt
     3961 ggagccgacc ggcaccatct gttaacagtg ctccgggaag gtcctggcgc cgagatcatg
     4021 gtcttaaacg gcagagggga agagtttcag gcgaaaatca cggcaatcag tcccgatgca
     4081 gttgaggccg agatctgccg ccgttcggag cgagtcgtgg aaccgagact ccggatcaat
     4141 ctggcccaaa gtcttcccaa agccgataaa ttcgaatgga ttattcagaa gaacaccgaa
     4201 gtgggggtgg cccgttttca gccggttctc tccgaaagga gcaacatccg gttggatcgg
     4261 gatacgacag caaaaaaaat ggaacgttgg cgccggatca ttcaggaagc cgcagaacag
     4321 tccggccggg cggttttgcc ggagttggaa ccgatccgga gttgggaccg gtttttggaa
     4381 gaactgcggc cgggattgat cctcatcccc tgggaaggcg agcaaacccg ctccctccgc
     4441 gaaattttat ctcgtaagca accggcggat ggtgctgcgg cctggccggg agaggtaacc
     4501 gtgattatcg gccccgaagg gggattttcc ctggatgagg tggctgaggc tcaaagcagg
     4561 ggagcgatcc cggtcaccct gggaccccgg attttgagga ccgagaccgc gggactcgtg
     4621 gttgccgcag ccttattgta tcattttgac gatctgggct gaagtcagtt cacttcttgg
     4681 gcgggcgagg tcttttgctg acgaagcttg cggcggaggg gatcgtaaat caacagctgc
     4741 cgggtacccg gcagatcgaa gggttccacc gtatagttca ccaaaggcag ataacagaga
     4801 ctgatattgc agatttgaat gcggatcgcc ggttgaaact tgcggtatat ctcggcggcc
     4861 cgctgataga aaccggagtg atcggcatcc ggcgtttccg tccgattttc ccgataataa
     4921 gcctccaggt atctaagttc ttcctcccag cgggttttgg ccgactccag ccatttccgg
     4981 tcatggtttt ggagtagcca tttcaaatga ttgaacaagg cttcaaatgc gtcacgatag
     5041 gatatgttcc gctttatcgg ccggcgcggc ggctcccgta acggcttccg ggacgtcaat
     5101 tcccagagga gtccggaacc ga
//
LOCUS       DASVMB010000122         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_161741,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000122 DASVMB010000000
VERSION     DASVMB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161741"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VF531_03885"
     CDS             <1..575
                     /locus_tag="VF531_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713140.1"
                     /translation="RDPFWSPDGKRIVFSRGHGPYQAQLGLLNPADGSVKLLYGGDGE
                     LISSPYWGRDGKIYFTKQSPAAKTVTVSGQNYQVMDFGRGSIWAINPEDGKTTAVIED
                     EYDNRIPALSPDGTRLAFVSDRPTLKEGNGKFDRGSLFIKDLKSGRIYLVTTKVGLNG
                     GSLSFSPDGKKLAFFTFRSIRPSVWVVNLP"
     regulatory      728..810
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            917..1399
                     /locus_tag="VF531_03890"
     CDS             917..1399
                     /locus_tag="VF531_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713141.1"
                     /translation="MRRFSLASLFLAVLIFSSLSWAATSHDVTVSISPFARLSVTGTV
                     AFQVDPNDFDQNGFASKEVADAVQLTVKTNSSTGCMVSASGTDFTGGAAKFSIGALKV
                     KIGSGNYVPLSNSPAQVLQIQGVKNNTTYPVTFQLNLDGSEPAGNYSATVTYTVAANS
                     "
     gene            1480..2280
                     /locus_tag="VF531_03895"
     CDS             1480..2280
                     /locus_tag="VF531_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713142.1"
                     /translation="MARKSYGIVLYVILFIMVLLPVRALDLGISPPDVNLKLNSGQVY
                     QGEVYIFGSDEETMAINASLMDWSLSSTGEYQFLPLGTLKRSASPWITFNPWNFNLPP
                     KRGQKITYTIKVPTDASGSYWGTIIFNTNPTTIAGKDHYQVKVAGRIAFIIRIDINGS
                     PLGGGSIERFHLDWNPTARTLNAALRVKNSGVSFIRFKGRLELKDKQGQIVGKFPFKE
                     GYVLPDSSREFTLSSAKLQLAPGFYVGLAVTDLGDRSLKAVQTSLEIP"
     regulatory      2341..2437
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2418..2945
                     /locus_tag="VF531_03900"
     CDS             2418..2945
                     /locus_tag="VF531_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713143.1"
                     /translation="MTAGPPKWVVFLFVDLLSVPGQGSVGLMLGYERLLQHRLWRAIV
                     GVILFGLIIGAGLSEASESTAITVQIAPICRYRIENSRIRRSDDSGKLIQETEIAVLS
                     NSERPWVLVLSSGRNAAGLEWSSDGLNWDEVGENTVILSGTRSFWKRYFVYFRQTEAG
                     VPHTGFMNYQILFEG"
     gene            complement(3055..>3341)
                     /locus_tag="VF531_03905"
     CDS             complement(3055..>3341)
                     /locus_tag="VF531_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713144.1"
                     /translation="IQPLTIFVNPEMGYIGDTINVTVECQLPLTSLELQFPDATMTRL
                     KISNPDTRKSYSWKITGQTPKGQVKIFCRAQGLNGATYQTEANLIVLSKE"
BASE COUNT          819 a          701 c          862 g          959 t
ORIGIN      
        1 atcgggatcc tttttggtcc cctgacggca aacgaatcgt ttttagtcgg ggtcatggac
       61 cttatcaagc tcagcttggt ctgttgaacc ccgccgatgg atccgtgaaa ttgctttatg
      121 ggggggacgg agagctcata agttctcctt attggggaag ggatggaaaa atctacttca
      181 ccaaacaatc accggctgcc aagactgtga ccgtatccgg ccagaattat caggtcatgg
      241 attttgggcg ggggagcatt tgggcaatca atcccgaaga tggcaaaact acggcggtga
      301 tcgaggatga gtacgacaac cggatcccgg cgctttcccc ggatggaacc cgtctggctt
      361 ttgtctccga tcggccaact ttgaaagagg gaaacggtaa attcgatcgg ggcagcttat
      421 ttattaagga tctcaagtcc ggacggatct acctggtcac taccaaggtc gggttaaatg
      481 gcgggtccct gagtttcagc cccgatggga aaaagttggc ctttttcacc ttccggagca
      541 tccggccttc ggtgtgggtg gttaatcttc cgtaatgcaa tcatttcatt tgacacaatg
      601 aaagccattt tgtatttctt ctcttttcga tgtggtatac aaatttgtaa tcgtttcctc
      661 cattgacatg gaaacaattg tttggtaata ttcactatga tgattttggt tcatttgaga
      721 aaacatataa ttataatgcg gtaatgatac cagatttgta tgtggtcact taaatctggt
      781 tgaaccagta gtgaaaccgg cgaaaattta ttcggcggtt ttttgttttg cctcggtgtg
      841 ggttgaatcc tgcgttcgaa ggctgtgaat ttattcttta cttcaatgat ttccaacatg
      901 attaggagga tgttttatga gacgtttcag tttggcatca ttatttttgg cggttttgat
      961 tttttccagc ctctcctggg ctgcgacatc ccatgatgta actgtgagca tcagcccgtt
     1021 tgccaggctt tcggtgacag gtacggttgc attccaggtg gatccgaacg atttcgatca
     1081 aaatggtttc gcgagcaaag aggttgccga tgcggttcaa ctcacggtca aaacgaatag
     1141 ttccacaggt tgtatggttt ccgcttcggg tacggatttt accggtggtg cggccaaatt
     1201 ttcgattggc gctttaaaag ttaagatcgg tagcggcaac tatgttccgt taagtaatag
     1261 cccggctcag gtacttcaaa ttcaaggggt taagaacaac accacctatc cggttacatt
     1321 ccagttgaat ctggacggca gcgaaccggc gggtaactat tccgcaacag ttacttatac
     1381 tgtggccgct aactcctgag tatattttta accattcata atgatgattc tcgagctcca
     1441 tcgaataaag aatcagcctg ataaatgagg aaaaaagcct tggccaggaa atcatacgga
     1501 atcgttctat atgtcatact gttcattatg gtgctactac ctgtccgggc gctggatctg
     1561 ggaatcagcc ccccggatgt gaacttgaaa ttgaatagcg gccaggttta tcagggggag
     1621 gtttatatct tcggttccga tgaggagacg atggcgatca atgcctcatt aatggattgg
     1681 tcgctttcca gtaccgggga gtatcagttt cttccccttg gaaccttgaa acgctcggca
     1741 tccccctgga tcacttttaa tccttggaat ttcaacctac cgccgaagcg gggccaaaaa
     1801 attacgtata ccattaaagt gccgacagat gccagtggca gttattgggg gacaattatc
     1861 tttaacacca acccgacgac gattgcgggg aaggatcatt atcaggtgaa agtggctggc
     1921 cggatcgcct tcatcattcg gatcgacatc aatggttcgc cgcttggcgg ggggagtatc
     1981 gaacggtttc atcttgattg gaacccgacc gccagaacgc tcaatgcggc attacgtgta
     2041 aaaaattcgg gagtttcttt tatccgtttt aaaggacgat tggaactcaa ggacaaacag
     2101 ggtcaaattg ttgggaagtt tccatttaaa gagggttatg tattacccga ttccagccgt
     2161 gagttcacac taagctccgc aaagctccaa ttagcccctg gtttttacgt gggtttggct
     2221 gtgacggatt tgggtgatcg aagtttgaaa gcagtccaaa ccagtctgga aataccttga
     2281 acggcgttct tgtcaaaaaa accggaaggt gttatactat agttaataaa taacaattct
     2341 atccgataaa ggcaaatccg gcgaaagccg gagacgcaaa gctgcgggtc taaaaccagt
     2401 gaaggaattc ctggtaattg acagccgggc cgccgaaatg ggttgttttt ttatttgtcg
     2461 atttgctctc ggtgcccgga caaggaagtg tcgggctcat gctggggtac gaacgtctgt
     2521 tgcagcatag gctatggaga gcgattgttg gggttatcct tttcggcctg attattggtg
     2581 caggattgtc agaggccagc gagtcaactg cgatcacggt tcaaatcgct ccgatctgtc
     2641 gttaccggat tgaaaattcc cggatacgaa ggagtgacga ttccgggaag ctgatccaag
     2701 agactgaaat agctgtttta agcaattccg aacgcccctg ggtgttggtg ctttcttccg
     2761 gccggaatgc cgcaggtctt gaatggtcca gtgatggttt aaactgggat gaggtcgggg
     2821 aaaataccgt gattctatct ggaacaaggt ccttttggaa acggtatttt gtttatttcc
     2881 ggcaaacgga agccggcgtt ccacatacgg ggttcatgaa ctaccagatt ttatttgagg
     2941 gttagaagat agtatcgaag gcatttcaat attgagattc agagaattaa acctcctcct
     3001 tacataattg ttggccttcg ggctaaatct cccgcttggg ggattttttt tgtttcattc
     3061 tttgctgagg acgatgagat tggcttccgt ctggtaagtc gcgccgttta gcccctgcgc
     3121 cctgcagaag atcttgacct ggcctttggg tgtttgtcct gttatcttcc atgagtatga
     3181 tttacgggta tcgggattgg atattttcaa tcgggtcatg gtggcatccg ggaactgtag
     3241 ttccagggaa gtcaaaggta gttgacactc aacagtaaca tttatcgtat ccccgatata
     3301 acccatttcc ggattgacga aaattgttaa gggttggatt t
//
LOCUS       DASVMB010000123         7922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_678613,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000123 DASVMB010000000
VERSION     DASVMB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678613"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1129
                     /locus_tag="VF531_03910"
     CDS             308..1129
                     /locus_tag="VF531_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713145.1"
                     /translation="MLDLQYVRANPEEVRELCRRRKATVDLERFFDLDRKLREVTKKL
                     DDYRHRLKELAGPENRERVLELKRETVLLEKEQQSDRAERDELWALLPNRMAPDTPEG
                     EDDSGNVELARWGEMPRFGFDPKTHEELGAKLGILDLERGAKVAASGFYYWRGDGAKL
                     AWAVFRLAMDFLVERGFEPFFTPLLAKKRTFFGETHSNTNLLDYQTRRLNIRCKNGTD
                     VFHPHTISATMITDRTVLALLENNQQADGSVKIPEALRGYMNHQEQISLREDGAK"
     gene            1155..1655
                     /locus_tag="VF531_03915"
     CDS             1155..1655
                     /locus_tag="VF531_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713146.1"
                     /translation="MGRDVQEIHEVTQEIELEESCRVIREAFATVAAEFGLTVQNNPT
                     NPAFIGFDNLQAMQAKGVRLFGMYEGGEQIGFAAIEKNDDNLYYLEKLAVHPKHRHRG
                     YGKGLIDFTVEYVRNNGGKKIGIGLIDENRVLKDWYYRYGFMETGTKHFQHLPFTVCL
                     MELILE"
     gene            1710..2063
                     /locus_tag="VF531_03920"
     CDS             1710..2063
                     /locus_tag="VF531_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713147.1"
                     /translation="MSPSKSEIIDLNLDLSEIKNIEDYRALWQRMGKIEIKMVEQHEE
                     CKHHLGETFIYETPYQKPANVCNALLHVLDLYIWRVALGFPSWNEDNWAVHKIHCPDP
                     KGTVWEMRKITDEKS"
     gene            2281..3810
                     /locus_tag="VF531_03925"
     CDS             2281..3810
                     /locus_tag="VF531_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713148.1"
                     /translation="MPYYIPQMKKAIDLTITRVHQAIYTPVAELRIVAWVTPAPMGRE
                     IPFASRKNGEKKLLKVGERWGNPGDYAWFEFSGEVPRAAAGKKIVLLVDLGGEGLVFD
                     DHGHPVQGLTNRHSEFDRSLGEPGKRVVEITHQARGGEKILLWVQATTNDLFGKYRDC
                     GKVQEASVAVCDDRMKQLYYNLEFLNDFVQHLPDGDIRAESVLFRLYDATSLLNVLTP
                     QTMAHAEKILDQELSQKGGTPTLTVSALGNSHIDLAWLWPWEETVQKGARTFSTALMM
                     MERYPDYRFGASQPQLYQWIKTAYPELYQRIGRRVEEGRWEPLGALWVESDTNLPGGE
                     SLVRQMLYGKRFFKQEFNREMRIVWLPDSFGFSGALPQIAKKAGVDYFCTTKLSWNLY
                     NHFPHHTFIWQGIDGSRLLAHMPPEGTYNSSAAPRAVIAAEQNYIDKGVSNQILMLFG
                     IGDGGGGPGEEHLEWLKRERNFDCMAPVVEQSALEFFRMLERDQFRYRLWDGELYLER
                     H"
     gene            3823..4074
                     /locus_tag="VF531_03930"
                     /pseudo
     CDS             3823..4074
                     /locus_tag="VF531_03930"
                     /inference="COORDINATES: protein motif:HMM:NF020822.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4267..5301
                     /locus_tag="VF531_03935"
     CDS             4267..5301
                     /locus_tag="VF531_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7713149.1"
                     /translation="MAESRILENDRIRVEFAADGSITAIYDKENDRSVLAPGSCANRL
                     AVYEDDGDAWDIPIGYADRVPEFFQLSQSVHCTDGPKGWIKQTYTYGKSFLEQEISLT
                     HGSRRLDFVTRVDWRERRKMLRTAFTVNVRNTETTCEIQFGSIRRPNTRNTTWEMAKY
                     EVCAHKWIDLSDEGYGVALLNDCKYGHKVYENILDINLLRSPSYPDPEADAGLHEFAY
                     SLFPHQGNHLAGHVARAGYELNIPLNLLALAATSGKLPNCYRLIEVTAENVIIETVKW
                     AEDEPAMIIRLYESGGKATKTGVRIYAQICSIELVDLMENRCDQSKLVNGTELSFNAF
                     EIHTLKVRFE"
     gene            5451..6017
                     /locus_tag="VF531_03940"
     CDS             5451..6017
                     /locus_tag="VF531_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713150.1"
                     /translation="MDNRSELLFTALQMFASRGYDAVGVQEIVAGAGVTKPTLYHYFG
                     SKRGLLGAILTEYGLQLYEMIDENSVYQGDLSLALEKLAMAYFRFAKVHETFYRMQLA
                     MYFAGPESEPNRMVKQMNEEQIRLVEALFIQAAKNHRRMRDRQLALAATFVGTINTYI
                     GLALNGYIELNEDNAQQAVYQFMHGIYA"
     gene            6152..6982
                     /locus_tag="VF531_03945"
     CDS             6152..6982
                     /locus_tag="VF531_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7713151.1"
                     /translation="MRNLRGKVAVVTGAGSGIGRALAVGLSREGTDLALCDIDGTGLE
                     ETGSIILKENFRGRLEVYQVNVARREAVEEFAHEVIRDFGGVDIVINNAGVSSSGGVE
                     ELTYETLHWTIDINLWGVIHVTKAFLPCLRRSPEASIVNLSSVYGLLGVPGQAAYCAS
                     KFGVRGFTEALRQELYQSNITVTLVFPGGVKTRIARNSRTDYHLDPETYEAGLRRIEA
                     TFKTTAAEAAQVIIQGIQKKTPRILIGKDAGKIDLLARTRPGSYDRVIAKYLRRVESH
                     "
     gene            complement(7506..7805)
                     /locus_tag="VF531_03950"
     CDS             complement(7506..7805)
                     /locus_tag="VF531_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX7713152.1"
                     /translation="MSGVAVMDKPAFTRDQMTNASDAARNFKEIRKKAKFAPQVILEN
                     GDLDSVILDYDQYERLILHLQELEEEVLSERLEQLEQNPSLAIPWRNNRRSKTNE"
BASE COUNT         2022 a         1804 c         2178 g         1918 t
ORIGIN      
        1 agaattcaat aaaaagcagg gaaacccttg tcgatactca atttctgttt attactagtt
       61 gatcgatgtg atcaaaatga tcgattttac tgaattgatc taatttatcc aagttcctcc
      121 aacttaaggg taatgttaag cgcgattgca gtggaaggat caggccggcc aattgaaagc
      181 ctacaatagg cggaactgat ccctcgcccg tgataacggg agagggccca catctttgaa
      241 taggctgagg ggtgagggcc ggtggtacgg gaaaggatct agactgaatt aaaggagggt
      301 ggtcttaatg ctggatctgc aatatgtccg ggctaatccc gaagaggttc gtgaactttg
      361 tcgcagacgg aaagccacgg tcgacctgga gcggttcttc gatctggatc ggaaactccg
      421 ggaggtcacc aaaaaactcg atgattatcg ccatcgcctg aaggaactag cagggccgga
      481 gaaccgggaa cgggttttgg aactgaaacg cgaaacggtt ctgcttgaga aagaacagca
      541 atccgataga gctgaacggg acgaactatg ggcgctgtta ccgaatcgta tggctccgga
      601 cactccggag ggcgaggatg attcggggaa tgtggagctt gcccgttggg gtgagatgcc
      661 ccgattcggg tttgatccca aaacccatga agagctcggc gcgaaactgg gaattttaga
      721 cctggagcgc ggggccaaag tggctgcgtc cggattttat tattggcgtg gggatggagc
      781 caaacttgcg tgggcggttt tccggttggc catggacttt ttggtggaac ggggatttga
      841 gccattcttt actccgcttt tagccaagaa gcgaaccttt ttcggcgaaa ctcattccaa
      901 taccaatctt cttgattatc agacacgccg tttaaatatc cggtgcaaga atggaacgga
      961 cgtatttcac cctcacacca tctccgcaac gatgattacc gaccggacgg ttctagcgct
     1021 cctggagaat aatcagcaag cggacggatc ggtcaagata ccggaagccc tgcgaggtta
     1081 tatgaatcat caagaacaaa tcagtttgag agaagatgga gcaaaatgag acacaaaagg
     1141 gggagaagag aaacatgggg cgagatgtgc aggagattca cgaggtgacg caggagattg
     1201 aactggagga aagttgccgg gtgattcggg aagcatttgc tacagtagcc gctgagtttg
     1261 gcctgactgt tcaaaataac cctaccaatc cggcatttat cggcttcgat aaccttcaag
     1321 caatgcaggc aaaaggggta aggctattcg ggatgtatga gggcggggag caaattggat
     1381 ttgcagcgat tgaaaaaaac gatgataatc tctattatct ggaaaagctg gctgtgcatc
     1441 caaaacaccg gcaccgcggc tatggaaagg gactaatcga ctttactgtt gagtatgtaa
     1501 ggaataacgg tgggaaaaag ataggaatcg gattaatcga tgaaaaccgg gttttgaaag
     1561 attggtacta ccgttacggc tttatggaga ctggaaccaa gcattttcaa catctaccgt
     1621 tcacagtttg cttgatggaa ctcatccttg aatgagttcg gaatcttggg aggattagaa
     1681 caaaatggga aataaggagg caactaaaca tgagtccatc aaaatcggag atcattgatc
     1741 tcaacctgga tctatcggag ataaaaaaca tcgaggatta ccgcgcctta tggcagcgga
     1801 tggggaagat tgagatcaaa atggtcgagc agcacgagga gtgtaaacat catctcggag
     1861 aaaccttcat ttatgaaacg ccttatcaga aaccggccaa tgtttgcaac gcgctccttc
     1921 acgtcctgga tttatacatc tggagagtgg ctctgggttt cccctcctgg aacgaggata
     1981 actgggcggt gcataagatc cattgtccgg atccgaaagg gacggtctgg gagatgcgaa
     2041 agatcacgga tgaaaagtcc taggtgaact gaatgtcgat ctaccctttc ccattaaagt
     2101 ggagttgtat cacaactctt aagaaaacgg tgtcaccgtt ttcagggaat cggtcagcat
     2161 cttgggtcaa tagataggtg ggttttggac agatgtgaat ctcgtccatc ataacctgga
     2221 ggtaacggga atatgatttc tctgaattta tccggtacag gttaagatgg aggtcacggc
     2281 atgccttatt atatccccca gatgaaaaaa gcgattgact taactatcac ccgggtgcac
     2341 caagcgatct atacacctgt cgcggagttg cggatcgtgg cctgggtaac tccggcaccg
     2401 atggggcggg aaataccctt cgcctcccga aaaaacggtg aaaaaaagct gctcaaggtg
     2461 ggggaacggt ggggcaatcc cggcgattat gcctggtttg agttttctgg ggaggtgcct
     2521 cgggccgcgg cgggcaagaa aatcgtatta ctcgtagatc tgggcgggga ggggctcgta
     2581 ttcgatgatc acggccatcc ggttcaggga ctgaccaacc ggcattcgga gtttgaccgc
     2641 tctttgggtg aacccggcaa gcgtgttgtg gaaataaccc atcaagcccg gggcggtgag
     2701 aagatccttc tttgggtcca ggcgacgacg aacgatctgt tcgggaagta tcgggattgc
     2761 ggcaaggttc aagaagccag cgtcgccgtt tgcgacgacc ggatgaagca actctactac
     2821 aatctggagt tcctcaatga ttttgttcag catctcccgg atggggatat ccgggcggaa
     2881 tccgtcttgt ttcgcctgta tgatgccacg agtctcctaa acgtgctgac accgcaaacg
     2941 atggcacacg ccgaaaaaat tttggatcag gaactgagtc aaaaaggtgg cacccccacg
     3001 ctaacagtga gcgccctggg aaattctcat atcgatctgg cctggctctg gccttgggaa
     3061 gagacggtgc aaaaaggcgc gcggactttc tccacggcgc taatgatgat ggaacggtac
     3121 cccgattacc gttttggggc cagtcagccg cagctttacc agtggattaa gaccgcctat
     3181 cccgaattgt accaacggat cggccgccgg gtggaggaag gacgctggga accgctgggc
     3241 gcgttatggg ttgagtctga taccaattta ccgggcggcg aatctctcgt ccggcaaatg
     3301 ctttacggga aacgtttttt caagcaggag ttcaaccggg agatgcggat cgtctggctg
     3361 ccggacagtt tcggttttag cggcgctcta cctcagatcg cgaaaaaagc gggagtcgac
     3421 tatttttgca ccaccaagct ttcctggaat ctttataatc actttccgca ccatactttc
     3481 atctggcagg ggatcgacgg tagccggctt cttgcccata tgccaccgga gggaacgtat
     3541 aacagctccg ccgcaccccg ggcagtgatc gctgcggagc aaaactacat cgataaagga
     3601 gtgtccaacc agattctgat gctcttcggt atcggcgacg gcggcggcgg gccgggagag
     3661 gagcacctgg aatggctcaa gcgggaacgg aacttcgact gtatggcccc ggtggttgag
     3721 cagtcggcac tggagttctt ccggatgctg gagcgggatc aattccggta ccggttatgg
     3781 gatggcgaac tttatctgga gcgtcactag gggacatata ccacccaagc ccggaccaaa
     3841 tggtataatc gcaagctgga gctagccttg cgcgatctgg agttttgggc ttcatttgcc
     3901 aggatgttta caggagaacc ttatccggac gcggccatcg agcctatttg gaaagaagta
     3961 ttattttatc aatttcacga cattttggcg ggatcggcaa tccaccgggt ctatcaggaa
     4021 gcgctggtcc gatatgagtc gctgtggcgc gaggttgtat ctttactgaa ccgtgcctgg
     4081 gagagtctgg ccggattcat tgataccact ggtttcgtga aaccgctatt gatattgaac
     4141 tccacctcct gggaacggca ggagtggata aagttccggg aacaatggta ctttgtccgc
     4201 gtaccgtcta tgggctatac gttggtggac actgcctatc ctgcgatcct gcccgccgcc
     4261 ggagtcatgg ctgagtcccg gatacttgag aatgatagaa tccgggtgga gttcgccgca
     4321 gatggttcaa tcacggccat ctacgataaa gaaaacgatc gttccgtatt ggcgcccggc
     4381 agctgtgcca accggctggc ggtttacgag gatgacggag acgcctggga tattccgatt
     4441 gggtacgccg atcgtgttcc ggaatttttt caattgagtc aatccgttca ttgcacggat
     4501 gggcccaagg gttggattaa gcagacgtat acttacggga agtccttttt ggagcaggag
     4561 atcagtctaa cccatggcag ccgcagactt gactttgtaa cccgggtgga ctggcgggaa
     4621 aggcgaaaga tgctgcggac cgcttttacg gtaaatgttc ggaataccga gaccacttgc
     4681 gagatacagt tcggcagcat ccggcggccc aatactcgca ataccacctg ggagatggca
     4741 aagtatgagg tctgcgctca taaatggatt gatctctcgg atgaggggta cggcgtggcc
     4801 ttactcaacg attgcaaata cggacataaa gtctacgaaa acatcctcga tatcaatctg
     4861 ctccgcagcc cgagttatcc cgatccggag gctgatgccg gactccacga atttgcctac
     4921 tccctgtttc cccatcaagg caaccatctt gccggacatg tggcgcgggc aggatacgag
     4981 ttgaatattc ccctaaatct gctggcgctc gccgccacat ccggaaaatt gccaaactgt
     5041 tatcgtctta ttgaggttac cgccgaaaat gtgatcattg aaacggtcaa atgggctgaa
     5101 gacgaaccgg ccatgatcat ccggttgtac gagtccgggg ggaaggctac caagaccgga
     5161 gtacggattt atgctcagat atgctctata gagctggtgg acctgatgga gaaccgctgt
     5221 gaccagtcaa aactggtaaa tggaacggaa ttaagcttta acgcctttga gatccataca
     5281 ctaaaagtca gatttgaata aatctgcctt cgttatcccg atgccgatgt gttcaataac
     5341 tcatcaaaag tatgattttc gcaatatgag tcaagacttc atcatcaaga ggcctttttt
     5401 ttcatatcat tataccaacc ggtataggag accggatcgg gggcagaata atggataacc
     5461 gttccgaact tctcttcact gcgctccaaa tgtttgcctc ccgcggttat gatgcggttg
     5521 gggttcagga gatcgtggcc ggggccgggg ttaccaaacc cacactctat cattattttg
     5581 ggagtaaaag aggcttactg ggagccattc tcaccgaata tggattgcag ttgtacgaga
     5641 tgattgatga aaattcggtt taccaagggg atctctcatt ggcccttgaa aagctggcca
     5701 tggcttattt tcggtttgcc aaggttcatg agacgtttta ccggatgcaa ctggctatgt
     5761 acttcgcggg gccggagagt gaaccgaacc ggatggtgaa gcagatgaac gaggagcaaa
     5821 ttcggttggt ggaggcatta ttcatccagg ccgccaaaaa tcaccgcagg atgagagacc
     5881 gccagcttgc tttggccgcg acctttgtcg ggacgattaa cacctatatc gggttagctt
     5941 tgaacggata tatcgaactg aatgaagata atgcacaaca agcggtttat cagttcatgc
     6001 atggaattta tgcataaaca tctcatttct ataccgggcg gtataaacag gggtgagtcg
     6061 cttaaagttc aatcattttt ggctgcctga taatctaaag ttcatgtctc caatcgattt
     6121 ttcgggaatc accatttcag gaggggaaag aatgagaaat ttacggggta aggtcgcggt
     6181 cgttaccgga gccggctcgg gaatcgggag agcgctggcg gttgggttat cccgcgaggg
     6241 cacggatctg gcattgtgcg atatcgatgg gacaggtcta gaagagaccg gctcgattat
     6301 cctgaaggaa aacttccggg gacgtttgga agtttaccaa gtgaatgtgg cccggcgcga
     6361 ggcagtggag gaattcgccc atgaagtgat ccgggacttc ggcggcgtgg atatcgtgat
     6421 caacaacgcc ggagtctcat catcaggggg agtcgaagaa ttgacttatg agacccttca
     6481 ctggaccatc gatatcaatc tctggggagt tatccacgta accaaggcct ttttgccgtg
     6541 tttacgccgc agtcccgagg caagcatcgt caatctatca agcgtatacg ggttgctcgg
     6601 tgtgcccggc caggcggcat attgcgccag taaatttggg gtgcggggat tcaccgaagc
     6661 cctccgccag gaactctacc aaagcaacat caccgtgaca ttggtatttc ccggcggggt
     6721 caagacccgt atcgcccgaa attccaggac cgactatcat ctggatcctg agacgtatga
     6781 agcgggattg cggcgaattg aagcaacgtt caagacaact gccgccgagg cggcgcaggt
     6841 gatcattcaa ggcattcaaa agaagacgcc acgaatttta attggaaagg acgccgggaa
     6901 aatcgattta ttggcgcgaa cccggcctgg ctcctacgac cgagtgatcg ccaagtactt
     6961 gaggcgggtg gaaagtcact agactgtcgt ctcaagatgg ttagttgccg cggcacactc
     7021 acaaagagta ttgtctgatg gctgatcagc cgggaccctc gaagccgacc attattcttt
     7081 tccctcaaaa ggaaaacggg tgattgccaa ctacttacgg aaagggattt aggaatggtc
     7141 cttttttggc ttggaaatgg atcttcggtg agcttgtccg ttttgggatg gagctgtaag
     7201 ctttttcata cttgcaagcc tttgatcggc taaccggaaa acgtaatcgg cggcacgttt
     7261 ttcaatcgcc aggatataca ccagacgaag aatgtctacc ttttgattga taatccggta
     7321 aacaatgcga attccggcgt cccgctagtt ttacattttg ggaatttccc aatacctttc
     7381 cgatttcatc ggcccgatat tcgagttctt caaggctttt atccacaatt tttactaaag
     7441 aaccatcaag ttttttatac tcccgatagg ctttgggatg aaaccgaatc tcgaacttag
     7501 gaagatcatt cattggtttt cgaacgacga ttattccgcc acggtatagc taatgaagga
     7561 ttctgttcaa gttgttcaag cctttccgat aaaacctctt cctccaactc ttgtaagtga
     7621 aggatcaagc gttcatattg gtcatagtcc aatataaccg aatccagatc gccgttttcc
     7681 agaatgacct gcggggcgaa cttggctttt ttacggatct ccttgaagtt ccgggcggca
     7741 tcggaggcat tggtcatttg atcccgggta aatgccggtt tatccattac tgcgacacca
     7801 ctcatttttt gtccccctca ttttatgcaa agtaaatcaa attatgcatt taatttcgca
     7861 tacagttcca tgtggtattt gccttaagta tataacagtt tcatcgtttc gacaagaagt
     7921 ac
//
LOCUS       DASVMB010000124         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_603845,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000124 DASVMB010000000
VERSION     DASVMB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603845"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VF531_03955"
     CDS             <1..1100
                     /locus_tag="VF531_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX7713153.1"
                     /translation="RLDHVRWPDQLADSGWECGTEIGYLQSLVSYWRDHFDWRAQESE
                     LNRFTQFHCNVDGIDVHFVHERGKGPYPLPIILTHGWPDSYLRYQKIIPLLIDPVRHG
                     GDPEDSFDVIVPSLPGFGFSSRPKHSGMNNFCVSELWAKLMTEALGYGRFAAAGGDIG
                     SGVTRYLALNHPELLVGIHLTDIGIIKNLMTSDEVELSEEELQYKRSAQEWISHEGAY
                     MSIQSTKPQTLAFGLSDSPVGLAGWIIEKFRAWSDCNGDLQQRFGEDELLTNIMIYWV
                     TNTIGSSTRMYYENTHSLPPMGHIDVPTGIVLFPADIMLPPKEWAVRNLNITRWTSMP
                     RGGHFTAMEEPELLADDIRAFYRPFRTKNKK"
     gene            1499..2002
                     /locus_tag="VF531_03960"
     CDS             1499..2002
                     /locus_tag="VF531_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7713154.1"
                     /translation="MYCSKCGNELPKNDNFCSNCNERVWAKTFRSKADENKQKVIAWI
                     FLIGIITIGIFLVKGIFSPRPTNNSAKTIEIHASVRFTGAQFVITNNDNFDWTNVKFE
                     VNSGFLRGGYVLETPIIAAQNTYTVGALQFAKDDGTRLNPFAVKPQNIFISCETPQGK
                     AFYSGGW"
     gene            complement(2172..2247)
                     /locus_tag="VF531_03965"
     tRNA            complement(2172..2247)
                     /locus_tag="VF531_03965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2212..2214),aa:Ala,seq:ggc)
     regulatory      2473..2675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2851..4686
                     /locus_tag="VF531_03970"
     CDS             2851..4686
                     /locus_tag="VF531_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7713155.1"
                     /translation="MKRLIIKSLRYPKLLSVVLSLGMLLAVVSPLWAEEGQTSDGTNT
                     YEEEITVTATRTGQNESTSPGTTEVIGKDEAESANSQTVADAVTDQGITCPSNGGASA
                     VASVQLDGASASQTMVMLNGIPLNEGSTGQVDLNFFPMAGVEKIEIAHGPLSALYGAN
                     ALGGVVNIITDLTGTPSQTVTLGGGSFQNRIVGLELRENQWGLAAGGNWSTGYRTHSD
                     TVSGYFSGQYDLWEQGKEYVRLHGLFLSREADIPGSILYPSQGHQTDEDLMLDLSGRR
                     NYLGGDWEYKLYGQSVQNGFVDQSGMSGDSNNQASRYGFDLAGSYELADHQLLGGIQY
                     RFDDYESNMTGSHQQNGVGLFAQDIWTLNGGWTLTTGLRQDYQTDFQSPPTPRINLTK
                     TVNANFDLKLGYGESFRAPTINDRYYNPGGNPNLRPELGKRYDLSGEWHDGTERLTVG
                     YFYANLLDGINWAPNGGIWTVGNIDKMATNGFNLSWESTWNDWLTGNVRYTWLDKEGW
                     DPVAGSFSRDLNTFGRQQLNLGLRLRLGSQVSTVLNWRLVSGRSQSGTAMPDYNTGDL
                     NIQYQPAAHWSLAFQASNLTNQSYEINQGYPMPGQAFMLTAGYAF"
     gene            4708..>5428
                     /locus_tag="VF531_03975"
     CDS             4708..>5428
                     /locus_tag="VF531_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7713156.1"
                     /translation="MFRNCLIGSICLHILILVGVGLCLPRLSHPSPQSVVYFDVVGSS
                     KSDQASSEGSGNGPAGEAGDNLAVKPGVEANPSRRSPIVSPGSVKPEPDSQTSPDSTV
                     LTAAGEGPGTPGPTETPGTAVQSGSPVEYASGTGNSMAALGGLGAGNHGNGSPGMGET
                     GDPKIYGAGGVEGAAPDISPQKTYQAAPVYPEAARRNNWEGDTVLQALISSDGKPGSI
                     TVVSSSGHPQLDQAAIRAVRRW"
BASE COUNT         1413 a         1252 c         1389 g         1374 t
ORIGIN      
        1 acagactgga tcatgtccgc tggcccgatc aattagcaga ctccggttgg gaatgcggta
       61 ctgaaatagg ttatttacaa tcgctcgttt catattggcg ggaccatttt gattggcgcg
      121 cacaggaatc cgaattgaac cgttttactc agtttcactg taatgtcgat gggatagatg
      181 tgcacttcgt gcatgagcga ggtaaaggac cttatccttt accaattatc ctcacccacg
      241 gatggcctga cagttatcta cggtaccaaa agattatccc tctgcttatt gatccggttc
      301 gtcacggcgg tgacccagag gactctttcg atgtgattgt cccttcacta cccgggtttg
      361 gtttctccag ccgtcctaaa catagtggca tgaacaattt ttgtgtttcg gagctctggg
      421 ctaaactaat gactgaagca ctcggctatg gaagattcgc tgctgcaggt ggggatattg
      481 gctcgggtgt tacaagatat ctggcattaa accatcccga acttctagtc ggaatccacc
      541 tgaccgacat cggtatcatt aaaaatctca tgacttcaga tgaggtagaa ctttcagaag
      601 aagaactaca atacaagagg agtgctcaag aatggatttc ccatgaggga gcctatatgt
      661 cgattcaatc gacaaaaccc cagactctgg cgttcggact ttccgactca ccagtaggtt
      721 tggctggttg gattattgaa aaattccgcg cctggagcga ttgtaatggt gaccttcagc
      781 aaagatttgg cgaggatgaa ctgctcacaa atataatgat ttattgggtt acaaatacga
      841 tagggtcatc aacacgcatg tattatgaga acacccattc tttgccgccg atggggcata
      901 tagatgtccc gacaggtatt gtgctcttcc cggctgatat catgttgcca cccaaggaat
      961 gggcggtgcg caatctgaat ataactcgct ggacttcaat gccccgcggt ggacatttta
     1021 ctgccatgga agaaccagaa cttctagccg atgacattcg cgcattctac agaccattta
     1081 gaaccaaaaa taaaaagtaa tcatgaattt actttttatc ttattctgct atgccttgag
     1141 gagacaaaaa agatgccgtt tgagcctggt agccccttgg cggcatcata tttttgaatc
     1201 agtcattcaa atccatgccg gatatcccat tcggatgaat cggtttatta ttttgataag
     1261 gaagagctga ggatgttcaa ggccaaatgt ttgcaatgtt gctgcggcca aattgaagaa
     1321 gtcaaatact gtccggttac aaggtgcccg ttgcataaat accgggaaca atactagaag
     1381 aagtaatgca taggaggcta ttagaaaatt tcgagtagca tttatcttta tactaatttt
     1441 gtaaatggga ggatattcaa aatatctcta gaattgtctc ttaaaaggag gcataaatat
     1501 gtattgttct aaatgtggaa acgaactccc caaaaatgat aatttctgta gtaactgcaa
     1561 tgagagagtg tgggcaaaaa cttttcgctc taaagcagat gaaaataaac aaaaggttat
     1621 tgcgtggatt ttcttaattg gtattattac aattggtatt tttcttgtta aaggtatatt
     1681 ttcaccaagg ccgacaaata attcagcaaa aacaatagaa atacacgcaa gtgtacgatt
     1741 tacgggagct caatttgtaa taactaacaa tgataatttt gattggacca atgtaaaatt
     1801 cgaggttaat tccggatttc ttcgaggagg ttacgttcta gaaacgccca ttatcgctgc
     1861 ccaaaatact tatacagtcg gagctttaca atttgcaaaa gatgatggaa ctagattaaa
     1921 tccttttgca gtaaaacccc agaatatatt catatcttgc gagactcccc aaggaaaagc
     1981 tttttattct gggggttggt agaaaaaagt ctgaggttat aagtaaagaa tatgaactta
     2041 attcgatggt atgaagaaac tcagtacttt gttaagtttc acccttcacc cttcaaactt
     2101 cacacttcag aaaattaaaa cccgaaatga aaaaacccgc agcatacgct acgggtttaa
     2161 attttcgttt ttggtggacc tgaagggaat cgaacccttg acctcttgaa tgccattcaa
     2221 gcgctctccc aactgagcta caagcccact tgtgcaacca gcgtcaccgg agaccgaagt
     2281 gctccgttcg agtgacatat aataatatac caaagaacgg acccctttgt caatagctag
     2341 ttcgtgctat atttttccgg cgaagcgact ttcggcggca gggcgggtgt caatgagcgg
     2401 agatttccgg taggccctag gatgatttta tctcccgggg aagccttgaa tcgaaacccc
     2461 agcaatgata taatgaatta caaaacttca ggcgccgtcg aattgtcttt aattcttaaa
     2521 gacgctgcgg cttaataggg aaacaggttg aaatcctgta cggtcccgcc actgtgttag
     2581 gggagtttcc tccggtatgc cactggttcg ccgggaaggc gtgaggaaat gaggatccta
     2641 gagtcaggag acctgcctgg agtcgcgctc gttggagcgc gcgcgtgcgt atacgcacaa
     2701 cattttcccc tacgggagat aggaggatgt catatgattg atccgcataa acccaacatc
     2761 attccacatt gattgatgtc gggtttttat tttacctggg tttagctgga aaatagtaat
     2821 agagattggg aaaacgaagg aggagccgtt ttgaaaagat tgatcatcaa atcgttacgt
     2881 tatccaaaac tactttccgt ggtattgagc cttggaatgc tcttggccgt tgtcagcccg
     2941 ctttgggccg aggaaggcca aacctcggat gggacaaata catatgagga agagatcacc
     3001 gtcaccgcca cccgcaccgg gcagaatgag tcgacttcgc ccggaaccac cgaagtgatt
     3061 ggcaaagatg aggcagagtc ggccaactct cagactgtgg ccgatgccgt cactgatcag
     3121 ggtatcacct gcccgtcgaa cggcggcgct tcagccgttg catcggtgca actggacggt
     3181 gcttcagcct cccagaccat ggtgatgttg aacggcattc cgttaaatga aggctccacc
     3241 ggccaggttg acttgaactt cttccccatg gccggggtcg aaaagatcga gatcgcccac
     3301 ggaccgctat cggccctata tggagccaat gccctgggcg gcgtggtcaa catcatcacc
     3361 gacctaaccg gtacgccaag ccaaaccgtt accttggggg gcggttcttt tcaaaaccgg
     3421 attgtcggtc tggaactccg ggagaatcaa tggggactgg cagcaggggg caactggtcg
     3481 actggttacc ggacgcactc cgataccgtg tcgggttatt tctccggtca gtatgatctt
     3541 tgggaacagg ggaaggagta tgtacggctg cacgggctct ttttgtccag ggaggccgat
     3601 attcccggtt caatcctata tccctcccag ggacatcaaa ccgatgagga tctgatgctg
     3661 gatctgagcg ggagacggaa ttatttgggc ggtgattggg agtacaaact ttacggccag
     3721 tcggttcaga atggttttgt tgatcagtcc ggtatgtccg gcgattcaaa taatcaagcg
     3781 tcccgttatg gctttgatct ggccggcagt tatgaactgg ctgatcatca actcttgggg
     3841 gggattcaat accgcttcga tgattatgag agcaatatga ccggcagtca ccagcagaac
     3901 ggtgttggac tttttgccca ggatatatgg actctaaatg gggggtggac tctcaccaca
     3961 gggctacgcc aggattatca gaccgatttc caaagtcccc ctacacctag gatcaacctg
     4021 accaaaactg tgaacgccaa ttttgatctc aaacttggct atggcgagtc cttccgggct
     4081 cccaccatta atgatcggta ctataacccc ggggggaacc ccaaccttcg tccggagctc
     4141 gggaaacgtt acgatctgag cggtgaatgg cacgacggga cagaacggtt aaccgtgggg
     4201 tatttctatg ccaacctcct tgacgggatt aattgggctc caaatggtgg gatttggact
     4261 gttggtaata tcgataagat ggccacgaac gggttcaacc tcagctggga gagtacttgg
     4321 aacgattggc ttaccggaaa tgtccgctac acttggcttg acaaggaggg ctgggatccg
     4381 gtcgccggga gtttcagccg ggatctgaac acttttggac gtcaacaatt gaatcttggg
     4441 ttgaggctgc gtctgggatc tcaggtctcg accgtgctca actggcgttt ggtgagcggt
     4501 cggagccaat ctgggacggc catgccagat tacaacaccg gtgacttgaa tatccagtat
     4561 caacccgcgg cccattggtc cttggcattt caagcttcca atctcaccaa tcagtcatat
     4621 gaaataaacc agggatatcc catgcccggt caggcattta tgctgactgc ggggtatgcg
     4681 ttttaacggt ccgttgaggt gaaacgaatg tttcggaact gtttgatcgg ctcgatctgt
     4741 cttcacatct tgatattggt cggggtagga ctctgcctgc cccggctttc ccatccgtct
     4801 cctcaatcag tggtttattt tgatgttgtg gggtctagta agtcagatca agcatcttcc
     4861 gagggttccg gcaatggccc agccggggaa gcgggtgaca atttggcggt caagcccggt
     4921 gtagaagcga atccatcccg gcgatcgcca attgtttcac ctggctccgt aaaaccggaa
     4981 ccggattccc aaacatcacc ggactcaaca gtattgacag cggccgggga agggccaggt
     5041 actcccggac caaccgaaac tccgggcact gcagttcaat ccggaagtcc ggttgaatat
     5101 gcttcgggaa ccgggaattc tatggcggca cttggcggtt tgggggccgg caatcacggg
     5161 aatggcagcc ccggcatggg ggaaactgga gatcccaaga tctatggagc gggcggcgtg
     5221 gaaggtgcag cgccggatat ctctccgcag aaaacatatc aagcagctcc ggtctatcct
     5281 gaagcagccc ggcgcaacaa ttgggaaggg gataccgtat tacaggcatt gatctcaagt
     5341 gacggcaaac ccggctcgat tacggttgtg agttcctccg gccatcccca gctcgaccag
     5401 gccgccatca gggcggtgcg ccgctgga
//
LOCUS       DASVMB010000125         7841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_15720,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000125 DASVMB010000000
VERSION     DASVMB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15720"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..643)
                     /locus_tag="VF531_03980"
     CDS             complement(191..643)
                     /locus_tag="VF531_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713157.1"
                     /translation="MNLFYHSTLAGIIGAAFVNIATYIFRFLGVKTSTPWEIGASVFL
                     NPGFIHTVSGVVIGLTASVALSTAVALVIAYVLKWTGPNQAWLKGIICADGLGFINLG
                     FFMRFLDIWPQIRNEPGTNLMALTVLSILGIIQALLLKNWQHKGIGAR"
     gene            complement(750..1625)
                     /locus_tag="VF531_03985"
     CDS             complement(750..1625)
                     /locus_tag="VF531_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7713158.1"
                     /translation="MNITVILMYWSVILMSELLSFEFVQRGLVGAALIGSICSLIGVF
                     VVLRGMSYIGSGIAHGSLAGVAFALLMGWNPLLLSIIAALLMVILIETISRKATLQMD
                     TSIGVIFSITLAMAVLFIGMLRQYTPDLMSYLFGNLLRITHTELWAMGGIALLVSLLF
                     ILFFKELQFSTFDPEMAEISGIPSALISIMISVLMALTIVVSLQAVGELLVLALIVLP
                     ASTAYQLTRSLKGMMGISVGLGVLASITGLILAFFWDVPTGSTIVMILGVLFFTALGF
                     GKIRNRTKRLTDQNR"
     gene            complement(1576..2376)
                     /locus_tag="VF531_03990"
     CDS             complement(1576..2376)
                     /locus_tag="VF531_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX7713159.1"
                     /translation="MKHCDQAQEPLLIVSGVTLGYPGIIALTNITFTVNQGEFIGIAG
                     PNGSGKSTLLKGILSLLPVQSGVIRYCGYEDRSTRLIRKHIGYVPQKNKTDVHFPALV
                     REVVLMGLYAQIGWVRRPQKIHYQMVLDSLQSVGMADYSEHPIGELSGGQQQRVMIAR
                     ALVSKPRLLILDEPTAAVDIYAQRSILDTLEKLNREKEVTILMVSHDINEIVHSCDKI
                     ILLNRNLSVFGTANEVLTRENLVQVYGDRIYVYEHHGHPHVLVGDFDE"
     gene            complement(2373..3254)
                     /locus_tag="VF531_03995"
     CDS             complement(2373..3254)
                     /locus_tag="VF531_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7713160.1"
                     /translation="MNRRNYTYAFTILLLLCIIGLTLLSETGSTANPNHKISVVTSLP
                     LVKDIVEKIGGTKVTVESIIQGPSCQHEYEPSAGDMKRLAGCAVFVKLGMGSDVWTDK
                     LASGALNKKARFIDASRGIQTLKVRGLVNPHYWGNPGNVKQMARNILNALNEALPQER
                     PYFTANYQSFIAELEKNAAALKAKAATVANKKFVSYSNAFPYFYQYFGFNNIATVELS
                     CEREVTPKEIAVAAKLIQKQKIGVLVGDIVEPNEPDGLAGETHARKILLWATTDGSND
                     YLKTLRHNVTTLVEALQ"
     gene            complement(3716..4129)
                     /locus_tag="VF531_04000"
     CDS             complement(3716..4129)
                     /locus_tag="VF531_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7713161.1"
                     /translation="MEETLHLLQKKGYKLTKQRRLILEALETSAPLSAEEIFSQIHKK
                     CKVNLSTIYRNLNILLKVGIIRKANNMAQADHFELISHHCTHALLCLKCGEKVLFSSC
                     MFDQMVRDIEFQTQYQVKHHNFEVYGLCPKCCLPV"
     gene            complement(4325..4972)
                     /locus_tag="VF531_04005"
     CDS             complement(4325..4972)
                     /locus_tag="VF531_04005"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX7713162.1"
                     /translation="MILILTVIEFLCGSLMFSYWLGLAAGKDLRTVGDGNPGAFNLWQ
                     AAGFKFGIAGVFLDFIKGYLPLAVFITAGYTSGLALVPIAIAPILGHAFSPFLGFRGG
                     KSIAVTFGVWSAVTNFVVSFAYAVILALLMVVAKFYRKGKPTSTETDAFMDVFGMAVV
                     LIGLILMDFPSNLVLLCLLNFLILTYTNRQKLYAFFKLHPTLPRVNNKPPTQSHP"
     gene            complement(4969..6072)
                     /locus_tag="VF531_04010"
     CDS             complement(4969..6072)
                     /locus_tag="VF531_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7713163.1"
                     /translation="MKTIILFILQFLSGFVLFSRTLIRKSRQPLETDKKVSVIIPARN
                     EEQNLPYLLDSLHRQTYKPYEIIVIDDFSEDKTEEIARRHHVQVIKNTELPPGWTGKT
                     WAVWNGYQNSSGEILVFLDADVRLTPRALESLIQTRERVKGVISVVPYHHTEKFYERF
                     SLITNILGVFAFTSPFEKRNPEKGLYGSCIVAARNDYEKINGHHSIKSELLDDLNLGK
                     KFSRAGIPVENYIGWGLVSFRMYPAGIKSEIQGFGKGALLSTATLRPATVLIILFWLL
                     GLFAVEFMTPLLLLTKPSQVAPFVIGYILYTIQIIYFVKYVGYFGRIMPVFHFLSSLF
                     FLLVTLFSFYQVTFLGYVSWKGRHIKIGGGKIC"
     gene            6529..7221
                     /locus_tag="VF531_04015"
     CDS             6529..7221
                     /locus_tag="VF531_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713164.1"
                     /translation="MIYSEEVEKMSCVAKGPNHGPAPIPEEGKWVQAKEIKDISGLTH
                     GIGWCAPQQGGCKLTLNVKDGVIQEALVETIGCSGMTHSAAMAAEILAGKTILEALNT
                     DLVCDAINTAMRELFLQIAYGRTQTAFSEGGLPIGAGLEDLGKGLRSQVGTMYSTLAK
                     GPRYLEMAEGYVTRIALDEHNEIIGYEFVHLGKMMDMIKKGTDANEALKKATGKYGRF
                     DEAKKYIDPRHE"
     gene            7270..>7841
                     /locus_tag="VF531_04020"
     CDS             7270..>7841
                     /locus_tag="VF531_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HEX7713165.1"
                     /translation="MSLFESYERRIGQINTALGKYGIKSIEEAKAICDKKGIDPYTIA
                     KNTQPICFENVGWAYVVGAAIAIQKGCKKAADAAEAIGEGLQAFCIPGTVADDRKVGL
                     GHGNLGAMLLREETKCFAFLAGHESFAAAEGAIGIARSANKVRKEPLKVILNGLGKDA
                     AQIISRVNGFTYVQTKFDYYTGKLSVVREIA"
BASE COUNT         2213 a         1730 c         1874 g         2024 t
ORIGIN      
        1 actttctgcc ggggttgcgt cggcgctttc agccgccaaa atcggtaaac ccgttactag
       61 aaccaatgcc aggataaaca gggaaacgat acttcgtgtc atcatgaaca cctccaaatg
      121 ttttttggtt caaccgctga ttcgatcata ataccatccg gagtttttgt cggtgatcat
      181 gatcacaaga ttatcttgct ccgattccct tatgttgcca gttctttaat aaaagcgcct
      241 ggataattcc tagaatagat aaaacggtta atgccatcag attggtgccg ggttcgttcc
      301 ggatttgcgg ccagatatcc agaaagcgca tgaagaatcc caggttaatg aaaccgagtc
      361 catctgcgca gataattccc tttagccaag cctggttggg accggtccat ttcagaacat
      421 aagcaatgac taaagccact gctgtactta aggctactga ggctgtcaat ccaataacaa
      481 cccctgatac cgtgtggatg aatccggggt tcaaaaacac actagccccg atctcccagg
      541 gagtactggt tttaaccccc agaaaccgga agatataggt agctatattt acaaatgctg
      601 ccccgatgat acccgctagg gtggaatgat aaaatagatt cattgtcctt acctcaattc
      661 aaggatttag gggtatttta tcctgaatga gagctaccat gcggtgtaaa aacaagatag
      721 gtatttgttt tcaaagtcgt taaacaaaat tacctgtttt gatccgttaa ccgcttggtt
      781 cggtttcgta tttttccaaa gcctaaagcc gtaaagaaaa gaactccaag gatcattaca
      841 atggtggaac cggtgggcac gtcccaaaag aacgccagga ttaatccggt aatcgacgcc
      901 aagactccta atccgaccga gatccccatc attcccttta atgagcgggt tagttgataa
      961 gcggtggagg ccggtaaaac gattagagcc aatactaaga gttctcccac cgcttgcagg
     1021 gagacaacaa tcgttaacgc cattaatacc gaaatcatga tcgagatcag ggcggacgga
     1081 attccggaga tctctgccat ctccgggtca aaggttgaaa attggagttc tttaaaaaac
     1141 agaataaaca aaagggaaac caaaagggca attccgccca tggcccataa ttcagtatgg
     1201 gttatccgaa gcaggtttcc aaataagtag ctcattagat ccggagtata ttgcctcaac
     1261 atcccgatga acaggaccgc cattgccaag gtgatggaaa aaataacacc gatggaggta
     1321 tccatctgaa gcgtggcttt ccggctaatg gtttcaatga ggataaccat gagtaaagcg
     1381 gcaataattg ataacagcaa cgggttccaa cccatcagca aggcaaatgc cactccggcc
     1441 agagaaccgt gagcgattcc ggacccgata taagacatcc cccggagcac gacgaaaaca
     1501 ccaatcaacg aacagatcga cccgatcagg gcggcgccga ctaatcccct ttgaacaaat
     1561 tcaaaactca acaactcact catcaaaatc accgaccaat acatgaggat gaccgtgatg
     1621 ttcataaaca taaatccggt caccgtatac ttggactaaa ttttccctgg tcaatacctc
     1681 atttgcagta ccaaaaacag ataaatttct atttaagagt attatcttat cgcaggaatg
     1741 gacgatctca ttgatgtcat gagaaaccat caaaatcgta acctcttttt cccggttgag
     1801 cttttccaat gtatccaaga tcgagcgttg ggcataaata tcgaccgccg ctgtgggctc
     1861 atccaagatt aatagtcgcg gttttgagac cagggctctg gcaatcatca cccgttgctg
     1921 ttgtccgccg gataattcgc cgatggggtg ttcgctataa tcagccatgc cgacactttg
     1981 taaactatcc agcaccattt ggtaatggat tttttggggt cgccgcaccc agccaatctg
     2041 ggcatacaat cccattaaaa ccacttcccg gacgagcgcc gggaagtgga cgtcggtttt
     2101 atttttttgc ggtacataac cgatgtgctt gcggattaga cgggtcgagc gatcctcgta
     2161 tccgcaataa cggatgacac cggattgcac cggaagcaga ctcaagatcc ctttcaacaa
     2221 agtgctttta ccggagccgt tcggaccggc gattccgata aactcacctt gattcactgt
     2281 gaaggtgata ttggtcaaag caataattcc gggataacct aaggttaccc cggatacgat
     2341 caataaaggt tcttgtgctt gatcacaatg tttcattgca acgcctctac cagtgttgtc
     2401 acgttgtgcc gtaaagtttt cagataatca ttggagccgt cggtggtggc ccatagtaaa
     2461 atttttcggg catgggtttc cccggccaaa ccgtcgggtt cattcggttc gacgatatca
     2521 cccaccaaaa cgccgatctt ttgtttttga atcaatttcg ccgcaacggc aatctctttg
     2581 ggggtgactt cccgttcaca ggagagctcc acagtggcaa tgttgttaaa tccgaagtac
     2641 tggtaaaagt atgggaaggc attggaataa cttacgaatt ttttattggc cactgtagcc
     2701 gctttggctt ttagtgcggc tgcgtttttt tcaagttcgg caatgaaact ttggtaattg
     2761 gctgtaaagt aaggcctctc ttggggtaac gcttcattta gggcattaag aatattccgg
     2821 gccatttgtt tcacattgcc ggggttgccc cagtaatgcg gattcactag gccgcgtact
     2881 ttgagggtct gaatgccccg ggacgcgtcg atgaagcgcg ctttcttatt gagggctccg
     2941 gaagccagtt tatctgtcca aacgtcagaa cccatcccga gttttacaaa tactgcacat
     3001 ccggctagcc ttttcatatc accggcagaa ggctcgtatt catgttggca actgggccct
     3061 tgaataatgc tctctaccgt aacttttgta ccgccgatct tttcaacaat gtccttaacc
     3121 aacggcaggc tggttaccac ggatatttta tggtttggat ttgccgtcga accggtttcg
     3181 gagagcaagg tcaaaccgat tatgcataga agcagcaaga tagtaaaagc ataagtatag
     3241 ttccttcgat tcatccattt ggcccctttc agcaaagtat tatgattgag tacggcttat
     3301 cagatctatt tgccggaggg gctcgaaaac aataaccgta tgaccaaacg gcagttatta
     3361 ttccttttag gtgtggagaa acagtgataa taaggattct aaatagaata agaagaaagg
     3421 gttcggatgc aacaaaaccg tttcttgcga tttgacaaac catgcaatgg tcgtcatcgt
     3481 gctcatgccc atatccgaag ggatggttat gcatagcgag aaggaataga gaacagacga
     3541 tgaaaatgac aaataaacga ataaggaatt tcctttgctt catcagatag cctttcctga
     3601 ggatttcccc ggaatgattc gattttaaaa caccatagcc ccatagatat ctgtaattga
     3661 aaaattttcg caattgggag aggacatgtc cagtgtctcc ggtcataagg atgaattata
     3721 ccggtaaaca acattttggg cataaaccat agacttcaaa gttatgatgt ttcacttgat
     3781 attgagtctg gaattcaatg tctctgacca tttgatcaaa catgcatgag gaaaagagca
     3841 ctttttcacc gcatttgagg cagagaagcg catgagtgca gtgatgactt atcagttcaa
     3901 agtggtcggc ttgtgccata ttattggctt tacgaataat gcctaccttg agaagtatat
     3961 ttaaattgcg ataaatggta ctgaggttta ccttacactt tttatggatt tgggagaaaa
     4021 tttcctcggc cgaaagcgga gcagaggttt ccaatgcttc taatatcaaa cggcgttgtt
     4081 tggtaagttt ataccctttt ttttgtaata ggtgcaaggt ttcttccatc gatgatccct
     4141 tcgttttgtt aggattgaga atgattcatg agaattgatt gcagcctaat atagaaaatt
     4201 ttgcaattgc attatatacc ccttcacttc cagcgtcaat aggcatattt cccaaggaca
     4261 aagagattcg ctctctgctg ccggcagatg agattgtgat atcggatggt gataacttgc
     4321 tgtgctacgg atgagattgc gttgggggtt tgttattcac tcttgggaga gtggggtgca
     4381 gtttaaaaaa agcatataat ttctgtcgat tggtataagt taaaataaga aagtttaaca
     4441 agcataataa aacgagatta cttggaaaat ccatcaagat caagccgatt agtaccacag
     4501 ccattccgaa aacatccatg aacgcgtcgg tctctgtgga agtcggtttt cctttacgat
     4561 aaaactttgc gactaccatc agtaatgcca gaatcaccgc atatgcaaaa gaaacaacaa
     4621 aattggtaac tgcgctccat acaccgaagg ttacggcgat ggatttcccc cctctaaatc
     4681 ccagaaacgg cgagaaagca tgcccaagta ttggagctat tgcaatgggt acaagcgcca
     4741 ggcccgaggt atacccggca gtgataaaaa ccgccagtgg cagataccct ttgatgaaat
     4801 ccaggaatac accggcgata ccgaatttga aacctgccgc ctgccataga ttgaaggcgc
     4861 cgggattgcc atcgccgacc gttctcaagt cttttcctgc ggcaagaccc agccagtatg
     4921 agaacatcag ggagccgcat aaaaactcta ttactgtaag tataaggatc aacaaatctt
     4981 tccgcccccg atcttaatgt gcctgccctt ccaggagacg tagcccagaa aggtgacttg
     5041 ataaaaagaa aacagggtga ccaaaaggaa aaacagggaa gacaaaaaat gaaaaacagg
     5101 cattattctg ccgaaatacc cgacatattt aacgaaataa atgatttgaa ttgtgtagag
     5161 aatatatccg atgacaaagg gagcgacctg ggaaggtttg gtcaatagaa gcagtggagt
     5221 cataaattcg acggcaaata gtcccaggag ccaaaagaga atgatgagaa cagtcgccgg
     5281 tcgtaatgtt gcggtgctaa ggagggcgcc ctttccgaac ccttgaatct cactcttgat
     5341 acccgcaggg tacattcgaa aagacaccag gccccaaccg atatagttct cgaccggaat
     5401 cccggccctg gaaaattttt ttccaaggtt caggtcatcc aggagttccg atttgatgct
     5461 gtgatggccg ttgatttttt cataatcgtt tcttgctgcg acaatgcagg aaccataaag
     5521 ccccttttcg gggtttcgtt tttcaaaagg tgaggtgaag gcgaagaccc ccaagatatt
     5581 ggtgatcagt gaaaaccgct cgtagaattt ttccgtgtgg tgatatggaa ctaccgatat
     5641 aacgcctttt accctttccc tcgtttgaat cagcgactca agagcccgag gcgtcagccg
     5701 gacatcggcg tcaagaaaga ccagaatctc tccggatgaa ttttggtaac cattccatac
     5761 ggcccaggtt ttgccggtcc atccgggggg aagctccgtg tttttaatga cttgcacatg
     5821 atgcctgcgc gcgatctctt cggttttatc ctcagaaaag tcatcaatca caatgatttc
     5881 atagggtttg tatgtttggc gatgaaggga gtcgagaaga taaggcaggt tctgctcttc
     5941 attcctggcg gggataatga cggagacctt tttgtcggtt tccaacggct ggcgggattt
     6001 tcgaatcaat gttctggaaa aaagaacaaa ccccgataga aattgaagaa tgaataaaat
     6061 gattgttttc ataatcgatt cttcctttaa tataataatg acggggttat caagatcaaa
     6121 gatatattcc gttgttgcgc tgtaacgttt cacgaataat gtcctattgg aatctatcat
     6181 tattataaca ccgaacggtt tttacagcgt cgggcttgtg tggctcgcca ttatttcatt
     6241 ttatttaaat cctgcagaat tgtcccgttc atcagaagga aattttaggg gaaataatga
     6301 atttatagtc atgattttat ctttgaacct tcaaagcgac ggttgccgtg acgcgggagc
     6361 ccgccgcggc cgcttcaaag ttgttaactc ctatgttagg cgtgttgctt taatgtaggg
     6421 gtttttattt ggaacgttcc atatcatcct gcggtcttga tcacagccgg caatttcgtt
     6481 aagttctcga agggatagat acaaaatgaa tatgaaaggg gtttaaaaat gatctactca
     6541 gaagaagtgg aaaaaatgtc atgtgtggcc aaaggcccca accacgggcc ggctcccatt
     6601 cccgaagagg ggaaatgggt tcaggcaaag gagatcaagg atatctccgg tctgactcac
     6661 ggtatcggct ggtgcgctcc gcaacaaggg ggctgcaagt tgaccttgaa tgtgaaggac
     6721 ggtgtgattc aagaggcgct ggtggaaacg atcggttgtt ccggcatgac ccattccgcc
     6781 gcaatggctg ccgagattct tgcgggtaaa acgattttgg aggcgctcaa caccgatctg
     6841 gtttgtgatg ccatcaacac cgccatgcgc gagttatttc tgcagatcgc ttatggccgg
     6901 acccaaaccg cattctcaga gggcgggttg cccatcggcg ccggtctgga ggacttgggt
     6961 aaagggttaa gaagccaagt cggtaccatg tacagcactt tggccaaagg cccgcgttac
     7021 ctggagatgg ccgaagggta tgttacccgg atcgctctgg atgagcataa cgagatcatc
     7081 gggtacgagt tcgttcacct cgggaagatg atggatatga tcaagaaggg tacggacgcc
     7141 aatgaagcct tgaagaaagc taccggcaaa tacggccggt tcgacgaggc caaaaaatac
     7201 atcgatcccc ggcatgagta atcttgagcc tgggtcattt tgcataaata aataaggagg
     7261 tctataggga tgtctttatt tgagagttat gagcgcagga ttgggcagat caatactgca
     7321 cttggtaaat acggtatcaa atccattgaa gaggccaagg cgatatgcga taaaaagggt
     7381 attgatccat ataccatcgc caagaatacg caaccgatct gttttgagaa tgtaggttgg
     7441 gcttatgtgg tcggcgcggc gatcgccatc caaaaaggat gcaaaaaagc ggcggatgcg
     7501 gccgaggcca tcggcgaagg cctacaagcc ttttgcattc cgggaaccgt ggccgatgac
     7561 cgcaaggtgg gattaggcca tggtaatctg ggggcaatgc ttttaagaga agagaccaaa
     7621 tgctttgcgt tcttagccgg ccatgaatcg tttgccgcgg cggaaggagc catcggcatc
     7681 gccagatccg ctaataaagt acgcaaggaa ccgctcaagg tgatcctgaa cggtttgggc
     7741 aaggatgccg cccagatcat ctccagagtc aacgggttca cttatgtaca gacgaaattc
     7801 gattattaca ctgggaaact gagtgttgtc cgggagatcg c
//
LOCUS       DASVMB010000126        12584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_384202,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000126 DASVMB010000000
VERSION     DASVMB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384202"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF531_04025"
     CDS             complement(<1..1230)
                     /locus_tag="VF531_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX7713166.1"
                     /translation="MERDVFENLSPLDHRYYASNEALFTELGAFFSENAYIRYELKVE
                     AALARVLARRGLCSEAVAAEIATACEEVTPEEVYREEEITKHNIRALVNCVQRRVSDA
                     AKPFVHFTTTSVDIMDSATALRFKDATTQVIVPRSLALERVLITIARREKATLQVGRT
                     HGQHAVPITFGFAMAEYVSRFGDRITAIQAAGSNLRGKISGAVGAYNASSLFFQDPMG
                     FETEVLAELGLKPADYSSQIVAPEYLTDYIHTIISALGVLANLADDIRNLQRTELGEV
                     GETFEATQVGSSTMPHKRNPWNFEHVKSTWKEFMPRMITLYSDQISEHQRDLTNSASG
                     RFVPEIVVGFAEAVQRLTRVLERLVVDEERMSHNFDLTKEMIIAEPLYILLASLGHPD
                     AHEAVRRLTLESQKTGKS"
     gene            complement(1696..2217)
                     /gene="purE"
                     /locus_tag="VF531_04030"
     CDS             complement(1696..2217)
                     /gene="purE"
                     /locus_tag="VF531_04030"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7713167.1"
                     /translation="MPRVGIVMGSDSDLPILKQTAEILEHFGIDFEMTIISAHRSPQK
                     AVEYGSSAVDRGLEVIIAGAGGAAHLPGVLAALTPLPVIGIPIQTAALGGVDSLYSIV
                     QMPSGIPVATVSINGAKNAGVLAVQIVGVADPQLRRKLIEYKHELAEDVEGKAAMLEQ
                     IGYREYLGHKTGK"
     gene            complement(2337..2942)
                     /gene="pyrE"
                     /locus_tag="VF531_04035"
     CDS             complement(2337..2942)
                     /gene="pyrE"
                     /locus_tag="VF531_04035"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7713168.1"
                     /translation="MRLLYCKGETSVLDQKEILGIFRETKVLRDGHFRLTSGRHSRQY
                     MQCAQVLQYPKFTERLCEDLARRFKGQELHAVVAPAIGGIIIAYEVAKVLGVRALFTE
                     REDGKMTFRRGFELDDDENVLIVEDVITTGGSVLEVMEAVKERGANVCGVGILVDRSG
                     GKVHFGVKKEALLTMEIETWDPEECPLCKEGIPIVKPGSHK"
     gene            complement(2985..3908)
                     /locus_tag="VF531_04040"
     CDS             complement(2985..3908)
                     /locus_tag="VF531_04040"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX7713169.1"
                     /translation="MAQPSLAIRIGALELRNPLLPASGTYGYGREYLPFFSPDLFGAV
                     VSKGVSLKPWPGNRPPRIYETPAGMLNAIGLQNPGVEYFIKDALPFIREYHTPLIVNI
                     VGKTVAEYAEVAAILSPYPEVSGFELNISCPNVKEGGIAFGTDPKAAAAVTRAVRAET
                     AKPLIVKLSPNVTDITAVAREVEAAGADGLSLINTLIGMAIDIKKRRPVLGNLVGGLS
                     GPAIKPVALRMVWETYRQVKIPLIGIGGITSAADALEFIMAGATAVAVGTGIFRDPAI
                     PEEIIAGIGSFLVEEGISDIASIRGLAHQGF"
     gene            complement(3920..4711)
                     /locus_tag="VF531_04045"
     CDS             complement(3920..4711)
                     /locus_tag="VF531_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX7713170.1"
                     /translation="MQRTQGLIVDSRVLAPGYYQMTLLAPEIAATAGPGQFIEVRVAD
                     GTIQDPLLARPLSLFRINRKEGTISFIFKIVGRGTRLLATLKTGGTVVVWGPLGNHFN
                     PPGKETRIALVAGGVGMPPMFALAEFLREATGPDVPEIVLFYGGRTRCDLLELDSWNK
                     LGVTVHTATEDGSFGSQGVVTVALEEALKGHTYDYIASCGPIPMLRAVQQLAMRYRVP
                     GQMSLEAYMACGVGACLGCVCETRRGFLRVCSDGPVFPIEEVIFS"
     gene            complement(4764..7994)
                     /gene="carB"
                     /locus_tag="VF531_04050"
     CDS             complement(4764..7994)
                     /gene="carB"
                     /locus_tag="VF531_04050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7713171.1"
                     /translation="MPLDQTLKKVLVIGSGPIIIGQAAEFDYAGTQACRSLREEGISV
                     VLVNSNPATIMTDVNIADRVYVEPLTVESLRRIIAKERPDGLLPTLGGQVGLNLAVKL
                     SEAGVLSDYKVKLLGTPLEAIQKAEDRQLFKETMQALGEPLPESAIVGKLQEALDFAG
                     QVDYPLIIRPAYTLGGTGGGIAHDRAELEEITVRGLTLSPISQVLLEQSVAGFKEVEY
                     EVMRDSANNCITVCNMENIDPVGIHTGDSIVVAPSQTLADREYQMLRAASLRIIRALG
                     IEGGCNVQFALDPESFQYYVIEVNPRVSRSSALASKATGYPIAKVAAKIAIGLHLDEI
                     QNAVTGKTYASFEPTLDYVVVKIPRWPFDKFSLADRTLGTQMKATGEVMAIDRSLEAA
                     LMKAIRCLEIGQYGLALPGAEEMSDDLLLAALVHPDDRRFFFLAEALRRGYTIAELNR
                     RTKVDCFFLRKLDNLTAMEKEIKNRGFRDPEFLRKVKSMGFPDKEIARLAAVKEEEVR
                     EFRYTNGIRPVYKMVDTCAAEFEAATPYFYSCYDLENEAIPTGRRKIAVIGSGPIRIG
                     QGVEFDYCSVHSVFALKAAGVEAIIINNNPETVSTDFDTGDRLYFEPLTVEDVLHILE
                     LEKPEGVIVQFGGQTAINLAAKLEQAGIKILGTSVDSIDLAEERKRFDRLLLDLKIPK
                     PAGRTVVSLPEALEVAAEIGFPVLVRPSYVLGGRAMEIVYNQTELKNYLTSAVKAAPE
                     HPVLVDKYVSGRECEVDAISDGTGVLIPGIMEHVERAGVHSGDSFAVYPAQHLTVLEQ
                     EKILNYTVKLAKALKVVGLINIQFVLDEGEVYCIEVNPRSSRTVPFMSKVTGIPMVQV
                     ATRAVLGESLYEQGYAGGMWPETKLVAVKAPVFSFAKLTQVDISLGPEMKSTGEVMGV
                     DLSYFGALYKAFIAAGFTIPRGGTILATLADRDKAEALPLLKRLSERGFKIMATMGTA
                     AFLRVKGVPVTKLNKLDEGSPNIMDVIKNGEIQLLVNTITHGKEPERDGFKIRRAAVE
                     HNIPCLTSLDTVKAFLQVLLVLDEGDRSGLVLPLQEFKTIRVEQAI"
     gene            complement(8062..9126)
                     /gene="carA"
                     /locus_tag="VF531_04055"
     CDS             complement(8062..9126)
                     /gene="carA"
                     /locus_tag="VF531_04055"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7713172.1"
                     /translation="MTQARLVLEDGTVFYGESFGAAGEIGGEVVFNTGMTGYQEILTD
                     PSYSGQIITMTYPLIGNYGINRDDFESTHPFARGFIVREYCDTPSNWRSEMSLDDFLK
                     SHGLLGLAGIDTRALTKRLRTQGTMRGIITTSSEPDDAIRERVQKIQDLSGQDFVKMA
                     STDRIYCEGEGTKHVVLMDFGAKGNISRCLMKQGCRVTVVPCDTGSETIMKLKPDGVM
                     LSNGPGDPKDVPYAVQTVRELIGKLPIFGICLGHQIIGLALGGDTYKLKFGHRGGNHP
                     VKNLMTGEVTITSQNHGFAVRAESLPGDVLVSHINVNDRTVEGLCHRNLPLFSVQYHP
                     EAAPGPTDSEYLFAEFVGKL"
     gene            complement(9231..10523)
                     /locus_tag="VF531_04060"
     CDS             complement(9231..10523)
                     /locus_tag="VF531_04060"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7713173.1"
                     /translation="MKYCIKGGEIIDPAGGYLGRGDLLFCDGKVVAVGTNLAESSDAE
                     VIPATGKLVLPGLVDLHCHLREPGREDEETVASGARAAVQGGFTAVVAMANTDPAADN
                     AVVISYVHGQATAAGFAKVYPAGAITKGLKGEELAEMGAMAEAGAAAFSDDGKTVMNA
                     AVLRTAAEYSKLFDKPLLLHEEDRNLVGDGVMHEGYWATALGLPGIPSLAETAQIARD
                     LLIAEYTGARFHFCHLSAKESVALIRRAKECGLAVTAEVTPHHLCLTDAMLEGFDPVY
                     RVSPPLRSEADREALWEGLSDGTIDAIATDHAPHSLEEKHREFSLAPTGMTGLETALA
                     AVWTELVITGRISREKLVERMAVRPAQILKKTGGRLRPGDPADLMIFDPDCTWTVKPE
                     DLTSRSRNNPFVGRELRGRVERVWVDGVLKYVHQSFTE"
     gene            complement(10507..11448)
                     /locus_tag="VF531_04065"
     CDS             complement(10507..11448)
                     /locus_tag="VF531_04065"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7713174.1"
                     /translation="MIRLPKDLLGLQDLSCDQINLILETAMVCKDIFKRDLKKVPTMR
                     GKTVVTLFFEPSTRTRTSFEIAAKWMSADTINLTVSSSSVAKGESFVDTGRTLEAMGV
                     DVVVIRHSLAGTPKLLADSISAHVINAGDGAHEHPTQALLDLFTIREQKGSFTGLEVV
                     IVGDIMHSRVAKSNIWGLSKLGAKVTVVGPPTLIPPGLERMGVRVEHDLDRALATADV
                     VNILRIQLERQAKAYFPTLREYSRLYGMNPERLRRAKPGLMVMHPGPANLGVEISEAV
                     SVDPQSVITRQVTNGVAVRMALLYLLTGGGNKDEVLH"
     gene            complement(11527..>12584)
                     /locus_tag="VF531_04070"
     CDS             complement(11527..>12584)
                     /locus_tag="VF531_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX7713175.1"
                     /translation="APGNLGSPEKIAVAMSGCFAVGVVYLIVSLIIYKFGTGWIDVVL
                     PPVVVGSVVIVIGLALAKTAVDMAMNGSGTTPQPQFLLIAFVSLIIAILASAFFRGFL
                     GVIPILIGIIGGYIFSLFMGQVNFQAVSQAKWVAVPAFIFPKLNWGVVAAFAPVSLVV
                     ITEHIGHLIVTNNIVGRNFIKDPGLHRSLAGDGVATAVASLFGGPPNTTYGENIGVMA
                     ITRVFSVWVIGGAAVIAVLLSFIPKVGFFIRTIPTPVMGGICILLFGIIASAGIRMLV
                     EAGIDFSQKRNLIIASVILVLGIGGAKVTIAGVEFGGMALATYVGIILNLIIPHTKDL
                     EGGKGEKGNMPHPASTK"
BASE COUNT         2896 a         3733 c         3132 g         2823 t
ORIGIN      
        1 actctttccg gtcttttggg attcaagggt cagccgccgc accgcctcgt gggcatcggg
       61 atgaccaagg gaagccaata aaatatagag cggctcagcg atgatcattt ccttggtgag
      121 gtcaaaatta tgactcatcc gttcctcatc caccaccagc cgctctaaaa cccgggtcag
      181 ccgttgaacc gcctcggcga atcccaccac gatctccggg acaaaacgcc ccgatgccga
      241 attggtcaaa tcccgctgat gttccgatat ctgatcggag taaagggtga tcatgcgggg
      301 catgaactcc ttccacgtgc ttttgacatg ctcaaagttc cagggattcc gcttatgcgg
      361 catcgtggag gaacccacct gagtggcctc gaaagtctct cctacctctc ccagctcggt
      421 acgctgcaag ttccggatat catcggccag attggccagg actccgagag ctgaaatgat
      481 agtgtgaata tagtcggtca aatactcggg ggccacgatc tggctggagt aatcggccgg
      541 cttgaggccc aattccgcca atacttccgt ttcaaatccc attgggtctt ggaaaaaaag
      601 cgagctggca ttataagcac caaccgcacc cgaaatctta ccacgcaggt ttgatcccgc
      661 cgcttgaatc gccgtgatgc ggtcgccgaa acggcttaca tactcggcca tggcgaaacc
      721 gaaagtgatg ggtaccgcat gttgaccatg ggtccgtccc acctgtaaag tggctttttc
      781 ccgccgggcg atggtgatca atactcgttc cagcgccaga cttctaggga cgatcacttg
      841 agtggtggcg tccttgaagc ggagggccgt agcggaatcc atgatatcca ccgaggtggt
      901 tgtaaaatga acgaagggct tcgccgcgtc actcacccgg cgttgtacgc aattgaccag
      961 cgcccggatg ttatgtttgg tgatctcttc ttcccggtaa acttcctccg gggttacttc
     1021 ttcgcaggca gtggcaatct ctgcggcaac cgcctcggag caaagccccc gccgggccaa
     1081 aacccgggcc agagcggctt ccacttttaa ctcgtaacgg atataagcat tttcagaaaa
     1141 aaaggccccc agctcagtga aaagcgcttc attggaagcg taatacctgt gatcgagcgg
     1201 actaagattc tcgaaaacat cccgttccaa atcatacccc tcccaaataa aaaagggcgg
     1261 acaggttgaa tgcgtctcgc atttgaaacc tatccgcccg ggtttttatc cctctacggt
     1321 gtaacgccgt cccccacaca tgtgtatggg ctacggacgc ctgcatcact tggaccggac
     1381 ctttaggaac ccccactaaa aaaacggggg gtttaaagcg gaaccctagg tgcacttcca
     1441 taaaatattc tctttttcaa tgtttttatt ctaacaaaac cccgccccgg ttgtcaatct
     1501 tcaaaaacac gggctcacgg ccggaaccca agtccacatc ggattcgggt atgaatctaa
     1561 aattttgata caatttgtat aaaaattagt aaaccttctt tcatccctca atacggcctg
     1621 gtggcaatta cagcacagtt gtcggcctgg tagaccagtt cgcaaccggt gaaacccgcc
     1681 tacgcgcgcg cgcaactact tccccgtttt atggcccaaa tattcccgat aaccgatctg
     1741 ttcaagcata gcggccttac cctcgacatc ctcggctaat tcatgtttat attcaatgag
     1801 cttccggcgt aattggggat cggccactcc cacgatctga actgccaaca ccccggcgtt
     1861 ttttgccccg ttgatgctca ctgtggctac cgggataccg ctgggcatct ggacaatcga
     1921 atatagcgag tctactccgc caagggcggc ggtttggatc ggaattccga tcaccggtaa
     1981 gggtgtcaag gcagccaaca caccgggcag gtgtgctgcg ccgccagccc cggcgatgat
     2041 tacttccagt ccccggtcta ccgccgatga accgtattca accgcttttt ggggtgaacg
     2101 gtgcgcggag atgatggtca tttcgaaatc aattccgaaa tgttccagga tctccgcggt
     2161 ttgtttcagg ataggcagat cagagtcgct gcccatgaca attccaaccc gcggcatcgt
     2221 gcacctcctt ttactaactc atggttaccg tcgcgcggtg cttttcacaa ctcgtttatg
     2281 cgcgtacgcg cgcaatttcc ggctttagcc ggctggtacg tggtttataa acggatttat
     2341 ttatgacttc ccggtttgac gatgggaatt ccttccttgc aaagaggaca ttcctccgga
     2401 tcccaggtct cgatctccat cgtcaataga gcctctttct tcacaccaaa atgtactttt
     2461 ccgccgctgc ggtccaccag gatcccaacc ccgcagacat tggcgccccg ctccttgacg
     2521 gcttccatga cctccagtac tgaaccaccg gtggtgatca catcctcgac gatcaacaca
     2581 ttttcatcat catccagttc aaaccctcgc cggaaagtca tcttcccgtc ttccctttcc
     2641 gtaaaaagcg cccttactcc aagaactttt gccacctcat atgcgataat gattccaccg
     2701 atagccgggg ccactacggc atgcaactcc tgccccttga aacgccgggc caaatcttca
     2761 cagagtctct cggtaaattt gggatattgg agtacctggg cacattgcat atactgccgg
     2821 ctgtgccggc cggaagtcaa ccggaaatgc ccgtcccgca agactttcgt ctcacggaaa
     2881 atacccaaaa tttctttctg atctaacaca gaggtctccc ccttacagta taataacctc
     2941 atggtatagt ggctgcctgg tctcaccgat ttaggtatcc atactcaaaa tccttgatgg
     3001 gcgaggccac gaatactggc tatatccgaa attccttctt cgacaagaaa acttccgatt
     3061 cctgcaataa tctcctccgg aatcgcagga tctctaaaaa tcccggtacc aaccgcgacc
     3121 gctgtcgctc cggccatgat gaattcaagg gcatccgcgg cactggtgat gccgccgatc
     3181 cctatcaacg gaattttcac ttgtcggtat gtctcccata ccatccgcag tgctacaggc
     3241 tttatggcag gaccggatag accgcccacc agattgccca gtaccggccg gcgttttttg
     3301 atatcaattg ccatgccgat cagcgtgtta atcaggctca ggccatccgc acctgccgcc
     3361 tccacctctc tggcaacggc ggtgatatcg gtgacgttcg gcgagagctt gacaatgagc
     3421 ggcttggctg tttctgcccg caccgcccgg gtgaccgccg cggcggcctt cggatcggtt
     3481 ccgaaagcta tcccgccttc cttcacattg ggacaggaga tattcaactc aaaacccgaa
     3541 acctcggggt acgggcttaa aatcgccgcg acctcggcgt actccgctac ggtcttaccc
     3601 acaatattga cgatgagcgg cgtatgatac tcccggatga acggaagcgc atcctttata
     3661 aagtattcga cccccggatt ttgtaatccg attgcattca acattcccgc cggggtctca
     3721 taaatccgcg gcggccggtt tccgggccag ggctttaagg ataccccctt ggagacgacc
     3781 gctccgaaaa gatccggaga aaaaaagggc agatactccc gaccatagcc gtaagttccg
     3841 gaagccggaa gcaatggatt gcgtaactca agcgccccga tgcggatcgc cagggacggc
     3901 tgagctattg aaggctgatt catgagaaga tcacctcttc gatgggaaag accggcccgt
     3961 cgctgcaaac ccggagaaaa ccgcgccgag tttcgcaaac gcaacccaga caggctccta
     4021 caccgcacgc catataagcc tccagcgaca tttggcctgg aacccggtag cgcattgcca
     4081 gttgctgcac agcccggagc atcggaatcg gcccgcagga agcgatgtaa tcgtatgtat
     4141 gacccttgag ggcctcttcc agggccacgg taactacccc ttggctccca aagctcccgt
     4201 cctcggtagc ggtatgaacc gttaccccca gcttgttcca actgtccaat tccagaagat
     4261 cgcatcgggt ccggccgccg taaaataaaa cgatctccgg gacgtccggt ccggttgcct
     4321 ccctcaagaa ctcagccagg gcaaacatag gcggcattcc cactcccccg gccaccaggg
     4381 caatccgggt ttcctttcca gggggattaa aatggttacc gagcggcccc cagactacaa
     4441 ccgtcccgcc agtttttaga gtagctaaaa gccgggtgcc gcggccgacg attttaaaga
     4501 taaagctgat ggttccctct ttccggttga tccggaaaag actgagtggc cgggccaaca
     4561 gcggatcctg tatggtgcca tcggccaccc gaacctcgat gaactgcccc ggcccggcag
     4621 ttgcggcaat ctccggggca agtagcgtca tttgataata ccccggggcc aaaacccggg
     4681 aatccacgat taatccttgg gttctttgca tcaaccatcc atccgtattc gtctattctc
     4741 aatatcaggc ataccggatc aatttagatc gcctgttcca ccctgatcgt tttgaactcc
     4801 tgaagcggca ggaccaaacc ggatctatcc ccctcatcca ggacaagcaa gacttgaagg
     4861 aatgctttca ccgtatccag cgaagtcagg caagggatat tatgctccac tgcggcacgg
     4921 cgtatcttga acccgtcccg ttccggctcc ttgccgtgag tgatagtatt caccaacagc
     4981 tgtatttcac cgttcttaat gacatccatg atatttggcg atccctcatc caacttgttg
     5041 agcttggtaa ctggaactcc cttgacccgg aggaaagcgg cagtccccat ggtagccatg
     5101 atcttgaagc cacgttccga gagccgctta agcagcggta aagcctccgc cttatcccgg
     5161 tccgccagtg tcgccagaat cgtaccaccc cgtggtatgg tgaacccggc ggcaataaag
     5221 gctttgtaaa gtgcgccgaa atacgaaaga tccactccca tcacttcacc ggtggatttc
     5281 atctccgggc ctagcgaaat atcgacctgg gtgagcttgg caaaggaaaa aaccggagcc
     5341 ttcacggcta ccagtttggt ttccggccac atccccccgg cataaccctg ttcatatagg
     5401 gattccccca acacagcgcg ggtagcgact tgtaccatag ggatccctgt gaccttgctc
     5461 ataaagggca cggtccggct ggagcgggga tttacctcga tgcaatagac ctcaccctcg
     5521 tccaggacaa actgaatgtt gatcagtccg accactttaa gtgctttggc tagcttaacc
     5581 gtatagttca agattttttc ctgctccagg acggtgagat gctgagccgg ataaacggcg
     5641 aaggagtctc ccgaatggac cccggcccgt tccacatgct ccatgatccc gggaatcaga
     5701 acgccggtac cgtcggagat ggcgtctacc tcgcattccc ggccgctgac atacttgtcg
     5761 accagtaccg gatgttcggg ggcggccttg accgcagaag tcaggtaatt cttaagctcc
     5821 gtctggttat agacaatctc cattgctcgg ccgcccagga cgtaagaggg acggaccagc
     5881 accggaaatc cgatctccgc cgccacttcc aaagcctcgg ggagcgaaac aacagtccgg
     5941 ccggcaggtt tgggaatctt cagatccaac aagagtctat caaaacgttt tctttcctcg
     6001 gccaagtcaa tggagtccac cgaagtgcca aggatcttga ttcccgcctg ttccaacttg
     6061 gcagcgaggt taatggcggt ctgtccgccg aattgaacaa tgactccctc gggcttttca
     6121 agctccaaaa tatggagcac atcctcaacg gtcaagggtt cgaagtaaag ccggtcaccg
     6181 gtgtcgaaat cggtactgac cgtctccgga ttattattga taatgattgc ctcgactcct
     6241 gcagccttta gggcgaaaac cgaatggaca ctacaatagt cgaattcgac cccctgaccg
     6301 atccggatgg gccccgagcc gataaccgcg atcttccgtc ggccggtggg aatcgcctca
     6361 ttctccagat cgtaacatga gtaaaaatat ggggtggcag cttcaaactc tgcggcacaa
     6421 gtatcgacca tcttatagac cggccgaatc ccgtttgtat aacggaattc acggacttct
     6481 tcttccttga ccgccgccag ccgggcaatc tccttgtccg ggaaacccat ggattttacc
     6541 tttcgcagaa actccgggtc acggaaaccc cggtttttaa tctctttctc catggctgtc
     6601 aaattgtcca atttgcgcag gaagaaacag tctaccttag tccgccggtt cagttcggcg
     6661 attgtataac cgcggcgcaa ggcttcggcc aaaaagaaga acctgcggtc gtcgggatgt
     6721 accagcgccg ccaggaggag atcgtcgctc atctcctccg ctccgggaag cgccagaccg
     6781 tactggccaa tctccagaca acggatggct ttcatcaagg cggcctccag ggaacggtcg
     6841 atggccatta cttcaccggt agctttcatc tgagttccca gcgtgcgatc ggccagactg
     6901 aacttatcaa aaggccagcg ggggatcttc accaccacgt aatccaaggt gggttcgaaa
     6961 gaggcgtatg tctttccggt cacggcgttt tggatctcat ccaggtgcag cccgatggcg
     7021 atcttcgccg ccactttggc tatggggtat ccggtggcct ttgatgccag ggcgctggaa
     7081 cggctaaccc tgggattaac ctcaatcaca taatattgga agctctccgg gtccagggcg
     7141 aactggacat tgcagcctcc ttcgattccc aaggcccgga tgatccggag cgatgctgcg
     7201 cgcaacatct ggtattcccg atcggccagt gtctggctgg gagccacaac gatgctgtcc
     7261 ccggtatgaa tccccaccgg atcgatattc tccatgttgc atacggtgat gcaattattg
     7321 gccgagtcac gcatcacctc gtattcaact tctttgaacc ctgccacaga ttgctccaaa
     7381 agcacctggg agatggggct caaggtcagt ccccggacgg ttatctcctc gagttccgcc
     7441 cgatcatggg cgataccgcc gcctgtaccg cctaaagtgt aggcaggccg gataatcaac
     7501 ggataatcca cctggccggc aaagtccagg gcttcctgga gcttccctac aattgcgctt
     7561 tcaggtagcg gttctcctaa agcttgcatg gtctctttaa aaagttggcg atcctccgct
     7621 ttttggatgg cctcaagggg ggtgcccagc agtttcactt tataatcgct caacacgccc
     7681 gcctcggaga gcttcaccgc cagatttagc ccgacctgcc cgcccagggt gggcaaaagg
     7741 ccatcaggtc gttccttggc gatgatccgg cgcaatgact ccacagttag gggttcaacg
     7801 taaaccctgt cggcaatatt gacatccgtc atgatcgtag ccggattgga gttaaccagc
     7861 accaccgaga taccttcctc ccgcagcgag cggcaggcct gggtaccggc ataatcgaac
     7921 tccgccgcct ggccgatgat gattggtccc gacccgatga caagcacttt ctttaatgtt
     7981 tggtccaaag gcatcttttt caccactact taaatttttg gattcaatga cttgtatata
     8041 tattaattta ccgaattacg gctagagctt accgacaaac tcagcgaata aatattcgga
     8101 atccgtagga cctggagccg cctcgggatg atactgcacc gagaaaaggg gcagattcct
     8161 atggcataat ccctccacgg tccgatcatt gacattgata tgagagacca aaacatctcc
     8221 gggcaacgac tcggcgcgta ccgcgaagcc gtgattctgt gaggtgatag tgacctctcc
     8281 ggtcattaga ttcttcaccg ggtgatttcc gccacggtgg ccgaatttca gcttgtaggt
     8341 atcgccaccc aaagccaggc cgatgatctg atgacccagg caaatgccga aaatcggcaa
     8401 ctttccgatg agctcccgga ctgtttgtac ggcgtagggc acatccttgg gatcccccgg
     8461 tccgttggag agcatgaccc cgtcgggttt tagtttcatg attgtctcgc tcccggtatc
     8521 gcagggaacc accgtcaccc ggcaaccttg cttcatcagg cagcggctga tattcccctt
     8581 ggctccgaaa tccatcaata caacgtgttt ggtaccctca ccctcacagt aaatccggtc
     8641 ggtggaagcc attttgacga agtcctggcc ggataggtcc tgtatctttt ggacccgctc
     8701 ccggatggcg tcatccggtt cggatgaagt ggtaatgatc ccgcgcatgg taccctgggt
     8761 ccgaagccgt ttcgtcagcg cccgggtatc gattcctgcc agacccaaca gtccgtggct
     8821 cttcaggaaa tcgtccagac tcatctccga ccgccaattg ctaggggtgt cacaatactc
     8881 ccggacaatg aagcctctgg cgaaggggtg ggttgattcg aaatcgtccc tgttaatccc
     8941 gtagttgccg atgaggggat aggtcatggt gatgatctgg ccgctgtaag agggatcggt
     9001 taaaatctct tgataaccgg tcattccggt attaaaaacc acttcgcctc cgatttctcc
     9061 tgcggcgcca aaggattcac cataaaaaac cgttccgtct tccaggacca aacgggcttg
     9121 cgtcattcac aaccgacctc ccagagcgta tatattaaag cgaaaccgtt gccgatcccg
     9181 gtaaaggttt tcaaactatt atatcataaa tattcattaa agcttgatat ttattcagta
     9241 aaactttgat gaacatattt tagcaccccg tcgacccaga cccgttccac cctaccccga
     9301 agctcacggc ctacaaaagg gttattccgg gaacgtgatg tcagatcctc cggcttaacc
     9361 gtccatgtac agtcggggtc aaagatcatc aggtccgcag ggtctcccgg ccgtaacctg
     9421 ccgccggttt ttttcaaaat ctgggcggga cggactgcca tcctttccac cagtttttcc
     9481 ctggatatcc gtccggtaat caccagttcg gtccagactg cggcgagggc tgtctccaga
     9541 ccggtcattc cggtaggggc gagtgaaaat tcacggtgct tttcttccag ggaatgaggc
     9601 gcatggtcgg tggcaatggc atcgatggtg ccgtcggaca acccttccca aagcgcttcc
     9661 cgatcggcct cgctccgtag cggcggactc acccggtaaa ccgggtcaaa tccttccagc
     9721 atcgcgtcgg tcaggcagag atgatgcggg gttacctcgg ccgtcaccgc caaaccgcac
     9781 tccttggcgc ggcgaatcaa tgccacgctt tctttggcgc ttaaatgaca aaaatggaac
     9841 cttgccccgg tatattcggc gataagcaga tcgcgagcga tctgcgccgt ttcggcaagc
     9901 gaaggtattc ccggcagtcc cagcgcggta gcccaatatc cctcatgcat cacgccgtca
     9961 ccgaccagat tccgatcttc ctcatgcagg agcagcggct tgtcaaaaag cttcgaatac
    10021 tctgccgctg tccgtaacac cgcggcgttc atcacggtct taccgtcatc ggagaaggct
    10081 gccgcgcctg cttcggccat ggctcccatc tccgccagtt cctcgccttt taagcccttg
    10141 gtgattgccc cggccggata aaccttggca aagccggccg cggtcgcctg accatgtaca
    10201 tagctgataa ccacggcgtt gtcggccgcc ggatcggtgt tggccatggc taccaccgcc
    10261 gtgaaaccac cttgtaccgc cgccctggcg ccactggcta ccgtttcctc atcttcacgg
    10321 ccgggttcac gcagatggca atggagatcg acgagtcccg gcaataccaa ttttccggtc
    10381 gcagggatca cctccgcgtc ggaggattcg gccagattcg ttcccacggc taccaccttg
    10441 ccgtcacaaa acaacaagtc gccccggccc agatacccgc cggccggatc gatgatctcc
    10501 ccgcctttaa tgcaatactt catctttatt gcctcctccg gtcaacaagt acaacaaagc
    10561 catccggacc gccactccgt tcgtaacctg ccgggtgata accgattggg ggtcgactga
    10621 aaccgcctca ctaatctcca cccccaggtt ggcagggccg ggatgcatca ccatcagccc
    10681 gggcttggcc cggcgtaacc gttccggatt catcccgtat aaccgggaat actcgcggag
    10741 ggtcggaaag taggccttgg cctgccgctc caattgaatg cggagaatat tcacgacatc
    10801 cgcggtggca agcgcccgat ccagatcatg ttcaacccgg acccccattc gctccaggcc
    10861 cggaggaatt aaggtcggag gtccaaccac agtcaccttg gcccccagtt tcgacaaacc
    10921 ccagatgttg gatttggcaa cccggctgtg catgatatca ccgacgatga ctacctccag
    10981 tccggtaaag ctcccttttt gctcccggat ggtgaacaga tccagcaatg cctgggtagg
    11041 atgttcatgt gccccgtcgc cggcgttgat cacgtgagcc gagatggaat ccgccaaaag
    11101 tttcggggtt ccggccaatg aatgccggat caccaccaca tccacaccca ttgcctccaa
    11161 agtacggccg gtgtcgacaa aactctcgcc cttggccaca ctgctggaag agaccgtgag
    11221 attgatggta tcggcactca tccatttggc ggcgatctca aaggaggtcc gtgttctggt
    11281 actgggctca aagaagagcg tcactaccgt ttttccacgc atggtcggta cttttttcaa
    11341 gtcgcgttta aaaatgtcct tacaaaccat agcggtttcc aaaatcaggt taatctggtc
    11401 gcagctcaag tcctgcaatc ccagtagatc tttgggcagc cttatcatat aactcgctcc
    11461 cgtttagtaa tctctatatt ctaaggagtg cgccgatgcg cactccttag cgcaaatata
    11521 tcagtgttat ttcgtcgacg ccgggtgggg catatttccc ttttcaccct tgccgccttc
    11581 caaatccttg gtatgcggga tgatgaggtt gaggatgatc cccacataag tggcaagtgc
    11641 cattcctccg aactccaccc cggcgatggt cactttagcg ccaccaatcc ccagaaccag
    11701 gatgaccgag gcaatgatca gattccgctt ctgcgaaaag tcgatccccg cctcaaccag
    11761 catccggatg ccggcggatg cgatgatccc aaaaagcagg atacagattc cgcccatgac
    11821 cggagtgggg attgtccgga tgaaaaatcc gactttgggg atgaaggaga gtagtaccgc
    11881 gataactgcg gccccgccga tgacccagac gctaaaaaca cgggtgatgg ccataacccc
    11941 gatgttctca ccataggtgg tattgggcgg accgccaaaa agactggcta ctgccgtcgc
    12001 cacgccgtcc cctgccagtg aacggtggag acccggatcc ttgatgaagt tccgtcccac
    12061 gatattgttg gtgacgatca ggtgcccgat atgttcggtg atgaccacca gcgaaacggg
    12121 ggcgaaagcc gccaccacac cccaatttaa tttagggaag atgaacgcgg gaactgcaac
    12181 ccatttggct tgggaaaccg cctggaagtt gacctggccc ataaagagcg aaaaaatgta
    12241 tccgccgatg ataccgatga gaatggggat tacacccaaa aagccccgga aaaaagcaga
    12301 tgccagaatg gcaataatca atgaaacaaa ggcaataagg agaaattgtg gttgcggagt
    12361 cgttccgctg ccgttcatcg ccatatccac cgcggttttg gctagcgcca aaccgataac
    12421 gataaccacc gaacccacca ccaccggcgg gagcactacg tcaatccagc ccgttccgaa
    12481 tttataaatg atgagtgaaa cgataaggta aaccactccc actgcgaaac agccgctcat
    12541 cgcaacggcg atcttctcag ggcttcccaa attgcccgga gccg
//
LOCUS       DASVMB010000127         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_309913,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000127 DASVMB010000000
VERSION     DASVMB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309913"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VF531_04075"
     CDS             complement(<1..505)
                     /locus_tag="VF531_04075"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX7713176.1"
                     /translation="MAGNDYINHSYMIKHGPYKDFECTIRKVQTNPVVKIYADIQFIR
                     KTVEIELKPEDLGLPIEVELSQYPSNIVETWINNCINHNQMKQFLNYGASEADIDSFK
                     SKLNIEVPDCFYQFYRNFNGMRYSKLDEISDNGYFLYDQSSAILNLNDVLETKRSWDV
                     FIRDMRLN"
     gene            complement(520..1026)
                     /locus_tag="VF531_04080"
     CDS             complement(520..1026)
                     /locus_tag="VF531_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713177.1"
                     /translation="MDEKRLNEAMEGIPQAWACALIGEIDFAIEMLNRSLEVVDEFFM
                     EQKLDLDGIFKYLEGLKLRGDLYQKKGDFEGYFNASNSYATATGIYRALREQGRLPEE
                     DKRQYFLLLAKRARMYRKHDNPQAALESYAEIFNGMTKDEISEYADRMDELDELVQRT
                     WGESKEGA"
     gene            complement(1081..2079)
                     /locus_tag="VF531_04085"
     CDS             complement(1081..2079)
                     /locus_tag="VF531_04085"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX7713178.1"
                     /translation="MKIRMEKRDEKPAKFWEIEVRGKMVTVRFGRSDGGVQTQTEDEF
                     SSQEAANHEAEQQIDEKFKQGYKVAADDERVEDTSAQTTAQAEAIIAKIKAALNEDSL
                     PYIGLEIETFHDEKPEPWQSNIGIQAYWPLEMETLKDSNGRELSFFGQMNLEELPKLP
                     GYPEKGILLFFLSREHSDFEEEARVLYFDTVCHDASRLKSQSGLSFVTEGAYRITAAP
                     EIQASHPYPPDRRFDQIIEQHGTKDKKVMELVEEEIAEWEHENEGHRMGGYCFFTQED
                     PRGRDEYSDYVNLFQIDELDDEIMLWGDSGIAHFLIHPDDLAKGDFSKVLFVWDCY"
     gene            complement(2474..2779)
                     /locus_tag="VF531_04090"
     CDS             complement(2474..2779)
                     /locus_tag="VF531_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX7713179.1"
                     /translation="MRFWILVVGMAAVTYVPRLLPLVTLTERPLPPLLRRILLYIPYT
                     ALGALIIPGVWQAIPGRPEAAMAGIGAAAICSWFKGSLIVAVIAAVGVAWVVLQFIP"
     gene            complement(2776..3492)
                     /locus_tag="VF531_04095"
     CDS             complement(2776..3492)
                     /locus_tag="VF531_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX7713180.1"
                     /translation="MNLKKAFSMSAVTAGLKAGFPIAVGYLPVAMAFGFLSKTVAVSV
                     VDSFLCSLLVFAGASQFMALNLWRIGVAGGEIILATLLLNLRHLLMSATVAARLESRV
                     NRWLPLVAFGVTDETFAVAATRPEPLTVSYLLGLEGIAYSSWVGGTVLGYLLGSVLPE
                     AIQGSMGIALYALFAAILVPQVKHSFKAAVLALSSGLINLFLSGSRLLPGGWSMVLAI
                     IVTAIAGGLLFREETGEVRS"
     gene            3715..>4398
                     /locus_tag="VF531_04100"
     CDS             3715..>4398
                     /locus_tag="VF531_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713181.1"
                     /translation="MSRYHADHRLTLILMILIVGLLNADQNIINATLGPIEKEFQVDD
                     AAIGLMSGLFTLLGAVISLIWGYWSDKSNRKNLFTISVLIGQIPCLLTAFAANYQQFF
                     ILRILTGIGVGASFPTIFSMLGDMYSEKERAVAVTWMTTVMGIGQIAGELLGGYLSPS
                     FGWRLPFILVAVPNLLILVIFHLIVAEPKRGMREEAFEELVDAGFVYPKTIKLRDYLN
                     LIKVRTNIYL"
BASE COUNT         1190 a         1171 c          843 g         1194 t
ORIGIN      
        1 aattgagcct catatcacgt ataaaaacat cccaagatct tttagtttcc aaaacatcat
       61 tcaaatttaa aatagcgctt gattgatcgt acaaaaaata gccgttatca cttatttcat
      121 ccaatttgga atatcgcatt ccattaaaat tgcggtaaaa ttgataaaaa caatcgggta
      181 cttcaatatt taattttgac ttaaatgaat cgatatcagc ttctgatgcc ccgtaattta
      241 gaaattgttt catttggttg tgattgatac aattattgat ccatgtttca acaatattcg
      301 acggatattg agaaagttca acctcgatag gtagacccaa atcttctggc tttaattcga
      361 tttcaacggt ttttctaata aattgaatat cagcgtatat ttttaccacc ggattggttt
      421 gtacttttcg gatggtacat tcaaagtcct tatagggccc atgttttatc atataagaat
      481 ggttaatata atcgtttcct gccatgtatg gacctcccat taggcccctt ccttcgattc
      541 tccccatgtc ctctgcacca gttcgtccaa ttcatccatc ctgtcggcat actcacttat
      601 ctcatctttg gtcataccgt taaagatctc agcgtaactt tccagagcag cttggggatt
      661 gtcatgttta cgatacatcc gggcccgttt tgccaaaaga aggaagtatt gtctcttgtc
      721 ctcctccggc agccggcctt gttccctcaa cgctcgatat atccccgtcg ctgtagcgta
      781 actattggag gcattaaaat aaccctcaaa atcacctttt ttttgatata aatcccctct
      841 aagcttaagg ccttccaaat acttgaatat accatccaaa tctaacttct gctccataaa
      901 aaactcatcc actacttcta aagatcgatt aagcatttca atggcaaaat caatctcacc
      961 aatcagagca caggcccaag cttgaggaat accttccatt gcctcattta gacgtttttc
     1021 atccattttc catcctcccc tttctttgaa acaaatggct caatctcgtt tgatcaaaca
     1081 ttaataacaa tcccaaacaa acaatacttt tgaaaaatca cccttggcca ggtcatccgg
     1141 atgaatcaag aaatgagcga tcccggagtc gccccaaagc atgatctcat catccagttc
     1201 atcaatttga aacagattta cataatcact gtattcatca cggccgcggg gatcttcctg
     1261 agtaaaaaaa caataaccgc ccatccggtg cccttcattt tcatgttccc actcggcaat
     1321 ttcttcctca accaattcca tgaccttttt atccttcgtg ccgtgttgct ctataatttg
     1381 gtcaaaacgg cggtccggcg gataaggatg gcttgcctga atctccggcg cagcagtgat
     1441 tcgataagca ccttcggtta caaagctgag tccggattga ctttttaacc tggaagcgtc
     1501 atggcagaca gtgtcaaaat aaagcacccg ggcttcttct tcaaagtcgg aatgctcccg
     1561 tgacaggaag aatagtaaaa tgcccttttc aggataaccg ggtaatttag gcaattcctc
     1621 cagattcatc tggccgaaga atgacaactc cctgccgttg ctatctttaa gtgtctccat
     1681 ttcaagcggc caataagctt gaatcccgat attactctgc cacggttccg gcttttcatc
     1741 gtgaaacgtt tcaatttcca aaccaatata cggaagggaa tcctcattca gtgcggcttt
     1801 tatcttcgcg atgattgctt cggcctgcgc tgtagtttga gcggaggtat cttcgactct
     1861 ttcatcatcc gcagccacct tgtacccttg cttaaacttc tcatcaatct gttgctccgc
     1921 ctcatgattc gccgcttcct gcgaagaaaa ctcatcctcg gtctgagtct gtacacctcc
     1981 gtcgctcctg ccgaaacgga ctgtaaccat cttgccccga acctcgatct cccagaactt
     2041 ggctggcttt tcatcccgtt tttccatacg gattttcatc gatattcccc tccaattcct
     2101 ttggtaactc tattgtggga agatttaaac agtctctttt tcccgcccgt gacaaaagta
     2161 tataaagtct caatcatcgc tctgaaaccg ctccaatctc tgaccttgag atggttttct
     2221 actgttagac attctccctg caatatccga aacgcttatc ttatatccgc taatacagtg
     2281 caaattctcc atatttaata tttttaatga atttattctt gtcttccata atataaagtg
     2341 gaaccatctt tgtcaataat acataacaat gatgcatctt atgttcacac aaattcacat
     2401 cccgtaccaa aacaaaaaga gccaagcaaa attccttgac tcttccatac gacagttcat
     2461 ttattcgtta ttttcagggt ataaattgca acaccaccca ggcaaccccc accgcggcga
     2521 taaccgcaac gatcaaactc cccttgaacc acgaacagat cgccgccgca ccgataccgg
     2581 ccatcgccgc ttcaggccgg ccaggaatgg cttgccatac tccggggatg atcaaggcgc
     2641 ccaatgcggt atagggaatg tacaacagaa tacgccgcaa cagcggcggc aatggccgtt
     2701 ccgtcaatgt aaccagtggt aacagccgcg gcacataagt tactgccgcc atgccgacaa
     2761 ctaaaatcca aaacctcatg accgcacctc cccggtttct tcccggaaca gtaagccacc
     2821 ggctatggca gtgacgatta ttgccagcac catggaccaa ccacccggta ataagcggct
     2881 tccgctcaaa aataaattga tcaacccgga actcagggcc aaaaccgccg ccttgaaaga
     2941 gtgcttaacc tgtggcacta aaatggcagc gaataacgca tacaaagcta tccccatact
     3001 gccttggatg gcctcgggca agacggaacc aagcagataa ccaaggactg tcccgccgac
     3061 ccaggaggag taggcgattc cttccaagcc caaaaggtat gaaactgtca acggttccgg
     3121 cctggttgcg gccaccgcga aagtctcatc ggtgaccccg aaggccacga ggggcaacca
     3181 acggtttacc ctgctttcca ggcgcgctgc gaccgtggcg ctcatcaaaa gatggcgtag
     3241 gttcaaaagg agcgtggcaa gtatgatctc cccacccgcc actccaatcc gccagagatt
     3301 caatgccata aactgactgg ctccggcaaa gaccaataac gaacagagga aactatccac
     3361 cacggaaact gcaaccgttt tagataaaaa accgaacgcc atcgctaccg ggaggtaacc
     3421 gacggcaatg ggaaaccctg ccttcaaacc ggcagttacc gcagacatgg aaaacgcctt
     3481 cttgagattc accgattctc acccattcat gaaagattca gattcgtatt ttatttcatt
     3541 tatctacatc tttatcaggt ccatattcgc gtttgaaccg tctttacctg caggatgcgt
     3601 taacttgaag atgattttcc aacgattgac acaggatgcc gagaaacgta taattgatac
     3661 agtattttgt attttatatc gatacataca cataaaaagc ttaacggagg aaacatgtcc
     3721 cgctatcacg cagatcatcg ccttaccctg atcctgatga tcctcatcgt aggcctgctc
     3781 aatgccgatc aaaacattat caacgccact ctggggccga tcgaaaaaga gtttcaggtg
     3841 gatgatgcag ccattgggct gatgagcggc ttatttacct tgttgggcgc ggttatcagc
     3901 ttgatttggg gatactggtc cgacaagagc aaccgcaaaa acctcttcac tatttcagtg
     3961 ttgatcggac aaatcccctg tttgctgaca gccttcgccg ccaactatca gcagtttttc
     4021 atcttgagga tcctgaccgg cattggtgtc ggcgcctcgt tccccaccat cttttccatg
     4081 cttggtgaca tgtacagcga aaaagaacgg gccgttgccg tcacctggat gaccactgtc
     4141 atgggaatcg gacagattgc cggcgaactc ctggggggtt acctaagtcc cagttttggc
     4201 tggcgtctcc cctttatttt agtggcagtt cccaatctgc tcatcctggt catctttcac
     4261 ctgatcgttg ccgagcccaa gcgggggatg cgtgaagaag ccttcgagga actggttgac
     4321 gccggctttg tataccctaa aaccattaaa cttcgtgatt acctcaatct gatcaaagta
     4381 cggaccaata tatacctt
//
LOCUS       DASVMB010000128         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_16404,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000128 DASVMB010000000
VERSION     DASVMB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16404"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VF531_04105"
     CDS             complement(<1..1013)
                     /locus_tag="VF531_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX7713182.1"
                     /translation="MKHETKIGHREALTLLVFIMSGKVFLQFPRDMASLGDGSGWIIV
                     ILTGILALVGFSFLYALIRRFPSQDIIEICQTLTGNFIGTGAGVLFFLFFLITTSLFL
                     RKFAESFILAILPQTPISVITAVFLILLMYGVFLGIETLSRVAWLYGPYLLGTLIIIL
                     VFSLPNGSVLSLTPVLGKGPGSILKNSLTHVSMFSEILLLGLIAPLIRDKKKIFGVGF
                     YSMGIAIIINTLVTMVVIFVFNYAGASEIIFPIFQLTRLITYGEFIQRVESIFVFLWF
                     FAAGIELSGLFYGTVVGFAETFRIRNYRPLIIPIAVICFTISLIPPSMTDTIILNDFV
                     LSK"
     gene            complement(1037..>2611)
                     /locus_tag="VF531_04110"
     CDS             complement(1037..>2611)
                     /locus_tag="VF531_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7713183.1"
                     /translation="KEPEGAESLSKSLAKNRARLEEIFEIPLNKDVVIREFKIFTGHL
                     NAILIYIDGLTDRNTQNFAILQPLMILEEKAGTDGEPINLIYERLLPENQVVKGRKFQ
                     EIIDNVLDGSSALLIEGSAQALFIETKGWEHRGIEKPSTEHVVRGPQEGFTETFRSNT
                     ASVRRYIKDPKLITEIFRVGKRSNTLVGFMYIKDIANPKLVEEVKYRIQSVSRVTDYI
                     AETGTLEEYLQDHPHSLIPQMLSTERPDRMGAHLREGFVGVVMANSPYALVIPTTFTI
                     FLHAAEDYYLRWPFGNFLRFLRAVAIFSALLLPAFYIAVINYHQEMIPTDLLLAMTAA
                     RESVPFPSIIEILFMELSFELIREAGIRIPSVIGPTIGIVGALILGQAAVTASIVSPI
                     LIIVIAITALSSFVIPNYNASFTIRLLRFIFVMFAGFLGFYGIAFGIFLLTLHMASLT
                     NFGVPFLSPISPYQPENKDRVIRPLNFNQPYRPVFLRPLDWIRQKDTARPWDRPDAVE
                     KEETHPEGGDSSSDQS"
BASE COUNT          729 a          614 c          700 g          568 t
ORIGIN      
        1 attttgctga gaacaaaatc attcagaata atggtatcag tcatcgaagg cgggatcaga
       61 ctgatggtga aacagatcac cgcaatcggg ataattaagg gccggtagtt gcggatccgg
      121 aaggtttcgg cgaaaccgac cacagtgcca tagaataatc cgctcaattc aatcccggcc
      181 gcaaagaacc agagaaagac gaagatcgac tccacccgtt ggatgaattc accgtaagta
      241 attaaccgcg tgagttgaaa gatggggaaa atgatttccg aagcaccggc atagttaaag
      301 acgaagatta ccaccatggt taccaaagta ttgatgataa tggcaattcc catgctgtaa
      361 aaaccaacac caaaaatctt ttttttatcc cggatgagcg gcgcgatcag acccaacaat
      421 aagatctcgg agaacatcga gacatgcgtc agggaatttt tcaatatcga tccgggacct
      481 ttacccaata ccggggtgag gctcaaaacg ctgccgttgg gtagagaaaa tacgagtatg
      541 atgatgagcg tacccaaaag atatgggccg taaagccaag ccacccggga aagagtttca
      601 atgccaagaa aaacaccata catcaataaa atcaagaaaa cggcggtaat cacgctgatc
      661 ggagtttggg gtaaaatggc caggatgaag ctctcggcga atttacgcaa aaacagggag
      721 gtggtgatga ggaagaacag aaagaataaa acaccggcgc cggtgccgat aaagtttccc
      781 gtgagcgttt gacagatctc aatgatgtct tgagaaggga aacgccggat gagggcatag
      841 agaaaggaaa aacccaccag cgccaagatc cccgtgagta tgacaatgat ccacccacta
      901 ccgtctccaa gggaggccat atccctggga aactgcaaaa acaccttgcc ggacatgata
      961 aaaaccaata atgtcagggc ctcgcgatgg cctattttgg tctcgtgctt catcagaacc
     1021 gctcgtcgca gagtgtttaa gactggtcgg agcttgagtc gcctccttcg ggatgggttt
     1081 cctctttttc aaccgcatcg ggacggtccc agggccgtgc cgtatctttt tggcggatcc
     1141 aatccagtgg acgcagaaag accggtcggt agggctggtt gaagttcaaa ggccggatca
     1201 cccggtcctt attttcgggc tggtacggag agatcgggga gaggaaggga accccgaaat
     1261 tggtcaggct ggccatgtgc agggttaaga gaaatatccc gaaggcgatg ccgtaaaatc
     1321 cgaggaatcc ggcaaacata acaaatatga accgcagtaa tctaatggta aacgaggcgt
     1381 tatagttggg aatcacgaat gaactcaggg cggtaatggc aatgacaatg atcagaatgg
     1441 ggctgacgat ggaagccgta actgcagcct ggcccagaat taacgccccg acgatcccga
     1501 tggtcggacc gatgacgctg gggatccgga tcccggcttc ccggattaat tcaaacgata
     1561 actccataaa aaggatttcg atgatcgacg ggaagggaac ggattcccgc gcggccgtca
     1621 ttgccaacag caggtcagtg gggatcattt cctgatgata attgatcacc gcgatataaa
     1681 aagccggcag gagcaaagcg gaaaagattg ccaccgcccg cagaaagcgc aaaaaatttc
     1741 cgaatggcca gcgcaggtaa taatcctccg ccgcatgcaa aaagatagtg aatgtcgtcg
     1801 gaataaccag ggcgtaggga ctgttggcca tgaccacacc cacaaaaccc tcgcgaagat
     1861 gggcacccat ccggtcgggg cgctcggtcg agagcatttg cgggatcagg gaatggggat
     1921 gatcctgcag gtattcctca agggtgccgg tctcggcgat ataatcggtt acccgggaga
     1981 ccgattggat gcggtactta acttcctcca caagctttgg gttggcgatg tccttgatgt
     2041 acataaaccc aaccagagta ttgctgcgtt tgcccacccg gaaaatctcc gtgattaatt
     2101 tgggatcttt aatatacctt ctgacactgg cggtgttgct acggaaggtt tcggtgaatc
     2161 cttcctgagg tccgcgcacc acatgctcgg tagagggctt ttcgataccg cggtgttccc
     2221 aacccttcgt ttcgatgaag agtgcctgag ccgagccttc aatcagcaag gcactggatc
     2281 cgtccaggac gttgtcgatg atttcctgaa acttccggcc tttgacgact tgattttcgg
     2341 gaagtaaccg ttcgtaaatc aaattgatcg gctccccgtc ggttccggcc ttttcctcta
     2401 ggatcatcag gggctgcagg atcgcgaagt tttgggtatt acgatcggta agaccatcta
     2461 tatatataag gatagcgttc aggtggccgg tgaagatttt aaactcacgg atgacaacat
     2521 ccttgtttag agggatctca aagatctcct ccaagcgggc ccggtttttt gccaggcttt
     2581 tggataaact ttcagcacct tcgggttctt t
//
LOCUS       DASVMB010000129         7485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_239132,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000129 DASVMB010000000
VERSION     DASVMB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239132"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VF531_04115"
     CDS             complement(<1..535)
                     /locus_tag="VF531_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713184.1"
                     /translation="MRSRNAAVTLADYKILAVDDDIAIIKAVKTLLKRNGYQCDGVTD
                     PLLALEKVRTGNYDLLILDYRMEPLRGDELVTEIRKFNQEIYILFFTGHQELAPPLKT
                     IKTLDIQGYCGKSDGFDRLLLAIESAFKSIARLRKVQNFRDGLNRILEAVPRICQTQS
                     IEATLEEILRGILPLVNS"
     gene            1044..1736
                     /locus_tag="VF531_04120"
     CDS             1044..1736
                     /locus_tag="VF531_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7713185.1"
                     /translation="MNYFKKVFACILTVTGLLVISIAPAFAYGNTYTYSIQRYSYVNK
                     GSYGFYIPGYYNGYRRFPTSPKQPTPTKPVPTLPTNPTTPKPPVTPPSNPTTPAPPTS
                     GLTTEESKMVNLVNQERTNQGIKPLSVNMSLVTLARKKSQDMVANNYFDHTSPTYGSP
                     FDMMKNAGISYKTAGENLAGASTTEIAHQNLMNSPGHRANILNTAFNQIGIGIASGSQ
                     YGNIFTQMFIGI"
     gene            complement(1852..2679)
                     /gene="nuoB"
                     /locus_tag="VF531_04125"
     CDS             complement(1852..2679)
                     /gene="nuoB"
                     /locus_tag="VF531_04125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7713186.1"
                     /translation="MFQMLRKIVQYPRLTVDYPRRPLKPADIIGKPEVDGSRCNGCGE
                     CIKRCPSAAIATGVSPQDIGFNLDQCIFCALCEDVCPTRAIRITSDFELAQKHRQDLR
                     KEALREVSPSTDARPSGVDAAASIDEDSYELMGRRIEERLRKKLGRSLQIREVDAGSC
                     NGCDYEINATCNPLNDLERFGIHFVASPRHADMLLVTGTACRNMELALVKTYNATPDP
                     KLVVAVGACACSGGIFQESPMVRKGIDSLVPVDVYIPGCPPRPQAIIYGILKAVERI"
     gene            complement(2710..4293)
                     /locus_tag="VF531_04130"
     CDS             complement(2710..4293)
                     /locus_tag="VF531_04130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7713187.1"
                     /translation="MGGTDVLEVLNHQFPGVIRNYQVCYENEFRCEVDPGEIGRIARF
                     LSDHLKMPLVSLFASDERVIRDGRFAIYYLFTLRPENQLWQFKTMISEADGIFPSITT
                     EIPAANLYERKIRDLYGLIPEGHPDPRGLVFHHNWPRDLYPSRRDYKGIKPLRGKAES
                     QFTRVDGSGVYEIPVGPVHAGIIEPGHFRFSVAGEPIIHLEAQLHYVHKGIEKLAEGQ
                     SIEKCFYIAERISGDETFANSMAYCQAIERIAGVQIPVRAEYSRVVFAELERLVNHFG
                     DLAGICTDVAYGFAASQFNMLRIWAYQTTEEMGRSRFLRSVNRIGGLRRDFLAGKEER
                     LLQQLARMKAELTETEELIKANSLFVDRVEHTGILATEIARDLNAVGPAGRASGLKID
                     VRRDHPYAAYGKLRLEVPEHANGDVNCRMNVKISECYGAMDLITQALSQMPSGEFFIP
                     IAGIEPYKSALGYTESPRGENIHWIMSGPGDTIFRYKIRTPSFCNWPALCYAVKGNIV
                     PDFPLINKSFNLSYAGNDL"
     gene            complement(4294..5772)
                     /locus_tag="VF531_04135"
     CDS             complement(4294..5772)
                     /locus_tag="VF531_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX7713188.1"
                     /translation="MEIMILIIPLIGMLLFWATTNPKALHCINGAGTLMLFVITLLIT
                     SKVSLHGPISYPYLNHFFYLDALSVIVLDTVAIVSLMVSIFTIGYMNQELRHGEITPR
                     RLQLFYSLMYAFVFTMVLVVSTQNLGLMWIAIEATTLASAFLVGFYNHQESIEAAWKY
                     VIICSVGIALALLGIVFLYISAVNIFEGSKSSLNWLFLLRNASSLHSSILKISFIFVL
                     VGFGTKIGLVPMHTWLPDAHSQAPAPISALLSGVLLNSALYGLIRILAIVNKNLGSHL
                     YTGRLLLGAGLVSIATAAVFIMTQKDYKRMLAYSSIEHMGVITFGLGIFTPASLFAAL
                     FHMVNHAFTKSLLFLSSGNVYLKYKTREIAGVRGLLKTLPVSGVCFLLGLFAIAGLPP
                     FSVFSSELNLIISAFAAGQYGPAALFICLVAAVFAGLVLVAMKMFFGNDLSPQIEPGE
                     QNWLGLPVLIILLLVVAVTGVFMPERFKQLLEAASGIIRGVA"
     gene            complement(5777..6433)
                     /locus_tag="VF531_04140"
     CDS             complement(5777..6433)
                     /locus_tag="VF531_04140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEX7713189.1"
                     /translation="MDNFLQFLSIAILISGFILVADKRVNSYIKMFRLQSVLLAVMAG
                     FIGINSIILHGDWEIMIVALLTLGIKAIYIPHLLKTTVRRVEYKVEKDFFLNIPISIL
                     ICCGLVILSYFTMTHIQDIAENDLKIYLANAMAVVLIGLFFMISRKKAIGQIIGFLVI
                     ENGLFTAAILSTEGMPMIVELGIFFDVLTAVLIMGILVFRINENFETINLDKLKNLRG
                     "
     gene            complement(6436..7386)
                     /locus_tag="VF531_04145"
     CDS             complement(6436..7386)
                     /locus_tag="VF531_04145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX7713190.1"
                     /translation="MSYIYNTILQLAVIITLAPLVNGIIKKVKALSQKRKGPPLLQTY
                     YDLLKLFKKDTVVSATASWIFKATPYITIGATLCAAVLIPVSLKVQPIRFMGDVILAV
                     YLLALARFFMVLAGMDAGSTFGGMGSSREMMISSLTEPSLLVTVFTIGLLSGSTAVFD
                     MMQSTARLGWDLFQPVYLLTFLALFIILITETARIPVDDPATHLELTMVHEAMLLEYS
                     GRYLALMELGASIKQLLFITLLVNLFLPTDGLIAITNPVMTVLASLGFYFIKVIVIAV
                     TMGIVEIHSVKLRLFSVPNLAALSFILALLGFFQYWIIGR"
BASE COUNT         2164 a         1959 c         1629 g         1733 t
ORIGIN      
        1 tactgtttac caggggtaaa attccccgaa ggatctcttc cagggtcgct tcgatagact
       61 gggtttggca aatcctgggg accgcctcta ggatccggtt caacccgtcc cggaaattct
      121 ggaccttgcg caacctagcg atagatttaa acgcggattc gatcgccagc agcaatctgt
      181 cgaaaccatc actttttcca caatatccct gaatatcaag ggtcttaatc gttttgagcg
      241 gtggggctag ctcctgatga ccggtaaaga acaaaatata gatttcctgg ttaaacttcc
      301 ggatttcggt cacaagttca tctccgcgga gcggctccat ccggtaatcc aagatcaaca
      361 gatcatagtt cccggtacga accttttcca gcgcaagcag gggatcggtt accccatcgc
      421 actggtaacc gttccgtttt agtaacgttt taaccgcctt gatgattgcg atatcatcat
      481 caacggctaa gattttgtaa tccgccagag ttactgccgc gttcctactt ctcaccggta
      541 caccccgctt ctaacacatc cattaaccta ctgctgataa agcaagggat attgaaactg
      601 cggaccgaaa acagcaacgc taatacgata taacggtatc agccgatata tcggattgca
      661 aaatatgtta cctgaatccg aacttaacca ccattacaat ctaaacccac ctctataaaa
      721 tgatatcaat atattcaagg ctccatcaac tttccctgtc aaaattggaa aattttttcg
      781 taaaacttag caaatttggg gtcatatcca ttctccagcg gcggtgatca ccagctcgca
      841 gtgatccacg cctttggtgc tgctccgcca ttctttgtat ccccgggccg cttcccttga
      901 caactaaaat ttccttgatt ggccatagtt tcctaaaggg atcaagagct taccaccgaa
      961 atcatgttat accaaaagag ttgggggctt gaaaatctgg gactaattgt aaaacaaaaa
     1021 cagatctgaa aggagcgacc ttcatgaatt atttcaaaaa agtgtttgct tgcatcctaa
     1081 ctgttaccgg attactggtg atcagtatcg ccccggcctt cgcctacggc aatacttaca
     1141 cctactccat ccagcggtat tcatacgtaa ataaaggcag ttacggtttt tatattcccg
     1201 gctattacaa tggatatcgt aggtttccaa cttcaccgaa acaaccgact ccaacaaagc
     1261 cggtgccgac tctaccaacg aatcctacta ccccgaagcc gccggtaact ccgccgtcga
     1321 acccaacgac tccggcacct ccaaccagcg ggctcacgac tgaggagtcc aagatggtaa
     1381 acctggttaa ccaggagcgg accaatcagg gtatcaaacc cctgagtgtg aatatgtccc
     1441 tggtaacatt ggcccgtaag aaaagccagg atatggtggc caataattat ttcgaccata
     1501 cctcgcccac ctatggatca ccgttcgata tgatgaaaaa tgccgggatt agctacaaga
     1561 ccgccggaga aaatctcgcc ggtgcaagca ccactgagat tgcacatcaa aacttgatga
     1621 atagccccgg tcacagagcc aatatcctga atacagcctt taaccagatt ggtatcggta
     1681 tcgcctccgg aagccagtac ggcaatatct tcacccagat gttcatcggg atctaacgaa
     1741 tcccttacaa cctgactctt ggtctagtat caatacggga ggccacagcg cctcccgtat
     1801 tatttatctc aaaaaatccc gaagcccttc tggaatgcga agccaaccgg actaaattct
     1861 ttccaccgct tttaatatcc cgtaaatgat cgcctgaggc cggggcggac accctggaat
     1921 ataaacatcg accggtacca ggctgtcgat acccttacgg accatcgggc tctcctgaaa
     1981 aatcccgccg ctgcaggcgc aggctccaac cgccacgacc aacttgggat cgggagtggc
     2041 attgtaggtc ttcaccagcg ccaattccat gttccggcag gcggtaccgg taaccaaaag
     2101 catatcggca tggcggggtg aagctacaaa gtgaatgcca aaccgttcca aatcatttaa
     2161 cgggttacac gttgcgttaa tctcgtaatc acagccgttg catgaaccgg cgtctacctc
     2221 ccggatctgt aaactccggc ccagcttctt gcggagccgt tcctcaatcc tgcgccccat
     2281 caactcataa gagtcctcat cgatagatgc cgcggcatcg acacctgatg gccgggcatc
     2341 ggttgaaggc gaaacctctc ttaaagcctc tttgcgtaaa tcctgacgat gtttttgggc
     2401 cagttcaaaa tcggatgtaa tccggatggc acgggtgggg catacatctt cacacaaggc
     2461 gcaaaagatg cattgatcaa ggttgaatcc gatatcttga ggagatactc ccgtagcaat
     2521 cgccgcactc ggacaacgtt tgatgcactc accgcaacca ttgcatcgtg atccgtcgac
     2581 ctcaggttta ccaatgatat ccgcgggttt taaaggccta cggggataat ccacagtcaa
     2641 gcggggatat tgaacgatct ttcgtaacat ctggaacatg gctatcatcc attctacaaa
     2701 aataaattat tatagatcat ttcctgcata ggacaggtta aagctcttat tgatcaacgg
     2761 gaaatccggt acgatattgc ccttgaccgc ataacaaagc gcaggccagt tacagaagga
     2821 gggagtcctg atcttatagc ggaaaatggt atctcccggg ccggacataa tccagtggat
     2881 attctctcct cttggcgact cggtataacc aagagcggat ttatatggct ctatcccggc
     2941 aatagggatg aaaaactctc cggacggcat ctgactgagc gcctgggtga ttaaatccat
     3001 cgcgccatag cattcggaga tctttacgtt catccggcaa tttacatccc cgttggcgtg
     3061 ttccggtact tcaaggcgaa gtttgccata tgccgcatag ggatgatccc gacggacatc
     3121 gattttcaac cccgaagccc tgccggcagg gccaaccgca ttcaaatccc gcgcgatctc
     3181 ggtcgctaaa attccggtat gttcaaccct atcgacgaac agactattgg ccttaatcag
     3241 ctcttcggtc tccgtcaact cggccttcat ccgcgccaac tgctgaagca agcgttcttc
     3301 cttgcctgct agaaaatccc ggcgtaaacc gccgatccga ttcacgctac gtaggaaacg
     3361 actccggccc atctcttcgg tggtctgata agcccagatt cgcaacatat tgaactgaga
     3421 cgcagcgaag ccataggcta catcggtaca gattcccgcc aaatccccga aatggtttac
     3481 caaacgttcc aattccgcaa aaactacccg ggagtactcc gcccgaacgg ggatttggac
     3541 tccggcgata cgctcgatcg cctggcaata ggccattgaa ttggcaaagg tttcatcacc
     3601 ggagatacgt tccgcgatat aaaagcactt ttctatgctc tgcccctcgg ctaacttttc
     3661 aattccttta tggacataat gaagctgggc ttccaagtga ataatcggtt caccggcgac
     3721 actaaaccgg aaatgtcccg gttcgataat tccggcgtgt accggcccta ccggaatctc
     3781 atagacaccg ctgccatcaa cccgtgtaaa ctgcgactcg gcctttccgc gcagcggttt
     3841 aattccttta taatcccggc gtgaaggata caaatcccga ggccaattgt gatgaaacac
     3901 cagaccccgc ggatcggggt gaccttccgg gatcaaaccg taaagatcgc gaatcttcct
     3961 ttcataaagg ttggctgcag gaatctctgt tgtaattgag ggaaatatcc catccgcctc
     4021 ggaaatcatg gttttaaact gccataattg attctctggg cgaagggtaa atagataata
     4081 aatggcaaac cgaccgtcgc ggatgacccg ttcatcactg gcgaagaggg ataccaaagg
     4141 cattttaaga tggtcactca aaaaacgagc aatcctgcca atctcccccg gatcaacctc
     4201 gcaacgaaat tcgttttcgt aacagacttg ataattccgg atcacacccg gaaattggtg
     4261 atttaagacc tcaagtacat cggtgccacc catctatgca acccccctta taatcccgga
     4321 agccgcttcc aaaagctgct taaaccgctc cggcataaat actcccgtaa ccgcaacgac
     4381 cagcaacaag atgattaaca ccggcaaacc cagccagttc tgctcccccg gttcaatctg
     4441 cggggataga tcgttgccaa agaacatttt catggctacc agaaccaggc cggcgaagac
     4501 cgccgcaacc agacagataa atagtgccgc cggtccgtac tgccctgcag caaatgccga
     4561 aataatcagg tttaactcgc tgctgaaaac actaaacgga ggtaggccgg caatggcaaa
     4621 caagccaagt aaaaaacaca caccgctaac gggtagggtt tttaataaac cccttacccc
     4681 ggcgatctct ctggttttat atttcaaata aacgttaccg gaggataaaa agagcaatga
     4741 cttggtaaag gcgtgattca ccatatggaa cagggcggcg aacaacgaag ccggtgtaaa
     4801 aatccccagc ccgaaggtga tgacgcccat atgttcaata ctggaataag ccaacatccg
     4861 tttgtaatcc ttttgagtca tgataaacac agcggccgtt gcgatggaaa ccaagcccgc
     4921 tcccaacagt aaccgcccgg tatacaggtg actccccaaa tttttgttga cgatcgccag
     4981 tatcctgatg agtccatata gagcgctatt tagcaagacc ccggacaaca aggcgctaat
     5041 cggcgccggc gcttgactat gggcgtcggg cagccatgta tgcatcggca ccaaaccgat
     5101 cttggttcca aagccgacca aaacaaagat aaacgagatc tttaaaatac tgctatgaag
     5161 ggaggaagcg ttccgcagta agaataacca attcaaactg gactttgagc cttcaaaaat
     5221 attcaccgcc gagatataaa gaaaaacgat ccccagtagt gccagggcaa tacctaccga
     5281 acagataatc acatacttcc aggcggcttc gatcgactcc tggtggttgt aaaagcccac
     5341 caaaaatgcg gaggcaagcg tcgtggcttc aattgcaatc cacatcaaac cgaggttttg
     5401 ggtactcacc accagcacca tggtaaagac gaaggcatac atcaaactgt agaataactg
     5461 taaccttcgg ggagtgatct ctccgtgacg cagctcctga ttcatgtaac cgatggtaaa
     5521 tatgctgacc atcaaactaa cgatggccac agtatccagc acaatgacac ttaaagcgtc
     5581 aagatagaaa aaatgattca ggtaaggata actgatggga ccgtggagac tcacctttga
     5641 agtgatcaat agggtgatga cgaagagcat caaggtcccg gcgccgttga tgcaatgaag
     5701 cgccttcggg ttcgtagttg cccaaaataa caacatccct atcaagggaa tgattaaaat
     5761 catgatctcc atgcttttat ccccgcaaat tctttaattt gtccagattg atcgtttcaa
     5821 aattctcgtt gatccggaaa accaagatac ccatgattaa caccgcggtc aatacgtcga
     5881 aaaagatacc caactctaca atcatcggca tcccttccgt tgaaagaatg gctgccgtaa
     5941 ataggccgtt ttcaatcact aaaaagccga taatctgtcc gatggccttc ttacggctga
     6001 tcatgaagaa caggccgatt aagacgactg ccatcgcatt ggccaaatat atcttgagat
     6061 cgttctcggc aatatcctga atatgggtca tggtaaagta ggagagtatc acgagcccgc
     6121 aacatatcaa aattgagatc ggaatattca ggaaaaaatc cttttctact ttgtactcta
     6181 cccggcgaac cgtggttttc agtaaatgag gaatataaat tgccttaata cccagcgtca
     6241 gcagggcaac aatcatgatc tcccaatcac catggagaat tatgctgttg atgccgatga
     6301 atccggccat aacagccaat agcaccgact gcaagcgaaa cattttaatg taggagttca
     6361 ctcttttgtc cgccactaag ataaacccgg aaatcaaaat ggcgatcgat aaaaactgca
     6421 agaaattatc cacagttacc tcccaataat ccaatattga aagaatccca gcaaagccaa
     6481 gataaatgag agcgccgcca gattggggac agaaaacaac cgcagtttga cactgtgaat
     6541 ctccacgatt cccatcgtaa ccgcaatgac gatcactttg ataaagtaaa aaccgagtga
     6601 tgccagtacc gtcatgaccg ggttggtaat ggcaatcaag ccatccgtgg gcaaaaacag
     6661 attgaccaga agagtaatga acaataattg tttaatggaa gcccccagct ccatcagggc
     6721 caagtagcga ccggaatatt ccaataacat ggcttcgtgg accatggtca gttccagatg
     6781 agtggcggga tcatccacgg gaatccgcgc ggtttcagta attaaaatga taaatagtgc
     6841 caaaaaagtc aacaaataaa ccggctgaaa aagatcccaa cctaaccggg ccgtgctttg
     6901 catcatgtca aaaactgccg tagaaccgga caatagcccg atggtaaata ccgtgaccaa
     6961 cagggaaggc tctgtcaagg aactgatcat catctcccgg ctgctcccca tcccgccaaa
     7021 ggtacttccc gcatccatgc ccgccagtac catgaagaaa cgggccaggg ccaataaata
     7081 taccgccagg atcacatcac ccatgaaacg gattggttgg acttttagac ttaccgggat
     7141 taataccgcg gcacataggg ttgccccgat tgtaatatac ggcgttgctt taaagatcca
     7201 ggatgcagtg gcggagacta ccgtatcctt tttgaataat tttaaaagat cataataggt
     7261 ttgtaaaagc ggtgggcctt ttcgcttttg cgagagggcc ttgacttttt tgataatccc
     7321 gttaaccagt ggggctaagg taattatcac agctagttgc agaattgtgt tatatatgta
     7381 actcattcag acctccttaa ccgcagaaac ggaaatatac caataacaac aagacggtaa
     7441 caaatatata tatcaaatac cggtgaatac ttccggtttg aatga
//
LOCUS       DASVMB010000130         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_239140,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000130 DASVMB010000000
VERSION     DASVMB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239140"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..903)
                     /locus_tag="VF531_04150"
     CDS             complement(172..903)
                     /locus_tag="VF531_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713191.1"
                     /translation="MEIAEALVKVGFTRQESLLYLTLCREGPMTGYEASKGTGISRSN
                     AYLALAGLVEKGAAYRTEGEAVKFIPVPAGELTRRLRDELGRVLDFIAANAPPAELPR
                     EPFITIEGRNAIIRELQDLIGMAKERIYLAADSAELNGIMAELTAAVCRGLKVVVITA
                     SPWNLTGATVYYHAKTPGQLRLIVDSACVLTGELSGAGHSACIFSRNNNLVQLIKDSL
                     TNELELIGRKDRPMETMNPEGESHE"
     gene            1081..1803
                     /locus_tag="VF531_04155"
     CDS             1081..1803
                     /locus_tag="VF531_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713192.1"
                     /translation="MEQALVVEDLRKSYGKHEALKGISFEVRPGEIFALIGPNGAGKT
                     TTLRMIATILTPSSGSSKVYGLDTVDDGEKVRQIISYLPEEAGAYKNMTGNAYLRFMA
                     ELFTPDRHTRDEYVAAAAAITGLGDRLNDRIGTYSKGMTRKLLLARAVMTHPKLAILD
                     EPTSGLDVVNALEIRQAIKDLAKEGMAVLVSSHNMLEIEYLSDRVAIIDRGVIHRTGT
                     PAELKAAYQAANLEEVFVEVVR"
     gene            1800..3113
                     /locus_tag="VF531_04160"
     CDS             1800..3113
                     /locus_tag="VF531_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713193.1"
                     /translation="MNRFATLLRKELRELMTLQMILPFVFVVMIFFFIGNIAGNETRK
                     AAAPQWIAVLDQDRSPLSAELTGLLATQNFRIRSFHDQTLTKTLRQAKDEKIATVIVI
                     PRGLDREIAAARQVPIPVYSIISNFSLLGSLGSQAAKGAVSTINDYLSDRLLAQKAAR
                     APASNPASLKNPVTAKNYVLIGSRSAAITPDLVSGFITSQTAFLPIVMFIIIVIASQM
                     IAVSIASEKENKTLETLLSTPISRVSLVTAKMVAAGIVSLLIAVFYMFGFQSYMNGVT
                     GGFNSTAAHNPQLAAAIAQLGLTLDLPAYLLLGASLFLSILIALAISLILGAFAENVK
                     SAQGLIAPITFLIMIPYFLTMFIDLNTAAPLLRFIVYAIPFSHPFLAGPFLFLHNYPA
                     VVYGLVYQAVFFIILVIVAARIFASDRVLTMKLNFKKGRFQIRQD"
     gene            complement(3281..4096)
                     /locus_tag="VF531_04165"
     CDS             complement(3281..4096)
                     /locus_tag="VF531_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713194.1"
                     /translation="MKTKTGVDRSARAPSRIGLAKERSLHAAIKEWYAEPGDEFEVGV
                     DGYVIDLVRPAGPDGRKLLIEIQTRNFTAIAPKLQALVARYQVRLVYPIPRRKTIIRI
                     TADGEVCGSRRSPKSGTALDLFRELVRIPKLINEPNFSIEVLLIEEEEIRRADGRGSW
                     RRKGVSIADRRLMAVEERLLFRESDDFRCFLPPDMPLPFSNKEYACFCKCSIYLARKL
                     TYCLKKMGLLYQVGKRGNQLLYAVRDFEGKVSSQAAGYTAPNVESLAVADREE"
     gene            4363..>4993
                     /locus_tag="VF531_04170"
     CDS             4363..>4993
                     /locus_tag="VF531_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7713195.1"
                     /translation="MPYRYRRGFLFLLAFTGILLLPVPLAQVSGADSRPARGGRQIII
                     AIVPKSLDNPVFVDAKEAAELTAREHRVLLEWVGPFKVNPDEQVRIIEGLIWRKVDAI
                     AISCSDPKKIRGVIDRAAAAGIKVATMDADCPESQRLFYCGTDNYRAGLACGKAMVRI
                     IAEKGLAKKSLRTAILTGGIEADNLNERIRGFKDAVSGKILLNYVALLHC"
BASE COUNT         1165 a         1390 c         1240 g         1198 t
ORIGIN      
        1 gagtaatgga ctttgaaacc cggcaaagag agcaattgtc tcatctcacg gcaacgggcc
       61 cgaagcaccg cctcattata aacatagagc gggcttccga aatcggcgat taacttgcgg
      121 ggattggtgg caccataaaa acccatggta ccggtgaaat cgaaagtttg ttcattcatg
      181 ggattcaccc tccggattca tagtttccat cggtcgatcc ttcctaccga tcagctccag
      241 ctcattggtg agggagtctt taatcaactg gaccagattg ttgtttcgtg agaaaatgca
      301 ggcggaatgc cctgcgcccg aaagttctcc tgtgagaacg caggctgagt cgacaatcag
      361 ccgtaactgc ccgggggttt tagcatgata atatacggtg gcacctgtta gattccaggg
      421 cgaggcggtg atcaccacta ccttgagccc gcggcatacg gcagccgtta actccgccat
      481 gataccattc aactccgccg aatcagccgc cagatagatc cgttcctttg ccatgccgat
      541 caggtcctgc aactcccgga taatggcgtt cctgccctcg atggtaataa acggttctcg
      601 aggcagttct gcaggggggg cattggccgc gataaaatcc aacacccgtc ctaattcatc
      661 cctgagccta cgggtgagtt cccctgcggg gaccggaata aacttcactg cctcgccctc
      721 agtccggtag gccgcgcctt tttcaactaa tcccgccaaa gccagatagg cattggaacg
      781 ggaaataccc gtgcctttag acgcttcgta tccggtcata ggtccctcac gacataatgt
      841 caggtatagc agggactctt gccgggtaaa accaaccttt accaatgcct cagcaatttc
      901 catcgccggt tcctcctagt ggtactattt agtatcacta taatccctgg attaattttt
      961 gtcaacaatc aacattctcg gcaggaattc ccctatggcg gtggtatgat ccaattcaaa
     1021 ggtttcatac taagtaaaac ttcccgacta acaagattcc aaatatacgg aggtttaaga
     1081 atggaacagg ctttagtggt cgaggatttg agaaagagtt atggaaaaca cgaagcgctc
     1141 aaggggataa gtttcgaagt ccggcccgga gagatctttg ccctgatcgg acccaacggg
     1201 gcaggaaaaa ccacgactct acgaatgatc gccacgatcc ttaccccaag tagcggcagt
     1261 tccaaagttt acgggttgga tacggtcgac gacggagaga aggttcgcca gatcatcagt
     1321 tatcttccgg aagaagccgg cgcctataaa aacatgaccg gtaacgcata ccttcggttt
     1381 atggcggagc tcttcacccc ggatcgacat acccgtgatg agtatgtcgc tgctgcagcg
     1441 gcgatcaccg ggttgggtga ccgcctgaac gaccggatcg gcacctacag taaaggtatg
     1501 acccgtaagc tgcttttggc cagagcggtg atgacccatc ccaaacttgc cattcttgat
     1561 gaacccacca gcgggttaga tgtggtcaat gccctcgaga tccggcaggc catcaaggat
     1621 ctagccaagg aaggcatggc agtattggta tcgagccata atatgctgga gatcgagtat
     1681 ctctccgacc gggtcgctat tatcgatcgg ggtgtgattc atcgcaccgg taccccggct
     1741 gagttgaagg ccgcatacca ggcggcaaat cttgaagaag tcttcgtgga ggtggtccga
     1801 tgaaccgctt tgctaccttg ctccgaaaag aactgcggga actgatgacc ctgcagatga
     1861 ttcttccctt cgtctttgtc gtcatgatct tttttttcat tggtaatata gccggaaacg
     1921 agacccggaa ggctgcggcc ccccaatgga ttgcggtttt ggatcaggat cgatcgccgc
     1981 tttccgcgga actcaccgga ttactggcaa cccaaaactt ccggatccgg agtttccatg
     2041 accaaacgct gacaaaaacg ctccggcagg ccaaggatga aaagatcgcc acggtgatcg
     2101 tgatcccccg gggattggac cgggaaatcg cggcagcccg acaggtgcca atcccggttt
     2161 atagtatcat cagtaacttc tctctcttgg gttcactcgg ctcccaagcg gccaaagggg
     2221 cggtttcaac cattaacgat tacctcagcg accggctttt agcccaaaaa gcggcgcgcg
     2281 cgcctgcgag taatccggct tccctaaaaa acccggtcac cgccaaaaat tatgtgctta
     2341 tcggttcgcg gagtgccgcc atcacgccgg atctagtctc cggcttcatc acgtcgcaaa
     2401 ccgcctttct gccgatcgtc atgtttatca ttattgtcat tgcgtcgcaa atgatcgcgg
     2461 tgtccatcgc atcggagaag gaaaataaaa ccttggaaac cttgctttcc acgccaatca
     2521 gccgtgtttc cctggttacc gcgaagatgg tggccgccgg aatcgtctcc ttgctcatcg
     2581 cggtctttta tatgtttggt ttccaatcct atatgaacgg cgtcaccggc ggtttcaact
     2641 cgacagcggc ccataatccg caattggcgg cagccatcgc ccaattaggc ttgacgctcg
     2701 atcttccggc ctatctgttg ctgggtgctt cgctcttttt aagcattctc atcgccctgg
     2761 cgatctcgct tatcttaggg gccttcgccg agaatgtaaa aagcgcccag gggttgattg
     2821 ctccgattac gttcttgatc atgatcccct attttttgac gatgtttatc gatctcaaca
     2881 ctgccgcccc cttactccgg ttcatcgtct acgccattcc cttttcccat ccgtttctgg
     2941 ccggaccgtt cctgttcctc cacaactatc cggccgttgt ctacggcctg gtctaccaag
     3001 ccgtcttctt catcatcctg gtgatcgttg cagcccggat cttcgcctcg gatcgggtgc
     3061 ttaccatgaa actgaacttt aagaaaggta ggtttcagat ccgacaggac taataacaaa
     3121 acggaccgtt atcccgggaa tacgagccta taaacccgta tttcccggac tcggtccgga
     3181 taatcactaa gctacgcttc attgacatat cgacatcctt aattcgcaac aaccgctttt
     3241 acaatgtaag tatcagaccg atatgttcct ccgccgcaag ttactcttcc cgatcagcca
     3301 ccgccaagct ttcaacattt ggagcagtgt atcccgccgc ttgcgatgaa accttccctt
     3361 cgaaatcccg aacggcgtag agcaactggt tacctctttt tccgacttgg taaagtaacc
     3421 ccatcttttt cagacagtat gtcaatttcc gtgccagata aatactgcat ttgcaaaaac
     3481 acgcgtattc cttgttagaa aaaggcaatg gcatgtccgg aggcaggaag caacggaaat
     3541 cgtcactttc cctaaataac aacctttcct ccacggccat caaacggcga tcggcgatac
     3601 tgactccctt ccggcgccag ctgccccggc catcggcccg gcggatctcc tcttcctcga
     3661 tgagcagcac ctcgatggag aaattgggtt cgttgatgag cttcggaatc cggacgagct
     3721 ctctgaagag gtccagcgca gtaccggact ttggggaacg gcggctgccg catacctcgc
     3781 catcggccgt aatccggatg atggttttcc ggcgggggat ggggtaaacc agccgtacct
     3841 ggtacctggc gaccagggct tgaagtttgg gcgcgatcgc cgtaaaattg cgtgtctgga
     3901 tctcgatcag cagcttccgg ccatccggcc ccgccggccg taccagatca atgacgtaac
     3961 cgtccacccc gacctcgaac tcatccccgg gctcagcgta ccattccttg atagcggcat
     4021 gaagcgaacg ttccttggcg agtccgatcc gggaaggggc gcgtgccgaa cggtccacac
     4081 cggtttttgt tttcatcaat gcgccacttc acttccgaga aattctaggg gtatctttta
     4141 tttatttcga ttcggacggg gttttccctt caaaatgaga agacctcttg ccaaatgaag
     4201 tatcatttca aaaaatagaa taatcaatga taaaaatgat atataatgta tacggaatgt
     4261 ttcgtaaaga ttgcactaca aattgtcgca tcgacggatg caagcggaaa cacggaacac
     4321 ctcccccgtg ggttacaacc tttacggtca ggagtgattt ttatgcccta tcgctatcgc
     4381 cgtgggtttc tattcctctt ggcgtttacc ggcattcttt tacttcccgt cccgctcgct
     4441 caagtatccg gagcggattc ccgtcccgcc cggggtggcc ggcagattat catcgccatc
     4501 gttcccaagt cactcgataa tccggttttt gtcgatgcca aagaagcagc agaattgaca
     4561 gcccgggaac atagagtcct tctggaatgg gtggggccgt ttaaggtcaa ccccgatgaa
     4621 caggtacgta tcatcgaggg cctcatctgg cgtaaagtgg acgctatcgc tatcagttgc
     4681 agcgatccga aaaagatccg gggggtaatc gaccgggccg cagccgccgg aatcaaggtg
     4741 gccacaatgg atgccgactg tcccgaaagt cagcggctct tttattgcgg caccgataat
     4801 taccgggccg gtctcgcctg cggcaaggcg atggttcgga tcatcgccga gaaagggttg
     4861 gccaaaaaga gcttgcgcac tgccatactt accggaggca ttgaggccga taacctcaac
     4921 gagcgcatcc ggggctttaa ggatgcggtt tccggcaaaa tcctcctaaa ctatgtggcc
     4981 ttactccact gcg
//
LOCUS       DASVMB010000131         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_17270,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000131 DASVMB010000000
VERSION     DASVMB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17270"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2424)
                     /locus_tag="VF531_04175"
     CDS             complement(<1..2424)
                     /locus_tag="VF531_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HEX7713196.1"
                     /translation="MDKDRVNDEATASPETPDVPKKKKTVKPKPVEVIEKASTKKKSK
                     TKSKGTTTKKRTAKTKKTLLTNQPEVEEGLQPDPIINDLMIHVQQMPPEASVFSGEVP
                     QKVEVGMLSNQTANPAVPYRAKAVLKTGSLSMGQVEAGSKSAEVLTSQGRQKPVLTGT
                     KLKVSALLDSPSASDSESLNRRDGSSNSASESMDGKSMSSAAFDSMPVPVNQFIQPNQ
                     PVQPFQPMQSETVVLSGSGQVCASQNAILPLTAIKVRNIVAKVVKLTAEIIPNKVIVQ
                     GVVHEQIFFVATDGIVHHMADDVNFSTFIDIPGAEPGMNAEASAEIEDVISELALDGL
                     SILKKIIIEVFIKVTETVQVNLEQGNGPIVLLNQVIGENSAQTLVESDFTLGIPALKI
                     DEIVGTVRDLEVETIKDKVIIQGTLHKQIFFIDKANFGRHEAEDLHFSLFVDIPGATP
                     GMDVQVHPRIEAIFFNLISPVCLRQKAVLEFFVKVTESVQQPVTVGSGSLFKVEEFIG
                     ENTVQELSESLITLATPAIKIREIVARVRDETIHVIQDKVIVQGIIHKQIFFISTDNI
                     EHHQAEDVPFSLFLDIPGASPGDNVHLRPVIEAVFFELVSCNQLRQKIILAVNAVVTR
                     FAQLNLVNGLGGPLFKIEQVIGENTRQVLIVERVRIAPTPVSPVEVAQVTVVFPSEVV
                     VAEQQTIVQNQVLLPIQAIKVKEIQPSIANLVFNVVPDGVVVEGFVCKTVFFVATDNI
                     VRTVHEKVPFSVLVSTPGITASQISNVFVEIENVSFNLNATCTAVNQIIVLKAIVEGS
                     SVTPSSVTVI"
     gene            complement(2713..>3083)
                     /locus_tag="VF531_04180"
     CDS             complement(2713..>3083)
                     /locus_tag="VF531_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7713197.1"
                     /translation="EAEMVSLINQERAKAGLPDLQVDPTLEQLAETKSRDMVRFNYFG
                     HSSERFGTVYDQLEHAGVKYQIVAENLIGASSCIKALQSENQSPAHRSNLLNPNFTRI
                     GVGVVRGGPYGLMITQILIR"
BASE COUNT          746 a          758 c          731 g          848 t
ORIGIN      
        1 aattaccgta acgcttgaag gtgtaaccga ggagccctcg acgatcgcct tgagaactat
       61 aatttgattg actgcggtgc aggtcgcgtt caaattgaag gagacatttt caatctctac
      121 gaaaacatta ctgatctgac tcgccgttat ccccggagta ctcacgagta ctgagaaggg
      181 caccttttcg tgaacggtac ggacgatatt gtcggttgct acgaagaaca cggttttaca
      241 gacgaatcct tccacaacca ctccatcagg taccacgttg aagaccagat tggcgatgga
      301 cggttgaatc tccttgactt ttatcgcctg aatcggtaac agaacctggt tttgcacgat
      361 ggtctgctgt tccgccacga cgacctcgga cgggaaaacc acggtaacct gagccacctc
      421 aacaggtgaa acaggcgtcg gagcaatccg gactcgttcg acaatcaata cctggcgggt
      481 gttctcgccg atgacctgtt ctattttgaa gagtggtccg ccaaggccgt tgaccagatt
      541 cagctgggca aaccgggtga ccactgcgtt aaccgcgagg ataattttct gccgcaattg
      601 attgcatgag accagttcga agaagaccgc ctcaatcacc gggcggagat ggacattatc
      661 gccggggctt gcgccgggga tatcgaggaa tagtgagaac ggaacatcct cggcctggtg
      721 atgctcgatg ttgtctgtgc tgatgaagaa gatctgctta tgaatgatgc cctgaacaat
      781 caccttatcc tgaatcacgt gaatggtctc atcccgaacc cgggctacaa tctccctgat
      841 cttaatcgcg ggagtggcaa gggtgatcaa ggactcactc aattcctgaa cggtattttc
      901 acctatgaac tcctctactt tgaagagaga tccgctgcca accgtgaccg gctgttgcac
      961 actttcggtc accttgacga agaattccaa aaccgccttt tggcgcaaac aaaccggact
     1021 gatcaaatta aagaagatcg cttcaatccg cggatgaacc tgtacatcca ttccgggggt
     1081 cgcacccggt atatccacaa acaagctgaa atggagatcc tctgcctcgt gtcggccgaa
     1141 gttggctttg tctataaaga atatctgctt atgaagcgta ccttggataa tgaccttatc
     1201 tttgatggtt tcaacttcta gatcccgtac ggttcctaca atttcatcga tcttcagtgc
     1261 cggaataccc agggtgaaat ccgactccac caaggtttgg gcggaattct ctccgatcac
     1321 ctgatttagc aagacaatcg gaccgttacc ttgttccaga ttgacctgta ccgtttctgt
     1381 gactttgatg aagacctcga tgatgatctt tttcaggata cttaagccgt cgagggccag
     1441 ttccgagatg acatcctcga tctccgcgct tgcctcggca ttcattccgg gttccgctcc
     1501 ggggatatcg atgaaagtgc tgaagttcac atcatcggcc atatgatgta caatgccatc
     1561 cgtagccaca aagaagatct gctcatgaac aacgccctgg acaattactt tattgggaat
     1621 gatctccgca gtcagcttaa cgaccttggc tacgatatta cgaactttaa ttgcagttaa
     1681 gggcaaaatc gcattctggc tagcacacac ttgcccacta ccgcttaaaa caacggtttc
     1741 cgattgcatg ggttggaacg gttgaacggg ttgattcggc tgaataaact ggtttactgg
     1801 aaccggcatg ctatcaaatg ctgcactact catagatttt ccatccattg actctgaagc
     1861 cgaattggat gaaccgtctc ggcgattcag actctctgaa tccgatgcgc ttggtgaatc
     1921 cagtaacgct gataccttga gcttcgttcc ggttaagaca ggtttttggc gaccctgaga
     1981 tgtaagaacc tccgcagatt tggaaccggc ctctacctga cccatggata gagaccccgt
     2041 ttttaaaacc gcttttgctc tatatggtac ggcaggattt gccgtctgat tcgataacat
     2101 gcctacttct actttctgag gaacttcacc cgaaaaaacc gaggcttctg gtggcatttg
     2161 ctgtacatga atcattagat cattaataat tgggtccggt tgtaatcctt cttcaacctc
     2221 cggctggttg gtaagtagtg ttttcttggt tttcgcggtt cgttttttgg ttgttgttcc
     2281 tttggacttg gttttggatt tctttttagt agatgccttt tctatgactt caaccggttt
     2341 cggtttgacg gtctttttct ttttcggaac atcgggagtt tccggggagg ctgttgcttc
     2401 atcattgacc ctatctttat ccattaattc tacctccttt cgatctgttc tgaacggtgt
     2461 agatttttac agggtatgaa tcaccgtccg aacaattttc atgaaaggtt ggcgtagatc
     2521 atacgccgga aaggtgatac cgcgcaaatg cgcgattcat tttgattaca ttatatgcat
     2581 gcctacggga atgtgcgatt tagccacgcg ggcccggagc cgcttcgacg atttatgccg
     2641 aaagcactat aaaaagaagg ctcccggtga agggagccat tcgtcgctat ccaatcgttc
     2701 aatttgagat ttttaccgaa ttaggatctg ggtgatcatt aagccgtacg gaccaccacg
     2761 gacgacccca actccaatcc gggtaaaatt gggattcaat agattactgc ggtgagccgg
     2821 gctttgattt tcagactgta gcgccttgat gcaactggat gcaccaatca gattttcggc
     2881 gacaatctga tacttgactc cggcatgttc caactgatcg taaaccgtgc cgaacctttc
     2941 ggaagaatgg ccgaaatagt tgaagcggac catatcccgg ctcttggttt cagccagttg
     3001 ttctaaggtg ggatcgacct gtaaatcggg taaaccagct ttcgctcttt cctggtttat
     3061 cagggaaacc atctccgcct cct
//
LOCUS       DASVMB010000132         6173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_310896,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000132 DASVMB010000000
VERSION     DASVMB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310896"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF531_04185"
     CDS             <1..403
                     /locus_tag="VF531_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7713198.1"
                     /translation="ALPMIRVDMGLIEQVLVNILDNAIKYSPPETELSLQARCQHNEL
                     LVSIRDRGEGVPSNEIGAIFDQFYRGKTSQAAKGTGLGLTICKAIINAHGGRIWAESA
                     EGQGTTITFALPISANSPKGIPEGNGLTSHE"
     gene            396..1079
                     /locus_tag="VF531_04190"
     CDS             396..1079
                     /locus_tag="VF531_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713199.1"
                     /translation="MSEKNCRILVIDDEPQIRRFLKVALTAHGFEMIEASLGGDGLNE
                     AVMSKPDLIILDMGLPDMDGLEVLKRLREWSQVPVIVLSVKEQEMTKIAMLDAGAEDY
                     VTKPFGMGELLARIRVALRRLSKDMEEPLVQLVDLRIDRAKRTVSVAERPIKLTPTEY
                     DLLKTLAASPGRVFTNTQLLKVVWGPGYEQETHYLRVFIGQLRRKIEEDPSQPIHLIT
                     EPGVGYRLM"
     gene            1232..1702
                     /locus_tag="VF531_04195"
     CDS             1232..1702
                     /locus_tag="VF531_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX7713200.1"
                     /translation="MVNRKQLLSKLNWFYSLELNQVDLYMAQSRAFKSSYESIVFERT
                     AWVEQHHVDNIGDIIKELGGRPTRLGSIVSPVIGRIAGKIISLFGIETTLKANIMIEN
                     KAMKDYTGLINTIRDEFGEELERILEHNLVDEDVHTAWFTERLFDYDNINLRNE"
     gene            1918..3804
                     /locus_tag="VF531_04200"
     CDS             1918..3804
                     /locus_tag="VF531_04200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7713201.1"
                     /translation="MRKIIILFFFIFLFSGIMLAADDTQQPISISSPVNPDAQLTEVD
                     NGSPSVTPMSTNPIRIVAPGENAHLPALSSTFVGGSVPVGGKLWINGAPVPVHPGGGF
                     LTMVNLSPGKFEIQAELQLGNDTYKVTRTVFVATPAQPTPVSPLTIEYVTPQQAQELL
                     PGDDVNVVCKGSPGMNAFFTVKGVRKKFPMIESQVAPGGMYYGLYRIGNKDRLKQSKI
                     KVTLINEKNQKVSKEATGTISLFQNRLPVMAETVSPDVILRAGPQLSPDNKAGYLLFP
                     PDGTLLQITGRIGDEYRVRLTKTKTVWVSSSQVRMLPEGTTPAPVVVGAVSVRADNHS
                     TIIRIPLGRKIPFAIDPDFEGDHVDLSFYGAFSNTDLITNPSAGIIKSIRWFQDDEET
                     YRLRVYTIPKGWWGYDARYEGTTFVLELRTPPPVTSNDSPLAGLSIAVDAGHSPDSGA
                     VGVTGYLEKDANLAQALNLKQKLQTKGADVIMIRKGDEGVPLGKRPQIAWQNRADILI
                     SLHNNSLPDGGNPFIKRGFGVYYFTPMSLALAKEVHTAYSEIFGPGSEFDLPDDGLYY
                     DNLALTRTPQMPSILVESAYMIVPEEEEYLKTDGFRSACANAIISGLERYVRMMRPGI
                     TKVN"
     gene            3907..5547
                     /locus_tag="VF531_04205"
     CDS             3907..5547
                     /locus_tag="VF531_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX7713202.1"
                     /translation="MRHFYRVMLIVIGLFVIVMGEDLSIKAEPIRPEVRAIWVDAFHD
                     GAKTPDQIDKLIKDCLTANINTLIVQVRRRGDAYYNQSIEPRTEDPKLPQGFDALQYL
                     IDKAHANHMEIHAWLNTLVAWNSSVPPKNPDHVWNQHGPKASARANWIAYYRTYDKTH
                     ASWSEKVYSSYFLDPGNPDALDYTVDVYLNVVKHYDVDGIHLDYSRYDGMGWGYNPTS
                     VARFNAQYGTTGLPAPDDPRWLQWRREQTANLVRKIYLKAIAIKPGLKVSSAVITWGD
                     GPVVESDWEKSRNYNDVAQDWLSWLREGIIDMVIPMNYFNEWNPIQQGWFNRWIEWEK
                     DHQYGRQIVIGPGIFSQYIEQSLDQIRRAQAPSALGNHTTGVALYAYAWDNLYSNDDF
                     RQPASAKILPRQPHVYMPETNEWLFPLLASPGSYLDPVLHQQIVTESVFSSPVPVPDM
                     PWKSHPFDGYLMGTVSGFGGKTYDHLLITVESANFGGSKISRQTFTDGSGWFGLSELP
                     PGEYFLRINHPDFAGQRPIFVGIQPGKVTEADVVPADR"
     gene            5848..>6173
                     /locus_tag="VF531_04210"
     CDS             5848..>6173
                     /locus_tag="VF531_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713203.1"
                     /translation="MKRIKIVIPVLLVFLLCFNVFAENKIVLNYWTQTDDNRTALENR
                     YIQEFEKANPNVEIKRVTNESSKMADLLLTAFSANNGPDLFNLTIDQEYAYMVNGRVA
                     PVDYRA"
BASE COUNT         1658 a         1363 c         1556 g         1596 t
ORIGIN      
        1 tgctctcccc atgatccggg tggatatggg gttaattgaa caagtattgg tcaatatatt
       61 ggataatgcg attaaatact cgccacccga aacggagctt tccttacaag cccggtgtca
      121 acataacgaa ctccttgttt caatccgcga tcggggcgaa ggagttcctt caaatgagat
      181 tggcgcgatc ttcgatcagt tttaccgggg gaaaacatcc caagccgcta aggggaccgg
      241 tttgggatta acgatttgca aggcgatcat caatgcccac ggaggtagga tatgggcgga
      301 gagtgcggag ggccaaggaa caaccattac ctttgcctta ccgatctccg ccaattctcc
      361 gaagggtatt cctgaaggaa acgggttgac aagccatgag tgaaaaaaac tgtcgaattc
      421 tggtcattga cgatgaaccg cagatccgtc gctttctgaa agtggcgctg acagcccacg
      481 gatttgaaat gatcgaagcc tcattgggag gggacggact caatgaagcg gtgatgtcca
      541 aaccggatct gatcatactg gatatgggac tcccggatat ggacgggttg gaagttctca
      601 agcggctgag ggaatggtcg caagtaccgg taatcgtctt gtctgtaaaa gagcaagaaa
      661 tgacgaagat cgccatgctg gatgcaggag cggaagacta tgtcaccaaa ccgttcggca
      721 tgggcgagct tctggcccgg atccgtgtgg cgttacggcg tttgtccaag gatatggaag
      781 agcctcttgt acagctcgtg gatctccgaa ttgatcgggc caaacgtact gtctccgtgg
      841 ccgaacgtcc cattaagttg acacccaccg aatatgatct cttaaaaacc ttagccgcaa
      901 gtccgggccg ggtttttacc aatacccaat tactgaaagt tgtctgggga cccggttatg
      961 aacaggaaac ccattattta cgggtattta tcggtcaatt gcgtcgcaaa atcgaggagg
     1021 atccctcaca gcccatccat ctaattacag aacccggggt gggatatcgg ttgatgtaaa
     1081 aggaaatggg agggacaagg atactttatt aagggtataa tccattttat gaaaaattca
     1141 tccatagctc cagttttatt caacagcatc tgcgggaata ttcgactatc cggttggaca
     1201 gtgtaaatat agaaaccagc tgacggagac aatggtgaac aggaaacaat tattatccaa
     1261 attaaactgg ttttatagcc tggagcttaa ccaggttgat ctatatatgg ctcagagcag
     1321 ggcatttaaa agtagttatg aaagcatcgt ttttgaacgc accgcctggg tcgaacaaca
     1381 tcatgtggac aatatcgggg atatcatcaa ggaattgggg ggaagaccga cccggttagg
     1441 cagtattgtt tcgccggtaa tcggaaggat tgccggtaag atcatttcct tattcggaat
     1501 tgaaaccaca ttaaaggcca acatcatgat tgagaataag gcaatgaagg attataccgg
     1561 tttaatcaat accatccggg atgaatttgg ggaggagctg gaaaggatcc tggaacacaa
     1621 tttggtggat gaagatgtgc atacggcgtg gtttacggag agactgtttg attatgacaa
     1681 tattaattta agaaacgaat agccataaag ggagaataga ggtttcccat tttagcttca
     1741 ctatctccag gccagtcgaa aaggaatcaa gactggcttt ttgattttac ctgaagattt
     1801 cattccattt tccttaaagg atacatttcc catgaaaaag atgaagggaa tgatcccaaa
     1861 aatgagaata aaaactaaag ccccaacaat cacctaaaga aagttgagga aaaactcatg
     1921 aggaagataa ttattttatt ctttttcata tttttattca gcggcattat gttggctgca
     1981 gacgatacac agcaacccat cagtatttca tcacctgtaa atccggatgc acagttaacc
     2041 gaggttgata atggctctcc ttcggtaaca cctatgtcaa cgaacccgat tcggattgtt
     2101 gcgccgggtg agaatgcaca tttacccgcg ttatcttcga cctttgtcgg cggttcggtt
     2161 ccggtagggg ggaaactttg gattaatggc gctccggtgc cggttcaccc gggtggcggc
     2221 tttttaacca tggttaatct ttcccccgga aaattcgaga tccaagccga acttcaactt
     2281 ggtaatgata cgtacaaggt cacccggaca gtttttgtgg ctacacctgc gcagccaaca
     2341 ccggtttcac cattaaccat tgagtacgta accccgcagc aggctcagga actgcttccc
     2401 ggggatgatg tgaatgttgt ctgtaaaggc agtcccggta tgaacgcctt ctttacagtg
     2461 aaaggcgtcc gcaaaaaatt tccgatgatc gagtcccagg ttgctcccgg tggtatgtat
     2521 tatggattgt atcggattgg aaataaggac aggctgaaac aatctaaaat caaagtaacc
     2581 cttatcaatg agaagaacca aaaagtatcc aaggaagcaa ccggcaccat ctcattgttt
     2641 cagaaccgcc tcccggtaat ggcggaaacc gtttccccgg atgtgattct gcgggccggt
     2701 ccgcaattgt ctccggataa caaggcaggg tatctcctat ttcctcctga cggtacattg
     2761 ctgcagataa cagggagaat tggggatgaa taccgggtgc gactgacaaa aaccaaaacg
     2821 gtatgggtaa gttcgagtca ggtgaggatg ctgccggaag gaacaacacc ggcgccggtt
     2881 gtcgttgggg ctgtttcggt tcgtgccgat aatcattcta cgattatccg tattccgctg
     2941 ggacggaaaa ttccctttgc catcgatccc gattttgagg gtgatcatgt ggacctctca
     3001 ttctacggcg ctttttcgaa taccgatttg ataacaaatc cttcagccgg tatcatcaaa
     3061 agtatccgct ggttccagga tgacgaggaa acatatcggc tgcgggtcta caccatccca
     3121 aaaggctggt ggggttatga tgcccggtat gaaggaacta cttttgtcct tgagttgcgg
     3181 acgccaccgc cggttaccag taacgattcc cccttggccg ggctttccat tgcggttgat
     3241 gccggtcatt cccctgatag cggtgcagtt ggggtgaccg gctatcttga aaaggacgcc
     3301 aatctagccc aggcgttgaa tctgaagcag aaacttcaaa caaaaggcgc cgatgtaatc
     3361 atgattcgta aaggtgatga aggggttcct ttaggcaaac ggccccagat cgcctggcaa
     3421 aatagggcgg atatcctgat tagtcttcat aacaactcgc tccccgatgg cggtaatccc
     3481 ttcatcaaaa gggggtttgg agtttattat ttcactccga tgagtctggc gttagctaaa
     3541 gaggttcata cggcttacag tgagatcttc ggcccgggca gcgaatttga tctaccggat
     3601 gacgggttgt actacgataa cctggcgctc acccgtactc cgcaaatgcc ttccatactc
     3661 gttgagtctg cctatatgat cgtccccgag gaagaggagt atctcaaaac agatggtttc
     3721 cgttcggcct gcgccaacgc catcatcagc ggtttggaac gatacgtccg catgatgcgt
     3781 ccggggatca ccaaggtaaa ctgaaacagt tgccaatgat tttttggtcc tgtgaacata
     3841 gccaagtttt ttccagaatc caatttttaa aagtagcacg agatgaaaaa cgattgggga
     3901 caattgatgc gtcattttta tcgggtaatg ttgattgtaa tcggtttatt tgtaatagtt
     3961 atgggtgagg atctgtctat caaggctgaa ccaatccggc cggaagtcag ggcaatttgg
     4021 gtggatgcct ttcatgacgg ggcaaaaact ccggatcaaa ttgataaact catcaaagat
     4081 tgtttaactg ccaacatcaa taccctcatc gtccaggtgc ggcgcagagg ggatgcttat
     4141 tacaatcaaa gcatcgagcc ccggaccgag gatcccaaac ttccgcaagg gtttgacgct
     4201 ttgcaatact taatcgataa agctcacgca aaccacatgg agattcatgc ctggctgaat
     4261 accttggttg cctggaatag ctcggttcct ccgaaaaacc ccgatcatgt ctggaatcaa
     4321 catggtccaa aggcttcggc cagagccaac tggattgctt actaccggac ttatgacaag
     4381 actcacgcaa gctggtctga aaaggtttat tcctcctatt ttctcgatcc ggggaacccg
     4441 gatgcgctgg attataccgt ggatgtctat ttgaatgtgg tcaagcatta tgatgtcgac
     4501 ggcatccacc ttgattattc acgctatgac ggcatgggtt ggggttataa cccgacgagt
     4561 gtagcccgtt ttaatgctca gtacggtact accggcttac cggcgccgga tgacccgaga
     4621 tggttacaat ggcgtcgtga acagaccgcg aatctggtta gaaaaatcta tctgaaggcc
     4681 attgccatca aaccgggttt gaaagtttct tcagcggtca tcacttgggg agatggaccg
     4741 gtcgtggaga gcgattggga aaaatccaga aactataatg atgtggcgca agactggcta
     4801 agttggctga gagaggggat catcgacatg gtgatcccga tgaattattt taatgaatgg
     4861 aatcccatac aacaaggctg gtttaaccgg tggatcgagt gggaaaaaga tcatcaatac
     4921 ggccgtcaga tagtgatcgg tccgggtatt ttttcacaat atatcgaaca atcgttggat
     4981 caaatcagac gggcccaagc gccgtccgct ctgggaaacc acacaaccgg tgtggccctc
     5041 tatgcctatg cctgggataa cttatatagt aacgacgact ttcgccaacc ggcatccgcg
     5101 aagattcttc ccagacagcc ccacgtctat atgccggaaa ccaacgaatg gctgtttcct
     5161 ttgcttgcga gtcccgggag ttatctggat ccggtactcc atcaacagat tgtcaccgaa
     5221 tctgtttttt catccccggt acccgtcccc gatatgccgt ggaaatctca tccgttcgat
     5281 ggttacttga tgggtacggt atcgggtttt gggggaaaga cttatgatca tcttctgata
     5341 acggttgaat ccgccaattt cggcggctca aagattagcc ggcagacttt taccgacggc
     5401 agcggctggt ttggtctctc ggaactgccg cccggagagt actttttaag aatcaatcat
     5461 ccggattttg caggacaaag gccgattttt gtcgggattc agccgggtaa agttacggaa
     5521 gccgatgtag ttccagcgga ccggtaattt caccggattc attattggtt tttttattta
     5581 tggagtcgcc attatcatgc ggatgccgtt ctttacgaaa atcccctttc ctacttctgc
     5641 cggaaatgca gtcggaggtc ttttgtgggg tattgtaacg gcggaaatag tcaaatggtt
     5701 ggacaataaa tatgtcgatg agtaaaggaa gttttatcgt tacaaagatg ttactttttc
     5761 gagaagattt tattacggtg ttttcctaga attgaattaa agagtaatac tctttgtatc
     5821 aacatcttta atgtgcggag gggaaaaatg aaaagaatta agatcgtaat cccggtgctc
     5881 ttggtctttt tactttgctt caatgttttt gcggaaaaca agattgttct gaattattgg
     5941 actcaaaccg atgataatcg gactgccctg gaaaacaggt acatccagga attcgaaaaa
     6001 gccaatccga atgtggagat taaaagggtt accaatgaat ccagcaaaat ggcggacctt
     6061 ctcctcacgg ctttttcggc caataacggt ccggatcttt tcaatcttac cattgaccag
     6121 gaatacgcat atatggtcaa cggccgggtc gcgccggttg attatagggc gat
//
LOCUS       DASVMB010000133         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_164146,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000133 DASVMB010000000
VERSION     DASVMB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164146"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VF531_04215"
     CDS             complement(<1..591)
                     /locus_tag="VF531_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7713204.1"
                     /translation="MLEYGVIASFSCLIYLLADQSWRRIQILTGENDKLREKIQLLSG
                     QLQKDNAYQDQLRYLSQLEERNRIAQNIHDKIGHTISGSLIQLEAAALYIDNDSAKAK
                     RIIEKVIPLLREGIDHIRITLKNIKPATEQLGINRIKLMLDEFTATNRVQPKLLYHGD
                     LERISQLQWRIIHDNLSEALTNVLKYAQASTVTVNIE"
     gene            complement(540..932)
                     /locus_tag="VF531_04220"
     CDS             complement(540..932)
                     /locus_tag="VF531_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713205.1"
                     /translation="MENWIVISKFIILAYIIGRLVENNPPTRNGLIILFLLIYICLNM
                     AFHLSKQKPAKQIWLGVSAILLSIGSRFLYSPVILLLPLNICELVYLYSNHPWLLITG
                     AFIPIFLVQKKPCWSMALSPLSAASSIF"
     misc_binding    1146..1449
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1495..2718
                     /gene="tyrS"
                     /locus_tag="VF531_04225"
     CDS             1495..2718
                     /gene="tyrS"
                     /locus_tag="VF531_04225"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7713206.1"
                     /translation="MDFIREQIRIIAKGAAEIINLDDLEQKLIKARQRGRPLTVKLGL
                     DPTAPDIHLGHTVVLRKIKQIQDLGHRAVIIIGDFTGMIGDPTGKSKTRKQLSRAEVI
                     DNARTYEAQILKVLDREKTEIRFNSEWLAGLNFVEVMELASKCTVARILERDDFHKRF
                     EVHESIGVHEFFYPLMQGYDSVALKADMEIGGTEQRFNILMGRNLQKDYGQESQVAVF
                     MPLLEGTDGQEKMSKSLGNYIGINENPDDIYGKVMSIPDSLIIRYFELATDIHPDELD
                     QIKEALVHQGVNPRDIKMRLAWEITSLYHGKVAADLAGEHFTSVFQRHAVPENIPERI
                     LPKELTGTGGTDIVKFMVDAGLTTSCSEAKRLIGQGAVRVNGQKIETFQITPLTDGDV
                     IQVGKLKFIRVITKE"
BASE COUNT          783 a          548 c          697 g          713 t
ORIGIN      
        1 ttcaatattg actgttacag tactcgcttg ggcatatttc aacacattgg tcaaggcttc
       61 actgagatta tcatggatga tccgccactg taattgggag atgcgttcca agtctccgtg
      121 atataaaagt ttgggttgga cccggttggt ggcggtaaac tcatccaaca tcaatttgat
      181 acgattaata cctaattgtt ccgtggccgg cttgatgttt tttaacgtaa tccggatatg
      241 gtcgattcct tcccgtaaaa gcgggatcac cttttcaata attcgtttgg ctttggcgct
      301 gtcattgtcg atataaagcg ctgcagcttc cagttgaatc aagcttccgg aaatggtatg
      361 accgattttg tcatggatgt tttgagcaat cctatttctc tcttccaact gggaaaggta
      421 gcgcagttga tcttgatacg cattgtcctt ttgtaactgg ccggataaaa gctgtatctt
      481 ctcccgtagc ttgtcattct cgccggttaa gatttggatc cgccgccaag attgatcagc
      541 tagaagatag atgaggcagc tgaaagaggc gataacgcca tactccagca aggttttttt
      601 tgaaccaaaa atatggggat gaatgcgccg gtaatcaaca gccacggatg attgctgtat
      661 aagtagacca attcacagat attgagcggc aggagcaata tgaccggtga ataaaggaac
      721 cgggaaccga tgcttaagag aatcgccgac accccgagcc agatttgttt ggccggtttt
      781 tgtttactga gatgaaaagc catgtttagg cagatgtata tcaataaaaa taagataatc
      841 aggccgttgc gagtcggtgg attattctct acaagtcggc cgataatata agctaaaatg
      901 ataaacttgg agataacgat ccagttttcc ataagtaaaa cctcttactg gatttaatcg
      961 ctagattttt ccttttgagt atatcatatt gtaccgatgg gaaaacagat caaggttttt
     1021 ttggtttcgt ttggctattt aataaacgca tactggttcg accgggatga taacttaaaa
     1081 accttcgtac agtagaaact atcttgacat aacgcatttt aaattgctat aataatccaa
     1141 ataatcgaat ggcgttgatg agaagaagta agaaagatcg gtcctgacag agagttcccg
     1201 gcaggtgaga ggggcgagga gatatctttc cgaatacctc tctgagccgc acaccaaaag
     1261 gctttgccga gtaggctgtt gccgggaacc tgcacccgtt atcgtgctag ggtataaata
     1321 cttgcaaacc actttgtctc tatatttagc ctggatgcaa attggttcgg tatcgtactt
     1381 gagaaggtta acgtcgcaag acgttgataa attgaggtgg taacacggga ttaaattccc
     1441 gtcctctggt aacccagagg gcgggaattt ttatttacaa aaggagggtt ttctatggat
     1501 ttcatccggg aacagattcg catcatcgcg aagggggcgg cggagatcat caatctggac
     1561 gacttggaac aaaaattgat caaagcccgt caaagaggca ggccgctaac ggtgaaacta
     1621 gggctcgacc cgacggcgcc ggatattcat ctgggccata cagttgtttt acggaaaatc
     1681 aaacaaattc aagacttggg acacagggcc gtcatcatta tcggcgattt taccggaatg
     1741 atcggcgatc cgaccggtaa atcaaagacg agaaagcaat tgagccgggc agaggtaatt
     1801 gacaatgccc gtacctatga agcgcagatt ctaaaggtac ttgacaggga aaagacggag
     1861 atcagattta atagcgaatg gctggccggg ctcaattttg tcgaagtcat ggagcttgct
     1921 tcaaaatgta ccgtcgccag aatactggaa agggatgatt tccataaacg cttcgaggtt
     1981 catgaaagca taggggtaca tgaattcttc tatcctttga tgcagggata tgactcggtt
     2041 gccttaaagg cagatatgga gattggcggt accgaacagc gttttaacat cctcatgggc
     2101 cgaaacctgc aaaaggatta cggtcaggag agtcaggtgg ctgtgttcat gccgctgctt
     2161 gaaggcaccg atggccagga gaagatgagc aaaagcctgg gcaattatat cgggatcaat
     2221 gaaaatccgg atgatattta cggtaaggtt atgtccatcc ccgacagtct cattatcaga
     2281 tattttgaac tggcgacgga cattcacccc gatgaactgg atcagataaa agaagccctt
     2341 gtccatcagg gagtcaatcc ccgggatatc aaaatgcggt tggcttggga aatcacctcg
     2401 ctctaccatg ggaaggttgc tgccgatttg gccggggaac attttacatc ggtatttcaa
     2461 cggcatgctg tcccggagaa tattccggaa agaatactgc ccaaggaatt gaccggtacc
     2521 ggcgggacgg acattgtcaa atttatggtg gatgcaggtt tgacaacaag ttgtagtgag
     2581 gcgaaaaggc taatcggaca gggagcggta agggtcaacg gccaaaagat tgagaccttt
     2641 caaataacgc cattgacgga tggggatgta atccaggtcg gaaaattgaa gttcatccgg
     2701 gtcatcacca aggagtgatt aatttggcaa caggcaacag g
//
LOCUS       DASVMB010000134         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_533814,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000134 DASVMB010000000
VERSION     DASVMB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533814"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /gene="pseG"
                     /locus_tag="VF531_04230"
     CDS             <1..1027
                     /gene="pseG"
                     /locus_tag="VF531_04230"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HEX7713207.1"
                     /translation="IGFRAEGGPGIGMGHIMRCLTLAQEFKASGHEVIFISNHRDGRE
                     TVTDAGFRTVTTGHGQESEAIAAIITSQHLDCLIVDSYRVDRDYFHRLKSILRLLCYI
                     DDLNRFIYPVDILINGNFGAETWGYQKYEPDEVLLLGTRYNLIRKEFQNLSPAQLKTA
                     VSRILITMGAADPVNFSARLIHGLRQDRTLDSVTLDVVVGPSNPFDCELQELSKSYPN
                     IRLHHQVRRMSELMLQADLAISAGGSTLYELCVCGVPTSAVIIADNQEFLVTGLTRSG
                     YIKSLGWHDQVDFGKLSESIRNVDISERWSLAAKGRKLIDGYGPGRVRQGIEIALGKR
                     RRVNFEV"
     gene            1271..1675
                     /locus_tag="VF531_04235"
     CDS             1271..1675
                     /locus_tag="VF531_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEX7713208.1"
                     /translation="MKVDMTNQPNNIVNADNKANTVNNVNPSSLNPDSAAARKPGVPE
                     ALNQSQETLKANTFPKPEDLEKSMNELKKKFNEIVDVGFEYSIDKDTNREVVKILDKR
                     TNEVIRQYPPEEILNMIKRMDEMIGFFVDERL"
     gene            1724..4321
                     /gene="fliD"
                     /locus_tag="VF531_04240"
     CDS             1724..4321
                     /gene="fliD"
                     /locus_tag="VF531_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX7713209.1"
                     /translation="MANNGLRIDGIVSGMDTQATIDKIMQASRAPLNRLQQKQQKLIW
                     QQEAYRTQNTALSKLRDMLLNLKLTSSYTTKTANSTDEKVATATANSRAGGSYRLEVE
                     ALAQSAQNQSQQSLSIRTTVTGGALSASTDAPIVIGDTNNSFSITVDGVPKNITITNG
                     NYASVKDLATNIQTQLNGAGFSEPVYVKAASDGSLMFHTGLKSDGKVRSITLNSVAGN
                     TGLDTLKFANGANSKELSGNAISSGKITINDSNKRFRISVGGLAVQEITLSGLSNGEH
                     TLQEVADEINTQLGALVSTNSEYGKVSVAVTSTNQLKFTYTNPADANNTAIKLQPGST
                     NDVLQELGFGTGTTSDAQRKTIDSSVSLWNQRDQFINSGFFADKTDDTKQQNGNFAFV
                     INGQAFSFTYRDTLDTIINTINGNAAAGVTAYYDEFSDKLVLNSTAVGDNNVGGAEIQ
                     LSDPNGILTQLFGIDQSKETGGTNAIIKLNGSTIQKTSNSFTINNVTFNLTGEGTTTV
                     TVGSNSEDVTKKVQDFVDKYNEIIGAMNTAVDEERATSGSKYTYYEPLTDEQKKEMSE
                     DQITAWEKMAKQGLLHNDSLLSDTLQQMRSSLSRMVTNPLTITGLPVADNVDLTGANH
                     FKVTFNGTTKEIVLDPTTYTRDDLKTALQNKFDLAFGTGAITVGSTGSGALKFTTNNI
                     SFTFNDADDQNALGQLGLGNGATVKVGYTALSQIGITTGDYTENGKLYFDQDKFKAAL
                     ANDADSVIRLLTNFDDPGIVATDSDTVKYQKTQKSELTKGIFYKLYDTVNQNINKILD
                     NVGSTGTINDSILGKQLSELGDQIDTKQDLLSEEEDRLWNQFTAMEQALSRLNSQSSY
                     LTSLLGGGS"
     gene            4491..4928
                     /locus_tag="VF531_04245"
     CDS             4491..4928
                     /locus_tag="VF531_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713210.1"
                     /translation="MSFQEIDELISRKLHLARELLDSSEELLKTSYSTERTSYEHFLG
                     KRAQCIEDLTQADILLKNQLNQIEPKDRPGLEERLANSDEQCRMILNRVLQIDQQNKA
                     GLMAEMAETKQKLALLRKGQQGRNAYANFGRVNSSGAFMDSRK"
BASE COUNT         1481 a         1187 c         1264 g         1180 t
ORIGIN      
        1 tatcggattt cgcgccgagg gcggccccgg gatcgggatg ggccacatca tgcgttgcct
       61 gacgctggcg caagagttta aagcgtccgg tcacgaagtg atatttattt ccaatcatcg
      121 cgacggccgt gaaacggtca ctgatgccgg attcagaaca gtaactacgg gccacggtca
      181 ggagtcggaa gccattgcgg cgattatcac ttcacaacat ttggactgcc tcatcgttga
      241 ctcttatcgg gtggatcgcg attatttcca tagacttaaa agtatcctcc ggttattatg
      301 ctacatcgac gacctgaacc gtttcatcta tccggtcgat atcctcatca acggcaactt
      361 cggtgcggaa acctggggtt atcaaaaata tgaaccggat gaggttttgc tcttaggaac
      421 ccggtataat ctgatccgca aggagtttca aaacctttcc ccggcccaac tgaaaactgc
      481 ggttagccgg atattgatca ccatgggcgc ggcggatccg gtgaattttt cggcccgctt
      541 aatccatggg ttgcgacagg atagaacctt ggattcggta acgttggatg tcgttgtcgg
      601 ccccagtaat ccttttgatt gtgagttgca ggaattgagc aaatcatacc ccaatatccg
      661 cctccatcat caggtgcggc gaatgtcgga actgatgctc caggccgacc tggccatatc
      721 ggcagggggc agcacccttt acgagttatg tgtttgcggt gtgccgactt cggccgtgat
      781 tatcgctgat aatcaagagt ttcttgttac aggtcttacc cggtcgggat atatcaagag
      841 tctgggatgg catgatcagg tcgactttgg gaaactgagt gaatccatcc ggaatgttga
      901 tatttcagaa cgctggagcc tggccgccaa agggcgaaaa ttgatcgacg gatatggtcc
      961 cgggagggtg cggcaaggaa ttgagatcgc tctcgggaaa aggagaagag tgaattttga
     1021 agtgtgaaac aaaacctgga aacttattga cttcatacta cttggatcaa ctgcttcgaa
     1081 aagcaacaca aagcgtattt attttattaa cgaatattta acaatctttt tcaacctgtt
     1141 atgcagctgt aatttttaaa accgataatc aaatattagg agaaaagtac tatatatttt
     1201 tccaaatatt ttcccgaaat aataggtaag gaaagctcca aaaaaatggg gttacaagga
     1261 ggtaaatgtg atgaaggtcg atatgacaaa tcagccgaac aacattgtta atgccgataa
     1321 taaagccaat actgtcaaca atgtcaatcc cagcagtctc aatccggatt cagccgcggc
     1381 aaggaagccg ggtgttccgg aggcgctcaa tcaaagtcag gaaacgctaa aagccaacac
     1441 ctttcctaaa ccggaggacc ttgagaaatc gatgaatgag ttgaagaaaa aattcaacga
     1501 gatcgtcgat gtgggcttcg aatactccat cgataaggac accaaccggg aagtcgtcaa
     1561 gatcctggat aaacggacca acgaagtgat tcgccaatat ccgccggaag agatcctgaa
     1621 tatgatcaaa agaatggacg agatgattgg cttctttgtg gatgaacgct tataataagg
     1681 gattcgtttt gaattcgtct gactttataa ggaggttttg atcatggcca ataatggttt
     1741 gcgaattgat ggaatcgtat ccggcatgga tacccaggcc acaattgata agatcatgca
     1801 ggcgtctaga gcgccgctta accgattaca acagaagcaa cagaaactca tctggcaaca
     1861 ggaagcatac cggactcaga atacggcgct atccaagctc cgtgatatgt tattgaatct
     1921 gaagctgact tcttcctata caaccaagac cgccaattcc accgatgaga aggtggctac
     1981 ggccacggcc aactcccgcg ctggcggcag ttacaggctg gaagttgaag cattggccca
     2041 atccgcccag aatcaaagtc aacagtcgct gtcaatccgg acgaccgtca cgggcggggc
     2101 attatcagcg tcgactgatg ctcctattgt tattggtgat acgaataatt cattctccat
     2161 tacagtagac ggtgtcccaa aaaatatcac gattaccaac ggtaattatg cgtcggtaaa
     2221 ggacctggca accaatattc agacccagct caatggggct gggtttagtg aaccggtcta
     2281 tgtaaaggcg gcctccgacg ggagcctgat gtttcacacc gggttaaaat cggacggtaa
     2341 ggttcgtagc atcaccctga attcagttgc gggcaatacc ggtctggata ctttgaaatt
     2401 cgccaacggc gccaacagca aagagctgag cggtaacgcc atcagttcgg gaaagatcac
     2461 cattaacgac tccaataaaa gattcaggat ttccgtaggc ggcctggcgg tccaagagat
     2521 tacgctatcg ggtctgtcca acggcgaaca taccttgcag gaagtcgccg atgagatcaa
     2581 tacacaattg ggggcacttg tctccaccaa ctcggaatac gggaaagtaa gtgtggctgt
     2641 aaccagtacc aaccaactga agttcaccta taccaatccc gccgacgcga ataacaccgc
     2701 gatcaaactg caaccgggct cgaccaacga tgttttgcaa gagctgggtt ttgggaccgg
     2761 aactacatcc gatgcgcagc ggaaaaccat cgactccagc gtttccctat ggaaccagcg
     2821 ggatcaattc atcaactccg gcttttttgc ggataaaacc gatgacacca aacaacagaa
     2881 cggcaatttc gcattcgtca tcaacggcca ggcgttttcc tttacctacc gggatacgtt
     2941 ggataccatt atcaacacga ttaacggcaa tgccgccgcc ggggtcacgg cctactacga
     3001 tgaattcagc gacaagctgg tgctcaactc cactgcggtc ggagataaca atgtaggcgg
     3061 cgccgagatc caattaagcg atccgaacgg tattttaact caattgttcg ggatcgatca
     3121 atcgaaggag accggcggga ccaacgccat aatcaagctg aatggctcaa ccattcagaa
     3181 aacgagtaat tcctttacga tcaacaatgt gaccttcaac ttgaccgggg aaggaaccac
     3241 gactgtcacc gtaggcagta attccgagga tgtcaccaag aaggttcagg atttcgttga
     3301 taaatataat gagatcatcg gcgccatgaa taccgccgtt gacgaggaac gcgccacttc
     3361 cggcagtaaa tatacctatt acgagccctt aaccgatgaa caaaaaaagg aaatgagcga
     3421 ggatcagatt accgcctggg agaaaatggc caaacagggc ctcttgcata acgactcctt
     3481 gctctcggat acgcttcagc agatgcgatc gagtttgtcg cggatggtta cgaatccgtt
     3541 aaccattacc ggtctgccgg tagctgataa cgtcgatttg accggggcga accactttaa
     3601 ggtgaccttt aacggaacga ccaaagagat agtcctggat ccgacaacct atacccggga
     3661 cgatttaaaa accgcccttc aaaacaaatt cgaccttgct tttggaacag gggcgataac
     3721 ggttggcagt accgggagtg gagccttgaa attcaccacc aacaacatct cgtttacgtt
     3781 taacgacgcc gacgatcaaa acgccctggg tcagttggga ctgggcaatg gggctaccgt
     3841 taaggtcggt tacaccgcac tcagccagat cggaatcacc accggcgact ataccgaaaa
     3901 cgggaagctc tattttgacc aggataagtt caaagccgct ttggccaacg atgcggacag
     3961 cgtcatccgg ctgcttacca actttgatga tccgggcatc gtggccacgg acagcgatac
     4021 tgtcaaatat caaaaaaccc agaagtcgga actgacaaaa ggtatttttt ataagttgta
     4081 tgatacagtc aaccagaata tcaacaagat attagacaat gttggatcca ccggtactat
     4141 caatgacagt attctcggaa aacagttatc ggaattagga gatcagatcg acacgaagca
     4201 agacctactg tccgaggagg aagaccggct ttggaaccag tttaccgcca tggagcaggc
     4261 gcttagcagg ctgaattcgc aatcatccta tcttaccagt ctattgggcg gaggcagctg
     4321 atcgctgaga aggaaaacag tataaggcaa caggcaacag ggaataggca acagtaaaat
     4381 taccggtaac gaaagacatt tagtttctgt tgcctgttgc caagtgcctg ttgcctcact
     4441 aagccattct tgagtcgacc attttaagtt taaggaattt ggtgatccca atgagttttc
     4501 aggaaatcga cgaattgatc agccgtaagc tgcatttagc ccgggaatta ctcgatagtt
     4561 ccgaggagtt gcttaaaacg agttattcca cggaaaggac cagctatgaa cattttttag
     4621 ggaaaagggc tcaatgtatc gaagatctta cgcaagccga tattttatta aagaaccagc
     4681 taaatcagat tgagcctaag gatcggcccg ggcttgagga acgtctggct aatagtgatg
     4741 agcagtgtcg gatgattttg aaccgggttt tacaaatcga tcaacaaaac aaagccggct
     4801 taatggcgga gatggcagag accaaacaaa aattggcgct gcttcggaag ggtcaacaag
     4861 gccgaaacgc ttatgccaat ttcggccggg tgaattcatc cggggctttt atggatagcc
     4921 ggaaataggt aacaggcgca ggcatgaggc atgagtcgaa agactaaagc caaagttatc
     4981 tgggcataga acaggcagta gatctcatgc ctcgagcctc atgcctttca agaacaactt
     5041 acttttttat tcgggaggga tccagaatgg ctctaaataa ggcctattca gcttatcaga
     5101 caaattcgat tt
//
LOCUS       DASVMB010000135         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_93243,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000135 DASVMB010000000
VERSION     DASVMB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93243"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VF531_04250"
     CDS             <1..1109
                     /locus_tag="VF531_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HEX7713211.1"
                     /translation="QNLLDPGKTPHENEQFLLFLSAVIQAVDEYSDILRASAANTGND
                     HRLGANEAPPAIISVYLGEQLADIFAQLEENGGADHSKSGGELKVGVTTLPTLRKDST
                     DRNRTSPFAFTGNKFEFRMVPSSASIATPNTTLNTIVAETLSRIADRLEKAKDIDAEV
                     PKIIKEIVKDHKRIIFNGNNYSEEWLKEAEKRGLPNIRSTVEAVKVWGSPKAITLFEK
                     HHVLSKVELHSHYEIYLENYSKQINIEALTMLDMAKRQILPAILKYTGEVAQSLNQIK
                     TASAKANVVAFEDLLHTLTETTAAFNRNLTALEKALNEAEKENEDSLKIAEYFRDVVF
                     AAMGRLRVEADRLETMVDAKVWPFPTYADMLFNV"
     gene            1308..2342
                     /locus_tag="VF531_04255"
     CDS             1308..2342
                     /locus_tag="VF531_04255"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX7713212.1"
                     /translation="MYPLKFTPVYQERIWGGTGLALEFGRKLPSDRVGESWEISAHPN
                     GLSRVGNGPLAGESIPQLIGDFGTAFLGVKAPSQAFERFPLLIKLLDASEKLSVQVHP
                     GNAYALEHEGELGKYEIWYILAAKPGAKIVYGLKTGTDKAAFEEALACGDLESCLNEV
                     PVKAGEVYAIPPGLVHALGAGIMVAEIQQNSDTVYRVYDYNRVDQSGKTRALHLQKAL
                     DVIRFDDFLPDHPLAPLPNSRLIDSEFFKTDYCVTSAADTSVNGTRDDVINENSTGSE
                     PQKHYYNDGSRFYILTNLSGKAQLCSEATAESGRICLEWERGESLLIPADLTEFAING
                     SVSFLKTYLP"
     gene            2453..>2574
                     /locus_tag="VF531_04260"
     CDS             2453..>2574
                     /locus_tag="VF531_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX7713213.1"
                     /translation="MKVKVIKKTERQRQLLAAIEQNPFITDEELAETLKVSIPT"
BASE COUNT          692 a          613 c          657 g          612 t
ORIGIN      
        1 gccaaaatct gctggatcca ggcaaaactc cgcatgaaaa cgagcaattc ctactctttt
       61 tgtccgccgt gattcaagca gtagatgaat attccgatat tttaagggct tccgcagcca
      121 ataccgggaa cgaccatcga ctgggagcca acgaggctcc gccggccatt atctcggttt
      181 atctcggcga acaattggcc gacatcttcg cccaactgga ggagaacggc ggcgccgatc
      241 attccaagtc cggcggagag ttgaaagtag gggttacgac cctgcccaca ttacgcaagg
      301 attcgaccga ccgtaaccgg acatcgccgt tcgcctttac cggaaataaa tttgaattcc
      361 ggatggttcc ctcttcggct tccatcgcta ctcccaatac cactttaaac acgattgttg
      421 ccgagacatt gtcgcggatc gccgaccgtc tggaaaaggc caaggatatc gacgccgaag
      481 ttcccaagat tatcaaagaa attgtgaaag atcataaacg cattatattc aacgggaaca
      541 actactccga agaatggttg aaggaagccg aaaaacgggg tttacccaat atccgttcca
      601 cggtggaggc cgtcaaggtc tggggcagcc cgaaagccat cactctcttc gagaagcatc
      661 atgttttaag caaggtggaa ctccattccc attatgagat ttacttggag aactatagta
      721 aacaaatcaa cattgaagcc ttgaccatgc tggacatggc aaaacgtcaa atcttaccgg
      781 caattttgaa gtatacggga gaggttgccc aatcactcaa ccagatcaag accgcctcgg
      841 cgaaagccaa tgtggtggca tttgaggatt tgctgcatac tctcactgaa accacggcgg
      901 cctttaaccg gaatcttacc gctttggaga aagcattgaa cgaagccgaa aaagagaacg
      961 aggattcatt gaaaatcgca gaatatttcc gggatgtggt ctttgccgct atgggccgtt
     1021 tgcgggttga ggccgatcgt ctggagacaa tggtggatgc gaaggtttgg cctttcccga
     1081 cttatgcaga tatgttgttt aatgtttaat gtttcgtcaa tgaataaatg gattgagccc
     1141 gggaggtttt cccgggtttg ttttttgtac gcccggcacg ggcgttatca cactcgcgaa
     1201 gcttgacgga atgaaaaggg tctgctagaa tttgggtatt agtcgcgccc gctcgcgtgg
     1261 cacaaagccg atggaaaagg aaccggcgct atttgaggtg aacaatcatg tatccattaa
     1321 aatttacccc ggtttatcag gaacggatct ggggcggtac cgggcttgca cttgaatttg
     1381 gtcgaaaact gccatcagac cgtgtcgggg agagctggga gatttccgct catcccaatg
     1441 gcctcagccg ggtcggtaac gggccgctcg cgggggagag tattccccaa ttgattggcg
     1501 atttcggtac ggcatttttg ggtgtcaagg ccccgtctca ggcattcgaa cgcttcccct
     1561 tgcttatcaa gctccttgat gcgagcgaaa agctatctgt ccaggttcat ccaggaaatg
     1621 cctatgcctt ggaacacgag ggcgagcttg gtaaatatga gatttggtat atcctggccg
     1681 caaaacccgg ggctaaaatc gtttatggat taaaaaccgg aaccgataag gcggcatttg
     1741 aggaggcact ggcctgtgga gacctggagt cctgcttaaa tgaggtaccc gtcaaagcgg
     1801 gagaggttta cgcgattccc ccggggctcg ttcatgcact gggcgcaggg attatggtgg
     1861 cggagattca gcaaaattcg gatacagttt acagggtata cgactataac cgcgtggacc
     1921 aaagcggaaa gacccgcgct ctccatcttc agaaagcttt ggatgtgatt cgcttcgatg
     1981 attttctacc ggatcatccc ctagcacccc ttcccaatag ccggttaatt gattcggaat
     2041 ttttcaagac ggattactgc gtcacgagtg ccgccgacac aagtgtcaac ggaactcgtg
     2101 acgatgtcat aaatgaaaat tccaccggtt ccgagcctca aaagcattat tacaatgacg
     2161 gcagccgttt ttatatccta accaatctct ccggaaaagc tcaattgtgt tccgaagcga
     2221 ctgccgagtc agggcggata tgtttggaat gggagcgggg cgaatccctg ttgatccccg
     2281 cagatcttac cgagtttgcc atcaacggtt ccgtgagctt cttaaaaact tatttaccgt
     2341 agaatatatg gcaacttacc cccgattagg cttatctcga aaataacttg cgaaggggat
     2401 aggaatcggt tataattatt atcaggtaat aataaaaagt agcggcgaaa agatgaaagt
     2461 gaaagtcatt aaaaaaacag aacggcaacg ccagttactc gcggcaatag aacagaatcc
     2521 ctttatcacc gacgaggaac tagccgaaac cctaaaggtc agtattccga cgat
//
LOCUS       DASVMB010000136         7905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_385779,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000136 DASVMB010000000
VERSION     DASVMB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385779"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF531_04265"
     CDS             <1..415
                     /locus_tag="VF531_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX7713214.1"
                     /translation="YAKLFIGTRYRYGGDSPNGFDCSGFTMFVYSHYGFKLPHQAASQ
                     INLGTEVSREQLIPGDLLFFKTLGSTRVNHVGIYLGEGQFIGASSGHGSVTIAPLNEG
                     YYATRFCGARRLIDLPDETDPTDTEDQTVAETIGA"
     gene            complement(602..1030)
                     /locus_tag="VF531_04270"
     CDS             complement(602..1030)
                     /locus_tag="VF531_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713215.1"
                     /translation="MPLVNYNRSLLSNFANSSTFVLTAANQLVLEFGLFVPQTPNFVE
                     LLTTAGWTVTNVSPVEPNQPTLMLTITQDGVPVATSDQESIEDRGDEPLENLATFQAV
                     LTNVPTGFHVYRLFARNIQPAQGTITITGPTNISAKVITV"
     gene            complement(1297..2382)
                     /gene="hydE"
                     /locus_tag="VF531_04275"
     CDS             complement(1297..2382)
                     /gene="hydE"
                     /locus_tag="VF531_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HEX7713216.1"
                     /translation="MAKLLSQIPESIRQEIDILLGDRERLLELIAKPDIPEAREILKL
                     ADQVRIWTVGDEVQLRGLIEFSSFCRNHCLYCGLRQDNRKLVRYRLEAPEIITAAENG
                     VRLGYKTVVLQSGEDPSYTGEGLAEVIREIKNLGLVVTLSLGEREPWEYELWRKAGAE
                     RYLMRHETSAPALYARLHPAGRTLEKRVALLKVLKGLDYQVGTGFMVGLPGQTPETLL
                     ADLELARELNAEMVGVGPFIPHPETPLGGTVGGTVAQTCLMVALVRLILPWALVPATT
                     ALGTIDPLGREAALQSGANVVMPNLTPRTYRKDYQIYPNKICTGEEAAECRSCINRRI
                     TAIGRSVAAGPGHNVLWLERQRGNKKS"
     gene            complement(2397..2660)
                     /locus_tag="VF531_04280"
     CDS             complement(2397..2660)
                     /locus_tag="VF531_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HEX7713217.1"
                     /translation="MTINLEKRLGVIAVIIEDPPEIQAKVNELISASGRIVIGRMGIP
                     HWERNIATLALIVEGTENEINTLTGSLGNLPGVSARATMAKKS"
     gene            complement(2700..3923)
                     /gene="hydF"
                     /locus_tag="VF531_04285"
     CDS             complement(2700..3923)
                     /gene="hydF"
                     /locus_tag="VF531_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HEX7713218.1"
                     /translation="MNQTPSSSRLHIALFGRRNAGKSSLLNALTHQQAALVSAEPGTT
                     TDPVAKAMELLPLGPVVFIDTAGLDDRGSLGEMRVEKSYEVLNRTDLAVLVLDGPTGL
                     TDFDIHLLENVRFKGIPVVGVINKQDIVDDLPEKRREWEAKLGLELIEVSAVSGQGLD
                     ALKMALVHNASVDDLKQVLTGDLLRPGDLAVLVVPIDKAAPKGRLILPQQQVIRDILD
                     HNALALVTKETELQAALNFLDKKPALVITDSQAFAMAAAHTPREIPLTSFSILLARQK
                     GDLQQLVRGALAIKELRPGDRVLIAEGCTHHRQSDDIGTVKIPRWLRQTVGGELEFEW
                     TSGLTLPQDLHRYRLVVHCGGCMLNRREMLYRLSLAREQGVPMVNYGVLIAYVQGILP
                     RVLEPFPEVREIIGE"
     gene            complement(4181..5629)
                     /gene="hydG"
                     /locus_tag="VF531_04290"
     CDS             complement(4181..5629)
                     /gene="hydG"
                     /locus_tag="VF531_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HEX7713219.1"
                     /translation="MGFLEKYQKDFAEYDAMNQDFIDDEEIWRLLHKWEHPKVTDVRM
                     VLEKSARQVRLDPEEMAILLQNHDLETVEEMYALANQLKREVYGDRIVFFAPLYISDQ
                     CANDCLYCGYRRSNPAIHRKTLTMKEIRREVEIMIDEGQKRTVLVYGESPQTEIGFLC
                     DSVRQVYSVKSKHGEIRRANINCAPLRREELRELKRVGIGTFQVFQETYHHETYRRLH
                     PADTIKGHYRWRLYCHDRALDAGVDDVGIGVLFGLYDWRFEAMGLLYHAIHLEKKYNG
                     VGPHTISFPRIQPALGTPFSHHPEGAVSDEDFKKLVAVMRLSVPYTGMILTARESAAV
                     RREVIPLGISQIDAGSRIGVGGYRKSLANAMPDKEQFQLGDTRSLDEVIRETCQMGSI
                     PSLCTACYRSGRTGENFMGLAKSSFVHNYCIPNALCTFKEYLLDYASQETRNIGEQVI
                     HEYLTQLPENKSKEFVEQALVKLERGERDLYI"
     gene            6013..6972
                     /locus_tag="VF531_04295"
     CDS             6013..6972
                     /locus_tag="VF531_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX7713220.1"
                     /translation="MPVFDLPLAQLQKYQGKNPCPPDMDSFWDQSLEEMKRVDPQVEL
                     MPASFQTSFAECFDLYFTGVRNARIHAKYLRPKNSPGPHPAILQFHGYSANSGDWNDK
                     LNFVALGYSVAALDCRGQGGLSEDTGGVKGNTLNGQIIRGLDDAPENMLMRHIFLDAT
                     ELARIVMDMAEVDPHRVGAMGASQGGGLTLACAALEPQIKRLTPMYPFLSDYLRVWEM
                     DLATGAYAELREYLRRFDPMHLREQEIYTRLGYIDIQHLAKRIRGEVLMATGLMDTIC
                     PPSTQFAVYNKITAPKKLVVFPDYGHEGIPCFWDEAYQFFTGL"
     gene            7531..>7905
                     /locus_tag="VF531_04300"
     CDS             7531..>7905
                     /locus_tag="VF531_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713221.1"
                     /translation="MKTTETIVLETSSRKSPTLDYLKKYWTLYLMMLLPITYFVIFRY
                     IPMTYIQIAFKKFSLVQNVWQMPWADNGGFEYFIKAFANRDFLYALRNTIMLNLLDLL
                     IGFPAPIILALLLNELPFKRLKS"
BASE COUNT         1895 a         2089 c         1874 g         2047 t
ORIGIN      
        1 ttacgccaag ctttttatcg ggactcgtta cagatacggc ggggactccc caaacggctt
       61 cgactgctcg ggttttacga tgttcgtata ctcgcactac ggtttcaaac taccacacca
      121 ggctgcctcc caaatcaacc tggggacgga ggtcagtcgt gaacaactca ttccggggga
      181 tcttttattc tttaagacct tgggatcgac ccgggtcaac catgttggta tctatctcgg
      241 agaaggccag tttatcgggg cttcctctgg ccacggcagt gtaacgattg ccccattaaa
      301 cgagggatat tacgccaccc gcttctgcgg cgcccggcgg ctcatcgatc tgccggatga
      361 gacagatccg acggatacag aggatcaaac ggttgcagaa acaattggcg cttgaaaatt
      421 tggctgtggg tgaaagcatg taaaccggcg taatgaacga gtcggggctg ccaattctta
      481 ggtaaaaaag gaccggcact gatacaatgt gccggtcctt ttttctctta ttgcaggtta
      541 gtgagtgagt ttaggccgac gttgagtgga tatttaacac agactgccgt cccctgcttt
      601 tttaaactgt aatgacttta gcagaaatgt tcgtaggccc agtgatggtt atggttcctt
      661 gagcaggctg aatgttacgg gcaaataagc ggtaaacgtg gaaaccagta ggtacattcg
      721 ttagaacggc ttggaatgta gccaaattct ctagcggctc atcccctcga tcttcaatcg
      781 actcttgatc cgatgtggcc accggtacac catcttgagt aatcgtgagc attagagtag
      841 gttgattcgg ttccaccgga ctgacgtttg ttactgtcca accagctgtt gttaacaatt
      901 caacaaagtt cggtgtttgg ggtacaaaca gaccaaattc caaaaccagt tgattggcag
      961 cggtgagcac aaaagtggag gaatttgcaa aatttgataa gagactccga ttatagttta
     1021 cgagaggcat ctatttccct cctttgattt gggtggatgg gagtcgacaa tatttacctt
     1081 tttacgcttt agttctattg aataggtcca attgtctaac tcccatattt gggttggaat
     1141 attgtattgt attgttcata ttcaattttg gttacctttt ttagtcatat tcatatttaa
     1201 tcctcagaga cttccgaaaa cactgatgtt ttctggccct tttagtgtaa accttaaact
     1261 tttcgagaat gtgcgaatat atgactgttc cactttttaa ctttttttat ttcccctctg
     1321 tctttccagc caaagtacat tatgacccgg tccggcagcg acgctccggc cgatagcggt
     1381 gatcctccgg ttgatgcaac ttcggcactc cgcggcctct tccccggtgc agattttgtt
     1441 gggatagatc tgataatcct tgcggtacgt acggggggtc aagttgggca tcaccacatt
     1501 agcgccggat tgaagcgctg cttcccggcc caacggatcg atggttccca gagccgtggt
     1561 ggcgggaaca agagcccagg gaagaatcag ccggaccaaa gccaccatca ggcaggtttg
     1621 ggctactgtt cccccaaccg ttccgcccaa gggagtctcc ggatggggaa tgaaagggcc
     1681 gactcctacc atctccgcat tcaattcccg cgccaattcc aaatcggcca gcagtgtctc
     1741 cggggtctga cccggcagcc ccaccatgaa gccggtaccc acctgataat caaggccttt
     1801 taagaccttg agcaaggcca cccttttctc aagcgtccgg cctgccggat gcagcctggc
     1861 gtaaagtgcg ggagcggatg tctcgtgacg catcaggtaa cgctccgctc cggccttccg
     1921 ccataactca tattcccaag gttcccgttc gccgaggctc aaagtaacca ccagaccaag
     1981 gtttttaatc tcccggatca cctcggccag gccttcacca gtatatgacg gatcctcacc
     2041 ggactgcaaa accacggtct tatacccaag cctgacgccg ttctcagccg ctgtgataat
     2101 ctccggcgct tcgagccggt agcggacgag ctttcggtta tcctgtcgca gcccgcagta
     2161 gagacagtga ttccggcaga agctggagaa ctcaatcaac ccccggagct ggacctcgtc
     2221 tcctacagtc caaatgcgga cttggtccgc taacttcaaa atttcccggg cttccggtat
     2281 atcgggcttg gcgatgagct caagcaaccg ttcccgatcc ccgagaagta tatcaatctc
     2341 ctgtcgaatg ctttcaggta tttgcgaaag taacttcgcc attttaactc catcttttat
     2401 gatttcttgg ccatggtcgc ccgggcgctc actccgggaa gattgcccag actaccggtg
     2461 agggtattga tctcgttctc cgtaccctct acgatcaaag ccagtgtggc aatgttccgc
     2521 tcccaatgag ggattcccat ccgtccgatg acgatccggc ccgaagcgct gatcagctcg
     2581 ttgaccttgg cctggatctc cggaggatct tcaataatca ccgcaatgac ccctaaacgc
     2641 ttttctagat tgatagtcat gataatctct ccttttagta ggcataaggc atgaagcttc
     2701 tactcaccaa ttatctctct cacttccggg aacggctcaa gaacccttgg aagaatgcct
     2761 tgaacatacg cgatcaacac gccgtaattg accatcggta caccctgctc ccgggcaagc
     2821 gataaacggt acagcatctc gcggcggttc aacatacatc cgccacagtg gaccaccagc
     2881 cggtagcgat gcagatcttg gggtaatgtc aggccgctcg tccactcgaa ctctagctct
     2941 ccgccgacag tctgccgtag ccagcggggg atcttgaccg tgccgatatc gtcggactga
     3001 cgatgatggg tgcagccctc ggcaatcaag acccggtcac ccgggcgcaa ctctttgatt
     3061 gccagagcgc cccggaccag ttgctgcaag tcgccctttt ggcgggcgag caagatggag
     3121 aacgaagtaa gaggaatctc acgcggtgtg tgggccgcag ccatggcaaa agcttgtgaa
     3181 tcggtgatga cgagtgccgg ttttttatcc agaaaattca aggccgcctg caactcggtc
     3241 tccttggtca ccaaagccaa ggcgttatga tccagaatat cccggatcac ctgctgttga
     3301 ggaaggatca atctaccctt gggcgccgcc ttgtcaatgg gcaccaccaa aaccgctaaa
     3361 tccccgggtc gcaataagtc gccggttaaa acctgtttca gatcatcgac cgaagcgtta
     3421 tgaaccagcg ccatcttaag cgcatcgaga ccttgtccgg aaaccgccga tacctcaatc
     3481 agttcaagtc cgagttttgc ctcccactcc cggcgctttt cgggtaaatc atcgacaata
     3541 tcctgtttgt taatcacccc gactaccggg atccccttaa accgaacatt ttccagcaaa
     3601 tgtatatcaa aatcggttaa acccgtaggg ccgtccagca ccagaacggc aagatccgta
     3661 cggttcaata cctcataact tttctccacc ctcatctcgc ccaaggaacc ccggtcatcc
     3721 aaaccggcgg tatctataaa gaccaccggc cccaggggaa gtagctccat ggctttggcg
     3781 accggatcgg tggttgtccc gggttcggca gagaccagcg cagcttgctg gtgagtgaga
     3841 gcattcaaca aacttgactt cccggcgttc cttcggccga acaatgcgat atgaagccgc
     3901 gaagaactgg gggtttgatt catattatcc acggtattct cctttaatag gaaacaggga
     3961 acaggcacta ggcaacagat gttagaaatg aggtagatgt ccctaggtta accctatcag
     4021 gctattacct attgtctatt agcaactcac ccgcgcgtcc ccgcaggacg cgatgcctta
     4081 tgccttttgc ctgttgcctg tcgcttttga atagactcgc tcttcctgaa acgccctact
     4141 catgccctac gccttttgcc tattgcctat tgcctgttcc ctaaatataa agatccctct
     4201 ctccccgctc aagttttacc agcgcctgct ccacgaattc tttcgactta ttctcgggta
     4261 attgggtcag atactcgtgg atcacttgtt ccccaatgtt ccgtgtctcc tggctggcat
     4321 agtctaaaag gtattccttg aaggtgcaaa gggcgttggg aatgcaatag ttatggacaa
     4381 agcttgattt ggcaaggccc atgaagtttt ctccggtccg gcccgatcgg taacaggcgg
     4441 tgcagagcga aggaatgctc cccatctgac aggtctcccg gatcacctca tccagggaac
     4501 gggtatctcc cagttggaac tgttccttat ccggcatggc gttggctaaa gacttccggt
     4561 aacctcccac cccaatccgg cttccggcat cgatctggga gatccccagc gggatcactt
     4621 cccgccggac cgcagcgctt tcacgggcgg tcagaatcat cccggtatag gggaccgaca
     4681 atcgcattac tgccacaagc tttttaaaat cttcgtcact taccgcccct tccggatgat
     4741 gggagaaagg tgtgcccagc gccggctgga tcctggggaa tgagatggta tgggggccga
     4801 ctccgttata cttcttttcg aggtgaatgg catggtataa aagccccatc gcctcaaatc
     4861 tccagtcata caggccaaac aatacgccga tgccgacatc gtctactccc gcgtcgagcg
     4921 cccggtcatg acaataaagc cgccaacgat agtgtccttt aatggtatcg gccggatgca
     4981 gccgcctgta tgtctcgtga tgataagtct cctgaaagac ctggaatgtc ccgatgccca
     5041 ccctttttaa ctcccgcaac tcttcacgcc ttagtggggc gcaattgatg ttggctcggc
     5101 gaatctcgcc atgcttactt ttgacactat aaacctggcg tacgctgtcg cagagaaacc
     5161 cgatctccgt ttggggtgat tcaccgtaga ccagaactgt ccgcttttgc ccctcgtcaa
     5221 tcataatctc cacttcccgg cggatctctt tcatggtcag cgtcttgcga tgaatggccg
     5281 gattggatct gcggtagccg cagtaaagac aatcattggc acattggtcg ctgatataga
     5341 gcggcgcgaa gaaaacgatc cggtcgccgt acacctcccg tttcaactgg tttgccaagg
     5401 catacatttc ttcaaccgtt tccaaatcgt gattttgaag cagtatcgcc atttcctccg
     5461 gatccagccg gacctggcgg gctgatttct ccaaaaccat acgaacatcc gtaaccttgg
     5521 gatgctccca tttatgtagg aggcgccaaa tctcctcgtc gtcgatgaag tcttgattca
     5581 tggcatcgta ctccgcgaaa tccttttggt acttctcgag aaaacccatc catcccacgc
     5641 tccttttggt ctttaaaaaa caaaaaccct tctttgatcc gccggaaggc atctcaaaaa
     5701 agggtatgag ccgaaagcgc ccttaaagag cgcgggatca accatttatg aaagtgctct
     5761 ccggccttag actgttcttt ggcctcagaa catcagtttc gcttgattca tcaggatgac
     5821 gttaaccccg attaaaatca cccatgacaa acctttattg taatattagc acattgtgaa
     5881 aaaaagaaca atcctctttt agagaattta tgaagacgac tgcttcagat ctcgttttgt
     5941 tttgtcttgt tacaggaaat aactgttata cggcgaaagg atcctcactc attatcaaag
     6001 gggatgattt ttatgccggt cttcgatctg ccactggccc aacttcagaa gtatcaagga
     6061 aaaaaccctt gcccgccgga tatggattca ttttgggatc agtccctgga agagatgaaa
     6121 cgggttgatc ctcaagtgga gttgatgccc gcttcatttc aaacttcctt cgccgaatgc
     6181 tttgatcttt atttcaccgg agttcggaac gcccggattc atgccaaata tctccgtccc
     6241 aaaaacagcc ccgggcctca cccggccatt cttcaatttc acggctactc agccaattcg
     6301 ggggactgga acgataaact gaactttgta gccttagggt attccgtggc cgccctcgac
     6361 tgccgcggtc agggcggact ctccgaagat accggaggag tcaagggcaa caccttaaac
     6421 ggccagatca tccggggcct ggacgacgct ccggagaata tgctgatgcg ccatattttt
     6481 ctggatgcta cggaattggc acggatcgtc atggatatgg cggaggttga tccccaccgg
     6541 gtcggagcca tgggtgcttc ccagggaggc gggttaaccc tggcctgcgc ggcactggaa
     6601 ccgcagatta agaggttgac gccgatgtat ccgtttctca gtgactacct gcgggtttgg
     6661 gaaatggatc tggcaaccgg cgcctatgcc gagttgcgtg aatatttaag gcgctttgat
     6721 ccgatgcatc ttcgtgaaca agagatttac acccgtctgg gctatatcga tatccagcat
     6781 ctggccaagc gaatccgagg cgaggttttg atggcaacgg gtcttatgga tacaatctgc
     6841 ccgccttcga ctcaatttgc ggtttacaac aagattaccg ctcccaaaaa gctagtggtc
     6901 ttccccgatt acggccatga gggaattccg tgtttctggg atgaagctta ccaattcttt
     6961 acgggtttgt aaaagacacg gattacggga tattgcttaa aaatcagccg taatcctgaa
     7021 tcaatgaaca tatttccatc aaaaaccttc ctttcttttt tactttatat taagaccgta
     7081 gttacaaaca gttcggcatg tgctatacta ataataggtc agcctagatg aagtttcttc
     7141 tatatacaat tattatcaat aattttgccc gagtgatatt gccactgcat gtggaaatga
     7201 ctcgggtaga ttacgggtcg ctaagggtta cttttttccg ggaacgttcc cggaaaaaat
     7261 tgagatgatt tgcacgaggg aatcgctgtc gaaattttaa gaacaacaag ggttttaccc
     7321 ctgttgatag ttagaagttt tattgaattt catcttttga acaaatattt gtttcttaat
     7381 tgtatattgt attcccgatg aacgtttgtt atcaagcgtt tagaaggaag gatagccaac
     7441 atatattttg aataatgtga aatagattaa tagttgtgcg caatgtaagc gttttctgtt
     7501 tcatatttaa agaacaaacg gggtgcgata atgaaaacaa ctgaaacaat tgtgctggaa
     7561 acgtcgtcca gaaagagccc gactcttgac tatttaaaaa aatactggac gctctatctc
     7621 atgatgcttt tacccatcac ttatttcgtg atttttcgct atataccgat gacttatatc
     7681 cagatcgcgt tcaaaaagtt cagcctcgtc caaaacgttt ggcaaatgcc atgggcggat
     7741 aacggaggct ttgagtattt catcaaggct tttgccaacc gcgacttcct ctatgcgctg
     7801 cgtaacacca tcatgttgaa cctgcttgac ttgttgatcg gctttcccgc cccgattatc
     7861 ctggcgctgc tcctcaacga gctgcccttc aaacggctca agagc
//
LOCUS       DASVMB010000137         8787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_164494,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000137 DASVMB010000000
VERSION     DASVMB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164494"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(224..385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(463..1371)
                     /locus_tag="VF531_04305"
     CDS             complement(463..1371)
                     /locus_tag="VF531_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7713222.1"
                     /translation="MNVHQIIPSDRAVPRHQALLFLIGAALLWSSGGLLIKMVVWHPL
                     AIAGMRSAIAAVFMIIWRRGVRFNWSPAQIYGALAYAATVILFVIANKMTIAANAILL
                     QYTAPVYTAILAGLLLKERTSRLDWITISIVIGGMALFFLDRLSFRGLWGNACAILSG
                     VAFASEAVFLRMQKDGSPLESIILGNILAALIGLPFMFGSAPDASGWLGLVLLGLFQL
                     GVSYILYATAIKSVTALEAIIIPIIEPILNPIWVFLVFQEQPGRWAFLGGTIVLITVT
                     VRCILVALKENHSKSVNPSNRGSYDS"
     gene            complement(1479..2330)
                     /gene="dapF"
                     /locus_tag="VF531_04310"
     CDS             complement(1479..2330)
                     /gene="dapF"
                     /locus_tag="VF531_04310"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7713223.1"
                     /translation="MEFYKYHALGNDYIVIDPQHAPDLLLTPERIRLICHRNFGVGSD
                     GILYGPLFEDGQIRLRIFNPDGSEAEKSGNGIRIFSKYLVDAGYLSKNQFSLDTAGGR
                     VAVELLNTQATLIRVNMGVVTFWSERIPVTGSPREVVNEPLSLPGGVFRVTCLSLGNP
                     HCVVPLPNISKELACELGPSIENHPQFPKRINTQLVQVLDRHNIRIEIWERGAGYTLA
                     SGSSSSAAACAAYKLGLVDPELTVHMPGGTITIAILPDWHVLMSGSVSYVARGQLSAE
                     FQSDINR"
     gene            2753..3682
                     /locus_tag="VF531_04315"
     CDS             2753..3682
                     /locus_tag="VF531_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7713224.1"
                     /translation="MKRALVLSGGGSMGAFEVGAVDYLVHKAGYDFNVLMGTSVGALN
                     TAFLGQAWSYQELLKATGDLKNFWLQIKGDTSIYQKSILGYLKLLFRDALYEPKGLKA
                     LLKAQVDPERICRNPKRIVKVATVAMETGELLYADNRDPRYRPEFLNYVLASASMPLF
                     FPGAPIDGKHWYDGGLRDITPLGAVFQESPDEIVIIITFPIGPDLSPRLPQVKPKGAI
                     GALLRTIDILTGEIEANDLQLARMINDKAEYFPECRKIPLRIIAPVAPLESNGPLDFN
                     PQKIRHNMERGYEAARDSRLIIPSNRNAVGRSR"
     gene            complement(3709..5124)
                     /locus_tag="VF531_04320"
     CDS             complement(3709..5124)
                     /locus_tag="VF531_04320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7713225.1"
                     /translation="MMLRKGYIGLILLPFLGWVLPVPFLDGAGLLAVLIYSLCYGRGG
                     GLFAAVWSALVTTVMYILQSSIPLELHLAGIIMFFPVAWGTGWAMDYARRKQLAFEEM
                     EDQLRYLGMHDPLTKIYNRAYFELESERLEKARPDSVGVLVCDVDGLKLINDTLGHEA
                     GDKILVLACNLLKEVVGDQGSISRIDGDEFVILLPNLDQTGVEEISRGIRESEAHHNQ
                     HNTELSLSISMGSAVTRQPNLNIHQLFKEADNNMYREKLHRFHSTRSTLVQTVTSMMK
                     ARDLVTEDHALRLMDLITWLARLLGMSERSITEMSLFAEFHDIGKVGIPDRILFKEGV
                     LTPPEIRQMQRHCEIGYRIARSSRDLTAIADWILKHHEWWNGTGYPLGLKGEEIPFEC
                     RVLAVVDAYDAMTNDRPYRKAFTHREALGELRRCAGVQFDPFVVERFAAMLESEGKIH
                     MFPDRGPVAIKSDRKTEIPGF"
     gene            complement(5351..>8787)
                     /locus_tag="VF531_04325"
     CDS             complement(5351..>8787)
                     /locus_tag="VF531_04325"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX7713226.1"
                     /translation="RTAKLIILEGSITDAEFQRIKTYCINPVESREATLAKPASLTQE
                     IRVPDNVTSITGFIRMNRLELEGLHRQMNLAMSIDDLAFCQEYFRDTEGREPTIAEIR
                     VIDTYWSDHCRHTTFLTRLESVAFEPGDFSGLMEQAYREYRHSRELIYHGREKAESLM
                     DLATIAMKELKLNGKLDDLDESDEINACSILVTALVDGVPEEWLVMFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRAYVYQAMRVTGSADPRKPVTETLQGKLPQRKITNGAA
                     AGFSSYGNQIGLATGQVTEVYDQGYLAKRMEIGAVIGAVPRANVRRETPQPGDTVILL
                     GGRTGRDGCGGATGSSKAHDEESLLTCGAEVQKGNPPTERKLQRLFRNPVVSRMIKRC
                     NDFGAGGVAVAISELADGLRVDLDAVPKKYEGLDGTELAISESQERMAVVVAPGDCAG
                     FIDAAREENLEATPVATVTAEPRLRMIWREAAIIDISRAFLNTNGVPQTIRVEVASPN
                     GKREFFRSLPQDIADAPHPEEAWLRNLERLAVCSQKGLAERFDSTIGAGTVLMPFGGS
                     RQLTPAEGMAAKLPIQRGDTTTGTLMTFGYNPVIAKWSPFHGAVYAIIEAVAKIAAVG
                     GDYRQIRLTLQEYFEKPGDDPKRWGKPFAALLGAYYAQMRLGIPAIGGKDSMSGSFND
                     LDVPPTLVAFALAVADVRRVLSPEFKLAGSQVVLLPLKRDGAELPDFEQLGRNCSRVY
                     QLIGSGKIRSAISVKTGGLAEALTKMAIGNMIGCKLIVQPEMNQWFAPDYGSLVLEIP
                     PEVAIDQAFEGLDYRLLGVTVAEPRITAGGLRIDLETARSRWEAPLEPVFPMDIAALT
                     DMKPPRSHNYQPQYSVKPHRNLARPRIVIPVFPGTNCEYDSARAFEKAGGLPELLIFR
                     NLSPWEIEASVELLVRKINNAQIIMLPGGFSGGDEPDGSGKFIATVFRNPYVKEAVTR
                     FLEERDGLMLGICNGFQALIKLGLVPFGSIRDITEDSPTLTYNTIGRHVSCLVRTRVA
                     SKLSPWLGKTQLGEIHSIAVSHGEGRFVASPALLEQLAKSGQIATQYVDEAGNPSYDR
                     RYNPNGSFEAIEGITSPDGRIFGKMGHSERYGPGVAVNIPGSKDQPIFRAGVEYFK"
BASE COUNT         2272 a         2362 c         2071 g         2082 t
ORIGIN      
        1 tttcaccgtt catagcaata agatccttga tttttccaaa ggttctgccg tccggcaact
       61 gtagtcggtg agccaggagc ctgctgagat aaaaactctt gatggcggcc atgggaagtc
      121 cccccctttt acctcgtata acctacattt gcaacccaag aaaatcggga cggacaaaaa
      181 aataaagctc tcaccaaagc cggacgtggt gaaagctcaa aagtcgttca ctcccgttcg
      241 agctttggca ctgcaaggca gtgaagatgc attaggtaat gccttgtttc gtttcggcaa
      301 aacctgctaa cctgctcagg ttgtctccaa ccatctgcgg tagcatcccg tatccttgca
      361 ggaacctcgc ctaccgagta ttcctttagg tttatattag cactggaact aagtaagttc
      421 aacctctttt actatttatt tacgtgattt gactgctata tattatgaat cataactccc
      481 ccggttactc gggttaacgc tttttgaatg attttccttt aaagcgacca gaatgcaacg
      541 gacagtcacg gtgattaaaa cgattgttcc acccaagaat gcccagcgcc cgggttgttc
      601 ttgaaaaacc agaaagaccc aaattggatt aagaatcggt tcaataatcg gaataatgat
      661 agcctccaag gccgtcacgg atttgatggc cgtggcgtat aaaatatagg atacccccag
      721 ttgaaacagt cccaaaagca ctaggcccaa ccaccccgag gcgtccgggg ctgaaccgaa
      781 catgaatgga agaccaatca aagctgcgag aatatttccc aggataatgg attcaagagg
      841 cgaaccgtcc ttctgcattc tcaggaatac cgcctcactg gcaaaggcta cgccggagag
      901 gatggcacag gcattccccc ataaaccacg gaaagaaaga cgatccaaaa agaaaagggc
      961 cataccgcca attacgatag agatggtgat ccagtcgagt cgggaggttc gctccttcag
     1021 caataacccg gccaggatgg cggtataaac cggggcggta tactggagta ggatagcatt
     1081 tgccgcaatg gtcattttat tggcgatgac gaacaggata acggtggccg cgtaggccag
     1141 cgcaccatag atctgggccg gggaccaatt aaaacggaca ccccgccgcc aaataatcat
     1201 gaagaccgct gcaatggcgc tgcgcattcc ggcaatcgcc agaggatgcc agacgaccat
     1261 tttaatcaat agaccgccgc tgctccataa aagtgcagcc ccaatcagaa acaacaatgc
     1321 ctgatgtctg ggaaccgccc tgtcacttgg aatgatttga tgaacattca tgatttggtc
     1381 tcttccttta tcttgttgtt cgattctatc ggcacccact ggatacctat agagccaaac
     1441 cgcggattat caaggtttat ccgagagcat gaatctatct accgatttat atccgattgg
     1501 aactcggcac tcagttggcc ccgggcaacg taagagacag agcccgacat caagacgtgc
     1561 caatcgggaa gaatcgcgat ggtaatcgtt cctcccggca tatgaacggt gagttcagga
     1621 tccaccaaac ccagtttata cgcggcgcat gccgcggcag aactgctact gcccgaggct
     1681 agagtataac cggcaccccg ttcccagatc tcgatccgaa tattatgccg atccaatacc
     1741 tgaaccaact gagtattaat acgcttggga aactgaggat gattttcgat cgatggtcca
     1801 agttcacaag caagttcctt ggagatattg ggcaagggta ctacacaatg gggatttccc
     1861 agtgacagac aagtcacccg gaagactcca ccgggcaagg aaagaggctc attcacgact
     1921 tcccggggtg aacctgtcac cgggatacgc tcgctccaaa aagtgactac tcccatatta
     1981 acccggatca gcgtagcctg agtatttaaa agttctactg cgaccctgcc gccggccgta
     2041 tctaaagaaa attggttttt tgaaagataa ccggcatcca ccaagtactt ggagaagatc
     2101 cggataccgt taccgctttt ttcggcctca ctaccatccg gattgaagat tcgcaaccgg
     2161 atttgtccat cttcaaataa cgggccatat aaaattccat cggaacccac gccaaaatta
     2221 cgatgacaaa tcaaccgaat cctctccggg gtcaataaca gatctggggc atgttgcgga
     2281 tcaatcacaa tatagtcgtt acccagggcg tgatatttat aaaattcgat cactttatca
     2341 aacctccgta gggatgaatt cctgaaaata ttcgcccttt tcaacaaccc acctgccgat
     2401 tggcaaaaaa aaagccgcgg tcggtgacca cagctttcaa aaaggaggaa gttaaatgaa
     2461 aaagtttagt attactataa cacatgtttt tgttaatctc ataacagacc tgtaaaggat
     2521 tggttaagaa ctctagctgg aattacgatc actcccccac ttaaaacagt ttcaaaactg
     2581 cacaaaccag ctgcaatgct taatagaagg gtgaaagtga agtggagaat taaaaccaaa
     2641 acagaactta aataaatgtt gaaattaata atgaactcag gtagactgaa aattagaaac
     2701 cggtttatgt gagaaaaaga acgcattctt attaacgatg gagggtttta agatgaaaag
     2761 agccttggtt ttgtccggcg gtggttccat gggtgctttt gaagtaggcg ctgttgatta
     2821 cttggtacat aaggcaggtt atgacttcaa cgtactcatg ggtaccagcg ttggagcact
     2881 aaataccgcc tttctgggcc aggcatggag ctatcaggag ttgctaaagg ccaccggcga
     2941 cttaaagaat ttttggctgc agatcaaagg tgatacctcc atctaccaaa aaagcatcct
     3001 gggttacctc aagttattat ttcgggatgc cctttatgaa cctaaaggat tgaaggctct
     3061 tttaaaagcg caggttgatc cggagcggat ttgccggaat cccaaacgga ttgtcaaagt
     3121 cgctacggtc gccatggaaa ccggggaact gctctatgca gataatcgtg acccgcggta
     3181 cagaccggag ttccttaact atgtcctggc aagcgccagt atgccgctgt tctttccggg
     3241 agccccgatc gacgggaaac attggtatga cgggggatta agggatatta ccccgctggg
     3301 tgcggttttc caggagtcac ccgatgagat cgtgatcatt atcacctttc ccatcggtcc
     3361 cgatttatcg ccccgcttgc cgcaggtcaa gcccaagggt gccatcgggg cgttattacg
     3421 gacgattgat atcctgaccg gcgaaattga agccaatgat ttgcagttag cccggatgat
     3481 taacgacaag gccgaatatt tcccggaatg ccggaagata ccactgcgaa tcattgcacc
     3541 ggtcgcacct ttagaatcca atgggcccct ggattttaat ccgcaaaaaa tccgccacaa
     3601 tatggaacgg ggctatgaag ctgcccgtga ctcacggctc atcatcccca gtaaccgaaa
     3661 cgccgtaggt cgatcgcgct agttactagc cggcaagttc aatctttctt aaaaccccgg
     3721 gatttccgtt ttccgatctg attttatggc caccggtcct ctgtcgggga acatatggat
     3781 tttaccttcc gattccagca tggcggcaaa acgttcaacc acaaacggat cgaactgtac
     3841 cccggcgcaa cggcgcagtt cgccaagggc ctcccgatgc gtaaatgcct ttcgataagg
     3901 gcgatcattg gtcatggcgt catatgcgtc aactacagcc aggacccggc attcaaaagg
     3961 aatttcctcc cccttaagac ccaggggata accggttcca ttccaccact cgtggtgttt
     4021 caagatccaa tcggcaatag cagtcaaatc ccttgaggac cgtgcgattc ggtacccaat
     4081 ctcacaatgc cgttgcatct gcctgatttc aggaggcgtt aatactcctt ccttaaagag
     4141 gatccgatcg gggataccca ccttaccgat atcgtgaaat tcggcaaaga gactcatttc
     4201 ggtaatgctt cgttcggaca tgccaagtaa tcgagcgagc caggtgatga ggtccatgag
     4261 ccggagggcg tgatcttcag tgaccaaatc acgtgctttc atcatgctcg tcacggtttg
     4321 taccaaggtg ctccgggtac tatgaaagcg atgaagtttt tcccggtaca tgttgttatc
     4381 ggcttctttg aagagttgat ggatgttaag attcggctgc cgggtgacag ccgaacccat
     4441 ggagatgctc agggataact cggtgttatg ttgattatga tgggcctcgc tctcacggat
     4501 tccccggctg atctcctcaa ccccggtttg atccaggttg ggaaggagaa tgacaaactc
     4561 atcaccatca atccgggaga tgcttccctg atcacctacg acctctttca aaagattaca
     4621 agccaagacc agtatcttgt caccggcttc atgacccaaa gtatcattga tcagcttcaa
     4681 accgtccaca tcgcagacga gcactccaac cgagtcaggc cgtgcctttt ccagacgttc
     4741 cgattccaac tcgaaatagg cccgattgta gatcttggtc aatggatcgt gcattccgag
     4801 ataacgtaac tgatcctcca tctcctcgaa cgccaattgt ttccggcggg cgtaatccat
     4861 ggcccaacct gtaccccagg cgactgggaa gaacatgatg ataccggcta aatggagttc
     4921 aagcggtatg gatgattgaa ggatatacat cacggtagtt acaagcgcac tccaaaccgc
     4981 cgcgaataaa ccgccacccc ggccgtaaca caggctgtaa atcaaaaccg ccaaaagtcc
     5041 cgctccatcc agaaatggaa ccggtaaaac ccagccgaga aatggcaata gaattaaacc
     5101 tatatagccc ttccgtagca tcataggcag tatcggacgt tccctccctg gtgaattatt
     5161 gaaatctctt acttttttgg tctattcatt ataatttttt ctttcaactg tttatataat
     5221 attcgctctt ttttggattt ttcctgcaca tttcaccagg tatccaacga atttacccct
     5281 gcttaccatt gataaaacta taaaaaaaat cagagatttt ttctctgatt tcacttctct
     5341 agtggttgac ttatttaaaa tattccactc cggcacgaaa aatcggttga tctttggaac
     5401 ctggaatatt cacggctacc cccgggccgt aacgttccga atgccccatc ttaccgaaga
     5461 tccggccatc agggcttgtt atcccctcga tcgcctcaaa cgatccgttg ggattgtaac
     5521 gcctatcgta gctggggttc ccggcctcgt ccacatactg ggtagcgatc tggccgcttt
     5581 ttgccaattg ctcaagcaag gcaggactcg ccacaaaacg tccttcgcca tgagagaccg
     5641 cgatcgaatg aatttctccc aattgcgttt tgccgagcca tggagagagc ttggatgcaa
     5701 ctctggtcct taccaggcaa gatacatgcc ttccaatggt gttataagtc aaagtcggac
     5761 tatcctctgt tatatcccgg atggagccaa aaggtaccaa tcccagtttg ataagggcct
     5821 gaaacccatt acagatcccc agcatcaaac cgtcccgttc ctcaaggaac cgggtgactg
     5881 cttctttaac atagggatta cgaaataccg tagctataaa ttttccggaa ccgtccggtt
     5941 cgtccccacc gctaaaacca ccgggcaaca tgatgatctg ggcattatta atcttccgta
     6001 ccaataactc aacggaggcc tcaatctccc aaggagacaa attccgaaag atgagcaact
     6061 ccgggagccc gcccgctttt tcaaacgccc gcgccgaatc atactcgcaa ttggtaccgg
     6121 gaaaaaccgg gatgacaatc cgggggcggg ccagattccg atgaggctta accgaatact
     6181 ggggttgata attgtgtgac cggggtggtt tcatatcggt caaagcagca atgtccatcg
     6241 gaaacaccgg ctcaagaggt gcctcccacc ggctacgcgc agtttcaaga tcgatccgaa
     6301 gcccgccggc agtgatccga ggttcggcga ccgtcacccc aagaagcctg taatcaagcc
     6361 cttcaaaagc ttgatctata gcgacctccg ggggaatctc cagtaccagt gaaccgtaat
     6421 ccggcgcaaa ccactggttc atctccggtt ggactatgag cttgcaacct atcatattgc
     6481 cgattgccat tttggtaaga gcctccgcca gtccaccggt tttgaccgaa attgccgaac
     6541 ggatcttgcc ggaaccgatt aactgataaa cccgcgagca attgcggccc aattgttcaa
     6601 aatcgggcag ctccgcgcca tcacgtttca gagggagcag caccacctga ctgccggcca
     6661 gcttaaactc gggcgaaagc acccggcgca catcggcaac agccaatgca aacgccacta
     6721 acgtgggagg gacgtccaaa tcattaaagc tgccgctcat actgtcctta ccgccaattg
     6781 cgggaatccc cagcctcatc tgggcatagt aggccccaag caacgccgca aagggtttgc
     6841 cccaacgttt cggatcatca cccggctttt cgaaatattc ctgtaaagtc aggcgaatct
     6901 gacggtaatc gccgcccacg gccgctatct tggctacggc ttcaatgatg gcatagaccg
     6961 cgccgtggaa cgggctccat ttggcgatca cggggttata gccaaaggtc atgagggtgc
     7021 ccgtagtggt atcaccccgc tgtatcggaa gtttagcggc cataccctca gccggggtta
     7081 attgccggga gccgccgaag ggcatcaaaa cagttcccgc accgatagta ctgtcaaatc
     7141 gttccgccaa tcctttctgg ctacataccg ccaaccgctc cagattacgg agccaggcct
     7201 cttccggatg cggcgcatcc gcgatatcct ggggcaggct acgaaaaaac tcccttttcc
     7261 cgttgggaga agccacttcc acccggatgg tttgggggac tccgttggtg ttgagaaagg
     7321 cccggctaat gtcgatgatt gctgcttcac gccagatcat tcgcagccgt ggttcagcag
     7381 tcactgtggc aacgggtgta gcctccagat tttcctcccg ggccgcatcg atgaatccgg
     7441 cgcaatcccc gggagccact actactgcca ttcgctcctg ggactcggaa atcgccaatt
     7501 ccgtcccgtc gaggccttca tatttcttgg gtactgcatc gagatccacc cgcaggccgt
     7561 ccgccaattc actgatagcc acggccactc cgccggcgcc gaagtcgtta cagcgtttaa
     7621 tcatccggct gaccaccgga ttgcggaata acctttgcag ttttcgctcc gtgggcggat
     7681 tacctttctg aacctcggca ccgcatgtca ataaagattc ctcgtcatgg gctttggaag
     7741 aacccgtagc acccccgcag ccatcacgtc cggtacggcc acccaataaa atgacggtat
     7801 ctcccggctg cggcgtctcg cgacggacgt tcgcccttgg aacggccccg atgaccgcgc
     7861 caatctccat tcgtttcgcc agataaccct gatcataaac ctccgttacc tgccctgtgg
     7921 caagtccaat ctgattgccg taggaactga aaccggctgc ggccccgttg gtaatcttcc
     7981 tttgcggtag tttaccctgg agggtctctg taacaggttt tcggggatcg gcgctccctg
     8041 tgacacgcat cgcctgatag acataagccc ggcccgacaa tgggtcgcga atggctccac
     8101 ccagacaagt ggctgctccc ccaaagggtt cgatctcggt cggatggtta tgggtttcgt
     8161 tcttgaacat taccagccat tcttcgggaa cgccgtccac taaagcggtg accaagatac
     8221 tgcaggcatt gatctcgtcc gattcatcca gatcatccaa tttgccattc agtttaagtt
     8281 ctttcatggc gattgtggcc aagtccatga gggattctgc tttttccctg ccgtgataaa
     8341 taagttcccg cgagtggcgg tactcccgat aggcttgttc catcaaaccg ctgaaatctc
     8401 cgggctcaaa agccacagat tctaggcgtg tcaaaaaagt ggtatgtctg caatgatccg
     8461 accaataggt atcgatcacc cggatctcgg cgatggtcgg ttcccgtccc tccgtatccc
     8521 gaaaatactc ttgacagaat gccagatcat caatagacat ggcgagattc atctgccgat
     8581 gcaaaccctc aagctcaaga cggttcattc gaataaaacc ggttatagag gtgacattat
     8641 caggaacccg gatctcctgg gtaagactcg ccggcttggc cagtgttgct tcccggcttt
     8701 caaccggatt gatacagtac gtcttgatcc tttgaaattc ggcatctgta atcgagcctt
     8761 ccaggatgat taatttagct gtccggg
//
LOCUS       DASVMB010000138         4669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_681026,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000138 DASVMB010000000
VERSION     DASVMB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681026"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3497
                     /locus_tag="VF531_04330"
     CDS             <1..3497
                     /locus_tag="VF531_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7713227.1"
                     /translation="TAGAGTGKTSVLVGKYMQLLVERRAQVGEIVAITFTKKAAAEMR
                     ERIRLGLQQHLERAGDTEADFWKSQLSQVETARISTFHSFCLGLIREHPLEIGIPPMV
                     GILGEGEATIYLKQSIETVLAEVFRGQNQDNSELFRILLEFGWDSFETSLAGLYETIR
                     ESGIPFDEAEKRSRIELKAAIAAIPESVTELSEAVSELLEYSRDAALTERAMELMAAL
                     RESWPALREAITNGAVLDETLPALSELSEALPRNQPNSIKERVGAIRDMAEGLSRKLL
                     NQEALCRLPAFREILERVDAAYRRLKQDLGCLDFTDQLILARDLLRNHPDLAEEIRGS
                     LCYLLVDEFQDTNGLQMELVQLLWGETHPGGRFMAVGDIKQSIYRFRGAEADLILKLA
                     DELREATQGRLISLSRNYRSSGRVIQFVNALSMPLFMGEPFTYEPLLADEPQGTAAVE
                     FLLTGDFDRRIEAKLVGKQIQQLVSSVPDRETAWHYGDIVLLFRTKTAIPLYQKVFQE
                     MGIPYYSASSSGFYRCLEVTDQLNLLRVTLQRYDGVALMALLTSAYVGLNDECLFWLG
                     TDEGAHARDLVGRFYAADFPAELPTLARYKLGQFRELLIYLQEHRDLLGIPGILRAAL
                     ERTHYREMLQIMPNSIQRQANLDKLLAKADEFVAKGFHDLHRFLDYLEKLETAEVIEG
                     EASTQAEAGDVVRLMTIHRAKGLEFPVVIIPDLDRQFSRGLRGRLAFHKNTGIGFTIR
                     YGDGENGAPSHWEQLKALNRREEIAELKRLLYVALTRAKQLLILAGSGCNSSKAKTIE
                     TADNWMKWFQLLLPLEGAGAELDWEGVPLRIIRDLPASEVPPKAQLLIEAHRAELSTG
                     IPGATAQAEMEIASTALAGQERSLTLKVTEVLTFKSCSRRYFWKYRMRFPEDPAPIEG
                     WDSEVKGPSSPAVAEQAVTLEAQGIVSPVATGSAERSYGSAYVLRSGESVRGLGTRIG
                     TFVHQAIRGVASEWPETLWSEVFYDLPGPDKEQLKRDLQQIWRNFRNSPFTEQAGQFW
                     DEVPFRIKLGEKLKMEGRFDRLLMSPDGKLILVDYKTHRSSAAKVPELAKGYLWQLQL
                     YAMAVKALWGRLPDRAVLYFIYSNQTFSVPLTPEALRGMESEIREIGQFIAVHDRAAD
                     YPSADDCKGCGYSWCCKL"
     gene            complement(3473..4309)
                     /gene="folE2"
                     /locus_tag="VF531_04335"
     CDS             complement(3473..4309)
                     /gene="folE2"
                     /locus_tag="VF531_04335"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX7713228.1"
                     /translation="MIDVQNMRDERGIEIQRVGIKDVHLPLQIRMKDGGFQHVQGNVS
                     ASVNLPQEFKGTHLSRFMELLTSWSEKPISGKEIRQILAELCHHLNAAEAEFSLKFRY
                     FLPLKAPVSSAVGSLDYIGEFRGNLIEQGHCFDYQLGVEVPVLSLCPCSKEISAFGAH
                     NQRAAIRALVRFGYPGKILWLEDLISILRIQGSCPVYPVLKREDEKFVTESAYQNPKF
                     VEDILRDSVLALRSEPRINWFRVEVESYESIHNHNAFATHMEDCSSGQAAVEHHSLQH
                     QE"
BASE COUNT         1100 a         1182 c         1314 g         1073 t
ORIGIN      
        1 ttactgcagg agcaggtacc ggcaagacca gcgtcctggt agggaaatat atgcaactac
       61 ttgtggaacg ccgggcgcag gttggtgaga tcgttgccat caccttcacc aaaaaagcag
      121 cagccgagat gcgggaaagg atccgccttg gattacagca acatttggag agagccgggg
      181 atacagaggc tgatttctgg aaatctcaac tttctcaagt tgagaccgcc cggatatcta
      241 cctttcatag cttttgtctg ggacttattc gtgagcatcc gcttgagatc ggtatccccc
      301 cgatggtggg gatcttgggc gaaggggaag caaccatcta tctgaaacag tccattgaga
      361 cagtgttggc cgaggtcttt cgtggacaaa accaggataa ttcggaatta ttccggatcc
      421 tgctcgaatt cggctgggat tcctttgaga caagcctcgc cgggctttac gagaccatcc
      481 gggagagcgg aatccctttt gatgaagcgg aaaaacggag ccgtattgaa ctaaaggcag
      541 cgatagccgc aattcccgaa agtgtcacgg aattgtccga ggccgtatcg gaacttctgg
      601 agtatagccg ggacgcggcc ctaaccgaac gggccatgga actaatggcc gcattgcggg
      661 agagttggcc cgccctccgc gaggcaatca ccaacggagc cgtgctcgat gagactctac
      721 cggccttatc cgagcttagc gaggcgttac ctcgcaacca acccaactcc atcaaggaaa
      781 gagtcggtgc catccgcgac atggcagagg gattaagccg gaagttatta aatcaagaag
      841 ccctatgccg gctgccggcg ttcagggaaa ttttggagcg ggttgatgcg gcataccgcc
      901 ggttgaaaca agaccttggt tgtctggact ttaccgacca attgatcctg gcccgggatc
      961 tgctccggaa tcacccggat ttggcggagg aaatacgtgg cagtctgtgc tacttactgg
     1021 tagatgagtt ccaggatacc aacggtctac aaatggaact ggtccagctg ttatggggcg
     1081 agacgcatcc cggcggccgc tttatggcgg tgggtgatat caaacaatcg atctaccgtt
     1141 ttcgcggggc tgaagccgat ctcatcttga aacttgccga tgagctccgg gaggctactc
     1201 aaggacgttt aatctccttg agtagaaatt accgctccag cggtcgggtg attcaattcg
     1261 tcaatgccct gtcgatgccg ctctttatgg gggaaccctt tacctatgag ccgctgcttg
     1321 cggatgaacc gcaagggacg gctgcggtag agtttttatt gaccggagat ttcgatcggc
     1381 gaattgaagc gaaacttgtg gggaagcaga ttcaacaatt ggtctcgtca gtgccggatc
     1441 gggaaacggc ctggcattac ggagatattg tgctgctgtt tcggaccaaa accgccatcc
     1501 cgctttacca aaaggtattc caggaaatgg gaatccccta ttactcggcc agcagcagtg
     1561 ggttttatcg gtgccttgag gtgacggatc aattgaacct gctccgggtg acgctacagc
     1621 gttacgatgg agtggcgctt atggccctct taacctcggc ctatgtcggc ctcaatgatg
     1681 agtgcctttt ttggctgggt accgatgagg gcgcacacgc gcgcgatctg gtaggccggt
     1741 tttacgctgc cgacttcccg gcggaactgc ccaccctcgc ccggtataag ctgggacaat
     1801 tccgggaatt actgatttat cttcaggaac atcgtgattt gctgggaatt cccgggattt
     1861 tgcgggccgc cctggaacga acccattacc gggagatgct tcagatcatg cccaattcca
     1921 ttcagcgcca ggccaacctg gacaagctgt tggccaaggc cgacgagttc gtggccaagg
     1981 gcttccacga cctgcaccgt tttttagact acctcgagaa actggaaacc gcggaagtga
     2041 ttgagggaga ggcgtcgacc caggccgagg cgggcgatgt ggtccggtta atgacgatcc
     2101 atcgcgccaa gggacttgaa tttccggtag tgatcatccc cgatctcgac cggcagttct
     2161 cacgaggact ccgtggcaga ctggcttttc acaaaaacac cggaatcggt ttcactatcc
     2221 gttacggtga cggggagaac ggcgctccgt ctcattggga acaactaaag gcactcaacc
     2281 gcagggaaga gatagccgaa ttgaaacgct tgctttacgt ggctttgacc cgggctaaac
     2341 aattgctcat cttggccggt tcgggctgca actcttccaa ggcaaaaacc atagaaaccg
     2401 ctgataactg gatgaaatgg tttcaattac tgctgccttt ggaaggggca ggcgccgaac
     2461 tggactggga gggtgttccg ctccgaatca tccgggatct gccggcgtct gaggtaccac
     2521 ctaaagcgca gttattgatt gaggcccatc gcgccgaact atcgacggga attcccgggg
     2581 ctaccgccca agcagaaatg gagatcgcca gtacagcact ggcggggcag gaacggtcct
     2641 tgacattgaa ggtgaccgag gtgttaacct tcaagagctg cagcagacgt tatttttgga
     2701 agtaccggat gcgcttcccg gaagatccgg ctccgataga ggggtgggat tcggaagtga
     2761 aaggtccatc atctccggca gtggctgaac aggcggtaac gcttgaggct caaggaatcg
     2821 tgtcacctgt ggcgaccggt tccgcagaac gaagttacgg atcagcttat gttcttcgtt
     2881 ccggtgaatc tgtccggggt ctcggaacgc gaatcggaac ttttgtccat caagcgatcc
     2941 gcggcgtagc ctcggaatgg ccggaaacgc tttggagtga agtgttttat gatctgccgg
     3001 ggccggataa agaacagtta aagagggatt tgcagcaaat ctggcggaac ttccggaata
     3061 gtccatttac tgaacaagcg ggacaatttt gggatgaagt acctttccga atcaagctcg
     3121 gagagaagtt gaaaatggag gggcggtttg accggctgct gatgagtccg gacgggaaac
     3181 taattttggt tgattataag acgcatcgta gcagcgccgc caaggttccg gaactggcca
     3241 agggatatct ctggcaacta cagctgtatg ccatggccgt gaaggctttg tgggggcgtt
     3301 tacccgaccg ggcggtgctg tatttcatct attccaatca gacattttcg gtgcctttga
     3361 caccggaggc tctccggggg atggagagcg agatccggga gatcggccag ttcattgctg
     3421 tccatgatcg ggctgcggat tatccgtccg cagatgactg caaaggctgt ggttattcct
     3481 ggtgctgtaa actgtgatgt tccactgcgg cttgccctga ggaacaatcc tccatgtggg
     3541 tggcaaaggc gttatggtta tggatggatt cgtaactctc cacctccacc cgaaaccagt
     3601 tgattcgagg ctcggaacgt aaggccagga ccgaatccct gagaatatcc tcgacaaact
     3661 tcgggttctg ataggcggat tcggtgacga acttctcatc ctctcttttc aaaacaggat
     3721 acaccggaca actgccttga attcgcagta tggagatgag atcctcgagc cagaggatct
     3781 ttcccggata gccgaaacgg acaagggccc ggatggccgc ccgttggtta tgggcgccga
     3841 aggctgagat ctccttactg caggggcata atgacaacac gggcacctct acgcctaatt
     3901 ggtagtcgaa acagtgtcct tgttcaataa ggttgccgcg aaactccccg atataatcga
     3961 gagaacccac ggctgagctt accggtgcct tcagcggcaa aaagtagcga aacttcaaac
     4021 tgaactcagc ctccgccgcg ttgagatgat ggcacaattc agcgaggatc tggcggatct
     4081 ccttaccgga aatgggcttt tcactccagg aggtcaaaag ctccatgaaa cggctgagat
     4141 gagttccttt aaactcctgc ggcaggttta cggaggcgct gacattgccc tggacgtgtt
     4201 gaaatccacc gtccttcatt cggatctgaa gcgggagatg tacatcctta atcccgaccc
     4261 gttggatctc gattcctcgt tcatcccgca tattctgcac atcgatcatc gaaccgccgc
     4321 cttttttttt tagagtcgaa aatcgaaagt caatgaaggc atgaggcacg aggcaagagt
     4381 gttgtaagtc atctactctt tgtctaaagt ctaaagtccc aagtgccgta agtctattgg
     4441 caactcaccc ccgacccctc tcttttcgga aaggggtgta gggttatgct aaaatgcaaa
     4501 tagaaatacg cggggtgagt tactcacgcc tcatgcctca cgcctcatgc ctcatgcctc
     4561 atgcctattc cctattgcct atgatcttct gatagcaaac aatatccaga aaacggccga
     4621 attttttgcc aacctcttta tacagggcgc attgtacata acccaggcc
//
LOCUS       DASVMB010000139         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_606666,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000139 DASVMB010000000
VERSION     DASVMB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606666"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF531_04340"
     CDS             <1..232
                     /locus_tag="VF531_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713229.1"
                     /translation="KKKEPPPANFCKAVVNANFQSTMKQTQYKLVKYKLFALTQLKTT
                     SHESITAQKIPVATEGKPYALRGPNDGGTNCE"
     gene            complement(453..914)
                     /locus_tag="VF531_04345"
     CDS             complement(453..914)
                     /locus_tag="VF531_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713230.1"
                     /translation="MMITWAGLFLMICAAEVIGWLSRKRLGSLRLPFKRCFYNLISYH
                     LIGGVLTLILGSIALLYPWLDREFSYFCFISALIFLGEIFWIRQGLYEGGVVTADGSW
                     AWSKIAAYRLDDHWLQLQLKHKPLASLARMEVELGEGDRSRFEPFLVRHLS"
     gene            1159..2358
                     /locus_tag="VF531_04350"
     CDS             1159..2358
                     /locus_tag="VF531_04350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7713231.1"
                     /translation="MERRIMHIDMDAFFASVEQLDNPSYRGKPVIVGADPHGRGVVST
                     CSYEARRFGIHSAMPIREAFRRCPDGIFLPVRGRRYAEISRTIMNLLAEYTPLVEPLS
                     LDEAFLDLTGSEKIFGPALTIAREIVIRIQKEMGLPASVGLAPNKFLAKLASDLRKPR
                     GFVEITWDNLTEILDPLPISKLWGVGPKTEEQLRQIGIGTIGTLRRIDPKILEGRFGP
                     SGVQLYHLANGQDERPVETDETAKSIGHEITFQTDTDDRNFLAGILLWLADQVARRLR
                     RNGLKGRVINLKLRDTNFKTLTKRVTLATATDFEETIYCEALKLAQEVQWGAKRVRLL
                     GVSVSGFIQDEPQQLSLFETGKTTEPAELTRLHTAIDELRNRFGETIVTKGTILHVKE
                     RMDSKDE"
     gene            2461..>2714
                     /locus_tag="VF531_04355"
     CDS             2461..>2714
                     /locus_tag="VF531_04355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7713232.1"
                     /translation="MRKISGIWLCGWLLLGMSFGSQLVLYASAADNSWRIRINIPEYR
                     LELFNGIEHFKSYPVAIGRSETPSPMGNFFITVKVVNPTW"
BASE COUNT          736 a          629 c          703 g          646 t
ORIGIN      
        1 aaaaaaaaaa gaaccacctc cggccaattt ttgcaaggcc gtggttaatg ccaatttcca
       61 gtcaaccatg aaacaaacac aatacaaatt ggtaaaatac aagttatttg ctttgaccca
      121 attgaaaaca acttcgcatg agtcgattac agcgcagaaa attccggttg cgactgaagg
      181 aaagccgtat gcacttaggg gaccaaacga tggaggaacg aattgcgaat aatgattcgg
      241 ttcccgggag gctgttccct gcccggattt gaaaaaactg ttgtcaactc ctgggacccg
      301 agccatttta aatccaaggg gtcaaggttt aaaaaccgtc catgaagatg ggctcccggg
      361 tctcaaggga atggaagcat gaaagatcgg tcccgcttgt aactacggat gttactcacc
      421 ttaatgcgag tgaagataga tcagccagcc gtttatgaaa gatgccggac gaggaacggc
      481 tcaaagcgac tccggtcacc ctcgccgagt tcaacttcca ttcgagccag agaggcaagg
      541 ggtttgtgct ttaactgcag ttgcaaccaa tgatcgtcca aacggtaagc ggcgatcttc
      601 gaccaagccc aggaaccatc agccgtgaca actccgcctt catataaacc ctgtcttatc
      661 caaaaaattt ctcccagaaa aatgagcgcg ctgatgaaac agaaatagga aaattcacgg
      721 tcgagccaag gatatagcag ggcgatggat cccaagatga gggtgagtac cccgccgatc
      781 aaatgataac tgatgaggtt gtagaagcac cgtttgaaag gcagccgcaa agaacccaac
      841 cgtttgcggc tcaaccagcc gataacttcc gcggcgcata tcatcaagaa caacccggcc
      901 caagtaatca tcatcgtcac ccatttcttt tatgatcttg gttttcttaa gtttattcaa
      961 aagtttattc aaagacaacc ggttttcttc atgttccttc ctaacctgga acattcaggg
     1021 tatattggct aaacatacaa taccatatcg cccttgattt ggtcaaggtg aagaatgttc
     1081 gttgatactc aaaatgattt gaccaatcgc tctttccgtg aaataattga atggagagga
     1141 ttgaacgagg ggattcccat ggaacgccgg attatgcata tcgatatgga tgccttcttt
     1201 gcatccgtgg aacagttgga taacccaagt taccggggca aaccggtaat tgtgggagcg
     1261 gatccgcacg gccgtggggt ggtttcgact tgttcctacg aagcacgccg ttttggaata
     1321 cactcggcta tgccaatccg ggaggctttc aggcgttgcc cggacggtat ttttttacct
     1381 gtacggggcc ggcgttatgc ggagatctcc cggacaatca tgaatctact cgccgaatac
     1441 actcccctgg tcgaaccgct ttcgctcgat gaagcttttc tcgacttgac cggctcggaa
     1501 aagatcttcg gtcccgcctt aaccattgcc cgggagatcg tgatccggat ccagaaggag
     1561 atggggctcc cggcttcggt aggtctcgcc cccaacaaat ttctggccaa gctggcttcg
     1621 gatcttcgca aaccccgtgg tttcgtggag ataacttggg ataatcttac ggagatcctc
     1681 gatccgttgc caatctccaa actgtgggga gtaggcccta aaaccgagga gcaattgaga
     1741 cagatcggga tcgggactat cggcaccctg agaaggattg acccgaagat ccttgaggga
     1801 aggttcggcc cgtccggggt acagctttac catctggcca acggacagga tgagagaccg
     1861 gtggagacgg acgagaccgc caagagcatc ggacatgaaa ttacttttca gacggatacc
     1921 gatgatcgga actttttggc cggaattttg ttatggctcg cggatcaggt ggcccggagg
     1981 ttaaggcgaa atggccttaa aggccgagtg atcaatttga agctgcgcga taccaatttt
     2041 aagaccttga ccaaacgggt gactttggcc actgccaccg attttgaaga gaccatctac
     2101 tgcgaggcct taaaacttgc ccaggaggtt caatggggtg cgaaaagggt acgattactt
     2161 ggtgtcagtg tcagcggctt tattcaggat gaaccgcagc agttgagtct ttttgaaacc
     2221 gggaaaacca cggaacccgc ggagttaacc aggcttcaca cagccatcga tgagttgcgg
     2281 aatcgcttcg gggagacgat tgtgaccaaa gggaccattc tccacgtgaa agagcggatg
     2341 gacagtaagg atgaatgacg attggattat aaaaaaataa tttgaattat gcatggatgc
     2401 atagcccccg gttcatgacg gaatcctaca taggattatg gaaatgtgcg ggggaaatgg
     2461 atgcggaaaa ttagtggtat ttggctttgc ggttggttgc tgttgggcat gtcttttggc
     2521 tctcaactgg tgttgtacgc tagtgcggct gataattcgt ggcggattcg gatcaatata
     2581 cccgaatacc ggctggaatt attcaacggt atcgagcatt tcaagagtta tccggtagct
     2641 atcggcagat cggaaactcc atcaccgatg ggaaactttt tcatcaccgt taaggtagtc
     2701 aatcccacat ggta
//
LOCUS       DASVMB010000140         7174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_606783,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000140 DASVMB010000000
VERSION     DASVMB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606783"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..2572)
                     /locus_tag="VF531_04360"
     CDS             complement(218..2572)
                     /locus_tag="VF531_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HEX7713233.1"
                     /translation="MAELLGGNGVFLVHGKTIVENHPGVQPDEINAVLTGEGLEPLTE
                     ADLTPKIACTRTIAHQILVKHNTSQDWHRLQLRFDALASHDITYVGIIQTARASGLET
                     FPVPYVLTNCHNSLCAVGGTINEDDHLFGLSAAKKYGGIFVPAHQAVIHQYMREMMTG
                     CGKMILGSDSHTRYGALGTMGIGEGGPELVKQLLQRTYDISYPEVIAVYLEGQPNTGV
                     GPQDVALTLIQAVFKNGFVKNKVLEFVGPGVGHLSVDFRNGIDVMTTETTCLSSIWRT
                     DDRVHEYFRIHGRPEAYLRLEPGEVAYYDGLIRLDLSRIEPMIALPFHPSNVFSIREL
                     NQNPHEILAAVEEEGRRQLDNAKLAFHLADKIQDGRLRFDQGVVAGCAGGTFENLVAM
                     AEILDQKSVGNDAFGLSAYPASQPIQLELVRNGMLTKLMAAGVVIRSAFCGPCFGAGD
                     VPANGGLSARHTTRNFPNREGSKPGNGQIASVALMDARSIAATALNGGLLTPATDLGK
                     PVLKVAYAFNHQVYNNRVYQGFGKAQSQSELQFGPNIADWPKMQPLPENLLLKVAAVI
                     HDPVTTTDELIPSGETSSYRSNPYKLAEFTLSRKDPEYVGRAKAIQELEKERQRLLHD
                     GTAREPQSTELGALFLPLVPEANPTPERIHQLLETTGIGSIIFAVKPGDGSAREQAAS
                     CQKVLGGWGNIAEEYATKRYRSNLINWGMLPFTVEPGSLNLNVDDLLYLPGVRRAVAD
                     GATEIEAEIISGHGKTKIRLILANLSADERDIILAGCLINYYTK"
     gene            2999..3373
                     /locus_tag="VF531_04365"
     CDS             2999..3373
                     /locus_tag="VF531_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713234.1"
                     /translation="MPAKKEINLNMRSQPDFWFRSIENFKNVASVLLVLAVILLGLAA
                     PAVKGLLNQVLNLNLDTTWNRFLLTIDFYLFGLTFLVGLVGTFINLTRNRRRNDRLSL
                     ILIGASVISLVYLAVYLIVQGL"
     gene            complement(3458..4141)
                     /locus_tag="VF531_04370"
     CDS             complement(3458..4141)
                     /locus_tag="VF531_04370"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7713235.1"
                     /translation="MSDLGYRLKRFLYQEFIPVTKGVALGSGLVTLVLFILARLNLDF
                     SPWVALFPGEFPRTPWTLLTYPLFDQSLFSFLFALLWLWFVGGSLERTWGGRRYALLL
                     LAVTLITGLVMSLVGWFVGGMVPIYGLWLPLVGLTWAWAETDPYREVLFWGIIPLKAR
                     WLAWLDAILIFTMYLPVHFLMGAASLSGILAFYLFRGRGSAGGYGGGSRNRWNSQNRF
                     RRGRLRVIK"
     gene            4617..5009
                     /locus_tag="VF531_04375"
     CDS             4617..5009
                     /locus_tag="VF531_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713236.1"
                     /translation="MWNGWPLDRVLLLFTALAFLLIFIQVWLFHYRQNFRHWAMWIPV
                     LATPVLGVCLIILALGNTTRFRPVVVALLVAGLLGGLVGFYYHFRGVGTRVDGYRIQN
                     FLVGPPVILPLMVTAISVLGLMAIYWRS"
     gene            5006..5602
                     /locus_tag="VF531_04380"
     CDS             5006..5602
                     /locus_tag="VF531_04380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX7713237.1"
                     /translation="MMEERRTRYPGFNVLTEVKAWDPHTRQIVMRRLGPFPELQFFQT
                     DEAAVFKTIAGHLTYDYREEILTYVVSWADHRLSSKTGESQRKPGIPHESELIRNGLR
                     SLDYLGTGLYRREFFKLDPEQQFSILAALQTGSAPAATAGNLPQQEFFQKLLDLVIRP
                     YYSHPTIWSEIGYGGPAYPRGYVRVEIGLIDPWEAQHE"
     gene            5595..>7174
                     /locus_tag="VF531_04385"
     CDS             5595..>7174
                     /locus_tag="VF531_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7713238.1"
                     /translation="MSEQLPNLIHHCDHYADHRYADLNHRKYPKDADICIVGAGAAGG
                     VLAHDLSQAGLRVVVIEAGPFWDPQTDFASDELAMESLAWQDTRLSTGNDPLNFGHNN
                     SGRGVGGGTVHFTGVFLRFHGSDFRVRTRDGVAEDWPLAYEDLEPYYSRIEREIAVSG
                     PKEFPWGEFHGPYPYPAREPISANSEIFRRGCERLGIRSTVAPLAILSAPFDGRPPCI
                     NRGFCNQGCMPNAKFSTLIQHIPKAIQAGAEVLSDCMVTHIEMNPLGKVAGVHFVHDG
                     QEFYQTARLFILAAFCIETPRLLLNSACPQFPNGLANTSGLVGKFLTTHSGHDVLAKF
                     DEEIRLYKGTPVLATSQEFYEADPGRGFVRGYTLHGHGARPLGIAANLASKAGIWGKD
                     LYHIMRDYNYYAQVTMVGEVLPVESNCVTLGTEKDEYGLYRAQVTFSYGENDNRLIAH
                     GVRKAGEILEAAGGKAGIVIPDSAHLMGGSRMGSDPRTSVVDSFGRSHDISNLYICGA
                     SVFVTSGGANPTETVMALA"
BASE COUNT         1746 a         1906 c         1771 g         1751 t
ORIGIN      
        1 ttgccatgct tccgtttcat gacctgaaga gtaaaaaatc gtaaaatggt cggtctttaa
       61 catctgccat tccggccggg cgagggcgac cacaggaacc aaccataacc cgatcaggca
      121 aagacacaga atgcggatga gcttcatgag ttcctcctca aaataaaaag atccgcccag
      181 tcgcggatcg gatcttcccg atttcccatc ggtatgatta ttttgtgtaa taattaatca
      241 aacaccccgc cagaatgata tcccgttcat ccgctgagag attggccagt atcaaacgga
      301 ttttggtctt tccgtgtccg ctgatgattt cggcctcgat ttcggtagcg ccatccgcta
      361 ctgcccggcg caccccgggg agatacaaca agtcatcaac attcaagttg agcgaccctg
      421 gttccaccgt gaaggggagc attccccagt tgattaaatt actacggtac cgtttggtgg
      481 catactcctc ggcaatattt ccccaaccgc ccaaaacttt ctggcaggaa gccgcctgtt
      541 cacgggcgga accgtcaccc ggcttcaccg caaagataat actgccgatg ccggtcgttt
      601 ccaaaagctg atggatccgc tccggggtgg gattagcttc gggaaccagg ggtaaaaaca
      661 aagcgcccaa ttcggtcgat tgaggttccc tggcggtacc atcgtgcaaa agccgttgcc
      721 gctctttttc cagttcctgt atggctttgg cccggccgac atattcagga tctttccggg
      781 agagagtgaa ttctgccaat ttataaggat tggaacgata agatgaagtc tcccccgaag
      841 gtatcaactc atcggtagta gtaaccggat cgtgaatcac agccgccact ttcaggagca
      901 gattctccgg tagcggttgc atcttgggcc aatccgcgat gttcggcccg aattgcagct
      961 ccgattggga ttgtgccttg ccaaatcctt ggtaaacacg gttattataa acctggtgat
     1021 tgaaagcgta agccacctta agcaccggtt tgcccaggtc ggtggcaggg gtcaacaatc
     1081 ctccatttaa ggcggtagcc gcaatagaac gtgcatccat caaagccacc gaagcgattt
     1141 ggccgttccc gggtttggaa ccctcccggt tcgggaagtt ccgggtggta tgtctggcgc
     1201 tcaacccgcc gttggccggg acatcgcccg ccccgaaaca cggaccgcaa aatgccgaac
     1261 gaatcacaac cccggcggcc attaacttgg tcagcattcc gttccggacc aactcaagct
     1321 gaatcggctg gctggccgga taagcgctca aaccgaaggc atcattgcca acggattttt
     1381 ggtcgagaat ctccgccatc gctaccaaat tttcaaaggt accaccggca catcctgcta
     1441 caaccccttg gtcaaagcgg agccggccgt cttgaatctt atcggctaga tgaaacgcaa
     1501 gcttggcgtt gtccagctgt cttctgcctt cttcttcgac agccgccaag atctcgtggg
     1561 gattttgatt tagctcgcga atggaaaaaa cgttactggg atgaaacggt aacgcaatca
     1621 tcggctcgat ccggctcaaa tccaaacgga tcaatccatc gtaatatgca acttctccgg
     1681 gttccaggcg cagatacgcc tcaggacggc catgaatccg gaaatactca tgaacccgat
     1741 catcggttcg ccaaatggaa ctgaggcagg tagtttcggt ggtcattaca tcgatcccgt
     1801 tccggaaatc aacagaaaga tggccgacac cgggtcctac aaactccagg accttgtttt
     1861 taacaaaacc gtttttaaaa accgcctgaa taagcgtcaa agctacatcc tgaggcccga
     1921 cacccgtgtt cggctggcct tccaaatata ctgcaatcac ttcgggatag gagatatcgt
     1981 aggttctctg caataattgt ttgacaagct ccggtccgcc ctcgccgatc cccatcgttc
     2041 ccagggcgcc atagcgggta tggctgtccg agcccaaaat catcttgccg caaccagtca
     2101 tcatctcccg catatactga tgaatcacag cctgatgcgc ggggacaaat atccccccgt
     2161 actttttagc tgcggaaaga ccgaaaagat ggtcatcctc attgatggtc ccacccacgg
     2221 cacacaaact attatgacag ttggtcaaga cgtacggtac gggaaatgtc tcaagtccgc
     2281 tggcccttgc ggtttgaata attccgacat aggtgatatc gtgggaagcc aaggcatcga
     2341 aacgcagttg caatcgatgc caatcctggg acgtattatg tttcaccaat atctgatggg
     2401 cgatggttcg ggtacacgca attttcggtg tcaaatccgc ctcggtcagc ggttccaaac
     2461 cctcacccgt caaaaccgca ttaatctcat ccggctgcac ccccgggtga ttctctacaa
     2521 tcgttttgcc gtgaaccagg aaaactccgt ttcctcccaa taattccgcc accactatcc
     2581 cccgctttac attgcgttat acgcttattg cgttacacgc ttattacgtt atacgtattg
     2641 cgccacgcac tgattactgt aactgcgtat ctaaatttac tgcatatttt tggttgcata
     2701 aaataaacga ctcttttaca gttccttttt aaaacattaa aacattcttg attggccctt
     2761 gtcaagatat tcgtgacgat tccggaagat tccggccgtt atcggatttc gtcgcatttc
     2821 tcctgttaac ctgctttctc agaaaagatc ctgtttatgc aaataatttc gatgatgatt
     2881 tcctttgatt gtttccgaag gtaaaaagga ttttttcaga gcagtgtaga attaaattaa
     2941 agttgtacta agtatttggc ccgagacacc cctactctgg gaaaggcagg agattacaat
     3001 gcccgctaag aaagagatta acctgaatat gcgaagccaa cctgattttt ggtttcgatc
     3061 gattgagaac tttaaaaacg tggcaagcgt tctgcttgtg ttagccgtta tattgctggg
     3121 acttgcggcg ccggcggtta aaggattgtt gaatcaagta ctcaacctca acctggatac
     3181 cacatggaat cgttttttgc taaccatcga tttttacctt tttggcttga cttttcttgt
     3241 gggattggtc ggtacattca ttaacttaac ccgcaaccgg cggcgtaacg accggcttag
     3301 tctcatcttg atcggagcct cggttatttc tctggtgtac ctggccgtat atttaatcgt
     3361 tcaaggctta taaaccattc cactccggtg cttatttaca ataagaattt ataaagggtt
     3421 agggctcttc caacgagtcc taaccctttt gtttggttta tttgatgacc cgtaatctgc
     3481 cgcgtcgaaa tctattttga gaattccacc ggttccggga accgcccccg tatccaccgg
     3541 ctgacccacg gccgcgaaag agataaaacg ccaaaatgcc actgagtgat gccgctccca
     3601 tcaagaaatg aaccggcaga tacatggtga aaatgagaat ggcatccagc catgccagcc
     3661 accgggcttt gagcggaatt attccccaaa acaaaacctc ccggtagggg tcggtctcag
     3721 cccaagccca ggtcagtccg accaatggca accaaagacc atagatggga accatccctc
     3781 ccacaaacca gccgaccaga ctcataacga ggccggtgat taaggttaca gccaaaagta
     3841 acaatgcgta gcgccggcca ccccaggtcc gttcaagact acctccgacg aaccacagcc
     3901 atagtaaggc aaagagaaag gaaaacaagc tctgatcgaa caatggatag gttaataaag
     3961 tccagggggt gcgcggaaac tccccgggaa ataaggcgac ccaagggctg aaatcaagat
     4021 tcaaccgggc caatataaaa aggaccagcg taacaagacc gcttccaagg gctaccccct
     4081 tagtgaccgg aataaactct tggtacagaa accgtttcaa acgataaccg agatcgctca
     4141 atcttctctc ctcccaaccg gagtctctaa tagttatctt taccacattc ccgcctctca
     4201 gtcaatactg gagtacgaac tgcaaatcga gaggcaggtt cctgtctatt tcgttttaat
     4261 tcacaacgaa acataccaaa cacacgaaaa cgggtatgct tttatttgta gccattgaga
     4321 taatctatac gctgcggaga atggtagtaa ttctattata cactacaggg gctggtggtt
     4381 atctcctctt atcgcgtctg aaggacatcc gcctgataaa aaatttcgtg gatttagtga
     4441 ttttcgtggt taaaaatatc tctatcagta tgacaattta attcacaacg gaacacacca
     4501 aaccacggag aatggtggtt atctcctccc cgcgccgggg aggagacata agaggagggg
     4561 tttccggcca tattaataag gattttgtac cgtaaacaga gggaggagtt ttaaccttgt
     4621 ggaacggatg gccgcttgac cgggtcttgc tgctctttac cgccttggcc ttcttattga
     4681 tcttcattca agtctggcta tttcattacc gtcaaaactt ccgccattgg gccatgtgga
     4741 tcccggtact ggcgacaccg gtgctgggag tatgcctgat tattctggca ctgggcaaca
     4801 ccacccggtt tcgtccggtg gtggtagcgc ttctggtagc cggtctgctg ggtggtctgg
     4861 tgggttttta ttatcatttc cggggcgtcg gcacccgggt ggatggttac cggatccaaa
     4921 acttcctggt cggcccgccg gtaatcctgc ccttgatggt cacggccatt agcgtactgg
     4981 gcttgatggc catctactgg aggtcatgat ggaagaacgc cgtacccgct atccggggtt
     5041 taatgtctta acagaagtga aagcatggga ccctcatacc cgccagatcg tcatgaggcg
     5101 gctgggtccc ttccccgaac tgcaattctt tcaaacagat gaagcggccg tctttaaaac
     5161 catcgccggc catctcacct atgactaccg cgaggagatc ttaacctatg tcgtaagttg
     5221 ggccgatcac agactctcct cgaagaccgg cgagagtcag cgtaaaccgg gtattcctca
     5281 cgaatcggaa ttgatccgca acggattacg tagccttgac tatctgggaa ccggacttta
     5341 tcgacgggaa ttcttcaagc tcgatccgga gcagcaattc agcatcctgg cagctcttca
     5401 aactggtagc gcaccggcgg cgacagccgg taatctgcca caacaggagt tttttcagaa
     5461 gcttctcgat ttggtgatcc gtccctacta ctcccaccca acgatttggt cggagatcgg
     5521 gtacggcgga cccgcttacc cccgaggcta tgtccgggtt gaaattggct taatcgatcc
     5581 ttgggaggca caacatgagt gagcaactcc cgaacctgat ccatcattgc gaccattacg
     5641 ccgatcaccg ctatgctgat ctgaatcacc ggaaataccc caaagacgcc gatatttgta
     5701 tcgtcggtgc tggtgcggcc ggtggcgtat tagctcatga ccttagccag gccgggttgc
     5761 gggtagtggt gattgaagcg ggaccattct gggatcccca gaccgatttt gccagtgatg
     5821 agttggccat ggaatctctc gcttggcagg atacccggct gagtaccggg aatgaccctc
     5881 tcaatttcgg gcataacaat tccggacggg gagttggcgg tggaaccgtc catttcaccg
     5941 gggttttttt acgctttcat ggcagcgact tccgggtgcg gacccgggac ggggtagccg
     6001 aagattggcc gctagcctat gaagacctcg aaccttacta ttcccggatt gagcgggaga
     6061 tcgccgtctc cgggccgaaa gaatttccct ggggtgaatt tcacggaccc tatccttatc
     6121 cggcgcggga accgatcagc gctaattcgg agatcttccg ccgcggttgt gaacggttgg
     6181 ggatccggag tactgtagca ccgctcgcca tactctccgc cccctttgac ggccgtcccc
     6241 catgtattaa ccgggggttc tgcaatcaag gctgtatgcc caatgccaaa ttcagtacgc
     6301 ttatccagca tatccccaaa gccatccagg ccggggccga agttttaagt gactgcatgg
     6361 tgacgcatat cgaaatgaac ccgctgggaa aagtagccgg agtccacttt gtccatgacg
     6421 ggcaggaatt ttatcagaca gcccgcctct ttattctggc tgctttctgt attgagactc
     6481 ccagactact tttgaattct gcctgcccgc aatttcccaa tgggctagct aacacaagcg
     6541 gtctggtggg aaagttttta acgacccata gcggtcacga cgtattggct aaatttgacg
     6601 aggagatccg tctttataaa ggcacccctg tactcgcaac ctcgcaggag ttctatgagg
     6661 ccgatcccgg acggggcttt gtcagaggct acaccctgca tggtcatggc gcgcgtcccc
     6721 tggggattgc ggcaaacttg gcctccaagg cggggatctg gggtaaggat ctctaccaca
     6781 tcatgcggga ctataattat tatgctcaag tgacgatggt cggggaggtc ttgccggttg
     6841 agagcaactg tgtgacgctc ggtacagaaa aggacgaata cggcctatac cgggcgcagg
     6901 tgactttcag ttacggggag aacgataacc ggttaatcgc ccacggggtt cggaaagccg
     6961 gtgagatact cgaagccgcc ggaggtaagg ccggtatcgt catacccgat tcggcccatc
     7021 tgatgggcgg tagccgtatg ggctcggatc cccgcacctc agtagtagac tcattcggcc
     7081 gtagtcatga catttccaac ctttatatct gcggtgcgag tgtgtttgtt acttccggag
     7141 gagccaatcc cacggaaacc gtaatggcgc tggc
//
LOCUS       DASVMB010000141         6733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_164666,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000141 DASVMB010000000
VERSION     DASVMB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164666"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF531_04390"
     CDS             <1..255
                     /locus_tag="VF531_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7713239.1"
                     /translation="SIARAIRLGYLGDDLSQYAQKGYQGIIERLKFDEKGGLIIGDIC
                     IGTGIGDYAHYIARPRSENDLHGVGAFILMCVEMNQVRGI"
     gene            257..1648
                     /locus_tag="VF531_04395"
     CDS             257..1648
                     /locus_tag="VF531_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEX7713240.1"
                     /translation="MQYLNDQGRGVKIAYIGGGSRGWAWRLMADLALEPALSGEVFLY
                     DLDFEAAHDNEIIGNQLSVRDDVKGKWKYRTVKILEEALNGADFVVISIQPGTFKEMT
                     SDVHLPEKYGIYQSVGDTVGPGGLVRALRTIPIYQDFAIKIKKYAPDAWIINYTNPMS
                     LCTRTLYEVFPEIKAFGCCHEVFGTQKLLASMLKEELGIAGIPRDEIRINVLGINHFT
                     WLDQASFRGTDLFPLYRKFVEQYYESGFEGEGEDTWRTNNFKSAQRVKFDLFKRYGLI
                     AAAGDRHLAEFLPPTWYLKDPETVESWKFRLTPVDYRIGHQEQLELQRKRMIRGEEQL
                     PLKVSGEEGVRQIKALLGMGDLITNVNLPNHGQMADLPLKAVVETNALFSRDRIRPVV
                     AGALPPEINHLVVRHVWNQETILKAALSKNKETAFRAFINDPLVTIKPGDAEILFKEM
                     LANTCKYLPGWKL"
     gene            complement(1676..2836)
                     /locus_tag="VF531_04400"
     CDS             complement(1676..2836)
                     /locus_tag="VF531_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HEX7713241.1"
                     /translation="MPENSKKDRDNRVPAWFINGTLGIFLMVLGIVGVLFFKGERSGL
                     LCSNIPLLARSRLINLDTKTSRFILQNGIPFLERSVGEDDPFTLFSFNWTQIYWRLAA
                     NLKDPNPQEIIKTQFPLLALVAPEPPARLIKDVTLAQPPDQTEPEPVVSSSGVETTPT
                     NTAPVATLQPAVLIYHTHTSESYLPASGKDHLYPRGDIFQVGSYLQQVLENRYSIPCI
                     HAEEIHDQIPFRESYQRSQLTLKKYLKEYPNLQVVLDVHRDATPGVKASCVINGKQTA
                     TIGIVVGSDKMGLPHPNWRKNHQFAQKLAEAMNLYYPGLNSRVLITDARYNQHLHDHA
                     LLIEFGDQHSTLEEVNRAADLFASVLALVLHEEAEAAKTGSDKTQAPPMTTK"
     gene            3820..5244
                     /locus_tag="VF531_04405"
     CDS             3820..5244
                     /locus_tag="VF531_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7713242.1"
                     /translation="MEQMILLAILVPILGAFLLPIIGMYAPKVRNAMALVFIAASFMA
                     SAALVSPVLAGQSPTFVLPLPLGLDLIFTADALGVFMALAASFVGMIIILYSYGYIQH
                     DSNQNEYYMMVVVFLGSMVGLVYSQNLIYLYLFWEIAAICCWRLIGFYREREVILRAD
                     KAFLITVFGALAMLLGFIILYNDFGTFDLRAMHGQIAPNLAVLLIMFGIFSKSATLPF
                     HTWLPDAGVAPSPVTALLHAAVLVKIGVFAFARIFVGSIGMEPIWNQILPVVIAASAL
                     IAGGAALIETDIKRVIAYSTISQLAFIFLGLMMRNSLAIAGGLLFILMHGISKGGLFL
                     CAGIVEHNAHTKDIRKMGGLIKTMPVTAISFALCALSVMGIPPFGGFFPKYMVISGAV
                     VGGNPWIAGVFIIGAFLTVLYLLRLFTLVFLGEPKMPQVKEGTATMLWAVAILAGLSL
                     IGGFLVHFPNDFIQIALRQMMGSL"
     gene            5244..>6733
                     /locus_tag="VF531_04410"
     CDS             5244..>6733
                     /locus_tag="VF531_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7713243.1"
                     /translation="MEETLLWMLILIPAILGAVILAVPTQTRYLRGVIGSIASAFLVY
                     LALKLWGQELSITVPWAEFGIDFQLRLYPFSQFIVTAIAGFTFLVMLYSIRFMADRPK
                     NNQFYGFFFISEAMAIGAALANNLVVMLFFWEGLLITLFALISLGNERAYQTALKTFV
                     IVGLSDLCLMLGIGIVGAQANTLVMSEIHLDTVGLNGTAFVLMMIGAMAKAGAMPFHT
                     WIPDAAIDAPLPFMALLPAALEKLLGIYLVARIALDFFTLTMGMRILVMSIGAITIIL
                     AVMMALVQKDFKRLLSYHAISQVGYMILGIGTGIPIGVVGGLFHMINHAMYKSCLFLT
                     GGSVEKQAGTTDLHRLGGLWKVQPLTTICFVIAAASISGVPPFNGFVSKEMIFEGTLL
                     LGAGYRIFFAAAVLGALFTAASFLKLGHAVFFGKPDPQAKPTTEAHWTMTVPMVILAA
                     GCIFFGIWFKFPLEFLIKPALAVSLGPLVNNVHFAFGFGELFWLTVL"
BASE COUNT         1543 a         1522 c         1760 g         1908 t
ORIGIN      
        1 tcaatcgcca gggccatccg actgggctat ttgggggacg atctgagtca atatgcccaa
       61 aaaggatatc aaggaattat tgaacggctc aagtttgatg aaaaaggcgg attgattatc
      121 ggagacatct gcatcggcac gggtatcggg gactacgctc attatatcgc caggcccaga
      181 agcgaaaacg acctgcatgg cgtaggcgca ttcattttga tgtgcgttga gatgaatcaa
      241 gtgaggggga tctaaagtgc aatatcttaa cgatcagggc agaggggtta aaattgcata
      301 tatcggtgga ggctcccggg gatgggcctg gagactaatg gccgacctgg ctttggaacc
      361 agccctctcg ggagaggtgt ttttatacga tcttgatttt gaagcggcac atgataatga
      421 gattatcggt aatcaattgt ctgtccgaga cgatgttaaa ggaaaatgga agtaccgcac
      481 ggtaaaaata ctggaggagg ccttgaacgg cgcggatttc gtagtgatct cgatccaacc
      541 gggtactttc aaggaaatga cctcggatgt ccatttgccg gaaaagtacg gcatttatca
      601 gtcggtaggg gatacggtcg gtcccggcgg cctggtgcgt gctttgcgga ccatcccgat
      661 ttatcaggat ttcgcaatta aaatcaaaaa atatgctccg gatgcctgga taattaatta
      721 taccaatcca atgtcccttt gtacccgaac cctctatgag gtatttccgg aaataaaagc
      781 ttttggttgt tgccatgagg tttttggaac tcagaagtta ttagcgtcaa tgctcaagga
      841 ggagttaggt atcgcaggaa taccccggga tgagatccga atcaatgtcc tgggaatcaa
      901 ccattttacc tggttagacc aggcctcttt ccggggaact gatcttttcc cgttataccg
      961 gaagtttgtg gagcagtact atgagagcgg gttcgaaggt gaaggcgaag atacttggag
     1021 gactaataac tttaaatcgg cccagcgggt taagttcgat ttgtttaaaa gatacgggtt
     1081 gattgctgcc gccggagatc gtcatttggc ggaattcctg ccgccgacat ggtatttaaa
     1141 ggatccggaa accgttgaat cttggaaatt ccggcttact ccggtcgact accgcatcgg
     1201 acaccaggaa caattggaat tgcaacgaaa gcgaatgatc cggggcgagg aacagcttcc
     1261 tttgaaagtc tccggagaag agggggtccg tcaaatcaaa gcccttttag ggatgggcga
     1321 tttaataacc aacgttaatc tccccaacca tggtcagatg gcggatcttc ccttaaaagc
     1381 cgtggtcgag accaatgcct tgtttagcag ggaccgaatc cggccggtgg tggccggggc
     1441 gttaccgccg gagattaatc atttagtggt taggcatgtc tggaatcaag aaactatcct
     1501 taaggcagct ctgtctaaga ataaggagac ggcttttcgg gcttttatca atgacccgct
     1561 ggtaacaatt aagccgggcg atgccgaaat attattcaaa gaaatgttgg caaatacttg
     1621 taaatacctc cccggatgga aattgtgacg accgctcatt ttaaaagcat ccctcttatt
     1681 tggtggtcat cggaggagcc tgcgtcttat cggaaccggt ctttgctgcc tccgcctctt
     1741 catgcaagac taatgccaat acgcttgcga ataagtccgc tgcccgatta acctcttcca
     1801 gagtagaatg ttgatcgcca aattcaataa gcaatgcatg atcatgcaga tgttgattgt
     1861 aacgggcatc cgtaatcaaa acgcggctgt ttagacccgg ataatataga ttcatcgcct
     1921 cggcaagttt ctgcgcgaac tggtgattct tgcgccagtt ggggtgggga agccccatct
     1981 tgtcgcttcc caccacaatc ccgatggtgg cggtttgttt gccgttgatt acacaactgg
     2041 ctttaacccc cggggtggca tcccggtgaa catcgagaac cacttggagg ttggggtatt
     2101 ccttcagata ttttttaagt gtgagctggg agcgctggta tgattcgcga aaggggatct
     2161 gatcatggat ctcttccgcg tgaatacagg gaatggagta acggttctcc aaaacctgtt
     2221 gtaaataact ccccacctgg aagatgtcgc cccgggggta aagatgatcc ttaccactcg
     2281 ccggaaggta agattcggag gtgtgggtgt gatagatcaa gaccgccggt tgaagggtag
     2341 cgacgggagc cgtattcgtc ggcgttgtct ccaccccgga ggaactgacc acgggttccg
     2401 gttcggtctg atccggcggt tgagccagcg tcacatcttt tatcaatcgg gcggggggtt
     2461 ccggtgcaac cagagccaag agcggaaatt gggttttgat gatctcttgc ggattcgggt
     2521 cttttaagtt ggcagccagc cgccaataaa tctgagtcca attgaagctg aataacgtga
     2581 agggatcgtc ctctccgaca gaacgttcca aaaaagggat gccattttgc aggataaacc
     2641 ggctggtctt ggtatcgagg ttgatcaatc gggacctggc aaggagtgga atattcgaac
     2701 agagcaaccc cgaacgttcc cccttaaaga aaagtactcc cacaatgccc aggaccataa
     2761 ggaagatacc gagggtaccg ttgataaacc aggctggaac tctattatcc ctgtctttct
     2821 tggaattttc cggcatctag tccacctttc tttgcctaaa atcatatgcg cggacaaatt
     2881 gtttttagaa ccggaatgta aggctataac caccaagcgt tcgtcggcag tagattggta
     2941 aaattctgtc ggaacgtttc ggtaactaag ttgtaaaatc gaacatatac tgatcgggtg
     3001 ttgcataaga ttctccttgt ctgagcatcc ggcgccagat aaggggctgg aattaaatat
     3061 actctggagg taatgctatg ttttgtttgg actgcggcgc taaggttgaa tcgtggcggg
     3121 aatattgcgg acgttgtgga gcagatcttt ttaggcggga gcattcgaaa acttccgtaa
     3181 accacacgct ttttacccgg ttttttcgaa atccccgtta acagcctggc ggccaatcca
     3241 gccgccaggc ttttttttgg tcaataggga acaggcatga ggcgtgaggc aagaggcaat
     3301 agggaacagg caacaggcaa ggagcggggc gtatgatgtg ggcctaccta gtttaggtcg
     3361 gaagcctatc gacctgagat tccggccggt tccctgaaaa aggtgacacc gttttcttaa
     3421 gagttgtgat tacaactcca ctttaatggg gatgggtaga tgggcgtcgt gccatgattt
     3481 ttttgcttac gacttccgaa tattaatttc gaattgtaaa tgatctcctt ggaaaaattt
     3541 accctttagc aggaaaaata gtttttgggc agggaatccg gttgaatttg ccgaaatata
     3601 caaagtcatg gctgtttagg tgattcatgg cacaggaaaa ataccttgac agaggggaaa
     3661 agaagagtaa aataaatttg ggttttgttt gtataattcc cggaatccgt ttgaattcat
     3721 ggctttggtg tttttttcaa ttcattttaa ctattttaca tacagtaaca tttttaactt
     3781 ttattccaat ttgatcttaa ttccaagtga gggaaaacaa tggaacaaat gattctgctt
     3841 gcgattttag tccccatatt gggagcattt ttattgccca tcatcggtat gtatgcgcca
     3901 aaagtccgga acgccatggc tttagtcttt attgcagcct cctttatggc atccgccgcc
     3961 cttgtgtctc cggttttggc cggtcagtca cccacgttcg tcctaccgtt acctctaggg
     4021 cttgacctga tttttacagc ggatgcgctt ggagtattca tggcactcgc cgcatcattc
     4081 gtcggaatga tcattatttt gtattcttac ggctatattc aacatgattc gaatcagaat
     4141 gagtattaca tgatggttgt ggttttttta ggatccatgg tcggcctggt ttactcacaa
     4201 aaccttatct atctgtatct cttctgggaa atcgccgcta tctgctgctg gaggttgatc
     4261 gggttttacc gggagcggga ggtcatcctc cgggctgaca aagcgttcct gatcactgtt
     4321 ttcggcgctt tggccatgtt actcggcttc attattctct ataatgattt cggcactttc
     4381 gatctccggg ccatgcatgg tcagatcgct ccgaatctgg cagtattgtt gattatgttc
     4441 ggaatcttct ccaaatcggc cacgttgccg tttcacactt ggttgccgga tgcgggtgtt
     4501 gctccctcac cggtgactgc gctactccac gccgctgttc tggttaagat cggagttttt
     4561 gcctttgccc ggatctttgt gggcagtatt ggaatggaac cgatttggaa ccagattctc
     4621 ccagttgtga ttgcagccag cgccctgatt gccggcggcg ccgctttgat tgaaaccgat
     4681 atcaagaggg tcatcgccta ttcgaccatc agccaattag cctttatctt cctgggcctg
     4741 atgatgcgca actccttggc gatagccggc ggcttacttt tcattctgat gcacgggata
     4801 tccaaaggcg gccttttctt gtgcgccggg atcgtagaac acaatgccca taccaaagac
     4861 attcggaaaa tgggcggctt aatcaaaaca atgccggtca cggccatatc tttcgccctt
     4921 tgtgcgcttt cggtaatggg aattccgcca ttcggcggtt tctttcctaa atatatggta
     4981 atctccggag cggtcgtcgg cggtaacccg tggatcgccg gagtctttat catcggcgct
     5041 tttcttactg tgctgtatct gttacgcctc tttaccttgg tctttttggg agagcccaaa
     5101 atgccccagg ttaaggaagg caccgccacg atgctttggg cagtggcaat acttgccgga
     5161 ctgtctctaa tagggggatt tctggttcac tttccgaatg atttcattca aatcgcctta
     5221 cggcagatga tggggagtct ataatggaag aaacgttatt atggatgttg attttgatcc
     5281 cggcgatact tggtgccgtt atattggcgg ttccgaccca gacccgttat ctccggggag
     5341 ttatcggctc gatcgcctca gccttcctgg tttacctggc attgaagctt tgggggcagg
     5401 agctgagtat caccgttcct tgggccgagt ttggaatcga ttttcagttg cgattgtatc
     5461 cttttagtca gtttattgtt accgctattg ccggttttac ttttttggtg atgctctact
     5521 cgatccgctt tatggccgat cggcctaaaa acaaccagtt ctatggtttc ttctttatct
     5581 ccgaggcgat ggccattgga gccgccttgg ccaataacct ggtggtaatg ctttttttct
     5641 gggaaggact tttaatcacg ctttttgcgc tcatctcttt gggaaacgag cgggcgtacc
     5701 aaaccgcact taaaaccttt gtgattgtgg gtctctccga tctctgcctg atgttgggga
     5761 ttggcatcgt cggcgcccag gccaataccc tggttatgtc ggagattcat ctggataccg
     5821 taggtttaaa cggaacggcc tttgtattaa tgatgatcgg agcaatggcc aaggccggtg
     5881 ccatgccttt tcacacctgg atccccgatg ccgccattga tgcgccgcta ccgtttatgg
     5941 cgttacttcc ggcggccctg gaaaaactgc ttggcatcta tctggtggcc cgaatcgctt
     6001 tagacttttt tactcttacg atggggatgc ggattttggt catgtccatc ggagccataa
     6061 cgattattct cgccgtgatg atggccttgg ttcagaagga tttcaaacgc cttctttctt
     6121 accacgctat cagtcaggtg ggttatatga tcctgggtat cgggaccgga atccccattg
     6181 gtgtggtggg cggtcttttt catatgatca accacgctat gtataagagt tgtctcttct
     6241 taaccggcgg ttcggtggag aaacaagccg gaactactga tctacatcgt ttgggtggtt
     6301 tatggaaggt tcaaccgctg actacaatct gttttgtgat tgccgctgct tccatctccg
     6361 gggtaccgcc tttcaacggg tttgtttcca aggagatgat ctttgaaggg acgctgctcc
     6421 tcggcgccgg ataccggatt ttttttgctg ctgccgtgtt gggtgcgtta tttaccgccg
     6481 catcattcct gaaactgggg catgccgttt tctttggcaa gccggacccg caggcgaaac
     6541 cgaccaccga ggctcattgg accatgacgg tacctatggt gattttggcg gccggttgta
     6601 ttttcttcgg aatctggttc aaattccccc tggaattttt gatcaaaccg gctctggcag
     6661 tgagcctcgg tccgttagta aataacgtac atttcgcctt cggctttgga gagcttttct
     6721 ggttgactgt gct
//
LOCUS       DASVMB010000142        13818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_311924,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000142 DASVMB010000000
VERSION     DASVMB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311924"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VF531_04415"
     CDS             <1..929
                     /locus_tag="VF531_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7713244.1"
                     /translation="LNYDEKISVSEETREAIFRAAAELNYKKKTINPKIENVALLYWP
                     KNQEEFEDVYYKSIRQELEKQSPERNIRLIRYNKGEGIQTVSKTSKAFIAIGWFDMDE
                     INCLKKITRHGIFIDTSPDESCFDSVRPNLDSIVTQIVDYFVEQGHKNIGFIGGTDFN
                     ILTGKPSMDVREWSFRESAKYYNRLNEQNLFITDTFSVSEGYRLGIKAIEELGENMPT
                     AFCIASDTLAIGVLQAFNEKGWDIPKRVAFFSINNISIAQYLSPPLTTFHIDIPLMCD
                     AALNLLQERMLKGRNITKTVYINGRAIFRKSC"
     gene            complement(1066..1992)
                     /locus_tag="VF531_04420"
     CDS             complement(1066..1992)
                     /locus_tag="VF531_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX7713245.1"
                     /translation="MLPINAVDKELLAEIADLHAVPSGAYNIRKNGTAIARESTPGIE
                     IIPKSDKPGIDIRISPGVVNQSVHIPVIITEGGLNDLVYNTFEVGEGAEVLIVAGCGI
                     HNDSSSKSQHDGIHEFFIRKGAKIRYIEKHYGEGGGTGERVLNPVTIIHLEEGASAEL
                     ELVQVRGIHHTVRKTEAQVGDKARLLMNERLLTHESQQATSEVTVDLQGIGSSGEVLS
                     RSVARDQSRQIFQVRLIGETQCKGHVACDSIIMDQADIRSQPELWAKSAAAELTHEAA
                     IGRLAGDQILKLMTFGMSEAEAVQTLLQGYLR"
     gene            complement(1970..2725)
                     /locus_tag="VF531_04425"
     CDS             complement(1970..2725)
                     /locus_tag="VF531_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7713246.1"
                     /translation="MLKLEEIDLKLGDGPEAAEILQGITLGFETGKLYGITGPNGGGK
                     TSVAKVIMGIHQPKRGRLWFDGKEITALSIAERARLGIRYAFQNPPRFKGIDIAWFLH
                     LAGPNANEQQIRSIMRQIGLCPEDYLKRMADTGLSGGEMKRLEIASVLLGETRVAVLD
                     EPEAGVDLWGFEQLLDLVIRSHRRSPDRTTIIISHSQRFLEAADEIIVMAGGRVQERG
                     SMERIRPMLEEGIRCQWRKSCLEGDADVTNQCS"
     gene            complement(2864..2951)
                     /locus_tag="VF531_04430"
     tRNA            complement(2864..2951)
                     /locus_tag="VF531_04430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2915..2917),aa:Ser,seq:gga)
     gene            complement(3030..3119)
                     /locus_tag="VF531_04435"
     tRNA            complement(3030..3119)
                     /locus_tag="VF531_04435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3083..3085),aa:Ser,seq:cga)
     gene            complement(3165..3638)
                     /gene="tadA"
                     /locus_tag="VF531_04440"
     CDS             complement(3165..3638)
                     /gene="tadA"
                     /locus_tag="VF531_04440"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX7713247.1"
                     /translation="MTANSDDVYIREALIEASKAAELGEVPIGAIIVYQGEIIARAHN
                     LRESTADPTAHAELLALREAARVRQHWRLNTAVLYTTLEPCPMCAGAMVLARIARVVY
                     GAPDPKAGAAGTLMNIVQDSRLNHRLEVTAGVLEKDCALILKDFFRERRKTFDNC"
     regulatory      4112..4204
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4268..5269
                     /locus_tag="VF531_04445"
     CDS             4268..5269
                     /locus_tag="VF531_04445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7713248.1"
                     /translation="MYQYLGIRKTYGLRISLRRIRTVAIVFIFIFCAWRLSPQNSLSW
                     MVNLLLTAAILILEYRRDRRTLNRSGAGARDILIMLGVNLLICLNVAISGGVTGPLKF
                     LLLVPVLFYTEEFGIGIGAINILPLIGFWLASIQVYFKAPFNAGLDQWVFLLCAIITW
                     LIMGTNHRISLRYEQKVGRLLIKDDLTGLYNRRFLKANILGAIRIKHPFALVIMDINY
                     FKYYNDNWGHLQGDVLLIYVGRLLKETVPNTGMVVRYSGDEFVAFLPRAGQKEVDQFC
                     DGVTQRLQQNHFPGEECFPNGTISLAYGSVFYPDQVTHYEGLVAAADEMLYRNKKHR"
     gene            complement(5275..5535)
                     /locus_tag="VF531_04450"
     CDS             complement(5275..5535)
                     /locus_tag="VF531_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713249.1"
                     /translation="MREIVGEPVQVEALFQQGRIKPGYFAWQRRLYKVKGITGHWSVA
                     DGFFRNHHFTVLTGEPNLYELAFSTQSLRWELVCIYHESCEF"
     gene            complement(5637..7565)
                     /gene="gyrB"
                     /locus_tag="VF531_04455"
     CDS             complement(5637..7565)
                     /gene="gyrB"
                     /locus_tag="VF531_04455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX7713250.1"
                     /translation="MDSEKEIEKENGSTDYNASQIQVLEGLEAVRRRPGMYIGSTCVK
                     GLHHLVYEIVDNSIDEVINGFCDLINVSINADGSVTVEDNGRGIPVDIHPKEKKSALE
                     VVMTILHAGGKFDNEGYKVSGGLHGVGASVVNALSEETEVWVRSKGNVYYQRYHKGKP
                     DEPVKIIGETDGTGTTTRFKPDPEIFETCEFSFDFLTHRLREMAFLNRGVKIILKDEK
                     SGKETVFHYTGGIISFVEHLNKTKDPIHKEIIYICKKVDTNEIEVAIQYNDSYVETIF
                     SFANNINTYEGGTHLSGFRSALTRTLNDYARKINLIKENEASLSGEDVREGLTAVINV
                     KLRNPQFEGQTKTKLGNSEMRGIVETSLNEGLAEFFEENPLVVKKILEKCLLAAKARE
                     AARKAKELTRRKSALEGSSLPGKLADCSFREPELSELYLVEGDSAGGSAKQGRDRRFQ
                     AILPLRGKILNVEKASLDKVLANEEIKAMITAMGTGFGEDFDLSKLRYHKIITMTDAD
                     VDGSHIRTLLLTFFYRFMTPLVEKGHVYIAQPPLYQVKQNKNEYYSYSDQELEELLKK
                     IGRQNITVQRYKGLGEMNAEQLWETTMNPEHRTILQVTLEDALEADQTFSMLMGDKVE
                     PRKEFIQSHASEVRNLDI"
     gene            complement(7634..7891)
                     /locus_tag="VF531_04460"
     CDS             complement(7634..7891)
                     /locus_tag="VF531_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018671244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HEX7713251.1"
                     /translation="MFIHLGGDTLISSKDIIAIISVENVSDADCRKVFLKGQDQKEIK
                     RIDSENCKSIIITDKKIYLSPISTLTLKKRAEFLNEIALKS"
     gene            complement(7937..8509)
                     /locus_tag="VF531_04465"
     CDS             complement(7937..8509)
                     /locus_tag="VF531_04465"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX7713252.1"
                     /translation="MSSSSFSGIIEAFFQRNGGWKRIKGELAVYYWPRIAGKGLASKV
                     EANRYRDGILYLVTENAALAHQITLMSPEIILRYRKVLGQGIIKSIKIKVGSTNIITV
                     PDQENPDVQLDRETETMISACGEEIQDPQLAEKFVHFMQKSYMNQLVKEATGGRQCKK
                     CGIIIENGFDYCPCCERGNEGSQESAGAGE"
     gene            complement(8499..9641)
                     /gene="recF"
                     /locus_tag="VF531_04470"
     CDS             complement(8499..9641)
                     /gene="recF"
                     /locus_tag="VF531_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX7713253.1"
                     /translation="MFLNKLKAENFRNYSSLDLGFSPDINIFFGRNAQGKTNLLEAIS
                     LLALGRSFRTKKEEELIRWDTEHCYTVGEFLGSELDTRIEIGIGRGEKKVKIDGKPVR
                     RNELLGRVPIVFFGPDDLQLVKGGPAGRREFIDLYLAQIEPEYRLIYYNFYKVLQQRN
                     RLLKEIHPDRNELEAWNEQLIDRGTQVILYRMRLVEQIKPFINQAHHQISASTESLNI
                     EYLTFRRRLQRSATLSDVVEWENLMDREMIEGIFRSEIERMGRVEMERHITLVGPQLD
                     DLRLTLGSDIELRDFGSQGQQRTASLALKLGLIAKIKENRGEFPLLLLDDVMSEFDDC
                     RKQALLQNMVGSVQTFMTSTSRRDFPIPTDQARFFEVEQGAVKNVQ"
     gene            complement(9916..11010)
                     /gene="dnaN"
                     /locus_tag="VF531_04475"
     CDS             complement(9916..11010)
                     /gene="dnaN"
                     /locus_tag="VF531_04475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7713254.1"
                     /translation="MKIVCTQDELSRFLQIVSRAIASKTTLPILTGILMETNENSLRC
                     LATDLEIAIEVTVPNIQVLEPGSIVLPGKTFVDIIKHLPPYPIEIEFNDRSKMVSINS
                     HHSSYQLPVLPLEEFPTLPELKDGEKVVINNGILKDGIRQTIYATLIEDPRPFLSSIL
                     WEINHDKLRLVATDVNRLAIRDMKIQSETEKTALVPVRSLREASNIFGTGDQDDLTVY
                     LTDKLIFIEGLGIRFSSRLVEAQFPRYEPVIPKEFKGSFTVKRNEMIEALERTVLVST
                     SLKININQTNLIITAQEPDKGHSKEEVEIDLSGEPMEIGFNGRFLLDFLKTVESERIG
                     FKYVEEQKPALLQGENETEYEYIVMPLKLA"
     gene            complement(11304..12623)
                     /gene="dnaA"
                     /locus_tag="VF531_04480"
     CDS             complement(11304..12623)
                     /gene="dnaA"
                     /locus_tag="VF531_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7713255.1"
                     /translation="MNNLESIWEKTLESISPHLSKPSFETWLKPTKAISLEGNIMVIE
                     VPNDFARDWLESRYAPLLSSTLKELLEDDVELKFVTHDHQEGEAQKVASVNTPLPQPF
                     QLNPKYTFESFVVGESNRFAHAASLAVAEAPGKAYNPLFIYGGVGLGKTHLMQAIGQF
                     ALKHNNDFRVVYASSEKFTNEMVNAIRFNRTPQFRDTYRNVDILLVDDIQFFAGKEGT
                     QEEFFHTFNSLYESGRQIVISSDRPPKEIPTLEDRLRSRFEWGLITDIQAPDLETRIA
                     ILRKKASVEGWHLPNDVIVNIADRINSNIRELEGALIRIIAYASFHNKQISLDLANEV
                     LKDVISSTSSKRVTIPLIQQAVAEFFNIEIDDLKAQRRTKNITLPRQIAMYLVRELTD
                     YSLPKIGEEFGGRDHTTVIHAYEKIQDLIKTDPEVDRIIKNLIHKLS"
     gene            13325..13459
                     /gene="rpmH"
                     /locus_tag="VF531_04485"
     CDS             13325..13459
                     /gene="rpmH"
                     /locus_tag="VF531_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7713256.1"
                     /translation="MKMTYQPNRRKYKKDHGFLVRMATKNGRAVLNRRRAKGRKVLSA
                     "
BASE COUNT         3746 a         3259 c         2872 g         3941 t
ORIGIN      
        1 tattgaatta tgatgaaaaa atctcggtaa gcgaagaaac aagagaagca atattccggg
       61 ccgccgcaga attgaattat aaaaaaaaga caatcaaccc gaaaattgaa aatgttgccc
      121 tgctgtactg gccaaaaaac caggaggaat ttgaggatgt ttactacaag agtatcaggc
      181 aggaacttga aaagcagtct ccggagcgaa acatcagatt gatcaggtat aacaagggag
      241 agggtattca aaccgtaagt aaaacttcca aagctttcat tgcaatcggc tggtttgata
      301 tggatgaaat caattgttta aaaaaaataa cccggcacgg aatttttatt gacacttccc
      361 cggatgaaag ctgttttgac tctgtcagac ctaatctgga ttctattgtg acgcaaatcg
      421 tcgattactt cgtcgaacag gggcataaaa atataggttt tatcggcggc accgatttta
      481 atatattgac aggaaagcct tccatggatg taagagaatg gtctttcaga gaaagtgcca
      541 agtattacaa taggttaaat gagcaaaacc tattcatcac cgacacgttt tcagtcagcg
      601 agggttaccg gctcgggatt aaggcgatag aggagttagg agaaaatatg cctactgcat
      661 tttgtatagc cagcgacaca ctggctatcg gcgttctcca agcatttaac gaaaaaggtt
      721 gggacattcc aaagcgcgta gcttttttta gtatcaacaa tattagtatc gcgcagtatc
      781 tttccccgcc ccttactacc tttcatatcg atattccgct catgtgtgac gccgcgttga
      841 accttctgca ggagagaatg ctcaagggaa gaaatataac caaaacagta tacattaacg
      901 gaagggcaat cttcagaaaa agctgttgat atctctttcc ggcaccattt gtctacatgc
      961 aattgacaaa aaatccccca tacgggggaa gtattgaaaa agaacagtag gagattgatc
     1021 gcttaacgat ttcatttaac ttttcctgca ttaagatcga agactctatc ggagataccc
     1081 ttggagtaaa gtttgaaccg cttctgcttc gctcatacca aaggtcatca gcttaagaat
     1141 ctgatccccc gccaatctac cgatagccgc ctcatgagtc agttccgccg cagcgctctt
     1201 ggcccaaagt tccggttggg aacgaatgtc cgcttggtcc ataatgatcg agtcgcaagc
     1261 aacatgcccc ttgcactgag tctcaccgat aagacgaact tggaagattt gtcgcgattg
     1321 atccctggct acagagcgcg atagaacctc cccgctgctt ccaatccctt gaagatctac
     1381 cgtaacctcg gaggtagcct gttgggactc atgagtaagc aatctttcat tcatcaacag
     1441 ccgggctttg tcaccgacct gtgcctcggt tttgcgaacc gtatgatgga tccctctcac
     1501 ctgaaccagt tccaattccg cgctggctcc ttcctccaaa tggatgatgg ttacaggatt
     1561 tagcaccctt tctccggtcc cgccgccctc gccgtagtgt ttttcaatat agcggatctt
     1621 ggcccctttc cggataaaaa actcatgtat gccgtcatgc tggcttttac tgcttgaatc
     1681 gttatggata ccgcaccctg cgacgatcaa tacttcagcg ccttcaccca cttcaaaagt
     1741 gttatagacg agatcgttca atccgccttc cgtaataatc accgggatat gtaccgactg
     1801 attaaccact ccagggctga ttcggatgtc aatcccgggt ttgtcgcttt tagggatgat
     1861 ctcgattccc ggtgtggatt cccgggcgat ggcggttccg tttttccgga tattataagc
     1921 cccgctgggt actgcatgaa gatcagcaat ttcggccaat aactctttat caactgcatt
     1981 gattggtaac atccgcatcc ccctctaagc aagatttccg ccactgacac cggatacctt
     2041 cttcaagcat cggacggatt cgttccatgc tgccacgttc ttgcacccgg ccgccggcca
     2101 ttacgataat ctcatcggca gcctccaaga aacgttggct atgggagatg atgatggtgg
     2161 tccgatccgg agatctccgg tgggaacgaa taaccaggtc cagcagttgc tcaaaccccc
     2221 ataggtcaac gccggcctca ggttcgtcaa gcactgccac ccttgtttca cctaaaagta
     2281 ccgaagcgat ctcaagtcgt ttcatttctc ccccggagag cccggtatcg gccatccgtt
     2341 tgaggtaatc ttcggggcaa agaccgattt ggcgcatgat cgatcggatc tgttgttcat
     2401 tggcgttagg ccctgccaag tgtagaaacc aagcgatgtc gatccctttg aaacgcggcg
     2461 gattttgaaa agcatagcgg attcccaggc gggcgcgttc cgcgatggaa agagcggtga
     2521 tctctttccc gtcaaaccat aaccggccgc gttttggttg gtggattccc atgatcacct
     2581 ttgccactga ggtttttcct ccgccattcg gaccggtaat cccatataac ttacccgttt
     2641 caaagccgag ggtaatacct tgcagtatct ctgcagcctc cggaccatcc cctaacttca
     2701 ggtcgatctc ttctaatttc agcaaaggta atcgcgcccc tttcaaaaaa ttgatctact
     2761 tcgatgtatc cggaaccctt tggtgatttt ttattcatct ttggccaatt tttccagtaa
     2821 tacaaaaaag aggcggttat cccacctctg ttagaaacta taatggcgga gagataggga
     2881 ttcgaaccct agaggctgtt acacctacac gcgctccagg cgtgcgcctt caaccgctcg
     2941 gccatctctc cggttaaact aaagtgtaaa tttgagaaaa ttgtaaaacc tttttacctt
     3001 ttgcaatttt gacttttcaa ttgctcttat ggcggagaga gcgagattcg aactcgcgag
     3061 ggacgtaaat ccctacccgc tttcgaggcg ggcgccttca accgctcagc catctctcca
     3121 gatgggcgct ctcaaaccgt ccattcatta ttatctttta tcattcaaca attgtcaaat
     3181 gtttttcgcc gttccctaaa aaaatctttt aggataagcg cgcaatcctt ttccagtact
     3241 ccggcagtga cctccagacg gtggttcaac cgggaatcct gaacgatatt catcagggtt
     3301 ccggcggccc ctgccttggg atccggtgcc ccgtatacca ctcgggcgat acgggccaga
     3361 accatagcac cagcgcacat tggacaaggc tctaaagtgg tgtagagtac ggcagtgttc
     3421 aaacgccaat gttgtcgaac ccgggccgct tcccttaagg ccagcaactc cgcatgggcg
     3481 gtcggatcag cggtggactc ccgtaaatta tgggctcggg cgataatctc tccttgataa
     3541 acaatgatcg cgccgatagg gacttctcct aattcggccg ctttcgacgc ttcaatcaga
     3601 gcttcccgga tatagacatc atctgaattc gccgtcattt gaattcatac tctaagaacg
     3661 aatctgctac cgggcagaat agcatcaatt gcttcatgaa tcacacccac aatatattat
     3721 cacatctcta cgaaataatg caagctcgct ttattagaaa tcattcttgg attttccaac
     3781 atgggtttgg atatcgatca acctcaagca ggtactaatt gaaacattaa ggaaagttag
     3841 cgcgcttgcg cgcacactct cgtaaataaa cataaaacct tgttggtgac aaaaatatac
     3901 aagattctat aattataaaa gagattatga agcaattcaa gattgttccc aattgccatt
     3961 tggtgtataa ttcagataat aatcgcagat tcaatcagca acgcggttgg cgatctgttg
     4021 gacgcttcat ttagccaatg atactttaat tcattatttt cctttattta tctgtaagcc
     4081 actggagtac ctgagcgaac aacagcaact tatacgaaaa tggcaaattc ggcgaaagcc
     4141 gaagacgcaa agccacggat ctacagcgag ttcgctgcct cgccaagatc gccgggttac
     4201 cgaaatgagt tgtttttcta ttcccatctt tgtttagacc gtgactccac gttcacggag
     4261 gtgcgttatg taccaatatc tgggaattag aaaaacttac ggactaagaa tctccttgag
     4321 acggatccgg accgtagcaa ttgtattcat atttatattt tgtgcctgga ggctctcccc
     4381 tcaaaacagt ctgtcatgga tggttaattt actactcacc gctgcaatac tgatattgga
     4441 gtatcgccgt gaccgccgta ccctgaatcg atccggcgca ggagcccgcg atatactcat
     4501 tatgctcggg gtaaatctcc tcatctgcct taatgtggcc atcagtggtg gagtcaccgg
     4561 tcccctaaaa ttcctgcttt tagtgccggt actcttttat accgaagaat tcgggatcgg
     4621 gataggggcc atcaatattc tgcccttgat aggcttttgg ttggcaagta ttcaagtata
     4681 cttcaaggca ccattcaacg ccgggctcga tcaatgggtt tttctgctat gcgccatcat
     4741 cacctggttg atcatgggta ccaatcatcg tatttccctc cgttacgagc aaaaagtcgg
     4801 acgattgctg atcaaggatg accttaccgg attgtataac cgccgctttc ttaaggccaa
     4861 tatcttgggg gcgatccgta tcaaacatcc attcgctctc gttatcatgg atatcaacta
     4921 tttcaaatat tacaatgata attggggtca tcttcaaggg gatgtgcttt taatctatgt
     4981 cggccgactg ttgaaggaga ccgttcccaa tacgggaatg gtcgtccgat attcggggga
     5041 tgagtttgtc gctttcctgc cccgggcggg gcagaaggaa gttgaccaat tttgcgatgg
     5101 agtaacccag agattacaac aaaaccattt cccgggagaa gaatgttttc ccaatggtac
     5161 catatcactg gcctacggtt cggtattcta ccccgatcaa gtcacccatt atgaaggttt
     5221 agtcgctgcg gcagatgaga tgttgtaccg aaataagaag caccgctaga aaacttaaaa
     5281 ttcacagctt tcatggtaga tgcaaaccag ttcccaacgg aggctttggg tagaaaatgc
     5341 aagttcatat aggttaggct ccccagtcaa caccgtaaaa tggtggttgc ggaaaaatcc
     5401 atccgccacc gaccaatggc cggtaatccc tttaacctta taaagccgcc gctgccaagc
     5461 aaaataccca ggtttaatcc gtccctgttg aaaaagggct tccacctgaa ccggctcgcc
     5521 gacgatctcg cgcatattgg ttcacctcat ggcaaacatt tgttcgatat ccaatatatc
     5581 aggttaatat ttctttgtca agaggaagat ccccggcaag cggtggtaga aagctactaa
     5641 atatccaggt tccgcacctc tgaagcgtgg ctctggatga actcctttct gggctcaact
     5701 ttatccccca tcagcatgga aaaggtttga tccgcctcga gggcatcctc caaggtaact
     5761 tgaaggatcg tacggtgctc gggattcatg gtcgtctccc agagttgctc ggcattcatt
     5821 tcacccagac ctttataccg ttggaccgtg atattctgac ggccgatctt ttttaataac
     5881 tcctccaatt cctgatccga atatgaataa tactcatttt tgttctgctt gacctgatag
     5941 agtgggggct gggcgatata gacgtgtcct ttttcgacca gaggcgtcat aaaccgataa
     6001 aaaaaggtca gcagcagggt gcggatatgg gaaccgtcca catcggcatc ggtcattgtt
     6061 ataattttat gataacgtaa tttggagaga tcgaaatcct caccgaaacc ggttcccatc
     6121 gcagtaatca tcgctttgat ctcttcattt gccaatacct tgtcgaggct ggccttttct
     6181 acattgagga tcttgccgcg taaaggtaaa attgcctgaa aacgacggtc tcttccttgt
     6241 ttggccgatc caccggccga gtcgccctct accagataca actcggagag ctcaggctca
     6301 cggaaggaac aatccgccaa ttttcccggt agtgaactac cctccagggc actcttacgt
     6361 cgggtaagtt ccttggcttt tcgggcagcc tcacgggctt tcgctgctaa aagacacttt
     6421 tcaagaatct tcttgacaac cagcggattt tcttcaaaaa attccgccaa cccctcgttt
     6481 agcgatgttt caacgatccc ccgcatctca ctgttgccga gtttggtttt ggtttgcccc
     6541 tcaaactgcg gattgcgtaa cttaacatta attacagctg ttaaaccctc acggacgtct
     6601 tcgccgctga gcgatgcctc attttctttg ataagattga ttttacgcgc atagtcgttt
     6661 aaagtgcggg tcaatgccga tcgaaatcct gatagatggg tcccgccttc ataggtattg
     6721 atgttgttgg cgaaggaaaa aatcgtctcg acataactgt cattatactg aatggctacc
     6781 tcaatctcgt tggtgtcaac ttttttacaa atatagataa tctccttgtg aatcggatct
     6841 ttcgttttat ttaaatgctc gacaaaggag ataatccctc cggtgtaatg gaatactgtt
     6901 tctttgccgc ttttttcatc tttaagaatt atctttacac cgcggtttag aaaagccatc
     6961 tctctcaaac ggtgggtcag aaagtcaaaa ctgaattcac aagtttcgaa aatctccgga
     7021 tccggcttaa accgggtggt ggttccggtt ccatcagtct caccgatgat ttttaccggt
     7081 tcatcaggtt tacccttatg gtagcgttga taataaacat tccccttact acgcacccat
     7141 acttcggttt cttcagataa ggcattgacc accgaggcgc caacaccatg caaacccccg
     7201 gatactttat acccctcatt gtcgaactta ccgccggcgt ggagtatggt cattaccact
     7261 tccaaggcgg atttcttttc tttgggatga atatctacgg gtataccccg gccgttatcc
     7321 tcaacagtca ccgatccgtc ggcgtttatc gaaacattga tcagatcgca aaaaccgttg
     7381 atcacttcgt ctatactgtt atcaacgatc tcatatacta aatggtgcag tcctttgacg
     7441 caagtactgc cgatatacat gcccggtctg cgcctaaccg cttccaagcc ttccaacacc
     7501 tgaatttggc tggcattata atcagtggaa ccgttttcct tttcgatctc cttctccgaa
     7561 tccattctat aactccttcc caaatcataa gttgaaatta ctaggcttaa tctgttattt
     7621 ttaaattcga attttatgac ttcagggcta tttcattcaa gaattcagcc cttttcttta
     7681 aggtaagcgt ggagatcggc gacaggtaaa tctttttatc cgtgatgata attgatttac
     7741 agttctcgga gtcgatcctt tttatctcct tttgatcttg tcctttgaga aatactttac
     7801 ggcaatccgc atcggaaaca ttttcaacac ttataatggc aataatatct ttggagctta
     7861 ttaaagtatc tccgccaaga tgaataaaca ttccgaatcc tcccgtccta atcgactgta
     7921 aatcctcgac tctacctcat tcacccgctc ccgccgactc ttggctcccc tcgtttcctc
     7981 gctcgcagca agggcagtaa tcaaaaccat tttcaataat aatgccgcac tttttacatt
     8041 gtcggccgcc ggtggcttcc tttactaatt gattcatata tgatttctgc atgaaatgga
     8101 caaacttctc agccagttgc ggatcttgga tttcttcccc gcaagcggag atcatggtct
     8161 cggtttcccg gtcaagctgc acatcgggat tctcttgatc aggtaccgtg atgatattgg
     8221 tggaacctac cttgatttta atcgatttaa taattccctg ccccaacact ttacggtacc
     8281 tgaggataat ctccggactc atcagtgtta tctgatgggc taaagccgca ttttctgtaa
     8341 ccagatagag gataccatcc ctataacggt tggcttccac tttggatgcc agtccttttc
     8401 ctgcgatccg cggccaatag taaaccgcaa gttccccttt gattcgcttc caaccgccgt
     8461 ttctttgaaa aaaagcttca ataattccag agaacgaact actggacatt ctttaccgcc
     8521 ccttgttcca cctcaaaaaa gcgtgcctga tctgttggga taggaaaatc tcttctgctg
     8581 gttgaggtca taaacgtctg aaccgatcca accatgtttt ggagtaaagc ttgtttacgg
     8641 cagtcgtcaa attcagacat tacatcatcg agcagcaata aaggaaactc tccgcgattc
     8701 tctttgatct tggcgatcag tcctaatttg agcgccagcg atgcggtgcg ttgttgtcct
     8761 tgggatccaa aatcccgtag ttcaatatcc gacccaaggg tgagtcgaag atcgtccagt
     8821 tgcggaccga ccaaggttat atgacgttcc atttcaaccc ggcccatcct ttctatctcg
     8881 ctccgaaata tgccctcaat catctctcga tccatcaaat tctcccactc caccacatca
     8941 gataaagtag cggatctttg caagcgacga cggaaagtta aatattcaat atttaaactt
     9001 tccgtgctgg cgctaatttg atggtgggct tggttgatga atggcttgat ctgctcaaca
     9061 agacgcatcc ggtaaagaat cacctgggta ccgcgatcga tcaattgttc attccaagcc
     9121 tcaagttcgt tgcgatccgg gtggatctcc tttagtaagc gattgcgttg ttgcaacact
     9181 ttataaaaat tataatatat caaccggtat tccggctcga tctgcgcaag gtagagatcg
     9241 ataaattctc ttcggcctgc cggtccaccc ttcaccagtt gtaaatcgtc aggcccaaaa
     9301 aaaactatcg gcacccttcc taaaagctca ttccgacgta ccggcttacc gtcgatcttt
     9361 actttctttt caccgcgacc aatcccgatt tcaattcggg tatccagttc actgcctaaa
     9421 aattccccta ctgtataaca gtgttccgta tcccaacgaa tcaactcttc ctcttttttt
     9481 gtacgaaagg aacgtcccaa cgctaaaaga gaaatagcct ccaataaatt agttttacct
     9541 tgggcgtttc gcccaaaaaa gatattgata tcgggtgaaa acccaagatc taatgaggag
     9601 taatttctaa aattttcggc ttttaattta tttaaaaaca tagcgatacc tttcctatag
     9661 acccaagcta atttaattcg ctttttgatc ggtcctttcc tgcatcggta ttccttttga
     9721 taatataaaa actttattca atgatagtaa gcgggtttaa atttaaagcc gcaattatgc
     9781 ggcattaaat tttcgcgctc acccgcccct ttcttcataa aagaggaaac aagacaggtg
     9841 agtcgcaagg taaaggaata tccaattgaa aatgagtatg atgggagaaa gtttgtttat
     9901 cgattcattt tcagcttaag ctaattttaa cggcatcacg atgtactcgt attctgtttc
     9961 gttttcaccc tgaagcaaag cgggtttttg ttcttccaca tatttaaagc cgattcgttc
    10021 actctcaacc gttttcaaaa agtcgagcag aaaacgtccg ttaaacccaa tttccatagg
    10081 ttctcccgag agatcgattt ctacctcttc cttggaatgg cctttatcag gttcctgggc
    10141 ggtaatgatc aaattggttt gattgatatt aatctttaac gaagtgctga ccaaaacggt
    10201 tctttccagg gcttcgatca tctcgttccg cttcacagtg aaggatccct taaattcttt
    10261 tggaataacc ggctcgtacc gtggaaattg agcttccacc aaacgtgaag agaaccggat
    10321 acccaaaccc tcaataaaaa taagtttatc agttaaatat acggtgaggt catcctgatc
    10381 cccggtgcca aaaatattgg acgcttcgcg gagcgaacga accggaacca gggcagtttt
    10441 ctcggtttcg ctctgaatct tcatatcccg gattgccaac cggttcacgt cggttgcgac
    10501 cagacgtaat ttatcatgat ttatctccca caagatactt gataaaaacg gacgcggatc
    10561 ttcgattagt gtggcataaa tggtctgacg gatgccatct tttaaaatac cattgttaat
    10621 aacgactttt tcgccgtctt tcaactccgg aagagtgggg aattcttcca aaggcagcac
    10681 gggtaactga tatgatgaat gatgactatt gatcgatacc atttttgaac gatcattgaa
    10741 ttcaatctcg attggatacg gaggcaagtg tttgataata tccacaaaag tcttcccggg
    10801 aagtacgatc gaacccggct caagtacctg tatattaggt actgtgactt cgatggcgat
    10861 ctccagatcc gtagccaaac aacgcaaaga attttcattc gtttccatta aaataccggt
    10921 taaaatcggt aatgttgtct tggatgcgat cgctctagat acaatctgaa gaaatcgaga
    10981 aagctcatct tgggtacaaa cgatcttcat tacatactcc ccctttattc gatgtcaatg
    11041 gttcggttgt ttttttagaa tacgcccgga tggttataat tactattctt tttatatata
    11101 ttaaatataa aaatagtagc agtagtatta gtcacggttg aaatgtggat aagtccgata
    11161 aatgcagatg aggattgata tcaacacacc gtattttctg tggagaattc catcgtttat
    11221 tcagaaaatc accatattca caatatgcaa cctattgtgg aaaaaaaata atcacacgat
    11281 tcacaagtga atccacgaaa agatcaactt aacttgtgga ttaaattctt gatgattcga
    11341 tccacttccg gatcggtttt gattaaatct tgaatttttt cgtaagcatg gattactgtg
    11401 gtatgatcac gtccgccgaa ttcctcgcca atcttcggca atgagtaatc cgtaagttcg
    11461 cggactaaat acatggcgat ttgtcttggt aatgtaatat ttttcgtgcg acgctgcgcc
    11521 tttagatcgt caatttcaat attaaagaat tcggctactg cttgttgaat taatggtatc
    11581 gtcacccgtt tacttgatgt ggaagatatt acatctttta aaacctcatt cgccaggtcc
    11641 agactaattt gtttattatg aaaagatgca taagcgatga tccggatcag tgcaccctcc
    11701 agttcccgga tattggagtt gatccgatcc gcgatattga cgatcacgtc gttcggtaag
    11761 tgccatcctt caacacttgc ctttttacga agaattgcga tcctggtttc cagatcggga
    11821 gcttgaatgt cggtgatcaa accccactca aaacgggaac ggagtcgatc ttccaaagta
    11881 ggtatctctt ttggagggcg atcacttgag ataacgatct ggcgtcccga ttcatagagc
    11941 gaattaaagg tgtgaaagaa ctcttcctgc gtaccttctt taccggcgaa aaattgtata
    12001 tcgtcaacta ataatatatc gacgttacgg tatgtatcac gaaactgggg tgtacggtta
    12061 aatcgaatgg cattaaccat ttcattggtg aatttttccg aagaagcata aaccacacga
    12121 aaatcattgt tatgtttaag tgcaaattga ccgatggctt gcatcagatg agtcttgccg
    12181 agaccaacgc cgccgtatat aaagagcgga ttgtatgctt taccgggagc ttcggctaca
    12241 gccaaagagg cggcatgggc gaaacggtta ctttctccga caacaaaaga ttcaaaagtg
    12301 tattttggat tcagttgaaa gggctgtggc aagggagtat ttacagacgc taccttttgg
    12361 gcttcaccct cttggtgatc atgggtgaca aactttaact ccacatcgtc ctcaagcaat
    12421 tcctttaatg tagaggataa aagcggagca taacgcgatt ccaaccagtc tctggcaaag
    12481 tcatttggaa cttcaatcac cattatattg ccttccaaag atatcgcttt ggtaggttta
    12541 agccaggttt caaaactcgg tttgctcaaa tggggggaga ttgactccag cgtcttttcc
    12601 catatcgact ccaagttatt cattagaaaa atacactccc caagcttaat ctgtgaatta
    12661 gaaaaccaaa tagctctttt tatgaaaaga aatacgctga aaaaaatgaa agtaaagatc
    12721 tcatctaaac ctaatttatc cacaaccagt ggaaaagctg tttataaaac aggaatcaat
    12781 gaagtttgaa aggccaaaac cccaaaaaag ggaaaaaaaa taaagccccc gacaacaaca
    12841 tttttatata tccacagata tttagttatc cacaaaatta tcaacaacct gtggatggat
    12901 tttttcgaac gttactatca taacaaaaga tcttcccgtt ggcaatttct aattccccaa
    12961 aaaattggaa gggcgataac gatagagagc tctcctcgcc taattaagct gttttagtaa
    13021 taattaaaat tttggacttc aaaaccattc gatgcaaagg tctatattta gatgaatatt
    13081 ggttttgcca cgatatccac agtccggccg ggtttgttgt gcaaaatgtg aatattcata
    13141 gaaactattg acaccgcatg acttgattat tataattaaa tcgattattt ttgctttgtg
    13201 aattggcggc ttccgtttgt ggaaaactaa agtaaatttt ttatagccca agtttatccc
    13261 gttaacccgt aataagaatt attggcttta taaagtttaa aaaggcttaa ggtaggtgat
    13321 acccatgaaa atgacttatc agcccaatcg tcgtaaatac aaaaaggatc acggcttttt
    13381 ggttcgtatg gccacaaaga acggtcgtgc tgttctaaac cgtagaaggg cgaagggtcg
    13441 taaggtttta tcggcgtaat gatgaagcgc aagcctggga aaaggtcgat aacgttcgac
    13501 ccgaaagatg ctttacagac ttgcgcctca taaacgctcc atcgtggaac cgccgaaacc
    13561 ttgaatcaat tggaaacact caaaaaaaac tcagaattcc aattagtttt tgaaaatggc
    13621 cactcaattc acggaaagta tttggtggtc tattttcttc ataattcttt taactgtaac
    13681 cggtttggtt tctgtgtcgg gaagaaactt ggttcagcag tctgtcgtaa tcgggtcaaa
    13741 cgccggttac gtgaggcggt ccgtcagatt gtctttccta atgactccgc ctgggatatc
    13801 atttttgtgg ctcgctat
//
LOCUS       DASVMB010000143         3967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_311955,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000143 DASVMB010000000
VERSION     DASVMB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311955"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VF531_04490"
     CDS             <1..1161
                     /locus_tag="VF531_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7713257.1"
                     /translation="NQVGLVQEYGETKVAVETSFPAIYRIQPATAQHHLILDLEGATL
                     TPEIGSVPGDNRWIRSIVARQLNPTTVRLEIVFVKDIDCHVIRVPESPNRIEISATQR
                     LTGIKWEETGSGGKLTLTASGSIAARLYQTNNTPEELRLDLENLKPAPGVELPAVQNA
                     LITAIHWVDQHSVPAGIGVSVGQFVSYRPEFSTDRKTLVIQFHHSPLVGKTIVLDAGH
                     GGDDNGACGRQGTREKDNNLEIVMRLKDLLEAAGAKVVLTRTGDYFVSLYERSFLANF
                     VRADLFISVHTNNQPDLNARGMEVYRYRNRTAAQSLAEDILRRMGQTTGLKPVKVMQE
                     SFIVIRESEMPGVLVEVGYLSNFEEENIIRTPEFRADAARGIFQGIMDYYGG"
     gene            1316..1999
                     /locus_tag="VF531_04495"
     CDS             1316..1999
                     /locus_tag="VF531_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7713258.1"
                     /translation="MTGPLYDMLSSIPNSSLTEKVYQMLRQAILERKIQPGEKLNLDA
                     LAKTFNTSRTPVTYALKRLAFEGLVEIKERKKTSVINLTPEMIQETFDLRRVLEVYAA
                     EQAVKFVTGEEISSLRDLAAEMEREADYSKFLDQDRRFHENFIDIARNNKLTAIYRSL
                     HVHSQVMIECYLRPDKRIEATLIEHQRMIELLEKRELEPLIELIKDHIELVKGNLLKK
                     PDGSKADPE"
     gene            2067..2453
                     /locus_tag="VF531_04500"
     CDS             2067..2453
                     /locus_tag="VF531_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7713259.1"
                     /translation="MIQGIEHVGVLAKDTAALRDWYNRLFGFRTVYDNGKGTYFIMAP
                     DGSMLELIKEEEAAPAGGLKTGGLRHLALKVTDTDFEPLVAQLKKEQVVVVTDAAVSS
                     SGVKTFFFRDPEGNIVHLISRPKPLG"
     gene            2673..3317
                     /locus_tag="VF531_04505"
     CDS             2673..3317
                     /locus_tag="VF531_04505"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEX7713260.1"
                     /translation="MSKEAILARIKQSGLVAVVRAESSEQAAKITEACIEGGVAAIEI
                     TFTVPGAADVIGSLVRKYSASGKIIIGAGTVLDPETARTAILAGAQYVVSPCLNWDLV
                     KLCNRYQIACMPGAMTIKEVVECMEAGADVVKIFPGELFGPKIIKAIKGPIPYAPMMP
                     TGGVSLENVEEWIKAGAVAVGVGGSLIAGAKKGDYASITAIAKQFIEKIQAARA"
     gene            3329..>3967
                     /locus_tag="VF531_04510"
     CDS             3329..>3967
                     /locus_tag="VF531_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7713261.1"
                     /translation="MMASVVCFGEIMLRLSPPGYLRFVQTDSFDAVYGGGEANVSVSL
                     ANYGMDAAFVTKVPDNPIGQAAVNELRRYGVDAGFIARGGERLGIYFAEKGASQRPSK
                     VVYDRKYSSISQAKPEDFDWPKILKGARWFHFTGITPALGDNVAAIALAACKAARDLG
                     VTVSCDLNYRKNLWSTEKAGKVMGELMPYVNLCIANEEDAEKVFGIKAADTDV"
BASE COUNT          987 a          976 c         1146 g          858 t
ORIGIN      
        1 aatcaagtgg gtttggttca agaatatggg gagacaaagg tggctgtaga gaccagtttt
       61 ccggctatat accggattca accagccacg gcgcaacacc atctcattct tgacctggaa
      121 ggtgccactt tgacaccgga aatcggatcc gtaccgggag acaatcgctg gatccggagc
      181 attgtcgcgc gacaactgaa tccaaccact gtccggttgg agattgtttt cgtaaaggat
      241 atcgattgtc atgtcatccg ggttcccgaa agtcccaacc gaatcgagat tagcgcaacc
      301 caaaggctga ccggaataaa atgggaagag accggctccg gggggaagct gacattgaca
      361 gccagtgggt cgattgcggc ccgcctttat caaacgaata acactccgga ggagctgcgg
      421 ttggatctgg aaaatctcaa gcctgctccg ggggtagagc ttccagccgt tcaaaacgca
      481 ctgatcaccg ccattcattg ggttgaccag cattcagtgc ctgcagggat tggggtcagc
      541 gtaggacagt ttgtctccta ccgtcccgag ttttctacgg accgaaagac gctggtgata
      601 cagtttcatc attcgcccct tgtaggcaag accattgttc ttgatgccgg acatggcggt
      661 gatgacaatg gggcgtgcgg ccggcagggc acccgggaga aggataacaa tctggagatc
      721 gtcatgcgcc tcaaggatct gcttgaggcg gccggagcca aagtcgtgtt gacccggacc
      781 ggagattact ttgtcagcct gtatgagcga tcctttttgg ccaattttgt ccgggccgat
      841 ctattcatca gcgtacatac caacaaccaa ccagacttaa atgcacgcgg catggaggtt
      901 tatcgttacc gaaaccgcac cgcggcccaa agcctggctg aggatatctt gcgacgaatg
      961 ggtcagacta ccggattgaa acctgttaaa gtgatgcaag agagttttat cgtcattcgg
     1021 gagagtgaga tgcccggggt tctagtggaa gtgggatatc tctccaactt tgaagaggag
     1081 aatatcatcc gtaccccgga gttccgggcc gacgctgccc gtggcatttt ccagggaatt
     1141 atggattatt acgggggata aggggataga gaattaaatc ccggaaaaat tcttccattg
     1201 caggaataaa ttttgaagca gcgaatattt ttatcatctg tgaaatgtta cactttacac
     1261 tttacatttt atcctcacct tagccatgac aatgaaccgg taaaggatga ttcatatgac
     1321 gggacctctt tatgatatgt tgtcatccat tcccaatagc agcttgacgg agaaagtata
     1381 tcaaatgttg cgtcaggcca ttctggagcg aaagattcag cccggggaaa agctgaatct
     1441 ggatgccttg gccaagactt tcaataccag cagaaccccg gtgacctatg cccttaaaag
     1501 gttggccttt gaagggctgg tggagatcaa ggaacggaag aaaaccagcg tgatcaatct
     1561 tacgccggag atgattcaag aaacctttga ccttcgcagg gttttagagg tatatgccgc
     1621 cgaacaggca gtgaaatttg tgaccggaga agagatatcc tctctcaggg acttagctgc
     1681 tgaaatggaa cgggaggccg actactcgaa gtttctggat caggatcgcc gctttcatga
     1741 gaactttatt gatatcgccc ggaataataa acttactgcg atttaccgga gcttacatgt
     1801 tcactctcag gtcatgatcg aatgttacct ccgaccggat aaacggattg aggccacctt
     1861 gatcgagcac caaaggatga ttgaattgct ggaaaaaaga gaacttgagc ccctgatcga
     1921 gctgattaaa gaccatattg aactggttaa aggaaatctt ttaaaaaaac ccgacggctc
     1981 gaaggcggat ccggagtagc aaggcggttc catatcgtat atcggaaccg atttcataat
     2041 tcacgatgat ttcaggaggc gaaaaaatga ttcaaggcat cgaacatgta ggggtgctgg
     2101 ccaaagacac cgcagcctta agagactggt acaacagact tttcggcttc cggaccgtct
     2161 atgacaatgg taaggggaca tatttcatca tggccccgga cgggtccatg ctggaactga
     2221 tcaaagaaga ggaagcggct ccggccgggg gcttgaaaac cggcggcctg cgacatttgg
     2281 ctcttaaggt gacggacacc gatttcgaac cgctggtagc ccagttgaaa aaggagcagg
     2341 tcgtcgtagt caccgacgcg gctgtttcct caagcggagt aaagaccttc ttcttccgtg
     2401 atccggaagg gaatatcgtg catctgatct caaggcccaa acctctaggg taagggccgt
     2461 aaggcccggc cctaacccta gccccgagtg caaggtgata gttgggtttt aggcatgagg
     2521 cgactcaccc ccttagtcct cctctctccc gacaagcggg aaagagggga aaagctggtt
     2581 caagccagtg aaatagacgc gaagggggag agcaagagag gggttggggg ttgggctccg
     2641 aagactcata gaattagaaa ggatgaatca tcatgtcaaa agaagcaatt ttggcccgga
     2701 tcaaacaatc gggcctggtg gctgtggtcc gggccgaaag ctcggaacag gcggctaaaa
     2761 tcaccgaagc ctgtatcgag ggcggagtgg cggcgattga gatcaccttt accgttccgg
     2821 gagccgcgga tgtgattggg agtctggtcc ggaaatattc cgcctccgga aagatcatca
     2881 tcggcgccgg aaccgtgctc gatccggaga ccgcccgcac cgccattttg gccggggcgc
     2941 aatatgtggt cagtccctgc ctcaactggg atctggtcaa gctttgcaac cgttatcaga
     3001 tcgcctgcat gcccggggcg atgaccatca aggaagtcgt ggaatgcatg gaggccgggg
     3061 cggatgtcgt caagatcttc ccgggcgaac tgtttgggcc gaagatcatc aaggcaatca
     3121 aggggcccat tccctatgcc ccgatgatgc ccaccggcgg cgtcagtctg gagaatgtgg
     3181 aggaatggat caaggccggg gctgtggccg tgggagtcgg cggcagcttg atcgccggtg
     3241 ccaagaaggg cgattacgcc tcgatcaccg ccattgccaa acagttcatt gaaaaaattc
     3301 aggccgcgcg ggcctaggag gaatcggaat catggcaagt gttgtttgtt tcggagagat
     3361 catgctcagg ctctcacccc cgggatattt gcgttttgtc cagaccgatt cgttcgacgc
     3421 ggtctacggc ggcggcgagg ccaatgtcag tgtttcgctg gccaattacg gaatggatgc
     3481 cgcattcgtg accaaagtgc cggacaaccc catcggccag gcggccgtga atgagctgcg
     3541 ccgctacggc gtggatgccg ggttcatcgc gcggggcggc gaacggctgg ggatttactt
     3601 cgcggagaaa ggcgcctcgc aacgcccttc caaagtggtt tacgaccgga agtattcttc
     3661 catctcccag gcaaagccgg aggatttcga ttggcccaag atcttgaagg gcgcccgctg
     3721 gttccatttc accggcatca cccccgctct gggagacaat gtggcggcca tcgccctcgc
     3781 agcctgcaag gcggcacggg atttgggagt cactgtcagc tgcgatctga attaccggaa
     3841 aaacctctgg tccaccgaga aagccggcaa ggtcatgggg gaattgatgc cctacgtcaa
     3901 tctgtgcatc gccaacgagg aggacgccga gaaggtcttc gggatcaagg cggccgatac
     3961 cgacgtc
//
LOCUS       DASVMB010000144         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_460773,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000144 DASVMB010000000
VERSION     DASVMB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460773"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..892
                     /locus_tag="VF531_04515"
     CDS             257..892
                     /locus_tag="VF531_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713262.1"
                     /translation="MRLKWGIGLLLTGVMIFGLASAAFGYGTLGETDQMYTPTPATLS
                     QGSLGIGVNFAQDSLSYFNFDYGLVPDLEAGVAVFNYPDNTRITFRGKFRLLREDKDT
                     PSLTLGIEDIGKDEELSPYLTVGKTFSDVGIKGYIGVGGGGFDGVFGGLSKTFNTGSS
                     RGSLRSVELYLEADSHALNLGTKLQVGSQTKINFGLVDMERWIFGATFLIK"
     gene            complement(1026..2003)
                     /locus_tag="VF531_04520"
     CDS             complement(1026..2003)
                     /locus_tag="VF531_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7713263.1"
                     /translation="MSDPVIYSIIVPVYNEEDVVTETYHRLKLVLERVDEPYEMIFVD
                     DGSHDRTALLLKRLAVEDPGLRLIEFSRNFGHQLAITAGMDFASGRALVVIDGDLQDP
                     PEVIPRLIAGWKAGYDVVYAQRRERQGENWFKKFSARCFYRLLRRMTDVAIPVDTGDF
                     RLIDARVNRELQTIRERNRYIRGLISWLGFRQIGIVYIRDERHQGKTKFSLGKMIHFA
                     LDAITSFSDKPLRIASYMGFFLSGASFVYLMVVLYQKIFTNSTVPGWASILAVNLFFN
                     GIILMILGIIGEYISRIYDEAKGRPLYVIRGLTNLPNRQDIKKDGGDYR"
     gene            complement(2040..5930)
                     /locus_tag="VF531_04525"
     CDS             complement(2040..5930)
                     /locus_tag="VF531_04525"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX7713264.1"
                     /translation="MKHHLPLKRAILVCLLGIIPGLSVSATAQSLPELTNGGFEQFDS
                     ARRTIGWMGDSWGSGSKFGITRTKSHRGQYAAFIESKSPNDARFIQTIPVSPETIYRF
                     SGWIATFGVPAGRFGANLCVMETGGYAHSRGINGQSNWQPVDLIFRTGPYQNQVTLGI
                     RLGFYGETLSGTAYFDDLELTRLTDSEIAYQTLGSTMPYQPAQSLIHPGLADPVPENG
                     EMPIISRIIGLFDYSAAIILFYLIFLGGLILKRHELNGLVMGQPHSGLLSPQQVHNRL
                     NLWFGVAAVLALGVRIPLFGAAPVPHDFAAFEYWAMRMADLGPARFYSPDIFCDYPPF
                     PLYLLGLCGWFIKILGGTGNHLLSSVLIKLPSYLSDLATAWLIFSLLRKKRPAFSFCL
                     ALLYLFLPPVIYNSAYWGQVDSYYAFLMLAAFCLLIKKKPEAATVLVAASFLTKAQTI
                     AFLPLFLLYLLLHFGWRTTLRAALAGLAAGILILLPVSQGHPVTWAVSHYLAQAGLYP
                     YASMNAANLWVLCSANFVNDATQVLPGVSYRLLGILLFLVGAGWSCYYYYRKPNPASL
                     AAGFAISALALFLFFPRMHERYLFPALAFLLLMVGYYRDKKIYLVTLIVSLSYFFNMH
                     AVVLKDYLRTLAEPSFSRIMYILALVNLLSFMALVLYLGVHLGKGTRPFKEFLHRYAV
                     ALRSGILAQRARGKIEQRPFHLTKRDYVLMGILILIYALFLFHRLGSWSTPRTGYPLK
                     QGELLEITLAGKANLSEVSWYSATGAGRLRIEAENGSGWSELAVISCDGVNDFYKLRE
                     LKVATLSVSKLRLVPLQSGWRLNELAFFDPEHRMIPVATVQPGPDKHPITAARHPLWD
                     EPERMVERYSYYNSTYFDEIYHGRTAWEFITGSPVYETTHPPFGKDWLSLGILLFGMN
                     PFGMRFMHVISGLGLIIMLFFLGRELLATRFGAYCTMLLGMLDFMPFVQSRYATIDST
                     SVLFIGLMFLFTFKYCREQEAQPGFRNSLATFVLIMFCFALAAATKWTGVYGLSGIAA
                     CILMVLIRQYRSYRQERAKLNFDEELAPARAALSKDFWRNRFGATVLGGILAFVMIVP
                     AVYYLSYIPFLKCKGIASPFSVDGVRTVLQNQKDMYDYHSKLTAPYAFESDWWSWPFD
                     FKPNWIYPNYPVAVRPGYKVSIVSMGNPLIWLLGLIALVVLVYQLLTLRKFSRFHLAV
                     IAFLSVYLPWILVTRAAVIYHFFPCLPLYLTFTALLLEPLWRLGKPGRRVIYTLFGCS
                     LGLLLLFYPVLIGVEVRQSFVDGFLRWFPRDWVF"
     gene            complement(5927..>6145)
                     /locus_tag="VF531_04530"
     CDS             complement(5927..>6145)
                     /locus_tag="VF531_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713265.1"
                     /translation="DEVKHRPRYLVAESFKIRRMDAATRRGRSCYSRPLFTIPGTVTH
                     REIPRFESHESQGRFGVSERWREEDPLS"
BASE COUNT         1645 a         1529 c         1634 g         1337 t
ORIGIN      
        1 gaggccgaag atcattacga ttatgacgcg gattataacc ccttccgctt aacctgattt
       61 cttcttccaa ccgcgccacc aatatccttt atcacctcat ctttgacaat accccggtat
      121 tttccaaaag ggatacggtt agactagtag aacctgatgt gcttagaaac cggtatcagg
      181 aaggctttct tactggctcg cccggaaaac actggggttc aacgtttttt acaatatcaa
      241 aatgaggtgg ttatgaatga gactcaaatg gggaatcgga ttgctactca ccggggtaat
      301 gatttttggt ctggcctccg cggctttcgg ttatgggact ttgggggaga ctgaccagat
      361 gtatacgcct actcccgcca ccctctctca gggatcattg gggatcgggg taaacttcgc
      421 tcaagatagt ctgagttatt tcaatttcga ttacggtctg gtcccggatc tcgaggccgg
      481 ggtggcggta ttcaattatc cggacaacac ccggattacc ttccggggca aattccgctt
      541 attacgcgag gataaggaca ctccgagtct gacccttgga atcgaggaca tcggaaagga
      601 tgaggaacta tccccatacc tcacggtggg caaaaccttt tcggatgtgg gcatcaaagg
      661 ttatatcgga gtagggggcg gcggtttcga cggtgttttc ggcggactga gcaaaacctt
      721 taataccggt agctcccggg ggagtctgcg ttcggtcgag ctttacctgg aggcggattc
      781 ccatgcttta aatctcggca cgaaactcca ggtaggcagc cagacgaaga tcaacttcgg
      841 cctggtcgac atggaaagat ggatcttcgg cgcaaccttc ctgattaaat aaggtttctt
      901 caattccgtt cgtatggtgc ggtggggatt tccgaaactc cacgggaact ggacgaatcg
      961 gattcaggtt aattcgagta ccgggaagag ctgaacttca gagagacccg agagatggcg
     1021 ggtggttagc gataatcacc gccatctttt tttatatcct gccggttcgg aagattggtt
     1081 agacccctga ttacatatag gggacggcct ttagcctcgt cataaattct tgagatgtat
     1141 tcgccgataa tccccaggat catcaggatg atgccgttga agaaaagatt gaccgcgaga
     1201 atcgacgccc aaccgggtac ggtactattg gtgaatattt tctggtagag tacgaccatc
     1261 aaatagacga aactcgcgcc ggaaagaaag aaccccatat aggaagcgat tcttaacggt
     1321 ttatcggaaa aggaagttat tgcatccagt gcaaaatgaa tcattttgcc caaagaaaat
     1381 ttggttttcc cttgatgacg ctcgtcacgg atataaacaa tcccgatttg cctaaatccg
     1441 agccagctga taagtccgcg gatgtaacgg ttgcgctcgc ggatcgtttg aagttcccgg
     1501 ttgacccggg catcaatcaa ccggaaatca ccggtgtcta ccggaattgc cacgtccgtc
     1561 attcttcgca gcagacggta gaaacagcgg gcactgaatt ttttaaacca gttctcgccc
     1621 tgcctctctc tgcgctgcgc gtaaaccaca tcgtaaccag ccttccaccc tgcgataagc
     1681 cttgggataa cttccggcgg gtcctgtaga tcgccgtcga tcaccaccaa tgcgcggcct
     1741 gaggcaaaat ccattccggc ggtgatcgcc agctggtggc cgaagttacg ggaaaattcg
     1801 atcagccgca gccccggatc ctctacagcc agcctcttta ataagagggc ggttcggtca
     1861 tggctgccgt cgtctacaaa gatcatttca tagggttcat caactctttc caggacgagc
     1921 ttcagacggt gatatgtctc ggtcactacg tcttcttcgt tgtataccgg aacgataatt
     1981 gaataaatta cgggatcaga catatctgta ctcctcctca ttaatttcag agagcctgat
     2041 taaaagaccc agtcccgcgg aaaccatcgt aaaaagccat ccacaaagga ctgccgtacc
     2101 tcaaccccga taagtacagg ataaaagagg agcagcaagc caagggaaca tccaaacagc
     2161 gtataaatta cccggcgtcc gggttttccc aacctccaaa gaggttctaa gagcagcgcg
     2221 gtaaaggtca ggtacaatgg cagacaggga aagaaatgat agattacagc cgcccgggtc
     2281 accaagatcc aaggaagata gaccgagaga aacgcgatga ccgccaggtg gaatcgggag
     2341 aacttccgta acgtcaataa ctggtatacc aaaaccacca aagcaatgag gccgagcaac
     2401 caaatgagcg ggttccccat gctgacaatg gagactttgt accccggtcg gaccgccacg
     2461 ggataattgg gatagatcca gttgggcttg aaatcaaacg gccagctcca ccagtccgac
     2521 tcaaaggcat agggagcagt cagtttggaa tggtaatcgt acatatcctt ttggttttgc
     2581 agaacggttc tcaccccatc taccgagaag ggcgaggcta ttcccttgca ttttaggaat
     2641 ggaatataag atagatagta gactgccggc acaatcatta caaatgccaa tattcctccc
     2701 aaaactgtag ccccaaagcg attccgccaa aaatccttgg aaagggccgc gcgcgcgggc
     2761 gccaattctt cgtcgaaatt aagcttggca cgttcctgcc ggtaggatcg gtactgacgg
     2821 attaatacca tcaggatgca ggcagcgata ccggagagtc cgtaaacacc ggtccatttg
     2881 gtggctgcgg ccaaggcaaa acagaacatg atcaagacaa acgttgccaa agagttccga
     2941 aaccccggtt gggcttcttg ctcgcgacag tatttgaagg tgaacaggaa catgagaccg
     3001 ataaagagca ccgaggtgct gtcaatggtg gcgtagcggc tttggacgaa cggcatgaaa
     3061 tcgagcattc ccaggagcat ggtgcaatat gctccaaagc gggtggcaag aagttcgcgg
     3121 ccgaggaaga aaagcataat aattaggcct aatccggata taacatgcat aaatcgcata
     3181 ccgaaggggt tcatcccgaa aagcagaata cccaggctta accagtcctt accgaagggt
     3241 gggtgggtgg tttcatatac cggactgccg gtgataaact cccatgcggt acggccatgg
     3301 taaatctcat cgaaataagt gctattgtag taggaatagc gttctaccat tcgttccggt
     3361 tcatcccaga gcgggtgacg ggctgcggta atagggtgct tatcgggacc gggttggacg
     3421 gtggccaccg ggatcatccg gtgctcagga tcaaaaaagg ccagttcgtt caggcgccat
     3481 ccggactgta gtggaaccag acgtaatttg ctaacggaca gagtcgcgac ttttaattcc
     3541 ctcaacttat aaaaatcgtt caccccgtcg caggagatta cggccaactc gctccaaccg
     3601 gatccgttct cggcttcgat cctcaagcgt ccggctccgg ttgcgctgta ccaggagact
     3661 tcactaaggt ttgctttacc tgccagtgtg atctcaagaa gttccccttg ttttagggga
     3721 taacccgtcc gcggtgtaga ccaggaacct aggcggtgaa agaggaaaag ggcatagata
     3781 agtatcagga ttcccatcaa tacgtagtcc cgtttggtga ggtgaaacgg gcgctgttca
     3841 attttgccgc gtgcgcgctg ggctaggatt ccggagcgaa gcgctacagc ataccggtgc
     3901 aagaactctt tgaaggggcg ggtgcctttt ccgaggtgca ctccgagata gagaacgagt
     3961 gccatgaagc tcagcaaatt aaccaaggcc aggatataca tgatccgact gaaacttggt
     4021 tccgcaaggg tccgaagata gtccttcaag accaccgcat gcatgttaaa gaaataactc
     4081 agactgacaa tcaacgtgac caggtagatc tttttatccc gatagtaccc gaccattaac
     4141 agcagaaaag ccagcgccgg aaagaggtaa cgctcatgca tcctggggaa aaagagaaag
     4201 agtgccagag cactgatggc gaaacctgcc gctaaactgg ccgggttggg tttgcggtaa
     4261 taatagtagc agctccatcc ggcgccaacc aggaaaagca gaatccccag aagccggtag
     4321 ctgactcccg ggagtacttg ggtggcgtcg ttcacaaaat ttgcactgca cagcacccag
     4381 agattggcgg cgttcatgga ggcatacggg taaagaccgg cctgcgccag ataatggctg
     4441 acggcccagg tcacggggtg gccttgactt accggcagca aaatcaggat cccggctgcc
     4501 aatcccgcca gagccgctct cagggtggta cgccacccga aatggagcag caggtaaagc
     4561 aggaacagcg gcaggaaagc gatggtctgg gccttagtca aaaaagaggc ggccactaaa
     4621 accgtagccg cttccggttt ttttttgatc aggagacaga aggcggcgag catcaaaaaa
     4681 gcataatagg aatccacctg cccccaatag gcggaattat agatcaccgg cggtaaaaac
     4741 aagtaaagca atgccaggca gaatgagaag gccggacgtt tcttccttag cagactgaag
     4801 atgagccagg ccgtggctag gtcggacagg taggaaggaa gtttgattaa tacactcgac
     4861 aacaggtgat ttccggtacc gcccaggatt ttgatgaacc aaccgcagag tcctaaaaga
     4921 tacaagggaa acggcggata atcacaaaaa atgtctggag agtaaaaacg ggccgggccc
     4981 aaatccgcca tacgcattgc ccaatactca aaagccgcaa aatcatgggg caccggggcc
     5041 gcaccgaaga gtgggatccg gacgccgaga gccaaaacgg cggctactcc gaaccagagg
     5101 ttcaagcggt tatgtacttg ttgaggcgac aggagaccgc tatgaggttg tcccatcacc
     5161 aacccgttta actcatggcg cttcaggatc agtccgccta gaaatatcag gtaaaacagg
     5221 atgattgccg ctgaataatc aaataacccg atgatccgtg aaatgattgg catttctcca
     5281 ttctctggga cgggatcggc caatccgggg tggattaaag actgtgctgg ctgatagggc
     5341 atggtagagc caagagtctg gtatgcaatc tccgaatcgg tcagtctcgt taattccaaa
     5401 tcgtcaaagt aagcggttcc cgaaagggtt tctccgtaaa aacccagccg gattcccagg
     5461 gtcacctgat tttgataggg cccggtccga aaaatcaagt ctaccggttg ccagttactt
     5521 tgaccgttaa tcccccggga atgtgcataa ccacccgttt ccattacaca taaattggct
     5581 ccgaatctgc ctgccggcac gccgaaggtg gcaatccaac cggaaaaacg gtagattgtt
     5641 tccggagaga ccggtatggt ttgaatgaaa cgcgcgtcat tcggggattt ggactctatg
     5701 aatgcggcgt attgcccgcg gtgagacttg gtgcgggtga tgccgaactt ggacccggaa
     5761 ccccaggagt cacccatcca gccgatggtt ctgcgggcgg agtcgaattg ttcaaaaccg
     5821 ccgttggtaa gctccggaag ggactgcgct gtggcagaca cactcaaacc tggaatgatt
     5881 cccaacaggc aaaccaggat ggctctcttc aaaggaagat gatgtttcat gataacgggt
     5941 cctcctcccg ccatcgttcg gaaactccga atcgaccttg ggattcgtga gattcaaaac
     6001 ggggaatctc acgatgggtt acagttcctg gtatcgtaaa tagagggcgg gagtaacaac
     6061 ttcgccccct ccgggtcgca gcgtccatgc ggcggatttt aaaactctcg gcaaccagat
     6121 accgtggtct gtgttttacc tcatc
//
LOCUS       DASVMB010000145         7097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_165460,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000145 DASVMB010000000
VERSION     DASVMB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165460"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VF531_04535"
     CDS             complement(<1..567)
                     /locus_tag="VF531_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7713266.1"
                     /translation="MFLYNIKTRLLICFVFILAVFAMVQILLLGSGPILHFIIAGIFI
                     AIFWVAGNHLFELWVTNPIRQMIVFTQQVSKKDLSQKLSLKTKDEYLLILEESLNEML
                     HNLQVILEENLEASEQLALAASEMSSMAEQANCATEEITSTMGQMSKVTQEQFENVNV
                     SVSASQQMSETAQQVATEAQKAASFSSQA"
     gene            complement(754..984)
                     /gene="secG"
                     /locus_tag="VF531_04540"
     CDS             complement(754..984)
                     /gene="secG"
                     /locus_tag="VF531_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7713267.1"
                     /translation="MKLVLQILLLIISVALVTIIIIQPSKGEGLGAIGSGGQVFFAKN
                     KGLEKLLDKATTWLAVVFGLLLMLVELYDKIF"
     gene            complement(1218..2525)
                     /gene="eno"
                     /locus_tag="VF531_04545"
     CDS             complement(1218..2525)
                     /gene="eno"
                     /locus_tag="VF531_04545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7713268.1"
                     /translation="MTTIVDVYGREILDSRGNPTVEVEVELADGSVGRAAVPSGASTG
                     AFEAVELRDGDKHRYLGKGVQKAVDNVNDEIAGEVVGLDALDQVTVDKVMMELDGTPN
                     KAKLGANAILGVSLAVAKAAASSMGLPLYRYLGGVNAKELPVPMMNILNGGKHADNSV
                     DLQEFMIMPVGAESFKECLRWGAEVFHALKKVLKEKDYGTAVGDEGGYAPNLKSNEEA
                     LEMIMAAIETAGFKPGQEIKIAMDPASTELWDSAKEKGEDGKYYFWKSKMMKTSAEMV
                     DFYAKLVENYPIISIEDGMAEEDWDGWKQLTERLGDRVQLVGDDLFVTNTERLSKGIQ
                     KGVANSILIKVNQIGTLTETLDAIEMAKRAGYTAIVSHRSGETEDTTISDIVVATNAG
                     QIKTGAPSRTDRVAKYNQLLRIEDLLADEAIFAGQKAFYNIAR"
     gene            complement(2543..4093)
                     /gene="gpmI"
                     /locus_tag="VF531_04550"
     CDS             complement(2543..4093)
                     /gene="gpmI"
                     /locus_tag="VF531_04550"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX7713269.1"
                     /translation="MGYRPAALIILDGWGVNPREEGNAIKKANTPFLDSLFSNYPHAT
                     LLCSGEAVGLPEGQMGTSEVGHMNIGAGRVVYQSLVRISKALREGELKRNPVFLDLVN
                     YAVREDRPLHVMGLVSPGGVHSHTEHLYGLIQLAKESGVKEIYIHAFLDGRDTPPSSA
                     REYLSELEDVCREKEIGRIATVSGRYYAMDRDKRWDRVEKAYRAMVAGEGEEADSALQ
                     AVSNSYAREITDEFVLPTVITHGGKPMATIGSGDPVLFFNFRPDRAREITRAFIEENF
                     NSFERPKGYLNPHFVCMTQYDESLDVPLLFPPEAKLKNILAEVLADHGLKELRIAETE
                     KYAHVTFFFNGGVEQPYSLEDRVLIPSPKVATYDLQPEMSANQVTENLMTQIEQDKYD
                     AIIVNFANCDMVGHTGIMEAAMKAVETVDRCLSRILPAILAKGGAVIITADHGNAEQM
                     IDYQTGEPYTAHTTFPVPVIVAGVKPPYKVRDGILADLAPTILEMLSIAKPPEMTGTS
                     LIERTNGK"
     gene            complement(4341..5090)
                     /gene="tpiA"
                     /locus_tag="VF531_04555"
     CDS             complement(4341..5090)
                     /gene="tpiA"
                     /locus_tag="VF531_04555"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7713270.1"
                     /translation="MRKPIIAGNWKMYKTIPEAKQLVSGVIKELADFSGVEVVFCPPF
                     TALSTVKQLIQGTSFGIGAQNLYWKEQGAFTGEISPLMLKDIGCDYCIIGHSERRQYF
                     GETDTTVNQKVKAALAVGIKPIICVGETLAQREAGETNSLIKSQTEQALAGVAPDVVP
                     EIVIAYEPIWAIGTGKSSTPKDASGVIGLIRKTVAAMFGNDAAQKLRIQYGGSVKPEN
                     IKEFMNEPEIDGALVGGASLEIDSFIKIVKY"
     gene            complement(5152..6339)
                     /locus_tag="VF531_04560"
     CDS             complement(5152..6339)
                     /locus_tag="VF531_04560"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7713271.1"
                     /translation="MNKLSIDDLNVKGKRVLVRVDFNVPVDDSGKITDDRRIRAALPT
                     IKKLSESGAKVVLVSHFGRPKGGPDNKYRMDNIGKRLSELLGKPVVKVNDSIGDEPKK
                     AIDNLAWGGIVLLENVRFYKEEEANDEAFAKKLAELADVYVNDAFGTAHRAHASTAGV
                     AKFLKPAAAGYLMQKEISVMGKALTNPERPFVAILGGAKVADKLGVIKNLLEKVDSLI
                     IGGGMAYTFLKAQGLEIGKSLLDSEKIDFAKEMMAKAKAKGVKLLLPVDTVVTDDFKN
                     PTTIKTVQANQIPADLEGVDIGPETVRLFASAITGAKTVIWNGPMGVFEMKQFATGTR
                     AIAEALTKCKGTTIIGGGDSAAAVEQMGFADQVTHVSTGGGASLEFLEGIELPGVAAL
                     TDK"
     gene            complement(6629..>7097)
                     /locus_tag="VF531_04565"
     CDS             complement(6629..>7097)
                     /locus_tag="VF531_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7713272.1"
                     /translation="YTNDQMILDLPHSDLRRARAAALSMIPTTTGAAKAVSLVLPELK
                     GKLNGFAIRVPTPNVSVVDLVADLEQSATAEAINQALQEAASGPLRGILAYSEEPLVS
                     KDFNGDPNSSIIDALSTMVIDGNMAKVVSWYDNEWGYSNRVADLASYIVKKGL"
BASE COUNT         1738 a         1952 c         1603 g         1804 t
ORIGIN      
        1 ggcttgtgat gagaaactgg ccgccttttg cgcctcggtt gcgacttgtt gcgcggtttc
       61 cgacatctgt tgagaagccg acaccgatac atttacattt tcgaattgtt cctgagtcac
      121 cttggacatt tgacccatgg tgctggtaat ctcttccgta gcacaattgg cctgttccgc
      181 catggaagac atctccgatg cggccagggc cagttgttcg gaagcttcaa ggttctcctc
      241 gaggatgact tggagattat gtaacatttc atttaaggac tcctcgagga tcaagaggta
      301 ttcatccttg gtcttcaaag acaatttctg gctcaaatcc ttctttgaca cctgctgggt
      361 aaagacgatc atctgacgga tcgggttggt gacccaaagt tcaaagagat gattgcctgc
      421 cacccaaaag atggcgataa aaattcccgc aataatgaag tgcaaaatcg gtccggaacc
      481 taaaagtaaa atctgcacca tagcaaagac tgccaatata aaaacaaaac aaattagcaa
      541 tcgggtttta atattgtaca aaaacatgaa aaagaacctc ccccttatac ggcctattat
      601 ttaaattcga caaacccggc cgatttcctt caacgtcaag tttccgattc aaccgcatca
      661 attgatgaaa tcgatggatg aattatcctt taaccaggcg ctagaagacg gtggaatttg
      721 caatggtttt cgtaaaaaaa aagggcttgc gccctaaaaa atcttgtcgt ataactccac
      781 cagcatcaga agtaatccga aaaccaccgc cagccaagtt gtggctttgt ccaacaactt
      841 ttcgagcccc ttattcttgg cgaagaaaac ctgaccgcct gagccgatgg ctcccagacc
      901 ttcaccttta ctcggttgaa taatgatgat ggtcaccagg gcgaccgaga tgatcagtaa
      961 aagtatctga agaactaatt tcaaaataac acctcccacc cataattcac ggttttcgaa
     1021 aaaccagaat cttcgcaaat cgttaactta aatattgtag cataaagcgg acaggaagtg
     1081 caagtgttaa tgaacgattc gtccggacct tttttatttt tcccggccaa aacagcggat
     1141 ctcaactcat gaatcactcc ggaagcaata tccttgccgc cggagtgact tcaccgcttt
     1201 cagggaagga ccaccgctta acgggcgata ttatagaaag ccttttgacc tgcgaagatc
     1261 gcctcgtccg ccaagagatc ctcgatacgc agcaattgat tatacttggc aacccggtct
     1321 gtacgggagg gggcgccggt tttgatctgg ccggcattgg tagctaccac aatatcgctg
     1381 atggtagtat cttctgtctc accggaacgg tgggagacaa tcgcggtata acccgcccgt
     1441 ttagccattt cgattgcatc gagggtctca gtcaaggtgc cgatctgatt cactttaatg
     1501 agaatcgagt tggccacacc tttttgaatc cccttactca gccgttcggt attggttacg
     1561 aagagatcgt caccgaccag ttgaacccta tcacccaagc gctcggtcag ttgtttccaa
     1621 ccatcccagt cctcttcggc cattccgtct tcaatagaga tgatcgggta attctcgacc
     1681 agtttggcat agaaatcaac catctctgcg gaagttttca tcatttttga cttccagaaa
     1741 taatactttc cgtcttctcc tttttctttg gcggaatccc agagctccgt agatgcggga
     1801 tccatggcga tcttgatctc ctgtcccggt ttaaaaccgg cggtctcaat tgcggccatg
     1861 atcatttcca aggcctcttc attacttttt agattggggg cgtacccgcc ttcatcccca
     1921 actgccgttc cataatcttt ctcttttaag actttcttga gtgcatgaaa aacctcggcg
     1981 ccccaacgaa gacattcctt aaaactctcc gcccctaccg gcatgatcat aaactcctgt
     2041 aaatcaacgc tattatccgc atgttttccg ccgtttagga tattcatcat cggtaccggc
     2101 aactctttgg cgttaacacc acctaaatat cgatagagcg gtaaacccat gctggaagcg
     2161 gcggctttgg ctactgccag ggatactcca agaatggcgt tagcccccaa cttggctttg
     2221 ttgggtgtac catccagttc catcataacc ttatccacag tcacctggtc gagggcgtcg
     2281 agccccacta cttcaccggc gatctcatca ttgacgttat caaccgcctt ctggactcct
     2341 ttgcccaaat aacggtgttt atcgccgtcg cgaagttcca ctgcctcgaa agcacctgtg
     2401 gaagctcccg aaggtacagc ggctcttccg acactgccgt cggccaactc cacttccact
     2461 tccaccgtgg gattgccgcg cgagtccaaa atctcgcggc catagacatc aacaatcgtg
     2521 gtcattgaaa acccccctca ttctattttc cattagtccg ctcaatcaac gaagttccgg
     2581 tcatttccgg aggtttggca atcgaaagca tctcaaggat cgtcggagcc aaatcggcca
     2641 aaattccatc ccgaacctta tagggcggct taactccggc tacgatgacc ggaaccggga
     2701 atgtggtatg agccgtatag ggctcaccgg tctggtagtc gatcatctgc tcggcattac
     2761 catgatccgc ggtgataatt actgctccgc ccttggccaa aatcgccggg aggatccggg
     2821 acagacaacg atcgaccgtt tcaacggcct tcattgccgc ctccataatc ccagtatgac
     2881 cgaccatatc gcagttggca aagttaacaa tgatcgcatc atatttgtcc tgctcaatct
     2941 gggtcatcag attttccgtc acctgattgg cgctcatctc cggctgcaga tcatacgtgg
     3001 caaccttcgg tgaaggaatc aatacgcgat cctcaaggga atagggctgt tccactccgc
     3061 cgttaaaaaa gaaagtcaca tgggcatatt tttcagtctc tgcaatccgt aattctttca
     3121 gaccgtgatc tgccaaaacc tcggccagaa tgttttttaa cttggcttcc ggtgggaaga
     3181 gcaatggtac atccaggctt tcatcatact gcgtcataca gacaaaatgg gggtttagat
     3241 atcctttcgg ccgctcaaag gagttaaaat tttcctcaat gaatgcccgt gtaatctccc
     3301 gggcacggtc gggacggaaa ttgaaaaaga gtaccggatc acccgatccg atcgtagcca
     3361 tcggttttcc accatgagta atcacggtcg gcaaaacgaa ttcatcagtg atctcccggg
     3421 catatgagtt gcttacagcc tgaagcgctg aatcagcttc ctctccttca ccggctacca
     3481 tggcccggta tgccttttcc acacggtccc aacgtttatc gcggtccatc gcatagtacc
     3541 tgccgctgac cgtagcgatc cggccgatct ctttctcacg gcagacgtct tcaagctcac
     3601 tcaagtattc ccgggcactg gagggcggtg tgtcccgtcc atccaaaaac gcgtgaatat
     3661 agatctcttt caccccggat tccttggcca actggatgag accatataaa tgttcggtat
     3721 gactgtggac accaccgggt gataccagac ccatcacatg caagggacga tcttcgcgga
     3781 ccgcgtagtt tacaagatcc aaaaaaacgg gattccgttt caactcccct tcccgtaatg
     3841 ccttggagat tcggaccaga ctctggtata caacccggcc ggctccgatg ttcatatgtc
     3901 ctacttcgga ggtgcccatc tggccttccg gcaaacccac cgcctctccg gagcaaagta
     3961 atgtggcgtg aggataattg gaaaacaggc tgtccaaaaa gggagtgttt gcttttttga
     4021 tagcattccc ttcttctcga ggattcactc cccatccgtc tagaataatt aacgccgctg
     4081 gtcgataacc cattgctgcc tccctatatt cgcgattcgc cccatgggcg attacgcagg
     4141 tatgtgcgtg attgcccaca cgcgcgcata taatccggcg gatgtgccgg atcaaactat
     4201 ctccgagtca agccaaacaa cgcgggttcg attctatccg cggatcttcc gatccgcgga
     4261 tcaaaagtcg agaatgattg tagatatgat atcgcaatat ggctctaccg tttatcaggg
     4321 acaaatccaa aaaacccggc ttaatacttt acgatcttga tgaaggaatc aatttcgaga
     4381 cttgctccac caaccagagc gccgtcaatt tccggttcat tcatgaattc cttgatattc
     4441 tcgggtttga cacttccgcc atattggatg cggagctttt gtgccgcatc atttccaaac
     4501 atagcggcca cggttttccg tattaacccg atcaccccgc ttgcatcttt gggagtggac
     4561 gacttcccgg tgccgatggc ccaaatcggt tcataggcga tgacgatctc cggcaccacg
     4621 tccggagcta ctcctgccaa tgcctgctcg gtttggctct taatcaggga attggtttcg
     4681 cccgcttccc gttgggcaag ggtctcaccc acacagatga tgggtttaat ccccactgcc
     4741 agggcggcct tcactttctg attgactgta gtgtcggttt ccccgaaata ctggcggcgc
     4801 tccgaatggc cgatgatgca gtaatcgcaa ccgatatcct tcagcattaa aggagagatc
     4861 tctcctgtaa aagcaccttg ttccttccag tagagattct gggcgccaat tccgaaggaa
     4921 gtcccctgga tcaattgctt cactgtgctt aacgcggtga aaggcgggca aaagaccact
     4981 tccacaccgg agaaatccgc cagttctttg attaccccgc taactaactg tttcgcttcg
     5041 ggaattgtct tgtacatctt ccaattcccg gcgatgattg gtttacgcat tgaaccctcc
     5101 tgtatattcg tttcgtgaag ttgcctgata taagcaaaaa tcgggaacga attatttatc
     5161 ggttaatgct gccacacccg gcagttcgat accttcaagg aactccaggg aagcgccgcc
     5221 gccggtggaa acgtgagtga cttgatcggc aaaccccatc tgttcgacgg cagccgccga
     5281 atcaccgcca ccgatgatgg tggtcccttt acactttgtc aaagcctcgg caatggcccg
     5341 ggtaccggtg gcaaactgct tcatttcaaa tacccccatg ggcccgttcc aaattacggt
     5401 cttggcgccg gtgatggcgg aggcaaaaag cctaacggtc tccggcccga tatccacccc
     5461 ttccagatca gccgggattt ggtttgcctg aacggtcttg atcgtggtcg ggttcttgaa
     5521 atcatctgta actacggtat ctacagggag taacaatttc acgcccttgg ccttggcttt
     5581 cgccatcatt tccttggcaa aatcgatctt ttccgaatcg agcagagact ttccaatctc
     5641 aagcccctgg gctttcagga aggtatatgc catcccgccg ccgatgatca gggaatccac
     5701 tttttccagg aggtttttga tgacgcccag cttatcggca actttggctc cacctagtat
     5761 tgccacaaac ggacgttccg gattggtaag ggccttaccc atcacactga tctctttttg
     5821 catcaaataa ccggctgccg cgggtttcaa gaatttagcc acccccgcgg tagatgcgtg
     5881 ggcccggtgg gcggttccaa aggcatcatt cacataaaca tcagccagct cggcgagttt
     5941 tttcgcaaat gcctcatcat tggcctcttc ctctttataa aaccggacat tctctaagag
     6001 tacaattccg ccccaggcca gattatcaat ggcctttttg ggctcatcgc cgatggaatc
     6061 gttcacctta accacaggtt tacccagaag ttcggagagg cgtttcccga tattatccat
     6121 ccggtattta ttatccggac cgccttttgg acggccgaaa tgggatacca aaaccacctt
     6181 ggcgccgctt tccgatagtt ttttgatagt gggcagtgcg gcccggatcc gccggtcatc
     6241 cgttatctta ccggaatcgt caaccggtac gttaaagtcg actcgaacca aaacccgttt
     6301 accctttaca ttaagatcat caatcgataa cttattcatc accagaagcc cccttaagat
     6361 tgcgcgcatg ggcgcgccaa gaatcggaaa acccacattt acagactaga ccaggaacgc
     6421 attaaaccgg gataccggtt ttccacattc cacccgccgc aatgaatatg aacgcgcttt
     6481 tccgatacag tcctccgcgc gggaggtcgg aggacgatac ctgaatcccg aacaatcagg
     6541 attatgattt atgaatccgg agcatcactt cccttgcaaa tcggtcgccc ctttggggca
     6601 accgggggtt gagccgcccg aaggcggctc aaagaccctt ttttactata tacgacgcca
     6661 aatcggcaac ccggttggaa taaccccatt cattatcata ccatgatacc actttggcca
     6721 tattgccatc gatcaccatt gtggaaagcg catcaatgat ggaagagttg gggtcaccgt
     6781 tgaaatcctt ggataccaag ggctcctccg aataagctaa gataccccgg agtggtccgg
     6841 atgccgcttc ctgaagcgct tgattaatcg cctcagccgt tgcgctttgt tcgagatcgg
     6901 ctaccaaatc aactacagat acattgggag tcggtacccg aatcgcgaaa ccatttaatt
     6961 tccccttgag ctccggtagg accagagaaa ccgccttggc cgccccggta gtggtgggaa
     7021 tcatggaaag agcggcggct cgtgctcgcc gtaaatcgga atgaggcaga tccaaaatca
     7081 tctgatcgtt ggtataa
//
LOCUS       DASVMB010000146         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_681974,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000146 DASVMB010000000
VERSION     DASVMB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681974"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..>1516
                     /locus_tag="VF531_04570"
     CDS             167..>1516
                     /locus_tag="VF531_04570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HEX7713273.1"
                     /translation="MNREGKSLKVLAFDYGASSGRAILGKFDGERLTLEEIHRFSNDP
                     VQAGAGLHWDILRLYHEMRQGILRYKQKGESDLAGMAIDTWGVDFGLLTASGELLGNP
                     YHYRDSRTDGMIEAACKRVSKTEIYRETGIQFLKFNTLYQLLALKLNDSPLLAQAETL
                     LFIPDLLAFFLTGEKRSEYTIASTSQMLNPTTGDWARMLLDKLAIPQSILAELVEPGA
                     VIGRIKPDLAADLGIPGFPVIAAAEHDTASAVVAVPAEVTDFAYLSSGTWSLMGVELP
                     APQINDTTFRLDYTNEGGIDRTTRLLKNIMGLWIFQECKREWDQPGTVTGFGELLGEA
                     ATAEPFGCLIDPDAEPFGSPGRMTEKIRNFCRETGQKLPGTRGEVVRCIMESLAMKYR
                     MALEGLEEITGHPISVLHVVGGGSQNEMLNQFTANATNKPVLAGPVEATAIGNLAVQL
                     "
BASE COUNT          375 a          365 c          437 g          339 t
ORIGIN      
        1 aaagaatacc tttttcataa gatcacctaa aaatagaata acatattccc aagaggtggg
       61 gaagatttgc ttttataata atcttagact tataattaaa catggtttgg atctcggaga
      121 acaggcaaac cggattaggc agatttgggg atgaggtgaa acgggaatga accgagaggg
      181 aaagagttta aaggtcttgg cgtttgacta cggcgcaagc agcggccggg cgatcttagg
      241 aaagtttgac ggggaacggt tgacgctgga agagatccat cgttttagca acgacccggt
      301 acaggccggt gccggcttac actgggatat cctcaggctt tatcacgaaa tgcgccaggg
      361 aattctccgt tacaagcaaa aaggggaatc tgatctggcc gggatggcca tcgatacctg
      421 gggggtcgat ttcggtctgt tgaccgcctc cggggaactg ctgggcaacc cttatcacta
      481 ccgggatagt cgcaccgacg ggatgatcga ggcggcctgt aaaagggttt ccaaaacaga
      541 aatctaccgg gagaccggaa tccaattttt gaaattcaat accctctatc aattactggc
      601 gctgaagctt aatgattctc ctttactcgc ccaggcggag acgttgcttt tcataccgga
      661 tctattggcc ttcttcctaa ccggcgagaa acgatccgaa tatacaattg ccagtacttc
      721 ccagatgttg aatccgacta ccggggattg ggcccggatg ctcttagata agcttgcgat
      781 cccccaatcc atcctggcag aactggttga gcccggggcg gtgatcggca ggatcaaacc
      841 ggatctggcg gccgatttgg gtattcccgg tttcccggta atcgccgcgg cggaacatga
      901 tacagccagt gctgtggtgg cggttccggc agaagtgact gattttgcct atttaagcag
      961 tgggacctgg tccctgatgg gggtggagct tccggcgccg cagatcaatg acacgacctt
     1021 ccggctggat tataccaatg agggaggaat cgatcgcacc acccggttac tcaagaatat
     1081 catgggactc tggatcttcc aggaatgcaa gcgtgagtgg gaccagcccg ggacggttac
     1141 cggcttcgga gagttactcg gcgaggctgc tacagcggaa ccattcggat gtctgatcga
     1201 tcccgatgcc gaaccctttg gctccccggg acggatgact gaaaagatcc ggaatttttg
     1261 ccgcgaaacc ggccaaaaat tgccggggac ccggggcgaa gtggtccgct gcatcatgga
     1321 gagcctggcg atgaagtacc gcatggcgct tgaagggtta gaggagataa ccgggcatcc
     1381 catttctgtt cttcatgtgg taggcggggg gtcccaaaat gaaatgttaa atcagtttac
     1441 cgccaacgcc accaataaac cggtgctcgc aggtccggtg gaagccacgg ctatcggcaa
     1501 tctggccgta caatta
//
LOCUS       DASVMB010000147         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_460980,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000147 DASVMB010000000
VERSION     DASVMB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460980"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="VF531_04575"
     CDS             <1..109
                     /locus_tag="VF531_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7713274.1"
                     /translation="SMSREMCRMRDEGVIDFHLASIRILDPEALKGMVE"
     gene            249..1484
                     /locus_tag="VF531_04580"
     CDS             249..1484
                     /locus_tag="VF531_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEX7713275.1"
                     /translation="MANLIRLIDVTNRDGVQTSRLGLAKLEKTIINLMLNEMGIYQSE
                     FGFPTTRHEINYLNANLELAAMNVLAPIKLEGWIRAVPGDVEFALKQVPGIKHLNISA
                     STSQQLINGKYQGRKTGRDVINDMVAAAKKAKELGIETLGVNAEDASRTELDFIIEFA
                     RAAKDSGADRLRYCDTLGYDDPFTIYDRICAVAKDVKIDIELHCHNDLGMAVACSVAG
                     AKGAIDSGVDAYINTTVNGMGERAGNADLVAVLLALLKSSGFSKQYQLDPAVDLSKSW
                     QLAKYASYAFDVPLCINQVGVGSNAFAHESGIHADGALKDRKNYELFDFEELGRGEPE
                     IIETGRMITTGEYGGIKGFRNVYDKLEIEFHDDDEAKKILDLVRYANVHTQKPLTEYE
                     LRFVAHYPEIARKIMTVTP"
     gene            1815..2333
                     /locus_tag="VF531_04585"
     CDS             1815..2333
                     /locus_tag="VF531_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7713276.1"
                     /translation="MPGGFWNIIISTLAVYLFIVIAIRVFGKKELSQLSIVDLVFFML
                     ISNAVQNAMVGPDTSLFGGLVAAATLFITNYLFKFLLYRFPKFGRLIQGEAILLVYKG
                     KLNHSKLREAKLTMDELMEAIREHGVSTVEEVDLAVLEMDGNISVLSNEFQHQTIRER
                     KLPKVEKPTSQE"
     gene            2419..3285
                     /locus_tag="VF531_04590"
     CDS             2419..3285
                     /locus_tag="VF531_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019912226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7713277.1"
                     /translation="MDWLERMNRALDYLEQNIEAPLDIQEVSRVACASPFHFQRMFHI
                     LTGVTVAEYVRKRKLTLAAQELAASNIRVLDVSLKYGYDSPESFAKAFRKVHGLNPSE
                     ARGRGMGLKAYPRISFHISVKGAEEMDYKIIEKQAFRVLGKGIRVTTRDGENLRRIPK
                     FWDEFCEDGSYDKICKLMDGKDLLGICMEFSPEQEELTYMIAAESGRGTADLPEGWMV
                     KEIPAETWAVFPSVGTLPDAIQKVWERIFSEWFPATGYEHADGPELEVYPQEGMNDPD
                     YRCEVWIPITKK"
     gene            3468..>3871
                     /locus_tag="VF531_04595"
     CDS             3468..>3871
                     /locus_tag="VF531_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713278.1"
                     /translation="MEKHTYTYRWVILGVFMAIMAIQQLLWITFASITSQAAAFYHVS
                     DLGIGMLSMVFMIVYIFVSIPASWVIDTYGFRTAVGLGAVLTGISGLLRGFFASSYMW
                     VMIFQIGIAIGQPLIVNAVTKVAARWFPIKERA"
BASE COUNT          947 a          830 c         1094 g         1000 t
ORIGIN      
        1 ctccatgtcc cgggagatgt gccggatgcg ggatgagggg gttattgatt ttcatttggc
       61 ttccatccgt attcttgacc cggaagcgct caaggggatg gtggagtaat acgaatcgga
      121 agttgcatgt atactgtact cttgagcaag gggtcttgat ctttcatgcg ttatactata
      181 ttgtttatta aaatttactt ttcggagacg gtagatttgg actttatctc taaggggagg
      241 tagtcaagat ggccaatttg atccgactta ttgatgtcac caatcgtgac ggggttcaaa
      301 cttcccggct gggactggcc aaactggaga agaccatcat taatctgatg ttaaacgaga
      361 tgggtattta ccagagtgaa tttggtttcc ccaccacgcg tcatgagatc aattacttaa
      421 atgccaacct tgagttggcg gcgatgaatg tattagcccc gattaaactt gagggctgga
      481 tccgggctgt tcccggtgat gtggaattcg ccctgaaaca ggtgcccgga attaagcatc
      541 tgaatatttc ggcttccact tcccagcagc tgataaatgg gaagtatcaa gggcggaaaa
      601 ccggccggga cgttatcaac gacatggttg ccgcggctaa aaaagccaag gagctgggga
      661 ttgaaacact gggggtcaat gccgaggacg cttcccggac tgaactggat tttataatcg
      721 agttcgcccg ggccgccaag gattcgggcg ccgaccgtct gaggtattgc gatactttgg
      781 gttacgatga tccctttacc atctatgatc ggatttgcgc agtcgccaag gatgtaaaga
      841 tcgatattga gttacactgc cataacgacc tggggatggc ggtggcttgt tcggtggcgg
      901 gagccaaggg tgcgattgac agcggggtgg atgcttatat taataccacg gttaacggaa
      961 tgggggaacg ggccggaaac gccgacttgg tcgcggtctt gctcgcgctc ttgaagtcga
     1021 gcggcttttc caagcagtat caactcgatc cggcggttga tctctcaaaa agttggcaac
     1081 tggccaagta cgcgtcctat gcattcgatg taccgctctg tattaatcaa gtgggcgtgg
     1141 gatccaatgc ctttgctcat gaatccggga ttcacgccga tggggcgctc aaagacagga
     1201 aaaattatga actcttcgat tttgaagagt tgggccgggg tgagccggag atcattgaga
     1261 ccggccggat gattaccacc ggcgaatacg gtggcattaa aggattccgt aatgtctacg
     1321 ataagcttga gatcgagttc catgatgatg acgaggccaa gaagatcctg gacttggttc
     1381 gctacgccaa tgttcacacc caaaagccgt taacagagta tgaactgcga tttgtggcgc
     1441 attatcccga gatcgcccgg aagattatga ccgtaacccc gtaggcgatt taggcaacag
     1501 gcaacaggca ataggcaata gggaataggc aagaggcatg agccgtgagg ttcaggcctt
     1561 tttgtgtttg atgaaatggt gggtcaaaag tcactagact ctggactttc gacagctaca
     1621 ttcagatttt agaccattat aagtgactta caatactctt gcctcgctcc tcatgcctcg
     1681 tgccttgatt gaatttagac attccgacat cagactttgg attttctgtg caggatcatg
     1741 gaaagaattt aagaaagggt tgcataagtt cgcgcatcct gtgaaatgat ttgctcggat
     1801 tgaggtgatc cttgttgccg ggtggctttt ggaatattat tatcagtaca ttggcggtgt
     1861 atctatttat cgtcatcgcc attcgtgttt ttgggaaaaa agagctatcg cagctatcca
     1921 tcgttgatct ggtctttttt atgttgatca gcaatgcggt gcaaaatgcg atggtggggc
     1981 cggatacctc gctcttcggg gggttggtgg cggctgcaac actatttatc acaaattatc
     2041 tgtttaagtt ccttttatat cggttcccca agttcggccg gctgattcaa ggggaagcca
     2101 ttcttttggt atacaaaggg aaattgaacc actccaagct tcgtgaggcc aagctgacca
     2161 tggacgaatt gatggaagcc atccgtgaac atggcgtatc cactgtggaa gaggttgacc
     2221 tggcggtgct ggagatggac gggaacatca gcgtgttatc caatgagttt caacatcaaa
     2281 ccatccggga acgaaaatta ccgaaagtcg aaaaacccac gtctcaggaa taatattcag
     2341 actgacagga aagagcacgg aaaaacagga cggattctgc ggtgtatgtt atcatcattg
     2401 cgaaaggggg tagaggcttt ggactggttg gaacgtatga atcgggcctt ggactacctg
     2461 gagcaaaata ttgaggcccc tctcgatatt caggaggtat cccgggtggc atgcgcctcg
     2521 ccgtttcatt ttcagcggat gtttcatatt ttgaccggcg ttaccgtggc ggagtatgtc
     2581 cggaaacgga aattgaccct ggccgcgcag gagttggctg cttccaatat ccgggtgctg
     2641 gatgtgtcgc tgaagtatgg ttatgactcc ccggagtctt tcgccaaggc cttccggaag
     2701 gttcacggtc tcaacccttc ggaggcgcgc gggaggggga tggggctcaa ggcttatccg
     2761 cgcatctcct ttcacatttc ggtgaagggg gcagaggaaa tggattataa gatcatcgag
     2821 aagcaagctt tccgggtgct gggaaagggc atccgggtga ctaccaggga cggggagaat
     2881 ttacggcgga tacctaagtt ttgggatgag ttttgtgagg acggttccta tgataagata
     2941 tgtaaactga tggatggtaa ggatctgttg gggatctgta tggagttcag ccctgaacaa
     3001 gaggagttaa cctatatgat cgccgcagaa tccggccgtg gtaccgcaga cctgccggaa
     3061 ggctggatgg tcaaggagat ccctgcagaa acttgggcag ttttcccttc cgtcggaacg
     3121 ttgcccgatg ccatccaaaa agtctgggaa cggatcttct ccgagtggtt cccggcgaca
     3181 ggatatgaac acgctgatgg accggaactg gaggtttatc cgcaagaagg aatgaacgac
     3241 cccgactatc gttgcgaggt gtggatccca atcacaaaga agtaggaaga taaattccca
     3301 atatgataag caggatcaaa cggcccgggt tgagcggttc cctcgggccg ttcaaataat
     3361 aaaggaaaaa ccccgccgcg aacgaattac taaccgaacg gttcagggag tataatcctc
     3421 atgagagccg tgttttcata attcacagca aacaggaggc tgcttcggtg gaaaaacata
     3481 cgtataccta tcggtgggta attctcgggg tttttatggc gatcatggcc attcaacagc
     3541 tactgtggat cacattcgcc tctatcacca gtcaagctgc tgcgttctat cacgtatccg
     3601 atctcggcat cgggatgttg tcaatggtat tcatgatcgt ctatattttc gtctcaattc
     3661 cggcctcatg ggtgattgac acctatggtt ttaggactgc ggtgggcttg ggtgcggtgt
     3721 taaccggaat atctggcctg ctgcgaggtt tttttgcctc aagttacatg tgggtgatga
     3781 tttttcaaat tgggattgcc atcggtcagc cgttgatagt aaatgcggtt accaaggttg
     3841 cggcacgttg gtttcccatc aaggagcggg c
//
LOCUS       DASVMB010000148         6362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_241928,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000148 DASVMB010000000
VERSION     DASVMB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241928"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF531_04600"
     CDS             <1..426
                     /locus_tag="VF531_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7713279.1"
                     /translation="ADELNLIKSYAEIEKARFGADLKVEFEIDEAVEVKIPSLSIQPL
                     VENAIHHGIRKKGGNGTVTITVQSTPEGILVDVTDDGAGIPPEKLEHLLQVDSGRGVG
                     LWNIDARLRKLFGHGLTIRSEPGKGTRVMFLIPPEVKPD"
     gene            423..1511
                     /locus_tag="VF531_04605"
     CDS             423..1511
                     /locus_tag="VF531_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713280.1"
                     /translation="MIKAIILDDEQPSVAKLERLLLESGLVEIVAKFTSPLAALEFIE
                     TNTIDAAFLDIEMPDMDGIELATRIIDLQEWIAVVFVTAYNQYAVEAFRLNALDYLMK
                     PVTAERLQETLNRIAAVRELRVKPATVRVQCFGKFTVTSGVSEVKFRTEKAQELLAFL
                     IDRSGEFAHRSEICDSLWEEYDGDRALIHFNTTLYYVKKALREQGVTVPIQHDRNSYR
                     FDAAGFDCDYCRFREFAARSGNVNRSNLMEYEEIAGLYNGDYLSGMQFTWIERNRQML
                     KDQFIRLLIGMAEYFKTTGNHQKTVEWMKQGLVHEPLHRELNYRLIEALVLMNDLISA
                     NRYHDIYRNEIERKLRREPDDGFKKLLG"
     gene            1761..4865
                     /locus_tag="VF531_04610"
     CDS             1761..4865
                     /locus_tag="VF531_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7713281.1"
                     /translation="METQRAANKWQVLLLSGMIIAGIVLTGILYLSQPMNGRIHPIDG
                     RLDLRDWNPGRLLSLNGEWDFYWQKLLTYRDLKLNQAPDLEARVYGVWNDYRIGGKKL
                     PGFGYATYRLQVVNAPRGITLALRIPTISTSYRLYIDNKLVSANGKVGINREQSEPQS
                     LPKIVEFTPDKTSFELIIQVANFTHARGGIWYPMALGTSERIWAGYRKIAVRDFLLIG
                     ALAVMAFYYLVIFLLRPADKSSLYFVLLCLMFISRTGVFGDYILNRLAPAMSYQVTTA
                     VACITLCWFPVIVLLFVAQLFPAETPSKALKAVLVYGGMMTAIFLFTPASFYTSLINL
                     VRTAAIVTGFYVIICLTKAFLNHKNDALLVLIWSLAVFISAGRIMLAQNNIIQSSYGQ
                     IAQLCLLILLLMQSLVLARRFATTFHDVEALSEKLLKLDKIKDEFLANTSHELRTPLS
                     GILGVTEATLRGSEGELTGGQKQNLSIIAASSRRMANLINDILDYSKLKHGDIRLNIK
                     PVKLDGMISTVVKVFQQLTKAKEYEIIYEIPAGLPPALADENRVVQILYNLLGNAVKF
                     TTRGYIKVEVKVIGKMLEVCVSDTGEGIPGDKLEDIFKSFEQLDTSLTRKHGGTGLGL
                     SITKQLVELQGGKIRAESTPGAGSAFYFTLPAASVLAGENANEYPAEDESSMALPAPA
                     YALELVAASFEEQPVTLEKAGTSTRVLLVDDDAVNLQSLGAILKIGGYAVITANSGKA
                     ALEELAGRCEYSLVVLDVMMPEMSGYEVCQKIRESKSHFDLPVLMLTAKTATADIVTG
                     FEAGANDYLPKPFEPEELLARVKTLVNLKTSVDKAITTEVAFMQAQIKPHYLYNTLNT
                     ISSFCDTDPGQARQLIEEFANYLRQSFDFKNLEMFVPITNEIGLIKSYVEIEKARFGA
                     NLQVEFAIDENLDVQIPPLSIQPLVENAIHHGIRKKGGSGTVRVTVKNRDEGLLVSVA
                     DDGSGIPPEKLTKILQDDVSQGIGLWNINFRLRKLFGKSLIIESEPGKGTRVMFTIPR
                     EVSG"
     gene            5243..>6362
                     /locus_tag="VF531_04615"
     CDS             5243..>6362
                     /locus_tag="VF531_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX7713282.1"
                     /translation="MKQKGAEKIHGVLMILFTLIVIAAISFAYVAAAPPDLITVTIHS
                     GDSFTAKLDEAAGGDRSSIASLKIVTNGYSLSSADFGSLKSMATNGAGRLTYLDLQEA
                     ACAYNQIPDGAFDNTGNNIILQKILLPNSITDIGNFAFYNCSGLTGGLNLPNGITSIG
                     GGAFYNCSGLTGSLNLPNSLIGIGNIAFSRCSGLTGSLILPANLEQINPGAFFGCSGF
                     SSLTIPNGVLSIGSDAFNGCSGFTGSLTIPDSVTSIDYNAFRDCSGFTGSLTIPAGIT
                     GIAEMVFANCSGFTGNLVIPNGVTSIGNGAFEGCSGFKGKLNIPDSVTSIGYYAFYGC
                     SGFTGSLTTPGSVTGIDFYAFYGCSGFTGSLIISDGVTS"
BASE COUNT         1599 a         1469 c         1693 g         1601 t
ORIGIN      
        1 gccgatgaac tgaatctgat caaatcctac gcggagatag agaaagcccg ttttggcgct
       61 gatttaaaag tggagtttga gatcgatgaa gccgttgagg taaagatacc ttcactgtcc
      121 attcaaccat tggtggaaaa cgcgattcac cacgggatcc gcaagaaagg cggcaatggt
      181 acggtcacca ttacagttca aagtactccg gaaggtatac tcgtggatgt gacggatgat
      241 ggcgccggga tccccccgga aaaactggaa catctccttc aggttgattc cggccgggga
      301 gtaggtttgt ggaatatcga cgcaaggctc cgcaagcttt ttggtcatgg acttactatc
      361 cggagtgaac ctggcaaggg cacccgggtc atgtttctaa tcccgccgga ggtgaaaccg
      421 gattgattaa ggcgattatt ctcgatgatg aacagccgtc cgtggcgaaa ttggagaggc
      481 tgctgttaga aagcggcctg gtggagatcg tggccaagtt taccagcccg ctggccgcgc
      541 tggaattcat agaaaccaat acgatcgatg ccgctttttt ggatatcgag atgccggata
      601 tggacggtat agagctcgct acccggatca ttgacttaca ggaatggata gccgttgttt
      661 ttgtaactgc atataatcag tacgctgtcg aagctttccg cctgaatgcg ctggattatc
      721 tgatgaagcc cgtaaccgcc gaacggctgc aagaaaccct gaaccgaatt gctgcggtaa
      781 gagagcttcg ggtgaaaccg gccacagtta gggtccagtg tttcggtaag ttcacagtga
      841 cgagtggagt ttccgaggtt aaatttcgca cggagaaagc ccaggaacta ctggcatttt
      901 tgattgaccg cagcggtgaa tttgcgcacc gcagtgaaat ctgcgatagc ttgtgggagg
      961 aatacgacgg cgaccgggcg ttgatccatt tcaataccac cctttactat gtgaaaaagg
     1021 ctttgcgcga gcagggagtg accgttccga tacaacatga tcggaacagc tacagattcg
     1081 acgccgccgg gttcgactgc gattactgcc ggttccggga atttgcggca agatctggaa
     1141 acgtgaaccg gagcaacctc atggaatatg aagaaatcgc ggggctctat aacggcgact
     1201 atctgtcggg tatgcaattt acctggatag agcgtaaccg gcagatgctg aaggatcaat
     1261 tcatccggct gctcatcggg atggcggagt atttcaaaac caccggcaat catcaaaaaa
     1321 ccgtggaatg gatgaagcag ggtttagtcc atgaaccttt gcaccgggag ctgaattaca
     1381 ggctgattga ggctttggtc ctcatgaatg atctcatctc agccaaccgg tatcatgata
     1441 tttaccggaa tgaaatcgaa agaaagctgc gtcgggaacc ggatgacgga tttaagaagc
     1501 tgctgggtta gtggaagatt cgagtccgcc aactgtccaa atggttcccg gtagcggttc
     1561 agtagctgtt aagtggcaag tgttactata gtaatagaaa gcttgtagtc cgctgattcc
     1621 aaaggccccc ggatacctga ttgcctggtg ggacgctacc ccttggtcac ttttgctagc
     1681 gtccaacctt attttctgtg ggaatgaaat tagcagaata tatcatggtc ggataaggaa
     1741 aaaacaaggg gaattatagg atggagactc aacgagcggc taataagtgg caagtactgc
     1801 ttctgtccgg tatgatcatt gccggaattg ttttgaccgg gatactttat ttatcgcaac
     1861 ccatgaatgg ccggatccat ccgatagacg gccgcttgga cctgcgcgac tggaaccccg
     1921 gcagactatt gtcattgaat ggagagtggg atttttactg gcaaaagctc ctgacttacc
     1981 gggatctaaa gcttaatcaa gcgcctgatc ttgaggctag agtgtacgga gtgtggaatg
     2041 actaccggat cggggggaaa aaactcccgg gattcggcta tgccacgtac cggctgcagg
     2101 tagtcaacgc tccccgagga ataactttag ccttaaggat tccaaccatt tcaacttcct
     2161 atagattata tattgataat aaactggtct cggccaacgg taaagtcggt atcaaccgag
     2221 agcagtccga acctcagtcc ctgccgaaga tagtggagtt taccccggac aaaaccagct
     2281 ttgagctcat cattcaagtg gctaacttta cccatgccag gggcggtata tggtatccca
     2341 tggccttggg cacctcggaa cggatttggg ccggatatcg gaaaattgcc gttcgcgatt
     2401 tcctgttgat cggcgcgctg gcggtgatgg ccttttatta tctggtgatt ttcctgttgc
     2461 ggcccgcgga taagagcagt ttgtattttg tattgttgtg cttgatgttt ataagccgga
     2521 ccggagtttt cggagattat attctcaacc ggctggctcc cgcgatgagt tatcaagtca
     2581 caaccgctgt tgcctgtatc acattgtgtt ggtttccagt cattgttctc ttatttgtgg
     2641 cacagctatt cccggcggaa accccttcaa aggctctcaa ggcggtgttg gtttatggcg
     2701 ggatgatgac ggcaattttc ttatttaccc cggcttcttt ttatacaagt ttgattaacc
     2761 tagttcggac cgcggcaatc gtcaccggtt tttatgtaat aatctgctta acgaaagctt
     2821 ttctaaatca taaaaacgac gccttactgg tcctgatctg gtcattggca gtgttcatca
     2881 gcgccgggcg gattatgttg gcccagaaca atatcattca atctagttac ggtcaaatag
     2941 cccaactctg tttattaatt ttgctgctga tgcagtcgtt ggtattggcg cgccgctttg
     3001 caaccacttt ccacgatgtc gaggctttgt cggaaaaact gttgaaactg gacaagatca
     3061 aggatgaatt cctggccaat acctcccatg aattgcggac ccctttaagc ggtatcctgg
     3121 gggtaaccga ggccacacta cggggttccg agggtgaact taccggcggg caaaagcaga
     3181 atctatcgat catcgctgcc agcagccgga gaatggcaaa tctaatcaat gatattctgg
     3241 attattccaa gctgaaacat ggcgacatcc ggctcaacat caaaccggta aaactggacg
     3301 gcatgatttc gacggtggtt aaagtttttc agcagttaac caaggcaaag gagtatgaaa
     3361 ttatctacga gattccggcc gggttgccgc cggcgctggc ggatgaaaac cgggtggtgc
     3421 agatcttgta caatctgctc ggcaatgccg tcaagtttac cacccgtggt tatatcaagg
     3481 ttgaggtcaa ggtcatcggt aaaatgctgg aggtctgcgt cagcgatact ggtgagggaa
     3541 tccccgggga caagctggaa gatatcttca aatcttttga gcagttggat acctcattga
     3601 cacggaaaca tggcggaacc gggcttgggt tatccatcac caaacaactg gtggaattgc
     3661 aaggagggaa gattcgggcg gagtctactc ccggagcagg ttccgcgttt tatttcacct
     3721 tgccggccgc aagtgttctt gcgggtgaga acgctaacga gtacccggct gaggatgaat
     3781 cgagtatggc cttacccgca cccgcatacg cacttgagtt agttgcggct tcctttgagg
     3841 aacaaccggt tacattggag aaggccggga ccagtacccg ggttttgttg gtggatgatg
     3901 atgctgttaa tctgcaatct ttgggagcca tcctaaagat cggtggatac gccgttatca
     3961 cggcaaatag cggcaaagcc gccctggagg aactagccgg gcgctgcgaa tattcgctgg
     4021 tggtgttgga tgtgatgatg ccggaaatgt cggggtatga ggtttgccaa aaaattcggg
     4081 agagcaaatc ccattttgac ctcccggtgc tgatgcttac ggccaagact gccacagccg
     4141 atatcgtcac gggctttgag gcgggggcca atgattacct gccgaagccc tttgagccgg
     4201 aagagttgct ggcaagggtg aaaacgcttg tcaatctgaa gacctccgta gataaggcca
     4261 tcaccacgga ggtggcgttt atgcaagcgc agatcaaacc gcattatctt tacaacaccc
     4321 tgaataccat ttcttccttc tgcgataccg atcccggaca ggcacgccag ttgattgagg
     4381 aatttgccaa ttacctgcga caaagctttg atttcaaaaa cttggagatg tttgttccga
     4441 taaccaacga gattggtctg atcaaatcct acgtagagat cgaaaaggcg cgtttcggtg
     4501 ccaacctgca agtggagttt gcaatcgatg aaaatctcga cgtccaaata cctccgcttt
     4561 ccatccaacc cctggttgaa aacgcgattc atcacggcat ccgaaaaaaa ggcggcagcg
     4621 gcacggttag ggtcacagtc aaaaatcgtg atgaaggcct tctcgtttca gtggcggatg
     4681 atggttcagg cattcctccg gaaaaattga ccaagatact tcaggacgat gtcagccagg
     4741 gaatcggact atggaatatt aatttcaggc tcaggaagct tttcggcaaa agcttgatta
     4801 tcgagagtga accgggcaaa ggcacccggg tgatgtttac gatacctcgg gaagtatccg
     4861 gataaggggt ttccacccct atccggcctt aagaggccac ctttccccgc acggagaaag
     4921 ggaaaagtcc ggctctgttg tcatgatcgc atcgaagcat ggtggttgca cgtccgatgg
     4981 acgtgactcc tccccgtgcg gggaggagtc gcgtggcttg cacgcaaaaa agtagagaga
     5041 acgaacccta tttcgaaaaa aattactttt attgttttac caaaatgttt gataaaccct
     5101 tggcctccaa gggttttatt cgtttctgca tcaactccgt ttagtgacgg ttaagtcgtg
     5161 gttgagtggc ggttaagcgg cgaacggtaa aatgaaaatg gaaaacttta aagtatgtat
     5221 tcgtaggaaa ggaaagatat tgatgaaaca aaaaggagca gaaaaaattc atggggtgct
     5281 gatgatattg ttcaccctca tcgtgatcgc cgcaatctca ttcgcgtatg ttgctgcggc
     5341 cccaccggat ttgatcaccg ttaccatcca ttcgggggat tcttttacgg ccaagttgga
     5401 cgaagccgcc gggggagatc gcagctccat tgccagcctg aagattgtca ccaacggcta
     5461 ctctctttcc agcgctgatt tcggcagcct gaaaagtatg gctacaaacg gcgccggacg
     5521 acttacttac ctcgatctcc aggaggcggc ttgcgcttac aaccagatac cggacggggc
     5581 tttcgataat accggaaata atatcatact ccagaaaatt ctattaccaa atagtattac
     5641 cgatatcggt aactttgcat tttataattg cagcggatta acaggcggtc tcaacctacc
     5701 aaacgggatc acttctattg gtggcggtgc attttataat tgcagcgggt taacaggcag
     5761 tctcaacctt ccaaatagct taatcggtat cggcaatatc gcattttcaa gatgcagcgg
     5821 gttgacgggc agtcttattc ttcctgccaa tctagagcag atcaaccccg gtgcattttt
     5881 tggctgcagc ggtttcagca gtctcaccat tcccaatggt gtattgagta tcggcagtga
     5941 tgcctttaat ggttgcagcg gcttcaccgg cagcctcaca attccggata gcgtaaccag
     6001 catcgattac aatgcattta gagattgcag cggattcacc ggcagtctca ctattccggc
     6061 cggcattacc ggcatcgcgg agatggtatt tgccaattgc agcggtttta cgggcaatct
     6121 cgtgattccg aacggcgtta ccagcatcgg caacggcgca tttgagggtt gcagcggttt
     6181 caagggtaag ctcaatattc ccgacagtgt aaccagtatc ggttattatg cgttttatgg
     6241 ctgtagcggc tttacgggca gtctcaccac tcccggtagt gtaaccggca tcgattttta
     6301 tgcattttat ggttgtagcg gcttcaccgg cagcctcatc atttccgacg gtgtaaccag
     6361 ta
//
LOCUS       DASVMB010000149         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_386889,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000149 DASVMB010000000
VERSION     DASVMB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386889"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF531_04620"
     CDS             <1..409
                     /locus_tag="VF531_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7713283.1"
                     /translation="SHVINFDLPNVPETYVHRIGRTGRAGASGVALSFCDLEEEPYLK
                     SINRMIAKPIRVVTDHPFLRSVSDPATSPVADSRVNRNKVVTTSGRRTGSNRSTGSFR
                     GRRPVRGFDKRSETGGSPSQREAGDSRTGRDRG"
     gene            1111..2016
                     /locus_tag="VF531_04625"
     CDS             1111..2016
                     /locus_tag="VF531_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX7713284.1"
                     /translation="MIKSFDALKKELPGSKPLRLVVVAAQETVVLDAVSEATKAGWVK
                     PIFITTGEVPPFNDFSDLAITIHHASSLQQAAELGTELLARGEADLMMKGLIPTADFL
                     RPLLNTEKHLIKTGRMLTHVGAISIPNRGRLTLISDAAIMINPDLRAKEIIIENAICV
                     ARALGYNPPKVAVLSALEAVNPKIPSSVEAGKLKEMSQSGRFGDCVVSGPLALDNALC
                     HDAAVTKGLNGDPVAGEADVLIFPELVSGNILYKSFSIIAGYPSAGVVMGAKVPIILT
                     SRADPPEAKVNSIALACFLAQNTPG"
BASE COUNT          533 a          478 c          513 g          563 t
ORIGIN      
        1 gtcccacgtc atcaattttg atcttcccaa tgtgcccgag acctatgtgc accggattgg
       61 ccgcaccgga agggccggcg cgagcggggt ggcgctctcg ttttgcgatc ttgaggagga
      121 accgtacctg aaaagtatta atcgcatgat cgccaaaccc atccgggtgg tgacggatca
      181 ccccttcctc cggagcgtat cggatcccgc aacttctccg gttgccgact cccgggtcaa
      241 tcgtaacaag gttgtgacca cttcgggacg ccggacaggc agcaatcgtt caactggttc
      301 attccgggga cggcgccccg taaggggttt cgataaacga tccgagaccg ggggcagtcc
      361 ctctcagcgt gaggccggcg actcccggac cgggcgtgac cggggttgac caaggccacg
      421 gtaaccagtt caaagatccg gagtctagtc atttcatcga ttcgtaaatg attcgcgaga
      481 tactcgtatt aaaaactcaa atcatcccgg gtttcagggg tgattttttt tattagcttt
      541 attctcaata aggttatcta cggaatcctt ttggcttatg atagataaaa tacttccatg
      601 gccgcaataa aaccattccc attttcgtgt cttttgtgtg tttcgttgtg aataaaaaag
      661 gcttacagat agtaactttt taaccacgaa aatcaccaaa tccacgaaat cattttttgc
      721 ttatgataaa aaaacacctc cataacagca ataaggcgag tttccatttt cgtgtctttt
      781 gtgtgtttcg ttgtgaataa aataggctta cagagaaact ttttaaccac gaaaatcacc
      841 aaatccacga aatcattttt gcttataata aaaaacacct ccatgacagt aataaggcgg
      901 gtttccattt ttggtgtctt ttgcgtgttt cgttgtgaaa tatgtaataa actggcagga
      961 atatatcttg taaactagaa cgattttcta tagtcctatg agtcctctca aggagagggg
     1021 gatgatgaag ccgggttgtt ggatatttag ttagactatt gatatatcaa tacagccttg
     1081 aatatatgat tgaatcatgg atgtgattga atgattaaga gttttgatgc attaaaaaaa
     1141 gagttgcccg gaagtaaacc cttgcgactg gtggtggtgg ctgcccagga aacggtggta
     1201 cttgacgcag tgtcggaggc aaccaaagcg ggttgggtaa agcctatctt cattactacc
     1261 ggtgaagttc cgccattcaa cgatttttcg gatttggcaa taacaattca tcatgcatct
     1321 tcacttcaac aagcagctga gctgggaacg gaactcctgg ccaggggaga agctgatttg
     1381 atgatgaaag gcttaatccc gacggcagat tttctccggc cgctgcttaa tacagaaaag
     1441 catttaatta aaaccggaag gatgcttacc catgttgggg ctatttccat accaaatcgg
     1501 ggtcggttga ctttgatcag cgatgccgcg attatgatta accccgatct gagggccaag
     1561 gaaatcatta ttgaaaatgc gatttgtgta gcccgggcct tggggtacaa tccgccgaaa
     1621 gtggcggttc tctccgcttt ggaagctgta aaccccaaaa ttcccagttc tgttgaagcg
     1681 gggaagctta aagaaatgag ccaaagtgga cgttttggcg attgtgtggt ttcaggtccc
     1741 ttagccttgg ataacgcttt atgtcacgac gccgctgtta ctaagggttt aaacggggac
     1801 ccggtggctg gtgaggcgga tgtcttaatc tttcccgaat tagtcagcgg gaatatcctt
     1861 tacaaatctt tcagcatcat cgccggatac cccagcgccg gagtggtcat gggggcgaaa
     1921 gtaccgatta tcttaacttc tagggctgat cccccggagg ctaaggtcaa ttcgattgcg
     1981 ctggcttgtt ttcttgcgca aaacactccc gggtgagatg tcctcacccc tcagcaacat
     2041 ccgggtctcg cgtacacggt acgcgcgccc tcctccataa cgccatc
//
LOCUS       DASVMB010000150         7261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_18982,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000150 DASVMB010000000
VERSION     DASVMB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18982"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VF531_04630"
     CDS             complement(<1..363)
                     /locus_tag="VF531_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7713285.1"
                     /translation="MISNRFVVDASVALSWLLPGEDTNQTLLLRDYAVKNSRVELLVP
                     PIFWYEVANVLWVAAKRERITQTEATQALEALFDFQLDVWVADPVDSLSIAFNQNLAV
                     YDSAYLSTAVQCQATLWTI"
     gene            complement(360..593)
                     /locus_tag="VF531_04635"
     CDS             complement(360..593)
                     /locus_tag="VF531_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEX7713286.1"
                     /translation="MAEKISVYVDKELHRALKTEASLRGKSLSEFMVEAAVRTLHAPD
                     RKMSSSKMDHIRNSVKGTISSEELRVMRNEGRQ"
     gene            complement(728..1225)
                     /locus_tag="VF531_04640"
     CDS             complement(728..1225)
                     /locus_tag="VF531_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713287.1"
                     /translation="MRNILLAIMVFFLFWLGFQSRALAIADINESGASAGVLKTTTII
                     TGTIIAGEATALLLGVHVFAGGDNPWISSKNDLLLILDLITGIGMVYLAASDSGFYDS
                     PIFLVLSNVALLTHGYREWEYLGGSPNSFCANLPLFIVNNLKLVGLVSVSAQSISLKI
                     NFINF"
     gene            complement(1248..1853)
                     /locus_tag="VF531_04645"
     CDS             complement(1248..1853)
                     /locus_tag="VF531_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7713288.1"
                     /translation="MKLREQKRLRCEQEIFAAAMELFRMKGFSQTTMQDIADKAEVAV
                     GTLYNYFDSKGDLLISYLSASSGSIMEKAQVAIEKEHPDPEKAVLELFGIFSELFTSH
                     EKSLWREYLSAAYNDSNKLSGRIWGTQQGFQELLVKLIVDFQKEKIINPNLNPAVAAS
                     VLYSSFFLQFLRYINTDLSLSQLLQDLNAQIYVTFQGLNNH"
     gene            complement(2028..3491)
                     /locus_tag="VF531_04650"
     CDS             complement(2028..3491)
                     /locus_tag="VF531_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7713289.1"
                     /translation="MEIYVVQPGDTLYRIAQRYGTTVAALQSVNQLPNPDRLVIGQAI
                     LIPSPPLEPLRYTVQPGDTLYQLALLFNTTVSQIAQANQIINPNQIAVGTTLLIPGWS
                     QFNYQVQPGDTLSLIAGRYGISVAMIAKVNQIANPALIYPGQILRIPQITPTKPNIEV
                     LAYIMASDISGMEQSLPQIGSLLTYGVVFQFPVDATGAITVPDNTARAVSVLKQYNVQ
                     PSPAVTNWGANGFDSDLARSIIGNDTVKTRTINNILTILNQFGFAGVNIDFENMYPDD
                     RTLFNNFIRDLVAALKPQGYSVSVAVPAKFSDQPSAAWVGTFDYATLGQLADFIFLMT
                     YEWGWIGGPPMAIAPINLVRRALTYATSLIPPAKILQGIPLYGYNWPIPQSPDRPAVP
                     VNLVEVYDLALRYGVNINYDSQSQAPWFRYTDEQGQQHEVWFEDARSITAKYTATRDF
                     NLRGVGYWSYWGNPYGFPQNWVILNDMFRVVKIPAGS"
     gene            3860..4234
                     /locus_tag="VF531_04655"
     CDS             3860..4234
                     /locus_tag="VF531_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713290.1"
                     /translation="MAKILIVDDEPAIRFLISATLEDEGYELFEAADGLEASELIQEN
                     RPDLVILDVMMPGLTGYELCSRIKQDPVTQKIKVIMLTAKAQEQDKLQSRQAGANYYL
                     RKPFSPMELTQVVADLLQKTEQ"
     gene            4255..6342
                     /locus_tag="VF531_04660"
     CDS             4255..6342
                     /locus_tag="VF531_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7713291.1"
                     /translation="MKFRIQTKILIGYILIITAVAGVIIGVHVGSNARIAAARRMDDR
                     YREAENLAAQLMIYTRIGESNIENFLVDGNRQKLIRLKQLQTESQKNLNRLLLLFPGD
                     SQIKTAIQDSMESTVIYFYAVTKAAESGDIRQIKEERAGYQKQLTRSLQSQLNVLNNL
                     SAKHDKAAAAYMALSREAYLITLVLTLLLALAAIIVGLFIARGITFSLNTLMKSIKAV
                     NKGQYQLAAKTNTYDEFEDMIDAFNQMVTTIKNHELSLEEKNTELQAQQESLAAQNEE
                     ILAQQDQIQQAFTDLSKHEELLGRLYRFSQSLTETIELNKLVQNALNGMMKEADAQVG
                     SFLLYDEDSSELTSKMSMGLIDSGSVKFQLNEGLVGRTAAERKSLIARFNEGQLQSEG
                     IRGKLTMASEIYLPLTSDNQLLGVIALGRSGKNDFSEEEQKRLISLADQIAVALHNSL
                     AHLEIKQSLKRVQELDKLKSELINTVSHELRTPLASIYGFAELLLKKPPEATKAKKYL
                     TTIYHESQRLGELINNFLDLQRIEAGRFEVTKQPVHLTELIQSNIQIYQGQSPIHTLA
                     AKIEPNLPQVLTDPDRVSQVLGNLLSNALKYSPAGGLIEVRAFKRSAAEVAIAVYDHG
                     LGIPKEALANIFHPFFRVDNSDRRQIGGTGLGLAICQRIMHTLGGTIEVQSEHGRGSI
                     FTFTIPISGYTGS"
     gene            6536..>7261
                     /locus_tag="VF531_04665"
     CDS             6536..>7261
                     /locus_tag="VF531_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713292.1"
                     /translation="MKENPAQNKLEKAKAIFRQEVQQRLPECQEALSFLKSNGYDAQA
                     CETIYRFAHTLKGSGQMVGWWDIADPAAEMATALILLKNYGVEFSTGILNFLNERLEE
                     ILNEMNGSSEITSSIDPFTQTASTPKILIVDDDPTITHLVAESLEQNGFEAIVCHDLV
                     TAEQQLRLVQPDLIVLDIILPQESGIEFCRKIRSDPQWRIVPVIFLSVKDQLQDKLAG
                     FSTGADDYICKPFKVEELIARIRA"
BASE COUNT         2018 a         1739 c         1637 g         1867 t
ORIGIN      
        1 tatcgtccat aaagttgcct gacactggac cgctgtactc aagtaagcgg aatcatatac
       61 ggcaagattc tgattaaaag caattgaaag actgtcgacc gggtccgcca cccaaacatc
      121 aagctgaaaa tcgaaaagag cttcaagtgc ttgcgtggct tctgtttggg ttattcgttc
      181 cctctttgcc gctacccaaa ggacatttgc tacttcatac caaaaaattg gtggaacaag
      241 caattcaacg cggctgtttt ttacggcata gtcgcgcaag agcaaagtct ggttggtatc
      301 ctctcccggc agaagccatg acaaagctac cgacgcatca acaacaaaac ggttactgat
      361 cactgcctgc cctcattccg cataactctg agctcttctg acgagatagt cccttttacg
      421 gaattacgaa tatgatccat tttggacgaa gacatcttcc ggtccggagc atgaagtgta
      481 cgcactgccg cttcaaccat gaattctgaa agggacttgc cccgtaaact cgcctctgtc
      541 ttaagagcgc gatgcagttc cttatccaca tacacactga ttttctccgc catttcatcg
      601 cctccttgtt ttttcattat agctctttaa cgctatagag tcaataaaat ccattccagt
      661 ttatggatca tacttaatgt cgccggctca atctatccct atccactatc tcatgtaggc
      721 tgccttttta aaaattaata aaattgatct tcaacgaaat actttgggct gagacagaaa
      781 ctaacccaac cagtttgaga ttgttgacga taaataacgg tagattggca cagaaagaat
      841 tggggcttcc cccaagatac tcccattccc ggtaaccatg agtgagcaaa gcgacattcg
      901 atagcaccag aaagattggg gagtcataaa agccggagtc gcttgctgcc aaatagacca
      961 tgccaatccc cgtgatgaga tccaatatta aaagaagatc atttttcgac gagatccacg
     1021 gattgtcgcc gccggcaaag acatgcaccc ccagcaataa tgcggtcgct tccccggcaa
     1081 tgatggtacc tgtaataata gtggttgtct ttaaaactcc ggcggaagcc cccgattcgt
     1141 tgatgtcggc tattgcgagc gccctgcttt gaaaacccaa ccaaaacaaa aagaatacca
     1201 ttatggctag tagtatattt ctcatcgtta ccacactccg caacggttta atgattattt
     1261 aacccttgaa aggtcacgta gatttgagca ttgagatctt gcagtaattg ggatagggac
     1321 aaatccgtat tgatgtaccg cagaaattgg aggaaaaaag agctgtataa aaccgacgcc
     1381 gcaaccgccg ggttcagatt ggggttgatg attttctcct tttggaaatc aactatcaac
     1441 ttaaccagca attcctgaaa tccttgttga gtcccccaga tgcgcccgct taacttgttt
     1501 gaatcgttat atgccgcaga tagatactcc ctccatagac tcttttcgtg ggaagtaaac
     1561 agttccgaga aaatcccgaa taactccaaa acagcctttt ccggatctgg atgttccttt
     1621 tctattgcta cctgagcttt ttccataatg gaaccgctgg atgccgacag ataggagata
     1681 agcaagtccc ccttggaatc aaaataattg tatagagtgc ctacggctac ttcggcctta
     1741 tcggcaatat cttgcatggt tgtttgacta aaccctttca ttctgaataa ttccatggcc
     1801 gccgcaaaaa tctcttgttc gcaccggagt cgtttctgct ctctcagttt cacggaagac
     1861 acccctttat ataaaataaa atgaatgcat tcattattat aatgcgttca ttttatttgt
     1921 caatccattc ctttttaaag tcaccaaaaa tgaacgaatt cccggaaatc cgggggattc
     1981 tgccgacgtc attcggcttt tggaaaatta caccggctgc tcactgctta actccctgcc
     2041 gggatcttta ccacacgaaa catatcgttt agaattaccc agttttgcgg gaagccataa
     2101 ggatttcccc aataactcca gtacccgaca ccccgtaagt tgaaatccct ggtcgcggta
     2161 tatttcgccg tgatcgatcg cgcgtcttca aaccacactt catgctgttg cccctgttca
     2221 tcggtatacc ggaaccaggg cgcttgcgat tgagaatcat aattgatatt caccccgtac
     2281 cggagcgcga gatcataaac ttcaaccaag tttacgggaa ccgccggccg gtcgggagat
     2341 tgagggatcg gccaattata cccgtatagc gggatcccct gaagtatttt ggctggtgga
     2401 atcagtgagg tggcatatgt cagagcccgc cgcaccagat tgatgggggc gatggccatg
     2461 ggcggtcccc cgatccatcc ccactcgtag gtcattagga aaatgaaatc cgccaactgc
     2521 cccagcgtag cataatcgaa agttcctacc caggctgcgc tgggctggtc ggagaacttt
     2581 gccggcaccg ccaccgatac cgaatatcct tggggtttca aagctgccac cagatcccgg
     2641 ataaaattgt taaagagagt ccgatcgtca ggatacatgt tttcaaagtc gatatttact
     2701 ccggcaaaac cgaattgatt gagaatagtc aatatattat tgatcgtcct ggttttgacc
     2761 gtatcattac cgatgataga tcgagccaaa tcggaatcaa agccgttggc tccccagtta
     2821 gtaactgcag gggacggctg gacattatat tgcttgagaa cacttaccgc ccgggctgta
     2881 ttgtccggca cggtaatggc tcccgtggcg tcgaccggga actggaacac aacgccgtat
     2941 gtcagcaaag acccgatctg gggcagtgac tgttccatcc cggatatatc gctggccatg
     3001 atataagcca gcacttcaat attgggttta gtgggagtga tttgtgggat gcgcaggatt
     3061 tgccctggat aaatcagcgc cgggttcgca atttgattga ccttggcaat catggctacc
     3121 gaaatgccgt atctcccggc gatcagggat agcgtatcac cgggttgaac ctggtagtta
     3181 aactgtgacc agccgggaat gagaagggtc gttccaacag caatctggtt gggattgatg
     3241 atctgattgg cttgggcaat ctgactaacc gtcgtattga agagtagcgc cagttggtaa
     3301 agggtatcgc cgggttgaac cgtgtaacgc agcggctcta gcggtggact gggaatcaat
     3361 atcgcctgcc cgatcaccag ccggtccggg ttgggcagtt ggttgacgct ttgaagcgcg
     3421 gccacggttg ttccgtacct ttgggctatc cggtaaagcg tatcaccggg ttgaaccaca
     3481 taaatctcca tgagatcact tcctaaataa aatctgggat gtgattagca tatgtgcgga
     3541 aatcccggag agttacggat aaccattatc agatcaaaaa agttgccctt ccactatcta
     3601 tattggttat caagacgcat aaacaatgaa aatagaaaaa acgatgacct taccgttttt
     3661 tactaatcca ggaaaatatc aaaaataata acataagtct ttttttaggt tgctaaataa
     3721 attaccttga gttataaatt aaaattgccg atgaaaatat tgttctatac ttttttgtag
     3781 tttatagtca gaatttttaa atgtattcat aaaatatttc ttcctcgtgt ttaaatacta
     3841 ctgtaaattg aggtgcacta tggctaagat tctcattgtc gacgatgaac ccgcgattcg
     3901 ttttcttatt agcgccacgc tggaagacga ggggtacgaa ctttttgagg cagccgacgg
     3961 cctggaggcc tccgagctta ttcaagaaaa tagaccggat ttagtcatcc ttgatgtgat
     4021 gatgcccggt cttaccggat atgaactttg cagccgcata aaacaggatc ctgtcaccca
     4081 aaaaataaag gttatcatgc tgacagccaa agcccaggag caagacaagc tccaaagccg
     4141 gcaagccggg gcgaattact atttacgcaa accctttagc cctatggaat taacccaagt
     4201 ggtcgccgat ctgctgcaaa aaacggaaca ataagttttc acggaggttt accaatgaag
     4261 tttcgcatcc aaaccaagat cctcatcggt tatattttga tcatcaccgc cgtggcgggc
     4321 gtgattatcg gagttcatgt cggctccaat gcccgaattg ccgccgcacg gcggatggac
     4381 gaccgttacc gtgaagctga gaacttagcc gctcagctta tgatctatac ccggattggg
     4441 gaatcaaata tcgaaaattt tcttgttgat ggaaaccgtc aaaaattaat cagattgaaa
     4501 cagcttcaaa ccgaatccca aaaaaattta aaccgcctgc tgctgttatt ccccggagat
     4561 tcccaaatca agaccgccat tcaagactct atggaatcaa cagtaattta tttttatgct
     4621 gttactaaag ccgccgagag cggtgatatt cggcaaataa aggaagaacg cgcgggatac
     4681 caaaagcaat tgacccggtc gctccaatct cagcttaatg tcctcaataa tctcagcgca
     4741 aaacatgata aagcggctgc tgcgtatatg gctttatccc gggaggctta cctgattaca
     4801 ttggtattaa ctcttctcct ggccctggcc gctatcatcg tcggattatt catcgcccgc
     4861 ggcattactt tttccctcaa tactttgatg aaaagcatca aggctgtcaa taagggtcaa
     4921 tatcaactcg cagctaaaac aaatacctat gatgaatttg aggatatgat cgatgccttc
     4981 aatcaaatgg tgaccacaat caaaaaccat gagctttcat tggaggagaa aaataccgag
     5041 ttgcaggcgc aacaagaatc ccttgctgcg caaaacgaag agatccttgc gcaacaggac
     5101 cagattcaac aggcctttac cgatctctcc aaacacgagg aattgttagg ccgtctttat
     5161 cgctttagcc aaagcctgac tgagacaatc gagctgaata agttggtaca aaacgctctt
     5221 aacgggatga tgaaagaagc cgatgcccag gtgggttcct ttttactcta tgatgaagat
     5281 agctctgaat tgacttccaa gatgagtatg ggtttgatcg actccggttc cgttaaattt
     5341 caacttaatg agggtttggt cggacgtacg gccgcggaac gaaaaagcct gatagcaaga
     5401 tttaatgaag gccagttgca gtccgagggg atacggggaa aactgaccat ggcgagtgag
     5461 atttatctgc cgttaacatc cgacaaccaa ctgcttggag ttatcgcttt gggacgttcc
     5521 ggaaaaaacg atttttcaga ggaagaacag aaacgcttga tatcgctggc ggatcagatc
     5581 gccgttgctt tacataactc cctggcccat ttggaaataa agcagtcttt aaaacgcgtt
     5641 caagaactgg acaaattgaa atcggaactg attaacacgg ttagccacga attgcggact
     5701 ccgttggcca gcatctatgg atttgccgag ttgctgctta aaaaaccccc tgaagctaca
     5761 aaggccaaaa agtatttaac aaccatttat catgaatctc aacgcctcgg tgagctcatc
     5821 aataactttc tggatttgca aagaattgaa gccggacgat ttgaggtaac taaacaaccg
     5881 gttcatttaa ccgaattgat tcaaagcaat attcagattt atcagggtca aagccccatc
     5941 cataccctgg cggcaaaaat tgaaccgaat ctgccgcaag tcttaactga tccggatcgg
     6001 gtctcccaag ttttggggaa ccttctatcc aacgccctca aatattcccc tgccggcggg
     6061 ctcattgagg ttagagcctt caaacgctcc gccgccgagg tcgcgattgc cgtatacgac
     6121 catggtttgg gtattcctaa agaggctttg gccaatatct ttcatccttt tttccgggta
     6181 gacaattccg atcgccgcca aatcggaggt accggtttgg gactggcgat ttgccagcgc
     6241 atcatgcaca cactgggcgg gaccatcgag gttcaatcgg agcatggccg gggcagtata
     6301 tttactttta ctatcccgat ctccggatac actggttcat agtattcgta ctgtcacccg
     6361 ttttacggac ggagtcctcg gtctgccgga tccgggacac cttccggaac ttgagggttc
     6421 gttgaagaga ttgttgagcg ctaacggttt tgctgtcaaa tcaattacaa aggaacccga
     6481 tttgctggtg atcaggattg aaaacccact catcactaaa aaaggagtga gctaaatgaa
     6541 agaaaatccc gcccaaaaca aacttgagaa agccaaggcc atcttcaggc aagaggtcca
     6601 acaacgcttg cccgaatgtc aggaagcgct gtcgtttctt aaatcaaacg gttatgacgc
     6661 gcaggcctgc gaaacaatat atcgctttgc ccataccctc aaaggttcgg gtcaaatggt
     6721 cggctggtgg gatattgccg accccgccgc cgagatggcg acggccttga ttctattaaa
     6781 aaactacgga gtggagttta gcaccggtat cctgaacttc ctcaacgaac ggctggagga
     6841 gattctcaat gaaatgaacg ggagttctga aataactagc tccattgacc cgtttacgca
     6901 gacagcctcc accccgaaaa ttttaattgt ggatgacgat ccgaccatca cccatttggt
     6961 tgccgaatcc ctggaacaga acggttttga ggccattgtc tgtcatgact tggttactgc
     7021 ggaacaacaa ctgagattag tccaacccga cctcatcgta ctcgacatca ttttgcctca
     7081 agagagcgga attgagtttt gccgcaaaat acgatccgat ccgcaatggc ggatcgttcc
     7141 ggtcattttt ttaagtgtca aagaccaatt gcaggataag ttagccgggt tttcaaccgg
     7201 agccgacgat tatatttgca aaccatttaa agtcgaggag ctaatcgccc ggatccgggc
     7261 a
//
LOCUS       DASVMB010000151         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_95191,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000151 DASVMB010000000
VERSION     DASVMB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95191"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..739)
                     /locus_tag="VF531_04670"
     CDS             complement(254..739)
                     /locus_tag="VF531_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX7713293.1"
                     /translation="MKAKLVVILAVLALASFSFTALAGGRGQVGRMGMGGRMGMGGGL
                     NGLNLSADQQQSILTISQEYQKDTLTLRQDLQKKRLEMQQLWQDTTLKQSALEAKSKE
                     VIALQIQLVTKSRVMQEKIKKVLTPEQLKQWESRGGGKGAGLGGPGLQGGFSGFAGMG
                     I"
     gene            1045..1743
                     /locus_tag="VF531_04675"
     CDS             1045..1743
                     /locus_tag="VF531_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713294.1"
                     /translation="MARIGIIEDELKIQQIVKAYLEKEGFEVCTATDGRQGLELLKTE
                     KLDLVILDLMLPVIPGEQLLTYLRQTSEIPVIILSAKTSEDERIFGLNIGADDYLIKP
                     FSPRELVARVYAHLRRARAGATPSGTVLSFNHRELLIDISKHVVLIQDHPVTLTPTEF
                     RILQVLAQTPGLVYSRSQLVEKVQGYSYAGYDRTIDSHIKNLRQKIESNPETPGYILT
                     VFGVGYKFGGVLDE"
     gene            1736..3142
                     /locus_tag="VF531_04680"
     CDS             1736..3142
                     /locus_tag="VF531_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7713295.1"
                     /translation="MSKSPILRKITLTILAVSLGGVLILGLVLHLTINRQFQTYLDRN
                     ELARQQQVTQALAQFYQENGGWPDYPMMVRFNPMSNLEYVTDRQQRMVMIGGRGRRMN
                     PGAGQGRGADRLVARPVIVNGTRVGTAYFGTNQIQALFSRQDQLFRSAINDSILWSIL
                     IMAVISIGVAVNFAKRLSRPITAMNQIAGDMAAGNLDTRISNLSRDELGELGKSLNRL
                     AERLREGNELRKKMTADVAHDLRTPLATIKSHLEGMIDEVIPTSRENLESLHEEVDRL
                     TLLVNDLQTIAAADVTIQRMTLALLKLDSFLKDLVTRMIPLFRTKGIVLELESMPPLQ
                     LATNAEALTKILDNLLTNAYKFTPAGKNVTLSATKNEKAVAIAVSDQGIGIAPADLPY
                     IFERFYRADPSRNRENGGFGLGLTIARDLAKALGGTITASSSPGQGSVFTVTLPLIPK
                     QDAPSNDDPVSSGEYHPA"
BASE COUNT          793 a          847 c          802 g          799 t
ORIGIN      
        1 tagacttaaa ccgggaaggg ttctggatga ttcgtagtcc ccgccggccc aaacgatcaa
       61 tcgtttctcc gggcaacaac tctatgccgc tctccatcag cgcatccccc caataatctt
      121 ctgtcatcca tggtagcatg aataagggat gggtgcaaga tacggtgaag gggatgattc
      181 gaaagtgatg attcgaatca tcactttcga gtatccttct cgatcatcct tttcgatcgc
      241 cctcaggata gatctagatc cccattccgg caaatcccga gaatccacct tgcagcccgg
      301 ggccacccaa gccggcaccc ttgccgcctc cgcggctttc ccactgcttc aactgttccg
      361 gggtgagcac cttctttatc ttttcctgca tgacccggga tttggtgacc aattgaatct
      421 gaagcgcaat gacctccttg gacttggcct caagagccga ttgcttcaag gtcgtatcct
      481 gccatagttg ctgcatttcc aatctttttt tctggagatc ctggcgaaga gtgagcgtat
      541 ccttctgata ttcctggctg atggttaaaa tggactgctg ttgatcggcg ctcaggttga
      601 gaccgttcaa cccgccgccc atgcccatcc ggccacccat acccatccga ccgacctgac
      661 cgcgtccgcc tgctaaagcc gtaaagctga aactggctag tgctaaaaca gccaaaataa
      721 ccactagttt tgctttcatt tcttttttct cctttctaat tcccatagat attcggacac
      781 tttattctta accccgataa gaaatcatcc gcacggcacc acctcacaat ccgattataa
      841 aaccggtcca tggaagactc atgcggaact tgtgaagatt ttgtggagat tcggccaagc
      901 ctctggattt cttatcacaa aatggttatt cttaagttac aatacgagag ttcatgccac
      961 gcctttatct ggcattcttc tttgaagttc cctgccggat tcggtcgaaa cgttaaactg
     1021 tcttcatcac ctgggggtaa tcggatggcc aggatcggaa ttattgagga tgagttgaag
     1081 attcaacaga ttgtcaaggc ttacctggaa aaggaaggct ttgaggtttg taccgcgacc
     1141 gatggccggc aggggttgga actcctgaaa accgagaaac tggatttggt gatcctggac
     1201 ctgatgctac cggtgattcc gggggaacag ttattaactt acctgagaca aacctcggag
     1261 ataccagtga tcattctctc cgccaaaacc agcgaggacg aacggatctt cgggctaaac
     1321 atcggggctg atgactatct gatcaaacca ttcagcccca gggaacttgt ggccagagta
     1381 tacgcccatt tgcgccgggc ccgggccggg gccaccccgt ccggtacggt tctatcgttc
     1441 aatcaccggg aattactgat agatatcagc aaacatgtgg ttctgatcca ggaccatccg
     1501 gtcaccctga ctccaaccga atttcggatc ctgcaggttt tagcgcaaac ccccgggctg
     1561 gtctacagcc ggagccagct ggtggagaag gttcaaggat acagttacgc gggatacgac
     1621 cggaccatcg acagtcatat taaaaaccta cgccaaaaga tcgaatcaaa tccggaaacc
     1681 cccggctata ttctgacggt tttcggagtc ggttataaat ttggaggggt gctggatgag
     1741 taaatccccg attctgagga aaatcacatt aacgatcctt gccgtatcgt tagggggagt
     1801 cctcattctg ggcctggtgc ttcatttgac aatcaaccgc caatttcaga cctatctcga
     1861 ccgaaatgag ctcgcccgcc agcagcaggt cacccaagcc ctggcccaat tttatcagga
     1921 aaatggtggc tggccggact acccgatgat ggtccggttt aatcccatga gtaatctgga
     1981 atatgtcacg gacaggcagc aacggatggt tatgattggc ggacggggaa gacgaatgaa
     2041 tcccggagcc ggtcaaggcc ggggtgccga tcgtttagtg gctcggccgg tgatagttaa
     2101 cggaaccagg gtgggtacag catactttgg tacaaaccag attcaagcat tgttctccag
     2161 acaggatcaa ttgtttcggt ccgcaattaa tgactctatt ctctggtcga ttctgataat
     2221 ggctgtgatc tccatcgggg tggcggtaaa cttcgcgaaa cgtctttcca ggccaattac
     2281 ggccatgaat cagattgctg gggatatggc cgccggaaat ctggataccc gaatctcgaa
     2341 tctatcccgg gatgaactcg gcgaactggg aaaaagcctg aatcgattgg ctgaaagact
     2401 ccgggaagga aatgaactgc gaaaaaagat gaccgccgat gtggcccacg acctccggac
     2461 cccgcttgcc accattaaga gtcatctgga ggggatgatt gatgaggtga ttcctacctc
     2521 tcgggaaaac ttggaatccc ttcacgaaga ggtcgatcgg ctgactttac tcgtcaacga
     2581 ccttcagaca atcgcggccg cagacgtcac gattcaacgg atgacattgg cccttttgaa
     2641 actggattca ttcctgaaag atctggtcac gaggatgatt ccgctttttc gcaccaaagg
     2701 gatcgttctg gagcttgagt ctatgccgcc gttgcaactt gccactaacg ccgaggcctt
     2761 aacgaagatc ctggataacc tgttgaccaa tgcctataaa tttacccctg ccggaaagaa
     2821 tgtaacctta tccgcaacga aaaatgaaaa agcggtggca attgccgtca gtgatcaagg
     2881 catcggcatt gcccccgctg atttacctta tatatttgag cgtttttacc gggccgaccc
     2941 ttccaggaac cgcgagaacg gtgggttcgg tttgggattg accatcgccc gcgatctggc
     3001 gaaggctttg ggcggtacaa tcactgccag cagcagtccc ggacagggaa gcgtcttcac
     3061 cgtaacatta ccgctcattc cgaagcaaga cgcgccttct aatgatgatc ctgttagctc
     3121 tggcgaatat catccagctt aattaacatt gccacttcgt cacagttggg gaatttggga
     3181 cagttgatgt attccttcca gactttatgg ggcatttctt ccttggttac ctcggtgaaa
     3241 c
//
LOCUS       DASVMB010000152         9486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_19599,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000152 DASVMB010000000
VERSION     DASVMB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19599"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF531_04685"
     CDS             <1..188
                     /locus_tag="VF531_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713296.1"
                     /translation="FDPGKSPQQIKGTIFLTSANSGKRDWIAEYRARAEQALKLGDTQ
                     SYSQYKHIGEILERLSH"
     gene            complement(261..1124)
                     /locus_tag="VF531_04690"
     CDS             complement(261..1124)
                     /locus_tag="VF531_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX7713297.1"
                     /translation="MSQKYLLVSLLIFVIFIVLFGIYMSQNLNREYKRSVRQGFELNF
                     GEKKGLLTLDDIRHLPNPVQKYLIYVGAVGKEKVQNVKLIAAGEMKMNLKSGWSKINF
                     EQYNFFASHLTRLFYIKLNMLGIPILGLHSYTDANASMLVKLGGLIPVVDVKGREMRI
                     SDTVTLLNDMSIFAPAALIDNRIRWESIDDHTAKAIFNPGYCTVSAVLYFNDRGELVN
                     FISDDRYSLEEDGSFKKIRWSTPIRDYQERNGIKLASYAEAVWNRPEGDDSYFRLKNI
                     RQIRLNCDMAQ"
     gene            complement(1155..1622)
                     /locus_tag="VF531_04695"
     CDS             complement(1155..1622)
                     /locus_tag="VF531_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7713298.1"
                     /translation="MMKGSKPANLFEKRALIFANLFLIANKLQVISDQYLTPSGLTAK
                     QWFLIAAIQQFDTPPTLSEVSELSGSSRQNVKQLAIKLEKNDFLRIEKDAQDTRAVRL
                     ILTEKCRSFWERREMQDTQFITELFSCLGETETGILSESVQKILKRVEEIVIH"
     gene            1935..5852
                     /locus_tag="VF531_04700"
     CDS             1935..5852
                     /locus_tag="VF531_04700"
                     /inference="COORDINATES: protein motif:HMM:NF036773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HEX7713299.1"
                     /translation="MDDLQKVSIRQVKQTPWLIEEQGILKSETWLEIENDGAAFEGWV
                     RITVPHRPPYMEHLSTVGCGMSRKKIHVAELQNNGDPVEFSIFENPQGLGEAVCAVVI
                     AQMKIKHWKIYVAHDMHTDLGYTNYQEDLIHKIYPGYLERVFQYVEDTKGWPEDVQFR
                     YPIEASFMLHGSALIAKDADWIMRLKEYLKAGRITYPFDYLHMATEGMGTEEIARQCY
                     ISGRYLQDRLGTGPVKVFVHTDDAGFSWSHVDLLVNAGVKYLSLRLQDANWNYSDGHC
                     PKYPRLFYLKGRNPGSRLLVFDGPPYHFDDFGFRQSNSEETFRLIAEKLPERQGEDYP
                     CDAYLIHLTQVRDNSGVDPNVMKNIRALSEATDENGNKYLYPRFINSVVEDFFEYIEA
                     HFSQDIPAFQGTLESFWSFGAPQVAHETALSRVNHEKVPAAELYSTLACVYTSAAQYP
                     YQEILRAYDNMLLTDEHTWACWKYYAADDQFMWMRNKAIQAERLADQLLEEAFSEIQG
                     LIPSDGKTIVIYNPSVWRRTDLVKVGKENFPEHFEIIDLESGASVGYQWLPDGKAAFI
                     APEVPGLGYKCFRINPRKDDPVFGSDILTTGNSIENKYFRVTLDSTGSISSIIDKENG
                     YREMVDQASPYKMNQFVYYTSRNELYRNDLFSEDSIRQASISAASGCVMGILAAEGTT
                     RGADRIKRNIVLYQSIPRIDIINEVVKSDAPPDYTECAEEAFFVFPFAVSDFVIEHET
                     PNGEIRPSVNPDIHQPMEQLYWSNTDFYAVNRWIDISDRRDYGITFSPVHAPLVQYGG
                     RRTCRWDKDYDTPKPWVYSWIIDNFWKTNFPYTQPGPLTFKYSIRSHGKPDWRTGRAD
                     QFGHEVTNELKCSVLDTIQDGPLDAVRGSFINIDRDNVFLTAAKPAEANGEGIIFRFH
                     ETLGEDSSVTIGFSASFTPEEVWETDIVENDVKSLQVADHRISFFIQGHGWMTIRVKQ
                     GSVPVEITGVKATTDLGGTLVSWDEPVNPGIRYYEVFRDTTAGFMPGTGNYVASVTGG
                     CYYDGQVTKGLSHDYYYRIRAAGTGRKGEASPGVKALPGEIGDTEPPSIPARLRAEAF
                     GSNRVSLSWDGSEDKLTGVRGYRIYRNGAEIKDVTGTITSFLDVGLHANTTCEYWVRA
                     YDTVGNISFPSEPVQVTTPDGQMNGNIAGEAVVTASSELGGAFRATNVADGIFGLMGI
                     GEWVSRNEKQPWVQLNWSETRWIETVYIFGSYGDDHRYSGVLYFDDGILLPLESIRTD
                     GSATKVTLGAKPVRWIRVQVNDEQGAGGLSEIQVIEEQSVNRG"
     gene            5991..6917
                     /locus_tag="VF531_04705"
     CDS             5991..6917
                     /locus_tag="VF531_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713300.1"
                     /translation="MKELTREHKLLKTILLYRYMYLFILPAFIWYFVFQYIPMYGLTL
                     GFKEFRFDMSLWNSPWVGTRYLKEFLTDSMFRPLLFNTVKISLLKLVVGFLAPIVLAL
                     MLNEVISPRFKRVMQTVSYLPYFISWVVVIAIFNKFVSPTDGMINDLKVAAFGGDPIF
                     FMGEAGWFYPIMLLSFVWKNIGWNSIIYLATIAGIDPQLYEAAYIDGAGKWKSMIHIT
                     LPGIVPTAAILLLLSMGSIMYAGYEQIILLKTPGNSLLSQILDTQILEQGIRQGRYGY
                     ATVAGLFQGTIGLILVTISNYVAKKSSGISLW"
     gene            6936..7835
                     /locus_tag="VF531_04710"
     CDS             6936..7835
                     /locus_tag="VF531_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713301.1"
                     /translation="MAFKQSAGEKIFERLNHIILLIFGLSTVYPFVYIITLSFNDGLD
                     ALKGGIYLWPRVFTLDNYIKCFQNELIFNSYMITVFRTIVGTFLATLLCSMLAFALTK
                     KKLPGRSAILFFFFFTTIFSGGLIPYYILLRQLNLTNSIWVYVIPQLYSFYNVIILKT
                     YFETIPKSISESAVIDGCSDVQIFSKIYLPLSGPVIATILLFFGVAHWNDWFTGTYFV
                     QDKRLFPAATMLQNLLTESTYESSTFDTTKMNTSLAMSGVTTTPESLKMAFIVIMTLP
                     IVFVYPFLQKYFIKGVMIGAIKG"
     gene            7909..>9486
                     /locus_tag="VF531_04715"
     CDS             7909..>9486
                     /locus_tag="VF531_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713302.1"
                     /translation="MKRSPKVAIYVTLVVFLVISLLTASAAQSATRKQAKGVKLTFLM
                     DWNGTASSKGMPDSINNPVARKIKEKTGITVDIEYATTNEAERLNLMFASGNMPDIVN
                     APFWGGSNAETIAIKKAATEGLLLNLDPLIAKYGPNLKDAFQKGLAIGYKENDINDPA
                     FGGKHFVLPQQAPATKDDVLNWGGGLYCRKDILDKLKINPASINSSEALYNLLKAIKK
                     GEFKDINGKAVIPAGAWGDGWDYVEFWRPFRQDRRAEFDYIDGKFVYRANNPLLDQRV
                     LFMRKLITEGLFDPECLRQSDSQAKEKMATGRVAMVGCHYFHIRDFMNNTLYKTNPQM
                     HYITIGPLPWSDGITTVVEVPDRNGTPVIFLPKTCADPVAAIRFLNYLNSDEGQLLAY
                     YGIKGTHYTMVNGKPRMAKKWLDIWKSDPKKLQALGIRSTFMDMICLDKRLSAYGELL
                     PGEADNPDPWYQQAKSVRRIEFKSGHTINDITNQYPRLDAVRSLVDWNLYRDSTERAY
                     FAKSDEEALKILNDFREQ"
BASE COUNT         2497 a         2164 c         2328 g         2497 t
ORIGIN      
        1 cttttgatcc cggcaaatcc ccgcaacaga taaaaggtac cattttctta acctcggcta
       61 actccgggaa acgcgattgg attgccgaat atcgggcgag agccgaacaa gcattgaagt
      121 tgggcgatac acaaagttat agtcaatata aacatattgg cgagatttta gaacgtttgt
      181 cacattaaat atggataaaa atttatttgg cgtttgtcct tttgtgacgg cgctgaaaat
      241 actcaaaggc aagtgcatac ttattgcgcc atatcacaat tcaaacgaat ttgccgtata
      301 ttttttaatc gaaagtagct atcgtcaccc tccggcctat tccatacagc ctccgcatat
      361 gaagcgagct tgattccgtt tctttcctga taatctcgta ttggagtcga ccagcggatt
      421 ttcttgaaag agccgtcctc ctccaaggag tatcggtcgt cactgatgaa gttgaccaat
      481 tctccccgat cattaaagta taataccgcg gaaaccgtgc aataaccggg attgaaaatc
      541 gcttttgcag tatggtcatc gatggactcc cagcggatcc ggttatcaat cagagccgcc
      601 ggcgcaaaga tggacatgtc gttaagcagg gtgactgtgt cactgattct catttcccgg
      661 cccttcacat cgactaccgg aatcaacccc ccgagcttga ctagcatact tgcattcgca
      721 tcagtgtaag agtgcaaacc caggatcgga atacccaaca tattcaattt gatataaaac
      781 aaccgggtga ggtgactggc gaagaagtta tattgctcaa aattaatttt tgaccagcct
      841 gatttgagat tcattttcat ttccccggcg gcaatcaatt ttacattttg gaccttttcc
      901 ttgccaacgg caccaacata gatgagatat ttttgaaccg gattcggtaa atggcggata
      961 tcatcgagcg tcaatagacc ttttttctct ccaaaattca gttcaaaccc ttgtcttacg
     1021 gaccttttat attcccggtt tagattttga gacatataaa tcccaaatag cacaatgaat
     1081 atcacaaaaa tcaataagga tacgagcaga tatttttggc tcatttcaaa tcccccagtt
     1141 ccataatggt gaagtcaatg aattacaatt tcctcaacac gtttaaggat tttctgtacg
     1201 ctttccgaca ggatgccggt ttccgtttcg ccgagacagc taaatagctc cgtgataaac
     1261 tgagtatctt gcatttcccg ccgttcccaa aatgagcggc atttttcggt caatatcagt
     1321 cgaactgccc tggtatcctg tgcatctttt tcaatccgta aaaaatcatt tttttcaagt
     1381 ttgatggcca actgtttgac attctggcgg gaactgccgc taagctctga tacctcgctg
     1441 agagtggggg gagtatcaaa ttgctggatg gccgctatca aaaaccattg ttttgcggtc
     1501 aatcccgatg gtgtaagata ttggtcgctg atgacctgta gtttattggc tatcagaaag
     1561 agattagcaa aaatcaatgc ccgtttctca aagaggttgg ccggttttga acctttcatc
     1621 atctatcctc catcaaatac gtaacacgtt acccttatag gtaatatatt acgtatttga
     1681 tcatttgtca atattcttgt tgatattttc cctatcatcc ggagaggccg atcccggtac
     1741 gaaaagtaag tgcggcacag actatctggg attccatgat gtgagaatcg tccactgacc
     1801 ttccgagttt cgtccgttta caaggagctt tccatttcgg tatgatgacg ttatatcaga
     1861 taacgctata tcaatagtct atttgagggc agagagttaa ccgttccaat atttttttag
     1921 cggaggaatc ggatatggat gatttgcaga aggtttccat ccgacaggta aagcagacac
     1981 catggctcat tgaggaacag ggcatcttaa aatctgaaac ctggctggaa attgagaatg
     2041 atggcgccgc ttttgaaggc tgggtcagaa ttacggttcc tcatcgtccc ccttatatgg
     2101 aacacctgag tacggtcggt tgcggaatga gccggaaaaa gattcatgtg gcggaattgc
     2161 aaaacaacgg cgatccggtt gaattctcca tattcgaaaa tccgcaaggc ctgggtgaag
     2221 cggtatgtgc cgtagtgatt gcgcaaatga agatcaaaca ttggaagatt tatgttgctc
     2281 acgacatgca taccgatctg ggatatacca attatcagga ggatttgatc cataaaatct
     2341 atccgggata tctggagcgg gtgtttcaat atgttgaaga taccaaaggc tggccggaag
     2401 acgttcaatt caggtatccc attgaagcat cctttatgtt acacggttcc gcgttgatag
     2461 cgaaggatgc cgattggatc atgagactca aagaatatct gaaggccggg aggattacct
     2521 atccctttga ttatctacat atggcgacgg agggtatggg taccgaggag atcgccaggc
     2581 aatgctatat ttccggcagg tatttacagg acaggctcgg aaccgggccg gtgaaagtgt
     2641 tcgtccatac cgacgatgcc ggcttttcct ggtcccatgt ggatctgctg gtaaatgccg
     2701 gagtcaagta cctgtccctc cgtctccagg acgcaaactg gaattacagt gacgggcact
     2761 gccccaaata cccccggctg ttttacctca aggggaggaa ccccggaagt agactattgg
     2821 tatttgacgg tccgccctat catttcgacg attttggatt ccggcagtca aatagcgaag
     2881 agacctttcg gctgattgct gaaaagctcc cggaacgtca gggggaggac tatccgtgcg
     2941 atgcttatct gatccattta acacaggtca gggataattc cggagttgac cccaatgtca
     3001 tgaaaaacat cagggcttta agcgaagcca ccgatgaaaa cggcaacaaa tatttatatc
     3061 ccagatttat caattcggtg gtcgaggatt ttttcgagta tattgaagcc catttcagcc
     3121 aagatatccc ggctttccag ggaaccctcg aaagtttctg gagtttcggt gcaccgcagg
     3181 ttgctcacga aaccgcccta agccgggtaa accatgaaaa agttcccgcc gcggaactct
     3241 attcaacctt agcttgcgtt tatacctccg ctgcccaata cccttaccaa gaaatcctga
     3301 gggcgtatga taatatgctg ctcaccgatg aacacacctg ggcctgctgg aaatattacg
     3361 ccgccgacga tcagtttatg tggatgagaa ataaggccat tcaggcggag agacttgccg
     3421 atcaattgtt ggaagaggca ttctcggaaa tccaggggtt gattccgtcg gatggcaaaa
     3481 cgattgtcat atataatccc agtgtgtgga gacggaccga tctggtcaaa gtgggaaaag
     3541 agaactttcc cgaacatttc gagatcatag atttagaaag cggagcatcg gttggctatc
     3601 aatggctccc cgacggtaag gcggctttta tcgccccgga agtaccggga ttgggttata
     3661 aatgttttcg gatcaatccg cgtaaggatg atccggtttt tggttcggat atccttacca
     3721 ccgggaattc catagaaaat aaatatttcc gggtcaccct cgacagtacc ggatcgattt
     3781 caagcatcat tgataaggag aacgggtacc gggagatggt ggatcaggcc tcgccgtaca
     3841 aaatgaatca gtttgtctat tatacttccc gaaatgagct ttatcgcaac gatctttttt
     3901 ccgaggacag cataagacag gcgtctatct ccgccgcaag cgggtgtgtc atgggaatac
     3961 tggcggcgga gggcacgacc cgaggagccg accggatcaa aaggaatatc gtgttgtacc
     4021 aatccatccc gcggatcgat atcatcaatg aagtcgtcaa gagtgacgcc cctccggatt
     4081 atacggaatg cgcggaagag gcttttttcg tattcccctt tgcggtttcg gactttgtca
     4141 tcgagcatga gaccccgaac ggggagatcc gaccctcggt aaaccccgac atccatcagc
     4201 ctatggagca attgtactgg tccaatacgg atttttacgc ggtcaaccgc tggatcgaca
     4261 tcagtgatcg ccgggactat gggatcacat tctccccggt tcatgctccg ctggtgcagt
     4321 acgggggaag aaggacgtgc cgttgggata aggattatga tacccccaaa ccctgggtct
     4381 acagctggat catcgataat ttctggaaaa cgaatttccc atatacacaa cccggaccgc
     4441 ttaccttcaa atactccatc cgttctcacg gcaagcccga ctggagaacg ggaagggcgg
     4501 accaattcgg tcatgaagtt acgaatgaat taaaatgctc ggtccttgat accatccagg
     4561 acgggccttt ggatgccgtt cgaggcagtt tcatcaacat cgaccgggac aacgtatttt
     4621 taactgccgc caagccggcg gaggccaacg gggaagggat catctttcgc ttccatgaaa
     4681 cccttggtga agattcctct gtcaccatcg gtttttccgc gagttttacc ccggaggaag
     4741 tttgggaaac cgatattgtt gaaaatgatg taaagtcgct tcaagtggcg gatcaccgaa
     4801 tttctttttt cattcaaggg catggctgga tgacgatccg ggtgaaacag ggttctgtac
     4861 ccgtagagat aaccggggtc aaagctacca cggacctggg aggaaccctg gtaagctggg
     4921 atgaacctgt aaacccggga atccgctatt atgaggtgtt cagggataca accgccggtt
     4981 ttatgcccgg aaccggaaac tatgtcgctt ccgtgaccgg tggttgctat tacgatgggc
     5041 aagttaccaa gggacttagc catgattatt attacaggat cagggctgcc ggtacaggca
     5101 gaaaaggaga ggcttctccc ggtgtcaagg cccttccggg ggagattggc gacacggagc
     5161 cgccttctat tccggcgagg ttgagagcgg aggctttcgg aagtaaccgg gtgtcgcttt
     5221 cgtgggatgg ctccgaagat aaactgaccg gggttcgagg gtacagaata taccgtaacg
     5281 gggctgaaat caaggatgtt accggcacca tcaccagctt tctggatgtt ggacttcatg
     5341 cgaacaccac ctgcgaatat tgggtcagag cttatgacac ggtgggtaat atttctttcc
     5401 ccagtgaacc ggtgcaggta acaaccccgg atggtcaaat gaacggtaat attgccggcg
     5461 aggcagttgt aacggcttct tcggaacttg gcggtgcttt tcgtgccact aatgtggcgg
     5521 acggcatctt tggccttatg ggcatcggag aatgggtatc ccgaaatgaa aagcagccat
     5581 gggtacaact gaactggtct gaaacgagat ggatcgaaac cgtctacata ttcggaagct
     5641 acggtgacga tcatcgatat agcggtgtat tgtactttga cgacggaata ttgctgccgc
     5701 ttgagagcat caggactgac ggctcggcga ctaaagtgac cttgggagcg aaacccgtcc
     5761 gttggatccg ggtgcaggtc aatgatgaac agggagcggg aggattatcg gagattcagg
     5821 tgattgagga acaatccgtg aaccgtgggt gaaagatctg agaatcgtcc actcgccctc
     5881 gaagatccat ccgtttaccg tgtcgtgtac ccaaagtata atggatttat gatgtttgaa
     5941 tgaacatcgg agacttgcct tacgactcaa tgaaattgaa ggtgcgggat atgaaggaac
     6001 tgactcggga acacaagctt ttaaaaacaa tcttgctata caggtatatg tatctattca
     6061 ttttgcccgc tttcatctgg tactttgtgt tccagtacat tccgatgtac ggactcactt
     6121 tgggctttaa agaattccgt ttcgatatga gcctttggaa cagtccctgg gtcggaacgc
     6181 gttatctgaa ggagttcctt accgacagta tgttccggcc gttactgttc aataccgtta
     6241 agattagctt attaaagctt gtagtcggtt ttctggctcc catcgtcctg gccctgatgc
     6301 tcaatgaggt gatcagtccc cggtttaaac gggtcatgca aaccgtctcc tatttgccgt
     6361 atttcatctc ctgggtggtg gtgatcgcca tttttaataa attcgtatca cccaccgatg
     6421 gaatgattaa tgatttgaag gttgcagctt tcggtgggga tccgatattc ttcatggggg
     6481 aggccggttg gttttatccc atcatgctgt tgtcgtttgt ctggaagaat atcgggtgga
     6541 attccattat ctatttggct accattgccg gaatcgatcc gcagttatat gaagctgcat
     6601 atatcgatgg cgccgggaaa tggaagagta tgattcatat cactcttccg ggtattgttc
     6661 caactgcggc aatcttgttg ctgctatcca tgggtagtat catgtatgcc ggatatgagc
     6721 agatcattct gctgaaaacc cctggcaata gtttattgtc acaaattctg gatactcaaa
     6781 tcctggagca gggaattcgg cagggaagat acggatatgc tactgtggcg ggactattcc
     6841 agggtacgat cggactgatt ctggtcacca tatccaacta cgttgcgaaa aaatcttcgg
     6901 ggatctcttt atggtaatca aggaggatat ggaacatggc attcaagcaa tccgccggtg
     6961 aaaagatttt tgaaagactg aaccatatta tactcctgat tttcggtctt tcaacggtgt
     7021 atccgtttgt gtatatcatt acgctttcct ttaatgacgg tctggacgcg ttaaaaggcg
     7081 ggatctatct ctggcccagg gtatttacac tggacaatta tatcaagtgc tttcaaaacg
     7141 agcttatttt caattcctat atgattacgg tattccggac catcgtcggc actttcctcg
     7201 ctacactgct ctgctcgatg ctggcatttg cattgaccaa aaagaaactg ccgggccgtt
     7261 ccgccatatt gttcttcttt ttctttacta cgattttcag cgggggactg attccgtatt
     7321 atattctcct caggcagtta aacttgacga acagcatatg ggtttatgtg attcctcaat
     7381 tatacagttt ttataatgtc attattttaa agacatactt cgagactatc cccaaaagca
     7441 tatcggaatc ggctgttatc gacggttgca gcgatgtcca gatattctcc aaaatatatc
     7501 tgccgctatc cggaccggtt atagccacta ttttgctctt tttcggggta gcccactgga
     7561 atgattggtt taccggaact tacttcgttc aggataaaag acttttcccc gccgcgacga
     7621 tgctgcaaaa tttattgacg gaatcgacct atgaaagttc aacctttgat acgaccaaaa
     7681 tgaatacttc gctggcgatg tccggggtta ctaccacacc cgagtcgctt aagatggctt
     7741 ttattgtcat catgaccctt cccatcgtgt ttgtgtatcc ttttcttcaa aaatacttta
     7801 taaaaggagt gatgattggg gcgatcaaag gttaatgtgc gcgcacgcgc gcgaggatta
     7861 aggacggata aactccatta cgattaagta ggtaaggagg atgaaggcat gaaacgttca
     7921 cccaaagttg caatctacgt aactctggtc gtatttctgg tcatttcgtt gttgacagcc
     7981 tctgccgccc aatcggctac acgtaaacag gccaaagggg tcaaactcac ctttttgatg
     8041 gattggaacg gcacagcttc ctcaaaggga atgcccgact caatcaataa tcctgtggcc
     8101 cggaagatca aagaaaaaac cggtataaca gtggacattg aatatgcgac caccaatgag
     8161 gccgaaagac tgaatctcat gttcgcttcg gggaacatgc ccgatattgt aaacgcacct
     8221 ttctggggcg ggagcaatgc ggaaacgatc gccatcaaga aagcggctac cgaaggtcta
     8281 ctgcttaatt tggatcccct catcgcaaaa tacggtccca acctgaaaga tgcattccag
     8341 aaaggacttg ccatcggcta taaggaaaat gacatcaacg atccggcctt cgggggcaaa
     8401 catttcgtcc taccccagca ggctccggcc accaaagacg atgtactcaa ctggggcggc
     8461 ggtttatatt gccgcaagga tatactcgat aaactgaaaa tcaatcccgc gagcattaac
     8521 agctcggaag ccttgtataa tctgttgaag gcgattaaaa aaggtgagtt taaggatatc
     8581 aacgggaaag cggtaattcc cgccggtgcg tggggtgatg gctgggacta tgtcgagttt
     8641 tggaggcctt tccgccagga ccgcagagcg gaattcgatt atatcgatgg taaattcgta
     8701 taccgggcca ataaccccct gcttgatcaa agggtactat ttatgagaaa actgattacg
     8761 gagggattgt tcgatcccga atgcctccgc caatcagatt ctcaggccaa ggaaaaaatg
     8821 gcaaccggcc gggtagccat ggtaggctgc cactattttc acatccgcga tttcatgaat
     8881 aacacgcttt ataagaccaa tccccaaatg cattatataa ctatcggtcc gctgccctgg
     8941 tccgacggga taaccaccgt ggttgaagta ccggatcgca acggcacacc ggtcattttc
     9001 ctccccaaga cctgcgccga tccggtggcg gcgatccggt tcctgaatta cctcaattcg
     9061 gatgaaggcc agttactggc ctactatggc attaaaggaa cccattatac catggtgaac
     9121 ggcaagccaa gaatggcaaa gaagtggctg gatatatgga agagcgatcc taaaaaactc
     9181 caggctctcg ggatcaggtc cacgttcatg gatatgattt gtttggataa gaggctgagt
     9241 gcctatggcg agctgctgcc cggagaagcg gacaatccgg atccgtggta tcaacaagcc
     9301 aagtcggtac ggcgtatcga gttcaagtcg ggacatacaa ttaacgatat taccaatcag
     9361 tatcccagac tggatgcggt gagatcttta gtcgactgga atctttaccg ggattccacc
     9421 gaaagggcat actttgcgaa atccgatgaa gaggcgttga aaattttgaa cgatttccgg
     9481 gaacaa
//
LOCUS       DASVMB010000153         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_313408,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000153 DASVMB010000000
VERSION     DASVMB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313408"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VF531_04720"
     CDS             complement(<1..949)
                     /locus_tag="VF531_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7713303.1"
                     /translation="MAGQNVAIYLDFENLAISAEEVYPSKDKPLVIEPVVDFATTKGN
                     VCIKKAYADWSNDSFSQYENVLMEQGFELIHLPATNQQGKNGSDVRLAIDVMENMELF
                     QTINTVIIGSGDTDFVPLIQRIRARGMQVISIGFDHSVGNLVKINSAEFKSLEELIGK
                     PDEESLSSDLAQEMDGSYGRELLIRYIRNRNNDEPVLLATLKQDLLRLDPSFSERKLG
                     FSSFKKFAQSFVGDLVNRVDPTEKDGLLLVYLNDVDLPQNQRTNMKDEAKVFLAKNVR
                     FQKNKAKRLAISDSVLDEYKENREMTMSQMIELTSARLKN"
     gene            complement(1003..1482)
                     /locus_tag="VF531_04725"
     CDS             complement(1003..1482)
                     /locus_tag="VF531_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713304.1"
                     /translation="MPDMLVRLYHLPTGLSDFPDLARDGIEIRRALPPDKSRIVRWVT
                     EHFNESWGSECDVTFSNKPVSCFVAVENKVKIVGFACYEATCKDYFGPTGVAADFRGR
                     GIGKALFLRCLYAMREMGYAYAVIGGASEAIEFYHKTADAFVIPDSVPGIYRDMIGS"
     gene            complement(1732..3681)
                     /locus_tag="VF531_04730"
     CDS             complement(1732..3681)
                     /locus_tag="VF531_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX7713305.1"
                     /translation="MSLNRQPSISLAEIKITAGFWSGRVELIAKEVIPYQWEALNDTI
                     PGAEPSHALENFRIAAGERTGEFHGMIFQDSDVAKWIEAASYSLATHPDADLGRKIDT
                     VIGWMEKAQQPDGYLNTYFTVAKPEGRWKDFRSAHELYCAGHLMEAAVAYYEATGKRK
                     LLDIMCRYADYIDSVIGPEPHKMRVYCGHPEIELALIKLYRATGVRRYLELSRYFIDE
                     RGKQPSFFTKEPGFSKSDPWFNLDYHQAHAPVRQQVTAEGHAVRAMYLYSAMADLALE
                     NSDETLVAGLKKLWENVTGRRMYITGGLGSQGYGERFTFDYDLPNDTAYAETCATIGL
                     IFWAQRMFRLDPDRKYTDVMERALYNGALSGISLDGMSYFYVNPLEVFPEAAARRHDL
                     EHVKPERRQWFGCACCPPNIARLIGSLGQYIYSREDERLYVHLYIGNEARFSMHGTPV
                     SLKLQTDYPWDGRIILQVHPEQPVSFALGLRIPGWCRKFSLAINGAAVARPVLNGGYL
                     FLEREWSAGDQVELVLDMPVELMQANPLVREDAGRVAIERGPLVYCLEETDNGANLPD
                     ISMSREAEFEVEYAPDLLGGITVIKGKAGRAVGDGWDNALYQPVNTHARLHDRFRPVT
                     VTAIPYAFWGNRIPGEMLVWIRLNG"
     gene            complement(3705..5213)
                     /locus_tag="VF531_04735"
     CDS             complement(3705..5213)
                     /locus_tag="VF531_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEX7713306.1"
                     /translation="MKYAQCIIEKEYHIGNTDPRLFGSFIEHLGRAIYTGIYEPAHPT
                     ADEQGFRKDVLDLVKQLQVSIIRYPGGNFVSGYNWRDGIGPVQDRPSRLELAWKSIET
                     NEIGIDEFADWCKKAGSQVMEAVNLGTGTPADAGYLVEYCNHPAGTYWSDLRRQNGHE
                     QPHHIKTWCLGNEMDGSWQICRLSADDYGKKALEAAKIMRWVDSGIELVACGSSNTDM
                     PTFPEWDRIVLEYLYDQVDFISLHRYYGHDDYDNLGDFLASFVDMDHFIHTITATADY
                     VQAKKRSKKVMYLSFDEWNVWAHRQESFKTWDIAKPRLEQVYNLADALVMGGLLCSLL
                     NHADRVKMACLAQLVNAIAPIYTQPGGAVIKQTIFYPFQQVSVYGRGSVLKPLITSPN
                     YDSKIYGQVPILQAAVVFDQSAGEVNLFILNCDQLEDVEVTLDFRSFGSARLLEHVTL
                     NGLDLTAINSFAEPEKVIPRSLSISGNHAERHTAVLPKLSWNMLRFSAGVDR"
     gene            complement(5188..>6518)
                     /locus_tag="VF531_04740"
     CDS             complement(5188..>6518)
                     /locus_tag="VF531_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7713307.1"
                     /translation="SGYHWSDGIGPEEDRPARLNLAWKSLESNRFGFDEFVDWAKKAG
                     AETMAAVNLGTGTPEDAGYLVEYCNHPSGTKWSDLRRKNGHDQPHNIKIWCLGNEMGG
                     YWQIGRLSAEDYGKKAMETAKMMRLIDPSIELLACGSSTTRLSSYPEWDRVVLEYLYD
                     QIDYISIHHYYGYDNELGDYLGSFVDMDRFIRTIIATTDYVKALKRTPKILGLSFDEW
                     NVWYHQPISFPTWEWDTAPKRLEQIYTLLDALVVGGLICTLLRHADRVKIACVAQLVN
                     VIAPILTGPGGRVIKQSIFHPLQQVSIYGRGQVLNPLMTCPRYASKMYGDVPILQLAA
                     VFNQDAGMVSLFILNCDQWEDTELSLDFRSFGPAELLEHILLNGPDLGAANSFSDPER
                     VKPRALPVSPMNTDQISVVLPKLSWNMLRFSLLTSDAGKGSPNEVRAMHY"
BASE COUNT         1605 a         1840 c         1522 g         1551 t
ORIGIN      
        1 tattctttag cctggcactg gttaactcga tcatctggct catggtcatc tcccggttct
       61 ctttgtactc atcgagcaca ctgtcggaga tggccagtcg tttcgcttta ttcttctgaa
      121 aacggacatt cttggccaaa aagactttgg cctcatcctt catattggta cgttggtttt
      181 gggggagatc cacgtcattt aaatagacca ggagcaaccc gtctttttcg gtgggatcga
      241 cccgatttac caggtcaccc acaaaagact gggcaaactt cttaaaagaa gagaaaccaa
      301 gttttctctc ggagaaggag gggtccagac gaagcaagtc ctgtttcaat gtggctagta
      361 acaccggctc gtcattattt cgattacgga tgtaacggat taaaagttcc cggccatagg
      421 atccgtccat ctcctgggcc agatcggagg agagcgactc ctcatcgggt tttccgatca
      481 attcctccag ggacttaaat tcggcgctat tgatcttcac cagattgccg acgctatgat
      541 cgaatccgat gctgatcacc tgcatccccc gggcccggat cctctggatc agcgggacga
      601 agtccgtgtc cccggaaccg atgattacag tattgatcgt ctgaaacagt tccatattct
      661 ccatcacgtc gatggccagc cggacatcgg atccgttttt accctgttga ttggtggccg
      721 ggagatgaat caactcaaaa ccctgttcca tcaggacatt ctcgtattgg gagaacgaat
      781 cgttggacca atcggcgtat gcctttttaa tacagacatt ccccttggtg gtggcaaagt
      841 ccaccaccgg ttcaataact agcggcttgt ctttggaggg ataaacctcc tcggcggaga
      901 ttgccaggtt ctcaaagtct agataaatcg cgacattttg accggccatg ggtaactcct
      961 ttacggatta tttatacttt acctttcccg gttccctgtc catcaagacc caatcatatc
     1021 ccggtaaatc cccgggaccg aatcgggaat tacgaaagcg tcagcggttt tatggtaaaa
     1081 ctcaatcgcc tccgaggctc ccccgatcac cgcataggcg taacccatct ctctcatggc
     1141 ataaaggcag cggagaaaca gggccttccc gatgccgcgg ccgcggaagt cggcagctac
     1201 gcctgtcggg ccgaagtaat ccttgcatgt cgcctcgtaa caagcgaaac ctacgatctt
     1261 taccttgttt tccactgcta cgaaacaaga aaccggttta ttactaaatg tgacatcgca
     1321 ttcgcttccc cacgactcat taaaatgttc ggtaacccaa cgaacaatcc tgctcttatc
     1381 agggggcagg gcccggcgga tctcaatacc atcccgcgcc agatcgggaa agtcggaaag
     1441 ccccgtcggg agatggtaaa gccttaccaa catatccggc atcctcgtgt catcctttct
     1501 cctaaacctt tggttggctt gggcccgaac actgcccgct ttgaaaggaa aaaccatatt
     1561 ccggtaatct tattctgtag attcacgacc gattcctgcc ccccgcccta taaaatatta
     1621 accccggaat catcggaaat tacacgtagt gtgcgcgcct tcctccgggg ttatttcgat
     1681 caacacctcg caacggttga atgcttgagc aaatttctac ttggctatat ctcaaccatt
     1741 caaccggatc cataccagca tttcgcctgg aatgcggttt ccccagaaag cataagggat
     1801 cgcagtgacc gttaccggcc ggaatcggtc gtgcaaacgc gcatgcgtat ttaccggttg
     1861 gtacaatgcg ttatcccatc catcaccaac cgcacgtccg gcttttcctt taatgactgt
     1921 gattcccccc aataaatccg gggcatactc cacctcaaac tcagcctccc gggacatgga
     1981 gatatccgga agattcgcac cgttatccgt ctcctccagg caataaacga gcgggccccg
     2041 ttcaatggct acccggcccg catcctcccg caccagggga ttggcttgca tcagctctac
     2101 cggcatgtcc agaaccagtt cgacttgatc cccggcggac cattcccgct caaggaagag
     2161 atatccaccg tttaataccg gccgggccac cgctgcaccg ttgatcgcta aagaaaactt
     2221 ccggcaccag ccgggaatcc gcaaccctaa ggcgaatgag actggctgct ccggatgaac
     2281 ttgcaggatt atcctgccat cccaggggta gtcggtttgc agtttcaggg atacgggcgt
     2341 tccgtgcatc gaaaaacggg cttcattacc gatatacaag tgaacatata accgctcatc
     2401 ctcccgggaa taaatatatt gccccaggga cccgatgagt ctggcgatat tcggcgggca
     2461 acaggcgcaa ccgaaccatt gccgacgttc cggtttaaca tgctccaagt catgacgtct
     2521 ggccgcggcc tccggaaaga cctccagcgg attcacataa aagtagctca ttccgtccag
     2581 ggaaattccg cttaaggccc cgttatacaa agctcgctcc atcacatcgg tgtattttcg
     2641 atccggatca agccgaaaca tgcgctgagc ccagaagatc aggccgatgg tggcgcaggt
     2701 ctcggcatag gcggtatcat tggggagatc ataatcgaag gtaaaccgtt caccataccc
     2761 ctgggacccc agaccgccag tgatatacat ccgcctcccg gttacattct cccagagctt
     2821 tttcagaccg gcaaccagcg tctcatcgct gttctccaaa gccaaatcgg ccatggcgga
     2881 atacaggtac attgcccgca ccgcatgccc ctccgcggtg acctgttggc gaacgggggc
     2941 atgggcttga tggtaatcaa ggttaaacca tgggtcactc ttgctaaagc ccggctcctt
     3001 cgtgaaaaac gaaggctgtt taccccgttc atcgatgaag taccggctca gctccaaata
     3061 acgtcgcaca ccggtggccc ggtacaattt gatcaaagcc agttcaatct cgggatgtcc
     3121 gcaatacact ctcattttat ggggctccgg gccgatcacc gaatcgatat aatccgcgta
     3181 gcggcacatg atatcgagta gcttgcgttt cccggtagcc tcataatacg ccaccgccgc
     3241 ttccatcaag tggccggcac aataaagctc atgagcggat cgaaaatctt tccaacggcc
     3301 ttcgggtttg gcaactgtaa agtaggtatt caggtatccg tccggttgtt gggccttttc
     3361 catccagcca attaccgtat cgatttttct tcccaaatcg gcgtccgggt gcgttgccag
     3421 actgtaacta gctgcctcga tccacttggc cacatcgctg tcttgaaaga tcatgccgtg
     3481 aaactctccc gttcgttccc cggctgctat ccggaaattt tccagcgcat gactgggttc
     3541 cgcgcccgga atcgtgtcat tcagggcttc ccattgatac gggatgactt ctttggcaat
     3601 gagttccacg cggccggacc aaaacccagc ggtgatcttg atctctgcca gtgaaattga
     3661 gggctgccta ttgagactca taaatcctca tctccattcg aaaattaccg gtcaactccg
     3721 gccgaaaaac gaagcatatt ccaggagagc tttggcagaa ccgccgtatg ccgctccgca
     3781 tgatttccgg atatcgacag acttctagga atgaccttct ccggttcagc aaaactgttt
     3841 atcgcagtga gatccaaccc attcagggtc acgtgctcaa gaagccttgc ggaaccaaag
     3901 gagcgaaaat ccaatgtaac ctctacatct tccagttggt cgcagtttag gatgaagagg
     3961 ttgacttccc cggctgattg atcaaaaacc accgccgctt gaaggatagg gacctgaccg
     4021 tatattttgg aatcgtaatt tggggaagtg atcaagggtt ttagaactga acctcttcca
     4081 taaactgaaa cttgctggaa cggatagaaa attgtttgct taataaccgc cccgccaggc
     4141 tgggtgtata tcggggcaat ggcattaacc agttgcgcca ggcacgccat cttcacccgg
     4201 tccgcgtggt taagtaaaga gcagagtaac ccgcccataa ccaaggcatc cgccaggtta
     4261 tatacctgct ccaaacgggg tttggcaatg tcccaggttt taaaagattc ctgccggtgc
     4321 gcccagacgt tccattcatc aaatgaaagg tacatcactt tcttgctccg tttttttgcc
     4381 tggacatagt ccgcagtcgc ggtgattgta tgaataaagt gatccatgtc gacaaaggag
     4441 gcgagaaaat cgccgaggtt atcgtaatca tcatgaccat aataacgatg gagcgagata
     4501 aaatcaacct gatcatagag atactctaag acgatccgat cccactccgg aaaagtcggc
     4561 atatccgtat tggagctgcc gcaagccacc aactcaatcc ccgaatcgac ccatcgcatg
     4621 atcttggctg cttccagagc ctttttaccg tagtcatccg cggataaacg acatatctgc
     4681 caggaaccgt ccatttcgtt tcccaggcac catgttttga tatgatgggg ctgttcatga
     4741 ccgttctggc ggcgcagatc gctccagtaa gtcccggcgg ggtggttaca gtactccacc
     4801 agatatccgg cgtcggcggg tgttcccgtc cccaggttaa ccgcctccat cacctgggaa
     4861 ccggcttttt tacaccaatc ggcaaactca tcgatcccga tctcattggt ttcgatcgac
     4921 ttccaggcca gctcgagccg gctgggacga tcctgaaccg ggccaatccc atcccgccag
     4981 ttatacccgg agacaaaatt gccgccggga tagcgaatga ttgacacctg caactgtttc
     5041 accaaatcaa ggacatcctt gcgaaatcct tgttcatcgg cagtcggatg agccggctcg
     5101 taaatacccg tataaattgc ccggccgagg tgctcgatga acgaaccgaa aagccggggg
     5161 tcggtattgc caatatgata ttccttttca ataatgcatt gcgcgtactt cattcggaga
     5221 cccctttcca gcatctgatg taagcaaact gaaccttagc atattccatg acaactttgg
     5281 cagtacaacc gagatctgat cggtattcat tggagaaacc ggcaaagctc tcggcttgac
     5341 ccgctccgga tcggagaaac tgtttgccgc tccgagatcc ggaccgttca ggagaatatg
     5401 ttccaaaagc tcggctgggc cgaaagacct gaaatctaaa gagagctctg tatcctccca
     5461 ttggtcacag tttaagatga acaagctgac catcccggcg tcttgattga agaccgccgc
     5521 caattgcagg atcggaacat ccccatacat tttcgacgca tatcttgggc aagtcattaa
     5581 tggatttaaa acttgccctc tcccgtagat cgagacttgc tgcaacggat ggaagatact
     5641 ctgtttgatc acccggccac ccggcccggt aagaatcggg gcgatcacat tgaccaattg
     5701 cgccacacaa gcgatcttca cccgatcagc atgacgcaat aacgtacaga ttaagccacc
     5761 cactaccaag gcatccaata atgtatagat ctgttccaat cgcttgggcg cagtatccca
     5821 ctcccaggtt ggaaaactaa tcggctgatg ataccaaaca ttccattcgt caaacgacaa
     5881 ccccaggatt ttaggggttc gtttgagtgc cttcacataa tccgtggttg cgataatcgt
     5941 gcgaataaag cgatccatat ccacaaacga acccaaatag tcccccaatt cattatcgta
     6001 accgtaataa tgatggatag aaatgtaatc gatctgatcg tacagatatt cgagtacaac
     6061 gcgatcccat tccgggtaag aagacagccg cgtggttgaa gaaccgcaag caagcagctc
     6121 aatggacgga tcaatcaagc gcatcatctt ggctgtttcc atggctttct tgccgtaatc
     6181 ttccgccgac agccggccaa tctgccagta gccgcccatt tcgtttccca aacaccagat
     6241 tttgatatta tggggctgat catggccgtt tttgcgtctg agatcgctcc actttgttcc
     6301 ggaaggatga ttgcagtatt ccaccaggta accggcatcc tccggagtcc cggtgccaag
     6361 attgactgcg gccatcgtct ccgcgccggc tttcttagcc caatcgacaa attcatcaaa
     6421 gccgaaccgg ttggactcca acgatttcca ggctaaattt agccgcgcgg ggcggtcctc
     6481 ctccggaccg atcccatcgc tccaatggta accggaga
//
LOCUS       DASVMB010000154         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_387554,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000154 DASVMB010000000
VERSION     DASVMB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387554"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF531_04745"
     CDS             <1..265
                     /locus_tag="VF531_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase"
                     /protein_id="HEX7713308.1"
                     /translation="GGNGWAAKQGIPVYPIFGQTVNYLGYNGVFELARRLVRLLKNPV
                     FNKNLAANTRLPYFEDWYRQDPFTYIDSKTALADSSLEKSEAG"
     gene            262..1137
                     /locus_tag="VF531_04750"
     CDS             262..1137
                     /locus_tag="VF531_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7713309.1"
                     /translation="MIAKETFDHLVKQHPCFNREAHFKYGRIHLPVSPSCNIQCRFCK
                     RGFNKWEDRPGVSRLLLNPGEAVAKVASALAICPQIRVVGIAGPGDTLATDHALEAFA
                     AVHQRYPALINCLSTNGLLLEEKAERITAAGVKTVTVTVNAVDAGILQMICSHVRYHE
                     QLLCGEHAARLLISAQIAGIRKIVRLGAMVKVNTVLIPGVNDHHIGHIASVASKLGVS
                     MFNIIPLIPQHEFARLHPPDCDELAKARQAAEEYLPVFRHCRHCRADACGIPGSGKDL
                     GGNLYEQDGETFSHG"
     gene            1206..2087
                     /gene="nifH"
                     /locus_tag="VF531_04755"
     CDS             1206..2087
                     /gene="nifH"
                     /locus_tag="VF531_04755"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HEX7713310.1"
                     /translation="MSEKKRIKQIAIYGKGGIGKSTTTSNISAALAKAGHKVLQIGCD
                     PKSDSTNTLRGGKYIPTVLDTLREKNTIKAEEVVFQGFSGVYCVEAGGPAPGVGCAGR
                     GIITAVQLLKELKVFEELNVDIVLYDVLGDVVCGGFAVPIREGIAEHVFTVSSADFKA
                     IYAANNLFKGIQKYSDSGGALLGGVIANSINTPYAKEIIDDFVAQTETQVIGYVPRSV
                     TVTQSELQGKTTIEAAPDSEQARVYQRLAQRIAEHARSKTPLPLDVKELREWAARWAD
                     NLLALETGEVRDAAASI"
     gene            complement(2174..2437)
                     /locus_tag="VF531_04760"
     CDS             complement(2174..2437)
                     /locus_tag="VF531_04760"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX7713311.1"
                     /translation="MMHWMWYNGWQSGWGGLIMGVMMILFWAAVIGGIYLVIRSLIRS
                     GSLYRPERTPMDILQERYAKGEISDADYDRIKQKLNEDRKVIK"
BASE COUNT          743 a          803 c          945 g          769 t
ORIGIN      
        1 cggtgggaac ggctgggcgg ccaaacaagg tatcccggtt taccccatct tcggccagac
       61 cgttaattat ttgggttata acggcgtctt cgagctggca cggcggctgg tccggctgtt
      121 gaaaaatccg gttttcaaca aaaatctggc agccaatacc cgtttgccgt attttgaaga
      181 ctggtaccgg caagacccct ttacttatat cgacagtaag accgccttgg cggactcgtc
      241 actggaaaag agtgaggcgg gatgattgcc aaggaaacgt ttgatcacct ggtcaaacaa
      301 cacccctgtt ttaatcgcga ggcccatttc aagtacggcc ggattcacct gccggtgagc
      361 ccgagttgca atatccagtg ccgtttttgc aaacgtggtt ttaacaaatg ggaggaccgg
      421 cccggagtca gccggttatt gctgaatccc ggggaagcgg ttgccaaagt ggcctcggcc
      481 cttgcgattt gtccccagat ccgggtagtg ggaatcgccg gtcccggaga tacattggcc
      541 acagatcatg ccctggaggc gtttgcggcg gtacaccaaa gatatccggc gttgattaac
      601 tgcctgagca ccaatggact tctcctcgag gaaaaggcgg agcggattac ggcggccggg
      661 gtcaaaaccg tgacggttac tgtgaatgca gtggatgccg ggattttgca gatgatctgc
      721 tctcatgtcc gctatcatga acagctttta tgcggtgaac acgcggcccg cctcctgatc
      781 tcggcccaga tcgccggaat ccgcaagatc gtccggttgg gcgcgatggt caaagtcaat
      841 acggtcttga tcccgggagt caatgatcat catatcggcc atatcgccag tgtagcctcg
      901 aaactgggcg tttccatgtt caacatcatc ccgctcattc cgcaacacga gtttgcgagg
      961 ttacatccgc cggactgcga tgaactcgcg aaagcgcggc aggcggccga agaatacctc
     1021 ccggtttttc gccattgccg gcattgccga gccgacgctt gcggcatacc cggttcgggg
     1081 aaggatttgg gagggaacct ttatgagcag gacggggaga ctttttccca cgggtaatct
     1141 caaccgtctt atttcttacc atcgggacga agttttcaat tcatatcatc taaggagtga
     1201 agaccttgtc ggagaagaaa aggatcaaac aaatcgcgat ttacggcaaa ggggggatcg
     1261 gcaagtccac gacgacctcc aatatcagcg ccgcgctggc caaagccggc cataaggtgc
     1321 tgcagatcgg gtgcgatccc aaaagcgatt ccaccaatac gttgcgcggc ggaaaataca
     1381 tccccactgt gctcgacacc ttgcgggaga aaaatacgat taaagcggag gaggttgtgt
     1441 ttcagggttt tagcggagtc tattgcgtcg aggccggggg accggctccg ggagtaggct
     1501 gcgccgggag gggaatcatt acggcggtgc agctcttgaa ggaactgaag gtctttgagg
     1561 agttgaatgt ggacattgtg ctttacgatg ttttggggga tgtcgtctgc ggcggtttcg
     1621 cggtacccat ccgggaaggc atcgccgagc atgtctttac cgtgtcgtct gcggatttta
     1681 aggccatcta cgcggcgaat aatctgttca agggtatcca gaagtattcc gacagcggcg
     1741 gggctttgct gggcggggta attgccaatt ccatcaatac tccgtacgcc aaggagatca
     1801 tcgacgattt tgtggctcaa accgaaacac aggtgattgg ctatgtcccg cgatcggtaa
     1861 ctgtgaccca gagcgagctg caaggcaaga ccaccatcga ggcggcgccc gattcggaac
     1921 aggcccgggt ttaccaacgc ctggctcaga ggatcgctga gcatgcgcga tccaaaacgc
     1981 cgttgccgtt agatgtcaag gagctgcggg aatgggcggc ccggtgggcc gataatctgt
     2041 tggcgctgga gaccggggaa gtccgggacg cggcggcaag catttaggtg aagcgcgtat
     2101 ccaaataagg gggctgtccc aaagagaaca aaggttcttt taggacagcc ccacctcttt
     2161 acgcgggcct attttactta atcacctttc tatcttcgtt cagtttttgt ttgattcggt
     2221 cgtagtccgc gtcgctaatc tcgcctttgg cgtagcgctc ctggagaata tccatagggg
     2281 tgcgttccgg acggtagaga gaaccgctgc ggatcagaga acgtatgaca agataaatgc
     2341 cgccgatcac cgcggcccag aagaggatca tcattacgcc catgattaat ccgccccagc
     2401 ctgattgcca tccgttatac cacatccaat gcatcatctt cgttagctcc tttgtcggtt
     2461 tgtatacttt gaatatgccc cgaatgttta cgatattatt cggtcccctc tcccacgaaa
     2521 acaccggggt catgatggga gcccgggttc cttctccggc catccgctgc cggagaggtg
     2581 aaccatagaa ggcagtaggt gaagggtttc ttttgaatga gattagtgta acaaaaagat
     2641 gtggagattt gatggagact gtgctaagtt catgtgaatg taccagactg tgatggtaca
     2701 tgccccatga ttgcaggaaa aatgggattg atctagtaat catatcatat cggtatggaa
     2761 gtgaaacgct tgagtgagta aaggcgcttt cggtgttctg tggggaatat gacggaatag
     2821 tgaaggcgtt ttttggatat cgggtaatgt tacaacaagt tttacgggta tggtgacgga
     2881 aaatgagagt gcgggcgtag aatttatttg tggcttggtc ataatcgccg aaggggaacg
     2941 gcgcgaacaa tgttcgcgaa cccacttccc cgcattagag ccaagggctg tggccggacc
     3001 ccctatgaag tttgaacttg gatagtgagg aattgtatta tacactttgg cctatcctaa
     3061 actgaatgtc gacattgtcc aggggtcgtt cggtcgaccc ctaggaccta cccgcccgcc
     3121 ggggaggagg ctccccggac ccctgctata tctcctccca tccgaggtcg gagaaggagt
     3181 ttttgattta actggccacc gtctccatta cggtggcctt cttgggtggt ttagtaggcc
     3241 gacggctgtg attcttcctg
//
LOCUS       DASVMB010000155         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_683022,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000155 DASVMB010000000
VERSION     DASVMB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683022"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF531_04765"
     CDS             complement(<1..214)
                     /locus_tag="VF531_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713312.1"
                     /translation="MIANSSVARRFPWIGKLDRELIILLISGLVTIAVIPFGFAAQSV
                     TVPEHSSFFAFESPEEVKIRLIAQTIV"
     gene            450..1370
                     /locus_tag="VF531_04770"
     CDS             450..1370
                     /locus_tag="VF531_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7713313.1"
                     /translation="MEIQANAVFEGGGVKGIALVGAVCYLEQMGYGWRKLAGTSAGSI
                     VAALLAAGYSGRELADLIQYQNFIEFQDAGCWERLPMIGPALSLLFKLGIYRGRYFEA
                     WLGKLLKARGIVTFKDIKRTGRTLKVIASDLSRRKMLVLPDGLREYGVEPDGFSVARA
                     VRISIGIPLYFEPIHLRYRKDGVLRLSYLVDGGLFSNFPLWLFDRDGEEVPLFGFRLF
                     QPGREKETAIRGPIGFLKAIVASLLESQELQHIHEKDWVRTIAIPTQSVGVTDFNITP
                     EQKRMLFQSGWQAARAFMTGRSIQWKKVTR"
     gene            complement(1404..2918)
                     /gene="gcvPB"
                     /locus_tag="VF531_04775"
     CDS             complement(1404..2918)
                     /gene="gcvPB"
                     /locus_tag="VF531_04775"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7713314.1"
                     /translation="MNNMNKAMNPMETGNPSLQLIFEKSVPGKRAWQLPKSTVPQYPI
                     ARMVDSRQLRATSPNLPELAEIDLVRHYTGLSRRSHGVDTGFYPLGSCTMKYNPKINE
                     AVAEWKEFTRIHPLAPAKLSQGTLTIFKELEKGLAEITGMDRFTLQPAAGAHGEYTAL
                     LIVQAYHKSRGQTGRTKILVPTSAHGTNPASAAMAGFEAIPVNCNADGLVDLDHLRSL
                     IGPNIAALMLTNPNTLGLFERDIAAIAELVHSCGGLLYYDGANLNAIMGLVRPGDMGF
                     DLIHLNLHKTFATPHGGGGPGAGPVGVKSFLAGFLPVPLVEQGREGYYWDYDRPQSIG
                     KIQGFYGNFLVLLKALMYIKALGGPGLTQVSRDAILNANYLRAQLHEAYQIPYDRPCM
                     HEFVLSATNQKEYQVRALDIAKRLLDFGVHPPTIYFPLIVEEAMMIEPTETEDLQTLN
                     RFTQIMLQIAREAAETPEIVREAPHSTSVNRLDEARAARTPILRFQPGQPDTGR"
     gene            complement(2911..>3215)
                     /locus_tag="VF531_04780"
     CDS             complement(2911..>3215)
                     /locus_tag="VF531_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HEX7713315.1"
                     /translation="LREIAARCFNNAHYLYERLINSGFTPLYPDKPFFMEFALRTPEG
                     ADYYINRLINTGILAGYDLSRSHPERDPAILICATEIFSREQLDEFVTAMEGCRHE"
BASE COUNT          737 a          738 c          928 g          812 t
ORIGIN      
        1 agacgatggt ttgagcaatc aaacggatct ttacttcctc cggactttca aaggcgaaga
       61 aggaggaatg ttccggtacg gtaacggatt gggcggcaaa cccgaaagga atcaccgcaa
      121 ttgtcaccaa tccgctgatt aataagataa ttagttcccg gtcgagcttg ccgatccagg
      181 gaaacctcct tgccacgctt gaatttgcaa tcatgatccg ccctcctcga tggatgagat
      241 tcaaatggta aagaaccgca attcgcatta taacgtttac ggttgcatta ttggccggaa
      301 atttttatat tataccccga tctatcatct agtgtgacaa cctcctgaat ctcgacccgc
      361 gtacaccatc acttgtggtc cattggaacc gtataatgaa tggaacggcc tggtgagatc
      421 ctggattttt acgggtaagg aggaccactt tggaaataca ggcgaatgcc gtttttgagg
      481 gcggcggagt gaaaggaatc gcgttagtcg gtgctgtttg ttacttggaa caaatggggt
      541 atggttggcg gaaattggcc ggaacctccg ccggatcgat cgtagctgct cttttggctg
      601 cgggatattc cggaagagaa ttggcggatt tgatccaata ccaaaatttt attgaatttc
      661 aagacgccgg gtgttgggag cggttgccga tgatcggtcc ggccttgagt ctcctattta
      721 agcttgggat atatcgtggg cggtattttg aggcctggct tgggaagttg ttaaaagcca
      781 gaggaatagt gacttttaaa gacattaaaa ggaccggacg gaccttaaag gtaattgcca
      841 gtgatcttag tcgccggaag atgctggtgc ttccggacgg attacgggag tatggtgtgg
      901 aaccggatgg attttcagtg gcgcgggcgg tccggatcag tatcggaata cctctgtatt
      961 ttgaaccgat tcatttacga taccggaaag atggtgtgtt acggttaagt tatttggtgg
     1021 atggcgggtt attcagcaat tttccgcttt ggctctttga ccgggacggt gaggaggtac
     1081 ctctgttcgg attccggctt tttcagccgg ggcgggagaa ggagactgcc atccgaggcc
     1141 ccatcggttt tctcaaggcg atcgtcgcaa gtctcctgga gagtcaggag ctgcaacata
     1201 ttcatgaaaa agactgggta aggacgattg ccatccctac ccagtcggtt ggagtgacgg
     1261 attttaacat tactccggaa cagaaacgaa tgttgttcca atccggatgg caggcggccc
     1321 gggcgttcat gaccggccgg tccatacagt ggaaaaaggt cactcgttaa atggttgccc
     1381 aggctgtttt tatccaatcc ctttcatctt ccggtgtccg gttggccggg ttgaaagcgc
     1441 aaaatcggag ttcttgcagc ccgggcttcg tccaaacggt ttacgctggt ggaatgaggg
     1501 gcttcccgga cgatttcggg tgtctccgcc gcttctctgg ctatctgcag cattatctgg
     1561 gtgaagcggt tcaaggtttg taggtcctcg gtttcagtcg gttcgatcat catcgcttcc
     1621 tcgacgatga gcgggaaata tatggtggga ggatggaccc cgaaatcaag caggcgtttg
     1681 gcaatatcca aggcccggac ctgatattct ttttgattgg tcgcggataa aacgaactcg
     1741 tgcatgcagg gccggtcata tggaatctga taagcctcat gaagttgggc gcgaaggtaa
     1801 ttcgcgttta ggatcgcgtc ccggctaacc tgagtcaaac ccgggccacc caaggccttg
     1861 atatacatca gcgctttaag gagaaccaga aagttcccgt agaatccctg gattttgccg
     1921 atactttgcg gtcggtcgta atcccagtaa tatccttccc ggccctgctc aacgagggga
     1981 acgggaagaa aacccgccag gaaggatttg actccaaccg gaccggcgcc gggaccgccc
     2041 cctccatgag gggtggcgaa ggttttgtga aggttcagat gaatcagatc gaaccccata
     2101 tctccggggc gaaccagtcc catgatggcg ttaagattgg caccgtcgta atagagtaga
     2161 ccgccgcagg aatggacgag ctcggcaatg gcggcgatat ctcgctcgaa taaaccaagg
     2221 gtgttggggt ttgtgagcat caacgcggct atatttggtc ctatcaggga gcggagatga
     2281 tcgagatcca ccaaaccatc ggcattgcag ttgaccggta tcgcttcgaa accagccatt
     2341 gctgcactgg cgggattggt accatgagcc gaggtgggaa cgaggatctt ggtgcgtccg
     2401 gtttgcccgc ggcttttgtg gtaagcctgg acgatcaaga gagcagtata ttcgccatga
     2461 gcacctgctg ccggttgcag tgtaaatcga tccattccgg taatctccgc caggcctttt
     2521 tccaactctt tgaatatcgt cagagttcct tgactcagtt tggctggagc taaggggtgg
     2581 atccgtgtga actccttcca ttcggctacc gcttcgttga ttttgggatt atacttcata
     2641 gtgcaggaac cgagtggata aaagcctgtg tccactccgt ggctgcgccg ggataagccg
     2701 gtataatgac gaaccaggtc aatctcggcc agttccggaa ggttgggcga ggttgcgcgt
     2761 aattgtcggg aatcgaccat tcgggcgatc gggtattgcg gcaccgtgga tttgggaagt
     2821 tgccaggccc gtttcccggg tactgatttt tcgaagatca attgcaggct gggattaccg
     2881 gtttccatcg gattcattgc cttattcatg ttattcatgg cgacaaccct ccattgcggt
     2941 gacaaattcg tcgagctgtt cacgggagaa gatttctgta gcacagatga gtatggccgg
     3001 atcccgctcc ggatgactcc ggctcagatc gtagcctgcg agaattccgg tgttaatcaa
     3061 gcggttgata taataatccg cgccttccgg ggtccttagg gcaaattcca taaaaaacgg
     3121 tttgtcggga tagagcgggg tgaaaccgct attgatgagc cgctcataaa gataatgggc
     3181 gttattgaaa caacgcgcgg cgatttcccg gaggc
//
LOCUS       DASVMB010000156         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_20055,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000156 DASVMB010000000
VERSION     DASVMB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20055"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1107)
                     /locus_tag="VF531_04785"
     CDS             complement(268..1107)
                     /locus_tag="VF531_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX7713316.1"
                     /translation="MGEAETIANTCMDTNPMVRTRQSLAGDLYRLGLRPGMTVLVHSS
                     LSSLGWVCGGPVAIVQALCDAITSAGTLVMPAHSGDYSDPCYWQNPPVPKEWWQTIRE
                     TMPAYEPEITPTRGMGAIPETFRTFPGVLRSSHPALSFTAWGKDAAVITRGHTLDNAL
                     GEGSPLARIYERDGWVLLLGVGYENNTSFHLAEYRIPVRAPFDAGAPVLEAGQRVWKR
                     YKDIEFHMEFFAELGREFEARESVSHPVIQGKVGSAAARLFPQRAAVDFAAEWLKDKY
                     ESS"
     gene            complement(1237..2112)
                     /locus_tag="VF531_04790"
     CDS             complement(1237..2112)
                     /locus_tag="VF531_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7713317.1"
                     /translation="MKRFGKAKMFGLFTLFFFIGWFSYFENNHLTVTKITIPLPDTVL
                     GFEGLRIVQLSDLHGKLFGRNQELLLAKIAGLHPDMIVFTGDLVDAGHYRETPGLMLL
                     GTLARRCPVFMVGGNHESGSGRFHDLAKRLKFHGVRVLRDERLILARGDERLALIGMD
                     DLPDIHGRWYSSDSWIRLRLGKLISGLEAGEGKTRTYKILLVHRPEKFAIYGESGVNL
                     VLTGHAHGGQIRLPWLGGLYAPGQGYFPKLTSGLYGLPNSQTKMIVNRGLGNSGFPQR
                     LGNCPEIGLIILKCF"
BASE COUNT          661 a          729 c          587 g          620 t
ORIGIN      
        1 aaggtcgatc agtttcaggc gaacccggtt tccgaagggc ccccggtgct ccggtgatag
       61 tagcgcattc accgcagccc gttctcccgg atttagggga ttgccgtcga aagtccggca
      121 ggaattacgc tccatgatga tgtcgtggct ggatttcatc aagatccctc ctggtttcaa
      181 atcttcaaaa tgtcaaaccg ttccgctatc tcactcgcct tgacgattcg tttcgattgc
      241 aactaaatca ggatggctgt aagtcaatca acttgattca tatttatctt tcaaccactc
      301 cgccgcgaaa tccaccgcgg ctcgttgcgg aaagagccgt gcagcggccg atcctacctt
      361 accctggatc actggatgac tcacgctctc gcgggcctca aactctctac ccaattcggc
      421 gaagaattcc atatgaaact cgatatcctt gtaacgcttc caaactcttt gtccggcttc
      481 tagtaccggc gcgcctgcat caaatggtgc ccgaaccgga atgcggtatt cggcaaggtg
      541 gaacgaagta ttattctcat atcccactcc caataacaac acccaaccgt cgcgttcata
      601 gatccgggcc aggggggaac cttctcccaa cgcgttatcc aaggtatgac cccgggtaat
      661 cacagctgcg tccttacccc atgccgtaaa ggataacgca ggatgggaac tgcgcagcac
      721 tccagggaag gtgcgaaagg tttccggtat agcccccatc ccgcgagttg gagtgatttc
      781 cggttcatag gcgggcatgg tttcccggat cgtttgccac cactccttag gcaccggcgg
      841 attttgccaa taacacggat cggagtaatc gcccgaatgg gccggcatta ctaaggtgcc
      901 cgctgaggta atcgcatcgc atagggcttg gacaatagcc accggaccac cgcagaccca
      961 gcccaaggaa cttaaggagg aatggaccaa cacggtcatg cccggtctta agcccagacg
     1021 gtataaatcc ccggccaggc tttgcctggt acgaaccatg gggttggtat ccatacaggt
     1081 gttggcgatt gtctccgctt cgcccatcag gtgaccctcc ctgcaaactt aaagcctttt
     1141 tttgttgatg gctaaaaccg ataatgactc tcccctcgtg agcgcgaacc cataaatatc
     1201 gatgctttta gctacactca ttcacagggt gagttgttaa aaacacttca ggattatcaa
     1261 gccaatctcc ggacagttcc cgagccgttg cgggaaacca ctgtttccca atccccggtt
     1321 gacgatcatc ttggtttggc tgttaggtag gccgtataac cccgaagtca acttaggaaa
     1381 gtatccctgt cccggagcgt acaatccccc aagccacggc agccgaatct ggccgccgtg
     1441 ggcatgaccg gtaagcacaa gattcacgcc gctttcgccg tagatagcaa acttctcggg
     1501 acggtgaacc aaaagaatct tatatgtacg tgttttccct tcaccggctt ccaacccgga
     1561 aataagtttc ccgagccgca gccgaatcca cgaatccgaa ctataccaac ggccatggat
     1621 atccgggagg tcatccatgc ctatcaaagc cagccgttca tcaccgcgtg ccaaaatcag
     1681 ccgttcatcc cggagtaccc gcaccccgtg gaatttgagg cgctttgcca gatcatgaaa
     1741 tcttcccgag ccggactcgt ggttaccgcc aaccataaac accggacatc tccgggcgag
     1801 ggtccccagt agcatcaaac ccggggtctc acgataatga ccggcgtcga ccagatctcc
     1861 cgtaaaaaca atcatatcgg ggtgtaatcc agctatcttg gccaacagga gttcctggtt
     1921 cctgccaaaa agtttgccgt ggagatctga aagctgcaca atccggagac cttcaaatcc
     1981 cagaactgta tcgggtaatg ggatcgttat tttggttaca gtcagatggt tattttcgaa
     2041 atacgaaaac caaccgatga aaaaaaacag cgtaaagagt ccaaacattt tagctttccc
     2101 gaatcgtttc atcgattcct gtttggccgc cttccgtgcc ggatattaac ctaatcattt
     2161 tataacaatt tgaggttgat ttccagtaaa tcctcttgaa ttaaaaatac gacaaaaccc
     2221 cgtttccctg aagcgttggt tcaccctgta tcaattaaga tataaccatt aaatgactta
     2281 gatcatcacg atttgatccc gggttaatcg cgattaaata ccggtttaat cttcaggatt
     2341 taaacttaaa caaacaaggt tttgaatggc aaaatatggc ggaaccttcc atcccctgca
     2401 ggaaaatcgt ccttgccggc gacacatccg ccactattgt tcaaacaaag ccaaaaacaa
     2461 tgaatggagg atgatttgat gctgtttcat tcgacccgta aacagagcac actttggatt
     2521 cccatggctt ttatcctggc cttactggtt tcccttggat ccatcaccgg atacgccgct
     2581 gccgctcccg tcaaaaa
//
LOCUS       DASVMB010000157         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_535980,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000157 DASVMB010000000
VERSION     DASVMB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535980"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VF531_04795"
     CDS             complement(<1..352)
                     /locus_tag="VF531_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX7713318.1"
                     /translation="MLKFGDEKGQLKCSFCGKAQEQVKKLIAGPGVYICDECIELCNE
                     IIDEELNEEVNSDLSSNPKPKEIKSILDQYVIGQEDAKKFLAVAVYNHYKRINSEERF
                     EDVELQKSNILLLGP"
     gene            complement(414..1013)
                     /gene="clpP"
                     /locus_tag="VF531_04800"
     CDS             complement(414..1013)
                     /gene="clpP"
                     /locus_tag="VF531_04800"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX7713319.1"
                     /translation="MNLVPMVVEQTNRGERAYDIFSRLLKERIIFIGGGIDDNMANLV
                     IAQMLFLASEDPDKDISIYINSPGGEVYSGLAIYDTIQYIRPQVMTICIGIAASMAAI
                     LLAAGTKGKRFALPNSRVMIHQPLGGAQGQAADIEIQAQEILRIREIGNEILAKHTGQ
                     SLDKIQRDVDRNFWMSATDAKEYGLIDDIFDSKKKVENK"
     gene            complement(1033..2340)
                     /gene="tig"
                     /locus_tag="VF531_04805"
     CDS             complement(1033..2340)
                     /gene="tig"
                     /locus_tag="VF531_04805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7713320.1"
                     /translation="MQFNLEKLDKNFVQLTATASSDEVDTALAEAYKRVVNKVNLPGF
                     RKGHIPRSVLEAHFGKEVLHEDALDILVTKGYFEAIEEHQLEPIDQPNLEIDKPIAEN
                     QPFIFKAKIQVLPEVKLGEYKGLKVEKPEVHVDDEQIAAQLKALQERHAELVLSDKQV
                     LENGDFAIIDFEGYVDGKAFPGGAAQSYTLEIGSKSFIPGFEEQLIGMKVGTAKEINV
                     SFPADYHSKELAGQEATFKVTLKEIKVKEIPGIDDEFAKSIGNFENVAQLKADLLVKL
                     THAAEHESESKFSQEVIDQAVENAKVEIPEVMVERELEDLTHRFEQNLAYQGLTMDRY
                     LEYAQKTREQVKEDFLPEAQKRVKTDLVLNSVAKTEQITVSEAELDEKLHELAHMYQV
                     KDPAAFRRDIEKKGRLGDIKQALLLEKTADFLKTQAIPKSVKK"
BASE COUNT          746 a          695 c          523 g          832 t
ORIGIN      
        1 tggggcctaa aagtaaaata ttgctctttt gaagctcaac atcttcaaag cgttcctcgg
       61 aattaattcg tttataatga ttatataccg cgaccgctaa aaactttttt gcatcttcct
      121 ggccgatcac gtattgatcg aggatactct tgatctcctt cggcttggga ttactgctta
      181 agtcggagtt gacttcctcg tttaattcct catcgataat ctcattacaa agttcaatgc
      241 attcatcaca gatataaaca ccagggccgg cgattaattt cttcacctgt tcctgggctt
      301 ttccacaaaa agaacatttt aactgacctt tttcatctcc gaattttaac attcattcac
      361 ctcatctctc tcggaatcgt accgtcatcc cccactcttc aaattaaatg gatctattta
      421 ttctcgacct ttttcttact atcaaagata tcgtcgatta atccatattc tttagcatcc
      481 gtggcggaca tccagaagtt acggtccaca tctcgctgaa tcttatctaa agattgaccg
      541 gtatgtttag cgagaatctc attaccgatc tcccggatcc gcaaaatctc ttgggcctgg
      601 atctcaatat ccgctgcctg gccttgagcg ccacccaaag gttgatgaat catcacccgc
      661 gagttcggta aggcaaaccg cttacctttg gtcccggctg ccaacaagat cgcagccata
      721 ctggccgcga ttccgataca gatcgtcatg acttgcgggc gaatgtactg gatcgtgtca
      781 taaatggcca aacccgaata tacctctccg ccaggactgt tgatatagat ggaaatatct
      841 ttgtccgggt cttcgctcgc caaaaaaagc atctgcgcaa taaccaggtt ggccatgttg
      901 tcatcgattc cgccaccgat aaagataatt cgctctttga gcaaacgtga aaagatatcg
      961 tacgcccgtt caccccggtt ggtctgctcg accaccatcg gtactaaatt catctccatc
     1021 aacttccatc cctcatttct taacggattt gggtatcgct tgggttttta aaaaatcagc
     1081 cgttttttcc aaaagaagag cttgtttaat atcacccaga cggccttttt tctcaatgtc
     1141 ccggcgaaat gcggcgggat ccttcacttg gtacatatgg gccaattcgt gtaatttttc
     1201 atccaactcg gcttcgctaa cagtgatttg ttcggttttg gcaacactgt ttaaaaccaa
     1261 gtcagtctta actctttttt gggcttcggg tagaaaatct tccttaacct gttcccgggt
     1321 cttttgggca tattcaaggt atcgatccat cgttaaacct tgataggcta agttttgctc
     1381 aaaacggtgc gttaagtcct caagctcccg ttctaccatg acttcaggga tttccacctt
     1441 ggcattttct accgcttggt cgataacttc ctgggaaaat ttggattccg attcgtgctc
     1501 ggctgcatgg gttaatttta ccaataagtc cgcttttaat tgggcaacgt tttcaaaatt
     1561 tccgatactt ttggcaaact cgtcgtcgat cccggggatc tccttaactt tgatctcctt
     1621 taacgtcacc ttgaaggtgg cctcttgacc ggccagttcc ttgctgtgat agtcggcggg
     1681 aaaacttaca ttgatctcct ttgcggtgcc cactttcatt ccgattaatt gttcctcaaa
     1741 tcccggaata aagctctttg acccgatctc cagagtataa ctttgggccg ctccaccggg
     1801 aaatgctttg ccatccacat aaccctcgaa atcaataatg gcaaaatcac cattttcaag
     1861 aacttgttta tcacttaaaa ccaactcggc atgccgttcc tgaagggctt tcaattgagc
     1921 cgcaatctgt tcatcatcca catgtacttc aggtttttcc actttcaacc ctttatactc
     1981 accaagcttg acttcaggca gaacctggat cttggcctta aaaataaatg gctgattctc
     2041 agcgattggt ttatcaatct ccaaattcgg ctggtcaatc ggttccagct ggtgttcctc
     2101 tattgcttca aaatatcctt tggtgaccaa aatatccagc gcatcttcat gtaaaacttc
     2161 ttttccaaag tgggcctcaa gtaccgaacg gggaatatgc cctttacgaa aaccagggag
     2221 attcactttg ttcacaaccc gtttataggc ttccgccaaa gccgtatcga cctcatcgga
     2281 acttgcggtg gcggtcagtt gaacaaaatt cttgtctagc ttttccaggt taaattgcat
     2341 ttatgctcct cccgaatcta tactatatat atacgtagaa tatacgtaac gattaacaaa
     2401 aacaaagtct tttcaccgcg gttaaaaaag ccaaaaactc cctataacag gatttcgacg
     2461 gtaagaaaca aattccttgt taccagtcgt aaaaccccca gggaatagaa tttgcatggg
     2521 ctgattaatt taaaaaaaac cgccaaatgc accagcttcc ggcatattaa ttgacaaaca
     2581 tgaaataaac cccttttaaa caaggggtaa tataacaaaa tattaccgtg attaaagtgc
     2641 tttgtcaata gaaatgccgg attcgacaga aaaataaaaa aaccggctta agagtagaag
     2701 atgactttag cagcatgaaa tccttcaatt ggtgcgagag gcgggacttg aacccgcacg
     2761 gttgcccact agatcctaag tctagcgcgt ctgcca
//
LOCUS       DASVMB010000158         7345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_387974,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000158 DASVMB010000000
VERSION     DASVMB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387974"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VF531_04810"
     CDS             <1..811
                     /locus_tag="VF531_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7713321.1"
                     /translation="TEIAAQTKQNALNANQANELALTAKQSAVQGDSRMKEMLQAMEE
                     INDSSANISKIIKVIDDIAFQTNILALNAAVEAARAGQHGKGFAVVAEEVRNLAARSA
                     NAAKETTALIEGSIKKVEAGTSIANETAQALNQIVEGITKAATLVGEIAAASNEQATG
                     IAQVNQGINQVAQVTQTNTATAEQSAAASEELSGQAEVLKNTVKRFKIKRIELGNLNG
                     LDSNVIKMLKEMADKKSSQGGSSQTGSEGRKEGSDFTKIRINLDDNEFGKY"
     gene            944..4213
                     /locus_tag="VF531_04815"
     CDS             944..4213
                     /locus_tag="VF531_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7713322.1"
                     /translation="MMSLKNGKNISLKVKLLSIVFAGMLVTPIIILINSILSLNQISV
                     TSQRIISDRLLTQVKEGLKASVESMVTSAETLYSNQAGIIPREELLEMIRSHFDAVKY
                     GDSGYYFAYLYDGTRIVAPENKSMEGQNLWNLTDKNGLKVVQGFIKAAQNGGDFLTYQ
                     WLNPQTQQEEEKLSYIAPLKLGDIELAIGTGTYLPMIKEANSDIIHSIDQTKLGKVRL
                     EVLISLTVMLLILLVLFVYISRNIINPITKLSDVAAKLSEGNLDIEIGQVTRKDELGK
                     MLLSFNKLVERVFWYESLLDAVPFPLSVTDNEMNWTFINKPVEDMLGIKRKAAIGQPC
                     SNWNANICRTEDCGIACLKRNKPQTFFDQQGRNFQVDSSYITNTKGQRIGHIEVVQDI
                     TEKTQGYEYQRIEVARLANNLHSLAKGDLNLDTEVSLGNEFTRNERENFIKINDNLVT
                     AKEAIKGLVSDANMLAQSALEGKLDVRADVTKHGGDFRKVIEGFNNTLDAVIGPLNVA
                     AEYVDRIAKGDIPPKITDSYHGDFNEIKNNLNMCIDAVKSMIDDVNMLTQAAVAGKLD
                     VRADAAKHGGDFRAIIEGVNQTLDAIVTPLNEANKILGQMELSDYTGAMTGQYQGMMK
                     RFAEMINSVRSHLLSVQDTMVRVSKGDTSRLEEFRKIGRRSENDRIMPAATAMMQAIE
                     DLINEVGKLSEACVKGNLRLRGNADHFEGGYRAIVEGFNQTLDAVIEPVNEANQVLQE
                     LAQGNLEVSVEGDYQGDHAKIKNSLNEAVRSFNEVLSQLNNAAEQVADNARHVSDSSQ
                     ALSQGSTEQAGTVEEITASMTELAAQTKQNALNANQANELALNAKQSAVQGDVRMKEM
                     LQAMGEINDSSANISKIIKVIDDIAFQTNILALNAAVEAARAGQQGKGFAVVAEEVRN
                     LAARSANAAKETTALIEGSIKKVAAGTGIANETAQALNQIVDGVTKAATLVGEIANAS
                     NQQATAIAQVNQGINQVAQVTQTNTATAEQSAAASEELSGQAEVLKNTVKRFKIKRIE
                     LGDLKGLDSGVLKMLQEMAAKQSPQGGNSGTSSGAEGRKAASNFTKIRINLDDNEFGK
                     Y"
     gene            4444..5265
                     /locus_tag="VF531_04820"
     CDS             4444..5265
                     /locus_tag="VF531_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX7713323.1"
                     /translation="MISLSDQEFQLLADYVKANYGINLAEKKLLVANRLQHVLMEKNF
                     NTFTDFFRYLLNDKTGEGVTTLVNRVTTNHTFFLREQEHFQFFQTRVLPELKKTAGQN
                     DLRIWSAGCSSGEEPYTLAMLIADFFGAQKVFWDTRILATDISQKVLEAAQRGIYPQD
                     QLTQIPQHWRKNHFRELDAERSQVVEGIRNEVIFRSFNLMNPVYPFKKRFQVIFCRNV
                     MIYFDAATRRELVRKFYDCTEPGGYLFIGHSESIRSEDTGYQYILPAVYRKDRST"
     gene            5262..6290
                     /locus_tag="VF531_04825"
     CDS             5262..6290
                     /locus_tag="VF531_04825"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX7713324.1"
                     /translation="MSIKQKIRVLVVDDSLVFREALCKGLAGDSAIEVVGTATDPFMA
                     KDCLTELRPQVMTLDVEMPKMNGIEFLQRLMPQYPIPVVVVSSASDRVFDALNAGAVD
                     FVTKPEHLTDHSFDAFINELIVKIKIASTAKVGFWKEEAPRHHHTGFSTAGNSDRIIA
                     IGASTGGTEAIAYILKELPTNLPGIVIVQHMPPVFTRMYAERLNNNCLLEVKEAQSGD
                     QVLPGRVLIAPGDQHMRLQKSGSGLAVECFPGEKVNGHCPSVDVLFQSVAEKMGNKAV
                     GVILTGMGYDGAKSLLEMRKHGAVTFGQDEASCVVYGMPKVAYEIGAVERQMSLQKIP
                     EALCQLFN"
     gene            complement(6316..6621)
                     /locus_tag="VF531_04830"
     CDS             complement(6316..6621)
                     /locus_tag="VF531_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX7713325.1"
                     /translation="MGRLRYLRYCFRYLFDTEVPFSKKIWILLVVLYFISPIDLVPDL
                     IPGLGFLDDLLILVLSLNKLLRVLEEYDLKIQNRKPGRESEASGVTIDEVEYKVHRD"
     gene            complement(6702..7025)
                     /locus_tag="VF531_04835"
     CDS             complement(6702..7025)
                     /locus_tag="VF531_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713326.1"
                     /translation="MKQNGRFCRFIDWIRRGFQKILPWKGINDQVSLKLFGLNMDLSR
                     RLPPDIPYELTVVVPRAEFRGKCNCPAGKNCEWEIILNSITIAHSPRERGKIGSQPPP
                     QIPRK"
     gene            7211..>7345
                     /locus_tag="VF531_04840"
     CDS             7211..>7345
                     /locus_tag="VF531_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7713327.1"
                     /translation="MLDRVVRIGLLYGFYGGLLTEKQRQAMELYYLENWSLAEIAGSE
                     G"
BASE COUNT         1984 a         1747 c         1965 g         1649 t
ORIGIN      
        1 gacggagatc gccgcccaga ccaagcagaa tgcattgaat gccaatcaag ccaatgagct
       61 ggccttaacc gcgaaacaat cggcggtcca gggcgattcc cggatgaagg agatgctcca
      121 ggcgatggag gagatcaacg actcttccgc caacatctcg aagatcatca aggtcattga
      181 cgacatcgcg tttcaaacca atatcctggc cttaaacgcc gcagtcgagg cggcccgggc
      241 cgggcagcac ggcaagggat ttgcggttgt ggccgaggag gtccgtaacc tggcggcccg
      301 tagcgccaat gcggccaagg aaaccactgc tttgatcgag ggttctatca aaaaagtgga
      361 agccgggaca agtatcgcca atgaaaccgc ccaggcgctc aaccagatcg tcgaagggat
      421 caccaaagcc gccacgctgg tgggcgagat cgccgccgct tccaacgaac aagccacagg
      481 aatcgcccag gtgaatcagg gtatcaatca ggtagcccag gttacccaga ccaacaccgc
      541 caccgcggag cagagcgccg cggccagtga ggaactgtcg ggccaggccg aagtattgaa
      601 gaacacggtc aagcggttca agattaaacg gatcgagctg ggaaatttga atggattaga
      661 ttccaacgtc atcaagatgt taaaggagat ggcggataag aagagttccc agggcgggag
      721 ctcccagact ggttccgaag gacggaaaga gggttccgat ttcacaaaaa tccggatcaa
      781 tctggatgat aacgagttcg gcaaatatta agtaaagtgg ggtaaccggc ggacgctttg
      841 atttgggatt cgagcgtccg tcctttttaa agctttgagt tttagttatc aatatcaatg
      901 cgttgattgg atcgaatcag attcagctac gacgggagtg aggatgatga gtttaaaaaa
      961 tggaaagaat atcagtttaa aggttaagct tttaagtatc gtattcgctg gaatgctggt
     1021 taccccaatt atcatactca ttaactcgat tttgtcattg aatcagatta gtgtcacctc
     1081 tcaaaggata atatccgata ggttgttaac ccaagtaaaa gagggactca aggcttctgt
     1141 tgagtccatg gtaacttcgg ccgaaacgtt gtattccaac caagccggta ttataccgcg
     1201 agaagaattg ctggagatga ttcgtagcca ttttgatgcg gtcaaatatg gagattctgg
     1261 ttactatttc gcgtaccttt atgatggaac ccgtattgtc gctccggaga acaagtcgat
     1321 ggagggtcaa aatttatgga acttgacgga taagaacgga ctcaaggtcg tgcagggatt
     1381 tatcaaggcc gcccaaaacg gcggcgattt ccttacttat caatggttga atccccagac
     1441 ccagcaggaa gaggagaagt tatcttacat tgcccctcta aaactgggtg acatagagct
     1501 ggcgataggt accggcactt accttcccat gatcaaggaa gccaatagcg atattatcca
     1561 tagtattgat caaacgaagc tcggcaaggt cagactggaa gtccttatta gtctgacggt
     1621 tatgttgttg atcctgctcg tgctctttgt ctatatctca agaaatatta tcaatccgat
     1681 tacgaaatta tccgacgtcg ccgcgaaact ctccgaggga aatttagata tcgagattgg
     1741 tcaagtaacc cgcaaagatg aacttggaaa aatgttgctt tcttttaata aattagtgga
     1801 gagggttttt tggtacgagt cattactcga tgcggttcct ttcccgcttt cggtaacgga
     1861 taatgagatg aattggactt ttatcaacaa gccggttgaa gatatgttgg ggattaagcg
     1921 taaggcggcc atcggtcaac cttgcagcaa ttggaacgcg aacatctgcc ggaccgaaga
     1981 ttgtggaatt gcctgtttaa aacggaataa gccgcaaact ttctttgatc aacaaggccg
     2041 taatttccaa gtagattctt cctatatcac caataccaaa ggtcagcgta tcggtcatat
     2101 cgaagtggtt caggatatta cggaaaagac tcaaggatac gaatatcaac ggattgaagt
     2161 cgccagactc gcaaacaacc tgcactccct ggctaaagga gatctgaatc tggacacgga
     2221 ggtctccctg ggcaacgagt ttaccaggaa cgaacgggaa aacttcatca agatcaacga
     2281 taacctggtt actgcgaaag aagcgatcaa aggtttagta tccgatgcca atatgctggc
     2341 gcaatcagct ttagagggca aactggatgt ccgagccgac gttaccaagc acggcggtga
     2401 tttccgtaag gttatcgaag ggtttaacaa cacgctggat gcggtgatcg ggccgttgaa
     2461 cgtagccgcg gagtacgttg accggatagc caagggcgac atcccgccca agatcaccga
     2521 tagctatcac ggcgatttta atgagatcaa gaataatctc aacatgtgca tcgacgcggt
     2581 gaagtctatg attgacgatg tcaacatgct gacccaagcg gcagtcgccg gcaaactgga
     2641 tgtccgggcc gacgcggcta aacacggcgg cgacttccgg gcgatcatcg agggagttaa
     2701 ccagaccctg gatgccatcg tcaccccgct caacgaggcc aataagatat tggggcagat
     2761 ggagttgagc gattacaccg gggcgatgac cggccaatac cagggcatga tgaagcggtt
     2821 tgccgagatg attaattcag tccgctcgca tttgttaagc gtccaagata caatggtcag
     2881 ggtatcaaaa ggtgatacca gccgtctcga agagttccgc aagattggaa gacgttcgga
     2941 gaacgaccgg atcatgcccg ccgccacggc catgatgcag gcgatcgagg acctcatcaa
     3001 tgaggtaggg aaactcagtg aagcctgtgt caagggtaac ttacgcctcc gaggtaacgc
     3061 cgatcatttc gagggcggtt accgggcgat tgtggaagga ttcaaccaga ctctggatgc
     3121 ggtgattgag ccggtaaacg aagccaacca ggtcttgcag gagttagccc aggggaacct
     3181 tgaggtgagc gtggaaggag attaccaggg agatcacgcc aagatcaaga actccctgaa
     3241 tgaagcggtc cggtcgttca atgaagtttt gagccagtta aataatgccg ccgagcaagt
     3301 ggcggataac gccagacatg tctccgactc cagccaggca ttgtctcagg gctccaccga
     3361 acaggcggga accgtcgagg agattaccgc ctcaatgacg gagctcgccg cccagaccaa
     3421 gcagaatgcg ttgaacgcca atcaagccaa tgagttggcc ttgaatgcca agcaatcggc
     3481 ggtccagggc gatgtccgaa tgaaggagat gctccaggcg atgggggaga tcaacgactc
     3541 ttccgccaac atctcgaaga tcatcaaggt cattgacgac atcgcatttc aaaccaatat
     3601 cctggcctta aacgccgcgg tcgaggcggc cagggccgga cagcagggta aaggatttgc
     3661 ggttgtggcc gaggaggtcc gtaatctggc ggcccgcagc gccaatgcag ccaaggagac
     3721 caccgccctg atcgaagggt ctatcaaaaa agtggcggcc ggtaccggta ttgccaatga
     3781 aaccgcccag gcgctcaacc aaatcgtcga cggtgtaact aaagccgcga cgttggtggg
     3841 tgagatcgcg aatgcttcca atcaacaagc cacagcaatc gctcaggtga atcagggcat
     3901 caatcaggtg gcccaggtta cccagaccaa taccgccacc gcggagcaga gcgccgcggc
     3961 cagtgaggaa ctgtcgggcc aggccgaggt attgaagaac acagtcaaac ggttcaagat
     4021 caaacggatt gagctgggag atctaaaagg attagattcc ggcgtcctca aaatgttgca
     4081 ggagatggcg gctaagcaga gtccccaggg agggaactcc ggaaccagca gcggtgctga
     4141 aggtcggaaa gcggcttcca atttcacaaa aatccggatc aatttggatg ataacgagtt
     4201 cggcaagtat taagacaatg cccaaggact agacgctgga acaggtaatc ggcggacgcc
     4261 cttactttaa gtttcgggcg cccgcttttt ttaccttttg gggttaagat tggagcgacg
     4321 gatgccgatt tatttattgg acgctgctgt tcggaacccc aatcgatgat tcgctaacat
     4381 ccactcataa aaaaagggtc ttcgaatcta acctgacggg catcataaga taggagcgag
     4441 ccaatgatca gcttatccga tcaagagttc cagctgttgg ccgattatgt aaaggccaat
     4501 tacgggatca atctggccga aaagaaacta ttggtggcca atcgcctcca acacgtgctg
     4561 atggagaaga acttcaacac attcacggat ttctttcgat atctgttgaa cgataaaacc
     4621 ggggaaggcg tcacgaccct ggtcaaccgg gttaccacca accatacgtt ctttctgcgg
     4681 gaacaggaac attttcaatt tttccaaacc cgggtgttgc cggaattgaa aaaaacggcg
     4741 ggtcagaatg atctacggat ttggagcgcc gggtgttcca gcggtgagga gccgtacacc
     4801 ttggcaatgc tgattgccga tttcttcggc gcccaaaagg tgttctggga tacccggatc
     4861 ctggccaccg atatctccca gaaagtcctg gaggcggctc aaagaggcat ttatccacag
     4921 gatcagctga ctcagatccc tcagcactgg cggaaaaacc acttccgaga gctggatgcg
     4981 gaacgcagtc aagttgtgga agggatccgt aatgaagtga tctttcgcag tttcaacctg
     5041 atgaacccgg tgtatccgtt caaaaaaaga tttcaggtca ttttctgccg gaacgtgatg
     5101 atttattttg atgcggccac ccgtcgggaa ttggtgcgaa aattttacga ttgtaccgaa
     5161 cccggggggt acctttttat cggtcactcc gaatcgatcc gttccgagga taccggctac
     5221 cagtacatat tgcctgccgt atatagaaag gacagatcaa cttgagtatc aaacagaaga
     5281 ttagggtttt agttgtcgac gactctctgg tgttccgcga agctctttgc aaagggttgg
     5341 ccggagattc cgccatagaa gtggtgggta ctgccacaga cccttttatg gccaaggatt
     5401 gccttactga actacgaccc caggtcatga cgctggacgt agagatgccc aagatgaacg
     5461 ggattgaatt tttacagcgg ctgatgcctc aataccctat tccagtagtc gtagtaagct
     5521 ccgccagtga tcgggtattt gacgctttga acgccggtgc cgtcgacttt gtgactaaac
     5581 ccgaacattt gacggatcac agttttgacg ccttcatcaa tgagttgatc gtcaagatca
     5641 agatcgcttc aaccgccaaa gtgggtttct ggaaggaaga agcgccgcgg caccatcata
     5701 ccggtttttc cactgcgggc aacagcgata gaatcatcgc catcggtgca tccaccggag
     5761 ggaccgaggc gattgcatat atcttaaaag agctgccgac gaatcttccg gggatcgtca
     5821 tcgtacagca tatgccgccg gtgttcacca ggatgtatgc ggaaaggttg aacaacaatt
     5881 gtttgcttga ggttaaggag gcgcaaagcg gcgatcaagt cctaccgggc cgggtactca
     5941 ttgcccccgg cgatcaacat atgcggctgc aaaagtccgg ttcaggtctg gctgttgaat
     6001 gttttcccgg ggagaaagtg aatggccatt gtccctcggt ggatgtactg ttccaatcgg
     6061 tggcggaaaa gatgggaaac aaagcagttg gagtcatcct aacgggcatg ggctatgacg
     6121 gcgccaaaag cttgttagaa atgaggaaac acggcgcagt taccttcggc caggacgagg
     6181 catcctgtgt ggtttacggt atgcccaagg tggcctacga gatcggagcc gtagaaagac
     6241 aaatgtccct gcagaagatc cccgaagccc tttgtcaatt gttcaactag agcaaattgg
     6301 attaatactc ccgtctcaat ctcggtgaac cttatactca acttcatcga tggttacccc
     6361 ggaagcttcg gattcccgcc cgggcttccg gttttggatc tttagatcat actcttccaa
     6421 gacccggagc aatttattta aggaaagaac caggattagc agatcatcca aaaaaccgag
     6481 gccgggtatg aggtccggaa ccaggtcgat cggggatata aaatagagca ccaccaggag
     6541 gatccaaatt ttttttgaga aaggaacttc cgtgtcgaac aaatacctga aacaataacg
     6601 gagatatcta agccgtccca tagcaatcac cccggtttat tgtatcacgt ccggtacgga
     6661 caagcaaatc aggcacgccg atcgcggtca ttccccaaaa atcatttacg ggggatttgg
     6721 ggtgggggct gtgacccaat ttttcccctc tcccggggtg agtgtgcgat agtgatgctg
     6781 ttaaggatga tttcccactc acagttttta cctgccggac agttgcattt ccctcgaaat
     6841 tcggccctgg gtaccactac tgtcagttcg taggggatat ccgggggcaa tctcctggag
     6901 agatccatat ttagaccgaa gagttttaaa ctcacctggt cgttaatgcc tttccatggc
     6961 agaatttttt gaaagccacg ccggatccag tctatgaacc ggcaaaatcg gccgttttgt
     7021 ttcatccgtc atcccaccca ttgaagcatc ttatgaatga atccctcaaa atgtcgcttg
     7081 tattcacaac ccccggtata ctctcaagag aaaaagcttg acaacccaag tcactccgga
     7141 tatactatca ctgttaagct tatttacttg acaccatcga ttggaggatc caacggtggc
     7201 tgatgcggat atgctggatc gggtggtccg aatcggactg ctctatggtt tttacggtgg
     7261 acttttaaca gaaaaacagc gccaggcaat ggagctttac tatttggaaa actggtcttt
     7321 agcggaaatt gccgggtccg agggc
//
LOCUS       DASVMB010000159         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_683709,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000159 DASVMB010000000
VERSION     DASVMB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683709"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..779)
                     /locus_tag="VF531_04845"
     CDS             complement(168..779)
                     /locus_tag="VF531_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7713328.1"
                     /translation="MPHRSGGQFAGYDFTFFWMMGGITAAPLFEPKISFLNVIAIIAI
                     IYLLHYLLSFLMIKSRTLAKLITGEPIPLVSGGKIIRRNMAKALFPLELLFSELPSMN
                     APNIHEVQEAVLETNGHVSVVRKGEALPVTPAELNIPTAHIHSGLPIILIHDGFIQPQ
                     NLKKIGRNQEWFEQELSKNGVLNLKDVYYAQIDAAGTLYVSVK"
     gene            complement(900..1601)
                     /locus_tag="VF531_04850"
     CDS             complement(900..1601)
                     /locus_tag="VF531_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7713329.1"
                     /translation="MGFLKGFLHGAKDLPFWILAIRAVFLYIALIIATRLMGYRQVGI
                     LTGHNYLVAAGIVSLAAIRMISPESSFVSGVVIIFCYAGVNILLSYLDLKLPKLVDRL
                     PVVLMRGGILDRRMLSDAHITLENLLGQLRLKEASDLTEIDTLVLEPTGKLSVIKKPK
                     YLPVTRQMMKLPAKNSGLPVALIFDGQIQTENLVRLGHDRRWLEQELAKQGFSSPKSV
                     FLAMLNPDGTLYTSA"
     gene            1661..>1932
                     /locus_tag="VF531_04855"
     CDS             1661..>1932
                     /locus_tag="VF531_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713330.1"
                     /translation="MTFEAHGIATGLGGSPGGFVFLWGFIGFFLFVRRLFADFLAKLH
                     RTGIDEMIGKVIPIAGDIVFDPDLTCGAILAFALEFATFHGAFNFS"
BASE COUNT          509 a          495 c          405 g          523 t
ORIGIN      
        1 ccggcttgac tgctccatcc ctttcagcat ctgctgcaat tgcgggtcgg taatttgatt
       61 ggtcaggccg gcatactttt tagccatttg ttcctcggca aacagcagtt ctttaaaagc
      121 gttattcatt cgttcctgct cggtaagcat gatcttaacc tccttattta tttaactgag
      181 acatacaaag tcccggctgc atcgatctgg gcatagtaga catcctttaa attgagaacg
      241 ccgtttttac ttagttcttg ctcaaaccat tcctgattcc gtcctatttt tttcaagttt
      301 tgtggctgaa tgaaaccgtc gtgaattaaa atgattggta atccgctatg gatatgggct
      361 gtcggtatgt tcaattcggc aggagtaacc ggtaaagcct ctccttttct gaccacactg
      421 acatggccgt tggtctccaa aaccgcttct tgtacctcat gaatattggg ggcattcatg
      481 ctcggaagtt cagagaagag cagttccaat gggaataatg ctttcgccat attccggcga
      541 ataatcttcc cgccggacac cagtggaatc ggttcaccag taattagctt agccaaggtc
      601 cggcttttga tcatcaaaaa tgataacaga taatgcagaa gataaatgat cgcaatgatg
      661 gcgatgacat ttaagaaact gattttcggc tcaaaaagcg gcgcggcggt aataccgccc
      721 atcatccaga aaaacgtaaa atcataacct gcgaattgac cgccggatcg gtgcggcaat
      781 attttcccct ccgcccataa caagatacct acgaggacag ttctgaggac aaacccccaa
      841 ggacttaact ctgtggccga tcctagaaca ttttttaaaa aatccaccgc cttcctcctt
      901 taagcgctgg tataaagggt tccatcggga ttcaacattg ccagaaaaac actcttgggc
      961 gagctgaacc cttgcttcgc caattcctgt tccaaccatc gccggtcgtg gcccaaccgg
     1021 actaaatttt cggtttgaat ttgtccatca aagattaatg ctaccggcag cccggaattt
     1081 ttggcaggga gtttcatcat ttgccgggtt accggtaaat acttcggttt cttaattaca
     1141 gataatttgc cggtgggttc caggaccagc gtgtcaatct ccgtaagatc ggaggcttct
     1201 ttcaagcgta actgccccag caggttctcc aaagtaatat gcgcatcaga cagcatccgg
     1261 cggtccaaga tcccgccccg catcaaaacc acaggcaacc ggtcaaccaa tttcggaagt
     1321 ttcagatcca aataagataa gagtatattg actcctgcat aacaaaaaat aatcaccact
     1381 cccgagacaa atgatgattc aggactaatc atccggatag ccgccaaact cacgataccg
     1441 gcagccacca aataattgtg accggttaaa atccctactt gcctgtagcc catcaaccgg
     1501 gtagcaataa tcaaagcaat atacaaaaaa accgcccgga tcgccaatat ccaaaatggc
     1561 aagtccttgg ctccgtgaag aaagccttta aggaacccca tccgccctca gcctttcaac
     1621 ccatgaatta tttttactcg caacaacaac cacccttgtt ttgacgtttg aggcgcatgg
     1681 tatcgcaaca ggtttggggg gaagtccggg cggtttcgtt ttcctgtggg ggttcattgg
     1741 tttctttctt tttgtccgcc ggcttttcgc cgatttcctc gctaaactcc accgaactgg
     1801 cattgatgaa atgattggca aagtcatccc gatcgccggt gatatcgttt ttgacccaga
     1861 tcttacctgc ggtgcaattt tggccttcgc cctagaattc gcaactttcc acggtgcatt
     1921 taactttagt aa
//
LOCUS       DASVMB010000160         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_166873,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000160 DASVMB010000000
VERSION     DASVMB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166873"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF531_04860"
     CDS             complement(<1..265)
                     /locus_tag="VF531_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713331.1"
                     /translation="MESLLWEYKISTDKDLISVDRVSDLLSKSYWANQRGKEKIKESI
                     KNSVCVGIYDPEKMIGFARIVTDHATMYWLCDVIIDEAYRGNGL"
     gene            complement(389..1516)
                     /locus_tag="VF531_04865"
     CDS             complement(389..1516)
                     /locus_tag="VF531_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEX7713332.1"
                     /translation="MSKGAFYTGKYPNLLAEIGISQDKIDEKIKNTFNQMFFDPEERI
                     YFESGKDMGYMLDTGNNDARTEGMSYGMMMAVQMDRQDIFDRLWLFSKTFMYQSSGKY
                     QGYFAWSVRVDGTKNAEGPAPDGEEYFAMALFFASKRWGDGKQPFDYSVQARDILRHC
                     IHQSEFVEGGEPMWDSGNHYIKFVPEAPFSDPSYHLPHFYELFALRADEQDRSFWQQA
                     AEASRHYLHISCDKDTGMAPEYAEFDGTPKRLFRDCQFYSDAYRVAMNIGLDAAWFNK
                     DEALGEIVDKLQAYFSEKTTLGEYHSYTIKGEPFEEPAMHPVAIIATNAAGSLAAKGK
                     YRLQWVKDFWELPLRKGVRRYYDNCLYFFSLLMLAGEYKIY"
     gene            complement(1551..>1801)
                     /locus_tag="VF531_04870"
     CDS             complement(1551..>1801)
                     /locus_tag="VF531_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713333.1"
                     /translation="CSSKENLTTLQYEMMKLLSSAMNSATDRSAASVFGGNQLINTVT
                     PVSMRAAITVVASVPILFVYPFLQKYFVNGVTIGAVKG"
BASE COUNT          476 a          463 c          344 g          518 t
ORIGIN      
        1 ccaatccatt tccccgatat gcttcatcaa tgattacatc acacagccaa tacatcgtgg
       61 cgtgatcagt aacaattcgc gcgaaaccaa tcattttctc cggatcatat atcccgacac
      121 agacggaatt cttgattgac tctttaatct tctctttacc tcgttggttt gcccaataac
      181 ttttacttaa caaatcagaa actctatcca cggaaatcaa gtctttatcg gtacttattt
      241 tatattccca taataatgac tccattttgt tcccctcctt attaatcgga ccggatagcc
      301 atatggctat cttgctacat actacggatt ggcgataaga tcattattga tatcgtccca
      361 ttcaactctt cccccgagga tctttttttt aataaatctt atactctccc gccaacatga
      421 gcagggagaa aaagtacagg cagttgtcgt aatatctgcg aactcctttt ctgagtggaa
      481 gctcccagaa atccttgacc cactgaagtc tgtatttccc ctttgctgcc agtgaaccgg
      541 cagcattggt tgcaatgatg gccacgggat gcatggcggg ctcttcaaaa ggttcaccct
      601 tgatagtgta ggaatgatac tctcccaaag tagttttttc gctaaaataa gcttgaagct
      661 tatctacgat ctccccgagt gcttcatcct tattgaacca cgccgcgtcc aacccgatat
      721 tcatggcgac acgatatgca tccgagtaga attgacaatc acggaacaat ctttttggag
      781 taccgtcaaa ttcggcatat tcgggtgcca taccggtatc cttgtcacag gaaatatgca
      841 ggtaatgtcg gcttgcctcc gcagcttgtt gccagaagga tctatcctgt tcatcggccc
      901 tcagcgcaaa aagttcataa aagtgtggca gatgatagga gggatccgaa aatggcgctt
      961 ccggtacgaa ttttatgtag tgattgccgc tatcccacat cggctctccg ccttcgacaa
     1021 attcggattg atgaatacag tgccgtaata tatccctggc ctgtacacta tagtcaaaag
     1081 gttgcttacc gtcaccccat cttttgctgg caaaaaagag cgccatcgca aagtattctt
     1141 caccgtccgg cgccggaccc tcggcgtttt ttgtcccgtc gacccggacc gaccaggcaa
     1201 aataaccctg gtatttcccg ctgctttgat acataaaggt cttggaaaat aaccataacc
     1261 tgtcaaagat atcttggcga tccatttgga cggccatcat cattccataa ctcataccct
     1321 cggtacgggc gtcgttattt cccgtatcca gcatatagcc catgtctttg cccgattcaa
     1381 aataaattcg ctcttccgga tcaaagaaca tttgattgaa cgtattctta attttctcat
     1441 ctattttatc ttgggaaata ccgatctccg ccaacagatt tgggtatttt ccggtataaa
     1501 aagctccttt ggacatgatt ttttcccctt ttctatgacg ttgtatatgc tcagcccttt
     1561 actgcgccga tggtcacacc gttaacaaaa tacttttgca gaaacggata aacgaacaaa
     1621 ataggcactg aagcaaccac cgtgattgcc gcgcgcatcg aaaccggcgt caccgtatta
     1681 ataagctgat taccgccgaa taccgacgct gcggatcgat ccgtagccga attcattgcc
     1741 gaggacaata atttcatcat ttcatattgg agcgtggtca aattctcctt actcgaacag
     1801 a
//
LOCUS       DASVMB010000161         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_536738,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000161 DASVMB010000000
VERSION     DASVMB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536738"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VF531_04875"
     CDS             complement(<1..1483)
                     /locus_tag="VF531_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX7713334.1"
                     /translation="MKLKNRLHFTAGMILLAVMGVIIFSLPVLADTNPNVVLFEVQGN
                     AHIPTARILGVISNTKIGEPLDPKNVQLDMQAISGLGYFADVQAKTESILGGVKLIFT
                     VVENPVFKELQISGTTQIKPEELLPYFTQKPGEIFNTTVFREDLGKALKFCSDQKGFY
                     VQPKNGKLDISPSGVVRLGLVELKYGSIKVQGLVKTKDLVVLRELSFKEGDIIDINAF
                     KDDWARLMRLRLFNSINPEFQPAADPEKLNLTLNIQEAQVGTISGGVSYVPGSGEIGG
                     MLSYSDPNLMGRGQNLSLDVNLAPSSNNVTFSFYEPWLDASHTSFGMSLGNTETTMSS
                     TMTNWFPDSKDVYTMNFLRTGLGLWFDRPISTSTTGRVRLNFEKNDIASYQPAGLGAP
                     DKPMTFWDNSLQLQLFQNKLQYQDAFFVKGGYYLSGSYNFAGKMLGGAYDYQKVNLEG
                     KAFHQITSDLVLGTHLEGNYLTGSYPDYDLLYLGGMRQLRGYSD"
     gene            complement(1510..2235)
                     /locus_tag="VF531_04880"
     CDS             complement(1510..2235)
                     /locus_tag="VF531_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713335.1"
                     /translation="MPQQEGEPRMIETDIGKRCDSGNLLQHIDDAKVWLEKAKTEYKQ
                     ANPLQGELILNLAQAEVKYAWELSRKRCVAKDKGDKNGSRLKFYLPLAASFVLISGVF
                     LWQFGHKGPNSGGTLTARNLKPGVTAGTPLPGTASPAVTSVAPGTPPPVLPQTQSDKV
                     AVNEGTVKEPADTTEPDRISQTLTSATGSKPVRQPDTVVKTRNQIPDTANQVQSNLQP
                     VTEFSIDEEALTKEASRSLRSGK"
     gene            complement(2557..3201)
                     /locus_tag="VF531_04885"
     CDS             complement(2557..3201)
                     /locus_tag="VF531_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7713336.1"
                     /translation="MIHQYLAELAKIQLLMPAEEHLLWKQFKNENSRDARYRLIESYQ
                     PLVYKIASRLTDREDLFLDLLQEGTIGLIEAVENFNPQRAVKFSTFAPHRIRGRMIDF
                     LKRQHRAHDSLEVALKEEELAFFLAQVADEHVDIEEEVSVKLLGGQVKEAIARLSPKE
                     QKVIQDLYLLDKEPVTSAREMNVSLSYLYKLQKKALQRLRGMLSKLRAELKIND"
     gene            complement(3333..>4319)
                     /locus_tag="VF531_04890"
     CDS             complement(3333..>4319)
                     /locus_tag="VF531_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX7713337.1"
                     /translation="KGVYGPGRFSLNGGADFSDLKQLKLNMTLSGTKMFYKNQFFDGY
                     GDLNLQLTGTPSDSLISGDITIYNCKAGLLGINSSAAAEVGWNPRLDLAIKTGSNVRG
                     RFVGLADVRVRGAVRVKGVLNNPSLEGQLSSNSGILTFYNQTFRVNTGKAIFKQEQGY
                     IPYIEVSSSLKRPTIEFFLDIQGMLDNINISLTSQPYLSQADIFSMLNWETLRGEEPL
                     APKDLLTNNLSSVTDTVLGDFLYQVRQTLNIDYLYLEPDKQMNNFRLSIGNYVTDQLF
                     ISYSSRVFNQKPLDSWSLDYHFDPHLSLGYSYTLDENNQGEAYWQLMYQIRF"
BASE COUNT         1108 a         1089 c          976 g         1146 t
ORIGIN      
        1 aatcgctata tccccgcaac tgacgcatac cgcccagata aagcagatca tagtccggat
       61 aagacccggt caaataattg ccttccaaat gagtaccgag cactaaatcg gaggtaatct
      121 gatgaaaagc cttcccttcc aggttgactt tctgatagtc ataagcgccg cccaacatct
      181 tcccggcgaa attataacta ccggagagat aataaccgcc ctttacaaag aaggcgtctt
      241 gatactggag tttattctgg aataactgca attgcagtga attatcccag aaagtcatcg
      301 gtttgtcggg tgctcccaga ccggccggtt ggtaggaggc gatatcattc ttctcgaaat
      361 tgagcctgac ccgaccggtg gtgcttgtag agatcggtcg atcgaaccag agcccgagac
      421 cggtccggag aaagttcata gtataaacat ctttggagtc cggaaaccaa ttggtcatgg
      481 tggaactcat cgtcgtctcg gtattgccca aagacatccc gaaggaggta tggctggcgt
      541 cgagccacgg ctcataaaac gagaaagtaa cgttattact gctgggtgcc agattgacat
      601 ccagtgatag attctgcccc cgtcccatca aattcggatc ggagtaggat aacatcccgc
      661 cgatctcccc actgccggga acataggaaa cgccgccgga gatggttccg acctgagctt
      721 cttgaatgtt caaagtgaga ttaagtttct cgggatcggc cgccggctgg aactccgggt
      781 tgatgctatt aaacagacgg agccgcatca atcgggccca gtcatccttg aaagcattga
      841 tgtcgatgat atccccctct ttgaagctta actcccgcag aactacgagg tctttggttt
      901 tgaccaaacc ctgaacttta atggagccgt actttaattc gaccaaccct aagcggacca
      961 ctccgctcgg acttatatca agcttcccgt ttttgggttg aacgtaaaat cccttttgat
     1021 cactacaaaa tttcagcgcc ttccccagat cctcccggaa aacggtggtg ttaaatatct
     1081 ctcccggctt ctgggtaaag tagggaagca actcttcggg tttgatttga gtagttccgg
     1141 agatctgtaa ctctttgaat accggatttt caaccactgt gaagatcagt ttcactccac
     1201 ccaggatcga ctcggtcttt gcctgaacat cggcgaaata acccaaacca cttatagcct
     1261 gcatatccag ttgcacgttc ttgggatcca aaggctcgcc gatcttagta ttggagatga
     1321 cccctaaaat tcgggctgtt gggatatgcg catttccttg gacttcgaaa agcaccacat
     1381 tgggatttgt atccgccagc accggcaggg agaagattat gactcccatt acggccagaa
     1441 gtatcatccc agcggtgaaa tgaaggcggt tcttgagttt caaccacatc accctttttt
     1501 attaattcct tactttccac ttctcaaact acgcgaagct tctttggtca aagcctcttc
     1561 gtcaatggaa aactccgtaa ccggttgcaa gttggactga acttgatttg cggtatcagg
     1621 aatttggttc cgggtcttga ccactgtatc cggttgccga accggtttgg aaccggtggc
     1681 ggacgtaagc gtttgggata tccgatctgg ctcggttgta tcagccggct ccttcacggt
     1741 cccctcgttc acggctacct tatcggactg agtctgtggc aaaacagggg gcggtgttcc
     1801 aggcgccaca gatgtcactg ccggggaagc tgttcccggt agcggagttc cggcggtgac
     1861 ccccggcttt aaattccggg ctgtcaaagt cccaccggaa ttcggtcctt tatggccgaa
     1921 ctgccatagg aaaacacccg aaatcaacac aaaagaagcg gctaacggta gataaaactt
     1981 caaccgggat ccgtttttgt cgcctttatc ctttgcaacg catcgtttac gacttagttc
     2041 ccaggcatat ttaacctcag cctgagccag attgaggatc aattcaccct gtaaaggatt
     2101 cgcctgttta tactcagttt ttgccttttc caaccatact ttggcatcgt cgatatgctg
     2161 cagcaaatta ccggaatcgc accgtttacc gatgtcagtc tcaatcatcc ggggttctcc
     2221 ctcttgttgt ggcatatgga tcacaccttt ctttcaatac ggcggtaacc ccgccaatga
     2281 agaaatttca gaatcgttct cctcatggca agatcctccc gagcatttta ttaccctcga
     2341 cgatccataa tatctgcaag ttggaactgt aaatgtccga tggttttacg ccgattttcc
     2401 gtctctactc gcaatttttg atgtttacac cggttttaat acctacataa ccccctattt
     2461 ccgaaaaaaa gaccttccgg taagtccgaa gcttccaagg gttgaaaaag atcattatga
     2521 tctgatccgc aacgattttt tcgggaaaag gaattgctag tcattgattt tgagttcggc
     2581 gcgaagcttg gacaacatac cccggagtcg ctgcagggcc tttttttgta acttgtaaag
     2641 gtacgacaag ctgacattca tctcccgggc ggaggtcaca ggttctttat caagcaagta
     2701 aagatcttga atcacctttt gctccttggg actgagccgg gcaatcgcct ctttgacttg
     2761 tccgcccagg agtttcacgg agacctcttc ctcgatatcg acgtgttcat cggctacttg
     2821 agccaaaaag aaagcaagtt cttcctcttt aagcgcgact tcaagcgaat cgtgggcccg
     2881 atgctgccgc ttcaaaaaat cgatcatccg gccccggatg cgatggggcg caaaggtaga
     2941 aaatttcacg gctctctggg gattaaagtt ctccactgct tcgatcaggc cgatggtgcc
     3001 ctcctggaga agatccagaa ataaatcttc acgatccgtg agccgggaag caatcttata
     3061 aaccaggggc tgataggatt cgatcaaacg gtacctggca tcacgcgagt tttcgttttt
     3121 aaactgcttc cacagcaaat gttcttcggc cggcataagc agttgaatct tggccagttc
     3181 tgcaaggtat tggtgaatca accctccacc ttcccggaag aaacttcggt aaactttcgg
     3241 actaaagttc tcgttttctc attgatatcc tgccatacag gttaatttct ttcaggaaaa
     3301 cctgggtttt tttagaattt caatctgttc tgctagaatc ggatctgata catcaattgc
     3361 caataggctt ctccttgatt gttctcatcc aatgtgtagg agtatcccag agaaagatgg
     3421 ggatcgaaat gataatcaag gctccagctg tctagaggtt tttgattaaa aaccctactg
     3481 gaataggaga tgaataattg atccgtgaca taatttccga tgctgagccg gaagttgttc
     3541 atctgtttat cgggctcaag atacaaataa tcaatgttca aggtctgacg cacctgataa
     3601 agaaaatcac ccaaaaccgt atccgtcaca gaactcagat tattcgttaa aagatccttc
     3661 ggcgctagcg gttcttcacc acggagcgtc tcccagttga gcatcgaaaa aatatcggcc
     3721 tgtgacagat agggttgaga agttagacta atattgatgt tatcgagcat gccttgaata
     3781 tccaagaaaa actcaatcgt cggccgcttt agactggaac tcacctcgat atacggtata
     3841 taaccctgtt cctgcttaaa aatggctttg ccggtattca cccgaaaggt ttgattatag
     3901 aaagttaaga taccactgtt cgagctgagt tgaccttcaa gggacgggtt attcaacact
     3961 ccttttaccc ggactgcgcc acgaacccgt acatcagcca atcccacaaa acgtccccga
     4021 acattactac cggttttgat ggccaaatca agccggggat tccagccaac ctccgccgcc
     4081 gcgcttgaat tgatccccaa tagtccggcc ttacaattat agatggtgat gtcaccggaa
     4141 atcaagctat cactgggagt cccggtaagt tgcaagttaa ggtccccgta cccgtcaaag
     4201 aactggttct tgtaaaacat cttggttccg gataaggtca tattcaactt aagttgtttg
     4261 agatcgctga aatccgctcc gccgttaagg ctgaaacgac cgggtccgta cactccctt
//
LOCUS       DASVMB010000162         6393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_21050,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000162 DASVMB010000000
VERSION     DASVMB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21050"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF531_04895"
     CDS             complement(<1..466)
                     /locus_tag="VF531_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7713338.1"
                     /translation="MSLGPSLEQKIQSLQKDNPYGRCVWRCDNEVVDHQTVEIEFEDG
                     SIATHSMNGNSAKPCRYIHIIGTTGEIEGVMEEGYFIIRRPDARKGHEYSEAKVNVNV
                     VNDMHGGGDLHLVADFVNVVCGKPPSISTTSLEDSINGHLIGFAANEANEERK"
     gene            complement(565..1023)
                     /locus_tag="VF531_04900"
     CDS             complement(565..1023)
                     /locus_tag="VF531_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7713339.1"
                     /translation="MKGKISETTLPLLSAGYDVLLEKPIGVNREEVLKLLAAARNTNR
                     TVMICHVLRHAPFYAGIRKRISAGEIGEILSINTQENVSYHHMAVSYVRGKWNNPRVC
                     KSTMLMAKSCHDLDLITWMKSNIRPRAVSSLAVLCILHRTRPLPVRGPGV"
     gene            complement(1043..2866)
                     /locus_tag="VF531_04905"
     CDS             complement(1043..2866)
                     /locus_tag="VF531_04905"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX7713340.1"
                     /translation="MEKILTVPDTEKEYRKYLATGNEYLSIPSILGNGEIETMNVILD
                     RTRSLIDFSGKYLVKPQIRIHNRSLTLTAKKSWYEKSWIPSFEYEGSGLWIMVRIISP
                     KGFKGLVYGITVKNITNAELDITCGVEGIWAESSSTVFHTREIFAKKCISWHRWTHSL
                     ILELKDSLTLVALGISGDSDYELDYDQKAMTYAVTNRTRLKSNEEKVFYYYYSVNMEP
                     DGAGAVNIDLRRRGGESLYADELRWLGEHRVEPDDPSLENRMTLEEKMNRNLLFSYFF
                     ATGYALDTEEQVLLTSRSPKYYVSAAFWARDSLLWSFPAICIIDHQEAKKVLQTCFTR
                     YLKNAGIHSLYTNGSVLYPGFELDELAAYVIALEEYIEWTHDKNILDAEEIQRGLHHL
                     VSELERRHSGEDCLYRTELDPSDDPISYPYLIYDNVLVWKALVFLSQNGYVSPKAADE
                     LRIAIEKHGIAEGVFGPMYAWSTDCGGHHEIYDNPPGSLVLLAYYGFCSRDDEVYRNT
                     LKWIYSEHNKHRYQSPRFDSTGCIHTPFPWLISICNYLLVFHDESRLRMIEELELDNG
                     FACESFDSESGIVKTGGAFATCAGFLGYTLARLLKNHGIHG"
     gene            complement(2912..5032)
                     /locus_tag="VF531_04910"
     CDS             complement(2912..5032)
                     /locus_tag="VF531_04910"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HEX7713341.1"
                     /translation="MDSDFGTGNNLFEITFSSTHGGITGLLNKTTGDNYVKKKAAGLN
                     MPFALFYLDHHCRKRRLVPRVLSGCESTEDGRVISLKFQSMVNEEGQEWAVQAEMTVF
                     CFPDDAESIWRLSIKNHTENLKIVEVLFPYISGIWIGDTHQDDFLVYPHHAGEKIQNP
                     AENYASQRYLQFDRAGSKREGSYYSREINYCGLASMMWMDYYDDSDGGLYLASYDDDY
                     LLTGVRVEVGGPADPWVGFSFRKYLPIEPGTAWTSKPYSVGIHRGDWHWGADRYRAWF
                     NKGRVYPEFAEDLLTQSVLCPRYDFRRNEGIFHKFKEIPAMYDEARADGINHFLIAGW
                     NRQGFDSNYPEYFPDMELGSPLDLARGVEYINAHGGMVTFYVNVRIFDIHSDYFPTLG
                     TEWALKDEQGKWIKETYGPPSFAVMCPKDKAWQQRVVDYATVLVKWYGAKGIYLDQLG
                     SATPYPCYDQAHNHEHHGLFNQGYLEMIRGILEAADPDSPPFLMIENCGDIYGEYIFA
                     NLTWNGTFYDEFFNLYKYTFPHYIQINMVNPRRIPERRLRHQWFYRDVERAMLLGSIL
                     WVELGERFVAEDDCLRSYLRNVIKMRQACAYYFAHCTFRDDLEIRYDPRIRMTHWSGE
                     VEGKRIDLIIAGRGMEVTDAGSSVAIQDELNVLLELSPERVERVEFITPGREFAESKF
                     MKTHAGIEIPLPVASLLGVKVTSR"
     gene            complement(5063..6103)
                     /locus_tag="VF531_04915"
     CDS             complement(5063..6103)
                     /locus_tag="VF531_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7713342.1"
                     /translation="MVLGTNRCSAERKTVGEMIAVLGIDGGGTKTVALLAASDGKVLG
                     LGIGGPLNEKFIDERQARQSVVNSLEAAMSGVKSPIKIEAVYASAPGLTPEVLYSALK
                     EKQVSAKVFLEDDVLASFRSALLTDRGVVVLAGTGSFAVARNGHGDQAITGGWGPLLG
                     DEGSGYALGLEVLRSVLRSGEGRERPTCLTELLRNELKYRNKKELIDMVYDENMSRRK
                     IAALAMLVSQAVRMDDEVAIGIMSRAGVELASLGAAAAKNLGFAREDFLFTLTGGMAK
                     AGEPLLTSFRTEMCSLMPGGKYVPPKAEPVFGTLLLAFEKAGLKTNDELISDLHTQYT
                     EWRARTALTDEV"
     gene            complement(6094..>6393)
                     /locus_tag="VF531_04920"
     CDS             complement(6094..>6393)
                     /locus_tag="VF531_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713343.1"
                     /translation="NLASRHSSGKKLFEYAAVVLDNCVEYGDAIAGVDGLKQKMGPAS
                     TIVGAVILNSVMIETTKLLISWGMKVPVLMSSNIPGGSEYNAALIEEYKSRIRWF"
BASE COUNT         1689 a         1683 c         1298 g         1723 t
ORIGIN      
        1 cttttctttc ttcattggct tcattggccg caaatccaat taaatgtcca ttgatcgagt
       61 cttccaaaga ggtagttgaa atggagggag gcttaccgca taccacattg acaaaatccg
      121 ccacaagatg caaatcccct ccgccatgca tatcattgac gacattcaca tttacctttg
      181 cttcggaata ttcatggcct tttcgcgcat ccggtcgccg aatgataaaa tatccttctt
      241 ccataactcc ttcgatttca cccgttgtgc caatgatatg gatatagcga cagggctttg
      301 cagaattacc gttcatacta tgggttgcga tactgccatc ttcaaattca atctctactg
      361 tttgatgatc gacgacttcg ttgtcacagc gccaaacaca tcgtccatac ggattatctt
      421 tttgaagcga ttgaattttt tgttccagtg aaggtcccaa actcacggat tcaaccgaat
      481 cccaaacgta tgagccccat aaatcctgtt caatatagtt tttatatgcc gaaaagggac
      541 aggtggattc gattttacaa tctatcaaac acctggtccc cgcaccggca ggggccttgt
      601 ccggtgtaaa atacataaga ctgccaagct gctgacagcc cgaggtctga tattactttt
      661 catccaggtg attaagtcta gatcatgaca tgatttagcc attaacatgg ttgatttgca
      721 tacccttgga ttattccact ttccacggac ataggagact gccatgtgat ggtagctgac
      781 attttcttgc gtgttgatgc ttaggatttc cccgatctcc ccggcggaga ttcgtttgcg
      841 tattccggca taaaacgggg catgccgcaa tacatgacat atcatcacag tacgatttgt
      901 attgcgggcg gcagctaata gttttaaaac ttcttcccga tttaccccaa tgggtttttc
      961 cagtaaaaca tcataacctg cagacaacag gggtaatgtg gtttcactta tcttcccttt
     1021 cactttgacc ccatcggatt agttatccat gaattccgtg gtttttcagc agccttgcca
     1081 gcgtataccc taaaaaaccg gcacatgtgg caaaagctcc tcctgtcttt acaatgccgc
     1141 tctcgctgtc aaagctttca caagcaaaac cattatccag ttcaagctcc tcaatcattc
     1201 ggagccgcga ttcgtcatga aacaccaata aataattgca gatgctgata agccagggaa
     1261 acggggtatg aatacacccc gtgctgtcaa accgcggcga ctgatatcga tgcttattgt
     1321 gttccgaata aatccatttc aaagtgttcc ggtaaacttc atcatcccgg ctgcaaaaac
     1381 cgtaataggc aaggagtacc agactgcccg gcggattatc gtagatttca tgatggcccc
     1441 cgcaatctgt agaccaggca tacattggtc caaatactcc ctcagcaata ccatgttttt
     1501 cgatggcgat ccttaactcg tccgctgctt tcggagagac atatccgttt tggctcaaaa
     1561 acactaacgc cttccaaact agtacgttat catagatcag atacggataa gaaattggat
     1621 catccgaagg atctagctcg gtccggtata aacagtcttc cccggaatgt cgcctctcaa
     1681 gctcgcttac gaggtgatgt aaaccgcgtt gaatctcttc cgcatcaaga atatttttat
     1741 catgagtcca ttcgatatac tcttccagag ctatcacata tgccgcgagt tcatccaact
     1801 caaagccggg atataataca ctgccgtttg tgtatagact atggataccg gcattcttta
     1861 gatatcttgt aaagcaggtt tgcaatacct tttttgcttc ctggtgatcg atgatacaga
     1921 tggcgggaaa actccagaga agtgaatctc tggcccaaaa cgcggcactt acataatatt
     1981 tgggacttct tgatgttaaa agtacttgtt cttcagtatc aagagcatat ccagtagcaa
     2041 aaaaatatga aaacaacaaa ttccgattca tcttttcttc cagcgtcatc cggttttcca
     2101 aggaaggatc atccggctca acccgatgtt ctccgagcca tcgaagttcg tccgcataaa
     2161 gactctcacc gccgcgcctt ctcagatcga tattcacggc gccggctcca tccggctcca
     2221 tattgaccga gtagtaataa tagaacacct tttcctcatt gctcttaagc cttgttctat
     2281 tggtaactgc gtaagtcatt gccttttgat cataatccag ttcatagtcg ctatctccgg
     2341 agatccccaa ggcaactaaa gtaaggctgt cttttaattc cagtatcaaa gaatgggtcc
     2401 atcgatgcca agatatacac tttttcgcaa atatctcccg ggtatgaaat acggtagacg
     2461 aactttcagc ccagatcccc tcgactccgc atgtgatatc cagctccgca ttggttatat
     2521 ttttgacggt aattccatac acaagtcctt taaacccttt gggtgatata atccggacca
     2581 ttatccaaag cccggaaccc tcatattcaa atgaaggtat ccagcttttt tcataccagg
     2641 attttttggc cgtcaatgtt aatgatcgat tatgaatgcg gatttgcggc tttacgagat
     2701 acttcccgga gaaatcgatc aaactacggg ttcgatcaag aattacattc atcgtctcga
     2761 tctcgccgtt tcccaatatt gacggaatcg atagatactc gtttcctgtc gccagatatt
     2821 tccgatattc tttttcggta tcagggacag ttaatatctt ttccatggtt catacccgcc
     2881 ttggatcatt cattaccgga agttcaattg attaccggct ggttacttta actccgagga
     2941 ggctggctac gggaagagga atctcaatac ctgcatgggt tttcataaat ttactctcgg
     3001 caaattccct gccgggggtg ataaattcca ccctttccac ccgctcgggg gagagctcca
     3061 acaatacgtt taattcatct tggatcgcaa cggatgagcc tgcatcggtt acctccattc
     3121 ctctgccggc aatgatcaag tcaatccgct tcccctccac ctctcccgac cagtgggtca
     3181 tccgaatccg cggatcgtat cggatctcca agtcgtcgcg gaatgtacaa tgagcgaagt
     3241 aataggcgca tgcctgacgc atttttataa cattccggag ataacttcgc aaacaatcat
     3301 cctcggctac aaaacgttcc cccaattcaa cccataaaat gcttcccagg agcatcgccc
     3361 gctccacgtc acggtagaac cactggtgcc gaagtctccg ttccgggatt cggcggggat
     3421 ttaccatatt aatctggata taatggggga acgtgtattt atacaaattg aagaattcgt
     3481 cataaaatgt gccgttccag gtgagattgg caaaaatgta ctctccataa atatcaccgc
     3541 agttttcaat cataagaaaa gggggtgaat ccgggtcggc ggcctcaagt atcccgcgaa
     3601 tcatctccag ataaccctga ttaaataaac cgtgatgttc atggttatgg gcctgatcat
     3661 agcagggata cggtgtagca gagcccaatt gatcaagata gattcccttg gctccatacc
     3721 acttcactaa caccgttgca taatccacca ctcgttgttg ccatgcctta tccttgggac
     3781 acattaccgc aaagctcggg gggccgtatg tttctttgat ccacttccct tgttcgtctt
     3841 tgagcgccca ttccgttccc agggtaggga aatagtcgga atgaatgtca aagatccgga
     3901 cattgacgta aaaagtaacc attcccccgt gagcgttgat atactctacg cctctggcca
     3961 aatccagcgg gcttcccagt tccatatccg gaaaatactc gggataattg gagtcaaatc
     4021 cttgcctatt ccagccggcg atcaaaaaat gattgattcc gtcggccctt gcctcatcat
     4081 acatagcggg gatctcttta aatttgtgaa aaataccttc gttccgccga aaatcatacc
     4141 gcggacaaag caccgactgc gtcagtaaat cctcggcaaa ttccggatag acccgccctt
     4201 tattaaacca ggcccggtaa cgatcggcac cccagtgcca atcgcctcta tgaatgccaa
     4261 cggaataagg ttttgaagtc caagccgttc ccggctcgat cggcaaatat ttacggaacg
     4321 aaaaaccgac ccacggatca gcagggccgc cgacttccac cctgactccg gtcaggagat
     4381 agtcatcatc ataggaggcc aaatacaagc cgccgtccga atcatcataa taatccatcc
     4441 acatcatgga ggccaaaccg caataattaa tctcccgcga ataatacgat ccttctcttt
     4501 tactgccggc ccgatcgaac tgcagataac gctgggaagc atagttttct gcgggatttt
     4561 gaattttttc cccggcatgg tgcggataaa ccagaaaatc atcctggtgg gtatctccta
     4621 tccagatgcc gctgatataa ggaaagagaa cctcgacaat tttcaaattt tcggtgtgat
     4681 tctttatcga aagccgccag atgctttccg catcatccgg aaagcagaaa accgtcattt
     4741 cggcttgaac cgcccattcc tggccttctt catttaccat ggattgaaac ttgagtgaaa
     4801 tgacccgccc gtcttcagtc gattcgcagc ctgataaaac tcttggaaca agacgccgtt
     4861 tcctgcaatg atgatccaaa taaaataagg caaacggcat attcaatccg gctgcttttt
     4921 tctttacata attatccccg gtggttttat tgagcaggcc ggtaataccg ccatgagtgg
     4981 aagaaaaagt aatctcaaag agattattgc cggttccaaa atcggaatcc atgatatcac
     5041 ccgtcttcaa atgattttgt cgttaaactt catccgttaa cgccgtccgt gcccgccatt
     5101 ccgtatattg agtgtgaaga tcgcttatca attcatcgtt tgtttttagg cctgcttttt
     5161 caaaagccaa caaaagggta ccgaataccg gctcggcctt cggcggaaca tacttcccac
     5221 ccggcatcag gctgcacatc tccgttcgga atgatgtgag caaaggctca cccgccttcg
     5281 ccatgcctcc ggttaaggta aataaaaaat cttcccgggc aaaacccagg tttttggctg
     5341 ctgcggctcc aagggaagcc aactccactc cggcacggga catgattccg atggccacct
     5401 cgtcatccat cctgaccgct tgactcacca gcatagccaa agcagcgatc ttccggcgtg
     5461 acatgttctc gtcatatacc atatcgatga gttctttttt attccgatac tttaattcat
     5521 tccgaagtaa ctccgtcaag catgtaggac gttccctgcc ttcgccggat cgaagaaccg
     5581 aacgcaatac ttccaatccc agagcgtagc cgcttccttc atcgcccaac aaaggccccc
     5641 atcctccggt aatcgcctga tctccatgac cattccgggc tacagcgaaa gaacccgttc
     5701 cggcgagaac caccactccg cgatccgtga ggagtgcgct tcgaaatgaa gccaggacat
     5761 catcctctaa aaaaactttg gcggatactt gcttctcctt caaagccgaa tataaaactt
     5821 caggcgtaag tccgggagcg ctggcgtaaa cagcctcaat tttaatgggc gattttactc
     5881 cggacattgc cgcttcaagt gaatttacaa cagactggcg ggcctggcgt tcatcaatga
     5941 acttctcatt gagaggcccg ccgattccta atcccaatac tttaccatcg cttgcggcaa
     6001 gcaatgcaac ggtttttgta ccgccgccgt ctatcccaag tactgcaatc atttctccca
     6061 cggttttcct ttctgcggaa caccggttcg ttcctagaac caacggatcc tgcttttgta
     6121 ctcttcgatc aatgcggcgt tatattccga gcctccggga atattggaac tcataagaac
     6181 aggaactttc attccccatg aaatgagtaa tttagtggtt tcaatcataa cgctatttaa
     6241 aataactgca ccaacgatag ttgaagccgg tcccatcttt tgcttaagac catcaactcc
     6301 ggcaatggca tccccatact caacgcagtt atccaatacg acagcggcat actcaaaaag
     6361 cttcttgccg gagctatgac gggatgcaag att
//
LOCUS       DASVMB010000163         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_684308,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000163 DASVMB010000000
VERSION     DASVMB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684308"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VF531_04925"
     CDS             complement(<1..464)
                     /locus_tag="VF531_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX7713344.1"
                     /translation="MSHQAVLFDLDGTLLDTLADLGDSMNRVLLRAGFPEHPLEAYRY
                     FVGDGMEKLAFRALPADSRDSANTARITTAMKEEYDRHWADKTQPYPGIPELLAELDK
                     EGNPLAVFSNKPDEFTRKMIAAFFPGRRFGAVIGARPGVPKKPDPGVPLEIAA"
     gene            complement(504..1214)
                     /locus_tag="VF531_04930"
     CDS             complement(504..1214)
                     /locus_tag="VF531_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX7713345.1"
                     /translation="MSTNSRGFNGVTPEAVRFLAALRDHNNKPWFEDHKEQYQQDLLH
                     PFQALVSELAGVMLTIDPQFITVPSVNKTISRIYRDTRFSKDKSLFRSSIWLTFKRPS
                     IDWKEAPAYFFEITPERYRYGMGFYNASKVFMDRFRRAIREETAEFRGVTAFYREKQP
                     FTLEGEVYKRLLAPDLPEDLQEWYQRKNFYLTVNRTPDSLLYSKALTGELLEGFSTLA
                     PFYQFLWELKHRLEVERG"
     gene            1399..>2336
                     /gene="uvsE"
                     /locus_tag="VF531_04935"
     CDS             1399..>2336
                     /gene="uvsE"
                     /locus_tag="VF531_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX7713346.1"
                     /translation="MLVRLGYVANALRLEDSSPSRTVTLKSIHQLNDRQVQLNRLTRV
                     ARENLVNTLRILKANVYDGIPLYRFTSKLVPLCTHPEFADWDYAAPLQEDFRAIGEFI
                     NRHGLRVSLHPDHFTLLNSPSEEVWGASLRDLNYHQNVLLLMGLDETAKLIMHVGGKY
                     DSRQAAVARFTARYATLPPSIKNRLALENDDRCFTATEVLQLAEANRIPMVLDLHHHR
                     LLNHGEKVGELLPRVFQTWEADRPKLHISSSKSPGEPRNHADFIVPSEAAGFLNLSRE
                     LGRDYDLMIEAKQKDLALLKLAEELRHMGFNLPTPG"
BASE COUNT          570 a          628 c          585 g          553 t
ORIGIN      
        1 gcggcaatct ccagcggaac cccgggatcg ggttttttgg gaaccccggg ccgggctccg
       61 ataactgcgc cgaaccttct cccggggaag aaagccgcaa tcatcttccg ggtgaactca
      121 tcaggcttat ttgagaaaac agccagcgga ttcccctcct tatccaactc ggcaagcaat
      181 tcaggtatgc cgggataagg ctgcgtctta tccgcccaat ggcggtcgta ttcttctttc
      241 atggctgtag tgatccgggc cgtatttgcc gagtcgcggc tatccgccgg taatgcccgg
      301 aaggccagct tttccatacc gtctcccacg aaataccggt aagcctccag cggatgttcg
      361 gggaagccgg cgcgcagcag aacacgattc atcgaatcgc ccaaatcagc cagggtatct
      421 aagagggttc cgtcaagatc aaaaagtacc gcctgatgac tcatgctgcc tcctaaattc
      481 tgatggtaaa ctcaaggccc gaatcagccc cgttctactt ccaagcggtg tttcaactcc
      541 cagaggaatt ggtaaaaagg tgcgagtgta ctgaatcctt ccaatagctc cccggttaat
      601 gcctttgaat agagcaacga gtcgggggta cggttcacgg ttaaataaaa gttctttcgc
      661 tgataccatt cttgaagatc ctccggcaga tcaggggcga gcaacctttt gtagacctcc
      721 ccctccagtg tgaagggttg tttctcccgg taaaaggcgg taacccccct gaactctgcc
      781 gtttcctcgc ggatcgcccg tcggaaccgg tccatgaaaa ccttggacgc gttgtagaac
      841 cccattccat atcggtaccg ctcgggagtg atctcaaaaa aatatgcggg agcttctttc
      901 caatcaatgc tgggacgctt gaaagtcaac caaatcgaac tgcgaaagag cgatttatcc
      961 ttggaaaacc gggtatcccg gtaaatccgg gagattgtct tattaacgga cggaacggta
     1021 atgaactgcg gatcaatggt cagcatcacc ccggcgagtt cggagacgag agcctggaat
     1081 ggatggagta aatcctgttg atactgttcc ttgtgatctt caaaccaagg tttattatta
     1141 tggtcccgga gagcggctaa aaaccgaacc gcctcggggg taacgccgtt aaagccacgg
     1201 gaattggtgc tcatataatc cctccaaagg agccttgaaa gatatacctt caataagaat
     1261 ggtaaatccg ggttacttta gatcaggaat ataggacttt gtcgttcgcc aagaggtgca
     1321 atgcttgtcg ccttttgcgt gcctcgtgcc tcacgcctct tgccttattc aatacccccg
     1381 gaaaggatca atcgccctat gctggttcgg ttgggatatg tagccaatgc cttgcgcctg
     1441 gaggattctt ccccttccag gacagttacg cttaagagca ttcatcagct gaatgatcgc
     1501 caggtacaac tcaaccgttt aacgcgggtt gcccgggaaa acctggtgaa tactttgcga
     1561 atcctcaagg ccaatgtata tgacgggatt ccattatacc gtttcacttc taagctcgtc
     1621 ccgctctgca cccatccgga attcgcggac tgggattatg ccgccccgct acaggaagat
     1681 ttccgcgcca tcggcgaatt tataaaccgt cacggactac gggtcagcct tcaccccgat
     1741 catttcaccc tgcttaatag tccaagtgaa gaggtgtggg gcgcctcact acgggatctg
     1801 aattatcacc aaaacgtgct gttattgatg ggtttggatg aaaccgccaa gctgattatg
     1861 catgtggggg gtaaatatga ttctcggcaa gctgcggttg cacgttttac agcccgctat
     1921 gccaccctgc cgccatccat caaaaaccgt ttggccttgg aaaatgatga tcgctgtttt
     1981 actgcaaccg aagttttgca attggcggag gccaaccgga taccaatggt tttggacctc
     2041 catcatcaca ggcttctaaa ccatggtgaa aaagtcggtg agctcctgcc aagggtcttc
     2101 cagacctggg aggccgaccg gcccaaactc cacatctcca gttccaaaag tcccggtgaa
     2161 ccaaggaatc atgccgattt tatcgttcct tccgaggccg ccggcttttt aaacctcagc
     2221 cgcgaactgg gaagggacta tgatctgatg atcgaagcga aacaaaagga tctggccctc
     2281 ctcaaactgg cggaagagtt acgacacatg gggttcaatc tgccaacacc gggcat
//
LOCUS       DASVMB010000164         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_97341,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000164 DASVMB010000000
VERSION     DASVMB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97341"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_binding    complement(124..350)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(480..617)
                     /locus_tag="VF531_04940"
     CDS             complement(480..617)
                     /locus_tag="VF531_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713347.1"
                     /translation="MIIMPGTSMYPSSGYCDGLDGAACKQAEETSGFKAAPPPSLAVG
                     G"
     gene            977..1498
                     /locus_tag="VF531_04945"
     CDS             977..1498
                     /locus_tag="VF531_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7713348.1"
                     /translation="MTKDLIDAIEERRSIRQFETTPIPDATIGRLIESAHLAPSAGNL
                     EPWKFIVVKRDDLKAGLAEAARGQNFIVTAPVCIVVCADPQRSSARYGERGANLYCIQ
                     DSAAATQNILLTATGYGLGSCWVGSFDEEQVREVLGLDLNLRPMAIIPIGYPAAEPGT
                     IAKRSVDEVTLIM"
     gene            complement(1850..3412)
                     /locus_tag="VF531_04950"
     CDS             complement(1850..3412)
                     /locus_tag="VF531_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX7713349.1"
                     /translation="MKKYPVKPVVSDHDIEAVSHLPGRVIRLDFNGIKGKFNPMFKEC
                     IGAGRANEGLRADWQQQLVFVKKECDFKYIRMHGLLSDDMGVYKEDSQGNPEYNFQYI
                     DVLYDYILSIGMKPFVELGFMPAALASGSQTIFWWKGNITPPKDYKKWEALIRNLVLH
                     FTERYGEDEVAKWYFEVWNEPNLDGFWSGTQEDYFKLYKYSVQAIKSVNPEYRVGGPA
                     TAGAAWVPEMINFCSENALPLDFISTHAYGVKQGYLDEFGTTGTILDKNIWSVSGDIL
                     NSRQQITNSPMPNLELHYTEWSSSYTPADPIHDSYHQAAYILEKIKQVGNEANSMSYW
                     VFTDIFEEAGPRFTPFHGGFGLLNYQAINKPAFYAYSFLNKLGETELENTDSSSWACK
                     DSNSNLQILFWDFTNTHPGDSVNNQAYYIRDLPANPKGKIKVQISGIPEGNYLLEIYQ
                     IGYQVNDPYSTYLAMGKSKQLTRQQVDTIKKLNDGSPFLKEMIEINALEGFSKEIDIR
                     ENDVFLFNLIKI"
     gene            complement(3393..4613)
                     /locus_tag="VF531_04955"
     CDS             complement(3393..4613)
                     /locus_tag="VF531_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713350.1"
                     /translation="MRKWWSGFNREYKLLILVWLFFGLTSGLYDATFNNYLNEVFHLS
                     AKVRGYLEFPRELPGFLVAIISGLLIFLADTHLLGAALGLVTVGLVGQSFAIWHGQPQ
                     FNWMIFSMLIWSVGTHLYLPLSSSVSMRLSNPAEVGRSLGRLNGVNTAAYIAGCVLLL
                     LVTAFFKSQYRIIFWIAAFFCALAACCIFMIKVKPRVEKSDTKFRFVFRREYSLFYWL
                     NILFGARKQVFLTFAPWVLIKVYHQNEGTFAMLLIIASLAGIFFKPWLGKMIDRVGER
                     RILMGEAVSLVLVCLGYGFAGRLGLGTNAIYLVFTCYILDQLLFAVSMARSTYLHKNL
                     VTESDLTPTLSMGVSMDHMVSMTIPVLGGWLWEAYGFEAIFVVAALVACINFFVASRI
                     KEKVGGDLNEEISC"
BASE COUNT         1440 a         1297 c         1110 g         1326 t
ORIGIN      
        1 tcacccttgg cgtttacgga agatacatgt tctgaggact cgacgctcca aaggatgcga
       61 agacaagggg tgaatcaatt tcaggcactc atcctaactg aaagtataaa aaaaccgtcc
      121 tcaaaaaggg acggatttag ccgtggtacc acccagattg gtccaaatga agaacgaaca
      181 tttgaacccg ctcgataccg gtaacggcgg ctgccgtccg ggtttactgg aaacaattcg
      241 acccggaagc tcggggagga tcttcacgcc ggccttcgtt gcccctttgc accaaccggg
      301 gctctctcga aactccgtac ctgcgttact cgtcccgtca tcgctgttga cgaaacatac
      361 acatcaattt gttgttattg taaaccattc cactatagaa gtcaagagcc ccatgatcaa
      421 agtcgggtta tgatagctca tttagcttaa cctgctatct gaaattaaac aaaaattcat
      481 cagccaccca ctgccaggga gggcggcggc gcggctttga aaccggatgt ttcttcggct
      541 tgtttacaag ccgcgccgtc gagcccatcg caatacccag aagacgggta catggaagta
      601 cccggcatta taatcaaaaa ctcgtactcc gaacagggag gtgaggagat atcagggggt
      661 cttgggggct cagaagcata ggcgactttg acaaagcttg tttcccctct cctttgtttg
      721 ttaaggagag gggagaaaag gggtgaggtc gccgggggga tcctcgcgat gtaaatatcg
      781 cgtgggtgtc cggtcacacc ccttggccct aaggggtgcg gggaagtggg cgacgttccc
      841 cgtcgaccca agatattgct atacagacta tattgacccc aaaaaactaa ttcccagtct
      901 attaagttag atttatacaa ctttccgttg gcttcagggt aaactatcct cacatttata
      961 ggaggagttc atcgccatga ccaaagatct cattgacgcc attgaagaac gccgcagcat
     1021 ccgtcagttt gaaaccaccc cgatccccga cgccaccatc ggccgtttga tcgaatcggc
     1081 acacctggcc ccaagcgccg gcaatctcga accctggaag tttattgttg tcaaacggga
     1141 cgaccttaag gcgggccttg ccgaagcggc ccggggccaa aattttatcg tcaccgcccc
     1201 ggtatgcatc gtggtttgcg ccgatcccca gcgttcctcg gcacgttacg gcgagcgcgg
     1261 cgccaacctc tactgcatcc aggacagcgc tgcggcaacc cagaatatcc tactgaccgc
     1321 caccggttat ggactgggca gttgctgggt gggttccttc gatgaggaac aagtcaggga
     1381 agtattaggg ctggatttaa atttacgtcc catggccatc atccccatcg gctatccggc
     1441 tgcggaaccg gggaccatcg ccaaacgctc cgtggatgag gtgacgttga ttatgtaaaa
     1501 ttttggagga taatgataaa ttttagagaa ttaataaaat aaatttttga gcatgttcta
     1561 tctgatgata actttttagc ttattcatga ggccaaccac ccctatccct gcgcgcttag
     1621 tgaagcgcga cttcaggagg ggaaatttgt aatacaatgc ggggacaccc cgcaccccgg
     1681 cagcattgcc atgctgcact tgaccttggt aattggttaa ctggttttcc aaaagctaaa
     1741 ggatagaatg taacattaat gcagggacac cttgtgtcca atggacacga ctccccggca
     1801 gcacacttgc ctttgttttt atcgggatca accgtttctg gaaacatacc tatattttaa
     1861 taaggttaaa caggaaaaca tcattctcgc ggatgtcaat ttctttcgaa aatccttcga
     1921 gcgcgtttat ttcaatcatt tcttttaaaa acggagaacc gtcgttaagt ttcttgatcg
     1981 tatcaacctg ttgtcttgta agttgcttcg acttacccat tgcaagataa gtggaatacg
     2041 gatcattcac ttgataaccg atttggtaaa tttccaacag ataattgcct tccgggattc
     2101 cggatatttg tactttgatc tttccttttg gattggccgg taaatcacgg atgtaatacg
     2161 cttgattatt gaccgaatct cccggatggg tattggtaaa atcccaaaat agtatctgca
     2221 gattactgtt tgaatccttg catgcccatg aggagctgtc ggtattctcc aattctgttt
     2281 cacccagttt gttcaaaaaa gaataggcat agaaagcagg cttgttaatg gcttgataat
     2341 tcagtaagcc aaaaccgcca tgaaaaggag tgaatctcgg tcccgcctct tcgaaaatat
     2401 cggtaaacac ccaatatgac atggaatttg cctcattacc gacttgttta attttttcaa
     2461 gtatataggc agcttgatga taactatcat gaattgggtc agctggggta taggaagaac
     2521 tccattcagt gtagtgaagt tccaaattgg gcatgggaga gttcgttatt tgttggcgtg
     2581 aatttagaat atccccgcta acgctccaga tatttttatc taaaattgta ccggttgttc
     2641 caaattcgtc taaataacct tgttttacac catatgcatg ggtactgatg aaatcaagcg
     2701 gcagcgcatt ttcactgcaa aaattaatca tctcaggcac ccaggcggct ccggcggtcg
     2761 cgggtccgcc aacccggtat tccggattta cacttttgat ggcttgcaca ctatatttat
     2821 ataatttgaa atagtcttcc tgagtccccg accagaaacc atccaagtta ggttcattcc
     2881 aaacttcaaa ataccatttt gccacttcat cctcaccata gcgttcggtg aagtgaagga
     2941 ctagattgcg gattaaagct tcccatttct tgtagtcttt cggaggggtg atatttccct
     3001 tccaccagaa aatcgtttgg ctgccactgg caagcgctgc cggcataaag cccaactcca
     3061 caaacggttt cattccaatg ctcagaatat aatcatataa aacatcaata tattgaaaat
     3121 tgtattcagg atttccttga ctatcttcct tgtaaacacc catatcatcg gataacagcc
     3181 catgcatccg gatatattta aaatcgcatt ctttctttac aaagaccaat tgttgctgcc
     3241 aatccgcccg tagtccttca ttggcccgac cggcgccaat gcactcttta aacattggat
     3301 tgaacttacc tttgattcca ttaaaatcaa gccgaataac ccgtcccggt aaatgagaaa
     3361 cagcttctat atcatgatca gataccacag gtttaacagg atatttcttc atttaaatct
     3421 ccccccacct tttccttgat ccggctcgcc acaaaaaagt taatacacgc caccaaggct
     3481 gccactacaa agatggcctc aaatccatag gcttcccata gccaaccgcc caataccggt
     3541 atggtcatgg ataccatgtg atccatggaa acccccatcg ataatgtagg cgttaaatcg
     3601 ctctcggtca ctagattctt gtgtaaatag gtgctcctgg ccatggagac cgcaaatagc
     3661 agttgatcga ggatgtaaca tgtaaaaacc aagtagatcg cgttagttcc cagcccgagg
     3721 cgtccggcaa agccataacc caaacagact aaaaccaggc taacggcctc acccatcaaa
     3781 atccggcgct cccctacccg gtcaatcatc ttccccagcc agggcttgaa aaagatacct
     3841 gcaagggagg cgatgattaa gagcattgca aatgtccctt cgttctgatg atacacttta
     3901 atcaaaaccc agggtgcaaa ggttaagaag acttgtttgc gggcgccgaa caatatgttc
     3961 aaccaataga acaaagaata ttctctccga aaaacaaaac gaaatttggt atcactcttc
     4021 tctacccggg gcttaacctt tatcataaat atacaacacg cggccaaagc acagaagaat
     4081 gcggctatcc aaaagatgat ccggtattgg gatttaaaaa acgccgttac aagtaaaagc
     4141 aaaacacacc cggcgatata ggcggctgta ttgacgccgt ttaatctgcc caaactcctg
     4201 ccaacttccg ccggattgga taacctcatc gatacgctgg aagagagcgg caggtaaaga
     4261 tgggtcccca cactccagat aagcatacta aagatcatcc agttaaactg gggctgccca
     4321 tgccagatag caaatgactg cccgacaagc cccacggtaa ctaaaccaag cgctgctccc
     4381 agcaaatggg tgtccgccaa aaatatcagt agcccggaga tgatcgccac caaaaatccg
     4441 ggtaattcac gggggaactc caggtaaccc cggactttag ccgataagtg aaaaacttca
     4501 ttgaggtaat tattgaaggt tgcatcatat aaaccgctgg tcaagccaaa aaacaaccag
     4561 accaggatca gtagcttata ctcccggtta aaacccgacc accatttgcg catctggatc
     4621 cccctgaaaa agtagtatca atcgaaccat tatttaataa atatacaccg attcaaaagc
     4681 gttggcaatt catccaaaaa attcttgcga tgtatgccaa gagtaggtat atctcttagt
     4741 cgccataggg cctattcacc taacccccgg ccccttccct cattcagtga agccgacatc
     4801 taggacaagg aaggggtgac ttaatcgata aacttgatac tgatatgaat gatgctgtaa
     4861 tttgttacat cgttttccca ttcttttttt ctttatcttt tgaaatctgc cactaaccgc
     4921 ttgttcccat tttggctcaa tctttcaatc aaaagcaccc cttccctgtc ccagatactt
     4981 gcttctttga ccgaggaaag gggccggggg ttaggtcggc tgaatcttca cgacctcgca
     5041 ttgatgtgga atgacatcca tggcataggt cccgttaaaa acgcccacat ctttgcaggc
     5101 ccaaagatcc cggaccgttg ccttgatatc agtcgctaac cccagatcat gccagtcgac
     5161 gatgatagtt ttc
//
LOCUS       DASVMB010000165         5066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_21936,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000165 DASVMB010000000
VERSION     DASVMB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21936"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VF531_04960"
     CDS             <1..1053
                     /locus_tag="VF531_04960"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713351.1"
                     /translation="PFQVIIIEIIGGVTERNEEERLLTRIGSLNIIRQLLAEVDYVTV
                     FMDTSQRLVIINNNEEIDLNTLAERLKTSLINRLRHSFCIGVGNWHQGESGIRSSYRE
                     ACEALNYKVLIGKNEIIFYQDLHFYVSEEPYLSNEQVDMLGFYLKAGMGAQVGELLEL
                     IFKELAANQTISVDRIRVAGSNVIMKILNILTVLGIDTAGIFGKEGQPYEHVFVIDTL
                     PEMKEYIFGISMKTSNLINQIYHKKANKLVQEIQDYLIKNYHDSELSLTKVAKIFYVN
                     PSYLSRIFKQETNQTFVEYLTQIRMEKARKIFKETDLKAYQVAERVGITDPHYFGLCF
                     KKYTGISVNDFKKSQL"
     gene            1335..2456
                     /locus_tag="VF531_04965"
                     /pseudo
     CDS             1335..2456
                     /locus_tag="VF531_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            2615..3499
                     /locus_tag="VF531_04970"
     CDS             2615..3499
                     /locus_tag="VF531_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713352.1"
                     /translation="MDKVLADKKAIFVFIFPAFLIFTVIVIAPIFLSSYYSLLKWDGF
                     GKGLMVGLQNYRDLFINTSDGFLRSIVNSFILAGLSVFIQLPLALFFALILARGIRGE
                     NFLRTVYFIPVILSTVVIGQLWMKIYHPSFGLLNTFLTSIGLKSWAHPWLGSVDTALM
                     SAFIPSIWQYIGYHLLLMYAAAKSVPDELYEAAKIDGASEMATALRVTIPLIMPMIKV
                     CVVFAVIGSLKSFDLVYILTGGGPVHASEVPSTLMFNTIFFKYMYGYGSAMAVVIILE
                     CLVLTQVIQKAFKVEEIS"
     gene            3519..4403
                     /locus_tag="VF531_04975"
     CDS             3519..4403
                     /locus_tag="VF531_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713353.1"
                     /translation="MKTELNGSTLEIQRFPVKKRFFSPGKTIIIILLGLFAFIQLYPL
                     IWLALFSFKDNLEIMGGNIVGLPHRLLWQNYSQAFVGGKVGWYFVNSLIVTVVTIVVS
                     GVFSSMAAYAITRMKWGLSQTVLNIFLLGLMIPIHAALLPLFIILRDLKLLNSYWALI
                     IPYVAFALPMAIFIFTGFLQTIPRELEESACLDGCNIYQIFYHIIIPLIRPAIATVSI
                     FTYLSAWNELMFAVTFISKQEFKTLTVGIISLAGQYTTEWGPIGAGLVIATLPTVVAY
                     VLMSSQVQKSLTAGALKG"
BASE COUNT         1418 a         1042 c         1224 g         1382 t
ORIGIN      
        1 cctttccaag tgataattat tgaaattatt ggcggagtca cggaacgaaa tgaagaagaa
       61 cgcctgttga cccggatcgg ttccctgaac attattcgac agctgctcgc ggaggtggac
      121 tatgtaacgg tttttatgga tacgagtcaa cgtttggtaa tcatcaataa caatgaagag
      181 attgatctca atacccttgc cgaaaggctc aaaacaagcc tgatcaatcg attgcgccat
      241 tctttctgta ttggtgtggg gaattggcat cagggagaaa gcggcatccg ttcctcctac
      301 cgggaggcat gtgaggcatt aaattacaag gttttaatcg ggaaaaacga gatcattttt
      361 tatcaggacc ttcattttta tgtttccgaa gaaccgtatc tctccaatga acaagtcgat
      421 atgcttggtt tctatttaaa agccggaatg ggggctcagg tcggcgaatt attggagctc
      481 atttttaaag aacttgcggc caatcagacg atttcagtcg accgaatccg ggttgccgga
      541 tcgaatgtca tcatgaagat cttgaatatc ctaaccgtgc tggggatcga tacggccgga
      601 atttttggaa aagaggggca gccctacgaa catgtttttg tcattgacac gctgccggag
      661 atgaaagagt atattttcgg gatctcgatg aaaacatcca atctgatcaa tcaaatctat
      721 cataaaaaag caaataaact agtccaggaa attcaggatt atttaatcaa aaactatcat
      781 gacagcgaac tgtcgctgac gaaggtggca aaaattttct acgtcaaccc cagttactta
      841 agccggattt ttaaacagga aacgaaccag actttcgtgg aatatctcac gcagattcgc
      901 atggaaaagg ctaggaagat ttttaaagag accgatttaa aggcttatca agtcgccgaa
      961 agggtgggga ttactgatcc gcactacttt ggcctctgtt ttaaaaaata taccgggatc
     1021 tcggtgaatg actttaaaaa aagtcagctg taatcaaccg gaaagtaaaa ttttttacca
     1081 tttcagtaaa acggttgaat tgaggtgaat cgggttttcg attacccttt tctcaggtgc
     1141 ctgcgcgcgt gcgcgcgagc aaccctaaaa tttaaggagg gagattaatg cagaaaaggg
     1201 cgtttaaatc ggcacttctt ctcactttag ttgcagcctt gacgttcagt ttggctgcgg
     1261 gggccaagac gaaaaccgtc accttaaaac tgtggcatct atgggcggct gacactgagt
     1321 ccaacaaaat cccgatggaa aaagtcttaa gggattttgg aaaagcctac ccgaacatca
     1381 agattgaagt tgacgcggtg gagaatcaaa cttataaaac caagattaag acggcgaatg
     1441 ccgcgaatga agcccccgat atcttcttta gctggggcgc cggctttgcc caaccctttg
     1501 tggagtgagg aaaggtcctg gcgcttgatc cgtatctcaa agacggcacc aggaacaaga
     1561 tgctcggtgg aaccctagcc aatttcactt ataataaaaa ggtttacgga ttaccgattt
     1621 atatgtgggc cggagtcttc tattgcaata aagagttgtt cgaaaagaat aatattaaaa
     1681 ttccggagac atttagtgaa ctcatatcag cggtaaaggc tttccgggcc aaagggatca
     1741 ccccgattac cgtcggtgag aaagaacgat ggccgggcat gtttttccaa aatatcctgg
     1801 cgctccgtac cggcggaacc caattatgca tggatgctct aagtaagaaa gcctcgtttg
     1861 accagcctgc tttcattgaa tcagccgcga aactaagcga gctggtaaag gcgaatgctt
     1921 ttaacgaagg gtgtctgggg ttaacccggg atgagtccga ggctgttctg attcaaggtc
     1981 aggtgccgat gtactacaac gggagttggg tcgcaggttc cttggaaaag gatggttctc
     2041 cggtgcaggg gaagattgtg gccaaaaagt tccccatggt tgaaggcggc aaaggaaata
     2101 gcgatgaatt cttaggcggc tccatcgata cctttatggt tagcgccaat accaaatatc
     2161 ccaaagaagc cgcactggca gtcaagtata tctgtgagaa tatgtccagg gaagcttatc
     2221 tggcaggcgg aaatctgccg tcctggaagg tgaatgtcga caagtccaag attaatccgt
     2281 taatgcttca aattgtagac ttaacaaaga atgccaaggg ttttgtcctg gcttgggaca
     2341 ccttcctggt cggcgccgag gcggatgttc acctgaacct gattcaggaa attttcgccg
     2401 gaatgacgac cccggaaaag tttgccaaag aaatgcagaa attaaatcaa aaataaaccc
     2461 cacacggagt caataatcaa agtggttaag gaggcatctt cttaaccact ttgaatttgg
     2521 atatgaacga acgcttccaa ggtgggttta ctaataagat tttgatattc atttgatatt
     2581 caatcgtaat ccttggatat ttggggggcg gattatggat aaagttctgg ccgataaaaa
     2641 agcgatcttc gtgttcattt ttccggcatt ccttatcttt accgtgattg tcattgcgcc
     2701 catttttctt tcaagctact acagtttgct aaaatgggac ggatttggta aaggtttgat
     2761 ggttgggctg caaaactaca gggatttatt tatcaacact tccgatgggt ttctgcgatc
     2821 gattgtcaac tcctttatac tagcgggtct atccgtcttt atccaacttc cgctggcgct
     2881 tttttttgcg ctcattctgg ccagagggat tcggggagag aattttctcc gaaccgttta
     2941 ttttattccg gtgattctct ccaccgtggt catcggacaa ttgtggatga agatttacca
     3001 tccgtcattt ggacttctca acaccttttt aacctcaatc gggttgaagt cgtgggctca
     3061 tccctggttg ggcagtgtag atacggcctt gatgtccgcg ttcattccaa gtatctggca
     3121 gtatatcgga tatcatctct tactgatgta cgcggccgca aaatcggttc cggatgagct
     3181 gtacgaagcg gcaaagatcg atggcgcctc cgaaatggca accgctttaa gggtcacgat
     3241 tccgttaatc atgccaatga tcaaggtatg cgtggtcttt gcggtaatcg gctcattaaa
     3301 atcctttgat cttgtctata tcctgaccgg cggcggaccc gttcacgcaa gtgaagtgcc
     3361 aagtacatta atgttcaata caattttctt taaatatatg tacggttatg gcagcgccat
     3421 ggcggtagtc atcattttgg aatgcttggt tctaacccag gtgattcaaa aggcgtttaa
     3481 ggttgaggaa atctcttaag aaaaggaggg aaacaccgat gaaaacggag ctgaacggtt
     3541 caacacttga gatacagcgg ttccccgtta aaaagcgttt tttcagtcct ggcaaaacca
     3601 ttatcatcat cctgttagga ttatttgcgt ttattcaatt atatcccctg atatggctgg
     3661 cccttttttc atttaaggat aatttggaaa tcatgggggg aaatatcgtt ggactgccgc
     3721 accggctgct ctggcagaat tatagtcagg cgtttgtcgg cggcaaagtt ggctggtatt
     3781 ttgtaaacag tctgattgta actgtagtga ccattgtagt ttccggtgta ttcagctcca
     3841 tggctgccta tgccattacc agaatgaaat gggggctcag tcaaacggtc ctcaatatat
     3901 ttctattggg attgatgatc cccatacacg cggcgttgtt accgctattt attattttaa
     3961 gagatttaaa actgttaaat tcgtattggg ccttgatcat tccttacgtc gcctttgcat
     4021 tgccgatggc aatctttatt tttaccggct ttttgcaaac aataccgcgc gaacttgagg
     4081 agtccgcctg tttggacgga tgcaatatct atcaaatctt ttatcatatt attatccccc
     4141 tgattcggcc ggcgattgcc accgtttcga tttttacata tttgtcggcc tggaatgaat
     4201 taatgttcgc cgtgaccttt atcagcaaac aggaatttaa gacactcact gtcggtatta
     4261 tctcactggc cgggcagtat acaacggaat ggggaccgat cggagcgggt ttggtcatcg
     4321 ccacactgcc cacagttgtg gcttatgtct taatgagcag ccaagtgcag aagagtttga
     4381 ccgccggggc tttgaaagga taaaatttct aagaggcaag aggcaagagg caagaggcaa
     4441 gaggcaagag gcaagaggca agaggcaaga ggcaagaggc aagaggcaag aggcaagagg
     4501 cacgataaac gcggttgttc agcactcact cttgaagaat atgatcaaat ctcttacccc
     4561 acggcatggc tcctttttca aaagatccgt tccgccatgg cggctaaaga atgggattat
     4621 tttttaagcg gtattgtagt aatcgatgtc gctcactatg gcggagtgat tgaaaacaag
     4681 aaacggggcc tagcacagaa caagccagag ttttagtatc cgaaatggta gaggaaggaa
     4741 gcgtcatgcg ctccgacagt tacaaatcgc cacgctcaaa gattacgtat gtgacgccat
     4801 aaagggccgc cgcctcttga atcagtgatc attcaagagg ttaatttttg atgcgggttc
     4861 atcaggccgc ccggattcga tgcaacaaaa agataaaaac acgctggttc atctcacagt
     4921 cagttacatt ttagtccaag gcaataactc gaaattcttc ggttttggaa ggtaaataat
     4981 agagattgtg gaaaatagga ataaatatga tagaagatta aatgcaatct aatacttttt
     5041 cataaaccta acgaaaggat gattcc
//
LOCUS       DASVMB010000166         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_389375,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000166 DASVMB010000000
VERSION     DASVMB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389375"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF531_04980"
     CDS             <1..736
                     /locus_tag="VF531_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7713354.1"
                     /translation="LLVLTPTRRANREQIKLAGMEQYDVNGDCFRWLNNVRQQYQDYA
                     QNIFIGGLGGCRGDAYKPGESLSREEAYRFHQYQIRALVEAGVDFLIAETLPALEEAL
                     GIAGVMSQTGCPYMISFVIRSDGTVLDGTRISDAIRAIDETVSPAPLCFMVNCVHPSV
                     LARAIAFNEDRELICRRVWGIQGNTSRKSPEELDNALELQTEEAIPFAQSMMELYQAY
                     GLKILGGCCGTNERHIEELARRLMVS"
     gene            1262..3049
                     /locus_tag="VF531_04985"
     CDS             1262..3049
                     /locus_tag="VF531_04985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7713355.1"
                     /translation="MGKEILPKTDSRPHQGFHDLAIGTKVTLYYLGLLTLSIAISGFL
                     YYRINMNIMSRKVSDISLQTLGSISANIYSVIDNVNNYSKGVLSDSTVQECLRNSEKR
                     TNLQVQRNINRYLIGLIDMTPLISAVYLFDNFGNKYGADKLTMKSFRLGDIKEAEWYE
                     DASKKKGGYILRLNAGNVFYKSNTGEPYISLIRLVNDLNTQKPIGVLIINTPCSFLGK
                     SFLGVENRSYTGIIVKDEKNQPMVRVNDVFGVNIDRIIPADQKNYYSLTRKIHGKWYL
                     LSALKMNRYGWRIISVIPFNELSRESGIFSMIAFAIIILNSLLIFLGSVFISKLITVP
                     INKLLKSMKNVEKGKFEIVNIQTGNDEIGKLKDGYNIMIQQIQELLERTVAEQKIIRK
                     TELEVLQAQIKPHFLYNTFDAISSLALAGRSNDVYTVMKSLGSYYRISLSKGNQIITI
                     REEIETVKNYLTIQQIRYGDIFSAIFQVDEELADLPVLKLVLQPLVENALYHGVKPKG
                     EKGVITIRVRREQENMELIVEDDGVGMDQSKLAEIMNPLHEKDRSSFGLQGTIERLRI
                     FYGREDIFKIESAAGEGTVITISIPMKEF"
     gene            3053..>3491
                     /locus_tag="VF531_04990"
     CDS             3053..>3491
                     /locus_tag="VF531_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713356.1"
                     /translation="MNSQLLKILIVDDEALIRDLLKRCVDWNNLGLEVVGEASSAREA
                     MEIMEKQLPDIVFTDICMPHMDGIELSKSIGERYPQTKVVIITGYEDFDYAQRSIKAG
                     VADYILKPINDEEISRIAVKVKEMIEAERSKTTEYERLKRQLEE"
BASE COUNT         1044 a          689 c          874 g          884 t
ORIGIN      
        1 gttattggtt ttgacaccga cccgccgggc caaccgggaa caaatcaaat tggcgggtat
       61 ggaacaatac gatgtcaacg gcgattgttt ccggtggctg aataacgtcc ggcagcaata
      121 ccaagattac gcacaaaata tattcatcgg cgggctcggc ggatgcaggg gtgatgcgta
      181 caaacccggt gaatcactga gccgcgaaga agcatatcgc tttcatcaat atcagatcag
      241 ggctttggtg gaggctgggg tcgatttttt gatcgccgaa accctgcctg ccttggaaga
      301 ggctttggga atcgccgggg tgatgtccca aaccggttgc ccgtatatga ttagctttgt
      361 aattcgttcc gacgggacgg tactggatgg gacccggatc agtgatgcca tccgggccat
      421 cgacgagaca gtttccccgg cgcctttgtg cttcatggtg aattgcgtac atcccagtgt
      481 actggcacgg gctattgcat tcaatgagga tcgggagttg atttgcagga gggtatgggg
      541 gatccaggga aacacttccc gcaaaagccc ggaggaactg gataacgctc tggaactcca
      601 gaccgaggag gctattcctt ttgcccagtc catgatggaa ctgtatcagg cgtatggcct
      661 caaaatcctc ggcggatgct gcggaaccaa tgagcggcat attgaggaac tggcgcgacg
      721 attgatggta agttaagctc ttatcgcgat aacccttatg catccataag ggttattttt
      781 ttcccttatg ggggaggcac attatcacca tcatcccgac catcatcaag gggcagatta
      841 gtgatatcag ccagctaagg ttggaggaaa taccaacctt ggatacaagc aaaacaagcc
      901 ccaggggtac caggcagcat aagagcatca tcagcaaatg cgcggtgtgg ccttttccgg
      961 aatggctttg gcggcatgac gggacttctt tttcctcggg ttcctgaccc atactatttc
     1021 acctcgttga gtaatgatga aatctttgtg atgagtacat gatatatcaa agaagtgaag
     1081 gttctatgaa gatcgtaaaa aaagtagcag gtgaattcgg catgatgtgg tatatgaatg
     1141 gtaaatgatg gatcaaattc aaatacctac ccaacatgtt accaagaggt atatactacc
     1201 ggataaattg cagatgacac tggttggacg gaatatcaat aaaaacaaag tggagagtga
     1261 aatgggaaaa gaaatcctac ctaaaacaga ctctcggccc caccaggggt ttcacgattt
     1321 ggcgattgga accaaagtca ctttgtacta cctggggtta ttaaccttat cgattgccat
     1381 tagcggtttt ttatattaca gaattaacat gaatatcatg tcccggaaag taagtgatat
     1441 ttcattgcaa accttaggtt cgatcagcgc caatatatac agtgtcatcg ataatgtcaa
     1501 taattactcg aaaggggtgc tgtcggattc caccgtacag gaatgtctca gaaactccga
     1561 aaaaaggact aacttacaag tccaacgcaa tattaaccgg tatttaatcg gattaatcga
     1621 tatgaccccc ttaatatcgg ccgtatatct gtttgacaac tttgggaata aatacggggc
     1681 tgacaaattg acgatgaaat ctttccggct gggagatatc aaggaagccg aatggtatga
     1741 ggacgcttcg aagaaaaaag gtggttacat tctccgttta aatgccggaa atgtttttta
     1801 taaatcgaat accggtgaac cttatatttc actgatcaga cttgtcaacg atctgaatac
     1861 ccaaaaaccg attggggttt tgattatcaa tacaccctgc agtttcttgg ggaaatcttt
     1921 tctgggtgtt gaaaacagaa gttataccgg cattatcgtt aaagatgaaa agaatcaacc
     1981 catggtcaga gtaaatgacg tattcggggt taatatcgac cggattattc cggccgatca
     2041 aaaaaactat tattcattga cccgtaaaat tcacggaaag tggtacttat tgtccgcgct
     2101 aaaaatgaac aggtacggct ggagaatcat cagtgtaata cctttcaacg aactctcccg
     2161 ggagtcaggt attttcagca tgattgcctt tgcgatcatc attttaaaca gtctattgat
     2221 cttcctcggc tcggtcttta tctccaagtt gattaccgtc ccgatcaata aattattaaa
     2281 atccatgaaa aatgtagaga agggcaagtt tgaaatcgtg aatattcaaa ccgggaatga
     2341 cgaaattgga aagttaaagg atggttacaa cattatgatt cagcaaatcc aggagttact
     2401 ggagcggacc gtagcggaac aaaagatcat ccgaaaaaca gaattggaag tactgcaggc
     2461 ccagattaaa ccgcattttc tttataatac ctttgatgcc atcagttcgc tggcactggc
     2521 cggccggagc aatgatgtat atacggtcat gaaatcactg ggcagttatt accggattag
     2581 cctaagtaaa ggaaatcaga tcatcaccat ccgtgaagag atagaaacgg tcaaaaatta
     2641 cttgaccatc caacagattc gttatggaga tattttctcg gcaatctttc aggttgatga
     2701 ggagttggcc gatttaccgg tgcttaagtt ggtgttgcag cctttggttg aaaacgccct
     2761 ttaccatggg gtaaaaccga agggggaaaa gggcgtaatt acgataaggg ttagaagaga
     2821 gcaggagaat atggagttaa tcgtggagga tgacggggtg ggcatggatc aatcgaaact
     2881 tgccgagatc atgaatccgc ttcatgagaa agaccgttcc agttttgggt tgcaagggac
     2941 tatcgagcgt ttacgaatat tttacggcag agaagatatt tttaaaatag agagcgccgc
     3001 gggggaaggt accgtgatta cgatttccat tccgatgaag gagttctgaa ggatgaatag
     3061 tcagctactc aagatattaa ttgttgacga tgaggctcta atcagggatt tgctgaaaag
     3121 atgcgttgat tggaataatc ttgggctgga agttgtcgga gaggcctcgt cagcccggga
     3181 agcaatggaa atcatggaga aacaattgcc ggacatcgtg ttcaccgata tttgcatgcc
     3241 gcatatggat gggattgaac tcagcaaaag tatcggagaa cggtacccgc aaacgaaagt
     3301 cgtaattatc accggatatg aggattttga ctatgcccag agaagcatca aagccggtgt
     3361 cgccgactac attctaaaac cgattaatga tgaagagatt tcccggatcg ctgtcaaggt
     3421 caaagagatg atcgaggccg aaagaagcaa aaccactgaa tatgaaaggt taaagagaca
     3481 gcttgaagag a
//
LOCUS       DASVMB010000167         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_685764,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000167 DASVMB010000000
VERSION     DASVMB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685764"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1300
                     /locus_tag="VF531_04995"
     CDS             143..1300
                     /locus_tag="VF531_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7713357.1"
                     /translation="MDEPIYIDIGWTGLSKEGEELCGDNVEIVRTPEAVVAVLADGLG
                     SGVKANILSKMTAKIIATMFEKGAQLEEIVETVSNTLPVCQKRRIAYSTFTIVQVNRD
                     GETYIVEFDNPGVIYLHKGQIFGTRTKEIEIYGKKMKESRFRLVPGDILTVVSDGVVH
                     AGIGGVLNLGWQWENVAEYLQREAAVEPSAVSLSRCLSNACERLYAGKPGDDATVLVL
                     GVRNLKTLTVAVGPPENRGDDPLLVRMIQEERGSKVVCGGTTSTIVARGLGQRIEVEL
                     EQMDPVIPPRGRIPGIDLVTEGIITLSKTLEYLKTVHSPEEIPRADNGATSLAKALFE
                     SDRIRFLVGRAINPAHQNPNLPINLGLKIQAVNELAEILRRSGKQVEIHYF"
     gene            1376..1903
                     /gene="nuoE"
                     /locus_tag="VF531_05000"
     CDS             1376..1903
                     /gene="nuoE"
                     /locus_tag="VF531_05000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7713358.1"
                     /translation="METAALPKKPEAAIGPRRKFEKVNEIIARHGKQQAALIPILQEV
                     QEEYRYLPEEILTYIATVMGLSPATVFGVATFYAQFSLEPKGKYVIKVCDGTACHVRG
                     SNPVRETIQKRLALKDNQKTTADLRYTLETVSCLGACGLAPVMMINDRIYGQMTAAAV
                     EIIINQLETEAGETQ"
     gene            1900..3810
                     /locus_tag="VF531_05005"
     CDS             1900..3810
                     /locus_tag="VF531_05005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7713359.1"
                     /translation="MIETRTKLNELKDAWARAYQKEPARNLVCGGTGCVANGSLKIFE
                     AIQAEVARRGALIQVEMMLENGENIPEKGTTVVKSGCHGFCELGPLVRLEPSGVLYTK
                     VKCEDAAEIVTALLDGKEPVERLLYHHPVTGALYAKEMQIPFYQHQTRNVLEHCGAID
                     PEEIRETIAEGGYQALAKVLLEMTPEAVCKEIIQSGLRGRGGGGFPTGRKWEATRVAP
                     GSQKYVICNGDEGDPGAFMDRSVMEGDPHKVVEGMAIAGYAVGASEGYIYVRAEYPLA
                     VKRLQIAIRQAREYGLLGPNILGAGFGFDIHIKEGAGAFVCGEETALMASIEGKRGMP
                     RPKPPFPAQSGLWGKPTLINNVETYANIGKIITNGASWFNSQGTPQSPGTKTFALTGQ
                     IANTGLIEVPMGITLREILFEIGGGLRQGKKFKAVQIGGPSGGCLTEEHLDLKLDFDS
                     LLSVGAMVGSGGLVVMGDDTCMVEVARFFMSFTQNESCGKCVPCREGTKRLLEILQRA
                     VDGEGRPEDLDILEDLAGAIKDGSLCGLGKTAPNPVLTMLKYFKDEYQAHVIEKRCPA
                     GVCKALSAYCIDPDLCKGCGKCAKNCPVQAISGTIKNPHIIDASKCIKCGACVGSCPF
                     KAIAIGGGTQNE"
     gene            3803..>4216
                     /locus_tag="VF531_05010"
     CDS             3803..>4216
                     /locus_tag="VF531_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7713360.1"
                     /translation="MSELKNSVIIDGQTVELNGETSLLELIRKCGIDIPTFCYNSELS
                     VYGACRMCVVEIEGRGIQASCSTPPAPGMKVLTNSPRVQRVRRTVLELLLANHNRDCT
                     TCEKSGHCKLQDLANRYGINRVRFGERDIKLPIDDS"
BASE COUNT         1019 a         1049 c         1286 g          862 t
ORIGIN      
        1 gaggtcatca acaagcaaat gagggtggct caggagattg ccggtttgct cggggagacc
       61 accgccgaaa ccaaagtact tttgaccaag ttgatgagtt tgctccagga taagccggat
      121 tcaccgagga aggagaggcc aaatggatga acccatatat atcgacatcg gatggacagg
      181 cctctccaag gagggtgagg agttatgcgg cgataatgtc gagatcgtcc gtactcccga
      241 ggctgtagtg gcagtgttgg ccgacgggtt gggcagcggg gtcaaggcga acatcctttc
      301 caagatgact gccaagatca tcgccaccat gtttgaaaaa ggagcccaac tggaagaaat
      361 tgtggaaacg gtcagcaata ccctgccggt ctgtcaaaaa cggcggatcg cctactccac
      421 ttttaccatc gttcaagtta acagggacgg tgagacgtat atcgtggagt tcgacaaccc
      481 gggtgtcatt taccttcata aaggtcagat cttcggaacc cggactaagg agatcgagat
      541 atacggcaag aagatgaaag aaagccgctt ccggctggta cccggcgata tactgactgt
      601 tgtctccgat ggagtggttc atgccggtat cgggggagtc ttgaatctcg gttggcagtg
      661 ggagaatgtc gccgaatact tgcagcgcga ggcggcggtt gaaccaagcg cggtcagcct
      721 ctcccgctgt ttatcaaatg cctgtgaacg gctttatgcc gggaaaccag gtgatgatgc
      781 aaccgttttg gtgctcgggg ttcgcaatct aaagaccttg accgtggcgg tggggccgcc
      841 cgagaatcgt ggcgatgacc ccttactcgt ccgaatgatc caggaggaac gtggtagtaa
      901 agtggtttgt ggcggaacaa ccagtaccat cgtggcccgg gggctcgggc agcgtatcga
      961 ggtggaactg gagcaaatgg atcccgttat tccaccccgg ggaaggattc ccgggattga
     1021 cctggtaacc gaggggatca tcacgctcag caaaacgctg gagtatctga aaaccgtcca
     1081 cagccccgag gagattccac gggctgacaa cggcgccact tccctggcca aggcgctttt
     1141 cgagagcgac cggatacgtt ttttggtcgg ccgggcgatc aatccggccc atcagaaccc
     1201 caacctcccc attaatttgg gattaaagat ccaagccgtg aatgagttgg ccgagatttt
     1261 aaggcgatcc gggaaacagg tggaaattca ctacttttaa cccggagaag gattggcaat
     1321 aacggcggaa ggccaagacc ggatgccgat ggaatcattg aaaggagtta aacagatgga
     1381 aacagcagca ttaccgaaga aaccggaagc cgctattgga ccgcgccgga aatttgaaaa
     1441 ggtgaatgag atcattgccc gtcatggcaa acaacaagca gcgcttattc ccatcctcca
     1501 agaggtacaa gaggagtacc gttacctgcc ggaggagatc ctgacttaca ttgccacggt
     1561 tatgggttta tctccggcca cggtttttgg agtggcgact ttttacgctc aattttccct
     1621 ggagcccaag ggaaaatatg tcatcaaagt ctgtgatgga accgcctgtc acgtgcgcgg
     1681 ctcaaatccg gtgcgggaaa cgatccaaaa gcggctggcc ctcaaggaca accagaagac
     1741 gacggcggat ctccggtaca cgctggagac ggtttcctgt ctgggcgcct gcggtctggc
     1801 accggtgatg atgattaacg accggattta tggccaaatg actgcggctg cggtggagat
     1861 tatcataaat caactggaga cggaagcggg tgagacccaa tgattgaaac acggactaaa
     1921 ctcaatgagt taaaggatgc ctgggcccgt gcctatcaaa aagaaccagc ccggaatctt
     1981 gtttgcggcg gcaccggttg cgtggcgaac ggttccttaa agatttttga agccattcag
     2041 gcggaagttg cgcgaagggg cgcgctgatc caagtggaga tgatgctcga gaacggtgaa
     2101 aacattccgg aaaagggcac cacggtagtt aaaagcggtt gccacggttt ctgtgaattg
     2161 ggccctttgg ttagattgga accgtcgggc gtgttgtaca ccaaggtgaa atgtgaggat
     2221 gcggcggaga tcgtaaccgc cttactggat gggaaggaac cggtggaacg gttgctttat
     2281 catcatcccg tcacaggcgc attatacgct aaagagatgc agatcccgtt ttaccagcat
     2341 caaaccagaa atgttctgga gcattgcggc gcaatcgacc cggaggagat ccgggagact
     2401 atcgccgaag gcggctacca ggcactggcc aaagtgcttt tggaaatgac gcccgaggcc
     2461 gtatgcaagg agatcattca gtccgggtta cggggccggg gcggcggggg gttccccacc
     2521 ggcaggaaat gggaggccac ccgggtagct cccggcagcc aaaaatatgt catttgcaac
     2581 ggggacgaag gtgaccccgg ggccttcatg gaccggagcg tgatggaggg cgacccccat
     2641 aaagtggtgg aaggaatggc cattgccggt tatgcggttg gggctagcga agggtatatc
     2701 tatgttcggg ccgaataccc tttggctgtg aaacgactac aaatcgccat tcgtcaggca
     2761 agggaatacg gcctgctggg tccgaatatt ctcggcgccg gtttcggctt tgacattcat
     2821 atcaaggaag gcgcgggcgc ctttgtctgc ggcgaggaga ctgcgctcat ggcttccatc
     2881 gaaggcaagc ggggcatgcc ccggcctaag ccgccgtttc cggcccaatc cggtttatgg
     2941 ggtaagccga ccctgatcaa caacgtcgag acatatgcca atatcgggaa gatcatcaca
     3001 aacggcgctt cctggttcaa cagtcaggga acaccccaga gccccggtac caaaaccttc
     3061 gccctgaccg gacagatcgc caataccggt ttgatcgagg tgccgatggg gatcaccctg
     3121 cgggagattc tcttcgagat cggcggcgga ttacgccagg ggaagaagtt caaggcggtc
     3181 cagattggtg ggccctccgg cggttgtctc accgaggagc atctcgatct caaactggac
     3241 ttcgattccc tgctttccgt gggggcgatg gtcggctccg gcggattggt cgtcatgggc
     3301 gacgatacct gtatggtcga ggtggcccgg ttctttatga gtttcaccca aaatgagtcc
     3361 tgcggcaaat gcgttccctg ccgggaaggg accaaaagac tgctcgagat attgcagcgg
     3421 gcggtggacg gagaaggccg tcccgaggat ctggatatcc tggaggattt ggccggagcc
     3481 atcaaggacg ggtctctctg cggattgggt aaaaccgcgc cgaacccggt gctgacgatg
     3541 ttgaagtatt tcaaagatga ataccaggcc cacgtcatcg agaagcgttg tcccgccggg
     3601 gtgtgcaagg cattaagcgc ctactgtatc gaccccgatc tctgcaaggg gtgcggcaaa
     3661 tgcgccaaaa actgtccggt gcaggccatc tccgggacga ttaaaaatcc gcatatcatt
     3721 gacgcttcca aatgcattaa atgcggagcg tgcgtcgggt catgcccatt caaagctatc
     3781 gccatcggag gtggaactca aaatgagtga actgaaaaac agcgtgatca ttgacggcca
     3841 aaccgtggag ttgaacgggg agacgagcct cctagagctc attcgcaagt gcgggattga
     3901 tattccaacc ttttgctata actcggagtt aagcgtctac ggcgcttgcc ggatgtgtgt
     3961 ggtggagatc gaaggccgcg gcattcaagc ctcctgttcg acgccgccgg cgccggggat
     4021 gaaggtcttg accaatagcc cccgggtgca gcgggtacgg cggaccgtcc tggaactgct
     4081 tttggcgaat cacaaccggg attgcactac ctgtgaaaag agcggtcatt gtaagttaca
     4141 ggatctggcc aatcgatacg gaatcaaccg ggtccgattc ggggaacggg atatcaagct
     4201 ccccatcgat gattcc
//
LOCUS       DASVMB010000168         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_537956,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000168 DASVMB010000000
VERSION     DASVMB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537956"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..>1589
                     /locus_tag="VF531_05015"
     CDS             337..>1589
                     /locus_tag="VF531_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase"
                     /protein_id="HEX7713361.1"
                     /translation="MGKIADQYLQTNPWYIIEDDFNPSRSRVSESIFSLGNEYMGVRG
                     YFEEGFNGQALIGSYFNGLYEETEIKHPALYKGFPTITRDLTNSVDWLYIRILLDDES
                     LDLSQSTYSNFHRKLDLRTGTLSREFVWQTVTGKRLKITFLRFVSMVSPHLGCQQVSF
                     EPLNFSGTIQVTTGLDFSPHADWFTASNWTCPRMEQNGDILAILGKTNRSNRYLFSSY
                     RLDCNDSTENCVVKAEKFIGFESKVHLKQGKTTLMTRIAVNYAGVGANTSGAEQNPRL
                     KPETVWSRGMELADGHRKTQFETALAEHSRYWENVWNQLDVTIEGDNENQQGIRFCIF
                     QLHQTYHGVDPALNIGAKGLTGEAYGGKAFWDTEAYCLQFYMFNNPRAARNLLEYRYQ
                     LLPKAVERARQLDCEGACYPISSIDG"
BASE COUNT          453 a          370 c          339 g          427 t
ORIGIN      
        1 aacctaattt taaagtcaac atctttttct cattttctac gaggggtgat tgaaggttaa
       61 atcatttatc aaaatcaata aagatgacct tttgaaacca atacggccgc ggcatgatat
      121 ttagatccac taaaagtcat cggaagaatc gaagtattct atatgtccga tttctgccaa
      181 ttttttcact ctgccaactg cccgccggca ttcgaaaaca tggaatttgc aagtgttatg
      241 gagttttttt atcacaaaaa gtgattgaca gtcaaattta acaggcgtta taattattgt
      301 taatcggtta acctttagtt caataaggag cacactatgg gaaaaatagc agaccagtac
      361 ttacaaacaa atccttggta catcattgaa gatgatttta atccatcccg cagcagggtt
      421 tccgagtcca ttttttcatt aggcaatgaa tatatgggcg tacgtggata cttcgaagaa
      481 ggttttaacg gtcaagcgct gatcggaagt tacttcaatg gactttatga agaaacagag
      541 atcaaacatc ccgcgctcta taaaggattt cctacgataa cccgggattt gaccaattcg
      601 gtcgattggt tatatatacg aattcttttg gatgatgaat cattagatct ctctcaaagt
      661 acctactcaa atttccaccg caaactcgat cttagaaccg gaaccctaag ccgcgaattt
      721 gtctggcaaa ctgtcaccgg caagagactc aagattacgt tcctccgttt tgtcagtatg
      781 gtctctcccc atctgggttg tcagcaagtt tcgtttgaac cgttgaactt ttccggaacc
      841 atccaagtta ccaccggatt agatttctct ccccacgccg attggtttac cgcaagcaat
      901 tggacctgtc cccgaatgga acaaaatgga gatattctgg caatcctcgg caaaaccaac
      961 cggagcaatc gctatctttt ctctagttat cggcttgatt gtaacgattc aacggaaaac
     1021 tgcgtcgtca aagccgaaaa attcatcggc tttgagtcca aagttcacct gaaacaaggt
     1081 aaaaccacgc tgatgacccg aatcgccgtc aattatgccg gtgtcggcgc caatacctca
     1141 ggtgccgaac aaaacccccg gcttaagccg gagactgttt ggagtcgcgg catggaactg
     1201 gcggatggtc accggaaaac ccagttcgaa actgccctgg cggagcattc ccggtattgg
     1261 gagaatgtct ggaaccaact ggatgtcacc attgaaggtg acaacgaaaa tcaacaggga
     1321 atccgctttt gtatctttca gcttcatcaa acctaccacg gagttgatcc ggctttaaat
     1381 atcggggcca aaggccttac cggagaagct tatggcggca aggcattttg ggacaccgaa
     1441 gcctattgcc tgcaatttta tatgtttaac aaccctcgcg cggccagaaa cttactggag
     1501 tatcgctatc agttacttcc caaagccgtg gagagagccc ggcagctcga ttgtgaaggc
     1561 gcctgttatc cgatctcatc aatcgatgg
//
LOCUS       DASVMB010000169         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_315981,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000169 DASVMB010000000
VERSION     DASVMB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315981"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VF531_05020"
     CDS             <1..641
                     /locus_tag="VF531_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713362.1"
                     /translation="FYPFLSGYRNLVHYANMHPQASRERLCEVIAMVGLEARIDDPVR
                     TYSLGMRQRLGIAQALLHYPSVLVLDEPTNGLDPGGIRELRRYLRHLAENEGVTAIVS
                     SHLLSEMELMCDRAAFIKEGKLLDIKAVRNFPRDEWQVFQVDQPQRARVEILKMFPGA
                     RVNIDEGAVELMAGRETTAAINAGLVQAGVKIYGIMPKTGRLEDVFMEVTSE"
     gene            638..1435
                     /locus_tag="VF531_05025"
     CDS             638..1435
                     /locus_tag="VF531_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713363.1"
                     /translation="MKELVNLIRSENMKIYFRKSTWVMVGFLILAVILSAATTWRIGV
                     NDGIWGLVAGQISALFLVDLFTVIVAAGIVATEYHWGTIKLLLIRPVNRGRILFAKYL
                     SLILFGLFLMMVLFGTALTVNGLLFTIKSGSGAELLGAVLPGKKLWFDSFGGATLLYT
                     LRFAEVAVYATFAFMLSTISKSSAFTIGISIFTMLFGPEFANMLSGKPWGKYILFANL
                     DLSRYLKNNPNPWPGMSPAFSTLVLIGYGLVFYLISRTFFIKRDVLS"
     gene            1653..2330
                     /locus_tag="VF531_05030"
     CDS             1653..2330
                     /locus_tag="VF531_05030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7713364.1"
                     /translation="MFLNNKLKALTFSYDDGVEQDRRLIKLFNQYNLKATFNLNSGML
                     SNANQWDNKGVCIKRMNVKGLKELYQGHEIAVHGYSHPHLEKLDIDTIRNEIEQDKIN
                     LQNMFDCTIRGMAYPFGTYDQRVIQVLKENNISYGRVVETTHNFDVQTNLLEFKASCH
                     HRDVNLMKLAEAFVELQPAEPKIFFIWGHSYEFDVDNNWEVIEAFCEFISNREDIFYG
                     TNSEVLL"
     gene            complement(2335..>2516)
                     /locus_tag="VF531_05035"
     CDS             complement(2335..>2516)
                     /locus_tag="VF531_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713365.1"
                     /translation="WLSPNSDSSHFDKLEMLWDAYFDSIERILGAPAGSLKVFGAEAF
                     QDWFREEQSDTAPVR"
BASE COUNT          690 a          513 c          653 g          660 t
ORIGIN      
        1 aattctatcc ttttttaagc ggataccgga atttggtcca ttacgccaat atgcatcccc
       61 aagcgagcag ggagcggctt tgcgaggtta tcgccatggt tgggctggag gcgcgaattg
      121 acgacccggt ccgtacttac tccttgggaa tgcggcagcg tttggggatc gctcaggctt
      181 tgcttcacta cccgtcggtt ttggttctgg atgagcccac caatgggctg gatccgggag
      241 ggatcaggga actgcgccgg tatttacggc acttggcgga aaatgaggga gtgacggcca
      301 ttgtctccag ccatctgctc tcggagatgg agttaatgtg cgacagggcg gcattcatca
      361 aagaagggaa gctgcttgat attaaggcgg tgcgcaattt tccccgggac gaatggcagg
      421 tttttcaggt agaccaaccg cagcgggcca gggtagagat tttaaaaatg tttcccggtg
      481 ccagggttaa tattgacgag ggagcggttg agcttatggc cgggcgggaa actaccgcgg
      541 cgattaacgc aggtttagtc caagcggggg ttaagatcta cgggatcatg cctaaaaccg
      601 gacgactgga ggatgtcttt atggaggtga cctcggaatg aaggaactcg ttaatctaat
      661 ccgcagtgag aatatgaaga tttacttcag aaaaagcacc tgggtgatgg ttggtttttt
      721 aatcctggca gtcattctgt cggcggcgac gacttggagg atcggagtta acgatgggat
      781 ttggggcctg gtggccggac agatatcggc cttgtttctg gtcgatttgt ttacggtcat
      841 tgtcgccgca gggatagtcg ccactgaata ccactggggg accatcaaat tattattgat
      901 ccgcccggtt aaccgcggcc gcatcctgtt tgccaaatac ctgtcgctga tattattcgg
      961 tctgtttctg atgatggttt tatttggaac agcgctgaca gtaaacgggt tattgtttac
     1021 aatcaaaagc ggcagtgggg cggaactttt aggcgccgtt cttccgggta aaaaactctg
     1081 gttcgactca ttcggcggag caacgctgct ttatacactg agatttgccg aggtggcggt
     1141 gtatgcaaca ttcgccttca tgctgtcgac catctccaaa agcagtgcgt tcaccatcgg
     1201 catttctatt tttaccatgt tgttcggacc ggagtttgcc aatatgttat ccggaaaacc
     1261 atggggaaag tatattctct ttgccaacct ggacctcagc cggtatctta aaaacaatcc
     1321 caatccgtgg ccggggatga gtccggcgtt ttcaacactg gtattaatcg ggtacggtct
     1381 ggtcttttac ctgatatccc ggaccttttt tatcaagcgg gatgtactat cgtgagtttt
     1441 gggccatatc gtgagaaccc aggcacccaa aggaggttat tcgagtaaac cggagtccgg
     1501 cgattatttt agaagagcca tgccgctatt taaggaaatc gttaaggaga aatgacaacc
     1561 caaatcagat tgtaatgaaa gagttattga aaaattgaat aaaaaatagt aatttaaatg
     1621 gcaaggagct tcaataaagg aggacaaaac ggatgtttct aaacaacaaa ttaaaagcgc
     1681 tgaccttcag ctatgatgat ggagtggaac aggatcgccg tctgataaag ctttttaatc
     1741 aatataatct aaaagccacg ttcaatttaa actcaggaat gttaagcaat gcaaatcaat
     1801 gggataataa gggcgtttgc ataaagcgaa tgaatgtgaa gggattaaag gaattatatc
     1861 aggggcatga aattgcggtt cacggatata gtcaccctca cttagaaaaa ttggatattg
     1921 atacaatcag aaatgagatt gaacaggaca aaatcaacct gcaaaatatg tttgattgta
     1981 cgattcgtgg aatggcatat cccttcggta cttacgatca acgggtcatt caagtcctta
     2041 aagaaaacaa catcagctac ggcagggtag tagaaaccac tcacaacttt gacgtacaaa
     2101 cgaatctcct ggaatttaaa gccagttgtc accatcgtga tgtcaatctt atgaaactag
     2161 ccgaagcgtt tgtagaattg caacccgcag aacctaagat cttttttatc tggggacata
     2221 gttatgagtt tgatgtcgac aacaattggg aagtcatcga agcgttttgt gagtttatca
     2281 gtaatcggga agatatattt tatgggacta acagtgaggt tttattatag aaccttatcg
     2341 gaccggagct gtatcggact gttcttctcg aaaccaatcc tgaaaagcct ccgctccgaa
     2401 aaccttgagg gaacccgcag gcgccccgag aatccgttcg atggaatcga aataagcgtc
     2461 ccaaagcatc tccagtttgt caaaatgaga tgaatcggaa ttcggtgata gccata
//
LOCUS       DASVMB010000170        10488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_22528,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000170 DASVMB010000000
VERSION     DASVMB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22528"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VF531_05040"
     CDS             <1..756
                     /locus_tag="VF531_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX7713366.1"
                     /translation="TGAIEATGHKVISIPVSDGKINPQVIQSVLEAHTDEHMVKPKLV
                     YISNPTEIGTIYRKSELEALSRFCKKNSLYLFVDGARLGSALCSPENDLKLSDLGVLT
                     DAFYIGGTKSGALLGEALVICNPSLQEDFRFHLKQKGAMLAKGRLLGMQFLELFRDDL
                     YFDLAKHANAMADLLRDALQKGGFRFLTHSSTNQIFPILPNSLIEGLQKKYSFIVWAK
                     MDPTHSAVRLVTSWATKEEAVLAFIEDLKTTGV"
     gene            complement(775..1101)
                     /locus_tag="VF531_05045"
                     /pseudo
     CDS             complement(775..1101)
                     /locus_tag="VF531_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
     gene            complement(1052..1798)
                     /locus_tag="VF531_05050"
     CDS             complement(1052..1798)
                     /locus_tag="VF531_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7713367.1"
                     /translation="MMELFEVNTKQGNTIEYYKKEADRNKIPVVLSMGNWEPALRGFP
                     LLNQIEDRTSVVLSYRGRGRSGSPDQGYDWKDHSHDLEAVVEDCGFDKCIFIAFSKGV
                     SYTLGYLESNPEKACGLLLIDYPAIHCESIKGYAEFWYNRTYKDFRLKDYVTRKALDG
                     IERESTYRDFYPLIASIKCPVTLFVGRDDQAPIRSNVEIEDVEKYQKANPNIRIIEFT
                     QSGHMILDDEPDKAYREVKKFLSGQQKPFC"
     gene            complement(1857..2675)
                     /locus_tag="VF531_05055"
     CDS             complement(1857..2675)
                     /locus_tag="VF531_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7713368.1"
                     /translation="MMIALDKDRCKKCGACIKMMKGYCITESNGFPIFDGTVCNTCQK
                     CVSICPSRAIMVNGNYPDKIDQTKEVNPEQIFSLFEKRRSIKHYQDKAISREIIEKIV
                     SVAKYAPNQNKNLSVLVIDDKHLINEIDQAAIAFVKTMYRILFGFKPITFLARLFYKD
                     IEALKRKMEHHSFQRVLNENTQAILIVTGNKKIPVTERSAYYMLGTIMYMAEALGIGN
                     CLMDSIYLALRTNRRLRKRLKIKEDVFGVLVLGYSNENVQNIPRGYEVNVRWNV"
     gene            complement(2691..2924)
                     /locus_tag="VF531_05060"
     CDS             complement(2691..2924)
                     /locus_tag="VF531_05060"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7713369.1"
                     /translation="MGSVFTSIDEYGLHGKRKERVFIGFYPGASAVEAFQNELSQHKH
                     AKGSVQFSISEPLPMELIQRIVKFRVEENRSKK"
     gene            complement(2900..3937)
                     /gene="add"
                     /locus_tag="VF531_05065"
     CDS             complement(2900..3937)
                     /gene="add"
                     /locus_tag="VF531_05065"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX7713370.1"
                     /translation="MTESKISDSISAALIERLRAMPKAEIHVQIEGATAPETFYQIAQ
                     HNQIELPAQSLEEWETFFEFRNFPHFIQVYTAAVGCLQTPEDYALMIESFFKRQAEQN
                     ILYTEAFLSASFLTRKFEDAAILDAIARGVEAGKRKYGVRVNLIPDISREIPNSQARV
                     LELVLKGKERGLFIGLGLGGMEAEYPPELFTETYTEARRQGLRVVAHAGEAAGAGSIW
                     GAINSLYAERIGHGIRCLEDPKLVEYLRQHQLPLEVSPQSNYRLGVVAATEPHPIRRM
                     VDSGLYCTVNSDDPAMFSTDLVNEYVTLASQGFSWEELWQLNLNTLKAAFLSQTEKAG
                     YLDQWDQFLRA"
     gene            complement(4085..4993)
                     /locus_tag="VF531_05070"
     CDS             complement(4085..4993)
                     /locus_tag="VF531_05070"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7713371.1"
                     /translation="MSEVEVYYFSGTGNSLAVAREITKKINGKLISIPSVIEQDSIRI
                     DADLIGIVFPVFYATNDCGVPLIISRFVGKLENLGSKYIFAVCTSGYMAGETIENLAK
                     MVRSRGGLLAAGFVINMSNKSLHDELQQKMHKSFGNVSVVEPVNRLKNGNINKEIYQR
                     KFQDKLELVAKTVNARNKAKLETRGILGKIVFMPLLLLIKPVFKLRYEKLSNASRLPF
                     RDLIPLADRSFQINEKCDGCGICARVCPVNNIKIVDGRPEWQHHCETCFGCYQWCPRE
                     AIHGEIVKYNERYHHPDVKVSDWYDQ"
     gene            5148..5909
                     /locus_tag="VF531_05075"
     CDS             5148..5909
                     /locus_tag="VF531_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7713372.1"
                     /translation="MNIDLTGKVAVITGSTGELGRVMARTLAQCGSDIAIHYLKNEAN
                     AQKLLNEIHLLGRRAVAVQADITDFDSVLKMRDTIVGTLGAPDIIVNNAVIQYQWKNL
                     LDQDIKDYESQFRSCVMQNVYMAKAFIPLMIPKKNGRFIGINTECAIQTFPNQSAYAA
                     GKKGMDAIYRVLAKEIGEHQITVNQVAPGWTVSEKDRLSGTEHQAAYEANVPLKRRGT
                     DQEIANVVAFLASDLASFITGAYIPVSGGNVIPGI"
     gene            complement(6009..6794)
                     /locus_tag="VF531_05080"
     CDS             complement(6009..6794)
                     /locus_tag="VF531_05080"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7713373.1"
                     /translation="MEKHHQESIAKFIGIYQNDPTILAILLGGSIAHGFAKPDSDIDV
                     TIIVDEAEYQKRKQENKLAFSLWDICNYPGGYVDCKVVSIDFLRMIIDRGSDPARYAY
                     KDSNILFSRIDDLQKILEDIGRFPIRVKNTRRIRFVSQLLAWKWYYSEAIKKQNQYLI
                     YLSLQKIILFACRIILNENNLLYPYHKWLLSVVRNARNKPEGFDESIEQVMAEHTPDF
                     VKQFCDDVLHFVGVDEKLLDWPNQFLLDSELNWVEHEAPVDDL"
     gene            6989..7519
                     /locus_tag="VF531_05085"
     CDS             6989..7519
                     /locus_tag="VF531_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7713374.1"
                     /translation="MVQPCPSGTFPYIILPRDNFYILAERYNTTVEAISAANPGIEPN
                     NLSVGQSICMPATKPPNPCPCGSRIYLVQAGDSLYSIAQKYNVPFDVLYTANIGLDPA
                     NLQIGQAVCIPQLPACPANSVAYAIEPGDSYYTLSKVTGVPIHDLIIANPGIDPNNLQ
                     AGQVICLPYRPTPTLY"
     gene            complement(7749..8612)
                     /locus_tag="VF531_05090"
     CDS             complement(7749..8612)
                     /locus_tag="VF531_05090"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7713375.1"
                     /translation="MMEIYYFSGTGNSLVVARDIAAKTKGRLISIPTIVNKQRIKTDA
                     DCIGLVFPSYLAQLYGIPLIVEKFVKQLDDIGTKYIFAICTCGGYRSVNALPPLKNLA
                     RLIRSLGGKLSAEFSIRLPMNNLDYAHIPVPINKDQEIMFKKCQKKIETICQCISERK
                     LNKYRALHSLINLLVTPICLLIRGTCIVSLKKYAKEPEDTKLKYYELIPLTDRSIYVD
                     DKCMGCTTCAKVCPVQNIIIVDKRPVWQHKCEMCFACAEWCPLKAIHHWGRAEGIYYH
                     HPRVKVTDMFR"
     gene            complement(8689..9144)
                     /locus_tag="VF531_05095"
     CDS             complement(8689..9144)
                     /locus_tag="VF531_05095"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7713376.1"
                     /translation="MKLKVLVGSGKKIGLFTLPFLVIGLILNIMFPSLFSVGGPSTAL
                     AVISIVILIPGVINWIWSVALILTKVPQKKLITDGPYSLVKHPLYAGVALLVFPWAGF
                     LFNTWLGVLIGIIVYIGSRIFAPEEEAILSKIFGATWDEYCNKVKIPWF"
     gene            complement(9309..10091)
                     /locus_tag="VF531_05100"
     CDS             complement(9309..10091)
                     /locus_tag="VF531_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713377.1"
                     /translation="MQKSIYEKSVAYLESDPLKHITTLKYLLLYRDQLTVDLTEDSKN
                     WALLVTIPTQILSFDTATYPYTNYAIFINGTADGLKYELLETLPNSNYILRLNESLDL
                     SKLRNRFDISKGKSYISYSCSSMDDSFKDGIVPGNTVITDEAIDIIKRNGYTESEIRW
                     HFNNGAVWFGLLKENRIRSICFVYQNYDDIWEIAGVHTLETERGKGYARIVVASALAY
                     LFEKKLTPRYMAEIRNTKSIQLAESLQMKQFLRMDHFLLRSL"
BASE COUNT         2796 a         2545 c         2083 g         3064 t
ORIGIN      
        1 acaggcgcca tcgaagctac cggacataaa gtcatctcca tcccggtgag cgacggcaag
       61 ataaatcccc aagtcatcca gagcgttctt gaagcgcata ccgacgagca tatggtcaag
      121 ccgaagctgg tctacatatc caatcccacc gagatcggca ccatatatcg aaaaagcgaa
      181 ctggaagcgc tctcccggtt ctgcaaaaaa aacagcctct atttatttgt cgatggcgcg
      241 cggcttggtt cggctttgtg ttccccggag aatgacctga aattatccga tcttggtgta
      301 cttacggatg ccttttatat cggaggcacc aaaagcgggg ctttgctcgg agaggcgctg
      361 gttatctgca atccttcgct tcaggaagat ttccggttcc atttgaaaca aaaaggggcg
      421 atgttggcca aaggccggct gttgggcatg caatttctgg agctctttcg ggacgatctc
      481 tatttcgact tggcgaaaca tgccaacgcc atggccgatt tgctccggga tgcgcttcaa
      541 aagggaggct tccggtttct cacccattcc tcaaccaatc aaattttccc gattttgccc
      601 aattcgctga tcgaggggct tcaaaagaaa tactccttca ttgtttgggc taaaatggac
      661 ccaactcact ccgccgttcg tctggtcacc tcttgggcca ccaaagaaga agcggtatta
      721 gcatttattg aagatctaaa aaccaccggg gtctagtgaa ccaatcaagt tgcttcatga
      781 ttcgcacgat gattttaaaa actcatcctt caacatggaa taatacaatc gatcaacata
      841 ttgtccatcc atgtagaaga aatcccgtaa tgtcccctca taagtaaatc cacatttttc
      901 aattacccgc tttgagggaa tgttaatgga cttatggcag atctgcacct tatgcaggtt
      961 tattttttcg aagccatatt gaatcactgc tttggtcgct tccgtcgcca gtccctttct
     1021 ttgaaaacgg cttccgatac aatactcgat ttcagcaaaa tggtttttgt tgtccactaa
     1081 gaaatttctt aacttcccga tacgccttat ccggttcatc atccagtatc atatgtccgc
     1141 tttgtgtaaa ttcaataatt cgaatattgg gattggcctt ttgatatttt tcaacgtcct
     1201 ctatttccac attacttcga atcggtgcct gatcatctct tcccacaaaa agcgtcaccg
     1261 gacatttaat acttgctatc agcggataaa aatcccgata tgtcgattcc ctttcaatcc
     1321 cgtcaagggc ttttcgggtt acataatctt ttaaccggaa atccttgtat gtccgattgt
     1381 accagaattc ggcgtatcct ttgatcgact cgcaatggat tgccgggtaa tcgatcagca
     1441 gtaacccaca tgccttttcc gggttacttt ccaagtatcc aagcgtataa gagacccctt
     1501 tggaaaatgc aatgaatatg cacttatcaa atccgcaatc ctcaactaca gcctcgagat
     1561 cgtgtgaatg atccttccaa tcgtagccct ggtccggaga accgctccgc ccccttcccc
     1621 gataactcag gactacactg gttcgatcct ctatttgatt aagcaatggg aaaccgcgca
     1681 aggcaggttc ccaattcccc atggacaaga caaccggtat cttgtttcga tctgcttcct
     1741 ttttgtaata ctcaatcgta ttcccctgtt ttgtgttaac ctcaaacaat tccatcattt
     1801 cctcccaaat ccttaatgca ccaaaacatc cttttttttg tatcgaaacg ctgcatttaa
     1861 acattccatc tcacatttac ttcgtatcct ctcggaatat tttgaacgtt ctcattggaa
     1921 taccccaata ccaacacacc gaatacatcc tctttgattt tcaatctctt tcgcaatctc
     1981 cgattggttc gtaatgccaa gtatatcgag tccatcaagc aattcccgat tcctaaagcc
     2041 tcggccatat acattatggt ccccaacata taataggcac ttcgttccgt taccgggatt
     2101 tttttattac cggtgacaat caagatcgct tgagtgttct cattgagcac ccgctgaaac
     2161 gaatgatgct ccatcttcct tttgagggct tcaatatctt tgtagaatag acgggccaaa
     2221 aaggtgattg gcttgaatcc gaataggatg cggtacatcg tctttacgaa agcgattgca
     2281 gcttgatcga tctcattaat gaggtgttta tcatcaatca ccagaaccga taaattcttg
     2341 ttttggttcg gcgcatactt ggccacagat acgatttttt caatgatttc tcttgagatt
     2401 gccttgtctt gataatgttt gatcgatctg cgtttctcaa atagagagaa tatttgttcc
     2461 ggattaactt ccttggtttg atcgatttta tccgggtagt ttccattgac catgatagct
     2521 cgtgaggggc agatcgagac gcacttttga caagtattac agaccgtacc atcaaatatt
     2581 ggaaagccgt tactttccgt tatacaataa cccttcatca ttttgatgca tgcgccgcat
     2641 tttttacatc tgtccttatc aagggcgatc atcattgaaa tccctcctgc ctacttcttg
     2701 ctacgatttt cttccaccct aaattttacg atgcgttgga tgagttccat cggcaacggc
     2761 tctgagatgg aaaattgaac cgaccctttg gcgtgtttat gttgagataa ctcattttga
     2821 aatgcctcga cggcgcttgc tcccgggtaa aagccaataa atactctttc ttttcttttt
     2881 ccatgaagcc cgtattcgtc tatgctcgta aaaactgatc ccattgatca agatacccgg
     2941 ctttttcagt ttgactcaaa aatgccgctt tcaaagtatt aagattcagt tgccacaatt
     3001 cctcccaaga gaaaccctga gaagccaggg tcacgtactc gttcaccagg tccgttgaga
     3061 acatggccgg gtcatccgaa ttgacggtac aatataatcc ggaatcaacc atccgtcgaa
     3121 tgggatgggg ttcggtagcg gcaacaacgc ccaggcggta attgctttgc ggtgagactt
     3181 ccaaaggaag ctgatgttgc cgcagatact cgacgagttt tggatcctcc aagcatcgaa
     3241 tcccatggcc aatccgctca gcgtataggc tgttgatcgc tccccatatg cttccggctc
     3301 cggccgcttc gcctgcatgg gcaacgaccc tcaatccctg ccgccgcgcc tcggtgtagg
     3361 tttcggtaaa tagctccggc ggatattcgg cttccattcc gcccaggccc aaaccgataa
     3421 ataaaccccg ctccttgcct tttaagacca attcaaggac ccgcgcttgg gaattgggga
     3481 tttcacggga aatatcagga atcaaattca cccgcacccc atacttcctt tttcctgctt
     3541 cgactccccg ggcaattgcg tccaggatgg ccgcatcctc aaatttccgg gtaagaaacg
     3601 aagcactgag aaacgcctcg gtgtaaagaa tgttctgttc ggcttggcgt ttaaaaaagc
     3661 tttcaatcat cagcgcataa tcttccggag tctgtaaaca tccgacagcg gccgtataca
     3721 cttgaataaa atgcgggaaa ttgcgaaatt cgaagaaagt ctcccattct tcaagcgatt
     3781 gggcaggtaa ctcaatctgg ttatgctgtg caatttgata aaacgtttcg ggagcggttg
     3841 ccccctcgat ttgcacatga atctcggctt tcggcattgc ccggagccgt tcaatcaacg
     3901 ccgcagaaat tgaatcagaa atcttggact cggtcatgaa gtcacgcgcc tcttttttct
     3961 tttagtctgc ctaatgttgt taccgcaccg ttaatggtat tttcatcctc attttcagtg
     4021 cccgacttaa cgatgagatt aagggatccg aagctggttt tccgatccac tctgcccgta
     4081 tatatcattg atcataccaa tcggacactt ttacatcagg atggtgatac ctctcattgt
     4141 acttcactat ttcaccgtgg atcgcctctc gcgggcacca ctgataacat ccaaaacagg
     4201 tctcgcaatg atgctgccat tcaggcctgc catcgacgat tttaatatta ttgacaggac
     4261 aaactctggc acagattccg cagccatcgc atttctcatt aatttgaaaa cttctatccg
     4321 ccaggggtat gagatcacga aagggcagcc gggatgcatt tgaaagtttt tcatatcgca
     4381 gtttgaatac gggcttaatc agtaaaagaa gcggcataaa tactattttg cccaatatac
     4441 cgcgcgtttc taatttggct ttgtttcggg cattcaccgt tttggcaacc aattcaagct
     4501 tatcttggaa cttcctttga tatatttctt tattgatatt cccgtttttc aaacgattaa
     4561 caggttctac aacagataca ttaccaaacg acttatgcat cttttgctgt aattcatcat
     4621 gcagtgattt attgctcata tttatcacga atcctgccgc cagtaacccg ccgcgggaac
     4681 gtaccatttt ggccaaattt tctatggttt ctcccgccat gtatccggat gtgcagaccg
     4741 caaatatgta ctttgaaccg aggttttcca atttcccgac aaatctgctg atgatcaacg
     4801 gaacgccgca gtcatttgtc gcataaaata ccgggaatac tatcccgatc aaatcagcgt
     4861 cgattctgat actatcttgc tctatcacag aaggaatgga tattagtttc ccatttatct
     4921 ttttcgtaat ttctcttgcc accgctaaag aattcccagt acccgaaaaa taataaactt
     4981 caacttcact cataatgaat cccctccatt ctaatagctt ttctatccta gggtcaatag
     5041 gccccaattc cagcctatcc accctttctg tttacaagca aagcaaaccg tattatgata
     5101 atagtaatca ttatggcagg atattccatg caatgatgga ggcgctcatg aatatcgatt
     5161 taacgggtaa ggttgctgta atcaccgggt ctaccggcga attgggaaga gtgatggcgc
     5221 ggacgcttgc ccagtgcggc tcggatatcg ccattcacta cttaaagaac gaagcaaatg
     5281 ctcaaaagtt gttgaacgag atccaccttc tgggccggcg ggctgtggcg gtccaggcgg
     5341 atattacaga tttcgattcc gttttaaaaa tgcgggatac gatcgtcgga accctcggcg
     5401 caccggatat cattgtcaac aatgccgtca ttcaatatca gtggaagaac ttattggacc
     5461 aggacatcaa ggattatgag agccagttcc gttcctgtgt tatgcaaaac gtctatatgg
     5521 ccaaagcgtt tattccgctg atgattccca agaaaaacgg caggtttatc ggcatcaata
     5581 ccgagtgcgc cattcagacg tttcccaacc agtcggccta cgccgctgga aaaaaaggaa
     5641 tggatgccat ctaccgcgtt ctggccaagg agattggcga gcatcagatc accgtcaacc
     5701 aagtggcgcc gggctggact gtcagcgaaa aggatcggct gagcggcacc gagcatcaag
     5761 ctgcatacga ggccaatgtc ccattgaaac ggcgaggcac cgatcaggag atcgccaatg
     5821 ttgtagcctt cttagcctca gatctggcaa gtttcattac cggagcttat atccccgtgt
     5881 ccggcggcaa tgtaatcccg ggtatataac gccctcaccc atcgggccga ttcaagtcag
     5941 tggccctctc ccattacgct cgaagagcac tcgcttatgt gagagggaga aatgggccta
     6001 agcacctctc atagatcatc aaccggagct tcatgttcta cccagttcaa ctcgctatct
     6061 agtagaaatt gattgggcca atccagtaat ttttcatcca ctcctacgaa atgcaacacg
     6121 tcatcacaaa actgtttcac aaagtccggg gtatgttccg ccattacctg ttctatagac
     6181 tcatcaaaac cttccggctt attccgggca tttcttacca cactcaacag ccatttatgg
     6241 taaggatata acagattatt ctcatttaat atgatgcgac aggcaaatag gattattttt
     6301 tgaagcgaca ggtatatcaa atactgattt tgctttttga ttgcctcact gtaataccat
     6361 ttccatgcta atagctgtga gacaaatcgt attcttcggg tatttttaac ccggatcgga
     6421 aaccgcccta tatcctccaa aatcttttgc aggtcatcga tcctggaaaa taagatgtta
     6481 ctgtctttat aggcataccg ggcaggatca ctgcctctat cgataatcat gcgaagaaaa
     6541 tcaatactga caaccttgca atctacgtaa ccgccaggat aattacaaat atcccatagg
     6601 ctgaaggcaa gtttattttc ttgcttccgc ttttgatatt cagcctcatc cacgatgatg
     6661 gttacatcga tatcggagtc gggtttggcg aaaccgtggg caatggaacc tcccaataag
     6721 attgccaaga tggtcgggtc gttttgataa ataccaatga attttgcgat ggattcctga
     6781 tgatgttttt ccattgtgcc actccctaat taagttgttt caccctggaa gccaaaaatc
     6841 cgttttattc aacgaatacg gccttattta tggtacctgt aatggatggc aacctcagaa
     6901 aactcatctt gtttctttag cacggaaccc catgaatttt tcccaataaa atatctagga
     6961 ttttgaaacg taaacgaggt gaatctctat ggttcaacca tgtccttccg gtacatttcc
     7021 ctatataata ctacccaggg ataatttcta cattttagcc gaacgctata ataccactgt
     7081 tgaagctatt tcggctgcca atccaggcat cgagccaaac aatctatccg tgggacaaag
     7141 tatttgcatg cccgcaacta aacctccaaa cccttgtcct tgcgggagta gaatttacct
     7201 ggttcaagcc ggggattcgt tgtattcaat cgctcaaaag tataacgttc ctttcgatgt
     7261 gctttacaca gcaaatatcg gtttggatcc ggccaatctc caaatcgggc aggcggtttg
     7321 tattcctcaa ctgcccgcat gtccggctaa ttccgtggct tatgccatag agcccgggga
     7381 ttcgtattac actttatcca aggttacagg tgtccccatt catgatctga tcatcgccaa
     7441 tcccggaatt gatcccaata atcttcaggc cgggcaagtc atatgcctgc cttaccggcc
     7501 aaccccaaca ctttattgac gggctccgag aggcgatggg gaaacagttt acttcttctt
     7561 tttggagttt tcttctaccc taaatttgac gatttttttg atctcgatgg gaaatcgaac
     7621 cgaacccttg gcgttcttat atttcgacaa tataatattt cgtcaagtgt atccactaat
     7681 ttgtcgatat gatttttctc aataattata ggcggtgact atagctctaa cttctaacga
     7741 aacccgcttt accggaacat atccgtgacc ttcacccggg ggtggtggta atatatcccc
     7801 tcggctctgc cccagtgatg aatggccttc aacgggcacc attcagcgca ggcgaagcac
     7861 atctcacact tgtgctgcca aaccggccgc ttatcaacga ttattatgtt ttgtacgggg
     7921 caaaccttgg cgcaagtcgt acatcccata catttgtcat caacatagat gcttctatcg
     7981 gtcaatggga ttaattcgta gtattttaat tttgtgtcct ccggttcctt ggcatacttt
     8041 ttcagggaaa caatgcatgt tccccgaatc aataaacata tcggagtgac caacaagtta
     8101 atgagtgaat ggagtgctcg atatttattg agcttccgtt cagatatgca ttggcaaatc
     8161 gtttcaattt ttttttggca ttttttgaac atgatctctt gatctttatt aatggggacc
     8221 gggatatgag catagtccag gttattcatg ggaagccgga tcgaaaattc cgcagacagc
     8281 ttgccgccca atgatctgat taatctggcc agatttttta ggggcggtag ggcgttgaca
     8341 ctccggtaac cgccgcaggt acagatggcg aaaatgtatt tcgtaccgat atcgtccagt
     8401 tgcttcacaa atttttccac aataaggggt atgccgtaca gttgcgccag ataagacgga
     8461 aacacaaggc cgatacagtc tgcgtccgtt ttgatccttt gtttgttcac gatcgttgga
     8521 atggagatca atctgccttt agtctttgca gcgatatccc ttgccacaac cagagagttt
     8581 cccgtaccgg agaagtagta aatttccatc attacgataa aaccctcttc caatcccaat
     8641 ccccatgtta agctaattcc ttcacataac tcttgtctac agcctgattt aaaaccacgg
     8701 tatttttacc ttattacagt attcatccca ggtggcaccg aaaatctttg acaatattgc
     8761 ttcttcttca ggcgcaaata ttctagaacc aatatatacg ataatcccaa tcaaaactcc
     8821 cagccatgtg ttaaataaaa aacctgccca tggaaacaca agcaaggcca cacctgcata
     8881 taacgggtgc ttaactaggg agtaaggtcc atcggtgatc agtttctttt gaggtacttt
     8941 tgttaagatt aaagctaccg accatatcca attgataacc ccgggaatta aaataactat
     9001 cgaaatcacc gccaaagccg tcgatggacc tccaacactg aataaagagg gaaacataat
     9061 attgagaatc aaaccgatta ccaaaaacgg caatgtaaac agccctattt tttttccact
     9121 tccaacaagt actttcaatt tcatgtttct acctccaata ttcttagtat tttagacatc
     9181 gttttcccga gtttccgcgc ctttttatcg ggaaccctgc agttttacga catatcggtc
     9241 ttcactaatg ttgctatcct cactccatcc caaccagaga agattggctg atcagcatga
     9301 atgtttactt acagagaacg cagtaagaaa tgatccattc gcaggaattg tttcatttgt
     9361 agactttcgg ctaattgaat ggatttcgta ttccgtattt cagccatgta cctcggagtt
     9421 aacttttttt cgaaaagata agcaagcgcc gaagcaacga cgatccgggc ataccctttc
     9481 cctcgttctg tttcaagtgt atgtacaccg gcaatttccc atatatcatc atagttttga
     9541 tacacaaaac agatactcct gatgcgattc tccttcagca agccaaacca aacagctccg
     9601 ttattgaaat gccatcttat ttcggattcc gtataaccat ttcgtttgat aatatctatg
     9661 gcttcatcag tgatcacagt attcccggga actattccat ctttaaaaga atcatccata
     9721 cttgaacagg aatatgaaat atacgatttg cctttcgaga tatcaaatcg gtttcttaac
     9781 ttactcaaat ctaaactctc attgagtctc aggatataat tgctgtttgg taaagtttcg
     9841 agcaactcat atttaagccc gtccgctgtt ccatttataa aaatcgcata attcgtgtag
     9901 ggataggtgg cggtatcaaa cgaaagtatt tgagtcggga ttgttacgag caacgcccaa
     9961 tttttagagt cctctgtcaa atcaactgtc agttgatcgc gatacaataa caagtatttt
    10021 aaggtggtga tgtgttttaa gggatcactt tctaagtacg caaccgattt ttcgtaaatt
    10081 gatttttgca tcgatatcct ctagcggtta ttgcctttga caggcttaag cattatgagc
    10141 agtacaccaa ttcttttcat tatacttgca cctcgttttt aagttttttt ggtaaggaag
    10201 ccgttatagg gcctaacggg ttgtccaatt aaaaatagtg cttcgatcta tagaaatccc
    10261 ctcctattgc ttccacaatt tatcagtatt acctgccaat tttcagaata atttgtcggg
    10321 aaatccatcc atatcaactt acaaaatgaa aatgggtaag ttccctcagg gtacaagagg
    10381 tcgagacggt ttcatctcag ccgacaaggt cgtttcttgc gtttaaattt aaacttcggg
    10441 catgaggttt taaaagacaa aaaacggtca gaagaagcgg agcgggca
//
LOCUS       DASVMB010000171         6677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_22631,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000171 DASVMB010000000
VERSION     DASVMB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22631"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VF531_05105"
     CDS             complement(<1..310)
                     /locus_tag="VF531_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX7713378.1"
                     /translation="MDRRTQTKPLTIPPGMLWTLLVVTLLGQDFLNQPHYAAKICGAS
                     GYWTLLIAFVLIIPVIFLIAAFKRRFPKQNLLNASALALGRPLALVANLLYLSLFLIY
                     A"
     gene            complement(288..1784)
                     /locus_tag="VF531_05110"
     CDS             complement(288..1784)
                     /locus_tag="VF531_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7713379.1"
                     /translation="MDLIQSSPKDFILYIRKELNESADLRFESAAAGEVTVIYLSSIV
                     NQREIEEHFLKPLEQVRIDNAPKKPQRNWETFIAAIPAKDRLKREDAQAVLADILRGK
                     TAVHLAGSNLVYTFDTRKVVKRQINVPMFERTLRGPQLAFNESLADNLALIRQGIKSK
                     ELVIEKLTLGTITQTEIAICYLGNLASPALTREVRQRLESFEIDGIPDSGYLEQLITD
                     NPWSLLPLTQSTERPDRLTAGLLNGKAAILTDNSPQVILIPTTANDLYQSPEDYYFGF
                     WLGSFLRFFRIVGNNIAVALPGLYIALLGVNPELLPIRFALTVSGSRMGVAVPLIIEL
                     LIMEVLLEIFQEASLRLPMPVSQVLGIAAGIVLATASVAAGIVSNATMVIVVITAIAS
                     FSGPDYSIGLSWRILKFILVLAAAFLGLFGLTIAGIMILTHAAIQNSFGTPYLAPWSP
                     IDFRGLIDTVIRRPIWWSKRMKLYQPSDLTRFRWKGGRKKPNGQEDTN"
     gene            2115..5198
                     /locus_tag="VF531_05115"
     CDS             2115..5198
                     /locus_tag="VF531_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7713380.1"
                     /translation="MKRRKQGTGMKRKAVKLHHEISGVPQLGQSSEEILKSIIDAFYT
                     VDADWRLTYINYRAEELWHRRREELIGAVLWDMFPESEQTVGWQMHHQAMIKRVPVHW
                     EMFPPNLQIWVDATAYPTSDGGIAVYFRDISERKRAEEEAAKANEKLMKQPQEVLNER
                     RRLFDILETLPMMICLITPDYHTPFANRSFREKFGEANGRRCYEYCCGYTKPCVSCES
                     LVPLRTGQPHHWEVTSPDGETIIDAHDFPFTDVDGSPLILEMDIDITEHRKIQIALEE
                     SEERFRIMADGIPQQIWVTDAAGRMEFVNRSYVEFFGVTLEEVQSRGWQSLVHPADKA
                     AYVREHMSCLREHRTFYAEARVRRHDGVWRWVESNGLPRFSPVGEFLGIAGSSLDITE
                     RKLAEEALSLSEDRFRAFVTASSEVIYLMNPDWSEMWQLHGKGFLADMKKSSNTWLQE
                     YIAPEDQPQVIAVINEVIRTKNIFELECRVRRADGSLAWTLLRAVPLLDENGEIIEWF
                     GAASDITGRKRVEEDALASERELLKVTVNSLGEGVVVINQHEQIIFINKAAAGLIGYS
                     QEKAIGEAANKILYILDDNTSEPIDKISTQVGFKHLILITRDLKEIPISLNSAPIKSS
                     DGHNIGIVIVFSDITEKQKTEQEMLKAAKLESLGILAGGVAHDFNNILAGILANLQLA
                     NLKLQKHQDILKHMESTIEITRKASDLTKQLLTFAKGGDPVKKTIPIANLVKGTVQFA
                     LSGSKIKPEFHLPETLWMVDIDQGQISQVFNNLTINAEQAMPSGGILEIQGENVVIET
                     TGKYIPGQYVKLSVKDHGIGIPAEIIDKIFDPFFTTKKTGNGLGLSTSYSIIKKHDGY
                     LEVESTPGISTIFYILLPVSKNGETMSEVKNEIAASGEAKILLMDDEEAIRNVGGEML
                     ASFGYLVSLAKDGQEAVELYKEAKETGEPYDVVIMDLTIPGGLGGIETMAILRQIDPE
                     IKAVISSGYASDPVMSEYAKYGFSGVVTKPYKFDELLEALNKVIDRKQLPLNFTF"
     gene            5357..5500
                     /locus_tag="VF531_05120"
     CDS             5357..5500
                     /locus_tag="VF531_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713381.1"
                     /translation="MANPGLYAAYQVRYRFGSRLMDGLLRAWMRDGYWAAVVGQGIET
                     IKV"
     gene            complement(5580..>6677)
                     /locus_tag="VF531_05125"
     CDS             complement(5580..>6677)
                     /locus_tag="VF531_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7713382.1"
                     /translation="SYDIVKTWLFTVWNEPGISNTIFGLKSDEAYLNLYKSTYDAVKS
                     ISSEFTFGGPAEFAAYGKDDNWLLNFLMYAKNAGCRPDFVTAHYYDIDLSDEFFRKRK
                     FQNSLWLSPDPDSFGKHLDMLNHELKEIGYGDTPLYITEWNSTTSHKDLLSDTCFKSA
                     YIIKNAIQISSRINGLCYWLLTDLHEEHQLSPQIFHGGLGLFTVNGIRKPSYYALLFM
                     SRLGKNVIYCNDGVIVTENGSDTIVLLYNYHHYSLTYARDIGINVSYTDRYSAFPDKG
                     KKNIDLEFSHLNGKFVITEQYVNRNHGSAFDNFAKMGAIEPMTINDRDFLFNTSVPQI
                     KKSHCTGSPLKISVSLDAFEIRLINIQPIIE"
BASE COUNT         1914 a         1395 c         1625 g         1743 t
ORIGIN      
        1 tagcatagat caagaaaaga cttaagtata ataaattagc tactaaggcc aatggcctgc
       61 ccaatgccaa agcggaggca ttcagcaggt tttgtttggg aaagcgccgt ttgaaggcgg
      121 cgatcaaaaa aattaccgga atgatcaaaa caaaagcgat caagagcgtc caatagccgc
      181 tggccccaca tatttttgcc gcatagtgcg gctgatttaa gaaatcctgt cctaataaag
      241 taacgacgag gagtgtccag agcattcccg ggggaatggt taggggttta gtttgtgtcc
      301 tcctgtccat ttggtttttt gcgcccccct ttccagcgaa aacgggttag atccgaaggt
      361 tgataaagtt tcatccgttt ggaccaccag atcggacggc ggatcaccgt atcgattagt
      421 ccccgaaagt caatcgggct ccagggagcg agatagggag tgccgaagga attttgaatc
      481 gccgcatgag tcaggatcat gattcccgcg atggttaatc cgaataagcc caaaaaagcg
      541 gcggccaaga ccaggatgaa ttttaaaatc cgccaggaga ggccgatact ataatccggc
      601 cctgaaaaag aagcaatggc cgtaatcacc acgatgacca tggtggcgtt ggaaacaatc
      661 ccggcggcca ctgaagcggt ggccagcaca attccggcgg caatccccaa aacctggctg
      721 accggcattg gcaaacggag actggcttcc tggaaaattt ccagcaaaac ttccatgatt
      781 aataattcga tgattaaagg caccgccact cccatccggc tgccggagac tgttagggcg
      841 aaacggatcg gtagtaattc gggattgact ccgagtaaag caatatatag accgggtaaa
      901 gctaccgcga tgttgttgcc tacaatccgg aagaagcgta aaaatgagcc taaccaaaat
      961 ccgaagtagt aatcctcggg actttggtag aggtcattcg cggtcgtcgg tatcaaaata
     1021 acctgggggg agttatctgt taaaatggcg gcttttccat ttaagagacc tgccgttaat
     1081 ctgtccggcc tttcggtgct ttgagttaac ggcaggagcg accagggatt atcggtgatt
     1141 aattgttcca ggtaaccgct atcgggtatt ccgtcgattt caaaggattc taaacgctgc
     1201 cggacttccc gggttagggc tgggctagcc aaattgccaa gatagcaaat ggcaatctcg
     1261 gtttgggtga tcgttcctag ggttaacttt tcaatgacaa gttctttgct tttaattcct
     1321 tgccggatca gggcgagatt gtcagccaga ctttcgttga aagcaagttg ggggccgcgc
     1381 agggtccgtt caaacatggg tacattgatt tgccgtttaa caaccttccg ggtatcaaaa
     1441 gtgtagacca gattgctccc agccaaatga acagcggttt tcccccttaa aatatccgcc
     1501 agtaccgctt gagcgtcttc ccgtttgagc cggtccttgg ccggaatggc agcgatgaat
     1561 gtttcccaat tcctttgagg cttcttaggg gcgttatcta ttcgcacttg ttccagcggt
     1621 tttaaaaaat gctcctcgat ctccctttga ttgacaatgg aggagagata aataacggtt
     1681 acctctcctg cggcggctga ttcaaagcgc agatcggcgg attcgttaag ctctttccgg
     1741 atataaagga taaaatcttt aggggatgat tgaataagat ccaaattgaa actccatttg
     1801 atgaataagt gaaaaatttc tttatcttta tggtaggttg atggactgct ttttatcata
     1861 aaatagaacg attttttaaa agtaaaaaat ggaatgaatc ccatatatag gcaaggggca
     1921 atacgttttt accaaccggg caaatcagtt gaagacgaaa atctttaaca ccaaatatat
     1981 tcttggatgt ttctccagat atttgtcgaa agtctaacaa cataagacag attatcagga
     2041 aaaagtttat ggtttattgt attatcaatt tctattcgcg aatttatcaa tcttttcccc
     2101 ggggacaagc agaattgaag cgaagaaagc aaggaaccgg catgaaaagg aaagccgtca
     2161 aactgcatca tgaaatttcg ggagtacccc aattagggca aagcagcgag gaaattctga
     2221 agagcatcat cgacgccttt tataccgttg acgccgattg gcgcttgact tacattaact
     2281 atagggcgga ggaattgtgg caccgccgcc gggaagagtt gataggggct gtactttggg
     2341 acatgttccc ggaatctgag cagacagtcg gctggcaaat gcaccaccag gctatgatca
     2401 agcgtgttcc cgtccattgg gagatgttcc ctcctaattt gcagatttgg gtggatgcta
     2461 ccgcctatcc cacatcggac ggcggtattg ctgtttattt ccgcgatatc agcgagcgta
     2521 agcgggccga ggaagaggcc gcaaaagcga atgagaagtt gatgaaacag cctcaggaag
     2581 tcttgaatga acgccggcgc ttgttcgaca tcctggaaac cttgccgatg atgatctgcc
     2641 taatcacccc ggactatcac actccttttg ccaaccgatc ctttcgggag aaattcggtg
     2701 aagccaacgg ccggcgttgt tatgaatatt gctgcgggta tactaagccc tgcgtgtctt
     2761 gcgaatcgct ggtgccgctt agaaccggcc aaccgcatca ctgggaggtt acttctcccg
     2821 atggcgagac catcatcgat gcccatgact tcccattcac cgatgtggat ggttcgccgc
     2881 tgattctgga gatggatatc gatattaccg aacacaggaa gatccaaata gcgctggaag
     2941 aaagcgaaga gcgattcagg atcatggcgg acggcattcc gcagcaaatc tgggtcactg
     3001 atgcggcagg ccggatggag ttcgtcaatc gaagctacgt ggagtttttt ggtgtcacgt
     3061 tagaggaagt ccaatcccga ggctggcagt ccctggtgca tcccgccgac aaggctgcct
     3121 atgtccggga gcatatgtcc tgtctgcgcg aacaccggac tttttacgcc gaggctcgcg
     3181 tccggcggca tgatggggta tggcgttggg ttgaatctaa cgggctgccg cgcttctcgc
     3241 ccgtcggaga atttttgggc atcgcgggca gcagcctcga catcaccgag cgcaagctgg
     3301 ctgaggaggc gctgagctta agcgaggatc gtttccgtgc ctttgtaacc gccagttcgg
     3361 aagtgatata tcttatgaat ccggattgga gcgagatgtg gcagcttcat ggcaaaggct
     3421 tcctcgccga tatgaaaaag tcaagcaata cctggctcca ggaatatatt gctccggaag
     3481 atcaacccca ggtgatcgcg gtcattaatg aagtcatccg gacgaagaac atctttgaat
     3541 tagaatgtcg agtccggcga gcagacggca gcctggcatg gactttatta cgcgcggttc
     3601 cgctgttgga tgagaatggc gaaattattg aatggtttgg cgcagccagt gacattaccg
     3661 gccgcaaacg ggttgaagaa gatgctttgg cttccgaaag ggaattattg aaggttactg
     3721 tgaactctct tggcgaagga gtagttgtta ttaatcaaca tgaacaaatc atttttatca
     3781 ataaagcagc tgccggtcta atcgggtatt cgcaggaaaa ggcgattggc gaagcggcca
     3841 ataaaatttt gtatatattg gatgataata cgagtgaacc aatagataaa atttccacgc
     3901 aagtgggatt taaacacctt attttaataa cccgggattt aaaggaaatt ccgatatccc
     3961 ttaacagcgc acccattaag tcatctgatg gtcataatat cggtatcgtg attgtttttt
     4021 ccgatatcac cgaaaaacaa aaaaccgagc aggaaatgtt gaaagccgct aaacttgaat
     4081 ctttaggaat tctcgccgga ggagtcgccc atgattttaa caatatcttg gccggaattt
     4141 tagccaatct gcaactggca aaccttaagt tgcagaaaca tcaagatata ttgaaacata
     4201 tggagagtac tattgaaatc acacgtaagg ccagcgatct caccaaacaa ctgctgactt
     4261 tcgccaaagg tggcgaccct gttaaaaaga ccattccaat tgccaatctg gtcaagggta
     4321 cggtccagtt cgctttgagc ggttccaaaa taaagcccga gtttcatctc ccggagactc
     4381 tctggatggt ggatatcgat caaggacaaa tttcgcaagt gtttaacaat ttaaccatca
     4441 acgctgaaca ggcgatgccc agcgggggca tccttgaaat tcaaggcgaa aatgtggtta
     4501 ttgagaccac cggcaaatat attcccggcc agtatgttaa attgagcgtc aaagaccacg
     4561 gcatcggtat tcctgcggaa atcatcgata aaatctttga cccgtttttt accaccaaaa
     4621 aaaccggcaa cggcctcgga ctctcgacct cctattctat aatcaaaaag catgacggtt
     4681 atttggaagt ggaatccacg ccgggaatca gcactatctt ttatatctta ttgccggtat
     4741 ccaaaaacgg agagaccatg agtgaagtta aaaatgaaat tgctgctagc ggggaagcaa
     4801 aaatattgct gatggatgac gaagaggcca tccgtaatgt cggcggggaa atgttagcca
     4861 gttttggcta tctggtttcg ttagctaaag atgggcagga agcggttgaa ctttataaag
     4921 aggctaagga aaccggtgag ccatatgatg tagtgattat ggatttgact atccccggcg
     4981 gtttgggcgg tatcgaaacc atggccatct tgcggcagat tgatccggag attaaagccg
     5041 tcatctccag cggttatgcc agcgacccgg tgatgtccga atatgcaaag tatggtttta
     5101 gcggagtggt caccaagcct tataagtttg atgaattact tgaagcatta aataaggtga
     5161 ttgatcggaa gcaattgccg ttgaatttta cattttgata taataatgta aataaaattc
     5221 cgatattgaa aacgggctgt tcgtatttgg cgattgaact tggagaaaat cttgcagagt
     5281 atacaaagaa taaattctaa tgtttgggta caacatgaat tttagcagtg tcaagtaaga
     5341 gacgggttgg ccttccttgg ccaaccctgg gttatacgcc gcatatcaag tccgatatcg
     5401 cttcgggtcg cggttgatgg acgggctgtt gcgcgcatgg atgcgcgacg gatattgggc
     5461 ggctgtggtg gggcagggaa ttgaaacgat aaaagtgtga cagggtaaga gaaaatagca
     5521 aagagaaaag ggaaaaagtg cttaccaatg gcctgaaagt tattacagat aattggaatt
     5581 tactcaataa tcggttggat gttgatcaac cgtatctcaa acgcatctaa agaaacactg
     5641 attttgagcg gagagccagt acagtggctt ttttttattt gaggaacact cgtattaaat
     5701 aaaaaatctc tatcattgat ggtcataggt tcaattgcac ccattttggc aaaattgtca
     5761 aatgcactgc cgtgatttcg atttacatat tgctctgtaa ttacaaattt accgtttaga
     5821 tgtgaaaatt caagatcaat attttttttg cctttatcag ggaacgcaga atatcgatct
     5881 gtataagaaa cattaatacc gatgtctcga gcataggtaa gactataatg atgataattg
     5941 taaagaagta caatagtatc gctaccattt tcagtaacaa taactccgtc gttgcagtaa
     6001 ataacatttt ttccgaggcg actcataaag agcagtgcat aataacttgg ctttcgaata
     6061 ccattgacgg taaataaacc aagtccaccg tgaaaaatct gcggactaag ctgatgttct
     6121 tcatgtaaat cggtaagcaa ccagtagcaa agtccgttta ttcttgagga tatctggatg
     6181 gcgtttttta ttatataggc ggatttaaag caagtgtcgg ataataaatc tttatgagaa
     6241 gtggttgagt tccattctgt tatatataac ggcgtatcgc cataaccaat ttcttttaac
     6301 tcatggttaa gcatatctaa atgctttcca aatgaatcgg gatccgggct caaccataac
     6361 gaattttgaa attttctttt tctgaaaaat tcatcggata agtcaatatc ataatagtgg
     6421 gcagttacaa aatcaggacg acaaccggca tttttggcat acataagaaa attcagcagc
     6481 caattatcgt cttttccata agccgcgaac tcggcaggac caccgaaggt aaattcgcta
     6541 ctgatagatt taactgcatc atatgtggat ttatataaat ttaaatatgc ctcatcactt
     6601 ttcagcccaa atatcgtatt tgatattcca ggttcattcc atactgtaaa taaccaagtc
     6661 tttacaatat cataaga
//
LOCUS       DASVMB010000172         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_316289,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000172 DASVMB010000000
VERSION     DASVMB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316289"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VF531_05130"
     CDS             complement(<1..483)
                     /locus_tag="VF531_05130"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX7713383.1"
                     /translation="MKVALAEQMRVIDQRTQDEYGIPGVVLMERAALALVDEARDLLG
                     ELAGKRIYLYCGKGNNGGDGLAAARLFLETGAIVTGVLLFPQGQYRGLAAENLKRAST
                     YGVSLRDWETVSDQELEYCDLIIDAILGTGTVGAATGVAAAAIERINRSGRPVLAVDL
                     P"
     gene            complement(446..850)
                     /gene="acpS"
                     /locus_tag="VF531_05135"
     CDS             complement(446..850)
                     /gene="acpS"
                     /locus_tag="VF531_05135"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7713384.1"
                     /translation="MIAGNGIDIIEIDRIAAASRNSRFNNRVFTPGELQESKELPQHL
                     AGCFAAKEALLKSLGTGLAGFSWLEMEVHHDERGAPYFQVSGKIRSYLEKTGITRIHL
                     SISHSQLYAIAQVILETEDRHEGCTSGTDAGH"
     gene            complement(864..1661)
                     /locus_tag="VF531_05140"
     CDS             complement(864..1661)
                     /locus_tag="VF531_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX7713385.1"
                     /translation="MRDRRRERYLDYLTFKKLAGELTGIDLADYKSTQMDRRINSLKQ
                     SWNLETYDDFLTLLKTDSQRYKEFVKKLTINVSEFFRNPERFIELWQQILPELLAKRP
                     VVRVWSAGCSNGSEPYSIAIIAHELHAAERVQILATDVDQQILEKARQAVYMPNEVRS
                     LPPELLAKYFTRQDELYCLNEEIKHGIRLGFHNLLLDPFETNLDLIICRNVVIYFTEA
                     AKNTLYRKFLDALRPGGYILVGGTEPLLQYRQMGFENPLTSFYKKPE"
     gene            complement(1808..>2062)
                     /locus_tag="VF531_05145"
     CDS             complement(1808..>2062)
                     /locus_tag="VF531_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7713386.1"
                     /translation="SDDFFRTMEIQSSAAAVENLLLAATSLGLATVWLGILFLIKDDV
                     LKFLNEPAGEFMAVIPVGYPSRSTQGPQKRPLDLIVKRLD"
BASE COUNT          505 a          561 c          500 g          496 t
ORIGIN      
        1 aggtaaatcc acagcaagta ccggccggcc ggagcgattg attcgttcaa tagccgcggc
       61 ggcaactccg gttgccgctc ctaccgtacc cgttcctagt atggcatcga tgatcaggtc
      121 acagtattcc agttcttgat cgctgactgt ttcccaatct ctaagactta ccccatatgt
      181 ggaggcacgt ttcaggttct cggcggccaa accgcgatat tgaccctgcg ggaagagaag
      241 cactcccgtg acaatcgcgc cggtttccag gaataaccgg gcggcggcaa ggccgtcacc
      301 gccattattg cctttaccgc aataaagata gatccgtttt ccggcgagtt cgcccaggag
      361 atcccgggcc tcatcgacca aggccagggc ggcccgttcc atgagcacta ctcccgggat
      421 accgtattcg tcctgggtcc gctggtcaat gacccgcatc tgttccgcta gtgcaacctt
      481 catgtctatc ctcggtttcc aaaatgacct gggcaatggc atacaattgc gaatgggata
      541 tactgagatg gatccgtgtt atcccggttt tctccagata ggatcggatc ttgccgctta
      601 cctggaagta gggggctccc cgttcatcat gatgtacctc catctccagc caggaaaaac
      661 cggccaaccc ggtaccgaga gattttaaca aggcttcctt ggctgcgaaa caccccgcca
      721 gatgttgggg gagttcttta ctctcctgta attcacccgg ggtaaaaacc cggttattga
      781 accggctgtt ccgggaggcc gcagcaattc ggtcgatctc gataatgtcg attccgttgc
      841 cggcaatcac atgaaacgcc tccttactcc ggctttttat aaaacgaagt aagcggattt
      901 tcgaatccca tctgccggta ctgaagtaag ggttcggttc ccccgaccag aatgtatccg
      961 cccgggcgaa gggcatccaa aaacttccga taaagagtat tcttcgccgc ctcggtgaaa
     1021 taaatcacta cattgcggca gataatcaga tctaggttgg tctcgaaggg atctaagagc
     1081 aggttgtgaa atcccaaacg gatcccatgc ttaatctctt cattcaggca atagagttca
     1141 tcctggcggg tgaaatattt ggcaagcaac tccgggggca ggctacggac ctcattgggc
     1201 atataaacag cctgacgtgc tttttccagg atctgctgat cgacatcggt ggccaaaatc
     1261 tgaacccgct ccgcggcgtg aagctcatga gcgataattg cgatggaata aggctcggat
     1321 ccgttggaac atccggcgct ccaaacccgg accaccggac gcttggcaag taactccggg
     1381 aggatctgct gccagagttc gataaaacgc tcgggattcc gaaagaactc cgaaacatta
     1441 atggtcagct ttttaacgaa ctccttatac cgttgcgaat cagtctttaa caaagtcaga
     1501 aaatcgtcat aggtttcaag attccaactt tgttttaggg agttgatcct tcgatccatc
     1561 tgggtgcttt tataatctgc caaatcgatc ccggtcagtt caccggcgag cttcttaaaa
     1621 gtcagatagt ccaaataacg ttccctccgc ctatcgcgca aacccaaacc tgccccggaa
     1681 gcccggcaat atcatccacc tgatttgcgc gcgcctattc ttgctatcat atgaagtatt
     1741 ctatccgtga aacggcgaat ccttcaacaa taccagaagt tcgcacagtt tctagtggtc
     1801 cggtttctca gtcaagccgt ttgacgatta gatccagtgg ccgtttctga ggcccttgag
     1861 tggatcttga cggataaccg acaggaatca ctgccataaa ttccccagcc ggttcattca
     1921 agaattttaa aacatcgtct ttaatgagga agaggattcc cagccagaca gttgccaggc
     1981 caagggatgt agccgctaaa agcaggtttt ccaccgcagc ggcagagctt tggatctcca
     2041 tggtccgaaa gaaatcatcg ga
//
LOCUS       DASVMB010000173         8286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_538563,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000173 DASVMB010000000
VERSION     DASVMB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538563"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF531_05150"
     CDS             complement(<1..237)
                     /locus_tag="VF531_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HEX7713387.1"
                     /translation="MATPIIMPRQGQSVESCIIAKWYKKKGDPVKAGDILFSYETDKA
                     AFDEEAKLDGVVLEIFCGEGDDVPVLTNVCVIGNP"
     gene            complement(377..1642)
                     /locus_tag="VF531_05155"
     CDS             complement(377..1642)
                     /locus_tag="VF531_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7713388.1"
                     /translation="MRSKALRMYGKSDLRLEEFELPRLRDDQILAHIFSDSICMSTYK
                     AAIQGSDHKRVPKNIAGHPVIIGHEFCGELVEVGDKWRDQFQPGQKFTVQPALNQQDD
                     PYAAPGYSFEYIGGAATYVVIPNQVMELGCLLRYDGDAYFYGSLAEPISCIIGAFHAN
                     FHTTPGSYIHQMEIAPGGNMAILAGVGPMGLGAIDYAIHGPLKPRLLVVTDLDQARLD
                     RAGSIHTLQEAAKNGVTLKYINTGTLANPAADLLQITGNKGFDDVFVFAPVKTVVEMG
                     DRILGKDGCLNFFAGPTDPNFSATINFYNTHYASTHVAGTSGGNTDDMVESLHMMEKN
                     LINPAGMITHIGGLNCAARTTLNLPKIPGGKKLIYTNIKLELTAIDDFQTAGETQPLF
                     AELAGIVAKSNGLWSTEAEKYLLGHAETI"
     gene            1934..2725
                     /gene="srlD"
                     /locus_tag="VF531_05160"
     CDS             1934..2725
                     /gene="srlD"
                     /locus_tag="VF531_05160"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HEX7713389.1"
                     /translation="MNNPTRLEGQVAIVTGAAQGLGKALAERLAEEGCRVVAADINFE
                     AAQKVASNLHRGIAVKVDVCDEQQVESMVDTAVREFGRLDLLVSNAAILIAKPVLEFP
                     VEQWRKMMEINLVGYFLAAKAAARVMIPNHKGTIIQINSKSGKKGSYKNSAYAAAKFG
                     GIGLTQSLALELAEYNIRVNAICPGNLLDSPLWNEGPNCLFEQYAHNQGITVAEVRQK
                     YLSQVPLGRSCSYDDVANVMVFLASDQAGYMTGQAINVTGGQEMR"
     gene            2782..4128
                     /locus_tag="VF531_05165"
     CDS             2782..4128
                     /locus_tag="VF531_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7713390.1"
                     /translation="MLNKYQIYFTGLNHPEPSERLENLRRLAEMVAAGEIGQPQRGVD
                     VNNHIHTIYSFSPYSPAKAIWMAYQSGLTTAGIMDHDSISGALEFVEAGRIVGMATTI
                     GVECRSDFSGTHLKGRRINNPDQPSVAYVALHGIPHTKIDEVKRFFAPYLEERNIRNR
                     QMVERINDILKSSGVKLDFTNDVLAISSFAEGGSVTERHILYALAGKFIERYGKGESL
                     VGFLKNQLGLNLSPKLENFLGETANEFYQYDLLSGLKSDLVSAFYIDATRECPDIKRL
                     IEFAGEIGAIPAYAYLGDVEASVTGDKKNQKFEDDYLDFLFEVLGELGFKAVTYMPTR
                     NSASQLERVRNLCRRYGFFQISGEDINSPRQSFICNALRDEKYQNLVASAWALIGHEL
                     SATEDVGRGLFTPDTTRRYPDLNDRIKAYEAIGKQKVKIFIRPRAVNREMDRDVCN"
     gene            4115..4882
                     /locus_tag="VF531_05170"
     CDS             4115..4882
                     /locus_tag="VF531_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7713391.1"
                     /translation="MFAIERRNHIMTLLNELKSLSVQEMAVNLCVAEETIRRDLKELE
                     SLGLLIRTHGGAVLADDSKTEVSLAHREGINVFGKDIIGKEAAAMVKDGDTIILDAST
                     SALSVAKHLKDKQGLTVITNAEKVVFELAGQDEITVISTGGTLRHQSLSYVGRAAVEA
                     LSKYYANRVFFSCKGLTRNRGLTDSNEQEAEIRRTMIQNSEQVVFLCDYTKFGKVGYV
                     NTVGLEAVDMILLDRPLPEEWQAEIAAKGIRAKVAEG"
     gene            complement(4953..6239)
                     /locus_tag="VF531_05175"
     CDS             complement(4953..6239)
                     /locus_tag="VF531_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7713392.1"
                     /translation="MNFDLLHPADQIVMIMERIYGYGMTTTSGGNLSILDDNGDIWIT
                     PGGIDKGSLTRNDIIRVKPDGTIVGNHKPSVELPFHQQIYQRRLNIKAVLHAHPPALV
                     SFSIARKKPNTALIPHADLICGQVGMAEYGLPGSEELGENIAAVFSKGYDTVMLENHG
                     VVVGHENLFKAFMAFETLDFCARLEIEANRLGKPIALSPQNIALSHQKQHTRIPEFVS
                     HHYSSEEKQARRDMCQLIHRAYDQQVFTSTQGTFSQRLTDHSFIITPYMVDRKYIEPG
                     EIVRIENNMREANKTPSRSVMLHQYIYEKHPHIHSIIIAHPPNIMAFAITDVLFDSRI
                     IPESYILLRNVPKLPFGSSFMEPQKTAEVFTKNTPLALVANDCVIVTGSSLINAFDRL
                     EVAEYSAKAIIASRSIGNVVMIDNERIAEIEKAFPL"
     gene            6449..7510
                     /locus_tag="VF531_05180"
     CDS             6449..7510
                     /locus_tag="VF531_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7713393.1"
                     /translation="MVQQELVELQQISTAVGIPIDYTQGGGGNTSVKLGNQLMAVKAS
                     GFKLKQITPGEGYVLVNYQKILDFYHQIDPDRMNDLEKESGQLLKDSVVIKDTMKPLR
                     PSVEAGFHSLLKKYVIHTHPVYANILCCAQNGRELAQRIFGGKSYGWVWIPYVNPGFS
                     LTLKIKAAIEEALERKGEFPQVIFMENHGLIVNADDCTNCIILHQAVNDLIRDYLGIK
                     GPYPQIELVAGDDQTIISKTPYIMDYFKGSKLSDDFFDEVVLYPDQLVYLNGEISTGR
                     MDRKVNIHLQSGTVTYKTGLAEAQTIEETLLAYLYVIKGIRSHGLQIKTMTSQEIGFI
                     KNWESEAYRKRLTKEATAK"
     gene            7586..>8286
                     /locus_tag="VF531_05185"
     CDS             7586..>8286
                     /locus_tag="VF531_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7713394.1"
                     /translation="MSQYILELQGITKTFPGVTALSDVHFQLRRGEIHALMGENGAGK
                     STFIKIITGVHQPDGGEIYLNGKKTVIKSPNDAQSLGIAAIYQHVTCYPDLTVTENIF
                     MGHEQVQRFTGRILWQQMHREAKTLLTGLGVEIDPRTRMGALSVAQQQTVEIAKALSM
                     RAKIIIMDEPTSALTKRESEELYRITEQLRDNGASIIFISHRMEDMYRLADRVTVLRD
                     GQYIGTWEAEGISGD"
BASE COUNT         2198 a         1860 c         2157 g         2071 t
ORIGIN      
        1 gggattgccg atgacgcaaa cattggttaa gaccggtaca tcatcgccct cgccgcaaaa
       61 tatctccagt acaacgccgt cgagttttgc ctcctcgtca aaagccgcct tgtcagtctc
      121 ataggaaaaa agaatatcac cggccttgac cggatcgccc ttttttttat accatttggc
      181 gatgatgcag ctttcaacgg attggccctg gcgcggcata attattggtg tagccatggg
      241 tgacctcctg atatcaaaat tggcgttcca gtgcgagacc catatcatca tgattctttt
      301 ttgtctaagg gtatctccgg tgtgagcgtg ccatgtttga aactaacaaa gagtataagg
      361 agtgccttgt cgggcatcaa atcgtttcgg catggccaag cagatatttc tcggcctccg
      421 ttgaccataa accgttactt ttggcaacaa tcccggccaa ttccgcaaat agaggttgag
      481 tctccccggc tgtttgaaaa tcatcaatgg ccgtcaattc cagtttgatg ttggtgtaga
      541 ttaacttttt gccgcccggt atttttggca ggttcagcgt agtccgggcc gcacagttta
      601 aaccgccgat atgggtgatc atccccgcgg gattgattaa atttttctcc atcatatgga
      661 gggattcgac catatcatcg gtattaccgc cactggtacc ggctacatgg gttgaggcgt
      721 aatgcgtgtt atagaagtta atcgttgcgg aaaagttggg gtcggtcgga ccggcaaaga
      781 aattgagaca accatccttc cccaaaatcc gatcccccat ttccactact gtctttacgg
      841 gggcaaaaac aaagacatca tcaaaacctt tatttccggt aatctggagc aggtcggcgg
      901 ctgggtttgc taaggttccc gtgttgatgt attttaacgt gactccgttt ttagcggctt
      961 cctgtaatgt atggattgaa ccggcacggt ccaggcgggc ctggtcgaga tcggtaacca
     1021 ccagtaatct gggtttcagc gggccatgga tggcataatc gatcgccccc aatcccatcg
     1081 gacccactcc ggccagaatc gccatgttgc cgcccggcgc aatttccatt tgatggatgt
     1141 aacttccggg agtggtgtgg aaattcgcat ggaaagcgcc gataatgcac gaaatcggtt
     1201 cggccaagga gccgtaaaaa taagcatcac cgtcataccg gagtaagcat ccgagttcca
     1261 tgacttgatt gggaataaca acataggtgg ctgcgccgcc aatatattca aaggaatatc
     1321 cgggagccgc gtaagggtcg tcctgctgat tgagcgcggg ctgaaccgta aatttttgac
     1381 cgggttgaaa ttgatctctc catttatcgc caacctcaac caattctcca cagaactcat
     1441 gtccgataat taccggatga ccggcgatat ttttcggcac tcttttgtga tcgctgcctt
     1501 gaatcgccgc tttataggtc gacatacaga tgctgtcgga gaagatatgg gccaaaatct
     1561 ggtcatcccg cagccgggga agttcaaatt cctccagccg caaatccgat tttccataca
     1621 ttcgaagggc tttgcttctc atttcaattc cccctagaac cgaatgattg tgagatattt
     1681 tcatcgtttg tcactgttaa acatcataaa cgaatcatct cacaattccc caaaaagtcg
     1741 aatggctcac ggctcaaatg aaattgaatc gtagataaat ggcagattca tcaaccggta
     1801 caaacataat cggaacaata tagacacaaa accacatatt atgtataatg tgattataac
     1861 acgaatatgt tacttttgaa cattattttt ttaatctttc cattgacctt aagaggaggc
     1921 ttgcaaaatg gggatgaata atccaacccg tttggaggga caagttgcaa ttgtcaccgg
     1981 ggcggcccag ggtttgggaa aagccttggc ggagcggctt gcggaggaag gttgccgggt
     2041 ggtggcggcg gatattaact ttgaagccgc tcaaaaagtg gcaagtaatc ttcaccgtgg
     2101 gatagccgtt aaggtggacg tttgtgacga acaacaagtg gaatccatgg ttgatacggc
     2161 tgtccgggag tttggccggc tggacctgct ggtctccaat gcagccatac tgattgccaa
     2221 accggttttg gagtttccgg ttgaacagtg gcgtaagatg atggagatca atctggtggg
     2281 ttacttcctg gctgcaaagg ccgcggccag ggtgatgatt cccaatcata agggaaccat
     2341 cattcaaatc aacagtaaat ccgggaagaa aggatcctac aaaaattcgg cctatgccgc
     2401 ggccaaattc ggaggtatcg gattgaccca gagtttggcc ttggagttgg cggagtacaa
     2461 tatccgggtt aacgccatct gtcccggtaa tttactcgac tctcccctgt ggaatgaggg
     2521 accgaattgt ctgttcgagc agtacgccca taatcaagga atcactgtgg cagaagtgcg
     2581 ccaaaagtat ttaagtcagg ttcctctggg cagaagttgc agttatgatg atgtcgccaa
     2641 tgtcatggta tttttggctt cggatcaggc cggctatatg acgggacagg cgattaatgt
     2701 taccggtggt caggagatgc ggtaaaaggt gttgacagtt taggggccca tgaaggtatt
     2761 gacaggggag tgggagtata tatgctcaat aaataccaaa tatacttcac gggtttgaat
     2821 cacccggaac cttcggaacg gttggagaat ttacgccggc tggcggagat ggttgcggcc
     2881 ggtgaaatcg gtcaacccca aagaggagtt gatgtaaata accatattca taccatttat
     2941 tccttctcgc cttactctcc ggccaaagcc atctggatgg cctaccagtc cggcctgact
     3001 acggccggaa tcatggatca cgactcaatc agcggtgctt tggaatttgt agaggccggc
     3061 aggatcgtcg ggatggcgac cacgatcggc gtggaatgcc ggtccgattt ttcgggcacc
     3121 cacctaaagg ggagaaggat taacaatccg gatcaacctt ccgttgccta tgtggccttg
     3181 catggcattc ctcacaccaa gatagacgag gtgaaacgct tttttgcccc gtatctcgaa
     3241 gaacggaata ttcgtaaccg ccaaatggtg gaacggatca atgacattct aaaatcatcc
     3301 ggggtaaagc tggattttac caatgatgtc ctggctatct ccagttttgc cgagggcggc
     3361 agcgtgacag aacggcatat cctctacgcc ttggcaggta aattcattga aaggtatggc
     3421 aaaggagaat cactggtcgg attcctaaaa aatcaacttg gtttaaacct cagccccaaa
     3481 cttgaaaact ttttaggaga aaccgccaat gaattttatc aatatgatct gttaagcggg
     3541 ttgaaaagcg atttagtaag cgcattttac attgatgcca cccgggaatg cccggacatc
     3601 aaaagattga tcgaatttgc cggtgagatc ggcgcgattc ccgcctatgc ctacctgggg
     3661 gatgttgagg cttcggtgac cggggacaag aagaatcaga agtttgaaga cgattatctg
     3721 gattttttgt tcgaggttct cggtgaattg ggttttaagg cggtgacgta tatgccgacc
     3781 cgtaactctg caagtcagct ggaacgggtg cgcaaccttt gccggcgata tggattcttt
     3841 cagatcagcg gggaagatat taattctcca aggcaatcct ttatttgcaa tgccttacgg
     3901 gatgaaaagt atcaaaatct ggtggcatcc gcctgggcgc tgattggtca tgaactgtcg
     3961 gcaaccgaag acgtgggtcg gggattattt acgcccgata ccacccggag ataccccgac
     4021 ctgaatgatc gaatcaaggc ctatgaagcc atcggtaagc aaaaggtaaa aatatttatc
     4081 cggccgcggg ccgtaaaccg ggagatggat agggatgttt gcaattgagc gccgcaacca
     4141 tatcatgacc ttattaaacg agctgaagag tttatcggtt caggagatgg ctgtcaacct
     4201 ctgcgtcgcc gaagaaacga taagacgcga tctaaaggag cttgagagcc tgggattatt
     4261 gatcaggact catggcggag cggttttggc cgacgattcg aaaaccgagg tttccttggc
     4321 gcatcgagag ggaatcaatg tctttggtaa ggatatcatt ggtaaagagg ccgccgctat
     4381 ggttaaggac ggcgatacga ttattctcga tgcaagcact tccgccttgt cggttgccaa
     4441 acatttaaag gacaagcaag gcttaacggt gatcaccaac gcggaaaaag tggtttttga
     4501 attggcgggc caggatgaga tcacggttat ctccaccggc ggcacgcttc gccaccaaag
     4561 tctctcgtat gtggggcgcg cagccgtaga ggctttgagt aaatactatg ccaaccgggt
     4621 ctttttttcc tgcaagggat tgacccgtaa ccggggcttg accgactcca atgaacagga
     4681 agccgagatc cggcgaacca tgatccaaaa ttccgaacag gttgtgtttt tatgcgatta
     4741 taccaaattc ggcaaggtgg gttatgtaaa caccgtaggt ctggaagcag ttgacatgat
     4801 cttgcttgac cggcctttgc cggaggagtg gcaggcggag attgccgcca aagggattcg
     4861 agcgaaggtc gccgaaggct aaaccgcgat ccggcaagtt cttatttgaa acgaattgaa
     4921 agttgcttgc cggattcgtg ttacggcccg aatcataatg gaaaggcctt ctcaatctcc
     4981 gcaatccgtt cattatcaat catcaccaca ttaccgatgc ttcgagaggc aataattgct
     5041 ttggcgctgt attcggccac ttccaaacgg tcgaaggcat taatcagact actcccggta
     5101 acgataacac aatcattggc gaccaaagcc aggggcgtgt tcttggtgaa cacctccgct
     5161 gtcttttggg gttccatgaa gctcgaaccg aaaggcagtt tgggaacatt gcgcagcaaa
     5221 atatagctct cgggaatgat ccgggaatcg aaaagtacgt ccgttatggc aaaggccatg
     5281 atattcgggg gatgagcgat gatgatcgag tgaatatggg gatgcttttc ataaatatac
     5341 tggtggagca tgaccgaacg gcttggcgtt ttattcgctt cgcgcatatt gttttcaatg
     5401 cgaacgatct ctccaggctc tatgtatttt cgatcaacca tatagggcgt gataataaag
     5461 gagtgatcgg tcagccgttg agaaaaggtt ccctgggtac tggtaaagac ttgctgatca
     5521 taggcgcggt gaatcaattg acacatatcg cggcgagcct gtttttcctc actggagtag
     5581 tgatggctga caaattcggg gatccgggta tgctgctttt ggtgggataa ggcgatattt
     5641 tgaggagata aagcgatcgg tttgccgagc cggttggctt cgatttcgag acgggcgcag
     5701 aaatcgagag tttcaaaggc cataaaggct ttgaaaagat tttcgtggcc tacgacaact
     5761 ccgtgatttt ccaacatcac cgtgtcatat cccttactga acactgcggc aatattttcc
     5821 cccaattcct cacttccggg caggccgtac tcagccattc cgacttggcc gcatattaaa
     5881 tcggcatggg ggatcaaagc ggtgttgggt ttttttctgg cgatgctgaa ggaaaccagc
     5941 gccggcggat gggcatggag gaccgctttg atattcaatc ttctttgata gatctgttgg
     6001 tgaaagggaa gttcaaccga gggtttatgg ttgccgacga tggtgccgtc gggtttgacc
     6061 cggatgatgt cgttacgggt cagtgaacct ttgtcaatac cgcccggcgt aatccagata
     6121 tctccgttat catccaaaat ggagagattt cctccggaag tagtggtcat accgtatccg
     6181 tagatccgtt ccataatcat gacaatttga tcggcgggat gtaaaaggtc gaagttcatt
     6241 atctcacctc tgcgctttat tgtgcttcac taagagttga ccaaaaccaa aagttcacca
     6301 aaactcaacc aaccggagga atcgcataat ccttcaaatg atccatataa ataataacat
     6361 ataccaatac tttacgaaaa cagtattaca aaatatttat gtcggtttta aatcgttttt
     6421 attaacatat taagggggcc tttcgaacat ggttcaacaa gagttggttg aattgcaaca
     6481 aatatcgacg gcggtgggta taccgatcga ttacacgcaa ggcggcggcg gcaatacttc
     6541 ggttaaattg ggcaaccaat tgatggcggt aaaagcctcc ggattcaaat taaaacagat
     6601 tacccccggt gagggatacg tattggttaa ttatcaaaag attctggact tttatcatca
     6661 gattgatccg gaccgcatga atgacttgga gaaggaaagc ggccaactgc ttaaggacag
     6721 tgtggtaata aaagatacga tgaaaccctt gcgcccttcg gtggaagcag gttttcactc
     6781 attgcttaaa aaatatgtca tccatactca cccggtttat gccaacatcc tgtgttgtgc
     6841 ccaaaatggc cgggaacttg cgcagaggat ttttggcggc aaaagttacg gctgggtttg
     6901 gatcccatac gtaaacccgg gtttttctct tacgctaaag attaaagcag ccatcgaaga
     6961 agcgctggag cggaagggcg agtttcccca ggtcatcttc atggagaacc acggcttgat
     7021 cgtcaatgcc gatgattgta ccaattgcat tattttgcat caggcggtga atgacctgat
     7081 ccgtgattat ttaggcatca aaggacccta tccccaaatt gaactcgttg cgggtgatga
     7141 tcagaccatc atcagtaaaa cgccgtatat catggattat ttcaaaggaa gcaagcttag
     7201 cgatgatttc tttgatgaag tggttttgta tccggatcaa ttggtatacc tgaacggcga
     7261 aatatccacc ggccggatgg accggaaggt gaatatccat cttcaaagcg ggactgttac
     7321 atataaaacc ggtttggcgg aggcccaaac gattgaagag actctgcttg cctacttata
     7381 tgtaatcaag ggcattcgta gccacggctt gcaaatcaag accatgacct cccaggaaat
     7441 tgggtttatc aagaactggg agagtgaggc ctatcggaaa aggcttacga aagaggcgac
     7501 ggccaaataa ggatagcgac taaaccccgc actgcgcgat ggcggcaggg caaaaaatcc
     7561 ggctgaagga aatggaggat acgggatgtc ccaatatatt ctggagttgc aaggcattac
     7621 caaaacattt cccggagtca cagccctgag cgacgttcat tttcaattac gccggggaga
     7681 gattcatgcc ttgatgggag agaacggcgc cggcaaatcc accttcatca aaataattac
     7741 cggagtgcat cagccggatg ggggagagat ttatttaaac gggaaaaaaa ccgtgatcaa
     7801 aagcccaaat gatgcccaaa gcctggggat cgcggcgatt tatcaacatg tcacctgtta
     7861 tccggatctg actgtaactg aaaatatttt catgggtcac gaacaagtcc agagattcac
     7921 cggaagaatt ttgtggcagc agatgcacag ggaagcaaaa acgctcctca ccgggctggg
     7981 agtggaaatt gatccaagga cccggatggg tgccttgagc gtggcccagc agcagaccgt
     8041 ggaaattgcc aaggccttgt cgatgcgtgc caagatcatt atcatggatg agccgacctc
     8101 ggccctaacc aagcgtgaaa gcgaggagct gtaccggatc accgaacaac ttagggataa
     8161 cggggcttcc atcattttca tctcccatcg catggaggat atgtacagat tagcggaccg
     8221 ggtcacggta ttacgggacg gacagtatat cggaacctgg gaggccgaag ggatctccgg
     8281 tgatca
//
LOCUS       DASVMB010000174         7849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_390207,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000174 DASVMB010000000
VERSION     DASVMB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390207"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VF531_05190"
     CDS             <1..978
                     /locus_tag="VF531_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713395.1"
                     /translation="YGGSEVIHGHLTTGSLVAFFAYILMAISPLTGITHNLNSIQQAL
                     AAADRVFETLDYEADIQDAPNAVAIPKIKGKIEFRDVRFSYQNNVPVLEHIQLTVNPG
                     EIVALVGPSGAGKTSLVNLIPRFYDPTEGQVLVDDHDIRSVQMKSLREQIGLVPQESI
                     LFGVSIRENIGYGKPGATEEEIVAAAKAANAHDFILNLSEGYATKVGERGASLSGGQR
                     QRIAIARALLRNPRILILDEATSALDTESERLVQEALERLMRDRTTFVIAHRLSTVKF
                     AHKIIVLAEGRIVEVGTHEELLAMGGLYQRLYEAGSVQSETDGSLKSNA"
     gene            971..2191
                     /locus_tag="VF531_05195"
     CDS             971..2191
                     /locus_tag="VF531_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase-related protein"
                     /protein_id="HEX7713396.1"
                     /translation="MPERNSSILFVSNGHGEDLIAARIIKSLIEQEPSLRLQALPIVG
                     RGAAYREMGIPLSLEGKTMPSGGFIRNGPANLWMDLKAGLWGLTGSQIKALRSVRERV
                     DLAVCVGDVVLLILAGCFLRRPILFLPTAKSDYVAPHWAVERFLMKRFCRKILTRDAK
                     TAASLSRYGLPAEFLGNVMMDALDYQGQDLKGSPGEWVIAVLPGSRLEAYDNLEDIAR
                     VALAFQPLVPAEKKVRYLVALSGGLALTELANRLEVMGWRGVNPEPEERLRGVVGHLE
                     YKQPPIRMTVLQGRFADILAACDGVLGMAGTANEQAVGLGKPVVTFVGRGPQFTAKFL
                     RTQKRLLGDSISVTDREPSASALELARILNDPARREAMARIGRERMGPTGGAARIAAA
                     IRSQTRDIILETAK"
     gene            complement(2235..3164)
                     /locus_tag="VF531_05200"
     CDS             complement(2235..3164)
                     /locus_tag="VF531_05200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7713397.1"
                     /translation="MLLASVVIPTHNQQHILEYCLQILFNQTASPDTYELVIVDDGSN
                     DGTSEMVQKLQEKAPCPLIYNWHPNIGRSLNRNTGALMAHGKYLIFLDGDMLVRREFV
                     SAHLSAHTKPGLIAHGPVVYTEELKDPDNGQKKVYDFSRAFFATGNVSLEREQFIASG
                     MFDADFIEYGWEDLELGERLRKMGLQTVRCPEAWSYHLQKRLTYEGIPGLIAKEKERG
                     HMGVLFYRKNPSIAVQLMTLISPIYFAYDRILTICHWPEKPGTLKLLQHLDRPGTRFL
                     FRLLVEIVKSHAYADGLREALAREGKSGNSNPR"
     gene            3389..4327
                     /locus_tag="VF531_05205"
     CDS             3389..4327
                     /locus_tag="VF531_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7713398.1"
                     /translation="MPSIFITGAGGCVGHYLVDQLTPEYELYLLVRNPAKLRFDPEQR
                     KNITVLKGDLDQIVDFAGVLSRVDYCIHAATAWGGEGTERINVRRVHELFGMLNPERI
                     RRVIYFSTASILGRDMRPLPEAEEYGTEYVRTKYRCYMGLPQIPCYERIVTVFPTLIF
                     GGDATHPFSNLSLSLPALRRYSGLLGRINIPLRFHFIHAQDIAKVVRYLLEEPTVAGN
                     YVLGNKPISLGDFTRRAAQYFGHPVRWQIRFTPALLYRLALICGARMSDWDRFCVRYG
                     NFEYPATDCASLGLASNYKELEQILADWRLVEASRT"
     gene            4704..5864
                     /locus_tag="VF531_05210"
     CDS             4704..5864
                     /locus_tag="VF531_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7713399.1"
                     /translation="MNYFLTEEQQMIKDLARRIADEKIAPKAQEWDETGEFPWEVMKV
                     LAESDLFGVYLPEEYGGLGGGVFEQCLVVEELSRACSGVAVSYAASGLGASPILVLGS
                     EEQKKKYLPQIASGKKLAAFAATEANAGSDPSGMQTTAVLDGDSYVLNGTKQWITNGG
                     EAEIYTVMAVTDRKKGPRGISCFIVEKGTPGFSFGKKENKMGIRASATRELVFDNCRV
                     PKENLLGGKEGVGFIATMKTFDRTRPGIGAQAIGIAQGALDAAVKYAREREQFGQTIS
                     SFQAIQHMLADMATQLEAARALVYATARMIDAGVKNISKESAMAKLFASDTAMKITVD
                     AVQVFGGYGYMKEYPVEKMMRDAKITQIYEGTNQIQRNIIALELIKEFASKK"
     gene            6087..6872
                     /locus_tag="VF531_05215"
     CDS             6087..6872
                     /locus_tag="VF531_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7713400.1"
                     /translation="MKIVVCVKQVPDTTEVKINPETNTLVREGVASILNPFDMYAVEE
                     ALRIKEKLGGSVTVISMGPPQVAEQLRETIGMGVDDAVLLSDRAFAGSDTLATSYALS
                     KGIAKIGAVDLIICGKQAIDGDTAQVGPEIAENLGIPHITYVKKVEEISAEGLTVERM
                     NEDGYDRVQLPLPALLTVVKEINEPRLPSLKGMMRAKKAVITTWSAADIGADPVRCGL
                     NGSPTWVKRIFIPEIKCQGEMFSGTPEAQVKSLVDALLTRRIV"
     gene            6916..>7849
                     /locus_tag="VF531_05220"
     CDS             6916..>7849
                     /locus_tag="VF531_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEX7713401.1"
                     /translation="MGIRVLIDKCYGCKVCVASCPFGIIHVVDKKAVIKEGCNLCGSC
                     VSACKFKAIEITRETTSTIDKTRYKGVWTFAEQRGGKLTNVALELLGEGRKLADALGE
                     PLVAVLLGHSVEGEAKRLIAHGADQVFLADAPELERFLEEPYTQVIVDLIRNQNPNIV
                     LMGATAIGRSLAPKIAARLGTGLTADCTGLDVDVAERNLMQTRPAFGGNLMATILCPN
                     HRPQMATVRPKVFKPMAADESRQGQVTRVDLSKTVWDLKAKILEVVNEVGHSVNLEEA
                     NIIVSGGRGLCDPKNFQLVQDLAAVLGGAVGASRA"
BASE COUNT         1881 a         1863 c         2205 g         1900 t
ORIGIN      
        1 tatggcggaa gcgaggttat ccatggacac ttaaccaccg gttctctggt ggctttcttt
       61 gcatacatct tgatggcgat atcgccgctt accggaatta cccataacct caactccatt
      121 cagcaagcat tggctgcggc ggatcgggtc tttgagacgc tcgattacga ggctgatata
      181 caagacgctc ctaatgcggt ggccatacca aagatcaagg ggaaaatcga gtttcgcgat
      241 gtccgcttta gttatcaaaa taacgttccg gtcctggaac atattcagct gacggtaaat
      301 cccggcgaga ttgtagcttt ggtcggtccc agcggtgccg gcaaaaccag tcttgtcaat
      361 ctgatacccc gcttttacga tcctactgag ggtcaagttt tggtggacga tcatgatatc
      421 cggagcgttc aaatgaaaag tttacgggaa cagatcggac ttgtcccgca ggagtcgatc
      481 ttgtttggcg tcagcatccg ggagaacatc ggttacggta aacccggggc taccgaagag
      541 gaaatcgttg ccgctgccaa ggcggctaat gctcacgact tcattctgaa tctgtcggaa
      601 ggttacgcta ccaaggtggg cgagcgcggc gcctcgcttt ccggcggcca acggcaacgg
      661 atcgccattg cccgcgcgct tttacgtaac ccccggatcc tgattcttga tgaagcgact
      721 tctgccctgg acaccgagtc ggaacgtttg gtccaagagg cactcgagcg gttgatgcgg
      781 gatcggacga cattcgtcat tgctcaccgc ctttccaccg taaaattcgc ccataagatc
      841 attgtcctgg ctgaaggccg gatcgtggag gtggggactc atgaggaact gctggcaatg
      901 ggaggtttgt atcagcggct ttacgaagcc ggaagtgttc aatccgaaac ggatgggagc
      961 ctgaaatcca atgcctgaac ggaattccag tattcttttt gtcagtaacg gacacggcga
     1021 ggatttgatt gccgctcgaa tcatcaaaag cctgattgaa caagaacctt ccttacgact
     1081 tcaggcatta ccgattgtcg gcagaggagc cgcataccgg gaaatgggca ttcctttgtc
     1141 acttgagggg aaaaccatgc ccagcggcgg ctttattcgt aatggtccgg caaacctatg
     1201 gatggatctt aaggccggtc tatggggtct aaccggatcc cagatcaagg ctttacggtc
     1261 tgttagggaa cgggttgatc tggctgtatg tgtgggagat gtggttctcc tgatcttggc
     1321 aggttgcttt ttgcgccggc cgattctatt tttacccacc gccaagtcgg attatgtggc
     1381 gccccattgg gcggtggaac gtttcctgat gaagcgtttt tgccgtaaga tcctaacccg
     1441 tgatgccaaa accgcagctt ccctgagtag gtatgggctg cctgcagaat ttttgggaaa
     1501 tgtaatgatg gatgccttag actatcaagg tcaagatctg aaaggttccc ccggagaatg
     1561 ggttatcgcc gtgcttccgg gcagtcgttt ggaagcctat gataatcttg aggatatcgc
     1621 ccgggtcgcc ttggcctttc aaccattggt acctgcggaa aagaaggtac ggtatctggt
     1681 ggcgctctcc ggaggattgg ccctcacaga actggcaaac cgcttggaag taatgggctg
     1741 gcgcggagtg aacccggagc cggaagaacg attgcggggg gtagtaggcc atctggaata
     1801 caaacaaccg ccaattcgta tgaccgtact acaagggcgc tttgcagata ttctggccgc
     1861 ctgcgatggg gttttgggaa tggccggtac cgcaaatgaa caggccgtcg gtcttggaaa
     1921 accggtggtc acttttgtag gccgcggccc gcaatttacc gccaagtttc tccggactca
     1981 aaaaagatta ttaggagatt ccatcagtgt gacggatcgg gaaccgtcag catccgccct
     2041 ggaattggcg cggattttga atgatcctgc ccgccgtgag gcgatggcgc ggatcgggcg
     2101 ggaacgaatg ggcccgactg ggggggcggc acggatcgct gcggcgattc gcagccaaac
     2161 acgggatatt attcttgaaa cagctaaata aagcgccgtt acggcgtttt ccgggatcgc
     2221 cggaggaaat tccttcaccg gggattagaa tttccggatt taccttcccg ggctaaggct
     2281 tcgcgaagtc catcggcata tgcgtggctt ttaacgatct ccaccagtag gcgaaagaga
     2341 aatcgtgtgc cggggcggtc gagatgttgc agtagtttca aagtgccggg tttctccggc
     2401 cagtggcaga tagtaagaat ccggtcgtag gcaaagtaga ttggcgagat taaggtcatc
     2461 aattgaacgg cgatagaagg atttttgcga tagaataata ctcccatgtg accccgttcc
     2521 ttctccttgg cgataagacc cgggattcct tcataggtca accgcttttg gagatgatag
     2581 ctccaggctt cggggcatct caccgtttgc agacccattt tacgcaaccg ttcaccaagt
     2641 tccaaatctt cccaaccgta ttcgatgaaa tcggcatcga acattcccga agcaataaat
     2701 tgctcccgtt cgagcgagac attaccggta gcgaagaagg cgcgggagaa atcgtaaacc
     2761 tttttctgcc cgttatcagg gtctttcagt tcttctgtgt agaccacggg accgtgggcg
     2821 atcaatcccg gttttgtatg ggctgacagg tgggcggata caaattccct gcgcaccaac
     2881 atgtccccgt ccaaaaagat cagatatttt ccatgggcca ttaaggcccc ggtatttcgg
     2941 ttcaaagagc gtccaatgtt gggatgccag ttgtaaatca agggacaggg cgctttttcc
     3001 tgaagttttt gtaccatttc cgaagtgccg tcgtttgaac cgtcatctac gatgacgagt
     3061 tcataagtat cgggcgatgc ggtctggtta aacagaattt gcaggcaata ttcgagaatg
     3121 tgttgttgat tgtgagtggg aatgaccaca ctggctaata acaaggcaat actcctttat
     3181 gatacttttt aagacatatc cattaacaaa tcgccattat tttagcacta aagtgattta
     3241 ggtgtcaatg ttcgtaggcg gatttaaggt tttgtgggtt tattggacct aatcggattg
     3301 tatcgggata ggaaaatcac aaagcgccgg cgaattttaa acgtaaagca ttgagcctga
     3361 ttaactgaaa aagcggagga gcgatttaat gccatccatc ttcatcaccg gggccggcgg
     3421 ctgcgtcgga cattacctgg tcgatcagtt gacccctgag tacgaactct acctcttggt
     3481 gcgtaacccc gccaaacttc gctttgatcc ggaacagcga aagaatatta cggtcttgaa
     3541 aggcgatctg gatcaaatcg tcgattttgc cggtgtcctt tcgcgggtgg actattgtat
     3601 tcacgcggct acggcctggg gaggggaagg aaccgaacgg atcaatgtcc ggcgtgtaca
     3661 tgaactcttc ggaatgctaa acccggagcg tatccggcgg gtgatatact tttccactgc
     3721 cagcattctg gggcgggata tgagacccct gccggaggcg gaagaatacg gaaccgaata
     3781 tgttcgcaca aaataccgct gttacatggg tttacctcaa atcccttgtt acgagcggat
     3841 cgttacggtt ttcccgacgc tcatcttcgg cggcgacgcc actcatccct tttccaacct
     3901 tagtttgagc ctgccggctt tacggcgtta ttcgggactg ttgggacgga tcaatatccc
     3961 gttgcgattc cattttattc atgcccagga catcgcgaag gtcgtacgct atttgcttga
     4021 agaaccaacg gtagcgggga attacgtttt ggggaacaaa ccgatctccc tgggcgactt
     4081 tacccgacgc gccgctcagt acttcggaca tccggtccga tggcagatta ggtttactcc
     4141 ggcgctcctt tatcgcctgg cgttaatctg cggcgcccgc atgtcggatt gggaccggtt
     4201 ctgtgtgcgg tacggtaatt ttgaatatcc ggcaaccgac tgcgccagtc tcggattggc
     4261 cagtaattat aaggagctgg aacagattct ggcggattgg cgattggttg aagcatcgcg
     4321 cacctgactt tgaccggtgc catagaaatt ggcggcgtgt gtatggcgaa atagggtatg
     4381 atatagtgta gagtattaac tgcaacgata aaggacggag aaactcccgt tcgaccttaa
     4441 cttgacaagc taaaacggct tatgttagac tgatatatat ttatgcatcg gccggtccct
     4501 ttggtgtccg agatggtttt gcgcctacca gccgcaattg atccggaact ccgacttact
     4561 tgtctgccat gtatgatttg gcaagataat gcgttttcct taaactctat gccggtttga
     4621 gactacacta catagattga tgggccaatt aatttgtatt ttaaccaaaa cgggatgaag
     4681 acaattattg gagggtgatg gctgtgaact attttttgac tgaagaacaa cagatgatta
     4741 aggatttagc gcggcggatc gcggatgaaa agatcgctcc caaagcccaa gaatgggatg
     4801 aaaccgggga atttccctgg gaggttatga aggtactcgc cgagtcggat ctcttcggcg
     4861 tatatttgcc tgaggaatac ggcggcttag gcggcggcgt ttttgaacaa tgcctggtgg
     4921 ttgaggagtt gtcccgcgcc tgttccggtg tggcggttag ctatgccgct tcgggtttag
     4981 gcgcttcccc gatcctggtg ttgggtagcg aggaacaaaa gaagaaatac ttgcctcaga
     5041 tcgcatccgg caagaaactg gcagcttttg cggcaacaga ggccaatgcg ggcagcgacc
     5101 cgtcggggat gcaaactacc gcagtgcttg atggagacag ctatgtccta aacggcacga
     5161 aacaatggat caccaatggc ggcgaggcgg agatttacac cgtgatggcg gtaaccgata
     5221 ggaagaaggg cccccggggg atcagctgtt ttatcgtaga aaaggggact cccggtttca
     5281 gttttggaaa gaaggaaaat aaaatgggga tccgggcttc ggctacccgg gaattggtct
     5341 ttgacaactg ccgggttccc aaagaaaacc tgctcggcgg aaaagagggc gtcggtttta
     5401 tcgcaaccat gaagactttt gatcgtaccc gtccgggaat cggggcccag gccatcggta
     5461 tcgctcaggg tgcactggac gcggcggtca aatacgcacg tgaacgggaa caatttggac
     5521 aaaccatctc ctcattccag gcgatccagc atatgctggc agatatggct actcagctgg
     5581 aggcagcccg ggctttggta tacgccaccg cccggatgat cgatgccgga gtcaagaata
     5641 tctccaagga atcggccatg gccaagctgt tcgcatcgga tactgccatg aagatcaccg
     5701 tagatgcggt ccaagtcttc ggcggttacg gttatatgaa ggaatacccg gtggagaaga
     5761 tgatgcgtga tgccaagatc actcaaatct atgaaggcac caaccagatc cagcgaaaca
     5821 tcatcgcact tgagctgatc aaggagttcg cctccaaaaa gtaagtttga tggtttatac
     5881 aacgaaacac accaaagaca cgaaaatgga tatgactttt ttgcggccat tgaagtaatt
     5941 tatctatcta tgcaataaag gattttagat caacaatttt tgaaacagaa ttcaaattta
     6001 aaaacgttcc ggcgccctgt agggcgcttt ttatggtaag ctgcactaag cgcccgcgct
     6061 tagtggagat ataaggggga gatactgtga agatcgtagt ttgcgttaag caggttccgg
     6121 ataccaccga agtgaagatc aacccggaaa cgaacacatt ggtccgggag ggtgtggcca
     6181 gtatcctgaa tccctttgat atgtatgcgg tggaggaggc tctgcgtatc aaggagaaac
     6241 tggggggaag cgtcaccgta atctccatgg gcccgcccca agtggcggaa cagctccggg
     6301 agaccatcgg aatgggagtt gacgatgcgg tcctcttatc cgatcgtgcc tttgccggtt
     6361 ccgacacctt agccacttca tatgccctgt ccaaggggat cgccaagatc ggcgcagttg
     6421 atctgatcat ctgcggcaaa caggcgattg acggcgatac cgcccaggtt ggcccggaga
     6481 tcgctgaaaa tctcgggatc cctcatatta cctatgtcaa gaaggttgag gagatttctg
     6541 cggaaggatt aaccgtggaa cggatgaacg aggatggtta cgaccgggtt caactgccgt
     6601 taccggctct gttgactgtg gtcaaggaga tcaacgagcc acgtcttcct tccctcaaag
     6661 gaatgatgcg cgccaaaaaa gccgtcatca ctacctggtc agctgctgat atcggtgctg
     6721 atcccgttcg ctgcggtttg aacggctcac cgacctgggt aaaacggatc ttcattccgg
     6781 agatcaaatg ccaaggggag atgttcagcg gtacccccga agcacaggtt aaatcgttgg
     6841 tcgatgcatt gcttacccgg aggattgtct gaaataaaca ttcttggttg ggccagtgaa
     6901 tgaaggagga tcaggatggg gattagagtt ttgattgata aatgttatgg ctgcaaggtt
     6961 tgcgtagcca gttgcccttt tggtatcatt catgtcgtgg ataagaaagc tgtaatcaag
     7021 gaaggctgca atctttgcgg ctcttgcgtc agtgcttgta aattcaaggc gatcgaaatc
     7081 acccgggaga ccacaagcac gattgataaa acacgttaca aaggggtttg gacttttgcg
     7141 gaacaacgcg gcggcaagtt gaccaacgtc gctctggagc ttttgggtga aggacggaaa
     7201 ctggccgatg ccttagggga accgttggtt gccgtcttac tcggccacag cgtcgagggt
     7261 gaagccaaga ggctcatcgc ccatggagcg gatcaggttt ttctggcgga tgctccggaa
     7321 ttggagcgtt ttctggaaga accctatact caggttatcg tggatctaat caggaaccaa
     7381 aatcccaata tcgttttaat gggcgccacc gccattggaa gatcgctcgc tccgaagatc
     7441 gctgcccgac tgggcaccgg tttgactgcc gactgtaccg gtcttgatgt ggatgtggcc
     7501 gaaaggaacc tcatgcagac cagaccggct tttggaggca acttgatggc tacgatcctt
     7561 tgccccaacc accgccccca aatggctacg gtacggccga aagtctttaa gccgatggcc
     7621 gccgacgagt cgcgccaggg tcaagtcacc agggttgatc tctcaaaaac cgtttgggat
     7681 ttaaaagcta agattttaga ggtcgtcaat gaagtggggc attcggtcaa tcttgaggag
     7741 gccaacatca tcgtctccgg tggccggggt ctctgcgacc ccaagaattt tcaactggtt
     7801 caggatctcg ccgctgttct agggggagcg gtaggcgcct cccgcgccg
//
LOCUS       DASVMB010000175         6292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_316531,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000175 DASVMB010000000
VERSION     DASVMB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316531"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF531_05225"
     CDS             <1..214
                     /locus_tag="VF531_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713402.1"
                     /translation="GFSFERGYNMMNPLVMSVADNLSIFVYNLGLRALRFNVATAVGL
                     FQSVVGFILVMITNYAANKAGEQGIT"
     gene            280..1152
                     /locus_tag="VF531_05230"
     CDS             280..1152
                     /locus_tag="VF531_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014371307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713403.1"
                     /translation="MKSDFKSKIFNFIVYFTLGLTVLICILPFLHIMAISLSDNSAVM
                     SQKVSLFPIGFNIKAYIEVFSDQTMMDSLGFTALMTLLVAVIGLLITIALAYPLSRKS
                     LKGRKIFMYLILFTFYFNAGIIPNYINIQELGLFNSIWVLILPVVLSPFNMILMKNYI
                     QTAIPESLEESAKLDGCSEVGFLIRIVLPLSKPILATIALFFAVGRWNGFQDALYYIQ
                     SKPLYPLQLKLFYLVSAAQSTESFSAEGMATAFTPEVVRAASIMFATIPILIVYPFLQ
                     KYFVKGALLGSIKE"
     gene            1226..2812
                     /locus_tag="VF531_05235"
     CDS             1226..2812
                     /locus_tag="VF531_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713404.1"
                     /translation="MNRKLSISVCLALVLIMLVSSTVTFASKKAPVEIRIPVFERGRQ
                     GQSPADTGYWAKYIQDKVLKDLNIKLTWVPIARPNPAGTKEAFNLLIASNTAPDIITE
                     YDISPGYMGWYGQGVFQEVPVSLVKKYAPNYYKFVGPDIINIGKIKGKLIFLPAKRPL
                     WMGSTWVNYIRQDWLDKLGLRNPRTLDEYYEVVKAFKTKDPGGVGPERVIGIPLDLNG
                     ATLANINYLWRPDKISDREKYLYSDVALPSLPWEPTRQALKWHNKAYNDGLINPEFML
                     DTNGEKMKAAFLNGYTGFWGEYITTDAGYIDTLMKNCPTAKLSVRYPYRRDEKVAPPY
                     YVDTPPVGLMSGINRNCKHVPEVLKYLDWLSRPENLNVLQWGTEGKTYKEVNGVRQLL
                     AYSGDERLLNGNNKDYYCLVAEGVDFGDDSKNLANAACPAGDQYRYLLQDSYKYSKMP
                     YQKPSPNVFIPAVISAQSSLGPSLVTKYREYACRLIICKPSEFDALYEQYKAEYLAAG
                     YQKILDEKAKIYDEWFGKKK"
     gene            3067..3795
                     /locus_tag="VF531_05240"
     CDS             3067..3795
                     /locus_tag="VF531_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEX7713405.1"
                     /translation="MNNRSDSSEVKIKSRQGIKIFVAGDSTAADKQPDKRPETGWGQV
                     IGRFFNEDVQIRNYAINGRSSKSFVDEGNLKEIAAEIGENDYLFIQFGHNDEKDVVKL
                     HTDPQRTFKQCLAEYVTCARIAKAFPVLLTPVQRRFFNEQGGIVNTHGQYPAAMRELA
                     REMQVPLLDLSEVSKNFFESLGDEESKRIFLWLKPGESPNYPEGVQDNTHFCEYGAIE
                     IARLIIEEIRKVDGLKDLTLRFKK"
     gene            complement(3864..4142)
                     /locus_tag="VF531_05245"
     CDS             complement(3864..4142)
                     /locus_tag="VF531_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7713406.1"
                     /translation="MTKADLIDKVAKDTKLTKKDSDQAIDAIVKAISGALAKGDKVTL
                     VGFGSFEVRKRAARQGRNPQTGKAINIPARKVPVFKAGKALKDAVAKK"
     gene            4745..>6292
                     /locus_tag="VF531_05250"
     CDS             4745..>6292
                     /locus_tag="VF531_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7713407.1"
                     /translation="MEQRRWLKAYEKGIPAQIEYPSLTLSEVLHNSAVRFGSQTSMIY
                     LEAQWTYQELDDLVSRMANLLIEVGIQKGDRVGVQLLNSPQFVVAYFGILRAGAIAVP
                     INPHFTGDDLGYIIKNSGMKLIVTGVENIPVLEQVRTKDLRVILTDIKVPFKENHQYK
                     APVDAVVLEAELFKHSAVDPQVNITPDAIANLQYTGGTTGINKGAILTHRNLVVNATQ
                     FRVWFKNVYRDGEGKFVCVIPMYHIYAMSTTMNHAILSGSSILILSKFDLNELMRLID
                     KYKPNLFMGVPAMYGAIAMREGHNYDLSSIEACMCGSAPIPVAIHEKFEKITGGKLRE
                     GYGLSECSPAVSLTPIYGRVKHGSTGVPVPDTDVKIVDPQTGLEITKPGVVGEIVVKG
                     PQVMQGYWEQPEETELVMKDGWLHTGDLASMDEDGYVFIADRLKDMIITGGEKVYPRE
                     IEDLLYTHPAVKEVAAIGVPHPLRGEVPRAYVVLKEAAATSERELRQYCAEHLSKFKV
                     PHQIEIIG"
BASE COUNT         1794 a         1276 c         1505 g         1717 t
ORIGIN      
        1 cggtttttcc tttgaacgcg gctataatat gatgaatcct ctggtaatga gtgtcgccga
       61 taatctaagt atctttgtat acaatctggg attgagagcg ctccggttca acgttgccac
      121 agcagtaggc ctgttccagt ccgttgtcgg attcatactt gtcatgataa ccaattatgc
      181 cgccaataaa gccggtgaac agggcatcac ttgataagaa agatagaatc ctgcaatccg
      241 tgaaccggaa ttcaccaatg acaaacagga ggattatcaa tgaaatcgga ttttaaaagc
      301 aagattttta actttattgt ttattttaca ttagggctaa ccgtattaat ctgtattctt
      361 ccgtttttgc atatcatggc aatttccttg agtgataact ccgcggtgat gtcccaaaaa
      421 gtctcgcttt ttccgattgg ttttaatatc aaggcatata ttgaggtgtt ttccgatcaa
      481 accatgatgg attcacttgg atttacggcg ctcatgactt tattagtagc cgtcataggc
      541 ctgttaatta caatagcgct ggcttatccg ttatccagaa aaagtctcaa gggaagaaaa
      601 atatttatgt atctgatatt gttcaccttt tatttcaacg ccggcatcat acccaattat
      661 attaacatcc aggaacttgg ccttttcaat tcgatatggg ttttgatact tccggtagtg
      721 cttagtccct ttaatatgat acttatgaaa aactatattc aaaccgccat tcccgaaagt
      781 ctggaagagt cggccaaact tgacggttgt tctgaagttg gttttttgat aaggatcgtg
      841 ctgccattat caaagccgat actggctacg atcgcattat tcttcgcggt cggaaggtgg
      901 aatggattcc aggacgccct ttactatatt caatccaagc ctttgtatcc cttgcagcta
      961 aaactctttt accttgtaag cgccgcgcag tccacagagt cattctcggc cgaaggaatg
     1021 gcgaccgctt ttactcctga agttgtaaga gcggcatcaa taatgtttgc tacaatcccg
     1081 atcctgattg tctatccctt cctgcagaaa tacttcgtca agggcgcatt attaggctct
     1141 atcaaagaat gacggtagtt tttgccttac ttcgttaata acaacctcgg gttgttgtta
     1201 aaataaaaac gaggagggag tttttatgaa ccgtaagctt tccatttcag tgtgtcttgc
     1261 tcttgtgctg atcatgctgg tgtcatccac ggtgaccttt gcaagcaaga aagccccagt
     1321 ggaaatcaga atcccggttt ttgaaagggg aagacagggc cagtcgcctg ccgataccgg
     1381 ttattgggca aagtatatcc aggataaggt tttaaaagac cttaacatca agctcacctg
     1441 ggtgcccatc gccagaccca atccggccgg aaccaaagag gcgtttaatt tgcttatcgc
     1501 ttccaacaca gctcccgata tcatcaccga atatgatata tcgccggggt atatgggatg
     1561 gtatggtcag ggagtatttc aagaggttcc ggtaagcctt gtaaagaaat atgcgcccaa
     1621 ttattataaa tttgtaggcc ccgacatcat caatatcggt aaaatcaaag gcaagctaat
     1681 cttcctccct gcaaaaagac cgttatggat gggatcaacc tgggttaact atatcaggca
     1741 ggactggctt gacaagctgg gattgcgcaa tccgaggact ctggatgaat attacgaggt
     1801 ggttaaggct ttcaagacca aggatccggg cggagtcggc ccggagagag taatcgggat
     1861 accattggat ctaaacggcg caactctggc taatattaat tacctgtggc ggccggacaa
     1921 aattagcgat agggaaaagt atttatactc cgatgtggct ttgccgtcat taccctggga
     1981 acctaccagg caagcattaa agtggcataa caaggcttat aacgacggac tgatcaaccc
     2041 cgaattcatg ctcgatacca acggcgaaaa gatgaaggcg gcatttttaa acggctacac
     2101 cggtttctgg ggtgaatata tcaccacgga tgcgggttat atcgacactc tgatgaagaa
     2161 ctgcccgaca gctaaacttt ccgtacgtta tccgtaccgg agggatgaaa aggtagcccc
     2221 gccctactat gtggacactc ctccggtagg tttgatgagc ggtatcaaca ggaactgcaa
     2281 gcatgtgccg gaagttttaa aatacctgga ttggctctcc agaccggaaa acctcaatgt
     2341 tcttcagtgg ggcactgaag ggaaaacata taaagaagta aacggagtca ggcagctgtt
     2401 ggcatacagt ggagatgaac ggcttctcaa cgggaacaac aaggactatt attgtctggt
     2461 ggccgagggc gtagatttcg gggacgatag taaaaacctg gccaatgccg cttgcccggc
     2521 gggagatcaa taccgatatc tgttgcagga cagctataaa tattcaaaaa tgccgtacca
     2581 gaaaccttct cccaatgtct tcatccctgc ggttatttcc gcacagtctt cattaggacc
     2641 gtcactggtt acaaaatacc gggaatacgc ctgcaggctg ataatctgca agccttctga
     2701 atttgacgca ctatatgaac aatataaggc ggaatatctt gcggccggat atcaaaaaat
     2761 actggatgaa aaagcaaaaa tctacgacga gtggtttggc aagaagaaat aatgtagtct
     2821 aaagactttt atatttattg gacctttatt tttgaggggg gtgaaattac gacccccctc
     2881 aaaatttata tttatagaat ttgatgaaaa tagacaaagg ggatactgaa tgcatcgcag
     2941 aattcaagag gttagacagg caaaccccaa tggtgaagcc cgaattatat tgaaccgata
     3001 aaaccgcggg agatgatgga aatataaccc tgcaaattga tgaaagaatt ttaaggagga
     3061 actgagatga ataacagatc tgatagcagc gaggttaaga tcaaatcccg gcaaggaatt
     3121 aaaatttttg ttgccggtga ttccaccgca gccgacaagc agccggacaa acgtcctgaa
     3181 accggatggg gacaagtaat tggccgcttt tttaatgaag atgttcaaat tcggaattac
     3241 gcaataaacg gaagaagctc gaaaagcttt gtcgacgaag gcaatttaaa agaaatcgca
     3301 gccgaaatcg gggaaaatga ttatttattt atacaatttg gccataatga tgaaaaagat
     3361 gtcgtgaaat tacatacaga tccgcaaaga acttttaagc agtgccttgc tgaatatgtg
     3421 acatgcgcga ggattgcaaa agcgtttccc gtactattaa ccccggtcca gagaagattc
     3481 tttaacgaac aggggggtat tgttaatacc catggacaat atcctgcggc aatgagagaa
     3541 ttggccaggg agatgcaggt tcccctgctg gacttaagtg aagtcagtaa aaattttttt
     3601 gaaagcttag gggatgaaga gtcaaaacgc atctttttat ggctgaagcc cggagaaagc
     3661 ccgaattatc ccgaaggggt acaggataat acccattttt gcgagtatgg ggctattgaa
     3721 attgcgcggt taatcattga ggagataaga aaggttgatg gtttgaaaga cttgactttg
     3781 aggtttaaaa agtaggactt ttttagaaag atacccgagg tgagataata aaaaaacccg
     3841 tccattcagg acgggttaat tttttacttc tttgctaccg catcttttaa ggcttttccg
     3901 gccttaaaca ccggtacttt ccgggcgggg atattgattg cttttccggt ttgaggattt
     3961 ctcccttggc gggccgcccg ttttctaacc tcgaaactgc cgaaaccaac cagggtaact
     4021 ttgtcaccct tggcaagcgc tccgctgatg gccttgacaa tggcatcaat ggcttggtcg
     4081 gagtctttct tggtgagctt ggtatctttc gctaccttgt caatgagatc tgctttggtc
     4141 aagataaatg cctcctttat tatttgggta atgaatgaag ctcaataata ctgcttggct
     4201 tctcttacca tataaatatt cgtcagatat ttaggtaatc tttatggata tttttaaagt
     4261 tgatcagatt tatcggtcaa tgaatggatt tccatccctt tactgtaaat tggtttacaa
     4321 ttagtttatg gatgctaatt aatcgggatt aagccagtgt gattcaatgc ggccatcaaa
     4381 atggatgccg ccgcgtgaga gcggtttgcg ccgttagaag tcaaaagact gtgggatgaa
     4441 ttatattcgg tcaatgcata atcccaaaaa aggggctgac tgaaaaagtc ggcccctttt
     4501 ttggtacgcc cggcatggat gttgactata atcgcgatta tacctatacc ggtataagta
     4561 tctcttccta aaatatcata ttttgaaaaa ggatgcaaac acaaattatg gaaaagccat
     4621 gtcaaccgga gtcgtatagg agagaatacg caggacggat catttttttt gcaggaaaat
     4681 aactattttt aacgaaatat tttgttcgtt tttttatgtc atgcaaccga aaggggaatc
     4741 tgttttggag caaagaagat ggctcaaagc atacgaaaaa gggattccag ctcagattga
     4801 gtatccatcc ctaactctat cagaggtcct gcacaattct gccgtcaggt ttggcagtca
     4861 gacttccatg atctatttgg aggcgcaatg gacttatcag gaacttgatg atctggtatc
     4921 caggatggcg aatcttttaa ttgaagttgg gattcaaaaa ggggatcggg tgggagttca
     4981 actcctcaat tcgccgcaat tcgtcgtagc ctattttgga attttgcgtg ccggggcaat
     5041 cgcggttccg atcaatcccc attttaccgg ggatgatctg ggatatatta ttaaaaacag
     5101 tggaatgaag ctgatagtaa ccggtgttga aaacataccg gtgctagaac aagtccgtac
     5161 caaagattta agggtaattt taactgatat caaggttccg tttaaggaaa atcatcaata
     5221 taaggcgccg gttgatgcgg tggtgcttga agcggagctt tttaagcatt ccgccgttga
     5281 tccccaagta aacattacac cggatgcgat cgccaattta cagtataccg gtgggacaac
     5341 cgggattaat aaaggcgcca tacttaccca tcgcaacctg gttgtaaacg ccacccaatt
     5401 tcgtgtttgg ttcaaaaatg tatatcggga cggggaaggt aaatttgtct gtgtgattcc
     5461 gatgtatcat atttacgcca tgagtaccac gatgaaccat gccatactta gcggttcctc
     5521 aattttgata ctctcgaagt ttgatctcaa tgaattaatg cgcttaatcg ataaatataa
     5581 acccaactta ttcatgggtg tcccggcgat gtacggcgcg attgcaatgc gtgagggaca
     5641 taattatgat ttgagttcca tcgaagcctg tatgtgcggt tccgctccga tcccggttgc
     5701 gattcatgag aaatttgaaa aaataaccgg aggcaagttg cgggagggtt atggactttc
     5761 cgaatgctcc ccggcagtgt cgctgactcc gatttacgga cgggtgaaac atggcagcac
     5821 cggagtcccg gttccggata cagatgttaa aatcgttgat ccgcagaccg gtttggagat
     5881 taccaagccg ggtgtggtgg gggagattgt ggtaaaagga ccacaggtca tgcagggata
     5941 ctgggaacaa cccgaggaaa ctgaattggt gatgaaggac ggttggctgc ataccgggga
     6001 tttggccagt atggacgaag acggttatgt tttcattgcc gaccgcttga aggatatgat
     6061 tattacgggg ggcgaaaaag tatatccgcg ggagattgag gatctgctat acactcatcc
     6121 tgccgtcaaa gaggtggccg ctatcggcgt tccccatccg ttgcggggtg aagtgccccg
     6181 agcttatgtg gtcttaaagg aggccgcagc aacctccgaa agagagttgc gtcaatattg
     6241 cgccgaacac ctttcaaagt ttaaggtgcc tcatcaaatt gagatcatcg gt
//
LOCUS       DASVMB010000176         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_465330,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000176 DASVMB010000000
VERSION     DASVMB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465330"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..842)
                     /locus_tag="VF531_05255"
     CDS             complement(9..842)
                     /locus_tag="VF531_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HEX7713408.1"
                     /translation="MLELPEAAVVSEQISRTLKGKRIMNVNTNQSPHKFAFFNGDPQN
                     YHGLLTEKKIEKAVSYGGMVEIAAGDARIILGDGVNLRYYATGEKLSEKHQLQLEFDD
                     FSSLIGSVQMYGFLWAFPAGQFDNPYYLVAKQKKSPLTEEFNDTYFSSILKDEKLNLK
                     SLSAKAFLATEQRIPGLGNGVLQDILWTARIHPKRKMITLSDAELDNLFKAVKSVLSE
                     MTLKGGRDTERDLYGCSGGYQTILSKNTAAKPCPVCGTEIKKEAYLGGSIYYCGGCQR
                     L"
     gene            complement(857..1264)
                     /locus_tag="VF531_05260"
     CDS             complement(857..1264)
                     /locus_tag="VF531_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HEX7713409.1"
                     /translation="MELLAKCGFRCDLCPAYKENIKTDADRVRVSEGWRRIFGFEFTP
                     ENVVCVGCHNEGNHADANCPVRQCTMEKDLPNCAGCDGFGCEALKTRTDFLEDYISKL
                     PDISEEDYQTYLLAYESKTRLMKLRNRKTESQG"
     gene            complement(1277..1879)
                     /locus_tag="VF531_05265"
     CDS             complement(1277..1879)
                     /locus_tag="VF531_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713410.1"
                     /translation="MLTKETLVNRKEDLENLAKEIGEGELELLVEWLKEKDDNIRYAA
                     FLLLQSLSAADNRVYRYWETLARMIGDPNSYQRSLGLMLISENIKWDKENKFATICDE
                     YLSHCDDEKFITARQCIQGLHKVLEHSTMYRDQIVNALVKIDLGKRKDTQQGLMLLDI
                     IGVLEKIYDETAEMRVAEYFRAKYEHGDARVRKTLKKYIS"
     gene            complement(1929..2444)
                     /locus_tag="VF531_05270"
     CDS             complement(1929..2444)
                     /locus_tag="VF531_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEX7713411.1"
                     /translation="MLHIFSKNETVEPRIVVLDKPIAAVGLSIRTGMKSVFKDLPKVY
                     DRYMKLKAQNGIPNIKEPWEYVSLSKNFTDEKSWDYYTGHVVTSRNAVAEEFLGFEVP
                     AGTYAVFPIRGKGKLFFGMAVGKMKRYIYETWLPQSQYEFGGYEFEYNNEAMHAENPY
                     AIDLYVAINGK"
     gene            complement(2494..2769)
                     /locus_tag="VF531_05275"
     CDS             complement(2494..2769)
                     /locus_tag="VF531_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7713412.1"
                     /translation="MSEESIKICQSCAMPLNDPNLWGSNADGSKNEDYCLYCYNDGRF
                     TTEESMEEMIESCVPHTSHNNPWPDDATAHKAMHEIFPKLKRWQNRV"
     gene            complement(2859..3779)
                     /locus_tag="VF531_05280"
     CDS             complement(2859..3779)
                     /locus_tag="VF531_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017531386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX7713413.1"
                     /translation="MKIDRLVAITTILLNKGTTTAKELAERFEVSTRTIYRDIEDLSA
                     AGVPVYAHQGNGGGISIMENFSLDRAIVTDRESESILLALKTLQAVNYPEVDLVLDKL
                     GAIFKNAAAADWVEIDFSPWGSGSKEKLKFQTVRRGILERLVLEFEYLGANGKRSHRM
                     VEPMKLLFKARAWYLWAWCRSRSAFRTFRITRMRDVVLTDQNFTRRDDGLDIKNSEEN
                     LPDQRDWVDLRLRFKPGAAYRLYDDFDETRIKRNEDGSLEMNQQFPEDEWVYGYLLSF
                     GPDLEVLEPEYIRKLLCDRMRQALGVYEKG"
     gene            3941..>4298
                     /locus_tag="VF531_05285"
     CDS             3941..>4298
                     /locus_tag="VF531_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEX7713414.1"
                     /translation="MEKAIVRNGIDNIAKYSELFAGKRVGLITGPTGVNKQLKSTIDI
                     FAEMFNLTTLYAPEHGIRGERQAGATVETYQDERTGLTVYSLYGMNKKPSSEMLANVD
                     ILAIDIQDVGIRYYTYL"
BASE COUNT         1052 a         1149 c          898 g         1199 t
ORIGIN      
        1 catgacgttt ataacctctg acaccctccg caataataga tactcccacc caagtacgcc
       61 tcttttttga tctccgtccc gcagaccggg caaggtttgg cggcggtatt tttgctgagg
      121 atggtttgat acccgcccga acagccgtat aaatcccgct cggtgtccct gccgcccttg
      181 agcgtcatct cggagagcac cgacttcacc gccttaaaca gattgtccag ttccgcatcg
      241 gataaagtaa tcatcttccg tttcgggtgg atccgggcgg tccataaaat atcctgcaag
      301 accccgttgc ccagtccggg aatccgttgt tccgtggcga gaaatgcctt ggcagacagg
      361 gattttaagt tgagtttttc atctttcaag atggaagaga aataggtgtc gttaaattcc
      421 tcggtaagcg gcgacttttt ttgctttgcg accaaataat acggattatc aaattgccct
      481 gccggaaatg cccatagaaa gccgtacatt tgcaccgaac cgatgagtga ggaaaaatcg
      541 tcgaactcca gctgtaattg gtgcttttcg gatagctttt caccggtggc atagtaccgt
      601 aaattcaccc cgtcccccag aatgatccgg gcatcaccgg cggcaatctc caccatacct
      661 ccatatgaaa cggccttttc aatcttcttc tcggtaagca atccgtgata gttctgggga
      721 tcaccgttaa agaatgcgaa tttatgcggt gattggttgg tattaacatt catgatccgt
      781 ttgcctttga gtgttctgct gatttgttcg gagaccacag ccgcctcggg tagttccagc
      841 atgatccttc cccctcttat ccttgtgact cggtttttcg attccgcaac ttcatcaagc
      901 gcgttttact ctcatacgcc aaaagatacg tttggtaatc ctcttccgat atatccggaa
      961 gtttggagat ataatcctca agaaaatccg tcctggtttt cagcgcttca cagccaaacc
     1021 cgtcacagcc cgcgcaattc ggtagatctt tctccattgt gcactgtctt acgggacaat
     1081 tggcgtcggc gtggttgcct tcattgtgac aaccgacaca aacgacgttt tcgggagtaa
     1141 attcaaaacc gaagatccgc cgccatcctt cactcaccct gacccgatcc gcatcggttt
     1201 tgatgttttc cttataagcc gggcaaagat cgcaccggaa accgcacttt gcaagcaatt
     1261 ccatatccca ttccccctac gaaatatact tcttgagggt cttcctcacg cgggcatcac
     1321 catgctcata ttttgccctg aaatactctg cgacccgcat ttccgctgtc tcatcgtaga
     1381 tcttttccaa tactccaatg atatccagca acattaaacc ttgctgggtg tccttccgct
     1441 tccccagatc gatcttgacc agcgcgttca cgatctgatc ccgatacatc gtggaatgct
     1501 ctaacacctt gtgcaatccc tgaatacact gacgcgcggt gatgaatttc tcatcatcgc
     1561 aatggcttaa atattcgtca cagattgtgg caaacttgtt ttccttgtcc catttgatat
     1621 tttcggagat caacatcagt cccagacttc tttggtagga gttcggatct ccgatcatcc
     1681 gggccaatgt ctcccaataa cgatacactc tgttatctgc ggccgaaagc gactgcaaca
     1741 gtaaaaaagc ggcgtagcgg atattatcat ccttttcctt gagccattcg acgagtaatt
     1801 ccagctcacc ctcgccaatt tctttggcga gattttcaag atcttctttc cgattaacaa
     1861 gcgtttcctt ggttaacaac ccatctgctc ctttcatttc cggtgccatg catatcccgt
     1921 tgttcgactt atttcccgtt aattgcgaca tatagatcga tggcgtatgg gttttcggca
     1981 tgcatcgctt cattattgta ttcaaattca taaccgccga attcatattg cgactgggga
     2041 agccacgtct cataaatata ccttttcatt ttacccacag ccattccgaa gaacaatttg
     2101 cctttacccc tgatggggaa aaccgcatag gttcccgccg gtacttcaaa ccccaaaaac
     2161 tcttcagcaa ctgcattccg gctagtcacc acatgaccgg tgtaataatc ccaggacttt
     2221 tcgtcagtga agtttttgct gagcgaaacg tactcccagg gttctttgat attgggtatt
     2281 ccgttttggg ctttaagttt catataacga tcataaacct tcgggagatc cttaaaaacg
     2341 cttttcattc cggtccggat cgataaaccg acggctgcga taggtttatc gagtacaaca
     2401 atcctgggtt cgactgtttc attttttgaa aagatatgaa gcacgacata acctcctgta
     2461 tgatcttcgt aatttttcaa gcggcactat cacttatacc ctgttttgcc aacgcttcag
     2521 tttgggaaag atctcgtgca tcgccttatg cgccgtcgca tcatcgggcc acggattgtt
     2581 atgggatgtg tgcgggacac aagactcgat catctcctcc atggactctt ccgtggtaaa
     2641 acgaccgtcg ttataacaat acagacagta atcctcattt ttgctgccat cggcattact
     2701 cccccataaa ttgggatcat tgaggggcat tgcacagctt tgacagattt ttatggattc
     2761 ttcactcatt ccacttcctc ctttactttt tttgttaatc tgacttcatt ttacataatc
     2821 gtacttgaca gccgtatgtc atatttaaaa ttcccatatt atcctttttc ataaaccccc
     2881 agggcctgtc gcatccgatc acacagaagc ttacggatat actccggttc aagcacctcc
     2941 agatccggac caaaggagag caaatatccg taaacccatt catcctcggg aaactgttgg
     3001 ttcatttcca gactcccgtc ttcatttcgt ttgatccttg tctcatcaaa gtcgtcgtaa
     3061 aggcggtagg cggcaccagg cttgaaccgc aaccgcaaat caacccaatc cctttggtca
     3121 ggcaggtttt cctccgagtt ttttatgtct aaaccatcat ctcttctggt gaagttttga
     3181 tccgtcagaa ccacatcccg catccgggtg atccggaagg tccgaaaggc gcttcggctc
     3241 cggcaccacg cccaaagata ccacgctctg gccttaaaga gcaacttcat cggttccacc
     3301 attcggtgac tccgtttccc gttagcgccg agatattcaa actccaatac caggcgctcc
     3361 aatattcccc ggcgaaccgt ttgaaacttg agcttttcct tcgacccgct gccccagggg
     3421 gagaagtcga tctccaccca atcggcggcg gcggcatttt taaagattgc cccgagttta
     3481 tcgagaacca gatcgacttc gggataattc accgcctgca gggtctttaa agccaataaa
     3541 atgctttcac tctcccgatc ggttacgatg gcccggtcaa gggagaaatt ctccatgatc
     3601 gatatcccgc cgccgtttcc ctgatgggca tagaccggaa cgcccgcagc cgacagatct
     3661 tcgatatcac ggtagatagt ccgggtggaa acctcaaacc gctccgccaa ctctttcgcc
     3721 gtggtcgttc ctttgttaag caagatggtt gtaatcgcca ccaagcgatc gatcttcatc
     3781 tcaaaatcag cgctccttca cgggatggat cctagctact accatattac accaaaaatt
     3841 ggacatcagg ttgtcatatt taaaaaccgt atcatttttg tgctacaata aattgttgaa
     3901 attttcgaga gctcaaatca atcttttcat gggggaaagt atggaaaagg caatcgtccg
     3961 gaatggaatt gataatatcg ccaaatatag tgaactcttt gccggaaaac gggtggggtt
     4021 gataaccggg cctaccggtg tgaacaagca attgaaatcg acgattgata tctttgccga
     4081 aatgttcaat ttaaccaccc tctacgcacc cgaacatggc atccgcgggg agcggcaagc
     4141 aggcgcaacg gttgaaactt accaagacga gcgaacaggg ttaacagtat acagcctgta
     4201 tgggatgaat aagaagccct cttcggaaat gttggcgaat gtggatattc tggccatcga
     4261 tatccaggat gtcgggatca ggtattacac ctatctct
//
LOCUS       DASVMB010000177         6676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_538818,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000177 DASVMB010000000
VERSION     DASVMB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538818"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF531_05290"
     CDS             <1..300
                     /locus_tag="VF531_05290"
                     /inference="COORDINATES: protein motif:HMM:NF020122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HEX7713415.1"
                     /translation="NRYGEGVAYYCGTYGEPAFYQFFLKTVLAEVDLSGLIDLPEGVD
                     YCKRRKDGMDYLFLVNRTAEPKVCRLPAIFSLVLGEKPDGDRLTLAGYGVGIYKS"
     gene            complement(416..817)
                     /locus_tag="VF531_05295"
     CDS             complement(416..817)
                     /locus_tag="VF531_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7713416.1"
                     /translation="MDEVIRRLKEGPYGSLATVDNGRPRVRPFGFMYEEEGKLYFCTN
                     SNKEVYKQLQAAPYVEYSVTSRDMITVRISGEVKFCEDRSKKERALNASELVKRIYQS
                     ADNPIFKVFYIEHGSVTVSDLSNQSQRKLEF"
     gene            960..1334
                     /locus_tag="VF531_05300"
     CDS             960..1334
                     /locus_tag="VF531_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017151857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713417.1"
                     /translation="MTDQANEMKPCPVSVTQNILMGKWKLMILWIVSQKTRRFNELQR
                     LMPKVARGVLTQQLRELERDKLIHREVYKEVPPKVEYSLTEIGRSFIPVMMQIMEWGI
                     EYMKQTTDCNMDICLSNKFSCY"
     gene            complement(1392..2288)
                     /locus_tag="VF531_05305"
     CDS             complement(1392..2288)
                     /locus_tag="VF531_05305"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX7713418.1"
                     /translation="MNGYSTPVYEERINRVLDYIEQDMDKPISLQSLAETACFSPYHF
                     HRIFTAWIGETPIDYVRRIRLEKAAMLLVHKKSVSITEIALQCGFSSSSVFSRTFQNH
                     FHTSPRAYRQKHPGKLEDALNEPYRKPSQRPVPGLTLSDPVKLEHFPAYAAVFARYQG
                     GYNKGIGSIWGRLMRWAFAHNVHGSTAFGIPLDDPEITPAAKCRYLACLAINPEIAKT
                     LKLPGYLSLMEIPGGLYAVSHFEGEEKNITRAYNGLYGDWLPASGFEPADSPALEVYY
                     SDARERAAGKFRFDIRLPLKPL"
     gene            complement(2491..3480)
                     /locus_tag="VF531_05310"
     CDS             complement(2491..3480)
                     /locus_tag="VF531_05310"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HEX7713419.1"
                     /translation="MLNAMRYLGYPVNETMIVGGGGALGFDYQKGIFPFLGARSQNMR
                     EVFAGGSSIKWHAVKPATPEESWEGVVELLQRGIPVVLRVDMRYLPYRYGGKYGPSYM
                     SFGWHMITLFGMDLEKGMAYVSDREYDTIQAIRLQDLDKARASKTKVFPPCREFSWVE
                     PMPQNYKLNWDKLTRNAVHQLVQNLEPSSSGLGGLNDLKALPDNIRRIETYATNRWLL
                     PAIFSSLHGYIEDFGTGGAAFRIMVRDFFEEASGSLTTTDFEAAITALNESITAWHGL
                     SGEFETISKQIKNVQTKSARDALYNKAAEQAEILYKKESSLLMALKKIDKSQE"
     gene            complement(3491..3676)
                     /locus_tag="VF531_05315"
     CDS             complement(3491..3676)
                     /locus_tag="VF531_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713420.1"
                     /translation="MNPRASMEAVWFEQKKYQIHAKGVGNDVTVDLLRLWKTMGCPKE
                     SGKNNLAECASKEGGSL"
     gene            4027..4896
                     /gene="dapA"
                     /locus_tag="VF531_05320"
     CDS             4027..4896
                     /gene="dapA"
                     /locus_tag="VF531_05320"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7713421.1"
                     /translation="MQLSGVWLPIITPFADDAIDYESLRKMIDHYIGQGISGLIPLGT
                     TGESPTVTDSEYEEFIVRTMEFVNGRVPVIAGAGGNCTKKTVELVRKLEKYRIDGILS
                     VCPYYNRPDQNGIYQHFQKVAEATDLKIIVYNIPYRTGRNIENETIHKLAEISNIVGV
                     KDSCGDFKQSMALLLNPPEDFAIFTGEDILFYPTLMLSGAGGILASAHLATADFVGLY
                     NLVQAGDRLGALTKWRKLTEFIPLLFAEPNPAPIKYCLKQMELIRSEEVRLPLTPISS
                     GLRAKLDRVMGDL"
     gene            complement(5012..6124)
                     /locus_tag="VF531_05325"
     CDS             complement(5012..6124)
                     /locus_tag="VF531_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7713422.1"
                     /translation="MSNATPTRLAVIGGFLGAGKTTAILDIARIMVENGMRIGIVTND
                     QGSELVDTRFLSETGLPVLEVAGGCFCCNFKEFSAKLEQLADAERPDLILAEPVGSCT
                     DLIATIFRPLQHQLTRKFTLSPLSIIADPGRVRKLMLEPESSPFPNEINYLFRKQLEE
                     ADLILLNKVDTLTTEEETSLLRFLGSRLPWVEILPVSAKTGRGIPAWADKITGLQAGE
                     GRALAIDYDTYAAAEAALGWLNSSALLTGTKPLGINRMIREFLGELRREMHEEKLEIA
                     HLKVYGVSESDWVKASLTSTDGPIELNREMTSRPTQINLIINARVLVDPGRLRQLVAT
                     NLERIAANYKATLSDLKTESFSPARPRPTFRMAANS"
     gene            complement(6117..6452)
                     /locus_tag="VF531_05330"
     CDS             complement(6117..6452)
                     /locus_tag="VF531_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713423.1"
                     /translation="MAVKIHLQLISLRDPCTACGITKNLQLEILDRMAQVNKTVALEE
                     IMLDHPREANRVEGLEVEKFPALLIDGEQISAGSLIPRAQLEQLVQSRLSRKSDTDPS
                     EQRRGEVNE"
BASE COUNT         1640 a         1592 c         1665 g         1779 t
ORIGIN      
        1 aaccgttacg gcgagggagt cgcctattat tgcgggacat atggtgaacc cgctttttat
       61 cagttctttc tgaaaacggt ccttgctgag gtggatctga gcgggttaat agatctcccg
      121 gagggagtgg actattgtaa acggcggaaa gacgggatgg attatctgtt tctggtgaat
      181 cggactgcgg agccgaaagt ctgccggtta cctgctatat tttcactggt gttgggtgaa
      241 aaacccgacg gcgaccggtt gaccctggcc ggttacgggg tcggaattta taagtcataa
      301 tatttgctcg aggtttttgg cagaaaagcc ggggacccat atgaacccgg cttttttaac
      361 atttttatgt gtccaaggct atatgaaaag tcattttgta ggttccctgg attttttaaa
      421 actcaagctt cctctgggat tgatttgaaa gatcggaaac tgtgacactc ccatgttcga
      481 tatagaatac tttaaatatc ggattgtcag cggattgata gatgcgctta accagttccg
      541 atgcgttcag agccctttct tttttgcttc tatcctcaca aaacttcact tcaccgctga
      601 tcctcacggt tatcatgtct cgcgatgtaa cggagtactc gacataagga gcagcttgca
      661 gctgtttgta gacttctttg ttgctgttgg tacagaaata gagcttgcct tcttcttcat
      721 acataaaccc gaaaggccta actcttggtc tcccattgtc gactgtcgcc agagaaccat
      781 acggtccttc ctttaggcgc ctaatcactt catccatttt cattacctcc gaactcaatt
      841 tattcttgaa cttcctaaat atttcaaata atatattatt ataaatatcc gtttttacaa
      901 gtacgcatat ttaagttaca tagtcatata aaacggacta ttgagaaagg acgaggttaa
      961 tgactgatca agcgaatgaa atgaaaccgt gcccggtctc ggtgacgcaa aatatcttga
     1021 tgggcaaatg gaaattgatg atcttatgga ttgtaagcca aaaaacccgg cgatttaatg
     1081 agcttcagag gttaatgccc aaagtggccc ggggtgtact gactcagcaa ttaagggaac
     1141 tggaaaggga taagctgatt catagggagg tgtataagga agtaccgcct aaggttgagt
     1201 attcattaac cgaaatcggc agaagtttta ttcccgtaat gatgcagatt atggagtggg
     1261 gcattgaata tatgaaacag accactgatt gtaatatgga tatttgtttg tccaataaat
     1321 tttcgtgcta ctaatgttat gagatgatga agaataagga acggcagaaa atagatctcc
     1381 tcggatgcgg ctcaaagcgg ttttagcggc agacgaatat cgaatcgaaa tttacccgcg
     1441 gcgcgctccc gggcatcact gtaataaacc tccagagccg gactgtcggc aggttcaaaa
     1501 ccgctggcag gcaaccagtc gccgtaaaga ccattatacg cgcgggtaat atttttttct
     1561 tccccctcaa aatgggatac tgcatataat ccgccgggta tctccattag gcttaagtat
     1621 ccgggaagtt ttaaagtctt cgcaatttcc gggtttatcg ccagacaggc caaataacgg
     1681 catttagcgg caggggttat ctccggatca tcaaggggga ttccgaaagc agtggaacca
     1741 tgcacattat gagcgaaagc ccaacgcatc agacgtcccc agatactgcc gattcctttg
     1801 ttataacctc cctgataacg ggcgaatacc gcggcgtaag cggggaaatg ctccagttta
     1861 accgggtcgg acagggtcaa ccccggaacc ggtctttgcg aaggtttacg atacggctca
     1921 ttcaaggcgt cctccaattt accggggtgt ttttgccggt aagcccgggg tgaagtatga
     1981 aaatgatttt gaaaagtccg ggaaaaaacg gacgaggaag agaaaccgca ttgcagcgca
     2041 atttcagtaa tggagaccga ttttttatga acgagcagca ttgcggcttt ttcaagacga
     2101 atccttcgta cgtaatcgat gggggtttcg ccgatccaag cagtgaaaat gcgatggaaa
     2161 tgatagggcg aaaagcaggc ggtttcggcc agggattgta aagaaatcgg tttatccatg
     2221 tcttgctcta tgtaatcaag aactcgattg atgcgttcct catatacggg cgtggagtat
     2281 ccgttcaata agattccctc cttgtaaacc actgggaata aacaacgttt catatcggtg
     2341 catcgctatc tgataaatgg ttatccattg tatgcgatgc gaatcattga tgacgtttta
     2401 tgtaattccc tgtgggggtg gaggaatcct tttttataaa attgtaggtc gcgtgcatgc
     2461 acgcgaccta ctactacaat tattttttca tcattcttgg gatttatcga tttttttcag
     2521 agccatcagg aggctcgact cttttttgta aagaatctcg gcctgttccg cagccttgtt
     2581 atacaaagca tcccttgccg atttggtttg gacgttctta atctgttttg atatcgtttc
     2641 aaactctccc gaaaggccgt gccaagcggt gattgattca ttgagagcgg taatcgccgc
     2701 ttcaaagtcg gttgttgtta gacttccgga tgcttcctcg aagaaatccc ggaccatgat
     2761 ccggaaagcg gccccgccgg tgccgaagtc ttcgatataa ccgtgaagac ttgaaaagat
     2821 tgccggcaat aaccaacggt ttgtggcata ggtctctatt ctgcggatat tatcgggtaa
     2881 cgcttttaag tcatttagac cgcccaagcc ggaggaagaa ggctctagat tttgtacaag
     2941 ctgatgaacg gcatttcttg tcagcttatc ccagttaagc ttatagtttt gcggcattgg
     3001 ctctacccaa ctgaactcgc ggcaaggagg aaaaaccttg gttttggaag cgcgtgcttt
     3061 atcaagatcc tgtaaccgga tggcttggat cgtatcatac tcccgatcag atacataggc
     3121 catgcctttt tcaagatcca tcccgaacag cgtaatcatg tgccaaccga aggacatgta
     3181 ggaagggccg tatttgccgc cgtaacggta gggaaggtaa cgcatgtcca cacgtaaaac
     3241 aaccggaata ccccgctgca acaattctac gaccccttcc catgattctt ccggggtggc
     3301 gggctttaca gcatgccatt tgatgcttga accgccggca aagacctcgc gcatgttttg
     3361 actacgggcg cccagaaacg ggaagattcc tttttggtaa tcgaatccca aagctccgcc
     3421 gccgccgaca atcatcgttt catttaccgg ataacccaga taacgcatgg cattgagcat
     3481 tgccgcgctt tcacagtgat cccccctctt tacttgcaca ttcggcaaga ttatttttgc
     3541 cggactcttt tggacaaccc atggttttcc acaaccggag caaatcaacg gtaacatcat
     3601 taccaacacc cttcgcgtga atttgatatt tcttttgttc aaaccaaacc gcctccatgc
     3661 ttgcgcgtgg attcacgatg gtttcattct aaactgaaat gggcatcagt tctttacttt
     3721 cattgccgtg tagtttgcaa ttcttgctct ttggttaaca gtatgctggt attgccgctc
     3781 cttaccgatc agacctgaac cgccggccca aagcgttcgg ttaaggcctt tccggttagg
     3841 aagtaattat ttctctaccg gtagaaggaa tcccggactc tgagaaggaa tatacagaat
     3901 caccagcatc ttggtttccg gagcgggtac ctgtgaaagc ggcgaattat ttcgacatat
     3961 caataaccat catcataata atggaagacg gttctgacat agaatgagtt gaaaggagtg
     4021 gttttaatgc aactcagcgg cgtttggctt ccgatcatca cccccttcgc cgatgacgcg
     4081 atcgattacg agtctttgcg gaaaatgatc gatcattata ttggccaggg gatctccggg
     4141 ctgatacctt tgggaactac cggggaaagt cctacggtca ccgattccga atacgaggag
     4201 tttatcgtcc gtaccatgga atttgtgaat ggacgggtcc cggtcatcgc gggtgccggg
     4261 ggcaactgca ccaagaaaac cgtagagttg gtacgcaaac tcgagaagta tcggattgac
     4321 gggattctct cggtctgtcc ctattacaac cggccggatc agaacggtat ctatcaacat
     4381 tttcaaaagg ttgccgaggc gacggattta aagatcatcg tctacaatat tccgtaccgt
     4441 accgggcgaa atatcgaaaa tgaaaccatt cacaagcttg cggagatttc caatatcgtt
     4501 ggtgtgaagg actcttgcgg cgactttaaa cagtccatgg cattgctgtt aaacccgccg
     4561 gaggatttcg ctatcttcac cggggaggat attctgttct accccacctt gatgttgagc
     4621 ggggccggtg ggattttggc ctcagcccat ctggccactg cggatttcgt aggactttac
     4681 aatctggttc aggcgggcga ccgtctgggt gccctcacga aatggcgtaa attgacggag
     4741 tttattccgc tccttttcgc cgaaccgaat ccggctccaa tcaaatattg cctcaaacaa
     4801 atggagctca tccgttcgga ggaagtccgg ctgccgctga cgccgatttc ttccggattg
     4861 cgggccaaac tggatcgggt gatgggggat ttataagaaa acgactcacc cccttccccc
     4921 tctttttcgg aaagagaggg ggaagggggt gagtcgcata agagttaatt tgctgaaatt
     4981 gcttttgtaa caagtaccaa aaccaattag actaactatt cgcggccatc cggaaagtgg
     5041 gtctggggcg ggcgggactg aaactctccg tcttcagatc actgagggtg gctttataat
     5101 tggcggcgat acgttccagg ttcgtggcga ccaactgccg gagacgtccc gggtccacta
     5161 acacccgggc attgatgatc aggttgattt gggttggccg gctggtcatt tcccggttta
     5221 gctcaatagg cccgtcagtg ctggtaaggc tggccttgac ccaatcgctc tcactcaccc
     5281 cgtaaacctt caaatgggcg atctccaact tttcctcatg catttcccgg cgtaattccc
     5341 cgagaaattc acggatcatt cggtttatcc caagtggttt ggttcctgtg agcagcgccg
     5401 aactgttgag ccatcccaag gcggcctcgg ctgcggcata ggtatcgtaa tcgatggcga
     5461 gtgcccttcc ttcacctgct tgcaggcccg tgatcttgtc cgcccaagcg gggatccccc
     5521 ggccggtttt agcggagacc gggaggattt ccacccaggg caaccggctc cctaagaacc
     5581 ggagcaggga ggtttcttct tctgtggtta gggtatcgac cttattgagc aggattagat
     5641 cggcttcctc cagctgtttc cggaagagat aattgatctc gttgggaaag ggcgaggatt
     5701 ccggttccag catcagcttt cgaaccctcc ccgggtcggc gatgatggag agcggactga
     5761 gggtgaattt ccgggtgagc tggtgttgca aaggccgaaa tatggtggcg atcagatccg
     5821 tgcaactgcc taccggttca gccaggatca gatcgggccg ttccgcatcg gcgagttgct
     5881 ctaacttggc gctgaattcc ttaaaattgc agcaaaagca gccgccagcc acttccagta
     5941 ccgggaggcc tgtctcagaa agaaatcggg tatccaccaa ctcgctccct tgatcattgg
     6001 ttacgatccc gatccgcatg ccgttttcca ccatgattcg cgctatatca agaatggccg
     6061 tggttttacc ggcgcccaga aaaccgccga ttactgccag ccgggtagga gttgcgttac
     6121 tcattcactt cgcctcgtct ctgttccgat ggatccgtgt cacttttcct actgagccgt
     6181 gactgtacta actgctccag ctgtgcccgg ggaatgaggg atccggcgct gatctgttcg
     6241 ccatcgatca aaagcgccgg aaacttttcg acttccaatc cctcgacccg gttagcctcg
     6301 cggggatgat ccaacattat ctcttccagc gcgacggtct tatttacctg cgccatccgg
     6361 tccaggatct ccagctgcag gtttttcgtg atcccgcagg cggtacaggg atcacggagc
     6421 gagattagtt ggagatgtat ctttacggcc atgatatcac tccattggca ataggcgtga
     6481 ggcatgagta ttgtaagtca tttactcgag tctaaggtcg gttgaaagtc gaaaggcgac
     6541 tcacccctct tgctcccctt atacggtaaa cttagattaa gatattcctc aaccgtaact
     6601 gagtaggggt cggggaagat ttggcagaat aataagtttc acctatgttc atcagaagca
     6661 aaacttcgtc ttacgt
//
LOCUS       DASVMB010000178         4721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_538989,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000178 DASVMB010000000
VERSION     DASVMB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538989"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF531_05335"
     CDS             <1..489
                     /locus_tag="VF531_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713424.1"
                     /translation="VNDLIVGLGGQAIHFTGDMGWFLPLVVLSNLWKDIGYGSIIYLA
                     AITQIDPQLYEAAKVDGAGRMAQLLHITLPGIMPMISMMLVMGIPNLINAGFDQVYLM
                     SNPMNLPVSEILETYVLKTGLSWGDFAKASAIGICNAIVALILVAMANKGSAKMGGST
                     IW"
     gene            519..1424
                     /locus_tag="VF531_05340"
     CDS             519..1424
                     /locus_tag="VF531_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713425.1"
                     /translation="MRVRNSSTTERAFNTINYTILALFVILIILPFWYISVVALNEGM
                     DTARGGVYLWPRAWTLDNFRAVFTDLSIIKYFFNSLLRLCLLTPLHLLVIGMAGWALS
                     RKNLPGRKFFAGMFVISMFVNPGVIPFYMTLVQFKLINNFWVYVLPWLFSTWELMILL
                     VSIRQIPESLIESAYLDGANDMVVFSRIIVPLTTATLVTLGLFNAVWAWGDWFTGAFY
                     IRSHDKWTIATYLQMVLQRGASMQVRSAAEASISSQNVAHQVTISETSLRAATLIVVI
                     FPIACVYPFLQKYLIHGVMVGSIKE"
     gene            1555..3243
                     /locus_tag="VF531_05345"
     CDS             1555..3243
                     /locus_tag="VF531_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713426.1"
                     /translation="MIKVLRKKWLFGVLLTLVVVQLSFALAAPKGIKTTIYNFGLQND
                     RLGRALELPNKNGEMTKWVNNKFGLELNYEYVLDANKQEQMTLWSASGEYPESFVYTT
                     HAAVFNWGRNMKYANLDPYYNDPKNFPRLYEIKHKYPRTLAPLTNEGHIVGFPTRMNY
                     KVGVPNPDIAYTFGWWIRDDILKSLGGVRPTTLDEFTEMLRKIKTGNFKSPSGKPVIP
                     LLLPPADWYSECTFFQTFGLNWIGMTKTGWYQFWGMTKQGYEAMKYANQLFNEGLIDP
                     DWVTQKDDMFQEKQRNGSAAVIVGWLPYTLIEDVKKAGYNFSYTALPVPKVPGVSRSY
                     PWNVLAGPDSDMVLYVTSKASKAQCDRLAKLAEWALTIEGARSLYLGASPDMIELKKS
                     GPYKGTYWFKDQFKDLDWYVNGDTRAARIKHGVVPLNGPVSYTRAELKVPDILGNQEK
                     WAKENYKWLSANGNIAYWGDVGPEFVPDEVVSINNRIWSEIPPMLLRACVLPADQFEA
                     TYKQAISTCKQVGFTKLCDDQWTRRKAYIDKNGGKKELMIPDDYPYADFLEEFK"
     gene            3392..>4721
                     /locus_tag="VF531_05350"
     CDS             3392..>4721
                     /locus_tag="VF531_05350"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX7713427.1"
                     /translation="MTFPQDFVWGAATAAYQVEGGAYEDGKGLSVWDTFCRKDGAIEE
                     RHNGDIACDHYHRYPEDVELMKRIGIGAYRFSISWPRILPDGTGPVNEKGLDFYDRLV
                     DSLLANGITPYVTLFHWDYPYELFKKGGWLNPDSPEWFSDYTRIVAQRLSDRVTQWFT
                     INEPQCFIALGHQDGVHAPGLKLGPGELLLASYHALLAHGMGVMALRNSSKQQCKIGF
                     NPLVTIRIPDSGSAADIEAARQATFSREDLNFWSNLFWLEPIFKGSFPAEIWNSPWGK
                     WMPEIKPDDLRIISQPLDFFGMNHYAGARVKAGEAGPQEAPRAPGYPRTMFDWAVTPE
                     SLYWGPKFFYERYHLPVMITENGMSNVDWVSEDGAVHDPQRIDFLHRYLRQFQKAAAD
                     GVEVKGYFCWSLLDNFEWARGYKERFGLIHVDFASGQRIIKDSGYWYSRVI"
BASE COUNT         1085 a         1205 c         1259 g         1172 t
ORIGIN      
        1 gtcaatgacc ttattgtggg acttggaggc caggcgattc attttaccgg ggatatgggc
       61 tggtttctcc ccctggttgt gcttagcaac ctctggaagg atattggcta cggttccatc
      121 atttatctgg ccgccatcac ccagatcgat ccccaactct acgaagcggc caaggtggat
      181 ggcgccggac gaatggcgca actgttacat attacattac cggggataat gcccatgata
      241 tccatgatgc tggtcatggg gatcccgaat cttatcaatg ccggattcga ccaagtctac
      301 ctcatgagta accccatgaa tctgccggtt tcggagattc tcgaaactta tgttctgaag
      361 acgggtttat cctggggaga ttttgccaag gcatcggcca ttggaatctg taatgccata
      421 gttgccttga tcttagtggc tatggccaac aagggatcgg ccaagatggg cggttcgacc
      481 atctggtgat cgagaccgga gatgaaagga gtgccccgat gagagttcgt aacagttcca
      541 cgacggaacg cgcctttaat accattaatt acaccatctt agctttattt gtcatcctca
      601 tcattctgcc gttttggtat atttcggtag tcgccttaaa cgaggggatg gataccgccc
      661 ggggcggcgt ttacctatgg ccccgcgctt ggaccctcga caacttcagg gcggtcttca
      721 ccgacctttc catcatcaag tattttttta attccctgct cagactctgt ttattgaccc
      781 cgttgcatct gttggtgatt ggcatggcgg gatgggctct ttcccggaag aacttgccgg
      841 ggcggaagtt tttcgccgga atgtttgtaa tatcgatgtt tgtcaacccc ggcgtgatcc
      901 cattctacat gacgcttgtc caatttaaac tgatcaataa tttttgggtt tatgtccttc
      961 cctggctctt cagtacctgg gaattgatga ttctattggt ttccattcgt caaatcccgg
     1021 aaagcctgat cgaatcggca tatctggatg gggccaacga tatggtcgtg ttttcgcgaa
     1081 tcattgtgcc gttgactacc gccactctgg tgaccctggg cctgtttaat gcggtctggg
     1141 catggggcga ctggttcacc ggcgcttttt acatccgcag ccatgataaa tggaccattg
     1201 ccacctatct ccagatggtt ttgcagcggg gagcctcgat gcaggtccgg agcgcagctg
     1261 aggccagcat cagttcccaa aacgtcgcgc atcaggttac catcagtgaa acctccctgc
     1321 gcgccgcgac actgatcgtt gtgatctttc caatcgcctg tgtctatccg tttctccaga
     1381 aatacctcat tcacggtgtc atggtcggct cgatcaagga gtgagtccgc gccggccgct
     1441 tccaatgaat tctgttaccg gttgaccggt ggccaggctg accggacaga agatatgaac
     1501 ggccttccgg ccgttcgatt gtgaaaaaac aaaattaaag gaggaagcga caagatgatc
     1561 aaggttttac ggaagaaatg gttattcggt gtgctactaa ccctggtagt cgtgcagttg
     1621 tcattcgctc tggccgctcc caaagggatc aagaccacga tctacaactt tgggctccag
     1681 aacgaccgtt tgggcagagc tctggagttg ccaaacaaga acggggaaat gaccaaatgg
     1741 gtaaataaca aattcggcct ggaactgaat tacgagtatg tgctcgatgc caacaaacag
     1801 gagcagatga cgttatggtc ggcttcgggc gagtatccgg aatccttcgt ctataccacc
     1861 cacgcggcgg ttttcaactg gggccgtaac atgaagtatg ccaatctcga tccctattat
     1921 aacgacccga agaatttccc gcgcctgtac gagatcaagc ataaataccc ccggacgttg
     1981 gcgccgctta ctaatgaagg ccacatcgta ggtttcccga cccgtatgaa ttataaggtg
     2041 ggcgtcccca atcccgatat cgcgtatacc tttggctggt ggatccggga tgatatcctt
     2101 aaatccctgg gcggagtgcg ccccactaca ctcgatgagt tcaccgagat gctccggaag
     2161 attaagaccg gaaacttcaa atctcccagc ggtaagccgg tgatcccgct cttgttgcct
     2221 ccggcggact ggtattcgga atgtactttc ttccagacct tcggattgaa ctggatcggc
     2281 atgaccaaaa ccggttggta tcaattctgg ggcatgacca agcaaggtta tgaggccatg
     2341 aaatacgcca accagctctt taatgaaggg ctgatcgatc ccgactgggt gacccagaag
     2401 gacgacatgt tccaggagaa gcagcgcaat ggttcggccg cggtcatcgt cggttggctg
     2461 ccgtataccc ttatcgaaga tgtcaaaaaa gccggttaca acttctccta tacggcgttg
     2521 ccggtgccga aagtaccggg agtctcccgg tcatacccct ggaacgtcct cgccggaccc
     2581 gattccgata tggtactcta tgtcacctcc aaggccagca aggcccagtg cgatcgtttg
     2641 gcaaaactgg ccgaatgggc tctgacaatc gaaggcgcgc gtagtctcta cctgggcgcc
     2701 tcgccggata tgattgagtt gaagaagtcc ggtccttaca aaggcaccta ctggtttaag
     2761 gatcaattca aagatctcga ttggtacgtc aacggcgata cccgcgccgc ccggatcaaa
     2821 cacggtgtgg tcccgctgaa cggaccggtt tcatacaccc gcgccgagct gaaggtcccg
     2881 gatatcctag gcaatcagga gaaatgggcc aaggagaatt acaagtggct tagcgccaat
     2941 ggcaatatcg cctattgggg cgatgtcggc ccggaatttg taccggatga ggtggtctcg
     3001 atcaataacc gcatctggtc ggagattccg cctatgcttc tgcgggcttg cgtcttaccg
     3061 gccgaccaat ttgaggccac ttataagcag gcgatatcca cctgcaagca ggtgggcttc
     3121 acgaaactct gtgacgacca atggacccgc cgcaaggctt acatcgataa gaacggcggc
     3181 aagaaggaat tgatgatccc ggatgattat ccgtacgccg acttcctaga ggagttcaaa
     3241 taagctcaac cgggtattca tttcgattac cagatttacc gtttgattag gacgttgcat
     3301 cgggactccg gttttgatgc aacgtcttat acgttatccc attctcgcag tggggttcgc
     3361 gcttgggcgc gtattaaagg agggcataat tgtgacattt ccacaggact ttgtatgggg
     3421 cgctgcaacc gccgcctacc aggttgaggg tggcgcgtat gaggatggga aggggctctc
     3481 ggtttgggat accttctgcc gcaaagacgg tgcaattgaa gaaagacaca acggcgatat
     3541 tgcctgcgac cattaccatc gttacccgga agacgtggaa ttgatgaaac ggatcggaat
     3601 cggggcatac cgtttttcca tctcctggcc caggatttta ccggatggaa ccggtcctgt
     3661 caacgagaag ggcttggatt tttacgatcg tctggtcgat agtttgctcg caaatggcat
     3721 cacaccttat gttaccttgt ttcattggga ttatccctat gaattattca aaaagggtgg
     3781 atggctgaac cccgacagtc ctgaatggtt cagcgactac acacggattg tggcacagcg
     3841 tctttcagac cgggtcactc agtggtttac catcaatgag ccgcaatgct tcatcgccct
     3901 cggacatcag gacggcgttc acgctcccgg actaaagctg ggtcccggag aactcttact
     3961 tgcgtcatat catgccttgt tggcccatgg aatgggagtc atggcgctcc gcaattcctc
     4021 aaaacaacaa tgtaaaatcg ggtttaaccc gctggtaacg atacgaatcc ccgattccgg
     4081 gtccgcagcg gatattgaag ccgcccggca ggctactttt tcccgggaag atttgaattt
     4141 ttggtccaac ctgttctggc tggaacccat atttaagggt tcattcccgg cagagatttg
     4201 gaacagtccg tgggggaaat ggatgccgga gatcaagccg gatgatttac gaatcatctc
     4261 gcagcccctc gatttcttcg gcatgaatca ttacgccggg gctcgagtga aggccggaga
     4321 ggcaggcccg caagaagcac cccgcgctcc gggatatccc cgaaccatgt ttgactgggc
     4381 ggtgaccccc gagagtttat attggggacc gaaatttttc tatgaacgtt accatcttcc
     4441 ggtcatgatc actgaaaacg ggatgtctaa tgtcgattgg gtttctgagg acggcgcggt
     4501 ccatgacccg caacgcattg acttcctcca tcgttatctg cgtcaatttc agaaagctgc
     4561 agccgacgga gtagaggtta agggttactt ttgctggtca ttactggaca attttgagtg
     4621 ggcccggggt tataaggaac gtttcggcct gatccatgtc gatttcgcct ccggacaacg
     4681 gattatcaaa gactcgggtt attggtacag ccgggtgatc g
//
LOCUS       DASVMB010000179         5551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_99205,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000179 DASVMB010000000
VERSION     DASVMB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99205"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..674
                     /locus_tag="VF531_05355"
     CDS             195..674
                     /locus_tag="VF531_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713428.1"
                     /translation="MNEVSKPFRYTYSTIFTAKMRNFLKKSIWIYFAASAIISCLLWG
                     VNAGACLLFLGIFGVLILISLTAMTLSTFFIYQRKKLDDMVISFREPDIEILWENKNI
                     RETKNWDWIQKVKERENVYYLDLAVWPKNTLMIDKSRLSIEEDELLHRLFKEHQKVG"
     gene            783..923
                     /locus_tag="VF531_05360"
     CDS             783..923
                     /locus_tag="VF531_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713429.1"
                     /translation="MTSAGISAGIDLSLHIVEKLYGEEIKNQTVVYMEYGNWRLNNGI
                     EG"
     gene            930..2066
                     /locus_tag="VF531_05365"
     CDS             930..2066
                     /locus_tag="VF531_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7713430.1"
                     /translation="MEYRKLGKKGLEIGVIGLGTEFIWHEPQDVVTEVVHEALDHGVN
                     YFDLWMPSPEIRDYFGVAIQGHREKINIAGHLGSTLKDGQYFRTRDLQKSEAFFDDLL
                     KRLRTDYVDILMLHFIDDEEDYKAVFESGEFYELALRLKSTGKARYIGMSGHKVPMAL
                     KAVQSGKIDLLMFPVNPAFDSLAGEMGLEAFWDEKTYQNEATAVSLARKELYHLCEQQ
                     GVGIVAMKPYAGGWLLNGKTNDMNLSPVQCLSYCLAQPGVVTAIPGCKNIDEMKQALA
                     FLNATENEKDFSILNSSTRWNIKGVCMYCNHCLPCPAKIDIGKLTRLVTTAELGLSEG
                     IRAKYQSLEFKASDCMKCGSCMKRCPFGVDVLSNMEKAVTIFGQ"
     gene            2082..2867
                     /locus_tag="VF531_05370"
     CDS             2082..2867
                     /locus_tag="VF531_05370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7713431.1"
                     /translation="MEIDWNEESKRFDEAADYYDVYRPSYPQAVIDWLIENAGIRPQD
                     KLLEIGAGSGKATELFVERGYELVCIEPGENLAKTGLRKFSGSHRVSYVVTRFEEWDE
                     AENSFDFAFSAQAFHWVPKPIGFEKCARALKSRKYLALFWNWYPLGKDPVFNELTPVL
                     RDYQLAIPSSDDEIEARVVSETDQINQSRRFKNPAVFRFPWEKRYEAEEYIGLLRTGN
                     DYLRLQPSDREGLENGVRLTLHKNGGFIIRPYLGIAFLAQKLD"
     gene            2920..3225
                     /locus_tag="VF531_05375"
     CDS             2920..3225
                     /locus_tag="VF531_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HEX7713432.1"
                     /translation="MIYDILQLAGGVIMSFGQIPQIVQILKTKSAVDLNLKTYIMMFT
                     GISLMESYAINLVLKGSGGAFLITNTASLLASGVMIVLILMYGRAKRDHERKYGCKV"
     gene            3197..3391
                     /locus_tag="VF531_05380"
     CDS             3197..3391
                     /locus_tag="VF531_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713433.1"
                     /translation="MKGNTDVRCERPEPVYISPKYIKIKEDRLKYLEAAEITKYLTER
                     EEMGIRDCIDTVTTACNEGE"
     gene            3392..4309
                     /locus_tag="VF531_05385"
     CDS             3392..4309
                     /locus_tag="VF531_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7713434.1"
                     /translation="MKSNTNPWDLDIYLYGMTVLSTIHRLAGPYPEADSYQEIKETYR
                     LPGGETGNSAIILSNLGYKVKIDGPFLGEKTKFDIEDFCAKHEIDCSGMLYDPGFDGV
                     QDLVLIDGHTRTVFGKFAQYFGDGKRWSQPDRSAIGSAKIVSLDPFFGAESEMTADYC
                     AALGKKYVTIDCAPESKIHKNAAATVISNEYIKNNFPDEELQHLMKRYTAASDGLVIF
                     TFGAREILYCRKGDAMKSMVPFRVEVKSTLGAGDTFRAGVVYGVLNQMSDEQIVKFAA
                     ATAASVCMRFPMALDPPSLDEITDLAERG"
     gene            4329..4775
                     /locus_tag="VF531_05390"
     CDS             4329..4775
                     /locus_tag="VF531_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713435.1"
                     /translation="MTERVRLIKHDELTMLLSLYTYLNPEDPCLEPNLELEKHWREIL
                     GDPSLYYLVIEEDNRLVSSCTLAIIKNLTRSARPYGLIENVVTHPDYRNKGYGTAVLR
                     KAVAIAMEKNCYKVMLMTGRKGESTLKFYEKAGFHRGEKTAFVIRF"
     gene            4841..5374
                     /locus_tag="VF531_05395"
     CDS             4841..5374
                     /locus_tag="VF531_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713436.1"
                     /translation="MTEQQKKSTWFQFLWRISASHIVSYFIMGVIFSTLLNYKANFAS
                     AALSFMRPTDSPWVAAGPSLQILRGILFALVLWPIHEVFLQRKKGWVTLWLLFLGLAI
                     LGTAGPTPGSLEGLIYTKIPIGYQLLGLPEVVLQTLLFSLFVFYWYKKPAKTWNVFMA
                     IGVGLIVLMSLAGVFLG"
BASE COUNT         1585 a         1056 c         1410 g         1500 t
ORIGIN      
        1 ctttatgaga tgaagttgga ttgattttta tggtatccag gaaggagtct gtactccgtt
       61 ataatgccta tgtctctttt tatgagaggg taaattagtt atcagactat ttgataaatt
      121 tagaagagat tacttgacgg caaacatttg ttcgaattat aattgaatca ccggataagg
      181 cgagggtgaa cgctatgaat gaagtgagta aaccattcag gtatacttat tccaccatct
      241 ttacagctaa gatgaggaat tttttgaaaa agagcatctg gatatatttt gcggccagtg
      301 cgatcatcag ctgccttctt tggggtgtga acgccggcgc ttgtctttta ttccttggaa
      361 tatttggcgt cctgattctt atcagcttga cagcaatgac cttatcaact ttttttatat
      421 atcaaagaaa aaagctggac gacatggtta tttcatttcg tgaacccgat attgagatat
      481 tgtgggagaa caaaaacatt cgggaaacga aaaactggga ttggattcag aaagttaaag
      541 aaagagaaaa tgtctattac ttggacttgg ccgtttggcc taaaaatacc ttaatgattg
      601 ataagtcgag actatcaatt gaagaagacg aattattaca tcggttattc aaagagcatc
      661 aaaaagtggg ttagtgttct tcttatatag gcagccccat aggtagaata aaacaggcat
      721 tggttaaatc cgaccaatgg aaggtaaatc atgattacga agatgatcag cggggcaaag
      781 tgatgacatc cgccggtatc tcggccggta tcgatctgtc ccttcatatc gtggaaaagt
      841 tatacgggga agagatcaag aatcagaccg tggtttatat ggaatatggg aactggcgat
      901 tgaataatgg aatcgagggg tgaatgcaat tggaatatcg aaaacttggc aaaaaaggcc
      961 tggagattgg agtcatcggt ttaggaaccg aatttatatg gcatgaaccg caggatgtag
     1021 tcacagaagt agtccatgaa gcccttgatc acggagtcaa ttattttgac ctctggatgc
     1081 cgtctcccga gattcgtgat tacttcggtg ttgccattca aggacatcga gaaaagatca
     1141 acattgccgg gcatctcgga tcaaccttga aagacggaca atatttccgt acccgcgatt
     1201 tacagaagag tgaagctttc tttgatgatt tattaaaacg gttacggacg gattatgtcg
     1261 atattctgat gcttcatttc atcgacgatg aagaagatta caaggcggta ttcgagtcgg
     1321 gggaatttta cgagctggca ttgcgtttga aatcaaccgg gaaagcccgc tatatcggaa
     1381 tgagcggtca taaagttcca atggctttaa aagcggttca aagtggcaag attgatctct
     1441 taatgtttcc ggtcaatccg gcttttgatt cattggcagg ggagatggga cttgaagcct
     1501 tttgggatga gaaaacctac caaaacgaag cgactgcggt aagccttgcc cgaaaggaac
     1561 tatatcatct ttgtgaacaa cagggcgtgg ggattgtcgc aatgaagccc tatgccggag
     1621 gatggctcct caatggaaaa accaatgata tgaatctctc acctgtccag tgcttaagtt
     1681 attgcctggc tcagccgggt gttgttacgg ccatacccgg atgtaaaaat atcgatgaaa
     1741 tgaaacaggc cctcgctttt ttaaacgcca ccgaaaacga gaaggatttc agcattctaa
     1801 actcatcaac ccggtggaac attaaggggg tttgtatgta ttgcaatcat tgtctgccat
     1861 gtcctgctaa aatcgatatc gggaagctta cccgcctagt aaccacggcg gaattgggat
     1921 taagcgaagg tatccgggcc aaatatcaat cccttgagtt caaggcctcg gattgtatga
     1981 agtgcggttc atgcatgaag agatgtccat tcggagtcga tgtcctttca aacatggaaa
     2041 aagccgtcac aatcttcggg caataaccct tgggggtaat tatggagatt gactggaacg
     2101 aagagagtaa gcgttttgat gaggcggcgg attactatga cgtataccgg cccagttacc
     2161 cgcaagccgt gattgattgg ctgatcgaaa acgcagggat tcgcccccag gataagttgc
     2221 ttgagattgg ggccggaagc ggcaaagcca ccgaattgtt tgtggaacgc ggttatgaac
     2281 tggtctgcat tgaacccgga gagaatctgg ctaagaccgg gctacggaag ttttccggat
     2341 ctcatagggt ttcctatgtt gttacacggt ttgaagaatg ggatgaagct gaaaacagct
     2401 ttgattttgc cttttcagcg caggcgtttc attgggtgcc taaaccgatc ggttttgaaa
     2461 aatgcgcccg ggcgctgaaa tcccggaaat atttggcact gttttggaat tggtatcctt
     2521 tagggaaaga ccctgttttt aatgaactga ctcccgttct aagagattac cagttagcca
     2581 ttccttcctc tgatgatgaa atcgaggcca gggtcgtatc ggaaaccgat caaatcaatc
     2641 aaagccggcg tttcaagaat cccgcggttt ttcgctttcc ctgggaaaag aggtacgaag
     2701 ccgaagaata tatcggttta cttagaacgg gaaatgatta cttgcgtctg caaccttccg
     2761 acagggaagg gctggaaaat ggggttcggt tgacacttca taaaaatgga ggatttatta
     2821 ttaggccata tctgggaatc gcttttttag cacaaaagct tgactaaaaa gttgtcagtc
     2881 aatctagggt tagatattaa agaatcacgg gagataaaga tgatctatga tattttacag
     2941 cttgcaggtg gagtgattat gtcattcgga cagataccac aaatagttca gatcctgaaa
     3001 acaaaatcgg ctgttgattt aaatctgaaa acctatatca tgatgtttac aggtatctct
     3061 ttaatggagt catacgcaat caaccttgtt ttaaagggtt ccggaggcgc gtttcttatc
     3121 acgaatacgg cttctttgct cgcgtcgggt gtaatgatag tgcttattct catgtatggg
     3181 agggcaaaaa gagatcatga aaggaaatac ggatgtaagg tgtgagagac cggagccggt
     3241 atacatcagt ccaaaatata taaaaatcaa ggaagatcgg ctaaaatatt tagaagcggc
     3301 ggagatcaca aagtatctga cagaaagaga agaaatgggc atcagagatt gcatcgacac
     3361 ggttactaca gcatgtaatg aaggtgaatg aatgaaatcg aacacgaatc catgggacct
     3421 tgatatatac ttatatggta tgactgtatt gtccacgatc catcgtttgg caggacctta
     3481 ccctgaggcc gattcatatc aagagatcaa agagacgtat cggttgcccg gcggtgaaac
     3541 cggtaactcg gccattatac tttcaaacct gggatataaa gtcaaaatcg acgggccttt
     3601 tctgggggaa aaaaccaagt ttgatattga agatttctgc gccaaacatg agattgattg
     3661 ttcgggtatg ctttatgatc cgggttttga tggtgtacag gatttggtgc tcattgatgg
     3721 gcatacaagg acggtttttg gaaaatttgc acagtatttc ggtgatggca aaagatggtc
     3781 gcaaccggac cgctccgcca tcggttccgc caaaatcgtg tctttggatc cgtttttcgg
     3841 agcggaatcc gagatgacgg cggattattg tgcggcctta ggaaaaaaat acgttaccat
     3901 cgattgtgca ccggagagca aaatccataa aaacgcggcc gctacggtaa tttccaacga
     3961 gtatatcaag aacaattttc ctgatgaaga gttgcagcat ttgatgaaac gatacacagc
     4021 ggcatctgat ggacttgtga tatttacctt tggcgccagg gagattcttt attgccgcaa
     4081 aggtgatgcc atgaaaagta tggttccgtt tcgggttgag gtaaaaagta cactgggggc
     4141 cggcgatacc tttcgggcgg gagttgttta cggagtccta aatcaaatga gcgatgaaca
     4201 gatcgtgaaa tttgcagccg caactgcggc tagtgtctgt atgaggtttc cgatggcgct
     4261 tgatccgccg agcttggatg aaattactga tctggcggag cgcgggtaac cttggaaagg
     4321 aggggggctt gactgagaga gtgcgcctta tcaagcacga cgagttaacg atgctcttat
     4381 cgctatatac atatttaaac cccgaagatc cctgcctgga gccaaatctg gagcttgaga
     4441 agcactggcg ggagatactc ggcgatccca gcctgtatta tttggtgatc gaggaagata
     4501 acagacttgt atcgtcatgt accttggcaa tcatcaaaaa cttaacgcga tcggcccggc
     4561 cgtacgggct cattgaaaat gtcgtcaccc atccggatta ccgtaacaag gggtatggaa
     4621 ccgcggtatt gaggaaagcc gtggcgatag cgatggaaaa gaattgctat aaggttatgc
     4681 tgatgaccgg gaggaaaggg gagagcacct tgaaatttta cgaaaaggcc ggatttcata
     4741 ggggtgaaaa gacggctttc gtaatacggt tttgaagcat aacaattaga ccgtcaccaa
     4801 aacaaattgc ttttttatta atcttaaaag ggggatccat atgacagaac aacaaaagaa
     4861 aagcacttgg ttccagtttc tttggcggat ttctgcctcc cacattgtat cgtattttat
     4921 catgggggtt attttttcca ctttattaaa ctacaaagcg aatttcgctt cggccgcgct
     4981 atcctttatg cggcccaccg attctccatg ggtggccgca gggccttccc tgcaaatcct
     5041 ccggggtatc ttatttgcac ttgttttatg gccgattcat gaggtgtttc tgcaaaggaa
     5101 aaagggatgg gtaaccttat ggctcctctt tttaggattg gcgatcttag gaaccgccgg
     5161 accgactccc ggttctctcg aaggattgat atacaccaag attcccatcg gatatcagct
     5221 gctgggttta cccgaagtgg tattgcagac acttctgttt tccctgttcg ttttttattg
     5281 gtataagaag ccggcgaaaa catggaatgt ctttatggcg atcggtgttg ggttaattgt
     5341 actcatgagt ttggcgggtg tatttttagg ataatgatgg aactgtaaag ataagaacgc
     5401 catatttttt caattgcgtg atcaagagaa aactccctta agaaggtgag cctatgagca
     5461 ctccactgat tttagatgga aaaacgttgg cacgacagat agaagccgat ctagagtccc
     5521 gggtagccaa gctaaaggag caggggaacc c
//
LOCUS       DASVMB010000180         5654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_169361,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000180 DASVMB010000000
VERSION     DASVMB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169361"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VF531_05400"
     CDS             <1..707
                     /locus_tag="VF531_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713437.1"
                     /translation="SLKEVDPPGNYLRKIRTFYESYEKELKIRPLLGEVDSLSRSLGH
                     YDPSNSKATATFAVRWNSMVQAFENLCLETVSRQRIGDLQQLLETLQNAIYEMQEQAN
                     LPGWPELISQTQNELSKLYELKKYPKNALAKFNLAQQYTTTFQTEKPPVHKLLTEDSE
                     IQSQIAQMNKCFLRLVNSINQKLPAPQRNLKIKPSEEDFSPAQDYRLVVSYHYNGNDD
                     EEFDTNFYAMLRNMIR"
     gene            complement(864..2228)
                     /locus_tag="VF531_05405"
     CDS             complement(864..2228)
                     /locus_tag="VF531_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713438.1"
                     /translation="MKRDKSIIQRLLLGLLIMASLSFATLIEASGSSTTPAPGNRSFR
                     SQREMVYALVLFDGGKYQETFSPSEIDTLYMMAGVNNVVSARKTDVYYWPLTKEYKAD
                     WMESNFPQNGTLEIMKGNKLFKLFRPAEYVLYYPQGTSEGATEVKTGSAITAIVQKYK
                     SDMAQYYQKVLDYYKEYERYNQLMARYANEPQKPGNIRPVEPKSPNPPSWYLTQPDQA
                     FLLNLPQGQYILRFKDQKGRVVPRSERKLICFGPSARGIGYEIIPRDKWTYPMNSNDQ
                     SEKILVLKTQAIYLKLFVELQFNEHHYTKIAQLASPSSGKGLENRLRWVHVSPMPATR
                     YQVAIFKNGKLIGKVREKPYFVKQRPGYALGYDIVEFIPKDHPGIAPNFTAFKIDMPK
                     DSGSYFLYLLDANGKAVPGSERNVRIVKKTGLPLLLSIFFLPLVAGAGIVGTRLMKIR
                     NSQC"
     gene            complement(2228..3049)
                     /locus_tag="VF531_05410"
     CDS             complement(2228..3049)
                     /locus_tag="VF531_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713439.1"
                     /translation="MNIRIKQIFLIGRKGFHTAFYSFGLYFVIVIGYILAGLAVNTYI
                     QTITEEGLLINSKPVSLPLYLAVMVGAIYLAIAASVSISRERESGTLEVLFYGPVDVV
                     SYVSGKFLEQLWLSGFMLLFYILAMLIFCFLTNIGSLLDVLISAVFFWGLTFSIISFG
                     ILISSITKKIRVSILWLVGLFILFLAISWGDSLTSGLDPQVIPEIFLYPMSMLSILAK
                     ITNWISPFAYMDRGMQALSVWNVVSCITSLLYSWFYSLVLLGLAIFSLQRKGVRG"
     gene            complement(3046..3993)
                     /locus_tag="VF531_05415"
     CDS             complement(3046..3993)
                     /locus_tag="VF531_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713440.1"
                     /translation="MIKIDGLTKEYDGERVVNGLSIHVEKGEIYGFLGPNGAGKSTTI
                     RMMLGLLPPTNGQVRIFGRTLQEDYFGIKRNVGVVGEVQHLYDEMTGFEYLSFFADFF
                     GVENKRVRIENLLKKVDLYRYHNLRLKEYSKGMQQKIGIIRALLHNPDLLILDEPVSS
                     LDPYGIKQVRDMIREENEKGKTFFLSSHLLSEIERTCHRVGIINRGMLVAEDTIEGIK
                     GKLSESYNLEVEVENCLPDTEGLLRELGFVDSVMIHGRIIRICTKDNGDYRVKISKFL
                     AEHGYVVLGVNKKELSLEEAFITLTEKNVSLLTGKGGTI"
     gene            complement(4292..5578)
                     /locus_tag="VF531_05420"
     CDS             complement(4292..5578)
                     /locus_tag="VF531_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7713441.1"
                     /translation="MNSGSKIVSVTAREVYSQRRHFGVEATIVTENGSVGVAVATGGT
                     SFGGNEAKFVYDQDQRYLGKGVKRAVDNVNKIIGPALKGRDTSKQWEIDQLLLELDGT
                     PDKSNLGANATGSVSAAALKAGAASLGIPLYQHIGGINACVLPVPGVLTVLGAWRHGA
                     GKKSGCKPTYSLMCYGFDSYYEASLAGWRAFSEYLNVLNRKLNLHYSLEFYDRIIINA
                     GIVKHDRELWEIMAETITNLGYEGKMGIQVDVAATTYYDAEKQKYVGLFSPEDKTKEE
                     LISLYKEMVKKYPFVIFEDPLEETDFEGFARLTKELGIEIVGDDLFASNIENVSKGAA
                     MGAANAVLLKVNQKGTITEAAEMIQYAYRCGYGVMPCSSRGEGVDIADYAVGFNTGHL
                     REGGIEETANRLLKIEAELGSRARFLGREGLRLAKK"
BASE COUNT         1578 a         1443 c         1126 g         1507 t
ORIGIN      
        1 tgtccttaaa agaagtagac cccccaggga actatctccg caaaatccgg actttctatg
       61 aaagttacga aaaggaacta aaaatacggc cactgctggg agaggtagat tccctctccc
      121 gttcactcgg tcattatgat ccgtccaatt ccaaagctac cgctaccttt gcggttcgct
      181 ggaattccat ggttcaagcc tttgagaatc tatgcctgga gaccgtctcc cgtcagcgta
      241 tcggcgatct gcaacaacta ctggaaactc tgcaaaacgc catttatgaa atgcaagaac
      301 aggccaatct tccaggctgg cccgagctaa ttagccaaac ccaaaatgaa ctgtccaaac
      361 tttacgagtt gaaaaagtac cctaaaaacg cactggctaa attcaacctg gcacagcagt
      421 ataccaccac attccagaca gaaaagccgc cggttcataa gcttcttacc gaggattccg
      481 agattcaaag ccagattgcc cagatgaaca aatgtttttt acgcctggtc aattcgatca
      541 accaaaaact cccagccccc cagcgaaatc ttaaaatcaa accctccgag gaggacttct
      601 cccccgccca agattaccgg ctggtcgttt cctatcatta caacgggaat gacgatgagg
      661 aattcgacac aaatttctac gccatgctgc gaaatatgat acggtaatcg gatattagct
      721 aaaaataaga gaggttgacc caaaagtatt tgggccagcc tctttttcgt ttttggtcgc
      781 gaattttctc aacgactctg ccgcacattc ttagggtttt ctacccgaac tcttctcgaa
      841 ccctcccttt tgctccctaa attttaacac tgagagtttc tgatcttcat cagccgtgtt
      901 cccactatac ctgcaccggc aacaagtggt aaaaagaaga tgcttaagag gagggggagt
      961 ccggttttct ttacaatccg cacattacgc tcactgccgg gaaccgcctt tccgttggca
     1021 tcgagtagat aaaggaaata actccccgaa tctttcggca tatcgatctt gaacgctgta
     1081 aagtttggcg caattccggg atgatccttc ggaatgaatt ccacaatgtc atagcccaag
     1141 gcataacccg ggcgctgttt gacgaaatac ggcttttccc gcacttttcc aattaatttc
     1201 ccatttttaa atatcgctac ttgataccgt gttgcaggca tcggcgaaac atgaacccac
     1261 cgcaacctgt tttcaagacc tttaccgctt gatgggcttg ccagttgggc aatttttgta
     1321 taatgatgct cattaaactg aagttcgaca aaaagtttaa ggtaaatagc ctgagttttc
     1381 aagaccaaaa ttttctcgct ttgatcattg ctgttcatcg gataagtcca tttatccctg
     1441 ggaatgatct cataaccaat tccccgggct gaaggcccaa aacaaatgag cttccgttca
     1501 ctgcgaggaa cgactctccc tttctggtcc ttaaatcgta gtatatattg accctgaggg
     1561 agattcaaaa gaaacgcctg atccggttgg gtcagatacc aactgggcgg gttcggcgac
     1621 ttcggctcaa cagggcgaat attccctggt ttttggggtt cattggcgta tcgagccatc
     1681 aattggttat aacgttcata ctctttataa tagtccagta ccttttggta atactgggcc
     1741 atatccgatt tatacttttg aacaatagcc gtaatggccg aaccggtttt aacttcagtg
     1801 gctccttcac tggtgccttg gggataataa agtacatatt ccgcgggccg aaaaagtttg
     1861 aacagtttat tccctttcat aatctctagg gtaccgtttt gcgggaaatt gctctccatc
     1921 cagtctgctt tatactcttt ggtaagcggc caatagtata catctgtttt ccgggctgag
     1981 actacattgt tcacaccggc catcatatag agggtatcga tttccgacgg tgaaaaggtt
     2041 tcctgatatt tgcctccgtc gaataatacc aaggcgtata ccatctctct ttgggaacgg
     2101 aaacttcggt tccctggggc cggggtcgtc gaactaccgg atgcctcaat cagagtcgca
     2161 aaagataaac ttgccatgat caagaggccg aggagcaatc tttggattat ggatttgtca
     2221 cgcttcatta acctctaacc cccttccttt gcaggctgaa aatcgctaat ccgagtaata
     2281 cgagagaata gaaccaggaa tacaataaac tggtgataca gctgacgaca ttccatacgg
     2341 aaagggcttg cataccccga tccatatagg caaagggcga tatccaattc gttattttcg
     2401 ccaaaataga gagcatcgac atggggtaaa ggaaaatttc cggaatcacc tggggatcaa
     2461 gaccggaggt cagtgagtcc ccccaactga tggctaaaaa cagaatgaac aatccaacca
     2521 gccacaaaat agatacccga attttcttgg tgatggaaga tatcaatatt ccgaaggaaa
     2581 taatactaaa tgtgagtccc cagaaaaata ccgcactgat caggacatcc aagagagaac
     2641 ctatattggt taaaaaacaa aaaattaaca tggcaagaat ataaaacaac aacatgaatc
     2701 cagataacca gagttgttca agaaacttcc cgctcacata ggaaacgaca tcgaccggac
     2761 cataaaaaag cacttcgagg gtacccgatt cgcgttcgcg ggagatggaa accgaggccg
     2821 caatagccag atagatcgct cctaccatga ccgccaggta caaaggcaaa ctgaccggtt
     2881 ttgaattaat cagcagccct tcttcggtaa tggtttgaat ataggtattc acggcaagtc
     2941 cggctaaaat gtaaccgatc actatcacaa aatacagccc aaagctgtaa aaagcagtat
     3001 gaaatccctt cctgccgatg aggaatatct gcttgatcct gatgttcata tcgtccctcc
     3061 ctttcctgta agcaatgaaa cattcttttc agtaagggtg ataaacgcct cttccaaact
     3121 gagctctttc ttgttgacac ctaaaaccac ataaccgtgc tctgctagaa acttggagat
     3181 tttcacccgg taatcaccgt tgtctttagt acaaatccgg ataatccggc cgtgaatcat
     3241 tacagaatcg acgaaaccta attcccttaa taacccctcc gtatccggaa gacagttttc
     3301 tacttcaact tctaaattgt aactctccga cagcttgcct ttgattcctt caatcgtgtc
     3361 ttccgccacc aacatcccgc ggttaataat ccccacccga tggcaggtcc tttctatctc
     3421 cgatagcagg tgggaggaga ggaagaaggt tttgcccttc tcattttctt cgcgaatcat
     3481 atcacgaact tgtttgatac cgtatggatc taaagatgaa accggctcat ccagaataag
     3541 caaatcggga ttatgcaata atgccctgat aatcccaatc ttttgttgca tacctttgga
     3601 gtattccttt agtcgcaggt tatgatagcg atacagatca acctttttaa gcagattttc
     3661 gattctcacc cgtttatttt caactccgaa aaaatcggca aaaaatgaga gatattcgaa
     3721 acccgtcatc tcatcgtata aatgttggac ttcaccgaca acacccacat tgcgtttgat
     3781 tccaaaataa tcttcctgta atgtccttcc aaagattctc acctggccgt ttgtaggcgg
     3841 cagtaatccc aacatcatcc ggatggtcgt actttttccg gcaccgttgg ggccaaggaa
     3901 accatatatt tctccctttt caacgtgaat ggaaagtccg ttgacgaccc gttcaccgtc
     3961 atattctttc gttaatccat caattttgat catcgggttc tccctctatt gttctgcaaa
     4021 aggttgttta gatctcaatc gccgtatatc tcggattcga tgagaaaaca tcctgagaag
     4081 aaaataaagc tgggttcgaa atgataacat ttctttcaat ttctctgaat gaacgtggtt
     4141 gagaaaaggc gaactgggtt cccctcctcc caaccaacac ttctggaaac ataaatttta
     4201 attactcttg atcaattcca aaaaacttct tgaatttctt cgataacctt tcggaaaacc
     4261 attcaagaaa aaacagacaa tattgaggga attatttttt tgccagcctg aggccttctc
     4321 ttcctaagaa tctggcccga ctccccagtt ctgcctcgat ctttaataaa cggttggctg
     4381 tttcttcaat gccgccctcg cgaagatggc cggtgttaaa gcccacagcg taatcagcga
     4441 tatctacacc ctcgcctcta ctgctgcacg gcatcacccc ataaccgcag cggtaggcgt
     4501 attgaatcat ctccgccgcc tcagtaatcg ttcccttctg attgaccttg agtaaaactg
     4561 cattggccgc acccatcgca gccccttttg agacattttc aatattggag gcaaagagat
     4621 cgtctcctac gatctcgatc cccagttcct tggtgagtct ggcaaatcct tcaaagtcgg
     4681 tttcctccaa tggatcctcg aaaatcacga aagggtattt cttaaccatc tctttataga
     4741 gactgatgag ctcctccttt gtcttgtcct cgggggaaaa tagcccgaca tatttttgtt
     4801 tctcggcatc gtagtaagtg gttgccgcca catcaacctg aatccccatt ttcccttcat
     4861 aaccgagatt ggtgatagtc tcggccatga tctcccaaag ttcccgatcg tgtttcacaa
     4921 taccggcatt gatgataatc cgatcataaa actccaaact atagtggagg ttgagtttac
     4981 gatttaagac attgagatat tcggagaaag ccctccaacc agcgagagaa gcttcataat
     5041 agctgtcaaa cccgtaacac atcagcgaat aagtcggttt acagccggac tttttaccag
     5101 caccgtgccg ccaggctccc aaaaccgtta acacacctgg gaccggaagg acacaagcgt
     5161 taatgcctcc gatatgttgg tagagcggaa ttcccaaact ggcagcaccc gcttttagag
     5221 ccgcagccga aacgctaccg gtagcattag cccccaaatt ggatttgtcg ggcgtaccat
     5281 caagctcaag cagtaattga tcaatctccc attgcttgct tgtatcccgg cccttcaatg
     5341 ccgggccgat gattttattc acattgtcga ccgcgcgttt gactcctttg cccaagtagc
     5401 gttggtcctg atcgtagacg aacttggcct catttcctcc aaacgaagtg cctccggttg
     5461 ccacagccac acccacagat ccgttttccg tgacgatcgt ggcctctact ccaaaatgcc
     5521 tgcgttgcga gtatacctcc cgtgccgtaa ccgaaacaat cttgctgcca gaattcatga
     5581 tgcatcctcc ttatataggc cgtaaagccc ttattaccta ataaatcatc agtccaaaat
     5641 caaagtgacg caaa
//
LOCUS       DASVMB010000181         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_317331,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000181 DASVMB010000000
VERSION     DASVMB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317331"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF531_05425"
     CDS             <1..227
                     /locus_tag="VF531_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713442.1"
                     /translation="SMYAFLTAWNDTISALILTNRNQTLSLVVYKAIGTTSSGIQYAA
                     AGSVILILPALVFTFIVRKYVNQMWGGVEL"
     gene            240..1340
                     /locus_tag="VF531_05430"
     CDS             240..1340
                     /locus_tag="VF531_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713443.1"
                     /translation="MSYLVLKDLEKRYSNSGSLVVNKMNLSIEKGEFIVFLGPSGCGK
                     TTTIRMISGLEEVSGGDILIEGQSILGKKPKDRGVSMIFQSYAIWPHMTVYENIAYPL
                     KLQKLPKSEIKKRVTAAAEATHIETLLDRYPAQMSGGQRQRVAVARAIVVKPKIFLMD
                     EPLSNLDAKLRVSMRTELKNIHNKENSTSIFVTHDQSEAMSLADRIVIMNQGVVEQIG
                     TPMEVYHDSATKFVASFIGTPPTNFFEVRIREIDNQLFAVAKSFQYKIQPELTAALKP
                     YLHKVVDMGIRPEFIDIAAEPENSGYLCETTINFVEPQGTHSILIVNLEGKNVKILTT
                     KYLDIKPDIKVSLHIRNGKAMFFDTKTFCRIK"
     gene            1416..2345
                     /locus_tag="VF531_05435"
     CDS             1416..2345
                     /locus_tag="VF531_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7713444.1"
                     /translation="MTPKERAVAALTRNIPDYVPTFELEFQLEEEMFGKSFLRQKDLE
                     DKTAGEKGILIEKNAEYMVQVYEALEYSIIPVHYLDDDGIEATIKALRKIVGDKYLIT
                     THGDGTYKIPEGAEMLDFVYWLADKPEEAKQVAAKNAEKAIERNKRFINAGIDCFILC
                     RDYCFNSGPFLSPEMFREFIQPYLYRIIKETREAGAYTIKHTDGNIMPILDQLVECNP
                     HALHSLDPMAGVDIREVKHLVGDKVCLCGNVHCAALQTGTDEEVIASAEYCLTYAKPG
                     GGYIFSTSNCVFKGMRPERYQLVQDVWKRMRAY"
     gene            2413..>3683
                     /locus_tag="VF531_05440"
     CDS             2413..>3683
                     /locus_tag="VF531_05440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7713445.1"
                     /translation="MKIALAHFRVGETDGVSLEMDKWAKVLERMGHTVVYISGSKGNV
                     ARDTFVIEEMYYQSEQNARLKAAFYRNLGLYTEASLKNEILQVAKRIETKLAEIIKSQ
                     GIEVIVPNNIFSLGHDIPTAIGFMGAITKTGVKVVFHHHDFWWESSRPYYFNQTTEWG
                     KTVMDTLFVPKIENDNFRHCVINSLAQKGLREKSGVDSVVVPNVFDFGAPAWRQDEYN
                     STFRRELGVPEDDILVLQGTRVTNRKAIELAVDVVAMLNSQRFREKLKNSNKQFTLAI
                     LGLHEGVNGYEKVLFNYIEKKGVRAIINDKIVDHTRSMKQGHKVYSLWDAYVYADIVT
                     YPSIQEGWGNQFLEGIFARVPMVVFEYDVYKADIGTKGFEVFSLGDSYRIRENGLVYV
                     KEEKLAKVCDEIVNTINDDDRREYTADKNYSL"
BASE COUNT         1150 a          659 c          932 g          942 t
ORIGIN      
        1 gttcgatgta tgcgttttta acggcatgga atgataccat atcggccttg atattgacaa
       61 atagaaatca aaccttatca ttggtagttt ataaggcaat cggtacaact tccagcggaa
      121 ttcaatatgc agcggcagga agcgtaatat taattttgcc ggcattggtt tttactttta
      181 ttgtaaggaa atatgtcaac caaatgtggg gcggagtgga attataggag gaatcaatca
      241 tgagctatct tgtactaaaa gatttggaaa aaaggtattc aaattccgga agtctcgtcg
      301 tcaacaaaat gaacttgtcg atcgaaaaag gtgaatttat cgtatttcta gggccgtcag
      361 gttgcgggaa aacaacaacc attcggatga tctccggttt ggaagaagtc agcggcggag
      421 atattctgat tgaaggccaa agtatcctgg gcaaaaaacc gaaagaccgg ggagtatcga
      481 tgatattcca aagttatgcg atatggcccc atatgacagt ctatgagaat attgcctatc
      541 cgctgaaact ccagaaattg ccgaagtctg aaatcaaaaa gcgggtaacg gcagcggctg
      601 aggccaccca tatcgaaacg ttgcttgacc ggtaccctgc tcaaatgtcg ggagggcaaa
      661 ggcaaagagt tgcagtagcc cgcgcaattg ttgtaaaacc aaagatattc ttaatggatg
      721 aaccgttgtc aaaccttgac gccaagttga gggtctccat gcggaccgag ctcaagaata
      781 tccataataa ggagaactcc acctccatat ttgtgaccca tgatcaatcg gaggccatga
      841 gtctggcaga ccggatcgtc atcatgaatc agggcgtcgt tgagcaaatc ggaaccccga
      901 tggaagtata ccacgacagc gctacgaagt ttgtggcctc atttatcggc actccgccga
      961 cgaatttctt tgaagtcagg atccgggaga ttgacaatca attatttgcg gtggcgaaat
     1021 cttttcaata caaaattcaa ccggaattaa ccgcagcttt aaaaccttat ctccataaag
     1081 tggtagatat ggggatcagg ccggagttca ttgatattgc agcggaacca gagaactccg
     1141 gatatttgtg tgagaccacg ataaattttg ttgagcccca agggacgcac tcaattctca
     1201 tcgttaatct tgaaggtaaa aacgtaaaaa tattgaccac caagtatttg gatattaagc
     1261 ccgatattaa agtgtcactc catataagga atggaaaggc aatgttcttc gatacgaaaa
     1321 cattttgcag aataaagtaa aaacaccttt aaggtttatc ggcggagtat tttttggtcc
     1381 tgaaaggctg gaatgataaa cgaaagggag acattatgac accaaaagaa agagccgtag
     1441 ctgcattgac ccgaaatatt cctgattatg tccctacttt tgaactggag tttcaattag
     1501 aggaggaaat gtttggaaag tcgttcttaa ggcaaaaaga cttggaagat aaaactgccg
     1561 gggaaaaagg gatactaatt gaaaaaaacg cagaatacat ggttcaagtt tatgaggctc
     1621 tagagtattc aattatcccc gtacattacc ttgatgatga cgggattgaa gctaccatca
     1681 aggctttgcg aaaaattgtc ggggataaat acttaatcac tacccatggg gacgggactt
     1741 ataaaatacc tgagggcgca gaaatgctcg attttgtcta ttggctggcg gataaaccgg
     1801 aagaggccaa acaagtagcg gcaaagaatg ccgagaaggc aatcgagcgg aataaaaggt
     1861 ttataaatgc cggaatagac tgttttatcc tgtgccggga ttattgtttc aacagtggtc
     1921 cctttctgtc gccggaaatg tttagggaat ttatccagcc atatttatat cggatcatta
     1981 aggagacccg tgaagcggga gcttacacca tcaagcatac cgatggcaac atcatgccca
     2041 tacttgatca attggtggaa tgtaatcccc atgcgttgca ctctttagat ccgatggctg
     2101 gtgtggatat ccgggaagtt aagcatttgg tgggagataa ggtgtgcctg tgcggaaatg
     2161 tacactgtgc ggcgcttcag accggtaccg atgaggaagt cattgccagt gcggaatact
     2221 gtctcaccta cgcgaaaccg gggggaggat atattttcag caccagtaat tgtgtgttta
     2281 agggtatgcg tcccgaaaga tatcaactgg tgcaggacgt ctggaaaagg atgagggctt
     2341 attaactgat agtgaatgga ttcgagcgga gaggatttta aaaaggctgt ttcttctgcg
     2401 ggaggaaatg gaatgaaaat tgcattggcg catttcaggg taggagaaac ggacggagtc
     2461 tcccttgaaa tggataaatg ggctaaagtg cttgaaagaa tgggacatac ggtggtctat
     2521 atttcgggtt caaagggaaa tgtagcccgg gatacctttg taattgaaga gatgtattac
     2581 caatcagagc aaaatgcaag attaaaggcg gcattttacc gcaatttagg tttatacacc
     2641 gaggcaagtt tgaaaaatga aatactgcaa gtagcaaaac gaattgagac caagctggct
     2701 gagattatta agagtcaggg catcgaagtc attgtgccga acaatatttt ttcgctggga
     2761 catgatatcc ctactgcaat aggcttcatg ggcgcgataa caaagacagg ggtaaaggtt
     2821 gttttccacc atcatgattt ctggtgggaa tcctcccgtc cttattactt caatcagacg
     2881 acggaatggg gaaaaaccgt catggacaca ctttttgtgc cgaaaatcga gaatgacaac
     2941 tttagacatt gtgtcatcaa ttcactggcg caaaagggtt tgagggagaa gagcggagtt
     3001 gattcggtgg tggtacctaa tgtctttgac tttggcgcac cggcgtggcg gcaagatgaa
     3061 tataattcta catttcggcg tgaacttgga gttccggaag atgatatcct tgttctccaa
     3121 gggacccggg taacgaacag aaaagcgatt gaactggcag tggatgtggt ggcaatgtta
     3181 aatagccaaa gattccggga aaaattgaaa aatagcaata agcaatttac actcgcgatt
     3241 ttgggattgc atgaaggagt aaacggttat gaaaaagtgt tgtttaacta tattgaaaaa
     3301 aaaggcgtaa gagcaataat aaacgataaa atcgtggacc atacaagatc catgaaacaa
     3361 ggtcataagg tttattccct atgggacgct tatgtttatg cggatattgt cacctatcct
     3421 tcaattcagg aaggttgggg gaaccagttc ctggaaggga tttttgcccg ggttccaatg
     3481 gtcgtgtttg aatacgacgt atataaggcg gatattggta cgaagggatt tgaagtattt
     3541 agcctgggag atagttatag aatcagggaa aacggccttg tttacgtaaa agaagaaaag
     3601 ctggcaaaag tatgcgatga gattgttaat acaatcaatg atgatgacag acgggaatat
     3661 acggctgata agaactatag cct
//
LOCUS       DASVMB010000182         7208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_23897,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000182 DASVMB010000000
VERSION     DASVMB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23897"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            450..1463
                     /locus_tag="VF531_05445"
     CDS             450..1463
                     /locus_tag="VF531_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7713446.1"
                     /translation="MSNIKDVAKTAGVSIAAVSRILNDPSYGADETRAKVMAAIKQLG
                     YRPNNIARSMVKGKTHLIALVIPDVRNPFFTETARGVEDVANKYDYRVMLCNTDEDLA
                     KQQKYLETFRSKIIDGFIIAVASEKDKELGKMNRQEMPFVLIDRQSPGVGADLIVVDN
                     KGGAYEATRHFIRLGHKKIGIITGKLDTLTGRDRLNGYIAALKEAGLTIRQEMIFDGE
                     FTIPGGYNATRVMLNQSEKPTALFISNNSMMIGALKAISEAGIKIPNDLAIIGFDDEE
                     WAQFFMPPLTVVRQPAYTMGTLAAELLFQRLQENGSSERKEIVLKPELVVRESCGGRE
                     NKK"
     gene            1533..3335
                     /locus_tag="VF531_05450"
     CDS             1533..3335
                     /locus_tag="VF531_05450"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEX7713447.1"
                     /translation="MNKTVIRNRLIGTLPKIGVRPIIDGRRRGIRESLEVQTMTMAQN
                     VARLLVRNLRHPNGMPVECVIADTCIGGAAETAQAADKFAREGVGVSISVTPCWCYGA
                     ETMDMDPFTPKAVWGFNGSERPGAVYLASVLAAHNQKGLPAFGIYGQDVQNADDTTIP
                     EDVQEKLLRFAKTGLAVATMRGKSYLAMGGVAMGIAGSMVDPGFLESYLGMRYEMVDL
                     SEFTRRIAEEIYDPDEYRRALQWVRENCKEGVDLNPRDNQFSRAKKDENWEMVVKMSI
                     IARDLMIGNPVLDRLGFGEEALGHNAIAAGFQGQRQWTDHFPNGDFLEAVLNSSFDWN
                     GIREPFVMATENDYLNGISMLFGHLLTGTAQMFSDIRSYWSPEAVKHLTGYKLNGSAK
                     NGFIHLINSGSTALDATGRQTSNGKPIIKPFWEITRDEVRQCLDATEFCPARLEYFRG
                     GGFSSEFVTRGEMPATMFRLNLVKGLGPVLQIAEGYIINLPDDLAKIIINRTDPTWPT
                     TWFVPNLTGADPYKTVYSVMNNWGANHGAISYGHIGADLLTLASLLRIPVSMHNVAEE
                     KIFRPSAWNAFGAMDRQGADYRACSTLGPLYGKN"
     gene            3455..4534
                     /locus_tag="VF531_05455"
     CDS             3455..4534
                     /locus_tag="VF531_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX7713448.1"
                     /translation="MAEYNGLGMGLGDLCRLSPAQSRSISAENFSGAKGRGGMATEGT
                     GAECARDLGRGWKLSPSVKIAAKTVFTMAEITEPGAIKHIWMTCSPGNWRSFIMRIYW
                     DGEINPSVEVPVGDFFCNGWCERSNVSSLPVAVNPAGGMNGYWEMPFRKACKITMENL
                     SDDETVLYYQVDYILTTVPDDVAYFHAQWRRSNPVSGAIHTILSDVKGQGQYVGTYLA
                     WQVNNCGWWGEGEVKFYLDGDRDFPTICGTGTEDYFGGAWNFEQPQGHYGIYSTAFLG
                     MHQTIQPDGLYRSQQRFGMYRWHIMDPVRFGQDLKVTIQTLGWRSGGRYLPTHDDVAS
                     VAYWYQTEPHAAFPTLPSKDGLEVI"
     gene            4752..5615
                     /locus_tag="VF531_05460"
     CDS             4752..5615
                     /locus_tag="VF531_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX7713449.1"
                     /translation="MMAQSVYPEKPNTLLKLFHKDKAIIGVIHSLPIPGSPQYHGEDL
                     NEKAYDYAVSEAEAYVKGGVDGIIVENAWDIPFLKPEDIGIETVAAMSVMVDRVRQAV
                     SVPIGVNCLANGAIQALAVAKATGVSFIRSNQWANAYIANEGFVEGAAAKALRYRHNI
                     GAEDVVVFADVHVKHGSHSIVADRSVEEQTRDVEWFAADVLIATGNRTGHATPLEEVQ
                     KIKSATNLPVIVGSGLSGENVENLFTLADGAIVASSLKQGGVWWNPVNVDSVCRLMEI
                     VNRIRSKAKPC"
     gene            5644..5970
                     /locus_tag="VF531_05465"
     CDS             5644..5970
                     /locus_tag="VF531_05465"
                     /inference="COORDINATES: protein motif:HMM:NF042668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6616 family protein"
                     /protein_id="HEX7713450.1"
                     /translation="MGAQERPLGFFAAWKATDTWFRTSEKEKAQFMKEIEKIFNVAKE
                     KGVKMHGVYDCSWSSEWRYFTFWECPNLEILEETMGKLAAIGDINMYNEQRHFIGRLI
                     PEEVIQ"
     gene            6006..6974
                     /locus_tag="VF531_05470"
     CDS             6006..6974
                     /locus_tag="VF531_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7713451.1"
                     /translation="MSKIDVIGYSSSDVDIVLMVNQLPSYDDKIPGALIGRLPGGTMA
                     NYACAISRLGIKSGWTGSLGDDAEAQIVLKGFQEFGVDTSTIRILKNTATMFTVILVD
                     DTGERAIVVVPTFKEPDKLTAEQQKAIAEARLLYTGPYKEIPFFEAAQWARKHDTLVA
                     IDIERTSHLTAPEIDEILANIDVVILNQAMLPELYGISFKEVSDWTSVGDLLRREVIK
                     HRLLYGAVTLGHLGCILADSKQSCFCPGYRVKVVDTTGAGDCFNSAFTFGVLQSWKLE
                     DIGLFSNAAGAISVDSYGARGHLPKYDEVKKFMAGNQPDGKILFQR"
     gene            6994..>7208
                     /locus_tag="VF531_05475"
     CDS             6994..>7208
                     /locus_tag="VF531_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713452.1"
                     /translation="MKQNNFIKVIIEDLTPESFAPFGQAITIPKSKPTKSGDGWDCWS
                     PVALIENTTPMGIGIVATSPLPLMVTE"
BASE COUNT         1930 a         1503 c         1987 g         1788 t
ORIGIN      
        1 aggcggaaaa tcttgaaacc aagatcactg ccttaacagg aaaggctgag gatctgggta
       61 aagagttcat ccagtccgga cgcaggtccg aaagacgttt tgcctggctc tggtttatca
      121 atttacttat tttgatcctg ctggtatgga tgttctgttt tcgctgaatc gaattgggaa
      181 actcagcccg gtggactacc agtattatca gtaacagtga atattacggg aaatcattgc
      241 gggcggccgg ggtgagaaag ataggtgtaa aaaaaaatga tattttatgt tgcagaagta
      301 aaacgtttta catataatca aggtataagc tgattgaacg tcggaaacga atctgataat
      361 ttatttaccg atcaacgctt tttgtatttg cgggaatgat aatcatttat aaaacatttt
      421 accagtaaca aaataaggag taaccgatag tgtccaatat caaggatgtg gcaaaaacgg
      481 cgggagtctc catcgcagcg gtttccagga tactgaatga tccaagttac ggtgcggatg
      541 agacccgggc taaagtaatg gcagccataa aacaattggg ttatcgtccc aacaatattg
      601 cccggagtat ggtcaagggt aaaacccacc ttatcgccct ggtgattccc gatgtccgga
      661 acccgttctt taccgaaacg gcgcggggag tggaggatgt agccaataaa tacgattacc
      721 gggtcatgct ttgcaacacc gacgaagacc ttgccaagca acaaaaatat ctggaaacat
      781 ttcgcagtaa aatcatagat ggattcatca ttgcggtggc atcagagaag gataaggagt
      841 taggcaagat gaaccggcag gaaatgccct ttgttttgat cgaccggcaa agtccggggg
      901 taggggcgga tctgatagta gttgataata agggtggagc ctacgaggca accaggcatt
      961 ttatcaggct gggccataaa aaaataggaa ttatcaccgg caagctcgat accctgaccg
     1021 gccgggatcg attgaatgga tatatcgccg cattgaaaga agcggggttg accatccgcc
     1081 aagagatgat ttttgatggc gaatttacaa tcccgggcgg ttacaatgct acccgggtga
     1141 tgctgaacca aagcgagaag ccgacggcat tgtttatttc aaataattcc atgatgatcg
     1201 gggcacttaa ggccattagc gaagcaggga tcaaaatccc gaacgatctg gcgattatcg
     1261 gatttgatga tgaagaatgg gcgcaattct ttatgccccc gcttacagtt gtccggcaac
     1321 ccgcctatac gatggggaca cttgccgcgg agcttctgtt ccaacggtta caggagaacg
     1381 gttcgtccga acgtaaggag atcgttctca aacccgaatt ggttgtcagg gaatcctgcg
     1441 gtgggcgaga gaataagaaa tgaaggtatt caatttttcg aacgcaatca atacaacaac
     1501 aggaggaaaa gatgaatact gacaaagatg aaatgaacaa aactgtaata cggaaccggt
     1561 tgattgggac gcttcccaag atcggcgtcc ggcctatcat cgacggccgg agacgtggaa
     1621 ttagggagtc gttggaagta caaacaatga cgatggccca gaatgttgcc cggcttttag
     1681 tccggaactt gcggcatccc aatggcatgc cggttgaatg cgtaattgcc gacacctgta
     1741 tcggaggagc ggcggaaaca gcccaggcgg ctgataaatt tgcccgggaa ggagtcggag
     1801 tatccatatc cgtgaccccg tgttggtgtt acggtgccga aaccatggac atggatccat
     1861 tcactcccaa agcggtctgg ggatttaacg ggtcggagcg gcctggcgcg gtatatcttg
     1921 cttctgtttt ggcggctcat aatcagaagg gtcttccggc attcggtatc tatgggcaag
     1981 atgtacaaaa cgcagatgac acaaccatcc ccgaggatgt ccaggaaaaa ttactccggt
     2041 ttgcgaagac cggtttagct gttgcgacta tgcgcggtaa gtcgtatcta gcgatgggtg
     2101 gcgtggccat gggcatcgcc ggctccatgg tggatccggg ttttctcgaa agctacctgg
     2161 gaatgcgtta tgaaatggtg gatttatccg aatttacccg gcggattgcg gaagagattt
     2221 atgatcccga tgagtatcga cgggccttgc aatgggttcg ggagaactgc aaggaagggg
     2281 tggacctaaa tccacgggat aatcagtttt cacgggcgaa gaaagatgaa aactgggaaa
     2341 tggtagtaaa aatgtccatc atcgcccggg atctgatgat tggcaatccg gtcttggatc
     2401 gacttggctt tggcgaggaa gccttgggtc ataatgcgat tgcagcgggt ttccagggcc
     2461 agcggcaatg gacggatcac ttccccaatg gtgatttttt agaggcggta ttaaattcgt
     2521 cctttgattg gaatggtatt cgtgagccgt ttgtgatggc aaccgaaaat gattacttaa
     2581 atggtatatc catgcttttc ggacatctct taaccggcac ggcacaaatg ttttccgata
     2641 tccggtcgta ttggagcccc gaagcagtaa aacatttaac aggatacaaa ttgaacgggt
     2701 cggcgaaaaa tgggtttatt catttaatca actccggttc taccgcttta gatgccacag
     2761 gccggcaaac cagtaatggg aaaccaatca taaaaccgtt ttgggaaatt acccgggatg
     2821 aagtcaggca gtgccttgac gccacggagt tttgcccggc acgacttgag tacttccggg
     2881 ggggcgggtt ctcttcggaa tttgtcaccc gcggtgagat gccggccacg atgtttcgct
     2941 tgaatctggt gaaaggtctt ggcccggtgc tacagatagc ggaaggatat atcattaacc
     3001 tgccggatga cttggccaaa ataatcatca accgcaccga tcccacctgg cctacgacct
     3061 ggtttgttcc taatctaacc ggcgcagatc cgtataaaac ggtttactcg gtaatgaaca
     3121 attggggagc caatcatggg gccataagtt acgggcatat cggagccgat ctgttgactt
     3181 tggcttcgtt attgagaatt ccggtttcca tgcataatgt ggcggaggaa aaaattttcc
     3241 gtcccagcgc ttggaatgct tttggggcaa tggatcgaca gggcgccgat tatagagctt
     3301 gcagtacact cggtccttta tacggcaaga attaatgatt ttcgtttaga tgaaggcggg
     3361 ataacggcat ccgagttgga agtctgtacg ataacctgga tgcgaagcgg acggcggttg
     3421 aagtttctta ataactggac aggaggattt aaaaatggca gaatacaatg gattgggcat
     3481 gggattaggg gatttgtgcc gcctatcgcc ggcccagtcc cgctcgatta gcgctgaaaa
     3541 ttttagcggt gccaaggggc gaggaggaat ggccactgag ggaacggggg cggaatgcgc
     3601 ccgtgacttg ggacggggat ggaagctctc gcccagcgtt aaaattgccg ctaaaaccgt
     3661 atttaccatg gcagaaataa cggagcccgg ggcgatcaag catatctgga tgacttgttc
     3721 cccggggaac tggcgtagtt tcatcatgcg gatttattgg gacggagaga ttaacccgtc
     3781 ggtcgaagtg ccggtaggcg actttttctg caacggctgg tgtgaacgga gtaacgtcag
     3841 ttctttgcca gtggcggtca atccggcggg agggatgaac ggttattggg agatgccgtt
     3901 tcgcaaggcc tgcaagatta cgatggaaaa cctttccgac gatgaaacgg ttctctatta
     3961 tcaagttgat tatatattga cgaccgtccc cgacgatgtt gcatattttc atgcccagtg
     4021 gcggcggagc aatccggtga gcggcgccat acatacgatt ttgagcgatg tcaagggaca
     4081 ggggcagtat gtcgggacat atctggcctg gcaggtcaat aattgcggct ggtggggtga
     4141 gggcgaggtc aaattctatc tggacgggga tcgggacttt ccgacaatct gcggaaccgg
     4201 caccgaggat tatttcggcg gcgcctggaa ttttgaacaa cctcagggac attacggtat
     4261 atattccacc gcttttctcg gaatgcatca gaccatccaa ccggacggtt tataccgcag
     4321 tcaacaacgc ttcggaatgt atcgttggca tatcatggat ccagtccggt tcggtcagga
     4381 tttaaaagtg accattcaaa cgctgggctg gcgttcaggg gggcgatatc ttcccaccca
     4441 tgatgatgtg gcttcggtgg cctattggta tcagaccgaa ccccacgctg ccttcccaac
     4501 cttaccctcg aaagacggat tggaagtcat ttgagtgttc ggagacggtg atctgatgct
     4561 ccgcttggtt ggtaaaatta gctctgtttt ttagaaatgg tggattaacc acttctgggg
     4621 acagagctaa aaatttttta gataaaaaac aaaatcatag caggagaatc gatactatca
     4681 tcgtaataat attatatagt aatgttacta cattacgatg ttacatccaa aaacttatga
     4741 aacgaggagt aatcatggcg cagagtgttt atccagaaaa accgaatacg cttttgaaat
     4801 tgttccacaa ggataaagcg attattggag tgattcattc attgccgatt cccggatcac
     4861 cgcaatatca tggagaagac ttaaacgaaa aggcatatga ttacgcggtc agtgaggccg
     4921 aggcctatgt caagggcgga gttgacggaa ttattgtaga gaatgcatgg gatattccat
     4981 ttttaaaacc ggaggatatc ggaatcgaga ctgtagcagc catgtcggtg atggtcgatc
     5041 gcgtccgtca agctgtttca gttcctatcg gcgttaactg tctggccaac ggagcgattc
     5101 aagctttagc tgtcgccaaa gccaccggag ttagttttat tcgttccaat caatgggcga
     5161 acgcgtatat cgccaatgag ggatttgtgg agggtgcggc cgccaaagcc ttgaggtacc
     5221 gtcataatat tggcgccgaa gatgttgtcg tttttgcgga tgtccatgtc aaacacggaa
     5281 gccattcaat cgtagccgac cggagcgttg aggaacagac ccgcgatgtt gagtggtttg
     5341 cggctgatgt cctgattgca accggtaacc gcaccggaca tgccacgccg ttagaagagg
     5401 ttcagaagat taaatcagcc acgaatctgc ccgtcattgt tggtagcggt ttaagcggcg
     5461 aaaatgtaga aaacctgttt actttggctg atggcgctat tgtcgcatca tcgttgaagc
     5521 agggcggcgt ttggtggaac ccggttaatg tcgacagtgt ctgccgctta atggagattg
     5581 ttaaccggat tcgttccaaa gcgaagcctt gctaactcat taactaccac ggaggaaatt
     5641 tcaatgggag cgcaagaaag accgttaggt ttttttgccg cctggaaggc gacggataca
     5701 tggtttcgta ccagtgagaa agagaaagcg cagtttatga aggagatcga gaagatcttt
     5761 aacgttgcga aagaaaaagg cgttaagatg cacggagtct acgattgcag ctggtcgtcc
     5821 gaatggcggt actttacctt ttgggaatgc ccgaatttgg aaatactaga ggaaacaatg
     5881 ggaaaactgg cggcaatcgg tgatatcaac atgtataacg aacaacgcca tttcattggc
     5941 cgattgattc cagaggaagt tattcagtga aactggaact gagatttagg aaaggggggg
     6001 tatagatgtc aaaaatcgat gtgattgggt acagctcatc ggatgttgat attgtactta
     6061 tggttaacca gttgccatcc tatgatgata aaattcccgg agcactgatt ggccgtctgc
     6121 ctggtgggac aatggccaac tatgcttgtg ccatcagccg gttgggtata aaatccggat
     6181 ggaccggttc acttggggac gatgccgagg cccaaatcgt gttgaagggg tttcaagagt
     6241 tcggtgtgga tacctcgacc atccggatcc ttaaaaacac agcaaccatg tttacagtta
     6301 ttttggttga tgataccggg gaacgggcga ttgtggtagt acccactttt aaagaaccgg
     6361 acaagctaac tgctgaacag caaaaagcca ttgccgaggc gcgtttgctg tataccggtc
     6421 catataaaga gattcctttt tttgaagcgg cccaatgggc ccggaaacat gatacccttg
     6481 tggcgattga tattgaacgc acctcccatt taaccgctcc ggaaatagat gaaatcttgg
     6541 caaatatcga tgttgtgatc ttaaatcagg ctatgcttcc cgaactttac ggcatttcat
     6601 ttaaggaagt ctcagattgg acttcggtcg gggatttgct ccggagagaa gtaatcaaac
     6661 accggttact ctatggcgca gtaactttag gacacttagg ctgtattttg gcggattcaa
     6721 aacagagctg tttttgtccg gggtaccggg taaaggtggt cgatacaacc ggcgcagggg
     6781 actgctttaa ttcggctttt accttcgggg tattgcaatc ttggaaactg gaggatatcg
     6841 gtttattttc caatgctgcc ggcgctatat cggtagacag ctatggtgcc agagggcatc
     6901 tgccaaaata tgatgaggtt aagaagttta tggccggaaa ccagccagac ggtaaaatct
     6961 tatttcaaag atgagaatcg gggcaaagcg gatatgaaac aaaacaattt tatcaaagta
     7021 atcattgaag atttgacgcc tgaatcattt gcgccgtttg ggcaggcaat taccataccc
     7081 aagagtaaac caaccaaatc gggagatggt tgggactgct ggtcgccggt ggctttgatc
     7141 gaaaacacga ccccgatggg tatcggaatc gtcgcgacca gtccgcttcc tttaatggtg
     7201 acggagat
//
LOCUS       DASVMB010000183        14484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_317554,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000183 DASVMB010000000
VERSION     DASVMB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14484
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317554"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VF531_05480"
     CDS             complement(<1..1480)
                     /locus_tag="VF531_05480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX7713453.1"
                     /translation="MAEVNDGKIIPVVLENEVKQSYLAYAMSVIASRALPDVRDGLKP
                     VHRRILYGMYESGTTPDKPYRKSARIVGDVMGRYHPHGDAAIYDTMVRMAQTFSFRKI
                     LVDGHGNFGSVDGDPPAAMRYTEVRMDKLALEMVADIEKKTVDFRPNFDESLEEPVVL
                     PSRFPNLLVNGSSGIAVGMSTNIPPHNLGETIDGAVLLIDNPDADDKELLRVIKGPDF
                     PTGGIILGRQGIRDAYLTGRGSIKVRSKTDIEEQSNGKHRIIVTEIPYQVNKARLIEE
                     IAQLVHDKTVDGITDLRDESDRTGMRIMIELRRDVNPNVILNQLFKHTAMQQSFGAIM
                     LVLVDNKPKILTLREILKYYLEHQREVVTRRTQFDLAKAEDRAHILEGLRIALDHIDE
                     IINLIRSSQTVEIAREGLMTKFALSEKQAQAILDMTLKRLTGLEREKIESEYAELMKL
                     IKYYRELLADVHKIMMVVKEELLAIKTKYNDPRRTEIAKDEGE"
     gene            complement(1520..2227)
                     /gene="rpiA"
                     /locus_tag="VF531_05485"
     CDS             complement(1520..2227)
                     /gene="rpiA"
                     /locus_tag="VF531_05485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX7713454.1"
                     /translation="MKNSRENQELKKIVGRAAAGLVRDGMTCGIGTGSTVAFFIEDLG
                     RRVKEEGLHLVGVPTSFQSKILCRQFGIDTADAQDFSTLDLVIDGADEVDPELNLIKG
                     GGAAQTIEKIIASMGREFVVIVDQSKTVDRLGSTFPVPIEIIPAALALVSAAVRSLGG
                     EPRLRMGQRKDGPVITDNGQFVLDARFDSSVELRRVDAELHRTPGVVETGLFFDMAAK
                     ALVGSEGKPVRTLVRKH"
     gene            complement(2224..3102)
                     /gene="metF"
                     /locus_tag="VF531_05490"
     CDS             complement(2224..3102)
                     /gene="metF"
                     /locus_tag="VF531_05490"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX7713455.1"
                     /translation="MKIKNLLGGKKPALSFEIFPPKPDYPLDTVYQTITGLRNLKPDF
                     ISVTYGAGGANRSRTIEIAAHIREAFQIESVAHLTCMGHSPAELDEILVQMREHGIEN
                     LLALRGDPPQGEVSYTPPAGNYRYAVELVRQIRKRGDFCIGAAAYPEGHKECRRWQDD
                     WDYLRAKVEAGVDFLTTQLFFDNRIFYNFLDHMERLGVTVPVLPGIMPILNPGQIKRM
                     VYLSEASIPSQLLKLFDKYGNDNLAFEKAGIDYAIRQIDDLLANGVAGIHLYTMNRAA
                     QIQTIVENSQLKEKHH"
     gene            complement(3474..4895)
                     /locus_tag="VF531_05495"
     CDS             complement(3474..4895)
                     /locus_tag="VF531_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7713456.1"
                     /translation="MEFQQIRDLGEESVLCRTRTGSQDDEIVLDKAKFAVMPRDSVRL
                     EMQKEMRAIVQSEKEISHSSLVKYTALEKFQGELYLVREEPVKSGIRQLNPVEVDIAE
                     ACRIMLKALEVSEVYRQAGIFLEGLSLGMIKRDETGEIRIQDPLVVNHFAKFLEPFYR
                     KDPAPEVVMGKPWNERSVIFCWGNLAYQLLTGVDPFAAKTPEERLAKITQIGAVEPKA
                     LNPRISPELNRLIMECLAKNPDTRPSAETIRIRLEQMLETGGWEVSQAEADEFARQAQ
                     NNRKHFERRERFMIWFRKYGVISCGIVAAILIFVLITVATKPKGVLTASTSPDKVIRY
                     YFQSIKDLNVSLFDETVQNKKNSMESLVTNLYVMNRTQQGMTYNMSQEFVKVRIPRMK
                     LVKISQGDQTARYRADFLLVINLSNQIQYQQRSEIYTLQPVHRIWRITAIRTISEKHW
                     SKKIETPEVSPGPVPSASSAPQN"
     gene            5245..5526
                     /locus_tag="VF531_05500"
     CDS             5245..5526
                     /locus_tag="VF531_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7713457.1"
                     /translation="MKDNEHAVVTVTGHDRVGIVAGVAKTLADYHVNIVDISQTILKD
                     IFAMILLVDISAADNDLLELTKALEATGEALGVKIMVQHEDIFRYMHRI"
     gene            5601..6962
                     /locus_tag="VF531_05505"
     CDS             5601..6962
                     /locus_tag="VF531_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEX7713458.1"
                     /translation="MPFTSQEIMETISMVENYHFDIRTVTMGINILDCADPDLSVAAA
                     KATAKIKNRAAKLVETVALMEQTYGIPIINKRIAVTPISLVAEASRATDYFEFALALD
                     KTAAELGIDFIGGYSALVHKGATTGAIHLIDSIPHALAQTKKVCSSVNLATTKAGINM
                     DAVLKMAQVIKETADLTKNENGIGCAKLVVFANAPEDNPFMAGAFHGIGEPDTVINIG
                     ISGPGVVRAAVERIPDCDFRTLAEEIKRVAFKITRAGELIGRKVSERLEVPFGIVDLS
                     LAPTPAVGDSVANILEAMGLERCGGPGTTAALAILNDAVKKGGAMASSAIGGLSGAFI
                     PVSEDAGMIDAVKAGALSIEKLEAMTSVCSVGLDMIAVPGTTSAEVIAGIIADEMAIG
                     MINKKTTAVRIIPVPGKTVGDQVEFGGLLGSAPIMAVKSFSPERFVHRGGHIPAPFQA
                     LTN"
     gene            complement(6975..7718)
                     /locus_tag="VF531_05510"
     CDS             complement(6975..7718)
                     /locus_tag="VF531_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX7713459.1"
                     /translation="MEIDVILSPSCHSGEQAAGRVAVVIDVLRATSTIVTALGNQALG
                     IIPVLDPGEASGLKRETRYEDCLLGGERRGYKIPGFDLGNSPGEYGPSRVAGKRIILS
                     TTNGTKAIRWAAGARVVLAASFLNIRETVRFLHQTCRDLLLVCSGSHGDLSLEDLTCA
                     GMITDLLTEGLVHPCATIRLSDKARIARYTYLKAQETGLEDFIAGTEHGRYLERIGMG
                     EDLRSCSAVDRYPVLAQLVGNEIRDVELV"
     gene            complement(7747..8601)
                     /locus_tag="VF531_05515"
     CDS             complement(7747..8601)
                     /locus_tag="VF531_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX7713460.1"
                     /translation="MAVSFKADSDHNIAKIVLIGVLLVAAVAGLMILVNHYFIQKALV
                     QKEPLTILLLGTGSDSGSGSVSKTLAANDAMVLVFCNPAASRISFLSIPRGKQGISGM
                     SQGSASNPEQTSNEVSQWVGVPINRYLAVDQQGFKELVDVLGGITVDIPVELHYRDRW
                     GRDLYRFASGRQVLDGEKALIYVKLTESSGDELGRVQRQQEFLKAVLKKARNQANILR
                     VAEYNSIIRKYLKTDIKFEEMLKIGNFLKEIDSGDLDAYVLPGRENNGVWEPDRSSLA
                     ELLRKLEP"
     gene            complement(8845..9198)
                     /locus_tag="VF531_05520"
     CDS             complement(8845..9198)
                     /locus_tag="VF531_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713461.1"
                     /translation="MLLERHSPQTLQLLIKKKWLADSIIGLGSGYLTHLTYQTKDVAP
                     EVLEDYHEMRSGEVYGEIIWVGDEVNRRVAEAEVSSINDFGSFIRFDLRLLEVVPFIR
                     DGAVHVRNDYLCYYG"
     gene            complement(9298..10875)
                     /gene="serA"
                     /locus_tag="VF531_05525"
     CDS             complement(9298..10875)
                     /gene="serA"
                     /locus_tag="VF531_05525"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX7713462.1"
                     /translation="MVKILISDPITPAGINLFKEAGFEVEVKTDHTKEELIAKIKNYD
                     ALIIRSQTKVTADVIAAADNLKVIGRAGVGVDNVDVEAATKKGILVINAPDGNTISAA
                     ELTVAMILALARNIPQAHASLKGGAWDRKSFTGTEINGKMLGVVGMGRIGSEVAKRMQ
                     AMGMSILAFDPFLTSEKAESLGVTLADLDTVVTQSDFITVHTPLTAETKGLFGAAEFA
                     RMKKGVRLINCARGGIYDEAALAEALRSGQVAGAALDVFNGEPPTNRELIDLPQTVVT
                     PHLGASTKEAQENVAVDVAVEVIKVLKGETFKNAVNLPPLRSEVRTALQPYFGLMEVL
                     GKFMAQLAAPRINKLEVSYLGEISKYDTSYLSTLFLKGILQASLGEDVNQVNALYLAK
                     ERGLKVNEHKTQDGAGYASLVKVAVTTDQGSHTLSGTVIEKDESRIVELEGYHFDIVP
                     ASNMIVINHSDQPGVIGEVGSILGQNGINIATMQVGRKKQGGTAIMVLNVDSVANGII
                     AKLEKAKTVAKAWGINL"
     gene            complement(11553..12911)
                     /gene="ypeB"
                     /locus_tag="VF531_05530"
     CDS             complement(11553..12911)
                     /gene="ypeB"
                     /locus_tag="VF531_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HEX7713463.1"
                     /translation="MKNKFVIPVLFLALVAVTGWGYSQFQMRRQWEISAENQYQRSYE
                     ELTGHVNNMETAMSKAMVASSFPQSISLLTSVWREANSSQENLGQLPLTSMELNRTKT
                     LLAKVSTFSYNAAQNRLLKGNRPTESEWKNLSGLRDQTRVVSKHLLNLRQQFFNNRAR
                     WLEVDRLGTLGAAGAPASLNSNKVTKAFLMLEDGLRRVPDIQFEGNNLDFVPKPTGLS
                     GSNITARDAVDVARRFLGTEYKTANIQYEQLIKGGFQSYMLRATGPRNTSQDRRLSIS
                     VKGGHVAWMLGNRPVAKSNLSLNQAEERAKAFLTRNGYTNMRGVGRERFANIATISFV
                     PDRNGVLYYPELIKVQVAQDNGEILGFDSTAYLTFHEPQGIPAGSPKINEAQVRKTIN
                     PHLRVERVQMAQVLDEMYNKVLCYEVQGTQGRDRYLTYYNATSGKEEKIRRIDGNGNE
                     VQ"
     gene            complement(13000..13695)
                     /locus_tag="VF531_05535"
     CDS             complement(13000..13695)
                     /locus_tag="VF531_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713464.1"
                     /translation="MFTKIFEKEIKSLFVPAVFVIGLIGCWDLFLGTRIRVWPGQFIG
                     ILAFLPGMIPAIWLLAMGFQSLHAEWNSNSIYLLLSLPVRGWKVTGAKLVADLVSSII
                     IGLVSLAGLGILLTLMGTKLLLHVSAGIIVFMACATLFFMALVIAISQFSFITGHLVP
                     KFRGAFSLVTFFLCLGVYFKLTAWLLPLLNWLPSLEVGRAGLPTGFLLLELIFFGIFF
                     TAGSLLFEKRVEL"
     gene            complement(13695..>14484)
                     /locus_tag="VF531_05540"
     CDS             complement(13695..>14484)
                     /locus_tag="VF531_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713465.1"
                     /translation="FDGMRCAPAGCLPEGTGYSRIEKEMARLIAEQPLLEIRDLKKNY
                     YGTAALKGVSLKIRKGMILGLLGPNGSGKSTLLKAVAGLVRPSSGSIMINGVKPSFQT
                     KADVAYLPEIDHLYAWMTVREIMGFVAAFYPDWEMERAMESLRQLNLNPGQKVGSLSK
                     GQRAQLKLLLALARRVPLILLDEPLSGIDPPSRANIIQMLITGYRDGEQTIICSTHQV
                     GEMEGIFDEVLFLRDGEVALWDSAEKLRQEHGCSIQDLWGRVYH"
BASE COUNT         3501 a         3989 c         3380 g         3614 t
ORIGIN      
        1 actccccttc atccttggca atctcggtcc gccgcggatc gttgtatttc gtcttgatcg
       61 ccagtagctc ttccttgacg accatcatga tcttgtgcac atccgccagc aattcgcgat
      121 agtatttaat gagcttcata agctcggcat actcggactc aatcttctcc cgctccagtc
      181 cggtgaggcg ttttaaggtc atatccaaaa tggcctgggc ctgtttttcg gatagggcga
      241 attttgtcat taacccttcc cgggcgatct ctaccgtttg cgaggagcgg atcaggttga
      301 tgatctcatc gatatgatcc agggcgatcc gtaaaccctc gaggatatga gcccgatcct
      361 ccgccttggc cagatcaaac tgcgtacggc gggtaaccac ttcgcgctga tgctccagat
      421 agtattttag aatttcccgg agcgttaaga tcttgggttt gttatccacc aacaccagca
      481 tgatggcgcc gaaactctgc tgcatcgcag tatgtttgaa tagttggttc aagattacat
      541 tgggattgac gtcacggcgc agttcgatca tgatccgcat cccggtacgg tcggactcat
      601 cacgcaaatc ggtgatgccg tcgacagtct tgtcatggac cagttgcgcg atttcctcga
      661 tcaaacgggc cttattcacc tgataaggga tctcagtgac gataatccgg tgctttccgt
      721 tactctgctc ctcaatatcc gtcttagaac ggaccttgat cgaacccctg cccgtcagat
      781 acgcatcgcg aatcccctgc cggcccagaa taatgcctcc ggtcggaaaa tcgggaccct
      841 taatcacccg caacaattcc ttatcgtccg cgtcgggatt atcaatcaac agtaccgcac
      901 cgtcaatcgt ctctcccaga ttgtgggggg gaatattggt ggacataccg accgcaattc
      961 cggaggaacc gttgaccagg agattcggaa aacgggacgg caacaccacc ggttcttcaa
     1021 gactctcatc gaagttgggc cggaaatcga cggtcttttt ttcaatatcc gccaccatct
     1081 ccagggccag cttatccata cggacctcgg tataccgcat ggcggccggg ggatccccgt
     1141 caaccgaacc gaagttaccg tgaccgtcga ccaatatctt ccggaaggaa aaagtttggg
     1201 ccatccggac catggtatca tatatcgccg cgtcgccgtg aggatgataa cgccccatga
     1261 cgtcacccac gatacgggcc gatttacggt acggcttatc gggagttgtg cctgattcgt
     1321 acatgccgta aagaattcgc cggtgtaccg gtttcaaccc gtctcgtaca tcgggtaaag
     1381 cacgactggc aatgacgctc atggcgtatg ccaggtacga ttgttttacc tcattctcga
     1441 gtactaccgg aataatcttg ccgtcgttca cttctgccat cctcgttgcc caccttatat
     1501 aaaatcagta attgattaat caatgtttcc gaaccaaagt ccgcactggt tttccctccg
     1561 aacccaccag cgccttggcg gccatatcaa agaaaaggcc ggtttcaacc actcccggag
     1621 tccgatgcaa ctcggcatcg acccggcgta attccaccga agagtcaaat ctggcatcca
     1681 aaacaaactg gccgttgtcg gtgatcaccg gcccatcctt tctttgaccc atccgtaacc
     1741 ggggttctcc tcccaggctc cggacggcgg cgctgaccag agccagggct gccgggatga
     1801 tctcaatcgg caccggaaag gtgctcccca gccggtcgac ggttttggat tgatcgacga
     1861 tcaccacgaa ttcccggccc atcgaggcaa tgatcttctc aatggtttgg gcggccccgc
     1921 cgcctttgat cagattcaac tccggatcta cctcgtctgc gccgtcaatc accagatcca
     1981 aagtagagaa gtcctgggca tcggcggtgt cgattccaaa ctgccggcac aaaatcttgg
     2041 attgaaacga agtaggtacc cccaccagat gcaacccctc ttccttgact ctacgcccca
     2101 gatcttcaat gaaaaaggcc accgtggaac cggtgccgat accgcaggtc atgccatcgc
     2161 ggaccaaccc cgccgcggcc cgaccaacta ttttttttag ctcttggttt tctcggctgt
     2221 tcttcaatga tgtttctcct tcagttgcga gttctcaacg attgtttgga tctgggctgc
     2281 ccggttcatg gtatacagat ggattcctgc tactccgttg gcgagtagat cgtcaatctg
     2341 gcgaatggca taatcgatac cggccttttc gaacgcaaga ttatcattcc catatttatc
     2401 aaaaagcttt aataactggg aaggaattga ggcctccgaa agataaacca tccgtttgat
     2461 ctgaccggga ttcaggatgg gcatgattcc cggtaaaacc ggaaccgtta ccccgagcct
     2521 ttccatatga tcgaggaagt tataaaagat tcggttgtca aagaagagct gggttgttaa
     2581 gaaatcaacc cctgcctcca ctttggcccg gaggtaatcc cagtcatcct gccagcggcg
     2641 acattcttta tgcccctccg gatatgccgc ggctccgata caaaaatcgc cccgcttgcg
     2701 gatctggcgt accaattcaa ctgcataacg gtaattaccg gcaggcggcg tgtaagagac
     2761 ttctccttgc ggcggatcgc cccgcagcgc caaaaggttt tcaatcccat gttcccgcat
     2821 ttgaacgagg atttcatcca gctccgcagg tgaatgcccc atgcaggtaa gatgggccac
     2881 tgattcgatc tggaacgctt cccggatatg ggctgcaatc tcaatggtcc gggaacgatt
     2941 agcccccccg gcaccataag ttacgctgat aaagtcgggt ttgagattac gtaggcctgt
     3001 gatagtctga tagactgtat ccagaggata atcaggtttg ggcggaaaga tctcaaatga
     3061 aagtgccggc ttttttcccc ccaaaagatt cttgatcttc atagttaacc cctttataac
     3121 atcacaattt caatgaatct ccaaaaggtg ctacttaatc attcatcaca tggaagtttt
     3181 gatagtgatt tatatgacga attactagtt ttcgccattc atttgccaaa tccttttcca
     3241 atccccgaac gttgaagtat tttggatccc cgccatcttc aattgaaaca ttaaaaatac
     3301 cggaagcccc ctgcttttcc gtataagtaa aaagtcctta ccccggtcgg gcaggacctt
     3361 tcgctttgac aagagcattc ctgatgttac aaattctgct tcctcacacg aatagttttt
     3421 ttgttccctt tagtattcca tgttgcacat ttaccccgta tgttcgggaa aaatcagttc
     3481 tgcggggctg atgatgcgga tggtaccgga ccgggtgaaa cctccggggt ctcgatcttc
     3541 ttggaccaat gcttctcgga gatggtccgg attgcagtga tccgccagat acgatgaact
     3601 ggctgcaggg tgtaaatctc acttcgttgc tggtactgga tctgattgga caggttgatc
     3661 acaagcagga aatcggctcg atatcgcgca gtctgatccc cttgagaaat cttgaccagc
     3721 ttcatccggg gaatccggac cttcacaaac tcctgggaca tgttataagt cattccctgt
     3781 tgggtccggt tcatcacata caggttggtg accaaagatt ccatgctgtt cttcttgttt
     3841 tgcaccgtct catcaaaaag cgagacattt agatccttga tgctttgaaa gtagtagcgg
     3901 attaccttat ccgggctggt ggacgcggtc agaactccct tcggtttggt ggcaacggtt
     3961 atcaaaacaa agatcaatat ggcagcgaca atcccgcagc taatgacccc gtacttccgg
     4021 aaccagatca tgaaccgttc acgccgttcg aaatgtttcc ggttattctg cgcctgccgg
     4081 gcgaattcat cagcttcggc ctgggagact tcccatcctc ccgtttccag catctgctcc
     4141 aaccgaatcc ggatcgtctc cgccgatgga cgggtatccg ggtttttagc caggcactcc
     4201 atgatgaggc ggtttaattc cggacttatc cgtggattaa gagccttggg ttcaaccgct
     4261 ccgatctgtg tgattttggc cagccgttct tcgggcgttt ttgccgcgaa aggatcgacg
     4321 ccggtcaata attgataggc cagattgccc cagcaaaaga tgaccgatct ttcattccag
     4381 ggtttaccca tgacgacctc tggagccgga tccttccggt aaaacggttc gagaaattta
     4441 gcgaaatgat tcacgactaa cggatcctga atccggatct ctccggtttc atctcgcttg
     4501 atcatcccca gactcaggcc ctccaagaag atgccggcct ggcgatatac ttcgctgact
     4561 tccaatgcct ttaacataat gcgacaagcc tcggcgatgt ctacctccac cggattaagt
     4621 tgccggattc cggacttaac cggctcttcc cgcaccagat aaagctctcc ctggaacttt
     4681 tcgagggcag tgtatttcac gagcgaacta tgactgatct ctttttcact ctgaactatg
     4741 gcgcgcatct ctttctgcat ttccaggcga acactatcgc gcggcatgac cgcaaactta
     4801 gccttgtcca ggacgatttc gtcgtcctgt gaaccggtgc gcgtgcggca caatacggat
     4861 tcctctccca aatcccggat ctgttgaaat tccatgaaca tccctccgaa aactcgctgt
     4921 aattctcata ggggctgcag attaagaaaa aagaggtctt tatctatacc aaactgccgt
     4981 ggtttatgat gaacggttcc gatttgataa tttttcccgc tcacagcttc ttgtagtttg
     5041 attatactat attaacgaat caatgataaa aaaatttcaa attcgaagtt tcccgtcggt
     5101 cattttactt taaaaaatag taattcccca cgagcggtgc aggagtaccc tgagcaatcg
     5161 cgaattatct attacccggt ggctgtatag gtaaggtcat gatagtatat tgccgccggt
     5221 gcaatagtta ctgaggtgat ttcggtgaaa gacaacgaac atgcggtagt aacggtaacc
     5281 gggcatgatc gggtagggat cgtggcgggg gtcgccaaga ccttggcgga ttatcacgta
     5341 aatatcgtgg atatcagcca gacaatactt aaggatattt tcgccatgat cttactggtg
     5401 gatatttctg cggccgataa tgacctgctg gagctgacca aggctctgga ggcaaccggg
     5461 gaagctttgg gggtaaagat catggttcag catgaggata ttttccgtta tatgcatagg
     5521 atctgaaatt tccgtttccc caaaaatccg gcgttcaatg ataaccggat acatcatcta
     5581 ttggttgagg agtgacgaat atgcccttta cttcccagga gattatggaa accatctcca
     5641 tggtggagaa ttatcatttt gacattcgta ctgtgaccat gggtatcaat attctcgact
     5701 gcgccgatcc ggatctgagt gtagccgccg ccaaagcgac agctaaaatc aaaaaccgcg
     5761 ccgcaaaact ggtcgagaca gtggccctca tggaacagac ttacgggata cccattatca
     5821 ataagcggat agcagtgact cccatctccc tggttgccga agcctcgaga gccacagact
     5881 attttgagtt tgccctggcc ttagacaaaa ctgccgccga actcgggatc gatttcatcg
     5941 gcggatactc ggccttggtt cacaagggtg ccactaccgg agccatccat ttgatcgatt
     6001 ccatccctca cgccctggcg cagacgaaaa aagtttgctc ctcggtcaat ctggctacca
     6061 ctaaagccgg gatcaatatg gacgccgtac tcaaaatggc acaggtgatc aaggagaccg
     6121 cagacctcac caagaatgag aacggtatcg gctgcgccaa attggtggtt ttcgccaatg
     6181 ccccggagga taacccgttt atggctggtg cctttcatgg tatcggggag cccgacacag
     6241 tgattaacat cgggattagc ggtcccggag tggtcagggc ggcggtggaa cgcattccgg
     6301 actgtgactt ccggacttta gccgaggaga tcaagcgggt tgcctttaag attacccggg
     6361 ccggtgaact tatcggccgc aaagtatcgg aacgcttgga agtccccttt ggaatcgtcg
     6421 atctttctct tgcccccacc ccggcagtgg gagacagtgt ggccaatatc ctggaagcga
     6481 tgggcttgga acgttgcggt ggcccaggta ctaccgcagc gctggccatc ctgaacgatg
     6541 cggtcaaaaa gggcggcgcc atggcctcat cagccattgg tgggctaagt ggtgctttta
     6601 tccctgtcag tgaggatgcc ggaatgatag atgcggtaaa ggccggggcg ctctccatcg
     6661 agaaattgga ggcaatgacc tctgtctgct cggtaggact cgatatgatc gctgtaccgg
     6721 gaactacctc ggccgaggtg attgccggga tcatcgccga tgaaatggcg atcgggatga
     6781 tcaataaaaa aaccaccgcg gtccgaatca tcccggtccc cggtaaaacc gtcggggatc
     6841 aagtcgaatt tggtggcctc ttgggttcgg ccccaatcat ggctgtaaaa agtttcagtc
     6901 ccgaacgttt cgttcaccgg ggaggccata tcccagcacc cttccaggca ctaaccaatt
     6961 agcccacccc ggagttatac aagctcgacg tcacgaattt cgttccccac caattgcgct
     7021 aagaccggat atcgatccac cgcgctgcag gaccggagat cctcccccat cccgattcgc
     7081 tccagatacc ggccatgttc agttccggca ataaaatcct ccagacccgt ctcttgggcc
     7141 ttgaggtaag tatatcgtgc aattcgggcc ttgtcgctca acctaatcgt cgcacaggga
     7201 tgcaccaacc cttcggtcaa cagatccgta atcattccgg cgcatgtcaa atcctcgaga
     7261 cttaaatccc cgtgactacc ggaacaaacc aacagcagat cacggcaggt ctgatgtaaa
     7321 aaccgtaccg tttcgcgaat atttaaaaac gaagccgcca ataccacccg tgctcctgct
     7381 gcccagcgga ttgccttggt accgttggtt gtggagagga taatccgttt cccagccacc
     7441 cgtgatggtc catactcacc cggtgaatta cccagatcaa atccggggat cttatatccg
     7501 cgccgttccc cgccgagcaa acagtcctcg tagcgagtct cccgctttag ccccgatgct
     7561 tccccgggat ccaataccgg gataattccc agcgcctggt tcccaagggc agtaacgatg
     7621 gtgctggtag cacgtaatac atcaatcacc actgctaccc gtcctgccgc ttgttctccc
     7681 gaatgacacg aggggcttaa aataacgtct atctccaatt ttaaaccact ttccaatcca
     7741 aaattgtcac ggctccaatt tacgcagcaa ttccgcaagg gaactgcggt ccggttccca
     7801 aaccccatta ttctcccgcc ccggcaagac ataggcgtcg agatccccgg agtctatttc
     7861 cttcaagaag ttcccaatct taagcatctc ttcgaacttg atatcggtct tcaagtattt
     7921 gcggatgatt gaattatatt cggctacccg taaaatattt gcctgattac gagctttttt
     7981 caataccgcc tttagaaact cttgctgtct ctggaccctt cccagctcgt cgccgctgga
     8041 ctccgtcagt ttgacataga tcagagcctt ttcaccatcg agcacctgcc gtccggatgc
     8101 aaaccggtat agatcccggc cccatctatc ccggtagtga agttcgaccg gaatgtcgac
     8161 cgtgattcca cccagtacat ccaccaactc cttgaatcct tgctgatcaa ccgcgagata
     8221 acggttgatg gggactccca cccactggga tacctcgttg ctggtttgtt cgggatttga
     8281 tgcggagcct tgactcatcc cggaaatacc ctgtttaccc cgggggattg aaagaaacga
     8341 aatccgggaa gctgccgggt tacaaaagac aaggaccatc gcatcattcg ctgccaaagt
     8401 tttggaaaca ctaccactgc cggagtcact acccgttccc agcagcagaa tggttaacgg
     8461 ttctttttgg accaacgctt tctgaatgaa gtaatgattg acgaggatca tcaaccccgc
     8521 gaccgccgct accagaagta ctccaatgag gacgatcttg gcgatattat gatctgaatc
     8581 ggccttgaaa gagactgcca taaaatcccc tcccgataat taatcaagcc ggtgtgaact
     8641 atttgcctta caaagcacgc gcacgtgctt caccggttgc ctgatctgct tgtaccatta
     8701 taaaacccga aaattaccat caatatggtt atcggtaata ccgcaccaaa tccccagggt
     8761 aatttatact ctcccggcca tccgttttaa aacgaaggag aacatgatga tgtataaaaa
     8821 aaagaatcag gccgtaaccc gattctaccc gtaataacac aaataatcgt ttcggacatg
     8881 gaccgcgccg tcgcggataa acggaactac ttccagaaga cgcagatcaa agcggataaa
     8941 agacccgaaa tcattgatac tggaaacctc cgcctctgca acccggcggt tgacctcatc
     9001 cccgacccag atgatttccc cgtaaacctc tcccgatctc atttcatggt agtcttctaa
     9061 cacttccgga gctacatctt ttgtttgata ggtcaagtgc gttaagtaac cactgccaag
     9121 accgataatg ctgtccgcca accatttttt cttgatcagc aattggaggg tttgcggtga
     9181 atgtcgctcc agcaacaacg taacccctcc tcttaaggtt cgctcctgtt ataatatgtc
     9241 gttcggttcg aggaggtgtt taactgttga acgattcctg ttggtcgcga tggacggcta
     9301 gagattaatc ccccatgcct tggctactgt cttggccttt tccagtttgg cgatgattcc
     9361 attggcaacc gagtcgacat tcaagaccat aatggccgta ccaccctgct tttttcggcc
     9421 aacctgcata gtggcgatat tgatcccgtt ctgccctaaa atggaaccga cctcaccgat
     9481 aactcccggt tgatccgagt ggttgataac gatcatatta ctcgcgggta caatatcaaa
     9541 atgataaccc tccaattcca caatccggct ttcgtctttc tcaatcacag ttcccgatag
     9601 ggtatgggaa ccctgatcgg tggtcaccgc taccttaacc agggaagcgt acccggcccc
     9661 gtcctgagtc ttatgttcat tcaccttgag cccgcgttct ttggcaagat acagcgcgtt
     9721 aacctgattg acgtcttctc ccagacttgc ctggagaatt cctttcaaaa acaaagtgga
     9781 gagataggag gtatcgtact tggatatttc tcccaagtaa ctgacttcca gtttattgat
     9841 ccggggcgcg gccaattgcg ccataaattt ccccaatacc tccataaggc cgaaataggg
     9901 ctgaagtgcc gtccgaacct cggaacggag cggaggtaga ttgacggcat tcttaaatgt
     9961 ttctccctta agtactttga tcacctcaac cgcgacatcc accgccacat tctcctgcgc
    10021 ttccttggtg gatgctccaa gatgcggtgt gaccacggtt tgcggtaaat caatcagttc
    10081 ccggtttgtt gggggttcac cattaaaaac atccagcgcc gccccggcca cctgaccact
    10141 acggagggcc tccgccaaag ccgcttcatc atagattccc ccacgggcac agttgatcaa
    10201 ccggaccccc ttcttcatcc gggcaaactc agcggcgccg aaaagtccct tggtttcagc
    10261 tgtcaatggt gtatgtaccg tgataaaatc ggactgagta actaccgtat ccaaatcggc
    10321 caacgttacc ccgagggact cggccttctc cgaggttaag aagggatcaa aagccaggat
    10381 gctcataccc atggcttgca tcctttttgc cacttcactg ccgatccggc ccatccccac
    10441 aacacccagc atcttaccgt tgatctctgt tccggtaaag cttttccggt cccatgcgcc
    10501 accttttaat gaggcgtggg cctggggaat atttctggcc agggctaaaa tcatggccac
    10561 agtgagttcg gcggcagaaa tggtattccc gtccggggcg ttgataacca ggatcccctt
    10621 tttggtggca gcctccacat caacattgtc cacgcccact ccggcgcggc cgattacctt
    10681 gagattatcg gctgcagcga tgacatcggc ggttaccttg gtttgactac ggatgattaa
    10741 tgcatcgtaa ttcttgatct tggcgattaa ctcttctttg gtgtgatccg tcttgacttc
    10801 cacctcgaac ccggcctctt taaataaatt gatccctgcc ggagtaatcg gatcggaaat
    10861 aagaatttta accatggata aacacctctc cttttataat tattcgtcaa aagtcgaaag
    10921 ttaatttagg cgtgggcatg aggcatgagt attgtaagtt atttattata ggtcataagt
    10981 atgaacgctg aaagtcaatc tattagcgac tcaccccgcg ttatttacac actttcagta
    11041 tggccctaca ccgcgtttta acgcgacctc tctttcggaa aagagcgggg tcgagggggt
    11101 gagttgccgt gtgtctgtta ccgcgcgctc cacggagcgc gatgcctaaa aataaaacgg
    11161 cctttcgtcg caagggacga aaggccgtgg taccacccta atttccaaag gtccgatgaa
    11221 tatcggaact caaggatctt tcggcgttaa cggagctaaa ccgatgggac ttactcttca
    11281 cgccaaaacg gcgttaattt tttcccatga ctcggaggag gattcacccg acgcgtactg
    11341 gcttgcagcg accgccagtt ctctgcgaaa cgcaattcga ggctactttt tccccttcat
    11401 cgccaatgca tatttattta ttggttgtat tatatatgcc caccccagtt tttgtcaaga
    11461 cttccgtttg acttttccat tgcttttccg ttacggattt tccggctaaa gccgcttcct
    11521 gcttaacctg cttaagcagc ccggttctga aattactgga cctcatttcc attgccgtca
    11581 atccgccgga tcttctcttc ttttccggaa gtagcattat aataggtcaa ataccgatcc
    11641 cggccctgag tcccctggac ttcataacat aaaaccttgt tgtacatctc atccaacacc
    11701 tgggccattt gcacccgttc cacccggagg tgaggattga ttgtctttct tacctgagct
    11761 tcattaatct tgggcgatcc ggccggtatc ccttgcggtt catggaaggt taggtaggcg
    11821 gtagagtcga accccaaaat ctccccgtta tcctgtgcca cctgaacctt gatcagctcc
    11881 ggatagtaca gaactccgtt gcgatcgggc acaaaactga tagtggcgat attggcgaac
    11941 cgttcccggc ctaccccgcg catattcgta taaccgttcc gggtcagaaa tgccttggcc
    12001 cgctcttctg cctggtttaa actcaggttg gatttggcca ccggccggtt tcccagcatc
    12061 caggcgacat gaccgccttt tacactgatg cttaggcgtc ggtcctggct ggtgttccgg
    12121 ggcccggtag cccggagcat gtaactctga aaaccgcctt tgatcaattg ctcatattgg
    12181 atattggcag ttttatattc ggtcccgaga aatctccggg ccacatccac cgcgtcacgg
    12241 gccgtgatat tactaccgga aagtccggta ggtttgggga cgaaatcaag attattccct
    12301 tcaaactgaa tgtcgggaac ccggcgtaac ccatcctcaa gcattaagaa agccttggtg
    12361 actttattcg aattcaggct ggcgggggct ccggcagctc ccagcgtccc caaccggtca
    12421 acctccaacc aacgggcacg gttattgaag aactgttgtc gcagattcaa aaggtgttta
    12481 ctcaccaccc gggtctgatc ccggaggccg cttaggtttt tccactcgct ttcggtcggg
    12541 cggttgccct tgagtaaacg attctgcgcc gcattgtaac taaaagtgct cactttggcc
    12601 aagagcgtct tggtccggtt tagttccatg gaggtcagag ggagctgccc caggttctcc
    12661 tgggaggagt tggcctcccg ccagacactg gtcaataagc tgatcgactg cggaaaggaa
    12721 ctggcgacca tcgccttgga catggctgtt tccatattat tgacatggcc cgtcaactcc
    12781 tcgtaggacc tttgatattg attttcggca ctgatctccc actggcggcg catttgaaac
    12841 tgggaatatc cccaacccgt gacggctacc agcgccagaa agagtacggg tataacaaat
    12901 ttgtttttca tcggttgact ccctttctca aggataactt caactagtac tattcccaga
    12961 gaaaggagca gagtatgcat ggaacgggag aaagacggcc tataattcaa ccctcttttc
    13021 aaataagaga cttccggccg taaaaaagat accgaagaaa attaattcaa gaagcaaaaa
    13081 gcccgtgggc agtcccgccc taccgacctc caatgagggt aaccaattga gcagcggcaa
    13141 aagccatgca gtcaacttga aatatacccc taaacacaag aagaatgtta ccaggctgaa
    13201 tgcgccccga aatttcggga ccagatgccc ggtgataaag gaaaactggc taattgcgat
    13261 cacaagcgcc atgaaaaata atgtcgcgca ggccataaag acaatgattc cggcggacac
    13321 atgcaataaa agtttggttc ccataagggt taggagtatt cccaaccccg ccagagaaac
    13381 caagccgatg attatcgaac tgaccaaatc tgcaacaagt ttggctccgg ttaccttcca
    13441 cccccgtacc ggcaaagaaa gcagcaggta gatagagtta ctgttccatt cggcatgtag
    13501 actctgaaat cccatggcca aaagccagat ggcaggaatc attcccggta aaaaggcaag
    13561 tatcccgatg aactggcccg gccaaaccct aatcctggtt ccgaggaaca gatcccagca
    13621 tccgatgagc ccaataacaa atactgcagg cacgaataat gactttattt ccttttcaaa
    13681 gatcttagta aacattaatg atacaccctc ccccataaat cctgaatcga gcagccatgc
    13741 tcctggcgga gtttctccgc actgtcccaa agggcaacct ccccatcacg caggaacagg
    13801 acttcatcaa agatcccttc catttcaccc acttgatgag tagaacagat gatggtctgc
    13861 tccccgtccc ggtagccggt gatcaacatc tgaatgatgt ttgcccggga aggagggtcg
    13921 attccggaga gcggttcatc aagtaggatc aacggtaccc tcctggcgag cgccagcagc
    13981 agctttagtt gggcccgttg ccctttggag agagacccga ccttctgtcc aggatttaaa
    14041 tttaattgcc gcaatgattc catggcacgc tccatttccc aatcgggata gaaagcggcc
    14101 acgaaaccca taatctcccg caccgtcatc caggcgtaga gatgatcgat ctccggaagg
    14161 taagcaacat ccgccttggt ttgaaatgaa ggcttcaccc cattaatcat aatacttcct
    14221 gaactgggtc gcaccagacc ggctacggcc ttaagcagcg tcgacttgcc gctgccgttc
    14281 ggtcccagaa gccccaggat catgcccttc ctgatcttta aactaactcc ttttaacgcc
    14341 gcagtaccgt aataattctt ctttaaatca cggatctcaa gtaatggttg ttccgcaatc
    14401 aatcttgcca tctccttctc aattcttgaa taaccagtgc cttccggcag gcatcccgcc
    14461 ggcgcgcacc tcattccatc gaaa
//
LOCUS       DASVMB010000184         4510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_23956,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000184 DASVMB010000000
VERSION     DASVMB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23956"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF531_05545"
     CDS             <1..380
                     /locus_tag="VF531_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7713466.1"
                     /translation="ELERVKDPFNVNSLALAGGIAALGSKKHIALSKDLVQMGRRQFY
                     EELDELKVDYLPTQANFILIDCKRDTQELYQFLLQNGIIIRPTHSFGLPNFIRVTFGT
                     AEQNNRFFKVFREALKKLEYLAS"
     gene            699..1802
                     /locus_tag="VF531_05550"
     CDS             699..1802
                     /locus_tag="VF531_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7713467.1"
                     /translation="MTDPRLKKLAELLVHYSAKVKPDDFVVVQAEGAATEWIVAVAAE
                     AVRAGAHVETMVDLPEFKEHLLKEGTEAQLKQELQLQKYLVKHADVWLTAWGTQNTKA
                     NSNISAKRLQLAAKGASSWRKIYSERMGNGALRWCGTQYPTQADAQEAEMSLSEYQDF
                     VYGAGLLDTADPIAAWKDIRHEQERWVDYLNTKKELRIVSEHTDIRVGIVGRKWINCS
                     GEVNFPDGEIFTSPLENQIDGYITFSFPGIYMGKQIEGVYLEVKDGKVITASAQKGEE
                     LLKTLLDTDTGARSFGEVAIGTNYGIQKFTRNMLFDEKIGGTVHMAVGDSLAEAGGKN
                     RSAIHWDMLCDMRESGKIYADGELFYEKGKFLE"
     gene            1881..3164
                     /locus_tag="VF531_05555"
     CDS             1881..3164
                     /locus_tag="VF531_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713468.1"
                     /translation="MSSNVVEWIREFLIGYQEKWWELSTDPPELSLYSIREQRTLERE
                     LNQFTRQFPEGLLRLLEQAGSDSVYLGEAGNRLREFTCRVLPGASHLWESSTAGIPFF
                     DAATSAAQVFIQAARDFDPDIAPADIFQALRNLWTANGLQLWAGYPVASTQPLLAYSL
                     LYPYTDNYLDDSRIPLGIKQEFNRRFQDFLDGGEFVPKNTHEASLSRLIGMIRDAYPV
                     PEYPQVAQGLNGIHAAQVRSIKQLFPLPGPYHTDILGISFEKGGCSVLADGFLIHGSL
                     NRDEAQLLFGLGILLQLIDDLRDFEIDRRHGHMTIFTQTAAGGWPIDTITNRLFALAD
                     HLADQAGTFNLPGAAIFEDLFQKGCRFLILEAIARHTRIFTSAYRNYFTSRSPVRLSY
                     LSNLKPRLKRQFHSINHNETIPMEMMIRMTVALGR"
     gene            3242..3796
                     /locus_tag="VF531_05560"
     CDS             3242..3796
                     /locus_tag="VF531_05560"
                     /inference="COORDINATES: protein motif:HMM:NF020527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BofC C-terminal domain-containing protein"
                     /protein_id="HEX7713469.1"
                     /translation="MRFRNRAYVSLLRIITISLIIILTVWGTFRSPEMIRILKQIAAA
                     PSVLFTKPVYRVRLDFQYRECDHHRFTEASFASLNQLKTRLQKYGHYQLQKQSDRHYI
                     CRIGTEGFCASCRDYQFLGISGSNVAVFRGTPRKPGPVKEITVLKAEQLPATERQDLS
                     KGITFRDDKEKLQLLEGLNGLISA"
BASE COUNT         1165 a         1008 c         1147 g         1190 t
ORIGIN      
        1 cggagcttga gcgggtgaaa gatcccttta acgtgaattc cctggcttta gctggcggaa
       61 ttgccgctct cggcagtaaa aagcatatcg ctcttagcaa agatttggta cagatggggc
      121 gacgtcaatt ttacgaggaa ctcgatgaat taaaagtaga ttatctgccg acacaggcga
      181 attttattct gatcgattgc aagcgggaca cgcaggaact ctatcaattt ttacttcaaa
      241 acgggatcat tattcgcccc acccattctt ttggactgcc caattttatc cgcgttacct
      301 ttggcactgc cgaacagaat aaccgtttct tcaaggtatt ccgggaagct cttaaaaagc
      361 ttgagtatct ggcatcgtaa gatcacatgc tgattgctac tttttcaatg atttggattt
      421 gggctggtca tactaacgta tacgcgtaaa accagtcctt ttttattttg taaaaaatac
      481 ttcaaaactg catctctcat tcgcttggcg aagattaaag atttccttct ctcggtgaga
      541 ggagtccggg aatgaatagc caattaattc tggatgaacc ggttttaccc ttaaattacg
      601 gtcctaaatt tgcgtctgtc tcttcatttg gactatacgt ttaagaatgg agaaatgaat
      661 tatatctttg cgactttatt ttgaaagggg taatctaggt gacggatcct cgattgaaaa
      721 aattggccga gcttttagtt cattattcgg cgaaggttaa gccggatgat tttgtggttg
      781 tccaggcgga gggggcggca accgagtgga tcgttgctgt cgccgccgag gctgtccggg
      841 ccggtgccca tgtcgagaca atggtggatc tccctgagtt taaggaacat ctcttgaaag
      901 aaggaaccga agcacaactt aagcaagaac tacagctgca aaaatatttg gtgaaacatg
      961 ctgatgtttg gcttaccgcc tggggaactc agaacaccaa ggccaactcg aacatctccg
     1021 ccaaacgctt gcaattggcg gccaagggtg cgtcaagctg gcggaagatc tattcggaac
     1081 gaatgggaaa cggggcgctc cggtggtgtg gcacccagta tcctactcag gccgatgcgc
     1141 aggaagcgga gatgagtctc agtgaatatc aggactttgt atacggggcc ggtcttctgg
     1201 atacagcgga tccgattgcg gcttggaaag atatccggca tgaacaggaa cgttgggtgg
     1261 attatttaaa caccaaaaag gaattgcgga tcgtttccga acataccgat attcgcgtgg
     1321 gaatcgtcgg caggaaatgg atcaattgca gcggtgaagt gaatttcccg gacggtgaga
     1381 tctttacttc cccgcttgaa aaccagattg acggttatat tacattcagc ttccctggaa
     1441 tctatatggg taaacagatc gaaggagttt acctggaagt caaggacggt aaggtgatta
     1501 cagcttcggc ccaaaaaggg gaggagttgc ttaagacatt acttgatacc gataccggcg
     1561 cccgttcctt cggtgaggtg gccatcggca ccaattacgg gattcaaaag ttcacccgga
     1621 atatgctctt tgacgaaaag atcggcggta cggtgcatat ggcggtgggt gattccctgg
     1681 ccgaggcggg aggaaagaac cgttcggcca ttcactggga tatgctctgc gatatgcggg
     1741 aatccggtaa aatttacgcc gatggggagc tcttttatga aaaagggaag ttccttgagt
     1801 aggcatgagg catgaggcgt gaggcacttc acacttcaaa cttctcttca ccctgtctat
     1861 gtaaggaggc tcagcccccg atgagttcaa acgtagtcga gtggatccgg gagtttctta
     1921 tagggtacca ggagaaatgg tgggaattga gtaccgatcc cccggagctg tcgctctact
     1981 ccattcggga acaacggacg ctggaacggg agctgaatca gtttacccgt cagtttccgg
     2041 aaggactctt gcgtctcctg gaacaggccg ggagtgattc agtttattta ggagaggccg
     2101 gtaaccggct ccgtgaattc acctgcagag tgcttccggg agcctcccat ttatgggaga
     2161 gttccactgc agggattccg tttttcgatg ccgccaccag tgcggcgcag gtgttcattc
     2221 aggcagccag agatttcgat ccggatattg ccccggccga tatctttcag gcattacgaa
     2281 atctttggac cgccaatggg ttacagctat gggccggata cccggtagcc tccactcagc
     2341 cgcttttggc atatagtctg ctttacccgt ataccgacaa ttatctggat gactcgcgga
     2401 tcccgctagg gattaagcag gaattcaacc ggaggttcca agattttttg gacgggggag
     2461 agtttgtacc caagaacacc catgaggcat ctttatcccg cctaatcggg atgattcgcg
     2521 acgcatatcc ggtcccagaa tacccccagg ttgcgcaagg cttgaatgga attcacgcgg
     2581 cacaggtccg gagcataaag cagttatttc ctctacccgg accgtatcat acagatatct
     2641 tggggattag ttttgaaaaa ggaggctgtt ccgtactagc cgatggattt ctgattcacg
     2701 gatcgctgaa ccgggatgag gcccaattat tgtttggtct tggtatcttg ctacaactta
     2761 tcgatgatct ccgagatttt gagattgatc gtcgccatgg ccatatgacg atattcactc
     2821 agacagctgc ggggggatgg ccgatagaca ccattaccaa ccgtttgttt gccctggctg
     2881 atcatttggc ggatcaggcc ggaaccttca atctcccggg agcagcaatt tttgaagatc
     2941 tgtttcaaaa aggttgccgg tttttgattc tggaagcgat tgcccgccat acccgcattt
     3001 ttacctcagc ttaccgaaat tactttacaa gccgttctcc ggtgcggctg tcttacttaa
     3061 gcaatttgaa accccgcttg aaacgacaat ttcattcaat aaatcacaac gaaactattc
     3121 caatggaaat gatgattcgg atgactgtgg cactcggccg ttgaccggga tgtgtatttt
     3181 tttccacatc ccggttaagc tagaaattgc cgaatgtgat cttctccccg aggtgagtcc
     3241 gatgcgtttc cgaaatcgag cctacgtatc attattgcgg atcataacca ttagtctgat
     3301 tattatttta accgtttggg gaacatttcg tagtccggaa atgatccgga ttttgaagca
     3361 gattgccgcc gcaccgtctg tgctctttac gaaaccggtc tatcgggtga gactggattt
     3421 ccaatatcgt gagtgtgatc atcaccgttt cacggaagcc agttttgcga gcctcaacca
     3481 gttaaaaacc aggctgcaga aatatggcca ttatcaactt cagaagcaat ccgatcgcca
     3541 ttatatctgt aggattggaa cagaagggtt ttgcgcttca tgccgggatt atcagttttt
     3601 agggattagc ggctctaatg tggccgtttt tagaggcaca ccacgtaaac ccggtccggt
     3661 gaaggagatt accgtattaa aagcggaaca acttccggcg acggaacgcc aagatctttc
     3721 gaagggcatc acttttcggg atgacaagga gaaactgcaa ctgctggaag gtttgaacgg
     3781 gttaatatct gcttgatgaa gcataagaaa agacccatcc cgaaaatcac agggatgagt
     3841 ctagagtctt tagcccggca tttcaatccc gattatagat cgcgttcttt cccatgcaat
     3901 cccggatccg ccggcatgga tcctttcatc ggcccatcac cgataatgtg gtgaggattg
     3961 cccaattgag attttaggtc tgtcaggact taaacctcaa tctctgttaa tctactcggc
     4021 cgggcatcaa ctctgttata tattatgatc gattcatccg tatttatgcc cctcttctca
     4081 ggtaaaattt tccgtaacat ccaaatggat gaaaaatatc ctaaaggatg aagcacatcc
     4141 taaaggatgt gaagcatcct ggtaacggag gaagaggata aaactttcag gtcaggcagg
     4201 aaaagaaggg attgaatcga atagttaaca attagtaaat gcgcgtccat ggacgcgaat
     4261 actaagttgc catcttccat ataaattagc aacttagtct tgtcgtgcgg gcgtccatcc
     4321 cacgagacaa gcttactcat tcggcgcctt cataattccc ctactttcgc acaaacataa
     4381 gtttggcggg caagcagaag atgcgattat gcagaatatg cagtctaagg agggtgtgta
     4441 ttgaagagat accaggtggg tcaaattcgg aatctgattt taatgggaca tggtggttgt
     4501 ggtaagacga
//
LOCUS       DASVMB010000185         5578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_169905,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000185 DASVMB010000000
VERSION     DASVMB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169905"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VF531_05565"
     CDS             complement(<1..614)
                     /locus_tag="VF531_05565"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7713470.1"
                     /translation="MESERFLGEALTFDDVLLVPAKSEVIPSEVNTVTRLTRQITLNI
                     PLISSAMDTVTEARLAIAIAREGGMGIIHKNMTIERQAAEVDKVKRSEHGIITDPVFR
                     SPEDTINDALRIMEHYRISGVPITAGGKLVGILTNRDLRFEINFEQPIKNVMTRENLV
                     TAPVDTTLEQAKAILQKHKIEKLPLVDKDFNLKGLITIKDIQKAV"
     gene            complement(863..1774)
                     /gene="ytxC"
                     /locus_tag="VF531_05570"
     CDS             complement(863..1774)
                     /gene="ytxC"
                     /locus_tag="VF531_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HEX7713471.1"
                     /translation="MSGITIGTLNLATDIKENLRREVHFLSREGINITLTEKENGKFF
                     FLDCTVAEEKDQAIKLAHEKILRYYLGNIITDLLMNGITKEFMSRILKNRYQYFSDKE
                     LKDVVQNAYSYLNNLHEEGDIAKTLFRHNQILMEVNEYLDSNSQLYLEGFFRFRLKSY
                     FGELEEAVETAVENYLVEQEYHEFIRLLRYFVDIQEPRIDEVHVLVKDKQSFYLLDED
                     QQPIDQEQLQGVLAELNQDVEYDDLLLSALITISPRRIILHVISPIEIVETIVNVFRE
                     RVRICEGCDLCRSLRKPKVLSNPVKVE"
     gene            complement(2109..2185)
                     /locus_tag="VF531_05575"
     tRNA            complement(2109..2185)
                     /locus_tag="VF531_05575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2149..2151),aa:Val,seq:gac)
     gene            complement(2365..3483)
                     /locus_tag="VF531_05580"
     CDS             complement(2365..3483)
                     /locus_tag="VF531_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713472.1"
                     /translation="MKKLFSLVLAGVLLALPTSFAWAVGLGYGARAIAMGGAYTAVAD
                     DGTAPYWNPAGISQIKILTLTPGIGAYGQWDMEKINKLINSMNSTDTPMEIPQIPPTQ
                     IGLPLTFGISTRSVALNLMVEGNLDLLDKDKYYKGNLDLTGYATLTGATTFDQLAIGV
                     NVKAIDARTMQGRTATMADPQNLTQAELDALMNSDYNYKILATGNGWSFDLGGLYTID
                     KQWKVGAVGRNLLSTVNWSGNIYTFVLDPSATASQGKFVFKNTNESYSATSSLPRTYA
                     LGASYKPYNSGLLAADVEIITADDADLNQTRLHLGFEQTALWNIFALRLGAFTEKNQP
                     IGMTAGLGLKLGPLMLDLAALKVGESEYGAFLTGGLKF"
     gene            complement(3663..3854)
                     /locus_tag="VF531_05585"
     CDS             complement(3663..3854)
                     /locus_tag="VF531_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HEX7713473.1"
                     /translation="MPDVNCTVDNCKYWTSGNLCEAQQIVIQNDAEGGFATNAKLKDL
                     SATPADSKDDTCCQTFNNQ"
     gene            complement(3957..4568)
                     /gene="lepB"
                     /locus_tag="VF531_05590"
     CDS             complement(3957..4568)
                     /gene="lepB"
                     /locus_tag="VF531_05590"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7713474.1"
                     /translation="MFEIKKTEYRENLESFAIAVLTILFIMVFIMRSFIVDGVSMEPT
                     LLNRERLLVNKMVFHFRQPRTGDIVVIIPPGDPTRKYIKRVIAGPKQSIRIENSKVYV
                     DGIELNEPYIKEKMTPEFYTGEVPDNAVFVMGDNRNNSTDSRVSSIVGFVPLKNVLGK
                     AFFVFWPLGKLHYLADPKYIPVYDSSFSMPESFGTGGSGISGQ"
     gene            complement(4702..5064)
                     /gene="rplS"
                     /locus_tag="VF531_05595"
     CDS             complement(4702..5064)
                     /gene="rplS"
                     /locus_tag="VF531_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7713475.1"
                     /translation="MNIIDAVEREQLRSDIPKFAPGDQVKVHVKVVEGGKERIQVYEG
                     IVTRRSHGGLKETFTVRKVSQGIGVERTFPIHSPMLEKIELDRRGKVRRAKLYYLRER
                     SGKSARIEERRDEQTTQG"
     gene            complement(5106..>5578)
                     /locus_tag="VF531_05600"
     CDS             complement(5106..>5578)
                     /locus_tag="VF531_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7713476.1"
                     /translation="RQLATVDHLILICGHYEGFDERIRAWADLELSIGDYVLTGGELA
                     AMVISDAVVRLLPGALGSEDSAVYDSFNGGLLDFPQYTKPPEFEGMNVPEVLLSGNHA
                     RIAAWRREQAIARTVERRPDLLAGADLTPLERKQLRDSGFPNPEDSKQKISFKD"
BASE COUNT         1416 a         1374 c         1297 g         1491 t
ORIGIN      
        1 accgctttct gaatatcttt gatagtgatt aagcccttta aattgaagtc cttgtccact
       61 aaggggagct tttcgatttt atgcttttgc aggattgctt tggcttgttc gagggtggtg
      121 tcgacaggag cggtgacaag attttcgcgg gtcattacat ttttgatggg ctgttcaaag
      181 ttgatttcaa atcgaagatc ccggttggtc aggattccca ccaattttcc gcctgcagta
      241 atcggcacac ccgagatgcg ataatgttcc atgatcctaa gcgcgtcatt gatggtatcc
      301 tccgggcttc gaaagactgg atcagtaata atcccgtgtt ccgagcgctt taccttgtct
      361 acctccgcgg cctggcgctc gatagtcatg tttttgtgga taattcccat cccgccttca
      421 cgggcaatgg cgatggccag tctggcttca gtaaccgtat ccatggccga actgatcaag
      481 ggaatgttta aggtaatttg ccgggttagc cgggtaaccg tatttacctc gctggggatc
      541 acctcggatt tggccggaac cagaagtaca tcatcgaatg tcaacgcctc gcctaaaaac
      601 cgctcacttt ccaaaacctt cacctcattt taatatcaat tcagaaaaaa cacgtgcgat
      661 cgaatgtttg gttcaaatgg tgatcaaaac tttatgcgga ttgatttgga acccgcgccg
      721 taccaaaatt attgcttcta ttctaccata gaaattgata tttgcaagcc ataaatgatc
      781 aaagatcatg aattcgtgat cttttacgac ggataaaaaa aacccgtcct cttcggacag
      841 gattcgttat ttgttagtgt actcactcga ccttgacggg gttggacaag accttcggtt
      901 ttcggagcga acggcagaga tcgcagccct cgcagattcg gactctttcc cggaagacat
      961 tcacgatagt ctccacaatc tcaatgggtg aaatgacatg gaggatgatc cgccgcggcg
     1021 agatggtgat gagggcgctt aacaacagat cgtcatattc tacatcctgg tttagctctg
     1081 ccaatacccc ttgaagctgt tcctgatcaa tgggttgctg atcctcatcg aggagataga
     1141 aactctgctt atccttaacc agcacatgaa cctcatcgat ccgtggttcc tggatatcca
     1201 cgaaataccg gagcaacctg atgaactcgt ggtattcctg ttccaccagg taattttcga
     1261 ccgcggtttc gaccgcctcc tccagttcac caaagtagct tttgagccgg aagcggaaga
     1321 acccttccaa atagagctgg gagttggagt cgaggtattc attgacctcc atcaggatct
     1381 gattgtgacg aaaaagcgtt ttcgcgatat cgccctcttc atgaagattg ttcaggtagg
     1441 aataagcgtt ctgcacaacg tccttcaatt ccttatcgct aaaatactgg tacctgttct
     1501 ttaaaatcct gctcatgaac tctttggtaa ttccattcat gagtagatcg gtaatgatgt
     1561 ttcccaaata ataacggagg atcttttcat gagccagctt tatcgcctgg tccttttcct
     1621 ccgccaccgt acaatccaga aagaaaaatt tgccgttctc tttctcagtc agcgttatat
     1681 taatcccctc ccggctgagg aaatgaacct cgcgccgtag gttttccttg atatcggtcg
     1741 ccagattgag ggtgccaatc gtgattccag acatattaaa ccctccgtta tccctgtaga
     1801 gcacaaaatt aattgtcaaa ccgcgtcata tacggcgata agctcacagg gaatagtcag
     1861 tcgccgttca tagcactatt atatgagtgg atttttcttt gtatactgta ggaacaaagg
     1921 aataaaccct gcatcttccc actcgatagc aggcgtgagg cacgagacgt gaggcgtgaa
     1981 taggcatcgc gtccaatgga cgcgcggcaa cgggcaatag attaaagtgt tttactcttg
     2041 cctattgcct gttccctgtt gcccataaaa aaacaaggcc attcggcctt gtttagtaag
     2101 caaatacatg gtggggaagg ttgggatcga accaatgacc tccacgatgt caacgtggcg
     2161 ctctaaccaa ctgagctact cccccgcagc aaaatttatt ttaccttacc ccatcccgtt
     2221 tgtcaaggga aaatggatcg aaaaaaggat agcaaatact cagttgcttt cgattgtcaa
     2281 aaacaactga gtatttcttg agactaattt gcattcaatg gttgaatgtt aatcagtcta
     2341 ttctgaaaat agaagcttga ccggttaaaa tttgagtccg ccggtgagaa aagcgccgta
     2401 ctccgattct ccgaccttaa gagctgccag gtccaacatc aatggaccga gtttcaatcc
     2461 caagccggcg gtcattccta tgggctggtt cttctcggtg aaggctccca accgtagggc
     2521 gaagatattc catagcgcgg tctgctcaaa acccaggtgc aaccgggtct gattcaaatc
     2581 cgcatcgtcg gcagtgatga tctccacatc cgcggctagt aaacctgaat tatacggctt
     2641 gtaagaggca cccaaagcat aggtccgtgg caaactggag gtggcagagt aggattcatt
     2701 ggtattttta aaaacgaatt taccttggga agcagttgca cttggatcaa gaacaaatgt
     2761 atagatattc cccgaccagt ttaccgtgct cagcagattg cgtccgaccg ccccgacctt
     2821 ccattgtttg tcaatggtgt ataatccacc gagatcaaac gaccaaccgt ttcctgtagc
     2881 aagtatctta tagttataat cactattcat taaagcatcg agttccgctt gggttagatt
     2941 ttgaggatca gccattgtgg cggtacggcc ctgcatggtt ctggcatcaa ttgcttttac
     3001 attgaccccg attgccaatt ggtcgaaggt tgtagctccg gtgagagtcg catacccggt
     3061 taaatccaag ttgcctttgt agtatttatc tttatccaac aggtcaagat taccttcaac
     3121 catgagattt aaggcaactg aacgggtaga gattccgaaa gtgagcggta ggccgatctg
     3181 ggtcggagga atctgaggaa tctccattgg ggtgtcggtt gaattcattg aattgatgag
     3241 tttgttaatt ttctccatat cccactgacc atatgcccct attcccggag tcaaagtaag
     3301 gatctttatc tggctgatcc cggccggatt ccagtagggc gcggtcccgt catcggcgac
     3361 tgccgtataa gctccgccca tagcgatggc ccgggcgccg taacccaggc cgactgccca
     3421 ggcaaaggag gtaggcaggg cgagcagtac gcctgccaaa acaagactaa atagtttttt
     3481 cataattttc acctcctctt cactttatta cctagatctt cggtgatttt ttactgtttc
     3541 ttaagggata aatttcctga agttctttac cgaagaggaa aaggaggaat tctggtcacg
     3601 gatgcagtag aaagccggtc caaaaaatct tggaccggcg ggagccataa cggcttaggt
     3661 gtctattggt tattaaaggt ttggcagcag gtatcgtcct tggaatcagc tggagtagcg
     3721 cttagatcct ttagcttcgc attggtggca aaaccaccct cggcatcatt ttggatcaca
     3781 atctgttggg cctcacaaag attaccgctg gtccagtact tacaattatc cacggtgcaa
     3841 ttaacatcgg gcatactctt cacctcttca aaaattgttg gatatagagt gcccaaagat
     3901 gaaaatccca ttcaatctaa ggataggtta aagcgttaaa gtcttgcgac ccgggtctat
     3961 tggccggaga tccccgaccc gccggtaccg aaggattcag gcatcgaaaa cgagctgtcg
     4021 tagaccggga tatatttcgg atcagccaga taatgaagct tacctaaggg ccaaaagaca
     4081 aaaaaggctt ttcccagtac attctttagc ggtacgaaac ccacgatgga actaacccgg
     4141 ctgtcggtac tgttattccg gttatccccc atgacaaaca ccgcgttatc cggaacctcg
     4201 ccggtgtaga actccggagt catcttttcc ttgatgtagg gttcgtttag ttcgatcccg
     4261 tctacatata ccttggagtt ttcaatccgg attgactgct tgggtccggc gattacccgt
     4321 ttgatatact tccgggtggg gtcacccgga ggaatgatca ctacgatatc tccggtccgg
     4381 ggctgccgga agtgaaagac cattttgttg acgagcaacc gttcccgatt gagcagtgtc
     4441 ggctccattg aaaccccgtc cacaatgaat gatcgcataa tgaagaccat gatgaataag
     4501 atcgtcaata ccgcgatggc gaaagactcc agattttcgc ggtattccgt tttttttatc
     4561 tcaaacatgg aacggcctcc ttcagaggtg agatgttgcc atgaccaaaa aagagacgcg
     4621 cgtgcgattt agcgacacgc gcgcatagct aaccccgccg gaataatgca tttccggtta
     4681 aatccttcca caatgaccag attacccttg ggttgtctgt tcgtcgcggc gctcttcaat
     4741 ccgggcggat ttgccggaac gttcccgcaa gtaatagagt ttggcgcgac gtactttgcc
     4801 gcggcggtca agctcgatct tctccagcat cggggagtga atcggaaaag tccgctccac
     4861 accgataccc tgagatacct tgcgtacggt aaatgtctct ttcaacccgc cgtgacttct
     4921 gcgggtaacg attccttcat aaacctggat ccgttcttta ccgccttcaa ctaccttaac
     4981 atgtactttc acttgatcgc cgggagcaaa tttcggaata tccgatcgta attgttcccg
     5041 ttcaactgca tcaatgatgt tcatgagaaa cctcctctct ctgggcttga aataattgat
     5101 aaggtttaat ccttaaaaga aatcttttgt ttcgagtctt ccgggttcgg aaaaccggag
     5161 tcccggagct gtttacgctc aagtggcgta agatcggcac cggccagtag atcgggacgg
     5221 cgttccacgg ttcgggcgat agcttgctcc cgtctccaag cggcaattcg ggcgtgattt
     5281 cctgagagca gaacctccgg aacattcatg ccttcaaact ccgggggttt ggtatattgc
     5341 ggaaaatcta acaaaccacc gttaaatgag tcgtaaactg ctgaatcctc cgaacccaga
     5401 gctcccggaa gcaaccggac gaccgcatca ctgatcacca tagcggccag ttccccgccg
     5461 gtcaacacat aatcaccgat ggataattcc agatcggccc aggctctgat ccgctcatca
     5521 aacccttcgt agtgaccgca aatgaggatt aaatgatcaa ccgttgccag ctgtcttg
//
LOCUS       DASVMB010000186         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_24306,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000186 DASVMB010000000
VERSION     DASVMB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24306"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VF531_05605"
     CDS             <1..518
                     /locus_tag="VF531_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713477.1"
                     /translation="GLLLIASKKRLLQRYATQTFNYDFPEALLEGMNAGELVAWMTET
                     EGTFSIAVNIGDTFDYGAMEKAGWQAMKNHRLFIEDDDTLLILPYSQYSFACDQNKGQ
                     VEIIEGLSGVMSISKGWYRVDIVGALHDPSLTDEDEEEDEDVSGEFIIFLTPSIPVRD
                     KESYPDIPRFS"
     gene            complement(656..2029)
                     /locus_tag="VF531_05610"
     CDS             complement(656..2029)
                     /locus_tag="VF531_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7713478.1"
                     /translation="MFTEFGNQLFREIEIKVNWLNLIMLFAFVIFEAIMMVISHLPTV
                     TVIAVSIGGLLVLSLPTWLLIKKPSPANKYIIYTLALIFVLLANYLNAQSSIFLSYFL
                     IGFFIVVNFIVLTLFARQLIKNLDHQEQKVIDFFTGINQLMQLMQDSAEAMTASSQEM
                     ASKAEETNAQAAEITASIDYLNTDAKENSEHMNWTKSTLEKLVQQANEKITLTMQTTQ
                     MILEIIDATQSGLSDSSNVAESIERINKQLEGMQTIIQDLNVNSETISNITDSIREVV
                     DQIALLSLNAQIEAARAGEHGKGFAVIAQNIARLSDESKQASITIQELISQIVVLIRN
                     TVQQSTTTGRLIQESFTRTANLISNFNQIQNSLVTSQPAITEFQSYITSQIEIINQIA
                     EHVTEANQFALTSEQAMMGINESMQQLETMSEELASNANQLSAQAQSIAGTLEDVNTK
                     RQDIEVA"
     gene            complement(2128..2556)
                     /locus_tag="VF531_05615"
     CDS             complement(2128..2556)
                     /locus_tag="VF531_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713479.1"
                     /translation="MSIGRIIVFGMYSIVAALTFLFGVIYLTRKQFMPYHAQAVETPW
                     EKVDSKFQVLLIALLKVAGGGMLSNSLAIVCLLFLAFMPGAGWANPALFVISLSEGLA
                     NLWATSIVKFKSKANPPWLLSLIIVCLSVAGFILGFIFPK"
     gene            complement(2570..3484)
                     /locus_tag="VF531_05620"
     CDS             complement(2570..3484)
                     /locus_tag="VF531_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7713480.1"
                     /translation="MAKSNSSQKVCVIGAGFMGFQIALHSAAHGCEVWVVDTAESMLA
                     KAKQLSEKELAQRVTNQRISTTEKEQILNQLHFTLKLDDSKTADIVIEAIPESLPLKR
                     DLFAKLDQIYPSKTIFATNSSSLMISEIEDAVSPERQKQMVNTHFYPPVWQRPMVEIM
                     KGSKTTPQIYQQVVEFAQSLGLAPITVQKESTGYVFNRLWRAIKKESLTIVDEGVASP
                     EDIDRAWMIAFGLPMGPFANMDMVGLDVVLDIERVYHRKSGLSADAPPQFLIDMVAGN
                     ELGQKTGKGFYTYPNPAYKDPAWLKNQK"
     gene            complement(3509..3880)
                     /locus_tag="VF531_05625"
     CDS             complement(3509..3880)
                     /locus_tag="VF531_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713481.1"
                     /translation="MDRTKKIQHQGKDIVSVDFSNLGRQSLPEIMNVIDGAATLIRCN
                     PPQSVLVITICTGMMFDIEIMKLIGEYVAGNKPYIKASALVGADGLLRSALSNAKRVS
                     GRNDLNLFQSTEEALNWLATQ"
BASE COUNT         1075 a          870 c          924 g         1233 t
ORIGIN      
        1 atgggttgct gttgattgca tcaaagaaga gattattgca acgctacgcc acccaaacat
       61 tcaattatga ttttcccgag gctttgcttg aaggaatgaa tgccggtgaa ctcgttgcct
      121 ggatgaccga aacggaaggg accttcagta tcgctgtcaa tattggcgat acctttgact
      181 atggggcaat ggagaaagcc ggctggcaag caatgaagaa tcatcgcctg tttattgaag
      241 atgacgacac actgctgatt ctgccatatt ctcaatattc ctttgcctgc gatcaaaaca
      301 aagggcaggt agaaatcatt gaaggattaa gcggtgtgat gtctatttca aaaggttggt
      361 atcgtgtgga tatcgtgggc gctttacatg acccgtcttt gactgatgag gatgaagaag
      421 aggatgagga tgtaagtggt gaattcatca tttttcttac tccgtcaatt ccggtacgtg
      481 ataaagaatc atatcccgac attccgaggt tttcctaatt caccgattcc cgcatcatat
      541 ttttacatgt ttggccatga tgaagcaata tgtattttgg aattcacctt cgatagccca
      601 aaaaattaac ccggtataaa ttaagccggg ttttgcgatt cttaaaaatg tggaattaag
      661 ccacttcgat atcttgtctt ttggtattca catcttcaag tgtgccggca atcgactgag
      721 cttgagcaga caattggttg gcgttactgg caagttcttc cgacatggtt tccaactgtt
      781 gcatcgattc attaataccc atcatcgcct gttccgaggt taaggcaaat tgattggctt
      841 cggtcacatg ttcggcgatt tggttaatga tttcgatttg ggatgtgata taactctgaa
      901 actcagtgat tgccggttga ctggtaacca gtgagttttg tatctggttg aaattgctga
      961 tcagattcgc ggtgcgggtg aaactttcct ggatgagtct gccggtggtt gtcgactgtt
     1021 gaactgtatt tcgtatcaat acgacaattt gactgatcag ctcttgaatg gttatcgatg
     1081 cttgtttgga ctcgtccgac aggcgagcta tgttttgggc gatcaccgca aatcctttgc
     1141 cgtgttctcc ggccctggcc gcttcaattt gggcattgag ggacaataag gcgatttgat
     1201 cgacgacttc cctgatactg tcggtaatat tcgatatcgt ctctgaattg acatttaaat
     1261 cttggataat ggtctgcata ccttcaagtt gcttattgat cctttcgatg ctttcggcca
     1321 cgttggaact gtcacttaat ccggattggg tcgcatcgat aatttccaga atcatttggg
     1381 ttgtttgcat cgtaagagta atcttttcgt tggcctgttg aactagtttt tcaagggtgg
     1441 attttgtcca gttcatatgc tcggagtttt ccttggcatc cgtatttagg tagtcgattg
     1501 aggccgttat ttcggcggct tgggcattgg tttcttcggc ttttgatgcc atctcctggc
     1561 tggaggcagt catggcttcc gcgctatcct gcataagttg catcaattgg ttgatgcctg
     1621 taaaaaagtc gatgactttt tgctcctggt gatccagatt tttaattaac tggcgcgcaa
     1681 aaagagtcaa tacgatgaaa tttaccacaa taaaaaagcc gattaaaaaa tagctgagaa
     1741 aaatactgct ttgagcattt aggtagttgg ccaataaaac aaatatgagt gccagtgtat
     1801 agatgatata cttgtttgcg ggtgaaggtt tttttatcag taaccaggtt ggtaaggata
     1861 aaaccagtaa acccccaatc gataccgcta taaccgtaac cgtaggtaga tgcgatatta
     1921 ccatcataat ggcttcaaaa atcacaaatg caaaaagcat tatcaagttt agccagttca
     1981 ctttgatttc tatctcacga aagagctgat tcccaaattc agtaaacatg cccattcgcc
     2041 cccttcgctg ctggatccgg ctgccgtatg gcagccggat cccatgatta caaaatggta
     2101 ccccaagtat tcaaagtact ttttatttta ttttgggaat ataaatccca aaataaaacc
     2161 ggccaccgat aaacatacga tgattaacga tagtaaccaa gggggattcg ctttcgattt
     2221 gaatttgacg atcgatgtcg cccacaaatt ggctaaaccc tccgaaaggc taatcacgaa
     2281 aagcgccggg ttggcccatc ctgcgccggg catgaacgcc agaaaaagga gacagacaat
     2341 ggccagggaa tttgagagca tacccccgcc ggcgactttc aacagagcga tcaacagtac
     2401 ttggaacttg gaatcaactt tttcccaggg ggtttccacg gcctgggcgt gataaggcat
     2461 gaattgcttc cgggtgaggt agatcacccc aaagagaaag gttaatgcag caacgatgga
     2521 atacattcca aatacgataa tccgaccgat cgacatcaaa attgcctcct tatttctgat
     2581 tttttaacca ggcgggatct ttgtaagcgg ggttgggata tgtataaaac ccttttccgg
     2641 ttttttgccc caattcgttt ccggccacca tgtcgatgag aaattgtggt ggcgcatctg
     2701 cgcttaagcc cgatttccgg tgataaaccc tttcgatatc gagcactacg tccaatccca
     2761 ccatatccat attggcgaaa ggccccatcg gtaaaccgaa ggcgatcatc caagcccggt
     2821 caatgtcttc gggagacgca accccttcat ctacaatggt tagactttct tttttaatgg
     2881 ctctccagag tcggttgaaa acatatccgg tggactcctt ttgaactgtt atgggagcca
     2941 gcccaagcga ttgagcaaac tcgaccactt gttgatagat ctgcggggtt gtttttgagc
     3001 ctttcataat ttcaaccatc ggccgctgcc aaacaggagg gtaaaagtgg gtattgacca
     3061 tctgtttttg gcgttcgggg ctgacggcat cttcgatttc ggaaatcatc agggaagagc
     3121 tattggttgc aaagatggtt tttgaagggt agatctgatc cagcttggca aacaaatccc
     3181 tttttaaggg cagtgattcc ggaatggcct caatcacgat atccgccgtt ttggaatcat
     3241 ccagcttcag cgtaaaatgg agttgattca agatttgttc cttttcagtt gttgaaatac
     3301 gctggtttgt gacgcgctga gccaactctt tctcagagag ctgctttgcc ttggccagca
     3361 ttgactcggc agtatcgacc acccatacct cacatccatg tgccgcagaa tgcaaggcaa
     3421 tttgaaaacc catgaaaccc gcgccaatca cacaaacttt ttgagaactg ttgcttttgg
     3481 ccatttatat atcttctcct taccgttatt attgggttgc taaccaatta agggcctctt
     3541 cggttgactg gaaaagattt agatcattcc gacccgatac tcttttggca ttactaaggg
     3601 cggaacgaag taacccatct gctcccacca gggcagaggc cttaatgtat ggtttgttac
     3661 ccgccacata ttctccgata agtttcatga tctcaatatc aaacatcata cctgtacaga
     3721 ttgtaatcac gagtacggat tgggggggat tacaccgaat taacgtcgcg gctccatcaa
     3781 ttacattcat gatttcaggg agtgattgtc tgcctaagtt tgaaaaatca acacttacaa
     3841 tgtctttacc ctggtgttga atttttttgg tacgatccat cgattttacc tccatttttt
     3901 atgacttaac cgttggcgta aatgtaaata tctatcgttg tcagatcggt tgattctacg
     3961 aagcctccct tcgttatgaa atgttttaca tggtaccgga tgtatcaata aatcgatctg
     4021 aatattttga aattaattaa taggcggttt taaatgttat ttaaaaagtt ctttcattga
     4081 ttggctgagt attgcataaa gt
//
LOCUS       DASVMB010000187        11595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_170067,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000187 DASVMB010000000
VERSION     DASVMB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170067"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..1292
                     /locus_tag="VF531_05630"
     CDS             588..1292
                     /locus_tag="VF531_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX7713482.1"
                     /translation="METSVQVNQEQSRLGFFARLGNLFSAPSRVFAQLREKPDLIWPL
                     IFFAVMAGLSLATASFNQKLLADSFPKYAAILSQAQAQKSTFALIGGAVGSLVGLAFF
                     WVLGTGIYYLMAKIVGGESVKFVAFLAMVGYTNIATLLQTAFQSGVLALTGSIPPVGL
                     ETGMELSDRFFTTKGLLLSSINPFAIFGIILIIIALEKAFKISRTKAVSIALIYWVAS
                     IAISIGLSTFSFSQLR"
     gene            1358..2737
                     /locus_tag="VF531_05635"
     CDS             1358..2737
                     /locus_tag="VF531_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7713483.1"
                     /translation="MRKIILTLLIIFTCVSGLAGVIAAAEEGQLLTPDQAVAGSLDSD
                     SSLRAAQLAMEVAADEYNAARRIFSLGGSYSQSQSDPVDIDSSTESLMINSGGTSGDP
                     NAWWSLSASQSDASLGNTDVKPGQTYNLTLTPFNLSQAINTKSKEVALAGKTQAYETT
                     KITLSTGIRTTYAEVVEKAALLQIAREDLDLTKAHLKQVNVFYNVGKIAKLDLMDAEQ
                     QVKASEAKLASADLSYQTALLKLSIALGKPDLKGYTFRSEALKGIGADQIDIQQTLDR
                     CLKDSPEVKGYKTQMELAGLNLRMSYFFQLSGLQLGASVYKPKDPLEGQPKDVTTYSL
                     KIQGALDDSPFRNLAMAKKNFKAAQDTLDFTVKTMQTKVLDSLRLWKMAELSLEPMDQ
                     TINVAKERLRIANLKYDNGMASTSEVNETRKALIASEEAYWQSWYNLIQAREGFYQAV
                     WGNPAVKNQ"
     gene            2829..3992
                     /locus_tag="VF531_05640"
     CDS             2829..3992
                     /locus_tag="VF531_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7713484.1"
                     /translation="MKKNMKRWLVIGGVVAVIIAYVFIQVQAGMKKSSAGVMVDLSKL
                     QRTNLVTTVSGSGQVDAEDKAQLRAEINGLVTKVLVKEGQRVRKGDLLVQFDDRQYVN
                     ALQTALVDEQALEQSKIHLASTRKTYQHTLELFKNGAASQEQLDQAELTLSQEEKQVA
                     QSTQLAGIKLSNARLDLDRTKVRATRNGIILFCPAKEGMAVTPGTELCQIGELSKLVV
                     ELPVDEIDITQIKLGQPATITQEGLTELKLKGTVIAIAPQAVKNQNENVFPVKIRIDN
                     SSGMLRSGMSVDTEIVTNDLKDVLTVPLLAVLEEEHKGKTEKYLFKVIDGKAVKTKIT
                     AGFSSDSELEVLGGIKEGDTYVSGDYETILHLKDKTAVRQRVLKPQAQPKANQ"
     gene            4020..4742
                     /locus_tag="VF531_05645"
     CDS             4020..4742
                     /locus_tag="VF531_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713485.1"
                     /translation="MMIRIQNLNKIYKNGSLEVQALFDINLEVSEGEFVAIMGPSGSG
                     KSTLMNILGCLDRPTNGSYQLKGVEVGGLNDDQLAEIRNKRIGFIFQTFNLLPRSTAL
                     QNVELPMVYAGVHRGERHQKAMDALKMVGLAERCHHRPNEMSGGQRQRVAIARALINN
                     PSIILADEPTGNLDSVSGREIMMILQALNEQGITIILVTHDNEIGRHAKRIVRFRDGR
                     IVSDEPVSDRIEIGSESHDAAG"
     gene            4726..5925
                     /locus_tag="VF531_05650"
     CDS             4726..5925
                     /locus_tag="VF531_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713486.1"
                     /translation="MMPQGEWIKMACTNLWNNKLRSFLTLLGIAIGIGAMIALIGIGY
                     GFKASMLKQFESIGTNTIMVFPSSYQETGKKMKTLKERDRRVIQSVCGNDAAVVPFIF
                     DNGRAISYRGKSTSPGITGTSGDFNAVESLKLKYGRFLNQSDVAAGSRVVVLDWGVAE
                     KLFGSMNPVGKNVQLYNRSFRVIGSLEKRDSSVSMGSGSGGKNLYLPVTTLQRMMNFY
                     EYYGLYVSVSDMSKTDTVMEKLRGILAIRYGSADYFQIFSTKQLLQMITTVMGVITTL
                     LGAIGAIALVVGGIGIMNIMLVTVAERIREIGVRKAIGAKKKNILWQFLLESVFLCLL
                     GGTLGTGIGFLASFIISKLAPLQPVITPGLIVTAFIFATVVGLSFGVFPAWKAAKLDP
                     IEALRHE"
     gene            5941..7143
                     /locus_tag="VF531_05655"
     CDS             5941..7143
                     /locus_tag="VF531_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713487.1"
                     /translation="MQISEALFMALNQLKANKMRSFLTLLGIIVGIAAVIALMAVGNG
                     AVEMISGQFSAAGTNMVFIQWDYGNENNDDLVPQLLQDKDLAAIRAGGDLASNVVPYN
                     QTASEVRAGRETLAVTITGINEGFLELQGLKLARGRFFNSFEVTSAKSVCVLGWDLHD
                     KLFKNSDGLGKTIRLRGKKFLVIGIMEKKDDRSFVNTGLTDNMRLYIPYKTLQRMWNV
                     KGYPVIMAQPIDITITKALVGQIKWIIARIYGPKNNFLVQSMEQILEQTLSRMQIFSI
                     FLAGLGGISLLVGGIGIMNIMLVSAKERTQEIGVRKALGAKRKQILLQFLIEAIVLCL
                     IGGFLGIALGFLIAVGIKATNLIPAAVSLSSVLLAVGCSLAIGLFFGIYPAWKAAQLD
                     PIESLRYE"
     gene            7307..7465
                     /locus_tag="VF531_05660"
     CDS             7307..7465
                     /locus_tag="VF531_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX7713488.1"
                     /translation="MKKWQCTVCGYIYDPEKGDGGGVPPGVAFEDLPDDWTCPECGAG
                     KDLFEPVD"
     gene            7519..8700
                     /locus_tag="VF531_05665"
     CDS             7519..8700
                     /locus_tag="VF531_05665"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HEX7713489.1"
                     /translation="METHELAPNVWWIGALDPQLRVFDIIMRAEHGTSYNAYMVKGSR
                     GIAVIETVKSGFFREFIDEVKSLADPAQIKYIILNHMEPDHSGSIGELLKLAPEAQVV
                     VSRTGEHFIKHILNYQPKLMKMGDGDRIDLGDKHLRFIAAPFLHWPDSMFTYLEEDQI
                     LFDCDFLGAHYADPMLFGDQDEGFLEDFKYYYDHIMRPFKPYVIQALDKLAAMPLAMV
                     APGHGPVIRHDIPKYLGYYREWSMANREEAKVVIAYVSAYGNTAKLALKLAEGIQASG
                     VPAEIYNLEQVAPGELVDKIEAAKGLLVGSPTINGDAVKPAHDLLSSLATISLKGKLG
                     AAFGSYGWSGEAVSILESRMAGIKFKVMNSTVRPVLIPTEADLANAVEFGKAFGAAVR
                     G"
     gene            complement(8705..9220)
                     /locus_tag="VF531_05670"
     CDS             complement(8705..9220)
                     /locus_tag="VF531_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX7713490.1"
                     /translation="MTPKQTPAQKDPGTPPDKFSMILFSGDFDKAMAAFTLANGAAGR
                     GMEVTVFFTFWGINLLRRRFLSGDNLLENLFKRLMPLGIERIGLSKMNFCGIGPRLMK
                     KLIRDKDGQTARDLFQMAMERKVRFIACEASLKLLGIRREELIEYDRLEVAGVDTFIQ
                     NAMESKISLFV"
     gene            9348..10250
                     /locus_tag="VF531_05675"
     CDS             9348..10250
                     /locus_tag="VF531_05675"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX7713491.1"
                     /translation="MIPYHELTLADKASFDRILSVLQPESSDLTFTNLFMWQYNYNLH
                     SLYLQDLDFWVLLARPTQWKAFYLPPLGDWTDNRKLGAVLQKMRDWAEADGFEFRMRR
                     IPEPLAQALERIDPALALTPEPNTSDYVYRAQDLITLAGRKYHGKRNHLNQFQRKYAW
                     EYQRMTPEIVEECLQSDKEWFNICGALERDGATDEEKAMALVMQNYAALGLTGGVIRI
                     NGRIEAVSVGERLTSNMAVIHIEKANTEFDGTYTGINQQFAADAWKDFEFINREEDLG
                     LEGLRKAKESYHPEKMVEKFGVKY"
     gene            10690..>11595
                     /locus_tag="VF531_05680"
     CDS             10690..>11595
                     /locus_tag="VF531_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713492.1"
                     /translation="MGTNKYFKTLKIITISFILFTLIAISDLIPNVKGSIRNYTFRFT
                     DSTQGSTNCNTMSAYFPSDCSQGTHDFAGLGWRASRYVGGNLTVCPYRITMNYSVHFD
                     GSFPSNSIAPWFRISRCDANGNISQYGDVIYYSDGNGITNYSVSLEPYQGIYVEMNSG
                     ENWTRLGLNKIYQGYTCNFSVNYNIDDVKPLAPTGFGIQDNQQYSYNDVIYTKTNPLS
                     FKWNKGVDQGNPVSAVDGYYLYYGNDSQWIDEPQTVFSDNKGWFSNEGKYTVQLKVKD
                     NAGLLSDLASLTFTVDRTINQVTFPS"
BASE COUNT         3015 a         2566 c         3120 g         2894 t
ORIGIN      
        1 cgccaaggtg gtcaaggcgg tcagggaggt caaaccggcc ggaactgggg cggcgggaac
       61 tccgggacga acaatggggc gcgccaggaa gcgatttcag caggcagggc gcttttgatc
      121 aaggtgaata acgaagtcat gaggctgttg cggcaataaa ggaactgacc gggattgatg
      181 ttaagggatt tttagcacat agttaaggca ggaaaaggtt cttaaccacg aaaagcgcga
      241 aacgcacgaa aaaggagcag attccttcgt gcaatttcgt gtatttcgtg gtttgttatt
      301 gtatttaccc gggtaataga ggccttcaaa ataaagaata accggggatt taggcgtcaa
      361 aggatgggtt aaccttggct gccaggatta ttattgtcaa acaaatttta acaattatta
      421 attgtttacc ccggatactt atagtacaat aagataaata atcagaccca aaggcctgat
      481 aaatttagct ctttgcgtct ctacgcgact cttgagtata cagtattttt atatcacctt
      541 atttgagaca atggactcta tttgtgaagg aaaaaggagg aatccatttg gaaacttctg
      601 tacaagtaaa tcaggaacaa tcccgactcg gcttttttgc ccgcttgggc aatctatttt
      661 ctgctccgtc gcgagttttt gcccagttgc gtgagaaacc ggatctgatt tggcctttga
      721 tattttttgc ggtgatggcc ggactttcac tggctaccgc tagttttaac cagaagttac
      781 tggcggatag tttcccgaaa tatgccgcca tactctcaca agctcaagca caaaaaagta
      841 cttttgcttt gattggcgga gcagtcggtt cattggtcgg tttggcgttt ttctgggttc
      901 tggggaccgg catctattat ttaatggcca agatcgtcgg cggggagtcg gtgaagttcg
      961 tcgcgttcct cgcgatggta ggttacacga atatcgccac ccttttgcag acggcctttc
     1021 agagcggggt gctggctctc accggaagca tcccgccggt aggactggag accggaatgg
     1081 agttgagcga cagattcttt accaccaagg gactgttact ctcatccatc aatccttttg
     1141 cgatcttcgg aatcatcctg atcatcattg cgctggagaa agcctttaag atttcccgga
     1201 caaaagcggt gagtatcgcc ttaatatact gggttgcatc catcgcaatc tccatcggat
     1261 tgtctacttt ttcttttagc cagttacggt aaacggcctg tgatgatgtt tagtaaggat
     1321 ggcccgttac acgggccatt tttgtggggg gatttttttg cgtaaaatca tattaacgct
     1381 tttgattata tttacttgtg ttagcgggtt ggcgggggtg atcgccgctg cggaggaggg
     1441 tcagttgctc actccggatc aggcggtggc cggctccctt gacagtgatt cctctctacg
     1501 agcggcacaa ctcgccatgg aggttgccgc ggatgagtac aacgccgccc ggaggatttt
     1561 cagcctcggc ggcagttata gccagagtca aagcgatccc gtagatatcg atagttccac
     1621 cgaatcgttg atgatcaact ccgggggcac ctccggtgac cccaacgcct ggtggtcatt
     1681 atccgcttcc cagagcgatg cctctttagg caatactgat gttaaaccgg gccagactta
     1741 taatctgact cttacaccgt ttaatctcag tcaggctata aataccaaga gcaaggaagt
     1801 tgccctggcc ggcaagacgc aggcatatga gaccaccaag atcaccttga gcacggggat
     1861 ccgtaccact tacgccgagg tagtggagaa ggctgcgctg cttcagattg cccgggaaga
     1921 tctggatttg accaaggctc atttaaaaca ggtcaatgtc ttttataacg tgggcaagat
     1981 cgccaagctt gatttgatgg atgccgagca acaggtcaag gcatccgaag ccaaactggc
     2041 cagtgccgat ctgagttacc agactgcgct gctcaagttg agtattgcgc tgggtaagcc
     2101 ggatctgaaa ggctacacct tccgcagcga ggcgttgaag ggtattggcg ccgatcaaat
     2161 cgacattcag cagacgctgg atcgctgcct gaaagactca ccggaagtca agggctataa
     2221 aacccagatg gaattggccg gcttgaatct gagaatgtcc tacttctttc agctgagcgg
     2281 tcttcaattg ggcgcttcgg tatacaaacc aaaggaccct ctggagggtc aaccgaagga
     2341 tgtcactaca tattcattga agattcaggg agcgctcgat gactcgcctt ttcgtaatct
     2401 ggcgatggcc aagaaaaatt tcaaggcagc ccaggatacg ctggacttta cggtgaaaac
     2461 catgcaaact aaagttttgg attcgctgcg cctgtggaaa atggccgaac tcagcctgga
     2521 accgatggat caaaccatca atgtcgccaa agaacggctc cggatcgcca atctgaagta
     2581 cgacaacgga atggcctcga cttcagaagt aaacgagacc cggaaggcgt tgatcgcatc
     2641 ggaggaagcg tattggcaaa gctggtacaa tttgattcaa gccagagaag gtttttacca
     2701 ggcggtttgg ggcaatccgg cagtaaaaaa ccagtaatca gagggataaa taaccctaca
     2761 attgttgaaa acctgcaaag ctcaatttga ataccaggta atattgcagg gaaatgggag
     2821 agtgtaccat gaagaagaat atgaaacggt ggctcgtcat cggcggggtg gtcgcagtca
     2881 tcattgcata tgtgtttatt caggttcagg ccggtatgaa aaagagcagt gccggagtaa
     2941 tggtggatct cagcaaactt cagcgaacca acctggtgac gaccgtctcc ggcagcggcc
     3001 aggtagatgc ggaagacaaa gcccagttgc gggcggagat caacggactg gtcaccaagg
     3061 tgttggtcaa ggaaggtcag cgggttcgga agggcgattt gttggtgcag tttgacgaca
     3121 ggcagtacgt caatgcttta cagaccgcac tcgtagatga acaggccctg gaacagtcca
     3181 aaattcatct tgccagtacc cgtaagacct atcagcatac tttggagctt ttcaagaacg
     3241 gcgccgccag tcaggaacag ttggaccagg cggaactcac cctgagtcag gaagaaaagc
     3301 aagttgccca atccacccaa ttggccggta ttaaactgag caacgcccga ctggatctgg
     3361 accggacgaa agttcgcgcc acccgaaacg ggattattct tttttgcccg gcaaaagagg
     3421 gtatggccgt cacacccgga acagagctat gtcagatcgg agaactcagc aagctggtgg
     3481 tggaattgcc ggtggacgag attgatatca cccagatcaa gctggggcaa ccggctacaa
     3541 tcactcagga aggtttgacg gaactcaagt taaaagggac tgtaatcgcc attgctcccc
     3601 aggctgtgaa gaaccagaat gagaacgtgt ttccggtgaa gatccggatc gataactctt
     3661 cgggcatgct ccgctcggga atgtcggtcg ataccgagat tgtcaccaat gatctgaagg
     3721 atgtgctgac cgtgccgctc ttggcagtac ttgaggaaga acataaaggc aagactgaga
     3781 agtacctttt taaggtgatc gacggcaagg cagtcaagac taaaatcact gccggattta
     3841 gcagtgacag tgaactggag gttttgggcg gaatcaagga aggcgacacc tacgtttccg
     3901 gagactacga aaccatcctg catctcaagg ataaaaccgc ggtccggcaa agggtgttga
     3961 aaccgcaagc gcagcccaag gcgaaccagt aaccggcgag agaccctaca gggggttata
     4021 tgatgattag aattcaaaat ttgaacaaga tatataagaa cggctccttg gaagttcaag
     4081 cgctttttga tatcaacctg gaagtatccg aaggggagtt cgtggcgatc atgggacctt
     4141 ccggttccgg taagtcgacg ctgatgaaca ttttaggttg cctcgaccgg ccaaccaacg
     4201 gctcttatca actgaaaggc gtggaggtcg gcgggcttaa cgacgatcaa ttggcggaga
     4261 tccggaacaa acggatcggt ttcatctttc agactttcaa cctgttgccc cggagcaccg
     4321 ccctgcaaaa tgtggagctg ccgatggttt acgccggggt tcatagaggg gagcgacatc
     4381 aaaaagccat ggatgccttg aaaatggtgg gcctcgccga gcgctgccac caccggccca
     4441 atgagatgtc cggcggtcag cggcaacggg tggccatcgc ccgggcgctg atcaacaatc
     4501 cctccatcat tttggcggac gaacccaccg ggaacctgga tagcgtctcc ggacgggaga
     4561 ttatgatgat tttgcaggcc ctcaatgaac agggcatcac cattattctg gtaactcatg
     4621 ataatgagat cggccgccat gccaaacgaa tcgtgcgatt ccgcgacggc cggatcgtct
     4681 ccgacgaacc ggtgagcgat cggatcgaga ttggaagtga gagccatgat gccgcagggt
     4741 gaatggatta agatggcctg taccaatcta tggaacaata agctccgctc ttttttgacg
     4801 ttactcggca ttgccatcgg aatcggggca atgatcgcct tgattggtat aggatatggc
     4861 tttaaggctt ccatgttgaa acagtttgaa agcattggaa ccaacaccat catggtcttt
     4921 ccaagttctt atcaagagac cggaaagaaa atgaagacct taaaggagcg ggatcgccgg
     4981 gtgatccagt ccgtctgcgg aaacgatgcg gcggtggttc cattcatctt tgataatggc
     5041 cgcgccatca gttatcgtgg aaaaagcact tcgcccggga taaccggaac cagtggagac
     5101 tttaatgccg tagagagcct aaaacttaag tacggccgtt tcctgaatca atccgatgtg
     5161 gccgcaggta gccgggtggt agtgctcgac tggggtgtgg cggagaagct atttggatca
     5221 atgaatccgg tgggaaagaa tgtccaactt tacaaccggt catttcgggt tatcggttct
     5281 ttggagaaac gggattccag cgtcagtatg ggctcgggtt caggggggaa aaatctctat
     5341 ttaccagtca ccaccctgca acggatgatg aatttttatg agtattacgg tctttatgtc
     5401 agcgtgtccg atatgtcaaa gactgatacg gtaatggaga agctcagggg cattttggct
     5461 atccgttacg gttccgccga ctattttcaa attttcagca cgaaacaatt gcttcagatg
     5521 attacgaccg tcatgggagt cattacgacc ttgctgggag ccatcggagc cattgctctc
     5581 gtcgtaggcg ggatcgggat catgaatatc atgttggtaa cagtggcgga gaggatacgg
     5641 gagatcggcg tccgcaaagc aatcggcgcc aaaaagaaga atatcctgtg gcagtttttg
     5701 ttggaatcgg tctttctctg cctgcttgga ggcactctgg gcactggtat cggttttctc
     5761 gcgagtttta tcatttccaa gcttgcgccg ttgcaaccgg taattacccc gggtttaatc
     5821 gtgaccgcgt ttatctttgc cacggtggtc gggttgtcct ttggagtttt tcccgcctgg
     5881 aaagcggcaa aattggaccc gattgaagcc cttcgccatg agtaggggag gtggttcggc
     5941 atgcaaatca gtgaagcctt gtttatggct ctaaaccagc ttaaggcgaa taaaatgcga
     6001 tcatttctga cactattggg gattattgtc ggtatcgccg cggtcattgc cttaatggcc
     6061 gtaggaaacg gcgcggtcga gatgatctcc ggccaatttt ccgcagcagg gaccaatatg
     6121 gtttttatcc agtgggatta cggtaatgaa aataatgacg atttggttcc ccaattatta
     6181 caagataaag atttagccgc tatccgggcc ggcggtgatc tggcgagtaa tgtcgttcct
     6241 tataatcaaa ccgcttctga agtacgggcg gggcgcgaga ccctcgcggt cacaatcacc
     6301 gggatcaatg agggattttt ggaattacag ggattgaagc ttgcccgggg tcgttttttc
     6361 aacagtttcg aggtcacaag cgccaagagc gtctgcgtgt tgggttggga tttgcatgat
     6421 aagctgttta agaatagcga cggtcttggc aagaccatcc ggctgcgggg caagaagttt
     6481 ctggtcatcg ggatcatgga gaaaaaggat gatcgctcct ttgtcaacac cggcttaacc
     6541 gataatatgc ggctttatat accctataag acgttacagc ggatgtggaa cgtcaagggt
     6601 tacccggtaa ttatggcaca acccatcgac attaccatca ctaaggcgct ggtaggtcag
     6661 atcaaatgga tcattgcccg gatatacggg ccaaaaaata attttctcgt tcaaagcatg
     6721 gagcagatcc tggaacagac cctcagccgg atgcagatct tttcgatctt tctggccggt
     6781 ttgggaggca tctccctttt ggtcgggggg atcgggatca tgaacatcat gctggtctcg
     6841 gccaaggagc ggacccagga gatcggggtc cggaaagcct tgggcgccaa acgtaagcag
     6901 attttgctgc agttcctcat tgaagcgata gtgctctgcc tgatcggcgg gttcctcgga
     6961 attgctctgg gatttctcat cgccgtgggc atcaaggcaa ctaatctgat acctgctgcg
     7021 gtcagtctct cctcggtgct gcttgcggtg gggtgctcac tggcgatcgg tctctttttc
     7081 gggatttatc cggcctggaa agcggcgcaa ctcgatccga tcgagagctt gcggtacgaa
     7141 taactttgaa tgaatcaagt caccccttct tcatatcata agaaggggtt attttatgtc
     7201 ttgacaaaaa aagatttcta tattaaaatt taactatgtt aaacaatttg ttccgtttca
     7261 gtgggtatga atatccgaaa aagaactaga atagggaggt aacacaatga aaaaatggca
     7321 atgcaccgtt tgcggttata tctatgatcc ggagaagggc gatgggggcg gtgtccctcc
     7381 cggggtcgct tttgaggatc ttccggatga ttggacctgc ccggagtgtg gggcaggtaa
     7441 agacctattc gagccggtgg attgattaac cccccatcgg ggggggttcc acatccggcg
     7501 tcaaaggagt gtcgcggaat ggagactcat gaactcgctc cgaatgtatg gtggatcgga
     7561 gcgctggatc cgcaattacg ggtctttgac attattatgc gtgccgagca cggaaccagc
     7621 tataatgctt atatggtaaa gggatcccgg ggaattgcgg ttatcgagac ggtgaagagc
     7681 ggattctttc gggagtttat agatgaagta aagtcactcg ccgatccggc gcagattaag
     7741 tatatcatcc tcaatcatat ggaaccggat cattcgggca gcatcggcga actcttgaag
     7801 ttggcgcccg aagcccaagt ggtggtctct aggaccggag agcattttat caaacatatc
     7861 ctgaactatc agccgaaact gatgaaaatg ggcgacggcg accggattga ccttggcgat
     7921 aagcacctcc ggttcatcgc cgcccccttt ttacactggc cggatagtat gtttacctac
     7981 ctggaggagg atcaaatcct ctttgattgt gattttctcg gggcccatta tgccgatccg
     8041 atgctttttg gagatcagga tgaaggattt ttggaagatt ttaagtatta ttatgatcat
     8101 atcatgcggc cgttcaagcc atatgtgatc caagccctgg acaagctggc tgcgatgccg
     8161 cttgcgatgg tcgctccggg acacggaccg gtaatccgcc atgatatccc caagtatctc
     8221 ggttactacc gggaatggag catggccaat cgtgaggaag cgaaagtggt catcgcctat
     8281 gtcagcgcct acggcaacac cgctaaactt gcactcaaac tggctgaggg tattcaggcg
     8341 agtggagtgc cggccgagat ttataatctc gaacaggtgg ctcccgggga actggtggac
     8401 aaaatagagg ccgccaaggg cttactggtc ggttcgccca ccatcaacgg agatgcggtc
     8461 aagcccgctc acgatctact gagcagtctg gccacgatca gtctcaaggg aaaactgggt
     8521 gcggccttcg gttcgtatgg ttggagcggg gaagcggtat ccattcttga atccaggatg
     8581 gcggggatca aatttaaggt gatgaactct acggtccggc cggtgctaat acctaccgag
     8641 gcggacctgg ccaatgcggt ggagtttgga aaggcgttcg gcgctgcggt aaggggttag
     8701 ccccctacac gaatagcgat atcttcgatt ccatggcgtt ttgaatgaag gtgtcgacac
     8761 cggccacttc caaccggtcg tattcgatga gttcctcacg acggatcccc agaagtttga
     8821 gggaagcttc acaggcgatg aaacggacct ttctttccat ggccatttga aagagatccc
     8881 gggcggtctg accgtccttg tcgcggatga gtttcttcat caaccggggt ccgatgccgc
     8941 agaaattcat cttggaaagc ccgatccgct caatgcccag cggcatcagc cgtttgaaga
     9001 ggttttcaag caggttgtcc ccgctgagaa accttcgtcg caacagattt atgccccaaa
     9061 aggtgaagaa gaccgtcacc tccatgcccc ggccggcagc gccgttggca agcgtgaaag
     9121 cagccatcgc cttatcaaaa tcgccgctga agaggatcat gctgaattta tcgggtgggg
     9181 ttcccggatc tttctgggcc ggggtttgct ttggagtcat tcgtgcctcc gtcgtggttt
     9241 aaagttttta aaagccgata tccctaggat aagccggctg cgattagttt aaactggtag
     9301 ttttgcgaaa taagaaccta cactaactaa cgaattgagc tgaatgaatg ataccatatc
     9361 atgagttgac actagcggat aaagcgagtt ttgaccggat tttgagcgta ttgcaaccgg
     9421 agtcctcgga tttgactttt accaatcttt tcatgtggca atacaattat aatctgcact
     9481 ctttatacct tcaggatctg gacttttggg tgctgctcgc cagacccacc caatggaaag
     9541 ccttttacct gccgccgctg ggcgattgga ccgataaccg taagcttggc gcggtactgc
     9601 aaaagatgcg ggactgggcc gaagcggatg gctttgaatt ccggatgcgg cgcattcccg
     9661 aacccttagc ccaagcgcta gagcggatcg atccggctct ggcgctgact cctgaaccaa
     9721 atacctcgga ctacgtttac cgggctcagg atttgatcac cctggcgggc cggaagtacc
     9781 atggcaaacg gaatcacttg aatcagtttc agcgcaaata tgcctgggag taccaacgga
     9841 tgactccgga gattgtagag gaatgcctgc agtcggataa ggaatggttc aatatctgcg
     9901 gcgctttgga aagggacggc gccaccgatg aggagaaggc aatggcgctg gtgatgcaga
     9961 attatgcggc gctgggactg accggcggag tgattcggat taacggccgg attgaagcgg
    10021 tctccgtcgg tgagcggcta acctccaata tggctgtgat ccatatcgaa aaggccaaca
    10081 ccgaatttga cggtacctat acaggcatca accagcaatt tgcggcggac gcctggaagg
    10141 atttcgagtt catcaaccgc gaggaggatt tgggactgga gggtttacgg aaggccaagg
    10201 agtcctatca tcccgagaag atggtggaga agttcggggt aaagtattag ttattcgtga
    10261 ttcgtgattg gttatatttt aaatattcga atgattatga agcgttgccg gattttccgg
    10321 cagcgtttat ttttttgtca aaatgaaact gccgttcttt tgcgtttagt aatttttcaa
    10381 aataaattgg cggagtttga agtttattgg caggaaaata gaggaagctg aagaatataa
    10441 caacaggttt tacatggagg atattggaag ttgagtagaa acaaaaagca attaaaaagg
    10501 aagaaacaat aaaaccggcc tgtgtagggg ctggttttta ttttgtgacc gttaaaatcc
    10561 gggaaaattc gacaaagatt ttgataaccg atcattatat atccattttg ttcattaacg
    10621 gatagtagag aacaatccag tcatcacact aaagtaacta cgatttctaa aaaaaggtgg
    10681 tatgtagaaa tgggaaccaa taaatatttt aagactttga aaataataac aatcagtttt
    10741 atattattta cactcattgc tatttcagac ttaattccaa atgttaaggg ttctatacga
    10801 aactatactt ttagatttac tgatagtact cagggtagta ctaattgtaa taccatgagc
    10861 gcatattttc cttcagattg ttcccaaggt acacacgact ttgcgggttt aggttggaga
    10921 gcatccagat acgttggtgg aaaccttaca gtctgtcctt atcgaattac aatgaattat
    10981 agtgtacatt ttgatggttc tttcccttca aattcaattg ctccatggtt tagaataagc
    11041 cgttgtgatg caaatggtaa tattagtcaa tatggagatg ttatttatta ttctgatgga
    11101 aatggaatta cgaattatag tgtttcgcta gagccttatc aaggcattta tgtagaaatg
    11161 aattcaggag aaaattggac cagactaggt cttaataaga tatatcaagg gtacacatgt
    11221 aacttttccg tcaactacaa cattgatgat gttaaaccac tagccccaac tggttttggg
    11281 attcaagaca atcaacaata tagttataac gacgtgattt atacaaagac aaatccctta
    11341 agttttaaat ggaacaaggg ggtcgatcaa gggaatcccg tatcagcagt cgacggttat
    11401 tatctctatt atggcaatga cagccaatgg attgatgagc cacaaacagt ttttagcgat
    11461 aacaaaggct ggttttcaaa tgaaggtaaa tatacggttc agttaaaagt caaagataat
    11521 gccggtttat taagtgatct tgccagttta acctttacag tagatcgaac cataaatcaa
    11581 gttacattcc ctagt
//
LOCUS       DASVMB010000188         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_540044,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000188 DASVMB010000000
VERSION     DASVMB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540044"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF531_05685"
     CDS             <1..1325
                     /locus_tag="VF531_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713493.1"
                     /translation="AMYNPVKKLMSDIQKRKGIDFKTMGNEMAILSRVFDSMIKQEDS
                     LSNTLEKSRQALKNKFLTDLFKGGLEEESRDRQYEGEFPYPYFICAVIAIDKYDGFSE
                     KYSNDQQYYMKHLILKTCCEVANDKYKITGVLYEKSKIALIINCEEYDQESNRTTLSS
                     NFGALQNEVAKVLSNPITIGVGSCYKNIENISLSFNEAQEALKSRIVRGSGSIIFHSC
                     NEIENKYYYPYRMEKHILNLLQLGSGEEVILALKDLIEEIKRRKGISPDNVEQIFIQL
                     VGNTVKYLVELNINIRNIFGSGSNVFHELSTKENLEEIYTWLTGFYSGIIQFMSNAAA
                     LKTSPADIVMDYIHKNFKLNIDTTAIADNTGVGYSSVGRIIRSKTGKNVLEYINGLRI
                     EEAKRLLKSTTMNIKDIAVNVGYNNDQSFARFFKKYEGLTPGEFRNLG"
     gene            complement(1333..1797)
                     /locus_tag="VF531_05690"
     CDS             complement(1333..1797)
                     /locus_tag="VF531_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7713494.1"
                     /translation="MLAIPKKTLIDLQVNGETRTVVVRPADTLLDVLREQLGFTGAKP
                     GCRNGDCGACTILMDGWPIKSCLTLAVEAIGHQVTTIEGLQDTPIQKAFLDKWAFQCG
                     YCTSGFIIVCHALAQIHPGAGDELIEEWLQSNLCRCTCYEEIQAAVKSVLLV"
     gene            complement(1797..2633)
                     /locus_tag="VF531_05695"
     CDS             complement(1797..2633)
                     /locus_tag="VF531_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002161223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7713495.1"
                     /translation="MIPFDFEYYRPETLQEAVELYQNLNSRGLAPLYYGGGTEIISMA
                     RLSTVHTGAVIDIKAIPECNTLEIKDNRLITGAAVTLTLVMESGLFPLLAKSGGRVAD
                     HTIRGKITLGGNLCGKIIYREAVLPFLLAESEVIITGPGGERTINIQDVFQQTMQLNP
                     GELLVQVVTGKEYLSLPHDEVKKTRIDKIDYPLVSVAALKKDGQLRVAVSGLCAFPFR
                     STMIEAEINNSKLGFKDRVNNTLRHLPAPILSDIQASAEYREFVFKNTLLKIIRELNE
                     VA"
     gene            complement(3059..3388)
                     /locus_tag="VF531_05700"
     CDS             complement(3059..3388)
                     /locus_tag="VF531_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7713496.1"
                     /translation="MNFKIRINPLAVSDVLEIKASIAEDNPGAAAKIGNSIYSQIAEL
                     ADFPEMGANLRTKINMKTDYRYLVCGSYLIFYKIEGEFISVYRILNGVRDYLAILFDE
                     ESTGNQN"
     gene            complement(3391..3639)
                     /locus_tag="VF531_05705"
     CDS             complement(3391..3639)
                     /locus_tag="VF531_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7713497.1"
                     /translation="MPNIKPVSDLRNYNEVLRSCQVGEPVFLTKNGRGRYVLLDMREY
                     EKQQAVIKLLSKLSEAEETIKTGEEWKSLDDLKKTLGV"
     gene            complement(3862..>4671)
                     /locus_tag="VF531_05710"
     CDS             complement(3862..>4671)
                     /locus_tag="VF531_05710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7713498.1"
                     /translation="LGGVYLGIRSALARFPARPIRVLYAGTGPFATLALPLTTVFSSA
                     EVRFTMLEINPASVNCLKRVIAAFDAWDFVTEIICVDATRYRDDPDQPFQMIVSETMQ
                     NALQKEPQVAITLNLVPQMAGAGILIPENIEVEAVLLNPRLSQQRMLTGIAGDYYHSL
                     GKIFELNKETARAYARSVDLNSAGSFFDEVELAIPARRAPGYTQLCLFTAIRVFGTVE
                     LTPWQSSLTMPKQIMNLNHAEQPVEKISFQYKIDRQPGVEYHCGTSIPAAG"
BASE COUNT         1327 a         1110 c         1038 g         1196 t
ORIGIN      
        1 gggcaatgta taacccggtc aaaaagctga tgtcggatat tcaaaagcgt aaaggcattg
       61 attttaaaac catgggcaat gagatggcca tcctgtccag ggtatttgat tccatgatca
      121 aacaagagga tagcttatcc aacacactag aaaaaagcag gcaggcatta aaaaataaat
      181 ttctgaccga tcttttcaaa gggggcctgg aggaggaaag ccgggacagg caatatgagg
      241 gggaattccc atatccatat ttcatatgtg cagtgattgc cattgacaag tatgatggat
      301 tcagcgagaa atactccaat gaccagcagt attatatgaa gcatctgatc ctgaaaacct
      361 gctgcgaagt tgcaaacgat aaatacaaga taacaggcgt actctatgaa aaaagtaaaa
      421 ttgcactcat catcaattgt gaggaatatg atcaggaatc gaaccggact actctgagtt
      481 caaacttcgg cgccttgcag aatgaagtgg ctaaagtcct cagcaaccca attaccattg
      541 gcgtaggaag ttgttataaa aatattgaga acatcagcct ttcctttaac gaagcgcagg
      601 aagcgctgaa aagccggata gtgcgaggca gcgggagtat tatctttcat agctgcaatg
      661 aaatcgaaaa taaatattac tacccatacc gtatggaaaa acatattctc aatcttttac
      721 agcttggctc cggggaagaa gttattctgg cattaaagga tttgatcgaa gagatcaagc
      781 ggaggaaggg aatatctccc gacaatgttg aacagatttt tatccaactg gtgggcaata
      841 ccgtcaaata cctggtggag ctgaatatta atatcagaaa tattttcggt agcggttcca
      901 atgtttttca cgagctatcc acgaaggaaa atcttgaaga aatttatacc tggctcaccg
      961 ggttttattc gggcatcatc caattcatgt caaacgctgc cgctctgaaa acatccccgg
     1021 cagacatcgt catggattat attcacaaaa acttcaaact caacatcgat accaccgcta
     1081 tcgccgataa taccggtgtc ggttattcat cggtaggcag gattatccga agcaaaaccg
     1141 gtaaaaatgt tctggaatat atcaacggct taagaataga ggaggcaaaa cgcctgctta
     1201 aaagcaccac catgaatatc aaggatatag cggtaaacgt cggttataac aacgatcaaa
     1261 gctttgcccg ctttttcaaa aagtatgaag gtctgacacc cggtgagttc agaaatttgg
     1321 gatgaacgac tgtcagacaa gcaaaaccga tttcaccgcc gcctggatct cctcataaca
     1381 ggtacaacgg caaagattgg actgcagcca ttcttcgatg agctcgtcac cggcgccggg
     1441 atgaatctga gccagcgcat ggcagacgat tataaacccg gaagtacagt aaccgcactg
     1501 aaaagcccat ttatccagaa aagctttttg gatgggggtg tcttgcaacc cttctatcgt
     1561 ggtcacttga tggccaatcg cttccaccgc caaagtcagg caagacttga tgggccaacc
     1621 gtccatcaat atggtgcaag ccccgcaatc cccgtttctg cacccgggtt tagcgccagt
     1681 gaacccaagt tgctcccgaa gaacatccaa caaggtatcg gcgggacgaa ccaccacagt
     1741 tcgagtttcg ccgtttactt ggagatcgat taaagttttc ttcgggattg ccagcatcaa
     1801 gccacctcgt ttaattcccg gatgattttc agtaatgtat ttttaaagac aaactcccgg
     1861 tactcggctg atgcttgaat atcgcttaaa atcggagccg ggagatggcg tagcgtatta
     1921 ttgactcgat ctttaaagcc taatttggaa ttattaattt cagcttcaat catggttgac
     1981 cggaagggaa aagcgcacaa tccgctgacc gccacccgga gttggccatc tttctttaaa
     2041 gcggcaacgc tgactaaggg gtaatcgatc ttatcaatcc gggttttttt gacttcatca
     2101 tggggcaacg acagatattc tttgccggta actacctgca ccaacaattc acccgggttt
     2161 aactgcatgg tttgttgaaa aacatcttga atgttgattg ttctttcccc tccgggaccg
     2221 gtgatgatca cttcactttc tgccagcaga aatggcaaca cggcctcccg gtaaatgatt
     2281 ttaccgcaaa gatttccccc aagggttatt ttaccccgga tagtatgatc ggccaccctc
     2341 ccccctgact tggccaataa cggaaaaaga cccgactcca tgactagggt cagagttacc
     2401 gccgccccgg ttattaaccg attatctttt atttccaagg tattgcactc tggaatcgcc
     2461 ttaatatcaa tcacagcacc ggtatgaacc gtacttaacc tggccatgct gataatctcg
     2521 gtccctccac catagtaaag cggggctaat cctctggaat ttaaattttg ataaagttca
     2581 accgcttcct gaagagtctc gggccggtaa tattcgaaat caaaggggat cattaccgct
     2641 acctcctttg gctttccata tccgttccgg aaccaagggt cttggggaca ttggtagctc
     2701 cttgggaaaa ttacgttttt ttaatatacc ccttttcgcg cagtttatat tccgtcaaat
     2761 ccgttccaac agcaaccgga atccgagtga atccgcttta agagcaccag aaatagcact
     2821 tcggggcaat gacccttctt gaaacaggga atttctgtga ctgaactcat ccgtaaatca
     2881 atggatcatt atttaaaaca gatcaacgga gagataaaca tgaactgatt gaaagggcca
     2941 agggagcggc ggggaaatat cataccggtt tgaaagattt ggctgaaaat catgaccggt
     3001 atcttacgga ggatttgcag aatagaaaca tacggcctgg agcaactagc tacaaattct
     3061 aattctgatt tccagttgat tcctcatcaa aaagaatggc tagataatct ctgacgccgt
     3121 ttaaaatacg gtaaaccgat ataaattctc cttcaatctt ataaaaaatc agataactgc
     3181 cgcatacaag atagcgataa tccgttttca tattgatttt cgttcttaaa ttggctccca
     3241 tttccgggaa atcagccaac tcggctattt gggaataaat agagtttcca atctttgcgg
     3301 cagccccggg attgtcctcg gcaatggatg cttttatttc cagaacatca gaaaccgcca
     3361 gcggattaat tctgattttg aaattcaccc tcaaaccccc aatgttttct tgaggtcatc
     3421 aagtgatttc cattcctctc cggtcttgat cgtctcttcc gcttcggaaa gctttgacag
     3481 aagtttaatc accgcctgtt gtttttcgta ctcccgcata tccaaaagaa catagcggcc
     3541 tctgccgttt ttagtcaaaa ataccggttc cccaacttgg caggaacgga gcacctcgtt
     3601 ataatttctc aaatcggaaa ccggcttaat attaggcatc acaaatacct ccaggattta
     3661 ttgcttttat tgtagcctaa tatttcgtaa aatacaacat catattttac agcaaaatga
     3721 tgattgttat agtttatgtc aatcacccgg ctgcgtggag ctgatgtatg gccgttccat
     3781 ccggtatttg gtgccggacg cggtccggga gtatattgcc gccaaaaaac tttaccggaa
     3841 cttctgaaaa catcaaacgg atcaaccggc cgctggaata ctggtaccac aatggtactc
     3901 aacccccggc tgtcgatcga ttttatattg gaaactgatc ttctcgaccg gttgctctgc
     3961 gtggtttaga ttcatgatct gcttgggcat ggtcagcgaa gactgccagg gcgttaattc
     4021 taccgtgccg aaaacccgaa tggcggtaaa tagacacaat tgcgtatacc ccggagcgcg
     4081 ccgggccgga atcgccagtt ccacctcatc aaagaaagat ccggcgctgt ttagatcgac
     4141 tgaacgcgcg taagcgcggg ccgtttcttt gttaagctca aatatcttcc ccaatgaatg
     4201 ataataatcg ccggcaatgc cggtcaacat ccgttgttga ctcaacctgg gattgagcag
     4261 caccgcctcg acctcaatat tctccgggat taaaatgccc gccccagcca tttggggaac
     4321 caggttcaaa gtgattgcca cctggggttc tttttgcaag gcgttttgca tcgtttcact
     4381 gacgatcatt tgaaacggct ggtccggatc atcccggtac cgggtggcgt cgacgcaaat
     4441 aatctccgtt acgaagtccc aggcatcaaa agcggcgatt accctcttca ggcagttgac
     4501 actggccgga ttgatctcca gcatggtaaa tctgacctcc gcgctggaga agacggtggt
     4561 taaaggcagg gccaacgtgg cgaagggacc ggttccggcg tagagcaccc ggatcggtct
     4621 ggccggaaac ctggccaagg cagaccggat gcccagataa acaccgccca g
//
LOCUS       DASVMB010000189         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_687960,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000189 DASVMB010000000
VERSION     DASVMB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687960"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF531_05715"
     CDS             complement(<1..259)
                     /locus_tag="VF531_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713499.1"
                     /translation="MSDLLINGFNNLITKKRFRARRASSYDVTGGNADSWSIAPGETK
                     VLADLKGPGIISHIWFTVGSPDKLYLRKILLKIYWDDEASPS"
     gene            complement(374..2935)
                     /locus_tag="VF531_05720"
     CDS             complement(374..2935)
                     /locus_tag="VF531_05720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEX7713500.1"
                     /translation="MDLKVGFPAGMPLGGIGTGLIELMTNGKPGALLINNNWAQPLTD
                     VDGCFFAVRIEQASGETIFRLLQSESRSKFQGVTHVHYSGLFPVAKAEYLAPEIPMRM
                     EVKAFSPVVPHQVEKSNIPGVLFRFKFTNLYDEKIKLKLFVSWENLLGCGGALEGPVD
                     SDRTGNFVVPFSDRRHQGLVFKTSSATKKAYPNALGEYALLTPIDAQTRFTSYVYNAV
                     DEMDQVLNAFSSDQAFGADNLNGVEGAIHPAGAIGIGVELGAYQECEIPVVFAWYTPY
                     HRVMEGRNEKAQNILKQYAPLYEENTGLTTTVKLKDYGHFYQNRFATAVAAGAYLLEN
                     GDPLLRDTTELHAFINSSGLPQWLKAKVINDSNPLTANTVLTRAGILATLEASRGMGG
                     ALGTMDQRLVAHAAYQLFYPDLNRTELQLFAGIQAEDGHITHFCGNFYESIGSSQVSY
                     GDTTWPDLSCSFIIQCYRDLMNTGDYEFFQRMLPHIMRAYQWLKTADRDGDFIPEGGS
                     SWDTEHQAGLFVYTGTVWLATLRVMEQIATLEKDEALKGDVKKRFEKAQENLIRCLWN
                     GAYFNCGYDPGTGAVSTEIFPGQFAGEWVVRLLGLPGILPSAMIRTAIGNIYKLAGNK
                     DLYRMIPIRTRKNGSRVGRNLDEQAWPQYTMVFLDLVAVYLGLLDEAIVDIKRFDEAV
                     CDRTRAPWTTTLWHDCRTGMPCGGRLDRYMNCTASWFILNALTGFHHDAIAREMILGP
                     AAGMNQPGEFPLKLSPFKLPLVSAGYWAMLTVAADGNQVTLDIHFEKLFDARVELAGV
                     KLRGDCRSVSCRVNGREIGIRRQAGTEYTGIQFDNPGKVSLLQGDQITVRYSLAG"
     gene            complement(2963..>3299)
                     /locus_tag="VF531_05725"
     CDS             complement(2963..>3299)
                     /locus_tag="VF531_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713501.1"
                     /translation="YGLYLILFAMTGAVNAATMVGSFNLLMSVIPSTDRFVFLSMNSI
                     IMGIAGFLAPNIGAYFIDLLSDIRLPLSVFHTPPIRQLFIFGSLGSLVLALTFGKFVK
                     PLPESGERC"
BASE COUNT          822 a          913 c          791 g          773 t
ORIGIN      
        1 ctgacgggct tgcttcatca tcccagtata ttttgagtag aatctttctc aaatacagtt
       61 tgtcgggaga acctaccgta aaccagatat ggctgatgat ccccggcccc ttcaaatccg
      121 ccagaacttt tgtttcaccg ggagctatcg accaggaatc agcgttccct cctgtaacat
      181 cgtaactgct ggctctcctc gcccgaaatc tcttcttggt gataagattg ttgaagccgt
      241 tgataagcaa gtcactcatt gattacatcc ccccctgtca attacaaaat tttaagcgag
      301 gtagcggtat atctgatttc taaagaccct tccgaatctt gcaggaaatt gatatcggat
      361 acttttgtta tgatcaaccc gccaaagaat agcgaacagt tatctgatcc ccctgcagta
      421 gactaacttt acccggattg tcaaattgaa tcccggtata ttctgttccg gcttgccggc
      481 ggatcccgat ctccctgccg ttcacacggc acgataccga acggcaatcg ccgcgcagtt
      541 tgacccctgc cagctcgacc cgggcatcaa agagcttttc aaaatgaatg tccagggtaa
      601 cctgattacc atccgcggcc acagtcagca ttgcccagta acccgcgctc accagcggca
      661 gcttaaacgg agacagctta agcggaaact ctcccggttg gttcatcccg gcggcgggtc
      721 ccagaatcat ctcccgggca attgcatcat gatggaatcc agtcagtgcg ttgagaataa
      781 accaggaagc tgtgcagttc atataacggt cgagcctgcc gccacagggc atccctgtcc
      841 ggcagtcatg ccagagggtg gtggtccagg gagcccgagt ccgatcgcaa acggcttcat
      901 caaaacgttt gatatccact atagcttcgt ctaacaaccc cagataaacg gcgaccagat
      961 ccaggaaaac catggtatac tgcggccagg cttgttcatc cagatttcgc cccacccggc
     1021 tgccgttttt gcgggtccgt atcggtatca tccggtataa atccttattg ccggctaatt
     1081 tgtatatatt accgattgcg gttcgaatca tcgcagacgg caggatgccc ggcagaccca
     1141 atagccggac cacccattcc ccggcgaact gccccggaaa gatctcggtg gagacggcac
     1201 cggtaccggg atcatagccg cagttgaagt aggcaccgtt ccataaacaa cgaatcaagt
     1261 tttcctgggc cttttcgaag cgttttttca catcaccctt caaggcctca tccttttcga
     1321 gggttgcgat ctgttccatc acccggagtg ttgccagcca gacagttccg gtgtacacga
     1381 ataagccggc ctgatgctcc gtatcccagc tggaaccgcc ttccggaata aagtccccgt
     1441 cgcgatccgc cgttttcagc cattggtatg cccgcatgat atgggggagc atccgctgaa
     1501 aaaattcata atccccggta ttcatcaggt cccggtagca ttggataata aacgagcagg
     1561 agagatcggg ccaggtggta tccccgtaac tgacttgact gctgccgata ctctcataaa
     1621 aattaccgca aaaatgggtg atatggccgt cttccgcttg gatacccgca aatagctgca
     1681 gctcggtacg gttaagatcc gggtagaaca gttgatacgc ggcatgagcc accagccttt
     1741 gatccattgt tcccaaggct ccgcccatcc cgcggctggc ttcgagtgtg gccaggatgc
     1801 ctgcccgggt caggaccgta ttggcggtga gcgggttgga gtcgttgatt acctttgcct
     1861 tgagccattg gggaagcccg gaagagttga taaaggcatg cagctccgtg gtatctctta
     1921 ataacggatc cccattctcc agaagataag caccggccgc gacggctgtt gcaaaccggt
     1981 tttgatagaa atggccgtaa tctttcaatt tgactgtagt agtcaagccg gtgttttctt
     2041 catatagagg cgcatattgt ttcaagatat tttgcgcctt ctcgttccgg ccttccatga
     2101 cccggtggta cggagtatac caggcgaaga ccaccgggat ttcgcactcc tgataggccc
     2161 cgagttcgac gccgattcca atagcccccg caggatggat cgctccttcg acaccgttca
     2221 gattatcggc accgaaagct tgatcggacg aaaaagcatt taaaacctga tccatctcat
     2281 cgaccgcatt ataaacatac gacgtgaacc tcgtttgagc atcgatcggg gtcaataagg
     2341 catattcacc cagggcgttg ggatacgctt tctttgtggc ggatgaagtt ttaaaaacca
     2401 gtccctgatg ccgtcggtca ctgaaaggca caacaaaatt ccccgtccgg tcggagtcca
     2461 ccggaccctc aagcgctcca ccacaaccca acagattttc ccaggaaaca aagagcttca
     2521 gtttgatctt ttcatcatat aaatttgtaa acttaaaccg gaataacacg ccgggaatat
     2581 ttgatttttc aacctgatgc ggcacgacgg ggctgaaagc cttaacctcc atccgcattg
     2641 ggatctccgg ggccagatac tcggccttag ccaccgggaa cagtccggag taatgcacat
     2701 gggtcacgcc ttgaaatttt gagcggcttt cactctgaag gagccggaaa atcgtttccc
     2761 cgcttgcctg ttcaatccgg accgcaaaaa agcagccatc tacatcggtc aaaggttgcg
     2821 cccagttgtt attgatgaga agtgcacccg gcttgccgtt cgtcatcagt tctatcaaac
     2881 cggtaccgat tccacccaac ggcataccgg cgggaaaccc cactttgaga tccatcactc
     2941 gtcctccata aattaatgaa atctagcatc tttccccgga ttctggcagc ggcttgacga
     3001 attttccgaa agtcagtgcc agcacgagcg agcccaaact tccgaagata aagagctgcc
     3061 gaatcggggg cgtatgaaaa acggaaagcg gcaaccttat atcggaaagc agatcgatga
     3121 aataagctcc gatgttcggt gcgagaaaac cggcaatccc catgatgata ctgttcattg
     3181 ataagaaaac aaatcgatcg gtcgacggga tcaccgacat cagcaagtta aagctcccaa
     3241 ccattgtcgc ggcattgact gcgccggtca tcgcgaacag aatcaggtaa agtccgtag
//
LOCUS       DASVMB010000190         6160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_100291,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000190 DASVMB010000000
VERSION     DASVMB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100291"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF531_05730"
     CDS             complement(<1..234)
                     /locus_tag="VF531_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX7713502.1"
                     /translation="MSKTGKILLAALIIVVVILWAISGKYLDWLWFKTMGPTSVFWVS
                     LLTGPLTKLIIGLVAFAFFIVNFLIAIRAFDRIK"
     gene            388..1224
                     /gene="pyrF"
                     /locus_tag="VF531_05735"
     CDS             388..1224
                     /gene="pyrF"
                     /locus_tag="VF531_05735"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7713503.1"
                     /translation="MACFIDLLEGRWKQGHFLCVGLDTDYRQVPDDLRGADPAEGIFR
                     FNCAIIEATAAYCCAYKPNSAFYEGYGSAGIEALRRTNLFIREHYPEHPIILDAKRAD
                     IGNTNLGYVKACFEVLAADAITVHPYLGQEALKPFLDDPEHGVIVLCHTSNPGAAEFQ
                     ELPVGGIPLYQVVAERVARYWNRLGNCGLVVGATYPEQLAVVRGLIGDMPLLIPGIGA
                     QGGDVRLTVRNGLNSNGGGIIVNASRSVIYASSGSDFAVAAAREAARLDRELREAAME
                     VK"
     gene            1221..2156
                     /locus_tag="VF531_05740"
     CDS             1221..2156
                     /locus_tag="VF531_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HEX7713504.1"
                     /translation="MKQPRSRMIFGFVLWILSLYGYGTLAQNQTVTITILHSNDFHGY
                     NFELLAKRATLIKRVRATNPSPVLLLDAGDVFTRGRYARRFYGELEFAVMNGLHYDAL
                     TLGNNEFKATGNSSALQVLQSRIKQAAFPVLCGNVVDPNTGELISGVRPYIIKDIAGI
                     RCGILGVTARRSAGYRQLKGWKVLDPLVTAEGIAKELSGKVDLTIALTHIGVAEDRIL
                     AAALGMPAVIVGGDSHTILMEPLWVNGHPIVQAGDSGYFLGKLEITLEKTGQGWKLKD
                     ARSELLRLNDPQIEADPEVMQIIQEYLGKQLPQAA"
     gene            2397..3365
                     /gene="trpS"
                     /locus_tag="VF531_05745"
     CDS             2397..3365
                     /gene="trpS"
                     /locus_tag="VF531_05745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7713505.1"
                     /translation="MRVLSGIQPSGNLHLGNYFGMMKRMIEYQQNHQLFCFIVNMHAL
                     TSTFDAERLRRQTYNAACDFLALGLDPEKSVFWVQSDVPEVTELTWYLGNITGMGLLE
                     RCHSYKEKMAKGIPASHGLFSYPVLMAADILGYGAERVPVGKDQKQHVEVARDLAIRF
                     NNTYGETFVIPEPDIEDELATVPGLDGQKMSKSYGNTIEIFGDEASLKQKIMGLKTDS
                     TPVNEPKPIEGNILFALYSLFMDENGKAELKDRFLTPGLKYGDVKKELFATIWEHFEP
                     YRANRAALEEEYVIETLKKGATKARQVVTTYMDKARHQVGMDYFRQ"
     gene            3704..5053
                     /locus_tag="VF531_05750"
     CDS             3704..5053
                     /locus_tag="VF531_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX7713506.1"
                     /translation="MIPDFTKFGIAMPEVLLPNPRVDLANWSVIACDQFTSEPDYWEQ
                     VSRIVGQAPSTLKMIFPEIYLERGDAAERITTIQNTMREYLDQGILMPQPPGFIYLER
                     RTGSGVRRGLMVALDLEYYDYRPGSQSFIRATEGTVLERIPPRVRIRSGALLELPHIM
                     VLIDDPGRGVIEPLGMNRDRMKRIYHTELMMDGGEISGYRIDTPELLAGIWNGLAELG
                     APDSLERKYGPEGQYPFLFAVGDGNHSLATAKTVWEELKRSRPQVSNIPGYPARYALV
                     ELVNLHDPALQFEPIHRVIFGVNPEEFWDAMQHYYESLGAIVTREEFNDPETASIRIQ
                     SESSTKGQSIVYSSGLNSGIIHIISAPHLLEAGTLQMFLDHYKMQNPGVSIDYIHGSS
                     VVKQLTAESGNIGFYLPPFDKNTLFPAVISAGVLPRKTFSMGEAVEKRYYMECRRIL"
     gene            5560..5919
                     /locus_tag="VF531_05755"
     CDS             5560..5919
                     /locus_tag="VF531_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713507.1"
                     /translation="MQLSLNDHETLNRHEVSNRSELAAKGIDNVRVTRIGKAIKAMIA
                     EYNLALMHDLPLNNTYEGWAVLKQKLDELWEEVKLGETGPSRDVLRKEAAELGAATMR
                     FIVDLTAEESHRKETPL"
     gene            5961..>6160
                     /locus_tag="VF531_05760"
     CDS             5961..>6160
                     /locus_tag="VF531_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713508.1"
                     /translation="MGLFDHIPIATTLMHSSILGSGENTDEGDRQLRLILRICFSEPT
                     VMSNLIGKILGQLRNYFFKIGH"
BASE COUNT         1576 a         1392 c         1638 g         1554 t
ORIGIN      
        1 ttttatccga tcaaaggccc tgatcgcgat caggaaattg acgataaaaa acgcaaaagc
       61 cacgagaccg atgattagtt tggtcagggg cccggtcagc agggagaccc aaaagactga
      121 ggtaggaccc atggttttga accataacca atcaagatat tttccggaaa tggcccataa
      181 gatcacaacc acgataatta atgcggccag taatatttta ccggtcttac tcatttaaaa
      241 cccactcctt ttggatatac agttctttaa aacttcgctt cttccaaaaa aactcctgct
      301 ttgggaggaa gagtccaaac agaagcgaat atgagttaaa acatcgtaga gatttaaatt
      361 gagaatccgt attgggaggg atatggcgtg gcgtgcttta ttgatttatt ggaaggccgg
      421 tggaagcaag gccatttttt atgtgtggga ttggacacag attaccgtca agtacccgac
      481 gatctccggg gggctgatcc tgcggagggg atattccgtt ttaattgtgc gattatcgag
      541 gctactgccg catattgttg cgcatacaag ccgaacagcg ctttctatga gggctatggt
      601 tccgccggta ttgaggcact acgccgcacc aatcttttta tccgggagca ctatccggaa
      661 cacccgatca tcctcgatgc caagcgggcc gatatcggaa ataccaactt gggatatgtc
      721 aaagcctgtt tcgaggtatt ggctgcggac gccatcacgg ttcatcccta tctggggcag
      781 gaagcgctaa agccgtttct cgacgatccg gaacatgggg tgatcgttct ttgccacact
      841 tccaaccccg gagccgccga gtttcaggaa ttgccggtcg gcggaattcc tctctaccag
      901 gtggtggccg agcgggtagc ccgatattgg aatagattgg gtaattgcgg attggtggta
      961 ggcgcgactt atccggaaca attggctgtg gtccgaggct taatcgggga catgccgttg
     1021 ttgattccgg gaatcggtgc ccaaggcgga gatgtgcggc ttacggtcag gaacggtttg
     1081 aattcaaacg gcggcggcat tatcgtcaat gcctcgcgga gtgttatcta tgcctcaagc
     1141 gggtccgatt ttgccgtagc cgcggcccgt gaagccgccc gtctggatcg tgaattacga
     1201 gaggcagcga tggaagtcaa atgaaacagc caagatctcg aatgatattt ggattcgtcc
     1261 tgtggatatt gagtctgtac ggctatggga cactagccca aaatcagact gtcaccatca
     1321 ccatcttaca tagtaatgat tttcacggtt acaattttga gcttttagcc aaacgggcga
     1381 cactcatcaa aagagtccgg gcgacgaatc ctagtccggt attattgctt gacgccggag
     1441 atgtctttac ccgtggccga tatgctcggc gtttctatgg cgagctggag ttcgcggtga
     1501 tgaacggttt gcattatgat gcgttaaccc ttggtaataa cgagttcaaa gccaccggaa
     1561 acagctctgc cttacaggtg cttcaatccc ggatcaagca agccgctttt ccggttttgt
     1621 gcggcaatgt agtggacccg aataccggag aactgatttc cggggttcga ccctatatta
     1681 tcaaggatat tgccgggatc cgttgtggaa tcctgggggt gactgcccgg aggtcggccg
     1741 gttatcgaca attaaaagga tggaaagttt tggaccctct cgttactgcc gaaggaatcg
     1801 ctaaggaatt atcgggaaag gttgatttga ccatcgcctt aacccatatc ggagtagcgg
     1861 aagatcggat actcgcagcg gctttaggga tgccggcggt gattgtgggc ggggattccc
     1921 ataccatcct gatggaaccg ctatgggtaa acggccatcc cattgtacag gctggagatt
     1981 ccggttattt cctgggtaaa ctggagatta ccctggaaaa aacgggccag ggttggaagc
     2041 ttaaagatgc ccgtagtgaa ttgttgcgct taaatgatcc acagatcgaa gcggatccgg
     2101 aggtcatgca aattattcag gaatatttag gtaaacagct gcctcaagcg gcgtgatctc
     2161 ccggatctta cgcttgcaaa ttattgtaat atagcccata ataaatagtt aatatggaag
     2221 acagacatcg cgccccctgg ggcgcgcggc aacaggcaat agggaacagg aagcaggcta
     2281 aggaaagagc tttttagact tttctcttta cccgtttccc ttttgcccag tgcctgttgc
     2341 ctattgccat agatgatttt tcacttttca ttttttaatg caggggagtg ttgattgtgc
     2401 gcgttttatc gggaattcag ccatcgggta atttacattt gggtaattat ttcgggatga
     2461 tgaaacggat gatagaatac cagcagaatc atcaattgtt ctgtttcatc gttaatatgc
     2521 atgcgctgac atccaccttt gacgcggaac gtttacgccg acagacttat aatgcggcct
     2581 gtgactttct cgcgctgggt cttgatccgg agaaatcggt cttctgggtt caatcggatg
     2641 tccccgaagt taccgagttg acctggtatt taggaaatat caccggtatg ggtttgctgg
     2701 agaggtgcca ttcttataaa gaaaaaatgg ccaaaggaat tcccgccagt cacgggttat
     2761 tctcctatcc ggtgctaatg gcggccgata tcctgggata cggcgccgaa agggtgccgg
     2821 tgggcaaaga tcaaaaacaa catgtggaag tggcgcgcga tctggcaatt cgtttcaata
     2881 acacctatgg tgaaaccttc gtcattcccg aaccggatat cgaagatgaa ttggcaaccg
     2941 taccgggact ggatgggcaa aagatgtcga aatcgtacgg taataccatt gagatctttg
     3001 gggatgaggc ttcacttaaa cagaagatta tgggtttaaa gacggattct actccggtca
     3061 acgagccgaa accgatcgag ggcaatatct tgttcgcgct ttattcgctg ttcatggatg
     3121 aaaacggtaa agcggaactg aaggatcgct tcctgactcc gggtctcaag tacggggatg
     3181 tcaaaaagga gctttttgct acgatctggg agcattttga accctatcgc gccaaccggg
     3241 ctgcattgga agaagaatat gtaattgaga ccttgaaaaa aggcgcaacc aaggcccgcc
     3301 aggtagtaac gacatatatg gataaagccc gacaccaggt gggaatggac tattttaggc
     3361 aataggcaat aggcaacagg gaatagggaa ctcgcgcccc ttggggcgcg cggcaatagg
     3421 ctaaggaaag aggaaataaa aatgcgggga aaaccggatt cgggcctatt accctatcct
     3481 ttttaagtag agttgtatca caactcttaa gaaaacggtg tcaccgtttt caggggaccg
     3541 gtcatgtttt gctggttaat acctgtaacg cccagctgct tccctgttag ggatagagta
     3601 gataggccgg gatttagccc ggttacctct tgtcttttgc ctagtgcctg ttgccgtgcg
     3661 tcccgcagga cgcgatgcct tattttaaga agcaggggga ttcatgatac ccgattttac
     3721 gaaatttgga atagctatgc ccgaagtact cttgccgaat ccccgggtgg atttggctaa
     3781 ctggtcggta atcgcctgtg atcaatttac ttccgaaccc gattactggg agcaggtcag
     3841 ccggattgtg ggtcaggcgc cttcaacact aaaaatgatc tttcccgaga tctatcttga
     3901 acggggtgac gcggcggaac gaatcaccac gattcaaaat accatgcgag agtatctgga
     3961 tcagggcatt ctcatgccgc aaccgcccgg gtttatctac ctggagcgcc gaacgggctc
     4021 cggagtacga cgcggcctca tggttgcgct tgatttggaa tactacgatt atcggccggg
     4081 atcacagagt tttatccggg ccaccgaggg tacggtgttg gaacgcattc caccccgggt
     4141 caggatccga tccggagcac tgttagaatt gccccacatc atggtgctga ttgatgatcc
     4201 cggccgcggg gtcattgaac cgttaggaat gaaccgggac cggatgaaac ggatctatca
     4261 taccgaattg atgatggatg gcggcgagat ctccgggtac cggatcgaca cccccgaact
     4321 attggccgga atctggaatg gcctggccga gcttggtgcc ccggatagtc ttgagcggaa
     4381 gtacggtccg gagggccaat acccattcct tttcgcggtg ggtgatggta accattcgct
     4441 ggctactgcc aagaccgtct gggaggagtt aaagcgcagc cggccccagg tgtccaacat
     4501 accgggatat ccggcccgtt atgccctggt ggaattggtg aaccttcatg atcccgccct
     4561 gcaatttgaa ccgatacacc gggtgatctt cggtgtgaat ccggaagaat tttgggacgc
     4621 aatgcaacat tattacgaat cccttggtgc gattgtaacg agagaagaat tcaacgatcc
     4681 ggagacggcc tcaatccgga tccaatcgga aagttccacc aaaggtcagt ctatcgttta
     4741 ttccagcggt ctcaattccg gaatcatcca tatcatttct gccccccatc tgctggaagc
     4801 cgggacgctt cagatgtttc tcgatcatta caagatgcaa aatcccggcg tgtcaatcga
     4861 ttacattcat ggcagttccg tggtgaaaca attgacggct gaatccggca atatcggctt
     4921 ttatctcccg cctttcgata agaatacctt gtttcctgcg gtgatatccg ccggggtttt
     4981 gccacgtaaa actttttcaa tgggtgaggc ggttgagaaa cgatattaca tggaatgccg
     5041 ccggattctt taaaccttta ttcataacgc atgtttacat tattagcaat aacagagcag
     5101 tctctttagg ggagactgtt ttttattgcg ctataccggg gattattatc aattgcaata
     5161 aattgttttc agtggcaaaa gccgaaaaat accagataat ttcggcactt tcatcacaaa
     5221 atgcgacata aaaaagatac agtatcggtt atgataattc gagggacttg tgatgattgt
     5281 gaggcggttc ggatgattca ctggttgaaa tctttttggt acctaatccg gggtgagcaa
     5341 gcgggatacg agatattatt ttatcccaag gcggttaagt tacgccgaat tagcctgacc
     5401 gccaccggct tgagacctcc agcacctaat tctgggaacg gagagggagc aaaccggttt
     5461 aaaacccgcc cgagagcgga ataaccttta aagacaaccg ggtagttcgc tagttaggtc
     5521 ttggtttcat tcatccatca tttgaaggag gctgtcttga tgcaactgag tctaaatgac
     5581 catgagactc taaatagaca tgaggtttca aaccggagtg agctggcggc caaagggatc
     5641 gataatgtcc gggttacaag aatcggcaaa gccatcaagg cgatgatagc cgaatataat
     5701 ctggctctca tgcatgacct gccgctaaac aatacatatg aaggttgggc cgtgctcaaa
     5761 cagaaactgg atgaattgtg ggaagaagtg aaactgggag agaccggacc ttcccgggat
     5821 gttttacgaa aggaagcggc agagcttggt gccgcaacca tgcgtttcat cgtcgatctg
     5881 accgccgagg agagtcaccg gaaggagacc ccgttataaa tcaattcatt acgcaaacag
     5941 acccgccggc tgaaaagccg atgggtctgt tcgatcatat tccaattgcc actactttaa
     6001 tgcattcgtc gattttggga tcaggtgaaa atacggatga aggggatagg cagcttcgtt
     6061 tgattttgag aatctgtttt tcagaaccaa ccgtcatgag taatcttatc ggcaagattc
     6121 ttggccagct ccggaattac tttttcaaaa tcggccattt
//
LOCUS       DASVMB010000191        10544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_613654,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000191 DASVMB010000000
VERSION     DASVMB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613654"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1036)
                     /locus_tag="VF531_05765"
                     /pseudo
     CDS             complement(1..1036)
                     /locus_tag="VF531_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253416.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
     gene            complement(1107..3173)
                     /locus_tag="VF531_05770"
     CDS             complement(1107..3173)
                     /locus_tag="VF531_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65"
                     /protein_id="HEX7713509.1"
                     /translation="MTIDRYQLVSRHNPVLKKLDPRSPLSIGNGELAFTADITGMQTF
                     PEPYRDNMPLCTQSQWGWHTAPADDGRYFTHHDLSLQYFDVAGRRVGYAVSNEGQEEI
                     YDWLRLNPHRLHLGRIGLEMLTSDGNLAWPDDIQNIYQQLDLWRGILESRFTLGGVPV
                     NVKTCCHPTQGILAFSVVSELLPRGRLKVRLAFPYGSPDKTAADWSSDQKHNTVMIPM
                     DGVDFGLMRILDHNRYFVGIRHSNTGRLYRDGRNEFVMEPATGVSKLEFSVSFTPKPL
                     FEALPSFADIQAACEKHWKKFWLEGGAVTLAGSRDPQANELERRIILSQYLTAIQCTG
                     TMPPQETGLTCNSWYGKFHLEMHWWHAAHFPLWGRSRLLERSIWWYRTILPLAKGIAA
                     SQGYRGARWPKMVGPAGYDSPSPIGPLLIWQQPHPIYLAELCYRAHPSPGTLEIYRDI
                     VLETAEFMASYVQYDETNNRYVLGPPVIPAQENHRPETTLNPTFELEYWCFGLNTANQ
                     WRKRLGLDENETWREIASRLSALPVMDGFYLAHENCPATFSRFNLDHPSMLGALGVLP
                     GMKVDRGTMMKTLYKVLQEWRLEEMWGWDFPMIAMTAARVGRPDIAVAALLMDSPKNR
                     YLPNGHNCQGERKDLPLYLPGNGGLLTAVAMMAAGWDGCEFGDTPGFPKDGNWTVTWE
                     GLLPMP"
     gene            3369..4139
                     /locus_tag="VF531_05775"
     CDS             3369..4139
                     /locus_tag="VF531_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713510.1"
                     /translation="MYKVLIADDVAEICNGLSHYFPWHEFGFEVVGQAADGVQVLEFI
                     ASRPVDVLLCDIKMPVMDGIALAQELFNRKSPVKIVFISAYQEFEYAKKALAFGVVDY
                     LVKPTKYDDLVEVCYKIKTELDKASDDRSGNNPQTLSGLEMNGCNYQEQIVTTVKNYI
                     EDHFRDASLEGAAELVHMNSHYLSKFFKDKTGQNFSDYLIEVRMRKAAELLQNPAYLT
                     YQISDMVGYSSPKNFARTFKSFFGKTPREYRNYDNSVK"
     gene            4166..5959
                     /locus_tag="VF531_05780"
     CDS             4166..5959
                     /locus_tag="VF531_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7713511.1"
                     /translation="MQNIYSRFFIKNFLVFLIPLLIPLLILGAISTVAVRDFLKNEIN
                     KNNQNLLKLTQNNLELIFDEMDSLYLNFSTTPEISIKLKKILSNPYSGLAFDELDTIK
                     TIQIFLDTPANAHPYIHSIYVYFNNPNQKMITTTEGLTDLRAYYDTSWYDSYVQNGRN
                     LSLWTEPRIISQYAFEKKPTQVETIYRRLYTSGNPNAGVIVLNIKTEYIEHLLDNLET
                     LPDQGFMIVDENNRVIFRNRQAHSIGNDSLMKIINHPSNNFVYHSQQGDFTVSYLLSP
                     KYHWKYISVVPQQTLYAIPLKLQKFILLLLPGLFVLGLVLTYWLARNNYLRIQNIISI
                     IEAAKTNSTLPGLPAQINDEYGFITYNLLTTFIEQNYLKLQLSERKYKLQVMELLALQ
                     AQINPHFLFNTLDTIKWQIIGLAKKPNETSAMIEQLSEILKYSLEQPNKTVSLKEEIQ
                     NTRNYINILKILYRDKFDLIWDYDENVLDCQVIRLLFQPLIENSIYHGIKEKSGQSYL
                     KIKIQLQGGLLKIAVIDNGLGIRAEKLKEIRTHLDDGTEGKGHIGLVNTKKRLELTFG
                     EGTLFKVNSKLNLGTVITIRIPCPLNQEDIS"
     gene            6084..7376
                     /locus_tag="VF531_05785"
     CDS             6084..7376
                     /locus_tag="VF531_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7713512.1"
                     /translation="MKNLVKVIIPLCLIAVILFVSAFATYAAPANVTLRFSWWGTETR
                     HKATLAAIDLYMKGNPNVKIEAEYGGFDGYQQKLLVQLAGMEAPDVIQIDQGWLQDIM
                     AQGNLLEDLNKYKSALKIGNFDKKFLAQNCTVKGKLVGLPTGINSQTYVVNQNLLTKF
                     NIDPNTKWNWENLLEIGTRIHKQDSSCYLLVYDSNNIDLLRTYLTNKTGKQWINDDYT
                     LGFNKAQLVEAFTYYRKLLDSGTLEPIADSVIFENKIEQDSRWINGKIIMVSGWASTM
                     GVFKRPEFDLTVMCPVFGKNAKNTGISVRSSQVFSVNSRSAHKKEAAQFMNWLFTDPE
                     AIAALGMSRGVPPTEKAIQLLTEKNMVDKNTSTAVSLGVKNAGLPDNTFSTNNELLTI
                     GKDVFLKVGFGKLTPDQAAGELINSLKAKLAELKAQKK"
     gene            7461..8390
                     /locus_tag="VF531_05790"
     CDS             7461..8390
                     /locus_tag="VF531_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713513.1"
                     /translation="MSTNGNVIKDKPKKRSWVGLLYISPWLLGFLIFQLYPFFSSLYY
                     SFTNLSMFKAPDFTGIQNYSYMFTRDANFFQALKVTFVYVLFAVPAKIIVSLLVALIL
                     SQKIRGINFYRTVYYLPSILGGSVAVAFLWRILFMNDGIINKCLSFFRMSPLQWLGPD
                     LALFTLSLLTVWQFGSSMLLFLAGLKQIPPELYEAAKVDGASSLKIFFRITVPQLTPI
                     IFFNLVMQMINAFQEFTSVFIVTNGTGGPIGATYLYGLMVYRNGFEFFKMGYASAQSW
                     VLFVIIMFFTAFIFKSSPYWIYYEDGGDINGSK"
     gene            8377..9222
                     /locus_tag="VF531_05795"
     CDS             8377..9222
                     /locus_tag="VF531_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713514.1"
                     /translation="MAVNKRMVYTIVTHSYIVLFAIVMVYPLLWIIASSFKPSSEIFS
                     SPSLIPSQFVWDSYAKGWMGFGQQGFGLFVNNTFILVIPTVIFTLVSSALVAYGFARL
                     DFPLRKFFFALMLSTLMLPGTIVIIPRYLLFKNLNWLNTYLPFYIPALLAGNSFFIFM
                     LVQFLRGIPRELDESAFVDGCNSFLILYKILLPLCKPALFTVALMQFIWLWNDFFNPL
                     IYINSVSKYPLALGLRISIDVGAIVNWNQVLAMAVVAIIPPVILFVFTQKYFVEGIAT
                     SGLKG"
     gene            9743..10531
                     /locus_tag="VF531_05800"
     CDS             9743..10531
                     /locus_tag="VF531_05800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7713515.1"
                     /translation="MEKQLSTIDPQWYKKGWGLGIKNQSWVEDTENQVAFIIKTLGLT
                     GKERVLDLACGYGRHALSLARKGYSVVGVDITKDFIDDARKEAKSASLAIDFIQADIR
                     ELSYQNEFDAVLNLADGAIGYLENDAENLEIFDIIARALKPGGKHFMDICNAEHAEAF
                     FPKRHWEIGSTTLSLPGFDWDKETRRMLFSDWSIPFGEIARKPDSIEPFSSTRLYSRA
                     ELDDIFQARGMAIVDTFADYYGKEASYKELQLMVYSRKVIGNRK"
BASE COUNT         2864 a         2429 c         2401 g         2850 t
ORIGIN      
        1 catcaccgag cggtatccgt aaagtctgaa agttgatgtt caaaaccggc gaatcaaagt
       61 catcacgcaa tggttcatct tcgccgcggt attccgggag atttggtgcc tgaacctgca
      121 aaaagggatg attgcctccg gcttcgagcc gaagccaatt agcatcattt caaatcattt
      181 tttgaatcgc ggtctcccgt cccagaggat atctgccccg ggccggtatc ggccgactac
      241 ataaatgaac catataccat tcgccgtctt gagtctccac caaatcggca tgtcccgctt
      301 tttgcagcgg cagagtgggg tcatcccggg aagttaaaat tggatttgcc ggatcgactt
      361 cataaggtcc cgtaatcaac cgggatcggg ccattgtgac tacatggccg agccccgttc
      421 ctccctcggc ggtgatcagg taatagtaac ccttttgttt atacaggtgg ggcccttcgg
      481 tgcatcccag ttccgttccg ttaaagataa tggataccgg gccgattaag cgtttttcct
      541 ccggcgaata ctcttgcaaa gcgattccgc cgaagggatt cttgtctttg cgatgatcgg
      601 tctgggccca taccagccag tggcggccgt cgtcatcatg aaagagcgag ggatcaaagc
      661 cgcaactgtt tagataaaca ggctcggacc attcaccccg gatatcggcg gtggttacta
      721 gataattatg ggtatccttg gcgaatgtct tcgaggtttt gacatcggta tagactaaat
      781 aaaaaagacc ccgatcgaaa cttaggcatg gagcccagac gccgcacgag gccggattgc
      841 ctttcatgtc cagttgggaa agacggttga gcggatgggc ggttaatcgc caatgaacca
      901 aatcccggga gtgatgaatc tgaactccgg gaaaccactc aaaggttgag gtggcgatgt
      961 aataatcgcc attaacccgt acaatggagg gatccgggtt aaacccacgt aaaatgggat
     1021 tggtaataag cttcatgata tgaatcagtc accctttatg gcttttttag ccttatactt
     1081 ttagtgaggg tcggttgaac aacgccttac ggcatcggca gcaagccttc ccaggttacc
     1141 gtccagttcc cgtccttggg aaaccccggc gtatctccga actcacaacc atcccagccg
     1201 gccgccatca tagccaccgc agtcagcaac cctccgttgc ccggcaggta taacggtaaa
     1261 tccttccgtt cgccctgaca attgtggcca ttcggcagat atcgattttt aggcgaatcc
     1321 atcaacaggg cggccacggc aatatcaggc cggccgactc tggccgcagt catggcgatc
     1381 atcgggaaat cccaacccca catctcttcc agccgccatt cctgcaatac tttataaagt
     1441 gttttcatca tcgtgccgcg atccactttc atcccgggca acacccctaa tgctcccagc
     1501 atggatggat ggtccagatt aaagcggcta aatgtcgccg ggcaattctc atgagccaga
     1561 taaaatccat ccattacggg gagagcggag agtcgtgagg caatctcccg ccaggtctcg
     1621 ttttcgtcca accctaaccg tttgcgccac tggttggcgg tatttaagcc aaagcaccag
     1681 tattccagct caaatgtggg attgagggtg gtttccggtc tgtgattctc ctgggccgga
     1741 atgacgggtg gtccaagtac atagcgattg ttggtttcat catactggac atacgaagcc
     1801 ataaactcgg ccgtttccaa tacaatgtcc cggtatatct cgagagtccc gggagaggga
     1861 tgggcccggt aacacagttc ggcaagatag atcggatggg gttgctgcca tatcaataat
     1921 ggcccgatgg gggaggggct gtcgtatccg gcgggcccta ccattttcgg ccaacgggcg
     1981 cctcgatatc cttgagatgc cgctattcct ttagccagcg gtaaaatcgt ccgataccac
     2041 cagatactcc tttccagcag gcggcttctg ccccaaagcg gaaaatgggc cgcatgccac
     2101 caatgcatct ccagatgaaa tttcccatac cagctgttgc aggtaagacc ggtctcctgg
     2161 gggggcattg tcccggtgca ctggatggca gtcaggtatt gggacaaaat aatccggcgc
     2221 tccaattcgt ttgcctgcgg gtcacggctt ccggcgagtg taaccgcgcc gccttccaac
     2281 caaaactttt tccaatgctt ttcacaggct gcctggatat cggcaaagga gggtaaagct
     2341 tcaaatagcg gttttggcgt gaatgaaacc gaaaattcca gtttactgac accggtcgca
     2401 ggctccatca caaattcgtt gcggccgtcg cggtatagtc gcccggtatt tgaatggcga
     2461 atccccacga aatagcgatt gtgatcgaga atccgcatca aaccaaagtc cacaccatcc
     2521 atgggaatca tgacggtgtt atgtttttga tcactagacc aatcggcggc ggttttatcc
     2581 ggtgatccgt acggaaaagc aaggcggact ttcagacgcc cgcgcggtaa caattcggat
     2641 accacggaaa aggcaagaat cccctgggta gggtggcagc aggttttcac attaaccggg
     2701 acgcctccaa gcgtgaatct gctttccaga attccccgcc agagatcaag ttgctggtaa
     2761 atgttttgaa tatcatcagg ccaggccaga ttaccgtcgg acgttagcat ttccaaaccg
     2821 atccgtccca aatgcaaccg gtgggggttc aacctcagcc agtcatagat ctcttcctga
     2881 ccctcgttgc ttacagcata tccgactctg cggccggcta cgtcaaaata ttggagggat
     2941 agatcatgat gggtaaaata ccgaccatcg tccgccggag cggtatgcca accccactga
     3001 gattgagtac ataggggcat gttgtcacgg tatggctccg ggaaggtctg catgccggta
     3061 atatcggcag taaaggctaa ctcaccattg ccgatggaaa ggggagagcg ggggtcaagt
     3121 tttttcagga cggggttatg tcggcttaca agctgatagc ggtcaattgt cattgcatct
     3181 catctcgatt atgatgaaat tactgaattg aaacgcgctt gaattggatc attctcgatg
     3241 aagcggcgat atttctattt tgctaaatat atactataat ataataaata ggcatttaaa
     3301 tcaacttcgc cctgattgtt tttggtaaac accttaaaaa gatcatttca gtctacaagg
     3361 ataaggtcat gtataaagtg ctgatcgccg atgatgtagc cgagatatgc aatggcttga
     3421 gccattattt tccctggcat gagttcggct ttgaggtagt aggccaggcg gcagatgggg
     3481 tccaggtttt ggagtttatt gcctcgcgcc cggttgatgt tttgctttgt gatattaaaa
     3541 tgccggtcat ggatggcatc gcgctggccc aagagctttt taaccgcaaa tcgccggtta
     3601 aaatcgtttt tatcagtgct tatcaggaat ttgagtatgc caaaaaggcg ttggcattcg
     3661 gtgtggttga ttatctggtt aagccgacca aatacgacga cttggtagag gtttgttata
     3721 agatcaagac cgaactcgat aaagcatccg acgatagaag cggaaacaat ccacagacct
     3781 tatccggtct tgaaatgaat ggttgcaatt atcaagaaca gattgtcacc acggtgaaaa
     3841 attatatcga ggaccatttt cgggatgctt cactagaagg agccgccgag ttggtccaca
     3901 tgaattccca ttatcttagt aagttcttta aagataaaac gggccagaat ttttccgatt
     3961 atctgataga ggtgcgaatg agaaaggcgg cggaattatt gcaaaatccc gcctacctga
     4021 cctatcaaat cagtgatatg gttggatatt caagtcccaa aaattttgcg cggaccttta
     4081 aaagcttctt tgggaaaacg cccagggaat accgtaatta cgataattcc gtcaaatgaa
     4141 tgaaattatt gaaaaggttt aagccatgca aaatatatat tcccgcttct ttatcaagaa
     4201 ttttttagtt tttttaatcc ccctgctaat tccgctgtta attctaggcg ctatttcaac
     4261 cgttgccgtc cgggactttt taaaaaacga aatcaataaa aacaatcaga atctgctcaa
     4321 actgactcaa aacaatctcg aattaatctt cgacgagatg gactccttat acttaaattt
     4381 cagcaccact ccggagattt ccatcaaatt aaaaaagatt ttgagtaatc cttattccgg
     4441 cttagccttt gatgaactcg acaccatcaa aacgatccag atttttctcg atacacccgc
     4501 gaatgcccat ccctacattc actccatcta cgtttatttt aacaatccca accaaaaaat
     4561 gattaccact accgaaggac tgacggatct ccgtgcttat tacgatacat cctggtatga
     4621 ttcttatgtc caaaatggca ggaaccttag tttatggacg gaaccgcgaa tcatcagcca
     4681 atatgcgttt gaaaaaaaac ccacccaggt ggagaccatt tatcggcgtt tatatacctc
     4741 aggtaatcca aacgccggcg tcatcgtttt aaacatcaaa actgaatata tcgaacattt
     4801 gctggataac ttggagactc ttcccgatca gggttttatg atcgttgatg agaacaaccg
     4861 ggtcattttt cgaaaccgcc aagcccattc cattggcaac gatagcctca tgaagatcat
     4921 caaccacccc agcaataatt ttgtatatca ctcacagcaa ggggatttca ctgtctccta
     4981 ccttttatcc cccaaatatc attggaaata tatatcggtt gtaccgcaac aaaccctata
     5041 tgccattccg ctgaaactgc agaagtttat cctacttctt ttgccgggcc tatttgtatt
     5101 gggattggta ttaacctatt ggctcgcccg taacaattac ctgaggatcc aaaacatcat
     5161 ttcgatcatt gaagccgcca aaaccaactc cacgttgccg gggcttccgg cacagatcaa
     5221 tgatgaatat ggatttatca cttacaatct cctgaccact tttatcgaac agaattacct
     5281 gaaattacag ttatcggaga gaaaatacaa gttgcaggtc atggaacttt tagctttgca
     5341 ggcgcagatc aatccccact tcttatttaa cacccttgat acgatcaaat ggcaaatcat
     5401 cggtctcgct aaaaaaccca atgaaaccag cgcgatgatc gagcagctct ccgaaatcct
     5461 gaaatattcg ttggaacaac ccaataaaac cgtatccctg aaagaggaga tccaaaacac
     5521 ccgcaattac atcaatatct taaaaatatt atatcgtgac aaattcgatt taatctggga
     5581 ttatgatgaa aatgtcctgg attgtcaggt tatccgttta ctgtttcaac cattgattga
     5641 aaacagtatt tatcacggaa tcaaagaaaa aagcggtcaa agttatctga agattaaaat
     5701 tcaattgcaa ggcggtttac tgaaaattgc ggtcatcgat aacggcttgg gaataagggc
     5761 cgaaaaactt aaagaaatta ggacacacct ggatgatggc accgaaggga aggggcatat
     5821 cggtttggtc aacaccaaaa aacgcctgga acttaccttt ggagaaggaa ccctttttaa
     5881 agtcaacagt aaactgaact tggggacggt gattacgatt cgaattccct gtccgttaaa
     5941 tcaagaggat atttcttaaa aattgatacc ttcggtttgc gtcaaacgaa aaattgatac
     6001 tctttataac ccggacggcc ttaacttcct tgaaattctt tggtaacatt gacttaacaa
     6061 ataatccaag ggaggtattt tttttgaaaa accttgttaa ggtgatcatt ccgttatgtc
     6121 tcatcgcggt catccttttt gtttctgctt ttgccacata cgccgcaccc gctaacgtaa
     6181 cattacggtt ttcatggtgg gggacggaaa cccgccacaa agcgacattg gctgcaattg
     6241 atctctacat gaagggaaat cccaatgtaa agatcgaagc ggaatacggc gggtttgacg
     6301 gatatcaaca aaagcttctg gtgcagctgg ccggcatgga ggccccggat gtcattcaaa
     6361 tcgatcaagg ctggctccag gatattatgg ctcaaggcaa tttgctggag gatttgaata
     6421 aatacaaatc cgccttgaaa attggcaatt ttgataagaa attccttgcc caaaactgta
     6481 cggtcaaagg gaaactggtc gggctcccga ccgggatcaa ttcacaaact tacgttgtca
     6541 atcagaattt actgacgaaa ttcaatatcg atccgaatac caaatggaat tgggagaatc
     6601 tgctggaaat cggaacccgc attcataaac aggacagcag ctgctatctg ctggtttacg
     6661 actccaacaa cattgatctg ctccggacct atttgaccaa taaaaccggc aagcaatgga
     6721 ttaatgacga ctatacctta gggtttaaca aagcccaact ggttgaggcc tttacctatt
     6781 accggaaatt gttggacagc gggaccctgg aacccatcgc cgatagcgtg atatttgaaa
     6841 ataagattga acaggacagc cgctggatta acggaaaaat tatcatggtc tccggctggg
     6901 cgtcgaccat gggagttttc aaacgcccgg aatttgacct gacagtgatg tgcccggttt
     6961 tcggcaaaaa tgccaaaaat accggtataa gtgttcgttc ttctcaggtg ttttccgtca
     7021 acagcagatc ggctcataaa aaagaagcag cccagttcat gaactggtta tttaccgatc
     7081 ctgaagccat tgcggcgctg gggatgtccc gcggcgtgcc gcctacggaa aaggccatcc
     7141 aattgttgac cgaaaagaat atggtggata agaatacctc caccgctgta agccttggcg
     7201 ttaaaaatgc cggtctgccc gataacacct ttagcaccaa taacgagttg ttaaccatcg
     7261 gcaaggatgt cttcctcaag gtcggattcg gcaaactcac tccggatcag gcggccggtg
     7321 aattgatcaa cagtctcaaa gcgaaactgg ctgaattaaa ggctcagaag aaataattat
     7381 catcaataac tttaaggaat accggtcagc cgaccggtat tccttacaat tggaagtcca
     7441 cgcgaatgga gccttgaaag atgagtacaa acggtaatgt cataaaagat aagccaaaaa
     7501 aacgcagttg ggtgggttta ctttacatct ccccgtggtt gcttggattt cttatttttc
     7561 aactgtatcc ctttttttca tcattgtatt attcctttac caatttaagc atgtttaagg
     7621 ccccggactt taccgggatt caaaactata gctatatgtt tacccgggac gccaatttct
     7681 ttcaggcatt aaaggtgaca tttgtctatg ttctatttgc cgtccccgcg aaaattatcg
     7741 tttctttact tgtcgccctc attttaagtc aaaaaatcag aggaatcaat ttttaccgca
     7801 ccgtctacta cctgccttca attttgggag gaagcgtcgc cgtggcattt ctgtggcgga
     7861 tcctctttat gaatgacggg atcatcaata aatgcctttc gttttttcgg atgagtcccc
     7921 tccaatggct gggaccggat ctggcattat tcaccttgag tctgctgaca gtatggcaat
     7981 ttggctcttc catgctgctt tttttggcgg gcttaaaaca gatcccgccg gaattgtacg
     8041 aagccgcaaa agtggatggc gcctcaagcc ttaagatatt ttttcggatt accgttcccc
     8101 agttgacccc gatcatcttt tttaacctgg tcatgcaaat gatcaatgcc tttcaggaat
     8161 tcacctcggt atttatcgtc accaacggta ccggcggccc catcggagcc acttacttgt
     8221 atggattgat ggtttaccgc aacggtttcg aattctttaa aatgggctac gcttcggccc
     8281 aatcctgggt gttgtttgtg atcatcatgt tcttcacggc gtttatcttt aaatcttcac
     8341 cgtactggat ttattatgaa gatgggggtg atataaatgg cagtaaataa acgtatggtt
     8401 tataccattg taactcatag ctatatcgta ttgttcgcaa ttgtaatggt atatccactg
     8461 ctgtggatca tcgcctcttc ttttaaaccc agttcggaga ttttttcttc tcccagtctg
     8521 atcccgtctc aattcgtctg ggattcctat gccaagggct ggatgggctt cggccaacag
     8581 ggcttcggtt tatttgtgaa caataccttt attttggtca ttccgacagt aatttttacg
     8641 ttggtatcca gcgcactggt ggcttatgga tttgcccggc tggattttcc cttgcgcaag
     8701 ttcttttttg cattgatgct ctccacatta atgctgcccg gaaccattgt cattatcccg
     8761 cggtaccttt tgttcaagaa cttgaactgg ctcaatacct atttaccgtt ttacatcccc
     8821 gcgctcttag ctggaaattc cttttttatc tttatgctgg tccaattttt acggggcatc
     8881 ccgcgggaac ttgatgaatc agcctttgtg gacggctgca attcgtttct catattgtat
     8941 aaaatcctgc ttcccttatg taaaccggcc ttgtttaccg tggcgttgat gcaatttatc
     9001 tggctgtgga atgatttctt caatcccttg atttatatca acagcgtcag taaatacccc
     9061 ctggcgctcg gactccgcat ctccattgat gtcggcgcca tcgtaaactg gaaccaagtc
     9121 ttggctatgg cggtggtggc gatcattccg cccgtcattc tatttgtctt tactcaaaaa
     9181 tattttgtgg agggaattgc cacctcggga ctgaaaggat aatgatgatt acattctcgt
     9241 gaggatatac cggagtttta acttgactac agtaagatgc ggggctatgg aatacggccc
     9301 cgttttttat attatatcgt cagatcagga aatgattgaa ggtaagctag gaaagttgta
     9361 gaaagagtaa atatattcta ataactcaaa tcacattggc atagcgatat gtaatatttt
     9421 gaggctggga gggaccgcct catcccgttt ccattaagtt taccgggtgc catttggaaa
     9481 agtttctatc tgtatgcctg ttttattcac aacggaacac acaaaaaaca taaaaatggg
     9541 tatggcttta tttgcggcta ttgaagtaat ttatccatca tacgcaataa accatttgta
     9601 ttttgggata aaaaaaaccc gagacaacga tctcgggtca cactataaca ataggataac
     9661 tcaaaataaa taaaaagtct atacttttcc gaaaacctcc gctatattca ataccagagt
     9721 aaacgaaacg gaggttttaa gaatggaaaa gcaattgagt acgatagatc cgcagtggta
     9781 taaaaaaggc tggggactgg ggattaaaaa ccaatcctgg gttgaagaca cggaaaatca
     9841 ggtggctttc atcatcaaga cgctggggct gacgggaaaa gagcgcgtcc tcgatctggc
     9901 ctgcggctac ggtagacacg ctctttcttt ggcacgaaaa gggtattccg tggtgggagt
     9961 cgatattact aaagatttta ttgatgatgc gaggaaagag gctaaatcag cctctctggc
    10021 aatagacttt attcaggcgg atattcgaga gttgtcatat caaaatgagt ttgatgcagt
    10081 gttaaatctg gccgatggag ccatcgggta tttggaaaat gatgcggaga atttagagat
    10141 ctttgatata attgcccgcg cccttaagcc cggtggcaag cactttatgg acatctgtaa
    10201 cgccgaacat gccgaagcct ttttccccaa acgccattgg gagattggtt ccaccacctt
    10261 atcgttaccg ggatttgact gggacaaaga aacccgtaga atgctgttta gcgattggag
    10321 tattccattc ggggaaatcg cccggaagcc ggattcaatc gaaccttttt cctcaacacg
    10381 cttatattcc agagctgagt tagatgacat ttttcaagcg cggggaatgg cgatcgtgga
    10441 tacatttgcg gactattacg gtaaggaagc gtcatataag gaattgcagt tgatggtgta
    10501 ttcgaggaag gtaataggca ataggaaata ggcaacgggc aaca
//
LOCUS       DASVMB010000192        11248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_318124,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000192 DASVMB010000000
VERSION     DASVMB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318124"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF531_05805"
     CDS             <1..349
                     /locus_tag="VF531_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7713516.1"
                     /translation="PIFFDFDSAMIRADQQGALEQDVQILQTVKGYILIGGHADPFGE
                     REYNIGLSRRRAQAVADYLIAHGIDPARIVIFAYGKDHPEAKRKAEGWPSDRWADIVI
                     TVEEPRMEMGIRK"
     gene            501..1466
                     /locus_tag="VF531_05810"
     CDS             501..1466
                     /locus_tag="VF531_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7713517.1"
                     /translation="MMKKYLIFLGIMILAVSFSGCMDPASRRVSKTSTPAAVTYVYNT
                     QWGSLGSGNGQFNSPRGMAVDSSGNVYVADTFNNRIQKFSSAGTYITQWGEDGNGNSL
                     LNQPNGIAVDFSGNVYVADTQNCSIEKFNSNGIYITHWGASGGGDGQFGYPEGVAVDS
                     SGNVYVSDYQYPRIQKFSSAGTYITKWGSNGTDNGQFYSPIGIAVDSSGNVYVADTNN
                     PRIQKFSSDGIYITQWGSSGSGNGQFILPNGIAVDSSGNVYVADAYNHRIQKFSSDGI
                     YITQWGSSGSGDGQFNYPRGIAVDSSGNVYVADTGNNRIQKFSPQ"
     gene            1609..2574
                     /locus_tag="VF531_05815"
     CDS             1609..2574
                     /locus_tag="VF531_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX7713518.1"
                     /translation="MKKSCFYGLMAVLLLAVILSGCGGGGSNPPNPPTYASGSQWASA
                     TSAAAFTARLNHTSVVYDNKMWVIGGDDESESSCLNDVWYSGDGVKWTQVIVNVPFSA
                     RIGHTSVVYDNNMWVIGGYDGSNYLNDVWSSSDGTTWTQVETGRIFSARTGHTGVVYK
                     NKIWVIGGYDGSHLNDVWSSSDGKTWSQETTGAAFSGREGHTCVVYDNKLWVIGGGNS
                     SGLLNDVWSSSDGKTWSQETGNAFSPRYAHTSVVYNNKIWVIGGYDNSAALNDVWYSG
                     DGANWTQATPSAAFSARHYHTSVVYNNTIWVIGGSGYQNDVWYAQ"
     gene            2877..3668
                     /locus_tag="VF531_05820"
     CDS             2877..3668
                     /locus_tag="VF531_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713519.1"
                     /translation="MRKWQHSGILFLMILLIILSGCTKKETIIKETDPTPTKRVARIL
                     VVNDELSPVLNFVGQGLAKLQTTRSFYYKTATSIDNFNDLAATGYWDLFVVNNTSTSH
                     LQLLNNIKNHIDGGGGVVFTSFQMLGSTHRVWASLGASFGILSTAVADNYRWDPGNAI
                     FTTPYSVPDMTGMTNFKGFGNCIIRGTQLAGCSAVAGSTASSQSDQATIFVANEGRTV
                     LNSFMIDCMADAVGAPRDNDSDGVADAVELWANEINYVLDACWCM"
     gene            complement(3762..4460)
                     /locus_tag="VF531_05825"
     CDS             complement(3762..4460)
                     /locus_tag="VF531_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713520.1"
                     /translation="MDEKRILLVDDDPAVLKLVDAALVKNGYLTVQTTSGELALEVLE
                     REEVAAVILDVILPGIDGLETLKRIRGNGRYGQVPVLMLTCRDEEIDSVVGLELGADD
                     YLAKPVRYHELIARLKNIFRRIQPPNPAHSTKINRNGLEIDCNARTVKTSAGVDLKLT
                     FLEFEIFSLLADYPGKVFSRDDILDKIWTDETLYETRTVDSHIRRIRGKLREAGVSPE
                     MIETVRYIGYKFTE"
     gene            4715..4861
                     /locus_tag="VF531_05830"
     CDS             4715..4861
                     /locus_tag="VF531_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HEX7713521.1"
                     /translation="MAFDGGYLQSLALKSDGTVWAWGMNTYGQLGDGTTTDSRTIPVQ
                     VRGL"
     gene            4912..5088
                     /locus_tag="VF531_05835"
     CDS             4912..5088
                     /locus_tag="VF531_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713522.1"
                     /translation="MSGLYQWMGFNYLVHAKAVLLAGNYLKLEVLCEDMQQVFAQFSN
                     LYGYLHVHLMNAAA"
     gene            5104..5523
                     /locus_tag="VF531_05840"
     CDS             5104..5523
                     /locus_tag="VF531_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7713523.1"
                     /translation="MEKAKKTLGRALEIGRTDRIILPFAEYGFYILDILKALKNESGN
                     DAYLDRLVKETARYSENLKLLNGAITTIGELTEREQEILQFVASGQTNQEIAAGMHLA
                     EITVKKNISAINRKLEVGGTGGGREKGGGVEVDVSVY"
     gene            5706..6548
                     /locus_tag="VF531_05845"
     CDS             5706..6548
                     /locus_tag="VF531_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713524.1"
                     /translation="MKKISPFSLTVILILAMVLTGCGSGSGGGSSPNADEQAINTVFN
                     SIGIDASSFFSSDNIASSIAPVHSDSSSDPYFTWNSVIFTANGYTQRLTNIIFDSATA
                     AHATANFSVSAAYTITGYAIDSYHASWSNSGTLTLSGSGTIKFVKTDGAWSVDAIPDA
                     IYLSSSTSSPTIENLSIPARTSTGQTETFSGTMTSKDTQYFAAYGHMTGFKLTGFTRI
                     GHYDRSSGSYNISEDITFPSFTGNIYGVISIIELASDGYYISNTRYLNACAYIGMIKE
                     TTGS"
     gene            7358..8347
                     /locus_tag="VF531_05850"
     CDS             7358..8347
                     /locus_tag="VF531_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7713525.1"
                     /translation="MKKYGNVVLFLILILVPVLAGCSGGNGGGGNSGGGGYTGVAFTM
                     VPVPTVTNFPIGTDDNTDNPGSVTNPYYIAQTEVTYELWKAVYDWATDPARANRYYFQ
                     NAGVEGSMGTVGADPTTANNQHPVTNVSWRDAMVWCNALTEYYAAKGGTSLACVYTYN
                     GATVRDSRDSNVTACDSVAANIAANGFRLPTGNEWELAARYISATKFYPGNYASGADA
                     ACNATATADYDGDNYKHNTSDVAVYDNSSTAAVKSKSPNKLGLYDMSGNVWEWCFDLH
                     GDNDGIPLREVRGESWHYFDIMNEYQYLQLGFKDICNSDWFDSEIGFRPVRTQ"
     gene            9027..10106
                     /locus_tag="VF531_05855"
     CDS             9027..10106
                     /locus_tag="VF531_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7713526.1"
                     /translation="MKKYNHFILILLLLALTLSGCGGGNGGGGNPGGGSPGSGDPGGG
                     NPGGGTTYTGVAFKMVSVPMVSSFPAGVDDKSSGSVTSAYSIAETEVTYELWSAVYAW
                     ATDRTATDSDTVPGHGQYTFGHAGLIGNDGSGGAKEPVTTISWHDAMVWCNALTEYYN
                     AKGGTGYACVYCTDITYNTPIRTVVTTTGTYTIDSGEGSQDNPCVNPIARGFRLPTSN
                     EWELAARYIGDKNGDGDIRDSGEYYPGAYASGADADYNDTTATTDIDGDGDQQIISDV
                     AECAGRGTAEVKSKDPNKLGIYDMTGNVYEWCLDWYDSATRMLRGGCCSVSTNSVPYL
                     RLGSTSGFGPAGTNFEVGFRFARTE"
     gene            complement(10401..11174)
                     /locus_tag="VF531_05860"
                     /pseudo
     CDS             complement(10401..11174)
                     /locus_tag="VF531_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309359.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
BASE COUNT         2989 a         2490 c         2833 g         2936 t
ORIGIN      
        1 cccgatcttt ttcgacttcg acagcgcaat gatccgtgcc gatcaacaag gggcactgga
       61 acaggatgtt caaatcttac aaacagttaa aggctatatc ctgatcggcg gccatgccga
      121 cccgtttgga gagcgggagt acaatatcgg gctgtcccgc agacgggccc aggcggtggc
      181 cgattatctg atcgctcatg gcatcgaccc ggcgcggata gtgatctttg cttacggcaa
      241 agaccatcct gaggccaaaa gaaaagcgga aggctggcca agcgaccgct gggcggatat
      301 cgtgattacg gtggaagagc ctaggatgga aatggggatc aggaaatgaa atgtgaagaa
      361 tgaagtcgtg cacacgcgcg tagaagtgtg aaatatggag gttaggatat cgttgccgac
      421 tgtcggctga ttttagagaa acaggcggtc aaattttacc ctttacactt taccctttta
      481 actttataca cgggggcttt atgatgaaaa aatatctgat ttttttaggg attatgattt
      541 tggcggtcag ttttagcggc tgtatggatc cggcgtcccg gagggttagt aaaacaagca
      601 cgccagcggc tgttacctac gtctacaaca cccagtgggg ttccctcggc agcggtaacg
      661 gacagtttaa ttctccgagg ggaatggcgg tagattcctc cggcaatgtt tatgtggcgg
      721 atacttttaa taaccgtatt cagaaattca gctccgcagg aacatatatc acccagtggg
      781 gtgaagacgg aaatggtaac agcctgctta accaaccaaa tggaatcgcg gtagatttct
      841 ccgggaatgt ttatgtggcg gatactcaaa attgctctat tgaaaaattt aattccaatg
      901 gtatatatat cacccattgg ggcgcctccg gcggcggtga cggacagttt ggttatccag
      961 agggagtcgc ggtagattcc tccggcaatg tttatgtgtc tgattatcaa tatccacgga
     1021 ttcagaaatt tagctccgct ggtacatata ttaccaagtg gggttccaac ggcacggata
     1081 acggacagtt ttattctcca attggaatcg cagtagattc ctccggcaat gtttatgtgg
     1141 cggacacgaa taatccacgg attcagaaat ttagctccga tggtatatat atcactcagt
     1201 ggggttcctc cggcagcggt aacggacagt ttattcttcc aaatggaatc gcggtagatt
     1261 cctccggcaa tgtttatgta gcggatgctt ataatcaccg tattcaaaaa tttagctccg
     1321 atggtatata tatcactcag tggggttcct ccggcagcgg tgacggacag tttaattatc
     1381 cacgaggaat cgcggtagat tcctccggca atgtttatgt ggctgatacc ggaaataacc
     1441 gcattcagaa atttagtccg cagtgaaaag aatgggcgcc tttatatttc aaaatgtcac
     1501 acggccgtca cctgaaaacg attgattgtc acaatcatga tctataattc taccagggca
     1561 ttagtttcaa tccccttttt acctgaaatg tccaaggagg ttttcccaat gaaaaaatct
     1621 tgtttttacg gtttaatggc cgtgctgctt ttggccgtca tcctctccgg ttgtggaggc
     1681 ggaggcagca atccacctaa tccacccaca tatgcctccg gttcgcaatg ggccagcgcc
     1741 acgtccgcgg cggcgtttac agcgagactt aatcatacca gcgtcgtgta tgataacaag
     1801 atgtgggtca tcggcggtga tgatgagtct gagtcaagtt gtctgaatga tgtttggtat
     1861 tccggcgacg gggtaaaatg gacccaagtc attgtcaacg tgcctttttc ggcgaggatc
     1921 ggtcatacca gcgttgttta tgacaacaac atgtgggtca ttggtggtta tgacggttca
     1981 aactacttga atgatgtttg gtcttccagt gatggaacaa cttggactca agttgaaacc
     2041 ggaaggattt tttcggcgag aacggggcat accggcgttg tgtataaaaa caagatttgg
     2101 gtcatcggcg gctacgacgg gagccacctt aatgatgttt ggtcctccag cgacgggaaa
     2161 acgtggtccc aggagaccac cggcgccgct ttttcaggga gagagggcca tacatgtgtt
     2221 gtttatgata acaagctgtg ggttatcggt ggtggtaatt ctagcggcct cctgaatgac
     2281 gtttggtctt ccagcgacgg gaaaacgtgg tcccaggaga ccggcaacgc tttttcaccg
     2341 aggtacgccc ataccagcgt tgtttataat aacaagatct gggttatcgg cggttatgat
     2401 aattcggctg ccctgaatga cgtgtggtac tccggcgacg gggccaactg gacccaggcg
     2461 acccccagcg ccgctttttc ggcaagacat tatcatacaa gcgttgttta taataacaca
     2521 atttgggtca tcggtggttc gggctaccag aatgatgttt ggtatgcgca gtaattttgc
     2581 cgttgcaata acttcagttc atcaccggcg ccggttttaa cggggcgtta caatcaatct
     2641 ccggcccgtt tcggctaatc ttgcggaaac gattccttcg ggaatgttta tgtggcggat
     2701 accggaaata acccattcaa aaattcagtc cacagtgaaa tgaatgggag gctttatatt
     2761 tgggttgaac gcttccccac gtcttcgtgt ccttcgtggt agataataat ttataaaaga
     2821 ttgtccattc ggacgatgcg caatgtatca tcccaaaaag ggggcagaaa ttgattgtgc
     2881 gaaaatggca acattcggga attttgttct taatgatcct gcttataatt ctttccggtt
     2941 gcactaaaaa agaaacaatt ataaaagaaa cagatccaac tccgacgaaa cgggtggccc
     3001 ggattttagt ggtcaatgat gagttatcac cggtgctgaa ttttgtaggg caaggccttg
     3061 ctaaattaca aaccaccaga tctttttatt acaagactgc aacaagtatt gataatttta
     3121 atgatttggc cgccactggg tattgggacc tgttcgtagt caataatacc agtacctctc
     3181 atctacaact cttgaacaac attaaaaatc acattgatgg gggcggcggg gtggtgttta
     3241 ccagcttcca aatgcttggt tccacgcacc gggtctgggc tagcttggga gcgagttttg
     3301 ggatactaag caccgctgta gccgataatt accggtggga tcccggtaat gcaatcttta
     3361 ccactccgta ttcggtgccg gatatgaccg gaatgaccaa ttttaaaggc tttggtaatt
     3421 gtataatacg gggaacgcaa ttagccggct gttcggctgt ggctgggagt acggcttctt
     3481 cacagagcga tcaggcaacg attttcgtag caaatgaagg ccggacggtg cttaattcct
     3541 tcatgattga ttgtatggct gatgccgttg gtgcgcctcg ggataatgac agtgacggag
     3601 tcgccgacgc cgtcgaactt tgggcgaatg aaatcaacta tgtgttggat gcgtgttggt
     3661 gtatgtaaaa atctacgcga taaataaaat taatcggatt aaagggatgg ctcaagagtt
     3721 acaataagca aaatagttca cagggggtga gttgtagtta gtcattccgt aaatttatac
     3781 ccgatataac ggacagtctc aatcatttcc ggtgagaccc cggcttccct tagtttgccg
     3841 cggatgcggc ggatgtggct gtccacggtg cgggtctcat ataaggtttc atccgtccag
     3901 atcttatcca aaatatcgtc tctggagaag accttgccgg ggtaatccgc cagcaacgaa
     3961 aaaatttcaa actccaaaaa ggttaacttt agatcaacac cggcgctggt tttaacggtg
     4021 cgggcgttac aatcgatctc cagcccgttg cggttaatct tggtggaatg ggccggattt
     4081 ggcggctgaa tgcggcggaa aatgtttttc aaccgcgcga tcagttcgtg ataacgcacc
     4141 ggtttggcga gataatcgtc ggcgcccagt tccaacccga ccacgctgtc gatttcctca
     4201 tcgcggcaag taagcatcag gacgggaacc tggccgtaac gcccgttgcc gcgaatccgt
     4261 tttaaggttt ccaacccgtc gatccccggc agtatgacgt cgaggatgac cgcggccacc
     4321 tcttcccgct ccaacacttc caacgccagt tccccggagg tagtctgtac ggtcaggtag
     4381 ccgtttttga ccagggcggc gtccactaac ttcaagacag ccggatcgtc atccaccagc
     4441 aaaattcgtt tctcgtccat aaggttcatc ctttaacttt atccttaatt gacttaattt
     4501 taacatttta gctggtaagc ggcaataaaa aaaggccgtt aaccgtctca gggatttttc
     4561 aatggcaagt cactatttag gtgtgtaaaa agggatgaat taatttcttt gcagttggta
     4621 tctcaaaagg tacctttttt cacctcaaaa taaaaaggga gggttaggaa acgaagggcc
     4681 agcggataaa atagttttaa aaagcatgta agtcattgct ttcgacggag gatatctcca
     4741 aagcctggct ttaaaaagtg acgggacggt ctgggcctgg ggaatgaata catacggcca
     4801 gttgggggac gggaccacca ccgacagtag gactatcccg gtacaagtca gggggttata
     4861 aactcaccct ttttgtttct ctctcttttg cggaggagag ggaaaaaggt aatgtcaggg
     4921 ttataccagt ggatggggtt taattacctg gtccatgcca aagcggtttt attggcgggg
     4981 aactatctaa aattggaagt gctctgtgaa gatatgcagc aggtgtttgc tcaatttagt
     5041 aatctctatg gttatctgca cgtccatctc atgaacgccg ccgcctaata caagttctac
     5101 gggatggaaa aagccaagaa aacgctggga cgggcgctgg agatcggtag gaccgaccgg
     5161 attattttac catttgccga gtacggcttt tatatccttg atattttgaa agccctgaaa
     5221 aatgaatccg gaaacgatgc ctatttggac aggctggtaa aggaaacggc ccgctactct
     5281 gaaaatttga agcttcttaa cggggcgata accactatag gagagttgac ggaacgggaa
     5341 caggaaatat tgcagttcgt ggcctcggga caaaccaacc aggaaattgc tgccgggatg
     5401 catctcgcgg agattacggt taaaaaaaat atcagcgcca ttaaccggaa attggaggtc
     5461 ggtggaacgg gcggcggccg tgaaaaaggc ggtggagttg aagttgacgt gagtgtttat
     5521 tgataaaaaa tgtatacgat cgtataatat aaaaatttcg ggaactctgc taagatctat
     5581 tcttaggaga gttttttatt tgattcatcc ttttttatca agtaaagcaa aatgtaagag
     5641 aacgcgaaat tccaggttct tggtaacttg gttaatagtt acaataaaag gaggcctttt
     5701 aaaagatgaa aaaaatctcc ccctttagtt taacagtcat actgattttg gcaatggttc
     5761 tgaccggctg cggaagtggc agcggcggcg gtagttcacc aaacgccgac gaacaggcta
     5821 ttaatacggt atttaattca attggcattg acgccagttc atttttcagc tcagataaca
     5881 tagcctctag catcgcccct gttcactctg attctagtag tgacccttat tttacgtgga
     5941 attcggtaat atttaccgct aatggctata ctcaacgcct gacgaatatc atttttgatt
     6001 cggcgaccgc agcccatgcc accgccaact tttcggtatc ggccgcttat acgataaccg
     6061 gctatgctat tgactcttat catgcaagtt ggagcaatag cggcacccta actttgtcgg
     6121 gatccggaac gattaaattc gtaaagacgg atggcgcgtg gtccgtcgat gcaatacccg
     6181 acgcgatata tctctccagt tcaacatctt cccctacgat tgagaatctt tccattcccg
     6241 cgaggacttc aacgggacag accgaaacgt ttagcggcac gatgacttcg aaggatactc
     6301 aatattttgc ggcatacggt catatgactg gatttaagct caccggtttt acacgaattg
     6361 gccactatga cagatcatct ggttcctata atattagcga agatattacc ttccctagtt
     6421 tcacggggaa tatctatggg gtaataagca ttatcgaatt ggccagcgat gggtattata
     6481 tctccaatac tcgctatcta aacgcctgtg cttatatagg catgattaaa gaaactactg
     6541 ggtcataata accgttaaaa ggtttattaa gcggtatttg tttaagatta gtcgggaata
     6601 aggccgatac agtaaagcgg ttgttgcgtg atcgatgcgc agcaaccgtt ttttattatc
     6661 aggcagttta gtaaaattaa tggctatgtt cttaaaatgg ttgatctact atctcctttt
     6721 agctttaatt tgggtcaacc acccctatgg ggctgtctca aaagcaaaca atggataatc
     6781 gtccttatcc ccaccaggcc ctttaggagc ctttctacgc acggaaaaat catctcaagc
     6841 cccatgaatg gggctgggta atacatccag ttcttcccag cccgttttaa cgggctttag
     6901 aatggttttt aacttagccc ggggttttaa ccccgggtgg acggaatgat aaatcggact
     6961 tttgagacag ccccagtcgt aatgagtatg cggggacacc ttgtgtccct tggacacgac
     7021 accccggcag cagcgcgtcc tctgggacgc gacccatgct gcacttgcgt aaagattaat
     7081 cctattctct gcgcctccac gcctctgcgg gagaatgaaa aaatgggcct ataggatcaa
     7141 ccattattga aaacatcgtc taaaattaat tttttattgt aaaatggcaa taaaagtaaa
     7201 aaacctgtac ttcggtacag gttacaaaaa tgagacaaaa ttataaaatt gaatggaacg
     7261 gagtaataat tataaaatca aaagagaaaa cctcgatcga gcggatttct gtttgatttg
     7321 taataagaca aattgcaacg gaaggaactt aattcaaatg aaaaagtatg gcaatgttgt
     7381 tctgttttta atcctaattc tggtcccggt cctcgccggc tgcagtgggg gaaatggcgg
     7441 cggaggcaat agcggaggcg gcgggtacac cggcgttgct ttcacgatgg ttcccgtccc
     7501 gacggtaacc aacttcccaa tcgggacgga cgataatact gataatcccg gttcagtaac
     7561 aaacccttac tacatcgccc aaaccgaagt gacgtatgag ttatggaaag cggtctatga
     7621 ctgggcgacc gatcctgcta gagcaaatcg atattacttt caaaacgccg gagtggaagg
     7681 gagcatggga acagtcggag ctgaccccac caccgccaac aaccagcatc cggtgactaa
     7741 cgttagttgg agggacgcta tggtctggtg caacgccctg acggagtact atgccgccaa
     7801 aggcgggacg agccttgcct gcgtatatac atacaacggc gctaccgtcc gggattcaag
     7861 ggattctaac gtgacagcat gcgatagtgt cgccgcgaat atagccgcca atggttttcg
     7921 cctgccaacc ggcaatgaat gggaactggc ggcgcggtat atcagcgcga ctaagtttta
     7981 tcccggtaat tacgccagcg gggcggacgc cgcttgtaat gctacggcaa ctgccgatta
     8041 tgacggagat aactataaac ataacaccag cgatgttgcg gtatatgaca acagttcaac
     8101 tgctgcggta aagagtaaat cacccaataa attaggtctt tacgatatga gcgggaacgt
     8161 ttgggaatgg tgctttgatt tacatggcga taacgacggc attcccctcc gggaggtacg
     8221 cggcgaaagt tggcattatt ttgacatcat gaacgaatac caatacctgc agcttggttt
     8281 caaggacatc tgcaattcgg attggttcga ctccgagatc ggcttccgcc ccgtgaggac
     8341 tcagtaattt tgaccatgga ttttcaggat taacaggatt aacaggatta accggattag
     8401 ggagggcgtg ttaagtcgat aaacgtcggc agttgcacag gaacagagag gctactcaga
     8461 aaatactttg gtgttagatc cctcttcttt cgtctcctcc ccgtccggag aggaaagaac
     8521 caccatgctt caaaggcatt aagacctcta ccagagcgtt tttagaaaaa cgtaggccag
     8581 cgttacctgt ccccggacgg ggaaaggcaa ggtaaggggt ctagcttatt ttcatccttt
     8641 cacgtgcccg tggctagcct gctagccagg gatgggcgac tatcgaaaat acttttagcc
     8701 taaatatttt taaagtaagg cggttgttgc ataatcaata cgcagcagtc gtttttttat
     8761 gaaaggctat aagcccggtc tttgattatc agacagttta gcaaaaaaca tttttattac
     8821 aagaccgatt ttaattataa aaaaggaata aaaagtaaaa aacctgtact tcggtacagg
     8881 ttacaaaaat aaggcggtat tataaaattg aatggaaaca aacaataatt atgaaatcaa
     8941 aagtgaaacc cccaatatca ccaatggacg gaacggtttt ctgtttgatt ggtaataaga
     9001 aaaaattcaa cgaaaggacg aatcgaatga aaaaatataa ccattttatc ctgatcttat
     9061 tactcctggc cctgaccctc tcaggctgcg gcgggggaaa tggcggcgga ggaaatccgg
     9121 gtggcggaag tcctggtagt ggagatccgg gaggaggcaa tcccggcggt gggacaacct
     9181 acaccggtgt tgccttcaag atggtctccg tcccgatggt aagcagcttc cccgctggcg
     9241 tagacgacaa aagttccggt tcggtaactt cagcctattc tattgccgag acggaggtaa
     9301 cttatgagct atggagcgcg gtctacgcat gggcgacgga cagaacggca accgatagtg
     9361 atacggttcc cggccatggc caatacactt tcggccatgc cggtcttata ggaaacgatg
     9421 gttcgggcgg cgccaaagag ccggtaacca ctatcagttg gcatgacgcc atggtttggt
     9481 gcaacgcctt gacggaatat tataacgcca aaggcggaac gggctacgcc tgtgtatatt
     9541 gtactgatat tacctataat accccgatca ggactgttgt tacaacaact ggtacttata
     9601 ctattgattc cggtgagggc tcccaggaca atccttgtgt taacccaatc gctagaggct
     9661 tccgcctacc gacaagcaat gaatgggaac ttgcggcgcg gtatatcggc gataaaaacg
     9721 gcgacggcga tatccgcgac agcggcgagt attatcccgg cgcatacgcc agcggggctg
     9781 atgccgatta taacgataca acggcaacta ccgatataga cggtgacggc gatcaacaga
     9841 taatcagtga tgtcgcggaa tgtgcaggta gaggcaccgc agaggtaaag agtaaagatc
     9901 ccaacaaact cgggatctat gatatgaccg gaaatgtcta tgagtggtgt ttagattggt
     9961 atgacagcgc aacgcggatg ctgcgcggcg gatgctgtag cgttagtact aatagcgttc
    10021 cttacctgag gcttggcagt acgtccggtt ttggccctgc cggaaccaat tttgaagttg
    10081 gtttccgttt cgctaggacg gaatgaagtt tgaatggcac tagccggagt gggggactat
    10141 ccggaaaatg cttcgatgct tttattgaac cacgaaatat acgaaaatca cgaaaaagga
    10201 aggattaaaa gcggtccctt ttcgtgtgtt ttgtgccttt cgtggttaag cgctctccag
    10261 ttattatatt tatattttca ttctttcacg tgccccggca aggcggggtg aacgaccact
    10321 tagaaaatag cttgtccatc cacgtcaatt catttggcgc tacaggcccg cctctttgta
    10381 tcttgcttgc aattggataa tcaagaagaa acaggccgat tctccatcaa ccaatactgg
    10441 ctgccgtcgg gtttccggtc catgaagcca tactcgatca gataccttcg tagtgtcaca
    10501 taatccggat aagcgttttt caagatggcg ttgacctccg tttcgctgta taaacggccg
    10561 ggttcaaagc gtttggcgat ctgcctcagg accaccagct tattcttctc tctgatctcg
    10621 aaggttttga gcggacctcc cggacctttc gggaagtaac gcttgaggat ttgatcgttc
    10681 tcatccgggg tgattttata ccggtcatcc accattcggg ccgtgtcgtg gggaggaata
    10741 aaccggggtg acgtttgatc cctcgtgttc aataactaca tcagggtcaa aaagactttg
    10801 gcctgacgct ctttttcccg taagccgaac cggtggttgc gaatggtggc gctgctgccg
    10861 atccccattt ccttttggat cgctgcatcg tttttcccct cataaaaaag gcgcagcagc
    10921 cggttctgat gatcggtaag cccggtcaac tgcttatcga ggcggatcag gtgttcaaag
    10981 accgagccgt gggtttgttc gatgtggacc cggatatacc tcgacgcctc ataaaaaata
    11041 ccctcccaag ggtaaacgat gcctttctcg attttttcgc cacagagcag acataaataa
    11101 tcatcgggct cttctacata accctttttg agttcctcga gggaagcatt ccaaaacagt
    11161 tcggagagat ccatgtccag ccaccttttt atgattatat aaacatctta tatggttgtt
    11221 tatcaattgt caattgcacc ggaagtaa
//
LOCUS       DASVMB010000193         8222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_688106,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000193 DASVMB010000000
VERSION     DASVMB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688106"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VF531_05865"
     CDS             <1..727
                     /locus_tag="VF531_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713527.1"
                     /translation="YGKGLYRNDSVSAASQAIAQDVVTLIVGIPLLILGLYLSGKGSL
                     KGKLLLTGTLGYFLYTYTSYSFLAMYNPFFLIYVILMSASFFAFTLAMLSFDVGKLSA
                     DFGPKLPVKFIGGFSIFLGVIIALMWLGRIQPSLVSGVPPLGLEHYTTLIIQALDLGF
                     IVPAALVAGVLVIRRKPFGYLLASVIIIKGITLLTAISAMLIGQIRAGIKVGPAETII
                     FPIFNVIAIYCLVLILKNVRGRT"
     gene            complement(855..1628)
                     /locus_tag="VF531_05870"
     CDS             complement(855..1628)
                     /locus_tag="VF531_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX7713528.1"
                     /translation="MADIIDYKIHGDDMQLVEVELDPGEGVRAEAGAMTFMEDGIIMQ
                     TSTGGGLFNGFKRMLTGDSFFITTFLNNGTGKRHVAFGAPYPGKIVPLHLNNLDGAFL
                     CQKDSFLCAAQGIEIEVAFTRKIGAGLFGGEGFILQRLQGDGLAFIHAGGTIIERRLG
                     SGEKLRVDTGCIVGFEPSVDYDIQFVGGFKNALFGGEGIFLATLTGPGLVYLQSLPFS
                     RLADRILAAATYRRQDESSGLAGFGGGILGGLLGGDRGD"
     gene            1881..2447
                     /locus_tag="VF531_05875"
     CDS             1881..2447
                     /locus_tag="VF531_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX7713529.1"
                     /translation="MKSKVNSQPPNPRRLVLASLFTALIVVGGYLSVPLPFSPVPLAL
                     ADFFVMLSGLLLGSSLGLTSTACYLLLGALGLPVFAGGKAGLAVFLGPTGGFLIGYLV
                     AAWLIGIIATGGRTNPFKDGLALLAGNIVLHVCGVSWLVATLHLSWGKALAAGVLPFL
                     PGAVIKGIAAVILARVIRPQLSGNDRRG"
     gene            2451..3200
                     /locus_tag="VF531_05880"
     CDS             2451..3200
                     /locus_tag="VF531_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713530.1"
                     /translation="MNLLEVQDLTQIFPDGTVALRGVNLTVEQGEFLIIAGRNGSGKT
                     VLARHLNGLLLPTGGRVLFRDQPITDNLVNARMRIGLIFQDSLSQLVGQTVAEDVAFG
                     PENLGLSREEVKRRTLEALDAVGLSELAEAVPQRLSGGQQRRVAIAGVLAMKPELIVF
                     DEPFTGLDYPGVVQVLQELCKLHQNGSTLIVITHDLEKILAHADRMVIMAEGVVVEAG
                     VPAAVLAKVEQYGIKRPYGADREIGTMTWLS"
     gene            3188..3949
                     /locus_tag="VF531_05885"
     CDS             3188..3949
                     /locus_tag="VF531_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX7713531.1"
                     /translation="MAQLNIFHYSPGTSPLHRMDPRIKLGMFVLMSIAVNLISGIGGL
                     TFISAFLAVAVVLSRLPLRVLMRELRNYGWIILLILVSFALGAPNRSSSLLVLGVINF
                     DGFLHGAQFVWRLTTTMVWGLIVIGTTPLGRIRDALEWLFGRLPFFPAARIGMIFSLT
                     ISLIPMVFDQAGEVLAAQKARCVELRRNPVQRIRLFVWPFIFRILGRADEMALALEAR
                     CLSDHRTPPEFKLGPVDGWILILTTTTVVVAGMLP"
     gene            complement(4193..4531)
                     /locus_tag="VF531_05890"
     CDS             complement(4193..4531)
                     /locus_tag="VF531_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7713532.1"
                     /translation="MLPFIESVKERDPQFYQLIKGIEDLTQEESSLDPKTKMMISLAV
                     DACTGATEGVIALAGALRKMGVTDAQISEVLRLVYFAKGNTVLAASMAAFRPISAPFP
                     KGDSFPHREE"
     gene            4670..5827
                     /locus_tag="VF531_05895"
     CDS             4670..5827
                     /locus_tag="VF531_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX7713533.1"
                     /translation="MKQRLGIVVGCLILLVILFIIGNEQKKSFSIRFPGEFERQQAVW
                     LQWPSEVYEGGPRPVSPVTIRLIQALAPYIRVNLMVRNQLEIELIKGLLEAGGFSGSA
                     IHYYIVSHLSIWARDVGPIFIRDRRNHLQVVDFGFNNYSRDGDPNYIDIESRVDKRVA
                     KLLGLPAVTSSLISEGGAIESNGRGCLMVTASVALKRNPGLSRMQIAKEYQRVLGSRK
                     IIWLNKGLAEDDAITSGHINEIARFADPRTILLARVPAEDRDANPVARSSYRRLEENC
                     RILRNATDQDGQPFRIIRIPMPVTLYSEAKPNGEFPVRSYLNFFITNGAVLLPVYWRP
                     GRSPALKTMEEQVKSIFRRTFPGRRIIGIDVENVNLWGGGIHCITQPMPAS"
     gene            5944..7383
                     /locus_tag="VF531_05900"
     CDS             5944..7383
                     /locus_tag="VF531_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713534.1"
                     /translation="MDFFGRKRAVLLAVGLGTFMSGLDSSVVNMALPSMGRYFHAPLS
                     TIEWTIMSYLLVVSSLLLAYGRLGDIYGHKRIYILGIVIFTTGSLFCSLSPSVFILII
                     SRGIQAIGAGMQMATGPAIVTDSVPPQERGRALSVTAVSVAIALTLGPVVGGFLTSTF
                     GWQSIFLINLPIGVFEVFLADKSIPRYSGKTRQRFDFIGAGFVFLALVGILLPLSLVE
                     EYGWSNQYILGSIVLGLVLAAGFILWERRITYPMFDVVLFHNRLFSMSNLSALLNFMA
                     QFTIILLIPFYLQQLRGLPPATAGILYMPMPLTTMLIAPISGNLSDRMDTRYLSSAGM
                     AIVALGMGLLSTLKADSPFSFLIISLIIVGLGIGLFQTPNNSAIMGAAPQSKRGIASG
                     MLATMRNVGMVLGVAISGAIFTSSNQWLTNRFQAQGLAGTGLFRAAFEGALHITYTVG
                     AVLALLAVITSLVKGPTRPGNSHQDLDAE"
     gene            complement(7362..>8222)
                     /locus_tag="VF531_05905"
     CDS             complement(7362..>8222)
                     /locus_tag="VF531_05905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7713535.1"
                     /translation="KLFHARARILLAVVTLVFTLLPVCAATLPQTGATAAVLMNARTG
                     KLLYAKNSDRTMAPASTTKIMTAILVLEHLPLDRVVKIQSSAASKEGSSMYLVAKEQK
                     TVRELLYGLLLVSGNDAATALAQAVSGTEANFAKLMNKKAALLGMKNSHFLNASGLPA
                     KGHYSTAYDLALLTRYAMQNGNFAKIVSTKVKEVPGAKAGQTRRLINHNKLLWRYQYT
                     TGVKTGYTIAAGGCLVSTASHDGVSLISVVLKSSYIYDDSQKLLSYGFKQSSRTGSIA
                     ATTDHSASKS"
BASE COUNT         1748 a         2033 c         2297 g         2144 t
ORIGIN      
        1 atacggaaaa ggcttgtaca ggaatgattc cgtgtctgcg gcatcccagg ccattgccca
       61 ggatgtggtg accttaatcg tgggaattcc cttactcatc ttggggctct acctgtccgg
      121 gaaaggatcg ttaaagggta aactgttact cacggggaca ctgggatatt ttttgtacac
      181 ctatacatct tattcgtttc tggccatgta caacccgttc tttctgatat acgtcatcct
      241 gatgtccgcc agtttcttcg cgtttaccct ggctatgctt tcgtttgatg tagggaagtt
      301 gagtgcggat tttggcccca aacttccagt gaaatttatc ggcgggttct ccattttcct
      361 cggtgtcatt atcgccctga tgtggctggg aagaatccag ccgtcactcg tcagcggcgt
      421 gccgccgctt gggttggaac actatacgac tttgataatt caggccctgg acttagggtt
      481 tatcgtacct gcggctctag tcgccggagt cctggtgatt cggagaaagc ctttcggcta
      541 tctcctggct tcggtgatca ttattaaggg gataaccttg ttgaccgcga tcagcgccat
      601 gctgatcgga cagatccgtg ccgggatcaa agtcggaccg gcggagacga tcatctttcc
      661 gatctttaac gtaatcgcta tctactgcct ggttcttatt ttgaaaaacg ttcgggggag
      721 aacctgagcc ctcacacccc tcagccgaca tccgggcctc gcgtacacag tacgcgcgcc
      781 ctctcccata gcaagtgctt ttcgggcgcg atgggagagg gccgctaact tgaatctgcc
      841 tcatgggtga gggctcaatc gccgcgatcc ccgcccaaaa gcccgcctag gattccaccg
      901 ccaaatcccg ccaaaccgct ggattcgtct tgccgcctgt aggtggctgc agccagaatt
      961 cgatcggcca atctcgagaa ggggaggctt tgcaggtaga ccaggccggg gccggtcaag
     1021 gtcgccaaaa agattccctc tcctccgaat aaggcgtttt tgaagccccc gacaaattga
     1081 atgtcataat cgactgacgg ctcaaaaccc acgatgcatc cggtatcgac ccgtaatttc
     1141 tcaccagagc cgaggcggcg ttcgatgatg gtcccgccgg catgaatgaa ggccaacccg
     1201 tctccttgca agcgctgtaa tataaaaccc tcgccgccga agagaccggc gccgatctta
     1261 cgggtaaatg ccacttcaat ctcgatgccc tgggctgcac agagaaacga gtctttttgg
     1321 cagagaaacg ctccatccag attgtttaaa tgcaatggga cgattttgcc ggggtaggga
     1381 gcgccgaagg cgacgtgacg tttcccggta ccattgttca gaaaggtagt gatgaagaaa
     1441 ctatccccgg tcaacatccg tttaaacccg ttgaatagtc cgccaccggt tgaggtttgc
     1501 attatgatcc catcttccat gaacgtcatg gctccggcct cggcccgaac accctcgccg
     1561 gggtcgagtt ccacctcgac cagctgcatg tcatctccgt gaatcttata atcgataatg
     1621 tctgccatga aaatcctcct cggaattttg atgtttctac catgtttggt tatgttatga
     1681 tacgtatttt agccctcgga gttcccgatt gcaagagtgg gttgacttta aattgtaaac
     1741 caattattat atatatggtt gacaattggt gcttttgagt ataggatccg tggttgatag
     1801 ggattgacat acccatgttt ctcttcaatg gcatggatca agttttatga agacaaaaca
     1861 tatgaaaagg atgattcctc atgaaatcca aggttaatag ccaaccaccc aatccgcgaa
     1921 ggcttgtttt ggcatcgctc ttcacggctc taatcgtggt cggggggtat ctcagcgttc
     1981 cgttgccatt tagtccggta ccgctggcac ttgcggactt ttttgtcatg ctttccggtt
     2041 tattgcttgg cagttccctg ggactcacca gtaccgcttg ttacttactc ttgggtgctc
     2101 ttggattacc ggtcttcgcc ggcggcaagg ccggacttgc tgtgttttta ggtcctaccg
     2161 gagggttctt aatcggttat ctggttgcag cctggcttat cgggatcatc gccacgggtg
     2221 gccggacgaa tccttttaag gatggtttgg ccctcttggc cggtaatata gttttacacg
     2281 tttgtggagt atcctggtta gtggcaacgt tgcatctttc atggggaaag gctctagccg
     2341 ccggggtgtt gccgttttta ccgggcgcgg tcatcaaggg aattgccgcg gtgattttag
     2401 cccgggtgat tcggccgcag ttatccggaa acgatcgtag ggggtgaatc atgaacctcc
     2461 ttgaagtgca agatctcacc caaatatttc ccgacgggac ggtggcgctc cgtggggtca
     2521 acctaacggt ggaacaaggt gaattcttaa tcattgccgg aaggaacggt tccgggaaaa
     2581 cagtactggc ccggcattta aacgggttac tgctgcctac gggggggcgg gtgcttttcc
     2641 gggaccaacc catcaccgac aatctagtga atgcccggat gcgaatcggt ctgatctttc
     2701 aagactccct cagtcagttg gtaggtcaga ccgtggccga ggatgtggcc ttcggccctg
     2761 agaatctggg gctttcccgg gaggaggtta aacgccggac tcttgaggct ttggatgctg
     2821 tcggtttgag cgaattggcg gaggccgttc cccagcggct ctccggcggc caacagcgcc
     2881 gggtggcgat tgccggagtg ctggcgatga aaccggagtt gatcgtgttt gacgaaccct
     2941 ttaccggatt ggactatcct ggtgtcgtcc aagtcttaca agaactgtgc aaacttcatc
     3001 aaaacggttc tacgctcata gtcatcaccc atgacctgga aaagatcttg gcccatgccg
     3061 accggatggt gatcatggct gagggcgtgg tggtagaggc aggcgtacct gccgcagtcc
     3121 ttgctaaagt ggaacaatac ggcatcaagc gaccttacgg cgcggatcgg gaaatcggga
     3181 cgatgacatg gctcagttga atatctttca ttattctccg ggaacttcgc ccttacaccg
     3241 gatggatccc cggatcaagc tgggaatgtt tgttttgatg agtattgcgg taaatttgat
     3301 ctccggaatt gggggcctaa cattcatcag cgcgttttta gccgtagcgg ttgttctttc
     3361 caggctgcca ttaagagttc tgatgcggga gttacgcaat tacggttgga tcattctttt
     3421 gatcctggtc agtttcgctc tgggagcgcc taaccggtca tcatcgctgc ttgttctcgg
     3481 agtaatcaat ttcgatggtt ttcttcacgg cgcccagttc gtttggcggt tgacgacgac
     3541 tatggtttgg ggcttaatcg tgattggcac tactccgctt ggtagaattc gggacgcttt
     3601 ggaatggttg ttcggacggt taccgttctt cccggcggct cgaatcggta tgatattcag
     3661 tctgacgatt agtcttatcc cgatggtatt tgatcaggcc ggggaagttc tcgcggcgca
     3721 gaaagcgcgc tgtgtagagc tgaggaggaa tccggtgcaa cgaatccggc tgttcgtttg
     3781 gccgtttatc tttcgtatcc tggggcgtgc ggatgaaatg gccctggcgc tcgaggcacg
     3841 ctgcttatcg gatcaccgga ccccacccga gttcaagttg ggtccggtgg atggatggat
     3901 cttgatcttg accaccacca cagtcgtggt ggcgggaatg ctgccgtgaa accctcaccc
     3961 ctcagccaac atccgggtct cgcgtacacg gtacgcgcgc tctcccccat catgctatca
     4021 aaaacgacag ctatggggga gggaccgaag atgtcaacct acctcttgcc cctggcaggg
     4081 aggagtaacc gtattgcttt gcagatatct ttgaaacctt tgggatactc ggacattaac
     4141 cttgttactc gaataaagat taagatttta gattagcttc aaagctcaag ggttactctt
     4201 ccctgtgagg gaaagagtcg ccttttggaa aaggcgcgga gataggtcgg aaagccgcca
     4261 tggaagcggc cagtaccgta ttgcccttgg caaaataaac caggcgcagc acttcactga
     4321 tctgggcatc ggtaactccc atcttgcgaa gggccccggc aagtgctatg actccttcgg
     4381 tcgcaccggt gcaggcatca acggccagtg aaatcatcat ttttgtcttt ggatccaggc
     4441 tgctctcctc ctgggttaaa tcctcgattc ccttgattaa ttgataaaat tggggatcgc
     4501 gctccttgac ggactcgata aatggtaaca ttgcaatcgt ctccttcttt tattccgtga
     4561 tagccaaaat gatttccatt ttgaattata atatatcccg gcgactttgt ttaccgtaaa
     4621 aaagggaata ctacttcgaa tcacgaggac gggggaataa agggatcgaa tgaaacaacg
     4681 tttgggaatt gttgtcgggt gtctcatcct tttggttatt ttattcatca tcggaaatga
     4741 acaaaaaaag tcattttcta ttcgctttcc gggtgagttt gaaaggcaac aagcggtctg
     4801 gttgcaatgg ccctcggagg tttatgaggg aggcccccgc ccggtaagtc cggtgacgat
     4861 tcggctgatt caggcgttag ccccttatat ccgggtcaac ctaatggtgc ggaatcagtt
     4921 ggagatcgag ctaattaagg ggttactcga ggccggtggt ttttccggtt ccgccattca
     4981 ttactatatc gtcagccact tatccatttg ggcgcgggat gtggggccga tcttcatcag
     5041 ggataggcga aaccatctgc aagtcgtgga tttcggcttc aataattaca gccgggacgg
     5101 cgatcccaat tatatcgata ttgaaagccg ggtagataaa cgggttgcaa agttactggg
     5161 gctgccggcc gtcacttcca gcctgatctc ggaaggaggc gccatcgagt ccaacggcag
     5221 ggggtgttta atggtgacgg cctcagtggc gcttaaacgt aatccgggat tgagccggat
     5281 gcagatcgcc aaggaatatc aaagggtgct cggttcgcgt aaaatcatct ggctaaacaa
     5341 aggattggcg gaagatgacg ccatcaccag cgggcacatt aacgagatcg cccgttttgc
     5401 cgatccccgc acgattctat tggcccgggt cccggcggag gatcgggacg ccaacccggt
     5461 tgcccggagt tcgtatcgga gattggagga aaactgccgg attttacgga acgcaaccga
     5521 ccaggacggc caaccgttcc gaatcatccg gatcccgatg ccggtgacgc tttacagtga
     5581 ggccaaacca aacggcgaat ttccggtacg cagctactta aacttcttta tcactaacgg
     5641 tgcggtatta ttaccagttt attggaggcc gggacgttct ccggctctga aaaccatgga
     5701 ggaacaggtt aaaagcatat tcaggcggac tttccccgga cgccggatca tcggcattga
     5761 tgtggagaac gtcaacctgt ggggaggagg gattcactgc attacgcagc ctatgcctgc
     5821 cagttagggc cgggattgcc tctattgttg tcccatattt gcctggatga aagcgataaa
     5881 gtctcgggga aaatatggta taatgtccga ggtaatgagg ctgatatcac ggagggcgta
     5941 caaatggatt tcttcggaag aaaacgagcc gtcttgctgg cggtgggctt aggaactttt
     6001 atgtcgggac tggattccag cgtggtcaat atggcattgc ccagcatggg ccgatacttt
     6061 cacgcaccgc tttcgaccat cgagtggacg atcatgtcgt acctgctggt ggtgagcagc
     6121 ttactcctgg catacggacg actcggagat atatacggtc ataagcgcat ttatatcctg
     6181 ggtatcgtca ttttcacgac aggttcgctc ttttgcagcc tttcccccag cgttttcata
     6241 ttgattatct cccggggaat tcaggcgatt ggtgccggaa tgcagatggc gacgggaccg
     6301 gccatagtca ccgattcggt accgccgcag gaacggggta gagctttaag cgtaaccgcg
     6361 gtatcggtag ccatcgccct gactttgggg ccggttgtgg gcggctttct tacatcgact
     6421 ttcggctggc agagtatttt tctgatcaac cttcccatcg gggtatttga ggtatttttg
     6481 gcggacaaga gtattccccg gtatagcggg aagacccggc agcgtttcga ttttatcgga
     6541 gcggggtttg tgtttctggc gctggtggga atcttgcttc cgctgagttt agtcgaggaa
     6601 tatggctgga gtaatcaata tattttaggt tcgattgtct tgggattggt tttggcggcc
     6661 ggttttatcc tttgggagcg gagaatcacc tatccgatgt ttgatgtcgt actctttcat
     6721 aaccggcttt tttccatgag caatctttcg gcgctgttaa acttcatggc acagttcacc
     6781 atcatattgc tgatcccgtt ttatctccag caattacgcg gactaccgcc ggccacggcc
     6841 ggaatactct atatgcccat gccgctcacg acgatgctca tcgcccccat tagcggcaat
     6901 ctatcggatc gcatggatac ccgttacctc agttcggcgg gtatggccat agtggcgctg
     6961 ggaatgggtc tcttgagcac cttaaaagcg gattctccgt tttcattcct catcataagc
     7021 ctgatcattg tcggtttagg gatcggacta ttccagactc cgaataacag tgcgattatg
     7081 ggggcagcac ctcagtccaa acggggaatc gcctccggga tgctggctac catgcgtaat
     7141 gtaggcatgg tgcttggcgt ggccatatcc ggggcgatct tcacctcgag caatcaatgg
     7201 ctgaccaacc gtttccaggc gcaagggctg gccggtaccg ggttatttcg ggctgctttt
     7261 gaaggcgcat tgcatatcac ctatacagtc ggagcggtgc tggctctgct ggcggtgatt
     7321 acctcactcg tcaaggggcc tacccggccc ggcaacagtc atcaggactt ggacgcggaa
     7381 tgatcggtcg tggccgcaat cgaaccggtt ctggaggatt gtttgaatcc gtaactcaga
     7441 agtttttggg agtcatcata gatgtaactt gattttagca ctaccgagat gagtgacacc
     7501 ccgtcgtggc ttgccgttga tacgagacac cctccggcgg cgatggtata tccggtttta
     7561 actccggtgg tatattgata acgccacagg agtttattat gattgatcaa acgccgtgtt
     7621 tgtcccgcct tggctccggg gacctctttc accttggtgc tgacgatctt ggcgaagttt
     7681 ccgttttgca tggcgtagcg ggttagaagg gccaggtcgt aagcggtcga gtaatggcct
     7741 ttagccggca gcccgctggc atttagaaaa tggctgtttt tcatgcccag aagtgctgct
     7801 ttcttattca tcaatttggc gaagttcgct tcggttccgg agaccgcttg agctagggcg
     7861 gtggcagcgt cgttaccgga gaccaggagc agaccgtaga ggagttcacg gacggtcttt
     7921 tgttctttgg ctaccagata catggaagac ccttccttgg aagcggccga tgattgaatc
     7981 tttaccaccc gatccagcgg gagatgctcc aggaccagga tggcggtcat gattttggtg
     8041 gtgctggccg gcgccatcgt gcggtcggaa ttcttcgcgt aaagtagctt tccggttcgg
     8101 gcattcatca gtactgccgc ggtggcgcct gtttggggga gggtcgcggc gcatacaggg
     8161 aggagcgtga agaccagagt cacaactgcc aggagaatgc gcgcgcgcgc gtggaagagc
     8221 tt
//
LOCUS       DASVMB010000194        10323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_318269,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000194 DASVMB010000000
VERSION     DASVMB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318269"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VF531_05910"
     CDS             <1..1351
                     /locus_tag="VF531_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7713536.1"
                     /translation="LPATRELGSVLKVSRNTVILAYQYLEDDGFIRIVKNQGAFVAPV
                     DVAPKKATSRDGLRLDWSQRLTDLARLATELDIEKKELKWRRGMISFKSIAPDGSLFE
                     TGEFKKAFLNRLALEGQPLLNYGYAKGYKPFIEYLFDYMSSKGVNPAGKELLITNGFT
                     EAFNLVLSALTRPGDAIICENPTHNTALKIMKLHGLDITGIPLCDDGIDPVKLEQTLA
                     QRNFRLGYVIPSYHNPTGIVMPLEKRLAVLDSFTRHQVPLLEDGFNEELKFSGSHVAP
                     LLALGGTGNSLIYLGSFSKVLFPGLRIGWIMCDAELISYLESMKRSANIHTSALDQAL
                     LYEYLQGGHFETYLKKVRRVYKERYERAVELATEFIPCKKIWGQGGLHIFIELEKMDA
                     REVLAACYNRGVVFMPGDIFFTDGGGRNTFRLGIGRVRPEEMETGFRIIGEVIRSML"
     gene            1428..2648
                     /locus_tag="VF531_05915"
     CDS             1428..2648
                     /locus_tag="VF531_05915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7713537.1"
                     /translation="MERTFKKSAKLDNVCYDIRGPVLDEAKRMEQDGQQILKLNIGNP
                     APFGFEAPEEIVQDMMANLRHAQGYSDSKGIFPARKAIMQYCQEKDIPGVGVEDIYIG
                     NGVSELIVQAMQGLLDSGDEILIPAPDYPLWTAAVNLAGGTAVHYRCDEEQNWYPDVS
                     DIRKKITPNTKGIVVINPNNPTGAVYPREVLEQIARLAAANGLIIFADEIYDRILYDG
                     VEHTSIASLTDEVLCITMNGLSKSHRIAGYRAGWMVLSGNKNSAKGYIEGLNMLASMR
                     LCSNVPSQFAIQTALGGYQSIQDLTKPGGRLHEQRDYSYQRVNQIPGLSCVKPEGAFY
                     FFPKVDVKRFNIHDDEKFMLDLLKAERVLLVQGTGFNWPEPDHFRVVFLPEMNVLKNS
                     LERLEHFLGSYQQS"
     gene            complement(2786..4045)
                     /gene="ylbJ"
                     /locus_tag="VF531_05920"
     CDS             complement(2786..4045)
                     /gene="ylbJ"
                     /locus_tag="VF531_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HEX7713538.1"
                     /translation="MRIFQVKKVNIAVLKKVNVLLGAALVTLLTVTLAVYPKESVEAA
                     TAGLKIFWEIVLPSLLPFFVLSEIMLGMGVVHFIGVLLEPLMRPVFNVPGAGAFALSM
                     GLAAGYPMDAVITGKFRRNGMCTQVEGERLLSFTNTADPLFIFGAVAVGMFGRPQLGV
                     ILAIAHYLSSFTVGFIYRYYRRDEVQTHTATGSNRKILPQAVRALLQARREDGRPFGK
                     IMGDAVRESMSTLLMIGGFIMIFSVLVRILAAIGVLQIINPILASILKLFGLNPILAQ
                     AVFNGILEIDLGTMAASQVNAALIDRTMVASWIIGWSGLCVHGQVASVVHDTDIRMGP
                     YVAGRLLHGLIAAIYTGFLMGPLFPVFAPFARLAPVTLKFNPLDRILLSGVELLIGFG
                     VFILAALVVFLCRRYRLVRMETEKSPS"
     gene            complement(4485..5405)
                     /locus_tag="VF531_05925"
     CDS             complement(4485..5405)
                     /locus_tag="VF531_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7713539.1"
                     /translation="MIYDLAIIGAGPSGMTAGVFAARKGLKTIIISKDIGGQVNWTTD
                     IENYMGFQEIDGQALVDRFEEQVKDLHLEEKLTSVESLGKEGELFKMKTAAGEEILAR
                     AVIVSSGKKPRTLNIPGETALRNKGVSYCATCDGPLFRDLDVAVVGGGNSAVKSALDL
                     AHYAKFVHLFAISELTADAILTDRLKQLPNLKIYTGHTVVEIQGEKSVERIVAEVAAT
                     KQRFTIPLQGVFIEIGLETNVSFIDRGVERNEANEIKVDCLCRTNISGLYAAGDVTSV
                     PEKQIIVAAGEGAKAALKAWDYLMEHQYQV"
     gene            complement(5561..5791)
                     /locus_tag="VF531_05930"
     CDS             complement(5561..5791)
                     /locus_tag="VF531_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7713540.1"
                     /translation="MSEITVFTTPTCPWCSRVKAYLHDKGVSFDEVNVASDRLGAERM
                     IALTGQRSVPVIAKGNQYVVGFDPDRLEGFIH"
     gene            complement(5859..6965)
                     /locus_tag="VF531_05935"
     CDS             complement(5859..6965)
                     /locus_tag="VF531_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713541.1"
                     /translation="MAKLLRLTIALAILGAMAAGIVRAAPDQDFLVFYQEDAGRKTVY
                     LSPENSADLQTVASGTKLDVFLLPRHLVYYTGHRLYEYSLDQRKVKPLAQLDAAETLY
                     QVLAYDSEGPDQAFVITSDEFGTIHQYVMEFSDGSLRRIDGPESNLGSGLSATGTEPQ
                     VLSPDGAAAIRIHQVPFSSRLEIRLVPMKSGSTLKKWTLTSQWTTLPQFPIWSPDSKY
                     FAFYARHSNQSTGFYDLYLFNLESARMELVQSQVFTRLPFEDRVMGEFTPSWSPDSRY
                     LIFEYQPYGLPTESSILRYEAGSGKRFTITGGLGQRRTPVWSPSGRKLFFIMDAAGTG
                     PQVHWINADGTGERRVSPSGGTTVWAEWYQPNRG"
     gene            complement(7022..8050)
                     /gene="hypE"
                     /locus_tag="VF531_05940"
     CDS             complement(7022..8050)
                     /gene="hypE"
                     /locus_tag="VF531_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX7713542.1"
                     /translation="MRVLKQLPEKIRLAEGGGGEATANLIREVFWSRLANPELLRGND
                     AAVLKNPGGRLALTTDSFVITPWRFPGGDIGRLAICGTINDLTVMGAKVLGLAAAFII
                     EEGFSADSLTEISQSLANTAREAGVFVVTGDTKVVNRGAADGIFITTTGLGVIDEGIV
                     LGGEMARPGDRVLISGTMGDHGAAVMLARGELGIESGLQSDAAPLNSLLLPLIEEYHD
                     SLRVMRDPTRGGLAATLNEIARQSQVGIRLDEKVIPVRPEVRGITDLLGLDPLYLANE
                     GKAIIVAAPQIAEELVTRLHRHPLGQAAAIIGEVTAAHPGMVSLQTTAGGERILLMGS
                     GEQLPRIC"
     gene            complement(8059..9159)
                     /gene="hypD"
                     /locus_tag="VF531_05945"
     CDS             complement(8059..9159)
                     /gene="hypD"
                     /locus_tag="VF531_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX7713543.1"
                     /translation="MSNSSINSFNQGTENLHSLIKKVQTSYPDLRATLMEVCGTHTNA
                     IRRAGIHRMLPSGVRLLSGPGCPVCVTGSGFIEQAIRLSHRPRTIVASFGDLLKVPGN
                     SGTLSEARAAGGAIRIVYSPLDAVNMARENPDREVVFLGVGFETTAPTVALAIREAME
                     RKLANFSVLTAFKILDPALRCLLKDQELQINGLIAPGHLAVILGANAFTFLPAEFRLP
                     TVVTGFQPVEIWRGIGALLNQIAHGEARLENAYAPLVSPEGNIAAQRLLADLFTPEPA
                     EWRGLGAIDGSGLGLRDEYRKYDAASRFGLMPIVSQEPPGCQCGRILLGKAEPEDCVH
                     FGRSCTPEKAIGPCMVSAEGTCAAHFSYRGFE"
     gene            complement(9156..9368)
                     /locus_tag="VF531_05950"
     CDS             complement(9156..9368)
                     /locus_tag="VF531_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX7713544.1"
                     /translation="MCLAIPGEVTVIDGNSATVSFSGALRKADLRLVEGVQVGDYVLV
                     HAGCAIQVIPKAEALETLRLWSEISE"
     gene            complement(9608..>10323)
                     /locus_tag="VF531_05955"
     CDS             complement(9608..>10323)
                     /locus_tag="VF531_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX7713545.1"
                     /translation="EFFAGEGCHWERLGHLEYTPLPGGEAAVREPWRMAVTYLRAANP
                     EWAASWAGGQGLELPWAKLEAARQLGINHPETSSMGRLFDGIAVLCGAPHRVSYEGEA
                     AIWLENQADPSEPGRYDWEIADSDGELIVRLAPLISGIYRQLKTGVKGSVISSRFHRT
                     IAALTVAMVSRITVNSGINQVILSGGVFQNRLLLELTYAQLDAAGFRVIIPEILPIND
                     GGVALGQAWFGALAILEGQ"
BASE COUNT         2510 a         2940 c         2600 g         2273 t
ORIGIN      
        1 cctgccggcg acccgcgagt tgggcagcgt gctcaaggta agccgcaata cggtgattct
       61 ggcctaccag tacctggagg acgatggctt tatccggatc gtcaagaatc agggagcatt
      121 cgttgccccg gtcgatgtgg ccccaaagaa ggcgacttct agagacgggt taaggcttga
      181 ctggtcacag cgccttacgg acctggcgcg gctcgctacg gaactggaca ttgaaaagaa
      241 ggaattgaaa tggcggcggg gcatgatctc ctttaaaagc atcgccccgg acggttcgct
      301 cttcgaaacc ggggagttta aaaaggcctt tctaaaccgg ttggcgctgg aaggtcaacc
      361 cctattaaac tacggctacg ctaaaggata caaaccgttc atcgaatatc tctttgatta
      421 catgtccagc aagggagtga acccggcggg caaggaactc ctgatcacca acggttttac
      481 cgaggctttc aaccttgtcc tgtctgcact cacccgtccc ggtgacgcca taatctgtga
      541 aaaccccact cacaatacgg cactaaagat aatgaagcta catggactgg atatcaccgg
      601 aatccccctc tgcgatgacg gaatagaccc agttaagcta gagcagactc tggcacagcg
      661 aaatttccgg ttggggtatg taatcccttc ttatcacaat cccaccggga tcgtgatgcc
      721 ccttgagaag cggctggcgg tcctcgatag tttcacccgc catcaagtcc cgctgctcga
      781 agacggattc aatgaagaac tgaaattctc cggctcccat gtggccccct tactggccct
      841 ggggggaaca ggaaatagtc tgatctacct gggcagtttc tccaaggttc tcttcccggg
      901 ccttcggatc ggctggatca tgtgcgatgc cgaactcatc tcatacctgg aaagcatgaa
      961 gcgaagcgcc aacatccata cctccgccct ggaccaggcc ctgctctacg aatatttgca
     1021 gggcggacat ttcgaaacat acctgaaaaa ggtccgccgt gtttataaag aacgctacga
     1081 gagagctgtg gaactggcca cggaatttat cccttgtaaa aagatctggg gccagggcgg
     1141 gttgcatatc ttcatcgaac tcgaaaagat ggatgcccgg gaggttttgg ccgcctgcta
     1201 caaccgggga gtagttttta tgccgggtga catcttcttc accgatggcg ggggccgcaa
     1261 caccttccgg ttggggatag gccgggtaag gcccgaggaa atggagaccg gtttccgaat
     1321 catcggtgaa gttatccgtt caatgctgtg atataaagat caacaatcaa tcccggatag
     1381 accccttaaa aggacggaac gaccaattac gaaaggatga ttttcagatg gaacgtacct
     1441 ttaaaaaatc agccaagttg gacaatgttt gttatgatat ccgcggcccc gtgcttgatg
     1501 aggccaaacg gatggaacaa gacggccagc agatcttgaa gctgaatatc ggcaatccgg
     1561 cgccctttgg ttttgaggct ccggaggaga tcgttcagga tatgatggcc aatcttcgcc
     1621 atgcccaggg atacagcgat tccaagggga tcttcccggc ccgcaaggcg atcatgcaat
     1681 attgccaaga gaaggatatt cccggggtag gcgtcgagga catctatatc ggcaatggcg
     1741 tcagcgaatt aatcgtccag gccatgcaag gccttctgga cagcggcgat gagatcctga
     1801 ttcccgctcc cgattacccg ctctggaccg ctgccgttaa cctggccggg gggactgcgg
     1861 ttcattatcg ttgcgatgaa gagcagaact ggtatccgga tgtcagtgac atccgcaaaa
     1921 agattacccc caacaccaag ggcattgtgg tgatcaaccc caacaatccg accggggccg
     1981 tttatccccg cgaggtcctg gaacagatcg cccgattggc cgccgccaat ggcctgatca
     2041 tcttcgccga cgagatctat gaccggattc tttacgacgg agtggagcat acctccatcg
     2101 cttcgctcac cgacgaagtg ctctgcatca ccatgaacgg tttgtccaaa tcccaccgga
     2161 tcgccggtta ccgcgccgga tggatggtgc tctccggtaa caaaaactcg gccaagggtt
     2221 atatcgaagg cctgaacatg ctcgcgtcaa tgcggctctg cagtaatgtc ccgtcccagt
     2281 ttgccatcca aaccgctctc ggcggttatc aaagcatcca agatctgacc aaacccggag
     2341 gccgtctcca cgagcaacgg gactattcct accaacgggt caatcagatt cccggtcttt
     2401 cctgtgttaa acccgagggg gccttttact tcttccccaa ggtggatgtg aaacgtttca
     2461 acattcatga cgatgaaaag tttatgttag acctgctcaa agcggaaagg gttctgcttg
     2521 tacagggaac cggatttaac tggccggaac cggatcattt tcgggtggtc tttctgccgg
     2581 agatgaacgt actcaagaac tccctggaac ggctggaaca ttttctcggc agttatcaac
     2641 aaagttaatg ccctcagtta gcatccggac cagccctccc ccatctacca gccgttcatt
     2701 acggtctgtg cggaatgggg gaggggactt attcccccaa atcgaccacc ttatccggga
     2761 ctttccccaa aaaccatctt caaagttacg atggcgattt ctccgtttcc atccggacca
     2821 ggcggtaacg cctgcacaaa aacacaacca gggctgccag aatgaatacc ccgaaaccga
     2881 tgaggagctc caccccggag aggaggattc gatcgagcgg attgaacttc agcgtcaccg
     2941 gggccaatcg ggcgaacggc gcaaaaaccg ggaagagtgg tcccataaga aatccggtat
     3001 aaatcgcggc aattaaacca tgtaacagac ggcctgccac atatggaccc atccggatat
     3061 cggtatcgtg aaccacgctg gccacctggc cgtgaacgca aaggccgctc cagccaataa
     3121 tccagctggc caccatcgtt ctatcgataa gcgccgcatt aacctgactg gcagccatgg
     3181 tccccagatc aatttccaga attccgttga atacggcttg ggcgaggatg ggattcaatc
     3241 caaacagctt aagaatggag gcgagtatcg ggttgataat ctgtaaaacg ccgatcgcag
     3301 ccagaatccg gaccagcacc gaaaagatca tgataaaccc gccaatcatc aacaaggtgc
     3361 tcatcgattc acggaccgcg tcgcccatta tttttccaaa agggcgaccg tcctcacgcc
     3421 tggcttggag caatgcccga accgcctggg gcagtatttt acgattcgat ccggttgcgg
     3481 tatgagtctg aacctcgtcc cggcggtagt agcggtaaat gaatcccact gtaaaactag
     3541 agaggtaatg ggcgatggca aggattaccc ccagctgggg acggccgaac atgcccaccg
     3601 ccaccgcgcc gaagatgaag agcggatccg cggtattggt gaatgagagt aacctttcac
     3661 cctcgacctg agtacacatg ccgttcctcc tgaatttacc ggtgatcacc gcatccatgg
     3721 gatatccggc cgcaagaccc atcgacagcg caaacgcccc ggctccgggt acattgaaaa
     3781 ccggccgcat caaaggctcg agcagcaccc cgataaaatg aaccacaccc attcccaaca
     3841 tgatctcgga cagaacaaaa aacggcaaaa gagacggcaa aacgatctcc caaaagattt
     3901 tcagtccggc ggtggccgcc tccacgcttt ccttgggata aaccgccagg gtgacggtga
     3961 gcaacgtaac caaagctgct cccagcaaaa cgttgacttt ctttaatacg gcgatgttga
     4021 cttttttaac ttggaatatc cgcaccaaac cgtgccccct tccttgtgat agggagtaat
     4081 gaatcggcaa gagttcaacc tttaaaatat atggttgggg cgcagcggtt agactaacag
     4141 tccgattggt gatggatgat gagaaaaagg atttatcggt cgggggtcaa attattctta
     4201 ttaggtgaat ttttaaggag ggagtttcgc ggggtgactt tgtagccctt caaacgatca
     4261 agtggccgaa aagattaact gttttcactg caaatacttc tacatcactt gggacccgca
     4321 ttttcaaaac ggttgcaaag tattcggctt taaaacccgg atgcagcccg ccgtgctggt
     4381 ccgcgagtcg accggttcgg attgcatcgc cttcgagccc aagacaaaaa cggccgggtg
     4441 ccaatagcat ctaaggagtt tttttacact tcaaacttca ctcttcatac ttgatattga
     4501 tgctccatca aataatccca ggccttgagc gccgctttag cgccctctcc tgcggcgacg
     4561 atgatctgtt tctccggtac cgaagtcacg tcaccggcgg catatagacc ggaaatattg
     4621 gtccggcaaa ggcagtctac cttgatctcg ttggcctcgt tccgttccac cccacggtca
     4681 ataaatgaga cattcgtctc cagaccgatc tcgataaaga ctccctgtag cggtatggtg
     4741 aatcgctgct tggttgcggc aacttcggcg acgatccgtt ccaccgattt ttctccttga
     4801 atctccacga ccgtgtggcc ggtatagatt ttcaaattgg gcaattgctt caggcggtcg
     4861 gttaaaatgg catccgccgt caattcggag atcgcaaaca ggtggacgaa ttttgcgtaa
     4921 tgtgccagat ccagtgcaga cttgacagcg gaattacctc cgcccacgac ggcaacgtcg
     4981 agatcgcgaa agagcggacc atcgcaggtg gcgcagtaac ttaccccttt attcctaagc
     5041 gcggtctccc ccgggatatt caaggtacgc ggttttttcc ccgaggatac gattaccgcc
     5101 cgggccagga tctcttcccc ggccgcggtc ttcatcttaa agagttcgcc ctcttttccg
     5161 agggactcga cggaggtcag tttctcttcc aaatggagat cctttacctg ttcttcaaac
     5221 cggtcaacca gggcctggcc gtcaatctct tgaaaaccca tgtaattctc gatatccgtg
     5281 gtccaattga cttgtccgcc gatatcctta ctgataatga tggttttcaa acccttccgg
     5341 gctgcaaaga ctccggctgt cattcccgac ggaccggcgc cgatgatcgc cagatcgtaa
     5401 atcattcgtt tcactccgtt tgtctattaa ccttcacgaa agtccttact cacggacttt
     5461 cgtgaagaaa tatgaaatat ccgatatccg gctttgcaat tgtcttttca ctaataacca
     5521 gtaaccaatg aagaataact ttttgcattc ggatcgaggg ttaatgaata aacccttcca
     5581 gacgatcagg atcaaaccct accacatact ggtttccctt ggcaatcacc ggtacactgc
     5641 gttgtccggt gagtgcaatc atccgttccg ctcccaaacg gtcggaagcc acattcacct
     5701 cgtcgaagga cactccttta tcatggagat aggccttgac ccggctgcac cagggacagg
     5761 tgggagtcgt gaaaaccgta atttcactca tagaaagcgc ctccagagat ttgtaggaat
     5821 tgttttgccg gaattaatct ttcctgaagg cgacattttt atccccggtt gggctgatac
     5881 cattcagccc aaaccgtggt tcccccactc ggtgataccc tccgttcacc ggttccgtca
     5941 gcattaatcc aatgaacctg cgggcctgta cctgccgcat ccatgataaa aaagagtttt
     6001 cggccggaag gcgaccaaac cggggtacgt cgctgcccca ggcctccggt gatcgtaaac
     6061 cttttccctg atccagcctc ataacggagg atcgaacttt ccgtaggaag cccatagggc
     6121 tgatattcaa agatcagata tctgctgtcc ggcgaccacg aaggggtaaa ctcgcccatt
     6181 acccggtcct caaatggcag cctggtaaaa acctgagatt gtaccagctc catccttgca
     6241 ctctcaagat tgaaaagata caaatcgtag aatcccgtcg actgattgct gtgacgggca
     6301 taaaaagcaa aatatttcga atccggagac cagataggga actgtggcag agttgtccat
     6361 tgtgaggtta gggtccactt tttaagggtc gagccggatt tcatggggac cagccggatt
     6421 tccaaacgac tgctgaacgg tacctggtga atcctgatgg cagcagcccc atcggggcta
     6481 agaacctgtg gttcggtacc ggtcgcgctc aatccggatc ccaaattgct ctccggtcca
     6541 tcgatccggc gcaggctacc atcggagaat tccattacat actgatgaat ggtcccaaac
     6601 tcatcggaag ttattacaaa ggcttggtcc ggaccttccg aatcatatgc gagaacctgg
     6661 tataaagtct ccgccgcatc cagttgtgcc aagggtttaa cctttctctg atcgaggctg
     6721 tactcatata aacggtgccc ggtataataa accaggtgac gggggagtag gaaaacatcc
     6781 aactttgttc cgctggccac tgtttgaagg tcggcactgt tctcgggcga cagataaaca
     6841 gtcttccgac cggcgtcctc ctgataaaag accaaaaaat cttgatcagg ggcggcccgg
     6901 acaatcccgg ccgccatcgc tccgagaatc gccaatgcta tcgttaaccg aagcagttta
     6961 gccatttcgc tttctccttc accaattgat caacatgaca cacgtacctg ccggatcagg
     7021 tttagcaaat gcgcggcaac tgttcgccgc tgcccatcaa gaggatccgc tcgccgcctg
     7081 cggtggtttg aagacttacc ataccgggat gagcggcagt cacctctccg ataatggccg
     7141 cggcttgacc taacggatgg cgatgcaaac gggtcacaag ctcctcggca atctggggtg
     7201 ccgcaacgat gatggctttg ccttcattgg ccaggtataa tggatctagt cccagcagat
     7261 ccgtgatccc gcgaacctca gggcgtaccg ggataacttt ttcatccaac cggatcccga
     7321 cctgagactg acgagcaatc tcattaaggg tcgcagccag accgccccgg gtgggatcac
     7381 gcatcacccg caaggaatca tgatattcct caattaaagg caatagcagc gagttgagcg
     7441 gcgccgcatc gctttgcagt ccgctttcga ttcccaattc acccctggcc aacattactg
     7501 ccgcgccatg gtcgcccata gtgccgctga tcaaaaccct gtctccgggc cgggccatct
     7561 ccccgccaag gacgattcct tcatcaatta cacccaagcc ggtggtagtg atgaagatcc
     7621 catcggcggc gccgcgattc accaccttgg tatcgccggt aaccacgaaa accccggcct
     7681 cacgggctgt attcgccagt gactggctga tctccgtgag tgaatcggcg ctaaatcctt
     7741 cctcgataat aaaggcggcg gcaaggccca aaacttttgc gcccattacg gtcagatcat
     7801 tgatggtacc gcagatggcg agtctcccga tgtctccacc cgggaaacgc cagggcgtaa
     7861 tgacaaaaga atcggtagtc aacgccaacc tgcctccggg gttcttcaga accgcggcat
     7921 cattcccgcg aagtagctcc ggattggcca gacgcgacca gaagacctca cggatcaaat
     7981 tagcggtggc ctccccgccg ccaccttcgg caagccggat cttctccggt aattgtttca
     8041 aaaccctcac tcctgttctc attcaaatcc ccggtaagaa aagtgggcgg cgcaagtgcc
     8101 ctcggctgat accatacacg gtccgatggc cttctccggt gtgcagctcc tgccaaaatg
     8161 aacgcagtcc tcgggttccg ccttaccaag caggatccgg ccgcattggc agccgggcgg
     8221 ttcctgggaa acaatcggca tcaaaccgaa acgggatgct gcatcatact tcctgtattc
     8281 atcccgtaat cccaatccgg aaccgtcgat tgcccccaat ccccgccatt cggcgggttc
     8341 gggggtgaat aaatccgcga gtaatctttg agccgcgata ttgccctccg gagagaccaa
     8401 aggagcatag gcattctcca atctggcctc tccatgagca atctggttca atagggctcc
     8461 aattccccgc cagatctcaa ctggttgaaa cccggtaacc accgtgggta acctaaattc
     8521 cgccggcaaa aaagtgaagg cgtttgcccc gaggattacc gccagatggc cgggggcaat
     8581 cagcccgtta atctgtaatt cctgatcctt tagcaaacaa cgcagcgccg gatctaaaat
     8641 cttgaaggcg gttaaaaccg agaagttcgc aagcttgcgt tccatggcct cacggatggc
     8701 caaagccacc gtgggggcag tggtttcaaa tcccaccccc aggaaaacca cttcacggtc
     8761 gggattctct cgggccatat ttacagcgtc gagaggcgag tatacgattc gaatcgcccc
     8821 gccggcagca cgggcttcac ttagggtccc ggaattaccg gggaccttca acagatcccc
     8881 gaaactggcg acgatggttc ggggcctgtg actcaaccga atcgcctgct cgatgaaacc
     8941 gctgcctgtt acgcaaaccg ggcaaccggg gccggaaagg agccgcaccc ctgatggtaa
     9001 catccggtgg atccccgccc tgcgaatcgc gttggtgtgg gtaccgcaaa cctccataag
     9061 tgtagcccgg agatccggat atgaggtttg cacttttttg atcaaggaat gcaagttctc
     9121 cgttccctga ttgaagctgt ttatactact gttgctcatt ccgatatctc actccacagc
     9181 cgcagtgtct ccaaggcttc cgcctttggg atcacctgaa tggcgcagcc ggcatgaacc
     9241 agaacatagt caccgacctg aaccccttcg accaaccgga ggtccgcctt gcggagtgcc
     9301 ccggaaaaac tcaccgtggc cgaattccca tcaatcaccg taacctcgcc gggtatagcc
     9361 agacacatga ggatcgctcc tttgacatac agtctgaaat ttgaaagtca attaaggcaa
     9421 gaggcatgag tattgaaagt tatttatgat agtcttaagt ccgaaggcct gttggcaact
     9481 cgcccccgtc ccctctcttt caaaaaagag aggggcgtag ggtcatgcta taaacggata
     9541 aatttcgcgg ggtgagtgtt gccgcgcgcc ctgctggacg cgatacctcg cgcctcatgc
     9601 ctcacgccta ttgcccctcc agtatcgcca acgccccgaa ccaggcctgg cctagcgcta
     9661 ctccgccatc gttaatcggc aggatctccg gtataatgac acggaacccg gccgcatcca
     9721 actgagcata agttaactca agcaataaac ggttttgaaa taccccgccg cttaagatta
     9781 cctggtttat gccggagttg acggtgatgc gggacaccat cgctaccgtc aatgcggcga
     9841 tggtgcgatg aaacctactg ctaataacgg accctttaac accggttttt agctggcggt
     9901 agatcccgga gatcaaggga gctagtctca caatcaactc cccgtcgcta tcggcgatct
     9961 cccaatcata cctgccgggc tcggacggat cagcctggtt ttcaagccag atggcggcct
    10021 cgccttcgta actcacccta tgaggagcgc cgcataatac ggcgataccg tcaaagagcc
    10081 gtcccatgga ggaagtttcg gggtgattga tccccagttg ccgggcggct tcaagtttgg
    10141 cccaaggtaa ctcaagaccc tgccctccgg cccaagacgc tgcccactcg ggattggcgg
    10201 cacgtaaata ggtaaccgcc atccgccacg gttcacgtac tgccgcctcc ccacccggta
    10261 gcggcgtgta ttccagatgg cccaagcgtt cccaatggca gccttcaccg gcgaagaatt
    10321 cgc
//
LOCUS       DASVMB010000195         5908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_318329,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000195 DASVMB010000000
VERSION     DASVMB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318329"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VF531_05960"
     CDS             <1..825
                     /locus_tag="VF531_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7713546.1"
                     /translation="PDLLTQAPLNMLQAGFGDILGKYTALADWELGRVVNGEAYSAEV
                     AAEVRRAVDEVLALSDDLNTPVMAERLMRALVISGEAMLAWGNSRPASGAEHHLAHFW
                     EMRAGLTGKTHHLHGIEVGVAVTYIAELYHRVFSLNTKTVRNLIAARSVEPETDYQER
                     INWAFGPLAREVFDDLKGEYRDEAKRRSRQEAILAKWDLLRERITPLVPSAEELRRLL
                     QKHGASVAAEELGFDHSELLLALENAKEVRTRYTIFRLAEDIGIAPNGCAATGNRE"
     gene            complement(1111..1905)
                     /locus_tag="VF531_05965"
     CDS             complement(1111..1905)
                     /locus_tag="VF531_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7713547.1"
                     /translation="MKKHILVILSLVLLVGILAAGQGSAATVLKVGASPTPHAQILEL
                     IKPQLEKEGITLQIVEFQDYVQPNLALAEGELDANYFQHVPYLNQFAKDHNLDLTYIA
                     KVHIEPMGVYSRKIKSLKELSDKAIVAIPNDPTNGGRALLLLQQAKLITLRKDAGLAA
                     TELDIAKNPKHLQIKPLEAAELPRALEDVDAAVINTNYALLAKLVPTKDALVMEGSQS
                     PYANILAVRTKDKNNPALKKLAKALTSKVVKDYLLETYKGSIVPAF"
     gene            complement(2162..2815)
                     /locus_tag="VF531_05970"
     CDS             complement(2162..2815)
                     /locus_tag="VF531_05970"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEX7713548.1"
                     /translation="MNPQLWTLLIKALWETIYMVAISLALSAVFGIPLGILLVITGPQ
                     HIRPNPAFHNVLATIVNIGRSLPFIILLVAIIPFTRLVVGTSIGTAGAMVPLTIGAIP
                     FLARVVESAVLEVDWGVIEAAQAMGATTWQIITKVLIPEARSGLILGLTITAVSLVSY
                     SAMAGTVGGGGLGDLAIRFGYQRFQADVMLATILVLIVLVQGMQSLGDWMAKRLNRR"
     gene            complement(2784..3845)
                     /locus_tag="VF531_05975"
     CDS             complement(2784..3845)
                     /locus_tag="VF531_05975"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEX7713549.1"
                     /translation="MIRLNHITKIYEDRDNRVVALNDVSLEIQKGEIFGIIGLSGAGK
                     STLIRCINRLEVPQSGTIEIANREMTALNGKELREARREIGMIFQHFNLLATRTVFGN
                     VAFPLEIAGMPKDEIRERVKELVELVGLADKLNAYPSQLSGGQKQRVGIARALANRPK
                     VLLCDEATSALDPETTKSILKLLQDLNRRLELTIVLITHEINVIKEICDNVAVIEDGR
                     IIEAGPVVELFTHPRTNTARGFIKSVLHTEIPEEIKQRKIILHRAGQIGKTVHISFIG
                     SVAGEPVITSLIRQFNVDVNIIYANLDFIKDNPFGSLVVEVVGTDANVTAGLQYLLEQ
                     GLKIEVLNGVEPSTLDTAH"
     gene            4255..4587
                     /locus_tag="VF531_05980"
     CDS             4255..4587
                     /locus_tag="VF531_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7713550.1"
                     /translation="MDPQIKKGILDVIVLSLLRKEDTYGYLLSVQVAQMVAIAETALY
                     PVLRRLEAQGLLETYSVEYGGRLRKYYRITAAGITRLNQSLDELLELKELIDKIMEGV
                     EDHGGTNR"
     gene            4568..5833
                     /locus_tag="VF531_05985"
     CDS             4568..5833
                     /locus_tag="VF531_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEX7713551.1"
                     /translation="MAERTAEFIQSVREGLADLPADLTQGALDYYEEFIAEALESGIS
                     ETEVLKRLGRATDVVAAIRAERFIDRAEQRPGPLGLIQASGKAFGGYAFSAGRLLLAI
                     GPLCMVLIGGMFYITAVLSWIGALASIPVILSSITQIVPEYWTGKLSLVGTGFIAFSL
                     LAGTGVGFWQAADWMTKLTMRWLRRLIQPRPNRKPQPVRNESEKMKPGMKILLRVCCG
                     LFLAGVPLLFSSDLPVNCFTIWNSWVPGDLTSSTREFVPEEIRRITINTLNSRIEIEP
                     ADIAVIKLTYGQPEWMDCTLETRGGELQFTEKSAGKIPYMNFIARHEGMTVIRLEVPR
                     RYAADAVELVSIGGHMILDLRAESIQASTGDGNIRYRSRGPGEVIRAGTQRGSIWVRG
                     AVVGKLTFERGKSGRPVVLWSDGGKIEIE"
BASE COUNT         1380 a         1414 c         1666 g         1448 t
ORIGIN      
        1 cccgatcttc tgacacaggc ccccctcaat atgctgcagg ccggtttcgg tgacatcttg
       61 ggtaaataca ccgccttggc cgattgggag ttggggcggg ttgttaatgg tgaagcatat
      121 tctgccgagg tggcggccga agttcgccgg gctgtcgatg aggtgttggc cttaagcgat
      181 gatttgaata ctccggtgat ggctgaaaga ttgatgagag ccttggttat ctctggagag
      241 gccatgcttg cgtggggaaa ttcgcggccc gcatccggcg ccgaacatca tctggcccat
      301 ttctgggaga tgcgcgcggg tctgaccggg aagactcatc atctgcatgg gattgaagta
      361 ggtgtggctg ttacttatat cgccgaactt taccatcggg tgttcagcct aaacaccaaa
      421 acagtgcgaa atctgattgc agcccgatcc gtggaaccgg agacagatta ccaagaaagg
      481 attaactggg cttttggacc tctggcccgt gaagtattcg acgatttgaa aggtgagtac
      541 cgggatgagg cgaagcgccg tagccggcag gaagcgatcc tggccaaatg ggatctattg
      601 cgggagcgaa ttacgccgct tgttccatct gctgaagaac tcaggcggtt gctgcagaaa
      661 cacggggctt cagtcgcagc ggaagagttg ggttttgacc attccgagct tttactggca
      721 cttgagaatg ccaaagaggt caggacccga tacaccatct tccggctggc cgaggatatc
      781 ggcatcgcgc ccaatgggtg tgcggcaaca ggcaataggg aataggcaac gggcctgccc
      841 tcacccctca gctcattcaa agatgccggc cctctcccgt tatcacgggc gagggtttat
      901 gaatgcctat ttttaaaccc tcgaattcac cgtcctgatt cttcccacgc aatcgcgggg
      961 gaaggcgcaa gtgcacggtg caagcgacat ccttgagtta gccccagtga tgagggactt
     1021 tgaaaaatga attttcgtat ttacgcaaaa ataaagggct gaccccggtt gggagtcagc
     1081 catctgtata tttggtatat gctttattgt ttaaaacgcc gggacaatgg agcctttgta
     1141 ggtctccagg agataatcct tcacgacctt gctggtcaaa gccttcgcca gtttcttcag
     1201 ggccgggtta ttcttgtcct tggtccgcac cgccaggatg ttggcgtagg gcgattggct
     1261 gccttccata accagggcgt ccttggtggg gaccagcttg gcaaggagcg cataattggt
     1321 gttgattaca gccgcatcca catcctcaag ggcccggggc aattctgccg cttccagagg
     1381 tttgatctga agatgtttgg ggtttttggc gatgtcgagc tcggtcgctg ccagtcctgc
     1441 atccttacgt aaggtgatca atttggcctg ttgcaggaga agcagagcac ggccgccatt
     1501 ggtgggatca ttgggaatgg cgacgatggc tttatcagac aattctttca aggatttaat
     1561 ctttcgggaa tatactccca tcggttcgat gtgtactttg gcgatataag taagatccag
     1621 attgtgatcc ttagcaaatt ggtttagata cggaacatgt tgaaaatagt tggcgtccag
     1681 ttcgccttcg gccagagcca gattgggctg gacatagtct tggaactcca caatctgcag
     1741 ggtgatgcct tccttttcaa gttgaggttt gataagttcc aggatctgag catgcggcgt
     1801 gggcgatgct cccaccttga gtacggtggc agccgaacct tggccggcag caagtattcc
     1861 gacgagcagg accagggaaa ggataaccag gatatgtttt ttcattatgg ggcctcctta
     1921 aatgagtgtt ttggttgttg ttgagtagca atacctactc acagatgcaa aggtatccgc
     1981 atgttcattg catcgtcatg atgcaagcgt gtccgccttc tccggtggct ttactgacta
     2041 ctttcgaaca ccttttcatc ttgatcagcc cctaacttaa gaacgtcttg acgtccttac
     2101 tttattcaac ggcaacaggc tttttgactg ttgccgattg cctgttgcct aataccttac
     2161 gctaccgtcg gttcaatctt ttggccatcc agtcgccgag ggattgcatt ccttggacca
     2221 agactatcaa aaccaggatg gtggcgagca tcacatcggc ctggaacctc tggtagccga
     2281 aacggatggc caaatctccc aggccgccgc cgccgacagt gccggccatg gccgaataac
     2341 taaccagact gaccgcggtg atggtcaacc ctaaaattaa cccggaacgg gcctcgggga
     2401 ttagaacctt ggtgatgatc tgccaggtgg tcgcgcccat ggcctgggcc gcttcgatga
     2461 ctccccaatc cacttccaat acggccgatt caacaacccg cgccaagaaa ggaatcgccc
     2521 cgatggtcag gggcaccatg gcaccggctg tgccgatgga ggtgccgacc accaagcggg
     2581 tgaagggaat gatggccacc agcaagatga tgaacggcag ggaacggccg atgttgacga
     2641 tagtcgccag gacattatga aaggccggat tgggccggat atgctgcggc ccggttatta
     2701 ccaagagtat accgagaggt atgccgaaaa cggctgaaag cgccagagag attgccacca
     2761 tataaatggt ttcccagagg gccttaatga gcagtgtcca gagttgaggg ttcaacaccg
     2821 tttaacacct cgatctttaa tccctgttcc aacaggtact gtaagccggc cgtcacattg
     2881 gcgtccgtcc cgactacctc gaccaccagc gatccaaagg ggttatcctt gatgaaatca
     2941 agattggcgt atatgatatt cacgtcgaca ttaaactgcc ggatgagaga ggtgatcacc
     3001 ggttccccgg ctaccgatcc gatgaacgaa atatggacgg tcttgccgat ctgtccagcg
     3061 cgatgcaaaa tgatcttgcg ttgtttgatc tcttcgggaa tctccgtatg cagaacactc
     3121 ttgataaaac cccgggccgt attggtgcgg ggatgagtaa agagctccac taccggacct
     3181 gcttcgataa tccggccgtc ttcgatgaca gccacgttat cacagatttc tttgatgaca
     3241 ttaatctcat gagtaatcaa gacaatcgtc agttccagac ggcgattgag atcctgcaac
     3301 aatttcagga tggatttggt ggtctccggg tccagcgccg aggtggcctc atcacaaagc
     3361 agcaccttgg gacggttggc caaggcgcgg gcgattccca cccgttgttt ctggccgccg
     3421 cttaattgag agggataggc gttcaattta tccgcaaggc cgaccaattc gacaagctcc
     3481 ttgacccgtt cacggatttc gtccttaggc attcccgcaa tctccagcgg aaaggccaca
     3541 ttcccgaaga cagtccgggt ggcaagcaga ttaaaatgct gaaagatcat gccgatctcg
     3601 cggcgggctt cccgcaactc tttaccgttt aatgcggtca tctcccggtt tgcgatctcg
     3661 atggtcccgc tttgcggcac ttccagccgg ttgatgcaac ggatcagcgt actttttccg
     3721 gcgccgctca atccgataat cccgaagatc tcaccttttt gaatctcaag gcttacatca
     3781 tttaaggcaa caacccggtt atcccggtct tcatagattt tggtgatatg attcagccgg
     3841 atcatgctct cggtcacctg aaggaattcc tcctttgata gagcgtaaat aatgaaaaat
     3901 aacggaagac gatttcctgg aaaaaaggtt tatttggaaa aaagaatata aaaaatcaat
     3961 cattattaga aagcaaggtt caattgggaa aaaccgtttc cctgggaaaa gattaagtat
     4021 tcctatgaaa atagtgggat aattaatgat attttactgg tttggcatta ttgtgtcaag
     4081 ccatttcaaa gtggcaccgg aattttaatg cggttgtaac ctacatctat ccatcggtat
     4141 tgaaggttga ttcctatata agaagattga attccaccgg gaatttttcg agcagattaa
     4201 tattcattga caaacaatat tgctctatgt ataatattga tcgaaggtga ggatatggat
     4261 ccacaaatca aaaaaggaat cttggatgtc atcgtgctat ccctgctccg caaggaggat
     4321 acatacggtt acctgttatc ggtacaggtg gcgcagatgg tcgcgattgc ggagaccgcc
     4381 ctttacccgg tattgcggcg gcttgaggcc caggggttgc ttgagacgta ttcggttgag
     4441 tacggaggcc ggttacggaa atattaccgg attaccgccg ctggaatcac ccggctcaac
     4501 cagtccctgg atgagctttt ggaactgaag gaactcattg acaagattat ggaaggagtc
     4561 gaggaccatg gcggaacgaa ccgctgagtt tatacaatcg gtccgggaag ggctggcgga
     4621 tctcccggcg gatctgaccc agggggcttt ggattattac gaggagttta tcgcggaggc
     4681 tttggaatcg ggtatctccg aaaccgaggt gttgaaaaga ttgggacggg ccaccgatgt
     4741 agtagctgcc atcagggcgg agcggtttat cgatcgggcg gaacaacgcc ccggaccgct
     4801 gggattgatt caagcctccg gtaaagcctt tgggggttat gctttttcgg ccggcagact
     4861 tctactggca atcggaccgc tctgtatggt cttaatcggc ggaatgttct atataacggc
     4921 ggtgttatct tggatcggcg cacttgcgag cattccggta attctttcct caatcactca
     4981 gatcgtgccg gagtattgga ccggaaagct cagtttagtc ggaacgggat tcattgcttt
     5041 ttccctacta gccggaacgg gggtcgggtt ttggcaggca gcggactgga tgaccaaact
     5101 caccatgcgc tggttgcgcc gcctgattca gccgcgtccc aaccggaaac cgcaacccgt
     5161 caggaatgag tcggaaaaga tgaaaccggg aatgaagatt ctgcttcggg tatgttgcgg
     5221 tctgttctta gccggagtcc cgctgttatt ctcatcggat ttacctgtta actgcttcac
     5281 tatctggaac agctgggttc ccggggattt aacatcttcc acccgggagt tcgttccgga
     5341 ggaaatccgg cgaattacga taaacacttt aaactcgcga atcgagattg agcccgcgga
     5401 tatcgcggtg atcaaattga cttacgggca accggagtgg atggattgca cgctcgaaac
     5461 ccgcggcggg gagttgcaat tcaccgagaa gtcggccggt aagattccct atatgaattt
     5521 tatcgcccgc catgagggga tgactgtgat tcggctggaa gtcccgcggc gctatgccgc
     5581 ggatgctgtg gagctggtga gcatcggcgg tcatatgatc cttgatttga gggcggagag
     5641 catccaggcg agcaccgggg atgggaatat tcgttaccgg tcccgcggtc cgggggaggt
     5701 aatccgtgcc gggacgcagc ggggcagtat ctgggtccgg ggagcggtgg taggaaagtt
     5761 gacgtttgag aggggaaagt cgggacggcc ggtggttttg tggagtgatg gcgggaagat
     5821 tgagatcgaa taagggtgtg tggggatgga gtttaaaaac tttgccggat tggttgaggc
     5881 gccgtgaaaa gctgcgcatt gctgcgtc
//
LOCUS       DASVMB010000196         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_170578,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000196 DASVMB010000000
VERSION     DASVMB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170578"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF531_05990"
     CDS             complement(<1..340)
                     /locus_tag="VF531_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7713552.1"
                     /translation="MKIETTLAHAGLCTDGTTGAISTPIYQTAAFSHPALGASTGYDY
                     SRTANPTRQVLENVMAGLEKGDRGFAFSSGMAAITAVLMLFSPGDHLVVSDDLYGGTY
                     RNFEKNFKPYG"
     regulatory      complement(412..553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(769..1542)
                     /locus_tag="VF531_05995"
     CDS             complement(769..1542)
                     /locus_tag="VF531_05995"
                     /inference="COORDINATES: protein motif:HMM:NF022604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX7713553.1"
                     /translation="MNDTSAYSLPPLDMARENCFWLRIFRDHSQFIMDTLAPGQTSLI
                     AQAQQFYQIFNQLLGRAQESTGAFLQEAGRAATDFRCYQLNIVNLQLAGQNPINLPPG
                     ALNEMLDEIDEYLRLIKVAPGIAPMNEAAALIHQHLLWLPNNAAHAALLRAQLDPGEA
                     TFFEVFNRYFKLFSKMASKVMELQGLMRDNPRLVPSLLYTTAESAGLTRDFRGVLQNY
                     RMLRASAQVLGISPPLLMDHLEREAAYYLEKIGYASLLG"
     gene            complement(1822..2736)
                     /locus_tag="VF531_06000"
     CDS             complement(1822..2736)
                     /locus_tag="VF531_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7713554.1"
                     /translation="MVIGGIDAGGSATKCILIDETRKVLGESRSGSANYQAGAAKAAA
                     EIRVAIKSACELAGVDHIDALGIGVAGAGRDADLEAMRREIGPMEMVESSWLTNDGAI
                     AVLGAHGGKPGVVLIAGTGSIAYGLRQDLSMVRSGGWGSILGDEGSGYWIGLQALKAF
                     ARSLEGRDEPTVLTQLIRNRFGITDPGELLPVVFKPDLGRKEVAALAPEVIEAMIRGD
                     RMALRIIHTGLGELVLLVQSVRKNLGFTPKTVAVSGGLFQNHFFYEQFAAQLKAATDF
                     SATEPLFPAVYGAAFFGAARAGIMFPED"
     gene            complement(2753..>3494)
                     /locus_tag="VF531_06005"
     CDS             complement(2753..>3494)
                     /locus_tag="VF531_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713555.1"
                     /translation="LLSAFKTTQELFFNPFGLPKQFSFEIFARVWNRAHFNIYFANSV
                     MISVASLILLLVTASLAAYGLGRFKFRMNNLIFIFFLAGIMIPMRLGILPLFILMKNL
                     SLLDKSLSLILTYAASGLPMSVFILTGFFKNIPFELSDAARVDGCSELQILNRIMLPL
                     TRPALATVTIVNFVPWWNDFFFPLLFIKSDQLKTIPLGMTVFFGEYMTDWSMLFAGMV
                     MASIPLLILYLLMSKQFISGLTAGAVKG"
BASE COUNT          918 a          947 c          847 g          782 t
ORIGIN      
        1 ttccgtacgg tttaaaattt ttttcaaagt tccggtaagt ccctccgtat aaatcatcgg
       61 aaacgaccaa atgatcaccg ggagagaata acatcagcac cgcagtgatc gccgccattc
      121 cggaagagaa agcaaatccg cggtcacctt tttccaaacc ggccatgacg ttttctaaaa
      181 cctgacgggt cggattggct gtccgtgaat agtcatatcc ggttgaagca cctaaagccg
      241 ggtgactaaa agcggccgtc tgatagatgg gggtgctgat cgcgccggtc gtcccgtccg
      301 tacacaaacc agcatgagcg agcgttgttt caattttcat gagaaccctc ctgtatatca
      361 aataaaaaag ccccttcccg gaggaagagg ttgcacatag ctaccatcta cctctcatct
      421 ctcaggtttc cctgcaggaa ttggcacctg ttcattgaag cgcagtctca aagaaactga
      481 taaattcggc tacgccgaaa attaggttgc cgagggttca ttgggccagt ccctccccct
      541 ctccggataa gagcaattta tttaattaga ttaattataa gtaagatcat gggtttagtc
      601 aaatattttt ttatttaact cctcccgccc cccgggagca tatgcgtccc ggtccagatc
      661 gaaggagcgg aacgatgaaa agtcaagtga ggtcataaac aaaaagactg tctcaaggac
      721 agccttagaa gtttaatctc taatcaatca tcgatcaacg ttttttcatt acccaaggag
      781 cgatgcatag ccgatctttt ccaggtaata agcggcctcc cgttccaagt gatccattag
      841 caacggcggg gagatcccta aaacttgggc tgaagcccgt agcatccggt aattttgcag
      901 taccccccgg aaatcccggg tcaaaccggc gctttcagcc gtggtataga gtaatgaagg
      961 caccaaacgc ggattatccc gcattaaacc ttgcagctcc atgaccttgc ttgccatttt
     1021 ggaaaagagt ttgaagtacc ggttaaacac ctcaaagaaa gtggcctctc cgggatctag
     1081 ttgtgcccgg agtaatgcgg catgagcggc attattgggt aaccataata aatgctgatg
     1141 aatcagggca gccgcctcgt tcatcggcgc aatgcccgga gcaaccttga tcaaccgcaa
     1201 atattcgtcg atctcatcca gcatctcatt caatgccccg gggggcaggt tgatcgggtt
     1261 ttgcccggcc agttgcaggt tgacgatgtt cagctgataa caacggaaat ccgtcgccgc
     1321 ccggccggct tcttgaagaa aagccccggt ggactcttga gcccgcccca acagctgatt
     1381 aaaaatttgg tagaattgtt gcgcttgggc aatgagactg gtttgaccgg gcgccagggt
     1441 atccatgata aattggctgt gatcgcggaa gatccttagc cagaaacagt tctcacgcgc
     1501 catatccagc ggcggcaatg aatacgcgga cgtatcgttc atctttcaac ccccgttccg
     1561 aaataactgt atgtaaccgt tccatgcggt gcacagacat attcagcttc atgcatattt
     1621 agcttattcc gtgccggggg atgatgtgtg acaacggcgg gtaaatttga ggaacagcgt
     1681 caaccggttc acattgagaa agggaggagg ctgtacacca tggccaaaaa aatatcccct
     1741 ccggggtgtc gaaggggtac tgtggatgta tccatcctct atccaatcaa gcaggccttg
     1801 ttccgtagcg atattccgga gtcaatcctc cggaaacata atcccggcac gggccgctcc
     1861 aaaaaaagcc gctccgtaca cggcaggaaa caaaggttca gttgcggaga aatcggtcgc
     1921 cgctttaagt tgggctgcaa actgttcata aaagaaatgg ttttgaaaaa ggccgccgct
     1981 gaccgccaca gttttcgggg taaaacccag gttcttccgg accgactgta ccaaaagaac
     2041 caactccccg agcccggtat ggatgatgcg caatgccatc cggtctcccc ggatcatggc
     2101 ctcgataacc tccggggcca gagccgccac ttccttcctg cccagatccg gcttgaagac
     2161 gacgggcaac aattctccgg ggtccgtgat cccaaaccgg ttcctgatga gctgggttaa
     2221 aaccgttggt tcatcccggc cttccagaga cctggcgaac gctttcagtg cttgtagccc
     2281 aatccaatag ccgctgccct catcgccaag aatggaaccc cagccgccac tgcgaaccat
     2341 gcttaagtct tgccgaagtc cgtaagcgat ggaaccggtt ccggcgatca gtaccactcc
     2401 cggtttacct ccatgggcac ctaatacagc gatcgctccg tcgttggtga gccaagagct
     2461 ctccaccatc tccatgggcc caatctcccg gcgcatcgcc tccaaatccg catcccggcc
     2521 cgctccggcc acaccgatcc ccaaagcgtc gatgtgatcc acacccgcca gttcgcaagc
     2581 ggattttatc gccacgcgga tctccgcagc cgccttggcc gcacccgcct gatagttggc
     2641 ggatccgctt cgggattcgc ccagaacctt acgggtttca tcgatgagaa tgcatttcgt
     2701 ggccgaaccg ccagcatcaa ttcctccgat taccatattt ctccccctcg gatcaacctt
     2761 tcaccgcacc ggccgtcaag ccgctgataa actgtttcga catcaacaga tataggatga
     2821 gcaacgggat gctggccatg accattccgg caaagagcat tgaccaatcg gtcatgtatt
     2881 cgccgaaaaa taccgtcatc cctagcggga tggtcttcaa ttgatcggac ttgataaaca
     2941 gcaaaggaaa gaagaaatca ttccaccatg gtacaaagtt gactatcgtc acggtggcca
     3001 gggccggccg ggtcaaaggc agcataatcc ggtttaaaat ctgcagttcg ctgcagccgt
     3061 caacccgggc ggcgtcactc aattcaaagg ggatattttt gaaaaaacca gtcaggatga
     3121 aaaccgacat cggtaaacca ctggctgcgt aagttaggat cagcgatagg ctcttatcaa
     3181 gcaaagataa gtttttcatc aatataaaga gcggcaagat tcccaaccgc atcgggatca
     3241 tgattccagc caggaaaaat atgaagatca ggttattcat ccggaatttg aaccttccca
     3301 gaccgtatgc ggcgagagaa gcggtcacca acagaagtat cagggaggcc acggatatca
     3361 tgacgctatt tgcaaaataa atattgaaat gggccctgtt ccaaacccgg gcgaagatct
     3421 caaaactaaa ttgcttggga agaccgaacg ggttgaaaaa aagttcctga gtagtcttga
     3481 aggcgcttaa cagc
//
LOCUS       DASVMB010000197         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_614377,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000197 DASVMB010000000
VERSION     DASVMB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614377"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF531_06010"
     CDS             complement(<1..741)
                     /locus_tag="VF531_06010"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX7713556.1"
                     /translation="MMVKKIGVLTGGGDCPGLNAVLRAVVKTACGRCGCDVIGFRDGY
                     HGLVLNDYVRLDPTNVSGILDRGGTILGSSNRDNPFNFRIEENGVARFADMSGQVVAN
                     LKMLGIEALVLIGGDGTLTSARDFARMGINVIGVPKTIDNDLSATDLTFGFMTAVDTA
                     TTAIDKLHSTAEAHHRIMILEVMGRYAGWIALESGLAGGADMILIPEIPFDLNKVAQK
                     AIERKNSGKNFSIVVVAEGAKPKGGEMVI"
     gene            946..>2770
                     /locus_tag="VF531_06015"
     CDS             946..>2770
                     /locus_tag="VF531_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7713557.1"
                     /translation="MAWIADLHIHSHYSMATSKDCTPRNLYRWAGLKGVALVGTGDFT
                     HPGWRKELSAELLPAEPGFYTLKEPPLRELPDAPAARFVVSGELSSIYKKNGKVRKVH
                     NLIILPSLEAADRIAVKLEELGLNIRSDGRPIIGMDSRDLLELVLNLCPEAIFIPAHI
                     WTPHFSVFGSNSGFDSIEDCFEDLTSQIYALETGLSSDPAMIWRWSALDRFTLVSNSD
                     AHNPQNLAREANLFTAEFSYDGMREALRNKAAGNFAGTLEFFPEEGKYHYDGHRNCGI
                     AWHPEETLAQAGICPVCGKKVTIGVLHRVIALADRETGFQPKDAAVYQSLVPLREVIG
                     SAFGKGAGSRKVEQCYFDLLHKFGPELTVLRDAPLEEIIRIGGPLVSEGLRRLRAGKV
                     GTQPGYDGEYGAVSVLNESDRLALQGQAALFETAAAGGEVRNTRWETQLQKAVAGIVD
                     EARSMVTEAAIPEQKSDSSGEQRRIIHSDAACLIVIAGPGTGKTRTLVERIAYLVREA
                     GAGPEEITGVTFTNKAAVEIRNRLQNVLAGDKRVQRLNLGTFHGLAWRILNRNPEYAH
                     IKLLEETETREIIEDVLRKERIPMTAREASLIISLMKNKYLW"
BASE COUNT          649 a          693 c          751 g          677 t
ORIGIN      
        1 aatcaccatt tcgccgcctt tgggtttcgc cccttcggca actaccacaa tgctgaagtt
       61 tttcccggaa ttctttcgtt cgatggcctt ttgagccact ttatttagat caaaggggat
      121 ctcggggatc agaatcatat ccgccccacc tgcaaggccg gattcgaggg caatccaacc
      181 tgcgtagcgg cccatcactt caaggatcat gattcggtga tgcgcttcgg ccgtactatg
      241 tagcttgtct atggccgtgg tagctgtatc caccgcggtc atgaatccga aggtgagatc
      301 ggtggcggag agatcattgt caatggtctt gggaacaccg atcacattaa tgcccatccg
      361 ggcgaaatcc cgggcgctgg ttaaagtgcc gtcgccgcca attagtacta aagcctcgat
      421 accgagcatt tttaaattgg ctaccacttg acccgacata tcagcgaaac gagcgacacc
      481 gttttcttca atccggaaat tgaaggggtt gtcccggttg gatgaaccca ggatggtacc
      541 gccgcggtca aggatacccg agacgtttgt tgggtcgagc cggacataat cattgaggac
      601 cagtccatgg tagccgtcgc gaaaacctat tacatcacaa ccgcacctgc cacaggctgt
      661 tttcaccacc gcccgcagta ccgcgtttaa accgggacag tcaccaccac cggttaagac
      721 gccaatcttt tttaccataa ttccacctct ttctcataat cggaagaacc cacggtgtct
      781 ctgagtcttg tgcattcccc tctagtatta gcgccttgga ctggaaaccc tccttttttc
      841 gatcatttca tgaagatttc atcatttttt tgcagggttc atttcgtgaa tgtggaaaat
      901 aaaagcggtt aaatataggc ttaaggatat ttaggagtgg tctttttggc ttggattgcc
      961 gacttacata ttcattccca ctattccatg gcgaccagca aggattgcac cccccggaat
     1021 ctataccgtt gggcggggtt gaaaggagtc gctctggtgg gaaccggaga ttttacccat
     1081 cccggctggc gcaaggagtt atccgctgag ttactccccg ccgagccggg tttttataca
     1141 ttgaaggagc cgccgctccg ggaactccct gatgcacccg cggcacgttt tgtggttagt
     1201 ggtgaattaa gctcgatcta taaaaagaac ggtaaggttc gtaaagttca taacctaatc
     1261 atattgcctt ccctggaagc agccgatcgg atagccgtca aattggaaga attggggttg
     1321 aatatccgct ccgacggccg gcccatcatt ggtatggaca gccgtgacct tttggaactg
     1381 gttttgaatc tttgcccgga ggccattttc ataccggctc acatctggac acctcatttt
     1441 tcggtatttg gttccaactc cgggttcgac agtattgaag attgtttcga ggatttgaca
     1501 tctcaaatat acgccctgga aacgggatta tcctccgatc ccgcaatgat ctggcgctgg
     1561 tcggcgttgg atcggttcac gttggtctcc aattccgatg cccataatcc gcaaaatctt
     1621 gcgcgggagg ctaacctgtt tacagcagag ttttcatatg acggcatgcg cgaagcctta
     1681 cggaataaag cggccggtaa tttcgccggc actctcgaat tctttccgga ggaaggcaag
     1741 tatcattatg acggtcatcg gaattgcggg atcgcttggc acccggaaga gaccttggcc
     1801 caagccggaa tctgtccggt ttgcgggaaa aaagtgacaa tcggtgtctt gcaccgggtc
     1861 atcgcccttg ccgaccggga aaccgggttt caaccgaagg atgcagcggt ttatcaaagt
     1921 ttggtgccgc tcagggaggt aattggctcg gccttcggca agggagccgg ttcccgtaag
     1981 gttgaacaat gttattttga tttgcttcat aaattcggac cggaattgac ggtgttgcgg
     2041 gatgcaccgc tcgaagagat catccggatt ggagggccgc tagtatccga aggtttacgt
     2101 cgactacgcg cggggaaagt cgggacccag cccggatacg acggtgaata cggcgcggtt
     2161 tcagtcttaa atgaatcgga tcgcttggcg ttacagggtc aggcggcact tttcgagaca
     2221 gcggcagcag gtggagaagt acgaaatacc cgatgggaaa ctcaattaca gaaagcggtg
     2281 gctggaatcg tcgacgaggc ccgcagtatg gtcaccgaag ccgccatacc ggagcaaaag
     2341 tcagattcta gcggggagca gcgccgaatc atccattccg atgcggcctg cctgattgta
     2401 attgccgggc cgggaacggg taaaacccgg actttggtgg aacgcatcgc gtatttggtt
     2461 cgggaggccg gggcgggtcc ggaggagatc accggagtaa cctttaccaa taaagccgct
     2521 gtcgagatcc ggaaccgttt gcagaatgta ttggctggag ataaacgggt gcagcggtta
     2581 aatcttggaa cctttcacgg attggcctgg agaatcctaa accgcaatcc ggagtatgct
     2641 cacattaagt tgctggaaga aaccgaaacc cgcgagatca tcgaggacgt gctccggaag
     2701 gagcggattc ccatgactgc ccgggaagct tcactcatta tctcgctgat gaagaataaa
     2761 tatctttggg
//
LOCUS       DASVMB010000198         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_318759,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000198 DASVMB010000000
VERSION     DASVMB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318759"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF531_06020"
     CDS             complement(<1..185)
                     /locus_tag="VF531_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7713558.1"
                     /translation="MKSLDYDLIVVGAGHAGCEAALAAARLGAATLIITITPDTIAAM
                     SCNPAIGGPAAKSHLVR"
     gene            complement(209..1591)
                     /gene="mnmE"
                     /locus_tag="VF531_06025"
     CDS             complement(209..1591)
                     /gene="mnmE"
                     /locus_tag="VF531_06025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7713559.1"
                     /translation="MYLNDTIAAIATPVGEGGIGIIRISGPEVKKIARALLVRPSRCS
                     YPELEDHRVYYGLAVDPETGATLDEVLFFYTQKPRSYTAEDTVEIQAHGGTLVLSKIL
                     AAVLRQGARMAEPGEFTLRAYLNGRIDLVQAESVIDLIRAKTDKAHQLALSQLSGTAT
                     RALQELETGLYQILIAIEAVLDFPEEGLPEPTQHVMLEQTGRLHQQLTAMLAEAATGR
                     KIREGITVVIAGRPNVGKSSLLNKLLQEERAIVTEIPGTTRDLIEAQIQLYGVPVRLV
                     DTAGLRPTDNPIEQIGIQKAEAEMAEADLILLVLDSSQPMNEVDREIIDKVRGKKVLL
                     IFNKIDLPRRLEPQPLSEKNDFKILEISVASGEGFERVEQAIVEMVGLGELRVDDRPL
                     LSRVRHKDALERSLTALDSFRTGLARGVSEDLLAVDLRSCLGALGEITGKNVTDEVVH
                     GIFAQFCIGK"
     gene            complement(1847..2482)
                     /gene="jag"
                     /locus_tag="VF531_06030"
     CDS             complement(1847..2482)
                     /gene="jag"
                     /locus_tag="VF531_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX7713560.1"
                     /translation="MKFVEKIGRSQEEAVAEALQELGVGPDQVRVEVLEELTNKGLFG
                     LLGGGKTVKVRVTVKEDLGQAAGLFLREMLVNMGILAQVEVFRRKDNTTLNINGKDLG
                     ILIGKHGQTLDAIQYLVNLSINKDQPEKERIIVDVEGYRHRREETLKKLALRMADKVK
                     RENRKQVLEPMSPHERRIIHATLQNYKEVFTYSEGEDPYRHVIISPQDNQT"
     gene            complement(2509..3198)
                     /locus_tag="VF531_06035"
     CDS             complement(2509..3198)
                     /locus_tag="VF531_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX7713561.1"
                     /translation="MNIIKPFVDLFRIVLQFFFENTQSWGWAIILLTLLIKIVLFPMN
                     VKQFQAMEKMKRIQPKMKEIQDKYKDKPEELQRRTMELYQKEKVNPFGSCLPLLIQLP
                     LLWAIFALLQDPKNGIASVIASSPFFWFTLGAKGDILLAILSGVTTFFQQKLTTPTAG
                     AVDSSQKTMLYIMPLMFAFFTWTVTNGIGLYWVASNIIGIAQQYLINEYFIVKEHIKK
                     DNEPTENPTKK"
     gene            complement(3239..3463)
                     /gene="yidD"
                     /locus_tag="VF531_06040"
     CDS             complement(3239..3463)
                     /gene="yidD"
                     /locus_tag="VF531_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7713562.1"
                     /translation="MRGVILLFITVYQKFISPLKPRCCRYYPTCSAYAYQAVQRFGVI
                     KGSWYAMKRLLRCNPFSDGGYDPVPEVKES"
BASE COUNT          871 a          943 c          792 g          952 t
ORIGIN      
        1 tcccttacca ggtggctctt ggctgccgga ccgccgatcg ccgggttaca ggacatagcg
       61 gcgatagtat ccggagtaat tgttataatt aaggttgcgg cacctaaccg ggctgcggca
      121 agagccgcct cacacccggc atgaccggcg ccaaccacaa tcagatcata atccaagctt
      181 ttcaatcctt tctaatgact caaccttttt atttgccaat gcaaaactgg gcgaaaatcc
      241 cgtgaaccac ctcatcggtg acgttttttc cggtaatctc gcctagggca cccagacaag
      301 accggaggtc aacagccaat aaatcttcac tgacccctct agccaagccg gttcgaaaac
      361 tatccaacgc tgtcagactg cgttccaggg cgtctttatg gcgaaccctg gataataacg
      421 gccggtcgtc cacacggagt tcgcccaatc caaccatttc tacaatggcc tgttccactc
      481 tttcaaagcc ttccccggag gccaccgaaa tctccagtat tttaaagtcg ttcttttcgc
      541 ttaagggctg cggctccaat cggcggggaa gatcgatctt gttgaatatc aagagaacct
      601 ttttaccccg cactttgtct atgatttccc gatctacttc attcattggc tgactgctgt
      661 ccaaaaccaa taggatcagg tccgcctccg ccatctcagc ctcagctttt tggatgccga
      721 tttgttcaat tggattgtcg gttggtcgta atcccgcagt atcgaccaac cttaccggga
      781 ccccgtataa ctgaatctgg gcttcgatca gatcccgggt ggttccggga atttctgtca
      841 cgatggcccg ttcctcctga agcaacttgt ttaaaagact tgacttgccg acattcggcc
      901 gaccggcaat aaccaccgtg atcccttccc ggattttccg ccctgtagcg gcttcggcca
      961 gcatcgccgt taattgctga tgcagccgcc cggtctgttc tagcatgaca tgttgagtcg
     1021 gttccggcag cccctcttcg ggaaagtcca ggaccgcctc gatagcgatt aagatctgat
     1081 ataagccggt ctccaattcc tgtaacgccc gggtggcggt cccgctaagc tggctcaggg
     1141 ctaattgatg ggctttatcg gttttggccc gaatcaggtc aattaccgat tccgcctgta
     1201 ccagatcgat ccttccgttc aaataggcac gtaaagtaaa ttctcccggt tcggccatcc
     1261 gggcgccctg ccgtaatacc gctgcgagaa tcttggacaa caccaatgtc ccaccgtggg
     1321 cttggatctc caccgtatct tcagcggtat agcttcgcgg tttttgagta taaaaaaaaa
     1381 gcacttcgtc cagggttgca ccggtctccg gatccacagc caaaccgtaa taaacccgat
     1441 ggtcttccag ttccgggtaa gaacaccggc taggccggac aagtaaagcc cgggcgatct
     1501 ttttaacttc cgggccgctg atccggatga ttccgattcc gccctcccca accggggtgg
     1561 cgatggcggc gatagtgtcg ttcaagtaca tagaaagcct gcttcccttg gatgtgaatt
     1621 tgcctaaacc acttctggta ctcgtttgca tcaaccattg aacaaaacag gcacgcgcgc
     1681 gatgcaaatt ggtccgaaaa attaggtttc ttttcaatgt ttcaatagaa acacttagta
     1741 ttagtttaac ataactcggg gtttcttaaa agccgggttt ctcccgatga agcacaatga
     1801 agtcgaagcg ctacgaagtc gaaaaaaaaa cccggcgatg ccgggttcag gtctgattat
     1861 cctgtggtga gatgatcacg tgacggtatg gatcttctcc ctcgctataa gtgaagactt
     1921 ctttataatt ttgtaaagta gcgtggatga tccgtcgttc atgaggagac atcggttcca
     1981 gtacctgctt cctgttctcc cgtttcacct tatcggccat acgcagagcc aattttttca
     2041 aagtttcttc acggcggtga cggtaacctt ccacatcgac gatgatccgt tccttctccg
     2101 gctggtcctt attaatggac aaattaacca aatactgaat ggcatcaagg gtttggccgt
     2161 gtttaccgat gagtatcccc agatccttcc cgttaatgtt caaggtggtg ttgtccttgc
     2221 ggcggaagac ctcaacctga gccaaaatac ccatatttac cagcatttca cgtaggaata
     2281 agccggcggc ttgtcccaga tcctccttga ccgtaactcg aaccttcact gtcttgccgc
     2341 cacctaaaag gccaaagagg cctttattgg taagctcttc taaaacctcc acccgtacct
     2401 ggtcgggacc aacccccagc tcttgcaggg cctcagctac agcttcttct tgggaacgac
     2461 cgattttttc gacaaatttc attataccag tcctccttta tagctggcct acttcttggt
     2521 gggattttct gtaggctcgt tatccttttt aatgtgttcc ttcacgatga agtactcgtt
     2581 aatcaaatat tgctgcgcaa tgccgatgat gttggaagcc acccaataaa gaccgatacc
     2641 attcgtgacc gtccaggtga aaaaggcaaa catcaaaggc atgatgtaca acatcgtctt
     2701 ttgactggaa tcaacagctc cggctgtggg agtggtcaat ttttgctgaa agaaagtggt
     2761 gactcccgat agaatcgcaa gtaaaatatc gcctttggca cccagtgtga accagaaaaa
     2821 aggcgagctc gcgatgacac tcgcgattcc gtttttagga tcttgtaaca gggcgaagat
     2881 tgcccagagt aaggggagtt gaatcaaaag aggcaaacag cttccgaagg ggttaacctt
     2941 ctccttctga tataactcca tggtccggcg ctgtaattct tccggcttat ctttgtattt
     3001 gtcttgaatc tccttcatct tcggctggat ccgcttcatt ttttccatgg cctgaaactg
     3061 cttgacattc atcggaaaca gcacgatctt aattaaaagg gtcaataaaa taatagccca
     3121 accccagctt tgggtatttt caaagaaaaa ctggaggacg atccggaaca aatcaacaaa
     3181 aggctttata atattcaagg tcaacatcct ttcccggttc aatcttaccg tatctcattc
     3241 atgactcctt tacctcgggt accggatcat aacctccgtc actaaagggg ttacagcgta
     3301 ataatctttt catcgcgtac caggatccct ttattacgcc gaatctttgc acggcctgat
     3361 aggcataggc cgaacaagta ggataatacc ggcagcaacg cggttttaaa ggtgaaataa
     3421 atttctgata aacggttata aaaagaagga ttactcctcg catcgggaat cctccgtttc
     3481 tcccttattt ggcttggtta agagcttcgc tttcacaagt atatgttcaa tttctatgga
     3541 tataactttc agatcggc
//
LOCUS       DASVMB010000199         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_170993,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000199 DASVMB010000000
VERSION     DASVMB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170993"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VF531_06045"
     CDS             complement(<1..726)
                     /locus_tag="VF531_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713563.1"
                     /translation="MVTLRRWFIVLLIIMMITLSASAFAAKTEITYMAWYNTTESEAA
                     DIQKTLDKFNSSQNDIHVTLIAVSWNDYNTKLNTMAAAKQLPDCCMMNEAMTIRYAAS
                     GILADVSDMYSASEAPLKSLAFTYKGKTVAYSSANEVLVTYYNKKLFDEAKVAYPPAS
                     ADKAWTWKEFVATAKKLTKDKNGKTPNDPGFDPKNIVTYGADFNRLAWMWPILAVSNG
                     GGIMSSDGQKLLLGRPETIEALQA"
     gene            1074..2072
                     /locus_tag="VF531_06050"
     CDS             1074..2072
                     /locus_tag="VF531_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7713564.1"
                     /translation="MRDVAEKAGVSVATVSAVINQSCKVSERLTSKVLDAAKALNYRP
                     NRLARALSKKQTHLIGCLVPTIVNPFFPQIVKSVEDIAYQNNFGVFVCNSEGNTGKVS
                     YYQQMLIETQVDGVIIALSWELARPEVVNPFLEAGIPVVGMAGARRLEIIDCVVVDDV
                     QGGFEAANHLLSSGHTKIGFIGAQNSETTRLRLLGYQKALETAGLNYEERYFVLGSSF
                     SEVEAYTLTKVLLGRCPEITAILAYNDMMALGVISALADIGLTVPADISVMGFDDSVA
                     SLSIPKISTIAIPKDEMGRIAANILMQRIGGGTGSPQIYEIRPQLVVRNTTARVHD"
     gene            complement(2156..3784)
                     /locus_tag="VF531_06055"
     CDS             complement(2156..3784)
                     /locus_tag="VF531_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713565.1"
                     /translation="MMRFLIIDDDVFVHTNLKKMINWEAEGFSLYDAAIYGAEAVRMI
                     DQIGPDIVITDMNMPGIDGVGIIRYLLEKHTSIKVIALSAYDDFEYVKQSLKLGAVDY
                     ILKHSLTPEVLLTLLHGVRETIFREKRKGEEEQRLDEQIQTGRSFLQQNFIKTLIRDG
                     TRDHAMTVRRIHDLGLRLSSKNLVVVAGEIDDYSALPEKFSVSEIENLMKSFINMGTE
                     ILKDTVPSLITILEEGKFVMIFSFEAIRSNQEIYHQVFTTLTRIKTTIKRYLNLTACF
                     GLDGICPDLTELDRYYQKARRQLDKKFYAGKDMIFHDPLTVEMKNDLHILEIKDEKRI
                     FDMIQSGKSEELRLYLDKIFERIKQDRPSPNSTKIAFVTLINIANKVARDTVIDLALI
                     YGDGCNPYEHMERLETAEEVKAWLVQVYEKAINLLELFHLNPDYDELIKKTIGYIHKN
                     YKTGISLCDIAESIGVNSSYLSRKFKQDCGKGIAEYLNDIRIEQAKLLMKNGLKKVKE
                     IACEVGFNNYNYFFKVFKECEGMTPLEYEKSCRG"
     gene            complement(3762..3932)
                     /locus_tag="VF531_06060"
     CDS             complement(3762..3932)
                     /locus_tag="VF531_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713566.1"
                     /translation="MPGYAAPQKTTLTFWTFQELHKGFWDDAVATWNKAHPDEQIVLK
                     TDVYNYDEISHH"
     gene            complement(4037..4336)
                     /locus_tag="VF531_06065"
     CDS             complement(4037..4336)
                     /locus_tag="VF531_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713567.1"
                     /translation="MTKDGKDPRLDMDPDGEPEVTMDQETGKIRARGFFKNMVSNHDA
                     LNPGLERGQMEGSHPVDVNAQTDINLGALADPGGMDPAMDRSFLEGMRESVKKRK"
BASE COUNT         1146 a         1142 c         1053 g         1327 t
ORIGIN      
        1 ggcttggagc gcttcaatgg tttcgggtct tcctaataac agtttttgac cgtcggaact
       61 catgattccg ccgccatttg aaaccgcgag gataggccac atccaggcca gccggttaaa
      121 atcagcgccg taggttacga tatttttcgg gtcaaagccg ggatcgttcg gggttttgcc
      181 gttcttatcc tttgtcagtt tcttggccgt cgccacaaat tctttccaag tccacgcctt
      241 atcggcgctg gccggtgggt aggcgacctt ggcctcatca aacaatttct tattgtaata
      301 ggtaaccaaa acttcattag cactgctgta tgccacggtt ttgcccttat aggtgaacgc
      361 cagggactta agcggcgctt cgctggccga gtacatgtcg cttacgtcgg ccaggattcc
      421 tgaagccgca taacggatgg tcatggcttc attcatcata caacaatcgg gaagctgttt
      481 tgcagcggcc atcgtattaa gtttggtatt gtaatcattc cagctgactg cgatgagcgt
      541 aacgtgaata tcgttctgag atgagttaaa tttgtccagt gttttttgaa tatccgccgc
      601 ttcggactcg gtggtattat accaagccat ataggtgatc tcagttttgg cagcaaatgc
      661 agaggctgaa agggtgatca tcatgatgat taacagcaca atgaaccaac gtcttaaagt
      721 gaccattaaa aatcttcctc ctttaatgat actgtatatg ccgtcttggg caacttcaat
      781 cctttcgggt ccttcacctc cttcacttat tttcatccgt ggaaccgctt gaccgcttca
      841 ttcgtttgag agaacggata cttccgactt gagatttaga gaagtcggtg tcctgaagcg
      901 gcacttaaat ctaaccggtt agattttatt aaataattca tcccgtcttg gaaaaatcct
      961 cctttatgat agaatatttt aacgaataat ttaatcatca tggcaggatt tatattatca
     1021 acaacgaatt aatcggttag attcgttaag ggaggccttt ttaactggct acaattcgag
     1081 atgtcgctga aaaagcgggg gtttccgtgg cgaccgtatc cgcggtaatc aatcaaagct
     1141 gcaaggtaag cgagcggctt acctctaaag tacttgatgc tgcaaaagcg ctaaattatc
     1201 gtcccaaccg gctggcccgg gcgctttcca aaaaacagac ccatctcatc ggctgcctgg
     1261 tcccgaccat tgtgaacccc tttttcccgc agatcgtcaa aagtgttgaa gatatcgcct
     1321 atcagaataa cttcggggtg ttcgtttgta atagtgaggg taacacggga aaagtctcct
     1381 attatcaaca gatgcttatc gagactcagg tggatggggt gattatcgcc ctctcctggg
     1441 aattggcccg gccggaagtg gtcaatccgt ttctggaagc cggaatcccc gttgtcggta
     1501 tggccggcgc gcggcgcctg gagatcatcg actgcgtggt ggttgatgat gttcaaggag
     1561 gatttgaagc agctaaccac cttttatctt cgggccatac caaaatagga tttatcggcg
     1621 cccagaacag cgaaaccacc cggctgcggt tactcggtta tcaaaaggcg cttgaaaccg
     1681 ccggtctaaa ctacgaagaa agatacttcg ttttaggcag ttccttctcc gaggttgagg
     1741 cttataccct caccaaggta ttactgggac gttgcccgga gataaccgcc attttggcgt
     1801 acaacgatat gatggcttta ggggtcatca gcgctttggc cgatatcggc ctcacagtcc
     1861 cggccgatat ctcagtgatg gggtttgatg attcggtggc ttcgttaagc ataccgaaga
     1921 tttcaacaat cgcgattccc aaagacgaaa tgggccggat cgccgccaac attctgatgc
     1981 aaaggatcgg aggcgggacc ggatcgcccc agatctatga gatccggccg caacttgttg
     2041 tacggaatac cactgcccgg gttcatgatt aaccggaact tctcctaatg ttaccttgcg
     2101 gtcattaacc atccccgggg gagacttccc atccatcaat gaagttataa aacgactaac
     2161 ccctgcatga cttctcatat tccaacgggg tcattccttc gcactcctta aagactttga
     2221 agaaataatt ataattattg aaccctacct cgcaagctat ctccttcacc tttttcaagc
     2281 cgtttttcat taaaagtttg gcttgttcaa tacggatatc gtttaaatac tcggcgattc
     2341 ctttaccgca gtcctgtttg aatttgcggc tgagataaga actgttcacc ccgatagact
     2401 ccgcaatatc gcataaggag atgcccgtct tgtaattttt atgaatatat ccgatagttt
     2461 ttttgattag ttcatcatag tcgggattta agtggaataa ctccaataaa ttgatggcct
     2521 tttcatagac ttgaacaagc cacgctttaa cctcttccgc agtctccaaa cgctccatgt
     2581 gctcataggg attgcatcca tcgccataga ttaaggcgag gtctattacc gtatctctgg
     2641 cgactttatt ggcgatgttg atcagggtga caaaagcaat cttcgttgaa ttggggctcg
     2701 gccgatcttg tttgatccgt tcaaaaattt tatcaagata aagccgcagt tcttcacttt
     2761 tcccggactg aatcatgtcg aaaatccgtt tctcatcttt gatctccagg atgtgcaaat
     2821 cattcttcat ttccaccgtt agcggatcat gaaaaatcat gtctttcccg gcataaaact
     2881 tcttatccag ctgccgccgg gccttctgat aatatctatc aagctcggta agatcgggac
     2941 agatcccatc caaaccaaaa caggcggtaa gattcaagta acgtttgatg gtggttttaa
     3001 tccgggtcag tgtggtaaat acttggtgat agatctcttg attgctgcgg atcgcctcaa
     3061 atgagaagat catcacgaat tttccttcct caaggatggt gatgagcgag ggaaccgtat
     3121 cttttaagat ctcagtcccc atatttatga aggatttcat gaggttctcg atttcactta
     3181 ctgaaaactt ctccggtaaa gccgagtagt catcgatctc gcctgcaacc acgactaaat
     3241 tcttggaact cagtctcagc cctaaatcgt ggatccttct tactgtcatt gcatgatccc
     3301 tggttccatc ccggatcaat gttttgatga aattctgctg caaaaacgac ctcccggtct
     3361 ggatctgctc atctaggcgt tgctcttcct cccccttccg tttttcacga aagatggttt
     3421 ccctgacccc gtggagcaga gtcaataaaa cctccggtgt caaggaatgt ttcaaaatat
     3481 agtcgactgc ccccaacttg agactttgct ttacatattc gaaatcgtcg tacgcgctta
     3541 aagcgatcac cttgattgag gtatgctttt ccagcaaata tcggatgatc cccaccccgt
     3601 cgattcccgg catattcata tcggtgatga caatatccgg acctatttgg tcaatcatcc
     3661 gaacggcctc agccccgtag attgcagcgt catataggct gaacccttcc gcttcccagt
     3721 taatcatctt ctttaggttg gtgtgaacga agacatcgtc atcaatgatg agaaatctca
     3781 tcatagttgt acacatcggt ctttaacaca atctgctcat cgggatgggc tttgttccat
     3841 gttgccaccg catcatccca aaaaccttta tgtaattcct gaaaagtcca aaaagtaagg
     3901 gttgtttttt ggggtgcggc atagccgggc aagatcatgc cagttaaaag aagataaact
     3961 tatgagtaga gcccgaattt aacctcttaa aagccataaa aaaaggaggc tggtttcctc
     4021 cccttttagc ctctttttat ttgcgttttt tgaccgattc cctcattcct tcgaggaaac
     4081 tccggtccat ggccggatcc atcccgcccg gatcggccag agcccccagg tttatatcgg
     4141 tctgggcgtt gacatccacc ggatgggacc cttccatctg gccgcgttcc agtccgggat
     4201 tgagcgcgtc gtgattcgaa accatgtttt tgaagaatcc ccgggcccgg atcttgccgg
     4261 tttcctgatc catggtcact tcgggttcgc cgtccggatc catatcgagc cttggatctt
     4321 ttccatcctt tgtcaatttt attacctcca gcgaagttga tggtcttagt atcacccgat
     4381 ccccgtggaa gcaaccgaga atttggaaag aaggcatgag gcaactctcc ccgcggcaac
     4441 cgttactttc cgtatgaccc tactcccctc tcttttcaga aagagcaggg tcgggggtga
     4501 gttgctaata gccaacagag aacaggaaac aggtgtaagg catgaggcaa taggtgtggg
     4561 aaggtatata ttagattgct tcattgcctg agatttcttg cttgcgccgg gctcgaagcc
     4621 tttaccctag cccttaaagg gaattagtac aattctcggg cctttgtt
//
LOCUS       DASVMB010000200         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_171158,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000200 DASVMB010000000
VERSION     DASVMB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171158"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1244
                     /locus_tag="VF531_06070"
     CDS             117..1244
                     /locus_tag="VF531_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HEX7713568.1"
                     /translation="MLKATFPHLGNFYIPFKSFLSELGLEPVAPPLTSQRTVALGTRM
                     SPEFACLPLKITLGNFIEAIDAGAECVFMVGGGGPCRLGYYGEVQREILHENGYGADF
                     IVLEAPQDQPGKMREVIRRYIPRRKPHDFFRAFQIFWRKARAIDCFDLEVNKVRPFAA
                     DPGACTELQTRFYLEVDNASSIAAIQTVYKESVIKLNELSKRDPTFVPLLLLIGEIYM
                     VLEPRVNFQIERTLGSMGVDVRRTIYITDWIKENFLLRYIQPSFQKKRLLTAKPYLKN
                     PVGGHGLESVAHMVESGVNRYDGVIHLAPFTCMPEIVAMQIFPGVSKELSIPVLSLII
                     DEHSAEAGIQTKLEAFTDLLAFRNMQKKKPIESTVPTGLSH"
     gene            1320..1526
                     /locus_tag="VF531_06075"
     CDS             1320..1526
                     /locus_tag="VF531_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1657 domain-containing protein"
                     /protein_id="HEX7713569.1"
                     /translation="MTVASQIKQSLATLQSAHATLRVYAEQSREPESKSVFEDTAKAT
                     GEIIADLEERLKILEFEEPQYKGL"
     gene            1580..2440
                     /locus_tag="VF531_06080"
     CDS             1580..2440
                     /locus_tag="VF531_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEX7713570.1"
                     /translation="MQGWLVILLRSILMFFLTFLGVRLMGKRQPAQMNPFNFTNYVAI
                     AAIAALTGLNIIPNLIWGIIALAVWILFPIALEFLALKSKWVHDLVNGKEAVLIKQGK
                     IMEENLFKVRMTGEELLRELRSKNAFNLSDVEFAVLETTGDLNVLLKSDQKPVTPHDL
                     EWKVAPQAEPQLVILDGNILDEPLASIGQNREWLRLQLEKLGVSLTNVFIGQVNSDGD
                     LFLDLFDDAVQLPQPKVKELLYANLLKCHADMSIFALETHDSEAKAMYNRDSKKLKDL
                     LEKVKPYLLR"
     gene            2457..2936
                     /gene="spoVAC"
                     /locus_tag="VF531_06085"
     CDS             2457..2936
                     /gene="spoVAC"
                     /locus_tag="VF531_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HEX7713571.1"
                     /translation="MSNQKKKKLTPTQQEYQRFAKTREPKRPVWSNVLRAFLVGGIIC
                     TIGQAIQTLFMTYFKFTEVTAGNPTVAVLIIISVLLTGLGVYDHIAQWAGAGTAVPVT
                     GFANSVASAAIEHRSEGYVLGVSAKMFELAGSVIVYGVFSAFLVAIIKLTIKALGGS"
     gene            2936..3937
                     /gene="spoVAD"
                     /locus_tag="VF531_06090"
     CDS             2936..3937
                     /gene="spoVAD"
                     /locus_tag="VF531_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HEX7713572.1"
                     /translation="MIKGHQTWCFESRPVIRATGTVGGPFEAEGAIAGDFDILHGDIW
                     LGESSFEKAERKFLEQACEIAVDKAGMKKADVQFFLCGDLMSQIISSSFAARTLGAPF
                     LGLYGACSSSMEGMAIAASLIDGGHAQNALVATSSHNAASEKQFRYPTEYGAQKPPTA
                     QWTVTGAGAAILTGEGEGPRVTAATMGRVVDMGLSDPFNMGGAMAPAAVDTIEAHFRD
                     LDRDPSYYDLIATGDLGRVGHQIAGDLLEKHGMSLPKDIFTDCGLLIYRKEQSVFSGA
                     SGCACSATVTYGHFLNRMRKGELRKILVVATGALLSPLSYQQKESIPCIAHAVAIEI"
BASE COUNT          995 a          918 c         1061 g          980 t
ORIGIN      
        1 cgggtagtta tgaggacatc gtttctaaaa gtggaaaaga accggcagta tattatcgat
       61 tgggagacgg gaagttataa aagcattctt gaactttaat taaccggaca tttattatgc
      121 tcaaagcgac cttcccccat ctgggcaact tttatattcc ttttaaaagc tttctatcgg
      181 agttaggact ggaaccggtt gcgcccccct tgacttcgca acggacggtt gcgcttggga
      241 cgagaatgtc ccccgaattc gcctgccttc ccttaaaaat cacccttggc aactttattg
      301 aggcgattga tgccggtgcg gaatgtgttt tcatggtcgg cgggggaggt ccctgccggt
      361 tgggatacta tggggaggtt cagcgggaaa tactgcatga aaacggttat ggcgctgatt
      421 ttatcgtact ggaggctccg caggatcaac cagggaaaat gcgggaagtg atacggcgct
      481 atattccccg gcgtaagccg catgattttt ttcgggcgtt tcagattttt tggcggaagg
      541 caagggcgat tgattgcttt gatctggaag tcaataaagt ccggccgttt gcggctgatc
      601 ccggtgcttg caccgaattg caaacccgtt tttacctgga ggtcgataat gcctcgagta
      661 tcgcggcgat ccaaaccgtt tacaaggaat cggtaatcaa attgaacgaa ttgtccaaaa
      721 gggacccaac ctttgttccg ttattgcttc tcatcggtga aatctatatg gtcttagagc
      781 cgcgagttaa ttttcaaatc gaaagaaccc tgggttcaat gggtgtggat gtacgtcgca
      841 ccatctacat taccgattgg attaaagaaa actttctgtt aagatacatc caaccaagct
      901 ttcaaaagaa gcggcttctc acagccaagc cttatttaaa gaatcccgtt ggcggtcacg
      961 ggctggaatc ggtggcacac atggtagaat cgggagttaa ccgttatgac ggcgtaatcc
     1021 acctggctcc gtttacttgc atgccggaga ttgtagcgat gcaaatattt ccgggtgtat
     1081 ccaaggaatt atcaatcccc gtgctttccc tgatcattga tgaacattcc gcggaagcgg
     1141 gaatccaaac caagctggaa gcatttaccg acttgctggc cttccgaaac atgcaaaaga
     1201 aaaaaccaat tgagtctaca gttccaaccg gtttatccca ttaactaatt tatccagttc
     1261 tgaccgggga taaaaaaagg ccattttaag ataatagtag ccgaaaaagg agttttatca
     1321 tgacggttgc ctctcaaata aaacaaagcc tggctacttt gcaaagtgcc cacgccactt
     1381 tacgggtcta tgccgaacag agccgggagc cggagtccaa gtcggtcttt gaagacacag
     1441 ccaaagctac cggagagatc atcgctgatc tcgaggagcg gctaaaaatc ttggaatttg
     1501 aggaacccca atacaaaggg ttataacgcg cgcgcaaatg cggcgtgtgc gtagcacgta
     1561 aacgcgacgg gagaatacga tgcaaggttg gcttgtcatt ttactccgtt ccatactcat
     1621 gttcttcttg accttccttg gagtccggtt gatgggcaaa aggcagccgg cccagatgaa
     1681 ccccttcaac ttcacaaatt atgtggccat tgcggccatc gccgctttaa ccgggttgaa
     1741 tatcattcct aacctaattt ggggaatcat tgcgcttgcc gtgtggattt tattccccat
     1801 cgccttagag ttcctggcgc ttaagagcaa atgggttcat gatctggtaa acggcaagga
     1861 agccgtgctg attaaacaag gcaagattat ggaagagaac ctgttcaagg tcaggatgac
     1921 cggagaggaa ttactccgcg agttgcgttc caaaaatgcc tttaatttaa gtgatgttga
     1981 gtttgcggtt ttggagacta ccggcgatct caacgtgctg ctgaagtcgg atcagaaacc
     2041 ggtaacgccc catgatttgg agtggaaagt tgcgccccag gccgaacccc agctggtgat
     2101 cctggatgga aacatcctgg acgaaccttt ggcaagcatc ggccaaaacc gggaatggtt
     2161 gaggctgcaa ttggagaaac tcggcgtctc gctgaccaat gtcttcatcg gccaagtaaa
     2221 ttccgacggc gatttattct tggacttgtt tgacgatgcc gttcaattac cccagccgaa
     2281 ggtcaaggag ttgctctatg ccaatctgtt gaaatgccac gccgacatga gtatctttgc
     2341 cctggagacg catgattccg aagcaaaggc catgtataac cgggattcaa agaagttgaa
     2401 ggatttgctg gagaaagtca agccttatct cttacgataa ctgcaggtga aaccggatgt
     2461 caaaccagaa gaaaaagaaa ttaaccccga ctcagcaaga atatcagaga ttcgccaaga
     2521 ccagggaacc gaaacgtccg gtatggtcca atgtcctccg ggcatttctg gtgggaggaa
     2581 ttatctgtac tatcggccag gcaatccaga ctctattcat gacttacttc aaatttacgg
     2641 aagtcacggc cggaaaccca actgtggcag tgctgattat tatctcagtc ttattgaccg
     2701 gactgggtgt ctacgatcat atcgcccaat gggccggggc cgggactgcg gtaccggtta
     2761 ccgggtttgc caattccgtg gcctccgcag ccatcgagca ccggagcgag ggatatgtct
     2821 tgggggtcag cgccaaaatg tttgaacttg cgggttcagt catcgtttac ggcgtgtttt
     2881 ccgctttttt agtggcgatt attaaattga cgattaaggc tttgggtggt tcgtaatgat
     2941 aaaaggtcat caaacctggt gttttgaatc ccgtccggtc atccgggcta cgggtacggt
     3001 gggcggccct tttgaggcag agggagcgat tgccggagat tttgacattc ttcacgggga
     3061 catctggttg ggtgaatcca gttttgaaaa ggcggaaaga aaatttctgg aacaagcctg
     3121 cgagatcgca gtcgataagg cgggaatgaa gaaagcagac gtccagttct tcctctgcgg
     3181 cgatctgatg agtcagatta tttccagcag ttttgccgca cgaaccctgg gagcgccgtt
     3241 tttggggttg tatggagctt gttccagctc aatggaggga atggccattg ccgcttctct
     3301 aatcgacggg ggtcacgccc aaaatgcctt ggtcgccacc tccagtcata atgcggccag
     3361 tgaaaaacag ttccgatatc ctaccgaata cggcgcccaa aaacccccca ccgctcaatg
     3421 gaccgttacc ggcgccggag ccgccatctt gacgggggag ggagaaggtc cccgggtaac
     3481 tgcggcgaca atgggacggg tggtcgacat ggggttgtcg gatccgttca atatgggagg
     3541 ggcaatggcc ccggcggctg tggataccat tgaagcccat ttcagggatt tggatcggga
     3601 cccatcctat tacgatctga tagccaccgg cgatttgggc cgggtcggac atcaaatcgc
     3661 cggcgattta ctggagaagc atggcatgag tttacctaaa gatatcttca cagactgcgg
     3721 ccttttgatc tatcgtaagg aacagtcggt gttttcgggg gcgagtggtt gcgcctgttc
     3781 agccacggtt acttacggac attttttaaa ccggatgcga aaaggggaat tgcgaaaaat
     3841 cctggtggtg gcgaccggcg cgctgttgtc acccctttcc tatcagcaaa aggagagcat
     3901 accctgcatt gcccacgcgg tagccattga aatatgaaag gggatacggg tctt
//
LOCUS       DASVMB010000201         8080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_25483,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000201 DASVMB010000000
VERSION     DASVMB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25483"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..559
                     /locus_tag="VF531_06095"
     CDS             74..559
                     /locus_tag="VF531_06095"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HEX7713573.1"
                     /translation="MVHFITGAINIGKSTRLLGIYRSLKKGDGFYNRKIYRGNVFAGQ
                     EIVHLATEESEPFSYREGFIPENWQEECRYGAFSFSRSGLEFGREIIKKALCFRIEPL
                     FIDEIGPLELQGEGFHDIFSSLINTSVEIYVVVRQNSLDDVIREFPTVKFMLIDAAEY
                     K"
     gene            659..817
                     /locus_tag="VF531_06100"
     CDS             659..817
                     /locus_tag="VF531_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713574.1"
                     /translation="MNYQLQRTAEEGQKTAARVGNPKLIKENKEDAHVIAIPHDSRQT
                     ALRHDETR"
     gene            837..1499
                     /locus_tag="VF531_06105"
     CDS             837..1499
                     /locus_tag="VF531_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7713575.1"
                     /translation="MIQKTNRSITVDRQKEERKERVVLAAIKVFTEKGIENAKMTDIA
                     EKAGIGVASVYRYFKTKPELVIAAASKFWELGFALLPGNDDQVVLSQLNGLEKVRRIL
                     EVSLTLFRERQDFIRFLEELDYYILKEQIPVDQLAVYEMDMFDTQTLLDAALETGKRD
                     GSIRPDIDINQFSSIITRALMSYCQKLVLRGNVVPSDREFSGELQIEVLIDMAIRYIE
                     KK"
     gene            1519..3291
                     /locus_tag="VF531_06110"
     CDS             1519..3291
                     /locus_tag="VF531_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEX7713576.1"
                     /translation="MKIESGRLIYTAGNRKKVCSFQGDLDDEEVEIHYTTSQVEGGQR
                     IGLKIVPKKAIKINRLYLDTDLHLSQEDTIFLNGYQSWTESREFNPGERIPRLNRIAR
                     LMLERYGDYGFYRPRGRVLQSWTYTYIRNRNGIDFAGSLSENTGYTVFEYYQRKHLRV
                     VKDCKGLHINSPYDVFDLFVATGEENQVFDSFFGLMNLPKPKIGPCTGWTSWYNYYSK
                     ISEDVVLDNLAAFCEKSIPIDVFQIDDGYQNAVGDWLVINPKFPQGMKYMASEIKKCG
                     YKAGLWLAPFACEKRSELYQNHPEWIVTKAGYNPGWKGYFYALDFYNPEVRAYLTKVF
                     RVVFEEWGYDLVKLDFLYAAALQERKDKTRGQIMHEAMTFLRELAGDKLILGCGVPLG
                     SAFGLVDYCRIGSDVALSWEDRFLRRVHYRERVSTINSLTSTIGRRHLNGRGFYNDPD
                     VFVLRSSNNQFIKEQRHTLFLLNRIYGSVLFTSDNINEYTSEEMAMYRSLFPLVPSKI
                     RKVNTQKNLVKTYFSVGNDQYLVVSNLGDKKTNFITENECVSKGQGLLPKGAVVTLNP
                     YQSICFIYPKPGVERLSGYCKCIV"
     gene            3369..4130
                     /gene="ubiE"
                     /locus_tag="VF531_06115"
     CDS             3369..4130
                     /gene="ubiE"
                     /locus_tag="VF531_06115"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7713577.1"
                     /translation="MDHNRTWERPDLPGEKALIRIKATEMKEIFDRIARRYDLANTIM
                     SMGRHHYWRRSAVSRTELKSKGRALDLCCGTGMITADLAKQVGPDGRITGMDLSGEML
                     AIARRHLQKWGLLHQVQLVQGDAGKLPFRKDSFDCVTIGYGLRNVPDPVKVLQEAFRV
                     LKPDGRIVVLETAKPLNPFFGKAYFFYLRNWIPLVGKILCHQPAAYQYLHDSIAEFFT
                     PNEVIRFFYRAGFEDVDCTLLTLGIVGVYTGRKKR"
     gene            complement(4127..5446)
                     /locus_tag="VF531_06120"
     CDS             complement(4127..5446)
                     /locus_tag="VF531_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713578.1"
                     /translation="MEKTGIPSHFPTSVQDWLNYTLLKTGDQQIRAIIRFSGRIDSDL
                     LSRAIRLTLDAEPVLGCGFNPHPWKPIWRRIDNLDSIKHLTIVSSDNIDQEIAGFMGY
                     RIDPQYGPQVKAKVIRSTNDTLLLAINHMVADGTGVKEYLYLLADIYNKLKSNKNWRP
                     VPNLKDRGQAQLLKQIRFRNKCRSLLFGHYPKQSWGFPYRNRDTSNPTYALKSIYPQD
                     FKAIQQYAKAKQITLNDLFVTAFYNSLFHFVQPNEGDAVPMLITVDLRRYLPSKKTET
                     ICNLSGSLFISLRYQRELSFEEVLRQVKNGITTEKKHYPGLGMAFLMECISYFGFQMF
                     QKRSDKLTKDAMITGNAYPTPSNLGVIDSDLLWFKDITVDSAWIMGPTPYPPAFLVLI
                     STFKEQVTFCSAYCKNAVAEGMVENLLERMVDDVRRQTSEFERSIRS"
     gene            5626..5850
                     /locus_tag="VF531_06125"
     CDS             5626..5850
                     /locus_tag="VF531_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713579.1"
                     /translation="MNTPGHSDDSISFVMYLEGAFTGDLHPTNQVSEDLLGVVNESWR
                     KIKQTGLKVIFPGHGPSFNIDNSEPPFSSI"
     gene            6026..6628
                     /locus_tag="VF531_06130"
     CDS             6026..6628
                     /locus_tag="VF531_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713580.1"
                     /translation="MVTNDASSERPRIPLRDFFKNPLKTAFQISPDGNYLSFMGPYQN
                     RMNIFVQQRGVHEAIRVTGETARDITDYFWKGNNRIIYRKDFKGNENYHLFSVDRDGR
                     NLKDLTPFEGVRAEIIDKHTIHETEMLIGLNKRDPQVFDAYRLNVKIILNHFQGCTLY
                     PIFTISALRTLSAALKVPSFCTAKTATRILGSGVVNCTSN"
     gene            7288..8055
                     /locus_tag="VF531_06135"
     CDS             7288..8055
                     /locus_tag="VF531_06135"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713581.1"
                     /translation="MQNLIEVVNLGMKYHSLNGEITALERVDFTVKKGEFVCIVGPSG
                     CGKSTLLSMIDGLLFPTSGQILIEGIPVTGTSSKVGYMLQKDYLFEWRTIMENVLLGL
                     EIRKALTPQSKEYVKQLLKTYGLYDFKDKYPQQLSGGMRQRAALIRTLAIQPEILLLD
                     EAFSALDYQTRLLVTEDIYGIIKQENKTALMVTHDIAESISMADRVIVLSERPAVVKN
                     IYDIHLTCEKRTPICSRNAAEFKLYFNQIWKELDVHA"
BASE COUNT         2267 a         1625 c         1933 g         2255 t
ORIGIN      
        1 tttttccttg agacatgttt tacttttcct aaaatatgat gagctaaatc ttatttaagg
       61 tggtatctgc gaaatggtcc attttattac cggggctata aacatcggca agtctacccg
      121 tttacttggt atctacagga gtcttaaaaa gggggacggt ttctacaatc gcaaaattta
      181 ccggggaaat gtgtttgcgg gtcaggagat cgttcatctg gccactgaag aaagcgaacc
      241 gttttcctat cgggaaggtt ttatccccga aaactggcaa gaggaatgcc gctacggtgc
      301 ttttagtttt tccaggagcg gtttggagtt tggccgggag atcatcaaaa aggcgctttg
      361 ttttcggatc gaaccgttgt ttattgatga gatcgggcca ttggaactgc agggagaggg
      421 gtttcatgat atcttctcaa gtttgataaa tacttcagtg gagatctatg tcgtggtgag
      481 gcaaaattct ttggacgatg tcattcggga atttccaact gtgaagttca tgcttattga
      541 tgcggcggaa tataagtaac ctatcatacc gcaaacttta attatcgaaa acttcggcga
      601 aaaaatatga atgtattgtt ttgccaaata attctggtat aatatatagg gttgatagtt
      661 gaactatcag ttacagagga ctgcggaaga aggccagaaa accgctgcta gagtgggaaa
      721 tccaaaattg atcaaggaaa acaaggaaga tgcccatgtg atcgccatac cccatgatag
      781 ccgccaaaca gcgctaaggc atgatgaaac gcgataatct aaggagctct atcgggatga
      841 tccaaaaaac gaaccggagc atcactgtcg accggcaaaa agaagaacgc aaagagagag
      901 tcgttttagc cgccataaag gtatttacag aaaaaggcat tgaaaatgca aaaatgaccg
      961 atattgccga aaaggcggga atcggcgtgg catcggtgta ccgttatttc aagaccaaac
     1021 ccgaattggt cattgccgcg gcatccaagt tctgggagtt gggttttgca ttgttgcccg
     1081 gtaacgatga tcaagtggtc ttaagtcaat tgaacggttt ggagaaggta cggcggattc
     1141 ttgaagtttc tttgaccttg ttccgtgaac gccaggattt tatacgattc cttgaggagc
     1201 tggattatta cattcttaag gaacagatcc ctgttgatca gttggcggtt tatgaaatgg
     1261 acatgtttga cacccaaacc ttgctcgatg ccgcactgga gacgggaaaa cgcgacggtt
     1321 ctatccggcc ggatatcgac attaatcagt tttccagtat tatcacgcgt gccttgatga
     1381 gctactgcca aaagctggtt ttgagaggga acgtagtacc cagcgaccgg gagttttcag
     1441 gcgagcttca gatcgaagta ttgatcgata tggctatccg ttatattgaa aaaaaataga
     1501 acccggggtg attgagggat gaagattgaa agtggccgtt tgatttatac ggcggggaat
     1561 agaaaaaagg tatgcagctt tcaaggtgat ttggatgatg aagaagttga aattcactat
     1621 acaacttcac aggtagaggg cggtcaaaga atcggattga aaatagtgcc caaaaaagcg
     1681 atcaagataa acaggttata tcttgataca gatcttcatt tatcacagga agataccatt
     1741 tttctcaacg gctatcaaag ttggaccgaa agcagagagt tcaatcccgg tgaaagaata
     1801 cccaggctta acagaattgc ccggctgatg cttgagaggt atggggatta tggtttttac
     1861 cggccacgcg gcagagtcct gcaaagttgg acctatacgt atatccgtaa tcgtaatggg
     1921 attgatttcg ccggttcgtt gtcggaaaat accggttata ctgtcttcga gtattatcag
     1981 cgtaagcatt tgcgagtggt caaggactgt aaaggtctac acatcaatag cccgtatgac
     2041 gtctttgatt tgttcgttgc cacgggtgag gagaatcaag tattcgacag cttttttggg
     2101 ttaatgaatc tgccaaagcc taaaataggt ccctgtacgg gttggaccag ctggtacaat
     2161 tattactcga aaataagcga agatgttgtc ctggataatt tggccgcatt ttgtgaaaaa
     2221 agcataccta ttgacgtgtt ccagatagac gacggttatc agaacgctgt gggtgattgg
     2281 ttggtgatca atcccaagtt tcctcaaggt atgaagtata tggcttcaga gattaaaaaa
     2341 tgcggttata aggcaggtct gtggctggcg ccattcgcct gtgaaaagcg ctcggaactt
     2401 tatcaaaatc acccggagtg gattgtaaca aaggcaggct ataatcccgg ctggaaaggt
     2461 tatttttatg cgctcgattt ttacaacccg gaggtgcggg cttaccttac caaggttttt
     2521 cgggtggtat ttgaggaatg ggggtatgat ctggtaaagc tggactttct ttatgccgcc
     2581 gcgttacaag aacgaaaaga taagacccgc ggccagatta tgcatgaagc catgactttc
     2641 ctgagggagc ttgccggtga taagttgatt ttgggatgcg gggtacccct gggctccgcc
     2701 ttcggcctgg tagactactg ccgaatcgga agtgatgtgg cgctttcctg ggaagaccgg
     2761 ttccttagac gggtgcatta ccgggaaagg gtttccacca taaactcact gaccagtacc
     2821 atcgggcgca ggcacctcaa cgggaggggt ttttataatg accccgatgt cttcgtttta
     2881 cgaagcagta ataatcagtt tatcaaagaa caacgccata cattgttttt attgaatcgc
     2941 atttatggta gtgtgttgtt tacttcggac aacatcaacg aatatacctc cgaagagatg
     3001 gccatgtatc gatcgctttt tccactcgta ccaagcaaga ttaggaaagt taatacacag
     3061 aagaacttag taaaaaccta tttttcggtg gggaatgatc aatatctggt tgtcagcaat
     3121 ctgggcgata aaaaaacaaa tttcattacc gagaacgaat gtgtatccaa gggccaagga
     3181 cttctcccca aaggtgccgt ggttaccctg aacccttatc aatccatatg ctttatctac
     3241 ccaaaacccg gggtggagag attaagtgga tattgtaaat gtattgttta aacatattcc
     3301 ttgtgtgatc agatcaaact atattgaatg cgccggcatt caataataaa ccatgagagt
     3361 tgaggaatat ggatcacaac cgtacttggg aaaggcccga tctacccggg gaaaaggcat
     3421 tgattcgaat caaagcgacc gagatgaaag agatttttga ccggattgcc cggcgttatg
     3481 atttggcaaa taccatcatg tccatgggca ggcatcacta ttggcgaagg tctgcggttt
     3541 cccggacgga gcttaaatcc aaaggaaggg cacttgacct ttgttgcggg acggggatga
     3601 ttactgccga tttggcaaaa caggtcggcc cggatggacg aattaccgga atggatctat
     3661 caggggagat gctggcgatt gccaggaggc atctacaaaa atggggtttg cttcatcagg
     3721 tgcaattggt tcaaggagat gccggtaagt taccctttcg aaaggattca tttgattgtg
     3781 tcaccattgg gtatggttta cggaacgtgc ccgatccggt aaaggtgctt caggaggcat
     3841 tccgggtgct caaaccggat ggcaggatcg tcgtcttgga aaccgccaaa ccattaaatc
     3901 cgtttttcgg caaggcctat tttttttatt tacggaattg gatcccgttg gttggaaaga
     3961 tcctgtgcca tcaaccggcg gcataccaat atctgcatga ttccattgcc gagttcttca
     4021 cacccaatga agtcatccgt tttttttaca gggccgggtt cgaggatgtc gactgtactt
     4081 tattaacatt aggaattgtt ggagtttata ccgggaggaa aaaacgttag cttcttatgg
     4141 atcgctcgaa ttcagatgtt tgccttcgta catcatccac cattcgttcc aatagattct
     4201 ccaccatacc ttcggccact gcatttttac aataggctga acagaaagtt acctgttcct
     4261 taaaagtact gattaatacc agaaatgcgg gtggatacgg agtcggcccc attatccacg
     4321 cactatcaac tgtaatgtcc ttgaaccata ataagtctga gtctatgact cctagatttg
     4381 atggcgtggg ataagcatta ccggttatca tggcatcttt cgttaattta tcacttcgct
     4441 tttgaaacat ttggaatcca aaatatgaaa tgcattccat caaaaaagcc attcccaaac
     4501 cggggtaatg cttcttttcc gtagttattc catttttgac ttgacgtaaa acttcttcaa
     4561 atgaaagttc tctttgatag cggagagaaa taaataatga accggaaaga ttgcagattg
     4621 tctccgtctt tttggaaggt agatagcgcc gtaagtcaac ggtgattaac atcggcacgg
     4681 catctccttc gtttggttgc acaaaatgaa acaaagaatt gtaaaatgcc gttacgaaca
     4741 ggtcgtttaa ggtgatctgc ttggccttgg catactgttg gatcgcttta aaatcttgtg
     4801 gataaatgct ttttagcgca taggttggat ttgatgtatc ccggtttcgg tagggaaacc
     4861 cccaagattg tttggggtaa tgcccgaaga ggagagaccg acatttattc ctgaatcgaa
     4921 tttgcttcag tagttgtgcc tggcctcgat ctttgagatt tggtaccggt ctccaattct
     4981 tattcgattt cagcttgtta taaatatcgg ccaataaata aagatactct tttactcctg
     5041 ttccatcagc aaccatgtga ttaatcgcaa gtaaaagcgt atcgttagtc gagcgtatca
     5101 ctttagcttt aacctgtgga ccgtactgag gatcgatcct gtagcccata aaaccggcga
     5161 tttcttgatc gatattatcc gatgacacga ttgttaagtg ctttatgctg tcaagattat
     5221 ctatccgccg ccaaatgggt ttccatggat gcggattaaa accgcaaccc aaaacaggtt
     5281 ccgcatccaa tgtcaaacgg atagcccggg atagaagatc ggaatcaatt cttccgctaa
     5341 accgtataat cgcccttatt tgttgatctc cggtttttaa caatgtgtaa ttcaaccagt
     5401 cctggacaga tgtgggaaaa tgtgatggaa tcccggtttt ctccatggtt cgtttaattt
     5461 tcctttcaaa aaactttatc gaaactatat taacatttct atgttatatg acgattccct
     5521 ttattgcttc gattactcgg ttaaaccggt acaaccaggg tcaccgtcat ttcctccggg
     5581 aacagccggt ctttcttttt caaactaaac atggacgccg aatttttgaa cacacccggt
     5641 cattccgacg acagcatcag ttttgtgatg tatcttgagg gtgcttttac cggcgattta
     5701 cacccgacca atcaagtctc tgaggatctt ctcggtgtgg tgaatgaaag ctggcggaag
     5761 atcaaacaga ccggactaaa ggtgattttt cccggacacg gtccgtcttt caacatagat
     5821 aacagtgagc cgccattttc atcaatataa acacctgaaa attgtttatc aattatcaag
     5881 ggagtaaaga agcatacgct tcattacccc ctgtttttaa tggaggtacc ggtctactca
     5941 taaaaccatc aattaactct tgggtatggg gactcttatg ccatcggcgc catatttata
     6001 gttatagatc aaggaggcga taaagttggt tacgaatgat gcaagttcgg agagaccccg
     6061 gatcccattg cgcgattttt ttaaaaaccc gctaaagaca gcattccaga tttcaccgga
     6121 cgggaactat ctttccttca tgggacctta ccaaaaccgc atgaatattt ttgtacaaca
     6181 acggggcgtc catgaagcga tacgggtcac tggagaaacc gcccgcgaca ttacagatta
     6241 tttttggaaa ggtaacaacc ggattattta ccgtaaagat tttaagggca atgagaatta
     6301 tcatctgttt tctgtggatc gcgatggccg gaatttaaag gatctgactc cttttgaagg
     6361 tgtacgggcg gaaatcatcg ataaacatac cattcatgag accgaaatgt tgattggttt
     6421 aaataaacgc gacccccaag tctttgatgc ctatcgtttg aatgtaaaga taatcctgaa
     6481 ccatttccaa ggatgcaccc tttatcctat ctttaccatt tctgcgcttc ggacgctgtc
     6541 tgcagcgcta aaagtaccgt cattctgcac tgcgaaaacg gcaaccagga tattggggtc
     6601 gggagtggta aattgcacct caaactgaag taaggcgggg atgtttatga ccgcaaagtc
     6661 ggatgattcg ggttgaagta taaaggtacg tgtggtaaat actcttttcg taccgtccaa
     6721 atcgaatatt gtcgctgtga catttgcaac cgaacttgaa ttattaagta cctttagacg
     6781 taattgaact acattgcctt cctgttcaat cgggcctgtt gaaaggataa acatattgaa
     6841 aaaaccccct tccattttaa acataggtaa gtgaggcgaa gaaggcttgg aaatgtttga
     6901 tttttgatca tcttcaaagt gcggctaata aatgtcatga gatcatgtac tgccattgga
     6961 agggatgaat tcggaaagaa tctcccttct agttaatcag gttatgtttg cgattggttt
     7021 atgaaacctg caaaatgaac aagatatcga gttattatct catttctttg gataaactct
     7081 ttgaacgggt attgtggagg gaatgataat ttttggtaaa aaatcattgg catgcagcca
     7141 ataaagtcag caaagccgct tgcaacggct tttttatttt cacttgttaa aacgtagccc
     7201 catcaaaaac ggttaccccg aggctaccgg gcgaatatcc tataagcaag tcgaaggaaa
     7261 tggcatttag gtttgaggtg attcttactg caaaacctga tcgaagtagt aaacttggga
     7321 atgaaatacc actcgctaaa cggggaaata accgccctgg agcgggttga ttttacggtc
     7381 aaaaaaggcg agtttgtatg catcgtcggg ccgagtggtt gtgggaaatc aaccctcctt
     7441 tcgatgattg acgggttatt atttccaacc agcggtcaga ttttgattga aggcataccc
     7501 gtaacgggca cgtcatcaaa agtgggttat atgctccaaa aggattacct ttttgaatgg
     7561 cgcaccatta tggagaatgt ccttttgggg ctggagatcc gcaaggcgct gacaccgcaa
     7621 tccaaggaat atgtgaagca attactgaag acctacggat tatatgactt caaagataag
     7681 tatccgcagc agctctccgg gggcatgcgg caacgggcgg cgctcatccg gacgctggcg
     7741 attcaaccgg agatattact cctggatgag gcgttttcgg ctttggatta tcagacccgt
     7801 ctactggtga ccgaagatat ttacggcatc atcaagcaag agaataagac ggccctcatg
     7861 gtcacccatg atatcgccga aagcatcagc atggcggatc gggtgattgt cttatcggaa
     7921 agacctgccg tagttaaaaa catttatgat attcatttga cttgtgagaa gcgcacaccc
     7981 atttgctccc ggaatgcggc tgaatttaaa ctgtatttca accagatatg gaaggagctt
     8041 gacgttcatg cttgataagc caggcaacaa gtcggtggcg
//
LOCUS       DASVMB010000202         8458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_393327,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000202 DASVMB010000000
VERSION     DASVMB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393327"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2470
                     /locus_tag="VF531_06140"
     CDS             <1..2470
                     /locus_tag="VF531_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713582.1"
                     /translation="LCGNEVFHFPFVIDDTGLLVNGFTDPVNKRIHVFEYPPDQASSL
                     GATENWWSMVGVHEYTHELSLTKTTGSNKILQDVFGNDSFWMPNVSALPGWVFEGITV
                     YSESQLTPYQGRLNDGYFDSYIGARVRDGRFPSILDATFEPIEFQASGIYTYGGEFFN
                     YLSNVYGPEKFARFFEVNGESIPLVSWLYPIFGADRSARKVYGKSLPELWKDWKEYEI
                     EHYRDYRTDGEVVPGSAGSVIQIYFSEDMLYYERQITAKTGAFQDSSYYELVGRNLES
                     GRERILVSTHAPFTMSIRVKNGKLYYGTHETKTGYANSSQNSIGYYALIHRYDLESGR
                     DQVIFSGPIRAYDISANGEIILSEDRIGRFGSMIYQFNPATGEKRQLFEGDFLVDEIV
                     SDVKRTVTAARKDWQNFNIYQLDLETGSFKPLVATPYSEGNITLQGERLLFNANYSKQ
                     VAGYSYDFSNGKVYRLTENGYAANPILDEPHQELYYIGLSSYDFTLNRKPVDFKEYQV
                     PALQEPTPPIISLPQTAVTRGGYWDNLKTLAPLSWMPIVNVDENRSEYGVSLTGGDAV
                     MDFPFYMLELSYDAQRKKVNSNLNMTVNYFAPWQISLKYSNVDKNFLALGMAYPLVTR
                     LSDGLTDLSIGTSMEYQADFPGLTWDPFVKAGWEWPRTSMQFKIDTPLGKTQKGDVRN
                     GWYTDLEIKRTFTESQLTLDASRIIDPANPHTVLPMIRGYSEALPDKDGQVYSLEYSQ
                     PVLKIRNGFWNPNIYFEDLFATAFVDQAVASSGDRQAAWGLELHLETKMMYDKMSPLD
                     WGLRITQNQEGEECFNLFFRSVVN"
     gene            complement(2780..4975)
                     /locus_tag="VF531_06145"
     CDS             complement(2780..4975)
                     /locus_tag="VF531_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HEX7713583.1"
                     /translation="MAVSKYTEQKIGDTLEQNYMPYAMSVIISRAIPEIDGFKPSHRK
                     LLYTMYKMGLLNGGRTKSANVVGQTMKLNPHGDMAIYETMVRLTRGYDALLHPFIDSK
                     GNFGKQYSRDMRFAAPRYTEAKLDKICEELFRELDRDAVDLIDNYDGTLKEPTLLPTT
                     FPNILVNANQGIAVGMASNFCSFNLAEVCETTIQYLQDENIKLTSYLKAPDLSSGGQL
                     IYNEADLTEIYETGRGSFKLRARYRYDQANNCLEIYEIPYTTSSEAIIDALVELVKNG
                     KIKEITDIRDETDLNGLKITLDLRKNSDPDGLMNKLYRLTPLEDSFNCNFNLLINSRP
                     KVLGIRAILGEWVNFRIECLKRETRFDLDQKSKQLHLLQGLQQILLDIDKAIRIIRQT
                     DQESQVIPNLMQGFAIDQVQAEFIAEIKLRNLNKEYIMNRIGEITRLQKEIASLKELL
                     NSESRQKQLIISQLREVSKKYGKPRRTELINEARIEPITTEHLIEDYNLKIFLTEQNY
                     LKKVPLTSLRANPEQKLKEDDRIVAEIETHNKADLLLFSNRQTVYKMKIYEINDCKAS
                     SLGEFLTNLLALEPEEKILYIVATDNYSGKLLFAFENGKIAKIDLESYATKTNRRKLA
                     NAYSDLAPLVSFYYLQEDIDLVAFSSIKKVLIFNTAQLSAKTTRDSQGVQVLTAKKDS
                     RMIALKPLSEVHLTDPDYYRTKNIPAIGCYLKDEDNPERQVGLQLEIKQ"
     gene            complement(5053..7038)
                     /locus_tag="VF531_06150"
     CDS             complement(5053..7038)
                     /locus_tag="VF531_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7713584.1"
                     /translation="MAMDTRRKAEDYGNDSISSLKGAARVRLRPGVIFGSDGLEGCEH
                     SVFEILANSIDEAREGFGNIIEIIRHQDGSITVKDEARGVPVDYNPIEKRFNWELVYC
                     ELYAGGKYKNNSGENYEFSLGLNGLGCCATQYSAEFMDVTVYRDGYRYTLHFEKGENS
                     GGLHKEKYRYDHTGTITHWRPDRQVFTDTAIPLEYFQTTLKKQAVVNAGLTFRLLDEV
                     SGESFEYCYQNGIVDYLKEISGDQNISEVQYFETSTKGRDREDKPEYKVKMQVAFCFN
                     NQVNLLEYYHNSSFLEYGGAPDRAVKNAFVYEIDRTLRARNKYTKDEAKITFADIQDS
                     LILVTNSFSTETSYENQTKKSITNKFIQEAMTDFLKAQLEIYFIENMAESERLLDQVL
                     VNKRSRETAERTRINIKKKLSGNADLTNRVKKFVDCRTKDLNLREIYIVEGDSALGSC
                     KLGRDAEFQAIMPVRGKILNCLKAEYDSIFKNEIIVDLLKVLGCGVEIKSKHNKDLNT
                     FDLNNLRWNKIIICTDADVDGFQIRTLILAMLYRLVPTLIQAGKVYIAESPLFEISTK
                     GNTYFAYSEKEKQEILARVKGKYSIQRSKGLGENEPEMMWQTTMNPETRRLIKVVPED
                     LERTEQLFDLLLGDNLQGRKEYIETNGYRFLDMIDVS"
     gene            7309..>8458
                     /gene="araA"
                     /locus_tag="VF531_06155"
     CDS             7309..>8458
                     /gene="araA"
                     /locus_tag="VF531_06155"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX7713585.1"
                     /translation="MGNLKSRMNSTIGLQGKEVWFVTGSQHLYGEEPLRQVAEHSQLI
                     AQSLAEHEEVVVPLRFKAVLTTPEAIRQLCLDANNSENCIGIIAWMHTFSPARMWIAG
                     LNILHKPLAHLHTQFNRDIPWSEIDMDFMNLNQSAHGDREFGFINTRLSKARKVIVGF
                     WKDPEVVERLASWIRTALAWNDLQGAKFARFGDNMREVAVTEGDKVEAEIRLGYSVNG
                     YGVGDLLEHVKGVAEPELQQLLEEYESEYQLQKELAKGGAKRESLVEAARIELGLKHF
                     LEAGGFKGFTTTFEDLHGLAQLPGLAVQRLMAAGYGFGAEGDWKTAALVRAMKVMGYG
                     MAGGTSFMEDYTYHLAPGDQKILGAHMLEVCETIAAHQPSMEIHPLSIG"
BASE COUNT         1991 a         1981 c         2177 g         2309 t
ORIGIN      
        1 gctctgcggc aatgaagtgt tccattttcc attcgtcatc gacgacaccg gattactggt
       61 gaatggtttt accgatccgg taaataaacg gattcatgtt tttgaatacc cgccggatca
      121 ggcaagttct ttgggagcta ccgaaaattg gtggtctatg gtaggagtcc acgaatatac
      181 tcacgaactt tcgttgacaa aaacaacagg ttcaaacaag attcttcagg atgttttcgg
      241 caatgattcc ttttggatgc ccaatgtttc tgcgctgccc ggatgggtct ttgaggggat
      301 caccgtttac agtgaatcgc agctaacgcc ttatcaggga aggctgaatg atggttattt
      361 cgactcgtat atcggggccc gggtccggga tggacgtttt ccttccattt tggatgcgac
      421 ttttgagccc attgagtttc aggcttccgg gatatatacg tatggcgggg agttctttaa
      481 ttacctgtca aacgtttatg gaccggagaa attcgcacgg tttttcgaag tcaacggtga
      541 gtccattccg ctcgtttcct ggctttaccc gatcttcggc gccgaccgga gcgcccggaa
      601 ggtctacggt aaatcccttc ccgagctttg gaaggattgg aaagaatatg agatcgaaca
      661 ttaccgggat taccgaaccg atggggaggt ggtgcccggc tccgccggaa gcgtaattca
      721 aatctatttc agcgaagata tgttgtatta tgagcgacaa atcactgcaa aaaccggagc
      781 ttttcaagat tcgagttatt acgagcttgt ggggcgaaat ttggagagcg gcagggaacg
      841 gattctggta tccactcacg ccccttttac catgtcgatc cgggttaaaa acgggaaatt
      901 gtattatggt acccatgaaa ccaagactgg ttacgccaac tcttcccaaa acagtatcgg
      961 atattatgcc ttaattcatc gatacgactt ggagtccggg agggatcaag taattttctc
     1021 cggcccgata cgggcttacg atatttcggc taacggtgaa atcattttat cggaagaccg
     1081 gattggcaga ttcggctcga tgatctacca gtttaatccc gctaccggtg agaaacggca
     1141 gctctttgag ggtgactttt tggtggatga gatcgtaagt gacgttaagc gaaccgtgac
     1201 ggcagccagg aaggactggc agaatttcaa catctaccaa ctggatctgg aaaccggatc
     1261 tttcaaacca ttggtggcta caccctattc tgaaggcaat atcacgcttc agggcgaacg
     1321 attgttattt aacgccaatt attcaaagca agttgccggg tattcctatg atttctcaaa
     1381 tggtaaggta taccggttaa ccgagaacgg ttatgccgca aacccgattt tggacgagcc
     1441 gcaccaggag ttgtactata tcggcttaag ctcttatgac tttaccctaa accgaaaacc
     1501 ggtggatttt aaggaatatc aagtacctgc actccaggaa cccacccccc caatcatttc
     1561 cttaccgcaa accgctgtca ctcgcggcgg atattgggac aatctaaaga ccttggcgcc
     1621 gttatcctgg atgcccatcg tgaatgtgga tgagaaccgc agcgagtacg gtgtctccct
     1681 gacgggcggc gatgccgtta tggattttcc attttatatg ctggagttgt cctatgatgc
     1741 ccagcggaag aaggtgaatt cgaatttaaa tatgacggtg aattatttcg cgccatggca
     1801 aatctcgcta aaatactcca atgtcgataa aaactttttg gccttgggta tggcctaccc
     1861 gcttgttact cgtctatccg acgggttaac cgatttgtcc atcgggactt ccatggagta
     1921 tcaagcagat tttcccggtc tgacttggga tccgttcgtg aaggcgggat gggagtggcc
     1981 caggacttcg atgcagttta aaatcgatac tcccctgggg aaaacccaaa aaggtgatgt
     2041 ccgaaacggt tggtacacgg acctcgaaat taaacgaaca tttactgaaa gtcaactgac
     2101 tcttgatgcg agccgtatca tcgatccggc aaacccccat accgtactac cgatgattcg
     2161 cggttatagc gaggcattac cggacaaaga tgggcaagta tacagcctcg agtattccca
     2221 gccagtactc aagatccgta atggtttttg gaatcccaat atttatttcg aagatctttt
     2281 tgcaacagcc tttgtcgatc aggcagtggc atcaagcgga gatcgtcaag cggcctgggg
     2341 attggaattg cacctggaga cgaaaatgat gtatgataaa atgtcaccac tcgattgggg
     2401 attacggatc acccaaaatc aagaaggcga ggaatgcttc aacctctttt tcaggagtgt
     2461 ggtaaactag gcaagaggga acaggcatga ggcatgaggc ataaggcata aggcatgagg
     2521 actttcgggt cttgtggata tgttctgtag tttgggtcta tagcctttgg accttggatt
     2581 ccggcgggtt ccctttaagg gaaagggtag ataggctgag attaggtcca attaccctag
     2641 cccattaaag tagagttgta ttacaactct taagaaaccg gttcaccgtt ttcaggggac
     2701 cagcatgact cccgggtatt gttggctata cttcatagct aaaacccccg gccttttgac
     2761 ccgggattcc ggctggtttt tactgtttga tctccagttg caaaccgact tgtcgttccg
     2821 gattgtcctc gtccttcaga taacaaccga tggccgggat gttcttggtc cggtaataat
     2881 cgggatcggt taaatgtacc tcactcaagg gtttcaaggc tatcatccgg ctgtctttct
     2941 tagcggtcag cacttggaca ccctgggaat cgcgggttgt tttggcactc aactgggcgg
     3001 tgttgaagat caaaaccttc ttgatgctac tgaaggcgac cagatcgata tcttcttgca
     3061 gatagtagaa tgagacgagc ggcgccagat cggaataggc atttgccagt ttgcgtcggt
     3121 tggtcttggt agcgtaactc tccagatcga tcttggcgat cttgccgttt tcgaaggcaa
     3181 agagaagctt gcccgaataa ttatcggtcg ccacgatata gaggatcttc tcctccggtt
     3241 ccagagctag caggttggtg aggaactccc ccaaactgct tgccttgcag tcgttgatct
     3301 cgtatatttt cattttgtaa acggtttggc ggttggagaa gagtaaaaga tccgccttgt
     3361 tatgcgtctc aatctcggcg acaatcctgt cgtcttcctt gagtttctgt tccgggttgg
     3421 cccggagcga tgtcaaggga acctttttca gataattttg ctccgtcagg aagattttca
     3481 gattgtaatc ctcgattaag tgctcggtgg tgatgggttc gatccgggcc tcattgatca
     3541 attcggtccg ccgcggtttc ccgtatttct tggaaacttc ccgtaactga ctgatgatca
     3601 actgtttctg ccgggactca ctgttcagca gttcttttaa agaggcaatc tctttttgaa
     3661 gccgggtgat ctcgccgatc cggttcatga tatattcctt gttgaggttg cgcagtttga
     3721 tctccgcgat gaactcggcc tgaacctgat caatggcaaa tccctgcatt aaattgggta
     3781 tgacctggct ttcctgatcg gtctggcgga taatgcggat cgctttgtcg atatccagta
     3841 gaatctgctg aagtccttgg agtaagtgca attgtttcga tttttgatcc agatcgaagc
     3901 gtgtctcccg cttcaagcat tcaattcgga aattcaccca ttccccgagg atggcgcgga
     3961 tccccagtac cttcggacgg ctgttgatta gcagattaaa gttgcagttg aagctatctt
     4021 ccagcggcgt gagccggtaa agcttattca tcaagccgtc gggatcactg tttttgcgga
     4081 ggtccagggt gatcttcagg ccatttaggt cggtttcatc ccgaatatcg gtgatctcct
     4141 tgatcttgcc gtttttaacc agctccacca gggcatcaat gatggcctcg gaagacgtgg
     4201 tatagggaat ttcatagatc tccaagcaat tattggcttg atcataacgg taacgggccc
     4261 ggagtttgaa actaccgcgt ccggtttcgt aaatttcggt gagatcggct tcattataga
     4321 ttagttgtcc cccggaagag agatccgggg ctttcaagta cgaagtgagc ttgatattct
     4381 cgtcctggag atactgaatg gttgtttcac agacttcggc caggttaaaa ctgcaaaagt
     4441 tgctggccat cccgactgcg atcccctggt tggcattgac cagaatattc gggaaggtgg
     4501 tagggagcag ggtgggttcc ttcaaggtgc cgtcgtaatt atcgatcagg tctaccgcgt
     4561 cccggtccaa ttccctgaag agttcttcgc agatcttgtc cagtttggcc tcggtgtagc
     4621 ggggggcggc gaagcgcata tcccgggagt attgcttacc gaagttgccc ttggaatcga
     4681 tgaatggatg caatagcgca tcgtaacctc gggtcagccg gaccatggtt tcatatatgg
     4741 ccatgtcgcc atggggattt aatttcatcg tctggcctac cacattggcg gatttggtac
     4801 ggccaccgtt caagagtccc atcttgtaca tggtataaag cagtttacga tgagacggct
     4861 tgaatccgtc aatctccggg attgcccgtg agatgatgac gctcatggca tagggcatat
     4921 agttttgttc cagggtatcg ccgatttttt gctcggtgta tttcgaaacg gccatattac
     4981 tgcctcctat taacggcggt ttgtcattcc gggattttca agctttgcgc gatttccgtg
     5041 cggctactgc gtttagctga cgtcgatcat atccaggaat cggtacccgt tcgtctcgat
     5101 gtattctttc cgcccctgga gattatcgcc aagcaataag tcgaataatt gttcggtccg
     5161 ttccagatcc tctggaacaa ccttgattaa ccgccgggtt tcggggttca tggtcgtctg
     5221 ccacatcatc tccggctcgt tctcacccag tcccttggaa cgctggatcg aatatttccc
     5281 cttgaccctg gctaagatct cttgcttctc cttttcgctg tatgcaaagt aggtattccc
     5341 tttcgtgcta atctcaaaca acggggattc ggcgatataa actttgccgg cctgaatcaa
     5401 tgtgggaacc aaccggtaca gcatcgccag gatcagggtg cggatctgga acccgtccac
     5461 atcggcgtcg gtgcagatga tgatcttgtt ccacctcaga ttattcagat caaaggtatt
     5521 aaggtctttg ttgtgtttgg atttgatctc aacgccgcat cccaggacct ttaatagatc
     5581 gacgatgatc tcattcttaa agatactgtc atattccgct ttcaggcaat tcaggatctt
     5641 gccgcgcacc ggcatgatcg cctgaaactc ggcatcgcgt cccagtttac atgagccgag
     5701 cgcgctatcg ccttcgacaa tataaatctc tcgcagatta aggtctttgg tccggcagtc
     5761 gacgaatttt ttcacccggt tggtcagatc ggcgttgccg ctgagtttct ttttgatatt
     5821 gatccgggtt cgctccgcag tctcacggct acgtttattg accaacacct ggtcgaggag
     5881 gcgttcgctt tcggccatgt tctcgataaa gtagatctcc agttgcgcct ttaaaaaatc
     5941 cgtcatggcc tcctggatga atttgttggt gattgacttt ttggtctgat tttcgtaact
     6001 ggtctcggta gaaaaggagt tggtcaccag gattaggcta tcctgtatat cagcgaaagt
     6061 aatcttggcc tcatctttgg tatatttatt ccgggctctc agagtccggt cgatctcata
     6121 gacgaaagca ttctttaccg cccgatccgg tgcgccgccg tactcgagaa agcttgagtt
     6181 atggtagtat tcaagaaggt taacctgatt attaaagcag aacgccactt gcatcttgac
     6241 tttgtattcg ggcttgtcct cccgatccct gcctttggtg gaggtttcaa agtattgaac
     6301 ctcggaaatg ttctgatcgc cactgatttc cttgagataa tcaacgattc cgttttggta
     6361 gcaatactca aagctttcac cggaaacctc gtccagtagc ctgaaggtca agccggcgtt
     6421 caccaccgcc tgttttttaa gggtggtctg aaagtattcc agcggaatcg cggtatccgt
     6481 aaagacctga cgatcgggcc gccagtgagt gatggtaccg gtatgatcat agcgatactt
     6541 ctctttatga agacctccgc tattctcgcc tttctcgaaa tgaagcgtat atcggtaacc
     6601 gtcacggtag actgttacat ccataaattc cgcactgtat tgggtggcgc agcagcccaa
     6661 gccgtttagg cccagagaaa actcgtagtt ctcgccgctg ttgttcttat atttacctcc
     6721 ggcatagagc tcacaataga ccaactccca gttgaaccgt ttttcgattg gattataatc
     6781 caccggaacc ccgcgggcct catccttgac cgtgatcgaa ccatcctggt gtcggatgat
     6841 ttcaatgata tttccgaagc cttcacgggc ttcgtcgatg gaattggcga gaatctcgaa
     6901 gaccgagtgt tcacaacctt ccaggccatc cgagccaaag ataacgccag gccgtaaccg
     6961 gacccgagcc gcgcccttaa gggatgaaat gctgtcgttg ccgtaatctt ccgctttgcg
     7021 gcgtgtatcc atcgccaaaa cctgcctttt atagaatgaa atttatttct ttttccatat
     7081 ctaaaaatat accaaacaga tgttcgtgtc aagtcggtaa caacattcaa aattactata
     7141 ccatagtata gcgaatctgc aaacccaact ttcggttatt acttcctttg gatttggtta
     7201 tatgatatta ttatacatat gaaagttgta cggacaagtt gaaaaatggt cttgacatga
     7261 tcgggacttt gggccaatca tcagaaacga aaggggatca tgagagtgat gggaaacttg
     7321 aaatcaagaa tgaattcaac catcgggctt caagggaagg aggtttggtt tgtcaccggg
     7381 agccagcacc tctatggaga ggagccactc cggcaggtag ccgagcattc acagctgatt
     7441 gcccagtcat tggcggagca tgaagaagtt gtcgttccgc tccgctttaa ggcggttttg
     7501 actaccccgg aggcaatccg ccagttatgt ctggatgcga acaactcgga gaactgcatc
     7561 ggcattatcg cttggatgca taccttttca cccgccagga tgtggattgc cggactaaat
     7621 attttacata aacccctggc ccacctccat acccagttta accgggatat tccctggtcc
     7681 gagattgaca tggactttat gaatttaaac caatcggctc acggcgaccg ggagttcggt
     7741 ttcatcaaca cccggttgag caaagcccgc aaggtcattg tggggttttg gaaggacccg
     7801 gaggttgtcg aacgtttggc ctcatggatc cggacggcac tggcctggaa cgatctacag
     7861 ggtgcaaagt tcgcccggtt cggcgataac atgcgggaag tggcggttac cgagggagat
     7921 aaagttgagg cggagatccg gttgggttac tcggtcaacg gatacggagt cggtgatctg
     7981 ttggagcatg tgaaaggggt ggcggaaccg gaattacagc aacttctgga ggagtatgaa
     8041 tcggaatatc agctccaaaa ggaattggcc aaaggcggag ccaagcggga gtcactggtg
     8101 gaggccgccc ggatcgagtt ggggcttaag cattttttgg aggcgggcgg attcaagggt
     8161 tttaccacca cgtttgagga tttacacggt ttggcgcaat taccgggtct ggcggtacaa
     8221 cgattgatgg cggcgggata tggtttcggg gccgagggtg actggaagac cgcagccctg
     8281 gtccgggcga tgaaggttat gggttacgga atggcaggcg gaacctcgtt tatggaagac
     8341 tatacctacc atctggcccc gggtgaccaa aagattttag gtgcccatat gctcgaggta
     8401 tgtgaaacca ttgcggcaca tcaaccatca atggagattc atcccctttc catcgggg
//
LOCUS       DASVMB010000203         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_25617,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000203 DASVMB010000000
VERSION     DASVMB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25617"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="hemW"
                     /locus_tag="VF531_06160"
     CDS             complement(<1..741)
                     /gene="hemW"
                     /locus_tag="VF531_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7713586.1"
                     /translation="MHFPFCKQKCLYCDFNSYSGYDELIPAYLKALRMELAGYMPFAQ
                     PLGSLYFGGGTPSYLPTGLLAGLLSSILQVNGDAPREITLEANPGTLTQTSLSGLREA
                     GFNRLSLGLQATQDELLATIGRIHTWRDFCISYELARQAGFDNIGVDLIFGLPGQTAE
                     AWRDTLKMLVELAPEHISTYALQVEPGTPLETMVGSGRLRLPEEEATVAMFQTVMTYL
                     PEHGYNHYEISNYARPGFESLHNLHYWLS"
     gene            1003..1857
                     /locus_tag="VF531_06165"
     CDS             1003..1857
                     /locus_tag="VF531_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7713587.1"
                     /translation="MAFCLGSRLIVNSPLTEYLREAALQFKVIELQADPSYFSANYAL
                     TVNEKKVLRIYRERYGFQLTMHAPFINLRLGAINLEERQLAINIMINAMQAASDLEIQ
                     LVTFHPCTLEPGAPEKYHENSLLEEGSIALLLKVAKKLGITLLMENMPLVPEFHSKTT
                     DGSRFQELLWLFPEPEFGLTIDIGHALQAGIPPEGLLKMDRIRHFHLHENDRTIDNHQ
                     PITTHLEWWEKLFKTLTKKFPDAYGILEMSQFTDQIASLKELQPFLGKRALPKPGKGQ
                     MIPPLIVD"
     gene            complement(1956..>2354)
                     /locus_tag="VF531_06170"
     CDS             complement(1956..>2354)
                     /locus_tag="VF531_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX7713588.1"
                     /translation="SLDYEYLGHRQSELVKLDILLNEKVVDALSAIVHRDKAYSRGRQ
                     LAEKLKDIIPRQLFEVPIQAAIGTKVIARETVKALRKDVLAKCYGGDISRKRKLLEKQ
                     KEGKKRMKQLGSVEVPQEAFLAVLKIDDET"
BASE COUNT          608 a          603 c          562 g          581 t
ORIGIN      
        1 gctcaaccaa taatgaagat tgtgtagcga ttcaaatccc ggccgggcat aattggagat
       61 ctcgtaatga ttgtagccat gctccggaag gtaagtcatt accgtttgga acatggccac
      121 ggtcgcttct tcttccggta aacggagccg gccgctcccc accattgtct caaggggggt
      181 tccgggttct acttgcagcg cgtaagtaga gatatgttcc ggggccaatt ccaccagcat
      241 ttttaaggta tcgcgccatg cctcagcagt ctgcccgggc aatccgaaga tcagatccac
      301 tccgatgtta tcaaatccgg cctgacgggc cagttcgtaa ctgatgcaaa agtcccgcca
      361 ggtatgaata cggccgatgg ttgccagtaa ttcatcttga gtagcctgaa gtccgaggct
      421 caaacgattg aatccggcct cccgcaatcc ggataaggag gtttgtgtga gagtgccggg
      481 atttgcctct aacgttatct cgcgtggcgc atctccatta acctgaagaa ttgaactgag
      541 caacccggct aaaagcccgg tcggaagata gctgggggtt ccgccgccaa aataaaggga
      601 acccaaaggc tgggcgaagg gcatataccc ggccaactcc atgcggagtg cctttaagta
      661 cgcggggatc aattcatcgt atccgctgta tgaattgaag tcacaataaa ggcatttctg
      721 tttgcagaac ggaaaatgca aatataaacc aagcttctca ccgactattc acggtcacct
      781 cctccaaagg cccattcatt tggaaaccgg cactccggtc gaaagtttag gataatcaag
      841 ttgcattcat gaccttttaa cgcggttgcc agttgacgat aatgcctaat taagttattg
      901 taatggatag aagggtattt ggaatcattg ggttaaaatt cctcaactca agcgaacacg
      961 aagtgcccgt ggaaatatcc caaggcgatg ggggtccggt aaatggcttt ctgtctgggt
     1021 tctagattaa tagtgaacag tccgctcacc gaatacttgc gtgaggcggc gctgcagttc
     1081 aaagtcatcg agcttcaggc cgatcccagc tatttttcgg cgaattacgc gcttaccgtt
     1141 aatgaaaaaa aagtactccg gatctaccgg gagcgttacg ggtttcaact gaccatgcat
     1201 gccccgttta tcaatctgcg gttaggtgcg atcaatcttg aagaacgcca actggccatc
     1261 aatatcatga tcaacgccat gcaagccgcg tcggatctgg aaattcaact ggtaacattc
     1321 catccttgca cgctggagcc gggcgctccg gaaaaatacc atgagaactc tttattggaa
     1381 gagggcagta tcgccttatt attaaaagtt gccaaaaagc tgggcattac cctactaatg
     1441 gagaatatgc cgcttgtacc ggaatttcat tcgaaaacca ccgacggttc caggtttcaa
     1501 gaactgctct ggctcttccc tgagcctgag ttcggcctga cgatcgacat cggccatgcc
     1561 ctgcaggccg gaatcccacc ggaaggctta ttaaaaatgg atcggatccg tcattttcat
     1621 cttcacgaaa atgaccgcac catagataat catcagccga tcaccacaca cctcgagtgg
     1681 tgggaaaaac tattcaagac actaaccaaa aagttccccg atgcgtacgg aattttggaa
     1741 atgagtcaat ttacggatca aatcgcaagt ttgaaagaac tccaaccctt tttgggtaag
     1801 cgggccttgc cgaaacccgg caaaggtcag atgattccgc cgctcatcgt agattaattt
     1861 gtggttgcgg cggtcgtcct cacggaggct agggttaccc ttccctttga aaaaacaatc
     1921 tgaaccagct tcgcaggaaa gggcaagggt taggttcagg tctcatcgtc gatcttcagt
     1981 accgccaaaa atgcctcctg gggtacctcg acggaaccca actgtttcat ccttttcttt
     2041 ccttcctttt gcttctctaa caatttccgt tttctactga tgtcgccccc gtaacacttg
     2101 gccaatacat ctttccgcaa agcctttacg gtttctctgg caatcacctt ggtgccgata
     2161 gcagcctgaa tcgggacttc aaatagctgg cgagggatga tatccttcaa tttctccgcc
     2221 agttgcctgc cccgcgaata ggctttgtca cgatgaacga tagcggaaag ggcgtcgacc
     2281 accttctcgt tcaagagaat atccagtttg accagttccg actggcgatg tcccagatac
     2341 tcgtagtcta aaga
//
LOCUS       DASVMB010000204         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_319296,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000204 DASVMB010000000
VERSION     DASVMB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319296"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF531_06175"
     CDS             complement(<1..256)
                     /locus_tag="VF531_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7713589.1"
                     /translation="MGYHVQVEPGVKIYVEDLNPEGRKTILFLHGWPGDHRLFEYQFD
                     QLPKMGYRCIGVDTRGFGSSDKPWKGYDYDRLTDDIRCVIE"
     gene            complement(436..1206)
                     /locus_tag="VF531_06180"
     CDS             complement(436..1206)
                     /locus_tag="VF531_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7713590.1"
                     /translation="MSNERIQQILQLLEHKGEIQLQQLKTVFPDVSMMTLRRDLTSLE
                     QQGRLIRTHGGAVSIKKIASQGVRGEEDAYSRRASENTAAKVKIAEKAVTLVEKGRSI
                     YFDAGSTVMCLAEILPDENFSILTSGLNIALELVKKSQISVVSIGGLVNRNTLSASGP
                     NSLSMLDTINIDLAFMSASGFSLDSGFTVSNVYECELKRKIVHRAKNVIMLMDTSKIN
                     KNQPFTFAGLADIDHWICENQLPAEYVQAAIEHQVEVL"
     gene            complement(1365..>2972)
                     /locus_tag="VF531_06185"
     CDS             complement(1365..>2972)
                     /locus_tag="VF531_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7713591.1"
                     /translation="QFLARMGAGVNPEQMHSERVDGYNPLAVIDVIRRKKKLLEEKQG
                     PVLLDTLTYRFTGHSPSDSSSYRTKEEIEAWMRQDSLITYKEELVKAKVATEDDFTAI
                     WENTRQMITQILRLAVDDSISPRMDLARYPNTIGDLIFSNQRIEKMADRPCETLIPKA
                     ENPRLEQIQKKVRVGVEDGKPVAKNRVYQLRDGIFEAILDKFYTDPTLIAYGEDNRDW
                     GGAFAVYRGLTEALPYHRFFNAPISEAAIAGSAVGYALSGGRVIAELMYCDFLGRAGD
                     EIFNQLSKWQSMSAGMLKMPVVIRVSVGSKYGAQHSQDWTSLVTHIPGLKVVFPATPY
                     DAKGLMNSALMGTDPVIFFESQRIYDIGEMFHLEGVPEGYYEIPIGEPDIKKPGKDIT
                     ILSVGATLYRVMDAARILEEKYGLSAEIIDARSLVPFNYETVIASVKKTGRILLASDA
                     CERGSHLNDFARNISDLAFDYLDAPPVVVGARNWITPAHELEHFFFPQPEWIVDAIHE
                     KILPLDGHLPRTNFTGVEQLRLHRLGV"
BASE COUNT          703 a          768 c          743 g          758 t
ORIGIN      
        1 cttcgatcac gcagcggatg tcatccgtta accggtcata gtcgtagcct ttccaaggct
       61 tatcggaact gccgaatcct ctggtgtcca cgccaatgca gcgatatccc atcttgggaa
      121 gctgatcaaa ctggtattca aacaacctgt ggtctccggg ccaaccgtga agaaacaaga
      181 tggttttccg gccctccgga ttaaggtctt ccacatatat ctttactccc ggttcaactt
      241 gaacgtggta tcccaaatat gatgccccct ttagatgctc acttcactat aatgctatgc
      301 ggatgcgaat tgggtagaat cggtttctga caatcgtacc aaggggcata tattcttttg
      361 ggatgcaatg ggaacggtgg tgagatgatc gtgcgtctct gattcaacgc gttaattttg
      421 gtttggcgcc ttcacctaaa gaacctcaac ctgatgttca atcgccgcct gtacatattc
      481 ggccggtaat tgattttcac agatccagtg atcgatatca gccaggccgg caaaggtaaa
      541 gggctgattc ttattgattt tgctggtatc catcagcata atcacgttct tggcccgatg
      601 gacaatcttg cgtttcaatt cacattcata gacattggag actgtaaaac cgctgtcgag
      661 ggagaacccc gaggcggaca tgaaggccaa atcaatattg atggtatcca gcatggagag
      721 ggagttgggg ccggaggccg acaacgtatt ccggtttacc aggccgccga tggagaccac
      781 cgagatttgc gactttttta ccagctccag cgcaatgttc aaaccgctgg tcaggatgga
      841 aaagttctcg tccggcagga tctcggccag gcacatcacg gtggaaccgg catcgaaata
      901 gatggaacgg cctttttcaa ccagggtgac ggctttttcc gcgattttta ccttggctgc
      961 ggtgttctcc gaagcccgcc gggaataggc gtcttcttcc cctcttaccc cttgactggc
     1021 gatctttttg atgcttacag ccccgccatg ggtacggatt aagcggccct gttgctcaag
     1081 actggtcaaa tcccggcgta acgtcatcat ggatacatcc gggaagacgg ttttcaattg
     1141 ttggagctgg atttcacctt tatgttcaag gagctgcaag atctgctgta ttcgttcgtt
     1201 actcaccgga tcactctccg ccctttgctg cattttacca tataaatata agtatccgat
     1261 cataaccagt attaccatta tgatgggata cttatatttg tcagtaatac tttaaaccgt
     1321 gaaaaaagga atgtcaatca ctctaacggt atttttccaa tttgtcatac acccaaccgg
     1381 tgtaaacgca actgctccac gccggtaaag ttggtccggg gcaggtgtcc gtcaagcggc
     1441 agtatctttt cgtggatcgc atccacaatc cactccggtt gcgggaagaa gaaatgctcc
     1501 aactcatggg cgggggtgat ccaattgcgg gcgcccacca ccaccggcgg ggcatccagg
     1561 taatcaaagg ccagatcgct gatgttgcgg gcgaagtcat tcaaatggga accgcgttca
     1621 caggcatcgc tcgccaaaag aattctgccg gtcttcttta cggaggcaat gactgtttca
     1681 tagttgaaag gaaccagtga acgggcgtca atgatctccg ccgacaggcc gtatttttcc
     1741 tccagtatcc tggcggcatc cattacccgg tagagggtgg cgcccaccga aagaatggtg
     1801 atatccttgc cgggcttctt aatatcgggt tcgccgatgg gtatttcata ataaccctcg
     1861 ggaacgcctt ccagatgaaa catttcaccg atgtcgtata tacgttggct ttcaaagaaa
     1921 atcaccggat ctgttcccat gagggcgcta ttcattagcc ctttggcatc gtagggcgtt
     1981 gccggaaaga cgactttgag cccgggaatg tgcgtcacca gcgaagtcca atcctgggaa
     2041 tgctgggcgc catattttga accgacggaa acccttatca caaccggcat cttcaacatg
     2101 ccggcgctca tcgactgcca tttcgagagt tgattgaata tttcatcacc ggcgcggccc
     2161 agaaaatcgc aatacattaa ctcggcgatt acccggccgc cggatagggc ataacccacg
     2221 gcggaaccgg cgatggccgc ttccgaaatc ggcgcattga aaaagcggtg atagggaagg
     2281 gcctcggtca ggccgcggta aaccgcaaat gcgccgcccc aatcccggtt atcttcgccg
     2341 taagcgatta aggtgggatc ggtatagaac ttatccagaa ttgcttcaaa gataccgtcg
     2401 cgtaactgat aaactcgatt tttggcaacg ggtttgccgt cttcgacacc cacccggacc
     2461 ttcttttgaa tctgctctaa acgagggttt tcggctttgg gtatcagcgt ttcgcaaggc
     2521 cggtccgcca ttttttcaat tcgttgattg gaaaagatta agtccccgat ggtattggga
     2581 taccgcgcca gatccatccg gggtgaaatg ctgtcgtcaa cggctaatcg aagaatttga
     2641 gtgatcattt gacgggtatt ctcccagatg gcggtgaagt cgtcttcagt ggcaaccttg
     2701 gctttcacca attcctcctt atacgtaatc aacgagtcct gccgcatcca ggcttcaatc
     2761 tcttcttttg tgcggtaact ggaagaatcg gacggggaat ggccggtaaa ccggtaggtc
     2821 aatgtatcga gcaagaccgg gccctgtttc tcctccagca gttttttctt gcgccggatg
     2881 acatcgatca ccgccaatgg attataaccg tcgacccgct cggaatgcat ctgttccgga
     2941 ttgactcctg cacccatccg ggccaaaaac tg
//
LOCUS       DASVMB010000205         7043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_101781,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000205 DASVMB010000000
VERSION     DASVMB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101781"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2262)
                     /gene="dnaE"
                     /locus_tag="VF531_06190"
     CDS             complement(<1..2262)
                     /gene="dnaE"
                     /locus_tag="VF531_06190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7713592.1"
                     /translation="MAIPDKFVHLHVHTQYSLLDGAAAIDTLLAQAVNFGMPALAITD
                     HGVMYGCLKFYQQARKLGVKPILGSEVYVAPRGLRDKTPKLDENPFHLVLLAEDPTGY
                     KNLMKLVSIASIEGFYYKPRIDLDTLRLYHQGLIGLSACMSGEIPRLIMAGSFAAAKE
                     RIRLYQDILGKENFFLELQYQTLDGQRNLNRYLAQLASECGAPLVATNDVHYLNREDA
                     QVHDVLLCIQTGKTVSDPNRMKFATPEFYLKSAAEMGEIFADYPEALANTLRIAERCQ
                     VELELGKFHMPQYQLPEVKTPDGYLEELCRQGIEDRGLAWDDAYESRLRYELGIIGQM
                     GFSGYFLIVRDFVHFARENRIMVGPGRGSAAGSLVAFLLGITSLDPLEHGLLFERFLN
                     PERISMPDIDIDFCFERRGEVIAYVRRRYGADRVAQIITFGTMAAKAAVRDVGRALGI
                     AYGEVDRVAKLIPGALGITIADALQESAGLRELAAENPRISQLIGIASKVEGFPRHAS
                     THAAGVVISGEPLTDYLPLGLSNEDEIITQFPMEDIEALGLLKMDFLGLRTLTVLRDT
                     VALIEENRGIRVDIEGIPLDDPLTYRKLQEGDALGLFQLESSGIRRLLVRLKPERLDD
                     LTALVALYRPGPLGSGMVDDFIKARHGQKTITYLHPTLEPILSETYGVILYQEQVMRI
                     ASTLGGFSLGEADLVRRAMGKKKPEVLASLKEKFLKGADRNGIASDTAAAIFELMEYF
                     AGYGFNKSHSAAYA"
     gene            complement(2344..2808)
                     /locus_tag="VF531_06195"
     CDS             complement(2344..2808)
                     /locus_tag="VF531_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713593.1"
                     /translation="MVDSLRWRLLGLVDYKTVEIYADFKDNLIVIPSGATVDSVQELH
                     HPYNDAQLVALLTMALAKSTYHEGWDQRLENILEQYLGVAEPAEAIRGRRLVFFSWSR
                     RGGYSVTPTKALGGLNVYELPGISLGKRISGHMLVHAVKQALKTATPPRIHE"
     gene            complement(3047..4216)
                     /locus_tag="VF531_06200"
     CDS             complement(3047..4216)
                     /locus_tag="VF531_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713594.1"
                     /translation="METQTINPLPAPGFRNRLQLGAKSYVMVFALLIIWLLFFLLTQG
                     TFLSPRNLSNLARQMSITGILALGMVLIIVAGHIDLSAGAVVALTGAASAILQVRFGW
                     GLTEAVGIALLIGLAIGFWQGFWVCYLRVPAFIVTLGGMMIFRGVVLGVTEGRTISPL
                     PNGFVAIGQSFLPVEIGWVLAVIGMVYYVYARVKRRIDRRRYGFEVLPLILEVLVILG
                     VMGLIGLSVWVMNDYEGVPFPVLLLLGLALCFVVISRKTRFGRYLYAIGGNNEAARLS
                     GINIRLTTMFAFVLMGILCAIGGIVLTARLNGATTSAGTSFEMDAIASCVIGGTSLMG
                     GEGSVVGAIIGALVMASLDNGMVLLNTPTYWQYIVKGFILMAAVLVDFQMKRRGN"
     gene            complement(4218..5735)
                     /locus_tag="VF531_06205"
     CDS             complement(4218..5735)
                     /locus_tag="VF531_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter ATP-binding protein"
                     /protein_id="HEX7713595.1"
                     /translation="MSSPYILEMHQISKTFPGVKALDEVELKVFPGEVHALVGENGAG
                     KSTLIKVLSGVYPAGTYEGKIFISGKLCRFHNIQEAEAAGIAVIHQELTLIRELTVGE
                     NIFLGNEPLKRGMIDWDRLYFESRKLLSRVRLDLGAWRKIKELGLGQQQLVEIAKALS
                     KQARILILDEPTAALTEGETHNLMVILKELRAEGVTCIYISHRLGEVFEIADRVTVLR
                     DGCTVGSAPVGELNESKIIAMMVGRELTDLYPREHHQPGSILLEVEDLTVVDPTRPDH
                     RILNRISFQVRAGEILGIAGLMGSGRTELVMSLFGAYPGKVNGTVRLNGKPIRIRSPF
                     HGIQNGLGLVTEDRKRYGLVLKMNVMENLTMAGLRQVSGVFINPNEELKAANGFVGEL
                     RIKTPSLETQVNHLSGGNQQKVVVGKWLLTKPKILFLDEPTRGIDVGAKVELYRIMND
                     LVRQGVGVVMISSELPEILGMSDRILVMCQGRIAGELSGTTATQEQVMYFATGGR"
     gene            complement(5835..6836)
                     /locus_tag="VF531_06210"
     CDS             complement(5835..6836)
                     /locus_tag="VF531_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7713596.1"
                     /translation="MKKGFRILLITLILIAIAIAPSLGAKKMKIGLSLATLAEERWKV
                     DRDLFIAEANRLGADVIVAAANADENLQNSQVENLITQGVDALVVVAQNGETASALVN
                     SAHKSKIPVIAYDRMIKNCDLDLYVSFDSVKVGELMADYVCKLRPEGSYFVINGSPTD
                     NNAYLVRQGFTNILKKYPKIKVIFEQWCDSWSPEKALKFVENGLTQFSNKVDVVLCAN
                     DGTAGGAIQALAEQKLAGKVLVTGQDAELAACKRVVAGTQSVTIYKPIRKIAETAAKA
                     AVEMAQKHEAAGINAATDNGRIKVPSIMLEPIQVDKDNMRSVIIADKFHTESDIYGK"
BASE COUNT         1746 a         2029 c         1638 g         1630 t
ORIGIN      
        1 cgcatatgct gccgaatggg atttgttgaa accgtaaccc gcaaaatact ccatcaattc
       61 aaagatagcc gcggcggtat ccgatgcgat gccgttccga tccgcgcctt ttaaaaattt
      121 ttccttaagc gaggccagca cctcaggctt tttcttgccc atggcccggc ggaccagatc
      181 cgcctctcca agagagaaac cgcctaaggt acttgcgatc cgcatgactt gttcctggta
      241 tagaatgaca ccatatgtct cgcttaaaat cggctcaagc gttgggtgca ggtaggtaat
      301 ggttttttgg ccatgacggg ctttgatgaa gtcgtccacc attccgctac caagcggccc
      361 cggacgatag agcgccacaa gggcggtcag atcatcaagc ctctccggct ttaaccggac
      421 aagtaatctc cggatcccgc tgctctcaag ctggaacagg cctaaagcat ctccctcctg
      481 taatttacgg taagtcaggg gatcatccag cggtattccc tcaatatcca cccggattcc
      541 ccggttttct tcgatcaaag ccacggtatc acgtagcacg gtcaaggtac gtagccccag
      601 aaagtccatt ttaagcagac ctaatgcctc gatatcctcc atcgggaact gcgtgatgat
      661 ctcatcttcg ttagagaggc ccagcggtag ataatcggtg agcggttcac cggagatcac
      721 gacacctgcg gcgtgagtcg aggcatgacg gggaaacccc tccaccttag aggcgatccc
      781 aatcaattgc gagattctag gattctccgc cgccaactca cggagaccgg ccgattcctg
      841 cagggcgtcg gcaattgtaa taccaagagc gcccggaatg agctttgcaa cccggtccac
      901 ttcaccgtaa gcgatgccga gagcccgtcc cacatcccgg accgctgctt ttgccgccat
      961 ggttccgaag gtgatgatct gcgccaccct gtccgcgccg tagcgtcgcc ggacgtaagc
     1021 gatgacctcg ccgcggcgtt caaaacagaa atcgatatca atatccggca tcgagatccg
     1081 ttccggattc aagaagcgtt caaagagtaa accatgttcc agggggtcga gactggtgat
     1141 ccccagcaag aatgcgacca gactcccggc tgccgagccc cggcccggtc cgaccatgat
     1201 ccggttctcc cgggcgaaat ggacgaaatc ccggacgatt agaaagtaac cggaaaatcc
     1261 catttgccct atgattccca gttcataccg gagccgggat tcgtaggcat catcccaagc
     1321 caaaccccta tcttcgatcc cctgcctgca aagctcctca agatacccgt cgggggtctt
     1381 cacctcaggc aattgatact ggggcatatg aaacttgccc aactccagct ccacctgaca
     1441 gcgttccgcg atccggagag tgttggcgag tgcctccgga taatccgcga agatttcccc
     1501 catttccgcg gcggatttca gataaaactc gggagtggca aacttcatcc ggtttggatc
     1561 actcactgtt tttccggtct gaatgcataa gagaacgtcg tgaacctgtg cgtcttcccg
     1621 gttgaggtaa tgcacatcat tggtcgcgac aaggggggca ccgcattccg aggccaactg
     1681 ggccaaatac cggttcagat tcctttggcc gtcgagggtc tgatattgca actccaaaaa
     1741 aaagttctct ttgcccagga tatcctgata aagccggatc cgttccttgg ccgccgcgaa
     1801 agaaccggcc ataatcaacc ggggaatctc cccggacatg caggcgctta atccgatgag
     1861 tccctgatgg tatagacgga gcgtgtccag atcgatgcgc ggtttataat agaagccttc
     1921 aatgctggcg atggatacca gcttcatgag attcttatat ccggtcggat cttcggccaa
     1981 aagcaccaga tgaaacgggt tctcatcaag cttaggtgtc ttatccctga ggccgcgcgg
     2041 agcaacatag acttcgctgc caagaatggg tttgaccccg agtttccggg cttgctggta
     2101 aaatttaagg cagccgtaca tcaccccgtg atcggtgatg gctaacgccg gcattccgaa
     2161 attcacagcc tgtgcaagca gggtgtcaat cgcggcggcc ccatccagta aggagtattg
     2221 ggtatgtacg tggagatgaa cgaatttgtc aggtatggcc atggtttcac ccgtccggca
     2281 ctattttgag tctaaaacaa tttgaacccc aaataattgt gagggcgcgc gtatgcgcgc
     2341 gactcattcg tgaattcggg gcggtgtagc ggttttaagg gcttgtttca cggcatgaac
     2401 cagcatatgt ccggaaattc ttttacccaa ggaaattccg ggaagttcat aaacatttag
     2461 accgcctaaa gccttggtag gagttacgct atatccgccc cgccggctcc aactgaaaaa
     2521 gaccagtctc cggccgcgaa tagcctctgc aggttcggcg actcccaggt actgttcgag
     2581 gatattttcg agccgctgat cccatccctc atgataagtg gatttggcca aagccatagt
     2641 gaggagcgcc acgagttgag cgtcattata cgggtggtgt agttcctgaa cggaatcaac
     2701 cgtggctccg ctgggaatca cgatcaagtt gtctttgaag tcggcgtaga tctccacggt
     2761 tttatagtcc accagaccca aaagacgcca acgcaaagaa tccaccatat gccggtaacc
     2821 ttggcatacg caccgaaacc aaaggaatag atggggagtt cgacgcgact tcaatggaca
     2881 tcacccgcga ttgatcataa ctcatatttt cgattctgac aattcttaaa agcggtatga
     2941 aaacattaat tttatgcttc aaggatcgct tttagatcat aaaaagactg gaactccagt
     3001 cccagtctaa aaagtctaac cttctgactt gatgcggtca tcttcatcag ttgccgcgac
     3061 gcttcatttg aaaatcaacg agtacggcag ccatcaagat aaatcccttc acaatgtact
     3121 gccaatatgt gggcgtgttg agcaaaacca tcccattatc gaggctggcc atgaccaggg
     3181 caccgatgat cgcaccgacc acactccctt ccccgcccat caggctggtt cccccaatca
     3241 cacaagaagc aatggcatcc atctcgaaag atgtccccgc cgatgtggta gctccgttaa
     3301 gacgggctgt caacacgatg cctccgatgg cacagagtat acccatcaaa acaaaggcga
     3361 acatggtagt caggcggata ttgatcccgg atagcctggc agcctcatta ttcccgccga
     3421 tagcatagag ataacggccg aaacgggtct tccggctgat gaccacaaag caaagggcca
     3481 gacctaaaag gagtaaaacc ggaaagggaa ctccctcgta atcattcatc acccaaacac
     3541 tcaagccaat caatcccatg acccccaaga tcaccagtac ctccaggatt agcggcaaaa
     3601 cctcaaagcc atagcggcgt cgatctatac ggcgtttcac ccgggcataa acataataaa
     3661 ccattcctat gactgccagg acccaaccga tctcaaccgg tagaaacgat tggccgatcg
     3721 ctacaaaccc attgggcaac ggcgatatgg tccgcccttc cgtgacaccc agaacaacgc
     3781 cgcgaaagat catcatcccc cccaaggtga cgatgaacgc cggaactctc aaataacaga
     3841 cccagaaacc ctgccaaaac ccgattgcca gaccgattaa caacgctatc cccaccgctt
     3901 ccgttaaccc ccaaccgaac ctcacctgta aaatggcgga ggccgctccg gtcagggcta
     3961 ccaccgctcc agccgaaagg tcgatatgcc ccgccacgat gatcaagacc attccgagcg
     4021 ctaaaatccc ggtaatcgac atctgacggg ccagattgga aagattacgg ggggacaaaa
     4081 aagttccctg ggtcaggagg aaaaagagta accagataat gagcagtgca aagaccatta
     4141 cataggattt ggctcccaat tgcaaccggt tacggaatcc cggggccggc aacggattga
     4201 ttgtttgagt ttccatctta tcttcctcct gtggcaaagt acataacctg ttcctgggta
     4261 gctgtggtcc cggagagttc acccgcgatc cgtccctggc acatgacgag aatccgatcg
     4321 ctcattccca aaatctcggg cagctccgag gagatcatca ccacgcccac gccttggcga
     4381 accagatcgt tcatgatccg gtaaagctcc actttggctc cgacatcaat cccgcgcgtc
     4441 ggttcatcca gaaaaagtat tttaggtttg gtgaggagcc atttccccac tacaactttt
     4501 tgctgattac cgccgcttaa atgattgact tgagtctcaa gggacggagt cttaatccgt
     4561 aattccccga cgaaaccgtt tgcggccttc aattcttcat tggggttgat gaagactccc
     4621 gagacctggc gcaaaccggc catggtcaga ttctccatga cattcatctt caacaccagt
     4681 ccgtatcgtt tccgatcttc ggtgaccaag cccagtccat tttgaattcc atgaaaagga
     4741 gagcgaatcc ggatcggctt cccgttcagc cggactgttc cattgacttt ccccggataa
     4801 gctccaaaaa ggctcattac caattcggtc cgccccgatc ccataaggcc tgcaattccc
     4861 aatatctctc ccgcccggac ctgaaaggag atccggttta ggatccggtg atccggccgg
     4921 gtgggatcga caactgtgag atcctcaacc tcaagcagga tcgaacccgg ttgatggtgt
     4981 tcgcgggggt aaagatcggt taattcccgt ccgaccatca ttgcaatgat ctttgattcg
     5041 tttaattctc ccacgggcgc gctgccgaca gtacaaccat cacgcaagac ggttacccga
     5101 tccgcgattt caaaaacctc gcctaaacgg tgcgagatat agatacaagt aacgccctcg
     5161 gcgcgcaact cctttaaaat caccataaga ttatgcgtct cgccctcggt caacgccgcc
     5221 gtcggttcgt cgaggatcaa aatccgcgct tgtttgctga gggccttggc aatctccacc
     5281 aattgttgct gtcccaatcc caattccttt atctttcgcc aggcgccgag atccagccgt
     5341 actcttgaca aaagctttcg gctctcaaaa tagagccggt cccaatcaat catgcccctt
     5401 tttaacggct catttcctag aaaaatattt tcacccaccg tcaattccct tatcaaggtc
     5461 aattcctgat ggatgacggc aattccggcg gcttcggctt cctggatgtt atgaaatctg
     5521 cacagtttcc cggagatgaa gattttaccc tcataggtgc ccgccgggta tacaccactg
     5581 agcaccttga tcaaagtcga tttaccggcg ccgttctctc cgacgagggc atggacctct
     5641 ccgggaaaca cctttagttc gacttcatca agggccttga caccgggaaa tgttttgctg
     5701 atttgatgca tttcaagaat atagggggaa ctcatcgaag gcaccccttt cgccccataa
     5761 aaactcccgc ccccgagcgg aagcttcgca aatcaggagg cagtcgcatc actgcctcct
     5821 tccgaaatcc gggcttattt gccgtaaata tccgattcgg tatggaattt atccgcgatg
     5881 atcacagaac gcatgttatc cttatccacc tggattggct ccagcatgat cgaaggtact
     5941 ttgatccggc cattatcagt ggcggcgtta attccggctg cctcatgctt ttgggccatt
     6001 tccaccgctg ccttcgccgc tgtctcggca attttacgga tgggcttata aatcgtcacc
     6061 gactgagtac cggcaactac ccgtttgcaa gcggccaatt ccgcatcctg accggtgacc
     6121 aggacttttc cggccagctt ctgctctgcc agggcctgaa tcgccccgcc ggccgttccg
     6181 tcattggcgc agagcacaac atctacctta ttactaaatt gggtcaaacc attctctacg
     6241 aacttcaaag ctttctccgg cgaccagctg tcacaccatt gttcgaagat caccttgatt
     6301 ttgggatatt ttttcagaat attggtgaaa ccctgccgaa ccagataggc gttattatcc
     6361 gtcggagaac cgttgatcac gaaataactg ccttccggcc gcagcttaca tacataatcc
     6421 gccatcaact ctcccacttt gacgctgtca aaggagacat acagatccag atcgcaattt
     6481 ttgatcatac ggtcgtacgc gatgaccgga atcttggatt tatgagccga gttcaccaag
     6541 gcgctggcgg tctcgccgtt ctgggccact accaccaagg cgtcgacccc ttgggtaatc
     6601 aggttttcaa cttgactatt ctgtaaattt tcatccgcat ttgccgctgc aacgattacg
     6661 tcggccccga gccggttcgc ttccgcgata aaaaggtcgc gatccacttt ccaacgctct
     6721 tccgccaggg tggccaaaga tagcccgatc ttcattttct ttgcacccaa agacggcgca
     6781 atggcgatcg cgatgagaat caaagttatc aagagaatcc ggaacccttt tttcatattc
     6841 tcatacccct ttcaatgtct ttatggaatc gaaactccta tcccaccagc catcttctcc
     6901 ttgacgacac ctcctttgtt ccatcgaatc gctggaacag cctcctatat tatcaagaac
     6961 atagagaccg gttcctttgt tattttcaaa ctatgtttta cctaccgccg ccagataaac
     7021 ggcaaattcg gtatcgctgt cca
//
LOCUS       DASVMB010000206         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_615279,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000206 DASVMB010000000
VERSION     DASVMB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615279"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(558..824)
                     /locus_tag="VF531_06215"
     CDS             complement(558..824)
                     /locus_tag="VF531_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HEX7713597.1"
                     /translation="MEIKLINIGFGNIVSANRIIAIVSPESSPIKRIIQESRDKGMLI
                     DATYGRRTRAVIITDSDHVILSAVQPETVAHRLNSKDSVLDTEE"
     gene            complement(860..1174)
                     /gene="mihF"
                     /locus_tag="VF531_06220"
     CDS             complement(860..1174)
                     /gene="mihF"
                     /locus_tag="VF531_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016457581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX7713598.1"
                     /translation="MALPKLSLEEKKKALKKAQEVRSKRAKIRQSLKNGKTTIREVLA
                     NINDDVVAKMRVVYLLESLPRIGKVKTKKIMNDIGIDETRRIQGLGNRQKQALIDRLG
                     CN"
     gene            complement(1187..2065)
                     /locus_tag="VF531_06225"
     CDS             complement(1187..2065)
                     /locus_tag="VF531_06225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7713599.1"
                     /translation="MALSMTGYGRGTNTDSEYTVIADLKSVNHRYLELYFKLPRPYSF
                     LEDRLRREIASRISRGKLEITVQIDRVLPETVQVSLNKPLLDSYMKAIEEIKTRYQPE
                     GTIKVESLLNLPDVFSIAKPEEDQERLSGLAVAAITEALDSLIAMRRNEGNGLCQDIQ
                     GKLNYLGQLRISLLEWAPQIVLHYQERLTKRVQELTGGLELDPNRIANEVAIFADKSD
                     ITEELVRIDSHLQQFSGLLKLSEPTGRRLDFLIQELNREINTIGSKASDLKAAQIVIA
                     FKAELEKIREQIQNIE"
     gene            2389..3597
                     /locus_tag="VF531_06230"
     CDS             2389..3597
                     /locus_tag="VF531_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7713600.1"
                     /translation="MSSQSLTIKKFIPYLVVALFASLLTAGILWAVAPRLFQPANLQS
                     AQEPKVMAAAFKVPTGSNDAFGPDSFINASKRVSAAVVNIDTVSVVSTAPNIPDEFRD
                     FFPSDIFGGEQKQVQGAGSGFIIRSDGYILTNEHVVRDAQGLKITLTNGKKYNGKVVG
                     VDAQTDLAVVKIDARNLPVVEMGDSKNLVPGQWVIAIGNPYGLHGTVTAGIISALQRS
                     LEDPEQRGQLIQTDAAINPGNSGGPLLDLSGKVVGINEAIIANSQGLGFAIPINLAKT
                     VSADLIAKGRVQRPATPWLGVAIGEVTEQIASYYGLANTAGAIIQVYPNSPAARAGLN
                     DGDIIVQINRQKINTPDDVSKIVKGCKVGQVLDVMVYRDAKPKVFKVRLEERPQQTNQ
                     QRSGLNPFGG"
     gene            3687..>4146
                     /locus_tag="VF531_06235"
     CDS             3687..>4146
                     /locus_tag="VF531_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7713601.1"
                     /translation="MTATTAGAMTANRTVADQKVLFESLVKTYEKPLFQFAYRLCGNH
                     YEAQDLLQEGLYRAYKSFARFEAGTAFDRWLYQIIHNLYIDHYRKKKNRPLMTSIDEP
                     VPYLENEKPLDIADWSANPEDEAVRGELGQQIQDGLKELPTEYRAAVILCD"
BASE COUNT         1082 a          995 c          920 g         1149 t
ORIGIN      
        1 tgtgccccct tgtattcaat gcctgccctg ccatataatc attccaatcc ctaaccttca
       61 aattttgggg tgtgccccat atgaagttta acaaaaccac atccggataa caactgcctt
      121 tagcggaaat caggctgcaa accaggccgt aataaggcgt agggttaaaa tatgtggtaa
      181 aatctgatta tatgcaatct gggtatttac aaaccgacta atatcatatg caacaccggg
      241 atattttatc cgtgagctgg gatatgttta actaaaccct tatcatcggt atgtccggtc
      301 ctaatcttca agagctaaat aaagggggac ccttttttcg tataagtatc ataccagtta
      361 tttttggatt gatcaagtga tgattccggt tttacggatc ccaaatcgat tttgaaacct
      421 ttgtggccaa aatgaaaccg gaatcatttt tctgccgaaa tcggtcccac tgcaatcgta
      481 cattcaattg agatactcag atcaaagttg tgatcgattc cggtaaaaat gggtttttgg
      541 ccaatccggt tggcgactca ctcttcggta tccagcactg aatccttgga gttcaaccgg
      601 tgcgctacag tttcaggttg gacagcggag agaattacat ggtcgctatc ggtaatgatg
      661 accgcccggg tacgtctgcc atacgtggcg tcgatcaaca ttcctttatc tctgctctct
      721 tggatgatac gtttgatcgg cgatgattcc gggctgacaa tagcaataat ccgattggcg
      781 gaaacgatat tgccaaaacc gatattgata agcttaatct ccataattaa aaccagggcc
      841 cccttcccat tgatgcaaac taattgcagc caagtcgatc aattaacgcc tgtttttgac
      901 gattaccgag gccttggatg cgacgggtct catcaatacc gatatcattc atgatcttct
      961 tggttttcac tttaccgatc cgcggtaacg attctagcag ataaaccacc ctcatcttgg
     1021 caaccacatc atcgttaata tttgctaata cttcccggat agtggttttc ccgttcttta
     1081 aactttgccg aatctttgca cgtttgctcc gtacctcttg tgcttttttc agggcttttt
     1141 tcttttcttc cagtgaaagt ttgggtaatg ccattgatct cacctcctat tcgatgtttt
     1201 gaatttgttc ccgtattttt tccaattcgg ctttaaaggc gatgacaatc tgcgctgctt
     1261 tgaggtcgct cgctttggaa ccgatcgtat tgatctccct gtttaattcc tggatcaaaa
     1321 aatctaaccg ccttccggtc ggttcggaga gtttcaataa tcccgagaat tgctgcagat
     1381 ggctgtcaat ccggactaat tcctcggtaa tgtcggattt atctgcaaaa atcgcaactt
     1441 cattcgctat ccggttgggg tcaagttcca acccgccggt taattcctga acccttttgg
     1501 tgaggcgttc ctgatagtgg agaacaatct gcggcgccca ttcgagcagg gatatacgga
     1561 gttgcccgag ataatttaat tttccctgaa tatcttggca taaaccgttc ccctcgttcc
     1621 ggcgcatagc aatcaatgaa tccagggctt ccgtaatggc cgcgacagcc aatccactta
     1681 atcgttcctg atcttcctcg ggtttggcga ttgaaaaaac atccggtaag ttcaataagg
     1741 attcaacttt tatcgtgcct tccggttgat agcgggtttt gatctcctca atggccttca
     1801 tgtaagagtc gaggagcggc ttgtttaaag aaacttgaac tgtttcggga agcacccgat
     1861 cgatctgcac cgtgatctct agctttcccc tggagatccg actcgcgatt tcacgacgta
     1921 aacggtcctc caaaaagcta tatggtcttg gtagtttaaa atacaactcc aaatagcgat
     1981 gattgaccga ttttagatcc gcaattacgg tatattcaga atcggtattt gtaccacggc
     2041 cgtagcctgt catgcttagc gccaatgggt tacctcttta cttttcgagt atggatatcg
     2101 cgtattaaaa tgcccaaaaa tcgagatcaa acctgcttct atatttgaac ttagtgatat
     2161 attcaacttg ttgtggcaaa ttcctccttt tttccaggat gttaccgtta tttctgggaa
     2221 cttttttttt gatgcgccgt tttgacaatt atagaatcga gagtgtaccg taacagattt
     2281 ttaatgaagt ttgattcagt gaaccggagc tctacccgaa aagttattga atccaaaaac
     2341 cattggctac aaaagctata atacaaattt agcaaggagg tcctacttat gtcatctcaa
     2401 tccttaacga taaaaaaatt cattccgtat ttggtggtag ctttattcgc ttcccttctt
     2461 accgccggta tcttgtgggc ggtggcgccc cggttgttcc aaccggcgaa tcttcagtcc
     2521 gcacaggaac caaaagtgat ggctgctgct ttcaaggtcc ccaccggcag taatgatgct
     2581 tttggtcctg attcattcat caatgcaagc aaaagagtct cagccgcagt ggtgaacatc
     2641 gacaccgttt cggtggtttc tacggccccg aatattcccg atgagttccg tgattttttc
     2701 ccgtcggata tcttcggtgg cgaacagaaa caggttcaag gagccggttc cggattcatt
     2761 atccgttccg acggttatat tctcaccaat gaacatgtgg tccgcgatgc ccagggtctt
     2821 aaaattaccc tcaccaacgg aaaaaaatac aacggtaaag tggtgggagt cgacgcgcaa
     2881 accgatctgg cggtggtcaa gatcgatgcc cgaaacctcc cggtggtaga gatgggcgat
     2941 tcgaaaaatc tggttccggg ccaatgggta atcgccatcg gtaaccctta cggacttcac
     3001 ggtacggtaa ctgctggaat catcagtgct cttcaacgtt cactggagga cccggaacag
     3061 cgtggtcagc tcatccagac tgacgcggcc attaaccccg gaaacagcgg cggaccgctc
     3121 ttagacctat ccggcaaagt tgtcggaatt aatgaagcca tcatcgccaa ttcacaggga
     3181 ttgggattcg cgatccccat caatctggca aaaacagtct ccgctgatct tattgccaaa
     3241 ggccgggtac aacgacccgc aaccccgtgg cttggagtag ccatcggcga ggtaaccgag
     3301 cagattgctt cttattacgg tctggccaac acagccggag caattattca agtctatccc
     3361 aatagccctg ctgcccgcgc cggcctgaac gacggggata tcatcgtaca gatcaaccgg
     3421 caaaaaatta atacgccgga tgatgtcagc aagatcgtca agggatgtaa ggttggccag
     3481 gttttagacg ttatggttta tcgcgacgcc aaaccgaaag tattcaaggt tcgcctggaa
     3541 gaacggccgc aacagacgaa tcagcaacgt tcgggactta acccattcgg gggttgaacc
     3601 aaaacccacg aatccatggc tgtaagattt tagtaaccac agaaacttaa aaatctgata
     3661 caataggact taagaaggtg atactaatga ctgctacaac cgccggagca atgactgcca
     3721 atcgaactgt ggccgatcag aaagtccttt tcgaaagctt ggtcaaaact tacgaaaagc
     3781 cgttgtttca gtttgcctac cgtttatgcg gcaaccatta cgaggcccag gatctgctcc
     3841 aggaagggtt atatcgggcg tataaatctt ttgctcgttt tgaggccggc acagcctttg
     3901 atcgctggtt atatcagatc attcacaacc tttatatcga tcattatcgc aagaaaaaga
     3961 accgtccgct tatgacctcc atcgacgaac ccgtacctta tctggaaaat gaaaaaccgc
     4021 ttgatatcgc ggattggtcc gcgaatcccg aggatgaagc ggtacgggga gaattaggtc
     4081 aacagattca agacggttta aaggaactgc ccaccgaata ccgagcagca gttattctct
     4141 gtgata
//
LOCUS       DASVMB010000207         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_541929,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000207 DASVMB010000000
VERSION     DASVMB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541929"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VF531_06240"
     CDS             complement(<1..166)
                     /locus_tag="VF531_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7713602.1"
                     /translation="MATPAVKFIGATKQIRSKTIVDNLTMDINKGEVFGLLGPNGAGK
                     TTTIRMMVGLI"
     gene            complement(423..1169)
                     /locus_tag="VF531_06245"
     CDS             complement(423..1169)
                     /locus_tag="VF531_06245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7713603.1"
                     /translation="MVEVKGLPDDLPITDAAATFVPLIAAVILVYRKEKWGGVKRLLM
                     RAFDYRKIRKKVWYIPILLLMPFLYILTYFVMRAIGLPLLIEWNIPPQVPLVFMAFFI
                     AAVGEELGYMGYAVDPLLSRWSALKTGLVVGSIWAIWHFPSMIKMGQTPMLMVNGFIV
                     TVAFRILYIWLYNNTGKSVFGVILLHAIGNTGRSVFPGGRSNFELADAAVGYYFIAIT
                     VIIIIFLWGPKTLARFRYDFYAKNDKPKIS"
     gene            1833..2765
                     /locus_tag="VF531_06250"
     CDS             1833..2765
                     /locus_tag="VF531_06250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7713604.1"
                     /translation="MNHKNDLLLDFRIAVMEQKLDLYGIVIRQHGEIITGHRWRSDDD
                     VQLYSVSKTFTSMAVGIAMHEGYFKLSDKVVDFFPEYLPGQPDEKLLKMTVKDLLAMG
                     TGHSYCPIVHAFESGLEPTDWVKLFIDTPLAYEPGTHFEYNNSATYMVSAIIRRTTGI
                     NTRDYLMPRIFTPLRIFNPQWEVCPQGITIGAKGLFLTTEKLSRFGQLLLNCGQWEGN
                     QLIPADYIAEATRPQMDNSDFNPGWATVENHQGYGYQIWMNSYPNTYRLDGLYGQYVI
                     CFPEKDAVVTITAHQEGNQSDILKVVWNTLADRL"
     gene            complement(2825..3844)
                     /gene="yjfF"
                     /locus_tag="VF531_06255"
     CDS             complement(2825..3844)
                     /gene="yjfF"
                     /locus_tag="VF531_06255"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEX7713605.1"
                     /translation="MKGKHIINPKFMPLMATIGLFSLMFGVSSVTLPGFFSLQNFYYL
                     LIDNAYLIIMAVGMTFVIISGGIDLSVGSLLALTTMVTSSLVEKQLVNPMIVIPLVLL
                     MGTVFGLFQGYLIQTFNLAPFIVTLGGLYLARGLCYIVSIDTIPIVHPFFTAVSQFQI
                     NVIGNNFISVNVVIAVIMTIIGIFLAHYTKFGRTVYAIGGNEQSALLMGLPVAKTKML
                     IYTFSGFCTALSGIAFTFYMRNGYALHGKGMEMDAIAAVVIGGTLLTGGEGYVFGTLF
                     GVLIYGTIQSYINFNGTLNAWWTRIVIGLLVFVFCLMQRFFETGKITRRADQKSVRKT
                     ETAAN"
     gene            complement(3841..>4692)
                     /locus_tag="VF531_06260"
     CDS             complement(3841..>4692)
                     /locus_tag="VF531_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713606.1"
                     /translation="TLVIATKGIDISVGSVVAISCATVAFMIGGDLIIKNGVQVPFTK
                     FPLAIAILTAIVAATAAGMWNGMLVSRVGMQPIIATLILMVAGRGIAMMITKAQIITI
                     YYEPFSFIGNGYLFGLPFSLYIVSVLLLVTLLFVRKTAIGMFIESVGINPTATRFSGI
                     NAKNLIFWCYVFTGFCAGIAGVIYCSNVKSADGNNAGNLMELDAILAVVLGGTSLNGG
                     KFYLTGSIIGALIIRTLTTSIWALGVPPEIAEVVKAVVIFIVSVIQSERLRTVLGAMF
                     SRKELAA"
BASE COUNT         1440 a         1101 c          968 g         1183 t
ORIGIN      
        1 cgatcaaacc gaccatcatc ctgatggtag tagtcttgcc ggccccattg gggccgagca
       61 acccaaagac ttcacccttg ttgatatcca tcgtcaaatt gtcgacaatc gttttcgacc
      121 ggatttgctt tgtcgccccg ataaacttaa ccgccggtgt tgccattttc ctacctgccc
      181 ttcatcgaaa tcaagccaaa caatgagata gattcaagcc acaacagcaa aaagagtaaa
      241 gcatggcatt aataactcaa agcgcgttcg cagcggccgg gattccttaa tgaccatccg
      301 gccgaatata aaataacctt ggtatgccac tgagatactt cacacatttt agtggaccaa
      361 ggcggatttg tatatggtca gatcggtcta tttatttaaa ccaggctaat cagctggtaa
      421 cattaggaga ttttcggttt atcgttttta gcataaaaat catatctaaa tcgggctaag
      481 gtctttggac cccacaaaaa tatgatgatg ataaccgtaa tggctataaa ataatagcct
      541 acagcagcat cggccaactc gaaattacta cggccgccgg ggaataccga cctgccggta
      601 ttgccgatgg catgaagcaa gataaccccg aagacactct ttccggtgtt gttataaagc
      661 caaatataga gaatccgaaa agcaaccgta acgataaatc cgttgaccat taacattgga
      721 gtctggccca ttttaatcat tgatggaaag tgccatatgg cccagattga tcctacaacg
      781 agaccggtct taagggcact ccatcgggat agcaggggat cgacggcata tcccatatag
      841 cccagctctt cacctacagc ggcaatgaaa aacgccataa aaaccaaggg tacctgagga
      901 ggaatattcc attcaatcag gagcggtagc ccgattgcgc gcatcacaaa gtaagtcagt
      961 atgtacaaaa agggcatcag caaaaggatg ggtatatacc aaaccttttt ccttattttc
     1021 cgatagtcga aggctctcat aagcagcctc ttgactccgc cccatttttc tttccggtag
     1081 acgaggatca cggctgcaat taaaggaaca aaagtggctg ccgcatcggt tatgggaaga
     1141 tcatccggaa gtcccttgac ttcgaccatt gttccaatga tccataaagg aattgaaaga
     1201 gcatagacca tcagaaaaaa ttttaatggg tatcgattga tagacccttc attcatgtaa
     1261 caatcccact ttcatgcctt ttgttctaaa agtcaaggac tacacggttg tctttttgaa
     1321 acaaaatgta ccaaaacacc ggttaaaaca tttaccaaaa tttctgccat atatttctag
     1381 aatttaaaat caaactcctt ctgaggtcaa tgatatggct aatggatttt aaccctatca
     1441 ctctccgggc tctagcaacc gtaccggatg gtaaacaaca aaaaatattt gacttttatt
     1501 gatgttgagc gggatatatc cggtggcggc aattagcaaa acatatattt tatggccgtc
     1561 cagatattga ccgggtaaca ggccttatcg tgcgggttgt tgataatgat taaaaatagt
     1621 aacaggaatt ggaaatcaaa tatggaaata gttatttggc attatataac ccaatttacc
     1681 gcactaatga tgaggcaaat gaatctgcag catcattggc tgaatcaaga aagaaagatg
     1741 aacggcaatc attcttgaca ctacctggaa ccataagtta aaattcggaa tcaacgttct
     1801 ttgaaatatt aaatactttt ggagttgata tcatgaatca caaaaacgat ctcttactgg
     1861 acttcagaat cgcagtcatg gaacaaaaac tggatttata cggaatcgtg atcagacaac
     1921 atggtgaaat cattaccgga catcggtggc gttccgatga tgatgttcaa ctatattcgg
     1981 taagcaaaac ctttacctcg atggccgtag gcattgccat gcatgagggc tattttaaac
     2041 tctccgataa agtggtggat ttctttccgg aatacctgcc ggggcagccg gatgaaaagt
     2101 tgctaaaaat gaccgtcaaa gacctgctgg cgatggggac cggccactca tactgcccga
     2161 tagtgcacgc ctttgagtcg ggtttggaac ctaccgattg ggttaagctg tttatcgata
     2221 ccccgcttgc ttatgaaccg gggactcatt ttgaatacaa caactccgcc acctatatgg
     2281 tgagtgccat cattcgccgc accaccggca taaatacccg ggattacctg atgcccagga
     2341 tatttacacc gctccgcatc tttaatcccc aatgggaggt ttgccctcaa ggtatcacta
     2401 taggagccaa gggattattt ctgaccaccg aaaaattgtc ccggtttggt cagctgttac
     2461 tgaactgcgg tcaatgggaa gggaaccaac tgattcccgc cgactatatc gcggaagcga
     2521 ccagaccgca gatggataac tccgatttta atcccggctg ggctacagta gaaaaccatc
     2581 aaggttacgg gtatcaaatc tggatgaact cctatcccaa tacttatcgc ctggatggac
     2641 tttatggcca atacgtgatt tgtttcccgg aaaaagatgc cgtagtgacg attacggcgc
     2701 atcaggaggg caatcagtcg gatattttaa aagtagtatg gaatacttta gcggatcgac
     2761 tttagtttga cgttgacttc acccttcaca cttgaataat tcataatcaa actccggccg
     2821 tcacttaatt tgccgcagtt tctgtttttc tcacggactt ttgatctgct ctccgagtga
     2881 ttttacctgt ttcaaaaaat ctttgcatca aacaaaatac gaaaactaaa agcccgatga
     2941 caattctggt ccaccaggca ttaagcgttc cattaaagtt gatataactt tggatggtgc
     3001 catagatcaa aacgccaaat agtgtcccaa aaacataccc ttctcccccg gttaaaaggg
     3061 tgcccccgat aaccaccgcg gcgatggcat ccatctccat ccctttgcca tgaagagcat
     3121 acccgttccg catataaaaa gtaaaggcaa ttcccgacaa ggccgtacaa aagccgctga
     3181 aggtataaat cagcattttg gttttggcta cgggcaaacc cattaacaat gccgattgct
     3241 catttcctcc aatcgcataa acagttctgc cgaatttggt ataatgagcc aggaatattc
     3301 cgataattgt catgataact gcgataacta cgttaacgga gataaaatta ttgccgataa
     3361 cgtttatttg aaactgcgat acagccgtaa aaaacgggtg gacaatcgga atcgtatcga
     3421 tgctgacaat gtagcataaa cctctggcca ggtataaacc acccaaagta acaataaaag
     3481 gcgccagatt aaaagtttga attagataac cctgaaaaag gccgaataca gtccccatca
     3541 aaagcaccag cggtatgacg atcatcggat tgacaagctg tttttccacg agactggagg
     3601 tcaccatcgt ggttaaggcc agtaaagacc ccacggaaag atcaatcccg cccgaaatga
     3661 tcacaaaggt cattccgacc gccatgataa tgagataagc gttatcaatc aataaatagt
     3721 aaaaattctg taatgaaaaa aaaccgggta aagtaaccga actgacaccg aacattaaac
     3781 taaacaaacc gatagtcgcc attaatggca taaatttagg attgatgata tgtttgcctt
     3841 tcatgccgcc agctccttcc tactaaacat tgcgccaagt accgtccgca gcctttccga
     3901 ttggatcacg ctgacaataa agattacgac tgcttttaca acttccgcaa tctccggcgg
     3961 cactcccaat gcccagatgg aagtggtcag ggtccggata atcagcgcgc ctataatgct
     4021 gcccgtcagg taaaacttcc cgccgtttaa ggatgttccg cctaaaacta ccgccaggat
     4081 cgcatccaac tccattaaat tgccggcgtt attcccatcg gcgctcttta cattcgaaca
     4141 ataaatgact ccggctatcc cggcgcagaa cccggtaaat acataacacc agaaaataag
     4201 atttttggcg tttattccgg aaaaacgggt cgcggtcggg ttgatcccta ccgactcaat
     4261 aaacatgccg atggctgttt ttcgcacgaa taataaggtt accagtaata aaaccgatac
     4321 gatgtacagc gagaacggca aaccgaataa atagccatta ccgatgaaag aaaacggttc
     4381 ataatagatc gtaataatct gggctttggt gatcatcatc gcgatcccgc gtccggcaac
     4441 cattaagatc aaggttgcaa taatcggctg catgcccact ctggaaacca gcattccatt
     4501 ccacattccc gctgcggtag ctgctacaat cgcagttaat atggcaatag ccagaggaaa
     4561 ctttgtaaag ggtacctgta ctccgttttt gataatcaga tcgcctccga tcatgaacgc
     4621 tacagtagca caggaaattg ccacaactga accaacagag atatctatcc ctttggtggc
     4681 aattactaat gt
//
LOCUS       DASVMB010000208         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_25824,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000208 DASVMB010000000
VERSION     DASVMB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25824"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VF531_06265"
     CDS             <1..769
                     /locus_tag="VF531_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7713607.1"
                     /translation="DEKKLAKIDFKNIPNYRLIGAAYKDLGFDPDNEYSVPYMWGTVG
                     ILYNKKMVRGTPDSWKILWDKKYAKQLLMLDSQRDSIGITLKMLGLSLNSKNPDELRK
                     AKEALIAQKPLVLAYVVDEVKDKMIAEEAALAVVWSGDAVYCIRENPDLAYFIPKEGS
                     NLWFDSMVIPEDAPHKEMAEKFINFMCDTQTAYRNAEYIGYASPQTGVIKKLPADLTQ
                     NRAFYPQAGDLANSEVFVDLGSMMKDFDKVWTEVKAE"
     gene            1122..1565
                     /locus_tag="VF531_06270"
     CDS             1122..1565
                     /locus_tag="VF531_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX7713608.1"
                     /translation="MHRPLYNEKTLKSRKLMTNGYSLPYNPDLVPRAVEMRNNMTPAE
                     FKLWTGFLTNFDFKVYRQRPIDNFIVDFYCSKLNLVIEIDGSVHDSLEAKVSDQERTE
                     ILQNYGLKVLRFSNNQVEEDFEWVCQKIKEQIPNPLRCLYPSPLK"
     gene            1797..3401
                     /locus_tag="VF531_06275"
     CDS             1797..3401
                     /locus_tag="VF531_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX7713609.1"
                     /translation="MWWQDGVIYQIYPRSFKDSNHDGIGDLKGIIAKLDYLNNGTPDS
                     LGVDGLWISPFFRSPMKDFGYDISDYRDIDPLFGTLDDFKELLAETHRRNIHIIVDLV
                     LNHTSDQHPWFAESQQDRTNPKVDWYIWHPGKNGKPPNNWFSEFELQNAWWYHPKRKE
                     FYLGTFTRNQPELNWRNPEVRKEMFDLVRYWLDMGVDGFRLDVINWFIKDDRFRSNPY
                     TLKSVDFQKHLYDRNRPESIDICRELRSITDQYQERMLVGEVYTDSSAIASSYCGNGN
                     DALHMAFNFNFLFQPWDARKIYHSVIDWYDHLAPGAWPNFTFSNHDNLRHIYRYRKGR
                     WTNARARVAATLLMTLRGTPFIYYGEEIGITGSNLHRKDAQDPLTKKAWPIKKFRRDM
                     ARTPMQWDASANAGFSAAAPWLPVDADYLTNNVAVQSGQPGSLLNFYKQLIWLRKEQP
                     TLQSGEIKFLPEYLPSLLVYRRENKSGSILVILNFSGKAVQLEQLPDKSESLRVLFGT
                     HRKKGSTLMSSERPVISAYEVIVAMQ"
BASE COUNT          935 a          831 c          825 g          813 t
ORIGIN      
        1 cgacgaaaag aaactggcaa agatcgactt taaaaacatc cccaattacc ggttgatcgg
       61 tgcggcctat aaagacctgg gctttgatcc ggataatgag tactccgtcc cttatatgtg
      121 gggtacggtc ggcatccttt ataacaaaaa aatggtccgc gggactccgg acagctggaa
      181 gatcctctgg gacaagaagt acgccaaaca gcttctgatg cttgacagtc aacgcgattc
      241 catcggcatc accttgaaaa tgttggggct ctcgctaaac agcaagaacc cggatgaact
      301 gcggaaggcc aaggaagcct tgattgccca aaagcctttg gtcctggcct atgtggttga
      361 tgaggtcaag gataagatga ttgccgaaga ggcggccctg gcagtggtct ggtccggtga
      421 cgcagtttac tgcatccgcg aaaatcccga cctggcctac tttattccca aggaaggctc
      481 caacctctgg ttcgacagca tggtgatccc ggaagacgcg ccccataagg aaatggctga
      541 gaagttcatc aacttcatgt gcgacaccca gaccgcatac cggaatgcgg aatacatcgg
      601 atacgcctcg cctcagaccg gggtcatcaa gaaactcccc gccgatctga cccaaaatcg
      661 cgccttctat ccacaagccg gggatctggc gaacagcgaa gtattcgtcg atctgggcag
      721 catgatgaag gacttcgata aagtatggac tgaagtgaag gcggagtaag gtccaaatca
      781 tttattcaaa aaaccgaggc gcaataaaat gcgcctcggt tttttgttgg ctgctcccct
      841 agtccattag ggtagatggc ctatcatctt gccatgcttc gatgtcttta ccctagccct
      901 taaaggggat ctgcttgatt ctcaagcctt tagttggact tcgcagttta atcccggcct
      961 attgacccaa gattcttgcc tgttcccttt aaggggaagg gtgataagac tattattgac
     1021 atataaaaat gtgtgctaaa aaggatatat tttacaactg aagaatttct gaaatactaa
     1081 cccgttttta acaatttcta aaaaacactt aggaggtgtc aatgcacaga cccctttaca
     1141 atgaaaaaac acttaaaagc cgtaaactaa tgaccaatgg ctactctctg ccatataatc
     1201 cagatttggt tccaagagca gtagaaatga gaaataatat gactccggcc gaatttaaat
     1261 tatggacagg gtttcttact aattttgatt tcaaagttta tcgccaacgg ccaatcgata
     1321 attttattgt agatttctat tgctccaaac taaacttagt aattgagatt gacggttctg
     1381 tccacgattc attggaagca aaagttagtg atcaagagag aactgaaata ttacaaaatt
     1441 atggtttaaa agtactccgc ttcagtaaca accaggtaga agaagacttt gaatgggtgt
     1501 gccaaaaaat caaagagcaa ataccgaacc cgcttcgctg cctttaccct agtccgttaa
     1561 agtagagttg tattacaact cttaagaaaa cggtgtcacc attttcaggg gattagcttg
     1621 attctcaagc ttttgtcggt tggacttcgc aggttagcct cagcctattg acccaagatt
     1681 ctagcaagtt ccctttaagg gaaagggtaa tgggccctta ccaacatgta acagcagtga
     1741 tacaatagaa ttgttattat tattggaaaa gattcaatta aaggttgtga tcatcaatgt
     1801 ggtggcaaga cggagtaatc tatcaaattt acccgcgaag cttcaaagac tcgaatcatg
     1861 acggcatcgg ggacttaaaa ggaatcatcg ccaagctcga ctatctcaat aacggaaccc
     1921 ctgattccct tggggtggac ggtttatgga tatcgccgtt tttccgttcc cccatgaagg
     1981 actttggtta cgatatctcc gactaccggg atattgaccc gctcttcggc accctggacg
     2041 atttcaagga gctcctggcg gagacgcatc gccggaatat ccacatcatc gtcgatctag
     2101 tactcaatca cacctccgat cagcatccct ggttcgccga gtcacagcag gaccggacga
     2161 acccgaaagt agactggtat atctggcatc ccggtaaaaa cgggaaaccc ccgaacaact
     2221 ggttctccga gtttgaactg caaaacgcct ggtggtatca tcccaagcga aaagaatttt
     2281 acctgggcac ctttacccgg aaccagccgg aattgaactg gcgcaatccc gaagtgagaa
     2341 aggaaatgtt cgacttagtc cggtactggc tggacatggg ggtggacggg ttccggctcg
     2401 atgtgatcaa ttggttcatc aaggacgacc ggtttcgcag caatccctat accctgaaaa
     2461 gcgtggattt ccaaaaacat ctctatgacc ggaaccggcc ggaaagcatc gatatctgcc
     2521 gggagctccg gagtatcact gatcaatatc aggaaagaat gttagtcggc gaggtctata
     2581 ccgacagctc cgccatcgct tccagttatt gcggaaacgg caacgacgcg ctccacatgg
     2641 ccttcaattt caattttctc ttccagccgt gggacgcaag gaagatctac cattccgtca
     2701 tcgactggta tgatcatctc gccccgggag cctggcccaa tttcacattc agcaaccacg
     2761 acaacctgcg ccatatttac cgctaccgta aaggccgatg gaccaatgcc cgggcccggg
     2821 tggccgccac gctgctcatg accttgcggg gtaccccctt catctattat ggtgaagaga
     2881 tcggcatcac cggcagtaac cttcatagga aagatgctca agacccgctg actaaaaaag
     2941 cctggcccat caagaagttc cggcgcgata tggcgcggac cccgatgcag tgggacgctt
     3001 ccgccaatgc cggattctct gcagccgcgc cctggctgcc ggtggatgcc gactacctga
     3061 ctaataatgt ggcagttcag tccggacagc ccggttcgtt gttgaatttc tacaagcaac
     3121 tgatctggct tcgcaaagaa cagccgacac tgcaatcggg cgagatcaag ttcttgccgg
     3181 agtatcttcc gtcgctattg gtctatcgta gggagaacaa atccggatcg atactggtaa
     3241 tattgaattt ctcggggaaa gcggtgcaac tggagcagtt gccggacaag agtgaaagct
     3301 tacgggtact gttcgggact caccgcaaga aggggagcac cttgatgagt tcggagagac
     3361 cggtgattag cgcgtatgag gtgatcgtgg cgatgcagta aaga
//
LOCUS       DASVMB010000209         7358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_393748,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000209 DASVMB010000000
VERSION     DASVMB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393748"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1095)
                     /locus_tag="VF531_06280"
     CDS             complement(10..1095)
                     /locus_tag="VF531_06280"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX7713610.1"
                     /translation="MSDQSFSNGSRNSSDMAGASKKTFNEDDILLRVRDLKKHFLIQK
                     GLLRKTVGSVKAVDGVSFDVHTGETMGLVGESGCGKTTTGRCILQLEAPTGGEILFKK
                     DNALIPVNRHSIGSLRRDMQIIFQDPYSSLNPRMRIEQILAEPLETHGMNNKKEVRER
                     VADLLNAVGLASDLMHRFPHEFSGGQRQRIGIARALALNPKLIVCDEPVSALDVSIQA
                     QVINLLEDLQDQFGLTYIFVAHDLSVVKYICDRIAVMYLGEIVELTEVNELFVNPKHP
                     YTEALFSAIPIPDPDIEYGQIILEGDVPSPSDPPKGCHFNPRCRYAQDICRNEHPALG
                     PVGDPGHLVACHFAEKLGLRGVAQASA"
     gene            complement(1088..2161)
                     /locus_tag="VF531_06285"
     CDS             complement(1088..2161)
                     /locus_tag="VF531_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713611.1"
                     /translation="MAFFQSSSPLREEASAMNGVLTKELPEMGPDSLLHIEDLHTYFT
                     TEEGVVRAVDGVTLGIRRQKVLGVVGESGCGKSITALSAMQLIPRPKGKIHSGHIFYN
                     RDDKGVLDLAAQDPLGPVMRSIRGNEIAMIFQEPMTSLNPVFTVGDQIVEAVQLHRNI
                     PKNEAWDVAVDMISKVGIPNPAERAKEYPHCLSGGMRQRAMIAMALSCNPRLLIADEP
                     TTALDVTIQAQILDLMQRLQDEYQMAIMIITHNLGVVTRICDEVAVMYMGKVVEQGTV
                     RQIIKETLHPYTTGLINSIPDIGVRTQRRLNPIKGTVPDPFKLPKGCSFQPRCDRCFD
                     KCVTDPPLFEKAGSHKVRCWLYE"
     gene            complement(2700..4661)
                     /gene="htpG"
                     /locus_tag="VF531_06290"
     CDS             complement(2700..4661)
                     /gene="htpG"
                     /locus_tag="VF531_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX7713612.1"
                     /translation="MDQTSTKITKEFQAETKQLLDLMVHSIYTNREIFLRELISNASD
                     AIDKLHFQSLTNLDLLEGDSDFAIWLEIDEANHTLSITDNGLGMNYDEVIEDIGTIAK
                     SGTKAFIEKLQSQEGSQNPELIGQFGVGFYSAFMVSDKVTLLTKTPGANGVRWESTGD
                     GTYTIEPAEHSKRGTTVILTLNEAYRNPEKPEENFLNRYTLQNLVKKYSDYIRYPIKL
                     DFPKEERPKDKDGKEIEGAAPTITIETRTLNSMTPLWARNKSEITQEEYQQFYKNLFH
                     DWNEAAEVIHTKVEGAVEYTALLFIPSTAPFDFYSKEYRGGTKLYSKHVFVMDNCEEL
                     LPDYLRFVRGLVDSPDFSLNISREILQQSQQLKLIGKNLEKTVLKTLESMLAKERPKY
                     ETFWKEFGSALKAGVYLDFQSHERLQDLLLFPSSNAIEGYTTLSEYLKRMPENQKEIY
                     YITGKDRAAVEKLPQLESLRDKGFEVLYLFDRVDEFTIDSLHEYQKQPFKSVSRGDLA
                     LEGDEAAKEAKAKLEELTQKHEPMLKAIKEQLKDKVAEVRLTNRLKSSPVCLVSGDSG
                     ISLSMEQILAEMNQSMFKATRILEINPDHEVFNILERMYQADPQSNTFKEYNELLYGQ
                     SLLMEGITLENPVEFAAKIAELMIKAGKQ"
     gene            5178..6377
                     /locus_tag="VF531_06295"
     CDS             5178..6377
                     /locus_tag="VF531_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7713613.1"
                     /translation="MRFIGPGFLVTIGFIDPGNWATNVAAGSEFGYALLWVITLATGM
                     LIILQHNAAHLGIVTGYCLSEAATFYLAPWLSRAILVTAMFAAGATALAEILGSGIAL
                     NMLFGLPTKLGALLSAALVFWLLENNSYRKLEKLIIGFVSLIGISFLIELGYVHIHWT
                     EAMVSWVKPSLPSNSLLAVMSLLGAVVMPHNMFLHSEIIQSRQCNLENDAAKTRRLKY
                     EFLDTSFAMVIGWAINSAMILVAASTFFSQGIRVTDLSQAEQMLRPLLGNTAAVIFAL
                     ALLCSGISAAITAGISGGTIWAGIYGRPYDVKDRSSRLGAALTIIPAALVILLIRDTF
                     QGLIYSQLALGIQLPFTIFLQIYMTSSRRLMGKFVNSRLDKILLWTIGLVVTGLNIAL
                     LVMTVKP"
     gene            complement(6306..>7358)
                     /locus_tag="VF531_06300"
     CDS             complement(6306..>7358)
                     /locus_tag="VF531_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019453757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7713614.1"
                     /translation="IRKRAWKKGASLLSEVKSIHSKSTKGYLVALASAAVLSTTAILI
                     RHLTQSYHLPPLVLAFWRDGLVFFILLPVLGLFNPVLLRLDRSRLFYLGGFGLVLASF
                     NALWTLSVALNGAAVSTVLAYSSTGFTLLLGWCFLKERLNSFKILAVAFSLGGCAMVA
                     GALNPAAWNVNLTGIVTGILSGLCYALYTLMGRSAARRGLNPWTVLLYSFGFAALVLF
                     LCNLLPVRIFPGATGLKSIFYLKENLSGWGVLFLLAAGPTVIGFGLYNISLGYLPAGT
                     VNLIAALEPVFTAITAFSLLGERLSGMQLCGGLMILAGVLLLWKNEGNSTASGFHRHY
                     QQCDIQAGHHQSDGPE"
BASE COUNT         1813 a         1761 c         1881 g         1903 t
ORIGIN      
        1 gttcaatgtt caagcggatg cctgagcaac tccgcgaagt ccaagtttct cggcaaaatg
       61 acaggcaacc aggtgtcccg ggtctcccac cggaccaagg gccggatgtt cgttcctgca
      121 aatatcctgg gcataacggc aacgcgggtt gaaatggcat cccttgggag gatccgaagg
      181 gctgggaaca tcaccctcca agatgatctg gccgtattca atatccggat ccggaatcgg
      241 aatggcggaa aagagcgcct cggtatacgg atgtttggga ttgacaaaga gctcgttcac
      301 ctcggtgagc tcgacaatct cacccagata catcacggcg atccgatcgc agatatattt
      361 aacaacactg agatcgtggg cgacgaagat ataggtcaga ccgaattggt cctgcagatc
      421 ttcgagcaag ttgatgacct gggcttgaat cgatacatcc agagccgaaa ccggttcgtc
      481 gcagacgatg agcttggggt ttaaggccag agcccgggcg attccaatcc gttgtctttg
      541 accgccgctg aactcgtgcg ggaaacggtg catcagatca gaggcaaggc cgacagcatt
      601 taagagatcc gcgacccgtt cacggacctc ttttttgtta ttcataccgt gggtctccaa
      661 gggttccgcc aggatctgtt cgatccgcat ccgggggttg agcgaggagt acggatcctg
      721 aaagatgatc tgcatgtcac ggcggagtga accgatggag tgacggttca ccggaatcaa
      781 ggcattatcc tttttaaaca ggatctcgcc gccggtggga gcctcgagtt gcaaaatgca
      841 acgtccggta gtcgtcttgc cgcaaccgct ctcaccgact aatcccatgg tttcaccggt
      901 gtgaacatcg aagctgacgc cgtcgaccgc tttaaccgaa ccgacggtct tgcggagcaa
      961 acctttttga atcaggaaat gttttttcag atcccgcacc cgcaataaga tatcatcttc
     1021 attgaaagtt tttttagatg ctcccgccat atccgaactg ttccgggagc cattagaaaa
     1081 tgattgatca ctcatagagc cagcacctca ccttatggga tccggccttt tcaaacaggg
     1141 ggggatccgt aacgcattta tcaaagcagc gatcgcaacg gggttggaaa ctgcagccct
     1201 ttggcagttt gaacggatcg ggcactgtgc ccttgatcgg gtttaacctt cgctgggtgc
     1261 gtacaccgat atcgggaatc gagttaatca gtccggtggt gtagggatga agggtttcct
     1321 tgataatctg ccggactgta ccctgctcga cgactttgcc catgtacatg acggccactt
     1381 cgtcgcagat ccgggtgacg acccccagat tatgagtgat aatcatgatc gccatctgat
     1441 actcatcctg caatcgttgc atcagatcga gaatctgggc ctggatggtc acatccaaag
     1501 cggtggtggg ctcatcggcg atgagcagcc ggggattgca ggagagggcc atggcgatca
     1561 tcgcccgttg ccgcattccg ccgctgaggc aatgaggata ttccttggcc cgttcggccg
     1621 ggttggggat gcctactttt gaaatcatgt ctaccgcaac atcccaggct tcattctttg
     1681 gaatattgcg gtggagttgg accgcttcga caatctgatc cccgactgta aagaccgggt
     1741 ttaaggatgt catgggttcc tgaaagatca tcgcgatttc gttcccccgg atgctgcgca
     1801 ttaccggccc taaaggatct tgggccgcaa gatcaagaac ccccttgtca tcgcggttgt
     1861 aaaagatatg accggaatgg atcttccctt tggggcgggg gatcaactgc atagcggata
     1921 aagcggtgat actcttgcca caaccgcttt cacccacgac tcccagtact ttttgacgtc
     1981 gaatccccaa agtgacgcca tcaactgcac ggaccacacc ctcttcggtt gtgaagtaag
     2041 tatgcagatc ctcgatgtgc agcaacgaat ccgggcccat ctccgggagc tcttttgtca
     2101 aaacaccatt catggccgat gcttcttccc tcagtggcga actggattga aaaaaagcca
     2161 ttgaaataaa acctccggtt gaaatgaatt tgtctttcca aaagaatgta tgttgtataa
     2221 atttgaaaat aaaattattt caaatctatt ggacaaaaaa aggctaaatc ctcttcaaga
     2281 accagcgaat ttttataaat ttttgtaacc ttttccacac ctagaaatgg attttcattt
     2341 ggcgatttaa ctgtcggcaa aggagaatat ttgaatcaaa tatccatgga aaagaacacg
     2401 aatgcataag tatacataaa taccgccggt taatattcct caatgatccc gcgataatca
     2461 ggggtattga attgattagt atggctaaga aattagtttg agatataata agatattccg
     2521 atatctcttg aattaatcat gtcgcttaaa ataatcacct ggaaaaccaa tgaccatttc
     2581 tttttacaga cagaggggaa taaaaaaagt acgattaagt gttccttaat cgtactttga
     2641 acgaattcac ccgatgggtg gattgccgat ctgttatttt gtgtccgggt caattcaaat
     2701 cattgttttc ccgctttaat cattaattcg gcgatcttgg ccgcgaactc caccggattc
     2761 tccagtgtaa tcccttccat taacagggat tggccgtaaa ggagctcgtt atactcttta
     2821 aaggtattgg attgaggatc cgcctggtac atgcgttcta gaatgttaaa gacctcgtgg
     2881 tcgggattga tctccagaat ccgggtggcc ttgaacatgg attgattcat ttcggccaga
     2941 atctgttcca tggaaagact gattccggaa tcgccactga ctagacagac cgggctcgat
     3001 ttcaacctgt tggtgagccg gacctcggct actttatcct ttagctgctc cttaatggcc
     3061 ttcagcatcg gctcatgttt ttgggttagt tcctcaagtt ttgccttggc ttctttagca
     3121 gcctcgtcgc cttcgagggc aagatcaccg cgactgaccg atttgaacgg ctgcttctgg
     3181 tattcgtgga gcgagtcgat ggtgaattca tcaacccgat caaagagata gagcacttca
     3241 aagcctttat cgcgcagtga ctcgagttgc ggcaatttct cgactgctgc ccgatccttg
     3301 ccggtaatat agtaaatttc cttctggttt tcgggcatcc gtttcaagta ttcgctcagc
     3361 gtggtgtatc cctcgatagc attggaagag gggaaaagaa gcagatcctg aagccgctca
     3421 tggctttgaa aatcaagata tacgccggct ttgagcgcgc tgccgaattc cttccaaaag
     3481 gtctcgtatt tgggacgttc cttggcaagc atcgactcta aggttttgag cacggtcttt
     3541 tcaagatttt ttccaatcaa cttcaactgc tggctttgtt ggagaatctc ccgggagatg
     3601 ttcagcgaaa agtcggggga atcgactaag ccccgtacga aacggagata atccgggagt
     3661 agctcctcac agttgtccat gacgaaaacg tgtttggagt atagcttggt tccgccgcgg
     3721 tactctttgg agtaaaaatc aaagggtgcc gtggagggga taaagagcag ggcggtgtat
     3781 tccacggccc cttcgacttt ggtatggatc acctcggccg cttcgttcca atcatgaaat
     3841 aggttcttat aaaactgttg atattcctct tgggtgatct cgcttttatt ccgggcccaa
     3901 aggggcgtca tcgaattcag ggtccgggtt tcgatggtga tggtgggagc ggcgccctcg
     3961 atctccttgc cgtccttgtc tttaggccgc tcctccttgg gaaaatcaag cttaatcggg
     4021 taacgaatat aatcggaata ttttttcacc aggttctgaa gggtatagcg gttgagaaag
     4081 ttttcttccg gtttctcggg attacgatag gcttcgttta aggtcaggat gaccgtggtc
     4141 ccgcgtttgg aatgttccgc cggttcaatg gtgtaagttc cgtcgcccgt cgattcccag
     4201 cggaccccat ttgccccggg ggttttggtg agcagggtca ctttgtcgct gaccataaag
     4261 gcagaataga aacctacgcc gaattggccg attaattccg ggttctgact accctcttgg
     4321 ctttgaagtt tttcgatgaa ggccttggtt ccggatttgg cgatggtccc aatgtcttca
     4381 atcacttcat catagttcat tcccaggccg ttgtcggtga tgctcaaagt gtgattggcc
     4441 tcatcgatct cgagccagat ggcgaagtcg gaatctcctt ccagcagatc gaggttggta
     4501 agggattgaa agtgaagctt gtcgatggcg tcggaagcgt tggagatcaa ctcgcgtaaa
     4561 aagatctccc ggttggtata gatggaatgt accatcaaat cgagaagctg cttggtttca
     4621 gcttgaaatt ctttcgtgat tttcgttgaa gtttgatcca ttgccagatt cctcccggag
     4681 tttctagatt ttctcatagg tattttagca tattgatggc tgattgcaac tattttgact
     4741 tgactttcaa tcttttatag aaaaaccggg tcaatgaatg cggttaacga ttggtttaaa
     4801 acttgtcatg agtcacgaat aatgacactg atttttatgt tcacttcttc gtaatggaca
     4861 taaggctatg taccgattat aatgaaattg agaatcgttt tcaaagaccc aatattcatt
     4921 tgcaagattg gactggagag gaaagttgat ttcatccagg agcggtgggg taaattccta
     4981 tattgataat gattatcaat gttgaatgta gaggttaact atcgaaatag aaccggaaaa
     5041 gataaatgaa tagagatcac catccgaagg agccggatca gatgaaacac ccagataaag
     5101 cagaccggga cagccgattc aaacgatttc ttttaaaggc aggtggttat ctgcggcccc
     5161 aaaaagccct ggcagtgatg agatttatcg ggccgggatt tttggtcact atcggtttca
     5221 tcgaccccgg caactgggcc accaatgtag cggccggatc tgagttcggt tatgctctct
     5281 tatgggtgat taccttggcc accgggatgc tgattattct gcagcataat gccgcccacc
     5341 tcgggattgt caccgggtat tgcctgtcgg aggcagccac cttttacctc gccccctggc
     5401 tcagccgggc gattttggtg actgcgatgt ttgcagcagg ggctacggcc ctggcggaga
     5461 tccttggatc aggcatcgct cttaacatgc ttttcggact ccccaccaaa ctgggagccc
     5521 tgctcagtgc cgctctcgtc ttctggttgc ttgagaataa ttcctaccgt aagctcgaga
     5581 aactcatcat cggatttgtg tcgttgattg ggatatcatt tttgattgaa ttgggttacg
     5641 tccacatcca ttggacagag gcgatggtat cctgggttaa acccagccta ccctcaaatt
     5701 cgcttttggc ggtgatgagc ctgttggggg cggtggtgat gccgcacaat atgttcttgc
     5761 attccgaaat cattcaaagt cggcagtgta acctggagaa cgacgcagcc aaaacccggc
     5821 gtcttaaata tgagttcctg gatacctcct ttgccatggt gattggctgg gcgatcaaca
     5881 gcgccatgat tttggtggct gccagtactt tttttagtca agggatccgg gtgaccgatc
     5941 tcagtcaggc ggaacaaatg ctccggccgc ttttgggtaa caccgcggcg gtcatttttg
     6001 ccttggcgct tttatgctcc ggaatctccg ccgcgattac cgccgggatc tccggaggaa
     6061 ccatttgggc cgggatttat ggccggcctt acgacgtgaa agatcgatca tcccggctcg
     6121 gcgcggcact caccattatt ccggctgccc ttgtcatcct gttgatccgc gatacttttc
     6181 aggggttgat ctattcccag ctggcgctgg ggattcagtt gccctttacg atctttctcc
     6241 agatctatat gacctcgtcc cgcagattga tggggaaatt tgtcaactcg aggcttgata
     6301 agatcttact ctggaccatc ggactggtgg tgaccggctt gaatatcgca ctgctggtaa
     6361 tgacggtgaa accctgatgc cgttgaattg ccttcgtttt tccagagtag taggaccccg
     6421 gccaggatca tcaggccacc gcagagttgc atgccgctta aacgttcacc cagaagggaa
     6481 aatgcagtta ttgcagtgaa aaccggttcc agggcggcaa tgagattgac ggtgccggcg
     6541 gggaggtaac caaggctgat attgtataag ccaaaaccaa ttacggtagg cccggcggcc
     6601 aggagaaaca aaacacccca gccagacagg ttttccttca gatagaatat tgattttaag
     6661 ccggttgcac cgggaaaaat ccttaccggt aacaggttac aaagaaataa aaccaatgcg
     6721 gcgaatccga aactataaag cagaacggtc catgggttca gcccgcgtcg cgcagccgaa
     6781 cggcccatca gcgtatatag ggcatagcat agcccggaaa gtattccggt cacgattcca
     6841 gtgagattta cgttccaggc cgcgggattc aacgcccctg caaccatggc gcaacctccc
     6901 agactgaatg cgactgccag gatcttaaac gagttcaagc gttccttcaa aaaacaccag
     6961 cccaaaagca gtgtaaatcc tgtcgaacta taagctaaga cggtggatac ggcagcccca
     7021 ttcaaagcta ctgaaagggt ccacaaggca ttgaaacttg ccagaaccaa cccgaagcca
     7081 cccaggtaaa acaaccggct acggtccaac cggagcagga cgggattgaa aagtcccagg
     7141 acagggagga ggatgaaaaa taccaaacca tcccgccaaa atgccaaaac gagcggaggc
     7201 aaatgatagg attgggtcag gtgacggatg aggatggcgg tagtggataa aaccgccgcg
     7261 ctggccaggg caactagata acccttggta gactttgaat gaattgattt aacttcggac
     7321 aaaaggctcg ctcccttttt ccaggctcgc ttccggat
//
LOCUS       DASVMB010000210         9956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_25918,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000210 DASVMB010000000
VERSION     DASVMB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25918"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VF531_06305"
     CDS             complement(<1..1496)
                     /locus_tag="VF531_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713615.1"
                     /translation="MLKLFRFLKPHRGLVAVVVTLMLLQALSELFLPTLMSDIVDTGI
                     IKGNTGYILRIGGVMLLVAAAGAICAIVAGYFSARVSMGFGKILRNNIFTQVEHFSLH
                     QFDTFSTASLITRTTNDITQIQFVTMMLLRMFITAPIMAAGGVIMAVYLDPELSLVLV
                     AAIPILVLAVWLTAGQSVPLFKIIQLKLDKLNRVLRENLTGIRVIRAFNRTEYEHERF
                     DEANLDLTGTTIRVNQIMAGLMPLMMLVMNFTTIAIIWFGGIRIGAGRMQIGSLMAFL
                     QYAMLIMFSMIIVSMFFVLLPRASASADRINAVLEVIPEFSGPSGSGRDRRGRSAPPV
                     SGDLHRGFLEFNDVSFSYPGAEQPAVSHLSFQAKPGQVTAIIGGTGSGKSTLVSLIPR
                     FYEIDSGAILVDGIDIRQMPPEDLRAKIGFVPQKALLFTGTITENIRYGKPDATDEEV
                     RHAAEIAQATEFISEMKEGFDSLITQGGTNVSGGQKQRLSIARALVRRP"
     gene            1748..3040
                     /locus_tag="VF531_06310"
     CDS             1748..3040
                     /locus_tag="VF531_06310"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7713616.1"
                     /translation="MLTILVVDDEYLVRTGIRETIHWESYNLQIIGEASNGVEGLAAA
                     QSLHPDIIITDIRMPQMDGIEFMAKLREEGLDSKVVVLSGYDEFDYAQSALNYGASAY
                     LLKPIENQQLIETIQKVSRLIHEEKEVKQHYAELKLELPAIQKQFLLDLLDGTITDIR
                     GIEAKIGFLQLPLAFQNQAVVIVSIDDLQILSQRLPHSGIEQLRREIADGISRVLFTG
                     RSFKGLVVMKSVDETVLILQLEDTSGKHLPALKDACKEFTASLENRRDVSVGPAFTVS
                     IGISTPVKDLTELHRAYQEALIGAHTKFLPGTHSVTYIKDEGVHGYHAAVRDAVAFLK
                     NRFSEDITVEMAAKELFISPSYLMHLFKAELGKTFNDCLIEYRIEQAKELLGDKNNRI
                     YEVCQRVGYTDAKYFSQLFKRMTGLSPSEFAKMKQEQE"
     gene            3054..4817
                     /locus_tag="VF531_06315"
     CDS             3054..4817
                     /locus_tag="VF531_06315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7713617.1"
                     /translation="MDNVHGLLRRFKLRNRITLFLVLALIFCSGCLITVSRAVAQSLL
                     KSYLYNYIESTQKEISSSTELIIDEINILAARLTVNDAIYDLFNYQKLSYTNRESQLK
                     RILDRLLVHKELVGAIYIVNRRNEVYEYAPSGGFFQKPDLLDINRTKSSILPVWGQTK
                     RDQANNSYIVFGRKYYNFYTGETLGLLFIYIRESALYNVYRKIVPAGSYSFIIADGKR
                     IISHPDKDQVGSVIFDTALFDVKAPFSYKQVHYNGKPVILAISQFNDNLKSIGINWKI
                     ISIISEKQLFGAIAGINRIVLIIGSVILMVAILLAVYVSFRITESVAGLKHKLDVFGK
                     SPIQAITSGPPRDEIAVLEESYDKMVQRVQELITKNNLEKEKQRELELIALQAQINPH
                     FIYNTLDAIGWIAKLKNQPEIEKLVVALATFFRISLHKGEKFITVEEEIQLVQSFVTI
                     EQMRFPGKFEISYEIPEELKQHYILKIVLQPLVENAIKHGITPKNGMGHIQIHGMIIG
                     DDLIFEISDDGVGFEMNTANFDISYQKTKYSGYGLRNVNERIVLEYGPDYGIGFESEK
                     HKGTTVRVKLRDTGKRDVEPA"
     gene            complement(4814..5083)
                     /locus_tag="VF531_06320"
     CDS             complement(4814..5083)
                     /locus_tag="VF531_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713618.1"
                     /translation="MRARTQASDSYLAANRGVLYLSYQIPHSASSIVKVVSLNQVRFG
                     LWGFLCLLILLNAGLIILIVIFSRTLSQSISRPLAALFQKMQQKI"
     gene            complement(5120..5815)
                     /locus_tag="VF531_06325"
     CDS             complement(5120..5815)
                     /locus_tag="VF531_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713619.1"
                     /translation="MFTRLERRIRMMLILIVSFSLISSSLLLYLALQRNIFANYVQLA
                     TQYANQQNRNADLYLSLIEETAKLLSGDEDIIRALQNPRFDSALISRAVAKLSGIQTG
                     SVTLTGITMYGLNEVCYASESIGLAPNNPPTLKQLMADPLFREFAGSEQRILWWVRYQ
                     KAIRFPVYNSNIHGGLITLVLKIYDPNNKPVGFLLVDTKVNSFFLFFKDKEDSDGAFL
                     IGGAHEVVAASYN"
     gene            5968..7380
                     /locus_tag="VF531_06330"
     CDS             5968..7380
                     /locus_tag="VF531_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713620.1"
                     /translation="MSRKWSLISILLILTAGLSIFAVQHLKPQQKEVVISVGDWPGEN
                     GPRHQIYERYARMIKEQRRITVVPDEWSYSINSFLPKAAAGKLPTLYNTWFTETGKII
                     EAGYAAEITPQLAKRGWDRALSPETSRVVKRNGKIYGVPRDAYLMGLMCNLKLFRKAG
                     LLDSAGLPQIPRTYAELARTAKLIKDRTGAAGFLIETSSNGGGWHFMNIAWSFGAEFE
                     RKVNGRWQAVFNSPQAVAALEYIRDLRWKYDLLPNLALIDWAKGQELIGTGQCAMKLE
                     AGFEHNGFDNVIDNYHMNKDLISMGSVPAGSAGRMALLGGNIYMFAPNASTEQINAAL
                     EWIKLLGQSPDVSGAGLKDLELAYRNDYQAGYVVGNPGIPVWVNPGFTRANQRIRSRY
                     SNVNPDNFRDYRDFTQVKIHSEEPVNCQELYSLLDRVIQTVLTDKSANPQALLNQAVR
                     EFQKAYLDKANRSILNQPQS"
     gene            7396..9702
                     /locus_tag="VF531_06335"
     CDS             7396..9702
                     /locus_tag="VF531_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX7713621.1"
                     /translation="MKAGHRYLDVDPWVIRETGFHPEQGRVSESVFSLGNEYMGVRGY
                     FDEGYDGDRLVGSYCNGFYEEAPVYQASSYRGLISRARFMVNTVDWLFTRICVDGETL
                     DLAHSRVSDFQRVLDFKTGCLTREFVWETSSGKSLRVIFRRLVSMAEPHLGCQQLTVT
                     ALDFSGTVEIQSGLDFSILHEEKRQNFWSCPRRVTENGLVAILGKTIHLGHQLFSAFR
                     LETDAPIAPRLVEREKYIGLKYILDLTEGMPVTVEKIVTNYAERNSADRPADAWNRGM
                     ELAREYRRLNFDGALREHQAYWERVWNDLDITIEGDPENQQGIRFCIFQLHQTFHGTD
                     PGLNIGAKGLTGEAYNGHTFWDTETYCLPFYIFNNPKAARNLLEFRYRTLPQAKERAI
                     ELDSKGAFYPIATIDGTESCGLWQHASLQLQVGSAVAYGIWHYVKLCRDKEFLYQKGI
                     EILIEISRCYAGRGQWGQRTGEFGFFGVMGPDEFHMMVNNNCYLNFMARKTFAFTLGT
                     LGEMESACPGQLEAIRRKTHLESSEISDWRRITAKMRLPQDPTTGVYEEHDGFFDLPH
                     LEANSIPETEFPLYEHWSYDRIYRYDLIKQPDVLMFIFLYNQDFSEDIKRANYQYYEP
                     RCIHESSLSPSIHSILGAELGYHERAFDLCRFATRLDLDNYNRNTSQGLHITSLAAAW
                     MNIVYGFGGMRSDGERLLFNPTIPASWQAYSFRIIYQGANLCLNVNRESVELRSLNGV
                     KASVRIYGADYQADDKGVKIQIPDSYRV"
BASE COUNT         2556 a         2335 c         2619 g         2446 t
ORIGIN      
        1 ggcctgcgaa ccagagcccg ggcgatggaa aggcgttgtt tctgtccgcc ggagacgttg
       61 gtgcctcctt gggtaatcag ggagtcaaat ccttctttca tctcggagat aaactcggtg
      121 gcctgggcga tctccgcggc atgccgcact tcctcatcgg tggcatccgg tttgccataa
      181 cgaatatttt cggtgatggt accggtgaaa agcagagctt tttgtggaac aaaaccgatc
      241 ttggcccgca gatcctcggg cggcatctgc cgaatatcta ttccatccac gagtatggcc
      301 ccgctatcga tttcataaaa acgtggaatc aggctgacca gcgttgactt gccggaaccg
      361 gtcccaccga tgatcgctgt cacctggccg ggttttgcct ggaaagagag atgactcacc
      421 gccggttgtt cggctccggg ataactgaag ctcacatcgt taaattccag aaagccccga
      481 tgtaaatcgc cggaaacggg gggggcggaa cggcctctcc ggtccctgcc cgacccgctc
      541 ggacccgaga actccggaat cacctccaaa accgcattga tccggtctgc cgatgccgat
      601 gccctgggta ataaaacgaa gaacattgaa acgataatca tggagaacat gatcaacatt
      661 gcgtattgta aaaaggccat tagtgatccg atttgcatcc gtcccgctcc gatccggatt
      721 cctccgaacc agataatggc gatggttgta aaattcatga ccagcatcat caggggcatc
      781 aaccccgcca tgatttgatt aacccggatg gtggttccgg tcagatcgag attggcctcg
      841 tcgaaacgtt cgtgttcgta ttcggtgcgg ttaaaggcgc ggatcacccg gattccggtt
      901 aagttctcac gtaatacccg gtttagctta tcaagcttga gttgaatgat tttgaacagc
      961 gggacgctct ggccggcggt cagccaaacc gccaggacca ggatgggaat ggcagccacc
     1021 aataccagcg agagctcagg atcaaggtac actgccatga tgaccccgcc cgcagccata
     1081 atcggtgcgg taatgaacat ccgtaataac atcatggtca cgaattgtat ctgagtgatg
     1141 tcattggtgg tccgggtgat cagcgaggcg gtactgaatg tgtcgaactg gtgaagggaa
     1201 aaatgctcca cttgagtaaa gatattattc cgcaagatct ttccaaaccc catggaaacc
     1261 cgggctgaaa agtaccccgc cactatagca catatagctc cggcagccgc caccagcaac
     1321 ataaccccgc cgattcgcag gatatatccg gtgtttcctt tgatgattcc ggtgtcgaca
     1381 atatccgaca tcagggtcgg caagaacaat tcggagagag cctgaagcaa catcaatgtc
     1441 actaccaccg ccaccaagcc ccggtggggt tttaaaaatc ggaacaactt cagcatgcct
     1501 tcatctccgg taattcgatt ttgcaaacgg atattcccca tgaaatcgtc ccggttagca
     1561 tccccatcaa cccatggaaa taaaactatt agcttgggtt catccagagg ggagggattc
     1621 cttgctttgg gtatcgtctt tagctgcccc gccggatccg ggcttgaaaa tgccgggcag
     1681 gatcttgggg tggatttggc gaaatgttaa agatagaatg tattggttcc ggaaaggagc
     1741 accccgattg ttgacgatct tggttgtgga cgatgagtat ctggtccgga ccggcatccg
     1801 ggaaacgatt cattgggaga gttataacct tcaaatcatc ggggaagcga gcaacggggt
     1861 ggaaggactc gctgcagccc aatccctgca tcccgatatt atcatcacgg atattcgcat
     1921 gccgcagatg gatggaattg agtttatggc aaaactccgg gaggagggtc tcgactccaa
     1981 agtcgtcgtt ttaagcgggt atgatgagtt cgattatgct caatccgcct tgaactacgg
     2041 cgcttcggct tacttactca agcctattga gaatcaacag ctgatcgaga cgattcagaa
     2101 agtcagccgc ttgattcatg aggaaaagga agttaaacag cattatgccg agttgaaact
     2161 ggaactcccc gctattcaaa aacagttcct tctggacctg ctggacggaa ccattaccga
     2221 catccggggg atcgaggcca aaatcggatt tttacagttg cccctggcct ttcagaacca
     2281 ggcggtcgtg attgtaagta tcgatgattt gcagatcctc tcgcaaaggt tgccacattc
     2341 cggtatcgaa cagctacgcc gggagatcgc tgacggaata tcccgggtgc tcttcaccgg
     2401 gagaagcttt aagggcctgg tcgtcatgaa gagcgtggat gagaccgttc tgattttgca
     2461 gcttgaagat accagcggga agcaccttcc tgcgcttaag gatgcctgca aagaatttac
     2521 ggcaagtctt gaaaaccgaa gggatgtttc tgtcggacct gcattcaccg tttctatcgg
     2581 tatcagcacc cctgtaaagg acctaaccga acttcataga gcctatcagg aagcgttgat
     2641 cggagctcac accaaatttc tgcccgggac tcacagcgtc acctatatca aagatgaggg
     2701 ggtccatggt taccatgctg ccgtaaggga cgcggtcgcc tttttgaaaa accgtttctc
     2761 cgaagatatc accgtggaga tggcggccaa agagctgttc atcagcccct cttacttaat
     2821 gcacttattc aaagcggagt tgggcaagac atttaatgac tgcctgatcg aatacaggat
     2881 tgaacaagcg aaggagttat tgggagacaa gaacaatagg atttacgagg tttgtcagcg
     2941 ggtgggctat accgatgcca agtatttcag ccaattattc aagcgaatga ccgggttgag
     3001 tccgagtgag ttcgccaaga tgaagcaaga gcaagagtaa aatggggaaa accatggata
     3061 acgttcacgg tcttttaaga cggttcaaac ttcgcaaccg gatcacactc tttttggtgt
     3121 tggccctgat attctgttcc gggtgcctga tcaccgtctc gcgcgcggtt gcccagtctt
     3181 tgctcaaaag ttacctttac aattatatcg aatccaccca aaaggagatc tcgtcaagca
     3241 ccgagttaat catcgacgag atcaacattt tagcggctcg gcttaccgtg aatgacgcca
     3301 tctatgatct tttcaactat caaaagctct cctacacaaa ccgcgagtcc cagttgaaac
     3361 ggatccttga tcgtttactg gttcataagg agctggtcgg cgcaatttat atcgtcaacc
     3421 gccggaacga ggtttacgaa tacgctcctt ccggcggatt ctttcaaaag ccagatctgc
     3481 tcgatatcaa ccgtaccaaa tcctcaatct tgcccgtctg gggccaaacc aaacgggatc
     3541 aggccaacaa ctcctacatc gttttcggac gcaaatatta taatttttat accggggaaa
     3601 ccctgggctt actcttcata tatatccgtg aatcggcact ctacaatgtt tatcggaaga
     3661 ttgtgcctgc gggcagttat tcatttatca tcgccgatgg gaagcgaatc atctcccatc
     3721 cggataagga tcaagtcggc tcggtcatct ttgacacggc gctttttgac gtgaaggcgc
     3781 ctttcagtta caaacaagtt cattataacg gcaaaccggt gatcctggcc atctcccagt
     3841 ttaatgacaa tctgaaaagc ataggtatca attggaagat catcagtatc atctcggaga
     3901 aacaactctt tggcgcgatt gccggaatca accggattgt gttaatcatc ggttcggtga
     3961 ttctcatggt agccatttta ctggcggttt atgtttcgtt ccggatcacc gaatccgtgg
     4021 ccgggctcaa gcataaactg gacgttttcg ggaaatcccc cattcaagcc ataacttccg
     4081 gcccgccccg ggatgagatc gcggttctcg aagagagcta cgataaaatg gtccaaaggg
     4141 ttcaagagct cattaccaag aacaacctgg agaaagagaa gcagcgggag ttggaactca
     4201 ttgccctgca ggcccagatt aacccgcatt tcatctataa tacactggat gccatcggct
     4261 ggattgcgaa actcaagaat caaccggaga tcgagaaatt ggtggtcgcc ctggccactt
     4321 tcttccggat cagtcttcat aaaggcgaga agttcatcac tgtcgaagaa gagatccagc
     4381 tggttcagag ttttgttacc attgaacaga tgcgttttcc ggggaagttt gagattagtt
     4441 acgagatccc ggaggagttg aagcagcatt atattctgaa gattgtcctg caacccctgg
     4501 tggaaaatgc gatcaagcat gggatcaccc ctaaaaacgg gatgggtcat atccaaatcc
     4561 acgggatgat catcggtgac gatctgatct ttgagataag cgacgacggg gtgggttttg
     4621 agatgaatac cgcaaatttt gatatttcgt atcaaaagac caaatatagc ggttacgggt
     4681 tacgtaatgt gaacgaacgg atcgtgcttg aatatggacc ggattacggg atcgggtttg
     4741 aaagtgagaa gcacaaagga accacggtcc gggtcaaact tcgtgatacc ggcaagcgag
     4801 atgttgaacc ggcttaaatt ttctgttgca tcttttgaaa gagcgcggcc agtggccggg
     4861 agatgctctg ggacagtgtt cggctgaaga ttacgattaa aatgatcaga cctgcgttta
     4921 aaagaataag taagcaaaga aaaccccaga ggccgaaacg aacctggttc agtgaaacaa
     4981 cctttacaat acttgatgcg gaatgcggta tctggtatga aagatataag actccacggt
     5041 tggcggcaag ataagaatcg gatgcctgtg tgcgagcccg catcttttgg agatcctctg
     5101 ttgcctttgg cggcaaccct taattataag acgcagccac tacctcgtga gccccgccaa
     5161 tcagaaatgc tccgtccgaa tcctccttat ccttaaaaaa gagaaagaat gaattgactt
     5221 tggtatccac cagcagaaat ccgaccggtt tgttgttggg atcgtaaatc ttgagaacca
     5281 gtgtgattaa cccgccatga atattcgaat tatatacggg aaatctgatc gccttttggt
     5341 agcgaaccca ccacaagatt cgttgttcgg aaccggcgaa ctcccggaac aggggatcgg
     5401 ccattaattg cttaagtgtc gggggattgt tgggagcgag tccgatggac tccgaggcat
     5461 aacaaacctc atttaggccg tacatcgtga taccggtaag tgtgacacta cccgtttgaa
     5521 tcccgctaag cttggccacg gcccggctga tcaaggcgct gtcgaacctt ggattttgca
     5581 gggcacgaat gatgtcttcg tcgccggaga gaagtttagc ggtttcctcg attaggctta
     5641 aatagagatc ggcgttacgg ttttgttgat ttgcatactg ggtggccagc tgaacgtaat
     5701 tggcaaagat gttgcgctgg agcgccagat agagtaagag gctggagctg attagactaa
     5761 atgagacgat caggatcaac atcatccgga ttctccgttc aagccgggta aacaagagaa
     5821 caccaccccc tacttcactc tattgtaaca ttttatttgc gataatgaaa cttagcagtc
     5881 tgacaaacca tttagcaggt ttaccgagtt aaaatcgtcg ggtatttcgt ttacaataaa
     5941 tatgtggtca agaattggag gggttcgatg tcccgaaaat ggtcattgat tagcatctta
     6001 cttatcttga ctgccggttt atcgattttt gccgtgcaac acttgaaacc gcaacaaaaa
     6061 gaagtggtaa tctcggtcgg ggactggcct ggtgaaaatg gaccgcgtca ccagatctat
     6121 gaaagatatg cccgaatgat aaaggagcag cgccggatta cggttgtacc ggacgaatgg
     6181 tcgtattcga tcaactcttt tttaccgaaa gcggccgcgg gaaaattacc tacgctttat
     6241 aacacctggt ttaccgagac cgggaagatc atcgaggcgg gttacgccgc cgagatcact
     6301 ccgcaactgg cgaaacgggg atgggatcgg gcgctcagcc ctgagacatc aagggtagtt
     6361 aagcgaaacg gcaagatata cggtgtgccg cgagatgcct acttgatggg cttgatgtgt
     6421 aatctcaagt tatttaggaa agccggtttg ctggactccg ccggtttgcc gcaaatcccg
     6481 cgaacctatg ccgaactggc acggaccgcg aaattaatca aagaccggac cggagccgcg
     6541 ggtttcttaa ttgagaccag cagtaacggg ggcggctggc atttcatgaa catcgcctgg
     6601 agctttgggg cggagtttga aaggaaagtc aacggccgct ggcaggcggt atttaactca
     6661 ccgcaggctg tggcggcttt agagtatata cgcgatctgc gctggaaata cgatctcctt
     6721 ccaaatctgg ctttaatcga ttgggccaag ggtcaggagt tgatcgggac cggtcaatgc
     6781 gccatgaaac tcgaggccgg ctttgaacat aacgggtttg ataatgtcat cgataattac
     6841 catatgaata aagacttgat ttccatggga agcgtccctg caggatccgc gggacgaatg
     6901 gcattgctcg gcggaaatat ttatatgttc gcccccaatg cctctaccga gcagattaat
     6961 gccgccctgg agtggatcaa actcctggga cagagccccg atgtttcggg ggcggggttg
     7021 aaagatctgg aactggctta ccgtaacgat tatcaagccg gatatgtggt gggcaatccc
     7081 gggatcccgg tttgggtcaa tcccggtttt accagagcca atcaaaggat acgttcccgt
     7141 tattccaatg tcaatccgga taatttccgt gattaccggg atttcacaca ggtgaagatc
     7201 cattccgaag aaccggtaaa ctgtcaagag ttatatagct tactcgatcg ggtaatccag
     7261 acggttttaa cggataaaag cgccaatccc caggctctgc tgaaccaggc ggtcagggaa
     7321 ttccaaaagg cttatctgga taaagccaat cggagtattc tgaaccaacc gcaatcatga
     7381 taggagtgac ttttgttgaa agcaggccat cgttatttag atgttgatcc ttgggtgatc
     7441 agggagaccg ggtttcaccc cgagcagggc cgggtatcgg agtccgtttt ttcgctgggc
     7501 aatgagtata tgggtgttcg gggttatttt gacgaggggt atgacggaga ccggttggtt
     7561 ggaagttatt gtaacggttt ttacgaggaa gcaccggttt atcaggcttc ttcatatcgg
     7621 ggtttgatct cgcgggcgcg gtttatggtc aataccgtgg attggctttt tacccggatt
     7681 tgcgttgatg gtgagacact ggatctggct catagccggg ttagcgattt tcaacgggtg
     7741 ctggacttta aaaccggttg tttgacccgg gaattcgttt gggaaaccag cagcggcaaa
     7801 tcgctcaggg tgatcttccg tcgcctggta agcatggcag agccgcatct gggttgccag
     7861 cagttgacag taacggcgct ggatttctcc ggaacggtgg aaatccaatc ggggttggac
     7921 ttttcaatcc ttcacgaaga aaaacgccaa aacttctgga gttgcccgcg aagggttaca
     7981 gagaatggtt tggttgccat attgggaaag acgatccatc tcgggcatca gcttttttcc
     8041 gccttccggc tcgaaaccga tgcccccatc gccccccgtc tggtggagcg ggagaagtat
     8101 atcgggctaa agtatatcct ggatcttacg gaaggaatgc cggtaacggt cgaaaagata
     8161 gtgaccaatt acgcggaacg caattccgcc gataggccgg ccgacgcttg gaaccgcgga
     8221 atggaactgg cccgggaata ccgcaggcta aatttcgatg gggcgttacg ggaacaccaa
     8281 gcctactggg aacgggtatg gaatgacctg gacattacca ttgaaggtga tccggaaaat
     8341 cagcaaggta tccggttttg catctttcaa ttacatcaaa ctttccatgg gactgatccc
     8401 ggcctcaata tcggggctaa aggattgacc ggggaagctt ataatggtca taccttttgg
     8461 gataccgaaa cctattgcct gccgttttat atctttaaca atcccaaagc cgcccggaac
     8521 ctgctcgagt tccgttaccg gactctgccg caagcaaagg aacgggcgat agagctcgat
     8581 tcaaaagggg cgttctatcc gatagccacc attgacggca ctgagagctg cggactttgg
     8641 caacatgcca gtttgcaatt acaagtcggt tcggcggtgg cgtacgggat ctggcattat
     8701 gtgaagctct gccgggataa ggaattctta tatcaaaagg gtattgagat tttgattgag
     8761 attagtcgtt gttacgcagg gcggggccag tggggtcagc gtaccgggga gttcggcttt
     8821 ttcggagtga tgggtcccga tgaattccat atgatggtca acaacaactg ctacttaaat
     8881 ttcatggccc ggaagacctt tgcctttacc ttgggaaccc tgggggagat ggagtctgcc
     8941 tgccccgggc agttggaagc gatccggcgc aagacccatc tcgaatcgtc ggaaatctcg
     9001 gattggcggc ggataaccgc caagatgcgg ttgccgcagg atccgaccac cggggtctac
     9061 gaggaacacg acggtttctt cgatctgccg catctcgagg ccaattccat cccggagacg
     9121 gaatttcccc tctatgagca ctggtcctat gaccgtattt atcgttacga tctgatcaag
     9181 cagccggatg tattgatgtt tatcttcctg tataatcagg atttttctga agacatcaaa
     9241 cgcgccaatt atcagtatta tgagccccgg tgtattcatg aatcgtcgct gtcgccttcg
     9301 attcattcga tcctcggagc ggaattgggt tatcacgaac gggcctttga tctttgccgc
     9361 tttgcaaccc gtttggacct tgacaattat aaccgtaata cttcgcaggg gttgcatatc
     9421 acttccctgg ctgccgcctg gatgaatatc gtatacgggt tcggcgggat gcgctccgac
     9481 ggtgagaggt tgcttttcaa tccgacgatt cctgcatctt ggcaagctta tagtttcaga
     9541 atcatctatc agggagccaa tctttgtctc aacgtcaacc gggaatcggt tgagcttcgt
     9601 tcgctgaacg gagtcaaagc ttcagttcgg atatacggag cggattatca agcggacgat
     9661 aaaggggtaa aaatccaaat tcccgattcg tatcgcgttt aagcagtaac acaataaaaa
     9721 gggctgttct aaaagaggta gagttctgct ctatttatgg tagaaaatta ctttatttat
     9781 ataatttgta aataacatcg gtttcagcgt tgtcgcgacc gctgcaaccc ttcttacgtc
     9841 gatttcttta cgaaatagag atcaccattt gatagaaagg ggggatgccg ggaaatattg
     9901 caaatcgatc tgggacaaag aacaaatata aggaggatga agcttttgag taaagt
//
LOCUS       DASVMB010000211         5848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_248052,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000211 DASVMB010000000
VERSION     DASVMB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248052"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1246)
                     /locus_tag="VF531_06340"
     CDS             complement(125..1246)
                     /locus_tag="VF531_06340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7713622.1"
                     /translation="MKPIFILLTATLLLGLTTFPGMAQQPPEPTASAAILMDYTTGKI
                     LYQKNAYERRFPASTTKILTAIVAIEKGNLKKIITADATAAKTDGSSIWLKAGERQTL
                     KDLLYGILLNSGNDACVAVADSLAGSEKRFTSWMNTKAYEIGAKDSHFANSNGLPNPV
                     HFTTAYDLSLITRYAMKNPVFAEIVRTKRKTIPWPGRDYDRVMINHNKLLWRYEYADG
                     VKTGYTMEAGKCLVSSATKDNHRLIAVVLNSRDMYGDSQRLFDYGFSHYQLLSLASPQ
                     ETLSQVNVTEGIASQVPVSANRPLNLLIPKGDESKLRVNLELRSSLRAPVERMQQVGE
                     LQVKMGERLIEKVPVVTLARVPQKSWWRRIWDWLRGFIG"
     gene            complement(1484..1915)
                     /gene="ytfJ"
                     /locus_tag="VF531_06345"
     CDS             complement(1484..1915)
                     /gene="ytfJ"
                     /locus_tag="VF531_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HEX7713623.1"
                     /translation="MNEHPIEGLMKTAMQNIKEMVDVNTILGDPVETVDGSVIIPISK
                     VTFGFAAGGSEFEAIQGKPNNKERERSSENADAAANLPFGGGSGAGITIHPVAFLVVG
                     KEQTKLLPVESNVLVDRILDSAPQLLDRLQNIISGNRSEVH"
     gene            complement(1912..2415)
                     /locus_tag="VF531_06350"
     CDS             complement(1912..2415)
                     /locus_tag="VF531_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HEX7713624.1"
                     /translation="MKSRQHLSGRWFFIRQSQTKTVKAPYSISSRGEREFLRRYAHYG
                     LAVTLLTYFLPANYFHWLLVAERLEHKGWFSKFYWATRLGTGDPALTAIGYGIVWGLK
                     TNLLSYLDSRYHFKERPELKVVPDYQQPRWDMLFDCIFRVKIGYIIIASLAARWRLRM
                     LKGGVGN"
     gene            complement(2693..5401)
                     /locus_tag="VF531_06355"
     CDS             complement(2693..5401)
                     /locus_tag="VF531_06355"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HEX7713625.1"
                     /translation="MGNCFGIEASEVARIFGADLKRGLTTTEAQKRLVRTGPNQLQTI
                     KKDSAWQILGEQFTDFMVLVLIGAAIVSFLLGERADALTILAIIILNAILGFIQEYRA
                     ERSLAALKELTAPLAHVLRNSKVVRIPAKELVPGDILVLESGDRIPADARLFETIDLE
                     VNEATLTGESLSVVKHTDLVSASHPALGDQFNMVFAGTVVTNGRGRAVATATGMQTEI
                     GKIADLIDTSQSDATPLQKKLEQLGRWLVLICLGICAAVASIGILRGEPPRQMFLAAV
                     SLAVAAIPEGLPAIVTIALAIGVQKMIKRQAIIRKLPAVETLGCATVICSDKTGTLTQ
                     NKLAVTRLWAGGSEQNLSREKKASPPVWSKELHKLLEIAVLCNNAQLETEHGHEVVYG
                     DPTEAALLTAGVEGGIDPRLLAKNRPRRGELPFSSDRKRMSVWVDSGTDGLEFMIKGA
                     ADIILERCDRILTTRGIEPINHIAISQIKKRVEEWGSEALRVLAFAYRPADSAELNRK
                     NGDPETGLIWAGFIGMTDPPRPESRAAIARCKRAGIKTKMITGDYSRTAAAVAKEIGL
                     LRPTGKIMSGTDLDNLSDVELTRRIESIDVFARVSPHHKLRIVQALKRNGEIVAMTGD
                     GVNDAPAVKDANIGVAMGQNGTDVTKEASAMVIADDNFATIVAAVEEGRAIYENIRKF
                     IRYLLSCNTGEILTIFGGIMLGLPFPLLPIQILWVNLVTDGLPAIALGMEPAEPGIMQ
                     RPPRDPHEGIFSRGLGWKIVFQGFVIGAVTLLIYLFELKSGAGIGKARTAAFGALVFA
                     QLFFVFQCRSESRSMLQMNPLRSRYLLGAVLISGTMQVLVMVISSLQRIFYTSGLNYW
                     EWILIVVLTGFSAVISDFIFRFRSRIRKHFTFFRFEVHRLTNQAGK"
     gene            5562..>5848
                     /locus_tag="VF531_06360"
     CDS             5562..>5848
                     /locus_tag="VF531_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX7713626.1"
                     /translation="MPIDAPAIGCLSRELKHYLPVKIDKIWQPYPDEFIFSCFGSGAS
                     FKLLLSVHPQFGRFHLFSGTRENPINPSAFCMLLRKHLGGAKLVTVDAVPF"
BASE COUNT         1497 a         1542 c         1336 g         1473 t
ORIGIN      
        1 atcctaagtc tagcgcgtct gccattccgc cactctcgca ttgtataaag attatagcac
       61 ccagccggta ttcagtcaat agatactaaa taacgagacc agctacttcc ggatgaacca
      121 ctcgttaacc gatgaaacct cgaagccaat cccaaattcg tcgccaccaa cttttttggg
      181 gaactcgcgc cagagttacc acaggaactt tttcaattaa ccgttccccc attttcacct
      241 gcaattcacc aacctgctgc atcctttcga ccggagcccg gagtgagctt cgcaattcca
      301 gattgacccg gagtttcgac tcatctcctt ttgggatcag caggtttaat ggacgatttg
      361 ctgaaaccgg tacctgggaa gcgatgccct cggtgacatt aacctggctc agcgtctcct
      421 ggggtgaagc cagactgagc aattggtaat gggaaaaacc ataatcaaat aatctttgag
      481 aatcaccata catatcgcgg ctgtttaaaa ccacggcaat caaacgatga ttatcctttg
      541 tggccgagga aaccaggcat ttaccagcct ccatggtata accggtttta actccgtctg
      601 cgtattcata acgccataat agtttgttat gattaatcat tacccgatcg taatcccggc
      661 ctggccaggg aattgtcttt cgtttcgtac ggacaatctc cgcgaatacc gggtttttca
      721 tggcataccg ggtaatcaag gataaatcgt aagcggtggt gaaatgaacc ggattgggaa
      781 gaccgttgct attggcgaaa tggctatctt tggcgccaat ctcatatgcc ttggtgttca
      841 tccaggatgt aaaccgtttc tccgaacctg ccaggctgtc ggctacggcg acacaagcat
      901 catttcccga gttgagcagt atcccatata atagatcctt gagtgtctgg cgttcaccgg
      961 ctttaagcca gatggatgaa ccatcggttt tggcagcggt ggcgtcagcc gtgattattt
     1021 ttttaaggtt tcccttttca atcgccacaa tcgccgtgag gatcttggtg gtgctggccg
     1081 gaaaacgcct ttcgtaggca tttttctgat acagaatctt gccggtggta taatccatta
     1141 aaatcgcagc cgatgcggtt ggttcgggcg gttgctgagc catccccgga aaggttgtca
     1201 agcccaataa taatgtagcc gttaataaaa taaatatcgg tttcacgcga ctcgctccat
     1261 cccacggatt tgttctacca gaccggtccg gttaaagata attactgtta aaaaggcttt
     1321 tccggttaga taattcgaaa ccggtcctta aatcccttcc gattttggat aaaatggatt
     1381 ggaaattaaa aaccgcgagt agaccgcggt ttttaaactt gattcggctg ttctttttgt
     1441 atcctgtcaa aaaaaacaca ataaaattgt gtcaaatcga agttcaatga acttcgctcc
     1501 gatttcctga aatgatgttt tggagtctat ctaacaattg gggggccgaa tctaaaatgc
     1561 gatcaaccag tacattactc tcaaccggca gaagtttggt ttgttccttg cctactacca
     1621 aaaaagccac cggatgaatg gtgatcccgg cgccactgcc tccgccaaat ggaagatttg
     1681 cggcagcatc ggcgttttcg gaacttcgtt cacgctcttt attattgggc ttgccttgaa
     1741 tcgcttcaaa ttcactaccg cctgcggcaa agccgaacgt tactttggaa attggaatga
     1801 ttacgcttcc gtcaactgtt tcaactggat cgcccaggat agtgttgaca tcaaccatct
     1861 ctttgatgtt ctgcatcgcg gttttcatta aaccttcaat cggatgctcg ttcaattccc
     1921 gacaccccct ttcaacattc gtaaacgcca tcgggccgcc aatgaagcaa taataatata
     1981 gccgatcttt actctgaata tacagtcgaa gagcatgtcc catcttggtt gctgataatc
     2041 ggggaccact ttgagttcag ggcgttcctt gaaatggtag cggctgtcga gataactcag
     2101 tagattcgtt ttcaaccccc acacgatccc gtaaccaatg gctgttaacg caggatctcc
     2161 ggttcccaaa cgagtcgccc aataaaattt ggaaaaccat cccttatgct ccaaccgttc
     2221 cgctaccagc aaccagtgaa aataattcgc cggcaaaaag taggtcaaaa gtgtaacagc
     2281 caacccataa tgagcgtacc ggcgcagaaa ttcccgctcg ccgcgggaac ttatactata
     2341 cggcgctttc acggtttttg tttgggactg gcggataaag aaccatctgc cggaaagatg
     2401 ctgccgactt ttcatggact ccggagtcgg tttgaacaga ctcaccgctt tagtgcgttt
     2461 taataggcct ccgagaaaag acatctctag ggtaagcgaa tcatcccacc cgagtttgga
     2521 ataacgaaag tggaactcaa gcggtagaaa aaaaagagcg caaaatgtcc ccaagaacaa
     2581 gaccaataca atcacagacc accccgatgc tcgcgcgtag cgcacccgtc ataaagaccc
     2641 ttggcaaact taagtttttt aaaaacagga tgggtgaaac actaatttat ggttattttc
     2701 ccgcttgatt ggtgagccta tgcacttcga agcgaaagaa agtaaaatgt ttccggatcc
     2761 ggcttcgaaa acgaaagata aaatcggaga ttaccgcgga aaaaccggtc agcaccacga
     2821 tcaaaatcca ttcccagtag tttaggccgg atgtgtaaaa gatccgctgc aatgaggaga
     2881 taaccatcac caaaacctgc atcgtcccgg agatcaacac cgcacccaat agataacggc
     2941 ttcttagcgg attcatctgc aacatggaac ggctctcgga acgacattga aaaacgaaaa
     3001 aaagctgagc aaagaccaat gccccgaaag cggcggtcct ggcttttccg attccggcac
     3061 ctgatttcaa ttcaaaaaga tagataagta gagtcacagc cccgatgaca aaaccttgaa
     3121 agacaatctt ccatcccaaa ccgcgactga aaatcccctc atggggatcc cgcggcggac
     3181 gttgcataat ccccggttcg gcgggttcca ttcctagcgc aatggcaggt aaaccgtcgg
     3241 ttaccaggtt aacccataaa atctgaatcg gcaaaagtgg aaatggcaaa cccaacataa
     3301 ttccgccgaa aattgtcaga atctcgccgg tattacagct taatagatag cgaatgaact
     3361 tgcgaatatt ttcataaata gcccggcctt cctctacggc cgccacgatg gtggcaaaat
     3421 tatcatccgc gataaccatg gccgaagcct ctttggtaac atcagtgcca ttttgaccca
     3481 tggccacacc gatgttggca tccttgacgg ccggggcgtc attaacaccg tctccggtca
     3541 ttgccacaat ctcaccgttt cgctttaggg cttgcacgat ccgtaactta tgatggggag
     3601 ataccctggc aaaaacatcg attgattcga tccgccgggt caactcaacg tcggataaat
     3661 tatccagatc cgttccactc atgatctttc ctgtggggcg aagcaaaccg atttctttag
     3721 ctactgcagc cgcagttctt gagtagtccc cggtaatcat tttggtcttg atcccggccc
     3781 tcttacaacg ggcaatggct gcccgggatt ccggccgcgg cggatcggtc ataccgatga
     3841 atcctgccca gatcaggccg gtttccggat cgccattctt ccgattcagt tccgcggaat
     3901 ctgccgggcg gtaagcaaaa gccagaacgc gcaaagcctc cgaaccccac tcctcaaccc
     3961 gctttttgat ttgggagatt gcgatgtggt taatcggttc tatacctctg gtggttaaga
     4021 ttcggtcgca gcgttccaag ataatgtctg cggctccctt gatcataaat tccagcccat
     4081 ctgttcccga atccacccaa acgctcatcc tcttccggtc ggaggagaaa ggcagttcgc
     4141 cacggcgagg gcgatttttg gccaaaagcc ggggatcaat acctccttct actccggcag
     4201 taaggagtgc tgcttcagtc ggatcgccgt aaactacttc atggccatgc tccgtttcga
     4261 gctgggcgtt attacataga acggcaatct ccaacaattt gtgcaattct ttagaccaga
     4321 cgggggggga cgctttcttt tcccgggata ggttctgttc ggacccaccc gcccataggc
     4381 gggtaactgc caatttgttt tgagtcaggg ttccggtttt gtcggagcaa atcaccgtcg
     4441 cgcaacccaa tgtctctaca gcgggcaact tgcgaatgat cgcctggcgc ttgatcatct
     4501 tttgaacccc aattgccaaa gcgatggtga caatggccgg taaaccttca ggaatggccg
     4561 ccacggccag actgacggct gccagaaaca tctgccgggg cggctcgccc cggaggatcc
     4621 ctatactggc gacagcagca caaattccca gacagatgag aaccaaccac cggcccagtt
     4681 gttccagttt cttctggagc ggagtggcat cggattggct ggtatcgata agatcggcga
     4741 tcttgccgat ctctgtttgc atcccggtgg ccgttgccac cgcccgcccc cggccattgg
     4801 tcaccactgt ccccgcaaaa accatgttga attgatcacc gagtgcggga tggcttgcgc
     4861 ttacaagatc ggtatgctta actactgaaa gtgattcacc ggtcaaggta gcctcattta
     4921 cttcaaggtc gatggtttca aaaagccgtg catcagcagg aatgcgatcc ccgctctcta
     4981 aaaccaaaat atctccggga accaattcct tggcaggaat gcgtaccact ttggagttgc
     5041 gtaatacgtg cgccagcgga gcagttaact ctttcagtgc tgctaaagag cgttcagccc
     5101 gatattcctg gatgaaaccc aggatggcgt tgagaatgat aatggccagg atcgttaagg
     5161 cgtccgcccg ttcacccaat aagaatgaga caatcgcagc cccgatcaag accagcacca
     5221 tgaaatccgt gaactgttca cccaggatct gccacgcaga atctttctta atggtctgca
     5281 attgattcgg ccctgttcgg accaagcgtt tttgagcctc ggttgtagtt aaaccacgct
     5341 taagatcggc cccaaatatg cgggcaactt cggaggcctc tattccaaaa caattaccca
     5401 tgattttcgc ctttgacctc cccattctcc attgtatcag tggatcttat gcctgaataa
     5461 atagcggcag accattgact tgccgcaacc gtaaggctat actaaaagca ttgtcatccc
     5521 atatttcacg cttgtttgcg tgttaaggag gttcaccaaa aatgcctatc gacgcaccgg
     5581 cgattggctg tttatcccgg gagcttaagc attatttacc cgtcaagatc gataagattt
     5641 ggcaaccgta tcccgacgag tttatcttct catgtttcgg ttcgggcgcc tcctttaagc
     5701 tgttactctc ggttcatccc cagttcggcc ggtttcatct cttctctggc acccgtgaaa
     5761 atccgatcaa cccttccgcc ttttgtatgc tgttgcggaa acatttgggc ggcgcaaagc
     5821 tggttaccgt agatgccgtt ccatttga
//
LOCUS       DASVMB010000212         5993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_393799,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000212 DASVMB010000000
VERSION     DASVMB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393799"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="der"
                     /locus_tag="VF531_06365"
     CDS             complement(<1..657)
                     /gene="der"
                     /locus_tag="VF531_06365"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7713627.1"
                     /translation="MSQVVIAIVGRPNVGKSTLFNRITGQRLAIVEDTPGVTRDRLYA
                     NCHWLRREFLLVDTGGITDERDPVQTQIRKQVDLAVTEADGVIMVVDGRTPLTSADHQ
                     VADLLRKAHKPTVLAVNKVDNFTSTVEPEIYSLGLGEPIPISAEHGKNIGDLLDVLLA
                     TVPEAEQEAEADAIRVTMVGRPNVGKSSLVNALLGEERVIVSNEPGTTRDAIDTGLTL
                     N"
     gene            complement(723..2069)
                     /locus_tag="VF531_06370"
     CDS             complement(723..2069)
                     /locus_tag="VF531_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX7713628.1"
                     /translation="MEQNGAVIQSVEPESIAEKSGLRAGDILEYINGSPVRDLIDYLF
                     LSADEDLNLEVLQKSGTRREIRIEKTADEPLGICFASELFDGIRSCANHCLFCFVHQL
                     PKGQRASLYIQDDDYRLSFLQGSYITLTNLTAADWSRIQDLHLSPLYISVHATDPLVR
                     QKLLGTKAAGPILRQMRRLARVGITMHAQAVLCPHINDGPVLVKTIRDLEKLWPAVAS
                     LAIVPVGLTSWRRNLFNLETFTKEEARSVLEIIRTAQDRNLNTRGTRFVYAADEWYLL
                     ADSPMPEHEAYEDYYQLDNGVGLIRWFEQDFIEVFPQLLPRLKKQQRHLIIVTGVAAE
                     PLWRKIQAIFGAECPGLKLEILPVVNDFFGRTVTVTGLLTGGDIAKAIREFHPMPEAV
                     FLIPGITLRDTGDLFLDGMAVSDLKSSCEPHHLEVTPTRAREWLEWLAEFLESRAA"
     gene            complement(2145..2582)
                     /locus_tag="VF531_06375"
     CDS             complement(2145..2582)
                     /locus_tag="VF531_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713629.1"
                     /translation="MKTKKLLIGAFENVSDAKNALGRIKKATWNRADLALIVPRKSSP
                     DVVQGIDFELASESFLDLPRKDFSPLWPGLRQESLAGVGEVLIGQGRSHEPEPLSLDL
                     SQPELEMIKQPLKQHKIITVLKANPEDLPKLRLIMETDGAELL"
     gene            2913..3245
                     /locus_tag="VF531_06380"
     CDS             2913..3245
                     /locus_tag="VF531_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7713630.1"
                     /translation="MDWLRTIVRFVVAALVLMFIGAIVPGFKSLGFVSALLAAIVIAG
                     ISYLIELVLGRQLSPYAHGVVGFLVSAVVIYITQFIVPGMSVSIIGALLASLVIGLID
                     LFIPTKVK"
     gene            complement(3323..>5993)
                     /locus_tag="VF531_06385"
     CDS             complement(3323..>5993)
                     /locus_tag="VF531_06385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEX7713631.1"
                     /translation="KSYKLNAVAKGLGIELLNHHRAVDDAQCAAGILLKAMEEVEKRN
                     LTRLADLNSLVSESSVEHLKTYHISILVQSPEGISHLYRLISDSHIRYYHRHPRIPKS
                     ELTRLRTGLLIGSACESGELYQAILDGVPEEKLFEIAGFYDYFEIMPLANNQFLVDTG
                     RVKSIEELKATNKLICDIGAKLGKPVVATGDVHFLKPHEEIFRRILQIGQGYEGADHQ
                     APLYLRTTEEMLAEFDYLGPELAQEVVVTNTNLILERCGDVKPIPDEFCPPVIPGAEE
                     EIREMSYKKARELYGDPLPKLVEERLEWELKNIIGHGYAVLYLTAQKLVKKSLDDGYL
                     VGSRGSVGSSFVATMCGITEVNPLPAHYRCSKCKYSEFKETGSIGSGFDLPDQDCPKC
                     GTRMIKDGQDIPFATFMGFEGDKEPDIDLNFSGEYQPVVHRYTEELFGKGQVFRAGTI
                     TGLAEKMAFGFVKGYMEEKQVKLRQSEINRLVQGCTGVRRSTGQHPGGMYVVPQGRAI
                     YEFTPIQYPANDKKAEWITTHFEYHGGLEGRLVKLDILGHDDPTVIRMLNDITGIDPR
                     TVPLDDPATMKIFSTVESLGITAEALGFDLGTLGIPEFGTGFVRQMLEDTRPTTFAEL
                     LFISGLSHGTSVWLGNAQDLIKNGTATLSQVISTRDDIMNYLIFKGLPPKMSFKIMEK
                     VRKGKGLDEDDIKLMKEHDVPDWYIDSCQKIKYMFPKAHAAAYVISAFRIAYFKVHYP
                     EAFYATYFSVRADEFDADIVVQGEKPVRETRDAIRHKGNEATQKEKNLETILDMVLEA
                     ILRGIKFLRVDIYKSDPRRFLITPEGLLPPLASLQGLGENAANYLAAAREKEFVSIED
                     LKTRARLSSAVIEVLQKHGCLAGLSATDQLTLF"
BASE COUNT         1432 a         1547 c         1463 g         1551 t
ORIGIN      
        1 gttcaatgtt aacccggtat cgatggcgtc acgcgtagtc ccgggttcgt tgcttacgat
       61 cacccgttct tcaccaagta aggcattgac cagggatgat ttgcctacat tgggccggcc
      121 caccattgtt acacggatcg catcggcctc cgcttcctgt tcagcctcag gcactgtggc
      181 cagcaaaaca tcgagtaaat cgccgatatt ctttccgtgc tccgcggaga tggggatcgg
      241 ttcccccagg ccaaggctgt aaatctccgg ttccaccgta gaggtgaaat tatctacttt
      301 gttaacggcc agaactgtag gtttatgggc cttacgcaac aaatccgcga cctgatggtc
      361 ggcactggtc aaaggggtcc gcccgtcaac aaccataatc actccgtccg cttcggtgac
      421 agcaaggtct acctgtttac ggatctgggt ctgaaccgga tcccgctcgt ccgtaatgcc
      481 tccggtatcc accaataaaa actcccgccg aagccagtgg cagttggcgt aaagccggtc
      541 ccgggttaca cccggagtat cttctacgat agccaatcgt tgcccggtga tccggttaaa
      601 cagagttgat ttacctacat tgggccggcc tacaatggcg attactactt gtgacactcg
      661 ctacactccc tatcgataac tcagaacgaa taaaccaaaa gtcggttaca taaatcaaaa
      721 cgttatgccg ctcttgattc taaaaattca gccaaccatt ccagccattc cctggcgcgg
      781 gtaggagtta cctccaggtg atggggctcg cagctggatt tcaggtcgga taccgccatt
      841 ccgtccaaaa agagatcacc cgtatcccgt agtgtgattc ccgggattaa aaatactgct
      901 tccggcatag gatgaaactc ccggatcgct tttgcgatgt cgccgccggt aagcaaaccg
      961 gtgacagtca ccgtgcggcc gaaaaaatca ttcaccaccg gaagaatctc cagtttcaag
     1021 ccggggcatt ctgcgccaaa gatggcttga atcttacgcc ataacggttc agcggcaacc
     1081 ccggtgacaa tgatcagatg tcgctgttgt ttttttaatc tcggcagcaa ctggggaaaa
     1141 acttcgataa aatcctgttc aaaccaacgg attaaaccaa cgccgttatc gagttgataa
     1201 taatcttcat aggcttcatg ttccggcata gggctgtcgg ccaagagata ccactcatca
     1261 gcggcataaa cgaaccgtgt gccgcgggta ttaaggttcc tgtcctgggc ggtgcgaata
     1321 atttccaaaa cggatcgggc ttcttctttg gtaaacgttt ctaagttaaa taagttccgg
     1381 cgccagctgg tcaagcctac cggaactatg gctaagcttg ctacggccgg ccataacttt
     1441 tcaagatcac gaatggtctt gactagcacc ggaccgtcgt tgatgtgggg acataaaacc
     1501 gcctgggcgt gcatggtgat tccgacccgg gcaagccggc gcatttgcct taaaataggc
     1561 ccggctgcct tggtacccag caatttttgg cgaaccagcg gatcggtggc atggaccgat
     1621 atataaagag ggctcaaatg cagatcctga atccgggacc agtcggctgc ggtcagattg
     1681 gtcaaggtga tataacttcc ctgcaaaaaa gagagccggt aatcatcgtc ctgtatatat
     1741 aggctggcgc gctgcccctt tggaagctga tggacaaagc aaaaaaggca gtgattggcg
     1801 caagaacgga tcccatcgaa taattccgac gcgaagcata tccctaaggg ctcatcagcg
     1861 gttttttcta tcctgatctc gcgccgggtg ccggattttt gaaggacttc cagattcaaa
     1921 tcctcatccg cactgagaaa caaataatca attaggtccc gtaccggaga accattgata
     1981 tactccagaa tatcaccggc ccgaaggcct gacttctctg caatactctc cggttcaacg
     2041 gattgaatca ctgctccatt ttgttccaat aatcttctcc tttcggcaag attggtaagt
     2101 attcgcgtta atccaaacaa ctcctgcttg acaaggaact gtttttatag caattccgcc
     2161 ccgtccgttt ccatgatcaa ccggagtttc ggtaaatcct ccgggttcgc tttaagaact
     2221 gtaatgatct tatgttgctt tagaggttgt ttaatcattt cgagctcggg ttgagacaga
     2281 tcgaggctta agggctcggg ttcatggctc cggccctggc caattaatac ttctccgact
     2341 ccggcgaggg actcctgacg taatcccggc cataatggcg aaaaatcctt gcggggcaga
     2401 tccagaaaag attcggaggc cagttcaaaa tcgatcccct gtaccacatc cggggatgac
     2461 ttcctgggaa cgattaaggc aagatccgcc cggttccaag tggctttttt tatccgtccc
     2521 aatgcgtttt tggcatcgct tacattttca aaggctccaa tcagcaactt tttcgttttc
     2581 atccatttca cctcgtttta gtaccctcct tccaagacgg gaccgtttgt aagcactggc
     2641 cggtcgtttg agtttgaatc gggtcgacgg ttggtggcca aatagcgttc caaactgggt
     2701 tccgtccggg agtcggtcta gatagtttcc cacaaaagct ccggttttag taaagttgtg
     2761 gctttcccaa ggaaaatctg gattatcgca ttttcataag agtgacatgg attgcgtctc
     2821 aagcccttcg ccctttatcc gttttgaata ttcttttttc ccttgggaaa tactagtgat
     2881 aaagacttgg gctgtgcaaa gggggtgaat tcatggattg gttgaggaca atcgttcgat
     2941 ttgtagtggc ggctcttgtt ttgatgttca tcggagccat tgtacccggc tttaaatcgc
     3001 taggtttcgt cagtgctttg ctggcagcga tcgttatcgc cgggatcagt tatctgattg
     3061 aactggtatt aggcaggcaa ttatccccat acgcccatgg agtggtagga tttttggtat
     3121 ccgctgtagt gatttacata acccaattca tcgtgccggg gatgtccgtt tccataatcg
     3181 gagccttatt ggcatcgctg gtgatcgggt tgatcgatct ttttattccc accaaggtca
     3241 aataactcac gccataaata tcaagggtag ccgatggatt gagaaacggc tacccttggt
     3301 ttcaatcgat gcttttttat gcttaaaaca aagtgagttg atccgtggct gaaagcccgg
     3361 caagacaacc gtgcttttgc aggacctcaa tcaccgccga gcttaaacgg gctcgagtct
     3421 tgagatcctc gatcgagaca aactctttct ccctggctgc agccaaataa ttggcggcat
     3481 tctcccccaa gccctgtagt gaagccagcg gaggcagtaa gccctccggg gtgatcaaaa
     3541 aacgtctggg atcggatttg taaatatcca ccctcaaaaa ttttattccc cgaagtattg
     3601 cctcaagaac catgtccagg atggtttcca gattcttctc cttttgggtg gcttcattcc
     3661 ccttatggcg gatggcatcc cgggtttctc gaacgggctt ttccccctga accacgatat
     3721 ccgcatcaaa ttcatcggca cggactgaaa aatatgtggc gtaaaaagcc tccggataat
     3781 gaactttgaa gtaagcaatc cggaaggcag agattacgta ggctgcggca tgggccttgg
     3841 gaaacatata tttaatcttc tggcaggagt caatgtacca gtcgggtaca tcgtgctctt
     3901 tcatgagctt aatgtcgtct tcgtccaggc ctttgccttt acggaccttt tccatgattt
     3961 taaacgacat cttgggtggc aagcctttaa agattagata gttcatgata tcatcacggg
     4021 tcgagattac ctgggatagt gtagcggttc cattcttgat taagtcttgg gcgttaccca
     4081 gccaaacgct ggtgccgtga gaaagcccgg aaataaacag taattccgca aaggttgtcg
     4141 gccgggtatc ctccagcatt tgccggacaa aacccgtgcc aaactccgga attcccagag
     4201 tacccagatc gaaaccgagt gcttcggcgg taatacctaa cgactctaca gttgaaaaaa
     4261 ttttcatggt cgccgggtca tctaacggca cagtccgggg gtcgataccg gtgatgtcgt
     4321 tcagcatccg gatcaccgtg ggatcatcat ggccgaggat atccagttta accaaccggc
     4381 cttcaagacc accatgatat tcaaagtgag tcgtaatcca ctcggctttc ttatcattgg
     4441 ccggatattg gatcggtgtg aactcgtata tcgcccgtcc ttgcggcacg acatacatac
     4501 ccccgggatg ttggcccgtg gaacggcgta ccccggtgca accctgaacc agccggttaa
     4561 tctccgattg acggagttta acctgttttt cttccatata ccctttgacg aaaccgaaag
     4621 ccatcttttc agccagtccg gtgatggttc cggcccggaa tacctggccc ttgccgaaga
     4681 gttcctcggt gtaccgatgc accaccggtt ggtactcacc ggagaaattt agatcgatat
     4741 cgggttcctt atcgccttca aaccccataa aggtcgcaaa gggaatatcc tgcccgtcct
     4801 tgatcatcct ggtgccacat ttggggcaat cttgatcggg aaggtcaaag ccggaaccaa
     4861 tggagccggt ctctttaaac tcgctatatt tacatttaga gcaacggtaa tgggcaggga
     4921 gtggattgac ttcggtaata ccgcacattg tcgcaacaaa ggatgagccc accgaccccc
     4981 gggagcctac cagatatccg tcgtccagcg acttcttcac cagtttctga gctgtcaaat
     5041 ataaaacggc ataaccgtgt ccgataatgt ttttgagctc ccattccagc ctctcctcga
     5101 ccagtttggg tagaggatcc ccatataact cccgggcctt tttgtaagac atctcgcgga
     5161 tctcttcttc cgctccggga attaccggag ggcaaaactc gtcgggaatt ggtttaacat
     5221 cgccgcagcg ttccaaaata aggttggtgt tggttaccac cacctcttga gcaagctccg
     5281 gcccaaggta gtcaaactcc gccagcatct cctcggtagt gcgtagatat aacggagctt
     5341 gatggtctgc tccttcatag ccctgcccaa tttggagtat ccgtcggaag atctcctcgt
     5401 ggggttttag aaaatggaca tccccggtgg ccaccaccgg tttaccgagt ttggcgccga
     5461 tatcacagat caatttatta gtggccttga gctcttcgat tgatttgacc cggccggtat
     5521 cgactaaaaa ctggttgttg gcaaggggca tgatctcgaa ataatcatag aaaccggcaa
     5581 tctcaaataa tttctcctca ggcaccccgt ccaggattgc ctggtaaagc tcccccgact
     5641 cgcaggccga cccgatgagt aacccggtcc gtaaccgggt taattcggat ttgggaatcc
     5701 ggggatggcg gtgatagtag cggatatggg aatcggagat caagcggtag agatggctga
     5761 ttccctctgg agactgaacc agaatcgaga tgtgataggt tttaagatgc tctacactgc
     5821 tctcggagac gagggaattt aaatccgcta gccgggttag attccgtttc tcaacttcct
     5881 ccattgcctt aagcaggata ccggctgcgc actgcgcgtc atccactgcc cggtggtggt
     5941 tgagaagctc gatccctaat cccttggcta ctgcattcag tttatagctt tta
//
LOCUS       DASVMB010000213         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_26067,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000213 DASVMB010000000
VERSION     DASVMB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26067"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /locus_tag="VF531_06390"
     CDS             <1..1889
                     /locus_tag="VF531_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX7713632.1"
                     /translation="FTELPLGAIKPEGWLRNQLSIQAAGLTGHLDEFWDSVGSYSGWL
                     GGTGENWERGPYYCDGLLPLAYLLDDEALIAKTRPWIEWTIGSQRPDGWFGPAANEDW
                     WPRMVMLKVLVQYQEVTADSRVIPFMSRYFRYMKDRIDEVPLRDWGRARGGEYLYSLY
                     WLYNRTGEAFLLELAETIFRQTLDWTGIFTGFPFVKPTDFYYNWQKIARHNWDEINNL
                     MQYHATHVVNVVMGIKEPGLYYQQSRDPRHREAVFKGIAALARYHGQVAGNVAGDEHL
                     SGTNPSRGSELCSVVEYMFSLETQLKMASNSRLGDLLEKIAYNALPAAFGKDMWSHQY
                     DQQPNQVLVTKAKRDWFNNNEEANLFGLEPHFGCCTANMHQGWPKLVEHFWLATADRG
                     LAAMVYGPCRVEAEVGSGVTVRIDEITNYPFNGRIEFRLHCPVKIKFPLMLRIPAWCQ
                     GAEVKINGAVFNRPDAGGFRIIDREWQDQDQISLELTFEVRTVSRYRDSVALERGPLV
                     FALRIGEEWRQLKGIQPHADWEVYPTTPWNYALAVDSDHPEGSVRVLCNDLKYQPFDP
                     ENSPVELRITGRRLPDWKLEANSAGELPQSPVVSGEPDEELVLVPYGAAKLRITEFPR
                     LKGE"
     gene            2161..3444
                     /locus_tag="VF531_06395"
     CDS             2161..3444
                     /locus_tag="VF531_06395"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713633.1"
                     /translation="MNSMQRRNLSLFISGRLISLIGSGIQMIAIPLYILDLTGSGTLM
                     GVFTMLSMVPMLLISPLAGVLGDRLSRKSIAVYTDLGRGALIMVLSWLALNGWMSIPA
                     LFASQILISIMDSLFNAATAAMLADLVDVADFNRANASKSAIDSLSMIVGPVIGGIIY
                     GTSGITMVFFLNAASFLISGLCEIFITYRATIQNGIKLTARSFMTEIRDVLSFINRHT
                     GLKQLFAFAMVSNFLASPLIMVAFPYIFKKTIGFTSEQYGYLMTTFMVGLLIGNILIG
                     SIFSKSGAGGLMRTGLVAQGFLLMILAAITFPVVVQGFQGPSWKFFGVIAGGFISSGI
                     FNALVNTPLNTNLQKMVPPEMRSRFFAVLGLIAQLAVPVGSVIYGFLLDMIPSHLIMA
                     IVGFINFVITLVFIKYASPEAYEPKAYTPDLSVTD"
     gene            3468..>3659
                     /locus_tag="VF531_06400"
     CDS             3468..>3659
                     /locus_tag="VF531_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HEX7713634.1"
                     /translation="MRYKAPGQCPVCNHELIVTQLNCTFCSTKLEGEFTSCKFCRLPA
                     EQRDFIEVFIKCRGNIKDVE"
BASE COUNT          820 a          864 c          976 g          999 t
ORIGIN      
        1 cctttacgga gcttccgctg ggagcgatca aaccggaagg ttggttgcgc aatcaattat
       61 cgattcaagc ggccggcctc accggccatt tggatgagtt ttgggattcg gtgggatcct
      121 acagcggttg gctgggaggg accggtgaaa attgggaacg gggcccgtat tattgtgatg
      181 gtttattgcc tttggcgtat ctgttggatg atgaggcttt gattgccaag acccgtcctt
      241 ggattgaatg gaccataggc agtcaaaggc cggacggttg gtttggtccg gctgccaacg
      301 aagattggtg gccgcggatg gttatgttga aggttctcgt ccaataccag gaagttaccg
      361 cggattcccg ggttataccg tttatgagcc gatactttcg ttatatgaag gaccggatcg
      421 atgaagtccc attacgggac tggggccggg cccgtggtgg cgaatatttg tatagcctgt
      481 actggcttta taaccggacc ggggaggcat ttctcctgga actggcggag acgatctttc
      541 gccagacgtt ggactggacc gggatattta cggggtttcc ctttgtcaag ccgaccgatt
      601 tttattataa ctggcaaaag atcgcccggc ataactggga cgagatcaat aatctgatgc
      661 aatatcatgc cactcatgtc gtcaatgtag tgatgggcat caaggaaccg gggctttatt
      721 atcagcagtc ccgggatcct cgccatcgtg aggcggtttt taagggaatc gccgccttgg
      781 cccgttatca tggtcaggtt gcgggaaatg tggccggaga tgagcatttg agcggtacca
      841 atcccagccg cggctcggaa ctatgttccg tagtggagta tatgttcagt ttggaaacgc
      901 aactcaagat ggcttccaat tcaagactcg gcgatttact ggagaagata gcctataacg
      961 ccttgcccgc ggcattcggc aaggatatgt ggagccatca gtacgaccag caacccaacc
     1021 aggtgctggt gaccaaggcc aagcgcgact ggttcaataa taacgaggag gctaacctat
     1081 tcggactgga gcctcatttt ggatgctgca ccgccaatat gcaccagggt tggcccaagt
     1141 tggtggagca cttctggctc gccacagccg accggggttt ggcggcgatg gtttacggac
     1201 cttgccgggt tgaggcggag gttggttcgg gagtgaccgt tcgcattgat gagattacga
     1261 attatccttt taacggccgg atcgaattcc ggctccattg cccggtaaag attaagttcc
     1321 ccctgatgct ccggattccc gcctggtgtc aaggggctga agtaaagatt aacggagccg
     1381 tatttaaccg accggatgca ggaggcttcc gtatcatcga tcgggagtgg caggatcagg
     1441 atcaaatttc actcgaattg acctttgagg tcaggacagt ctcccgctac cgggactccg
     1501 tggcgctcga acgcgggcca ctggtgttcg ccctgcgaat cggggaagaa tggcgtcaac
     1561 tgaaaggcat ccagcctcat gccgattggg aggtttatcc gaccacccct tggaattacg
     1621 cactggccgt cgactccgat catccggagg gttcggtgcg ggttttatgc aatgatctga
     1681 agtatcagcc ttttgatccg gagaattccc cggtggagtt acgtattacc ggccgccgtt
     1741 tgccggactg gaaactggaa gccaattccg ccggagaact tccccaaagc ccggtggtct
     1801 ccggtgaacc ggacgaagag ttggtgctgg taccctatgg agccgcaaaa ctgcggatca
     1861 ccgagtttcc caggttaaaa ggggaatgaa tttgtcttac tcttggcttc ccggtcccaa
     1921 ccggagaaga ccaatttaac cactcacctt tttattgccg cgcctgtctt tggcgcggtc
     1981 tttttttatt gcgtagatta ttcacctggt tttcgccacg gccggatgat ttgggttaaa
     2041 cacgggatta ttaattaaat taaacatgaa cttaatatta ttaaagattc aattgacaaa
     2101 attaatttgt gatggtagac taaggattac aattcagtac tcacacagga gtcgattcaa
     2161 atgaattcta tgcaaaggcg aaatttatcg ttgtttatct ccggccgctt gatatcgctt
     2221 atcggcagcg gtatccaaat gattgccatt cccttataca tcttggatct taccggttcc
     2281 ggaaccttaa tgggtgtgtt caccatgctc agcatggttc cgatgttact catttccccg
     2341 ctcgccggag tcctcggtga tcggcttagc cggaaaagca tcgcggtata tacagatctg
     2401 ggcagagggg ccttgatcat ggttctttcc tggttggctc ttaatggttg gatgagcata
     2461 ccagctcttt ttgccagtca aatattaatt tctatcatgg atagcctctt taatgccgca
     2521 actgctgcca tgttggccga tctggtggat gtggcggatt tcaaccgtgc caatgcttcg
     2581 aaaagcgcta tcgatagttt gtcgatgatt gtcggaccgg ttataggcgg gattatttac
     2641 ggcacatccg gaattacaat ggtctttttc ctcaatgccg catcctttct catctcgggg
     2701 ctgtgtgaaa tattcattac ttatcgcgcc acgattcaaa atggcatcaa attgactgca
     2761 cgttctttca tgacggagat ccgtgatgtc ctttccttca tcaaccggca taccgggttg
     2821 aaacagttat tcgcctttgc catggtttcg aactttttgg cctctcccct gatcatggtc
     2881 gcttttccat atatttttaa gaagaccatc ggatttacct cggaacaata cgggtatcta
     2941 atgactactt tcatggtggg gttattgatt ggtaatatcc tgatcggctc cattttctcc
     3001 aaatcgggtg caggtgggtt gatgaggacc gggctagtgg cccaaggatt tttactcatg
     3061 attttagccg caataacttt tcccgtagtt gtccaaggat ttcaagggcc gtcctggaaa
     3121 tttttcggag ttattgccgg tggcttcatc agcagcggaa tttttaacgc gttggtcaat
     3181 acgccgttaa ataccaatct gcagaagatg gtccctccgg agatgcgatc ccggttcttc
     3241 gcggttttag ggctcatcgc ccagctggcc gtgccggtgg ggtcggtcat atacggcttc
     3301 ttgcttgata tgatcccgtc tcatctgatc atggcgattg tcggctttat caattttgtg
     3361 attacgcttg tctttatcaa atacgcctca cccgaggcat acgaaccgaa ggcttatacg
     3421 ccggatcttt ccgtaaccga ttaaggaatg aagagaggaa ttcaaccatg cgctacaaag
     3481 cacccggtca atgcccggta tgtaatcacg aattgattgt tacccaatta aactgcacct
     3541 tttgctcaac caagctggaa ggggagttca catcctgtaa gttttgccgg cttcccgcgg
     3601 aacaacggga tttcatcgag gtattcatca agtgccgcgg caatatcaag gatgtcgag
//
LOCUS       DASVMB010000214         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_26308,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000214 DASVMB010000000
VERSION     DASVMB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26308"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..749)
                     /locus_tag="VF531_06405"
     CDS             complement(114..749)
                     /locus_tag="VF531_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7713635.1"
                     /translation="MGIAERKRIEKEELQRKILDAAGTILIEEGYRSLSIRKIAARIE
                     YSPGLIYHYFKDKAAIVTALVEQGYGRILQAIGTVPADPADPVGTIRRTLIRYIELVL
                     ESPLEYRAILLNDIEEVREKVGILDRGISKRRKSMELLGGIIARAVQEAQFRPVEPEL
                     AAQIIWTSTYGLLARLLLEPEIPPEQRERLLNQHLDILFYGLVARERVETK"
     gene            complement(978..1208)
                     /locus_tag="VF531_06410"
     CDS             complement(978..1208)
                     /locus_tag="VF531_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HEX7713636.1"
                     /translation="MRASELEGKEVIDIHTGDRLGVIQKTELLINTVTGTVEGLIMVK
                     RGFGGREKEFRTIPWREVKKIGDELIMVESED"
     gene            1584..2060
                     /locus_tag="VF531_06415"
     CDS             1584..2060
                     /locus_tag="VF531_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX7713637.1"
                     /translation="MAIAEIRGGPLAPEIQGTVLFQDLPGGTMISVYVTGLPAYQPAT
                     SEQAPIGPFGFHIHEFGNCIVGDPQNPFQAAGGHWNPTREPHGNHAGDLPVLFSNHGM
                     AQFNFFTDRFQVKDLLDRTVIIHQNPDDYRSQPAGASGKRLACGVINLCSRVREGH"
     gene            complement(2256..3269)
                     /gene="gap"
                     /locus_tag="VF531_06420"
     CDS             complement(2256..3269)
                     /gene="gap"
                     /locus_tag="VF531_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7713638.1"
                     /translation="MAVKVGINGFGRIGRLVFGASLDNPAVEVVGINDPFIDLEYMTY
                     MLRYDTVHGQFKGTIGTEGTDILIVNGKKIKVFAVKEPSEIPWQSCGADYVVESTGLF
                     TTIEKASAHFKGGAKKVIISAPSSDAPMFVMGVNQDKYTKDMKVVSNASCTTNCLAPL
                     AKVIHENFGIVEGLMTTVHSTTATQKTVDGPSKKDWRGGRAAAANIIPSSTGAAKAVG
                     KVIPELNGKLTGMSFRVPTIDVSVVDLTCRLEKAATYDEIKAAVKKASENELKGILGY
                     TEDDVVSSDFIHDPRTSVFDAKAGIALNDHFVKLVSWYDNEWGYSNKVVDLICHMGKV
                     DAK"
     gene            complement(3398..6013)
                     /gene="clpB"
                     /locus_tag="VF531_06425"
     CDS             complement(3398..6013)
                     /gene="clpB"
                     /locus_tag="VF531_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX7713639.1"
                     /translation="MDVNRMTQKVQEALAAAQSMAVRYGHQQVDVEHLCLALLEQEAG
                     LAISIINRADADARSVAERVKRELERLPRVTVEGPGAAADQIYITGRLNRILAQAETE
                     AKRLKDEYISVEHLLLALVDDGGATGRIFKELRLSRERLSHALQEIRGNQRVTSPNPE
                     ATYEALERYGRDLTKFASEGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKT
                     AIVEGLAQRIFRGDIPEGLKHKRVVSLDMGALIAGAKYRGEFEERLKAVLKEVQESAG
                     QIILFIDELHTVVGAGKAEGAMDAGNLLKPMLARGELHCIGATTLDEYHKHVEKDAAL
                     ERRFQTVLVDQPSVEDTISILRGLKERYEIHHGVRIKDAALVSAAVLSNRYISDRFLP
                     DKAIDLVDEAAAKLRTEIDSMPTELDELLRRVMQMEIEREALKKETDPASRERLERLV
                     RELAEYKGEADALKARWEKEKQGVQRLRKIREDIEATKAEAEQAELQYDLNRAAELKY
                     GKLTGLERQLEGEQEKLSLGEDGPRMLKEEVSEEDIAEVVSRWTGIPVSKLLEGEAQK
                     LLHLEEELHRMVIGQDEAVRAVAEAVIRARSGLKDPNRPIGSFIFLGPTGVGKTELAR
                     ALAWFLLDDPGNMIRIDMSEYQEKHTVARLIGAPPGYVGYDEGGQLTEAVRRRPYAVI
                     LFDEIEKAHYDIFNLLLQILDDGRLTDGQGRTVDFRNTVIIMTSNIGSQRILDYQGGF
                     NGENYERMKDAVLGEMRHQFRPEFLNRIDEIIVFHSLTEEHLKQIVTIRLEYLEKLLA
                     ERHIGLELSDAAKSFLVQVGYEPSYGARPLKRAIQKELETPLGRLILSGEIKDGERVQ
                     VDYSEGRGLTFCGVE"
BASE COUNT         1491 a         1879 c         1429 g         1643 t
ORIGIN      
        1 agcctttctt gccgctgttg cagtttgagc catgatactc attcctatcc ctccaagtat
       61 gaataatgcc aaaagaaatc cggtcacccg ccggtatggt ttcctaatca acatcatttc
      121 gtctccaccc tctcccgggc gaccagcccg taaaataata tatcaagatg ctggttgagc
      181 agtcgttccc tttgttccgg cggaatctcc ggctccagta acaatcgggc caaaagcccg
      241 tatgtcgaag tccagatgat ttgtgccgcc agctccggtt caaccggacg gaactgcgct
      301 tcttgaaccg cccgggcgat aatcccgcca agcaactcca tactcttccg tctttttgaa
      361 attccccggt ccagtatacc gactttctcc ctgacctctt cgatatcatt gagtaaaatc
      421 gcacggtatt ccagaggcga ctctaaaacc aattcaatgt aacggataag ggtacggcgg
      481 attgtcccta ccgggtccgc cggatctgcc ggaaccgtgc cgatagcctg gagaatccgg
      541 ccataacctt gttccaccaa ggccgtcact atcgctgcct tgtccttgaa ataatgatag
      601 atcaatcccg gggaatactc gatccgggcg gcgattttcc gtatagaaag gctcctgtat
      661 ccttcctcga tcaaaatcgt accggcggca tcgagaatct tccgctgcaa ctcttccttt
      721 tcgatccgtt ttcgttccgc aatccccact tgtggtcacc ctttacctca tcggtttaaa
      781 ccctttaccc ccgaataacg gttgaattca tatcaacccc gtacccaaac atagattcat
      841 aaattgactc caaggccaat ggttcttttg tatcaaggcg cagagatagg taaacaccgt
      901 atattctttg aacatcgttt aatgaaattt tagcatactc ccattcccta gacaagttaa
      961 cttttggata tctcgcatta atcttcagat tcaaccatga ttaactcatc gccgatcttc
     1021 ttgacttctc gccagggaat ggtccggaac tctttctccc tgcccccgaa gccccgtttg
     1081 accatgatca ggccttcaac cgtaccggtg accgtattga tcaagagttc ggtcttttgg
     1141 atcaccccca gccggtcccc ggtgtgaata tcaataactt ctttcccctc aagctcactg
     1201 gcgcgcatcc cgaacccctc ccctgccgca tttttaacga attttgccaa tggttaaaga
     1261 tatgtggctt ttactggatt tagagccgaa aatcattgct taacaatcaa agattccaaa
     1321 ccatatcggc tccttaggtt cctcttttca aaacaactct catagaatat accaaagatt
     1381 ctactcgcga aattgtacgg ttttcttgct tccgatagat tcctggttga acggttttca
     1441 aaatttaccg aaaaggggtg cctcgtatga agaaaggaga ttcttccccg gaccgttccc
     1501 gacagtcgga agttcatacg actgccttcc ccggtaagct cattccgggt tggacctggt
     1561 taccgcagcg caacgatgtt ccaatggcaa tcgccgaaat ccgcggcggt ccgctggccc
     1621 cggagattca ggggacagta ttatttcaag atcttcccgg tggtaccatg atttcagtct
     1681 acgtcaccgg tctcccggca taccagcctg ccaccagcga acaagcgccc atcgggccgt
     1741 ttggttttca tatccatgaa ttcggcaact gtatcgtcgg tgatccccag aacccgtttc
     1801 aggcagcggg agggcactgg aatcccaccc gtgagcctca cggcaatcat gccggagacc
     1861 tcccggtttt attctccaac catggcatgg cccagttcaa tttctttacc gaccggtttc
     1921 aggtgaagga tctgctcgac cggacggtga tcatccacca gaatcccgac gattatcgct
     1981 ctcagccagc cggtgcctcg ggaaaaagac tggcctgcgg cgtgatcaat ctctgctccc
     2041 gcgtacgaga gggccactag cttgaatcag cctaatggct gagggcattg agagcggagc
     2101 cgacagatat ttttctgtcg tgtgcgagta aacggaatag tcatataaca cttaattaaa
     2161 aacaaggggc atgcccgtaa ctaaccgggt agcccctgtt tcatcggtaa cgttcgcttc
     2221 accgatgttc atatcatttg cgtcgtgacg ctcaattact tcgcgtcgac ttttcccata
     2281 tggcagatga gatccaccac tttgttggaa taaccccatt cattatcata ccaggaaacc
     2341 agcttgacga aatggtcgtt caaagcgata ccggccttgg cgtcaaagac ggaggttctg
     2401 ggatcatgaa tgaagtcgga agataccaca tcatcctcgg tgtaaccgag aattcctttc
     2461 aactcgttct cggatgcctt tttcacagca gccttgatct catcgtaggt cgcggccttc
     2521 tcaagacggc aggtcagatc gaccacggat acatcaatgg tcggcacccg gaaggacata
     2581 cccgtcagtt taccatttag ctcgggaata actttcccga cagccttcgc ggctccggtc
     2641 gaggaaggaa tgatattggc cgcagcagcc cggccgcctc tccaatcctt cttggacggg
     2701 ccgtcaaccg ttttctgggt cgcggtggtc gagtgaacgg tagtcatcaa gccttcgacg
     2761 ataccgaagt tctcgtgaat gaccttggcc aaaggcgcca ggcagttggt ggtacaggaa
     2821 gcgttggaga caaccttcat gtctttggtg tatttatctt ggttgacacc catgacgaac
     2881 atcggggcat cgctggaagg agcactgata ataaccttct tggcgccgcc cttgaagtga
     2941 gccgatgcct tttcaatggt ggtgaataaa ccggtagact cgacaacata atccgcgccg
     3001 caagactgcc agggaatctc cgacggctct tttacggcaa aaactttgat ctttttcccg
     3061 ttgacaatca ggatgtccgt gccttcggtg ccgatggtcc ccttgaattg gccgtgcacg
     3121 gtatcatatc tcagcatgta ggtcatgtac tctaaatcga tgaacggatc gttgataccg
     3181 actacttcta ccgccgggtt atccagtgat gctccgaaga ccagacgtcc aatgcgtcca
     3241 aaaccgttaa taccaacttt tactgccatc aaagctaccc cctatttttt attattgtgg
     3301 atgatattaa accaaatttt acctttgcca taacaatttt ataaaaattc acgacctttt
     3361 tcaatagatc agccgataat gaatcaaaat attaacatta ttcaaccccg caaaatgtga
     3421 gaccccggcc ttcgctataa tccacttgaa cccgttctcc atccttgatt tcaccgctta
     3481 ggatcaaccg gcccagcgga gtttcaagtt ccttctggat tgcccgtttc aacggccggg
     3541 cgccgtagga tggctcataa cccacctgaa ccaagaaact cttggctgca tcgcttaact
     3601 ccagcccgat atggcgctcc gccaacaact tttccaggta ttccaggcga atggtaacga
     3661 tctgtttcag atgttcctcc gtgagagaat ggaagacgat gatctcgtcg attcggttaa
     3721 ggaactccgg acggaactga tgccgcatct ccccgaggac cgcgtccttc atccgttcgt
     3781 aattctcccc gttgaacccg ccctgataat ccagaatccg ttggctcccg atgttggaag
     3841 tcatgatgat cacggtgttc ctgaaatcaa ccgtccggcc ctgaccgtcg gtaagccgtc
     3901 cgtcatccag aatctgcagt aataaattga agatatcata atgagccttc tcgatctcgt
     3961 caaagagaat cactgcatag ggccggcgcc ggacagcctc ggtcaactga ccgccttcat
     4021 cataccccac ataaccggga ggagcgccga ttagtctggc caccgtatgt ttctcctgat
     4081 actccgacat atcgatccgt atcatattgc cgggatcatc cagcagaaac caggccaggg
     4141 ctctggccag ttcggttttg ccgacaccgg tcggaccgag aaaaataaag gaaccgatag
     4201 gccggttggg gtccttcaat ccggaacgag cccggatcac cgcctcagcc accgccctta
     4261 ccgcctcatc ttggccgata accatccggt gcagttcctc ttcaaggtgg agcaatttct
     4321 gggcctcgcc ctccagcaat ttcgaaacgg gaatcccggt ccacctgctg acaacttcgg
     4381 caatatcctc ctcggatacc tcttctttga gcatccgggg tccgtcttcc ccaagggata
     4441 acttttcctg ctccccttca agctgtcgtt ccagaccggt caacttgccg tactttaact
     4501 ccgcggcgcg gttcagatca tactgcaact ccgcttgttc ggcctcggcc ttggtggcct
     4561 cgatatcctc tcgaatcttg cggagccgtt ggactccttg tttctccttt tcccaacgag
     4621 cctttagggc gtcggcctcg cccttatact ccgcgagttc tctcacgagc cgttccagcc
     4681 gttcccggga agcggggtca gtctccttct ttagagcttc ccgctcgatc tccatttgca
     4741 ttacccgtcg taacagctcg tccagctcgg taggcattga atcgatttcg gtgcgtaatt
     4801 tggccgccgc ctcatccacc aggtcaatgg ccttgtccgg caaaaaacga tccgagatat
     4861 agcggttgga aagcacagcc gccgaaacca gcgccgcatc cttgatccgt acgccgtgat
     4921 ggatttcgta acgttccttt aaaccccgca aaatcgagat ggtatcttct accgagggtt
     4981 gatccaccag tacggtttga aaacgccgtt ccagcgcggc atccttctca acatgcttgt
     5041 gatattcgtc gagcgtagta gcaccgatgc aatgtaactc gccccgggcc agcatcggct
     5101 taagcagatt cccggcgtcc atggctcctt cggccttgcc ggctccaacc acagtatgca
     5161 gttcgtcaat gaacaaaatg atctggccgg ctgattcttg cacttctttc aggacagcct
     5221 tcaaccgttc ctcaaattcc ccccggtatt tggcgccggc gatcaaggcc cccatatcca
     5281 gggaaacaac gcgtttatgt ttcagcccct cggggatgtc accgcggaaa atgcgctggg
     5341 ccaagccctc gacaatcgcg gtttttccaa ctcccggctc cccgatgagc accggattat
     5401 tcttggttcg tcgcgacagc acctgaatga cacggcgaat ctcatcatcc cggccaatca
     5461 ccgggtccag cttaccttcg ctcgcgaact tggtcagatc tcggccgtac ctttccaagg
     5521 cttcataggt ggcttcgggg ttgggtgagg ttacccgctg gttgccgcgg atctcttgca
     5581 aagcgtggct gagtctttcg cgcgacagac ggagctcctt aaatatccgg ccggtagccc
     5641 cgccatcgtc caccaatgcc aggagcagat gttccacaga tatatactca tctttcagac
     5701 gcttggcctc ggtttcagcc tgtgccagaa tccggttgag cctcccggtg atataaatct
     5761 gatccgccgc tgcacccggt ccctccaccg taacccgggg caaccgttca agttcccgtt
     5821 ttacccgctc cgctacactc cgggcatctg catcggcccg gtttattatg gagattgcca
     5881 ggccggcttc ttgttccaat aaagccaaac aaagatgctc cacatccacc tgttggtgtc
     5941 cgtaacgtac cgccatgctt tgagccgcag ccagcgcttc ctgaaccttc tgagtcatac
     6001 ggttgacgtc catttcaatt tcctccagtt gaccaaactt gcaattgtta attataattt
     6061 aatattaata tagttaacta tacccataaa cccaatttcc cggtactttc gtgaatttca
     6121 tgttagaatt ctcaactgta ccagacagct aaaattggtg ggttattttt aaccacgaaa
     6181 cacacgaaag ccacgaaata aaatcagaag acaatgaccc tttttcgtga aatttagtgt
     6241 taaaatttct atattcaccc tgcaatctcg tacatgacag ggttacccca gagttggctc
     6301 tagaacttac ccactcattt caagatttaa tactaccaac gcaaccggat tggaaaaagt
     6361 tcctcagcta ccggatcggg ctgtagaaac accccgatcc ggccagcttc tcctttttat
     6421 tcaaagaatg cagatagctc cc
//
LOCUS       DASVMB010000215         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_172118,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000215 DASVMB010000000
VERSION     DASVMB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172118"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF531_06430"
     CDS             complement(<1..204)
                     /locus_tag="VF531_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713640.1"
                     /translation="MIELIVLLILGRKIGEVASDRGYGTKRGRYRLLLVLSWFGGEIA
                     GILIGVILSRSFFIYYPLGLVGAV"
     gene            454..1179
                     /locus_tag="VF531_06435"
     CDS             454..1179
                     /locus_tag="VF531_06435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7713641.1"
                     /translation="MVDTFLFDLDGTLLPLEMDEFIRIYFSEMGKALSDLIEPKRLAE
                     NIWAATRSMVANLEPKTNEAVFMEAFAGLIGGDLSVYRSRFDAFYEGPFLKAKAAVVD
                     QPMIRKSVALLKEKGYELVIATNPIFPKQAILRRIEWAGFSPEDFSYISSYEHNHYCK
                     PQLQFYQEVLADTGKKPEDCMMVGNDVEEDLIAGQLGIETFLITNYLIQKPGTNIVCD
                     CQGDYDAFYRFVTSLPEIGFKGA"
     gene            complement(1166..2170)
                     /locus_tag="VF531_06440"
     CDS             complement(1166..2170)
                     /locus_tag="VF531_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7713642.1"
                     /translation="MRNEGRKAGLGMGRRRDTVYSLPAESMNESVDREDSGSAANLLD
                     KIVLPQAVKLARSGHYPDAENLLLLLVQESGEQPEVYDLLARIQAQQGRYTEAEISWS
                     TALRLDPTNREYQLGLRRIRLQNGMAAERPLRGWLWGIGILLCFVMMGFSARHYLGEL
                     RTVRLPVPVFQRISTAPRRIPEETPRDRVQTRDLPQAFAKDLRIPGANVNYRKGQITV
                     VFNTGLFQRGVKLLPGAQASLKNLALQLRPYKNRVLVHVEGHTDNVPMPLGLIYRDNV
                     SLGMQRAVIVVDYLRAQADLPASMFTVSSLGEASLLYPNDSSLNQLRNRTVLIRITPL
                     "
     gene            complement(2167..2643)
                     /locus_tag="VF531_06445"
     CDS             complement(2167..2643)
                     /locus_tag="VF531_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713643.1"
                     /translation="MLMAGLRQLRYPWEFRIPPSETIDLEMVLRKIAWLAGDVSVQSA
                     GAESMQYLAEVGTGLWRLQRKMMEPGTDHPTPGMRRACRSMKEIWNDLARVGVMILDH
                     TDTIYTANMPLRVLSFEKVAGFTQARVIQTARPTIFLQERPIQMGEVVVGLPGEEE"
     gene            complement(2780..>3408)
                     /locus_tag="VF531_06450"
     CDS             complement(2780..>3408)
                     /locus_tag="VF531_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7713644.1"
                     /translation="PSSIGSSVSLGCFRMRNQDIAEVFGLVSVGTPVDIVYETVRPSI
                     DQQNQAWLELFPDIYHWGDQDAKVLNAIDNLAWNCQVHLRALAELIKDPKRPLKFPLP
                     RVIRIIETEPGPAETIEPGNGSNPDGFYWNGSIYLAKSVFTTWRVETANGVDPTFHDY
                     FNLQFYEGNNSGFYWKWDEANNTLRVGGTQEAAGRNLGALPGGRVSRG"
BASE COUNT          812 a          918 c          828 g          850 t
ORIGIN      
        1 aacggccccc accaaaccca gcggataata gatgaaaaaa ctccgactga ggatcactcc
       61 aatcaatata ccggcgatct cccctccgaa ccaggataag accaacagta atcgataccg
      121 gccccgcttc gtcccatagc cgcggtctga cgcgacctca ccgattttac gacccaaaat
      181 aagcagcaca ataagttcaa tcatattttg tctccccttt cgccacagat ccgatcttgc
      241 gtccaggcac gcaccattcc tctttggaag aaatgtttga gaaacatccc ttctaaagag
      301 ccatggagat cggctgttta caatttatgc ggcatccggg ggattcaacc tgcttgcacc
      361 ggtgtgataa tttatgttaa gattagcatt aatgaaatcc ggggagctgt taacaaacgt
      421 tgcagcgtac tcggagattt ggaggttttt gcgatggtgg acactttcct ttttgacctg
      481 gatggtacat tattgccact cgagatggat gagtttatca ggatttactt ttccgagatg
      541 ggtaaggctt tgagtgatct aatcgaaccc aagcgtctgg ccgagaatat ctgggcagcg
      601 acccgcagta tggtagccaa tctcgaaccg aagacgaatg aagccgtatt tatggaagcc
      661 tttgccgggt tgatcggtgg tgatctctcg gtttatcgga gccgtttcga tgcattctac
      721 gaaggtccat tcttaaaagc caaagctgcc gtagtggatc aaccgatgat ccggaaaagt
      781 gtcgcccttt taaaggaaaa aggttacgaa ttagtgatcg ccaccaaccc gatctttccc
      841 aagcaagcga ttttgcggcg aattgagtgg gctggatttt ctcccgagga cttttcttat
      901 atctcatctt atgaacataa tcattattgc aagccccaat tgcagtttta tcaggaagta
      961 ttggcggata cggggaaaaa gcctgaagat tgtatgatgg taggcaatga tgtcgaggag
     1021 gatctgatcg cgggccagtt gggaattgaa acttttttaa tcaccaatta cctgattcaa
     1081 aaaccaggta ccaacatcgt ctgtgattgc caaggggatt atgatgcgtt ttaccggttc
     1141 gtcacgtcgc tccctgagat tggatttaaa ggggcgtgat ccggatgagt actgtgcgat
     1201 tgcgaagctg attgagggaa gaatcattcg ggtacagtaa cgaagcctct cccagactgc
     1261 ttacagtgaa cattgacgcc ggaagatcgg cttgggcacg taaatagtca accacgatga
     1321 ctgcccgttg catacctaaa gatacgttat ccctgtagat aagtccgagc ggcatgggta
     1381 cattgtccgt gtgaccctcc acatgcacca gaacccggtt tttgtagggg cgaagctgga
     1441 gggcaaggtt ttttaggctg gcttgggccc cgggcaataa cttcactccg cgctgaaaca
     1501 gccccgtgtt aaagaccacc gtaatctgac ctttccggta attcacattt gctccgggga
     1561 tccgcaaatc tttggcgaat gcctgaggca gatcccgggt ctgtactcga tcccggggcg
     1621 tttcctccgg aatcctccga ggggccgttg atatgcgttg gaataccggt acgggaagtc
     1681 gtactgtcct cagctctccc aagtaatgcc gggctgagaa acccatcata acaaagcaaa
     1741 gtaaaattcc gatcccccag agccagcctc gaagcggtcg ttccgcagcc attccgtttt
     1801 gcaatctgat ccggcgtagc cccaactggt attcccgatt ggtaggatca agccgcagcg
     1861 ctgtactcca agagatctcc gcctccgtgt aacgcccttg ttgggcctga atccgcgcca
     1921 gcaaatcata aacctccggt tgttcaccgc tttcctggac aagcagtaac aagaggttct
     1981 cggcatcagg gtaatgaccg gagcgggcca atttaacggc ttgcggcaaa acgatcttgt
     2041 caagcagatt tgcggcggag cctgagtctt cccggtccac cgactcattc atgctctctg
     2101 ccggtaatga atatacagta tcccgcctgc ggcccatccc caaccctgcc tttctgcctt
     2161 cgttcctcat tcctcttccc ccggcaggcc aacaaccact tcgcccatct gaatcgggcg
     2221 ttcctgtaga aaaatagttg gtctcgcggt ttgaatcacc cgggcttggg taaaaccggc
     2281 gactttttcg aaagacaata cccgaagcgg catattggcg gtatagatgg tatcggtgtg
     2341 gtccaaaatc atgaccccga ctcttgccag atcgttccat atctccttca tcgaacgaca
     2401 cgcgcggcgc atccccggtg tggggtgatc ggtaccgggt tccatcatct tacgttgtaa
     2461 acgccagagc ccggttccta cctctgccag gtattgcatg gattcggcac ctgcgctttg
     2521 aactgataca tccccggcaa gccaagcgat ttttcgcagc accatttcca ggtcgattgt
     2581 ttcggacggg gggattcgaa attcccacgg gtaacgcagc tgtcgtaaac cggccatcag
     2641 cacaaccata atcctcccgt agttttcttg ggcttacatc tctttgattt ccgccttctt
     2701 taatcattcg accccggatc tggttttcct gccagatata cccgggatgg atttagataa
     2761 aatcagaccg gtaaccggat tagccccggc ttacgcgtcc tccgggtaaa gcccccaggt
     2821 tcctgccggc ggcttcttga gttccgccaa cccgtaacgt gttgttcgct tcatcccatt
     2881 tccagtaaaa ccccgaatta ttgccttcat agaattgcag attgaaatag tcatgaaaag
     2941 tcggatcgac cccattcgct gtctccaccc gccaggttgt aaataccgat ttggcgagat
     3001 agatcgaacc gttccagtaa aacccgtccg gatttgaacc gtttccaggt tcaatggtct
     3061 cggcaggacc gggctccgtt tcgatgatcc gaatcacccg cggcaacgga aattttaatg
     3121 gacgtttggg atccttgatg agttccgcca atgctctcag gtgcacctga caattccagg
     3181 ccagattgtc gatggcgtta agtacttttg catcctgatc cccccagtgg taaatatcag
     3241 ggaacaattc cagccaggcc tggttttgct ggtcgatact cgggcggacc gtttcgtaaa
     3301 cgatatccac cggcgttccc acggaaacca gcccaaaaac ctcggcgata tcctggttcc
     3361 gcatccggaa acatcccagc gaaaccgaac ttccaatcga gcttggct
//
LOCUS       DASVMB010000216         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_689855,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000216 DASVMB010000000
VERSION     DASVMB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689855"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF531_06455"
     CDS             <1..320
                     /locus_tag="VF531_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713645.1"
                     /translation="EDELKTGTRREGMYFGINGFMVRIGISLQSVIMGSVLSLSGYNA
                     KLPVEAQSAGTFLGIKFLLVVIPLVSLVFAILFYRLYPLHGAKLRAVKEKLLSIHDKS
                     IRG"
     gene            555..1559
                     /locus_tag="VF531_06460"
     CDS             555..1559
                     /locus_tag="VF531_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7713646.1"
                     /translation="MNPTIKDVAVTANVSVATVSRVLNDLPGYSEETRQKVLQAITKL
                     GYEPNALARGLVSKRSHTIGILMPSLSSMVASEILKGIEDTAHRSNQSVIVCNTDNDG
                     KRTLEYLQVLQEKQVDGIIVVSANIIPEYYQKMMAMRKPVILVATKYPQSNLPFIKVD
                     DEKAAYDATSYLIQKGHRIIGMLSGTRTDQIASTPRVKGYERALSDHGIPIRENYITY
                     GDFSFKSGMICLERLLEATPEITAIFAASDEMAVGVLSQAFKMGIAVPRQLSVIGYDN
                     TQIAEMTTPPLTALSQPFYEMGQSGIAMLLEQIEQGKKMESLIMPHRIAERDTVREIL
                     "
     gene            1676..2893
                     /locus_tag="VF531_06465"
     CDS             1676..2893
                     /locus_tag="VF531_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713647.1"
                     /translation="MRKKVGLMLLAFLLVLASGTMVPVNAAKVKLELFQNKREAVKTF
                     DELIAKFMKEHPDIEVVQNFTPEAETVIKARLVKNDIPDIMAIGGNFTYGELARAGVL
                     ADFSKDSGLKNVQPAYVKMLNALAQLKTVNGIPFSANANTVLYNRDKFAKLGLTVPKT
                     WDEFVAMSEKIKKTGEVPFYLTFKDSWTVMVPFNTLASNLHGDDFFDKRRANKTTFKA
                     RYPEVATKMLTLLKYGHNDNFGMGYDQGNTAFANGQSVMLIQGVWAIPPIKAANPNIK
                     VGAFSLPATNSAAKNRLVSGVDTVLTMSKTTKNQKAALEFIHFLLKKENAQYYIDEQK
                     LFSAVKGVYQEDPELVDLKAYFESGRITSFADHYYPSGMQVSNLVQEFLQKGNVNQFL
                     TNMDQEWDKTISR"
     gene            3029..>3239
                     /locus_tag="VF531_06470"
     CDS             3029..>3239
                     /locus_tag="VF531_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713648.1"
                     /translation="MPLVYFLFTIPALVLFFAFHTFPALQGIYYSFTNWPGYGNSDWV
                     GLKNYFNLFKDQRVWESYLFTFKFAI"
BASE COUNT          880 a          703 c          797 g          859 t
ORIGIN      
        1 atgaggatga actgaaaacc ggaacccggc gtgagggaat gtatttcgga atcaacggtt
       61 tcatggtccg aatcgggata tcgttgcaat cggtgattat gggttcagtc ctgtcactct
      121 ccggatataa tgccaagctt ccggtagagg cccaatcagc aggaactttt ttgggaatca
      181 agttcttgct ggtggttatc cctctggtgt ctttagtttt tgcgattcta ttttaccggc
      241 tgtatccgct ccacggggcg aaactccgcg cggtcaaaga gaagcttcta tcgatacatg
      301 ataaaagcat caggggataa gttctattcg tcacaaaaga cgttctacct tacttaggga
      361 ataagtaagg cagaacgtct tttttaatgt acaaccctaa tattcgccgt accggaacgc
      421 tctatcaaca taggctattg tctatttcta aaactattgg tataatgaaa ttattaataa
      481 aatattattt gtttttatat tttgcgtaaa cgtttacgtg atacgtaaac gtttacgttg
      541 ccgggggttt tgtgatgaat ccaaccatta aagatgtggc tgtaacagcc aatgtatccg
      601 tggctacagt ctcccgggtt ttgaacgatc tacccggtta ttcggaggag acaagacaaa
      661 aagtcttgca agccattacc aaactgggat acgaacccaa cgccctggcg cggggattgg
      721 tcagtaaaag gtcacatact atcggaattt taatgcctag cctgtcaagc atggttgcct
      781 ccgaaatttt gaagggcatc gaagataccg ctcatcggtc caaccagagc gtcatcgtat
      841 gcaacaccga taatgatggg aaacggaccc tcgagtactt gcaagtctta caggaaaaac
      901 aggttgacgg gatcatcgtg gtcagcgcca atattattcc ggaatattat caaaagatga
      961 tggccatgag gaaaccggta atattggttg cgaccaagta cccccaaagt aatttgcctt
     1021 ttatcaaagt ggatgatgaa aaggcggcct atgacgccac ttcatacctg attcaaaagg
     1081 gccatcgtat catcggaatg ctcagcggaa cccggaccga tcaaattgcc tctacacccc
     1141 gggtaaaagg gtatgagagg gcattgagcg atcatggcat tccgattcgc gaaaactata
     1201 taacctacgg ggatttcagt ttcaaatccg ggatgatttg cctggagcgg ttgttggaag
     1261 caaccccgga aattactgcc atctttgccg ccagtgacga gatggccgtc ggagtcctgt
     1321 ctcaagcttt taaaatgggg attgccgtgc cccggcaact ttcggtgatt ggttacgata
     1381 atacccagat cgcggagatg actacaccgc cgttgaccgc cttgagtcaa cccttttacg
     1441 aaatgggaca gtcgggtatc gccatgttgc tggaacagat cgaacaaggc aagaagatgg
     1501 agagcctgat catgcctcat cgaattgcgg aaagggatac ggttcgggaa attttatagg
     1561 gcgttcggat tttgggattc cacgcctttt cgaatatgga gcggaggtga tgacggggaa
     1621 gtattggttt gtgtttcagg gggattcata tcaaaattag gggaggggca ttttaatgag
     1681 gaaaaaggtt gggttgatgc ttttggcatt cttgttagtc ctggcttcag gaacgatggt
     1741 tcccgtaaac gcggccaaag ttaagctgga acttttccag aataagcgtg aagcggtaaa
     1801 gacctttgac gaattaatcg ccaaatttat gaaagaacac cctgatatcg aggttgtcca
     1861 gaattttacc ccggaagcgg aaacagtcat caaagcccgt ctggttaaaa atgatattcc
     1921 cgacatcatg gccatcggtg gcaattttac atatggcgag ttggccaggg ccggggtatt
     1981 ggctgatttc tctaaagatt cgggtttgaa gaatgttcaa ccggcgtatg tcaagatgtt
     2041 gaacgcgctg gcccaattga agaccgttaa tggaatccct ttctccgcca atgcgaacac
     2101 cgtgctttat aacagggata aattcgccaa actgggcctg acggttccca agacatggga
     2161 tgagtttgtc gccatgtccg agaagattaa aaagaccggg gaagttcctt tctatttgac
     2221 atttaaagat tcctggactg tcatggtgcc ttttaatacg ctggcctcca atttacacgg
     2281 cgatgacttc tttgataaac ggagagccaa caagaccaca tttaaagccc gttatccgga
     2341 agtggcgacc aaaatgttga ccctcctcaa atacggtcat aacgacaatt tcggaatggg
     2401 ttatgatcag ggcaatacgg ccttcgccaa cggccaatcg gtcatgttga ttcagggggt
     2461 ttgggctatt ccgccgatta aggccgctaa tcccaatatt aaggtgggag cgttttctct
     2521 cccggcaacc aacagcgcgg ctaagaacag gttggtttcc ggcgtggata ctgtgttgac
     2581 catgtctaaa accaccaaga accaaaaagc cgccctggag tttattcatt tcctgcttaa
     2641 aaaggagaac gcccaatatt acatcgacga acagaagctt ttttcggcgg tcaaaggcgt
     2701 ctatcaggaa gatccggagt tggtcgactt gaaggcatac ttcgaatcgg gccggatcac
     2761 cagtttcgcg gatcattatt atccttcggg gatgcaggtt tccaatctgg tacaggaatt
     2821 tctgcaaaaa ggcaatgtga accaattcct taccaacatg gatcaggaat gggataaaac
     2881 catttcccgc taacatcatt ttggtggaac agatttgcgc ccgaggcgca aatctgttcc
     2941 attaaattta aacgggagtt gaatcaaagt gcagactgag cttatattgg accggaagtc
     3001 ggcaccactt aagaaaaaga aaccgaaaat ccccttggtt tactttctgt ttaccattcc
     3061 ggctttggtg ctgtttttcg cctttcatac cttcccggcc ttgcagggaa tctattacag
     3121 cttcacaaat tggcctggtt acgggaactc ggattgggtc ggattgaaaa actatttcaa
     3181 cctctttaaa gaccagcggg tctgggagtc gtatctcttt acatttaaat ttgccatta
//
LOCUS       DASVMB010000217         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_689939,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000217 DASVMB010000000
VERSION     DASVMB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689939"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF531_06475"
     CDS             complement(<1..401)
                     /locus_tag="VF531_06475"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HEX7713649.1"
                     /translation="MHQYIDQLQKLIQKVEQTQEENIKAAAALIAATIRNGGVLHVFA
                     SGHSHMLMEEIFYRAGGLVPVNPIFEPSLMLHHGAAKSSSFERVEGIAGTILGQVETK
                     PGEPIIIISNSGINAVPVEMAKEAGALGMKV"
     gene            complement(416..1141)
                     /locus_tag="VF531_06480"
     CDS             complement(416..1141)
                     /locus_tag="VF531_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7713650.1"
                     /translation="MINKDSSIPLYIQIKDVWKEQIERNLLKPGDQIPTENELCKLYD
                     VSRITVKQAINSLVNDGYLVRSRGKGTYVKPPKLEQNLAGVTSFTEDMIRRGLKPGAT
                     LLNTEVETAEPDIAARLQLGDGARVIKVQRLRLADGQPMAIETIWLPYHLCPTLLQED
                     LTTSIYQILKNQYKIVFNRAVQYLEASKAEKREAKLLQIEPGDSVLEMERQSYMKDNV
                     PVEFTQSVYRGDKYKFRVELSGQ"
     gene            complement(1222..1962)
                     /locus_tag="VF531_06485"
     CDS             complement(1222..1962)
                     /locus_tag="VF531_06485"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HEX7713651.1"
                     /translation="MLIDKYFSNMKEVLNRIEATQTGRIKDASGMIVSSLKNGGAVHL
                     LDTGHMLSHEVIGRAGGLMLITPINIGINIDNPARIRKGQEKKQVFWDEIEGLPGYIL
                     NKSKVFAGDVLIIASVSGKNVLPVETALQARTMGVKTIAITSVEYSRNLKSAHSSGKL
                     LFEAADLVLDNCCPYGDAVVGDEKLQVKICPGSGIAGAYLMWALTAQITEDLMNSGVK
                     PSIYMSNHLEGADEANRGAMRVFQETGF"
     gene            complement(1980..2780)
                     /locus_tag="VF531_06490"
     CDS             complement(1980..2780)
                     /locus_tag="VF531_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7713652.1"
                     /translation="MSFLPRVDYHIHSVFSDGEGDLGSILRMAKSQMLECIAITDHYD
                     PYQPAGAGNPNQNQPDLKTFRNRFFKARLEIDSPDEPQMIFGAETGPVSSEALYETCD
                     MVIGSVHYVTGYTLNSEEKGIVFNEKYWDAYRDTVIKIIKDPYIDIIGHIEGYLPLAP
                     LLNRQTTFEERREMDREIARRYFTDGFWEKVIQNALRYGKVIEVHGMTQTPRPYYLQK
                     AVRAGISVSVGSDAHRLSDIGRIDWGLNILTEIGLPAGRLFLGRNRTK"
     gene            complement(2859..>3995)
                     /locus_tag="VF531_06495"
     CDS             complement(2859..>3995)
                     /locus_tag="VF531_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7713653.1"
                     /translation="KGGMLDALKEECEKANPDIEINYIKVPGQEYYTKLLTMIASGDV
                     PDMAMLGNDWLAPYASKGALEPLDRLIASKKFPMKEIYPSVQACLKYKGKTYALPRDT
                     TSNVLFYNKKLFDQAGVKYPSENWKWADFLAAAKTLTKVDQNGKTVQWGFYFDTYADG
                     WYHWLLTNGTSFVNSKFNKSNMNTPQVIETLKFLSDLMIKYKVAPDVGQAQGLTGEEG
                     VFLGQQAAMYIGGVSRAVNFAKDPNLDWDVAPIPYNKEKTSRVWTNLWVIPKGSKQPN
                     AVWKVMSFYAGPEGQKIAAKLNSGIPSMKSIAESPVFLSGPPAHRKYFLDAFENGKPF
                     PSFPECNEFLTIMIRELQPVWIGERSVEDAVKTIDTLANKLFVK"
BASE COUNT         1012 a         1051 c          815 g         1117 t
ORIGIN      
        1 ataaccttca tgccgagcgc gccggcttcc ttcgccattt ccaccggaac cgcgttgatt
       61 ccgctattgg agatgatgat gatgggttcg ccgggtttcg tctccacctg ccccaggatg
      121 gtccccgcaa tcccttcaac cctttcaaaa ctgctgcttt tggcagcgcc gtgatgcaac
      181 atcaggcttg gctcaaaaat ggggttgacg ggaacaagtc ctccagcccg gtaaaatatc
      241 tcttccatca gcatatgaga atgacccgaa gcaaaaacat gcaagactcc gccatttctt
      301 atcgtagcag caattagcgc ggcagcggcc ttgatattct cttcctgggt ttgctccact
      361 ttttgaatga gcttctgcag ttggtctata tattggtgca tcagatcttc tcctttcatt
      421 gcccggacaa ttctacccta aacttgtatt tgtcaccgcg ataaacggat tgggtgaact
      481 ccaccggaac attgtctttc atatagcttt gacgctccat ctcaagaaca ctgtcgcccg
      541 gttcgatctg aagtaatttg gcttctcttt tttccgcttt agaagcctca agatattgaa
      601 ccgcccggtt aaaaacaatc ttatattgat tctttaagat ttgataaatt gaagtcgtta
      661 aatcttcctg caaaagagtg ggacaaagat gataaggcag ccaaatcgtt tcaatggcca
      721 tcggttgacc gtcggccaac cgtaatcttt gcaccttaat cacccgggcc ccgtcaccta
      781 attgcagtct ggcagcaatg tcgggctccg ccgtttcaac ctcggtattc aatagcgtgg
      841 ctcccggctt taatccccgg cggatcatgt cctctgtaaa actggtgact ccggccagat
      901 tttgttccaa tttaggaggt ttcacataag tgcccttgcc tctgctccga acaaggtatc
      961 cgtcattgac cagcgaatta atggcctgct tgacagtgat ccggcttaca tcataaagct
     1021 tgcataattc attttccgtg ggtatctgat caccgggttt caacagattc cgctcaattt
     1081 gttccttcca aacatcttta atttgaatat ataagggaat ggaactgtct ttattaatca
     1141 ctattaaata cccctttttt taacgtatca agttcatgga gaaaaagctg ttccaacatc
     1201 aaccgatacc acctctcctc actaaaaacc ggtttcctga aacacccgca ttgccccacg
     1261 gttggcctca tccgctccct ccagatgatt gctcatatat atactgggtt tcactcccga
     1321 attcattaag tcttcggtaa tctgtgccgt caatgcccac atcaaatagg ctccggcaat
     1381 gcctgatccg gggcaaattt taacctgcag tttttcatcc ccaacaacgg catctccata
     1441 aggacaacaa ttatccagca ccagatcggc tgcttcaaac aggagcttgc cggaactgtg
     1501 agcggatttt aaattccgtg aatactctac cgaagtgatg gcgatggttt tgactcccat
     1561 cgttcgcgct tgcaacgccg tctcaacggg caggacattc ttccccgaaa ccgaggcgat
     1621 gatgagtaca tccccggcaa aaaccttgct tttattcaga atatacccgg gaagcccctc
     1681 aatctcatcc caaaacacct gctttttttc ttggcccttc cggattcggg ccggattatc
     1741 aatattgatg cctatattta tcggggtaat gagcatcaaa ccgccggcgc gtccaataac
     1801 ctcatgagaa agcatatgac cggtatcgag gagatgaacg gctccgccat ttttaagcga
     1861 actgacaatc atccccgatg cgtccttaat cctcccggtc tgcgtcgctt cgatccggtt
     1921 caatacttct ttcatgtttg aaaaatactt atcaatcagc aatgttgtaa ctcctctcat
     1981 catttggtac ggttacgtcc caggaataat cgacctgcgg gcaatccgat ttccgtcaat
     2041 atatttaaac cccagtcaat tctgccaata tcgcttaaac gatgggcatc gctgcctaca
     2101 cttactgaaa ttccggcccg aaccgctttt tgaagataat acggcctggg agtctgagtc
     2161 atcccgtgaa cttctataac tttaccatac cggagggcat tttgaattac tttttcccaa
     2221 aacccatccg taaaataacg ccgggcaatt tcccggtcca tctccctccg ctcctcaaag
     2281 gtagtctgac ggtttaaaag aggggcgagc ggaagataac cctcgatatg accaatgatg
     2341 tcaatatacg gatctttgat aatctttatt accgtatccc ggtatgcatc ccaatacttt
     2401 tcattaaaaa ctatcccttt ttcttccgaa ttcaacgtat aaccggttac ataatggacg
     2461 ctgccgatta ccatatcaca tgtctcatat aaagcctcgc tcgaaaccgg tccggtctct
     2521 gctccaaaaa tcatttgcgg ctcatcggga gaatctatct ccaggcgcgc cttgaaaaaa
     2581 cggtttcgaa aagtcttcag gtcaggttga ttttgatttg ggttcccggc tccggccggt
     2641 tgatacggat cataatggtc tgtaatcgcg atgcattcca acatttggga ttttgccatc
     2701 cggaggatgc ttccgagatc gccttctcca tcggaaaaaa ccgaatgaat atggtaatcc
     2761 acacgcggta aaaaactcat cgcaaataca ctccgcatct atctgccgct gatgggacag
     2821 gagtaaagca acctcccgtc ccaccggcag ttatcgtttt atttcacaaa gagtttattg
     2881 gctagggtgt caatggtctt gacggcatct tccaccgaac gctcgccaat ccaaaccggc
     2941 tgcaattccc ggatcatgat ggtcaaaaat tcattacatt cagggaatga cgggaacggc
     3001 tttccatttt cgaaggcatc caggaaatat ttcctatggg ccggcggacc agacaaaaag
     3061 acgggagatt cggcaatcga tttcatcgat ggaattccgc tattgagttt ggcggcaatc
     3121 ttctggcctt ccggtccggc atagaaggac ataactttcc aaactgcatt cggctgtttg
     3181 cttcccttgg gaatgaccca tagattggtc cagaccctgg aggtcttttc cttattgtaa
     3241 gggatagggg caacatccca atccaggttg gggtccttgg cgaaattaac cgccctggac
     3301 actccgccga tatacatggc agcctgttga cccagaaaaa cgccttcttc ccccgtgagt
     3361 ccctgggcct gtccgacatc gggcgccact ttatacttga tcatcagatc ggacaggaat
     3421 ttcagggttt caatcacttg gggcgtattc atatttgatt tattgaactt ggaattcaca
     3481 aaactcgtcc cgttggtgag cagccaatga taccaaccat ccgcgtaggt atcaaaataa
     3541 aatccccatt gaaccgtctt tccgttctgg tcaactttgg taagcgtttt cgcagcagct
     3601 aaaaaatcag cccatttcca attttcggaa ggatatttta cgcctgcttg atcaaatagt
     3661 tttttattgt agaacaacac attactagta gtatcacggg gcagggcata cgtttttccc
     3721 ttatatttca aacaagcctg gactgacgga tatatttcct tcatcggaaa tttcttgctg
     3781 gcaatcaaac gatccaaagg ttccagagcc ccttttgaag cataaggagc cagccaatca
     3841 ttgccaagca tcgccatatc gggtacatca ccggaagcta tcatcgtcaa aagtttggta
     3901 taatactctt gacccggaac cttgatataa ttgatttcga tatcaggatt cgctttttca
     3961 cattcttctt ttaatgcgtc cagcatgccc ccctt
//
LOCUS       DASVMB010000218         4469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_248943,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000218 DASVMB010000000
VERSION     DASVMB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248943"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF531_06500"
     CDS             complement(<1..314)
                     /locus_tag="VF531_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX7713654.1"
                     /translation="MLTNLRTKLTSSHIIVALAGILLTSLLANLLLENQFRQYIKANL
                     RRQNQRIIAQIAQNYNTTISGWNPNDLTEIGMNALSQGLILKLKGTDGAIIWDATVHN
                     NG"
     gene            complement(320..1018)
                     /locus_tag="VF531_06505"
     CDS             complement(320..1018)
                     /locus_tag="VF531_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713655.1"
                     /translation="MKVQQRTILVIDDEPQIVAVVESYLENSGYQVSSAETGRAGLDL
                     FERVQPCLVILDLMLPDIPGEEVCSRIRKKSHVPIIMLTAKVDEEEVLEGLGLGADDY
                     VTKPFSPRQLVARVEALLRRTDRNSIPLANRIAFGDDLVIDGLKHEVRKQGEIINLTP
                     HEYSILMLLAKYPSKTFTREELVEMVLDESYEGYQRTIDAHIKNLRQKIETDPHHPRF
                     IRTVHGIGYRFGGD"
     gene            complement(1210..1422)
                     /locus_tag="VF531_06510"
     CDS             complement(1210..1422)
                     /locus_tag="VF531_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HEX7713656.1"
                     /translation="MSDQVMTFDVEGMSCNHCVNSIKNAVGALDGVKQVDVDLKGKKV
                     TVGVSAGKTTAQAIREAIEDQGYEVK"
     gene            complement(1456..3876)
                     /locus_tag="VF531_06515"
     CDS             complement(1456..3876)
                     /locus_tag="VF531_06515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7713657.1"
                     /translation="MEKKEVLPITGMSCAACALKIEKNLAKVNGVKQANVNFATEKAT
                     VEYDSEQVNREQLEATVKRLGYDVVAEAQENAKVTLKITGMTCAACAARIEKQLGKMV
                     GVTKAAVNLATEKATVEYVPSQVKLSEMIKTVTSLGYGAEPESVMDEAGRDQEKERRE
                     KELRRLRWELIISAVMSSPLILAMVLTLFGINLAFLHDWRFQILLATPIQFGIGFRFY
                     RNAYFALRAKSPNMDVLIAMGTSAAYLYSVYNAFFQKLAPGMMMKDLYFEAAATIITL
                     ILLGKYFEAVAKGKTSEAIKKLMGLRAKTARVIRQGVEQDIPIEEVEVGEVIVVRPGE
                     KVPVDGRVIEGSSAVDESMLTGESLPVEKHTGDQVIGATINKNGTFKFEATKIGKDTV
                     LAQIIKMVEDAQGSKAPIQKIADRVSGIFVPVVLGIALVTFLVWTFGDYNLKMGIVSA
                     VAVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGESLEKAYKINAVVLDKTGTIT
                     KGQPEVTDIIPLGKLERSELLKLAAIAEKKSEHPLGTAVYEQGQKELGPIPDPQDFRA
                     IPGHGIAARIEGKEVLIGTRKLLTEQGIDLRDHETKADGLEQAGKTVIFQAVGGEFAG
                     IIAVADVVKETSAAAIAELRQLGIEVYMLTGDNQRTARAIAAQVGIDQVLAEVLPEHK
                     AAEVEKLKKTGKVVAMVGDGINDAPALAVADIGMAIGTGTDVAMEAADITLMRGDLRA
                     IPTAIRLSRKTMQKIKQNLFWAFFYNSIGIPFAALGMLNPVIAGGAMAFSSVSVVTNS
                     LSLKRFRG"
     gene            complement(3950..>4469)
                     /locus_tag="VF531_06520"
     CDS             complement(3950..>4469)
                     /locus_tag="VF531_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal transporter"
                     /protein_id="HEX7713658.1"
                     /translation="QISQKDVDQVNNSSSSGGITGGAGCCAASAQATRFYGGRIPTDD
                     LHIAKIKDGVQEVSITVNDQGYAPAAVVLQRGVKARLVFIPEKLNSCNYAVYFPEYQA
                     QLDMSKGQTETPLIDVTRDFTFQCWMGMLHGYVKVVDDLNNVNLTEIKKEIDAYRPAG
                     GGGGCCGGGGGY"
BASE COUNT          990 a         1311 c         1041 g         1127 t
ORIGIN      
        1 aatccgttgt tgtgaaccgt ggcatcccag ataatcgcgc cgtcggttcc tttgagctta
       61 agaatcaacc cttgagacag ggcgttcata ccaatctccg taagatcatt agggttccaa
      121 cctgaaatcg tagtattgta attctgggcg atctgggcga tgatccgttg attctgtctt
      181 ctcaggttgg ccttgatata ctgccgaaat tgattttcca acagcagatt tgccagcaag
      241 ctggtgagca ggatccctgc caaagccaca ataatatggg atgaggttag tttggtccgg
      301 aggttggtca gcatgatcct taatcacccc caaaacgata cccgatgccg tgaacggtcc
      361 ggatgaaccg gggatgatgg ggatcggtct cgattttttg gcgtaaattt ttgatatggg
      421 catcgatggt ccgttggtag ccctcgtaac tctcgtccag aaccatctcc accaattcct
      481 cccgggtgaa cgtcttgctg gggtatttag ccaaaagcat cagaatgcta tattcgtgag
      541 gggtcagatt gataatctca ccctgtttgc gtacctcatg ctttaaaccg tcaatcacca
      601 gatcatcacc gaaagcgatc cggttggcga gcgggattga attgcgatcg gttctgcgta
      661 acaacgcttc caccctggcc accaattgcc ggggactgaa gggcttggtc acgtaatcgt
      721 cggcacccaa acccaaccct tcgagcacct cttcctcatc gaccttggca gtgagcatga
      781 tgatgggaac atgggatttt ttccggatcc ggctgcaaac ttcctctccc ggtatgtccg
      841 ggagcatcaa atccaaaata accagacagg gctgaacccg ttcaaagaga tccaaccccg
      901 cccggccggt ttcagccgaa ctaacttggt aaccgctgtt ttcaaggtaa gactctacca
      961 ccgccacaat ctgcggttca tcgtcgatta ccaaaatcgt ccgttgttgt accttcatct
     1021 ttcctccata tcaagtgcgc atgattgatc tttggttcat gccggagaac ggtggatcct
     1081 tacatatcta tcattcataa acgcgcgcgg cgcgaaaagc aaaaatgctt gatcatgctg
     1141 aaaacccctt cccaaaagaa ggggttttca gctagcagtc ataaatcggc agatttacca
     1201 gacgactact tacttcacct catacccctg atcttcgatg gcctcccgga tggcctgggc
     1261 ggttgtcttt ccggcgctta cgccgactgt gactttcttg cccttgaggt ctacatccac
     1321 ctgcttgaca ccatccagag cgccgaccgc gttcttgata ctattgacac aatggttgca
     1381 ggacattccc tcgacatcaa aagtcatgac ttgatccgac atatgaatca ttcctttcta
     1441 ttgtagttta acgccttaac cccgaaaccg tttcagactt aacgaattgg taaccaccga
     1501 gaccgaactg aaggccatag ccccgccggc aatgaccgga ttgagcattc ctaatgccgc
     1561 gaaggggatg ccgatggagt tgtagaagaa agcccaaaac aggttttgtt taatcttctg
     1621 catcgtttta cgggataacc gaatcgctgt gggaattgcc cgcaaatcgc cgcgcatcag
     1681 ggtgatatcc gctgcctcca tagctacgtc cgtgccggtc ccgatggcca tcccgatatc
     1741 ggcgacagca agggccgggg catcattgat cccgtctccc accatcgcca cgactttacc
     1801 ggtctttttt agtttctcca cttccgccgc tttatgctcc ggcagcacct cggccagaac
     1861 ctgatcgatc cccacctggg cggcgatagc ccgggcagtc ctctgattat caccggtcaa
     1921 catatagacc tcgattccca gctgccgcag ttcggctatg gcagcggcgg aggtttcctt
     1981 gaccacatcg gccactgcga tgattccggc aaattcaccg ccaactgctt gaaagatcac
     2041 ggttttaccg gcctgttcaa gcccgtcggc tttcgtttca tgatcccgaa ggtcaattcc
     2101 ctgctccgtc aggagctttc tggtcccaat caaaacttcc ttgccctcga tccgggccgc
     2161 gataccatgg ccgggaattg cccgaaagtc ctggggatcg gggatgggtc ccagctcttt
     2221 ttggccttgt tcatatacgg ccgtgcccaa gggatgctcc gatttcttct cggcgatggc
     2281 cgccagtttt aaaagctcgc tccgttccaa tttacccaaa gggataatgt cggtcacttc
     2341 cggctgaccc ttggtaatgg ttccggtttt atcaaggacc acggcgttaa ttttataagc
     2401 tttctctaaa gattcgccgc ccttgatcaa aataccgttt tcggcgcctt tgccggttcc
     2461 caccatgatc gccgtaggcg tggccagccc caaggcgcag gggcaagcaa tcaccagtac
     2521 cgccacggcg ctgacgattc ccatcttcag gttataatca ccgaaggtcc ataccaggaa
     2581 ggtcaccaag gcgataccca gcaccaccgg aacaaagatc ccggataccc ggtcggcgat
     2641 cttctgaatc ggtgcctttg aaccctgggc gtcctcaacc atcttgataa tctgcgctaa
     2701 aaccgtatcc ttcccgattt tggtggcctc aaacttaaaa gtgccgtttt tattgatggt
     2761 ggctccgatg acctgatcgc cagtgtgttt ttccaccggc aaactctccc cggtcaacat
     2821 cgactcatcc acagccgagc ttccttcaat aacgcgtcca tccaccggaa ctttttcacc
     2881 ggggcggacc acgatcacct ctcccacctc aacctcctcg atgggaatat cctgctccac
     2941 cccctggcgg attacccggg cggttttggc cctaagcccc atcagctttt tgatggcttc
     3001 ggaggttttg cccttggcca cggcttcaaa gtatttgccc aataaaataa gggtaatgat
     3061 tgttgccgcc gcttcaaagt acagatcctt catcatcatg cccggagcca gtttttggaa
     3121 gaaggcgtta tagacgctgt atagataagc agccgaagta cccatggcga tcaatacgtc
     3181 catattggga ctcttggccc tgagcgcaaa ataggcgttc cggtagaacc ggaacccgat
     3241 accgaactga atcggggtgg ccaaaagaat ctggaaccgc caatcgtgca ggaaagcaag
     3301 attgatcccg aataaagtga ggaccattgc caggatgagc ggcgagctca tcacggcaga
     3361 gataatcagc tcccacctta gccgccgtaa ctccttctcc cgccgttcct tctcctggtc
     3421 ccgtccggct tcgtccatga cgctctccgg ttcagccccg taccccaggg aagtgactgt
     3481 tttaatcatt tcagatagct taacctgaga gggaacatat tccacggtgg ctttttccgt
     3541 cgccagatta accgccgcct tggtcacgcc aaccatcttg ccgagctgtt tctcgatccg
     3601 ggcagcgcag gcggcacaag tcatgccggt gatcttcaac gtcactttgg cgttttcttg
     3661 agcctccgcc accacgtcat aacctaatcg cttgacggta gcctccagtt gttcccggtt
     3721 tacctgttcg gagtcgtact cgactgttgc tttctcggta gcgaaattta catttgcctg
     3781 tttcaccccg tttaccttcg ccaggttctt ctctatcttg agcgcgcagg ctgcgcagga
     3841 catgccggta atcggcaaaa cttctttctt ctccataaga tatcacctct taaaaatcaa
     3901 tccataacta ttttatccca catgaccgta aaggtaaatc ggtccgattt taataccctc
     3961 ccccaccacc acagcagcct ccgcctcccc cggcgggccg gtacgcatca atctcctttt
     4021 ttatctccgt cagattcacg ttattcaagt catcaaccac tttcacgtag ccatgaagca
     4081 ttcccatcca acactggaag gtgaaatccc gcgtcacatc aatcagcggg gtttcggtct
     4141 ggcctttgct catatccagt tgggcctggt attcagggaa gtaaaccgca taattacagg
     4201 agttgagttt ttccgggatg aacaccagcc gggccttgac tcctctttgg agtaccaccg
     4261 cagccggagc gtatccctgg tcattgactg taatagagac ctcctgaacc ccgtccttaa
     4321 tcttggcgat gtggaggtcg tccgtcggaa tccggccgcc gtaaaacctg gtggcttggg
     4381 cacttgccgc acagcagccg gcgccacccg tgatcccgcc acttgaacta ctgttgttta
     4441 cctggtccac gtctttctgg gagatttgg
//
LOCUS       DASVMB010000219         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_542853,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000219 DASVMB010000000
VERSION     DASVMB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542853"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..903)
                     /locus_tag="VF531_06525"
     CDS             complement(202..903)
                     /locus_tag="VF531_06525"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEX7713659.1"
                     /translation="MQKNDILKRIRCGLIVSCQALEAEPLHGSEIMARMALAAKMGGA
                     VGIRANTVADINEIRKTVDLPIIGIIKQEYTDSDVYITPTLNEVEQLINSAADIVALD
                     ATARKRPGGVEIETLIQRIKESGKLVMADISTVSEGIAAAEKGVDVISTTMSGYTPYS
                     PQITEPDYDLIKALSTKLEIPVNAEGRIVTPEQLINCFKNGAYSVVIGAAITRPQLIT
                     KAFVEAIDDYHQKIV"
     gene            complement(1212..2225)
                     /locus_tag="VF531_06530"
     CDS             complement(1212..2225)
                     /locus_tag="VF531_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7713660.1"
                     /translation="MKEYVYEDGVFTFEGSVSVQRTDDWVMPWRINHELLELYPYLNE
                     KKAKQPSGVRLAFSTTSVHIQLRLIEVPPGLMLDLIADGEHRETVTPDGQSPYVIFKP
                     LPAGLKQVEIWLDPRNEFKLNRVMVDEDAVIYKTNVIQKRWVHYGSSISQSLEARTPS
                     TIWTALVARKFNLHLTNLGFHSECKCEPLMAVLIRDLPADLITLKIGINLVDGDLNRR
                     TFKPAVLGMIKIIREKHPDTPLVVCSPIYTPLYDDIKGGSGYNLKEMRTVIVECVEIL
                     SRYGDWNLYYVDGLKLFGPEAIEHMPDQLHPDADGQYLLAEHFGEEVVGKILRQQETG
                     NRQ"
     gene            complement(2241..3194)
                     /locus_tag="VF531_06535"
     CDS             complement(2241..3194)
                     /locus_tag="VF531_06535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713661.1"
                     /translation="MSPIEIRELSRDFQTGIVNFWNRNLGKPYWVNEKTVSEKIFNDP
                     DLAPEASFLMTDGAKPAGFIVSKVNNSGLPECDNHAWISAFIIDKEYRNRGFGKTLYA
                     KAEAKLRESGVKKIILGGERNNFFSGIPEPTPGSEEFFRKKGYVINNDLHYDLMADLS
                     KIDFKVLPVPLNHDAAYSTTEMVPADRPGLEAFFDQSFPGRWKYEILGYLDRGGDFKN
                     VLLLRHQQTVAGFCKVFISEGPSDQDEFFGSFWGALGPVGISAAIRGKGLGKRILHDS
                     LCFLQGCGAHNVLIDWTVLKNFYGQFGFEPRRTYRGAYKCF"
     gene            complement(3224..4594)
                     /locus_tag="VF531_06540"
     CDS             complement(3224..4594)
                     /locus_tag="VF531_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7713662.1"
                     /translation="MIEYEVQQRYPVSEETYDVIVVGGGTAGATAAIAAAMIGANTLV
                     VERNGFLGGSSSGGQVTPMMHAGLVGNADSSSINADLKRRLCKVGYGAADPYENDGWF
                     NPEMLKFIVEDMYLEKGGHVLYDTEFIEAVCEDGGITGIIVHNKGGMQVIQGRVVVDC
                     TGDADVAFSAGAPCLTGYEANHQNQAPSLRFMVGNIDTRKLQDYLRSISQPIILEDPF
                     LELASAWETESPLRDVFQKAVSDGVLDYEDGRYFQAFSVPGMAGVLSFNCPEIQQIHD
                     CLNPYAITEIMITGRKMIQRLHRFLKLYLPGFTDSYILSAATMPGIRESRRIKGHYVL
                     SEIDYNNRAKFEDAIARTAYPVDIHGVCDEKKLAVEPMKRGEYFEIPYRSLVPLAINN
                     LLVAGRCISTTFIAQSAIRIQAVCRATGEAAGIAAAYCARSQINCNEFDGRIVRRIMI
                     QNGACL"
     gene            complement(4626..>5715)
                     /locus_tag="VF531_06545"
     CDS             complement(4626..>5715)
                     /locus_tag="VF531_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HEX7713663.1"
                     /translation="DITGFGIYQSDLVPHYSKYSTDFDNSDYLQKYIRPAHERGLKIY
                     AAVDVFAEGLIRHPHPLAPGYKNPEWATRMYGIDPAGAPVIKPITDLSGIRTTGSIDD
                     FGDVFVNPVREDVRDYELGIIRELANQYEIDGVVLDRVRFVGLGSDFSDYSRTEFEKF
                     IGRNVNNWPEEVYRLVDRNGAVEVEYGPLFGDWTTFRAQIIKEFIIAAREITREAGRK
                     VEFIDYTGSWYPLYYQVGANWAGSCYQPEEYPWVDKEYAATGYAEYLDKLLSGFYYPD
                     VTIGDALRHGKPAYWYSVEGSGELAKKVVGEAAPIIGSLYAQQYVGNPEAFSRAIDMC
                     FKKSRGCMLFDLSQIDDYQWWRVCSRAF"
BASE COUNT         1393 a         1602 c         1231 g         1489 t
ORIGIN      
        1 ccgggcgccc ccatgaacat atcccaggct tttaagggaa ctatgtacaa ttaacgtatc
       61 cccctctatc aaaccaaggc tgcgaaaacc ttccgtcaga ttgcgcggta ttacgtcggg
      121 catgtttgat gcggcccccc tcggtcaaag cgattgaaaa aagactcatt cggaaagagc
      181 gcttgataat atcgaaccgg cctacactat tttttgatga taatcatcga tagcctcgac
      241 gaaagccttg gtgattaatt gaggcctggt gatcgccgcg ccgattacca cgctatacgc
      301 accattctta aaacaattga ttaactgttc cggggtaacg atccttccct ccgcattcac
      361 gggaatctcg agctttgtac taagtgcttt gataagatca taatcaggct ccgttatctg
      421 ggggctatac ggagtatacc ccgacatggt ggtggagatc acatccacac ccttttctgc
      481 ggcggctatt ccctcggaga cggttgaaat gtcggccatt acgagtttcc cggactcttt
      541 gatcctttgg attaaagtct caatttccac accgcccggt ctttttctgg ctgtggcatc
      601 cagggcgacg atatctgcgg ctgagtttat caattgttct acctcattga gcgtcggagt
      661 gatatagaca tccgaatccg tatactcctg tttgatgatc ccgataatcg gcagatcgac
      721 agtttttcga atctcgttga tatccgcaac cgtattggca cgaataccca cagccccgcc
      781 cattttggcc gccaacgcca ttctggccat gatttcggaa ccatgaaggg gttcggcttc
      841 caatgcctgg catgaaacga tcaaaccgca cctgatccgc ttcaagatgt catttttttg
      901 catcataatc cctcttgttt ggttgattat actatctttg ccaatagtca aaagtcaatc
      961 aaagcatgag tagttgtcag ttgtttactc tattctaaag taaagtctat tggcaactca
     1021 cccccatccc ctctctttct aaaaagagag gggcgtaggg ccatactgaa agcatagtgt
     1081 atcgcggggt gagttccctc acgcctcacg cctcttgcct attccctgtt gcctgttgcc
     1141 tactatctat tgcctcgcgc cccctggggc gtgctactca tgcctcatgc ctcatgcctg
     1201 ttgcctgtcc cctattgcct gttccctgtt tcctgttgcc ttaggatttt tccaaccacc
     1261 tcttcaccaa aatgttccgc cagcaaatac tggccgtcgg catcggggtg caactgatcc
     1321 ggcatatgtt cgatcgcttc gggtccaaaa agcttcagcc catcaacata atagagattc
     1381 cagtcgccgt accgactgag tatttcaacg cattcgacaa taaccgtgcg catctctttc
     1441 agattatacc cgcttccgcc cttgatgtcg tcatagaggg gtgtgtagat cggggaacag
     1501 accaccaggg gcgtgtcggg atgcttttcc cggatgattt tgatcatgcc cagtaccgcc
     1561 ggtttaaaag tcctgcggtt cagatcgccg tcaactaaat tgataccgat ttttaaagtg
     1621 atcaaatccg ccgggagatc acgaatcaac accgccatta gcggttcaca tttacactcc
     1681 gaatgaaatc caaggttggt gagatgcaga ttaaatttcc gcgccaccaa agccgtccag
     1741 atagttgagg gcgttctcgc ctccagcgac tgactgatgg agctgccata atgaacccat
     1801 ctcttttgaa tgacgttggt tttgtatatt acagcgtctt catcaaccat gacccggttt
     1861 aatttgaatt cgttccttgg gtctaaccaa atctcgactt gttttaatcc tgccggtaat
     1921 ggtttaaata tgacatatgg cgattgaccg tccggcgtta cggtctcccg gtgctctcca
     1981 tccgcgatca ggtccagcat caaccccggg ggtacttcta tcagtcgtaa ctggatgtgg
     2041 accgaggtag tggaaaacgc gagtcgaacc ccggaaggtt gtttggcttt cttttcattc
     2101 agataaggat acaactcaag taactcatgg tttatccgcc agggcatcac ccagtcatcc
     2161 gttcgttgca cggagacact gccttcaaag gtgaatacgc cgtcttcata cacatattct
     2221 ttcattttgg ccaactccgt ctagaaacat ttatatgccc cgcgatatgt ccttcgcggt
     2281 tcaaaaccga actgcccgta aaaattcttt aaaacagtcc agtcgatcaa tacattatgg
     2341 gctccgcagc cttgcaaaaa gcataacgaa tcgtgcaata tccgcttccc caggcctttt
     2401 ccccgaattg ccgcgctgat tccgacgggg ccgagagccc cccagaaact gccgaaaaac
     2461 tcgtcctgat cactcggacc ctcgctgata aataccttac aaaagccggc caccgtttgt
     2521 tgatgccgta ataacaatac attcttgaaa tcgccgccgc gatccagata acccaagatc
     2581 tcatatttcc atcgtccagg aaagctttgg tcaaaaaacg cctctaggcc gggcctgtct
     2641 gcaggaacca tctccgtggt cgaataggca gcatcatgat tgagcggaac cggcaacacc
     2701 ttgaaatcga tcttcgatag gtcggccatc aagtcgtaat gcagatcgtt attgattacg
     2761 tatccttttt ttctgaaaaa ttcctccgat cccggggtcg gttccggaat tcccgaaaag
     2821 aagttgtttc tttccccgcc caggatgatt tttttaaccc cgctctccct cagtttcgct
     2881 tcggccttcg cgtataaggt tttcccaaac ccccgattcc ggtactcctt atcgataatg
     2941 aaagcgctta tccaggcatg attgtcacac tccggaagtc cgctgttatt gaccttgctc
     3001 acaatgaaac cggccggttt cgctccgtcc gtcattaaaa atgaagcttc cggcgccaga
     3061 tccgggtcat tgaagatctt ttccgatacc gtcttttcat tgacccaata tggttttccc
     3121 aaattccggt tccagaaatt tacgatcccg gtttgaaaat cccttgacag ttctcgaatt
     3181 tcgattggac tcaccccgcc ctgaacttaa ttgagagaaa gttttacaaa caagcaccgt
     3241 tttgaatcat gatccgccgg acaattctac cgtcaaactc attacagtta atctggctcc
     3301 gggcacaata ggctgccgca attccggcag cttcaccggt tgccctgcat acagcctgga
     3361 ttcttatggc tgattgggca ataaatgtgg ttgagataca tcttccggcc accagaagat
     3421 tgttgatcgc taagggaact agcgagcgat aggggatctc aaagtattcg ccccgtttca
     3481 tcggctcaac cgccagtttt ttttcgtcac aaaccccgtg aatatcgaca gggtatgccg
     3541 ttcgcgcaat ggcatcctca aatttggccc ggttattgta atcaatttca ctcaatacat
     3601 aatgaccttt aatccttcgt gattcccgga ttccgggcat cgtcgcagcc gaaagaatat
     3661 agctgtccgt aaatcccggc agatacagct ttaaaaaccg gtgtaaccgt tgaatcatct
     3721 ttctgcctgt aatcataatt tcagttatcg cgtagggatt taggcaatca tggatctgct
     3781 gaatctccgg gcagttaaaa gagagcaccc ccgccattcc cggcacagaa aatgcttgaa
     3841 aataccggcc gtcctcataa tccagcaccc cgtcggaaac agccttttga aaaacatctc
     3901 gcagtggact ttccgtttcc caggccgacg ccaactccaa aaacggatct tccaaaataa
     3961 tgggctggct gatacttcgt aaataatcct ggagctttcg ggtatcgata tttcctacca
     4021 taaaacgtaa ggaaggcgct tgattttgat gattggcctc atatccggtt aagcaaggcg
     4081 cgcctgccga aaacgccaca tcggcgtcac cggtgcaatc caccacaaca cgtccttgaa
     4141 ttacctgcat tccgccttta ttatgaacga ttattcccgt tatacctcca tcttcgcaaa
     4201 cggcttcgat aaactcggtg tcgtaaagga catgcccgcc tttttccaga tacatatcct
     4261 cgacgatgaa cttgagcatc tccggattga accaaccgtc gttttcataa ggatctgccg
     4321 ctccgtaacc cactttacac aaacgccgct ttaagtcggc attgatcgaa gaactgtcgg
     4381 cattccctac caagccggca tgcatcattg gagtcacctg cccgccggag gaagaaccgc
     4441 cgagaaagcc gtttctctcg accaccagcg tgttcgcacc gatcattgcc gcggcaatcg
     4501 cagccgtcgc cccggctgtt ccgcctccca ccacgattac gtcgtaggtc tcctccgaca
     4561 ccggatatct ttgttgaacc tcatattcaa tcatgttttt agcgcatccc cagcccgatt
     4621 tggaattaga aagcacggga acaaacccgc caccattggt aatcatcaat ctggctcaaa
     4681 tcgaataaca tacaaccccg cgatttctta aaacacatat caatcgcccg gctgaatgcc
     4741 tccggattgc ctacatactg ctgagcatag aggctaccga taatcggcgc cgcttcaccc
     4801 acaacttttt ttgccagctc gcccgaccct tcgacactgt accaataggc cggttttcca
     4861 tggcgaagcg catcaccgat ggtaacatcg ggataataaa agccggaaag caacttatcg
     4921 agatactccg catatcccgt agccgcatat tctttatcga cccaggggta ttcttccggc
     4981 tgataacacg aaccggccca gttcgccccg acctgatagt ataggggata ccaggaaccg
     5041 gtataatcga tgaattccac tttccgcccc gcttctctgg tgatttcgcg ggcagcaatg
     5101 ataaattcct tgatgatttg agccctaaag gttgtccaat ccccaaagag cgggccgtac
     5161 tccacttcaa cggccccgtt ccggtcgacc agacggtaga cctcttccgg ccaattgttc
     5221 acatttctgc cgataaactt ctcaaactcc gttcgtgagt aatcgctgaa atcggaacca
     5281 agacccacaa atctaacccg gtccaacacc acaccgtcga tctcgtactg gttggccaac
     5341 tccctgataa ttccaagttc gtaatcacgg acatcctcgc ggaccggatt gacgaagaca
     5401 tccccgaagt catcgatgga gccggtggtc cgaatcccgg aaaggtcggt tatcggtttg
     5461 ataaccggag ccccggcggg atcgatcccg tacatccggg tggcccactc cggattctta
     5521 taccccggag ccagtggatg agggtgccgg atcaatcctt cggcgaatac atccacggca
     5581 gcgtatattt ttaggccccg ttcgtgggcc ggccggatat atttttgaag atagtcgcta
     5641 ttgtcaaaat cggtactata cttggagtaa tgcggaacaa gatcgctttg ataaataccg
     5701 aaaccggtaa tatcc
//
LOCUS       DASVMB010000220         3135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_320612,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000220 DASVMB010000000
VERSION     DASVMB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320612"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..1430
                     /locus_tag="VF531_06550"
     CDS             477..1430
                     /locus_tag="VF531_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7713664.1"
                     /translation="MRAEDTYQTIEIGNLRLAYKLIGPGNSPILVIIETAIGSCMAEW
                     WHVAERIGVKYQVLVYDRAGYGESSVSKLERTPLNIAFELEELLRKLALDTKERIFIG
                     HSQGGLYVQQHVRRNPERSMAMILLDPLTTEDEQFRRRLTPEEYRNSGVEKTQVYKVG
                     RTLCRLKLGFIFKPLLRKSPPFYYQHFTPEAEGYILRSLTRAKQYQTAILEYRLSHQE
                     ANIAHLRENHSFPEIPIVLITHSSTISVDESMRYTGSSRETAEKVEDIWQEIMKTYLS
                     FSKSARWINASKSGHYIHLTEPELIEESLEAITKQHDAAID"
     gene            1702..>3135
                     /locus_tag="VF531_06555"
     CDS             1702..>3135
                     /locus_tag="VF531_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7713665.1"
                     /translation="MAFKWIWNELKGYRLRMALGMTLVLICSALAMINPYISGALVDR
                     VIIGKEYRLLMVFLAVMIGATLLRSALRYTFQMIFENVSQSLIFNIRVKIYTKLQNLD
                     FDFFDKTRTGDIMNKLTGDTDMTRHFVAWVIYNVFENLMLFIFAIIMLFTINYQLTLI
                     LIPVALVSGFFNVKLAKEVKPTFFAVREQLSRLNSVVQENISGNRVVKAFAKERYEIE
                     KFSKENNAFKERNMDSAGVWEKYLPVLDSMAGVLVVVIILAGGIMVIKGSLTLGELVA
                     FNSFVWAVNNPMRMFGWLINDIQRFSASGEKLIELLHTDTRIKNAPVPVVRLRLEGKI
                     EFRKVGFSYGAEEVLEEIDLQVDPGQTVAIIGPTGSGKSTLVNLIGRFYDCTSGELLI
                     DGVDIRELDLKNLRENIALAMQDIFLFSDTIEGNIAYGVPHASFKDIEWAADAAKVSE
                     FIHNFPEGYDTIIVERGVGLSGGQKQRI"
BASE COUNT          881 a          653 c          750 g          851 t
ORIGIN      
        1 gtgtttgaag aggcggcgcg cgccagaaac ctggaatctt tcaccattca tcatcccggc
       61 tcgggactgg ttacggagat tttggcagta aaaaacaacc cggaagaggg ataaaaaaat
      121 gagctatgtt cctaagaata gttgattaat atatatgttt attaaattta gtctcaaaga
      181 atatatcccg cagaggcgcg gaggcgcaga gaaagagctt acggtttttg gttatgcttt
      241 ttttgcctta acacgtttca cgtttatgag ctgatagcca atagccaaca aatgacttgc
      301 aaagctaatc ccacactccg cgcctttgcg cctctgcggg agaataataa aatggacata
      361 ttcgatcaac cattatcgga aatagagatg aaatttattt gtataatatc caatgctgga
      421 tattcattgt tggactaaat atgtgggaac aatataaaat actggaggag aagcgaatga
      481 gggcagaaga tacatatcaa accatagaga tcggcaattt aaggctagct tacaagctca
      541 ttggtccggg gaatagtccc attctggtca ttatagaaac cgccatcgga agttgcatgg
      601 ccgaatggtg gcatgttgcc gagagaatcg gcgtcaaata tcaagtactg gtctatgatc
      661 gggccggata tggggaaagt tcggtttcaa aactggaaag aacgcccctt aatatagcgt
      721 tcgaactgga agaattgctg agaaagttgg cgctcgatac aaaagaaaga atcttcatcg
      781 gccattccca aggggggttg tatgtacaac agcatgtgcg tagaaacccg gaacgttcaa
      841 tggcaatgat cctgctcgat cctttgacga cagaggatga acaattccgg aggcggctaa
      901 ctcctgagga atatagaaat agcggggtgg agaagactca agtctataag gtggggcgca
      961 ctttatgccg gttgaaactg ggttttatct ttaagccgct gcttcgtaaa tcgcctccgt
     1021 tttattatca acatttcact cctgaagcgg aaggctatat tttgagatca ttaacccggg
     1081 ccaaacaata tcagaccgcc atcctcgaat accggttatc acatcaagag gccaacatcg
     1141 cacatctccg ggagaatcat tcatttcccg agataccaat cgttttaatc actcattctt
     1201 ccacgatttc ggttgatgaa tccatgcgct ataccgggtc ctccagggaa accgcagaaa
     1261 aagtggagga catttggcag gagatcatga aaacgtatct ttcgttctca aagagcgcca
     1321 gatggatcaa tgcttcaaaa tccggtcact atattcatct gacggagccg gagttaattg
     1381 aagaatcact ggaggcaata accaaacagc acgatgcggc aatagactaa gttacagctt
     1441 agcgaaaatc atggcatatt tttataaaac tatccaaggt tgaataacca tcattggata
     1501 ataatttaca cgcaactcgc taccttatca gaatagcgaa aataaatccg ggttttcggc
     1561 tcacctaaag gatgatgaag caaaagtgaa ggtcaagcga tcttcacttt tcttatttaa
     1621 catcctgtta actatcgctt tcattttaaa tcgaattttt catagtataa tcaaattatt
     1681 aatattctgg gagggacatt cgtggccttc aaatggatct ggaatgaatt gaaaggatac
     1741 cgtctgcgta tggcactggg aatgaccttg gtgctgattt gttcggcgtt agccatgatc
     1801 aatccgtaca tatcgggagc cctcgtagat cgggtcatta tcggcaagga gtaccggctt
     1861 ttgatggttt ttttggctgt aatgattggc gccactttac tccggtccgc gctgcgttat
     1921 acgtttcaga tgatctttga aaacgtctcg caatcattga tttttaatat cagagtcaag
     1981 atctatacca agctccagaa tttggacttt gattttttcg ataaaacccg gaccggcgat
     2041 atcatgaaca agttaaccgg ggataccgat atgaccagac atttcgtggc ctgggtgatc
     2101 tacaatgtct tcgaaaactt gatgttattc atcttcgcta taattatgct cttcaccatc
     2161 aattatcaac tcaccttgat attgatccca gtagcacttg tatcgggttt tttcaacgtt
     2221 aaactcgcca aggaagtcaa accgaccttt tttgccgtcc gtgaacagct ttcccggctt
     2281 aattcggtag ttcaggaaaa catcagcggc aaccgggtgg tcaaggcctt tgccaaggaa
     2341 cgatacgaga ttgagaaatt ttccaaggaa aacaacgcct ttaaagagcg aaatatggat
     2401 tcggccgggg tttgggagaa atacttgccg gtgcttgatt ctatggccgg tgtactggtc
     2461 gtggtgatca tcctggccgg tggcataatg gtcattaaag gttccttgac tctcggcgaa
     2521 ctggttgcct tcaacagttt tgtctgggcg gtcaataatc cgatgcgcat gttcggctgg
     2581 ctcattaacg acattcagcg attttcagcc tccggtgaaa agctgattga actattgcat
     2641 accgataccc ggattaagaa tgcacctgtt cccgtcgtta gacttcggct ggaaggaaag
     2701 attgagttcc gaaaggttgg tttcagttac ggtgcggaag aggttttgga agagattgac
     2761 ctgcaggtag atcccggaca gaccgtggcg attatcggac ccaccggttc ggggaagtcg
     2821 actctggtca atttgatcgg ccggttttac gattgtacct cgggagaatt acttatcgac
     2881 ggggtggata ttcgggaact ggacctaaag aatttgaggg agaatatcgc cctcgccatg
     2941 caggatattt tcctcttttc ggataccatc gaaggtaata tcgcttatgg ggttccgcat
     3001 gcatccttta aggatatcga atgggcggcg gatgcggcca aagtatccga attcattcat
     3061 aatttccctg aaggttatga tacaatcatc gttgagcgcg gggtcgggct ttcaggcggc
     3121 caaaaacagc gaatc
//
LOCUS       DASVMB010000221         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_26984,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000221 DASVMB010000000
VERSION     DASVMB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26984"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VF531_06560"
     CDS             <1..1377
                     /locus_tag="VF531_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7713666.1"
                     /translation="KNVCLAETLNLKIRRAIFKKSIIQEGITQPPSQLTTAASKIGFI
                     DLTSFSDEVGTMPLGFNKRYSFAASLIRIAYPEQVLPGKEIIIKTAARPEDFASFSAA
                     EILSGKPFPRLQGKIVLVGLTAPGLVDWHKVANAALGRIPGVYLHAYSIANLLEWGGL
                     KKVPLLLLLLLTLVFSSFISFWPQKHLLLAAFSGFILCLVVSFNLYRQGLWISPLLSG
                     AAIVGSSLTRWSLAIVNLKSEKEKLVKIFHKYFSPQVIKEILTRPNPGAENTEKEVTV
                     IFADLRGFTGWAENSAPKKVADNLNGYLEIMSRAIINHGGIIDKYLGDCVMAFFDTGN
                     HSAAALKAALEIQLNLRNETNLPAGIGIATGVAAVGNIGPEFKKDYTVIGDVVNITAR
                     LEQLAKPGEICLTEAVARSAGITDEVFPVAVDGRIGMVNILCIKGKMIEKHQKAIRKD
                     DEKCQAEK"
     gene            1449..2039
                     /locus_tag="VF531_06565"
     CDS             1449..2039
                     /locus_tag="VF531_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX7713667.1"
                     /translation="MAVIIATKGPAWVERSGWKYSAEKGMELTAGDKIEVGQQGEVKV
                     KFFNRHTVRIGQGSELIVSKLEKEERSELVSVYLNVGQVMVEVWKRLGSIIEFSVETP
                     SAIAGVKGTKFRVAVDDLGKTQVDVTEGRVELIGKADGSKITLTRGQSSVVNPGGKPE
                     LMQKKPDALRNAPENSKQGWGMDNQSGGTGGKGGRK"
     gene            2039..2695
                     /locus_tag="VF531_06570"
     CDS             2039..2695
                     /locus_tag="VF531_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7713668.1"
                     /translation="MNAGNSINGNRFTDSSLFKEFTPGEKDEMLKKSQKVPFKKGGII
                     FEQGEESNVMYFIDSGQVRLTRFDPDGKELVLAILDPGEFFGEMALLDGFGKSCRASA
                     VTDLILSSISKQDFTTLLLEQPHLSLKVIAVLCRRLRATDQKLEEIAFGRVKDRLRSF
                     LLDSASVGKSVQLDLTHQELAAKIGACRETVTRALKELQEEGWRFEKKIIPDTSRNKS
                     "
     gene            complement(2852..3217)
                     /locus_tag="VF531_06575"
     CDS             complement(2852..3217)
                     /locus_tag="VF531_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HEX7713669.1"
                     /translation="MKICVPTIEADGMNGKVSEHFGSAPYFTLVDLESGGVEVIENQN
                     QHHSHGQCHPLASLAGKEIEAVVCGGMGRGAIQNLNSNGIKAYLGMGETVNEVIQNYQ
                     SGKLKEITMETACGGHGCH"
     gene            complement(3327..3641)
                     /locus_tag="VF531_06580"
     CDS             complement(3327..3641)
                     /locus_tag="VF531_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HEX7713670.1"
                     /translation="MGGIILPRCKKERCCRHLDNEVIYKPAGVPCPELHEVRLELDEF
                     EALRLCDLDGLDQEAAGARMGISRGTVQRLLASGRRKLIGAIVDQAALRIADPRVHTE
                     CE"
BASE COUNT         1012 a          877 c          876 g          937 t
ORIGIN      
        1 aaaaatgttt gtttggcgga aaccttgaat cttaaaatcc gccgcgcaat ctttaaaaaa
       61 tccattatcc aagaaggaat cactcaacct ccttcccaat taacaactgc tgcctccaaa
      121 attggattca tcgatttgac ctcattctcc gatgaggtcg gaacaatgcc tttaggattt
      181 aacaagcgat attcctttgc agcctcgtta atccggatag cttatccgga acaagttcta
      241 cccggtaaag agattattat taaaaccgct gcgcgccccg aagactttgc ttcgttttcg
      301 gcagcagaga ttttatcggg aaaaccgttc ccccgattgc agggaaaaat cgtactcgta
      361 gggttaactg ctcccggtct ggttgattgg cataaggttg ccaatgcggc gctgggcaga
      421 atacccggtg tctatcttca tgcatactcc atcgccaatc tgctggaatg gggcggatta
      481 aaaaaggttc cccttttgtt attgttatta ctgacgctgg tcttctcatc atttatttca
      541 ttttggccgc agaaacattt attacttgcc gcttttagcg gcttcatcct atgcctggtt
      601 gtttcattca acttataccg gcaagggctt tggatttccc cgttactttc cggagcggcg
      661 attgtcggaa gttcccttac ccggtggtcc ctggcaatcg tcaatctcaa atccgaaaaa
      721 gaaaagctcg taaagatatt tcataagtac ttttcacctc aggtaatcaa ggaaatattg
      781 acccgtccca accccggggc tgagaatacc gaaaaagaag taacggtgat ctttgccgat
      841 ctccgagggt tcacgggatg ggccgagaat tccgccccga aaaaagtggc cgataattta
      901 aacggatacc tggagattat gtcccgggcc attatcaatc atggcgggat tatcgataaa
      961 tatctcggag attgcgtaat ggccttcttc gatacaggga atcactcggc tgcagctctc
     1021 aaggcggcgc ttgagattca gcttaattta agaaacgaaa ccaaccttcc tgcaggaatc
     1081 ggcatcgcca ccggcgtggc agcggtaggg aatattggtc cggaatttaa aaaagactac
     1141 accgttatcg gggatgtggt aaatattaca gccaggctgg aacaactggc aaaaccgggg
     1201 gagatctgcc tgactgaggc agtagcacgg agtgccggta tcaccgatga agtttttcct
     1261 gtggcagtag acggccggat cggaatggtt aatatcctgt gcatcaaagg caagatgata
     1321 gaaaagcatc aaaaggcaat ccggaaggat gatgaaaaat gtcaagccga aaaataactc
     1381 ccttaacgat tataacgttt ttcattttgt tattatcttc cgtaagtttc tcgggtagcc
     1441 caaacatctt ggcggtcatc attgctacga agggaccggc ctgggtggaa cgttccggat
     1501 ggaagtactc agctgaaaag ggaatggaat taacggccgg tgacaaaatc gaggtgggac
     1561 agcaggggga agtaaaggta aagtttttta accggcatac ggtaagaatc gggcagggat
     1621 ctgaactcat tgtctcaaaa ctggaaaaag aagagcgctc agagcttgtc tccgtttatt
     1681 tgaatgttgg ccaagttatg gttgaggttt ggaagcggtt aggcagtatt atcgaatttt
     1741 cggttgagac cccctccgcc atagccggag taaaaggcac caagtttcgg gtggccgtcg
     1801 atgatctggg aaaaacccaa gtggatgtga ccgagggacg agtcgaatta ataggaaaag
     1861 ccgacggtag taaaatcaca ttgacgagag gccagtcttc cgtggtcaat ccgggaggaa
     1921 aacccgaatt gatgcagaaa aaacccgacg cgttgcgaaa tgcgccggaa aacagtaaac
     1981 aaggatgggg tatggataat caatccgggg gtaccggagg taaaggcggt cgcaaataat
     2041 gaatgccggt aactccataa acggtaatcg ctttaccgac agctctctat ttaaggaatt
     2101 tacccccggt gaaaaggatg aaatgctcaa aaagagccaa aaagtacctt tcaaaaaagg
     2161 gggcatcatt tttgagcagg gtgaagagag taatgtcatg tattttatcg acagcggcca
     2221 agttcgatta acccggtttg atcccgacgg aaaggaatta gtattggcca tcctggaccc
     2281 aggcgaattt ttcggcgaaa tggcgcttct ggacggtttc gggaagtcct gccgggcaag
     2341 cgctgtcacg gatttgatcc tgtcatcgat ttccaagcaa gattttacta cattactcct
     2401 cgagcagccg cacctctcgt taaaggtcat agcggtccta tgccgaaggc ttcgggcaac
     2461 cgatcaaaag cttgaggaga tcgcttttgg ccgggttaag gaccggctac gaagttttct
     2521 gctcgattcg gcatccgtcg ggaaaagcgt acaactggat ttgacccatc aggaactggc
     2581 ggccaaaatc ggggcttgcc gggaaacggt tacccgggcc ttaaaggagc tgcaggaaga
     2641 gggttggcga ttcgaaaaaa aaataatccc agacacctca cgaaataaaa gctaaacaat
     2701 tttacccaat agaaagccga ccgccaaaat cataaaaaca atggcagtaa tcgattgcgt
     2761 cacccaccgg ccgttcccag tcaagaccca ttttacctcg gcaatgaacc gatcaggatc
     2821 ttcattctca tgaagcacca ctctttattt ttcagtggca gccatggcct ccgcaagccg
     2881 tttccatggt aatttctttt agttttcccg attgataatt ctgaataacc tcattcacag
     2941 tctcccccat acctagatag gccttaatac catttgaatt gaggttttga attgcgcccc
     3001 gtcccattcc gccacatact acggcttcaa tttcctttcc agccaaggat gccagcggat
     3061 gacactgacc gtgactatga tgttggtttt gattttcaat tacttcaacc ccgccggact
     3121 ctagatcgac gagagtgaaa tacggagcgc tgccgaagtg ttcggaaacc tttccgttca
     3181 tcccgtccgc ttcaattgtt ggaacacaga tcttcatcgg attttcctcc atcccaagat
     3241 ttaattcata tttatattgt gcatatgcac aatataaata tagacccctg tcttggtttt
     3301 gtcaagatac agaggcctaa tatatttcat tcacattcag tatgaacccg ggggtcggca
     3361 atccgcaggg ccgcctggtc gactatggct ccgattagct tacggcggcc gctagctaat
     3421 agccgttgca ccgttccacg tgatatcccc atcctggccc cagccgcctc ctgatcgagt
     3481 ccgtccaggt cacagaggcg cagtgcctcg aactcatcca attccaatcg aacttcatga
     3541 agttccggac aaggaacccc tgcgggctta taaattacct cattatccag atgcctgcag
     3601 cagcgctctt tcttacaacg gggcagaata attcctccca taaatcaatg tttatgcccc
     3661 aaatatatga ttttttttat taaatgtcaa tccgcttaaa gc
//
LOCUS       DASVMB010000222         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_172725,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000222 DASVMB010000000
VERSION     DASVMB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172725"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..995)
                     /locus_tag="VF531_06585"
     CDS             complement(147..995)
                     /locus_tag="VF531_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713671.1"
                     /translation="MAKLKLNRKNPGLYMGIYLAAIVVVVLSVFPFLWMLSTSLKLPS
                     DTFTAVPKIIPPHFTLSNYEQAFKGTLLGRYFLNSTYISLTVTLIGIALAALGAYGFS
                     RFRFPGRKFLYLLILSVQMFPSVVIIIPLFITMRNMGLLNNHLSLLVSYTTFTLPLCI
                     WMLKGFFDGVPRELEEAAMIDGCSWGSAFFRVILPLTLPGIAASGIYAFIGAWNEYVF
                     ALTFINIDKLNTMPVGLTAFFGQFTVEWNQLMAASVLFTLPSLVFFIIVRRYITQGMI
                     TGAVKG"
     gene            complement(995..1945)
                     /locus_tag="VF531_06590"
     CDS             complement(995..1945)
                     /locus_tag="VF531_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713672.1"
                     /translation="MTPTESVSRSSRLSPLNELWGRIKKNRIAYAFIVPSLIGIGVLI
                     LYPLIQVVLISFQKYYLLEVLTKGSPYVGFENYIYLLKNPEFWYSLWITVIFTVACVG
                     LTILVGLGVALLLYQDFPGKKLVAVAILIPWVMPRVASSILWKWIYDDQFGILNYCLS
                     SLGLKSFATFPWLANASTALLAVIIVIVWQSFPFIAVSLLAGLQSIPVEVYEAAEIDG
                     AGFRQQLRFITLPLLREMIGILTVISTIWDFKVFDQMWVMTEGGPAGSTMLLGIRTWV
                     DAFALLRMGRASALAVIMLILVMLITFFYLKLFTREEEAD"
     gene            complement(2038..3309)
                     /locus_tag="VF531_06595"
     CDS             complement(2038..3309)
                     /locus_tag="VF531_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7713673.1"
                     /translation="MKRVLIILVVLSLALVSGIGMAAGETLEVWLMQTGNPEGAKMVM
                     DKMNAEFAKDHDGATVNVSWIPWTGAQQKLLTSAVAGMAPDISEVGTTWTPDFASMGA
                     IQDLDSYVAKWGQKQDLLPALVESATFNGQLFGMPWYAGNRVVYYRKDWFAEAGIKSF
                     PTTWDEFATVAKKLTKDTNRDGKIDRYGFSVNGASLHEFLPLVWMNKGEIAVKTDKGW
                     KSTIDSPEARAAIKWFADLYLKAKVSPEGSVTWNVLSSRKAFETGQQAMIIDVAPLVP
                     NYLANPELKDKFAVAPLPYSKQRASFIGGSNLVMFSQSKKKSLAWDYLQLMLSPEYQL
                     AWADTVKFFPARLSLFKSPKIANDPYLSVFTSEMKYGRTYPAVPQWGQIENSKTLLIM
                     MQQIMMRKKTVEQATTEAAKAMNIILGYSEN"
     gene            complement(3405..4268)
                     /locus_tag="VF531_06600"
     CDS             complement(3405..4268)
                     /locus_tag="VF531_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7713674.1"
                     /translation="MLKRVGEPETCIIKIRALYSTLRPSEQKVAGYILEHPQEVIYLS
                     VTELSNRVGVSDATIIKFCQRVGYKGYQEMKIYLAKELVTPMLEIYGEITRRDDLGVV
                     KEKIFQINAQALNDTKKILADSELERAVTAIDQAGRFHIFGLGASGGVAVDAQLKFLR
                     IGIPTVAFTDSHNQTALAALLTKDDAALAITNSGNTKDVVRALAVARAAGATTICLTS
                     SPGSAVVKEAEIKLFTSATETIFRSGAIASRIAQLSVIDVLFIGVALRRYDQSLLCLA
                     KTREAVSDKKY"
BASE COUNT         1176 a         1201 c         1031 g         1065 t
ORIGIN      
        1 tcaataattg tccggccttt tctgcaaggg acatacttgc cacaagctcg gacggtgatc
       61 cgttttggaa aattccattg tggaaagaag tccttacagc tttcttcacg gcatccattt
      121 tgcctcctgt tttcacgata ctgtcctcat cccttgactg cgccggttat cattccctgg
      181 gtgatataac ggcgcacgat gatgaagaat accaatgacg gcagggtgaa cagcaccgag
      241 gcggccatca attgattcca ttccaccgta aattggccga aaaatgcggt caatccgacc
      301 ggcatcgtat ttaatttatc gatattgatg aaagtcaagg caaagacata ctcgttccaa
      361 gcgccgatga aagcataaat ccccgaggcg gcaatccccg gaagggtcag agggagaatc
      421 acccggaaga aggcgcttcc ccaggaacag ccgtcgatca tcgccgcttc ctcaagttcc
      481 ctcggaactc cgtcaaaaaa acccttaagc atccagatac acaaaggtaa ggtgaaggtg
      541 gtataactca ccagcagtga aaggtggtta ttcaataaac ccatattgcg catagtgatg
      601 aagagcggaa tgataattac gaccgaagga aacatctgta ccgaaaggat caggagatac
      661 aagaatttcc gccccggaaa ccgaaaacgg gaaaaaccat atgcccccaa cgcggccagg
      721 gcgataccga tcaaagtcac agtcagggag atataagtac tgtttaaaaa gtagcgcccc
      781 agtaaggtcc ctttgaacgc ttgttcataa ttactcagcg tgaaatgggg cggaatgatc
      841 tttggtactg cggtaaaggt atccgatggc agcttcagtg aagtggataa catccaaaga
      901 aacggaaaaa ccgaaagaac aaccacgact atcgcggcca gataaatccc catatataaa
      961 cccgggtttt tccggtttaa tttcaatttg gccatcagtc cgcctcctct tccctggtga
     1021 agagcttcaa gtagaaaaat gtaatcagca tcaccaatat cagcatgata accgccagtg
     1081 cggaagcgcg acccattcgc agcaaggcga aggcatcgac ccaggtgcga atcccgagca
     1141 acatggtgct gcccgccggt ccgccttcgg tcatgaccca catctggtca aagactttaa
     1201 aatcccaaat cgtggagatc acggtcaaaa tcccgatcat ctcccggagt aacggcagcg
     1261 tgataaagcg tagctgctgc ctaaacccgg cgccgtcaat ctcggcggcc tcataaacct
     1321 caaccggtat ggactgcaag ccggccaaga ggctaacggc gataaaaggg aagctctgcc
     1381 agacgatgac gataatgact gcgagtaacg ccgtcgaggc gttggctaac cacggaaaag
     1441 tggcgaaact tttcaacccc aaagaactta gacaataatt taaaatgccg aattgatcat
     1501 cgtagatcca tttccacaag atggatgagg caaccctcgg catgacccag gggattaaaa
     1561 tcgccactgc caccaatttc ttgccgggaa aatcctgata caaaagtaag gcgactccca
     1621 atcccaccag gatggtcaaa cccacgcaag ccactgtaaa gataacggtt atccaaaggg
     1681 aataccaaaa ctcggggttt ttcagcaaat aaatatagtt ttcaaaacca acatagggcg
     1741 accccttggt cagtacctcg agcagataat atttttgaaa gctgatcaga acgacttgaa
     1801 tgagcgggta taaaattaaa acaccgatgc caattaagga cggtacaata aaggcatagg
     1861 cgatccggtt ttttttgatc cgaccccata gttcattgag aggggaaagc cgtgaagaac
     1921 ggcttacgct ttcggttggc gtcatttagt aatattaccc ccttttacga tcaggtcccc
     1981 tctttccggc tagtccggaa agaggggact gtactgctat ttgtgtacaa attgcaatca
     2041 gttctcgcta tacccgagga tgatgttcat tgccttagcc gcttcggtag tggcttgttc
     2101 cacggttttt ttgcgcatca taatctgttg catcatgatc agcaacgtct tggaattttc
     2161 gatctgcccc cactgcggaa ccgccgggta agtgcgaccg tatttcattt cactggtgaa
     2221 taccgataaa tacggatcat tggcgatttt ggggctctta aacagggaaa gccgcgccgg
     2281 gaagaattta accgtatccg cccaagccag ctgatactcg ggagagagca tcagttgcag
     2341 ataatcccaa gccaggctct tcttcttcga ctgggagaac atcaccagat tactaccgcc
     2401 gatgaagctc gcccgttgtt tggaatacgg caagggagcc acggcaaact tgtctttcag
     2461 ttccggattg gctaaatagt tcgggacaag cggcgccaca tcgatgatca tcgcctgttg
     2521 gccggtttca aaagctttcc ggctggacag cacattccag gtaaccgaac cctcggggct
     2581 taccttggcc ttgagataca gatcggcaaa ccatttgata gccgctctgg cttcgggtga
     2641 atcaatggtg gacttccagc ctttatcggt ctttacggcg atctcccctt tgttcatcca
     2701 aacgagtggg agaaattcgt gcaaggaagc tccgtttacc gaaaaaccgt agcgatcgat
     2761 cttgccgtcg cggttggtat ccttggtcaa tttctttgct accgtggcaa actcatccca
     2821 ggtagtcggg aaactcttga tccctgcttc ggcgaaccag tctttccggt aataaaccac
     2881 gcgattaccg gcataccagg gcattccaaa aagctggcca ttaaatgtag cgctttcaac
     2941 caacgccgga agcagatcct gtttttgacc ccacttggct acgtatgaat ccagatcctg
     3001 aatcgctccc atggaggcaa aatcaggggt ccaggtcgtt ccgacctcgg agatatccgg
     3061 ggccattccc gccacggccg aggttaacag tttctgttgt gcgccggtcc agggaatcca
     3121 agatacattg acggtcgctc cgtcatggtc cttggcgaac tctgcgttca tcttatccat
     3181 gaccattttg gcgccttcgg ggttaccggt ttgcatcaac cagacttcga gggtctcccc
     3241 cgcagccatt ccgatcccgc ttaccagcgc cagactaaga acaaccaaga tgattaagac
     3301 tcgtttcatt tgttctcccc cgtttcgatt ctcttttcga atgctcattc caacgatttg
     3361 attcgcgcgc gtgcgcgcgc cgatcactca cctccccctg caaatcaata ttttttatca
     3421 ctgaccgcct cgcgtgtttt ggcgaggcaa agcagcgatt gatcataacg cctaagggcc
     3481 acaccgataa agagtacgtc aatcacgctg agttgggcga ttcgtgacgc aatggctcca
     3541 ctgcgaaaga ttgtctccgt agccgaggta aataacttga tctccgcctc tttgactacg
     3601 gccgatcccg ggctgcttgt caaacaaatc gtagttgccc ccgcagctct agccacagcc
     3661 aacgcccgga ctacgtcttt ggtatttccc gagttggtta tggccaaggc cgcatcatcc
     3721 tttgtcaaca acgcggccaa tgccgtctga ttgtgtgaat cggtaaaggc aacggtggga
     3781 atgccgatcc gcaagaattt taactgcgcg tcaaccgcca caccgccgga agcgccaagt
     3841 ccaaagatat gaaaccttcc ggcttgatca attgcagtaa cggcacgttc caactcactg
     3901 tcggccagaa tttttttggt atcattcaac gcctgggcat tgatttgaaa tattttttcc
     3961 ttgaccactc ccagatcatc gcggcgggta atctccccat aaatctcaag catcggcgtt
     4021 accaactctt tagccaggta aatcttcatt tcctgatagc ctttgtaacc cacgcgctga
     4081 caaaacttga tgatcgtggc atcactgact ccaacccggt tgctcagttc cgtcaccgaa
     4141 aggtagataa cctcttgggg atgctctaag atataaccag ccactttctg ctctgaaggg
     4201 cgtaaagtgg aatataatgc ccggatcttg atgatgcaag tctccggttc accaaccctt
     4261 tttaacatga agtcattcac tcccgattat tgatcatctg agatgtcagc cggctttccg
     4321 ccgcagaaac cgagcaatca ttctattcct tatttgtgct catcaaacgg ttcaggcatc
     4381 tccgccgtat tcaccaactc cagttcggac cggcatttac ccgaataacc cctcttccag
     4441 aactggttga agttcacctc ggtcacccga ccc
//
LOCUS       DASVMB010000223         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_690355,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000223 DASVMB010000000
VERSION     DASVMB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690355"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1897)
                     /locus_tag="VF531_06605"
     CDS             complement(155..1897)
                     /locus_tag="VF531_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX7713675.1"
                     /translation="MKILLTTLNAKYVHTSLALWYLYQFCKKDHSGLVLREFNINQDL
                     SWVCGEIYLEQADVVAFSCNIWNIEQVLIICRRLKAVAPDTVIILGGPEAAADPRETL
                     AANPAVDFIVCGEGELTFAEWLRELKQSVPRWENISGLAFRSGQGIPVLNEPRPVIPD
                     LALLPFPYPREDLAQFRQKLVYYETSRGCPYHCSYCLSANEREVRCFPMDRVEQELLL
                     LIEAGIAQVKLVDRSFNCNPERAKHIWRFLLAHPGHTNFHFEIVGDLLDEESLAILAA
                     AQPGQFQFEIGVQSTNEETLRLIDRKMDFARLGDRVIRLRRQGRIFVHLDLIAGLPGE
                     DYQSFAKSFNDNMTLAPSRLQLGFLKLLKGSGLREQAADFGYEFTAETPYEVLENNWI
                     SYRELLNLKVIEDLLERYYNSGRFERGFGYLMTRYSSPFQLFEALASWWKGRGYDQVS
                     HREKDLYRYLIEFYPAEGTEGVILRNLLKHDLLRSERMVELPDWAGAITPELRRFSFD
                     FWRNPSNREQFLPELAGLEIRDIQRRVLFGEFDLDPEATWAAIKEGTPQPERKPCIYL
                     FVYGGGTAKTYKVG"
     gene            2105..2959
                     /locus_tag="VF531_06610"
     CDS             2105..2959
                     /locus_tag="VF531_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7713676.1"
                     /translation="MLIDCHTHAFADKIAAKAVEQLITYYKMPTEFGGRLADLMTVAD
                     QAGLDALVLLVAATRPEQVKPANDWALSMLRMTPDQLRQDFFPCSTRALTKVPQLIPF
                     GTFHPNDPNWLEEISRLRAAGIKGIKLHPEFQGIDLADPRLDSFFEEVAPDFILMIHM
                     GDPLVSEANFSTPRKLRHILEQFPKLRAIAAHLGGYCFWEQSYQELAGREVYFDTSSA
                     LAYIDPGMLRRLVSKHGTERILFGSDYPLRAPWQDLELLEQIPWLTAKEKEAISWRNC
                     ARLLDIQL"
     gene            2975..3673
                     /locus_tag="VF531_06615"
     CDS             2975..3673
                     /locus_tag="VF531_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713677.1"
                     /translation="MTGQKAHRRANNTALPLSMPHVYSRFGLPNRVRLLVFFGILVTL
                     FSGFTPAKTASPREQLVGTWLYLWWHGTVSRIQSDNTPTDQLVFLPESYRETDQPQNE
                     TYLRFREDGTGTFFKRYVNIETQRRQTRLTVMTRTMVGTWIPQDTLAVRYEFRYWIQG
                     GKLLIRAAGGAPRRNNSGRYDFTLDLKPERPVSQELVKRQDLGEELFIKQYGLLWKVG
                     AKYDAALRAEYQIP"
     gene            complement(3670..>4003)
                     /locus_tag="VF531_06620"
     CDS             complement(3670..>4003)
                     /locus_tag="VF531_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7713678.1"
                     /translation="NASAKIYELTKLLTEQKINSVPVCNEAGQVIGVVGVSELINLKK
                     KLLVRDIMNRDLPWVDADTPVDEIIPYLEDARLKRLPVFEDGCLVGIISRADILKAMG
                     DNRSPPTN"
BASE COUNT          961 a         1092 c         1011 g          939 t
ORIGIN      
        1 taagttaagt tacacagaat tgataaaaat atgattagcg ccggacctct ctcttaacca
       61 tcatgatgca gtgattttac tgcttcgaac ttaccttctc ccctctttcc cgctcgcggg
      121 agagagggga gcaagagggg tgagtcgctg ttgcctaccc cactttatac gtcttcgcag
      181 tgcctccgcc ataaacaaag agataaatgc aaggtttacg ttccggctgg ggggtacctt
      241 ccttgatggc agcccaggtc gcctcggggt ccaggtcaaa ctcaccgaaa agaacccgcc
      301 gttgaatatc gcgaatctcc agtcccgcca gttccggaag aaactgctcc cggttggatg
      361 gattccgcca gaagtcgaaa ctaaatctac gaagttctgg agtgatggct cccgcccaat
      421 ccggcagctc taccatacgt tcactacgaa gcagatcgtg ctttaacaga ttgcgcagga
      481 tcaccccttc cgtcccttcg gccgggtaga attcaatcag ataccggtat agatccttct
      541 cgcggtgcga aacctgatca tagccccgcc ctttccacca ggatgccaac gcttcaaata
      601 gctgaaaggg actggagtat cgggtcatca ggtatccgaa accccgttca aaccggccgg
      661 aattatagta ccgttccagg agatcttcga tcacctttaa attcagtaac tcacgatagg
      721 atatccagtt gttctccaac acctcatagg gtgtttccgc cgtaaactca tatccgaagt
      781 cggcagcctg ttcccgcagg cccgaacctt tcagcagctt taaaaatccc agttgcagac
      841 ggctgggggc gagagtcatg ttatcgttga aagatttggc gaatgattga taatcttccc
      901 cgggcagacc ggcgattaag tcgagatgga cgaagatcct cccctggcgc cttaaccgga
      961 taacccggtc acccaggcgg gcgaagtcca tcttccggtc gatcaggcgt aatgtttcct
     1021 cgttggtgga ctggactccg atctcaaact ggaactgccc cggttgcgcg gcggccaaga
     1081 tcgcgagtga ctcttcgtca agcagatctc cgacgatctc gaaatgaaag ttggtatggc
     1141 cgggatgggc cagtaaaaac cgccagatgt gcttggctcg ctccgggtta cagttgaagg
     1201 aacggtcgac taacttgacc tgagcaatcc cggcctcgat caaaagcaaa agttcctgct
     1261 caacccggtc cattggaaag caccgcactt cacgttcgtt ggcagacagg caataactgc
     1321 agtgatacgg gcatccccgc gaggtctcgt aatagaccaa tttctgccga aactgggcca
     1381 aatcttcccg gggataggga aaaggcagca gcgccagatc gggaatgacc ggtctgggtt
     1441 cgttcaatac cggaatcccc tggccggaac gaaatgccag accggagatg ttctcccaac
     1501 gggggaccga ttgtttcaat tcccgaagcc attcggcgaa ggttagttct ccctcgccgc
     1561 agacgatgaa gtccaccgcc ggattggcgg cgagcgtttc ccgggggtcg gccgcggcct
     1621 cggggccgcc caatataata accgtatccg gagccaccgc ttttaagcgg cgacagatga
     1681 tgagcacttg ctcgatattc caaatattgc aggagaaagc caccacatcg gcctgctcaa
     1741 gatagatctc cccacagacc caggaaagat cctgattgat gttgaactcc cgaagcacca
     1801 ggccggaatg atccttcttg cagaactggt atagatacca taaagccagt gaagtatgaa
     1861 catatttagc gtttaaggtg gtcaacagta tcttcatgag gtctcccgaa tcggtatcct
     1921 atgtagcgat gatcgctgac agcggcaatc ttttatgcaa accgataggg atagtattat
     1981 ccaaaagact ccggattcct tccttattcc gggaagccga atttttaccc atattttacc
     2041 cgacctttga aagggtttcc ttcccatccg tcgaaacatc atgctaaatt gagaggagtc
     2101 cgatatgctt atcgattgcc atacccacgc tttcgcggat aagattgcgg ccaaggcggt
     2161 ggaacagtta atcacctatt ataagatgcc cacggagttc ggggggcgtc tggcagacct
     2221 gatgacagtg gctgatcaag ccggtctcga tgcgttggta ttgttggtgg ctgccaccag
     2281 gcctgaacag gtcaaacctg ccaacgattg ggcgctctcc atgctccgga tgaccccaga
     2341 ccagctgcgc caggactttt tcccgtgctc aacacgcgca ctcaccaaag tccctcagct
     2401 catcccattc ggtacctttc atccaaacga ccccaattgg ctggaagaga tcagtagact
     2461 tcgtgcagcg gggatcaaag ggatcaagct ccaccccgag tttcaaggga tcgatctggc
     2521 cgatccccgg ttggattcat tctttgagga agtggctccc gattttattc tgatgatcca
     2581 tatgggagac cctctggtca gcgaggccaa tttcagcaca ccccggaaac tccggcatat
     2641 tttagaacag tttcccaaac tccgggccat cgctgcccat ttgggaggct attgtttttg
     2701 ggaacagtcc tatcaggagt tggccggacg ggaggtttac tttgacacct ccagcgcgct
     2761 cgcctatatt gaccccggta tgcttcgccg gctcgtctcc aaacacggca cggagaggat
     2821 cctattcgga agcgattacc cattgcgtgc accgtggcag gatcttgagt tgttagagca
     2881 aatcccctgg ctgaccgcaa aggaaaaaga ggccatctcc tggcgtaact gcgcccggtt
     2941 actcgatatc caactataga ttgggggagt ctttatgacg ggtcaaaaag cgcaccggcg
     3001 cgcaaataac acggctttac cactgtcaat gcctcatgtt tatagccgat tcggtcttcc
     3061 caaccgtgta aggttactgg ttttcttcgg gatcctggtg acgttgtttt caggatttac
     3121 tccggccaaa acagcaagtc cccgggaaca actggtcgga acatggcttt atctctggtg
     3181 gcatggaacg gtcagccgga tacagtccga caataccccc actgatcaat tggttttcct
     3241 gcccgagtcg tatcgggaga cggatcaacc tcaaaatgag acttaccttc gtttccggga
     3301 ggatggtacc ggaactttct ttaaacgata cgtgaatatt gagactcaaa ggcggcaaac
     3361 ccggttgacc gtaatgaccc ggaccatggt aggtacctgg ataccccaag ataccctggc
     3421 ggttcgttat gaatttcgct attggattca gggcgggaag ctcttgatcc gcgcagcggg
     3481 aggcgcaccc cgtcggaaca actccggaag gtatgatttc acattggatc ttaagcccga
     3541 gaggccggta tctcaagaat tagtgaaacg tcaggatttg ggcgaggagt tatttattaa
     3601 gcagtacggc cttttgtgga aggtcggcgc caaatacgat gccgcacttc gggcagagta
     3661 ccagatccct taatttgttg gagggcttct attatccccc atggccttga ggatatcggc
     3721 ccggctgatg atgcccacca gacaaccgtc ttcaaataca ggcaacctct ttaaccgggc
     3781 atcctccaga tacgggatga tctcatccac cggggtatcc gcatctaccc aaggtagatc
     3841 gcggttcatg atatcccgga ccaacagctt cttctttaag ttgatcaatt cggagacccc
     3901 gactacccca atcacttgcc ctgcttcatt gcaaaccggt acgctgttga tcttttgctc
     3961 cgtaagcaac ttggttaatt cgtaaatctt ggccgaagca ttg
//
LOCUS       DASVMB010000224         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_27229,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000224 DASVMB010000000
VERSION     DASVMB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27229"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /gene="thpR"
                     /locus_tag="VF531_06625"
     CDS             <1..314
                     /gene="thpR"
                     /locus_tag="VF531_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX7713679.1"
                     /translation="FPSSKMPRIIWLGLREGGLEIRKLADDVEAACGSCGFPAADRPF
                     QPHLTIARLREGGAVSQAKIPEYQFTSITRVSGFSLIESRLQPAGPVYHSLTDFNLKP
                     S"
     gene            590..1675
                     /gene="recA"
                     /locus_tag="VF531_06630"
     CDS             590..1675
                     /gene="recA"
                     /locus_tag="VF531_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7713680.1"
                     /translation="MSDKEKALEVALLQIEKQFGKGSIMKLGDDSSRMNIEVIPTGAL
                     SLDLALGIGGIPRGRVVEIYGPESSGKTTVALHIAAEAQHLGGIAAFIDAEHALDPLY
                     AKKLGVDIDNLLISQPDTGEQALEIVEALVRSGAVDVIVIDSVAALTPQAEIEGEMGD
                     AHVGLQARLMSQALRKLTAVISKSNTIAIFINQLREKVGVMFGNPETTPGGRALKFYA
                     SVRLEVRKVETIKQGNDVVGSRTKAKVVKNKVAPPFKEGEFDIIYGEGISKAGCVLDM
                     AVDLNIVNKSGAWFSYGESRIGQGRDNARDFLKKDPALLAEIEHKVRASVGLIQEGAP
                     AANTGTAATTGRKDTASAKESASAKGR"
     gene            1699..2139
                     /locus_tag="VF531_06635"
     CDS             1699..2139
                     /locus_tag="VF531_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX7713681.1"
                     /translation="MDAALNRLDRKAYTRRELITQLEKDGFHPDEVAQATERLNGWGY
                     LDDHDFGAERIRILMAREKSRAFVNADLEAKGLAEDLITTLLDEFYPEDREVEIARKF
                     LRKRAVSRNRTPQSVIISLARAGFTPQTIRRCMPEIPESEVSST"
     gene            2451..>3440
                     /locus_tag="VF531_06640"
     CDS             2451..>3440
                     /locus_tag="VF531_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEX7713682.1"
                     /translation="MTAVVGVAVGYFGNVLYGILKLQSAKESARKILDGAKKEVESIK
                     REALLEAKEEALKVKNDLERENRERRADIQRLEKRLLQKEESIDRKYEIVEKKEEALS
                     KKEAEVDRIKGELQEIHQRHRHELERISGLTSEEAKTLLLKDVEEEIQQEIAIKIRDQ
                     EAKAKEESERRAREIISLAIQRCAADHVTEATVSVVALPNDEMKGRIIGREGRNIRAL
                     ETLTGIDLIIDDTPEAVILSGFDPVRREVARLALEKLIADGRIHPARIEEMVEKAQKE
                     VEATIKEEGERATFETGIHGLHPETIKLLGRLKFRTSYGQNVLQHSIEVSHLCG"
BASE COUNT          920 a          771 c          953 g          796 t
ORIGIN      
        1 tttttccctc ctccaaaatg ccgcgtatca tctggcttgg gttgcgagag ggggggcttg
       61 agatccgaaa attagcggat gacgttgagg cggcctgcgg cagttgcggt ttcccggcgg
      121 cagatcgccc ttttcaacca cacctgacca ttgccaggct ccgggaaggc ggggccgttt
      181 ctcaggcgaa gataccggaa tatcaattta ccagtatcac ccgggtctcc ggtttttcct
      241 tgatcgaaag ccggttgcaa cccgcgggcc cagtctacca ttcgttgact gactttaatt
      301 tgaaaccttc gtaaaaagtt taccgtaaat tctttgccgg attcaggggc cggtttcaga
      361 gtgttacaaa tgtctcttag cgtctgttta tatagtgaat atggaatttc ggaccggaaa
      421 ttttcatgac ctattgccaa gcgcgccaag gtttgttata cttaatcaga acacatgttt
      481 ggtttgccga actgtaatct gaatgtctta aaatatttgt caatccacgg cgggatctgt
      541 atcgtgtaag ttcaagtttg cgccgccact tgttgaggag gggttcggga tgtccgataa
      601 agagaaggct ttggaagtcg ccttgttgca aatcgagaaa caattcggaa aaggttccat
      661 catgaagttg ggcgacgatt cgtccagaat gaatattgaa gtgatcccca ccggggcact
      721 ttcgttagat ttggcgctgg ggatcggcgg tattccccgg ggcagggtgg tcgagatata
      781 cggccccgaa tcctccggta agaccacagt ggcgttacat atcgcagcag aggcccagca
      841 tcttggggga attgccgcat ttatcgatgc cgaacacgct ttggatccgc tatatgccaa
      901 aaaactgggt gtcgacatcg acaatctgct tatttcccaa ccggacaccg gtgaacaggc
      961 tttggagatc gtcgaagcct tggtgcgcag cggagcagtt gatgttatcg taattgattc
     1021 ggtggccgcc ttgactccgc aggctgagat cgagggggag atgggcgatg cccacgtggg
     1081 attacaggca cggttgatgt ctcaggcgct ccgcaaatta acggcggtga tcagtaaatc
     1141 aaataccatt gcaatcttta tcaaccagct ccgtgagaag gtcggagtga tgttcggcaa
     1201 ccccgaaacc accccgggcg gccgggcctt gaagttttat gcctcggtcc ggctggaagt
     1261 gcgtaaggtg gaaacgatca agcagggaaa cgatgtcgtc ggaagtcgca ccaaggccaa
     1321 agtggttaag aataaagtcg cccctccgtt taaagagggc gaattcgata ttatttacgg
     1381 cgaagggatc tccaaggccg gttgcgtctt ggatatggct gtcgatttaa acatcgttaa
     1441 caagagcggt gcttggttct catacggtga gagccggatc ggtcaaggcc gggataatgc
     1501 cagggacttt ctgaagaaag atccggcgct cttggcggag attgaacaca aggtacgggc
     1561 ctcggtcgga ctgatacagg agggagcacc ggcagccaat accgggactg ccgccacaac
     1621 gggtcgcaag gataccgcct cagccaaaga atctgcctcg gcaaagggga gataaatacg
     1681 aagtcggcca ataacgcaat ggatgcagct ctcaatcgcc tggaccggaa agcctataca
     1741 cgccgggagc taatcaccca attggaaaag gacggttttc atccggatga ggtagctcag
     1801 gctactgaac ggttaaacgg ttgggggtat ctggacgacc atgatttcgg agccgagcgg
     1861 atccggatct taatggcaag agagaaaagc cgggcctttg taaacgctga tctggaggct
     1921 aaaggattgg ccgaggatct aatcaccacc ctattggacg agttttatcc cgaggatcgg
     1981 gaagtggaga tcgcaagaaa gtttctgagg aagcgcgctg tttccaggaa ccggacgccc
     2041 caatcggtca tcatttcatt ggcccgggcc ggttttaccc cccaaaccat ccggcgatgc
     2101 atgccggaaa tccccgaatc tgaagtatct tctacttgac accgtattta aaaaagttta
     2161 caataaactc ttgaggacag aacataagcc gagttctact cggcttgttt tgtatttatt
     2221 tttcggtgaa tcgctatttt ccaaactcaa gcagttttat aattttcgcc tcgtactcac
     2281 cggtttacac ccaattgtca ttcattgaac ctctccggtt tccggggcgt ccgaaattgt
     2341 aaaattggac cagttatcgc aagcgttcaa aagtaaatgt aagtgatgga atgaaataag
     2401 tcggatcaaa tgatcaggag gtgattcgta taggagcatt acccattttc ataacggctg
     2461 tagtaggcgt tgccgtggga tatttcggca atgtgcttta tggaatcctt aaactccagt
     2521 cggccaagga gtcagccagg aagatactgg atggagctaa aaaagaagtt gaatccatca
     2581 aacgcgaggc tttactggag gccaaggaag aagctttaaa ggttaaaaac gacctcgaac
     2641 gggaaaaccg agagcgtcgt gctgatattc aacgtttgga aaagcgatta ctgcaaaaag
     2701 aggaatctat cgatcgaaag tacgagatcg ttgagaaaaa ggaagaagcc ctctccaaaa
     2761 aagaggctga agttgatagg atcaaaggtg agttacaaga gattcatcag cgtcatcgac
     2821 acgaattgga acgaatctcc ggtttgacaa gcgaagaggc gaaaacttta ctcctcaagg
     2881 acgtggaaga agaaatccag caggagattg ccattaagat tcgtgatcag gaagccaagg
     2941 ccaaggaaga atcggaacgg agggcacggg agattatttc ccttgcgatt caacgttgcg
     3001 cggcggatca tgtgaccgaa gccactgttt ctgtagtggc attaccgaac gacgagatga
     3061 agggaagaat tatcggacgt gagggccgga atattcgagc cctggagaca ttaacaggta
     3121 ttgatttgat catcgacgac acaccggaag cagtcattct ttccggcttt gatccggtac
     3181 gacgggaagt agcccgcctg gctctggaaa aattgattgc cgacggacgg attcatccgg
     3241 cccggatcga agagatggtt gagaaagccc agaaagaggt ggaggcgacc atcaaagaag
     3301 aaggtgaacg ggccacattc gaaacgggaa ttcacggcct tcatccggag actatcaagt
     3361 tgttggggag actgaaattc cgtaccagtt acggacagaa tgtgttgcaa cactcaattg
     3421 aagtctcgca tctttgcgga
//
LOCUS       DASVMB010000225         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_27382,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000225 DASVMB010000000
VERSION     DASVMB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27382"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF531_06645"
     CDS             complement(<1..498)
                     /locus_tag="VF531_06645"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HEX7713683.1"
                     /translation="MSKTATIYYEKISSLLKEIVENEQDSIDRGASILAKCVMDDGLI
                     NVIGPGGHSNLAAEEVLWRAGGLAPVNPILDPGTSLFFGAKRSNYIERTPGYAKAVLD
                     SYEVTEGDVLIIVNAYGINAMTIDCALECKKRGVTSIGLTSTSFADSVPKGAVSRHPS
                     GQNLYE"
     gene            complement(522..1754)
                     /gene="nagA"
                     /locus_tag="VF531_06650"
     CDS             complement(522..1754)
                     /gene="nagA"
                     /locus_tag="VF531_06650"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7713684.1"
                     /translation="MNDLVALTNGTVITPVREIKNGTVLIQQGLIKEVGPRELLDLPE
                     TTEVIDVKGAYISPGFIDLHLHGAGGGDVMAASDHDLELMSLGVAKGGVTSFLPTTLS
                     GSLDSIVQAVNCISRAMSRGVSGAKILGAHLEGPYFNIKQCGAQNPRFIINPKPEDYL
                     PILDRYPCILRISAAPELPGALELGQELRHRGIVASIGHSDATYQEVVRAVENGYTHV
                     THIFSGMSGLKRIEAYRVSGLIESTLLLDELTTEMIADGQHLPPSLMRLVLKTKSLDR
                     VCLVTDSMAAAGLGSGHYQLGGLDVIVEANIPDVFEIPTKENNFVAKLTDRSAFASSV
                     ATMDQLIRNMVEFVGLSIADAVRLVTYNPAKMQGLEDEIGIIASGKKADLTVFDKEIN
                     IELTMVEGKVVYNKLYSI"
     gene            complement(1810..3048)
                     /locus_tag="VF531_06655"
     CDS             complement(1810..3048)
                     /locus_tag="VF531_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7713685.1"
                     /translation="MMVFSQSPNNSRGLKVLNRILVLDAIRELGPIARYEVAKVTSLT
                     PPTVTVIVNDLIKAGIVEEVGSGESSGGRKPVLLELDPHSGYILAVRLQHGEIMTALL
                     DLTGNVLEDRGCRLDTSSPETVVTAIEESFVSFLERLRIAPEKTLFCGVASPGLIDHN
                     NSMIDRSSNLGWKKVPLGQMLSPRLAGIPVHIENISNAAALGEKVYGSGRGAADLIYL
                     NLSIGIGAGIIINNEVYNGAQGYAGEVGHMVLLPENGPKCSCGQYGCFEAVCGVRTVL
                     SRIRAEVPDEVFARLGISKIRCGILDLVNPLLLKTPEVQNILADTGKFIGIAIANLVS
                     LFNIPTVILGGELALLGGTILDTVTEKVKECTLPEIGESVRVICSTMNEDPPLMGVYA
                     SVLEKVFSTDQWLFKKLNDL"
     gene            3477..>3807
                     /locus_tag="VF531_06660"
     CDS             3477..>3807
                     /locus_tag="VF531_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713686.1"
                     /translation="MDQSDMMRPVQFSDTYYYGIRPTISKVAAEMDDMLVAYPTQAMY
                     DSMLDRVYGEMAKIYPEEMQDQLSNPTLNSPDYHAEQFFPPFRRRRFVRDLLGILLLE
                     ELFERRRR"
BASE COUNT         1064 a          973 c          743 g         1027 t
ORIGIN      
        1 ctcataaaga ttttgcccgc tcggatgacg ggataccgct cctttcggaa cgctgtcggc
       61 aaaggaagtt gaggtgagcc cgatggaagt gacccccctt tttttgcatt ccaaggcaca
      121 atcgatcgtc attgcattaa ttccatacgc attgacgata atcaggacgt ccccctcggt
      181 cacttcatac gaatcaagta cggctttcgc gtatcccggt gtcctctcga tatagttgga
      241 acgttttgcg ccgaaaaaca aactggttcc gggatccaaa atcggattaa ccggagctaa
      301 accgccggcg cgccataaaa cttcctcagc ggctaaattg gaatgtccgc cggggccaat
      361 gacattgatc aggccatcat ccatcacgca tttagctaaa attgacgcgc cgcgatcaat
      421 ggaatcttgc tcattctcta caatctcttt tagtaaagaa gagatttttt cataatagat
      481 cgttgctgtt tttgacatcc aaaatcgctc cttttgttag attaaattga gtataattta
      541 ttataaacaa ccttaccttc caccattgtc agttcaatat ttatttcttt atcaaatacc
      601 gtcagatcag cctttttccc gctggcgatg atgccgattt catcctctag tccctgcatt
      661 ttggcgggat tataggtcac cagcctaacg gcatcggcaa tactcaaacc cacaaattcc
      721 accatattgc ggatcagttg atccatggta gccacactac tggcaaaagc gctacgatcc
      781 gtaagtttgg caacaaaatt attttctttg gttggtatct cgaaaacatc gggaatatta
      841 gcctctacaa tcacatcaag cccacccaat tggtaatggc ctgagcccaa gccggcggca
      901 gccattgagt cagttaccag gcatactcga tccaaacttt tcgtctttaa aaccaacctc
      961 attaaactcg gcggtaaatg ctgcccatcg gcaatcatct ctgttgttaa ttcgtcaagt
     1021 aggagtgtgg attcaataag cccggaaaca cgatacgcct cgatcctttt taaacctgac
     1081 ataccggaaa aaatatgggt tacatgggta taaccatttt caaccgccct gactacttcc
     1141 tgataggtgg catcggagtg accaatcgag gctacaattc ctctatgcct taattcctgt
     1201 cccagctcaa gcgcaccggg caactctggt gcagcggaga tacgcaggat acagggatac
     1261 ctgtccaaaa taggcaaata atcctccggc tttggattga taatgaaacg gggattttga
     1321 gccccacact gctttatatt gaaatacggg ccctcaagat gggcacccaa aatttttgca
     1381 ccgctcaccc cccggctcat tgcccgggat atacaattca cagcttgaac aatactatcc
     1441 agactgcccg acagggtggt gggaagaaaa gatgtgactc cacctttggc cacacccaga
     1501 ctcattaatt ccaagtcgtg gtctgaagcg gccatgacat cgccgccccc cgcgccgtgc
     1561 aggtgaagat cgataaatcc cgggctgata tacgcccctt taacatcgat tacttccgtc
     1621 gtttccggta gatctaacag ttccctcggc cctacttctt taatcaaccc ctgttgtatc
     1681 agaaccgttc catttttgat ctcccggaca ggagtaatca ccgtcccgtt cgttaaagca
     1741 accagatcat tcatccattc attactcctt aatcagataa ttacggcact tatccaacaa
     1801 aacaagagtt tacagatcat ttaacttttt aaacagccat tggtcagtag aaaaaacttt
     1861 ctccaatacc gatgcataca ctcccataag cggcgggtct tcattcatag tggaacaaat
     1921 aacccgaacg gactctccaa tctccggcag tgtgcattcc ttaacctttt cagtcaccgt
     1981 atccaagatg gtccccccta acagggccaa ttcgccgccg aggattaccg tgggaatatt
     2041 gaataaactt accagattgg caatagcaat gccgatgaac ttgccggtat cagccaggat
     2101 attttgtacc tccggcgttt tcaaaagtaa aggattgact agatccaaaa taccgcaacg
     2161 tattttagaa attcccaagc gagcaaatac ttcatctggt acttcggcac gaattctgct
     2221 cagaacagtc cggactccgc aaaccgcttc aaaacatccg tattgtccac aagaacactt
     2281 ggggccgttt tcaggtaata agaccatatg accgacttct ccggcataac cttgcgcgcc
     2341 gttatatacc tcgttgttaa taataattcc ggctccaatg ccgatcgata agttcaagta
     2401 aatcagatcg gccgcgccgc gcccgctgcc gtatactttc tctcccaagg cggcagcgtt
     2461 tgagatattt tcaatgtgta ccggaatccc agctaatcgt ggagataaca tttgacctag
     2521 cggaaccttt ttccaaccca agttggaaga acgatcaatc atggaattgt tatgatcgat
     2581 taatcccggg gaagccaccc cgcaaaaaag agttttttct ggggctatcc gaagccgctc
     2641 aagaaatgag acaaaagatt cttcaatcgc cgtaactact gtttccggtg aagatgtgtc
     2701 caaccggcag ccgcggtctt ctaaaacatt tccagtcaaa tcaaggagcg ctgtcataat
     2761 ctcgccgtgt tgcaatctga cagctaaaat atatccggaa tgaggatcaa gttccaataa
     2821 tactggttta cggcctccac ttgattcgcc cgatcctact tcctcgacga tacctgcctt
     2881 gatgaggtca ttgacaatca cggtcaccgt cggtggagtc aaactagtga ctttggctac
     2941 ttcgtaccgg gcaatcggtc ccaattccct aattgcatcc aaaaccaata tgcggtttaa
     3001 aacctttaaa ccccggctgt tatttggaga ctgtgaaaaa accatcatta tcctccatcc
     3061 aaaagtttaa aaaccaaatt ttgggttcgg ggaaattgat acatccgctt cgatttttca
     3121 aactaactca aatgaaataa ctttgttttt ttatctaaaa aagtaattcg ctatgatcct
     3181 ttatattcct gcattgcctg tcaattattt ataactatat ctctatgatt ttatcatcct
     3241 gccattaacc ccgagcccag atcactaaag cattataaaa tagctgttac tttttttatc
     3301 taaaaaagta tactctttgg ctaggatcaa aatttctaca atttccccgc cccagtgaga
     3361 ccttgaatgg ctccgaaata agcaatagtc ccaattcaaa agtctcacct atcaaaagtc
     3421 cttacataaa ctattttgaa aaacatccca cccattgaat ggaggtattg atataaatgg
     3481 atcaatccga tatgatgcga ccggtacagt tttccgatac ttattattac ggaattagac
     3541 ccaccatcag taaagtcgcc gcggagatgg atgatatgtt ggttgcttat cccacccagg
     3601 caatgtatga ctcgatgctg gaccgggtgt acggcgaaat ggcgaagatt tatccggagg
     3661 aaatgcaaga ccagttatcc aatccaactc ttaacagccc cgattaccat gcggagcaat
     3721 tctttccccc atttcgcaga agacggtttg tcagagattt gcttggaata ttactcctcg
     3781 aggagttgtt tgaacggcgg cggagat
//
LOCUS       DASVMB010000226         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_27433,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000226 DASVMB010000000
VERSION     DASVMB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27433"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF531_06665"
     CDS             <1..230
                     /locus_tag="VF531_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713687.1"
                     /translation="LGNMLNVGFEKIILLYNPAIYETADVISSFVYRKGIQEFNWGFS
                     TAVGLFNSVFNFILLLIANWTSKKVKDTALW"
     gene            243..1121
                     /locus_tag="VF531_06670"
     CDS             243..1121
                     /locus_tag="VF531_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713688.1"
                     /translation="MHQENIGEKLFNYFNIGLMTLFSFVTLYPILYVAFASVSEPNEL
                     MGHLGPLWKPLGLHFGSYLLVFKNPNIITGYLNTIFLVTVGVIVNVLMTMIGAYFLSR
                     KNVMWRDLIMFLIVFSMFFSGGLIPFYLIVKGLHINNTLWALILPVAVNTFNLIIMRT
                     QFASIPESMEESAVIDGAGHFTILFRIIVPLSLPTVAVIVLYYAVSHWNAWFNAMIFI
                     QKRELYPLQLILREILIQNDTNTMIQNINTEEQQMVSETVKYAVIIVATLPILVLYPF
                     LQKYFTKGVMVGAIKE"
     gene            1212..4358
                     /locus_tag="VF531_06675"
     CDS             1212..4358
                     /locus_tag="VF531_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713689.1"
                     /translation="MNLYPKNATPKLTEELFRNPSAEYRGTPFWAWNCRLDKELLLKQ
                     IGQLQAMGMGGFHIHSRTGMATPYLGNEFMELVRLCHEEAKRRNMLCWLYDEDRWPSG
                     AAGGLVTKEKRFRSRYLVFMPGEGDSLYCPTKQDFDRMLQNGANPRGYFLAKYGVKLE
                     DGVLVNYQRITDGISSADGMKIYRAYLEISPDKPWHNNQAYVNTLDREAIERFVELTH
                     EQYFRHFGADFGKTIPAIFTDEPQFHHKESFQFATEERTVILPFSDDLPDTYLAEYGM
                     DLLDHLPELFWELPDGKVSVARYRYHEHVAERFTTAFADTVGAWCSSHGIMLTGHMME
                     EPTLFSQTKALGEAMRSYRSFQLPGIDILCDRREYTTAKQAQSAAHQYGCPGVLSELY
                     GVTNWDFDFRGHKLQGDWQAALGVTVRVHHLTLVSMAGEAKRDYPASIGYQSPWYKEY
                     HLIEDYFSRVNTALTRGVPRVRIGVIHPIESYWLHWGPLEQTFAVRDELENNFRNITE
                     WLLFGLLDFDYLSEALLPSLTPGEVSVPFEAGKMKYDVIIVPGCHTLRSTTVARLEAF
                     QKAGGVVIFSGEPVWLVDAEPSDRVRQLAAKCSVVPFTKRRITEALEPYREVDIRNSD
                     GSRTEDLIYQMRNDGGSRWLFICHARTVEDPWGYKLRDQPSFKPQKIEIRLKGKWRPS
                     IYNGMDGTICLADARVRGNDTVINHEFYSHDSLLLRLEPGEAAIGYSSPLKLELPADP
                     VYMADPPAITLDEPNVLLLDMAEYSFDGGAWQPVEEILRIDTLFRKQLGYKIKTEPMA
                     QPWTDTAVEPPSHTLSLRFRVQSEIEIPFPSLALENPENTLIIVNGKEVQPIVKGWFV
                     DEAIKTVSLPALVKGENEIILNIPFGARTDVEWCYLLGDFGVEVQGRFAKIIKSPRTL
                     AFGDWTEQGLPFYAGNVTYHCPIHSHGGNLIIQVPLFQNTVLGIKLDGKTKGDIAFEP
                     YTLNLGYVEAGEHQLEITAFGNRVNAFGAVHNCIRNFHWYGPNAWRSTGVGWSYQYRL
                     RPMGVLVTPRIFNIIEGC"
     gene            4394..>5144
                     /locus_tag="VF531_06680"
     CDS             4394..>5144
                     /locus_tag="VF531_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7713690.1"
                     /translation="MSLNGPAGNNVAKQRLTPLQWAEKACETLMSKYPPQELPPVGRF
                     HYHQGVFLSGMEKCWKETQNEKYYEYIKKWVDSIIFPDGSIHDYNPDELDDIQPGILL
                     FNLYERTGDERYFKALSTLVSLLRTWKRNSEGGFWHKTRHPNQMWLDSLFMGGPIAVQ
                     FGRKFKDDFFCDVAVEQALLIERHTLDEKTGLLYHGWDESKEAAWADPETGKAPEFWG
                     RAIGWYPAALLDMLEYLPEDHKERGKLVEILK"
BASE COUNT         1341 a         1072 c         1302 g         1429 t
ORIGIN      
        1 ggctcggcaa catgcttaat gtcggttttg aaaagattat cctcttatat aatccggcaa
       61 tttatgaaac tgccgatgta atctcctcat ttgtttatcg aaagggcatt caggaattta
      121 attggggatt tagtaccgcc gtgggtttgt tcaattcggt gttcaatttt attttactac
      181 tcatcgccaa ctggacttca aaaaaagtta aagataccgc tttatggtaa gggggttcga
      241 ttatgcatca ggaaaatatc ggtgaaaaac tattcaatta ctttaatatc ggcttgatga
      301 cccttttttc tttcgtcact ctttatccaa tattatatgt ggccttcgcc tcggtgagcg
      361 aaccgaatga actcatgggg catttagggc ctttgtggaa acctttgggt cttcatttcg
      421 gctcttatct attggttttt aaaaacccca atattataac cgggtatctg aacactattt
      481 ttttggttac ggttggggta atagttaatg ttctaatgac gatgatcggg gcctattttt
      541 tatcccgcaa gaatgtaatg tggcgggatc tgattatgtt cctaatcgtt ttttcgatgt
      601 ttttcagcgg cggcttaatt cccttctatt tgatagtaaa aggattgcat attaacaaca
      661 ctttatgggc attaatcctc ccggtggcgg taaatacatt caatttaata atcatgcgga
      721 ctcaattcgc ctctattccg gagagtatgg aggaatcggc cgtaatcgac ggagccggcc
      781 attttacgat tttgttccgg attatagtcc cgttatcctt gccgacagtc gccgtgattg
      841 tattgtatta tgcagtcagt cattggaacg cctggtttaa tgcgatgatt tttattcaaa
      901 aacgggaact gtatccgttg cagttaattt tgagagaaat tttgattcaa aacgatacca
      961 atacgatgat ccagaatatc aatacggaag aacaacaaat ggtgagtgaa acggtaaaat
     1021 atgcggtcat tattgtggct actctgccga ttttagtgtt atatccgttt ttacagaaat
     1081 attttaccaa aggagttatg gtgggcgcta taaaggaata atccggtttt tggttacgga
     1141 atccgagcaa ataatgactt attgtcaggt gcgcttaatg ctgtttatgc ttaaaaatgg
     1201 agttgagatc gatgaatcta tatccgaaga atgcaacccc caaattaacg gaggagttgt
     1261 ttcgcaatcc atccgccgaa taccggggta ccccgttctg ggcttggaat tgtcgcttgg
     1321 acaaggaact attgttgaaa cagatcggcc agttacaagc gatggggatg ggcggatttc
     1381 acatccatag ccggaccggg atggccactc cttacttggg taatgagttt atggaattgg
     1441 taaggctatg ccatgaggaa gccaaacgtc gcaatatgct ctgttggctc tatgatgaag
     1501 atcgttggcc gtcgggggct gccggcggtc tggtcactaa ggaaaaacgc ttccgttcaa
     1561 ggtatctggt atttatgccc ggcgagggcg attctttgta ctgccccacc aaacaggatt
     1621 ttgatcggat gttacaaaac ggcgctaacc cgcgcggtta ttttttggct aaatacggag
     1681 tgaaattgga agacggtgta ttggtaaatt atcagcgaat aacagatgga ataagttccg
     1741 ccgacgggat gaagatctac cgggcttatc ttgagatttc ccccgataaa ccctggcata
     1801 acaatcaagc ttatgtaaac accttggatc gggaggccat cgaacgcttt gttgagctca
     1861 ctcatgaaca atattttcgc catttcggcg cagatttcgg taaaactatt cccgctattt
     1921 tcaccgatga gcctcagttt catcataagg aatcgtttca atttgccact gaagaacgaa
     1981 cggtgatttt accgtttagc gacgatttac ccgatacata cctggccgag tacggaatgg
     2041 atctcttaga tcatttgccc gaattgttct gggaacttcc tgatggcaaa gtttcggttg
     2101 cccggtaccg ataccacgaa catgtagcgg aacggtttac gacggcattc gccgatacag
     2161 tcggggcatg gtgctcgtcc cacggcatta tgctaaccgg gcacatgatg gaagaaccga
     2221 cgctattttc ccagactaag gcgttgggag aagcaatgcg ttcttatcgt tctttccaac
     2281 ttcctggaat tgacatccta tgtgaccgta gagaatatac cactgctaaa caagctcaaa
     2341 gcgccgcgca tcagtatgga tgccccggtg tattaagcga attgtacggg gtgaccaatt
     2401 gggattttga tttccggggt cacaaattac agggcgactg gcaggccgct ttgggtgtga
     2461 ctgtgagagt gcatcattta accctcgtat cgatggccgg ggaagcgaaa cgtgattatc
     2521 cggccagtat tggatatcaa tctccatggt ataaagaata tcacctgatt gaggattatt
     2581 ttagcagggt gaacacagca ttgacccgtg gtgtgcctcg ggtccggatt ggcgtgattc
     2641 atcccatcga atcatactgg ctgcattggg ggcctttgga acagactttt gcggtcagag
     2701 acgaactgga gaataatttc cgtaatatta cggaatggct gttatttggg ttgcttgact
     2761 ttgactatct ctccgaggct cttttgccat cactgactcc cggtgaggtc tcagtcccgt
     2821 ttgaggccgg aaagatgaaa tatgatgtta ttattgtgcc gggatgtcat acgctgcgtt
     2881 cgacaaccgt agccaggctg gaagcttttc aaaaagccgg cggagttgtg atttttagcg
     2941 gcgaacccgt ctggctggtt gacgcggagc cgtccgatcg ggtgcggcaa ttggcggcga
     3001 agtgttcagt agtacccttt accaagagac ggattactga ggcgcttgaa ccctaccggg
     3061 aggttgatat tcgcaattct gacggttccc ggacggaaga cctcatttac caaatgagga
     3121 atgatggagg ttcccgttgg cttttcatct gtcatgccag gactgtcgaa gatccttggg
     3181 gttataaatt acgcgatcaa ccgtcattta agccgcaaaa gatcgaaata aggttgaagg
     3241 gcaagtggcg cccgtcgatt tacaatggca tggacggaac gatttgcctt gccgacgccc
     3301 gcgtcagagg aaatgatacc gttattaatc atgagtttta cagccatgac agcttattgc
     3361 tccgcttgga acccggggag gccgccattg ggtactcgtc ccctcttaag ctggaattgc
     3421 cggcagatcc ggtttatatg gcggatccgc ccgccattac cctggatgag ccgaatgtct
     3481 tgttgttgga tatggccgaa tactcttttg acggcggcgc ttggcaaccg gtcgaggaga
     3541 tattgcggat cgacaccctt tttcgaaagc aattgggtta taaaataaaa accgaaccca
     3601 tggcccagcc ttggacggac actgccgttg agccgccttc ccatactttg agcttacgat
     3661 ttcgggttca atccgaaatt gaaattccat ttccatcctt ggcattagaa aatccggaaa
     3721 acactttgat aatcgtcaac gggaaggaag ttcagccgat tgtcaaaggc tggtttgtcg
     3781 acgaggccat taaaacagtt tccctaccgg cgctcgttaa aggtgaaaat gagattatcc
     3841 tgaatattcc ttttggcgcc aggaccgatg ttgaatggtg ctatctactt ggggatttcg
     3901 gtgtggaagt tcagggacgg ttcgccaaaa tcatcaaatc cccccgcact ctggcctttg
     3961 gtgactggac tgaacaaggt ttgccttttt acgccggaaa tgtaacctat cattgcccga
     4021 ttcattcaca tgggggcaat ttgataatcc aggttccgtt gtttcagaat acggttctag
     4081 ggataaaact ggacggtaag acaaaagggg atattgcttt tgagccgtat accttaaatc
     4141 tggggtatgt ggaagcaggg gaacatcaac ttgaaataac cgccttcggg aaccgggtca
     4201 atgcttttgg cgcagtccac aattgtattc gtaattttca ttggtatgga cctaacgctt
     4261 ggcgaagcac cggagtgggc tggtcttatc agtatcggtt aagaccgatg ggtgtccttg
     4321 taaccccacg aatattcaat atcatcgaag gctgctgaaa agaaaaccgt cagataagga
     4381 gtggcataag aaaatgagtt taaatggacc ggcaggcaac aacgttgcca aacagcgact
     4441 gacaccgtta caatgggctg aaaaagcctg tgaaacactg atgtcaaaat acccgccgca
     4501 ggaattgccc ccggttggca ggtttcatta tcatcagggc gtctttttgt cgggcatgga
     4561 gaaatgttgg aaggagaccc aaaacgagaa gtattatgag tatatcaaaa aatgggttga
     4621 tagcatcatc ttcccggacg gaagcattca tgattataac ccggacgaat tggacgatat
     4681 ccaacccgga atacttctgt tcaaccttta tgaacgaacg ggagatgaac gttatttcaa
     4741 ggccttgtcg acactggttt ctttgttgcg tacctggaaa agaaatagcg aagggggatt
     4801 ttggcataaa acaagacatc cgaatcagat gtggctggat tcgctcttta tgggaggacc
     4861 gattgcggtc caattcggca gaaaatttaa agatgatttc ttttgtgatg ttgcagtcga
     4921 acaggcttta cttatcgaga gacatacgtt agatgaaaaa accggattgt tatatcatgg
     4981 ctgggacgaa agtaaggaag cggcatgggc ggatccggag accggaaagg ctcctgaatt
     5041 ttgggggcgg gcgattggct ggtatccggc agcactcctg gatatgctgg aatatctccc
     5101 cgaagaccat aaagaacgag gaaaattggt ggagatttta aaga
//
LOCUS       DASVMB010000227         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_173296,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000227 DASVMB010000000
VERSION     DASVMB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173296"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..384)
                     /locus_tag="VF531_06685"
     CDS             complement(10..384)
                     /locus_tag="VF531_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7713691.1"
                     /translation="MEKETGIGKNNQDVCDSFCPSDANLDGLREKVLEVAGLSEIFKV
                     LGDETRTKILYLLAHRKLCVCDIAVILEMSLPAVSHHLRLLKALRLVKYARDGKMVYY
                     SLDDEHILNLIREAQEHFAEGR"
     gene            complement(560..1192)
                     /locus_tag="VF531_06690"
     CDS             complement(560..1192)
                     /locus_tag="VF531_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014726301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEX7713692.1"
                     /translation="MDKKPTSETYTEFQTAYDFFNRELFGSQLPDCLITYQRKAKTKG
                     YFADGRFENTENGRIIDEIAMNPDYFSGESISSKTILSTLVHEMCHLWQKYYGKPGRN
                     RYHNQEWAEMMLKVGLLPTDNKTPTGKMTGDKMSHLIIKGGAFDRVCDRLLENIKIAW
                     VDRWTGMDSQSGSKLKNKTKYVCPVCHLNVWGKPGIKILCGNCMCKLVEE"
     gene            complement(1341..>2642)
                     /locus_tag="VF531_06695"
     CDS             complement(1341..>2642)
                     /locus_tag="VF531_06695"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX7713693.1"
                     /translation="YESHKLVTYPRTDSRYLSEDIVPTLPERLRSVADAGPYAESVQA
                     ILKSKIVPTKRLVDNAKVTDHHAIIPTEEPVYLSKLNSEEFRIYDLIVKRFLAVLSPA
                     FEYQETTLQVGVNGEVFTAGGKTVKLQGWRRIYQNLGNLEEEADFDDSGDPTGRETAQ
                     TLPEFHEGEVIKIQSVHMAAGKTKPPARYTEATLLTAMEHPGKNIQDHGLREALENSS
                     GLGTPATRAEIIEKLFNSFYMERHGKEIIPTSKGVQLIGLVPPELKSPELTAKWERQL
                     SEIGKGRADHTDFVKEIRNYTVQLVGAVAGSSQTYRHDNLTRAKCPDCGKYLLQVKGK
                     HGEMLVCQDRSCGYRESISVHSNARCPQCHKKMELRGEGEGKIFTCSCGYREKLSAFT
                     KRRETEKAGANKREVQKYLHNQSDDAPINTALAEALAKLKQ"
BASE COUNT          625 a          655 c          586 g          776 t
ORIGIN      
        1 tcaataaatt tatcttcctt cggcaaaatg ctcctgcgct tcgcggatca aatttaatat
       61 gtgttcatcg tctagagaat aataaaccat tttaccgtcc cgggcatatt tcaccaaccg
      121 tagcgccttt aaaagtcgta aatgatggga taccgccgga agactcattt ccaaaatcac
      181 ggcaatatca cagacgcaaa gcttacgatg ggctaaaaga tataatatct tggttcgggt
      241 ctcgtcgccc aaaaccttaa aaatctccga taatccggct acttctagaa ccttttcccg
      301 taaaccgtca aggttggcat ccgaaggaca aaaactatcg catacatcct ggttgttctt
      361 gcctataccc gtctcttttt ccatggtaca cctccatata acgattaagc aattgcttaa
      421 ttgtaattat aaaaaaactc tccccaattg tcaagtttat caaaataaat accttgagtt
      481 gatcaggtgc tctttttggt acggatagat gtatgtttca tcaacgattt tgccgtttcg
      541 gataatcaat accctcggtt cactcttcaa ccaacttaca catgcaattt ccacaaagaa
      601 ttttgattcc cggtttaccc caaacattca ggtgacacac agggcagaca tatttggtct
      661 tattcttcag ttttgaacct gattgtgaat ccatgccggt ccagcgatcc acccaggcga
      721 tcttgatgtt ctcaagtaat cgatcacaaa cccgatcgaa agccccgcct ttaatgatca
      781 gatgagacat tttatctcct gtcattttcc cagtcggagt cttgttatcg gtcgggagta
      841 aaccaacttt tagcatcatt tcagcccatt cctggttgtg ataccgattc cggcccggtt
      901 tgccgtaata tttctgccaa agatgacaca tctcgtgaac cagggtggat aatatggttt
      961 ttgaagaaat gctctccccg ctgaaataat cgggattcat ggcgatttca tcgataatcc
     1021 gaccgttctc ggtgttttca aagcggccat cggcaaaata tcctttggtt ttcgctttac
     1081 gctgatacgt aatcagacaa tcaggaagtt gactgccgaa cagttcccgg ttgaaaaagt
     1141 cataggctgt ttgaaattcg gtataggttt cgctggttgg ttttttatcc atattctgtt
     1201 tatccttatt tggatcgtac gaaacatatt atatcatcat ttggtttata aagtctttgc
     1261 gaatatttat tggggaatga aaaaaacgac ctaaatgatc gtttttttgt tatacccgga
     1321 ctatcatgat tgattagcga ctactgtttc aatttcgcca gcgcctcggc gagggcggta
     1381 ttgataggag catcatccga ctggttatgc aaatacttct ggacctcccg tttgttagcg
     1441 ccggcttttt ccgtttcacg ccgcttggta aatgcggaga gcttctcacg ataaccgcag
     1501 ctgcaggtga atattttgcc ctccccctca ccgcgtagct ccatcttttt atggcattgc
     1561 gggcaacggg cattggaatg aaccgagatg ctttcccggt agccgcagga tcgatcctga
     1621 cataccagca tttctccgtg tttccccttg acctgtaaaa gatacttccc gcaatcggga
     1681 cacttggcgc gggttaagtt atcgtgacgg tatgtttggc tgcttccggc cacggcaccg
     1741 accaactgaa ccgtataatt ccggatctct ttgacaaaat cggtatgatc ggcccggcct
     1801 ttgccgatct cgcttaattg ccgctcccat ttggccgtta attccggtga tttgagctcc
     1861 ggggggacca ggccgatgag ttgaacccct ttggaagtgg ggatgatctc tttaccgtga
     1921 cgttccatat aaaaagagtt gaatagtttc tcaatgatct ccgcccgggt tgcgggtgtg
     1981 ccgaggccgc tgctattctc cagcgcctcc cggagcccgt gatcttgaat attcttaccc
     2041 gggtgctcca tggcagtgag tagtgtggcc tcggtatacc gggcaggcgg tttcgttttt
     2101 ccggctgcca tatgaacgga ttgaattttg ataacctcgc cctcatgaaa ctccggaagg
     2161 gtttgcgccg tttcccggcc ggtcgggtct ccggaatcat cgaaatcggc ttcttcctcc
     2221 aggttgccga gattttggta gattcttcgc catccctgca acttaacggt ttttcctccg
     2281 gccgtaaaga cctctccgtt cacacccacc tgtaatgtgg tttcctggta ctcaaaagcc
     2341 ggcgaaagca cggccaggaa ccgtttgacg atcagatcgt agattcggaa ttcttcggag
     2401 ttgagtttac tcaagtaaac cggctcttca gtggggataa tggcatgatg atccgtgacc
     2461 ttggcattat caaccaatct tttggtgggt acaatctttg acttcaagat ggcctgaacc
     2521 gattcggcat aaggtcctgc atcagccaca ctgcggagtc gttccggcag cgtcggtacg
     2581 atatcctcgc tgaggtagcg cgaatccgtt ctggggtagg tcaccagctt atggctctca
     2641 ta
//
LOCUS       DASVMB010000228         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_617120,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000228 DASVMB010000000
VERSION     DASVMB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617120"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..799
                     /locus_tag="VF531_06700"
     CDS             167..799
                     /locus_tag="VF531_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX7713694.1"
                     /translation="MPIPFGIWNYLNLLVLVILNLIVLGYLIGRLLKLGNNIAGALRG
                     AWILIVGCKIFDQVYRIPITPPWIGLLKKSWWLTMGLTAAFWVCFPGVALSILMGGLA
                     LLHILWYWLSLRERPENFNQMIHLNTNWGIVFQEIESCSNLNRRSLAELDLRKKNLLV
                     LAVERKGQFFPFPKGLETMTVGDRVYIFGDLEAYHAITTKEAPAVDSNEV"
     gene            946..2700
                     /locus_tag="VF531_06705"
     CDS             946..2700
                     /locus_tag="VF531_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7713695.1"
                     /translation="MGKRDQLSQHFRGKISFQTFLCLVQAGILVVGIFITGIFFYYQA
                     ATRLDREMGRSIISVARTGVSLLNGDQLAALQTGDDDTAYYHQAFQVLQRLQKNSGVK
                     YVYSMRLADDGKAHYVVDADASQDHCSINQEYGEPETEMRRAFQGFITVNPKPVWDKT
                     ERIWSRSAYAPVRNSSNQVVGIVGVDIGADQIQNDKRNLIIALMIVCLFAAGVATLVA
                     FLISSRIMNPLQGLIRRLNQIAASQGDLSKRLEISTMFKEIAMLAETVNRVQDNSRNI
                     VKAVSRLTGSLNTISQQLVHSVENASATSEEVAAMMNQIQLGAEDQSNSTGSIAELME
                     EMTSGASEVASSAAKAEEVVLRSSEAAVGGEASIQETLKLLHQVVDFAVRLKDFTSAL
                     SRRSEEIRKILSLLSYMSRQTKILSLNANIEASKAGDSGQGFAVVAREIRTLADNSQT
                     SLGEITSEVTELRNELGQIVKMANEFHALLNTSDSMIKQAEMKLGEIKERSAESFTFT
                     SQISRLVQDQVRETDQTNSFVQKITEISKQFAISSETVVTAVSEQAQTLQGILDVSQR
                     LQDIVADLHKMVGECKLD"
     gene            complement(2713..3126)
                     /locus_tag="VF531_06710"
     CDS             complement(2713..3126)
                     /locus_tag="VF531_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7713696.1"
                     /translation="MKHELEEGPLLQLDFSKIGQIAAKCSDVIPVAVQNADTKEVILV
                     AYVNETALQTAISTRTAVFWSTSRNELWVKGQTSGETFELLEVYVNCEQNSLVYMVRP
                     RKGGICHTKNLNGEPRNCYYRRLNFTTGKLENINP"
BASE COUNT          900 a          729 c          840 g          934 t
ORIGIN      
        1 aatcaagcaa cgaatgcttg ttttttttgt aattattttt gctggaagtg taggcattta
       61 tcggaacatg atgtcatatc ctttattaac ccctccagag tcgattcagg cggtctttta
      121 tttttgaaga tttgccggga aaatgtcgtt tcaaagggtg agaatcatgc ctattccttt
      181 cggcatatgg aattacttga atctacttgt cctggtcatt ctaaacctga ttgtcctggg
      241 atatctgatt ggtcgcctat tgaagctggg caacaatatt gcaggagcgc tccgtggtgc
      301 ctggatactt atcgttggct gcaaaatttt tgatcaagtt taccgtattc ctatcacgcc
      361 gccatggatc gggttattaa aaaaatcctg gtggcttacg atgggattaa ccgctgcatt
      421 ctgggtttgt ttccccggcg tagccttgag catcctcatg ggggggctcg ccttgcttca
      481 tatactgtgg tactggctat cgcttcggga gcgtccggaa aacttcaatc agatgatcca
      541 tctcaatacc aactggggta ttgtatttca ggagattgag tcctgttcca acttaaaccg
      601 gcgttcattg gcggaactcg atttacgtaa gaaaaatttg ctggttttgg cggtggaacg
      661 aaaaggacag ttttttccct ttcccaaggg acttgagacc atgacagtcg gagatcgggt
      721 ctacattttc ggggatttgg aagcttatca cgcaatcact accaaggagg cacccgctgt
      781 cgattcaaat gaagtttgag atttaaacgt ccgcaagata ttgacaaccc gattcaaaaa
      841 tttggatcgg gctttttttg catttttaat tcaaaaacgg tagaaaaatg acgatgacat
      901 cctatgcttg ccttgattac agcttagatg aaggggaaaa ctcctatggg gaaaagagat
      961 cagttatcac agcattttag gggaaagata tcgtttcaaa cgtttttgtg tttggttcaa
     1021 gccggaattc tcgtggtggg aatctttatt accggaatat tcttctacta tcaggctgct
     1081 acccggctag acagggagat gggccggagt attatttcag tagcccggac aggggtatca
     1141 ttattaaatg gagatcaatt ggcggcgtta caaaccggtg atgatgatac agcctattat
     1201 catcaggcat tccaagtatt acagcggctt caaaaaaatt ccggggttaa atatgtatac
     1261 tccatgcgat tggccgatga cgggaaggca cattatgtcg tggatgccga tgcttctcag
     1321 gaccattgtt caatcaatca agagtacggg gaaccggaaa ccgaaatgcg cagggcattt
     1381 caaggattta taacggttaa ccccaaaccg gtgtgggaca aaaccgaacg gatatggagc
     1441 cgttccgctt atgctcctgt aagaaactca tccaaccaag tggtgggaat tgttggagtc
     1501 gatatcgggg cggatcagat tcagaatgac aagcggaatt tgatcattgc cttaatgatc
     1561 gtctgtttat tcgctgcagg agtggccact ttggtcgcat ttctcatctc ctcccggatc
     1621 atgaatcccc tccaaggctt aatcagacgt ttaaatcaaa ttgccgcgtc tcagggcgat
     1681 ctttcgaaac gcttggagat ttcgacgatg tttaaggaaa ttgcgatgtt ggccgaaacg
     1741 gtgaaccggg ttcaggataa ttcgaggaat attgtgaagg ccgtatcccg attgaccggt
     1801 tcattgaaca ccatcagtca acaactggtt cattccgtcg aaaacgccag tgctacatcg
     1861 gaagaggttg cggcaatgat gaatcagatt caattaggtg cagaggacca atccaacagt
     1921 accggtagca tcgccgagtt gatggaggaa atgaccagcg gtgcttccga ggttgcgagt
     1981 tcggcggcga aagccgaaga ggtggtactt cgttcttccg aagccgcggt tggcggggaa
     2041 gcatccatcc aggaaacgtt gaagctcctc catcaagtgg ttgattttgc cgtgcggttg
     2101 aaagatttta cttcagcgct gagccgacgt tctgaagaaa tccgtaaaat cctttctttg
     2161 ctttcctata tgtcacgcca aactaaaatt ttgtcgctaa atgccaatat tgaggcctcc
     2221 aaagcaggag attccggtca gggattcgca gtggtcgccc gggagataag aactttggcc
     2281 gataattctc agacgtccct gggggaaatc acttccgaag ttacggaatt acggaatgaa
     2341 ctcggccaga ttgtgaagat ggccaatgaa tttcatgccc tgttgaacac cagcgacagc
     2401 atgattaagc aggctgaaat gaaactcgga gagatcaagg aacgttccgc agaaagtttc
     2461 acctttacca gtcagatttc gcggttggtc caagatcagg ttcgggaaac ggatcaaacg
     2521 aattcgttcg tacagaaaat taccgaaatc agcaaacaat ttgcgatatc cagtgaaacg
     2581 gtggttacag cggtatctga acaagctcaa acactccaag gtatcctgga tgtttcccaa
     2641 cggctccagg atatcgtggc ggatctccat aaaatggtcg gggagtgtaa gttggattaa
     2701 agaacattca actcacgggt tgatgttttc cagctttccg gtagtaaagt tcaggcggcg
     2761 gtagtagcag ttccggggct cgccgtttaa gttcttggta tggcaaatac cgcctttcct
     2821 tggccgaacc atataaacta aggagttttg ttcacagttc acataaactt ccaataattc
     2881 aaaggtctcg ccgctcgtct ggcctttcac ccaaagctca ttgcgggatg tagaccaaaa
     2941 aaccgcggtt cgggtggaga tagctgtctg taaagcggtt tcgttcacat aagccaccag
     3001 gatgacttct ttggtatccg cgttttgaac cgctaccggg attacatcgg aacatttggc
     3061 cgcgatctgg ccgatctttg aaaagtccag ctgtaaaagg ggtccttctt caagctcgtg
     3121 tttcatggac aacctcctca atgaatgagt actcggcaat gaaagcccat ccggtcgaaa
     3181 tcattgctat cgtgcttatt cgtaggacga atgcttggat atactatacc acacaacccc
     3241 tggccatgac cagaaaaatt tgggaaaatg atcggcgcga tctcccggtc atattttgtc
     3301 tttctttttg atatggcttg cggtaagata ggataggtaa atggattgtt ttccaatcat
     3361 aattgccaaa agcaagtgtg aaaaccggca tccttgtatt cat
//
LOCUS       DASVMB010000229         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_28062,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000229 DASVMB010000000
VERSION     DASVMB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28062"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VF531_06715"
     CDS             <1..635
                     /locus_tag="VF531_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7713697.1"
                     /translation="ERALRCADHEAIHDFLNHVFEAVIPISLGPEEARWMVLDFVNTL
                     RRILVEYQVHWEEIAHGESTFPEILEQAESIRDWQIGIEKLVDCYLQLVKASVYPQVS
                     LTIRKALMFIQSNFTRDLSLEEVACHAGVSKSYLCRVFPEYAGEHFSDYLQRLRVERA
                     KELLLFTNDHIYEIATKVGFWNSRYFSKVFHDMVGMTPADYRRIPSKTKA"
     gene            945..2216
                     /locus_tag="VF531_06720"
     CDS             945..2216
                     /locus_tag="VF531_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713698.1"
                     /translation="MNIQYSQIRFISRTIFIVLLFLTTILPASAKEMGVRITIWTINQ
                     PVLDLTGDWMELEIKKYEAANPGITIDHRFWENQSYKTKLKVAMFGGEGPDILFNWGG
                     ESQLIYSREGLLYDLTRDLGKDKWGLSPGMFATHSYQGRIYGIPLFPSVEVVWYNKAI
                     FRKNHWRTPQTWEEFLALCDKMKTKGYVPIAMGGQEPWTILYPYMYLVDRLAGNELYF
                     AAKSRQKRFTYPDFVQAFRLLQQLALREYLPAEVLSTSYIEATQLMIHNKALMMFMGD
                     WEYQQLTLQMRQDLEKWDFFPFPVLKGGKGSAQNIVGAVAGFSIKKSENSGEALKFLK
                     FLSSKESLVDSYRMTGKLVTLATPYMGKNDRPQIKGIAQLLTNASSLMQWWDQDLPEP
                     VTQCLLQSLQELLAGRCSPEQAAALIETAYD"
     gene            2511..>3387
                     /locus_tag="VF531_06725"
     CDS             2511..>3387
                     /locus_tag="VF531_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713699.1"
                     /translation="MKKTLHLIGVLLLIALLAGVSIVCAAEPVKLTMWSIQRPNETDS
                     LGLCMMNQIKAFEKANPDIQIEYNYTENEAYKTKLQVAMMGGAGPDIFFNWGGETQAI
                     YSREGMLYDLTKAVAKDYWGLSKALFSNHTYNGKIYGIPFFPTVNTVWYNKKMFAQYG
                     LTPPKTWSELLTVCEELKKQGIIPFTVGGKDSWTILHAFMYIADRTAGAKLYQNAKAG
                     IARFDDPAFVKAFGMLETLVKKGYFPPDALNLTYGDASQLMMKDKAAMMAMGNWHFTM
                     ITNENKSEFDKWDCFT"
BASE COUNT          910 a          746 c          849 g          882 t
ORIGIN      
        1 gggagcgggc gctgcgttgt gcggatcatg aagcgatcca tgatttttta aatcatgtct
       61 tcgaagcggt aattccaatt agcctgggcc ctgaagaagc ccgctggatg gttctggatt
      121 ttgtcaatac cttgcggcgg atcctggttg aatatcaagt acactgggaa gaaattgccc
      181 atggagaatc aacttttccc gaaatcctgg aacaggccga atcaattcgg gattggcaga
      241 taggcatcga aaaactggtt gactgttatc tgcaattggt caaagcaagt gtttatcccc
      301 aagtctcctt gaccatccgg aaagcgctga tgtttattca gtccaatttc acccgggatc
      361 tctctttgga agaggtcgcc tgtcacgccg gggtcagtaa aagttatctt tgccgggttt
      421 ttcccgaata tgccggggaa cacttcagcg actacctgca gcggctgcgg gtggaacgcg
      481 ccaaagaatt actgttgttt accaatgatc atatttatga gatagctaca aaagtcggtt
      541 tctggaatag ccgttatttc agtaaagttt ttcatgatat ggtggggatg acccccgccg
      601 attaccgccg gattccttcg aagacgaagg cgtgaggtgt gaggcatgag gcatgaggca
      661 agaggcaaca ggcaacaggc aacaggcaac aggcaacagg caataggcaa taggcgtaag
      721 gcatgaggca actcacccca cgttaccggc aagttttcat tacaacccta ctcccctctc
      781 tttcggaaaa gagaggggtc gggggtgagt tgctaatagg catttgctta ttgagaaggc
      841 aaaaggcatg ggactggacg caccgggcag catgagtttt ggcttgattt atcttttcat
      901 attaagggta cttccggggt agccccaagg gatggaggaa cgggatgaac attcaatatt
      961 ctcaaatccg atttatttcg agaaccatat tcatcgttct gttattcttg acaacaatcc
     1021 ttcccgcctc agccaaggag atgggggtcc gtattaccat atggacaatc aatcaaccgg
     1081 tattggactt aaccggtgat tggatggagc ttgagattaa aaaatatgaa gcagccaatc
     1141 ccggcattac aattgaccat cgattctggg agaaccagtc ttataagacc aaactgaagg
     1201 tggccatgtt tgggggtgaa gggccggata tcctttttaa ctggggcggc gagagccaac
     1261 ttatttactc cagggaaggt ctattatacg atttgacccg ggacttgggg aaggataaat
     1321 ggggtttatc cccggggatg ttcgccactc atagctatca ggggaggatt tacggcatcc
     1381 ccctgtttcc ttcggttgaa gtggtttggt ataataaggc aatttttcgg aaaaatcatt
     1441 ggcgcacccc ccaaacctgg gaagaatttc tggccctttg cgataagatg aaaaccaaag
     1501 gatatgttcc catcgccatg ggcgggcagg agccttggac gattctttac ccctatatgt
     1561 acctggtgga ccgcttggcc ggaaatgaac tctattttgc ggccaaaagc cgtcaaaagc
     1621 gtttcactta tcccgatttt gtgcaagcct tccggctcct gcaacagctg gcgttgcggg
     1681 aatacttgcc tgcggaagtt ttaagcacca gttatatcga agccacccaa ttgatgattc
     1741 acaataaagc cttgatgatg tttatgggtg attgggagta tcaacagctt acgctgcaga
     1801 tgcgtcaaga ccttgaaaaa tgggactttt ttcccttccc cgtgttgaag ggtggaaaag
     1861 gatcagctca gaatattgtc ggggctgtgg ccgggttttc gattaagaag tcggaaaatt
     1921 ccggggaggc ccttaagttt ctcaagtttc tctccagtaa ggaaagtttg gtcgatagtt
     1981 accgcatgac cggcaagctg gttaccttgg caacgccata tatgggtaaa aacgaccggc
     2041 ctcagattaa aggaatcgcc caattgctga ccaacgcctc ttcactgatg caatggtggg
     2101 atcaggacct gccggaaccg gttacccaat gtctgctcca gagtttgcaa gaattattag
     2161 ccgggcggtg tagccctgaa caggctgcgg cattgattga aacggcatat gattaaaaaa
     2221 tgaatggtca atattttggc atcaaaggac aatgttttat cactagtcca atgttcatca
     2281 ttttgttaca atttatttgg agaagacgtt ccggaacgtt cccatcccta tctagaaaga
     2341 acctaaggcc tttttctaga taggtcaacc agctcgatga tttcccttca cttcgttgtg
     2401 gggcagaact gcaatggtta caccgggttg gctgacccta gaattatgaa gctgcttatt
     2461 ctcttaagca ttcatagttc actgaaaaag agtaggaagg agatttcgga atgaaaaaaa
     2521 cattgcatct cattggagtc ttattgttga tagcactgct cgcgggtgtg agcatcgtat
     2581 gcgccgctga gccggtgaaa cttaccatgt ggagtattca acggcccaat gagaccgatt
     2641 cgcttgggtt atgtatgatg aaccagatta aagcctttga aaaagccaat cccgatattc
     2701 aaatcgaata caactatacc gaaaatgaag cttataagac caaattgcaa gtggccatga
     2761 tgggcggcgc cggtccggat atctttttta actggggcgg agaaacgcag gccatctatt
     2821 cccgggaggg aatgctctat gacctgacca aagcggtcgc caaagactac tggggtctat
     2881 caaaagcatt attctccaac catacttata atgggaaaat atacggcatt ccctttttcc
     2941 ccacagtcaa caccgtctgg tacaacaaga aaatgtttgc ccaatacggc ttgactcccc
     3001 ccaaaacctg gtcggaatta ttaaccgtct gtgaagaact gaaaaagcag ggtattattc
     3061 cgtttaccgt tggcggtaaa gattcttgga cgattttgca tgcttttatg tatatagctg
     3121 accggactgc cggagcgaaa ctgtatcaaa acgccaaagc cggtatagcc cgttttgacg
     3181 atccggcttt tgttaaagcc ttcggcatgc tggaaaccct ggttaagaaa gggtatttcc
     3241 ctccggatgc tttaaacctc acctacggcg acgcttccca attgatgatg aaagacaaag
     3301 cggccatgat ggccatggga aattggcact ttacgatgat aaccaatgaa aacaagtctg
     3361 agtttgataa atgggattgc ttcacct
//
LOCUS       DASVMB010000230         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_28218,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000230 DASVMB010000000
VERSION     DASVMB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28218"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VF531_06730"
     CDS             <1..993
                     /locus_tag="VF531_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713700.1"
                     /translation="IFQSWGGSTMGGYADMGQLLDLTKELKSIPGSAAAQQAMTWKDK
                     IYGVAPFFAIAGVFYNEGMFKKYNLKVPTTIDQLEKVADTLKSKGVQPFACGARDKWP
                     VLALFMYLTDRSGGVQVFNDASARKSRFDSAEFVKAAQKYQEWAKKGYFGDKPLGEAY
                     NDSQQLVATGKAAMHITGSWMCAQYSNKEFTDQTIGFYAFPEMKGGKGKITDVMGMPD
                     IAFAATKAAANKKDAVVRFMKYAMSVEACQAEPGRICSVPGVKAPSRLTGMAAGVFGK
                     AKAVQFWWDQNLPPMVTTPLLDAMQNIFLPDGDVKKAFATYESLVEENMGPVKK"
     gene            1136..2071
                     /locus_tag="VF531_06735"
     CDS             1136..2071
                     /locus_tag="VF531_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713701.1"
                     /translation="MQESMSVSGSPSNENRQKKQDLNAIFFKTFFLLPAVVFLVVFMY
                     YPIEQTFHLSLTRTTGLGQPVFIGFENYLRLFTNEEFQAGLIHVFGWAFWSVVIQIPL
                     TFFIAFSLSAYRNKITNPLRAVYYLASILPSAIVAMLGRFVFAPNFGIIASLSAKLNW
                     GWLAKIDFLGDPQLAFWSVFAVATWAYSGFYIVYLMANIEQIPVEIREASQLDGANRW
                     QYARHIVLPMVANPLRIIGILCVVGSLKIFDLPYLLTTGGPGYATTTLGIIIYQQGFR
                     NWQYGKAAAVGVVIFLLSLIFTVAQFSWQRKEGDM"
     gene            2071..2946
                     /locus_tag="VF531_06740"
     CDS             2071..2946
                     /locus_tag="VF531_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713702.1"
                     /translation="MNHASIDTNREDQYVRTRPTIAGVLITTVLILLALTVILPLLMP
                     WLFVFKTRLECSYNPWSLPTQIRWVNFIEAWVAVQIDQGLLNTVIVCLGAVLCTVPPA
                     AMAGYIFARYRSKVTEVVFYCILAGYFVPVQMILIPLYKLCSQMNLTDTLPGLFLPLA
                     AFGIPFWTIIYRSFFQGLPGELAEAARIDGAGHTGTFFRIMLPLAKPATVLAVMLVFI
                     AAWSDYLLSLILINSQKLFTMQLRIAQFLNAYGVDNMPRYAAAAIISAAPTILLYILG
                     HRWILKGTLAGALKG"
     gene            complement(3104..>3759)
                     /locus_tag="VF531_06745"
     CDS             complement(3104..>3759)
                     /locus_tag="VF531_06745"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713703.1"
                     /translation="LTGGVIRMAGRKVERPDKDRMVVFQSFDQLFPWLTVLQNITFAL
                     QATRTVIDSKARAATARSYLQLVGLSGYADYFPHQISGGMKQRVAIARALSVRPRILL
                     MDEPFGSLDALNRNLLQEELIRIWQNTGVTIIFVTHSIEEAILLSEKILVLETGPGRI
                     RALLTNSIPRPRSPEAPEFTTLWKELHGLLGLYRETGTAVEPQRRIVFEDGILQPSR"
BASE COUNT          872 a          892 c          973 g         1022 t
ORIGIN      
        1 attttccaga gctggggcgg ttccacgatg ggcggatacg ccgatatggg ccagctgctc
       61 gatctgacca aagagcttaa atccatcccc ggtagcgccg ccgctcaaca agcgatgacc
      121 tggaaagaca agatctatgg agtggccccg ttctttgcca tcgccggtgt attttacaat
      181 gaaggcatgt tcaaaaaata taatctgaaa gtgccgacta caatcgacca attggaaaaa
      241 gtagccgata cgttaaagag taaaggtgtt cagccttttg cctgcggtgc cagagataaa
      301 tggccggttc tggctttgtt tatgtaccta accgaccgaa gcggcggcgt tcaggttttc
      361 aatgacgcca gtgcccggaa gagtcgtttc gacagtgctg aatttgtcaa agccgctcag
      421 aagtatcagg aatgggcaaa gaagggttac ttcggcgata agccgctggg tgaggcgtat
      481 aacgattctc aacaacttgt ggctacgggt aaagccgcaa tgcatattac tggttcctgg
      541 atgtgcgccc aatattctaa caaggaattt accgatcaaa ctattggttt ctacgctttt
      601 ccggaaatga aaggcggcaa gggcaagatt accgatgtga tgggcatgcc cgatatcgcc
      661 ttcgctgcta ccaaagctgc cgccaataag aaggatgcgg tcgtgcgctt catgaagtac
      721 gcgatgagtg tggaagcctg tcaagccgaa cccggacgta tctgttccgt gccgggcgtg
      781 aaagccccct cccgtttgac cgggatggct gccggtgtat tcggtaaggc taaagcggtt
      841 caattctggt gggaccagaa tttaccgccg atggtgacca ctccgttgct cgatgcgatg
      901 cagaatattt tcctccccga tggcgatgtt aaaaaagcct ttgccacata tgaatcattg
      961 gtggaagaaa atatgggacc ggttaaaaag taagcaatcc gttccgtgac aattgttggc
     1021 ggaggcgtgg attgtttctg cctccgccgg cttttgtttg agaggaaagg atgaacccag
     1081 aggttattcc tcaccccgga aacatgataa tttgaaagac attgaggtgt tgggaatgca
     1141 agagagcatg agtgtgagtg gttctccgtc taatgagaat cgtcaaaaaa agcaggactt
     1201 aaacgctatt tttttcaaaa cttttttcct tttgcctgcc gtagtgttcc ttgttgtttt
     1261 tatgtattat ccgattgaac aaactttcca tctctccctc acgcgtacca ccggccttgg
     1321 tcaaccggtt ttcatcggat ttgagaatta tctgaggcta tttaccaacg aagagttcca
     1381 ggcgggtttg attcatgttt ttggctgggc attctggagc gtggtcattc agatcccctt
     1441 gacgttcttt atcgcctttt ccttatccgc ttacaggaat aagattacca atccgttacg
     1501 cgccgtttat tatctggcgt cgattttgcc gtcggcgatt gtggcgatgt tgggacggtt
     1561 tgtttttgcg ccgaacttcg gtataatcgc ctctctctcg gccaaattga actggggttg
     1621 gctcgcgaaa atagactttc tgggagatcc gcagctcgct ttctggtcgg tatttgccgt
     1681 tgccacctgg gcttactccg ggttctacat tgtctatctc atggccaaca tcgaacagat
     1741 accggtggag attcgcgaag cgtcgcagtt ggatggcgca aaccgctggc aatatgcaag
     1801 acatatcgta ctgccgatgg ttgccaatcc gctccgcatt attggtattt tatgcgtcgt
     1861 cggcagctta aagattttcg atttacccta tcttctcacc actggcgggc cgggttatgc
     1921 tacgacaaca ttgggtatta tcatttacca acaaggtttc agaaattggc aatatggtaa
     1981 agcggctgca gtaggtgtgg tcatattcct gctctcgtta atctttaccg tggcccaatt
     2041 ttcctggcaa cgaaaagaag gtgacatgta atgaatcacg catcgattga tacaaaccgg
     2101 gaggatcaat acgtcagaac ccgtccgacc attgccggag tgctaattac tactgttttg
     2161 attttgctgg ctttaacggt tatcttaccg cttcttatgc catggctctt cgtattcaag
     2221 acccgtttgg aatgttccta caatccatgg agcctgccta ctcagatacg ttgggttaat
     2281 ttcattgaag cctgggtagc cgtacagatc gatcaaggtt tacttaatac tgtgattgtc
     2341 tgtttaggcg cagttctctg tacggtgccg cctgcggcaa tggcgggtta tatttttgcc
     2401 cgttaccgga gcaaagttac ggaagtggtg ttttactgta tcttagcggg ttatttcgta
     2461 ccggtgcaga tgatcttaat tccgctttat aagctatgtt cacaaatgaa cctcaccgat
     2521 acgttgcccg gactgttttt gccgctggcc gcattcggta ttccattttg gacaatcatc
     2581 tatcgttcct tctttcaggg cctgcccgga gagctggcgg aagcggctag aatcgatggc
     2641 gccgggcaca cgggtacttt tttccggatc atgttgccgc ttgccaagcc ggcgacagtt
     2701 cttgccgtca tgctggtctt tatagccgct tggagcgatt atctattgag cttgatcctg
     2761 attaacagtc aaaaactctt taccatgcag ttgcggatag cgcaatttct taacgcctat
     2821 ggtgtggaca atatgccgcg ctatgctgct gccgcaatta tctcggctgc gccaacgatc
     2881 ctgctatata tcttaggtca tcgctggatt ctcaagggaa cactggccgg agccttgaag
     2941 ggataaatga attgccaagg tatagatccc tcgctatttt tttgaaatag atggccgggc
     3001 atattatgta gggacccggg gagtttctga aggccccaaa tacttcggag acaatgggat
     3061 cgggctttga gagcctgatc tgaaactatc atattaagag tattcaacga gacggttgca
     3121 ggatgccgtc ctcaaagacg atccgtcgtt ggggctccac cgccgttccc gtttcacggt
     3181 agagtcccag caacccatgg agttctttcc aaagcgtggt aaattccggt gcttccggtg
     3241 agcgtggtct gggaatggag ttggtgagca gggctctgat ccgccccggc ccggtttcca
     3301 agactaaaat cttctcgctg agtaggatag cctcttcaat gctgtgagtc acgaagataa
     3361 tggttacacc ggtattttgc cagatccgga tgagttcctc ctggagtaga ttgcggttga
     3421 gcgcgtcaag actaccgaaa ggttcatcca tcaatagaat tcggggtctg accgagagag
     3481 cccgggcgat ggcaacccgt tgtttcatac cgccggaaat ctggtgcggg aaataatccg
     3541 cataacccga gagccccacc aattgcaaat aactacgggc agtggcggcc ctggccttcg
     3601 aatcaatcac cgtccgggta gcctgtaagg caaaggtaat gttctggagc acggtcaacc
     3661 agggaaagag ttgatcgaaa ctttgaaata ccaccatccg gtccttatcc ggccgttcaa
     3721 cttttcgacc ggccatgcgg ataacgccgc cggtcaacg
//
LOCUS       DASVMB010000231         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_691992,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000231 DASVMB010000000
VERSION     DASVMB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691992"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VF531_06750"
     CDS             complement(<1..1458)
                     /locus_tag="VF531_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7713704.1"
                     /translation="MGKIEEFDVAIIGAGVIGAALARELSRYQLSIALLEKENDVGCG
                     TSKANSGIVHSGIHEDPGSTKGRLCIAGNRLYPALAGELDVLYQNNGSILVAKSEAEF
                     PVLESMYQNGIANGLTGIESLNRRQLLDLEPHLSPELAGGLLVPGAGTVVPFDLVFAL
                     VENALDNGVQLRLGTEVVKIETQTGHFIIQTRQRGVLRARYMVNAAGLYGAMIAGLIG
                     DHSFTIAPRKGEEYLLDRRLEGLVRRTVFPLPDKTSKGILIIPTVDGNIMIGPTAKST
                     DKLTDDSTSSSGWQEIFENAQSLVPSLRPADLITSFAGIRAVSPDEDFILRPSPLNPR
                     FIHAAGIASPGLTAAPAIALELVELLREAGLILKSKQRFNPERKLIRLRQLNREEQAE
                     LMERDRRYANIVCRCELVSEAEIRTAIRRGATTVDGVKLRTRAGMGRCQGGFCTPRII
                     QILSEELGIDPTAVRKKGPCSELLTGWLREKAGVPG"
     gene            complement(1644..>2993)
                     /gene="glpK"
                     /locus_tag="VF531_06755"
     CDS             complement(1644..>2993)
                     /gene="glpK"
                     /locus_tag="VF531_06755"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX7713705.1"
                     /translation="LIWKTVHDGLSEILASPILAGGRVKAIGITNQRETVVVWDRTTG
                     KPIHPAIVWQCRRTAERCQELIRKGYGRKISVKTGLRIDPYFSATKIEWILNQVPGAR
                     DKAARGELACGTIDSWLIWKLTGGRVHATDPSNASRTMLFNIHELRWDLELLRLFGIP
                     PAMLPEVKPSASFFGETASSACAGDPIPIAGVLGDQQAALFGQGCFDPGAMKNTYGTG
                     CFLLMNTGPKAYQPQNGLLTSIAWSLNGKVDYVLEGSVFMGGAVIQWLRDELGILTAA
                     SDSERMALEVPDTNGVYLVPAFTGLGAPHWDPYARGLIIGLTRGVNRSHLVRAALEAI
                     AYQVREVIESMCRTVKIRPQSLRVDGGAAANNFLLQFQADICNLQIERNNSLELTGIG
                     AAFIAGLTAGFWENTDSLKGLIQLERAFTPGMNATDRQRLCNGWNKAVDRAKSWDKD"
BASE COUNT          674 a          845 c          773 g          701 t
ORIGIN      
        1 tcctggcacc ccggcctttt cccgcaacca gccggtgaga agctcgctgc agggtccctt
       61 cttacggacc gccgtaggat caatccctaa ttcctcactc aagatttgga taatccgggg
      121 agtacaaaat cctccctggc agcgccccat tcctgctctg gtccttaatt tgactccatc
      181 gacggtggtg gcacccctgc ggattgccgt acggatttcg gcttcactca ccaattcgca
      241 gcggcaaacg atgttggcgt accggcgatc ccgctccatc aattctgcct gttcttcacg
      301 attgagctga cgcaagcgga ttagtttccg ctctgggtta aacctttgtt tggatttgag
      361 tatgagtccg gcttcacgga gtagttccac cagttccagc gcaatggcgg gggcggccgt
      421 cagccccggc gaagcgatcc cggcggcgtg aatgaaccgg ggattgaggg gagaaggccg
      481 gaggataaag tcttcatccg ggctgactgc ccttataccg gcaaatgatg tgatcaaatc
      541 ggcaggccga agcgatggga ccaatgattg cgcattttca aaaatctcct gccaaccgga
      601 actgctggtg gaatcatcgg ttagtttatc cgtacttttg gcggttgggc cgatcatgat
      661 gttcccgtcc accgtgggaa tgatcagaat acctttcgat gttttgtcgg gaagggggaa
      721 gacggtgcgc cgcaccaaac cttccaatcg ccgatcgagc aagtattctt cgccctttcg
      781 gggggcaatg gtgaaggagt ggtccccgat caaacctgca atcatcgctc catacaaacc
      841 ggccgcattg accatgtatc gggcgcgtaa tacgcctcgc tgacgggttt ggataataaa
      901 atgaccggtt tgggtttcga tcttgacaac ctctgtgccc aaccgtaatt gaacgccatt
      961 gtccagtgca ttctccacga gggcgaagac caggtcaaaa ggtaccacgg ttcccgcccc
     1021 ggggaccaac aatccccccg caagctccgg ggagagatgg ggctccagat ccagcaactg
     1081 ccggcgattt agggactcaa tcccggtcaa accgtttgct attccgtttt gatacatcga
     1141 ttccagcacg ggaaactcgg cctcactttt ggccacgagg atcgaaccgt tattttgata
     1201 tagaacatcc agttccccgg caagtgcagg gtaaagccgg ttcccggcga tacaaagacg
     1261 gcctttggta ctccccggat cttcgtgaat tccggagtgg acaatgccgc tgttggcttt
     1321 ggaagttccg caaccgacat cgttttcttt ctcaagtaac gcaatggaga gctgatatcg
     1381 cgagagttcc ctggcaagtg cggcgccgat gacgcccgct cctattattg ctacgtcgaa
     1441 ctcctcgatt ttacccaagc taaaacctcc tttgaaggaa aataaaaaag gccaaaaaat
     1501 ttagggacac ggcaggtgtc ctaaacttct tggccctctc atcatctctg ggcgtatgaa
     1561 caattacttt aatttaagtg taagccggtt ttgggtattt gtcaatccgg atcttcaaat
     1621 tataaacagg tatagctgtt tcatcaatct ttatcccaac ttttggcgcg atccaccgcc
     1681 ttgttccagc cgttacaaag cctttgccga tccgtggcgt tcatcccggg tgtaaaggcc
     1741 cgttccaatt ggattaatcc tttcagtgaa tcggtattct cccagaatcc cgcggtcaat
     1801 ccggcgatga aggccgcacc gatccctgtt aactccagag agttattgcg ctctatctgc
     1861 agattgcaga tatcggcctg aaattggagt aaaaagttat tcgccgccgc gccgccgtca
     1921 acccgtagac tttgaggccg gatcttaacg gtccggcaca tgctttcgat aacctccctc
     1981 acttggtagg caatggcctc caaagcggca cgtacaagat gagaacggtt gacccccctg
     2041 gttaatccga taatcaaccc tctggcataa ggatcccagt gcggcgctcc cagtccggta
     2101 aaagccggaa ccagatagac tccgttggta tccggaacct caagtgccat ccgctcggag
     2161 tcggaagcag ccgtcaagat ccccagttca tcacggagcc actggattac cgccccgccc
     2221 atgaagacgc tgccttccag gacgtaatcc accttgccgt ttaaactcca ggcaatactg
     2281 gtgagtaagc cgttttgggg ttgataggcc ttgggaccgg tgttcatcaa gagaaaacaa
     2341 ccggtaccgt atgtattctt catcgcgccc ggatcgaaac aaccttggcc gaagagcgcg
     2401 gcttgctggt cgcccaaaac cccggcaatc ggaatcggat ctccggcaca ggccgatgaa
     2461 gcggtttccc cgaaaaagct cgccgaaggc ttaacctccg gcaacatggc cgggggtatt
     2521 ccgaataatc ttaagagctc gaggtcccac cgcagctcat gaatattaaa gagcatcgtc
     2581 cgggaggcat tggatggatc cgtggcgtga acccgcccgc cggtaagttt ccaaatcaac
     2641 caggagtcaa tggtcccgca ggccaattcg ccgcgggccg ccttgtcgcg tgccccgggg
     2701 acctggttca aaatccactc gattttcgtg gctgagaaat aaggatcaat gcgtaagccg
     2761 gttttgacgc ttatttttct gccgtatccc ttacggatta gttcctggca acgttccgca
     2821 gtcctgcggc attgccagac gatggctggg tggattggct tgccggtggt gcggtcccag
     2881 acaactaccg tttcccgttg gttggtgatt ccaatcgcct ttaccctgcc acccgccaga
     2941 atgggtgaag ccaggatttc agacaaccca tcgtgaacgg tcttccagat taa
//
LOCUS       DASVMB010000232         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_322317,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000232 DASVMB010000000
VERSION     DASVMB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322317"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VF531_06760"
     CDS             complement(<1..497)
                     /locus_tag="VF531_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7713706.1"
                     /translation="MKDGKGVLAYAAICLIWGTGYLANRIGIRSFPPLLFLSLRLCAA
                     GSLLLLYALVKRLPFPRKAGQYLQMFGAGLLLFFGGNGFGILGLQTVTSGAAALIVST
                     VPIFTALIELALPHSSRLGLAGWIGLLIGGCGVAFLLVSGERMQALDLRGCLLVGMGA
                     LCWAAG"
     gene            complement(513..1361)
                     /locus_tag="VF531_06765"
     CDS             complement(513..1361)
                     /locus_tag="VF531_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713707.1"
                     /translation="MAVNDSTFRKKLSRVLFYLLMVVTCVLIAFPIYWLLNSSFQRES
                     ALFSYPPAFFPKLFSVEGFHKLFVDGRIWRWILNTAIVTISTVGLTVIFATLAGYSFS
                     RFRYKGRKTLSLALLSTQMLSGPMVITPLFIVFSKLGLIDSSLSLIIADTGLCIAVNT
                     MLMKGFFDTIPVGIEEAAYIDGCNRMEALFRVTLPLTMTGMVTTLVMTFFVTWNEYLY
                     GLIFISNQDKWIGSVGLASFIGSYVVSQDQILAGASLFSVTPVLFFMFFQKYIVVGVT
                     SGAIKG"
     gene            complement(1381..2262)
                     /locus_tag="VF531_06770"
     CDS             complement(1381..2262)
                     /locus_tag="VF531_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713708.1"
                     /translation="MSVQARRKFDTLPYLLVLPALLVLALVYFYPIIKGLLLSFTDAK
                     FVEQAPFVGLQNYLRMTNDPDFWYSCKITVIYSFFYVVGVFGVGLATALLLNAKFKGR
                     PLARTLLIIPYAIPDIAAVAVWTWLFDYQYGVINYILKGLGLIAQPVQWLANPSVAMF
                     AVLVVSVWRLFPFHSLALLSALQGVPEELYESAELDGATGIKKFFYVTLPSISGIMGI
                     LVLLTVIWSFQRFTIIWALTQGGPARATESLVIQVYLKAFKYYQMGYAGAIGTVMIIF
                     LMLITIGYFILSKEDQK"
     gene            complement(2356..>3268)
                     /locus_tag="VF531_06775"
     CDS             complement(2356..>3268)
                     /locus_tag="VF531_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713709.1"
                     /translation="KYIKPSQYKLESINNEGVIDGHRYALNLMSFAYGQLIYNKKMLA
                     DAGLKPPTTPEELIDVAKKLTKAPDQYGFGMPVVPTETEYFAQGIQKFASGFDAGISK
                     NGKIAVNDPNFVKGVECFKKVYDADVTPKGIGWSVQRRMFADNKIAMIFDGAYQFGII
                     DGFYPGHSNDFGAVRPPFPGNHVLFNENFFGISKASKHKAEAVKFLKWWIRADNQAEY
                     NMFGLHIGTVKTNYPKAWLDQHPYLAAYNDPKLIPMPTMVKGFEEQSEQIRRTLVDNV
                     AQIFFANKPIDQAMNDAEKVLGDIVKK"
BASE COUNT          959 a          824 c          737 g          748 t
ORIGIN      
        1 cctgccgccc agcataatgc tcccatcccc actaacaagc agccccgcag atccaaagcc
       61 tgcatccgct cacccgagac caacaggaaa gccaccccac aaccgccgat caagagccct
      121 atccaaccgg cgagcccaag ccttgaagag tgcggcaaag ccaactcaat cagagcagta
      181 aagataggaa ctgtggaaac gatcagggct gccgcccctg aggtaaccgt ttgcaggcct
      241 aagattccaa agccgttccc gccaaagaat aaaagcagtc ccgcaccaaa catctggaga
      301 tactgcccgg cttttcgagg aaagggaagc cttttaacca atgcataaag aaggagcagg
      361 ctaccggcgg cacataaccg taaagataaa aacaataaag gcggaaaact acgaatcccg
      421 atccggttgg ccagataccc ggtaccccag atcaaacaga ttgcggcata agcgagaaca
      481 cctttgccat ctttcaaaac tctcagccac ccttatcctt tgatcgcccc gctggtgaca
      541 cccacgacga tatatttctg gaagaacata aagaaaagca ccggagtgac cgagaaaagt
      601 gacgcccctg ccaggatctg atcctgggag accacatagc tacctataaa ggaggccaaa
      661 cccacactac caatccattt gtcttggttg ctaataaaga ttaatccgta cagatattca
      721 ttccaggtga caaaaaaggt cattaccagc gttgtgacca tacccgtcat tgtcagagga
      781 agtgtgaccc ggaaaagtgc ttccatccga ttacagccat cgatgtaagc cgcttcctcg
      841 attccaaccg gaatggtgtc aaagaaaccc ttcatcaaca tcgtattcac ggcgatacac
      901 aacccggtgt cggcgatgat taatgataat gaggagtcaa tcaaccccaa tttgctgaag
      961 acaatgaata acggggtgat caccatcggg ccggaaagca tctgcgtgct cagtaatgcc
     1021 agtgataagg tttttctccc cttatatctg aatctggaaa aactgtatcc agccaaagtt
     1081 gcgaagatta cggttaatcc aacagtgctt atagtcacaa tggcggtgtt gagtatccag
     1141 cgccaaatcc ggccatcgac aaaaagttta tgaaaccctt ccacgctgaa tagtttcggg
     1201 aaaaaagccg gaggatatga gaataaggcg ctctcccttt gaaacgaact gttcaaaagc
     1261 caataaatcg gaaatgcaat aagaacgcag gtgactacca tcaatagata aaataatacc
     1321 cgcgaaagtt ttttccggaa tgtagaatcg ttcacggcca tctgtttcaa ctccccccgg
     1381 ctacttttga tcctctttgg acaggataaa gtagcctatt gtgatcaaca tcaaaaaaat
     1441 aatcatcaca gttccgattg ctccggcata ccccatctga taatatttga atgctttaag
     1501 ataaacttgg atgaccagag attcggttgc ccgtgcagga ccgccttggg tcaatgccca
     1561 gatgatagtg aaacgttgaa atgaccagat aacggtcaag agaaccaata ttcccatgat
     1621 tccggagata gacggtaaag tgacatagaa gaatttctta attccggtcg ccccatcaag
     1681 ctccgcggat tcatacagct cttccggtac accctgcaaa gcggataata aggccaggga
     1741 atgaaaggga aataaccgcc aaaccgatac cacaagaaca gcgaacattg ccacgctcgg
     1801 gttagccaac cattggacgg gttgggcaat gaggcctaac cccttcaaga tgtaattaat
     1861 cacaccgtat tgataatcaa agagccaggt ccaaacggcc accgcggcaa tatcgggaat
     1921 ggcataagga ataatgagta atgtccgggc caggggccgg cctttaaatt tggcgttcag
     1981 caagagcgct gtggccaacc cgaccccaaa aactcctacc acatagaaaa aggagtagat
     2041 gacggttatt ttacatgaat accaaaaatc cggatcgttc gtcatccgca aatagttctg
     2101 taaacccaca aaaggggctt gctctacgaa cttcgcatcg gtaaacgaga ggagaagacc
     2161 cttaataatc ggataaaaat aaaccaacgc taaaacaagc agggcgggca gaaccaggag
     2221 atacggaagg gtgtcgaatt ttctccttgc ttgaacactc acgatgtaaa cccttctttc
     2281 ttaatagaat cttcgtaaga tggtggaggg cattgtactt cccgccctcc accgaatttc
     2341 agttagttaa ataaattact tctttacgat gtcgccgaga actttctcag cgtcgttcat
     2401 cgcctgatca atgggtttat tggcgaaaaa gatttgagcc acgttatcaa ccagagttct
     2461 ccggatctgc tcgctctgtt cctcaaagcc cttcaccatg gtgggcatcg gaatcagctt
     2521 gggatcatta taagcagcca aatacggatg ctgatcaagc caagccttgg gatagttggt
     2581 ctttacggtt ccgatatgca gaccgaacat gttatattcg gcctggttgt cggcgcggat
     2641 ccaccatttc aggaatttaa cagcttccgc cttatgtttg gaggctttgg agattccaaa
     2701 gaagttctcg ttgaacagta cgtgattgcc agggaacggc ggacgtacag cgccgaaatc
     2761 attggaatgg ccgggataaa aaccgtcgat gatgccgaat tgatatgctc cgtcaaaaat
     2821 catcgcgatc ttgttatcgg cgaacatccg ccgttgtacg gaccagccga ttcccttggg
     2881 ggtgacatcg gcgtcgtaga cctttttgaa gcattcaacg ccctttacga aattgggatc
     2941 attcactgcg atcttgccat tcttggaaat tcccgcatcg aaaccgctgg cgaatttctg
     3001 aatgccttgg gcgaagtatt cggtttcggt cggcactacc ggcattccga aaccatactg
     3061 atccggggct ttcgtcaact tcttggcaac atcaatcaac tcttccggag ttgtcggagg
     3121 tttgagtccc gcatcagcca gcattttctt gttgtaaatc aattggccgt atgcgaagga
     3181 catcaagttc aaggcataac gatggccgtc aatgacacct tcgttattga tgctttccag
     3241 tttatactgg gacggtttga tatacttg
//
LOCUS       DASVMB010000233         9254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_544568,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000233 DASVMB010000000
VERSION     DASVMB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544568"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1812
                     /locus_tag="VF531_06780"
     CDS             97..1812
                     /locus_tag="VF531_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713710.1"
                     /translation="MNKRRLQSRFLLFAAALLLLLLVPAVAGALPDDPAGSSDFQHGL
                     REIGARYGVTIIVDPMLGGPVPATIQEGSIDAVLQAFLEPSGYKYTKVDDYYLVSGVE
                     SPLAVLAAADSRRVLVGFLDRKAREELGEFAPYLTYDPAGDAVFVKAPAGQLHQILAK
                     LWQIQGNVGQLAVVYNLQITDLKNSSAFDYLFSAEYDRARQEGNTWVITPDQLSSDGS
                     LRMLTEAKAERFSESVSRQPWLITLPGETVQFRSSRRDIDTGLDIERYFTVRITPLTV
                     NDTNGEVLSDIDIEQNILSHANLVESSFQASKDRIYRLDSQVKTVPGKRQFVAVVRQT
                     GEVETRSIWGLGRGKHQEHRDLIITVNATPLNLQSALATTGGLMPVASLGGMEKLNVG
                     DLPETGSRSVVTLGIGSHDAKDFVGRFELTAPLSTGIDLNLSYLGEDLYSGGFSFKMD
                     SANAFSLELVAGKGIGPDKQDAVMVGLGDLTRPVDYLSLFAKYYPYSHLFDTQEFSGD
                     PVWQAGLRLGPEHFGLSLEAAGNPDFTGWDVTLDLKTRNTTWFLDYNTRSEKALYGLG
                     VKFGL"
     gene            2144..3001
                     /locus_tag="VF531_06785"
     CDS             2144..3001
                     /locus_tag="VF531_06785"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX7713711.1"
                     /translation="MKKILVLPNPLKPEALELTPRLVRLLSNHDFFPVLEQETAALLK
                     LSEFGCIPGQLWDELDLVMVLGGDGSMLTAARRVYPRQIPLLGINLGHLGFLTWVESN
                     RLEAALQDLQCANFSLEDRTMLEARVSRNGEPLKQFVCLNDLVLAKGSFARIIQLETW
                     IDESYFTTYPADGLIVATATGSTAYSLSAGGPILDPRMDAIVVTPICAHSLYARPVVL
                     NHEARVKVVLNANHKEIVLTADGQEGVALQPGDEIIFTRASRVTRLLSLGERGPFEVL
                     QSRLKEGRI"
     gene            3047..3499
                     /gene="argR"
                     /locus_tag="VF531_06790"
     CDS             3047..3499
                     /gene="argR"
                     /locus_tag="VF531_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX7713712.1"
                     /translation="MKNNRQAMIVAIIKEKVIGTQEELGEALKARGILVTQATLSRDI
                     KELGLIKVPTLEGYYRYSLPQDRTPGDLMRRAQRMLDDAVVSIDGAENLIIIKTMSGT
                     AQGVAAAFDELEWPEVVGTVAGDNTILVVVRSKEQTTAVLDKLQQLRR"
     gene            3504..5252
                     /gene="recN"
                     /locus_tag="VF531_06795"
     CDS             3504..5252
                     /gene="recN"
                     /locus_tag="VF531_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX7713713.1"
                     /translation="MLQEIHVKNLALIEEIRLEFDPSFNVLTGETGAGKSIIIDALGL
                     ALGGRFSSEMIRTGAESATVEVTFSGENRPALNGYLESLGITPEADHSLIIQREIGVN
                     GKNRCRVNGQLVTVLSLAKVGEFLIDIHGQHEHQTLLFPEKQLELLDEYCGAECLRLG
                     RDFNKLFKDWQSLEEEYRGLLQSETDLARKIELLQFQINEISQAKLVIGEDEDLAKER
                     EILSSSEKLYEAATQSYQALYENTGGSTAVELLGNAQHTLEQAAAIDPRLASILETLR
                     EAACQAEEVSRELRTYQEQIQFDPERLSQIEQRLDEITNLKRKYGGSIGEILSFSEKC
                     SQELAAIAGREERSQQIVAELDDIKAQLGAVAVKLSAARAEGARRLEQAIMTQLNDLN
                     MSNTRFEIELTQEDAAEGIPFHDRKVTARAEGADKIEFLVSPNPGESLKPMTKIASGG
                     ELSRIMLALKAILAELDQVPTMVFDEIDVGIGGRTAQAVAEKILLIGQSRQVICVTHL
                     PQISSLAKRHFYIEKHVVGERTQVSVRELTMNERVEELARMLGGAQVTETTRQHAREM
                     LTLAEKLRLNKVGQMH"
     gene            5249..5701
                     /locus_tag="VF531_06800"
     CDS             5249..5701
                     /locus_tag="VF531_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713714.1"
                     /translation="MRPVWNRFAYWLILSLSLVTIYPTIDAQAFQDSSWISCGYQDNS
                     YSLGFFGYRIQDHAVELGALISDDTGYGLPGYGLDYLFFKDLSDETAIYGGIGLYRRY
                     YLDDTGGFKGRTEWAYSIGAQHDFFQALGLGFGYHSIRGVNLQVKLGF"
     gene            complement(5910..6077)
                     /locus_tag="VF531_06805"
     CDS             complement(5910..6077)
                     /locus_tag="VF531_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713715.1"
                     /translation="MMERMFYCVNHKSGGGFPGFRSRVWWKKVFAVVIYLFIFINITV
                     ISVNYFIRSIH"
     regulatory      complement(6187..6289)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6520..7131
                     /locus_tag="VF531_06810"
     CDS             6520..7131
                     /locus_tag="VF531_06810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7713716.1"
                     /translation="MHSTLKVVEMMKDIWLRKETMMRRSWNYRPVVSWLLPVLVVVSV
                     VGILEFQKSFAAPATAPQIGYRAPQFTLTDLNGKKMNLGTAVNRNRITLVNFWATWCP
                     PCRAEIPELVKFYGEYKTKGVALLAVNLQESPEAVKAFAGKNGMRFPVLLDSSGKVAQ
                     VYQVYAIPTTFILDRHGVIRDLIQGSINLATLQTKVGKLLAEK"
     gene            7133..7876
                     /locus_tag="VF531_06815"
     CDS             7133..7876
                     /locus_tag="VF531_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7713717.1"
                     /translation="MTNSLNFWVAFGAGLLSFLSPCILPLAPGYLAIISGASFTDLTA
                     GRGSRRRLFWTTTAFIFGFMLIFMALGLSSSYLGGLLRSGRRYLMIGGGFVVVLFGLH
                     QTGWLTVSWLYRERRIGGSALSTHTHTSTGVLGPFLSGLAFALGWTPCVGPILGSILA
                     LAGVTGDPGRGAMLLAAYSLGLAVPFFLLALVFDRAVKGLNRIKPYLGYLEKGSGVLL
                     IVMGLLLLSGGFAGISGWLLRFTGGWNPG"
     gene            8140..8742
                     /locus_tag="VF531_06820"
     CDS             8140..8742
                     /locus_tag="VF531_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7713718.1"
                     /translation="MLQLSVRQRLAALILVVLLALGGVVLFLQDGRGESQDLPARIAA
                     EPIYVHVCGAVVRPGVIQMKPGTRKLEAIRLAGGALPEADMNSVNLAEFAEDGEQIYL
                     ALQGETVPVNTPKKTSTASSVSRKSKLQPALKPSFPYDLNTVTLKELDIVPGIGPSLA
                     QKILEYRTTHGRFQSYDELLNVSGVGTAKLDKFRSYLYVK"
     gene            8739..>9254
                     /locus_tag="VF531_06825"
     CDS             8739..>9254
                     /locus_tag="VF531_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HEX7713719.1"
                     /translation="MSRYVNTLALLAAIAGILTAAVSNIAPGFWLAGSLGFLAITGPL
                     LFRNRVRWGWVLLLCIWCTAGFLFATARLNYRTELRRNQVYSGRDLTLAGVPVSRVTT
                     SEISGRVSYRFTLAIRSPGPAGRITVYSREPFPAEWYGKRVRLSGRFKAATAQGIWYP
                     GFSERKSLSGYL"
BASE COUNT         2188 a         2100 c         2545 g         2421 t
ORIGIN      
        1 ggatatcccc atcatcggct ggcttttcaa acagaagtct aaatttcgag gggatatgga
       61 actgttgctg ttcctcacgg gtgaggttgt taaggaatga ataagcgacg gttgcaaagt
      121 cggttccttt tgtttgcggc ggcgttgctc ttattgttac tggtcccagc cgtagccgga
      181 gcattaccgg acgatccggc gggcagttcc gattttcaac acgggttgag ggagatcggc
      241 gcccgttacg gggtgaccat catcgtcgat cccatgcttg gcggtcctgt tccggccaca
      301 attcaggaag gttcaataga tgcggtttta caagcgtttt tggaaccttc aggttataaa
      361 tacaccaagg tggatgatta ttatctggtg agcggcgtcg aatctccttt ggctgtatta
      421 gccgcagccg attcccgccg tgtcttggtg ggttttctgg atcgcaaagc ccgggaagaa
      481 ttgggggagt ttgctccata tctgacctat gatcctgcgg gggatgcggt ctttgtaaag
      541 gcgccggcag gtcagctaca tcagatcctg gcgaaacttt ggcagattca aggtaacgta
      601 ggacaactgg ctgtggtcta taatttgcag attaccgatc tcaagaactc cagcgccttt
      661 gattatctat tcagcgccga atatgatcgg gcccgccagg aggggaacac ctgggttatc
      721 actccggatc aattatcaag tgacggttca ctccggatgc tcaccgaggc aaaggcggaa
      781 cgtttttcgg aaagtgtttc ccgccaacca tggttgatta cgttacccgg ggaaacggtg
      841 caatttcgtt cgagccggcg tgatatcgat accggcctcg atatcgagcg ttattttacg
      901 gtccggatca ctccattaac cgtcaatgac acgaatggtg aagtactctc ggatatcgac
      961 attgaacaaa atatacttag tcatgctaac cttgttgaga gttcattcca agcatccaag
     1021 gatcgaatct atcgtttgga ttcacaggtt aagactgtac cgggtaagcg tcagtttgtc
     1081 gcggtagtac ggcagaccgg ggaagttgaa acccgttcca tttggggatt gggaagagga
     1141 aaacatcagg aacaccggga tctgatcatc acagtcaacg cgaccccgtt gaaccttcag
     1201 tcggcgttgg caactacggg tggattaatg ccagtggctt cattaggcgg tatggaaaag
     1261 ctaaacgtcg gagatcttcc cgaaacaggt tctcgatcgg ttgtgacact cggaatcgga
     1321 agccatgatg caaaggattt tgttggtcga ttcgaattaa cggctccgct cagtaccgga
     1381 atcgacctca acttaagtta tctaggggaa gacttatatt ccggcgggtt ttcctttaag
     1441 atggattctg cgaatgcatt ttcccttgaa ttggttgcag gtaaaggaat cggtcccgat
     1501 aaacaggatg ccgtcatggt aggattggga gatctaaccc ggccggttga ttatctcagt
     1561 ctttttgcca aatattatcc ttactcccat ctctttgata cgcaggaatt ttccggtgat
     1621 cctgtttggc aggcaggctt aaggctggga ccggaacatt tcggactttc cctggaggcc
     1681 gcaggtaatc cggactttac cggatgggat gtgaccctgg acctgaagac ccgaaataca
     1741 acctggtttt tggactacaa tacccggtcg gaaaaagcgc tctatggact aggagtaaag
     1801 ttcgggctat gaaattgaaa gcaatccatc ctttatcagt ccccttaccg cagagttacg
     1861 gaagggactt tttttatatg gttccaattt gacgtttatt ttccatcagt atctataaat
     1921 ttccggtgat gttccatttc aaattttcgt ggtaaagatt cctgattacc ggtttgaacc
     1981 ggaaggaaat atgaaattta taaggaataa atataggggg ataatgaaga atttccgggt
     2041 ttcaactgcg gtggagcatt aatccctttt ttacccgaaa atcattcctt tacttaatgg
     2101 attctttaat aaaatctcga tctccaattg gttggtgtga tgcttgaaaa aaatcttggt
     2161 gcttcccaac ccgctaaaac cggaagcgct tgaactcacc ccgcgcctag tccggttact
     2221 ttcgaatcat gacttctttc cggttttgga acaagaaacg gcggctttat taaaattatc
     2281 cgaattcggt tgtattcccg gtcaattatg ggatgaactc gatcttgtaa tggtgctggg
     2341 tggagacgga tcaatgctca ccgcggcgcg acgcgtttat ccgcggcaga ttccattact
     2401 cgggattaat ttaggacatt tgggcttttt gacctgggtc gagagtaaca ggttggaagc
     2461 ggccctacag gatcttcagt gcgccaattt tagcctggaa gatcggacca tgctcgaagc
     2521 ccgggtttcc aggaatggag aacccctcaa acaattcgtc tgtttgaatg atctggttct
     2581 tgccaaaggc tcatttgcca ggatcattca gctcgaaacc tggattgatg agagctactt
     2641 cacaacctat ccggcggacg gcttaattgt tgctaccgct accggttcaa ccgcctattc
     2701 tttgtcggcc ggggggccga tcctggatcc ccgaatggat gcgatcgtgg tcacccccat
     2761 ttgtgcgcat tcattatacg cccggccggt tgtcctgaac catgaggccc gggtgaaagt
     2821 ggtgctaaat gccaatcaca aagagattgt attaaccgca gacggccagg agggcgttgc
     2881 cctgcaaccc ggggatgaga tcatatttac cagggcgtcc agggttacac gcctcttgag
     2941 ccttggtgaa cgaggacctt ttgaagtttt gcaatcgcgt ctcaaggaag ggcgcattta
     3001 aatcacaggc cgtaatcggc ttttcagagt cttcgaggag gaagtcatga agaataatcg
     3061 gcaggcgatg atcgtcgcca tcatcaagga gaaggtaatc ggaacgcagg aggagttggg
     3121 ggaggctctg aaggctcgtg ggatcctggt tactcaagcc acactatccc gggatattaa
     3181 ggaattggga ctgatcaaag ttcctacgct ggaaggttat tatcgctact cgcttcccca
     3241 ggaccggact ccgggcgatt taatgcgccg ggcgcaacgg atgcttgatg acgcggtggt
     3301 ttccattgat ggggcagaaa atttgattat catcaaaacc atgagtggga ccgctcaggg
     3361 agtggcagca gccttcgatg aacttgaatg gccggaagtg gtgggaacag tggccggaga
     3421 caacaccatt ctggtagtgg tacgcagtaa agaacaaaca accgcagtac tagacaaact
     3481 ccagcagcta aggaggtagc gagttgcttc aggagataca tgttaagaat ttagccttaa
     3541 ttgaagagat tcggttggag tttgatccca gctttaatgt gctaaccggt gaaaccggcg
     3601 ccgggaaatc gatcatcatc gatgcgctgg gtttggcttt gggcggaaga ttcagttcgg
     3661 agatgattcg gaccggagcc gaaagcgcca cggttgaggt gacattcagc ggtgaaaacc
     3721 ggccggcgct caatggttat cttgaaagtc tgggcattac accggaggcg gatcattctc
     3781 ttatcatcca gcgggagatc ggggtaaatg gaaagaatcg ttgccgggtt aacggtcaac
     3841 tggtaaccgt actctccctg gcgaaagttg gagaatttct tattgatatt catggtcaac
     3901 atgagcatca gaccttacta tttcccgaga agcagctgga actgttggac gagtattgcg
     3961 gcgcggaatg cctgaggttg ggcagggatt tcaataagct attcaaagac tggcagtccc
     4021 tggaggaaga atatcgcggt ctgctccaga gtgaaacgga tttggcacgc aagattgaat
     4081 tgctgcaatt tcagatcaac gaaatcagcc aagccaagct ggtcattggt gaggacgagg
     4141 atttggccaa agaacgggaa atccttagct cgtcagagaa gttgtatgag gccgctaccc
     4201 aatcttatca agcattgtat gagaatacgg gcggcagcac cgcagtggag cttttaggaa
     4261 acgctcagca tacgttggag caagctgcgg cgattgaccc ccggttggct tcgattttgg
     4321 aaaccctacg tgaggccgct tgccaggcgg aggaggtctc ccgggagctc cgtacctatc
     4381 aggagcagat tcagtttgat cccgagcgct taagccaaat tgagcagcgt ttggatgaga
     4441 tcaccaattt gaagcggaaa tacggcgggt ccatcggcga gattctttcg ttctccgaaa
     4501 aatgctccca ggaattggcg gcaatcgctg gccgtgagga acgttcccag cagatcgttg
     4561 ctgaattgga tgacatcaag gctcaattgg gtgcagttgc ggtcaagctc tccgcagccc
     4621 gggctgaggg tgccagacgc ctcgagcagg ctatcatgac gcagttgaat gatttgaata
     4681 tgagcaatac ccgctttgag attgaactca ctcaggaaga tgcggcagaa ggcattccat
     4741 ttcacgatcg gaaagtgacg gcccgggctg agggggccga caagatcgaa ttcttagtat
     4801 cgccgaatcc cggtgagagc ttgaaaccga tgacgaagat tgcctcgggc ggtgaattat
     4861 cccggattat gctggcttta aaagcgatcc tggccgagct cgatcaggtt ccaacgatgg
     4921 tctttgacga aattgacgtg gggatcggcg ggcgaaccgc tcaagccgtg gcggaaaaga
     4981 ttttactcat tggccagtcg cggcaggtga tctgtgtgac ccatttgccc caaatctcga
     5041 gcctcgcaaa gcgccacttt tatatcgaga agcatgtggt cggagagcga acccaggttt
     5101 cggtccggga gttaaccatg aacgagcggg tcgaggaact tgcgagaatg ttgggcggcg
     5161 ctcaggtcac cgagactacc cggcagcatg cccgggagat gttgactctg gccgagaagt
     5221 tgcgattgaa caaagtgggg cagatgcatt gaggcctgtc tggaaccggt ttgcttattg
     5281 gttgatcttg agcctgagcc tcgtaactat atacccaact atagatgctc aggcttttca
     5341 agattcttcc tggatatcct gtggctatca ggataacagt tatagtttgg gcttttttgg
     5401 ttaccggatt caggatcatg ccgttgaact tggagcgttg atttcggatg atactgggta
     5461 cggtctcccc ggttatggtc tggattacct tttcttcaag gatctttcgg atgaaacggc
     5521 gatctatggc ggcatcggcc tttaccggag atactatctg gatgataccg ggggctttaa
     5581 gggccgaacc gaatgggcgt attccattgg cgcgcaacac gatttttttc aagctctcgg
     5641 tctgggcttc ggatatcatt cgattcgcgg agtgaatctt caagtaaagt tagggtttta
     5701 aagggagata caagccccat ggatggggtg ggataatact gcgaatttta tccgagcccg
     5761 cactaaatta cgaaaccaca cgaaaaaact aaatgactat agactttttc gtgtggtttc
     5821 gtgtgaagag ttctcctttt atgcggataa tgaccgaaat taatgagaat tccaagatgg
     5881 acaccctttg atttatccaa ccggcaagtt taatgaatcg agcgaatgaa ataattgact
     5941 gaaataactg tgatattgat aaaaatgaag agataaatca ccactgcaaa aacctttttc
     6001 caccataccc tggaccggaa cccggggaat cctcctcccg atttatggtt gacacagtaa
     6061 aacatgcgtt ccatcatcca tccctccgta atctggttga gtttcatttt tgtagcgaac
     6121 ttcaggacga aatcgttggg ttgtcgtgtt aaattaaccg attgaggaaa gtaaaaaaca
     6181 actcatcgag gcggcccggc gatcgtgggg acggtttttt atcgcaccat ttcccgttag
     6241 acccgtggct ttgcgtctct ggctttcgcc agatttgccg ttaacagaat ttgttgttta
     6301 tcatcacgat cttaccatcg aaaacagttc cttgcaagtt aaatactgag atggcaggaa
     6361 gaacatgaaa cgggttgaca aaacgtagaa taatctagat ttacgtatgt taatccattc
     6421 ccttcttgac tatagtcgat tcttgcttta tactatacct agtatggtat ataatcaaca
     6481 ttttccattg tcagtcgcta ttgaaaaggg aatttcatca tgcacagcac gttaaaggtg
     6541 gtagagatga tgaaggatat ttggttgcga aaggagacca tgatgaggcg aagttggaat
     6601 tatcggcctg tggtgagttg gttgctcccg gttttggttg tggtttcagt cgtaggcatt
     6661 ttggaatttc aaaaaagttt tgcggcaccg gctacagcac cccaaatcgg ataccgggcc
     6721 cctcagttca ctcttacgga tctcaacggc aaaaagatga atctcgggac tgcggtcaac
     6781 cgcaaccgga taacccttgt caacttctgg gcaacctggt gcccgccgtg ccgggctgag
     6841 attccggaat tggtgaagtt ttacggtgag tacaaaacca agggtgttgc attattggct
     6901 gtcaatctcc aagaatcccc ggaagctgtg aaggcgtttg ccgggaagaa cgggatgcga
     6961 tttccggtgc tgctcgacag ttccgggaaa gtagcccagg tttatcaggt ttatgcgatt
     7021 cccaccactt tcattttgga ccgccacggt gtgattcgcg atttgattca aggcagtatc
     7081 aacctggcca cgcttcagac gaaggtcggg aaactattgg cggagaagta aaatgacgaa
     7141 ttccctcaat ttctgggtgg cctttggtgc cgggctgctt tctttccttt cgccctgtat
     7201 cttaccacta gccccgggtt atctggcaat catttccggg gcttcgttta ccgacctcac
     7261 ggccggacgg ggttcccggc gccggttgtt ctggactacc accgctttta tctttggttt
     7321 tatgctgatt ttcatggcat tgggcctttc cagttcatat ctggggggat tactccggtc
     7381 aggaaggcgt tacttaatga tcggcggcgg tttcgtggta gtcctcttcg gtctgcacca
     7441 gaccggatgg ttgaccgtct cttggctcta ccgggaacgg cgaatcgggg gttccgctct
     7501 atctacgcat acacatacat ctaccggagt tttgggccca ttcttaagtg gtttggcttt
     7561 tgctctgggt tggacgccgt gcgttggccc aattctcgga tcgattttgg cattggcggg
     7621 tgttacgggt gatcccggcc ggggagcaat gctgctcgcc gcatactctc tggggctggc
     7681 ggtgccgttc ttcctgttgg cgttggtctt cgatagggcg gtaaagggac tgaaccgtat
     7741 aaagccgtac ttagggtatc tggagaaggg gtcgggggtt ctgctgatcg tgatggggct
     7801 acttttactg agcggcgggt ttgccgggat tagcggttgg ttgctccgtt tcacgggcgg
     7861 ttggaatcca gggtaaaatc aaagtttaaa tggataggga aaagggtaaa aattgatagg
     7921 gcaacgggca acaggcaacg tctcatttct taaccgcatc gattgcaccc gcatactatt
     7981 gcctgttccc tgttgcctcg cgtctcctga gacgcgctgc ctcacgcctt gtttaacaaa
     8041 ttttccccaa aatgaaggta taatttataa aatattgaat tgttaatcaa ctaactcaac
     8101 aacttctcca gaagatattt ttgtggggag gcggtgcggg tgttgcagtt atcggtccgg
     8161 cagcggctgg ctgccttgat cctggtcgtg ttgttggctt tgggaggagt cgtcttgttt
     8221 cttcaagatg gccgggggga gagtcaagat ttgcccgccc gtattgcggc ggaaccaatc
     8281 tatgtccatg tctgcggcgc ggtggtccga ccgggggtga tccaaatgaa accgggaacc
     8341 cggaagcttg aggcgattag actcgctggc ggggcgttac ctgaagccga tatgaactcg
     8401 gtgaatctag cggagtttgc cgaggatggg gagcagatct acctggccct ccagggtgag
     8461 acagtgccgg ttaacacccc gaaaaagaca agcactgcat catcagtatc gaggaaaagt
     8521 aagctccagc cggcacttaa accatctttt ccttatgatt tgaacacggt cacccttaag
     8581 gagctggata ttgttcccgg tatcggtccc agtctggccc agaagatcct ggaataccgg
     8641 acaacccatg gcagatttca gagctatgat gagcttttaa atgtctcggg agtcggtacc
     8701 gccaagcttg ataagttccg ttcttatcta tatgttaaat gagccgctat gtaaacaccc
     8761 tggcgttgtt ggcggctatc gccgggatat tgaccgcggc cgtctcgaat attgcccccg
     8821 gtttttggct tgccggcagc ctgggttttc tcgccatcac cggtccgcta ttgtttcgga
     8881 accgggtgcg atggggttgg gtattgctgt tgtgtatctg gtgcacagcc ggttttctat
     8941 tcgctaccgc acgtttaaat tacaggacag agctacgccg gaatcaagtc tattccggca
     9001 gggatcttac ccttgccgga gtgccggtta gccgggttac gacatcggaa ataagcggac
     9061 gcgtaagtta ccggtttaca ttggctatcc gttccccggg accggcgggt cggattactg
     9121 tttacagccg ggaaccattc cctgccgaat ggtatgggaa aagggtgagg ctctcgggta
     9181 gattcaaggc ggccaccgct caaggcatat ggtatccggg attctcggag cgaaaatccc
     9241 tcagtggtta ttta
//
LOCUS       DASVMB010000234         6841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_174638,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000234 DASVMB010000000
VERSION     DASVMB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174638"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF531_06830"
     CDS             <1..325
                     /locus_tag="VF531_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX7713720.1"
                     /translation="AEVNRISNLYHPWLNLLTETAQTMPSDLWLTSFKGTAQGSITIT
                     GNTVRFTAIGDLVRRLQETAMIQNPGARVQVKEIRRLTMKGKTGYQFILEIETGRAAL
                     EYSER"
     gene            306..893
                     /gene="pilO"
                     /locus_tag="VF531_06835"
     CDS             306..893
                     /gene="pilO"
                     /locus_tag="VF531_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7713721.1"
                     /translation="MNTPKDKGQYLGPTIVLGVFLLAVSGGFILHQQNRNLARELHKK
                     EAQLSSARRQLRSYRSNDTQKRRIELEHLRLEEFIPSRENQEEMIMELGQLAQKYNLK
                     ISRCVLNPKTLILKELPQYQASQWLVELNGDYQGLTNFLAALPQGSRLILVSGMEIAS
                     VAQSDLNGQPLDAEYQLDARVTLDLISNLTEKVSP"
     gene            890..1741
                     /locus_tag="VF531_06840"
     CDS             890..1741
                     /locus_tag="VF531_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7713722.1"
                     /translation="MKSGPKHSPSHSIFKTVFWIIAGISLLAIGMLIFLALSRESFSS
                     ISGHSPKAKPEKAPLQAVTDVETGQSSPEATPIQSRDPFQPPAGAGTEWRKATGGAII
                     QSVAGSKIKSSEQPIPTVGKPRSKPKRTPIPSPTLPLASPSPDPSIGINGDAPPEEKE
                     IPVLPGPNQSGVAYQAVYRVKAGDSVFQIARKYGVSAEVIRKANQLASIGSKLVAGTE
                     LVVPVPPDHLYRLKAGETIWRLAMRYGIPLVQLVEINQITDQSQLETGRLIILPVPVT
                     RIRNAKY"
     gene            complement(1791..1982)
                     /locus_tag="VF531_06845"
     CDS             complement(1791..1982)
                     /locus_tag="VF531_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713723.1"
                     /translation="MDPISKPGAADNAENAVRCSHCGNTILIGSEDGTDQKEVECDVC
                     HTTQAIHPDQVRGASNEDY"
     gene            complement(2027..2572)
                     /locus_tag="VF531_06850"
     CDS             complement(2027..2572)
                     /locus_tag="VF531_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713724.1"
                     /translation="MRTPKSILGIGIMVISLVIGGCSLFPSNQTRPAPVRPRATVSPR
                     SSPAPLGPIATRGPKTTIKGKKPTTKVRPAPVRTPGLSSVTDQHLVNRLVTINNAVTK
                     NNWTVANREVNTLGTDMARFRPTRSKAKTLRDMASFDTIYVKLQADVKAKNKTAAMQD
                     VKNMQNALRDLKKSPTSPSSR"
     gene            2786..3313
                     /locus_tag="VF531_06855"
     CDS             2786..3313
                     /locus_tag="VF531_06855"
                     /inference="COORDINATES: protein motif:HMM:NF041644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO0543 family protein"
                     /protein_id="HEX7713725.1"
                     /translation="MAYILTTIALWVYFALAVPLRNWRHYYPTLIFSALLGTISDLLG
                     VVLDQWAYYGPKVGGLSLWSDLGIAPAEGGIFIRMMPAGRWSRLRFPYWILWAIVNAV
                     FEWFFVWVGWIGYDHWNPFRAFLFYLFFFGCIWVQEQWYQGTGRLGKRTGADLKNDKK
                     VRGNGDKSPGGRGSD"
     gene            3328..4581
                     /locus_tag="VF531_06860"
     CDS             3328..4581
                     /locus_tag="VF531_06860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713726.1"
                     /translation="MESYCFRPAQPGDRDGLLDLIEAGFGSQTAKFDPQTGMEHRTLF
                     SYLYSLPGHDFHRIMLATDDDSTLAAAGALPQQLWMGEVPVPVWAISPVVTAPGERGK
                     GLAGQCLIRLLAHLKDGNIPAVFLWGIPGFYPKFGFVPILPRFKTKISHEQLHPGFPK
                     IQGELRPFAAEDLPEIAALYDLGNQIYWLQPRRNMDWWQGRAAEMNTPQGYIKEVPFP
                     VSDHFLVWENQAREVSGYMYYETEPTHRRLVVLEGAALDPETAMMMLVRFIHLYLPPG
                     QSLLIRGTPNHVLNLAAYRLGGIHQNPAPLAGMIKVLDWQRLLGFLKPLIDRRGSGFE
                     KLNCTWQFRIGDAIIKLVWSGSGFDWELVPTGAESGLFGNEIQLTRLVFGLYDRMDLP
                     EFNTETLTRLFPLQAPFIWDNNYLY"
     gene            4699..5832
                     /gene="nagA"
                     /locus_tag="VF531_06865"
     CDS             4699..5832
                     /gene="nagA"
                     /locus_tag="VF531_06865"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7713727.1"
                     /translation="MGQYALTKAAMVTPFNLIEDAVVIVDGDKILAAGPAITTAIPSG
                     FREVPLEGLLIAPGFIDQHLHGGGGHDVMTGTAECLAEIAKFHATHGTTAFLATTVSA
                     SPEHLIKIAGAYASLNNTPYKGARCLGLHLEGPYLSSKYAGATDTGLLRNPSFGEIQS
                     LQRISNSGIRLVTMAPELDGAIAVAQSLTKSGIACAIGHSDADYELTVKAINTGFGSV
                     THCFNQLHPLDHQEPGAMGAALTRPELVVELIADGIHIHRSILDLVWRLKGADGIILV
                     SDAMMPVGMPQSALPVNGGKPAGSSLTLESAIRKMMESTDCGLTDVFRMVTYNPARLL
                     GINKHKGSLYPGKDADLVVLTTDLEVVMTMVEGEVISGLIRLD"
     gene            5912..6553
                     /locus_tag="VF531_06870"
     CDS             5912..6553
                     /locus_tag="VF531_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HEX7713728.1"
                     /translation="MGNFESIQAAIIAGDSVTVKQLVTDDLSKGISAADIIAQGLIGG
                     MNVIAERFRNNEIFVPEVMLAAKAMQAGLTLIKPLLAGTVHQPLGKIIIGTVQGDQHD
                     IGKNLVGMMFEGAGFAVTDLGSNVAVEKFIAAVRENKPDILALSALLTTTMPRMAETI
                     QRLKDEGLREGVKVMIGGAPVSESYAVKIGADGYAPDAGAAVAVAKSLMGVTL"
BASE COUNT         1665 a         1546 c         1815 g         1815 t
ORIGIN      
        1 ggcagaagtc aaccggattt caaatctgta tcatccctgg ttgaatttgc tgaccgaaac
       61 agcacaaacc atgccctccg atctttggct gaccagtttt aaaggtactg cccaaggttc
      121 gatcacaatc accggaaaca cggtgaggtt tacggccatc ggcgatctgg tgaggcggct
      181 tcaggagaca gcaatgattc agaatccggg tgctcgcgtg caggtcaaag agattcggcg
      241 cttgacaatg aaggggaaaa ctggatatca attcattctg gagattgaaa ccgggagggc
      301 tgcgcttgaa tactccgaaa gataaaggcc agtacttagg tcccaccatc gtcttgggcg
      361 tttttctcct ggcggttagc ggaggtttca ttctccatca acaaaaccgc aatctggcaa
      421 gggaactcca caagaaagaa gcccaactga gttcggcccg gcggcaacta cgatcctatc
      481 ggagcaatga tacccaaaaa cgccggattg aactggagca tcttcggttg gaggagttta
      541 ttcccagtcg cgaaaatcag gaagaaatga tcatggaact ggggcaactg gctcaaaaat
      601 ataatctcaa gatcagtcgt tgtgttttaa atccaaagac cttgattctt aaggaacttc
      661 ctcaatatca ggcttcacaa tggctcgttg aactcaatgg ggattatcaa gggttaacca
      721 atttcctggc ggctttaccc caaggctccc ggttgatttt agtctccgga atggaaatcg
      781 cctcagtcgc ccagtccgat ctgaatggac agcccttgga tgccgagtac cagttggatg
      841 ccagagtaac acttgatcta atttccaacc taacggagaa ggtgtcccca tgaaatccgg
      901 gcccaaacat tcgccgtccc attcgatctt caagaccgtc ttttggatca tcgcggggat
      961 cagcttactc gcaatcggga tgctgatttt tttggcgcta tcccgggaat cgttctcctc
     1021 catttcggga cattccccga aagccaaacc ggaaaaggcg ccgctacagg cagtaaccga
     1081 tgtggagacc ggacagtcaa gtcccgaggc aactccaatc cagtcccgcg atccgtttca
     1141 accgccggcc ggagcgggga cagagtggcg gaaggctacc ggtggcgcta taatccaatc
     1201 ggttgccggc tcaaaaatca aatcttctga acagccaata ccgacggtcg gtaaaccccg
     1261 ttcgaagccg aaaaggactc caataccgtc gccaaccttg cctttagctt cgccgtctcc
     1321 tgatccctcc atcggtatta atggagatgc tccgccggaa gagaaagaaa tcccggtgct
     1381 tcccggacca aaccagtccg gtgttgctta tcaagcggtt tatcgagtta aagcagggga
     1441 tagtgttttt cagatagccc gtaaatacgg agtgtccgcc gaagtgattc gtaaggccaa
     1501 tcaattggcg tccattggtt caaagctggt tgccggtact gaattagtgg ttccggtacc
     1561 acccgatcac ctctaccggc ttaaggcggg cgaaactatt tggcgtctgg ccatgcgtta
     1621 tggtattccg ctggtacaac tggtggagat taaccagatt accgaccagt cccaactgga
     1681 aaccggcaga ctaatcattt taccggttcc agtaacccga attcgcaatg ccaaatatta
     1741 gacaaaataa aaagaactgc tttgcagttc tttttattta tcggatgttt ttaataatcc
     1801 tcgtttgatg ctcctcggac ttgatcggga tgaatcgctt gagtggtatg gcagacgtcg
     1861 cactccactt ctttttgatc ggttccgtcc tcgcttccga ttaagatcgt attaccgcaa
     1921 tgagagcatc gcacggcgtt ttcggcgtta tcggctgccc caggtttgct gattgggtcc
     1981 atgaaactcc cctccggata aggttttgga ttaattcctt tcaaacctac ctagaactcg
     2041 ggcttgtggg agatttttta agatcgcgca aagcgttttg catgttcttg acatcctgca
     2101 ttgcggcagt tttatttttg gctttgacat cggcttgaag tttgacgtag atcgtatcaa
     2161 agctggccat gtctcgtaag gtcttggctt tggatcgggt cggacggaag cgggccatat
     2221 ccgtacctaa tgtattgacc tcacggttgg cgactgtcca attatttttt gtcacagcgt
     2281 tgttgatcgt gaccagtcga ttgaccaggt gttgatcagt aacgctgctg agacccggag
     2341 ttcgaaccgg cgccggtctg actttggtgg ttggtttctt ccctttgata gtcgtcttcg
     2401 gccctctggt ggctattggt ccaagcggcg ccggcgagct cctgggagaa actgtcgctc
     2461 tcggccgcac cggcgcaggc cgggtttgat tgctcgggaa taaagagcag ccgccgatga
     2521 ccagacttat aaccattatg ccgatgccta agatactttt aggagtacgc atctttttca
     2581 cctccttagt agttgttctt aggttaccat ggaccgtatt ttttcaaaca tcatttatgg
     2641 gtaaaaccgt tgggaaacct ggaaatcaat tgttcatccg gtttagtctc ttaggactgt
     2701 cggtttatcc tggtggcggc aggaaaaata cgggaattta gcgaagtata attcaataaa
     2761 aatcagatcg ggcaataata aggccatggc ctatattctt actacaattg ccctatgggt
     2821 ttatttcgca ctagccgttc cgcttcggaa ttggcgccat tattatccga cattgatttt
     2881 ttcggcttta ctggggacaa tctccgatct gttgggagta gtgctggatc aatgggccta
     2941 ttacggtcca aaagtcggcg ggttgagtct atggtcggat ctgggcatcg ctcccgctga
     3001 gggcggtatc ttcattcgta tgatgccggc aggtcggtgg tcgaggttac ggttccctta
     3061 ttggatactt tgggctatag tcaatgccgt attcgaatgg ttttttgttt gggtgggctg
     3121 gattggttac gatcattgga atccgttccg cgcttttctt ttttacctct tttttttcgg
     3181 ttgcatctgg gtacaggaac aatggtatca gggtaccggc cgattgggaa aacgcacggg
     3241 agcggatctt aaaaatgaca aaaaagtccg cggcaatggg gataagagcc ccggtggccg
     3301 tggaagcgat tgaacaggtg aatttagatg gaatcttatt gttttcgccc ggcccaaccg
     3361 ggggatcggg acggattact cgatttgatc gaggctggtt ttggatcaca aacagcaaaa
     3421 ttcgatccgc aaaccgggat ggaacacagg accctttttt cttacctgta ttctttaccg
     3481 gggcatgatt ttcaccggat catgcttgcg accgatgatg attcgacgtt ggccgctgct
     3541 ggggctttac cgcaacaact ctggatgggc gaggttcccg ttccggtctg ggccatctct
     3601 ccggtagtaa ccgctcccgg agaacgcggc aagggtttgg ccggccagtg cctgattcgg
     3661 ctattggccc accttaagga tgggaatatt cctgcggttt ttctatgggg aatacccggt
     3721 ttttatccaa aattcggttt cgtgccgatt ttgccgcggt ttaaaaccaa aatcagtcat
     3781 gaacaattac acccgggttt tcctaaaatt cagggggagt tgcggccgtt tgccgcggag
     3841 gatctgcccg agattgccgc cttatatgat ttgggtaatc aaatctattg gcttcagccc
     3901 cggcggaaca tggattggtg gcaagggcgg gccgcggaaa tgaatacacc ccaggggtat
     3961 ataaaagaag ttccctttcc ggtttccgat cattttttgg tgtgggagaa tcaagcccgg
     4021 gaagtctccg gttacatgta ctacgagacg gaacccactc accggcgatt ggtggttctt
     4081 gagggcgcgg ctttggatcc ggagacggct atgatgatgc tggttcgttt cattcatctt
     4141 tatctgcctc cgggacaatc acttttaatt cgcggaaccc cgaatcatgt gttaaatttg
     4201 gcagcttatc gcttaggagg gattcatcag aacccggctc cgctggccgg aatgatcaag
     4261 gtgctcgact ggcaaaggct attgggtttt ctgaaacctc tcattgatcg tagagggagc
     4321 ggatttgaaa agctgaactg tacctggcag ttccggatag gcgacgccat tataaaactc
     4381 gtttggtccg ggagcggttt tgactgggaa ctggtgccga ccggagctga atcaggcctt
     4441 ttcggaaatg agatacagtt gacccgcctc gtttttggtc tttatgatcg gatggatcta
     4501 ccggagttta atactgaaac actgacgcgg ttatttccgt tgcaagcccc atttatatgg
     4561 gataacaatt atctttatta aatgaaatga attttgataa aatcacccgg gggttaccta
     4621 ataaggaatg agctccgacg cgattatatc tattttagga gtcaaggaat tggccaatca
     4681 ttcaaggagg ctcggaattt gggacaatat gctttgacca aggcggcgat ggtcacgccg
     4741 ttcaatctga ttgaagatgc cgtggtgatc gtggatggcg acaagatttt agcagccggg
     4801 ccagccatca ctaccgctat tccttccggt ttccgcgagg taccgctaga ggggctactt
     4861 attgcacccg gctttatcga ccagcatttg cacggcggcg gaggtcatga tgtcatgacc
     4921 ggtactgcgg aatgcctcgc tgagatcgcc aaatttcatg ccactcatgg aaccacggct
     4981 tttttggcca cgaccgtctc cgcatctccg gagcatttga tcaagatagc cggggcatat
     5041 gcatcattga ataatactcc ttataaagga gcccggtgtt taggtttaca tcttgaaggg
     5101 ccatatcttt caagtaagta cgccggggcc acggataccg gtttgttacg aaacccttcg
     5161 tttggggaaa ttcaatcact gcaacgaatc agtaattccg gaatccgttt ggtaaccatg
     5221 gcccctgaat tggatggagc gattgccgtg gcccaatcac taacgaagtc tggcattgct
     5281 tgtgccatcg gccatagcga tgcggattat gaattaaccg tcaaggccat caacaccggt
     5341 ttcgggagcg taactcactg tttcaatcag ttacatccct tggatcacca ggaaccgggc
     5401 gcgatggggg ccgcccttac ccgaccggaa ctggtagtgg aattgatcgc tgacggtatc
     5461 catattcacc ggtcgatttt ggatttagta tggcgcttga agggagctga cggaatcatt
     5521 ttggtttccg acgcaatgat gccggtggga atgccgcaat ctgcacttcc ggtgaacggc
     5581 gggaagccag ccggcagttc gttgacactg gaatcggcca ttcggaaaat gatggaaagc
     5641 accgattgcg gattaacgga tgtatttcga atggtcacct ataatccggc acgacttttg
     5701 gggattaata aacataaggg gagtttatat ccggggaagg atgcggatct tgttgtttta
     5761 accactgact tggaggtggt tatgacgatg gttgagggtg aagtgatcag cggactgata
     5821 cggctggatt aaattcaagc aaatgccttt cttttagaga gttcaattca ttatatatga
     5881 atgcaccact ataattgggg agtgtgggta agtggggaat tttgaatcga ttcaagcggc
     5941 gattattgcg ggagatagtg ttactgttaa gcagctcgtt accgatgatc tctcgaaagg
     6001 aatatccgct gccgatatca tcgctcaagg tttgattggt ggaatgaatg tgattgcgga
     6061 acgatttagg aataatgaga tttttgttcc cgaagtgatg ctggcagcaa aggctatgca
     6121 agccgggtta acgctcatca aaccgctact ggccgggaca gtccatcaac cgttgggcaa
     6181 aattatcatt ggtactgttc aaggagatca gcacgatatc gggaaaaact tagtggggat
     6241 gatgtttgag ggagcgggtt ttgcagtgac ggatctagga agcaacgtag cggttgagaa
     6301 gtttattgcc gctgttcggg agaacaaacc cgacatcttg gcgttgtccg ccttgttgac
     6361 cacaacaatg ccgcgtatgg ccgagacgat tcaacgtcta aaggatgagg ggttgcgcga
     6421 gggtgtcaaa gtcatgattg ggggggctcc agtctctgag agttatgctg ttaaaattgg
     6481 ggccgatggt tatgcgccgg atgcgggtgc cgctgttgcg gtagctaaaa gtcttatggg
     6541 agtaaccttg taaccaatca caccggtttt tttgaaacaa actattgact ttgtcacagg
     6601 gatatgataa attattaaag ctgtcgcgat tgagtaattt gcgaacgaaa aggaattgaa
     6661 aaagtttctg aatagaacaa attaccattg acagtaactt aatgagtttg atataataac
     6721 taatgcgtcg cggaactgag cttcaggaaa gcgaccaatc aagcaccttg aaccttgaaa
     6781 actgaacagt agaaagaaaa tggtaggtta aagcaagaag aaataacctg tcaagcacag
     6841 a
//
LOCUS       DASVMB010000235         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_322579,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000235 DASVMB010000000
VERSION     DASVMB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322579"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VF531_06875"
     CDS             <1..539
                     /locus_tag="VF531_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX7713729.1"
                     /translation="FAGTNLIITGATLLEITATTLVVNIRYILMSFSLSQKVAERITL
                     PERCLLSFGITDETFTVASMHKEKITFSYMLGLITGPYWGWALGTLLGAMICSVLPET
                     LQNSMGIALYAMFIALVVPNAKKSRAALAVVFIAVAISSAFTWVPYLHGISQGWGIIL
                     ATIAACLVGAIFFPREEE"
     gene            542..859
                     /locus_tag="VF531_06880"
     CDS             542..859
                     /locus_tag="VF531_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX7713730.1"
                     /translation="MAKVLIAVLLMAVVTYIIRVLPLAFFRSQLRSRFLRSFLYYVPY
                     AVLGAMTFPAILYSTGNLYSAWIGMGVALVMAFFESGLLRVALGAIVSVFLAEWVIRL
                     ISF"
     gene            complement(890..1315)
                     /locus_tag="VF531_06885"
     CDS             complement(890..1315)
                     /locus_tag="VF531_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7713731.1"
                     /translation="MFPNWVYFALGSAFFAGLTALFGKIGVTGINSNLATFLRTIVVL
                     IFSGSIVFATGEWQNPAKLPGRTLTFLILSALATGASWLCYYRALQLGPASRVAPIDK
                     LGVVFAMLLAFIFLGEKADFKTLSGGVLIVAGALIIALK"
     gene            complement(1374..>2545)
                     /locus_tag="VF531_06890"
     CDS             complement(1374..>2545)
                     /locus_tag="VF531_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7713732.1"
                     /translation="YAFIATLGSVLGGLFGYPLGVRLGRPLLIRFVDEVRLKRVEGFY
                     HRYGNWATVIGGLTPIPYKIFAIAAGVFRTRLAGFIPASIFARGIRFFIEAILLIRYG
                     RGITKLLQTTFSPVHLIWLLISLTIIFLFWKTGLKNGIFLRFMRNLVKKWLSWAERLR
                     RNYLPAGMFGWYLITGACLTGFGFMIFTKLASELLERELTHFDTTIRAVIIAFRTAWL
                     TWLMRGITNLGSTGWIIGLLLVLLGIGFRIRRRLEVLVLNLSVGGALLLSELLKISFH
                     RHRPDLPWLTSANGYSFPSGHSLISMALYGFLAYLVFRNSRRPGLNRVIGIGLVLLPV
                     LIGISRVYLGVHYPSDVLAGWSVGLAWVGTCIVGMESLATHLADLPPSGGREREG"
BASE COUNT          631 a          639 c          664 g          611 t
ORIGIN      
        1 aattcgccgg aaccaacctg atcattaccg gtgcgacgct gcttgagata actgcgacca
       61 ccttggtggt gaatatccgc tatatactaa tgtcgttttc actctcacag aaggttgcgg
      121 aacggatcac cctgcccgag cggtgcctgc tctctttcgg gattactgat gaaactttca
      181 cggtagcttc aatgcacaag gagaagatta ctttttccta tatgctgggt ctgatcaccg
      241 gaccttattg ggggtgggct ctcgggaccc tgctcggtgc catgatttgt tcggtacttc
      301 ctgagactct gcaaaattcg atgggtattg ctttgtatgc catgtttatc gcgctggttg
      361 tgcccaatgc caaaaagtcc agagcggctc tggctgtggt tttcatcgcc gtcgccatca
      421 gttccgcctt tacttgggtg ccgtacctgc acggtatttc tcaggggtgg gggattattt
      481 tggcaaccat tgcggcctgt ctggtcgggg caatcttttt ccccagggag gaggagtgaa
      541 gatggctaag gtcctaatcg cggtgttgtt gatggcggtt gttacttata ttatcagggt
      601 cttgccgctg gcttttttca ggagtcaatt aagatccaga tttctccgtt cgtttttata
      661 ttatgttcct tatgctgttt tgggggcaat gacctttccc gccattctct attccaccgg
      721 taatctctat tcggcttgga tcgggatggg cgtcgccctg gtcatggctt tctttgagag
      781 tggattatta agggtggccc tcggcgccat tgtctcggtt tttttagccg aatgggtcat
      841 ccgtctcata tcgttttagg tttcatgccg tttccatcat gacaataatc tatttcaatg
      901 cgatgattaa ggctccggct acgatgagga ccccgccgga taaggtttta aaatcggcct
      961 tctcacccag aaagatgaaa gcgagcagca tcgcgaagac aacccccagt ttatcgatgg
     1021 gggccacccg tgaggccggg ccgagctgta atgcacggta ataacagagc caagaggctc
     1081 cggtggccag ggcggagaga atcaggaacg ttaaggtccg gcccggtagt ttggccgggt
     1141 tttgccattc tcccgtcgca aagacgatgc tacccgagaa gatcagcacc acaatggtgc
     1201 ggagaaaggt agccagattg gaattgatcc cggtaactcc gattttaccg aataaagcgg
     1261 tgagtccggc aaagaaggcc gaaccgagtg cgaaatagac ccagttggga aacatcacct
     1321 atcacatcct tggattttat tgaatatcgg taagaccact aacgatacca tacctacccc
     1381 tctctttctc gtccaccgga gggagggagg tcggcaaggt gagtcgctaa agattccatt
     1441 ccgacgatgc aggttccaac ccaagcaagt cctaccgacc atccggccag cacatcactg
     1501 ggataatgca cacccaggta tacacggctg attccgatta gtaccggtag caataccagt
     1561 ccgataccta taacccggtt taagccgggt cgacggctgt tgcgaaagac cagataagcc
     1621 aagaagccat ataaggccat ggagattaag gagtgaccgc ttggaaaact atacccgttt
     1681 gccgaagtga gccagggtaa atcgggcctg tggcgatgga aggagatttt gagcagttca
     1741 ctgaggagta aagctccacc taccgacaga tttaagacaa gtacctccag acgtctccgg
     1801 atacgaaaac cgatcccgag caggaccaaa agtaaaccga tgatccaacc ggtggatccc
     1861 aggttggtaa tcccccgcat cagccaggtg agccaggcag tacggaaagc gatgatcacg
     1921 gccctgattg ttgtatcaaa atgggttaat tcccgttcca acagttccga ggcgagtttg
     1981 gtgaagatca tgaatccgaa tccggttaag caggccccgg tgatcaggta ccatccgaac
     2041 ataccggcgg gcagatagtt ccggcgtaac ctttctgccc atgaaagcca ttttttgacc
     2101 aagttacgca tgaacctcag aaaaattcca tttttgagtc cggttttcca aaatagaaag
     2161 atgattgtca acgatattaa caaccagatt agatgcaccg ggctaaatgt cgtctgcagc
     2221 aatttggtga tcccccgacc gtaccggatc agtaagattg cctcaataaa aaagcgaatc
     2281 cccctagcaa agatggaagc cggaataaaa ccggccagtc gggtccggaa aacaccggcg
     2341 gcaatggcga agattttgta aggaattggc gtaagccctc cgatgactgt agcccaattc
     2401 ccatagcggt ggtaaaaccc ttccacccgt ttgagacgga cctcgtccac gaaccggata
     2461 agaagcggcc ggcccaatcg aacaccgagg ggatagccga atagtccccc cagtaccgaa
     2521 cccagagtgg cgatgaaggc gtatt
//
LOCUS       DASVMB010000236         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_471365,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000236 DASVMB010000000
VERSION     DASVMB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471365"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VF531_06895"
     CDS             <1..390
                     /locus_tag="VF531_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713733.1"
                     /translation="NEFKKIRDQQHQRYKSDLSAEKFYTAAIKQIKELKPYEKAVFKM
                     AMTVLFGFEGSEEVREKLLEWERSRIIDNRKILDQYVKDASIQEDFAALFVRVTENMI
                     EKIIFLDEDLVDEVIERQLSLLFRLVR"
     gene            518..1297
                     /locus_tag="VF531_06900"
     CDS             518..1297
                     /locus_tag="VF531_06900"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7713734.1"
                     /translation="MHTTIFYFTGTGNSLWVSRYLASKLGDTRLVSLPLSEEVDLSSS
                     RRVGFVFPVIMFGLPLIIKRFVESVPLSRSTYIFAIATCGGKPFNTMKRLEQLIGARG
                     LELSAGFNVEMVNSCTSIGEAIPVVKQQQQFERAKEALNRICLKIEEQERHIDKGIPG
                     LNWYFGDVIHAKAEPQIPGAAQYFYFDKNCNGCGLCEKVCPVKNIGMDSMRPVWHNRC
                     EQCYACLQWCPKEAVQVKNKKTEGLRRYHHPEITPADIIRK"
     gene            1675..>2343
                     /locus_tag="VF531_06905"
     CDS             1675..>2343
                     /locus_tag="VF531_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7713735.1"
                     /translation="MKKINIGGGAFDASEISLGCMRIAGLSRQEASVLINTAIEEGID
                     FFDHADIYADGKAEETFSQVTKMNPGLREKIFIQTKCGIRKGYFDFSKEHILESVDGS
                     LKRLQTDYLDVLLLHRPDTLMEPEEVAEAFTILHDSGKVRYFGVSNQNPMQIELLSKY
                     LKQKLIINQLQLSITNTGMIDSGLNVNMKIDRSIDRDGSILEYCRLKDITIQAWSPFQ
                     FGFFE"
BASE COUNT          699 a          449 c          562 g          633 t
ORIGIN      
        1 aatgaattca agaaaatccg ggatcaacaa catcaaagat ataagtccga cctctctgcc
       61 gagaaatttt atactgcggc aattaagcag ataaaggagt taaaacccta tgaaaaagcg
      121 gtattcaaaa tggcaatgac ggttttgttc ggctttgagg ggagtgagga agtccgggaa
      181 aaactccttg aatgggaaag aagcaggatc atcgataatc ggaaaatcct tgatcaatat
      241 gtcaaggatg cctcgattca agaggatttt gccgcgctat ttgtcagggt tacggagaat
      301 atgatcgaaa aaatcatatt tctggatgaa gaccttgtgg atgaagtgat tgaaaggcaa
      361 ctctcattat tatttcggct agtgagataa atttttttgt ggcttgtata actaacgtta
      421 gatatacaaa tattcaatat ttggtttaag gggttcatac cttaacagtc tatatttgag
      481 tccgatgttt aaaatcaatt atttaaagga ggataatatg catacaacca tcttttattt
      541 taccggaacc ggaaattcat tatgggtttc acgctatctt gcttcaaagc tgggggatac
      601 caggctggta tcgttgccgc tctcagaaga ggttgatctt tcatcaagcc ggcgagtcgg
      661 atttgtattt ccggtgatta tgtttggttt gccactcatc atcaaacgtt ttgttgaatc
      721 ggtcccactt tcccgaagta cctatatttt cgctatcgcc acttgcggag gcaagccgtt
      781 taatacgatg aagcggcttg aacaacttat cggcgcccgt gggttagaac tctctgccgg
      841 attcaatgtc gagatggtaa atagttgcac ttcaattggt gaagccatac cagttgttaa
      901 gcaacaacaa cagtttgaac gggccaagga ggccttaaac cggatttgtt tgaaaatcga
      961 agaacaagag cggcacattg ataagggaat tcctggccta aattggtatt ttggggatgt
     1021 cattcatgca aaagcggagc cccagattcc gggtgcggcg cagtatttct acttcgataa
     1081 gaattgtaac ggttgcggtt tatgtgaaaa agtatgtcca gtcaagaata ttggcatgga
     1141 ttcaatgaga ccggtatggc acaaccgttg tgagcagtgc tacgcctgct tgcaatggtg
     1201 cccaaaggaa gccgttcaag taaaaaacaa aaaaacagaa gggcttagac gctatcatca
     1261 tccggaaata acgccagccg atattataag aaaatgacgt tagattctta attttcttga
     1321 gtgaaattaa tgaaagaggg tcgctcgtaa gcgccccttt ttttgataaa tacagccgga
     1381 tggaacaccc caaacatggg cttgaggctg ccggagcagg taacgcccgg cgataaaaat
     1441 tttggatttc ggacttgtcg ccgcttaccg ccatggatat ataccacaac aggaatcctg
     1501 aaagtatgat gggatagggg tttataaatg cattctatat tgttgataag tttgtgacaa
     1561 aatacaccaa tagtagtgct gatacgggaa aggaaagtta caataaagct agaagatcat
     1621 tatgtagtaa tcatatggaa agcattgatc gatcaatgaa agaggtggtt tttgatgaag
     1681 aagattaaca tcgggggcgg cgcgttcgac gcttcggaga tttcattggg ctgtatgaga
     1741 attgccgggc tttcgcgtca ggaagcctcg gtcctgatca ataccgctat cgaagagggc
     1801 attgatttct ttgatcatgc cgatatttat gccgacggaa aggcggagga aacattttca
     1861 caggttacca agatgaatcc gggtctgcgt gaaaaaatat tcatccaaac caaatgcgga
     1921 atccgaaagg gatatttcga cttttccaag gagcatattc tggagtccgt agatggcagc
     1981 ttaaagcggt tgcaaacgga ttatctcgat gttctgttgt tgcaccgtcc cgatacgctg
     2041 atggagccgg aagaggttgc cgaagccttt actatcctcc atgacagcgg taaggtcaga
     2101 tactttggag taagcaatca aaatccgatg cagattgagt tgctcagtaa atatctaaaa
     2161 caaaagctga tcatcaacca gttacagcta agcatcacca acaccggaat gatcgattcg
     2221 ggcctcaacg tcaacatgaa aatcgaccgt tccattgacc gcgacggcag catcttggaa
     2281 tattgccgct taaaggatat taccatccag gcttggtcgc ccttccagtt cggtttcttt
     2341 gag
//
LOCUS       DASVMB010000237         6729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_692618,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000237 DASVMB010000000
VERSION     DASVMB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692618"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1482)
                     /gene="nagE"
                     /locus_tag="VF531_06910"
     CDS             complement(70..1482)
                     /gene="nagE"
                     /locus_tag="VF531_06910"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HEX7713736.1"
                     /translation="MSKHGFMGNLQRLGKALMTPVAVLPAAALLLRLGADDVFKLKWM
                     SAAGDAIFGNLALIFAIGIAIGFADENNGVAGLAATVGYFVMTKVAGTFNAAINMGVL
                     AGIIAGLLAGFLYNKYKAIKLPDFLGFFGGKRFVPIVTSFWCLLIGIAAGYIWPLIQD
                     AINSFGTFIATSGAIGGFIFGCINRLLIPFGLHHVVNTFVWFQFGQFTTEAGKVVTGD
                     LSRFFAGDPTAGTFMTGFFPIMMFALPAACLAMIAAAKKENRKSVAGMLLGVAFTSFL
                     TGITEPIEFLFMFLAPLLYILHALFTGIALAITAVLGIKCGFGFSAGLIDYVLNYGIS
                     QKPLEIIFIGLAFGVVYYFVFLYIIKKLNLPTPGRMEDEESAALLGLSNTDLRDRAAE
                     ILKAIGGKENISVVDACVTRVRVTVKEATLVDEAKLKAMGATAVLKMPGNNFQIVVGT
                     VADPLVTHMKAIMSNKSISV"
     gene            complement(1661..2515)
                     /locus_tag="VF531_06915"
     CDS             complement(1661..2515)
                     /locus_tag="VF531_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEX7713737.1"
                     /translation="MPEDFEVIKVFNNNVLLAKQLTTEKILIKKGLGFGKKPGERIPQ
                     DTRFDQIFVIENREAQHKFNQLLAEVEESMFGVCEEVLWMIGNEVGEPISEEFHIGLI
                     DHIAFTLYRIQRNDKIENPFIVEIETLYAREYEIAQKAVAILAKNLKVEIPESEIGFI
                     ALHIHSLRNNGKLSNTIKYAYICNSALELIEDELQIEVDRKSIDYARFVCHIRFAVER
                     IIKGIPIKNELLASIRKTYKNSYQLAQQVAAMIEKEIYQKVPEAEIGFITMHVERIKN
                     VSTMLTNG"
     gene            2814..4517
                     /gene="ptsP"
                     /locus_tag="VF531_06920"
     CDS             2814..4517
                     /gene="ptsP"
                     /locus_tag="VF531_06920"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX7713738.1"
                     /translation="MIKGIPASSGVAIGKARIWVKAGWEIEKRTISNVAQEKKRFTRA
                     VATAGEQLEAVKVKVAKEIGQDQAAIFEAQMMMLNDPELLQAVETMIQEQKVNAEYAL
                     RVAMDQYINLLEQVEDEYLRARTADLKDVANRVTGILMDAVDDKGLDQQAIIVARDLM
                     PSETAQLKRDHVLGIVTAIGGTTSHTAIMARTLGIPAVVGADEALESIRNGDLLIVDG
                     DAGLVFINPDQASQERYLAVQEKNLQAGQELLKLRDMEAITRDGHLVELAANIGSVAD
                     AGLVLENGAMGIGLFRTEFLYMDKTNWPTEEEQYDAYREALVKMGGKPVIIRTLDVGG
                     DKKLPYADFPEELNPFLGYRALRLCLDCPDIFKTQLRALYRASVSGKLRVMLPMVASV
                     EELREVKRIAGEVRDQLKNEKISFREDVELGVMIEIPSAAIISAELAQEADFFSIGSN
                     DLIQYTLAVDRTNQRISPLYNPFHPAVMRLIKMVADNAHFAGKPVGLCGEMAADLRLT
                     PLLIGLGLDELSMNPGSLLKVKQRIRNLDSQKCKIMAEQALHLATAAEVQDLITKSSV
                     E"
     gene            4665..4928
                     /locus_tag="VF531_06925"
     CDS             4665..4928
                     /locus_tag="VF531_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7713739.1"
                     /translation="MKQIELIVKSETGLHARPASVVVSKVTHFKSNITVRKGDKEANL
                     KSLLGVLGLGVCQNDSITILIDGPDEEQALKSIVQLGKQFGLW"
     gene            complement(5036..5845)
                     /locus_tag="VF531_06930"
     CDS             complement(5036..5845)
                     /locus_tag="VF531_06930"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEX7713740.1"
                     /translation="MYHFIMDVFNGYRSRLDDVLLVNEVLEEIPLKLGLKTVMPPFIL
                     PYYNGVVPEDCGISAFIFLAGGHFTLHTFSYREAYFVDMLSPEPIDVARLEQLIKEAF
                     PAGRVTSYLLEREKPSTYPQKIEINKALDFGPHLFLDFDHYDGPRTMDDLFELFDTMP
                     YRIGMTPIIRPYAMKNTVDGEKVTSILTMIAESHISLHYFEESQKAYLDLFSCRFFDT
                     GEVIPTLKQIFGSHVNNEVLMARGSKYQKFKTDTNVRAHKSRIWLDNVYKR"
     regulatory      6083..6264
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6398..>6729
                     /locus_tag="VF531_06935"
     CDS             6398..>6729
                     /locus_tag="VF531_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7713741.1"
                     /translation="MGEKIRVLVSGACGRMGREVVKTVLKDETLQLVGAVDKSNPGKD
                     IGTIIGGETLGIQVGEDLGVTIRAVRPQVMVDFTIPRTVMINLRTALGAGIHVVVGTT
                     GITPENLQ"
BASE COUNT         1756 a         1538 c         1795 g         1640 t
ORIGIN      
        1 aaaaggtaca ggagcccgaa ggctcctgta cgggtctgcc tttattccgg aaaatccttg
       61 gttcggcagt tagacgctta tactcttgtt gctcataatc gctttcatat gggtgaccag
      121 cggatcggcc acggttccga caacgatctg gaagttgttg cccggcatct tcagaaccgc
      181 cgtggcgccc atggccttta atttggcctc atctaccaga gtggcctctt ttacggtaac
      241 ccggacccgg gtgacacagg catccaccac cgagatattc tccttgccgc cgatagcctt
      301 taaaatttct gcggcgcgat cccggaggtc ggtgttgctt aatccgagta aagcagcgga
      361 ttcttcatct tccatccgac ccggagtggg caggttgagc tttttgatga tgtagaggaa
      421 gacgaagtag tagaccacac caaacgccaa tccgataaag atgatttcca gcggtttctg
      481 ggagatgcca tagttcaaaa catagtcgat caacccggcg gagaaaccga aaccgcactt
      541 gatccccagg acggcggtga tcgccagggc gataccggtg aacagcgcat gcagaatgta
      601 gagtagcgga gctaagaaca tgaagaggaa ttcaatcggt tcggtgatac cggtcaggaa
      661 cgaggtgaaa gccacgccca ggagcatacc ggcgacgctt ttccggtttt ccttctttgc
      721 cgcggcgatc atcgccaggc aggcggcggg aagagcgaac atcatgatcg ggaagaagcc
      781 ggtcatgaag gttccggctg tgggatcacc ggcgaagaaa cgggagagat ccccggtaac
      841 cactttaccg gcttcggtcg tgaactgacc gaactggaac caaacaaagg tattgactac
      901 atggtgcaga ccgaagggga ttaacaggcg gttgatgcaa ccgaagatga aaccgccgat
      961 ggccccggag gtcgcgataa aggtaccaaa ggaattgata gcgtcctgaa tcaatggcca
     1021 gatgtaaccg gcggcaatac cgatgagtag gcaccaaaac gaagtgacga tgggcacgaa
     1081 gcgtttgccg ccaaagaacc cgaggaaatc cggaagcttg atcgctttgt atttgttata
     1141 aaggaacccg gccaacaaac cggcgatgat accggccaaa acacccatgt taatcgcggc
     1201 attgaatgta ccggccactt tcgtcatgac gaagtagccg acggtagcgg ccagtcccgc
     1261 cacaccgttg ttttcatcgg cgaagccgat ggcgatgcca atggcgaaga tcaacgccag
     1321 attgccgaaa atggcatcac cggcagcgct catccatttc aacttgaaga catcgtccgc
     1381 tccgagccgt aacagcagtg ccgctgccgg aagcaccgct accggggtca tcagagcttt
     1441 acccaacctt tgcaggttgc ccataaaacc gtgcttggac ataagatcac cctcaccctt
     1501 cttttttatt gccaacctga agaattcagg ttagtcacat gatcaataaa gcctaaaaaa
     1561 taagaaaaga gctgaaaaga ttaactttcc agctcttgcc gatagttgca cgccagtaaa
     1621 aaaaaatacg tattcaattc atcttcattt tagggtatcg ttaaccgttt gtcaacatcg
     1681 ttgaaacatt ttttatccgt tcgacatgca tggtgatgaa accaatctcc gcctcgggaa
     1741 ctttttgata tatttctttt tcaatcattg ccgcgacttg ttgggccagt tgataagagt
     1801 ttttatacgt cttacggatc gaggccagta gttcgttttt gatggggatg cctttgatga
     1861 tccgttctac cgcgaaccgg atatgacaga caaaccgggc gtaatcgatg gatttccggt
     1921 cgacctcgat ctgcagttca tcctcgatca actccagggc ggagttacat atataagcgt
     1981 acttaatggt gttggagagc ttcccgttat tacgcaatga gtgaatatgg agggcgataa
     2041 agccgatttc actctcgggg atttcgacct tcaaattctt ggcgaggata gccacggctt
     2101 tttgagcgat ttcgtattcc cgggcatata gcgtctcgat ctccacgatg aaagggttct
     2161 caatcttgtc gtttctttga attcgataca gcgtaaacgc gatatggtca atgaggccga
     2221 tatggaactc ctcggagatg ggttctccga cttcattccc gatcatccag agcacttcct
     2281 cgcatacccc gaacatactc tcctcgacct cggccaagag ttgattgaac ttatgttgtg
     2341 cctcgcggtt ctcaatgacg aagatctggt cgaaacgggt atcctgaggg atgcgctcgc
     2401 ccggcttttt cccgaagccc aatccctttt tgatcagtat tttctcggtg gtaagctgtt
     2461 ttgccaataa aacgttattg ttaaaaacct tgattacttc gaaatcttcc ggcaaaacta
     2521 tacccccttt gtcgattgcg gttggttcta ttataacaca cagcagtcgt gaattttttt
     2581 tggaaacgtg tgaatatttg gatggcgcgg tttttccgat atagaattaa tccttgtgaa
     2641 actcgacccg gaaattgatc ttgccaagtg ctgcaagatt aggtaaaata aaattaatgc
     2701 aatgaatgat cgatttaaag gcatccaggt gtgttactga attcaggcat taacctgtga
     2761 atcccttgcg ggattttagg ttaatgcctg atttttttgg tgaggaggat accatgataa
     2821 agggaattcc ggcatcatca ggggttgcaa tcggaaaagc ccggatatgg gtcaaggccg
     2881 ggtgggagat tgagaaacgg accatctcaa acgtggctca agaaaaaaag cgattcacga
     2941 gggcagtggc tacagccgga gaacaattgg aagcggttaa agtcaaggtg gctaaggaga
     3001 tcggtcaaga tcaggcggcg atcttcgagg cgcagatgat gatgttgaat gatcccgaac
     3061 tcctgcaggc agtggagacg atgatccagg aacaaaaggt gaatgcggag tatgcgctgc
     3121 gggtagccat ggaccagtat atcaacttgc ttgaacaggt tgaggatgag tatcttcggg
     3181 ccaggaccgc tgatctcaag gatgtggcca atcgggttac cgggatcctg atggatgcag
     3241 tcgacgataa aggcctcgat caacaggcga tcattgtcgc ccgtgacttg atgccttccg
     3301 agactgccca gttaaagcgg gatcatgtgt tgggaatagt taccgctata ggcggtacga
     3361 cttctcatac ggcgatcatg gcccggactt tgggaattcc cgcagtggtc ggagcggacg
     3421 aagcgctgga gtcgatccgt aacggcgatt tgctaattgt agatggcgac gccggcctgg
     3481 tgtttatcaa tccggatcag gcctctcagg aacggtacct ggccgtacaa gaaaaaaatc
     3541 tccaagcagg gcaggaactt ttgaaacttc gggatatgga agcaattacc cgggatggcc
     3601 acctggttga gctggcggcc aatatcggca gtgtagccga tgccggtctg gtcttggaaa
     3661 atggcgccat gggaatcggg ctcttccgga ccgagttttt gtatatggat aagaccaact
     3721 ggcccacgga ggaggagcaa tatgacgcct accgggaagc cttggtcaag atgggcggca
     3781 agccggtgat catccggacc ctggatgtcg gaggggataa gaaactgcct tacgccgact
     3841 ttcctgaaga actcaatccg tttctcggct accgcgccct aagactatgc ctggactgtc
     3901 cggatatctt taaaacacaa ttacgggccc tctaccgcgc ctcggtatca ggaaagctcc
     3961 gggtgatgtt gccgatggtg gccagtgtcg aggagttgcg ggaggttaag cgtatcgccg
     4021 gggaagtcag ggatcaattg aagaatgaga agatttcttt ccgggaagac gtggagttgg
     4081 gagtaatgat tgagattccc tcggcggcga ttatctcggc ggagctggcc caggaagccg
     4141 attttttcag tatcggcagt aatgatctaa tccagtatac cctggcagtg gatcggacca
     4201 accaacgaat ctctccctta tataatccat ttcatcccgc ggtgatgcgg ttgatcaaga
     4261 tggtggcgga caatgcccat ttcgccggaa aaccggtggg actttgcggt gagatggcgg
     4321 cagacctgcg gctaacgccg ttattaatcg gtttgggcct ggatgagttg agcatgaacc
     4381 ccggctcgct gctgaaagtt aaacaacgga tccgcaacct ggatagtcaa aaatgcaaga
     4441 ttatggccga gcaggcgctc catctggcga cagcagccga ggttcaggat ttgatcacca
     4501 aatcatccgt tgaataacga atacttaatt gcttccattg aatgtatttt catgtgcgtt
     4561 taatcgaaaa gcaacttagt ataccaaacc gatttgtctc ttttggttca atggatgaat
     4621 gcaaattggg aaaaaacgca aaatcgggaa ggggggcggg gttcatgaaa cagatcgaac
     4681 tgattgtaaa gagtgaaacg ggccttcacg ccagaccggc ttcggtggtt gtgagcaagg
     4741 taactcattt taaaagcaac atcacggtcc gtaagggaga taaagaggcc aatctaaaaa
     4801 gcctcttggg tgtcttgggc ctgggagtct gccaaaacga ttcgatcaca attcttatcg
     4861 atggtcccga tgaagagcag gctttaaaga gcatcgttca actcggcaaa cagtttgggc
     4921 tttggtgagt gcggtttgga atagatttat ttcagtcgct tcggatcaac caaaatacaa
     4981 ccaaagaaat aacccgggag ggattcccgg gttatttctt aaccttttat caagtttatc
     5041 gcttataaac attgtcaagc cagatacggg atttgtgtgc ccggacattg gtgtccgtct
     5101 tgaacttctg gtacttgcta ccccgcgcca ttaaaacttc attgttgaca tggcttccga
     5161 atatctgttt cagagttgga atgacctccc cggtgtcaaa gaaacggcag gagaagaggt
     5221 ccagatatgc tttttgggac tcttcgaaat aatggagact gatatgactc tcggcaatca
     5281 ttgttaaaat ggatgtcacc ttttcaccat ccaccgtatt cttcatggca tacgggcgga
     5341 tgatcggagt cataccgatc cggtagggca tggtgtcaaa tagttcaaag agatcatcca
     5401 tggttctcgg accatcgtaa tgatcgaaat ccaagaagag atgaggcccg aaatccaagg
     5461 ctttattaat ctcgatcttc tgcgggtagg tggaaggttt ctcccgttct aaaagatacg
     5521 aagtgacccg gcccgccgga aatgcctcct tgatcaactg ctctaaccgg gcaacgtcga
     5581 tcggttccgg agaaagcatg tcgacaaagt aagcctcccg gtaagaaaag gtatgcaatg
     5641 taaaatgtcc gcccgcgagg aatatgaagg cactaatccc gcaatcttcc ggaactacac
     5701 cgttataata cggcaggatg aacgggggca tcaccgtttt cagccctaat ttcaagggga
     5761 tctcttccaa tacttcgttc acgagcagta cgtcatccag acgggaacgg tagccgttaa
     5821 agacatccat gatgaaatga tacatcgctc atcgcctctt ctgatttaaa ctggacttct
     5881 attatattaa aacaagaacc ggttgaagga tatagtttta acaaaactga aatagccgaa
     5941 ttttgatgat accatcttaa cccgcaattt gcaaggcctc tttacggact gacgttttgc
     6001 ctgcctcctg gttttacagg ccataggctt gacagccgag gcgttttata gtaaaaatgg
     6061 atcaatatat taagagtttt cgtggtagag tagaggtgcg gaacgcatca gtagctccgg
     6121 ggaggccggc aggccgggga agcggagtca aagggcgttt cgccgaagcc ggggaaagcc
     6181 gtttttccga ctggtatcgc cgtaaagaac tgcgatactg tcatccgttg tttaaaatga
     6241 cacggatgaa gcgctatctt gccatcttat gaattacgga ttccggagtt cagggttggt
     6301 ggatgccaac cctttttgta tgggtttcgg ttttacggaa cagactttgg atgagccggg
     6361 ttgcaaaact ttgctataaa ttaaggagtg gtgatgtatg ggggaaaaga tccgagtttt
     6421 agtttccgga gcatgcggcc gtatgggccg ggaagtggta aaaaccgtat taaaggatga
     6481 aaccttgcaa ttagtgggag cggtagataa aagtaatccc gggaaggata tcggcaccat
     6541 aatcggggga gaaacattgg gaatccaggt cggtgaggat ctgggcgtaa cgattcgtgc
     6601 cgttcggcct caggtgatgg tggatttcac cattccccgg acagttatga tcaacctgcg
     6661 gaccgctctg ggtgcgggca ttcacgtagt agtcggcact accggaatta ccccggaaaa
     6721 tctccagga
//
LOCUS       DASVMB010000238         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_396448,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000238 DASVMB010000000
VERSION     DASVMB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396448"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VF531_06940"
     CDS             complement(<1..722)
                     /locus_tag="VF531_06940"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7713742.1"
                     /translation="MRKGIWAIFVVLMVLMLIGGQATRTVYAEASNEHRPNVYDALDK
                     LTANEVAELQASITNVSKEYNLDVAVVITDQTQGKSSRDFADDFYDNQGYGVGPDHSG
                     LLLLINMDQREVWISTAGKAIDIFTDDRISNMVNDVTAPLSNGNFDEACKTFVDDVGS
                     IAKMGVPAGQNRVGSETDEAVSEDQDETDTQPVAGSSYFGRMFGMMKSVWVYVIALIV
                     ALIATFLVSRSSKGKVTINNLT"
     gene            complement(715..1746)
                     /locus_tag="VF531_06945"
     CDS             complement(715..1746)
                     /locus_tag="VF531_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7713743.1"
                     /translation="MDTVKAYKCPNCKAGLEFNPANQNWKCQYCFGEFSKEQLDAVLQ
                     PEEPLEQAPTELNSYRCSSCGAELIADATTSATFCLYCKNHTIIKSRFAGEFKPERLI
                     PFKLTKEQAKELYRKWITKRLFAPDDFKQKDKLDKVTGVYAPFWLFDCKVDGMIEGEG
                     TRVRTWSDADYKYTETKYYRVVRGGSTEYNRVPVDASIKLDDQFMHLIEPYNYGDLTE
                     FSMEYLSGFMAEKYDVEAAAAETVMAERVSQYLEDRLTATVSGYSSFTVSNKLIQLKD
                     TIHEYAMLPIYLLINQYQGKDHVFIVNGQTGKVVGDTPTSFAKQLLFAGAVFFGVWLV
                     LVLGGALFA"
     gene            complement(1762..3138)
                     /locus_tag="VF531_06950"
     CDS             complement(1762..3138)
                     /locus_tag="VF531_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7713744.1"
                     /translation="MGIIKAAIGAAGGGLADQWLETIEPAEMDNTTLATYGVKVRKND
                     SRSSNKKGTEDIISNGSIIHVPVNTYMLLVDGGKIISATEEEGYYQVDNSTSPSIFFQ
                     ASDKVETAGYGNTGQNAIKRPGGLVNTLKDSWERFKFGGTSPLKQTVVYINKQEIPDI
                     RFGTKTPVPYTDRVLVPGRVVPCKVTSFGTYSIKIGDPLLFYSEVYSKTNNVSLSTMD
                     LAEQYINEFLMAYTTALASLSTNNVLVSDIPIKTLELGQYMAEVLDKEWLSKRGFYIH
                     SVGIAGISFDEKTNELLDKYANISIMMDPNARAARMTEGMAAGLQAAGSNSGGAMVGF
                     AGMAMGMNAAGAMGLMPGQQLANGPGNPAASTVAGGWVCSCGNTMAAESAFCSKCGSK
                     KPASLSPAGGDVWQCSCGRSVSEGVFCPSCGKRKPEDKKWTCSCGNVTEDTFCSKCGN
                     KRPDASTD"
     gene            complement(3473..3943)
                     /locus_tag="VF531_06955"
     CDS             complement(3473..3943)
                     /locus_tag="VF531_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HEX7713745.1"
                     /translation="MGIMSGIKQALGVGGVKVSLTVPEQVEKSKGQVEGNLALTAKSD
                     QEILKLTVTITESYTTGRGDEETTKEYDLGEVNLPAPFQMKAGEAKEVPFVLPFKLLK
                     SDADVLKEKGGALGMLGKASAFANAEKSTYKVKAEADVKGTMIDPSDEKDIKLV"
     gene            4726..>4982
                     /locus_tag="VF531_06960"
     CDS             4726..>4982
                     /locus_tag="VF531_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713746.1"
                     /translation="MGENQLAIYPLTSERWSDFERLFGKDGAHGGCWCMWWRIPEDQF
                     ERQCGETNKNAMQEIVNSGKVTGIIAYIDDQPVGWCSLGPR"
BASE COUNT         1291 a         1228 c         1093 g         1370 t
ORIGIN      
        1 taagtcaggt tattgatggt taccttgccc ttactggagc gggaaacgag aaatgtggca
       61 attaacgcga ctatcaaggc aatcacataa acccatacgg acttcatcat cccgaacatt
      121 ctgccaaagt atgaactacc ggcaactggt tgagtatcgg tttcatcctg gtcctcggat
      181 acggcttcat ccgtttctga acccactctg ttctgtcctg cgggtacacc catcttggca
      241 atagaaccga catcatcgac aaaagtttta caagcctcat caaaattgcc gtttgaaagg
      301 ggagcggtaa catcattgac catattcgaa attcgatcgt cggtaaaaat atcaatggct
      361 ttaccggcgg tagatatcca aacttctctc tgatccatgt tgattaacaa caaaaggccg
      421 gagtgatcag gacccacacc atatccctga ttatcataaa aatcatcggc aaagtccctc
      481 gatgacttac cctgggtttg gtcggtaata accacagcca catccagatt gtattctttg
      541 gagacattcg tgattgaagc ttgtaactcc gccacttcat tcgctgtcaa tttgtccaga
      601 gcatcataaa cattggggcg atgctcgtta gaggcttcag cgtatacggt gcgggttgcc
      661 tgtcctccga tcagcatcaa caccatcaaa acgacaaata tagcccatat ccctttacgc
      721 aaacaaagca ccccccaaca ccaatactaa ccatacgccg aaaaacacag ccccggcaaa
      781 taaaagctgc ttggcaaagc tggtaggagt atcgccaacc actttgccgg tttgtccatt
      841 gactataaat acatgatctt tgccttgata ttggttaatc agcaaataaa tcggcaacat
      901 ggcgtattca tggatggtat ccttcaactg aatcaattta ttactgacag taaaagatga
      961 ataacccgat acagtggcgg tcagtctgtc ttccaggtat tgagataccc tttctgccat
     1021 cacagtctcg gctgcggctg cctcaacatc gtatttttca gccataaacc ccgataaata
     1081 ttccatggaa aattcggtga ggtcgccgta gttatatggt tcaatcaaat gcatgaactg
     1141 gtcgtccagt tttatggatg catccaccgg cacccggttg tattcggtac tacccccgcg
     1201 gactacccgg taatacttag tttcagtgta tttgtaatcg gcatcgctcc aggtacggac
     1261 cctggttcct tctccttcaa tcatcccatc aactttgcaa tcaaacagcc agaagggagc
     1321 gtaaactccg gttaccttat caagcttgtc tttttgttta aaatcatccg gagcaaacaa
     1381 acgcttggtg atccactttc gatacagctc tttagcctgc tccttcgtaa gcttgaatgg
     1441 aatcaagcgc tcaggtttga attctccggc aaagcgcgac ttaatgatgg tatggttctt
     1501 gcaatacaag caaaaggtag ccgaagtggt agcatcggca atcagttccg ccccacaact
     1561 gctacaccgg taggagttca gttccgtcgg agcctgttcc aaaggttctt ccggctgcaa
     1621 taccgcatca agttgttctt tactaaactc accaaaacaa tattgacatt tccaattttg
     1681 attcgcgggg ttgaactcca atccagcctt acaattggga catttatacg cttttactgt
     1741 atccattgtt acgctcctta ttcaatctgt agaagcatcg ggtcgcttgt ttccacactt
     1801 agagcagaac gtatcctcgg ttacattacc gcaagaacaa gtccatttct tatcctctgg
     1861 tttccttttg ccgcaactcg ggcaaaacac tccctccgaa acgcttctgc cacaggaaca
     1921 ttgccacaca tcgccacctg ctggggatag actagccggt tttttggaac cgcatttgga
     1981 acagaaagcg gattccgcgg ccatggtatt gccacaggaa cagacccagc cgccggctac
     2041 cgtcgacgct gccgggtttc ccggcccatt ggccaattgc tgaccgggca tgagacccat
     2101 tgctcctgcc gcattcatcc ccatggccat tccggcaaac ccaaccatgg ccccgccaga
     2161 atttgaaccc gcagcttgca accccgccgc cataccctct gtcatccggg ccgcccgggc
     2221 gttgggatcc atcataatgg agatgttggc gtatttatcc agcagttcat tggtcttttc
     2281 atcaaaggaa attccggcaa tccctacgct atgaatataa aacccccgtt tggacagcca
     2341 ctctttgtcc aaaacttccg ccatatactg tcccagttct agggtcttaa ttggaatatc
     2401 cgaaaccagc acattgttgg tagagaggct ggccaaggcc gtggtatagg ccatcagaaa
     2461 ttcattaata tattgctcag ccagatccat tgtgctgaga ctcacattgt tggtcttgct
     2521 ataaacctcg ctataaaaca ataacgggtc gccaatctta atcgaatagg ttccaaagga
     2581 ggtaacctta caagggacta ctcttcccgg caccaacacc ctatcggtat agggaaccgg
     2641 agtcttcgtg ccgaaccgga tatcgggaat ttcctgttta ttgatataga ctacggtttg
     2701 tttcagcggt gacgttccgc caaatttgaa ccgctcccag gagtccttca gtgtattgac
     2761 taaaccgccc ggtcgcttga tggcattctg tccggtatta ccgtaacccg cagtctcaac
     2821 tttatcggaa gcctgaaaga agatggaagg cgacgtggaa ttatccactt gataataccc
     2881 ctcttcttca gtggcggaga tgattttacc tccatctacc agcaacatat aggtgttcac
     2941 cggcacatgg atgattgagc catttgaaat aatgtcctcg gtgccctttt tgtttgagct
     3001 gcgggagtca tttttcctga cttttacccc gtaggttgcc aaagttgtgt tgtccatttc
     3061 cgccggttca atagtttcca gccattgatc ggccaacccg cccccagcag cgccaatcgc
     3121 cgctttgata atacccatac gtcaatcatc ctttcaaatc agcttttttt caagaatgtt
     3181 aataatccag ttgttcaatc tcaaccaacc aataaatggt tcaagtaaag cataatatat
     3241 tggttatttc ggtgttttcc atcaaattcc tgtaaaaaaa ctgatcttat ttgtgggaat
     3301 ttcaataaaa ttgtccttct ttattcatgg aggcacgaca ccgtttatca tctcacgact
     3361 cagcgacaac cggcggaaaa gttggagccg cggggaggaa accggcgcga accggatccc
     3421 tcatgcgcgg ctcctacctt ccggcctgtc tcgctcgagc ggccgtccaa gctcagacga
     3481 gcttgatgtc cttctcgtcg ctgggatcga tcatggtgcc cttgacgtcg gcctccgcct
     3541 tcaccttgta ggtcgacttc tccgcgttgg caaaggcgct ggccttgccg agcatgccga
     3601 gggctccgcc cttttccttg agtacgtcgg cgtccgactt gagaagcttg aaggggagca
     3661 cgaagggcac ctctttcgcc tcgccggcct tcatctggaa gggggctggg aggttgacct
     3721 cgccgagatc atactccttg gtggtctcct cgtcaccgcg ccccgtagtg tagctctcgg
     3781 taatggtgac ggtgagcttg agtatttcct gatcactctt ggcggtgagg gccaaattcc
     3841 cctcgacttg gcctttgctc ttttccacct gttcgggcac ggtgaggctg accttcacgc
     3901 ctccgacacc caacgcctgc ttgataccgc tcataatacc cataaaacga ttctcctttc
     3961 ttcaggacgg ctcgcgggct ttaccgccgg tttgaaatcg caccgagtcc tgaaggtttt
     4021 tattttttat tacatgtttt aattttaaca gaattttaat tattggacaa gcttttatgc
     4081 caaattgtgt taaattataa tattatgata tgtaaatcat tggagagata atttgatgca
     4141 agctacagca gcttaagata taaaagaatt gaacagcgaa catagaatgt tattttttta
     4201 gtctagagaa aggagttttt ttccagcaag agaatatctt tcttgtatcc agatcatttg
     4261 ggctccgatt ggcggatttt aatgaatttt tgtattgcat ttaggtttaa atctgcaaca
     4321 attacttcga aagcctccac ttctactttt tacaactttg ttttgagact ttccaacttg
     4381 aagatgaagg agttattaaa ttcatcggtc ttaaaaattt aaaaaaacaa gggacttgtg
     4441 tttcaaagac gttgagaatt atgattgtcg tatttggtca taaaaaagag acattatgtt
     4501 gtagaaaagc acaagatttc cgcacatatg acatatgtac atataacacc acgttactgc
     4561 ttgtggggcg cgagattcga agaagttgtt cgatgaagaa agtgtatcat gaggttatat
     4621 caaggagaaa attcatgaag taggtgagct atctaaggcg gcaggacttc tgtaactcgt
     4681 agatgttagt acgccaagcg gagggaaaat gagaggagta atcttatggg tgagaaccag
     4741 ttagccattt atcctttgac atcggaaagg tggtccgatt tcgaaaggct ttttggaaaa
     4801 gacggtgcgc acggcggttg ctggtgcatg tggtggcgga taccggaaga ccaatttgaa
     4861 cggcaatgtg gagaaacaaa caaaaatgca atgcaggaaa ttgtcaattc ggggaaggta
     4921 actggtatca ttgcatatat tgatgaccag cctgttggat ggtgctcact tggcccgaga
     4981 ga
//
LOCUS       DASVMB010000239        10184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_471651,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000239 DASVMB010000000
VERSION     DASVMB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471651"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1142)
                     /gene="pdxA"
                     /locus_tag="VF531_06965"
     CDS             complement(114..1142)
                     /gene="pdxA"
                     /locus_tag="VF531_06965"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7713747.1"
                     /translation="MNQKPIIGISMGDPAGIGPEIAVKALAQKEVYQSSNPVIVGDSS
                     VIQAAIRICNLNFTVRPVTAVEAAGFEYGVIDVFDLKNVDMSQLEYGKVSAMAGNAGF
                     TAVKKLIELAMAHKIDATVTGPINKEAMNLAGHHFSGHTEIYAEYTGTKDYTMMLAHE
                     NFRVAHVSTHVSLRQACDRVQKERVLKVIQLSHEAIRKLGIEKPRIGVAGLNPHAGEN
                     GMFGREEIEEITPAIEAAKGQGILVEGPIPPDSLFPKGKSGFYDVVVAMYHDQGHIPL
                     KLAGFNWNEAAKRWESVNGINITLGLPIIRSSVDHGTAFDIAGKGIATPESLLSAIDY
                     AQKMARLS"
     gene            complement(1182..2426)
                     /locus_tag="VF531_06970"
     CDS             complement(1182..2426)
                     /locus_tag="VF531_06970"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX7713748.1"
                     /translation="MDILIIADDLTGANDTNVQFAKFGARAITCYDLRSLALYPGFDI
                     ASLSTESRSLTPENAYQKAATVIRRLPFQPEYFVYKKIDSALRGSFGIEIDALMDNMP
                     WIKATLVAPAYPDNHRTTINGSQLVKGVPVHQTEIAADPVTPVTDSNLLTVLGSQSRR
                     KIGYLPLAQVLKGYPAVQSYIHSLISDHCEIIVADAETNEHLSTLARVIWDEKSLIPC
                     GSAGLALALAEIYRQRSDWQQTKSINPSPFLAVVGSKSDVAGLQVTTARQELPNIRML
                     AIDTDNLVDSSRAGAQMDRAVTAALRIFAQERFLVLYLSSKVMHQSTDAPIIASGLAE
                     IACRILQQVKIKTLFATGGDTAAHIFDKLGIQALELENELEPGICKGRFVGGTYDGLG
                     VITKAGSFGDAHTLVRIIKNIH"
     gene            complement(2477..3616)
                     /locus_tag="VF531_06975"
     CDS             complement(2477..3616)
                     /locus_tag="VF531_06975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7713749.1"
                     /translation="MKSFQLISQFPQTVFYGAGTAEKVEEILGDPRGKKVLIVTDSFL
                     VKSSMIETFSNSLTDKPAIYGEITGEPTSEEVEKAVRFCREGRFDLVIGIGGGSVLDT
                     AKVVAALGRDQIKVSQVYGAGKLKKREIGLALIPTTAGTGSEATPNSLIFDSVTGNKE
                     AIIGRELIPDWVVLDPVLTTGLPPKVTAATGLDALCHCIESYYSVNSNPVSEAYSYEG
                     LKLLGANLGRAVEEPDNLEVRGDMLLGSFFGGLGLTIAGTTAVHALSYPLGKRRVAHG
                     VANGMLLPKVLEYYFPHIEGKLNQVFRLFATHEKAVHNSRELLDMIAGYVSQFPIPKT
                     LAEVNISADEIPQLAKEAVRNERLIRNNPGPVTIDDAEKIYRMII"
     gene            complement(3635..4564)
                     /locus_tag="VF531_06980"
     CDS             complement(3635..4564)
                     /locus_tag="VF531_06980"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7713750.1"
                     /translation="MYTEEQKKKFKGIGALVLTIFDQDLKLNTNALQKNIRFMIDNHI
                     NASNGFLVCNGSTGECYAMNLEERKQVIQATVEAANKEIPVVACCNDTDAFSVIELAN
                     HAKKVGADAALIIQSYYLPFNEEQIYNFFQFVNDRVDIPIMIYNNPSVCSGVEMSMDL
                     LKKLAKLNKVFALKQTTNSSKHFIQSQVLAPELLIFTGSSSTEPFGDMAGMHGFFSFL
                     SCFYPQMQIELWEAIRTKDYVAAGKIHTKELLLYDWWWSGGLGQTFGQIVHAKKALDL
                     MGHYGGYSRPPLVPLKEAQTEKLKQILQAWGLI"
     gene            complement(4598..5440)
                     /locus_tag="VF531_06985"
     CDS             complement(4598..5440)
                     /locus_tag="VF531_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7713751.1"
                     /translation="MAKGSKKSLKFRIIFYVSLVLLVLFSIGPIIWMLFSSLRPKNEF
                     FMIPPTIFPKHWTVINYFLIFKDTAFFQFLLNSIWISAGTIVISTVVGILAAYSLTRF
                     NYKGRNLFALFSLFAYMLPTILLVIPLYLLVVQIGLADKLTGLVLAYVALCLPYCIWL
                     LRSFFQTIPIELEESAFIDGAGRIKTLLHVVLPMMIPGIVATTVYSFTYVWNEYLFAL
                     IFMTSDAKRTFPVGLESFVTSFDVYWEYILSGSIIVSIPAVVVFLATQRSLIKGYGAG
                     AVKG"
     gene            complement(5433..6332)
                     /locus_tag="VF531_06990"
     CDS             complement(5433..6332)
                     /locus_tag="VF531_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7713752.1"
                     /translation="MQLTKKDNQIGMAFLIPALIFMAILIIYPLVNSFILSFYRQLIY
                     EAKGVYIGFQNYTKILSDPEFWHSLQLSVIWTVLTIAGQLIIGIGSALILNEEFRGRG
                     LMRAFVLLPFFMPTVAATLMWRWLLNEQYGAVNYILMSMHIIDKPISWLANPSLAMLT
                     VIFIGIWRYFPFVTINVLARLQTIQQELYEAASVDGANAWHKFCHITLPEIKGVLIVV
                     ILLRSLFMFNKVDIILLLTGGGPGNNTLTLPVQAYSYAFEGMQIGRGAANSILQFLII
                     LVFIIIYMKSTSEGQKKGGKIYG"
     gene            complement(6426..7727)
                     /locus_tag="VF531_06995"
     CDS             complement(6426..7727)
                     /locus_tag="VF531_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7713753.1"
                     /translation="MKRLLLVGLLISLFVIAGQSFAASQTITYLTPETDPSSIAVDNH
                     IIAAFEKAHPGVKVNLTHASLNDVLPKLSAMLKAGTAPDVAFSSPKFVPPLVDQGYLA
                     PMDDVFKKLGDISPKFVAANSKGKIYDVPAAVEARMLYYRKDLFEAAGLKPPTTFDEW
                     YKAAKALTVDKNGDGKIDQYGISLTGAPPECSYDICIIVWANGGDLFDKNNRVVLDNP
                     KAVEALEFLGKMKEFTPPGMATTTYKDVSLNFANGVAAMAVYPGRMMMNIDRYNPELR
                     DKVGVVPIPYGPGGNNKPVVHESLNDFIVFKTSKNVKLAKEFVSFYMSGKQYLEFLTA
                     STPGHSLPVRNNWLDDPDYFKFEAIARWKDNVKTSMDSAFKYGTDFLFRYPGVVNPYV
                     GRATADPTFAREVNKYFTGESTAKQALSNVANTWREMFGIK"
     gene            complement(8009..8692)
                     /locus_tag="VF531_07000"
     CDS             complement(8009..8692)
                     /locus_tag="VF531_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7713754.1"
                     /translation="MAFKPMKTNLLHMDVVNAIISAIVTGELKPGDRIIEQHIAEQMQ
                     ISRAPVREAIRELVAQGIVRFLPRKGAYIVPLEPDEIRNVYLLRSRLEGLAAFIATPK
                     LLNNDLNMLLSFSENMETATKENDAKKFIDNDLKFHDLICQTCGNENLQKMIAGVRIQ
                     IQLFMMMSKWNLVIHSQLTKESRTHQPIIQAFLKSDADLAEQCMRNHVISAGAMLIEY
                     VEENKSNIS"
     gene            complement(8956..9183)
                     /locus_tag="VF531_07005"
     CDS             complement(8956..9183)
                     /locus_tag="VF531_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713755.1"
                     /translation="MRGEDDKVRFIDFKNGPRKRVDEKRTGTDLAKQINGRLTYLNLT
                     AARLDESEDPLDNLLANVMFDCLPGNRIQRK"
     gene            9333..10043
                     /locus_tag="VF531_07010"
     CDS             9333..10043
                     /locus_tag="VF531_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713756.1"
                     /translation="MIPIPNQDLLRSYIDQYSLDALFESDMTSHMSLFYYPKGELICE
                     GSAKLHYMFFLVAGKLKTFTMLDNGKSLLLRFSRPLSVLGDLEFLSRFPVRCNVEAQM
                     DSYLIGIKFDDLYQYAYNDPTFLRFLINNLSFKLYTVSNSATLNQLYPLENRFASYLL
                     SVSVYVCEAENGESRVEEIKTPKLTEIAMMLGTSYRHLNRVIGQFRRDGIISQKKGSL
                     TVLDLSRLKILAKENIYE"
     gene            10074..>10184
                     /locus_tag="VF531_07015"
     CDS             10074..>10184
                     /locus_tag="VF531_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA-like transporter family protein"
                     /protein_id="HEX7713757.1"
                     /translation="MQGILFSLIGGILICLQGVFTSRISSKIGLWETNTLV"
BASE COUNT         2820 a         2404 c         2087 g         2873 t
ORIGIN      
        1 aaattcgtta taatgtgaaa aaggcctctc ccaaaaggaa aggccttttc aatttctagg
       61 agaaatcggg gattaagcgc aagcgcaaca agtcgcggca gggtttcctt gggttacgat
      121 aaacgggcca ttttttgggc atagtcaatc gcgctaagaa ggctttccgg agttgcaatc
      181 ccttttccgg cgatatcaaa agcggttcca tgatctactg agctgcggat aatcggcaag
      241 cccaaggtaa tattgattcc attgacggat tcccatcttt ttgcggcctc attccaattg
      301 aaccctgcca atttcagggg aatatgtcct tgatcgtggt acatggccac caccacatca
      361 taaaacccgc tcttcccttt gggaaacagg ctatccggag gaatggggcc ttcaaccaga
      421 atcccttggc ccttggccgc ttcgatggcc ggcgttatct cttcaatctc ttccctgccg
      481 aacatcccgt tttctccggc atgaggattg agaccggcca ccccaatccg gggtttttcg
      541 attcccaatt tgcggatcgc ctcgtgggaa agctgtatga ctttcaacac ccgttccttt
      601 tggacacggt cacaagcttg ccttaaagaa acatgagttg aaacgtgagc gactctaaag
      661 ttttcatggg ccagcatcat cgtataatct ttggtgcccg tatactcggc ataaatctcg
      721 gtatgacctg aaaaatgatg accggccaga ttcatcgctt ctttattaat cggaccggta
      781 acagtggcat ctattttgtg ggccatggca agttctatca atttctttac ggccgtaaat
      841 ccggcatttc cagccatagc cgatactttc ccatattcga gttgcgacat atccacattt
      901 ttaagatcaa aaacatcaat tacgccatat tcaaatccgg cagcttccac cgctgtaacc
      961 ggtcggactg taaaattcaa gttacaaatg cgaattgcgg cttgaataac actgctgtcc
     1021 cccacaataa ccgggttaga ggattgataa acttcttttt gggcaagtgc ttttacggct
     1081 atttccggtc cgattcccgc cggatcaccc attgatattc cgatgatggg tttttgattg
     1141 atcatggctc tttatccccc gtttgttctt gcggattttc cctaatggat atttttaatg
     1201 attcgtacta atgtatgtgc atctccaaat gaaccggctt ttgtaataac tcccaaacca
     1261 tcatacgttc cgccaacgaa ccggcctttg caaatgcccg gttcaagttc gttttccaat
     1321 tcaagagctt ggatccccaa tttgtcgaag atatgagcag ctgtatcccc accagtggca
     1381 aacagggtct ttattttgac ctgttggagt atacgacagg caatttccgc caaaccggag
     1441 gcaatgatcg gcgcatcggt actctgatgc ataacttttg aagataaata tagtactaaa
     1501 aatcgttctt gggcaaatat tcttagcgcc gcagtaactg ctcgatccat ttgagccccg
     1561 gctctcgaag agtccaccaa gttatcggta tcaatcgcca acattctgat atttggaagt
     1621 tcctgccggg cagtcgtcac ctgtaaaccg gcaacatcgc ttttactccc aacaaccgcc
     1681 aaaaaagggc ttgggttgat tgacttagtt tgttgccaat cgcttcgctg tcgataaatt
     1741 tcggccaggg ccagggccag cccggccgag ccgcacggga tcaaactctt ttcatcccaa
     1801 ataacccggg ctaaggttga gagatgttca tttgtttcag catcagccac aataatttca
     1861 caatgatcag aaatcaagct atgaatatag gattgaacgg ctggatatcc tttcaacact
     1921 tgggccaaag gaagatatcc tattttacgg cgactttggg agcctaacac cgtcaataaa
     1981 ttcgaatcgg ttaccggtgt caccggatcg gcggctattt cagtttgatg aaccggaact
     2041 cccttcacta actgagaacc gttgatagtt gtacgatgat tgtcaggata agccggggca
     2101 accagtgttg cttttatcca cggcatattg tccatcaaag cgtcgatttc aatcccaaaa
     2161 gaacccctta acgcggaatc gattttttta taaacaaaat attccggttg aaacggtaat
     2221 ctcctgatca ctgtagccgc cttttgatag gcgttttccg gtgtcaaact cctgctttcc
     2281 gtggaaagac tggcaatatc aaaacccgga tataaagcga gggatctcag atcataacaa
     2341 gtgatggccc tggcgccgaa cttcgcaaat tgtacatttg tatcattggc accggttaaa
     2401 tcatccgcaa taatcaagat atccaatttt acggcccccc atccgtcaga gatacctcac
     2461 gaaaatagtc tttcacctaa atgatcatgc ggtaaatctt ttcagcatca tcgattgtaa
     2521 ccgggccggg attatttctg attaagcgtt cattgcgaac cgcctctttg gccagttgcg
     2581 gtatttcatc cgccgaaata ttaacttcgg caagtgtttt cggtatgggg aattgcgaca
     2641 catacccggc aatcatatcc agcaattcac ggctattatg cactgctttt tcatgagttg
     2701 cgaataaacg aaagacctga ttcagtttac cttcaatatg tgggaaataa tactccagca
     2761 ccttcggcaa gagcatcccg ttggcaaccc catgagccac tctcctttta cccaaaggat
     2821 atgacagggc atgaactgcg gttgtcccgg caatggtcaa ccccaagcct ccaaaaaagc
     2881 ttcctaagag catgtctccc cgtacttcca ggttatctgg ctcttcaacg gctctgccga
     2941 gattggcgcc gagcaatttc agtccctcat aagaataagc ctcactgacc gggttgctgt
     3001 ttaccgaata ataagattca atgcaatggc aaagggcatc cagtccggtg gcagctgtta
     3061 cttttggcgg aagtcccgtg gtcaggactg gatccagtac aacccaatcc gggatcaact
     3121 cccggccgat aatagcctct ttattgccgg tgaccgagtc gaaaatcaac gaattcgggg
     3181 tagcttccga accagtcccc gccgtggtcg gaatgagggc caaaccaatc tcacgctttt
     3241 ttagctttcc ggcgccatac acctggctca ctttgatttg gtctctcccc agcgcagcga
     3301 caactttggc ggtatccagc acactaccgc ctccaatgcc tatcaccaag tcaaaccgcc
     3361 cttcacggca aaaacgaact gctttttcaa cctcttccga agtcggttct ccggttatct
     3421 ctccgtatat cgcaggttta tcagttaaag aattggagaa ggtttcgatc attgacgatt
     3481 tcactaaaaa actatcggtg acgattaaaa ccttttttcc cctgggatcg cccagaattt
     3541 cttcaacttt ttcagcagtc ccggcaccat aaaacacagt ctgtggaaac tggctgatca
     3601 attgaaagga tttcacagct gacatctcct tatgttaaat taagccccaa gcttgtaata
     3661 tttgtttcaa tttttcagtt tgggcttctt ttaagggtac cagaggaggt ctggaatacc
     3721 cgccgtaatg gcccattaaa tccaaggcct ttttggcatg aacaatctgg ccaaacgtct
     3781 ggccaagccc gccactccac caccagtcat acagcaataa ttccttggta tgtatcttcc
     3841 cggcggcaac ataatccttt gttcgaatgg cttcccacaa ttcgatttgc atttggggat
     3901 aaaaacacga taggaaggaa aagaaaccat gcatcccggc catatctcca aaaggctctg
     3961 tcgaagagga acctgtaaaa attaatagtt cgggtgccag aacctggctt tgaataaagt
     4021 gtttcgaaga gttcgtggtt tgttttaagg caaacacctt attcagttta gccagttttt
     4081 ttaacaaatc catcgacatc tccactccac tgcaaaccga agggttgtta tagatcatga
     4141 ttggaatatc aacccgatca ttcacaaatt gaaagaaatt atagatttgt tcctcgttaa
     4201 aagggagata ataggattga ataataagtg ccgcatcggc gccgaccttt ttggcgtgat
     4261 ttgccaactc gattacagag aaggcatccg tatcattaca acaggcaacc accggaatct
     4321 ctttattggc agcctcaaca gtagcctgga ttacttgctt ccgctcctcc aaattcatag
     4381 catagcattc tccggtactg ccattgcata ccagaaatcc attcgatgca ttgatgtgat
     4441 tgtcgatcat aaagcgaata ttcttttgca gtgcattcgt atttaatttt aaatcttgat
     4501 cgaagatggt taatactaat gcaccgatgc ctttaaactt ctttttttgc tcctcagtat
     4561 acattgttcc tcctaaataa ttgtcatcga ggatgcgtca gcctttgact gcaccggctc
     4621 cataaccttt gatcagcgac cgctgtgtgg cgaggaagac aacaaccgcc ggaatactta
     4681 cgataatact gccgctcaag atatattccc aataaacatc aaaggatgta acaaaactct
     4741 ccaatccaac cggaaaagtc cttttcgcat cgctggtcat aaagatcaag gcgaataaat
     4801 actcattcca cacataggta aaactataaa ccgtcgtggc tacaattccc ggaatcatca
     4861 tcggtaatac gacatgaagc aaagttttaa ttcgtccggc tccatcaata aaagcggact
     4921 cttccaattc gatcggtatg gtttgaaaga aagagcgcaa cagccaaatg caatagggaa
     4981 gacataaagc cacataagcc aggactaaac cggttaattt atcggccagg cctatttgta
     5041 caaccagtaa atacagggga attaccaaaa ggatggttgg caacatataa gcaaaaagag
     5101 aaaataaggc aaataaattt cttcccttgt aattaaaacg ggtcaaacta taggcagcca
     5161 gtatacctac aactgtgcta ataacaatcg tccctgccga gatccaaata ctgtttaata
     5221 aaaactggaa aaaggcagtg tccttgaata tcaggaaata attgattacc gtccaatgtt
     5281 ttggaaaaat cgtcggtgga atcatgaaaa attcgttttt gggacgaagg gaggaaaaca
     5341 gcatccagat aatcggtccg atcgaaaata gtaccaacaa aaccaatgaa acatagaaaa
     5401 taatccgaaa tttaaggctt ttcttggaac ccttagccat agattttccc tccctttttc
     5461 tgcccttcgc tggtggattt catataaata atgatgaata ccagaatgat caggaattgc
     5521 aatatggaat tggctgcgcc tctaccaatt tgcatgcctt caaaagcata actatacgct
     5581 tgtaccggca aagtcaatgt gttgttaccc gggcctccgc ctgtcagcaa tagaataata
     5641 tcgactttat taaacataaa gagactccgc agcaggataa caacgataag tactcctttg
     5701 atctccggta gggtgatatg gcaaaattta tgccaggcat tggcaccatc gacactggcc
     5761 gcttcataca attcttgttg gattgtctgc agtctggcca gaacattgat tgttacaaag
     5821 gggaaataac gccaaattcc gataaagatc acggttaaca tcgcaaggga aggattcgcc
     5881 agccaactaa tgggtttatc aatgatatgc atggacatta atatataatt taccgccccg
     5941 tactgttcgt tcaataacca tctccacatc agggtggctg caacagtggg cataaaaaag
     6001 ggtagcagga caaaagccct catgaggcct ctacccctga attcttcatt gagtattaac
     6061 gcgctgccga ttccgattat cagctgcccg gcaattgtca gaaccgtcca aattacactc
     6121 agctgcaacg aatgccaaaa ctcaggatcg gataaaattt tcgtataatt ttgaaaaccg
     6181 atgtatacgc ctttcgcttc ataaatcagc tgccggtaaa agcttaagat aaatgaatta
     6241 acgagcggat agatgattaa tatggccatg aagattaagg caggtatcaa aaaagccatt
     6301 ccgatctggt tgtctttttt ggtgagttgc atttcgacct ccaaatttct cgaaggctga
     6361 ttattccgta gaataatcag ccttgtactt aattgttttc gttggttccc gctatgaagt
     6421 cgatattact taataccgaa catttcacgc caagtattgg caacattgga caaggcctgt
     6481 ttggcagtac tctctccggt aaagtattta ttgacttccc gtgcaaaggt aggatccgcg
     6541 gtagctctac caacataagg attcacaaca ccgggataac ggaacaggaa gtcggtgcca
     6601 tatttgaatg cactatccat ggaggtcttt acattgtctt tccatcttgc gatggcttca
     6661 aatttgaaat aatccggatc atcaagccag ttgttccgga ccggtaaact atgtccggga
     6721 gtggaagcgg ttaggaactc cagatactgc ttgccgctca tataaaagct gacgaattcc
     6781 ttggccaatt tcacgttttt ggaggtcttg aaaacgataa aatcattcaa ggattcatga
     6841 acgaccggtt tattattgcc accgggtcca taaggaatcg gaaccacacc gactttgtca
     6901 cgaagctccg ggttataacg gtcgatgttc atcatcatcc ggccgggata tactgccatt
     6961 gccgccacac cattggcaaa atttaacgaa acatccttat aggtggtcgt ggccatcccc
     7021 ggaggagtga attccttcat tttacctaag aactccaatg cctcaaccgc tttgggatta
     7081 tcaagcacga ctctgttgtt tttgtcaaat aaatctccgc cgttggccca aacaatgata
     7141 caaatatcat aactgcattc cggaggagca ccggtaaggg aaataccata ttgatcgatt
     7201 ttgccgtcgc cgtttttgtc aacggtcaag gccttcgcag ctttatacca ttcatcaaaa
     7261 gtcgtcggag gttttaaacc ggcggcttca aaaaggtctt tccggtaata aagcattctg
     7321 gcctcaacag cagccggtac atcataaatt ttccctttgc tgttggcagc cacaaatttg
     7381 ggggagatgt ctcccaattt cttgaacaca tcatccatcg gagcaagata gccttgatcc
     7441 acgagaggcg gaacgaactt cggcgaacta aaagccacat ccggagctgt tcctgccttt
     7501 agcattgccg agagtttcgg taagacatcg tttaagctgg catgggttaa attgaccttc
     7561 accccggggt gggctttttc aaacgctgca ataatgtgat tgtccaccgc aatagaagac
     7621 gggtcggttt ccggggttaa ataggtgatt gtctgagatg cagcaaagct ctgcccggcg
     7681 ataacgaaaa gtgaaatcag taaaccaaca agcaacaatc ttttcattac aatttccccc
     7741 tttgtttttt tatttcttac cggtaattcc cgcgatttaa acttccatca cctccttttc
     7801 tgttccaatg ttttatgtcg attgtcgaca ataaacagca aaaagaatta gacctttccc
     7861 acatatatcg aagcgatctt cttccacttc gaatcatctc tacttctctg atgcatctat
     7921 cgaatgattc ccgctgattt attttcggtt ctctatatct ccgcttaatg tcgattgtcg
     7981 acaataagca gacaaaaata ctgccagctc aggatatatt agacttattt tcttccacgt
     8041 attcgattaa catagcacct gcagaaataa catggttccg catgcattgc tcggcaagat
     8101 cggcgtcgga cttaagaaag gcctgaatga taggctgatg ggtcctgctt tctttggtta
     8161 attgggaatg gatgaccaga ttccatttgg acatcatcat aaataattga atctgaatcc
     8221 ttactccggc aatcattttt tgtaagtttt catttccgca agtttgacag atcaaatcat
     8281 gaaatttgag atcattatcg ataaattttt tggcgtcatt ttctttggtt gctgtctcca
     8341 tgttttccga aaaacttagc agcatattta agtcgttatt caatagtttg ggcgtagcga
     8401 tgaaagcagc gagtccctcc aaccggctcc ttaacagata tacgtttcgt atttcatccg
     8461 gttcgagtgg aacaatataa gcgcctttcc gcggcaaaaa ccttactata ccttgagcca
     8521 ccaactcgcg aatggcttcg cgtaccggtg cgcggctgat ttgcatctgc tcggcaatat
     8581 gctgttcaat gattcgatcg cccggtttta gctcaccggt aacaattgcg gaaatgattg
     8641 cattaactac gtccatatgc aataaattgg ttttcatcgg tttaaatgcc atcttcgatc
     8701 tcctatgtcg attgtcgaca atatacttat tcaatcttta tttacaaatt ccttttttta
     8761 aatctgatga ttaatattat attcgttacc aaaaagcact aatgggaaca cccttaaaca
     8821 tgtgctttgc gcgcatgctc gcgcaactac caacccacct cgggacttgc atccgttgga
     8881 ttgagcccct gccggcgcag tgcaaaaaag gcctctccca aaaggaaaga cctttttata
     8941 tcaagctaca atcatttatt ttctttgtat ccggttcccc ggaaggcagt cgaacattac
     9001 gttggccagg agattatcca aaggatcttc cgattcatcc agacgagcgg cagttaaatt
     9061 taggtatgtc aaccggccgt tgatttgctt ggctagatcc gtcccggttc ttttttcatc
     9121 aacccttttt cgcggtccat ttttaaaatc aatgaatcgc actttgtcat cctcaccccg
     9181 catctacctc acctcatcat ctccgcctta cttccaatat tgaccaataa cctaggactc
     9241 atacattttt gtgcatcatg acatttgtcc ttttccaatt gttgcttttt atatatactt
     9301 aatttaagca agttcaagga gaaggcggaa atatgatacc gattccgaac caggatttat
     9361 tgcgttccta cattgaccaa tattcgttgg atgcgttatt tgaatcggat atgaccagcc
     9421 acatgagctt gttttattac ccgaaaggcg aacttatctg tgaaggttca gctaaactgc
     9481 attatatgtt ttttctggtg gccggaaaac taaagacctt taccatgctg gataacggta
     9541 agtcactttt attgcggttt agcaggccgt taagtgtgct tggcgatttg gaatttttaa
     9601 gccgcttccc ggtaaggtgt aacgttgaag cacaaatgga ttcctatttg attggaatta
     9661 aatttgacga tttgtatcag tacgcctata acgatccgac ttttctgcgg tttttaatta
     9721 acaacctaag ttttaaactt tatacggtct cgaattcggc aaccctcaac cagctttacc
     9781 ctttggaaaa ccgttttgcc agttacttat tgtcggtgag cgtatatgta tgtgaagctg
     9841 agaacggcga gtcgcgggta gaagagatca aaaccccgaa attgacagag atcgcgatga
     9901 tgcttggaac aagctaccgg catctaaacc gggtaatcgg gcaatttcgg cgggacggga
     9961 ttatttcaca gaaaaagggg tcgctgacag tcttggatct ttcgcggttg aaaatcctgg
    10021 caaaggagaa tatttacgaa tagacaacaa catttatcgg aggaggtaag tacatgcagg
    10081 gaattttatt ttcacttatc ggtggaatcc ttatttgtct ccaaggagtc ttcacttcca
    10141 gaatcagctc caaaatcggt ttatgggaga ccaataccct cgtc
//
LOCUS       DASVMB010000240         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_692689,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000240 DASVMB010000000
VERSION     DASVMB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692689"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VF531_07020"
     CDS             <1..1016
                     /locus_tag="VF531_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7713758.1"
                     /translation="LENLADGILVLDKRLDLILVNPAARRMLGLDPKTAEGRPVMEAV
                     LNHHLLDLIQQVNRTQEPLQSELPLYYPTDAQLQVLLAPLKDETGSIQGTIIVLHDLT
                     QLRRLERVRQDFVANVSHELRTPVAAVKAMTETLLSGGWHESQMLLRYLRAIDQESDR
                     MTNLINDLLALARLDSKTEVVRQPFDLVDLIAEVRERFTPVTPDAPVLKLSLPETPLP
                     LAYGNRDQIKQVLINLLDNAFKYTPTGGSIRIAIRAEAEMLKVMVADTGIGIPEADRD
                     RIFERFYRVDKGRSRELGGTGLGLSIVKHIVESHGGKVTVESKLNRGSVFSFTLPVAI
                     GKG"
     gene            1264..2421
                     /locus_tag="VF531_07025"
     CDS             1264..2421
                     /locus_tag="VF531_07025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7713759.1"
                     /translation="MRSKSMALVIAICLVMQSFWAPIEGAFSINARAAVLMDPATGKV
                     LYAQNENQRLPPASVTKVMTMLLILEAVHAKRVTWDETITASATAAGMGGSQIYLKEG
                     ETMPLRDMFKAIAVVSANDASAAVAEHLYGSLEDFIDAMNEKAKTLGLKNTHFANETG
                     LPDPSHYSSAYDLAVISRHLLQYPEVLKFTSIWLDSLRSGKFILRNTNELLKAYRGAD
                     GLKTGHTAEAKFCLSATAVKGGFRLLSVILGADTDAVRVSQSKRLLDYGYRTFERRLV
                     QKAGSDVGLITLPEASPAKIPVRLRTDFAVVVERGKEQLITTRLVPDKKLRLPVTAGQ
                     RVGSLQAEVSGVSVGSAPVYTTKAAHRANFLVRWWRMFADWVSGFFRRGKR"
     gene            complement(2429..3673)
                     /locus_tag="VF531_07030"
     CDS             complement(2429..3673)
                     /locus_tag="VF531_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7713760.1"
                     /translation="MSSLKLFLLLFLIGKLVMRLGLNLLNYLHLKHTAGQVPAALTGV
                     VEPSKLQQIDAYNAAKMIFQMVYILWDNLILLLFLFTPAYPLYSSWIDSLTLPLFLRG
                     LLFFGIIAAGSWLIGLPFACFFHFRIEEKFGFNRYTLWRWFGDELKNLLIDLIVLTII
                     FWIVLAYWGNDLSFQWGRVWGAWLIALGLIVLFMYLVPVLVIPFFYRLKPVTDEGLRQ
                     KIIHLVENCGLKVSKVVEADESQKSAHANAQMAGIGRTKTIILFDTLIANYNESEILA
                     VLAHEIGHGKKRHLWIQIALAAVEFFLLIYFTARLLSSSATFTAWDTSQVIYNGVFIA
                     FIIFGEIMSFFVTPFSSYLSRKLEYEADRFSKQVLQSGAPLRGVFLKFIVNELDNINP
                     HPWYEAFYYSHPSLLKRIKALE"
     gene            3839..4000
                     /locus_tag="VF531_07035"
     CDS             3839..4000
                     /locus_tag="VF531_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713761.1"
                     /translation="MEYIAIFIAALVALYTILFALENWKEKNRSGAIAIILLALVELG
                     LPFYLLVFK"
     gene            complement(4009..5310)
                     /locus_tag="VF531_07040"
     CDS             complement(4009..5310)
                     /locus_tag="VF531_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7713762.1"
                     /translation="MMRRRLSIILILLWLSAFLGGCWDRRELESLGLVQALGIDRGPG
                     GKGITVSTMIAIPPKISSGGGQSGGSGGGGGTDSGVFIISMDAPTIYEAFNRINTVVN
                     REITLMQTSALFIGEDLARSGLHKWVDTFVRYREMRRTILVFICKGTAAQMMKVQPKL
                     EKNPSEFFTDLSGLTSRTGMFPKTRLHDFMFSYEAYAQEEYAPILASYQPKEPVAPKP
                     KPGGGGQSGGQGSSGGGQSGGGSPGSPGQGQGPSHIRIIGTAIFKKDKMIGTLDNYES
                     QVMLLLSGRFHEAMLSIADPKHKKELIAFRLFASGAPQIIYERRKNQDRLQVRLKLEA
                     DILSIQSGVNYTDPSKERYLSKYIAAALKRRIEKVLDKVQHQYQSDVFGFGNRVRASF
                     LTSSEWERYNWPKHFPQAQIHTQVNLSIRRVGVQFAPPTLR"
BASE COUNT         1315 a         1343 c         1408 g         1275 t
ORIGIN      
        1 tcctggagaa tctggccgac ggcattttgg ttctggataa acggttggac ctgatattag
       61 tcaatcccgc ggcccggcga atgttaggac tggatcccaa aaccgccgaa ggccggccgg
      121 taatggaagc ggtccttaac catcatttgt tggacctgat tcagcaggtc aaccggaccc
      181 aggaaccact ccagagcgag ctcccgcttt attaccccac cgatgcccag cttcaagttt
      241 tactggcgcc gctaaaagat gaaaccggat ccattcaagg aacgattatc gtgctccatg
      301 atttgaccca gttacgccgg ttggaacggg tccgccagga cttcgtggcc aatgtctcac
      361 atgaactgcg cacgcccgtc gccgcagtca aggcgatgac agagaccctg ttatccgggg
      421 gatggcacga gagccagatg cttctccgat acttacgcgc cattgatcag gaaagcgacc
      481 ggatgaccaa cctgatcaat gatctattgg ctctggccag gctggactca aaaacagaag
      541 tagtacgcca gccgtttgat ctggttgatc tcatcgccga ggtccgggaa cgtttcacac
      601 cggtgacccc tgatgctccg gttttgaagc tctctctacc ggagactccg cttcctctcg
      661 cttacggaaa tcgggaccag atcaagcaag tcttaatcaa tttgttggat aacgcattca
      721 agtatacccc gaccggcgga agcatccgga tcgcgatccg ggccgaagcg gaaatgctca
      781 aggtgatggt ggcggatacc ggaatcggca ttcccgaagc ggaccgggat cggatctttg
      841 agcggtttta ccgggtggat aagggccgtt ccagggagtt gggcggcacc ggtttgggac
      901 tctccatcgt caagcatatt gtggagagcc acggcggtaa agtcactgtg gaaagcaagc
      961 tcaaccgggg ttcggttttt agctttacct tgccggtggc tatagggaaa gggtgatgag
     1021 ttttattggt cagtcttctt tggttatgct tcgcagttta acccccagcc cttggaccct
     1081 gaatttcaag ccgttcccat tagggaaagt gtgaatagac ttcctccttg taatacttat
     1141 tcccgttttt gggtatcctt tgactatatt gcggcacgaa acgactggga tttgccatac
     1201 cgaaccggtc cggagttttc ccggattata ccggaccgga gccgtctcca aggaggaatc
     1261 tggatgcgat ccaaatcgat ggcgctggtg atagccatat gcttggtcat gcagtctttc
     1321 tgggctccga ttgagggggc cttttcgatt aatgcccggg cggcggtgct gatggatccg
     1381 gcaacaggga aggtattgta tgcccaaaat gagaatcaga ggcttccgcc ggccagcgta
     1441 acgaaagtta tgaccatgtt actcatcctg gaggcggtcc acgccaaacg agtcacttgg
     1501 gatgaaacga ttaccgcttc agcgaccgca gccggaatgg gcggttcgca aatctatctc
     1561 aaggagggcg agaccatgcc gcttcgggat atgttcaaag ccatcgctgt ggtctcggcc
     1621 aacgacgcca gcgccgcggt ggcggaacat ctttacggat cgttggaaga ctttattgac
     1681 gccatgaatg agaaggccaa gaccttgggt ttgaaaaaca cccactttgc caatgaaacc
     1741 ggtttgcccg atccgagcca ttacagcagc gcttatgatc tggcggtgat ctctagacac
     1801 ctgttacaat atcccgaagt gctcaagttc acttccatct ggctggatag tctcagaagc
     1861 ggcaagttta tactacggaa caccaatgaa ctgctcaaag cataccgggg agcggacggc
     1921 ttaaagaccg ggcataccgc ggaagccaag ttctgcctct ccgctacggc ggtaaaaggc
     1981 gggtttcgtt tgttgagtgt gattttgggg gctgataccg atgcggtaag ggtcagccag
     2041 agcaaacgcc ttcttgatta cggttatcgc acttttgagc gccgactggt ccagaaggcc
     2101 gggagtgatg tgggactcat caccctgccc gaagcttccc cggcaaagat tccggtccgg
     2161 ttacgcaccg attttgccgt agtggtggaa cggggtaagg agcagctgat caccacccga
     2221 ttggtccctg ataaaaaact gcgtctgcca gtcacagccg ggcagagggt tggcagtctc
     2281 caggctgaag tcagcggggt ttccgtgggt tcggccccag tctacacgac caaggcggcc
     2341 caccgtgcca atttccttgt ccgttggtgg cgaatgtttg cggattgggt ctccggtttc
     2401 tttagaaggg gaaaacgcta gcgattggtt actccaaagc cttgatccgt ttcaaaaggg
     2461 aggggtggga gtaataaaaa gcttcatacc acgggtgggg attgatatta tccaactcgt
     2521 ttacgataaa tttcaaaaag actcctcgta acggtgcgcc actttgtaag acctgcttgg
     2581 aaaaacggtc ggcctcgtat tcgagtttac gggagagata ggaagaaaaa ggcgtaacaa
     2641 agaagctcat gatctcacca aaaataataa aggcgatgaa gaccccgtta taaattacct
     2701 gacttgtatc ccaggccgta aaggtggcgc ttgaggagag caagcgagcg gtgaagtaaa
     2761 ttaaaaggaa gaattccaca gcggccagcg cgatctgaat ccagaggtga cgctttttcc
     2821 cgtgaccgat ctcatgggcc aacaccgcca ggatctccga ttcattgtaa tttgcgatca
     2881 gtgtgtcaaa gagaatgatc gtcttcgtcc gcccgattcc ggccatttgg gcattggcat
     2941 gagccgattt ttggctttcg tccgcctcca caaccttgct cactttcaag ccgcaattct
     3001 ccaccaaatg aatgattttt tggcgcagcc cttcgtcggt taccggtttc agccggtaaa
     3061 aaaagggaat caccagtacc gggaccagat acataaagag cactatcaaa ccaagtgcga
     3121 tgagccaggc gccccagacc cgtccccatt ggaagctcag atcattgccc caataggcaa
     3181 ggacaatcca gaagataatc gtcaatacga tcaagtcgat caaaaggttt tttaactcat
     3241 ccccgaacca gcgccacaaa gtatagcggt taaaaccgaa cttttcttcg atccggaagt
     3301 gaaagaagca ggcaaaaggt aacccgatca gccatgaacc ggcggcgata atcccgaaga
     3361 aaagtagccc ccggaggaat aacggcagtg taagactgtc aatccacgaa gaatacaggg
     3421 gataagccgg agtaaacaag aataacagca agatgagatt atcccagaga atatagacca
     3481 tttgaaagat catttttgcg gcgttatagg catcaatctg ctgtaatttg gagggttcca
     3541 ccactcctgt caaagcggct ggaacttgac cggcggtatg cttcagatga agatagttta
     3601 atagatttag ccccaatcgc ataacgagct tgccaattaa aaaaagcaac aagaataatt
     3661 tcaaagaact catgaaaacg ccctccgtag gccaggatca tacatgatta attgagttat
     3721 ctatattcga cgcgggtaac ggttaccctg tcgtcaccgg aataattttc tgcaagtaca
     3781 agatgccggt taatcctgga cccggcaaag tataacacgg tcaaaggcgg ggtttggtat
     3841 ggagtatatt gcgatcttca ttgcggcctt ggttgctttg tatacgattt tgttcgccct
     3901 ggaaaactgg aaggaaaaga atcgcagcgg ggctattgct atcatcttat tagccttggt
     3961 cgaattgggt ttgccttttt atttgttggt gtttaaatag ggggtcacct atcgcagtgt
     4021 gggcggagcg aactggaccc ctacccggcg aatggacaga ttgacttggg tatgaatctg
     4081 agcctgcggg aaatgttttg gccaattata acgctcccat tcggatgagg taaggaacga
     4141 cgcgcggacg cgattaccaa aaccaaagac atccgattga tactggtgtt gtactttgtc
     4201 gagaaccttc tcaatccttc gtttcagcgc cgccgcgata tatttggata gataccgttc
     4261 cttcgaggga tcggtataat taacgccact ttgaatactg aggatatccg cctccaattt
     4321 cagtcgaacc tggagccgat cttgattttt acgacgctca taaataatct ggggcgcgcc
     4381 ggaagcaaaa agcctaaagg cgattagctc ctttttatgt ttcgggtcgg cgatgcttaa
     4441 catggcttca tgaaatctgc ccgaaagcag cagcatcacc tgactctcgt agttatccag
     4501 ggttccaatc attttgtcct ttttaaatat tgcagtgccg atgatccgga tatgggacgg
     4561 tccctgaccc tgtccgggag atccgggtga accccctccc gattgtcccc ctccggatga
     4621 accttggccg ccagattgcc cgccgccccc cggttttggc ttgggggcga caggttcctt
     4681 cggttggtaa cttgcgagaa tgggcgcata ttcctcttgg gcataggctt cataactgaa
     4741 cataaaatca tgcagtctgg tcttggggaa catgccggtt cttgatgtga gaccggatag
     4801 atcggtgaag aactcggagg gattcttctc cagttttggt tggaccttca tcatctgggc
     4861 ggctgtgcct ttacaaataa aaacaagaat cgtccggcgc atttcccggt agcggacaaa
     4921 agtatcgacc catttgtgga ggccgcttct cgccagatct tccccaataa acagcgccga
     4981 agtttgcatc agggtgatct cccggttcac cacggtgtta attcggttaa aggcttcata
     5041 tatggtgggt gcatccatgg agatgataaa gactccgctg tccgttcctc ccccaccgcc
     5101 cgatccgccg gattggcctc ctccgctgga tatcttgggc ggaatggcga tcatggtgga
     5161 gacggtgatt cccttcccgc cggggccgcg atcaatccct aacgcctgga ccagccccag
     5221 actttccagt tcgcgccgat cccaacatcc gcccaagaac gcactgagcc aaagcagaat
     5281 caatattatt gacaagcgcc gccgcatcat tcagttgttt gctccttttt attgaagatt
     5341 a
//
LOCUS       DASVMB010000241         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_545678,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000241 DASVMB010000000
VERSION     DASVMB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545678"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VF531_07045"
     CDS             complement(<1..635)
                     /locus_tag="VF531_07045"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX7713763.1"
                     /translation="MKKQLCGVLAVILITALLSLTVYGLGAETSRQYEFKGFTALSVV
                     SGMKLEVTQGEKYEVMIRSNQLELVRVEHSGELLRFYLPPFTVTTASINIFITMPELT
                     KLDLSGGSIAHITMKVTPKNFRASLSGGSRITGTLQAKRLELDLSGGSQTSLTGKADT
                     MVIDASGGSRIQQELFPVKEARLRLSGGCRAEVSVAEVMDIDASGGSQVYYR"
     gene            complement(820..1836)
                     /locus_tag="VF531_07050"
     CDS             complement(820..1836)
                     /locus_tag="VF531_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7713764.1"
                     /translation="MSTIKDVAKLAAVSPSTVSRVIADSQRISPETKARVRKVMKELN
                     YHPNMIARSLITRSSQTLGLILSRSASSAFLNHFFPEIIRGISTITQKRHYNLMLATA
                     EDYSEEAKQAMRMIAEKRVDGIIILATRVNDELIRELTKSDFPFVVVGRIPESIKCHS
                     VNNDNIQASYTAVRHLLNHGHQRIAMINGPEEFTFCQDRFDGYRLALREFGVEYDPAL
                     IRSGALTQEEGYRLTKELLKVQPLPSAVFCIDDLLAVGAYQAIKEARFVIPDSIAVIG
                     FNDDPVAAVMEPHLTTTRIPIYEMGVNAAEIIIQLLEGKEVSPMQKILSSELIIRQSC
                     GTQI"
     gene            2277..4598
                     /locus_tag="VF531_07055"
     CDS             2277..4598
                     /locus_tag="VF531_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HEX7713765.1"
                     /translation="MNQKFISDEWKIIEPGFDPDTNRFSESIFSLGNEHMGLRGFFEE
                     GYSGDSLQGTYIAGIFYPDKTRVGWWKNGYPEFFAKVVNSTNWIAIDLELDGTKLDLA
                     RCRVSDFRRELDMKEGRLTRSFICHSEDGKDTAIVFTRFLSMADNNLACISATIKPLN
                     YSGKIMLAPGLDGNVTNEDSNYGEKFWEEVDKAVAQDSAILLMKTKKSGYIQATAFGV
                     RTFLDGREIFPEKALQQNEEKVGYRMAITVEAGQKLELQKFVAVCTSRDYDVHELRNV
                     TLDKLRLACEKGWASLYREHTEEMARKWEESDVVIEGDPLAQQGIRYNIFQLNQTYAG
                     KDPRLNIGPKGFTGEKYGGSTYWDTEAFCFPFYLYTNQTVARNLLYYRYLHLERAKEN
                     ARKLGLRGALYPMVTMDGQECHNEWEITFEEIHRNGAIAYAVYNYTNYTGDNSYLFQY
                     GIEVLVEISRFWTDRATYNPQKDRYMILGVTGPNEYENNVNNNWYTNTMAAWTLEYTL
                     QACREIEAADPGRYREVVSKSGIAVDEFSKWRDIVTKMYYPFLEESGVFEQNDLYRDK
                     EQVLVKDLDPAELPINKHWSWDRILRSCFIKQADVIQGIYFLPERFDQDCMRRNFDYY
                     EPRTVHESSLSASIHSVVASWIGYKTKAYELYLRTARLDLDNYNDDTDDGVHITSMAG
                     SWAAIVQGFAGALVRDDTLCLNPYVPDQWQGYSFMISFRGRKLKIRVTQEELHIEQLG
                     GEPLLLNVSGRESLSETGKDQVIPIQPRVAVTG"
BASE COUNT         1219 a         1066 c         1232 g         1240 t
ORIGIN      
        1 cgatagtaaa cttgggatcc gccgctggca tcgatatcca ttacttcggc aaccgatact
       61 tcggctctgc acccgccgga gagacgaaga cgggcctctt ttaccggaaa aagttcctgc
      121 tgtatccggc tgccgccgga ggcgtcgatc accatcgtat ccgctttacc ggtaaggcta
      181 gtttggcttc ctccggaaag atcgagctcc agtcgcttcg cttgaagagt acctgtaatc
      241 cttgagcctc cggaaagact ggctctaaag ttttttgggg tgaccttcat ggtaatgtgg
      301 gctatacttc cgccacttaa gtcaagtttt gtcagctccg gcatggtaat aaagatattg
      361 attgacgcgg tggttacggt gaatggtgga agataaaacc gtaataattc gccggaatgc
      421 tccactctga ccagctcgag ttggttactc cggatcatta cctcatattt ttcaccttgg
      481 gttacttcca gtttcatgcc tgagacgaca ctcagcgcgg taaatccttt aaattcatat
      541 tggcgggatg tctcagcgcc gagcccgtaa acagtcaggg ataaaagggc agtgatcaat
      601 atcaccgcta acactccgca taattgcttt ttcatgacct gtttcactcc tcaaatgttc
      661 tccttgatca taaaaacgaa acgacaggtt tttttcattc aaaaaaggct accggtctga
      721 acgataccgg agatgtttat ttataagtaa gaaagtttct cttttggtct gctactttta
      781 aacgaatttt ttgccattta acgggcggat gacttagcct tatatttggg taccacagga
      841 ttgccggatg atcagttccg aggagaggat cttttgcatg ggagagacct ctttcccttc
      901 caggagctga atgatgatct ctgcggcatt gacacccatt tcataaatag ggatccgggt
      961 ggtggttaaa tggggttcca tgaccgcggc aaccggatcg tcgttaaaac cgatcaccgc
     1021 gattgaatcc gggatgacaa accgggcttc cttgatggcc tgataggcgc cgaccgccaa
     1081 aagatcgtcg atgcaaaaga cggccgaggg aagcggctga acctttaata gctctttggt
     1141 taggcggtag ccttcttcct gggtgagtgc accgcttcga atcaaggctg gatcgtattc
     1201 cacgccaaat tcccgcagcg ccaggcgata accatcaaac cggtcctggc agaaggtaaa
     1261 ctcctcgggg ccgttaatca ttgcgatccg ttgatggccg tgattcaaca gatgacgcac
     1321 cgcggtatag gaggcttgaa tattgtcgtt gttcaccgag tggcatttga tggactcagg
     1381 gatccggcct accactacaa aagggaagtc gcttttggtc aattccctga tgagttcgtc
     1441 atttacccgc gtggcgagga tgattattcc atccacccgt ttttcggcga tcatccgcat
     1501 ggcttgtttg gcttcttccg aataatcctc ggccgtggcc aacatcaggt tgtagtggcg
     1561 cttttgggta atggtgctaa tgccgcggat aatctcgggg aaaaaatgat ttaaaaaagc
     1621 gctggaagcg gagcgggata aaatcaggcc caatgtctga ctggagcggg tgatcaaact
     1681 ccgggcaatc atattgggat gataattcag ttctttcata acctttctta cccgggcttt
     1741 ggtctctgga ctgatccgtt ggctgtcggc gatcactcgc gatacggtgg aaggtgaaac
     1801 tgcggcgagc ttggcgacgt cttttatggt ggacataagg cttcctttcg ttacataaac
     1861 ggttgcattg aacctggctt tggcaagtac attacaactt ttttgaaaga gcacagaaag
     1921 gttggtcgaa tttgattcgg gttgaatata gtatttcccg atcgtattct tgatcggttg
     1981 acaaaatcct tttactggag agcattgatc tgattgtttc acggctttat tgcgtatgat
     2041 aaccgatctg actggtttgt gcaaacgttt gcttgacata agatatctct cccgaatata
     2101 atattaatgc actgaaagaa atatgaaagt caacgtacct ctctgaagcg gtttcttggg
     2161 ctcgtttgat tttcattgaa agcccttgtc gacttcaagg ggctaatatg cttgccggat
     2221 caaacatcaa ttgatgcatc atatcatatc gatactaaga ttaaggagtc attattatga
     2281 atcaaaaatt tatcagtgat gaatggaaaa ttatcgagcc tggattcgat ccggatacca
     2341 atcgtttttc ggagagtatc tttagcctgg gaaacgaaca catgggactg cggggctttt
     2401 ttgaggaggg ttattccggt gacagcctgc aaggaaccta tatcgccgga atattttacc
     2461 cggacaaaac cagggttggc tggtggaaga atggatatcc cgaattcttt gctaaggtag
     2521 tcaattcgac caattggatc gcaattgatc tggaactaga tgggacaaag cttgatctgg
     2581 cacgctgcag ggtatcggat ttccgccgcg aactggatat gaaggaaggg cgcctgaccc
     2641 gctcttttat ctgtcacagt gaggacggga aggatacggc tatagtcttt acccgtttcc
     2701 tgagtatggc cgataataac ctggcctgta tctccgcaac aatcaaaccc ttgaattatt
     2761 ccgggaagat catgttagca cccgggctgg acggaaatgt gaccaacgag gattcaaact
     2821 acggcgaaaa attctgggaa gaggtcgaca aggcagttgc gcaagattcg gcaatacttc
     2881 tcatgaagac caaaaagagc ggttatatcc aggcaactgc cttcggggtc cggacttttt
     2941 tggatggccg ggagattttc cccgagaagg cgctccaaca aaacgaggag aaagttggtt
     3001 atcggatggc gatcacggtc gaggccggtc aaaaactgga actccaaaaa ttcgtggccg
     3061 tctgtacctc acgggattat gatgttcatg aattacggaa tgtcacttta gacaagttac
     3121 ggcttgcctg tgagaagggt tgggcttcgc tctaccggga acacacggaa gaaatggccc
     3181 ggaaatggga agaaagtgat gtggtgattg agggggatcc cttggcacaa caaggaatcc
     3241 gctataatat ctttcaactc aaccaaacct atgccgggaa ggatccgcgg ctcaatatcg
     3301 ggcccaaggg cttcaccggg gagaaatacg gcggcagcac ttattgggat acggaggcgt
     3361 tttgtttccc cttttatctt tataccaatc aaaccgtggc ccggaacttg ttatactacc
     3421 gttatctgca tttggaacgg gccaaggaga acgcaaggaa gcttgggcta aggggcgcgc
     3481 tctatccgat ggtcaccatg gacggccagg agtgtcataa cgaatgggag attacttttg
     3541 aagagattca ccgaaacggg gccatcgcat acgcggttta caattatacc aattacaccg
     3601 gggataactc ttacctgttc caatacggaa tcgaagtgct cgtcgagatc agccggttct
     3661 ggacggaccg ggcgacttac aatccccaaa aagatcggta tatgatttta ggcgtgaccg
     3721 ggcccaacga atacgaaaac aatgtcaata acaactggta taccaatacc atggccgcct
     3781 ggaccttgga atataccttg caggcatgcc gggagattga agccgccgac cccgggcgtt
     3841 accgggaagt cgtttcaaaa tcggggatcg cggttgatga attctccaag tggcgcgata
     3901 tcgtaaccaa gatgtactac cctttcctcg aagagtcggg ggtttttgag cagaatgatt
     3961 tataccggga caaagaacag gtgctggtga aggatttgga tcccgcagag cttcccatca
     4021 acaaacactg gtcttgggac cggattttac gatcctgttt cattaaacaa gcggatgtga
     4081 ttcaagggat ctattttctg ccggaacggt tcgatcagga ttgcatgaga cgaaattttg
     4141 attattacga gccgcgaacc gttcatgagt catctctttc cgccagtatt cactcggtcg
     4201 ttgccagctg gatcgggtat aaaactaagg cttacgagct atatctgcgg accgcccggt
     4261 tggatctgga caattataac gatgataccg atgacggggt gcacatcacc agtatggccg
     4321 ggagttgggc cgcgatcgtc cagggatttg cgggagctct ggttcgtgat gatacgcttt
     4381 gtttgaaccc ctatgtcccc gatcagtggc agggatactc attcatgatc agtttccgcg
     4441 ggcgtaagct taaaatccgt gtcacccaag aagaactgca tatcgagcaa cttggtggag
     4501 aaccattgct cttgaacgta tccggcaggg agagcctatc tgaaaccggt aaagaccagg
     4561 tgattccgat tcaaccgcgg gtagctgtaa ccggctaagg gtaggggcag ttgaaaaaac
     4621 tttgtatacg cacagtgaaa aggagcttgg ccaaaagccg ggctcttttt tatttatctc
     4681 aaattaattc aagacttcgg ggtgaagtac cgaatgtcat tttatggtat aatgagaaaa
     4741 atatttgcga ctttttc
//
LOCUS       DASVMB010000242         9211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_397090,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000242 DASVMB010000000
VERSION     DASVMB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397090"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..349)
                     /locus_tag="VF531_07060"
     CDS             complement(38..349)
                     /locus_tag="VF531_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7713766.1"
                     /translation="MGKKYDVRYLPASQKDVTDIVDYIKQDNPSAAIKLIDEITEVIS
                     HLEDFPSIGSMPKDLRLQSLNYKILVIDNYLVFYTIKEEDNIVEIRRILHGKRRYSFL
                     F"
     gene            complement(333..605)
                     /locus_tag="VF531_07065"
     CDS             complement(333..605)
                     /locus_tag="VF531_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX7713767.1"
                     /translation="MPIIKPVSDLRNKFNQISEICHKEREPIFITKNGEGDMVVMSMA
                     LYEQQQALLELYQKLGEAEAQSLAGAPTISHKDFMKKLRDEYGQKV"
     gene            complement(713..934)
                     /locus_tag="VF531_07070"
     CDS             complement(713..934)
                     /locus_tag="VF531_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713768.1"
                     /translation="MPLPGHIQTTLPSAAIMAAAPALGLVSLLTDISTEMVYPLIPIY
                     LTSVLGATPAIVKYYNTSQYSHQRIDPNS"
     gene            complement(1004..2425)
                     /locus_tag="VF531_07075"
     CDS             complement(1004..2425)
                     /locus_tag="VF531_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX7713769.1"
                     /translation="MEGNWLSIIPPLTTIVNAIFTKRIISSLLFGLIVGSILKAGSLF
                     TGIFKAVEYLMGVLNSADNILVVLFLFSFGSLTEIFKMSGGIKGFARLAEKYVKTEKG
                     ALLSVWAVTPVTYLDCCFHTISSGTIAKPLIEKVNGSPERLALVVNVTSSQLIVLIPL
                     ATTYVGYIIGITGSAMREAGIPGSPYALFLESIPYNFYSWGMVILSLLVTLFGLGFGK
                     IRVKWGKPDQEGVHGMDEAHEQCKFEEKAPPKVWNLLLPLLVLVILVILLFWYTGYTP
                     GRSFVQALMHAQFEKSLFIATFGTLLLTAVFYLLQRIPLSEIDSHFLSGGTEMLPPIV
                     VLVISWALAAVTRDLGFQKFIMGAFGTGFPKLFIPVMVFIIGALTSYFIGSSWATWAL
                     IMPLGLPLAVNSGASIPLVMGAILAGGSVGDSVSFLGETPILTAAITELPLLDHIQTT
                     LPYAGIIAGLAAILFIVSQLMIG"
     gene            complement(2608..3717)
                     /locus_tag="VF531_07080"
     CDS             complement(2608..3717)
                     /locus_tag="VF531_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7713770.1"
                     /translation="MRFRWITAILLALLPVSGCWDAVDIDRRAIILALGLEMAPNNKI
                     LVSAQIPQSSFFPFSPGQSSEMPYQTLTGIAKTGYGATLGLESKTWKRLFFMQLKTVL
                     VNTALAEQGIKPYFEYLERNPQINPQSMVVLTETNCRDTLSVPLSSKDIPGMAIYNFL
                     KSEAKANQVFPMQTWEFLKRIEIRPIDAYLPIISYNSDEQVYKFDGIGVFHRDRLTGK
                     LSETESRMAGIVMKEAHNATFTVPVGKMGDISYQRLHWKREIAIIKTEPDFEFLINVS
                     AKGYGSEITRDPTELSPRQIKEIERVTENYLKRNMAATVHRLQAMNSDIIGFGELIRA
                     NKPELWKRLNWDQEYPRADFRVKVKFTLERVGKYR"
     gene            4494..4736
                     /locus_tag="VF531_07085"
     CDS             4494..4736
                     /locus_tag="VF531_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713771.1"
                     /translation="MAAGIRVFLHYLPTQKEWYYGATYIIGFAIVSAFLDKIFNQIGM
                     LHYNHWNPWYRFFVALAWFYGAAYHCNYLKARGKLG"
     gene            5066..6019
                     /locus_tag="VF531_07090"
     CDS             5066..6019
                     /locus_tag="VF531_07090"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX7713772.1"
                     /translation="MEKQRGSKVVIIGAGFVGSTFAYSLMINGVVAEIVMIDLNRERL
                     EGEIMDLNHGISFVRPVSVRAGAYEDCADADIIVITAGANQKPGESRIDLLSRNTEIF
                     RGIVDNIKAVHSQAILLIATNPVDIMTYITYKLSGFPAQRVIGSGTVLDTARFRYLLS
                     DHCHIDPTNVHAYIIGEHGDTEVPVWSLANIAGIRFADYCPVCGRNCETPLPKEDLFN
                     EVKNSAYRIIKAKGATYYAIGLALVEIVESILRDEYSVMTVSTLLDGEYGLHDICLSL
                     PCIINRKGLEKKIRLSLAPEEEAGLRRSGETLGEIQRQLKL"
     gene            6132..6398
                     /locus_tag="VF531_07095"
     CDS             6132..6398
                     /locus_tag="VF531_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713773.1"
                     /translation="MKPESDIQWELLTEVYQPDEAQLIGGLLNMAGIPVKIDREAIGE
                     IYGLTVGPLAKIVIRVPGERLTEAEQILNQSATGDGEDLPEAGK"
     gene            6456..7085
                     /locus_tag="VF531_07100"
     CDS             6456..7085
                     /locus_tag="VF531_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7713774.1"
                     /translation="MEYQRFIVGNLFTNCYLVWSDRTAGVIDPGGPVTELIRLIRERG
                     LSLQWIVNTHGHADHIMGNAELRREFGAPLLIHAADRPMLSSPDANLSSMLGDGFVSP
                     DADRTLKHGDRLALGGEQLTVLETPGHTPGGISLVAPGLVFSGDALFLESIGRTDFPG
                     GDRRQLVNSIRTHLLTLLPETVVLPGHDAPTTIGYEARFNPYLAATDEV"
     gene            7075..8556
                     /gene="hemZ"
                     /locus_tag="VF531_07105"
     CDS             7075..8556
                     /gene="hemZ"
                     /locus_tag="VF531_07105"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HEX7713775.1"
                     /translation="MRYSIQGAAPRQLPNIEAALKIVDHHVILVPETDRPDLRLMMEY
                     HDSPALKVRVVAPDLPEPGGVWEYQDREIEDPRYQQDDRSQRLKELIRLGIIRVVGGF
                     LGTRPPWGILSGVRPTKIFHYLRDKGFSAAEIRDRLVSIYGLIPEKADLLVVIGVRQE
                     AHLHPPRDLVGIYLGIPFCPTRCQYCSFAAVSLETHSHQVQPFLNALDREVAATADLC
                     HQLGLAIDTLYIGGGTPTSLDEAAFRHMISHFARAFAATPLREFTVEAGRPETITPEK
                     LALMKEAGVTRISVNPQSMNQATLDRVGRKHTVAQIREAVRMVREQTDFLLNMDLILG
                     LPGETGSGFLESLEQVLELKPENITIHTLAQKRAAAWNSRFDSMDLAAPPDLTSAAAK
                     GFNLTNACGFHPYYLYRQRHILADLENIGFAKNGSECLYNIRMMEERQTILGLGGGAI
                     TKWVFPPEYRVARLQNPKCPATYVQRLEELIVKKAQQSRILLG"
     gene            8753..>9211
                     /locus_tag="VF531_07110"
     CDS             8753..>9211
                     /locus_tag="VF531_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7713776.1"
                     /translation="MSIRKVTQKLLAPVIIVLVVAMTVGVFYISPMMTKDNGGYKGPA
                     ARLNGEKIKDADFNSVMFSVSQQAMQMAQYGIAYTDAQIRDQALDSAIGDLAFKQEMK
                     KEGSKINVSGAEVDKFIKDRWPTEEELQSAMQQYGQADKGQFKKWIADQLK"
BASE COUNT         2455 a         2096 c         2194 g         2466 t
ORIGIN      
        1 ccgtggcctt ccgggccgct gggtagagta agaaagttca aaataaaaac gaatatctcc
       61 ttttaccatg tagaattcgc cttatttcaa caatattatc ttcttcttta atagtgtaga
      121 agaccaaata attatcaatc accagtattt tgtagttgag gctctgtaag cgtaaatcct
      181 ttggcataga tcctattgac ggaaaatcct cgagatgaga aattacttcc gtaatttcat
      241 caattaattt tattgctgca ctggggttgt cttgttttat atagtctaca atatcggtta
      301 catctttttg agaagctgga agatagcgga cgtcatactt tttgcccata ctcatccctc
      361 agctttttca taaagtcttt gtgagaaatg gttggggcac cggcaagact ttgcgcttcc
      421 gcttcaccca atttttggta taattcaagt aaagcctgtt gttgttcata caatgccata
      481 ctcattacca ccatgtcgcc ttcgccgttt ttggtgataa aaatcggttc tctttccttg
      541 tggcagattt ctgagatttg attgaatttg tttcgtagat ctgagaccgg tttaataatg
      601 ggcattcaat ccaccgccta tcataaaaga tatatatatt atatcacgat aatgatatta
      661 aatgccatga ttaaaggtta tttttgaaag tggccgtcat ccgtgacgtc cttcaactgt
      721 ttggatcgat tcgttgatgg gagtattggg aagtattgta atacttgaca atggccggtg
      781 ttgcccccag gacggatgtt aagtagatgg gaatcaacgg gtagaccatt tccgtgctta
      841 tatccgtcag taagctgaca agtccgaggg cgggagccgc agccataatt gcggcggaag
      901 gtaaagtggt ttgaatatga ccgggtaacg gcaattcagt gatggctgtt gttaaatcgg
      961 tgtctctccg agaaaagaaa cgttgtgata tttaatattt aatctaccca atcattagct
     1021 gagacactat aaaaagaata gccgccaaac ccgcaataat tccggcgtaa ggaagtgtgg
     1081 tctgtatatg gtccaaaaga ggcaattcgg taatggctgc agttaaaatg ggggtttccc
     1141 caagaaagga aacactatct ccaaccgagc cacccgctaa aatagcgccc ataaccaaag
     1201 gaatcgacgc tccggagtta acggccagcg gtaatcctaa tggcatgatt aatgcccagg
     1261 ttgcccagga ggaaccgata aaataggatg tcaaagcgcc gataataaat accatcaccg
     1321 ggataaacaa tttaggaaat ccggtgccaa aagcccccat gataaatttt tgaaaaccga
     1381 ggtcccgggt cacggcggcc aaagcccaac ttataactaa aaccacaatt ggaggcagca
     1441 tttctgtccc tccgctcaaa aaatggctat cgatttcaga taaaggaatt ctttgcaata
     1501 aataaaaaac agccgtcagc aacagtgttc caaaagtggc gatgaacaag gatttttcaa
     1561 actgggcatg cattaacgcc tggacaaaac ttcggcccgg tgtatatccg gtataccaaa
     1621 acagcaagat gaccaggatg actaaaacta ataaaggcaa cagcagattc caaaccttgg
     1681 gaggcgcttt ttcctcgaat ttacactgtt catgggcttc atccatccca tgaacgcctt
     1741 cttgatcggg tttaccccat ttcactctga tttttccgaa acccaatcca aaaagggtca
     1801 ccaataaact taaaataacc ataccccagg aatagaagtt ataagggata ctttccaaaa
     1861 acaatgcata gggtgagccg ggaattcccg cttcccgcat cgccgaaccg gtaatgccga
     1921 taatatatcc cacataagta gttgccaatg gaattaacac aataagctgc gaggaagtga
     1981 cattgacgac caacgctaaa cgctccggag aaccattcac cttctcaatg agaggtttgg
     2041 cgattgtacc gctggaaatg gtatgaaaac aacaatctaa ataggtgacg ggtgttaccg
     2101 cccaaacgct aaggagcgct cctttttcag ttttcacata tttttcagcc aaccgggcaa
     2161 aacccttgat tccaccggac atcttgaaaa tctcggttaa agatccgaag gaaaataaaa
     2221 agaggacaac taaaatatta tccgctgaat taaggacccc cataagatat tcgaccgctt
     2281 taaagatccc ggtaaataaa cttccggctt ttaaaatact tcccactatc aaaccaaata
     2341 ataatgagga aataattctt ttcgtaaaaa tggcattgac aattgtcgtt agtggaggaa
     2401 taatcgatag ccaatttcct tccaatacac agcccccttt gctgattatt atttgcccga
     2461 aaaaatgaat ccaaactttt tcatttattt tttacagata gttatttcgc cagctatgga
     2521 cttccgaaag agatctgtct gttattcgtc tgcaatgctt aacgatatag cgcgcctttt
     2581 tccctaacca ggcctattgt gagtcaatta tctatatttc ccaacccgct ccaaagtaaa
     2641 tttgaccttt acccggaaat cagcccgggg atattcctga tcccaattga ggcgtttcca
     2701 cagttcgggc ttgtttgcgc gaataagttc tccaaaaccg ataatatccg agttcatggc
     2761 ttgaaggcgg tgaactgttg ccgccatatt tcttttcaaa taattctccg taacccgttc
     2821 aatttctttg atctgtcttg ggctcagttc cgttggatcc ctggttattt ccgaaccata
     2881 acccttggcg ctaacgttga ttaaaaattc aaaatcgggt tctgttttaa taatcgcgat
     2941 ttcacgtttc caatgtaatc tttgatagct gatatctccc attttcccga ccggaactgt
     3001 aaaagtggcg ttatgggctt ctttcataac tataccagcc attcggcttt cagtttctga
     3061 aagtttacct gtgagacgat cccggtggaa aaccccaatc ccgtcgaatt tatagacttg
     3121 ttcgtccgag ttgtaggaaa taatcggtaa atatgcatca atgggcctta tttcgatcct
     3181 cttaagaaac tcccacgttt gcattggaaa tacttgattt gctttggcct ctgactttag
     3241 aaagttataa atagccatac cgggtatatc ttttgaactt aaaggaacgg ataaagtatc
     3301 ccggcagttt gtttcagtca agactaccat actttgggga ttgatttgcg ggttcctttc
     3361 caaatattca aaatatggct taattccttg ttccgctaaa gcggtattta ccaaaaccgt
     3421 tttaagttgc atgaaaaaaa gccttttcca tgttttgctt tctaatccta aagtagctcc
     3481 ataaccggtt ttggcaatac ccgtgagtgt ttgataaggc atttcggaag attggcccgg
     3541 ggaaaaggga aaaaaactac tttggggaat ctgagcggag acgagtattt tattattcgg
     3601 ggccatctcc agtccaagag cgagtataat tgcacggcgg tcgatatcta ccgcatccca
     3661 gcatcctgag acgggcaata gggccaataa tattgccgta atccatctaa accgcattta
     3721 cgttcgtacc ccttttgtta aaaattaatg caaaaatcca aagcaaaaat ggatatccga
     3781 agaaagatat ccagccgttt tgagcaacaa aatctacgaa catttttgtt tgaatcacac
     3841 cggcaggcaa cataatgata ataagtttta ctggttcggt ttataatgat cggttcacgc
     3901 gctctgtgga ttagctttat gtaatatatt ctttgatttg gccatgataa aatatggcgc
     3961 gactggataa tctaaaaaaa tccctctcat catttactct atggcggtgt catcctattt
     4021 gtttaaagac cgaattgtcc tcggttccct tgccggaatg atcactcata ttaaccccaa
     4081 cgatgatccc aatttccgat tcgccgcttt atttgtccat caattttttg gcttattgac
     4141 cacatatttg atttaccatt actcagataa agaacacaaa aaagcatgac gcaggtacac
     4201 aaaagtcaga gattaatccc ctgggaatgg agttgaattg agatgagtga agttcaggtt
     4261 ctgggggtac cggttgaata ctattaccct ggttttgcgg tattaatgtt tatactcctt
     4321 attgttttcg tacccaagga aaagttcaaa tcatatttct ggctcgcttt gatctggggt
     4381 tgtcttggga gtatctttgt tggaacaatt tttgggggta tcttaaaatt gtttcggtgg
     4441 gaatacgcca tgccgttttt attttacggc catccgattt ggattaatct ggcatggctg
     4501 ctggaattcg tgttttttta cattatttgc cgactcaaaa agaatggtat tatggtgcaa
     4561 cctatataat tggctttgca attgtcagtg cgttcctgga taaaatattc aaccagatcg
     4621 gaatgcttca ttataaccac tggaatcctt ggtatcgttt ttttgtcgct ctggcctggt
     4681 tttacggagc agcctatcac tgcaattatt tgaaggcgcg agggaaactt gggtaacaag
     4741 aaagttacct gcgatggatg aatgtaactt tagtcacaaa agggaagaat tatgtcaaat
     4801 attaatcccg gaattttttg tcctggggcg tcaagtaact tgtgagtttt gttatatcaa
     4861 aatatctgat gctcaaaata cgttttccta ttccataagc gcttcgttat cattcatgtg
     4921 atataaatgg gatgaaataa tctggagcag ttttctttga taaaatcaaa taagtatttt
     4981 ataatattac ccagaactcg atcgaaaact ccaatcggtt ttttggatga tttggataga
     5041 ataaatgaaa ggtggagcga tgatcatgga aaaacaacgc ggcagcaagg tggttattat
     5101 cggagccggt tttgtcggtt ctacatttgc gtacagtctg atgattaacg gagtggtcgc
     5161 ggagatcgtg atgatcgatc tgaaccggga acgtctcgaa ggcgagatta tggacctcaa
     5221 tcatgggata tcctttgtcc gtccggtttc ggtaagggca ggagcttatg aggattgcgc
     5281 cgacgccgat atcatcgtca tcaccgcagg ggccaatcaa aaacccggtg agtcccggat
     5341 tgacctgtta tccaggaata ccgagatttt cagggggatc gtcgataaca taaaagccgt
     5401 tcattcccag gcgattctct tgatcgccac caatccggtg gatatcatga cctacatcac
     5461 ctataaattg agcggttttc cggcacaacg ggtcatcggt tccggaacgg tgttggacac
     5521 tgccaggttc cgctatctct tgagcgatca ctgccatatc gaccccacca acgtacatgc
     5581 ctacatcatc ggagagcacg gcgacaccga ggtacccgtt tggagcctgg cgaatatcgc
     5641 cggaatccgg tttgccgatt attgcccggt ttgcggcaga aactgtgaaa cccccctccc
     5701 caaagaggat ctgtttaatg aggtcaaaaa ctccgcttac cggatcatta aagccaaagg
     5761 tgcaacctat tatgccatcg gtctagcctt ggtcgagatc gttgagagca tactgcggga
     5821 tgagtattcc gtaatgacgg tctctacctt actcgacggg gaatatggat tgcacgacat
     5881 ctgcctcagc ttaccctgca tcatcaatcg caagggtttg gaaaagaaga tccggctttc
     5941 attggcgccg gaggaagagg ccggattgcg ccgctccggt gagaccctgg gggagattca
     6001 acgccagctc aagctataaa ccggcattca ttcataacca tttgacttta taagataaaa
     6061 ttccggatca tatcagggtt gcgggacgga gacagaaact ccggcagcat aggatcaagg
     6121 agtgtggcga aatgaaaccc gaatccgata tccaatggga actgcttacc gaggtttatc
     6181 aaccggatga ggcgcagctc atcggggggc tgctcaatat ggcggggatc cctgtcaaaa
     6241 tcgatcggga ggcgatcggt gaaatatacg gcctgaccgt gggaccgctt gccaagatcg
     6301 tgattcgggt gcccggcgag cgactgacgg aagcggagca gatcctgaac cagagtgcga
     6361 ccggtgatgg cgaggactta cccgaagccg gcaaataact tgcccgcgga cggctcgaac
     6421 gggtgaatcc ttacccggta ctgcaggagg ataccatgga atatcaacgg tttatcgtgg
     6481 gcaatttatt caccaactgt tacctggttt ggtcggacag gaccgccggg gtaatcgacc
     6541 ccggcggtcc tgtcactgag ttgatccgct tgattcggga gcggggtttg tcattgcaat
     6601 ggattgtcaa cactcacggc cacgccgatc atatcatggg aaacgccgaa ttacggaggg
     6661 aatttggagc gcccctcctg attcatgcgg ccgatcggcc gatgttgagc tccccggatg
     6721 ccaatctatc ttcaatgctc ggtgacggct ttgtcagtcc cgacgccgat cgtaccctca
     6781 agcacggcga ccggttggct ttgggaggcg aacaacttac agtgcttgaa actccgggac
     6841 acaccccggg tgggatctca ctggttgcac ccggccttgt cttttccgga gacgctctgt
     6901 ttttggagag tatcggcagg accgactttc ccggaggcga ccggcggcaa ctggtgaatt
     6961 cgatccgcac ccatcttttg acgttactgc cggagacagt agtcttgccg gggcacgatg
     7021 cgccgaccac catcggctat gaggcgcgtt tcaatcctta tctggcggcc accgatgagg
     7081 tatagcatcc aaggggccgc tccccgtcag ctccccaata tcgaagccgc gctgaaaatt
     7141 gtggatcatc atgtaatcct cgttcccgaa accgaccgcc cggatttacg tttgatgatg
     7201 gagtatcacg acagccccgc tcttaaggtt agggtagtag ccccggacct cccggagccc
     7261 ggtggagttt gggaatacca ggatcgggaa atcgaggatc cccggtatca gcaggatgac
     7321 cggtcccaac ggctaaagga actgatccgc ctgggaatca tcagggtggt cggcgggttt
     7381 ctcggaacgc gcccgccctg ggggatccta agcggtgtcc ggcccaccaa gatctttcat
     7441 tatctgcggg ataagggttt tagcgccgcg gagatccggg ataggctcgt ctccatttac
     7501 ggtctgattc cggaaaaagc cgatctgctg gtcgtgatcg gagtgcgcca ggaagctcat
     7561 ttgcatccgc cgcgggatct ggtcggcatc tatctgggaa ttcccttttg ccccacgcgg
     7621 tgtcaatatt gttcatttgc cgcagtttcc ctggaaaccc acagccacca ggttcaaccc
     7681 tttttgaatg cactcgatcg ggaggtggct gcgacggccg atctctgtca ccagttgggt
     7741 ctggccatcg acacccttta tatcggaggg ggcaccccca ccagtttgga tgaggcggcc
     7801 ttccgccata tgatctccca ttttgcccgg gctttcgccg caacgccgct ccgggagttt
     7861 acggtggaag cgggaaggcc ggagacgatt accccggaga aactggcgct gatgaaggag
     7921 gccggtgtca cccggattag cgtcaatcct caatcgatga atcaggccac cctggaccgg
     7981 gtcggccgca aacacacggt ggcccagatt cgcgaggctg tgcggatggt acgggaacaa
     8041 actgattttc tgttgaatat ggatttgatt ttgggattac ccggagaaac gggctccggc
     8101 ttcctcgaat ctttagagca agtgttggaa ttgaaaccgg agaatatcac cattcatacc
     8161 ttggcgcaaa aacgtgcggc cgcatggaac tcccggtttg actccatgga cctggcggcg
     8221 cctcccgatc taacgtctgc agcggccaaa gggttcaacc tgacgaatgc ctgcggattt
     8281 catccgtatt acctttacag gcaacgtcac atcctggcgg atctagagaa tataggtttc
     8341 gccaaaaacg ggagcgaatg cctttataat atccggatga tggaagaacg tcagacgatc
     8401 ttagggcttg gtggcggagc gattaccaaa tgggtctttc cgcccgagta ccgggtagcc
     8461 cggctgcaaa atcccaagtg cccggcaaca tatgttcagc gtctggagga attgatagtc
     8521 aaaaaagccc aacagagccg gatacttctt ggttgacatt taggttgaca ttaggtaaaa
     8581 tattaaaagt gatctgctct atccttgaaa tatcaagtga atatcggttt tcaaattcac
     8641 tgttttcaaa gatggggaga gagtatgggt tagggctcat ttttttttta acactggtct
     8701 gacaccaagt ttggtttgat agatttgata agggggaatt tacgtgggga tcatgtcgat
     8761 ccgtaaggtg acccagaaat tattggctcc ggttatcatt gttttggttg tggccatgac
     8821 ggttggcgtg ttttacatca gcccgatgat gaccaaagat aacggcgggt ataaaggacc
     8881 ggctgctcgc ttaaatggcg agaagattaa ggatgcggat tttaactcgg tgatgtttag
     8941 cgtttcccaa caggccatgc agatggccca gtacggaatc gcttataccg acgctcaaat
     9001 ccgtgatcaa gcattggact ccgccattgg cgacctggct ttcaagcagg aaatgaagaa
     9061 agagggctcc aagatcaatg ttagcggcgc tgaagtggac aagttcatca aggatcgctg
     9121 gccgaccgag gaagagctcc aaagtgcgat gcaacaatat ggccaggctg ataagggtca
     9181 gttcaaaaaa tggattgccg atcagttgaa g
//
LOCUS       DASVMB010000243        13870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_397170,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000243 DASVMB010000000
VERSION     DASVMB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397170"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VF531_07115"
     CDS             <1..374
                     /locus_tag="VF531_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012862177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7713777.1"
                     /translation="VLTLPGVAGFVLSLGMAVDANVIIFERIKDELRNGKRVRAAVEA
                     GFDRAYVAIIDSNVTTVIAAGVLYFFGTTATQGFAVTLTLGVLVSMFTAIFVTKALIE
                     WWIDRDPDRHAKQFGFKEVAE"
     gene            374..1342
                     /gene="secF"
                     /locus_tag="VF531_07120"
     CDS             374..1342
                     /gene="secF"
                     /locus_tag="VF531_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7713778.1"
                     /translation="MNLMKYRWVFIGVFLLTMALSVIFLFFNTFNGFGTKPGSSLNLG
                     IDFTGGTKIYFPVPHAVTSSQVAAVLDDLQATQLPKLKYNAPQPGQNPDSTGVVRYKV
                     MVYTNFLNDSEQGIVIKALEAKFGKAEKNQGLEVTRIDPLIGKELVNKSLLAVLIASG
                     LMLVYIWFRFELVSGIGAVIALLHDCLLVMGLMALLGMEMNAAIIAALLTILGYSIND
                     TIVVFDRIRENLKYRVKGTSFVELVNDSLLQTFRRSLNTGMSAIMAVLVLFVLVPSLK
                     ELCFALLVGMTSGTYSSFFIASPVWAIYKDWQERKKLAAKPAVATR"
     gene            1829..2539
                     /locus_tag="VF531_07125"
     CDS             1829..2539
                     /locus_tag="VF531_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7713779.1"
                     /translation="MIAIIMSILSITLIDLVLSGDNAAVIGLAIKDLPHEERKSAALW
                     GAGGAIVLRVFFTVLATLLLKIPYLNAIGGVILLWITWNLLNSHDDENQDDHGKTVTK
                     FWKAVGTIILADLSMAFDNIMGVAGAAHGEIYLVIFGLALSIPILVLGASWLATLMEK
                     FPIIIYLGGAVLVHTSLAMIFHDKALYLFHYLNEFTIQIISWLAALSVLAIGVFKIGL
                     FTKSNQQLASTKDNTNEN"
     gene            2737..3030
                     /locus_tag="VF531_07130"
     CDS             2737..3030
                     /locus_tag="VF531_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713780.1"
                     /translation="MGLSMSNEMMQWLDISLLGIIALFLLILIGLAGRVIALLNELKD
                     IKSATDYQSAAPACRDEEASVFEEDLAVIMAIMAQKYPALKDAAIQVRPVKMD"
     gene            3069..3470
                     /locus_tag="VF531_07135"
     CDS             3069..3470
                     /locus_tag="VF531_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7713781.1"
                     /translation="MQKFRVSVNGRIYEVGVEELAPGTNQAPLAASAEAPSVTNTSGS
                     APVTPAPVASSDASLDPGDKPVNAPLSGAIIHVNVKPGEKVKYGQVLLTLEALKMENE
                     IVAPENGTIKAIYVQAGSSVNVGDVLLSLVP"
     gene            3501..4838
                     /locus_tag="VF531_07140"
     CDS             3501..4838
                     /locus_tag="VF531_07140"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEX7713782.1"
                     /translation="MAQPIKITETVFRDAHQSLWATRMKTEDMMAVAEAIDEIGFYSL
                     EAWGGATFDSCLRFLKEDPWVRLRRLKAVIRKTPLQMLLRGQNILGYRHYPDDVVREF
                     CHRAVDNGIGIIRIFDALNDIRNMEVAIKASKEAGAHVQGTVVYTISPVHNIESYIKL
                     AKELTQLGVDSICIKDMAGLLKPYVAEELIKGLKKAVKVPIQLHGHYTSGLVSMTYLK
                     AVEAGVDVVDTALSALAMGTSQPATEAIVAALQGTEFDTGLDLAKIAPINQHFKEIKK
                     NYQNVSAPVEVDTAVLNYQIPGGMISNLRNQLAGQNAADRYGDVLKELPQVRKELGYP
                     PLVTPTSQMLGTQAVLNVITGKRYSVVPKEIRDYVKGLYGRPPAPIDPEIKKIVIGDE
                     EPITHRPADDLEPLLDKARKEIMPYYQQEEDVLSYVLFPEVAMEFFKFRVGNK"
     gene            4897..6021
                     /locus_tag="VF531_07145"
     CDS             4897..6021
                     /locus_tag="VF531_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX7713783.1"
                     /translation="MEQLINELFKGALYLNWQQLIMMAIGGILIYLAIAKDYEPMLLL
                     PIGFGAILANIPFSSAIGHDGFLSILYNAGIYTELFPVLIFIAVGAMIDFGPLLQNPF
                     MLFFGAAAQFGIFATMMVALLIGFDLKQAASIGIIGAADGPTSIYVATKFAKDLLAPI
                     SVAAYSYMSLVPLIQPPVIKLLTTKKERLIRMEARQVNIPKIVRILFPILVTVVAGII
                     APISVALVGALMFGNLIRECGVVERLSKSAQNELSNLVTLLLGITIGSTMTARQFLNP
                     ATIMIMVMGIVAFIFDTAGGVIFAKFLNIFLRKKVNPMVGAAGISAFPMSARVIHKMA
                     QKEDPTNFILMQSISANVAGQLGSIVAGGLLLAIVPVLLK"
     gene            complement(6441..7295)
                     /gene="dapB"
                     /locus_tag="VF531_07150"
     CDS             complement(6441..7295)
                     /gene="dapB"
                     /locus_tag="VF531_07150"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7713784.1"
                     /translation="MKICLLGLGRTGSVVAEQLLKTDDLELAAVITSPGSSKANRSLR
                     EFINCDTDLVIRDASSLAEECRKTPFTVAIDFTKPEASLHNARILAEHDVHLVIGTTG
                     FNKLQLHELQGLVRRHHIGLVYAPNISVGINLLLILVQTLAALVPQYDIEITELHHRY
                     KKDAPSSTALKIASTVNAARGIKGREKLCFGRKGATPREAGEIGIHAIRAGGIAGVHR
                     ILFAGDNDELEITHRSYSRAVFAEGALEAARFVAPRKGFFNMEGVLAFTDTPDTEAGP
                     DNLQFNYY"
     regulatory      complement(7419..7594)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(7833..9194)
                     /locus_tag="VF531_07155"
     CDS             complement(7833..9194)
                     /locus_tag="VF531_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713785.1"
                     /translation="MSRQALSTSQALRRSLTLVITGVSFGIVFFTMINGAPLTGYLRA
                     LGMNDLLYGIIMALPLLGNIAQIPTAYFLETRGHLKSVFLISGFFRLLWIPITLVPFF
                     IPGLHRPERLAIVTALIAVWAGTNAMVGVTFNSWMGGLVPDGIRGRFFARRYMIYTIV
                     GALAGLAAGKFLDRVPGFTGFAILFGIGSLFGAGDITSFFGVESPPQNLSQQRSSFKQ
                     LFRLPFTDRRYMKFIRFVAVWNFGFNFAAPFFNVYMLESLKMNYFKIFLLSQIISNLA
                     TVLFIQVWGRIADRYGNKPVVAICCAINIMTPFIWCFVTPGNYGLLIPVIYTLIGLSQ
                     PGLDVTLNNLSIWLAPEQNRSVFIATYALITSLAGSVLAFICGGAFMQFTRAFFTSHS
                     LPFLMGESFSSFQGLFILSALVRLVALCCFLPGFSEDHSRRASGVWNTWRGRLINRFS
                     RFH"
     gene            9302..13768
                     /locus_tag="VF531_07160"
     CDS             9302..13768
                     /locus_tag="VF531_07160"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7713786.1"
                     /translation="MNHPVLEQFHPAVATWFQEQFPAPTPPQIQGWPLIAAGQNVLIL
                     APTGSGKTLAAFLKCLDWLYQEAAAGHRIDDGVKVLYISPLKALNHDIHRNLELPLSG
                     IRETAERMGMDLPVLQTAVRTGDTPSAERQRMLRRPPQILITTPESLFLLLSSQARRI
                     LGTVRYVIVDELHTVFANKRGAHLALSLERLEQLAGSSQPLVRIGLSATIRPLDEAAA
                     FLGGNSWDEAGEILKPRPVAVVDAGEKKTWDLQMILPLPDLRELPEKSIWPPIYQKLL
                     DMVRAHRTTLVFVNNRRQAERITANLNELAGKEIARTHHGSISREVRLEVERLLKSGE
                     LSCIVATASLELGIDVGSIDLVVQIESPKEVSRGLQRVGRAGHVVGLPSKGRIIPKTR
                     ADLLEAAAILREMRAGRVETSKAPRNCLDVLAQQLVAITATGESPVDGVYHLTRCAYN
                     YRDLSRSEFENVLAMLSGSFSTREYTELKPRLYWNKDAGVIGPDPYGKRLVFSSGGTI
                     PDRGYFGVYLLGSGVRLGELDEEFVFERRLNERFVLGTSLWKIEEIRQDRVIVSPARK
                     GEAIVPFWKADQGGRPFELGQRIGAFLESAEARLEAPDFIEWLEEEFGLTRDAALNLR
                     RYLTDQKRALGFLPTHKRLVVEEFPDEVGEWRVVIHSPFGLRLHSALGLLVREEWQQR
                     RGITVEEMASDDGIMFEFPGGDEPPRLPWEELPREGLEERLAGLVSSTPLFGAVFRQA
                     AQLSLVMPRGGYGRKRNPLWLTRLKAGNLLQVVSKYRDFPLVVETYRQILQDYFDIAA
                     LREVLAGLAGGSLEVHTCRRDRPSPFAQGYLLNFVNSFMYESDTPKGERRLQLFGLGR
                     DTLRALVGAEGFRELFTPEAVQAVARKAQGLEVLDREVDTERVRYWLERRGDVGTEEL
                     ETLFPENAVRIREELQRLAASGQAVWIRRDPGGPQLVMAGTLRDEYLAALDDLRLENP
                     VALRVNEEETNRSSRAPSALMSREEARRRIVERYLRTHGPFGVAEICRRYGFAATEVA
                     TELSILAAAGLAEPGEFVPGGRGEEWCSPELLREIHRRSLAQARKEIEPRKPKAYAVL
                     VAGWQGVGCKRVGLEGLAETLTQLAGVWLPAADWEGGVFPVRVRDYRPIILDQLIASG
                     QIGWEARGEGNNLKIRFYNTTSIVMAPDTIPVGAGLTEPGVSPRQSVGELSAGAVEIL
                     DLLRNRGALTLPQILQVKGGGTMTVWEVLEELVRAGRISNDSFGPVRYLLETAVKDRS
                     GARGVLQPAVLARMGRWSLLPELGEMEREVRVEGLLNRYGMVCRETVGPEELSWGELY
                     PGFELLENIGKARRGYFVEGLGGIQFAWTAAVERLRRDPEPGKCWALGWHDPANVVRI
                     FPEWPGAEELLRATPDWVVYEAGEPVLVASGKKLKLQAVTERTPQSLTGALGELVRLL
                     AGAYPDQKIAVVQWNGEQVLESPAEEVLVGVGFERGYRELTLWPSERNLQFK"
BASE COUNT         3410 a         3093 c         3871 g         3496 t
ORIGIN      
        1 gggtgcttac tctacccggt gtggccggtt ttgtgctctc tttaggtatg gcggtcgacg
       61 ccaatgtgat catcttcgag cggatcaagg acgagctgcg taacggcaaa cgggtgcgcg
      121 cggcagtaga ggccggtttt gaccgggcct atgtcgccat catcgattcc aacgtcacca
      181 cagtcatcgc ggcgggagta ctgtatttct tcggtaccac ggcaacccag ggttttgccg
      241 tgacgctcac acttggtgta ttggtcagta tgttcaccgc catcttcgtc actaaggcct
      301 tgattgaatg gtggattgat cgtgatccgg accgtcacgc caaacaattt gggttcaagg
      361 aggttgcaga ataatgaacc ttatgaaata tcgctgggta tttatcggag tctttttgct
      421 cacgatggcc ctgagtgtaa tcttcctgtt cttcaatacg ttcaatggat ttggcacgaa
      481 acccggctcc tccctgaacc tggggatcga cttcacaggc gggaccaaga tttacttccc
      541 ggtgccgcat gcggtcacct catcccaagt tgcggcggtg ctggatgatc ttcaagccac
      601 ccagttgccg aagttgaaat acaacgcccc gcaaccaggc cagaatccgg actctaccgg
      661 ggtggtgcgt tacaaggtga tggtttacac caatttcctg aacgactcgg aacaggggat
      721 cgtcatcaag gcgttggaag ccaagtttgg aaaggccgaa aagaaccagg gcctggaggt
      781 cacccggatc gatccgctca tcggaaagga attggttaat aaatccttat tagcggtgct
      841 tatcgcttcc ggtttgatgc tcgtctatat ctggttccgt tttgagctgg tttccggtat
      901 cggagcggtt attgccctgc tccatgattg tctgttggtt atgggcttga tggctttgtt
      961 gggcatggaa atgaatgccg ccatcatcgc ggcgctcctg accatcttgg gatactccat
     1021 taacgacacc atcgtggtct tcgaccggat ccgggaaaac ttaaaatacc gggttaaagg
     1081 gacatcattt gtggaactgg tgaatgacag tcttctccag actttccgcc gttctctgaa
     1141 taccggtatg tcggccatca tggcggtatt ggtactgttc gttttggttc ccagtcttaa
     1201 ggaactctgt ttcgctttac tggtaggcat gacttccgga acctattcct cattctttat
     1261 cgccagtccg gtctgggcaa tctacaagga ttggcaagag cggaagaagc ttgccgccaa
     1321 accggcggtc gcaactcgct aaaaacgccg cacgataaat caaaacttgt caaactaaaa
     1381 aagctgttcc aggagactgg gcagcttttt tgcgttcatt atggagctaa tccggcgggt
     1441 gaaagtcctg agtgacaggc aggttgatat cggagtttgg gcctcaaaaa tctaacacga
     1501 gagctgccta attaaatcgc tcaaattttt atgaaatatt taatatttaa ggctggttaa
     1561 gcgatgttaa tattagttta atgaaattat cattaccttg acatagctat ttaccgaaga
     1621 taaacttatt agggcaaaaa gtttcaaaat tgatttgtaa attttaacct ataatataaa
     1681 tattgaatta tatagctaaa aaatggtaat tttgtccatc taaaaggggt gaatcattca
     1741 aaatcatatt ttatcttaaa taagaacttt tgtttcaaca gttcctcaga ttatgcaagt
     1801 tattcttgga agtgaggtta taacaatcat gattgccata ataatgtcta tattatcgat
     1861 tacgttaatt gatctggttc tttcgggaga taacgcggct gttatcgggt tggctattaa
     1921 agatttgcct catgaagagc gaaaatcggc tgctttatgg ggggctggcg gggcaattgt
     1981 attaagggta ttttttacgg ttttggcaac tttattatta aaaatcccct atttaaatgc
     2041 tatcggtgga gttattctgc tgtggataac ttggaatttg ttaaattctc atgatgatga
     2101 aaaccaggat gatcatggaa aaaccgtaac taaattttgg aaagcggttg gaacgatcat
     2161 tttagccgat ctttcaatgg cttttgataa catcatgggt gtagctggag ccgctcacgg
     2221 agagatctat ttggtaattt tcggactggc tttaagcatc cccatcttag tgctgggagc
     2281 aagctggctt gcaacattaa tggagaagtt tccaattatt atttaccttg gcggcgcggt
     2341 gctggtacat acctcattag cgatgatttt tcatgataaa gccctttatc ttttccatta
     2401 tctgaatgaa tttaccattc aaatcatttc ttggcttgcc gcactctctg ttttggcaat
     2461 tggtgtattt aaaatcggtc tgtttacaaa atccaatcaa caacttgcat ccaccaagga
     2521 taatacgaat gaaaattgaa cgggtccaag agttgcctcc aattggaggc gtactctaca
     2581 gggaaaagtt cagttttcat taataaacag gattttccga gaaataatag aagaccaggt
     2641 ggtttcattt ttttgccctg aagtgggaat ttcgatttcc tattatccta acaaccatag
     2701 agcaatggaa aatgagcaaa ggaggtaatt tccgagatgg gattatcgat gagcaatgag
     2761 atgatgcaat ggctggatat ctctttattg gggatcattg cactttttct attgattctg
     2821 atcggtttgg ccggacgggt tattgcatta ttgaatgaac tcaaagacat caaatcagcg
     2881 accgattatc aatccgccgc tcctgcctgc cgagacgaag aggcaagtgt atttgaagaa
     2941 gatctggcag tcatcatggc gattatggcg caaaagtatc cggcattaaa ggatgccgct
     3001 attcaagtaa gaccggttaa aatggattga cagttttaaa atacttaaaa tctacaggag
     3061 gaatggaaat gcaaaaattc agagtttccg ttaacggaag aatatatgaa gtgggagttg
     3121 aggaattagc tcccggtacg aatcaggcac cgctggccgc ttctgcggag gcgccatccg
     3181 ttaccaatac atccggctcc gcaccggtta cacctgctcc ggttgcatcc tccgatgcat
     3241 ccttggatcc gggagataaa ccggtaaatg cgccattatc cggtgcaatt attcatgtta
     3301 atgtaaaacc cggcgaaaaa gtcaaatatg gacaagtctt gttaactctt gaagctttaa
     3361 aaatggagaa cgaaattgtc gctcctgaaa atggcacgat taaggcgatc tatgttcaag
     3421 ccggctccag tgttaatgtc ggagatgttt tgctaagttt agtaccatga ttgaattgag
     3481 tagacaagag gtgaatcaaa ttggcgcaac ccattaaaat aacagaaact gtttttcgtg
     3541 atgctcatca gtcattatgg gccactcgta tgaagaccga agacatgatg gctgttgccg
     3601 aggctattga cgagatcgga ttttactcct tggaggcttg gggaggagct accttcgaca
     3661 gctgtttacg gtttttaaaa gaagatccct gggtccgatt acggcgttta aaggctgtaa
     3721 tcaggaagac tcctcttcag atgctgcttc ggggtcaaaa cattttggga taccggcact
     3781 atccggatga tgtggttcgt gagttttgcc atcgggcggt tgataatggc attggtatca
     3841 ttcgaatttt tgatgcgtta aacgatatca ggaatatgga agtggcaatt aaggcttcca
     3901 aagaagcagg cgcccatgtt caaggaacgg ttgtttatac aatcagtccg gttcacaaca
     3961 ttgaatctta catcaaactg gctaaggaat tgactcagtt aggtgttgat tcaatctgca
     4021 ttaaggatat ggccggttta cttaagccat atgtggctga ggaattgatc aagggtctga
     4081 agaaggcggt taaagtcccg attcaacttc atggccatta taccagcggc ctggtatcta
     4141 tgacatacct taaagctgtt gaagccggag tagatgtagt agatacagct ctttccgcgt
     4201 tggcgatggg tacatcacaa ccggcaaccg aggcgattgt tgccgccctt caaggaaccg
     4261 agtttgacac gggactggat ttggctaaaa ttgctccgat taaccaacac tttaaggaga
     4321 tcaagaagaa ttatcaaaat gtctccgcac cggtggaagt tgatacggct gttttaaatt
     4381 atcaaattcc cggaggaatg attagtaatt tacgtaatca actggccgga caaaacgctg
     4441 cggaccgcta tggggatgta ttgaaagaat tacctcaagt ccgtaaggaa cttggatatc
     4501 cccctttggt tacacctacg agtcagatgt taggtacaca agcggtttta aatgtgatta
     4561 ccggaaaacg gtatagtgta gttccaaagg agattcgtga ttatgtcaag gggttatatg
     4621 gacggccgcc ggccccgatt gatccggaga tcaaaaaaat agttattggc gatgaagaac
     4681 caattaccca ccgccccgcg gatgatcttg aacccctgct tgataaggcc aggaaagaga
     4741 ttatgccata ctatcagcaa gaggaagatg ttctttcata tgtcctcttc ccggaggtag
     4801 ctatggagtt ttttaaattc cgggttggga ataaatgaac ttcataaaaa cgatgaagat
     4861 attttttaat tgacccttta ttattggagg cgtgttatgg agcaattaat aaatgagctg
     4921 ttcaagggag cgctttacct taattggcaa caactgatta tgatggccat tggtggaatc
     4981 ctcatttatt tggcgattgc caaggattat gagcctatgc ttttgctccc cataggattt
     5041 ggagctattc ttgccaatat tcccttttca tcagcaattg gtcatgatgg tttcttatcc
     5101 attctttaca atgcgggaat ttatactgag ttatttccgg tattgatatt tattgcagtg
     5161 ggggcaatga ttgacttcgg accgttgctt caaaatccat tcatgctttt ctttggcgct
     5221 gcggcccaat ttgggatctt tgccacgatg atggttgctt tgttaatagg atttgatctg
     5281 aaacaagcgg cttcaatcgg tattatcggg gcagcggatg gtccgacttc aatttatgtt
     5341 gcaacgaaat ttgccaaaga tcttctggcg cccatatcgg ttgccgctta ttcgtatatg
     5401 tctttggtgc cgttgattca gcctccggtc attaaattgt tgactaccaa aaaagagcgg
     5461 ttgatccgga tggaagcccg gcaagttaat atccccaaaa ttgtccggat tctctttcca
     5521 attctggtta cagtggtggc cgggattatc gctccgatta gcgtagccct ggttggcgcc
     5581 ctgatgttcg gcaatttaat ccgcgaatgc ggagtcgttg aacgtttatc caaatccgct
     5641 caaaatgaac tgtcgaattt ggtcaccctg ctgctcggaa ttacgatagg ctctacgatg
     5701 acggccagac aatttcttaa tcctgcaacg atcatgataa tggtcatggg tattgtcgct
     5761 tttatctttg atacggcagg cggagtaatt tttgccaaat tcttaaacat ttttctccgc
     5821 aaaaaagtca atccgatggt cggcgcagca ggaatttcgg cgtttcccat gtctgcccgg
     5881 gtaattcata aaatggccca aaaagaagac ccgaccaatt ttatcttgat gcaatccatc
     5941 agcgccaatg tagccggtca gttagggtcg atcgtagccg gcggactttt actcgcaatc
     6001 gttccggtac tattgaaata ggaggaaaac gagattgaat acggatctta ttaacaatgg
     6061 gcttattgct tcaggttttg gtttaggcgg ggtttttctg gttttgatca tattttatat
     6121 catgataaaa ataatgttat tcgcttttag aaatttcccg gataaataat ccggtttttg
     6181 acaatttgtt attttatcca aaacagggct ttcttgcgta attaaggaaa gcccttattt
     6241 ttttgcgtat gtttgtacat atgtagaacc agggtgttgt ataggtaaat tgtattactc
     6301 acaagtaaaa attcaaaaga aaactccagt gataaggtac tggagtttca cgaacatttc
     6361 ttcagattgc agaaaccata gtgaacgcga tgaccggcag taaagtttac ttaactatcc
     6421 gtaaataatt tgccagttgt ttaatagtaa ttgaattgca ggttatccgg acccgcttcg
     6481 gtatccgggg tatccgtaaa ggccaagaca ccctccatat tgaaaaaacc tttccggggc
     6541 gccacaaatc tggccgcttc cagggcaccc tcggcaaaaa ccgctcggga ataggatctg
     6601 tgggtaatct ccagctcgtc attgtcgccg gcgaagagga tccggtgtac accggcgata
     6661 cctcccgccc ggatggcatg gataccgatc tctcccgctt ccctcggtgt agcgcctttc
     6721 cggccgaagc agagtttctc ccgtcctttg atcccacggg ctgcattgac tgtggaggcg
     6781 atcttcaatg cggtacttga gggggcatcc tttttataac gatggtgcaa ctcggtgatc
     6841 tctatatcat actgcgggac gagcgctgct aaagtttgga ccaggatgag aagcaggttg
     6901 atccccaccg aaatgttggg ggcataaacc aatccgatat gatgacgccg tactaaacct
     6961 tgcaactcgt ggagctgcag cttattgaag cctgttgttc caatgaccag atgcacgtcg
     7021 tgttctgcta ggattcttgc gttgtgcagg gatgcctccg gcttggtgaa atcaatggct
     7081 accgtaaagg gtgttttacg acactcttca gccagggagc ttgcgtcgcg tatcacgagg
     7141 tccgtatcac agttgataaa ctcccgtaaa ctccggttag ccttggaact accggggctg
     7201 gtgataactg ccgccagttc cagatcatcc gtttttaaca gttgttccgc cactacgctt
     7261 ccggttctcc ccaaacccaa caaacatatt ttcattagag cacctcctga gaaaaaattg
     7321 atacgctaga gggtgaaact atctaccggg gataaaaaaa ggagggcata agaaaagacc
     7381 atggagggag ttccatggcc tttgaaacca ggccaaaact cgcctctctt gctacgcctg
     7441 cgaagttagc tgacgggttc ggatcgaaga gtgaccctaa aattgatgtt gaatgtgaat
     7501 ccggatttgg attcgaattc aacataattc attcaccccg aagactggtt cccccgttct
     7561 ccggcaagga ccggagattc ggcgttatag ggacgttcaa cattattcta tgtagttttg
     7621 tttattatat gtcacgacgg gtcttttcgt caaaccggac gtacggcttt gaggagcgta
     7681 tatcggagtt tttactgttc ggctaaatcg gtataactgg ttatttgtta ataagcggat
     7741 ctcagaggaa actgttttcg gggttttgtg aaatagagaa ggatagcgat aggattttgg
     7801 agggacggaa gcgaatatca aactgatgtt cgttagtgga aacgggaaaa acggttgata
     7861 agtcgtccgc gccaggtatt ccagacacca gaggctctcc gggaatgatc ctcggagaat
     7921 ccgggcagga agcagcataa cgccaccagt cggaccaggg cggagaggat gaataaacct
     7981 tggaagctac tgaacgactc ccccatcaga aaaggcaagg aatgtgacgt aaaaaacgcc
     8041 cgcgtgaact gcatgaaggc gccgccgcag ataaaagcca gtacgctccc ggcaagggag
     8101 gtaatcaggg cgtaggtggc gatgaaaacc gagcggttct gttccggcgc cagccaaatc
     8161 gaaagattgt tgagggtgac atccagcccc ggctgggaaa gaccgatcag agtatagatc
     8221 accggaataa gtaatccgta attgcctggt gttacgaaac accatataaa tggggtcatg
     8281 atattgatgg cgcagcagat ggccaccacc ggtttattac cgtaacggtc ggcaattcgc
     8341 ccccagacct ggatgaaaag gacggtggcc aggttgctga tgatctggga gagcagaaag
     8401 atcttgaaat agttcatttt cagggattcc agcatataga cgttgaaaaa gggtgcggca
     8461 aaattgaaac cgaaattcca gacggccaca aaacggatga acttcatata acgccggtcg
     8521 gtgaagggca atcggaaaag ctgcttaaaa gaggatcgtt gttgggaaag gttttggggc
     8581 ggagactcaa ctccgaaaaa ggatgtgata tcccctgcgc caaagagaga gccgattcca
     8641 aaaaggatgg cgaatccggt aaaacccggt acccgatcga ggaattttcc tgccgccagg
     8701 ccggccagag cgccgacgat ggtgtagatc atataccgcc tggcaaaaaa ccttccccgg
     8761 atgccgtcgg gaaccagacc ccccatccag gagttgaagg tcacgccgac catcgcattg
     8821 gttcccgccc agacagcgat cagggctgtc actatcgcca gtctttcggg acggtgcagg
     8881 cctggaatga agaagggtac tagggtaatg gggatccaga gcaaacggaa gaaaccggag
     8941 atcaaaaaga ccgatttcag atgaccgcgg gtctccagaa aataggcggt ggggatctgc
     9001 gcgatgttgc cgagcagcgg caaggccata atgatcccgt ataacaagtc gttcatgccc
     9061 aaagcccgga gataaccggt gagcggagct ccgttgatca tggtaaaaaa gacgatgccg
     9121 aaggagacac cggtgatgac cagggttaag ctgcgacgca gggcctgact cgtagaaaga
     9181 gcttgacggc tcataacaac ctcggatgaa aatacgtgaa gttacgcttg ttgtaataaa
     9241 gccattataa cataaaaaat cagacttgtt ggatgatttt ccaggaaatg aagggattgt
     9301 aatgaaccat cctgttttgg aacaatttca tccggcggtg gcaacctggt ttcaggaaca
     9361 gttccctgct cccaccccgc cccagataca aggctggccg ctcatcgcgg ccggtcaaaa
     9421 tgttctgatc ctagcgccta cgggctccgg taaaacgctg gccgcatttc tcaaatgtct
     9481 ggattggcta tatcaagagg ccgccgcagg ccataggatt gacgatggag ttaaagtcct
     9541 gtacatatcg ccgctaaagg ccttaaatca tgacattcac cgcaatttgg aactgccgct
     9601 atcggggatc cgtgaaaccg cagagcggat ggggatggat ctgccggtgc ttcagaccgc
     9661 agtccgtacc ggcgatacac catccgccga acgccagcgg atgctgcgcc ggcctccaca
     9721 gatcctgatc acgactcctg aatccctctt tttgctcctc tcttctcaag cacggaggat
     9781 tctgggtacg gtccgttacg tgatcgtcga cgagctgcat acggtttttg ccaataagcg
     9841 cggcgcccat ctggcccttt ccttggagcg cctggaacaa ctggccggtt ccagccagcc
     9901 gctggtgcgg atcgggctct cggcaaccat ccggccgctg gatgaggctg ccgccttcct
     9961 cggaggcaat tcatgggatg aagcaggtga gatcctgaaa ccccgtccgg tggcggtggt
    10021 ggatgccggt gaaaagaaga cttgggatct ccagatgata ctgcccctcc ccgatctgcg
    10081 ggaattaccg gagaagtcga tctggccgcc catctatcag aaattactcg atatggtgcg
    10141 agcccaccgg acgacgctgg tctttgtgaa caaccggcgg caggcggagc ggatcaccgc
    10201 caatctgaac gagctcgcag ggaaagagat tgcccggacg catcacggca gcatctcccg
    10261 ggaagtccgg ctggaggtgg agcggcttct aaaatcaggg gaactttcct gtattgttgc
    10321 caccgcttcc ctggaactgg ggattgatgt gggctcgatc gacctggtgg tccagattga
    10381 atctccgaaa gaggtctccc ggggcttgca gcgggtaggc cgtgccggcc acgtagtggg
    10441 attgcccagc aagggaagga ttatccccaa aacaagggct gacctgttgg aggcggcggc
    10501 gatcctccgc gaaatgcggg ccggacgggt tgaaaccagc aaggctccgc gcaattgcct
    10561 ggatgtgctc gcgcaacagc tggtggccat caccgctacc ggggagtctc cggtggatgg
    10621 ggtttaccat ctgacacgct gtgcatacaa ctatcgggat ctttcccgga gcgagtttga
    10681 aaatgttctg gcgatgctgt cggggagttt ttcaacccgg gaatacacgg aattaaaacc
    10741 caggctatac tggaacaagg acgccggagt catcggaccg gatccttatg gcaagcggtt
    10801 agtcttcagt tcagggggaa ccatcccgga tcggggatat ttcggggtat atttactggg
    10861 ttcgggggta cgcttgggtg agcttgacga ggagtttgtc tttgaacgga ggttaaacga
    10921 acggtttgtc ctgggaacct ccctctggaa gattgaggag atccgccagg accgggtgat
    10981 cgtcagcccc gcccggaagg gtgaggcgat cgttcctttt tggaaggcgg atcagggtgg
    11041 cagacccttt gaattgggac aacggatcgg agcttttttg gagtcagccg aagcacgact
    11101 ggaggcgccg gatttcatcg agtggttgga agaggagttt ggcctgaccc gggatgccgc
    11161 cttgaacctc cggcgttacc tgactgatca gaaacgggct ttaggctttt tacccactca
    11221 taaacgtttg gtggtggagg agtttcccga tgaagtaggc gagtggcggg tggtgattca
    11281 ttctcccttc ggattacggc tccatagtgc cctgggattg ttagtcagag aggaatggca
    11341 gcagcgacgg gggatcacgg tggaggagat ggcaagcgac gacgggatta tgttcgagtt
    11401 tcccgggggc gatgagccgc cccgcttgcc atgggaggag ctgccccggg aaggactgga
    11461 ggaacgtttg gcggggttgg tctccagtac accgttattt ggggctgtgt ttcgccaggc
    11521 ggctcagctt tcgttggtaa tgcccagggg cggttatggc cggaaacgca atccgctttg
    11581 gctcacgcgc ctgaaggcgg gaaacctgct tcaagtggta tccaagtacc gcgattttcc
    11641 attggtggtg gagacctatc gtcagatcct tcaggattac ttcgatatcg cggcgcttcg
    11701 ggaggttctg gccggattgg ccggcggcag tctggaggtt catacctgcc gacgcgatcg
    11761 gccttctccc ttcgcccagg ggtatttatt gaatttcgtg aacagtttca tgtatgagag
    11821 cgacaccccc aagggggaac ggcgcttgca actctttgga ttgggccgcg ataccttgcg
    11881 agcgctggtg ggcgccgagg ggttccggga gttgtttacc cccgaagcgg tgcaggcagt
    11941 cgccagaaaa gcgcagggac tcgaggttct ggaccgggag gtcgatacgg agcgggtacg
    12001 ctactggttg gaacgccggg gagatgtggg gacggaagaa ctggagaccc tttttcccga
    12061 aaacgcagtc cggattcggg aagaattaca gaggttggca gcaagcggcc aggccgtatg
    12121 gatacgccgc gatcccggcg gcccgcagct ggtgatggcc ggaacgctgc gcgatgaata
    12181 cttggctgct ctagatgacc tgaggcttga aaatccggtc gctctccggg ttaatgagga
    12241 ggaaacgaat cgtagtagta gggcgccatc cgctttaatg agtcgggagg aggcgcgccg
    12301 ccgtattgtc gaacggtatc tccggaccca tgggcccttt ggtgtagccg agatttgccg
    12361 gaggtacggt tttgccgcaa cagaggtcgc aacggagctt tcgatcttgg cagcggcggg
    12421 attggcggag ccgggagagt ttgttcccgg gggacgaggc gaggaatggt gcagcccgga
    12481 gcttttgagg gagattcacc ggcgtagcct ggctcaggcc agaaaggaga ttgaaccgcg
    12541 aaaacctaag gcctatgcgg tattggttgc cggatggcag ggggttggct gcaaacgggt
    12601 gggactggaa ggtttagccg aaacccttac tcagctggca ggggtttggt tacctgcggc
    12661 ggattgggaa ggcggagtgt tccccgtcag ggtgcgggat taccgtccga tcatcctgga
    12721 tcaactcatt gccagcggtc aaattggttg ggaggcgcgg ggagagggaa acaacctcaa
    12781 aatccggttt tacaatacaa cttcaattgt aatggctccg gatactattc ctgtgggtgc
    12841 cgggctcacg gaaccgggtg tttcaccccg gcaaagcgta ggtgagcttt cggcgggggc
    12901 ggtggaaatt ttagatctgc tccggaaccg gggcgcgctg acgctgccgc aaattctgca
    12961 agtcaagggc ggcggaacga tgactgtttg ggaagtcctg gaggaactcg tccgggccgg
    13021 ccgtatcagc aatgattctt tcggaccggt gcgatatctg ctggagaccg cggtcaagga
    13081 tcgaagcggc gcccgtggag tgcttcaacc ggcggtgctc gcccggatgg gccgttggtc
    13141 gctcctgccg gagcttggcg aaatggagag agaggtccgg gtggaagggt tgctcaaccg
    13201 ttacgggatg gtctgccggg agacggtcgg gccggaggaa ttgtcctggg gtgagcttta
    13261 tcccggtttt gagctgctgg agaatatcgg taaggcgcgg cgtggctatt ttgtggaggg
    13321 actgggcgga atccagtttg cctggaccgc cgctgtggag cggctacgca gggacccgga
    13381 accgggtaag tgttgggcgc tggggtggca tgatcccgcc aatgtggtgc ggatctttcc
    13441 ggagtggccc ggcgcggagg aacttttacg ggcgactccg gattgggtgg tctatgaggc
    13501 gggtgagccg gtgttagtgg cctcggggaa aaagctgaag ctgcaggcgg ttacggaaag
    13561 aacgccgcag tcgttgaccg gggcgttggg ggaactggtg cggctgttgg cgggggctta
    13621 tccggaccag aaaatcgcgg tggtgcagtg gaacggggag caggtgctag agagtccggc
    13681 ggaagaggtt ttggtggggg tggggtttga gagaggatac cgggagctga cgctgtggcc
    13741 gtcagaacgc aatttacagt tcaagtagtc taaaggcgac tcaccccctt gttccccctc
    13801 tctacaacaa acattgtaga gagggggaag gctcatgggt tacaataagc aaaatagatc
    13861 tcagggggtg
//
LOCUS       DASVMB010000244         7492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_545779,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000244 DASVMB010000000
VERSION     DASVMB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545779"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1500
                     /locus_tag="VF531_07165"
     CDS             127..1500
                     /locus_tag="VF531_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7713787.1"
                     /translation="MRQAENRLGFSYRSDLFSMKTNGWLRIGLLCLAFIGLNLVWSFP
                     SAAADEPSGTTQSGPWIDRTSASGMTILLQKTKSPVVEITLLLKSGSGLDPADKKGTA
                     LIMNNLVYLKLGNVKAKIGDVSVATYPDYSLISFKTSPGDAKKALTEVKELLSYPLYS
                     YDVITDLKGLYRTDVQGMPVFNRVYYDLTRELYGSNHPYNDWIDSDSLAAIEGKDVYR
                     WYRQTYQPGNAILSISGGVRQSIRDLEKFFAAMNTEAVDRRLMISPVTLEENLELSRE
                     ERNGRVTSVGIGFAAPRLTDPEYPAFRVIAYYLEDYLHYFEELRVKQGLIYAGFVLYD
                     YLDKPKAPSLTFLTVTDPELLPRVEAKTLEVLDRLRTAGMDPDQIQQVVQAMKRQNEA
                     KRSEGTGISVRNALSQYLQNRLVYDENLLPKLEKVQTQDIMNAATKYLTHYVRVAYVP
                     QEQAANF"
     gene            1940..2488
                     /locus_tag="VF531_07170"
     CDS             1940..2488
                     /locus_tag="VF531_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7713788.1"
                     /translation="MNETQKIFEKTAKLFCQSLVYQVIKPTLQELSKSELTEVQLSCI
                     RYVHTHSEPSVGAIAAGLSISNAAAAKLTDRLVKKHLLSREEDLRDRRVLKIKLTPDG
                     ARLLEEIDRIESQQFQTILSRMSSGEMEALQQGLNGFLRAALVDASQIDQICLHCGWD
                     HLLDCPGNLRYRELTGHDKTEV"
     gene            2641..3213
                     /locus_tag="VF531_07175"
     CDS             2641..3213
                     /locus_tag="VF531_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX7713789.1"
                     /translation="MNFKGSQTEKNLLTAFAGESQARNRYTYFASQARKENLIQIADI
                     FEETANQEKEHAKRFFSQLQGGEVEISATFPAGVIGNTVANLREAAGGENMEWSSIYP
                     GFAGTARGEGFEAIAVLFERVAVAEKQHEKRYRDLLENIEKDRVFKKDAPVTWRCRNC
                     GYIHIGTEAPQSCPACAHPQGYFELLGENW"
     gene            3257..3631
                     /locus_tag="VF531_07180"
     CDS             3257..3631
                     /locus_tag="VF531_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HEX7713790.1"
                     /translation="MTQKLAVYKCGVCGNIVEVVHAGGGELVCCGRPMQLVTENTVDA
                     SKEKHVPVITKIEGGYQVKVGSVTHPMEEKHYIEWIELLADGIAYRRFLKPGDAPEAV
                     FQVQATQVSAREYCNVHGLWKA"
     gene            3981..4406
                     /locus_tag="VF531_07185"
     CDS             3981..4406
                     /locus_tag="VF531_07185"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7713791.1"
                     /translation="MESNEKFEDTEAWGKARELAKIIYQATALGQLPQDRVLQEELRR
                     VTLAVMVHIARSFESVDEKEYFNSITLTAQFITELKTQLYVAKDIDLLTESQFEQLYV
                     LAMEIRQALKNIRKPGNIPNRPVGDYAPDYESRIRSFLK"
     gene            4430..5545
                     /locus_tag="VF531_07190"
     CDS             4430..5545
                     /locus_tag="VF531_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7713792.1"
                     /translation="MSISELQLLEELVNIDSGTGDQAGIAKVAGTVRRELEPLGFRFE
                     ERHAGDGSVHYYAARGRGERILLAAHLDTVFPQGTATARPFTIKGERAYGPGVSDCKS
                     GVVTILGALKQLGMESPSQCEIGCFFNSDEEISSPGSREILAQLAPGTRFALVVEPAE
                     GESITIGRKGIGRMELRVFGKAAHSGSNFQDGANAVLELAYKIIELQKLIDLEAGITL
                     NAGVISGGIRPNIIPDYAATEIDLRICRPEQEEPLLESLRRIAFHCTVPGTTARIDGK
                     ITRPPMPVTAANQRLYEALRRISRIQGFDLDAVQSGGGSDANLLAALGVATVDGVGPI
                     GGGHHSDTEYLELPSLKRRIELLAAFLRESPGIRLTE"
     gene            5636..6316
                     /locus_tag="VF531_07195"
     CDS             5636..6316
                     /locus_tag="VF531_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX7713793.1"
                     /translation="MGTIVNVIAVLIGGSLGLLFREHFPERIAQTALQVMGLFTIFVG
                     ISMALQGQEAILVLMSLAFGAMIGEWLNIEASLDKLGGWLEKRFRISQGSPAKGFIAA
                     SLVFCVGSMAIIGSIADGVKGDHSILFTKAMMDGIISIPFAAGMGIGVLGAAFSILIY
                     QGGLTLLAAQLQPIFSTAVIRELTSVGGVMVMGIGINILGLQKIRVGNFLPALVLIIG
                     FVYLKAIL"
     gene            6715..>7492
                     /locus_tag="VF531_07200"
     CDS             6715..>7492
                     /locus_tag="VF531_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX7713794.1"
                     /translation="MSHLSNATMFEQISLWVVLGIAILGLAYAFLLRSQVMRKDKGDA
                     KMQEVWNAIREGADAYLGRQLKTIVPLIAVLTVVLFLSVYIVPPSVEALERFKGYTVE
                     QVKLIIGIGRAIAFIMGAVFSLIVGQFGMRMAVQGNVRVSAAARTSFPEALKIAYRSG
                     TITGMLTDGLGLFGGTIIFIYFGMAAPDALLGFGFGGTLLALFMRVGGGIYTKAADVG
                     ADLVGKVEAGIPEDDPRNAAVVADLVGDNVGDCAGMAADIF"
BASE COUNT         1839 a         1655 c         2068 g         1930 t
ORIGIN      
        1 acgatatccc gctgttgaaa aagatcaaga ccaaggatct acaggctgtg cttgacaagt
       61 atttcaacca gcctccacgt tgctggatct tggtacacag taattaccag gatggaggtg
      121 acagagatgc gacaagccga aaatagattg ggtttttctt ataggtccga tttgttctca
      181 atgaaaacga atggttggct gcggatcggc ctcctgtgcc tggcatttat cggtttaaat
      241 ctggtatggt cttttccatc cgcagcagcg gatgaacctt ccggtacaac ccaatccgga
      301 ccgtggattg accgaacttc cgcaagcggg atgaccattt tactccaaaa gaccaaatcc
      361 ccggtggttg agattacctt actccttaag tccggcagtg gtctcgaccc ggccgacaaa
      421 aagggtacgg ccctcatcat gaataatttg gtttatctga agcttgggaa cgtcaaggca
      481 aagatcggcg atgtatcggt tgctacgtat cctgattatt cactgattag tttcaaaacc
      541 tctccggggg atgccaaaaa ggccttgacg gaggtgaagg agctactttc ctatccgctt
      601 tacagttatg atgtgatcac ggatctcaag ggtttatacc ggaccgacgt tcagggcatg
      661 cccgtattca accgggtgta ttacgacctt acccgtgagc tctatgggtc caaccatcct
      721 tataatgact ggattgactc cgactctttg gcagccatcg aaggcaaaga cgtataccgc
      781 tggtaccgcc agacctatca accgggtaac gcgatcctct cgatatcggg aggagtgcgt
      841 caatccatcc gggatctgga gaagtttttc gccgcaatga atacggaagc ggtggaccgc
      901 aggttaatga tctcccccgt cacgcttgaa gaaaacctgg agctttccag ggaggaacgg
      961 aacggccggg tgacttcggt cgggatcggg tttgccgctc cccgactcac tgatcccgag
     1021 tatcccgcct tccgagtgat cgcatattat cttgaagatt atttacacta ctttgaagag
     1081 cttagggtca agcaagggtt gatttacgcc ggctttgtcc tttacgatta cctggacaag
     1141 ccgaaggctc ccagtctcac atttctaacc gtgaccgatc cggaactgct gccccgggta
     1201 gaggctaaaa ccttggaggt tctggatcgt cttcgtaccg cagggatgga cccggatcaa
     1261 attcaacaag ttgtacaggc gatgaaacgg cagaatgaag cgaaacgtag tgaaggaacg
     1321 ggaatttcgg ttcgcaatgc cctcagccag tacctgcaga accggttggt ttatgacgag
     1381 aatcttttac cgaagcttga gaaggttcaa actcaggaca tcatgaacgc cgcaacaaaa
     1441 tatcttaccc attatgtgcg ggtggcttat gtaccccaag agcaggcggc gaatttctga
     1501 accctcgctt cttgcttcca cgctcgactg tttaaaacat ctgattaatt gtaacgccga
     1561 catcactgtc ggctttttcg tctgtttttt gagcttaatg atggacttct aaaccaattc
     1621 tacggaaata taggctatat caaggccagc taaattaacc aagaggccac ggtaatggag
     1681 gtggtggcta tagaaacgat aaagagaagg gatcttttaa ccacgaaagg cactaaacac
     1741 acgaaaatgg cttaaggaaa ccgatatagt tttcgtgaat ttcgtgtgtt tcggggttta
     1801 ggtccaggtc tgtactccgg atctttaggg aaaaaatgct tgacattaat aattaaccat
     1861 gttaaaattt attcaggttg aaggcaatcg cgccattgcg attaacccat ttcgatctca
     1921 agtaagggga gtctataaag tgaacgaaac gcagaagatc tttgaaaaaa ccgccaagtt
     1981 attctgccag agccttgtct accaggtgat taagcctaca ctacaagaac tttccaaatc
     2041 ggaacttacc gaagtacagc tttcctgtat ccgttatgtt catacccatt cagagccttc
     2101 cgtcggagcc atcgctgcgg gattatccat cagtaatgcc gcggccgcca agctaaccga
     2161 caggttggtt aagaaacatc tgctcagcag ggaagaggac ttgcgggacc ggcgggtttt
     2221 gaagatcaag ttgactccgg atggtgcccg gttacttgag gagattgatc ggatcgaatc
     2281 tcagcagttt caaacaattt taagtagaat gagttccggg gagatggagg cgttacagca
     2341 gggtttgaac ggttttctcc gtgccgcgct ggttgatgca agtcagatcg atcagatctg
     2401 cttgcattgc gggtgggatc atttgcttga ctgccctgga aacttgcgct accgggaatt
     2461 aaccggacac gataagaccg aagtataatt tttttgtagc aatagttaac catattaaat
     2521 ttatattagg ttatccaatt ttagaagagg gggaattgcc caaaaagaca aaagaatgtt
     2581 cccgagatgc aatgcatatc aaaatggtag tttaatacgg taaattagga ggttatgtcg
     2641 atgaatttca aaggaagcca aacggaaaag aatcttttaa ctgcatttgc cggggagtct
     2701 caagccagaa accgctatac ttatttcgcg agccaggccc ggaaagagaa tctaattcaa
     2761 atcgccgata tcttcgagga gaccgccaat caggaaaagg aacatgccaa gcggttcttt
     2821 tcgcagttgc aaggtgggga agtggagatt tctgccactt ttcccgcagg tgttattggt
     2881 aataccgtgg ccaatctccg ggaggcggcg ggtggagaaa acatggagtg gagcagtatc
     2941 tatcccgggt tcgccggaac cgcccggggg gaagggtttg aggccattgc agtacttttt
     3001 gaacgggtgg ctgtagcaga gaaacaacat gagaagcggt accgggatct actggagaac
     3061 attgaaaaag atcgggtttt taagaaggat gcacctgtaa cctggcgttg ccgtaattgc
     3121 ggttatatcc atatcggtac cgaagcgccg caatcttgtc cggcttgcgc ccatccgcaa
     3181 ggttattttg aattgttagg ggaaaactgg taattatatc gcgcgctgcg cgtatcaaat
     3241 gaggaggata cgaacaatga ctcagaaact ggcagtttat aagtgtgggg tatgcggcaa
     3301 tatcgtcgag gtcgttcatg cgggcggggg tgagttggtc tgttgtggtc ggccgatgca
     3361 actggtgact gaaaatacag tagatgcgtc gaaggagaaa catgtcccgg tcatcactaa
     3421 aattgaaggt gggtatcagg tgaaggtcgg cagtgtgacc catccgatgg aggagaaaca
     3481 ttacatcgag tggattgaac ttttggccga cgggattgct taccggcgtt ttttaaaacc
     3541 gggtgatgcc cccgaggctg tattccaggt acaggctacc caggtttcag ctcgtgagta
     3601 ttgcaatgtg catggccttt ggaaggcatg aaaagacggc aatctgttcc caccagaaga
     3661 taaagacccc cggctccgcc gggggatatt tattttttga gctcatctgg ctgtgatggg
     3721 ttgccgttgt gaatgaaaag ttaacctata cattagaaac aatccacccc gtcttggaac
     3781 gggtggttgt tgcgttcgga cccggggtac agcaggaaaa aggaaaaata gcacgaaatt
     3841 aattcagaat aaataggtta aggataatgg gttcctcggc aagtctcgga ccggtgaatg
     3901 ggtcaaaaac tcgtggagaa acttgtatga gttaaatgtg gtatgaaagc agctgggttt
     3961 cgataaagga ggttagtcga ttggaaagta atgagaagtt tgaggatact gaggcatggg
     4021 gtaaagcccg ggaattggcg aaaataatct accaggccac tgctcttggt cagttgcctc
     4081 aggatcgggt tttgcaggaa gagttgcggc gagttacttt ggcggttatg gtccatattg
     4141 cccggagctt cgaatctgtc gatgaaaagg agtattttaa ctccataact ctcaccgccc
     4201 aattcatcac ggaactaaag acccagctgt atgtggctaa ggatatcgat ttgcttactg
     4261 agtcccagtt tgagcaactt tatgttctgg ccatggagat ccggcaagct ttgaaaaata
     4321 tcaggaaacc cgggaatatt ccaaacaggc cggtcgggga ttatgccccc gactatgagt
     4381 caaggatacg aagttttctc aaataaatat ggattggtga ggttgagcca tgagcatttc
     4441 cgaactgcaa ctcctggagg aactggttaa tattgattcc gggaccggag atcaggcggg
     4501 aatcgccaaa gtcgccggta cagtacgacg ggagctggaa ccactcggtt ttagattcga
     4561 ggagcgtcat gccggggacg gttcggttca ttattatgct gcccggggac ggggggagcg
     4621 aatcctgctc gccgcgcacc tggatacggt tttcccgcaa ggcaccgcca cggcccggcc
     4681 gttcaccatc aagggggagc gggcttacgg tccgggtgtc tccgattgta aatccggtgt
     4741 ggtcacgatt ctgggggctt tgaaacagtt gggaatggag tctccttctc aatgtgagat
     4801 cggatgtttc ttcaatagtg atgaggagat tagttccccc ggatcccggg agattttggc
     4861 ccaattagcc ccgggaaccc gatttgccct ggtggtggag ccggccgaag gagagagcat
     4921 cacaataggc cgcaagggca tcggccgtat ggagttaaga gtcttcggaa aggcggccca
     4981 ttccggatct aacttccagg atggggccaa tgccgtgttg gagttggctt acaagattat
     5041 cgaacttcag aaattgattg acctggaagc ggggatcacc ttgaacgccg gagtcatcag
     5101 tggcggtatt cgccccaata ttattcccga ttatgccgct accgagatcg atttacggat
     5161 ctgccgcccc gaacaggaag agcccctgct tgaaagtttg cggcggattg cttttcattg
     5221 tacggttccc gggactacgg cccgaatcga cgggaagatt acccggccgc cgatgccggt
     5281 aaccgctgcg aatcagcgct tgtatgaggc attacggcgg atctcccgga tccagggttt
     5341 cgatctggat gcagttcaat cgggaggggg ttcggatgcc aatctccttg ccgcgctcgg
     5401 tgtggctaca gtcgacgggg tgggcccgat cggcggtggt catcactcag ataccgagta
     5461 tttggaactc cccagtctca agcgccggat tgaactgttg gccgcatttc tgcgagaatc
     5521 tccggggatc cggcttacgg aatagccgta ggcggtatag ggggatttta ggattaccgc
     5581 attcgacttc tcctccaaaa actatcggta catttttccg gaatggagtt aatctatggg
     5641 gacaattgtg aatgtgatcg ccgttttaat cgggggttct ttggggttac tcttcagaga
     5701 gcattttccg gagcggattg cccaaacggc cttacaggtg atggggctct ttacgatatt
     5761 cgtcggaatc agcatggcgc ttcaaggcca agaagcgatc ctggtattaa tgagcctggc
     5821 tttcggggcg atgattggcg agtggttaaa tatcgaggcg agcctggata agctcggcgg
     5881 atggctggag aaaagattcc gtatcagcca gggaagcccg gctaaaggat ttatcgccgc
     5941 cagtttggtc ttttgtgtgg gatcaatggc gattatcggg agtatcgccg atggagtgaa
     6001 gggtgaccat tccattctat tcaccaaggc gatgatggac gggatcattt caatcccatt
     6061 tgcggcggga atgggtatcg gcgtcttggg agcagctttt tccatcttga tttatcaagg
     6121 cgggttaacg ttacttgccg cacaattgca accgatattt agcaccgcag tcattcggga
     6181 attaacctcg gtaggcgggg taatggttat gggcattggc atcaacatat tggggcttca
     6241 gaagatccgg gtgggaaatt tcttacctgc cttggttttg ataatcggat tcgtctattt
     6301 gaaggcgatc ctttaattca acagggagct tattgcgggg gttgttcgaa atattttgta
     6361 tctatccggg aaaaaaaggg tgatctttta tttacccggt ctttatgata agatattatg
     6421 ggcgataagt tgagttttta tacatcgtcc gggctatgtt attattatca atatgatgct
     6481 cttttgttct acattatctt acgggttggt caatcggggt gtttacgcat ccaaaggtcg
     6541 ggtttcaaaa cggtccgaaa atgaccgtgt cacatattac cccgtaacgt gtttttccag
     6601 cccggtttaa agggggtaga tgaagccaca agttgttaag gttcgttttg gttgatcccc
     6661 aatttttact tgggaaatat tatttcagaa cattaagaga aaggaacgaa cgaaatgtca
     6721 catttgagca atgctacgat gtttgaacag atctctttgt gggtcgtttt aggcatcgca
     6781 attcttggtt tggcatatgc tttcttactc cgctctcagg tgatgcgtaa ggataaaggc
     6841 gatgccaaga tgcaagaggt ttggaatgcg atccgcgaag gagccgatgc ttaccttggc
     6901 cggcagctaa agaccattgt acccttgatc gctgttttaa ctgtagtgct tttcctttcg
     6961 gtttatatcg tacccccgag tgtcgaggct ttggagcggt ttaaaggcta caccgtcgaa
     7021 caagtcaagt taatcatcgg tatcggccgg gcgatcgctt tcatcatggg cgctgtgttc
     7081 tccttgattg tcggtcaatt cggcatgcgg atggctgttc aaggtaacgt ccgtgtttcc
     7141 gccgctgccc ggaccagttt ccctgaggcc ctaaagattg cctaccgctc cgggacaatc
     7201 accgggatgc tcaccgatgg gctgggtctt ttcggcggta ccatcatctt catctacttc
     7261 ggtatggctg ctcccgacgc cttgttgggc ttcggtttcg gcggtacctt gctggccctg
     7321 ttcatgcggg tgggcggcgg tatttatacc aaggccgctg atgtcggcgc cgacctggtt
     7381 ggtaaggtcg aggccggaat cccggaggat gatccccgga acgccgctgt cgtagccgac
     7441 ctggtaggcg ataacgtcgg cgactgcgcc ggtatggccg cggatatctt cg
//
LOCUS       DASVMB010000245         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_472492,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000245 DASVMB010000000
VERSION     DASVMB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472492"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..758
                     /locus_tag="VF531_07205"
     CDS             471..758
                     /locus_tag="VF531_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HEX7713795.1"
                     /translation="MAKITKVPFSHKVAAFLDIPLDTVVDWPRVILSGNQSLVIQNHR
                     GVIEYDRSIARINTKLGELVVSGSGLTLVSAVKEEIVIEGKVERVELVDWR"
     gene            771..1979
                     /locus_tag="VF531_07210"
     CDS             771..1979
                     /locus_tag="VF531_07210"
                     /inference="COORDINATES: protein motif:HMM:NF018591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HEX7713796.1"
                     /translation="MGFTRLISWATGYVEIVVRGAHLEKLINLLTGSGLFVWDIKRIG
                     GDALVAKIRAHGFLRIREPLRRSGCRGRIRAKKGWPFLWRQIKRRKVFLLGAAFAVML
                     LIYLSSFIFFIKVEGFEGKDREILLASLEKRGVKAGLLRKDLLERKSRIEREIMVDLP
                     QAVWLGISVRGVVAEVRVVHRKTAPKTINICDIVAARNGVISKLIVIRGTPVVKEGET
                     VARGDLLISGTVWHGDPEHQEFSKEEVPANGVVEARVWYDFDVLEPRTVWQPVIGKTG
                     REEYSIRLGKKLHPLIGLGERPGKNYSWTRWHKRIYQGRNPVDVVEFIKDTWREVEWR
                     KVTRPLPKVKRRALTEATERLRRLLGKGPLVTKALSWSNEGDFVRLHVTVESVRDIGM
                     IALRQIPPGK"
     gene            2119..3102
                     /locus_tag="VF531_07215"
     CDS             2119..3102
                     /locus_tag="VF531_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7713797.1"
                     /translation="MEQKELKLEIEPHLVQELSGRFGEHFHQIETGLDIHIFSRGNEL
                     ELSGPASQVDRGAALIELMNAQIKKGHPPAVSELEYMIRLAGEDQLQKVAGLTEQVVA
                     STARGKKIYPRTIGQKLYVDSIHNNGLTFGIGPAGTGKTYLAVALAVAALQAKEVNRM
                     ILTRPAVEAGEKLGFLPGDLQEKVDPYLRPLYDALYDVMGVDLFQKYLERGTIEIAPL
                     AYMRGRTLDDSFIILDEAQNTTPEQMKMFLTRMGFGSKAVVTGDITQVDLPKGTVSGL
                     AEVGQIFHDVPGISVITLTDQDVVRHELVQRIIRAYERYQHEMEGANLGTQ"
     gene            3089..>3339
                     /locus_tag="VF531_07220"
     CDS             3089..>3339
                     /locus_tag="VF531_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713798.1"
                     /translation="MELNESQGLNPKKSKHPNPQSVEVATKLIGIFKMPSFREGLLIL
                     FCFGMLIILLQMNLFPRTLDAKVGQASKEDVITPRDIVDR"
BASE COUNT          824 a          753 c          978 g          784 t
ORIGIN      
        1 gaatatctat aacaatctaa atgccccggc ggctccggtc attgtaaagg cgatttaggg
       61 gatgcggcag taccgagatc cggcggcttt tgtgtgatta taggggggag cgggtataat
      121 cgagaagata accaacatca acggcttgac cttaaaaaat atcgatctat acggtaaatt
      181 ggcagaactt gaggaaagga ttttcaaatt ggaagcggag cggcgagagt atccgcctcc
      241 ccaaactatt attgtcgaaa aactctatgt ccgaagggtt gaagtggata aatttcagtt
      301 taaactggat gacattactg tcgacgactt gagcggtatg ttgaatgtgg gaattaacag
      361 cgacggaaaa atgaaaaagg aagaaaagaa ataaatacgc cgggtgtcaa cccccggcga
      421 ttcttctaaa acctcctttg attcgcatac aacttatagg agggattttt ttggccaaaa
      481 tcacgaaggt tccgttcagt cataaagtgg ccgcctttct cgacatacct ttggatacgg
      541 ttgttgactg gccccgggtg atcctgagcg gcaatcaaag cctggtgatt cagaatcacc
      601 gcggtgtgat tgagtatgac cggagcatcg cccggattaa caccaaactc ggggaattag
      661 tggtctccgg tagcgggctt acattggtga gcgcggtgaa agaagagatt gtcatcgagg
      721 gcaaagtgga gcgggtggag ttggttgact ggaggtaggg ggtaacggac ttgggattta
      781 cgcggttgat ctcatgggca acggggtatg tggagatcgt ggtccgggga gctcatctcg
      841 aaaagctgat taatcttctc accggatccg gcttgtttgt ttgggatatc aagcggatcg
      901 gcggcgatgc cttggtcgca aagatccggg ctcacggctt tttgcgaatt cgggaaccgc
      961 tgcgccgttc gggatgcagg ggccggattc gggcgaaaaa gggttggccg tttctttggc
     1021 gccaaatcaa gcgccgcaag gtgttcttat taggtgcggc ctttgcggtg atgctgttga
     1081 tctatctttc ttcgtttatt ttcttcataa aagtggaagg ctttgaaggc aaagaccgtg
     1141 agattctttt ggcttccttg gagaaacgcg gggtaaaagc cggtctgctc cggaaggatc
     1201 ttttggaacg taagagccgg attgaacggg agatcatggt ggacctcccg caagcggttt
     1261 ggcttggaat atcggttcgc ggtgtggtgg ccgaggtccg ggtggtacat cgcaagacag
     1321 cacctaaaac gatcaacatt tgtgacatcg tggcagcccg gaatggtgta atctccaaat
     1381 tgatcgtcat tcggggcacg ccggttgtga aagagggaga aaccgtggcc aggggagatt
     1441 tgttaatcag cggtacggta tggcacggtg atcctgagca ccaggagttc tccaaagagg
     1501 aggttcccgc aaacggcgtg gtggaggcga gagtctggta tgattttgat gttttagaac
     1561 cacgaaccgt ttggcagccc gtgattggga aaaccggccg ggaagaatat tccatccgtc
     1621 ttggcaaaaa acttcacccg ctgattggtt tgggggaacg acctggtaaa aattattcct
     1681 ggacacggtg gcacaagcgg atttatcaag gaaggaatcc ggtagatgtt gtagaattca
     1741 ttaaagatac ctggcgcgaa gtcgaatggc gcaaggttac ccggccctta cctaaggtga
     1801 aacggcgggc gcttaccgaa gccacggaac gcttgcgccg gttgctggga aagggaccgc
     1861 tggttaccaa ggctttatcc tggtctaacg agggagattt cgtcagatta catgttaccg
     1921 ttgagtcggt gcgcgatata ggaatgatag cactacgcca aatacctccg ggaaaatgag
     1981 tggctgcggt acgctccgcg ggagggcatc cgcatcgtct gagcctctca gagacaactc
     2041 ccgggatcac ggacaggctt tccgacttga atgggatcca gtggagtttc acattttgaa
     2101 agggcggcgc cagtttaatt ggaacaaaaa gagttgaaat tggagattga accccatttg
     2161 gtacaggagt taagcgggag gttcggtgag cattttcacc agatcgaaac cggattggat
     2221 atccatatct tttcccgggg caacgagttg gaactctcag gacccgcttc ccaggtagat
     2281 aggggcgcgg cacttattga attgatgaat gcccagatca aaaagggcca tcctccggcg
     2341 gtctcggaac tggaatatat gatccgcttg gccggggagg atcaattgca aaaagtggcc
     2401 ggactgaccg aacaggtagt tgcgtctacc gcccgtggta aaaagattta cccacggacc
     2461 atcggccaga aattgtatgt ggattccatt cacaacaacg gcctgacctt cgggatcggc
     2521 ccggctggaa ccggcaaaac ttacttggct gtggcccttg cggtagccgc cctgcaggcc
     2581 aaggaagtca accgcatgat tctaacccgg ccggctgtgg aagccggtga aaaactgggt
     2641 tttctccccg gcgatcttca ggaaaaggtc gatccgtatt tgcgcccgct ttacgatgcc
     2701 ttatacgacg tgatgggagt tgacctcttc cagaaatacc tggaacgggg gaccatcgag
     2761 atcgcgccgc tcgcttatat gcgcggccgc accctggatg actcctttat catattggat
     2821 gaagcccaaa acaccacccc cgaacagatg aagatgtttc tgacccgaat gggttttggc
     2881 tcaaaagccg tggttaccgg ggatatcacc caggtcgacc tgcccaaggg gaccgtttcc
     2941 ggtctggccg aggtgggaca gatcttccac gatgtaccgg gaatttccgt catcacctta
     3001 accgatcagg atgtcgtccg ccacgagctg gtgcaacgga tcattcgggc ttatgagcgc
     3061 taccagcatg agatggaagg tgctaatctt ggaactcaat gagagtcagg gtttaaatcc
     3121 gaagaaatcg aagcacccca atccgcagtc ggtggaggtt gccacaaaac tgatcgggat
     3181 cttcaagatg ccgtctttcc gggaaggctt attgatttta ttctgttttg ggatgctcat
     3241 tatcttgctc cagatgaatc tcttcccccg taccctggat gcaaaggtgg gacaagccag
     3301 taaagaggat gtcatcactc ccagggatat cgtggaccg
//
LOCUS       DASVMB010000246        13420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_545996,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000246 DASVMB010000000
VERSION     DASVMB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13420
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545996"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF531_07225"
     CDS             <1..442
                     /locus_tag="VF531_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7713799.1"
                     /translation="DRVVAVYIIGQVILASVLGVVLYIGYLILGLHYPLILALFALVA
                     AFIPMFGPIIGVVPAVLVGLAQNPWLALEIVILAVGVHLLESHLLAPQLIGKRLAIHP
                     LTIIFIFLVAAALFGFVGVLVAVPVYAVAKIIFKGVIKIVKSKV"
     gene            complement(466..1857)
                     /locus_tag="VF531_07230"
     CDS             complement(466..1857)
                     /locus_tag="VF531_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7713800.1"
                     /translation="MQTQLTEKRQLRREIIDLTLPVLAEQSFITLMGVINAIMAGHIG
                     KEATAAIGMVDMLNNIFIAIFSALAVGGTVIVAQFTGQTNYRRANESAKQALYAGVIL
                     AIVITFIIGLFRQPLMGLLYGAADRRVLSYALPYLEITLSTYPLIAFTSIACGVLRGA
                     GDTKSPARIVILMNIINICSSYLLIFGISLGNLVISGLGVRGAALGIGIARLMGALAL
                     LYIIVRGSLNLKVYHIFRFKPDWELLRAVLNIGIPASIETLFFNAGKLITQTFIVAMG
                     TVAIVSNYVASSLHSLMTIPGSALSTVSTAIVGQYMGRSDKAGAKKILFYMNKLGTVS
                     LMAMNLLFLPLFPLFASLYTPNHEVIRQVTVLLQYTALFLVFWPISFVTPSGLKGAGD
                     VRYTLIVSAISMWAFRITLGYILCIHLRMGILGVWLGMYTDWIIRGIAFILRMKGNNW
                     QKHVIVGHASQGQ"
     gene            2203..3537
                     /locus_tag="VF531_07235"
     CDS             2203..3537
                     /locus_tag="VF531_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7713801.1"
                     /translation="MEPIQSSLFEGMNPKAPLPDRIRPRNLDDFVGQEHLLGSGKVLR
                     RLIEQDKLSSMILWGPPGVGKTTLAMVIAAMTHAEFVTFSAVTSGIKEIREVMSQAEA
                     SRSLGRRTILFIDEIHRFNKAQQDAFLPHVEKGNIILIGATTENPSFEVNSALLSRSR
                     VFVLHALKTEEIVELLKNALRDERGFGGMSIAIDPDLLERIAVFANGDARAALNVLEM
                     AVNSAPQVHGSVTIDAAIVADSLQHKTLLYDKKGEEHYNIISALHKSMRNSDCDAAVY
                     WLARMLEGGEDPLYVARRLIRFASEDVGLADPRALSIANAAYEAAHYLGMPECNVNLT
                     EAVVYLALAPKSNALYLAYESAKIDAAQTLAEGVPLWLRNAPTKLMAEVGYGKGYRYA
                     HEFAAKTTNMQCLPDNLREKRYYHPTEQGLEKNFAERLALIRKLKEKDPAEI"
     gene            3906..6395
                     /locus_tag="VF531_07240"
     CDS             3906..6395
                     /locus_tag="VF531_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX7713802.1"
                     /translation="MAIRKRVLYVVGTACTILIVLLYSTTQIFLASRFGAIERDSVHQ
                     SLLQVVNALDNELHDLGKETDDWASWDDTYRFIATGDPNYISINLPDETFMDLQLNLI
                     LYLDKSNRVVYGKAFDLTKAREIRDPRIFTILALSDRLTNVKGEQSRTGIVLLPEGPL
                     LLAAHAILTSERKGPSRGSLMMGRFLNQDEIRKLATITHLPIAIYPYVQFQNRFQEKR
                     FHLDLDHPKLVQPLNGHQVLGGRLLSDLSGNPVLVATVQLPRNIYRQGQASIFYFGLS
                     LVGVGVFLAILVAGFLGKVVLNRLASLNQCIKQIAISSDLSIRLPVSGADELSNFARV
                     LNRMMNNLEQMHQKLEDSNRQLAAIIEFLPDATLVIDEAGRVVAWNRAMEEMTGVPKQ
                     EIIGRGDYAYAIPFYGKPRPLLIDLVQSDPGEIKRYYEHTEKKGSVLYSEVYVDTIGK
                     KQGGYLWITASPLVDQEGRLVRAIESMRDITKRKKIEEELKYLSLHDPLTGLYDRAYF
                     EEEIRRLERGREPVVGVIMCDVDGLKLVNDSLGHSTGDKLLQTAANVIRSAFRQNDVV
                     ARVGGDEFAVLLPQCDGTLLETAGRRVQESVENHNRSNPELPLSISLGWAFRKEASVR
                     LKDVFEEADNNMYREKLQHHQSNRSAIVQTLMNTLRARDFVTEDHADRLKKLVADLSR
                     KLGLDEQKIHDISLLAQFHDIGKVGIPDQILFKQGPLTQEEWKIMRRHTEIGYSIAHS
                     AQELMHISDWILMHHERWDGGGYPRGLKGDAIPLECRILSIADSYDAMTSDRPYRKAL
                     THSQAVQELRKCAGTQFEPKLVEEFIEMIGE"
     gene            6673..7689
                     /locus_tag="VF531_07245"
     CDS             6673..7689
                     /locus_tag="VF531_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEX7713803.1"
                     /translation="MSEHEITVSHMVLHILDPEAGVPVLSNREIDPSEDGFDFTEKLI
                     RKMLDDENLKNAVFFDGPNRTAELCASLFSGATGFLDATRDLAGGLFEIMSQHPEIPA
                     ADLICCLAEIDGVPYLGLLKLNYRSNFTHQVQYEGNAPVNLLFRQRAVLPGENQKPDE
                     GVLINLQDRTLRLVEKQHELDGEKEYYLSKRFLGCGSQLSSAEKAKILNRVAVKLGKK
                     YNNEKLDSSARLRKSVTEHLETGGEVALTTVAQEIFRDAPTAQEEYVSELKEAGIREE
                     KVQFSEKLAARKFKNQKIKTDTGVEIEFPATHYMNKDLIEFVPQPNGTLSIIIKNVGK
                     ITDR"
     gene            7720..8202
                     /locus_tag="VF531_07250"
     CDS             7720..8202
                     /locus_tag="VF531_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7713804.1"
                     /translation="MNSYHMRRKDRQITDPEMIGGVLRSGKYAVIALCRENEPYVVTL
                     SYGYDEAGKALYFHCAPSGLKLEFIQANPCVCATVIQDQGYVPGQCEHRYISLVLRGR
                     MTIINDLPGKKHGLDILLAHLEDDPAPILARNIKDQASYDRFVMLRMDITECSAKQGS
                     "
     gene            8225..10171
                     /locus_tag="VF531_07255"
     CDS             8225..10171
                     /locus_tag="VF531_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7713805.1"
                     /translation="MNLLAAEDISKSYGTKALFSGLNLGVNSGDKVGLIGVNGTGKST
                     LLKILMGIEPPDSGRIIHATNARIGYLQQNPVFKERSTVIQAALQGNSPQMQLVREYE
                     SALAEAEAAPDDHKIRARLATLGERMDEAGLWQMESQAKMVLTKLGISDFSAVVDTLS
                     GGERKRIALAAALINPVDLLILDEPTNQIDADTVAWLETFLKERQEALLMVTHDRYFL
                     DRVTNRIVELDRGQLYSYSGNYSLFLEKKAEREEQEQATAAKRQNLFRRELAWIRRGA
                     KARSTKQQARIDRFQKLETEITTTGPAREMEITAGASRLGKKVVILEQLGKSWDGRPI
                     ITDLDYTFLPGDRVGIIGPNGVGKSTLLRLIAGMIQPDTGRVEIGSTVKFGFFTQEAL
                     KGDESQRVIDYLKEQAEYLAIADGQRISASQMLERFLFSPDMQWTPIAKLSGGEKRRL
                     FLLRVLMTAPNVLLLDEPTNDLDTQTLGILEEYLDGYPGVVITVSHDRYFLDRVVEKL
                     IALEPDGSIRHFTGNYSDFLEERFEKSGLSSSGKNRELPAPEPKLPEKTAHPKPQLKL
                     SYKEQRELDGIEAVIDATEGRLARVRERIAQAGSDYQALQELIREEQDTDHELQELMN
                     RWVYLNELAAEVARNKSSKTNSGS"
     gene            10260..10883
                     /locus_tag="VF531_07260"
     CDS             10260..10883
                     /locus_tag="VF531_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX7713806.1"
                     /translation="MNTVQVSFDYPERLSRGILLLKTFLGFIILIPHLIALFALGIAA
                     GLVMFISWFAVLFTGKYPQSLFDFEVGVQDWGLRVSAYMGLLRDEYPVFGLEARYPSR
                     FQVHYPDKLSRGILLLRLFFGWIYVMIPHGVCLLFRGIAYLAVTFVAWWAVLFTGKMP
                     RRMFNFLAGTLRWNQNLNAYLMFLTDEYPPFTGKENGSGSAGTGFIA"
     gene            11118..12389
                     /locus_tag="VF531_07265"
     CDS             11118..12389
                     /locus_tag="VF531_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713807.1"
                     /translation="MFKFATVQIRLGKSFRALGHRDFRYFWTGQCISLLGTWMQRTAQ
                     QWLVYSLTKSPFLLGILGVFQFGPMLLFSLFAGTLVDRFPKRRVLIFTQTVLMIQALI
                     LALLVWTGQIQYWQILILALVMGFVNTLDMPTRQSYFIELVGKDDLMNAIALNSAIVN
                     IAKIVGPAIAGIVMLYLGTAICFFLNGISFIAVLIGLFLIRTSDTRLRAQNTKLLADV
                     GEGLKYIASKPVLWSTVLAMFAVGTFAMNMDVLIPVFAGEVLHRQASGYGFLLAAMGI
                     GSLVGALLVSTRSRRGLSAKILFGSAVIECLFYLVLGFVHHYELAMFFIAVIGFFSII
                     FMTTANSTVQLNSDDGHRGRVMSVYSLAFAGTTPIGNFFAGSVAEKMGGNFSFVACGA
                     VTLACIVWILLKTKRHVNLVSGDFHNPDNLV"
     gene            complement(12419..13258)
                     /locus_tag="VF531_07270"
     CDS             complement(12419..13258)
                     /locus_tag="VF531_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713808.1"
                     /translation="MLFRTVIALGEAVLGYFCTSLGFVLQKKGIGWLGFQGRKGPGYY
                     RDLWTWGIGFALLNLAVLPNYLSLGVLNAYIVNAISGLNIVFMIFLSRWILNEPLHPQ
                     DYLYTLIMCAAIVGINLADGSGASVSTIQADFAYLAAAIPVGSLFIWFLLRVAKVLRP
                     TSGLQALWLAAASGSMSGLMITYMKILQLERGTQLLSYLGSPYLYCFFTVSALSMVAI
                     QMAYRMGSMILVGPAQFATTVFYPILAAYPIFSLPVNPVQVVCFIAIVTTVVLMVKDH
                     ARE"
BASE COUNT         3389 a         3115 c         3612 g         3304 t
ORIGIN      
        1 cgatagggtg gtggcggttt atattatcgg ccaggtgatt ctggcgtcag tcttgggagt
       61 cgttctctat atcggttatt taattctcgg gctgcattat ccgttgattc tggctttgtt
      121 tgcgttggtg gctgctttta ttccgatgtt cggcccgatt atcggagtgg ttcccgcagt
      181 ccttgtcggc ctggcccaga atccgtggtt ggcattagag atagttatcc ttgcggttgg
      241 tgtacatctg ctggaaagcc atctactcgc cccccaactc atcgggaaac gactcgccat
      301 ccatccgctt acgattattt tcatattttt agtggctgcg gcccttttcg gctttgtggg
      361 agtgctggta gcggttccag tctatgcggt agcaaaaatt atcttcaagg gagtaatcaa
      421 aattgtgaag agtaaagttt gaagttagaa ataaaaccat aagccttatt ggccttgact
      481 ggcatggcct acaataacat gcttttgcca attatttccc ttcattcgaa gtataaacgc
      541 gattccacga atgatccaat cggtatacat cccaagccaa acccccaaga tccccattcg
      601 tagatgaatg cacaaaatat agcccagcgt gatccggaag gcccacatac tgatggctga
      661 gacgatcaaa gtatagcgga catcgccggc gcctttcaat ccggagggtg tcacaaagga
      721 tatcggccaa aaaacgagga acaatgcggt atattgaagt aacaccgtaa cctggcgaat
      781 gacctcatga ttgggtgtat ataacgaggc gaatagggga aatagcggaa ggaaaagtag
      841 attcatcgcc atcaggctga cagtacccag tttattcatg taaaaaagga tttttttagc
      901 cccggcttta tcggatcggc ccatgtactg tccgacgatg gcggtggaga cggtacttaa
      961 ggcactgccc ggaatggtca tcagactgtg cagtgaactg gccacgtaat tggagacgat
     1021 ggccacggtc cccatggcga caataaatgt ctgagtgatt aatttgccgg cattaaagaa
     1081 taacgtttcg atacttgccg ggattccgat gttcaaaacc gccctgagca gttcccaatc
     1141 aggtttgaat cggaaaatgt ggtaaacctt gagattcagg gagccccgga cgataatata
     1201 cagaagagcc aaggcgccca tgaggcgcgc aatcccgatc cccagtgccg ctccccggac
     1261 tcccagaccg gaaatgacca ggttgcctag ggagatgccg aatataagca agtaactgga
     1321 gcagatattg atgatattca tcaaaataac gattcgggcc ggtgatttgg tatctcccgc
     1381 accccgcaga acaccgcaag cgattgaggt aaaggcgatc agcggatagg tagacaaggt
     1441 gatttccaag tagggaaggg cgtagctcag tacccggcga tcggcggcgc cgtataaaag
     1501 tcccatcaaa ggctggcgga atagaccaat gatgaaggtg atcacgattg ccaggataac
     1561 tccggcatat aaggcttgct tggcggattc attggcgcga cggtaattgg tctgtccggt
     1621 aaactgcgcg acaattaccg ttccaccaac ggccaatgcg gagaagatcg cgataaaaat
     1681 attgtttagc atatcgacca tgccgattgc cgcagtggct tcttttccga tatgtccggc
     1741 catgatggcg ttgatcacgc ccatcagggt aataaacgac tgttccgcca gaacgggaag
     1801 ggtgagatca ataatctccc gtcgtaactg tcttttttcg gttaattgtg tttgcatgac
     1861 ctataccggg tgattctttc tttgataata ttgaaatcgg ttcatgagtg tccctagata
     1921 agtggaagaa tttaactcaa acattgatct atgaaagctt ttgaaaaagg ataattccat
     1981 taaacacccg gtgatgccgg aagtcaaatc cagtgtaccg gaagatacgg caagtttcaa
     2041 cctatcggta aaaaatttaa cctttccgcc gtcacttcta atgaatcacg ctcgggaaag
     2101 cggattgcca tcgtttggca agagggttat aataaaatca gtctttggat gggtattaag
     2161 gcagcgcggc cagccaacga gggatgaagg tgaggcaagt ttatggaacc gatacagagt
     2221 tcgctgtttg agggtatgaa tccgaaggcg ccgttgccgg atcggattcg tccccgcaat
     2281 ctggatgatt tcgtcggcca ggaacatctg ctgggttcgg gaaaagttct ccgccgcttg
     2341 atcgagcagg acaagctaag ttcgatgatt ttgtggggac ccccgggagt cggcaagact
     2401 acattggcta tggtgattgc cgccatgacc cacgccgaat tcgtcacatt ttccgcagta
     2461 acctccggga tcaaggagat ccgcgaggtg atgtctcagg cggaagccag tcgcagtctg
     2521 ggacggcgca ctatcctctt tatcgatgag attcatcgtt tcaataaagc ccagcaggat
     2581 gcctttttac cccatgtgga aaaggggaat atcattctca ttggggctac gaccgagaat
     2641 ccttcctttg aggtcaattc ggcgttactc tcgcgctctc gggtttttgt acttcatgcc
     2701 ttaaaaacag aagaaatcgt cgagttacta aaaaatgccc tccgggatga acggggtttt
     2761 ggaggaatgt ccatcgcgat cgatccggat cttttggaac ggatcgctgt ttttgctaat
     2821 ggtgatgcca gggctgccct aaatgttctg gagatggcgg taaatagcgc gccgcaggtc
     2881 cacgggagtg ttacaatcga tgcggccatc gttgccgatt ccttgcagca taaaaccttg
     2941 ctttatgata aaaaaggaga ggaacattat aacatcatct cagcgcttca taaatcgatg
     3001 cgcaatagtg attgcgacgc tgcagtctat tggctggcac ggatgttgga gggcggagag
     3061 gatccgttat acgtggcgcg ccgcttgatt cgtttcgcct ccgaggatgt gggtcttgcc
     3121 gatccgcggg cgctttccat cgccaacgcg gcatacgaag ccgctcatta cctgggaatg
     3181 ccggaatgca atgtgaatct aactgaagca gtggtttacc tggcattggc ccctaaatcc
     3241 aacgcccttt atcttgctta tgagagcgcc aaaatagacg ccgcacagac cttggcggaa
     3301 ggagttccgc tctggttaag gaatgccccg actaaactca tggcggaggt tgggtacggc
     3361 aagggttatc gctatgctca cgaatttgcc gccaaaacaa cgaatatgca gtgcctgccc
     3421 gataatctcc gggagaaacg atactaccat cccacggaac aaggactgga aaaaaacttc
     3481 gccgaacggt tggcgctcat tcgtaaactt aaggagaaag atccggccga aatttagggg
     3541 ttgggagccg gccgcgggta tggcaaattt tgactgtatc tgcaggaatt ccagagtaat
     3601 atgtagaata aatgcttaaa ttggatttct tccctatttt tatcatttca acatagattt
     3661 aaaaggaaga aacgataatg ccaaatctcc attcaaatag aaaaaacaca atgaagacca
     3721 cttaatggcg gtcttttttg ctatgtaccg ttttcgaact tgaacctcac cgtatggcag
     3781 agtatagatt taacggcaga gaaccaaggg ttctgctctc ccccgagtgt gggctcaccc
     3841 cattccaaag caaggcatgg atgcaactta gaacggttca caacgataga tgaggagtga
     3901 tgcctgtggc catccgcaag cgggtactat atgtagttgg tacggcttgc acgatattga
     3961 tcgtgcttct gtattccaca acgcagatct ttttggccag ccgtttcggg gccattgagc
     4021 gcgacagcgt ccatcaaagc ctgctccaag tggtgaatgc cttggataat gaacttcatg
     4081 atctgggtaa ggaaacggac gattgggcaa gctgggacga tacctatcgt tttatcgcga
     4141 cgggtgatcc caattatatt tcgatcaatc tacccgatga aacatttatg gatcttcaat
     4201 taaatctcat cctttatctg gacaaatcca accgggtcgt ttacgggaaa gctttcgatc
     4261 tgacaaaggc ccgtgagatc cgcgatccgc gaatatttac catccttgca ttgagcgaca
     4321 gattgacgaa tgtgaaagga gaacaaagtc gaaccgggat cgtcctcttg ccggaaggcc
     4381 ctttacttct ggcagcgcat gccattttga catccgaacg aaaaggaccc agccgtggca
     4441 gtttgatgat gggccggttt ttaaaccagg atgagattcg gaagttagcc acaattaccc
     4501 atctacccat tgccatctac ccatacgttc agtttcaaaa ccgttttcag gagaaacggt
     4561 ttcacctgga tctcgatcat cccaagttgg ttcagccatt aaacggtcat caggtcctag
     4621 gcggcaggct cttgagcgat ctctccggga atcccgtttt agtggctact gtccaactac
     4681 cccggaatat ttaccgtcaa ggccaggcca gtatctttta tttcggattg tccctggtgg
     4741 gtgtaggtgt ttttctagcg attttggtag ccggattctt ggggaaggtg gttttaaacc
     4801 ggctggcttc cctgaaccaa tgtatcaaac agattgcaat cagcagtgac ttgagcatcc
     4861 ggttgccagt cagtggtgcc gatgaactga gcaatttcgc cagagtctta aaccgtatga
     4921 tgaacaacct ggaacagatg catcagaaat tggaggatag taacaggcaa ttggctgcga
     4981 tcatcgagtt tctgcccgac gcaactttag tgattgatga agccggacgg gtcgtggcct
     5041 ggaaccgggc gatggaggag atgaccggtg ttccgaagca ggagattatc ggccggggtg
     5101 attatgccta tgcgattccg ttttacggga aaccccgtcc tctcttgatt gacctggttc
     5161 aatccgatcc cggggagatc aaacgttatt atgagcatac ggagaaaaag gggtcggtgc
     5221 tatattccga ggtttatgtc gataccatcg ggaaaaaaca agggggttat ttgtggatta
     5281 cggcttcacc gcttgtcgat caagaagggc ggctagtccg ggccatagag tccatgcgcg
     5341 atatcaccaa acggaaaaag atcgaggagg aactgaaata tctcagcctg cacgatccct
     5401 tgaccggcct ttatgaccgg gcttatttcg aagaagagat tcgccggctg gagcgcggcc
     5461 gggaaccggt tgtcggtgtg atcatgtgtg atgtcgacgg actgaaactg gtcaacgact
     5521 ctctcggtca cagtaccggg gataaactgc tgcaaacggc ggctaatgtt atccggtcgg
     5581 cttttcgcca gaacgatgtg gtggccagag tcggcggtga tgagtttgct gttcttttgc
     5641 cgcaatgcga tggaaccctt ttggaaacag caggccggcg cgtccaagag tctgttgaga
     5701 atcataaccg gagtaatccc gaattaccgc tcagtatctc gttggggtgg gctttccgga
     5761 aggaggcttc agtacgtctc aaggatgtat ttgaggaagc cgacaataac atgtaccggg
     5821 aaaaattgca gcaccatcaa agcaaccgta gtgcgattgt acagacactg atgaatacct
     5881 tacgggcccg ggactttgtt accgaggatc acgccgatcg tttaaaaaag ctggtggccg
     5941 atttatcgcg taaacttgga ctggatgaac aaaaaattca cgatatcagc cttttggcgc
     6001 agtttcacga catcggcaag gtggggatcc cggatcaaat cctttttaaa caaggaccgt
     6061 taactcagga agaatggaag attatgaggc gccataccga aatcggatac agcattgccc
     6121 attcagccca ggaattgatg catatttccg attggatcct gatgcatcac gaacggtggg
     6181 atggaggcgg ttatccgcgc ggcttgaaag gtgacgctat tcctcttgaa tgccggatac
     6241 tgagtattgc cgattcttac gatgccatga ccagcgaccg gccttaccgc aaagccctta
     6301 ctcattccca agccgtacag gaattgagaa aatgcgccgg tacgcagttt gaaccgaaat
     6361 tggtcgagga gtttattgag atgatcgggg agtagaccta tctcttgcga ctcacccctt
     6421 ttgtctcctc tctcccggca agcgggaaag aggggagaag gaaaatacaa ggcagtgaga
     6481 tagaccgcgg aggattgtgg ttacgcgcgc acatgtgcgc gactctagcg ctcgccgcta
     6541 gagggagcga gagagagtcg taggcaccta cgaaagaact taagtcacac gcttgcgtag
     6601 cgaaccggtc catgctatag ttatacctgt ttagttaatt ctaaccgttt cattttgagg
     6661 agcagccaaa tcatgagcga acatgagatc accgtctctc atatggtctt gcatatcctg
     6721 gatcccgaag ccggggttcc ggttttgtca aaccgggaga tcgatccgag tgaggatggt
     6781 tttgatttta cggagaagct aattcggaag atgctggacg atgagaattt gaagaacgcc
     6841 gtcttcttcg acgggccgaa ccggaccgcg gaactctgcg cttcattatt ctcgggtgca
     6901 acgggttttc tggacgcaac ccgggatctg gccgggggat tgttcgaaat catgagccag
     6961 caccctgaga ttccggcggc ggatctcatc tgctgcctgg cggaaatcga cggcgtccca
     7021 tatttgggat tgttaaagct aaattaccgg agcaacttta ctcatcaggt tcaatatgag
     7081 ggtaacgccc cggtgaattt gcttttcagg caacgggccg tactgccggg agagaatcag
     7141 aaaccggatg agggagtttt gattaattta caggaccgaa ccctgcggct ggttgaaaaa
     7201 caacatgaac ttgacgggga aaaggagtat tatctctcca aacgtttcct gggttgcggc
     7261 agtcaattgt ccagtgccga aaaggcaaag atcttaaacc gagttgcagt aaagctgggc
     7321 aagaagtata acaacgaaaa actggatagc tccgcccggt tgcgcaagtc cgttaccgaa
     7381 catctggaaa ccggcggtga agtggctttg actacggttg cccaggaaat ttttcgggat
     7441 gcccccaccg cgcaggagga atatgtctcc gaacttaaag aagccggaat ccgtgaagaa
     7501 aaggttcaat tttccgagaa gctggcggca cggaaattta aaaaccaaaa gatcaaaacc
     7561 gacaccgggg tagagatcga gttccccgcc acccattaca tgaataaaga tttgatcgaa
     7621 ttcgtccccc agcccaacgg aacactatcg attatcatta aaaatgtggg taaaattacc
     7681 gatcgatagt tggggaacag accgggaagg agcattgtca tgaacagcta tcatatgcgg
     7741 cgcaaggatc gtcaaatcac cgatcccgag atgatcgggg gagtattacg ctccgggaaa
     7801 tatgcggtga tcgcactttg ccgggagaat gaaccatacg tggttacctt gagttatggt
     7861 tacgacgagg ccggcaaggc gctttacttc cattgcgcac cgagtggact gaagctcgag
     7921 ttcatccagg ccaatccctg cgtttgtgcc acagtgatcc aggaccaggg gtatgtaccc
     7981 ggccagtgtg agcatcgtta tatttcattg gtcttgcggg gacgaatgac gatcatcaac
     8041 gacctgcccg gcaagaaaca tggcctggac atactcctgg cccatctgga ggacgatccg
     8101 gctccgattt tagcccgtaa tatcaaggat caggcttcat acgaccgctt tgtaatgcta
     8161 cggatggata tcaccgagtg cagcgctaag cagggtagct aggtcggagc aggaaggagt
     8221 atatatgaat ttgttagcgg ctgaggatat cagcaaaagt tacgggacga aagctttatt
     8281 ttccgggtta aacctgggag taaacagcgg cgataaggtt ggattgatcg gagttaacgg
     8341 gaccggtaaa tcgaccctcc tcaagatcct gatgggcata gaaccgccgg atagcgggcg
     8401 gattattcat gcaaccaatg cccggatcgg ttatttgcaa caaaatccgg tatttaaaga
     8461 gcgaagcacg gtgatccaag ccgcgctcca agggaactca ccccaaatgc aactggtgcg
     8521 cgagtatgag tcggccttgg cagaggcgga agctgccccg gatgatcata agattcgggc
     8581 gcgcttagct acactcggcg agcggatgga tgaagccggc ctttggcaga tggagagcca
     8641 ggcgaaaatg gtcctgacca agctcggtat ctctgatttc tcagccgtgg ttgacacact
     8701 ttccgggggt gaacggaaac gaatcgcctt ggccgcggcg ctcatcaatc cggtggatct
     8761 gttgatactt gacgagccga ctaatcagat cgacgccgac actgtcgctt ggttggagac
     8821 atttttaaaa gagcgccagg aagcgctgtt gatggtcact cacgaccggt attttttgga
     8881 ccgggtaacg aaccgcatcg tcgaattgga ccggggtcaa ctttacagtt actccggaaa
     8941 ctatagtttg ttcttagaga aaaaggccga acgggaggaa caggaacagg ccacggcggc
     9001 caaaaggcag aatttgttcc gcagggaact ggcgtggatc cgcaggggcg ccaaggcgcg
     9061 ttccacgaag caacaggccc ggatcgatcg ttttcaaaaa ctcgaaactg agatcactac
     9121 gaccggtccg gcccgggaga tggagatcac cgcgggcgcc agccgtctgg gaaaaaaggt
     9181 agtcatcttg gagcaactgg gtaagagttg ggacgggcgt ccgatcatca ccgatctgga
     9241 ctataccttt ttgcccggtg atcgagtcgg catcatcggt cccaatgggg taggcaaatc
     9301 gactttactg aggctgattg cagggatgat tcaaccggat acggggcggg tggagatcgg
     9361 ttctacagta aaatttggtt ttttcacaca ggaagctctc aaaggggatg aatcgcaaag
     9421 ggtcatcgat tacctcaagg aacaggccga gtatttggct atcgctgacg gacaacgaat
     9481 cagcgcttcc caaatgttgg aacggttcct gttttcgccg gatatgcaat ggacccccat
     9541 cgccaaactc tccggcggtg aaaagcggcg tttgtttttg ttacgggtcc tgatgaccgc
     9601 ccccaatgtg ttacttctgg acgaacccac caatgacctt gatactcaaa cattgggcat
     9661 cctggaggaa tacctcgatg ggtaccccgg tgtagtgatt acggtctccc acgaccgtta
     9721 cttcctcgac cgggtggtgg agaagttgat tgccctcgag cccgatgggt ccatccgcca
     9781 ttttaccggg aattacagcg attttttgga ggaaaggttc gaaaagtccg gactttcttc
     9841 atccggaaag aaccgggaac tgccggcccc agagccgaaa ctgccggaga aaacggcaca
     9901 tcccaaaccg cagctcaaac tgagttataa ggagcaacgg gagctggatg gaattgaagc
     9961 ggtcattgac gcaaccgagg ggaggctggc tcgggtcagg gagcggatcg cccaggcagg
    10021 cagtgattat caagccttgc aggaattgat ccgtgaggaa caagacacgg atcacgagtt
    10081 acaggagttg atgaatcgtt gggtttatct taatgagttg gccgcggagg ttgccaggaa
    10141 taaatcgtca aaaacaaaca gcggcagtta gaaaatgttt taattctata aattttcctg
    10201 tataatattt ttggtggctt tggagctgct aaattattct gagaggagac caacggttca
    10261 tgaatacggt tcaggtgagt ttcgattatc cggaacggtt atcccggggt attctgttgt
    10321 tgaagacctt tttaggcttt atcatcctca taccccattt gatcgccctc ttcgcattgg
    10381 ggattgccgc cggactggtc atgttcatca gttggtttgc ggtcttgttt accggaaagt
    10441 atccccagtc cctctttgat tttgaagtcg gcgtgcagga ttggggtctg cgggtctccg
    10501 cgtacatggg gctgcttcgg gatgagtacc ctgttttcgg tctagaggct cgatatccct
    10561 cacgttttca ggtccattac cccgataaac tctcccgcgg cattttactg ctgcgtcttt
    10621 ttttcggatg gatttatgtg atgatccccc acggagtctg tctgcttttc cggggaattg
    10681 cctatctcgc cgttaccttt gtagcttggt gggcggtcct tttcaccggg aagatgcccc
    10741 ggaggatgtt taatttcctt gccgggacct tgcggtggaa tcagaacctc aacgcgtatc
    10801 tgatgttttt aacggacgag tatccgccgt ttaccggaaa agaaaacgga tccggttcag
    10861 ccggaactgg gttcatcgcg taatcggtcg gcatgatttt gtgtggttta ttacagccgt
    10921 tgcctttatg gcaacggttt ttttacacct aaccggtttt cggatttttg ttacattcgg
    10981 atcataatta agggatattt tcgtagtaat cttgaatcgg atttgccatt agaagacagg
    11041 ccgcttcgat cggtgtaccg atgtgaccgg tgttatttgg gagaaactta atcgataaac
    11101 gaggcgaggt actgtaaatg ttcaaattcg caacagttca aatcagactt ggtaagagtt
    11161 ttagagcttt aggacaccgt gatttccgtt atttctggac cgggcagtgt atctccctgc
    11221 tcggtacatg gatgcagcgc acggcgcaac agtggctggt ttatagcctt accaaatccc
    11281 cttttttgct gggaattctg ggggtattcc aatttgggcc gatgttgctt ttttcattat
    11341 tcgccggaac tttggtggac cgctttccga agagacgggt gctgatcttt acccaaacgg
    11401 tattaatgat tcaggcgctc atcctggcac tattggtctg gaccggtcag attcagtatt
    11461 ggcagatttt gattctggcg ctcgtgatgg gatttgtaaa taccctggat atgccgaccc
    11521 ggcaatctta cttcatcgaa ctggtgggca aggatgatct gatgaatgct atcgctctaa
    11581 actctgccat cgtcaatatc gcaaaaatcg ttggcccggc tatcgcgggc atcgtcatgc
    11641 tctacctggg tacagcgatt tgctttttcc taaacggcat tagtttcatt gcggttttaa
    11701 tcggcctttt cctcatccgg acttcagata cccggttacg cgcacaaaac accaaactgc
    11761 tggcggatgt cggagaagga ttgaaatata tcgcctccaa accggtgctt tggagtactg
    11821 tcctggcaat gttcgcggtc ggaacctttg ccatgaatat ggatgtattg attccggtct
    11881 ttgccgggga ggttcttcac aggcaggcca gcggttatgg ctttttgttg gcggcgatgg
    11941 gaatcggatc gctggtgggg gcattgttgg tctcgacccg ttcccggcgg ggattaagcg
    12001 ctaaaattct gttcggcagt gccgtgatag aatgtttgtt ttatctggtt ttaggatttg
    12061 ttcatcatta cgaattggcg atgttcttca tcgccgtaat cggctttttc agcatcatct
    12121 tcatgaccac tgccaattcc acagtgcaat tgaattccga cgacgggcat cgcggccggg
    12181 tgatgagcgt atacagcctg gcattcgcgg ggactacacc catcggtaat ttttttgcgg
    12241 gaagcgttgc cgaaaaaatg ggaggaaact tcagcttcgt cgcatgcggc gcggtgacat
    12301 tggcctgtat cgtttggatt ttgctcaaaa caaagcgaca tgtaaacttg gtttccgggg
    12361 attttcacaa tccggacaat ttggtgtaga aatcgatcaa atacaatttt gccttgtttt
    12421 attcccgggc atgatccttc accatcagta ccacggtggt cacgatggcg atgaagcaga
    12481 ccacctggac cggattgacc ggaaggctga agatgggata agcggcgagg atggggtaga
    12541 agacggtggt ggcaaattgg gccgggccca ccaggatcat cgaccccatc cggtacgcca
    12601 tttgaatggc caccatcgac agggcggaga cggtaaagaa acaataaaga tagggtgaac
    12661 cgaggtagga aagcagttgg gtgccccgct ccagttgaag aatcttcatg taggtgatca
    12721 tcagcccgct catactaccg ctggccgccg cgagccaaag tgcctgcaag ccggaggtcg
    12781 gccgtaatac cttggcaact cgcaaaagaa accaaataaa gagcgaacct acgggtatcg
    12841 ctgcggccaa ataagcgaaa tcagcctgaa tagtcgatac ggatgcaccg gaaccatcgg
    12901 ccagattgat gcccacaatg gcggcacaca taatgagcgt atacaaatag tcctgcggat
    12961 ggaggggttc attgagtatc catcgggaga ggaagatcat gaacacgatg ttcaagccgg
    13021 agatggcatt gacgatatac gcgtttaaga ctccaagtga taaataattg ggtaataccg
    13081 ccagattgag taaggcaaaa ccgattcccc aagtccagag gtcccggtag tatccgggtc
    13141 ctttccgccc ctgaaacccc aaccagccga tacccttctt ttgcagcaca aaccccaaag
    13201 aggtgcaaaa gtatcccaag acagcctcac ccagggcgat cacagtgcga aatagcattg
    13261 attatcacat ccgttaacaa gttgtatgaa cacctgtgtg accaacaatc taacaaatca
    13321 gagcttaatc gaggcgatgg tgtagcaatt tacagaggaa tcaggcctgt ccgcctcttg
    13381 cacagtgata gtgtctttaa cttctttcaa aagaagggct
//
LOCUS       DASVMB010000247         6558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_546042,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000247 DASVMB010000000
VERSION     DASVMB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546042"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VF531_07275"
     CDS             <1..1484
                     /locus_tag="VF531_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7713809.1"
                     /translation="GLEMVLVLYDPGAGEVIYRDAFQAPEEQFLAEWEERLQNLLRSL
                     HGGVEESATTPRMYTRSLDAFLAFRRGLEILSQAKNESSRGEGLESLLQAVAYDPGFL
                     EAIDILLLFLMQSDIVHNYDYSISILERLHLIAGNHPRIPLVMAEVYYQLGNKDKTEQ
                     LLKNLVQTLPQFTEGWIRLAFFYHTNDQPDAALETLQSVLAYEPHEATVLDMMGAIHA
                     GRGENELARKLWLEALETEPSRVNILNNLALLSEEQEDLVQAELYYQQALKVNNEWWG
                     SYFHYGTFCFRHSRYEEAAGWLAKAAELNPVHPQTFQNYGLTLIKLGRFHDAQENLLR
                     LLQLAPDNLTRRQTLQLLNQLNDPAAKTEVRIRQLEKLWDNGERSRAILGMAGQIWKA
                     RHSWYYWYLWGRIFEAAGIKLPMRLVWSIAMNYGPGFPLLKKVGLYYWEKKRYRTALP
                     MLRKAFELHQSDPDTVHAYFQTLFYLGEVEELQANMNGLHQQI"
     gene            1494..2306
                     /locus_tag="VF531_07280"
     CDS             1494..2306
                     /locus_tag="VF531_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7713810.1"
                     /translation="MSRTRKEGEGETVQAVIRALSLLEILARENQAISISEIAKKSGL
                     KLTTAHRLLNTMMNRGFVEQDENTLQYRLGIKAFEVGNAALVASDLRTIARPYLKRLM
                     EQVNETTNLAVLDGTEVVYVDQLESTNIVIVKMFARVGSRGPAYCTGTGKVLLAGLAD
                     ADLRQGFARVNFTRFTPRTITNIEDLIGALVQIRRDGYALDFSERDEGVTCVAAPIKN
                     FENRVYAAISVSGPASRMTEPRIKAEILPAVLHAAAGISERLGYRNIGAERA"
     gene            2474..2977
                     /gene="rimP"
                     /locus_tag="VF531_07285"
     CDS             2474..2977
                     /gene="rimP"
                     /locus_tag="VF531_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7713811.1"
                     /translation="MPKERIEDLVSRLAEPLAQELGYELVEVEYHKEGPNWVLRCMID
                     YPAGVGLNECQRFSEALSELLDREDPIPGSYLLEVTSPGVERPLVKDRDFIRFTGNRI
                     EVRLNQPIGGSRRLQGLLTGVRDSENEKWIQIDINGSMMEIPRKTIVKAHLISEIFGR
                     EGGRKKQ"
     gene            2974..4632
                     /gene="nusA"
                     /locus_tag="VF531_07290"
     CDS             2974..4632
                     /gene="nusA"
                     /locus_tag="VF531_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7713812.1"
                     /translation="MNHEFLDALEQVEREKGVSKEILLDAIETALASAYKRDQKVAQS
                     IEVRIDPDTGEFHVFTHKTVVEEVEDDKNEINLADAQRINPVFELGDLVEFEIFPKEF
                     GRIAAQTAKQVVVQRIREAERSIIYDEFVNRVGDLITGIVQRFEQRNIFIDLGRIEGI
                     LPGNEQMPNEHYEPGTRLKTYVVEVKKTTKGPQVLLSRTRTGLLKRLFELEVPEIQDG
                     LVEIKSVAREPGFRSKVAVVSRDAQIDPVGACVGARGIRVQMIVRELKGEKIDVIPWS
                     SEPIEFIVNALSPAKVSEVKILPSKKIAIVIVPDFQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSETQAAEQAEEIERMIREEIGFRELSETPEPVETAEGNGTTEAEEVLATENLPG
                     EPQEEPMSPDEIEAESEEPFEPEPVETEPGTVPAVEEEEEEYAPKKKTKKSKNVPKHL
                     ERLELEEEAFPSPEDHYFFSDVLSKNQESASPEALETSETKSFKSEGEKQDESGFTGF
                     SIGQLLQKELAKTGTAKKEPAKDGNKPRSAKERKKGGTVKKDED"
     gene            4622..4894
                     /locus_tag="VF531_07295"
     CDS             4622..4894
                     /locus_tag="VF531_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX7713813.1"
                     /translation="MKIKKIPQRTCLGCKTIRPKKELVRIVRTPEGEVLVDPTGKKSG
                     RGTYICPNSNCLEAAFAGSLLDKALEIKVTPEIRERLKNELSSLTR"
     gene            4891..5193
                     /locus_tag="VF531_07300"
     CDS             4891..5193
                     /locus_tag="VF531_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEX7713814.1"
                     /translation="MNSITTLLGMAARAGRLVSGTAAVEAGLKRQQVLLVICATDLAP
                     RTIRNFSFWCEKYHAGFFCYGTRSELGKCIGRPERGLIGVTGVDFAGAIRSRLEEK"
     gene            5239..>6558
                     /locus_tag="VF531_07305"
     CDS             5239..>6558
                     /locus_tag="VF531_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7713815.1"
                     /translation="MSAAAKTVIIFRLGWFIINKIRVHELSKELGIQSKELMNFLNKL
                     GANVRNHMSTIEDNFVEQARQAFPAPQKQSRFPQLKPATPEGVKPQQNAAVSKEPVTQ
                     PYSAKVAPGNAPKPRAATPVSGPNRPPVTNQAAANPETRRSMPGASGVNQAPVKPAPV
                     NNQIPKQAPANQAPKQAAPAFQPGGKPSGPMAGKPQPQVNPHARTESRGPVRPGTTGN
                     QGGFSGRPQAQAQAQGRVDSHPQNRPHPPERNDAPRDNRGAGANKFSGQHGVERHPSG
                     QPSRNPRPNQNQPNRGGKPATTRPGHDFHAGNAHGQTGTGPNRGRWNNQQQHNKSFAP
                     AKPVKPQGPATTVKSVQLPDVMSVKDFAHLIEIAAGDVIKKLIGLGVMATINQDIDRD
                     TMTLIAGEYGITASAAPTEEEKLRVEEIEDDPKSLVSRSPVVTIMGHV"
BASE COUNT         1716 a         1559 c         1832 g         1451 t
ORIGIN      
        1 agggcttaga gatggttttg gtgttatatg accccggcgc cggtgaggtc atttatcgcg
       61 atgcgtttca ggctccggag gagcaattct tggcggaatg ggaggaacgc ctgcagaatc
      121 tgcttcgtag tcttcatggc ggcgtcgaag aatccgccac gactcccagg atgtataccc
      181 gatcgctgga tgcttttttg gcgtttcgtc gcggacttga gatattatcg caagctaaga
      241 acgaatcctc ccggggtgag gggctggaga gtctcttgca ggctgtcgct tatgaccccg
      301 gttttcttga ggctatcgat attctattgt tatttttgat gcaaagcgat attgtacata
      361 attacgatta ttccataagc attttggagc ggcttcatct gatcgccgga aatcatcccc
      421 ggataccgct agtcatggcg gaggtttatt atcaacttgg caataaggat aaaaccgaac
      481 aacttctaaa gaatctggtt caaaccctgc cgcagttcac cgagggttgg atacggctgg
      541 ccttttttta ccataccaac gatcaacccg atgcggcgct tgaaaccttg cagtcggtat
      601 tggcttatga accccacgaa gcgacggtcc tggatatgat gggtgccatc cacgccggca
      661 ggggagagaa cgagcttgcc cgtaaactgt ggctggaagc tttggaaacc gaaccatcgc
      721 gggtcaatat cttaaataac ctggctttgc tctccgaaga acaagaggac ttggtccaag
      781 ccgagcttta ttatcagcaa gctctaaagg tcaacaatga atggtgggga agttacttcc
      841 attacggcac tttttgtttc aggcatagcc ggtacgagga agccgcaggt tggcttgcaa
      901 aagcggctga gctaaacccg gtacatcccc aaacgtttca aaattatggt ttaaccttga
      961 ttaaacttgg ccgttttcat gatgcccaag aaaatttgct tcgcctcctg caattggcgc
     1021 cggataatct tacccgacgt caaactttac aattattgaa tcaattgaac gacccagctg
     1081 ctaaaaccga agtacgcatc cgccagctgg aaaagttatg ggacaatggt gagcgcagcc
     1141 gggccatact cggtatggcc ggtcagatat ggaaagccag gcattcatgg tactattggt
     1201 atctttgggg ccggatattt gaagctgccg gaattaaact tccaatgcgg ctcgtttggt
     1261 ccatcgcaat gaattacggt ccgggatttc cgctattaaa gaaagtcgga ttgtattatt
     1321 gggaaaaaaa gcgataccgc acggctttac ccatgcttcg caaggctttt gagctccatc
     1381 agagcgatcc cgataccgtt cacgcctatt tccagaccct tttttatctg ggagaggtgg
     1441 aggagttgca ggccaacatg aatgggctgc atcaacaaat ttgaggtgat atcttgagtc
     1501 gcacgcgaaa ggaaggcgag ggggaaaccg tccaggctgt aatacgggcg ttatcactcc
     1561 tagagatcct ggcccgggag aaccaggcga tttcaatttc agagatcgcc aaaaagtcag
     1621 ggttgaaact taccaccgct catcgtttgc ttaataccat gatgaatcgg gggtttgtcg
     1681 aacaggacga gaacacgctc cagtaccggc tggggatcaa agctttcgag gtcggaaacg
     1741 cagctctggt cgcaagtgat ctgcgcacca tcgcccggcc ttatttgaag cgtctcatgg
     1801 aacaagtcaa tgagaccacc aatctggcgg tactcgacgg gactgaagta gtctatgtcg
     1861 atcaattgga atcgaccaat atcgtgattg tcaaaatgtt cgcccgtgtc ggcagcaggg
     1921 gaccggctta ttgcaccggc accggtaagg tgctactagc gggtctcgcg gatgcagatt
     1981 taaggcaagg cttcgcccgg gtaaatttca ccaggttcac cccacggacg attaccaata
     2041 ttgaagactt aattggagca ttggttcaaa tcaggcggga tggctatgcc ctcgatttct
     2101 ccgaacgcga tgagggggtt acctgcgtag cggcaccgat taagaatttc gaaaacaggg
     2161 tttatgcggc cattagtgtt tccggaccgg cgtcgcgaat gactgaaccg cggattaaag
     2221 ctgaaattct gcctgcggtg ttacatgcgg cggccggcat atcggaacgg ctcggttacc
     2281 gtaatatcgg ggcggagcgg gcttaatggg accgaactgg taagcggcgt aaaaaattgg
     2341 cgcttgcagc ttctgctagt gcagaaagca cttgccaaaa gggttcgggt atggtaaact
     2401 aatttggaga aatttctgaa aggagtgggg cgatcccact ctttccgttt tacacggaag
     2461 aggttatgaa gcggtgccga aagaacgtat tgaagatctg gtatcgaggc tcgctgagcc
     2521 gctcgcccag gagctggggt atgaattggt tgaggttgag tatcataagg aagggcccaa
     2581 ctgggtatta cgctgcatga ttgactaccc ggccggagtc ggcctcaatg aatgccaacg
     2641 ttttagcgaa gccttaagcg aattacttga tcgggaagat cccattcccg gcagttatct
     2701 tttagaagtg acatcgcccg gggtggaacg gccgcttgtc aaggaccggg acttcatccg
     2761 attcaccgga aaccggatcg aggtgcgatt gaatcaaccg atcgggggtt cccggcgctt
     2821 acagggttta cttacgggag tccgggattc agaaaatgaa aaatggattc aaatcgatat
     2881 aaacggttca atgatggaga ttccccgaaa gacgatcgtt aaagctcatt tgatttcgga
     2941 aatcttcggc cgagaagggg gaaggaaaaa gcaatgaatc acgagttttt ggatgccctg
     3001 gagcaggtgg agcgggagaa aggcgtaagt aaagaaattt tattggatgc gattgaaacc
     3061 gctctggcct cggcctataa gcgggatcaa aaggtcgccc agagcatcga ggtacgtatc
     3121 gatccggata cgggcgagtt ccatgttttt acccacaaga cggtcgtcga ggaagtggaa
     3181 gacgataaaa acgagatcaa tctggcggat gcccagagaa ttaaccctgt gttcgaactg
     3241 ggtgatctgg tcgagtttga gatctttccg aaagaattcg gccggatagc cgcccaaacc
     3301 gccaaacagg ttgtggtgca gcgaattcgt gaggcagaac gaagtatcat ctacgatgag
     3361 ttcgtcaatc gggtaggaga tctaatcacc ggtatcgttc aacgctttga acagcggaat
     3421 atctttattg atctgggacg gatcgagggg attctgcctg ggaatgaaca gatgcccaac
     3481 gaacactacg aaccgggcac acggcttaaa acctatgtgg ttgaggttaa aaagactacc
     3541 aaaggccctc aagtgctgct ttcccgtact cggaccggtt tactgaaacg ccttttcgag
     3601 ctagaggtac cggagataca ggatggattg gtggagatca agtcggttgc ccgggaaccg
     3661 ggtttccgtt caaaggtcgc cgttgtcagc agggatgcgc agattgaccc ggtcggggct
     3721 tgtgtaggtg cccgcggaat tcgggtacag atgattgttc gcgaattaaa aggggagaag
     3781 attgacgtta ttccctggag ttccgaaccg attgaattca ttgtgaacgc cttaagcccg
     3841 gccaaggtca gcgaggttaa aatcctgccc tcgaagaaaa tagcgattgt gattgtaccc
     3901 gattttcaac tttcactagc catcggcaaa gaaggacaaa atgcgcggct tgccgccaaa
     3961 ctaaccggat ggaagattga tatcaagagt gaaactcaag ccgcagaaca ggctgaagag
     4021 attgaacgta tgatacgcga ggagatcgga ttcagggagt tgagtgaaac tccggaaccg
     4081 gttgaaacag ccgaagggaa cgggacaacg gaagccgagg aagtattggc gacggaaaat
     4141 ctccctggcg agcctcagga ggagccgatg agccccgatg aaatagaggc tgaatccgag
     4201 gaaccttttg aaccggaacc ggttgaaacg gaacccggga cagtacctgc cgttgaggaa
     4261 gaggaggaag agtacgctcc gaaaaagaag accaagaaat ccaagaatgt tccgaaacat
     4321 ctggaacgtt tggaacttga ggaagaagcc ttcccctccc cggaagatca ttatttcttc
     4381 agcgatgttt tgagcaagaa tcaggagtcg gcttccccgg aagcgcttga aacatccgaa
     4441 accaaatcat ttaagtccga gggtgaaaaa caggatgaat ccggtttcac cggtttttcc
     4501 atcggccaat tgcttcagaa ggaattagcc aaaaccggga cggccaagaa ggaaccggcc
     4561 aaggacggga ataaacctcg ctccgctaag gaacgcaaaa aagggggaac cgtcaagaag
     4621 gatgaagatt aaaaagattc cgcaacggac ttgcctgggg tgcaaaacga ttcgccccaa
     4681 aaaagagttg gtccgcatcg ttcgaacgcc cgagggagag gttttggttg atccaaccgg
     4741 taagaagtcc ggccggggaa cctatatttg cccaaattct aattgcctgg aagcggcgtt
     4801 cgcgggatcg ttgcttgata aagcccttga aataaaggtt actcccgaaa tccgggaacg
     4861 tttgaagaat gaactgtctt cattgacgcg atgaattcga taacaacatt attgggcatg
     4921 gctgccaggg ccggccggct cgtaagcggc acggctgcgg tagaggcggg tttaaaaaga
     4981 cagcaagtgt tactggtcat ctgcgccacg gatcttgcgc cgcgaaccat tcgtaacttc
     5041 agtttttggt gtgagaaata ccatgccggt ttcttttgct atggtacccg ttccgaattg
     5101 ggaaaatgta tcggacgtcc ggaacggggc ctcatcggtg tgactggagt tgattttgcg
     5161 ggcgcgatcc gttcccgcct ggaagaaaaa taagcggttg gattatgcgt gcgtgagcga
     5221 agcaagtccg gaggagcatt gtctgcagcc gcgaaaaccg tgattatctt tagattgggg
     5281 tggtttatta taaataagat ccgtgtacat gaactcagta aggaattggg gattcaaagc
     5341 aaggaactta tgaattttct gaataaattg ggtgccaatg ttcgtaatca tatgagcacc
     5401 attgaggata actttgtgga gcaagcccgt caggcatttc cagccccgca aaaacaatcc
     5461 cgctttccgc agttaaaacc cgcgactccc gaaggtgtga aaccccaaca gaatgccgcg
     5521 gtatccaagg aaccggttac tcaaccgtat tccgctaaag tggcgccggg taacgctccg
     5581 aagccgcgag cggcgacccc cgtttccggc ccgaatcgtc cgccggttac caaccaggcc
     5641 gcggcaaatc cggaaacacg ccgtagtatg ccgggcgctt ccggggtgaa tcaggctccg
     5701 gtcaaaccgg ctcctgtcaa caatcagatt cccaaacaag ctcctgcaaa tcaggcccct
     5761 aaacaagctg ctccggcctt tcaacccgga ggtaaaccgt ccggcccgat ggcaggcaaa
     5821 cctcaacccc aggttaatcc gcacgcacgg accgaatccc ggggaccagt gcgcccgggt
     5881 actacaggga atcagggtgg tttttcagga cgtcctcaag ctcaagctca ggctcaaggc
     5941 agggtggact ctcatcccca aaaccgtccg catcccccgg agcgaaacga tgcgccgcgg
     6001 gataatcgtg gagcgggagc caataaattc tcaggccagc acggagtcga gcggcaccct
     6061 tcgggccagc cgtctcgaaa cccgcgcccc aatcagaatc aaccgaaccg gggcggcaaa
     6121 cctgcgacta cccgtccggg gcatgatttt catgccggaa acgcacatgg tcagaccggg
     6181 acaggtccga accggggccg ctggaacaat cagcagcaac acaacaaatc ttttgctccg
     6241 gccaagccgg tcaaaccgca aggtccggca actaccgtta aatcggttca gttgcccgat
     6301 gtcatgtcgg tgaaggattt tgctcatttg atcgagattg ccgccggaga tgtcattaag
     6361 aaactgattg gtttaggggt catggccaca atcaaccagg atattgatag agataccatg
     6421 acgttgatcg ccggtgagta tgggattacc gcatcagcgg cgcccaccga ggaagaaaaa
     6481 cttcgggtgg aggagattga ggacgatccc aagtcgctgg tctcccgttc accggttgtc
     6541 accatcatgg gccatgtt
//
LOCUS       DASVMB010000248         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_619839,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000248 DASVMB010000000
VERSION     DASVMB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619839"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF531_07310"
     CDS             complement(<1..485)
                     /locus_tag="VF531_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX7713816.1"
                     /translation="MTTYFHKCTDPLIRITDDARAYTRPQAKSVWGGGAIFLDRDGTI
                     IVEKNYLREIEDLKLLPGAVPGLKKLGATGMPLYLLTNQAGIAHGYFDENRVDAIHRY
                     LIDLLATAGIHFRGVLYCPHHPQAEVAAYRTDCFCRKPKPGLLLHAAYLDSIDLSRSF
                     V"
     gene            complement(615..1625)
                     /locus_tag="VF531_07315"
     CDS             complement(615..1625)
                     /locus_tag="VF531_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX7713817.1"
                     /translation="MEIRSRAPLRVSFAGGGTDIEPYLSEKGGVVLSTTINRYSYGTL
                     IPRNDQEIQIESLDVEVFAKFMASQKLDFNGQLDLVKAVLRKFDGWKQGFSLFLHSDA
                     PPGSGLGSSSTMIVTLLGLFRQWLSLPWNNYDLASLAYEIERCDLGIRGGKQDQYAAV
                     FGGFNFIEFYHDTTVVNPLRITAAILNELEYHLLLCYTGKTRLSANIIATQVDGYLKK
                     SESSLHALDQLKAITVAMKNALLQGHLNQFGDLLHQGWMEKKQLAGAISNEKIDSLYH
                     LARNKGALGGKILGAGGGGYLLLYCPYNRKHIIAAELEKVGGQITPFSFENQGLTTWR
                     VE"
     gene            1906..3444
                     /locus_tag="VF531_07320"
     CDS             1906..3444
                     /locus_tag="VF531_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713818.1"
                     /translation="MLKSREVEQGVKGDHPPDSQAAAGFLGRKTEYGRWAPLLERSGL
                     KPRRIMPVTDAIYVDTSEGWFRIGRRSYPRAELTWLRSILEYLEQRSFRGWAVPWQKT
                     IIWEEGGHCYLVQPWLDDSGGFQPDDPASLARFVEVLTEFYRCGKEYTRNGHEICRDR
                     FSSIPREWEMARKQLAEFESGRFSEKHRNEWRDMLKKAQDRIALNLKKWEDSGAGGII
                     GQQSTTGVIGHGKLLARYIVGRDHDFALLNWEHLAFQPRIQDLATLILDLKLWEPEWL
                     LFLISEAAKAQPFWPEEYSALFTILWHPQELLDALKNSEEDQDRKNLKEAGKEMARKE
                     RCLTKAWRELGTEKRWAFRNPLAENLLPDGKVSWVLSPVESWGDTGGAMDSLIRVQNP
                     AYLPPEVMQRLSYGLPDRIVGGGDADVPKAANAEMDRVAGYSVSDFQTSQTVPAEAAN
                     LSSPEKSEPVIPVQHQEAAETPVEPPQPNDMPRHTVTAPSSTQTLRWANFPKKQGAAK
                     LDQK"
     gene            3431..4477
                     /locus_tag="VF531_07325"
     CDS             3431..4477
                     /locus_tag="VF531_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713819.1"
                     /translation="MIKNEFELYQKVAGIDPENRMMGLAPAPPIRMEQFAPDGWRWEI
                     TGGVMALWTFHGSEGALAYQHQMLKAFESQNTRGFLYPLPLSDGRTYAQLGERQWFYF
                     TSLPPPGRVSFGNLKDLESVVELLVDFRKACERGGIWCCLPERKTTSNLLHRFQAILE
                     HLKVFAALGKHRLRPTWFDRIFLQRLPEAESQAIEAIRYLETTRYFELWERLTGQDLI
                     LNKLVRSNLRLTPKREAVCLRVHDFSWDPPIIDLANLLVKTGRSAHWTPKWFSGVTGK
                     YRESFSISDTEWEVIKAFLLFPWSFYRLASRYYYNRTEWSHETFITKLERLWDDEPNR
                     RQFLDELLKKGGLI"
     gene            4754..5230
                     /locus_tag="VF531_07330"
     CDS             4754..5230
                     /locus_tag="VF531_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7713820.1"
                     /translation="MFNIGDKVVYPMHGAGVIESIEEKEISGEKLRYYIIKLPMGEMK
                     AMVPMDKVKEVGLRQVIDEEEILKVFEVLKSESSSMPLNWNHRYRANLEKIKSGSIYE
                     VAEVVRNLASRDRQKGLSTGEKKMLENARRILLSELVLAKNVTETTMVEILEGYLN"
     gene            5545..>6141
                     /locus_tag="VF531_07335"
     CDS             5545..>6141
                     /locus_tag="VF531_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX7713821.1"
                     /translation="MSKPSIIGWGFIVGGLLGVVFVLSVLPAAGWQTWLIAGLVGAVI
                     IGAITVAAQNLIRGIIKKTNGEELLIKTGSFIWGLFTTALLLGIFRPIMNGMSQTLNW
                     TLTILLFLVCTGFCVGVGSLKAKEICMLIFTATAAPGQKAVPGKNQLKILDTSAIIDG
                     RIADLCNTGFLEGVLIIPNFVLNELQKIADSADPLRRNR"
BASE COUNT         1550 a         1384 c         1630 g         1577 t
ORIGIN      
        1 atcacaaaac ttcgtgaaag atcgatggag tcgagatatg ccgcatggag cagtaatcca
       61 ggtttgggtt tgcggcaaaa acaatcggtc cggtacgcgg caacttcggc ctgagggtgg
      121 tgggggcaat agagcacgcc ccggaaatga atccccgccg ttgccaacaa gtcgatcaaa
      181 tagcggtgga tcgcatcaac ccggttttca tcaaagtaac cgtgggcgat acccgcctga
      241 ttggtcaata agtaaagcgg cataccggtg gcaccgagtt tttttaggcc tggaacggct
      301 ccggggagta acttgagatc ctcaatctcc cgtaagtaat tcttttctac gataatggtg
      361 ccgtcccgat ccaaaaagat cgccccgcca ccccagaccg acttggcttg tgggcgcgta
      421 tatgcgcgtg cgtcatctgt aatccggatt aagggatcgg tacatttatg aaaataggtg
      481 gtcatattgg ggctcctcgg gtcgatgcct gatggcagta gttggcgaca acaagttttt
      541 aggcattgtt atagctattc tacctttcag gtggttattc catcctccag atagttgttc
      601 catcctccgg atggttattc caccctccag gttgtaaggc cctggttctc aaaactaaac
      661 ggagtaatct gtccccctac cttttcaagt tcggcggcta tgatatgttt tcggttgtat
      721 gggcagtaca aaaggaggta accgccgccg ccggccccca ggatctttcc gcccaaagcc
      781 cctttatttc gggctagatg gtacagacta tcgatcttct cattactgat cgctccggct
      841 agttgtttct tttccatcca tccctgatgc aacaagtctc cgaattgatt caaatgcccc
      901 tgaagcagag cgttcttcat tgcaaccgta atggccttca attgatccag ggcatgcaac
      961 gaactctcgc tctttttcag gtatccatcg acctgagtgg cgatgatgtt tgccgacaac
     1021 ctggttttgc cggtataaca gagcaaaagg tgatattcga gttcattcag gattgccgca
     1081 gtaatccgca gcggattgac gacagtggta tcgtggtaga actcaatgaa attgaatccc
     1141 ccaaaaactg cggcatattg atcctgttta cccccgcgaa tccccaagtc gcaacgctca
     1201 atctcatagg ccagggacgc cagatcatag ttattccatg gtaagcttag ccattgccgg
     1261 aacaagccca aaagggtgac tatcatcgta gaggaggaac ccagcccgct accgggtggg
     1321 gcatcgctat gcaaaaacaa ggagaagccc tgtttccaac catcgaattt acggagtacc
     1381 gccttgacca gatccagttg gccgttgaaa tccagttttt gtgaggccat aaatttggca
     1441 aagacctcta catccaggga ctcaatctgt atttcctgat cgtttctggg aatcaatgtt
     1501 ccgtaactat agcggttaat tgtggtgcta agcacaaccc ctcctttttc tgaaagatag
     1561 ggttcgatat cggttccccc gccggcaaaa ctgacacgta acggggcccg actccggatc
     1621 tccaagcggt ttcctcctta ttccggattg gcacgcgcgt gcgcgcccca gcctgatacg
     1681 ccgattccac caattctgac aaaccggtat ttgcctcaat ttcaataaaa gaccaacttg
     1741 accgggattc gccatgggga ggcatgcttg aatcatcaat ttcggactta agcataggat
     1801 attttatgag actgaagtga agttggtgct ctcatgttaa tccccgaaga agtgggatga
     1861 aacaggttag ggataagctc accttggatg gaacgggaat atactatgct gaaatcccgg
     1921 gaggtggaac agggcgtgaa aggagatcat ccgccggatt ctcaagcggc cgcagggttt
     1981 ctcggccgga aaacagagta tggccgttgg gcgccgctat tagaacgttc ggggttaaaa
     2041 cccaggcgaa tcatgccggt aacagacgca atctatgtcg atacttccga aggctggttc
     2101 cggattggga ggagatccta tccccgagcc gagctgacct ggttacggag tatattagaa
     2161 tatcttgagc aacggtcgtt ccgtggttgg gctgttccct ggcaaaagac gatcatttgg
     2221 gaggagggtg gccattgtta tttggtgcag ccttggcttg atgatagtgg aggctttcaa
     2281 ccggatgacc cggcatccct ggcgcggttc gttgaagttt tgaccgaatt ttaccgttgc
     2341 ggtaaggaat acacccggaa tggtcacgag atttgcaggg accgttttag cagcataccc
     2401 agggaatggg agatggcccg gaaacaactg gcagagtttg agagcgggcg cttttcggag
     2461 aaacaccgca acgaatggcg tgatatgctg aaaaaagccc aggaccgtat cgccctgaac
     2521 ttaaaaaaat gggaagactc gggggccggc ggcatcatcg gtcagcaatc caccaccggt
     2581 gtgattggtc atggaaaact actggccagg tatattgttg ggcgtgatca cgattttgcc
     2641 ttgttgaact gggaacacct ggcttttcaa ccacggattc aagatctggc tacattaatc
     2701 ctcgatctta aattatggga accggaatgg ttgctgtttt tgatcagtga agccgccaag
     2761 gcgcaaccat tttggcctga agaatattcg gcgttattta cgatactctg gcatcctcaa
     2821 gaactgttgg atgcattaaa gaattcagaa gaggatcagg atcgaaaaaa cctcaaggag
     2881 gcggggaaag aaatggccag gaaggaaagg tgtctcacta aggcttggcg tgaattggga
     2941 actgaaaagc gttgggcttt tcgcaatccg cttgccgaaa atctccttcc cgacggaaag
     3001 gtatcatggg tgctgtctcc ggtcgagtct tggggtgata cgggaggcgc gatggattcg
     3061 ttgattcggg tgcaaaaccc ggcttatttg cctcctgaag tgatgcaacg gctttcatac
     3121 ggcctcccgg accggattgt cggcgggggt gatgccgatg ttcccaaagc ggcgaatgcg
     3181 gagatggatc gggttgccgg ttattccgta tcggacttcc agacaagtca aactgttccg
     3241 gcagaggccg caaatttatc atctccggaa aaatccgaac ctgttatacc ggttcaacac
     3301 caagaggcag cagaaacccc tgtggaaccg ccacaaccga atgatatgcc tcggcatacc
     3361 gtgacggcgc caagctccac gcagaccctg cgctgggcaa atttccccaa aaaacagggg
     3421 gccgcgaaac ttgatcaaaa atgaattcga attgtatcaa aaagtggccg gcattgatcc
     3481 cgaaaaccgg atgatgggtc tggcgcctgc cccgccaatc cggatggaac agtttgcgcc
     3541 ggacggctgg aggtgggaga taaccggggg ggtaatggcg ttatggacct ttcatgggtc
     3601 cgagggggct ttggcatatc agcatcagat gctgaaagct ttcgagtctc aaaacacccg
     3661 gggtttttta tatccgttac cgttaagcga tggtcggacc tatgcgcagt tgggcgaaag
     3721 gcaatggttc tattttacat ccctgccccc tcccgggagg gtatcctttg gtaatctcaa
     3781 ggatctagag agcgttgttg agcttctggt ggattttcga aaggcttgcg agcgaggcgg
     3841 gatttggtgc tgcctaccgg agcgtaaaac cacaagtaat ctactccacc gttttcaggc
     3901 catcttggaa catttaaaag tctttgcagc cctcggcaag catcgattac gtcccacctg
     3961 gtttgaccgg attttcctcc aacggttacc agaagctgaa tcccaggcga tagaggctat
     4021 ccgctatttg gaaaccactc gctattttga attatgggaa agacttaccg gtcaggatct
     4081 gatcttgaat aaactggtac ggagtaatct gcggcttacg ccgaagagag aggccgtttg
     4141 tttacgggtg catgatttca gctgggatcc gcccattatc gatctggcca atctgttggt
     4201 taagaccgga cgttccgctc attggacgcc aaagtggttt tcgggtgtta ccggtaaata
     4261 tcgtgagagt ttttccatct ctgatacgga atgggaggtg attaaggctt ttcttttatt
     4321 cccctggagt ttttaccgtt tggcatctcg gtattattac aatcgtaccg aatggtccca
     4381 tgaaactttc atcacgaaat tggaacgttt atgggatgac gaaccgaatc ggcgccaatt
     4441 tttagatgaa ttattgaaaa agggtgggtt aatctaagtt gttatcacga aacacacatt
     4501 ggcaatttgt cggagggtat acaaaattta ctggtaaatc ttccagatac ttggtaattg
     4561 tcggatttga tacgttcttc tcacaatacg ataggtgtga atttttctag aatatcaagg
     4621 ggattgagaa aaatttaata aaaatcctgg caaagtaatt gacaggcttt taaaaacctg
     4681 ttacaatata ttgccttgac attgcttgcc tccaggagta caataaaatt gtaaactcat
     4741 tgaggaggac aaaatgttta acatcggcga taaggtagta tatccaatgc acggggcagg
     4801 tgttattgag agtatcgagg agaaggagat ctccggcgaa aagctccgtt attatattat
     4861 taagttgccg atgggcgaga tgaaggccat ggtccctatg gataaggtca aagaggtcgg
     4921 attacggcaa gtcatcgacg aggaagagat tcttaaggta tttgaggtct taaaatcgga
     4981 atcttcttca atgccgctta attggaatca ccgttatcgg gcgaatctgg aaaagataaa
     5041 gagcggcagc atctatgagg tcgccgaggt ggtacgcaat ttagcatcgc gtgaccggca
     5101 aaaaggttta tccaccggag aaaagaagat gctggagaat gccaggcgga ttctcctgag
     5161 cgagttggta ttggctaaaa atgttaccga aacgacaatg gttgagattt tggaaggtta
     5221 tttgaattga ggcttaggta agtctccata tgtcaatagt aaagcaaggg ttgtcctgaa
     5281 tgttatcggg gcggcccttt tttattgtac tcttctgtga attacgggta tttcgcagtc
     5341 acggccctaa cccccttggc taacatccgg gtctgcgtac acggtacgcg cgccctcccc
     5401 cataacgcta tcaaaaagcg atagttatgg gggagggcta ctgacttgaa tcggcctgat
     5461 gggagagggt tcggataatt gtggaagggt ttgtcaatac tataaatgat tttctattac
     5521 aacgcgaatc aaggaggtga tgatttgtcg aagccaagta taatcggatg gggattcatc
     5581 gttggtggct tattgggtgt ggtttttgta ttatcagttc ttcccgccgc cggctggcaa
     5641 acctggctga ttgcgggtct ggtcggggct gtgataatcg gggcgataac cgtagcggct
     5701 cagaatctta tcaggggaat aattaaaaag accaacggcg aagagttgtt gataaaaacc
     5761 ggcagtttca tatggggttt atttaccacc gcattattgc tgggaatttt taggccgata
     5821 atgaacggta tgtctcagac tttgaattgg acccttacaa tcttattgtt cctcgtatgc
     5881 accggctttt gtgtcggtgt gggttcgctt aaggccaagg agatttgcat gttgattttc
     5941 accgctacag cggctcccgg gcaaaaagcc gtcccgggca agaaccagtt gaaaattctc
     6001 gacaccagtg ccatcattga cggccggatc gccgatctct gcaataccgg ttttttggaa
     6061 ggggtcttga ttattccaaa ttttgtccta aatgagcttc agaagatcgc cgattccgcc
     6121 gatccattac gccgcaaccg g
//
LOCUS       DASVMB010000249         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_694210,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000249 DASVMB010000000
VERSION     DASVMB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694210"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VF531_07340"
     CDS             <1..561
                     /locus_tag="VF531_07340"
                     /inference="COORDINATES: protein motif:HMM:NF020902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HEX7713822.1"
                     /translation="EREFDYYTAVDDLVGDEVLDDAGAGMIGDTEFNSNCYYMYYNLD
                     LDQLIRNLGGITEPVLVAREAAKALLRAFAYTNPAGKQNSFAAHQLPSVIYVELKSDK
                     IPVSYANAFLKPAQATAEKDLVTVSAEKLIAQINETRKYFNDIRVEKRIWLAPAGLAG
                     RPEEAEVAGNIQDLFRIVEDTLREVG"
     gene            562..1239
                     /gene="cas5e"
                     /locus_tag="VF531_07345"
     CDS             562..1239
                     /gene="cas5e"
                     /locus_tag="VF531_07345"
                     /inference="COORDINATES: protein motif:HMM:NF021240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HEX7713823.1"
                     /translation="MANPWLLLLRLEGPLQSWGERSRWGYRDTAMMPTRSGIIGILAC
                     ALGTRRFDPAILELENGLSIGVRADRPGQVIIDYQTISGTIRTADRKQRGGQSGESTL
                     QSYRQYLQDAAFFVVAGGSEELLRRCATALQHPVWPVFLGRKSCIPTRPVYEDLTTEY
                     ATIREALEYHPLTLLGSRGECFCCEVESPAGNIMRRDRLTNLPARVFLERTIQTFSAN
                     PPRSVER"
     gene            1236..1925
                     /gene="cas6e"
                     /locus_tag="VF531_07350"
     CDS             1236..1925
                     /gene="cas6e"
                     /locus_tag="VF531_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HEX7713824.1"
                     /translation="MIYLSRIELNSENPQRRRLLADCYELHRSIMSAFPDRADSVFRA
                     CARDEERGGSDLCGGARRRFNVLFRLNDTGSPVELIVQSDIEPDWEKSIVAPCFPGET
                     CCLKETGEIIENAVENGKVFSFHLRACPSKRLMGNGQKGKRVLLSGCDDQLDWFVRKG
                     SQHGFDVVPESLTLASPQSKVGRNKGIFFQSVDYSGRLMVTDKKVFVHALAGGIGAEK
                     AFGCGLLMISR"
BASE COUNT          672 a          623 c          830 g          679 t
ORIGIN      
        1 gagcgtgagt tcgattatta tacggcagtg gatgatttag ttggcgatga agtactcgat
       61 gatgccggtg ccggcatgat cggcgacacg gagttcaatt ccaactgtta ttatatgtac
      121 tataatctcg atctggatca actcatccgt aatctggggg gtatcacgga gccggtctta
      181 gtggccagag aggcggctaa ggcattatta cgcgcttttg cctataccaa tcctgccggg
      241 aaacagaaca gtttcgctgc gcaccagctg ccgtccgtga tttacgtgga gttgaagtcg
      301 gataagatcc cggtcagtta tgccaatgcc tttttgaaac ccgcccaagc aacggcagaa
      361 aaagatctgg tcacggtaag tgcggagaag ttgattgctc agatcaacga aacccggaag
      421 tatttcaatg atatccgggt ggaaaaacgg atttggctgg ctccggcggg tcttgcgggt
      481 cgaccggaag aggccgaagt ggctggtaat atccaagact tgtttcgaat cgtagaggac
      541 accctgcggg aggtgggcta attggccaat ccctggttgc tcctgttacg gctggaaggc
      601 cctttgcaat catggggtga gcggtcccgc tggggatatc gcgataccgc gatgatgccg
      661 acccgatcgg gaattatcgg aatactggct tgcgccttgg gaaccaggcg tttcgatccg
      721 gcaatcctgg aacttgaaaa tggcttatcc atcggcgtcc gggcggaccg gcccggacag
      781 gttatcatcg attatcagac gatcagcggt accatacgca ctgcggatcg taaacaaagg
      841 ggtggtcaat caggagagag caccctccag tcctacaggc aatatttgca ggacgcggct
      901 tttttcgtcg tggccggcgg cagtgaggaa ttattacggc gttgtgcgac ggcactgcaa
      961 cacccggtat ggccggtttt cttgggtcgt aagagttgta tcccgacccg acccgtttac
     1021 gaggatttga caacggagta tgctactatc cgggaggcgt tggaatatca tcctctcact
     1081 ttgcttggga gcaggggaga atgtttttgt tgcgaagttg agagtccggc cggaaatatt
     1141 atgcgccggg acaggctgac caatcttccg gcccgggtct ttttggaacg gaccattcaa
     1201 actttttcgg ccaatccacc acggagcgta gagcgatgat ctatctttca cgaattgagc
     1261 taaactctga gaacccgcaa cgacggaggt tactggcgga ctgctatgaa ttgcaccgta
     1321 gcatcatgtc ggcttttccg gatcgggccg atagtgtttt tcgtgcatgc gcccgagatg
     1381 aggaacgcgg cggaagtgat ctctgtggag gcgcccgccg cagattcaat gttcttttcc
     1441 ggttaaacga taccgggagt ccggtagagt tgatcgtaca gtcagatatt gagcccgatt
     1501 gggagaagag catcgttgcc ccctgctttc ccggggaaac ctgttgtttg aaagagactg
     1561 gtgaaatcat tgagaacgct gtggaaaacg gcaaggtttt ttcctttcat ttgcgggcgt
     1621 gcccgagcaa gaggcttatg ggtaacggtc aaaaggggaa acgggtgttg ctttcaggct
     1681 gtgatgacca attggactgg ttcgttcgga aaggaagtca acacggtttc gacgtcgtac
     1741 cggaaagcct gacattggcg tcgccccaga gcaaagtggg gcgaaataaa gggatcttct
     1801 ttcaaagtgt ggattattcg ggaagattaa tggtaactga taaaaaagtg tttgtccatg
     1861 cgctggcggg ggggattggt gctgagaagg cattcggatg cggccttttg atgatttcca
     1921 ggtgagttcc tcggtccccg catccgtggg gttccccaca tttttgtggg ggtgtaccgt
     1981 ttaatggagg agttgagaca gaagtattcc ccacagatgt gggggtgtac caaaaccgct
     2041 gaaagcgttg ggattaaaat tcctcacatc agtgggggta tctctgggtt tgtcagaggc
     2101 ggccgacgac ctatctctgc gaggtcgcgc tttgctaagc gcgcggggca agaggtaggt
     2161 aacatgcgaa gccgccaaaa gtcatcggca gttcaaaatt ctattgacaa tctgcggtta
     2221 cttaaaatac aataattact aataactgaa ttattctcaa agacgatgat gaaggaaagt
     2281 accggatccg taacgtttcc tagagagaag gggtcaccgg ctgcaagccc tttcgaaacc
     2341 ggggtccggg aaagttccct gcggagtcga tggctgaacc ttttagtagg ctgttccgga
     2401 attcccgccg ttacccggga tagggcatcg cgtttccgtg ccgtgcctga aataagcgag
     2461 gcgtacaatg caagccatac gtcggtcggc cgatatgcca aacagcttta gctgtttgaa
     2521 cgcacaacat aagccgaccg gaggtcagct tatgttgtgc gactaataag ggtggtaccg
     2581 cgaatctagc ccacgttcgt ccctaagggg gaatgaacgt gggctaattt attaaatgga
     2641 ggcgtaacaa tgattattgt aactagcagc ggtatcaccg gagaacaatg ggataactta
     2701 tgtcaaaagc ttgcggaatg gggatttgaa cttcatgtga ttcaagggat cgagaaacgg
     2761 gtcatcggtg ccgtgggaga taaacgcaag gtagactttc attt
//
LOCUS       DASVMB010000250         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_620028,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000250 DASVMB010000000
VERSION     DASVMB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620028"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF531_07355"
     CDS             <1..360
                     /locus_tag="VF531_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7713825.1"
                     /translation="YISFKEHLTVDQTLNELRRLKPESDTIYYLYVVDDEERLTAVLS
                     LRDLVVAEPWTRLDQIMNRKIIFVYDTDPINSLVEIISKYSLLAIPVVDREMKMMGMV
                     IIDDVVYELLKPKKRKL"
     gene            377..1636
                     /locus_tag="VF531_07360"
     CDS             377..1636
                     /locus_tag="VF531_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7713826.1"
                     /translation="MVAASVKKKAFWGNILIFLSILGPGIITGSVDNDVGGITTYSLA
                     GAKYGYHLLWTLIPAFIVLIVVQEMNARMGIVSGKGLADLIRENAGVKTTFFIFMGLL
                     IADIGNTTSEFAGVAQSMQVFGISKYIAVPLVALLVWLLVVKGNYRFTEKVFLIFSVF
                     LFSYVVSAILAKPNWHEIGQAMIRPRVQFDFDYVSIVIGIIGTTIAPWMQFYMQSSVI
                     EKGLKIEEYKFTIWDVVIGCFATVVVAFFIMVACASTLHVNHIPIREAKDAALSLKPL
                     AGTFASQIFAFGLFVASIFSATILPVATAFYVCEAFGFEAGIDKTWHEAPQFYWLYTG
                     ILIISTLIILLPNAPLLTIILWSQVINGILLPVVLICMMLLVNNKEIMGEHVNRPLNN
                     WIGWITVGVLIVLTFLLLINSLSFIHF"
     gene            1851..2378
                     /locus_tag="VF531_07365"
     CDS             1851..2378
                     /locus_tag="VF531_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEX7713827.1"
                     /translation="MGFIFNEVFWGVLLILLGAAVIIKVVFNINVPVIRIVLAFFLIY
                     LGIRLMIGWGVHQEKPNNTVLFNDSRVEATNSAEEYNVIFGRGTIDLTKLVPNDTAVA
                     RVNVIFGGGVMRIDPAQPVWIEVNSAFGEARMPGGNNISFGKYIYKTQGYQEGKPAVR
                     IEANVVFGGLEIVER"
     gene            2745..3962
                     /locus_tag="VF531_07370"
     CDS             2745..3962
                     /locus_tag="VF531_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7713828.1"
                     /translation="MERVSILKTNSYEDLVAKLEKLLEPVGGWEAYCKSGDRVLLKPN
                     LIMARPVAAAVNTHPALILAVAKLLRDMGCQVAVGDSPGIGSAEGVLRQLGLLSELEA
                     CGVQVVELATPGPVMRGAAMFSGRFKDLRLAKELNEFDRIINLPKLKSHGQMGITLAT
                     KNLFGCVAGPAKGQWHYTVGRNYTNFARLLVEIAFAVRADLHIIDGIVGMDGNGPTHG
                     RARKLDLLIAGTNPIAVDRVAVAALQLNPAQFPIFTAADSLGISGVEMAEIELSGTPF
                     ASCLVDDFQIPALSGLELLFRGHSGLNRMVASLIRRRLVLDPKICIDCRKCEKHCPAG
                     AIRFRKPGPAGQLLAHQDPERVRMHIDPQICIRCCCCQELCPVGALSIRDPLALKLIQ
                     RVINSGGKKSPSQ"
     gene            complement(3956..>4759)
                     /locus_tag="VF531_07375"
     CDS             complement(3956..>4759)
                     /locus_tag="VF531_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713829.1"
                     /translation="VMVFLLFRETTRPFLARVRAKQTENLIATIPARSKENQRVIFVT
                     SYTTDDFIQPAPRITTRAFLTILISLEAAVILLQVLAWVFGSRILSFLSLIPIAVTLG
                     MIFFPKQREPQDPSLDNCGILLELGSILVKARPLSTTVSFFFCGSRSLNSGVQKLPQF
                     LKGGPSLTYIVDLINLNDKRINIVTADGGVISKPSESLLVETLMEVGREKKLPTQEFK
                     FNQITEAYPLKFRGFKTVSVTNPLSGYAGDAPDQDLRELLIGLIRKLDH"
BASE COUNT         1131 a         1066 c         1272 g         1290 t
ORIGIN      
        1 tatatttcct tcaaggaaca tctgactgtt gatcaaaccc tgaacgaact gcgacggctc
       61 aaaccggaat cggataccat ttactatctt tatgtggtgg atgatgagga gcggttgaca
      121 gcagttcttt ccctccggga tctggtggtg gccgagccgt ggacccggtt ggatcagatt
      181 atgaaccgta aaatcatctt tgtatatgat accgatccca tcaactcttt ggttgagatc
      241 atctccaaat acagtctgct cgccattcct gtagttgacc gggagatgaa gatgatgggt
      301 atggtcatca tcgatgatgt ggtttatgaa cttctcaaac caaagaaacg gaaattataa
      361 aagggggtga atgccgatgg tcgcggccag cgttaagaaa aaggctttct ggggtaatat
      421 attgatcttt ttgagcattt tagggccggg gatcatcacc ggaagtgtag ataacgacgt
      481 cggcgggatt accacctatt cgttggccgg tgcaaaatac ggctatcacc tgctttggac
      541 tttaatcccg gcatttatcg tcttaatcgt agtccaagag atgaatgcca ggatgggaat
      601 cgtctccggc aagggtttgg cggatctgat ccgggaaaac gccggggtga agacaacctt
      661 tttcatattc atgggcttat tgatcgcaga tatcggcaac accacctctg aatttgccgg
      721 ggtagcccag agcatgcaag tctttggcat cagtaagtat attgcggtgc cgctagtggc
      781 gctcttggta tggttactgg tggtcaaggg aaactatcgt ttcaccgaaa aggttttctt
      841 gatcttcagt gtgtttctct tttcttatgt cgtatcggcg atattggcca aaccgaattg
      901 gcatgagatc ggacaggcaa tgattagacc ccgggtgcaa ttcgacttcg attatgtctc
      961 gattgttatc ggcatcatcg gcacaactat tgcaccgtgg atgcagtttt acatgcaatc
     1021 gtcggtaatt gagaaaggcc tcaagattga ggagtataaa tttaccatct gggacgtagt
     1081 catcggctgc tttgctacgg tagtagtggc tttcttcatc atggtcgcct gtgcctcgac
     1141 acttcatgtc aatcatattc cgatccgtga ggctaaggat gccgcacttt ccctgaaacc
     1201 actggccggt acctttgcct cccagatctt tgccttcggt cttttcgtcg catcgatctt
     1261 ttcggccacg atcctgccag tagctaccgc cttttacgtc tgtgaggcat tcggctttga
     1321 agcggggatc gacaagactt ggcatgaagc accgcagttt tattggctct acaccggaat
     1381 tttgatcatc tccacgctga ttattctact ccccaatgcg ccgctcttga ccatcatcct
     1441 gtggtcgcag gtgattaacg ggattttgct cccggtagtg ttgatctgca tgatgctcct
     1501 ggtgaacaat aaggaaatca tgggagaaca tgtgaaccgg ccgctgaata actggattgg
     1561 ttggattacg gtgggggtat tgatcgtatt gaccttctta ttgctcatta attctctttc
     1621 gttcatccat ttttagcttc tcgatccgcc gttctagatt cccctctgtc atagaaggaa
     1681 atttcgacgc aggagatcgt aaagatgagc cgaatttata aaaggccggt atttatggga
     1741 tacacccgca tcaatgcttt acaccgtttg aaggagtcct gcataaattc ttggtggagt
     1801 tgaacggcgt ttcaggattg gccggagatt acggaggtgg aggtatgagg atgggtttca
     1861 tttttaatga ggttttctgg ggtgtacttt taatcttgtt aggtgcagcg gtcattatca
     1921 aggtggtctt caacattaat gtcccggtta ttcggattgt tctggctttt ttcctgattt
     1981 atcttggaat tcgattgatg attggttggg gtgtccatca ggaaaagccc aataatacgg
     2041 tattgttcaa tgacagccgg gttgaggcga ccaattccgc cgaggagtat aacgtgatct
     2101 tcgggcgtgg aaccattgat ctaacgaaat tagttccaaa tgacaccgct gtagccagag
     2161 taaatgtgat attcggcggc ggtgtgatgc ggattgaccc tgctcaaccg gtttggatcg
     2221 aggtcaattc ggcttttgga gaggcgcgta tgcccggcgg caacaatatc tcctttggaa
     2281 agtatattta taaaactcaa ggttaccagg aaggtaaacc ggcggtacgc atcgaggcca
     2341 acgtcgtatt cggcggactg gagatcgttg aacgttagcc atgaggcatg agccggaagg
     2401 gaacaggcaa taggcaacag ggaaaagtga aaaattataa gctaaatttt aagaggcaaa
     2461 gcgcctcttt tttattttga ctacttcaca cgaactatat gtggtcgact ggtttaccac
     2521 ttatgattcc tcctcaaccg ctaccggcag gtagtgaagg gtgtaaccct ttggcatccg
     2581 ggtggtgagg gccctcaaat ctgccatgaa ttgcagcgct ttaaaactta gattcggcaa
     2641 ttctctttac cccaaatcca ttccatggta tgattcaatg agacgcatgt accaatcctt
     2701 ttcagagctt ggctgcgacc catttttttc gaaagaggtt aaccatggaa cgggtatcta
     2761 tcttaaaaac gaattcttat gaagatctgg ttgccaaatt ggagaagctc ctcgaaccag
     2821 tgggaggatg ggaggcttat tgcaaatccg gcgatagggt attgcttaaa ccaaatctaa
     2881 tcatggcgcg acctgtagcg gcagctgtca atactcatcc ggcactgatc ctggcagtgg
     2941 cgaagctcct gcgggatatg ggttgtcagg tggcggttgg ggacagtccc ggaatcggta
     3001 gcgctgaagg ggttctcagg caattgggct tgctttcgga gcttgaggcc tgcggggttc
     3061 aagtggtgga attggcgacc ccggggccgg ttatgcgcgg tgccgcaatg ttttcggggc
     3121 ggttcaagga cctgagactc gccaaagagc tgaatgaatt tgaccggatt atcaatctac
     3181 cgaaattgaa atcccatggt caaatgggga ttaccctggc caccaaaaat cttttcggtt
     3241 gcgtagccgg cccggccaag ggacagtggc attacaccgt gggacggaat tataccaatt
     3301 ttgccaggtt actggtggag atcgcctttg cggtccgggc agatctgcat attatagacg
     3361 ggattgtggg aatggacgga aacggtccga cccatggccg tgcccgtaag cttgatttgt
     3421 taatcgccgg aaccaacccg atcgcggtgg atcgggtggc cgtagcggcg ttacaattga
     3481 acccggctca gtttccgatc tttacggccg cggattcctt gggcatttcc ggagtggaaa
     3541 tggccgagat tgaacttagc ggtaccccgt tcgcctcctg tttggtagat gatttccaga
     3601 taccggcctt atcaggacta gagcttcttt ttcgaggaca ttccggtttg aaccggatgg
     3661 ttgcctcgtt aatccggcgc cggttggtgc ttgatcccaa gatctgcatc gactgccgga
     3721 agtgtgaaaa acattgcccg gccggggcaa tccggtttcg aaaaccgggc cctgccgggc
     3781 agctgcttgc tcatcaggat ccggagcgtg ttcggatgca cattgatccg cagatctgca
     3841 tccggtgttg ctgttgtcag gaactctgtc cggtgggagc attgtcgatc cgtgatcctt
     3901 tggcgctaaa gctgatccaa agggtaatca acagcggcgg aaaaaagtct ccgtctcagt
     3961 gatccagttt ccggataaga ccaatcagta gttctctgag atcttgatcg ggtgcgtcac
     4021 cggcgtatcc ggaaagcgga ttggtaacgg aaactgtctt gaatcctcgg aatttcaagg
     4081 ggtaggcctc ggtaatctga ttaaatttaa actcctgtgt aggtagtttt ttctcgcggc
     4141 caacctccat taatgtctct accagcaggc tttcactagg cttggaaatg acaccgccgt
     4201 cggcagtgac gatattgatc cgtttatcgt tcaagttgat caaatccacg atgtaagtaa
     4261 ggctcggacc gcctttcaaa aactggggta gtttttgaac ccctgaatta agcgaacggc
     4321 tgccgcagaa gaagaatgat accgtggtgc tgagaggccg ggcttttaca agaatagaac
     4381 ctaattccag taagatcccg cagttatcca gactgggatc ctgaggttcc cgttgtttcg
     4441 gaaaaaagat catccccagg gtgacggcga ttggtatgag agacaggaag ctcaagatcc
     4501 gggagccgaa aacccatgcc agaacttgca gtagaataac agccgcttcc aaactgatta
     4561 agatggtcaa aaaagctctg gtcgtgatgc gcggtgcagg ttgaataaaa tcatccgtag
     4621 tatagctcgt tacaaagatt acccgttggt tttccttact ccgggcggga atggttgcga
     4681 tcaggttctc ggtctgtttg gcccggactc tcgccaaaaa aggccgcgtt gtctccctaa
     4741 ataataggaa caccattac
//
LOCUS       DASVMB010000251         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_324658,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000251 DASVMB010000000
VERSION     DASVMB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324658"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VF531_07380"
     CDS             complement(<1..1501)
                     /locus_tag="VF531_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HEX7713830.1"
                     /translation="MNYNRRLALLREKKIEHTQAKVRAAGFIDEDDYGTVPVPEDFHW
                     QSIPNHPNGSFYGASGWAANFKSLMEIHPVYVDPLDALAGRWSIIMPKMRKQDWLPEH
                     DTSFLREEQLKYDIDPGIGASSHFGPDYRIGLELGWDGLLAKVRHYRGLYGPEKSEFY
                     QAEEDVILAVQTWIHRTIAHIHELEKTEPHPGLRANLEQMAAVNEWVIGKPPRTLREA
                     CQWIAWFNMVSRTYNRDGAGGQLDALLRPYYEKDIAAGLIEDEDAIFYIACLLLNDPH
                     YYQLGGPGPDGQDVTSHISFLILEAAHRLDSSNNLTVRVHDGLNREFFLKSVQYLFED
                     RKGWPRFAGDKGLVEGFMRNGYPVELARERIAVGCNWMAIPGREYTMNDCVKLNAAKV
                     FDVAFHEMMDDTGVEPSLDELWRCFESHFNRAILCTSKGIDLHLRHQKENAPELMMNL
                     LCHGPVEKGLDVSDGGVEYYNMCIDAVGLATVSDSFAALEQRVAIEKALT"
     gene            complement(1565..2470)
                     /locus_tag="VF531_07385"
     CDS             complement(1565..2470)
                     /locus_tag="VF531_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEX7713831.1"
                     /translation="MEGYVTEIQRFSVHDGPGIRTTVFLKGCNMRCIWCHNPETLNPG
                     PELQLFPEKCIGCGECFKCCPRQAHLVIAGERKLRRELCESCGKCAGGCYADALVMVG
                     RKMSPAQVLDEAMEDKEFYASSGGGVTVSGGEPVYQADFSHEILRLCKEKGIHTAIES
                     NMAWPWEKVAPLLPVTDLVMMDLKVLDDQMHQRVTGISNRAVLDNAKKLSRQSLPMII
                     RTPIIPGVNDTVQAIAAIAEFIAGFTHLLYYELLPYHPLATGKYHSLGMKYGMEGVKR
                     PERALMDELAAAARSRGVTVRCTGA"
     gene            complement(2559..3536)
                     /locus_tag="VF531_07390"
     CDS             complement(2559..3536)
                     /locus_tag="VF531_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7713832.1"
                     /translation="MTPKERAVAALTLKVPDMVPTFELEFQLEEGMFGRKFITADLEP
                     ENLAKLSWREKEAKIFELAQYTVQVYSSLEYSIIPGYGPGDLDGFWKRGEPLPEELRL
                     FHKYLRELIGDTVMIGFHGDGTFSIPDGNEMYAFAYAIADDPDGMKQKAETMAHAAIR
                     RNRMLAESGVDCLFLCSDYCYNSGPFLSPAHFREFIQPYLYMIIEAARREGLYTIKHT
                     DGNIMPILDQLAECRPHALHSIDPMAGVDIREVKRLVGDKVCLCGNVNCATLQTGTAA
                     EVDLSAEYCLRYGKPGGGYIFCTSNVPFKGMPVAQYRRMLDIWKAMRSY"
     gene            complement(3553..>4044)
                     /locus_tag="VF531_07395"
     CDS             complement(3553..>4044)
                     /locus_tag="VF531_07395"
                     /inference="COORDINATES: protein motif:HMM:NF036773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HEX7713833.1"
                     /translation="IAILNDSKYGVNVSGNSINLTLLRAAQAPDAEADLGQHEFKYAL
                     LSWNGSFLTSPVVRAGHELNCPAVTVRGGAGERSIFTVDEPDVIIETVKPAEDGSPDI
                     ILRLYEAKHTTARCRLICSLPVKTAYRTDMLEKEEEPLMVENSRITLDFRPFEIKTLR
                     LKF"
BASE COUNT          942 a         1174 c          954 g          974 t
ORIGIN      
        1 atgtcagggc cttttcgatg gcgacccgct gctccagggc ggcaaaagag tcggaaaccg
       61 tcgccaaacc caccgcatcg atacacatgt tgtaatactc aaccccgcca tcggatacat
      121 ccaaaccttt ttcgaccggt ccgtggcaca aaaggttcat catcaactcc ggggcgtttt
      181 ccttttggtg cctgagatga aggtcgatcc ctttagaggt gcaaagaatg gcccggttga
      241 aatgagattc gaaacaccgc cacaattcat ccagggacgg ttccaccccg gtatcgtcca
      301 tcatttcatg aaaagccaca tcaaaaacct tggctgcgtt taatttcaca caatcattca
      361 tggtgtactc ccggccggga atggccatcc agttgcaccc tactgcgatc cgctcccggg
      421 cgagctccac cgggtatcca ttacgcatga agccctcaac cagaccctta tcccccgcaa
      481 aacgcggcca accttttcta tcctcaaaca ggtattggac tgattttagg aaaaactccc
      541 ggttcaatcc gtcatggact ctgacggtca gattattgga gctgtcgagc cggtgggctg
      601 cctcgagaat cagaaatgaa atatggctgg taacatcctg gccgtctggg cccgggccac
      661 ccaactgata ataatggggg tcgttcaaga gcaggcaggc gatataaaat atcgcatcct
      721 catcctcgat caatccggcg gcaatatcct tttcatagta cggccgcaac aacgcgtcga
      781 gttgaccgcc ggcgccgtca cggttatagg tgcgcgagac catgttgaac caggcgatcc
      841 actgacatgc ctctcttagg gtacggggag gcttgccgat tacccattcg tttaccgccg
      901 ccatctgctc cagattggcg cgaagcccgg gatgaggctc tgtcttctcc aattcgtgga
      961 tgtgagcgat ggttctatgg atccaggttt gcaccgccaa aatgacgtcc tcttcggcct
     1021 gatagaactc acttttctcg ggaccgtaga gtccccgata atgcctcacc ttggccagga
     1081 gtccgtccca acccagttcc agaccgatcc ggtaatccgg tccaaaatgt gaactggcgc
     1141 caattcccgg gtcaatgtca tatttgagtt gttcctctcg taaaaacgaa gtatcatgtt
     1201 ccggcagcca gtcctgcttg cgcattttcg gcatgatgat cgaccaccgt ccggccagtg
     1261 catccagcgg atccacataa accggatgga tttccattag cgatttaaag ttggctgccc
     1321 agccggaggc tccataaaac gatccattgg gatggttcgg aatcgactgc caatggaaat
     1381 cctccgggac cggaaccgtt ccgtaatcat cttcatcgat aaatccggcg gcgcgaacct
     1441 ttgcttgggt atgttctatt tttttctccc gaagcaaggc aagccgtcga ttataattca
     1501 tttcatcctc tatcatgatg atctcgcccc tttctataga tcttgatcag gcaacgggca
     1561 tcactcacgc ccccgtacat ctgacggtaa ctccccggct cctggcggca gcagccagtt
     1621 cgtccatcaa cgcccgttcc ggacgcttga ccccttccat accgtatttc atccccaaac
     1681 tatggtattt tccggttgcc agcggatgat aaggcaacag ctcatagtag agcaaatgag
     1741 tgaatcccgc aatgaattcg gcaatcgccg caatggcttg gaccgtatcg ttgactcccg
     1801 gtatgattgg agtgcggata atcatcggca gagactgccg cgaaagcttc ttggcattat
     1861 ccaataccgc ccggtttgaa atacctgtaa ctctttgatg catctgatca tcaaggactt
     1921 tcagatccat catcaccaga tcagtgactg gaagtaaggg ggctaccttc tcccaaggcc
     1981 atgccatatt gctttcaatc gcggtatgga tgcccttctc cttgcaaagc cgtaagatct
     2041 catgactaaa gtccgcctga taaaccggct caccgccgga gaccgtaaca cccccgccgg
     2101 aacttgcgta gaattccttg tcctccatgg cttcatccaa aacttgggcc ggactcattt
     2161 ttctgcccac catcaccagc gcatcggcat aacaacctcc ggcacatttg ccgcaggact
     2221 cgcataattc acgcctaagt ttccgctccc cagctatgac aagatgggct tgacggggac
     2281 agcacttgaa acattcgccg cagccaatgc acttctccgg aaacagctgc aattccgggc
     2341 cgggattcag cgtctcgggg ttatggcacc agatgcagcg catgttacat cccttcaaaa
     2401 aaacggtagt ccggattccc ggtccgtcgt gcaccgaaaa acgttgaatc tccgttacat
     2461 acccttccac gtcattcacc gccagttacc tgtctgaagt cgcaaagtcc aaagtcataa
     2521 aggcacaagc ccaaagtcat aagtcaaatc cgcagagatc aatagctcct cattgccttc
     2581 cagatatcga gcattcgccg gtattgcgcc acgggcattc ctttaaaagg aacattgctg
     2641 gtacaaaaga tgtaaccgcc gccaggtttt ccgtatctca ggcagtattc ggcgctcaag
     2701 tcgacctcgg ccgcggttcc ggtttgcagc gtcgcgcaat tgacgttgcc gcacaaacat
     2761 accttgtcgc cgactaacct tttcacttcc cggatgtcca cgccggccat ggggtcgatg
     2821 gaatgaaggg catggggccg gcattccgcc aattgatcca gaatgggcat gatgtttccg
     2881 tcggtatgct tgatggtata aagtccttcc cgccttgccg cctcaatgat catatataaa
     2941 tagggttgaa taaactcccg gaagtgggcg ggtgagagaa acggtcccga attgtaacaa
     3001 taatcggaac agaggaataa acagtccacc ccgctctcgg ccaacatccg gttcctcctg
     3061 attgcggcat gggccattgt ttccgccttt tgcttcattc catcggggtc atccgcgatg
     3121 gcataggcga aggcgtacat ctcatttcca tccggaattg aaaaggttcc gtcgccatga
     3181 aagccgatca tcaccgtatc gccaatcagt tcccggagat atttgtggaa caatctcagt
     3241 tcctcgggga gcggctcgcc ccgtttccaa aaaccgtcca ggtcgccggg gccgtatccc
     3301 ggaatgatcg aatattcgag gctactgtat acctgcactg tgtattgggc cagttcgaat
     3361 attttggctt ccttctcccg ccagctcaat ttggcgagat tttccggctc caaatccgcg
     3421 gtgatgaact ttctcccgaa catcccctct tccagctgaa actccagttc aaaagtgggt
     3481 accatatccg gtacctttaa ggtcaatgcg gcaaccgctc tttccttggg tgtcatctga
     3541 aaaccccctt ttttaaaact tcaaccggag cgtcttgatc tcaaacggtc tgaaatccag
     3601 ggtaatacgg ctattctcaa ccatcagcgg ttcttcctct ttttccagca tatcggtccg
     3661 ataagcggtc ttgaccggta aggaacagat cagccggcaa cgggcggttg tatgttttgc
     3721 ctcgtataac cggaggataa tatccgggga tccgtcctcg gccggtttga ccgtctcaat
     3781 aatgacatcg ggttcatcca cggtaaagat cgagcgttca ccggctccac cccggactgt
     3841 aaccgccggg cagttcagct catgccccgc ccggaccacc ggactcgtta gaaaacttcc
     3901 gttccatgaa agcaatgcat acttgaattc atgttgaccc agatcggcct cggcatcggg
     3961 agcttgggcc gctctaagca gggttaaatt gatactgttg cccgagacat tgaccccata
     4021 cttgctgtcg tttaggatgg cgat
//
LOCUS       DASVMB010000252         6553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_473498,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000252 DASVMB010000000
VERSION     DASVMB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473498"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF531_07400"
     CDS             <1..193
                     /locus_tag="VF531_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713834.1"
                     /translation="DEEVATEDEATSDEEVVAEDEDEATSDEEVVAEDEAAADEEVAT
                     EDEATSDEEVVAEDEDAAE"
     gene            459..1799
                     /locus_tag="VF531_07405"
     CDS             459..1799
                     /locus_tag="VF531_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7713835.1"
                     /translation="MQKKLKALVYISLISIYLSLAALGCPAPNLVSFEPIKGTNTQIV
                     ELTVTGSKFHKSVFVKLSQAGQADIMATDLKILSPTQISCKLDLRGKPAGKWNLVVAN
                     IGTFTKKEKPAVSEGGFTIENPVPTVTSVTPVQAIHNKTIETSITGTNFRPGAIVMLS
                     NGAMDIGATDVNILSDTRISCRLNLNGAALGVYNLKVINDDGQTGVLPNGFTVVKPAP
                     APVPDPVINKLSPDRGFNNGSILVNFEGANFAAGLKVKLSKTGQTDIPGLNIQVGSSS
                     RFSCFFDIAEQPVGKYDVIITNPDGHSATLTEGFTVEVFTPAVDPNKLLKPIYFDYNK
                     WNIKSDQSPVLEKDLAILKDNPNLFILLGGHADERGTREYNLELSSKRANAIKQYLVA
                     QGIAPERITIYAYGKDFLAKTEHTEAAQKYNRRVDILMWEAPPTKEQGIVNPNL"
     gene            complement(2035..2850)
                     /locus_tag="VF531_07410"
     CDS             complement(2035..2850)
                     /locus_tag="VF531_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713836.1"
                     /translation="MQKKIGFSVAFFWIAIIIYLTNICGILLTVILNSIGKGWFSGLF
                     PKIFTTEWYKYVAMDHDIPNLLMVTFVVAVTVVVISLLIAFPSAYALARYDFKFKGII
                     LSLLLLPMIIPPMAYGIPLAALLYKLRLASRITGVIIANLVPIVPFMILILQPFIEQI
                     GTNLESAAKMLGASRIKTFFKVLIPLSKPGIFTASILSIVKTISMFELTFLVAGGKSQ
                     TIIVALYSDAYAAGARPPQAVDALAVIYFITTLTCLLVALKFVSPTQMVFKIK"
     gene            complement(2937..3800)
                     /locus_tag="VF531_07415"
     CDS             complement(2937..3800)
                     /locus_tag="VF531_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713837.1"
                     /translation="MSAPNKWNRSMTVVLLPALTYLAVLFIGPFLYGLYLSFTNANGA
                     FTLANYLRFFTDQWEYRTIGVTLWISLPVTILNVVLAIPFAYYMRHGVQGEKLITFFL
                     IVPITLGTVLIADGMLSYMGPNGWLNQLLMALHIVKTPFQFTHNYLGVIISLFISGFP
                     FAFLMLLGYISGINPDLEKAAQMLGANKVQIFWKILFPLLAPGIAIAFCLNFVMAFSV
                     YPSAVLLGQPSGPTRVIAIAAYEWSFEKFDFNMGTTISMVMAAIELIIIMLVLFWRQR
                     IYKGASIVGKG"
     gene            complement(3797..4933)
                     /locus_tag="VF531_07420"
     CDS             complement(3797..4933)
                     /locus_tag="VF531_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713838.1"
                     /translation="MRDFKTLSIEHVHKSFGNKEALNDFTLTMEKGEFITFLGPSGCG
                     KSTALNCISGLLPITKGTISIDDECIDDSVKQVPPEKREFGMVFQNYALFPHLTVFNN
                     IAFGLQLKKMAPDVIKEKVTNVLKMVHLEGYESKFPSQMSGGEQQRVAIARCIVMEPR
                     LLMLDEPLSNLDAKLRIDMRYELKALHDRLQVTSIYVTHDQQEALALSDRIVVMKKGR
                     IQQIGTPEEIYSDPVNLFVADFMGFRNIWSAKVENYVDNGTDIEITLNVNGTQLVSRR
                     KNSDHVKARNLQIAQAFKDKEEVFTAIRPEDFKLGTGRINELNCQVDVVEYLGQVSQV
                     AAFLDNGTRLDLRPDGKLRQGEKVDAWVEPEKIVVFPREEGENL"
     gene            complement(5023..6195)
                     /locus_tag="VF531_07425"
     CDS             complement(5023..6195)
                     /locus_tag="VF531_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7713839.1"
                     /translation="MKRFRLLVFVLAVTMILSVLTAGLGAESLVSKPVKLSIIDVAGN
                     LQLTKPAIEAFKAAHPDWVSDIEYIKATAPELPAKIKAQQAAGNLDTTMVLTGYDAMA
                     SGVEMGLYEKIYPAYKDKFPNLEKDYLPGAAQAFKMFEGYGITLVFCPGGPMFTYNPD
                     KVKKVPTTAQELLAWAKANPGKFFYARPANSGPGRAFLQGLPYILGDKNPKDPQTWTK
                     TWAYLKELDKYIDYYPTGTGITFKELGEGTRYIIASHLGWDMNQRILGTIPANFKGFF
                     LKKTTWVTDAHFMAIPKGLDNDRKKVTLELMAYLMTPEAQAVTYDDGYFYPGPAIKNV
                     PITMAPKASQDRVKAAERTEYTKAVKDFPSTPQLDSQPMVEAMDIWDRQIGSKLSK"
     gene            complement(6350..>6553)
                     /locus_tag="VF531_07430"
     CDS             complement(6350..>6553)
                     /locus_tag="VF531_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7713840.1"
                     /translation="KKETGENFIDYLTGLRIRKSKEFLANPLSSVKEVCYQVGYKDPN
                     YFARVFKKISGVTPTEYKVNKPH"
BASE COUNT         1873 a         1569 c         1432 g         1679 t
ORIGIN      
        1 tgatgaagaa gtagctaccg aagacgaagc cacttccgat gaagaagtcg tcgccgaaga
       61 cgaagacgaa gctacttccg atgaagaagt cgtcgccgaa gacgaagccg ctgctgatga
      121 agaagtagct accgaagacg aagccacttc cgatgaagaa gtcgtcgccg aagacgaaga
      181 cgctgctgaa taaaaccgaa agtaaaccca accactacac ctgggaaaat cccccaggtg
      241 ttttttattt tccaccaaaa ataacgatta tatttccaaa ccctgaaccc ctatcaagca
      301 gtcccaacta atttataaaa ctttaaaatc gtagaactaa cccatttggt tccccattca
      361 gtttgagacg tgtaaaaata taccaattta gtataaaggg aggaatcgga gatgatgatt
      421 tagaaataac tcatcaaatt ggaatggagg taacaggaat gcaaaagaaa ctaaaagcgc
      481 tggtctatat tagtctaatc tcgatctatc ttagtctggc cgctttggga tgccctgcgc
      541 ccaatcttgt ctccttcgag cctatcaagg gaacgaacac acagattgtt gaattgaccg
      601 tcacgggcag caagtttcat aaatcagtct tcgtcaaact gtcccaagcc ggacaggccg
      661 atatcatggc aaccgatttg aaaattctct ctccgaccca gatcagctgc aaattagatc
      721 tgcgtggcaa accggcagga aagtggaacc tggtggtcgc caacatcggg acattcacca
      781 aaaaggaaaa gcccgccgtg agcgagggtg ggttcacgat tgaaaacccc gtaccgacag
      841 tgacttccgt aacccccgtt caggcaatac ataataaaac catcgaaacc agtattaccg
      901 gtaccaactt ccggcccggg gcgattgtta tgttaagtaa tggggccatg gatattggcg
      961 ccacagatgt taatattctc tccgatactc ggatcagttg tcggcttaat ctgaatggag
     1021 ccgctttggg agtttataat ctgaaagtca tcaatgatga cggccagacc ggggtattac
     1081 ccaatggttt cacagtcgta aaaccggcac cggccccggt tcccgaccca gttattaaca
     1141 aactctctcc ggatcgcggt tttaacaatg gttcaatctt ggtaaacttt gaaggcgcaa
     1201 actttgcggc cggattgaaa gttaaactct caaaaaccgg ccaaaccgat attcccgggc
     1261 taaacattca ggtgggatct tcatcccgtt tctcttgttt ctttgatatc gcagagcaac
     1321 ccgttgggaa atacgatgtc atcataacca acccggatgg tcattccgca actttgacgg
     1381 aaggttttac agtagaggta ttcacccctg cggttgatcc caataaactg ctcaagccaa
     1441 tctatttcga ttataacaaa tggaacatca aatcagacca gtcaccggtt ttagagaagg
     1501 atctggcgat cctgaaggat aaccccaatc tctttattct gctgggtggc catgccgacg
     1561 aaaggggtac ccgcgaatac aatctggagc tttcatccaa acgggccaac gccatcaagc
     1621 agtacctggt ggctcagggc atcgctccgg agcgaataac catctatgct tacggcaaag
     1681 attttctggc caaaaccgaa cataccgaag cggctcagaa atataaccga cgggtcgata
     1741 tcctgatgtg ggaagcgcct cctaccaaag aacagggaat tgttaatccg aatctctaat
     1801 acgatttttt ggagagaatt cgcatttttc gtttcactac cagtttgttc gtaataaata
     1861 acccccgaat tcgggggtta tttatgtcct atttataaaa ctattgcaca tactgcactt
     1921 gctttcaata tttggatcaa ccgtttctaa aaacaaaatc ctatctttat atagacaaaa
     1981 gaggccgact tttagcccgc ctctcttgat aaaatttaat ccccaagtta tcttttactt
     2041 tatcttgaac accatctggg tcggacttac gaattttagg gctaccaaca gacaggtcaa
     2101 ggtggtgatg aagtaaataa ctgccagtgc gtcaacggcc tgagggggtc ttgctccggc
     2161 ggcataagca tcggaataaa gcgcgactat gatggtttgc gatttaccgc cggccaccag
     2221 gaatgtcagc tcaaacatag agattgtttt gacgatcgaa agaatactgg ctgtaaagat
     2281 cccgggcttc gacagaggaa tcagaacttt aaagaaagtt tttatcctac tggctcccaa
     2341 cattttagcg gcggattcca gattcgtccc gatctgttca ataaaaggct gtaaaattaa
     2401 gatcataaac ggcacaatcg gcaccaggtt ggcaatgatt accccggtaa ttcttgacgc
     2461 caaccttaac ttgtacagca gggcggccaa aggaatacca tatgccatgg gagggataat
     2521 catcggcaat aatagcaacg ataaaatgat gcctttaaat ttaaagtcat atctggccaa
     2581 ggcataagcc gacgggaaag caatcaggag cgagataacc actacggtaa cggctaccac
     2641 aaaagttacc atgagcagat tgggaatatc atggtccatc gcaacatatt tataccattc
     2701 cgtcgtgaaa atcttcggga ataatccgga aaaccatcct ttaccgatag aattcaggat
     2761 aacggttaac aagataccgc agatattcgt caaatagatg atgatggcga tccaaaagaa
     2821 agctactgaa aaaccaatct ttttctgcat tctattagtc ctccattcaa acaaaaatta
     2881 taccggcgga aaacgccgag agtttgtgat cccatacttc tgaaatccgg gacggctcaa
     2941 cccttcccga cgatggatgc gcctttataa atccgttgtc tccaaaagag gactaacata
     3001 atgatgatta attcgatagc cgccatgacc atggagatgg tggtacccat attaaaatcg
     3061 aatttttcaa aggaccattc atacgccgca atcgcgataa cccgggtggg accggatggc
     3121 tgtcctaaca ataccgctga tgggtaaact gaaaaagcca tgacaaaatt gagacagaat
     3181 gcgatggcaa tccccggagc cagtagtgga aatagaattt tccagaagat ctggacctta
     3241 tttgccccga gcatttgggc ggctttctcc aggtccggat tgataccgga gatatacccg
     3301 agcaacatca ggaacgcaaa gggaaaaccg gatatgaaca aggaaatgat tacaccgaga
     3361 taattatggg taaattgaaa cggggttttg acgatatgca aagccattaa caattggttc
     3421 agccaaccgt tcggccccat atacgacaac atgccgtctg cgataagcac cgttcccaaa
     3481 gtgatcggaa cgatgagaaa aaatgtgatg agtttctcac cctgaacgcc gtgacgcatg
     3541 taataagcaa acggaatcgc gagcacaaca tttaatatgg tcaccggcaa gctaatccac
     3601 agggtgaccc cgatggtccg gtattcccat tgatcagtaa agaacctgag ataatttgcc
     3661 aatgtaaaag cgccattggc attggtaaaa ctgaggtata aaccgtacag aaacggtccg
     3721 atgaacaaaa ccgccaaata agttaacgcc ggtaataata ctaccgtcat actgcgattc
     3781 catttattcg gggcactcat aaattctccc cttcttccct gggaaatacc actatctttt
     3841 cgggttcaac ccatgcatca actttttcac cctgccttaa cttgccatcg ggacggagat
     3901 ccaagcgcgt gccgttatcc aagaaagccg cgacctggct gacctgaccc agatattcaa
     3961 cgacatcaac ctggcaattg agctcattta tccggcccgt gccgagctta aaatcttcag
     4021 gccggatagc ggtaaagact tcctctttgt ccttgaacgc ctgcgcgatt tgaagattac
     4081 gcgctttgac atgatcggaa tttttacggc gcgagaccag ctgggtcccg ttcacattta
     4141 acgtaatttc gatatccgta ccattatcga cataattttc gactttggcg gaccaaatat
     4201 tgcgaaatcc cataaagtcc gcgacaaaaa gattaaccgg gtccgaataa atctcctccg
     4261 gagtcccaat ctgctgtatc cggccctttt tcatgacaac gatccggtcc gaaagcgcca
     4321 gagcctcttg ttggtcatgg gtgacataaa ttgaagtaac ttgtaaccga tcatgcaggg
     4381 cctttaactc gtagcgcata tcgatacgca attttgcgtc aagattggag agcggttcat
     4441 ccaacatcaa cagccgcggt tccattacga tgcagcgggc aatggcgacc cgttgttgct
     4501 caccacccga catttggctg ggaaatttgg actcgtaccc ttccagatga accattttta
     4561 agacattggt gactttttcc ttgataacat cgggagccat ctttttgagt tgcagtccga
     4621 aagcaatatt attgaaaacc gttaaatggg gaaacaaggc ataattctgg aaaaccatcc
     4681 cgaactcccg tttttcggga ggaacctgtt tcaccgaatc atcgatgcat tcatcatcga
     4741 tggaaatcgt ccctttggta atgggtaata gtccggagat gcaattcagg gcggttgatt
     4801 taccgcagcc ggaagggcct aaaaaggtaa tgaactcccc tttctccatg gttaaggtga
     4861 aatcattgag cgcttccttg ttaccgaatg atttatgaac atgctctatc gacaatgtct
     4921 taaagtctct catctttaat caccattctg ttttttttta ggataaatgg acgctagctt
     4981 cttaaacagc caacgtccat ttatccctcc ttaattcaag tattacttgg aaagcttgga
     5041 gccgatctgt cgatcccaaa tatccatggc ttcaaccata ggttgcgagt ccaattgagg
     5101 cgttgacgga aaatccttta cggctttggt atattcagtc cgttccgcgg ctttaaccct
     5161 atcttgactt gctttgggag ccatggtgat cggcacgttc ttgatcgccg gtcccggata
     5221 gaaataaccg tcgtcatagg taacggcttg cgcctcaggg gtcatcaggt aagccatcag
     5281 ttccagggtg actttcttgc gatcattgtc cagccctttc ggtatagcca taaagtgagc
     5341 atccgttacc caggtggttt tcttcaggaa gaagccttta aagttggcgg gaatagttcc
     5401 caaaatacgt tggttcatat cccaacccag gtggctggcg ataatatagc gggttccttc
     5461 tcccaattcc ttaaaggtga taccggtccc ggtcggataa taatcgatgt atttatcaag
     5521 ttctttcaga tacgcccagg tctttgtcca agtctgcggg tctttcgggt ttttgtcccc
     5581 taagatatac ggcaagcctt gtaagaaggc gcggcccggt ccggagttag ccggtctggc
     5641 ataaaagaat ttcccggggt tcgctttggc ccaagccaga agttcttgag cagtggtcgg
     5701 aactttcttg actttatcgg gattataggt gaacattgga ccaccagggc agaagacgag
     5761 agtaattcca tatccctcaa acatcttgaa tgcctgtgcg gcgcccggca gataatcctt
     5821 ctccagattc gggaatttat ctttatacgc ggggtaaatc ttctcgtaca aacccatttc
     5881 aacaccggaa gccattgcgt cgtatccggt gagcaccatg gtggtgtcaa ggtttccggc
     5941 ggcttgctgg gccttgatct tcgccggtag ctccggagcg gttgccttaa tatactcgat
     6001 atctgaaacc caatccggat gtgcggcttt aaatgcttca atcgcaggtt tggtcaactg
     6061 aagatttcca gccacatcga tgatgctgag cttcaccggt ttggagacca aactttcggc
     6121 tccaaggccg gccgtaagga cgctgagaat catggtaaca gccaaaacaa aaaccagcaa
     6181 tctgaatctt ttcaacgcaa ctacctccct tagtttttta ggagcatatg tcctaatttc
     6241 ttaacaataa tttatccgaa tattaacaga ttccctctaa agaatattgg caaatatagc
     6301 acattcttgt cacaaaatat ttatgataaa ccgcatcatg ttatacggtt taatgcggtt
     6361 tatttacctt atactcggta ggcgtcaccc cgctaatttt cttaaaaacc ctcgcaaaat
     6421 agttgggatc tttataaccc acctgataac aaacctcctt gaccgaactc aggggattgg
     6481 ccagaaactc cttggatttc ctgatacgca acccggtcag ataatcaata aagttctcgc
     6541 cagtctcttt ttt
//
LOCUS       DASVMB010000253         7792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_473649,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000253 DASVMB010000000
VERSION     DASVMB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473649"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..2356
                     /locus_tag="VF531_07435"
     CDS             179..2356
                     /locus_tag="VF531_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016402333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7713841.1"
                     /translation="MHDYQNSTLSTERRVNDLLSRMSIEEKIGQMMQVSYNQVSPAQA
                     EDWVTKGYAGSFLHVLGDNARHLQELSESTPLGIPILFGIDAIHGHALNDRATVFPTQ
                     LAVSCSWNGTLIEEMGRVTAREVVADGLHWTFSPVLCIGRDLRWGRIDETFGEDPYLI
                     GMLAAALIKGYQGKSLSDRDSILACAKHYLAYGESNGGRDSYDTEVSFRKVRETFLPP
                     FQMAVEAGCATVMAGYQSLDGTPVSASQKLLREILKDELGFTGFVVTDWNNTGSFIHY
                     QRVAADMETAVQKAAAAGNDMIMNTNAFYDAAVELVKKGVITEELIDEAVKRVLSVKF
                     SMGLFDEKRRVQAAVQTEIFNCPEHLDVNLRITRESMVLLKNKNHTLPLGNNLKRIAV
                     IGPNADDIQAQFGDWTLFSHPDPNPDALPLAPYHTMLAGIKELAGRRGIEVSYHKGCD
                     VMDPGLQDIAAATALAAQADAVVAVIGDCLDQNGECKDRADLSLSGAQSLLLERLKDS
                     GKPLIVILVNGKPLAIPWVEEYADAILETFNSGALGGKAAAEILFGEVNPSGKLTISF
                     PYHSGQLPVYYNQLPGWHGNGKYMDMPSEPLYSFGYGLSYTSFKYSNLKLSKTECSVG
                     DTVQVQVQVTNTGLVDGMETVQLYVRDVTSSVVTPVKQLRGFEKVPIGKGETRTVVLP
                     LRISDLYIVTPDEKYLVEPGEFTIMVGSSSRDCDLLKASLWVR"
     gene            2643..3641
                     /locus_tag="VF531_07440"
     CDS             2643..3641
                     /locus_tag="VF531_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX7713842.1"
                     /translation="MPLQTVLPRWRGFNLLNFYTPRSDRQFNEEYFRWIAEWGFNFIR
                     VPMDYHLWTPGNSMAKLDEAVLERLDRAVVLGERYNLHISLNLHRAPGYCINPGVREP
                     FNLWKDEAALEAFSMQWQMFSRRYSGIAPDRLSFDLVNEPPQPSPEVMTRGDYERVVR
                     RTVAAIREISPERLIMIEGVAVGNEPSPEMADLQVIQSCRAYIPMGISHYQARWAGGE
                     RYPKPVWPGAIHEDGRLWDRKLLEEHYAKWGELARKGVRVHCGEGGCYNQTPHHVFLA
                     WFRDVLEILTAHNVGYSLWEFSGNFGILDSGREDVAYQDWHGHQLDRELLELLQKF"
     gene            3760..4098
                     /locus_tag="VF531_07445"
     CDS             3760..4098
                     /locus_tag="VF531_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7713843.1"
                     /translation="MNRVNQCQLVANESDLNEALKLNEQMLTLVFATWCPFCQRFLPV
                     FEKYARGREDFLLIQDDQEIVADRYEVEVVPTALYFEKGILEKRLDGELGVGLSERQL
                     RDFIKECGLT"
     gene            complement(4110..4247)
                     /locus_tag="VF531_07450"
     CDS             complement(4110..4247)
                     /locus_tag="VF531_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713844.1"
                     /translation="MVYLAILGAILVLIGNNTKNRISKPLLISGLILTPIGLVLMWIY
                     R"
     gene            complement(4394..4756)
                     /locus_tag="VF531_07455"
     CDS             complement(4394..4756)
                     /locus_tag="VF531_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX7713845.1"
                     /translation="MGIMQTVLNALGLNEQNNEEFGSFDDDRISWNPDPARPGETVEI
                     EYRGLLPNSGADQVYLHYGFDSWNRQVRTIPMEKKEDGAFRAAILAEGEHEINFCFKD
                     AGENWDNNNGNNWTLHLV"
     gene            5472..>7792
                     /gene="secA2"
                     /locus_tag="VF531_07460"
     CDS             5472..>7792
                     /gene="secA2"
                     /locus_tag="VF531_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEX7713846.1"
                     /translation="MIKNLANLQKRVDFNRAPVEFDLRPYQRLAEEIQRIDWSDWSSE
                     NLVRFSLQLRKQAQDGTPQDELLVPAFALVCEVSHRLLGQRPFREQLLAGIALHQGKI
                     VEMQTGEGKTLAAVMPAFLNGLTGKGIHILTFNDYLAERDARWMGPIYEFLGLTVAYV
                     QGGMSANERKKAYAADITYLTAKEAGFDHLRAFLVYDPSERIQRPFHCAIIDEADSIL
                     IDEGRIPMVIAGNSSPGDNTELNRCAQVVRKLRPGIDYTLDEYRRNVYLTEQGISRAE
                     QELGCGNLYIPENLILLTRLNNALHAQALLQRDLDYIVREGRVELVDEFTGRVMLNRH
                     WPAGLQEAIEAKEGLTTQPGGRILGTMTLQNFLKNYPRLAGMTGTAQAGADLFKEFYN
                     LDVTVIPPHRPGIRRDEPDLIFTDREAKMAALLQEIARIHSSGRPILVGTSSIEESEE
                     LATKLAETGITCQLLNAKNDRWEAELIAEAGARGGVTISTNMAGRGTDIRLGGRDGRD
                     YNAVVALGGLYVIGTNHFESTRIDNQLRGRAGRQGDPGSSRFFISLEDELVERYGIRQ
                     SLPEKYRQCHQETPLTDPAVLRLVSGSQRIIEGQNHEIHRQLWKYSWVAERQRRRMME
                     RREKILLGQDGSGLFQNGLPERYHALAEHIPVPVLSRVEKQVSLSVIDRLWADYLEEV
                     ESIREGVPLVQLAGKVPLDEFNKSVWEAYLDLECRIDAEIIAALGRVNITAEGIDLDR
                     EGLRGPSATWTYLVNDTPFGGRLHTILAPLLKKV"
BASE COUNT         1947 a         1772 c         2074 g         1999 t
ORIGIN      
        1 ctccgggcgg aagattatcc ggtgcacatt caatggttag aaatattgcc atccgggttt
       61 taagaagaaa taaatcccag aacggagagg ataatcgcgt ctttaatcga ataagtgtat
      121 acccgagaat ccggtaaata attattttga aactcaaaaa aggaagccgt ttataaccat
      181 gcatgattat caaaactcca cactctccac cgagcgaagg gtcaacgatc tgctttccag
      241 gatgtcgatt gaagagaaaa tcgggcagat gatgcaagta agctataacc aggtaagtcc
      301 cgcccaggcc gaggattggg tcaccaaagg atatgccggt tctttcctgc atgtgctggg
      361 ggataacgca agacacctgc aggagttgag tgaatcaacc cctttaggca tcccgatctt
      421 atttgggatt gacgccatcc acggacatgc cctcaacgat cgcgccactg tattccctac
      481 ccagcttgcc gtctcctgca gttggaacgg gacgttaatt gaagagatgg gacgtgtgac
      541 tgcacgtgaa gtagtcgcgg acggtctgca ctggactttt tccccggttt tatgcatcgg
      601 cagggatttg aggtggggga ggattgatga aaccttcggg gaggatcctt atttaatcgg
      661 gatgcttgct gcggccctca tcaaaggtta tcaaggaaaa tccctatcgg accgggatag
      721 cattctcgca tgcgccaagc attatttggc ctacggtgag agtaacggcg gcagggattc
      781 ttatgatacg gaggtttcct ttagaaaagt cagggagacg ttcctgccgc cgtttcaaat
      841 ggcggtggaa gccggatgcg caactgtaat ggcgggctat cagtccctgg acgggacccc
      901 cgtttcggca agccaaaagc ttttaaggga aatattaaag gatgagctcg gattcacggg
      961 ctttgtggta accgactgga ataataccgg aagtttcatc cattatcagc gggttgccgc
     1021 ggatatggaa accgcagtcc aaaaggccgc cgcggccggc aacgatatga ttatgaacac
     1081 caacgccttt tacgacgccg cggttgagct cgtcaaaaaa ggcgtgatta cggaagagtt
     1141 aattgacgaa gcagtgaagc gggtgctatc cgtcaagttt tccatgggcc tttttgatga
     1201 gaaacggcgg gttcaagcgg ctgttcaaac ggaaatcttt aactgtcccg aacacttgga
     1261 tgtgaatttg cgaattaccc gtgagtccat ggtgttgctg aagaataaaa atcataccct
     1321 tcccctggga aacaacctga agcggattgc ggtgatcgga cccaatgccg acgatattca
     1381 agcccagttc ggggactgga ccttattttc ccatcccgat cccaacccgg atgcgttgcc
     1441 gcttgcgccc tatcatacca tgctggccgg gattaaggaa ctcgccggga ggaggggaat
     1501 cgaggtctct tatcacaagg gttgtgacgt aatggatccc gggcttcagg atattgcggc
     1561 cgccaccgct ctcgcggccc aagcagatgc ggtcgtcgcg gtaattggag actgccttga
     1621 tcaaaatggc gaatgcaagg accgggcgga tttaagccta tcgggagcgc agtctttatt
     1681 gctggaacgg ctgaaggatt cggggaaacc actcatcgtc attttggtca atgggaaacc
     1741 gctagctata ccatgggtag aggaatacgc cgatgcgatc ctggagacct ttaattctgg
     1801 ggcattggga ggcaaggccg ccgccgaaat tctgtttggc gaggtcaatc ccagcggcaa
     1861 gctgacgatc tccttcccat atcattcagg tcaactcccg gtttattaca accagcttcc
     1921 gggctggcac ggcaatggta agtatatgga catgccgtcg gaacctcttt acagtttcgg
     1981 atacggttta tcctatacaa gtttcaaata ctccaacttg aagctttcca agaccgaatg
     2041 ttccgtcggg gatacggttc aggttcaggt tcaggtgacg aataccgggt tggttgatgg
     2101 aatggaaacg gttcagctct atgtgcggga tgtcacgagt tcggtggtga ccccggtcaa
     2161 gcagttgagg ggttttgaaa aggtcccgat tgggaagggt gagacccgga ccgtggtttt
     2221 gccgttacga atctccgacc tgtatatcgt aaccccggat gagaagtatc tggttgaacc
     2281 cggcgaattt acgatcatgg tcggctcaag ctctagggat tgtgatcttt taaaagcatc
     2341 gctatgggtc cggtgagcta actcccaccc tgctctcata ctcattttcg tgtctttggt
     2401 gtgtttggtt gtgaatgaaa ttgcacaccg atagaaactt gtttaaccac gaaaaacacc
     2461 aaatacacga aatctctttt caggcgatat tctttaaacc cgaaaagagg atgattctcc
     2521 ataggacatc cgtaaaaacg gagtcctttt ttagttgaag aaggtttcac gatgtcatcg
     2581 ccgaatttta tttatattcg taaatcattt aggcctttac ccgtatagaa agggttgatt
     2641 tcatgccatt acagactgtc ttgccccgat ggcgggggtt taacctactc aatttttaca
     2701 cgccccggag tgatcggcaa tttaatgaag agtatttccg gtggattgcc gagtggggtt
     2761 ttaatttcat ccgggtgccg atggattacc atttatggac tccgggtaat tccatggcta
     2821 aattggatga ggcggtgcta gaaaggttgg atcgcgcagt tgttcttggg gaacggtata
     2881 acctccacat ctccctcaat ctgcaccggg cgccgggtta ttgtattaat ccgggagtca
     2941 gggaaccttt taatctttgg aaggatgagg cggcgcttga agctttttct atgcagtggc
     3001 agatgttcag caggcgctat tccgggatcg ccccggatcg cctaagtttt gatttggtga
     3061 atgagcctcc gcaaccctca ccggaggtga tgacccgggg cgactacgag cgggtggtac
     3121 gaaggactgt tgccgccatc cgtgagatca gcccggagcg gttgatcatg attgaagggg
     3181 tggcggtagg gaatgaaccc agtcccgaaa tggcggattt gcaggtcatc cagagctgca
     3241 gggcttatat tccgatgggt atcagccatt atcaggcccg ttgggcgggg ggtgaaaggt
     3301 atcccaaacc ggtctggccg ggtgcgattc atgaggatgg caggttgtgg gatcgtaagc
     3361 tcttggaaga gcattacgcg aagtggggcg agcttgcacg aaaaggcgta agagttcatt
     3421 gcggtgaagg cggctgttat aaccagaccc ctcatcatgt atttctcgcc tggtttaggg
     3481 atgttcttga gatccttacc gctcataatg tcggttattc gctttgggag ttttccggca
     3541 atttcgggat tttggattca ggccgagagg atgtggcata tcaggattgg cacggacatc
     3601 aattggaccg ggagttgctt gagttattac aaaagttctg aaagtaatgg agcccttccg
     3661 gaatccatag aggggcttta ttattctatt ttcaaaaccg tcaaatctcc ttgaatcaaa
     3721 aatggaattg gaacataata aagtgagggg ggatttataa tgaaccgagt caaccagtgc
     3781 caattagtcg cgaatgaatc ggatctaaat gaggcgctca aattgaatga gcagatgctg
     3841 accttggtct ttgccacatg gtgcccgttt tgccaaaggt ttttaccggt atttgaaaag
     3901 tatgcccggg gtcgggaaga cttcctgcta attcaagacg atcaggagat cgtggcagac
     3961 cggtacgagg tagaggtagt tcccaccgca ctctactttg agaagggaat cctggaaaag
     4021 cggcttgacg gggagctggg tgtgggatta agcgagcggc agttgcgtga tttcattaaa
     4081 gaatgcgggt taacgtaata caatccggtt tagcggtaga tccacattaa aaccagtccg
     4141 atgggggtca agatcaaccc ggaaatcagc aggggtttac ttatccggtt ttttgtgtta
     4201 ttcccgatta aaaccaggat tgctccgagg atcgccagat aaaccatact atccacctcg
     4261 catcaaagtt tgacgtttga agagaacgaa tttattatac catatttccg gtaaagcccg
     4321 ggtaaaattt aaaggggctg gtgaaagccc ctttgtagac tggcgaagga tatggatgaa
     4381 accggaaaaa tatctaaacc aggtgtaaag tccagttatt gccgttattg ttatcccaat
     4441 tctcaccggc atctttaaaa cagaagttga tctcatgttc tccctcagcc aggatggccg
     4501 cacggaaagc cccatcttcc tttttctcca tcggaatcgt gcgcacctgc cggttccaac
     4561 tgtcaaaacc gtaatgcaag taaacttgat ccgccccgga gttgggcaaa aggccccgat
     4621 attcaatctc tacggtctct ccgggtctgg ccggatccgg attccaggaa atacggtcgt
     4681 catcaaaact gccgaactcc tcattgttct gctcattcaa tcccagggca tttaaaaccg
     4741 tttgcataat tcccataagg ttcaccccct ccggcttttt atgttagtct ccccgtattg
     4801 cctttggtat agcccgtaag ttaaacccgg tttcgacaaa ccttcccaac taaaaaaggt
     4861 tctttctcaa acggaccgaa ttatatacgg aaggtacgtt ggcgagcgat ggttgttttt
     4921 caaattatcg ggcaaactta agggaaatct cttttcttgg aacttattgg ggccaaccgt
     4981 cgttttaacc ataatagatg aaaggaggaa gcattttgag gcggcctata aagcgttatc
     5041 tattcatcgt tctatgcgta gctttattac tctttgcagt gcttttgcct caaagctgtt
     5101 tcgttctgct ctcctttgcg ggactcttct ttttctggat tttcctcttt atttaccctg
     5161 aaaccacaaa attaccggtt cggattttcc ccggttccaa tctatcggca cgtgcacctc
     5221 cctctctctt ttaatccatt ttttcacttt tcaaataaat agcaaaaaac ctcaagcgtc
     5281 tttgggatgc tttatttggc gtgctatttt ttgctttgtt ccttaatggc tacaatcaaa
     5341 agaatatctc ccgccaaggc gcatttattc attctctgcg cctctgcggg agaataataa
     5401 aggacatatt gaatcaacta tttttttgaa aacaagcgaa aaatttattt atctattgat
     5461 ggaggtacat aatgataaaa aatctggcca acctgcaaaa acgggttgat tttaaccgtg
     5521 caccggttga gttcgatctt cggccgtatc agagattggc agaggagatt caaagaattg
     5581 attggtccga ttggtcatcc gaaaacttgg ttcgtttctc cctgcaactt cgtaagcaag
     5641 cccaggatgg taccccacag gatgagctgt tggttcctgc tttcgccttg gtttgtgagg
     5701 tttcccatag gcttttgggt caaaggcctt ttcgggagca gctattagca ggcatcgcgt
     5761 tgcatcaagg gaagatcgtg gaaatgcaga ccggagaggg taagaccctg gcggcggtga
     5821 tgccggcttt tttaaacggt ttaactggga aagggattca tatcctgacc ttcaacgatt
     5881 atctggccga aagggatgcc cgctggatgg ggcctatcta tgaatttctc ggtttaaccg
     5941 tagcttacgt ccaaggggga atgagcgcga atgagcggaa gaaggcatat gccgcagata
     6001 tcacctacct cacagccaag gaagccggtt ttgatcacct gcgagcattt ttggtctacg
     6061 acccatccga acgcatacaa cggccttttc attgcgccat cattgacgag gcggattcaa
     6121 tcctcatcga tgaaggtcgg atcccgatgg ttatcgccgg aaacagttca cccggggata
     6181 atacggaatt aaaccgctgc gctcaagtgg ttcgaaagtt acggcccgga atagattaca
     6241 cccttgatga gtatagacgg aacgtctatc taaccgagca gggaatatcc cgggccgaac
     6301 aggaactagg ctgcggtaat ctttatattc cggagaacct gatcttatta accaggctta
     6361 acaatgccct tcatgcgcaa gccctcttgc aacgtgatct ggactatatt gtccgcgaag
     6421 gccgtgtgga gttggtcgat gagttcacag gccgggtaat gcttaaccgt cattggcctg
     6481 ccggattaca ggaagcgatt gaagccaaag aaggtttgac cactcagccg ggggggcgga
     6541 ttttgggtac gatgacgctg caaaattttt taaaaaacta tcccaggtta gcgggaatga
     6601 caggaacggc acaggccggg gcggacttat tcaaggagtt ttataatttg gatgtgactg
     6661 ttattccccc acatcgccct ggcattcgtc gggatgaacc ggatctgatc tttacggatc
     6721 gtgaagcgaa aatggcggcg ctacttcaag agattgcccg gatccactcc tcggggcgcc
     6781 caatcctggt agggaccagc agtattgagg agtcggaaga gctggccaca aaacttgccg
     6841 agacgggaat cacctgtcaa ctattgaatg ctaaaaatga tcgttgggag gcggagctta
     6901 tcgccgaagc cggcgcccgg ggcggggtta ctatctcaac caatatggcg ggacgaggaa
     6961 cggatatccg actcggcggg cgcgatggcc gtgattataa tgctgtagtc gccttgggcg
     7021 gcctttatgt catcggaacg aatcatttcg aaagtacccg gattgataat caactgcgcg
     7081 gccgcgcagg tcgccaggga gatcccggat caagccgttt tttcattagt ttggaggacg
     7141 aattagtaga acgttatggg atccgtcaat cacttcccga aaaatatcgc cagtgccatc
     7201 aggaaacccc attaaccgat ccggccgtac tgcggctggt ttcgggatca caacggataa
     7261 tcgaaggtca aaatcatgag attcaccgcc aattatggaa atactcctgg gttgccgagc
     7321 ggcagcgtcg ccggatgatg gaacgcaggg aaaagatact gctgggacag gatggttcgg
     7381 gactttttca aaacggcctc ccggaacggt atcatgcctt ggcggaacat attccggtac
     7441 cggttttaag ccgcgtagaa aaacaggtta gtctatctgt gattgaccgt ctttgggccg
     7501 attacttgga ggaagtcgag tcgatccgcg agggggtgcc gttggtgcaa ctagccggaa
     7561 aagtccctct ggatgaattc aataaatcgg tctgggaagc ttatctggat ttggaatgcc
     7621 gaatcgatgc ggagatcatt gccgctcttg gccgggtaaa cattacggca gaagggattg
     7681 acctggaccg ggaaggcttg cggggaccgt cggccacctg gacatacttg gtgaacgata
     7741 ctccatttgg gggaagactt cacacaattt tagccccact tttgaaaaag gt
//
LOCUS       DASVMB010000254         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_31901,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000254 DASVMB010000000
VERSION     DASVMB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31901"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VF531_07465"
     CDS             complement(<1..500)
                     /locus_tag="VF531_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7713847.1"
                     /translation="MAGKQIVFDEEARQALQRGVDKLANAVKITLGPKGRNVVLEKKY
                     GSPTITNDGVTIAKEIELPDPFENMGAQLAKEVATKTNDIAGDGTTTATLLAQAMVRE
                     GLKNVAAGANPMILKRGIEKAVQVVVEEIKKVSQPVDRKEAITQVATISAADEEIGTL
                     IAEAME"
     gene            complement(529..819)
                     /gene="groES"
                     /locus_tag="VF531_07470"
     CDS             complement(529..819)
                     /gene="groES"
                     /locus_tag="VF531_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7713848.1"
                     /translation="MNIRPLGERIVLKVLESEEKTKSGIVLPDTAKEKPQMGEVMAVG
                     NGRILDNGQKVALEVKPGDKVLFAKYAGTEVKLDGEEYMVLKESDVLAIVNQ"
     gene            1381..1554
                     /locus_tag="VF531_07475"
     CDS             1381..1554
                     /locus_tag="VF531_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713849.1"
                     /translation="MNSKPSRARANPYLLFLILILLLLSFGRYGFMLFLILMLLLLSF
                     GGYGATIPNHDSS"
     gene            complement(1529..2254)
                     /locus_tag="VF531_07480"
     CDS             complement(1529..2254)
                     /locus_tag="VF531_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713850.1"
                     /translation="MERDRTVSATIRLIIADDMLPIREYLSLVLSHEPDMQIIAAVGT
                     GAEAVEKAFSATGSDHPDVILMDLEMETPRAGVEAIHTLARKAPEIRCVVLTHFSDDD
                     TVFAAFEAGATDYVLKDASAAEILEAVRAAARDMSPLRPQIARMIRSEFRAMRSERAL
                     LTATLNIVYRLTPTELNLLRLLAEGKSRSEIAQLRHVEASTIRTHVGNILKKFGESSV
                     GDVVDRLRRLGIFEIFTTNHDSE"
     gene            complement(2364..>2775)
                     /locus_tag="VF531_07485"
     CDS             complement(2364..>2775)
                     /locus_tag="VF531_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713851.1"
                     /translation="FSRYEVRAKTYEEGKCIDPDPLVVQKYVKKNPTVHGFEAFAGTL
                     NQDYLATYPIMPGWKAPTQNPCTVFRQILLRGGNLEAELERLNQYWNKKLDEYYRQHP
                     QVKRGLNIYPHFNRITGNMGAPLVKPGFSIPRGN"
BASE COUNT          660 a          768 c          580 g          767 t
ORIGIN      
        1 ttctccatag cctcggcgat cagtgtcccg atctcttcgt ccgccgcgga gatggtagcc
       61 acttgagtaa tagcttcttt cctatcaacc ggctggctga ctttcttgat ctcctcaacc
      121 acgacttgaa cggctttttc aataccccgt ttcaaaatca tcgggtttgc gccggcagcc
      181 acattcttca agccttcgcg aaccatggcc tgggcaagca aagtggcggt agtggtgccg
      241 tctccggcga tgtcattggt tttggtcgcg acttctttcg caagttgcgc tcccatgttc
      301 tcaaatggat ccgggagttc gatctctttg gcgatggtca cgccgtcatt ggtaatggtc
      361 ggagaaccgt atttcttctc cagtactacg ttacggcctt tgggacccag ggtaatttta
      421 acggcatttg ccaatttatc gacgccgcgt tgtaaagcct ggcgggcctc ttcatcaaat
      481 acaatctgtt ttcctgccat aatctcacct tctttaagtt attggttatt actgattcac
      541 gatggctaaa acatcgcttt cctttaaaac catgtactct tcgccgtcca gcttgacctc
      601 ggtcccggca tatttcgcga acaacacctt gtcgcccggt ttgacttcca aggctacctt
      661 ttggccatta tccaaaatac ggccgttacc taccgccata acctcaccca tctgcggttt
      721 ctctttggcg gtatcgggaa gtacaatccc gctctttgtc ttttcttcgc tttccaaaac
      781 ttttaaaacg attctctctc ccaatggtct gatattcata atatccctcc ttactgcgtt
      841 tattgttagc actcactgaa agcgagtgct gatttctaaa aacaatttta acatctgaaa
      901 taggttttgc aagagaacct cttcattatt attcccattt tggattaaat aaacgtaacc
      961 taatgcgttg aaatggaaat atctcgataa ggtttaaaat tacaccggca cattccggtt
     1021 tttagggaca tatcaaaaaa acttccgtga ctttacaatg tcaaaagtgg ataaagcctg
     1081 taacaaacgg ggtcccccga tataacccga tttccaaacc attcggtttt ttaccaaatt
     1141 gtaaaccaaa gaacacaatc gtatatcccc gttcataagg gccattcctc ctcaggacag
     1201 gagcttgctt gaaaatactt ccagcccggt acgaacggaa aaaagcgcca atagtctata
     1261 gtattccccc ggtggcgaaa ataactttca cgaaacggtt gattccgact tccatccatc
     1321 aacaattcgt atcatcaaac cactggagaa cagacccatc gggccgagag gatggctacc
     1381 atgaactcta aaccttcgcg cgctcgcgcc aatccttacc tgctgtttct aattctcatc
     1441 ttgttgttgt taagcttcgg cagatacggc ttcatgcttt tcctgattct catgctgttg
     1501 ttgttaagct tcggcggata cggtgccact attccgaatc atgattcgtc gtgaagatct
     1561 caaatattcc caagcggcgc aatcgatcca caacatctcc gaccgagctc tcaccgaatt
     1621 tttttaaaat gtttcccaca tgagtccgga tcgttgaagc ctccacgtgc cgcagctgtg
     1681 caatctcgct acggctcttg ccttcggcaa gcagccgtag cagattcaac tcagtaggag
     1741 tcaagcgata tacaatattc agcgttgcag taagtaacgc ccgctccgaa cgcattgccc
     1801 ggaattcgct ccggatcatc ctggcaatct gcggccgaag cggcgacata tcgcgggcgg
     1861 cagcccgaac cgcctccaga atctcggcgg cggaagcgtc tttgagtaca taatcggtgg
     1921 caccggcctc aaaggcggca aatacggtat catcgtcact gaaatgagtt aagaccacac
     1981 agcggatctc cggggcctta cgggctaagg tatgaatggc ctcaaccccg gctcgcggag
     2041 tctccatctc cagatccatc aatatgacat ccggatgatc cgaacccgtc gcggagaacg
     2101 ccttttccac cgcttcggct ccggtaccaa ccgccgcaat aatctgcata tccggttcat
     2161 gactcaagac caaactgaga tactcccgaa tcggcaacat atcatccgcg ataatcagac
     2221 gtatcgtggc cgataccgtt cgatcccgtt ccatgttttt tcctcctgtc aaaaagtcgt
     2281 accgtctaaa gtccgaagtc ataagcctaa cagtgttgcc tgttgcctgt tgccgcgcgc
     2341 ccattgggcg cgatgcctct tgcctaattc cccctgggta tactaaaccc gggtttaacc
     2401 aatggtgccc ccatatttcc ggtgatccgg ttaaaatggg gataaatatt caacccccgc
     2461 ttaacttggg gatgttgccg gtagtattca tccagtttct tattccagta ctggttcaac
     2521 cgctccagtt ccgcttccaa atttcctccc cgcaataata tctggcgaaa aaccgtgcat
     2581 ggattttgag tcggagcttt ccatcccggc attatcgggt aggttgccag ataatcctgg
     2641 tttaaagtgc cggcaaaagc ctcgaaccca tgaacggtag gattcttttt cacatatttc
     2701 tgtaccacca atggatcggg gtcgatgcat tttccttctt catatgtttt tgcccgtact
     2761 tcgtaacggc tgaac
//
LOCUS       DASVMB010000255         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_547318,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000255 DASVMB010000000
VERSION     DASVMB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547318"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF531_07490"
     CDS             <1..199
                     /locus_tag="VF531_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713852.1"
                     /translation="DSQGNKFQKRFNKLQGAGSYLIVSFVLLAGLSINYFSGWWQADA
                     IAAFTIVIFIFKKGIETALKK"
     gene            complement(286..1095)
                     /locus_tag="VF531_07495"
     CDS             complement(286..1095)
                     /locus_tag="VF531_07495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713853.1"
                     /translation="MRRLPPSKFDLLNFSVPDEPDNCSTNFLLHRSTSTILVDNPLDP
                     KMLVVSYPTDSPPPMYFIYGKAERPLSIQRLLQELQTPTDLVVPNSMLNMVSHYWPVR
                     FSVPVMFLAAPKGHWQPQTPTNYPVRLLTENDTPQLARNFSDDPWLWEFFFTPDQLIR
                     EGMAVGAFVNGHLACVATTLAFTERYVELGVATGPEYRGLGLALECCRALSRIQYEQF
                     GRLPCWRTQVHNVASWKVALKLGLEELDRSEKYLFLSNYQHVGAYAAMVAP"
     gene            1686..2735
                     /locus_tag="VF531_07500"
     CDS             1686..2735
                     /locus_tag="VF531_07500"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7713854.1"
                     /translation="METRILTITGTRRNGLSEAARILQNGGLVAFPTETVYGLGAVCT
                     DDDAMRRVFAVKGRPGDNPLILHIWRVEQLLELAAEICPKAFRLIEAFWPGPLTIIFP
                     KQPTVSAVATAGLDTVAVRMPSHPVTRELLRLTDIPVAAPSANLSGKPSPTKGSHVVD
                     DLAGKIEMIIDAGPCNAGIESTVLTLAAEHPLILRPGSVTREMLQAVLNETVEVSNPA
                     VPSERPQAPGMKYRHYAPQAPAFLVEGIDNRKVVAHINQLLAAAKGEKPGLRPVVLGT
                     TENLKDYNTEFVLDLGSRHDPAMVAARIYDLLRFCDQLAADIIYIEGISAQGVGLAVA
                     NRLYKAAGGNVIRVS"
     gene            2725..3501
                     /locus_tag="VF531_07505"
     CDS             2725..3501
                     /locus_tag="VF531_07505"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7713855.1"
                     /translation="MFRKAGLLLLLLLVMTWGCIIQAQANPMPGANNYLPQVGEVLQY
                     NVYVQNIFHGAVQTVKILSRDELNNRPVLNVQLSMETIGLVNRVAKYQETEALTLDLD
                     GLYPVALHREVQQSDGITIEEVRFDYAKAVATREVTHPDGNIESSLIKLPGYIQDAAS
                     LQFFLRKYQFNVGANQLYFYSNGTIASVNFIVTPGKTAIKLESGLYNRYYQIDDPIDR
                     ITVLVAQDAQRLPFLIRKYTSFGKVEAKLIKVSNTVITSQ"
     gene            complement(3550..>3772)
                     /locus_tag="VF531_07510"
     CDS             complement(3550..>3772)
                     /locus_tag="VF531_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713856.1"
                     /translation="EGMCSSSNWPFADAYGYMILKCPSCPNRRIFSAAALQERLVWRC
                     PECGNQLNLNLERVSVRYPAGAFDNERYQ"
BASE COUNT          981 a          889 c          938 g          964 t
ORIGIN      
        1 cgatagccag ggtaataagt ttcagaagcg cttcaacaag ttacaggggg ccggaagtta
       61 tctgatcgtc tcctttgtac tgctcgccgg tctctccatc aactattttt ccggttggtg
      121 gcaggcggat gccatcgccg ccttcacgat tgtaattttc atctttaaaa aagggatcga
      181 gacagccctg aagaagtgat gaatctttgg cgcgcgcgga aatattgaaa gccgtaaaaa
      241 aacggactac gcaagaacgt agtccgtttt gatattttat ccttcttaag gagccaccat
      301 tgcagcatag gctcccacat gttggtagtt ggagaggaaa aggtatttct cgctcctatc
      361 taactcctca aggccgagtt tcagggcgac tttccaggaa gccacattat gcacctgggt
      421 tcgccaacaa ggcaaccgtc cgaactgctc gtattgtatt cggctcaatg cccggcagca
      481 ttccagcgcc agtccgagcc cgcgatattc cggtccggtc gctaccccaa gttccacata
      541 acgttcggtg aaggcaaggg tcgtcgccac acaggccaga tggccgttaa cgaaggctcc
      601 caccgccatg ccttcgcgga tcaattgatc cggggtgaag aagaactccc agagccaagg
      661 gtcatcactg aaattccgag ctaattgcgg ggtatcgttc tccgttaaga gtcgtaccgg
      721 gtaattggtt ggagtttgag gttgccaatg acctttcggg gcggccaaaa acatcacggg
      781 tacagagaaa cgcactggcc aataatggct gaccatgttc aacatggagt tgggaacgac
      841 caaatcggtt ggggtttgaa gttcctgtaa tagacgttga atgcttaggg gtctttcggc
      901 cttaccgtaa atgaagtaca ttggcggggg agaatcagtc ggatacgaaa ccacgagcat
      961 tttagggtct agagggttat ccaccaagat cgtactcgta ctacgatgta ataaaaagtt
     1021 ggtcgaacaa ttatcaggtt catcgggaac agagaaattc agtaaatcga acttactagg
     1081 aggtagcctc ctcatattgt tcatctccta agtgatttga acattcacaa cggattgatg
     1141 tggacgaggc tacttcgcta cgcataaggt actaaaatga tttgttcccc cctttagatt
     1201 caaacatctt acttatgata tcggcaaaag cctgattgag catcagagga ttttatgaac
     1261 gatctctcag atatgtttca ctcatcatga attttatgac tcaaatacga cttgaaatgt
     1321 ttaaaactgg gacttgttat atttaagcat acacctgatt tttgatgctg taaatagtcg
     1381 ttttttatcc aaccggcatt aaattttacc tgcaatcttc gcctcgtgcc gccggaggtt
     1441 atatactgaa cgaggtgcag atagatgacc agtccttttt aactaaaatt taccggtctc
     1501 atctcaagct ggttatagcg gtgcaaaaaa gacagtttgg agagggaaag tttattcgat
     1561 atcgtgcagg aaaaaattat ctggggtaaa atagatctat attgaaaatt gtacgaaaaa
     1621 ctccgttttt taactggcac gccggtgcgt gaaaaattag gattcactat ggatgcgata
     1681 tctcaatgga aaccaggatt ttgactataa ccggtacccg ccggaacgga ctgagtgaag
     1741 cggcccggat tttacaaaac ggcggtctgg tggcttttcc gactgaaact gtatacggcc
     1801 tgggtgcggt ttgtaccgat gacgacgcga tgcggcgcgt ttttgcagta aaaggacgac
     1861 cgggggataa tcccctaatc ctgcatatct ggcgggtgga acagttactg gaacttgctg
     1921 cggagatctg tcccaaggcg tttcggttga ttgaagcttt ttggccgggc ccgctaacca
     1981 tcatttttcc caaacagccg acagtctccg cagttgctac ggccggtttg gatacggtgg
     2041 cggtacgcat gccatcccat ccggtaaccc gtgagttact gcgccttacc gatattcccg
     2101 ttgccgctcc cagcgccaat ctttccggta aacccagtcc taccaaaggc tcccatgtag
     2161 tggacgatct ggcgggaaag atcgagatga tcatcgatgc cgggccctgc aatgcgggga
     2221 ttgagtcgac cgtattgaca ctggccgccg agcatccctt gatcttgcgt cccggttccg
     2281 tcacccggga gatgctccag gcggtactga atgaaacggt agaggtatcc aacccggcag
     2341 tgccaagtga acgaccgcaa gccccgggta tgaagtatcg ccattacgcc ccccaggctc
     2401 cggccttttt agtcgagggc atcgacaacc gcaaagttgt ggcacatatc aatcaactat
     2461 tggccgccgc caagggtgaa aaaccgggtc ttcggccggt ggtgctcggg accaccgaaa
     2521 atctcaagga ttacaatacc gagtttgtat tggatctggg atcgcggcac gatcccgcca
     2581 tggttgcggc ccgtatttac gatttattaa gattttgtga tcaactggct gccgatatta
     2641 tctatatcga gggaatctct gctcagggag tcggattggc agtagccaac cggctataca
     2701 aggctgcagg ggggaatgtg atccgtgttt cgtaaagccg gattgctgct gctcttattg
     2761 ttggtaatga cttggggttg tattatacag gcacaagcaa atcccatgcc cggcgcaaat
     2821 aactatcttc cccaagtcgg agaagtattg caatacaatg tttatgtgca aaatatcttt
     2881 catggagccg ttcaaaccgt aaaaatcttg tcccgggatg agctgaataa ccgtccggta
     2941 ttaaatgtcc agctatctat ggaaacgata ggattggtca accgggtggc gaaataccag
     3001 gagacagagg cgctgactct agatttggat ggattatatc ccgtggcttt acaccgcgag
     3061 gttcaacagt ccgatgggat caccatcgag gaagtccgtt tcgattacgc caaggcggtt
     3121 gccacccggg aggttaccca ccccgacgga aacattgaat ccagtttgat aaaactcccc
     3181 ggttacattc aggatgcagc ctcactccag ttttttctaa gaaaatacca gtttaatgtg
     3241 ggcgccaacc aattatactt ttattccaat ggaacgattg cctcagttaa ttttatagtt
     3301 accccgggaa aaacggcaat taaattagag tccggtctct ataaccggta ctatcaaatc
     3361 gatgatccta ttgatcgaat tactgtgctg gtagcccaag atgctcaacg attgccgttt
     3421 ctgatccgca aatatacatc ctttggcaag gttgaagcca aattaatcaa ggtcagtaat
     3481 acagttatta cttcccagta acaggtgacc ggtacgaatc gcactgttcc tggaaatttg
     3541 tcatacggtt cattggtatc gttcgttgtc aaaagcgccc gcggggtatc tgacggaaac
     3601 ccgttccagg tttaggttta attgatttcc acattccggg cagcgccaaa caagcctttc
     3661 ctgtaaggcc gcagctgaaa aaatccgccg gttgggacag gacggacact ttaggatcat
     3721 gtaaccgtaa gcatctgcaa acggccagtt ggaggaggaa cacatacctt ca
//
LOCUS       DASVMB010000256         5867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_474096,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000256 DASVMB010000000
VERSION     DASVMB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474096"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF531_07515"
     CDS             complement(<1..314)
                     /locus_tag="VF531_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX7713857.1"
                     /translation="MSEIEAVVVAAGLSSRAGGWKPGFKIGNFTVIERCILGMVPFCS
                     RVIVIGGAQFHRLQGLLTKEKYPMVELFYNRDYRKGMLASAREGFRRVRTDRFFFIPG
                     DYP"
     gene            complement(703..1317)
                     /locus_tag="VF531_07520"
     CDS             complement(703..1317)
                     /locus_tag="VF531_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713858.1"
                     /translation="MKNMSTVIFWGALWGLAEATLGHLLHIWALPVGWLIWFPLAFGL
                     MGAVYRRTKQPLLVFLTAMVASAVKLIDFLMPVRLDYVINPAVSIVLEGLAVSVAYQL
                     GLLHGANRSVFWQIAAISFLWRVLYFGYLFLMSEMFLRVSPLRGIQPAAEFFLLETAA
                     NALLIYGMMKMPVKQRAGVTCPLTPHPALSLGLLTMACWIQWIW"
     gene            1500..2384
                     /locus_tag="VF531_07525"
     CDS             1500..2384
                     /locus_tag="VF531_07525"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX7713859.1"
                     /translation="MARIGLRNLKTALAVFICVVISKVARLEFTFFVAIAAIISMENS
                     LTNSIKAGKNRMAGTFIGAVIGLIGALIKPGSAIICGLGMIAVIYFCNLLRWRKPISI
                     AGVVFIAIMVSLGGKNPVQYSINRILDTFIGITVAVVVNYLFFPPDYIRRIRRIITEL
                     IAKTEQILYHLFIDGQNVGLIEYEQELAKGNELLELSCADSKLRKLRNAEEIPGIQKA
                     FGALNRIAKLLVILSQMNPGSNLNESGARELQKSFGWEHLHPGAVRDDETSIVFNYHL
                     REILTLYHSLKQTVAPNL"
     gene            complement(2550..4880)
                     /locus_tag="VF531_07530"
     CDS             complement(2550..4880)
                     /locus_tag="VF531_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7713860.1"
                     /translation="MSTAIGTSVPRKEAPDKVTGNVKYNSDLSSPDLLHGWMVTSLYA
                     HANIKSIDTSAALKAPGVKAVITGDYFPAMIGVLLADRPPLAIGKVRYFGEPLAVVVA
                     NSEAEAHYAADQVMVEYEPLTVVNSPGDAVQSGAPLVHEQLGSYTTIENTVYPEPGTN
                     IAHRQKIRKGDMAQGWAESEVIIEGSYKLPQSDHAAMETRSARARIQQDGTVIIHSSS
                     QAPFMIKTIISRTFHVGVGKIIVNTPLVGGAFGGKTSVQLEPIVYLASKAVGGRPVML
                     ANTREQDMASSPVQIGVEARVKLGATRAGKLKAAEMTFLIDGGAYTDSASIMAKAIAA
                     NCTGPYNVENLWCDSLCIYTNHTYATAFRGFGHTAYTFAMERAMEKMAYALGMDPFEF
                     RLRNVLVPGNLSPTQVRITQSNFGDLGKCLQKLKELSHWDEGRRTETGGSKVRAKGLS
                     CFWKTSSSPTDAISGVILILNADGTINLLCGAVECGQGTKTAVAQILAEKMKMLPDQI
                     HVAMEVNTRQNPDHWKTVASMSTYMVGRAVLEAADDVIRQIKDVAAAVMRCPVEDLDV
                     AGGKVFLRDDPAVFIEFKDLAHGYKYQDGNGIGGQIMGRGKFIMRHLNPPDQETGKGK
                     PGPGWTVGAQAVEVEFDQEDFTYRILKAITVVDAGKVINPMGARGMVTGAMSMGLGYG
                     SREALSYSDSGILQNPQFRMYKPLRYGEVQNYVVEFIETPELDAPYGERGLGEEGILG
                     MPSALANALSTAAQVELNELPLVPERIWKAKGGPLG"
     gene            complement(5001..>5867)
                     /locus_tag="VF531_07535"
     CDS             complement(5001..>5867)
                     /locus_tag="VF531_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7713861.1"
                     /translation="PRSIPAGAVFTTENPRVVRLTQGVMAKVNFGVKLPPEQPLVQDS
                     PVSQAAAAPPEIPEPEIQAPALPDAPAVAESEPPAPDVHVSTQKGFNNGSVLITMEGA
                     EILPGIDVKLIGSGETTISGTIINNQTGKMEAFFDLKGQPEGTYNYYIFYPDGHSVKL
                     GSDFEVRKFSPELFHPQTLVPVYFDLNKSNIRANQTANIAKDLVTLLKNPGAGIILGG
                     YTDSRGSAVYNLGLSLRRATAVKQYLVEHGVKPEQIVIYAYGKELAKQGTSENVWQND
                     RRVDVIIYEDKQ"
BASE COUNT         1405 a         1594 c         1443 g         1425 t
ORIGIN      
        1 gggtaatcgc cggggataaa aaagaaccgg tccgtcctga ctcgccgaaa cccttcccgg
       61 gccgaagcga gcattccttt ccggtaatca cggttgtaaa acaactccac catgggatac
      121 ttctccttgg tgagcagccc ctggagccgg tgaaactgag cgcccccgat tacgataacc
      181 cgggaacaaa acggaaccat ccccaaaata cagcgctcaa tgacggtgaa gttcccgatt
      241 ttgaaccccg gcttccatcc accggcccgg gaggaaagcc ccgccgctac gaccacggct
      301 tcgatctcac tcaagtatta taatcatcct ttcacggtca tttttcgtgt actttagtgg
      361 atttcgcggt taataaaccg tcttttttcg cgcgaaagtc acaaaaaaat ccatgctgca
      421 attaaggcta ttcccatttt cgtgcctttt gtgtgtttcg ttgtaataag ataggtttac
      481 agatagaact ttttaaccac gaaaatcacg aaatacacga aatgtctctc ttcaggcaat
      541 tgttagccca aaatgcggaa accttttgct tatgatagat aaaaatgctt caattaaaaa
      601 gcttaaccgt cctcattctt tcgtgcactt tggtgtgttt cacggttaat taaaccgtct
      661 tttttcgcgc gaaagtcacg aaagagaata attattctta cgctaccaga tccactggat
      721 ccaacaagcc atcgtcaata atcccaacga tagagcggga tggggagtga gagggcaggt
      781 aaccccggct cgctgtttga cgggcatttt catcatcccg tagatgagga gggcatttgc
      841 ggcagtttca agcaggaaaa actcggccgc cggttggatc ccgcgaagtg gcgatactcg
      901 gagaaacatt tccgacataa ggaagagata cccgaaatat agtacccgcc ataaaaaact
      961 tatggctgca atctgccaaa acacgctccg gttggctcca tgtaacagtc ccaattgata
     1021 agcgacgcta accgccaggc cttccagaac aatcgatact gccggattaa tcacataatc
     1081 gaggcggacg ggcatcagaa agtcgatcag cttcactgct gatgccacca tagcagttag
     1141 gaaaaccaaa agcggttgct tggtgcggcg atagaccgcc cccatcaagc caaaggccaa
     1201 gggaaaccag atcagccaac cgacgggcaa ggcccaaatg tgcaacagat ggccgagagt
     1261 ggcttcggcc aacccccaca atgcccccca aaagattacc gtactcatat ttttcaaggt
     1321 taccaccccc gatcgagttt ccggcaaggt aaccctatgc gtatccccgg cgaaatatac
     1381 ccgaagcctc gcatccgtag taaaagctcc aacccggatt gggtttataa aataatcctt
     1441 cggagaaaaa tagcacccat caggaacaca cctgagataa ataagaaaga agcagaaaat
     1501 tggcacgaat tggattacga aatctcaaaa ccgccttggc ggtcttcatc tgcgtggtca
     1561 tatcgaaagt cgccagactg gaatttacct tttttgtggc gattgccgcc atcatctcca
     1621 tggagaattc gttgaccaac tcgatcaaag ccggaaaaaa ccggatggcg ggtaccttca
     1681 tcggggcggt aattggtttg attggcgcct taatcaagcc gggaagcgcc attatttgcg
     1741 ggttgggaat gattgctgtc atttatttct gtaatctgtt gcgatggcgt aaaccgatat
     1801 ccattgccgg agtggttttc atagccatca tggtgagctt gggcggcaag aaccccgtac
     1861 aatacagcat caaccggatc ctggatactt ttattggcat cacagtggca gttgtcgtta
     1921 attatttgtt tttccctccg gattatatca ggagaatccg ccgcatcatc accgagttga
     1981 tcgccaaaac agaacagatc ctatatcatc tgttcattga tggccaaaac gtaggcttaa
     2041 tcgagtatga gcaggaactt gccaagggga atgaattatt ggagctttcc tgtgcggaca
     2101 gtaagttacg gaaattaagg aatgcggaag agatccccgg gattcaaaaa gcctttggcg
     2161 ctttgaatcg aattgccaag ctcctggtga ttctctccca aatgaatccg ggttccaatc
     2221 tgaatgaatc cggcgcccgc gagctacaaa aaagtttcgg ctgggagcat ttacaccccg
     2281 gtgcagtccg ggacgatgaa accagtatcg tctttaacta tcacctccgg gagattctaa
     2341 ccctttacca cagtctcaag cagactgtcg caccgaatct ctgaacattt cccaattttt
     2401 ataacccatc ccgagttgat tgattcgtag agtatggtat acctgttctt cgatggcttc
     2461 tctcgtatat caaaacggct tcactgcggc ttgaatctct ccgtaacttg tgcaacggca
     2521 tagatttgac tggagccatt cttcgatgat caacctaacg ggcccccttt cgctttccaa
     2581 attcgttccg gaaccaaggg cagctcattc aattcaacct gtgcggcggt ggaaagggcg
     2641 ttagccagag cggatggcat gcccagtatc ccctcctcgc ccaaaccgcg ctcaccgtat
     2701 ggcgcatcga gttcgggagt ctcgatgaat tcgaccacat agttttgaac ctctccatac
     2761 ctaagcggct tatacatccg gaactgcggg ttctgtaata ttcccgagtc actgtaggat
     2821 aacgcctccc ggcttccgta accgagtccc atgctcattg cgccggtaac catcccccgg
     2881 gcgcccatgg gattgatgac ctttcccgca tcaaccacgg tgattgcctt aagaatccgg
     2941 taagtgaagt cttcctgatc aaactcgacc tctacggctt gggctccgac cgtccagccc
     3001 gggccgggtt tcccttttcc ggtctcctgg tcgggggggt tcaaatggcg catgatgaat
     3061 ttgccccgac ccatgatttg cccgccgata ccattgccat cctggtattt atagccgtga
     3121 gccagatcct tgaattcgat gaaaacggcg gggtcatcgc ggagaaaaac cttccctccg
     3181 gcgacatcca aatcttctac cgggcagcgc atcaccgccg cagccacatc ctttatttgg
     3241 cggatcacat catcggctgc ctccagcact gcccgtccca ccatgtatgt gctcatactg
     3301 gccacggtct tccagtgatc cggattttgt ctggtattta cctccatggc cacatggatc
     3361 tgatccggca gcatcttcat cttttccgcg aggatctggg ctacggcggt cttagttccc
     3421 tggccgcatt ccaccgcccc gcacagtaga ttgatggtac catcggcatt caagataaga
     3481 ataacgccag agatggcatc tgtcgggctg ctggaggtct tccaaaaaca gctgagcccc
     3541 ttggctctca ctttagaccc gccggtttca gtacggcgac cctcgtccca atggctcaat
     3601 tccttcaatt tctggagaca cttgcccaaa tccccgaagt tgctttgggt aatccggacc
     3661 tgagtcgggg agagatttcc cggaaccagg acatttcgga gtcgaaactc aaacggatcc
     3721 attcccaggg cgtaggccat cttttccatg gcccgttcca tggcaaaggt ataggccgta
     3781 tgtccaaacc cccggaatgc cgtggcgtag gtgtggttgg tataaataca caaggaatcg
     3841 caccagaggt tttccacatt atatggcccg gtacagttgg cagcgatggc tttggccata
     3901 atcgacgcgg aatcggtata ggcgccgccg tcgatcaaga atgtcatctc tgccgctttc
     3961 aacttgcccg cccgggtggc tccgagtttc acccgggctt ccactccgat ttgtaccggt
     4021 gaactcgcca tatcctgttc acgggtgttg gccagcatca ccggacggcc gcccacagcc
     4081 ttggaagcga ggtagacgat gggttccaat tggacactgg tcttgccgcc gaaggccccg
     4141 cccacgagcg gggtattgac gatgatcttc cctaccccaa catgaaaagt acggctgata
     4201 atggtcttaa tcataaaagg agcctgggag gaggaatgaa taattaccgt cccgtcttgc
     4261 tgaatccggg cccgggccga tcgggtctcc atggcggcgt gatccgattg gggaagttta
     4321 tagcttcctt cgatgatgac ttcgctctcc gcccaacctt gggccatgtc gcccttgcgg
     4381 atcttttggc gatgcgcgat attggtaccg ggttccgggt aaaccgtatt ttcgatcgtc
     4441 gtgtaggaac ccagttgttc atggacgagg ggcgctccgc tttgaacggc atctcccggg
     4501 gaatttacca ctgttaacgg ttcatactca accatgacct gatcggccgc ataatgcgcc
     4561 tcggcttcac tattggccac gactactgcg agcggttccc cgaaataacg gactttcccg
     4621 atggcgaggg gcggccgatc cgccaggagc actccgatca ttgccgggaa ataatccccg
     4681 gtaatcacgg cttttacccc cggagctttt aacgccgcgg aggtatcaat cgacttaata
     4741 ttagcgtggg catacaagct cgtcaccatc cagccgtgga gcagatcggg actggagaga
     4801 tcggagttat atttgacatt tccggttacc ttatccgggg cttccttcct gggaacgctg
     4861 gtgccgatgg cggtagacac tggtgctcct tgacggattt agagttttta taatattacc
     4921 caacgaaagg ggttcatatt ctacccttat ccgccccggg gcgacctctt tctcccaaca
     4981 aatacaatgt caatgatgga tcattgctta tcctcataga tgatcacatc aaccctgcgg
     5041 tcgttttgcc agacattttc gcttgttcct tgtttggcaa gttccttgcc ataggcgtaa
     5101 atcacgattt gctcgggctt aaccccatgc tcaaccagat attgtttgac tgcggtggcc
     5161 cggcgcagtg aaagacccag attatatacg gcactgcccc gcgaatcggt atatccgccc
     5221 aagatgatgc cggctcccgg atttttcaga agggtcacca gatctttcgc gatgttggcg
     5281 gtttggttgg cgcgaatatt tgatttgttc agatcaaaat agaccggcac cagcgtctgg
     5341 ggatggaaaa gttccgggga aaatttacgg acttcaaagt cactccccaa tttgacggaa
     5401 tggccgtccg gataaaagat atagtagtta taggtccctt ccggctggcc cttgaggtca
     5461 aagaaagctt ccatcttccc cgtttgattg ttgatgatag tcccggaaat cgtggtctct
     5521 cccgaaccga taagtttgac gtcaattccc ggaagaattt cagccccttc catcgtgatc
     5581 aagacagagc cattgttaaa tcccttttgc gttgaaacat gaacatccgg ggcaggcggt
     5641 tcagactccg caactgccgg agcatcggga agcgcgggcg cttgaatctc cggttccggg
     5701 atctcaggcg gtgcggctgc cgcttgcgag accggggaat cctggaccag cggctgctct
     5761 ggaggaagct tgacaccgaa gttcaccttg gccatcacgc cttgggttag acgaaccact
     5821 cgcggattct cggtggtaaa gaccgccccg gccggaatcg atcgagg
//
LOCUS       DASVMB010000257         7703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_253923,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000257 DASVMB010000000
VERSION     DASVMB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253923"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /gene="thrS"
                     /locus_tag="VF531_07540"
     CDS             <1..1564
                     /gene="thrS"
                     /locus_tag="VF531_07540"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7713862.1"
                     /translation="VKEDLPLVRSTSGREDAIRRMTEKGEPYKVELIRDLPVDAEISF
                     YRQGDFIDLCAGPHLESTCKVRAFKLTQVSGAYWRGNEKNKMLQRIYGTAFPEQHEVD
                     EYLARVEEAKLRDHNKLGRELELFTTAEYIGQGLPLLMPKGAKVVQLLQRFVEDEEER
                     RGYLLTKTPFMAKSDLYKISGHWQHYKEGMFILGDEEKDEEVLALRPMTCPFQFTIYN
                     AKLHSYRELPIRYGETSTLFRNEASGEMHGLIRVRQFTISEGHLVVTPEQLEAEFKGV
                     LDLIHYMLGTLGLEKDVWYRFSKWDPRNKEKYIDNPAAWEDTQAKMKTILDHLGVEYQ
                     EAEGEAAFYGPKLDIQFKNVYGKEDTIITVQIDFSLPERFDMTYIDKDGAKKRPYIIH
                     RTSIGCYERTLAMLIEKYGGAFPTWLAPVQVKIMPIMESHHAYAEEVRSRLMGVGIRS
                     EIDYRNEKIGYKIREAQGEKIPYMFVIGGKELENHTVAVRSRKDGDLGPMPVDAVIER
                     ITAEVQERSLTV"
     gene            1903..3285
                     /locus_tag="VF531_07545"
     CDS             1903..3285
                     /locus_tag="VF531_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7713863.1"
                     /translation="MRFDFLHQDTEVRRNMILNGSIPKTLLFLSVPTLMMGIVQSIIP
                     VVDGLFINNIAGVVAASAISYSGPIINMMGALAQGLSVAGMAIIGQTNGRGDFEDAKK
                     ISAQIVVFAFLLGLITAPILILVAYPVAGHVYAEISHDVFVYIALNALVIPFAFLESI
                     YNAIKNAAGKPEAAFVRMLLMLVLKVLSNTLFIAVFRWGIVGAVMASLVANILICIWM
                     YYELFVKVGEDRLEIKGFKFDFDVIRELFRIGIPSMLSSLILNLGFFLINNEVAKYGT
                     IVMNGQGIASNITSVCFILPSSFGSSVTTMVSMNIGAGQSEKAKASCTVGCIISAITA
                     AVLIAIVVPLSSYITVLFTRQQDVLDVANKALHIYTYSVIGFGICMVQQGAFIGLGRT
                     GIPLFASILRIWLLRYLFILATEHVLGPYSVFWGNLFSNYAAALITTILILRTQWVTA
                     VPRKAISVEA"
     gene            3386..4303
                     /locus_tag="VF531_07550"
     CDS             3386..4303
                     /locus_tag="VF531_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7713864.1"
                     /translation="MNSQLKNNQGLTEAEFLQSYDATKYERPSVTVDILLFTVQDTTA
                     ENYRKLPEKVLKLLLIKRGDHPFLGKWALPGGFVNMDESLDEAALRELKSETNAGDIY
                     LEQLYTWGEPRRDPRTRVISCSYMALVDSSALRIKAGDDAADARWFTLKRSLVEEQRF
                     ITENGYRFQQTFDLKLDSKEESLSAKIRTVKSVEDNRSGFNREILNSEGIAFDHASII
                     DYGIDRLRNKLEYTDIVFNLMPQYFTLTELQQVYEIILEKELLKANFRRKIAPMVVET
                     PRLTQDEGHRPAKLYRFNPGWQERANLSV"
     gene            4316..6391
                     /locus_tag="VF531_07555"
     CDS             4316..6391
                     /locus_tag="VF531_07555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7713865.1"
                     /translation="MLRSLERINRYQQGDLTVLKTDPWLMHLALAEEVHINLERVDSI
                     EELRRNLVLDYVARTLSILEGLDLPEAETHLLEEVLIWAEVAKGGMLHQRREWLKKGY
                     NLYVHNIGSAQIYQAEMSQLPTGVDSRLISTLICTHGLIGQYIRGEVPLEKNRPLTDL
                     VSQKITDSESLYRTLVNLNHCIIAGVSPELWKAIQKETQAVIRMITHGNYEQEYSLTV
                     RLKKLRGLSIQNGEDFSKEVERVLKDSRVVDAFSQILAGTDLWFVEAALYDLSFEEFV
                     KVFLLIYKAIDLSQVRHISFEKLMKGLYYQHEGRKRINIYKKRVIEKYLAGLSIPGIL
                     EGITNPSPHLEHKIEMNEAADTVFFDFQFSAAGAKLIDFCTEAEKADLLYERAIVLLF
                     DLFELRRDKYDRFLEEEKYLVTMNRGVDYKKIILDYVTGQEIVDIGPGGGILLDLIEL
                     NYPDKKVTGIDISQNVLDALERKKKTEGRKWQVIYGDALNLSEYLKPGSVDTVIFCSI
                     IHELYSYLEFEGRSFNIKTIAAALQSAFTVLAPGGRIIIRDGIMTDPVDQKRIIKFLS
                     ADGVKFLTRYASDFKGRKIEYRLIGLNEVLMPVNDAMEFLYTYTWGEKSYAHEINEQF
                     GYFTPGTYRAFIEETLGPTARIIECRHFLQEGYTTALSSKIEFFDERHIPVPLPDSTC
                     LIVVEKRQK"
     gene            6438..7448
                     /locus_tag="VF531_07560"
     CDS             6438..7448
                     /locus_tag="VF531_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7713866.1"
                     /translation="MFQFIKFEPNDYVLRYRNGKVAQEGSGLSFFYYAPITSLVKVPM
                     GSAEVPFIFEELTIDFQTVTVQGQVTYRIVDPKKIVKLLNYTLDSSGRRYITEDPSKL
                     PPKVINIARVLTKKHLETMTLKESLKSSESLAQSIADEIKKNNEINSLGLEILGLMIL
                     AILPNKETGRALEAQTREEILRRADEAVYERRNSAIEQERVIKENELNTDIAVENKKR
                     QIRETQMEAERVIQEKQNELKADQMNFEIGMEEKRKDLIGLEAENDRTKADAKAYELS
                     GIMKAMNGMEPRMIQALASMGMQPDQLIALAFQGLADKAEKIGQLNITPDLLQDLMNK
                     AE"
     gene            7504..>7703
                     /locus_tag="VF531_07565"
     CDS             7504..>7703
                     /locus_tag="VF531_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7713867.1"
                     /translation="MARTEDVKIILVKRNTRLEDLVSRYNTVSQVKFYIEHLGADFSD
                     YQTEDNKYKQVVAETERILEGFG"
BASE COUNT         2061 a         1688 c         2042 g         1912 t
ORIGIN      
        1 tgtcaaggag gatctaccgc tggtacgatc gacttccgga cgggaggatg ccatccgccg
       61 gatgaccgaa aaaggggaac cgtataaagt ggagctcatt cgggatctcc ccgtggacgc
      121 cgagatatcc ttctaccggc aaggcgattt catcgacctt tgcgccggtc ctcaccttga
      181 gtcaacctgc aaggtccggg ccttcaaact gacccaggtc tctggagctt attggcgggg
      241 taatgaaaaa aataagatgc tgcaacggat atacggaact gcctttcccg agcagcatga
      301 agtcgatgaa tatctggccc gggtcgagga ggccaagcta cgggaccaca acaaacttgg
      361 cagggaattg gaactcttca ccactgccga atatatcggg caggggttgc cgttattgat
      421 gcccaaggga gccaaggtgg ttcagctctt gcagcggttc gtcgaggacg aggaggagcg
      481 ccgcgggtac ttgttgacca agaccccctt tatggccaag agcgatctat ataaaatctc
      541 cgggcactgg cagcattata aagaaggcat gttcatcctg ggagacgagg aaaaagacga
      601 agaggtactg gcgctgcggc ctatgacctg ccccttccag ttcaccatct ataatgccaa
      661 acttcacagt taccgcgaat taccgatccg gtacggggag acctcgacct tattccggaa
      721 tgaagcttcc ggagaaatgc acgggttgat ccgggtccgg cagtttacca tctccgaagg
      781 ccatctggtc gtcacccccg aacagttgga agcggagttc aaaggggtgc tggatctgat
      841 ccactatatg ctggggaccc tggggctcga aaaggatgtc tggtaccgtt tctccaagtg
      901 ggatccgcgc aacaaggaga agtatatcga caacccggcc gcttgggagg atacgcaggc
      961 aaagatgaaa acaatcctgg atcatctggg ggtcgagtac caagaggctg agggcgaggc
     1021 ggcgttttac ggaccgaagc tggatattca gtttaagaat gtttacggta aagaggatac
     1081 catcatcacg gttcagatcg atttctccct gccggaacgg ttcgatatga catatatcga
     1141 caaggacggc gccaagaaac ggccctatat cattcaccgg acctccatcg ggtgctatga
     1201 gcggaccctg gcgatgctca tcgagaagta cggcggagcc ttcccgactt ggctcgcgcc
     1261 ggtgcaggtc aagatcatgc cgatcatgga gagccatcac gcttatgccg aagaggtacg
     1321 gagtcgcttg atgggcgtcg ggatccgctc ggagattgac taccgcaatg agaagatcgg
     1381 ttataagatt cgcgaagcgc aaggggagaa gattccgtat atgttcgtca tcggcggaaa
     1441 ggaactggaa aatcacactg tagccgtacg ttcccggaag gacggcgatc tggggccgat
     1501 gcctgtcgat gcggtgatcg agcggattac cgcggaggta caggaacgga gcttgacggt
     1561 gtaatcgtgg tgacggttta acagtataaa agaattcgaa gaaaccccgg cgaaactgcc
     1621 ggggttttaa aaagtttgaa ttaagaagtg tgaagttaga aatggttttt aggttttgtt
     1681 tcacacttcg cacttcacac ttctaggttg gtggcggtta gccatgatat atgttaacca
     1741 tatagagaaa acagctatat atcactttat tttacaatag attcctgtta aacgggattt
     1801 attcagtatg aaaaatgtgc tactatttaa gattgtacag tgaaatatag agatatagcc
     1861 tgtggtaaac ataccattgc cccgtagaaa gctggcatat tcttgcgctt cgatttttta
     1921 catcaggata ccgaagtaag acgcaatatg atattgaacg gatcgatccc caaaaccctt
     1981 ctatttctat cggtccccac attgatgatg ggcattgtcc aatcgatcat accggttgtg
     2041 gacggattgt tcatcaacaa catcgccgga gtggttgcgg ctagcgccat ctcatactcc
     2101 ggccccatca tcaacatgat gggcgctctg gcgcaaggcc tgagtgtggc ggggatggcc
     2161 atcatcggcc aaaccaacgg caggggcgat tttgaggacg ccaaaaagat ttccgcccag
     2221 atagtggtct ttgcgtttct tttaggactt attaccgctc cgatactgat attggtggca
     2281 tatccggttg ccggacatgt ttatgcggaa atatcccatg acgtttttgt ctatattgca
     2341 ctcaatgcat tggtgatacc cttcgcattc cttgagtcga tctataatgc gatcaagaat
     2401 gcggcgggca agccggaggc cgcgtttgtc agaatgcttt tgatgctggt attgaaggta
     2461 ttgtccaata ctttgttcat tgcggtattt cgttggggca tcgtcggtgc cgtaatggct
     2521 tcactcgtag cgaatatttt gatctgcatc tggatgtatt acgagctgtt tgtgaaggtg
     2581 ggcgaggacc ggctggaaat caagggcttc aaattcgatt tcgatgtcat ccgggagctt
     2641 ttccggattg gaattccgtc gatgctctcc agtctcattc tcaatctcgg ctttttcctc
     2701 atcaataatg aagtggccaa gtacggaacc attgtcatga acggtcaggg cattgccagt
     2761 aatattacct cggtatgttt tatcctcccg tcatcattcg gttcttccgt caccaccatg
     2821 gtcagcatga acatcggagc cggacagagc gagaaggcca aggcctcctg cactgtgggc
     2881 tgcatcatca gcgccattac ggctgcggtc ttgatagcga ttgttgtccc attatcctcg
     2941 tatatcacgg ttttgtttac aagacaacag gacgtgctcg acgtagccaa caaggcacta
     3001 catatttata cctattccgt catcggtttc ggtatctgca tggtccagca aggcgccttc
     3061 atcgggctgg ggcgtacagg gatacccctg tttgcaagca ttcttaggat ttggctcttg
     3121 aggtatcttt tcatacttgc gaccgagcat gttttaggcc catactcggt attctgggga
     3181 aatctctttt caaattacgc tgctgcgctg atcaccacca ttctcatttt gcggactcaa
     3241 tgggtcactg ccgtacctcg taaggcaatc agcgtggaag cgtaaacatt taggaaaatc
     3301 ctgtttgact ttatccaatg atagtactat aatgataata acaaaatgtt attaacaaga
     3361 tgagaccaag ttttggggtg gagccatgaa cagccaatta aaaaacaacc aagggctaac
     3421 ggaggcggag tttttacaaa gttacgatgc gaccaagtat gagcggccgt cggttaccgt
     3481 tgacatcctt ttatttacgg tacaggatac cacggcggag aactaccgca agctaccgga
     3541 gaaagtactc aaattattgc ttatcaagcg gggagaccac cctttcctcg gcaaatgggc
     3601 tttacccggg gggtttgtca atatggatga aagtcttgat gaggctgcgt tacgtgagct
     3661 gaaaagcgaa accaatgccg gggatatcta cctggagcag ttatatacct ggggtgaacc
     3721 ccgccgcgat ccccggaccc gggtgattag ttgctcctat atggcgctgg tcgacagttc
     3781 ggccttgagg attaaggctg gagatgatgc cgcggatgcc cggtggttta cgctcaagcg
     3841 aagtctggtg gaagaacaaa ggttcatcac ggaaaatggg taccggtttc aacagacatt
     3901 tgatctaaag ctggattcga aggaggaatc cttatcggcc aaaatacgga cagtgaagag
     3961 cgttgaggac aaccgatccg ggttcaaccg ggagatcctt aattcggagg ggattgcgtt
     4021 tgatcacgca agcattatcg attacggaat cgaccggtta cggaataaac ttgaatacac
     4081 cgatattgtc ttcaatctga tgccgcaata tttcaccttg accgagttac aacaggtata
     4141 tgagattatt ctcgaaaaag aattgctaaa ggccaacttc agacgaaaga ttgcgccgat
     4201 ggtcgtggaa actccccgcc tgactcagga tgagggtcac cggccggcga aactatatcg
     4261 attcaacccg ggttggcagg agagagcaaa tttatctgtt taaggagaaa acgagatgct
     4321 acggtctttg gaacgaatca accggtatca acaaggcgat ctgacagtgc tgaaaaccga
     4381 cccctggtta atgcatctgg cactcgccga agaggttcat attaatctgg aacgggttga
     4441 ttccatcgag gaattacgga gaaatttagt cttggactac gtggcgcgaa ccctttcaat
     4501 tcttgaggga ttggatttgc ctgaggcgga aacccatctg ttggaagagg ttttgatctg
     4561 ggctgaggtc gccaagggcg gtatgctgca ccaacggcgg gaatggctca aaaagggata
     4621 taacctgtat gtccataata tcggctccgc tcaaatctac caggctgaga tgtcgcaact
     4681 gccgaccggt gtggattccc gattgatttc aaccttaata tgcactcacg gattgatcgg
     4741 ccagtatatc cggggtgaag taccgctaga aaagaatcgg cctttgaccg acctggtgtc
     4801 ccaaaagatt accgatagcg aaagcctata tagaactctt gtcaatctca atcattgcat
     4861 catcgccggt gtatctccgg agttatggaa ggcaatccaa aaggaaaccc aggcggtgat
     4921 ccggatgatc acccatggca actatgaaca agaatattcg ctaactgtaa ggttaaagaa
     4981 actacggggt ttatccattc aaaacgggga ggatttctcc aaagaagttg agcgggtctt
     5041 aaaagattcc agggttgtgg atgctttttc gcagatcctg gccggaaccg acctatggtt
     5101 tgtggaggcg gctttatatg atcttagctt cgaggagttc gtcaaagttt ttttgttaat
     5161 ctataaagcg attgatctgt cgcaggttcg ccatatcagt ttcgagaaat taatgaaagg
     5221 actctattac cagcacgagg gaaggaaacg gattaatatc tataaaaaaa gggttatcga
     5281 gaagtatctg gccgggctat ccattcccgg aatacttgag ggaataacaa atcccagtcc
     5341 tcatctggaa cataagattg agatgaatga ggcggctgat acggtgtttt ttgatttcca
     5401 gttctccgcc gccggcgcta agttgataga tttctgcact gaggctgaaa aagctgatct
     5461 tttatatgag cgggccattg ttctattgtt tgacctgttt gagcttcggc gggacaagta
     5521 cgatcggttt cttgaagaag agaagtatct cgtcaccatg aatcgcggcg tggattataa
     5581 gaagatcatc ctggattatg taaccgggca ggagatcgtc gatatcggcc cgggcggcgg
     5641 catcctgctg gatttgatcg agctaaacta cccggataaa aaggtcaccg ggatcgatat
     5701 ctcccagaat gttttggatg cattggaacg caagaaaaaa accgagggcc gcaagtggca
     5761 ggtaatctac ggagatgcgc taaacctctc ggaatatctt aaaccgggaa gcgtcgatac
     5821 ggttatcttt tgttccatca tccatgaact ctattcttac cttgagtttg aaggccggtc
     5881 ttttaatatt aaaacaattg ccgcggcttt acaaagcgca tttaccgtcc tggctcctgg
     5941 agggaggatc atcatccgcg acgggattat gaccgatcct gtggatcaaa agcggatcat
     6001 caaatttctc tctgcggatg gcgtgaaatt cctcacccgg tatgccagcg attttaaggg
     6061 aagaaagatc gaatatcgac tcatcggctt aaacgaagtt ttgatgccgg tcaatgatgc
     6121 gatggagttt ttatacacat acacctgggg tgaaaaatca tatgcccatg agatcaatga
     6181 acagtttggc tatttcaccc cggggactta ccgggctttt attgaagaga ctctgggccc
     6241 aacggcccgg atcatcgaat gccggcattt tctccaggag ggctatacca ctgccttatc
     6301 cagcaaaatt gagttttttg atgagaggca tattccggtt cccttaccgg acagcacttg
     6361 cttgattgtg gtggaaaagc ggcaaaaata gccgttcaat cgatataagg gaggtgacgc
     6421 gcgcgagcgc gcggatcatg tttcaattta tcaagtttga acccaacgac tatgttttaa
     6481 ggtatcgtaa tggaaaggtt gcccaggaag gttccggatt atcgtttttc tactatgccc
     6541 cgattacatc ccttgttaag gttccgatgg gtagtgccga ggttccattc atttttgaag
     6601 agttgactat agactttcag actgtaaccg tgcaaggtca ggtcacgtat aggatagtcg
     6661 atcccaagaa aatcgtgaag ctgctaaatt ataccttgga tagcagcggg cgtcgctaca
     6721 ttacggagga tccctccaaa cttcccccaa aagtaatcaa tatcgccaga gtcttgacaa
     6781 agaaacacct ggaaaccatg accctcaaag aaagtttaaa gtccagcgaa agtctggcgc
     6841 agtccatcgc ggatgagatt aagaaaaata atgagataaa ctcgttgggt cttgaaattt
     6901 tgggactgat gattttggcc attttgccca ataaggagac gggtagggct ttggaagccc
     6961 aaaccaggga agagatcctt cgccgggccg atgaggccgt ttacgaacgg cgtaattccg
     7021 ccatcgaaca ggaacgggtg atcaaggaga atgaactgaa taccgacatt gccgtagaga
     7081 ataagaaaag gcagatccgc gagacccaga tggaggcgga aagggtcatt caggaaaagc
     7141 agaatgaact aaaagcggac caaatgaatt ttgaaatcgg tatggaagag aagcgaaaag
     7201 atctgattgg attagaggcc gagaatgaca ggaccaaggc ggatgccaaa gcgtatgagt
     7261 tgtcgggaat catgaaagcc atgaatggca tggagcctcg aatgattcaa gcattggcca
     7321 gcatgggaat gcagccggac cagttaattg ccctggcttt tcaaggtctg gccgacaagg
     7381 cggaaaagat tggacagtta aatattaccc cggatctatt acaagatttg atgaataagg
     7441 ccgagtaaca tagttacggt caggcgctgg tttgggttgg aagtagaatg gaggcgcttg
     7501 ctcatggcgc ggactgagga tgttaaaata atcttggtaa aaaggaatac gcggctggaa
     7561 gatctggtca gccggtataa tacggtttct caagtcaagt tttatattga acatttggga
     7621 gccgattttt ccgattatca aactgaagat aataagtaca aacaggtagt tgccgaaacg
     7681 gagcggattc ttgaaggctt tgg
//
LOCUS       DASVMB010000258         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_399249,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000258 DASVMB010000000
VERSION     DASVMB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399249"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VF531_07570"
     CDS             <1..1350
                     /locus_tag="VF531_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713868.1"
                     /translation="KGVQPSGAGFIENRVAAEIKAWLQTEKRPWQACLITDGGLDLNG
                     RELTGVFRGAWRTVMVGRNGNDLGVTGLRLLPGPRAQFLVYNGKSRTEKIKVTLKFNE
                     KPIITTRLEAAPGVTRRILPFGGTVRPGIYEVESSATGDNLTWDNSYRLALNPQRRFR
                     VLLIGATDPFLNAALRDPRVQLARLPQFPASGLTGSDWDLVVADRVKIPAGLQVNLLA
                     IGTIPEGAPVRLGPSVSGVLTGIDTSHPLARFVDWGSVRVGGGNSLEIASNVQPLATV
                     GGQPIMAVWEQAGYRSVVIGTDLFHSDLALSGAFPVFFQNLLLWCVPQAANSLAYNLT
                     VGEPVSISEGRDWRFLDTRGIRVVRNGSMLSLRAETTGVFRWQSGKTKGVLVSNIAPS
                     ELQIGPRILVTQKSKVRTAAGYEVNRQSLADYFTLLFLILLCLEWICWSGGWRMIKE"
     gene            1357..4164
                     /locus_tag="VF531_07575"
     CDS             1357..4164
                     /locus_tag="VF531_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7713869.1"
                     /translation="MWWNRPIWLLAILLMIPILWPELLLRWRFKNQSLEALSGHSPRS
                     SNGLRKLSRLRAIAICAAILSLAGTGILIPTKNRQVVALVDVSASISPAQVEVSRGAL
                     LELLGRFNPGDRVAVIAFAGEPETIASFETPARLSMMLKTAALKAPRTGATNLEAAIR
                     LGSRMLSEKGGNRCMILLSDGRPTVGGMDLPAMVRESDIPIYVLGVGTSGSSLITREL
                     RVPEVIHPQERVLVRWEVESDRVQNVIATILMDNRTVGREPVKLAAGRNTVLLPVQVK
                     DPGIHRLDIRISDTAGKVLNQADSASLLNVTGPARVLVIHGDSPNSPITRALQTQGMQ
                     LTEEGVAGLPENANGLAGYGAVVLDNIPALYLSQGQQDALQGYTAGGGGLLVVGGDAS
                     LGRGEYYATGLEDLLPVQTDTRQRLFFSRANILFVIDHSGSMSEMVGKTSKQMAAMQG
                     VAAAIEELNPQDRVGILTFDVEPTWVLRFTPAGQKNQIRRSLSRIGQGGGTDMSTAME
                     EVVKGFSSVGPVRRHVVILSDGQTTNADFNKICRKLKNLGVTITTIGIGEEVNETLLK
                     DLARWGDGRYYRAQLDQIPTVIRKETVRVTRDQIQEGMFYPKIKTAAPFLEVTAWNRF
                     PVKGYLITKPKSMATVFLEIGKQDPLLASWRYGNGRVAVFTSDSGGRWLSSWSGRPNF
                     NQLWSQTIRSIERAAMDTGLRVTARAEAAAAVITVEAVGADGRLRPGLQLTGAALDDP
                     PSGATNAGTANITGDNLVFTETAPGHYEARVPLSGSGLKQFQVREAQSGEWSIGWLWN
                     PPGQEKLSTGPDRSFLGNIAAAAGGSLLTAANPVLPLPGWAWEKVPLQNWLILAALVC
                     FLVELCIRSTSLGQMTMARAVFASWWEAQRRIISTIRGSRTEQEEREKQSRDNIYEAY
                     RYLAERARRQREEDQKNAQIH"
BASE COUNT          894 a         1057 c         1288 g          946 t
ORIGIN      
        1 aagggggttc aacccagtgg cgctggtttc atagagaacc gggtggccgc ggagatcaag
       61 gcctggctac aaacggagaa acgtccctgg caggcctgcc tgatcacgga cggcggtttg
      121 gatttgaacg gtcgtgaatt gaccggggtt ttccggggcg cctggcgcac cgtaatggtg
      181 ggaaggaacg gaaatgatct gggcgtcacc ggacttcgtc tattaccggg gccacgggcc
      241 cagttcctgg tgtataacgg taaatcaagg actgaaaaga tcaaggtcac cctaaaattt
      301 aatgaaaagc ccataatcac tacccggctt gaagcggcgc cgggtgtgac ccgccggata
      361 ctgccttttg gagggacggt aaggcccggg atatatgaag ttgagagctc ggcgaccgga
      421 gataacttga cttgggacaa cagttatcgg ttggcgctca atccgcaacg ccgtttccgg
      481 gttttgctga tcggtgcgac agacccattt ttgaatgcgg cattacggga cccaagggta
      541 caacttgcca ggttgccgca atttccggct tccggcctga ccggctcgga ctgggatctg
      601 gtagtggccg atagagtaaa gatccccgcc gggttgcagg ttaatttatt agccatcggc
      661 acaattcccg aaggggctcc ggtccggttg ggtccctctg tcagcggggt tttgaccggc
      721 atcgatacca gtcatccttt ggctcgtttt gtagactggg gctcggtccg ggtgggtgga
      781 ggcaactccc ttgagattgc atccaatgtt caacccctgg ccacggtggg cggccaaccg
      841 attatggccg tttgggagca agcaggttac cgttcggtag tgatcggcac cgatctgttt
      901 cattccgatc tggcccttag cggagcattc ccggttttct tccaaaatct gctgctgtgg
      961 tgcgtgcctc aggcggccaa ctccctggcg tataatctga ctgtgggtga gccggtatcg
     1021 atatcggagg gccgggactg gcgctttctg gatacccggg gcatccgggt tgttcgtaat
     1081 ggtagtatgc tgtcgctccg ggcggaaacc accggggtct tccgctggca gtccgggaag
     1141 accaagggag tactggtttc caatatcgct cccagcgaac ttcagattgg gccaagaatt
     1201 ttagtaaccc aaaaatccaa agtccgcacg gcggcgggat atgaagtgaa ccggcaatct
     1261 ttggcagatt attttacctt gctcttcttg atactgctct gtttggaatg gatctgctgg
     1321 agcggcggtt ggcggatgat aaaggagtga atggcattgt ggtggaatag gccgatttgg
     1381 ctacttgcca tcctgttgat gattccgatt ttgtggccgg aactgttgtt gcggtggcgt
     1441 tttaaaaacc agagtcttga ggctttgtcc ggtcattctc cccgaagttc aaatggtctt
     1501 cgcaagcttt caaggttgcg ggccatcgca atatgcgccg caatactcag tctggccggt
     1561 acaggaatcc ttattcccac aaaaaatcgt caggtagtgg cgttagtcga tgtctccgcc
     1621 agtatcagtc cggctcaggt agaggtctcg cggggagcct tactggaact gctcggcagg
     1681 tttaaccccg gagatcgggt cgcggtaatt gcttttgcag gtgaaccgga gacgattgcc
     1741 tcctttgaaa ccccggcacg gctcagtatg atgttgaaga ccgctgccct taaagcaccc
     1801 cgaaccggag ccaccaatct ggaggcggca atacgtctgg gcagccggat gctttccgaa
     1861 aaaggcggca accgctgtat gatattgtta tccgatggcc ggcctactgt aggcggcatg
     1921 gaccttccgg caatggtcag ggagtcggat attcccattt atgtcttggg tgtgggaacc
     1981 tccggcagct cattgatcac ccgtgagcta agggtgccgg aggttatcca ccctcaggaa
     2041 cgggtattgg tccgttggga agtcgagagc gatcgcgttc aaaatgttat cgctacgatt
     2101 ttgatggata accggacggt gggccgggag ccggtaaaat tggccgccgg cagaaacacg
     2161 gtacttttgc cggttcaagt gaaggacccc ggaattcacc ggctggatat ccggattagc
     2221 gatactgccg ggaaagtctt gaatcaggct gattcggcca gtttactgaa tgtcaccggt
     2281 cctgcccggg tgcttgtgat tcatggcgac agtcccaatt cgccgattac gcgggcatta
     2341 caaactcagg gaatgcaact caccgaagag ggtgtggccg ggcttccgga gaatgccaac
     2401 ggactcgccg ggtacggggc tgtggttctg gataacattc cggcgcttta cctaagtcaa
     2461 ggccagcagg atgctttaca gggatatacg gccggcgggg gcggcctatt ggtggtcggg
     2521 ggcgacgcat ccctgggccg gggtgaatat tacgccaccg ggcttgagga tcttttgccg
     2581 gtgcagacgg ataccagaca acgcctattt ttcagccggg cgaatatcct gtttgtaatc
     2641 gaccactccg gatcgatgtc cgagatggtg ggtaaaacct cgaaacaaat ggctgcgatg
     2701 cagggtgtgg cagcggcgat cgaggaattg aacccccagg atcgggtggg aatcctcacc
     2761 ttcgatgttg agcccacatg ggtattgcgg tttaccccgg cggggcaaaa gaatcagatc
     2821 cggcgttcct tgagccggat tggccagggt ggtgggacgg atatgagtac tgccatggaa
     2881 gaagtggtca agggtttctc ctcggtcggc ccggtccgca gacacgtggt cattcttagt
     2941 gacgggcaga ctacgaatgc cgattttaac aagatctgcc gcaaactgaa gaatctcgga
     3001 gtcacgatta ccaccatcgg aatcggggaa gaggttaacg aaaccttgct aaaagatttg
     3061 gcacgctggg gagatggacg gtattaccgg gctcagctcg atcaaattcc tacggtgata
     3121 cggaaagaaa ccgtgcgggt gacccgggat cagattcagg aggggatgtt ctaccccaag
     3181 atcaaaactg ccgccccttt cctggaagta actgcctgga accggtttcc ggtcaagggc
     3241 tatttgatca ccaaacccaa gagtatggcc acagtttttt tagagatcgg gaagcaagat
     3301 cccttgctgg cctcgtggcg ctatggcaac ggcagggtgg cggtattcac cagtgattcc
     3361 ggcggtcgtt ggctttcttc ctggtcggga aggcctaatt tcaatcagct gtggagccaa
     3421 acgattcgtt ccattgagcg ggccgccatg gataccggat tgcgggtaac cgcccgggcc
     3481 gaagcagccg cggccgtaat cacggttgag gcggttggtg ccgatggccg gctccgtccg
     3541 ggacttcaat tgaccggcgc cgctctggat gatccgcctt ccggcgcaac aaatgcaggg
     3601 accgccaaca taaccgggga taatctcgtt tttacggaga ctgcccccgg tcattatgag
     3661 gcccgggtcc ccttatccgg atcggggtta aaacagttcc aggtccggga ggcgcaaagc
     3721 ggggaatggt ccataggatg gctttggaac ccgccgggac aggagaagct ttccaccggc
     3781 ccagatcgtt catttctggg caatatcgcc gccgcggcgg gaggttcctt attgaccgcc
     3841 gccaatccgg tcctgccgtt gccgggatgg gcctgggaaa aggttccttt gcaaaactgg
     3901 ctgattctcg cggctctggt atgtttcctg gtcgaactgt gtatccgtag tacttcgctg
     3961 ggacagatga caatggcccg ggcggttttc gcctcatggt gggaagcgca acgccgtatt
     4021 atcagtacga tccggggcag caggaccgaa caggaagagc gggaaaaaca gtcccgcgac
     4081 aatatatatg aagcgtaccg ctatttggcc gaaagggcac gccgccaaag ggaggaagat
     4141 caaaaaaatg cgcaaataca ttgagttcat actcctatct ttgct
//
LOCUS       DASVMB010000259         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_695453,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000259 DASVMB010000000
VERSION     DASVMB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695453"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF531_07580"
     CDS             <1..275
                     /locus_tag="VF531_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7713870.1"
                     /translation="DLTIEKNIGNYEMLVSNSAIGDGDDGKFVYVLNEKRGPLGNEYY
                     VWQVTVAIGESDNLKTAVLSGLQTGDKVIIGSDKQISDGSRVMVNQ"
     gene            293..1660
                     /locus_tag="VF531_07585"
     CDS             293..1660
                     /locus_tag="VF531_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713871.1"
                     /translation="MKPGRLGWIQLYTGILLIVMSIVLNGSLVSKITSMNGRYGFTKV
                     TASLKNQLDRSGQKGLILNEALWLEQFLNNGVITYRANPVTNLNVTFENQTCQAQILG
                     VNFRYPEFYQLKLLQGAFWNRSGDAGAQMVAVIDQELAWNLFKATRVVGRVLHLGNWP
                     VRIIGVIEQENSVFDWLTDSGVPKLYIPAATLLSIDNQARITNLEWLTADTGTLDLNK
                     TILSGALQQIGKNPDDFTLIDWNIKRVLLEQKPRLLTLVIGILIVFTLLRYLVQEFQT
                     VYFIVKVGCRSEYLRTVLKMKVQVIRGSAFKIILCLGAILLLWLGCAFKPYIPPRYIP
                     DELIDITYYVDLFQSGIQTGIAAMGDIPSHTEQLLHQAEMSTSWLLILGLAGGLPLLH
                     IGLSGVKRTGTGPYQIMLRCGLGVLGATLITAICAWLLGVPPVMDLRNIWIVWAFVFG
                     KALTS"
     gene            1917..>2455
                     /locus_tag="VF531_07590"
     CDS             1917..>2455
                     /locus_tag="VF531_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713872.1"
                     /translation="MVKKIFSLIMLGLIMVLGGTAFGQKGADKQAVILPEGAGYLEKE
                     IGRNSGVKIAGNVKLNSQNQLVVYDMGSGTPRYVILNTSGKPVGEIKAGFTGDGNVFA
                     LDAHDNLYVLAQNPITDAKKKTVAIDRTLYVYATTGSSKGKPIKQAALGRVMGDKAVL
                     INDLVVDTKGNLYCLTRTG"
BASE COUNT          641 a          519 c          659 g          636 t
ORIGIN      
        1 gggatctgac cattgagaaa aacatcggca attatgagat gctggtttcc aatagcgcca
       61 tcggcgacgg tgatgacggt aaattcgtat atgtactgaa tgagaaacgc ggccccctcg
      121 gaaatgaata ctacgtgtgg caagttactg tggctatagg tgaatcggat aatctaaaaa
      181 ccgcagttct gagcgggctg cagacagggg ataaggtcat catcgggagc gataaacaaa
      241 tcagcgacgg cagccgggta atggtgaacc agtgatgacg gaggcggttg ccgtgaagcc
      301 gggcagatta ggatggatcc aattatatac cggtatttta ttgatagtaa tgagtattgt
      361 tttgaacggc tctttggtga gtaaaatcac ctctatgaac ggccggtacg gctttaccaa
      421 ggttacggca agcttaaaaa accaactgga tcggtcgggg caaaaaggtt tgatcttaaa
      481 tgaagcgctg tggctggaac agtttttaaa taacggggtc attacatatc gtgccaaccc
      541 ggttaccaat cttaacgtta cattcgagaa tcagacctgc caggcgcaga ttctgggggt
      601 taacttccgc tatccggaat tttaccaact caaattgctc cagggcgctt tttggaaccg
      661 gtcgggtgat gccggggccc aaatggtggc ggttattgat caggagttag cctggaatct
      721 ctttaaagct acccgggtgg tcggccgggt attgcacctt ggcaattggc cggtacggat
      781 tatcggagtc atcgagcagg aaaattcggt ctttgattgg ctgaccgatt ccggggtacc
      841 gaagttgtac ataccggctg ctactttatt aagtatcgat aaccaggcac ggatcaccaa
      901 tctagagtgg cttaccgcag acaccggtac ccttgatctc aataaaacga ttctaagcgg
      961 agcgttgcaa caaatcggca agaaccccga tgattttacc ctgatcgact ggaacatcaa
     1021 acgggtatta ctggaacaaa aaccccgtct gttaacgctg gttatcggga tacttatcgt
     1081 attcacgctt ctacgttatc tggtgcaaga gtttcaaacc gtttatttta ttgtaaaggt
     1141 gggttgccgg agtgaatatt tacgtacggt tttaaagatg aaggtgcaag ttatccgggg
     1201 atctgcattt aaaataatcc tgtgtttggg cgccattctc ttattgtggc tggggtgtgc
     1261 ctttaaacca tatatccctc cccgttacat tccggatgag ctcatcgata tcacctacta
     1321 tgtagacttg ttccaaagtg gcatccaaac aggaatcgcc gctatggggg atataccttc
     1381 ccataccgaa cagcttctac atcaggcgga aatgtcgacc agctggttgt tgatcctggg
     1441 tttggcgggc ggattgcctt tattgcatat cggcctttcc ggagtgaagc gaaccgggac
     1501 cggtccgtat caaatcatgt tacgttgcgg cctgggggtg cttggcgcca cactcatcac
     1561 cgcgatctgc gcctggcttt tgggagttcc tccggtgatg gacctaagaa acatctggat
     1621 agtttgggct tttgtctttg gtaaagcttt gacttcctaa tatttgaggc aacagggaac
     1681 aggcaatagg caacagggaa gaggcgtgag gcatgaccct cggggcttta tcagttgtgg
     1741 acttcgctga gcccaatctt taagcactag attccagccg attcccttta aggggaaggg
     1801 tagataggcc ggaacttagg cctactacct attccaggat cggttgtcgt aaaagagtcc
     1861 ggttttggct ggggaagagt caatctttta agaaaaaaac gtaaaggaga atccgaatgg
     1921 taaaaaaaat tttcagtttg atcatgttgg gcctgatcat ggtacttggc ggtactgcct
     1981 tcggtcagaa aggggcggac aaacaagccg ttattctgcc ggagggtgct gggtatctgg
     2041 agaaggagat cggccgaaac tccggtgtca aaattgccgg gaacgttaaa ttgaactcgc
     2101 aaaaccagtt ggtcgtctat gatatgggga gcgggacccc gcgatacgtt attttaaata
     2161 cttcaggcaa accggttgga gagattaaag ccggttttac cggtgatggt aatgtttttg
     2221 ccctggatgc ccatgacaat ctatatgttt tggcgcaaaa tccaatcacg gatgcaaaga
     2281 aaaaaaccgt tgccattgac cggacgctct atgtctatgc aactaccggc tcaagcaagg
     2341 gaaaaccgat taaacaagca gcccttggca gagtaatggg tgataaagcg gtactgatca
     2401 atgatttggt tgtggatacc aaaggcaatc tttattgttt gacccggacc ggtat
//
LOCUS       DASVMB010000260         7551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_547657,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000260 DASVMB010000000
VERSION     DASVMB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547657"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..868)
                     /gene="cobS"
                     /locus_tag="VF531_07595"
     CDS             complement(125..868)
                     /gene="cobS"
                     /locus_tag="VF531_07595"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX7713873.1"
                     /translation="MIMIKRIRLAMGFLTVLPVNPRKIEPEDLGAAVKYFPIVGLLFA
                     GLVWAVLRLITPLGGKTLAGWLAAFAGAALNGWIHWDGWADTADGMGSRDPQKSLVIM
                     KDSRIGAFGTIALSFLILGKVITLSRLTTVPLGILIGVMTISRWAMVVLLATQPPVSQ
                     GLASTFRIERGRRDLIFASLWMLAVIVISDWRNIFLLGISLVLIPWVIRLSRKRFGGI
                     TGDLLGATNEMVELTCLIVLSLRFPWWNW"
     gene            complement(865..1431)
                     /gene="cobU"
                     /locus_tag="VF531_07600"
     CDS             complement(865..1431)
                     /gene="cobU"
                     /locus_tag="VF531_07600"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX7713874.1"
                     /translation="MSTMQSGASHILISGGVRSGKSRFAEGLALRLGGRVIYLATATA
                     GDAEMAERIARHQARRPQDWMTVEEPLHPEEVIRNYSKGYTILLDCLTLFLSNLYFEL
                     QPGAAESELSQLVLERFTELAQAVAVSRANLVLVTNEVGWGIVPDNPLARSFRDLSGL
                     ANQEIARVCDQVYLVVTGIPLRIKGTEG"
     gene            complement(1428..2480)
                     /gene="cobT"
                     /locus_tag="VF531_07605"
     CDS             complement(1428..2480)
                     /gene="cobT"
                     /locus_tag="VF531_07605"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX7713875.1"
                     /translation="MSMLEDTLYQIEPLNREVMAEVRKHLDQLTKPTGSLGVLEDIVA
                     QLGGITGQAFPDVSKKAVVVMAADHGVAIEGVSAYPQEVTAQMVQNFIRGGAAINVLA
                     RHTGATVVVVDVGVVGPVGSPQVRDCKIRPGTANIARGPAMSREEALRALEVGIELAS
                     ELSDQGYQLLATGEMGIGNTTPSSALTAVFLGISPAEVTGRGTGLSNAGLASKTAVIR
                     RTLEVNRPDPGDPLGTLASVGGLEIAALAGLILGAAAHRIPVVIDGFITTAAALVAAR
                     LKLQALDYVLASHCSAENAHRRLLDHLKLRPMIDLDMRLGEGTGAVLAFPLIEAATRL
                     LREMATFETAGISNRS"
     gene            complement(2510..3034)
                     /gene="cobO"
                     /locus_tag="VF531_07610"
     CDS             complement(2510..3034)
                     /gene="cobO"
                     /locus_tag="VF531_07610"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7713876.1"
                     /translation="MNQGIIQVYTGDGKGKTTAAVGLAVRAWGRGLRVKIFQFFKAPG
                     GSGEHWALPVFSPTIPIYPLGSGEFIINRGPNPEELAQAQRGWTQIEETLHDNAVDLL
                     VIDELSHAINNGLLKLETVLDTLRHRPKGIELVLTGRNMPAPILELADLVTEMKAVKH
                     PYLEGIEAREGIEY"
     gene            complement(3097..4146)
                     /locus_tag="VF531_07615"
     CDS             complement(3097..4146)
                     /locus_tag="VF531_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX7713877.1"
                     /translation="MRKEKAIIASTRKGLALGRKLASKYGCDIRIPESLTADEDETVY
                     FKETIPALLQEIVPVYTVLIMVMPLSTAVRSLAPLLCGQAKDTTVIVIDEEGRFVLNL
                     LAEDWEIANRLVQDLATQLGGTAVITTTSDLNNLPGLEAIARKYDLEIEQPELIPKYT
                     RGIGNGDPVVIWDQWGIEEYWPENIRIVRGAHPRLTEAEKMVLVIGYQEFPPVAPEVG
                     LLALRPVCLTVGINFEPGIAGVRITGAVRRYFRERHWSTRSIKTIAVPAGLEAEPGAR
                     EVCHDFGAVMAVFKIEDLQPVLPGWDPNAAQTDMESICEPVAILGAKQGKLIGPGMNL
                     GKVAVAVALGLNHTE"
     gene            complement(4267..4413)
                     /locus_tag="VF531_07620"
     CDS             complement(4267..4413)
                     /locus_tag="VF531_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713878.1"
                     /translation="MRVRTEFVTGDFPYEVGYLEISNYDDRKFLVTKVDPEHHFVWFG
                     IWDE"
     gene            4762..5343
                     /locus_tag="VF531_07625"
     CDS             4762..5343
                     /locus_tag="VF531_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7713879.1"
                     /translation="MKEHTPERAEALTLLKEYNQNDSLIRHALSVEAVMLHFAKLLGE
                     PDPDKWGIIGLVHDLDYEKYPEEHCQKVREILSANGWPEEYIHAVASHGWGICSDAKP
                     EARMEKVLYTIDELTGLVAAAALVRPSKSILDLELKSVKKKWKEKGFAAGVNRELITA
                     GAQELGMELDQVIGETIKGMQAVAEEIGLKGSL"
     gene            5439..5942
                     /locus_tag="VF531_07630"
     CDS             5439..5942
                     /locus_tag="VF531_07630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX7713880.1"
                     /translation="MKPALLVIDMQKQFFEGSEQTRRSLESAVEYINAAIDMFRKKNL
                     PVISIQHVNPEDGLTPEAPGFALPETLNVLPSDIHIHKTYGNAFTKTQLDAELRKLGV
                     DTLIITGFCAEYCVLSTYRGALDQDFHPILFRGGLASVNPENIGFVERVNDIISYGAL
                     NKVMENC"
     gene            6071..7522
                     /locus_tag="VF531_07635"
     CDS             6071..7522
                     /locus_tag="VF531_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7713881.1"
                     /translation="MDYIIQPGDNLFAIARRFGVSLEQLLAANPQITNPASIFPGQHI
                     TIPGPAPQVYLVQPGDTLSAIARRFGITVEQLLAANPQITDPNRIFPGQRINIPGAGP
                     QPGTTSYTIQPGDTLFLIARRFGTTVDNLLRLNPGIDPNRLLPGQQIRVPAPTPLLPP
                     GCIVYVSTRSGRPELWRSDAAGQGAVQITRESGTEAQPVANPKWSPDGRRIAYQAVGG
                     LFLIDPCGRSPILLAANVANYSWSHDSSRIAFSNAAGTFITDLAGNTRKVIDGFNNPV
                     WFPGDQRLAGSLPETEEIRFPHLATVDITGAGLTPVDTIPGRIVKLSPDGRFAVTEIF
                     SGSAFLVISSVLIYDFATQTRVDLPGFEIEVRPGIINNASFLGGWAPDSSRLVYSTII
                     NQNGLGEIRIANPRGTILQRFTRTYYPIPDWGPAPDWVIYTVSENPGTTVFDVAPPRN
                     IFVRNLRTSQEILIAGPADAYSPDWNGTTCTPC"
BASE COUNT         1835 a         2177 c         1834 g         1705 t
ORIGIN      
        1 ggacggagct gataataacc gtcatactcc ccaaagagca ctccgtcgat atcaataaat
       61 aaccgtttgg attctttgaa atccgtactc attgagatca tcacccctta tcattcccgg
      121 gactttacca attccaccag ggaaaccgca ggctcaacac tatcaggcaa gtaagttcca
      181 ccatctcgtt ggtggccccc aacagatcgc cggtgatccc gccgaaccgt ttgcgcgaca
      241 gccggatgac ccacggaata agtaccagag atattcctaa caaaaagata ttacgccagt
      301 cggagatgac gatcaccgct aacatccaaa ggcttgcgaa gatcagatcc ctccgtcccc
      361 gctcgatccg gaaagtactg gcaagtccct gagagactgg aggctgggta gccaagagta
      421 ccaccatcgc ccagcgggaa atagtcatga ccccaataag gatacccaac ggtactgtgg
      481 taagtctaga aagggtaatc accttgccca aaatcaggaa actcaacgct atggttccga
      541 aggctccgat acggctatct ttcatgatca ccaggctctt ttgaggatcg cggcttccca
      601 tgccgtcagc ggtatcggcc caaccgtccc agtgaatcca gccgttcaga gccgccccgg
      661 caaaagcggc caaccatccg gccaacgttt tgccgcccag cggagtaatg agacggagca
      721 ctgcccaaac taaaccggca aagagtaatc ctacaatcgg aaaatattta accgccgctc
      781 ccagatcttc cggttcaatc ttacgggggt tcaccggaag cacagtcaga aatcccatag
      841 ccaaccggat tcgtttaatc ataatcatcc ttcagtcccc tttatccgca ggggaattcc
      901 ggtaactacc agatacacct gatcacaaac ccgggcgatc tcttggttgg ccaaaccgga
      961 gaggtcccga aaggaccggg ccagaggatt atcgggcaca attccccagc ccacctcatt
     1021 ggtgaccagc accaggttgg cccggctgac ggcgacagct tgagcgagtt cggtgaaacg
     1081 ttcgaggact aactgagaga gctccgattc ggcggcgccg ggctgcaact cgaaatagag
     1141 attgctcaaa aacagtgtga gacaatccag gagaatcgta taacccttcg agtagttccg
     1201 gatcacttct tcaggatgca aaggctcctc tacagtcatc cagtcctgtg gccggcgggc
     1261 ctggtggcgg gcgatccgtt ccgccatctc cgcatccccg gcggttgcag tcgccaggta
     1321 gattactctg cctcccaaac ggagggcgag gccctcggca aaccggctct tcccggaacg
     1381 gacaccaccg ctaatcaaga tgtgactggc tccagattgc attgtactca tgatcggttc
     1441 gaaatccctg cggtttcaaa agtagccatt tcccgaagca gtctggtagc tgcctcgatt
     1501 aatggaaatg ccaataccgc cccggttccc tctcccagtc tcatatccag atcaatcatc
     1561 ggacggagct tcaaatgatc aagcaaacga cggtgagcat tctccgccga acaatgtgac
     1621 gccagcacat aatcaagggc ctgcagcttg agcctggcgg ccactagagc cgctgcagta
     1681 gtaatgaacc catcgatcac tacgggtatc cggtgggctg ccgcgcctaa aatcaacccg
     1741 gcgagcgccg caatctccaa accgccgact gaagcaaggg ttcccagcgg atcgcctgga
     1801 tccggtcggt taacttcaag ggtgcggcgg attacagcgg tcttagatgc caatccggca
     1861 tttgaaagcc cggtgcctct ccctgtcacc tcggccggtg agatcccaag aaacacggct
     1921 gtcaaggcgc tggatggggt ggtattgccg atgcccatct cccctgtggc cagcagttga
     1981 taaccttgat ccgataattc cgacgccagc tctatgccaa cttccaaggc ccgcaacgcc
     2041 tcttcgcggc tcatggccgg acctcgtgct atattggccg taccgggacg aatcttacag
     2101 tcgcgaactt gaggtgaacc caccgggcct accactccca cgtccactac cacgactgtt
     2161 gccccggtat gacgggccag tacgttgatg gcagcgccgc cgcggatgaa attttgaacc
     2221 atctgagccg ttacttcctg aggataagca ctgactccct cgattgccac tccgtgatcg
     2281 gcagccatca ctactaccgc ttttttggag acatccggaa acgcttgccc ggttatcccc
     2341 cctagctgag caacgatatc ctccaacacc cccaagctcc cggtgggttt ggtaagctga
     2401 tccagatgtt tgcggacctc cgccatgacc tcccggttga gcggctcaat ctgatataac
     2461 gtatcttcca gcatagacat aacgcaacct ccgatgtatc ggtgagtact taatactcaa
     2521 tcccctcacg ggcctcgata ccttccagat aaggatgttt gactgctttc atttccgtaa
     2581 ccagatccgc caactctaga attggtgccg gcatgttccg gccggtaagc accaactcga
     2641 tccctttagg gcggtgacgc aaggtgtcga gcactgtctc cagtttcagg agaccattgt
     2701 taatagcatg acttaattca tcgatcacca gcaggtccac tgcattatca tgaagggttt
     2761 cctcgatctg agtccagccc cgctgggctt gagccaactc ctccggattt ggtccccggt
     2821 tgatgatgaa ctccccggaa ccgaggggat aaattgggat agtagggcta aaaaccggta
     2881 aggcccaatg ttcaccggaa ccgcctgggg ctttaaaaaa ctggaagatt tttacccgta
     2941 agccgcggcc ccaggcccga accgccagac cgacagcagc cgtggtcttg cccttcccgt
     3001 ctccggtata aacttgaatg attccttgat tcatatacag ccaccctcat ctctattcag
     3061 tcgcgtacta tgatctattc agccggatac tgccatttat tcggtatgat ttaatcccag
     3121 ggccaccgcc actgctacct ttcccagatt catcccggga ccgattaatt ttccctgttt
     3181 ggccccaagg atcgccaccg gttcgcagat ggactccata tccgtttgag ctgcatttgg
     3241 gtcccaaccg ggcagcaccg gctgcaaatc ctctatttta aaaactgcca ttacggcccc
     3301 aaaatcatgg caaacctcac gagcacccgg ttcggcctct aggcccgccg gaacagcgat
     3361 ggtcttaata ctccgggtcg accaatgacg ctcccgaaaa tagcggcgga ctgcgccggt
     3421 aatccggact cctgcaatgc ccggctcaaa attaatccct accgtcaaac ataccggacg
     3481 gagtgccaac agtccgacct ccggagcgac cggcggaaat tcctgataac cgatgactaa
     3541 caccattttc tctgcttccg tcagacgggg atgagccccc ctgacgatcc ggatattctc
     3601 cggccaatac tcctcgatcc cccactgatc ccaaatcaca accggatccc cgttgccaat
     3661 cccccgggta tacttcggta tgagctccgg ttgttcaatc tcgagatcat attttcgggc
     3721 gattgcttcg agccccggca gattattcaa gtccgaggtc gttgtgatca ccgcggtacc
     3781 tcccaattgc gtggccaggt cttgtactag ccggttggca atctcccaat cctcggccag
     3841 cagattgagg acaaaacgtc cctcctcatc aatgacgatg accgtggtgt ctttcgcttg
     3901 gccgcacagt aacggtgcca aactgcgtac cgcggtgctc agaggcatta ccataatcaa
     3961 aacggtataa acaggaacga tctcttgtag caacgcaggg atggtttcct tgaaatatac
     4021 cgtttcatcc tcatcggccg tcagggactc cgggattcgt atatcacatc cgtactttga
     4081 tgccagtttc cggcccagcg ccagtccctt ccgggttgac gcgatgatgg ctttctcctt
     4141 tctcatgccc agtatccctc ggttttatgt ttcaaataaa aaaacccgcc gcaagcgggg
     4201 tttgattcac caattgtcaa aatgccggaa ccatttccgc caaggctttt ctgaattgcc
     4261 gtccaatcat tcatcccata taccgaacca aacgaaatga tgctcggggt ctaccttggt
     4321 gactagaaac ttgcgatcat catagttact gatttcgagg taaccgactt cataggggaa
     4381 atcgccggtt acaaattcgg tccgtactcg catcttcatc ccctcggagt cctgctcgat
     4441 aaattccagg taaggtaagt aggtgagaga cttgtacaat tgaaagtcaa gggactgaga
     4501 accttcaggg tcttctttgg ggttaacggg ttctatctcg tagcgcgttg tggacccccc
     4561 cttcttctaa ctaattatac cagtatcgcg taaatctggc tacaccttca aattactttt
     4621 atgaaacaac cagcccattt gtaacgattt ctgtaatcta actatcccat taagactatc
     4681 agttatattg catattttga taaataacgg taatattaag acaaaaatat acaaaggcac
     4741 catccgaaag aaggcctgtt gatgaaggaa catacccctg agcgggcgga agccctgacg
     4801 ctgttgaaag agtataatca aaatgatagc ctgattcgtc atgccctatc cgtggaagcg
     4861 gtaatgttgc attttgccaa gctgttaggc gaaccggacc ctgacaaatg gggcattatc
     4921 ggtctcgtcc atgacctcga ttacgaaaaa tatcccgagg agcattgcca aaaggtccgg
     4981 gagattctct ccgccaacgg atggccggag gaatacatcc acgccgtggc cagccatggc
     5041 tgggggatct gttccgatgc caaaccggaa gcccggatgg agaaggtgct ctacaccatt
     5101 gatgaattga cgggactggt ggctgcggct gcgctagtcc ggcccagcaa gagtattctt
     5161 gatttggagc tgaagtcggt caaaaaaaag tggaaagaga aggggtttgc cgcgggagtc
     5221 aaccgggaac tgatcacagc cggtgcgcag gaattgggca tggaacttga tcaagtgatt
     5281 ggcgagacca tcaagggcat gcaagccgtg gccgaggaga ttggtttaaa aggaagcctt
     5341 taatctattt cgtaatatta cccggggatc gaaataaaaa ttgaagtgaa tatattttaa
     5401 tactgtgcca aaaacagtat gaaagggagt gttttgtgat gaaaccggct ctactcgtca
     5461 tcgatatgca aaagcagttt ttcgaaggct ccgagcaaac ccgtcgttca ctggaaagcg
     5521 ccgtggaata catcaacgcc gccattgata tgttcaggaa gaagaacctc ccggtcatca
     5581 gcattcaaca tgtcaacccg gaggatggct tgactccgga agctccggga ttcgcgcttc
     5641 ctgaaacgtt aaatgttctt ccctccgata ttcatattca taaaacctac ggcaacgctt
     5701 tcaccaaaac tcaactcgat gctgaattaa ggaagcttgg agtcgacacc ttgatcatca
     5761 ccggtttttg tgccgagtat tgcgtgctct ccacttaccg gggcgcgctc gatcaagatt
     5821 tccatccgat ccttttccgg ggcgggctag ccagcgtcaa tccggaaaat atcggctttg
     5881 tagagcgtgt caacgacatc atcagttacg gtgcgttaaa caaagtgatg gagaactgtt
     5941 aaattatccc gaatcgatat ggcgcgttac gcgctgaaca cctccaatct gattggcctc
     6001 tgcgatgcca gtggcaccac aggtgccaac ggcataatct atagtgacca tagattgcga
     6061 ggtgttttgg atggactaca tcattcaacc tggagataat ttattcgcta tcgcccggcg
     6121 ctttggggtg agtttggaac aattactggc ggccaacccg cagattacca acccggcttc
     6181 aatattcccc gggcaacaca tcaccattcc cgggccggcg ccccaggtct acctcgttca
     6241 gcccggcgat acattatccg ccattgccag acgttttgga atcacagtgg aacaattgct
     6301 agccgccaac ccgcagatca cagaccccaa ccggatcttc ccggggcagc gtatcaatat
     6361 tccgggagcc gggccgcagc ccggaaccac cagttacacg atccagccgg gagacaccct
     6421 atttctgatc gcccggcgtt tcgggaccac ggttgataat ctattacgcc taaatcccgg
     6481 tatcgatccg aaccggctcc tcccggggca gcaaatccgg gtgcccgcac caacaccgct
     6541 actccccccc ggatgcatcg tctatgtttc aacccggagc ggtcgaccgg aactatggcg
     6601 cagtgacgca gccggacaag gagctgttca aatcacacgg gagtccggca cagaggcaca
     6661 gcctgtagcc aaccccaaat ggtcgccgga tggtcggcgc attgcttacc aagccgttgg
     6721 cggtttattc ctgatcgatc cttgcggccg ctccccaatc ctgctggccg cgaatgtagc
     6781 caactattcc tggtcgcacg acagcagtag aatcgctttt tccaatgccg caggaacatt
     6841 catcaccgat ctcgcaggga atacccggaa ggtaatcgac ggtttcaata atccggtctg
     6901 gttccccggc gatcaaaggc tggccggttc cctgccggaa acggaggaga tccgtttccc
     6961 tcatttggcc acagtcgata taaccggggc gggcttaacc ccggttgata ccataccggg
     7021 ccggatcgtc aaattgtccc ccgacggcag gtttgccgtg accgaaatct ttagcggatc
     7081 cgctttttta gttatttctt cggttttaat ctacgatttt gccacccaaa cccgggtgga
     7141 tctgcccgga tttgaaattg aagtgcggcc cggcatcatc aataatgcca gtttcctggg
     7201 aggctgggcg ccggattcgt cccggctggt atatagcacc atcataaatc aaaacggcct
     7261 tggcgaaatc aggatcgcca atccccgggg aacgatcctg caacgattca cccggacgta
     7321 ttatccgatt cccgattggg ggccggcacc ggactgggtg atttataccg tctccgaaaa
     7381 tccggggacc accgtttttg atgtcgctcc accccgaaac atttttgtac gcaatctccg
     7441 caccagccaa gaaattctaa ttgccggacc ggcggatgca tattctccgg attggaacgg
     7501 gactacctgc acgccttgtt gaaagagcaa atggacccat tctttctgga a
//
LOCUS       DASVMB010000261         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_621751,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000261 DASVMB010000000
VERSION     DASVMB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621751"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..693
                     /locus_tag="VF531_07640"
     CDS             34..693
                     /locus_tag="VF531_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713882.1"
                     /translation="MKFRIISLIMVFLVLAGSYGYASGRSLIYQSVTKGVAVTINEKI
                     EINGAETIVTSSSIDQGTNRVRNRDNSALVWELVNDRTDTRLTAERVGDIIKLEGKFE
                     GKTVDKTLKIDGKPWFQAWDLSLGSFVLSGKKSLEFWTVRNDLKAFRMAAFRDKEETL
                     EINGRNVEAVKVKVSLPGWMAKFWSASYWFRKSDGVFVKYQGANGPPGTPETVIELVN
                     E"
     gene            712..2241
                     /locus_tag="VF531_07645"
     CDS             712..2241
                     /locus_tag="VF531_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7713883.1"
                     /translation="MEKSIIIIGAGIAGLSAGCYGQMNGYRTRIFEQHNQPGGLCTAW
                     KRKGYTFDGCISWLVGSAPGTGVYPIWEELGAVQNRQFVNHDEFFRIEGEGGKTLIVY
                     TSIDRFAKHLKELAPNDTAIIDELAQAVRVMSKFGSPVGKAGELYNWFDYLKLIPKMA
                     PFGGIFKKYGSMTVRKFAEGFSDPFLRKAFGSFFSIPDFPMIAFLSTLGWMHRQAAGY
                     PIGGSLEFSRAIEGRYLDLGGEIHYQSRVVKILTEENRAVGVRLADGSEHRADRVVSA
                     ADGHATIYEMLEGKYIDAEIERRYREMPIFQPLIQVSLGVNRDLGKEPHQITYQLKQP
                     ISIAGKLREEIGVRHYCYDPTMAPAGKSVLVVMFDSNYQYWKELGADRGKYRAEKESI
                     AELTVRGLEQRFPGLAGQIEVIDVATPLTFERYTGNWQGSFEGWLITIQTFNLKIKKT
                     LPGLDDFYMIGQWVQPGGGLPPEAQAGRDLIQVLCKKDRKWFGSNKPKAVPALGAKPA
                     V"
     gene            2502..3170
                     /locus_tag="VF531_07650"
     CDS             2502..3170
                     /locus_tag="VF531_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713884.1"
                     /translation="MTVVRVRKLQKNYAVGGGAFRALQGIDLDVADGEFLALVGPSGS
                     GKTTLLNIIGCLDSPSDGEVWLGERQVTELTPNQLADLRRDRLGFIFQSYNLIPVLTA
                     FENVEYPLVLKKVKPKERAEAVKAILAEVGLGEKLDSFPHQLSGGQQQRVAIARALVT
                     RPMLVLADEPTANLDSHTGREIISLMQQLNREKGVTFIFATHDQRVMEHASRRIELVD
                     GRIA"
     gene            3274..>3755
                     /locus_tag="VF531_07655"
     CDS             3274..>3755
                     /locus_tag="VF531_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713885.1"
                     /translation="MAAFFRMAARNLLRHKRRSVLTGLIIGLGALMAVLAFSFSHSVG
                     AGMIDSAIRSCTGNIQIHAPTGEQIEILNPSDEIPLLMDTDRITSMIVKNPMVAAVAP
                     RLRFGGLLYKGTDEPNGVMLVGIDPIREPRITPKIKLIRGSYLTEPDGILLGQSMAKA
                     "
BASE COUNT          910 a          851 c         1073 g          921 t
ORIGIN      
        1 tatattcatg catcctgaaa cggagggtat cccatgaagt ttcggattat ttcactcatc
       61 atggtctttt tggttctggc cggatcatac ggatatgcaa gtggaagaag tctaatctat
      121 caaagcgtca caaaaggcgt agcggttaca atcaatgaga agattgaaat taacggcgcc
      181 gaaaccatcg ttacctccag ttcaattgat caagggacga accgggtgag gaatcgcgat
      241 aattcggcat tggtttggga actcgtgaat gaccgtacgg atacccggtt aacggccgag
      301 cgggtcgggg atatcatcaa gcttgaggga aaattcgagg gaaaaacggt tgacaaaacg
      361 cttaagattg acggtaaacc atggtttcaa gcatgggatc tgtcgctagg atcattcgtt
      421 ctctccggta aaaaatcctt ggaattttgg acggtccgca atgatttgaa ggctttcagg
      481 atggctgctt tcagggataa agaagagacg ctggagatca atggccggaa tgtggaagcg
      541 gtcaaggtaa aggtttcctt acccggctgg atggcgaaat tttggagtgc ctcctactgg
      601 ttccgaaaat cggatggggt ttttgtaaaa tatcaaggtg caaacgggcc tccgggcact
      661 ccggagacgg ttattgaatt ggttaacgag taattgaagg agggttagca tatggaaaaa
      721 tcgatcatta tcattggcgc aggaatcgcc ggactttccg cgggttgtta tggtcagatg
      781 aacgggtatc gcactcgcat ctttgagcag cataatcaac cgggtgggct ttgtacagcc
      841 tggaaacgca aggggtatac cttcgacggc tgtatctcct ggctggtcgg gtcggcgccg
      901 ggaaccggcg tttatccgat ctgggaggaa ctgggggcag ttcaaaatcg tcaattcgtt
      961 aatcatgacg agttcttccg gattgagggt gaaggtggaa aaacgttgat tgtttatacc
     1021 agcatcgatc gatttgcaaa acacctcaag gaactcgctc ccaatgacac cgccatcatt
     1081 gacgagttgg cccaggccgt ccgggtgatg tcaaagttcg gctcaccggt tggtaaagcc
     1141 ggggagctgt ataactggtt cgactatctt aaacttattc ccaagatggc tccgttcggg
     1201 ggaatcttta agaaatacgg ttcgatgaca gtccggaagt ttgcggaggg tttcagtgat
     1261 cccttcctgc gcaaagcatt cggaagtttc tttagcatac cggattttcc gatgattgcc
     1321 tttctgagca ccttgggctg gatgcaccgc caggcggcgg gttaccccat cggcggttcc
     1381 ctcgagtttt cccgggccat tgagggacgc tacctggatc tgggcggtga aatccattat
     1441 caatcacggg ttgtcaagat tttaaccgag gagaaccggg cggtcggagt ccggttggct
     1501 gacgggagcg aacaccgcgc cgatcgggtt gtttcagcgg cggacggtca tgccaccatt
     1561 tacgaaatgt tggaaggcaa gtacatcgat gccgagattg aacggcgtta ccgggagatg
     1621 cctatctttc agccactcat ccaggtctcg ctgggcgtaa accgcgatct gggtaaagaa
     1681 ccgcatcaaa ttacctatca actgaaacaa cctatctcta tcgcgggaaa gctccgtgag
     1741 gaaattggcg tccgccatta ttgctatgac ccgaccatgg ctcctgccgg aaaatcggtg
     1801 ctggtggtga tgtttgactc caactaccag tattggaagg aactcggcgc cgaccgcggc
     1861 aaataccgcg ccgaaaaaga aagcatcgcc gagttgaccg tcagagggct tgagcaacgt
     1921 tttccgggcc tcgcaggtca gattgaggtg atcgatgtcg ccacccccct taccttcgaa
     1981 cggtataccg gcaattggca gggctccttt gaaggttggc tgattactat tcaaaccttc
     2041 aatctgaaga tcaagaagac cctgccggga ctggatgatt tctatatgat tggccaatgg
     2101 gtccaaccgg gaggcggttt gccccctgaa gctcaggccg gccgggatct gatccaggtg
     2161 ctctgcaaaa aggatcggaa atggtttggg agcaataaac ctaaagcagt tccggctctt
     2221 ggagcaaaac cggcagtttg aattacgtta ctggagaggc tttttagatt aactttgtta
     2281 tatgacaatt cctgttttta agactagatt tcactgttgt cattaagcaa ctcacccccg
     2341 tcccctctct ttttgaaaag agaggggtgg ttggatgtaa ttaatattga tgggtgtgcg
     2401 tcggggtgag ttcctatcag tttcggccat caaccatcat ttcagacaga gtcgtatatc
     2461 cgatattggc cggctccatt tcttcgggga gggatttaca tatgactgta gtgagggtgc
     2521 gaaaactcca aaagaattat gcggtgggcg gaggggcctt ccgggcattg caggggatcg
     2581 atctggatgt tgccgacggc gaatttttgg ctttggtcgg gccttccggg tcgggcaaga
     2641 ccacattact caatatcatc ggctgtttgg acagtcccag cgatggcgaa gtatggctgg
     2701 gtgaacggca ggtaactgag ctaaccccca atcagttggc ggatctgcgc agggaccggt
     2761 tagggttcat ctttcagtca tataacctta tcccggtgtt aacagccttt gagaacgtag
     2821 aatacccgtt ggttttaaaa aaggtaaaac caaaggagcg ggctgaagcg gtcaaggcaa
     2881 tcctggcgga agtggggttg ggggaaaaac tggatagttt tccgcatcaa ttaagcggcg
     2941 gtcaacaaca acgggtagca atcgcccggg cgctcgttac ccggccgatg ttggtgctgg
     3001 cggacgaacc taccgccaac ctggattccc ataccggtcg ggagatcatc tccctgatgc
     3061 agcagttgaa ccgggagaag ggagtcacct tcatcttcgc cactcatgat cagcgggtca
     3121 tggaacatgc ctcgcgacgg atcgaactgg tggacggtag aatcgcctga caaggatcat
     3181 ggcactcctg ctattgttcc gagggttagc gcgtatcaat ggagtgcgta aacacactgc
     3241 catgtatgcg cgatgatgaa gggagttact caaatggcgg cttttttcag aatggcagct
     3301 cgtaatcttc tgcgccataa gcggcgtagt gttttaacag gtttaatcat cggccttggg
     3361 gccttgatgg cggttttagc cttttcattc agccattcgg taggggctgg gatgattgac
     3421 tcggccatca gatcctgtac cggtaatatt cagattcatg caccaaccgg ggaacaaata
     3481 gagattctca atccgtcgga tgagattccg ttacttatgg atacggaccg gattacatcg
     3541 atgatcgtta aaaatcccat ggtagccgcg gtagccccgc ggcttcgttt cggggggttg
     3601 ttgtataaag ggacggatga gcccaatggg gtgatgctgg tggggatcga cccaatacgg
     3661 gaaccgagaa tcacgcccaa aatcaaattg atccggggct cttacttaac cgaaccggat
     3721 gggattttgc tcggccagag catggcgaag gcctt
//
LOCUS       DASVMB010000262         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_108225,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000262 DASVMB010000000
VERSION     DASVMB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108225"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VF531_07660"
     CDS             <1..744
                     /locus_tag="VF531_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7713886.1"
                     /translation="LSHMQEMLTALFFSPTEFMDYQPNQFKIGEELRLECKYLEEIKQ
                     HFSSCNKTELLKTVEKLMTEIQKVHLGIHYIQEISYKVYYIIQNELAVSNNQNQDQEI
                     LARPEWVDYPYFLSFPKWREMLLSFIQDSLVFIERRCKMVNLGISREVIEYVHLHYKE
                     PINLKQIADKFFVNAVYLGRAFQKATGVNFKQYVNHIRIAEAKRLLLHTNKLIYEIAN
                     EVGYTESKYFIVKFSQEVGKSPTEYRNRV"
     gene            complement(797..1492)
                     /locus_tag="VF531_07665"
     CDS             complement(797..1492)
                     /locus_tag="VF531_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX7713887.1"
                     /translation="MKDKMDIRCKPAGPLNLYTHFIGANPVTSFNPKSIFPSIDVTAF
                     IGPFSTVIGDVTIKENVFIAPSVSVRADEGSPFFIGPNTNLQDGVILHGLNEGRVTVN
                     GKKYSIYIGGNVSCTHGCIIHGPCKLGNWVFIGFNAIVFNAIVEDGSYISTDAIVTGG
                     VKLAPNRFVPTGAIIDTQSKADALRPVPSDRQEFAKEVQHINSEFPLAYSLMFGSIRC
                     SCGLACEPGSLVN"
     gene            1682..2107
                     /gene="umuD"
                     /locus_tag="VF531_07670"
     CDS             1682..2107
                     /gene="umuD"
                     /locus_tag="VF531_07670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEX7713888.1"
                     /translation="MSINIYSSDQASTLPLQYVGRQISAGFPSPADDYLETVLDLNKL
                     LIKNPSSTFYGRVKGFSMKDAGVDNGDLLVIDKSLPYRNDALAVCYVDGEFTLKRIKV
                     EGGRIILMPANKDYQPIVINEAADFAVWGIVTYIIKKVY"
     gene            2108..>2776
                     /locus_tag="VF531_07675"
     CDS             2108..>2776
                     /locus_tag="VF531_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEX7713889.1"
                     /translation="MFALVDCNNFYVSCERVFNPSLNGHPVVVLSNNDGCVIARSGEA
                     KTLGIQMGAPAFKIAGFLEANHVAVHSSNYALYGDLSHRVMNTLGRFTPELEIYSIDE
                     AFLNLAGLSVEFMEYAQRIRRTVLKNVGIPVSVGMGPTKVLAKTANHYAKRIPENEGI
                     CVLDSREKINAALKKFEIDEVWGIGRRYTNLLKSLNIKTAWDFIQMPDRWVKKKMTVV
                     GLRLK"
BASE COUNT          819 a          608 c          617 g          732 t
ORIGIN      
        1 ttgagtcata tgcaagaaat gttaacggcg ctgtttttct ccccgactga atttatggat
       61 tatcaaccga atcaatttaa aattggcgag gaattacgtc ttgaatgcaa atacctggag
      121 gaaataaagc agcatttttc atcatgtaat aagaccgagc tgttgaagac tgttgaaaag
      181 ctaatgactg aaatacaaaa agtacatctg ggaattcatt atatccaaga gataagttac
      241 aaggtatatt acatcatcca aaacgagtta gccgtatcca ataatcaaaa tcaagatcaa
      301 gaaattctgg cccgtcctga atgggtggat tacccctact ttctttcatt tccgaaatgg
      361 agagaaatgt tactctcttt catacaggat agtttagtct ttattgaacg cagatgtaaa
      421 atggttaatc taggtatcag cagagaagtt atcgagtacg tacacctgca ctataaagaa
      481 ccaatcaacc taaaacaaat tgcagataag ttttttgtga atgctgttta tttaggcaga
      541 gcctttcaaa aggctaccgg agtgaatttc aaacagtacg tcaatcatat ccgaattgcc
      601 gaagcaaaaa ggcttctgtt gcacaccaac aagctgattt atgaaatcgc gaacgaggtt
      661 ggctataccg agagcaaata cttcattgtc aagttcagtc aggaagtggg caaaagtccc
      721 accgaatatc gaaaccgggt gtagttgata tatttactca ttttaaggta ttataatttg
      781 atttcaaacc gatattctaa ttcactaaac tgcccggttc acaagccaac ccgcaactgc
      841 aacggattga tccgaacatt aatgagtagg ctaaaggaaa ctcgctgttg atatgctgaa
      901 cttcttttgc aaattcttgc cgatcagacg gtaccggtct caaagcatcc gctttgctct
      961 gagtatcaat gatcgctccg gtggggacaa aacggttcgg cgcgagtttc acaccgccgg
     1021 ttacaatggc atcagtcgat atgtaagagc catcctcaac gatcgcatta aagacaattg
     1081 cgttaaaacc aataaagacc caatttccta atttacatgg cccgtgaata atgcagccat
     1141 gagtgcagga aacattgccg ccgatataaa ttgagtattt cttaccattc acggttaccc
     1201 tgccttcgtt taagccatgc aaaattacac catcctgaag attcgtattc ggcccgataa
     1261 agaacggact accttcatca gcgcggacag agacactggg agcgataaat acattttctt
     1321 taattgtcac atcaccgatt accgtggaaa aaggtccgat aaaagctgtg acgtcaatgg
     1381 acggaaagat gcttttgggg ttaaatgatg tcacagggtt ggcgccgatg aaatgagtat
     1441 ataaattgag cggacctgcc ggtttacacc ggatatccat tttatccttc atcagtaaca
     1501 ctccattcaa cctatctcaa ttattcaatt gatattatat gaatggcaaa tcgaagtggt
     1561 atcggagatt tatatggtaa ttccctagaa ccacctaaaa cttccagttc ttttttcgat
     1621 ggaatctgct ataataaaaa tatatcgaac atatgtttgg atatagagga gattccacat
     1681 catgagcatc aacatttatt cttcggatca ggcttcgaca ctcccactgc aatacgtagg
     1741 ccgtcaaata tcggccgggt ttccctcccc ggccgatgat tatctggaaa ccgtacttga
     1801 tctcaataaa ctgctgatca aaaaccccag ctcgactttt tacggccggg tcaaagggtt
     1861 ttccatgaaa gacgccgggg tagataacgg tgatctgtta gtcatcgata agtccctgcc
     1921 ctatcggaac gatgccctgg cagtctgtta tgtggatggc gaatttactt taaaaagaat
     1981 aaaggtcgag ggcggcagga tcatcctgat gcccgccaat aaggattatc aacccattgt
     2041 cattaacgag gccgccgatt tcgcggtctg gggaatcgtg acttatatca tcaagaaggt
     2101 gtactgaatg tttgcgctgg tggattgtaa caacttttac gtctcctgcg aacgggtgtt
     2161 taatccttcg ctaaacggcc accccgtggt ggtgctttcc aacaacgacg gctgcgtgat
     2221 tgcccgctcc ggcgaagcta aaacgcttgg catccagatg ggagcaccgg cattcaaaat
     2281 cgccggtttc cttgaggcaa accatgtggc ggtgcactct tcaaactacg ccttatacgg
     2341 cgatttatcc caccgggtca tgaacaccct gggaagattc accccggaac ttgagatcta
     2401 ttcgatcgat gaagcttttc tgaacctggc cggtttgtcc gtagagttca tggagtatgc
     2461 ccaaaggatc cgccggaccg ttttgaaaaa cgtcgggatc ccggtcagcg tggggatggg
     2521 tcccaccaaa gtgctggcca aaaccgccaa ccattacgcc aagaggattc cggagaatga
     2581 agggatttgt gtccttgaca gccgggaaaa aatcaacgcg gcactgaaga agtttgagat
     2641 tgacgaggta tgggggattg gccggcgtta cacgaaccta ttgaaatccc taaatatcaa
     2701 gacggcctgg gatttcattc aaatgcctga tcgctgggta aagaagaaaa tgactgttgt
     2761 cgggctgcgg ttaaaa
//
LOCUS       DASVMB010000263         8389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_32784,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000263 DASVMB010000000
VERSION     DASVMB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32784"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1758)
                     /gene="aspS"
                     /locus_tag="VF531_07680"
     CDS             complement(31..1758)
                     /gene="aspS"
                     /locus_tag="VF531_07680"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7713890.1"
                     /translation="MLRTHHCNELRQEQIGIKATLAGWVDTIRDHGSVKFLDLRDQHG
                     VTQIVIHDDSVLKGVNRETVIAATGTVRARDIENINPKLDTGMLELHAESIRVLGPCC
                     NVLPFEIRDSVATREDVRLKYRFLDLRNPAVHQNIVLRSKVINFLRRRMEELGFMEIQ
                     TPILTASSPEGARDYLVPSRKFKGKFYALPQAPQQFKQMLMVSGFDKYFQIAPCFRDE
                     DARGDRSPGEFYQLDLEMAFAEQEDVLNVAETVLYDAFTKFSDKRVSSAPFRRITYHD
                     AVLKYGSDKPDLRNPLTICDLSEFFTHVEFAAFKNKPVRGIVANCAGRPRSFFDNSVN
                     YAMSIGMKGLGYLTLAADDFKGPIAKFLSPDQKKQLISACDMKEGQTVFLISDSQDII
                     NKLAGQIREWLGENLGIIDKEAFEFCFVIDFPMYEHNPETHKTDFTHNPFSMPQGGLN
                     ALETKDPLEILAYQYDVVCNGVELSSGAVRNHSPEIMKKAFQIAGYSEEELEKRFGAL
                     YTAFQYGAPPHAGMAPGIDRIVMLLAGEEAIRDVIAFPLNGNAQDLLMGAPCEVTEHQ
                     LREANIKIR"
     gene            complement(2135..2761)
                     /locus_tag="VF531_07685"
     CDS             complement(2135..2761)
                     /locus_tag="VF531_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7713891.1"
                     /translation="MDIYEAIKLRRTVRDFKDEKIEITMIKKILNAGLLAPTNDHMRS
                     WNFVIMNDPAAKLELIKLVVKDRTEKQAITTLDNWGLVDRSQREMYIDAIPKQYKMLL
                     NCGCLIVPCFQQTEPLLEPSKLSSLNGFASIWCCIENILLSAVAEGIYGVTRIPGDDE
                     LTHIKTILNIPDDYEVPCYLALGYPAENLKELRQNFVNVEERMHFNQW"
     gene            3331..4890
                     /locus_tag="VF531_07690"
     CDS             3331..4890
                     /locus_tag="VF531_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7713892.1"
                     /translation="MSSKIPSPDAPEERLSPDLDRNLAWIRDALCQSPDLVIRNFIIR
                     IHTNIDRGWEAAVAYIDGLADNTVINDHILRPLMGDTIKLSANQVINPEKNLLWIEDH
                     LITANDLSRITTLNETIEAIIKGDTVLLVQNSAKAIQISSKGWEKRAISEPQSEVIIK
                     GPRDGFIENLRTNTALLRRKIGDPHLTFESMRIGRQTRTDLNLAYIRGIARDDLVAEV
                     RRRIRRIDTDAILSGSNIMEFIEDAPFSLFPTTAYTERPDVAAAKLLEGRVAIMVDGT
                     PTVYTVPALFVESFQSPDDYHFRPYYITLIRWIRFLAFAFTVFLPAFYVALTTFHQEL
                     IPTPLLITIAAATDTTPFPVVIETLGTGILFEVLREAGLRIPRAFGPAVSIVGGLVIG
                     EAAVGAGLVGEPMVVVTALTAIASYVVPFQAEVNVFLRLGLTIIAGFFGLYGIFNGTI
                     LILIHLASLRSFGIPFLSPLAPLMASDLKDVIIRAPQWALLTRPRSLEPKESGRQKFG
                     SGPKHPGKNDE"
     gene            4880..6088
                     /locus_tag="VF531_07695"
     CDS             4880..6088
                     /locus_tag="VF531_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7713893.1"
                     /translation="MMNKKKNRNVPQLIFSLVLVFGLFCCSGCWDRKEIKTQGIVAGM
                     GVDWNTDGSGKYLHTFQVIKPGEVTHPKSKGNGGGDEPPVWVGTSEGKTPYESGRNIA
                     FQSSRRLFFAHNQTLIFGKQLAQKGVRPPLDLELRFSQIHSLELIMIAKGTAKEVLEV
                     PGGLAKIPAFDISGMVGDAKYTSEAIPVTLEDFFGYLVSKTRAPVAPQIEVFTAGKEK
                     RLRLAGTAVFRKDRWIGELDKTETRGLLWVLGQVKRGVINIPDQRGIEVAAAEIVTTE
                     RKLRPEIRHGKVVIRIQINTEGNLQDILSPQGLTDPGKMNDKLHELEKLEAAVIRQEI
                     LAAWRKARRLNADVFGFGEALHRRHPRQWKSMEPEWDRLFPRIGLKVEVNTALRRVGE
                     IMTPAIPLPK"
     gene            6176..7282
                     /locus_tag="VF531_07700"
     CDS             6176..7282
                     /locus_tag="VF531_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HEX7713894.1"
                     /translation="MPVQEQGKISASQLLLMVASFTMGSSTLFPPGGGIGQDNWIAIL
                     LGLAEGIFFALIYLALANRFPGKTLVDIADLVWGPYLGKLVSAIFLLYLFHLGSLALT
                     VMMNFVKFTFLPVTPVSVFVLFGVLVCVAAASAGVEVLARSVTILVIFVTVIFFLIDG
                     MLLPQIKLENLQPVLETPLLKLLAAGHRAATFPFGDSVAFLMLIPFLNAPRKTRSSVT
                     NGLIFAGLMLTIANVRNIGVLGATAEYFLYPSYSATRLINVGEVFTRIEILIGIIFLT
                     TNFIKITILIYGFMLGTAQLCKLKSFRTLTIPVWILISLLAIHNYANVVESQEFVRMV
                     YPFYSLPFQVGIPLITWIAALIRKQPREGVPRKP"
     gene            7331..7540
                     /locus_tag="VF531_07705"
     CDS             7331..7540
                     /locus_tag="VF531_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713895.1"
                     /translation="MKAFLVMIAFVLLYFFRLPGLLPEEYRRDLVVFTFFMIIAFIIS
                     MLAMIGVKFPYPSTEITKIFKAVFH"
     gene            7784..>8389
                     /locus_tag="VF531_07710"
     CDS             7784..>8389
                     /locus_tag="VF531_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7713896.1"
                     /translation="MFQFFKRKLTFLKKSRQHDSTDQATVPASPKISLAEISQTLSKN
                     FELIHEIFDNSADLIVRRFTIGALPTEAMVVNLNGMSELKLINDNIIGSLMWETRKAG
                     LAERITLEYLKDSAINLNAICETGSMDEAVTAILNGDTALFLDGSAKALIMNTRTWAH
                     RGVQQPETEPVVRGSREGFTETLSINISQIRRKIKDPELYFE"
BASE COUNT         2093 a         2113 c         1957 g         2226 t
ORIGIN      
        1 ggtctttgat ggggggagca ctttcctgtc ctagcgtatt ttgatatttg cctcgcgcag
       61 ctggtgctcc gtgacttcac aaggcgctcc catcaggaga tcctgcgcgt ttccgtttaa
      121 cgggaacgcg atgacatcac ggatcgcctc ctcaccggcc agcagcatca cgatacggtc
      181 gatgcccggc gccattccgg catgaggggg cgctccgtac tggaatgccg tatataatgc
      241 accaaacctc ttctccaact cctcttcgga atatccggca atctgaaacg cctttttcat
      301 gatctccggc gagtggttcc ggactgcgcc cgacgagagc tcaacaccgt tgcagacgac
      361 atcgtactga tacgccaata tttccagggg gtccttggtc tccagggcat tcaagccccc
      421 ctgcggcatc gaaaaggggt tatgggtaaa atcagtcttg tgggtctcgg gattgtgctc
      481 gtacatcgga aaatcaatga caaagcagaa ctcaaacgct tccttgtcga taatcccgag
      541 gttttcccca agccactccc gaatctgccc agccagctta ttgattatat cctgactgtc
      601 gctgattaag aagactgtct ggccttcttt catatcgcaa gcgctaatca gctgtttttt
      661 ctgatccgga gacagaaatt tagcaatcgg acccttaaaa tcatccgcgg cgagggtgag
      721 gtaaccgagc cccttcattc cgatgctcat ggcgtaattg acggaattat caaaaaagct
      781 ccgcggacgg cctgcacagt tggccacaat cccgcgtacc ggcttattct taaaggccgc
      841 gaactcaaca tgggtgaaaa actcactcag gtcacagatg gtcagcggat tgcgcaaatc
      901 cggtttgtcg ctgccgtatt tcaggaccgc gtcatgatag gtaatacgcc gaaaaggtgc
      961 ggaggatacg cgcttatcgg agaatttagt aaacgcgtcg tataaaacgg tttcggcgac
     1021 gtttaggaca tcctcctgct ccgcaaaggc catctccaaa tcaagctgat aaaattcacc
     1081 cggtgaacgg tcgccccggg catcttcatc ccggaagcag ggggcaatct ggaaatactt
     1141 gtcgaagccg gagaccatca gcatctgctt aaactgctgc ggcgcctggg gcagggcgta
     1201 gaatttgcct ttgaacttac ggctgggaac caggtaatcc cgggcgcctt ccggcgagga
     1261 cgccgtcagg atcggcgttt ggatttccat aaatcccagt tcctccatgc gtcttctcaa
     1321 gaagttgata accttcgaac gcagaacgat gttttggtgg actgccgggt ttctcaggtc
     1381 cagaaagcgg tatttcaaac ggacatcctc gcgtgtggca acagaatccc ggatctcaaa
     1441 cggcaataca ttgcagcatg gacccaaaac cctgatgctt tcggcatgca gttcgagcat
     1501 gccggtatcc agtttgggat tgatgttttc aatatccctc gcccggacgg ttccggtcgc
     1561 cgcgatgacg gtttcgcggt tgaccccctt aagcacggag tcgtcatgaa tcacgatctg
     1621 cgtcacgccg tgctggtcac gcaggtccaa aaacttcacg ctgccgtgat cccggatcgt
     1681 atcgacccaa ccggccagag tagccttgat tccgatctgc tcctgcctta gttcattgca
     1741 gtgatgtgtc cttagcatcg attcatcctc caaaaataaa aatcccggaa attatctcta
     1801 aataatctcc gggacgagca tttttgtttt gcccgcggtg ccacccgcat tgatgcggga
     1861 aacccgcatc cactttctta ttcaatttcc ggtatagtgt accacatcta ttgatgaagt
     1921 gttccccggc ttactactct tccaatcgcg ctctcaatcg gtgacgcgaa tccctgttgg
     1981 aatttccaag aaagcggagt cttcatgatg aattatatgt tatgtcgaaa taactgtcaa
     2041 cagatatgaa gggtaaagag taaagggtaa aagagaaaaa tgtggtcatt cgtttctgca
     2101 ttttatttca tgagaacctg aagtaggcgc gctgttacca ttgattaaaa tgcatccttt
     2161 cttctacatt tacaaaattc tgccttagtt ccttcaaatt ttctgccgga tatcctaacg
     2221 ccaaataaca gggaacttcg taatcatctg gaatattcaa gatggttttg atatgtgtta
     2281 attcatcatc ccccgggatt ctggtgacgc catatattcc ctctgctaca gcagataata
     2341 aaatattttc gatacaacac cagatggagg cgaatccatt taaggatgat aatttactcg
     2401 gctctaaaag cggctctgtc tgctgaaagc aggggacgat caaacaccca caatttaaaa
     2461 gcattttata ctgcttggga atcgcatcaa tatacatttc acgctggctt cggtcaacaa
     2521 ggccccagtt atccaaagta gtaatggctt gcttttcggt tcgatccttt acaaccaatt
     2581 taataagttc caatttggct gcaggatcat tcatgatgac aaaattccat gatctcatat
     2641 gatcattggt cggtgccaat aatcccgcat tgagtatttt tttgatcatc gtaatctcaa
     2701 ttttttcatc tttaaaatcc cttaccgtcc tcctcaattt gattgcttca taaatgtcca
     2761 aagtgatctc ctcctccatg tttcattcaa agttattggg tatttatagc ctatcctgtt
     2821 ttttcattcc gagctccccc ggaaggaaca aagccgttct actccaagaa tgatagggat
     2881 tttaccacct cgacagtttt ccgaactaat gttcgatata taatattatt ataattcgaa
     2941 caaacgtttg ctgtcaagcg aattttcatt agctagtcaa aaaaatcatt ccataattca
     3001 ttagcagaga catctcttgt attcatctga tctcaggacc ggccaaacaa atgggagggg
     3061 ttttttaaga aatatggttc ccggggccat ccgccaattt gcatcttata cataggcgca
     3121 ttcgcgattt ttatatggat gacccttcct tataagttat ctccaagcat tattgctaaa
     3181 atcatcacct ctctgttcca aatgaaaaag ttaattcaaa gccataatag cttatgatat
     3241 tccaacttga tcaatatttt acgatatggg gaatttaaag gtaatctaat gtttagatgg
     3301 ttattaaaaa agtataaagc aattgcgaat atgagttcca agattccgtc tcccgacgct
     3361 cccgaagaac gtctctcccc ggaccttgac cggaatcttg cctggatccg tgatgcccta
     3421 tgtcaaagtc cggatctggt gatccgcaat ttcattattc gcattcatac taacatcgat
     3481 cggggttggg aggcagccgt tgcctatatc gacgggttag ccgataatac cgtcatcaac
     3541 gatcatattc tgcgtccgct gatgggagat acgatcaaat taagcgccaa tcaagtcata
     3601 aatccggaaa agaatctgct ttggatcgag gatcatctca tcaccgccaa tgatctctcc
     3661 aggattacaa ctttgaacga gacaatcgag gccataatca aaggcgatac cgtcttgctc
     3721 gtccagaatt ccgccaaggc tatccagatt agctccaagg gttgggaaaa aagggctata
     3781 agcgagccac aatcggaagt catcattaag gggccccgcg atggctttat tgaaaatctc
     3841 cggaccaata ccgcgctttt acgccggaag atcggggacc cccatctgac tttcgagagc
     3901 atgcgcattg gccgccaaac ccggaccgat ctcaacttgg cctatattcg gggtattgcc
     3961 cgggatgatc tcgtggcgga agttcgccgc cggatccggc ggattgacac cgatgccatt
     4021 ctgtccgggt ccaatattat ggaatttatc gaagatgccc ccttttcact ctttccgacc
     4081 acagcctata ccgagcggcc cgatgtagcg gcagccaaac tgctggaagg gcgggtggcg
     4141 attatggtgg acggcactcc gacggtttat accgtgcccg cgctctttgt ggaaagcttt
     4201 caaagtccgg acgattatca ttttcgtccg tattatatta ccttgattcg ctggatccgt
     4261 tttctagcct ttgcctttac cgtattctta cccgccttct atgtagccct tactaccttc
     4321 catcaagagt taatccccac cccgcttctc atcaccattg cggcagcgac ggatacgact
     4381 cctttcccag tggtgattga aaccttggga acgggaattc tttttgaagt tttacgtgaa
     4441 gccgggctaa ggattccccg tgcctttggc ccggcggtca gcattgtagg cggtctggtg
     4501 attggcgagg cagccgttgg tgccggactg gtcggggaac cgatggtcgt tgtcaccgct
     4561 ttaacggcca ttgcatcgta tgtcgtgcct tttcaagcgg aagtcaatgt cttcttgcgg
     4621 ttaggcttaa caattatcgc gggttttttc ggcctctacg ggattttcaa cggcacgatt
     4681 ttaatcctga ttcacctggc gtccctgcgc tctttcggca taccattctt atcgccgctg
     4741 gccccgctga tggccagtga tctcaaagac gttatcatcc gtgcgcccca atgggcgctg
     4801 ttgacccggc cgcgttccct ggaaccaaaa gagtcgggcc ggcaaaaatt tggctcaggc
     4861 cccaaacacc cgggtaaaaa tgatgaataa gaagaaaaat cgcaatgtcc cgcaactcat
     4921 attttctttg gtattggttt ttggcctgtt ttgttgcagc ggttgttggg accggaaaga
     4981 aataaaaacc caaggcattg tggcgggaat gggggttgac tggaacaccg acggcagcgg
     5041 caagtacctt cacacatttc aagtgataaa acccggtgaa gtgacccacc ccaaaagcaa
     5101 gggcaacggg ggaggcgatg aaccaccggt ctgggttggc accagtgaag gaaagactcc
     5161 ctatgaatcc ggccggaata ttgcctttca aagcagccgc aggttgtttt tcgcccacaa
     5221 ccagacatta atttttggaa agcaattggc ccaaaaagga gtccggcccc cgctagatct
     5281 tgagttgcgt ttctcccaga ttcattcttt agaattaatc atgattgcta aggggacagc
     5341 gaaagaggta ttggaagtcc cgggcggttt ggcaaagatc cccgcctttg atatctccgg
     5401 aatggtagga gacgctaaat acacctccga agcaatcccg gttaccctgg aagatttctt
     5461 cggttacctg gtcagcaaga cccgggcacc ggtcgctccc cagattgagg ttttcacggc
     5521 cggcaaggag aaacggcttc gtctcgccgg tactgcagtt ttcagaaagg atcgctggat
     5581 tggcgaactg gataaaaccg agacccgcgg cctgctgtgg gtgcttggtc aagtcaagcg
     5641 cggggtaatc aatatccccg accaacgcgg catcgaagtt gcagcagccg aaatcgtcac
     5701 caccgaaaga aaactccggc cggagatccg ccatggtaaa gtcgtgatcc ggattcaaat
     5761 caataccgag ggaaacctgc aagatatttt aagcccccaa ggattgacag accccggcaa
     5821 aatgaatgat aaactgcatg agctggaaaa actggaagcc gccgtaatcc ggcaagagat
     5881 cctggcagcc tggagaaaag cgcgccgtct gaatgccgat gtctttggct tcggcgaagc
     5941 gctgcaccgc cgacaccccc gtcagtggaa atccatggaa cccgaatggg accggctctt
     6001 cccccggatt gggctcaagg tcgaggtgaa taccgccctc agacgggtcg gagaaatcat
     6061 gacaccggca ataccattac cgaaataatc aagtaatatc aatacccgtt ccgtaaacgg
     6121 aaacatgttg gacggcggta ctcccaggaa tcattccttc gaaatgagga aagccatgcc
     6181 ggttcaagaa caaggcaaaa tatccgcctc gcaattattg ttgatggttg ctagctttac
     6241 gatgggttcc tctaccctct ttccaccggg gggcggcata ggccaggata actggatcgc
     6301 gattctctta gggttggccg aagggatatt ctttgccttg atctatcttg ccctggcaaa
     6361 tcgctttcct ggtaaaaccc tggtcgatat cgccgatctg gtctggggtc cttaccttgg
     6421 caaactggtc tccgccattt ttcttttgta tctcttccac cttgggtcac tggcgctcac
     6481 cgtgatgatg aattttgtca aattcacctt tcttccggtc acgccggttt ccgtatttgt
     6541 tttattcgga gttttggttt gcgtggcggc cgcttcagcc ggcgtcgagg ttctggcgcg
     6601 ttccgtgacg atcctggtta tattcgtgac tgtcatattt ttcctcatcg acgggatgct
     6661 gctgccgcag atcaaattgg aaaatctcca gccggtcctg gaaacgccct tgctcaaact
     6721 tctcgccgcg ggccacaggg cggctacgtt tccttttggc gatagcgtgg cttttttgat
     6781 gctcatccct tttctcaatg ccccgcgaaa aacccgctcc tccgtcacga acggccttat
     6841 cttcgccggt ctaatgctga cgatagctaa tgtccggaat atcggcgttt tgggcgccac
     6901 ggcggagtat tttttatatc ccagttatag tgctacccgc ttaattaacg tgggggaggt
     6961 ttttacccgg attgaaatac tcatcgggat catctttttg acgactaatt ttattaaaat
     7021 cacaattttg atctatggct tcatgttggg tacggctcaa ttatgtaaac tcaagtcttt
     7081 tcgaacgctg accattccgg tctggatctt aatctcgctg cttgcgattc acaattatgc
     7141 caatgtggtc gaaagccagg aatttgtgag aatggtttat cccttttact ccttaccgtt
     7201 tcaagtcggt attcctctta tcacttggat tgcggcgctt atccggaaac agccgcggga
     7261 aggggttcca cgaaaaccat aaccacaggc ttaaccggtt cattctttta tttgtcccta
     7321 tggaggtaaa atgaaagctt ttttggtgat gatcgccttt gttttgcttt atttcttccg
     7381 gttgcccggc ttgttgccgg aagaataccg gcgggatttg gtcgttttta cattcttcat
     7441 gatcatcgcg tttatcatca gtatgctggc gatgattgga gttaagtttc cctatcccag
     7501 cacggaaatc acgaagatat ttaaggctgt atttcattaa agtgtaaatg gtaaagggaa
     7561 atcacgaacc atctggccaa aacctgaagg caccatcatg tgtacaattg caaaggaatt
     7621 cacgcaatta acccattgct gaagcgtaaa tggctaacgg atggatacca acggagtact
     7681 ttgtaaataa tagtttcagg attcgtaatc gagggatttt atatcacttt gaaagcatat
     7741 ccccttattc taaatctacc tgccggcttc cctaaggaga cccatgtttc aattctttaa
     7801 gcgtaagttg acgtttttaa aaaaaagcag acaacatgat tccactgatc aagcaacagt
     7861 acccgcgagc cccaaaatct ctctggcaga aatatcccaa actctctcga aaaattttga
     7921 actcattcat gaaatcttcg ataacagcgc tgatttgatc gtccgtcgct tcaccattgg
     7981 agctctcccc accgaagcca tggtagtgaa tttgaacggg atgagtgaac taaaattaat
     8041 caacgacaat atcatcggtt cgttgatgtg ggaaacccga aaagccgggc tggcagagcg
     8101 gatcaccctg gaatacctga aagattcggc cattaatcta aatgcaattt gcgaaacagg
     8161 ctccatggac gaagcggtaa ccgccattct aaacggcgat accgcccttt ttctggatgg
     8221 ttcggccaaa gcgttgatca tgaataccag gacctgggcc caccggggtg tccaacaacc
     8281 ggagacagaa ccggtggtta gaggttccag ggaaggtttc acggaaacgc tatccattaa
     8341 catcagccaa atccggagaa aaatcaaaga tcctgagtta tatttcgaa
//
LOCUS       DASVMB010000264         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_254531,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000264 DASVMB010000000
VERSION     DASVMB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254531"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VF531_07715"
     CDS             complement(<1..1037)
                     /locus_tag="VF531_07715"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEX7713897.1"
                     /translation="MKDRTLALTPPMGWNSFNTFGCEPDEQLVKEIADTMVSSGLKDA
                     GYEYINIDDGWMATERDDQGNLFPRPEKFPHGLKHLTDYIHSLGLKAGIYLGCGLKTY
                     GEKPGSLGYEEKDAALIGAYGFDLLKYDRRCLPEDPPGRDSIAEYTRMSKALMSTGRS
                     ILFSLCEHGNSAPWSWGAEVGHMWRTTSDIKDCFEGEYTWGWSFNKIIDQTCDLYPYA
                     GPGHWNDPDMLVVGLHGIPEWMGPGCTDVEYRSHFSLWCLVAAPLLIGCDIRKMSAVT
                     KSILTNPEMIAVNQDKLGKQGHRITVGEQMQVWIKPLEKGAYAVGLYNRSDSPKTIIV
                     DWHDLGLETDI"
     gene            complement(1220..2167)
                     /locus_tag="VF531_07720"
     CDS             complement(1220..2167)
                     /locus_tag="VF531_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713898.1"
                     /translation="MAELLKVENLKKYFPRGNRVLKAVDDVSFTIKPGETLGLVGESG
                     SGKSTLGRAILRLIEPDSGKVVFDGENLSHLHKEMLRVKRRNMQIVFQDPLASLNPQM
                     TIGQAIEDPLLVHGIGNAAERSDMVIDLLDMVGINRRFINSFPYEFSGGQQQRVGIAR
                     ALALSPKFIVCDEPVSALDVSIQAQIIALLVDLRKRFDLSYLFISHDLGVIKYISDRI
                     AVMYLGKIVQIGPKDSVFANPAHPYTRAIISSVPKIPRDGKQMRHYETLKGEIPSPIN
                     LPPGCRFKSRCPVARPECAESEPKLKEISAGHWVACHLC"
     gene            complement(2160..3140)
                     /locus_tag="VF531_07725"
     CDS             complement(2160..3140)
                     /locus_tag="VF531_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713899.1"
                     /translation="MAPLLSVNNLRTYFYRSEGVVRAVDGISYTLDHGESLGIVGESG
                     SGKSVSVLSLLGLLRKNGRVEDGEAIFDGRDLLKLNKEDLREVRGKEISVIFQDPMTS
                     LNPVFRVGPQMMEPPLWHRLFGNKESKQRAMQLLKRVGIPEHESRFSDYPFQFSGGMR
                     QRVMIAMALTCEPKLIIADEPTTALDVTVRSQILNLLQDMKDEFGMSIIMITHDFSIA
                     CNFCDNIIVMYAGKIMESASTKEFLANCLHPYSQGLLRSTLDIDTQGIKLNPIPGNPP
                     NLLDPPSGCSFHPRCERRQSICSAKVPALRQVRPGHSLSCHFAEGGRQNG"
     gene            complement(3142..4062)
                     /locus_tag="VF531_07730"
     CDS             complement(3142..4062)
                     /locus_tag="VF531_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7713900.1"
                     /translation="MQNKTMSVTVNNQPQTTPGLQKGADRSISYWEAAWRRFKQNKMA
                     VIGGVIVLVLVVIAILTPLIAPYRYDEAHYDHTFESSSVRFIFGTDDLGRDMFSRILY
                     SLQNALMVAFGAQLVTLVLGILLGAIAGFRGGAVDALIMRFVDIVFAFPTFLFNVILV
                     TVLGRGLFTIFLAIGLVNWAGLARLVRGQILSLKQAEYVEAARALGAKDSHIILRYLL
                     PNTMGIIIVSFMTGIPAAMMTESGLSLLGMGLRPPMPSFGNLLNTGTGMILGFPHLLV
                     WPALIFAVILLGFTYLGNGLRDALNPRSDA"
BASE COUNT         1067 a         1140 c          997 g          941 t
ORIGIN      
        1 ttgatatcag tctctaaccc cagatcatgc cagtcgacga tgatggtttt cggggaatcg
       61 ctccggttat aaagccctac cgcgtaggcc cctttttcca agggtttaat ccagacctgc
      121 atctgctcgc caaccgtgat ccggtggcct tgtttgccca gtttatcctg attgaccgcg
      181 atcatctccg ggttagtcag gatactcttg gtcacagcgc tcattttccg gatatcacag
      241 ccgatcaata gcggtgccgc taccaggcac cagagactga aatgggaacg gtactccaca
      301 tcggtacaac cgggtcccat ccattccggg atgccgtgta acccgaccac cagcatgtcc
      361 gggtcgttcc agtgccccgg accggcatag ggatagaggt cacaggtctg atcgatgatc
      421 ttgttaaaac tccatcccca ggtgtactcg ccctcaaaac aatccttaat atccgaagtg
      481 gtacgccaca tatgtccgac ttccgccccc caagaccagg gagcggagtt tccgtgctca
      541 caaagactga aaagtatcga ccggccggta ctcattaagg ccttgctcat tcgggtatac
      601 tcggcgatac tgtcacggcc gggaggatct tccggcaggc aacggcgatc gtatttcaac
      661 aggtcaaatc cataggcccc aatcaatgcc gcatctttct cctcatatcc gaggctcccc
      721 ggcttctcac catatgtctt cagcccgcaa cccagataaa tcccggcctt tagccccagg
      781 gaatgaatgt aatcggtcag gtgcttcaac ccgtggggaa acttctcggg ccttgggaac
      841 aggttgccct gatcatcccg ttccgtagcc atccaaccgt catcgatatt gatatattca
      901 taccccgcat ccttcaaacc gctggatacc atggtatcgg cgatctcttt caccaactgt
      961 tcatcgggtt cgcagccaaa agtgttgaag gagttccaac ccatcggcgg cgtgagcgca
     1021 agggtccgat ctttcatggt gtttcctccc taaaatttta tcttatccga aaccaattcc
     1081 aatactgtct aaaagcaatt cacccccttg gtcgcgcgtc tttcagacgc gacctctcat
     1141 gaacgctttc aagagaggag gacggctcag gagtaataat aagtaacatc gatctcaggg
     1201 ggtgagttgc ggatatttat taacagaggt ggcaagccac ccaatgtccg gcggagatct
     1261 ctttgagttt cggttcggat tcggcgcact ccggccgggc caccgggcaa cgggatttga
     1321 aacggcaacc ggggggaaga ttgatcggtg acggaatctc ccccttgaga gtctcataat
     1381 gccgcatttg tttaccatcc cgggggatct tgggtacgga agagataatc gcccgggtat
     1441 aggggtgggc cggattggca aaaaccgaat ccttcggccc gatttgaacg atcttgccta
     1501 aatacatcac cgcgatccgg tcggagatat acttgatgac ccccagatcg tgcgagatga
     1561 aaagatagga aagatcgaac cgcttgcgga gatccacaag caacgcgatg atctgggcct
     1621 gaatcgatac gtccaatgcc gagaccggct catcgcagac gataaattta gggctcaaag
     1681 ccaaagcccg ggcaattccc acccgttgct gctgccctcc ggaaaactcg tacggaaagg
     1741 agttgataaa ccgccgattg atacccacca tgtcgagaag atcaatcacc atatcggatc
     1801 tctcggcagc attgccgatt ccatgaacca ggaggggatc ttcaattgcc tgtccgatgg
     1861 tcatctgggg attgagcgaa gccagcggat cctgaaagac gatctgcata ttacgccgct
     1921 tgacccgtaa catttcctta tggagatgag ataaattctc tccgtcgaag acgactttac
     1981 cggagtccgg ctcgattaaa cgcaagatcg cccgtcccag ggtagatttg ccggaaccgc
     2041 tctcaccgac gagtcccagt gtttcaccgg gtttaatcgt aaacgagacg tcatccacgg
     2101 ccttcagcac ccggttgcct ctggggaagt atttttttag attttccacc ttcaataatt
     2161 cagccatttt gtctaccccc ttccgcaaaa tgacaggaaa gcgagtggcc cggtcttacc
     2221 tggcgcaagg cgggaacttt ggccgaacag atcgactggc ggcgttcgca gcgcggatgg
     2281 aaactgcaac ccgagggcgg atccaaaaga ttgggcggat ttccgggaat cgggttgagc
     2341 ttgataccct gggtgtcaat atccaacgtc gaccggagca gaccctggga gtatggatgg
     2401 agacaatttg ccaaaaactc cttggtcgat gcggactcca tgatcttccc ggcatacatc
     2461 acgatgatgt tatcgcaaaa attacaggcg atgctgaagt catgcgtaat catgatgata
     2521 ctcattccga actcatcctt catatcctga agcagattga gaatctgtga acgtaccgtc
     2581 acatccaggg cggtagttgg ttcatcggcg atgatcaatt tcggctcaca ggttagggcc
     2641 atggcgatca tcacccgttg ccgcattcca ccggagaact gaaacggata atcactaaat
     2701 ctcgactcgt gctccgggat gcctaccctt ttcagaagtt gcatcgccct ttgtttcgat
     2761 tccttatttc caaacagcct atgccaaagc gggggttcca tcatctgagg gccgaccctg
     2821 aagaccggat tcagactagt catcggatcc tgaaagatta cactgatctc cttaccacgg
     2881 acctcgcgga gatcctcttt gtttagtttt aataaatcgc ggccgtcaaa gattgcctct
     2941 ccatcttcaa cccggccatt ctttcgtaac agtcctaaaa gggacagtac cgatacggat
     3001 ttacccgatc cgctctcgcc gacaatgcct aaactctcgc catgatcgag ggtgtaacta
     3061 atgccgtcga cagccctaac gacaccttcc gagcggtaaa aataagtacg aagattatta
     3121 acgctgagta atggagccat atcacgcatc actccttgga tttagtgcat cgcgcagacc
     3181 attacccaaa tatgtaaaac cgagtaatat cacggcaaaa atcagggccg gccagacgag
     3241 caaatgcgga aaacccaaaa tcataccggt cccggtgtta agcaaattac cgaaactggg
     3301 cattggcggc cgtaagccca tcccaagcag tgaaagcccg ctctcagtca tcatcgccgc
     3361 gggaatgccc gtcatgaaac tgacaataat tatccccatg gtattcggaa gtagataacg
     3421 aaggatgata tgactgtcct tggcgccgag agcccgggcc gcctcgacat actccgcttg
     3481 cttgagggag aggatttggc cacgcactag ccttgccaat cccgcccagt taaccaaacc
     3541 gatagctaaa aagatggtga aaaggcctct gcctaaaact gtgaccagga tgacgttaaa
     3601 gagaaatgtc ggaaaagcga acacaatatc cacgaaccgc atgatcagcg catctacggc
     3661 cccgccccgg aaaccggcga tggcgccgag caggatgccg agaacgagag tgaccaattg
     3721 agcaccgaaa gctaccatca acgcattctg cagactgtaa agaatgcggc taaacatatc
     3781 gcggcccaga tcgtccgtgc caaaaataaa acgaacactg gaactctcaa aagtatgatc
     3841 ataatgagct tcgtcatagc gatagggcgc aatgagaggc gttagaatcg cgataacaac
     3901 taataccagg acaataactc caccaatcac agccatttta ttctgtttaa aacgccgcca
     3961 ggccgcttcc cagtaactga tactgcgatc cgcacctttt tgtagacccg gtgtggtctg
     4021 cggttgatta ttaacagtaa cgctcatcgt cttattttgc acctcattca acctcctact
     4081 gcagtttgat ccggggatcc agtattgagt agatgatatc aaccaatagg ttgatgatca
     4141 tgacc
//
LOCUS       DASVMB010000265         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_32890,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000265 DASVMB010000000
VERSION     DASVMB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32890"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF531_07735"
     CDS             <1..618
                     /locus_tag="VF531_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7713901.1"
                     /translation="FLYNTLEIIRMEADSKGVRNVGQMIYLIATLFRNYTKSGTIITI
                     GDEIELCRLYLQLFNLKYQNKLTFTARIPAEIINYSIIKFSLQPIIENCLVHGIDRDI
                     PGSVITLEGFKEQNEIHILIRDNGAGISIDKLNALKEALKEGDHYPSSTIGLSNVNER
                     LRMVYGRNYGLSIESEAKAGTTVELVIPALKKEDLREYVQGVLGG"
     gene            593..2185
                     /locus_tag="VF531_07740"
     CDS             593..2185
                     /locus_tag="VF531_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713902.1"
                     /translation="MYKVFLVDDEPFILKGLYTIIDWNEYGLEIAGQATNGLEALEQL
                     EKKPADIVIADITMPKMDGLELIRRLKRINPTTKFIILSGYNEFDFVKTGIELGIENY
                     LLKPVNVQELGATLTSTVQKLEDSAGKMPLTKKDFNILRDNILYRWVTHNIDPLELKE
                     RSPFLQIGLEYSDYTVAIINVIREETEDDASSRFNSEEKLGQTYNRCLAMIDGTVAGV
                     CFCDPQGESIFIFGESAGLLNQSNIDVKLAQLQEDLKQTLKVDTFITIGDSQKSFYNV
                     YQSYEHAKKLQEYLLVYSSGKTLRFDDLKTLPADGDASPAIDYETFSKLLLSKSQGEV
                     FAFIDQVFETLLKREGIQPSTIQNCAVEIVLHIYETLSNLEIGCNPATNDYREFFFFI
                     FQFRTLGQLKNYLKNIAASAINRFSSEDQKKSPVIKQVLNYINLHYQDELSLKMLGSM
                     FNVNSTYLGQLFQKETGYLFSDYVNRFKIEKARQMLLNTNLRAIEISKKSGFSDPNYF
                     FKQFKKYVGVSPTELRNFKKSE"
     gene            complement(2190..>2469)
                     /locus_tag="VF531_07745"
     CDS             complement(2190..>2469)
                     /locus_tag="VF531_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX7713903.1"
                     /translation="CRIVDDMGIPVIAYGLRSDFRGNLFPGSEALLALADSIEELKTI
                     CWCGKKAIMNTRLLNGKAVYEGEQVQIGGNESYISLCRKHWREGKVKP"
BASE COUNT          720 a          587 c          552 g          610 t
ORIGIN      
        1 tttttatata acacgctgga gattatcaga atggaggccg attccaaagg cgtccggaat
       61 gtgggacaaa tgatttacct catcgccacc ttattccgga attatacgaa aagcgggaca
      121 atcatcacta tcggggatga gatcgagtta tgccgccttt accttcagct tttcaacctc
      181 aaatatcaaa acaagctgac ttttaccgct agaattccgg ctgaaatcat caattacagt
      241 atcatcaaat tctcactcca gccaatcatc gaaaactgcc tggtgcatgg gattgaccgg
      301 gatattccgg gcagcgttat taccctcgaa ggttttaagg agcaaaatga gattcatatc
      361 ctcatcagag ataatggggc cggaattagc attgataagc tgaatgcctt aaaagaagct
      421 ttaaaagaag gagaccatta tccctcctcc accatcggat tatccaacgt caatgaacga
      481 ctgcggatgg tttacggccg caattacgga ttgagcattg aaagcgaggc gaaggccgga
      541 accacggtgg aactggtcat accggccctc aaaaaggagg atttgcggga atatgtacaa
      601 ggtgttcttg gtggatgatg aaccctttat cttaaaaggg ttatatacca ttatcgattg
      661 gaacgaatac gggcttgaga tcgccggaca ggccacaaac ggccttgaag cgctggaaca
      721 gttggaaaaa aaaccggcgg atatcgtgat cgccgatatc accatgccca aaatggacgg
      781 cctcgaactg atccggcgtt taaaaagaat taacccaact accaaattca tcatcttgag
      841 cgggtataac gagtttgatt ttgtgaagac aggaatcgaa ctggggatcg aaaactacct
      901 cttaaaaccg gttaacgtcc aggaacttgg ggccacgctc accagcacgg ttcaaaagct
      961 tgaagattca gccggcaaaa tgccgttgac aaaaaaggat ttcaatattt tgcgggacaa
     1021 tatcctttac cgttgggtca cccataacat cgatccgctg gaattaaagg aacgctcccc
     1081 ttttctccaa atcggcctgg aatattcgga ttatacggtg gcgattatca acgtcatccg
     1141 ggaggagacg gaagacgatg catcgtcccg cttcaattcg gaagaaaaac tcgggcaaac
     1201 ctataaccgt tgcctggcaa tgatcgacgg gaccgttgcc ggagtctgct tttgcgatcc
     1261 gcagggtgag agcatcttca tttttgggga gagtgcaggc ctactaaatc aatcaaacat
     1321 cgatgtaaag ctggcccaac tgcaggaaga cctgaaacag acgctgaagg ttgatacctt
     1381 tattactatc ggggattccc aaaaaagctt ttataacgtt taccaaagtt atgaacacgc
     1441 caaaaagctc caagaatacc tgctggtcta ttcaagcggc aaaacactga gatttgacga
     1501 ccttaaaact cttccggcgg atggtgacgc ttcgcccgcc atcgattacg agactttttc
     1561 aaagctactg ctgtcaaaga gtcagggtga agtcttcgct ttcatcgatc aagtctttga
     1621 aacgctactg aaacgcgagg gtatccaacc gtcaaccatt caaaattgcg cggtggaaat
     1681 cgtactccat atctatgaaa cactgagcaa tttggagatc ggttgtaatc cggcaaccaa
     1741 tgattaccgg gagtttttct ttttcatctt tcaatttcgc accctggggc agttaaaaaa
     1801 ctacctaaaa aatatcgccg caagcgccat caatcgtttc agctccgaag atcaaaaaaa
     1861 gagcccggtc attaagcaag tactgaacta tatcaacctt cattatcaag atgaattatc
     1921 cctaaaaatg ctgggcagta tgtttaatgt caattccacc tatctgggcc agctttttca
     1981 gaaggagacc ggctatctct tctccgatta cgtaaaccgg ttcaagatcg aaaaagcccg
     2041 tcaaatgttg ttaaatacca atttacgggc gattgagatc tccaaaaaat cagggttctc
     2101 cgatcccaat tatttcttca aacaatttaa aaaatatgtc ggtgtttctc ccacggagtt
     2161 gaggaacttt aaaaaatcgg aatagatggt tagggcttga ccttcccttc ccgccaatgc
     2221 ttgcggcaaa gggagatata actttcattg ccgccgatct gaacctgttc tccttcgtaa
     2281 accgccttgc cgttcagtag gcgggtattc atgatcgctt tctttccgca ccagcagatg
     2341 gtcttcaact cctcaatgct gtcggccagg gcgagcagtg cctcgctccc gggaaacagg
     2401 ttgccccgaa agtcggaacg cagcccgtag gcgatcaccg gaattcccat atcgtctacg
     2461 atccggcaa
//
LOCUS       DASVMB010000266         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_695971,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000266 DASVMB010000000
VERSION     DASVMB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695971"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF531_07750"
     CDS             complement(<1..428)
                     /locus_tag="VF531_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7713904.1"
                     /translation="MEYRYRTADALGRIETGTAQAESKEQLIQKLRAEGRYLLEIREQ
                     TIPEVKFRFHRFRIQDRLSFTQQLAGLLNAGITLERALAILNELQFGQELDNVLRQLR
                     RSLEAGLSFTAALEKFPEYFPPLFINMVRAGEAGGILPGV"
     gene            complement(444..2129)
                     /gene="gspE"
                     /locus_tag="VF531_07755"
     CDS             complement(444..2129)
                     /gene="gspE"
                     /locus_tag="VF531_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX7713905.1"
                     /translation="MERLGQILMEQGWITPEILERGLKIRSEQDLPLGIILVNMGALS
                     GERLAQGLGIQLQLPYAQPLTASIDPKILERLSSRQAQRFQVIPVGIDGERLVLATAD
                     PLDLEHLDELREMLNCPFKLAVASREQIQGALERYYSGTLESVSAILEDLNEAELSEY
                     RSHAGSEVSAIAPETMANEAPIIRLVNTIIASAVKSGASDIHLEPFEDEVKVRYRIDG
                     VLQESSSPPKNLYPAVLSRIKLMAGMDITERRLPQDGRIRIKLEDRELDLRVAVAPTL
                     HGEGAVLRILNRETIMLSLDQLGLSHQNLRLLERLIARPHGMILVTGPTGSGKTTTLY
                     AALQKLNDPSRKIITIEDPVEYQLKGINQMQVNPKLNFSFAQGLRTIVRHDPDIILVG
                     EIRDRETAEVAIQSALTGHLVFATLHTNDAATAFTRLIDMGVEEFLVASTVCGVLAQR
                     LVRKVCPHCRAPYQASAEERELLGATGEEIGLFHGRGCDECNHIGYRGQIGIFEMLVT
                     DGEIERLVMARNSSGKLRDAAINSGMVTLHDDGLQKVRQGITTLAEVLRVTRD"
     gene            2390..>3167
                     /locus_tag="VF531_07760"
     CDS             2390..>3167
                     /locus_tag="VF531_07760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7713906.1"
                     /translation="MSEFLISIIIPVFNEAEMIPGSLRTIDSVLNRADISHEFIVIDD
                     GSSDASWKAIKGCAYQYRLRAIRLSRNFGKEAALCAGLETASGNATVIMDADLQHPPE
                     LLPEMVRLWRTEGYEIVEAVKISRGKENPVHHFGALIFYKLLYRLSGIDLNQASDFKL
                     LDSRVVTSWRRLGECQTFFRGMAAWMGYRRIGIPYSVPARERGDSKWTALKLFNLALT
                     AITSFSALPLQIITVLGILFLIGSVPLSFQTLQLWFRGLTV"
BASE COUNT          735 a          880 c          761 g          791 t
ORIGIN      
        1 aaaactccgg gcaggatccc tcccgcttct ccggcccgaa ccatgttgat aaagagcggc
       61 gggaaatatt ccgggaattt ttccaaagcc gcggtgaatg aaagcccggc ctccaaggag
      121 cggcgaagtt ggcggagaac attatccaat tcctggccga actgaagctc attcaagatg
      181 gctagcgccc tttccagggt aatgcccgcg ttcagtagtc ccgccaattg ttgggtgaaa
      241 ctcagccgat cctgtattcg gaaacgatgg aaccggaatt tgacttcggg tatggtttgc
      301 tcccggatct ccaggagata tcgtccctcg gcccgcaact tctggatcaa ttgttctttt
      361 gattccgcct gtgctgttcc ggtctcaatt cgccccaggg catcggcggt ccggtaccgg
      421 tattccactc cgacccctcc actttaatcc cgcgtgaccc ttaatacctc agccaatgtc
      481 gtaatgcctt gcctcacctt ttgcagccca tcatcgtgta aagtaaccat cccggaattg
      541 atcgccgcat cccggagttt tcctgaactg ttcctggcca tgaccaatcg ctcaatctct
      601 ccatcggtca ccagcatctc gaatattccg atttggccgc ggtaacctat gtgattgcat
      661 tcatcacagc cacgaccatg aaataaaccg atttcttcac cggtagcgcc caagagttca
      721 cgttcttcgg cgctcgcttg atatggagcc cggcaatggg ggcatacctt tcttaccaaa
      781 cgctgggcca gcactccaca aacggtggat gccaccaaaa actcctccac ccccatatcg
      841 atcaaccggg tgaaggcggt tgcagcatcg tttgtatgta aagtggcgaa taccagatgc
      901 ccggtgaggg cggattgaat ggccacctcg gcagtttccc gatcgcgtat ctctccaact
      961 agaataatat cgggatcatg tcgcacaata gttcgtaacc cttgggcaaa cgaaaaattc
     1021 agcttcggat tgacctgcat ctggttaatc cctttaagct gatattcgac aggatcttcg
     1081 atggtgatga tcttgcggga tgggtcgttc aatttttgta acgccgcgta aagggtggtg
     1141 gttttgccgc ttccggtggg accggtaact aaaatcatcc catggggcct agcgatgagc
     1201 cgttccaaaa gccgcaaatt ttgatgcgac aggcctaatt gatccaggct aagcatgatg
     1261 gtctcgcggt tcaagattcg caacaccgca ccttcaccat gcaacgtggg cgccaccgca
     1321 acacggagat ctaactcccg gtcttccaac ttgattcgaa tccggccgtc ttgcggcaac
     1381 cgccgttcgg taatgtccat cccggccatc agtttgatcc gtgaaagtac cgcagggtaa
     1441 aggttcttcg gcggcgatga gctctcttgg agcacgccgt cgatgcggta acggacctta
     1501 acctcatcct caaaaggctc cagatggata tcgctggccc cggattttac ggcactggca
     1561 atgatcgtgt tgaccaagcg gataatggga gcctcattgg ccatggtctc gggagcaatg
     1621 gcgcttactt cgctcccggc gtggctccgg tactcgctta gctccgcctc gttcaaatct
     1681 tccaggattg cggagaccga ctccagcgtc ccgctataat acctctccaa agccccctgg
     1741 atctgctccc ttgaagctac tgccagtttg aacgggcaat tgagcatctc ccgtaactca
     1801 tcgaggtgtt ctagatccag tggatctgct gtagccagaa ccagccgctc gccatcgatg
     1861 ccaaccggaa taacctgaaa acgttgagct tggcgggaag ataacctttc aagaattttc
     1921 ggatcaatac ttgccgtaag cggttgagca tatggcaatt gcaactgaat tcccagccct
     1981 tgggcaagcc tttcgccgga gagtgctccc atgtttacca ggattatgcc cagcggcaga
     2041 tcctgttcag acctgatttt taatccccgt tccagtatct ccggggtaat ccaaccttgt
     2101 tccatcaaaa tctggcctaa tctctccaaa cccgttctcc caaaagatat tcttgtctct
     2161 ctttaaaaaa cggttgaacc aagtacgaaa ggcaagtaca ttcgcagaca ttttcctaaa
     2221 aaagataaac catgttataa tttctacgaa agccaatgat atccttcaat tttccgataa
     2281 acgccttcca aatggtgatc gagtcttttg actatccctg aagataaatg taaaatatat
     2341 taaaaagttt ccaacttcca ccctaatttt accgaggagg aaacgggtag tgtccgagtt
     2401 cttaatctcc atcatcatcc cggtatttaa cgaggcggaa atgattcccg gaagtttacg
     2461 gaccatcgat tccgttttga acagggcgga tatctctcat gagtttatcg taatcgacga
     2521 tggctcaagc gacgcttcgt ggaaagcgat caagggatgc gcataccagt accggctccg
     2581 tgccattcgt ctcagtcgga acttcggtaa ggaggcggca ttatgcgccg ggttggaaac
     2641 agcctccggt aatgcaacgg taatcatgga tgccgatctt cagcatcctc cggaactcct
     2701 cccggagatg gtccggttgt ggaggaccga gggctacgaa atcgtggaag cggtcaaaat
     2761 ctcccgtggt aaggagaacc ctgtccacca cttcggggcc ttgatttttt acaaactcct
     2821 gtatcggtta tccggtatcg acctgaatca agcctccgat ttcaaattac tcgattccag
     2881 agtggttact tcatggcggc ggctgggaga atgccaaacc ttttttcggg gtatggccgc
     2941 ctggatgggt taccggcgga tcggcattcc atactcggta cctgcccgcg aacgggggga
     3001 ttccaaatgg accgctctga aattgttcaa cctggcgtta acggcgatca cctcattctc
     3061 cgccttaccg ctacagatca ttacggtcct ggggattctc ttcctgatcg gctctgttcc
     3121 actcagcttc caaaccctgc agctttggtt cagaggcttg acggtca
//
LOCUS       DASVMB010000267         4719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_108498,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000267 DASVMB010000000
VERSION     DASVMB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108498"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VF531_07765"
     CDS             complement(<1..1225)
                     /locus_tag="VF531_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7713907.1"
                     /translation="MNIRSKRIRWAFFVMVCFIGMGTVGFKLNLFTGNTANTSQVYAA
                     QGPAGVEGPEGNSKDTIQSGTPLIPVTGNQSAVAIVQSREAKAENIKYEEIRTMVRTA
                     VEMAGGFQGLIKNGQTVVIKPNLVTPIDYTLPGWGGRPLAPEVNGTTTDYRVTRAVVE
                     LVRRYNPDGKVYIMEGSAFPTRECMKQLNYTPKFIPGVDEFVCIEEDSGNWRDFNSSR
                     IVKVPVPDHLLAKEVYMNRRYKEADVVISVPALKNHWNAVTSGSIKNVSIGATPATIY
                     GGGDPGMLNRGGGIDHTKEKLDKWIHDWFMARPVDFTIMDGLQGIQNGPTPSFDMSRT
                     SDIRQDQMNTRLILASRDCVAMDSIESLVMEVDPKAVTYLGYLEKSGAGKSDVAAIRV
                     VGKRVDEVRKNFKGIL"
     gene            complement(1259..2161)
                     /locus_tag="VF531_07770"
     CDS             complement(1259..2161)
                     /locus_tag="VF531_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7713908.1"
                     /translation="MHSYSPIQSLLLILYFSGNALLELAPYVIGGVIIGEALKLTSWT
                     KLIYGGVSRSPLLATLFASVLGAVSPLCTYGTVPVVLQLFRAGVHIAPLITFLSVSAM
                     MNPQLFILTWGGLGPEMAIVRVLAVMGFGLFLGFSLYRLPASWVVNPAAMDEKEPPSK
                     ILNRSAHPFTVKGFSKDVLETLQFVGFYLVLGVILSATIDVIIPKQWLRGFFSGNRFI
                     SLLSSALLGVPLYACGGGVIPVVRELMANGMAKGAAMAFMLVGPATRVTPLIALATIL
                     RPRFIFIYILAVITFAVLFGFVYQ"
     gene            2359..3018
                     /gene="lepB"
                     /locus_tag="VF531_07775"
     CDS             2359..3018
                     /gene="lepB"
                     /locus_tag="VF531_07775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7713909.1"
                     /translation="MLIRLWRFLIGKPEKPLSESPWFVRCLWELRSLVLLFGLTFFII
                     QPFVIAGYDTPSGSMEPTIMTNTRYIALPSIYGGFFPFSKIKLPGFKKIQRGDIVIFK
                     YPVDERQNFVKRVIGLPGEEVQVKGESVSINGQVLNEPYAHFDDSVGENPVSRNFGPV
                     TVPPNHLFVMGDNRDISYDSRYWGFVPVKNVFGTPLFTYWSFDNERHQVRLQEIFKGI
                     R"
     gene            complement(3196..4056)
                     /locus_tag="VF531_07780"
     CDS             complement(3196..4056)
                     /locus_tag="VF531_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3919 family protein"
                     /protein_id="HEX7713910.1"
                     /translation="MRNHLFIIGLVFCCIVFLLYGLITYWDFVIADFGIAVDKAVVIP
                     DNHKVDELIAKNTPVRVEIVDERWGKTVIEDPVILFNIWNLLREPKTVPSKYLVNRNR
                     INGYIYFYDGSKQFFSISDQFQLGDSVIENSETEELDVNKLYRILQYNLASQQNLIKM
                     VETADAVYLYKANDFFDPESDRMLKLTGKIKQQFLPYIAKSHKVVDSNELNHFLLDGD
                     SSPLFHIALSFKEGDSVRLVIISVLSDKYFNVMDMSYIDRNIIYFEGPLGDFCKEIFD
                     TPFAMMEIGP"
     gene            complement(4056..>4719)
                     /locus_tag="VF531_07785"
     CDS             complement(4056..>4719)
                     /locus_tag="VF531_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7713911.1"
                     /translation="FNNMTHEIRTPLTTIIGYADIIEKLDTKEERSDCLQYIQTEGKR
                     LLRLINDLFRLSKLNTYTLTLSQVQCSLERILQETAEIVRYKANKYGIVINTSIKTNV
                     KLKADKDKLKEVLLNLVDNAILHSKSPQIDIMLSIREKEALIEVKDYGQGIGAEQMEK
                     IRQKFSGCPINSIYDQAGHGYGLLVSKSIIEAHGGSLEIDSTEGTGTTVRVRLPIMKG
                     EA"
BASE COUNT         1295 a         1146 c          993 g         1285 t
ORIGIN      
        1 ttaagatacc cttgaaattt ttcctcactt catccacacg tttccccaca acacggatgg
       61 ctgcgacatc gcttttaccg gcgccgcttt tttccagata acccaggtag gtaacggcct
      121 tcggatccac ttccatgaca agggactcga tgctatccat cgcgacacag tcgcggctcg
      181 ccagaatcaa tctggtattc atttgatcct gtcggatatc cgaagttctg gacatgtcga
      241 aactgggggt cggcccgttt tggatgcctt gcaggccatc cataatcgtg aaatccaccg
      301 gccttgccat aaaccaatca tgaatccatt tgtccaattt ctctttagta tggtcgatac
      361 caccgccgcg attgagcatt ccgggatctc ccccgccgta gattgtggcg ggagttgcac
      421 cgatactgac atttttgatt gaacccgagg tgactgcgtt ccaatgattt ttcaatgccg
      481 gaacgctgat tacgacatcc gcttccttat agcgcctatt catgtatact tctttggcga
      541 gcaaatgatc gggcaccggt actttaacaa tccgggatga attaaaatcc cgccaattac
      601 cgctgtcctc ttcgatacaa acgaactcat cgactccggg aataaacttc ggggtataat
      661 tcaactgttt catgcactcc cgggtcggaa aggccgaacc ctccatgata taaacctttc
      721 catccgggtt ataacgacgg accagttcca caacggccct ggtgacacgg taatccgtgg
      781 tagtgccatt cacttcgggg gctagaggcc ggcctcccca tccgggaagc gtatagtcga
      841 tgggtgtaac cagattgggt ttaatcacaa cggtctgacc atttttaata agaccctgaa
      901 acccgccggc catttcgaca gcagtacgga ccattgtccg gatctcttca tatttgatgt
      961 tctcggcttt ggcttctctt gactggacga tggccactgc cgactggtta ccggtaaccg
     1021 gaatcaaagg agtacccgat tggatcgtat ccttgctgtt tccctccggt ccctctaccc
     1081 ctgccggacc ttgggctgca tatacttgag aggtgtttgc agtattccct gtaaataagt
     1141 tgagtttgaa tccaacagtt cccatcccaa tgaaacatac catgacaaaa aaagcccatc
     1201 ttattcgttt cgatcttatg ttcattcgtt tcgcctccta atcagtcatt taagagatct
     1261 attgatatac aaacccgaaa agaacagcaa aggtgataac agccaaaatg tatatgaaga
     1321 tgaatctcgg ccgcagaatc gtcgccaaag caatcaaggg cgtaacccgg gtggctggcc
     1381 caaccagcat gaatgccatg gcggctcctt ttgccatacc gttcgccatc aactcccgga
     1441 ccaccggaat gacccctccg ccgcaagcat agaggggaac accaagcagt gccgaggata
     1501 acagcgagat aaaccggttg ccgctaaaaa aacctcgcag ccattgtttg gggataatga
     1561 cgtcaatggt tgcgctcaaa atcactccca agaccagata aaacccgaca aactgcagcg
     1621 tttccagcac atctttggaa aaacccttca ctgtaaaggg atgggccgaa cggttcagta
     1681 tttttgacgg tggttctttt tcatccattg ccgcagggtt gacgacccat gatgccggta
     1741 gccggtagag actgaacccc agaaataacc cgaatcccat aacagccaaa acccttacga
     1801 ttgccatctc gggtccgagt ccaccccagg tgaggataaa tagttgggga ttcatcatgg
     1861 cggataccga aagaaacgta atcagcggag cgatatgtac tccggcccga aagagctgca
     1921 ggacaaccgg aacggtgcca taggtacaca agggagaaac tgcgcccagc accgaggcga
     1981 atagcgttgc aagtaatggt gagcgggaga ctccaccata tatcagtttt gtccaggagg
     2041 tgagcttgag cgcttcaccg ataattactc ccccaataac atagggagcc aattcaagca
     2101 aagcattgcc cgaaaaatac agaatcaaaa gtaaagactg gatgggggaa taagaatgca
     2161 tcaaaacctc atttgtttta gagtgggtta cggataaatc atactgtacc gttccataat
     2221 attgatacaa ttctattata tctcaattat ttcaagcctg atagccaaat aataagaaga
     2281 atggataacc ataaaagaaa cttatggtaa gtttttccgt ttaaatggct atatgtacca
     2341 tttataaggg gtgatcctat gcttattcga ctatggcgat tcctcatcgg taaaccggaa
     2401 aagccgctct ccgaaagtcc gtggttcgtt cgctgccttt gggaactccg ttcactggta
     2461 ttgcttttcg gcctaacttt tttcatcatt caacctttcg tgatagccgg ttatgataca
     2521 ccatccgggt cgatggagcc gaccatcatg accaataccc gttacattgc tttaccatcc
     2581 atttacggcg gattctttcc attctcgaaa ataaaacttc cgggatttaa aaagatacag
     2641 cgcggggata tcgttatctt taaatatccg gttgatgagc ggcagaattt cgtgaaacgg
     2701 gtcattggct tgcccggaga agaagtccag gtgaaaggag aatcagtatc cattaatggc
     2761 caggttctga atgaacccta tgcccatttt gatgattccg ttggcgagaa tccggtaagc
     2821 cggaatttcg gtcccgtaac ggttcctcca aaccatctgt ttgtaatggg tgataaccgc
     2881 gatatctcct acgattcgcg ctattgggga tttgtgccgg taaaaaatgt tttcggcacg
     2941 ccgctattta cctattggag ttttgataat gaacggcatc aagtgagact ccaagagatc
     3001 tttaagggaa ttcgctgacc ggagccatcc aaaacccgtt tgaattaaga tgaaatgatc
     3061 tgtgctttgc aataaaaaga aacgaacaat accggtttta accggtaaga attattacag
     3121 ccactggttt taaccagtgg cttgagatgt caaggtttat cctgagctaa atggatagtc
     3181 attttaaatg ataaactaag gcccgatttc catcatcgcg aatggagtgt caaatatctc
     3241 cttgcagaaa tcacccaaag gcccttcaaa ataaataata ttcctgtcaa tataactcat
     3301 atccatcaca ttaaaatact tatccgacaa tacggagatg ataaccaatc ttacagagtc
     3361 tccttctttg aaagataggg caatatggaa aagcggagat gagtcgccat ccagtaaaaa
     3421 atgattgagc tcattactgt caacaacttt atgggatttt gcaatgtacg gcagaaattg
     3481 ctgcttgatt ttcccggtaa gcttcaacat cctatccgat tccggatcga aaaaatcgtt
     3541 ggctttataa agataaaccg catcggccgt ctccaccatc tttatcaggt tctgttgtga
     3601 cgccaaatta tactggagga tgcggtataa tttatttaca tccagctctt ccgtttcact
     3661 attttcgatg actgaatcac ccaattgaaa ctgatcggat atactaaaaa attgtttact
     3721 gccgtcataa aaatagatat acccattgat ccggttccgg ttgaccaggt atttactggg
     3781 aacggtttta ggctcgcgca gtaaattcca gatgttgaag aggattacag gatcttcgat
     3841 gactgtcttt ccccatcgtt catcaacaat ttctacccta accggtgtat tcttggcaat
     3901 caattcatcc accttatgat tgtccggaat gactaccgcc ttatccacgg caataccaaa
     3961 gtcagcgatg acgaaatccc aatacgtaat taacccgtaa agcaaaaaaa cgatacaaca
     4021 aaagaccaag ccgataatga acagatgatt acgcattaag cttcaccttt cattattggt
     4081 aatcttactc tgaccgttgt ccccgttcct tccgtactgt ctatctccag actgccgcca
     4141 tgcgcctcaa tgatactctt tgaaacaagc aagccgtaac cgtggcccgc ttggtcatat
     4201 atcgaattaa tcgggcaacc ggaaaacttt tggcgaatct tctccatttg ctcggccccg
     4261 ataccctgcc cgtaatcctt aacttcaatc aaggcttctt tttccctgat ggatagcatg
     4321 atatctattt gcggggattt gctatgtaaa atcgcattgt caaccaagtt tagcaggact
     4381 tctttaagtt tatccttatc cgccttcaat ttaacatttg tcttgattga agtattgatc
     4441 acaattccat acttatttgc cttgtacctg actatttccg ctgtttcctg caagatcctc
     4501 tccaaactgc attgaacctg gctcaaggtt aaagtatatg tgttcaattt ggataggcga
     4561 aacaggtcgt taatcaagcg cagcagtcgc tttccctcag tttgaatata ttggagacaa
     4621 tcgcttcgtt cttctttcgt gtccagtttt tcgatgatgt ccgcataacc aatgattgtc
     4681 gtaagcggcg ttctgatttc gtgggtcata ttgttaaag
//
LOCUS       DASVMB010000268         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_474838,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000268 DASVMB010000000
VERSION     DASVMB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474838"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF531_07790"
     CDS             complement(<1..518)
                     /locus_tag="VF531_07790"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713912.1"
                     /translation="MKFRTRLFLFYIGAVGCIAAALAVYSISFEERQMYQSLHEQLVV
                     DARLITGSFADPLILRDSLRTNRIVADIARKTKLRITVIAADGRVLGDSSQDYTRMPN
                     HRNRPEVRTAFLGGEGFITRYSQTLKETLIYSALPVQLENGTIIGVVRVAKSLSQVKR
                     QLLRIRALIAGG"
     gene            complement(527..1219)
                     /locus_tag="VF531_07795"
     CDS             complement(527..1219)
                     /locus_tag="VF531_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7713913.1"
                     /translation="MAKRILIIDDEPMIVESVSYNLKQQGYEVIASGDGETGLKLAQS
                     ETVDLILLDLMLPGIDGLEICRILRQHSEVPVIMLTAKEAEIDRVLGLELGADDYITK
                     PFSMRELLARVKSVLKRTAASGGNYTGQSQTINSGELMIDPLGHEVTLGGRTVNLTSK
                     EFELLRILATHPGQVLTREQLLNLVWGSDFFGDDRTVDVHIRWLREKIETDPGDPTYI
                     LTVRGAGYKFRK"
     gene            complement(1289..1945)
                     /gene="phoU"
                     /locus_tag="VF531_07800"
     CDS             complement(1289..1945)
                     /gene="phoU"
                     /locus_tag="VF531_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7713914.1"
                     /translation="MTREGLDRSLRDLQQGLLRMGSMVEEAIHLAVESLAKQDLQLAQ
                     RIIDGDDAIDTLSLAIEEDCIRLIALQQPIAKDLRVITTVLKTVTDLERIADHATNIA
                     EITQRIGTAELIKPLIDIPEMARLVEAMVHDSLKAFVDRDVDLAKATCLKDEEIDRIF
                     EGLLNELTDFAMAGDDPKRVAQAMSLVFAARYLERVGDHATNIGERVIYLVTGRRERY
                     "
     gene            complement(2018..2773)
                     /gene="pstB"
                     /locus_tag="VF531_07805"
     CDS             complement(2018..2773)
                     /gene="pstB"
                     /locus_tag="VF531_07805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7713915.1"
                     /translation="MDIKIKAEDFSLYYGDFQALQAINMPIFSRTVTALIGPSGCGKT
                     TFLRSLNRMNDLIDNVRVEGRVYLDDKEIYEPKQDVVTLRKRVGMVFQRPNPFPMSIY
                     DNITYGPRIHGIKDKTLLNEIVERSLQGAALWNEVKDRLHSPALRLSGGQQQRLCIAR
                     LLAVEPEVLLMDEPASALDPISTAKIEDLITELKQNYSIVIVTHNMQQAARVSEYLGF
                     FLLGRLVEFGPTETVMTNPAEKQTEDYLTGRFG"
     gene            complement(2944..3762)
                     /gene="pstA"
                     /locus_tag="VF531_07810"
     CDS             complement(2944..3762)
                     /gene="pstA"
                     /locus_tag="VF531_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7713916.1"
                     /translation="MQRAAFILLWCTAVVTIGCLVLIVVFILRKGFPSINWEFLTAFP
                     KNMGREGGIFPTIVASLYLTIVSLIIAAPIGVGAAIYLTEYTREGWLTRVIRFATETL
                     AGVPSIIFGLFGFAFLVTFMHLGFSILSGAITLALMLLPTIIRTSEEAIKTVPASYRE
                     GSLALGATQWTTIAKVVIPAALPGIVTGLVLSVGRAIGETAAVWLTVGGALRLPFSVM
                     DSARPMTLHLYTLAAEGLSLPRAYATASVLIITIWIINSVADILMYRVMSRLRG"
     gene            complement(3780..>4270)
                     /locus_tag="VF531_07815"
     CDS             complement(3780..>4270)
                     /locus_tag="VF531_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7713917.1"
                     /translation="VILVPAVRGIFGGVGLSLLTGGIILAIMILPTIISISEDSLRAV
                     PKDYKEASLALGATHWQTIYRVLVPAARSGITAGVILGMGRAIGETMAVIMITGNTPL
                     IPKSILEPGATLTGTIGMEWAYASGEHAQALFTVGIFLFILIIIINSTALWFSQRKVN
                     RS"
BASE COUNT         1065 a         1202 c         1024 g          979 t
ORIGIN      
        1 ataccgccgg ctatcaaagc ccggatccgg agtagctgcc gtttgacctg gctcaggctt
       61 tttgcgaccc gaaccacccc gatgattgta ccattctcca gttgtaccgg cagagctgaa
      121 tagatcaacg tctccttcaa cgtctggctg tagcgggtaa taaaaccctc accccccaga
      181 aaggcggtcc gcacttcagg acggttacgg tggttcggca tccgggtgta gtcttgggaa
      241 gaatccccca acacccggcc gtccgctgcg ataaccgtaa tccgcagttt ggttttacgg
      301 gcgatatcag ccactatccg gttagtccga agactgtccc ggagtatcaa agggtcggca
      361 aaactcccgg tgattagccg ggcgtcgacg accaactgct cgtgcagact ttgatacatc
      421 tgtcgttcct cgaagctgat ggaatacacc gcaagcgccg cggcgatgca gcccacagcg
      481 ccgatataaa aaagaaagag ccgggtcctg aatttcatgc tggcctctat ttcctaaatt
      541 tatagcccgc tccccggacc gtaagaatat aggtgggatc tccgggatcc gtctcgatct
      601 tctcccggag ccagcggatg tgaacgtcta ccgtccggtc gtccccaaaa aaatcacttc
      661 cccagaccaa attcaataat tgttccctgg ttaatacctg cccgggatgg gtagccagga
      721 tccggagcaa ttcaaactcc ttactcgtca aattaactgt ccggccgccc aaagttactt
      781 catggccaag cggatcgatc attagctccc ccgaattaat cgtttgagac tggccggtat
      841 agtttcctcc cgaggctgcg gtccgtttca ggaccgattt gacccgcgcc agcaattccc
      901 gcatactaaa gggtttcgta atataatcgt cggccccgag ttccaggccg agaacccggt
      961 caatctccgc ttccttggca gtcagcatga tgactggcac ctcggaatgt tgccggagta
     1021 tccggcagat ttcaaggccg tcaatgcctg gaagcatcaa atcaagcaag atcagatcta
     1081 cggtttccga ttgagcgagt ttcaggccgg tttcaccgtc accagacgcg atgacttcgt
     1141 aaccttgctg tttcagattg taactgaccg actccacgat catcggttca tcatcaataa
     1201 tcagaatccg tttcgccatc cctgttcctc catgggatta atcacgcgcg tgggcgcgtt
     1261 cgcaacgcga acgagagaaa cccgacaatc agtaacgctc tcttctccct gtaaccagat
     1321 agatcacacg ttcgccgatg ttggtggcat gatcgccgac tcgttccaga taccgagcgg
     1381 caaatactag actcatcgcc tgggctaccc gcttcggatc atccccggcc atggcgaaat
     1441 cggtcaactc attcaggaga ccctcaaaga tcctatcaat ctcctcatcc ttgagacagg
     1501 ttgccttggc cagatcaacg tctcgatcca cgaaggcttt gaggctgtca tgcaccatcg
     1561 cttcgaccaa acgggccatt tccgggatat cgatcaacgg cttgattaat tccgccgtac
     1621 caatcctttg ggttatctcg gcgatattgg tggcatggtc ggcgattcgt tccagatcag
     1681 tcactgtctt taaaaccgtg gtgataaccc tcaaatcctt ggcaatgggt tgctgtaaag
     1741 cgatcaaccg gatgcaatcc tcttcaatgg cgagcgaaag cgtgtcgatg gcatcgtcgc
     1801 cgtcaataat tctctgtgcc agttgcagat cctgcttggc caaggattca accgccagat
     1861 gtatggcttc ttcaaccata cttcccatac gtaacaaccc ctgctgtaaa tcacggagcg
     1921 atctgtccaa gccttccctt gtcattccga tcaccaccta ttcgttattc taattatatc
     1981 caagatccag tccctattcc agtaatatcc ctcatcctta accgaaacgg ccggtcaaat
     2041 aatcctcagt ttgcttctcc gcgggattgg tcatcaccgt ctcggtgggg ccgaactcta
     2101 ccaaccggcc caggagaaag aaacccagat attccgagac ccgcgccgcc tgttgcatat
     2161 tgtgggtaac aatcacaata gaataattct gtttcaattc ggtgatcaaa tcttcaatct
     2221 tggcggtgga aatcggatcc aaagctgatg ccggttcatc catcagcaat acctccggtt
     2281 ccactgccag taacctggca atacacaatc tttgttgttg gccgccggaa agccggagtg
     2341 ccggcgaatg gagccgatct tttacttcat tccaaagtgc cgctccctgc aggctccgtt
     2401 cgacgatctc attcagtaag gtcttgtcct tgataccatg aattcgtggc ccataagtga
     2461 tattgtcgta gattgacatc ggaaaaggat tgggccgttg gaaaaccatg cccacccttt
     2521 tacgcagggt aaccacatct tgtttgggtt cgtatatctc cttgtcatcc aggtaaaccc
     2581 gcccctccac cctcacatta tcaattaaat cattcatccg gttcagtgaa cggagaaagg
     2641 tggttttgcc gcaacctgat ggcccgatta acgcggtgac cgttcgggaa aaaattggca
     2701 tattgatagc ctgtaatgcc tgaaaatcac cgtagtatag actaaagtct tccgccttga
     2761 ttttgatatc cattggtaag cctccttaga agtaggcaat agggatcgcg cccaatgggc
     2821 gcggggcaac aggcaacagg ggtaaaccac tgcctttaac ttttgatttt atccataaca
     2881 atcgatcgag gacaaaagat tacctaagta ctgttgccta ttgccagttg cctgttccct
     2941 agttcatccc ctgagccgcg acatgacccg gtacattaaa atatcggcga cggaattaat
     3001 gatccagatg gtgatgatta agacgctggc tgtagcgtaa gcccggggta atgacaaacc
     3061 ctccgcagcc aaggtgtaga gatgtaacgt catcggccgg gccgaatcca tgactgagaa
     3121 cggtaaacgc aaggcgccgc cgacagtcaa ccagactgct gccgtttcgc cgattgcccg
     3181 gccaacactt aaaaccagcc cggttacaat acccggcagg gccgcaggta tgactacctt
     3241 ggcaatggtg gtccattgag ttgctcccag ggccagactg ccctcacgat atgaggcggg
     3301 taccgtttta atagcctcct ccgaggtgcg gatgatggtc ggcagtaaca tgagagccag
     3361 ggtgatcgca ccggatagaa ttgaaaatcc caaatgcatg aaggtgacca aaaaagcgaa
     3421 accgaacaga ccgaagatga tcgaagggac tcccgccaaa gtctcggtgg cgaaacggat
     3481 cacccgggtc aaccagccct cccgggtata ctcggtcaga tagatcgccg cgccgactcc
     3541 tatcggggcc gcgataatta gagacacaat ggtcagatat aatgacgcga caatcgtggg
     3601 aaaaatcccg ccctcccttc ccatgttctt gggaaaagcg gtaaggaact cccaattgat
     3661 ggaaggaaac cccttcctta atataaaaac cacaatcagt accaaacagc ctatggtgac
     3721 taccgccgtg caccataaaa ggatgaaggc agcccgttgc atgtttgcag aagatgattt
     3781 caagatcggt tcaccttcct ttgagaaaac cacaacgcag tagagttgat gatgatgatc
     3841 aagataaaca gaaagattcc cactgtaaaa agggcctgag catgctcgcc cgaggcgtac
     3901 gcccattcca tgccgatggt cccggttaaa gtagcgccgg gttccaaaat ggatttgggt
     3961 ataagcgggg tattgccggt gatcatgatc accgccatgg tttccccgat tgcccggccc
     4021 attcccagga tgacccctgc ggtaattccc gaccgggccg ccggcaccaa aactctatag
     4081 attgtctgcc aatgcgtcgc ccccaaagcc agggaagcct ccttgtaatc cttgggcact
     4141 gcccggaggg aatcctcgga gatgctaatg atggtcggga gaatcatgat cgccaaaatg
     4201 atgccgccgg tcaacaggct taatcccacc ccgccgaaga taccgcgaac cgcgggaacc
     4261 aatatgacca
//
LOCUS       DASVMB010000269         5648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_399931,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000269 DASVMB010000000
VERSION     DASVMB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399931"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VF531_07820"
     CDS             <1..682
                     /locus_tag="VF531_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7713918.1"
                     /translation="LFLDNEAQEYQSFIDVGANGIFYQGSFKGEPLPDNGSNKDFYTS
                     NEAVQIALTIQNQRTQLYVNKILAMNTTGFNPVMPTKLRIGFLSDASLRVKDFTVATG
                     IPDIGGELLKQGKYISHGIRFDPGMAQIKEESYSVLKQIAGVLQDNPDVKLMIVGHTD
                     NTGTVENNQKLSLTRADNVKEYLISRFKVPADRLQTSGKGDSEPITDNKTADGKALNR
                     RVEFIRVQ"
     gene            767..1981
                     /locus_tag="VF531_07825"
     CDS             767..1981
                     /locus_tag="VF531_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7713919.1"
                     /translation="MRKWGVMLSVITLLFGVTMGVSIAANQELDQQLILFAQGDFETV
                     KQLVAQGADVNARDQNGVTPLMSAASGGQTEIASFLISKGADVNSKNKSGFTPLICAA
                     MSGNQQVVQLLIDKGADVNAKDKEGYTAAYYASMYQYNDLAEMLMATGKSSSGSKGTP
                     AQTQKELDEAVLFAMANKDYNRVMNLIERGASVKVRYPDNEDSLLHAAVDQGSSTMVE
                     YLLGKGLDVNTRQKDGETPLYYALVHKQVALVNLLLNKGAMVDIKNEVDRSPLYFFVT
                     NCHENFSTEDRQIAELLIKRGADVNQKNCFDNSMAGNQTALHSAALSGNLGFVEFLLE
                     KGASINVRDNAGATPLHYGVSSGNPEVVALLIAKGADPNIRDGAGMTPLRWAEEKNDS
                     VMMAMLKKAGAK"
     gene            complement(2019..2666)
                     /locus_tag="VF531_07830"
     CDS             complement(2019..2666)
                     /locus_tag="VF531_07830"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX7713920.1"
                     /translation="MAFRYIALKTDMIHSRALHERDKIQELFFAAADRINTDFGSCFE
                     SGFIITHGDEAQALIRREAAASVYPIIESLVEMMKPVELRFGLGWGTLSTKMQKMAIG
                     MDGEAWQNAKEAIDIARKSRRWVCFRGFSFEMQKGLESLANLLLFIHGHWTPEQLQAI
                     GPIRTGQTQATIAKTLHVTEAAISKRLSAAGWREYQDGREALQELMTKVVLTLES"
     gene            complement(2672..3544)
                     /locus_tag="VF531_07835"
     CDS             complement(2672..3544)
                     /locus_tag="VF531_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713921.1"
                     /translation="MDLVANGWFFVTVVLGAEYGMKEFFELLPASLLKKKRIYWLYRL
                     LHWALLTCLLGVFFTSPYPWFIWPLTSLGASLLGSVELVGQLLEQRLRKVIQANSYFS
                     QFLLMLIEGWVAFQTAMMIRCRTGKLLTSFIDSFQVHFGTEHPFTGGEMLAVCAVVIL
                     LAYPVNYVIRWLVSKSKDNTLPELIICYQGPDRSVQEPVTVSEVNPADTSPSPSIETI
                     RAGRVIGILERWLIMVLMLSGQYAGIGFVLTAKSIARFKNFDQEQFAEYYLMGTLYSA
                     LFAIGLSLILINLG"
     gene            4083..4661
                     /locus_tag="VF531_07840"
     CDS             4083..4661
                     /locus_tag="VF531_07840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7713922.1"
                     /translation="MNQPSSSPTRCPWCLGHPLLIEYHDKEWGTPVHDERQHFEFLTL
                     EAMQAGLSWFIVLQKREIFREALEDFDYHKIAGYGEKDMERIMGFPGIIRNRRKIEAA
                     IHNARMFLAIRKEFGSFDNYIWGFVNFKPVLHSFKTQSEVPVTTELSDRISQDLKKRG
                     FKFLGSVTIYAHLEAIGVVNDHLVSCFRYGGK"
     gene            complement(4802..>5648)
                     /locus_tag="VF531_07845"
     CDS             complement(4802..>5648)
                     /locus_tag="VF531_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7713923.1"
                     /translation="SAADLSPAQKNGLEIHFVSLSFTLDGKTYHCGVDIQSDEFYRLL
                     ESSKSFPSTSQPSAGEFAGLYRRLAETDAEILSFHISSGLSGTINAARIGAAAVPEAD
                     VTLVDTKTLSGAQGWQVEAAAKAVKAGWSKERILTMVKEISDATETIFTLPSLKYLIH
                     GGRINHLKGLLASILNIMPIIGVEKEGGTYVQRGKVRTMAAAFSEMVELVKERHTPGS
                     LLRLQILHGNNAAGVELLKQKLERLYKCNWLPSGMIAPALGAHTGPGLVGLIFASESV
                     FPIIA"
BASE COUNT         1560 a         1407 c         1326 g         1355 t
ORIGIN      
        1 gttatttctg gataacgagg cacaagagta tcaatcattt attgacgttg gggcaaatgg
       61 tatcttctat caagggagtt ttaaggggga accgctaccg gacaacgggt cgaataaaga
      121 tttttatacg agtaacgaag cggtacaaat cgccttaacc attcaaaacc aaaggactca
      181 gctatatgtg aataaaatcc tggccatgaa caccaccggt ttcaatccgg tgatgccgac
      241 gaagctacgg atcggatttc taagcgacgc ttccctgaga gtgaaagatt ttacggtagc
      301 tacagggata ccggatatcg gcggtgagct cctgaaacag gggaagtaca tctcccatgg
      361 gatacgcttt gacccgggaa tggcgcaaat taaggaggaa tcctattcgg ttttaaaaca
      421 gattgccggc gtcctccagg ataatcccga tgtgaaattg atgattgtcg gccacaccga
      481 taataccgga actgtggaaa acaatcaaaa actttcgctt acccgggcgg ataacgtaaa
      541 agagtatttg atttcccgtt ttaaggtccc ggccgaccgg ttacagactt ccggaaaagg
      601 cgacagtgag cccatcaccg ataataaaac agcggacggc aaggccttaa accgccgggt
      661 cgaatttatc cgggtccaat aaaggtaccc cataaattac tctcgcttga tctttggtta
      721 tccatttgat taactgagat cctattgagc ccaaggagga atccgcatga gaaaatgggg
      781 tgtaatgtta tctgttatca ccctgctatt cggtgttacc atgggagtct caatcgccgc
      841 caaccaggaa cttgatcaac agctaatctt gttcgctcaa ggggactttg aaactgtaaa
      901 gcaattggta gcacaaggag ccgatgttaa tgccagggat caaaacggtg taacgccgtt
      961 aatgtctgct gcaagcggag ggcaaaccga gatcgcatca tttctcatct ctaaaggcgc
     1021 cgatgtcaac agcaaaaata aaagcgggtt cactcccctg atctgtgcgg cgatgagcgg
     1081 gaaccagcaa gtggtccagt tattgatcga caagggagcc gatgtcaatg ccaaggataa
     1141 agaaggatat accgccgcct attacgcctc catgtaccaa tataacgacc tcgccgagat
     1201 gttgatggct acggggaaat catcctccgg ctcaaagggg actccggctc agactcaaaa
     1261 agagctggac gaggcagtgc tttttgcgat ggcaaacaag gactataacc gggtgatgaa
     1321 ccttattgaa cggggcgcct cggtaaaagt ccgttacccc gataatgagg attcattgct
     1381 ccatgctgcg gtcgatcagg ggagttccac catggtggag taccttctcg gcaaaggttt
     1441 ggatgttaat acgcggcaaa aagatggcga aacgccactc tattatgctt tggtccataa
     1501 acaagtcgcg ctggttaatc ttcttctgaa taaaggggcc atggtcgaca tcaaaaatga
     1561 ggttgatcga tcaccattgt atttttttgt gaccaactgt catgaaaatt tctcaacgga
     1621 agaccggcag atcgcggaac ttttgatcaa acggggtgca gacgtaaacc aaaagaattg
     1681 ctttgataat tcaatggccg gtaaccaaac tgcgcttcat tccgccgcgc tttccggtaa
     1741 tctgggattt gtcgagttct tgctggaaaa aggagcatca atcaacgtcc gggataatgc
     1801 cggggcaact cccctgcatt acggggtttc cagtggcaat cccgaagttg ttgcgctatt
     1861 gattgccaag ggggccgatc cgaatattcg ggatggagcc gggatgactc cattacgctg
     1921 ggccgaggaa aaaaatgact ccgtgatgat ggcaatgctg aagaaagcgg gagcaaaata
     1981 agtttaaaac ataaactccc aaggttaacc taccgttctt aactttccag ggttaatact
     2041 actttcgtca ttaattcctg caaagcttcc cgtccatctt gatactcccg ccatcccgcg
     2101 gccgaaagcc ttttggatat cgccgcttcc gtcacatgaa gcgtcttggc gatggtagcc
     2161 tgggtttgac ctgtccggat gggccctatc gcttgcaact gttcgggagt ccagtgcccg
     2221 tggataaata ggagcaaatt ggctaaagac tccagcccct tctgcatttc gaacgaaaag
     2281 ccccggaagc aaacccagcg gcgggattta cgcgcgatat caatcgcctc cttggcgttt
     2341 tgccaagcct caccgtccat tccgatggcc attttctgca ttttggtgct taatgtcccc
     2401 catcccaagc cgaaacgcaa ctccaccggc ttcatcattt caaccaatga ctcgataatt
     2461 ggataaaccg aagccgcagc ctcccttctg ataagggctt gtgcctcatc accgtgggta
     2521 ataatgaatc ccgattcaaa gcatgaaccg aaatccgtat tgattcgatc ggccgctgca
     2581 aaaaacaatt cctggatctt atcccgttca tgtaaagccc gggaatggat catatccgtt
     2641 tttaaggcga tataccggaa ggccataacc ttcaccccaa gttgattaag atcaaactca
     2701 aaccaatcgc aaatagcgcg ctatacagcg tgcccattaa ataatactct gcaaactgct
     2761 cctgatcaaa attcttgaac cgggcgatcg atttggcggt cagtacgaat ccgatacctg
     2821 cgtattgccc actgagcatt aaaaccatga ttaaccagcg ttccaagatc ccgatgaccc
     2881 gacccgcccg gattgtctca atactcggag atggagaagt atcggccgga ttaacttcgg
     2941 atacggtaac aggctcctga actgatctat ccggaccctg atagcaaatg atcaactccg
     3001 ggagggtgtt atcttttgat ttactgacta accagcgaat cacataattc accggatagg
     3061 caagcaagat caccaccgcg catacagcca gcatttcgcc cccggtaaac ggatgttctg
     3121 ttccaaaatg tacttgaaaa ctatcgataa atgaggttaa aagttttccc gttcggcacc
     3181 gaatcatcat ggccgtctga aaggccaccc aaccttcaat gagcatgagg agaaattggg
     3241 agaaataact attggcctgg ataacctttc gtaatctttg ctccaataat tgtccaacca
     3301 attcaaccga tcccaaaaga gaggctccca aggaagttag gggccagata aaccagggat
     3361 acggggacgt aaaaaagacg cccagcagac atgtgagaag agcccaatgt agcaaacggt
     3421 ataaccaata aatgcgtttc ttttttaata gtgaagcggg taaaagctca aaaaattctt
     3481 tcattccata ttcggcgccg agaacaaccg taacaaaaaa ccagccattc gctacgaggt
     3541 ccattgaacc cctcctttcc ctttaaagat aaacgatttc aattaacgtg tcaaggttaa
     3601 gtggcaaata ttaaccctct aaggttaata tttgaactca ttctcgccca cctacctctt
     3661 gctccgcgcg cttaataaag cgcgacctgc gagggaggag tagcctgatt gtgtcactgc
     3721 tacgactcac cctcgccctc tctttgcatc cgcaaaaaga gggtaacctc aaagctccga
     3781 actcaaaaaa cgcccccaaa caattgggca tttaaaaggg tgcacgccaa ctttgttggc
     3841 gcggtcgcct tccaaagaag gcgcgccctg ccggggcata aaaaagtttg aaatgtgaag
     3901 ggtgaagttt tcgggttttg tttcacactt cacaattcga agggacaacc tgggctatac
     3961 cggacatttc tttggcatct gtcacagggc tcaaggaagg agattacaat ctcatggcgt
     4021 atattcgaaa aaaagataaa aataaatagt atagcaaata atctataccg aggtatttta
     4081 gaatgaacca accttcatct tcccccaccc gctgtccctg gtgtctgggc caccccctgc
     4141 tcatcgaata tcacgacaag gaatggggca ccccagtcca cgatgagcgg caacattttg
     4201 aatttttgac tcttgaggca atgcaggccg gactgagctg gttcattgtg ctgcaaaagc
     4261 gggagatttt ccgtgaggct ttggaggact tcgattacca taagatagcc ggatatggcg
     4321 agaaggacat ggaacggatc atgggttttc cggggatcat ccgcaatcgc cggaaaatcg
     4381 aagcggccat ccataatgca cggatgtttc ttgcgatccg aaaggaattc ggctcttttg
     4441 acaactatat ctggggcttc gtcaatttta agccggttct ccattctttc aagacacaga
     4501 gcgaggtccc ggtgaccacc gaattgtccg accggatcag ccaggatctc aaaaaacgcg
     4561 gttttaaatt cctgggttcc gtcaccatct acgcccacct ggaagccatc ggggttgtga
     4621 atgaccactt agttagttgt ttcaggtacg gggggaaatg aatcgactca cccttgccct
     4681 ctctttgcat ccgcaaaaag agagtaaccc gaatgccctg aactccccta cctatcgctc
     4741 cctcatagga gtaaacacgg tatgtatcgc gcaaggtatt gtgttggtcg gttctcataa
     4801 gctacgcaat tattggaaag acggattcag aggcgaagat cagtcctacc agtcccggtc
     4861 cggtatgagc gcccaaagcc ggcgcgatca tgccggacgg caaccaatta catttataaa
     4921 gccgttccag tttttgtttt aataactcca ctccggcggc gttgttgcca tgaagtattt
     4981 gcaaacggag caggcttccc ggggtatgcc gctcctttac cagttccacc atctcgctaa
     5041 acgccgcggc catggtccga accttaccgc gttgcacata ggttccgcct tccttttcga
     5101 ccccaatgat cggcatgata tttaagattg aggcgagtaa tccctttaaa tgattgatcc
     5161 tgccgccgtg aatcaggtat ttcaaagacg gcaaagtaaa aatagtctca gtggcgtcac
     5221 taatctcctt aaccatcgtt aaaattcttt ctttggacca accggcctta acggctttag
     5281 cggcagcctc aacctgccaa ccttgcgctc cggaaagcgt cttcgtatcc accaacgtta
     5341 catccgcctc cgggaccgct gcagccccaa ttcgggcggc attgatcgtg ccgctcaggc
     5401 cggaagagat atggaaggaa agaatctcag catcggtctc cgccaggcgg cggtaaagcc
     5461 cagcaaactc tccggcggaa ggttgagagg tactgggaaa acttttggat gactctaaca
     5521 accggtaaaa ttcatctgat tggatatcaa ctccacaatg gtaggtttta ccgtctaaag
     5581 taaaagataa ggatacaaaa tgtatttcta atccattttt ttgggctgga gacagatcag
     5641 ccgcgcta
//
LOCUS       DASVMB010000270         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_622484,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000270 DASVMB010000000
VERSION     DASVMB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622484"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF531_07850"
     CDS             complement(<1..680)
                     /locus_tag="VF531_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7713924.1"
                     /translation="MIQVENVTKRYGQHVAVEQLNFTVNRGEILGFLGPNGAGKSTTM
                     NIITGYISATEGTVKVDGRDILEDPQAVKEKIGYLPEFPPLYPEMTVQEYLDFVCEIK
                     KVPETDRQENLERIVETVKIGEVKNRLIKNLSKGYKQRIGLAQALVGNPEVLILDEPT
                     VGLDPKQIIEIRNLIKELGKEHTIILSSHILPEVSAVCERVIIINKGKIVASDTPANL
                     AKSLAASS"
     gene            complement(982..2304)
                     /gene="melA"
                     /locus_tag="VF531_07855"
     CDS             complement(982..2304)
                     /gene="melA"
                     /locus_tag="VF531_07855"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX7713925.1"
                     /translation="MAKIAFIGAGSFGFTRGLVRDILSFPAFADSTIALMDIDTERLN
                     SIKKAVDRIVAEGHYPAKIIATTDRAEALRGADGVLCTILAGGIDIWRHDIEIPKKYG
                     VDINVGDTRGPAGIFRALRTIPVMLDICKDIERYCPDALFLNYTNPMAMLCRAMQGQS
                     KVKVTGLCHSVQGTAAMLAKWIGAPLDEISYLSAGINHQAWYLEYKWKGVDAYPLIRK
                     AILERPEIYNEEQVRNEMFLHLDYYVTESSGHNSEYNAWFRKRPDLIEKYCIHGTGWN
                     PGEYAYILNDYLKRETSWKQDIEKWLEEPVDLKRGQEYAASIFNAVFGDGTPFEFNGN
                     VRNFGLIDNLPVGCCVEVPVLASRRGLNSIHVGPLPEQLAILNNINARCEELAVEAAI
                     LGDPRKVFYAVYNDPLTSAVLSLDEIQRMVEEMLRVNAPYLPQFKQLT"
     gene            2482..>3090
                     /locus_tag="VF531_07860"
     CDS             2482..>3090
                     /locus_tag="VF531_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX7713926.1"
                     /translation="MADHFALEYQAAIDLSVYHCGTEKCAPGHAYGPAVRDHFLIHFV
                     HNGYGFFQAGGRTYHLGQGQGFLICPGIITYYQADASDPWHYSWVGFQGLKAKGYLET
                     AGLDEDHPVFRYDRDQQVDDYFRAMAAASGMQRGRELQLLGLLYQFLACLIDSREREG
                     TAGRGPDENINRRGLYLGKAQEFISRNYSRRITIGEIARFVGL"
BASE COUNT          720 a          797 c          799 g          774 t
ORIGIN      
        1 ttgctgctgg ccgccaaact cttcgcgaga ttggcagggg tatcgctagc cacaatcttt
       61 cccttattga tgatgatgac ccgttcgcaa actgcgctga cttcgggcag gatgtgagag
      121 ctaaggatga tcgtatgttc cttgcccaac tctttaatga gattgcggat ctcgataatc
      181 tgtttgggat ccaaaccgac ggtcggctca tccaggatca gcacctcggg gttgcccacc
      241 aaagcttggg ccaggccgat ccgttgtttg tatcccttgg aaaggttttt aatcagacgg
      301 ttttttacct cgccgatttt gaccgtctcc acgatccgct cgaggttttc ttgcctgtcg
      361 gtttcaggaa cttttttgat ctcgcataca aaatccagat actcctgaac ggtcatctcc
      421 gggtagagcg gcggaaactc cggtaaatac ccgattttct ccttcacagc ctgcggatcc
      481 tccaggatat cccggccgtc cactttgacc gtgccctccg tggccgagat ataaccggtg
      541 atgatgttca tcgtggtact cttcccggcg ccgttgggac caagaaatcc cagaatctcg
      601 ccgcggttca cggtgaaatt caattgttcc acggccacat gctgaccgta acgcttggta
      661 acgttttcta cctgaatcaa atattctccc tcccgtgtgc tttagtgtaa tcgcttagga
      721 tatttgcaat aaatatcaaa agtttgggat aatggtgaaa caatattcga tttaaaacca
      781 tcaaactcct ttgttacggt gggttttgag tccaagcacg cgtgatgtac accatgaacc
      841 gaacctcata acatattaca taacggagga agcggcaaat tagttgcagg tttttaccat
      901 ttaattagta tggcgttccc aacagaaatc caaaaagggg ctgtcctcag gacaacccct
      961 tttttaccat gctctggaaa atcaggttaa ttgtttgaat tgcggcagat atggggcatt
     1021 gacgcgaagc atctcctcga ccatcctctg aatttcgtcc aggctaagta ctgccgaggt
     1081 caaggggtcg ttgtaaaccg cgtaaaagac cttccgggga tctccaagaa tggccgcttc
     1141 cacggctaac tcctcacaac gggcgttgat attattcaga atcgccagct gttcgggtag
     1201 cgggcccaca tggatcgaat tcagtccgcg cctcgaggcc aaaaccggca cctctacgca
     1261 acagccaacg gggagattgt cgatcaaacc gaaattccgg acgttaccgt tgaattcaaa
     1321 gggagttccg tccccgaaga ccgcgttgaa aatagaggcg gcgtattcct ggccgcgttt
     1381 caggtcgacc ggctcctcaa gccatttttc gatatcctgt ttccagctgg tttcccgttt
     1441 taaataatcg ttgaggatat aagcatattc ccccggattc cagccggttc cgtggatgca
     1501 atacttctca atcagatcgg ggcgtttccg gaaccaggcg ttatactcgg aattatgtcc
     1561 gctggattcg gtgacgtaat aatccaggtg caggaacatc tcattgcgca cttgctcttc
     1621 attgtatatc tccggccgtt ccaggattgc cttgcgaatc aggggatagg catcgacgcc
     1681 tttccactta tactcaagat accaggcttg gtggttgatg ccggcgctaa gatagctgat
     1741 ctcgtcgagg ggcgcgccga tccatttggc cagcatcgcg gctgtcccct gcacgctatg
     1801 gcataagccg gtgaccttca ccttgctctg cccctgcatc gcccggcaga gcatggccat
     1861 ggggttggta tagttgagga aaagagcatc agggcagtaa cgttcgatat ccttgcaaat
     1921 atcaagcatc accgggatgg tgcgcagcgc ccggaagatt cctgccggac cccgggtatc
     1981 tccgacattg atatcgaccc cgtatttttt gggaatctcg atatcatggc gccagatatc
     2041 gatccctccg gccaagatgg tacagaggac gccgtcggcg cctcggagag cctcggcccg
     2101 gtcagtggtg gcaattatct tggcggggta atgaccttcc gccacgatcc ggtccacagc
     2161 ctttttgatc gaattgagcc gctcggtatc gatatccatc aaggcgatgg tactgtcggc
     2221 aaaggccggg aaagaaagga tgtcgcggac cagcccgcgg gtaaaaccaa aactacctgc
     2281 gccgataaag gcgattttag ccaatgaaac tactcctttc gtagaaaaga tgttgggaac
     2341 gatacttttg gcaagcgatt ccaatactca aattgtaaga tcaaacgggc aaaatagaaa
     2401 tggcaccatg tgatccaaat atggcatcat gttgtataat aaatataaac ccgcatatca
     2461 cggtaagtta caggaggagt catggcggat cactttgctt tggagtatca agcagccatt
     2521 gatctcagtg tgtaccattg cggcactgaa aaatgcgccc ccggacatgc ctatggtccg
     2581 gcggtccgcg accatttcct gattcatttt gttcataacg gatacggatt ctttcaagcc
     2641 ggagggcgaa cctaccatct cggccaggga cagggctttt tgatctgtcc cggaatcatc
     2701 acgtactatc aggccgatgc ctcagaccca tggcattact cctgggttgg ctttcagggc
     2761 ctgaaagcca agggttacct ggaaaccgcc ggactggacg aggatcatcc cgtctttcgt
     2821 tatgatcggg accagcaggt agatgactac ttccgggcga tggctgctgc atccggaatg
     2881 caacggggca gggagttgca gttgctcggt ttgctgtatc agtttttggc gtgcttgatt
     2941 gattcgaggg aacgggaggg taccgcggga cggggacccg atgaaaatat caaccggaga
     3001 gggctgtacc ttgggaaggc ccaggagttt atctccagga attattcccg gcggatcacc
     3061 atcggggaga tcgcccgctt tgtgggactt
//
LOCUS       DASVMB010000271         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_696643,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000271 DASVMB010000000
VERSION     DASVMB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696643"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..659)
                     /locus_tag="VF531_07865"
     CDS             complement(39..659)
                     /locus_tag="VF531_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713927.1"
                     /translation="MSFPAWAASAASPILRVGNDFSLSGPTILWKQASAMMLEEEMLL
                     DEGKQASLKVYLLYNDTSLLWGFDIQGLGPDYPDDYSPDFSGSDHVKLQFILRVKSQD
                     YPGSILILPDSIFQTPLVTPDYSYYQEHSGLPGYGIGAASIKEGEGVFIMLAIPYTAL
                     GSGIPAAGDSIDFKIGFVLAGSGQENWTLWVPTPGDSREYDTAKFE"
     gene            complement(766..1794)
                     /locus_tag="VF531_07870"
     CDS             complement(766..1794)
                     /locus_tag="VF531_07870"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7713928.1"
                     /translation="MIGVIGAGGWGTALAKFCSEKKYPVKLWVREPEVVAEINELHTN
                     HTFLPGVELPPEVVASNSLEEVISGNRFLITAVPSQWLRPVAKQMAALVDPQTIIVSV
                     TKGLEIQSLATMTTVLTEELPMLDPNAIVALSGPNHAEEVARQVPSATVVATPVLRYA
                     QEVQDLLISPLFRVYTNPDRTGIQLAGALKNVIALAAGISDGLGFGDNTKAALVTRGL
                     VEMARLGTAIGAQAPTFSGLAGIGDLFVTASSVHSRNSWAGRELGKGRSWAEISASTK
                     MVVEGVATAQAARQLAVRHGIEMPITEITYQILFENLSPREGVSILMERMRTHEMEEV
                     VAATYAWL"
     gene            complement(1791..2411)
                     /gene="plsY"
                     /locus_tag="VF531_07875"
     CDS             complement(1791..2411)
                     /gene="plsY"
                     /locus_tag="VF531_07875"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7713929.1"
                     /translation="MLLGIAILIGCYLLGSITTGDIVAAIKKVNLRNQGSGNIGATNA
                     FRTMGSVSGAIVLVGDALKGVIAVILGSLIPKFHGFDPAIIAGVLVIAGHNWPIFANF
                     RGGKGIATSLGVIIALTPLSLIVIIPAWIIVFLCFGYVSLASIMAAVFYPVGVYFFYQ
                     IDPYKIGFSILIAVLALYRHRSNFKRLATGEEHRILYQNRKGATKK"
     gene            complement(2430..>3064)
                     /locus_tag="VF531_07880"
     CDS             complement(2430..>3064)
                     /locus_tag="VF531_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7713930.1"
                     /translation="TAGIRRKSKVEESVEYYSVLRALRAMERSDVVILVVDATTNLTE
                     QDLKIAGLIKDAGKACIIGINKWDLVEKNEKTTEEYSKKIRNDLEFMDYAPLVFISAV
                     TGERLNRMMDLVDQVMANYSLAISTNHLNQLIGEATALHNPPSNKGRPFKIYYTQQVK
                     TKPPTFLFTVNDPDGFHFSYRRYLENRLRETFGFIGTPLRFQIRTGKAEK"
BASE COUNT          808 a          788 c          706 g          762 t
ORIGIN      
        1 accgtcacca caaaaattcc acgaaaatca aatttacctt actcaaattt ggcggtatca
       61 tattcccggg aatctcccgg ggtaggaacc catagcgtcc agttttcctg ccctgaaccc
      121 gcaagcacga acccgatctt gaaatcaatt gaatcgccgg ccgcgggaat accggaccca
      181 agggcagtat acggtatggc cagcatgatg aagactcctt caccctcttt aattgatgcc
      241 gcaccaattc catagcccgg aaggccggaa tgctcctgat agtaagaata atccggtgtc
      301 acaagcggcg tttggaagat actatcgggt agaataagga ttgatcccgg ataatcttgg
      361 cttttgaccc tcaggataaa ttgaagtttt acatgatcgc tcccggagaa atccggtgaa
      421 tagtcatcgg gatagtcagg gcccaacccc tggatatcaa atccccataa taaagaggtg
      481 tcattataga gtaaatagac ttttagacta gcctgtttcc cttcatcaag cagcatctct
      541 tcctcaagca tcatggccga ggcctgtttc cataatatgg ttggtccgga taacgaaaaa
      601 tcatttccga ctcgaaggat aggagaagcg gcacttgcag cccaggcagg gaatgacaaa
      661 ttgatacctg ccgttataat aagaattctt atgataacag tcaataaatg aaaggaatga
      721 tctcggcgtt tcatatcggt ccgttctcga tacatttata tccaattata accaggcata
      781 agtcgcagcc acaacttctt ccatctcatg ggtgcgcatc cgttccatta aaatgctcac
      841 cccttcgcgg ggggatagat tttcaaataa aatctggtac gtaatctcag tgataggcat
      901 ctcaattcca tgccgaaccg ccaattgccg ggctgcttgg gcagtggcaa ccccctccac
      961 caccattttg gtggaagcgg agatttcagc ccaactcctc ccttttccga gctcccggcc
     1021 ggcccaggaa tttctactgt gtacactgga agcggtaaca aacagatcgc caatcccggc
     1081 cagccccgag aatgtagggg cttgagctcc aatggccgtt cccaaacggg ccatctccac
     1141 caaacctctt gttacaagcg cagctttggt gttatctcca aatcccaatc catcggagat
     1201 ccctgccgct agagctatga catttttgag agcgccggcc agttgaatcc cggtgcgatc
     1261 cggattggtg taaaccctga acaagggcga aatgagtaaa tcttggacct cctgggcata
     1321 tcgaagtacc ggagtagcca cgacggttgc cgaaggaacc tgtcgtgcca cttcttctgc
     1381 gtgattaggg cctgacagag caacgatggc attcggatcg agcatgggca attcctcggt
     1441 gagtaccgtg gtcattgtgg caagactttg gatctcaagc cctttggtga cgctgacgat
     1501 gatggtttgg ggatcgacga gtgccgccat ttgtttggct accggacgta accattggct
     1561 tgggactgcg gtgattagga aacgatttcc cgagatcacc tcttccaggg agttacttgc
     1621 caccacttcc ggaggtagct ccactccggg aaggaaggta tgattggtat ggagttcatt
     1681 gatttctgca actacctccg gttcacgtac ccagagtttc accggatatt ttttttctga
     1741 gcagaatttg gccagcgccg taccccagcc gcccgcaccg atcacgccga tcattttttg
     1801 gtcgctccct ttctgttttg ataaaggatt cggtgttcct caccggttgc cagccgctta
     1861 aagttggacc ggtgccggta taaagccaaa acggcgatga ggatggaaaa accaatttta
     1921 taaggatcaa tttggtagaa gaaatatacc cccactggat aaaaaaccgc tgccatgatt
     1981 gaagctaacg agacatagcc aaagcaaaga aatacaatga tccatgcagg aattatcaca
     2041 atcaggctga gcggtgtcag ggcgatgatt acccccaacg aggtagcgat gccttttcca
     2101 ccacgaaaat tggcgaaaat gggccaatta tgtcctgcaa taaccagaac acccgcgatg
     2161 atagccggat caaacccatg gaattttggg ataagactcc ccaatataac tgcgattaca
     2221 cccttcaggg catcaccaac gagtacgatt gcccctgaaa cagaacccat ggtacgaaag
     2281 gcattggtcg cccctatgtt cccgctgccc tggttgcgga gattgacttt tttaatggct
     2341 gccacaatat ctccggtggt aatggaaccc aagagataac agccgattag gatagctata
     2401 cccaataaca tgttgtcgct cctgtaacct catttttcgg ccttacccgt ccgaatctgg
     2461 aaacgtaacg gggtaccgat gaaaccgaag gtttcacgta gtctgttctc aagatatctc
     2521 cggtaagaaa agtgaaaacc atcgggatcg ttcacagtaa aaagaaaagt gggcggtttg
     2581 gtcttcacct gttgggtata gtaaatttta aaaggccgtc ctttattgga aggagggtta
     2641 tgcaatgccg tcgcctcccc gatcagctgg ttcaaatggt tagtggatat tgcaagcgag
     2701 taattggcca ttacctgatc caccaaatcc atcatccggt tcaatctttc cccggtgaca
     2761 gccgaaatga agacaagcgg cgcatagtcc ataaactcaa gatcattccg gatttttttg
     2821 ctatattcct cagtggtttt ttcgttcttc tccaccaaat cccatttgtt gatcccgata
     2881 atacatgcct tacccgcatc tttaatgaga ccggcgatct ttaaatcctg ttcggtcagg
     2941 ttagttgtgg catccaccac cagaatgacc acatccgaac gttccattgc tcttagagct
     3001 cgaagcacgc tataatactc caccgactcc tcgaccttgg atttacggcg aatccccgcg
     3061 gtat
//
LOCUS       DASVMB010000272         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_696792,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000272 DASVMB010000000
VERSION     DASVMB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696792"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF531_07885"
     CDS             <1..630
                     /locus_tag="VF531_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713931.1"
                     /translation="GFSLCLQGLNGRHRVTLWWCLNFFIGLILVHPSFGADVGGGITA
                     LLGLGVANYLWLKRPIRIQEITRLVLITLGALALAGAWDLLVNRNSMSHLGQLLLSVR
                     NHGLVVFTTMVIRKISLNLRLISSTPLTLLLIGILLVVPVLYRFPPAALKRLKERHAE
                     AFAGLTGLAVTALIGFLVNDSGIVSAAMIFMFGIGLLLTVMIKELTGTN"
     gene            772..1953
                     /locus_tag="VF531_07890"
     CDS             772..1953
                     /locus_tag="VF531_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7713932.1"
                     /translation="MAKAIDILRQLLRKRLGLPAVILFVVIASLISSITVGSLLSQNY
                     NSEIKRKLQGMGQPANPLKGGDMLNAALIGNTASVVPIAQKAGPAIVGIRTTIRTSLE
                     RFFGPEAEQSRVEGSGVVLDSNGYIMTNYHVVQDADPRNVSRQSVTLEVFLSDKRQAK
                     AKFVGGDPLNDLAVIKIDLKNLPVAELGDSSKLQVGALAVAIGNPLGLEFAGSVTTGV
                     ISALNRTVSVEDRTLNLIQTDAAINPGNSGGARVDSEGKVVGINTVKISVSGVEGLGF
                     AIPINTAKPIVAQLVKYGYVKGRPFIGIGGQEITEEIARYYNLPIGIYVVSVAQGSGA
                     AKAGIHKRDILIGMAGKTVTSMQDLNTIKNDYKAGDTVKVTIVRGKKKIDLNLTFSEA
                     R"
     gene            1956..2750
                     /locus_tag="VF531_07895"
     CDS             1956..2750
                     /locus_tag="VF531_07895"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX7713933.1"
                     /translation="MAWVQDAAKGGTVSASVPVRFWEIDFLRGLAVCLMILLHLLDDL
                     YYFCGFKGIHPLAWMVWQRVTAGIFIILAGISLSLNRSRGETLGIKSPHWFLRHSLLR
                     GIKIFAWGMAVTLVTRAFLREGFVRFGVLHFLGLAMIAGIPFVKLRYENLWLGLFSLG
                     IGSYFARFQLKTAWLFWLGIRPRNFFSVDYFPFFPWFGLVLWGIFIGNTLYRDAERRF
                     DLHDYHNPAIRWGCFLGRNSLVLYLIHQPLFIAVLYSLGLIKGFRG"
     gene            2947..>3097
                     /locus_tag="VF531_07900"
     CDS             2947..>3097
                     /locus_tag="VF531_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7713934.1"
                     /translation="MALETRNRFWGTDFRTGFGQGIPIALGYIPVSFTFGLLAVKGGL
                     SPWIAV"
BASE COUNT          715 a          689 c          833 g          860 t
ORIGIN      
        1 ggtttctcac tatgtcttca aggcttaaat ggccggcacc gggtgacgct ttggtggtgt
       61 ttaaactttt ttattggttt aattctggtt catcccagtt ttggggctga tgtcggcgga
      121 gggataaccg cattgctggg attaggtgtg gccaattatt tatggctcaa acggcccatc
      181 cggatacagg agatcacacg gcttgttttg attacgctgg gtgccttggc tcttgccgga
      241 gcatgggatt tacttgttaa ccggaactcc atgagtcatt tgggacagtt gcttttatcc
      301 gtccgtaatc atggtctggt tgtttttaca accatggtga tccgaaaaat ctcactcaac
      361 ctgcgcttga taagcagtac tcccttaacg ctcttgctaa tcggaattct cctggtagtt
      421 cctgttttat accgatttcc acccgccgcg ctgaaaaggt tgaaggaaag gcatgccgaa
      481 gcgtttgccg gtttaaccgg gttagccgtg acggcactga ttggtttttt agtcaatgac
      541 tcagggatcg tttcggcggc catgattttt atgttcggta tcggtttgtt attaaccgtg
      601 atgatcaagg agttaaccgg tacgaattaa acgtttattt gcttatgagc ttattcaatg
      661 gttgctgctt caattcggtg attatggaga ccgggcgttg aacccgggat ttcataataa
      721 aaaatgaatc gggaaatcta ttctcaagag ttttcaggag ggaaaccgaa tatggctaaa
      781 gcaattgata tcctgcgtca acttttgaga aaaagactgg gcttgccggc ggttatactg
      841 ttcgtggtga ttgcctcgtt aatcagcagc attaccgtgg gcagcctgtt gtctcagaac
      901 tataattcgg aaataaagcg gaaactgcaa ggaatggggc aaccggccaa tcccttaaag
      961 ggcggggata tgttgaatgc cgctctgatc ggcaatacgg catcggtggt gccaatcgcc
     1021 caaaaagccg gtcctgccat tgttggaatc cgcaccacca ttcgcacctc cctcgaacgt
     1081 ttcttcggtc ctgaagcgga acagtccagg gtggaaggtt cgggagtcgt cctggactca
     1141 aatggctaca tcatgaccaa ctatcacgtg gtgcaagatg ccgatccccg gaacgtttcc
     1201 cggcagagcg tgaccctgga agtgttcttg tcggataaaa ggcaggccaa ggccaaattc
     1261 gtcggaggcg accccctaaa cgatctggcg gtaatcaaga tcgatctcaa aaatttgcct
     1321 gtcgccgaat tgggtgactc ttccaaactt caagtggggg cgctggcagt ggccattgga
     1381 aacccattgg gtttggaatt tgccggttcc gtaacgactg gggtgatcag tgccttaaac
     1441 cgtaccgtct ccgttgagga tcggaccctc aatctgattc aaaccgatgc agcgattaac
     1501 ccgggtaata gcggcggagc acgggtagat tcggagggaa aggtggtcgg aattaatacc
     1561 gtcaagatat cggtctccgg cgtcgaggga ctgggtttcg ccattcccat caacaccgcc
     1621 aaacctatcg ttgctcagct agtcaagtac ggttatgtca agggccgccc gtttattggc
     1681 atcggaggcc aggagataac cgaggagatt gccagatatt ataatctgcc gatcggaatt
     1741 tatgtggtct cggtggccca gggaagcggg gcggccaaag ccggaatcca taaacgggac
     1801 atcctcatcg gaatggcggg aaagacagta acctcgatgc aggatttaaa cacgatcaaa
     1861 aatgactaca aggccgggga tacggttaaa gtgaccatcg tccggggaaa aaagaagata
     1921 gacttgaatc tgacgttctc ggaagcccgg taaaaatggc atgggtgcaa gatgcagcaa
     1981 agggcggcac tgtctcggcg agcgtcccgg tacggttttg ggaaattgac tttctgcgag
     2041 gtttggcggt ttgcctgatg atactcctgc atctcctgga cgatctttac tatttttgcg
     2101 gtttcaaagg tattcacccg ctcgcttgga tggtttggca acgcgtcacg gcgggtatct
     2161 tcatcatctt ggcgggaatt tcattatcac taaatcgttc acgtggggag acgctcggga
     2221 tcaaatcacc ccattggttt ctccgtcata gcctgctacg agggataaag attttcgcgt
     2281 ggggcatggc tgtcaccctc gttacccggg cattcctcag ggaaggcttt gtccgtttcg
     2341 gggtgcttca cttcctgggg ttggccatga ttgccggcat tccatttgta aaattgcgct
     2401 atgagaacct gtggctcggg ttattttctt tgggaatcgg ttcctacttt gcccgatttc
     2461 aacttaagac cgcatggctg ttctggctgg ggataagacc ccgtaacttc ttttccgttg
     2521 attattttcc ttttttcccc tggttcggcc tggtgttatg ggggattttc ataggtaata
     2581 cgttataccg tgatgcggag cggagatttg atctacacga ttatcacaac cccgcaatcc
     2641 gctggggttg ttttttggga aggaattctt tggtgcttta tttgattcac caacccctgt
     2701 tcatcgccgt gctatacagc ttgggactga taaagggttt tcgcggttaa aatattgtat
     2761 aaaagaacgt ttttttgagt cacgaaactc acgaaacaca cgaaaagggg ttaaggcttt
     2821 cgtgaatttt cgtgttaacg gatatcaaaa tcattaatga aaccttactt gacaatgatg
     2881 gcgatatgta ttacttttta aaagtatagc aacatttggg tacagtggag gagcatcttg
     2941 gaagacatgg cgcttgagac cagaaaccgt ttttggggta cggatttccg taccggcttt
     3001 ggacagggga ttcccatcgc cctgggttac attccggttt cgttcacttt cggactgctt
     3061 gccgttaagg gcgggttatc accttggatc gcggtat
//
LOCUS       DASVMB010000273         8458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_33708,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000273 DASVMB010000000
VERSION     DASVMB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33708"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VF531_07905"
     CDS             <1..1306
                     /locus_tag="VF531_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX7713935.1"
                     /translation="FYFSAVFSLVCFLVLAVFYMHEFRAFLSVGVGIVLALVLDELTK
                     FFTDTHYKPVKDIAAASQTGSATLILRGLAVGFEASVWQVIVIAVTILAAVLIYWGQP
                     VIYVLYGVAMTGIGMLTLTGNNVAMDSFGPISDNANGIGEMAGLKEDARQIMADLDAV
                     GNTTKAITKGVAIGSAVIAAVSLFGSFLTDVGKVQAQMGLAENLRLSVTGIRVSEPNV
                     FIGMLIGAAIPWLFSSLAINAVARAAALIVDEVRRQFRIPGLMEGKVQPDYKQAVGIC
                     TAAAQRELISLALIAVVSPLVVGMLLRVEALGGFLAGVIVSGQLLAVFMSTSGGAWDN
                     AKKTIEDGIHGGKGSECHKASVVGDTVGDPLKDTAGPALNPMIKVINMVSLLAAPILI
                     QYKSNNPGVIVAVVICSVALVAGIMYSKRGGFKSKANTTAQF"
     gene            1382..2719
                     /locus_tag="VF531_07910"
     CDS             1382..2719
                     /locus_tag="VF531_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713936.1"
                     /translation="MEHIKNNRKNRWFYPLCRGRFRNSQIRFIFDGIAVNAALILTTG
                     PFLSGYIVLLKGSDFLAGLLNNSGTWASLISISFFLLYNRLDRPKRLLITLFLLSRIS
                     ICSIIFLPLFIKDKNLILSCMTALVVVGNLLWGFYSAGANVWLARILPRETRADYVFT
                     RMFWLRISFTLTTIVMGVLLDWFRKSYTGFLIVFLTSLVFSIIDLVILSGIQEPDAEE
                     IPRSGASFNDFLEPWSHPEYRHFLVFSLLFYLATSLANSFTPLYYIRYLKLSYSAISA
                     INVLTYLAMIFCTNFWGRIQRQQGSIYVLWITAIFIGSEFIFDSFLTTRTVFLLALAP
                     LLSGIGYSGFNTTTFNYRYEIMPERNQVIYEGWFGAMMGLGVLAGPVLGSWLMDQLPR
                     FQNRLYQFSQYQLLYLIAFCCTMLVILFFFRDSIRERLGAPKNNRNPGSSIPG"
     gene            complement(2964..4010)
                     /locus_tag="VF531_07915"
     CDS             complement(2964..4010)
                     /locus_tag="VF531_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX7713937.1"
                     /translation="METKAHGRFDFIDLLKGLGIFLVVWGHTMTPRSVFIYSFHMPLF
                     FFISGYLHRNKPIQEFFIGKIKRLLIPYALVTVLSWLFYLVVIIIQGNSEQLTIHLPK
                     IASLFTGSARNGGNDPIWFLTCLFVVSALFWLYQNFLKDDKRIAVVIAAGSLIGYLLG
                     AARILLPFKIDAAFSGLVFYFGGYLLRKRSDLTTLDKLGKPIVIITVALCLVAQFILA
                     RFNVALTGIPKVSMISNNLGNYFLFYITAVLGIAALLVFGYRVRTIPVYNSLGKHSLS
                     ILATHKPILFLLGELFDDILDIASPAYCFLGSIIAILINMSLVGLATLLGRYLPDFFG
                     TKNPSPLSGGVHGL"
     gene            4235..4789
                     /locus_tag="VF531_07920"
     CDS             4235..4789
                     /locus_tag="VF531_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713938.1"
                     /translation="MKKLIIFLILVAVLAAVGYWLQISYQIFDAPLAMVHEYLSRANQ
                     VYNGKLGAGITVGTVILIGVLLAAPFFTRKINTSMYFSNLKQGIISSFIFLLSQTIFN
                     YFNKFGSFYLLLAMLGVAIITLVLVRMAVRLFKNEKEGVEFRTSFISSVAAGLIFGVL
                     LNLGVVLVDLAKGKAAFLQATFFK"
     gene            complement(4882..6216)
                     /gene="gdhA"
                     /locus_tag="VF531_07925"
     CDS             complement(4882..6216)
                     /gene="gdhA"
                     /locus_tag="VF531_07925"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX7713939.1"
                     /translation="MSLTSELMKKVIKRNPGEPEFHQAVKEVLESLEPVLEKHPEYIK
                     AGIMERIVEPERQIIFRVPWVDDNGKVQVNRGFRIQFNSAIGPYKGGLRFHPSVYSGI
                     IKFLGFEQIFKNSLTGLPIGGAKGGSDFDPKGKSDTEVMRFCQSFMTELYRHIGADVD
                     VPAGDIGVGGREIGFLYGQYKKITGLYEGVLTGKGLTWGGSLVRTEATGYGLCYFVDE
                     MIKAKGKSFQGATVTVSGSGNVAIYATQKAQQLGAKVVAVSDSGGYVYDPEGIDVAIL
                     KQIKEVERKRISEYAKVRKSAQYHEGCSGIWTIKCDIALPCATQNEIDGNSANILVHN
                     GCWAVGEGANMPSTPEAIEVFLKNKVLFAPAKASNAGGVATSALEMSQNSMRYSWTFE
                     EVDAKLKQIMVNIYQNSSQAAKEYGHPDNLVVGANIAGFLKVAQAMLAQGIV"
     gene            6516..8060
                     /locus_tag="VF531_07930"
     CDS             6516..8060
                     /locus_tag="VF531_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7713940.1"
                     /translation="MPDDKSIIKGTALLTGAGFIVKLLGAAYRIPLARLIGAEGIGLY
                     QMAYPVYLIFLSLSTAGLPIAISRITADQIARGNFAGAKQVFHASLYLLSGLGLAGSL
                     AMALAARPLADKVVADPRAVFAMWAFAPAVFFMSLIAAFRGFFQGQQEMAPSAGSQVV
                     EQAVRVGVALILAVVLLGSGVEHAAAGAAFGATAGGAAGLVFLILIYWKRRVRFKRFS
                     ARRSGVRLGAVISRLIRFALPISVAVILMPLLQTLDSLIVPTKLQSIGYTVQESTSML
                     GILGNSWAVLYLPMIVTGAIASNLVPAITAALAGRARLEVKRRIHDGLRLAIIYLIPA
                     AAGTFLLGGTIYRIIYGVNHIDILSWLAPAILFLGIEQVSASVLQGLGKPKLPLGNFT
                     LGAVVKVIVTVVATGMPGLNLTGAALGTVCGSGLTAFLNLLAIKRLVPVGLSSVAKAS
                     LAGCGMIGIGWYLMRILYLHYLLEFCIVGLLSVLSYGLILWILGGITTADREVIAKLF
                     RKKETG"
     gene            8060..>8458
                     /locus_tag="VF531_07935"
     CDS             8060..>8458
                     /locus_tag="VF531_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEX7713941.1"
                     /translation="MAMELKDAGKSLSRLVEIMALLRSEQGCPWDRDQTHQSLKTCAV
                     EETYEVLEAIDSGNPDKLREELGDLLLQVVFHAQLAAEAGQFNIDDVVDAIVEKLIRR
                     HPHVFSDTHVSGVSGVLENWEKIKRQEMPEE"
BASE COUNT         1942 a         1981 c         2171 g         2364 t
ORIGIN      
        1 cttttacttc tcggctgttt tctccctggt ttgcttcctg gtgcttgccg tcttctatat
       61 gcatgaattc cgggccttcc tctcggtggg cgtcggtatt gttttggcgc tggttcttga
      121 tgaactgacc aaattcttca ctgatacgca ttataaaccg gtgaaggata tcgccgctgc
      181 ttcccagacc ggatcggcga ctttgatcct ccggggtctg gctgtcggtt ttgaggcttc
      241 ggtatggcaa gttattgtaa tcgctgtgac cattttggct gcagtgttga tttactgggg
      301 tcaaccggtt atttatgtac tttatggtgt ggcaatgacc ggtatcggta tgttaaccct
      361 aaccggtaac aacgtggcta tggactcctt cgggccgatc tccgataacg ccaacggtat
      421 cggtgagatg gccgggttga aggaagatgc ccgtcagatc atggccgatc tggatgccgt
      481 cggtaatacc accaaggcta ttaccaaggg tgtggccatc ggctccgcgg ttatcgcggc
      541 agtttccctc tttggttcct tcctgacgga tgtcggcaag gttcaagctc aaatgggctt
      601 agcggagaat ctccggctct ctgttaccgg aatccgggtt tccgagccga atgtcttcat
      661 cggcatgttg atcggtgccg cgattccttg gctcttctcc tctctggcca tcaacgcggt
      721 ggcccgggcc gctgctttga ttgtggatga ggtacgtcgg caattccgga tccccggcct
      781 gatggagggg aaagttcaac ccgattataa gcaggcggtt ggtatttgca ccgctgccgc
      841 acaacgcgaa ttgatctcac tcgccctgat cgccgttgtc tctccgctcg ttgtgggaat
      901 gctcctcagg gttgaggcgc tcggcgggtt cctggccggg gttatcgtct ccggacaatt
      961 gctggcggtc tttatgtcca cctccggcgg tgcctgggat aacgccaaga agaccattga
     1021 agatggtatt catggcggta aaggttccga atgccacaag gcgtcggttg tcggagacac
     1081 tgtcggtgat ccgttgaaag ataccgcggg tcccgctttg aacccgatga tcaaagttat
     1141 caacatggta tcgcttttag cggctcccat cctgattcag tataagtcca ataatccggg
     1201 tgtcattgtc gctgtcgtaa tttgctctgt ggctttggtt gccggaatta tgtattccaa
     1261 acgcggcggt ttcaagtcca aggcgaatac taccgcacag ttctaatcgt tcccgtatta
     1321 ccgcgaggcg ggggcatatg cccccgcctc tttaatcaaa actgcagttt tttaaaatag
     1381 aatggaacat ataaaaaaca accgaaaaaa tcgatggttt tatccattat gccgcggtag
     1441 attccgtaac agccagattc ggttcatctt tgacgggata gccgtaaatg cggcgctcat
     1501 cttgacgacg ggcccgtttc tctccggtta tatcgtttta ttaaaagggt cggactttct
     1561 ggcgggccta ttgaataatt cgggtacctg ggcttcgctg atcagcatct catttttttt
     1621 gctctacaac cgcctggaca gaccaaagag attattaatt acattatttc tcctctcgcg
     1681 gatatcgatt tgttccatca tttttttacc gttatttatc aaggataaga atcttatcct
     1741 ttcatgtatg actgccttgg tggtggtcgg gaatctttta tggggttttt actcagccgg
     1801 cgccaatgtc tggttggccc ggatcttgcc ccgggagacc cgggcggatt atgtttttac
     1861 ccgtatgttt tggctgcgga tctcttttac gctgaccacc atcgtcatgg gagtgcttct
     1921 ggattggttc cgcaagagtt ataccggatt cctgattgtc ttcctgacaa gcctcgtctt
     1981 ttccatcatt gaccttgtta tattatccgg gattcaggaa ccggatgctg aggagattcc
     2041 ccgatccggt gccagtttca atgattttct ggaaccgtgg agtcatccgg aataccggca
     2101 ttttctcgtc ttcagccttt tattctatct tgccacgtcg ctcgccaatt cctttacgcc
     2161 gttgtattac atccgttatt tgaaactgag ttatagcgcg atctcggcga tcaatgtcct
     2221 tacctatctg gcgatgatct tttgtaccaa cttttggggc aggattcagc ggcagcaagg
     2281 ttccatctat gtgctttgga ttaccgcgat ctttatcggc agcgagttta tctttgatag
     2341 ttttctcacg acaagaaccg tcttcttgtt ggctctggct ccgttgctat caggcattgg
     2401 ttacagtggt tttaacacga ccactttcaa ttatcgatac gaaattatgc ccgaacggaa
     2461 ccaggtgatt tatgaaggct ggttcggcgc aatgatgggg ttgggcgtcc ttgccggccc
     2521 ggtcttggga tcgtggctga tggatcagtt gccccgattc cagaatcggc tatatcagtt
     2581 cagtcagtac caattattgt atttgattgc cttctgttgt acaatgctgg tgatcctctt
     2641 tttctttcgc gattcgatcc gggagcgttt gggagcaccg aaaaacaacc gtaatccggg
     2701 gagttccatt ccgggatgat tttttttata ggttagtttt attaggggtt tccgttaacg
     2761 aggtttcaga gaaatgaaaa aattaaacat gaatgaacag tatgggtttg aaaaggtaga
     2821 tgaagggcgg tttatgagac gggagccccg caccccttaa tcttcttttt ttaacggaac
     2881 attgaaacaa aggagagtgt tggcctgaac ctacggcaat ggttcgggtt tttttgttgc
     2941 tttttaaatt tcgatttggc gtcctaaagt ccatgaactc caccggataa tggcgacggg
     3001 ttttttgtgc cgaagaagtc gggcagataa cggccgagga gagtagcgag gccaacaagg
     3061 gacatattaa taaggatcgc gatgatgcta ccaaggaaac agtaagccgg tgaggcgata
     3121 tcgaggatat cgtcgaaaag ttcgcccagc aggaagagta tgggcttgtg agtagccaag
     3181 atcgacagcg aatgctttcc gagagaatta taaaccggaa tcgtacgcac ccgatacccg
     3241 aagaccagta aagccgcaat acctaaaaca gcagtgatat agaataaaaa gtaatttcct
     3301 aaattattgc tgatcatgct gaccttgggg attccggtaa gggccacgtt gaagcgggcc
     3361 aggataaatt gagcgacgag acacagggca acggtgatta tcacgattgg tttaccgagc
     3421 ttgtcgaggg tagtcagatc gcttcgcttt cgaaggagat agcccccaaa ataaaagacc
     3481 agtcccgaaa aggccgcatc tatcttgaaa ggaagcaaga ttcgggcggc gcccaagaga
     3541 tatccgatta atgatcccgc ggcgatcacc acggcgattc ttttatcatc cttcaagaag
     3601 ttttgataca accaaaagag ggcactgacc acaaataaac aggtcagaaa ccagatggga
     3661 tcattcccgc cgttcctggc ggaaccggtg aaaagcgagg cgattttggg gagatgtatg
     3721 gttagttgtt ccgaatttcc ttggatgatg atgaccacca ggtagaataa ccaggaaaga
     3781 acggtgacaa gggcataggg aataagcaag cgtttgattt tccctataaa aaactcttga
     3841 atgggtttgt tccgatgcag atacccagag ataaagaaga aaagcggcat atgaaacgaa
     3901 tagataaaaa cggaacgggg agtcatggta tggccccaga ccaccagaaa gatgccgagt
     3961 cctttgagta gatcaatgaa gtcaaaccgg ccatgtgctt tggtttccaa ttctttgctg
     4021 cctctttaat cggttgttga atctttactt atttcgtccc cagttgttaa gcctccttta
     4081 cagacattga taaaattgag ttaattttta tgggtcacgg ttttgtaaag gagaaggaga
     4141 gaaatgacga taataaagat aatctcggga tgatgtaccc gggaccaaaa cggtcggttg
     4201 tcagtcgcta ccgtaaactg caaggaggct gcaaatgaaa aaactcatca tcttcctcat
     4261 cttagtggcg gttctcgccg cggtcggtta ttggctgcaa atcagttatc agatttttga
     4321 tgcacccctc gccatggttc acgagtattt atcaagggcc aatcaggtat ataatggaaa
     4381 gttgggtgcg ggtattactg ttggaacggt gattttgatc ggggtcttgc tggcggctcc
     4441 tttctttacc cgaaagatca atacttcgat gtacttttcc aacctaaaac agggtatcat
     4501 ttcctccttt atcttccttc tctctcaaac catatttaac tacttcaata aatttggctc
     4561 cttttattta ctcctggcta tgcttggagt ggcaatcatt acgctggtcc tggtacgaat
     4621 ggcagtccgc ctatttaaaa atgaaaaaga aggggttgag tttcgcacca gcttcatctc
     4681 cagtgtcgca gcagggttaa tctttggagt actgctgaat ctgggtgtgg tcctggtgga
     4741 tctggccaag ggaaaagcgg cttttcttca agcaactttt tttaagtgaa cgggtaagga
     4801 cattcttacg atcatcaata aaaaagcgcc tggctaaaaa ccaggcgctt tcattgtttg
     4861 cttcaggctt atctttgaat cttaaacaat accttgggcc aacatggctt gggcaacttt
     4921 caggaatccg gcgatattcg cgccgaccac cagattgtcg ggatgaccgt attcctttgc
     4981 cgcctggctg gagttttggt agatgttgac catgatctgc ttcaatttgg cgtcgacttc
     5041 ctcgaaggtc caggagtaac gcatgctgtt ttgggacatt tccagagccg aggtggccac
     5101 gccgccggcg ttggaagctt tggccggagc gaaaagcacc ttgttcttta ggaaaacctc
     5161 aatcgcctct ggagtggaag gcatatttgc accttcgccc acagcccagc aaccgttatg
     5221 caccaaaata ttggcggaat tcccgtcaat ctcgttttga gtagcgcagg ggagggcaat
     5281 gtcacatttg atcgtccata tgccgctgca accctcatgg tattgggcgg atttccggac
     5341 tttggcgtat tcgctgatcc gtttccgctc cacttcctta atctgcttca ggatcgccac
     5401 gtcgatacct tccggatcat agacataacc gccggagtca ctgacagcga cgacttttgc
     5461 ccccaattgc tgcgcttttt gggtggcata gatggcgacg tttcccgaac ctgagaccgt
     5521 tacggtggct ccctggaagg atttcccttt ggctttgatc atttcatcca cgaaatagca
     5581 gaggccgtat ccagtggctt cagtccgcac cagactgccg ccccaggtta agcccttacc
     5641 ggtcagtaca ccttcataga ggccggtgat cttcttatat tgaccatata aaaagccgat
     5701 ttcacggcca ccgacaccaa tgtcgcctgc aggcacatcg acatcggcac cgatgtgccg
     5761 gtataactcg gtcatgaaac tttggcaaaa gcgcatcact tcggtatcgg atttgccctt
     5821 gggatcaaaa tcgctgccgc ctttggctcc accaataggc aatccggtca gggagttctt
     5881 gaagatctgt tcaaaaccga ggaatttgat gatgccgctg taaaccgaag ggtggaaccg
     5941 taaaccgcct ttgtaaggcc cgatggcgct gttgaattga atacggaatc ccctgttgac
     6001 ctgcacctta ccgttgtcgt cgacccaagg aacccggaaa atgatctgac gttccggttc
     6061 gacaattcgt tccatgattc cggccttaat atactcggga tgcttctcta acaccggttc
     6121 gagggattcc agcacttcct tgacagcctg gtgaaattcg ggttcacccg gatttcgctt
     6181 aatgactttc ttcatgagtt cactcgttaa tgacatctaa aaaccgtcct ttccgcttgg
     6241 ggttatttaa tggaatacaa cgtaattaag taaaatcttg atttgaatag gagcagcaag
     6301 ctttcagcaa tgttgaaaaa tgattttgga atcatttttc acatgatgaa aatgattaaa
     6361 ttcattttat gcaatcccta ttccaaagtc aatagaaacg gatcatgtat catgcataca
     6421 ttgggcccaa atctttatca tgaaaattat tattggggcg tatcttgtat ggcgagtcgt
     6481 ttcatattca atgatacctt tctaaggtga ttcaaatgcc ggatgataaa tcgataatca
     6541 aagggaccgc gttgttgacc ggtgccgggt tcatcgttaa attattgggc gcggcttacc
     6601 ggattcccct cgcccggtta attggcgctg aaggtattgg cctttaccaa atggcgtatc
     6661 cggtttatct aatctttctc tcgctttcca ccgccgggtt gcccatcgcc atctccagaa
     6721 taaccgcgga tcagattgcc agaggtaact ttgccggagc caagcaggtt tttcatgcct
     6781 cgctctattt attaagtggt ttaggcctgg ccggctccct ggctatggct ctagccgccc
     6841 ggcctctcgc cgacaaagtg gtggccgatc cgcgggctgt cttcgcgatg tgggcctttg
     6901 ccccggccgt tttttttatg agccttattg ccgctttccg tggatttttt caaggacagc
     6961 aagagatggc ccccagcgcc ggttcacaag tagttgaaca ggctgtcagg gtgggggtgg
     7021 cgctgatcct ggcagtcgtc ttacttggat caggagtgga gcacgccgca gcaggggccg
     7081 cgttcggagc gacagccggc ggcgcggcag gccttgtttt tctcatctta atctattgga
     7141 agagacgagt tcggtttaaa agattttccg ccagaagatc cggagtccga cttggggccg
     7201 tcatcagccg attgatccgt ttcgcgcttc ccatatccgt ggcggtcatc ttgatgcctt
     7261 tactacagac cctcgattcc ctaatcgttc ccacaaaact tcaaagcatc ggctatacag
     7321 tacaggagtc cacatcgatg ctgggtattt tgggcaattc ctgggcagta ttatatctgc
     7381 cgatgatcgt caccggagca atcgcatcca acttggttcc ggccataacc gcggccctgg
     7441 ccggcagggc tcgattggaa gttaaacgaa ggattcatga cgggctgcgc ttggccatca
     7501 tctatttaat tcccgcggct gcgggtactt ttctgttagg cgggacgatc taccggatca
     7561 tttatggcgt taatcatatc gacattttaa gctggttggc tccggctata ttattcctcg
     7621 ggattgagca agtatccgca tccgtattgc aagggttggg taaacccaaa ctgcctctgg
     7681 gtaattttac actgggggca gtggtcaagg taatcgttac cgtggtggct acggggatgc
     7741 ccggactaaa tttaaccggc gcggcattgg gaacagtctg cggttccggg ctgaccgcat
     7801 tcttgaattt actggctatc aaacgtctgg tcccggtagg tctatcatct gtagccaagg
     7861 catcgcttgc gggatgcggt atgattggta tcggctggta cctgatgcgt atcctttacc
     7921 tgcattacct gttggaattc tgtattgtcg gtctgttgag tgtccttagt tatgggttga
     7981 tcttatggat cctgggagga atcactacgg cggatcgcga agtaatcgct aaattattcc
     8041 gaaaaaagga gactggctga tggcgatgga gttgaaagat gcgggaaaga gtctatcccg
     8101 tctggtcgag atcatggcgc ttttacgcag tgaacagggt tgcccgtggg acagggatca
     8161 aacccatcaa tctttaaaga cctgtgcggt tgaagaaacc tatgaagtct tggaagcgat
     8221 tgattccggt aatccggaca agctccgcga ggagctgggg gatctgctgc tccaggtggt
     8281 ttttcatgcc cagttggctg cagaggccgg ccagttcaac atcgacgacg tggttgacgc
     8341 aattgtcgaa aagttaatcc ggcggcatcc gcacgtattt tccgatacac atgtttccgg
     8401 tgtttccggg gttcttgaaa attgggagaa gatcaaacgg caggagatgc cggaggag
//
LOCUS       DASVMB010000274         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_255685,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000274 DASVMB010000000
VERSION     DASVMB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255685"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VF531_07940"
     CDS             complement(<1..539)
                     /locus_tag="VF531_07940"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7713942.1"
                     /translation="MINSSVPNTALHSLPESGREPEGTVNAVIRALSILDLLGQANEE
                     LGVTEISREIGLHKSTVFRLLNTLALSGYVKQNPKSGKYSLGIHLVQLGMSVLNQIDL
                     RRVARPYLEELLHTCGEAVHLGILDQGEVIYIDKIDVERPLTMGSRIGGKSPGYCTGL
                     GKVLMAYLPEPELQLLVAKG"
     gene            1019..>1616
                     /locus_tag="VF531_07945"
     CDS             1019..>1616
                     /locus_tag="VF531_07945"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7713943.1"
                     /translation="MKGVFDELDTPNLIVDRGILEANLERMGNLAASKGVKLRPHAKT
                     HKCVEIGRMQLKYGAGGLTLAKTSEAMNFAEQGFNDIFLAYPVVGPVKLRRLIDLSHK
                     CRVTVGVDSLEGAAELNRAFESDRSKLPVLLEVDTGLGRTGIGLDKVRSLAEGVSRMA
                     HLEINGIYTFRGALLSEIQDSSDAGIKAAVDKQGRAEAE"
BASE COUNT          454 a          339 c          398 g          425 t
ORIGIN      
        1 cccttggcca ccaaaagctg aagttcgggt tcggggagat aagccatgag gactttaccc
       61 aagccggtgc aatatcccgg agatttgccg ccgatacggg aacccatggt taacggacgc
      121 tccacatcaa ttttatcgat atagatgact tcgccttgat caaggatccc gaggtgtact
      181 gcttcaccac aggtatgcaa caattcttca agatacggcc gggctacccg gcgcaggtca
      241 atctggttca gaacactcat gcccaactgt accagatgga tgcctaaaga atacttaccg
      301 cttttcgggt tttgcttaac gtaaccgctg agcgccaagg tgttcaaaag ccggaaaaca
      361 gtgcttttat gtaaaccgat ctcccggctg atctcggtga ctccaagctc ttcgttggct
      421 tgtcctaata aatccaggat tgacagggcc cggattacgg cattcaccgt tccttccggt
      481 tcgcggcccg attccggaag agagtggagc gcagtatttg ggacagatga atttatcaca
      541 agcaacacct tccgtataaa agaattttct gtattggttt gatccctaaa aggctaaatc
      601 gtgcaagccg gataaaaatg tcctaaagaa aaaatttttg ttaaaaaggg aagcaggatt
      661 ttataattcc atgttgaagc aatttattgt tcaaaaagtg aaatgatgtt tcacgatgcg
      721 aaacaacatg agaatttgta aatgatcttt gaatgaattg taatataatg catcgatgat
      781 ttctttaatc ataacgcaaa atgggtataa atgcaagtta agtcaagcca agaaggcatt
      841 ggaccttttt taaaaaaatt gggaatggat ttcatgccgg gattttttga aacgatgttt
      901 caccatgtga aacaaaatcc gagtaattct ttccattaat aattctataa tttcctgtga
      961 gcatcattaa gttctgtacg attgtctact atactaaaca atttacagga ggatgactat
     1021 gaaaggtgta ttcgatgaat tggatactcc caacctgatt gttgaccgcg gtattctcga
     1081 ggccaatctg gaacggatgg gaaacctggc cgcgtccaaa ggggttaaac ttcgccctca
     1141 cgcaaaaacg cataaatgcg tggaaattgg gcgaatgcaa ttaaagtatg gagccggcgg
     1201 attgactctt gccaagactt ctgaggcaat gaactttgcc gagcaggggt tcaatgatat
     1261 cttccttgcc tatccggtag tgggcccggt caagctcagg cggttgattg atctatccca
     1321 taaatgccgg gtaacggtgg gagtagatag ccttgagggg gcggcagaac taaatcgggc
     1381 ttttgaatcc gatcgttcga aattaccggt tttattggaa gtcgataccg gactcggacg
     1441 taccggcatt gggcttgata aagtcaggtc ccttgccgaa ggggtatccc ggatggctca
     1501 tttggagatt aacgggatat acacatttcg gggggcattg ctctctgaga ttcaagattc
     1561 cagcgatgcc gggatcaagg cggccgttga taagcagggc cgtgccgagg cagaaa
//
LOCUS       DASVMB010000275         6007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_33965,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000275 DASVMB010000000
VERSION     DASVMB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33965"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1074)
                     /gene="prfA"
                     /locus_tag="VF531_07950"
     CDS             complement(4..1074)
                     /gene="prfA"
                     /locus_tag="VF531_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7713944.1"
                     /translation="MFDKLAAIEEKYNELNNELSDPKVIADQSLFQKLAKAQAALSEL
                     VETFQEYKRITADIETAVAMLEEESGEDGELLGDELKKLRVEQERLSNRLKVLLLPKD
                     PNDEKNVIIEIRAGTGGEEAALFAGDLFRMYSRYAEARNWRLELLSTSESDLGGFKEI
                     IFNITGGQVYSRMKFESGVHRVQRVPETEASGRIHTSAATVAVLIEAEEVDVSINPND
                     LRIDVYRAGGHGGQCVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKDKAMKVLRARLL
                     AKFRNEQQQEMAQARRDMVGSGDRSERVRTYNFPQNRLTDHRINLTLYHLDSIIEGNL
                     DQVIEPLITADQADRLEHIDEA"
     gene            complement(1180..2145)
                     /locus_tag="VF531_07955"
     CDS             complement(1180..2145)
                     /locus_tag="VF531_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX7713945.1"
                     /translation="MKQQPEFQYGGQAVIEGVMMRGRDYLAVAVRKADGEIVIQKTPV
                     GSITKKFPFLKWPFIRGIVGLCESFGVGMKALMFSADQFMDDGETNEKGEPNKLSGWE
                     TALTVTLALALTIVLFIILPLFGRSLMGKILPGPVWGNIIESILRMIILVGYIVAVSS
                     MNDIKRVFAYHGAEHKVINTFEAKEDLTVENARKHTTFHPRCGTSFLLFVVIVSAVFF
                     SFLHYDNALMRFASRIALLPIIAGVSYEIIKFTGRHQDFFLWRWLSAPGFWLQRLTTR
                     EPDDDQLQVAISSLVAVLREEAPIIVGGKIYTAEKVEVADSATTS"
     gene            complement(2247..2447)
                     /gene="rpmE"
                     /locus_tag="VF531_07960"
     CDS             complement(2247..2447)
                     /gene="rpmE"
                     /locus_tag="VF531_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX7713946.1"
                     /translation="MREGIHPQFFKTKVTCACGETFEAGSTKKELRVEICSKCHPFFT
                     GKQRIVDSGGQVEKFKKRLEKK"
     gene            complement(2537..3793)
                     /locus_tag="VF531_07965"
     CDS             complement(2537..3793)
                     /locus_tag="VF531_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7713947.1"
                     /translation="MSGSKIHAIYADPRGKLQVDSDLIAVGLNGDQVEPADATWIDLP
                     AGGDLMLLPGRLPLGFNENSGALEVAEASEAGPVTAVAAILPVGLTRCLLPGYEIDDY
                     QELPLFGYTAVGSDGDRLKVAAVRTDADLKWNPVYYNTPDLPGLIAKKRGEFPENRIL
                     RQLARCAEEYHCLTAQNIFYERWEAGIPVSPVCNASCMGCISRQPAECCPAPQSRIKF
                     IPTISEVVSLAAGHLERAGDAIVSFGQGCEGEPSLQADLLEPTIRSIRERTSSGTINI
                     NSNAGATGAIRRLVDAGLDGIRVSLFSARPENYCWYHQPSGYDLAAVEHSAAYAAEHG
                     VNVALNLLLYPGFTNHPAETEALYGFLTRTGVRQIQLRNLNLDPEWLTEKLTSGPLPG
                     ITEWLDRLKENFPGLAVGNYTQPRSG"
     gene            complement(3901..5076)
                     /locus_tag="VF531_07970"
     CDS             complement(3901..5076)
                     /locus_tag="VF531_07970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX7713948.1"
                     /translation="MNQVILKSGKDKRIKAGHLWVYQGEIGIIGIGVKSGEVVEVLDN
                     RGRFLGIGYYNQASQIAVRLLTTSRENINEDFFRRRLEQALSFRKRVLSKETSCYRVV
                     FGEADLLPGLIVDRFQEYLVVQFLTMGMEVNRELILKLLVELCGPRGIIERSDLSVRH
                     LEELPERSGCVYGDCPGSVVIRDNGLQFRVNLLEGQKTGYFLDQSANRAVLQEYAGGR
                     RTLDCFCHLGSFAIHAAAYGAKEVLGIDISEDAIELAEENASLNGLGEKCEFKVANAF
                     DFLRDQAGKKELYDLIILDPPAFTKSKQTLDSAIRGYKEINLRAMKLLPPGGILVTCS
                     CSHHLTPDLFWEVINAAAADNKRRIRLLERRTQNLDHPVLVGVPETEYLKCYIFEMM"
     gene            complement(5115..>6007)
                     /locus_tag="VF531_07975"
     CDS             complement(5115..>6007)
                     /locus_tag="VF531_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7713949.1"
                     /translation="GVAIFIREEAEEITVQEGRVRGIRTSGGQEYLSEYVIVGPGREG
                     SDWLVKEARRLKLDSVVNPVDIGVRVELPAEVLEELTSVIFETKFIYYSRTFEDRVRT
                     FCMCPHGEVVMENSDGLVTVNGHSHAERKTANTNFALLVSKNFTHPFREPITYGRYVA
                     GLANLLGGGVIVQRLGDLQSGRRSTKERINKGLVRPTLAEDTPGDLSLVFPYRHLLAL
                     FEMLKALDTIAPGVNSRNTLLYGVEVKFYSSRLSLNQHLETEIPNLYAVGDGAGVTRG
                     LMQASISGLIAGRDILGKAK"
BASE COUNT         1466 a         1750 c         1384 g         1407 t
ORIGIN      
        1 tttttacgct tcatcgatat gctccagccg gtcggcttga tcggccgtga tcaagggctc
       61 gatcacctga tccaggttcc cttcgatgat cgaatccaaa tggtaaaggg ttaagttgat
      121 ccggtgatcc gtcaggcgat tttggggaaa attataggtg cggacccgct cgctacggtc
      181 cccggaaccc accatatccc ggcgggcctg ggccatctct tgttgctgct cattccggaa
      241 tttggccaac aaccgcgctc ttaatacctt catcgcctta tccttatttt ttaactgcga
      301 cttctcatcc tgacaggtca caaccaatcc ggtgggcaga tgcgtaatcc gtaccgccga
      361 atcggtggta tttacgcatt ggccgccatg acccccggcg cggtagacat cgatccgcaa
      421 gtcattcggg ttgatcgaca catccacctc ttcagcctcg atcaatacgg caaccgtagc
      481 ggcggaagta tgaatcctgc cgctcgcctc ggtttcaggt acgcgctgca cccggtggac
      541 accgctttca aacttcatcc tgctataaac ctgtcccccg gtaatgttga agatgatttc
      601 cttaaaaccg ccgagatcgg actcactggt acttaacagt tccagacgcc aattccgtgc
      661 ctccgcgtaa cgagagtaca tccgaaataa atcaccggcg aaaagggccg cctcctcccc
      721 gccggtaccg gcgcggatct cgataatcac gttcttttca tcgttagggt ctttgggaag
      781 caacaatacc ttgagccggt tactcaaacg ttcttgttcc acccggagtt ttttcagctc
      841 atcgccgagc aactccccgt cctcgcccga ctcctcctca agcatcgcca cagcggtctc
      901 gatatccgca gttatccgtt tatactcctg aaaggtctcc accaattcac tcagggcggc
      961 ttgggccttg gcgagctttt gaaacaagga ttgatcggcg atgactttgg ggtcgctcaa
     1021 ttcgttattg agctcgttat acttttcctc aattgccgcc aatttgtcaa acatgtaacc
     1081 acctgtaccc taaaaggttt cccgtaacaa ccgtagtcta ctcagcccat cggaaacgag
     1141 ggctgtaatg atgaattcct gctatcggaa ctcccgcctt tagctggtcg tagccgagtc
     1201 ggccacttca actttttcgg cggtataaat cttgccgcca acgatgatcg gagcctcctc
     1261 acggagcacc gccactaaag agctgattgc cacctgtagc tgatcatcat ccggctcccg
     1321 agtggttaaa cgttgcagcc aaaaaccagg ggcactcaac caacgccata aaaagaaatc
     1381 ctgatgcctt ccggtgaact tgataatctc ataagaaaca ccggcgatga tcggaagtaa
     1441 ggcaatccga gaagcaaaac gcatcaaggc gttgtcgtaa tgcaaaaaag agaaaaagac
     1501 cgcgctgacg atgaccacaa acaacagaaa gctggttccg caacggggat gaaaggtagt
     1561 atgctttctg gcattttcca ccgtcaaatc ctctttggct tcgaaggtgt taatcacctt
     1621 atgctcggcc ccgtgatagg caaaaacacg tttgatgtcg ttcatggaag aaacggccac
     1681 gatataacct accaggatta tcatacggag gatactttcg ataatgttac cccaaaccgg
     1741 tcccggcagg attttgccca tcagtgaccg cccgaaaagc ggcagaataa tgaagagcac
     1801 gatggtgagg gccagagcga gcgtaaccgt taatgcggtc tcccaacccg acaatttatt
     1861 gggttcgccc ttctcattcg tctccccgtc atccatgaac tgatccgccg agaacatcaa
     1921 ggccttcata ccgacgccaa aagattcaca aagccctact atcccacgga taaatggcca
     1981 ttttaaaaac ggaaacttct tggtgatcga gccgacagga gtcttttgaa tcacgatctc
     2041 cccgtccgct ttacggacag cgaccgccag gtaatcacgg ccgcgcatca tgaccccttc
     2101 aatcaccgcc tgcccgccat attggaactc gggctgctgt ttcataaatt acaccctctt
     2161 atattcgttt aaaggcgagg attcattacc cgattcctcg cctttacaga ttgccttctt
     2221 ttgaattgaa aaagctaacc ttaatcttac ttcttctcaa ggcgtttctt aaacttttcc
     2281 acctggccgc cgctgtccac gatccgttgt ttacctgtaa aaaacgggtg gcactttgaa
     2341 catatttcaa cccggagttc ctttttggtg gaaccggcct caaaagtttc cccgcaggcg
     2401 caagttacct tggttttaaa gaattgcgga tggattcctt ccctcatttc gtttcacctc
     2461 acctacagta tccattaact tttatattat atcacagagg attttagtct gcaataatat
     2521 tatccgatag gtaaattcaa ccggatcggg gttgggtata attacccact gccagtccgg
     2581 ggaagttttc tttcagcctg tccagccact cggtaatccc cggcagcggt ccagaggtta
     2641 atttttcggt gagccattcc ggatcaagat tcagattgcg aagttggatc tgtcggacgc
     2701 cggttctggt caaaaagccg tacaacgcct cggtttcagc gggatgattg gtaaagccgg
     2761 gatataacag tagatttaac gccacgttta ccccatgttc cgcggcataa gccgccgaat
     2821 gttccacagc cgccagatca tagccggaag gttgatgata ccaacaatag ttctccggcc
     2881 gggctgaaaa aagactaacc cggataccat ccagtccggc gtcgaccaaa cggcgaatgg
     2941 ccccggtggc cccggcgttg ctattgatat taatggttcc acttgaggta cgctcacgga
     3001 tggaccggat tgtaggctcc agtaaatccg cctgaagtga tggttcaccc tcgcagccct
     3061 gaccgaaact gacgatggca tccccagccc gttccaggtg gccggcagcc agagaaacta
     3121 cctcagagat ggtgggaatg aacttgatcc ggctctgcgg cgccgggcaa cactccgccg
     3181 gctggcgtga gatgcaaccc atacaggagg cattgcaaac aggcgatacc ggaatcccgg
     3241 cttcccaacg ttcatagaag atattctggg cagtcagaca atgatattct tcagcgcagc
     3301 gggccagttg ccgcaggata cggttttcgg gaaactctcc gcgcttcttt gcgatcagac
     3361 cgggcagatc gggagtgtta taataaacgg gattccactt gagatcggca tcggtccgca
     3421 cggcagccac cttaagcctg tcaccgtcgg acccgaccgc ggtatatcca aagagaggta
     3481 actcttggta atcgtcaatc tcataacccg gaagcaaaca ccgggtcaaa cccaccggca
     3541 atatggcggc caccgcggtt accggaccgg cttccgaagc ctccgctacc tctaaggctc
     3601 ccgaattttc attaaatccc agcggcaacc ttccgggaag caacatcaaa tcgcctcccg
     3661 ccggtagatc gatccaggtg gcgtcggccg gttcgacctg atctccattc aaaccaacgg
     3721 cgatcaaatc cgaatcaacc tgcagtttac cccgggggtc ggcgtaaatg gcatgaatct
     3781 tggaaccact catcaatcca ggcactcatt tccttggtga tctctcgaac ctcgattcgg
     3841 caagtccgca acgttactta ccggataccc gttcatgttc tatcgctcgg caaaacttta
     3901 ttacatcatc tcaaagatgt aacactttag atactcagtc tccgggaccc cgaccaaaac
     3961 cggatgatcc agattttgag tccttcgttc caaaagccga atccgtcgct tattatcggc
     4021 ggcggctgca ttgatgacct cccagaagag atcgggggtc aaatgatgcg agcaagagca
     4081 ggttaccaaa atcccaccgg gcggcaataa cttcattgcc cgtaaattga tctccttgta
     4141 accccgaatg gcgctatcca gcgtctgttt acttttggta aaagccggag gatcaagaat
     4201 gatcagatcg taaagctcct ttttgccggc ctgatccctt aagaaatcaa aagcgttggc
     4261 caccttgaac tcacacttct cgcccagccc gttcagactg gcattctcct ccgccaactc
     4321 tatcgcatcc tcggagatat caattcccaa tacttccttg gccccgtaag ccgccgcatg
     4381 tatggcaaag ctgcccagat ggcaaaaaca gtccaaggta cgccggcccc cggcgtattc
     4441 ctgcaacacg gcccggttag ccgattgatc cagaaaatat ccggtctttt gaccttccag
     4501 cagattcacc cggaattgaa gcccgttatc cctgatgacc accgatccgg gacaatcacc
     4561 gtaaacacat ccggagcgct ccggaagctc ctccagatgc cggacggaaa gatcgctccg
     4621 ttcgatgatt ccgcgaggcc cgcagagttc caccagaagt ttgaggatga gctcacggtt
     4681 gacttccatt cccatggtca aaaattgaac taccaaatac tcctgaaagc ggtccacaat
     4741 gagtccgggc aagagatcag cctcaccgaa aacaacccgg tagcagctgg tttccttgga
     4801 aaggacccgt ttacgaaaag acaaagcttg ctccagacgt ctccggaaaa aatcctcatt
     4861 gatgttttca cggcttgtgg tcaaaagacg caccgctatc tgtgaagcct ggttgtaata
     4921 gccaatcccc aggaaccggc cccggttgtc caagacctca accacctcac cggatttgac
     4981 gccgatcccg atgatgccga tctcgccttg atatacccag agatgacccg ctttgattcg
     5041 tttatcttta ccgctcttca atatcacttg attcatggaa acctcggtta acattagtta
     5101 ttggttatta gcttttactt tgccttaccc agaatatccc tgccggcgat cagtccagag
     5161 atcgaggcct gcatcaagcc ccgggttacg ccggcaccat cacccaccgc gtaaagattg
     5221 gggatttccg tctcgaggtg ttgattgaga cttagccggg atgagtagaa cttgacttct
     5281 accccataaa gcaaagtgtt gcgggagttg acccccggag caatggtatc taatgctttc
     5341 aacatctcaa ataacgccag tagatgacgg tacggaaata ccaggctcaa atcacccggg
     5401 gtgtcttcgg ccaaagtcgg acgaaccagg cccttattga tccgctcctt ggtggagcgc
     5461 cggccgcttt gcaaatcccc gagccgctgc acgatcaccc cgccgcccaa taaattggcg
     5521 agacccgcca catacctgcc gtaagtgatc ggttcccgga aaggatgagt aaagttcttg
     5581 ctgacaagca aggcaaagtt agtattggca gttttccgtt cggcgtgact gtgcccgttt
     5641 accgtaacca gcccgtcgct attttccatg actacttcac catgggggca catgcagaag
     5701 gtccggaccc ggtcctcaaa agtacgcgaa taatagatga acttggtttc aaaaatgacg
     5761 ctggtcagtt cctcgagtac ttcagccggg agttccaccc ggaccccgat atccaccgga
     5821 ttgaccaccg agtcgagttt caaccgccga gcctccttga ccaaccaatc cgaaccttcc
     5881 ctgccgggac cgacgatcac atattcgctc aggtactctt ggcccccaga ggtacgaatc
     5941 cccctgactc tcccctcctg gacagtgatc tcttccgcct cctcgcggat gaagatcgcc
     6001 acaccct
//
LOCUS       DASVMB010000276         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_400840,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000276 DASVMB010000000
VERSION     DASVMB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400840"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF531_07980"
     CDS             complement(<1..356)
                     /locus_tag="VF531_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEX7713950.1"
                     /translation="MKLVETKVVNLREIHETAIVHPSAKIGRNVVIGPYAVIGENVEI
                     GDNCVIGPQVMIDGWTTIGSGNKFYHGASIGCEPQDLKFKGEKSFLVIGDDNMFRENV
                     TISRGTEGGGGETRIG"
     gene            complement(755..1180)
                     /gene="fabZ"
                     /locus_tag="VF531_07985"
     CDS             complement(755..1180)
                     /gene="fabZ"
                     /locus_tag="VF531_07985"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018661155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7713951.1"
                     /translation="MIDINEIKKMLPHRYPFLLVDRVLEIEPEKRVVALKNVTANENF
                     FNGHFPSKPVMPGVLIVECMAQAAGLVMLSEEKHKGKVPYFTGIDKARFRRTIVPGDQ
                     IIIEVEVLHLRGNVGRVQAFAKVDNQIAAEAEMMFILGE"
     gene            complement(1271..2110)
                     /gene="lpxC"
                     /locus_tag="VF531_07990"
     CDS             complement(1271..2110)
                     /gene="lpxC"
                     /locus_tag="VF531_07990"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7713952.1"
                     /translation="MEFQQTVAAAFDITGQALHSGAMVRMRITPQPENTGIWFGRSDL
                     PGSPKIKAEPSAVVDTKKSMTLGRDGWRIATIEHLMAVFHGLGVDNVLVEVDGAELPR
                     GDCSGLFFANEFLRVGLHKQSEPRNYYCIKEPVWVEGVVPRGNQPLKAWIIALPSDEF
                     RVSFTFTSDHPVTGTQYYQYQHTPENFMSDVAPARTIAFRREIEYLQSQGLALGGDID
                     SVVVVGDHGYESELRFPEEIVRHKILDILGDLYLVGPLRGHIIAVRSGHTLDFELARK
                     MAK"
     gene            complement(2348..3076)
                     /locus_tag="VF531_07995"
     CDS             complement(2348..3076)
                     /locus_tag="VF531_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7713953.1"
                     /translation="MLKISGLMKSYGRKQALNGVDLAVDRGETVVIMGPSGCGKSTTI
                     RCINRLTEPDEGRIILGGAEITRLKMKELMEVRQRIGFVFQHFNLIRRLSTLDNVAFP
                     LRIQGVSPEEANCRGEAALQKVGLGHKLLETPEELSGGEQQRVGIARALALEPEIMLW
                     DEPTASLDPILVGEVLEVMEELVQEGRTTMLIVTHELFFARKAADRIVFMDKGRVVES
                     GTPEQVLENPVSEIGRKYHHLLHR"
     gene            complement(3133..>3455)
                     /locus_tag="VF531_08000"
     CDS             complement(3133..>3455)
                     /locus_tag="VF531_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713954.1"
                     /translation="YSLFVKFINPATLYALLIGGVWLGFTFALAGAEIPYLNYWIIGL
                     IGSLLGILIATAAERINPVNRDEIMAGLSLGLPFRTIMREIVIPAGRPGLLQLLNRWK
                     MVMK"
BASE COUNT          898 a          975 c          721 g          861 t
ORIGIN      
        1 ttgccaatcc gggtctcgcc gccgccgcct tcggttcccc ggctaatggt aacgttttcc
       61 cggaacatgt tgtcatcgcc aataaccaaa aagctctttt cgcccttgaa tttaagatcc
      121 tgaggttcac agccgataga agcgccatga taaaacttat tgccggaacc aatggtcgtc
      181 cacccgtcga tcataacttg agggccaata acacaattat cgccaatctc aacattctca
      241 ccaatcaccg cataaggacc gatgacgaca ttacgcccga tcttggcact cggatgaaca
      301 atggcggttt catgaatttc acgtaagttt acgacttttg tttcaacgag tttcataata
      361 tataaccccc taaaaattac ccgcctgcca tagggttaaa tccggctctc caacgggata
      421 ataaacgctt aggccaatgt ctgctaatta cggatcgatt tatccattac tcctactaat
      481 taacccggca aaattgcggc atgaggtaaa tacagcacat cacaaaatcc cagattcagg
      541 ttgtttggag taatatgtaa tcggtttatt caggtacaaa taatatcggt cgatccgccc
      601 ccggattgga caggcatatt cggtagattt aggcggttta atccattttc cgcaaattta
      661 cttaatattt cgactcaaac tggcagaatt cgaatttgga aagaaacaat ttttacccgg
      721 ttgatcttcc tttgtatgca catactttaa tcaattattc tcccaagata aacatcattt
      781 ctgcttcggc agctatctgg ttatccactt tggcgaaagc ctgaacccgg cctacattcc
      841 cccggagatg aagcacttct acctcaatga tgatctgatc tccgggtacg attgtccgtc
      901 gaaaacgggc cttgtcaatt ccggtgaaat aaggaacctt tcctttatgt ttttcctcgg
      961 ataacattac cagtcccgcc gcctgagcca tgcattcaac gatcagaacc ccgggcatta
     1021 ccggtttgga gggaaaatgg ccgttgaaaa aattttcatt ggcagtcaca ttcttcagcg
     1081 ccaccactcg cttctccggc tcaatctcaa gaacccggtc cactaggaga aacggatagc
     1141 ggtggggcaa catttttttg atttcgttta tatcaatcat ccggctacct ccttcaattt
     1201 actcattaat acccgactca ggaccaaaac caagtccttg gttaagccgg ggcaagttat
     1261 catgtccaaa ctattttgcc attttacggg ccaactcaaa atcgagagta tggccggaac
     1321 gaacggcgat gatatgcccg cggagcggac cgactaagta taaatccccc aaaatatcga
     1381 gaatcttgtg ccggacgatc tcctcaggga aacgtaactc actttcatag ccatgatcgc
     1441 ccacaaccac caccgagtcg atgtcgccgc ccaaagccaa accctgactt tgtaaatact
     1501 cgatctcccg tcgaaaagcg atcgtccgtg ccggggccac atccgacatg aaattctccg
     1561 gcgtatgttg atactggtag tattgagtcc cggtcaccgg atgatctgag gtaaaagtga
     1621 aactgactcg aaattcatca ctgggtaaag cgatgatcca cgctttcaat ggctgattcc
     1681 cccggggtac cacaccctcg acccagacag gttccttaat acaatagtaa ttacgcggtt
     1741 cactctgttt atgcaatccc actcggagaa actcattggc aaaaaagagt ccgctgcaat
     1801 caccccgggg caactcggct ccgtccacct ctaccaggac attatccact cccaagccgt
     1861 ggaagactgc cattaaatgt tcgatagtgg caatccgcca accatcccgc ccgagtgtca
     1921 tacttttttt cgtgtctacc accgcacttg gctctgcctt gattttggga gatcccggca
     1981 ggtcggagcg gccgaaccat attccggtat tctccggttg cggtgtgatc cgcatcctta
     2041 ccatggctcc agaatgcagg gcctgtccgg taatgtcgaa agcagcagcc acggtttgtt
     2101 gaaattccat tgtcaatccc ccgctgtatc gtggatttcc taccagtaaa cccccgaaaa
     2161 accaaggcgg gctgtttcgt cctggaaccg ctcgccttga tcgaacccgt gctaaatact
     2221 tataaaatta tatgtcagcc cggaagggct gtcaagtacc gccgcagcaa ccaggggctt
     2281 tgatcagctg gaagtttttc cgtttgacta aaaacccact accattcatt ttcaccatga
     2341 aaccaaatta acggtgtaag agatgatgat acttccggcc tatctcagag acaggattct
     2401 ccagaacctg ttcgggggtt cccgactcta caacccggcc tttatccatg aatacgatcc
     2461 gatccgccgc ctttctggcg aagaaaagtt catgggtaac aatcaacata gtggtccgtc
     2521 cttcctggac caattcctcc atgacttcca gcacctcacc gactaaaatc ggatcaagcg
     2581 aagcggtagg ctcatcccaa agcatgatct ccggttccag ggctaatgcc cgggcgatcc
     2641 caacccgctg ttgctcaccg ccgcttaact cttcaggggt ttccaacagc ttatgcccca
     2701 agccgacctt ttgtaaagcc gcctctcccc ggcaattcgc ctcttccggc gataccccct
     2761 gaatccgtaa tggaaaggct acattatcca aagtcgataa acgccggatc agattgaaat
     2821 gttgaaatac aaagccaatc ctctgtctga cctccatcag ctctttcatt ttcaagcggg
     2881 tgatctccgc tccacccagt ataatccttc cctcatccgg ttcagtcagc cggttgatac
     2941 aacggatcgt ggtcgatttc ccgcaaccgc tgggacccat gatgaccacg gtctcgccgc
     3001 ggtcgactgc cagatccact ccgtttaagg cctgttttcg gccgtaactt ttcatcagac
     3061 ccgatatttt taacaatttg aaactcaccc caatccatac tgtgccgcat atctatacag
     3121 accgtgggga tgttatttca tcaccatctt ccaacggttc aacagctgta acagcccggg
     3181 tcgacccgca ggaatcacga tctcccgcat gatagtgcgg aatggcaacc cgagggagag
     3241 accggccatt atctcgtcac ggttgacggg attgatccgt tccgccgctg tagcaataag
     3301 aattcccagt agactgccga taaggccgat tatccaataa ttcagatagg gaatctccgc
     3361 tccggccagg gcaaaggtga atcccagcca aactccgccg atcagaagcg catacaatgt
     3421 cgccggattg atgaacttaa caaataacga ataaa
//
LOCUS       DASVMB010000277         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_400857,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000277 DASVMB010000000
VERSION     DASVMB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400857"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF531_08005"
     CDS             <1..313
                     /locus_tag="VF531_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713955.1"
                     /translation="MDFSNADRETIVDTLNEIKPLITAESSKSVLTLVNVEGLIFNAE
                     ILKAFQDFTKENKPFVKAGAVLGIKGLQKIAYDAVMKVAGRQLPIFDSIDEAKDWLVE
                     Q"
     gene            409..1218
                     /locus_tag="VF531_08010"
     CDS             409..1218
                     /locus_tag="VF531_08010"
                     /inference="COORDINATES: protein motif:HMM:NF022604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX7713956.1"
                     /translation="MHSTNLQPESELKFWLQIMGDHSRFIRDSLLPGQVDLLLVAANF
                     IQIFDRLLERARAVSGGREPGSLVGDATAATLSLRDFKRELLSTRLQNLPVTTLPPTF
                     FNHMLNELEDFLKILAVIEGGRPEQESILGQHLLWSSDAAAHAAIIGSNLDKVESAYR
                     ERSRLFETRFDQLYIKTVEITGYFRSMPPAAQPALESLSEEITIGIVELMGFLEDIGN
                     RVIQKKLLGRISVLQPDHMWREECYYLHQIARATPGMVKPECDPARPRVTG"
     gene            complement(1302..>3521)
                     /locus_tag="VF531_08015"
     CDS             complement(1302..>3521)
                     /locus_tag="VF531_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX7713957.1"
                     /translation="PFYQQLNTLLSTLTFLGLIGTALVYILSKAFWRQGNSWELWPQA
                     KVHLTILAILFLGIKIWGYALAKFGLMYQETARLTGINYAAFHAKLFAYTILTWLLVA
                     IILILLFSFFRRGSKLLIGGLLGWLGASLILLWIYPGAMQSFVVSPNEYELEKPYLEK
                     HIAWTRKAYELDKIDIKAFSPQGDTVQPLNLSNPSLADLRLWDYVPLIPSYNQLQSIR
                     PYYEFHDIDIDRYRTAKGQRQVMIAARELNTAKLSDQAHTWINLHLTYTHGYGMAANP
                     VNQFTSQGQPVFIAHDLPPKVDSAFPSLKVQQPGVYFGELTNDYIIVNTKTSEFDYPQ
                     GDQNISTTYQGTRGIPLASPFIKLLMAFKFQEANFILPSPLTPQSSILIYRNIVERVN
                     KLAPFLIFDDDPYLVVSGGKMLWVIDAYTASAYFPYAKKHSSGLNYIRNSVKAVVDAY
                     TGEVNFYVIDPSDPLIQVWGKVFPRLFKSAAALTPDLVQHFRYPEFLLTIQRDMLLQY
                     HMVDSKTFYEKEDYWDVPAYNQNGQDQLFNPYYVSLKLPGEKAPEFVMMQPFSPRGKQ
                     NLISWLVARCDQPNYGKLQLFVLPKDQNIYGPAQIDSRINQNQTISQLITLWNQQQSN
                     VVWGNILIVPLEDTLLYVKPLFIESETGQQAELKKVVMVYQNQVLLGDTVAEALNQIS
                     SGTISNAASETLPTSAVDQKAAIIRQIESLLKQQLQNQNEQLQITRQLQQLTQDLNK"
BASE COUNT          897 a          750 c          952 g          922 t
ORIGIN      
        1 tatggatttt tcgaatgcgg atcgagaaac aatcgtagac acattaaatg agattaagcc
       61 gttaatcact gccgaatcat caaagtctgt cttaacattg gtcaatgttg aaggattgat
      121 ttttaatgcc gagatactca aggcgtttca ggattttacg aaggagaaca agccgtttgt
      181 taaggccgga gccgttctcg gaatcaaagg gcttcagaaa attgcctatg acgctgtgat
      241 gaaggttgcc ggcaggcaat tacccatttt tgatagtatt gatgaagcga aagactggtt
      301 ggttgaacag taagattttg agaaggtgcc ggttaacttc ccggcatcaa ttaactataa
      361 cacttatcgg gctttacgca tagattacct ccgaaaggag gttttttttt gcattcgacc
      421 aaccttcaac cggagtcgga attgaagttt tggctccaga ttatgggaga tcacagccgc
      481 ttcattcgag attccctact ccccgggcaa gttgatctgc ttttggtagc cgccaatttt
      541 atccaaatat tcgataggtt gttggaacgg gcacgggcgg tttcaggggg tagggaaccc
      601 ggaagtttag tcggggatgc cactgcggcg actttgagtc tacgtgattt caaaagagaa
      661 ttgctctcca cccgtctcca aaatcttccg gtgactacac tccccccgac ctttttcaac
      721 catatgttga atgaactgga ggatttttta aaaatcctgg cagtgataga gggtggaagg
      781 ccggaacagg agagtattct cgggcaacat ttgttatgga gttcggatgc tgccgcccat
      841 gccgcgatca ttgggagtaa cctggataag gtggaatcgg cttatcgcga gcgttcccgg
      901 ctgtttgaaa cacgttttga tcaattgtat atcaaaaccg tggaaataac cggatatttc
      961 cggagtatgc ccccggcggc ccaaccggcg ttagagtcct tgagtgagga aatcaccata
     1021 ggcatcgtcg aactaatggg ttttttggag gatatcggga accgggtcat tcagaagaag
     1081 ctgttgggcc ggatttcagt tttgcagccc gatcatatgt ggcgcgaaga atgctattat
     1141 ctgcatcaga tcgccagggc gactccgggt atggtcaaac cggagtgcga tcccgcccgg
     1201 cccagggtta ccgggtaaat ttaaaagggg ttgcctgaaa gtttttgctt taggacagcc
     1261 ccttctgttt aattattttt gtaatttgaa ttcggaatac tttacttatt cagatcttgg
     1321 gttaactgct gcagttgacg ggtgatctgc agttgttcgt tttggttttg caattgttgc
     1381 ttgagtaaac tctcaatctg ccggatgatg gcggcttttt ggtctacggc agaagttggg
     1441 agtgtctcgg aagcagcgtt tgaaatcgtg ccgctgctga tctgattcaa ggcttcggca
     1501 accgtatccc ctaatagcac ctggttctga tagaccatta caaccttctt caactcggcc
     1561 tgctgaccgg tttcggattc gatgaataac ggcttgacat aaagtaaggt atcttcaagc
     1621 ggcacgatca ggatattgcc ccagacgaca ttggattgct gctggttcca aagggtaatc
     1681 agctgggaga tggtttggtt ctgattgatt ctgctgtcaa tctgcgccgg accatagata
     1741 ttctgatcct tggggaggac aaaaagctgt aactttccgt aattcggctg atcgcaccgg
     1801 gctaccagcc aggaaatgag gttctgttta ccccggggtg agaaaggctg catcatcacg
     1861 aattcaggtg ctttttcccc gggcaatttc aggctgacat aatagggatt aaacagttga
     1921 tcctgaccgt tttgattata ggccgggaca tcccagtaat cttctttttc atagaaagtc
     1981 ttggagtcga ccatatgata ttggaggagc atatcccgct ggattgtcaa gagaaactcg
     2041 gggtagcgga aatgctggac tagatccggt gtgagcgcgg cggcggactt aaagagacgt
     2101 ggaaagactt tgccccatac ctggatcagc ggatcggaag gatcaatcac atagaagttt
     2161 acttccccgg tataggcgtc cacaacggcc ttgaccgaat ttcggatgta gttcaagccg
     2221 ctgctgtgct ttttggcgta aggaaagtaa gcactggcgg tataggcatc aatgacccac
     2281 agcattttgc cgccggagac caccagatac gggtcgtcgt caaagatcaa aaagggtgcc
     2341 agtttgttga cccgttccac gatattgcgg tagatcaaaa tgctgctttg aggtgtaagc
     2401 ggactgggca ggataaagtt ggcctcctgg aacttaaagg ccatcaatag tttgatgaaa
     2461 ggcgaagcga gcggaatacc ccgggttccc tggtaagtgg tagagatgtt ttgatcgcct
     2521 tgggggtaat caaactccga ggttttggta tttacgatga tatagtcgtt ggtcaattca
     2581 ccgaaataga ccccgggctg ttgaaccttt agggacggga aagcggagtc cactttcggt
     2641 ggaagatcat gggcaataaa taccggttga ccttggctag tgaactggtt caccgggttg
     2701 gccgccatac cgtaaccgtg agtgtaggtc agatgcagat taatccaggt atgtgcctgg
     2761 tcggagagtt ttgccgtgtt cagctcccgg gcggcgatca taacctgacg ttgtcccttt
     2821 gcggtacggt aacggtcgat gtcgatatca tgaaactcat aataaggacg aatcgattgc
     2881 agctgattgt aggaagggat cagcgggaca taatcccaaa gtctcaggtc tgccaaggag
     2941 ggattgctga gatttaacgg ttggacggta tcgccctgag gtgaaaatgc ctttatatcg
     3001 attttatcga gctcataagc cttgcgggtc caggcgatat gtttctccag gtatggcttt
     3061 tcaagttcat actcattggg actgacaaca aaagactgca tcgctccggg ataaatccag
     3121 agtaagatca atgaagcccc gagccaacca agcaggccgc cgatgagcag tttgctgccg
     3181 cggcggaaga aactgaataa aaggattaag ataatcgcca ccagcagcca ggtcagaatg
     3241 gtgtaggcga atagcttggc gtggaaggcc gcataattaa tcccggtgag gcgtgcggtt
     3301 tcttgataca tcaacccgaa cttggctaag gcatagcccc agattttaat ccctaaaaac
     3361 aagatggcta agatagtcaa gtgaactttt gcctgcggcc atagctccca ggagttaccc
     3421 tggcgccaga atgccttgga caggatgtaa actagagcgg tgccgatcaa tccaagaaat
     3481 gttaaagtag ataacaaagt attcagttgc tggtagaagg g
//
LOCUS       DASVMB010000278         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_327456,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000278 DASVMB010000000
VERSION     DASVMB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327456"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..556)
                     /locus_tag="VF531_08020"
     CDS             complement(68..556)
                     /locus_tag="VF531_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7713958.1"
                     /translation="MRDKNEVREETRQLVIFKLADEEFGVDILKVNEIEKLDQGITRV
                     PKAPAFVEGVINLRGEVIPIVDLRRRFGLAMREIGYDSRVIIVEAGGNKVGMTVDAVV
                     EVARINESAIEPAPSITKGVNSYYLSGVAKLGERLIVLLDLEKALTAEEASEIGMMKT
                     EA"
     gene            complement(584..2527)
                     /locus_tag="VF531_08025"
     CDS             complement(584..2527)
                     /locus_tag="VF531_08025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX7713959.1"
                     /translation="MEVSQYLSVFMDECQEHLQTLNQAILELEHNPENSGVLDQIFRA
                     AHTLKGASATMGFNKMANLTHAMEDVLSKLRSKELTASTEIVNVLFEAIDMLEVLASK
                     IGEGIEEDIEITGVVQELLRFVNGETVPQAPDRRHNLQLRYLEDEKKQISSGLENGLK
                     FFQITVTLVENCLLKGARVFMILRDTEKLGTIIRSVPTVKELEDENFDLQFLVGILSN
                     KTPEEIIKTISNISDVDKVQVDEPKFDQLLTEKRVQEVVDQGEKKVTSIQTVRVDIKK
                     LDDLMNLVGELVISRSQLESLGTAIGSKDLDEVVEQIGRLTMDLRDSVLKARMVPVET
                     VFSRFPRLIRDLCKELGKEINLEIHGGETELDRTLIDEIGDPLVHIIRNSADHGIETG
                     EERARNNKPANGKLVLNAFQEGNSVVITVSDDGRGFDIKKIKEKAIARGLVTPEVLSE
                     MSPGQILEFTFLPGFSTAEKITDVSGRGVGMDVVKTKINSLKGVVSIESEPGQGSTIT
                     IRLPLTLAIIQTLMIQLGKETYAIPASYVEQLVNVKRSDVHFIHKQEVLSLRGEIVPL
                     LRLQDILDTPGALNPQMDELDVVILKVGERMVGCIIDSFLGQKDVVIKSLGGFLGSIK
                     GIAGATILGDGKVALILDIREVA"
     gene            complement(2545..>3225)
                     /locus_tag="VF531_08030"
     CDS             complement(2545..>3225)
                     /locus_tag="VF531_08030"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HEX7713960.1"
                     /translation="TRKTSRNGVVKMEFNVGDYVEIEIPDGYFHGEYSSRIIFLDQQQ
                     LVITTPRMGGAIVSLRQGQEIHLSVPKRDARYNFEAVVIDVNESRPDLLTLKLDTTAI
                     RQQRRSDVRLTVRLPVELLYFYRQGVPVASYTVYSIDVSAGGIKLEMSEEYPPHTHFK
                     LAIHLPEDEIAIQATVIRSGILPTTDAGGNPSFWVSLKFFNISEAKQKKILKFIYKQQ
                     ELRVKGLI"
BASE COUNT          844 a          845 c          649 g          887 t
ORIGIN      
        1 gccgatattt ccaacttcct tcaagatatc caattggaac cgactgaggt cttcaaatct
       61 taacatttta agcctccgtc ttcatcatac caatctccga agcctcctcg gcggttaatg
      121 ccttttccaa atcgagcagg acgattagtc tttctcccaa tttcgcgact ccactcaaat
      181 aataagaatt gacacctttg gtaatggacg gagcaggttc aatcgcggat tcgttaatcc
      241 gggcgacttc aaccacggca tcaaccgtca ttccgacttt attgccaccc gcttccacga
      301 tgataacccg tgaatcataa ccgatctccc gcatagccaa accaaaacgg cggcgaagat
      361 ccacaatggg gattacctca ccccgcaaat tgataactcc ttcaacaaag gcgggagcct
      421 ttggtacccg ggtgattcct tgatccaact tctcaatctc gttgactttg agaatgtcca
      481 ctccaaattc ttcatcagcc agtttaaata tgacaagctg gcgggtttct tctcgaactt
      541 cgtttttatc tctcatcgta ctccccccaa tctttatgct acctcatgct acctcgcgga
      601 tgtctaaaat caaggcaact ttgccgtcgc ctaaaatcgt agcaccggcg atccctttaa
      661 tcgaacccag gaaaccgcca agtgatttaa taaccacatc tttttggccc aagaaactat
      721 cgataatgca gcctaccata cgttccccga cctttaagat aacgacatcc aactcatcca
      781 tctggggatt aagcgctcca ggggtatcga gaatatcctg aagacgcagt aacggtacga
      841 tttcaccacg aagcgacaaa acctcttgtt tatgaatgaa atggacgtca ctccgtttta
      901 cattgactaa ttgttccaca taactggctg gtatggcata agtctctttc cccaattgaa
      961 tcatgagagt ctggatgatg gcgagtgtca aaggcaaccg gatagtgatg gtcgagccct
     1021 gtcccggttc ggattcgata gaaacaactc cttttagcga gttgatcttg gtcttaacca
     1081 catccatacc tacgccgcgt cccgagacat ccgttatttt ctcagccgtg gaaaagccgg
     1141 gtaagaacgt aaactctaga atctgaccgg gactcatctc cgataaaact tccggggtaa
     1201 ccaagccccg ggcgatagcc ttttctttga tttttttgat atcaaatcca cggccatcat
     1261 ccgaaacggt aatgactacg ctgttgcctt cctgaaatgc attcaaaacc aatttgccgt
     1321 tggccggttt attattcctt gcgcgttctt ccccggtttc gattccatgg tcggcgctgt
     1381 ttcgaatgat atgtaccaat gggtcgccaa tctcatcaat taaggttcga tcgagctccg
     1441 tctcgccgcc gtggatctcc agattaatct ctttgcccaa ctctttacag agatcacgga
     1501 taagacgggg aaaacgtgaa aagaccgttt ctaccggaac catgcgggct ttcaagacgc
     1561 tatcccgtaa gtccatcgtc aaacgtccga tttgctcgac aacttcatca agatctttgg
     1621 agccgatagc cgtaccaagt gattcaagtt gagaacggct gatcaccaat tcaccgacga
     1681 ggttcatcag atcatccagt ttcttaatgt ccacccggac cgtttgaata ctggttactt
     1741 tcttttcccc ttgatccact acttcctgga cacgtttttc agtcagtaac tgatcaaact
     1801 tcggttcatc cacttgaacc ttgtccacat cagagatgtt tgaaatggtt ttaatgatct
     1861 cttccggggt tttatttgat agaatgccga cgagaaactg gagatcaaaa ttttcatcct
     1921 cgagttcctt gactgtaggg acgctccgga tgatggtgcc tagtttttcg gtatcacgca
     1981 aaatcataaa tacccgggcg cctttgagga gacaattttc cactaaagtg acagtgatct
     2041 gaaaaaactt caacccgttc tcaaggccgg aggagatctg ctttttctca tcctccaaat
     2101 accgcagctg taaattatga cggcgatccg gagcttgggg aaccgtttct ccgttaacaa
     2161 aacggagtaa ttcttgaact acgcctgtta tctcaatgtc ttcttcaatg ccctcgccga
     2221 tcttggaagc caatacctcc aacatgtcga tggcttcaaa aagtacattg acaatctcgg
     2281 tggaggcggt aagttccttt gaccggagct tgcttaaaac atcctccata gcatgggtca
     2341 aattagccat cttgttaaag cccattgtgg ccgatgcgcc tttcaacgta tgcgcggcgc
     2401 ggaaaatttg atccaacacc ccggaattct ccggattatg ctcaagttcc aatatcgcct
     2461 ggtttagagt ctggaggtgt tcttgacact catccataaa cacactcaaa tattgggaaa
     2521 cttccataat cccaccccca aaggttaaat tagacctttt acccttaatt cctgctgctt
     2581 gtaaataaat ttaagaatct tcttctgttt cgcctcagag atattaaaaa atttcagact
     2641 cacccaaaaa cttggattcc ctccggcatc cgtagttggt aaaataccac tacgaatcac
     2701 cgttgcctgg attgcgatct catcttcggg aagatggatt gccagtttga aatgggtatg
     2761 aggcggatat tcctccgaca tttccagttt gattccgccg gcgctcacat cgatgctgta
     2821 tacggtatat gaagccaccg gtacaccctg cctataaaaa tagagcaatt ccacaggaag
     2881 gcggactgtt aaacgaacat ccgaccggcg ttgttggcga atggccgtcg tatccagttt
     2941 tagagtcagc aaatcgggtc ttgattcgtt aacatcaatg acaacggctt caaaattata
     3001 ccgggcatcc cgtttcggaa cagagaggtg aatctcttga ccttgtctta atgagacgat
     3061 ggcgccgccc attcgcggtg ttgtaatcac aagttgctgt tgatccagga agatgatccg
     3121 actggagtac tctccatgaa aataaccatc cgggatctca atctccacat aatcgccaac
     3181 attaaactcc atcttcacta ctccgttccg tgaagttttc cgagt
//
LOCUS       DASVMB010000279         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_549443,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000279 DASVMB010000000
VERSION     DASVMB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549443"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VF531_08035"
     CDS             <1..1474
                     /locus_tag="VF531_08035"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX7713961.1"
                     /translation="GLSKKLIYLELTGRNANLILTTENDRIIDSWRRVSSARPGGREL
                     LSDSRYLLPPSGGRWQPVSIGVEQFSALLNQVPADVPLEKFLWKHWNGLSALAIGEIT
                     AAAGFSPLTTCAQLNPTGITALYEAFSSWAAPLNAGIYEPACLYPEGSSEPTDCAAFA
                     VHYPPQGIVARPVPNLLEVVAVIGNSRHEKHRFLETREHLNHRVDLLLEKARLKWEKQ
                     QDEAAAADKGDALRIAGELLITSAHQISKGTTEARLTNYYDPAGGTLTVALNPALTAA
                     QNAQVYFKKYQKAKKGQLAIAEQLAKTQAVIEYLESIVTLIQNAANFSDLELVREELD
                     QNQSESRLRPTGKGKAKPVSKKTQPAEPRRFTTSSGHIIWVGRNNLQNDRLTFKMAAP
                     GDLWFHTQKIPGSHVILKLNPGTPLDDTTLNSVCQLAVYFSKARNSVKVAVDYTQRKH
                     VKKPPGSKPGFVIYDDFKTAIITPDPVILSQLGVTSSASQ"
     gene            1628..2092
                     /locus_tag="VF531_08040"
     CDS             1628..2092
                     /locus_tag="VF531_08040"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX7713962.1"
                     /translation="MLPTPRKYDLVTGFGEGNTTLTAFDAALLNSGLGNLNLLKVSSI
                     LPPHAEFTPGLIIPPGSLVPTAYGAICSQQPGERIAAAVAVGIPKEADSYGVIMECSG
                     CCAREELEQRIRKMVIEAFQKRDLALKEIQVSAVDHRVNECGCAFAGLPLWY"
     gene            complement(2206..2637)
                     /locus_tag="VF531_08045"
     CDS             complement(2206..2637)
                     /locus_tag="VF531_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7713963.1"
                     /translation="MNAFLLALFGALCWGLAPLFGKVGLRGLNPLDGIVARTMITVLF
                     IIGLAFASGFIHRVGTIPAKRWFLLAAEAFLATFAGDIAYYGAIKKGEIGQTAIIMAA
                     APLFTLWVGWYFLGEQLSPMKLVGAGLIIIGLILIGFKSWN"
     gene            complement(2654..3661)
                     /locus_tag="VF531_08050"
     CDS             complement(2654..3661)
                     /locus_tag="VF531_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX7713964.1"
                     /translation="MDDKRYRWLLGAVFVFLVGAGFGVSRFWLSHQPQKNDKAKPRGP
                     ISRSIPLKSSGFPDPSPSDNTRSKPVPDGTVFDGADHPYGRPAKGVLRWENNRAFHEF
                     CSKHHISLRMAAFQTTLPDPLPGEEFNVALAADLLAGTVVEPGRVFSMNGTIGPYTKD
                     RGFQEGPAYFGTQVFKTIGGGVCKIATTLYNVTTLANLEIIERRPHGMPVPYVPPGQD
                     ATVSDGAKDFKFRNNTGQPLFIWADTHENTLFMAIYGRTKPPKVEWRHQILRRQKNRI
                     IHRVNRELPPGTQRVVIPGADGLTVKSWLIITYPEGRVNRRDLGIDTYQPLPEVRESG
                     R"
BASE COUNT         1028 a         1033 c          965 g          928 t
ORIGIN      
        1 agggctgagc aaaaagctga tttatctcga attgaccggc cggaatgcca acctcatcct
       61 taccactgaa aatgaccgga tcatcgactc ctggcgtaga gttagttctg ccagacccgg
      121 aggccgcgaa cttcttagcg attcccgata tcttttacct ccgtcgggag gccgttggca
      181 accggtgtct atcggtgttg aacagttcag cgccttattg aatcaggtac cggccgatgt
      241 gcccttggag aaattccttt ggaaacactg gaacgggctc tccgctttag cgataggtga
      301 gatcacggct gcagccggct tttcaccgct aactacctgc gcccagttaa acccgaccgg
      361 gatcacagcc ctctacgaag ccttttcttc ctgggcggct ccactgaatg ccggcatcta
      421 tgaaccggcc tgcctatatc ctgagggttc gagcgaacca actgactgcg ccgcctttgc
      481 ggttcattat cctccacaag ggatcgttgc ccgcccagtg cctaatcttt tggaagttgt
      541 ggccgtgatc ggcaactccc gtcatgaaaa acatcgcttt ttggaaaccc gggaacatct
      601 aaatcaccgg gtcgatctgt tattggagaa agccaggctg aaatgggaga aacaacagga
      661 tgaagcagca gcagccgata aaggggacgc gttacggatt gccggtgaac ttttaattac
      721 ctccgctcat cagatttcca aaggtacaac cgaagcccgt ttaaccaatt attacgatcc
      781 ggccggaggg acgctcactg tcgcccttaa cccggctttg acggcggcac aaaacgccca
      841 agtatatttc aaaaaatatc agaaagccaa aaaaggacaa ctggcaatcg cggaacaact
      901 cgccaagact caagcggtca ttgaatattt ggagagcatt gttacattaa ttcaaaatgc
      961 ggccaatttc tccgatcttg aactcgtccg ggaggaattg gatcaaaacc agtccgagtc
     1021 aaggctgcga ccgacaggaa agggtaaggc caaacccgtc tcaaaaaaga cgcaaccagc
     1081 cgagcccaga cggtttacca cctcaagcgg acatatcatc tgggtcggac ggaataacct
     1141 tcaaaacgac cgcctgacct ttaagatggc cgcccccggt gacttatggt ttcataccca
     1201 gaagatcccc ggatctcatg ttattttaaa actaaacccc gggactcctc tagatgatac
     1261 aaccctgaac tccgtttgcc aacttgcggt ttattttagt aaagcccgta attccgtcaa
     1321 ggttgcggta gattataccc aacgcaagca cgtaaaaaaa ccccccggat ccaaaccggg
     1381 ttttgtgatc tatgatgatt tcaaaaccgc gatcatcacc ccggatccgg taatcctttc
     1441 ccaactggga gtgacatcct ctgccagcca ataaatcgaa tgtctcaaga attgcgctat
     1501 taggccccag gcgtacgtat cacgcacatc tgccctcgct ttttcaaaag cgagggcaga
     1561 tgtgtaccga tttcgagaca ggcgcatatt ctataaaggc tagcactttt taaggaggta
     1621 tttgcgcttg ttgccaacac cgcgaaagta cgatttagtg acgggattcg gagaaggaaa
     1681 caccacgttg accgcctttg atgcggcatt gctcaactcg ggattgggca atctcaattt
     1741 actcaaggta tccagtatcc tgccacccca cgccgaattc accccgggac ttattattcc
     1801 gcctggttcg ctggttccaa ccgcatacgg cgcgatatgc agccaacaac cgggagaacg
     1861 aattgcggcc gccgtggctg tgggtattcc caaagaagcc gattcttatg gggtgatcat
     1921 ggagtgttcc ggttgttgtg cccgggaaga actggaacaa cggattcgga agatggtaat
     1981 tgaggctttt caaaaacgcg acctggcatt aaaagagatt caagtgagcg ccgttgatca
     2041 ccgggtgaat gagtgcggct gcgccttcgc cggactcccc ctgtggtatt gagctaaaaa
     2101 gagtgaagtg tgaagtcgga agtatgaaat aatccggagt tcaagttttt gcctcaagaa
     2161 cagccgtaaa atccggagaa tctgttaaaa tccacctaat cccggtcagt tccatgactt
     2221 gaagccgatc aagatcaatc cgatgatgat caacccggct cctaccagtt tcatgggcga
     2281 gagctgttct cctaaaaaat accatccaac ccatagggtg aatagtggag ctgcggccat
     2341 aattattgcg gtttgtccga tctctccttt tttaatggct ccgtaataag cgatgtctcc
     2401 ggcaaaggtc gctaaaaacg cctcagcggc taaaagaaac caccgtttgg cgggaatggt
     2461 ccctacccga tgaatgaatc cgcttgcgaa ggccaaaccg ataatgaaca atacagtaat
     2521 catagtccgg gcgacaattc catccaaggg attcaagcct ctaagaccaa cttttccaaa
     2581 taaaggagcg agtccccaac ataaagcacc aaacaatgcc aggagaaaag cgttcaatcc
     2641 tcatcactcc cgattatctg ccgctttccc gtacctccgg taaaggctga taagtatcga
     2701 tccccagatc acgccggttc acccgaccct caggataagt gatgatgagc caggatttca
     2761 cggttaaacc atcagcaccg ggaatcacaa ctctttgggt acctggtggt agttctcgat
     2821 tcacacgatg aatgatccgg tttttttggc ggcgtaaaat ttgatggcgc cattcgacct
     2881 tgggaggttt ggttcgaccg tagatggcca taaataaagt attttcatga gtatcagccc
     2941 aaatgaacaa cggttggccg gtgttgtttc taaatttaaa atctttggct ccgtcggaga
     3001 cggttgcgtc ctgaccggga ggtacatagg gtaccggcat gccgtgaggt ctacgttcga
     3061 tgatctccag gtttgcgagg gttgttacat tatataaggt ggtagcaatc ttacagaccc
     3121 caccgccgat ggtcttaaag acctgagtgc cgaaataggc cgggccttcc tgaaaacccc
     3181 ggtcctttgt atatgggccg atcgtgccgt tcattgaaaa aacccggccg ggctcgacaa
     3241 ccgttccggc cagcaaatca gctgccagtg ctacattaaa ttcttcaccg ggcaacgggt
     3301 cgggtaaagt ggtctgaaag gccgccatcc gcagtgatat atgatgtttt gaacagaact
     3361 cgtgaaaggc gcggttattc tcccatcgta agaccccttt ggccggacgg ccgtacggat
     3421 gatcggcgcc atcaaacacg gtaccgtccg gtaccggttt ggagcgggta ttgtccgagg
     3481 gtgacgggtc cgggaacccg gagcttttaa gcggaatgga ccgcgatatc ggtccgcgag
     3541 gttttgcctt gtcatttttt tgcggttggt gggagagcca aaaacgagat actccaaaac
     3601 cagcccccac caagaaaaca aaaacggccc ccaacagcca tcgatatctc ttatcatcca
     3661 cccaaacagc ctccgtgttc ttcactcgca cgcgtgatca atctgatcca tagttattcc
     3721 ccaaaatcca aatcatgagt tgatctttcc ggggctttgg atcgattggt cggacagcta
     3781 acaaatcact gctgcggacc atataataca aaagtgtcat gccaaaaggc gcgcaaattg
     3841 gcacgtgcag aagcgttgcc cgtacgttta aaccttctcg atcggagttg attcgatggt
     3901 cttttccatt atgctgttgc ccgtatcatt aagcctggat gctttgggag tagg
//
LOCUS       DASVMB010000280         6183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_401054,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000280 DASVMB010000000
VERSION     DASVMB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401054"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VF531_08055"
     CDS             <1..932
                     /locus_tag="VF531_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7713965.1"
                     /translation="VLKGDPAAVRVPKEVDIRIGDVTDAESVERFFKVDDGTAIVVIH
                     CAAIVTVNPDFSQKVYDVNVGGTKNILNACIAHKVKKLVYVSSISAIPELPHGTAITE
                     VDRYDPNVVVGFYGKTKAEASQAVMDAVNGHGLDASIVFPTGICGPDDYAFGPVASFI
                     IDYCAGKMPGGVAGSFNAVDARDLADAIVTCCEKGRKGQGYILGNECVTMQQMFHLLS
                     TLTGTKEVKMILPVGAARILGKLSDLAGKITKKPMRMTSFAVYNLIRNNVFDCSKAER
                     ELSYHTRLFEDSIADTIAWLTREHKIQTGRKAS"
     gene            929..1756
                     /locus_tag="VF531_08060"
     CDS             929..1756
                     /locus_tag="VF531_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7713966.1"
                     /translation="MKKVCVITGGGSGIGLATAKNIGNQGYYLILVGRTAKKLENAVT
                     ELQAMGIEAEMFNCDIADRQSTRLLAATALERGEVKVVIHAAGMSPQMGDAQKIMEAN
                     ALGTININDAFYEVMHDGGCIIDTSSMSAYLTPQFIMPKHCYKLSRTDRDEFMKKMMR
                     RVNIFPKKCRAGVAYGISKNFVIWFAKTDAARFGQKGIRVLSITPGNFETPMGELERD
                     EAMTYVKYNAIKRLGHPEEIAQLYVAAADDRMGYLTGTDILCDGGCIASGCTPFKRS"
     gene            1810..2343
                     /locus_tag="VF531_08065"
     CDS             1810..2343
                     /locus_tag="VF531_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713967.1"
                     /translation="MNEITKKENNFVGYEYKDVTVKHHMEPVYADGYIDFGWSLEGTS
                     TPIQNVGSVIMKFKRDRKIRNKAELSRLQRQFDSCAREIETLELSKVIAASAVAYIIG
                     VAGTAFIAGSVFAYLASKLPLSIILAIPGFVGWIIPYFCYTSIRNKKNDEVTPLIDKK
                     YDEIYEVCEKANNLLEK"
     gene            2391..2825
                     /locus_tag="VF531_08070"
     CDS             2391..2825
                     /locus_tag="VF531_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713968.1"
                     /translation="MKTQKISLLVLLTVTLMTLVCSTALADSTITTDFFGDVHGQYNV
                     TFETNLGILPDNYTFYGTYYAYSGSFPNRAVIGIDSFLGEPFNGLYAGVGIGIPSGNR
                     SSSTGMDIKVGYKKIFKNGFALDGGYSYYYNNNYYLQVGLAF"
     gene            3000..3806
                     /locus_tag="VF531_08075"
     CDS             3000..3806
                     /locus_tag="VF531_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7713969.1"
                     /translation="MNEKRYLVGEIATITGLTVRTLQHYDNIGLLPVSGRTEGGRRFY
                     TESDLVRLEQIIFYKMLDFTLEEIKETLMDSSSLSELEDILKNQEFLLLRKIEQLHTS
                     YTTIDVCMKMINAGKKPPFHVLLKFIKTLPGDDVFEWGPSMLSQEQQSIMSESFKDPE
                     DVQRFLHKLKAMLIGATALLNSGFSPAHEQAQILAEQWMDMILSISDGNSKSLEQFIQ
                     IGKASESKQKGEKQLMDDAYSYIQQVCAIYTANHNLPNINQMKCGTQNKT"
     gene            3870..4913
                     /locus_tag="VF531_08080"
     CDS             3870..4913
                     /locus_tag="VF531_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7713970.1"
                     /translation="MKKIHQTLNHTNESKGLTAGILKHKWCAGIVGFIIMIFLTIGGS
                     IIVAHDPSPVGHFRSRQGERAYKQAYANAMKLLPAPSRTLDVRTDYGTVRVYEFANSR
                     NQAGAPIILLPGRSSGVPMWASNLKDLAAERSVFALDALGDAGLSVQTRKLENSSDQA
                     AWLDQVFRQVGLSQVHLVGHSFGGWLAANYAARYPERVVTLDLLEPVFVLQGLRWQIY
                     LKSIPASIPFLPESFRKRMLGDIGGVAEIDLNDPIARMIADATKYYAAKLPAPDRISE
                     EQLKGLSMPVYAALAANSSLHDPIAAVRVAKANIRTIQVRNWPGATHSLPMEFPRQVD
                     RELLNFIAANETR"
     gene            5028..>6183
                     /locus_tag="VF531_08085"
     CDS             5028..>6183
                     /locus_tag="VF531_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7713971.1"
                     /translation="MVLTLLLIIIYLSFISLGLPDSLLGSAWPSMYLRMGVPVSYAGI
                     VSMIIAGGTIVASFFSERIIRRLGTGKITAISVSMTAIALLGFSVSRNFFELCIWAVP
                     LGLGAGSVDAALNNYVALHYKAKHMSWLHCFWGIGATTGPVIMSYCLQNGGRWNRGYL
                     TIGIIQFCLVAVLFLSLPLWKKVGEKSAREGEIQKSSLKLSELLKLPGAKPALVSFFC
                     YCSLEATAGLWGSSYLVIAKGITAETAAKWISLFYFGITFGRFLSGFITMKLNQRQMI
                     RLGQAVLACGVILLFLPFGNFVLLSGFFLIGLGCAPIYPSLLHETPVNFGSKHSQAIM
                     GIQMACAYAGSTFMPPLFGVLAAHFNYAMFPLYLGVILLVLIAMVEVLNKR"
BASE COUNT         1644 a         1337 c         1531 g         1671 t
ORIGIN      
        1 tggtgctgaa aggcgaccct gccgcggttc gtgtgccgaa agaagtggac atccgcatcg
       61 gcgacgtgac ggatgcggaa agtgttgagc gtttcttcaa ggtggatgac ggcaccgcaa
      121 tcgtggtgat ccactgtgcg gctatcgtga ccgtcaatcc cgacttcagc cagaaggtat
      181 acgatgtgaa cgtcggaggc acaaaaaaca ttctgaacgc ctgtatcgcg cacaaggtga
      241 aaaagctggt gtacgtcagc tcgatcagtg cgattccgga attaccgcat ggcactgcta
      301 ttactgaggt ggatcgctat gatcccaatg ttgtcgtcgg attttacggc aaaacgaaag
      361 ccgaggcatc tcaggctgtg atggatgccg taaacgggca tggcttggat gcgtccatcg
      421 tgtttcctac cggtatctgc ggccctgatg actacgcctt tgggccggta gccagtttca
      481 tcatcgacta ctgtgccggc aaaatgcccg gcggtgtggc aggcagtttc aacgccgtgg
      541 atgcgcggga tctggctgac gccatcgtga cctgctgtga gaaaggccgc aagggccaag
      601 gctatattct aggtaatgag tgcgttacca tgcagcagat gtttcatctg ctcagcacgc
      661 tcacaggtac aaaagaggtc aagatgattt tacctgtggg tgctgccagg atcctcggaa
      721 agctatctga tttagccggg aaaatcacca aaaagcccat gcgcatgacc agttttgccg
      781 tatacaacct gatacgtaac aacgtattcg attgctcaaa ggcagagcgc gaactgagtt
      841 atcatacgcg tctctttgag gactccatcg cggataccat cgcatggctt acgcgcgaac
      901 acaaaattca aaccgggagg aaagcatcat gaaaaaggtt tgcgtcatca ccgggggcgg
      961 cagtggtatt ggcttggcaa cagctaaaaa tataggcaac cagggttact atctcatcct
     1021 ggtaggccgt acggctaaga aactggaaaa cgccgtaaca gaattacagg ccatgggcat
     1081 tgaagccgag atgttcaact gtgacatagc cgatcgtcaa agcacgcggt tgttggccgc
     1141 aaccgcactt gaacgcggcg aggtgaaagt agtgattcat gcagcgggga tgtctcctca
     1201 aatgggcgat gcacagaaga tcatggaagc aaacgcactt ggcactatca atatcaatga
     1261 cgcattttat gaagtgatgc atgacggtgg ctgtatcatc gatacttcat ccatgtccgc
     1321 ctatcttacg ccgcaattta tcatgccgaa gcattgttat aaacttagcc gtactgatcg
     1381 ggacgaattc atgaagaaaa tgatgcgccg cgtcaatatt ttccctaaga aatgtcgtgc
     1441 cggtgtggca tacggcatca gcaaaaactt tgtgatctgg tttgccaaaa ccgacgccgc
     1501 ccgcttcggc caaaagggta tacgcgttct ctccatcaca cccggcaact ttgaaacgcc
     1561 tatgggcgag ctggaaagag atgaagccat gacttatgta aaatataacg ctatcaagcg
     1621 cttgggacac ccggaagaaa ttgcgcagct atatgttgcc gcagccgatg atcgcatggg
     1681 ttacctgacc ggcaccgaca tcctgtgcga cggtggttgt attgccagcg gttgcacccc
     1741 gtttaagcgt tcttaactta atcagaaaag atacgttaaa taaactctaa aaatgtagga
     1801 ggaatcaata tgaatgagat cacgaaaaaa gaaaataatt ttgtgggtta tgagtacaag
     1861 gacgttacgg taaaacatca tatggagccg gtgtatgcag acggctatat cgattttggc
     1921 tggtcgctgg aaggcacctc cactcccata cagaacgttg gttccgtaat catgaagttt
     1981 aagcgcgacc gcaagattcg caataaggcg gagttgagca ggttacagcg tcagttcgat
     2041 tcatgcgcca gagaaattga aacgttggag ctttccaagg tcatcgctgc ttctgccgtt
     2101 gcgtacatca ttggcgttgc aggtacggca tttatagccg gttccgtgtt tgcttatctt
     2161 gccagcaagc tgcccctttc catcattctg gcaatccctg gttttgtagg atggatcatc
     2221 ccgtatttct gctacacgag tatccggaac aagaaaaatg atgaggtaac gccgttgatc
     2281 gacaagaagt atgacgaaat ctacgaggtg tgcgaaaagg ccaataactt gctggaaaag
     2341 taacatccga aagaacaatt tacttatatc tttatattaa ggagatttcc atgaagacac
     2401 agaaaatttc tttattggtt ctcttgactg taacattgat gactttagtc tgtagcacgg
     2461 ctttggctga ctccacgatt accacagatt tctttggtga tgttcacggc cagtataatg
     2521 tcacgtttga aaccaacctt gggattttac cggataacta cactttttac ggtacttact
     2581 acgcatatag cggcagcttt cccaataggg cggttatcgg catagactca tttttgggag
     2641 aaccttttaa tggtctctat gcgggtgtag gaattggtat accaagtgga aatcgttcat
     2701 cttctacggg aatggatata aaagttggat ataaaaagat cttcaaaaac ggatttgcgc
     2761 ttgacggcgg ttatagctac tattacaaca acaattacta tctccaggtt gggctggctt
     2821 tttgactcac tggctaaacc tcagtcggat ctatttctta ttcacaacga gacatataaa
     2881 agatatgaaa gtaggaaaag accatcaaac tatagcgttt ggtggctttt atttttttta
     2941 tttcaccgct tgcatattac gtaacgtaat gtgatattct gattttagag gtgagcggtt
     3001 tgaatgaaaa acgttattta gttggagaaa ttgctacgat taccgggctg actgtgcgga
     3061 cattgcagca ttatgacaat atcggcctct tgcctgtatc gggaaggacg gagggcggca
     3121 ggcgttttta tacagagtcc gacctggttc gcttggaaca aatcattttt tacaagatgc
     3181 tggatttcac cttagaagaa atcaaagaaa cactgatgga ctcctctagc ctgtctgaat
     3241 tggaggatat attaaagaat caggagtttt tgcttttacg aaagattgaa cagttacata
     3301 cctcatatac aaccatcgat gtctgcatga aaatgatcaa tgcagggaaa aaacctccgt
     3361 tccatgtctt gctaaagttt atcaaaacat taccgggtga tgatgttttt gagtggggac
     3421 catccatgct ttcgcaagag cagcagtcaa tcatgtcgga gtcttttaaa gatccggagg
     3481 atgtacagag atttcttcat aaattgaaag ccatgttaat tggggcaaca gctctgctca
     3541 attcgggttt ttctcccgcc catgaacagg cacagatctt ggcagagcaa tggatggata
     3601 tgatcttatc catctccgac ggcaattcta aatccctgga gcagttcatt cagatcggaa
     3661 aagcaagtga atctaagcag aagggtgaaa aacagctgat ggatgatgca tatagttata
     3721 tacagcaggt atgtgctata tatacggcaa accataatct gccaaatatc aatcaaatga
     3781 agtgcggcac acaaaataag acttaatatc attatctaat tatatttcaa gaaatttagt
     3841 tcaacatata aaaaagagag gtcggtatca tgaaaaaaat acatcagaca ctcaatcata
     3901 ccaatgagtc aaagggatta accgccggta tactcaagca taagtggtgt gccggtatcg
     3961 tgggatttat cataatgatc tttttaacta tcggtgggtc gataattgtg gcacatgatc
     4021 ccagtccggt tggccatttc cggagccggc aaggcgaacg ggcctataaa caagcctatg
     4081 caaatgccat gaagctactc cctgctccca gtcgcacact ggacgtcaga accgactacg
     4141 ggacagtacg ggtttatgaa tttgccaatt cccggaacca agcgggggct ccaatcatat
     4201 tgcttcccgg acgttcgtca ggtgttccta tgtgggcatc caatttaaag gatcttgctg
     4261 ccgagcggag tgtttttgca ctggacgcat taggtgacgc aggtttgagt gtacagactc
     4321 gtaaacttga gaattcctcc gaccaggctg catggcttga tcaagtgttt cgccaggtgg
     4381 gattgtcaca agtccattta gtcggccatt catttggagg atggctagcc gccaactatg
     4441 cggcacggta cccggaacgt gttgttacgc tcgatctcct tgagccggtt tttgttcttc
     4501 agggactgcg ctggcaaatc tatctcaagt cgattcctgc ctcaatacct tttttgcctg
     4561 aaagctttcg taaaagaatg cttggggata ttggcggggt ggcggagatt gacttgaatg
     4621 atccgattgc ccggatgatt gcggatgcaa ccaaatatta tgcagctaag ctacctgcac
     4681 cggatcgcat atcggaggag cagctaaaag ggttaagtat gccggtatat gcagccttgg
     4741 ctgctaatag ttctctccat gacccaatcg cagcagtcag ggtagccaaa gcgaatatta
     4801 ggaccattca agtgaggaat tggcccggcg ctacccattc attgccgatg gaattcccta
     4861 ggcaggtaga ccgtgaattg ctcaatttta ttgcagccaa tgagacgaga taatcaagat
     4921 tagtcatttg cacctgtctt tcctgtcttt agcaaacttt cctccaggca ggaaaagcag
     4981 gatagccggc gaatgactat tcagtcttca ccgtaaagga gcatatcatg gtcttaacat
     5041 tattgctgat tattatttat ctttccttca tcagtcttgg cttgcccgat tccctgctgg
     5101 gttccgcatg gccatccatg tatctgagaa tgggcgtacc ggtttcctat gccggtatcg
     5161 tctcgatgat tattgccggg ggtacgattg ttgccagctt ttttagtgag agaatcataa
     5221 ggcgtttggg aaccggtaag ataacggcaa tcagtgtatc gatgacggca attgctttgc
     5281 ttgggttttc cgtaagccgt aatttttttg aactatgcat ctgggcagta ccgttaggac
     5341 ttggagccgg ttccgtcgat gcggctctga ataattatgt cgcgcttcat tataaagcga
     5401 aacacatgag ctggctgcac tgtttttggg gaatcggcgc caccacggga ccggtcatta
     5461 tgtcatattg tttgcagaac ggcgggagat ggaaccgggg ctatctgacc atcggcatta
     5521 ttcagttctg ccttgtggca gtactgtttc tttccctgcc gttatggaaa aaagttggtg
     5581 aaaaatcggc acgggaaggg gagattcaga agtcatcctt aaagctgagt gaattattaa
     5641 agcttcctgg tgccaaaccg gcactggtat cttttttctg ttattgttcc ctggaggcca
     5701 ctgcaggatt atggggcagc agctatctgg ttattgcaaa gggaattact gctgaaacgg
     5761 ctgcaaaatg gatttcactg ttttactttg gaattacttt cggaagattt ctttccggtt
     5821 ttattacaat gaagctgaat caacggcaga tgatacgttt gggacaggca gttttagctt
     5881 gtggagttat acttttattc ctcccgttcg gaaacttcgt actgctctcg ggatttttct
     5941 taatcggact tggttgcgca ccaatttatc cgagcctgct tcatgaaaca ccggtgaatt
     6001 tcggcagtaa acactcccag gcaatcatgg gcatacaaat ggcatgtgcc tatgccggtt
     6061 cgacttttat gccgccactc tttggtgtgc ttgcagcgca tttcaattac gccatgtttc
     6121 cgctatatct cggggtaata ttactggtat tgatagcgat ggtagaagtt ttaaacaaaa
     6181 ggg
//
LOCUS       DASVMB010000281         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_327696,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000281 DASVMB010000000
VERSION     DASVMB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327696"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VF531_08090"
     CDS             <1..917
                     /locus_tag="VF531_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX7713972.1"
                     /translation="NYRDDPVGYRALSQNLGIINMVIPVSFKVDQYGTIQNNHNPKPF
                     TLARSGGADTLALINNIQGNNFNSGTIHRMLSSSSSRARAINGILRLLVEKGYQGVNI
                     DFENVPANDRYYVTAFFRELAAALRPRNLLVTASLPAKTYNDTSSSHGGAFDYGALAP
                     YLDQLMIMTYDEHYSGGSAGPVASYPWVEKVIQYALRSFPARKIILGIAAYGYDWGWG
                     SGKALNYNAIQSLLRQHQLTPKWDSVSRVPYFKYRSWGIQHQVWYEDRFSTAAKMTLV
                     KKYSLRGVTVWRLGYEDPGIWKTIQQGF"
     gene            complement(1164..2147)
                     /locus_tag="VF531_08095"
     CDS             complement(1164..2147)
                     /locus_tag="VF531_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7713973.1"
                     /translation="MRIFFLGQRQLRMLVILIIGLVFVAGIAVNWTRIARRVIGVRAG
                     VSLDGHLVGGLLPNEVAQVVKGMAAQFNRNPRNATYFLETGEIIPEQTGRTVDVAATV
                     NRICTAAAGSNLGMVVRELPPAITQEYFKPIYHGNENSPRVALAINVAWGEEYIPEYL
                     KIFQAEKVKVTFFLVGTWVKTFPELVRKMAAAGHELANHGMYHGHPDQMGREDLKELI
                     IANTDLIQSVIGKTPVKYFAPPYGEMNSSIVGTAGDLGYRTIMWSVDSIDWKRPAPDV
                     LLERVLSKIEPGGIILLHPTQPTKEALPALIRGLRQKGLEPGTVTSVLQEA"
     gene            complement(2304..3158)
                     /locus_tag="VF531_08100"
     CDS             complement(2304..3158)
                     /locus_tag="VF531_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7713974.1"
                     /translation="MTNQSQSIAVITDSTCDLPREILERFSIHTLPLKVIYPDGEYSD
                     GVDIDAETIYQRMPAEVPKTSMVSMGAALKLFATLREKGIRQVFAIHIASTLSGTVDM
                     IRTVSREFSEMKIEVIDSRSASMGMGFLSYAAAKLVTEGLHFEEIKDRLHHMRERIRI
                     FCCVPALDYLRRGGRIGAVAATVGSLIDIKPIISMDAEGKIFTFDKVRGRKRSIERLI
                     QIIAELGREGKMNIAVMHGAAKDEALKIQEALAKLPSVQEIMLGSIGPVIGIHTGPGL
                     VGVAVQML"
     gene            complement(3182..>4016)
                     /gene="lpxB"
                     /locus_tag="VF531_08105"
     CDS             complement(3182..>4016)
                     /gene="lpxB"
                     /locus_tag="VF531_08105"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7713975.1"
                     /translation="IPVVCMFSPSAWAYGQDRAVKLAERVELLLAVLPFEADFYRKFG
                     TNVTYVGHPLIDRVRPELDPKQYRQQLKLAPGEKLVALLPGSRRQEIQSLLPPMLEAA
                     RLLERDHQIRWLLPVAASLDKTWLDEMIREHKVPVTLLTSGTYDLLAAADAAVISSGT
                     ATLEAAILDTPLVVVYRLSKATLWIYKRMEAQEHKGKPLLVGLPNLIAGREIVPEVLQ
                     ENLTPEQIALALGSILKESNNRRIREELAAIRERIGPPGVMERAATLISKLIAEAGSG
                     N"
BASE COUNT          950 a         1112 c          983 g          971 t
ORIGIN      
        1 aaaactaccg tgatgacccg gttggctatc gggcgctttc acagaatctc ggaattatca
       61 acatggtgat ccctgtctca tttaaagttg accaatatgg gaccatccag aataaccaca
      121 atccaaaacc tttcacattg gcccgttccg gtggcgccga taccctggca ttaatcaata
      181 atattcaggg aaacaacttc aatagcggaa cgatccatcg gatgctctcc agctcaagct
      241 ccagggcccg ggcaatcaat ggtatcctgc ggcttttggt ggaaaaggga tatcagggag
      301 tcaatatcga ttttgaaaat gttcccgcaa atgatcgcta ttatgtgacg gctttcttcc
      361 gggaactggc tgcggcgttg cgccccagga acttgctggt gaccgcctcg cttccggcga
      421 agacctataa cgataccagc tcatcccatg gaggagcttt cgactacggg gcactggcac
      481 cgtatctaga tcagttgatg attatgacct atgacgaaca ttactcggga ggcagcgccg
      541 gaccggtagc ctcatatccc tgggtggaaa aggtcatcca atatgccttg cgttccttcc
      601 cggcccggaa aatcatcctc ggaatagcgg cttacggtta cgattggggt tggggttcag
      661 gcaaggcgct aaattataac gccatccaga gcttgcttcg gcagcatcag cttaccccaa
      721 aatgggattc ggtatccaga gtaccttatt tcaaataccg gagttgggga attcaacatc
      781 aagtctggta cgaggatcga ttcagcaccg ccgcgaagat gaccctggtg aaaaaataca
      841 gcctccgggg agtgacggtc tggcggttgg gttacgagga tccggggatt tggaaaacga
      901 ttcaacaggg gttttaatag gcgtcgcgtc ttgcgggacg cgcggcaact caccccatgt
      961 tttctattag ctttcagtat aaccctacgc ccctctcttt cgaaaaagag aggggtcggg
     1021 ggtgagttgc taataggcaa tactatacgg gtgccatcga tgaggttagc taccccaccc
     1081 ccgaaaactt tttggacttt ttggacttta gactagagta aatcacttcc aatactgttg
     1141 cctcttgcct cttgcctctt gcctcatgcc tcttgcagca ccgaagtcac cgtccccggt
     1201 tccagcccct tttgccgcaa cccacgaatt aacgcaggta atgcttcctt ggtaggttgg
     1261 gtcggatgga gcagaatgat tccgccgggt tcgatcttgg agagaacccg ctccaacaac
     1321 acatcgggag ccggtcgctt ccaatcgatg gaatccaccg accacatgat ggtccggtac
     1381 cccaaatccc ctgccgttcc tacgattgac gaattcattt cgccataagg cggagcgaaa
     1441 tacttcaccg gagtttttcc gataaccgat tgaatcaagt ccgtgttcgc aataatcaat
     1501 tcttttaaat cttctctgcc catctgatcc ggatgtccgt gatacatccc gtgattggcc
     1561 agttcgtggc cggcggcggc catcttccgt accagctcgg gaaaggtttt cacccaggta
     1621 cccacgagaa agaaggttac cttgaccttt tcggcttgga agatctttaa gtactcaggg
     1681 atatattctt caccccaggc tacgttgatg gccaaggcta cccggggtga attttcattg
     1741 ccgtgataga tgggtttgaa gtattcctgg gtaattgcag gggggagttc acgaacgacc
     1801 atgcctagat tggatccggc agcggcggta cagatccggt tgaccgtcgc cgccacatct
     1861 actgtccgcc ctgtctgctc ggggatgatc tctccagtct ccaggaaata ggtggcatta
     1921 cgggggttac ggttaaactg ggcggccatc cccttgacta cttgggcgac ttcattaggt
     1981 aacaatcccc cgaccagatg gccatcaaga cttacacccg cccggacacc gatcacccga
     2041 cgggcgatcc gggtccagtt aactgcgatt cctgctacga ataccagacc gattatcaga
     2101 atcaccaaca tccgcagttg acgttggccc agaaagaaaa ttctcatgcc gccaccccca
     2161 tatacgcgta cacatgcttg ttacgttaca tctattcagg ggtggaacga agtagtactc
     2221 ggtttttcta accccgattc ggccaatttt ttgaaaaagc acagaaaaat tggccgaatc
     2281 gggtgcgcgc acgagtgctc gatttatagc atttgaaccg ccacgcccac caaacccggg
     2341 ccggtatgaa tcccgatcac cggaccgatc gagccgagca tgatctcctg aacactgggg
     2401 agtttggcaa gggcttcctg aatcttcagc gcttcgtcct tggccgcgcc gtgcattaca
     2461 gcgatattca tcttaccttc ccgccccaat tcggctatga tctggatcaa acgctcaatc
     2521 gaacgttttc tgccccgaac cttatcgaat gtaaaaatct ttccctccgc atccatagag
     2581 atgatgggtt taatatcaat caatgagcct accgttgcgg ccaccgctcc gatccgtcca
     2641 ccacggcgca aataatcaag agccggcacg caacagaaga tccggatcct ttcccgcata
     2701 tgatgtaacc tatccttgat ctcctcgaaa tgaaggcctt ccgttactaa cttggcagcc
     2761 gcgtaagata aaaagcccat tcccatcgag gccgatcgag aatcgatcac ttcgatcttc
     2821 atctcgctaa actccctgct gacagtgcgg atcatatcca ccgtaccgct tagtgtactg
     2881 gcaatatgaa tcgcgaaaac ctggcgaatt cctttctcgc gtaaagtggc gaaaagtttc
     2941 aaagccgcac ccatcgatac cattgaggtc ttcggtactt ccgccggcat cctttgataa
     3001 atcgtctcag cgtcaatgtc aaccccgtcg ctatattctc catccgggta tatcacctta
     3061 agaggcaggg tatgaattga aaaacgttcc aggatttctc tggggagatc gcaggtactg
     3121 tcagtaatta cagcgatcga ttgcgattga ttcgtcatgt gatttccttt ctgtatcggt
     3181 tttaattacc ggagccggct tcggcgatca atttgctgat caatgtcgcc gctcgttcca
     3241 tcactcccgg cgggccgatc cgttcccgga ttgcagccaa ttcctcccga atccgccggt
     3301 tattggattc tttcaatatc gaaccgaggg ctaaagcgat ctgttccggg gtcagattct
     3361 cctgaagaac ctcgggcacg atctcacggc ctgcaattag gttgggtagt cccaccaaaa
     3421 gcggttttcc tttgtgttcc tgcgcctcca tccgtttata aatccaaagt gtggctttgg
     3481 aaagccgata gaccacgacc aatggggtat ccaggatcgc cgcctcaagc gtggcggtac
     3541 cggatgagat caccgccgca tccgccgcgg ccagcaggtc ataagtaccg gaggttaaca
     3601 gcgttaccgg aaccttgtgt tcccggatca tttcatcaag ccaagtctta tccagcgagg
     3661 ctgctaccgg caatagccaa cgaatctggt gatcccgttc cagaaggcgg gccgcctcaa
     3721 gcatcggcgg gagtaagctc tgaatctcct gacgcctgct cccgggaagt agcgcaacaa
     3781 gtttttctcc gggggcaagc tttagctgtt gccggtattg tttcggatca agttccggcc
     3841 gaacccggtc aatcaaagga tggcccacat aagttacgtt ggtcccgaat ttacggtaaa
     3901 agtccgcctc aaacgggagt actgccagga gcaactcaac gcgttcagcg agctttaccg
     3961 ctcgatcctg gccgtatgcc caagcggagg gcgaaaacat acagactacc ggaatt
//
LOCUS       DASVMB010000282         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_698183,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000282 DASVMB010000000
VERSION     DASVMB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698183"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF531_08110"
     CDS             complement(<1..636)
                     /locus_tag="VF531_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HEX7713976.1"
                     /translation="MDLDKIVKALSSVPGVSAIALGGSQSRKQADSNSDYDIGLYYDM
                     VNLNLTALGDGLQSLDDNHRDQLLNPPGAWGPWINGGAWITVAGTPVDILLRDVRRVT
                     EVLQDCLAGKITIDYQCGHPFGFVNTIYAAETHFCAKLWQDAAAPLDALKNLLYSEGP
                     YPPMMRRALIQKFFWEAWFSLACGRKAALKGDIHYAVGSAFRAVGAWVEVLF"
     gene            complement(676..1362)
                     /gene="sfsA"
                     /locus_tag="VF531_08115"
     CDS             complement(676..1362)
                     /gene="sfsA"
                     /locus_tag="VF531_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX7713977.1"
                     /translation="MKYPSIVRGVFLDRPNRFIAKVMIDGILETVHVKNTGRCREILR
                     PGAAVILERAGHPGRKTAYSLVAVYKGDLLINIDSQIPNQVVAEALRQGLLPEFGNPV
                     RIAREVVFGNSRFDLYYENESEKGYIEVKGVTLERDGTALFPDAPTERGTKHLLELIE
                     AAKSGYTAAIFFLIQMKGPIRFQPNTLTDPKFAEALLLAKDSGVKVLAYDSVVSKDGI
                     VMGGPVPLDL"
     gene            1602..2051
                     /locus_tag="VF531_08120"
     CDS             1602..2051
                     /locus_tag="VF531_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7713978.1"
                     /translation="MKQSMSSIHEIVARLTRYFVEAGEQNERDHLADISMKQVYYIEM
                     IERLGNPSFGELTKSLGLSKPSITAIVQKLIQGGYIRKDRSVEDKRIFHICLTEKGRQ
                     ICKVHEEMHERIIQDFQKYLSGVEMEQLFAILGKITRGIEDNAGRDS"
BASE COUNT          554 a          559 c          466 g          482 t
ORIGIN      
        1 aaaaagcact tccacccatg cacccaccgc tctgaaagcc gaacccacgg cgtagtgaat
       61 atcccccttt aaagcggcct tacggccgca cgccaaagaa aaccaggctt cccagaagaa
      121 cttttggatg agcgccctcc gcatcatggg aggataagga ccttccgagt aaagcagatt
      181 tttcaaggca tcgagcggag ccgcagcatc ctgccacagc ttggcacaaa aatgggtctc
      241 agctgcgtaa atggtgttga caaagccgaa gggatgcccg cattgataat cgatggtaat
      301 ctttccggcg aggcagtcct gtaacacttc ggtcacccgc cggacatccc ggagcaggat
      361 atccacaggt gtcccggcca ctgtaatcca ggcaccaccg tttatccaag ggccccaggc
      421 accgggagga ttcagcagtt gatcacgatg gttatcatcc aaactttgta agccatctcc
      481 aagagcagtt aagtttagat tcaccatgtc ataataaagc ccaatatcat aatcggaatt
      541 tgaatccgct tgcttccggc tttgcgaccc gcccaaggca atggcggaaa ctccagggac
      601 actcgatagt gctttgacta ttttatctag atccatatca aaatctcatc ccttttgttt
      661 gcctgattac tccattcaca gatcaagcgg caccggtccc cccatcacaa tgccgtcctt
      721 actcacaacc gagtcatagg ccaaaacctt tactccgcta tctttcgcca gcagcaaagc
      781 ctcggcaaat ttcggatccg tgagcgtgtt cggctgaaaa cggatggggc ctttcatctg
      841 gatcaaaaag aagatcgccg ccgtgtaacc tgatttggcg gcttcaatca actccagaag
      901 gtgtttggtt ccccgctccg tcggggcatc cggaaataac gccgtcccgt cccgctccag
      961 agtaaccccc ttgacctcaa tataaccttt ttccgattca ttctcgtaat acaaatcgaa
     1021 acgggagttc ccaaagacca cttccctggc aatccggacc gggttgccga attccggaag
     1081 caggccttgc cgcagcgcct cagcgaccac ctgattcgga atctgggaat cgatgttgat
     1141 caacagatcc cctttataaa ccgccaccaa cgagtaggcg gtcttccgtc ccggatgtcc
     1201 ggcccgttcc aggatgactg ccgccccggg cctgagaatc tcccggcacc ggccggtatt
     1261 cttgacatgg accgtctcca aaatgccgtc gatcatgacc ttggcgataa aacggttggg
     1321 cctatccaaa aagacccccc gcacaatact ggggtatttc atatgttcgc ttccatccat
     1381 taaccggcta atgcggtact tgatcttact atagcatctc cccgtttttt tacaacccct
     1441 tggtacggat agcggaaact tttcatacga accactttca attaccgtca aagtaacgaa
     1501 ttgtttgaat gggaataacc ggttgacaag atttcctatt tcacttataa ttagtttgta
     1561 catatgaact aattatcata ttaaaccagg agattgagca aatgaagcaa tctatgagct
     1621 ccattcatga aatcgtcgcc cgtttgaccc ggtattttgt tgaagccggg gaacaaaacg
     1681 agcgggatca tttggcggat atctccatga agcaggtata ttatatcgaa atgatagagc
     1741 gcctgggaaa tcccagcttc ggagagctca ccaaatcctt gggattaagt aaaccctcga
     1801 tcactgccat tgttcaaaag ctgattcagg gaggatatat acgaaaagac cggtccgtgg
     1861 aggacaaacg gatctttcat atttgcctga ctgaaaaagg ccgtcagata tgtaaagtcc
     1921 acgaggaaat gcatgagcgg atcattcaag acttccaaaa gtacctcagc ggtgttgaaa
     1981 tggaacaact tttcgcaata ctcggcaaga taacccgggg tatagaggac aacgccggac
     2041 gcgattcata aaggggggta t
//
LOCUS       DASVMB010000283         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_401299,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000283 DASVMB010000000
VERSION     DASVMB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401299"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF531_08125"
     CDS             <1..252
                     /locus_tag="VF531_08125"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEX7713979.1"
                     /translation="ALFSRDNIQPVVAGELPSDIQNLTMRHILNHETILKAAINRDKE
                     LAFHAFSNDPQVTLSYAEAQELFDQMLAATKEYLSGWNL"
     gene            518..1504
                     /locus_tag="VF531_08130"
     CDS             518..1504
                     /locus_tag="VF531_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7713980.1"
                     /translation="MNDNVQNVAMQEVAVREVASKIRANIGRVLVGKEAAVELALVAL
                     GCEGHLLFEDVPGVGKTMLARALAVSLGLSFSRIQCTPDLLPSDVTGMMVFNPKNAEF
                     QLRSGPIMAQIVLVDEINRATPRTQSSMLEAMGERQVTIDGRAYSLPQPFLVLATENP
                     VEFEGTFPLPEAELDRFLLRVSLGYPSATEEMQILTRLEGEHPIHTLGSVVTPEELRD
                     FTALRREVYMEDSLRSYLVELVARTRSHPDLELGVSPRGTVALFDTARMLAALRGRNF
                     VSPEDIKYLAPYVLAHRVKVGAEAAVRGVTATSVIAEIVAGTPVPTEAAFNE"
     gene            1497..2684
                     /locus_tag="VF531_08135"
     CDS             1497..2684
                     /locus_tag="VF531_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7713981.1"
                     /translation="MSSLLVTLVLLGLIALATGIPWIYRLCYLGLGLAVGVRWWSRRS
                     REGIGLERDLTRSTGFAGTDLSLKLELKNTSRMPLFWGRVSQSFPEPLGAEQKEALIW
                     LKPRGRTVLDFTFRAERRGVYPLPQTRFSWGDPWGLTENNLILNSDARIVVYPPLRPI
                     IGLEPIRHLPWGRHPVFFGLYEDPARLRGCRDYTTADSFKRIHWPNLARTGKLQVKEW
                     ETTLQAGWTIFLNLNEPDYPVDEWSPLSELGIELAAALAHGFSSEPVGFYCNGRGMGA
                     AEGTVLKLPPKQGIHREQQILFYLAGVEPRPGMEFSVLCREARSLAAGSCLLWITPDV
                     GADLVRQVGILRRLGYYPVVLWLRARQGEDGQACQAQLDQAEIQRYIVTRRREDDAIY
                     ITR"
     gene            2659..4188
                     /locus_tag="VF531_08140"
     CDS             2659..4188
                     /locus_tag="VF531_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX7713982.1"
                     /translation="MMPSILPDKTGEEKPPLLGTVILSGYAATVGLYLAVLTLPGLLR
                     PAPWVFVLSILVFSLFSALYRIGTWNLRLPMLWHGFVAAIALWLGIAGWVFFRGGPVA
                     ACFSRPFGVVAEEWEWLLVVVLTWVLTVWFGDLLRRLARVPQHPAVPKPSKQIWEDAD
                     NRISLVPWEDAWKSWRGSLCGVIILEVLGWIVVRGVSPQSIRPSAGWLLLLETGIGLG
                     LWSLGYFYYQRAFWRSDKLVPGSGLSRNWLRFCAGFIVILVCAALVLPATVPSLNWDF
                     LGRLLAKLEISGDATVPESILSRNPGRVQETMPPRDFSIISLIMGLLYQVILPLFCLL
                     FIIGVIGYLFFRFCAGEVDKIKGLRGALIKFYLFWRGLFTRCLRSVTEYLGMKPLSQD
                     SVSTEARSRKKHSSLEWGRGPRAIIRRGYYRLIQFARESGLKWRHSRTPEELAADLAR
                     ILPQDGETISTLTEDYLAARYGPEPPAAGRAGRFERLRRVVLNHLKGKGKSNDSGDEN
                     R"
     gene            4163..4759
                     /locus_tag="VF531_08145"
     CDS             4163..4759
                     /locus_tag="VF531_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713983.1"
                     /translation="MIPETKTDNECKKPSRIEASPEFTFSDQGVWGVLLKLRLAWGWL
                     LLLATVVFAVILQMGAYQLIPWLGAAITAAGFGSSIWILKRNRKIVTPACLALSWNWA
                     GCYLGMGILMFGIYAKTRYPLFIPPVIATGMGLSSWITASIYKKRLQSGLAFSWWCGA
                     LVMWVVGTDYTVLFLGGLLIGLELGPDWLLYRRRHRAS"
     gene            4867..5340
                     /locus_tag="VF531_08150"
     CDS             4867..5340
                     /locus_tag="VF531_08150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012581817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7713984.1"
                     /translation="MSEEDFQEFLQPAIAEYAEEKVKAGTWAESEALQKSRDSFDGLL
                     PSGMETQNQYLFVIIDSGSGNKVGYFWFQLIERLFGREAFIYDFKILEEFRGRSYGHQ
                     TMTAMDEMAKSMSIAKISLHVFAHNQIARKLYEKSGYHETDINMSKWIEFNQIDC"
     gene            5439..>6246
                     /locus_tag="VF531_08155"
     CDS             5439..>6246
                     /locus_tag="VF531_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713985.1"
                     /translation="MNEERIINFERANRIAILVGSIALFLGGLPQIFVENFRLLDLLI
                     IWLLGAIVFIPAFIAYIRKRDSLLVRYFICCGSFVIIYSILYVQKGVLDNLFWLPVCL
                     VASSLFFDVQLTIVTTTLEVLGTIALYVLNQPLFFPDLDLPKFIVFCITMAVTGTVLA
                     FLGQFGSKLILGARRSYQETLGLNERLEAVVQSIERNSDIINLTIAELGKDSITAKEE
                     TQNITVAIEQFNKVVENLAEGTSSSITSLASIENHIKEIADNSEEMKENSR"
BASE COUNT         1382 a         1457 c         1767 g         1640 t
ORIGIN      
        1 gccctgttct cacgggacaa tattcagccg gttgtggccg gagaattgcc ttccgatatc
       61 cagaacctga ccatgcggca tattctgaat cacgaaacca ttcttaaggc agcaatcaac
      121 cgtgacaagg aactggcatt ccatgccttc agcaatgatc cgcaggtgac gctctcctac
      181 gccgaggcgc aggagctttt tgaccagatg ctggctgcaa ccaaggagta cttgtccggc
      241 tggaatctgt aagcagataa caataatcca aaatccaagg gcttcctttg caggaagcct
      301 tttttttgta tcgaattcag aatagatatc ccattcagca tttgttgcag aacaaccact
      361 gttaaagaac tgcaacgttt agctaatacc cttaacggat gaggtcgcct ttaggcattc
      421 ataatgtctg gttgatacat ggcgaaacag ctatattgat atattggctt agcaaactag
      481 atcgtcatga gataatctga aaattggagg aaccccgatg aatgacaacg tgcagaacgt
      541 ggccatgcag gaagtggctg tgcgagaagt ggcttctaaa attcgcgcca acatcggccg
      601 ggttttggtg ggtaaagagg cggcagtcga attggccctg gtagccctgg gatgtgaggg
      661 acacttgctt tttgaggatg ttcccggtgt cggtaagacg atgctcgccc gggcgctggc
      721 cgtctctttg gggctgagct ttagccggat ccagtgcact cccgatctgc tcccctccga
      781 tgtcaccgga atgatggttt ttaatccgaa aaacgccgaa tttcagctgc gctccggtcc
      841 aatcatggcc cagatcgttt tggtggatga gatcaatcgc gctacgccgc gtacccagtc
      901 cagcatgctt gaggcgatgg gggaacggca ggtgaccatc gacggccggg catactcatt
      961 gccgcagccc tttttggttt tagccacgga gaacccggta gagttcgagg gaacctttcc
     1021 cttgcccgag gcggagttgg atcggttttt gctccgggtt tccctgggat accccagtgc
     1081 caccgaggag atgcagatcc tcaccaggct tgagggggag catcccatcc atactttggg
     1141 ttcggtagtc actcccgagg agctacgcga ttttaccgcg ttgcgccgcg aggtatatat
     1201 ggaagacagc ttacgaagct atttggtgga gttagtcgcc cggacccgtt cccatcccga
     1261 tctggagttg ggggtcagcc cccgcggaac ggtggctctc tttgatactg cccggatgct
     1321 tgcagcactt cggggacgta actttgtttc cccggaggat atcaaatatc tggctcccta
     1381 tgttctcgcc caccgggtaa aggttggggc ggaagcggcg gtaagaggag tcacagccac
     1441 atccgtgatt gcggagatcg ttgccgggac tccggtaccg accgaggcgg catttaatga
     1501 gtagcctgtt ggtaactctg gtcctgctgg ggttgatagc cttagcgacc ggtattccct
     1561 ggatttatcg tctgtgttat ttgggattgg gattggcagt tggggtccgg tggtggtcga
     1621 ggcggagccg ggagggaata ggcttggaac gggatctgac ccgcagcacc ggttttgcag
     1681 gaaccgatct cagcctgaaa cttgaactca aaaacacctc gcggatgcca cttttttggg
     1741 gacgggtcag ccagtctttt ccggaacccc tcggtgccga acaaaaggaa gccttgatct
     1801 ggttaaaacc ccgaggccgg acggttttgg attttacatt ccgggccgag cggcgcggtg
     1861 tgtatccctt gccccaaacc agattctcct ggggggatcc ctggggcttg acggagaata
     1921 atctgatttt aaactccgat gcgagaatcg tcgtttaccc gcctctccga cctatcatcg
     1981 gtttagaacc cattcggcat ctaccctggg gacggcatcc cgtctttttc ggtttatatg
     2041 aggatcccgc ccggctgcgg gggtgccggg attataccac ggcggacagt tttaaaagga
     2101 ttcactggcc gaatctggcc cgtaccggta agcttcaggt gaaagagtgg gagaccactc
     2161 tccaggcagg ctggacaata ttcctcaatc taaatgaacc cgactatccg gtggatgagt
     2221 ggtctccttt gagtgagctg gggatcgagt tggccgccgc gcttgcccat ggtttttctt
     2281 cggaaccggt gggcttctat tgcaacggcc gggggatggg cgctgccgaa gggaccgtct
     2341 taaaactccc gccaaagcag gggatccatc gggagcaaca gatcctgttt tacttagccg
     2401 gggtggaacc ccggccggga atggaattca gtgtcttgtg ccgtgaagcc cgaagtctag
     2461 cggcggggtc gtgcctgctt tggatcaccc cggacgttgg cgccgatctg gtgcgacagg
     2521 tcgggatttt acggcgactt ggttattatc cggtggttct ttggcttcgc gcccgacagg
     2581 gagaggatgg ccaagcctgc caggcccaac tggatcaagc ggaaatccag cgttatattg
     2641 tgacgagaag gcgggaagat gatgccatct atattacccg ataaaacagg ggaagagaag
     2701 ccaccgttat taggaacggt gatcctatcg ggttacgccg caaccgtagg gttgtatctt
     2761 gccgtattga cgttacccgg gttgttgcga cccgccccct gggtatttgt cctgagtatt
     2821 ttggttttca gtttattcag cgcactttac cggataggca catggaattt gcggctgccc
     2881 atgctttggc atggtttcgt cgccgccatc gcgttgtggc tgggaatcgc aggttgggta
     2941 tttttccggg gcggtccggt agcggcttgt tttagccggc cctttggggt tgtggcggaa
     3001 gaatgggagt ggctgttggt tgtagtcctt acctgggtac tgactgtatg gtttggtgat
     3061 ctattacgcc gcttggcccg ggttcctcag catccggctg ttcccaagcc ttcgaaacag
     3121 atctgggaag atgccgataa ccggatatcc cttgtcccct gggaagatgc ctggaaaagc
     3181 tggcggggtt ccctgtgcgg ggtcattatc ctagaagtcc ttggatggat agtggtgagg
     3241 ggagtcagcc cccaatcgat cagaccgtcg gccggatggt tgttgcttct ggagacggga
     3301 atcgggttgg gtttgtggag tctgggctat ttctactacc aacgggcatt ttggcgaagc
     3361 gacaagcttg ttcccggttc cggtctaagc cgtaattggc tccggttttg cgccggtttc
     3421 atcgtgattt tggtatgtgc ggcgctggta ttgcccgcta ctgttcccag tctcaactgg
     3481 gattttcttg gaaggttgct ggccaagttg gaaatatcgg gggatgcgac ggtcccggaa
     3541 tcgattttat caaggaatcc ggggcgggtg caggaaacca tgcctcctcg ggatttctca
     3601 atcatttccc tgatcatggg cctcttgtat caggtaattc ttcccttgtt ctgtttacta
     3661 ttcatcattg gggtcatcgg ttatcttttc ttccggtttt gcgccggtga agtcgataaa
     3721 atcaaaggat tgcgcggggc gctcatcaaa ttttatcttt tctggagggg tctattcacc
     3781 cgttgcctaa gatcggtaac ggaatacctt ggaatgaaac ctttgtccca ggattcagtt
     3841 tccacagagg cacgatcccg caaaaagcat tcaagtttgg aatggggccg ggggccgcgg
     3901 gccattatcc ggcgcggcta ttaccgttta attcaatttg cccgggaaag cggtctaaaa
     3961 tggcgtcact cccggacccc ggaggaatta gccgccgatc tggcccggat tttaccacag
     4021 gatggggaaa ccatttctac gctgacggag gattatcttg cggcaaggta tggacctgag
     4081 cctccggccg cgggcagggc cggacgtttt gaacgattac gccgggtggt gctcaatcat
     4141 ttgaaaggaa aaggtaaatc caatgattcc ggagacgaaa acagataatg aatgtaaaaa
     4201 accaagccgg atcgaagcgt caccggaatt tacgttctcc gatcaagggg tatggggagt
     4261 cttgctcaaa cttcgcctgg cctggggttg gctactgctt ctggccacgg tggttttcgc
     4321 cgttatcctg caaatgggtg cctatcagct tattccgtgg ctcggggcag ctattacggc
     4381 agcagggttc ggatcttcca tttggatttt gaagcgcaac cgaaagattg tcactccggc
     4441 atgtctggca cttagctgga attgggccgg ttgttatctc ggaatgggga tcctgatgtt
     4501 tgggatatat gccaagactc gatacccgct atttattcca cctgtcattg ctaccggaat
     4561 gggacttagc agttggatca ccgcgtccat ttacaagaaa cggcttcaga gcggtctggc
     4621 attttcatgg tggtgcggtg cgctggtaat gtgggtcgtg ggaacagact acaccgtgtt
     4681 gttcctgggg gggctcttaa tcgggcttga gcttgggccg gattggctgc tctatcgtcg
     4741 taggcatcgc gcttcctgag gcgcgcggca tcagccctca cccattttgg cagcctctca
     4801 atatcgctta ctcagatgtg ggaggaaact ataagggggt aaatggtatt ggttcgattt
     4861 gaaaaaatga gtgaggaaga ctttcaagaa tttcttcaac cggcaatcgc ggaatatgcc
     4921 gaggaaaaag taaaagccgg tacgtgggct gagagcgaag cccttcagaa gtctcgagac
     4981 agttttgatg gactgcttcc cagtggaatg gagacccaaa atcaatattt attcgtaatt
     5041 attgattccg gctcaggaaa caaagtcggg tatttctggt tccagctaat tgaaagactg
     5101 tttggccggg aggctttcat ctatgatttt aaaatccttg aggaatttcg cggccggagt
     5161 tatggtcacc agacgatgac tgccatggat gaaatggcca aaagtatgag tatcgccaag
     5221 atttcattgc atgtattcgc gcataatcag atcgcgcgca agctatatga aaagtcaggc
     5281 tatcatgaaa cggatatcaa tatgtcaaaa tggatagaat ttaatcagat agattgttaa
     5341 ctaaagtttt ttgaaagaag ttcgataaca atctatatat attgtccatt atcgggagaa
     5401 tattcggccg tatagaatga gccaaagaag gtgcgccaat gaatgaagag agaattatca
     5461 attttgagcg ggccaatcga attgcgatcc tggttggatc aatcgccctg tttttaggtg
     5521 gtttgcccca gatctttgtg gaaaattttc gacttttaga tcttctcatc atttggctgt
     5581 tgggggccat cgtattcatc cccgcattca tcgcctatat ccgtaaaaga gacagcctgt
     5641 tggttcgtta tttcatttgt tgcggatctt ttgtgattat ctatagcatc ctgtatgtcc
     5701 aaaagggagt gctggacaat cttttttggt tgccggtctg tctggttgcc agctcacttt
     5761 ttttcgatgt acagcttacc attgtcacta ctacacttga ggtgcttgga accatagccc
     5821 tttatgtcct gaatcaaccg ttgttctttc cggatctgga tctcccgaag ttcatcgtct
     5881 tttgtatcac tatggcggtt accggcacgg tattggcgtt tttgggacag ttcgggagta
     5941 aattgatttt aggtgcccga aggagctatc aggaaacgtt gggtcttaat gaaagattgg
     6001 aagcggtcgt tcaaagtatt gaaaggaact cagacattat caacctaacc atcgcggaac
     6061 tcgggaagga ttcaatcacc gccaaggaag aaacccaaaa catcacggtt gccattgaac
     6121 agttcaataa ggttgttgag aatctggcgg aggggacctc gtcctccatt acttcactgg
     6181 catcgattga aaatcacatc aaggagattg cagataattc cgaggaaatg aaggaaaatt
     6241 cgcgta
//
LOCUS       DASVMB010000284         6533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_401642,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000284 DASVMB010000000
VERSION     DASVMB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401642"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF531_08160"
     CDS             <1..282
                     /locus_tag="VF531_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX7713986.1"
                     /translation="SKTASKYNAVKYLRDHYPFDRIVGFGDNFNDMPRFDACDECYAV
                     FNAVEELRNKATGVIGDNNSHGVARFIAEREGLGVGLRSPLPFPIKVEL"
     gene            549..1073
                     /locus_tag="VF531_08165"
     CDS             549..1073
                     /locus_tag="VF531_08165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713987.1"
                     /translation="MESLTIRKATASDLDFVENIYNESIDWLINQNIHQWQRGVYPTR
                     DTALEAFRDDCLYCCFIHDRMVATFIINESQPSQYQTLNWKYNQGQPLVLHTLVVRPG
                     EAGQGIGRAIVEYVIVQAKQNGHSCIRLDAFPDNQAAIRLYQAFGFEYVGKVFFDIKE
                     PSYEWYDCYEKKIS"
     gene            1104..2144
                     /locus_tag="VF531_08170"
     CDS             1104..2144
                     /locus_tag="VF531_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7713988.1"
                     /translation="MVFFETTQNSKEFAVLGLGEVLLRLSPPNKERILAGEVFEKRAG
                     GSELNVVSGISLLGLRTGMITKLPGNEIGKYINNKIRFSGVSDDYIIFDDHPGARLGI
                     YYYESGAAPRKPTVVYDRANSSFTSLKLEEIDPEVYQKTAVFHVSGITLALGQAIRET
                     TRTMIQKFKAQGSLISFDVNFRASLWSEAEARTEISKILPEIDILFISEETSRKMFGK
                     QGSLEDIMKSYAAEYGCKLVATTQRQVISPSKHNWNSLIYSKAEDRFFQEEPYREIDV
                     VDRIGSGDAYLAGVLFGLLKYNNVQSALEFGNAMAAIKNTIAGDMPVSDFQEIERIIR
                     DHQVKDTSELNR"
     gene            2344..3639
                     /locus_tag="VF531_08175"
     CDS             2344..3639
                     /locus_tag="VF531_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7713989.1"
                     /translation="MERKIVNRLKLWKDSSRRMPLILYGARQVGKTYTILSFGKEHYK
                     NTVYFNFEDSTELTNIFERDLNPARIIKELSAKSGQTILKSETLIVFDEIQACERALT
                     SLKYFCENAPEYHIMAAGSMLGVALNRQKYSFPVGKVEIMTLYPLDFEEFLWALGKKD
                     LAGLIRNSFESHVSLSLHDTAMDLFRMYLVIGGMPRAVLEYIEKQDFNFVLAAQKTIN
                     HAYIADMAKYATPMETTRILAAFNSISAQLAKDNRKFQYKVIKSGARAYEYETPLEWL
                     KASGVIMKCHKVNEGRIPLTAYMDGSFFKIYMTDTGLLCSKFGVPPNAVLVETPSFEG
                     FKGALAENYVCSALTMNGYNPFYWESQGKAEIDFVIQDRGGGIIPVEVKSGDHVKAKS
                     LAQFVGRYQCPYAIRISARNFGFENGIQAVPLYAAFCIN"
     gene            3662..4153
                     /locus_tag="VF531_08180"
     CDS             3662..4153
                     /locus_tag="VF531_08180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7713990.1"
                     /translation="MIVYLATMDDYQAWLDFTERVLPIFGIELSTDEGYQKALEKNIR
                     RGTAFCIKADDAPDRKIIGGMLLSPTQKPVYQIGWFAVDKDFMHRGIGTTLFKFMMTR
                     IEPPAEVFVDTFTASDPHGISARNFYAKMGMVPAEIIEGDFPHGRQCQIYRKKLEAFE
                     PMV"
     gene            4227..4958
                     /locus_tag="VF531_08185"
     CDS             4227..4958
                     /locus_tag="VF531_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7713991.1"
                     /translation="MKVNATDLKNHLGKYLRLSVREEILITSNGKVIAKLSGYEEMNQ
                     DNAGFNLQEQAKAYEIYPRKASFEEFQKLTENSDERYEYIDGEIYFLASPKTLHQKIL
                     SELHVIFYNWFQGKPCRPMLAPYDITLKRNPENINVVQPDLMVICDLEERLNEKDYYM
                     GVPTLVVEIISETTRSKDFVKKLDLHMETGVSEYWIVNPLNHEVSVYLFRENNVAKNM
                     TFKNNETVSSFYFPGLKVSLDQIFG"
     gene            4986..5777
                     /locus_tag="VF531_08190"
     CDS             4986..5777
                     /locus_tag="VF531_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX7713992.1"
                     /translation="MALIHCNFFSEVLGLSTSMYVILPQATQGQIGMTGQAGAHKHPT
                     LYLLHGLSDDHTIWLRRTSIERYVAPLGLAVVMPEVGRSFYTDMAAGGKYWTFISEEL
                     PEIAQSFFNLSDRREENFAAGLSMGGYGAFKLGLAKPDQFCAAASLSGAVDLESLLAL
                     AQPNEEKEWRNVFGDLDKVPGSTNDLFFLARKLAESGRLQPKLFQWCGTEDFLYKGNI
                     RFRDYARELNLDLTYEEGPGDHQWLYWDQQIQRVLEWLPFPQKEA"
     gene            6009..>6533
                     /locus_tag="VF531_08195"
     CDS             6009..>6533
                     /locus_tag="VF531_08195"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7713993.1"
                     /translation="MTNVRLYGSPPYKAAVVHGGPGALGTVAAIARELAKDFGILEPL
                     QTQGTIAALLEELDEVIATHCGKPISLIGHSWGAWLVFMYAARYPQRVKKIILVGSGP
                     FETHYAKAISPNRMRHLSTEEQKEYNALITALNSKRENDSDRLMGRLGELVDKTDNYC
                     TVEIESDRIDQLPAD"
BASE COUNT         1813 a         1311 c         1701 g         1708 t
ORIGIN      
        1 agcaaaaccg catcaaagta caatgcggtt aaatatcttc gggatcatta cccttttgac
       61 agaatcgtcg gcttcggtga taatttcaac gatatgccgc gctttgatgc atgtgatgag
      121 tgctatgcgg tattcaatgc agttgaagaa ttaaggaata aagctaccgg agtaatcggt
      181 gataataatt cccatggtgt cgccaggttc attgccgaga gggaagggtt aggggttgga
      241 ctacgaagcc cgttaccctt tcccattaaa gtggagttgt aatcacaact cttaagaaaa
      301 cggtgtcacc gttttcaggg aactggctag aatttcgggt ccataggctg ggacaaaact
      361 acgaagtcca accggcaata gatcttcttt aaataattgt gctctactat aataaacgaa
      421 cgataccggt tttaaccggt aagaattatt acagccactg gttttaacca gtggcctgag
      481 atgcaaaggt ttatctgagt taaatagatg gccatcaaaa attttgagga attggagaaa
      541 acgttttaat ggagagcctg acaatccgaa aggccactgc gagtgatctg gatttcgttg
      601 aaaatatcta taacgagtca atcgattggc taattaacca aaatatccat caatggcaga
      661 gaggcgtcta tccgacccgc gatacggcgt tggaagcgtt ccgggatgat tgtctttatt
      721 gctgctttat tcatgataga atggtagcga cctttattat caatgaaagt caaccttccc
      781 aatatcagac cctgaactgg aaatataatc agggacaacc cctggtcttg catactttag
      841 tggttaggcc cggcgaggcc gggcagggaa tcggcagggc gattgtggag tatgtgattg
      901 tgcaagcgaa gcaaaatggt cattcttgta tcaggcttga cgcgttcccg gataaccaag
      961 ccgcaattcg tttatatcag gcatttgggt ttgaatatgt gggaaaggta ttctttgata
     1021 tcaaggaacc gtcttatgaa tggtacgatt gctatgaaaa gaaaatttct taacaaaaat
     1081 aatttcgtga taaggagaat aaaatggttt tttttgagac aactcagaat tccaaagaat
     1141 ttgcagtgtt gggactaggg gaagtgctgt tacgtctttc gccgccgaat aaagaacgga
     1201 ttctggcagg cgaggttttt gaaaagcggg ccggcgggtc cgaactgaat gtggtttccg
     1261 gaatctcgct cctgggactc aggaccggga tgataacgaa gttgccgggt aatgagattg
     1321 gaaagtacat taacaacaag attcggttta gcggggtcag cgacgattac attattttcg
     1381 acgatcaccc cggagcccgt ttgggaattt actactacga aagcggcgcc gcgccacgca
     1441 agccgacagt ggtgtatgat cgggcgaatt catcatttac ttcattgaag ttggaagaga
     1501 ttgatcccga ggtctatcag aaaactgcgg tatttcatgt gagcggcatt accctggctc
     1561 tgggtcaagc catccgggaa accacccgga ccatgattca gaagtttaag gcccagggca
     1621 gtctgatatc gtttgatgtg aactttcggg cttcattatg gagcgaagcg gaagcccgga
     1681 cggaaatttc caaaattttg cccgagatcg acatcttgtt tatctcggag gagacttccc
     1741 gcaagatgtt tgggaaacag ggatccctgg aagatatcat gaaaagttat gcggcagaat
     1801 acggctgtaa attggtggcg acgacccagc gtcaagtgat aagtccctcc aaacataatt
     1861 ggaattcctt aatctactcc aaggccgagg atcgcttttt tcaagaggag ccataccggg
     1921 agatcgatgt ggttgaccgg atcggcagcg gcgatgcata tcttgcggga gtgctgtttg
     1981 gattattgaa atataataat gtccagtcgg cgctggaatt cgggaatgca atggccgcca
     2041 ttaagaacac gattgccggc gatatgccgg tcagcgactt ccaggagata gagcggatta
     2101 tccgggatca tcaggtgaag gatacgagcg agctgaaccg gtaataaagc atagttattc
     2161 gttagtggtt attagttact tgttaaaaga aaagagcaaa atctaatgct ctttttttgt
     2221 tatttgatag atgggtgact ggcgtgaaaa agcgggagta tgaattaact gggaatatat
     2281 ttataaaagt tgtatatgaa tatcacagta taggttataa taagtgtata ggagtggcac
     2341 ttaatggaac gaaaaatcgt aaatagatta aaactctgga aagattccag ccggagaatg
     2401 ccgctcattt tgtatggagc cagacaggtt ggaaaaacat atacgatcct ctcttttggc
     2461 aaagaacatt ataaaaatac ggtgtatttt aatttcgaag atagtaccga gttgaccaat
     2521 atctttgagc gggatttaaa tccggcgcgg attatcaagg agctttccgc gaaatccggt
     2581 caaaccatat taaaaagtga aacattgata gtctttgatg aaattcaagc ctgtgagcgg
     2641 gcgctgacgt cgctcaagta tttctgcgaa aatgcgccgg agtatcatat tatggctgcc
     2701 ggtagtatgt taggcgttgc gctaaatcgt caaaaatatt cttttccggt aggcaaggtt
     2761 gaaataatga ccttgtatcc gctagatttc gaagaatttt tatgggcatt gggaaaaaaa
     2821 gatttggcgg ggcttatccg gaattccttt gagtctcatg tatcattatc attacacgat
     2881 acagccatgg atctttttcg gatgtacttg gtcatcgggg gaatgcccag ggcggtattg
     2941 gagtatatcg agaaacagga ttttaatttt gttttagcgg cacaaaagac aattaatcac
     3001 gcttatatcg ccgatatggc gaaatacgct acgcccatgg aaacaaccag gattttagca
     3061 gcttttaatt ccatatcggc tcaactcgcc aaggataacc ggaaatttca atataaagtc
     3121 atcaaaagcg gggccagggc ttatgaatat gagaccccgc tggaatggct caaagcatcc
     3181 ggggtgatta tgaaatgcca taaagtgaat gaggggagaa tacctttaac cgcgtatatg
     3241 gatggttcct tttttaagat atatatgacc gataccgggt tgctttgctc caagttcggt
     3301 gtgccgccca atgcagtgct ggttgaaaca ccgagttttg aggggtttaa aggcgctttg
     3361 gcggaaaatt atgtgtgctc ggcattaacc atgaacggct acaacccttt ttattgggaa
     3421 tcacagggta aagctgagat tgatttcgta atccaggatc ggggcggggg aattattccg
     3481 gtagaagtta agtccggtga tcatgtgaaa gctaagagtt tagcccagtt tgtcgggaga
     3541 tatcagtgcc cgtatgctat ccggatatcc gccagaaatt ttggtttcga gaatgggatt
     3601 caggcggtac cgctgtatgc ggcgttttgt ataaattagg ggttatccga ggtgatgaat
     3661 tgtgatagtg tatctggcaa caatggacga ttatcaagca tggctcgatt ttacagaacg
     3721 ggtgctgccg atcttcggaa ttgaacttag tacggacgaa ggctatcaaa aagctttgga
     3781 aaagaacatc cggcgcggga ctgcgttttg tatcaaagca gacgatgcgc ctgacagaaa
     3841 aatcatcggc ggcatgcttc tttctcccac ccaaaagccg gtctatcaaa tcggctggtt
     3901 cgcggtggat aaagatttta tgcatcgggg aatcgggact acattgttta agtttatgat
     3961 gacgcggatc gaaccaccgg ctgaagtgtt cgtagatact tttaccgcaa gtgatcctca
     4021 cggaatatcg gcacgaaatt tctatgccaa aatgggaatg gttccggcgg agattattga
     4081 gggcgatttt ccccatggcc gtcaatgcca aatctatcgc aaaaaacttg aggcttttga
     4141 gccgatggtt tgactgaata atcataattc tatataatca aattagctat tttggctaaa
     4201 gcggtacatg gaatggaggc gattggatga aagtgaacgc aaccgacctc aaaaaccatt
     4261 tgggtaaata cctgaggctg tctgtccggg aagaaatctt gattaccagc aacggcaagg
     4321 taatcgctaa attatccggg tatgaagaga tgaatcagga taatgccggc ttcaatcttc
     4381 aagaacaagc gaaagcctat gaaatatacc cacgaaaagc ttcttttgaa gaatttcaaa
     4441 aattaaccga gaatagtgat gagcgctatg aatatatcga cggcgaaatt tattttctag
     4501 cttcccctaa gacactccat cagaaaatcc ttagcgagct tcatgttatc ttttataact
     4561 ggtttcaagg gaagccatgc cgtccgatgc tggcacctta tgatattacg ctgaaaagaa
     4621 atcccgaaaa catcaacgtt gtccagccgg atttgatggt catctgtgat ctggaagaac
     4681 gcctaaatga aaaggattat tatatgggtg tcccgacact ggtggtggag attatctccg
     4741 aaaccacccg cagcaaagat tttgtcaaga aactggatct gcatatggag accggagttt
     4801 ctgaatattg gattgtcaac ccattaaatc atgaagtaag cgtatatctg tttcgggaaa
     4861 ataatgtcgc caaaaatatg acctttaaaa acaatgaaac cgtgtcgtct ttttattttc
     4921 cagggttaaa agtaagtttg gaccagatat tcgggtagcc aatcaatatc cgaggaggcc
     4981 tttttatggc gctgattcat tgcaactttt tttccgaggt tttaggatta tccacttcca
     5041 tgtatgtgat cctgccccag gcgacccagg gacagattgg tatgaccggc caggcgggcg
     5101 cccacaagca tcctactctg tacttactac atgggctatc ggatgatcac accatctggc
     5161 tgcgccggac ttccatcgaa cgttacgtgg cgccgctggg gctggcggtg gtcatgccgg
     5221 aggtgggacg gagcttttat acggatatgg cggccggcgg aaaatattgg acctttatca
     5281 gcgaagaatt gccggagatc gcccaaagtt tcttcaatct ttcggatcgg cgggaggaga
     5341 atttcgcggc cggtctttcc atgggcgggt atggcgcttt caaacttggt ctggctaaac
     5401 cggatcaatt ctgcgctgcg gcaagcctgt ccggagcggt ggatctcgag agtttattgg
     5461 cattagccca gcccaacgaa gaaaaggagt ggcggaatgt cttcggtgat ctggataaag
     5521 ttccgggatc aaccaatgat ctgtttttcc tcgcccggaa gttagcggag tccggtcgtc
     5581 ttcagcctaa gttgtttcaa tggtgcggta ccgaggattt cctgtataaa gggaacatcc
     5641 ggttccggga ttacgccagg gagttgaatt tggatctgac ctatgaagag gggcccgggg
     5701 atcatcagtg gctatattgg gatcagcaga ttcagcgggt tttggaatgg ctgccgttcc
     5761 cccaaaaaga ggcttaagat cacagatatt gggattaatg cggtgatcct ttgccgtcaa
     5821 ccgggtcgta aacgacccgg ctacaaggtg taagccttcc atggctaatc cgggcagaat
     5881 atttcatccc aacccagggc aaggcccttt caacttgtaa ccggaggcgc gtgcgcatat
     5941 gcgcgaagca tccggtcggc atccccatat gccaggatgg attattgttt gaaacggaga
     6001 acgaatggat gacgaacgtc cgattgtatg gtagcccgcc ttacaaggcg gcggtagttc
     6061 acggcgggcc gggggctttg ggaacggtgg cggcgatagc ccgggagttg gcgaaggatt
     6121 ttggaatcct cgagccgcta cagacccaag gaaccattgc ggcactcctt gaggaattgg
     6181 atgaggtcat cgctactcat tgtggcaagc ccatatcatt gatcggccat tcctggggcg
     6241 cttggctcgt cttcatgtac gccgcccgat accctcaaag ggttaaaaaa atcatcctgg
     6301 tcggtagcgg accttttgaa acgcattacg ctaaagctat tagcccgaac cgtatgagac
     6361 acctgtctac tgaagagcaa aaagagtata atgcactaat tacagccttg aactccaaaa
     6421 gggaaaatga ttcggatcgg ttgatgggga gattgggcga gctggtggac aaaaccgata
     6481 actattgtac cgtggaaatc gagagcgacc ggattgatca gctgcccgcg gac
//
LOCUS       DASVMB010000285         8015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_698567,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000285 DASVMB010000000
VERSION     DASVMB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698567"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VF531_08200"
     CDS             <1..879
                     /locus_tag="VF531_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX7713994.1"
                     /translation="IFPPLIFLGVGAMTDFGPLIAMPSSLLLGAGAQFGVYFAFIVAL
                     LLGFTPQEASSIGIIGGADGPTSIFLASKLAPHLLGPIAVAAYSYMALVPLIQPPIMK
                     LMTTKEQRAVKMDQLREVSKAEKIVLPLIVAGLVSLLLPSIAPLIGMLMLGNLFRESG
                     VVERLSDTAQNSLINIVTIFLGLTVGATASAEIFLRVDTLKLIVLGLVAFAFSTFGGL
                     LIGKIMYKVTGGKVNPLIGSAGVSAVPMAARVSQVVGQKENPSNFLLMHAMGPNVAGV
                     IGTAVAAGVLLTLFGH"
     gene            1099..1938
                     /locus_tag="VF531_08205"
     CDS             1099..1938
                     /locus_tag="VF531_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713995.1"
                     /translation="MGAGLGNLQQWLIKNRLPLWWGVFLPCMMVLLFFGRELFRIPAP
                     ILENTWISFQSKVLNTIPLIILARIVVAAAGIVALMICFLFPILRVSKEGVQWTKEFG
                     EELANVSGEITGEEIAHLVVKETLRWSLIYGWLRLREQGESDPHLLLREFLGTIWETF
                     PECRLSLTWIDGDAQWTVLHPLLSRLVREEAVSLVSDEHSFGVRLELEGGNGLVLRIY
                     ANDAAGFSQVDERFLTVLGEAFLQEVVRGGFSGAELLTYFQKTSFDFIPHETPKDTGG
                     EPG"
     gene            1935..2168
                     /locus_tag="VF531_08210"
     CDS             1935..2168
                     /locus_tag="VF531_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7713996.1"
                     /translation="MSENGAASDAGSDPSKNESKNECTGRERCKATPLERIDGANRNG
                     SSHKQDPYVARAAERARNKLRDRIFQLNGGRVN"
     gene            complement(2220..3080)
                     /locus_tag="VF531_08215"
     CDS             complement(2220..3080)
                     /locus_tag="VF531_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7713997.1"
                     /translation="MKLKQFWHQNLQWILFIWCISSLILRPGWVIFFAGVLGWVAIVY
                     LTAPGVFWTYFGMLSPSSRFDTAKSARQLQRALDKKPLIPQPYINLGVLYAREKKWAE
                     AIPLFEEALKLATKRDMAETLVILGIAYRENDQPDAALKMLDQAVAAGMKTAKVYLNY
                     ALTYMKQSRLPEALDAAAKARSRNLNATEPVLVMGRIHFALEDYESAKDDYDWAIAHT
                     SWPVESYYWLGRAELELGQTEAAVEHLRKAVERITEDPLLSDITVSEASVWLEKAEKA
                     VPPDSPATAE"
     gene            3319..4095
                     /locus_tag="VF531_08220"
     CDS             3319..4095
                     /locus_tag="VF531_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX7713998.1"
                     /translation="MNESSQSKKTYLIDMDGVLVHGERAIPGASDFIGRLMSGGHKFL
                     ILTNNSRYTPLDLQHRLNTAGINVEAHHLFSSAMATASFVQSQKPGGSAYVLGGTGLY
                     QALNEVGYTLTDYNPDFVILGETESYSYDKIVRATQLIMAGVPFIATNPDTACPTESG
                     TIPACGAVAALLEKASGYHPYFVGKPNPLIMRLALRYLNEHSQNAVMVGDRMDTDIKM
                     GLESGMGTILVLTGVTHRELIGKFPYRPQQVQNSVADIQP"
     gene            4410..5069
                     /locus_tag="VF531_08225"
     CDS             4410..5069
                     /locus_tag="VF531_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7713999.1"
                     /translation="MMNGLIFDFDGTLAETFPVIFPAFRAALLHETGKIYSDEEISAQ
                     FGPSEEGIFQKLLADRWQEGLAIYHAEYRRLHPSLVKLYPGVPELLAWLQNQGLKLAI
                     VTGKGAASLAISVQELGLAGFFDHMESGSPIDGNKVPAITRVVSAWGFDPGTVGYLGD
                     APSDVRCAREAGVIPLGACWAEPGAVKRVTPADRVPCFGSVSEFQQWFRENGTFESGP
                     R"
     gene            5104..6231
                     /locus_tag="VF531_08230"
     CDS             5104..6231
                     /locus_tag="VF531_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7714000.1"
                     /translation="MRICDFKLERYFARYEFQVDCLLSASDCEALTLEELLALADRAG
                     LELWQRLRLGYTESPGHSMLRAEISRIYHKIAPENVLVTAPEEGILLALSAILTTGDQ
                     AIVISPCYQSLSELPAALGAEVVNWPLRLESEWSLDLAELAAKITPRTKLLVVNFPHN
                     PTGFLPTAADWYRLIELARRYNLYLFSDEMYWQLENESDNRLEPAADLYEKGITLSGL
                     SKSYGLPGLRIGWLASRDEAVLRNCARLKDYTTICSSAPSEILAIIALRAKETIIERN
                     RELIRNNTRLAAEFFKSYPSSFRWLPPQGGSVAFPELTASLAVEEFCRDVVERKSLMI
                     LPGTVYDAPGNHFRVGLGRRNLEQGFERLADYIQEFHPEFR"
     gene            6274..7125
                     /locus_tag="VF531_08235"
     CDS             6274..7125
                     /locus_tag="VF531_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX7714001.1"
                     /translation="MRTLWKGAISFGLVNVPVKLYTATEKKEIKFNYLHAKCKTPIKY
                     ERRCPTCNIEVPPEEIVWGYEYQKGQYVVLREEDFERVPDGGTKTIDILDFVDLTEID
                     PIYFEKSYYLEPSPGGEKAYALLRRAMGETGKIAIAKVTIRSKETLAVLRVYQNVLLV
                     ALIFYPDEIRSIAGLEGVRTEPTLHENEVKMANELIANLSGHFEPAKYTNRYREELMA
                     MIQTKISGGEVSEVPERETGKVVDLMEALRASLAATEKQTAGPKKGGGRRKNVAEEPE
                     VITRVRA"
     gene            complement(7155..7352)
                     /locus_tag="VF531_08240"
     CDS             complement(7155..7352)
                     /locus_tag="VF531_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714002.1"
                     /translation="MPKSPGRPEQLHLELKWDYLEWLTVQSEAYTDYETAARKLIDDL
                     PVAGIAEIHPIWRELSGRHLI"
     gene            complement(7742..>8015)
                     /locus_tag="VF531_08245"
     CDS             complement(7742..>8015)
                     /locus_tag="VF531_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7714003.1"
                     /translation="DRTVLHNGVLIYLAVKDRRFSIIGDAGIDQAVPANFWNDVKERM
                     EALFQQGNFPEGVCLGVRMAGEHLATYFPYQSGDRNELPDEISEGK"
BASE COUNT         1854 a         1926 c         2293 g         1942 t
ORIGIN      
        1 atcttcccgc ccctgatctt tctgggggtc ggcgcaatga cggatttcgg cccgctgatt
       61 gccatgccgt ccagtctgct cttgggcgca ggggcgcaat tcggtgtata tttcgccttt
      121 atcgtggcgt tacttttagg gtttacgccg caagaagcct catccatcgg gatcatcggc
      181 ggagcggacg gaccgacctc catctttctg gcgtcaaaac tggcgccgca tctcttgggt
      241 cctatcgcgg tggccgccta ttcctatatg gccttggtgc cgttaatcca gcctccgatc
      301 atgaaactga tgaccaccaa ggaacagcgg gcggttaaaa tggatcagct ccgggaagtc
      361 tcgaaagcgg agaagatcgt cctgcctttg atcgttgccg ggttggtctc actgttgctt
      421 ccctccatcg ccccgctcat cggaatgctg atgctgggaa acctcttccg ggagagcggc
      481 gtggtggaaa ggctttcgga caccgctcag aattccttga tcaatatcgt caccatcttc
      541 ctgggattga ctgtaggtgc gacggccagc gccgagatat tcctgcgcgt cgataccttg
      601 aaactgatcg tcctgggatt ggtggcattt gcctttagca ccttcggcgg gttattgatt
      661 ggcaagatca tgtataaggt caccggcggc aaagtaaatc cgctgattgg ttcggcggga
      721 gtctccgcag taccgatggc ggcaagggtc agccaggtag tggggcaaaa ggagaacccc
      781 tcgaacttcc ttttgatgca tgccatgggg ccgaacgtcg ccggcgttat cggaaccgcc
      841 gtagcagccg gtgtgctctt gacccttttt ggacattaat ccttaaccgg aaagtgaata
      901 cggtagcgtc ttttgttgta atggccacag actcgataac cttgagactg tggcatttta
      961 tttgaattaa tcttgttttc gggatgatct tcgcataagg tcgtggaggt gatgtcgaag
     1021 aattgggaga tccggcggag ataaaaggcg catattccat gcaatccctt catacaatgg
     1081 atcgaatggg gtggagtaat gggtgcgggg ttgggaaacc tgcaacaatg gttgataaaa
     1141 aaccggctgc ccttatggtg gggagtattc ctgccttgca tgatggtttt attatttttc
     1201 ggacgggaac tgttccggat tcccgcgccg attcttgaaa atacgtggat atcctttcag
     1261 tccaaggttc taaataccat tcccttgatc attttggccc gaatcgtggt agcggctgca
     1321 gggatagttg ccctgatgat ctgtttcctt tttcccattc tccgggtcag taaagagggc
     1381 gtgcagtgga cgaaggagtt cggggaggaa ctggccaacg tctcggggga gattaccggg
     1441 gaggaaatcg ctcatttggt cgtaaaggag acattgcgtt ggtcattaat ttacggatgg
     1501 ctgagactgc gggaacaggg agaatccgac ccgcatttac tattaagaga gtttttggga
     1561 accatttggg aaacttttcc cgagtgccgt ttgagtctaa catggatcga tggggatgcc
     1621 caatggacgg ttttacaccc gcttctatcc cgtctggtgc gagaggaagc tgtgtccctc
     1681 gtcagcgatg aacattcctt tggggtaagg cttgagttgg agggtggaaa cggactggta
     1741 cttcggatct atgccaacga tgcggcaggt ttttctcaag tggatgaacg gttcttgacc
     1801 gtcttgggcg aggcctttct gcaggaagtg gtacgaggcg gtttctccgg cgcggaactc
     1861 ttaacctatt ttcagaaaac ttctttcgat tttattcccc atgagacacc gaaagataca
     1921 ggaggtgaac caggatgagt gagaacgggg cggcatcgga cgccggatcg gatccatcaa
     1981 agaatgaaag caaaaatgag tgtaccggca gggagaggtg caaggctact ccccttgaaa
     2041 ggatagatgg cgccaatagg aacgggtctt cccataaaca agatccatat gtagccaggg
     2101 cggcggagcg tgcacgcaat aagctgcggg atcggatctt tcaattaaac ggtggccggg
     2161 taaattgata cccggccggt acgtgcaggt tattcaggca tgggcaaagt gggaaccggt
     2221 tattctgcag tggcgggact atccggtggg acggcttttt cagccttttc caaccagaca
     2281 gaggcttccg aaaccgtgat atcggagagc agcgggtctt cggtgatccg ttccacggct
     2341 ttccgtaggt gttcaaccgc ggcctcggtt tgaccaagtt ccagttcggc ccggcccagc
     2401 caataatagg actcaaccgg ccaggaggta tgggcgatag cccaatcata atcatctttg
     2461 gcggattcat agtcctctag ggcaaaatgg atccgcccca tgaccagaac aggttcagtg
     2521 gcgttcagat tgcgggaacg ggccttagcc gccgcatcca gcgcctccgg gagccggctc
     2581 tgtttcatat aggtcaaggc gtaattcaaa tacaccttgg ccgttttcat gcctgcggca
     2641 accgcctgat ccagcatttt gagggcggca tcgggctgat cattttcgcg gtaggcgatt
     2701 cccaggatta caagggtttc ggccatgtcc cgcttggtcg cgagctttag ggcttcttca
     2761 aacaggggaa tggcttcggc ccatttcttt tcacgggcgt atagcactcc cagattgata
     2821 tagggttggg ggataagagg tttcttatcc aaagcccttt ggagctgccg ggctgacttg
     2881 gcagtgtcaa accgggagct tggggagagc atgccgaaat atgtccaaaa aactccgggt
     2941 gcggttaaat agacaatggc cacccaaccg agtactccgg cgaagaagat gacccatccg
     3001 ggtcggagaa tcaggctgga aatacaccat ataaacagga tccactgcag gttttggtgc
     3061 caaaactgtt tcaacttcat attcaggagc ctccattcat cgaccggtgg ttttattggt
     3121 tcgggatctc gtcacgcctc cggccggatc ataccaacta agattataac aaaaagtctc
     3181 agcctgaaca acttttgccg cggtttattt gcagcgagtc gcggagggat gatataataa
     3241 gcatgaatgg atttcaaaaa tggtccgccg ccatgcggga caatgccgtc tggtgattga
     3301 ccaaggaggt tttttgtgat gaatgaatcg tcgcaatcaa aaaaaacgta tctgattgat
     3361 atggatggag tgctggtcca cggtgaacgg gcaatacccg gagccagcga ctttattggg
     3421 cggttgatga gtggcggcca taaatttctc attttaacga ataactcccg ttacacacca
     3481 ctggatctac aacaccgcct aaatacggcc ggtatcaacg tggaggcaca ccatcttttt
     3541 tcaagcgcca tggccacggc cagttttgtg cagtcgcaaa aacccggcgg ttccgcatat
     3601 gtattgggtg gaaccggact ctatcaggct ttaaatgaag tgggatatac ccttaccgac
     3661 tataatcccg atttcgtgat cttgggtgaa accgaatcat actcctacga taagatcgtt
     3721 cgcgccacgc aactcatcat ggccggagtg cctttcattg ccaccaaccc cgataccgcc
     3781 tgcccgaccg agtccggaac catcccggct tgcggcgcgg ttgccgctct tcttgagaag
     3841 gcttccggat accatcccta ctttgtggga aaacccaatc cattgatcat gcgattggcg
     3901 ctccgctatt tgaatgagca ttcccaaaac gcagtgatgg tcggtgatcg aatggatact
     3961 gacatcaaga tgggactgga gtcgggcatg ggaacgatcc tggttttaac cggagtgacc
     4021 cacagggagc taatcggtaa gtttccttac cgccctcaac aagtacaaaa ttcggtagcg
     4081 gatattcagc cctgattaag gcaacaggca acaggcaaca gcattctatt aggattcgac
     4141 tccgattacc cttaagggaa ccggcaggaa tcttgggcta aagcggttca ggttacgaag
     4201 cataaccaac aaaggccgga gagttatgcg aattccctta aagggatagg gtaatagacg
     4261 cggattcggg cccaaggaaa ccggcgggaa tcttgggcca aaggggttca ggccacgaaa
     4321 catactcatg cctcatgcct gttccctgtt gcctattgcc cttatagact ttcgacttgc
     4381 gacttttgac taactatgaa ggtgcataca tgatgaacgg tttgatcttt gacttcgacg
     4441 gtacacttgc cgagacattt ccggtgattt ttccggcttt tcgggcggcg ctgcttcatg
     4501 agacaggtaa gatttatagt gatgaagaga tttcggcgca attcgggccg agtgaggaag
     4561 ggatctttca gaaactgctt gccgatcgct ggcaagaggg actggcaatc tatcatgccg
     4621 aataccggcg gcttcaccca agcctggtaa aactttatcc cggggtgcct gagttactgg
     4681 cgtggttaca gaatcagggt ttgaaattag ccattgtcac cggtaagggc gccgcaagtc
     4741 tcgcgatctc ggtgcaggag ttgggactgg cggggttctt cgaccacatg gagtccggat
     4801 ctccgataga cgggaataag gtcccggcta ttacgagggt ggtttcggcc tgggggtttg
     4861 acccggggac agtgggttat ctcggggatg cgccttccga tgtccggtgc gcccgggagg
     4921 cgggtgtgat cccgctgggg gcatgttggg ccgaaccggg ggccgtcaaa cgggtcacac
     4981 cggcggatcg ggttccctgt ttcggctcgg tgagcgaatt tcagcagtgg ttccgggaga
     5041 atggaacgtt tgaaagcggc ccgagatagg ggccgtttat ttttatctaa agggggattt
     5101 catttgcgaa tctgtgattt taaattggag cggtattttg cccgctacga gtttcaggtg
     5161 gactgtcttt tgagtgcttc ggattgtgaa gccttaaccc ttgaagagtt actcgccctg
     5221 gccgaccggg ccgggcttga actttggcaa aggctaagat tgggctatac cgagtcaccg
     5281 ggccattcta tgttgcgcgc cgagattagc cgtatatatc ataagattgc tccggaaaat
     5341 gtgttggtga ccgcgccgga agagggaatc ctccttgccc tgagcgcaat tttgaccacg
     5401 ggggatcagg ccatcgtaat cagcccatgt tatcaatctc tatccgaatt acctgcggcc
     5461 ttgggcgcgg aagtcgtcaa ttggccgctg cggttagagt ccgaatggag cctggatttg
     5521 gcggaattgg ccgctaagat tactccccgg acaaaattgc tggtggtgaa ttttcctcat
     5581 aatcccaccg gatttttgcc gactgcggcg gactggtacc gtctgatcga actggcgcgg
     5641 cgctacaatc tttatttatt ctcggatgag atgtattggc aattggaaaa cgaatctgat
     5701 aacaggttgg aacctgcggc ggatctctac gaaaagggga taaccctgtc gggtctttcc
     5761 aagagttacg ggttgccggg attgcgtatc ggctggctcg ccagccggga tgaggcggta
     5821 ctccgtaact gtgcacggtt gaaggattac accacgatct gcagcagcgc gccgagtgag
     5881 atcttggcca tcattgccct gcgggcgaag gagacaatta ttgagcggaa ccgagagttg
     5941 atccgcaata ataccaggct cgcggcggag ttttttaaga gttacccttc gagcttccgc
     6001 tggctgcctc cgcagggcgg ttcggtggct ttcccggagt tgaccgcttc gcttgcggtg
     6061 gaagagttct gccgggatgt ggtggaaagg aagagtctga tgatcctgcc gggaacggtc
     6121 tacgatgcac cgggaaacca tttccgggtc ggactgggaa ggcgtaatct ggagcagggg
     6181 ttcgagagac tggccgatta tattcaagag tttcatccgg aattcagata gggttcatcg
     6241 atttccggga atactagcct cgaggtgatt gggatgcgta ccttatggaa gggcgccatc
     6301 agcttcgggc tggtcaatgt gccggtcaag ctctataccg caaccgagaa gaaggagatc
     6361 aaattcaatt atctccatgc caaatgcaaa accccgatca aatacgaacg gcgctgcccc
     6421 acctgtaata tagaggtgcc tccggaagag attgtgtggg gatacgaata tcagaaaggg
     6481 caatatgtgg tgctccggga ggaggatttc gaacgggttc ccgatggggg caccaaaacc
     6541 attgatatcc ttgattttgt ggatctgacg gagatcgatc cgatctattt cgaaaaaagc
     6601 tactatctgg agccgagtcc ggggggagaa aaggcatacg ccctcttgag acgggcgatg
     6661 ggtgagaccg gcaagatcgc catcgccaag gtgacgattc gctccaagga gaccctggcg
     6721 gtactccggg tttatcaaaa tgtgctttta gtggcgctca tcttttatcc cgatgagatt
     6781 cgctcaatcg cgggacttga gggagtccgg accgaaccca cgctgcatga aaacgaggta
     6841 aaaatggcga atgaattaat cgccaatctt tccggccatt tcgaaccggc caagtacacc
     6901 aaccgttacc gggaggagtt gatggccatg attcaaacca agatcagcgg cggagaggtg
     6961 agtgaagtac cggagcggga gaccggcaag gtggttgatt tgatggaggc cttacgggct
     7021 agcctcgcgg cgaccgagaa acaaacggcc ggcccgaaga agggcggcgg aaggcggaaa
     7081 aacgtcgccg aagagccgga ggtaataacc cgggtaaggg cttaattaag tacaatcaac
     7141 tataccaacc ggatttaaat tagatgccgg ccggacaatt ctctccaaat cgggtgaatt
     7201 tcggcaatcc cggcaaccgg taaatcgtcg atgagtttgc gggcagcggt ttcataatcg
     7261 gtgtaagctt cgctttgaac ggtcagccac tctagataat cccatttgag ctctaggtga
     7321 agttgttcag gccgtccggg tgatttcggc atccgcgatt cctcgatccg ttagaaattt
     7381 tccttattct tcaaagagtt cgcggtcaat ggcccaaatc ctgcttaaat tttccggtat
     7441 tttacctgat taattgggca attccggctg attttcagtc aaaagtcaat taggcaatag
     7501 gcaacaggca aagggcaata ggggaaaaag caagagtatt atgggtcatt tgtgttgaaa
     7561 gtctatgagc aactcacctc cgacaactat ctcgcgcctt tcccaaaaag gttactctta
     7621 cctaggaggt cgcgctttgt taagcgcgga gcacgaggta ggtatcatgg gtgtacaaag
     7681 gcgactctat acctcatgcc tattgcctgt tccctgttgc cgcgtgcccc gtgggggcgc
     7741 gctacttccc ctccgatatc tcatccggca gttcgtttcg atcaccggac tgatagggga
     7801 aatacgtggc caggtgttca ccggccatcc ggacaccgag gcaaacgccc tccggaaagt
     7861 tgccctgttg aaagagggct tccatccgtt cttttacgtc attccaaaaa tttgcgggca
     7921 ccgcctgatc gatgcctgca tcaccgatga ttgaaaatct gcgatctttg acagctagat
     7981 atatcaagac tccgttatga agaacggtcc ggtcc
//
LOCUS       DASVMB010000286         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_35520,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000286 DASVMB010000000
VERSION     DASVMB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35520"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..89
                     /locus_tag="VF531_08250"
     CDS             <1..89
                     /locus_tag="VF531_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714004.1"
                     /translation="LSLVPPILMFFIFQKYIVEGISTTGLKG"
     gene            97..1677
                     /locus_tag="VF531_08255"
     CDS             97..1677
                     /locus_tag="VF531_08255"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX7714005.1"
                     /translation="MNERADNKMRYNNPIIPGFYPDPSICRVGVDYYLVTSSFEYFPG
                     VPLFHSRDLIHWRQIGHCLTRKSQLPLQGVAPSGGIWAPTIRYHEGIFYMTTTNMNGC
                     GNFYVYTGNPSGEWSEPVWLSMGGIDPSLTFVGNKVYYTTNQSAPDGTPGISQVELDI
                     NTGKMMGDIKFIWGGSGGKAPEAPHLYKIGTYYYLMIAEGGTFFTHMETIARSKSPWG
                     PFESCPGNPILTNMQSNMLDVHCTGHGDLVEDQNGNWWMVHLGIRIAQKYMSHIGRET
                     FLSPVEWDKDGWPIVNHGKSVGLISDGPCLPQIEVNPEVEMDHFEENRLALCWNYLRN
                     PYPEDYSLSYRKSWMTLWGNSYRICDPDSPALIARRQKYFNCVMSTLFEFSPKRENEE
                     AGLVIFITNEFYYKMTKRILDGRMCLVLEKRAEDFFQIAACLEIEDKPLYIKVIADKL
                     KYTFYYGYCAYEMVKFATASARFLACEVVGRCFTGTYVGIYASGNGCKSIAPACFDYF
                     GLGEQSEIGTYQTDCKGS"
     gene            complement(1679..2767)
                     /locus_tag="VF531_08260"
     CDS             complement(1679..2767)
                     /locus_tag="VF531_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HEX7714006.1"
                     /translation="MFQDKEKISGNQALMLTLAGGIGNIFVVLAVPAIQEAGRDGWLS
                     VFIAYGMAIVIGLVLLDLGRRFPNQTIVQVLPMIFGNILGKLAGLAYILGWWLMAPLI
                     IREIAELLRFFLPFTPTLIIIIFMALLVVYAMRKGFEVYARTAELFVVLMILLIVLIL
                     GLNLGNFTWKNLTPVLANGFRPVLKGLWVQFPYALETILFMALWLPCLNKRQDGLRAV
                     LLGIPLAGLLLTVFVAANIAFTGITLTARFIFPVFYMSRYIFIGNFLSGMEAVFMVLW
                     LISSYLEILVFYYPPVVGLAQWLNLKEYRPLILPMMLVTVILTLLPSNVIEVIKLDTL
                     KNPFIILPLGLLIPLAWLVAVIRKLDHS"
     gene            complement(2783..3955)
                     /locus_tag="VF531_08265"
     CDS             complement(2783..3955)
                     /locus_tag="VF531_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7714007.1"
                     /translation="MKQYFFLILILLGMTINVAGCWDQIELDKRAVIMALGIDSAEED
                     GKITLTFQTIIPARLADPLGHSQNDPAVRVISASGTTILEATNNFQKQTNAIPYFLQN
                     RLLIIGEDLAREGIQQVMDYFVRNMQSHPRAWVLIAKGKAANIIKWKSEVNQIPANYI
                     ADLLYSSTRQAITAFATADIHRFILALSSQSTSPATSGIEIVQEQDDEPPEVRIFGTA
                     VFKRDKLAGWMGLRETRGMLWITNQSTQGILETGYPALQKRNLVQQVTRAKGRIKPKL
                     NHGKIEAEVEVKEEGNIGEQDGSLKLTGRSMIRALEKNKAAEMRAEIESCLRQCQQEF
                     HSDIFGFGEEIQRKFPQEWKRLGPRWDTEFSHLQIKVRVRAKIRGTGFITDPINLK"
     gene            complement(3952..>4861)
                     /locus_tag="VF531_08270"
     CDS             complement(3952..>4861)
                     /locus_tag="VF531_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018393321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7714008.1"
                     /translation="KRINIDAVLESGYLEEFIQDAPLSIFPTVGNTETPDKFSAKILE
                     GRVGILVDGTPFALTVPFLFVESFQVAEDYYSRSFFTSLIRLIRFLALHLTIVLPALY
                     VAVITFHPNIVPETLLLTIARTREGIPFPAFVEALLMGIFYEMFREGSVRMPRIVGQA
                     VSIVGALILGEAAVNAGIISPIMVIITGLTAVSGFLLPPQTDSITLLRIPLLVIASFF
                     GLFGIIWSYILIVIHLASLRSFGAPYFSPIMPLTFADLKDSFLRAPWWMMKTRPRVID
                     WQGSAREGEDLFPETPGPKQPGGQES"
BASE COUNT         1362 a         1091 c         1170 g         1238 t
ORIGIN      
        1 ccttgtcact ggttcccccc attttgatgt tctttatatt tcagaagtat attgttgaag
       61 gaataagcac caccggcttg aagggataga gcaaccttga atgaaagagc ggataacaag
      121 atgcggtata acaatcccat catccccgga ttttaccctg atcccagcat ttgcagggtg
      181 ggggttgatt attatctcgt gacaagttct tttgaatatt tccccggtgt gccgcttttt
      241 cacagccgtg acctcatcca ctggaggcag attggccatt gccttacaag aaaatcccag
      301 ttaccgctgc aaggagtcgc gccttcaggg ggtatttggg cgccaaccat cagatatcat
      361 gaaggtatct tttacatgac aactaccaac atgaacggct gcgggaactt ctatgtttat
      421 accggcaatc cttcgggcga gtggtctgaa ccggtatggc tatctatggg agggattgat
      481 ccgtctttga cttttgtcgg aaataaagtt tattatacta caaatcagtc tgccccggac
      541 ggaacaccgg gaatttccca agttgagctg gatatcaata ccggtaaaat gatgggtgat
      601 ataaaattca tatggggtgg ctccggtgga aaggcgcccg aggctcctca cttatataaa
      661 atcggcactt attactatct gatgattgcc gagggaggaa cgttttttac ccatatggag
      721 acaattgcaa gaagcaaatc accatgggga cctttcgaat cttgcccggg aaatccgatt
      781 cttacaaata tgcagtcaaa tatgctggat gtacactgta caggacatgg agacctggtg
      841 gaggatcaga acggcaattg gtggatggtt catttgggta ttcgaatagc tcaaaagtat
      901 atgtctcaca ttggaaggga gacattttta tctcctgtag aatgggataa agacgggtgg
      961 cccattgtta atcatggtaa aagcgttggt ttgatttcag atggtccatg tctaccgcag
     1021 attgaagtta atccggaagt ggaaatggat catttcgaag aaaatcgact tgcgctttgc
     1081 tggaactatc tgagaaatcc atatccggaa gattacagtt tgagttatag aaaaagttgg
     1141 atgacacttt ggggaaattc ctatcgaatt tgtgatccgg attctccggc attgattgcc
     1201 agaagacaaa agtattttaa ctgtgtaatg tctacgttat ttgaattttc ccccaaaaga
     1261 gagaacgagg aagccggatt agtcatattc attacaaatg aattttatta taaaatgaca
     1321 aagcgaattt tggatgggag aatgtgcctt gtcctggaaa agcgagcaga agattttttt
     1381 cagattgccg catgtttaga gattgaagat aagccacttt atattaaagt tatagctgat
     1441 aagttaaaat atacttttta ctatggctat tgtgcatatg aaatggttaa gtttgcaaca
     1501 gcatccgcaa ggtttcttgc atgtgaagtt gtgggaagat gttttactgg aacttatgta
     1561 ggcatttatg cctcgggtaa tggatgtaag tcaattgccc cggcgtgttt cgactatttc
     1621 ggtcttgggg aacaaagtga aattggaact tatcaaactg attgcaaagg atcatagttt
     1681 acgaatgatc cagctttcgg atgactgcga ccagccaggc gagggggatt aacaaaccca
     1741 aaggcaatat gatgaaggga ttttttaagg tatcgagctt aatcacttca atgacattgg
     1801 atggcaacag cgttaagatg actgtgacca acatcatcgg caggattaac ggccggtatt
     1861 ctttcaagtt aagccactgg gccagcccga ccacgggggg atagtaaaaa acaaggatct
     1921 ccaggtaact cgagatcagc cagaggacca taaaaacagc ctccatacct gaaaggaaat
     1981 tgccgataaa gatgtagcgc gacatgtaga agaccggaaa aataaagcgg gcggttaaag
     2041 taattccggt aaaagcaata ttggctgcga caaaaacggt taacaacaac ccggccagcg
     2101 gtatgcccaa taataccgcc cggaggccgt cttggcgctt attcaaacag ggaagccata
     2161 aggccataaa taaaatggtt tccagcgcat aaggaaactg aacccacaag cctttcagca
     2221 ccggtctgaa cccgtttgct aataccgggg ttaaattttt ccatgtaaaa ttccccaggt
     2281 ttagcccaag gatcaaaacg atcaacaaga tcatcagcac cacgaaaagt tcagctgttc
     2341 tggcatatac ttcaaaccct ttgcgcatag cgtaaacaac caataaggcc ataaaaatga
     2401 taatgatcaa ggtaggcgtg aacggaagga aaaagcgaag gagctctgct atttcacgga
     2461 tgatcagggg agccatcagc caccagccca ggatatacgc taacccggcc aacttaccca
     2521 atatgtttcc gaagatcatg ggcaaaacct ggacaatggt ttgattcgga aaacgccttc
     2581 ctaagtcaag taaaacgagg ccgatcacga tggccattcc ataggcaatg aatactgaaa
     2641 gccatccgtc gcggccggcc tcctggatgg ccggaacggc taaaaccacg aaaatattac
     2701 cgatacctcc ggccaaagtc agcatcagtg cttggttgcc ggatatcttt tctttgtcct
     2761 gaaacaaaac atagctcctt tcttatttta agttgatggg atcagtgatg aaaccggtcc
     2821 ctctgatttt cgccctgacc cgcaccttga tttgcagatg cgaaaattcc gtatcccagc
     2881 ggggaccaag ccgtttccac tcttggggaa acttgcgctg gatttcctcg ccgaaaccga
     2941 aaatatccga gtgaaattcc tgctgacatt ggcggagaca actttcaatt tccgctctca
     3001 tctccgcggc cttgtttttt tctaaagccc gaatcatact gcggccggtt aattttagac
     3061 tgccgtcctg ttctccgatg tttccctctt ccttcacctc gacttcggct tcaatctttc
     3121 cgtggttgag ctttggcttt attctgcctt ttgctctggt tacttgctgg actaagttcc
     3181 tcttttggag agcgggataa ccggtttcta atatgccttg ggtagattga ttggtaatcc
     3241 aaagcattcc ccgggtctcc cgcaatccca tccaaccggc tagtttatcc cttttaaaaa
     3301 ccgcggtgcc gaatattctg acctcgggag gctcgtcatc ctgttcctgt acgatttcaa
     3361 tgccggaagt ggccggggaa gttgactggc ttgaaagcgc caggatgaaa cgatggatat
     3421 ccgcggtggc aaaagcggta atggcttgcc tggtgctgga gtacagtaaa tcagcgatat
     3481 aatttgcggg aatttggtta acctcacttt tccattttat aatattggca gcttttcctt
     3541 tggcgatcaa aacccaagcc cggggatgac tttgcatgtt gcggacaaaa taatccataa
     3601 cctgttgaat accttcccgg gccaagtctt cgccgataat caacagccgg ttttggagga
     3661 aataaggaat ggcattcgtc tgcttttgaa aattgttggt ggcctctaaa attgtcgtgc
     3721 cgctggcaga aatgacccgg accgccggat cattttgact gtgtcccaat ggatcggcca
     3781 agcgggccgg gatgatcgtt tgaaaggtca aggtaatttt tccatcctct tcggcgctat
     3841 cgattccaag cgccatgatg acggcccgtt tgtccagttc aatctgatcc caacatccgg
     3901 caacattgat ggtcatcccc aaaaggatca gtattaaaaa aaagtattgt ttcatgactc
     3961 ttggcccccg ggctgtttcg gtccgggcgt ttcggggaag aggtcctccc cctcccgggc
     4021 tgatccttgc cagtcgataa cacgcggcct ggtcttcatc atccaccagg gcgccctgag
     4081 gaaagaatcc tttaagtctg caaatgtaag cggcataatc ggcgaaaagt aaggagcgcc
     4141 gaacgaacgt aatgaagcca agtggatcac aatgaggata taactccaga taattccgaa
     4201 aagtccgaag aaggaagcga tcacgagcag cggaatccgg agtagggtga tggaatcggt
     4261 ttgcgggggg agtaaaaacc cggacaccgc agttaatccg gtgataatga ccataatcgg
     4321 gctaatgatt ccggcattaa ccgccgcttc gcccaggatc agcgctccga caatactgac
     4381 cgcctgtccg acaattcgcg gcatccggac cgacccttcg cggaacattt cataaaaaat
     4441 ccccatcaac aatgcctcaa caaaggcagg aaagggaatt ccttcccggg ttctggcgat
     4501 ggtcagcaat aaagtttccg gaacaatatt gggatgaaag gtgataaccg cgacatagag
     4561 cgcaggcagg acaatcgtta gatggagcgc cagaaaacgg atcaagcgga ttaatgaagt
     4621 aaaaaaagac cgcgagtagt aatcttccgc cacctgaaag ctttcaacaa acagaaacgg
     4681 gacggtcagg gcaaatggcg tcccgtcaac caaaatgccg acgcgaccct ccaaaatttt
     4741 ggcgctaaat ttatcaggag tttccgtatt gccgactgtc gggaagatgg aaaggggagc
     4801 atcctgaatg aattcttcaa gataacccga ttccagtacc gcgtcgatat taattctttt
     4861 g
//
LOCUS       DASVMB010000287         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_328774,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000287 DASVMB010000000
VERSION     DASVMB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328774"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..634
                     /locus_tag="VF531_08275"
     CDS             53..634
                     /locus_tag="VF531_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7714009.1"
                     /translation="MASLLYIKANPKSDRDSRTFQISERFVAAYKASHPGDEIITLNL
                     YRENIRPLTGEEVTLTHQSGKEDYPMIRYARQFHNADKYVIAAPMWNLSIPSILKAYI
                     DCIMIVGINFRYTEQGPQGKAVHITSRGGNYSEGPYAAYEMGDRYLRTVFGFLGITDF
                     GTIAAESLDVQGMDVKAIVDRTIKDAEARAKPF"
     gene            complement(1071..1157)
                     /locus_tag="VF531_08280"
     tRNA            complement(1071..1157)
                     /locus_tag="VF531_08280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1121..1123),aa:Leu,seq:cag)
     gene            1483..2679
                     /locus_tag="VF531_08285"
     CDS             1483..2679
                     /locus_tag="VF531_08285"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7714010.1"
                     /translation="MFISEKVNTALSHSSWIRRMFEEGDRLRQIHGAANVYDFTLGNP
                     DLEPPESFSERLRELANHPLPGMHKYMSNAGYPETRQAVADYWSRKTGLPFTAGHVVM
                     TVGAGGALNVVFKTILDPGDEVITSAPYFVEYGSYVDNQQGRLIAVPSRSDFQLDVEA
                     ISRAISPRTKAVLINSPNNPTGIIYSREILQNLAQVIREKESEYGSVIYLVSDEPYAG
                     LVYDQTPLPLVFTIFDHCLLVTSHSKDLALPGERIGYVAVSPRISQAQLVFDGLVFAN
                     RVLGFVNAPSLMQRLVADLQGQVVGVEIYRERRDLLYEHLVRIGFEVVKPQGAFYLFP
                     RSPIPDDVAFIQAAVKYNILLVPGSGFGRAGYFRIAYCVPKELIINSLPAFTRLAQEF
                     GLKPQV"
     gene            complement(2749..>3242)
                     /locus_tag="VF531_08290"
     CDS             complement(2749..>3242)
                     /locus_tag="VF531_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7714011.1"
                     /translation="RTRNGRIGVLATNVTLNGSRFSVLVEKYGTGVEVYTSPAPGLVS
                     LVEAGKLDTAETWELLHKYLDPLMEKGIDTLVLGCTHYPFLRPVIEKICGPEVAVLDT
                     GPAVARQARRILEQRNLLNPENGPGKETFYTSGDPAAVAEVIAKLWPDPVSRVLPVRM
                     NQA"
BASE COUNT          779 a          857 c          805 g          801 t
ORIGIN      
        1 aaataatagt catcaagcag aaggcttcat atcacgagag taaggtgaaa aaatggcttc
       61 tctcctctat atcaaggcca atcccaagag cgatcgcgat tcccgaacct ttcaaatctc
      121 ggagcgcttt gttgccgcgt ataaagcaag ccacccgggg gatgagatca ttacgctcaa
      181 tttataccgg gaaaacattc gccctttaac cggggaggaa gtaaccctca cccaccagtc
      241 aggtaaagag gattatccga tgatccgtta tgcgcggcaa ttccataatg ccgacaagta
      301 tgtgatagcc gcccccatgt ggaacttgag cattccttcg attttaaaag cttatatcga
      361 ctgcatcatg atcgtaggaa tcaacttccg ttacacggaa cagggtcctc agggtaaagc
      421 agtccacatt acctcccgcg gcggtaatta ttcggaagga ccctacgcag cttacgaaat
      481 gggggatcgc tacctgcgga cggtctttgg ttttttggga atcaccgatt tcgggactat
      541 cgctgccgag agtttggatg ttcaggggat ggatgtaaag gccatcgtag atcggaccat
      601 caaggacgcg gaagcccgcg ccaaaccctt ctaaaagcca cttttattca gagtggcttt
      661 tatcatatga ctacccattt aggtagggga ttaaggtctg gtcgaggccc tcacccctca
      721 gccaacatcc gggtcggccc tcccccataa cgctatcaaa aagcgatagc tatggaagag
      781 gggccgaaga tgcctaaatt catgagagtt aaggatttag gcctatttct ccctctccca
      841 tagcgagtgt ttgatcttcc ctaagtttct tcgagcgcga tgagagaggg ccgctgactt
      901 gaatcagccc gatgggtgag ggcattaaga gcggagccta cagtcgtttt ctgagtaaac
      961 tggatactca tatacataaa tgaaggcaac tatgcctcca cagtcatttt tagaggcgat
     1021 aaggttcaga aaataaaaaa ccttcgtcgt attgacgaag gataatttct tggtgccgaa
     1081 ggcgggattt gaacccgcac gcccttgcgg acacagcgcc ctgaacgctg cgcgtctgcc
     1141 agttccacca cttcggcacg tcataacgta taataaatta taaacgattt atgcgaagct
     1201 gtaaagagga accctgataa aaatttctgg aaaagccccc tgatttatct atgcaatgtt
     1261 ttcgtgtgtt gggtgtactt cgttatgaaa agtcagttac ccttcgtcta attattcccc
     1321 accaagctgt tcaaactcga ttatcggcaa atgaattttc aactgtccca aaaagccggc
     1381 aggattaacc ggaaacggca tggaataata ttgaaatact tttgcgggat cgggtcctta
     1441 tccggcgacg ctccttcccg cctgaaagtg aggtcgtttt ctatgttcat ctcggaaaag
     1501 gtcaataccg cccttagcca ttcctcctgg atccggcgga tgtttgagga gggcgatcgg
     1561 ctccgtcaga ttcacggggc agccaatgtt tacgatttta cattgggtaa tccggatctc
     1621 gaaccaccag aaagctttag cgaacgcctc cgggaactcg ccaatcaccc attgccgggg
     1681 atgcataagt atatgagtaa cgccggatat cccgaaaccc ggcaagcggt ggccgattat
     1741 tggagccgca agaccggcct cccctttacc gcaggccatg tggtaatgac ggtcggagcc
     1801 ggcggcgcgc tcaacgtggt attcaagaca attctggatc cgggggatga ggtaattacc
     1861 agtgccccct attttgtgga gtacggttcc tatgtcgaca atcaacaagg gaggctgatt
     1921 gccgtaccgt cgcgttccga cttccagttg gatgtggaag ccatcagccg ggcaatctct
     1981 ccccggacca aagccgttct cattaactcg cccaataacc ccaccggtat aatttacagt
     2041 cgggagatcc ttcaaaactt ggcccaagtg atccgggaaa aagaatccga gtacggttcg
     2101 gtaatctact tggtttccga tgaaccctac gccgggttgg tttatgacca gaccccgtta
     2161 ccgctggtct tcacgatctt cgatcattgt ttactggtaa cctcccatag caaggacctg
     2221 gctttacccg gcgaacgcat cggctatgtg gccgtaagtc cccggatctc ccaagcgcaa
     2281 ttggtctttg acggtttggt ttttgccaac cgggtcttgg gatttgtcaa cgctccttcc
     2341 ctgatgcaac ggttggttgc ggatctccag ggacaggtgg tcggagtgga gatttaccgg
     2401 gaacggcgcg atctccttta tgaacatttg gtccggatcg gttttgaggt cgtcaaaccc
     2461 cagggcgcat tctacctttt ccccaggagc cccatcccgg atgatgtcgc ctttattcaa
     2521 gcagcggtaa aatataatat tctcctggtg cccggtagcg gctttggacg agcgggatat
     2581 ttccggattg cctattgtgt gcccaaagaa ctgatcatca attcgcttcc ggcctttacc
     2641 cgtctggcgc aagaattcgg cctaaaaccc caggtctaaa tacatccata tatgtgcgta
     2701 gggaccaggc attcctggtc cctacgcatg cctggcccgt ttatattatc acgcctgatt
     2761 catccgaacc ggtaacaccc ggctcaccgg atccggccat aatttggcga tgacctccgc
     2821 cacggcggcg ggatcaccgc tggtgtaaaa ggtctctttc cctggtccgt tttcgggatt
     2881 gagcaggttt ctctgttcga gtattcgccg agcttggcgg gccaccgccg gacccgtgtc
     2941 gagaactgca acttccgggc cacaaatctt ctcgattacc ggtcgcagga agggataatg
     3001 agtacatccg agaaccagcg tatcgatacc tttttccatt aagggatcaa ggtatttgtg
     3061 gagtaactcc catgtctctg cggtatccag tttgcccgct tcaaccagtg aaaccaaccc
     3121 cggtgccggg ctggtgtaca cttccacccc ggtgccgtat ttctccacca acaccgaaaa
     3181 acgcgaacca ttcaaggtca cattggtggc caatacgccg atcctgccgt tgcgggtacg
     3241 tt
//
LOCUS       DASVMB010000288         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_180492,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000288 DASVMB010000000
VERSION     DASVMB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180492"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VF531_08295"
     CDS             complement(<1..827)
                     /locus_tag="VF531_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7714012.1"
                     /translation="MENHPAFPEESARLRETLKQVNQSLEDATQRKQYTDKELQRLER
                     VGNADNSQGYIDLMVGQALKEGLVLKLRNLEGAQKKPYFARIDFTEAGKKKPEKLYIG
                     KLSLTREEDQQPIIIDWRAPISNLYYESRLGPASYHCPEGEIGGDLQLKRQFSIEEGI
                     LERIFDIDITTNDQFLQSSLGANAADRLKDIVATIQAEQNRIIRADLEQPLIVQGAAG
                     SGKTTIALHRIAYLIYTYNRSFRPEQFMIIAPNRLFLNYISEVLPELGVERVKQSTF"
     gene            complement(1183..1899)
                     /locus_tag="VF531_08300"
     CDS             complement(1183..1899)
                     /locus_tag="VF531_08300"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEX7714013.1"
                     /translation="MSKGRILFGVVLILIGLLAILNSFQITDISFGRLVADYWPVLLI
                     LWGMEVLLEQNGSNAKIIGSIILLVGVAFLGRNLHWFYFDAAIFWRLFWPGILVLLGI
                     RLLLGSSFDARTNLAFMGGIERKDNWQVKNNSYWAFMGGVDLDLREAIIPDQVITFTV
                     TALMGGVDIIVPPDLPVVCEGTSILGGVELLGKSNGGILANLRAEQGDIKGPKLLRIN
                     SLAIMGGVEVKAVPRGAAIP"
     gene            complement(1987..>2987)
                     /locus_tag="VF531_08305"
     CDS             complement(1987..>2987)
                     /locus_tag="VF531_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714014.1"
                     /translation="HFQAGGSSRRSVATNVSFKAQSTTAFQQSNRVRASLSVTIRQQI
                     LVLERVLTGLTGQRIPIYWIADTYTAGTERMTERLRYRVIQAFMDRQHESESQRSSEI
                     WEDEQLNFNAQGYFQTDDGKLVQFDCTLHLGREFASRNGRLLENRDKLLDPLVINFNG
                     TAADLTQTHFHFDLDCDGAAEELPFVTEGSGFLALDRNQDGKVNDGSELFGPTVGNGF
                     EELAAFDQDRNGWIDENDPIFERLRIWTRDASGQEELVALGQKGVGAIFLGNVAAPFD
                     MRDVQNSNLAVNRSAGIFAREDGTVGTIQQLDLVIPADMQPSKTAFGRKKDKLVES"
BASE COUNT          718 a          832 c          681 g          756 t
ORIGIN      
        1 tcgaaggtgg attgtttgac ccgttctact cccagttccg gcagaacttc ggagatataa
       61 ttcaggaaga ggcggttcgg ggcgatgatc ataaactgct ccggccggaa ggaacgattg
      121 taagtataga tcaaatatgc gatccggtgc agagcgatag tggttttgcc gctgcccgcg
      181 gcaccttgca cgatcagggg ttgctccaga tcggcccgga taatccggtt ctgctcggct
      241 tgaatggtgg ccacgatatc ctttaggcgg tcggcggcat tggccccgag cgaactttga
      301 aggaactgat cgttcgtcgt gatatcgata tcgaagattc gttccaatat cccctcttcg
      361 atggagaact gccgcttgag ctgaaggtcg cccccgatct ccccctccgg gcaatggtaa
      421 ctcgccggcc ctaaccggct ttcataatac aggttggaga tgggcgcccg ccagtcaatg
      481 atgatcggtt gttgatcctc ttcccgggtc aaactcaact taccgatata gagtttctcc
      541 ggtttcttct tgccagcctc ggtgaaatca atccgggcga aatagggttt tttctgtgcc
      601 ccttccaggt tacgcagttt taagaccaat ccttctttta gggcctgacc gaccatgagg
      661 tcgatatatc cctggctgtt gtcggcattg ccgacccgct ccaagcgttg caactccttg
      721 tctgtatatt gcttgcgctg tgtcgcgtct tcaaggcttt ggttaacctg tttgagagtc
      781 tcccgaagcc gggcagattc ttcgggaaaa gcgggatgat tctccactgt cgatatactc
      841 ctttgtttta ttcaaagcat cgggagtttt attttactat aaaacaatgt taatacaaac
      901 cactgtaaaa agctccagct ctttttgccg tcacttctgt ccaaatcaac gggcgttttc
      961 catttttctc agatttttat gaaattacac ttgacaagtc gatcatttca tcctataata
     1021 taataacaaa agataaagaa cctgttaatc gtggagatta tcaggttctt tccattttat
     1081 ggaaaacaat ccttccaaat gggatttctc agctaatcaa aaagcgggtg atacgctcaa
     1141 cccgcttttt acttgtctta ggttaccgct ctccgccttt tactagggga tcgctgctcc
     1201 ccgcggaacc gccttgacct ccaccccgcc cataatggcc agggagttga ttcgtaataa
     1261 tttcggaccc tttatatccc cctgttcggc ccggagattg gccaggattc ccccattact
     1321 ctttcccagc aactcaacgc ctcccaggat ggaggtccct tcgcaaacca ccggcaaatc
     1381 aggcggaaca ataatgtcca ccccgcccat aagggcagtc actgtaaagg ttatgacctg
     1441 atccggaata atggcttcac gaagatccag atccactcca cccatgaaag cccaataact
     1501 gttgttcttt acctgccagt tatccttgcg ctcaatcccg cccatgaaag ccagattggt
     1561 ccgggcatcg aaactcgaac ccagcaacag tcttatgccc agcaggacca agatgcccgg
     1621 ccagaacagc cgccaaaata ttgcagcatc aaagtaaaac caatgcagat tacgtccgag
     1681 aaatgccaca ccgaccagga ggatgattga accgatgatc ttggcgttcg aaccattttg
     1741 ctccaggaga acttccattc cccataagat cagcaaaacc ggccagtagt ccgcgaccag
     1801 ccttccgaag ctgatatccg taatctgaaa actgttgagt atcgccagca agccgataag
     1861 aatcaaaacc acaccaaaaa gaattcgtcc tttggacaaa gccatcactc cttcgcgcca
     1921 gaactggttg acgcaaccaa gcctttacag tacctttttc ctataattta tgataaacct
     1981 ttatcctcat gattccacaa gcttatcttt tttcctgccg aaagccgttt tcgatggttg
     2041 catatcggcc gggatcacca aatccagctg ctggatcgta ccaacagtcc catcctcccg
     2101 ggcaaagatt cccgcgcttc gattcaccgc caggttggaa ttttgaacgt cccgcatatc
     2161 gaaaggtgcc gctacatttc ccagaaagat cgcacccact cccttttggc caagggcaac
     2221 gagttcttct tgacccgaag cgtcccgggt ccagattcgc aaccgttcaa aaatcggatc
     2281 attctcgtcg atccagccgt tacggtcttg atcaaatgcg gccagttcct caaaaccgtt
     2341 accgaccgta ggcccgaaga gttcagaacc gtcgttcacc tttccgtcct ggttccggtc
     2401 aagcgccaaa aagccactac cctcggtgac gaaggggagt tcttccgccg ctccgtcaca
     2461 atccagatcg aagtggaaat gagtttgggt cagatctgca gccgtgccgt taaagttgat
     2521 aaccagcgga tcaaggagtt tgtcgcggtt ttcgagcagc cggccgttac ggctcgcgaa
     2581 ctcacgtcca agatgcaggg tgcaatcgaa ctgtaccagt ttcccgtcat ccgtttgaaa
     2641 atacccctga gcattgaaat ttagttgttc atcctcccaa atctccgacg aacgctgcga
     2701 ctccgattca tgctgccggt ccatgaacgc ctgaatgacg cgataacgta gtcgttcggt
     2761 cattctttcc gtccctgcag tgtaggtatc cgcgatccag taaatcggta tccgttgccc
     2821 tgtcaatccg gttagcaccc gctccaatac taaaatctgt tgccggatgg ttactgaaag
     2881 gctggcccgg acccggttgg attgctgaaa ggcggtcgta gactgggctt tgaaggaaac
     2941 attggttgcg acggagcggc gggaactccc gcccgcttga aaatgca
//
LOCUS       DASVMB010000289         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_36245,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000289 DASVMB010000000
VERSION     DASVMB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36245"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VF531_08310"
     CDS             complement(<1..683)
                     /locus_tag="VF531_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7714015.1"
                     /translation="MSTNPVVRPSCPGGTLYAIRPGDSLFSLARRFNTSVMAILEANP
                     GINPANLQLGETLCIPIALTSILPTTGGCPGGFLHTVVTGDTFFSIAQNAGIAVAALA
                     AANPGVDPNRLQIGQQICVPAPAPPAVPCPGTLYTVQPGDTFIGLARRFGYTLDAILA
                     ANPGVDPRSLQIGQRICLPPAPGAGPFPCYGGSIYRVQPGDTFYGIARKFGLTFQTLR
                     ASNPDITNP"
     gene            complement(1022..2221)
                     /locus_tag="VF531_08315"
     CDS             complement(1022..2221)
                     /locus_tag="VF531_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7714016.1"
                     /translation="MNRNWTLSGTGLGLFLIALGIGYFTDLATGSTWLYQIFRFYPVL
                     IILLGLDHLLGGYYGSSAKIHPPQSWIITVIILITIGGLTASIVPRIFRENMDFRSFN
                     WHYHWIGNNFKSEREIEKRYPLPPSVKTLKIENNSGTIWIEPSTSGTVTVNAVIRVRA
                     SNSGELKSYADAVDLSGEKTGDQLRIKLVSKQTGADPDPAHFSSDLQVNVPAGVSVTV
                     INSLGDVRVGSIAADLRIEVASGTIHVERAGGNLDALSDLGSIEVGTVGGDAHLTASA
                     GEIRADLIQGTATVESSLGGVKLDACEGPVKARLRSGSLTVNLRKITGPSDLEVDLGS
                     AVIGLPQDARCAIDAATSMGDIHSDFPLAIRREVAQASANGELNGGGPLVRIKADSGS
                     IQLRNAF"
     gene            complement(2223..2747)
                     /locus_tag="VF531_08320"
     CDS             complement(2223..2747)
                     /locus_tag="VF531_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714017.1"
                     /translation="MSEIRKKSPVAGGILSFIFPGAGQLYNEDYSKGIVLIVAAIATI
                     VTIVYHALSLGGIVMSGEIYSGTMQIVTIVAAGLIYLGIWIYGIIDAIITAQHISAAV
                     NSGSITAETRKVRSQEGMIGLGVVLIIIGVLGVLIQMGFKLEYLIRYGLPIALILIGA
                     YLLAKTNGWIKGGK"
     gene            complement(2925..3350)
                     /locus_tag="VF531_08325"
     CDS             complement(2925..3350)
                     /locus_tag="VF531_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714018.1"
                     /translation="MRRNLSPVSRRAPAREYAAGSGLMEQLLADAFNDDTLCDLPEYP
                     SDADFLRDLQRRLSQQQSVLSYDQVWFSGILRGMAATAVGVVLFVGLNTLPPIFINAS
                     ITGSGMLREKLVSISAGQHVLVQRLITRIQSQNPNAGRN"
     gene            complement(3325..3918)
                     /locus_tag="VF531_08330"
     CDS             complement(3325..3918)
                     /locus_tag="VF531_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7714019.1"
                     /translation="MGSSEIESELELIEQAKNGNQQAFGKLALRYRKNVFGLAYRMTG
                     HPEEAEDLTQDVFLRAYLSLHRFTPNRDGAFKSWLLTITSRLCIDRARHKRSHPGESL
                     EDYEQELPTGEKSVLETVTEEETREQVRMALRLLPENYRMAMILKYQEDLDYQAIARI
                     MKIPLGTVATLLNRGRAQLKKILLERGIIDEKELVAR"
     gene            complement(4066..>4202)
                     /locus_tag="VF531_08335"
     CDS             complement(4066..>4202)
                     /locus_tag="VF531_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714020.1"
                     /translation="GEQGNVLKYWFTVLYPGLAIILFVLSWNLIGDALRDIMDPKIQA
                     "
BASE COUNT         1095 a         1052 c         1083 g          972 t
ORIGIN      
        1 tccgggttcg tgatatccgg attactggca cgcaaggttt ggaaggtgag accgaatttc
       61 ctggcaatcc cgtaaaaagt gtccccgggt tgaacacgat aaatggaacc accgtagcag
      121 gggaaagggc cggctccggg ggcggggggc agacagatcc gttgaccgat ttggagactc
      181 cggggatcta ccccgggatt ggccgccaat atggcatcca aggtataacc aaaacgccgt
      241 gctaaaccga tgaaagtgtc ccccggttgg acggtataaa gcgttccggg acaaggcact
      301 gccggagggg cgggggctgg aacacagatc tgctgtccga tctggagtcg gttcggatcg
      361 actccgggat tggctgcggc taaggctgct acagcgattc cggcgttctg tgcaatactg
      421 aaaaaggtat caccggtgac cacggtatgg agaaaacctc ccggacaacc gccagtggta
      481 ggcaaaatgg aagtgagtgc aatcggaatg cataaagtct ctcctaattg tagattcgcc
      541 ggattgatac cgggattggc ctctaggatc gccatgaccg aggtgttaaa acggcgggcc
      601 aaactgaaaa gcgaatcccc gggtcgaatt gcgtataagg taccaccggg acatgaagga
      661 cggaccacag gattggtgct catggcatac agctccttaa aataggcaac aggcaacagg
      721 caactggcaa taggtaataa gtgtaaattt caagggaaaa agtgtagggc gggggtgagt
      781 tgctaatagt taaggcatag gacttctgct attggtatga cggattttaa acaagaataa
      841 ataacttgca atactattgc ctattgcctg ttgccgattg cctactgcct ttattgactt
      901 cgtctcctcc gatagcatat gctaatagac tccgagcggc aatcggaacg caaactggga
      961 aataaaaaaa tacccgggtt aagggtattt tttctctata gatcaactat ttatatcgct
     1021 gttagaaagc atttcgcaat tggatactac cgctgtctgc tttgatccgg acgaggggtc
     1081 cgccgccatt caattctccg ttggctgagg cttgggctac ttctctgcgg atggccagcg
     1141 ggaaatcgga atgaatatct cccatggagg tggctgcatc gatagcgcat cgggcatcct
     1201 gaggcagacc aatgacggcg ctccccaaat ccacttccag atcactggga ccggtgatct
     1261 ttcgaagatt aaccgtcagg ctgccgcttc tcaatcgggc tttgacagga ccctcgcagg
     1321 catcaagctt gactccgccc aaggaactct ctacggttgc ggtaccttga atcaggtcgg
     1381 cgcggatttc tccagcagaa gcagtcaggt gcgcatcacc accgacggtt cccacctcaa
     1441 tcgaaccaag atcgctgagg gcgtccaaat ttcctcccgc ccgttccaca tgaatcgttc
     1501 cggatgccac ttcgatacga agatcggcgg cgatggaacc gacccgaaca tcgccgaggg
     1561 agttgatcac agtcactgag accccggcgg ggacgttgac ttgaaggtcg gacgagaaat
     1621 gggcgggatc cggatcagcg cctgtctgtt tcgacacgag tttgatccgc aattgatccc
     1681 cggttttttc cccggataaa tcgacagcgt cggcatagga tttgagttca ccggaattgg
     1741 aggcacgaac ccggatgacg gcatttaccg taactgttcc ggaggtgctc ggttctatcc
     1801 agattgttcc ggaattgttc tcgattttta aggttttgac acttggtggg agaggataac
     1861 gtttctcaat ctcccgctcc gatttaaagt tattaccgat ccaatgataa tgccaattga
     1921 agcttcgaaa gtccatattt tcccggaata tcctgggaac gatgctggct gtaagaccgc
     1981 cgatggtaat cagaatgatc acggttatta tccaactctg aggcgggtgg atcttggcgg
     2041 atgaaccata gtaacctccc agcagatgat ccagacccaa gaggataatc aacacgggat
     2101 aaaaacgaaa tatctgatat aaccaggttg aacccgtagc cagatcggtg aagtagccga
     2161 tacccaaggc gatcaggaat agccccagcc cggtaccgga aagggtccaa ttacggttca
     2221 tgctactttc cccccttgat ccagccgttc gttttggcca agaggtaggc gccgattaaa
     2281 atcaaagcta tcggcaggcc gtaccggatc aggtactcaa gtttgaaacc catctgaatc
     2341 aggactccca agactccaat aatgattagc acaaccccca acccgatcat accttcctgg
     2401 gatcgaacct tacgggtctc ggcggtgatt gatccggaat tgacggccgc cgagatatgc
     2461 tgggcggtaa tgatcgcgtc aatgataccg tatatccaaa tccccagata aatcaaaccg
     2521 gctgcgacaa tcgtgacgat ctgcatcgta ccggaataaa tctcaccgct catcacaatg
     2581 ccgcctaaag ataaagcgtg ataaacgatg gtgacgattg tggcgatggc cgccacgatc
     2641 aagacgatcc ctttgctgta gtcctcgttg tagagctgac cggctccggg aaaaataaac
     2701 gagagtattc ctcctgccac tgggctcttt ttgcgaattt ccgacatacg taatccccct
     2761 ttaaagttta aagtgaaact tctgaggcaa caggaaacag gcaacaggaa acaggcaata
     2821 ggcaacagca agcacacagt caagactttg ttgaacgttg tttttataag tatcagctaa
     2881 cgacggttgc tgttccctgt tgccacatta atgcctgcct aatcctaatt ccggcctgca
     2941 ttcgggtttt gactctgaat ccgggtaatt aacctttgca ccaagacgtg ctgcccagcg
     3001 gagatactga ctaatttttc ccggagcatt ccgctccccg taatcgaggc gttaataaat
     3061 atagggggga gggtattgag acctacgaat aggacaactc cgacagcagt ggcagccata
     3121 ccccgcagaa ttccgctgaa ccagacctga tcataggaca gaacggattg ctgttgtgaa
     3181 agccgccgct gcaaatctct taaaaaatcg gcatcggaag gatattcggg aagatcacat
     3241 aaggtatcat cgttgaatgc atctgcaagc aactgttcca ttaatccgga gccggcggca
     3301 tattcacgtg cgggggcgcg ccgactaacg ggcgacaagt tccttctcat cgataatccc
     3361 cctttcgagt aagatctttt ttaactgagc ccggccccga tttaataatg tggcaaccgt
     3421 acctaaaggg atcttcatga tccgggcgat ggcttgataa tccaaatctt cttgatactt
     3481 gaggatcatg gccatgcgat aattctcggg aaggagtctt aaggccatgc ggacctgttc
     3541 ccgtgtttcc tcctcggtta cggtttccag gacacttttt tcgcccgtcg gtaactcttg
     3601 ttcgtagtct tccaggcttt caccgggatg gctgcgtttg tggcgggcac gatcaataca
     3661 gagccgggaa gtgatggtca ggagccatga tttaaaagcg ccatcccggt tgggagtaaa
     3721 acggtgcagg gaaagataag cccggaggaa aacatcctgg gttaaatctt ccgcctcctc
     3781 gggatggcct gtcatccggt aagccagacc aaaaacattc ttgcggtagc ggagcgccag
     3841 ttttccaaag gcttgctggt ttccattttt ggcctgttcg atcaattcaa gttcggactc
     3901 aatttccgag gaccccaaag aactacctcc atcatcgcgc gtcatgagca gttccgtata
     3961 gtataacggc acttcccgga gttttctttc aagtccgggc gaagagatta aaaaaataaa
     4021 ccgccccggg aacagggcgg tcgagaattg ccacaccaaa ccatattacg cttgaatttt
     4081 gggatccata atatcgcgta aggcatcacc gatcaagttc caggaaagca cgaaaaggat
     4141 aatggccagt cccggataga gtacggtaaa ccaatatttc agaacattac cctgttcacc
     4201 aa
//
LOCUS       DASVMB010000290         5935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_111921,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000290 DASVMB010000000
VERSION     DASVMB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111921"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VF531_08340"
     CDS             <1..1330
                     /locus_tag="VF531_08340"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7714021.1"
                     /translation="RFETASELYRSAQLIWEHTETPGNTASADCIKGLADIDYDAQRY
                     QPALDGYQRAQDINQKILGPDHPEIARLWSDIARCEAALGLNTEAETHIRQAVSSLEK
                     NVDPNSPDLALAYSIRSWVELNLNRADEALKSNEQALTILRKTGGNSAALGWVMYRQG
                     EIDRSCGRYGNAETNFKQAVSILGKYLDRDHPRVAVAFIGWGETLVAEKKYSKALPLY
                     QKAGRIQAKAFGLEDLNVAATAYHQAEVQNILGNYRSAEPLYYQALNIRGRWLGLDGP
                     EVAVCRINLAEIYKVSGRDRDSRTCAETALDTASRQADPENVLTGAVLNLLARFQIEQ
                     GDFDEAQKDLDRATAITKRFYGPDRPEMAKCVATQAKLASARGQYKSAEALFGSSLTV
                     LEKYYGPNHPYVGMILGDQLEWARLSGKIAIAAELSKRLQKIRSQKNGRV"
     gene            1501..1929
                     /locus_tag="VF531_08345"
     CDS             1501..1929
                     /locus_tag="VF531_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX7714022.1"
                     /translation="MTRQNDWMAPVVNPDEFSPAVWAYIGDAVYELFTRSQLLKDGPA
                     KTKQLHKATIARVKASFQAELLKRLEPDLTTDELEVVKKGRNIKSGHIPAYTDMITYR
                     YSTAFEALIGYLYLTGSYQRLQVILAKTLMKESETTGISG"
     gene            1974..2711
                     /gene="rlmB"
                     /locus_tag="VF531_08350"
     CDS             1974..2711
                     /gene="rlmB"
                     /locus_tag="VF531_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7714023.1"
                     /translation="MSEQIEGRNPVIEALRSGHELEKLYILKGGEPNGPLREILGRAR
                     ELKIPIHPVEAQVLDKMAVTRNHQGVIALAAGWKYATVEKIMERAAESGEPPFVLFLD
                     GVADPQNLGSIIRTSEAAGVHGIIIPERRAVGLTAAVCRASAGAVEHMPVARVTNLNR
                     TMEELKAAGLWFTGADMDGAVVYHRADLRGPMGLVIGGEGGGLSRLVKEHCDQTVRLP
                     MWGAINSLNAAVAAGILLYEIRRQRLG"
     gene            3398..4054
                     /gene="sigH"
                     /locus_tag="VF531_08355"
     CDS             3398..4054
                     /gene="sigH"
                     /locus_tag="VF531_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX7714024.1"
                     /translation="MGARPQRETTEIYDEMLDEMIVEAAREGDDAAQEYLINKYKNFV
                     RAKARSYFLIGADREDIIQEGMIGLYKAIRDFRNDKLASFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYVSLNKPIYDEESDRTLLDVISGTKVTDPEELVISREEFVDIENKMG
                     EFLSDLEWKVLMSYLDGRSYQEIAKDLRRHVKSIDNALQRVKRKLERYLEKREEISSL
                     "
     gene            4161..4246
                     /locus_tag="VF531_08360"
     tRNA            4161..4246
                     /locus_tag="VF531_08360"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4195..4197,aa:Tyr,seq:gta)
     gene            4348..4423
                     /locus_tag="VF531_08365"
     tRNA            4348..4423
                     /locus_tag="VF531_08365"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4381..4383,aa:Met,seq:cat)
     gene            4458..4533
                     /locus_tag="VF531_08370"
     tRNA            4458..4533
                     /locus_tag="VF531_08370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4491..4493,aa:Thr,seq:ggt)
     gene            4836..>5935
                     /gene="tuf"
                     /locus_tag="VF531_08375"
     CDS             4836..>5935
                     /gene="tuf"
                     /locus_tag="VF531_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7714025.1"
                     /translation="MAKQKYERTKPHVNIGTIGHVDHGKTTTTAAITLVLSKAGQANF
                     VKFDEIDKAPEEKERGITINTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVWLNKADMVDDPELIELVEMEVRDLLAQ
                     YEFPGDKIPIVRGSGLKALECGCGKRECQWCGRIWDLMDAVDSYIPTPERATDKPFLM
                     PVEDVFTITGRGTVATGRVERGAVKVGDEVQVIGLQPEIKKTIVTGVEMFRKLLDQAV
                     AGDNIGVLLRGIDRKDIERGQVLAKPGSITPHVKFEGEVYVLTKEEGGRHTPFFNGYR
                     PQFYFRTTDVTGVAHLPEGVEMVMPGDNIKMT"
BASE COUNT         1543 a         1330 c         1648 g         1414 t
ORIGIN      
        1 ccgttttgaa accgcttcgg agttgtaccg cagcgcgcag ctgatctggg aacacacgga
       61 gacaccggga aataccgcct cggccgattg cattaaggga ttggcggata tcgactacga
      121 cgctcaacga tatcagccgg cgctggatgg ttatcagcga gcacaagaca tcaatcaaaa
      181 gattctggga ccggaccatc cggagattgc ccggctttgg tcggatatcg cccggtgcga
      241 ggcggcattg ggcttgaata ccgaggccga aactcatatt cgccaggcgg taagttcgct
      301 ggagaagaac gtggatccca atagccccga cttggcattg gcttattcga tccggtcctg
      361 ggtggaattg aatttaaacc gggccgatga ggcccttaaa tccaatgaac aggctctaac
      421 gatcctgagg aagaccggcg ggaacagtgc ggcactgggc tgggtcatgt accggcaggg
      481 tgagattgat agaagctgcg gtaggtacgg taatgccgaa acaaatttta aacaagccgt
      541 atccatcctg ggaaagtatc tggatcgaga ccacccccgg gttgctgtgg ccttcatcgg
      601 atggggcgaa acactggttg cggagaagaa atactcgaag gcattgccgc tttatcaaaa
      661 ggccgggcga atccaggcga aagcttttgg gctagaggat ttaaatgttg ccgcaaccgc
      721 ctatcatcaa gcggaggttc aaaatatcct tgggaattac cgtagcgccg agccccttta
      781 ttaccaggcg ctaaacattc gcggccgatg gctgggtctg gacggcccgg aggtggcggt
      841 gtgccggatc aatctggccg agatctataa agtatctggc agagacaggg attcaaggac
      901 ctgcgcggag acggcattag atacggcatc cagacaggct gatcccgaga acgtgttaac
      961 cggcgccgtt ttgaatctat tggcacggtt tcagatcgag cagggtgatt tcgatgaagc
     1021 gcaaaaagat ttggaccggg ctaccgccat caccaagcga ttttacggcc cggatcgacc
     1081 tgaaatggcc aaatgtgtag caacccaggc caaactagcc tctgcccggg gtcagtataa
     1141 atctgcggaa gcgctctttg gatccagcct gacggttctc gaaaagtatt atggtcccaa
     1201 ccacccttac gttgggatga tcctcggcga ccagctggaa tgggcgcggc tctccggcaa
     1261 aatcgcaatc gccgccgaac ttagtaaacg gctgcaaaaa atccgttccc aaaaaaacgg
     1321 gagagtttga tgggaaaacc ccggataaga tatacaccat gaagacgtat ttggtgaaaa
     1381 cgtccaaatt caggttgcgc ctatgcggaa gatgcgatat acttagaaaa gtttatttgc
     1441 cagaaaaata tttccaggcg gggttttgga gaatacccct cctccataag gtgatgcggt
     1501 atgacgcggc agaatgattg gatggcaccg gtggtgaatc ctgatgagtt ttccccggcg
     1561 gtttgggcgt atatcggtga tgccgtttat gagctcttta cccggagtca gttgttgaag
     1621 gatggtccgg ccaagaccaa acagttacat aaggcgacca ttgcccgggt taaggcgtcg
     1681 tttcaggcgg aactgctcaa gcggttggaa cccgacttga ccaccgacga gctggaagtg
     1741 gtgaaaaagg ggcgtaatat caagagcggt catattccgg cgtacaccga tatgattact
     1801 taccggtata gtacggcctt tgaggccttg atcggctatt tatatctgac cggaagttat
     1861 caacggttac aggttatctt agccaagaca ttgatgaagg aatcggaaac cactggtatt
     1921 tcgggttgaa tcgcgtaccc gtcgagtatg cgcaaaataa ggcggttgaa ttcatgagcg
     1981 aacagattga aggcaggaat ccggtaattg aagcgctacg ttccggacat gagctggaaa
     2041 agctatatat tttaaaaggc ggagaaccga atggtccact ccgcgagatt ctggggcggg
     2101 ccagggagct taaaatcccg atacatccgg tggaagctca ggtacttgat aagatggcag
     2161 tcacccggaa tcatcaggga gtcatcgcac ttgccgccgg ttggaaatat gccacggtgg
     2221 agaagataat ggaacgggcc gcggaaagcg gagaaccgcc tttcgtactg ttcttggatg
     2281 gagtggcaga cccgcagaat ctcggttcga tcattcgaac ctccgaagct gccggagtac
     2341 acgggattat tattcccgaa cggagagccg ttggattaac cgctgcggta tgccgggctt
     2401 cggcgggagc ggtggagcac atgccggtgg ccagggtgac gaatctgaat cggacgatgg
     2461 aggagttgaa agcggccgga ctttggttta ccggcgccga tatggatgga gccgtcgtat
     2521 accatcgggc ggatctccgg gggccaatgg gactggtgat cggcggcgag ggcggtggtc
     2581 tctcccgttt ggtaaaagaa cattgcgatc agacggttcg cttgccaatg tggggcgcga
     2641 tcaattcctt gaatgccgct gtagccgcgg gtattctgtt gtatgagatc cgtcggcaaa
     2701 ggttgggatg agaaaaggca acagggaact cgcgcccctt ggggcacgcg gcaataggca
     2761 acaggtaatc gggaaccggt attacattgt tgcgtacgtg cggaaaacag gcgataaaca
     2821 aaagataaaa ggacaaacgc tttagtggct acctaaagat ttgtccttta ttttttgccg
     2881 gttgtctagt acctattgcc tgttgcctgt tttttatatg gaaatttgag tcaggctgat
     2941 acgattttgg agattgtttt tttccgggtt acatgataca attcaaaaat cgatggggag
     3001 attttccgcg aattgttggg aaacaaggag tggaccgctc ccttgacgtt catttgcata
     3061 ctattgtata atattactat gcaatgggtc aggcttcttt aattcaccgg ctgtcttttt
     3121 tgattgacga accgaaagct tcttaccgca acaatcttcc attgttcaga tttaagtgga
     3181 caagttcact ttccttcgcg agcataaagg aaatgtgcga tgttcacagt ttttctccgc
     3241 ttttcttcgg ggaataaccg gttttcaagc cgcgacattc atccgaatca tttcgtaccg
     3301 gttaatatgt atattttttc gctttgagtt gcaaatgggt ttctgtttat ttgtaatgcc
     3361 agtatcaaaa tttgttcata tctggaggcg aaataacgtg ggggctaggc cacagaggga
     3421 aacaaccgag atctatgatg agatgctgga tgaaatgatc gttgaggctg cccgtgaggg
     3481 tgacgatgcg gctcaagagt atctgatcaa taaatacaaa aattttgtcc gggcaaaagc
     3541 ccgttcctac ttcttaatcg gtgccgatcg cgaagacatc atccaggagg gcatgattgg
     3601 attatataaa gcgattcggg attttcgtaa tgataagttg gcatcgtttc gagccttcgc
     3661 cgaattatgc atcactcgtc agattattac ggcgattaaa actgcaaccc gtcaaaagca
     3721 tatcccgctt aactcctacg tatccctaaa caaaccaatc tatgatgaag agtccgatcg
     3781 tactttgctc gacgttatct ccggaacgaa agttaccgat cccgaggagt tggttattag
     3841 tcgggaggaa tttgtggata ttgagaataa aatgggtgag ttcttaagcg atctggagtg
     3901 gaaggtccta atgtcttatt tagacggacg ttcttatcaa gagattgcca aggacttacg
     3961 ccgccatgtt aaatccatcg ataacgcctt gcaaagagtc aagcggaaac tcgaacgcta
     4021 tctcgaaaag cgtgaagaaa taagttctct gtagacacac gccagcgaat gcacagttta
     4081 gaaatggaat aaaaaggttt gaaaaatcgg ttgacattcc acctgtctat gtattataat
     4141 tctatttgcg ttcggcaatt ggaggggttc ccgagtggcc aatgggggca gactgtaaat
     4201 ctgttggttg acgccttcga aggttcaaat ccttccccct ccaccattta aaagttcgaa
     4261 gttcaaagtg agaagtgaaa agttggtaag catgatagaa cttgaaactc tgaacgcgga
     4321 actcggaact taacaatcaa ataacatcgc ggggtggagc agttggtagc tcgtcgggct
     4381 cataacccgg aggccgaagg ttcaaatcct tcccccgcaa ccaattttaa aattcttgtt
     4441 ttacttcaat aattgccgcc cacatagctc agtcggtaga gcgcatccat ggtaaggatg
     4501 aggtcatcag ttcaattctg attgtgggct ccatttcaaa cttctgaacc cgaaagggtt
     4561 tttatttttt atccggtgaa gtttttgttt ccgaagattc cctgccggca ttctccctaa
     4621 aaaacattcc tgatactact ccaaaacaaa tggttttgtc tgggtataaa tgcctcaaat
     4681 aacggctagt ttatataatg gttaaaatcg gatgattttt tatgcactat agtttcgcca
     4741 ataatcgaat cgggttccaa gaaggaatat tcgttcccgt gtagaataga tcgctgttta
     4801 tcaatacctt tgcgatttaa aggaggagca agaaaatggc gaagcaaaaa tatgaacgga
     4861 ctaagccaca cgtcaacata gggacaatcg ggcacgtcga tcacggcaag accacaacca
     4921 ccgcggcgat taccctggta ttatccaaag cggggcaggc gaatttcgta aagttcgatg
     4981 agatcgataa ggcccctgaa gagaaagagc gcggtatcac catcaacact gcgcacgttg
     5041 agtacgagac cgagaaacgg cattacgccc acgtggattg cccgggccat gccgactacg
     5101 tcaagaacat gatcaccgga gcggcccaga tggacggggc gatcctggtg gtatccgcgg
     5161 ccgacggccc gatgcctcag acccgggaac acatcttgct tgcccgtcag gtgggagtgc
     5221 cctacatcgt ggtgtggctg aacaaggcgg atatggtgga tgatccggaa ctgatcgaac
     5281 tggtcgagat ggaagtccgg gatctgctgg cccagtatga attccctggc gacaagattc
     5341 cgattgtccg cggttctgga ttaaaagctc tggaatgcgg ctgcggcaag cgtgaatgcc
     5401 aatggtgcgg acggatttgg gatctgatgg atgcggttga ttcctacatt ccgactccgg
     5461 aacgcgccac cgacaaaccg ttcctgatgc cggttgagga tgtattcacc attaccgggc
     5521 gcggcactgt tgccaccgga agagtggagc ggggcgccgt gaaggtgggc gatgaggttc
     5581 aggtcattgg gttgcaaccg gagattaaga agaccatcgt taccggcgtg gagatgttcc
     5641 ggaagctgct ggatcaagct gtggccggag ataacatcgg tgtactgctc cgcggtattg
     5701 accggaaaga catcgaacgc ggccaggtgc tggcgaaacc gggttcaatc accccgcacg
     5761 tgaagtttga aggagaagtt tacgtattga ccaaagaaga aggcggacgt catactccgt
     5821 tcttcaatgg ataccggccg caattctact tccggacgac cgatgttacc ggagtggcgc
     5881 atctgcctga gggagtggag atggtgatgc cgggcgacaa catcaagatg acgat
//
LOCUS       DASVMB010000291         6711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_700742,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000291 DASVMB010000000
VERSION     DASVMB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700742"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VF531_08380"
     CDS             complement(<1..469)
                     /locus_tag="VF531_08380"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HEX7714026.1"
                     /translation="MESGMIVWGKVNFLQVIQVYLDQPLLGGFLGWLQDGALLWMVSQ
                     IGAAHVRFHRLILGGLIGGLFQFLLEINIATGGIVNPWILTPSVYLLVVPALMVMVAF
                     FPVPFRRFLRILGYVYLLSFLLSGFHWGIDILNQRFFHWEIALWWRFLMHLTLI"
     gene            complement(849..1892)
                     /gene="ftsZ"
                     /locus_tag="VF531_08385"
     CDS             complement(849..1892)
                     /gene="ftsZ"
                     /locus_tag="VF531_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7714027.1"
                     /translation="MLEFEVVNQQFASIKVIGVGGGGTNAVNRMIESKIQGVEFIAIN
                     TDGQALNRSNADIRLRIGDLLTKGLGAGANPEIGQRAALESKDELMGVLQGADMVFIT
                     AGMGGGTGTGAAPVIAQMAKENGALTVGVVTKPFTFEGKIRAAQADKGLASLKENVDT
                     LITIPNERLLQIVDKNTSIIDAFRMADDVLRQGVQGISDLITITGLINVDFADVKTIM
                     SQAGSALMGIGNAKGENRAEIAAKTAVQSPLLETTIEGSKGVLLNITGSSNLSLHEVN
                     VAAAIVTEAADPDANIIFGAVIDESLGDEIRVTVIATGFEQRRARRVGLEELEIKPFI
                     SEDLEIPAFLRKK"
     gene            complement(2114..2833)
                     /locus_tag="VF531_08390"
     CDS             complement(2114..2833)
                     /locus_tag="VF531_08390"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX7714028.1"
                     /translation="MGSKTNFSSIFAGWFIILVGLIFFNSGIFSIRNIVVTGNQTIAS
                     EDIIRATRLNLDKNIFQIDRAKIKSDILANPAILSVELHYQFPYTLEIRVRERQPFCL
                     LMYRDNLLVLGEDGVIIRVKNDNEPIKLPVVSGIKVNHLRIGSRVVDPRFQTAQEIWR
                     LADDYLRRTLSEIDLQDYQLYLDLPKSHHTLKVELGDANQLEEKIAKNLRSILSHTGP
                     DELIKIDLRVPSIPTTIKNPG"
     gene            complement(2858..3634)
                     /locus_tag="VF531_08395"
     CDS             complement(2858..3634)
                     /locus_tag="VF531_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714029.1"
                     /translation="MSVGGIILTICINLIVICALYIFQKRDIDRRLKHVKTEIQELED
                     LVSAIIAEFEAIADEVDQDSVGAGGAEEAAMNEGISGVQNGDEETVTERIIAEVEAAL
                     ENPDTIAAPSQILSEREGGGDSLKDQDFEAEQTVLESTHSESSSSTVGVVIPEEQQVS
                     DEETISCPTNVAKAVSSNHTERDLQVPQDESEPQLPLTFSGPEPVAPSSPTQPVISDP
                     RHRRVYELWRRGLTVEEIAKELGTGRGEIQLLLYQWTVKR"
     gene            complement(3722..4048)
                     /locus_tag="VF531_08400"
     CDS             complement(3722..4048)
                     /locus_tag="VF531_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714030.1"
                     /translation="MLKPLDLQTVLPRSVDIQRMQQIQYAQPVIDQQEVTREVVKRNQ
                     LKQEQIQNNQATAETNPIRTNENNPQNRERRYRRFRQTRQGASQESESKTPAEPERGQ
                     HIDIKF"
     gene            complement(4076..5503)
                     /locus_tag="VF531_08405"
     CDS             complement(4076..5503)
                     /locus_tag="VF531_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX7714031.1"
                     /translation="MAENGSNSIPNTAGSTDRDGRCRVQVSNNSMEAVLIVEAPVGRG
                     KWPTCEDALAALRGENITFGIDPRAIDEVIKNQLLKPTSVAKGRPPVAGRDAELKFLF
                     ETGIFRKASLEDETGKIDFREVQTVQNVTLGQTIIEKIPATPGEPGCDVRGKDIPAAP
                     GKDKTIKTGKNVAWSEDGLALRATSGGEPTYINGKVSVLPVKELKSDVNFDTGNIVFS
                     GNLTIRGNVESGFKVEAEGDITIYGNVDSADIKCGGNLFIQGGISGMDKTEIYCVGDF
                     SAKYIEHASVVCEGNLTVREAIMYCQINTDNKVTVEGGKGLLVGGLVRSGEEISAKTI
                     GSRLGTVTELEVGIKPRTKLEFQELEEQIKNNTESLEKAEKAIVILTKIPNLPDDRKE
                     MLQNLVKTAYALKSQIAEAETRRQELLDEIQNRSKDKGRIRVKELMYPGVRVTMGKTT
                     IMIQDEIKYAMLVYNEGEIQIQPYR"
     gene            complement(5507..6301)
                     /gene="whiG"
                     /locus_tag="VF531_08410"
     CDS             complement(5507..6301)
                     /gene="whiG"
                     /locus_tag="VF531_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX7714032.1"
                     /translation="MSVDKAGQNEENLWQDYLGTKSPQAREAIILRYSPLVKYVAGRV
                     AIGLPSNVEFDDLVSFGVFGLMDAIEKYDPSRGIKFETYAIARIRGAILDGLRSNDWV
                     PRSVRQKARELERACAELENRLGRSATDQEISEAMDLNLQEFYQLLSEVSCTTLSSLD
                     ELWLVHAGDDDTVRVLDLVRNDESEDPLEQVEMEEIKTTLATAIDCLPERERMVIALY
                     YYEGLTLKEIGEIMEISESRVSQIHTKAIFRLRGRLNRWRKSIAEV"
     gene            complement(6420..>6711)
                     /locus_tag="VF531_08415"
     CDS             complement(6420..>6711)
                     /locus_tag="VF531_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX7714033.1"
                     /translation="ADTAVPLLIREMEKMGVRRERLIVKIVGGAQMFCGNGYEDHICI
                     GNKNIIAVETICQQLGLFISGRSVGGNSGKSVFLDLETGEVQVKTLTEYIKI"
BASE COUNT         1733 a         1803 c         1375 g         1800 t
ORIGIN      
        1 aaatcaaagt cagatgcatt aaaaagcgcc accataaggc aatctcccaa tggaaaaacc
       61 gttgattcag aatatcgata ccccaatgaa aaccggagag taaaaaggag agtaagtaaa
      121 cataaccgag tatcctcaaa aatcgccgga agggaaccgg aaagaaagca accattacca
      181 tgagagccgg taccactaac agataaacgg aaggtgtgag tatccatggg ttgacgatcc
      241 ctccggtggc gatattgatc tcaagtagaa actgaaagag accgccaatc agccctccaa
      301 gaattaagcg gtggaaacgg acatgggcag ccccaatctg ggagaccatc cacagcaaag
      361 ccccgtcttg cagccaacct aaaaaacctc ccaataacgg ttgatccaga tagacctgta
      421 ttacctgcaa aaagttcacc ttcccccaaa cgatcatacc gctttccaaa cgaatttcct
      481 ttttaaatgt ccggcgtaaa acaatgtcga ttcatcactg tgttagcagg tcgaagtgat
      541 tcaatccgtt agtttccggc ttaccaccgg aagttcccga aatcccccgg ctttttgtgc
      601 tttttaacga aatccccgta aaaactcgaa aagcgttcag ttattaactg aacgcttttc
      661 aaatcatatt tacttgaaca ctttaactcg ggataaacct tcgcatctca agccactggt
      721 taaaaccagc ggctgtaata attcttacca gttgaaaccg gtatcgttcg ttgcagcaaa
      781 gcattgcact cgcgacagaa tagtcaaaac ggatagtcat cataacttat aactaacaac
      841 cagcgcccct atttcttccg gagaaatgcc gggatctcca aatcttcact aataaacggc
      901 ttgatctcca gttcctccag tcctaccctg cgggcacggc gctgttcaaa cccggtggca
      961 ataactgtca cccgaatctc atcgcccaac gattcatcga taaccgcgcc aaaaataatg
     1021 ttcgcatctg gatccgcagc ctcggtaaca attgctgcag ctacattcac ctcatggagg
     1081 cttaaattac tgcttcctgt aatattcaac aatacgccct ttgacccttc gatagtcgtc
     1141 tcaagcagcg gactttgcac tgccgttttg gctgcgatct cggcccggtt ctctcctttg
     1201 gcattcccga ttcccattaa ggctgatccg gcttgactca tgatggtttt cacatccgca
     1261 aaatccacat tgatcaggcc ggtaatggta atcaaatcgg agattccctg taccccctgg
     1321 cgtaatacat catccgccat ccgaaaggcg tctataatgg aggtgttctt atcgacaatc
     1381 tgaagtaaac gttcgttggg gatggtaatc agggtatcca cattctcttt caaactggct
     1441 aaccctttgt cggcttgggc ggcacgaatt ttaccctcaa atgtaaaagg cttggttaca
     1501 acgccgacgg ttaacgcccc gttttccttg gccatctgtg cgatcaccgg tgcagctccg
     1561 gttccggttc caccacccat tcctgctgtg atgaagacca tatccgctcc ttgcaagacg
     1621 cccatcagtt catccttgct ttctaacgcg gcgcgttggc cgatttcggg attagcccct
     1681 gctcccagac ctttggtgag aaggtctccg attcgcaagc ggatatccgc attggagcga
     1741 tttaatgcct gaccatcggt gttgattgcg ataaattcca ctccctgaat tttggactca
     1801 atcattcgat tgacggcatt agtaccgcca ccgccaacgc cgatgacttt gatcgaggca
     1861 aattgctgat tgaccacctc aaactcaagc attaaaaaac tccccctgta aaagtccaaa
     1921 cggatctcaa gttgcacaga gaccctttta tttacatcaa aatgtcacct gaccggtatt
     1981 aagaggttct aaactagtct gaaactagtt taaactaaaa aagatcgaca tcttacaccg
     2041 gcgtaataga atactattct gcacacagat agcattaccc tgccgattgt tcaaaatttc
     2101 tcggataaat tgtttagccg ggattcttaa tggtagtggg tatggaagga acccgcagat
     2161 cgatcttgat cagttcatcc ggtcctgtat gagagagaat agaacgaaga ttcttggcga
     2221 tcttctcttc taactggtta gcatccccca gttcaacttt tagcgtatga tgcgatttcg
     2281 gaagatcaag gtataattga taatcttgga ggtcaatctc gcttaaggtt cggcgtaaat
     2341 aatcatcggc caggcgccag atctcttgag ccgtttgaaa ccgggggtct acaacccggg
     2401 aaccaatccg gagatgattt accttaatgc cggagactac gggcaattta atcggttcat
     2461 tatcattctt gacccgaata ataaccccgt cctcccccaa gaccaacaga ttatcccggt
     2521 acatcaaaag acaaaatggc tgacgctccc ggaccctgat ctccaaagta taaggaaatt
     2581 ggtaatggag ctcaaccgat agtatggcgg gatttgccaa aatgtccgat ttaatttttg
     2641 cccgatcaat ctgaaatata ttcttatcca aattcaaccg ggttgctcta ataatatctt
     2701 cactggctat cgtttgattc ccggtaacga caatattgcg aatggagaag attccggagt
     2761 tgaaaaagat taagcccacc aaaatgatga accatcccgc aaagatactg ctgaaattag
     2821 ttttacttcc cacgggattt gcgcctttca ataataatta acgtttaaca gtccattgat
     2881 atagaagcag ttgaatctca ccccgtccgg tacctaactc cttggctatc tcctcgaccg
     2941 tcaaaccccg ccgccacaat tcataaaccc gccggtgcct gggatcactg ataacaggct
     3001 gagtaggaga agagggagct accggttccg gcccggaaaa cgtcaacggc agttgcggct
     3061 cactttcatc ttgcggcacc tgcaaatccc gttccgtatg attagaagat actgccttcg
     3121 ctacatttgt aggacaggaa atcgtttcct catcggaaac ttgctgttcc tccgggatta
     3181 cgacgccaac cgttgatgag ctactctccg aatgggtcga ttccaaaacc gtttgttcag
     3241 cttcgaagtc ctggtctttg agggagtctc cgccgccctc ccgttcagat aaaatttggc
     3301 ttggagcggc aatcgtatca ggattctcaa gtgccgcctc tacctcggcg ataattcgtt
     3361 cggtaaccgt ctcttcatca ccattttgga cgccggagat accctcattc atggcggctt
     3421 cttcggcacc acccgcccct accgaatcct ggtctacttc gtcggcgatc gcctcaaact
     3481 cagcaataat cgcactaacc agatcctcga gttcctgaat ctcagtcttg acatgtttga
     3541 gtctccggtc aatatcacgt ttctgaaaga tatataaggc gcagataaca atcaggttga
     3601 tgcaaatagt aagtataatc ccgccgactg acacgatggt tttgcctcct cgactggtcc
     3661 ggaatgctcc gtgaaccctg gtcgatcttt aactcaaaaa atagtaaaaa aaaacactcg
     3721 gctaaaattt gatatcgata tgctgccccc gctcgggctc agccggtgtt ttcgattcgg
     3781 attcctggga agcaccctgt cgcgtttgac gaaagcggcg gtaacgccgt tcgcggtttt
     3841 gaggattgtt ttcattggtg cggatggggt tggtttctgc ggtagcctgg ttgttttgaa
     3901 tctgttcctg tttgagttgg ttccgcttta ccacttccct ggtgacttcc tgttgatcaa
     3961 tcaccggttg ggcgtattga atctgctgca tacgctgaat atcaacggaa cgaggcaata
     4021 cagtttgaag atccaatggt tttaacactc gaaaaccacc acccaaagcc acattttatc
     4081 tgtagggttg aatctggatc tcgccctcat tataaacaag catggcgtac ttgatctcat
     4141 cctgaatcat aatggtggtc ttacccatcg taacccgaac tccgggatac attaactctt
     4201 tcacgcggat ccggccctta tctttggaac ggttttgaat ctcatccagg agttcttggc
     4261 ggcgggtttc cgcctcggcg atctgtgatt ttaaggcata agcggtcttt accaaattct
     4321 gcaacatctc ttttctgtca tcaggcaggt tggggatctt ggttaagatc acgatggcct
     4381 tctcggcttt ttccagactc tcggtattat tcttgatttg ctcttccagt tcttgaaact
     4441 ccagcttagt ccggggtttg atgccgactt ccaattcggt caccgttcct aaacgagaac
     4501 caatggtttt agcggaaatc tcttcaccgg atcgaaccag ccctccgact aacaatccct
     4561 tgccgccttc caccgttact ttgttatcgg tattaatctg acaatacatg atcgcttctc
     4621 tgacggtgag attgccctcg caaaccaccg aggcgtgttc gatatatttg gcggaaaaat
     4681 cgcctacaca gtaaatttcg gttttatcca taccggaaat gccgccctgg ataaaaagat
     4741 taccgccgca tttgatatcc gccgaatcga cattcccgta aatcgtaatg tcgccttccg
     4801 cttcgacctt aaaaccgctt tccacattgc cgcgaatcgt tagattgccg gaaaagacga
     4861 tattgccggt gtcaaagttg acatcgcttt ttaattcctt taccggcagt acactgactt
     4921 ttccattgat gtacgtgggt tccccgccgc tagtagcgcg aagtgctaaa ccatcctcgc
     4981 tccaagccac gtttttaccg gtcttaatcg ttttatcctt gcctggagcc gcggggatat
     5041 ccttgccccg gacatcacaa cccggttccc cgggagtagc cggaatcttc tcgataatcg
     5101 tctgaccaag agttacattt tgtacggtct ggacctcacg aaagtcgatc ttcccggtct
     5161 catcctctaa agaggccttc cgaaaaattc cggtctcaaa taaaaatttg agttcggcgt
     5221 ccctgcccgc gaccggtgga cgccctttgg caaccgatgt tggcttaagc aattgatttt
     5281 taataacttc atcaatagcc cttggatcaa taccaaaagt gatgttttca ccacggaggg
     5341 cagccaatgc atcttcacag gtaggccatt taccccgacc aacaggggcc tcgacaatta
     5401 atacagcctc catggaattg tttgatactt gaacccggca acgcccatcc cgatcggtac
     5461 tcccagctgt attaggaatc gagttcgagc cattttccgc catcgtttac acctccgcga
     5521 tcgatttacg ccaacgattt aaacgtccgc gtagtctgaa tattgccttt gtatgtatct
     5581 gagatacacg cgactccgaa atctccataa tctcgccgat ctccttgagg gtaagtcctt
     5641 cataataata taacgcaatg accatccgct cccgttccgg caaacaatca atggctgtgg
     5701 ccaaggtggt cttaatctcc tccatctcca cctgttctag cggatcttca ctctcgtcgt
     5761 tccgaaccag atcaaggaca cggacggtat catcgtcgcc tgcgtgaacc agccataact
     5821 catccagtga acttagcgtg gtacaactta cttccgaaag taactgataa aactcttgta
     5881 aattgaggtc catcgcctca ctaatctcct gatccgtagc ggacctgcca agccgattct
     5941 caagctcagc acaggcacgt tcaagttccc gggccttctg tcttacagat cgggggaccc
     6001 aatcattact ccgtaaacca tccagaatcg caccccggat tcttgcaatg gcataagttt
     6061 caaatttaat tccacgcgat ggatcatact tctctattgc atccatgagc ccaaatactc
     6121 caaaactgac caagtcatca aattcgacat tggacggtaa accaatcgcc accctgcccg
     6181 caacgtactt caccaatggt gaatacctta aaataatggc ttctctggct tgaggagatt
     6241 tggttcccag ataatcctgc cataaattct cctcattttg tcccgctttg tcgactgaca
     6301 ttgctaccct ccttacacgg gtttacccgt accatccggt ggctaaattc ccttccttga
     6361 tcatcctggt acagtcgcgc gtgaaagctt attttataaa ttgcaaagct cggtttggat
     6421 cagatcttga tatactcagt taatgttttt acttgaacct caccggtctc caaatccaaa
     6481 aaaacacttt tcccgctgtt tccgccaacg cttcgaccgg aaataaacaa tcccagttgt
     6541 tggcatatgg tttccacagc aataatattt ttgttcccga tgcagatatg atcttcgtaa
     6601 ccattcccgc aaaacatttg ggcaccgccc actatcttta caatcagccg ttctcgccta
     6661 actcccatct tttccatctc ccgaatcaac aatgggacgg cggtatcggc a
//
LOCUS       DASVMB010000292         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_36855,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000292 DASVMB010000000
VERSION     DASVMB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36855"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF531_08420"
     CDS             <1..320
                     /locus_tag="VF531_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX7714034.1"
                     /translation="EKIIGAELELDPARQVEIVREALLRAANAESVEVRVHPDDLAIC
                     EQNIQRLQEAFADPKPIRFTADPGLAPGSCFIETNLGNVDARLKSELDMILAELLKAR
                     QIG"
     gene            317..1693
                     /gene="fliI"
                     /locus_tag="VF531_08425"
     CDS             317..1693
                     /gene="fliI"
                     /locus_tag="VF531_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX7714035.1"
                     /translation="MSSSSQACENSSRPAPFDAESYLKILRNFDPIKRTGRVAQIVGL
                     TVEGDGPSANMGEHCLIIVKGMAEPLSAEVVGFKAGRVLLMPLGDMSGISPGCEIIAT
                     GQKLQVEVGSGLLGRVLDGLGRPMDGQGPLLTGEYYPLTAIPPNPLTRNRINKTLAMG
                     IRAIDTLLTCGRGQRVGIFAGSGVGKSTTLGMIARNTEADINVIGLIGERGREVRDFI
                     ERDLGPVGLARSVVVVATSDQPALVRIKGAMVATAVAEYFRDQGKDVMLMMDSVTRFA
                     IAQREVGLAVGEPPTTRGYTPSVFAMLPQLLERSGTSDRGSITGLYTVLVEGDDMNEP
                     IADAVRSILDGHIVLSRNLAHLNHYPAIDVLSSVSRLMSELATDSHKKAAGVLRTMLA
                     TYQNNEDLINIGAYAPGSNSQIDEAIRMIGPINDFLCQRVDEKAPFQETLTRLITMMG
                     GNAGNGKV"
     gene            1680..2189
                     /gene="fliJ"
                     /locus_tag="VF531_08430"
     CDS             1680..2189
                     /gene="fliJ"
                     /locus_tag="VF531_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX7714036.1"
                     /translation="MAKFKFKLETVLKVKTRVEELRQKELKETEVRRDAARIELARRE
                     KEAADTALTYREQLRANLDIRRANDYARFLTWLNHQVDLAVRELQQCEAAVTAARERL
                     ILAAKEKKVLEKLKQKAYEAYKAEELNAEIKFLDELGTEQYLRQQIAAEQMTGRQMAG
                     EQNMGKRRG"
     gene            2255..2848
                     /locus_tag="VF531_08435"
     CDS             2255..2848
                     /locus_tag="VF531_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714037.1"
                     /translation="MRRFLFRSSQVLLVFGLLYFIFWALTQFGLVDTKTLVLQTVSLL
                     PGMEDLPVNYELGGKRDDLLKQRERDLARREARLADAQARLAADRDGFTNEKNNWLNT
                     HPQATAVPNSTPGTLVPGQLNPVKSADAKIKNYLEMIGKMQPQQAAVVIQKLPEPTVF
                     AIFDQLSNYQVRKIMEHLPPDYLAKLTQDRLIQYRNI"
     gene            3025..>4227
                     /locus_tag="VF531_08440"
     CDS             3025..>4227
                     /locus_tag="VF531_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714038.1"
                     /translation="MAGQVNNLSQLLVDTVRQFENQVPAKAEEWMGANRGEFKNILAT
                     YDHPFAKAPANHPFDKIQAKAVRNSDRPGSAVADRPETTATAEKGEQPKPREDAANSR
                     AADKPSSDEARVTNSSTQPASDEAQKAPSGAKEAVTETSNDSEPATSETDIPPELAVA
                     ISDGSTGIPAVTEADVPTENENAGDDPDNQSATGMNPTDSVQTALLMSLLSVVLPGAD
                     STAMVTNPGLVAMMTSESAGRPDGTGKTAETKLSAPGAQSTLAAVLEESGITSDQTVK
                     TATPQGSKDVKLSQLFAAKSGDGSGETGVSGQGLTNSNLNPDQGDSGIRFDLSDVAKG
                     HTPTGLVQPFTESGGESKPLNLTVIQSQLAVGEGDKEATSIQLMSAPLKQQPVESGMT
                     QEVSGKGAP"
BASE COUNT         1022 a         1044 c         1249 g          912 t
ORIGIN      
        1 cggagaagat catcggtgcc gaactggagt tggatcctgc ccggcaagtg gagatcgtgc
       61 gtgaggcgtt acttcgggcc gcgaatgccg aatctgtgga agtccgggtt catccggatg
      121 atttggcgat ctgcgaacaa aatatccaac ggttgcagga ggccttcgct gatcccaaac
      181 caatccggtt cacagcggat cccgggctcg ctccgggaag ttgttttatc gagaccaatc
      241 tggggaatgt cgatgcccgg cttaaatcgg agttggatat gatcctggcc gaactcttaa
      301 aggcgaggca aatcggatga gtagttcatc gcaggcgtgt gagaattcct cccggccggc
      361 cccttttgat gccgaaagtt atttaaaaat actgcgcaat ttcgatccga tcaaacgtac
      421 gggaagggtc gctcagatcg taggtttaac cgtggaaggc gacggcccct cggccaatat
      481 gggtgagcac tgtctgatca ttgttaaagg gatggccgag ccactctcgg ctgaagtggt
      541 ggggtttaag gccggccggg ttttgttgat gccgctcggt gatatgtcgg ggattagtcc
      601 cggctgcgag atcattgcca ccgggcaaaa actccaggta gaggtaggct ctggattatt
      661 aggccgggtt ttggacggtt taggccggcc catggatgga caaggaccgc ttttgaccgg
      721 ggagtattat ccgttaaccg ccataccgcc caatccattg actcgcaacc ggatcaacaa
      781 gacactcgcc atggggattc gggcgattga taccttgttg acctgtggcc gcggtcagcg
      841 ggtcggaatc tttgccggca gcggcgtcgg aaaaagtaca actctgggga tgattgcccg
      901 caataccgag gccgacatca acgtaatcgg cttaatcggt gaacgcggcc gcgaggttcg
      961 cgattttatc gagcgggatt taggtccggt gggattggca cgttccgtgg tggtggtcgc
     1021 cacctccgat caaccggctt tggtacggat taaaggggcc atggtggcta cggcggtagc
     1081 cgagtacttc cgggatcagg gcaaggatgt catgctcatg atggattcgg tcacccggtt
     1141 cgccatcgcc cagcgggagg tgggactggc cgtcggtgaa cctcctacca cccggggcta
     1201 taccccttcg gtttttgcca tgcttcctca gctcttggag cgttccggaa cttcggatcg
     1261 gggcagcatc actgggttat ataccgtgct ggttgagggg gatgacatga acgaacccat
     1321 cgccgacgcg gtccggagta ttttggacgg acatatcgtt ctctccagga atttggctca
     1381 tttaaaccat tatccggcca tcgacgtttt aagcagtgtt agccggttaa tgtccgaact
     1441 ggccacggac agtcacaaga aggccgcagg agtgctccga accatgctgg ccacttacca
     1501 gaataatgag gatttgatca atatcggagc ttatgctccg gggagcaatt ctcagattga
     1561 cgaggccatc cggatgatcg gcccgatcaa cgatttcctt tgtcagcgtg ttgatgaaaa
     1621 ggctcctttc caggagacgc ttacccgttt gatcacgatg atgggtggga atgccggcaa
     1681 tggcaaagtt taaattcaaa ctggagactg tgttaaaggt caagacccgg gtggaggagt
     1741 tgcgccaaaa agaattgaaa gagacggaag tccgtcgtga cgcggcccgg atcgaactgg
     1801 cgcgccggga aaaggaagcg gccgatacgg ccttgacata ccgtgaacaa ctccgggcca
     1861 atctggatat tcggcgcgct aatgattacg cccggttctt gacttggctc aatcaccagg
     1921 ttgatctggc cgttcgcgag ttgcagcaat gtgaggctgc ggttaccgcg gcccgggaac
     1981 ggttgatcct cgcggccaag gaaaaaaagg ttctggaaaa attgaagcaa aaggcctatg
     2041 aggcctataa agcagaggaa ttgaatgcgg aaatcaaatt cctggatgaa ctgggcaccg
     2101 aacaatatct ccggcaacag atcgcggcgg aacaaatgac cgggcggcaa atggcaggag
     2161 agcaaaacat ggggaagcgg cgggggtagc cgcgccgatt gccggttcca agaccggtcg
     2221 cgggagaatg cggataggga gggaagagta accgatgcgc aggtttttgt ttcggtcatc
     2281 acaagtgtta ttggtgttcg gattgctcta ttttatattt tgggcgttga ctcaattcgg
     2341 cttagtggat acgaagacac tggtattgca gactgtcagc ctgctcccgg gaatggagga
     2401 tctgcccgtc aattatgagc tgggtgggaa acgtgacgat ctcctgaagc agagggaacg
     2461 ggatctggcc cggcgcgagg cgcgattggc cgatgcccaa gcccgtttgg cagcggatcg
     2521 ggacgggttt accaacgaga agaataactg gttgaatacc catcctcagg ccaccgccgt
     2581 tccaaattcg actccgggta ccttggttcc gggccagttg aatccggtta aatcagccga
     2641 tgcaaagatc aagaactatt tggagatgat cggcaagatg cagccccagc aagcggcggt
     2701 agtgattcag aaactgcctg aaccaacggt gtttgcgatt tttgatcagc tctcaaacta
     2761 ccaggtacgc aagatcatgg agcatttgcc cccggattac ctggccaagc tgactcagga
     2821 tcgcctgatt cagtatagaa atatttagca cggtaagttc gaatgttctt ttactgccgc
     2881 cctccggatt tctgccggga gcggcttttt ttattctttt caaaacgata atttacagaa
     2941 ggcaggtttg gtctgtcgat tcctataatc tcttttccgg aatcatccga taacgtaata
     3001 agaatattga aaggaggtga gaacatggct ggtcaagtga ataatctttc ccaacttcta
     3061 gtggacacgg tcaggcagtt tgaaaaccaa gtacctgcca aggcggaaga atggatgggc
     3121 gctaatcgcg gtgagttcaa gaatatctta gccacttacg atcatccgtt cgcaaaggca
     3181 ccggcaaacc accctttcga caagatacag gccaaggcgg tccggaattc agaccgtccg
     3241 ggaagtgccg ttgccgacag gccggaaaca accgctaccg ccgaaaaggg tgaacaaccg
     3301 aaaccgcgag aggatgctgc aaattcccgg gctgccgaca agccttcatc cgacgaggcg
     3361 cgcgtgacga attcatctac gcagcccgca tccgacgaag cccagaaggc accatccggt
     3421 gcgaaggaag cagtcaccga aacctcgaac gactccgaac ctgcgacctc cgaaaccgac
     3481 attccgccgg aattggcagt cgcaatttcc gatggaagca ccgggattcc tgcggttaca
     3541 gaggcggatg ttccaaccga gaatgagaat gccggagatg atccggataa tcaatcggct
     3601 acgggcatga atccgacgga ttcggttcag accgcattgt taatgagttt attgagtgta
     3661 gtgcttcccg gggccgattc aacggctatg gtgacgaatc ccggattggt ggcgatgatg
     3721 acttcggaat ccgccggacg accggacgga accggtaaaa ctgcggaaac caaactgtcc
     3781 gctccgggtg cacaatcaac cttggctgct gttttagagg agtcaggaat tacttcggac
     3841 caaaccgtta aaaccgcgac cccacagggg agcaaggatg tgaagctctc tcaattgttc
     3901 gctgcaaaat ccggtgatgg gagcggagaa accggagtat ccggtcaagg cctgactaat
     3961 tccaatttga acccggatca aggcgactcg ggaatccggt tcgatctttc ggatgttgcc
     4021 aagggacaca ccccgacagg tttggtgcag ccgttcaccg aaagcggggg tgagagtaaa
     4081 cccttaaatc tcacggttat ccaaagccaa cttgccgttg gggaaggaga caaggaggct
     4141 accagtattc agctgatgtc cgctccgctt aaacaacagc cggtggaatc ggggatgacc
     4201 caggaggttt ccggcaaagg ggctccg
//
LOCUS       DASVMB010000293         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_181718,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000293 DASVMB010000000
VERSION     DASVMB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181718"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VF531_08445"
     CDS             <1..1511
                     /locus_tag="VF531_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7714039.1"
                     /translation="ESESSGRKYVNLSAAGQYVQWTLSQPANAIVIRYNMPDASGGGG
                     ITANLSFYVNGTHNRDISVTSKYAWVYGGYPWDNNPANGNAHRFYDETRVLLGTTLAA
                     GTVIKLQKDSGDTAAYYNIDLIDMENVGSALTQPANSLAITSYGAVANDANDDTAAFN
                     SVVSAAISQGKSVWLPAGTFILNSRPNSFSGVTIHGAGMWYTVLSGSGASFSPNGNNC
                     KFYDFALFGNTTQRIDSALETGFDGNLGTGSVIQNIWMEHLKCGIWANNGTNGLLVNG
                     CRIRNTFADGINLYNGTKNTTVQNCSLRNTGDDSIAIWSGGGNACTGNIVQSNMVQSP
                     YHANTIAVYGGSGNTVQNNYCYDTVQFGSGVYLANRFSVVAFSGTTTVSGNTLTRCGS
                     WDNDYSLPWGAIKLGAEENNMGGTFTISNNTLVNSLYSGILIIGPNSCNGAIFSNINI
                     ITAGTYGIEVRNNAYGSATFTSVAVSGALSGGLNNAAGSNFTINKGSGNSGW"
     gene            complement(1600..3045)
                     /locus_tag="VF531_08450"
     CDS             complement(1600..3045)
                     /locus_tag="VF531_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7714040.1"
                     /translation="MNIYELYLRSFYDSNGDGIGDFRGLAEKMDYLVDLGIDLVWFLP
                     ILKSQSFHGYSINDFFAVNPAYGSLEDLKAALQIGHQKGLKFILDLPLNHVGYNCEWF
                     KRALKGEKPYVDWFLWADETTDINEKRDWDPSRIWFKANGKYYYALFGPGSPDLNYAN
                     RDLWDMAKKIVTFWLEAGFDGFRFDAAPHIFDYDPQIGHFFKDHEKNIGFWREIVAWA
                     KTINPDAVMVSEVWDKKGVIDKYADIFEYLFNFPLAGTMKASIQMSSPKKFCLGLQRD
                     MADYFLAGSATPFHSCNFLTNHDMSRLLSELKNPNQAKLAYAILFTLPGAPCIYYGEE
                     LGMKGPISNVNNTEDAQDPLHWYENGFGPGQTEWKAYRHNHPFTKVSVEAQHPDEQSM
                     LNYVRSLTAFRKNNPWICAGRIKILQRNRRLVALSISDLFHEIVCYYNFSSKRVKVPG
                     GTSRKISGFGGYSMDRSHLIIDPYATVVIHK"
     gene            complement(3088..>4118)
                     /locus_tag="VF531_08455"
     CDS             complement(3088..>4118)
                     /locus_tag="VF531_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX7714041.1"
                     /translation="YHQGVEMLIQISRFYASRVQQNPDTLQYGFYGVMGVDEFHMMVN
                     NNCFINYLAKKALEFTVAVIDEMKKDAPEALSAVIQKTGYNGMESEKWKQITANMVLP
                     QNKATGVYEQHDGFFTLPHLNIDAIPTDDFPLYNHWSYDRIFRHDMIKQPDVLMMLFL
                     YSQDFSIQTKRANYHYYEPKCIHESSLSPSIHSIIAAEIGELEEACEFFRFATRLDLD
                     DYNRNTWEGLHITSIAAAWMNIVYGFGGMRSDGPMLAFNPSLPPQWKSYSFRILYQNA
                     ILSLSVNQEHIFIKTNHHTPVTIMIYGNSYQIDDQGLKLNFQPNTQIKEAYHKYSTGA
                     RKLRMQEE"
BASE COUNT         1115 a          910 c          951 g         1142 t
ORIGIN      
        1 tagaatccga atcgtcgggt cgaaaatatg ttaatctgag tgccgcggga caatatgtcc
       61 aatggactct ttcccaaccg gccaacgcca ttgtaatcag gtataatatg ccggatgctt
      121 ctggtggtgg cggaattacc gccaatttaa gcttttatgt taatggaacg cacaatcggg
      181 atatttctgt aacatcaaaa tatgcctggg tctatggcgg atatccatgg gataacaatc
      241 ctgcgaacgg caatgcccat cgattctatg atgaaacccg ggtgttattg gggactaccc
      301 tggctgccgg cacagtgatc aaactccaaa aagattcggg agatactgca gcttactata
      361 atattgatct tatcgatatg gaaaatgtcg gttcggccct tactcaaccg gcaaattctt
      421 tggcgataac cagttatggt gcggtcgcaa atgatgccaa tgatgataca gccgctttca
      481 atagcgtagt ttccgccgcg atttcccaag gcaagtccgt ctggttgccc gcaggaacat
      541 ttatcctgaa ttccaggccc aatagttttt caggtgtaac cattcatggt gccgggatgt
      601 ggtataccgt acttagcgga agcggagcct cattctctcc gaatggaaac aattgtaagt
      661 tctatgactt tgcattgttt ggaaatacga cccagcgcat cgacagtgct ttggaaaccg
      721 gatttgacgg caatttagga accggttcag taatccagaa tatctggatg gagcatttga
      781 agtgtggaat atgggctaat aacggcacga acggactttt agtcaatggc tgtagaattc
      841 gaaacacctt tgcggatgga atcaatcttt ataatggtac caaaaacacc acagttcaaa
      901 attgcagcct tagaaatact ggagatgatt cgatcgccat ctggtctggc ggtgggaatg
      961 cttgtaccgg taatatcgtt caatcaaata tggttcagag tccgtaccac gccaacacaa
     1021 ttgcagttta tggcggttcg ggcaacacgg ttcagaataa ttattgttat gatacggttc
     1081 aatttggctc cggcgtttat ctggccaacc gttttagtgt ggtagcattc agcggtacga
     1141 cgacggtttc cggaaatacc ttaacccgct gcggatcctg ggataatgat tatagtctgc
     1201 cttggggagc cattaagctt ggggctgaag aaaacaatat gggcggaaca tttaccatca
     1261 gtaataatac gctcgtaaac agcctatatt ccggaatatt aatcatcggg ccaaattcgt
     1321 gtaatggcgc catttttagt aatatcaata ttattacagc cggaacctat ggaattgaag
     1381 tcaggaacaa tgcttatggc agtgccacat ttaccagtgt ggcagtttcc ggtgccctat
     1441 ccggcggttt gaataatgcc gcggggagta atttcacaat taataaaggt tccggcaaca
     1501 gcggttggta attcaaaact aaatggaaaa aaggggcagg tgagaacctc atttacccct
     1561 tttttcaatg atgaagatca tgcacgaatc cggttcatgt catttatgaa tgacaaccgt
     1621 cgcataggga tcgattatca aatgagatct atccattgaa tatcccccaa atccgctgat
     1681 cttccgggaa gtgccgccag gaactttcac cctcttcgag ctgaaattat aatagcacac
     1741 gatttcgtga aagagatcgg agatactcag tgccaccaat ctgcgattcc gttgcagtat
     1801 tttgatcctt cccgcacaga tccatgggtt atttttgcgg aaggcggtca acgatcgcac
     1861 ataatttagc atggattgtt catccgggtg ctgtgcttca acggatacct ttgtaaaagg
     1921 gtggttatgg cgataggctt tccattcggt ctgtcccggt ccaaacccat tttcatacca
     1981 atggagggga tcctgagcgt cttcggtatt gttcacattg gaaatgggtc ccttcatccc
     2041 cagttcttct ccataataaa tacagggcgc tcccgggagg gtaaaaagga tcgcgtaagc
     2101 gagtttggct tgattcggat tcttcaactc cgaaagcagg cgggacatat catgattggt
     2161 caaaaaattg cagctgtgga agggagttgc ggatccggcc aaaaagtaat cggccatatc
     2221 cctttgaagg cccaagcaaa attttttggg actagacatt tgaatgctcg ccttcatcgt
     2281 gccggccagc gggaaattga agagatattc aaatatatcg gcatacttgt cgatgactcc
     2341 ttttttatcc caaacctcac tgaccatcac cgcgtccgga ttgatggttt tagcccatgc
     2401 cacgatctcg cgccaaaacc cgatgttttt ctcgtggtct ttaaaaaagt gtccaatctg
     2461 cggatcataa tcaaagatat ggggtgccgc atcaaaccgg aaaccatcga aacctgcctc
     2521 cagccaaaat gtcacgattt tcttggccat atcccacaga tcgcggttgg cgtagtttaa
     2581 atccggcgaa ccggggccaa acaaggcgta ataatacttg ccattggcct tgaaccaaat
     2641 ccggcttgga tcccagtctc tcttttcatt gatatcggtc gtttcatccg cccataaaaa
     2701 ccaatcgacg tatggtttct ccccctttag cgcccgtttg aaccactcgc aattgtagcc
     2761 gacatgattc aggggaagat ccagaataaa cttcaggcct ttttggtgcc cgatttgtaa
     2821 ggcggctttt aaatcctcca aactgccata cgccggattt accgcaaaaa aatcattaat
     2881 ggaatagccg tgaaaggact gagattttaa aatcggcaaa aaccagacca ggtcgattcc
     2941 cagatcgacc aagtagtcca ttttttccgc caaaccccta aaatcaccga ttccatcacc
     3001 gttgctgtca taaaatgaac gaaggtaaag ctcataaatg ttcatgaccg actcctgtct
     3061 ttttaataag ctgccgatag ggacgattca ctcttcttgc atccggagct ttcgggctcc
     3121 ggtcgaatat ttatgatatg cttctttgat ttgagtattc ggctggaaat taagttttaa
     3181 accctgatcg tcgatttgat aggagtttcc atagatcatg atcgtgacgg gggtatgatg
     3241 gttggttttt ataaaaatgt gttcttgatt tacagataaa ctcagaatgg cattttgata
     3301 caatatccgg aaactatagg atttccattg cgggggcagt gaaggattaa atgctaacat
     3361 cgggccgtcg gatcgcattc ccccgaagcc atagacaata ttcatccaag cggcggcgat
     3421 cgacgtaata tgcaagcctt cccaggtatt gcggttataa tcatccagat ccagacgggt
     3481 tgcaaaacgg aagaattcgc atgcctcctc aagctctccg atttccgcgg cgatgatgga
     3541 atgaatggat ggggacagtg atgattcatg gatgcatttt ggttcgtaat agtgataatt
     3601 ggcccgtttg gtttgaatgg aaaagtcttg gctatataaa aagagcatca ttaaaacatc
     3661 cggttgtttg atcatatcat ggcgaaagat ccgatcgtat gaccaatggt tatataatgg
     3721 aaaatcatcc gtcgggatgg catcgatatt gagatggggc aaggtaaaaa agccgtcatg
     3781 ctgttcgtat acaccggtgg ctttgttttg tggaagaacc atattggccg taatttgctt
     3841 ccatttttcc gattccatcc cgttgtatcc ggttttttgg atcacggcgg atagagcttc
     3901 aggcgcatcc tttttcatct cgtcaatgac cgcgacggta aattccagtg cctttttggc
     3961 caaatagttg ataaagcaat tgttattgac catcatgtgg aattcgtcca cacccattac
     4021 accgtaaaaa ccatattgta atgtatcggg attctgttgg acgcggctgg cataaaaacg
     4081 gcttatttga atcagcattt ctaccccttg atgataca
//
LOCUS       DASVMB010000294         8474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_478277,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000294 DASVMB010000000
VERSION     DASVMB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478277"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..284)
                     /locus_tag="VF531_08460"
     CDS             complement(63..284)
                     /locus_tag="VF531_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7714042.1"
                     /translation="MPIYEFRCRDCRREFEELCGMGCESLPCPSCHSRNTQRKLSTFA
                     AKSSGSSGSQNLGGHSCGSCHGGSCSGCH"
     gene            complement(307..882)
                     /locus_tag="VF531_08465"
     CDS             complement(307..882)
                     /locus_tag="VF531_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018883310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HEX7714043.1"
                     /translation="MSDLKVSVGISNRHIHLCQADLETLFGKGHQLTPKKDLSQPGQF
                     ASEEMVNLVGPKGTIAKVRVLGPVRPQSQVEISRTDSFGLGIKAPVRESGVIDGTPGI
                     TLEGPAGKIELKEGVIIALRHLHLHTDEAKVMGLKDKDWISVKFEGEREVTFGHVLVR
                     VGPKYAMDLHIDTDEANAAALNNGDQGIVIK"
     gene            complement(1055..1130)
                     /locus_tag="VF531_08470"
     tRNA            complement(1055..1130)
                     /locus_tag="VF531_08470"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1095..1097),aa:Trp,seq:cca)
     gene            complement(1346..1747)
                     /locus_tag="VF531_08475"
     CDS             complement(1346..1747)
                     /locus_tag="VF531_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HEX7714044.1"
                     /translation="MEIDHERANLPIGPILYGCVIAVLAILVFALLFSLMTQFGWTGT
                     VRPYSNNIFLIADYLAIILGSIMAGRKSAATGWMVGIGVGFLCSLALLLLTALMRQPV
                     LWGVFTVKTLISCFIGTFGGIIGVNLAGSRG"
     gene            complement(1801..3105)
                     /locus_tag="VF531_08480"
     CDS             complement(1801..3105)
                     /locus_tag="VF531_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714045.1"
                     /translation="MNHQLQAVTLNNQGQLVIWSQDVNGIWQEGEKLASADAAFTVFQ
                     TADLNHDGIPELIAGTAEPGFIYIYRYSTDHWVLHNYEKYVWKSITNLTSGNFDGNGS
                     NDILVQNQEGSLFLLNSSENSLDLIWKSPIFWRPISSFLVRDIDNDAKDELIVTYRTG
                     GIGILKLINNQIVSIWENYLWGKILDISYGDWDNDLQTEILISTTQKVIYFLGYNGKT
                     YQFENQLSSLNYTVERMEFRIADANSQILAADTAGKLHLLEFHSQTKKWIELMVCQVG
                     RVAQLIPEFGAETPYDVAVWLWGTNRLLSKVQFFKTSLFRFWDGQTAYELNPALIYQD
                     DRFYIAPRAIAALPGLGLKYTQTKTGFQIKAGEDLLELNKKDTTTIKLNGTATPNSGQ
                     AQIIGNDLYLEVTGYQNLLNLSFIVDPELRQVSADIPAPPEA"
     gene            complement(3458..5377)
                     /locus_tag="VF531_08485"
     CDS             complement(3458..5377)
                     /locus_tag="VF531_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX7714046.1"
                     /translation="MGTEKNETEFIRLPRLVSDGMVLQRNTKIKIWGWATADRPVTIQ
                     FLQTMYKTKTDSDGKWSVIIPEQEAGGPYCMEITVATRKITLRDILIGDVWVCAGQSN
                     MQLSMERVKDKYSDIVAKADNNFIRQFVVPMRYNFKSPCDDLESGSWASVNRETILQF
                     TATGYFFAKELYDKYQVPIGLICTAIGGSQVESWLSEESLRHFPAIFKEIAQLRDDQF
                     IEKTQHEEEARDNRWHNILNSRDKGLQNHELPWYDENIDDSNWQIFELPDYWADDDSC
                     RSHGVFWFRKEVTIPVSMVGISARIFMGRIVDNDTVYINGVLIGSTSYQYPPRKYDIP
                     CDVLKEGKNTIAVRVINYRGKGGFIKDKPYQLLAGDQSIDLKGQWKFQIGTVMDMLPQ
                     TTFFQYKPLGLFNGMIAPLFYYSMKGVIWYQGESNTENPDSYKELFTQLIYGWRERWK
                     KDEMPFLFVQLPNYFERENKSAVNKWPLLREAQLKTLEVPHTAMAVAIDLGEWNDLHP
                     LNKQEVGKRLAAAAENLAYGDGDVVPMGPIYQCMEIHDNQIYLNFTNIGSGLYAKGGE
                     NLNNFEISGADGRCVSANARIEGDRVRVWSDFVKSPTAVRYAWKDNPENINFYNKEGL
                     PATPFRTDPYESRVL"
     gene            complement(5586..5662)
                     /locus_tag="VF531_08490"
     tRNA            complement(5586..5662)
                     /locus_tag="VF531_08490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5626..5628),aa:Pro,seq:ggg)
     gene            complement(5993..7075)
                     /locus_tag="VF531_08495"
     CDS             complement(5993..7075)
                     /locus_tag="VF531_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714047.1"
                     /translation="MSKDALAVVEPQTSLQRSKPRYTEAENNILFDSWWNPQKRRELV
                     GHLGRKMSALRSQFCRLLKEKGMTNQDYYNMMQAKYAGTNGTVTRKRSRELKPIDRTI
                     LEIFCKHQALGNSRAQACDELQQILGSLFTSAALKLRFYRLIKRLQYSDQDLLNIGKQ
                     LLGEGRNDSIEFTSPPQKEIAQISSVTVNTTKMETVSKGTYTERGPAQPVMEKQIGSE
                     EPSQEISFLYQLSSLPETIKRLEERVIWIEENQRHQLDLRGFIEHLLAVERDIKREDK
                     LMEEIQRLMDENQIIQNTAEKERERLKRREEELSEVYRMLETMLSDFMRLESVSKLAS
                     LGDFMHRLEITVDQFGNVLKSKRVTA"
     gene            complement(7793..>8474)
                     /locus_tag="VF531_08500"
     CDS             complement(7793..>8474)
                     /locus_tag="VF531_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HEX7714048.1"
                     /translation="DLWRGRIVIGESDLWVLANTDCRMAITTRLKILRRQLKEYISRY
                     PDFQTNLTSYSMDPTAPEIIQWMIRVTAPLNIGPMASVAGAVAGMLGLELMTGLPELI
                     IENGGDIYLRSQKERVVAVYAGSSPFSKRIGLRIAPQPSGIGICTSAGTVGPSLSLGK
                     SDAVVIIASDTALADAAATAVGNRIQTTTDLPAALAYARTISGLSGALLIKEDKLAVR
                     GEVELTPV"
BASE COUNT         2176 a         2076 c         1805 g         2417 t
ORIGIN      
        1 taaaaacaaa aaccgccccg gtttatttgc gggacggttc tttcgcttaa gtttattttt
       61 tgtcaatgac acccgctgca actgccacca tgacaggaac cgcaggaatg tccgcctaaa
      121 ttttgagatc ctgagctgcc gctactctta gcggcaaaag tcgaaagttt gcgctgggta
      181 ttccgggaat gacatgacgg acagggtaag gattcgcaac ccatcccgca caactcttca
      241 aattccctcc ggcaatccct gcacctaaat tcatagatcg gcatgtccaa taccaccttt
      301 atttgcctac ttaatgacga ttccctgatc cccgttgttt aatgcggctg cattcgcctc
      361 atcggtatcg atatgcaagt ccatggcata tttcggaccg accctgacta aaacatggcc
      421 gaaggtcact tcccgttcgc cttcaaactt aacggagatc cagtccttat ccttcagccc
      481 catcaccttg gcctcgtcgg tatggagatg cagatggcgt agggcaatga ttactccttc
      541 ttttaattcg atcttgcccg caggtccctc aagggtgatt cccggagttc cgtcaatcac
      601 accggattcc cggaccggag cctttatccc cagaccgaaa ctatcggtcc gcgagatctc
      661 cacctggctt tggggacgaa ccgggcctaa aacgcgaacc ttggcgatgg ttcctttggg
      721 acccactagg ttgaccatct cctccgacgc aaattggccg ggttgagaga gatctttttt
      781 gggagtcagt tgatgtccct tgccgaacaa cgtctccaga tccgcctgac agagatggat
      841 gtgacggttg gagattccta cggatacctt tagatcggac atgagaaaac gcctccgtta
      901 ttatgtcttt actagtttaa actagtttaa agtctatgtc gtgcgtacgt gtgcagaata
      961 accgattttc aagttccgtt tgattgagga cgaatacaca taaaaaaagc cggttttttt
     1021 aaccgaactt ttcttgcaga ttcttgcaaa ttcatggcag gggagacagg aatcgaaccc
     1081 gcagccaccg gttttggaga ccgacgctct accaattgag ctactcccct gtgcacctat
     1141 caattatagc caataaactt ggttttgtca atttggattt tttgggtgat ctttcgactc
     1201 tgttcttcta aacggttgaa cctggaatca aggataagtg aataatatct cgtgtccaaa
     1261 ggatttgaca ctgtcaaggc ggagaaaaat ccgaatcaag aagtgcgaag taaaaatctt
     1321 ttacttcact cttcaccctt tagaattatc cccgggagcc tgccaggttt accccgatga
     1381 tgcccccgaa agtgccaata aagcaactga ttaatgtttt gaccgtaaat acgccccata
     1441 aaaccggttg ccgcatcaaa gcagtcaata atagcagcgc taaagaacag aggaaaccga
     1501 ctcctatccc gaccatccac ccggtggccg cgctttttcg cccggccatg atggatccga
     1561 gaataatggc taaataatcg gcgatcaaaa agatgttgtt gctatatggt cgaacggttc
     1621 cggtccagcc gaactgggtc atcaaggaga ataatagggc aaataccaat atggccaata
     1681 cagcgatgac gcaaccatat agaatcgggc cgatcggaag attagccctc tcatgatcga
     1741 tttccactat taacctcacc ccttcagttt ttactacaac ttatggggcg atccgttaaa
     1801 ttatgcctca ggcggagccg ggatatcggc actcacctgc ctaagctccg gatctacgat
     1861 gaaggacagg ttcaataggt tttgatagcc ggtaacctcg aggtagagat cattaccgat
     1921 gatttgggcc tggccactgt tgggagtagc ggttccatta agctttatcg ttgtcgtgtc
     1981 ttttttattc agctccaata gatcttctcc agcttttatc tgaaatccgg tcttggtctg
     2041 ggtgtatttc agacccaatc ccggcagggc ggcgatagca cgaggcgcga tataaaaacg
     2101 gtcatcttgg tagataaggg cgggattcaa ttcataggcg gtctgtccgt cccaaaaacg
     2161 aaatagcgag gttttgaaga actgaacctt actcaataac cggttggtgc cccacaacca
     2221 caccgcaaca tcatatgggg tctccgctcc aaactccggg atgagttgtg ctaccctgcc
     2281 aacctgacat accataagtt ctatccattt ttttgtttgg gagtgaaact caagcaggtg
     2341 gagcttgcct gcagtatcgg cggccaggat ttgggagttg gcgtcggcaa tccggaactc
     2401 cattctttcc acggtatagt tcagagacga cagctggttt tcaaattgat acgtcttccc
     2461 gttatacccc aaaaaataaa tgaccttttg cgtggtgctg atcaggatct cggtttgaag
     2521 gtcattatcc caatcaccgt acgaaatgtc gaggatctta ccccataagt aattctccca
     2581 gatcgagacg atctggttat tgattaattt aagaattcca atgcccccgg tccggtaagt
     2641 gacgatcaat tcatctttgg catcgttatc gatatcccgt accaaaaacg aactaatcgg
     2701 ccgccaaaag atgggactct tccagatcag gtccagggaa ttttcggagg aattcaaaag
     2761 aaagagcgat ccttcttggt tttgaaccag aatatcattg gagccgtttc catcgaaatt
     2821 tccggacgtt agattcgtaa tcgacttcca gacatacttt tcatagttgt gcaaaaccca
     2881 gtggtcggta ctataacggt agatatagat gaatccaggt tcagccgtac cggcgatcaa
     2941 ctcgggaata ccgtcatgat tgagatccgc cgtttgaaag acggtaaatg cggcatcggc
     3001 cgacgctaac ttctcacctt cctgccagat accgttcacg tcttgcgacc agattaccag
     3061 ttgaccttgg ttgtttagtg taactgcctg caattgatgg ttcaatgtaa tccaccgggt
     3121 cttcgcagga tccaaatcaa gtagagtgcc ggttcctggt ttcccgtacc aacttccgta
     3181 cagcggggca ggcggcaatg ccggagcagc cagggttgtt gggaaaatcc atgtggaaaa
     3241 aagaaggacc catattgcag agcccactac ggatttgaag attctcaatt ccgatcgctc
     3301 cttgtcgatt aggatcgaat gaatcggaca acttagcggt ttcaggcgtt gacttaagtt
     3361 aataagccac tatctaagat cttatttcaa tatttgagag ttaattcctt caaattaatc
     3421 tgaataggtt ctcgcatcat ggcacacttt cttttcatca aagtactctc gattcatatg
     3481 gatcagttcg aaaaggtgta gccggtaacc cttctttatt ataaaaattg atattctcgg
     3541 ggttatcctt ccaagcatat cttactgctg tcggagattt cacaaaatcg ctccatactc
     3601 taacccggtc gccttcaatc ctggcattgg cgctaacaca tcgaccatcc gcacctgaaa
     3661 tctcaaaatt gttaagattc tccccgcctt ttgcatacaa tcccgaaccg atatttgtga
     3721 aatttagata tatttggtta tcatggattt ccatacattg ataaataggt cccatcggta
     3781 caacgtcgcc atcaccatag gcaagatttt ccgccgcggc agccaagcgt ttaccaacct
     3841 cttgtttatt taggggatgt aaatcgttcc attcgcctag atcaatcgcc acagccattg
     3901 cggtatgggg tacttcaagg gtttttaatt gcgcttctcg tagtaaaggc catttattta
     3961 cggctgactt gttctctctc tcaaaatagt tgggaagctg gacaaaaaga aacggcatct
     4021 catccttttt ccatctttct ctccagccat aaatcaattg agtgaacaat tctttataag
     4081 agtccgggtt ttccgtgttg gactcaccct gataccagat tacacccttc atcgagtaat
     4141 aaaaaagcgg cgcgatcatt ccattgaata agcctaaagg cttgtattgg aaaaaggtag
     4201 tctgaggcaa catatccatg accgtaccaa tctgaaattt ccactgtccc ttcaaatcaa
     4261 tactttgatc accggccagc aactgataag gcttatcttt aatgaacccg ccttttcccc
     4321 tgtaattgat tactctgaca gcgatggtat ttttcccttc ctttaaaaca tcacaaggaa
     4381 tatcatactt tctcggcggg tattgatagg atgttgaccc gattaaaaca ccgttgatat
     4441 aaacagtatc gttatcgacg atgcgtccca tgaatattct tgccgatatc ccaaccatgg
     4501 aaactggaat ggttacttcc tttcggaacc aaaatactcc atgcgaacgg catgagtcat
     4561 catctgccca atagtccggt aactcgaaaa tttgccaatt tgaatcatcg atgttctcat
     4621 cataccaagg caattcatgg ttttgcaaac ctttatcacg gctgtttaaa atattgtgcc
     4681 agcgattatc acgggcctct tcttcatgtt gggtcttttc tataaactga tcatcacgta
     4741 attgtgctat ttctttaaaa attgcaggaa aatgccttaa tgactcttca cttaaccagg
     4801 attcaacctg tgaaccacca atggcggtac agattaaacc aattggaact tgatatttgt
     4861 cgtacaattc cttggcaaag aaatatccgg tggcggtaaa ctgtaatatg gtttctcgat
     4921 ttacagatgc ccaactgcct gattcaaggt catcacatgg cgatttaaaa ttgtaccgca
     4981 tcggaacaac aaattgtctg ataaagttat tgtccgcttt tgccactata tcgctgtatt
     5041 tatccttcac tcgctccatg gataactgca tattggattg tccggcacaa acccaaacat
     5101 ccccaattaa gatatcacgt aaggtaatct tccgggtggc aacggttatt tccatacaat
     5161 aagggccacc ggcttcctgt tccggtatga ttaccgacca cttgccgtcg gaatcagtct
     5221 tggtcttgta catcgtctgt aaaaactgta tggtaaccgg tcgatccgct gttgcccatc
     5281 cccatatttt aattttggta ttccgttgta aaaccattcc atcactgact aagcgaggca
     5341 ggcgaataaa ttccgtttca tttttttcag tacccatgat cacacccgct tatatattcc
     5401 gatttaactt tcaatatcga cattactatt ggtttttact acactttact aaaaaagttc
     5461 tccaaagtta ttgtcatttc ctttctactg gagaactttt cctgcttaac cttgaaactc
     5521 attcactgat ataaaacaaa aaaattccgg gggagattaa accctcggaa tttgaatgtt
     5581 ttctatggtc gggcaggcgg gatttgaacc cacggccccc accaccccaa ggtggtgcgc
     5641 taccaagctg cgctactgcc cggaacataa caaaagatat tttatcacgg atacgtcagc
     5701 cagtcaatag cccaattctg gcagattgaa taaaacttca tcctttatca caatgtattt
     5761 tcctatccgt acaatcattt gcatgcttcc tttttttatt ccgaatccga accggtaagg
     5821 caaacggaat aatcaagaca gggtgattcc gtattccatt tggactctgg aaaggcccaa
     5881 atctgacaaa aacgtcgttc cgtcagatga atacagcccc ggcaagcgtc cggggctgtc
     5941 aaattcagtc ctggcttggt aaggaccatt acattcttga aattggaatc catcacgccg
     6001 taacccgttt gctttttaaa acatttccga actgatctac ggtgatctcc aggcgatgca
     6061 tgaaatcccc aagtgaggcc agtttagaca cactttcaag ccgcataaaa tcggagagca
     6121 tggtttccag catccgatat acctcggaca actcctcttc ccgcctcttc aaccgttctc
     6181 gttccttttc cgcggtattc tggatgattt ggttctcgtc catcagccgt tggatctctt
     6241 ccattagttt atcttcccgc ttgatatctc tttccaccgc caacagatgc tcgataaaac
     6301 cccttagatc cagttgatgc cgttggtttt cctcgatcca gatcacccgt tcctcaagcc
     6361 gttttatcgt ttccggtaat gaagagagtt gatataagaa ggatatttcc tgtgatggtt
     6421 cctccgaacc aatttgtttt tccatgaccg gttgagccgg gccccgctcg gtgtaggtgc
     6481 ctttggaaac ggtctccatt tttgtggtat ttacggtgac cgatgatatt tgggctatct
     6541 ccttttgcgg cgggcttgta aattcgatac tatcattacg gccctcacct aagagttgtt
     6601 tcccgatatt taatagatct tgatcactgt attgcaatcg cttaatcaga cggtagaaac
     6661 gcaactttaa agcagccgat gtaaaaagtg agcctaagat ctgttgcagt tcatcacagg
     6721 cttgggcgcg ggaatttcct aaagcctgat gcttgcaaaa tatctccaga attgtgcgat
     6781 caatcggttt gagctcacgg ctgcgcttcc gggtgaccgt tccgttcgtt cccgcatatt
     6841 tcgcttgcat catattataa tagtcttgat tggtcattcc cttttccttc aataacctgc
     6901 aaaattgact ccgtaacgca ctcatcttac ggcctagatg acctacgagt tcgcgtcttt
     6961 tctggggatt ccaccaggag tcaaataaga tattattctc tgcctccgta taacgcggtt
     7021 ttgatctttg taatgacgtt tggggttcca caactgccaa tgcatctttt gacatattct
     7081 tttcttccct cctatatttt ctagcgcgta tgcgcttcat cgacttccgg ccagataaat
     7141 tagaaagtcg attcaatttg aggcgccttt tgatcaccta agcatgtgcc ggattgcctt
     7201 ttgcataagc tattcgaaag ctgcaatgtt taaaataacg ccggttcgaa atgttatcct
     7261 tccacagttg ctgcttccct tgtaccgtag cttctccgat tcataactcc ttcaagctgg
     7321 ggtttcttca ataagtatct tccaaaagcg ttacccacct gaagttagct taggttgcat
     7381 caaggttcta tcatattttg ttccaggagg aatttaatta tgctaaaatt tttatagatt
     7441 tttgcagcgc ttttgagatt tcaggccaga tcggacggga gtttatgcta cgtttgtgtt
     7501 tgtgtgccgg acataataca acgcctttca gcagaatatc ctgttaagat attcctgaaa
     7561 ggcgttatcg tttgcttttt ttaaaaataa tgattcatta gggattacta atataaaagg
     7621 cgtgaggcat gaatagagca ccccgtgggg cgtcgtgccc attgagcgcg cggcaatagg
     7681 cggaatgagg tgtgagctgc cacccctgtc tgaaagggaa ttcgccaaac ctttgcgggt
     7741 ttgatacttg caacgtccgg ccgtttccct ttacaatcct catgcctcgc gcctataccg
     7801 gcgttagttc tacctcgccc cgaacagcca gtttatcctc cttgattaat aatgcaccac
     7861 tgagaccgga tatggttcgg gcataggcca gagcagccgg aagatcggtt gtggtctgga
     7921 ttcggttccc tacagctgtg gccgctgcat cggcgagggc tgtatcggag gcaatgatta
     7981 caacggcatc ggactttccc aggctaagcg aagggccaac tgtgccggcg gaggtacaaa
     8041 tgcctatacc tgaaggttgt ggagcaattc gtaatccaat ccgtttggaa aaaggcgaac
     8101 ttccggcata aacagccacc acccgctcct tttgtgagcg gagatagatg tcgccgccat
     8161 tttcgatgat cagttccgga agtcccgtca taagctccag acctaacatc cccgcgacag
     8221 cgccggccac ggaggccata ggacctatgt tcaaaggggc tgtaacccgg atcatccatt
     8281 ggatgatctc gggtgcggtt ggatccatgg aatacgaggt taggtttgtt tgaaaatcgg
     8341 gatatcggga tatatactct ttcaattggc ggcgcaggat ttttaatcgg gtagtgattg
     8401 ccatacggca atcggtattt gccagaaccc acaagtcact ttccccgatg acgatccggc
     8461 cgcgccacag atcc
//
LOCUS       DASVMB010000295         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_701108,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000295 DASVMB010000000
VERSION     DASVMB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701108"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..996)
                     /locus_tag="VF531_08505"
     CDS             complement(49..996)
                     /locus_tag="VF531_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714049.1"
                     /translation="MPQFNWKRANAPVIPTAPGTWKETYTANPDLIELGNEYLMYYRG
                     TGNGHDRIGVMRIAKSKFDGFTWEDYPDNPILDVGAPGSFDDINLLDPSAAVVPQASG
                     EQRVYLYYSAVAGSSESIGLAISKDGIHFEKYNQNPVLKGRCPEVVYRDGQFYLFYVK
                     ENERGGYNIHAAMSTDGIHFTPTGGAPVLTVGAQGSWESQTVTTPRLFFDRGLYYMVY
                     AGDDRSKDDPHQFGLATSMDLIHWQKYGGNPIFSTADRKHWDNCSIWYGTAAPIDGGY
                     IMWYEACDEYRGEPRFVSAVGAAVLEAPYFFVKPPLAKD"
     gene            complement(1044..1952)
                     /locus_tag="VF531_08510"
     CDS             complement(1044..1952)
                     /locus_tag="VF531_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714050.1"
                     /translation="MTQISSTQDFLNKFGRHLGKTAATKKFWLHVLLYAVLSAGAVLM
                     IVPFLWMVSTAFKTEPEVLQYPPVWLPRHLTFDSFIKVWQVIPLGRFFFNSIFVGVVI
                     TASQVLLDSLAAYGFARRRFPGRDLIFMIFLTTMMVPRQVTMIPLFMMMRSIPFTGGN
                     GWIDSYWGLIVPGLSGAFGVFLLRQFFITIPVELEEAARIDGCSSINIFFRIMMPLAK
                     PALLSVGIFVFLWSWNDFIWPLIITNQVAMRTLPVGLSNLQGQYKISWSLLMAASVLA
                     TLPVVIAYLFFQREFVQGITLTGLKG"
     gene            complement(1987..2886)
                     /locus_tag="VF531_08515"
     CDS             complement(1987..2886)
                     /locus_tag="VF531_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014599396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714051.1"
                     /translation="MAESGTMNVQRRREMREGYLFIAPAFIHLLIFVILPIFAAIYLS
                     FHNWNILNPSRPFVGLENYKRLWSDELFWMSLKTTLYYTVVSVPIGIVLSLALAMAMK
                     APLKGIGIFRTAYFLPVITSMIVVSLVWTWMFDPLSGLLNYYLKMLGLRPQAWLADPF
                     MAMPSIIILSVWKSLGYNMVIFLAGLQAIPDMYYEAARIDGANAWQQFRRITLPLLRP
                     TTFFILITTLISSFQVFDQVYVMTGGGPANLTKVLVYYIFEVAFHYFEMGYASALSFI
                     LFLFTLVLTALQFKLMSKDSYTL"
     gene            complement(3043..>3948)
                     /locus_tag="VF531_08520"
     CDS             complement(3043..>3948)
                     /locus_tag="VF531_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714052.1"
                     /translation="KYYGYPENYSWNVLAYRKDSFEKAGLDPNKPPKTWNEFLEYSKK
                     LTTGGNYGFVVQPYDWWWFAWFWENGGEIFNKNFTKCTLNSPQGVEALQFYVDLYQKH
                     HVVPPAAVGAMPTEGSHFNAETAFINGQVAMLQVGPWFTMNYATQRPDDVKNLMYAPL
                     PVKDGKTIHASTIGGRIVAVSNQSKNKQLAWEFSRFVVETEVKYYQDMLKNQTPEQAV
                     AKIPLFSLLVRRSSAKLAAFQHPLLKVFVDTAPQATLRPRYTNWVKPARTINQELQAA
                     ILGLKSPKEALDNMVTIINAEMKKK"
BASE COUNT         1070 a         1017 c          954 g          907 t
ORIGIN      
        1 gaaatacttg gttccggtat tcatgacaac ccccttgaat ttcgccggtt aatccttggc
       61 aagcggcggt ttgacgaaga aatacggcgc ctcaagcacc gcggctccca ctgcgctcac
      121 aaagcgcggc tcgccccggt attcatcgca ggcctcatac cacatgatat acccgccatc
      181 aattggtgca gccgtaccgt accaaatgct gcagttatcc cagtgcttgc ggtcggcagt
      241 ggaaaagatc ggatttcccc cgtatttttg ccaatgaatc agatccatcg atgtagcgag
      301 gccgaattga tggggatcat ccttggatcg atcatctccg gcatagacca tataatagag
      361 cccgcgatcg aaaaagagcc gaggtgtggt cacagtttga ctctcccaac ttccctgggc
      421 tcctacggtt aacaccggag cgccgccggt aggggtaaaa tgaatcccat cggttgacat
      481 ggcggcatgg atgttatatc cgccccgttc gttctctttg acataaaaaa gatagaactg
      541 accatcacgg tagacaactt cagggcaacg gccttttaag accggatttt gattgtactt
      601 ctcgaaatga attccatcct tggaaatggc cagaccgata ctctcgctgc tcccggcgac
      661 cgccgagtaa taaagatata cccgttgttc cccggaggcc tgcggtacaa cggcagccga
      721 aggatcaagc agattgatgt catcaaaact tccgggtgct ccaacatcca gtatcgggtt
      781 gtccggataa tcctcccagg taaaaccatc aaatttactt ttggcgattc gcatgacccc
      841 gatccggtca tggccgtttc cggtacctct ataatacatc aagtattcat tacccagctc
      901 aatcagatcc gggttggcgg tatatgtctc tttccaggtt cccggggcgg tcggaatcac
      961 cggcgcattg gctctcttcc aattgaattg aggcactttt tcattcctcc ctcatgggat
     1021 gataattcta atgaattttc atattaacct tttaacccgg ttaaggtgat gccctggaca
     1081 aactcccgct ggaagaagag ataggcgatg actaccggga gcgtagccag gaccgaggct
     1141 gccatcaaaa gcgaccagga gatcttatac tgaccctgga ggtttgacag ccctaccggc
     1201 aatgtccgca tcgccacctg gttggtgatg atcagcggcc agataaagtc gttccaactc
     1261 caaagaaaga caaagatccc caccgagagc agggccggct tggccaaagg catcatgatc
     1321 cggaaaaaga tatttatcga gctgcagccg tcaatccggg ccgcctcttc cagctccacc
     1381 ggaatggtga taaaaaactg gcgcaacagg aaaacgccaa atgcaccgct gagaccggga
     1441 acaatcagcc cccagtaact gtcaatccaa ccgttgcccc cggtaaacgg gatggaacgc
     1501 atcatcataa agagcggaat catcgtaacc tgccgcggca ccatcattgt agtcaaaaag
     1561 atcatgaaga tcagatcccg gcccggaaaa cgccgccgtg cgaaaccgta tgcagccagc
     1621 gagtccaaca aaacctggga ggctgtgatt acaactccaa caaaaatgct gttaaaaaag
     1681 aaccggccga gggggatgac ctgccatact ttaataaaag aatcgaaagt caaatgacgc
     1741 ggcaaccaga ccggtggata ctgcaaaacc tcgggttcgg ttttgaaggc ggtcgagacc
     1801 atccagagaa aggggacgat catcaaaacg gcgcccgcgc ttaatacagc atataataac
     1861 acatgcagcc agaatttttt agttgccgcc gttttaccca aatgtcggcc aaacttattt
     1921 aaaaaatctt gggtggaact tatttgggtc attgctatca ctcctattat ttacccgcta
     1981 attgatttac aaagtgtatg aatccttcga catcagcttg aactgcaagg ccgtaaggac
     2041 cagtgtaaag aggaaaagta tgaatgacag cgccgatgcg tagcccatct cgaaataatg
     2101 gaatgcaact tcaaaaatat aataaacgag gactttggtc agattcgccg gtcctccgcc
     2161 cgtcatcaca tatacttgat caaaaacctg gaatgaggag attaatgtgg taatcaagat
     2221 gaaaaaggtt gtaggccgca acagcggcag tgtaatccga cggaactgtt gccaggcgtt
     2281 agccccgtca atccgtgcgg cttcatagta catatccggt attgcctgca atccggctaa
     2341 aaaaatcacc atattatatc ccagtgattt ccaaacactt aaaatgataa tcgagggcat
     2401 ggccataaac ggatcggcaa gccaagcctg aggccgtaac cctaacattt tcagatagta
     2461 attgagtaaa ccggaaaggg gatcaaacat ccacgtccaa accaacgaga caacgatcat
     2521 cgaggttatt acaggcaaaa aataggcggt ccggaatata ccgatccctt ttaggggcgc
     2581 tttcattgcc atcgccaagg ccagtgaaag cacgatgcca atcgggacgc tgaccaccgt
     2641 gtaatacagc gtcgttttta aggacatcca aaaaagttca tcgctccata aacgcttata
     2701 gttttcaaga cccacaaacg ggcggctggg gttgagaatg ttccagttgt gaaaactcaa
     2761 atagatcgcg gcaaagattg gcaggatgac aaagatcaat aagtgtatga aagcaggggc
     2821 aataaacaga tacccttccc gcatctcccg ccgtctctga acgttcatag tgccggattc
     2881 cgccatcaga atttacctcc ttcaaaatca cccgtcgacg aaaaaaactc gtggctttca
     2941 ttgtcgaagc acccactgag cacccactca gttccttgcg atataccggc gcgggggcga
     3001 atactccccc gcgcgtcatg taacgaaatt cgatccaaaa acttactttt tcttcatttc
     3061 cgcattgata atggtcacca tattatccaa ggcttctttc ggggacttga ggcctaagat
     3121 ggccgcttgc aactcctggt tgatcgtacg ggccggtttg acccagttgg tataccgggg
     3181 ccgtaaggtt gcttgaggag cggtatccac aaaaaccttc aacagcggat gttggaaagc
     3241 agcgagcttc gctgaggagc gtcttacgag caggctgaag agcgggattt tggcgactgc
     3301 ctgctcgggg gtctgattct tcagcatatc ctgatagtat ttgacttcgg tctcaacgac
     3361 gaaacgggag aactcccaag ccagctgttt atttttgctc tgattgctga ctgccacgat
     3421 ccggccgcca atggtgctcg catgaatggt cttgccgtcc ttcaccggta gcggggcgta
     3481 catcaggttt ttcacgtcat cgggacgttg agtggcgtaa ttcatggtaa accacgggcc
     3541 cacctgaagc atagccacct ggccgttgat gaaagcggtc tcggcattga aatgggaacc
     3601 ttcggtcggc atggcgccta ccgcagcggg aggaaccacg tgatgtttct gatacagatc
     3661 gacatagaac tggagagctt caacgccctg cggagaattt aaggtgcatt tggtgaaatt
     3721 cttgttgaaa atctcgccgc cattctccca gaaccatgca aaccaccacc agtcataggg
     3781 ctggacaacg aaaccgtagt tgccgccggt ggttaatttc ttggagtact ctaagaactc
     3841 gttccaggtt ttcggcggtt tgttcggatc cagaccggcc ttttcaaagg agtctttccg
     3901 gtaagctaaa acgttccagg agtagttctc gggatatccg taatattt
//
LOCUS       DASVMB010000296         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_37028,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000296 DASVMB010000000
VERSION     DASVMB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37028"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..732
                     /gene="flgB"
                     /locus_tag="VF531_08525"
     CDS             328..732
                     /gene="flgB"
                     /locus_tag="VF531_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX7714053.1"
                     /translation="MWSTLIETPSAKKMERAMDASNLRQQLLSQNLANVNTPYYKRLD
                     IDFSSVFTQEIDKNELQLWQTHSRHLGGVTPVTGSIQVTRETKTLERYDQNNIDPEYE
                     MAQVAENSLFFQSLTTSWNREMSRIKQAIDGR"
     gene            738..1169
                     /gene="flgC"
                     /locus_tag="VF531_08530"
     CDS             738..1169
                     /gene="flgC"
                     /locus_tag="VF531_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX7714054.1"
                     /translation="MDLFRNLDISATALTAERLRMDLISDNIANINTTRTKEGGPYER
                     KVPVFMEVLDEYMGSNGRPVSKPAGVKVLKIQPDGNPPRLVYDPSHPDANADGYVAYP
                     DINPVSEMVNLITASRSYEANVTAFNSSKDIFRAALEIGKI"
     gene            1222..1551
                     /gene="fliE"
                     /locus_tag="VF531_08535"
     CDS             1222..1551
                     /gene="fliE"
                     /locus_tag="VF531_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX7714055.1"
                     /translation="MNLINRPVAPFSILKMQAAQETQTGNKQDEESNALMATSFEQSL
                     AKALEPVNNLQLESADLDEKLATGNLEYVHQAMVVGEKASLALDLTVQIRNKVIEAYQ
                     EIMRTQV"
     gene            1766..>3133
                     /gene="fliF"
                     /locus_tag="VF531_08540"
     CDS             1766..>3133
                     /gene="fliF"
                     /locus_tag="VF531_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX7714056.1"
                     /translation="MDLRAIFEQLKTLWTKMDATRRWITVVAILLVAGLIFLITRIAT
                     TPKYELLYSKLTEEDKAQIIVKLNEMKVPYRESASGGIEVPNATTVRANLLTQGIPSG
                     GSVGWEIFDQTSFSDTDFTNQIKKQRAIIGEIERTMRKISGIDDAKVILNMADDSEYI
                     FADDKPEGSASIQLTLHEPGILSENQVESILNLVKNATGLKEDNITIVDNFANDLTAA
                     LRPRQTGSLGGKSGSNGTSLADRFAYTAQYEEETRRNVERLLAKPFGFKKVVAVVKAE
                     LDLDYQETKSQTFADKGVPRSEQEKNETYEGTSANSVGVPGTDSNITQYKAPDAGNVV
                     MKGEKSEHTVNNEISNVEQFTVNAPGKVKRQSVSVYVDGNLTPEIKQSVYNLAKEAAG
                     VVEERGDKLSVETFSLAPAAQPARQTIPWTNWSIAAVLGALLLTLIILVIVKEPAPQR
                     VETVKT"
BASE COUNT          900 a          715 c          788 g          730 t
ORIGIN      
        1 tttaccgaat atttgacggc cgattgggtc tgggaggaga acgtcaacct gaaagatgcg
       61 accggatatt aaaatgaagc gctacgacta tttaaagccg tagcgctttt gaatcctgga
      121 tttaattatt tcctccaagg aggataaaaa aacttacttt ttttgcgaaa atccgcaaaa
      181 atccactaaa ggtgcaggat tttccgggag ttgcagcgaa atatctggaa tgagatcatc
      241 aaaaggccta aactttcgga ttcgttgatc cgatgtatga aataagtgat gttttatcat
      301 caatatttca tcagacgagg tggcataatg tggtccacat taattgagac tccgtccgcg
      361 aaaaagatgg aacgggcaat ggacgcctcg aacttgcgtc aacagctttt atcccaaaat
      421 ttggccaatg tgaacacccc ttattataaa aggttggata tcgactttag ttcggttttt
      481 actcaagaaa tcgacaaaaa cgaactgcaa ctttggcaga cccactccag gcatctgggg
      541 ggagtaactc cggtgacggg gagcattcaa gtcactcggg agacaaaaac tctggaacgt
      601 tatgatcaaa acaacattga tcctgaatac gaaatggccc aggtagccga gaattcatta
      661 ttcttccaat cgcttacgac atcgtggaat cgagagatgt cacggatcaa acaagcgatt
      721 gacgggaggt aacgaccatg gatttattcc gaaacctcga tattagtgcc accgcgctga
      781 ctgcggaacg gttacgcatg gatctgatct ccgataatat cgccaatatc aataccaccc
      841 ggaccaaaga aggcgggccc tacgaacgga aggttcccgt tttcatggag gtgctggacg
      901 aatatatggg ctccaatggc cgacctgtct cgaaaccggc cggggtcaag gttttgaaga
      961 ttcagccgga cggaaatccc ccccggttag tatacgatcc ttctcatccg gatgccaatg
     1021 ctgatggtta tgtggcctac ccggatatca atcctgtaag cgagatggtg aacctcatca
     1081 cagccagccg atcttatgag gcgaacgtta ccgcgtttaa ttcctccaaa gatattttca
     1141 gggctgcgct ggaaatcggt aagatttaaa atgcctttcg gcttgaaagt tccgatctcg
     1201 taaaagatgg agggaagtcg aatgaatctg attaacagac cagttgcacc gttttcaata
     1261 ttaaaaatgc aggctgctca ggagacccaa accgggaata agcaagacga agagtccaat
     1321 gccttaatgg ctacctcttt tgaacaatcc ttggcgaagg ccctggaacc ggttaataat
     1381 ctgcagttgg aatccgctga tcttgatgaa aaattagcca cgggtaatct tgaatacgtt
     1441 catcaggcga tggtggtggg ggaaaaggcc agtttggcct tggatctgac ggttcagata
     1501 cgtaacaagg tcatcgaggc ttaccaggag attatgcgga ctcaagtctg ataaattccc
     1561 gtactgacac ccgcggaagc attccatgag ccaaggattg ggcgagaaaa tcaacaacgg
     1621 attgcagaaa tgctttattt ggcaagatcg ggattttaac aaattaaccg tttccggatt
     1681 cggcccggct ttttagaacg gatttgcttc catgccgggt cgccagccgg ataagtcttc
     1741 agcgcctaac cttggaggat tcaagttgga tctgagagct atttttgaac agcttaaaac
     1801 gctttggacc aaaatggacg ccacccggcg ttggattacg gtggtggcta tcctgcttgt
     1861 ggcggggttg atcttcctga tcacccggat cgccacaacc cccaaatatg aattactcta
     1921 ctccaaatta accgaggaag acaaagccca aatcattgtg aaactgaatg agatgaaggt
     1981 cccctatcgg gagtccgcca gcggcggcat cgaggttccc aatgccacca cggtccgcgc
     2041 caacttactg acacagggaa ttcccagcgg cggtagcgtc ggctgggaga tcttcgatca
     2101 aaccagtttt agtgatacgg atttcaccaa tcagatcaaa aaacaacggg cgatcatcgg
     2161 tgagatcgaa cggaccatga gaaagatatc cggtatcgat gacgccaaag taatcttgaa
     2221 tatggcggat gattccgagt atatcttcgc cgacgataaa ccggaaggaa gcgcctcgat
     2281 tcaactgacc cttcatgaac cgggtatcct tagcgaaaat caggtggaat cgattttaaa
     2341 cctggttaaa aatgccacag gtcttaaaga ggataacatt acgattgtcg acaatttcgc
     2401 caacgatctg accgcggctt tacggccccg tcaaaccggg agcttgggag gaaaatcggg
     2461 cagtaacggt accagtttag cggatcgttt cgcgtatacc gcccaatatg aggaggaaac
     2521 ccgccgcaat gtggaacggc ttcttgccaa gcccttcggt tttaaaaaag ttgtggctgt
     2581 ggtcaaggcg gaattagatc tggattatca ggaaaccaag agtcaaacct ttgccgataa
     2641 aggggttccc cggagcgaac aggagaaaaa tgagacatac gaaggcactt ccgcgaatag
     2701 tgtcggagtc ccgggaactg attccaatat aactcaatat aaagctccgg atgcagggaa
     2761 tgttgtaatg aagggcgaga agagcgaaca taccgtcaac aatgagatca gtaatgtgga
     2821 acagtttaca gttaatgctc ctggtaaagt caaacggcaa tcagtctccg tgtatgtgga
     2881 cggcaacctt actccggaga tcaaacagag cgtatataac ctcgcaaaag aagccgccgg
     2941 tgtcgtggaa gaacggggcg ataaactgtc ggttgaaacc tttagtcttg caccggcagc
     3001 ccaaccggcc cggcaaacca ttccttggac gaattggagc atcgccgcgg tattgggggc
     3061 tttgcttctc accctgatca ttctggtgat cgtgaaggaa cctgcaccgc aacgagtaga
     3121 gactgtcaag act
//
LOCUS       DASVMB010000297         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_701301,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000297 DASVMB010000000
VERSION     DASVMB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701301"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2891)
                     /gene="rpoC"
                     /locus_tag="VF531_08545"
     CDS             complement(<1..2891)
                     /gene="rpoC"
                     /locus_tag="VF531_08545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7714057.1"
                     /translation="MLDANNFDALKIGLASPEQIRAWSSGEVKKPETINYRTLKPERE
                     GLFCEKIFGPQRDWECHCGKYKRVRYKGIICDRCGVEVTRSKVRRERLGHIELAAPVS
                     HIWYFKGIPSRMGLLLDMSPRHLEKILYFASYVVIDTGNTPLLKKQLLSENEYRESRE
                     KYGQNFKALMGAEAVKKLLEEIDLEKLSVELKKEVKEVSGQRRIRAIRRLEVVESFRK
                     SNSRPEWMVMDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKLHQCGLPQEMALELFKPFVMKRLVDKGYVHNIKSAKRMVER
                     QKTEVWDVLEEVIHEHPVLLNRAPTLHRLGIQAFEPILVEGKAIQIHPLVCTAYNADF
                     DGDQMAVHVPLSAEAQAEARILMLSAHNILAPSSGRPITTPTQDMVLGCYYLTIIKDN
                     APGEGKAFENSTEVIYAYETGGIDLHAKIKVRMEDQLVETTPGRVIFNDRVKLDLDPD
                     PVANFQISNCVIDRGRLGQLIGQCARTHTVTKTAEILDQIKRLGFRFATQSGTTIAIH
                     DVIVPPQKTEILAEADKHVELVEEQFRRGLITGDERYQRFIDIWTKAKENVSEAMTKG
                     LDKFNPVFMMSISGARGNPAQLAQLAGMRGLMSDPSGRIIDLPIRANFREGLNVLEFF
                     ISTHGARKGLADTALRTADSGYLTRRLVDVAQDVIVREDDCGTTEGIRVGAILDGNEQ
                     IEKLRERIIGRIVAEDICDPKTNELIVGVNQEITEDLAERIEKSGIKEVVIRSVLTCR
                     SRYGVCIKCYGRNLATGRLVDVGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGDDITQ
                     GLPRVEELFEARKPKGQAIISEVAGTCKITEVKGVRRISVVTETGEEKV"
     gene            complement(2950..>3307)
                     /locus_tag="VF531_08550"
     CDS             complement(2950..>3307)
                     /locus_tag="VF531_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7714058.1"
                     /translation="RVKTYEAIVKGENVPEPGVPESFKVLIKELQSLGLDVKVLSEEA
                     KEIQIKEDDDDVKEMAKELGLEYDTPRDTGCNEEIEEGEHEEGGNEAGGESFDEEDDE
                     LFEESFEDDHDASGDV"
BASE COUNT          775 a          963 c          744 g          825 t
ORIGIN      
        1 taaactttct cctcaccagt ttcagttact acgctaatgc gacggactcc tttaacctcg
       61 gtaattttac aggttccagc cacttcactg atgattgcct ggcctttcgg cttacgggct
      121 tcaaacaact cctctacacg gggaagacct tgagtgatat catcaccggc cacaccgccc
      181 gtatggaagg tacgcattgt caattgggtt ccgggttcgc cgatggattg agcggcaatg
      241 ataccaaccg cctctccgac atcgaccagt ctgcccgtag ccagattacg gccataacac
      301 ttgatgcata ctccatagcg actgcggcag gtcaaaaccg agcgaatgac aacttcctta
      361 atgcccgatt tctcaatccg ttcggccaag tcttcggtaa tctcctgatt cactccgaca
      421 atcaactcat tggtcttggg atcgcaaata tcttcagcta cgatccggcc aatgatccgt
      481 tcgcgcaatt tctcaatctg ttcattcccg tcaaggatgg cccctacccg aataccctcg
      541 gtcgttccgc agtcatcttc acggacaata acatcctggg ccacatccac cagacggcgg
      601 gtcagataac ccgaatccgc tgttcgaagt gcggtatcgg ccaaaccttt tctggcaccg
      661 tgagtggaaa taaagaactc caaaacattg agaccctcac ggaaattggc ccgaatcggc
      721 aagtcaatga tccggcccga aggatcgctc atcagtccgc gcataccggc aagctgggcc
      781 aactgggcgg gattaccacg ggctccagag atggacatca tgaaaaccgg attaaattta
      841 tccaaacctt tggtcatggc ctcggagaca ttctccttgg ctttggtcca aatgtcgatg
      901 aaacgttgat accgttcatc accggtgata agacccctgc gaaactgctc ttcgaccagt
      961 tccacgtgtt tatcggcttc ggccaaaatc tccgtttttt gcggaggaac aattacatca
     1021 tgaatagcga tggtggttcc ggattgagtc gcaaaccgga aaccgagccg cttaatctga
     1081 tccaaaatct ccgcggtttt ggttacggta tgggttcggg cgcactgccc aatcaactga
     1141 cctaatctac cccggtcaat tacacaattt gaaatttgga aattggccac cggatcgggg
     1201 tccaaatcca acttgacgcg atcgttgaag ataacccgcc ccggggtggt ttccaccaat
     1261 tggtcttcca ttctcacctt gatcttggcg tgaaggtcga tccccccggt ttcgtaagcg
     1321 tagattacct cggtcgaatt ttcaaaggcc ttgccttctc cgggcgcatt atctttgatg
     1381 atggtcagat aataacatcc taataccata tcctgggtcg gcgtggtaat cggccggccg
     1441 ctggagggcg cgagaatatt atgagccgaa agcatgagaa tacgcgcttc agcttgagcc
     1501 tcagcggata gtggaacgtg taccgccatc tgatcgccgt cgaaatcggc gttatatgcc
     1561 gtacaaacga gcggatgaat ctgaatcgcc ttgccttcga ccaggatggg ttcgaaggct
     1621 tgaataccca aacggtgcaa tgtcggagcg cgattcaaaa gcaccggatg ctcatgaata
     1681 acctcttcca gaacgtccca gacctcggtc ttctgccgtt ccaccatccg tttggcgctc
     1741 ttgatattat ggacataacc cttatccacc aaacgcttca tgacgaaagg cttgaagagc
     1801 tctaaagcca tctcctgggg caaaccgcat tgatgcaatt tcagctccgg cccgaccaca
     1861 ataaccgaac ggccggaata atccacccgt ttccccagta gattctggcg gaaacgccct
     1921 tgctttcctt tgagcatgtc gctaagggat ttcaagggcc ggtttccggg gcccgtaacc
     1981 ggacggccgc gacggccgtt atcgatcagg gcatcgaccg cttcctggag catccgcttt
     2041 tcattgcgga cgatgatgtc cggagcaccc aactccagca atcgcttcaa acggttattc
     2101 cgattgatta cccggcggta tagatcattc agatccgagg tagcgaaccg accgccatcc
     2161 agctggacca taggacggag ttccggggga atgaccggaa taacgtccat caccatccac
     2221 tccgggcgac tgttggattt ccggaatgat tccaccactt ccaagcggcg aatggcccga
     2281 atccttcgct gcccgctgac ttctttgact tccttcttta actccacgga gagcttctcg
     2341 agatcaatct cctcaagcaa ttttttgacc gcttccgcgc ccatcaaggc cttaaagttc
     2401 tggccatact tctcacggga ctcacggtac tcgttttccg agagtaactg tttctttaat
     2461 aacggagtat tgccggtatc aatcacaaca taggaggcga aatacaagat cttctccaga
     2521 tgccgcggcg acatgtcgag cagcaggccc atccggctgg gaattccctt gaaataccaa
     2581 atatgcgata caggagccgc cagttcgata tggcccagac gttcccgccg gaccttggaa
     2641 cgggtaacct ccactccgca acgatcgcag atgattcctt tataacgaac ccgtttatat
     2701 ttcccacaat ggcattccca gtcgcgttgc gggccaaaga tcttctcaca gaacaacccc
     2761 tcgcgttccg gttttaaggt gcggtaatta atggtttccg gctttttaac ctcaccgcta
     2821 gaccaggcac gaatctgttc cggggaagcc aatccaattt ttaaagcatc aaaattattt
     2881 gcatccagca aagaattccc ctccctaagc taaaaaggct ttgccgttcc acttgcagtt
     2941 tccggaaact tagacatccc cggaagcgtc gtgatcatcc tcaaaagatt cctcgaagag
     3001 ttcgtcatcc tcttcatcga agctttctcc tcccgcttcg tttccgccct cttcgtgttc
     3061 tccttcttcg atctcttcat tgcagccggt atcccggggt gtatcgtact ccaaccccag
     3121 ttccttagcc atttctttca catcgtcgtc gtcttccttg atctgaatct ccttcgcttc
     3181 ttcggagaga accttgacat ccaagcccaa gctttgcaat tccttaatca atactttgaa
     3241 tgattcggga acgccgggct ccggaacatt ctcccctttg acgatggcct cgtaagtctt
     3301 gacccgt
//
LOCUS       DASVMB010000298        10554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_552141,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000298 DASVMB010000000
VERSION     DASVMB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552141"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VF531_08555"
     CDS             <1..389
                     /locus_tag="VF531_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7714059.1"
                     /translation="YCLERRDYRLFRLSRIRELVTTPEPFLMQHDNPDRLLAEHGEED
                     RRIFQQVKMVCQTEDRIAMEEYFPNATFTETPGGKWLIECSLPENERGWFGILMSYGA
                     KVRVLEPEELRSRLVQKAREITEMYR"
     gene            504..1760
                     /locus_tag="VF531_08560"
     CDS             504..1760
                     /locus_tag="VF531_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7714060.1"
                     /translation="MNKPSPKEFPHVDWSRNSNIYEVNIRQYTPEGTFRAFEKHLPRL
                     KEMGVDILWLMPIQPIGMKNRKGSLGSYYSIKDYLAVNPEFGTVEDFKRLVDKIHDLK
                     MRVIIDWVANHTAWDHLWATEHPDWYKKNEQGEIHSYIFDHGSEQECWTDVIGLDYSN
                     KELWSAMIEALKYWVREFDIDGYRCDVAGLVPTPFWETARAELEKIKPVFMLAEWSTP
                     ELHEKAFDMTYDWDFYDLMTEFMKGGKNAQDLVKYILKRREIYGEDAYRMMFTTNHDK
                     NSWNGSDTELLGPAFKAFAVLAMTLPGFPLIYGGQESILDKRLAFFEKDPIDWKTYEY
                     TGFYKDLLRMKKLNKALWNGRQGGEISILPAMDEAIFAFTRKKDENQVTVIVNLSDKP
                     RKVAADKASEEFTLEEWGFRIIPGLK"
     gene            1878..2225
                     /locus_tag="VF531_08565"
     CDS             1878..2225
                     /locus_tag="VF531_08565"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7714061.1"
                     /translation="MDNMKRVPTPFSYSAAVGAGDLVFLGLHRGFGDTFPQQLAGTLE
                     SIRQTLGEFGLTLENLVKVNVWLKSIEDLPEMEKLFNHYFEKDHFPARMTSTTEFIDA
                     DCRLMIDGVAYRL"
     gene            2266..2970
                     /locus_tag="VF531_08570"
     CDS             2266..2970
                     /locus_tag="VF531_08570"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7714062.1"
                     /translation="MKRKTLKKAAELIAGASTAFLTSIGENGYPRTVAMANIKTEGLK
                     AVWFSTGTNSNKVRHYRANPKASVCYAIDGHNITLTGDIRVIDDMETKQRLWVDWFIE
                     HFPGGVTDPDYCILKFETKWISLWLDDDFGEISISDAEARIQTLAESYCGLLCSECDF
                     RERCNCGGCIATQGRPYHGECRLAVCCQSKGLTHCGECSDFPCALLNEFAYDQEHGDN
                     GKRIEQLKKWKDCDLN"
     gene            complement(3112..3984)
                     /locus_tag="VF531_08575"
     CDS             complement(3112..3984)
                     /locus_tag="VF531_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7714063.1"
                     /translation="MEHPVETSPNKRGYLLEDFLFFHLRDSQTEAYEYHYHDFYKVII
                     FIAGKVTYLVEGSTYPLKPWDILLITDRDIHKAVIDSNTTYERMVFWFKPGMLAKASS
                     TGSNLLTCFDTASREKRHLLRLDPVVLNELRNMLTGFEAAHDSNLFGSDIMKHSLFLT
                     LIVHLNRAFLQTEPFSRPIQAACDETIRSVIQYIDDHIGGDLSIVNLATKFYLSKYTL
                     MHKFKEQTGYSVHSFILQKRLLVAGSYIKKGLPVMEASFTCGFTDYSSFVRAFKKFYG
                     VSPRQYAKANHPVS"
     gene            4137..5606
                     /locus_tag="VF531_08580"
     CDS             4137..5606
                     /locus_tag="VF531_08580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7714064.1"
                     /translation="MVLKELLAGTKYKVIQGTDGIPIDFVAWDSRKIKPHSLFICIKG
                     LHADGHDFAWKAVADGALALVAEREVGGIAPYTSLIKVDNSRMAMASIANLYYGEPSK
                     RFNLIGITGTNGKTSVSWFIARILENAGRKAGIIGTIENRVGDKLMKVEKLNPTTPDA
                     LELQATFREMLDEGVTDVAMEVTSAALVSHRVDHCHFKVGVFTNLSQDHLDLHGSMEN
                     YKQAKMRLFKMCRYGVLNADDPAHLDIWKHATCDRVMTYGIHKAADLRGENIRYDLDG
                     VSFTLNFYGIKQEVRIKVPGRFSVYNALAAMAACYLAGGLTLEQALEGVQKIEGVRGR
                     FESVPNRQGYRIVVDYAHTPDGLDNILRTARGMTKGRVIAVFGCGGDRDRAKRPLMGE
                     IAGRWADFCIITSDNPRNEEPQQIMDDIETGLQKTGCMYEKIEDRRTAIFKAIEAAKP
                     GDIVVIAGKGHETYQVFAKETVHFDDREVVEEYFRDYCN"
     gene            5760..6524
                     /locus_tag="VF531_08585"
     CDS             5760..6524
                     /locus_tag="VF531_08585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714065.1"
                     /translation="MNKAELIGLAKQQLAIDLGCSPELFDKTENSVVHSTLLEGRRRF
                     SGEPDFLRMATFGKGAVIAADERISGWCQEFLKDREGNRVLEYDVMVQIERELQKYHK
                     HLSGVNEGYLPLPGFNRLKDLQSIPVTVKWYEKDEIPSLYQDKRFKNALLYDVNGLRP
                     DVLVVTADIDNQTVAMAGASMDSELFWQIGIDVMPDHRRMGLAVYLVSALTEEILKRG
                     FIPYYGTWSANIASRNVAAGSGYFPAWVETTAKDSK"
     gene            7301..7996
                     /locus_tag="VF531_08590"
     CDS             7301..7996
                     /locus_tag="VF531_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7714066.1"
                     /translation="MGDAEEHLFTIGELAKLVGVSVRTLQYYDQINLLNSTYTDSHKR
                     VYTRDDLLKLLQILFLKSSGIPLQEISNQILKPENSADLIEIFARQREILLNQIANLN
                     KIVNILDLAISEGGQEIGMDRLIAIAEFMKQGKPISDKLRELDERGIDPSGKEGQELA
                     KQWWSIIGEFAGGDVNLLKSLAIAGRDIENWPDEARYQKESAERFLAEALRIYIHNND
                     IQTDMEKRMEPRS"
     gene            8050..9990
                     /locus_tag="VF531_08595"
     CDS             8050..9990
                     /locus_tag="VF531_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7714067.1"
                     /translation="MNPKYKKLFERSYIGKMPLSNKFVMCSMSTHCWDEGGLVSDDQI
                     DYFEERARGGVGMIVVEGQFISSKVDKSLATMTAAGTQVQAARWFMMVERLRGFNTRL
                     CNQLVIGPGRNGGLAIDNALPAPSVVPLFIDPTKKTHAMTKEEIQEVIRAYVKAVVNS
                     KFAGFDCVEIHAHGGYLLDSFMMKLWNHRTDEYGGSIENMARLPVEIVKAVKEAVGPD
                     YPVIFRMPAQHHFEGGRTLEESLGIIKLLDAAGVDAFDIDSGSYETQDWMIPPAYYGD
                     APSLDDAVAVKKVTRKPILITNNITLDIAAEALEKGESDFFMLGRPLIADPHMVRKVF
                     EGKEEDIRPCLRCNEYCVGGNFKGRRTSCSVNPQVLQEKAFKLEKTANPQKVVVIGGG
                     PGGLEAARVAATKGHDVTLYEKAGTLGGQVVAAATPPFKGQLNKYLHYLIGQNEKLGV
                     KIHLNTEINESSPELANADKIIVAIGAKPFIPPIKGINGEKVIEVIEAHLHRHSEIGN
                     KVVIAGGGLSGCDYALELAMEGKEVSIIEILDEVAVNAMIINRIALLKKLADFKVKLL
                     TGKKILEFTNDGVIVESKDGSEQEIKADTVIVSFGTRPLNNLADKIGNKYPSAKAIGD
                     CVSIGQVGEAVRSGFYAAWAID"
     gene            10057..>10554
                     /locus_tag="VF531_08600"
     CDS             10057..>10554
                     /locus_tag="VF531_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7714068.1"
                     /translation="MTDIKGRTAFITGGGNGIGLGIARSLARAGAKLALADLDAAALA
                     RAKAELQDITPVETYVLDVRDRDAYASTAEAVERALGPVSLLFNNAGVAGGDSVRRLT
                     YELWDFYLGVNLYGVINGLQAFLPKMIERGQGGHVVNTASGAGLVASGAGILYTTSKY
                     AVVGMS"
BASE COUNT         2897 a         2245 c         2790 g         2622 t
ORIGIN      
        1 gctattgtct ggaacggcgg gattaccgcc tgtttcgcct ttcccgtatc cgggagcttg
       61 tgaccactcc agagcctttc ttgatgcaac acgacaatcc cgataggctt ctggccgagc
      121 atggcgagga ggaccgtcgt atcttccaac aggtgaagat ggtatgccaa acggaggacc
      181 gtatcgcgat ggaggagtat ttccccaatg cgacgtttac ggagaccccg ggggggaaat
      241 ggttaattga atgcagtctg cctgagaacg aacggggctg gttcggtata ctgatgagtt
      301 atggtgccaa ggtcagggtg ttagagccgg aagaactgcg gtcacggctt gtccaaaagg
      361 ctcgtgagat tacggaaatg tatcggtaat tacgacatat aggcatttct attttttacc
      421 gatccgaacc ggggaatcct tgcggaggat ttatgtaacc aaagtagaag taaagcacaa
      481 aaattcgatc agcgaggtat gagatgaaca aaccatcccc aaaggaattc ccccatgttg
      541 actggtccag aaattccaac atttacgaag tgaatatccg tcaatataca ccggaaggaa
      601 ccttccgcgc ctttgaaaaa catttgccca gactgaagga gatgggagtc gatattctgt
      661 ggctcatgcc gattcaaccc atcggcatga aaaatcgcaa gggcagtctg ggaagctact
      721 attcgatcaa ggactatctg gctgtgaacc cggagttcgg aacagtcgaa gattttaaac
      781 ggctggtcga caagattcat gatttaaaaa tgcgcgtgat catcgattgg gtcgccaacc
      841 atacggcgtg ggatcacctt tgggcgaccg aacatcccga ttggtacaag aagaatgagc
      901 aaggcgagat ccattcctat atcttcgatc acggcagcga gcaggagtgt tggaccgatg
      961 tgatcggtct cgattatagc aataaagagt tatggtcggc gatgatcgaa gctttaaaat
     1021 actgggtccg ggagtttgat atcgacggtt accgctgcga tgtggcggga cttgtgccca
     1081 cgcctttttg ggaaacggcc cgggcggagc tggaaaagat taaacccgtc ttcatgctgg
     1141 cggaatggtc caccccggaa ctgcatgaga aggcctttga catgacgtat gattgggatt
     1201 tttacgatct gatgaccgag ttcatgaaag gcggcaaaaa cgcccaagat ctcgtgaaat
     1261 atatcctaaa gcgccgggag atatatgggg aagacgccta ccggatgatg ttcaccacca
     1321 atcacgacaa gaatagctgg aacggaagcg acaccgaact tttaggtccg gctttcaagg
     1381 cctttgcggt gctggcgatg acgctgccgg gatttccgct catctatggc ggccaggagt
     1441 caatcttaga taaacgcctc gccttctttg aaaaagaccc gattgattgg aagacttatg
     1501 aatataccgg gttttataag gacctgctcc ggatgaagaa gttgaacaaa gcgctctgga
     1561 acggccggca agggggagaa atctccatcc tgccggcgat ggatgaggcg atctttgcct
     1621 tcacccggaa aaaggatgaa aaccaggtca cggtgatcgt gaacctatcc gataaaccgc
     1681 gaaaggtagc ggcggacaag gcatctgaag agtttacgct ggaagaatgg ggtttccgaa
     1741 tcattcccgg tttaaagtaa ccttaggttg tttgcgactg taaaattgtg agagcaatca
     1801 cccaaaccac gacatacagt tgtccgggat ccccgcgtat aatgaaacta tcaaatatta
     1861 cgcgaaagga atgaaccatg gataatatga aacgggttcc aaccccattc agttattcgg
     1921 cggcggtcgg cgcgggggat ttggtgtttt tgggattaca tcgcggtttt ggggatacat
     1981 tcccccaaca actggcggga acactggagt ccatccgcca gactttggga gaattcggac
     2041 tgactttgga gaatctggtc aaggtcaacg tctggctgaa aagtatcgag gatctgccgg
     2101 agatggagaa actcttcaac cattacttcg aaaaggatca ttttccggcc cggatgacat
     2161 caaccaccga gttcatcgat gccgactgcc gattgatgat cgacggggtt gcctaccggc
     2221 tttaacgtgg aatgatcgta tattgaatgg ataggagagg atcttatgaa acggaagacg
     2281 ctcaaaaagg cggcagagct tattgccgga gcatccaccg cctttttaac ctcgattggc
     2341 gaaaacgggt acccccgaac cgttgcgatg gcaaacatca aaaccgaagg tctaaaagcg
     2401 gtttggttct cgaccggaac gaactccaat aaagtccggc attaccgggc gaaccccaag
     2461 gccagcgtct gttatgccat cgacgggcat aacatcactt taaccgggga tatacgagtc
     2521 attgacgata tggaaacgaa acaacggctc tgggtggact ggtttattga gcactttccc
     2581 ggcggagtca ccgaccctga ttattgtatt ttgaaattcg aaaccaaatg gatttcactc
     2641 tggctcgatg atgatttcgg ggaaatcagc atatccgatg cggaagcccg gatccaaacc
     2701 ctggcggaat cctactgtgg gcttttgtgc agcgaatgtg atttccggga gaggtgtaac
     2761 tgcggcggtt gcatcgcgac gcagggtcga ccgtaccatg gtgaatgccg gctggcagtc
     2821 tgctgccaaa gtaaaggttt gacgcattgc ggtgaatgtt cggattttcc atgcgcgttg
     2881 ctaaatgaat tcgcttacga ccaagagcat ggggataatg ggaagcggat tgagcagctg
     2941 aaaaagtgga aggactgtga cttgaattaa atgttcaaag ccacatttgg gagtgataga
     3001 aattgaaata cgtatgtgta aagtcagtcc tgtaaccgcc caattaacag tgagaaaaca
     3061 tctgcaatgc cggggcattg cagatgtttt tttgttctca taggccacct tttaactgac
     3121 cggatggtta gccttcgcat attgcctggg tgaaactccg tagaacttct taaacgctct
     3181 gacaaagctc gaataatcgg tgaatccaca ggtgaaactg gcctccatta ccggaagtcc
     3241 tttcttgata tatgagcccg ccaccagcaa ccgtttttgc aaaataaaac tatgaactga
     3301 atagccggtt tgttctttga atttgtgcat caatgtgtat ttacttaagt aaaacttggt
     3361 tgccaggttt acaatggaca gatccccgcc gatatgatcg tcgatatatt gaatcacgct
     3421 acggatggtt tcatcacagg cagcttgaat cggccgggaa aagggttccg tttgaagaaa
     3481 cgccctattt aagtgaacaa tcaatgtcaa aaacagggaa tgtttcataa tgtcggaacc
     3541 aaataaattg ctatcatgag cggcttcaaa tccagtcagc atatttctca attcatttag
     3601 cacaacggga tccaatcgaa gcaggtgccg tttctcccgg gaagcggtat cgaaacatgt
     3661 caagaggttg ctcccggtac tgctggcttt ggccagcatt ccgggtttga accagaaaac
     3721 catgcgttca tatgtcgtat tactgtcaat gacagcctta tggatgtccc ggtcggttat
     3781 gagcaggata tcccagggtt ttaggggata ggtactgcct tccaccaggt atgtcacctt
     3841 tccggcaata aagataatga ccttataaaa atcgtgatag tgatattcat atgcttccgt
     3901 ttgggagtcc cgtaaatgaa agaagaggaa gtcctcaagc agataccccc gcttattggg
     3961 ggaagtttcg accgggtgtt ccatgggtca actccatttg tcattattta ctctccattg
     4021 tattgcagct tttgcaatat ataaagcact tttggcaaat tattttgcaa aaattttaag
     4081 tatactatta ctgggtaaac atttccgtat aacaatttct gaatgggggc ttttgaatgg
     4141 ttctcaaaga actgcttgcc ggcaccaaat ataaagtaat tcaaggaacg gatgggatcc
     4201 ccattgattt cgtggcttgg gattccagga agatcaaacc tcattcgttg tttatatgta
     4261 taaagggttt gcatgcggat gggcatgatt ttgcctggaa agccgttgcc gacggggctt
     4321 tggcattggt ggccgagcgt gaagtcggcg gtattgctcc ctataccagt ctcatcaaag
     4381 tggataattc caggatggcg atggccagta ttgccaacct ctattacggg gagccttcaa
     4441 aacggtttaa tctgatcggg attaccggca ccaatgggaa aacctcggta tcctggttta
     4501 ttgcgagaat cctggaaaat gcgggacgaa aagcgggtat catcgggacc attgaaaacc
     4561 gcgtaggcga taagttgatg aaggttgaga aactgaaccc gacgacaccg gatgcccttg
     4621 aactgcaggc aacatttagg gaaatgctgg atgaaggggt tacggatgtc gccatggaag
     4681 tgacctcggc tgccctggtg agtcacaggg tggatcattg tcattttaag gttggtgtat
     4741 tcacgaatct atcccaagac catcttgatc ttcatggttc gatggagaat tataaacagg
     4801 ccaagatgag gctgtttaag atgtgccgtt atggtgtgct caacgccgac gatccggcac
     4861 atctggatat atggaaacat gctacctgcg acagagttat gacctatggg atacataaag
     4921 ctgccgatct acgaggagaa aacatccggt atgatctaga cggggtcagt tttacgctca
     4981 atttctatgg tatcaagcag gaagtcagga tcaaggttcc cggacggttc agtgtttata
     5041 acgccctggc tgcaatggcc gcctgttatc ttgcgggagg acttaccctg gaacaggccc
     5101 ttgagggggt gcaaaaaatc gaaggggtaa ggggcagatt tgaatcggtt cccaatcgtc
     5161 aaggatatcg catcgtggtt gattatgccc acacacccga tggtctggat aacatcctgc
     5221 gaactgccag ggggatgacc aagggaaggg tgatcgccgt ctttggttgc ggcggcgacc
     5281 gggatcgggc caaaagaccg ctcatgggtg agattgccgg acgatgggcg gatttttgca
     5341 ttattacatc ggataacccc cggaacgaag agccgcagca aattatggat gatattgaaa
     5401 ccggcctaca gaaaaccgga tgtatgtatg aaaagattga ggatcggaga accgcgattt
     5461 ttaaggcgat cgaggctgca aagcccgggg atattgtggt tattgccgga aaagggcatg
     5521 aaacctacca agtgtttgcc aaggagaccg tccattttga tgaccgggaa gtagttgagg
     5581 aatattttcg ggattactgc aattaaatac ataaagggct tagtattaaa aggaataggg
     5641 ccacctgggg agaattatct ctggtaactt ttgtaaggga gaagttaaac gtctttcgag
     5701 ttgtatttca cacttcacac ttcaaacttc acaattttct atccgggtgg tgaaatatag
     5761 tgaataaagc cgaattaatc ggcctggcca aacaacagct tgccatcgat ctgggctgtt
     5821 ctcctgagct gtttgacaag acggagaata gtgttgtcca ttcgacactg cttgaaggca
     5881 ggcgccggtt ttcgggtgaa ccggactttt taaggatggc gacattcgga aaaggggccg
     5941 taatcgccgc cgatgaacgg attagcggct ggtgccagga gtttttgaag gaccgtgaag
     6001 gcaaccgcgt gctggaatat gacgtcatgg ttcagatcga gcgggaattg caaaagtatc
     6061 ataagcatct ttccggtgtg aacgagggtt atctgccgct gcccggtttt aaccgcttaa
     6121 aggatttgca atctattccg gtaacagtaa agtggtatga aaaagatgag ataccctcgc
     6181 tttatcagga caagcgtttt aaaaatgctt tactctatga tgtcaacggc cttcggcctg
     6241 atgtattggt tgttaccgcg gatatagaca accagacggt ggcgatggcc ggagccagca
     6301 tggatagcga gttattttgg cagatcggta ttgatgttat gcccgatcat cgccgaatgg
     6361 gcctggctgt ctatctagta agtgctctga ccgaagagat attgaaacgg gggtttatcc
     6421 cgtattacgg cacctggagc gccaatattg cttcccgaaa tgtcgctgcg ggcagcggat
     6481 attttcccgc gtgggtagaa accaccgcta aagattctaa atagagtttt cgtgatttcc
     6541 gtgtgtttcg tagttcgtga tcctcttcct ccgctaagtt caagcagctc attgaagaaa
     6601 gatacttttt aaccacgaaa cacactaaat acacgaaaat agatcgtagc gacatacgga
     6661 cccacatgcg tactcttgcc acatctcaag cctttgaaag aggcttattt ggtgtgctta
     6721 tccgatggtt gaatagttaa gagtagtcgt gctacaccct catttacgta tgtgccttgc
     6781 gcgtatgcgc gaaggccact gttcgtgacg catcatgtaa aggtttggcc gtgcttttcc
     6841 gcacggcttt ttttgtgcct ttgacatgcg tcgctttaca ctgtggcttg gccaaatatt
     6901 atcgacgcca caatgaaggg cattaacacc tcaacgcaag cgcattgctt gccgctttcg
     6961 tttcacttct atggttgaca aattattcga ctagaggatt aaattcttaa aaaatatttg
     7021 aaaaaagtac ttgctgacga cgtagcgtcg cgtgatacca ttaattatgc aataaagaaa
     7081 agatgcttgg aatgaagaat tgcatcgaaa atggcgtgac tggcacatcg aaaagaaaca
     7141 ttattatcaa gacgcaacat ggagaagtag aagttgccag tacgcatcaa aatcagcccg
     7201 gtgtatgttc agattagatt gaacacatga tgatttcaga tgaagtaaac ttataccggc
     7261 aaaagggccg tatatattaa cctttacgag gcggagcaga atgggagacg ctgaagaaca
     7321 tttgtttaca attggagaat tagcaaaact agtcggagta tctgtgagaa cattgcagta
     7381 ttacgatcag ataaatctgc tgaattcaac ttataccgac agccataaac gggtgtatac
     7441 acgtgacgat ttattgaaac ttctgcagat tttatttctg aaatcatcag gaatcccgct
     7501 tcaggagata agcaatcaaa ttctaaaacc ggaaaattct gctgatttaa tagaaatatt
     7561 cgcccgacag cgcgaaattc tgctgaatca aatcgcgaat ctgaacaaga tcgtcaacat
     7621 attggatctt gcaatttccg agggcgggca ggagatcggt atggatagac ttatagccat
     7681 cgcggagttt atgaagcagg ggaaaccgat atctgacaaa ttgcgggagc ttgacgaaag
     7741 aggcattgat ccctcaggca aagaagggca ggagcttgca aagcaatggt ggagtataat
     7801 tggtgaattt gctggcggag acgttaactt gttgaaatcg ctggcaattg caggccggga
     7861 tatcgaaaac tggccggatg aagccagata ccaaaaagaa tccgctgaga gatttcttgc
     7921 ggaagccctc agaatttata ttcataataa tgatattcaa acagatatgg aaaaacgcat
     7981 ggaaccgagg tcgtaatgtg gtttttggct tccggcggag taaaaaagcg aaagggatgg
     8041 gatatattta tgaacccgaa atataaaaaa ctgtttgaaa gaagttatat cggtaaaatg
     8101 cctttaagca acaaattcgt catgtgttct atgtcgaccc attgctggga tgaaggaggc
     8161 ttagtgtcgg atgaccagat agactatttt gaggaacggg cccgcggcgg agtcggcatg
     8221 attgtcgtgg aagggcaatt catttccagc aaagtcgaca agtctcttgc tacaatgaca
     8281 gctgccggaa cccaggtgca agccgcgaga tggtttatga tggtcgagag gctccgcggc
     8341 tttaatacga ggttgtgcaa ccagcttgtt ataggcccgg gacggaatgg cgggcttgcg
     8401 attgacaatg cccttcccgc gccttctgtg gttcccctgt ttattgatcc gaccaagaaa
     8461 acccatgcga tgaccaaaga agagatccaa gaggtgatcc gggcatatgt aaaagctgtc
     8521 gtgaattcaa aatttgcagg ctttgattgc gttgaaatcc atgcacatgg cggatatctc
     8581 ctcgattctt tcatgatgaa gttatggaac catcgtacgg acgaatacgg cggaagtatt
     8641 gagaacatgg caagactgcc ggttgaaatt gtaaaagctg taaaagaagc cgtagggccg
     8701 gattacccag tcatcttcag gatgcctgcc cagcatcatt ttgaaggagg acgcacttta
     8761 gaggaaagcc taggtatcat caagttattg gatgcagcgg gagttgacgc tttcgatatc
     8821 gactccggat cgtatgaaac ccaggattgg atgatcccgc cggcgtatta cggtgacgca
     8881 ccttctttgg atgatgcagt tgcagtaaaa aaagtgaccc gtaaaccgat ccttattacg
     8941 aataatatca cgctggacat tgcggcagaa gctttggaaa aaggagaatc ggacttcttc
     9001 atgcttggca ggcctttgat tgccgatccg catatggtgc gcaaggtttt cgaaggcaaa
     9061 gaggaagaca ttcgtccgtg cctccgctgc aatgaatatt gcgtcggcgg gaattttaaa
     9121 gggcggcgca cttcgtgctc tgtaaatcct caagtattac aggaaaaggc gtttaaattg
     9181 gaaaaaaccg ctaaccccca aaaagttgtc gttatcggcg gaggacccgg agggcttgaa
     9241 gcggcaaggg ttgcggcaac aaaggggcat gacgtaacgc tttatgaaaa agccggcaca
     9301 ttgggcgggc aagttgtggc ggccgcaacg cctccgttta agggccagct caataaatat
     9361 cttcattatc tcattggcca gaatgaaaag ctgggtgtca aaattcacct caataccgaa
     9421 atcaatgaga gttctcccga acttgccaat gcagataaaa tcatcgtagc catcggcgct
     9481 aaaccattca tacctccgat taaaggaatc aatggggaga aagttatcga ggtcattgag
     9541 gcacatcttc accgccattc tgaaatcggc aataaagttg tgatcgcagg cggcggttta
     9601 tccggctgtg actatgcctt ggaattggct atggaaggga aagaagtttc aatcatcgaa
     9661 atccttgacg aagttgctgt taatgccatg attattaacc gtattgcatt gctcaaaaag
     9721 ctggccgact tcaaagtaaa acttttgacc ggaaaaaaga tacttgaatt cacaaatgac
     9781 ggtgtgatag ttgaaagcaa agatggtagc gagcaggaga ttaaagccga tactgtcatt
     9841 gtatcctttg gtacacgacc gcttaacaat ctggctgata aaatcggcaa taagtatccg
     9901 tctgcaaagg cgattggcga ctgtgtctcc atcgggcaag ttggtgaagc cgtccggtcc
     9961 ggattctacg cggcatgggc aattgattaa tacgattttt ggggtgttaa gataacttta
    10021 tagttatgca aataatattt taaaggagaa gactatatga cggacattaa aggacgcact
    10081 gcattcatca ccggcggtgg caacggcatc ggattgggca tagcccggag ccttgcgcgc
    10141 gccggcgcca agttggccct ggcagattta gacgccgccg ctctcgctcg ggcgaaggca
    10201 gaactgcagg acattactcc agtcgagaca tacgtactcg atgtgcgtga tcgcgatgcc
    10261 tacgcgtcca cggcagaggc ggtggaacgc gcactgggac cggtatcgct cctgttcaac
    10321 aacgctggtg tcgctggcgg tgactctgtg aggagactca cctatgaact ttgggatttc
    10381 tacctcggtg taaatctcta cggcgtcatc aacggtctgc aggcgtttct gccgaagatg
    10441 atcgaacgcg gccagggtgg ccatgtcgtg aatacagcct cgggtgcggg ccttgtggca
    10501 tcaggcgccg ggattttgta cactacatcc aaatacgcgg tggtcggaat gtcg
//
LOCUS       DASVMB010000299         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_404065,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000299 DASVMB010000000
VERSION     DASVMB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404065"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VF531_08605"
     CDS             complement(<1..1080)
                     /locus_tag="VF531_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7714069.1"
                     /translation="MRGHRLSLSSTRLIRSFNAISVLQTLYKEESATRARLTEVTKMS
                     PATITRIITELTEQGVIAEERIGESNGGRRPVIFRLNNDKLYVAGVQILRDRVALAIS
                     DIRGRLVIRKIFQQYSLEPDSLINEIANEFEMMLASSQVDKEYILGVGLAISGIVDSE
                     NGVLLRSVNLGWHEVRVAELVEKALGLQVFVENDANAAALAEIWLGAAKDVSSMMYLK
                     TASGVGAGIIYERRLLTGARGMAGEIGHIPLIPGGHQCRCGQQGCLETYLYLPDVIHR
                     YEQAHEESLETPDQLFNKAASGEPLARTIVEEAVYALATTISFAEVFLDLEMIVIGGV
                     WGGLGDDFMNQIQDRLQIYLERGGLS"
     gene            complement(1236..2351)
                     /locus_tag="VF531_08610"
     CDS             complement(1236..2351)
                     /locus_tag="VF531_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX7714070.1"
                     /translation="MREIRTGRTGRLVSLDALRGFNMFWIIGAAGIANGLSNLKIPGA
                     SLIASQLRHAPWNGFRFNDLIYPMFIFVVGISVSLALGRRREKGEIPRKIVGHIFFRT
                     ALLFGIGLYMSNSGLNWYGWLHNLRWMGVLQRIALCYSCAALMVLYTGPRRQAGTVAG
                     LLILYWLLLRFVPVPGYGAGIWNPPEANFANYFDSRFLPGRLYYHTWDPEGLLSTMPA
                     LATCLLGVLTGHWLRKPDVKPEFKAGALAIAGMILALLGLLWSLDFPLNKKIWTSSYT
                     LLTAGLSAAGMALFYWLIDIRGFQRWAYPFVVIGRNSLFIYVAADFIPFGAAARFLLG
                     IGPDLGNSMVLIRSIATLGLEWLLLWLMYRFRIFVKL"
     gene            complement(2566..>3028)
                     /locus_tag="VF531_08615"
     CDS             complement(2566..>3028)
                     /locus_tag="VF531_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7714071.1"
                     /translation="DFLAKVKEALHKTGLPPDALCLEITETLLMESFQENTQKLEALK
                     KYGVSLSLDDFGTGYSSLNYLKRLPIDHVKIDKSFVDDIIETGNGRMVIGSIITLAHQ
                     LGLKVVAEGVETREQLDCLSESNCDIIQGYLISPPVPAGEAEVLFTKEKLI"
BASE COUNT          803 a          850 c          683 g          692 t
ORIGIN      
        1 gctcaatccg ccgcgttcca gatagatctg aagtctatcc tgtatctgat tcataaagtc
       61 atcgcccaaa ccgccccaaa cgccgccgat aacgatcatt tccaaatcca gaaagacctc
      121 ggcgaaggag atggtggttg ccagggcgta aacagcttct tcaacgatgg tacgggctaa
      181 gggttcgcca cttgcggcct tgttgaataa ctgatccggt gtctctagcg actcctcatg
      241 cgcctgttcg tagcggtgga ttacatccgg gagatacaga taggtttcca ggcagccctg
      301 ttgtccgcag cggcattggt ggcctccggg aattaacgga atatggccaa tctcccccgc
      361 cataccccgc gccccggtaa gcaacctgcg ctcgtagata attccggcgc ccacaccgga
      421 agcagttttc agatacatca tgctcgatac gtctttggct gcccccagcc atatctcggc
      481 taaggcggcg gcattggcgt cattttcgac aaacacttga agcccaaggg ccttttccac
      541 caattccgcg acccgcactt catgccagcc caaattgacg gatcgaagca aaaccccgtt
      601 ctcactgtcg acaattccgg aaatggctag tcccacacct aaaatgtatt ccttgtccac
      661 ctggctgctg gccagcatca tctcaaattc attggctatt tcgttgatga gtgaatcggg
      721 ttccagggaa tattgctgga aaatcttgcg aatcaccaac cggccccgga tatccgaaat
      781 ggccagggca acccggtccc tcaaaatctg aactccggcc acataaagtt tatcgttgtt
      841 aaggcggaag atcaccggcc tccggccgcc attggattcc ccgatccgtt cttcggcgat
      901 cactccctgt tcggttaact cggtaatgat ccgtgtaatg gtggccgggc tcatcttggt
      961 aacctcagtc agccttgccc gggtagcgct ttcttcttta tatagagttt gaagaaccga
     1021 gatggcattg aacgatcgga tcaatcgtgt actgctcaat gataatcgat gaccgcgcat
     1081 tacaatcccc tacttttaat cactatgaaa aaatctaacc ttattgtaac acggttgtaa
     1141 tactccgtca agtgtttagg attatccccg ggccgctttt attccagaaa attccctcac
     1201 ccatcaatta ctatcagatt gaaaaaatgc ccggtttata gcttgacaaa aattcgaaac
     1261 cggtacatca gccataacag caaccattcc aaacccaatg tggcgattga ccggatcaaa
     1321 accatactgt ttcccaaatc cggaccaatt cccaataaga aacgggccgc agctccaaag
     1381 gggatgaagt cggcggctac ataaatgaaa agtgaattcc tgccaatcac tacgaacgga
     1441 taggcccatc tttgaaaacc acgtatatca atcagccagt aaaacaaggc catccccgcg
     1501 gcactaagac ctgccgtcag tagtgtgtat gaactggtcc agatcttttt attgagcgga
     1561 aaatccaggc tccacaaaag acccaggagc gccaggatca taccggctat cgctaaagct
     1621 cccgccttaa actcgggttt gacatccggt tttcgcagcc aatgcccggt caaaactccc
     1681 aaaagacagg ttgccaaggc cggcatggtg ctgagcagtc cctcgggatc ccatgtgtgg
     1741 taatataacc ttcccggaag aaaccggctg tcaaaataat tggcgaaatt ggcctcgggt
     1801 ggattccaaa ttcccgctcc ataacccggt accggaacaa accggagcag cagccaatat
     1861 agaatgagca accccgcgac cgttccggct tggcgtcgcg gacccgtata gagtaccatc
     1921 aaggccgcgc acgaatagca taaagcaatc cgctgcagca cacccatcca gcgcaagtta
     1981 tgaagccaac cgtaccaatt caaaccggag ttactcatat acaggccgat accaaagagt
     2041 agcgcggtac gaaaaaagat atgtcccacg attttacggg gaatctcgcc tttttcacga
     2101 cggcgtccca acgcaagact gacggagatc cccactacga aaatgaacat cggatagatc
     2161 aggtcgttga accggaaacc attccagggg gcgtggcgca actggctcgc aatcaacgag
     2221 gccccgggga tctttaaatt gcttaaaccg tttgcgatgc ccgcggctcc gatgatccag
     2281 aacatgttga aaccccgtaa cgcatccagc gaaaccaacc ttcccgtccg gccggttcgg
     2341 atttcccgca tcgtcgatta cctccaccca tgaagatccg gagtaccgga tatgtggctt
     2401 aagccttctg tgccatactt tccgataaga acttggtact tccttaaact attttgtaca
     2461 tcatctctaa taactcatac ccggagaatg aagaatcctc tttccggcac cggaaagagg
     2521 atgaggatag acccaaaaac gaaacttgta aatccttgta aaaccttaga tcagcttctc
     2581 cttggtaaac agaacctcgg cttcgccggc aggtaccggg ggacttatca gatatccctg
     2641 aataatgtcg caattgctct ccgacaggca atccagctgt tcccgggtct cgacaccctc
     2701 ggcgacgacc tttagaccga gttgatgcgc caaggtaatg atggatccga tgaccatcct
     2761 tccattaccg gtttcaatga tgtcatcaac gaaagacttg tcgattttca cgtgatcgat
     2821 gggcaaccgt ttcagatagt tcaatgatga gtatccggtc ccgaaatcat ccaacgagag
     2881 actgacgccg tatttcttta aggcctcaag tttctgagta ttctcctgga acgactccat
     2941 taacagggtt tcggtgatct caagacaaag ggcatccgga ggaagaccgg tcttgtgcaa
     3001 ggcctctttc accttggcca gaaaatca
//
LOCUS       DASVMB010000300         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_37164,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000300 DASVMB010000000
VERSION     DASVMB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37164"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF531_08620"
     CDS             <1..816
                     /locus_tag="VF531_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7714072.1"
                     /translation="VCSEESGNRAVGIDGIMQRGYRIPTCIVMEPSNLEVIPAIQGEF
                     YFRLKVFGKSCHIATRHLAIYPSTVASLPGVNAIEKMTLYMDAFREVEREWGLYQRHP
                     LMEPGTMTLNISEIHGGETFSALAETCEIIGSVLYNPVLSREQAIGEFKAVIDRVGAG
                     DYWLRQHPPELELPYFLPDKIGVNVDPNHDLCKILFDSYKDICGRETRLTGSVSTSDG
                     NYLFEQGIDTVTFGPGRFEMGTHGINEYIPVEDLILCTKVYALTMMGWCGLES"
     gene            840..1589
                     /locus_tag="VF531_08625"
     CDS             840..1589
                     /locus_tag="VF531_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX7714073.1"
                     /translation="MKVLIAGESWQTHQIHMKGFDSFVNSNYETGVQWLKEALESNQI
                     SVDFIPNHEAPLRFPTEIEVLKQYAVVILSDIGSNTLLIHPDTFYRSIRTPNRLSLLK
                     QYVEDGGGLCMIGGYLTFQGIDGRGHWHGTKVEEVLPVTLQATDDRVEVPEGFSPVVT
                     LPQHPVLKGIPQCWPWFLGYNQLTLKPQAELILEYNGDPILAVQQIGSGRTAAFASDC
                     APHWGSVAFINWEYYRQFWAQLITWLAHLSR"
     gene            1794..2555
                     /locus_tag="VF531_08630"
     CDS             1794..2555
                     /locus_tag="VF531_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7714074.1"
                     /translation="MSSITRKNPIPLYLQLEELLRSQIRDHILKPGDPVPTELELQSK
                     YNLSRTTVRQAMANLERDGIINRQQGKGTFISQPRKTQPDLWVLNSFSEEIRRDGGKP
                     GSKVLQLVEIPAPTDIAAFLDVEKDYPLILYRRIRTIDDKPVGIHTVYLNKMIIPGLD
                     PDSLNTDNISLYDILEKDCHLKIGIADEVLESISADASIADTLGIPVGFPVMCIQRTT
                     STIDEIPFEHAKIFVRGDKFKYRARLKRPKGNSRK"
     gene            2676..3110
                     /locus_tag="VF531_08635"
     CDS             2676..3110
                     /locus_tag="VF531_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7714075.1"
                     /translation="MKVKDIMSSQVATVKPDTTVVEAAQLMQKHDVGAVPVCEGPKVV
                     GIVTDRDIVVRNVAHGKDPQTTPVRDVMSSAVNVISPDMSLNQAAELMSDQQIRRLPV
                     VEADHLVGMVSLGDLAAKAKTDVEVAETLGDISHPSEPEKMS"
     gene            3124..3324
                     /locus_tag="VF531_08640"
     CDS             3124..3324
                     /locus_tag="VF531_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714076.1"
                     /translation="MGDIEDRSPVEALQEFVDRKRPALEGLVEDQLGLTTQLDEFLST
                     HLPPESRIAPEEEQDEDNFQPT"
BASE COUNT          871 a          739 c          893 g          863 t
ORIGIN      
        1 gtttgttccg aagagtcagg aaaccgggcg gttggaatcg atgggattat gcagcggggt
       61 tatcgtatac ctacgtgcat tgtgatggaa ccttccaatc tggaggtgat accagccatt
      121 caaggggagt tttacttccg tttaaaggtt ttcggcaaga gttgccatat cgcaacgcgc
      181 catttggcaa tctacccgtc taccgtcgct tcattacccg gagtcaacgc catcgaaaag
      241 atgacgctgt acatggacgc ttttcgcgag gttgaacggg agtggggatt ataccagcgt
      301 catcccttaa tggaaccagg tacgatgact ctaaacatct ccgagattca tggcggtgag
      361 acattttcgg cgttggcgga gacatgtgaa atcatcggca gtgtccttta caatccggta
      421 ttaagccggg aacaagccat cggagagttt aaagcggtga ttgaccgggt tggcgccggc
      481 gattattggc tgcgccaaca tccgcccgaa ctggaactcc cctatttttt accggacaag
      541 ataggggtta acgtggaccc caaccatgat ttatgtaaaa tactatttga ttcatataag
      601 gatatttgtg gtcgcgagac tcggttgacc ggttcagtaa gcacttcaga cgggaactac
      661 ctttttgaac aagggattga taccgttact ttcggccccg gacgttttga aatgggtact
      721 cacggtatca atgaatatat accggtggag gatctgattc tttgcacgaa agtctacgcc
      781 ttgaccatga tgggctggtg cgggttggaa tcctaaaact gatatggagg taattaaaaa
      841 tgaaagtctt gattgccgga gagtcttggc agactcatca gattcatatg aaaggtttcg
      901 acagttttgt gaacagcaat tatgaaaccg gtgttcaatg gctcaaggag gcattggaga
      961 gcaatcagat tagcgtggat ttcattccca accacgaggc gccgttaagg ttcccgacgg
     1021 agattgaggt attaaagcaa tacgcggtag tcattttatc cgatattggc agcaatacac
     1081 tgttaatcca cccggataca ttttatcggt cgatccgcac ccccaatcgg ctaagccttt
     1141 tgaaacagta tgtagaggat ggcggcggtt tatgcatgat tggcggttac ttgacttttc
     1201 agggaattga cggccgcggt cactggcatg ggaccaaggt agaggaggtt cttccggtaa
     1261 cgttgcaggc aaccgatgac cgggtggagg ttcctgaagg attttctcca gtagttaccc
     1321 tgccgcaaca tcctgttttg aaaggtatcc ctcagtgctg gccatggttt ttgggctaca
     1381 accaactgac actcaagccc caggctgaat taatactgga atataacggg gacccaattt
     1441 tggctgtaca gcaaatcggt tcggggcgaa ccgcggcttt tgcctcggac tgcgccccgc
     1501 attggggatc cgtggccttt atcaattggg aatattatcg gcagttctgg gctcaactca
     1561 ttacgtggtt ggcgcattta tcacgttagt cttataagcg aatgagagta gattttgttt
     1621 cccggatgaa acatgatata ataatacgtt gttactatgt ttttgcttcc ggaatattga
     1681 ttcgatgcgt tactccatta tgtggcggag aaaatgatca cgcggcgtaa gcacacatcc
     1741 gggcataaat tttcttcaag cgtccacagc ggttttgtag gaggcaacat aatatgtcaa
     1801 gtataacccg taaaaaccca atacctcttt atttacaact agaagaattg ctgcgatccc
     1861 agatcaggga tcatatattg aaaccgggtg atccggtgcc caccgagtta gagctgcaat
     1921 ccaagtataa tctcagccgc actactgttc gacaggccat ggccaatctg gagcgcgacg
     1981 ggatcatcaa ccggcaacag ggaaaaggta cttttatcag tcagcctcgg aaaactcagc
     2041 cggatctctg ggtcttgaat agtttttcag aggagatccg gagagacgga ggtaaacccg
     2101 gctccaaggt gttgcagctg gttgagattc ctgctccaac cgatattgcc gcttttctgg
     2161 atgtggagaa agattacccg ttaatccttt accggcgaat tcgcaccatc gacgataaac
     2221 cggtggggat tcatacggta tacctaaata aaatgatcat tcccggactc gatccggatt
     2281 ctttgaatac cgacaacatt tccctttatg acattcttga gaaggattgt catttaaaga
     2341 tcggaattgc cgatgaagtt ttggaatcga tctccgctga tgcctcaatc gcggatactc
     2401 taggaattcc ggtgggattc ccggtgatgt gtattcagcg caccacttcc accattgacg
     2461 agatcccgtt cgaacatgcc aagatttttg tacgtggtga taagttcaag tatcgggccc
     2521 ggctgaaacg acccaaggga aacagtcgta aatgaataga gtggtatgat ccggcgggtt
     2581 cgcgggtacc ttattagaaa gctgatttta cggagcattt gatgattcaa caaggccgtt
     2641 tcagctttta aaaaacggga aaggatgaat gatgcttgaa ggtaaaggat atcatgtctt
     2701 cacaggtggc aacggttaaa ccggatacca cggtggtcga ggctgcccaa ttgatgcaga
     2761 aacacgatgt cggagcggtt ccggtctgtg aaggtcccaa agtggtggga atcgtcacgg
     2821 accgggatat cgtggtccgc aacgtggcac acgggaaaga cccccaaacc acaccggtac
     2881 gggatgtgat gagttccgcg gtcaatgtaa tatcgccgga tatgagtcta aatcaagctg
     2941 ctgaactaat gtccgatcaa cagataaggc gtttaccggt tgttgaggcg gatcatttgg
     3001 taggaatggt ttccctgggt gatctggcgg ctaaagccaa gaccgatgtt gaggtggcag
     3061 agaccttagg ggatatctcc catccatcgg aaccggaaaa aatgtcctga ggcggtggta
     3121 aagttggggg atattgagga tagatcgccc gttgaggctc tccaagaatt tgtcgatcgg
     3181 aagcgccccg ccctggaagg attggttgag gatcaactcg gcctcaccac tcaattggat
     3241 gagtttttaa gtacccattt gcccccggaa agccggattg cccccgaaga ggagcaggac
     3301 gaagataatt ttcaaccgac ttaaggaacc aattgaaggg actgactgta tgtcggtccc
     3361 ttgttt
//
LOCUS       DASVMB010000301         7431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_552239,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000301 DASVMB010000000
VERSION     DASVMB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552239"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VF531_08645"
     CDS             complement(<1..607)
                     /locus_tag="VF531_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7714077.1"
                     /translation="MWRAILVDDEEYVRAELAALFPWSRYQFELVGEADNGRAAIDLV
                     NAAKPDLVITDIRMPDMDGLALIARLADVYPQPVVVVVSAYNDFPYVREALRLGAADY
                     LMKAEATLETAAVFLGRLGEILEERYRARNRQAEMTSNIARYHCLAKESFWRDTLTRA
                     SDLTELEARANQLGIVLENMRFGLTFVHIFHAPKTDGNNRTA"
     gene            complement(620..2425)
                     /locus_tag="VF531_08650"
     CDS             complement(620..2425)
                     /locus_tag="VF531_08650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7714078.1"
                     /translation="MKLRRLFTATLRKQLSVSFIFLVISAVSAIGIASITITQQVIRN
                     HTALFGGKMLTQAAYRLGSMIDNAETTVDSLILDRRLAPLLHNLSAANPGVSRAARLA
                     LRDLLLQYRTSLLPGSELILVDSAGNTVTTYTLQTVPRAVIPTSPATKPKVWRLRYLP
                     NYRTGDINVSGRLLELTARIISLPGQSQNGWIILHLDYRMLESIMTNISLQENSLSRF
                     QSDAVVFGPEGQVIFPWIAPSGRILANAYHKLSRQLRNTETIEEKVNGQNYLVIATPV
                     PWTSWEVYISAPTSRLYIGLEQIYRSLLMIGLVCTVIAVFAAAFISFFVTKPVDKLRK
                     AMKRVEDGDFSVTAPESGPMELQTLGRAFNRMLREVDVLTKRLVAEESDRKTAVIKAL
                     QAQIAPHFLFNTLAAMAGMTAKRPPEEVAQALRSLKRLLYLSIGKDGDFVPLADEFEH
                     VRHYLYLMNIRFPGKFLLQMELPEELQRCRTIRLILQPLVENCLQHGFKGAHARQGGV
                     IGVWAVRDGDDVLINVTDNGQGMSPEQMEAVWERDRNRSGIGVRNVDERLKLSFGPDY
                     GLKLTSTHGGGTTVLLRIPFQRSDEPDLLETCNPM"
     gene            complement(2695..2811)
                     /locus_tag="VF531_08655"
     CDS             complement(2695..2811)
                     /locus_tag="VF531_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX7714079.1"
                     /translation="MIAFHNHPSEDPFPSPADFAITRRLQKAGEIKSLKGEV"
     gene            complement(3567..5492)
                     /locus_tag="VF531_08660"
     CDS             complement(3567..5492)
                     /locus_tag="VF531_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013083247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX7714080.1"
                     /translation="MNSDHVGNPENTRDLRKNATLRTALINEANYREINQSQIQNENN
                     LAKAGMLVNADLPVIRSEDSLIPAWDLRGYTFLFNNDIPPTVHPKLWIQGNLNLNVGL
                     FFVTGNIYQVRGFDFANISFIRGNTGWIVIDCLGSKETAEAAIGLVNDYFGEIPVSAV
                     IITHSHEDHYGGIMGVLNFAKADAEIYVPQNFVQSVIDENVYVGIAMSRRGIYMYGVE
                     LPRDAKGQIDNGIGKGPSLGTGTFTQNVREITEQYVKKVIDGVVIEFQLALSTEAPAE
                     MFVYIPGERSLCIAEDANATMHNLLTLRGSKVRDAVAWAGSIQKAIDLWGTTLTSVFG
                     VHNWPRFGNPSCIEYLEKQRDIYQYINDQTLRLINQGYTIEEVGRMVKLPESLADEWY
                     NGEFYGTVIHNVKAVYQRYLGWYNGNPVELNKLPPEESAKKYVEYMGGEAGVLVKARE
                     SFAEGAYQWVAEVTKQVVYANPNNREAKLLCADALEQLGYIAESGPWRNVYLMGAREL
                     RHGILPIKSEFISEDVLNNISLANVLYLLSIRIDGLKAGNLDFQLNFIIPDRGEVAST
                     EVKRGIFRYLSDQLAENAAVTVTMPKQILYELATTNQMPDSSMIIIQGDVNKWHTFLA
                     LRAPINPNFSIMTPVPK"
     gene            5921..6085
                     /locus_tag="VF531_08665"
     CDS             5921..6085
                     /locus_tag="VF531_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714081.1"
                     /translation="MDDWNYLYGAIIEIHISLAMPSVKATVIPVNILKGRAPTARLIR
                     AAFRNGDGNV"
     gene            complement(6999..7295)
                     /locus_tag="VF531_08670"
     CDS             complement(6999..7295)
                     /locus_tag="VF531_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX7714082.1"
                     /translation="MISLNAKRRINGIHIIGVGSLDQVNVEPREVFAAALMNNAAAII
                     AFHNHPSGDPVPSPADFAITRRLQEAGEIMNIRVLDHLVIGEGRFYSMREGGSL"
BASE COUNT         1865 a         1942 c         1739 g         1885 t
ORIGIN      
        1 ctgcagtccg gttattcccg tcggtttttg gcgcatggaa aatgtggacg aaggttaggc
       61 cgaaccgcat attttccaag acgattccca attgattcgc ccgcgcttcc agctccgtta
      121 ggtcggaagc tctggtcagc gtatcgcgcc agaacgactc cttcgccagg caatggtagc
      181 gcgcaatatt gctggtcatt tcggcttgac ggtttcgcgc ccggtatcgt tcttccaaga
      241 tctccccgag ccgtcccaaa aaaaccgcgg cggtttctag cgtagcttcg gctttcatca
      301 aataatcggc cgctcccaaa cggagtgctt cccggacata aggaaaatca ttataggcac
      361 taaccacgac tacaaccggt tgggggtata cgtcagccag ccgggcgatt agagccagtc
      421 catccatatc cggcatccgg atatcggtaa tcaccaaatc cggtttggct gcgttaacca
      481 gatcgattgc agcccggcca ttatccgctt cgccaaccaa ctcgaactga taacggctcc
      541 aggggaagag cgctgccagt tcagcccgga cgtactcttc atcatcaacc agaatcgccc
      601 gccacatgat attgctcctt tacataggat tgcaagtttc aaggagatcc ggttcatcgc
      661 tccgctgaaa cggaatgcgt aataaaacgg tagttcctcc gccatgggta ctggttaatt
      721 tcaacccata atccggtcca aagctaagtt ttaaccgctc gtcaacatta cggacgccaa
      781 tcccggaacg gttacggtct cgctcccata ctgcctccat ttgttcggga gacatcccct
      841 gtccgttgtc agtcacatta atcagcacat catcaccatc acgaacggcc caaaccccga
      901 tgaccccgcc ctgacgcgca tgggcgccct tgaaaccgtg ctgcagacaa ttctccacga
      961 ggggttgtaa tatgagacgg attgtccgac aacgttgcag ctcttcaggc aactccatct
     1021 gcaggagaaa tttgccggga aatctgatgt tcattaaata tagataatgc cggacgtgct
     1081 caaattcatc ggcaagcggg acaaaatcgc catctttacc gatacttaag taaaggagac
     1141 gttttaagga ccgtaaggct tgggccactt cctccggagg acgtttggca gtcatcccgg
     1201 ccatcgccgc cagggtatta aagagaaaat gcggtgcaat ctgtgcctga agcgccttaa
     1261 tgaccgctgt tttccggtcg ctctcttcgg ctaccagacg tttggttaaa acatccacct
     1321 cgcggagcat ccggttaaag gcccgcccca gggtctggag ttccatggga ccgctttcgg
     1381 gcgcggtaac tgaaaaatcc ccatcttcca cccgcttcat cgccttacgg agtttatcga
     1441 cgggtttggt tacaaaaaac gagatgaaag cagcggcgaa aacggcaatg accgtacaaa
     1501 ccaaaccaat catcaacagg ctccggtaaa tctgctctaa gccgatatag agacggctgg
     1561 tcggcgcgga gatatatacc tcccaggaag tccagggaac cggagtggca atgaccagat
     1621 agttttgacc gttgactttt tcctcgatgg tttccgtatt ccgaagctgc cgcgacagtt
     1681 tatgataagc attggccaga atccggccgg atggggcaat ccagggaaag attacctgtc
     1741 cttcgggtcc aaaaactacc gcgtcgcttt gaaaacggct caaagaattt tcctgcaaag
     1801 agatgttggt cataatcgac tccaacattc tgtagtccaa atgcaagatg atccagccgt
     1861 tttgagactg acccggcagg ctgataatcc gggcggttaa ctccaacaag cggccggaaa
     1921 cgtttatatc gccggttcta taattgggga gataccgcaa gcgccagact ttgggtttgg
     1981 tagcgggaga ggtcgggatg acggcccggg gaactgtctg tagcgtataa gtggtcacgg
     2041 tattcccggc cgaatcaacc aggatcaatt ctgagcccgg cagtaaggac gtccggtact
     2101 gaaggagtaa atcgcgcagt gccaaccggg ccgcccggct caccccggga ttcgccgcgg
     2161 ataaattatg taacagcggg gccaaccgcc ggtctaaaat cagggaatcc acagttgttt
     2221 cggcattatc aatcatacta cccaaccggt aagcggcttg agtcagcatt ttaccgccaa
     2281 acaacgccgt atgattgcgg atgacttgtt gcgttatggt aatggaagca attccaatgg
     2341 cggaaactgc actgataacc agaaatatga aagaaaccga caactgcttg cgcagggtgg
     2401 cggtgaataa ccggcgaagt ttcatgaacg gctcccatcg cttcaatcgt cactgattcc
     2461 aatgtgtcta atttctgtta atgtcgcaat aaatagtttt caatgaataa aaatgattcc
     2521 atatcttttt aattcgattc tattccggct acatccttct gacccaatta tgtaaattaa
     2581 tttatctgtt gaaaagggat ttgaacttgc cggttccgtc aggtaaagtt ggccatcctt
     2641 cgatgagtat atacgtccaa cccctcgtac tttttcccta tatattttca aacttcacac
     2701 ttcacccttc aaacttttga tctccccggc cttttgcaac ctcctggtaa ttgcgaaatc
     2761 cgccgggctg ggaaacggat cttcactggg gtgattgtgg aaggcgatga tcgaggaaag
     2821 tttggatgct gatgatggct atgggagtca gttctgtcga cgacggggga cgtcacccac
     2881 tcccccgcac cctctagggc caaagggttc gcgttctgtg gaacgcgcag tcggccaccc
     2941 tttggaatcc gccgacctgg aaccgaggtt ccaggacctc cgcgcgcgtt tcatgcgcga
     3001 ttcatcccgg tttttagaac gtcgcgcgca ctttgtgcgc gacgccatcc ttggctgcct
     3061 ctccaaagga gaggattcga ctagcaacta agtcaacgct tccgaccgcg accgctattt
     3121 ttcatcctcg aaaagtcgaa ctccaaagag ttcgcagcgg cgcccggact ccttccatgg
     3181 agtccggaga cgaggactag ctctagatgc cttaaggcaa tttgatgtgt aaaattagct
     3241 ctaaaaaact tctttgaaag cctaacacaa ccaacaagga tgttggtggc gcggcttcga
     3301 ttcttggatg aatcatcggc ttgtttacaa gccgcgccgt cgagaagaaa ggaccactct
     3361 agaggccgga gacacgagat cgcaggccaa taggccctat tacatagcat tctagggttc
     3421 acctccccgg cccaaggatg ggcggggata aggggggtac cgagggcctc acccaaaata
     3481 ggcgtcgtga catagcaatt ttcccctctc ccagtgtttt cgtgggagag gggaataaag
     3541 gggtgaggtg tccggccacc cgttaactat tttgggaccg gtgtcataat actgaaattt
     3601 ggattgatcg gagcccgtaa cgctaaaaat gtatgccact tatttacatc tccctggatg
     3661 attatcattg aactatccgg catttgattc gttgttgcca gctcatacag tatctgtttt
     3721 ggcatcgtaa cagtaacagc cgcattttcc gccaattggt cagataaata tcggaaaatc
     3781 ccccgtttta cttctgtgga agctacctct ccccgatccg gaatgataaa attaagctga
     3841 aagtccaaat tcccagcctt taatccatcg attcggatac tcaataaata gagcacattg
     3901 gccagtgata tgttatttag gacatcttcc gaaataaact cacttttgat gggaagaatg
     3961 ccgtggcgaa gttccctggc tcccattaaa tatacatttc gccaaggacc cgattccgca
     4021 atatagccca attgctctaa ggcatcggca caaagcagct ttgcctcccg attgtttggg
     4081 ttggcataaa caacttgttt ggttacctcg gcaacccatt gatatgctcc ctccgcgaag
     4141 gactccctgg ctttaactaa aacccccgct tcgccgccca tatattccac atatttcttg
     4201 gctgattctt ccggaggtaa tttatttagc tccaccgggt tgccgttata ccagccgaga
     4261 tacctctggt aaaccgcttt gacattatga atgaccgttc cgtaaaattc gccattatac
     4321 cattcatccg ccagactctc cggaagttta accatccggc cgacctcttc aatggtatat
     4381 ccttgattta taagtcgcaa agtttgatca ttgatatatt gatagatatc cctttgtttc
     4441 tccaaatact caatacaaga tggattcccg aaacggggcc agttgtgaac cccaaatacc
     4501 gaagtcagag tagtacccca taaatcaatt gccttttgaa tactcccggc ccaagcaacc
     4561 gcatctctga ctttggaccc ccgtaaagtt aacaagttat gcatggtggc attggcatct
     4621 tcggcaatgc ataaagagcg ttctcccggt atatatacaa acatttccgc gggtgcttcg
     4681 gtgcttaggg ccaattggaa ctcgataacg accccgtcaa ttactttttt tacgtattgt
     4741 tccgtaattt cccgaacgtt ttgagtgaat gttcctgtgc cgagcgatgg accctttcca
     4801 atgccgttat cgatctgtcc tttcgcatcc cgaggcagct ctacaccgta catatatata
     4861 ccacgccggg acatggcgat acctacatat acattctcat caatcacgga ttgaacaaaa
     4921 ttttgaggga cgtaaatctc agcatctgct ttggcaaaat tcagaacacc cataattcca
     4981 ccgtaatggt cttcgtgaga gtgagtaatg atgactgcgc tgacaggaat ttcgccaaaa
     5041 taatcattaa ctaaaccgat agctgcctcc gcggtttctt tggaacctaa acaatcaatg
     5101 acaatccagc ccgtatttcc ccggataaag gagatattgg caaaatcaaa acccctaacc
     5161 tgatagatat tgccggtaac aaaaaataag ccgacgttta aatttaagtt tccttgaatc
     5221 caaagcttgg gatggaccgt gggaggtata tcgttattga ataagaaagt atagcctctt
     5281 aaatcccatg ccggaatcaa agaatcctcg cttcgaatta ccgggaggtc ggcattcacc
     5341 agcattcccg ctttggccag gttattttcg ttttggattt gactttgatt gatttcccga
     5401 tagtttgctt cattaattaa cgctgttcta agtgtcgcat tttttctcag atctctggta
     5461 ttttccggat ttccgacatg atcagaattc accgccaaac cctcccaacc acttccggtt
     5521 attttgcgtt cgcgtaatac cttatgcatt agcccaaatg gaagccattg cttaaataga
     5581 aaaataaaaa agccggtccg gatgctggct gcatcctaac cggcgggttg gactatggat
     5641 tggaacttgg gtgatataaa ttattacatt tctgaaaatt aaagtcaatc atctccagct
     5701 gcgattcgag tattcccagc tagattgatt gatcgacgtt gcggcttgaa attgggtgtt
     5761 tacctaggag ccgaaattat ttaccccaag cattttttac agttcactct tcaaccttcg
     5821 cactttactg ctctaccaat cccatgacaa tattacgaac gctttcttta tacttatata
     5881 cctttggttc aaccgggtaa tatttattgg cccaggaggg atggatgatt ggaattacct
     5941 ctatggtgcg attatagaaa tacatatttc tttggccatg ccgtccgtta aagccacggt
     6001 tatcccggtc aacattctga aaggaagagc gcccaccgcc aggctaatcc gagcggcttt
     6061 ccgcaatggt gatggcaatg tgtgagtcag ctctgatctc gacgggggac gtcacccact
     6121 cccccgcccc cctagggcca agggattgga ccggcaatcc cttggaaccc accggcccgg
     6181 gggcttggcc cccgggtacc ccctgatatc ggcgctcatc cttgaccgcc gtcggagtgc
     6241 ttcgcgatta tacagacgcc tacctccatg tagccgtctt cttgggtgat tctttgtgcc
     6301 gacggccgtg attcgtccag aatcgaggcc gcgtggtcaa cctccctgtt gaccatttcg
     6361 gggtaaaaaa gaatcaaatt tgactggcaa ctaagtcaag gcttccgaac gcgaccgcta
     6421 tttttcatcc tcgaaaagtc gaactccaaa gagttcgcag cgtcgcctgg actccttcca
     6481 tggagtccgg aaatgttgga ctagctggat gccttaaagc agataaattt ataaaattag
     6541 tccaaaaaac ctcttcgaaa gacaaaaacc accaacaggg atgttggtgg cgcggcttcg
     6601 attccggatg aatcatcggc ttgtttacaa gccgcgccgt cgggggaaaa ggactaccat
     6661 agaggccgga gacacgaggt cgcaggccag ataggcccaa ctatttaaca ttctaaggtt
     6721 cacctccccg gcccagggat ggccggggat aaggggagta ccggggggct cagcggcata
     6781 ggcgactttg acagagcacg tttcccctct cctacttgct tttagaagga gaggggaaaa
     6841 aaggggtgag gtggccgctg ggttccaagg gattggcggc tcaatccctt ggccctaagg
     6901 ggtgcgggga agtgggagac gttccccgtc ggcaactatg attccttagt ttctccaacc
     6961 ctcgcacttt ttcccttttc atttttccct tttcaatttt ataaacttcc gccctccctc
     7021 atactataaa acctcccctc cccgatcacc aaatgatcca gcaccctgat attcataatc
     7081 tctccggcct cttgcaacct tctggtaatc gcaaagtccg ccgggctcgg caccgggtcg
     7141 ccgctggggt ggttgtggaa ggcgatgatc gccgctgcgt tattcatcag cgcggcggca
     7201 aaaacctccc gcggctcgac attaacctga tcgagactgc cgacgcctat tatatgaatg
     7261 ccattaatcc tccgtttggc attgagggag atgataccga attttaaaag agcatatgct
     7321 cttttttaag agttgcaatg caactctact ctaccggttc ggaggagaga tcgaggaaag
     7381 tttggagcgc tttgtaacag gccagaggac aggtgattga acaggagccc a
//
LOCUS       DASVMB010000302         8680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_112472,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000302 DASVMB010000000
VERSION     DASVMB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112472"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VF531_08675"
     CDS             complement(<1..611)
                     /locus_tag="VF531_08675"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7714083.1"
                     /translation="MNRKLAVLGLGIENLALVKYLAGKGDELTVCDSRDRIALGERYE
                     LLTQLGVTFRLGPDYLERLEDFDTVFRSPGLPLFQPELRQAEAKGVEVTSAMRLFLDL
                     CPCPTIGVTGSKGKGTTSSLIHRILTLSQAKKGSQAFLGGNIGIAPFEFLPRLRPEDR
                     VVLELSSFQLEDCHRSVDIAAITNITEDHLAPADPLNPNYHKSR"
     gene            1156..2823
                     /locus_tag="VF531_08680"
     CDS             1156..2823
                     /locus_tag="VF531_08680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7714084.1"
                     /translation="MGLTNKQDRVLLIPLGGLGEIGKNMMAIEYNKEIIVIDAGLMFP
                     DEDMLGIDLVIPDITYLVENRERVRGIFLTHGHEDHIGALPYILKQLNVPIYGTKLTL
                     GLARGKFEEHGMVRDVTMHCIKAGERVDIGRFTVEFIHVNHSIADVVALAVHTPVGII
                     LHTADFKFDHTPVDGKVTDFRKLAELGDKGVLALLSDSTNVERIGYTPSERELAQTFD
                     ELFLQAEGRILVATFASNIHRIQQIVDTAFHFNRKVALAGRSMVNVATIAMNLGYLQI
                     PEGVLIELDDVDRYAPEQLTVITTGSQGEPMSALSRMATAEHKKVTIVPGDTVIISAS
                     AIPGNEQLVARTINHLFKLGAQVIYESAQGIHVSGHASQEELKLMLNLTRPKFFMPVH
                     GEYRHLVQHARLAVDVGINSENVIIAENGDVVEVNKDRIRISSKVPSGKIFVDGLGVG
                     DVGNIVLRDRKQLSQDGILIVVVTIDKQSGKIVAGPDIVSRGFVYVRESEELIDEAKE
                     KVKQALERCSEYSDWATMKSQIREILSKHLYGKMKRRPMILPIIMEV"
     gene            2951..4072
                     /locus_tag="VF531_08685"
     CDS             2951..4072
                     /locus_tag="VF531_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714085.1"
                     /translation="MFLTGKGDHAILKPDNLIDSVNLKDGINFRIGVIPTTTIKDVAK
                     YAGVSQATVSYVMNNNTDEVGYSTQQKVLEAVKILNYHPNANARSLKSKNTRNIGLVM
                     LHTETKQLLEDPWIIGLLAGIADISREAGYSIVIDLIMKWNEETYSRIFRGQKTNGTI
                     ILGAAISDKIGDKLTTDKFPHIFIDRYTEDTKTNCICIDNEGGAKKAVHYLASLGHQR
                     IAYIGGIGDFASGLYRKKGFTEAMKELALPINPAYILEGKWSEESGYNALFQLFDLEI
                     PPTAVFCANDRMAIGALQAAYDRKIKVPDQISVMGFDNVQFSAYTTPPLTTLTVPIYE
                     IGKFAANQIIAIDNGKKFFRKEVFDVNIVIRDSTAPPAL"
     gene            4242..5564
                     /locus_tag="VF531_08690"
     CDS             4242..5564
                     /locus_tag="VF531_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714086.1"
                     /translation="MKRFLLIFLVLVMVFSCFTVLAAPKVTLRYALWDGNQKPAMEAA
                     IAAFMKANPGIEVKIELTEWNDYWTKIQTGVAAGTMPDVFWGHIAYFSSLASKGALMD
                     LNPLIKKDKIDLSAYYPGLTANWSYKGKQFGLAKDWDTICYMYNKDMFAQAGITAPEK
                     WTWNGKDGGSFLQDLKKLTIDANGKNATEDGFDKTKIVRYGIAGNNAGEIQTGWINFV
                     WMNGTPGAGVLDKPFGTKFTLDDPKAVEALQFYFDLTNKYSVAPQTDNSSFAGGAWDL
                     FNAKKIAIYPCGSWMLGAARSSVKFGWDVAPLPAGPAGRISAFNGLAHLIASKTKYKD
                     AAWKLVKWMDGPQGQKIVAGYGCCFPSIKSIVPVYMAATKEKGPAHLQYYFDEAAGKT
                     GEYPMHVNWAQMWDVIQRELNAAASGSETVKEATANIKAEVTPLLIVK"
     gene            5649..6593
                     /locus_tag="VF531_08695"
     CDS             5649..6593
                     /locus_tag="VF531_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714087.1"
                     /translation="MDNQELELGSYQLLHPEKKAKNKQRFRQALAGYLFIAPNFLGFF
                     VFALAPIVATIVLSFSDWDLVNPLKWVGWINYKNIFASPASWASLKQTILFTVLNVPI
                     QCFIALGIALILNQKLKGLNLFRTLFLLPWVCMPIAHALVWQWMYNYSFGILNHLITS
                     FGFQGVRWLNNPAVAFFAVMFVNIWSYLGMHIVLLLAALQNVPGELYEAALVDGANAW
                     VRFWKITFPMISPVFFYDIVVNMIGTLQIFDLPFALTNGGPGNVNLYFNLLLYRKAFS
                     YFQMGEACAMGVILFALIAFLTFITFKYIGSRVNYDQV"
     gene            6616..7449
                     /locus_tag="VF531_08700"
     CDS             6616..7449
                     /locus_tag="VF531_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714088.1"
                     /translation="MKTAGKKKFTKLLTYIGIITFLFFFTFPIIWELSTSFKTNDESF
                     AGYNLIPHHFTIEGYLKALTESSMGTYFSNTVFVVFCIALGSAIACSMAGFAFGQLKF
                     PGKDFLFFLYITTLMIPVSVTIIPSYIIMIKLGWANSYYALIVPFLFGNAFGTFLLRQ
                     FYQTLPRELFESATIDGAGYFQTWLHIMAPLTAPAIATFALMTIQSQWNSLLWPLVVT
                     QSPQFKTLALGLSDFRLMRVVNWNAMMAAVMIATAPMIILLFVANKFFLRSIQLSGIN
                     R"
     gene            7481..>8680
                     /locus_tag="VF531_08705"
     CDS             7481..>8680
                     /locus_tag="VF531_08705"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714089.1"
                     /translation="MNRLSRLLLFFIIFNTAFMTYAATLPEKHTEDFDQSELSKEWHW
                     IREDKDSWSLTKKPGYMTITTRVGDIWGGINDASNVLLRSVPCGNADFEFTAKIEFTP
                     TANWHQLGMLVAQDLDNYLVMKALYSDGPKLQWGKEVKAYFTSTSTLAEFPQIQYLRI
                     TRKNGVYTGYFSEDNQKWTEVGSYSDVILNNISVGFLVCNGGSSQISSTDAKIDWLNI
                     AYDEKAAILPKAPDTRVLTYTNAPVENPLKGFMPYLDARNVSKPGHDNYDRFPYSMEY
                     FYIPLRQLMVDDNKFDWSDLEMNLNIIASRGNQAVFRVYLDYPQKTTGIPDFLLKKGL
                     KTHQYSQFGAGTCPDYNDSKLLQALDNFIAALGAKYDGDPRIGFIEVGLVGFWGEWHT
                     TGSDWMPT"
BASE COUNT         2308 a         1832 c         2080 g         2460 t
ORIGIN      
        1 cgcgacttat ggtaattagg gtttagagga tcggcaggtg ccagatgatc ctcggtgata
       61 ttcgtaatcg cggcaatgtc gacacttcga tgacaatctt ccagttgaaa actggatagt
      121 tccaacacca cccgatcttc cggtctgaga cgggggagaa actcaaacgg ggcgatcccg
      181 atattaccac ccagaaaagc ttgggaaccc tttttagcct ggcttaacgt caagatgcgg
      241 tggatgagtg aagaggtagt tccttttccc ttggatccgg tcactccgat ggtagggcag
      301 gggcataaat ccaagaataa ccgcattgca ctggtaacct ctactccttt ggcctcggct
      361 tgtcttaact ccggttgaaa aagcggcagt ccgggagatc ggaaaaccgt atcaaaatcc
      421 tcaagtcttt ccagataatc cggtcccaat ctaaaagtta cacctaattg ggttaagagt
      481 tcgtaacgtt cgccaagagc gatccgatca cggctatcgc agacggtgag ctcgtcaccc
      541 ttaccggcca gatatttaac gagggctaaa ttctcgattc ccaaaccgag tactgcaagc
      601 ttacgattca agatgccgct ccttccttga taaaacccga ttcgatcgcg tcgtacactg
      661 gaggccatct gagtgaacag atctttccgt gcttttttaa gaaaatcgta atgcatgtac
      721 ttgggcacga cttgccgaat cgaggtttac gaaagccaga tggattttta gtttgaaaaa
      781 aaaggcttaa ggtaattgta gccagattgt gccataaaat caaggctttt caatttattt
      841 tacagttgac ttgtgatcat aagggaaatc cgctataata aaggttataa tttattgtgc
      901 ggaaaaggcg catcataatg cggggttcat ttacaactct catttgagcg ccggtcgtgt
      961 cctcggattg caaatacgta accgggagcg gccggatttg gtttcatcga ttttacgcca
     1021 taagcggcgc gcgggatgtg gatttgatag agacaacccg accgtggaat tgcggggtag
     1081 cgccctgttc ggagagtaat tccacgggaa ttggaaaacc acccgtttac agcacaacag
     1141 gaggtgtttt tttctttggg tcttaccaat aaacaagacc gggttttact gattcccctg
     1201 ggaggactgg gcgagatcgg taaaaatatg atggccatcg agtataacaa ggagattatc
     1261 gtcatcgacg ccggattgat gtttccggat gaagatatgc tgggaatcga cttggttatt
     1321 ccggacatca cctatctggt agaaaaccgt gaacgagtcc ggggcatctt tttaacccac
     1381 ggccatgagg accatatcgg tgctctgccc tatattttaa agcagttgaa tgtccccata
     1441 tacgggacta aactcactct gggactggcc cggggtaagt tcgaagagca tggcatggtt
     1501 cgggatgtca ccatgcattg tattaaagcg ggagaacgcg tggatatcgg ccgctttacg
     1561 gtggaattta tccatgtcaa ccatagtatc gctgatgttg tggccttagc ggttcatact
     1621 cctgtcggca tcattttgca tacggcagac tttaaatttg accatacccc ggttgatggg
     1681 aaggtcaccg acttccggaa actggcggaa ttaggcgata aaggagtgct ggcgttactc
     1741 tcagacagca ccaacgtgga gcgcattggt tatactccgt cagaacggga attggcccag
     1801 accttcgatg aactctttct tcaggccgaa ggcagaattt tagtagccac ttttgcctcc
     1861 aatatccacc ggattcaaca aatcgtcgat accgcttttc actttaaccg taaggtggct
     1921 ttagcgggcc gaagcatggt taatgtagcc accatcgcta tgaacctggg atatttgcag
     1981 atcccggaag gggttctcat tgaactggat gatgtggatc gttacgcccc ggaacaattg
     2041 acggtgatca ccaccggcag tcagggtgag ccgatgtcgg cgctctcgcg gatggctacc
     2101 gccgaacata agaaggtcac gattgttccc ggagataccg taatcatctc agcctcggcg
     2161 attcccggca atgaacaatt ggtggcccgt accatcaatc atcttttcaa actcggcgct
     2221 caagttattt atgaatcggc ccaagggatc catgtttcgg gacatgccag ccaggaagag
     2281 ttaaaactga tgcttaattt gacccggccc aagtttttca tgccggttca cggcgaatac
     2341 cgccatctgg tgcagcacgc ccgtttggcc gtagatgtcg gaatcaacag cgagaatgta
     2401 atcattgccg aaaatggcga tgtggttgaa gtcaacaagg accggatccg gatctcgagt
     2461 aaagtgcctt cgggcaagat tttcgtcgac ggccttggag tcggggatgt cggcaatatc
     2521 gtgctccggg ataggaagca actttcccag gatggtatct tgattgttgt agtcactatc
     2581 gataagcaga gcggcaagat cgtggccgga ccggatatcg tctcccgggg attcgtctat
     2641 gtccgggaat cggaggagtt gatcgacgaa gccaaggaaa aggtgaagca ggctttagaa
     2701 cggtgcagcg agtattcgga ttgggcgacg atgaagagcc agatccgtga aatcctgagc
     2761 aagcacctgt acggcaagat gaaacggaga ccgatgattc ttcccatcat catggaagtc
     2821 taagatcgaa aaaagaccga ggaagcgtag gctcctcggt cttttttttc atcgtgtttg
     2881 gctatctaat gtcgtgccct tcatggcgta aaaaatgtgt gtttaaaatt ttcgtaaaag
     2941 attgatcggg atgttcttga caggaaaagg agatcatgct attcttaaac cagataactt
     3001 aatcgattca gttaatctaa aagacggaat aaattttcgt ataggtgtga ttcccacgac
     3061 taccattaaa gatgtagcaa agtatgcagg cgtttcccag gcaaccgtgt cttacgtcat
     3121 gaataataat acggatgaag ttggctattc aactcagcaa aaagtgttgg aagccgtgaa
     3181 aatccttaac tatcatccca atgccaatgc ccggagttta aaatccaaaa acacccgaaa
     3241 tatagggctc gtaatgcttc atactgaaac gaaacaattg ctggaagacc cctggattat
     3301 tggattactg gccggtattg cggatatttc aagagaagca ggatatagta ttgtcattga
     3361 tctaatcatg aaatggaatg aagagacata ctcccggata tttagggggc agaaaaccaa
     3421 tggtacaatc attttaggag cggcgattag tgataaaatc ggtgataagt taacgactga
     3481 taaattccct catatcttta tcgatcgtta tacggaagat accaagacca attgcatttg
     3541 tattgacaat gaaggtggag ccaagaaagc ggttcattat ttagcatcgc tcggacatca
     3601 aagaattgcc tatatcggag gcattggaga ctttgcttcg ggtttatacc ggaagaaagg
     3661 ttttaccgag gcgatgaagg aacttgcttt gccgattaac cccgcttata tcctggaagg
     3721 taagtggtcg gaagaatccg gctacaacgc cttgttccaa ttgtttgacc tggaaatacc
     3781 gccaaccgcg gttttttgtg cgaatgatcg gatggctatc ggggctcttc aagcagccta
     3841 tgacagaaag atcaaagtac cggatcaaat atcggttatg ggtttcgata atgtgcagtt
     3901 ctcagcatat acaactccac ctttaactac tttaacagtc ccaatttatg aaatcgggaa
     3961 atttgctgcc aatcaaatca tcgctatcga taacggaaaa aaatttttca ggaaagaagt
     4021 ttttgatgtg aatattgtca ttcgcgattc gacagctccg cccgctttat aatcgatttt
     4081 atgaccgtga ccggtgatta cttaatagga aaaggggtaa gtttgcgaac ttaaacgttt
     4141 cggttgttat attcggtttc tgccgggttg aaggaaagcg aggggttccg caacagtcgg
     4201 gtgggaacca atataaaaat aaaaaaataa ggagggttat catgaaaagg tttttactta
     4261 tttttttggt tttggtcatg gttttttcct gttttaccgt attggccgcc ccgaaggtta
     4321 cactccgtta cgcactgtgg gatggcaacc agaagccggc gatggaggcc gctattgcag
     4381 cttttatgaa agcgaatccg ggtatcgaag ttaaaattga attaaccgag tggaacgatt
     4441 attggaccaa gattcaaacc ggtgttgccg caggaacgat gcccgatgta ttttggggcc
     4501 acatcgctta cttttcaagt ttagccagca aaggcgcgct catggatctc aaccccctaa
     4561 tcaaaaaaga caagatcgat ttaagcgcat attatcctgg tctgaccgct aactggagtt
     4621 acaagggtaa acaattcggt ttggccaaag attgggatac tatctgttat atgtacaata
     4681 aagatatgtt tgctcaagcc ggtatcaccg ctcctgaaaa atggacttgg aacggtaaag
     4741 acggcgggag ctttttacag gacctgaaga aattaaccat tgatgccaat ggcaaaaatg
     4801 ctactgaaga cggcttcgac aaaaccaaga tcgtccgtta cggtatcgcc ggtaacaacg
     4861 ccggagaaat tcaaaccggt tggatcaatt ttgtttggat gaatggcacc cctggagccg
     4921 gtgttttgga taaaccattt ggcactaagt ttaccttgga tgatcccaag gcagtggaag
     4981 cgctccaatt ctattttgat ttgaccaata aatatagtgt tgcaccgcag accgacaatt
     5041 caagtttcgc cggtggagct tgggatcttt tcaatgcgaa aaagatagcg atttatccgt
     5101 gcggttcctg gatgctaggc gcggcgcgtt ccagcgtcaa atttggatgg gatgtcgctc
     5161 cgcttccggc ggggcctgcc ggccggatca gcgcttttaa cggtttggcc catttgatcg
     5221 cttccaagac aaaatataag gatgctgctt ggaaattagt taaatggatg gatggccccc
     5281 agggtcaaaa aattgtcgcc ggatacggct gctgcttccc ttccattaag tcgattgttc
     5341 cggtctatat ggctgctacg aaagaaaaag gtccggccca tctccaatat tattttgatg
     5401 aagccgcggg taagaccggt gaatatccga tgcatgtcaa ttgggctcaa atgtgggatg
     5461 ttatccagcg tgagttgaat gcggctgcct cgggatccga aactgttaaa gaagcaacgg
     5521 cgaatatcaa agccgaagtt actccattac ttattgtaaa ataaataata cctatattag
     5581 cgggagaacc ccggggaacc ggcgggtttt cccgctttct tgttaaagtt gtcagaatgg
     5641 aggtcgccat ggataatcaa gaactggaat taggcagtta ccaattgctt caccctgaaa
     5701 aaaaggctaa aaataagcaa cgctttcgtc aggcgttagc gggttatttg tttatagccc
     5761 ctaatttttt gggttttttt gtctttgcgt tagcgccgat cgttgccact atcgttcttt
     5821 cttttagcga ttgggacttg gtaaacccat taaaatgggt tggttggatt aattataaaa
     5881 atatctttgc ttcccccgca tcctgggctt ctttaaaaca gacaatcctg tttacggtgt
     5941 tgaatgttcc gattcagtgc tttattgcac tgggaatagc gttgatatta aatcagaaat
     6001 taaaaggttt aaatcttttc cggacccttt ttcttttacc ttgggtttgt atgccgattg
     6061 cccatgcctt ggtctggcaa tggatgtata attattcgtt tggaattttg aatcatctca
     6121 tcaccagttt tggttttcaa ggggttagat ggttgaataa tccggcggtt gccttttttg
     6181 cggttatgtt cgtgaatatc tggagttatt tggggatgca tattgtactg ctcttggccg
     6241 ccctccaaaa cgtaccgggg gaattgtatg aagctgcgtt agtggatggc gccaatgcct
     6301 gggttcgatt ttggaaaatt acttttccaa tgatcagtcc ggttttcttc tatgatatcg
     6361 tggtcaatat gatcgggaca ttacaaattt ttgatcttcc ttttgcattg acaaatggcg
     6421 gtcctggtaa tgtcaattta tattttaatc ttttacttta ccgcaaagca ttttcctact
     6481 tccagatggg tgaagcttgc gcaatgggcg taattttatt tgccttgatt gcttttttaa
     6541 cctttatcac cttcaaatat ataggctcac gggtaaatta cgaccaagtt taattttcca
     6601 aaaggggggg ccttgatgaa aacggccggc aaaaaaaaat ttacgaaact tcttacatat
     6661 atcggcatta ttaccttctt gttttttttc acatttccaa tcatatggga actgagtacg
     6721 tcatttaaga caaatgacga aagtttcgcc ggatataacc ttattccaca tcactttacc
     6781 atcgaaggct atttgaaagc tttaactgag tcgagtatgg ggacttactt ttcgaatacc
     6841 gtttttgttg ttttctgtat tgccctgggc tcggcaatcg cttgttcgat ggctggtttt
     6901 gcattcggtc aattaaaatt tccgggtaaa gattttttat tcttccttta tattaccaca
     6961 ctaatgattc ctgtttcagt aacgattatc ccttcttata ttattatgat caaacttggc
     7021 tgggctaaca gctactacgc ccttatcgta ccgttccttt ttgggaatgc tttcggcacc
     7081 tttttattgc gccaatttta tcagactttg ccacgggaac tatttgaatc ggcaaccatt
     7141 gatggggccg gttatttcca aacctggctt catataatgg cacctttaac cgctccggcg
     7201 atagcgactt ttgccctgat gactatccaa agccaatgga attcattact ttggccattg
     7261 gtggttaccc aaagccccca atttaaaaca ctggcgctcg gattatccga ttttcgctta
     7321 atgcgggttg tgaattggaa tgcaatgatg gctgcagtga tgattgctac ggcgccaatg
     7381 atcattttgt tatttgtcgc caataaattt ttcttacgaa gcattcaact atccggaatc
     7441 aatcgctaga attgtgaaat ggaataaagg gggattatta atgaacagat tatcccggct
     7501 gcttttattc tttatcatat tcaataccgc ctttatgacc tatgcagcca cccttcccga
     7561 gaagcatacc gaagattttg atcaatccga actgtccaaa gagtggcatt ggattcggga
     7621 agacaaagat tcatggagtc tgaccaaaaa gcccggatat atgacgatta ccaccagggt
     7681 cggagatatt tggggcggaa ttaacgatgc ttccaatgtt ttattgcggt cggttccgtg
     7741 tggtaatgcc gatttcgaat ttaccgccaa aatcgagttt actccgaccg ccaactggca
     7801 tcaactggga atgttggttg ctcaggattt ggataattac cttgtaatga aggcgcttta
     7861 ttcggatggc ccaaagctgc aatggggtaa ggaagtcaag gcttatttca cttcaacgtc
     7921 gacccttgct gaattcccgc agatccagta tttgcggatt acccgcaaaa acggcgtcta
     7981 taccggatat ttcagtgagg ataatcaaaa gtggactgaa gtcggatcat acagcgatgt
     8041 aattctgaat aatatttcag tcggattttt ggtttgcaac ggcggcagtt ctcaaatttc
     8101 cagtactgac gccaagattg actggttgaa tattgcctat gatgaaaaag cggctatatt
     8161 gcccaaggcc cccgatacca gggtgttgac ttataccaat gctccggttg aaaatccact
     8221 gaagggtttc atgccatatc tggatgcccg gaatgtttca aaaccgggcc atgataatta
     8281 tgatcgcttc ccctacagta tggaatattt ttatattccc ctccggcaat taatggttga
     8341 tgacaataag ttcgactggt ccgacctgga aatgaattta aatatcattg cctccagggg
     8401 gaatcaggca gtgttccgcg tttatcttga ttatccccag aagactaccg ggattccgga
     8461 ttttttgctg aaaaaagggc tcaaaactca tcaatattct caatttggtg ccggtacctg
     8521 tccggattat aatgattcga agttacttca ggcgctggat aattttattg ccgccttggg
     8581 ggcgaagtat gacggagatc cgcgtatcgg cttcatcgaa gttggcttag tcggcttctg
     8641 gggcgaatgg cacactaccg gaagcgattg gatgccgacc
//
LOCUS       DASVMB010000303         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_259132,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000303 DASVMB010000000
VERSION     DASVMB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259132"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..979
                     /locus_tag="VF531_08710"
     CDS             104..979
                     /locus_tag="VF531_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX7714090.1"
                     /translation="MIQQLSVKELNSDKLKDFLLINLGLALVAAGIHFFKIPNHFATG
                     GVSGLAIVIRHFFPRIAVGPLMLIINILLLIAGYALIGRIFGSKTVYSSFALSGMVWL
                     LGKIYPVNTPFTNDTMLELILSILLPAVGSAITFNLNASTGGTDIVAKVLNKLTDIDI
                     GKALLLADLAITLSAGFVYGIRIGMYSFLGLVLKGFVIDSVIEGLNLRKQLVIISEKP
                     ELIQEYIINNLHRGATIHIAYGAFTDDEKHVITTVVTRGQAIALRKYIREIDSKSFIT
                     ITNTSEIIGKGFRSI"
     gene            complement(1069..1869)
                     /locus_tag="VF531_08715"
     CDS             complement(1069..1869)
                     /locus_tag="VF531_08715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7714091.1"
                     /translation="MKIYFIRWALLRRFCVGIVVAGLLAGTYRYLSIPVYDSSLTWKM
                     GVVREAKVNEKLIALTFDDGPSPTFTNKILDLLKQYHAKGTFFIVGAQAEKYPEIIER
                     ELREGHEIANHMYQHREVYGMPIDELKEDLEHSHQAIYQITGQVMRLYRPTSGYCNER
                     IYELACSLNYTVIIWTWGLDSRDWTGMNGRSIAGKIIKVVKPGGIVLFHDLGGNRNNT
                     LQALEILVPELISEGYQLVTVSKLLWQAKMDAQGLQRNRITVSGFRKN"
     gene            2084..3019
                     /locus_tag="VF531_08720"
     CDS             2084..3019
                     /locus_tag="VF531_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7714092.1"
                     /translation="MKKGYWYIFFSTILFSTMEIALKLVTNDFHPIQLTLLRFLIGGL
                     ILFPLAFQNLKTRGVSLNAADFRYFAITGLICVVISMTFYQLAVLYSKASTVAILFSC
                     NPAFVIPFAYLLLGEKISKKTLLSLGLSILGILWIVNPWKLANTSRGVIFTLLSAVTF
                     ALYGVLGKAGSSRLGGVALSSFSFLMGSAEMLILILLTRIPRIAAYLSHSGLKLFAAV
                     PVFSGLSWHNMIGLIYIGIFVTGLGYCFYFLAMQETSAATASSVFFIKPALAPVLALI
                     ILHESLAPNTIIGMVLIIAGSGITFIANRKREAAG"
     gene            complement(3480..>4126)
                     /locus_tag="VF531_08725"
     CDS             complement(3480..>4126)
                     /locus_tag="VF531_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7714093.1"
                     /translation="KQGITLQIVEFQDYVQPNLALAQGELDANYFQHIPYLNQFSKDH
                     QLDLTYIAKVHIEPIGVYSNKIKSLDQLPEKALVAIPNDPTNGGRALLLLQRAKLIEL
                     RSGVGLTATELDIAKNPKGLQFKPLEAAELPRALPDVDIAVINTNYALSANLNPTKDA
                     LFIEDSQSPYVNVLAVRTKDKNKAALKKLATALTSKTVKDYIVNTYQGSIVPAF"
BASE COUNT         1007 a         1015 c          910 g         1194 t
ORIGIN      
        1 aagccccttg acgccaattc ctgtcaggga cttttttatt gcagcgacac ggaaaaagtc
       61 gcgtgcgagg atacctgttc gtctgggttt tagggaggaa acattgattc aacaactgtc
      121 cgtaaaagaa ctaaactcag ataaattgaa agactttctg ctgatcaatt tgggcttggc
      181 gctggtggct gccggaattc attttttcaa gataccgaac cattttgcaa ccggtggtgt
      241 tagcggtctg gccatcgtca tccggcattt cttcccaagg atagcggtcg gacccctgat
      301 gctgatcatt aatatattgc ttctaattgc aggctatgca ctgattggcc gaattttcgg
      361 ttctaaaaca gtctattcca gcttcgcact gtccggcatg gtctggttgc taggcaaaat
      421 atacccggtc aatacgcctt ttaccaatga cactatgctg gaattgattc tttccattct
      481 gttacctgcg gtcggttcgg caatcacctt taatctgaac gcatccacgg gtggaacgga
      541 tatcgtggca aaggtgctca acaaactgac cgacatcgac atcggtaagg cccttttatt
      601 ggcggattta gccattaccc tctcagccgg cttcgtttat ggaatacgaa tcggtatgta
      661 ctccttttta gggttggttt tgaaagggtt cgtaattgat tcggtgatcg aagggttaaa
      721 tctgcgcaag caacttgtta tcatcagcga aaaaccggag ctgattcaag aatatatcat
      781 aaacaaccta caccggggag caaccatcca tattgcttac ggtgcgttca ccgatgatga
      841 gaaacacgtg atcaccacgg tagtgaccag ggggcaggcc atcgccttgc gaaaatatat
      901 ccgggaaatt gattcaaaat ccttcattac cattaccaac acctcggaga tcattggaaa
      961 agggtttcga agtatttaga ataagtactt tccagatcga gaatgaatat atgttctgtt
     1021 tatccgtcag tcgattttaa acggttcatc ggtatatttt tagatcggct aatttttccg
     1081 gaagccggaa acagtaatac ggttcctttg aagtccttgg gcatccatct tggcctgcca
     1141 taaaagtttc gatacagtaa caagttgata cccttcgctg atcagctccg gaaccaatat
     1201 ctccagcgct tgaagcgtat tgttgcgatt cccgcccaaa tcatgaaaga gaacaatccc
     1261 ccccggtttc acgaccttta tgatctttcc tgctatcgac cttccgttca ttcccgtcca
     1321 atcccggcta tccaatcccc aagtccagat aatcaccgtg taattcaaac tgcaggcaag
     1381 ttcgtagatt ctttcattac agtatccgct ggttgggcgg tataggcgca tcacttgtcc
     1441 tgtaatttgg taaatagcct ggtgcgagtg ttccaaatcc tcctttaact catcgatagg
     1501 cattccgtat acttcgcggt gttgatacat atgattggcg atttcatgcc cttctctaag
     1561 ttccctctcg atgatttccg gatacttttc cgcctgcgcg ccgacgatga agaaagtccc
     1621 cttggcatga tactgcttta gtagatcgag gatcttattg gtgaatgtag ggctaggccc
     1681 atcgtcaaat gttaatgcaa ttaatttttc atttaccttt gcttccctta cgacacccat
     1741 tttccaggtc aatgaggaat catagaccgg gatcgataag taccggtacg tccccgccaa
     1801 tagcccggca acaactattc ccacgcaaaa tctgcgcaaa agcgcccatc ggataaagta
     1861 tatcttcaaa atatactccc ctataatgtc gtcccataaa tctattcggg atttttagga
     1921 ggtatgattt tcagcgcata cggaagcaac gcttttaagg tacctgtatg attgacaact
     1981 cagagcatct cttatatgat agacacgaaa aatcgcccac gcactctggc cgtcgaccct
     2041 aaccgggctt tctttttatt ttattcatcg agaggtaacc ggaatgaaaa aaggatactg
     2101 gtacattttc ttctccacca tcttgttcag tacgatggag atcgctctta agcttgtaac
     2161 taatgacttt cacccgattc aattgacctt gctccgattc ctcatcggag ggttaatatt
     2221 gttccctttg gcatttcaaa acctgaaaac ccggggagtc tccttaaatg cagcagactt
     2281 tcgttacttt gccattaccg gtttgatttg cgtggtcatc agcatgacct tttatcagtt
     2341 ggccgtctta tacagtaagg catcgaccgt agccatttta ttcagttgta acccggcatt
     2401 cgtaattcct ttcgcctatc tcctgctggg ggagaagatc agtaaaaaga ccctgctatc
     2461 attgggcctc agcatcctgg ggatcctctg gattgtaaac ccctggaagc tggccaatac
     2521 ctcacgagga gttattttta cccttttatc ggcggttacc ttcgcattat acggggtttt
     2581 gggcaaggca ggaagctcca gattgggtgg ggtagcgctg agcagtttca gtttcctgat
     2641 gggaagcgcc gaaatgttga tattgattct cttgacgcga atcccccgga ttgcggcgta
     2701 tctttcccat tccggcctga aactgtttgc agccgtaccg gtcttttcgg gcctttcgtg
     2761 gcataatatg attggcctaa tctatatcgg tatctttgtc accggattgg gatattgctt
     2821 ctacttctta gcaatgcaag aaacctccgc cgccaccgct tcatcggtat tcttcatcaa
     2881 gccggccttg gcgccggtct tggcgttaat catcctccat gaatcattgg ctcccaatac
     2941 tatcatcggc atggtcctaa tcattgccgg ttccggtatc acctttatcg cgaaccgaaa
     3001 aagggaagcc gcaggttgac ctcagaatga atcgtagtta tgatattttc ttattcgatg
     3061 gcttcgaata aatgagtctg ccacctggtg agcggacttg acccggtttt ccggtagtat
     3121 tcgtaagcct tgccgccttt tttcaaaaat tccctccccc ttaaaacttc cctccggaaa
     3181 ccgaacccgt cgtcaccttg gctggacgga ggatttgaca ggacaaaatc aaccccccca
     3241 tgcaacgaat ccatgatgat tcggtccgtc tccaaaaacg gccgatagga atcgaacccg
     3301 cctccttaac attaatggat atgcatcatt gttataatat tcctaacttt tgtacttcca
     3361 cggaaatagc cactctccag cataaaaaag ccaagggtaa atccccttga cttaatgttg
     3421 gtaccacgcg cctcgtgcct catgccttat tgcttgttgc ctatttgcct gctttttccc
     3481 taaaaagccg gcacgatact gccctgatac gtattgacga tataatcctt aactgtcttg
     3541 ctggttaagg cggtagccag ctttttcaaa gccgctttgt tcttgtcttt cgtccgcacc
     3601 gccaggacat tcacgtacgg cgattggcta tcctcgatga acaaagcgtc cttggtcgga
     3661 ttcagattgg ccgacaaggc gtaattggta ttgataacgg cgatatccac atccggaagg
     3721 gcccgcggca gttcggcggc ttccaaaggt ttaaactgca ggccttttgg attcttggca
     3781 atatccaatt ccgtagcggt aagtccgaca cccgaacgga gttctattag tttggcccgt
     3841 tgtaacagaa gtaaggcgcg cccgccattg gtcggatcat tgggaatcgc caccaaagcc
     3901 ttctccggta gttgatcaag ggatttgatt ttatttgagt atacaccgat gggttcaata
     3961 tgtactttgg cgatataggt cagatcaagc tgatgatcct tactgaactg gttcaaatac
     4021 gggatatgtt gaaaatagtt ggcgtccagc tccccttggg cgagggccag attgggttgg
     4081 acgtaatctt gaaactcgac aatctgcagg gtaatcccct gtttgg
//
LOCUS       DASVMB010000304         6855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_701785,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000304 DASVMB010000000
VERSION     DASVMB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701785"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF531_08730"
     CDS             <1..629
                     /locus_tag="VF531_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714094.1"
                     /translation="KLIKPLKTELRRKDIQVQFIQSTLAQRQLAFKNHLSMGRYALMI
                     YANKNGHILALDEVKTGSISYLLDKSGVAKVKTGGNGRGYTGKTPDGKVRKVITASHG
                     PEKKISGEKIPPKRTPEKMSPEIPRPEKVNPKKDGRSDPVIPGKNDLKDKPEEEIIDS
                     EEKVPGPDETVPQDDMKKTNPGSGNPSPQSGGTSSGSKGNSHGTRSGK"
     gene            797..1441
                     /locus_tag="VF531_08735"
     CDS             797..1441
                     /locus_tag="VF531_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714095.1"
                     /translation="MKCSSRVVSFLLILALICTYAIPILADTAETTDPTEPTETAGTS
                     DTTGTDDTTDQTDETTQKETTLPTAQDTGLAQTISKAYGITVTPQDIVDLRVNNSLGY
                     GEIGIMYGMVNASGKTVADILAMRGQDLGWGAIAKSLGLKMSDLMSRNSSVLKAAKLE
                     KEAKVIKDKVDQEQKEQERLSGGQTVGNGQGAAGTGQGKGTGAGKGSGKGKGKG"
     gene            1555..2670
                     /locus_tag="VF531_08740"
     CDS             1555..2670
                     /locus_tag="VF531_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714096.1"
                     /translation="MNDKGIRFGFIGCGQGGGKIADLFSQRGYKAMAINTSPLDLAAL
                     QTIPKKYRVCIGSQLGAGKDPRIGQKAFRSETRKVKAILMKYLAKDVDFIFVISCLGG
                     GTGTGIVADTIELSRETGLLTGCIATIPLISESLEEQKNALKGLDILSDVNPTPFCLI
                     DNESLKAQFGNQSIMEFWPAANKTIVNILDEINQIPKEPSDLFVFDREDYFRLLNTPG
                     CFVPLKIEVTNSEDNDSTTLAASCRQEIIRLLKETGFQGESIRVAALLRCPEDYRVNA
                     ESVELLFQECQSLTNTPTLFRGLYLDPSLQDRIVIYLLLAGLKMPFGRLQEIRNKLQE
                     LSPKIINRINRGVTTSFADDLTEWDGLNPLPASRRSF"
     gene            3041..4042
                     /gene="glpX"
                     /locus_tag="VF531_08745"
     CDS             3041..4042
                     /gene="glpX"
                     /locus_tag="VF531_08745"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX7714097.1"
                     /translation="MIQDKEREVMLDLVRVTESAALMAGRYMGNGNKDLVDGAAVDAI
                     RGMLDLMNIKGTVVIGEGEKDQAPMLYIGEQVGKNDCDFKVDIAVDPIEGTRLVANGL
                     SNALAVVVLAAQGSLASVPAFYMEKIIVGPEAKGYIDLNAPLRENLRVVAACLRKNIR
                     DLTIAILDRPRHQAMIEEIRQLGARVRLITDGDVGAGIATCMDTGIDMVIGIGGATEG
                     VLTAVAIKCLGGDLQARIGPKDETERTKILQHISEHELTRVYTAEDLAHGDSLIFAAT
                     GVTDGDLLKGVHYRGNVATTNSIVMRMRTGTIWRIETTHNLSRKTIRSQKKETEIPV"
     gene            complement(4151..5581)
                     /locus_tag="VF531_08750"
     CDS             complement(4151..5581)
                     /locus_tag="VF531_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7714098.1"
                     /translation="MFKKRLLLLLVLGFVLSGIIGLNPVFAAEQTLGLLYANNYSGVE
                     SIGGSKLVTKVTTRFKENLKKQPAIVFSDDEKTKSLLNGAGLGTYYDCTNLCSTADIL
                     KIGTKIGLNYLAILDINGYNEIKKEKAKKSYQVSLGMRVYNCINGEDNYFSGEGLSDK
                     DRDTAIGNAVEQLINNYLQLVADDPNTGNLRANTTAVIGNQQSKMYHLQDSHHSPSAD
                     NQAMFQTRSAAENEGYRPCPICFPAYSSFFYFDRDLENSLGNEACGTIEYNYRTEYDP
                     EALKRIETVAAPLIADTYRKNFRYHFKLLDTDEVNAFSAPNGYIYLTTGLLNIIESDD
                     ELALVIAHEMGHLERKHAVVRYKQALAVSLFASIFAATVASNNNSRDDAAVLMTVVMA
                     EIIMKGFSQEQEKEADEISLAHLKKIGMDSQVYHALMGKFIDMRSRKIYFIEKLFGTH
                     PTPEKRIENLDKFLQSYQALQTKLQP"
     gene            complement(5762..6193)
                     /locus_tag="VF531_08755"
     CDS             complement(5762..6193)
                     /locus_tag="VF531_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7714099.1"
                     /translation="MQVAFYDIAQVADEEITFVVMVSRYQGEWILVRHQERTTWEIPG
                     GHREADETLEVAARRELFEETGAAEFGLYPVGIYSVSSDGRTSYGKLYFAEVKTLGRL
                     PEFEIAEIILRSDFPQNNLTYPLIQPLLYEKVVDCLKLETR"
     gene            complement(6409..>6855)
                     /locus_tag="VF531_08760"
     CDS             complement(6409..>6855)
                     /locus_tag="VF531_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714100.1"
                     /translation="TYHGGDLRLARLELGASWKHVTNIYGAPFRRKVEKIESPNNPDY
                     VVYLTSWIYPDFEVCFLNSVEKSLPKPEEPGNVFAITVKSNKFQSYRGIRVGDPVGKV
                     VERYGPASTGILDESGSKFYEWQLNYIKFGVSRGKVSNIEVSQNPD"
BASE COUNT         1907 a         1594 c         1571 g         1783 t
ORIGIN      
        1 aaaagttaat caagccgctt aagaccgaac tccggcgaaa agatattcag gtacaattca
       61 tccaatctac actggcccaa agacaactcg cattcaaaaa tcacctttcc atgggtagat
      121 atgcgctgat gatttatgcc aataagaatg gccatatact tgccctggat gaggtcaaaa
      181 cgggaagtat ctcctatctc ttagataaat ccggggtggc aaaggtaaaa accggcggta
      241 acggccgggg ctataccggc aaaactccgg atggcaaagt tcgaaaggtt atcaccgcta
      301 gccacggtcc ggaaaaaaag atttcgggtg agaaaattcc gccaaagaga actccggaaa
      361 aaatgagccc ggaaatcccg cgtccggaaa aggtcaaccc caaaaaagac ggccgaagcg
      421 atccggtcat tcccgggaag aatgatttga aagataaacc cgaagaagaa atcattgatt
      481 cagaagaaaa agtccccggt ccagatgaga cagttccaca agacgatatg aaaaaaacga
      541 atccgggttc aggcaatccg agtccccagt caggcggtac cagttccggt tccaaaggta
      601 atagccatgg aacccgttcg ggtaaatgac atattgtctt ctcttgattg gcaggccttg
      661 tgctgatatg aacatattcc aaaaacaaaa gtgaagggct ttcgcgcgcg tgacacagca
      721 aagagggtgg gaatggttgg gaagacttgc tgttattttg attaaggtat cacataaatc
      781 aaaagaggtg tcttcaatga agtgtagctc cagagtggtt tcctttttat tgatcttggc
      841 cttaatctgt acttatgcaa ttcccatact cgccgatacc gctgaaacaa cagatccaac
      901 cgagccgacc gaaacggctg gaacatctga cacaaccgga accgatgata ccactgatca
      961 aaccgatgag acgacccaaa aagaaacgac cctcccgacc gctcaggata ccggtttggc
     1021 ccaaaccatt tccaaagctt acgggattac tgttactccg caagacatag tggatttgcg
     1081 tgtaaataat tcgttaggat atggcgaaat cgggataatg tacggcatgg tcaatgcctc
     1141 cggaaaaacc gtcgccgata ttttggcaat gcgcggacag gatctgggat ggggtgccat
     1201 tgccaaatcg ttaggcttaa aaatgtcgga tctgatgagt aggaactctt cggttttaaa
     1261 agccgccaag ctggaaaaag aagctaaagt tatcaaagat aaagtggatc aagaacaaaa
     1321 ggaacaagaa agattaagcg gaggccaaac ggtcggtaat ggtcagggtg ctgccggcac
     1381 tggtcaaggt aagggtacgg gtgcaggtaa aggttccggc aaaggaaaag gtaaaggtta
     1441 ataagttacc atcgatacaa aagttatagc catgacccgt tcgggtgatg gttataactt
     1501 tttaccctgt atgcacggcc acgattattg atgttcacgg ggggtaactt cttaatgaat
     1561 gataaaggaa tccggttcgg ctttatcggg tgtggacaag gagggggaaa aattgccgat
     1621 ctcttctccc aaagaggtta taaagccatg gccatcaata cttctccgct agacttggca
     1681 gccctgcaaa ccattcccaa gaaatatcgc gtgtgtatcg gctcccaatt aggtgcgggc
     1741 aaagatcccc gtattggtca aaaagcattc cggagtgaaa ccaggaaggt taaagcaatc
     1801 ttaatgaaat acttagccaa agatgtcgat tttatctttg tcatctcctg tcttggcgga
     1861 gggaccggga ctggaattgt ggcggatacg atcgagttat cccgggagac aggcctcctg
     1921 accggttgca tcgctaccat tcccttgatc agtgaatctc tggaggagca aaaaaacgct
     1981 ctcaaaggcc tggacatttt gagcgatgta aatccaaccc cgttttgtct catcgataat
     2041 gaatccttga aggctcaatt tggaaatcaa tcgattatgg aattttggcc ggcggcgaat
     2101 aagaccattg tcaatattct ggatgaaatc aaccagattc ccaaggaacc ctcggatttg
     2161 tttgtttttg atcgggagga ttatttcaga ttactgaata caccgggatg cttcgtcccg
     2221 ttaaagatcg aagttacaaa ctccgaagat aacgattcca ctacacttgc tgcttcctgc
     2281 cgtcaggaga tcatccgtct cttgaaagaa accggttttc aaggcgaaag tatccgggtg
     2341 gccgctttat taaggtgtcc cgaggattac cgggtaaatg ccgaatccgt ggaattattg
     2401 tttcaggaat gtcaatcgtt aacgaacacg ccaacgctat tccggggctt atatcttgat
     2461 ccgtcgttac aagataggat cgttatctat ctattactgg caggcttgaa aatgccgttt
     2521 ggccggttac aggaaatacg gaataaactc caggagttga gtccgaagat tatcaatcga
     2581 atcaaccggg gtgtcactac cagttttgcg gatgatttaa ccgagtggga cgggctcaat
     2641 cccctgcctg cttccaggcg cagtttttga ttccaaatag ttttcgtacc atgtttcctc
     2701 ttatgttttc ctcaagccca taaccatctc caactcaatc cattcacctt ccgtacaaaa
     2761 caaatccctt atatttgtta acataaggat aataatggac tgatttggca aaaagattat
     2821 aaaatcacca ggattctgat ggtttgatgt ccaattcatc ttttgtccta tcaatatcac
     2881 tttaaccgat gtcggtctct gattttccac cgggaccaaa tgctattttt tatggccttt
     2941 ttagcgtttc aaattactgt ctactctcag cgctaaagtc cttgattgta ttccaaaatt
     3001 taaaaccagc gcaacacatt gacaaagaaa ggacaaaaag atgattcaag ataaggaacg
     3061 cgaagtcatg ttggatctgg tgcgggtcac tgaatccgcc gcattaatgg ccggccggta
     3121 tatggggaac ggcaacaaag atttggtcga cggcgcggcg gtggatgcga tccggggtat
     3181 gctggacttg atgaacataa agggaaccgt ggtcatcggc gaaggcgaaa aggatcaagc
     3241 cccgatgctt tacatcggtg aacaggttgg aaaaaatgat tgcgacttta aagtcgacat
     3301 cgcggtagac ccgatcgaag gtacccgctt ggtggccaat ggtttatcca atgccctggc
     3361 ggtagtggtc ctcgccgccc agggcagtct ggcttcggtc ccggcttttt acatggaaaa
     3421 aatcattgtt ggcccggaag ccaagggata catcgatcta aacgccccgc tccgtgagaa
     3481 cctacgggtg gtggcggctt gtttacgaaa gaatattcgt gatctaacta tcgcaatttt
     3541 ggatcgcccc agacatcaag cgatgattga agagatacgt caattaggag cccgagtccg
     3601 cttgattacg gacggagatg tcggagctgg aatcgccact tgtatggata ccggaatcga
     3661 tatggtcatt ggtatcggag gcgccaccga aggagttttg accgcggtgg caatcaagtg
     3721 tttaggtgga gatttgcaag ccagaattgg gccgaaggat gaaaccgaga ggacgaagat
     3781 ccttcagcat ataagtgaac atgaattaac ccgggtttat accgccgaag acctcgccca
     3841 tggagatagt ttaatcttcg ccgccaccgg ggttacggac ggcgatctgt taaaaggagt
     3901 ccattaccgc ggaaatgttg ccaccaccaa ttcgattgta atgcggatgc ggacggggac
     3961 gatctggcga attgaaacca cgcataacct gagccgaaaa actatccgct ctcaaaagaa
     4021 agagacggaa attccggtat gatttaaaaa gccaacaaat gattttgagt cccctcaaaa
     4081 gtacagtttc gatagaagct ctaaagtggg tttttggggg gactctcctc aaaaggaaat
     4141 gtggataatt ctatggctgt aacttcgtct gtaacgcttg atatgattga agaaatttat
     4201 ccaaattctc aatacgtttt tccggtgtgg gatgggtgcc gaaaagtttt tcgataaagt
     4261 agatcttcct tgatctcata tcaataaatt tacccatcaa ggcgtggtag acctggctgt
     4321 ccatgccgat ctttttcaga tgagccaggg agatctcatc cgcttccttc tcctgctcct
     4381 gagagaaccc tttcataatg atctccgcca ttaccacggt catcaggacg gcggcgtcat
     4441 cacgcgagtt attattgctc gcgacggtag ccgcaaaaat cgacgcaaaa agcgataccg
     4501 ccagggcttg cttatatcgg acaaccgcgt gtttcctttc caaatgcccc atctcgtgtg
     4561 cgataaccaa ggcaagttca tcatcggatt cgatgatatt caacaatcct gtggtgagat
     4621 agatatatcc gttcggtgcg gaaaaggcgt taacctcgtc agtatccagc agtttaaaat
     4681 gatagcggaa atttttacgg tacgtatcgg cgattaaagg agcggcgaca gtttcaattc
     4741 gtttcaaggc ttccggatca tactcggtcc ggtagttata ctcaatggtt ccacaggctt
     4801 cgttacccaa gctgttttca aggtcccggt cgaaatagaa aaagctgctg tatgcaggaa
     4861 aacagatcgg gcagggacgg tacccctcgt tttccgcggc ggaacgtgtc tgaaacatcg
     4921 cctgattgtc ggcagaaggc gaatggtggg aatcctgaag atgatacatt ttactctgtt
     4981 ggtttccaat gacggctgtg gtatttgccc gtagattgcc ggtattcggg tcgtcggcca
     5041 ctaactgcag ataattattg atcaactgct cgacagcgtt gccaatggcc gtatcgcggt
     5101 ccttatcact caatccttca ccggaaaaat aattatcctc accgtttatg cagttataaa
     5161 cccgcatacc caatgatacc tgatagctct ttttggcctt ttccttcttt atttcgttat
     5221 agccattgat atcgaggatt gccaggtagt taagaccgat tttggtcccg attttcaaaa
     5281 tatcggcggt gctgcatagg ttggtgcaat cgtaatatgt accgaggccg gccccgttca
     5341 aaaggctttt ggtcttttca tcatcggaga aaactatcgc gggctgcttt ttgagattct
     5401 ccttgaacct tgtggttacc ttggtcacga gcttggaccc accgatggat tccacaccac
     5461 tgtaattgtt ggcataaagt aaacccaatg tctgctctgc ggcaaagacc ggatttaagc
     5521 caatgatgcc ggataaaaca aaccccagaa ccaaaaggag caaaagccgt tttttaaaca
     5581 atatgaatac cccccaaaga cacgatgcca tctactttca taccctgttt acaccgcaaa
     5641 tatttctata aattttccgg acttcctcct tttaccaaac tagttctgct tcatcacttc
     5701 acctttatcg tttgatggat gataaggaca gcttttatga ccgctgagct attaccactc
     5761 ctcatcgtgt ttctaatttt aagcagtcca ccactttctc atataaaagc ggttgtatca
     5821 ggggataggt aaggttattc tggggaaaat cacttcttaa aattatttct gctatctcaa
     5881 actcgggtaa ccgacctaag gtcttcacct cggcaaaata tagttttcca taggatgttc
     5941 ttccgtcact cgaaaccgaa taaataccaa ccgggtaaag cccgaactct gccgcccccg
     6001 tttcctcaaa gagctcacgg cgggccgcaa cttccagagt ttcatccgct tcccgatgac
     6061 ccccaggaat ttcccaggtc gtccgttcct gatggcggac caggatccat tccccttggt
     6121 atctggatac catcaccaca aaagtgattt cctcatccgc cacctgcgca atatcataaa
     6181 acgcgacttg cattcatact ctcctcgttt tggtttttca ctaccttcaa tcgccgcaat
     6241 tgaagccatt cctattttcg tgtcttttgt gtgtttcgtt gttaataaaa taggcttaca
     6301 gatggaaact tttaaccacg aaaatcacca aatacacgaa atgtctcttc gggcgatatt
     6361 tttcgaaccg aaaaagatga ttctctgtgt cacaaaccat attcatcctc aatccggatt
     6421 ttgacttact tcaatgttcg atactttacc cctcgatacc ccgaacttga tataattcag
     6481 ttgccattca taaaacttcg aaccgctttc atccaaaatg cccgttgatg ccggaccgta
     6541 tctttccact accttaccaa ccggatctcc aactctgatg ccgcgatagg attgaaattt
     6601 gttactcttc acggtaattg caaagacatt acccggttcc tcgggcttcg gaagactctt
     6661 ttcaaccgag tttaaaaaac acacttcaaa atccgggtaa atccaggagg taaggtatac
     6721 aacgtagtcg gggttattcg gtgattcgat cttctcgact ttccgccgga atggtgctcc
     6781 atagatattg gtcacatgct tccatgaggc acccagttcc aaccgggcaa gtctcaaatc
     6841 cccgccatga tatgt
//
LOCUS       DASVMB010000305         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_701960,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000305 DASVMB010000000
VERSION     DASVMB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701960"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF531_08765"
     CDS             complement(<1..428)
                     /locus_tag="VF531_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714101.1"
                     /translation="MRQDLRWKMGLIFILLIIFALLLGPLGKNLFKTDPIRLGLDLKG
                     GIELLLAPDYRIGATQLNKLSNELSAKMTAANIPAPKVEYLGVMENNLYDGLKLTFAN
                     AADIQRARNVGALPTRYNLEAFGETKHLNLTANVAGNVVEL"
     gene            complement(478..891)
                     /locus_tag="VF531_08770"
     CDS             complement(478..891)
                     /locus_tag="VF531_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714102.1"
                     /translation="MKPTLAKGSIVGGCLILLWISMASFISAVSYNDTPEFTSAFRTW
                     LPTASAAMAQVMNWLDYSQPLRDYSGDIALHANSANPSTNFSLIGKRIPPLWGRPLAT
                     KSVPVTLGFFIPAMVLLLFCIFYQSSRTSSDSNPD"
     gene            complement(955..1110)
                     /locus_tag="VF531_08775"
     CDS             complement(955..1110)
                     /locus_tag="VF531_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714103.1"
                     /translation="MSFEIVTVIVVLAIFSLFFYGAYRAEKVVAAEAKLKREAQASKT
                     VGKTRKK"
     gene            complement(1416..1739)
                     /gene="yajC"
                     /locus_tag="VF531_08780"
     CDS             complement(1416..1739)
                     /gene="yajC"
                     /locus_tag="VF531_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7714104.1"
                     /translation="MDWLILAATQPAAAAANGATGAASIWGGLLPMILILVFFWIIII
                     MPQRKQQKQRDAMLKNLKKGDKVITAGGLHGEIVEIDEEDVRLRVADKVELKFTRGSI
                     SRVKS"
     gene            complement(1901..3034)
                     /gene="tgt"
                     /locus_tag="VF531_08785"
     CDS             complement(1901..3034)
                     /gene="tgt"
                     /locus_tag="VF531_08785"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7714105.1"
                     /translation="MAFTFSIQQQSAENRGRLGTITTSHSQIETPVFMPVGTQAAVKA
                     MRVEDLQEIGFRIILGNTYHLYFRPGAEVISRAGGLHSFMRWPGSILTDSGGFQVFSL
                     AKLNKIGEEGVAFKSHIDGSSHLFTPEKVMEIEMALGADIAMAFDVCLPYPSDYEAAR
                     DAMRRTTRWAERCRKAHHRQDQALFGIVQGVTYRDLREESARSLSAMDFPGYAVGGLS
                     VGEPKPIMYEVLDYTVPLLPEDKPRYLMGVGSPDALWEGVARGIDMFDCVLPTRIARH
                     GTAMTSCGKVIIRDAKFTEDFSPLDPNCHCYTCRSYTRAYLRHLFKAGEFLGGYLVTY
                     HNLYFLHQVMAEIREGLRTGTYEERKREFFKRYNAAEEVVLDS"
     gene            complement(3279..>4200)
                     /gene="queA"
                     /locus_tag="VF531_08790"
     CDS             complement(3279..>4200)
                     /gene="queA"
                     /locus_tag="VF531_08790"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7714106.1"
                     /translation="HSLVDRRFYELPQLLAPGDLLVFNDSRVIPARLFAKKVGLNGAR
                     IEVFLLRQTGSSSWECLVWPGKKAKPGVELEFGEGVRAKVTGVVTGGDRIIEFPADLP
                     FREWLLRNGETPLPPYIKQHLDNPERYQTVYSRAEGSVAAPTAGLHFTEELLEGLRKK
                     GINLGFLTLHVGLGTFRPVKVETVEEHTMHSEWYTLSEELVKQIEATKQAGKRVIAVG
                     TTAVRVLESQAGPGGVLRPGSGETAIFIYPGFQFQVIDGLVTNFHLPKSTLMMLVSAL
                     AGREYILEAYRHAVAERYRFFSFGDAMFIQ"
BASE COUNT         1051 a         1161 c         1000 g          988 t
ORIGIN      
        1 agttcgacaa catttccagc gacattcgca gtcaagttca gatgtttggt ttcaccaaac
       61 gcttccagat tataacgggt cggtaaagcc cctacattcc ttgcccgttg aatgtcggcg
      121 gcattggcaa aggtcaactt caacccgtcg tatagattgt tctccatgac ccccaaatat
      181 tccactttcg gtgcgggaat attggcagcc gtcatcttgg ccgacagctc attgcttaat
      241 ttattgagct gcgtggcgcc gatccggtag tccggggcca ggagcaactc gataccgcct
      301 tttaaatcca gacccaaacg aatcgggtcg gttttaaaca ggtttttgcc cagcgggccc
      361 aggagtaaag cgaaaatgat caggagtata aagattaaac ccattttcca gcgtaaatcc
      421 tgtctcaaat ctgtctcctc cttattaatt gattatactt gtccgaaccg ccttccctca
      481 atccggattg ctgtcggagc tggtccggga ggattgataa aagatgcaaa aaagcaataa
      541 caccatggca ggaataaaaa agccaagcgt cactgggacc gacttggttg ccaggggtct
      601 accccatagc ggcgggattc gcttcccaat caagctaaag ttggttgagg gattggctga
      661 attggcatgg agggcgatat caccactgta atcccgtagt ggttgagaat agtctaacca
      721 attcataacc tgggccattg ccgccgacgc cgtgggtaac caggtacgga aagccgaagt
      781 gaactccggg gtgtcattgt atgaaaccgc agagataaaa gaagccatcg agatccaaag
      841 caatatcaaa caaccgccaa cgattgatcc cttggccagc gttggtttca agcctttccc
      901 tccatccatg caaaaacgat acctgttcgg cagccaaagc cgaatccgga cttattactt
      961 cttccgagtc ttacctacgg ttttggaggc ctgagcttcc cgtttgagtt tagcttcagc
     1021 agcgaccact ttttcggccc ggtaggcgcc ataaaaaaag agactgaaga ttgccaatac
     1081 tacgattacg gttacaatct cgaaactcat cgcgttttcc ccctactatt caataattaa
     1141 acatcgaaaa cccgataaat gatacgttta acgggcaata aaccggagtt gtgatatacc
     1201 cggataacct ttctaattat accgattctc cagatgcttg tcaattaaaa ggaagcctcg
     1261 acggtggtca atcaatggat cggattctat attccggttg gtctggaaat caacagaatt
     1321 gaaaataaaa aagtgagacc tgatccggtt cttaccgtga atagtctcac tatcctcaat
     1381 ccctaagagt gtcacgaatc gcgacttacg tgatctcagc tcttcacccg ggaaatcgaa
     1441 ccgcgggtaa atttcagctc gaccttatca gccacacgca acctgacatc ttcctcatcg
     1501 atctcaacga tctcgccatg tagcccgcct gcggttatta ccttatcgcc ttttttcagg
     1561 ttctttagca tcgcatcccg ctgtttctgc tgtttcctct ggggcatgat gatgatgatc
     1621 caaaagaaga ccaggatcaa aatcatcggc agcagtccac cccaaatgct ggccgctccg
     1681 gtagctccat tggcagctgc tgctgccggc tgtgtagccg ctagaatcaa ccaatccata
     1741 tctccacctc ctttaataac aatcattcaa aaaagcctgt tgcccacagc accggttctc
     1801 cgtgaaatgc ctgtacatcg gcgatgaaat aagcaacaat gtaaactgat tcgacatccg
     1861 gtccgttttc cctctattgc cggaaaattg tcatccggtt ttaactgtcg agcactactt
     1921 cctccgcggc attatagcgc ttgaaaaatt ctcgtttgcg ctcctcatac gttccggtcc
     1981 tcaatccttc ccggatctcc gccatcactt gatggagaaa gtacaggtta tgataggtca
     2041 ccagatatcc gccgagaaac tccccggcct tgaaaaggtg gcgcaggtaa gcccgggtat
     2101 aggaacggca agtataacaa tggcaattgg gatcaagcgg cgaaaaatct tcggtgaatt
     2161 tcgcgtccct gataatcacc ttgccgcagg aggtcattgc cgtgccgtgc ctggcgatcc
     2221 gggtcggaag cacgcagtcg aacatatcga tccctcgcgc caccccttcc cacaaggcgt
     2281 cgggtgaacc cacacccatc aaatatcgcg gtttgtcctc gggaaggagg ggcaccgtat
     2341 agtccagcac ttcgtacatg attggcttcg gctccccgac actcaggccg cccacagcat
     2401 aacccgggaa gtccatagcg cttaggcttc gggcgctttc ttcccgtaga tcacgataag
     2461 tgaccccctg cacaatgccg aacaaggctt gatcctggcg atggtgggct ttacggcacc
     2521 gctccgccca acgggtagtg cgccgcatcg catcccgtgc agcctcataa tccgatggat
     2581 acggcaggca tacgtcaaag gccatggcaa tatccgctcc caaggccatc tcgatctcca
     2641 tcaccttctc cggtgtgaaa agatgggatg agccgtcgat atgcgatttg aaagccaccc
     2701 cctcctcgcc gatcttgttt agcttcgcca ggctgaatac ttgaaacccg ccgctgtctg
     2761 tcagtattga acccggccat ctcataaagg agtgcaaacc cccggcgcgg gagataacct
     2821 ccgcgccggg ccggaaatac agatgatagg tattgccgag aatgattcga aagccgatct
     2881 cttgcaggtc ctcaacccgc atcgccttga ccgcagcctg ggttccaacc ggcataaaga
     2941 ccggggtctc gatctgactg tggctggtgg tgatggttcc cagtctgccg cgattttcgg
     3001 cggattgttg ctgaattgaa aaagtgaagg ccataattac ctcttccgat agctttattc
     3061 caatttgtcc aaattatact aacaataggc aataggcaat aggcaatagg caataggcaa
     3121 taggcaatag gcaataggca ataggcaata ggcaatagaa attatcacaa atctcattga
     3181 ttctgtcaac gcatcattga atgaattatc tcaaaatcca actaagtttc ctgttgccca
     3241 ttgcctgttc cctgttgccg cgcgtctcat gagacgcgct attggatgaa cattgcgtct
     3301 ccgaagctga aaaaacggta cctctcggcc accgcgtgtc gatatgcctc aaggatatat
     3361 tcccttccgg ccagcgccga gacgagcatc atcaaggtgg acttgggcag gtgaaagttg
     3421 gtgaccagcc cgtcaatgac ctgaaattga aatcccggat agatgaaaat agctgtttcc
     3481 cctgatccgg gcctcaggac cccgccgggg ccggcttggg attcgagcac ccggaccgcg
     3541 gtagtgccga cggcaatcac ccgtttaccc gcctgtttgg ttgcctcaat ctgtttaacc
     3601 agttcctcgg agagggtgta ccattcggaa tgcatggtgt gttcttctac cgtctccacc
     3661 ttgaccgggc ggaaagttcc caaacccaca tggagagtaa gaaaaccaag gttaatcccc
     3721 ttcttgcgca gtccttccag taattcttcg gtgaaatgga gacccgccgt aggtgcggcc
     3781 accgatcctt cggcgcggga gtagacggtt tgatatcgct cggggttgtc cagatgttgt
     3841 ttgatatagg gaggcaacgg tgtttcgccg tttcgcagca accattcacg aaagggcaga
     3901 tcggccggaa actcaatgat ccggtcgccg ccggttacga ccccggttac cttggcgcga
     3961 accccctcgc caaattcaag ttccacgccg ggtttggctt tcttccccgg ccacaccagg
     4021 cactcccaac tggacgagcc ggtctggcga agcagaaaca cctcaatccg cgcaccgttt
     4081 agaccaacct tcttcgcgaa aagccgggcc gggatgaccc gggagtcatt aaacaccaag
     4141 agatcgccgg gtgccagcaa ctgaggaagt tcgtaaaaac ggcgatccac caaactatga
//
LOCUS       DASVMB010000306         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_182842,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000306 DASVMB010000000
VERSION     DASVMB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182842"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VF531_08795"
     CDS             <1..1344
                     /locus_tag="VF531_08795"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7714107.1"
                     /translation="LGAYDYILKPIDLDYFQELLIKIIREQEQRCKKENEFKALRTRV
                     AANLPLIRTQFIKDLISDGIEPDIIPLKLSELEMTRCSWCQVLLFQLDDFYLITRDLT
                     KEQCRKLESSFNKLFQALDPDTETVVFENQPHEQVMVVWAGTEEALVSKVKTWRHLVR
                     EAVRDLGQYTVTTAIGTPRDCLDKLADSYQEAMSYMQFKFIIGKNREINRQDILEQKG
                     RQEYKAGDLNETELLEAVQLGDKQLVSEGVKTLFQRMLAMGNNSAAAVQMMVSVTYLQ
                     ALKLLGEIGASAEQVFNDPLLVYKGIMAHQTISGMQQELENRLMSVADFISQRKNKRL
                     TQIVNKAKQYIGKNYAKDDLSLEEVAAYVKISPCYFSFTFKQEEGINFIDYLTMIRIE
                     KAKTFLTASAYKSYEVSYLVGYNNPTYFSTIFKKCVGLSPSAYRTRYLQKPADGG"
     gene            1647..2567
                     /locus_tag="VF531_08800"
     CDS             1647..2567
                     /locus_tag="VF531_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7714108.1"
                     /translation="MMCLVAVTATGIMGAPKKLVIGFSQIGAESAWRTAETESIKATA
                     AERGIDLKFSDAQGKQENQIKAIRSFIAQRVMGIIIAPVVETGWEPVLQEAKRARIPV
                     VLVDRGIKVSDPSLYTTLIASDFIFEGRKAAEFIAKKLNGKGNIVELQGTPGASAAVD
                     RQKGFAEFITKYPDLKIIKSQTGDFSRAGGKQVMEAFLKADGDKINAVYAHNDDMALG
                     AIQAIEEYGKKPGTDIIIVSVDGVHAAFEAMIAGKLNCTIECNPLLGPLAFDCIARAI
                     MGEKLPKWIVQKDEVFPQEVAKDVIDFRKY"
BASE COUNT          731 a          631 c          709 g          675 t
ORIGIN      
        1 ctcggcgctt acgattacat cttgaagccg atcgacctcg attattttca agagttactc
       61 atcaaaatca tccgcgagca ggagcagcgc tgcaagaaag agaacgagtt caaggccctt
      121 aggacccggg tggctgcgaa cctgcctttg atccggactc aattcatcaa ggatctgatt
      181 tccgacggaa ttgaacccga cataattccc ctaaagctat cagagctaga gatgacccgt
      241 tgttcctggt gtcaggtgct cctgttccag ctggatgatt tttatctcat cacccgggat
      301 ttgaccaagg agcaatgccg gaagttggaa agttccttca ataaactctt tcaagccctt
      361 gatcccgata cggagacggt ggtttttgag aatcaacccc atgaacaagt gatggtagtc
      421 tgggccggta ctgaggaagc cctggtttcc aaggtgaaga cttggcgtca tcttgtccgt
      481 gaggcagtgc gggacctggg tcaatacacc gtcaccaccg cgattggcac accccgtgat
      541 tgcctggata agcttgccga ttcttaccag gaggccatga gttacatgca gtttaaattc
      601 atcatcggaa agaaccggga gatcaatagg caggacattc tggaacaaaa gggcaggcag
      661 gaatataagg ccggtgattt aaacgagacc gaattgcttg aagcggtaca attgggcgat
      721 aaacaattgg tatccgaagg tgtaaagact ttgttccaga ggatgctcgc catggggaat
      781 aactcagcgg cggcagttca aatgatggtc agcgtaacct atctgcaagc cctgaaatta
      841 ttgggggaga ttggcgcatc ggccgaacag gtctttaatg atccgctttt ggtttataaa
      901 ggaatcatgg cccatcagac catctccgga atgcagcaag aactggagaa ccgcttaatg
      961 tctgtagccg atttcattag tcagcgtaag aataagagac tcacccagat tgtaaataaa
     1021 gccaaacaat atatcgggaa aaattacgcc aaagacgatc tctccctgga agaggtcgct
     1081 gcttatgtaa aaatcagtcc ctgttatttc agttttacct ttaaacagga ggaagggatt
     1141 aatttcatcg attatttgac catgattcgc atcgaaaagg cgaagacgtt tttaacggcc
     1201 tccgcttaca agagctatga ggttagctat ctggtggggt ataacaatcc cacctatttc
     1261 agcactatct tcaagaaatg cgtgggtctt tccccctcag cctaccgtac ccggtatctt
     1321 caaaaaccgg cagacggggg atgagagtga agacatcgga gtaatcactc cttcattttg
     1381 aaccagcctg aactcaccac cgcagaaagt ggtgagtttt tttgttgttc aaaatcgaaa
     1441 aagggttaaa gcttttttaa aaaaacttga ttccgtttca gtaggggaga aggtagtatg
     1501 atcttgtact tcatggaaga aagttttccg gaggaggtgg aaacgagcaa ttctcgcctt
     1561 gagtagggta ctcttgattt tccattttta aaatcaaatg gaggtagagt tggatgaagc
     1621 gaaacactct ctggttcctg ctggttatga tgtgccttgt cgctgtaacg gctaccggta
     1681 taatgggcgc tccgaaaaaa ctggtcatcg gtttctccca gattggcgcc gaaagcgctt
     1741 ggcgcaccgc cgaaaccgaa tccattaaag ccactgccgc tgaacgtgga attgatctca
     1801 aattttccga cgctcaaggt aaacaagaga atcagatcaa ggccattcgt tcttttatcg
     1861 ctcagcgtgt catgggcatc atcatcgccc cggtggttga gaccgggtgg gagccggtac
     1921 tgcaagaggc caaacgggct cggatacctg tagtcttggt ggaccgcggt atcaaagtct
     1981 ccgatccttc cttatacact acgttgatcg cttcggactt catttttgaa ggccggaaag
     2041 ccgccgagtt cattgccaag aagttgaacg gcaaaggcaa catcgtggag ttacagggta
     2101 cccccggtgc cagcgccgcc gttgaccgtc agaagggttt tgcggaattt attaccaagt
     2161 acccggacct gaagattatc aaatctcaaa ccggtgactt ttcccgggcc ggcggcaagc
     2221 aagtaatgga ggcgttccta aaagccgatg gggataagat taacgcggta tacgcccaca
     2281 acgacgacat ggcgctgggg gcgattcagg ccatcgaaga gtatggcaag aaacccggga
     2341 ccgacatcat catcgtctcc gttgacggtg tacatgcggc atttgaagcg atgatcgcag
     2401 gcaaattgaa ttgcaccatc gaatgtaacc cgctcctcgg gccgctggca ttcgactgca
     2461 tcgcccgggc gatcatgggt gaaaagctgc ccaaatggat cgtgcagaag gatgaggtct
     2521 ttccgcagga agtggccaaa gacgtcattg attttcgcaa gtattaatat gtacgcgtga
     2581 tatgaacata agttaagaca tgggactcgg gagccgggtc gggataccgg ctcctccggg
     2641 ttctccaggt caatgctgtt tcggttttta tgaattttaa tcttgatgaa gaggtcagat
     2701 gatgtcgaat actccacttc tttccatccg cgggattacc aagagt
//
LOCUS       DASVMB010000307         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_259709,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000307 DASVMB010000000
VERSION     DASVMB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259709"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF531_08805"
     CDS             <1..268
                     /locus_tag="VF531_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX7714109.1"
                     /translation="AAAVSVSGTIGFVGLVVPHIIRLVIGPGHKRLLPAAALSGGIFL
                     LAADTLARTVLSPQELPVGVITSFLGAPFFIYLLRKSRREVFRY"
     gene            581..>1639
                     /locus_tag="VF531_08810"
     CDS             581..>1639
                     /locus_tag="VF531_08810"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEX7714110.1"
                     /translation="MNNLYKTSENALEVQNLSFKYQDAAILSNIGFTIPKGSFTGIIG
                     PNGSGKTTLLKCLIRLLPPQAGRIALADLDLSKLSIKEIARRVAVVPQQWENRFPFTA
                     GEIVAMGRFPHQRRFRKDSARDREIVRQSMTATHTLHLANRPIFEMSGGEQKRVMIAQ
                     ALAQSPQLLLLDEPTAALDINHQIEISDLLKSLVQKERLTVLTVLHDLNLAAQYCDAL
                     ILLRGGKILAAGPVTEVLTETNLEQVYQTKVRVRMDPMNGKPLITLYSHLRQEPIPDS
                     QTGVRIHLLGGGGSCAGLFEPLNELGFAISAGALNIMDTDWQTAREFEIPLAATAPFS
                     PISPEAHRENLALIDEAAV"
BASE COUNT          392 a          444 c          418 g          385 t
ORIGIN      
        1 ggctgcggcc gtcagtgtca gcggcaccat tggttttgtg ggattggtgg ttccccatat
       61 tatccggttg gtgatcggcc ccgggcataa acgtttactc ccggcggcgg ccttgagcgg
      121 gggtatcttt ctcctggcag ccgatacgct ggcccgtacc gttctatcgc cgcaagagtt
      181 accggtagga gtcatcacct cgttcctcgg cgccccgttc tttatctatc tgctccgtaa
      241 aagccgccgc gaggtatttc ggtactagtt attggttatg tgctgattca ggaagaaaag
      301 cgtgcgtaag tagttgagtg atcaagacac aaagggcgta tttatgttcc gggcttaacc
      361 gggatagaaa actataacga accacgaaac acacgaaatt tcacgaaaaa aggaatcctc
      421 attttatcac tatacccttt caaaaagact attttgggat tatgtttgtt ttcgtgccgt
      481 ttagtgtatt tcgtggttaa aagccgttcc attttacagt caatattttt cgcacttcaa
      541 acttcacaat tcacaattct aagtcggggt ggttggccct atgaataatc tatataaaac
      601 ttctgaaaat gcgctcgaag ttcaaaattt aagtttcaaa tatcaggatg cagccatcct
      661 gtccaacatc ggttttacga ttcccaaagg gagttttacc gggatcatcg gacccaacgg
      721 atccgggaag accaccttgc taaaatgcct gattcggcta ttgccgcccc aggccggccg
      781 gatcgccctg gcggacctcg atctttccaa gctctccatc aaggagattg cccgtagagt
      841 ggcggtggtt cctcaacaat gggagaaccg cttccccttt accgccgggg agatcgtggc
      901 catggggcgt ttcccccacc agaggcgctt caggaaagac tcggcccgcg accgggaaat
      961 cgtgcgccag tccatgaccg ccacccacac ccttcacctg gcgaacagac cgatattcga
     1021 aatgagcggc ggcgaacaaa aacgggtaat gatcgcccaa gccctggctc aatcgccaca
     1081 gctgctactg ctggacgaac ccaccgcagc cttggacatc aatcaccaga tcgagatctc
     1141 cgatttactg aaaagcctgg ttcagaagga gcggctcacg gtattgacgg tactgcacga
     1201 tctaaacctg gcggcccagt attgtgatgc attgatcctg cttcgggggg gaaagattct
     1261 cgcggccgga ccagtgactg aggtattgac cgaaaccaat ctggaacagg tataccaaac
     1321 caaagtccgg gtacgaatgg accctatgaa cgggaaacca ttgatcacac tgtacagcca
     1381 tctgcggcag gaaccgatcc ccgacagtca aaccggggtt cggatccatc tattgggcgg
     1441 gggcggaagt tgcgccgggc tttttgaacc cctaaacgag ttgggttttg ccatctccgc
     1501 cggagccctg aatattatgg ataccgattg gcaaaccgcg cgggaattcg aaatccctct
     1561 tgccgcaacc gctccctttt cgccgatctc tcccgaagcc catcgggaaa atctggcctt
     1621 gatcgacgaa gcggcagtg
//
LOCUS       DASVMB010000308         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_183301,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000308 DASVMB010000000
VERSION     DASVMB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183301"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VF531_08815"
     CDS             <1..1417
                     /locus_tag="VF531_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX7714111.1"
                     /translation="ALQTYSLLTVGDGLVTLIPALLLSTATGILVTRSASEDNLGSDL
                     TKQFFAYPRIYFIVAAVIALFGLMPGMPKLSFFLVAGLVAFLAYSLRQATPVPAMEAG
                     VATGAGGAAGGEAASPEETKKPENVASLLQIDPMELEIGYRLIPLVDPAQGGDLFDRV
                     TMIRRQTALELGMVLPPIRIRDNMQLTPTAYVIKIRGVDVAKGEIVPNYFLAMDPGIV
                     TQPVEGIPTTEPAFGLPALWIAEAQRDAAESAGYTVVDPPSVLATHLTEVIKTHAYEL
                     LGRQEVQSLINNVKEEYSVVVNELIPNLMTIGEIQKVLINLLKEGIPVRNLVSILEAL
                     ADYAPLTKDPEVLTEYVRQSLARQITKMAQSEDGVIRVITLDPNLEQLLLKVQKESQN
                     TPGFTVDPRLIQRIYDKLGELIKSQAATGNQPMVLCSPAVRLVFRKLTERLTAKLMVL
                     SFNEIIPEAEVHSVGVVTVES"
     gene            1414..3081
                     /gene="flhF"
                     /locus_tag="VF531_08820"
     CDS             1414..3081
                     /gene="flhF"
                     /locus_tag="VF531_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX7714112.1"
                     /translation="MRVKRYVADSIQEAISRVKNDLGRNAIILHTKPFKEGGFFGLFA
                     KRRFEVIAAVDENNPDNQKKKVGERTESMQRTAASVSTNLNQGGASGFTQFNIPGSAT
                     DLSMTQPGMNTQGQYGMVQGTTSQSIPGQVMPNSMGQPVPGDIGSEQSRMPVTPFPNQ
                     SQTASLNLNPGVSQFLTPASTNPAVGPMGLSRNQQSQPDGLNAGQAYNREAVPVTPRT
                     GDFLPVRGMTQENPLSLDNTLTQPSETRDLNPASEPVLKSLRAEIAEIKEMIRQVAKP
                     ATGSDDSPEPVGTSIWLRQYLDHLGLEAELIDYLLLQIPANLMDNPDLAEEELVMYCE
                     RLLATKLMFSEPVQTNLPGEQQIVALIGPTGVGKTTTIAKLAANYNLFEGKKVGLITI
                     DTYRIAAVEHLKTYGEIINLPVEVVYTPDDLNQAFQNLHDCDLILIDTAGRSPHNQVM
                     MEELKKFLSHSKIGLILLVVSATTKYQDMTSIANNFSRIAFTHLVFTKLDETDSLGPI
                     INLAWKIRRPISYLTTGQNVPDDIEVAKPDKLIAQLFRQHSPEPAGE"
     gene            3156..>3858
                     /locus_tag="VF531_08825"
     CDS             3156..>3858
                     /locus_tag="VF531_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX7714113.1"
                     /translation="MADQAERLRAIVKENKGVSEPNDVPTRVIAVTSGKGGVGKTNFA
                     VNLGIALSKLGSRALLIDADLGLANVDVILGLIPQYNLGHVILGERKMVDVIVNGPAG
                     LKIVASGSGIARLANLPEQGLDQCLQYLNEIEKFADIMLIDTGAGLSKNVLKFVLAAD
                     EVVIVTTPEPTAITDAYGVIKVVASAEQAKPIWVVVNMAKNETEGVQVMERLATVSQR
                     FLGITLTQVGYIPMDP"
BASE COUNT          992 a          892 c         1004 g          970 t
ORIGIN      
        1 tgcattgcag acctattccc tgttgacagt cggtgatggc ttggtaactt tgataccggc
       61 acttttactc tcaactgcta ccggtatctt agtaacccgt tcagcctcgg aagataatct
      121 cgggtctgac ttgacaaaac agtttttcgc ttatccccga atttacttta tcgtcgccgc
      181 agtaatcgcc cttttcggtt taatgcccgg gatgcccaaa ttatccttct tcttggtagc
      241 gggtttggtg gcctttttgg cttatagctt acgccaggca acaccggtac ctgcgatgga
      301 agcaggtgtt gctaccggag ccggtggcgc ggccggcggc gaggcagcat caccggagga
      361 aacaaagaaa cccgagaatg tggcctcgtt gctgcaaatc gacccaatgg agttggaaat
      421 cggctatcgt ttgattccgc ttgtcgatcc ggcgcagggt ggagatttat tcgatcgtgt
      481 cacgatgatc cggcgccaga ctgccttgga gttaggaatg gttttaccgc cgatccggat
      541 tcgtgataat atgcaactaa cacctaccgc ttacgtgatt aagatccggg gagtggatgt
      601 tgcgaaaggg gagatcgttc ccaactattt tttggcaatg gatcccggaa tcgtcactca
      661 accggtagaa ggcattccca ccaccgagcc tgccttcggg ctaccggccc tctggattgc
      721 cgaggcacaa cgtgacgcgg cggaaagcgc cgggtatact gtggttgacc cgccatcggt
      781 tttggccact catttgaccg aggttattaa aacccatgcc tacgaactcc ttggcaggca
      841 agaggtacag tcgttgatca ataatgttaa ggaagagtac agtgtggtag tgaatgagtt
      901 gatccctaac ttaatgacga tcggagaaat tcaaaaggtt ctgatcaact tattaaaaga
      961 aggaataccc gtgcggaatc tagtctccat cttggaggct ctggcggatt atgcgccgct
     1021 gacgaaggac ccggaggtcc ttaccgaata tgttcgtcaa tctttggctc gtcagatcac
     1081 caaaatggcc caatcggagg atggtgtaat ccgggtgata accttggatc ccaacttaga
     1141 acaactatta ctcaaagtac aaaaggagtc acagaatacc cctggtttta cggtggatcc
     1201 ccgtctgatt cagaggattt acgacaaatt gggtgaactc attaagagtc aggctgctac
     1261 cggcaatcaa ccgatggttc tctgctcgcc ggcagtgcgc ttagtttttc ggaaattgac
     1321 tgaacggctt accgcaaaat tgatggtgct ttcatttaat gagatcattc ctgaggctga
     1381 agttcattcg gtaggggtgg tgactgtgga atcatgagag tcaaacgtta tgttgcagac
     1441 tcgattcagg aggcaatatc cagggtcaag aatgatctgg gccggaatgc catcatttta
     1501 catacgaaac cgtttaagga aggcggcttt ttcgggttgt tcgccaaacg gcgttttgag
     1561 gtgatcgccg cagttgatga gaataaccct gataaccaaa aaaagaaggt tggagagcgg
     1621 actgagagta tgcaacggac agcggcttcc gtttcaacca acttgaatca gggtggagcg
     1681 agcggtttta cgcaatttaa tattcccgga tccgctacgg atctctctat gactcaaccg
     1741 gggatgaata cgcaagggca gtatggaatg gttcagggaa cgacttctca gtcaataccc
     1801 ggacaggtaa tgccgaattc catgggacaa cccgttcccg gggatatcgg atccgagcaa
     1861 tcgcggatgc cagtgacgcc ttttccaaac cagtctcaga ccgcttcact taacttaaac
     1921 cccggcgttt cacagtttct aactcctgct tcgaccaatc cggccgtggg gcccatgggg
     1981 ttatcccgga atcagcaatc ccaaccggat ggtttaaacg cggggcaggc atacaacagg
     2041 gaggctgtcc cggtgacccc gcgaaccgga gattttctac ctgtacgcgg tatgacccag
     2101 gaaaatccgt tgtcgctgga taacaccttg actcagccat ccgaaacccg ggatctcaat
     2161 ccggcaagcg aaccggtttt aaaatcgctt cgtgcggaga tagcagagat caaggagatg
     2221 atccgtcagg tagctaagcc ggcaaccgga tccgatgata gtccggaacc ggtagggact
     2281 tcgatatggc tccgccaata tctggaccat ctgggactcg aagcggaact tatcgattat
     2341 ttattattac agattcctgc aaatctaatg gataacccgg atctggccga ggaagagctg
     2401 gtgatgtatt gcgaacggtt acttgcaacc aagctgatgt tctccgaacc ggttcaaacc
     2461 aatcttcccg gcgagcagca gatcgttgcg ctgatcggac cgaccggtgt cggtaagacc
     2521 acaaccattg ccaagctggc tgcgaattac aatctatttg aaggcaagaa ggtcgggctg
     2581 atcacgattg atacataccg aatcgctgca gtggagcatc ttaaaactta cggagagatc
     2641 atcaatctgc ccgtggaagt cgtttataca ccggatgatt tgaaccaggc ctttcagaat
     2701 ttacatgact gcgatctcat cttaattgat actgcgggtc gaagtcctca taatcaagta
     2761 atgatggaag agctcaagaa attcttatct cattcgaaga taggcctaat cttgctggtg
     2821 gttagcgcta ctactaaata tcaagatatg acgagtattg ccaataattt ttcgcgaatc
     2881 gcctttaccc acctggtctt caccaaattg gacgagaccg attctttagg ccccattatc
     2941 aatctggcat ggaagatccg gcggccaatc tcttatctga cgaccggcca aaatgtgccg
     3001 gatgatattg aagtagctaa acctgacaaa ttgatcgccc aattattccg gcagcactct
     3061 ccggaacctg cgggagaatg aacaatccat aattgatatt atttgacgtt tttagaccaa
     3121 agagattccg ttaattctag atatgaggtt gattcatggc ggaccaggca gaacggttac
     3181 gagcgatcgt caaagagaat aaaggcgtaa gcgaaccgaa tgatgttccc actcgcgtaa
     3241 ttgcagtcac tagcggtaag ggcggggtgg gaaaaaccaa ttttgcagtt aatttgggga
     3301 tagccttatc taagctggga tccagggcct tacttattga cgccgatctg ggtctggcca
     3361 atgtggatgt tattttgggt ttgatccccc aatataattt aggccacgtc attcttgggg
     3421 aacggaaaat ggtggacgtc atcgtcaatg gtcccgcggg attgaagatt gtggccagcg
     3481 gttcggggat cgcccgtctc gccaatctgc cggagcaggg tttggaccag tgtttgcagt
     3541 atcttaatga aattgaaaaa tttgccgata tcatgttaat agatacaggt gcaggtttat
     3601 ctaaaaatgt tttaaaattt gttttagctg ccgatgaagt ggttatagtt acaacaccgg
     3661 agcctactgc catcaccgat gcatacggcg tcatcaaagt ggttgccagt gctgagcagg
     3721 ccaaaccgat atgggtggtt gtcaatatgg caaagaatga gacggaaggg gttcaggtga
     3781 tggaaagact ggcgaccgtt tctcagcgtt ttttgggaat cactctgact caagtggggt
     3841 atattccgat ggacccaa
//
LOCUS       DASVMB010000309         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_260090,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000309 DASVMB010000000
VERSION     DASVMB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260090"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF531_08830"
     CDS             complement(<1..491)
                     /locus_tag="VF531_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7714114.1"
                     /translation="MLEEAERLLRKYYGYPKFREGQAQIIASVLKGSDTFAIMPTGAG
                     KSVCFQIPAMLLPGITLVISPLISLMKDQVDALGTLGIPAGFINSSLSQRKVDERIRE
                     AAAGKYKLLYIAPERLESETFVPLLETLEVALVAVDEAHCVSQWGHDFRPSYRRVGSL
                     VKR"
     gene            complement(756..1595)
                     /locus_tag="VF531_08835"
     CDS             complement(756..1595)
                     /locus_tag="VF531_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714115.1"
                     /translation="MNKGKIPFSKMAVHGVLISGVCLTAIPLIYMITTALRPNAKLYE
                     YPPRLFPDSVTLANFRYILFEQNFGGNFINSIVVALGTVVLAAFVASTLAFVIARFEF
                     PGRNLLFSLIMGTMIIPGMVLILPQFELITWLGLLNSRIGLILVYVSWVVPFSTFLMK
                     GFIEDIPHEFDEAVYIDGGSVGTLFRRIVLPLSGPAIITTCIFNFLTAWEEYPWALTV
                     INDTEKRTLPIAIAGFFGEHNFTQWGYVFAMSLLSLIPVVVIFIFLQRFFISGLSSGA
                     IKG"
     gene            complement(1592..>2018)
                     /locus_tag="VF531_08840"
     CDS             complement(1592..>2018)
                     /locus_tag="VF531_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714116.1"
                     /translation="TAELLGIWKGVGWTMVIFLAALQTIPQELYEAAAMDGCNAWQKF
                     VNITLPSIRRTMLFVVVMLSIGAFNVFTSIKLMTDGGPVHQTEVVLTWMYYKAFDTRK
                     FGYSAALSYIMALIIAAITFIQFKYLQPNETNVSGRLKQ"
BASE COUNT          613 a          510 c          410 g          485 t
ORIGIN      
        1 cagcgtttta ccagagaacc gacccgacga taactgggac ggaaatcatg tccccattgc
       61 gagacacagt gagcctcatc cacagccacc aaagcaactt caagggtttc aagcagaggg
      121 acaaaggtct ccgattccaa tcgctccgga gcaatataca atagcttata ctttcctgcg
      181 gccgcttctc gaatccgctc atcgactttt cgttgggaca gggagctgtt gataaagccg
      241 gccgggatgc caagagtccc taaggcatcc acctgatcct tcattagaga gatcaggggc
      301 gaaatgacca aggtaatgcc gggcaaaagc atggccggaa tctgaaagca aaccgactta
      361 ccggctccgg tgggcataat ggcaaaggta tcggatcctt tcaaaacact agcgatgatt
      421 tgggcctggc cctcacggaa cttgggatat ccataatact tccgcaacag ccgctctgcc
      481 tcttccagca tgatgccacc ccccggttcc agaagattca ttgggtacgg cgtaatgctt
      541 cgctaacctc cgcgtaactc ctgccaaccg acgggaacaa ttttgtgcat gtgagtccta
      601 cgagtatatg aaaccctgca gattttttac tccgttcgcc ttccgaattt tatttgacaa
      661 ttatttattt ctcttttacc tttccgtgta catgaaaaac gggagaatca agacatcctc
      721 ccgctaaata ttatttggcc attttcatta agtgattatc ccttaatagc ccccgacgat
      781 aagccgctga taaaaaagcg ctgcaaaaaa atgaatatga cgactacagg aatcaaagag
      841 agtaacgaca tggcaaaaac atatccccat tgcgtgaaat tatgttctcc aaaaaagccg
      901 gcgatcgcaa tcggtaaggt ccttttttcg gtatcattaa taactgtcaa tgcccaggga
      961 tactcttccc acgcagttaa aaaattaaaa atgcaagttg tgataatcgc cgggccggat
     1021 aacggtaaaa caatccggcg gaaaagagtg ccgacacttc caccgtcaat ataaaccgcc
     1081 tcatcgaact catgcgggat atcctcgatg aatcctttca ttaaaaaggt tgagaaaggt
     1141 acaacccagg atacatatac caggatcaat ccaattctac tgtttaacaa gcccaaccag
     1201 gtaatgagct caaattgggg caagatcaaa accattccgg gaatgatcat tgtacccata
     1261 atcaaggaaa aaagaaggtt cctgcccgga aactcaaacc gcgcaattac aaaagctaaa
     1321 gttgatgcca cgaatgctgc taaaacaact gttcctaacg caactacgat actgtttata
     1381 aaattgcccc caaaattttg ctcaaacaat atataccgga aattagccaa agttacacta
     1441 tcgggaaata accgcggggg atactcatat aatttcgcat tcggtcgtaa agccgttgta
     1501 atcatataaa tcaacggaat tgccgtcagg cacactccgg agatcaaaac cccatgaacc
     1561 gccattttac tgaatggaat ctttcctttg ttcattgttt taacctcccg gaaacgttcg
     1621 tctcattcgg ctgtaagtat ttgaattgaa taaacgtaat cgccgcaatg attagcgcca
     1681 tgatatagga cagagccgcc gaataaccga acttcctggt atcgaacgcc ttataataca
     1741 tccaggttaa aaccacctcg gtctgatgga cgggaccccc atcggtcatc agtttaatgg
     1801 aagtaaatac gttaaaggcc ccgatactca acatgactac tacaaaaagc attgtccgtc
     1861 tgatcgacgg cagtgtgata ttcacaaact tttgccatgc attgcagcca tccattgccg
     1921 ctgcctcata caattcctga ggaatggttt gaagcgcagc caaaaagatg accattgtcc
     1981 agccgactcc cttccagatt ccgagcaatt cggcagtt
//
LOCUS       DASVMB010000310         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_702845,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000310 DASVMB010000000
VERSION     DASVMB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702845"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF531_08845"
     CDS             complement(<1..212)
                     /locus_tag="VF531_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714117.1"
                     /translation="MRKTLPYLFILCLFLTVPVFADDVFTKYNFVPGEKIIAFEDLST
                     AKIGDIPVGTFKLSGAAEVVLIDNQR"
     gene            complement(289..1275)
                     /locus_tag="VF531_08850"
     CDS             complement(289..1275)
                     /locus_tag="VF531_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714118.1"
                     /translation="MKVHVFILLLALTIPPVAVWAAPGPVAETAVPIYGGAIEDKNAA
                     GQFDQEDPVTMFADTELILRSKSNKVYSTNSPVEDVFRFYLQKLGAESNVTRIELEQL
                     FPGKSTPVEYEVIFYESEFRDLVEPNSGTVIRSGKMVRNSLASRQPYQPEKWIKEATF
                     LWEVREENEDLTGYAVIIYDKSFSEDYKTYKTKTWIVVTRQTYQSEEDSMNQKIDEDS
                     AATLKTMKQPTERELGAPIYPGSIFNKEASAGMSLNEDQYYIFLSNDLPEKVASFYQQ
                     KLGKQALNNQGKYLIALKGNLPWPDNAISIEPNTLGGAAKTMITFKLSPPTE"
     gene            complement(1438..>2405)
                     /locus_tag="VF531_08855"
     CDS             complement(1438..>2405)
                     /locus_tag="VF531_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7714119.1"
                     /translation="LMALLGERSLSILSILAVHKEPAVRLRTVIPLAEIGTLKTKALM
                     KDLMNDGDHDVAETAKHLAGFDNPSAIQSCVISDADNPLLEFKTLGQFRVFVDGVEIN
                     PESWRTVKVRDLMAYLIHNTEPVNTFQIIEDLWPQFDQKKAATNFHTTLYQLRQRLKQ
                     VGCDNPVEHGNRHYFIQPVSYRADYQRYENLVKAHLQIGVSKENAANLEEALKLYCGN
                     YLADLDYEWVIPKQEYLKRLYFDACMALSDYYLELQEFNLAINHLQSVQANNPFNEDV
                     YCRLMTAYAGKGDRAAVRKEFERLKEELGLEPSGNTVAIYRRLYH"
BASE COUNT          624 a          553 c          512 g          716 t
ORIGIN      
        1 cagcgttgat tgtcgattag aactacttcg gctgctccac ttagtttaaa tgtcccaacg
       61 ggaatgtctc cgattttggc tgtggagagg tcttcaaaag caatgatttt ttcaccgggt
      121 acaaagttat atttggtgaa aacatcatct gcaaaaacgg gtacggttaa gaataaacat
      181 aagatgaata agtacggtaa cgttttacgc atgagttttc ccccttgaga tgttttccga
      241 agtgaaaatg caatgatcca atatcccatt cttacggatg atcatcttct actcggttgg
      301 tggagacagc ttaaaggtga tcatcgtctt cgccgctcca cccagggtat tcggctcgat
      361 agatattgca ttatccggcc aaggcaagtt acctttcaaa gcgattaagt attttccttg
      421 gttattgagg gcttgtttcc ccagtttttg ctggtagaaa cttgccacct tttccggaag
      481 gtcatttgat aaaaagatat aatactggtc ttcatttaaa gacatccccg cggatgcttc
      541 cttattaaat atagaaccgg gataaatcgg cgcacccagc tcccgctcgg tcggctgttt
      601 catcgtcttt agcgtggcgg cggaatcttc atcgatcttt tgattcatgc tgtcttcttc
      661 cgattgatag gtttgccggg ttactacaat ccaggttttc gtcttgtagg ttttataatc
      721 ttcggaaaat gatttatcgt agattattac ggcgtaaccc gtcaagtctt cattttcttc
      781 ccgtacttcc caaagaaagg tggcctcttt gatccatttt tcgggttggt agggctgccg
      841 agaggcgagc gagtttctga ccattttccc ggagcgtatg accgtcccgg aattcggttc
      901 caccaaatcc ctaaattccg attcgtagaa gataacctca tactctaccg gggttgattt
      961 ccccggaaac aattgttcaa gctcgatccg tgttacattt gattcggctc ccaatttttg
     1021 cagatagaac cggaacacgt cttcaaccgg ggaatttgta ctgtatacct tgtttgattt
     1081 cgatcgcagg attaattcgg tatccgcaaa catggtgacg gggtcctctt gatcaaactg
     1141 gcccgcggca ttcttatctt cgatagcccc gccatatatt ggtacggctg tttcagcgac
     1201 tggtcccggg gccgcccaaa ccgctaccgg aggaatagtc aaggccagta acagaataaa
     1261 aacatgaacc ttcataatct catctcccag ctgtcatttt atcctcgcta acgggtgttt
     1321 taaaaaccag cctgacttga ttataattaa tcatctctga acaaaaaatg aacagctgac
     1381 cggtatatat gaaaaggggg tttgtcggca agataggggt ggagaaatag gagcggatta
     1441 gtgatacagt cggcgatata tcgctactgt attcccggag ggttcaagtc ccagttcctc
     1501 tttcaatcgt tcaaattctt tccgaacggc cgcccgatct cccttccctg cataagcagt
     1561 cattaatctg caataaacat cttcattaaa gggattattc gcttgcaccg actgaaggtg
     1621 attgatggcc aggttaaatt cttgcaattc caaatagtaa tcggatagtg ccatacaggc
     1681 atcaaagtaa agtcgcttta agtattcctg cttcgggata acccattcgt aatcgagatc
     1741 cgccaaataa tttccgcaat ataactttaa agcctcttca agattggcgg cgttctcctt
     1801 ggaaacccca atttggagat gagctttaac taaattctca taacgttggt aatccgcacg
     1861 gtaactgacc ggttgaataa aataatggcg gttgccatgt tcaaccggat tatcacatcc
     1921 tacttgtttc aatctttgcc gtaattgata caaggtggtg tgaaaatttg ttgccgcctt
     1981 tttttgatca aactggggcc ataaatcttc aataatctga aaggtattta ccggttcggt
     2041 gttatgaatc aaatatgcca taagatctct gacttttacc gtccgccagc tttcgggatt
     2101 aatctccaca ccgtcaacaa atacccggaa ctgtcccaat gtcttgaact ccaacaacgg
     2161 gttgtctgca tctgaaatca cgcaggattg aatcgcggac ggattgtcga aaccagccaa
     2221 atgttttgct gtctcggcaa cgtcatgatc accatcattc attaagtctt tcattagggc
     2281 tttggtcttt agggtcccaa tttccgccag aggtatcacc gttcttaacc tgacggccgg
     2341 ttctttatga actgccaata tcgataaaat cgataaggag cgttcaccca aaagcgccat
     2401 caggt
//
LOCUS       DASVMB010000311         5425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_553452,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000311 DASVMB010000000
VERSION     DASVMB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553452"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1480
                     /locus_tag="VF531_08860"
     CDS             167..1480
                     /locus_tag="VF531_08860"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7714120.1"
                     /translation="MSDVQATTTMEKIVSLAKRRGYVFQSSEIYGGLNSCWDYGPLGV
                     ELKNNVKRAWWRKVIQERDDMVGLDASILMHPKVWEASGHVAGFTDPMVDCKSCKQRF
                     RADHLEGDRCPACGGELTEARQFNLMFKTFMGPVEDSASVVYLRPETAQGIFVNFNNV
                     LTTTRMKLPFGIGQIGKSFRNEITPGNFTFRTREFEQMEIEYFVKPGTDEQIHQEWLK
                     QRFQWYLDLGITKERLRLRQHDKDELAHYAKDCYDIEYFFPMGWSELEGIANRTDFDL
                     KRHQEYSGQRMDYFDQEANEHFVPYVIEPSAGADRGTLAFLLDAYHEEPDKDEIRVVL
                     KLHKALAPYKVAVLPLSKKEPLVELAKGIERQLRKHWMIDYDDSKAIGRRYRRQDEIG
                     TPYCVTVDFNSIEDQQVTVRDRDTMAQERLPIAELTAYLREKLDW"
     gene            1782..2828
                     /locus_tag="VF531_08865"
     CDS             1782..2828
                     /locus_tag="VF531_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714121.1"
                     /translation="MERFVLVRGKKVTLDDVATAVGLSRFLVCRALTGQPGVNPETRQ
                     RIQETATRMGYRGRKTAPGPNHQNLSSVALLIDEAEANRVFWGKIISGIEAAARNAGC
                     DLLLRAVTREEVERGEIPALILEGRIKGVLITGNFIPEYVARFERIDCPVVLVDNYIP
                     VTTHDAVLVADWEGSYQVTRYMIESGHRKLGYAGQTCGHWSWSQRFLGFLSALEDNGL
                     KYDPRFAIGSKCGTEVWSSEFMNREVGRMKEMPTVWICNNDLTANYLIKSLNDLGLAV
                     PEDISVTGFDNLAPDDSPRVPDLTTIRVFGSEIGKAAFDQLVWRLGNREAPPRRVMVG
                     VRLIPGLTTASPHA"
     gene            3375..4418
                     /locus_tag="VF531_08870"
     CDS             3375..4418
                     /locus_tag="VF531_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEX7714122.1"
                     /translation="MANLSGSKTCSPSQSGDAISSSISVVGPALPNIPWENRPANCNE
                     VVWRFSKNPIIPRNLIPSANSIFNSAVIPFQGEFAGVFRCDDRRRQMELHSGHSKDGL
                     HWELNDKPITFICDDSEIGHFVYGYDPRVCWIEDRYYVTWCNGYHGPTIGVGYTYDFE
                     KFYQLENAFLPFNRNGVLFPRKINGKYAMLSRPSDNGHTPFGDIFYSESPDMCYWGRH
                     RHVMSTAGGWQATKIGAGPVPIETNEGWLLIYHGVLTSCNGFVYSFGAALLDPDQPWK
                     VIYRTAPYILSPQMPYECVGDVPNVAFPCAALCDPASGRIAIYYGGADTVTCLAFAKL
                     DELVEFIKKNSMI"
     gene            4686..>5425
                     /locus_tag="VF531_08875"
     CDS             4686..>5425
                     /locus_tag="VF531_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7714123.1"
                     /translation="MKKFVLVLLMISLVVTIGAFSANVLGENQIKGKVIFTTQRTDKI
                     DKELPALAAEFAKAYPGAQIEFEGIKDAENLLKTRMATHSLADVTLVIDQLVRTDYPK
                     FFVPIDDLGFTEKNIIDYQRGINPAEKKLYTVTHAANYDGFVYNKKVFRECGITKVPT
                     TVAELLAACEKIKAKGIIPVASNYKDLWPLNDYCMASGYFNTGDPDYYNKLAKKDKFL
                     VDDGGPLTTFKILRTLNQKGYLEPDLAST"
BASE COUNT         1353 a         1260 c         1377 g         1435 t
ORIGIN      
        1 gctattacgg cttgctgatt ctatgcctgt tgaccattgc ggcagttttt gaggtaacgg
       61 taagtccgct cctgatgcac caatgattat tgacatgcag acatctttac cggtgagcgc
      121 gtttattctt gggcgataat aattctcatg aggaggtttt catcttatgt ccgatgttca
      181 ggcgaccact accatggaga agatcgtatc actggcgaaa cgccgcggtt atgtttttca
      241 gtcgagtgaa atttacggcg gattgaacag ttgctgggat tacggcccat tgggggtgga
      301 gttgaaaaac aatgtcaagc gcgcctggtg gcgaaaggta attcaggagc gcgatgatat
      361 ggtgggtttg gatgccagta ttttgatgca tcccaaggtt tgggaggcca gcggccacgt
      421 ggctggtttc accgatccga tggtggactg taaatcctgt aaacaaaggt tccgcgccga
      481 tcatcttgag ggtgatcgtt gtccggcctg cggcggggag ttgaccgaag cccggcagtt
      541 caacctgatg ttcaagacct tcatggggcc ggtggaggac agcgcctctg ttgtctatct
      601 ccgtcccgaa accgcacagg gaattttcgt caacttcaac aatgttttga ctactacccg
      661 aatgaagctg ccattcggga tcgggcagat cggcaagtca ttccggaatg agatcactcc
      721 aggcaacttt actttccgga cccgcgaatt cgaacaaatg gagatcgagt atttcgtaaa
      781 accgggcact gatgaacaga tccatcagga atggctgaaa cagcgttttc agtggtatct
      841 cgatctgggt ataaccaagg aacggctccg tctgcgtcag catgacaagg atgagttggc
      901 tcactatgcc aaggattgtt acgacattga gtatttcttc ccgatgggct ggtcggagtt
      961 ggagggaatt gccaaccgga ccgattttga tctcaaacgc catcaggaat acagcggtca
     1021 acggatggac tactttgatc aggaagccaa cgaacacttt gtgccttatg tcatcgaacc
     1081 ttctgcgggt gccgaccgcg gcactctggc ctttttactc gacgcctatc atgaggagcc
     1141 tgataaagac gagatccggg tggttttgaa attgcataag gccttggccc cgtataaagt
     1201 ggcggtatta cccctctcca agaaagaacc tttggttgaa ttggcgaagg gaattgagag
     1261 gcaactgcga aaacattgga tgattgatta tgacgattct aaagcgatcg gccggcgcta
     1321 ccgccgccag gatgagatcg gcacgcctta ttgtgtaact gttgacttca acagcattga
     1381 ggatcagcag gtaactgtgc gcgaccggga caccatggcc caggaacggt tgcccatcgc
     1441 cgagttgact gcctaccttc gggagaaact ggactggtaa ttttcggatg atacctcctt
     1501 ccggttttca agttgctcaa agtgtatacc aggcaattga tcgcggatgt cccgaaactt
     1561 tatacgcctt agttttcctg aaaaatagcc tgccggtatc ctaccggcgg gctgttttta
     1621 ttcctccgtt gaccgcgctt gacaaaaaaa tgatttatct ttaaagttat attgactgca
     1681 atttatgaac gaattgaaaa cattcaatga actgaatagc gatccgtcgc tcatcagcag
     1741 ttactcggat ttgattccta tgtttcggaa ttgactggga aatggagcga tttgttttgg
     1801 taaggggaaa aaaagtaact ttagatgatg ttgcgactgc ggttggtcta agccgttttc
     1861 tagtatgccg ggccttgacc ggtcaaccgg gagtcaatcc cgagacgcgg cagaggattc
     1921 aggagaccgc aacccgcatg ggctaccggg gccggaagac tgccccgggg ccaaatcatc
     1981 aaaacctttc ttcggtagcg ctgctcattg acgaagcaga agccaaccgg gtcttctggg
     2041 gcaagatcat atccgggatt gaggctgcag cccgcaatgc cggttgcgat ctcctgttac
     2101 gcgccgtcac tcgcgaggaa gtggaacggg gcgagattcc ggcgttgatc ctggagggcc
     2161 gaatcaaggg ggtgctcatc accggcaatt tcatcccgga atatgtagcc cgcttcgaac
     2221 ggatcgactg cccggtggtg ctggtggata actatatccc ggtaaccacc catgatgcgg
     2281 tgttggtcgc ggactgggaa ggaagttatc aagtaacccg gtatatgatt gaatccggcc
     2341 accgcaagct gggatatgcc ggacagactt gcggccattg gagttggtcc caacgcttcc
     2401 tcggtttttt aagcgctttg gaagataatg ggctgaaata cgatcctcgt ttcgccatcg
     2461 gatcgaaatg cgggactgag gtttggagtt cggagtttat gaaccgggaa gtggggcgga
     2521 tgaaggagat gcccaccgtc tggatttgta acaatgatct taccgccaat tacctgatca
     2581 agtccttgaa cgatctggga ctcgcggttc ctgaggatat atcggtaacc ggattcgaca
     2641 atctggcgcc ggatgactcg ccgcgcgtac cggatctgac tacgatacgg gtttttggtt
     2701 cagaaatcgg aaaggcggcc tttgatcaat tggtctggcg tttggggaat cgtgaagctc
     2761 cgccgcgccg ggtgatggtg ggagtgcgac taatacccgg tctcactacg gcttcgcccc
     2821 atgcttagag ttgacccata ttttattcat tgtgaaccta ttagtacgaa caccggagcg
     2881 atgtgtcatt ttattggaca catcgctttt ttgttgctct cccttctttc cgccaactta
     2941 cttaacctcc aggtacaact tcttgcaatg ttttgcaata tattgcacaa attgactgca
     3001 acaatttaca atctcttaca tcgtttattg tctacaatcc ccttttaatt tgtttttaac
     3061 ctttttccga tttgccggcc ggattgagtt gcttattctc cacgctcttt ggtgtttcaa
     3121 tgattgacaa gatgttacaa ccatgttaaa gtaattgttg atgacaataa atgcaattat
     3181 ttaatcatca tcgccaaata ccttccgtgt tttccctgag taaatccaga agaaaaaaca
     3241 tgttaataat ggatgggcta attgccggca attcatcaac ctgattcacc ggactcttgc
     3301 gccttggaaa tacgccggtt acttaggggt tttaatcgat cattcttata ttttatttgg
     3361 agaggggatt catcatggct aatctttccg gtagtaaaac ttgttctcca tcccaatccg
     3421 gggatgcaat ttcatcaagc atttcagtag tgggtccggc tttacccaat attccctggg
     3481 aaaaccgtcc cgccaattgt aatgaagtgg tatggcgttt ttccaaaaac cccatcattc
     3541 ccaggaactt gattccctcg gccaatagca tctttaacag cgccgttata ccgtttcaag
     3601 gggagtttgc cggagtattc cgctgtgatg atcgccgccg ccagatggaa ttacatagcg
     3661 gtcatagcaa ggacggactc cactgggaac tcaatgacaa gccgatcacc tttatttgtg
     3721 acgattctga aatcggacat ttcgtatacg gatatgatcc ccgggtctgt tggatcgagg
     3781 accgttatta cgtcacctgg tgcaacgggt atcacgggcc gacgatcggt gtgggataca
     3841 cctatgattt tgagaagttt tatcagcttg aaaatgcttt cttgcctttt aaccggaatg
     3901 gggtgctctt tccccggaaa atcaacggca agtatgccat gttgagccgg cccagtgata
     3961 atgggcacac cccgtttgga gatatctttt atagtgagag tcctgatatg tgttattggg
     4021 gccgtcaccg tcatgtcatg tccacagccg gtggctggca agccacaaag atcggggccg
     4081 ggccggtacc aattgagacc aatgagggtt ggctgctcat ctaccacgga gtattaacct
     4141 cttgcaatgg ttttgtatat agcttcggtg ctgcactgct cgatccggac cagccttgga
     4201 aagtgatcta ccgcactgct ccttatatcc tttcacctca aatgccctat gaatgcgtag
     4261 gagacgttcc caatgtggca tttccgtgcg ccgccttgtg cgacccagcc agtggaagaa
     4321 tagccatcta ttatggaggg gcagataccg ttacctgtct ggcctttgct aaacttgacg
     4381 aattagtgga gtttattaaa aagaattcca tgatttaaag atggttggaa ttaaggttca
     4441 aagtatcggg ccgctgttaa tcatgtggcg tcatacataa ttatattgca accatcaacg
     4501 cagattttac aaaataggag atgaaaaagc gctaaatggt aaaaacgacc tgaaacaagg
     4561 tcgttttttt tatcaattca tttacatcca atgataaatg cttgaaatat cgagctaagg
     4621 ggggatgcgc ggcgagaaaa acttccggta tccagtaata agagaattca gaaggggtgt
     4681 tgagtttgaa gaaatttgtt ctcgtgttgt tgatgatttc actcgttgta acgatcggcg
     4741 ctttcagcgc caatgtcctc ggtgagaacc agattaaagg aaaagtgatt tttaccactc
     4801 aacggaccga taaaatcgac aaagaactcc cggcattggc cgccgagttc gctaaggcct
     4861 atcccggcgc tcagattgag tttgagggca tcaaggatgc cgagaacctc ttaaagacca
     4921 gaatggcaac tcattcgctg gcggatgtga ccctcgtcat cgatcaactg gtccggacgg
     4981 actatccgaa attctttgtc cccattgatg atcttgggtt taccgaaaag aatatcatcg
     5041 attaccaacg tggaataaac cccgcagaaa aaaaacttta taccgtaact catgcagcta
     5101 attacgacgg ctttgtctac aacaaaaagg tcttccgtga atgcggaatc accaaggttc
     5161 cgaccactgt ggcggagctg ttggctgcct gtgaaaagat caaagccaag gggattatcc
     5221 cggtagcgtc caactacaaa gacctctggc cgcttaacga ctactgcatg gcctccggtt
     5281 atttcaacac cggtgatccc gattactaca acaagcttgc caaaaaggac aaatttctgg
     5341 ttgacgacgg cggtccgttg accacgttca aaatcttacg gaccctgaac caaaaaggtt
     5401 acctggaacc ggacctcgct tccac
//
LOCUS       DASVMB010000312         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_479934,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000312 DASVMB010000000
VERSION     DASVMB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479934"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF531_08880"
     CDS             complement(<1..559)
                     /locus_tag="VF531_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714124.1"
                     /translation="MAKLNKIMIIYANSGAGHRRAAEALYSTMQETFPAAEIKILDVL
                     DYTNPVFKKSYPQSYLFLVRYCPLLWGFGYYIIDNPYIDPIVRHFRRAANSRQAKGFL
                     NLVASEQPDLIITTHFLPNELLSIMKERKRLKSFVVTCITDYHPHIIWMDSGVDLYMT
                     PTPEMTPKLKSWGISERKVLPLGLPI"
     gene            complement(826..1542)
                     /locus_tag="VF531_08885"
     CDS             complement(826..1542)
                     /locus_tag="VF531_08885"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7714125.1"
                     /translation="MRLDRYLSNMGRGSRSQVKRLIRSGQVTVNSDTCRNEAMAVDPG
                     SDEVTCAGEIVVYREFIYLMLNKPAGVLTATEDGRGRTVLDLIGPEIPKRGLFPVGRL
                     DKDTEGLLLLTNHGELGHRLLAPQNHVPKRYYARVTGAAGTVDQQAFATGVELDDGYR
                     TLPAGLELISSGEVSEVIVEIHEGKFHQIKRMFQTLGKRVLYLKRLTMGPLCLDESLT
                     SGSYRELTADEIKAINDLDT"
     gene            complement(1571..>2949)
                     /locus_tag="VF531_08890"
     CDS             complement(1571..>2949)
                     /locus_tag="VF531_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7714126.1"
                     /translation="VREYLVKNLGFSEDELRWGGYKIYTTLDLNYQRRAELALQQIPG
                     FSAKIQPQAALVTLDPTNGGILAMVGGRDYARSQLNRSITAYRQPGSAVKPFVYATAF
                     EKGAYNAASVFEDKPLSITLENGTVWTPTNYDNTYNGRMTLREALRQSVNSVAVQLLQ
                     AVGVDAVAEQMEKMGINSLVKSGSNNDLNLAPLALGGLTKGVTPMELALAYTPFANQG
                     NLVKPFAVRKVLDGHGNLLKQFAPASPQPALSPQTAFIMTMLMEDVIDRGTGVRAKLP
                     ERPAAGKTGTTSDYTNAWFVGYTPDLLTTVWIGNDRQGVPMRYKEGNIGSASAAEIWG
                     NYMRAITVGRPVTQFTQPSGIVWADVNPDTGQAVPGWLAGNSYKEVFAEQNVPQSAAY
                     KLWHWLFPGQKEEPQTEAPGTDGADTPNPGTPATNRAEPVVSPSPAPGNQTGTDSVQP
                     PVPAVSQP"
BASE COUNT          714 a          819 c          723 g          693 t
ORIGIN      
        1 gaatcgggag tcccagaggg aggacttttc gttccgaaat cccccagctt ttaagctttg
       61 gggtcatctc gggggtcgga gtcatatata gatccacccc gctatccatc cagatgatgt
      121 gcggatggta atcggtgatg caggtcacca cgaatgactt caaccgtttc cgttctttca
      181 ttatcgaaag cagttcatta ggcaggaaat gagtagtgat aatcaaatca ggctgttccg
      241 aggcgaccag atttaagaaa cccttggcct ggcgggaatt ggcggcacgg cgaaaatgcc
      301 ggacgatcgg atcaatgtaa ggattatcaa tgatatagta tccgaaaccc cacaacaacg
      361 ggcaataccg gactaaaaac aaataagatt ggggatagga tttcttaaag accgggttgg
      421 tataatccaa cacatcaagg attttaatct ctgcggcagg aaatgtttcc tgcatcgtgg
      481 agtataaggc ctccgcggcg cggcggtgtc cggctccgct gttggcataa atgatcataa
      541 tcttattcag tttcgccatg cctcgtttct taatttaact gtcccggtgc aaatcgccga
      601 taccaccgaa cgccaatcca aaacgggttt ataagaggaa cgcaatggag atttaatgac
      661 cagagaaaga aagatggata aacccgaata taaacatgtt gaaaaaataa atccggaacg
      721 tgaccggcgg ttaaaatcta ccgtacagag acgttccgtg agacaaaaac attatgttac
      781 ttttaaactc attatacggc ttttgcagcc aatagtcaaa cgcccttaag tatccaaatc
      841 gttgattgcc ttaatctcat ccgccgtcaa ctcccggtat gaaccggacg tcaatgactc
      901 atccagacac aacgggccca tagtcaaccg cttgaggtac aggactcgtt ttcccaatgt
      961 ctgaaacatc cgcttgatct ggtgaaactt tccttcatgg atctccacga taacctcgct
     1021 gacctccccg gagctgatca attccagccc cgccggaaga gtccggtagc cgtcgtccag
     1081 ttccacgccc gtagcgaaag cctgctgatc gactgttcct gccgcccccg tcacccgggc
     1141 gtaataccgt ttgggaacat ggttttgggg agccagtagt ctatgcccca gctccccgtg
     1201 attggtcaat aataacaacc cttcggtatc cttatcaagc ctgccgacag ggaacaatcc
     1261 ccgtttcggt atctccggtc caatgagatc gagaaccgtc cggccccggc catcttcagt
     1321 agcagtaaga acccctgccg gcttattgag catcaggtaa atgaactctc gataaactac
     1381 aatttccccg gcgcaagtca cctcatctga accgggatct accgccattg cttcattccg
     1441 gcaggtatca gagttgaccg tgacttggcc ggagcggatc aaccgcttga cttggctccg
     1501 actaccccga cccatattgg acagataccg atcgagacgc atcccgttca ccttcattcc
     1561 tcacaaattt ctaaggctga gaaaccgccg gtacgggagg ttgaaccgag tcggttcccg
     1621 tctgattccc cggagccggc gagggtgaaa ccaccggttc cgccctgttt gtagcgggag
     1681 tacccgggtt gggagtatcc gctccatccg tgccgggggc ttccgtctgc ggctcttctt
     1741 tttgccccgg gaaaagccaa tgccagagct tatatgccgc gctctgcgga acattttgtt
     1801 ccgcaaaaac ctctttataa ctgtttccag cgagccatcc cgggacagcc tgacctgtgt
     1861 cgggattgac atccgcccat acaatcccgg agggttgagt gaactgggtt accggccgac
     1921 ccacggtgat ggcacgcata taattgcccc agatctcagc ggccgacgca ctgccgatat
     1981 taccctcctt gtaccgcatg ggtaccccct gtcgatcatt tccgatccaa accgtggtca
     2041 acagatccgg agtatatccc acgaaccagg cgtttgtata atcactggtg gttccggtct
     2101 tacctgccgc cggtctctcc ggcagcttgg cccgaacccc ggtgccacga tcaatcacat
     2161 cctccatcag catggtcatg atgaaagcgg tctgcgggga caatgccggt tgcggtgaag
     2221 cgggagcaaa ttgcttcaac agattgccgt ggccgtccaa aaccttacga actgcgaacg
     2281 gtttgaccag attgccttga ttggcaaagg gagtataggc cagcgccaat tccatcgggg
     2341 tgactccctt ggtcaagccc cccagggcca gtggggccaa attcagatcg ttattcgaac
     2401 cgctcttaac cagactgttg atacccatct tttccatctg ttcggctact gcgtctaccc
     2461 cgactgcctg taaaagctgg accgccacgg aattaaccga ttgacggagt gcctcacgca
     2521 aggtcatccg gccgttataa gtgttatcat agtttgtggg tgtccaaacc gtgccattct
     2581 caagggtaat ggagagcggt ttatcctcaa agaccgatgc cgcgttatag gctcctttct
     2641 caaacgcagt ggcgtagaca aaaggtttca cagcggatcc cggctgacgg tatgcggtaa
     2701 tactccggtt cagttggctc cgggcataat cccgtccgcc aaccatcgcc aagattcctc
     2761 cgttggtagg gtctaatgtc accaaagccg cctgaggttg aatttttgcg gaaaaacccg
     2821 ggatttgctg caaggctaac tcggcccggc gttggtaatt caaatcgagc gtagtataga
     2881 tcttgtaacc gccccaacgc agttcgtctt cgctgaagcc gagattttta acaagatact
     2941 cccggacat
//
LOCUS       DASVMB010000313         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_332296,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000313 DASVMB010000000
VERSION     DASVMB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332296"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..862)
                     /locus_tag="VF531_08895"
     CDS             complement(263..862)
                     /locus_tag="VF531_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7714127.1"
                     /translation="MNSGIEDKRELVINTAIRFFTRQGFRKTSMDEIAHAARVSKATI
                     YQMFGDKSGLLGEVYKQKGKELYEKMLQAIAPGTATIPQLLQMLSFAREFCSQDPLFS
                     QFMDQREIEELEAIPQFQDIVSDSVDLIDKLLTQGIARGEIRPMPTRMASYFMFRIGF
                     YISQYGRELFTMFSMDDILNFFDSVLTRGIAADDGRRLD"
     gene            complement(1031..1948)
                     /gene="metA"
                     /locus_tag="VF531_08900"
     CDS             complement(1031..1948)
                     /gene="metA"
                     /locus_tag="VF531_08900"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEX7714128.1"
                     /translation="MPIKIPNNLPASETLNHENIFVMNEERAFHQDIRPLKIAILNLM
                     PLKITTETQILRLIGNSPLQIEIVLIHPKSYQSKNTSEEHLINFYKTFDEIQDQKFDG
                     LIITGAPVEQMEFEEVDYWEELTLIMDWAKHNVFSTFYICWAAQAGLYYHFGVPKYLL
                     PKKVFGVFPHRICRKYVKLMRGFDDLFYAPHSRHTEVRKQDIEKVPELEILAESPEAG
                     VYIVATKDGRHIFVTGHSEYDPLTLKWEYDRDISKGMAIDLPKNYFPDGDSTKEPIVR
                     WRSHANLLYVNWLNYYVYQETPYDLNEIG"
     gene            complement(2029..>2512)
                     /locus_tag="VF531_08905"
     CDS             complement(2029..>2512)
                     /locus_tag="VF531_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7714129.1"
                     /translation="VQNGFGAVLGPQDSWLLLRGIKTLRARMVIEQQAALLIAKWLQH
                     QSWVTDVYYPGLPEHPGYKIHQTQATGSGAVLSFKTDTPRRAIQIMQKVKLWSVAVSL
                     GGVESILSYPVKMSHASIPPKERERLGITDNLIRLSVGLEEAEDLMEDLETGSRSLGL
                     "
BASE COUNT          665 a          642 c          515 g          690 t
ORIGIN      
        1 cacggtggtc attaccgcat ccatgatggt cgtcttggag tcaagcaccc cgtccacctt
       61 acttaaaatc tcctctcccg tcagggcggc tgcgcccacg atgccggaga gggccaacaa
      121 tcccgtcaga caaacaacta accactgttt tctttttccc attgttctac actccttgat
      181 ctcttttttt tgttataaac acacttacca actcgcttgt aaaaaacgct ccttttcagc
      241 cgcgcacgct gaatccaagg tctcaatcca accggcgccc atcgtcggct gcaattccgc
      301 gggtcaatac cgaatcaaaa aagtttaaaa tatcatccat ggaaaacatc gtgaacaatt
      361 cccggccgta ttggcttatg taaaatccga tccggaacat gaaatacgag gccatccggg
      421 taggcatcgg ccggatctct cccctggcga tgccctgggt cagcaatttg tcgatcaaat
      481 ccaccgaatc actgacgata tcctggaact gcgggatcgc ttccaactcc tcaatctccc
      541 gctggtccat aaactggctg aagagtggat cctggctgca gaactcccgg gcgaaactga
      601 gcatctgcag taactgaggg atagtggccg tccccggggc aatcgcctgg agcatctttt
      661 catagagctc cttgcccttt tgcttataga cctctcccaa aagaccgctt ttatcgccga
      721 acatctgata aatagtggct ttgctcactc tggcggcgtg agcgatctca tccatgctgg
      781 ttttgcggaa accctgccgg gtgaagaagc gaattgccgt attgatgacc aattcacgct
      841 tatcctctat acctgaattc atttccgcct ccatgaacct gaaaaactaa tttagttttt
      901 tagttttatc tgatcattat attaccacac aacccaatat atgtcgagct gtgtaaatat
      961 taacgaatgg taaagacagt ggtttcaaat ttaaaaaaaa gactgtccat gaaagacagc
     1021 ttcacgatat ttatccaatc tcattcaaat catatggggt ttcctgatat acataatagt
     1081 tcaaccaatt tacgtatagc agattggcat gactacgcca tctgacgatg ggttctttgg
     1141 tggaatcccc atccgggaaa taattcttgg gcaggtctat cgccatcccc ttgcttatat
     1201 cacggtcata ttcccatttt agagtcaacg ggtcatattc cgaatgccct gttacaaaaa
     1261 tatggcggcc gtctttcgtg gcaacgatat aaactcccgc ctccggtgat tccgccagta
     1321 tttccaattc aggaactttt tcaatatcct gttttctcac ttccgtatgg cgtgaatggg
     1381 gtgcgtaaaa aagatcatca aacccccgca ttaatttgac gtacttcctg caaatcctgt
     1441 ggggaaaaac accgaatacc tttttgggca aaagatattt ggggacgccg aaatgataat
     1501 acaaaccggc ttgggcagcc caacaaatat aaaaagtgga aaacacatta tgtttggccc
     1561 aatccataat caaggtcagt tcctcccaat aatcgacttc ctcaaattcc atttgctcga
     1621 ccggagcgcc ggtaataatc aagccgtcaa acttttgatc ctgaatttca tcaaaagttt
     1681 tgtaaaagtt gatcagatgt tcttcagatg tgttcttcga ttgataactt ttaggatgga
     1741 tcaatacgat ctctatctgc aagggtgagt tgcctatcaa acgaaggatc tgtgtctctg
     1801 tggtaatttt aagcggcatt agattcaaaa tggcaatttt aaggggccgg atatcttgat
     1861 ggaaagcccg ttcttcattc atcacaaaga tattctcatg attcagcgtt tcactggcag
     1921 gcaagttatt gggaatctta atcggcataa tatttaaccc cctgaatctt tattgtttcc
     1981 aaatataaag ttaggctaat gtttgtcgat cctttgggta ctcatccatt acaagcccaa
     2041 tgatctggat cccgtttcca agtcctccat cagatcttca gcctcctcca gtccaaccga
     2101 caaacggata agattgtcgg tgattcccaa tcgttcccgt tccttcgggg ggatcgaggc
     2161 atgagacatc ttgaccggat aggacaggat ggattcaacg ccccctaggc taaccgccac
     2221 ggaccacaat ttaacctttt gcatgatttg aatggcgcgt cgaggggtat cggtcttaaa
     2281 tgataaaacc gcgccggacc cggtggcctg tgtttgatga attttataac ccgggtgttc
     2341 gggtaagccc ggataataaa catccgtcac ccacgactgg tgttgcagcc atttggcgat
     2401 taataacgcc gcttgctgtt cgattaccat ccgggctcgc aacgtcttaa tcccccgtaa
     2461 caacagccaa ctgtcctgcg gccccaaaac agccccaaag ccgttttgaa ca
//
LOCUS       DASVMB010000314         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_114025,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000314 DASVMB010000000
VERSION     DASVMB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114025"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..2188)
                     /locus_tag="VF531_08910"
     CDS             complement(407..2188)
                     /locus_tag="VF531_08910"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX7714130.1"
                     /translation="MNKQYSVVFKNRDIVRQVTEGTLLESIIREVEPGFVFPCNGRGS
                     CGKCRVRAEGELTPPTSTEIRHLGSAALAAGVRLACQAKVIGPVQVKLPEMISGEAIL
                     SAGYLRSQAPDPLWSRVRLEKPEPLTWEAIASVLPADCRPSLNVLREIPRLPRETPEL
                     SLDILNGQVIGVTAGSDPGACYAVAVDIGTTTLAAYLVNLATAGIERTVSAFNPQGSY
                     GADLISRISHAATTEGLEELHRAVIAAVNRFVVELACAAGIHLSQIIQVNLAGNSCMS
                     QLFLKVDPESLGRLPFEPVVKDLVRMTPAELGLTINADGLVYVLPGIGGFIGSDISAG
                     ALACELNGDKTELFIDIGTNGEIILTGKGRMLASSTAAGPAFEGAKISCGRLAGPGAV
                     ADLQFTPAGLELVTIGNQPPSGICGTGLIRAVVELVKRGIIEETGRFADGLSDPRYDP
                     EQKRYYLTERNVSDQEVEPVYISQQDIRQFQLAKGAVYAGIHLLMKKLGLKPEDLSTV
                     YLAGAFGSYLKPEDAVFLGLLPPVPLDRIKAVGNTAGMGTVITVLSQTALEDLRLLTR
                     RIEHVELANDPEFTEIFTEAMMFRDSD"
     gene            complement(2307..>2676)
                     /locus_tag="VF531_08915"
     CDS             complement(2307..>2676)
                     /locus_tag="VF531_08915"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010271043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7714131.1"
                     /translation="VGNLDPRRDFSDVRDVVRGYWLALKKGKAGEVYNICSGNALTIR
                     QILDILLSFAKCSIEVKVDPSRMRPSDVSVLLGSYKKFSKISGWEPAIPFERTLADLL
                     EYWRQRLETTAGGNGFYSRP"
BASE COUNT          682 a          738 c          640 g          616 t
ORIGIN      
        1 tgcagcaatt cagattcaaa attttgttcc gttagattca ccattgaaat tcctccttat
       61 caatataccc tacagggtat agtatatcag attctacaaa aaagtcaacc atgccattat
      121 ctcaatgatc cgataagaaa ctcctgtaga tttgagcgaa aagtgggatc cgaaagatgt
      181 ttaaaacatt gttgatctat gataaactgt aactaataca tcaataatgc cataatttag
      241 aaattgcaaa aaggaagtga agatgatggg ccgcagaccg gtctgaaaga accgcacgcc
      301 cttccatctt gatttacccc aggaacatcc gcgcgtcttt tccttcatga ctacctccag
      361 gaacataagc acgcacttac atcctgattt ggcattcaac caataactaa tcactatccc
      421 taaacatcat tgcctcagta aaaatctcgg taaactcagg atcgttagct aactccacat
      481 gttcgatcct gcgggtcaat aagcggagat cctccagggc ggtttgggag agtaccgtga
      541 tgaccgtgcc cattccggct gtatttccta cggctttgat ccggtccagt ggtacagggg
      601 gcaataatcc caaaaagacg gcatcctcgg gcttaagata ggatccaaag gcgccggcca
      661 gatacacggt agataaatcc tccggtttca aacccagttt cttcatcaag aggtgaatcc
      721 ccgcgtaaac agcccccttg gctagttgaa actggcgaat atcctgctgg gagatgtaga
      781 ccggctccac ttcttgatct gatacattcc gttcagtgag ataatagcgc ttctgttccg
      841 ggtcgtaacg cggatcactt aatccatccg caaaacgccc ggtttcctca atgattcccc
      901 gcttcaccag ttcaactacg gcgcggatca atccggttcc acagatgccg gagggcggct
      961 ggttgccgat ggtcactagt tccagtccgg cgggggtaaa ttgcaaatcg gcgacggctc
     1021 cgggtccggc caacctgccg caggagatct tggccccctc aaaagcgggc cctgctgcgg
     1081 tggaactggc cagcatccgg cctttgccgg tcaggataat ctcgccgttc gtgccgatat
     1141 cgataaacag ctcggtttta tccccgttta attcgcaggc aagcgccccg gcgctgatat
     1201 cggaaccgat gaaaccgccg atccccggta gcacataaac caacccatcg gcattgatgg
     1261 tcaggcctaa ttcagccgga gtcatccgta ccaggtcctt gaccaccggt tcaaaaggta
     1321 acctgcccag ggattcgggg tcgactttca aaaacagttg gctcatacag gaattgccgg
     1381 ccagatttac ctggataatt tgagatagat gaatcccggc ggcacaagcc aattccacga
     1441 cgaaacgatt aacggctgca atcactgccc gatgcaattc ttctaaaccc tccgttgtcg
     1501 cggcgtgact gatccgtgag atgagatccg cgccgtaact gccctgagga ttaaaggccg
     1561 aaacggttcg ctcgatcccg gcggtcgcca gattcaccag ataggcggca agggtggttg
     1621 tgccgatatc aaccgctaca gcatagcagg ctccggggtc ggagccggcg gtaactccga
     1681 tgacttgtcc attcaggata tcgaggctca attccggggt ttcccggggt aaccgcggaa
     1741 tctcccgcaa tacgttcaat gacggacggc aatccgctgg tagaacggaa gcaatggctt
     1801 cccaggttaa cggttccggt ttttcaagtc taaccctgct ccacaaggga tccggagcct
     1861 ggcttcgtaa ataaccggcg ctcagaatgg cttcgccgga aatcatctcc ggtaatttta
     1921 cttgaaccgg tccaattacc ttcgcctgac aggcaagacg aaccccggcg gcgagtgccg
     1981 ccgatcccag gtggcgaatc tcggtggagg tcggcggggt aagttcaccc tcggcgcgaa
     2041 cccggcattt gccgcagctt ccccgcccat tgcagggaaa aacaaaaccg ggctccacct
     2101 cccggataat gctttctaaa agagtcccct cagttacctg ccggacgatg tcacgatttt
     2161 taaagactac cgaatactgt ttattcatcc cttgactcca atattagttg ataagatgtc
     2221 cagatgataa aggcatttaa atccattatt attcattata cctaaatcta cccggactgt
     2281 ccacggagag acccggcgca aggtttttaa ggtcgggaat aaaaaccgtt tccaccggcg
     2341 gttgtctcga gccgctgccg ccaatattcc aataagtcgg ccaaggtccg ttcaaaggga
     2401 attgccggtt cccagccgct gatcttggaa aacttcttat aggaaccaag cagcactgat
     2461 acatccgagg gacgcatccg ggaggggtct accttaacct caatggaaca cttggcgaaa
     2521 ctcagcagaa tatcgaggat ttgccggatg gttaatgcgt tcccgctgca aatattgtaa
     2581 acctccccgg cctttccttt ctttaaagca agccagtatc cgcgcacgac atcccggaca
     2641 tcgctgaaat ccctccgggg atcaagattg ccgaca
//
LOCUS       DASVMB010000315        14839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_553938,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000315 DASVMB010000000
VERSION     DASVMB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553938"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF531_08920"
     CDS             complement(<1..379)
                     /locus_tag="VF531_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7714132.1"
                     /translation="MEDWRQQVETDLKGIIQSRDVPMSRITTFRIGGPLDLLTEPMDL
                     GELRRVLAFCQKYHLPWFVLGLGSNLLVRDKGIRGVGIRLGGDFKSWRAEGVQIRAGA
                     AVTLADLSRGTACLGLTGLEFACG"
     gene            418..1461
                     /gene="splB"
                     /locus_tag="VF531_08925"
     CDS             418..1461
                     /gene="splB"
                     /locus_tag="VF531_08925"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HEX7714133.1"
                     /translation="MIRYHITMKLFEPKRAFFEEASLEYPLGKKILKQLDAAGIPVRV
                     IGSHNRVTGIPGVNAMQSYREGKRTLVVGVKRDLHLSTCKPSADFEFALATSCPGGCQ
                     YCYLQTHLGKKPYIRVYVNIDEILSRVKTLSDKDPEKIITFEAASTSDPLAVEHITGS
                     LARTIDFIGELSNARLRVVTKFSNVTPLLDLEHHGHTRFRFSINTPTVVKRFEQNTAP
                     LEERIAAARRMSQAGYPVGFIIAPLFIYPDYQQDYASLLDQVAAALQPIPPDLTFELI
                     SHRFTRSAKKVIEERFPGTLLDLDESRRRPKYGKYGLVKFIYPEVEYHQLQQHLYSLI
                     AHFFPTARVEYFT"
     gene            complement(1535..1810)
                     /locus_tag="VF531_08930"
     CDS             complement(1535..1810)
                     /locus_tag="VF531_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEX7714134.1"
                     /translation="MKEVGILDRIREDLNLFVGKPIRIKANRGRKRIFEVEGILEQTY
                     PKVFVVRFKERQVERRISYSYADLLTKAVELSDGDNRIGLSAESATG"
     gene            complement(2052..2522)
                     /locus_tag="VF531_08935"
     CDS             complement(2052..2522)
                     /locus_tag="VF531_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HEX7714135.1"
                     /translation="MFFISPSRGRLTFDQVFKDMTDFIKQNPDSKFKLIVGTDSQLRE
                     EVCYVTAIIILREGKGGRFYYAKERERTKLGLKQRIFYETSRSLSVAAKLAKKLAENG
                     WSDPEIEIHLDVGEQGKTKDIIREVVGMVTGSGFDARIKPDSYGASKVADKFTK"
     gene            complement(2569..3438)
                     /gene="rsmA"
                     /locus_tag="VF531_08940"
     CDS             complement(2569..3438)
                     /gene="rsmA"
                     /locus_tag="VF531_08940"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7714136.1"
                     /translation="MMEKPTSLRVIKNALAREGLAPRKSLGQNFLCDANIPNKIVAAG
                     AVEKTDLVLEIGPGLGVLTSILIEQAGMVIAVEYDRGLFRILQENLATVPNLRLINQD
                     FLQVDLWSLLEPLRNDFPVFKVIANLPYYITTPVLFKLVESGLHWGGMVFLIQKEVAE
                     RLCAGPGSDAYGAVTVMINFFGTVEKVAMVPRTVFYPVPQVDSAAIRITPHPGPTELY
                     PSLHRVVQAAFGQRRKTILNALFAGGILLGSNEKSELAVSLRDLGIDPERRGETLALE
                     EFIKIARIAAMAP"
     gene            complement(3450..4385)
                     /locus_tag="VF531_08945"
     CDS             complement(3450..4385)
                     /locus_tag="VF531_08945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7714137.1"
                     /translation="MDEERILIVEPESRIAELAVLKLSNAGYLVSNAVNIEDALKKLN
                     ANPPDIIIANPNLPASSGDGYEFCNEVRHNPKWMKIPLVLLVDQEFNEEQFKQWGVKV
                     DDFLIKPFSPKALLNKVNSLMVKIRLTKALNPLTELPGKVHLVEALNSRLADGNKLAL
                     IFADLKKFAMYNQNYGFDKGDEVIRFLAGVLHDQIEQSGTNADLYHLGGDDFCILMDN
                     GDPAGLCEAIIHRFDEEIAGFYPEGDRNRGGMVMANRQGIIEQWPILTIGLGIVSNER
                     RTLSGWLQAEAIGAELLKYCKTMPGSQYSRDRRGA"
     gene            complement(4504..5043)
                     /locus_tag="VF531_08950"
     CDS             complement(4504..5043)
                     /locus_tag="VF531_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7714138.1"
                     /translation="MNNSELFLLSLINQAPRYGYEIAQFLEESNAGLWLNLSMPYVYR
                     LLKGFEAREWVRSRSVESDHNRPNKNIYEITELGQAVLVKTLTTIDFNDDKIYFGTDV
                     ALAVYTITGQSFDLPTLIRNRTRHVTEELNRFAASDTQGDLSADEIMARLIIEHRISF
                     LRAELEWLQKVQGALDKMA"
     gene            complement(5151..5831)
                     /locus_tag="VF531_08955"
     CDS             complement(5151..5831)
                     /locus_tag="VF531_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7714139.1"
                     /translation="MDPLNSDLAKEFSGLDQLFRIYHQLDLDIAGLKIRTGLDCIPSC
                     RSCCATSQTNIEVSLFELIPLAIHLWEVNEAEFWLDRTAAAGTNSPCVLYMADPKESP
                     ISGCRYYQLRPLICRLFGYGAIRDKYGKFRAVICRSMKLLRPDLVHRAEQLMDTGLEV
                     PDFSSVTRSVAVLNPYLSEPRYPINQAFSLALEKVGYRRSLLRSAGECNEENPDPTKP
                     HPHLGRTA"
     gene            complement(5957..7360)
                     /locus_tag="VF531_08960"
     CDS             complement(5957..7360)
                     /locus_tag="VF531_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714140.1"
                     /translation="MRLKGHNRFIYLGLILLFLVGLMIPIYLRFSRVKLGDREIHCNP
                     RARINPRKEYHIRLWDYDRPILTSRDGYRRYLEEAIAAFRKQYPGVHVEVRLLEASTG
                     PKELRQALENHTAPDLYCSAWEIPSFDWDCQIPVGFFLKDTEQELYYPGLLNLVRLYG
                     VVCYFPYWTAPRFWIGNRRLLEKAGFNIGLLQQSGWSMSELLVAVKRAGPGRYLLVGD
                     SGSNGFLTQLSVNSMKGLEQYRPKVTDIREALGFLDTLRDQKALPEDFDTRMLEYFLN
                     GKAMVLAGANLSLYGWVKNYLRDRQSNVTPSDGSEPNSSKDNRDGLTQWDPVLLPVPH
                     QTDAAARLLAENGVIAVYRSKFTIGDDQIAVAVKLGQFLSTYRQTRPWQELLVCPAAM
                     ANTEYWAAGTGLTPSDLRLIDQSLRHTSLLNLNFSPIRAQEFDRVIREYYLRKINLEI
                     AESRLAQLMNEPGAISF"
     gene            complement(7396..8238)
                     /locus_tag="VF531_08965"
     CDS             complement(7396..8238)
                     /locus_tag="VF531_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7714141.1"
                     /translation="MTGKSFSKLLIILWAVILIVMTVSLQFRSGIFQVRQSALREMLL
                     KKDRKDAFFIGLRRIIGNNTVQTKVTCEDGDEFTLMERDITTANNPVGNGDLINIDTK
                     QLHLTLYRDGKVFRRYPVAVGEPDTPTPIGEWRIIHKGGNWGNGFGVRWMGLNVPWGV
                     YGIHGTNKPWSIGTRASHGCIRMFNPHVTELYDLVKLGTPVHITGNLRPAVPRADIGR
                     NNTGRDVVYMQFALRKAGFDPGTVDGRFGEKMEQAVQQLQLFYGLTPTGRIRSNEQYL
                     LKLK"
     gene            complement(8444..9106)
                     /gene="phoU"
                     /locus_tag="VF531_08970"
     CDS             complement(8444..9106)
                     /gene="phoU"
                     /locus_tag="VF531_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7714142.1"
                     /translation="MTDISEQVGDQTAQHLLKMGNLAAESLKLAIQALRDQDVKLALK
                     VIEQDDQLDNLQIVVEEEIERGIGTNRNPSLTTFDLRRDLAMAKIGVDLERIGDYATN
                     IAEVVMELKDESYLKPLVHIPQMANLVMNMVNTSLKAFIEKNADLADAVCQRDDEADN
                     LYEEVREELIRITGQESDPRKAYQASRFLLIAEWLERAGDHATNIGEATIHIVTGKRV
                     KY"
     gene            complement(9191..10099)
                     /locus_tag="VF531_08975"
     CDS             complement(9191..10099)
                     /locus_tag="VF531_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7714143.1"
                     /translation="MTKSDQLLAVIAGIVVSLIFGLSFLFTKNSLSLMSPIELLANRF
                     TLAAIFLFLLNLTGVVRIRLNLKRFAGILPLTLFQPILYFLGETFGIQLTSASESSLV
                     IALIPVAVTILGGLFLHEQAGWRQWLFVLCSVAGVGMIVLAGSGANLAVHTAGMAILL
                     LAVAAAAVYNILSRRYSATYSPLEITTGMMYAGAVFFNLLRLTAFPAKGFYFQGFTRI
                     GSLFPLIYLGLLSSVGAFFLVNYMLKKMAVAKVAAFNNLTTVVSVAAGVIFRGEALGP
                     YHLVGGSLILIGVWGINALDKSKAMG"
     gene            complement(10173..10622)
                     /gene="rimI"
                     /locus_tag="VF531_08980"
     CDS             complement(10173..10622)
                     /gene="rimI"
                     /locus_tag="VF531_08980"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7714144.1"
                     /translation="MQPEISPMRVEDLEEVLKIEDLSFPTPWSRNSFLYELLENERAI
                     YLVAKSPLGNVIGYVGIWVIFDEGHITNLAVHPGYRRQGVGKALMLALVEAVRKRDVR
                     HLTLEVRRTNLAAQDLYQKLGFVHMGVRRKYYLDNNEDALIMWKGPI"
     gene            complement(10564..11313)
                     /gene="tsaB"
                     /locus_tag="VF531_08985"
     CDS             complement(10564..11313)
                     /gene="tsaB"
                     /locus_tag="VF531_08985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX7714145.1"
                     /translation="MLILGLDTATPWGTLALWEDGEVVAEAGLKTGKGGGEYLLDLLG
                     TMIQKSGREFRKIDLIAVGTGPGSFTGIRVGLAAVKGIAAGIERPVFGLSTLRVIAEN
                     ARYIPVTYIATVLDARRERVYAALYRKSDGEILECLKMPQVMAATEFARQLAAYSGTV
                     ICGDGCKKYRSIWELVPGLMIGPEEWARPSAGKLAAIAFRDWDRRTSVLSELTPFYLG
                     KVEAEARLEEGLNATGDQPDASRRFGRSIED"
     gene            complement(11294..11770)
                     /gene="tsaE"
                     /locus_tag="VF531_08990"
     CDS             complement(11294..11770)
                     /gene="tsaE"
                     /locus_tag="VF531_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7714146.1"
                     /translation="MKSISLTLKTAELTYEMGRLLGELVKPGDLLFLFGELGAGKTTL
                     TKGIAYGLGVTAAVTSPTFQLLKSYQGRYSLYHLDLYRLKSSLELDILEPEVLAETGV
                     MVIEWGQLLLERLGPTYLEIIMEFAPDGAGRIVTINSKGPEYDHFLESLSHAHLGS"
     gene            complement(12022..12381)
                     /locus_tag="VF531_08995"
     CDS             complement(12022..12381)
                     /locus_tag="VF531_08995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX7714147.1"
                     /translation="MVSTIRRGDIFYANLNPVIGSEQGGLRPVLVVQNDIGNTYSPTT
                     IIAAITSKIKRAKLPTHIEISAARYQLEKDSVILLEQLRTIDKQRLRERITRLDEEIM
                     ARVDEAIAISLGLKALS"
     gene            complement(12386..12697)
                     /locus_tag="VF531_09000"
     CDS             complement(12386..12697)
                     /locus_tag="VF531_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX7714148.1"
                     /translation="MAQAKRIMVSLPDSLLQEVDGIVRRETGNRSAFVREAMLLLIAE
                     RRHHEQMERYRLGYEKTGKLNLALAEDGLDEDLRDLEQYENYLRAGAHSVNAGTRTRR
                     D"
     gene            complement(12916..13563)
                     /locus_tag="VF531_09005"
     CDS             complement(12916..13563)
                     /locus_tag="VF531_09005"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEX7714149.1"
                     /translation="MKQAILVGGGPVSLKQLKQELTIRPEILYAADRGATYLLELGVM
                     PDLLVGDMDSFAESDLSRLAAAGVQIEKYPEAKNETDLELVVGQALKAGALRIRIFGG
                     LGGRFDHTLGNLGLLVNSLEHGVEAYLIDETHEITATSRDIVLTAKPGWAVSLIPWTN
                     RVTGVTTTGLKFPLQNDTLYFSHTRGIHNQFTAATARVTMKTGILLIVSFKEDQF"
     gene            complement(13892..14302)
                     /gene="rpsI"
                     /locus_tag="VF531_09010"
     CDS             complement(13892..14302)
                     /gene="rpsI"
                     /locus_tag="VF531_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7714150.1"
                     /translation="MPRAKKTTKSMYYGTGRRKCAIAKVRLVPGQGNVIVNGKPVMDY
                     FGRKVLELTVRQPMATTATEGKYDVYASVHGGGISGQAGAVRHGIARALLRVEDEFRL
                     PLKKAGFLTRDPRMKERRKYGLKKARKAPQFSKR"
     gene            complement(14320..14754)
                     /gene="rplM"
                     /locus_tag="VF531_09015"
     CDS             complement(14320..14754)
                     /gene="rplM"
                     /locus_tag="VF531_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7714151.1"
                     /translation="MQTYMAKPEEVQHKWYLVDAEGQTLGRLASTVAMILRGKNKPTF
                     TPNVDCGDHVIIINAEKVVLTGKKAEDKIHYTHSMYPGGLKAIKYGQLLKNKPEYALT
                     RTIWGMLPHNSMGRKMIKKLRVYRGNEHPHQAQVPEKLEIKA"
BASE COUNT         3862 a         3985 c         3203 g         3789 t
ORIGIN      
        1 ttccacaggc aaactccagt cccgtaaggc ccaggcaagc agtgccccgg gataaatccg
       61 ccaaagtaac cgcggcaccg gccctgatct ggacgccttc agcccgccag gacttaaagt
      121 ccccgcccaa tcgaataccc acaccccgaa tacctttatc ccgcaccagc aaattcgaac
      181 ctagccccaa tacaaaccac ggcaaatgat acttttgaca aaacgcaagc acccgccgga
      241 gctccccgag atccatcggc tccgtcaaaa gatccagggg tccgccaatc cgaaatgtag
      301 ttattcggga catcggcaca tccctgctct ggataattcc cttcaaatcc gtttccactt
      361 gttggcgcca gtcttccatt aaaaaccccc ctttaagggc ccaggaatat attatggatg
      421 attcggtatc atattaccat gaagctattt gaaccgaaaa gggctttctt tgaggaagcc
      481 tcccttgaat atccactggg aaaaaagatc ctgaagcaac tggatgcagc cggcattccg
      541 gtccgggtta tcggttccca caaccgggta accggaattc cgggagtcaa tgccatgcaa
      601 agttaccgcg aaggtaaaag gactctggtg gtaggagtaa aacgggattt acacctctcc
      661 acctgcaaac cctcggccga cttcgagttc gcattggcga caagctgccc cggaggttgc
      721 caatactgtt atctccagac ccatctgggt aaaaagccct acatcagggt ttatgtaaat
      781 atcgacgaaa tcctgagccg ggtaaaaaca cttagcgata aagatccgga aaaaatcatc
      841 acctttgaag cggccagcac ttcggatccg ttagctgttg agcatatcac cggctcactc
      901 gcccgaacca tcgatttcat cggagaattg agcaatgcca gactacgcgt ggtgaccaag
      961 ttttctaatg tcactccgtt gcttgacttg gagcatcacg gtcatacaag attccggttt
     1021 agtattaaca ccccgacggt agtgaaacgt ttcgaacaaa acaccgcccc tctggaagaa
     1081 cggatcgcgg cagcccgccg gatgagccaa gccggttatc cggtaggttt catcattgcc
     1141 ccgcttttca tctatccgga ttaccagcaa gattacgctt cgctgcttga tcaggtcgca
     1201 gccgccttac aaccgattcc accggactta acctttgaac tgattagcca tcgctttacc
     1261 cgtagcgcca aaaaggtaat cgaggaacgg tttcccggga cactgctgga tctggacgag
     1321 agccgacgtc gtccgaaata cggtaaatac gggcttgtta agtttatcta cccggaagtt
     1381 gaatatcatc aattacaaca gcatctatac tcattgatcg cccatttttt ccctacagcg
     1441 cgggttgaat attttaccta aaaaataaaa ccgctggaaa tctagatcat gatgacaagc
     1501 aggaaaaaag cccgtcattt gacgggcttt aagcttagcc ggtggctgat tcggcggaca
     1561 acccgatccg gttgtcgccg tctgaaagtt ccacggcttt tgttaaaaga tcagcataac
     1621 tataagagat acgccgttcg acttgacgct ctttaaaacg gactacgaag actttgggat
     1681 aggtttgttc gaggattcct tcaacttcga aaatacgttt ccttccccga ttggccttta
     1741 ttctaatcgg tttccctacg aatagattta aatcttcgcg gatacggtct aaaatcccaa
     1801 cctccttcat ccaattcacc atccttttct gctaaacaaa agtataccat ggctcgtccg
     1861 catttgtcaa gcaaaatatt atactggaac tatcgatact tgtcaacccc attttaagtg
     1921 agtttttcaa ttttgttaaa agaaaaatag cggttggtac tggccgatcc gctatctatc
     1981 cttctaaatc tcggcttcac gccatggatt gaaaaaatca aggcgcacaa acaaccgcta
     2041 cagatataaa attacttggt aaacttatcg gccacctttg atgcgccata ggaatcgggt
     2101 ttaattcggg cgtcaaaacc actgcccgtg accattccta ccacttcacg aatgatatcc
     2161 ttggttttac cctgttcacc cacatccaaa tgaatttcga tctccgggtc cgaccagccg
     2221 ttttccgcaa gtttcttggc taatttggcc gccaccgaca ggcttcggga agtctcgtaa
     2281 aaaatccttt gtttcaaacc aagtttggtc cgttcacgtt ccttggcata gtaaaaacgg
     2341 ccgcctttcc cttcccggag aataatgatg gccgttacat aacatacctc ttcacgaagt
     2401 tgggaatcag tccctacgat taatttaaac ttagaatccg ggttctgttt aatgaaatcg
     2461 gtcatatctt taaaaacctg atcaaaggtt agccttcccc gggatggact aataaaaaac
     2521 attgttgcac cttctaagtt acaagtctaa aacccggaga accgtcatct atggcgccat
     2581 tgcggcaatc cgggcaattt ttataaattc ctcaagagcc agcgtctcgc cacgacgttc
     2641 cgggtcgatc ccaagatcgc ggagactgac tgccaactca gatttttcat tactccccaa
     2701 caatattccg cctgcgaata atgcatttaa aatcgtctta cggcgttgac cgaaagcggc
     2761 ttgaaccacc cggtgaagac taggatagag ttcagtcggg ccgggatgtg gtgtaatccg
     2821 gattgccgcc gaatccacct gagggacagg gtaaaacact gttcgcggta ccatcgccac
     2881 tttctccacg gtaccgaaga aatttatcat cacagtcaca gcgccgtatg catcgctgcc
     2941 cggcccggcg cataaccttt cggcgacttc tttttggatt aagaaaacca taccccccca
     3001 gtgcagacca gactcaacca gcttgaaaag cactggggta gtaatatagt agggaagatt
     3061 ggcgatgacc ttaaagacag gaaaatcgtt acgcaatggt tcaagtaatg accaaagatc
     3121 gacctgcagg aagtcttgat tgatgaggcg taaatttgga accgtggcca aattctcttg
     3181 caagatccgg aaaagtcccc gatcatattc aactgcaatg accatcccgg cctgttcgat
     3241 caggatggag gttaagacgc ccaaccccgg tccgatctcc aaaaccaaat ctgttttttc
     3301 aaccgccccg gcagcaacaa tcttgttagg aatgttggcg tcgcataaaa agttctgtcc
     3361 caggcttttc cggggggcca aaccctcccg agccagagca tttttgatta ctcttagaga
     3421 tgtcggtttt tccatcatcc gtttatatct tacgcgcctc gccggtccct cgaatactgg
     3481 ctccccggca ttgttttaca atacttcagt aattcggcgc caatcgcttc ggcctgcaac
     3541 caacccgaga gtgtacgccg ttcgttactg acgataccga gtccaatggt tagaatcggc
     3601 cattgttcga tgattccctg gcggttggcc atgaccattc caccgcgatt gcgatcaccc
     3661 tccgggtaaa aaccggcgat ctcctcatcg aaacgatgga taatcgcttc acaaagcccg
     3721 gccggatcac cgttgtccat tagaatgcag aaatcgtccc cgcccagatg gtacaggtct
     3781 gcattggttc cactctgttc aatctgatca tgaagcactc ctgctagaaa ccgaatgact
     3841 tcatcgcctt tgtcaaatcc gtaattctgg ttgtacatcg caaatttctt tagatccgca
     3901 aagataagcg ccaatttatt tccatcagcc aagcgtgaat tcaaggcttc caccaaatga
     3961 accttcccgg gaagctcggt caggggatta agcgccttgg tcagacgaat ctttaccatc
     4021 agggagttga ctttattcag taacgctttg ggactaaacg gtttgatgag gaaatcatct
     4081 actttaacgc cccattgctt aaattgttcc tcgttaaact cttgatcaac caataaaacc
     4141 agaggtattt tcatccactt cggattatgc cggacttcat tgcaaaactc atacccatca
     4201 cccgatgatg ccggtaaatt gggattggcg atgatgatat ccggcgggtt agcattcagt
     4261 tttttaagag catcttcgat attcaccgcg ttgctgacca aatagcccgc gttggaaagc
     4321 tttaagaccg ccaattcggc tattctgctc tccggctcca caatcagaat ccgctcttca
     4381 tccatcgcga tccctccaat aaaatactta ccatgaagat tcgacaaccg cccacccatt
     4441 cctgccgacc gggtctgaaa agccttttgg acccaaaagg ataccgtaac ttgattccct
     4501 gattcatgcc attttgtcca gagcaccctg aactttttgc agccattcaa gctcggcccg
     4561 gaggaaactg attcgatgtt caataatcaa tcgggccatg atctcatccg cagataaatc
     4621 gccttgcgta tcactggctg caaaccggtt tagctcttcg gtgacatgcc gggtccggtt
     4681 tcgaatcaac gtaggcagat caaagctctg tccggtgata gtgtaaaccg cgagtgccac
     4741 atctgtcccg aaataaatct tatcatcgtt gaagtcaatg gtcgttaatg tcttgacgag
     4801 aaccgcctgt cccaattcgg taatctcata aatgttttta ttgggccggt tgtgatccga
     4861 ctccaccgaa cgtgatcgaa cccattccct ggcctcaaac cccttaagta accgatagac
     4921 atagggcata ctcaaattca gccagagacc ggcattactc tcctctaaaa actgggcgat
     4981 ctcatacccg taacgtggag cttggttaat caaactcaac agaaacaatt ccgaattatt
     5041 cattggtcgc accccaaata ctaatgataa gaatattata catgtaaaat ataatacaag
     5101 taaatattca ttaggataat atttacttgt agaatttatt cttaccttac ctaagcagtg
     5161 cggcccaaat gaggatgggg ttttgttgga tctgggttct cttcattaca ttccccggca
     5221 gaccttaata gactgcggcg atatcccacc ttctccaaag caaggctaaa cgcctgatta
     5281 atcggatacc tgggttcgct caaatagggg ttaagcactg caaccgaccg ggtaacactt
     5341 gagaaatccg ggacttctaa ccctgtatcc atgagctgct cggcacgatg gaccaaatcc
     5401 gggcggagga gtttcatgga tctgcatatt accgcccgga actttccata tttatcacgg
     5461 atcgccccat agccgaatag tcgacaaatg agcggacgta gctggtaata gcggcagcca
     5521 ctgatgggac tctcttttgg atccgccatg taaagcacgc agggggaatt tgtcccggca
     5581 gccgctgttc ggtcaagcca aaactcggcc tcattcactt cccagagatg gatggcgagg
     5641 ggaatcaact cgaatagact aacctcaata ttcgtttggg acgtcgcgca acaacttcgg
     5701 caggaaggaa tgcaatcgag gcccgttctt attttcagtc cggcgatatc caaatctaat
     5761 tgatggtaaa tgcgaaacaa ttgatcaaga cccgaaaatt ccttggccaa atccgaatta
     5821 aggggatcca tatccaccat cacctcaata aaatgtaagg gcaacccgga ggttgtccaa
     5881 atgattgtct ttcaaccgat tctgatccta attatacacg tttcagttca tttgggaata
     5941 cccgataaca gaagagtcaa aaagagattg cgccgggttc attcatcaat tgtgccaacc
     6001 ttgattctgc tatctccaga ttgatttttc gcagataata ctcccggatc acacgatcaa
     6061 actcttgggc cctgatgggt gaaaaattca gattaagaag cgacgtatgc cttaaggact
     6121 gatcaatcaa tcgcaagtca ctcggggtca aaccagttcc tgccgcccaa tattccgtat
     6181 tcgccatcgc cgcaggacag accagtaatt cttgccaagg ccttgtctgc cggtaagtgc
     6241 ttaaaaattg acccagtttc acggctacgg caatctgatc gtccccgatg gtaaatttgc
     6301 tgcgatagac tgcaatgact ccattctcag ccaacaatcg cgccgcagca tcggtttggt
     6361 gaggtacggg taataatacc ggatcccatt gagttaatcc atcccggtta tctttagatg
     6421 aattcggctc acttccgtcg gatggagtta cattcgattg acgatctcta agataatttt
     6481 tgacccagcc atacaggctg agattggcac cagccagaac catagccttt ccgtttagga
     6541 aatattccag catccgggta tcaaagtctt ccggtaaagc cttttggtcg cgaagtgtat
     6601 ccaaaaaacc aagtgcttcc cggatatcgg taactttggg acgatattgt tcaaggcctt
     6661 tcatgctgtt caccgataat tgggtgagaa agccgttact tcctgaatca cctaccaata
     6721 aatacctgcc aggcccggct cttttaacgg caaccaacaa ttcggacata ctccaaccgg
     6781 attgttgtaa taatccgata ttgaaaccgg ctttctccag aagcctccgg ttaccaatcc
     6841 aaaaacgcgg cgccgtccaa taagggaaat aacataccac cccgtataat cggacgaggt
     6901 tcaagagtcc cggataatac aactcttgtt ccgtgtcttt taaaaagaac cctaccggga
     6961 tctggcagtc ccagtcgaaa gacggtatct cccaggcgga acagtaaaga tccggggcag
     7021 tatgattctc aagcgcttgt cttaactcct tggggccggt tgaggcctcc aataaccgga
     7081 cttcgacatg gactccgggg tactgttttc tgaaagcggc tatggcctct tcgaggtaac
     7141 gccgatatcc gtcccggctc gtcaggattg gccggtcata atcccataac cgtatatgat
     7201 actctttacg cggattgatc ctggctctgg gattgcagtg gatctcccga tcgcccaatt
     7261 tcactctgga aaatcgcaaa taaatcggga tcatcaaccc taccagaaat aaaaggatta
     7321 agccaaggta gatgaagcgg ttatgacctt ttagtcgcat tggctccttc accttctcct
     7381 tcccgcaccc cctggtcatt ttagtttgag cagatactgt tcgttagaac ggattcttcc
     7441 tgtgggtgtt aggccataaa aaagctgcag ttgctgtaca gcctgttcca ttttctcgcc
     7501 gaaacggccg tcgactgttc ccgggtcgaa cccggccttg cgcaaagcaa actgcatata
     7561 aactacatca cggccggtat tattgcgtcc aatatccgcc cggggcacag ccggtcgtaa
     7621 attaccggtg atatgaaccg gagtccccag tttcaccaaa tcataaagct cggtcacatg
     7681 ggggttgaac atccggatac agccatggct ggcacgtgtt ccgattgacc agggtttatt
     7741 ggtaccgtgg ataccgtaga ctccccaggg tacattcagt cccatccaac ggaccccgaa
     7801 accatttccc caattgccgc ccttatgaat gatccgccac tccccgatcg gagtgggagt
     7861 atccggctcg ccgacagcca ccggatatct ccggaaaacc ttaccgtccc gataaagagt
     7921 caagtgcaat tgcttggtat cgatgtttat caaatcgccg ttccctaccg gattattggc
     7981 agtagtgata tcccgttcca tcagggtaaa ctcatcacca tcttcgcacg tcaccttggt
     8041 ctggaccgtg ttgttgccaa tgatccgtct aagccctatg aaaaacgcat ctttccgatc
     8101 ctttttaagc aacatctccc ggagcgcgct ttgccgaact tgaaagatgc cggaacgaaa
     8161 ctggagactg acggtcatga cgatcaatat taccgcccaa agaatgatta aaagtttaga
     8221 aaatgacttc ccggtcatcc ggattgcacc gccccgttac tccaaaatga catccgctaa
     8281 tctttatgac ttgagactac acattatgat tggggcggta agaaatgata agaaacccgg
     8341 tgtccttcct aagggcaccg ggtatagtag gataaatcgc gattaaaatt ctacatcgat
     8401 ctcgacgcac aaatggactt tcgtatttga tcctgcagac acattaatat ttgacccgtt
     8461 ttccagtaac aatatgaatc gtcgcctctc cgatattggt ggcgtgatcc ccggcccgtt
     8521 caagccactc ggcgatcaat agaaaacgtg aagcttggta ggccttacgc gggtcggact
     8581 cctgaccggt gatccggatc aactcctccc ggacctcctc atagagatta tcggcttcat
     8641 cgtcccgctg acaaaccgca tcggccaggt cggcattttt ttcgatgaac gccttcaatg
     8701 atgtattgac catattcatc accagattcg ccatctgagg gatgtgaacc aggggtttta
     8761 gataggattc atctttcagt tccatgacta cctcggcgat gttggtggcg taatctccga
     8821 tccgctccaa gtcaaccccg atcttggcca tggccaaatc gcggcgcaag tcgaaggttg
     8881 tcaacgaagg attcctgttg gtaccgatac cccgttcaat ctcttcctca acgacgatct
     8941 gtaaattgtc gagttgatca tcctgttcaa tgacttttaa agccagcttt acatcctgat
     9001 cacgcagtgc ctggatggcg agctttaaag attccgcagc caaattaccc atctttaaca
     9061 gatgttgcgc agtttgatcc ccgacctgtt cagatatgtc ggtcatttcg cttcactcct
     9121 ttttcatttt tattagattc cctaagaaac accccgggca gcaaactgtc cgtgggaact
     9181 gccaaaatcg ttagcccatt gcctttgact tgtctagcgc attgatcccc cagactccga
     9241 tgagaatcaa ggaaccgccg accaaatggt aaggtccgag agcctccccg cggaagataa
     9301 caccggcggc caccgaaacc acggtagtta aattgttgaa ggccgccacc ttggccacag
     9361 ccattttttt caacatataa ttcactagaa aaaaggcgcc gaccgaagag agtagtccca
     9421 aataaatcag ggggaataaa gatccaatcc gggtgaaccc ctgaaaataa aaccctttgg
     9481 caggaaaagc cgttagccgt aacagattaa aaaataccgc ccccgcatac atcattccgg
     9541 tggttatctc cagtggcgaa taagtggctg agtacctgcg ggataagatg ttatataccg
     9601 cagcagccgc tacagccaga agcaggatag ccatacccgc ggtatgaact gccaaatttg
     9661 ctccggaacc ggcgagtaca atcattccaa cgccggccac ggagcataaa acaaacagcc
     9721 actgacgcca tccggcctgc tcgtggagga agagcccgcc gagtatcgtg acagccaccg
     9781 gaatcaacgc aatcaccagg ctcgactcgg acgccgaagt gagctgtatt ccaaaggttt
     9841 cgccgagaaa ataaaggatg ggttggaaga gtgtcaacgg aagaatgccg gcaaatctct
     9901 ttagattcaa acgaatccgt acgaccccgg tgagattgag cagaaacaaa aatatggccg
     9961 ccagtgtaaa ccggttggcc aagagttcaa tcggagacat caatgaaagg gaatttttgg
    10021 tgaataaaaa ggagagaccg aagatgagag atacaacaat tccggcgatc accgctaaca
    10081 actgatcgga tttggtcaag tcgtcacatc ctatcgggaa gattaggcgc gattgcggat
    10141 ttccggtaat cccatccccg cgcgcttagt tatcagatag gccccttcca cataatcagc
    10201 gcatcctcat tattatccag ataatactta cgtcgaaccc ccatatgaac aaagccgagt
    10261 ttctggtaaa gatcttgagc cgccaggttc gttcggcgaa cttccaaggt taaatgacgg
    10321 acgtcccgtt tccggaccgc ttcaaccaac gcaagcatca aggccttccc caccccttga
    10381 cggcgataac ccggatgaac agccagatta gtgatatgac cttcgtcaaa gatcacccat
    10441 attccaacgt aaccgatgac attccccagc ggtgacttgg cgactaaata tatcgccctt
    10501 tcgttctcaa gcaactcgta aagaaacgaa ttacgggacc agggcgtcgg aaacgataga
    10561 tcttcaatct tcaatacttc ttccaaatct tcgactcgca tcgggctgat ctccggttgc
    10621 attcaacccc tcctccaaac gggcctccgc ttccactttt ccgagataaa agggggtcaa
    10681 ttccgataaa acggaggtac gacgatccca atcacgaaaa gcaattgccg ccaattttcc
    10741 ggccgagggc ctggcccact cttccggacc tatcattaat ccaggaacca gttcccagat
    10801 agacctatat tttttacaac cgtcgccgca gattacggtt ccggaataag ctgccagttg
    10861 tcgggcaaat tcggttgctg ccattacttg aggcattttg agacattcta agatttcccc
    10921 atcgcttttg cggtataaag cggcataaac tctttcacgc cgggcatcca aaactgtggc
    10981 tatgtacgta accggaatat agcgcgcgtt ctcggcaatc acccgtaaag tgctcaggcc
    11041 gaataccggt cgctcaatgc ctgccgcgat acccttcacg gcagccaatc ccactcgaat
    11101 tccggtgaaa gagccgggtc cggtgcctac ggcaatcaga tcgatcttcc ggaattcacg
    11161 gccggatttt tgaatcatcg tgcctagaag atccagtaga tattctccgc cgcccttccc
    11221 ggtttttaac cccgcctcag ccacaacctc tccgtcttcc cagagagcca gggttcccca
    11281 gggagttgcc gtgtcaagac ccaagatgag catggcttaa actctccaaa aaatgatcat
    11341 attccggtcc ttttgagttg attgtaacaa tcctgcccgc cccatccgga gcgaactcca
    11401 tgataatctc gagataagtg ggcccaagcc gttctaacag caattgcccc cattcaatga
    11461 ccatcactcc ggtttcggca aggacctccg gctccaggat atccagttcc agactgctct
    11521 taagccggta aagatccaaa tggtaaagag aatatcgccc ctggtagctt tttagtaatt
    11581 gaaacgtagg gctggtaacg gcagccgtca ctcccaatcc gtatgcgatt cctttggtga
    11641 gggtcgtttt tcccgcaccc aactccccaa ataaaaaaag gagatctccg ggtttaacca
    11701 gctctcccaa taatcttccc atctcgtatg tcaattcggc tgttttcagc gtcaaggaaa
    11761 tactcttcat gggttaccct gctttgattt tttttgaacc cgcgtgcgcg attacctgtc
    11821 cccggatgcc tgtcggtaaa atcaccctat gccgcaagcc ccggggataa aacacttctt
    11881 ccttattatt tagcttacca tgttcaacca gtttggtcaa gtctcgccga tccgacaata
    11941 ctgaatgact gattcttcaa taagtctcta tatagcgcga ttttaaaaaa agaaaggccc
    12001 ttttatagag aggccccaat actaacttaa cgcttttaac cccagactga ttgcaatggc
    12061 ctcgtccact cgggccataa tctcctcgtc aagtcgggtt attctttctc tcagcctttg
    12121 tttgtcgatg gtccgtaatt gttccaaaag gatgactgag tccttctcaa gctgatatcg
    12181 ggcggcactg atttcaatat gggttggaag tttagcgcgt ttaatcttgg aggtgatggc
    12241 tgcgataatt gtggtaggac tatacgtatt gccgatatcg ttttgaacga ccagtactgg
    12301 cctgagaccg ccttgttccg atccgatcac cggatttaga ttcgcataaa agatatcgcc
    12361 acgacggatc gttgaaacca cgacgttaat ccctccgcgt gcgagtgccc gcgtttacag
    12421 agtgcgcgcc tgcgcgcaag taattctcat attgctccag atcccggagg tcctcgtcga
    12481 gtccgtcttc ggccaaagcc aggttaagct taccggtttt ttcgtagccc agccgatatc
    12541 tctccatctg ttcgtgatgg cgccgctccg cgatgagcag caacatcgct tcccgaacaa
    12601 aggcactgcg atttcccgtt tcccggcgga cgatcccatc cacctcttgg aggagactat
    12661 ccggcaaact gaccataatc cgtttggcct gtgccataaa tccacccccg agaatcagaa
    12721 ttttaaatta cctcctgcta tcgggcgggt tcacttggtt accaaaagcc ctctgcttat
    12781 aaccaataaa gtccattccc tgataggtct tcctgattta ttatatacag tttctcctaa
    12841 ttcagtcaaa tgggagtctg tcttcggaac ctgacagaac ggatgaggaa tccgttccct
    12901 taagaattgg tttccttaaa actgatcttc tttaaagctc actatcaata aaatgccggt
    12961 cttcattgtc actctggcgg ttgccgcggt aaattgatta tgaatccccc gggtatgaga
    13021 aaaatataag gtatcattct gaagtggaaa cttaagtccc gtggtcgtca ccccagtaac
    13081 ccggttagtc cagggaatga gtgaaacagc ccatcccggc tttgctgtca aaacaatatc
    13141 tcgactcgta gcagtaatct catgggtctc atctattaaa taagcttcaa ccccatgttc
    13201 tagagagtta actaataatc ccagattacc tagggtatga tcaaacctac cgcccaaacc
    13261 tccaaaaatc cgaatccgca aggctccggc tttcaaagct tgacctacga ccaattctaa
    13321 atcggtctca ttttttgctt ccggatattt ttcaatctgt accccagctg ccgctaaccg
    13381 actaagatcc gattcagcaa aagaatccat gtccccaacc agtagatccg gcattacccc
    13441 tagctctagt agataggttg cgcctcgatc tgccgcatat aatatctcgg gacgtatcgt
    13501 caattcctgc ttcaactgtt tcaaactaac cggaccgccc cctaccagga tcgcctgttt
    13561 catggaaacc ctcctccttt atggtccttt ttcgtgtgtt tcgtgccttt cgtggtgaaa
    13621 agacccttcg ctttatcgtt cctctgaaaa aacgatttga ggacctaaac tacgaaacac
    13681 acgatattca cgaaaacata tatcggtttc tttgatcctt tttcgtgtgt ttcgcgcctt
    13741 tcgtggtgaa aagacccctt cgctttatcg ttcctctgaa aaaccgattt gaggccttaa
    13801 actacgaaac acacggaatt cacgaaaacg tataacggaa ttcacgaaaa aggctataaa
    13861 aaaaacacga cccgaggtcg tgtttttgag gttaacgttt ggagaattgc ggcgctttac
    13921 gggccttctt caaaccgtac ttacggcgtt ctttcatccg cggatcacgg gttaagaatc
    13981 cggctttctt tagcggcaga cggaattcgt cttccacccg gagtaaggcc cgggcaatac
    14041 catgtctcac cgcaccggcc tgaccggaga tacctccgcc atggacgctg gcataaacgt
    14101 catactttcc ctccgtagcc gttgtggcca tgggttgtct gacagtcagc tccaatactt
    14161 tccgcccaaa ataatccatc accggtttcc cgttgacgat cacatttccc tgtcccggaa
    14221 ccaatctaac tttggcaatg gcgcatttgc gtctcccggt tccgtaatac atcgattttg
    14281 tagttttctt cgcacgcggc atttagtatt cctcccttct caggccttga tctcgagttt
    14341 ttccggaact tgagcttgat gcggatgctc gttgccacgg taaactcgga gttttttgat
    14401 cattttccgt cccatactgt tatgggggag cattccccaa atcgtacggg taagtgcgta
    14461 ttcaggcttg ttctttaaaa gctgaccgta cttgatggct tttaaaccgc ccggatacat
    14521 ggaatgggta taatggatct tgtcttccgc ttttttgccg gtgagaacta ccttttcggc
    14581 gttaatgatg atcacgtggt caccgcagtc gacattcggc gtgaaggtgg gtttgttctt
    14641 accgcgtagg atcatagcca cagttgaggc taagcgtcct aaagtttggc cttcggcgtc
    14701 aaccagatac cacttatgtt gaacctcctc aggcttggcc atataggttt gcatattcat
    14761 ctctccccct gcataaatgc tgttgatctt ccaatcggac ccctataaaa tccgttcgga
    14821 aaaaaagggc tctattttt
//
LOCUS       DASVMB010000316         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_703499,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000316 DASVMB010000000
VERSION     DASVMB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703499"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VF531_09020"
     CDS             <1..765
                     /locus_tag="VF531_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714152.1"
                     /translation="ETENLTVFSLDLHHPALTQNGRILVDIDGYAVPADAGSRAEFVY
                     SPTRNCFVWQPERRSGLASHQGGGLADLVTDPIFIVYGTRKPGRVEILKKSAAILADW
                     STGPVVSFGTKVGRFRVKPDTAVTKADLRGANLILLGTVAENRLTAKYAAAKQMPVKF
                     DSKTVTVQGKKYPGAGFILVYPNPEASGRLLGILSLPFNTKTLEQFMVSLNLSLRQYA
                     PGNGVGSFTTPDLMVLKSPGELLWSGSFDRNWRFRP"
     gene            1043..2461
                     /locus_tag="VF531_09025"
     CDS             1043..2461
                     /locus_tag="VF531_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005494030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX7714153.1"
                     /translation="MIKFGTGGWRAIIGEDFTKDNVQLLSQAVAELIKTELCANGGFV
                     IGYDRRFLSDKAAKWIAEVLAGNGIKVYFIDKVAPTPLVMFTVKNLQVPYGAAVTASH
                     NPADYNGVKIFTKGGRDATEEVTGKLESFIEKCLGTGKKIRSLPFEEALQEQRIEIID
                     PFNEYIDTILGMIDTEAIKNQKLKIILDPMFGVSKTCLQTILLTARCEVEVINDRHDT
                     LFGGRLPSPTTHTLQRLRDLVVEKGYDIGIGTDGDADRLGIIDEKGDFIHPNNILALL
                     YYYLLKYKGWKGGVVRNVATTHLLDRIARDFGEECYEVPVGFKYISSMMEEKNALLGG
                     ESSGGLTIRGHIKGKDGVFAASLLIELLSVTQKSLSELLEEINGRYGYFYMVENDFRF
                     DAVEKARLMQRLFEEKAMPRFHESIAKVSYRDGLKVYFENGGWIIARFSGTEPLLRIF
                     TEMSNEADAAAISDQMKAFLNL"
     gene            2492..>3840
                     /locus_tag="VF531_09030"
     CDS             2492..>3840
                     /locus_tag="VF531_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HEX7714154.1"
                     /translation="MKYGYFDNTNREYVIERPDAPVSWTNYLGVRDLCAVISHNAGGY
                     AFYKQAQHHRMTRFRPNGVPLDRPGHYVYLRDDETGEYWSLSWQPVGKDLTKAKYQCR
                     HGLSYSRFSCDYQGIAAEQILFIPVDDDVELWDVRIQNHSGGPRKLSVFSYVEFSYHH
                     VEIDNQNFQMSLYASGSSCRDGIIEYDFFYEPQTFHYFASDFEPDSYDCLRDKFIGSY
                     RSETNPVAVERGSCSGSSELGGNHCGALHKRLVLKPGEMVRVLFLLGVGNREAGTAMK
                     RKYSNPANVDRAFQDLKQYWDQKLAAFQCHTPHEGLDTMINTWTLYQAETCVVWSRFA
                     SFIEVGGRTGLGYRDTAQDIMSVPHTHPAKVRQRIAELLRAQVQMGYGLHLFEPEYFD
                     PDRPPAPQFKSPTVVPEPDRASLIHGLKDTCSDDALWLIPSICEYVMETGDVGLFDQV
                     "
BASE COUNT          965 a          908 c         1024 g          943 t
ORIGIN      
        1 gaaaccgaaa atcttaccgt attttcattg gatttacacc atccggcgtt aacccaaaac
       61 ggacggattt tagttgatat tgacggttat gcggttccgg cggatgccgg cagtcgggct
      121 gagttcgtct attcaccgac ccggaattgc tttgtctggc agccggaacg acgttcgggg
      181 cttgcttcgc atcagggggg cgggctggca gatctggtaa cagacccaat ttttatcgtc
      241 tacggaaccc gtaaacccgg acgagtggag attctcaaaa aatccgccgc gatcctcgcc
      301 gactggagca cgggtccggt ggtttccttt gggaccaaag tcggccgttt ccgggtaaaa
      361 cccgataccg ccgtcaccaa ggcagatctt cgtggagcca atctaatctt gctggggacc
      421 gtcgcggaga accggcttac tgcgaaatat gcggctgcga aacagatgcc cgtcaaattt
      481 gattcaaaaa ctgtaacggt acaaggcaag aaatatccgg gagcaggatt tatcctggtt
      541 tatccgaatc ccgaggcttc gggaaggtta ctgggcatcc tatctctacc ttttaatacg
      601 aaaaccctgg agcagtttat ggtcagcctg aatttgtcat tacggcaata tgccccgggc
      661 aatggcgtcg gcagttttac cacaccggat ctgatggttt taaaatcccc cggtgagtta
      721 ttgtggtccg gttccttcga tcggaactgg cgtttccggc cgtaaggatc tagtttattg
      781 catattgaaa aaaataagca ggaaaaacat gggctatggc gaataaaaca atatcaagca
      841 aaatccctca atcaaaaatg accttcggaa ttatttcata acgggcaggg accgataacc
      901 aggatttatg ctaccgggga ttggaatgat cttgtccata taagggtttg atctttagtt
      961 ggggtttatt aagaatcaat agtttatttc atagtgaaaa aatgtagcgt tttcagactc
     1021 gcgtcacaaa ggggaatcag tgatgattaa atttggtact ggtggttgga gggcaatcat
     1081 cggcgaggac ttcaccaagg ataacgtgca actcctttcg caggccgtcg ccgaactcat
     1141 caaaacggaa ttatgcgcga acggcggatt cgtgattgga tatgatcgcc gatttttatc
     1201 ggataaagcc gccaaatgga ttgcagaagt cttggcaggt aacggaatca aggtttattt
     1261 catcgacaaa gtggctccaa cccccctcgt catgttcacg gtgaagaatc ttcaagtccc
     1321 atacggcgca gcggtaactg ccagccataa ccctgccgat tataacgggg ttaaaatctt
     1381 taccaaagga ggccgcgacg ctaccgagga agtaaccggt aaactagagt cattcattga
     1441 aaaatgcctt gggacgggga agaaaatccg atcacttcca tttgaggaag ctctgcagga
     1501 acagcggatt gagatcatcg acccgttcaa cgagtacatc gacaccatcc tcgggatgat
     1561 cgacacggaa gcgattaaga accaaaagct caagatcatt ctggatccga tgttcggtgt
     1621 atccaagact tgtttgcaga caatcctgtt gaccgctcgt tgtgaagtcg aagtgatcaa
     1681 cgatcggcac gatactttgt tcggcggcag gctgccttct ccgaccaccc ataccctgca
     1741 gcgtttacgg gatttggtag tggagaaagg ctacgacatt ggaatcggaa ccgatggcga
     1801 cgccgatcgt ttggggatta tcgacgaaaa gggcgatttt atccatccca acaatatact
     1861 ggccctatta tattattatc ttttgaaata taagggttgg aaaggcggag tggtcaggaa
     1921 cgtggccacc acccatctcc tcgaccggat tgcccgggat ttcggggaag aatgttacga
     1981 agtaccggta ggatttaaat atatcagttc catgatggag gagaaaaacg cgctgctggg
     2041 tggggagagt agcggcggtt tgaccattcg cggtcatatc aaagggaaag acggtgtttt
     2101 tgcggccagt ctgttgatcg agctcctgag tgtaacccaa aaaagcctct cggaattgct
     2161 ggaagagatc aacgggcgat acggttactt ctatatggtg gagaacgatt ttcgtttcga
     2221 cgcagtggaa aaagcccgac tgatgcagcg gctcttcgag gaaaaagcga tgccccgttt
     2281 tcatgagtct atcgccaaag tttcctatcg tgacggcctt aaggtatatt tcgaaaacgg
     2341 cggttggatc atcgcgcgtt tctccggaac cgaaccgttg ttacgaatct ttaccgagat
     2401 gagcaacgag gccgatgcgg cggcgatcag cgatcaaatg aaggcgttct tgaatttata
     2461 agcgccacca actttagagg ggaagatcat aatgaagtat ggatactttg ataatacaaa
     2521 tcgggaatac gtcatcgagc ggccggacgc cccggtttcc tggaccaatt acctgggagt
     2581 cagggatctc tgcgccgtca tttctcataa tgccgggggc tatgcctttt ataaacaagc
     2641 tcagcatcac cggatgaccc gcttccgtcc caacggagta cccctggatc ggccgggaca
     2701 ttatgtttat cttcgtgacg atgaaaccgg ggagtattgg tctctttcct ggcagccggt
     2761 ggggaaagac ctgactaaag ccaaatatca atgccggcac ggactttcct attccaggtt
     2821 ttcttgtgat tatcaaggaa ttgccgcaga acagatcctg tttatcccgg tagacgacga
     2881 tgtggaactt tgggatgtcc ggatccaaaa tcatagcggc gggcccagga aacttagcgt
     2941 attttcatat gtagagttct cctatcatca tgtggagatc gataatcaaa atttccagat
     3001 gagtctctat gccagcggtt ccagctgccg ggatgggatc atcgagtacg atttcttcta
     3061 tgaaccgcag accttccatt acttcgcctc cgattttgaa ccggacagct acgactgcct
     3121 gcgggacaaa ttcatcggta gttaccggag tgagaccaat cctgtcgctg ttgagcgggg
     3181 gagttgttcg ggaagttccg agttgggcgg caatcattgt ggcgcgttac ataaacgcct
     3241 ggtgttaaag cccggggaaa tggtccgggt cttgttcctc ctgggtgtgg gaaaccggga
     3301 ggccggcacg gcaatgaaaa ggaagtattc caaccctgcc aatgtcgatc gagcctttca
     3361 agatttgaaa caatattggg accagaaatt agcggccttc caatgccata ctccccatga
     3421 ggggctggac acgatgatca atacttggac gctgtatcaa gctgagacct gcgtggtctg
     3481 gtcgcgtttt gcgtccttta tcgaggtggg cggcaggacc ggtctgggtt atcgcgatac
     3541 ggcccaagac atcatgagtg tgccccatac ccatcccgca aaggtccggc aaaggatcgc
     3601 ggaattactt cgggctcaag tccagatggg ttacggcctc catcttttcg aaccggaata
     3661 tttcgatccc gatcgtcccc cggcgcctca gtttaaatca cccaccgtgg ttccggaacc
     3721 ggatcgggcc agcctgattc acgggcttaa ggatacctgt tccgacgatg cgctctggct
     3781 catcccttcc atctgtgagt acgtcatgga gaccggggat gtcggccttt ttgatcaggt
//
LOCUS       DASVMB010000317        11574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_261102,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000317 DASVMB010000000
VERSION     DASVMB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261102"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF531_09035"
     CDS             complement(<1..264)
                     /locus_tag="VF531_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEX7714155.1"
                     /translation="MRNQWFVIVNPCSGSGKGAKDWPRIASLLGDSGIPFEAVFTQRP
                     NHATELAGQAVRKGWHRLIAVGGDGTVNEVVNGIFGQKATATRE"
     gene            398..715
                     /locus_tag="VF531_09040"
     CDS             398..715
                     /locus_tag="VF531_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714156.1"
                     /translation="MSSFKFGINIGKGETAKIRIKINISRISDEIPKKTDARLPDTDP
                     GSITTLPLPFPQLSDEPGPLENVSIPKGQSGFWQQLFSGFTTDKPINNRFFTLLKTSG
                     KKK"
     gene            complement(726..1646)
                     /locus_tag="VF531_09045"
     CDS             complement(726..1646)
                     /locus_tag="VF531_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714157.1"
                     /translation="MKQKPMVIIAVFIAGLLSLSFIAFINNGLHLAKEKAMVDGFKVL
                     MAKEEVTPVELIQYMDKNIALVSRQDAATFLMGLERIQKVNLPKWQEKFENEVLQEKM
                     ARIYRDNGWTLTDLSGIQDGDLKAIVSGAISNGFKVETAEGFFFPVIDYAFLKKYRAV
                     LPPDMVAYIDIMAEESDKTPVKDAGLMIGWEEVVKRALCQEKFIKEYGSSAQVRAVQQ
                     LLNRYLTFALFGTNNTPLFSYETQRMVPKAKQIYQQYTWNNENGRFSALMKEYLSVLK
                     ENDFRLTSEVDTYRKKAESIFSDNSMKRKE"
     gene            complement(1876..3354)
                     /gene="cysS"
                     /locus_tag="VF531_09050"
     CDS             complement(1876..3354)
                     /gene="cysS"
                     /locus_tag="VF531_09050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7714158.1"
                     /translation="MKVYNTLTRCKEEFIPREPGQVGIYVCGVTPYNFAHIGNARPPV
                     FWDVVRRFLKYRGYQVKYIQNFTDVEDKIIARANQLGREPLDLAAEYARIYLEDLATL
                     GVMPADRYPKVSEHIPEIIEMVRVLIAKGHAYELNGDVYFEVGGFKDYGKLSGRSLEE
                     MQAGARVEVNEAKRDPMDFALWKAAKPGEISWESPWGPGRPGWHIECSAMSLKYLGNG
                     FDIHGGGEDLIFPHHENEIAQSEGFSGTSFVHYWMHNAFITVGGERMGKSLGNFSTIR
                     QVLAKFPPRVIRLWFVGTHYRNPISFGDTELTAAARGLERLDNAEFNWEHLIQSEAVP
                     GDETDKLTPIKAAIAGTEEQFVAAMEDDFNTPQALASLYDLVREVNRFTQGNNFLLTR
                     GSQTVIQEALDKLTGFEEILGIGSQLFTATASGSGVNHGAAQAHQETPGLDQEAIEAL
                     IAKRTQAKKEKNWALADEIRDELKAKGIMIEDTPQGVRWRKV"
     gene            complement(3374..4042)
                     /gene="cysE"
                     /locus_tag="VF531_09055"
     CDS             complement(3374..4042)
                     /gene="cysE"
                     /locus_tag="VF531_09055"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX7714159.1"
                     /translation="MFQRIREDIHTVFERDPAAKSRLEVLLCYPGVHALMAYRLSHWF
                     YQKRWFLLARVISQITRFFTGIEIHPGARIGRRFFIDHGAGVVIGETTEIGDDVTLFQ
                     GVTLGGTGKEKGKRHPTVGNNVMIAAGATVLGSMKIGDNAKIGAGAVVLQEVPPNTTV
                     VGVPGRIVVQDGVRVRHGVDLAHNQTPDPVAKMIECLQRTVIELENRVEELERTNAVS
                     SRIK"
     gene            complement(4462..5358)
                     /locus_tag="VF531_09060"
     CDS             complement(4462..5358)
                     /locus_tag="VF531_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEX7714160.1"
                     /translation="MISTKRWWVIGIVIGLLVVSSAATQPQRVVTYGADLTPEEEVIV
                     FRDFPLPQGIGPKDIKSIKVTNEEEWRLFEGLVPAEQIGTKSVSSVYLEKLEPGAGIK
                     VETKNLTYITPHIFANTLATAGISDARVYATAPQPVSGTAALTGIYKSFETLTGSSIP
                     SGTKRIGALELIDTGYLGEKVGPEQAAILVERCKERLLSVRITTKTEALRIVDQGVRD
                     QNLKLTPEEKSELADLLLKIGSLNPDLTRLKTQLRNFTQRQNETQPQTAPQSFLSRLI
                     AYIQSLIKQLVSFVGRIGSGFR"
     gene            complement(5757..6800)
                     /locus_tag="VF531_09065"
     CDS             complement(5757..6800)
                     /locus_tag="VF531_09065"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEX7714161.1"
                     /translation="MEFNREDHPHRRRNPLTGAWVLVSPHRTKRPWTGQVEKPAPEDK
                     PEYDPGCYLCPGNVRAGGVKNPNYTGTFTFVNDFAALLPDVPAGGNDDFFFKTESVRG
                     ICKVLIFSPSHRLTLPLMSPEDLLKVAEMWRDQYLELSALPDIKYVQIFENKGAMMGC
                     SNPHPHGQVWASQSIPDELVKKCASQAEYIQSHGRPMLLDYAEVELKREERIVYVNDG
                     FVVLVPFWAVWPFETMIVPRKQLASLADFGPADLRLLADAVRVLTTKYDNLFETSFPY
                     SAGMHQSPCDGREHPGFTWHMLFYPPLLRSATIRKFMVGYELLAEPQRDITPESSAKR
                     LRELSDRHYLLKA"
     gene            complement(6940..8466)
                     /locus_tag="VF531_09070"
     CDS             complement(6940..8466)
                     /locus_tag="VF531_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7714162.1"
                     /translation="MENLENAELMLYAENENRVNKIHIIVSSAILLIGDLFALVAKLL
                     MHDNTFSYMVLVYVTGSGLVFLGYPIYLMFKNPYHKAIKYLMTFCEIIVIAIVNLTMG
                     RANPDTFLNFFWGICLSVLYFNPLVTAVYGTGSLFAYASLMFFVPELHPQVAGATSVV
                     IKRGMYMLFVALAGVAAAVIANQLMRRLGQKERQNKESLVKIEKLLQEIGQSSQSLST
                     SSQQLSASAEEMSAQTEELNASFATVSNEAIQNQHEMEAAQEYLADFSEKTVEKSDLT
                     QKTISMVESISQSALNGIDDSTAVKTAVHSFRSQFSKNTAVFDGLLQEINSVAKISQT
                     LTSISKEINLLSLNASIEAARAGEFGKGFTVVATRVRKLAEETKQALDNVNEITKKIA
                     GSIDSAETESRNAGEVLETTIQSINTMTEKFSAIVDELNSSIPLLKEVGAFLSSQSAM
                     VNQVGTKITEATSFSKESTQTMTNLTEVVNGLAAMAQQIAASSEELAGLSETLASEAQ
                     "
     gene            complement(8481..9236)
                     /locus_tag="VF531_09075"
     CDS             complement(8481..9236)
                     /locus_tag="VF531_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7714163.1"
                     /translation="MYCTVNDVKIHYQVLPKDGAPTLLLMHGAGTRLNILKPLATKLS
                     KYNRVLIDLPGHGKSAGRPLGSVAEYADFIEEFVRTLKLKDIYLVGYSMGGFIAIELA
                     IRKKTPLKGLVMVDTTPQMEKNLEDSFKEQIRRDEFDEFHFLSYGGIWNWNVFRIVVT
                     SLFYSLTHKPVMLADFQNCVLRFDRRQEIDQIQLPALIITGEKDPIAPPVDAEFCRNR
                     ISGAEKEIFPRYGHFLPVAIPKAVARRIEKFIK"
     gene            complement(9532..10917)
                     /locus_tag="VF531_09080"
     CDS             complement(9532..10917)
                     /locus_tag="VF531_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7714164.1"
                     /translation="MLEFNKHSNTLEQSEYIYALQDVTEPHLYRYLFNYEEVPKVPFN
                     HRHVPMRPPEKIWITDSTFRDGQQAREPYTVKQIVDLYKYLHRLGGPKGVIRQTEFFL
                     YSNKDKEAATQCMELGYDYPEITSWIRAVKEDFLLVKEMGLKETGILASCSDYHIFKK
                     LKLTRKQAMEQFLSIVKSALEIGVTPRVHLEDITRADFYGFVVPFVLELMKLQEESKI
                     KIKIRACDTLGFGVNYPGVALPRSVPGIIYGLWHHAGVPSDQLEWHGHNDFYKAVSNA
                     GTAWLYGCSGVNCTLLGIGERTGNCPLEAMIFEYASLRGSLDGMDTTVITEIADYYNK
                     EIGYQIPPMTPLVGREFNFTRSGIHADGLLKDEEIYNIFNTEKLLNRKVVVAVNEHSG
                     AAGLAHWVNSYFNLRDDRKLEKRDSRLLKIKGWVDEQYQNGRNTVIGDHELVEFIQAE
                     TPDLWEELNRR"
     gene            complement(11221..>11574)
                     /locus_tag="VF531_09085"
     CDS             complement(11221..>11574)
                     /locus_tag="VF531_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714165.1"
                     /translation="SGNVNPFVSLIATYLTAILISLAILPFYRQGPGIGESIKSLNWA
                     SIVLGVAIIGLELGYLLAYRMGWQISLAGVFSNVAVALLLIPIGVLLFREHLSGLNVL
                     GLAFCVLGLILTNIK"
BASE COUNT         2920 a         3100 c         2587 g         2967 t
ORIGIN      
        1 ctcccgggtg gccgtagcct tctggccaaa aatcccgttt accacctcgt tgacagtacc
       61 gtcgccgcct acagcgatca ggcggtgcca acctttacga acggcctgtc ccgcaagttc
      121 agtggcgtgg ttaggtctct gggtaaatac ggcttcaaat ggaatgccgc tatcccccaa
      181 taacgaagcg attcgcggcc aatctttagc tcccttgccc gagccggaac agggattgac
      241 aataacaaac cattggttac gcatcgtgtg ctcctttacg atcaccctta ttcgccttaa
      301 aaaggagatc tcctgcaaaa atgtgccgtc atcccggagc cattcgcctc ccccggaata
      361 ttctttagag ataggtgatg atcaagagga gtttcaaatg agttcattca agttcggcat
      421 caatatcgga aaaggtgaaa cggctaaaat ccggatcaaa attaacatta gcaggatttc
      481 cgacgagatt ccaaaaaaaa cggatgcaag gctgccagat accgatcccg gatctataac
      541 tactctgccg ttgccttttc cccaattgtc cgatgaaccc gggcccctgg aaaatgtgag
      601 tattcccaaa ggccaatccg gtttctggca acagcttttc tccggtttca caaccgataa
      661 accgataaat aaccgctttt ttacccttct aaaaacctcc ggaaaaaaga agtaagcagt
      721 aatgctcact ccttacgttt catactattg tccgaaaaaa tagattcggc ttttttccta
      781 taggtatcca cttcactggt cagccggaag tcattctctt tgagtacgct gaggtactct
      841 ttcattaacg ctgagaaacg cccattctca ttattccatg tatattgttg atagatctgt
      901 ttggccttgg gtaccatccg ttgggtttca tagctgaata acggagtgtt gttggttccg
      961 aacaaagcaa aagtaaggta acggttcaac aactgttgca ccgctctaac ctgcgccgag
     1021 gagccatatt ctttgatgaa cttttcctgg cacagcgccc gcttaacaac ttcctcccaa
     1081 ccgatcatca acccggcatc cttcaccgga gttttatcgg attcttcagc cattatatca
     1141 atataggcga ccatatccgg cggtaaaact gcacggtact tcttaaggaa agcgtaatcg
     1201 atgacaggga agaaaaaacc ttcggcggtt tccaccttaa aaccgttact aatggcgccg
     1261 ctcacgatcg cctttaaatc gccatcttga ataccgctga gatcggtgag ggtccagcca
     1321 ttgtcccgat agatcctggc catcttctcc tgaagcactt cattttcaaa cttctcctgc
     1381 catttaggca ggttaacctt ttgaatcctt tcaagtccca tcaaaaaagt agctgcgtct
     1441 tgccgggaaa ccagcgcaat gtttttatcc atgtattgaa taagctccac cggtgtaact
     1501 tcttccttgg ccatcaagac cttaaagccg tccaccatcg ctttttcctt ggctaggtgc
     1561 aagccgttat tgatgaatgc aataaatgat aatgagagta atccggcgat gaatactgcg
     1621 atgattacca tgggtttttg tttcatgttt atccccctcc atcattatga cgccctagaa
     1681 cttggatcaa aaaggccggg tcactccgac ccaatttgca tccgatttgt tcattgttga
     1741 ttgcaaacgg gtcaatagca gcctggcctt tgtatctgtt ttcattcatg gattgttggt
     1801 tccaggtata tggaaagaaa agtaactcaa ccctttccac agacctttac ccatccactt
     1861 tagtagcggt tgatcttaaa ccttccgcca tctaactccc tgaggagtat cctcgatcat
     1921 aatccctttg gccttgagct catcccggat ttcgtctgcc agtgcccagt tcttctcctt
     1981 tttagcctga gttcgtttgg caatcagggc ttcaatggcc tcctgatcca ggcccggggt
     2041 ttcttgatgg gcttgggcgg ccccgtggtt taccccggaa ccggacgccg tggcagtaaa
     2101 gagttgactc ccgattccca atatttcctc gaaaccggtc aacttatcaa gagcttcctg
     2161 gattacggtc tgagaccctc gggttaacag aaaattattc ccctgggtga agcgatttac
     2221 ctcacgtacc aggtcataga gactggccaa agcctgggga gtgttgaagt catcctccat
     2281 cgccgccaca aactgctcct cggttccggc aatggctgcc ttgattgggg taagcttatc
     2341 cgtctcatcg cccggaaccg cttcgctctg tatgagatgc tcccaattga actccgcatt
     2401 atccaaccgc tccaatccgc gggctgcagc agtcagttcc gtatctccga aactgatagg
     2461 gtttcgataa tgggtcccga cgaaccaaag cctgatgacc cggggcggaa acttggccaa
     2521 gacctgacgg atggtggaaa agttccccaa tgatttgccc atccgttctc cacccactgt
     2581 gataaaggca ttatgcatcc aatagtgaac gaaagaagtc ccggaaaaac cttcggattg
     2641 agcaatctcg ttctcgtgat gcggaaagat caaatcctcc ccgccaccat ggatatcaaa
     2701 accattacct aaatacttca aagacatggc agaacactcg atatgccatc ccggacgtcc
     2761 cggaccccag ggggattccc aactaatctc gccgggcttg gccgccttcc atagggcgaa
     2821 atccatcgga tcgcgtttag cctcattcac ttctactctg gcgccggcct gcatctcttc
     2881 aagcgaccgc cccgatagct tgccgtaatc cttgaaaccg ccgacctcaa aatagacatc
     2941 cccgttcaac tcatatgcgt ggcctttcgc tatcagaacc cggaccatct caatgatctc
     3001 cgggatatgc tcggagactt tggggtatcg atccgcaggc attaccccga gagtcgccaa
     3061 gtcctccagg tagatccggg cgtattccgc ggccagatcc aaaggttcac gccccaattg
     3121 gttggcgcgg gcaataattt tgtcttctac atcggtaaaa ttttggatgt actttacttg
     3181 ataaccgcga tattttaaaa agcggcgtac cacatcccag aaaaccgggg gccgggcatt
     3241 tccgatatgg gcgaaattat aaggagtcac tccgcagacg tagatgccga cctgtcccgg
     3301 ctccctggga atgaactctt ctttgcatct ggttaacgta ttgtatacct tcatgattcc
     3361 ttgtcgacac tccttacttg atacgactac ttacagcgtt tgttctttcc aactcctcaa
     3421 cccggttttc caactctatc accgtacgtt gcaggcattc aatcatcttg gccaccggat
     3481 ccggggtctg attatgagcc agatccacgc catgtcgaac ccggacccca tcctgaacca
     3541 ctattcggcc gggcaccccc accaccgttg tattgggcgg cacttcctgg agaactacgg
     3601 ctcctgcgcc gatcttggcg ttgtctccaa tcttcatcga acccagcacc gtggcgccgg
     3661 cagcaatcat cacattattg cctaccgtag gatggcgttt acccttctct ttaccggtgc
     3721 cgcctaaagt taccccctga aagagcgtaa catcatctcc gatctcggtg gtttctccga
     3781 tgaccacacc cgcaccgtga tcgataaaga atcgcctgcc gatccgggct cccggatgaa
     3841 tctcaatccc ggtaaagaaa cgggtgatct gggagataac ccgggccaaa agaaaccatc
     3901 gtttttgata aaaccaatgg cttagtctat acgccatcaa ggcgtggacc cccgggtaac
     3961 agagcagcac ttccagccgg gatttggctg ccggatcccg ttcgaagacg gtatggatat
     4021 cttccctgat acgttggaac atcccgctca cctttcctaa accgaggagt ccatactgaa
     4081 taaaaaaact tcgcctctgg tgacagagac gaagggatta cttcgtggtt ccactctgat
     4141 tgggaattaa tcgcgcacat tatgcgcgca ccccgctcat ctttgcgcta acgggcaaaa
     4201 accgggatgg actcaataac ggatcgtaac aggattattc cctgtccaat ccgattcgcc
     4261 caaccactcc ggggtgcaaa tggcctacaa cctgtaaggc ggttacaccg tacatcccgc
     4321 cctctctgaa tacaccgtcg taaacccgat ccccatcatc gttttaatct ttggttctat
     4381 tatattaact gctccgtctg aagtcaacaa caattccgtt atacccccac gtggtctatt
     4441 attccgcaaa cccgccatga tttaccgaaa cccggaacca atccgcccca caaaagaaac
     4501 cagctgctta atcagggact gaatatacgc aatcaaacgg gagaggaaag actgaggagc
     4561 cgtttgcggc tgcgtctcat tctgcctttg agtaaaattc cgtaactggg ttttgagtcg
     4621 cgtcaaatcc gggttcagac tcccgatctt gagcaacaag tccgccagtt cgctcttctc
     4681 ctccggggtg agtttgagat tttggtccct caccccctga tcgacgatgc ggagcgcctc
     4741 agtcttcgtg gtaatcctga ctgaaagcaa ccgttccttg caacgttcca ctaaaatcgc
     4801 ggcctgctcc ggtccgactt tttcgccgag atatccggta tcgatcaatt ccaaagctcc
     4861 aattcgtttg gtaccggagg gaatggaact cccggtcaat gtctcaaagg atttataaat
     4921 cccggttaag gcggcagtgc cggagaccgg ttgtggtgcc gtcgcgtaaa cacgggcatc
     4981 gctgattccg gcagtggcca aggtattggc aaagatatgg ggcgtgatat aagtaaggtt
     5041 tttcgtttca accttgatgc ccgctcccgg ctcaagcttt tccagataaa ccgaggagac
     5101 ggatttcgtt ccgatctgct cagccggaac caggccctcg aataatcgcc actcctcctc
     5161 attggtcacc ttaatacttt taatatcctt cgggccgatc ccctgcggca atgggaaatc
     5221 gcgaaaaacg atcacctctt cctccggggt gagatctgcg ccatatgtta ccacccgttg
     5281 gggctgcgtt gcggcactgc tgaccacaag aagcccgatt acgataccaa tcacccacca
     5341 gcgcttggtt gagatcatta ctattccgcc tctcgaaaac tatataaaac cggtttcgct
     5401 accgttatta tgacaaatcg agagcggcgg aattcgcaaa atatactaag gcaacagggg
     5461 tcgcgcccct acagggcgcg cggcaatagg caacgggaga ccggtgaggg gataaaattg
     5521 atagtcaaca agggctagaa atgaaaggcc ggggatccgg gaaaaaacca aaatgggcaa
     5581 cagggactag acaacaaggg ctgggaatgg aagacacagg atccagaaag aaactaaaaa
     5641 aggcaacagg gcttggcaaa aagatgttgc ctgctgcctg ctgcctgctg cctgctgcct
     5701 gtcgcctgtg cccgttgctt cacgccccac agggcactct actcgtgcct cgcgcctcat
     5761 gccttcaaca aataatgcct atccgaaagc tcccttagcc ttttggcact cgattccggg
     5821 gtgatatccc gctgcggttc ggccaataat tcatagccga ccatgaactt ccgtatggtc
     5881 gcgctgcgga gtagcggcgg ataaaagagc atatgccagg taaaaccggg atgttcccga
     5941 ccatcgcatg ggctttgatg cattccggcg gaatatggga atgaggtttc aaagagatta
     6001 tcatatttcg tggttaaaac ccggactgca tcggccaata gtctcaaatc cgccgggccg
     6061 aaatcggcca gtgaagccag ttgttttcgg ggaacgatca tcgtctcaaa cggccaaacc
     6121 gcccaaaacg gaactaaaac cacaaaaccg tcattgacgt agacaatccg ttcctcccgt
     6181 ttcagctcta cttcagcata gtcaaggagc atcggccggc cgtggctttg gatatactcc
     6241 gcctggctag cgcatttctt gactaactcg tccggaatac tttgagaagc ccagacttgg
     6301 ccgtgaggat gaggattgct gcaacccatc atcgctcctt tattctcaaa gatctgcaca
     6361 tacttgatat ccggaagggc gctgagttcc agatactggt cacgccacat ctccgccacc
     6421 ttcaacagat cttcggggct catcagtggc agggtcaaac ggtgggaggg tgaaaagatc
     6481 aataccttgc agataccccg gacactctcg gttttaaaaa agaaatcgtc gttgccgcct
     6541 gccgggacat cgggaagtag cgcggcgaag tcgttcacaa atgtaaaagt tcctgtatag
     6601 ttgggattct taacaccacc ggcccttacg tttccagggc agagataaca gccgggatca
     6661 tattccggtt tatcctcggg ggcaggtttt tcgacctggc cggtccaggg ccgcttggtc
     6721 cgatggggcg aaaccaatac ccaagccccg gtcaaaggat tacggcgacg gtggggatga
     6781 tcttcgcgat tgaattccat attctcgtct tcccttcttt caatattctt catatattat
     6841 ttcgatttct tgctttgatt ttccttttat ttcgagaaaa ataaaagccc acgagacagt
     6901 catttcccgt gggcaccttt agcgttttca gcctattcct tactgtgctt ccgatgccag
     6961 cgtttccgag aggccggcca attcctccga tgaagctgca atctgctggg ccatcgcagc
     7021 caacccgtta accacctcgg tcagattggt cattgtctgg gtagactctt tggaaaaaga
     7081 tgtggcctcg gtaatcttcg tgcctacttg gttgaccata gccgactggg agctcaggaa
     7141 ggcgccgact tcctttaaaa gcggaatact cgagttcaac tcgtcaacga ttgcagaaaa
     7201 cttttctgtc atggtattaa tcgactggat agtggtttcc agcacttccc cggcatttct
     7261 tgattctgtt tcagcgctgt cgatgcttcc agcgattttt ttcgtgatct cattcacatt
     7321 atccaatgct tgcttggtct cctctgccag tttacggaca cgtgtcgcta ccactgtaaa
     7381 accctttccg aactccccgg cccgggcagc ttcaatcgaa gcattcaacg aaagcaagtt
     7441 gatctctttg gaaatgctcg tcaaagtttg agatatctta gcgactgagt taatttcctg
     7501 aagcaagcca tcaaaaaccg cagtattctt ggaaaattga gagcggaaag aatggaccgc
     7561 tgttttaacc gcagttgaat cgtcaattcc gttcaatgca ctctgcgaga tggactcaac
     7621 cattgaaatc gttttctgtg tgagatcgct cttttctacg gttttttccg aaaaatcggc
     7681 caagtattcc tgggccgcct ccatctcatg ctggttttgt atcgcctcat ttgagaccgt
     7741 ggcaaaggac gcattcaact cctcggtctg ggcgctcatc tcctcggcac tggcggagag
     7801 ttgctggctg gatgtgctta agctctgact gctctgtccg atctcttgaa gcagcttctc
     7861 gatcttcacc agggattctt tattctggcg ctccttctgc cccaatctcc gcattaattg
     7921 gttggcgatc acggctgccg ccacgccggc cagggctaca aagagcatat acattcccct
     7981 tttaatcacg actgatgttg cccccgccac ttgagggtgg agttcgggaa caaagaacat
     8041 caacgaagca taggcaaata gcgatcccgt tccgtaaacc gcagtgacca aagggttaaa
     8101 gtataacacc gataagcaaa taccccagaa aaagttgaga aaagtgtcgg gattggcccg
     8161 ccccatcgtc aggttgacga tggcgatgac gatgatctcg caaaaggtca tcaaatactt
     8221 gatggcttta tgatacggat tcttaaacat aagatagatc ggataaccga gaaatacaag
     8281 gccgctcccg gtcacataga ccaataccat gtacgagaag gtattatcat gcatcaataa
     8341 tttggcgacg agagcaaaga gatccccgat taacagaata gcggagctca caatgatatg
     8401 aatcttgttc accctgttct cattttccgc ataaagcatc aactcggcgt tttctaggtt
     8461 ttccacggga atgccccctg ttacttaatg aacttctcaa tacgccgggc taccgctttt
     8521 ggtatggcta ccggcaaaaa atgtccgtaa cgcgggaaaa tttccttctc cgctccggag
     8581 attcggttac gacagaattc ggcgtctaca ggtggagcta tcggatcctt ctccccggtt
     8641 ataatcaacg ccggtagctg gatctggtca atctcctgcc gccggtcgaa ccggagtaca
     8701 caattttgga aatcggccaa cattacgggc ttatgggtca acgagtaaaa aagactggtc
     8761 accacgatac ggaaaacatt ccagttccag atcccgccgt aacttaaaaa atgaaactca
     8821 tcaaattcat ctctccggat ctgttctttg aaggagtctt cgagattctt ctccatctga
     8881 ggtgttgtgt cgaccatgac caaacctttc aaaggggtct tcttccttat ggccagctca
     8941 attgcgatga acccgcccat cgagtatccg acgagatata tgtcctttaa ttttaaggtc
     9001 cggacaaact cctcgatgaa gtcggcgtat tcggcaactg atcccagcgg ccggccggcg
     9061 cttttaccat gccccggcag atctatgagt acccggttat actttgaaag tttagttgcc
     9121 aacggtttta aaatattcaa ccgtgttccg gcgccatgca tcaataataa agtcggggcg
     9181 ccatctttgg gcagaacctg ataatgaatt ttcacatcgt tgacggtaca gtacataaaa
     9241 tagtcctcct gttattgata tgtaaattgg tacaatgatt caagctgggg ttttaaatcc
     9301 tccataaaat atttcgacat gaaatgaaaa aagtttatct atttttattt gggtgacctc
     9361 ttaaataaaa attataaaac taacaatttg cgaatatagg ggttattcac atttgtatca
     9421 tactcatacc gggaaaataa taacgccgca ggtttcatcc tacggcgttt caaaacaaac
     9481 ggttgtctat gtgaagcgaa gtcgagacgg gtgtttccaa tgaaaaaccc gttacctgcg
     9541 gttcaattcc tcccataaat ccggggtttc ggcttgaatg aactcaacca gctcgtgatc
     9601 gccgatcacg gtatttctgc cgttttggta ttgttcatcg acccagccct tgatcttcag
     9661 caggcgtgaa tcacgctttt ccagcttccg gtcatccctc agattaaagt aactgttaac
     9721 ccaatgggcc aacccggccg caccggaatg ttcgttcacc gctactacca ctttccggtt
     9781 aagcagtttt tcggtgttga aaatattata aatctcctca tctttcagga gtccgtcggc
     9841 atgaatcccg gaacgggtga agttaaattc tcttcctacg agtggtgtca tgggcggaat
     9901 ctgatagccg atctccttgt tgtagtaatc ggcaatctcg gtgattaccg tggtatccat
     9961 accatccaat gacccgcgga gcgaggcgta ctcgaagatc attgcctcta aaggacagtt
    10021 accggtccgc tcgccaatgc cgagcaaagt gcagttcact ccggaacaac cgtaaagcca
    10081 cgcagttccg gcgttagata cagccttgta aaaatcatta tgtccgtgcc attccagttg
    10141 atcgctggga acgccggcat gatgccaaag gccgtatata attccgggca cgctgcgcgg
    10201 caaggcaacc ccgggatagt ttaccccaaa tcccagcgtg tcgcaggccc gaatcttgat
    10261 cttgatcttc gactcttcct ggagtttcat caattccagt acaaatggaa ccacaaaacc
    10321 ataaaaatca gcccgggtga tatcctccaa atggaccctg ggggtcaccc cgatctccag
    10381 cgcgctctta acaatgctta ggaactgctc cattgcttgt ttccgggtta gtttcaactt
    10441 tttaaagata tgatagtcgg agcaactggc gagaattcca gtctctttta gccccatctc
    10501 cttgaccagc aggaagtctt ccttcaccgc cctgatccaa ctggtgatct ccggataatc
    10561 gtaacccagt tccatacact gtgtggcagc ttccttgtcc ttattgctat ataaaaagaa
    10621 ctccgtctga cggatgaccc ctttgggtcc gcccagccgg tgcaggtatt tataaagatc
    10681 cacaatttgt ttgacggtat acggttctct ggcttgctgc ccatcacgga aggttgagtc
    10741 ggtgatccag atcttctccg gaggccgcat tggaacatga cggtgattga atggaacctt
    10801 gggcacctcc tcgtaattga aaaggtaccg ataaagatgg ggttcagtca catcttgcaa
    10861 tgcgtaaata tactccgact gttccaaagt attgctgtgt ttgttaaatt ccagcattca
    10921 ctatccacca cccatataca actactgcac cttaccctcg aaatggcgcg gtaaatcgtt
    10981 gcttttatta aattgtccat aatattcaac attgaatcgg gctttcctct attaaaaatg
    11041 aatgaatccc gtatacatat aaaatgaaaa ttatttccgg attgtggaat ccattaccaa
    11101 tcattatttt ttactatagg caaaaagaga cccatctttc acttgaataa ttatcatagt
    11161 atgataggaa aaatcaaaaa aacgcccgaa ggcgtttttg gtcttttatc gatcaagttt
    11221 ctatttgata ttggtcaata tcaacccgag tacgcaaaag gctaatccca aaacattcag
    11281 cccgctcaag tgttcccgga acagaagtac gccgatgggg atgagcaaca gagccaccgc
    11341 cacattggaa aagacccccg ccagactgat ttgccaaccc atccggtagg ccaggagata
    11401 ccccaactca agaccgatga tggccactcc caaaacgata ctggcccaat tcaaggattt
    11461 aatcgattcc ccgatccccg gaccttgccg gtagaaaggc agtatcgcca aggatatcaa
    11521 gatcgctgta agatacgttg cgatcagcga aacaaacggg ttaacatttc caga
//
LOCUS       DASVMB010000318         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_406514,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000318 DASVMB010000000
VERSION     DASVMB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406514"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VF531_09090"
     CDS             <1..814
                     /locus_tag="VF531_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7714166.1"
                     /translation="LVQKPPLFEPGEPQFWDDPYISQSMLKAHLDPGHDAASRKPETI
                     DKTVHQFLSSGIVKPGSRVLDLGCGPGLYTERLYRVGIEVVGLDISKRSLDYAVQHAN
                     EAGLVIEYVRMNFFDMDYREEFDAVLQVYGELCVFSDDKRDILLRKIHQSLKKGGRFI
                     FDVSTRVLRRKYGLKNHWYICEGGFWRPGRHIVLEEGFDYSEADTWLDQYIVIDDQAC
                     KVYRNWFHDYSLESISRVLHSVGFEVKQAWNDLTGSPFLEGGEWIGIVAEKK"
     gene            1001..1813
                     /locus_tag="VF531_09095"
     CDS             1001..1813
                     /locus_tag="VF531_09095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714167.1"
                     /translation="MHEITSYEFSKVLPLFSGWDHHMAIRAAAEGKARSRVWVDFDDS
                     PTAAAVCFGVRLLIAGDSQNIACRDAIVDFVQNAVLQNQIERMRHFVMIFWDEPGWKE
                     VLPDSLEGYNPILREREYYRQDMANIFNNPALPAGYELRRVDSSFLNEREWRHKSALQ
                     YEMCSERVSLEDFLEHSFGVCAVQGDEIAGWCLSEYNNSSGCEVGIELLESHQRKGIG
                     TALTLALFSEARKRGLPYVGWSCFKDNLPSRATARKAGLSKVKDYQVIVVQR"
     gene            1843..1968
                     /locus_tag="VF531_09100"
     CDS             1843..1968
                     /locus_tag="VF531_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714168.1"
                     /translation="MKNDELFIFAPNHCSVDFQISTRYNQIIGAGHSCKGGVFGD"
     gene            1961..2203
                     /locus_tag="VF531_09105"
     CDS             1961..2203
                     /locus_tag="VF531_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7714169.1"
                     /translation="MIDNIKIGQRIAMLRKEKGFTQEEIADELGVTGQAVSKWENGNA
                     LPDLLLLPQLAGMFEISIDRLLSPNDLYIFPSNMGL"
     gene            2429..2617
                     /locus_tag="VF531_09110"
     CDS             2429..2617
                     /locus_tag="VF531_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714170.1"
                     /translation="MEGIKHQVIRLIEALPEGATIEDIMAELYFKLQVDRGLKELDEK
                     KGIPQEDVETRFSKWLSE"
BASE COUNT          853 a          588 c          858 g          896 t
ORIGIN      
        1 actcgttcaa aaaccaccgc tttttgaacc gggagagccg cagttttggg atgatcccta
       61 tatctctcaa agcatgctta aggcacatct tgacccgggt cacgatgcgg ccagccggaa
      121 accggagacc attgataaaa ccgttcatca gtttttatcg tccggtatcg tgaaacctgg
      181 atccagagta cttgatctgg gatgcgggcc ggggctttat acggaacggc tataccgggt
      241 tggcatagaa gttgtcggct tggatatttc caaacggtcg cttgattatg cggtacagca
      301 tgctaacgag gcggggcttg taatcgagta cgtccggatg aatttctttg atatggatta
      361 ccgggaagag tttgacgccg tactgcaggt gtacggggag ctatgtgtat tctccgatga
      421 caaacgggat atcctgctgc ggaagattca tcaatcgctg aaaaagggcg gccggtttat
      481 ctttgatgtc tccacccggg ttctgcgaag aaaatatggc cttaaaaatc actggtatat
      541 ctgtgaaggc gggttttggc ggccgggaag acatatcgtg cttgaagagg gttttgatta
      601 ctcggaagca gatacttggc tggatcagta tattgtcatt gatgatcaag cctgtaaagt
      661 gtaccgaaat tggtttcatg attattcgtt ggagtctatc agccgggtgt tacactccgt
      721 cgggtttgaa gtgaaacaag cgtggaacga tttgaccgga agcccatttc ttgagggtgg
      781 cgaatggatc gggattgttg ctgaaaaaaa ataacataag gtatacgaaa gacacggtat
      841 ccatcgtatg ttttatcgtt tccccggaat accgcagaaa gatcatggcc ccatctcgct
      901 atacataaaa aataattttc aaattgtgaa tgaatttgag gactatttag tcaagcggtt
      961 ggaagttgat aaagattaaa taggtggtgg gaaatgaaca atgcatgaga ttactagtta
     1021 tgagttttca aaagttttac cccttttttc cggttgggat catcacatgg cgatacgggc
     1081 cgcagccgaa gggaaagcga ggtcccgggt atgggttgac tttgacgatt cgccaacagc
     1141 tgcagccgta tgttttggcg ttcgcctgct tatcgccggt gattcacaga atatagcctg
     1201 ccgcgacgct atcgttgatt ttgtacagaa tgctgtttta caaaatcaaa tcgaaagaat
     1261 gcggcacttt gtgatgatat tttgggatga accgggctgg aaggaggtac tgccggattc
     1321 gctagagggc tataacccca tcttacggga gagggagtat tacagacagg atatggcgaa
     1381 catcttcaat aaccctgcat tgccggcagg ttacgaatta cgccgggtcg acagttcttt
     1441 tttgaatgaa cgggaatggc ggcataaaag cgctttacag tatgagatgt gttccgaacg
     1501 ggtaagcctg gaagattttt tggagcatag ctttggcgta tgtgccgttc agggcgatga
     1561 aattgccggc tggtgccttt ccgaatacaa caattccagt ggttgcgagg ttgggattga
     1621 actattggaa agccatcagc ggaaaggaat cggcactgcg ctcaccctgg cgctgttctc
     1681 tgaagcccgt aaacggggat tgccatatgt cggctggagt tgttttaagg ataatctgcc
     1741 ttcccgggcc acggcaagga aagccggatt gagtaaagtt aaagattatc aggtaattgt
     1801 ggtgcaaaga taaaagcttc tgttttatct ggagggaaat cagtgaaaaa tgatgagttg
     1861 tttatctttg cccccaacca ttgttcggtg gacttccaga tttcgacaag atataatcag
     1921 attatcggcg ccgggcattc atgcaaggga ggggtttttg gtgattgata atattaaaat
     1981 cgggcagcgg atagcgatgt tacgcaagga aaagggtttt acgcaggaag aaattgccga
     2041 tgaacttgga gtgaccgggc aggcggtttc aaagtgggaa aacggcaatg cgcttccgga
     2101 tctgttgctt ttgccacagc ttgccggcat gtttgaaata tccatcgacc ggttgctttc
     2161 gccgaatgat ttgtacattt tcccatccaa catgggatta tagttccgtc gatgggttgg
     2221 ttgcctacga ttacgccacc cccggtttaa aagcttttgg ttttgagcgc aagataaaag
     2281 acaggatccc taaagagcaa cgtaaagaag taagggatga aattattgaa agtattaata
     2341 aaggacgtcc ggtgcgggtg agtagggcta attatcgagg tgttgtacac cggattttca
     2401 atggtataat aaagaggggg tgatagtttt ggagggaatt aaacatcaag ttattcgcct
     2461 gattgaagct cttccggaag gagctacgat tgaagatatt atggctgaac tctattttaa
     2521 gttacaagtc gatcgtggtt taaaggaatt ggatgaaaag aaggggatac ctcaggaaga
     2581 tgttgaaact aggttctcga aatggctatc cgaataaggt ggtcgcctag ggctgtaaaa
     2641 aatttggagg atatttgcat atatattgaa agagattcat ttcattatgc agcggatttt
     2701 gcgaaaagag tttttgctct tattcaagat ctaaagcaat ttcctttatc cggccgggtt
     2761 gtacccgagt ataatgatgc ataagcttgt cgtgcttttt gcacggcctt tttattatag
     2821 agttaagtaa ggtgactgca gagaacctcc gggaaatttt aattatagct atttggtagc
     2881 ttgagatggt tatttgtcgg atctatgatg gaagtgtaca cgatatcatg tacatagcgg
     2941 tatccggaac cgactttcat aaagaaagga gtggtcttga ggtgaagatt acgatggagg
     3001 tggccatcgt ttgatggccg gggggtgtgc cggaccgaaa aggtttggcc gccccttttc
     3061 gttttaactg acaaacgacc ctgaccaaaa ttatggaaat catcgcgtta aactctaagg
     3121 ctttaagtca atagcccgcg ctattgactt aaagcctttt tgttaccttt cgattatcat
     3181 tatctttcga ttatc
//
LOCUS       DASVMB010000319         8741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_332911,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000319 DASVMB010000000
VERSION     DASVMB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332911"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VF531_09115"
     CDS             complement(<1..817)
                     /locus_tag="VF531_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7714171.1"
                     /translation="METAPIHEEPQVEPMSYGIFPDFDPLIELTNDFTEFLLLMHRKR
                     WDSWDAFVLNQRAEALGLSQGFEELLVLTHLAEHWRQNGVIPYPHQIETVRKVISQMR
                     GRAILADEVGLGKTIEAGMILKEYLLRGLVKRFLILTPAALCRQWEAELREKFAIPVL
                     IARREYDWNHYDFLLASMDTAKKESHRTEILKGYFDMIIVDEAHKLKSTVTQNFQFVQ
                     GIQKKFFLLLTATPLQNDLKELFNLITLLRPGQLGNYRQFKQKFTKDKRTPQNA"
     gene            1019..3481
                     /locus_tag="VF531_09120"
     CDS             1019..3481
                     /locus_tag="VF531_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7714172.1"
                     /translation="MDVNRFLQELTASRDYQDQIITIRELPIREAVYGTPDPPLPAEL
                     SGLLKHLGIERLYSHQAEAINEIRAGHHTVIVTGTASGKSLCFLIPVLERYLANPQAR
                     ALFLYPTKALAQDQLKNIARLIEPLAGPKPLAGTYDGDTTTNARKTLRDKANLILTNP
                     EMLHQGILPKHPGWSEFLGNLQYVILDEIHTYRGVFGSNVANVIRRLRRICRHYGSDP
                     VFIGTSATVNNPLEHAEKLLGVKMSLVDQDGAPKGKKYFVFWNPPYLDATKSERRSAN
                     SEAARLMVELIRERIPTITFTKARVVTELIYRYVQDQLQTVSPSLAKKVRAYRGGYLP
                     EERREIERQLFSGELLGVTSTNALELGIDIGQLEACLIVGYPGTIASTWQQAGRAGRS
                     KDESLVIYIPHQNPLDQYLAKHPEYFFEQNPEAVVVDPDNPHIVMGHIRSAAFESPVT
                     AYDCQSFGTYSPAILELLGEAGELRRSADSWYWQSPGFPATEVSLRNIADNTFSIVDI
                     SVEPHKVIGTLDEASAYEQIYTEAIYLHDGETYFVEEMNVPQKVSYVRKIDVDYYTQS
                     VTEVQLIIKGRELEGKWRKGDTGFGDVEKMEKPYLFRKIKFGSRDSIGFGKISLEPVT
                     METKAFWVIPPASTLRLVKDFGRDPVESLLGIANVFTEVLPLLVMCDGGDIGSMVDLA
                     NNGAPSLFIYDRYPGGLGFAHRAFERVEDLFTAARDLIEACSCEGGCPSCVGSPLPPS
                     PQLDPDTTGKGRIPDKEGSLVILHDLLELPPYIPRVAKEPVQATPTKADPVVAYPPFV
                     PLPEKVEEQLRQKLAGLKRKKS"
     gene            3756..4796
                     /gene="mtnA"
                     /locus_tag="VF531_09125"
     CDS             3756..4796
                     /gene="mtnA"
                     /locus_tag="VF531_09125"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7714173.1"
                     /translation="MSWKTLDWDQKHLILLDQTLLPGEVRYLNLSSYLEVAEAIKRLQ
                     VRGAPAIGIAAAYGVVLGAREFETNPRFHERVIQVIQTLAQTRPTAVNLFWALDRQRR
                     VFEANRERPVPEIVRLLETEAGRIASDDATINRKIGEYGASLFQDGMGILTHCNAGAL
                     ATFAYGTALAAIRVAVEQGKHLEVFADETRPLLQGARLTVWELMQEGIPVTLITDNMA
                     AAVMRLGKVQAVIVGADRIASNGDVANKIGTYGVAILAKEHGIPFYVAAPLSTIDFKL
                     ESGAEIPIEERAIDEVTTIGGVRVAPPGARVFNPAFDMTPARYITAIISERGIARAVG
                     GDFRESLRSWRD"
     gene            5109..5282
                     /locus_tag="VF531_09130"
     CDS             5109..5282
                     /locus_tag="VF531_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714174.1"
                     /translation="MAKENQPGSAEKTQAKKSDIEAAEELTEVSGQETAEDLPYRSPE
                     KGMNKRQQRMKKQ"
     gene            6171..6692
                     /locus_tag="VF531_09135"
     CDS             6171..6692
                     /locus_tag="VF531_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714175.1"
                     /translation="MYQWRVTKYNPIFRDERGAYKKDEWTSISDVGKTYTGKDFTLDE
                     YLKVEDLYVKAISEFMDSMKISALSVINLGKPERLMFNSRFESIYNHDMVKLYTNIQD
                     DKRIGIEEVMIISRLVLRENLCCKLREKHKMFVHFGYDYYMYLGCSSKNQSVIQKIED
                     NGLFVEEYISPYR"
     gene            6790..7383
                     /locus_tag="VF531_09140"
     CDS             6790..7383
                     /locus_tag="VF531_09140"
                     /inference="COORDINATES: protein motif:HMM:NF037477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HEX7714176.1"
                     /translation="MREFDRTKTLEELEEDIWGEPEYDSHLVTECHRLRKKRVCDFDI
                     ENLRIMIGQEIGMDYLVPIALEFLKENPLVQGDFYPGDLLNNVLKINFSFWEENHDLK
                     ETLDLIIQDTPLVPEIEESLDSYLKVHKAIHECSEMMIGDNFKFLIYKIIDNWYLSSI
                     TYADGKAVSDGEAERIGEIMNEYSFLIKFCPFCGMKL"
     gene            7365..8132
                     /locus_tag="VF531_09145"
     CDS             7365..8132
                     /locus_tag="VF531_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7714177.1"
                     /translation="MRHEIIIFGSIFTDTMAEMTWEEVKKAAEYGSVVLLPIAVVEEH
                     GPHLDLSIDIYLTYLICRFIKHELKEKGIESVVAPPYYWGINECTKNFPGSFRIKPET
                     FKAILHDIIESLQSWGFRKIFCISVHGDPTHNNIMDSSIQEIKENLSVDVYSINVNTL
                     FAELNPFSLLSRPGKFAPDYHAGADETAWIQAFYPDKVNVEIAKKLKPQAGFEPLGYF
                     GDPAGFELEPNAKEAFLTIAKRWSALIESYLKNEQRG"
     gene            8213..8722
                     /locus_tag="VF531_09150"
     CDS             8213..8722
                     /locus_tag="VF531_09150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7714178.1"
                     /translation="MAQIFNQKNLSFEPDESPIPEFSWHTSQNLAEIVKSKHLQFDVR
                     LLDPDKYSCPYHFHRNSEEIFVILSGKVTLRTPDEFVELSEGDIVFFETGPQGAHQLY
                     NHTQQACKYLDIRTEAGIDICEYPDSGKINIRPYGELYQSEDKVDYYKGEDNVREKWM
                     NLSAKFVRK"
BASE COUNT         2327 a         1979 c         2254 g         2181 t
ORIGIN      
        1 tggcattttg cggggtccgt ttatccttgg tgaacttctg cttgaactgg cggtaattgc
       61 cgagttgacc cgggcggagc agggtgatca ggttgaagag ctctttcagg tcattttgga
      121 gcggtgtagc agtcaaaagc agaaagaact tcttttggat cccttgaacg aactggaagt
      181 tctgggtgac tgtggacttg agtttatggg cctcatccac aatgatcatg tcgaaatagc
      241 cctttagaat ctcggtacgg tgcgattcct tcttggccgt atccatcgag gccagcagaa
      301 agtcataatg gttccagtcg tactcccgcc gcgcaatcaa taccggaatg gcgaacttct
      361 cccgcaattc ggcctcccat tggcggcaaa gggcggcggg agtgaggatc aaaaaccgtt
      421 tcaccagacc ccggagcagg tattccttga gaatcatccc tgcttcgatg gtttttccca
      481 accccacttc atcggcaaga atcgccctgc cgcgcatctg ggagatgact ttccggacgg
      541 tttcgatctg gtgcggatag gggatgactc cgttttggcg ccagtgttcg gcaagatggg
      601 tcagtaccag caattcctcg aatccctggc ttaaaccgag cgcttcggcc cgctggttca
      661 aaacgaaagc atcccaggaa tcccatcgtt tcctgtgcat gagcagcaag aattccgtaa
      721 aatcattcgt caactcgatc agcggatcaa aatcggggaa gatcccgtat gacatcggct
      781 cgacctgggg ttcttcatga atgggcgcgg tctccaccaa ttaatcctcc tctgcggtgc
      841 aggtagaata gtaatgactt gttagcttca ccggagttat agcttatccg ctaaccggat
      901 ggtttaacct taggattggg aagggaggaa cgcgaaaagc gtgtccgggg caggaaggaa
      961 ttttagtttt gtcgaattaa ccaagagtaa atatgaggga tttacgaggt aaactgcgat
     1021 ggatgtcaac cgctttttgc aagaactcac cgcttcccgg gattaccagg atcagatcat
     1081 caccattcgc gaactgccta tccgggaagc tgtatatgga acccccgatc cgccgctacc
     1141 tgcggagctc tccggtctcc tgaagcattt ggggattgag aggttatact cccatcaggc
     1201 cgaggcgatt aacgaaattc gcgccggaca ccatacggtg atcgtcaccg gtactgccag
     1261 cggcaaatcg ctttgtttct tgatcccggt tttggagcgc tatttggcca acccgcaggc
     1321 gcgggcgcta ttcctgtacc cgacgaaggc cttggcccaa gatcagttga agaatatcgc
     1381 ccggctgatt gaacctctgg cagggccgaa acctttggcc gggacctatg acggcgatac
     1441 caccaccaat gcccggaaaa cactccggga taaggcgaat ctgatcctga ccaacccgga
     1501 aatgctccac cagggaatct tacccaaaca tcccggttgg tccgagtttc tgggtaacct
     1561 gcagtatgta atcctggacg agatccatac ctaccgcggg gtattcggtt ccaacgtggc
     1621 caacgtcatc cgcaggctgc gccggatctg ccgccattac ggcagcgacc cggtattcat
     1681 cggaacctcc gccacggtca acaatccttt ggaacacgcc gagaagctgc tgggagtgaa
     1741 gatgagtctg gtcgatcagg atggcgcgcc caaggggaaa aagtatttcg ttttttggaa
     1801 tccgccgtat ctggatgcga ccaagtccga acgccgcagc gccaactcgg aggcggcccg
     1861 gctcatggtg gagctaatcc gggaacggat acccaccatc acctttacca aggcacgggt
     1921 ggtcaccgaa ctcatctacc gttatgtgca ggaccagctc caaaccgtca gccccagtct
     1981 ggccaagaaa gtccgggcat accgcggcgg ctatcttccc gaagagcgcc gggagatcga
     2041 gcggcaactt ttttccggag aacttttggg tgtaaccagc accaatgccc tggagttggg
     2101 gatcgatatc gggcaattgg aggcttgttt gatcgttggt tatccgggaa ccatcgcttc
     2161 cacctggcag caggccggcc gggccggccg ttccaaggat gagtcgctgg tgatctatat
     2221 tccgcatcag aacccgcttg atcaatatct ggccaagcat ccggagtatt ttttcgaaca
     2281 aaacccggaa gcggtggtgg ttgatccgga caacccgcat atcgtcatgg gtcacatccg
     2341 tagcgccgcc tttgagtccc cggtgaccgc ctacgattgc cagagtttcg gaacttattc
     2401 cccggccatc ctggaactcc tgggtgaggc gggtgaactt agacgtagcg ccgacagctg
     2461 gtactggcag agtcccggtt tcccggccac cgaggtcagc ctgaggaata ttgccgacaa
     2521 caccttcagt atcgtggata tctccgtcga accccataaa gtcatcggga ccctggacga
     2581 ggccagcgct tacgaacaaa tctacactga ggcaatttac ctccacgacg gcgagaccta
     2641 cttcgtggag gaaatgaacg tgccccagaa ggtctcctat gtccggaaga ttgatgtcga
     2701 ttattacacc cagagcgtaa ccgaggttca gttgatcatc aaggggcggg agcttgaggg
     2761 taagtggcgc aagggggata ccggattcgg ggatgtggag aagatggaga agccctatct
     2821 tttccgtaag attaagttcg gatccaggga cagcatcggt ttcggcaaaa ttagcctgga
     2881 accggtgacc atggagacca aggctttttg ggtgattccc cccgccagta ccctgcggtt
     2941 ggtcaaggac ttcgggaggg atccggtgga gagcttgctg ggaatagcca atgtcttcac
     3001 ggaggttctg ccgctactgg tgatgtgcga cggcggcgat atcggctcca tggtggattt
     3061 ggccaataac ggcgctccat cgctcttcat ttatgaccgt taccccgggg gcttgggatt
     3121 tgcccatcgg gctttcgaac gcgtggagga cctcttcact gccgcccggg atttgattga
     3181 ggcctgttcc tgcgagggcg gttgtccttc ctgcgtgggt tcgccgttgc cccccagtcc
     3241 ccagttggat ccggatacaa ccggcaaagg ccggattccc gataaagagg ggtccctggt
     3301 gatcctccac gacttgttgg aactcccgcc ctatataccg agggtcgcca aagagccggt
     3361 acaagccact cccaccaagg cggatccggt ggtggcctat ccgccgtttg tgccgctgcc
     3421 cgagaaagtc gaggagcagc tccggcagaa actggccgga cttaaacgga aaaaatccta
     3481 actattattt gcgaacgtgt tcatctgaca ggaatcggga aagctagcta ccctcacccc
     3541 tcagccaaca tccgggcctc gcgtacacgg tacgcgcgcc ctcccccata acgctatcaa
     3601 aaagcgataa ctatgggaga gggccgctga cttgaatcgg tctgatgggt gagggcattg
     3661 gggccggagc cgacagttgt ttttgacttt tgacttttaa cttttaactt tcgactttgg
     3721 actttagact ttcgactact gctggagtga tgctgatgtc ttggaaaaca ttggattggg
     3781 atcaaaagca tttaatttta ctcgatcaaa cgctgctccc gggagaagtt cgatatttga
     3841 acttaagcag ttatctggag gtggcggagg ccatcaaacg cctccaagtg cggggggcgc
     3901 cggccatcgg gatcgccgcc gcctatggag tggttctggg cgctcgtgaa tttgaaacga
     3961 atccccgatt ccacgagcgg gtgattcagg tcattcaaac cctggcccaa acccgcccca
     4021 cggcggtgaa tcttttttgg gccctcgatc gccaacgccg ggtattcgaa gcgaaccggg
     4081 agcggccggt acccgaaatc gtccggctgc ttgagacgga agcggggcgg atcgcctccg
     4141 acgatgctac gattaaccgc aagatcgggg aatatggcgc ttcactcttt caggatggga
     4201 tgggaatttt gacccactgt aacgccgggg cactggcgac attcgcctac ggcacggctc
     4261 tggcggcgat ccgcgtcgcc gtggaacagg gaaaacatct cgaggtattt gccgatgaga
     4321 cccggcctct acttcagggg gcgcggttaa cggtctggga attgatgcag gaggggatac
     4381 cggtgaccct gatcacggac aatatggccg ctgcagtgat gcgtctcggc aaggttcaag
     4441 cggtgatcgt cggggcggac cggattgcgt ccaacgggga tgtagccaat aagatcggca
     4501 cctacggtgt ggcgattctc gccaaggagc acgggatccc gttttatgtg gcggcgccgc
     4561 tttccacaat cgatttcaag ctggagtcgg gagcggagat tcccatcgag gaacgggcaa
     4621 tcgacgaagt gaccacgatc ggaggagtac gggtggcgcc tcccggagcc cgggtcttta
     4681 acccggcctt cgatatgacg ccggcccgtt atattaccgc cattatctcg gagcgaggga
     4741 tcgcccgggc ggtgggtgga gattttcgag agtctttacg cagctggaga gattaacgcg
     4801 cgtacgcgcg tatccgcgtc atccgtaaag cggcaatttg gcatataccc ggtaacagtg
     4861 cggacagaac catttgccat cggcccgcaa aagcttgtga ttgttgcaga taggacaggc
     4921 cattactctc acctcagttt cattgtatga ttttggggac tgaaaagata ggccgtaaca
     4981 ttggtccgga tgttaaaatt ttgatggcaa attccggtct ggatggttta accatcgcct
     5041 cgccgagtga tactaatcca gggtataggg aaaggcctat actacaaaag gaaaaggaga
     5101 ggttgatttt ggcgaaagag aatcaaccgg ggtctgccga aaaaacccag gcgaaaaagt
     5161 cggacattga agcggcggag gagttgacgg aagtcagcgg ccaggaaact gccgaagatc
     5221 tcccataccg ttctccggaa aaagggatga acaaacggca acaacggatg aaaaaacaat
     5281 aactccagcg acgaggggtt aatcgagccg tttggaccaa tttgactctg atagggttgc
     5341 agctgcgacc ctatttttta tcgccgttca ctatatatat tcggtaatta tccaggtcat
     5401 cttgaattct tgaagattcg gcaaagcttt ggattacgcc tattcgcggt aattggatga
     5461 aatatgatat gagttgaaat aaaatatagg ttttgttttc agaaacggtt gatctatgta
     5521 tcaaagacaa gtgcagcatg tcgatgctgc cggggagcgg ggtgtccccg catactttca
     5581 cagatttccc ctcctggagg tcgcgcttca ctaagcgcgc agggataggg gtggttggcc
     5641 atgaataatc aaaaggtttg taggacaaca attggtgagt gtaacgtaaa ttaaacaatt
     5701 caaattgtaa agctattact ccttcaccaa attgatgact aaggccagca tatgctggct
     5761 tttgtcttat tattcaaagt gtgaagaatt tgagggaaaa gcattccgag tttattctaa
     5821 atcgaaattt ttagattacg ttaaggcaac tacttatgta actactgatt atccaggtcc
     5881 atacaaccat tatggtttta tatgcctaga tcacattatt aatgttgttt cgagcgatgc
     5941 accaattgtt acggaatata attgtaagta accgttatcc atggatggta tataactgta
     6001 ttcaagttag cgaggagagg taagtcaaag taaaccccgc aaaattaaga taggagctat
     6061 ctaaccttta ggattttgat aacgcagaga cgataaataa aacacctgat tcttctgagc
     6121 tatccggtac tgttaagttt catctgcaac ccccaaaaaa ggagaaacca atgtatcaat
     6181 ggagggtaac aaaatataat ccaatattta gggatgaaag aggggcatac aaaaaagatg
     6241 aatggacctc aatttctgat gttggaaaaa cctatactgg gaaagatttt acacttgatg
     6301 aatatttgaa agttgaggat ttgtatgtta aagccatttc agaatttatg gactccatga
     6361 aaatatcagc tttatcggta ataaacttag gtaaaccaga aagacttatg ttcaactctc
     6421 gtttcgaaag catatataat cacgatatgg taaagctcta caccaatatt caagatgata
     6481 agagaatagg gatagaagaa gtgatgataa ttagtcgttt ggttttgcgg gaaaacttgt
     6541 gttgtaagtt gcgggaaaaa cataagatgt ttgttcattt tgggtatgat tattatatgt
     6601 atttaggctg ttcatccaaa aatcaaagtg taattcaaaa aattgaggac aatggtttat
     6661 tcgttgagga gtatatttct ccatatcgat aaaaataggg tttgaaagtg gctctcatcg
     6721 tagcagccct tttgcaacat tagacgatat ccgaaaaatc aggccaagcc gcgtcctttg
     6781 gggtgaatca tgagagaatt tgatcgaaca aagactttgg aagaacttga ggaagatatt
     6841 tggggagaac cagaatacga ttctcacctt gtaaccgaat gtcatcgctt acggaaaaaa
     6901 cgtgtttgtg attttgatat tgaaaattta cgaatcatga ttggccaaga gatcggtatg
     6961 gattacttgg ttcctattgc tttggaattt ctcaaagaaa atccactggt acaaggtgat
     7021 ttttatccgg gagatttatt aaacaatgta cttaaaatta acttttcatt ttgggaagag
     7081 aaccatgact taaaggaaac attggattta attattcaag atactccgct ggttccggag
     7141 attgaagaat cattggattc ctatctgaaa gtccataaag cgattcatga gtgttcagag
     7201 atgatgattg gcgacaattt taaatttttg atttataaaa ttatcgataa ctggtatttg
     7261 tcttccatta cctatgcaga tgggaaagca gttagtgatg gggaagcgga aaggattgga
     7321 gaaataatga atgaatattc ctttctcatc aagttttgtc cattttgcgg catgaaatta
     7381 taatcttcgg gtcgatattt accgatacca tggccgaaat gacctgggaa gaggtcaaaa
     7441 aggctgcgga atatggaagc gttgttttac tacccatcgc tgtcgttgaa gagcatggcc
     7501 cgcatctgga tttaagcatc gatatttatt taacctatct tatttgcaga tttatcaagc
     7561 atgaactcaa agaaaaggga atcgaatcag ttgttgcccc gccgtattac tggggtatca
     7621 atgaatgtac caaaaatttc ccggggtctt ttcggataaa gcccgagaca tttaaagcga
     7681 tattgcacga tatcattgaa agccttcagt cttgggggtt tagaaagata ttttgcatca
     7741 gtgtacatgg agatccaacc cataataata tcatggattc gtcaattcaa gaaattaaag
     7801 aaaatctatc ggtagatgtt tattctatca atgtaaatac actatttgca gaactgaatc
     7861 ccttttccct attatcaagg cccggcaaat tcgcccccga ttatcatgcc ggagcggatg
     7921 aaacagcatg gatacaggct ttttaccctg ataaggtcaa tgtggaaatc gccaaaaaac
     7981 tgaaacctca agccggtttt gagcctttgg gctatttcgg cgatccggcc ggctttgaac
     8041 ttgaaccaaa cgccaaagaa gcatttttaa cgatagcgaa gcgatggtcc gcattaattg
     8101 aatcatattt aaagaatgag caacggggct aaaaagattc actgataagt acgagatata
     8161 aataccttga atgaacttca aatcgacgaa tctctaaaag agggggattt aaatggcgca
     8221 aatattcaat caaaagaatc tttcatttga accggatgaa tcaccgattc cggagttttc
     8281 ctggcatacg agtcaaaatc tagcagaaat cgttaaatca aaacatttac agtttgacgt
     8341 acgattgctt gatcctgaca aatattcctg cccctatcat tttcatcgaa attctgaaga
     8401 aatattcgta atcttatctg gaaaagtaac ccttcgcacg ccggatgagt tcgtcgaatt
     8461 aagtgaaggt gatattgttt tttttgaaac cggacctcaa ggtgcgcatc aactatataa
     8521 ccatacgcag caagcatgta aataccttga tatacgcacc gaggcaggca tagatatttg
     8581 tgaatatccc gactcgggaa agattaacat tcgaccgtat ggagaattgt atcaaagtga
     8641 agataaagtt gattattaca aaggagaaga taacgttcgt gaaaaatgga tgaatttatc
     8701 cgcaaagttt gtaaggaaat agtcatcccc ggcaatgaat g
//
LOCUS       DASVMB010000320         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_406972,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000320 DASVMB010000000
VERSION     DASVMB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406972"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1344
                     /locus_tag="VF531_09155"
     CDS             304..1344
                     /locus_tag="VF531_09155"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HEX7714179.1"
                     /translation="MTREELAAKTKPSLLEIAKSFGIKGTSRMTRNDLIEHILLLDPH
                     FSANPEQEKEISHQLESSLGNPGPNLTPPLPKHLTGVATYEAYEPEIPQNYNDTRIVL
                     MVRDPYWLYAYWNTNQYTREQISKTMRNWDQLRLMLRVYDITDIDFNGNNSNYYFDIE
                     LSHEANNWYIHVGKPNRTFCVDLGFIQENGSFFTIIRSNIVTTPRDNISDVIDEEWMV
                     IEEDFQKLYRLTGGGIGKSSAELVESLLKRLEREMGSGAVSSISSPSKLKPSERKFWL
                     VLNTELIVYGATEPDATVTVQGERIQLRPDGSFTLRFALPDGVQYIPVTARSADEVDA
                     ITITPVVSKQTS"
     gene            1353..2978
                     /locus_tag="VF531_09160"
     CDS             1353..2978
                     /locus_tag="VF531_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEX7714180.1"
                     /translation="MRLEKGYLAIVLHAHLPFVRHPEYPDFLEENWYYEAVTETYIPL
                     LKTFQELNESGIRYRITVSLSPPLVSMFKDPLLQQRYLKKIEQLIELAGKEVERTRWL
                     PQFHEVALMYLEHFRFCHGFFAEKCRCDLTTAFKELEQSGNVELITCGATHGFLPLML
                     NPAAVNAQIRQAVTYHTEVFNRRPRGIWLPECGFQPGVESALRDQGLRYFFTDAHGIL
                     HASPRPKYGVFAPIYTPSGVAAFGRDLESSKQVWSANEGYPGDYDYREFYRDIGYDLE
                     YDYIRPYLPSSGIRVATGMKYYRITGKTLDKQVYNPWIAREKAAEHAGNFMFNREKQI
                     EYIRSIIDRSPIIVAPYDAELFGHWWFEGPSWLKFLIQKITYDQQTIKLTTGSDYLEN
                     YPRNQVAVPSMSSWGYKGYNEVWLEGSNDWIYRHLHKLADRMAELAAAYPNAQGDLRR
                     GLNQAARELLLLQSSDWAFIMKTGTMVDYAVRRTKMHINRFNNLYDQIKYNRLELDYL
                     NQLESKDNIFPNLQYDIYLPESPKPVAEVALAT"
     gene            3293..3919
                     /locus_tag="VF531_09165"
                     /pseudo
     CDS             3293..3919
                     /locus_tag="VF531_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT         1157 a         1035 c         1044 g         1043 t
ORIGIN      
        1 tttatattaa aatttcgcgg cccaccggtt ttttcctgcc gggaattgcc tgtcgtttaa
       61 atgtcgtggt tgtgcgccat gtcgtggtta cgcgccttgg cgcgtaacca atgaaagatt
      121 cttaaagtgg agaaaaataa ccgtaacgag tcgtatataa gccacaaata ggggctatat
      181 tattaaagta cttgcccgcg aatcggatgc aaccgccaac cgttgaccca aagacccgaa
      241 acgccctggg tctggggacg aattggcatc ttttgcataa tttcctagga aagggggata
      301 gcgatgaccc gagaagaatt ggcggcaaaa accaaaccat cattgcttga aatcgccaaa
      361 tcttttggaa tcaaaggcac ttccagaatg acccggaatg atctaatcga gcatatcttg
      421 cttttagatc cacatttttc ggccaacccc gaacaagaaa aggagatatc tcatcaactg
      481 gaatcaagct taggaaaccc gggacccaat cttacgccac cgcttccaaa acacctgacc
      541 ggtgtggcga catacgaagc atacgaaccg gagatccctc aaaattacaa cgatacccga
      601 attgtcctca tggtccggga tccctattgg ctgtatgcct actggaatac caaccagtac
      661 accagggaac agatatccaa aaccatgcgg aattgggacc aactgcggct gatgctccgt
      721 gtgtatgaca tcaccgatat cgattttaac ggtaataaca gtaattacta tttcgatatc
      781 gagctaagcc atgaggccaa taactggtac atccatgtcg gaaagccaaa tcggactttc
      841 tgtgtagatt taggctttat tcaagagaat ggcagcttct tcacgattat tcgatccaat
      901 attgtcacta caccccggga taatatttcc gacgtaatcg atgaagaatg gatggttatt
      961 gaagaggatt ttcaaaagct ttatcgtctt accgggggag gcatcggtaa aagttcggct
     1021 gaactagtgg aatcactact caagcggctg gaacgcgaga tgggttccgg agcggtcagt
     1081 agcatctcca gtcccagcaa gctaaaaccc agtgaacgta aattctggtt agtcttgaat
     1141 accgagttga tcgtctatgg agccaccgaa ccggacgcga cagtcacggt tcagggggaa
     1201 aggattcaac tgcgaccgga tggctcattc accctgcgtt tcgccttgcc ggatggggtt
     1261 cagtatattc ctgtcacagc ccgatctgcc gatgaggttg acgccatcac cataacaccg
     1321 gtagtatcca agcaaaccag ttgaggagtt gaatgcgttt ggaaaaagga tatctcgcca
     1381 ttgttctcca tgcccatctc cctttcgtgc ggcatccgga atatcccgac tttctcgaag
     1441 aaaactggta ttacgaagcg gtcaccgaaa cgtatatccc gttgttaaag acttttcagg
     1501 agctgaatga atccggaatc cgctaccgga taacggtcag cctgtctccg ccgctcgttt
     1561 caatgttcaa agatcccctt ttgcagcaac gctatcttaa aaagattgag caattgattg
     1621 agcttgccgg aaaagaagtg gaacgcaccc gatggctacc ccagtttcat gaggtagcct
     1681 tgatgtactt ggaacacttt cgcttttgcc atgggttttt cgcagagaaa tgccgttgcg
     1741 acttgaccac ggcctttaag gaattggaac aaagcggaaa tgtcgaatta ataacctgcg
     1801 gcgccactca cgggtttctg cccctcatgc tgaacccggc ggcggttaac gctcaaatcc
     1861 ggcaagcggt cacctatcat actgaagttt ttaaccggcg gcctcgcggg atttggttgc
     1921 ccgaatgcgg tttccagccg ggagtcgaat ctgccttgcg tgatcaggga cttcgctatt
     1981 tctttaccga tgctcacggg atcctccatg cctcgccccg gccaaaatat ggggtatttg
     2041 cgcccattta tactcccagc ggggtggcgg ctttcggcag ggatttggaa tcctcaaaac
     2101 aagtatggag cgccaacgaa ggttatcccg gagattacga ttaccgggaa ttttatcgcg
     2161 atatcggcta tgatctcgaa tacgattaca tccggcctta cctaccttcg agcggaatcc
     2221 gtgtggccac cgggatgaag tactatcgga tcaccgggaa aaccctggat aaacaagtct
     2281 acaatccttg gatcgcacgg gaaaaggccg ccgagcacgc tggaaacttc atgttcaacc
     2341 gggagaagca gattgaatat atccgttcga tcattgacag atcaccgatt attgtggcgc
     2401 catacgacgc cgagctattc ggacattggt ggtttgaagg tccatcatgg ttaaagttcc
     2461 ttattcaaaa gatcacctat gatcaacaaa ccattaaact gacgacaggc tccgattatc
     2521 tggagaacta tccccgaaat caggtggcgg tcccgtcgat gtcgagttgg ggctacaagg
     2581 ggtataatga ggtctggctg gagggcagta acgactggat ttaccgccat cttcacaagc
     2641 tggccgaccg gatggcggaa ttggcagccg cctatccaaa cgcccaagga gatttgcggc
     2701 ggggattaaa ccaggcggcc cgggaacttt tactcctcca gagcagcgac tgggctttca
     2761 tcatgaaaac cgggacaatg gtcgattatg cggtacgccg taccaaaatg catatcaacc
     2821 gttttaacaa tctctatgat cagattaagt acaaccgtct tgaattggac tatttaaacc
     2881 aattggagtc caaggataat atctttccga atctccagta tgatatctat ttgcccgaat
     2941 cgccgaaacc cgttgctgaa gtagctttag ccacctaacc cgcgcgtcct ataggacgcg
     3001 aaaccctagc ctctcctcca ccagggaaaa ggacatcctt taatataatg aaagccaccc
     3061 atgataggtg gctttcatta tatctatctc gagtccattt tcgataggca actcgccata
     3121 tcgaactgta gccgccgcga attgcgcctc ctaagtgaac gtgatgtgac agccggagat
     3181 tcctgtgcgg gctttatttt gatgttcaaa tgctttataa tggattttaa aaaaactgac
     3241 tatgtgcaaa gtggagataa taagggcatt tatcgatcga agggggattg ctgtggacgt
     3301 tcaagcaatt ttacgggaac ttgaatcatt gggtacggag cgaaccaaaa aagtatatat
     3361 gcaacagggt gcgcgcgaac ctctttttgg ggtcgcaacg ggcgcgatga agcccatcgt
     3421 caagaaaatc aagaccaatc aggcgcttgc ggataagctt tacgctaccg gcaattatga
     3481 cgccatgtat ctcgccggag attagagacc caaaaaccat gaccgaagcg gattttgaca
     3541 gatggatgga aggcgcttat ttttatatga tttcagattt tgtcgtggcg gtgacgcttg
     3601 cggaaacaga ttttgcgcag gctgtttccg acagatggat tgcaaccggc aaaagctgca
     3661 tacgatgctc gaaacggtga agaaaacgat tcacagcagc cctgaccgcg ccaaatacgc
     3721 catgaataat tttgtcacaa ccttaggcgt ttcctatatt ccgctgcatg aaaaagcggt
     3781 atccgtcgca aacgccatcg gaactgtgga cgtgttcagg ggcaaagcca aatgcagtgc
     3841 ccctgtggcg gctgatgaga tccaaaaagc ggtggacaaa ggacggctgg gatttaagcg
     3901 taaaagcgtc aggtgttaga ggttgatttt ttgtaaatcg ccagccgatg tattataggt
     3961 gtactcccgc ttgcaaagtt ttgagccaat tcaacctcgt aaggcaggct gtctttggtc
     4021 ggcttcgcag ggagccctca ttctatcttt ctcccggggg gagaaacgaa ccgtccattc
     4081 tggaagttcc ggtaaaaaca aagcgcttaa actaaagcaa tgcccattta tgaatccagc
     4141 aacaactcat cgaactaagc ttctcgaatt ggccttcgtt ggggctgtct caaaagtccg
     4201 atttatcatt ccgtccaccc ggggttaaaa ccccgggcta agttaaaaac cattctaaag
     4261 cccgttaaaa cgggctggg
//
LOCUS       DASVMB010000321         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_115323,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000321 DASVMB010000000
VERSION     DASVMB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115323"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF531_09170"
     CDS             <1..410
                     /locus_tag="VF531_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714181.1"
                     /translation="WLVHIAYGIPMCTFFLRNFFAIIPISLMEASLIDGCSIAGYYRR
                     VLMPIAKPGLAALFILQFQSIWNDLLFGLAFSHGETVRPITVNLASFVGATDVQYGPL
                     MASTIVAILPMILVFLLFQREFIVGMLGGSVKG"
     gene            540..1982
                     /locus_tag="VF531_09175"
     CDS             540..1982
                     /locus_tag="VF531_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX7714182.1"
                     /translation="MRVIVGSLMHETNTFTIGTTSLNDFAPVIGDAIFKDSTWMGRQT
                     AAAGIIDKLRSEQIEVVPTLFGSALPSGTIQRQAYKTMKNEILTRTRRAMPVDGVCLA
                     LHGSMYVEGCDDPEGDLLSELRKLVGDEIPITCALDMHATITQAMVDSANALTAYQTA
                     PHVDKYETGERAAELLMKSLKHGTRLRMEWVKFPMLLAGEQSETNEQPMKDLLAMARD
                     KEQQTGISVSYMLGFPWADSPCNAVSVIAVGECSKDEVIKISAAELARKFWERRHDFQ
                     FTTEAYPLDEALTRALQDRKRPVIIADSGDNPTAGASEDLTLVVDAVVRRKLKEVLVA
                     VIADAKAYETCAKAGVGAMISLKLGRMTSRPDALPFITQVKVMQIQRAKGMNNAVVDI
                     DGVMVIITDTRCDVYDPTHLEELKLNPADYKIVIVKSGYLSPEYQRIAARKMLALTPG
                     DTAEIFSDLDYQKMKRPIFPLDQDTQWSIK"
     gene            2016..3263
                     /locus_tag="VF531_09180"
     CDS             2016..3263
                     /locus_tag="VF531_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7714183.1"
                     /translation="MDENDKRLQSLESVADKEIENIRDWIIETRRKVHADPELAFEEK
                     DTSELVKYTLQEAGWEIRPNSGNYGVMGVLHSRDNSGGKGKEYCVAFRADMDALPIQE
                     DTGVSYASRNPGKMHACGHDNHIAVALGIARVLAKLRGEWNGTVKLIFEPAEETGEGA
                     SLMIKGGAFENPKVDSIINLAVGTSISEGQFRLIEGLAMPQIDMFDINVEGESSHGGE
                     PHRGIDAIVLASEIVLVLQRVVSREVSVADPVILNVYQISGGHPDGIIPDHVTLKCTL
                     RSFTESARANALNRVRRAVKGVAESYGGKAAVEISKEAEFPVGSHDPAITRLVAEVAS
                     EVVGKKNVIYETKPDWGGDSFACYTQYAPGSFVWIGVRNEKEGRIYPVHHPKFNVNDQ
                     ILPNAVKVGCHVAFRILAGKLVS"
     gene            3447..4724
                     /locus_tag="VF531_09185"
     CDS             3447..4724
                     /locus_tag="VF531_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7714184.1"
                     /translation="MKKHGFTHVVIYGDREHFANVRFLTGYDPRFEETLLIIPMDKKP
                     ILMVGNEGLGYSKINQLDVEIEFFQHFSLQGQPRDRSKKMIDIFQGAGIAKESMIGVI
                     SNKYAESDDFGDRRYIIDAPAYIVDLLRQLSGSTKVVDVTYWFTHPQDGLRIPLTVEE
                     IVLYESANLLVYAGMKNALESLKPGITEIELASKLDYDGAFPLSCHISIGFGENALLG
                     LGSPTNRKLKKGDFVTIGFGVWGANIARTGIALSGPEELPQPIRDAMDKVYKPYFVGL
                     YNWYKALKIGVTGGELFDSMGKLATDPFFGMNLNPGHQLRDEEWINTPILSGSKLKLP
                     PQTMLQSDINVNAAAPYSGIHSEDGLVLADEKTRQQLAGRYPDVWERIVRRRKAMVED
                     FGYVLHDDVLPLSDMEGFVVPFLLNPKLALSLA"
     gene            complement(4840..>5084)
                     /locus_tag="VF531_09190"
     CDS             complement(4840..>5084)
                     /locus_tag="VF531_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7714185.1"
                     /translation="AAITPSTSCLIATRVDQGFQGRAYGIQQAARSLGSVFAPLMAGM
                     IGGWLGLRYVFIINALTGILFWISLRLQIKSPSSPK"
BASE COUNT         1291 a         1100 c         1386 g         1307 t
ORIGIN      
        1 tgtggctggt gcatatcgca tatggcatac ccatgtgcac ctttttcctg agaaacttct
       61 ttgcaatcat ccctatatcc ctgatggagg cgtcgttaat cgacggttgc tcgattgccg
      121 gatattaccg ccgggtttta atgccgatag ccaaacccgg cctcgcggcc ctctttattc
      181 tgcagtttca atccatttgg aatgatctcc tgtttggatt ggcgttttcc catggcgaga
      241 cggttcgccc aattaccgtc aacttagcat cgtttgtggg agctacggat gttcaatatg
      301 gaccgttgat ggcgtcgaca atcgtggcca ttttaccgat gattctggta tttctcttgt
      361 tccagcggga attcatcgtc ggtatgctgg gcggaagcgt caagggataa tcataaaacg
      421 tttttccctt gaaattattg gtgagagtag tccattcttt ttttgttttg aatatgtggt
      481 ttgtattttg agatgatcga ttcgttttga ttaaaaggtt agcgagagga atgatcttca
      541 tgagagttat tgtcggctcg ttaatgcatg aaacgaatac ctttacgata ggtactactt
      601 ctttaaacga tttcgctccc gttatcggag atgctatttt taaggattca acctggatgg
      661 gaagacagac tgccgcagcc ggaatcatcg acaaacttcg ttccgagcaa attgaggtag
      721 tcccaacatt atttggatcg gcgctgccat cggggacgat tcagcgccag gcttataaga
      781 ccatgaaaaa tgagatcctg acaagaacgc ggcgggcaat gcctgtcgac ggagtctgcc
      841 tggcacttca cggatccatg tatgtagagg gttgcgatga tccggaaggt gatctgctgt
      901 ctgaattgcg taagctggta ggggatgaaa taccgatcac ttgtgctttg gatatgcatg
      961 ccaccatcac gcaagcgatg gttgattccg cgaatgcttt aaccgcttac cagaccgctc
     1021 cgcatgttga taagtatgaa accggggaga gagcagctga gttgttgatg aagtctttga
     1081 aacatggtac ccgtcttcgt atggaatggg ttaagtttcc catgctcctc gcgggggaac
     1141 aatcggagac caatgaacaa ccgatgaagg atctattggc catggcacgc gacaaggagc
     1201 aacaaacggg gatatccgtc tcatatatgc tgggatttcc ctgggcggat tctccctgca
     1261 atgcggtgag tgtcatagcg gttggcgagt gctcaaaaga tgaagtaatt aagatatccg
     1321 ctgcagagtt agcccgaaaa ttctgggaaa gacggcatga ctttcagttt acaaccgagg
     1381 cttatcccct ggatgaggcg ttaacccggg cacttcagga ccgcaaacgg ccggtaatca
     1441 tagccgactc gggtgataat cccacggccg gtgcatccga agatttaacc ttggtagtgg
     1501 atgcggttgt ccgaagaaag cttaaggaag tattagtcgc ggtgattgca gatgcaaagg
     1561 cctatgaaac atgtgccaag gcgggagttg gagcaatgat ttctttgaaa ttgggcagaa
     1621 tgacctcccg tcccgatgct ctgcctttca tcacccaggt aaaagtgatg cagattcaaa
     1681 gagcaaaagg catgaataat gcggtggtcg atattgacgg ggtaatggtg atcattaccg
     1741 atacgcggtg cgatgtatat gatccgaccc atttggagga gttgaaactg aaccccgccg
     1801 actataagat cgtcattgtg aagagcggtt atctgagtcc cgaataccag cggattgccg
     1861 cccggaagat gctggccttg acgcctggag atacggcgga gatcttttcg gatctggatt
     1921 atcaaaagat gaaacggccg attttcccgt tggatcaaga tacgcaatgg agcattaagt
     1981 gaagagttct agcttaatta aggaggtttc atcatatgga tgagaacgat aagcggcttc
     2041 agtccctgga atccgtagct gataaggaaa ttgagaatat tcgtgactgg attatcgaaa
     2101 cgcgccggaa agtccatgcc gacccggagc tggcatttga agagaaagat acgtcggaac
     2161 tggttaagta tactttgcag gaggccggct gggagattcg ccctaattcc ggaaattacg
     2221 gcgtgatggg cgtgctccat agccgtgata atagcggtgg aaaaggcaaa gaatattgtg
     2281 tcgcatttcg ggcggacatg gacgctcttc cgatacagga ggatacgggt gtatcctatg
     2341 cgtcgcggaa tccgggcaag atgcatgctt gcggccatga caaccatatc gccgtggctt
     2401 tgggaattgc ccgggtcctt gccaagctgc gcggagagtg gaatggcact gtcaaactta
     2461 ttttcgaacc ggcggaagaa acgggcgagg gcgcatcatt aatgattaag ggtggtgcgt
     2521 ttgaaaaccc aaaggtcgac tcgatcatca acttggcagt gggaacctcc atctccgagg
     2581 ggcagtttcg tttgattgaa ggactggcta tgccgcagat tgatatgttt gatatcaatg
     2641 tcgagggcga atcctcccac ggcggagaac ctcatcgcgg tatcgacgcc attgtgcttg
     2701 cttcggaaat cgttctggtt ttgcagcggg tagtcagcag ggaggtatcc gtcgccgatc
     2761 cggtgatatt gaatgtttac caaatttccg gcggacatcc ggacgggatc attccggatc
     2821 atgtaacctt aaaatgcact ttgcgttcat ttaccgaatc cgccagagcg aacgctttga
     2881 accgggtacg acgggcggtg aagggagtag ccgagagcta tggaggaaag gcggcggttg
     2941 agatatcgaa ggaagccgaa tttccggtgg gttctcatga tccggcgatc acccggcttg
     3001 tcgccgaagt ggcatcggaa gtcgtaggta aaaaaaatgt gatttatgaa acgaagcctg
     3061 attggggcgg cgattcgttt gcctgttata ctcaatatgc tccgggatct tttgtctgga
     3121 ttggggttcg taatgagaaa gaaggccgta tttatccggt tcatcatcca aagttcaatg
     3181 tgaatgacca gatattgccg aatgccgtta aagtcggttg tcatgtcgct ttccgtatat
     3241 tagccgggaa actcgtaagt taaaggaata tgattgatgg agtttataca attatgacga
     3301 ggagaatcaa gatgactctc aaaaaaccta ttgcatccca aattgaatta aaatcagtta
     3361 aatatcctga agcagtaatc cccaccgaat tttctgcaat acccaagagc gaatatgaaa
     3421 gtcgtctcca gaaggtgcgc ggattgatga agaaacacgg tttcacccat gtcgtgattt
     3481 atggggaccg ggaacatttt gccaatgtaa ggtttctcac cggttatgat ccgcgttttg
     3541 aagaaacttt gttgatcatt cccatggata agaagcccat tttgatggtt ggcaatgaag
     3601 gactgggata tagtaagatc aaccagttgg atgtggaaat tgaatttttc cagcatttta
     3661 gccttcaagg acaaccccgg gatcgcagca agaaaatgat cgatatcttt cagggcgccg
     3721 gaatcgccaa agaaagcatg attggcgtaa tctccaacaa atacgcggaa agcgacgact
     3781 tcggcgaccg cagatacatc attgacgctc cggcctatat tgtggacctg ttgcgccaac
     3841 tctccggatc aaccaaagtt gttgatgtta catactggtt cacccatcct caggatggtc
     3901 tcaggatacc gttgaccgtg gaagagattg tattgtatga atcagccaat ctcttggttt
     3961 acgccggaat gaaaaacgcc ctcgaatcct taaaaccggg aatcaccgag attgagctgg
     4021 cctcaaaact ggactacgac ggcgcctttc ccctttcctg ccatatctcc atcggtttcg
     4081 gggagaatgc cttattgggg ttaggcagcc ctactaaccg taaattgaag aagggtgatt
     4141 tcgttaccat cggttttggt gtatggggtg ccaatattgc ccggacggga attgccttgt
     4201 cgggtccgga ggagttgcct cagccgatcc gggatgctat ggataaagtt tataaaccat
     4261 attttgtggg cttatacaat tggtacaaag cgttaaaaat cggtgtgacc ggtggggaac
     4321 tctttgacag catgggtaag ttggctaccg atccattttt cggtatgaac ttgaaccctg
     4381 gtcatcaact tcgggatgag gagtggatta ataccccgat cttgtccgga tcgaaactca
     4441 agttgcctcc gcagacaatg ctgcaaagcg atatcaatgt caacgccgcc gccccatact
     4501 caggcattca ttcggaagac ggactggtac tggcggatga gaaaacacgg caacaactgg
     4561 ccggccgtta cccggatgtt tgggagcgca ttgtgagaag gcgcaaggcg atggtggaag
     4621 atttcggtta cgtccttcat gatgatgtat tgcctctgtc ggacatggaa ggattcgtag
     4681 ttccgttttt gctcaatccc aagttggctt tgagtttggc ctaagtcccg gccggaaatt
     4741 tggccttgaa aatggaaatt tagattgcga tgtcaatgcc acctgatttt ttaggtggca
     4801 ttgatgattt taaggcattc agggatattc ttgaatcgtc tatttcgggg aagacgggga
     4861 tttaatttgt aaacgcaggc tgatccagaa caggattccg gtcagggcgt tgatgatgaa
     4921 cacataccgc agtcccagcc agcctccgat catccccgcc atgagggggg caaaaaccga
     4981 acccagactc cgggctgcct gctgaatccc gtaggctcgt ccttgaaaac cctgatccac
     5041 ccgggtggca ataaggcagg aggtggaggg ggtgatggca gcga
//
LOCUS       DASVMB010000322         6481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_262381,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000322 DASVMB010000000
VERSION     DASVMB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262381"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1231
                     /locus_tag="VF531_09195"
     CDS             2..1231
                     /locus_tag="VF531_09195"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HEX7714186.1"
                     /translation="MLAVPERTGFFFDDQVAIKSGAEPDGFTYRGQPVLPGFSAVRQA
                     GEAVGILLILENGAIAHGDCAAVQYSGVGGRDPLFLAAQFVPVINRLIKPRLEGRELT
                     GFRDLAAEFDSLVDPETGRILHTAIRYGITQALLDAVAQAHGRMMAEVVAAEYHTRIA
                     DRPIHIFAQSGDERYTNVDKMIIKQVPVLPHGLINNVTNKLGRNGELLLEYVNWLKNR
                     VARLRPRPDYAPILHIDVYGTIGLAFDDDYQRILDYLGRLAEAAAPLKLRIEGPVDMG
                     GQAAQIEMLALLRRGCSEKGIDVALVADEWCNTLEDVKLFASNQAGHMIQIKTPDLGG
                     INNSIEAVLFCKSHQIGAYLGGTCNETDRSAQICVHVAMATEPDQILAKPGMGVDEGY
                     MIVYNEMQRILALVAAR"
     gene            1261..2652
                     /locus_tag="VF531_09200"
     CDS             1261..2652
                     /locus_tag="VF531_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX7714187.1"
                     /translation="MEELRILSPTAILGYGFPERSFVEGMGRKPDLLAVDAGSTDPGP
                     YYLGAGVSFTEYRAVKRDLTIMLQAALEAGIPVFIGSAGGSGGRPHLEWNLRIIREIA
                     HEKRLHFRLAAIEAEIPRETVTSLWQQHRLKPLPPAPDLSSADIEETERIVGQMGVEP
                     FLRAMESGAQVILAGRSYDPAVFAAYGIARGFDPGLAIHLGKILECAAIAATPGSGSD
                     CLMGYLHRSDFEVEPLNPARRCTPLSVAAHTLYEKSNPYELPGPGGVLDLHAARFDAV
                     TENRVRVTGSRFLPTSYQVKLEGVKRTGYRTVSIAATRDPLMIREIDQVVSGVRERVT
                     DNFKAEGFDYFLDFKIYGRNGVMGSLEPEPVIGSHELGIVIEAVAASQELADTVCGFA
                     RSTMLHFGYPGRIATAGNLAFPYSPSDFPAGPVYVFSIYHLAAVTAEELFPVTVREID
                     PDGRADIVEEAIR"
     gene            2649..2966
                     /locus_tag="VF531_09205"
     CDS             2649..2966
                     /locus_tag="VF531_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HEX7714188.1"
                     /translation="MRVLLTSIAEVIRSKNSGPFELTLDIIFKERAVYEKVCSAGEFT
                     PERIAALYGISPRTVLAIIEFEPAKAIKINLKRPIPSGGLGDTDIYGAQQHAPLLGME
                     LEL"
     gene            2995..3921
                     /locus_tag="VF531_09210"
     CDS             2995..3921
                     /locus_tag="VF531_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-tRNA synthetase second additional
                     domain-containing protein"
                     /protein_id="HEX7714189.1"
                     /translation="MAESITSESLMYAVYFAPRGKRRLLNLGHQLGQRHLNPLDKLIG
                     VIGDAGAGKSLLINGMFPGLELTNDDSGINIRPLPLLDQSANGAFTHHTYHVDIRFEM
                     AFTPAYQLAEAIRQAVASGRRVIVEHFELIYPLLAMNAEILIGLGEEVLVTRPNIFGP
                     LPDDIAAIVFKSVRYRRMAHSAEDLTELVLRRDFGFTGPLSHDDVKHGFVLEFTAKPE
                     LDLVRVEERVMSYIEQDIPIQYHDDSHVALGSELVPCTGPRLHVRNTGAIQNFQLIKE
                     FKYDPIAKLYLLVGLVGSDHPLNLNDLNDLTV"
     gene            3933..5315
                     /gene="glmL"
                     /locus_tag="VF531_09215"
     CDS             3933..5315
                     /gene="glmL"
                     /locus_tag="VF531_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HEX7714190.1"
                     /translation="MRPYLLIDFGSTYTKMTAVDLDEPKVLGTSRALTTVQDGLLVGY
                     RRALEQLHQRIGILEYQKRLACSSAAGGLKMAAVGLVPELTVEAAKRAALGAGARVIR
                     TYGYELTPDEVQELVGLQPDLVLLAGGTDGGNARVLLHNADQLSKSVLATPIILAGNK
                     SAAPEAEKILTSAGKECYRVANVLPELNRLNIEPVQQTIRELFFKRIIHAKGLDQVER
                     LIDGITMPTPAAALDAATLLADGPEGRGGLGELLLIDVGGATTDVHSVAQGAPTDPQV
                     SLRGLPESRVKRTVEGDLGMRYSAQALLETVSAGAVAKRSGLPEGTVIEGVTRRSRNV
                     TYLPQDQEDWLLEAALGYLAVAGAVERHVGRIEKFYTPSCLSYIQTGKDLTRLPVLIG
                     TGGILAHSSAPGEILRGALFDPVRPEFLKPQAPKLWFDRDYLLPTLGLIAKTHPQAAF
                     SLLKQALHPA"
     gene            5485..6000
                     /locus_tag="VF531_09220"
     CDS             5485..6000
                     /locus_tag="VF531_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714191.1"
                     /translation="MVFALDGSGEFARIQNRFITSMGQYVESYGGSEIMGRIIGLLLF
                     APEPVSLTEIAKSLQISKAAASINMKYLRQMKAVEKVTLPGDRGDYYRMDEDYGYGVF
                     SSSFRKKIQDGLELIGDALLDLDGLGERKSIATAAIARERLQQMRELYQGLGKLYDEL
                     RNDWDEKKLKK"
     gene            6014..>6481
                     /locus_tag="VF531_09225"
     CDS             6014..>6481
                     /locus_tag="VF531_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7714192.1"
                     /translation="MSVIFAKDVIKEYPHGSTTVPALRGIDLTVETGEFTSLAGPSGS
                     GKTTFLNLIGCLDKTSSGKLLIDGTDVATLSPIELAALRREKIGFIFQTYNLIPVLTA
                     YENVELPLVLLRNETESRIKERVYQALADVGLKGLEQRRPADLSGGQQQRVAIA"
BASE COUNT         1445 a         1604 c         1886 g         1546 t
ORIGIN      
        1 actgcttgcg gttccggaac ggaccggatt tttcttcgac gatcaggtgg ccatcaaaag
       61 cggggcggag ccggacggct ttacctatcg cggccaaccg gtcttaccgg ggttttccgc
      121 cgtacgccag gccggggaag cggtagggat cttgttgatc ctggagaatg gcgccatcgc
      181 ccatggtgat tgtgccgccg tccaatactc gggagtcggc ggcagggatc cgttgttcct
      241 ggccgcgcag ttcgttccgg tgatcaaccg gttaatcaaa ccccgtcttg aaggacggga
      301 gctgaccgga ttccgcgatt tagccgcgga gtttgactca ctcgtcgatc cggagaccgg
      361 ccggattctc cataccgcga ttcgatatgg gataacccag gcgttactgg atgcggttgc
      421 tcaagctcac ggacggatga tggcggaagt agtcgctgcc gaataccata cccggatagc
      481 cgatcgcccg attcatatct ttgcccaatc cggcgatgag cgctacacca atgtggataa
      541 gatgatcatc aagcaagtac cggtactgcc ccacggttta atcaacaatg tcaccaacaa
      601 gttggggcga aacggtgagt tgctccttga gtatgtgaac tggctcaaaa accgggtcgc
      661 gagattgcgg ccccgaccgg attatgcgcc tattttacat atcgatgtat acggcaccat
      721 cggtttggct tttgatgatg actaccagcg aattctggat tatttgggac gtttggctga
      781 ggctgcggcg cctcttaaat tgcggattga gggtccggtg gatatggggg ggcaagcggc
      841 ccaaatcgag atgctggcat tgttgcgacg gggatgtagc gaaaaaggta ttgatgtggc
      901 attggttgcg gatgaatggt gcaataccct ggaagatgtg aagctttttg cttccaatca
      961 agccggccat atgattcaga ttaaaacccc cgatttggga ggcatcaata actctattga
     1021 agctgtgctt ttctgtaaat ctcatcaaat cggtgcttat ctcggcggga cctgtaatga
     1081 gacggatcgc tccgcccaaa tctgtgtcca tgtggcaatg gcgacggagc ccgatcagat
     1141 cctggccaaa ccggggatgg gagtcgatga aggctatatg atcgtctaca acgagatgca
     1201 aaggattttg gccctggtag cggcgcgcta aagcgcgcct cagaaggagg aaaatataaa
     1261 atggaggagc tccgaatcct gtctcccacc gcaatcctgg gttacggttt tcccgaaaga
     1321 tctttcgtag aggggatggg gcgaaagccg gacctgcttg cggtggacgc gggttctacc
     1381 gacccgggtc cctattatct cggagccggt gtttccttta ccgaataccg ggctgtgaaa
     1441 agggatttaa cgattatgct ccaggcggcg ctggaagcgg gaattccggt gtttatcggt
     1501 tcggccggcg gcagcggggg cagaccccat cttgagtgga atcttcgcat cattcgggag
     1561 atcgcccatg aaaagcggct gcattttcgc ctcgccgcca ttgaggctga aatcccccgg
     1621 gagactgtga cttccctctg gcaacagcat cgtctcaaac ccttacctcc ggcccctgat
     1681 ttgagtagcg ccgatattga agaaacggag cgaatcgtcg gtcagatggg ggttgaaccc
     1741 ttcctccggg caatggaatc cggcgcccag gtgattttag caggccggag ctacgatccg
     1801 gcggtgtttg cagcctatgg gatcgcccgg ggttttgatc cggggcttgc aattcacctc
     1861 ggcaagatcc tggaatgcgc agcaatcgcc gccactcccg gtagcggcag tgactgcctc
     1921 atggggtatc tgcaccggtc ggatttcgag gtggaaccgc tcaatcccgc ccggcgctgc
     1981 acccccctgt ccgtggcagc gcatacatta tatgaaaagt ccaaccctta tgaactgccc
     2041 ggtccggggg gagtcctgga tctccacgcc gcccggttcg atgcggtgac ggaaaaccgg
     2101 gtccgggtga ccggcagccg gtttttacca accagttacc aagtaaagct cgaaggggtg
     2161 aaacgaaccg gttaccggac cgtgtccatc gccgccaccc gtgatccatt gatgatcagg
     2221 gagatcgacc aggtggtctc gggggtgagg gaacgggtta ccgataattt taaggctgaa
     2281 ggattcgatt attttctgga ctttaagatt tatggccgaa atggagtgat ggggtccctg
     2341 gaaccggagc ccgtaatcgg tagtcacgaa ttggggatcg tcatcgaagc ggtcgctgcc
     2401 agccaggagt tggccgatac ggtatgcggc tttgcacgtt caaccatgct gcatttcggt
     2461 tacccggggc ggattgcgac agccggaaat ctggcatttc cttattctcc ctccgacttt
     2521 ccggcaggac cggtttatgt cttctctata taccatctgg ctgcagtcac ggcggaggaa
     2581 ttgtttccgg tcacggtccg tgagatcgat ccggatggcc gggccgacat tgtcgaggag
     2641 gcgatacggt gagagtgctc ttgactagta tagctgaagt catccggagt aaaaattccg
     2701 gcccgtttga gctcactcta gatatcatct tcaaggaacg ggctgtttac gaaaaggtct
     2761 gtagtgcggg ggagtttacc ccggagcgaa ttgccgcctt atatggaatc agcccccgga
     2821 cggttttggc aatcatcgag tttgaaccgg ccaaggctat caagatcaat ctaaagcgac
     2881 cgataccctc gggcggattg ggcgataccg atatctacgg tgcccagcaa catgcgccgc
     2941 tcctcgggat ggagttggag ctatgaatct attgaaatgc gggtgatacc caaaatggca
     3001 gagagcatca cctccgagtc attgatgtat gccgtgtatt ttgcaccccg gggtaaaagg
     3061 cggcttttga accttggcca tcaattgggc caacgccact tgaaccccct cgataagtta
     3121 atcggggtaa ttggggatgc cggtgccggt aagtctctac ttatcaatgg tatgtttccg
     3181 ggattggaat tgaccaatga cgactcggga attaatatcc ggccgttgcc gctattagat
     3241 caatccgcga acggtgcctt cacccaccat acctatcatg tggatatccg ttttgaaatg
     3301 gcgtttacac cggcgtacca gttggcagag gccatcaggc aggctgtcgc cagcggacgc
     3361 cgggttatcg tagaacattt cgaattaatc tacccgctgc ttgccatgaa tgcggagatc
     3421 ctcatcggcc tcggagaaga ggtcctggta accaggccta atatattcgg gcctttaccc
     3481 gatgatatcg cagccattgt cttcaagtcc gtgcgttacc ggcgcatggc gcatagtgcc
     3541 gaggatctca cggaattggt actacggcgc gacttcggtt ttaccggtcc gctttcccat
     3601 gatgatgtta aacatggttt tgtgcttgag ttcactgcca agccggagtt ggatctggtc
     3661 cgggtggaag agcgggtgat gagctatatt gagcaggata tccccattca ataccatgac
     3721 gattcccatg tggcgttagg ctccgaattg gtcccatgca ccgggccgcg attacatgtc
     3781 cggaatacag gggccatcca aaacttccaa ctaattaagg agttcaagta cgaccccatc
     3841 gcaaaactct atcttttggt aggactggtg ggaagcgatc acccgttgaa cctgaacgac
     3901 ttaaatgatc ttacggttta gcggaggttg acatgcgtcc gtatttgttg atcgattttg
     3961 gcagtactta tacaaagatg actgccgttg atctggatga acccaaggtt cttggaactt
     4021 cccgggcttt gaccacggtg caggacggat tgctcgtcgg ttaccggcgg gcgttggaac
     4081 aattgcatca gcgcattggg attttggaat atcagaaacg cctggcatgc agcagtgccg
     4141 cgggcggcct aaagatggcg gctgtcggat tggtgcccga acttacggtg gaggcggcca
     4201 aaagggcggc cctgggtgcg ggggcccggg ttattcgaac ctatggttac gagcttactc
     4261 cggatgaggt gcaagagctg gtcggattac agccggatct ggtattgctt gccggaggga
     4321 cggacggcgg taatgcccgg gttttgttgc ataacgccga ccaactgagt aaaagtgttc
     4381 ttgcgacacc gattattttg gccgggaata agtcggcggc accggaagct gaaaagattt
     4441 taacctcagc cggcaaggaa tgttaccggg tggccaacgt tttgccggaa ctgaaccggt
     4501 tgaatattga accggtccag caaaccatcc gcgagttgtt tttcaagcgg atcattcatg
     4561 ccaaaggttt ggatcaggtg gagaggttaa ttgacggcat caccatgccc actccggcag
     4621 ctgctttgga tgccgccacg ctgctggccg atggtccgga aggcaggggg ggtttaggtg
     4681 aacttctcct gatcgatgta ggtggagcta ctaccgatgt gcattccgtg gctcaaggtg
     4741 cgccaacgga tccacaggtt tctttgcggg ggctgccgga gtcgcgggtc aaacgaaccg
     4801 ttgaagggga tctgggcatg cgctacagtg cccaggcctt gttagaaacg gtttcggccg
     4861 gagcggtagc caaacggtcc ggccttcccg aaggtacggt aattgaggga gtcacccggc
     4921 gcagtcgcaa cgtgacctat cttccccaag atcaagaaga ttggctttta gaggctgctc
     4981 tgggttatct ggccgtcgcc ggagccgttg aacggcatgt gggccggatt gaaaagtttt
     5041 ataccccaag ctgcctttcc tatattcaga ccggaaagga tctgacccgc ttgccggtgc
     5101 tgatcggtac cggcggaatc ctggcccatt cgtccgcacc cggtgaaatc ttgcgtggtg
     5161 ccttatttga tcccgtccgc ccggagtttc tgaaaccgca agctcccaaa ctttggttcg
     5221 atcgggacta tctcctgccg acactgggtt taattgccaa gacccatcct caagcggcgt
     5281 tttccctttt gaaacaggcc ttgcacccgg catagagact aagaatcaag atgggtatta
     5341 ggcttccggc ggttgttacg aattggttgc agccactctt atcccattga ctcttttgcc
     5401 gttgggacta aaatagagat atcgttaatg aaattattaa cgttatgaat gatttaaaca
     5461 ttcatcactc tggagaagcc gggggtggta tttgccttgg atggcagtgg agagtttgcc
     5521 cgtattcaaa accgtttcat cacatccatg ggacagtatg ttgagtccta cggtggcagc
     5581 gagatcatgg gccggatcat tgggttattg ctttttgcac ctgagccggt ctccttgacc
     5641 gaaattgcca aaagcctgca gatatcaaag gctgcggcca gtatcaacat gaaatattta
     5701 cgtcagatga aagcggtcga gaaggttacg ctccccggtg atcgcggtga ttactatcgg
     5761 atggacgaag attacggcta cggcgttttt tcaagctcat tccgcaaaaa aattcaggat
     5821 ggattggaat tgattggcga cgccttactg gatttggatg gactgggtga acggaaaagt
     5881 atcgctactg cggccatcgc ccgcgaacgc ttgcaacaga tgcgggaact gtaccaggga
     5941 ttaggtaagc tctatgatga gctgagaaat gactgggatg aaaagaagct taaaaaataa
     6001 ggatgtgatc aggatgagcg ttattttcgc aaaggatgtt atcaaggaat atccgcatgg
     6061 ttctacgacc gtcccggcgt tacgcgggat cgacctgact gttgaaacgg gtgagtttac
     6121 gtcgctcgcg ggaccttccg gttccggcaa gaccacattt ttaaacttaa tcggctgtct
     6181 tgataaaacc agttccggaa agcttttgat cgacgggacc gatgtggcga ccctttcccc
     6241 tattgaactg gcggcgttgc gccgtgagaa gatcggcttc atctttcaga catataattt
     6301 gataccggta ttgactgcct atgaaaatgt ggaactgccg ctggtattac tcagaaatga
     6361 aaccgaatcc cggatcaagg aacgggttta ccaagcctta gctgatgttg gactcaaggg
     6421 acttgagcaa cggcggcctg cggatctctc gggcggtcag caacaacggg tggccatcgc
     6481 c
//
LOCUS       DASVMB010000323         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_555681,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000323 DASVMB010000000
VERSION     DASVMB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555681"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VF531_09230"
     CDS             complement(<1..1323)
                     /locus_tag="VF531_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714193.1"
                     /translation="MKLRFWFFFTLLLSVLMLPIPIFSTGNHERKQVIILDIPRFDLA
                     EITPEYPFLNKFIANSSVGLVTIPSIDYPLKSGQIYLYFNSNSFLKTPPEGYGIYDYT
                     EKVNGIRAADLHQTFSGKPALPGNAINIDLEKLLQSNTNLTNSNLGYFGNTLHDLLLK
                     TAVIGNADTERPNRSGAAMAMDSEGIIDYGAVGKETIQKDRLFPSGGRTNLQRVIQYY
                     RRIRSKADLIVITLGDLERIESYTRLLNPSQKEFYRRQTLRRYDGLCRDLLGSIPSRP
                     AMIVVFTCLQPDKPHIKNNGLTPVIIHGTDFPNGLLYSRSTRKKGHITYPDLRNTILA
                     YLSVPIDNRRQAVAVMPGDWQNLIIEQLGLVTDYSFRWPLLTTYIALLVGLTCVIIIG
                     LILHFKIAFLKAIGWCYLWLLTIPATFLLEGLASPTQWRWVILYTLGIS"
     gene            complement(1726..3471)
                     /locus_tag="VF531_09235"
     CDS             complement(1726..3471)
                     /locus_tag="VF531_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7714194.1"
                     /translation="MLLFSGEKLEKNFGECEVFRNISFEITRLERVGIVGPNGVGKTT
                     LLKVIAGEESPSAGLLRFFSPVSISVLNQYCPFPSALQVEQAVQNAAPSGSPVSTEEI
                     LKKFGFANLGDRKIGDLSGGEKTRLQLACIWREPADLLLLDEPTNHLDLVQLDWLEGF
                     IREYPGTIILVSHDRYFLDRTVSRVLELSPTGMASFPGNYTNYQRIKAEQRARDERLF
                     AEQARQAVKLEKAITEQVQWSAKAHRESAEKARAMGRKKGGKEYLRAKAKKIDRRVNN
                     TVKRLERLRREKITKPATMETIDLSFRDAGRRGRQSLITGWGLTKSYDGRELFDSVSL
                     TLQPGEKIGLIGRNGSGKTTLLKILLGLEQPDSGNVWSSPALQAGYLDQEAQSLDLEK
                     TVLTELVDVCSDPGRARKLLAGLLLQGDTVYKQCASLSMGERVRVELAKLLLGSYDLL
                     MLDEPTNYLDLESRERLEEALNDFSGALIIVSHDRYLLERVCNKIWSLENRRLRIFPG
                     KYSEAVASGSRPEQTTDREAEKLRLGFKMADITGRLSLIDRGRNPEEYGILEAEYFLL
                     VEKYQTLTRNSKGKG"
BASE COUNT         1029 a          902 c          929 g         1026 t
ORIGIN      
        1 ggaaatacct aaggtataga gaatgaccca acgccactgg gtgggactgg ccaaaccttc
       61 gagcaggaat gtggcgggaa tggtcaagag ccatagatag caccatccta tagcttttaa
      121 gaaggcgatt ttaaaatgca aaataagtcc gataatgatg acgcatgtca atccgacaag
      181 tagcgcaata taagttgtaa gcaagggcca acggaaggaa tagtcagtca cgagtccaag
      241 ttgctcaatg atgaggtttt gccaatcacc gggcattacc gcgactgcct gccggcgatt
      301 atcgattgga accgataaat aggcaaggat tgtgtttcgg agatcagggt aggtgatatg
      361 tccttttttc cgagtactcc tcgagtataa caaaccgttt ggaaagtcgg ttccgtgaat
      421 aatgacgggt gttaatccat tgttttttat gtgaggttta tcaggctgta aacaagtgaa
      481 tacaacaatc atggcaggcc tcgaaggaat ggaacccaat aaatctctgc ataaaccatc
      541 gtaacgccgc aaagtttgtc tgcgataaaa ttctttttgg gaaggattca acagacgggt
      601 atagctttca atccgttcca aatcccccag ggtaattacg atgagatccg ccttacttcg
      661 aatacgccgg taatattgga taacccgttg taaattggtg cgaccgccgg aaggaaaaag
      721 ccggtctttt tgaatggttt ctttcccgac cgcaccgtag tcgataatcc cttccgaatc
      781 catagccatt gccgccccgg atctgttcgg cctctccgta tcggcattgc cgatgacggc
      841 agttttaagc agaaggtcat gtaaggtatt gccgaaatac ccaagattgg aattggtgag
      901 gttggtgttg gattgtaaga gtttctccag atcaatattt attgcatttc cgggaagagc
      961 gggtttaccc gaaaaggttt gatgaagatc agccgcgcga attccattga ccttttcagt
     1021 ataatcatag attccgtaac cctccggcgg agttttcaaa aaactgttac tattgaaata
     1081 taagtatatc tgtcctgatt ttagcgggta atcgatggag ggtatcgtaa ccaaacccac
     1141 cgaactattc gcaatgaact tgtttagaaa tggatattcc ggagtgattt cggccagatc
     1201 gaaacgagga atatcgagga tgattacttg ttttctttca tggtttccgg ttgaaaaaat
     1261 cggaatcgga agcatgagta ccgataaaag cagggtgaag aaaaaccaaa agcgtaattt
     1321 catagatggc tccttagcgg gacgaataaa acaccgtcta aaaataagag acatttcgtg
     1381 tatttggtga ttttcgtggt taaaaagttt ctatcggtaa gcctatttta ttcacaacga
     1441 aacacacaaa agaaacgaaa atgggaatag ctttagttgc agccattgaa gtatttatct
     1501 atcataaaca aaaaaggact ccgtagatca accattattg aaaataaagc gaaatctttt
     1561 aattattctc tttggaaaag gtataacctg ttcgtatcat aaaatttaat ctagtaggcg
     1621 agggtataag ttttgaaaaa tttttgaaga ggagcatttc atgccccgat tggaggtgga
     1681 gatcgagcaa acgctcattc cttttattat ttttgacttt tgactttacc cttttccctt
     1741 agagttccgg gtgagggttt gatacttctc taccagcaag aagtattctg cctccagtat
     1801 cccatactct tcagggtttc tcccgcgatc aatcaaggat aagcgtccgg taatatcggc
     1861 cattttaaat cccaatctga gtttttcggc ttcacgatcc gtcgtttgct ccggccggga
     1921 acccgaggcg accgcttcag agtatttacc gggaaagatg cgaagtctcc ggttctcgag
     1981 ggaccagatc ttattgcaga cacgttccaa cagataacgg tcatgggaga cgatgatgag
     2041 ggcgccggag aaatcgttca aggcctcctc gagccgttca cggctctcca gatccaggta
     2101 gttagtgggt tcatccaaca ttaacaggtc ataggatccg aggagcagct tggccagttc
     2161 cacccggacc cgttccccca tactcaggga agcgcattgt ttgtaaaccg tatcaccctg
     2221 aagcagtagg ccggcaagga gcttccgggc cctgccggga tcggaacaaa catcaaccag
     2281 ttcggtgaga acggttttct caaggtcaag cgactgcgct tcctggtcca gatatcccgc
     2341 ctgtagcgcc ggggaagacc agacgttacc ggaatcgggc tgctccaaac caagcaatat
     2401 cttgagcagg gtagtttttc cggaaccgtt acgcccgata agaccaatct tttcaccggg
     2461 ctgaagggtg agtgagacgg aatcgaagag ctcccggcca tcatatgact tggtaagccc
     2521 ccagccggtg ataaggcttt gccgtccgcg ccgtcctgca tcccggaacg agagatcgat
     2581 cgtctccatg gtcgccggtt tggtaatctt ctcccggcgc agtctttcca gacgtttcac
     2641 ggtgttgttg acgcggcggt cgattttttt ggctttggcg cgcaaatatt ccttaccgcc
     2701 cttctttctc cccatagccc tggctttttc tgccgattcg cgatgagcct tggcagacca
     2761 ttggacctgc tcagttatgg ccttttccaa ctttacggcc tgcctcgctt gttcggcaaa
     2821 gagcctttca tcccgagccc gctgttcggc tttgatccgt tggtagttgg tatagttgcc
     2881 cgggaatgaa gccatgccgg tagggctgag ctccagaacc cggctgacgg tgcgatccag
     2941 gaagtaacga tcatgggaga ccagaatgat tgtaccggga tattcccgga taaatccctc
     3001 cagccaatca agctgaacca gatccaagtg attggtgggt tcgtccagaa gcaacagatc
     3061 ggcgggttct ctccagatac aggccagttg cagccgggtt ttttcgcctc cgctcaaatc
     3121 gccgatcttc cggtcaccta agtttgcaaa accgaatttt ttcagaatct cctcggtgga
     3181 taccgggctt ccggagggag ccgcattttg cacggcctgc tctacctgga gagcagatgg
     3241 aaaagggcag tattgattga gaacggaaat tgaaaccgga ctaaagaaac gtagcaatcc
     3301 ggcggaaggg gattcttccc ctgcgatcac ctttaaaagc gtggtttttc caacaccgtt
     3361 cggaccgacg atgccgaccc tttccaaacg ggtgatctca aaggatatat tgcgaaagac
     3421 ctcacattct ccgaagtttt tttcaagttt ttcacctgaa aacaaaagca tccataccac
     3481 tccttcatgg taggatgctc cacgccgttt ttatcttcat tctccaggga tttttcataa
     3541 aacccggttt ctaccgatat ctcctggatc caaatatctc ctgaatccaa aaaaggagag
     3601 tcggtaaaac atgatctttt aaaaccggat gtgccggatt tattaggagg aggataacaa
     3661 aaaaccgtcg tcaggaaatg taacatccta ccgtaatgta gaactcaggc tttttacggt
     3721 ttggaatgct actatctcct cattggcggc tcctttttgc ctatttttac accggatata
     3781 ttccctattc cgggccgaag cgtgcgtgcg cgacgcttcc ggggttattt ataagcaaat
     3841 taattttacc atgaaaaaac cgatctgcca agcagaaatc ttggat
//
LOCUS       DASVMB010000324         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_705271,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000324 DASVMB010000000
VERSION     DASVMB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705271"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VF531_09240"
     CDS             <1..1551
                     /locus_tag="VF531_09240"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7714195.1"
                     /translation="EMVGSRLLAPTFGSSIYIWGSLIVVVMTALTLGYYYGGRIADKF
                     PNYRVMGLVLACAGVYIGFLPFWAKSMSDSVITLEPRLGSLLVSMVFFFVPSVLLATI
                     SPFGIKLSSRNLTTIGNTAGFMSAISSGGSILGTLLTSFFLIPVMGVRNIVHTLGIIL
                     LVLALVVFLVARRHTGSDSGENGRAALKGTNIMICLVILGGLSLSGLWVLASVRVGEE
                     WGYKTLYERDSLYHHIRVVQDTNWRRLHFDESFQSEMDLENPLEMAFLYTSYLHVGVI
                     AHPQPSRVLFIGLGGGSAPKKFLNDYPSLKAVDVVEIDPEVVKVARKYFQLPDDPRLR
                     IFAQDGRIFVEKTAREIAAGKTDPYDMVIIDAYTASSIPYHLTTREFVAAVRKTLSRD
                     GVVVSNIIGAFAGPASPLLSSMTRTIKSVFPQTYLFPTWNWEGKNDYAENNVILVATA
                     DSRYWDAATWRKRASALYASGAIKENVPEYVKTLADGAWIRNKTWLAKATLLTDDYAP
                     VDTLRNPL"
     gene            1762..2901
                     /locus_tag="VF531_09245"
     CDS             1762..2901
                     /locus_tag="VF531_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7714196.1"
                     /translation="MQNQPQATFDWDWSIRTADTILARRPVLAEKWNYEAGVILKALE
                     QVWRASGDPKYLEYIKKSVDHFVDEEGNIHTYRREDYNLDQVNSGKALLFLYRETGDS
                     RYARAARLIREQLRMQPRTGEGEFWHKQIYPHQVWLDGLYMATPFYAEYAALFNEPDR
                     FEDLAGQLFSVYHHTKDEKTGLLYHGWDESKSQSWANPVTGCSSCFWGRAMGWYTMAL
                     VDVLDYLPQTHPAREQLQENLGQIAAALIRVVDPSTGLWYQVLDQGERAGNYLEASAS
                     AMFSYAFLKGARLGYLGSDYKTRALASFEGMIRQFVRLDEQGWVHLDGICKVAGLGRY
                     SPEQPYRDGTFSYYIGEPVVSDDYKGLGPFILAGVEKERALAGDR"
     gene            complement(2913..>4138)
                     /locus_tag="VF531_09250"
     CDS             complement(2913..>4138)
                     /locus_tag="VF531_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEX7714197.1"
                     /translation="GAFTAAIRSAAACGGTVIVPAGEFLTGPITLESNLSLWLDAGSR
                     IVFSSNLADYPVIFSRWEGRDGNVYSPQIYGRGLENVTISGSGTLDGQGQSWWRLFRE
                     KRLDHPRPRLISLEDCSHILIEGIRLVNSPSWTIHPLFCRDLAITGVKIDNPADSPNT
                     DGIDPDSCQDVRIEECQIDVGDDCIAIKSGTEAASKKVASEKIVIAHCIMRHGHGGVV
                     MGSEMSGGISDVTVTDCTFYGTDRGIRIKSRRGRGGTIEDITARRITMEKVACPLAIN
                     LFYRCGPGGDDEIVRDKNPLPIGEKTPIVRGIILEEITAKNVGIAAGFCYGLPEQPVT
                     GLHLRDIRVELSESATPGLPEMMLDIEPISRKGFIGFNLKDVTFEQVRVSGQQGPAFE
                     ITQSDEVRYINSPEN"
BASE COUNT          918 a         1055 c         1152 g         1013 t
ORIGIN      
        1 gaaatggtgg gcagtcggct gcttgcccct accttcggat cctccattta tatctgggga
       61 tctttgattg tggtggtaat gactgcgctc actttgggtt attattacgg aggccggatc
      121 gccgacaaat tccccaatta ccgggtcatg ggactcgtcc tggcatgtgc cggggtttat
      181 atcgggttcc tgcctttttg ggcaaaatcc atgagtgatt cggtgatcac gctggaaccg
      241 aggcttggtt cgctcttggt atcgatggtc tttttcttcg tgccaagcgt cttgttggcg
      301 accatttcac cgttcggcat taagctatcg agccgcaacc tgacgaccat cggcaacacc
      361 gcgggcttca tgtcggcgat ctcaagcggc ggaagtattc ttggcaccct gttaacctct
      421 tttttcctca ttccggtcat gggcgttcgt aatattgtac ataccctggg aatcatccta
      481 ttagttctgg cactggtggt atttctggta gcccggcgac acaccgggag tgactccggc
      541 gaaaacggtc gggccgcctt gaaagggacc aacatcatga tctgccttgt tatactgggc
      601 ggtttgtcgt taagcggttt atgggttcta gcttcggtgc gggtgggtga ggagtggggt
      661 tataaaaccc tctacgagcg cgactccctc taccatcaca tccgggtggt tcaggacacc
      721 aactggcgtc ggttgcattt tgatgaatcc ttccaaagcg aaatggattt ggaaaacccc
      781 ttggaaatgg cctttcttta tacatcatac ctacatgtgg gggtaatcgc ccacccccaa
      841 ccgtcccgtg tgttattcat cggactgggc gggggctcgg cgcccaagaa gtttctcaat
      901 gattatccct cgttaaaggc tgtcgatgtg gtggagattg accccgaggt cgtgaaagtg
      961 gcgcgaaagt attttcaact gccggatgac cccaggttac gaatcttcgc ccaggatggc
     1021 cggatcttcg ttgaaaaaac ggcccgggag attgcggcgg gtaagacgga tccttatgac
     1081 atggtgatta tcgacgccta taccgccagt tccattccat accatttgac aaccagagaa
     1141 tttgtggccg cagttcgtaa aacgctctcc cgcgatggag tggtggtctc caacatcatc
     1201 ggcgccttcg ccgggcctgc aagcccgtta ctcagctcga tgactcgtac cattaagtcg
     1261 gttttccccc aaacctatct ttttccgacc tggaattggg aaggaaagaa tgactatgcg
     1321 gaaaacaatg tgattttggt ggccacggca gactctcggt attgggatgc cgcgacatgg
     1381 cgtaaacgcg ctagcgccct atatgcatcg ggagcgatca aggaaaatgt tccggaatat
     1441 gtaaagacat tggccgatgg cgcgtggatt cgtaacaaaa cctggctcgc caaggcgacg
     1501 ttgctcacgg acgattacgc ccctgtggat acgctgagga atccgttgta ataaacgttt
     1561 acccttggcg gttcattgtc gtgaaattgg aaccagtcag acggaaactg gccgagatag
     1621 cccacgacgc gattccatgc gcaagtaatg cgcattttta aaagtaaagg caatattcgc
     1681 acgaaatgtc ctgccgggtc acgctataat gaaagtggca gaaatccgca cccgaatcta
     1741 tttaagtgag gttatcaacc gatgcaaaat caaccacaag ccactttcga ttgggactgg
     1801 tcgatccgga ccgccgatac gatcctcgcc cgccggccgg ttctggcgga aaaatggaat
     1861 tatgaggcgg gagtgatctt gaaagcgtta gaacaggtct ggcgtgccag tggtgacccc
     1921 aagtatttag agtatattaa aaaaagcgtg gaccacttcg ttgatgagga gggcaatatc
     1981 cacacctatc ggcgagaaga ttataatttg gatcaagtga attccgggaa ggccttgctt
     2041 ttcttgtacc gggaaaccgg tgactcccga tatgcccggg cagcccggct catccgggaa
     2101 caattgcgga tgcagccccg taccggtgag ggagagtttt ggcataaaca gatctatcct
     2161 catcaggttt ggttggacgg tttgtatatg gcgactccgt tttacgcgga atatgcggcg
     2221 ctctttaatg aaccggacag atttgaggat ctggcaggcc aactattctc ggtatatcat
     2281 cataccaagg atgaaaagac cggtttgctc taccatggat gggatgagag caaaagccag
     2341 agttgggcca acccggtcac cggttgttcg tcttgtttct ggggacgtgc catgggctgg
     2401 tacactatgg ccctggtcga tgtattggat tatttgcctc aaacccaccc tgcccgggag
     2461 caactccagg aaaacctggg ccagattgct gcggcgctta tccgggtggt tgacccttct
     2521 accggtctct ggtatcaggt gctcgatcag ggggagcgcg ccgggaacta ccttgaagcc
     2581 tccgcatccg caatgttcag ctatgcgttt ttgaaaggag cacgcctggg gtatctcggt
     2641 tccgactata aaacccgggc actcgcaagt ttcgagggaa tgatccggca atttgtccgc
     2701 ctcgatgaac aggggtgggt gcatttggac ggaatctgca aagtggcggg tttgggaagg
     2761 tatagtcccg aacaacctta tcgggatgga acctttagtt attatatcgg cgaaccggtg
     2821 gtcagcgacg actacaaagg gctcggtccg tttatactgg ccggggttga aaaagaacgc
     2881 gctttggccg gagacagata agatacagcg cgctaattct ccggactgtt gatataacgg
     2941 acctcatcgg attgagtgat ctcaaaggcc ggtccttgtt gcccggatac ccggacttgc
     3001 tcaaacgtga catcttttag gttgaaacca ataaatcctt tacggcttat tggctcaata
     3061 tccagcatca tctcgggaag tcccggtgtg gcggattcgc tcaactcgac ccggatatca
     3121 cgcaagtgga gtccggtgac gggctgttcg ggaaggccgt agcaaaatcc ggcggcgatt
     3181 cctacattct tggcggtaat ctcctcaaga atgattcccc ttactatcgg tgttttttcg
     3241 ccgatgggga gggggttctt gtcacggacg atctcgtcat ctcctcccgg gccgcatctg
     3301 taaaaaaggt tgatcgccaa aggacaggcc actttctcca tggtgatccg gcgggccgtg
     3361 atgtcctcga tggttccgcc ccgtccccga cgggatttaa tccggatgcc gcgatctgtt
     3421 ccgtaaaatg tgcagtccgt tacggttaca tcgctgatcc cgccgctcat ctcgctgccc
     3481 atcaccacgc cgccgtgacc gtgtctcatg atgcagtgcg cgatgacgat cttttcggag
     3541 gctacttttt tactggcagc ctcggtccct gacttgatgg cgatacagtc gtctccgacg
     3601 tcgatttggc attcttcgat ccgcacgtct tgacaggaat cggggtcaat tccgtcggta
     3661 tttggcgaat cggcagggtt gtcgatcttt accccggtga tggctagatc tctacaaaac
     3721 agcggatgaa tcgtccagga cggggaattg accagccgga ttccttcaat taatatatga
     3781 gaacagtcct caaggctgat cagacggggg cgggggtggt ccagtcgctt ctcacgaaac
     3841 aggcgccacc aagattgacc ttgtccgtct aaggttccgc ttccgctgat ggtgacgttc
     3901 tccagacccc ggccatagat ctggggcgag tatacattcc cgtccctgcc ttcccagcgg
     3961 gaaaagatga ccggatagtc cgcaagattg gatgagaaaa caatccggga tccggcgtcg
     4021 agccataaag atagattgct ctcaagggta atcgggccgg tcaaaaattc accggccgga
     4081 acgattaccg taccgccgca ggcggctgcg gaccgaatgg cggcggtaaa ggccccgg
//
LOCUS       DASVMB010000325         6181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_40386,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000325 DASVMB010000000
VERSION     DASVMB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40386"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF531_09255"
     CDS             <1..332
                     /locus_tag="VF531_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714198.1"
                     /translation="VLIRKWQMGMRLIGYTQWAKVLLAILVAIPLLYKKPPTVLARLI
                     KNHPGIMRGILGLTFTALIALVVNDSGIVAVATMFIFGAFMLLQVIFKEWVYKDEPCA
                     SERRFTQ"
     gene            329..1948
                     /locus_tag="VF531_09260"
     CDS             329..1948
                     /locus_tag="VF531_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX7714199.1"
                     /translation="MSSGIQRIPIGPELRIEQLVKLMVNSTEKQLVLEVPLQTEPGDT
                     PDGTPVGAALFHDEINLRLLKFYAEEAEKDFVIATTDPVVAALAKRLGIPTWKEDESR
                     KILAEKDSDETVQLSGGINAAVRRSADEMAAAPESATVPVVNSTGISRGERQSGRGFI
                     PAVLIALFTITLAVWWFFQPKAIVTVYPKVTDRQFLVTARTSSLFRDDQTLTGKLPAN
                     ILEKTDSIQVQTVATGKKNIGVTPATGKVLFTNSSNQPVVLPKGSILMGRAGVRYLTD
                     GPLLVPAKKVQYDSGIEAGVTYGRAEVTVTAAVKGVIGNQPAKSITLLEGKFGRVLKV
                     TNPTPTKNGADKQISVVTLEDTKKGDAEAREQMGLLVPDAVSGLVGQDYLYLPELVKN
                     EVLQITYGPDIGAEADTVQTNLEYRIAVLAPYRVEINKLLNHELERLLPAGFQSTGRP
                     VETVSAKVISSGTDWADLELSARGQIRGVLDQDKIKKLIGGQSLGKAKTILLGEEEIA
                     TSQINLKTGGDKLPRFPFQIKVIFPAGARAR"
     gene            1948..2892
                     /locus_tag="VF531_09265"
     CDS             1948..2892
                     /locus_tag="VF531_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7714200.1"
                     /translation="METNPLGYTGIMVSRLCFGTLALSPIQKECPSEQAAELFLRAYD
                     LGVNFFDTAELYQNYEPLAIALQSRPQMVISSRSYAVTFAEMRRSIDLARTALNRDWI
                     DIFGIHEIESEATLRGHRGALDYLCQAKAQGIIRAVAISTHTVAGVRAGATEPAVDVI
                     HPLINREGIGIRDGSVAEMIAALQTAKEFGKGIYAMKVLAGGHLNGDAPAAIRFITGL
                     DLADSMAIGMQSLAEVRLNLELISGTTPDKALLAEVGRTKRFLRIADWCHGCGRCVSR
                     CGFGALSLVNGRVAVDQARCMHCGYCARVCPDFCLKIM"
     gene            2907..3320
                     /gene="ruvX"
                     /locus_tag="VF531_09270"
     CDS             2907..3320
                     /gene="ruvX"
                     /locus_tag="VF531_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7714201.1"
                     /translation="MRWMGLDCGERRIGIAFSDPLELTAQAYAFRERSASLRKDLEFF
                     HELIHTYGVEGVVLGLPKNMNGSEGPMAEKVRNFGKTLEENEGLTVVYWDERLSTISA
                     HRVLLEADLSRRKRRVKVDQLAAAIILQNFLDSRR"
     gene            3361..3660
                     /locus_tag="VF531_09275"
     CDS             3361..3660
                     /locus_tag="VF531_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEX7714202.1"
                     /translation="MTEQENSWVTLVDEEGHEHRFNLLNIVEMDGVKYAVMVPEVQPS
                     EEEEEAVIFRLETDEAGEEVLVDIEDDDEFAKVCELLDTMNEEAEEEETDQEPEK"
     gene            3786..4928
                     /gene="mltG"
                     /locus_tag="VF531_09280"
     CDS             3786..4928
                     /gene="mltG"
                     /locus_tag="VF531_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7714203.1"
                     /translation="MLKLTSWFENIRNMCRTAIKGLTSFCKRICAYVPDFLKNHPRLG
                     GGIIISFVFIVILGIGFFWNMNPPQFGAKQAGIFKVRYGSSLREVARALKSEGYIRNR
                     FVYEWYVRIHPKGRMAKAGWYRLSPGMSVSKITKVLQRGTPQEIRLTIPEGLTVKEVA
                     ALLGEKGLVNPESFLARLKDRQLITGILGDMAPATLGDNFPEGYLFPDTYNLVLPVTD
                     QEIITVMLNRFKEVFSRNFGKLSPARKRQILIVASLVEMEAKHDPERPVIAAVFYNRL
                     RLGYPLQSCATVQYALGTRKKRLYDKDLQVNSTYNTYLHYGLPPGPIANPGLASLKAA
                     ANPAQVSYLYFVAKPDGNHIFSRTYREHLNAQHGIESESGRRISLQ"
     gene            4979..5752
                     /locus_tag="VF531_09285"
     CDS             4979..5752
                     /locus_tag="VF531_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7714204.1"
                     /translation="MRITLLGTGTSHGVPVLGCECPTCTSTDPRDFRTRSSAYVCMGE
                     NGPGILIDTATEFRLQSLKNRVSRVDLVLMTHCHADHVCGFDDLRRFNELQNQTIPVY
                     GNHDTIVQIARMFSYIFDPRAQVGGGKPRIDLHEVSGEFTANGVRITPIPLRHGAIPV
                     LGYRIGGFAYITDCSEISQASLSLLENLDLLVLGVLRFRPHPTHLNLEAGLELIRRLK
                     PRRCLFTHICHDFLHRELSGVLPEGIRPGYDGEQIDIER"
     gene            5755..>6181
                     /locus_tag="VF531_09290"
     CDS             5755..>6181
                     /locus_tag="VF531_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX7714205.1"
                     /translation="MEDSLIIGTTAVDTVRIYAASTTGLVEEARRIHQTWATASAALG
                     RVLTGTIILGVMADNLRRLTVEVCGDGPLGKLMAVSNQRGTVKGYLDNPQVDLDLNSM
                     GKFDVGKAVGKGELVVTKELGIGEPYQGIVPLQTGEIAED"
BASE COUNT         1482 a         1470 c         1735 g         1494 t
ORIGIN      
        1 ccgtattgat tcgtaaatgg caaatgggta tgcgtctcat cggttatact caatgggcca
       61 aggtgttatt ggcaatatta gtagccatcc ccttgctgta taaaaaacca cctacggttc
      121 tagcacggtt aattaaaaac catcccggca ttatgcgggg aattctgggt ttgacattca
      181 cggctctgat agcactggtg gttaacgact caggaatagt ggccgtggca accatgttta
      241 tctttggggc attcatgctg ttacaggtga tctttaagga atgggtgtat aaagatgagc
      301 cgtgcgcgtc cgaaaggaga ttcacgcaat gagtagcgga attcaaagga ttcctatcgg
      361 ccccgaatta cgaatcgaac aattggtcaa actgatggtc aacagtaccg agaagcaact
      421 ggttctggag gtaccgctcc aaactgaacc gggggataca cccgatggaa ccccggtcgg
      481 agcggcgctg ttccatgatg aaatcaactt gcgattattg aaattttatg ccgaggaagc
      541 tgagaaagat ttcgtcatcg ccaccaccga tccggttgtg gcggctctgg caaaacgact
      601 gggaattccg acatggaagg aagatgagag tcgtaagata cttgcggaaa aggactccga
      661 tgaaacggta cagctcagcg ggggcataaa tgccgcagtc cgccgcagcg cggatgagat
      721 ggccgccgct cccgagtccg ccactgttcc ggttgtcaat tccaccggga tttcccgggg
      781 agagagacag tccggacggg gctttattcc ggcggtccta atcgcgcttt ttacgatcac
      841 cttggcggtt tggtggtttt tccagcctaa ggcgattgtc actgtttacc caaaggtcac
      901 cgacaggcaa tttctagtca ccgcccgaac cagtagcctt ttccgggatg accagacgct
      961 caccggtaag cttcccgcaa atattctgga gaaaaccgat tcgattcaag ttcagaccgt
     1021 ggcaacagga aagaaaaata tcggtgtcac accagccaca ggtaaggtac ttttcaccaa
     1081 tagcagcaat cagccggtgg tgctgcccaa gggtagcata ctcatgggtc gcgccggggt
     1141 tcgttatctc accgatgggc cgctcttagt acccgcaaaa aaggtgcagt acgattcggg
     1201 gatcgaggcg ggggttacat acggccgggc tgaggtcaca gttactgcgg cagtcaaagg
     1261 agtcatcggc aatcaacccg ccaaatccat cacccttctt gagggaaaat tcggacgggt
     1321 tcttaaagtg acaaatccca ctcccacaaa gaatggggca gataaacaga tcagtgtggt
     1381 gacactggag gataccaaaa agggtgatgc cgaagccaga gagcaaatgg ggttactggt
     1441 tccggacgcc gtatccgggc tggttggcca ggattatctg tatcttccgg aacttgtgaa
     1501 aaatgaagtc ttgcagatta cgtatggtcc ggatatcggg gcagaggctg atactgttca
     1561 aaccaatttg gaataccgta tcgcggtgct tgctccctac cgggtggaaa tcaataagct
     1621 tctcaatcac gaattggagc ggctgctccc cgcgggattc caatccaccg gccggccggt
     1681 ggagacggtc tccgccaagg tgatttcttc cggtacggac tgggccgacc tggaactgtc
     1741 ggctcgggga cagatccggg gagtgcttga tcaggacaag atcaagaagt taatcggcgg
     1801 tcaatcgctc gggaaggcaa aaaccatcct gctgggcgaa gaggagatcg ccacctccca
     1861 gatcaacttg aaaaccggag gggacaaact gccgcggttc ccatttcaaa tcaaggtgat
     1921 ttttccagcc ggagcccgtg ccaggtaatg gagacgaatc ctttgggata taccggtatc
     1981 atggtatccc gactctgctt cggcactctc gcgcttagcc ccattcaaaa ggagtgccct
     2041 tcggagcaag cggcggagtt attcctccgc gcctacgatc ttggggtcaa tttcttcgac
     2101 acagcggaac tctatcagaa ctacgagcca ttagccatag cacttcaaag ccgtcctcag
     2161 atggtcattt ccagccgctc gtacgcggtg accttcgccg agatgcgccg gagtatagat
     2221 cttgcccgga ccgcccttaa ccgggactgg atcgacatct tcggaattca tgagatagag
     2281 agtgaggcaa ctcttcgggg acaccggggg gctctggact acctctgtca ggcgaaagcg
     2341 cagggaatca ttagagcggt agcaatctcc actcatactg tagctggagt acgggccggg
     2401 gcaaccgaac cggcggttga tgtaatccac ccgttgatca accgggaggg tatcgggatc
     2461 cgcgacggtt cggtggctga gatgattgcc gcccttcaaa ctgctaaaga attcggtaag
     2521 ggaatctacg cgatgaaagt tctggccggc ggtcacctaa acggtgatgc cccagccgcg
     2581 atccgtttca ttaccggact tgatttggcg gactccatgg ccatcggcat gcaatccttg
     2641 gccgaagtca ggctcaattt ggaattgata tccggaacca cccctgacaa ggcgctcctt
     2701 gcggaagtgg gtcgaaccaa acgttttctg cggatcgccg attggtgtca cggttgcggc
     2761 cgttgtgtgt cacggtgtgg ctttggcgcg ctcagcctgg taaacggccg ggttgccgtg
     2821 gatcaagccc ggtgtatgca ttgcggttac tgtgccaggg tttgcccgga tttttgttta
     2881 aaaattatgt aggggcgggg gttgcaatgc ggtggatggg tttggactgc ggtgaacgcc
     2941 ggatcgggat cgctttcagc gacccgttgg agcttacggc tcaggcatat gcctttcggg
     3001 aacgctccgc ttccttgcga aaggatctgg aatttttcca tgaactgata catacttatg
     3061 gagtcgaagg cgtggtcctg ggattgccta agaatatgaa cggcagcgag ggtccgatgg
     3121 ccgaaaaggt acggaatttc ggtaaaaccc ttgaggaaaa cgagggtttg acagtcgttt
     3181 attgggatga acggctctcg accatctcag cccatcgggt gctgcttgag gcggatcttt
     3241 cccgtcgcaa gcgccgtgtt aaggttgatc agctggctgc ggcgattatt ttacagaatt
     3301 ttttagattc ccgccgctga taatataaag agataggaaa atttcgagga ggtcaatcaa
     3361 atgacggaac aagaaaacag ctgggtcaca ctggtagatg aagaaggaca tgaacaccgg
     3421 tttaatctgt tgaacatcgt ggagatggat ggcgtgaagt atgcggtaat ggtcccggaa
     3481 gtccagccca gcgaggaaga ggaggaagcg gtgattttcc gtttggagac cgacgaggcc
     3541 ggtgaggagg tcttggtgga tattgaggat gatgacgagt ttgccaaggt ttgcgaactt
     3601 ctggatacaa tgaacgagga agcggaagaa gaggagacgg accaggaacc ggagaagtaa
     3661 taagtgagac attaattttc aaccctgatt aacccgcaac aataactgat ccgaataact
     3721 aataactgtt tatttgtcaa gacggctgtt ttttagtata ataaaaccag gaggatgagc
     3781 aaagcgtgct gaaactaacc tcttggtttg agaatatccg taatatgtgc cgtacggcta
     3841 tcaaagggtt aacctccttt tgtaaacgta tttgcgccta tgtaccggat tttttgaaaa
     3901 accaccctcg gctcgggggt ggaattatca tttcatttgt ctttatcgtc atcctgggaa
     3961 taggtttctt ttggaatatg aatccgcctc aatttggcgc gaaacaagcc gggattttta
     4021 aggttcgtta cggctcttcc ttgcgggaag tggcccgggc tttaaagtcg gaaggttata
     4081 tccgtaaccg gttcgtttac gaatggtacg tgcggattca ccccaaaggc cggatggcca
     4141 aggccgggtg gtatcgttta agtccgggaa tgtctgtttc caaaatcaca aaagttttgc
     4201 aacgcggcac cccccaggaa attcggctga ccattcccga agggttaaca gtaaaagagg
     4261 tcgcggcgct cctgggtgaa aaaggattgg tcaacccgga gagttttttg gctcgtttga
     4321 aagatcggca acttatcacc ggtattttgg gagatatggc tccggcgaca ctcggcgata
     4381 attttcccga gggctacttg tttccggata cctataattt agtcctccct gtcactgacc
     4441 aggagatcat cacagtgatg ttgaaccggt ttaaggaagt tttcagccgg aatttcggga
     4501 aactttcccc ggccaggaaa cgccagattt tgatcgtcgc ctccctggtg gagatggagg
     4561 ccaagcacga tccggaacgc ccggtaatcg cggccgtatt ttataaccgg ttacgtttgg
     4621 gctacccctt gcagtcctgc gccaccgtac agtatgcttt gggaacccgg aaaaagcggt
     4681 tatacgataa ggatttacag gtcaactcca cctataatac ttatcttcat tacggtttgc
     4741 cgccgggacc catcgccaat cccggtctgg cttctttaaa ggcggctgcc aatccagcgc
     4801 aggttagtta tttatatttc gtggccaagc ctgatggtaa ccatattttt agtcggacct
     4861 accgcgagca tctcaacgct cagcacggga tcgagagcga gagcggccgg agaatctcct
     4921 tacagtaaag attcttttat tttgtcgcct ggcatcccca tagatcatga ggaattatat
     4981 gcgaattact ttgcttggaa ccggaacctc tcatggagtt ccggttttag gttgtgaatg
     5041 cccgacttgc acttctacgg atccccggga ttttcgtact cgctcctcgg cttatgtgtg
     5101 tatgggtgag aacggtccgg gcatattaat cgataccgcc accgagtttc gcttgcagtc
     5161 tttgaaaaac cgggtctcgc gggtggatct ggtgcttatg actcattgcc atgcggatca
     5221 tgtgtgcgga tttgatgatc tacgacgttt caatgaactt caaaaccaaa caatcccggt
     5281 atacggcaat catgatacca tcgtccaaat tgcccggatg ttctcctata tttttgatcc
     5341 ccgggctcaa gtggggggag gtaaacctcg aatcgacctc catgaggtga gcggggagtt
     5401 taccgctaac ggggtccgta ttactcccat accattacgg cacggagcca taccggtact
     5461 gggttatcgg atcgggggtt tcgcttatat taccgattgc agtgagatat cgcaagccag
     5521 cttgtccttg cttgagaatt tggatctgtt ggttttggga gttttacgat tccgccccca
     5581 tcccacccat ctgaacctcg aagccgggtt ggaattgatc cggcgattaa aaccgcggcg
     5641 atgtcttttt actcatattt gccacgattt ccttcatcgc gagctctccg gcgtgcttcc
     5701 ggagggaata aggccgggct atgacggaga acaaatcgat attgagaggt aatcatggaa
     5761 gactctttaa tcattggaac gacggctgtg gatacagttc ggatttacgc cgccagcacc
     5821 accggtttgg tggaggaggc ccgaaggatt catcaaacct gggcaaccgc cagcgccgct
     5881 ttgggacgtg ttctgaccgg gactatcatc ttgggggtca tggccgataa tttacgccgt
     5941 ttgaccgttg aagtctgcgg tgatgggccc ctggggaagc tgatggctgt atccaatcag
     6001 cgcggcacgg tcaaaggata tttggacaat ccccaggtgg acttggattt aaactccatg
     6061 gggaagttcg atgtcgggaa ggcagtcggt aagggggaat tggtcgtaac caaagagctc
     6121 ggaatcgggg agccttatca gggaatcgtg ccgcttcaaa ccggggagat tgctgaagac
     6181 t
//
LOCUS       DASVMB010000326         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_40436,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000326 DASVMB010000000
VERSION     DASVMB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40436"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="rplU"
                     /locus_tag="VF531_09295"
     CDS             complement(<1..351)
                     /gene="rplU"
                     /locus_tag="VF531_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014016435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7714206.1"
                     /translation="MYAVIECGGKQYRVQEGERVRVEKIKAGIGENVTIDKVLLLGGE
                     KTVVGTPFVEGAAVTCKVLNQDKDAKVLVFHYKAKKNIRKRYGHRQPFTDLLVENIVG
                     SGFTGTPKTVKTVKT"
     gene            complement(552..2237)
                     /locus_tag="VF531_09300"
     CDS             complement(552..2237)
                     /locus_tag="VF531_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7714207.1"
                     /translation="MTKEILVNIGIDEVRVAMLEDGGLAEFSVERSDEQRRAGNIYRG
                     RVKNVLPGMQAAFIDIGEEKNAFLYVDDIVSRNRENGGAPPAQKPSIRELLHEGQELI
                     VQMIKEPIGTKGPRVVTQITIPGRYLVLVPGEDYIGVSRRIDSEAERDRLKAVVSKFK
                     PPEVGLIVRTAAAEAETGDLQSEYEFLINVWKKLQRKTKKGPCPALLYRDHDLLYRVL
                     RDYLSKDINRILIDELDAYTKAQELIKSLAPSLKNRIQLYSDEQPLFERFNVEQQLAK
                     ALKRKVWLDCGAYLVFDQAEALIVIDVNTGKFTGSTSLEDTVFQTNIMAVEEIARQIR
                     LRNLAGIIIIDFIDMVSDAERAKVIEKLKAEFERDKIKANILGFTSLGLLELTRKKVK
                     QSLREILQTECEYCDGTGYHASMENYAYQAIRTMQQLAQRNAAEALLLAVNPRVGSIL
                     IGPGGANLEKMERTLNKTIFIKGQECLSFDQVHILACGTKMEIESLALPVREGAELDL
                     KVTETHIANPGDGIGRIEGYIINVEGAGNRVGRTVRVLVTKTFRTYAKAKIIG"
     gene            complement(2234..>2839)
                     /locus_tag="VF531_09305"
     CDS             complement(2234..>2839)
                     /locus_tag="VF531_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX7714208.1"
                     /translation="SEGYHPHPKLSFGPALALGIASTSEYFDVGLGREMEPASIQNSL
                     NQVLPESLRITAAGRLNEPVIPLNALINRAAYRIELTMVPQSLPGVKADLAAIISKQE
                     LVVSRNTKTGQKMVNIRPLLHNLTMVETSEDPGDRAHVLFVFDISGEIGNNGNLRPDE
                     ITGLIKQPLTVSGITRIGLWREERGMIIKPMDLCVQRPKIQ"
BASE COUNT          645 a          768 c          671 g          755 t
ORIGIN      
        1 agtcttcacg gttttgacgg ttttcggagt accggtgaaa ccggatccca cgatattctc
       61 taccaaaaga tcggtaaaag gctgccggtg gccgtaacgt ttccggatat tctttttggc
      121 cttgtaatga aaaaccaaaa ctttggcgtc tttatcctga ttcagtactt tgcaggtcac
      181 cgcggcgcct tcaacgaacg gagtgccgac aacggttttt tcgccgccca acagcaacac
      241 tttgtcaatg gtgacgtttt caccgattcc cgctttgatc ttctcgaccc ttacgcgttc
      301 accttcctga acccggtatt gtttcccacc gcattcaatt acagcataca tttgtttaca
      361 cctcctcgca ctagactcgc cgtttcggta ctccggcctt aacggttccg gagatttaaa
      421 accttcccga gcggttgcgt gtcctggtaa accacgggca cggtgggatg caagcgcgta
      481 cgccatttga cgacgcacat ctcaaaaacc cacgaagata atattaccat gttccgaagc
      541 ggaagtcaac gttaaccgat gattttcgct ttggcgtaag tcctgaaagt cttggtgacg
      601 aggacccgaa ccgtccggcc tacccggttt ccggcgccct ccacattgat gatgtaccct
      661 tcgatccggc cgatcccgtc accaggattg gcgatatggg tttcagttac tttcagatcg
      721 agttcggcgc cctcgcgtac cggcaaggcc agactctcta tttccatttt ggtcccgcaa
      781 gcgaggatat ggacctgatc aaagcttaaa cactcttgac cctttatgaa tatggtttta
      841 ttaagggtcc gctccatttt ttccaggttg gcaccgccgg gtccgattaa gatcgaaccg
      901 acccggggat tcaccgccag cagcaaggcc tcagccgcat ttcgttgcgc cagttgttgc
      961 atggtacgta ttgcctgata ggcatagttt tccattgagg catgataccc ggtgccatca
     1021 cagtactcgc attcggtttg caaaatttct cttaaactct gtttaacctt tttacgggtt
     1081 aattccaaaa gccccaacga agtaaagccc agaatattcg ctttaatctt atcccgttca
     1141 aactcagcct tgagtttctc gatgactttc gcccgctcgg cgtcggaaac catatcgata
     1201 aagtcaatga tgatgatccc tgccagattg cgcagccgga tctgacgagc gatctcctcc
     1261 accgccatga tattggtttg aaagaccgta tcttcaagag aggtggaacc ggtaaactta
     1321 ccggtgttga catcgatcac aatcagcgcc tcggcctgat cgaaaaccaa gtaagctccg
     1381 caatccagcc aaaccttgcg tttcagagcc ttggccagtt gttgttcgac attgaatctt
     1441 tcgaaaagcg gttgttcgtc gctatagagt tgtatccggt ttttaaggga gggcgccagg
     1501 cttttgatga gctcctgggc tttggtataa gcatccaact catcaataag gatcctgttt
     1561 atatccttgc tcaggtaatc ccggagtact cgatagagga gatcgtgatc ccggtaaagc
     1621 aaagccggac atggaccctt tttggtcttg cgttgcagct ttttccagac attgatcagg
     1681 aactcatact cgctttggag atcaccggtc tcggcttcag cggcggcggt ccggacaatc
     1741 aacccaactt ccggaggttt aaatttggag acaactgcct tcagtcggtc ccgttcagcc
     1801 tcgctatcaa tcctgcgtga tactccgata taatcttcac cgggaaccag taccagatac
     1861 cggccgggaa tggtaatctg ggttactacg cgaggtccct tggtgcctat cggttcctta
     1921 atcatttgga cgataagttc ttgtccttca tggagcagtt cccggatgga cggcttctgg
     1981 gcgggaggcg cacccccgtt ttcacggttt cgggagacaa tatcgtccac atagagaaag
     2041 gcgtttttct cctccccgat gtcaataaag gcagcctgca tccccggaag gacatttttt
     2101 acccttcccc ggtaaatgtt ccccgcccgg cgctgctcat cggagcgttc caccgaaaat
     2161 tcggccaggc ctccatcctc gagcattgcc acgcgaacct catcgattcc gatatttacc
     2221 aaaatttcct tggtcattga attttcggcc tttgcacaca taaatccatc ggtttaataa
     2281 tcattccccg ttcctcgcgc cataagccaa tccgggtgat acccgacacc gtcaggggtt
     2341 gtttgatgag cccggtgatc tcatcgggtc tgagattgcc gttgttacca atctcgccgc
     2401 tgatgtcgaa gacaaacaaa acatgggctc tatcacccgg atcctcagaa gtctctacca
     2461 tagttaagtt atgcaacaat ggccgaatat tgaccatttt ttggccggtc ttggtgttcc
     2521 gggagacgac aagttcttgt ttagagatga tggcggccaa atcggccttg accccgggca
     2581 gtgattgagg aaccattgtc aattcaattc ggtatgcggc gcgattaatc agtgcgttta
     2641 gcggtatcac cggttcgttt aggcggccgg ccgcagtaat ccgtagagat tcgggtaaaa
     2701 cttggttcaa agagttctga atcgaagcag gctccatttc ccgcccgagc cccacatcga
     2761 aatattcact ggtacttgcg atgcctaacg ccaaggccgg accaaaacta agtttggggt
     2821 ggggatgata accttctga
//
LOCUS       DASVMB010000327         6969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_263373,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000327 DASVMB010000000
VERSION     DASVMB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263373"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF531_09310"
     CDS             complement(<1..560)
                     /locus_tag="VF531_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714209.1"
                     /translation="MIKILGTVEKWIQNSFFSRWKVFFQILTIILVMVFFMGFQGYNS
                     IRIINSMQSQVQKIFNENVKYMNDISNIRADIKTVQQHYFESIHGIQSVTSVKRDIDQ
                     LDEKIQMLSSVDLKTCRDLQTELKKVRQIVENGASDAGYDQLKQSATMMDLQIGALNS
                     QLLSKGIQSMAGNNAFSRASRTDTIFA"
     gene            complement(665..1882)
                     /locus_tag="VF531_09315"
     CDS             complement(665..1882)
                     /locus_tag="VF531_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7714210.1"
                     /translation="MHPLELLVPAGNLEKLRTAFLYGADAVYIGAEGLSLRAGQAEFG
                     PEEIKTGVDEAHRLGKRVYTAVNIFAQNRDLALVAEQLGLLASIGCDGVIVSDPGVIR
                     IAQRVAPDLPLHLSTQANTTNVESVRFWRDQGVKRIIPARELSIAEIAAIAAAVPEIE
                     LELFVHGAMCLAYSGRCLLSAYRTGRSSNRGDCTHPCRWEHLLVESTRPYDPLVLEED
                     NRYSYILSSKDLCLMDRLPEMLGAGVKSCKIEGRMKSVYYIAVVTRTYRWALETLFKD
                     PATYQTRPEWFAELSKISNRGYTHGFFDGVPENNMQNPEVKYFQSHDLAGTVLSYERE
                     GRVLVGVRNRITREDSLELLLPETTIRLDSKQMVDEHGLPLDQAHNGYRVYLPVSEPV
                     PAGTVIRKQLLES"
     gene            complement(2066..4147)
                     /locus_tag="VF531_09320"
     CDS             complement(2066..4147)
                     /locus_tag="VF531_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7714211.1"
                     /translation="MPETKFTNRLIYETSPYLLQHAHNPVDWFPWGDDAFQKAKAENK
                     PVLLSCGYSACHWCHVMERESFEDPEIAALMNKYFVNIKVDREERPDIDQLYQQAVAA
                     ITGQGGWPLTVFIDHQGRPFFGGTYFPPTASYGRMTFPELIEAVHQKWLSEQERIAEA
                     GQELVEYLRAEPGSSPETGIPGPELPLRAIGELGGEVDRQNGGFGRAPKFPNTPVLGL
                     FLRVGTAAGQKEAVDLALFSLRRMARGGIYDQLGGGFHRYATDAIWLVPHFEKMLYDN
                     AQLLQIYTNAFQITQEAEFGEVVRDTAAYIRREMTAPEGGFYATQDADSPEGEGRFFL
                     WSIAEVQKILDPETAKLFINHFRMTEAGNFEGLNILNRLNETANPSKEWEYNRKLQQA
                     RAALLAVRETRPRPFRDEKIITSWNGLMIGALAVVYQVFGNPADYRAAQKAAGFILDK
                     MRPAAGRLVRVYKDGQAKGAAFLDDYAFLVRGLLNLYETDFRPLWLAESLELTKTVLR
                     KFGDGSGKYYLTADEPGLIARPLSGADQAIPGGVAVHCENLLRLAEFTGKTELRDAAA
                     EILTSYGESAKRNVWGYASLIRALDMYHRSFKEFVFVSETPALPEILVKLRKLYLPYR
                     ILARQVQNDGDSSETQADHPARDLFTGRTPVNGKPTCYICHDQHCLPPVTEWEDLCGI
                     LEQLQHQENRQ"
     gene            complement(4241..5338)
                     /locus_tag="VF531_09325"
     CDS             complement(4241..5338)
                     /locus_tag="VF531_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714212.1"
                     /translation="MMTKRKQIVEVLNAFDPLFEDFYTWLAGQYDPSFGGFYYAVSSK
                     KSPEFQPDIESTGQAVHILGNSNLLQQMPEEMKNQVISFFQKRQTPEGYFFDFQNDMR
                     NFERMVGRAIMYSCTALNMLGGHPLYSLPGSGENRDTLPDCLKSLAALKKWIDERPWH
                     YAWMAGDNIQAIGVYLRAIPEAEAEKYIEFICNYLLEHQDPQTGLWGEGRPYVRISGA
                     FKLALFYRDIGKPLPRAELIYQSLLDTLHNDISEDMCWTRNPIDLLRVLKRQLGDYAE
                     DDILDILTITYKNLEQYLKPDGGFSRHVKWSLEMANNVRLGKGLAEGDMNASTQALRI
                     RNTVYEISGQMEKMNHLEQYARDFYDRIILK"
     gene            5664..6656
                     /locus_tag="VF531_09330"
     CDS             5664..6656
                     /locus_tag="VF531_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7714213.1"
                     /translation="MKNIVILATGGTIAGVSSSITDTANYQPAILPIEAITKDIAEPI
                     RKVANISSEQIAQIDSVEMTHQIWFTLANRINALLASPQVDGAVVTHGTDTLEETAYF
                     LNLVVKSDKPVVVVGAMRPASALSSDGPMNLYNAIILAASDEATGKGVLVTLNNSINS
                     SREVTKTNTSLQDSFKAPELGYIGYLIEGEPHFYREPTRKHTYMSEFIIDDLTCLPPV
                     EIIYGHVGNMSALAQAAVAAGAKGLVYAGMGNGNMSKMMKNTFMDIQEHGVVVVRSTR
                     VSSGIVTRYGADNDDLSNFIVSDTLNPQKSSILLSLALTRTTDPAEIQRMFWQY"
BASE COUNT         1774 a         1812 c         1549 g         1834 t
ORIGIN      
        1 gcaaaaatag tgtccgtcct ggaggcccgg gaaaatgcat tgttgcccgc catggattga
       61 atgcccttgg aaaggagttg actattcaaa gccccaatct gcaaatccat catggtggcg
      121 gactgcttga gttgatcata accggcatca ctggcgccgt tttctacaat ctggcgtacc
      181 tttttgagtt ccgtttggag atctcgacat gtcttcaaat cgaccgagct aagcatttga
      241 attttctcat ctagctggtc aatatcccgt ttgacgctag tcaccgattg tataccgtga
      301 atactctcaa aataatgttg ctgaactgtt ttgatatcag cacgaatgtt actgatatcg
      361 ttcatatatt ttacattttc attgaaaatc ttctgcacct gggattgcat gctattaata
      421 attcgaatgc tattataacc ctgaaacccc ataaaaaaaa ccatcaccag aatgatagtc
      481 aaaatttgaa aaaaaacttt ccaccgtgag aaaaaagaat tttgaatcca tttctccacg
      541 gttccaagta tcttgatcat caatgtcacc tcgattgatt agtactatta ttttcggtaa
      601 agtagcagta tactttagaa ttgtttttcc aaccattctc atcttcatga gaaaagacaa
      661 tctgttacga ttcaaggagc tgtttccgga tgactgtccc ggccggaacc ggttcggaaa
      721 ccggaaggta aacccggtac ccgttatggg cttggtcaag cggcaggccg tgttcatcga
      781 ccatttgctt ggaatccagc cggatggtgg tctccggcaa taacagctca agcgaatcct
      841 cccgggtgat ccggttccgt acccctacca gaacccgtcc ctccctttca taggatagta
      901 ccgttccggc cagatcgtgg ctttgaaagt atttgacctc cggattttgc atattgtttt
      961 cgggaacacc gtcaaagaac ccgtgagtat atccgcgatt cgaaatctta ctcaattccg
     1021 cgaaccactc ggggcgggtt tggtacgttg caggatcttt aaacaacgtt tccaaagccc
     1081 aacggtaagt ccgggttacc acagctatgt aataaacgga tttcattctg ccttcaatct
     1141 tgcaactttt gacacccgcc cctaacattt ctggaagtcg gtccatcaga caaaggtcct
     1201 tggaacttaa tatgtaactg taacgattat cttcctccaa aacaagcgga tcatacggcc
     1261 gggtcgactc aaccagcagg tgttcccaac ggcacgggtg agtgcaatcc ccccggttgg
     1321 aactgcgacc ggtccggtac gccgatagca aacaacggcc cgaataagca aggcacatcg
     1381 caccgtgcac aaagagttcc agctcgattt cgggtactgc cgcagctatg gcggcgattt
     1441 cggcaatact cagttcccgg gcgggaatga tccgctttac accctgatct ctccagaacc
     1501 ggacggattc aacattggtg gtgttcgcct gagtactcaa atgaagcggt aaatccgggg
     1561 ccaccctttg ggcaatccgg atgactccgg gatcactcac gatcaccccg tcgcaaccaa
     1621 tactcgccaa aagccccaac tgctccgcga ctaacgccag atcacggttt tgcgcgaaaa
     1681 tattcaccgc tgtgtaaacc cgttttccca accggtgggc ttcatcaacc ccggttttta
     1741 tttcttccgg tccgaactca gcttgtccgg cccggaggct cagtccctct gctccgatgt
     1801 agacagcgtc cgctccatat aaaaaagcgg tccgtaattt ctccagattc ccggcaggga
     1861 ctaaaagttc tagtggatgc atccaaccct ccgcactaat tgatagcctt attttaccaa
     1921 ggcaacaggg atcaggcaac aaaatgtagg tatttcgatg aagtaaatca atgaatcagc
     1981 cgggtcaagt ctccttccgc ttgacttact catacctgtt gccttttccc tattgcctgt
     2041 tgcccgcgcc cgcagggtgc gatgcctatt gcctgttctc ttggtgttgc agctgttcca
     2101 ggatcccgca aagatcttcc cattccgtta ccggcggcag acaatgctga tcgtggcata
     2161 tataacaagt gggtttgccg ttaacaggcg tccgcccggt aaacaaatct cgagcgggat
     2221 gatcggcctg tgtctcggat gaatccccat cgttttgcac ttgacgggcc aaaatccgat
     2281 atggtagata gagcttgcgc agtttaacca ggatttccgg aagcgcggga gtttcgctga
     2341 caaacacaaa ctccttgaag gaccggtgat acatgtccaa agccctaatc aggcttgcgt
     2401 aaccccagac gttccgtttg gccgattcgc catagcttgt caaaatctcc gccgcggcat
     2461 cacgcaattc ggtttttcct gtaaactccg ccaaccttaa taagttctca caatgaaccg
     2521 ccacaccccc cgggatggcc tgatcagccc cggaaagagg acgggcaatg agccccggtt
     2581 catccgcagt taaataatac ttaccggatc catcgccaaa cttccgtaaa actgtctttg
     2641 tcaattcaag actctctgca agccaaagcg ggcgaaaatc agtttcgtac agatttaaaa
     2701 gtccccggac taaaaaagcg taatcatcaa ggaacgcagc ccccttagcc tgaccatcct
     2761 tataaacccg gaccagccgt ccggcggcgg gtctcatctt atccagaata aaaccggcgg
     2821 ccttttgggc ggcgcgataa tcggccgggt taccgaaaac ctggtaaact acagccaagg
     2881 ctccaatcat taatccattc catgaggtga tgatcttttc atcccggaac ggccggggtc
     2941 gggtttcccg aactgccaga agcgcggccc gcgcttgctg cagttttcgg ttgtattccc
     3001 attctttact ggggttcgcg gtttcattca gccggttcaa gatattgagt ccctcgaaat
     3061 ttccagcctc ggtcatccgg aaatggttga taaaaagttt ggctgtctcc gggtccaata
     3121 tcttttgaac ctcggcaatg ctccaaagga agaaccttcc ctcaccctcg gggctatccg
     3181 cgtcctgggt ggcgtaaaac cctccttccg gcgcggtcat ctcccggcgg atataggctg
     3241 cagtatcccg gacaacctcc ccgaactccg cttcttgtgt tatttggaac gcattggtgt
     3301 atatctgcag taactgagca ttgtcgtaaa gcatcttctc aaaatgaggc accagccaaa
     3361 tggcgtctgt ggcgtaacgg tgaaagcccc cgcccagctg atcgtagatt ccgcctcgcg
     3421 ccatccggcg taaggaaaac aaggccagat ccaccgcttc tttttgtccc gccgcagttc
     3481 ccacccgcaa aaaaagtccc aaaacgggag tgttgggaaa cttcggagcc ctgccaaatc
     3541 ctccattctg ccggtcaacc tctccgccca attcgccaat cgcccggagc ggcagttcgg
     3601 gaccgggtat cccggtttca ggagaggatc ccggttccgc ccgtaaatac tcgaccagct
     3661 cctgtccggc ctcggcaatc cgttcctgtt ccgagagcca tttttgatga accgcctcaa
     3721 tcagctcagg gaatgtcatt cggccgtaac ttgccgtggg tgggaaatag gtcccgccaa
     3781 agaacggccg gccctgatga tctataaaga ctgtcaaagg ccagccgcct tgaccggtta
     3841 tggccgccac agcctgttgg tatagctgat cgatatccgg ccgctcctcc cgatcgacct
     3901 tgatattgac aaaatattta ttcatcagcg cagcgatctc cggatcttca aaagactccc
     3961 gctccatgac atggcaccaa tggcaggccg aatagccgca ggaaagcaaa accggtttat
     4021 tttcggcttt ggctttttga aatgcatcat cgccccaagg gaaccaatca accggattgt
     4081 gggcatgttg aagaaggtaa ggggatgttt cgtaaattaa gcggttggta aatttggttt
     4141 cgggcattat ataaacatcc tttttgattt gtccttttta cattatcagt atgcccaaaa
     4201 tcggggattt tgcattgaag gggggcagtt cattctcttt ttatttcagg ataatccgat
     4261 cataaaagtc cctggcatat tgctccaggt gattcatttt ctccatttga ccggatattt
     4321 catagactgt atttcgaatc cgtaaagcct gggtgcttgc gttcatgtcg ccttccgcca
     4381 atcccttccc caagcgaaca ttattcgcca tttccaacga ccattttaca tggcgtgaga
     4441 aaccaccgtc aggtttcagg tattgttcca agtttttata agtgatcgtt aaaatatcta
     4501 aaatgtcatc ttcggcatag tcgcccaatt gtctttttaa aacacgcaac aggtcaatcg
     4561 gatttcgggt ccagcacata tcttcggaaa tatcattgtg cagggtatct agtaaacttt
     4621 gatagataag ctctgcacgt ggcaggggtt taccaatatc gcgatagaat aatgccagtt
     4681 tgaatgcgcc ggaaatcctg acatacggcc tgccttcacc ccataaaccg gtttgcgggt
     4741 cttgatgttc caacagataa ttgcaaatga attcgatgta tttttcggcc tccgcttccg
     4801 gtatagcgcg caggtaaaca ccgattgcct gaatattgtc accagccatc caggcataat
     4861 gccaggggcg ctcgtcaatc cattttttca aagcagctaa agattttaag caatccggta
     4921 atgtgtcacg gttttccccg ctgccaggta acgaatacaa cggatgacca ccaagcatgt
     4981 taagtgctgt acaactatac ataatcgccc tgccaaccat ccgttcaaaa ttacgcatat
     5041 cattctgaaa atcaaagaaa taaccttccg gggtttgacg tttttggaag aatgaaataa
     5101 cttggttttt catctcttcg ggcatctgct gtaatagatt tgagtttcct aaaatgtgaa
     5161 ccgcctgacc ggtagattca atatccggct ggaattccgg cgactttttg gaactgactg
     5221 cataataaaa cccgccgaaa ctcggatcat attgacctgc gagccaagtg tagaaatctt
     5281 caaacagcgg atcaaaagcg tttaagactt ccacgatttg tttcctctta gtcatcattt
     5341 gccacttctc ctctggttgg cgtcctaaac agtgtgactc gtacttcgcg cgaaaatctg
     5401 acagatattg tttactcccc ataattatac agaattaatc cattctttta tatggggttg
     5461 gattacttta gcaatcgcgc gtttaaatca tcatctaagg cttttcgcga ccatctccca
     5521 atctgcatag cggctgcatt cctcggcata ttttggtacc aaaggtattt tctaagtagt
     5581 gaatacccct cacccggata tgatataatt taactattcg cgtgatgcac ccgcattcat
     5641 aagcttaaaa aaggaattta tcaatgaaga acatcgtcat tcttgcaacc ggcggaacga
     5701 ttgccggagt ctccagctcg atcactgata ctgccaacta tcagccggcc atcctgccta
     5761 tcgaagccat tacgaaggat attgccgagc ccatacgaaa agtcgccaat atatccagcg
     5821 agcagattgc ccagattgac agcgttgaaa tgacgcatca aatctggttt acgcttgcga
     5881 atcgtattaa tgccttactg gcctcccccc aagttgatgg agcggttgtg actcatggaa
     5941 ccgacacgtt ggaggaaacc gcctattttc ttaatttggt tgtaaaaagc gacaaaccgg
     6001 ttgttgtggt cggggcgatg cgtccggcat ccgcgctgag ttccgatggc ccgatgaacc
     6061 tatataatgc cattattcta gcggctagtg atgaggcgac cggcaagggg gttttagtaa
     6121 cactgaataa ctccatcaat tccagccgcg aggttactaa gaccaacacc tccctccagg
     6181 atagctttaa agctccggaa cttgggtata tcggatacct aattgaaggg gaaccgcatt
     6241 tctacaggga acccacgcgt aaacacacct atatgagtga attcatcatt gatgacttga
     6301 cttgtcttcc tccggttgaa atcatttacg gacatgtcgg caacatgagt gcattagcgc
     6361 aagccgccgt ggccgctgga gccaaaggat tggtctatgc cggtatggga aatggaaata
     6421 tgtcaaaaat gatgaaaaat acttttatgg atattcagga acacggagtg gtcgtggttc
     6481 gaagcacccg ggtaagcagc ggtattgtaa ccagatacgg ggctgacaat gatgatctga
     6541 gcaacttcat cgtctccgac acccttaacc ctcaaaaatc aagcattctc ctgtccctag
     6601 cccttaccag gacgacagac cctgccgaaa tccagcgcat gttttggcaa tattagaatt
     6661 aacggattga aaatatgaac acataattcg cttataataa aaaagagcgg aatcttgaat
     6721 tgactcctct cttttctttg aaattccata cctaatttac ctttgagtta attggcttgt
     6781 tttatgaata ctgcctttga ctcgggatta agtttaacag tctcgccttc gatggtttcc
     6841 agcctgttat tcatcttgat tagcgacggt tttaccacat ccagtgggaa cctttatgaa
     6901 agccgataca cctgtgcccg agggctttgc aagttatgat ataccggctt gcaccattgc
     6961 cagggctgg
//
LOCUS       DASVMB010000328         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_263489,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000328 DASVMB010000000
VERSION     DASVMB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263489"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VF531_09335"
     CDS             <1..991
                     /locus_tag="VF531_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7714214.1"
                     /translation="VVKNSRTVGTVNIGFYGPFYFQDTDLAFINTLNRILLGVTVLAI
                     FLALFIGSFMAKRLSTPIAKVIQTTREIAGGNLDARSDSRTSIMEVNQLIEAVNNLAL
                     SLKQQESLRKRLTGDMAHELRTPLATLQSHLEAMLDGIWKPDSKRLKVCHDEIMRVNR
                     MVKDLERLAKYESDNSSLVKTRFDPAELIQQLLLNYEPEFRNKGVKLKFTADHLRIMA
                     DRDKLSQAIINLLSNALKFTPSGGEVAIRLEAGPETITIAVKDNGCGIAPEDLPYIFE
                     RFYRADKSRNRQTGGSGIGLTITKAIVEAHRGTIRVTSRVGEGTEFQILLPVG"
     gene            1504..2496
                     /locus_tag="VF531_09340"
     CDS             1504..2496
                     /locus_tag="VF531_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714215.1"
                     /translation="MKISEYQVSMNASHEYQEMYRVEEVQKHRMILPAAQQKTKPPTD
                     LLDFSKAAQRRLEQSKQNSGFGVQDDPLSRLSPRERVRFQTLDWLLGILSGGRLRLRM
                     VLPVERPKELVQVERKVSYQESEKLSFQASGVIKTADGKTIDFSAELNMSREFASQYC
                     TSVTETRELTDPLVVNFEGRAAELNETKVSFDLDSDGSLDQISFLKSGSGFLALDRNA
                     DGTINNGRELFGPGTGNGFGELTAFDQDHNGWIDENDAIYEKLRIWTKDPDGNDRLFA
                     LGEKGVGAIYLGNLNTQFSMKNAANQSQGELRRSGVFLREDGSAGTVQQIDLVI"
     gene            complement(2720..>4865)
                     /locus_tag="VF531_09345"
     CDS             complement(2720..>4865)
                     /locus_tag="VF531_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7714216.1"
                     /translation="QKGFGAPVPGFDHIPYGDLSVLETAFQAKPDYYAAFIVEPIQGE
                     GGIIIPPAGYLREALAICHRYGTLLIADEVQTGLGRTGKLFACEHDGITPDIITIAKA
                     LGGGILPIGACLATPEAYSEDFALKHSSTFAGNTLACRVGIKVLELLTRNDARLLKEV
                     ADNGAYLLKELTALQKAYPRVIAEVRGRGLMLGLELGATSEMLPGSLLGIMADQEILS
                     PIVSSYLLNVEKLRVAPTLNGASVIRLEPPLIISKEQCKTAVEAVTRMAQKLATGNTA
                     SFLGHLVGATIHTLAGSQPEIKEPVRPSGTPEEGRFAFLVHPLYLKNYWEFDSTLKYF
                     SEKQLGDLAWRWNDLVEPFPIAKTKIVSRTGQTAYGEFICVCRTSEELMNMPKEQVLK
                     ELGAAVRLAKARGAKIVGLGAYTSVVTKGGRELRDLGVALTTGNSYTVAAAAEATLEA
                     ARRLNIPISQTTAAVVGATGSIGRAVAILLAEQVHKLYLIGNPHNEEHSRRRLMKLVA
                     EIYQHLNEDNIITGGAILDFVRNHPRVPAPGSGSDAYLEFATAVAEESPLVYTVAIER
                     YLSQADVVVTATSQVTSFITPEMLKFGAVVCDVARPQDVSQEVKDARPDVLVIDGGVV
                     AVPGYPDLGWDFGFDKGLAYACMSETMMLALEHDYQHHSLGVDITLPTISQLQQLANK
                     HGFQLAGFRSFDQPLTTEAWENVIRARAAIPA"
BASE COUNT         1234 a         1198 c         1289 g         1144 t
ORIGIN      
        1 cgttgtcaag aattcacgga ccgtcggtac tgtcaatatc ggtttttatg ggccgtttta
       61 ttttcaagat accgatctgg ccttcattaa cactttgaat cggatcctgc tgggtgtgac
      121 cgtactcgct atcttcctgg ctttattcat cggtagtttc atggcgaaac gtttgagtac
      181 cccgatcgcc aaagtcattc agaccacccg ggagatcgcc ggcggcaacc tggatgcccg
      241 gagcgatagc cggaccagta tcatggaggt caatcaactg atcgaggcag tcaacaacct
      301 ggctttgagc ctcaaacaac aggaatcatt acgaaagcgg ctcaccggcg atatggccca
      361 cgagcttcgg acacccctgg ccaccttgca aagtcacctt gaggcaatgc tggacgggat
      421 ctggaaaccc gactcaaagc gtttgaaagt ctgtcatgat gagattatgc gtgtcaaccg
      481 gatggtgaaa gacttagaac gtttggccaa atacgagagt gataattcga gcctggtgaa
      541 gaccaggttt gatccggccg aactgattca acagctgtta ctcaactatg agcccgaatt
      601 ccggaataaa ggagtgaaac tcaagtttac agcagatcat ctaaggataa tggctgatcg
      661 ggataaactg agtcaagcca tcattaactt actgagtaac gccttgaaat tcaccccgtc
      721 cgggggagaa gtggcgatcc ggctcgaggc cggcccggaa acgatcacaa ttgcggtgaa
      781 agacaacggt tgtggaatcg cgcctgaaga tcttccctat atcttcgagc ggttttatcg
      841 ggccgacaag tcccgcaacc gccagaccgg aggttcggga atcgggttga ccatcaccaa
      901 ggcgattgtg gaggcgcatc ggggaacgat tcgtgtaaca agccgggtag gggagggaac
      961 ggaatttcag attctcttgc ctgtaggata gggtaggaac actgtttttc aatgaaaaat
     1021 gattgccctg aaaagcaaaa aagcctaaat gaaccgttcc atccggatag ttcaattagg
     1081 ctttttatag cttgtagacg tgattaacgg aaaaggcttg gctggtgccg gtaatccgta
     1141 ctccagcgtt tcatcaactt ccgttccagc tctgcctctc gatatttcag ttcaagctcc
     1201 agccagaaga acattgccag acaccccctt taaaaattgt atactgtatt ttgtatacaa
     1261 gatgattata ccaaggttag atacaattgt caatggatcc aaataagcga tatggaagga
     1321 tctgcggttt gctccccaaa attaagacct gggacctaaa aatgaaaata caactttagc
     1381 cgataaccaa attgggaacg gtttttgtat tccgtttcca acagggaggt cgaactttca
     1441 tgaaagccct tcggagcgag gcttttatga gatcacaact ctatggatgg aggggttagt
     1501 tcgatgaaaa tcagcgaata ccaggtcagt atgaatgcca gtcatgagta tcaggaaatg
     1561 tatcgcgtcg aagaagtcca gaaacatcgg atgatcttac ctgcggccca gcagaaaacg
     1621 aagccgccca ccgatctgct ggacttctca aaggccgccc aaaggaggtt ggagcaatca
     1681 aagcagaact cgggttttgg ggtgcaggat gatcccttat cccggctctc gcccagagag
     1741 cgggtacgat tccaaaccct tgattggttg ttgggcatac tcagtggggg caggctccgc
     1801 ctgcggatgg tgttaccggt agaacggccc aaggaattgg tccaggttga gcgaaaagta
     1861 tcttatcaag aatcggaaaa actttctttt caggcaagcg gggtcatcaa aactgcagat
     1921 ggaaagacca tcgatttcag cgccgagttg aatatgagcc gggaatttgc atcgcagtat
     1981 tgtacatcgg taacagagac ccgggaattg accgacccgt tggttgtgaa ttttgaaggc
     2041 cgggccgctg aattaaacga aacgaaagtt agttttgacc tggatagcga tggaagtttg
     2101 gatcagatct cttttctgaa atcgggcagc ggatttctgg ccctcgaccg gaatgcggac
     2161 ggaacgatca ataacggcag ggaactgttt ggcccgggta cggggaatgg attcggggag
     2221 ttgacagcgt ttgatcaaga tcataacggc tggattgacg agaatgatgc catttacgaa
     2281 aaattacgga tctggaccaa ggatccggac ggcaacgatc gtttattcgc attaggggag
     2341 aagggggtcg gcgccatcta ccttggtaat ctcaataccc aatttagcat gaagaatgct
     2401 gccaaccaat cccaaggcga gttacgtcgt agcggagtct ttctgagaga ggacggcagc
     2461 gccggaaccg tccaacagat tgatctggtg atataaataa ggtaaagatt gttcaatcgg
     2521 taaggcaacc attgggttca aacgttccct gaaaggggaa cgttttttaa ttccaattat
     2581 tgcaaaactc ctccagaata atatgcactg atgttggtaa tggaactatt tctgtaaaaa
     2641 aaagaggagg tcctgctttt cacgaaagct gtccccccat ctttttggaa gttaattttt
     2701 actttaagcc ccagtttgat taggccggga tggcggcacg ggcccgtatc acattttccc
     2761 aggcctctgt ggtcaacggc tggtcgaaac ttcgaaatcc ggccaattgg aaaccgtgtt
     2821 tattggccag ttgctggagt tggctgatag ttggcaacgt aatgtcaact cccagactat
     2881 gatgttggta atcatgctcc agcgccaaca tcatcgtttc cgacatgcag gcgtaggcaa
     2941 ggcctttatc aaaaccaaag tcccatccca gatccggata acccggaacc gcgaccacgc
     3001 ccccgtcgat caccagtaca tccgggcggg catccttcac ttcctggctg acatcctggg
     3061 gccgcgcaac atcgcatacc actgccccaa attttagcat ctccggggtg atgaagctgg
     3121 ttacttggct ggtggcggtg accaccacat ccgcctggct caagtaacgt tcgatggcca
     3181 ctgtataaac gagtggagat tcttccgcca cggcggtggc aaattccaaa taagcgtcgc
     3241 taccggaacc gggagccggt acccggggat gattgcggac aaaatctagg atggctccgc
     3301 cggttatgat gttgtcttcg ttaagatgtt ggtaaatctc cgctacgagc ttcatcaatc
     3361 tccgacggct atgttcttca ttatgaggat tgccgatcag atagagttta tgaacctgct
     3421 cggccagaag tatggctacc gcacggccga tggagccggt tgctcctact acagcggctg
     3481 ttgtttggga gatgggaata ttcagccgcc gggccgcttc caatgtggcc tccgcagccg
     3541 cagccacggt gtaactgttg ccggtggtta atgcaactcc aagatcacgg agttcccggc
     3601 cacccttggt taccaccgag gtgtaagcgc ctaaaccgac aattttggca ccccgcgctt
     3661 ttgccaggcg gaccgcagcg cctaactcct tcaaaacctg ttctttcggc atattcatca
     3721 gttcttccga ggtccggcag acacagatga actcaccgta agcggtctgg ccggtccggg
     3781 agacaatctt ggtcttggcg atggggaacg gctcaaccag atcattccaa cgccaggcca
     3841 aatcgcccag ttgcttttcg gagaaatatt ttaaggtgga atcaaattcc caataattct
     3901 taagatagag cgggtgcacc aaaaatgcaa accgcccttc ctccggggtg ccggaggggc
     3961 gaaccggctc cttgatctcc ggttggctac cggctagggt atgaatagtg gctcctacca
     4021 aatggcccag gaaggaagcg gtattacccg tggccagttt ttgtgccata cgggtaacag
     4081 cctccactgc ggttttgcat tgctcctttg agatgatgag cggcggctcc agccgaatga
     4141 ccgaggcacc gtttaaggtc ggcgctaccc gcagcttctc cacattcaac aggtaactgg
     4201 agacaatagg ggagagtatt tcctgatcgg ccataatccc caagagcgat ccgggaagca
     4261 tttcactggt ggcaccgagt tccagaccga gcatcaagcc tctgccgcgt acctcggcaa
     4321 taacccgggg ataggctttt tgaagggcgg tgagctcctt aaggagatag gcgccgttat
     4381 ccgctacttc ctttaataac cgcgcgtcat ttcgggtaag gagttccaat accttgatcc
     4441 cgacccgaca ggccagggtg ttaccggcaa aggtggatga atgctttaac gcgaagtcct
     4501 cgctataggc ttccggcgtg gccagacagg cgccaatcgg caggatgcct ccacccagag
     4561 ctttggcgat ggttatgata tcgggagtga tcccgtcatg ttcacacgca aataattttc
     4621 cggtacggcc taaacccgtt tggacctcat cggcaatgag cagggtccca tagcggtggc
     4681 atatggctaa ggcttcacgg agataccctg ccggtggaat gatgatcccg ccttcccctt
     4741 gaatgggctc tacaataaat gcggcataat aatccggttt ggcctggaat gcagtttcga
     4801 gcaccgataa atcaccgtat ggaatgtgat caaatcccgg aaccggggcg ccgaaacctt
     4861 tttgg
//
LOCUS       DASVMB010000329        10860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_556567,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000329 DASVMB010000000
VERSION     DASVMB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556567"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VF531_09350"
     CDS             complement(<1..726)
                     /locus_tag="VF531_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7714217.1"
                     /translation="MYDSFPDALPTLYETAPYIIIGSGIAGLYAALKLSSSGRVVLIT
                     KSSLTESNTHYAQGGIAAAISPNDSPDLHYQDTLMAGAGLCDPEAVRTLVDEGPVRVR
                     ELINLGVPFDRIQNGLALTREAAHSRNRILHAEGDATGRAISNTLIQLVRQKPIQIYE
                     QHYATALITQNGHCCGVVTIAEGRLHLFLGGAIILCTGGLGQIYSKTTNPQVATGDGI
                     ALAYRCGAAIRDMEFVQFHPTALY"
     gene            complement(759..1679)
                     /gene="nadA"
                     /locus_tag="VF531_09355"
     CDS             complement(759..1679)
                     /gene="nadA"
                     /locus_tag="VF531_09355"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7714218.1"
                     /translation="MIPEETIGMIATIQRLKKERGAVILAHNYQNDEVQDLADYVGDS
                     LALSQIAATTDAPVIIFCGVHFMAESASILAPDKTVILPAPTAGCPMAEMAEAEDVLK
                     WRKRYPQAAVVSYVNSTAAVKAVSDYCCTSANAITLVRRIPEKEIIFLPDQNLGRFVA
                     ERVPQKELHLWPGYCITHHRITPEEVTQAKAAHPDALVLVHPECRSEVWAQADFVGST
                     SQILQFAKTSSVRKFIIGTEMGILHNLKQDNPEKVFYLLSPGLVCPNMKQTTIPKILA
                     ALKDLAPVVTVAPEIRAAARKALDRMISLI"
     gene            1920..2315
                     /gene="folB"
                     /locus_tag="VF531_09360"
     CDS             1920..2315
                     /gene="folB"
                     /locus_tag="VF531_09360"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX7714219.1"
                     /translation="MNTTLRLKNMVFYGYHGVYSAEKELGQRIEVDVELVSDLTGAGK
                     TDDFDQTIDYVDIFTMVKEIVEEGQFNLMEGMATAILDQINSSYDLEQVTVRVRKPHP
                     PVGGLMDCLEVEVRSDPNRSHSTGEPELE"
     gene            complement(2447..3547)
                     /locus_tag="VF531_09365"
     CDS             complement(2447..3547)
                     /locus_tag="VF531_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7714220.1"
                     /translation="MKTYNHMKKPFSLLMTLAFVLLSVTGATIGLANPALAASGGDLL
                     GSSVVADIAEVSSPRVVWIETTYQNTQTYLTPFGLEQENTPAEGLGSGFFYDDNGYIL
                     TNAHVVANADSIEVTLKGQKTPLPATLVGMDQELDVAIIKVNLPNKITPLPLGDSDKT
                     RIGDWVVAIGNPYGLDHTVTLGIISAVGRPISVGGNNTIYDDMIQTDAAINPGNSGGP
                     LLDLKGEVIGINTAVNASGQGLGFAIPINTVKKILNELKTKGKLSHPYIGVSLVDVQS
                     DPQIRAYFGNNIYGALVRSVVKDSPAAKAGLRVGDLIMAMDDQDVASDVDLVTKIRKH
                     KIGDKINLLVMRRGSKITVPLTLGERPEQSGQ"
     gene            3758..4729
                     /locus_tag="VF531_09370"
     CDS             3758..4729
                     /locus_tag="VF531_09370"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7714221.1"
                     /translation="MEAILSRLKADFGQYVSGEELAKIAGMTRAGIWKYIEQLRSRGY
                     EIESAPHRGYRLTRLTPFLYPDEIHDGLKTKFFGSTIDFQFEVDSTNVRARALAVQGA
                     AEGTVVLAESQSGGKGRLGRNWNSPPGQGLWVSLVLRPKLPMAELAGINLLTAVAMSR
                     AIREVTGVQIDIKWPNDLVYHGRKLAGILAEVSGEMDRIHYLIVGIGVNVHQSVADFP
                     PELSDRAASLRMILDVVPSRKELLQAFLSHFEQAYLKLSESGLGDVISYARQHSATLG
                     RMVKINRGFGSAVTGEALDLAPDGSLLLRTDDGVITLHAGEIIETVK"
     gene            4749..5342
                     /locus_tag="VF531_09375"
     CDS             4749..5342
                     /locus_tag="VF531_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714222.1"
                     /translation="MEKRFLGKFTLVGLMVLLGLSLWLSGCGEPGYLDGLAGKRLGVY
                     LETEAGSVSDLNAALTDYISRRTKLEVVGTRFTGEVREDPATYSRLRNELGLDYLLLV
                     QLTDISPVRHRNDFDIAPGKMKMSLEYECKLNLTYKVIDLATEAPALIGQKRGQAEET
                     QSFHVDQGGFDFDFDPVDEEQLIEQAMFDAIHRSRLL"
     gene            5444..5938
                     /locus_tag="VF531_09380"
     CDS             5444..5938
                     /locus_tag="VF531_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714223.1"
                     /translation="MKKTGILTLTILLIMVLPVMAFGMTGPAVSENHGEISGVFISND
                     GGIQIGGKYGINNDLGLLLEVGQNDYSKLAIMYQLNPNVTIDGGMLCSQPFIGVMGAT
                     ALNKQLQGMGEIDLSYVNKEFVVNYQAGVEYAVKKDLDIRGGILGSTVGNSFAVGMGV
                     GYHF"
     gene            complement(6092..6859)
                     /locus_tag="VF531_09385"
     CDS             complement(6092..6859)
                     /locus_tag="VF531_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7714224.1"
                     /translation="MNPDKTARESTFISPAWLATIAAALLLLVAGWLIGTGGIARSGN
                     ETSRGIFVSGEGITKVRPDMARVILGVEASAATAAEAQTKNALIMAKILTSIKKLNIP
                     ARDIQTTEFNLFPERRFIQAEGRDQLIGYRVSNQIILSIRSLQLLGKVVDTAIQSGAT
                     NVNSVAFTVSDPARYERAALAAAVQNAQAKATALANRSKLKLKKVLYINESTSGVQPL
                     FMGANTLKRINMDAAAATPLEPGNVEIRASVQMGFAI"
     gene            7100..7645
                     /locus_tag="VF531_09390"
     CDS             7100..7645
                     /locus_tag="VF531_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7714225.1"
                     /translation="MIGYIFLAIILLLVVFSIGIYNNLVALRNRIDNAWHQIDVQLKR
                     RTDLIPNLVETVKGYAAHERETLDRVTEARSRLMRGGSVETQAQAQNELNGALKSLFA
                     VAESYPELKANQNFLMLQEELAGTENKIGYARQFYNDSVMSYNQAIQQFPGNLFAGFG
                     FPPREYFEIEPADRDLPKVRF"
     gene            7905..8792
                     /gene="htpX"
                     /locus_tag="VF531_09395"
     CDS             7905..8792
                     /gene="htpX"
                     /locus_tag="VF531_09395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX7714226.1"
                     /translation="MYEQISRNLRRSRILVACFLVIISALGYFFGAASGFGWLGFILA
                     LFIAVATSWFGYYYSDEVALSASHAKPLTKADNLYLYNLIEGLALAAGIPAPRAYLID
                     DTAPNAFATGRDPQHAAIAVTTGLMQKLNRAELEGVIAHEMSHIKNYDIRISTLVVVL
                     VGMAVLLSDWMMRSLWFGRGRGRNRDGGNFQGILALVGLIFMILAPLIAQLMQLAISR
                     KREFLADASGALLTRYPEGLASALEKLDADTEPLEVANKATAHLFIVNPLKEHGGFIN
                     HLFSTHPPIAERVKALRAM"
     gene            complement(9122..9793)
                     /locus_tag="VF531_09400"
     CDS             complement(9122..9793)
                     /locus_tag="VF531_09400"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HEX7714227.1"
                     /translation="MLRNDYIMRLIEQFTQAMALILGFKNAQKYQDALDLTAETIQQL
                     FGVDAKFIDTIPDEDLLVLLRTNDGIEPDKAIIIAALQKEAGDIFEAAGDSDSSYFRH
                     SKALNLLLEVYNHDTPTNLPDYLNETGIAGLIAKLDRYELPEATKLRLLLYYEKAGNF
                     AKAEDLLYDMIETGQPEIIDRGIAFYRRIALKSDQQLEAGNLPREEVQEGLAELERMK
                     EQNKA"
     gene            9976..>10860
                     /locus_tag="VF531_09405"
     CDS             9976..>10860
                     /locus_tag="VF531_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714228.1"
                     /translation="MRRIIGLSFLILLCGLFLNTLISVSSLAAVNQDQGAIDRLIEKT
                     KNDILSKKKREKSVLNNLMKQQQTLEKLGKNYAQVKNKFQTVEKNFNQTKQELAQLRT
                     NLNSLQETEKKRQDILNRRLVVIYKYGPENYLELLTQAENFSDLINRFNTLSYFVRND
                     LRLLDRVQETKSKVEATHQAVQAKSKQVEAEYRQVSTTRDQVSREQQKMSRQVSVTKN
                     ELDKIQSDRAKLEKALEEYEATSREIEAAIRKNEQTNPSTSLGTGRMSWPVRGRISDN
                     FGWRYHPILKRKKYHNGLD"
BASE COUNT         2668 a         2482 c         2858 g         2852 t
ORIGIN      
        1 atacagggcg gtgggatgaa attgcacgaa ttccatgtcg cggatcgccg caccgcatcg
       61 gtaggccaag gcaatcccat cgccggtcgc aacctgcggg ttggtggttt tggaataaat
      121 ctggcccaac cccccggtgc agaggattat tgccccaccc aggaagagat gaagtcgacc
      181 ttcggcaata gtaacgacac cgcaacaatg gccgttttgt gtaattaagg ctgtggcgta
      241 gtgttgttcg tatatttgaa tgggtttttg ccgtaccaat tggatcaggg tgttggatat
      301 agccctgccc gtggcatcgc cttcggcgtg cagaattcgg ttacggctat gggccgcttc
      361 tctggtcagg gccagcccgt tttggatccg gtcaaaggga actcccaggt tgatgagttc
      421 cctaacacgc acggggcctt catcaaccag tgtccgtacc gcttcaggat cgcataggcc
      481 ggcgccggcc atcagtgtat cctggtaatg gagatccgga gagtcgttgg ggcttatggc
      541 tgcggcgatg cccccctggg cgtaatgggt gttgctttcc gttaatgagc ttttcgtgat
      601 cagaacgacc cggccacttg aacttaattt taaagccgcg tataagccgg cgattccgct
      661 accaatgata atataaggtg cggtttcgta aagcgtgggt agtgcgtcgg gaaaggaatc
      721 gtacatgtga tagtgcctgc tttcagagtg gttttccgtc atattaagga gatcatccta
      781 tcaagggctt tacgagctgc ggcccggatc tccggcgcca cggttaccac aggggccaaa
      841 tctttcaagg ctgccaggat cttggggatt gtagtctgtt tcatattggg gcagactaaa
      901 ccggggctta ataggtaaaa aaccttttcg gggttgtcct gtttcagatt atgtaaaatt
      961 cccatctcgg ttccaatgat gaatttacgg actgatgatg ttttggcgaa ttgcaggatc
     1021 tgggaggtgc tgcctacaaa atccgcctga gcccacactt cggaacgaca ttccggatgg
     1081 accaggacaa gagcgtcggg atgggctgct ttagcttggg ttacttcttc cggtgtaatt
     1141 cggtgatggg tgatacagta ccccggccaa agatgcagct ccttttgggg gactcgttcc
     1201 gcaacgaaac ggccgagatt ttggtcgggc aggaagatga tctctttttc tggaatcctc
     1261 cgtaccaaag taatggcatt ggcggaagtg cagcaatagt cactcactgc cttgaccgca
     1321 gccgttgaat taacatagga aaccactgcc gcctgcgggt aacgttttcg ccacttaagc
     1381 acatcctcgg cttcagccat ttccgccata gggcaacctg cggtcggagc cggcaggatc
     1441 acagtcttgt caggtgccaa aatagaagcg ctttctgcca taaagtgcac accgcaaaag
     1501 atgataaccg gggcatcggt agtggcggcg atttgactca atgcgagtga atcgccgacg
     1561 taatcggcta agtcttgtac ctcatcattt tggtagttat gcgcgagaat aacagccccc
     1621 cgttctttct tgagccgttg aattgttgcg atcattccga tggtttcctc aggtatcatg
     1681 gggtataaaa acctccatat caaatccggc ggtaagcgat ttaacctaat tttacttggg
     1741 gaagcttttt ttgtcaatct cccggcatga cgcggcacta tatcatgctt actgtttact
     1801 ttaaatcatg ttctataaaa tgtaaggaaa tattcatcat cttcacttca caccgaccca
     1861 aaagtggatt aaaatgggat tgatatttaa agctcagttc tccggctgga gggatggcta
     1921 tgaacaccac actacgtttg aagaacatgg ttttctacgg ttatcatgga gtttattctg
     1981 ctgaaaaaga gttgggacag aggattgagg ttgatgttga actggtctcg gatctcactg
     2041 gcgcgggcaa gacggatgac ttcgatcaaa ccattgatta tgtcgacatt tttaccatgg
     2101 tcaaggagat tgtggaagaa ggccagttta atctgatgga gggtatggcc accgcgattc
     2161 ttgaccagat aaactccagt tacgatctgg aacaagttac cgttagggta aggaaacctc
     2221 atcctccggt tggcgggtta atggactgcc ttgaagtgga agtgagaagc gatccgaatc
     2281 gctcccattc aaccggggag ccggaattag agtaatcaag gcttgtttcc tcgtttttaa
     2341 aggaattttg gctgggggca agggctactc cgggaacaat agaaaacggt gacgcaccaa
     2401 cgcgtcaccg tttagatttt agaaaatcat tacgggaggc aaaagcttac tgaccggatt
     2461 gttccggccg ttcgcccagg gtcagcggga ccgtgatctt ggacccgcgg cgcatgacaa
     2521 gcaggttgat cttatcgccg attttgtgtt tgcgaatctt ggtgaccaga tcaacatcac
     2581 tggcgacatc ctgatcatcc atggccatga tcaggtcgcc cacccgcagc cccgccttgg
     2641 cggcagggga atcttttacc accgaccgga ctaaggctcc gtatatattg tttccgaagt
     2701 aggcccgaat ctggggatcg gactggacat cgaccaggct gactccgatg tagggatggc
     2761 taagtttacc cttggtcttc aactcattta aaattttctt gacggtattg ataggaatgg
     2821 caaatcccaa cccctgaccg gaagcgttga cagcggtatt gattccgatt acctccccct
     2881 tgagatcgag caggggaccg ccactgttcc ccgggttgat ggcggcgtcg gtttgaatca
     2941 tatcatcgta gatcgtatta tttccgccaa ccgagattgg ccgccctacg gcactaataa
     3001 tccctagagt gacggtatga tcaagaccgt aaggattgcc gatggctaca acccagtcgc
     3061 cgatccgggt tttgtcggaa tcacccaatg gcaacggcgt gattttattc ggcagattga
     3121 ctttgatgat ggcaacgtcc agttcttgat ccattccgac cagtgttgcc gggagcggag
     3181 tcttttgccc cttgagggtg acctcgatgg agtcggcatt ggcaaccacg tgagcattgg
     3241 tcaggatata gccgttgtcg tcgtaaaaga aaccgcttcc caaaccttcg gcaggagtat
     3301 tctcttgttc caggccgaag ggggtcagat aggtttgagt attctggtag gttgtttcaa
     3361 tccagacaac tctgggacta ctgacctcgg caatgtcggc caccaccgac gaaccgagaa
     3421 gatcgccgcc ggaggccgcg agtgccggat tggccaggcc aatcgtagca ccggttacac
     3481 ttagcaggac gaaggcaaga gtcataagga gcgagaaggg ttttttcatg tggttatacg
     3541 ttttcatgtc ggtttcacct caaatttctt cccagtaata ttattttggt tatttgataa
     3601 gttctaacga attcctccga tcatagttcc tggttttttt gggggaggat gcagtaaatg
     3661 gtaggaatgg cgagaacaca gaaaaacttg ggaaccgggt tgcgtatgag ctgatttttg
     3721 agtatacttc agttggaacc atcggaggag gatttttatg gaggcaattc tttcacggct
     3781 caaagccgat ttcgggcagt atgtatccgg agaggaattg gcaaagattg ccggaatgac
     3841 ccgggcgggg atctggaaat acattgagca actacgttcc cggggttacg agatcgaatc
     3901 cgcgccccat cgcggttatc ggttaacccg gctgaccccg tttctatatc ccgatgagat
     3961 ccatgacggt ttaaagacca agttcttcgg gtcgaccatt gactttcaat tcgaagtgga
     4021 ttcgaccaat gttcgagccc gggctttggc ggtgcaaggt gcagcggaag gcacggtggt
     4081 gcttgccgag tctcagtccg ggggtaaagg gcggttggga cgcaactgga attctcctcc
     4141 gggtcagggg ttatgggtta gtttggtttt acgtcccaag ctacctatgg cggaattagc
     4201 cggcatcaat ctattgacgg cagtggccat gtcccgggca attcgtgaag taaccggagt
     4261 gcagatcgat atcaagtggc cgaatgattt ggtttatcat ggccgtaagc tggcgggcat
     4321 tctggccgag gttagcgggg agatggaccg gatccattac ctcattgtcg ggatcggcgt
     4381 caatgtacat cagtctgttg cggatttccc ccctgagctt tctgaccgtg cagcttcgtt
     4441 gcgaatgatt ttagacgttg tgccatcacg gaaagaattg ctccaggcgt tcttaagcca
     4501 ttttgaacag gcatatctta agttatcaga gtccggactg ggcgatgtta tttcctatgc
     4561 gcggcaacat tcggctacat taggccgtat ggtcaagatc aaccgggggt ttggcagtgc
     4621 agttaccggt gaagcgttgg atctggcccc ggacggtagt ctccttcttc ggaccgatga
     4681 cggtgtcatc acacttcatg ccggtgagat tatagagacg gtgaaatgac agggaggggg
     4741 tcaaacgaat ggaaaagagg tttttgggaa aatttacctt ggtagggctg atggtccttt
     4801 taggattaag tctttggctc agcggttgcg gcgaaccggg ctacctggac ggtttggccg
     4861 gaaaaagatt aggggtttac ctggagaccg aagccggctc agtttctgac ctaaacgccg
     4921 cgcttaccga ttatatcagt cgccgcacca agcttgaagt ggtgggaacc cgcttcacag
     4981 gggaagtccg ggaggatccg gcaacctata gccgcctccg gaatgaactg ggattggatt
     5041 acttactttt ggtccaattg acggatatca gtccggtccg tcacaggaac gacttcgata
     5101 tcgctcccgg gaaaatgaag atgagtctgg agtacgaatg caagctgaac ctgacataca
     5161 aggttattga tttggcgact gaagcacctg ccttaatagg ccagaaacga ggccaggccg
     5221 aagagaccca atcgtttcat gttgatcagg gcgggtttga ttttgatttc gatccggtcg
     5281 atgaggaaca attgatcgag caggccatgt tcgatgccat tcatcgttcc cgtttgcttt
     5341 aacaaaaaat tcgaattcaa taaaaaggga atcgtgaaaa agaggcgaaa ctaactgcgg
     5401 ttagttaatc aaataaatca atcaaatcaa gggggtattc ataatgaaaa aaacaggaat
     5461 tcttactctt acaattttac tcatcatggt tcttcccgtg atggcatttg gcatgaccgg
     5521 gcctgcagtt tccgaaaacc atggtgagat cagcggggta ttcatcagta atgatggcgg
     5581 gattcaaatc ggcggtaaat atggtatcaa taacgatctg ggtttacttt tagaggtcgg
     5641 tcaaaacgat tattccaaac ttgcaatcat gtaccagctc aatcccaatg tgacaatcga
     5701 cggaggtatg ctctgttccc agcctttcat cggtgtcatg ggtgccactg cacttaataa
     5761 gcaattgcaa ggtatgggtg aaatcgatct ttcctatgtc aataaagaat ttgtcgtgaa
     5821 ttatcaagcc ggcgtagaat acgccgtcaa aaaggatctc gatattcgtg gcgggatttt
     5881 aggctcaacc gtggggaata gttttgctgt tggaatgggt gtgggttacc acttttaatc
     5941 gggatatgtg aattgaagaa ctccggaaca aagcatgagt gatcgagtta accaaatgaa
     6001 ccaaaccgaa acgggctgcc caaaacagag ttgggcagcc cgttttttct gcgccggcat
     6061 gggcgcaatt catcggattg gtccaccgat tttagattgc aaagcccatt tgtacactgg
     6121 cgcggatctc tacatttccg ggttccagag gagtggctgc cgccgcatcc atatttattc
     6181 gcttgagggt gtttgccccc ataaataatg gttgaacacc ggaagtgctc tcgttgatgt
     6241 ataggacttt ttttagtttt agtttcgacc ggtttgccaa ggcggtggct ttcgcttgag
     6301 cgttttggac cgccgcggct agagcagccc gctcatagcg agccggatcg gacactgtaa
     6361 aagctacact gttgacattg gtggctccgg attgaatcgc agtatccact accttgccta
     6421 atagctgcaa agagcggatt gacaggatga tttggttgga gacacggtag ccaatcaact
     6481 gatcgcgtcc ttccgcttga atgaaacggc gttccgggaa caggttgaat tcggtggttt
     6541 gaatatcccg cgccggaata ttaagctttt taattgatgt caggatttta gccataatta
     6601 acgcgttttt tgtctgggct tcagctgcag tagcagcgga tgcttcgact cccagtatga
     6661 cccgggccat gtccggacgg actttcgtaa taccttcacc actgacaaag ataccccgac
     6721 ttgtctcatt gccacttctt gcaataccac cggtaccaat caaccatcct gccaccaaaa
     6781 gcagcagagc agcggcgatc gtcgccaacc aggccggaga aataaacgtg gattctctcg
     6841 cggttttatc aggattcata gatcttcctc ctttcggctt ccagagtatc atattttgac
     6901 aaaggaggtt tccttcctgc cgtaccgaaa gaaataaagc caaaaatatt ttgccatggg
     6961 aaattttatc aagtggttaa aggaatcatg ggttcgttag ggaatataat atattctgag
     7021 tccgtatcgg tccggataaa aatcagggtt gatctttacc aaacaatccg gaacccaaga
     7081 tttgaggtgg ttatggttgt tgatcggata catttttttg gcaatcatct tattgctggt
     7141 agttttcagc attggaatat acaataattt ggtggcctta cgaaaccgga tcgacaatgc
     7201 ctggcaccag atagacgtcc agctgaaacg aagaacggat ctcatcccca atctggtgga
     7261 gacggttaag gggtacgccg cgcatgagcg ggagacgctg gatcgggtga ccgaagcccg
     7321 ctcccggttg atgcggggag gatctgtaga gacgcaggct caggcccaaa atgagttgaa
     7381 cggagcttta aagagcttgt ttgctgtggc tgagagttat ccggagttaa aggcgaacca
     7441 aaacttttta atgcttcagg aagaattggc aggaactgaa aataagatag gttacgccag
     7501 gcagttctat aacgattcgg tcatgagcta caatcaggct attcagcaat ttcccggcaa
     7561 cctgtttgcc ggattcggtt tcccgccccg ggagtatttc gagatcgaac cggcggatcg
     7621 ggatctcccc aaggtccggt tttaagatat ttggagagaa tgacatttca acttggttga
     7681 tattggtttc ctcgcatccg gcatctgatg cctgcgtgca ggaactcacc ccgcttagct
     7741 tttcttctcc tgggatcatt tggaggagat ggtaagatac gctttcccgg cggtttctcc
     7801 tctctttacc ttgcgtaaag ggagggggga ctggggtgaa tttgcaggtg aagatgtcga
     7861 aggctttatg tgaaatataa atgacagggt aagtgtaagt gttgatgtac gaacagatat
     7921 cccgaaattt acggcgttcg cggatcctgg tcgcttgttt tctcgtcatc atcagtgcac
     7981 tgggttattt ctttggagcg gccagtggtt tcggttggct gggctttatc ctcgccttat
     8041 tcattgctgt ggccaccagc tggtttggtt attactacag tgacgaggta gctctatcag
     8101 cgagtcatgc caaacctctg accaaggcgg ataatctcta cctctataat ctcatcgaag
     8161 gattggcatt ggcagccgga ataccggctc cccgcgctta cttgattgat gataccgcac
     8221 ccaatgcctt tgcaaccggg cgcgacccgc aacacgcggc gatcgccgtg accaccggtt
     8281 taatgcaaaa actaaaccgg gctgagctcg aaggagtaat cgcccatgaa atgagccata
     8341 ttaagaatta cgatatccgc atctcaacct tggttgtagt gctggtagga atggcagtgc
     8401 tgctctcaga ctggatgatg cgaagtttgt ggtttggcag ggggcgcggt aggaaccggg
     8461 atgggggtaa cttccaagga atcttggctt tggttgggct gatatttatg attttggcgc
     8521 cgcttatcgc acagttgatg caactggcga tatcacgcaa aagggagttt ttagccgacg
     8581 cctcgggagc attgttgacc cgatatccgg aaggtttagc cagtgctttg gagaaattgg
     8641 atgcggatac cgaaccgctg gaggtagcca ataaggctac ggctcatttg tttatcgtca
     8701 atcccctcaa agaacacggc ggttttatca atcatctatt ttcaactcat cccccgattg
     8761 ccgagcgagt aaaagcactg cgcgcgatgt aaaagaaaag tttgatccca atatatttat
     8821 aattttaaaa tgtttggtaa tattaggatg ccaagtagat caattaggca ctagggaaca
     8881 ggcaattggc aacaggaaag agatgtgggt ctttttatat gtcatgcttt gtatgccctg
     8941 taaaacacca tgctccgctg tattttttaa agacgataac gatagctaat ctcctccaag
     9001 ggatagaggt tgcttttagg cctaagcctt gcccttctca cactttacgc ctgttgcccc
     9061 acgttttatg aaacgcacta cccacgtccg ttgccttttg ccttttttca tgcctctcac
     9121 ctcatgcctt gttttgttct ttcattcttt ccaattccgc cagtccttcc tggacttcct
     9181 cccgcggcag gttccccgct tccagttgtt gatcgctctt caatgctatt cgccggtaaa
     9241 aggcgattcc ccggtcgatg atctccggtt gacccgtctc gatcatgtca tagagtagat
     9301 cttccgcctt ggcgaaattc ccggcctttt cataatatag caacagccgt agtttggtcg
     9361 cctccggcaa ctcgtaacgg tccaatttgg cgataaggcc agcgatgccg gtttcattta
     9421 aataatccgg caggttggtt ggagtatcgt ggttataaac ctccagtaat aagttgagcg
     9481 ccttggaatg gcgaaagtaa ctggaatcac tatccccggc ggcctcaaaa atatccccgg
     9541 cttctttttg aagtgctgcg atgatgatgg ccttatccgg ctcaattccg tcattggtcc
     9601 ggagtagaac caacaggtct tcatcgggga tggtatcgat gaatttggcg tctaccccga
     9661 atagttgttg aattgtttcg gcggtgagat ctaaggcgtc ctgatacttt tgggcgtttt
     9721 taaaacctaa gattagagcc attgcctggg tgaactgttc aatcagccgc atgatatagt
     9781 cgttacgtag catggttcct ccggattata ttgttcatat gacctgcgtt aacttatagg
     9841 ttcgacagtt cctttcgggt tccttttttg gcaggatcca tcctgtattg cttccttatc
     9901 catcaaatgc caacggttat ttagaggatg agccttgctg tttgtcgaac cccatcaaaa
     9961 aaaggggggg cggccatgcg gcgtatcatc ggtctttcgt tcttgatttt gctctgcggg
    10021 ttgtttctta atacactgat ctcggtctcg tcccttgccg ccgttaacca agatcaagga
    10081 gcaatcgatc ggctgattga aaagaccaag aatgacattt taagcaaaaa gaaacgggaa
    10141 aaatcggtac tgaataacct catgaagcaa cagcaaaccc tggagaagtt gggcaaaaat
    10201 tatgctcagg ttaagaataa gttccagacc gttgaaaaaa atttcaatca aaccaaacaa
    10261 gaattggcac aacttcggac caatctgaat tcacttcaag agacggaaaa aaagcgtcaa
    10321 gacattctta accgccggct ggtggtgatc tataaatacg gacccgaaaa ctacctggag
    10381 cttttaacgc aggcagaaaa cttctccgat ctgatcaacc gcttcaatac cctgagttat
    10441 tttgtcagga acgacctgcg attattggat cgggtccaag agacgaagtc caaagttgaa
    10501 gccacccatc aggcagtgca agcaaagagc aaacaggtgg aagcggagta tcgccaagta
    10561 tccacaaccc gggatcaggt ctcccgtgaa caacagaaaa tgtcgcgcca ggtttcagtc
    10621 accaagaatg aattggacaa gatacagagc gaccgggcaa aactggaaaa ggctcttgaa
    10681 gaatatgagg ccacttcccg tgagattgaa gcggccatcc gcaagaacga gcagacgaat
    10741 ccttctacct cactgggaac gggccgcatg agctggccgg tacgggggag gatctccgat
    10801 aatttcggtt ggcgttatca tccgatcttg aaacggaaaa aatatcataa tgggttggac
//
LOCUS       DASVMB010000330         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_630122,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000330 DASVMB010000000
VERSION     DASVMB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630122"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF531_09410"
     CDS             complement(<1..284)
                     /locus_tag="VF531_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714229.1"
                     /translation="MVTLSRQCRTALLFFLPGTILVAFFSLTKPFLTARPAKLPGRFA
                     GSNLVDYTTLQNSWHSSWIEILKSQGHSDTIPKENSNFRLYERRYGKVRL"
     gene            complement(314..1585)
                     /locus_tag="VF531_09415"
     CDS             complement(314..1585)
                     /locus_tag="VF531_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7714230.1"
                     /translation="MKKARQRMIVVSTLIFITVGAFGWWWQGNVRQGRYKDLPAALYI
                     MTLVKFNYYQQVSLPGLFQSYMDTGNISGMLKSLQDPYTEFLDENDFAELRKVTTGSF
                     GGIGIYLPEKGDLIITGVVKGSPGEKAGLKVGDRIIAINRVPVGKPAVAIAKIRGEAG
                     TRVLLRIVRGDGANRREVEVSIVRSQITIPTVELKFTSDPVMGRYAYLRIDQFAETTA
                     SDLESRLREIDNTPDCAGIMLDLRANPGGLLDSAVDVTSNFLPKGTPVLHIYHRGRLF
                     ETKRVVQPIPHKKLPMVTLVDYWTASASEIVAGALKDQKRSVLVGTHTFGKDLIQEVK
                     GLPGGVGVKITVFNYLTSGKVNIHKKGVQPDRVVEIPGAVDRLLKTGDSRLFYEMEAE
                     QQQAALELLREKAQARARATVYANLPVRKAG"
     gene            complement(1627..>2712)
                     /locus_tag="VF531_09420"
     CDS             complement(1627..>2712)
                     /locus_tag="VF531_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7714231.1"
                     /translation="VSWGVTADDLQKQIDQTRKKLSQTKVRENSVLNRLVRTQQELNN
                     ISSNLERLNSNLGNTERRIATIKAQLTTAENELNQIKDQIGGRKGVLDERLVAIYKYG
                     YQSFMEVLFTAKNFPEFITRFEMVSSFVKGDIQIIKALQSQRALITQKRVEIAKKQKE
                     LENQKVLYSKLQDQTKRQQTRQLSVIRDQKEELAQVQQDRKLLEESIDRMEEQSKQLE
                     AQIRDLSNKNGTSLGTGKMIWPVPDARITSYFGWRVHPILKKRKYHSGLDLAVVYGTP
                     IKAADNGVIIFSGRNGDYGNMIAIDHGSNISTVYGHCSVLIAKVGQKVTKGDVIAKVG
                     STGLSTGPHLHFEVRKNGVPVNPLSYL"
BASE COUNT          626 a          731 c          632 g          723 t
ORIGIN      
        1 ctcaacctca ccttgccata gcgccgttca tataaacgga aattgctgtt ttccttagga
       61 attgtatcgg aatggccttg ggattttaaa atttcaatcc atgaagaatg ccaggagttt
      121 tggagcgttg tatagtcgac caagttggat ccggcaaatc ttcccggaag tttggccggc
      181 cgggccgtaa ggaacggttt tgtgagagaa aaaaaggcca ccagtatggt accaggtaga
      241 aaaaaaagca atgccgtccg gcattgcctc gaaagcgtca ccactggaaa ccactccttc
      301 ggcgtacctt aggtcacccg gcttttcgca cggggaggtt tgcgtatacg gttgcgcgag
      361 cacgagcttg agccttctca cggagcaatt ctagcgcagc ttgttgctgt tcagcctcca
      421 tctcgtaaaa aagccggcta tcaccggttt ttaaaagccg gtctacggcg ccgggaatct
      481 ccaccacccg gtccggttgc acgcctttct tatgaatatt caccttccct gaagttaaat
      541 agttaaaaac tgtaattttt acccctactc caccaggaag tcccttgact tcctggatca
      601 gatctttgcc aaaggtatgc gtcccgacca gaaccgaacg tttttgatcc ttcaaggcac
      661 cggcaacgat ctcggaagca ctggcagtcc agtaatccac taatgtcacc atcggcaatt
      721 ttttatgcgg tatcggttgg accacccgtt tggtctcaaa cagccgacct cgatgataaa
      781 tatggagaac cggcgtgccc ttgggtaaaa aattgctggt cacatcgacc gcggaatcga
      841 gcaaccctcc cggattggcc cgcagatcca gcatgattcc cgcacaatcc ggggtgttat
      901 cgatctccct caaccgggat tccagatcgg acgcggtagt ctcagcaaat tgatcgatcc
      961 ggagataggc gtatctcccc attaccgggt cggatgtaaa cttcaattcc accgtgggaa
     1021 tggtgatctg actccggaca atgctgacct cgacttcgcg acggttagcc ccatctcccc
     1081 gaacaatccg gagtaaaacc ctggtccctg cctccccccg gatcttcgca atggccacgg
     1141 cgggtttccc aaccggaacc cggttgatcg caatgatcct gtcccccacc ttaaggcccg
     1201 ccttctctcc cggtgaacct ttaacgactc cggtaatgat cagatcgccc ttttcgggca
     1261 gatagattcc aatccctccg aaagacccgg tggttacttt acggagttcg gcgaagtcgt
     1321 tttcatccaa aaattcggtg taaggatcct gcaacgattt caacatcccg gaaatattac
     1381 ccgtatccat ataggactgg aatagccccg gaaggctgac ctgctggtaa tagttgaact
     1441 taaccagggt cattatgtac aaagcggcag gtaagtcctt ataacgtccc tgtcggacat
     1501 tcccctgcca ccaccatccg aaagccccca cggtaataaa gatcaacgtg gaaacgacga
     1561 tcatacgctg ccgggctttt ttcaacagac caaccgcccc ttctgtttct attatagata
     1621 gctaagctat agatagctta acggattgac agggacacca ttctttctaa cctcaaagtg
     1681 gagatgcgga ccggtgctca aaccggtact tcctactttc gcgatgacat cccccttggt
     1741 gaccttctgt cccactttgg cgattaatac ggaacagtgg ccataaaccg tggaaatgtt
     1801 cgagccgtga tcgatggcga tcatattccc gtaatctccg ttacggccgc tgaagatgat
     1861 gactccgtta tccgcagctt taatcggggt tccgtaaacc actgcgagat ccagtccact
     1921 gtgatattta cgctttttga ggatgggatg cacccgccag ccgaaatacg aggtgatccg
     1981 ggcatccgga accggccaga tcattttgcc cgttcccagg gaagtgccgt ttttattgga
     2041 taaatcgcgg atctgagctt ccaactgctt ggattgctct tccatccggt cgatggattc
     2101 ttcaaggagt ttgcgatcct gctggacctg ggccagctct tctttctggt cccggatcac
     2161 cgagagctgt cgggtctgtt ggcgtttggt ttgatcctgc aactttgagt agagtacctt
     2221 ttgattctcc agttcttttt gtttcttggc gatctctacc cttttttggg taattagagc
     2281 ccgttgtgac tggagcgcct tgatgatctg gatgtcgccc tttacaaaac tactgaccat
     2341 ctcgaagcgg gtgatgaatt cgggaaaatt tttcgcagtg aacagtactt ccataaaact
     2401 ttgatatcca tatttatata tagcaacaag acgctcatcc agaacacctt tcctaccgcc
     2461 aatctggtcc ttgatctggt taagttcgtt ttcagccgta gtgagttgcg ctttaatcgt
     2521 cgcgatcctg cgctcggtat tgcctaaatt agagttgagc cgttccagat tggaactgat
     2581 attatttagt tcctgctgtg tccggactag ccggttgaga actgagttct ccctgacttt
     2641 agtctggctt aacttttttc tggtctgatc aatctgcttc tgcagatcat cggcggttac
     2701 gccccaactt ac
//
LOCUS       DASVMB010000331         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_334969,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000331 DASVMB010000000
VERSION     DASVMB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334969"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1202
                     /locus_tag="VF531_09425"
     CDS             180..1202
                     /locus_tag="VF531_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714232.1"
                     /translation="MIRSHFRSFKFLLTIALLFVVWGLAGCGSGSGYRLIYSTMLDEN
                     QQIHLSASNGSGRIRLTTGNWAYDPVWSPDGRSIVFVNETSDGDELFSIAANGSNLRQ
                     LTDGLKNGENVSPDWSPDGRKIAFVSGRDGNPEIYVMNADGSGVVRLIANDSVDNEPH
                     WSPDGKRIVFVSKRDGNSEIYVMNFDGSGARNVSKNKALDDSPAWSPDGSRIIFRSGR
                     NGGSSIFTVQPDGAHLTRLSRYHYRDTSPAWSPDGRRILFVSEREGNDEICIMNANGS
                     HQKALTVNLAPDRSPAWSLDGKTIAFASFRDNNWEIYLMSADGKNQRNITNSPVNEFD
                     PVWSPK"
     gene            complement(1388..2305)
                     /locus_tag="VF531_09430"
     CDS             complement(1388..2305)
                     /locus_tag="VF531_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7714233.1"
                     /translation="MNQDKNGLKILILGGTGFLSGTLAKKALLKNNEVWTLSRGNHVA
                     EDDGRNVLIADRHEADRFKRVIEAANIRWDTVIDCIGMNPEDARQDIDVLSHFTKQII
                     FISTDSVYDPFHKKIPQNEDSDYYLTEGYGGNKRLCELEFLHSDMGETFWTIIRPGHI
                     FGPGSQLGCFPLHARDKELLHKLREGKTISLVGGGHFLQQPIFAPDLCDMLLSFIGNE
                     KTYNQIFCVAGPEIIESKKYYEMIAEILGVAINFAEIPIDAYYSAHPEDAPFLCHRVY
                     DLSKLRSTGAAIPSTSMIDGLRLHVKSLL"
     gene            complement(3172..>3617)
                     /locus_tag="VF531_09435"
     CDS             complement(3172..>3617)
                     /locus_tag="VF531_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7714234.1"
                     /translation="CSCTPVQIQRYQSRISGPLLDRFDLQIEVPRMQDFEMDTVSTGP
                     TSAEIRSRVEAARRIQSDRYQGTPYFCNAYMSSKDLRNYCHLDPSGRELLHQAVQRFS
                     LSARGYTRILKLARTIADLEGSTDIEMQHLAEAIQYRCLDRKLML"
BASE COUNT          970 a          822 c          779 g         1046 t
ORIGIN      
        1 tgatcttagc tatcaagcca ccgcgggttt tgcccgtttt atcctggaag cgctgaatcc
       61 gaaagcttcc ctggaaaaaa cgaatatcga aaaaatggaa caacttattg gcatgaaact
      121 ccgttatatc atcgccgctt actgatagat gaattttgtt tcgaattcga ggcgccgcaa
      181 tgatccgtag tcatttccga tctttcaagt ttttgctcac catcgcgtta ttatttgtgg
      241 tttggggatt ggcagggtgt ggttccggct ccggttaccg gttgatctac agtaccatgc
      301 tggacgagaa tcaacagatc cacttgtccg cctcgaatgg cagcggacgg atccggctta
      361 ctaccgggaa ttgggcttac gatccggttt ggtcgcccga tggccgttcg attgtctttg
      421 ttaatgaaac ctccgatgga gatgagcttt ttagcatagc ggcgaacggg agcaatttac
      481 gacagttgac tgacggattg aaaaacggtg aaaacgtaag cccggattgg tcgccggacg
      541 ggcgtaagat agcctttgtt tccggaaggg acggcaatcc ggagatctat gtgatgaacg
      601 ccgatggctc tggtgtggtc cggctaatcg cgaatgattc ggtcgataac gaaccccatt
      661 ggtcgccgga tggaaagcga atcgtctttg tctccaaacg cgacggcaac tccgagatct
      721 atgtgatgaa cttcgacggc agcggagcac ggaatgtcag taaaaataag gccctggatg
      781 attccccggc atggtcaccg gatggctctc ggattatttt tcgttccgga cgtaatggtg
      841 ggtcttccat tttcaccgtc caaccggatg gggcgcatct cacccgtttg agcaggtatc
      901 attatcggga tacttcccct gcttggtccc ctgatggccg gaggatcctc tttgtctccg
      961 agcgtgaagg aaatgatgag atctgtatca tgaacgccaa cggctctcat caaaaggcat
     1021 taacagttaa cctggctccg gatcgctccc ctgcctggtc cctcgatggt aagacgatcg
     1081 ctttcgcttc atttcgggat aataactggg agatttatct gatgagcgcc gacggcaaga
     1141 atcagcggaa tatcaccaac agcccggtga atgagtttga tccggtgtgg tcgccgaagt
     1201 gagagtttaa gtccagagtc aaaagttgaa aaaacgtatt tggttcattc cattgtgtgg
     1261 cttttataaa aatatagcta aaaagttttt taattttttt tatcggccat ttccggtaat
     1321 aatttatatt tttttctgtt cttttatgag ctcgatcatt ttcttatgaa ctgattatat
     1381 tagttattta caatagactt ttcacatgca acctcaatcc atcaatcatc gaggttgaag
     1441 gaatcgctgc tccggtactc cttagttttg ataaatcata tactctatgg cataagaatg
     1501 gcgcatcctc aggatgagcg gaataatagg cgtcgatcgg tatctctgca aaatttattg
     1561 ctacacccaa tatctctgca atcatctcat agtatttttt tgattcgatg atttccggac
     1621 ctgcgacaca gaatatctgg ttgtaggttt tttcatttcc aatgaagctc agcaacatat
     1681 cacaaagatc cggggcaaat atcggttgtt gaagaaaatg acctcctcct accaaggaaa
     1741 tggtttttcc ttcgcgcagc ttatgaagga gttccttatc gcgcgcatgt aaaggaaaac
     1801 atcccaattg ggagccaggt ccgaatatgt gaccgggacg gatgattgtc caaaaagttt
     1861 cacccatgtc actatgtaag aattccagct cacaaaggcg cttattgccg ccgtatcctt
     1921 cagtcaagta ataatcgctg tcttcatttt gggggatttt tttatgaaat gggtcataca
     1981 cggaatcggt tgaaataaaa ataatctgtt tggtaaagtg acttaacacg tctatatcct
     2041 gcctcgcgtc ctccggattc atcccaatac agtcaataac cgtatcccat cttatattgg
     2101 cggcctcgat gaccctttta aaacggtctg cttcatggcg atccgcaatc aaaacattcc
     2161 gtccatcgtc ttcggcaaca tgatttcccc tagataaagt ccaaacctca ttatttttca
     2221 gtaatgcttt ttttgccaag gttccgctta ggaagcccgt acctcctaag ataaggatct
     2281 ttagtccgtt tttatcttga ttcatcagct aatcttcctt tgatatttta tattacaatt
     2341 atacaataaa cttttcatcg gcggctacta atcgagcgcg gaacatccat tcgcagcatc
     2401 aacttccgac ctaaacaccg gtattgaatg gcttccgccg gatagtgcat ctctcccctc
     2461 cctgacaacg ctgacgaatc cattgccggc atacttgaga tgctttgagt cccccagaag
     2521 atactttagg acaaaaaata accaaggtac aaatgtacca tggtatgcga aaacaaacag
     2581 gtatatggcc gaacatgctt gccgaccgtg aaaaggctct ttctattgat ttagaaatgg
     2641 acaactacgg ttagattatt catccttgag aagtgtaggt tcaatcatcg aatacctata
     2701 agtacgatta gttaattccg attatcaata gaattaagat taaaaatagt agaaatgcta
     2761 attcctcatt aaatacgcca ataccaccca ttcttttagc cccctttaca ttttcatccc
     2821 aaaaacagaa caagatgatt attattgtaa aattccaaat gaagctccta tcaaaaagca
     2881 ttggtggttt cgaatagtaa aacctcataa taacggtgtt tgtttgttat agaatatgat
     2941 tacaacagtg tggatgaaat atggtaaagg tactttttta tagtaaagtt gcatattgtt
     3001 acttatttag taaaataagc atgcaacttt ggcagtaata attgaaaaag ccaggctttc
     3061 atacctgtcg aattttttca aaaaattact atactttatt taatgagtaa actcatatca
     3121 aaacatgccc cttttttaac attcgcaaac attcaattga caattaccgc tttacaacat
     3181 caacttccga tccagacacc ggtattgaat ggcttccgcc agatgctgca tctcaatatc
     3241 cgtgctcccc tccaaatcgg caatggtccg ggccagtttc aatatccggg tgtagccgcg
     3301 ggcgcttaag gaaaatcgct gcaccgcctg atgaagcagt tccctgcctg atggatctag
     3361 atggcagtag ttacgaagat ccttgctgct catataggca ttgcagaaat acggggttcc
     3421 ttggtaacgg tcgctttgta tcctgcgggc cgcctctacc cggctcctaa tctccgccga
     3481 agtcggtccg gttgaaaccg tgtccatctc gaaatcctgc atcctgggaa cttcaatctg
     3541 gaggtcgaac cgatccagca acggcccgga gatacggctt tggtaccgtt ggatctgtac
     3601 cggggtgcaa ctgcagc
//
LOCUS       DASVMB010000332         6033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_41296,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000332 DASVMB010000000
VERSION     DASVMB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41296"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="serS"
                     /locus_tag="VF531_09440"
     CDS             <1..746
                     /gene="serS"
                     /locus_tag="VF531_09440"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003392668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7714235.1"
                     /translation="LVNFMLNLHTGEHGYTEIFPPYLVNRASMTGTGQLPKFEEDMFK
                     CTNGEYYLIPTAEVPVTNLHREEILDAAQLPIKYAAYTACFRAEAGSAGRDTRGIIRQ
                     HQFNKVELVKFTRPEDSYAEHEKLVHDAEEVLQLLSLPYRVMLLCTGDQSFSAAKCYD
                     LEVWLPSANTYREISSCSNFEDFQARRANIKYRPAPGAKPEFVHTLNGSGLAVGRTMA
                     AILENYQNADGSVTVPEALRPYLGVDKLS"
     gene            complement(857..1996)
                     /locus_tag="VF531_09445"
     CDS             complement(857..1996)
                     /locus_tag="VF531_09445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7714236.1"
                     /translation="MKRYSSLGIFFFSLFLATTVFATGGSLTPPSNLPMGSKGQTTTD
                     STGQNGIQTPNGQNLKTGRVIAVGDLNFQSGLGAKILADPEYPWANVRKIFETGSIRV
                     GNLEVPLSNRGSKYTEKTWILRADPRTVQALVKAKFQVVTLANNHIMDFGPVALGDTI
                     ATLDTAGIAHAGAGMNLEEARRPALVTAPDGTRFAFLSYSLVFPDLFWAKKNRPGTPY
                     GLPELFIADIKQAKTFADHVVVSFHWSEELRFYPKPYQKAYAHQCIDAGADIVLGHHP
                     HVLQGLEVYKGGLIAYSLGNFAFGTFSRRCTDSIILAVDYDAAGLNQAVLYPVNVNNH
                     EVNFQTRLRHGDDAERALQDLRSYSAEFKTTIRSEGDTGVIQIRE"
     gene            2171..2422
                     /locus_tag="VF531_09450"
     CDS             2171..2422
                     /locus_tag="VF531_09450"
                     /inference="COORDINATES: protein motif:HMM:NF045434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714237.1"
                     /translation="MVSNSLTVFGIMLDDRGQNAPDVQEVITRHGSEIIGRMGVPSPS
                     KKKGFITLLFEGELTQVEAFRKEIEEIDGATVQTMSFPQ"
     gene            2439..3368
                     /locus_tag="VF531_09455"
     CDS             2439..3368
                     /locus_tag="VF531_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX7714238.1"
                     /translation="MKQGLGEFAMRCFYVWLKAVRAPFFTAAIIPVLVGASLAYRMGR
                     FNLLGLIIALVIVIAAHAGANLLNDYYDALGSDPLNESPTPFSGGSRLIQTGILKRSD
                     YLHGAALAYGISLVTVALAAWLYRNPLILGLGVAGAGLGISYSSAKISGMSRGWGEVA
                     VGVGFGPMAVAGSFLLQANTLTIRAFAAGIPVAFLIMGVLILNEFPDYPADDRAGKRT
                     WIVRTGVESLGVWVYLGVISLAYLTLIIGVVSGLFPVRVLYSCLTIPLAVWIGLKLWR
                     FRRDAARLIPAMAGNIGLHLLTGILICLGFWFK"
     gene            complement(3361..3936)
                     /locus_tag="VF531_09460"
     CDS             complement(3361..3936)
                     /locus_tag="VF531_09460"
                     /inference="COORDINATES: protein motif:HMM:NF039407.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HEX7714239.1"
                     /translation="MDIEIIYPALKKPLSIIEIVRKICFWIFLIAAYCCPIINICVGG
                     KAWSIIVLWSLWFVWNTFLTRPLVENNLISQTTHLLLNSCILLVLIEVFLSSGWAEFV
                     VPIICFGMLVILGIIFFLDISKQKQNMMPMLWVIGGSLVAIVSAFLGWSSMNWPTIVL
                     GSTAFALLTASIVVLRLQFILELKKRFHTFT"
     gene            complement(3926..5419)
                     /locus_tag="VF531_09465"
     CDS             complement(3926..5419)
                     /locus_tag="VF531_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714240.1"
                     /translation="MKKKESKYQLYIKALSDNEKRLLASYFKRLEQYILLPQKEKQKM
                     LSDFERAILYYSGTLLPLEEALTRLDPSNLGGFYARPPILWYPLDDAAKIYPLSMNRN
                     QMAIFRLSAYMNEDVIPEILQMALTFTIKRFPYFATTIKKGVFWHYIDSTKKRYSVEP
                     ETEIPCKPMNVSISGSQSFRIIYYKSRISVEFFHILTDGTGGTIFLKTLIGEYLRLCG
                     IQIPYVDGVLDINSTPTAAESSNDFSKALPSERSTGFADKPAIQMSGRLAGVRPCQIL
                     HFEFNSMKLQAIAKQKGVSVTAFILALMFLAGKAATDETEGSIHIQVPVNMRKFYESN
                     TIRNFSLYCSIRMPLCDINDLDGILPEITKQLSENASKKSMNDMMNATVKLVRILKFI
                     PLFIKRPAARLVYGFLGDKVFSNTLSNLGVIKIPEEMRSYVKKIDFVLGTGITNRASC
                     SMVTLGDTATLSITKMTADPSFENKLYILFKESGLLPDVKGSELYGY"
     gene            5736..>6033
                     /locus_tag="VF531_09470"
     CDS             5736..>6033
                     /locus_tag="VF531_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7714241.1"
                     /translation="MEYQTLYRRFRPRNFSDFVGQNHVITTLSNALATDRVAHAYLFT
                     GPRGTGKTSAAKIFAKALNCQSPVHSEGKPGEPCNTCSNCVRINEGTFMDVFEID"
BASE COUNT         1543 a         1369 c         1509 g         1612 t
ORIGIN      
        1 ctttggtcaa ctttatgctc aatctgcata ccggggagca cgggtatacg gagattttcc
       61 cgccttatct ggtgaatcgg gcttcaatga ccggcaccgg ccagctcccc aagttcgaag
      121 aggatatgtt caaatgcacc aacggcgagt attacttgat ccccaccgcc gaggttcccg
      181 taaccaatct gcaccgtgag gagatcctgg acgccgccca attgccgatt aagtatgcag
      241 cctataccgc ctgtttcagg gctgaggccg gttccgccgg gcgtgacacc cggggcatca
      301 ttcggcagca ccagtttaac aaggtggagt tggtcaagtt cacccgtccg gaggattctt
      361 atgcggagca tgagaaattg gtgcacgatg cagaggaagt tctgcaattg ttaagtttgc
      421 cttatcgggt gatgttgctc tgcaccgggg atcagagctt ttcggcggcc aaatgttatg
      481 atcttgaggt ctggttgcca agcgccaaca cttaccggga gatctcaagt tgcagcaatt
      541 ttgaggattt ccaggcccgc agggcgaata tcaaatatcg cccggctcca ggggctaagc
      601 cggagttcgt tcataccctg aatggttcgg gactggcggt ggggcgtacc atggcggcaa
      661 tcctggagaa ttatcaaaat gccgacggta gtgtgacggt tcctgaggcg cttcggccgt
      721 atctgggagt agataagcta agctaggctt agataaaaag cttaaactgg attaaccaaa
      781 aaacgggccg ccggagttac cggacggtcc atttttgtat gcccggcacg agtgtcgttt
      841 tatcccaaag gaaggatcac tcccggattt gaatcactcc ggtatcgccc tcactacgaa
      901 tggtagtctt gaactctgcc gaataactcc ggagatcctg gagtgcccgt tcggcatcgt
      961 cgccgtggcg aaggcgggtt tgaaagttca cttcgtggtt gttcacattc accgggtaga
     1021 gtacggcctg gtttaggccc gcagcgtcat agtccacggc caggataatg ctgtcggtgc
     1081 atctcctcga gaaggtgccg aaggcgaaat tacccaggct ataagcgatt aatccgcctt
     1141 tatacacttc cagcccctgg aggacatggg ggtgatgccc aagaacaata tcggcgccgg
     1201 catcgataca ctgatgggcg tatgccttct ggtaaggttt agggtagaaa cgaagctcct
     1261 cgctccagtg aaaggatacc accacgtggt cggcgaaggt cttggcttgc ttgatatcgg
     1321 cgatgaacag ctcggggagg ccatagggag ttccgggacg attctttttt gcccaaaaaa
     1381 gatccggaaa aaccagtgaa taagagagga aagcaaaccg ggtgccatcc ggcgccgtca
     1441 caagagccgg ccgccgggcc tcttccaggt tcatccccgc tccggcgtgg gcaattccag
     1501 ctgtatcaag cgtggcaatg gtatcgccca gagccacggg gccaaagtcc ataatatgat
     1561 tgttggccaa agtgacaacc tgaaatttgg ctttcaccag cgcctggacg gtccgcggat
     1621 ccgcccgcag gatccaggtc ttctccgtgt atttactgcc gcgattggag agcggaactt
     1681 ccagattgcc tacccggata gagccggttt caaaaatctt tcgtacgttg gcccaagggt
     1741 actcgggatc tgccagaatt ttcgcaccca atccggactg gaagtttaga tcaccgaccg
     1801 caatcacccg gccggtttta agattttggc cgttgggggt ctgaatccca ttctgtccgg
     1861 tggaatccgt agttgtttga cccttcgaac ccatgggaag gttcgatggg ggggttaacg
     1921 aacctcctgt cgcgaaaact gttgtggcga gaaataagga aaagaaaaag atgcctaacg
     1981 agctgtatcg tttcatcggg atcgtccttt caagtttccg tggatgtgat tatattgcag
     2041 ccgtgccgag ggccggcttt cagattttat tccctgatga attcgcgata ccattgattt
     2101 ttcctgcctt aatccggtgg agggactatt ttggggctga atatgggcat aataaaggag
     2161 aggtgatcat atggtgagta attcattgac agtattcgga atcatgctgg atgaccgggg
     2221 acagaacgcc cccgatgttc aggaggtaat tacccgtcac ggcagcgaaa tcattggccg
     2281 gatgggtgtt cccagtccct ccaagaagaa aggattcatc acactgcttt ttgaggggga
     2341 gctcacacaa gttgaggcct tccgcaagga aatagaggag attgatgggg ccaccgttca
     2401 aaccatgagt tttccccaat aaacaggtga ccgggaagat gaaacaagga ttgggtgagt
     2461 ttgccatgag atgcttctat gtatggttga aggcggtccg ggcgcctttt tttacagctg
     2521 cgattattcc ggtgctggtg ggcgcttctt tggcataccg aatggggagg tttaaccttt
     2581 tggggctaat catcgccctg gtcatcgtca tcgccgccca tgccggagcc aatctattaa
     2641 acgattatta cgatgccctc gggagtgatc cgctgaatga atcacctaca ccgtttagcg
     2701 gtggcagtcg gttaattcag accggaatcc ttaaacgatc cgactatctc catggcgcgg
     2761 cgctggctta cggcatcagc ttagtgactg tagccctggc agcctggctt taccgaaacc
     2821 ctttgattct gggattagga gtcgccgggg ccggtttggg aatatcctat agctccgcca
     2881 agatttccgg aatgagccgg ggatgggggg aagtggcggt gggcgttggg tttggcccca
     2941 tggcggtagc cgggagtttt ctgcttcaag ccaatacttt gactatcagg gcgttcgcgg
     3001 ccgggattcc ggtggctttt ctgatcatgg gagtcctgat tctaaatgaa tttccggatt
     3061 acccggcgga tgatcgggcc ggtaaacgga cctggatcgt ccggaccgga gttgagtcat
     3121 tgggagtatg ggtctatctg ggagtcataa gtttggccta tttgacttta attattggtg
     3181 tcgtaagcgg actctttccg gtccgggtac tctattcatg cctgactatc ccgctcgcgg
     3241 tctggattgg tctgaaatta tggcgttttc gtcgcgatgc cgcccgattg attcctgcca
     3301 tggccggaaa tattgggctg catttattga cgggaatatt aatctgcctg gggttttggt
     3361 tcaagtaaat gtgtgaaatc ttttctttag ttccaagata aattggaggc gaagaaccac
     3421 aatacttgca gtaagcagtg caaaggctgt tgaaccaagt acaattgtag gccaattcat
     3481 cgatgaccat cctaaaaatg cgctgacgat agcaaccaac gagccgccga ttacccacag
     3541 catgggcatc atattctgtt tttgctttga tatgtccaaa aagaaaatga tccctaaaat
     3601 cacaagcatg ccaaagcata taatcggaac gacaaattcc gcccagcccg agctcaaaaa
     3661 cacttcaatt aaaacaagga gaatacagct attcaataac aaatgtgttg tctggctgat
     3721 taagttattt tcaactaacg ggcgcgtcag aaatgtattc catacaaacc acaacgacca
     3781 cagcacgata atactccagg cttttcctcc tacacagatg ttgataatgg gacaacaata
     3841 tgcggcaatt aaaaatatcc aaaaacatat tttcctaact atttctatta tagacagcgg
     3901 ttttttcagc gccggataaa taatctcaat atccatataa ttcacttcct tttacatcag
     3961 gcaacaaccc actttcctta aacagaatat agagcttatt ctcaaacgac ggatcggcag
     4021 tcatctttgt gattgaaaga gttgccgtat ctcccagcgt aaccatggaa caggatgccc
     4081 tgtttgtgat gcccgttccg agaacaaaat ctattttttt gacgtaagat ctcatttctt
     4141 ccggtatttt tatgacgccg agattcgata aggtattact aaaaactttg tcgccgagaa
     4201 atccataaac gagcctggct gcaggacgct ttataaatag cggtataaat ttcagtattc
     4261 gaaccaactt aacggtagca ttcatcatat cgttcattga cttcttagat gcattttcac
     4321 ttagctgttt tgttatttcc ggcaaaattc cgtccaaatc attgatatcg cacaagggca
     4381 ttctgatgct gcaatagagt gaaaaatttc tgattgtgtt cgactcataa aacttacgca
     4441 tgttgaccgg aacttggata tgaatacttc cttcggtttc gtctgttgcg gctttccctg
     4501 cgaggaacat taaagcaaga atgaaagccg ttacagaaac acctttttgt ttagctatag
     4561 cttgaagttt catactgtta aattcaaaat gaagaatttg gcatggtctt acgccggcaa
     4621 gtcttccact catctgaata gctggcttgt ctgcaaatcc ggttgacctt tcagaaggca
     4681 gagcctttga aaaatcattt gaggattcgg cagcggtcgg ggtgctattg atatcaagga
     4741 ctccatcgac gtatggaatt tgaattccac aaaggcgcag atattcgccg atcaaggttt
     4801 tcaaaaaaat tgtaccgccg gtgccgtctg tgagaatatg aaaaaattca acgctgatgc
     4861 gacttttata ataaataatc cgaaaggatt gagaaccgct gattgataca ttcatcggtt
     4921 tacatgggat ctccgtttca ggttcaaccg aataccgctt ctttgtcgaa tcgatataat
     4981 gccagaagac cccttttttt attgtagttg caaaataagg gaagcgcttt attgtaaagg
     5041 ttagagccat ttgcagtatt tcaggaatta catcctcatt catatatgca gagagcctga
     5101 aaatagccat ttggttccta ttcatcgaaa ggggataaat ttttgcggca tcatcaagag
     5161 ggtaccacag aataggcggc cttgcataga atcctcctaa atttgaagga tcgagtctcg
     5221 taagcgcttc ttcaagagga agaagggttc cggagtagta caagatggct ctttcgaaat
     5281 ccgagagcat cttctgcttt tctttttggg gaagcaaaat atattgctcc aaccgcttga
     5341 agtaacttgc aagcagtctt ttttcattat cggaaagtgc cttaatatat agctgatatt
     5401 ttgattcttt ttttttcatt agtatatacc tttgagacta aaggctataa taaaataata
     5461 tttatagaaa gtataccata caagattaaa aagttatatg taaactcatt agtgttacat
     5521 aaaaacatgt tctatctgta cctctgcgtg gcttggtcgg ttattaggaa caattgtttc
     5581 gcgtgcgtcc ttatgcatgg agatcaatcg ttcaaaaact gactgttcgg aggaaacgac
     5641 ggttgataca gttgtcccgt atccgggtag aatcgagcaa ggagttttgg ggtggatagc
     5701 gaataagaag gggaatgttt ctttgggagg aaccaatgga ataccaaact ttataccgcc
     5761 gcttccggcc ccgtaatttt tcggattttg tcggtcaaaa tcacgtaatc accactttat
     5821 ccaatgccct tgccaccgat cgggtggccc acgcctatct tttcaccgga ccccgtggga
     5881 cgggcaagac gagcgcggcg aagatctttg ccaaagcgct caactgtcaa tctcccgttc
     5941 atagcgaagg aaaacccggc gaaccttgta atacctgttc caactgcgtt cggatcaatg
     6001 aagggacttt tatggacgtt tttgaaatcg atg
//
LOCUS       DASVMB010000333         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_630702,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000333 DASVMB010000000
VERSION     DASVMB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630702"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF531_09475"
     CDS             <1..542
                     /locus_tag="VF531_09475"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7714242.1"
                     /translation="VCTCGAIPMGALLQTQKQLKTRGLTLNAGFAIQMPGSYLVKYGA
                     FSVEKQKNIFNKEKEKIETIVRIIKNQQEHKIERNNFIINGIGNLVYPSMLLKFPTLD
                     RNFTVNEKCTHCNTCEKICPVQNIKIIDGRPNWQGNCEHCLACIQWCPAEAIQYGTQT
                     IDRKRYHHPDVSVRELYRI"
     gene            981..1379
                     /locus_tag="VF531_09480"
     CDS             981..1379
                     /locus_tag="VF531_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7714243.1"
                     /translation="MKGFKLWISLKRSLLGAVFVNLPQFHPYAKMLPQAGCCIIVCGD
                     KEKQGMIGFLIEDCSAAIQNMLLAAHGAGLGAVWCGLYPVPKLTKAISRLLELPRTVM
                     PVGMVAVGRKNEQREAPDRFDYTKVHFEKW"
     gene            1403..2059
                     /locus_tag="VF531_09485"
     CDS             1403..2059
                     /locus_tag="VF531_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7714244.1"
                     /translation="MGFSGVIFDFNGTLFWDTELHNEAWNIFIAENRLKKLSDAEKNL
                     KMHGKTNQEILPMLFGKELTPEEVRVFIQRKETIYQELCLKLKMDLAPGVVPLLDFLK
                     LRNIAFTIATSSGIGNLRFYFEHLNLEKWFDFDKVVYNDGTMKSKPDPEIFLTAMAKI
                     QKAPREVIVFEDSLAGIAAAERAKPGKIIIVASTNEDYGRWDYPVIKSFAAVDREMFL
                     "
     gene            2210..3016
                     /locus_tag="VF531_09490"
     CDS             2210..3016
                     /locus_tag="VF531_09490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7714245.1"
                     /translation="MTIKALFFDMDGTLLTSEAKIASSTQNALKICREAGIQVFLATG
                     RPPSLRIMLHLTDPQRELFASGGVFYNGGCIIVNGIKEYNPLPNRALESAVDTVLNFP
                     GVNMAIQMVDEKQSYRYELSENEYRGMGVEQDSILHFKYPWEYEAVKIVVFSDHDLDE
                     QIYRQLQNKIGAMANLYFVQNNRVIDIVDKNTSKKLAIDRVAGICGLNHDEIAVFGDD
                     LNDIEMLSGNRYSIAMGNACQAAKEVAAYITDSNDDNGIYHALKEYLKLI"
     gene            3062..>3382
                     /locus_tag="VF531_09495"
     CDS             3062..>3382
                     /locus_tag="VF531_09495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714246.1"
                     /translation="MELTEKRICLEHAQPDDARELLRIQTTAFAVDLEKYGSGPSGFD
                     SLEWQIDMIESGNYYKIILDGEKIIGGMVVFDKGEDHLHLTRIFIDPDYQNMGLGQLA
                     VCMVE"
BASE COUNT         1024 a          662 c          808 g          888 t
ORIGIN      
        1 cagtatgtac ttgtggcgcc attccaatgg gggcgttgtt acaaacccag aaacaattga
       61 aaacaagagg gttgacctta aacgccggtt ttgcgattca gatgcccggg agctatttgg
      121 taaaatacgg cgcgttctct gttgaaaaac aaaagaatat attcaataaa gaaaaagaga
      181 agatcgaaac tattgtaaga atcattaaga accagcagga acacaaaata gagcggaata
      241 attttatcat caacggcatc ggtaatttgg tttatccgtc aatgttgctc aaatttccaa
      301 cccttgatcg aaattttacc gttaatgaga agtgcaccca ttgcaataca tgtgaaaaga
      361 tatgtccggt tcaaaatata aaaataattg acggaagacc caattggcaa ggaaactgtg
      421 agcattgcct ggcctgtatc caatggtgtc ctgcagaagc cattcaatac ggcacgcaga
      481 cgattgacag aaaacgatat catcatcccg atgtatcggt tagagagtta taccggattt
      541 aaaataaagc tctttggagg aaataacgga ttcgataaga tgatctggca agattccact
      601 gcacttgatt cggaaaaata aaaaaggact aaaagccctt ttgctttcag ggggccctcg
      661 gaacggatcg gttcatcaaa aggaatggca ggaaaatgct gggttgtaca gaatattcaa
      721 agtaggagat catcaagatt tccatcgtta aaaatcccca atctaccaat cgagcgataa
      781 tagtctatct caaaaagatt agcgaggtgg aaaaatgaac gaaattggta aaaccaagct
      841 gcgaatatgg accgggttcg ctttggtcgt cgggatcatt gcaatcgtga ttatttccgg
      901 atatctcttc ttctctcaga tgctcccgga ttaaaagaca cttgtccgaa cacccatcaa
      961 tttatggaat tgattcattc atgaaaggat ttaagctatg gatatctttg aagcgatctt
     1021 tactcggcgc agtattcgta aatttaccgc agtttcaccc ttacgccaag atgttacctc
     1081 aggcggggtg ctgtatcatt gtttgtggcg ataaggaaaa acaaggcatg ataggctttc
     1141 ttatcgagga ctgttctgcg gccatccaaa acatgctact ggcggcgcat ggggccggtt
     1201 taggcgctgt atggtgcggt ttgtatccgg tgccgaaact gactaaagct atatcccgac
     1261 tgctcgaatt accaagaacg gtaatgcctg tgggcatggt ggcagtgggg aggaagaacg
     1321 agcaaaggga agcccccgac agatttgact ataccaaagt acactttgaa aaatggtgac
     1381 tcaaccggag ggatattttt ttatgggttt tagcggcgtg atttttgact ttaacggaac
     1441 tttattttgg gataccgaac tgcataacga ggcctggaat atattcatcg ctgaaaaccg
     1501 gctcaaaaaa ctgtcggatg cggaaaagaa tctaaaaatg cacggtaaaa ccaaccagga
     1561 gatccttccg atgttattcg gcaaagaatt aacacctgag gaggttcggg tttttatcca
     1621 aaggaaagaa accatatatc aggagctttg cttaaaatta aagatggatt tggcgcccgg
     1681 ggtagtacct ttgctcgatt ttctgaaatt acgcaacatc gcatttacca tcgccacatc
     1741 ctcgggcatc ggtaacctgc ggttttattt tgaacatcta aatttggaga aatggttcga
     1801 tttcgataag gttgtatata atgacggcac gatgaagagt aaacccgatc cggaaatctt
     1861 cttgacggcg atggcaaaaa tccaaaaagc ccctcgggaa gtcatcgttt ttgaggattc
     1921 cttggcaggg attgcggcag cggaaagagc caaacccggt aagattatta tcgtcgcttc
     1981 cacgaatgaa gactatggcc gctgggacta tccggtcatc aagagctttg ccgcagtcga
     2041 tcgggaaatg ttcttgtaat gccatcgtca tattcaatat cacgtaggtc actgtaacag
     2101 cattttaagg gaacagaata gagaagcagt agaatggctt gatatgttca tggaataaag
     2161 tgaatgatga ttacagattc atcaacatga tttgaaagga acgaatactt tgactatcaa
     2221 agcattattt ttcgatatgg atggaacgtt gctgacctcg gaggcaaaga tcgcatccag
     2281 tactcaaaac gctttgaaga tttgtaggga agcaggaatt caagtattcc tcgccaccgg
     2341 ccggccgccc tcattgagaa ttatgttaca tctaaccgat ccgcagcggg agcttttcgc
     2401 ttcaggtggt gttttctata acggcggctg tatcattgtg aacggtatca aagaatataa
     2461 tccccttccg aatcgggctc ttgaatcagc tgtcgatacg gttcttaatt ttcccggggt
     2521 taatatggcg attcagatgg ttgacgaaaa acagagttat cgctacgagc tttccgaaaa
     2581 tgaatatcgc ggaatgggtg ttgagcagga ttcgattctt catttcaagt atccgtggga
     2641 gtatgaggcc gttaaaattg tggtattctc cgatcacgat ctggacgaac aaatctaccg
     2701 gcagcttcaa aacaaaatcg gcgctatggc caatctttac tttgtccaaa acaaccgggt
     2761 gattgatatc gtagacaaga acacctcaaa aaagctggcc attgacagag ttgcagggat
     2821 ttgtggacta aaccacgatg aaatcgctgt ttttggtgat gacttgaacg atattgaaat
     2881 gctcagtggt aatcgatatt ccatagccat gggtaatgct tgccaggcag caaaagaagt
     2941 cgcagcctac atcacggaca gtaatgatga taacggcatt taccatgcac tgaaggaata
     3001 tcttaagctg atttagagga cgcttggcta cagggaatag ctatgtcaaa ggagtattgg
     3061 agtggaatta acagaaaaaa gaatttgtct ggaacacgcc caacctgatg atgcccgtga
     3121 attattgcgg attcaaacca ccgcctttgc cgtcgatctg gagaagtatg gcagtggccc
     3181 gtcgggtttc gactctttag aatggcaaat cgatatgatt gaatcgggaa actattataa
     3241 aatcatcctg gatggtgaga agatcattgg cgggatggtc gttttcgata aaggagaaga
     3301 ccacctgcat ctcacccgga tctttatcga tccggattat cagaatatgg gactcggtca
     3361 attggcagtc tgtatggttg aa
//
LOCUS       DASVMB010000334         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_483092,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000334 DASVMB010000000
VERSION     DASVMB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483092"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VF531_09500"
     CDS             complement(<1..856)
                     /locus_tag="VF531_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714247.1"
                     /translation="MELELYQIKARKKPVYGKQILTYLILTLIAIITLVPLAWALSSS
                     LKTHSEIYAFPPQWIPKVLNWSNYVRAWTMVPFGRFLLNSLYMSSTVTIMQLIVSSLA
                     AYAFARLRFPGRDTLFLLYLGAMMIPGQVLIIPNFILMRFFGWIDSYKALILPEVFNM
                     FTIFCTFLLRQYFLTLPGELEDAAKIDGCSRFQTFIRIIMPLSKPALATVAIFAFKDQ
                     WNNFLWPLIVINTPEKMPIQVGLTFFRGEFNTEWEVLLAGTIIALIPVIILFLFGQKY
                     FVKGFSISG"
     gene            complement(874..1800)
                     /locus_tag="VF531_09505"
     CDS             complement(874..1800)
                     /locus_tag="VF531_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714248.1"
                     /translation="MGKEPSNKGNRLFKGISIQRQETRAAFLFLLPNLLGFGIFVVFA
                     VGMSFWLSFQDWNLLSKPIFVGLANYKKLFVADPDFMRALYNTVYFVIGIVPAGVVVS
                     LLLALLVNQKLKGIAFFRTAFYMPVVTPTIAIGLVWAWLYNADYGLINNFLSMIGITN
                     LPRWLASTIWAKPAIILMNIWHQAGYYMVIYLTALQNIPDQVYEAADIDGTNSWQRFI
                     HITLPLLSPTTFLVFVMKAMGSFKLFEESYIMTQGGPGGSTETIVYAIYKHGFEWFRM
                     GYAAAIAWVLFIIIFACTLIQFKLQKNWVHYE"
     gene            2168..3154
                     /locus_tag="VF531_09510"
     CDS             2168..3154
                     /locus_tag="VF531_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7714249.1"
                     /translation="MPAIRVSHLSKDFTYYEKELGFANSLSNLFHRRKLTKAAVSDIS
                     FEIEAGEIVGFLGPNGAGKTTTLKMLSGILFPSGGEAEVLGYVPWERQKGFKMQFSIV
                     MGQKNQLWWDLPANESLYLNKCIYEIPDAEYFSFLEELSELLEVKSLLKIQVRRLSLG
                     ERMKMELIAALIHKPRVILLDEPTIGLDLISQQKIREFLKYYNQQTKTTIMLTSHYLV
                     DIEELCKRTVVINQGRIVYDGELAGINQLFSAQKIIRLQFAEKIDREKLTGFGTVKEF
                     DGVSATLTVERTDVKALSLRILENLPVVDFNIEDVPLEEGIAALYNGGTVKG"
     gene            3210..>3539
                     /locus_tag="VF531_09515"
     CDS             3210..>3539
                     /locus_tag="VF531_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX7714250.1"
                     /translation="MMKKYLRSFLLGIQNAMEYRANFLLSMVSAAFPIFIQYFLWTAI
                     YRGDADQAISGFTYAQMIAYTFMASITSRLIRTGFEYEINDDIKNGGLNKFIVKPIDY
                     FSYRMSCF"
BASE COUNT         1044 a          847 c          772 g          876 t
ORIGIN      
        1 ttccggaaat cgagaaacct ttgacaaaat acttctgtcc gaagagaaac aggataatca
       61 ccggaatcaa agcaatgatc gttcctgcca gtaacacctc ccattcggta ttgaactccc
      121 cccggaaaaa agtcaatcca acttgaatcg gcattttttc aggagtgttg ataacaatca
      181 acggccacaa aaaattgttc cattgatctt tgaaggcaaa gatcgcaact gtagccaaag
      241 caggcttgga taacggcata atgatgcgga tgaatgtctg gaaccttgaa cagccgtcga
      301 tcttggcggc atcctccaac tcaccgggaa gggtcaggaa gtactgcctt aataaaaagg
      361 tacaaaagat cgtgaacatg ttaaaaactt caggcaaaat caatgcctta taactgtcaa
      421 tccagccgaa gaaacgcatc aggataaaat taggaataat caacacttgt cccgggatca
      481 tcatcgctcc caaataaaga agaaagagtg tatctcggcc tggaaaccgt aacctggcaa
      541 aggcatatgc cgccaatgat gagactataa gctgcattat tgtcacagtc gacgacatat
      601 acaggctgtt caataaaaac cggccaaacg gaaccatggt ccaggctcta acatagttgc
      661 tccaatttaa aactttaggt atccattggg gtggaaaagc ataaatctca ctgtgcgttt
      721 ttaatgaaga cgagagcgcc catgccaagg gcaccaacgt tattatcgcg atcaaggtca
      781 aaattaggta tgtaagaatc tgtttaccgt aaacaggttt ttttctggcc tttatctgat
      841 ataattctaa ttccattttg catccatctc cttttattca taatgcaccc aattcttttg
      901 gagtttaaac tgaatcaagg tgcaggcgaa aatgataata aagagtaccc aggcaatagc
      961 ggcggcgtat cccatccgga accattcgaa accatgtttg taaatggcat aaacaatggt
     1021 ttcggttgag cctccgggtc cgccttgagt catgatatag gattcctcaa agagcttaaa
     1081 agagcccatc gccttcatca caaaaaccaa aaaggttgtc ggcgaaagca aggggagagt
     1141 gatatggata aagcgctgcc aactgttagt cccgtcaata tcagccgctt cgtaaacctg
     1201 gtccggaata ttttgaagcg cggtaagata aatgaccata tagtatccgg cttgatgcca
     1261 aatgttcatc aaaataattg cgggtttggc ccatatcgta ctcgcaagcc atcgtggcag
     1321 attagttatc ccaatcatgg aaagaaaatt attgatcagt ccgtaatcgg cattatataa
     1381 ccaggcccaa accagaccga tggcaattgt cggagtgaca acaggcatat agaacgcagt
     1441 gcggaaaaaa gcaattcctt ttaacttctg attgaccaat aatgccaaaa gaagcgacac
     1501 cacaactccg gcaggaacaa tgccgatcac gaaatagacc gtattgtata acgccctcat
     1561 aaaatcgggg tctgccacaa ataacttttt atagtttgcc aatccaacga atatcggttt
     1621 gctaagcagg ttccagtctt gaaagcttaa ccagaaagac atccccactg caaaaaccac
     1681 aaaaatacca aatcccagca gattgggcag aagaaataaa aaagccgccc tggtctcctg
     1741 ccgttgaatt gagatccctt tgaacaaacg gtttccctta tttgacggtt ccttacccat
     1801 catcatcccc cctggttttt ccaaaagata tattgatata atggtatatt gatatatctt
     1861 ttattgacac aaaaatcaca taattaatta tacttgctat ccatcaaaaa atcaattgcc
     1921 tgattctttt atttttattt tgcattcaaa aaaaccgttc ccgcttgtga cgggtctgaa
     1981 ttgcctgctt cgctttttcc cttgcgggag actgagtatc accgggaccc gagagtgtgc
     2041 accccctggg gcacaacagc caataggagt gagccgcaag gattttacaa ttttctgtcg
     2101 aatcaatatt atctaaaagt gtcgtttaaa actctgttct cactataaaa cttaaggttg
     2161 gtaccccatg ccggcaatcc gcgtcagcca tctctccaag gactttacct attacgagaa
     2221 agaattaggg tttgcaaact ccctgtccaa tctctttcac cgccgcaagc ttaccaaagc
     2281 cgccgtatcc gacatctctt ttgagattga ggccggagag atcgtcggtt tcctgggccc
     2341 caacggagcc ggaaagacga ccaccctcaa gatgctctcg gggatcctct ttcccagcgg
     2401 cggagaggcg gaggttttgg ggtacgttcc ctgggaacgc caaaagggct tcaagatgca
     2461 attttccatc gttatgggac aaaaaaacca gttgtggtgg gacttacccg ctaatgaatc
     2521 cctgtatctg aataagtgca tctacgagat acctgacgcc gagtacttct cttttttgga
     2581 ggaactctcc gaactattgg aagtgaaaag tctgctcaag atccaggttc gccggctctc
     2641 gctcggagag cggatgaaaa tggagttgat tgcggcgctg atccacaagc cccgggtcat
     2701 cttattggat gaacccacca tcgggctgga tctcatctcc caacaaaaga tccgcgagtt
     2761 tttaaagtat tataatcaac agaccaagac caccatcatg ctgaccagcc attacctggt
     2821 ggatatcgag gaactctgca aacggaccgt agtcatcaat caaggccgga tcgtgtatga
     2881 cggcgagctc gccggtatca atcagttatt tagcgcccaa aagatcatcc ggctgcaatt
     2941 tgcggaaaag attgatcggg aaaaactaac cgggttcggc acagtgaagg agttcgacgg
     3001 ggtcagtgcc accctgacgg tggagcgaac ggatgtgaaa gcgctctccc tccggatcct
     3061 tgagaacctg ccggtggttg attttaatat cgaagatgtc ccgctggagg aagggattgc
     3121 tgcgctttat aacggcggca cggtgaaagg ctaagaagcc tatccgtccc ttaacggttg
     3181 aacccaacga aaccgctgga aaggcagcaa tgatgaaaaa atatctccgc tcttttttac
     3241 tgggaatcca aaacgccatg gagtaccggg ccaatttcct gctcagcatg gtcagtgcgg
     3301 cgttccccat ctttatccaa tactttttgt ggaccgccat ctaccggggt gatgcggacc
     3361 aggccatcag cggttttacc tacgcccaga tgatagccta taccttcatg gccagcatca
     3421 cctcaagact gatccggacc ggtttcgaat atgagatcaa cgatgatatc aaaaacggcg
     3481 gcttgaataa gtttatcgtc aaacccattg actatttcag ctaccggatg tcttgcttc
//
LOCUS       DASVMB010000335         6108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_117399,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000335 DASVMB010000000
VERSION     DASVMB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117399"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..940
                     /locus_tag="VF531_09520"
     CDS             179..940
                     /locus_tag="VF531_09520"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7714251.1"
                     /translation="MKKFTTIILGLIFVCGILSGVPIFGAESATDILRKLDYNQAPSS
                     VIYDVQMVIHQADRVDTKTMKLYGEGQEKSYALFLSPARDKGTAVLRLNDNLWMYLPS
                     AEKTMKISGHMLRQSFMGSDFSYSDSTERLQLLDLYDATVTGAAELDGRNVYLLELKA
                     KQADANYFTRKLWIDQERYVALRQEMYAKSGKLLKVMTTESVLKVGDRFFAAKVKMED
                     KLKKNTYSEMIFTDIKLDEAIPDSIFTIQNLEKKQ"
     gene            1278..2111
                     /locus_tag="VF531_09525"
     CDS             1278..2111
                     /locus_tag="VF531_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7714252.1"
                     /translation="MEPKEWSKLWHDPWLNLESFHAVCIRHGFPVHMHDYYVFCFIEK
                     GLQSFSYRKTKYVTPPGGLIILNPGDAHTGEPADEYGFEYRAIYPTISHMQEAMTELF
                     GRNHDFPLFPKVRIDDPELTGDIRALHLSLVHEASPLERESLFLTVLTKAIIRYAEIN
                     PVPQVSGKERHRVTQACNYIHAHATEGISLSQVAGYVNFSRYYFLRVFHEEVGMSPHA
                     YLESVRISMAKKLLEKGISLNQVAHESGFSDQSHFTNRFKRLVGITPGQYVREIRNEY
                     H"
     gene            2161..3090
                     /locus_tag="VF531_09530"
     CDS             2161..3090
                     /locus_tag="VF531_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7714253.1"
                     /translation="MMVKPSIQRYKIKILLQKESYFLAVVGAVLLWSISFVATKIAFK
                     AFPPLTLGALRFILAAWLLAILLWMQRGFIRPTRNDLGSLLMSGILGITMYFSMENYG
                     VKLATAADASLIVAAYPAITMVLEMVFYRVSISRVRFLGVGLAMFGLYLIIRESTNIG
                     GPYRLLGDVILAATGIVWSFYNFITRKIANKYPTILITFYQTLAGAVAFIPLALAERK
                     QWRMPGPDSLFALLYLGLFCSVAAFLLYAYGLRKMASSSAVTLMNLVPVFGVCFSVLL
                     LHESISIVQVLGGLIVIAGIILGIRQIKQDNED"
     gene            complement(3267..4091)
                     /locus_tag="VF531_09535"
     CDS             complement(3267..4091)
                     /locus_tag="VF531_09535"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7714254.1"
                     /translation="MEIIEIIKVIFLGFIEGITEWLPISSTGHMILADEFIKLQVTPA
                     FKEMFLVLIQLGAIMAVVILYFHKLNPLSPQKSVIEKQATISLWLKVIVACVPLGVIG
                     FLFNDLIDKLFYNYLTVAITLIFYGILFIIIENRNRKRESKINEFSQLNYKTAFLIGL
                     FQVLSVVPGTSRSGATIIGAILIGTSREVAAEFSFFLAIPAMFGASLYKIIKFGLGFT
                     GMEIAILVTGMVIAFVVSVLAIKFLMGYIKNHDFKAFGYYRIVLGAIVLLYFLFIA"
     gene            complement(4107..4847)
                     /locus_tag="VF531_09540"
     CDS             complement(4107..4847)
                     /locus_tag="VF531_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714255.1"
                     /translation="MVDLLYCEILKLKRSKMFLFSIMGAAAAPVMCFIGCIKSKLQYY
                     PDGIITFGKMFSETNLYTLVLVGVTLYGVITAYLFSREYTENTLKSILTIPVAKTKFF
                     VGKHIVLFLWIMALTLISWLLTFILGVIVQLEGLDLRILFDSLGHYLSGGFLVYLLTT
                     PFVLITLWFKNLVPTIIFSVIITMGNAAVLNDVDFVSLYPWSATYVIASGEVVTKYPL
                     VDSYIVVFALSIIGFAASLAYFRKMDIR"
     gene            complement(4840..5763)
                     /locus_tag="VF531_09545"
     CDS             complement(4840..5763)
                     /locus_tag="VF531_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714256.1"
                     /translation="MSPYAVETYALTKQFGEELGVDNLSIHVEQGKIYGLLGRNGAGK
                     TTTMRMIMGLVRPTAGEIKIFGRDYHQDVEQTYSRIGSIIETPGFYENLTGSENLKIL
                     ARLRGIHREDEIHKALVTVGLDKEPRKVFGDYSLGMKQRLGIAAAIMHQPELLILDEP
                     TNGLDPIGISEVRHFLMNLCRERQTTIMISSHILSEIEQMAETIGVMHQGRLIEEIQM
                     EDLRKRKRQYVEFEVSDENRATLLLEDKLKLMDYSVHEGHTLRIYSHFNRRAEINQLF
                     VENGIGVSKITISEEKLEDYFAGLIGGGKIG"
     gene            complement(5876..>6108)
                     /locus_tag="VF531_09550"
     CDS             complement(5876..>6108)
                     /locus_tag="VF531_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7714257.1"
                     /translation="TRINISDNGQGIPESKLPFVFERLYKCDDARTGQGSGLGLAITK
                     ELVEAHHGTIVATSIPHKKTTFTITLPIKSSV"
BASE COUNT         1678 a         1430 c         1375 g         1625 t
ORIGIN      
        1 aatcaacagc atgatttacg gagttttcga tgggggaatc ttcttgaaag gaacgattct
       61 ggggttggct gccgccgtcc tggcttccat cttccccgct ttgagcgggg tccggctcaa
      121 acccaccgaa gcgttacgga aagaataaaa attaataaac gaaaggagta tcacgggtat
      181 gaaaaagttc accactatca ttctcggatt gatattcgtc tgcggcatct tatcgggagt
      241 ccctattttc ggggccgaat cggctacgga tatcctccgg aagcttgatt acaaccaagc
      301 accttccagc gtgatttacg atgtgcaaat ggtgattcac caagccgacc gggtcgatac
      361 caaaaccatg aagctatacg gggaaggcca ggaaaagtct tacgcccttt tcttgagccc
      421 ggcgcgggac aaaggaaccg cagtgctccg cttgaatgat aatctttgga tgtacctgcc
      481 ttccgccgaa aagaccatga agatctccgg ccatatgctg cggcaaagct tcatggggag
      541 cgacttctcc tactccgaca gcaccgagcg gctccaactg ctcgacctgt acgatgcgac
      601 cgtcaccggc gccgcggaac tggacggcag aaatgtctat ctcctggaac taaaggccaa
      661 acaggcggat gccaactatt tcaccaggaa actctggatc gaccaggaac gttacgtcgc
      721 cctgcggcag gagatgtacg ccaaaagcgg caaattgctc aaggtgatga ccaccgagag
      781 cgtcctgaag gtcggcgacc ggttctttgc cgccaaagtc aagatggagg ataaactgaa
      841 aaaaaacacc tactccgaaa tgatcttcac cgatatcaag ctggacgaag ccattccgga
      901 ttcgatcttt accattcaaa acctggagaa gaagcaataa ggcgtgagta acgcgtttca
      961 tgaaacgcga agcaacaggg aactcgcgcc ccgtggggcg cgcggcaata ggcaacagga
     1021 gtattaaggc acgaggcatg aggcgtgaga tgcagataga gaaataagat tataacccct
     1081 tccggaaatt tcgggagggg tttttaggtt catccggttt gttgttcaac caattccggg
     1141 tacattcaca aatgggcaat ttttttcaag acgtttcatt gactcgaagt tataataaaa
     1201 ctaaaatgta tctaactatt gaatatcgcc agacttgcat gactttttaa atatcgttat
     1261 atacaaggga gcagcaaatg gaaccaaagg aatggagtaa attatggcat gacccctggc
     1321 taaacttgga gtcgttccat gccgtctgta tcaggcacgg gtttcccgtt catatgcatg
     1381 attattatgt attctgtttc attgagaagg gattgcagtc tttttcatac cggaaaacga
     1441 agtatgtcac gccaccgggc ggtttgatta tattgaatcc gggagatgct cacaccggag
     1501 aacccgcgga tgaatatggg tttgaatatc gcgcaattta cccaaccata tcacacatgc
     1561 aggaagcgat gacggaactg ttcggccgaa accatgattt tcctcttttt cccaaggtaa
     1621 gaatcgatga tcccgagctg accggtgata ttcgtgcgct acacctgtcg ctggtccatg
     1681 aggcctctcc cctggaacgg gagtccttgt ttttgacggt tttaaccaaa gccatcatcc
     1741 gctatgccga aataaatccg gtgcctcaag tttccggaaa agaacggcac cgcgtgacac
     1801 aagcctgtaa ttatattcac gctcatgcaa ccgaggggat ttccctatcc caggttgccg
     1861 gatatgttaa cttcagccgc tattactttt tacgcgtctt tcatgaagag gtcggaatgt
     1921 ctccccacgc ttatctggaa agtgtgcgga tcagtatggc aaagaagctt ctggaaaagg
     1981 ggatatcttt gaatcaggtt gcccatgaat ccggtttcag cgatcagagt cattttacaa
     2041 accgtttcaa acgtttggtg gggatcacgc cggggcaata tgtccgggag atccgaaatg
     2101 aatatcactg aaagaactct tcttccaaga ataaaaatga aatcgatgag gagaacccgg
     2161 atgatggtaa agccaagcat acaaagatat aagattaaga ttcttttgca gaaagagtct
     2221 tactttttag cagttgttgg agcggttctc ctttggagta tctctttcgt tgccacgaaa
     2281 atagcattca aggcatttcc gcctttaact ctgggcgcct tacgttttat tttggcagca
     2341 tggttactcg caatcctttt atggatgcaa cgcgggttca tccggccgac acgcaatgac
     2401 cttggcagtc tcctgatgag cggtatcctt ggtatcacga tgtatttttc gatggaaaac
     2461 tacggagtaa aacttgccac ggcagcggat gcttccctga ttgtagcggc atatccggcc
     2521 ataaccatgg ttctggaaat ggtattttac cgtgtttcaa tttcccgggt tcgtttttta
     2581 ggggtgggac tggcaatgtt cggtttgtac ttaatcatcc gtgaaagcac aaatatcggg
     2641 gggccgtacc gtttattggg cgatgtcatt ttagcggcaa ccggaatcgt ttggtctttt
     2701 tacaacttta ttacccggaa gatagccaat aaatacccga cgatcctgat taccttttat
     2761 cagacgctgg ccggcgctgt cgcatttata ccgctggcgc tggctgaacg caaacagtgg
     2821 cgcatgcccg ggccggattc cctttttgcc ctattgtact tgggattgtt ttgctcggtt
     2881 gccgccttcc tattatatgc ctatggctta cggaagatgg catcaagttc ggcggtaacc
     2941 ctgatgaacc tggtgccggt attcggcgtt tgcttttccg ttttgctgct ccatgagtcg
     3001 ataagtattg tccaagtatt gggcgggttg atcgtgattg ccggaatcat ccttgggatt
     3061 cgtcagataa agcaggataa tgaagattaa attctatata agtagctcct agataataaa
     3121 aagattaaaa accccttccg ggaatttttc gggaggggtt tttgggttca tccggcattg
     3181 tttgttcaac caatattgac ggtatattta caaataggta tttttctatg cgggtcttac
     3241 gcctcaaagc tggaataagt cacgtctcag gcgatgaaca aaaaatataa caaaacgata
     3301 gcgcccaaaa caattcggta atagccaaag gccttgaagt catgattctt aatgtaaccc
     3361 attaaaaact tgattgccaa caccgaaacc acaaaagcaa taaccattcc ggtcaccagt
     3421 atagcgatct ccatccctgt aaacccaaga ccaaatttaa tgatcttgta aagactggcc
     3481 ccgaacatgg cgggaatagc caggaagaat gaaaactccg ccgccacttc ccgggatgtg
     3541 ccgatcagta tcgccccgat aatggtcgcc ccggatcggg atgtaccggg caccaccgac
     3601 aacacttgaa agagcccgat cagaaaggct gttttatagt taagctgaga aaactcattt
     3661 atctttgact ctcgcttccg attcctgttt tcgattatga taaacaaaat tccataaaaa
     3721 ataagcgtaa tcgccacagt caagtaatta taaaacagct tatcaatgag gtcattaaac
     3781 agaaacccga tgacccctaa gggaacacag gccactatca ctttcagcca taacgaaatg
     3841 gtcgcttgct tctcaatcac cgatttttgc ggtgataacg gattgagctt atggaagtat
     3901 aaaatgacga ccgccataat ggccccaagt tgaattaaga ctaaaaacat ttctttaaaa
     3961 gccggggtta cttgtaattt gataaactcg tccgccaaga tcatatgacc ggtactgctg
     4021 atcggcagcc attcggtaat gccctcgata aaacctaaga agattacctt gatgatttca
     4081 ataatttcca tgagcttctc ctccaattaa cgaatatcca tctttctaaa ataagccaaa
     4141 ctagcagcaa atccaatgat ggacagagcg aatactacga tataagaatc gaccaaagga
     4201 tatttggtta ctacttcacc actggcaatg acataggtcg cggaccaagg gtataaagag
     4261 acgaagtcca catcgttgag tacggctgca tttcccatgg taatgatgac tgagaagata
     4321 atcgtaggga ccaggttttt aaaccataaa gtgatcagga caaatggagt tgtcagtaaa
     4381 tataccaaaa atccgccgct cagataatgc cccaatgaat caaagaggat tcttaggtca
     4441 agtccctcaa gttgtacgat aaccccgagg ataaaagtga gtaaccatga gatcaaggtc
     4501 aatgccatga tccataagaa taatacgata tgtttgccaa caaaaaattt ggtcttggcc
     4561 actggaattg tcaaaatgga ttttaaagta ttttcggtat attcccggct gaagagatag
     4621 gctgtgataa ctccataaag agttaccccc accaagacca gcgtatatag gttggtttcg
     4681 gaaaacattt taccaaacgt gatgatccca tccggataat attgaagctt ggacttgata
     4741 cacccgataa aacacatgac cggggctgcc gcagccccca taatactgaa aagaaacatc
     4801 ttggaacgtt ttagtttcaa tatctcacag tagagtagat caaccaattt ttccaccccc
     4861 gatcaggcct gcaaaataat cttccaactt ctcttcactg atagtgattt tggagactcc
     4921 aatcccattt tccacaaata attgattaat ctccgcccgt ctgttgaaat gggagtagat
     4981 ccggagcgta tgaccttcat ggacgctata atccattaac ttcagtttat cctccaagag
     5041 caaagtggcc cggttttcat ctgagacctc aaactcaaca tattggcgct tcctttttct
     5101 caggtcctcc atttggatct cttcaatcaa tctcccttga tgcatgactc cgattgtctc
     5161 ggccatctgt tcaatttcgc ttaaaatatg gctggagatc atgatggtgg tctgccgctc
     5221 gcggcagaga ttcatcagaa aatgacgtac ctcactgata ccaatgggat ccaacccgtt
     5281 tgtaggctcg tccaaaataa gcaactccgg ctgatgcata atggctgcgg cgatccctaa
     5341 ccgttgtttc attcccagag aatagtcgcc gaacacttta cggggttctt tatcaagacc
     5401 taccgtaact aaggccttgt ggatctcatc ctcgcgatgt atgccccgga gccgcgccag
     5461 gattttaaga ttttcactgc cggtgaggtt ttcataaaac ccgggagttt cgatgatgga
     5521 gccgatccgg ctataggtct gctctacatc ttgatgataa tctctgccaa aaatcttgat
     5581 ttctcctgcc gtgggacgaa ccaggcccat gatcattctc atggtcgttg tcttgccggc
     5641 tccgtttcta cccagtaagc cgtagatctt tccttgttcc acatgaatac tgagattatc
     5701 tacgcccaat tcctcgccga actgtttggt tagggcatag gtttcaactg cataggggct
     5761 caactgtttc tcctccttgg caaaaagtct atctttattt tagaacccat tacttacctg
     5821 ccccttactc agatcttact ttttccttac ttgaactcac aaaaaaagcc ctgtttcata
     5881 cagagctctt tatcggtagg gtaatggtga aagttgtttt tttatgtgga atacttgtcg
     5941 cgacgatggt tccgtgatgg gcttcgacca gctccttggt gatagccagc cccaggccgc
     6001 ttccctggcc ggtacgggcg tcatcgcatt tataaagcct ctcaaagaca aaagggagtt
     6061 tcgactcagg tattccttga ccgttatcgg agatattgat cctggtgt
//
LOCUS       DASVMB010000336         5801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_483276,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000336 DASVMB010000000
VERSION     DASVMB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483276"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF531_09555"
     CDS             <1..410
                     /locus_tag="VF531_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714258.1"
                     /translation="SYKTASGKLVSQPFAKAWYLTLNKNSKNKKEAVQWISYFVGEEA
                     SQIAIKDGANPSSVKVWSAPQTADIRKDSAIFLKTFKMARMTPNLAELPAIEDKLGQA
                     LTFVLANKKTAESALNDVNEEAVKILKQSGRLK"
     gene            572..1894
                     /locus_tag="VF531_09560"
     CDS             572..1894
                     /locus_tag="VF531_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7714259.1"
                     /translation="MKETSKYDVIVVGGGTAGLFAAISSANAGAKTLLIEQKDYLGGL
                     LTAGMGIGGVFDIKGTQVISGAIPELIRRISDTKGGCGIVKIDNQDRWIPSVISIDPE
                     WMKQIAFDMVQESGCQLLLRTAFMDSTTENNYVKGINVIYAGQKISLFGDVIIDATGN
                     ADVAASAGAETMIGDEHGEHQSQSCVIRISNVNTDEFVAYMNNTINTNDRAEWTIEEA
                     ACRGGGDGYWLPWKDASGVHLPNTCGIYYHGNKGDIFINATHIEIDPLDPFAVAAALV
                     ELRKQANDICAFLKSKVPGFKDAYLSNVYEFGIRDSRRVVGEYIMTLQDLEKNRHFED
                     VVCRGAYPPDIHKNYGDVKINTTGCFNYEIPYRAIISKDFDNLLMAGRCISATFEAAS
                     GIRGMGPCIATGQAAGVAAALAVSNKDTPKKLNTQQVQQALRLQGVIL"
     gene            2172..2984
                     /locus_tag="VF531_09565"
     CDS             2172..2984
                     /locus_tag="VF531_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7714260.1"
                     /translation="MEPVKRNNGAKSGNSVQVLERCFAIMEAIAANQGEASLATLGKS
                     LSIPHSTIHRILASLIKLGYVEQNQENGYYQLGLKILSLSNTILEKLDLRRVSKAFLE
                     ELMQRTGETANLVVLDGDEVVYIEKVESHASVRVFSLIGRRAPVHVTGAGKILLSEMA
                     QPDIIDILQRKGMPKMTSHSITSLSKFLDELALVRKQEFAMDNEECELGARCIAAPVR
                     NHLGRIIASISVSAPVSRFSLERVKELTPVVKEVGLKLSKELGFSEDMLKLG"
     gene            3062..4342
                     /locus_tag="VF531_09570"
     CDS             3062..4342
                     /locus_tag="VF531_09570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7714261.1"
                     /translation="MELQEKASKLLKEFKGENYAFGLGVIDQVGEFASRYGKTALVIG
                     NEKYLRPVIDKVEASLNAHGVQPAGGRVVPDAGPNAPREDVYRIESYILHYQPDCIIP
                     IGGGSTIDAGKAADLLATLGEYSPEIDSYFGTGLVTETLKKTGKKLKPLIAVQTAASS
                     GAHLTKYSNITDPVAGQKKLIVDEAIVPAKAVFDYAVTESMPVGVTIDGALDAVAHCV
                     EVFFGADAVKFELLREVAECGITLVVQNAGKVIKNPHDLEARKALGLAADLGGYSIMI
                     GGTNGAHLTSFSLVDLTSHGRACGIMNPYYTVFFAPSVERQLRVVGDIFKQAGFITSD
                     LNGLKGRELGIAVARGMVAFNQSIGSPTKLSDLPGFGEEHINRAMAAAKDPQLEMKLR
                     NMPVPLTASQVDQYMLPILKAAQSGDFSLIKNMA"
     gene            4456..>5801
                     /gene="larA"
                     /locus_tag="VF531_09575"
     CDS             4456..>5801
                     /gene="larA"
                     /locus_tag="VF531_09575"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX7714262.1"
                     /translation="MKTGIMNLGTESLVIDLPDQTDILALNEAVPLENMADAIQAALN
                     HPIGTPGLDQIIGSKLQTNPAARAVVVVSDNTRPVPYKGESGILWPVIRRLLEHGFTK
                     ERILVLVANGTHRALAEPELRSMLDPRVFEKGIQVKNHNCRDHTHLVNLGKTSRGSQI
                     HIDSDYMAADLKILTGLVESHFMAGASGGRKSVCPGLIGEEGTYVFHGAPMLASKNAR
                     DLQLENNPCHEEALEIARKAGVDFIINVTLDRDFRITGVFAGDLEKAHQSAVERLQKD
                     SCIQISHEYDIVVTHAGFVGVNHYQTAKAGVAAIPALKSNGKLILVANNTDPNPIGSP
                     MYHTVLGLLTLIGADKFKQLLVSPDWSFIPDQWQVQMWAKLFNKIPMDNFVYYSPQLS
                     QSDYRLLPGQDGNRYLPPASRYHGSLKTVPVMIHNSVQQFTTELTNEGKKEITVAYL"
BASE COUNT         1631 a         1227 c         1490 g         1453 t
ORIGIN      
        1 caagctataa gaccgcttcc gggaaactag tatctcaacc attcgccaaa gcatggtatc
       61 tgaccctcaa taaaaacagt aaaaataaaa aagaggcagt acaatggata agttattttg
      121 tgggggaaga agccagccaa atcgcgatta aggatggagc caatccaagc agcgtaaagg
      181 tttggtctgc tcctcaaacg gctgatattc gaaaagattc ggctattttt ctgaaaacat
      241 tcaaaatggc cagaatgact ccgaatctag ccgaactgcc ggccattgaa gataagttag
      301 gccaggcact tacctttgta cttgccaata aaaagaccgc tgaaagtgct ttaaatgatg
      361 tcaatgaaga agcagttaaa atactaaaac agagcggacg tcttaaataa taagtagttt
      421 caagcaatta ccttaaaaca cagaccggga ctatagtgac tgaaagtgcc tgcatttaag
      481 ccatatttgg acattttttg aattatagtc ccggctttta ttattatgtt ttgatagaca
      541 gggcgactga gttattgaga gggtgaattg aatgaaagaa acatcgaagt atgatgtgat
      601 tgtagtcggg ggaggaactg ccggattatt tgctgcaatc agctctgcaa atgcgggagc
      661 aaaaaccctt cttattgagc aaaaagatta tttgggcgga ttattgacag cgggaatggg
      721 aattggggga gtattcgata taaagggtac ccaagttatt tccggagcaa ttcccgaact
      781 tatccggagg atctctgata ctaaaggcgg atgcggcatt gtcaaaattg acaaccaaga
      841 tcgatggatt ccaagcgtga tatcgattga tccggaatgg atgaagcaga ttgcttttga
      901 tatggttcaa gagtcgggat gccaactttt gctacggacc gcttttatgg attcgacaac
      961 cgaaaataac tacgtaaaag gaatcaatgt aatttatgca ggtcaaaaga tcagtttatt
     1021 tggcgatgta attatcgatg ccacaggtaa tgccgatgtt gccgcatccg ccggagcaga
     1081 aacgatgata ggtgacgaac acggtgagca tcaatcacaa tcttgtgtca ttcggataag
     1141 taatgtaaat accgatgaat ttgttgccta tatgaacaac actatcaata caaacgacag
     1201 agcagaatgg acaattgaag aagcagcctg ccgcggcgga ggcgatggat attggcttcc
     1261 ttggaaagac gcttcaggcg ttcatcttcc taatacctgc ggtatttatt atcatggaaa
     1321 caagggtgat atttttatca atgcaactca tatcgaaatc gatccgttgg acccgtttgc
     1381 cgtggccgct gccttggttg aacttagaaa acaagccaat gacatttgcg cctttttaaa
     1441 aagcaaagtg ccgggtttta aagatgctta tctttcgaat gtttatgaat ttgggattcg
     1501 cgacagccgg agagtagtgg gagagtatat aatgactctt caggatttgg aaaaaaaccg
     1561 acactttgag gatgttgtat gtcgcggcgc ttatccgccc gacattcaca agaactatgg
     1621 agacgttaaa ataaacacca ccggttgttt caactatgag attccttata gagccatcat
     1681 ttccaaagat tttgacaatt tactcatggc aggcagatgc atatctgcaa cttttgaagc
     1741 tgcctccggt attaggggga tgggaccttg catagcaacc ggccaagcag cgggggtcgc
     1801 tgccgccctg gcggtatcta ataaagatac acccaaaaaa ttgaatactc aacaagtgca
     1861 gcaagctttg cggttacagg gcgtcatatt ataaaaacat gaaattgaaa atattcttcg
     1921 gttgagatga gaagggtata accgaatgga gtcccgcttt gctccaaaga tgcacctgta
     1981 aaaggtagac gttaaatgct ttggattttt gccattgttt gttgacgtat atattataaa
     2041 tggtaatata taattaatct cgagaatttt gaaaaataac tgccctgaat ttttatcaaa
     2101 accgtaagat tatatttata agcgtttcaa tttatttgat ttgttggaat ttgattttgg
     2161 gaggatgaaa aatggagccg gttaaaagga ataacggcgc aaaatccggt aattcggttc
     2221 aagtattgga acggtgtttt gcaattatgg aagcgatcgc cgccaatcaa ggggaggctt
     2281 ccttagcaac cttaggcaaa agtttatcca tcccccatag tacaatccat cgcatactgg
     2341 ccagcctaat caaactgggc tatgttgagc aaaaccagga aaacggctac taccagctgg
     2401 ggttgaaaat tctctcgctt tccaatacga ttttggaaaa attggattta cgcagggttt
     2461 ccaaggcgtt tttggaggaa ttgatgcaga gaacgggtga gaccgccaat ctggtagtgc
     2521 tcgacgggga tgaagtggtt tacatagaga aagtggaaag ccatgcttca gtacgggtct
     2581 tttctttgat cggcaggcgc gccccggttc atgtcaccgg cgcgggaaag atactgctgt
     2641 cggagatggc tcaacccgat attatcgata tcttacaacg taaagggatg ccaaagatga
     2701 cctctcattc gattaccagt ctctcgaaat ttctggatga actggcttta gtccgtaaac
     2761 aggaatttgc catggacaat gaagaatgcg aattgggagc cagatgtatt gccgcaccgg
     2821 ttcgcaatca tctgggaagg atcatcgctt cgataagtgt ttccgcgccg gtttcccggt
     2881 tctctttaga gcgtgtcaag gaattaactc ccgtagttaa ggaagttggc ttgaaattat
     2941 ccaaagagct gggattctca gaggatatgt taaagcttgg gtaacttacg cgcaaaaaat
     3001 gttatgccgc tctatagatc catggaaatg aaggatattt ctaaaaagga gccgattgat
     3061 tatggaactg caggaaaaag cttcaaaact cctgaaggaa tttaaggggg aaaattatgc
     3121 cttcggcttg ggagtcatcg accaggtggg cgagtttgcc tccagatacg ggaagacggc
     3181 gctggttatt ggaaatgaga aatatttaag accggtcatt gacaaggtgg aagcctcctt
     3241 aaacgcccat ggagtacaac cggcgggcgg ccgggtcgtg cccgacgccg ggccgaatgc
     3301 tccgcgggag gatgtttaca ggattgagag ctacattctt cattatcaac cggattgcat
     3361 cattccaatc ggcggtggaa gcaccatcga tgcgggaaaa gccgcagacc tccttgcaac
     3421 gcttggagag tatagcccgg agatcgatag ctatttcgga accggtttgg tgaccgagac
     3481 cttgaaaaag accgggaaaa agctgaagcc cttaatcgct gtacagactg cggctagttc
     3541 cggagcccat ctgaccaagt attccaatat taccgatccg gtggcgggcc aaaaaaaact
     3601 cattgtcgat gaggccattg ttccggctaa agcagtattt gattatgcag tcaccgagag
     3661 tatgccggta ggggtgacca tcgatggcgc actggatgcg gttgcccatt gtgtcgaagt
     3721 cttttttggg gccgacgccg ttaaatttga gctccttagg gaagtggccg agtgcggtat
     3781 tacgctggta gttcaaaatg ccggaaaagt aatcaagaac ccgcatgacc ttgaggccag
     3841 gaaagcactg ggcctggccg cggatttggg tggatactcg atcatgatcg gcgggaccaa
     3901 cggcgcccat ttgaccagct tctccctggt ggatcttacc agccacggcc gggcttgcgg
     3961 aatcatgaac ccgtattaca cagtattttt cgccccgagt gtcgaacgac aattgcgggt
     4021 agtgggcgat atctttaagc aagccggatt tatcacttcc gatctcaatg gattaaaggg
     4081 ccgggaactc ggcatcgccg ttgcccgggg aatggtggcc tttaatcaaa gcatcggttc
     4141 gcccaccaag ctttcggatt taccgggatt cggtgaggag catatcaacc gggccatggc
     4201 cgcggccaag gacccccagt tggaaatgaa gcttagaaac atgccggtac cgctcaccgc
     4261 ctcccaggtg gatcaatata tgttgccgat cttgaaagcg gctcaaagcg gtgacttcag
     4321 tctgatcaag aatatggcct gagttaatcg gaaaaagttc tttgaaaagt gtgaatgcag
     4381 gacctccttg gtacatgagg gggtcctgcc gttattctga ttggacacga ctatcgtttt
     4441 taatggagga gcgatatgaa gacgggaata atgaacttgg gaaccgagtc tctcgtgatt
     4501 gaccttcccg atcaaaccga tatactcgcc ctgaatgagg ccgtcccgct tgaaaacatg
     4561 gcggatgcaa ttcaagcggc gttaaatcat cccatcggta ctcccggctt ggaccagatt
     4621 atcgggagta aattacaaac caatccggct gcccgggcag tggtggttgt ttccgataat
     4681 acccggccgg taccgtataa aggcgaatca gggatccttt ggccggtgat ccggcgacta
     4741 ttggagcatg gttttacgaa agagcgaatc ctcgttttgg tagccaacgg tacccatcgg
     4801 gccttggctg aaccggaatt gcggagtatg cttgatccaa gggtatttga gaaggggatt
     4861 caagttaaaa atcataactg ccgtgaccat acgcatctgg tcaatttagg caaaaccagc
     4921 cggggaagcc agattcatat cgatagtgat tatatggcgg cggatctcaa aattttaacc
     4981 ggtttggtgg aaagccattt catggccggc gcttccggag gcagaaaatc ggtttgcccc
     5041 gggttgattg gtgaagaggg aacctatgtg tttcacggcg cacccatgct ggcttctaaa
     5101 aatgcccggg atctgcaatt ggagaacaac ccctgtcatg aggaagctct cgagatagcg
     5161 aggaaagcag gggtcgactt tatcatcaac gtcacgctgg atcgtgattt ccgaatcaca
     5221 ggggtcttcg ccggggattt agagaaggcg caccaatcgg cggtggaacg tctacaaaag
     5281 gatagctgta tccaaattag ccacgaatat gatattgtag taactcatgc cggttttgtg
     5341 ggcgtcaatc attatcagac agccaaggcc ggggtggcgg cgattcccgc actaaaatcc
     5401 aacggcaaac tgattctggt ggcaaacaat accgatccca acccgatcgg cagcccgatg
     5461 taccacacgg tgttaggcct cttgacactg attggggcgg acaagtttaa acagcttctc
     5521 gtctcacccg attggagttt catccccgat cagtggcaag tgcaaatgtg ggctaaattg
     5581 ttcaataaaa tcccaatgga taacttcgtt tattatagtc cgcaattgtc tcagtctgat
     5641 taccggttgt taccggggca ggacggcaac cgatacctgc cgccggcctc gcgctatcac
     5701 ggctccttga aaacggtgcc ggtaatgatt cataattcag tacaacaatt cactactgag
     5761 cttacgaatg aggggaaaaa ggagattacc gtcgcatacc t
//
LOCUS       DASVMB010000337         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_631202,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000337 DASVMB010000000
VERSION     DASVMB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631202"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VF531_09580"
     CDS             complement(<1..119)
                     /locus_tag="VF531_09580"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7714263.1"
                     /translation="MRIGVDYYPEHWEESRWPVDAKLMREAGINLVRLAEFSW"
     gene            complement(159..1262)
                     /locus_tag="VF531_09585"
     CDS             complement(159..1262)
                     /locus_tag="VF531_09585"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX7714264.1"
                     /translation="MDLNSLRLEISSFLGFQTPGGSVSYEVVKTVNQPEYTRSQIKYQ
                     SLEGDEISAFLLIPKGQGPFPGVIVHHQHNQEWHLGKSEVCGIIGESLQSFGHGLANK
                     GMVVLAPDTIGFEDRRRSCSGVKPEPSDWLQYYNEMSYRMVVGDTLIRKILLDAALGV
                     SLLTALPEVDANRIGALGHSMGGNTVLFQTALDERIRFACASGAACSYKNKLIGGTGI
                     EMAEVIPGFAKRFDIDDLVKCIAPRRILLVSATEDRYSRDAVFITETASQAFEALEVP
                     SHIRHERFQGGHSMTRERFKFILDWMVAAGGLGSEEEENFPEKPAATESGPASTPAEL
                     PKPAEDSSQVSKPATPLPSDIPSNRTGGDVHHE"
     gene            complement(1264..2028)
                     /locus_tag="VF531_09590"
     CDS             complement(1264..2028)
                     /locus_tag="VF531_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7714265.1"
                     /translation="MQQAVELAVEGKILRGMYHLPEPATVRGPLVILFHGFTGNKLEP
                     HRIFLKLSRRLADQGVAALRFDFSGSGESDGDFEEMTFFSEIKEAGAILDYARSLSQV
                     DPSRIGLVGLSMGGAVAATLAGERATDIKALSLWSAATDSYMRQLMIPRLPTLLQDER
                     GCHDWDGNLVHPDFLDEMGRWNPVTTVSKFQGPVLILHGSRDETIPQEAALKYYRALP
                     GRARLKMIDDADHTYNRHDWERTVLDQTGAFFKETL"
BASE COUNT          511 a          676 c          564 g          514 t
ORIGIN      
        1 caccaagaaa attcggccag ccgcaccaga ttgattccgg cctcccgcat caacttggcg
       61 tctaccggcc aacggctctc ttcccaatgc tccgggtagt aatccacccc gattcgcatg
      121 tctcaatccc tccggtttga tggtaattcg ctcattgatt attcgtgatg aacgtcgcca
      181 ccggttcgat tgcttggaat atcagatggt agaggggtag cgggtttcga cacttgagag
      241 gaatcttcgg ccggtttggg caattctgcc ggcgttgatg ccggccccga ttcggttgca
      301 gcaggttttt cgggaaaatt ctcctcttct tcggaaccca agcccccggc ggcaaccatc
      361 caatcgagga tgaacttaaa cctttcccgg gtcattgaat gtccgccttg aaaacgctca
      421 tgccgtatat gggatgggac ttccagggct tcaaaggcct gactggctgt ttcagtgatg
      481 aagacggcgt cccgtgaata tctgtcttcc gtagccgata ccaataatat tctccgcggg
      541 gcgatgcatt ttaccaggtc atcgatgtcg aaacgtttgg cgaaacccgg gatcacttcc
      601 gccatttcga taccggtgcc gccgattagc ttattcttat aactacaagc ggccccgctg
      661 gcacaggcaa agcggatccg ttcatcaagg gcggtttgaa acagaactgt gtttccgccc
      721 atggaatggc ccagtgcccc gatacggttc gcatccacct ccggaagcgc cgtcaggagt
      781 gaaactccca aggccgcatc gagcaaaatc ttacggatca gggtatcgcc aaccaccatt
      841 ctgtaggaca tctcgttata gtactgcaac caatccgatg gttccggttt cacccccgaa
      901 caactgcggc gccgatcctc gaacccgatg gtatccgggg ccaggaccac catgccctta
      961 ttggccaaac catggccgaa ggattgcagc gattccccga tgataccgca gacttcgctc
     1021 tttcccaggt gccactcttg attatgttga tggtggacga tcacaccggg aaatgggcct
     1081 tggcctttgg gaatcaataa aaaggccgag atctcatccc cctccaggct ctgatacttg
     1141 atctggctcc gggtgtattc cggttggttt acggttttga ccacctcata agatacggag
     1201 ccgccgggag tttgaaaccc caaaaaactg ctgatttcaa gacgtaagct gttgagatcc
     1261 atgtcacaga gtctccttaa agaacgcgcc ggtttgatcg agcaccgtgc gctcccagtc
     1321 atgacggtta tatgtgtgat cggcatcatc gatcatcttt aaccgggccc ttccgggtaa
     1381 cgcccggtaa tactttaacg ccgcttcctg agggatcgtt tcatcgcggg atccatgaag
     1441 gatcaaaacc gggccttgaa acttggatac cgtagtcacc gggttccatc gccccatctc
     1501 atccagaaaa tcgggatgaa ccagattgcc atcccagtca tgacaacccc gttcatcctg
     1561 aagcagggtg ggaagacgcg gaatcatcaa ttgcctcatg taggaatcgg tcgcggcgga
     1621 ccataaggat agcgccttga tatccgtagc ccgttctccc gcgagggtgg ctgccactgc
     1681 ccctcccatg ctcaatccga ccaggccgat ccgggacgga tcgacctgcg acagactcct
     1741 ggcataatcg aggatcgcac ccgcctcctt gatctcactg aagaaggtca tctcctcaaa
     1801 atccccgtca ctctcaccgg agccgctgaa gtcgaagcga agcgcggcga cgccttgatc
     1861 ggcaagcctg cggcttaact tcaagaagat ccggtgtggc tccaacttgt tgccggtaaa
     1921 gccgtgaaat aagattacca acggcccgcg gaccgttgcc ggttccggca ggtgatacat
     1981 tccccggagg attttaccct ccaccgccaa ctccaccgct tgctgcatag gatcactcca
     2041 ttcactgatt cttaattctt atcaacgctc cccaacagca gtgcattctt aggacaagga
     2101 acgaccgagc aaggggctca tcccagaccc tcttgaaact ggatcttgca taaccgggcg
     2161 tacaaaccat tccgtgccaa aagctcctca tgccgtccgg cctcggcgat ccctcgatcg
     2221 tgcagtacca aaatttgatc cgcctctcga acggtggaga gccgg
//
LOCUS       DASVMB010000338         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_117824,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000338 DASVMB010000000
VERSION     DASVMB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117824"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VF531_09595"
     CDS             <1..855
                     /locus_tag="VF531_09595"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7714266.1"
                     /translation="EGLGYYRRVRLLHQGAEEVLTRYGGRLPADPEALAALPGVGPYM
                     AGALASIAFNLPVPAVDGNVIRVVTRVLAWNEPATTAHSHQVIRTWVAERFPDGAARE
                     FTQALMELGALICLPRNPYCRECPVRDECRGMQGNPEQYPVKQAARTIPAQRRIVLRI
                     NWKGRRLLVQRPSSGLLAGFWEYPNLQVETGVDAMVFARRWAEEHLGQTLQFIPFKTV
                     TQIFTHLRWDLEVLTAEWPVAGEPPQVPRGQWFPYEEEVELPRVTYLRHLEGDLSLKP
                     AGKKIIPE"
     gene            complement(944..1261)
                     /locus_tag="VF531_09600"
     CDS             complement(944..1261)
                     /locus_tag="VF531_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714267.1"
                     /translation="MNLNYATDMTPNNGSDQLVGIASQFNVEREKDNYYYLALCYFTN
                     LNRKGVSDYFGIKYAPFYSGRMGDGSSKVELSLFPIGVFYNFQAREYVLTFEMLKVGL
                     FLN"
     gene            complement(1496..1939)
                     /locus_tag="VF531_09605"
     CDS             complement(1496..1939)
                     /locus_tag="VF531_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7714268.1"
                     /translation="MESRFILMVEDNSSDVKLAQRAFEKKHVVNELVVVRDGQEALDF
                     LFGTGSYHGRNISDLPAFILLDLKLPKLDGLAVLERVKSDQRTRLIPVIILTTSNENS
                     DLKSAYLLGANSYIRKPVDYNNFVEVVSQLGTYWLGINEAPPVIR"
     gene            complement(1953..>2552)
                     /locus_tag="VF531_09610"
     CDS             complement(1953..>2552)
                     /locus_tag="VF531_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7714269.1"
                     /translation="QILLEDYGAKLDDQGKEYLETVRGEAEHMAELIDALLSLSRVTR
                     SEMHRVKVDLSQLAQKVITELRTAEPQRDVSFTVTPAMTVQADPLLMEAVFQNLIGNA
                     WKFTSKCANPVIEVGMIEQEEMDRPGRTVYFVRDNGAGFDMAYAAKLFTPFQRLHHQE
                     EFPGTGVGLATVKRIINRHGGEIWAEAKEDQGATFYFTL"
BASE COUNT          605 a          732 c          625 g          590 t
ORIGIN      
        1 gagggcttgg gctattatcg ccgggtacgc ttgttacacc agggagcgga agaggtctta
       61 accagatacg gtggaaggct tcccgccgac cccgaggcct tggctgcttt accgggtgtg
      121 ggaccctata tggccggagc actggccagc atcgctttca atctgccggt gccggcggtg
      181 gacggtaatg tgatccgggt agtcactcgg gtattggcct ggaacgaacc ggctaccacc
      241 gcccattctc atcaagtcat ccggacttgg gtggctgagc gctttccgga cggcgccgcc
      301 cgtgaattca cccaggccct gatggagctg ggcgccctga tatgcctacc ccgtaaccca
      361 tattgccggg agtgtccggt acgagatgaa tgccgcggga tgcaaggtaa tccggaacag
      421 tacccggtga aacaggctgc ccggacaatc cctgcccagc gccggatcgt gctgcgcatc
      481 aattggaagg gaagaagatt actggtacaa cggccctcct ccggtctgct cgccggattt
      541 tgggaatatc caaatctaca agtcgaaact ggtgttgacg ctatggtgtt tgcccggcgc
      601 tgggcagaag agcacctggg ccaaacgtta caatttatcc cattcaaaac agtgactcag
      661 atttttaccc accttcgctg ggacttggag gtgttgaccg cggaatggcc tgttgccgga
      721 gaaccgccgc aggtgccacg cggccaatgg tttccttatg aggaggaagt ggaactacca
      781 cgggtgacgt atctcaggca ccttgaggga gacctctccc tcaaacccgc aggcaaaaaa
      841 attatcccgg agtagctttc agcgaataaa atattttgcc ccaaaccaaa aagcggcggg
      901 accgttcctc ggccctaccg cttggtgtac ccaatcacaa tgtttagttt agaaagagcc
      961 cgacttttag catctcaaaa gttaacacat actctcgggc ttgaaaattg tagaacaccc
     1021 cgatggggaa caggcttagc tccactttgg aactaccgtc tcccatgcgc cccgagtaaa
     1081 aaggcgcgta cttgattcca aaataatcgc tgaccccttt gcggttcaaa ttggtaaagt
     1141 aacaaagcgc gaggtaataa taattatcct tctcccgttc cacattgaat tgggaggcga
     1201 tcccaaccag ttgatccgag ccgttgttgg gtgtcatatc cgtggcataa ttcagattca
     1261 ctgcaaatcg cggggtaaaa ccgagactcg cttggaggtt aaaatagctt ccaccgctta
     1321 aaccactggc tttggagata ctttcctggg aaaaaccaaa tcccagctgg atctgatcat
     1381 tgggggcact ccagccatca gcccatacag actagcctcg cgccccgcgg ggcgcgctac
     1441 tcatgcccca cgcctctcgc ctattgcctg ttccctgttg cctgattttc cctttctacc
     1501 ggatcacggg aggcgcctca ttgataccca accaataggt tccaagctgg ctcacgactt
     1561 caacgaagtt gttgtaatcg acgggtttcc ggatataact gttcgccccc agcaaatatg
     1621 cgcttttaag gtcactattc tcattggagg tcgtgaggat gatgaccgga attaaccggg
     1681 tccgttgatc ggacttgacc cgttccaaaa ccgcaaggcc atccaacttc ggcaatttca
     1741 agtccagtag gataaacgcg ggtaggtccg agatgttgcg tccatgatag gaaccggtcc
     1801 cgaagagaaa atccaatgct tcctggccat cgcgcaccac taccagctca tttacgacat
     1861 gttttttttc aaatgcccgc tgagccagct tcacatcact ggaattgtcc tccaccataa
     1921 gaatgaaccg actctccata agaatcgctc ccttataacg tgaaataaaa agtcgctccc
     1981 tggtcttcct tggcctctgc ccaaatctcc ccaccatgcc gattgatgat ccgctttacc
     2041 gtcgccaagc ccaccccggt tccggggaac tcctcctgat gatggaggcg ttgaaaagga
     2101 gtaaaaagct tggcggcata tgccatatcg aaacctgcgc cgttatcgcg gacaaaatac
     2161 accgttcgcc ccggccggtc catctcctct tgttcaatca tcccgacctc tattacagga
     2221 ttcgcacact tactcgtaaa tttccaggca ttaccgatca aattttgaaa taccgcctcc
     2281 atcaacaaag gatccgcctg tacggtcatg gcgggcgtaa cggtaaaaga aacatcacgc
     2341 tgcggttcag cagttcgcag ttccgtgatg accttttgcg ctagttgact aagatcgacc
     2401 ttaacccggt gcatctcact tcgggtcaca cgtgacaatg atagaagcgc atcgatcaac
     2461 tcggccatgt gctcggcctc gccacggact gtctcaagat attccttgcc ctgatcatcc
     2521 agctttgcgc cgtagtcctc caacaatatc tg
//
LOCUS       DASVMB010000339         5876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_483471,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000339 DASVMB010000000
VERSION     DASVMB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483471"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VF531_09615"
     CDS             complement(<1..584)
                     /locus_tag="VF531_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714270.1"
                     /translation="MLAILTGAGGQTALANESTDRIILTSQWRTAETSNTVLKFNSKY
                     NGEWLELDWGFRIPDRLDEDNYASVYSGFELPQIIPDLEFDLDYRWDDTYQIWTAGAA
                     YHMQPSSAWEWGVGYGGGYRDGLDTGGSPYRYRFRKADAKLDFTQNPWSYSAGLSYTA
                     KDYPVTRYYTSERLALEQTIRWQPKRSSALYLSYL"
     gene            complement(775..1146)
                     /locus_tag="VF531_09620"
     CDS             complement(775..1146)
                     /locus_tag="VF531_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714271.1"
                     /translation="MQAEQLLDEAEVLFEKAEEQSETNLNAGLEYFRQGIGNLCRAFL
                     TVNSQEPLGDLRALYFQCRKLEPEFETIDEVMDYFIDPGSGVDDPEIFNDAANEIWDF
                     IVSLLPEEDDDLGDEGDLGDE"
     gene            1318..1926
                     /locus_tag="VF531_09625"
     CDS             1318..1926
                     /locus_tag="VF531_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714272.1"
                     /translation="MILFSRNQVIIVAFVGNSDLLERSGINQEVINKELGCPTQALQQ
                     ATQRERIRANVNVTSKAATTVPPAMDDDTPGGKKTVKINNSPLVKFKSGSEDKSGYEV
                     VSVTVSEPHQPESPEDLPSAPYFKEVALKVMLCIENSQLADNKKDEARRLFDDISFEL
                     QQSPTDWNRVISLLKKGFDYGIKIGAELAKVAEIYYDAKVLA"
     gene            complement(1998..2582)
                     /gene="amrA"
                     /locus_tag="VF531_09630"
     CDS             complement(1998..2582)
                     /gene="amrA"
                     /locus_tag="VF531_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX7714273.1"
                     /translation="MKPTIDRKYLNEGEEQTLLRLARRVLTEFVKNGTDEFPDTVLGE
                     FELTPALRQELGVFVTLTEGEALRGCIGYIVGLMPLYQGVISNTQNAAAHDPRFMPVR
                     PEELAAIQMEISVMPPLQKVKGPEEIQVGRDGVVLKNRFNQGVFLPQVPLEQGWDKTT
                     YLEQLGFKAGLDQDAYQDPETELFRFEAQVFGEE"
     gene            complement(2579..3469)
                     /gene="amrB"
                     /locus_tag="VF531_09635"
     CDS             complement(2579..3469)
                     /gene="amrB"
                     /locus_tag="VF531_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX7714274.1"
                     /translation="METRQKESDRHSLRQPMHAGSFYPGDPQELRRTLDELLGAVCAE
                     ARETPRAVIVPHAGYQFSGPAAACSYKVLRDGAEAIKRVILLAPSHYSYVRGVVLVEA
                     DYQTPLGVYPVDGPAIQTLKETGFPWETNRVAATGEHSDEVQIPFLQTVLPQAKLVPM
                     IVGDLDETERAEAARTIVKVSDEQTVIVTSSDFTHYGKRFGFAPALGGDTRAGIYRLD
                     QGAIERLPEGADSFRDYIKSTGATICGYNPITLLLEIFAAQDWPIRPQLLQYYTSGDL
                     TDDWENSVSYAAVALGALVS"
     gene            3783..4076
                     /locus_tag="VF531_09640"
     CDS             3783..4076
                     /locus_tag="VF531_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7714275.1"
                     /translation="MNVSQLIADTHDLVGILLRRFHRKKVFVVGHSWGTMRGALTIQK
                     FPELFYAYVGVGQVATPKANEELSKELIWFENSAHSLMYEEPGKFHDVLIQNI"
     gene            complement(4116..>5876)
                     /locus_tag="VF531_09645"
     CDS             complement(4116..>5876)
                     /locus_tag="VF531_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7714276.1"
                     /translation="SNIHAENYDEKLNLQNLNHQTMIFSDYRKKESLNGNWHFTIDPY
                     DTGLRSNWQILEAQDASGKKLPWDYDYDGGDDIPVPSCWNLYRPEYFYYEGSAWYSRS
                     FSYCPEMDGERVFLRVGAANYDTKVFLNEEFLGNHYGGSTPFYIELTGKLKERNIIQL
                     CVNNHRTLDRVPMRNTDWFNYGGVYRDVDLYRLPENFIKKFFICLAPDDSYQTIQVDV
                     AVAKEDSEDEVLFEIPELNISKSVKLQNGSGRFTVKAQPELWTPEHPRLYETIVTCRD
                     DQIRDRVGFRQIKVQGTQILLNGKDIFLRGICVHEDDAELGKSSNEADIRRRFADAKE
                     LGCNFMRLAHYPHTELAAKIADEVGLLLWEEIPVYWAIDFENPHTYQDAENQLIELIQ
                     RDRNRASVIIWSVGNENPDSDPRLSFMSRLAQTAKRWDPSRLVSAACLVNHVKIKIED
                     RLTAYLDVIGMNEYYGWYNRDFSELVSLGKNSSPDKPVIISETGADALAGYHSSGTDL
                     FSEEYMEKVYQKQVEIIRKLDYIKGFSPWILYDFRASRRYNKYQKGYNRKGLIAADKK
                     TRKLAFYVLQRFYREKALEN"
BASE COUNT         1455 a         1564 c         1341 g         1516 t
ORIGIN      
        1 aggtatgaca ggtatagggc ggatgaacgt tttggttgcc agcgtatagt ttgctccaag
       61 gcaagccgtt ccgaggtata ataccgggta acggggtaat ctttggcggt ataggagagt
      121 ccggcactat agctccaggg attttgagtg aaatccagtt tggcgtcggc cttccggaac
      181 cggtaccggt aagggctgcc acctgtatcc aagccgtcac ggtaaccccc gccatagccg
      241 accccccact cccaagccga ggaaggctgc atgtggtatg cggcgccggc agtccatatt
      301 tggtaggtat cgtcccagcg gtaatccagg tcaaactcta aatccggaat gatttggggc
      361 aactcaaaac cggaataaac cgaagcgtaa ttatcctcat ccaaccgatc cggtatgcga
      421 aatccccaat ctagttcaag ccattcccca ttatatttgg agttgaattt taagaccgta
      481 ttgcttgttt cagccgttcg ccactgggag gtcaggatga tccggtccgt gctttcgttg
      541 gctaaagcgg tttgcccacc ggctccagtc aatattgcca gcataccaag tagtactccc
      601 gtacattgaa ggcgtgacat cgatcatcac caccattctt tttaggaata ctattcggct
      661 aaagtcataa ttctcctttt attcttacaa ttctgcctta atctgttcct ttaacctgca
      721 attaccgtat tacggacgaa taacgaataa ccaatagcta tgaaccgtat cctcctactc
      781 gtcccctaaa tctccttcat ccccgagatc gtcatcctcc tcgggcaata ggctgacgat
      841 gaaatcccag atctcattgg ccgcatcatt aaagatctcc gggtcatcca ccccggagcc
      901 cggatcgatg aaatagtcca tcacctcgtc aatggtctcg aattccggct ccagtttgcg
      961 gcattggaaa tacaatgccc tcaagtcgcc taaaggttct tggctgttca ccgtcaaaaa
     1021 agcccggcat agattgccga ttccctgccg gaaatattcc agaccggcgt tcaaattggt
     1081 ctcactttgc tcttcagcct tttcgaaaag cacttctgcc tcatccaata gttgttccgc
     1141 ttgcataggc cgttccccct ccgccaccgc cgcgtatacg ggcggttatt gcgcgtgcat
     1201 gcgcgttttt gtcatagtta tttcttggcc gcgtttcaat tccctcttta ttatccgatt
     1261 ttctatgaaa atcatgagtc cacttgccaa attcagggaa gccggattat aataaagatg
     1321 attctatttt cgaggaatca ggtgataatc gtggcatttg tgggaaacag cgatcttttg
     1381 gagcggtcgg gtatcaacca ggaagtgatc aataaagagt tgggctgccc tacccaggcg
     1441 ttgcaacagg caactcagag ggaacgaatc cgggctaacg tcaatgttac ttccaaagca
     1501 gcgactacag tccctccggc aatggatgac gatacgcctg gcggtaaaaa aacggtcaag
     1561 atcaataatt cacccttggt caaatttaaa tccgggtccg aggacaagtc cggttacgaa
     1621 gtggtttcgg tgactgtctc cgaaccacat cagccggaaa gtccggagga tctacccagc
     1681 gcgccctatt ttaaagaggt tgctctaaaa gtcatgctct gtatcgaaaa ctcccagctc
     1741 gccgataata aaaaagacga ggcacggcgg ttattcgacg acatcagctt tgaactgcaa
     1801 caatccccga ctgactggaa ccgggtcatt tctttattga aaaagggttt cgattacgga
     1861 atcaagatcg gagcggaact ggccaaggtg gcggagattt attacgacgc caaggtgtta
     1921 gcgtaaaccg tgaaattcat gattttatgt gttaaacact ggcaggaatc aacttttcac
     1981 actttaccct tgacaattca ctcttcccca aacacttgcg cctcaaagcg gaacagctcg
     2041 gtctccggat cctggtaggc atcctggtcc agccctgcct tgaaccccaa ttgttcaaga
     2101 taggtggttt tatcccagcc ttgttccagg ggtacctggg gtaggaaaac cccctggtta
     2161 aaacggttct taagtaccac tccgtcgcgg cctacctgga tctcctccgg gcctttcacc
     2221 ttttggagcg ggggcatcac cgagatctcc atttggatcg ctgccaactc ctccggccgc
     2281 accggcatga accgcgggtc atgggcagcg gcgttttgag tattggagat cacgccctga
     2341 tacaacggca tcaatcccac gatgtagccg atacagcccc gcaaagcttc cccctcggta
     2401 agggttacga agactcccaa ctcctgccgg agagccgggg tcagctcaaa ctcccccaaa
     2461 acggtatcgg gaaattcgtc agtgccgttt ttcacaaact cggtcagtac ccggcgtgcc
     2521 aggcgcaaca gcgtctgttc ctcgccttca ttaaggtatt ttcgatcgat ggtcggtttc
     2581 atgaaaccag cgcccccaat gccactgcgg cgtaacttac tgagttttcc caatcgtcgg
     2641 tgagatcgcc gctggtatag tattgcaaca attgcggccg gatcggccag tcctgagctg
     2701 caaaaatctc aagcaacagt gtaatcgggt tgtacccgca aatggtcgcc ccggtgcttt
     2761 taatataatc ccggaaggaa tcggctccct cgggaagccg ttcgatagcc ccttgatcca
     2821 gccggtagat cccggcccgg gtgtccccac ccaaagccgg ggcgaagcca aaacgttttc
     2881 cgtaatgagt gaaatcggaa ctggtcacaa tcacagtttg ttcgtcactg acttttacga
     2941 tggtccgggc ggcctcggcc cgctccgttt catcaagatc accgacaatc atcggcacca
     3001 acttggcttg aggtaatacg gtctgcaaga acgggatctg gacttcgtca ctgtgttccc
     3061 cggtcgctgc cacccggttc gtctcccagg gaaatccggt ctctttcaaa gtttggattg
     3121 ccggaccgtc caccgggtaa acccccagcg gagtctggta gtcggcctcg actaaaacca
     3181 cacccctgac ataactgtaa tggctgggtg ccagcaagat aacccgcttg atcgcttccg
     3241 ccccgtcccg taagacttta tagctgcagg cagcggccgg accactgaat tggtagccgg
     3301 catggggcac aatcactgcc cgcggtgttt cccgggcttc cgcgcagacc gcgccgagca
     3361 gttcatcaag ggtcctccgg agttcctgcg gatcgcccgg ataaaaacta ccggcatgca
     3421 tcggctgacg gagcgagtgg cgatccgact ccttctgcct tgtttccatc gtaatccccc
     3481 gtttccaacc aaatcgtttc caaaccttgg attcaccctt ggtatcaact tacaatatat
     3541 ttatgatctc caaaaaatgt attggctact ctacataaaa taccttccgc gacatggact
     3601 ctaataaaaa ggtgtatgat cgacatctct atataacgaa agtaaattga agatcgtttc
     3661 gatgcattaa tccaattata aaattttcat atggaagaag gtttatccct tttttttgga
     3721 agaaattata gaggaatcaa cttgaacctg atcataactc ccataaataa aatcttgaga
     3781 ccatgaacgt cagccaattg atcgctgata ctcatgatct cgttggaatt cttttaagac
     3841 gtttccaccg gaaaaaagtc tttgtcgtcg gacattcgtg gggaacgatg cgaggagccc
     3901 tcaccataca aaaatttccg gagctctttt acgcttatgt cggtgtcggg caagtggcaa
     3961 ctcccaaagc aaatgaggag ctttccaagg agctaatctg gttcgaaaac tccgcccaca
     4021 gtctgatgta tgaagaaccc ggaaaattcc acgatgtttt gattcaaaat atatgaccgg
     4081 aagcggactg agccataaaa acggcccgct cgttactaat tttcgagagc cttttcccgg
     4141 taaaaccttt gtagtacgta aaaggccagt ttacgggttt tcttatccgc ggcgatcagg
     4201 cctttgcggt tatagccctt ttgatatttg ttgtaacggc gtgaagcccg gaaatcatat
     4261 agaatccaag gagaaaaccc tttgatgtag tctagtttcc tgatgatctc gacctgtttt
     4321 tgatagacct tttccatgta ttcctcggaa aacaagtcgg ttccggaact atgataaccg
     4381 gccagcgcat cggctccggt ttcggagatg atcaccggtt tatccggact cgagttcttg
     4441 cccaaactga cgagctccga aaagtcccgg ttataccagc cgtaatattc gttcattccg
     4501 ataacatcca aatacgcggt taaacggtcc tcaattttaa tctttacgtg gttcaccaga
     4561 caagcggcgg agaccaaacg ggacgggtcc caccttttcg cggtctgcgc caaccggctc
     4621 atgaaagata agcggggatc cgaatccggg ttttcattgc ccaccgacca tatgattacg
     4681 ctggcccggt tgcggtctct ttgaatcaat tcaatcaatt ggttctccgc gtcctgataa
     4741 gtgtgtgggt tttcaaaatc gatcgcccag taaaccggga tctcttccca taacagcaat
     4801 ccaacctcgt cggcaatttt ggcagccaat tccgtatggg ggtaatgggc gagcctcata
     4861 aagttacaac cgagctcctt ggcgtctgca aatcttctgc ggatatccgc ttcatttgag
     4921 gacttgccaa gctccgcatc atcttcgtga acacaaattc cacgaaggaa aatgtccttc
     4981 ccattcagca atatttgggt tccttgaacc ttaatttggc gaaatcccac tctatcccta
     5041 atctgatcat cccggcaggt aacaattgtt tcataaagcc ggggatgctc cggcgtccat
     5101 agttccggct gagccttgac tgtaaaacgg ccacttccat tctgaagctt cacggatttt
     5161 gagatattca gttccggtat ttcaaagaga acttcatctt cgctatcctc tttcgccacc
     5221 gccacatcaa cctgaatggt ttgatatgaa tcatccggcg ctagacagat aaagaatttt
     5281 ttgatgaaat tttcgggcag ccgatagaga tcaacatccc gatacactcc gccgtaatta
     5341 aaccaatcgg tgttccgcat ggggacacga tccaaagtcc ggtgattatt cacacaaagc
     5401 tggataatgt tccgttcttt taatttcccg gtcagttcta tataaaacgg ggtcgatccg
     5461 ccgtaatggt tgcccaggaa ttcctcattt aaaaaaactt tggtgtcgta attcgccgct
     5521 cctaccctta gaaataccct ttcgccgtcc atttccgggc aatacgaaaa actcctcgag
     5581 taccaggcgc tgccttcgta ataaaaatac tcgggcctgt acagattcca acaggaggga
     5641 acgggaatgt cgtctccccc gtcataatcg tagtcccagg gtaatttctt tccggaggcg
     5701 tcttgcgcct caagaatctg ccagttactc cgcaaaccgg tatcataagg gtcgattgta
     5761 aaatgccagt tcccgttgag actttccttc ttccgataat ccgagaagat catggtttga
     5821 tggtttaaat tttgcagatt aagcttttca tcgtagtttt cagcgtgaat attcga
//
LOCUS       DASVMB010000340         6922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_557877,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000340 DASVMB010000000
VERSION     DASVMB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557877"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VF531_09650"
     CDS             complement(<1..1157)
                     /locus_tag="VF531_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7714277.1"
                     /translation="MSLLQVNNLDKFYGATPIIREGTLAIQAGEKWGLVGRNGCGKST
                     LIKVLTGKEDYDQGEIGWAKGTRIGYLEQEPEFGPGNSIYQELRGIFGDLDLLQETIT
                     HLQEELNQPGLANETLNRLVEEFHSKTEEFEAAGGYQLEGRIQGVLRGLGFSKERWND
                     PTTVLSGGERTRLALAKLLLSPNDILFLDEPTNYLDLAAIEWLEEFLGTYRGAVLLVS
                     HDRYFLDRVVSGIIELEFCRIKRYRGNYSAYRSQKEAEYQVALKAYNEQQKELERQEK
                     FVRESRATEKSKRKAHSIEKRLAQVERIERPVWDDQAFKLSFGEPQPSSRKVLELEGI
                     AKRFGNKVLLDGVDLQIEAGEKIGLIGPNGAGKTTLLKMIMGLETPDRGGIR"
     gene            complement(1255..1836)
                     /gene="lepB"
                     /locus_tag="VF531_09655"
     CDS             complement(1255..1836)
                     /gene="lepB"
                     /locus_tag="VF531_09655"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7714278.1"
                     /translation="MAKDHVQRSGGSGSDKPKAKEYSSLKFLRDIIEVVVPAIILFLI
                     INQFIEHTVVPSGSMIPTIQIEDHFFLNKTAYWFHKPQRGDIVVFKPPASMGKTDFVK
                     RLIGLPGEKIAIRHGIVYINDHPLKEPYITPDRAPIEDFGPYLIPDGRYFMMGDNRNN
                     SRDSRYWGTVPLKDVRGKAWWRFWPFNQMGIIK"
     gene            complement(2137..2889)
                     /locus_tag="VF531_09660"
     CDS             complement(2137..2889)
                     /locus_tag="VF531_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7714279.1"
                     /translation="MNVVYAKKWLIGALVLLFVFCFGYLCGDLFHDAFPVSTPLTREM
                     PVFFVKTPKHQACLTFDISWGEKTLPLVLKILADNQVPATFFVSGPWATGHPDAVRAI
                     VGGGHEVASHGDRHVNLSQYSQEEVRTNISKAHDALLTLTGRVAPFFRPPNGDYDDLV
                     IDTARELGYETVIWSVDSLDWKNPGPDYMVNRILDRVFPGAIILCHASDSSKQIDQAL
                     PGIIRGLREKGYKLVSLSQLFGAGVPGRSDPR"
     gene            complement(3038..3316)
                     /locus_tag="VF531_09665"
     CDS             complement(3038..3316)
                     /locus_tag="VF531_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7714280.1"
                     /translation="MTKTELIDKVAEKSGLTKKDSGKAIDAVINAMTQALSKGDKVQL
                     VGFGSFEVRKREARKGRNPQTGAEIKIAARKVPVFKAGKALKDAVGKK"
     gene            complement(3703..4234)
                     /locus_tag="VF531_09670"
                     /pseudo
     CDS             complement(3703..4234)
                     /locus_tag="VF531_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(4602..5129)
                     /locus_tag="VF531_09675"
     CDS             complement(4602..5129)
                     /locus_tag="VF531_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714281.1"
                     /translation="MEIFKLTENNIPNVAKLMAYIKPDWWDYEGGVMQLSDIRTTVDN
                     VGWFMGEDEQHPKGWILCAEYSCYSCLSIECLGYDENGKFVMEHQLQPLLEKAEQYAR
                     DKGFRILKYMIGSVDMSCHGRELGNYWEALRDLESYGREHFDYFVRYGFKPAGFMPNC
                     LGENYHCIIMIKELK"
     gene            complement(5235..6137)
                     /locus_tag="VF531_09680"
     CDS             complement(5235..6137)
                     /locus_tag="VF531_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HEX7714282.1"
                     /translation="MKKYTVYQVDSFTKDLFKGNPAGVVLNADGLSEAQMQMIARELN
                     NSETSFLFSPDSSECDGVIRYFTPKTEVPICGHATIAAMYVKALAENLSSSVFRMRTK
                     IGVLPFEIIKIHNDYTIVMTQGEIRFETPFALEIKEKILKGLGLTENELDDRCPVQTV
                     STGHSKVMIGIKNRERMNQLAPNFTALSELSRIINCNGYFVFTFDSDSKDILTYGRMF
                     APAVGINEDPVTGNAHGPLGAYLVHHRLVDASDGEFTFKGRQGEAINRLGTVEVRVKI
                     ENNQPVLVQIAGEAVIVFKTEIEI"
     gene            complement(6423..6800)
                     /locus_tag="VF531_09685"
     CDS             complement(6423..6800)
                     /locus_tag="VF531_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7714283.1"
                     /translation="MKLSDICIITEDVPLLTRFYENVLQVKAEGNEIHAFIAIGGEGI
                     AIYSKKAAENDMRFDFSKHWGSGNFTIGLNVEDVDKEYERLKSLDLEFVTTPATYPWG
                     ARSFHFRDPDGNIICFRTIVNKS"
BASE COUNT         1872 a         1725 c         1362 g         1963 t
ORIGIN      
        1 cggatcccgc cgcgatcggg agtctcaaga cccatgatca tcttgagtaa agttgtcttg
       61 ccggcgccgt tgggtccgat taaaccgatc ttctcacccg cttcgatctg gagatccacc
      121 ccgtccagca ggactttatt gccaaaacgc ttggcgattc cctctagttc caataccttt
      181 ctcgaactgg gttggggttc tccgaaagag agcttgaagg cttgatcatc ccaaacggga
      241 cgttcgatcc gttccacctg ggccaaacgt ttttcaatgc tatgcgcctt gcgtttactc
      301 ttttccgtgg cacgggattc acggacaaac ttctcctggc gctccaattc cttctgttgc
      361 tcgttataag cctttaacgc cacttgatac tcggcttcct tctgggaacg ataggccgaa
      421 taattacccc ggtaacgctt gatgcggcaa aactcaagtt caatgattcc cgaaaccacc
      481 cgatccagaa aataacgatc gtgggatacc aaaaggactg cgccgcgata ggttccgaga
      541 aactcctcaa gccattcgat ggccgccagg tccagataat tggtgggctc gtccagaaac
      601 agaatgtcat tgggagatag cagtagcttg gccagagcca gacgggtccg ctcccctccg
      661 cttaacaccg tggtcggatc gttccaacgt tccttggaaa agcccaggcc gcggaggacg
      721 ccctgaatcc ttccttccag ctgataaccg ccggctgctt caaactcctc ggttttggaa
      781 tgaaactcct ccaccagacg gttgagggtt tcgttggcca ggccaggttg attcaactct
      841 tcctgcagat gagtgatggt ctcctggagg agatccaagt cgccaaagat tccccgcaat
      901 tcctgataaa tggagttacc tgggccaaat tccggttcct gttcaaggta gccgatacgg
      961 gtccccttgg cccagccgat ttcgccctga tcgtaatctt ccttgccggt caataccttg
     1021 atcaaggtgg atttgccgca accgttccgg cctaccaaac cccatttttc accggcctga
     1081 attgccaagg taccctcccg gatgatggga gttgcgccat aaaacttatc gagattatta
     1141 acctgaagta aggacatctg ctttccctta tttacacgac gaaaaggcca gggctctcca
     1201 tatggataaa actttgagaa cagcggcctt tcatcttgat ttttttgata ctgattattt
     1261 tataataccc atctggttaa aaggccagaa tcgccaccag gccttgccgc gaacgtcttt
     1321 gaggggtact gttccccaat agcggctgtc gcggctatta ttccggttgt cgcccatcat
     1381 gaagtaccgg ccatcgggga tcaggtaagg tccaaaatcc tcaatcgggg cccgatccgg
     1441 agtgatatac ggttccttca agggatgatc attgatataa actataccgt ggcgaattgc
     1501 aatcttctca ccgggcaacc cgatcagccg tttcacaaaa tcggtcttgc ccatgctcgc
     1561 cgggggtttg aaaaccacaa tatccccccg ttgaggctta tggaaccagt aggcggtctt
     1621 attcaaaaag aaatggtctt cgatctgaat cgtaggaatc attgagcccg agggtactac
     1681 ggtatgctcg atgaattgat tgataatcaa gaacagaatg attgccggga caacaacttc
     1741 aatgatgtcg cggagaaact tcagcgacga atactctttg gccttgggtt tatccgatcc
     1801 ggaccctccg gaccgctgga catgatcttt cgccattggt ttcacttcct aaatttaaaa
     1861 ggtttatctt tagttaacgt aatcaacccg aaaaaatttc aataggtcaa ttataacata
     1921 aattaatctg tacgcaatgt ttcaagttgt cggctccgct aaactccgtc ttgatctgtt
     1981 taaacctgat ccattcggaa aaggtgttat ccacaatgca taaaaaatcc cgacgcaaac
     2041 atcgggataa acatagttca aagtcataag tccaaagccg aaaatccact tggaaacata
     2101 tattcaatgg ttttctttac acttcactct ttttatttag cggggatcac tccggccggg
     2161 cacccccgcc ccgaataact gagaaaggct tactaattta tatccctttt cacgcaaacc
     2221 ccggatgata ccgggaagcg cctgatcgat ctgtttactg gagtcgctgg catgacagag
     2281 aatgatcgcg cccggaaata ctctgtctag aatccggtta accatgtaat ccgggccggg
     2341 gtttttccaa tccagggagt ctaccgacca gatgactgtt tcgtaaccca gttcccgggc
     2401 ggtatcgatc accaaatcat cataatcgcc attgggtggt ctaaaaaaag gtgccacacg
     2461 tccggttaag gtcaacaggg catcgtgagc tttactgata ttggttcgga cctcttcctg
     2521 ggaatactgg ctgagattga cgtggcggtc tccatgactt gcaacctcat gaccgccgcc
     2581 gacaattgcc cgaaccgcgt cgggatgtcc cgtcgcccac ggcccggaaa caaaaaaggt
     2641 tgccggaact tgattgtcgg ctaaaatttt taaaaccagc ggtaaagttt tctcgcccca
     2701 actgatatca aaggttaaac aggcctgatg tttgggggtc ttaacaaaaa aaaccggcat
     2761 ctcccttgtc agtggggtac tgacgggaaa tgcatcatga aaaagatcgc cgcacaaata
     2821 accgaaacag aatacgaaga gcaagaccaa ggcaccaatc aaccattttt ttgcgtaaac
     2881 aacattcatc caggtcaccc cccgccataa tctattcaac accatgagag gtattacacc
     2941 atactgttcc tttcaccaaa gaagaataaa aaggaatgga cccatatgga tccattcctt
     3001 cttcaatcat tgattaagta atcatggatt gaaaaaatca cttcttgccg actgcatctt
     3061 tcaaagcttt accggcttta aaaacaggaa cctttctagc tgcgatttta atctcagcac
     3121 cggtttgggg attacggccc ttacgggctt cccgtttgcg gacttcgaag ctaccgaagc
     3181 ctaccaactg gactttgtcc cctttggaca acgcttgggt catcgcattg ataaccgcat
     3241 caatagcctt cccagaatct tttttggtca aaccactctt ctcggccact ttatcgatga
     3301 gttcggtttt ggtcatcaac agttcacctc cttccgaaaa aagtggaaaa taatttgttg
     3361 attcgcaagg aatgtcctgg taaattactc ttgttttatt ctagattctt cccctatttc
     3421 ctgcttttca aacgaaaaaa accaactttt tttctacatg gcactgtgtt tcgtggatta
     3481 ttccagctag aatgattttt taaccttcat tctcatagac gtatcgtcct tctctactaa
     3541 cattcatgac ttcacccggc tatcgaaaac ttgaattgtt ttatggaaat cctgaggaag
     3601 gaggcaatag aggcaagggg gataactctt catcaagttt attccgttta tctacttcgt
     3661 tcaaaacagc taccctccat aaaaccggaa tcagccaagt gctcaatgtg ctgcttcttc
     3721 tactcgatcc agccattcat tggttaaaca gttaaactgc ttgacttgtt ccaactgtag
     3781 attatggccg gctctatcca aaacggcaaa agacgccctg ggataatgat ttaaaatatt
     3841 ccaggcgtcc tggtatccta ctgaagcatc ttgcctcccc aataaaaaca ggacgggttt
     3901 cgtaaactcc tcttgaaccg catctacatc aaatgtgaac gcgcgactca ccggcaagca
     3961 aaaagttttc ctcgggtatg actttggcaa taaaatcaag taccacctga agcatctgat
     4021 cagaactttg gatccagggt tctcctttgg ttttacccat gcccggcaag tccaaataga
     4081 ttcgccggta gcccggccgt ttttgaaaca caggctccat acaaccgctc atcaagcggt
     4141 gatccgggta atatccgtga agcatcagga ccgggttgcc tgtaccatat tcctcataaa
     4201 aaactgtaat ccgatccagt ttacattccg tgatttcatt cctccttata ttaactcacg
     4261 tttaatgtca aacaccctat ttcaataccg tatgaaagat gcctgaccca tttaccaaga
     4321 gaattcatca atcctcgtgg tatgcattga gatgtaagct ttccgtattt tctgtaaaac
     4381 aggttaaata atagataggg gaccctttgg caacgacttt cgggtattcg cttaacaatg
     4441 tttttgaatg agggagacat tatccttttg aacttagaat ccataggcct taggctcatt
     4501 ttgggaaata tcagtttaat gttcatcttg attttctcca tcgcccgatt gggcatcacg
     4561 gtaggataag aaaattgcta catcacccta tatttgaatg gtcatttaag ttctttaatc
     4621 atgatgatac agtgataatt ttcacccaaa caattcggca taaacccggc cggcttaaat
     4681 ccatatctga caaaataatc aaagtgttcc cgaccatatg attctaaatc tcttagtgct
     4741 tcccaatagt ttccaagttc tcttccatga caagacatat caactgaacc aatcatgtat
     4801 ttcaaaatgc ggaatccctt atcccgggca tattgttccg ctttttccag caagggctga
     4861 agctgatgct ccatgacaaa ctttccgttc tcgtcataac caaggcactc tatacttaag
     4921 cacgaataac agctatattc ggcgcaaagg atccatcctt tgggatgctg ctcatcctca
     4981 cccataaacc aacccacatt atccaccgtg gtgcgaatat cgcttaactg catgactccg
     5041 ccctcataat cccaccaatc gggtttgata tatgccatta attttgcaac gtttgggata
     5101 ttattttctg tcaacttaaa aatttccaca actgctcccc ccaagaataa aaaaatatca
     5161 agtagaacgt tccctcgtgt actcacatag ccttgttgcc atttttacta tttgatcatc
     5221 catctcaaat gaggctaaat ttctatttcc gtcttaaata cgatcactgc ttccccggca
     5281 atttgcacca ataccggttg gttgttctcg attttaactc ttacttcaac tgtccctaaa
     5341 cggttgatgg cttccccttg tctaccctta aaagtaaact cgccatcgga ggcatcgacc
     5401 aatcgatgat ggaccaaata cgctcctaac ggtccatggg cattaccggt aacgggatcc
     5461 tcattgattc cgacagccgg tgcaaacatc cggccataag tcagaatatc cttggagtcc
     5521 gaatcaaagg taaagacaaa atagccgttg caattgataa tccggcttag ctccgatagt
     5581 gcagtaaagt tcggagccaa ttgattcatt ctctcacggt tcttgatgcc gatcattact
     5641 ttagaatggc cggttgatac agtttgaacc ggacacctgt catccaactc gttttcagtt
     5701 aaacccagac ctttaagtat cttttctttt atttcaagag caaagggcgt ttcaaatcgt
     5761 atttcacctt gagtcatgac aatcgtataa tcattgtgga tcttgattat ttcgaatggc
     5821 agcactccta tctttgtccg cattctgaac acggaagaac tcaaattttc cgccagagct
     5881 ttaacataca ttgcggctat cgtggcatgg ccgcatattg ggacttcggt tttcggagta
     5941 aagtaacgta ttacaccgtc acactcacta ctatcgggag agaataaaaa cgaagtttcc
     6001 gagttattca actccctggc tatcatttgc atttgcgctt cgcttaagcc atctgcattt
     6061 aaaaccaccc cggcaggatt tcccttaaat aaatccttgg taaatgaatc tacttgatag
     6121 acagtatatt ttttcataaa ttttcaaccc catatcgggc ttggtatcgc ttcatcctca
     6181 agggaagatg aatctttggt aagaaaggca ttacttcctg ttttcctcaa ttctaaaccg
     6241 aactatcttt tttacttcta caagaagctc ttgatcaaaa agaaattgaa tcgtattttt
     6301 ttcattggtt ttatagtcat acaattcttc tttgaaaaac acaatcgcgg caagactaca
     6361 tagaagccaa tatattttaa aaaagaagga ccctaccggt ccaatcaatt cattctaatt
     6421 atttacgatt tatttacgat cgtgcgaaaa caaatgatgt tgccatccgg atctctgaaa
     6481 tgaaaggacc ttgcccccca gggataggtc gccggggtag tcacaaactc aagatcaagc
     6541 gatttcaacc gttcatattc cttatctaca tcttcaacat ttaaaccgat tgtgaagtta
     6601 cctgaacccc aatgttttga gaagtcaaat cgcatatcgt tttcggcggc tttttttgaa
     6661 tagatggcaa tgccttcccc tcctatagct atgaaggcat gaatttcatt tccttctgcc
     6721 ttaacttgca gtacgttttc gtaaaaccgg gtcagcaacg gtacatcttc cgtaattatg
     6781 catatatccg agagtttcaa tgacgttctc tccttttgac taatcgatgt gacaggctta
     6841 acctaatcct taatagcttt ggtagcgata tatgtaggag tatattttga aatttcatca
     6901 ttaggctcat catcttcata ga
//
LOCUS       DASVMB010000341        10956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_265221,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000341 DASVMB010000000
VERSION     DASVMB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265221"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1557
                     /locus_tag="VF531_09690"
     CDS             64..1557
                     /locus_tag="VF531_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX7714284.1"
                     /translation="MDAKCECEIPASEVRVGDILLVKPGETIPVDGRIISGQTSVDQA
                     IITGESLPVDKAVNDEVYVGTFNQMGTITIIAAKVGEDTSLAKLIRLVKEAEDKKAPV
                     VRVADRWATIIVPAALACAIAVYWLTRDITRAVTILVVFCPCALVLATPAAIMAGIGN
                     ASRKGILIKSGEALEKMGSIDLIAFDKTGTITCGKPEVTEVISLNRDYPEESVLQLAA
                     SAENFSEHPLGQAIVMKAKEESLSITEPHDFQLILGRGIIATVDSHRIMVGNLDLLSN
                     NGVAVPDSLLQQSSFFQRDGKTVMLVVVNGNPAGMIMVADRIKGSVPEAIQELKSKYD
                     LEIMLLTGDNRNTAREIGRQAEIDRIYAGQLPEEKVVVIKEKMNQGKRVCMIGDGVND
                     APALATAAVGIAMGVLGSDIAIEAADIALMSDDIKKIPELIGLAQKTLRTITQNVWIS
                     MSINFGAIVLAAMGLMGPVVGALVHNLGSVLVVVHSAALLRYKGHEA"
     gene            1764..1901
                     /gene="scfA"
                     /locus_tag="VF531_09695"
     CDS             1764..1901
                     /gene="scfA"
                     /locus_tag="VF531_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HEX7714285.1"
                     /translation="MKLVKALVQGKLASTTATGGCGACKNSCQSACKTSCTVGNQVCQ
                     K"
     gene            2012..3358
                     /gene="scfB"
                     /locus_tag="VF531_09700"
     CDS             2012..3358
                     /gene="scfB"
                     /locus_tag="VF531_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HEX7714286.1"
                     /translation="MNVQSANWHVFKALDRFFLFDVLSNSLFQLEEPVFNYLSGKPVS
                     PELRPQIEADLDELRSGGYLATESLAEPALTREPVLKALCLNVSHDCNLRCRYCFAGT
                     GSFGGVREEMSAAVGKAAIDLLLHESGDRRQLEVDFFGGEPLLNFEVVKELVAYGREA
                     SFRVNKAIHFTLTTNGVRLDPTVRDFLNREGISLVLSLDGRPEVNDGMRGKGSYERVL
                     PNFLALVSERNHQNYYLRGTFTARNLDFTEDVRHFYDLGFRELSLEPVVDRDGDFRLT
                     EADLPRITAEYDRLAAYYLEKRSQGQGFRFFHFEIALEHGPCLPKRLTGCGAGFDYLA
                     VTPSGELYPCHQFVGRGNYRMGDVFSGVERIDLRKNFGEATLYKKEGCAGCWSRFYCS
                     GGCHANAELLNGSIYQPDKLGCAMQQKRLELALALKGIAICDNGMKEAEPEKSEFR"
     gene            3421..3828
                     /locus_tag="VF531_09705"
     CDS             3421..3828
                     /locus_tag="VF531_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7714287.1"
                     /translation="MYQDFHLSHQDAAKIVSAIQAELEKNGKGAAVAVVDEHGELLAF
                     LRTDGCRLSSITIAMNKAYTAARECKESKAVGEGSRQKGFPMTNFGELRYVTWGGGVP
                     IAYRGKVIGAVGVSGLAEDEDIQLAQMGAGLVS"
     gene            complement(3929..4906)
                     /locus_tag="VF531_09710"
     CDS             complement(3929..4906)
                     /locus_tag="VF531_09710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7714288.1"
                     /translation="MPKISICMAAYNHQQFVGEAIQSVLDQTFQDWELIITDDASPDH
                     TADVISHYNDPRIKFFKHEKNQGIAITTADCIKKAQGTYIAILNSDDRYRPAKLEKQV
                     QILDERPEVWAVFTHVWFINRYGGKYNKLNIGFYTNKRNIFNKSNRSRREWLNYFFYY
                     NNTLCDPSVMARREVYTEIAPPDPHFPIAGDFNRWVRLCLHHDIFIIPEILLDFRIHQ
                     NASGSSWLTTPPKIDWEYFLILRNYLEIKTPDELQRIFPELYEGQELDPDLIPYYLAL
                     PASKMASRTYRLFAVSVLSDLLDNPDKAAKLDKLCGFTHKDLHKLYKTI"
     gene            5316..6686
                     /gene="rlmD"
                     /locus_tag="VF531_09715"
     CDS             5316..6686
                     /gene="rlmD"
                     /locus_tag="VF531_09715"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX7714289.1"
                     /translation="METPVQPGQKVQLEITSYGHEGEGVGRYQNFTLFIPGALKGEAV
                     EAKVTEIKKNFARGRLLRIIEEIPERRIPACPVYHECGGCQLQHLSYPAQLALKRQQV
                     MDAVERIGGLTGVTVHPTLGMKEPRCYRNKVQYPLAMENGKVSAGFYRKGTHQIVPVT
                     GCLLHSELSNRIVAAVVQKIEENHVPIYNERTGSGLLRHVLIKNAFATGEMMVVLVTN
                     GEKFPSGSKIATELMTEFPAIRSVVQNINRSRGNVILGNQSVVLGGSDAIQDILGGLK
                     FKISARSFFQVNPVQTEVLYEKAVDYAGLAGTETVLDAYCGVGSLTLFLARRARAVYG
                     IEVVPEAIRDARENAALNEMKNVQFLVGETERVLPELARQGIHFDVATVDPPRAGCEP
                     ELLRSLAVNGVGRIVYVSCNPSTLARDLKLLGELGYKTMEIQPVDMFPQTYHVETVVL
                     MSRKDK"
     gene            7003..7638
                     /locus_tag="VF531_09720"
     CDS             7003..7638
                     /locus_tag="VF531_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7714290.1"
                     /translation="MPKTDTKSRILTECLALFSKHGYDGVSMRDIAKAIGFRESALYK
                     HYKSKQEILDSIFSVMKAHFEEASKAISLSDGDDFSVLAHGYKNEGLDVLKKQCSVLF
                     LYWLKDEQAAQYRRLLMLEQFRHSEAENLLREFLMDGALQYQAALFKKMIEIGYFKKI
                     DSEVLALQFYSPIFMLLCKYDSLPDKESEALSILERHVASFDAAYRNEDVK"
     gene            7735..8391
                     /locus_tag="VF531_09725"
     CDS             7735..8391
                     /locus_tag="VF531_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7714291.1"
                     /translation="MICHVNNTNLYYEKSGTGKPLLLIHGNGETHKIFDKAMPLLAEC
                     FTVYAIDSRGHGQSEAVLVYHYDDFAEDIKCFIEELKLEQPILYGFSDGGIVGLLLAA
                     KYPQLLSQIIISGANLDPKGIRTGWLKLFQCINAIVKDPKMTMMLKEPNFTAEMLSHI
                     AIPTTVLAGSRDMVKRSHTQYIADNIPNSRLHILRGEGHGSYVLHSTKIVKMILDATN
                     "
     gene            8630..8821
                     /locus_tag="VF531_09730"
                     /pseudo
     CDS             8630..8821
                     /locus_tag="VF531_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9235..10236
                     /locus_tag="VF531_09735"
     CDS             9235..10236
                     /locus_tag="VF531_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX7714292.1"
                     /translation="MKLYIIQTGYTKVPYGQFFGGLEGWSGLKGKIKFATDKKHFILV
                     PVNCFLIEHPSEGPLLVDTGINREQARKHKEYYTALMGLVTDDDEYVMQPEEEINSQL
                     HAIGYKCSDVKKILLTHLHEDHIGGLKHFPHAKVFVSSQEWLLKDKKILGLIPGVYPK
                     SIAPITDWEVISYSSGAFYNFAKSQDIFGDGSIILLPTPGHTSGHTSVFLQFGDYQIL
                     LCGDCLYTLRHLAVDQVRSVIINSEMTENYIRSIRLTQELQEALPNLVIIPGHDPTEY
                     HEKYLKQFFKDGILSAEELAVVNKYEKQLFESGFHLAKKSMPQFMPVPNNGYVGTVS"
     gene            10506..>10956
                     /locus_tag="VF531_09740"
     CDS             10506..>10956
                     /locus_tag="VF531_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7714293.1"
                     /translation="MVLIETIVDSKAKILTAAKELFARKGFEGTSTREIVNAAGVNIS
                     LIAYHFGGKENVLFALFDHFMDGAYHEDVSFGDSELVEEFKSILIRIIKLRFEEPELI
                     SILHHEIILNSGRTDKIKASLIPVWTRVELLLDEGKRRGLFSFENTKN"
BASE COUNT         3061 a         2127 c         2847 g         2921 t
ORIGIN      
        1 cgttcgtaaa gcgagacaag gcgttgaaaa actggtccgg cttactccga cgatggccag
       61 aatatggacg caaaatgtga atgtgaaatc ccggcctccg aggtaagagt cggagacatt
      121 ttattggtca agccgggaga aaccattccg gtggacgggc ggattatcag cggccagacc
      181 agtgtagacc aagcgattat tacgggggag tccctcccgg tggataaggc tgtcaacgat
      241 gaagtttatg taggtacctt taaccaaatg ggaacgatca cgattatcgc cgcaaaagtg
      301 ggtgaagata cctccctggc taaattgatt cgattggtga aagaggccga agataaaaag
      361 gctccggtag tccgtgtggc cgaccgttgg gcgaccatta ttgtaccggc tgccttggct
      421 tgtgccattg cagtttattg gctaacacgc gatataacca gggcagtaac gattttggtc
      481 gtcttttgcc catgcgcttt ggtgttggcc actccggcgg caatcatggc cggaatcggg
      541 aatgcgtccc ggaaggggat attgatcaaa agcggtgaag cattggaaaa gatgggaagt
      601 attgatctaa tcgcttttga taagaccggg acgatcactt gtggtaaacc ggaagtgact
      661 gaggtcatca gtttaaaccg ggactacccg gaagagagtg ttttgcagtt ggctgccagc
      721 gcggagaact tttccgagca tccattgggg caggccattg taatgaaagc caaggaagaa
      781 tccctgtcca tcaccgaacc ccatgatttt caactgattt taggccgggg gattatcgct
      841 acagtcgatt ctcaccggat catggtcggt aatcttgatt tgttaagcaa taatggggtg
      901 gcggtccccg atagcctgtt gcagcagtct tcattcttcc aaagggatgg aaagacggtg
      961 atgctggtgg tggttaacgg gaaccccgcc ggaatgatta tggtggctga ccggataaaa
     1021 ggttcagtgc cggaagcaat tcaagagctg aaatccaaat atgatcttga gattatgctg
     1081 cttaccgggg ataatcggaa taccgctcga gagataggaa gacaggcgga gattgatcgg
     1141 atttatgccg ggcaactgcc tgaggagaaa gtggtggtta tcaaagaaaa gatgaaccag
     1201 ggaaaaaggg tatgcatgat tggtgacggg gttaatgatg cccccgcctt ggcaaccgca
     1261 gcagtcggga tcgcaatggg cgttcttggg tcggatatag ccattgaggc ggctgatatt
     1321 gccctgatgt ccgacgatat caagaaaata ccggaattga ttgggttggc acagaaaact
     1381 ttgcggacga taacccaaaa tgtttggatc tccatgagca ttaatttcgg tgcgattgtt
     1441 ctggcggcga tggggttgat ggggccggtt gtcggagcgt tggtgcacaa tttaggctcg
     1501 gtattggtag tggtacattc ggcagcgctt ttgagatata aggggcacga ggcgtgaggc
     1561 gtgaagtagc gcgcgcccta tgaggcgcgc agcaataggt gcgaggtaaa ggtgaaaagg
     1621 cgtgttgatc tcgactgtac gggtatattg aacctctgca ggaatgcctt tattgactat
     1681 acttttcggt ttgtgtatac tattaataga tttgattgtg aatcactaaa cgaatggatc
     1741 taaaattgtg gaggtatcgg aagatgaaat tggtaaaggc gttggtacag ggtaaattag
     1801 caagcacgac ggctaccggc ggttgcgggg cttgcaagaa ttcttgccag tccgcatgca
     1861 agacctcttg cactgttgga aatcaggtct gccagaaata accggatttt taccatagcc
     1921 tgtccatcaa gccatgcgac acaccatggc ttgatttatt tctatcagct attaaaatga
     1981 cgaatactat actcggatcg gagcgatggc aatgaacgtg caatctgcca actggcatgt
     2041 ttttaaagcg ctggatcgct tttttttgtt tgatgttctt tccaactcct tatttcaact
     2101 ggaggaaccg gtcttcaact acttaagcgg taaaccggtt tccccggagt tacgtcccca
     2161 gatcgaggcc gatctggatg aattgaggag cgggggctat ttggcaaccg agtcgcttgc
     2221 ggagccggcg ctcacccggg agccggtatt aaaggcatta tgccttaacg tttcccatga
     2281 ttgcaatctt cgttgccggt attgttttgc cgggaccgga tctttcgggg gcgtccgtga
     2341 agagatgagc gctgctgtgg gaaaggcggc aattgatcta ttactacatg aatccgggga
     2401 tcgccgtcaa ttggaagtgg atttcttcgg aggcgaaccg ctgctcaact tcgaggtggt
     2461 aaaggaactg gtggcttacg gccgtgaggc ctcgttccgg gtgaacaagg cgatccattt
     2521 caccctaact accaacgggg tgagactgga cccgaccgtt cgtgattttc tgaatcggga
     2581 aggaatctct ctggttttat cgctggacgg acggccggaa gtcaacgacg ggatgcgtgg
     2641 caagggcagt tacgagcggg tgttgcccaa ctttttggcg ctcgtttctg aacggaacca
     2701 ccaaaattac tatttgcggg ggaccttcac cgcacgcaac ctcgacttca ccgaagatgt
     2761 gcggcatttc tacgatctcg gttttcggga gctttccctg gagccggtgg tcgatcggga
     2821 cggggatttc cggctcaccg aggccgactt gccgcgaatt acagccgaat acgacagact
     2881 ggccgcttat tatttagaaa aacgcagcca ggggcagggt ttccgcttct tccactttga
     2941 gatcgcactg gagcacggac cttgccttcc caaacggctc acaggttgcg gtgcgggatt
     3001 cgattacctg gcggtaacgc cgtccggcga gctatatccc tgccatcaat tcgtgggacg
     3061 cggcaattac cggatggggg atgtcttttc cggggtggag cggatcgatc tccgtaaaaa
     3121 ttttggagag gctactttat acaagaaaga aggctgcgcc gggtgctggt cacggtttta
     3181 ttgcagcggc ggctgccatg ccaatgccga attactgaac ggatcgattt atcagccgga
     3241 taaattaggc tgcgccatgc agcaaaaaag gttggaattg gctttggcgc ttaagggaat
     3301 cgccatctgc gacaacggca tgaaggaggc ggagccggag aagtcggagt tccggtaagt
     3361 agcgtcacgc tatagcgtgg cgttacgtga cgctaccaaa ttgttttggg agggattgga
     3421 atgtaccaag attttcattt gtcccatcaa gatgccgcaa aaatcgtctc tgccattcag
     3481 gctgaattgg agaagaacgg taaaggggcg gcagtcgccg tggtggacga gcatggcgag
     3541 ttgttggctt ttttacgcac cgatggttgc cggttgtctt ccattaccat cgccatgaac
     3601 aaggcttata ccgctgcccg ggaatgtaaa gaatccaagg ctgtgggaga aggttcccgt
     3661 cagaaagggt ttcccatgac gaactttggt gaactgcgtt atgttacctg gggtggagga
     3721 gtccccatcg cgtatcgggg gaaggtcatc ggggccgtgg gcgtcagtgg tctggcggaa
     3781 gatgaagata ttcagctggc ccaaatgggg gcagggcttg tttcttaaac attatataaa
     3841 tatagggctg cctcaaagtg gttgagacag cccgtctcca tttgtctcag ggcaaggcct
     3901 aggatgtatt aaataaacta ccagtcaatt atatagtttt atagagttta tgaagatctt
     3961 tatgagtgaa tccacatagc ttatcaagct tcgcagcttt atccgggtta tcaaggagat
     4021 cggataggac agatacggca aacaaccggt aggttcgtga ggccatcttc gatgccggga
     4081 gcgccaggta ataagggatg agatccggat ccaactcttg cccctcgtat agctcgggaa
     4141 agatccgctg tagttcatcg ggggtcttga tttccaaata gttccggagg atcaggaaat
     4201 actcccaatc aatcttaggt ggggttgtta accaggagct gccgctggca ttttggtgaa
     4261 tcctgaagtc gagcagtatc tcggggatga taaagatatc atggtgtaga caaagtcgca
     4321 cccagcggtt gaaatctccc gctataggga aatgagggtc ggggggtgca atttctgtat
     4381 atacttcgcg gcgggccata acgctggggt cgcaaagagt gttattatag taaaagaaat
     4441 aattcagcca ttcccggcgg gagcggttgg acttattaaa aatgttgcgt ttattcgtat
     4501 aaaaaccgat gttgagcttg ttatattttc cgccgtaacg attgatgaac cagacatggg
     4561 taaaaaccgc ccagacttca gggcgctcat ccagaatctg aacctgtttt tcgagtttgg
     4621 cggggcggta tcgatcatcg gaattcagga tggcgatata ggtaccctga gcttttttaa
     4681 tgcaatcagc agtggtgatt gcaatccctt gatttttttc gtgtttgaaa aatttgatcc
     4741 ggggatcgtt atagtggctg atcacatcgg ctgtatgatc cggtgaggca tcatcggtaa
     4801 taatcagttc ccaatcttga aaggtctggt cgagcacact ttgaatggct tcaccaacga
     4861 attgttgatg attgtacgcc gccatacata tacttatttt cggcatgtaa ttccctccag
     4921 caatttcggg gatgagtcac tgtagctcaa agcccgaaaa tcgatcgata atttttaaaa
     4981 ttcgttaagt aagctgtgat tcctgctttt tcatggaact taaccgaagt tcatggaaaa
     5041 cccgcgagtc aagaaatagt tcacaatccc gacggattca ttcgtttaat tgcaatattc
     5101 atttacatat caagtataga gaagcttttc acgggtcttt tcagacaaga tataatgatt
     5161 gttaagaaat agggaatagt aatctctatt tggagctagt cattttgttt ataaaccctt
     5221 tcagaatcaa ccttaccatt gatacgacgt ttcttttccg gttttttaat cttaccgccg
     5281 cggtttcgaa atatgcaaaa gatcaggagc atcaaatgga aacaccggtt caaccaggcc
     5341 aaaaggtaca attggaaatc accagttacg gccatgaggg tgaaggcgtt gggcgttatc
     5401 aaaactttac tctttttatc ccgggcgcgt taaaaggtga agccgtggaa gccaaagtaa
     5461 cagagatcaa aaaaaacttt gcccgcggca ggttactcag gataatcgag gagattccgg
     5521 agcgaagaat accggcatgt ccggtctatc atgaatgtgg cggttgtcaa ttgcagcatc
     5581 tcagctaccc cgcacagctg gcgctgaaac ggcaacaggt gatggacgcg gtagaacgta
     5641 tcggtggtct gaccggggtg acggtccatc ccacccttgg tatgaaggaa ccccgttgtt
     5701 acagaaataa ggtccagtat cccctggcaa tggagaatgg gaaagtgagt gccggatttt
     5761 atcggaaagg tacccatcaa atcgtcccgg ttaccggctg tttgctccat tcggaactga
     5821 gcaaccggat tgttgctgca gttgttcaaa agatagagga gaatcatgtt ccgatttata
     5881 acgagcgaac cggttccggg ctgttacggc acgttttgat caagaatgcc ttcgctaccg
     5941 gggagatgat ggtggtgcta gtgaccaatg gcgagaagtt tcccagtggt tcgaagatag
     6001 caacagagct tatgacagaa tttccggcga ttcggagcgt agttcagaat atcaaccgct
     6061 cccggggcaa tgtaattctg ggaaaccaat cggtggttct tggcggcagt gatgcgattc
     6121 aggatattct cggcggtttg aagttcaaga tttcggcacg ttcttttttt caggtgaatc
     6181 cggttcaaac tgaggttttg tatgaaaaag ccgtggatta tgccggtctg gcgggaacgg
     6241 agaccgtgct cgacgcttat tgtggagtcg ggagtctgac attgtttctg gcgcggagag
     6301 cccgggcggt ttacgggatc gaggtggttc ccgaggcgat tcgtgacgcc cgggagaatg
     6361 ccgcgttaaa tgaaatgaag aatgtacaat tcctggttgg ggagaccgaa agggttctgc
     6421 cggaattggc acggcaagga atccatttcg acgtagcaac agtagacccg ccccgggccg
     6481 gttgcgaacc ggagctactc aggagcctgg ccgtgaatgg ggtagggagg atcgtctacg
     6541 tcagttgcaa ccccagcacc ctggcgcggg atctaaagtt gttgggggag ttggggtata
     6601 agacgatgga aatacaaccg gtggatatgt ttccccagac gtatcacgtg gaaacagtag
     6661 tgttgatgtc acgtaaagat aaataaatcg gttctaaagg gcttgaaatc aaaggtttcc
     6721 agactttcgg taaaaataac cgattgctgg aaaccttttt tatcctccga gtatagcttg
     6781 gaacataaaa tataagtcag ggttgagact acagaactgc tttttgcctt gcgagacaag
     6841 gaaactactg tttttaagat aggttgggac tagagaactg ttgttaatgc tagaaaaaaa
     6901 tgatgagttt tgaatattat cttgacaagt gaacgattga taactataat acaaagtgaa
     6961 caatcgataa cttgcaacta aataaattat gaggtgaatg atatgccgaa gacagataca
     7021 aagagccgga tactgacgga gtgccttgca cttttttcaa aacatggtta tgatggagtt
     7081 tccatgcgag atattgcaaa agcaattggt tttcgtgaga gtgcgttgta caaacactat
     7141 aaaagtaagc aagagattct ggattctatt tttagtgtca tgaaagctca ctttgaagaa
     7201 gcatcaaaag caatcagctt atcagatggc gatgattttt ctgtgcttgc acatggatat
     7261 aaaaatgagg gcttggatgt tctaaaaaaa cagtgttctg ttctatttct atactggctc
     7321 aaagacgaac aggcagcaca gtaccgtaga ctgctgatgt tggaacagtt tcgtcactcc
     7381 gaagcggaaa acttgctacg ggagttcttg atggacggtg cactgcaata tcaagctgct
     7441 ttatttaaga aaatgattga gataggctat ttcaaaaaga ttgattcgga agtgttggcg
     7501 ctgcaatttt actcccccat tttcatgctg ctttgtaaat atgatagtct accggataag
     7561 gaaagcgaag cactgtccat tttagaacgg catgtcgctt catttgacgc cgcctatagg
     7621 aacgaggacg taaaatgaaa ctgttatgag tttcattaac cacggaatga ttatatagaa
     7681 atcgaacgct ttgaagcggt tatagaagaa atattgatga ggaggtgtat gccaatgata
     7741 tgccatgtga acaacaccaa tctttactac gaaaaatctg gcacaggaaa accactgctt
     7801 ctgattcatg gaaacgggga aacacataag atttttgata aggcgatgcc cttactggca
     7861 gaatgcttta ccgtatacgc aatagacagc cgtgggcatg ggcagagtga ggctgtttta
     7921 gtatatcact atgatgattt tgctgaggat atcaagtgtt ttattgaaga gctgaagctg
     7981 gaacaaccca ttctttatgg attcagtgat ggcgggattg tgggactgct gcttgcagca
     8041 aagtatccac aattattgtc ccaaatcata atcagcgggg ccaatttaga tccgaaggga
     8101 atacgtacag gttggcttaa gctgtttcag tgtatcaacg caatcgtgaa agacccaaag
     8161 atgaccatga tgttgaaaga acccaatttt acagctgaaa tgcttagtca tattgcaatc
     8221 cccactacgg tactggcggg aagtcgtgat atggtgaaac ggagccacac acagtacata
     8281 gctgataaca tcccaaacag cagactccac attttgcggg gcgagggaca tggtagctat
     8341 gttctgcaca gtactaaaat tgtaaagatg attttagatg caacaaatta gaaagagtaa
     8401 gatagcgctt tattgagtgg tatggcttaa aggtttatta catagtcaat cctgttgaca
     8461 tgatcagaga aattctgatt gcaaatagaa aagtgcatac atatggaggt gtttgttaat
     8521 gttaaattca aaaatgttct atgaagatat tgaaaaaaaa tttatttctt ggtcacaaac
     8581 ggttgaagac ataagggcag catttatcgt cggctctcgt gctcgtaatg accattacac
     8641 cgattgtaat gcaatgcttg tcggaacggg ggacgttcct tctgcgctta gcttttatca
     8701 aaaatgtgga ttctcagaat cacatcgaat atataacttt ttcacggata attatgacca
     8761 tccgatgttt gaagacggga aacagcttgt tgatatggtt tatctgaaac gggaacgata
     8821 aattacaagt agcaaaagca caatccaaga tattagtgaa tgtgagacaa gggattccgg
     8881 acgctgtgat gagtcgctca tcgctgaaac gcaattcaaa catgtctgac acctcgcagg
     8941 tgaaagctct tttttgcaga tattcttgat ttgacgagcc tttccctgca ctttaaggct
     9001 atttgttcac gatttcagga taatattaaa ggcctaattc tgatgctttt gttttttacc
     9061 agacacaatt atctaaagtc agtgagatat tgttttgagt gtaaaacata aaacagcctc
     9121 ttgacaatgg atatttctgg gcatacaatt aaatcaaacg attgattgaa ttgcaaataa
     9181 caacaatttt ccatcaatcg tatgaaaata acgaatatga caggaggtgt tgaaatgaag
     9241 ctttacatta tacagaccgg ttatacgaaa gttccatacg gacaattttt cggaggactg
     9301 gagggatgga gcggcttaaa ggggaagatc aaatttgcca ccgataagaa gcattttatc
     9361 ttagtgcctg ttaactgctt tcttattgaa catccgtctg agggaccgct cctggtggat
     9421 acgggtatca atcgggaaca agctcgaaaa cataaagaat actataccgc attgatggga
     9481 cttgtgacgg atgatgatga atatgtaatg cagccggaag aggaaataaa ttcacagctg
     9541 catgcaatag gatataaatg cagtgatgtt aagaaaatat tattgacgca tcttcatgaa
     9601 gatcatatag gtggtttaaa acattttccc catgccaaag tctttgtcag ctcgcaagag
     9661 tggctgttaa aggataagaa aattttgggg ctgattcctg gagtataccc gaaatctata
     9721 gctccgatca ctgattggga agtgatatct tattcatccg gggcatttta caacttcgcg
     9781 aaaagccaag atatttttgg agacggcagt attattttgc tcccgacgcc tggacatacc
     9841 agcgggcata caagtgtttt cttacaattt ggtgattatc agatactgct ttgcggagac
     9901 tgtttgtata ctcttcgcca tttggctgtt gaccaggtca gatccgttat tattaattca
     9961 gaaatgactg agaattacat ccggtcaata cgattaacac aggaactaca agaggcgtta
    10021 cctaatttgg ttattattcc cggacatgat cctaccgaat atcacgagaa gtatttaaag
    10081 caatttttta aggatggaat tttgtctgca gaagaactgg ctgttgtaaa taaatacgaa
    10141 aaacagcttt ttgaaagcgg attccacttg gcgaaaaaat ctatgccgca atttatgccg
    10201 gttcccaata acggatatgt tggaacggtt agctaatttt ttcaattaaa tcaatcaaaa
    10261 gattgattga taattatcat aatataatat agaggtgcaa ttatgtcaag tattgggatg
    10321 tctaaagcta ttgaagaagc caaaagaatg caggagattg ttccgtgaag agttaaaagt
    10381 gtttgtaccc gatgcatcag gtattcatgc tgatgttgtc attggacgca aaatgtaatc
    10441 cctgttaaaa acaatttgaa ataaggagta ttgtaatata caatcaaatc aaacatttga
    10501 ttgaggtggt tttaatagag actattgtcg attcaaaagc aaaaatattg actgctgcaa
    10561 aagagctgtt tgcgagaaag ggctttgaag gaacatcaac cagagaaatc gtaaatgctg
    10621 ccggagtaaa tatctccttg atagcctatc atttcggcgg aaaggaaaat gtattatttg
    10681 cactatttga tcatttcatg gatggagctt atcatgaaga cgtctctttt ggcgattctg
    10741 aactggttga agagtttaag tccatattga ttcgtattat aaaactaaga tttgaagaac
    10801 cggaattaat cagcatacta catcatgaaa tcattttgaa cagtggaaga accgataaaa
    10861 tcaaagcctc cctgataccg gtctggacaa gagtagaatt gttactagac gaaggaaaaa
    10921 gaagaggact gttcagtttc gaaaatacaa aaaatg
//
LOCUS       DASVMB010000342         7283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_631578,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000342 DASVMB010000000
VERSION     DASVMB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631578"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..787
                     /locus_tag="VF531_09745"
     CDS             98..787
                     /locus_tag="VF531_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714294.1"
                     /translation="MIPKNQYRIYLVEDDRMLAEEIQKLLMKWGYDVHIAKMFDAIPD
                     EFTTIQPHVVLMDVNIPSFDGFHWCKKIREISSVPIIYISSRDGSMDMIMGMNNGGDE
                     YVTKPFDNAVLVAKLQAIIRRTYEYCSTDPRVLRCRGLTVNLEEATAAYENRKYELTK
                     NELKILKLLMENEGKIVTRQAFMRDLWDDEIYVNENTLTANVNRVRLRLEDLGLKGFI
                     TTKKGMGYMII"
     gene            784..1857
                     /locus_tag="VF531_09750"
     CDS             784..1857
                     /locus_tag="VF531_09750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7714295.1"
                     /translation="MKTTAYIREQWEWITSQAGIVILINLILLTSAPLDKSLGEIFYM
                     DLLVFFVAGAGCIAHFCRLNKAYTKIRQTMAEAGNVDGVLGEGFAVAAKNVPGISLMK
                     ELLEYENRSFNQKENDYQQRMNNLKDYLTQSIHDLKVNLSICEMVVNRLENETDIANK
                     LLFQIEQMKFRINQILSIARANHYSEDIVSEKVDLERVVKEAIGDNAEFFINKGIRIR
                     NDLKQYHFISDKKWIHYIVTQILNNSSKYTGKDGEITIFSREDEKGYYLHLKDNGIGI
                     PEEELDRVFDKGFTGSNGRDNTKSTGMGMYYAKKMANVLGIGMTVQSKRGVYTEFILS
                     FYKLSDYMRRQIGQNDKNVTYNS"
     gene            1938..2702
                     /locus_tag="VF531_09755"
     CDS             1938..2702
                     /locus_tag="VF531_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714296.1"
                     /translation="MTPVIDVKNLTKLYGGPGAVCRALYQVNLEIGTGEFVAVMGPSG
                     SGKTTLLNIISTIDKPSQGQIIIDGRETENLKEKELALLRRDVIGFVFQEFNLLNNMT
                     IRDNIALPLTLNNVKTEEILRKVQELTALLGIDSQLDKYPYQLSGGQKQRAAVCRALI
                     NEPKILFADEPTGALDSKAAAELLTCFRNVRNQYGTTVVMVTHDASAASYCDRVLFLK
                     DGMISGKLETNGDSEAFFKKILNMVALLGGERNEHL"
     gene            2689..4629
                     /locus_tag="VF531_09760"
     CDS             2689..4629
                     /locus_tag="VF531_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714297.1"
                     /translation="MNTFKISWLLFKNNLKLYRFYLAVLIMTTAIYYNFLAVNFNPYM
                     QVLNEQYSFAQVASYLCSFILLLTVVSFMFHANNFFYKLRYKEIGTYMLMGIPGGKIG
                     TVFALESVMLGGTAIIIGLPVGLLFSKLFFMLLSKAMILDTQIPFYIPMKAITMLMII
                     LGTIILLMGLKNYRMVRRSRLIEILNAAQKEQSLPKIKWIRGLIGVVCIVLAYYLALN
                     ILDLEIDFIQATLFILVLICSGTYLFFGSFLAIILNMLIQKKKVIYKGSRLVSFSNTL
                     FRLGTHYRSLSMTAILCAATFTAFSGSMALKYFADTNTAVEAPYSISFINQDGATNQK
                     IKALIQESPHHILAEHQSHFITGKVTYNNGSGEHTETCLITSYSEVRKSLMVTQPGNY
                     RTILKKIAPQDNEAVSILHSNLIFSGISHIREVHVIQGKPYILKREVKIPFIGEIEDI
                     GKYQTYIVTDRQYSELKKNISEMTLYGINFTNPEDSIVLVEQIARVMKNPRGNLNSYA
                     GQYQYKYYLIGAFYFMGLVMAIVFVVSTFSTIYFKILSDAILDREQYWILMKIGMTER
                     EIAESIHTQVGMAFILPAVLGMAHGIMAIKALESFIHYQFTESILVGVGVLILAMAGF
                     YSLMGRKYQDMVVKGWGSHETA"
     gene            4616..5884
                     /locus_tag="VF531_09765"
     CDS             4616..5884
                     /locus_tag="VF531_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEX7714298.1"
                     /translation="MKQLDFKMKIHAKAGFRLMAVFIVLMLTGCGIIPSTEPYLAKER
                     DSGWVNDITYLQKTLPQVHKNLYFHLSEKEFNRQLEKLKNNVPHETDEQIEIALSVIL
                     AGIGDTHTGSSIGSEFRYPLELHWYAEGIYIVGTAKEYPNLLDAKIITLNGKKIEEAV
                     NILRPLLAGANESWFKTQIVYYLPIPGVLKYFGLSNSDEIELRVELKNGLVEKVKLKS
                     MNYKEYVAADRPDKTVPLYRSHPYENYWYEYLKKEKITYLNYNSCRQMRNKPFEIFDR
                     ELWNFINTHEIDKLILDIRENRGGISTILDPFIKELKNSSFNRQGELYVIIGKDTYSS
                     AILNAIRLKKETNACFVGEATGGEPNHYGEVREFKLPNSQKAIRYSTKYFHWLDQDIN
                     TFEPDKEIPETFAAYRDGTDPVLEWIVKQN"
     gene            complement(6002..6394)
                     /locus_tag="VF531_09770"
     CDS             complement(6002..6394)
                     /locus_tag="VF531_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714299.1"
                     /translation="MMVKKTDKDEKVKTTPYSPPAGKIREAGYGPSTDFEIADLDTNQ
                     KNQEKTGFNFGNSAQEPGQNTEFGNLESNQPYQNNNMNFAFGHKDNDNFEFGESNQEK
                     YQAGYSQELNGLKTNRGKDTNHTKEKNT"
BASE COUNT         2179 a         1413 c         1761 g         1930 t
ORIGIN      
        1 ccgtctgatt aaatggcaat aatattatag ggaatgtgac aactcataaa ttatgataaa
       61 actggaatag agcaaaaaat gaatgaggca taaaaggatg ataccaaaaa accaataccg
      121 aatctacctt gtggaagatg accggatgtt ggctgaagaa atccaaaaac tcttgatgaa
      181 gtggggttat gatgtccata ttgcgaaaat gtttgatgcc atcccggatg aattcacaac
      241 catccagccg catgttgttt taatggatgt caatatccca agctttgacg gctttcattg
      301 gtgtaaaaaa atccgggaaa tctccagtgt tccaatcatt tatatctctt ccagggacgg
      361 cagtatggac atgatcatgg ggatgaataa cggcggcgac gaatatgtaa cgaagccttt
      421 tgacaatgct gttttggtgg ccaaacttca ggcaattatc cgcagaacat atgaatattg
      481 ttccaccgat ccccgtgttc tcagatgcag gggacttact gtcaatcttg aggaagccac
      541 tgcggcttat gaaaacagaa aatacgaatt gacaaagaac gaactgaaaa ttctaaaact
      601 attgatggaa aatgaaggga aaatagtgac tagacaagct ttcatgaggg acctatggga
      661 tgatgagatc tatgtgaatg aaaatacctt aaccgccaat gtaaaccgtg tgcggctgag
      721 gttggaggat ctagggctta agggctttat tactactaaa aaaggaatgg ggtatatgat
      781 catatgaaga caacggcata tatcagggaa caatgggaat ggattaccag ccaggccggg
      841 atcgtgatcc tgatcaattt gattttactg acaagtgctc ctctggataa aagtctgggg
      901 gagatttttt atatggattt actggtgttc tttgtagccg gagcaggatg tatcgcacat
      961 ttttgccggc tgaacaaagc ctatacgaaa atcaggcaaa caatggccga agccgggaac
     1021 gttgacgggg ttttagggga gggatttgcc gtagcggcaa aaaatgtccc gggaattagc
     1081 ttaatgaaag aactgttgga atatgaaaac cgaagcttca accaaaaaga aaacgactat
     1141 cagcagcgaa tgaacaattt aaaggactat ctgacccagt ccatccatga tttgaaggtg
     1201 aacctttcca tatgcgaaat ggttgtaaac cggttggaaa atgagacgga tatcgcaaac
     1261 aaactattat ttcagattga acagatgaaa ttccggatca accagatctt atccatcgcc
     1321 agagccaatc attacagtga ggatatcgta tcggaaaaag tagatttgga acgggttgtc
     1381 aaagaggcga ttggcgataa tgcggaattc tttattaata agggcattcg tatccggaac
     1441 gatttaaagc agtatcattt tatcagcgat aaaaagtgga ttcattatat tgtaactcaa
     1501 attctcaaca atagcagcaa gtacactgga aaagacggtg aaattaccat attcagccgg
     1561 gaagatgaga agggatatta cttacaccta aaagacaatg gaatcggtat tccggaagag
     1621 gaactcgatc gagtctttga taaaggattt accggcagca acggcagaga caataccaaa
     1681 tcaaccggta tgggcatgta ttatgccaaa aaaatggcca atgttttggg tatcggaatg
     1741 acggtacaaa gtaaaagagg cgtatatacg gagtttattt tgtcttttta taaactgtcc
     1801 gattatatgc ggcggcagat aggccagaat gacaaaaatg tcacatacaa ctcttaaatg
     1861 tcaccttaat cggtagaacc ggagcggaag agattctata ataatgtcat accagaagac
     1921 gacaaggagg aaggcgaatg actccggtta ttgatgtaaa gaatttgact aaactctatg
     1981 gcgggccggg cgctgtttgc cgggcgcttt atcaggtgaa tttggagatt ggcacgggcg
     2041 agtttgttgc cgttatgggc ccgtccggtt ccgggaaaac gaccttactg aatatcatct
     2101 ccaccattga taagccttcc caagggcaaa taattattga cggcagagag acagaaaatt
     2161 taaaagaaaa agagctggcc ttgttgcgta gggatgtcat tggatttgtc tttcaggaat
     2221 ttaatttgtt aaataacatg actatccggg ataatattgc tttgcccctt acgctgaata
     2281 atgtaaagac ggaagagatt ttgcgcaaag ttcaggagct gacggcgctg ctgggtatcg
     2341 acagccagtt ggacaaatat ccataccagc tctccggcgg gcaaaaacaa agggccgctg
     2401 tctgccgggc tctgatcaac gaacccaaaa tattgtttgc cgacgagccc accggggcac
     2461 tggattctaa agcagctgcc gaacttttga cctgtttccg gaatgtcagg aatcaatatg
     2521 gaacgacggt ggtgatggtg acgcatgatg cgtctgccgc tagttattgt gaccgggttt
     2581 tgttcctgaa agacgggatg atcagcggta aattggagac aaacggagac agcgaagcat
     2641 tctttaaaaa gattttgaac atggtggccc tgctcggagg tgaaaggaat gaacaccttt
     2701 aaaatctcct ggctgctttt taagaacaac ctaaagctct atcgattcta cctcgcagta
     2761 ttaattatga ctaccgcaat ttattataat tttttagcgg tcaattttaa cccgtatatg
     2821 caggttctca atgaacaata ctcctttgcg caagtcgcaa gttatttatg cagtttcatt
     2881 ttacttttga ccgtggtttc ttttatgttt catgccaata atttcttcta caagctgcgt
     2941 tacaaagaaa ttggaactta tatgttgatg ggcattcccg gcggtaagat cggtacggta
     3001 tttgccctgg aaagtgtcat gctgggagga acagccatca tcattggcct gcccgtcgga
     3061 ttgctctttt ccaaactttt ttttatgctg ctgagcaaag cgatgattct ggatacccag
     3121 attccgtttt atatcccgat gaaggccatt acaatgctga tgatcatctt gggtacgatt
     3181 attcttttga tgggcttaaa gaattatcgg atggttcgta gaagcaggct gatagaaatc
     3241 ttaaatgctg cgcagaaaga acaaagcctg ccgaagatca aatggattcg cggcctaatc
     3301 ggtgtggttt gtatcgttct cgcttactat ctggcattga atattcttga cttggaaata
     3361 gattttattc aagcgacgct tttcattctg gtactgattt gcagcggcac ttatctcttc
     3421 ttcggcagct ttttggcgat catcctcaat atgctgatac agaagaaaaa agtcatatat
     3481 aaaggatcca ggctggtaag cttcagcaat accctattcc ggctgggaac gcattaccgg
     3541 agcctgtcca tgacggctat cctgtgcgcg gctaccttta ctgctttcag cggcagcatg
     3601 gctttaaaat attttgcaga taccaatacg gctgtcgaag caccttatag tataagtttt
     3661 atcaatcagg atggggcaac gaaccaaaag attaaagcat tgattcaaga atctccccat
     3721 catattctgg ccgaacacca atcacatttc ataaccggca aggttaccta taacaatggt
     3781 tccggagagc atacggaaac ttgcctcatc acaagttatt cggaggtacg gaaatcatta
     3841 atggtaaccc aacccggaaa ttatcggacg atcctaaaga agattgcacc tcaggataat
     3901 gaagcggtaa gcattttgca ttcgaacctg attttttcag gtatcagtca tataagagag
     3961 gttcatgtta tccaaggtaa accctatatt ttaaaaagag aagtcaaaat tccgtttatc
     4021 ggtgaaatag aggatattgg caaatatcaa acctatattg tcacggatcg acaatatagt
     4081 gagttaaaaa agaatatcag tgaaatgacc ctgtacggga tcaatttcac gaatccggaa
     4141 gacagcattg tattggtgga gcaaattgca agggtaatga aaaatccgag gggcaattta
     4201 aacagttatg ccggacagta ccaatacaag tactatctga tcggggcctt ctattttatg
     4261 ggtctggtga tggccattgt ttttgtagtt tctaccttta gcacgattta ttttaagatt
     4321 ttaagtgatg ccattttgga tcgggaacag tattggatcc tgatgaaaat agggatgaca
     4381 gaacgggaga tcgccgaaag catccatact caggtaggca tggcctttat tctgccggca
     4441 gtattgggaa tggcccatgg cattatggcg attaaagccc tggagtcatt tatccactat
     4501 cagttcactg agagtatcct ggtgggggtt ggcgttttga tactcgccat ggcgggtttc
     4561 tattcgctaa tgggcagaaa ataccaagat atggtggtca aggggtgggg cagtcatgaa
     4621 acagcttgat tttaaaatga aaatacatgc caaagcaggc ttccggctca tggccgtttt
     4681 tattgtgctt atgttaacag ggtgcggcat tatcccttca actgagcctt acctggcaaa
     4741 agagagagac agtggctggg tgaatgatat cacctatctc caaaaaacgc tgccgcaagt
     4801 tcataagaac ttatatttcc atctctccga gaaagaattt aaccggcaac tggaaaaact
     4861 gaagaataac gtcccccatg aaacggatga acaaatcgag atcgcgttga gcgttattct
     4921 ggccggcatc ggcgatactc ataccggatc aagcattgga tcggaatttc gatatccgct
     4981 ggagcttcat tggtatgccg agggcattta tattgtcggt accgccaaag agtatccgaa
     5041 cttgttggat gcaaaaatca ttacattaaa cggtaagaaa atagaagaag ccgtcaatat
     5101 actcagacct ctacttgccg gagccaatga aagctggttt aaaacccaga tcgtttatta
     5161 tctgccgatt ccgggcgtcc tgaaatactt tggtttgagc aattcggacg agatcgagct
     5221 gagggtggaa ttgaaaaacg gactagtgga aaaggtgaaa cttaaatcca tgaattataa
     5281 ggagtatgtg gcggctgacc ggccggataa gactgttccg ctgtaccgga gccatccgta
     5341 tgaaaactac tggtacgagt atttgaagaa agaaaagatt acatatctga attacaacag
     5401 ttgccggcag atgagaaaca agccctttga aattttcgac agggagttgt ggaattttat
     5461 caacacccat gaaattgata agcttatctt ggatatcagg gaaaatcggg gcgggatctc
     5521 gaccattctg gaccctttta tcaaggaact taaaaacagc agtttcaatc ggcaaggtga
     5581 gctttatgta atcataggca aagatactta ttcttccgcc attttaaatg ccatccgttt
     5641 aaagaaagaa acaaacgcct gttttgtggg agaggctacc ggaggggaac cgaaccacta
     5701 cggagaggta agggaattca agcttccgaa cagtcaaaaa gcgattcgtt attctacaaa
     5761 gtatttccat tggttggacc aagatatcaa tacgtttgaa ccggataagg agattccaga
     5821 aacttttgcc gcataccgtg atgggactga tccggttttg gagtggatag tgaaacagaa
     5881 ttgagatgga agcagattta catataaggt ccgccgtgat tagccggtag gaatccattc
     5941 acctaccggc tttttctaaa cggtggaact atcttgattt tttaactact gaggtatgca
     6001 attatgtatt tttttctttg gtatggttgg tatccttgcc acgattggtt tttagcccgt
     6061 ttaattcctg agaatacccg gcttggtact tttcttgatt tgactctcca aattcaaaat
     6121 tgtcgttatc cttatgtccg aaggcgaagt tcatgttgtt gttttggtag ggttgattac
     6181 tctccaagtt cccgaactcc gtgttttggc cgggttcttg ggcggaattg ccaaaattaa
     6241 aaccagtctt ttcttggttc ttttgattag tatctaaatc agctatctcg aaatcggtac
     6301 tcgggccata acctgcctct ctgattttac cggccggggg ggagtaggga gtagttttaa
     6361 ctttttcatc tttatcggtc tttttcacca tcattgaatc ctccattttt tttaagtttg
     6421 ggtatatctt tttgtggcta ccgttctaaa ttattatggc ctgattcatt aataaaatag
     6481 agggataaac ggagccataa accaggatgt tccagatacc ctatctgttc tttacaaggg
     6541 tacaaacgat aaaaaaaccg gaacggacta ccaacttaac ataagcgccg cccactggac
     6601 aaggggcggc tctttgttag tacgcccggc atgaacgttg tctatagggt gaaagtcccg
     6661 aacggaggct ggtgagcgcc taccgttagc cgagggcaag ggtgtctacc gcgagcgcgc
     6721 cttccgtgaa ggcgaagaat ctgaaggaag ccgaaggcaa atgctcgacc taaggaacac
     6781 gaacccaatc tgaggctggg atagccggat gagcctgcta aacaagcgcg aagtccaaag
     6841 ccgccaagag ctatcaaagt aaacttgggt aggtagatga gcagaaagtc aacgttctta
     6901 tccggggagg tctgcgtgat aagccgtctg aagatggtaa ccgaggttga gaagccttgt
     6961 tgaacacgca gaattcagcc gaggtcatag taccaaagta ggagacgtcc gaaaagggaa
     7021 gggccgaacg acaattaggg atgaaatcgg aaactttcga gagcagcgta aagaggcaga
     7081 attccgaaag gaactgttcc ggtagagtac aggtgaagcc tggagggaac cggaaagggc
     7141 cgagcgctga ccggcaagac caaggacatc ccgcgaagga gaagccttta atggacttga
     7201 tggaacaaat gctcgcacgg gataacctcg ttaccgccct gaaaagggtg gaggcaaacc
     7261 ggggcgcagc gggaatcgac gga
//
LOCUS       DASVMB010000343         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_118145,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000343 DASVMB010000000
VERSION     DASVMB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118145"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VF531_09775"
     CDS             <1..1091
                     /locus_tag="VF531_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714300.1"
                     /translation="DMISETAQKGITDIESLVAELAPPDQDRFRRFFRIDTTVGELRA
                     PESMAGWINKNFGSVDRVASQKIVKITNLLSLEGALCNEVRASRPMDCKVNDEVRHII
                     ENSVGDPFCKPAEGTPADKFGRVEGKHSITASNIAKYDGYHGLVIFEDHNPLAINREA
                     IRDYFETGFEWAHRAHQEDPEAKYYFFMWNCLWKSGASIIHGHAQMTLTRGMHYPRVE
                     ALRRIFRDYQQTYGNCYFGDLHQVHESLGLGWRWDGGVKGFAYLAPIKEKEVLLFAPE
                     PGPPLYDAVHRVLEKYMALCSISFNLALYVPPLAQSEEDWSGFPAVVRIVDRGDPNNK
                     TADVGAMELYAASVVSSDPFRVAEFMNS"
     gene            complement(1131..1346)
                     /locus_tag="VF531_09780"
     CDS             complement(1131..1346)
                     /locus_tag="VF531_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714301.1"
                     /translation="MAKKLSVLVSDELYNQLIQIKGTDEVQSTMEHVRSALRLYIWYR
                     QQTDSGFSVFAKKEEGDKTILREIILQ"
     gene            1447..1659
                     /locus_tag="VF531_09785"
     CDS             1447..1659
                     /locus_tag="VF531_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714302.1"
                     /translation="MAKKVTLILPDDVFKHLSEVAVTSERTLSATAVAGIESLYWMRK
                     QKEEGFVVRAEKEERDKVIIREISVP"
     gene            1656..1991
                     /locus_tag="VF531_09790"
     CDS             1656..1991
                     /locus_tag="VF531_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714303.1"
                     /translation="MKDEIQKVQVETGETVEDIPEEQYEGYHVKEGYVLTQTEKSIAK
                     NKAKMANFIGCLIIGGFIFSIVVNLGCIVFKVIPTNEAVEIMKTLTNTFGAPLGFVLG
                     YYYGVMKSE"
BASE COUNT          525 a          409 c          543 g          533 t
ORIGIN      
        1 aagatatgat tagcgagacg gctcaaaaag ggattaccga catcgagtcg ctggtggcgg
       61 agttggctcc acccgatcag gatagattcc ggcgtttctt tcgtattgac accacggttg
      121 gggagctccg ggctcccgaa agtatggcag gctggatcaa taaaaatttt ggttcggtgg
      181 accgtgtggc cagccaaaag attgtcaaaa tcaccaatct tttaagcctg gaaggtgcgc
      241 tgtgtaacga agtgcgcgcc agccggccta tggattgcaa ggttaacgat gaagtgcgcc
      301 atatcattga gaacagtgtc ggagatccct tttgcaaacc cgctgaaggc actccggccg
      361 ataaattcgg ccgggtagag gggaaacatt ccattaccgc aagcaatatc gccaagtacg
      421 acggttacca cgggctggtg atctttgagg atcataaccc acttgcgata aaccgcgaag
      481 caatccggga ttattttgaa accggttttg aatgggctca tcgtgctcat caggaagatc
      541 cggaggcaaa gtattacttt tttatgtgga actgtctatg gaagagcggt gcctcgatca
      601 ttcacggtca cgcccagatg acattgaccc gggggatgca ttatcccagg gtcgaggcct
      661 tgcggcgcat ttttcgagat tatcagcaaa catatggcaa ttgttatttt ggcgacctgc
      721 accaggttca tgagagtctt ggattgggtt ggcgctggga tggaggggtc aaggggtttg
      781 cctatcttgc tccaatcaag gagaaggagg tgctgctctt tgctccggaa ccgggaccgc
      841 cgctttatga tgcggttcac cgggttttgg agaagtatat ggcgctttgt tccatcagct
      901 ttaatctggc gctttatgtt ccgccgctcg ctcaatcgga agaggactgg tccggttttc
      961 cggcagtggt gcgaattgtg gaccggggag atcccaacaa caaaactgcc gatgtcggag
     1021 ccatggaact ttacgctgcg agcgtggttt caagtgatcc attccgggtc gcggaattta
     1081 tgaattctta atgtgagaag ttaaggtgcc gcggattttc gcccgcggca ttattgtaaa
     1141 ataatctccc tcaaaattgt tttatcgccc tcctcttttt tcgcaaaaac agaaaatccg
     1201 gaatcggttt gttggcgata ccagatatat aagcgcagtg cggaacgaac atgctccatg
     1261 gttgattgaa cctcatcggt gccttttatt tggatcagct gattgtaaag ctcatcggat
     1321 acgaggaccg atagtttttt tgccaagagg ctcgccacct ttcctattca ttgtcggtat
     1381 attctacgta atttatacgt tgaaattacg tagaatatgc gttaaaattg ggtaggaggg
     1441 atccatatgg cgaaaaaagt aactttgatt ttaccggatg atgttttcaa acatctaagc
     1501 gaagtggcgg taaccagtga gcgcacgctt tcggcaacag cagtcgccgg tatcgagtct
     1561 ttatattgga tgcgcaagca aaaggaggag ggatttgtgg tgagggctga aaaggaggaa
     1621 cgcgataagg tgattataag ggagatctct gtaccatgaa ggatgagatt caaaaagtcc
     1681 aagttgaaac gggggaaaca gtcgaggata tcccggagga acagtatgaa ggctatcatg
     1741 taaaagaggg ttatgtcctc acacagacag aaaaaagcat tgccaagaat aaggccaaaa
     1801 tggccaattt tataggctgt ttgattatcg gcggtttcat tttcagcatc gtagtcaact
     1861 taggttgtat cgtctttaaa gttattccta ccaacgaggc agtggaaatc atgaagacat
     1921 tgacgaatac tttcggtgcg cccttaggat tcgttttagg ctattattat ggagtaatga
     1981 agtcggaata atcgaatttt ttaacgttca
//
LOCUS       DASVMB010000344         8631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_631773,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000344 DASVMB010000000
VERSION     DASVMB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631773"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VF531_09795"
     CDS             <1..681
                     /locus_tag="VF531_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7714304.1"
                     /translation="GLQPAVAKNIVSYREENGRFRNRNQLKEVKRLGDQAFVQAAGFL
                     KIGDGDDPLDATSVHPESYPAARQLLQELGFSTADLLDKERLTWLRTKIPLVNIEKTA
                     TKLGLGTPTLKDIIAALTKPGRDPREDLPKPIFRNDVLAMEDLKPGMILTGTVRNVVD
                     FGAFVDIGVKQDGLVHISQLSDRYVKDPTEVVAVGDVVEVRVLEVDQTRKRIALSMKK
                     DSAPKAEV"
     gene            925..2682
                     /locus_tag="VF531_09800"
     CDS             925..2682
                     /locus_tag="VF531_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7714305.1"
                     /translation="MKIRLQYKIIVTFIGLLLCCGGVIFLIADTEISRVITLQETEQF
                     NTLINTMYPGGWSRIGEELYKGTVLLNNNFSIVDTLKAQTRSIAAIFLGNVQITSNLR
                     LPNGQRMVGTKAPQTVVAQVLNRGREFIGEAKLNNRRYLVRYSPLKDSEGKIIGMWFA
                     GIEKSQLDRKTYQVNLKIALTILGVLIACIIVAFFLSKHILKAIPSLLAVFDRAARGD
                     LTVAAKIVTRDEMRTLSEGFNQMIRQQREMMIKVLNSAVQVKAAASQIAAVNQDLSQR
                     TQEQAATLQEVAATVEQVNSLAHHTSANSRQADELSRLMYQAAQGGGQSVAEALQAMD
                     GISGSSQKISDIIKVVNELAFQTNLLALNAAVEAARAGEQGRGFAVVAAEVRNLAGRT
                     AEAAREIERLIQGNGEQVEQGNQLVRQSAAALQEIIKQIEGTSKVIAEVASAMQEQAT
                     ATTQIQDSLAQLNQVTQQNAAIVEETAASSETLNAEAEVLHEMVSGFKVESEVDAASK
                     VQSETNPLRSSSSRLAPMGDTPSKQTPAMAIPLTNPQSPETQIPQESILQTLPRRRRP
                     EPRPLSGKPEPSEDGWEKF"
     gene            complement(2717..3439)
                     /locus_tag="VF531_09805"
     CDS             complement(2717..3439)
                     /locus_tag="VF531_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7714306.1"
                     /translation="MIQLQQPKGMYDNEDKELFQSPCPPSHDFTSEDPWRILRIQGEM
                     VEGFDALSKIGPAVAIFGSARLRPDNPYYQATIDIATRLAAAGIAILSGGGPGIMEAA
                     NLGAFNVKGTSVGCSIQLPEEQQPNSYQTIALKFRYFFVRKLMFVKYAIAFIIMPGGF
                     GTMDELFEALTLVQTDKIDHFPIILYASSYWKGLCDWMSDQMLPAGCIGRHDLDLLRI
                     ADHPAEAARIVIENSREHGFIH"
     gene            3630..3848
                     /locus_tag="VF531_09810"
     CDS             3630..3848
                     /locus_tag="VF531_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HEX7714307.1"
                     /translation="MAKQDRKASTQNGKEKLKWETARELGLDDDLAAGGDELSVKEAG
                     KIGGQMVKKLIAEGEKQIVQEDSGEGNA"
     gene            4238..6001
                     /locus_tag="VF531_09815"
     CDS             4238..6001
                     /locus_tag="VF531_09815"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEX7714308.1"
                     /translation="MRHFLFKNRFPWVLLVGLLFLFIFTPFSWGKEKYFPLDKVKPGM
                     SGFGYTVFGGTKIEKFDIKVLAVLDSSADQGKLIMVRLSGKRLAANGGLASGMSGSPV
                     YIGSKLMGAISYGFENADPYLAMVTPIETMLKLLPDQGNSVSLSGINRLYLGQVVTAV
                     RKHSGLVGSFKPVPLATPVILSGMGQRGYEMVQRSLQPLNLQTVYASNFGGVKLAEGK
                     TNLRPGSAIGVQLIAGDYQAAAIGTVTLCDGPDFLAFGHPFTNRGTVDYLANEAFIYQ
                     TVKSQVLPFKLGAPIRAIGRIFQDRQAGIAGRLGELPSLIPVVVNVTDRDRKFSNQRR
                     FQVVANEQLYRDLIISGVTDAIDQTIDRIGSGTAQVRLVIAGAERDPLLTRENLFYGK
                     DIAVNCLKDLRQALDLISTNEFLAVPIRSIAVNVEVWNKQDSARIVSLALDTDPSKLK
                     PGSSFKLIARLHTYRGEDLTVPFQVKIPPDTIPGKLILSLSGGSKDLPDETEDSEKVR
                     TDEGLDGSGRIGSFQELLNNYSNAPLNNELVLDYVLPDSGTKSDASGKIGSIPPGLQL
                     KVASDYFVLGDARLTLEILAP"
     gene            6182..>8631
                     /locus_tag="VF531_09820"
     CDS             6182..>8631
                     /locus_tag="VF531_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714309.1"
                     /translation="MFLALFFAQYFLLSDQALTGKIRNALQASLRGNGLQLRIERVHL
                     SGLNEFTGSGLILSDAGSGRLFFAAEQVRLEFDALSFLSNYRHPEACIRKIRIIKPHL
                     VLERRTSGWNFERYFPQTGQVMQFKATLQLEKGLVEFGDFQYGNYRLSQVAGKIDLSS
                     YPAVFWNLKGLSNLGPASQWTSNGSMRLDQQGGRGSLTVRQGLAARAAKFLPRPFEYR
                     IRSGLANLRLRFGWNRQNFWIEDGRATIFEAGISLPWFSRPFRVEYLQADFSPAEFKL
                     ENGRIKYGDTSIKVRGRFDVKKATFDGEVSASRLVLTDLIGIAPTIKDFAPSGTAGLD
                     LRVNGPLSAPVWNGWLTLKQAAFTPVAGERVAGINGRLRIHDNDLAVENLQGTWHATD
                     IRVNGTVRNLLRPDLNLTIAASEFDLKNIPWAEKFPGLELKTGSGDWFTATISGGLNR
                     PTITGAIDFEQVTYQTIPINGFQFRFGLDPVLSTLKILDLQGSFGDGQLNAKGEIHYD
                     NTHLQWLITGSASGLSLTQFPLKRPLAFKGIIGAEAIFRGDWTFGTPFDMGKVFGTLS
                     SRQLDLNGVRLDEAEGIFSWDQNAFTIDTVQAKADSGRIFGYLTWRQDTLVVDINAEN
                     LRLQKFFPLGPKIPLDGLFEGSVTFEGPPSDLKGWINGTFTEATWDSRPLGDISGRLA
                     YNDQKLHIEELRAATPTGEFVATGQVDLTGDPMLQVNLTSEACDLSGVMKWLPLDPAL
                     KPEGIARINLDLKGALANPGLHGVIQLTNPGLMNIRLNRGEIEFEGDLRRITLARFEL
                     QRDRAVLQLKGDISLEQY"
BASE COUNT         2163 a         2017 c         2332 g         2119 t
ORIGIN      
        1 gggctgcaac cggctgtggc caagaacatc gtatcctacc gcgaggagaa cggccgtttc
       61 cggaaccgta atcaattgaa agaggtcaaa cggttgggag atcaagcttt cgtccaggca
      121 gccgggtttt tgaagatcgg cgatggagac gatcctttgg acgccacttc ggttcaccct
      181 gaatcgtatc cggctgcccg acaacttctt caagaacttg gtttttcaac cgccgacttg
      241 ctcgacaagg aacgcctgac ttggcttcgc actaaaatac cgttggtaaa tattgagaaa
      301 acagcaacta aactgggcct cggcacaccg accttaaaag atatcattgc cgcattgact
      361 aaaccgggtc gcgatcctcg cgaggatctg cccaaaccca tattccgcaa cgatgtgctg
      421 gcgatggagg acctcaaacc ggggatgatc ctgaccggaa cagtcagaaa cgtagtggat
      481 ttcggcgctt ttgtcgacat tggggtcaaa caggacgggt tggtgcatat ctcccaattg
      541 agcgatcgtt atgtcaagga ccccaccgaa gtagtggctg tcggagatgt cgttgaggtc
      601 agagtccttg aggtggatca gacccggaaa cggattgcac tctccatgaa aaaggattca
      661 gcccccaaag ccgaagtttg aatcactgtc aatacttgac tccatagacc attgactgaa
      721 agcaggacct gtacggtcct gttttttttt attatcaaac ttggattgat agttctttcg
      781 tggaataaat ccttatattg ccataaattg ttctccaggg gtcaataaaa atcgatttcc
      841 agccgatatg tatagtattg gtttatcctc cgctattcgg tactcccaaa aacacagaca
      901 acgtgaagct ttggagggat tattgtgaag atcaggttgc agtataaaat catcgttact
      961 ttcatcgggc ttctcttatg ctgcggtggg gtcatctttc tcatagccga taccgagatc
     1021 agccgtgtaa ttacactaca ggagaccgag cagttcaata ccctgattaa taccatgtat
     1081 cccggcgggt ggtctcggat cggggaagag ttgtataaag gaacggtctt acttaacaac
     1141 aatttcagta ttgtggatac ccttaaagct caaacccgtt ccattgctgc aatttttctg
     1201 gggaatgtcc agatcaccag taatctacga ctacctaacg gtcaaaggat ggtaggaacc
     1261 aaagccccac aaaccgtggt ggcgcaagtt ttaaatcgcg gccgggagtt tataggggaa
     1321 gcgaaactga ataaccggcg atatctggtt cgttattcac cgctcaagga tagtgaaggt
     1381 aaaatcatcg gaatgtggtt cgcggggatt gaaaagagtc agttggaccg taagacatat
     1441 caagtgaatt tgaagatcgc tctcacgatt ctcggggttt tgattgcttg tatcatagtg
     1501 gcttttttcc tatccaaaca tatcttaaaa gcgattccct cattactggc ggtgttcgat
     1561 cgggctgcgc gcggagatct taccgtggca gcaaagattg ttaccagaga tgagatgcgg
     1621 acactctccg aggggttcaa tcagatgatc cggcagcaac gggagatgat gatcaaggtc
     1681 ttgaactccg cagtacaggt aaaggcggca gcaagtcaaa ttgcagcggt caatcaggat
     1741 ttatcccaac gtacccagga acaggcggcc accctccaag aggttgccgc cacagtggaa
     1801 caggtaaatt cactggcgca tcatacatcc gccaattcca gacaggccga tgaactgtcc
     1861 cggctaatgt atcaggcagc tcaaggcggt ggtcagtcgg tggctgaagc acttcaggcc
     1921 atggacggaa tctccggcag tagccaaaag atatcggata ttattaaagt agttaatgaa
     1981 ctggcgttcc aaacaaatct attggcattg aacgccgcag tggaagcggc ccgcgccgga
     2041 gaacaaggca gggggtttgc ggtagtcgcg gctgaagtcc gaaacctggc cggacggaca
     2101 gcggaagcgg cacgggagat tgagcgttta atccaaggca acggggaaca ggttgagcaa
     2161 ggtaaccagt tggtgaggca atccgccgcg gctctacagg aaatcattaa acagattgag
     2221 ggaacatcca aggtcatcgc cgaagtggct tcagccatgc aagaacaggc tactgctact
     2281 acccagattc aggattcgct agcccaattg aatcaagtta cacaacaaaa tgcagccatt
     2341 gtcgaggaaa ccgcagcatc cagtgagact ttgaatgcgg aagccgaggt attacacgaa
     2401 atggtaagcg gatttaaggt ggagtccgag gttgatgcgg cgagtaaggt ccaatccgaa
     2461 acgaacccgc tacgatcaag ttcatcgagg ttagctccga tgggcgatac tccgagtaag
     2521 cagactcctg ccatggcaat tccgttgacc aacccgcaat ccccggaaac gcagatcccg
     2581 caggagtcca ttttgcagac gctgccccgc cggagacgtc cggaaccccg gccgttaagt
     2641 ggtaagcctg aaccatccga ggatggctgg gagaagtttt aatcgatagg cggagtgaaa
     2701 agccctttgc ctaaacttag tgaatgaatc catgttccct actgttttca atgacgatcc
     2761 gggcggcttc cgccggatgg tcggctattc ttaacagatc cagatcgtgg cggccgatgc
     2821 aaccagcggg aagcatttga tcactcatcc agtcgcagag gcctttccaa tatgaactgg
     2881 cgtaaagaat gatgggaaag tgatcgatct tgtcggtttg gacgagggtg agcgcttcaa
     2941 agagctcatc catggttcca aaaccgccgg gcataatgat aaaggcaatt gcgtatttga
     3001 caaacatcaa tttacgaacg aaaaaataac ggaatttcag ggcgatggtt tgataggagt
     3061 tgggttgttg ctcctccgga agttgaattg agcatcccac cgaggttcct ttgacgttaa
     3121 aggcccctaa atttgcggct tccataattc cgggaccgcc tccggaaagg atcgcgatgc
     3181 cggcggcagc cagccgcgtc gctatatcaa tggtagcttg gtaataagga ttgtccggcc
     3241 gcaaccgagc cgaaccgaag atcgctacag ccggaccgat tttggaaagg gcatcgaaac
     3301 cttccaccat ctcaccttgg atccttaaga ttcgccatgg gtcctcggat gtaaaatcgt
     3361 gactgggcgg gcagggactt tgaaacaact ccttatcttc attgtcgtac attcctttcg
     3421 gctgttgcaa ttggatcacc aaaatcacct cgggttatat ggatgatccc catactgggc
     3481 tcgggatacg ttcctggatt tgcctgtagt ttatccccat ttcccttgta tcaaccgttt
     3541 gcctctattg gccctgattc accataaagc ggtttagacg gtatatttcc cggtggtttg
     3601 gcgcagattt acatcgggag gtgaacgaca tggcgaaaca ggaccgtaag gcttcaaccc
     3661 aaaacggtaa agagaagtta aaatgggaaa ccgcaaggga gttggggcta gatgatgatt
     3721 tggctgccgg cggtgatgaa ttatccgtca aggaggccgg aaaaatagga ggccagatgg
     3781 ttaagaagct cattgccgag ggagagaagc agattgtcca ggaggattcc ggggaaggta
     3841 atgcttaggt tgaataggta catattctga aatcaatgga tctttttaac ttgtaatggg
     3901 aaacggccgt caggccgttt tttatgtcct taaataataa aagtatttcc aagtaaaagg
     3961 gatcggcgtt tgtcagtcaa ataactatct atttggtgcg gactccttct ttttgccctt
     4021 atcaaaataa aaccgattcg gagaaattcc atggctgatt ggggagggat tcccatgttc
     4081 ttgccgggac cggccggata tggattcgtc gagaagtagc agcaatttac taacactctg
     4141 tgcactgttt tcgctaagtc cgggcaggat ttatcgcggc ccggccgaat atattatgtt
     4201 gaatcgcacg aataaaaggt tgggcgggag tgattcggtg aggcattttc tattcaaaaa
     4261 ccgtttccca tgggtactct tggtgggatt gctttttttg tttattttta ccccgttttc
     4321 ctggggtaag gagaagtact tcccgctcga caaggtgaaa ccggggatgt ccggatttgg
     4381 ttataccgtt tttggaggca ccaagatcga gaagttcgat atcaaagtat tggccgtctt
     4441 ggacagcagt gccgatcagg gtaagttgat catggtgcgg ctttccggga aacgtctggc
     4501 ggcaaacggc ggactggcgt caggaatgag cggcagtccg gtttatatcg gtagcaaatt
     4561 gatgggtgcc atcagttacg gatttgagaa tgccgatccg tatctggcaa tggtgactcc
     4621 gatcgagacg atgcttaaat tattgccaga tcaggggaat tctgtgagcc tctcgggtat
     4681 caaccgtttg tacctggggc aagttgtaac cgctgtccga aaacattccg gtctggtggg
     4741 gtcatttaaa cccgtaccac tagctactcc ggtaattcta tccggcatgg gtcaacgggg
     4801 ttacgagatg gtacagaggt cgctgcaacc gcttaattta caaacggtct atgcatctaa
     4861 tttcggcgga gtgaaactag cggagggcaa aacgaatctt cgtcccggca gtgccatcgg
     4921 ggtgcagctc atcgccggtg attatcaagc ggcagccatt ggtactgtca ctctctgtga
     4981 tggtccggat ttcttggctt tcggtcatcc tttcaccaat cggggaactg tcgattattt
     5041 ggccaatgag gctttcatct atcagacagt caagagccaa gtactgccgt ttaagctcgg
     5101 ggctccgatc cgggcaatag gccggatctt tcaggaccgg caagccggaa tcgcgggccg
     5161 cttgggtgag ttgcccagct tgattccggt cgttgtcaac gttacagacc gggatcggaa
     5221 gttttcaaac caacgtcgat ttcaggtggt tgccaatgaa caattatatc gcgacctgat
     5281 catctccgga gtcaccgatg ccattgacca gacgatcgac aggatcggca gcggtaccgc
     5341 ccaggtgagg ttggtcattg ccggtgcgga gcgggatcct ctccttacca gggagaacct
     5401 attttatggc aaggacattg cggtgaattg tcttaaggat ctgcgccaag ctttggatct
     5461 gatctcaacc aatgaatttc tggccgttcc aatccgctcg atcgcagtga atgtcgaagt
     5521 atggaataag caagattctg cccgtattgt atcgctagct ctggatacgg atccgtccaa
     5581 actcaaaccg ggaagcagtt ttaagctaat cgcccgatta catacatacc gtggtgagga
     5641 tctgactgta cctttccagg tgaagatacc accggatact atacctggca aactaatcct
     5701 cagtcttagc ggtggctcca aagatcttcc ggatgagaca gaggactcgg agaaggtgcg
     5761 cacggatgaa ggactggatg gttccggccg tatcggatcg ttccaggaac tcctgaataa
     5821 ttattccaat gcaccgctga ataacgagtt ggtcctggat tacgtcctgc cggactccgg
     5881 gaccaagagt gatgcttccg gcaaaatcgg ctccatcccc ccgggactgc aattgaaagt
     5941 tgccagcgac tattttgttt tgggagatgc ccggttgacc ttggaaatat tggcacctta
     6001 agcgtttcgt ccgggtctct cggtaatctg atttcactag gctaaaggta ttggtgggcc
     6061 ccggaccaag tgaatcgggg ccgacaatac aggaattatt tataaagtcg ggtataagca
     6121 attgaaggta gaaaaaagaa cccaacaaaa aatcaactgg gcgatcttcg ggggcgcatg
     6181 cttgtttttg gcgctttttt tcgcacaata ttttttatta agcgatcaag ctctgaccgg
     6241 gaagattcgg aatgcgttac aggcaagttt gcgtggaaac gggttgcagc tccggatcga
     6301 gagggtccat ctaagcgggt tgaatgaatt taccggatcc ggtttgatct tgtccgatgc
     6361 cgggagtggc cgccttttct ttgccgcgga acaggtccgc ctggaattcg atgcgctatc
     6421 cttcctcagc aattatcgcc atccggaggc ttgtattcgc aagatccgaa tcatcaagcc
     6481 ccacctggtt ttggaacgca gaacctccgg atggaatttt gagcgatatt ttcctcaaac
     6541 cgggcaagtc atgcaattta aagccacgct tcagttggag aaggggttgg tggaattcgg
     6601 ggatttccag tacggaaatt atcggctttc tcaagttgca ggaaagatcg acctttcctc
     6661 gtacccggct gtcttttgga atttgaaagg cctttccaat cttggccctg cctcccaatg
     6721 gaccagtaac ggcagtatgc gcctggatca acaaggggga aggggaagct tgacggttcg
     6781 tcagggtttg gccgcccgtg cggcgaaatt tttgcctcgt cctttcgaat atcggattcg
     6841 ttccggcctt gccaacctcc gcttacgatt cggctggaac aggcagaatt tttggattga
     6901 agacggtcgg gccacaattt ttgaagccgg aatcagcttg ccttggtttt cccggccgtt
     6961 tagggtcgag tacttacagg ccgacttctc gcccgcagag ttcaagctgg agaatggccg
     7021 gataaaatac ggtgatacct ccatcaaggt tcggggaaga ttcgatgtca aaaaggctac
     7081 atttgatggt gaagtttccg cttcacggtt ggtgttaaca gatctgatcg gtatagctcc
     7141 aacaataaaa gatttcgcac cgtccggaac cgcgggactc gatttgaggg tcaacggtcc
     7201 gttaagcgca ccggtatgga acggctggtt gacgctaaaa caggcggcgt ttacacctgt
     7261 ggccggagag agagttgccg ggataaatgg ccgtttacgc attcatgaca atgatctggc
     7321 tgtagaaaat ctccagggta cctggcacgc tactgacatc cgtgtgaacg gaacagtacg
     7381 caatctgttg cgtcccgatt tgaatctgac catcgcagcc agcgaatttg atctgaaaaa
     7441 tatcccctgg gccgagaagt ttcctggttt ggaacttaaa accggatccg gcgactggtt
     7501 caccgcgaca attagcggtg ggttgaaccg gccaaccatt accggggcga tcgactttga
     7561 acaagtgact taccaaacca ttccgatcaa tgggtttcag ttccgtttcg ggttggatcc
     7621 ggtgctatcg accctgaaga ttctcgatct gcagggctcg ttcggagacg ggcaactgaa
     7681 cgccaagggt gagattcact atgataacac ccacctgcaa tggttgatta ccggttcagc
     7741 ctccggacta tctttgactc aattccccct caagaggccc ttggcattca aagggatcat
     7801 cggggcagag gcgattttcc ggggcgattg gaccttcgga acgccttttg atatgggaaa
     7861 ggtctttggg acattatcaa gccgacaatt ggatctgaat ggggttcgtc ttgacgaggc
     7921 agaaggaatc ttcagttggg atcaaaatgc tttcaccatc gatactgtac aggccaaggc
     7981 cgactccggt cggatcttcg ggtatcttac ctggcgtcag gatacgctgg tagtggatat
     8041 caacgcggag aaccttcgct tgcagaagtt ttttcccttg gggccgaaaa tccctctcga
     8101 tggcctgttc gaaggaagtg ttacatttga aggtcctcca tccgatctaa aaggctggat
     8161 taacggcact tttaccgaag caacctggga ttcacggcca ctcggagata ttagcggccg
     8221 cttggcctat aacgatcaaa agcttcatat cgaggagtta cgggctgcaa ccccgaccgg
     8281 agagttcgtt gctaccggcc aggttgattt gacgggtgat cccatgttac aggttaacct
     8341 gaccagtgaa gcttgtgatc tgagtggtgt catgaaatgg ctgccgctag acccggcgtt
     8401 gaaaccggaa ggcatagccc gaattaacct ggatttgaaa ggggcgctcg cgaatccggg
     8461 attacatgga gtaatccaac tgactaaccc cggtctgatg aatatccgat tgaatcgtgg
     8521 agagattgag ttcgaaggcg atttgagacg gatcacgctt gcccggtttg aactccagcg
     8581 ggaccgggcg gtcctacaat taaaagggga catcagcctg gaacaatatg a
//
LOCUS       DASVMB010000345         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_265620,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000345 DASVMB010000000
VERSION     DASVMB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265620"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF531_09825"
     CDS             complement(<1..306)
                     /locus_tag="VF531_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7714310.1"
                     /translation="MNIGQERQMQLTDKKSGELFENKNTPIIDKVDVVVAGGGVAGFT
                     AAIAASRNGAKVILVEQAGYLGGYITGGLVNYMTPYKDWYEQIVGGLAEEIVEKMRQI
                     "
     gene            complement(361..2055)
                     /locus_tag="VF531_09830"
     CDS             complement(361..2055)
                     /locus_tag="VF531_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7714311.1"
                     /translation="MIFLQAAEVVVRILENEGIQTAFGIPGAAINPVYKYLKDSRIKH
                     YIARHEEGAIHAADGYFRASGRMALAICTSGPGATNFVTGLYTAQIDSIPLIAITGQA
                     DSNWFGKEPFQCVDIATIAKPVAKATWCVTNPADIPEVMRQAFHTARSGRPGPVLIDL
                     PLNVQTAEIDFNPEGYKPLVVAKPGPDLEQVAGAMDLIMKSKNPVMILGGGVIISGAA
                     AEFKALAEYLSIPVIMTYMGKGGLAPDHPLNAGHAGIQVGQPIGNEVFLGSDLVIGVG
                     CRFSDRHTGKLDTYVKGRKFIHIDIEPSQIGRVVPAEVGIVSDAKLALVALLDEAKKR
                     GYHPAPGDRVKALPERRKQLARKTDYDNSPIKPQRVYEEINKFFPENTMFTTGCGLTQ
                     IWSGQLQEIKLPRRYLPSGGAGTLGYELPAAVGAKIACPKDPAVVVVGDAGLLFMIEE
                     LGMAGQHNVPVIVIVVNNGYLSLIRQNQKYAYQFEHGVSLWYNEDLSKAQVYPDNVKI
                     AEGFGAKAERVMKMEDLPKAFARALNSKVPYLIDVVVERETDCSMGGSIDGVKEFV"
BASE COUNT          524 a          602 c          465 g          584 t
ORIGIN      
        1 aatttgccgc attttctcaa caatttcttc cgctaagccc ccgacaatct gttcgtacca
       61 atctttatag ggtgtcatgt aatttaccaa tccgccagta atatacccgc cgagatatcc
      121 cgcttgttcg actaaaataa cctttgcccc attacgagaa gcagctatgg cggccgtaaa
      181 tccagccacg ccgccaccag ccactaccac atccactttg tcaattattg gtgtattctt
      241 gttttcaaat aattccccgg attttttatc tgttagttgc atctgccttt cttgaccaat
      301 attcattgtt ctccctctca tccagttttt tagatttaag tatattgaat cgaaactctt
      361 ctacacaaac tccttcaccc catcgatgct tccgcccatg gagcaatcgg tctcccgttc
      421 cacaacaaca tcgatcaggt aagggacttt tgaattgagc gcccgcgcaa aagccttcgg
      481 caagtcttcc atcttcatga ccctttccgc cttagcaccg aaaccttcgg cgattttgac
      541 gttatccggg taaacctgag cctttgagag atcctcgtta taccacaggc ttacaccatg
      601 ctcgaattgg taagcgtatt tctgattctg gcgaatcaaa cttaaataac cattgttgac
      661 cacgatgaca atgacgggca cattatgctg accggccatg cctaattctt caatcataaa
      721 gagtaatccg gcgtctccaa ccacaactac ggccggatct ttggggcagg cgatcttggc
      781 cccgacggca gccggcagtt cgtaaccaag ggtaccggca ccgcccgacg gcaaatacct
      841 ccttggcagc ttaatctcct gaagttgtcc cgaccatatc tgcgtcaggc cgcaaccggt
      901 ggtgaacatc gtgttctcag ggaagaattt gttgatctct tcgtaaacgc gttgcggctt
      961 aatgggagag ttatcgtaat ccgttttccg ggccagttgt ttacgacgtt caggcaacgc
     1021 cttgaccctg tcgccgggag cgggatgata tccccttttt ttcgcctcgt ccagcagtgc
     1081 gaccaaggcg agcttggcat cggatacgat cccgacttcg gcaggtacaa ctcttccgat
     1141 ttgagaaggc tcaatatcaa tgtgaatgaa tttccggcct ttgacatatg tatcaagttt
     1201 cccggtatgc ctgtcgctga aacggcagcc aacaccgatc accaggtccg aacccaagaa
     1261 aacctcattc ccgatcggtt gcccaacctg aataccagca tgacccgcat ttaacggatg
     1321 atccggagcc aagccgcctt ttcccatata tgtcatgatt accggaatcg ataagtactc
     1381 agccagtgct ttgaactccg cggcggctcc ggagatgatc actccgcccc ccaaaatcat
     1441 caccggattt ttacttttca tgatcagatc cattgccccg gccacctgtt ccaaatcggg
     1501 tccgggtttg gccacgacca gcggtttgta tccctcgggg ttaaaatcaa tttccgctgt
     1561 ttggacattc aaaggaaggt caatcaacac cggtcccggt cgtccggaac gggcggtgtg
     1621 aaaagcctgc ctcataacct ccggtatatc ggcgggattg gtcacgcacc aagtcgcctt
     1681 ggcaaccggt ttggcaattg tggcaatgtc cacgcactgg aacggctctt taccaaacca
     1741 gtttgaatcc gcttggccag tgatggcgat caatggaatg gaatcgattt gggccgtata
     1801 caaaccggtt acaaaattcg tcgctcccgg tccggaggtg cagatcgcca aagccatcct
     1861 gcccgaagcg cggaaataac catccgccgc atggattgcc ccttcttcat gccgggcgat
     1921 gtaatgcttg atacgggaat cctttaaata tttgtatacc gggttaatgg ctgctcccgg
     1981 tatgccgaaa gccgtttgaa tcccttcatt ttcaaggatt ctgactacta cttccgctgc
     2041 ctgcaaaaat atcactcctt gttaaaatga tatttactat ttcgtcacat gcctaagatg
     2101 accttctgta ttataaaata gtccatattg tggactaatg ttttcacatt gtagacttta
     2161 ataaaatgtg agaat
//
LOCUS       DASVMB010000346         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_707446,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000346 DASVMB010000000
VERSION     DASVMB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707446"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1608
                     /gene="xseA"
                     /locus_tag="VF531_09835"
     CDS             406..1608
                     /gene="xseA"
                     /locus_tag="VF531_09835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7714312.1"
                     /translation="MEPKYISVSQLTYALKRLIEENFPLDGVWIQGEISNFTAHSSGH
                     LYFTLKDETACLKCVLFKSQARGLSFRPGDGMAVSVRGRIAVYEKAGQYQLYVDTMEP
                     AGMGDLYQRFECLKARLAAEGLFDPGRKRPLPSLPQRVGLITSPTGAAVQDMIKILRR
                     RRPNLDILLIPARVQGEDAPASLVKALDEAARFEALDLVIIGRGGGSLEELWAFNDER
                     VARAIADFPKPIISAVGHETDFTIADFVADLRAPTPSAAAELAVPDQTAMERHIVSMA
                     DRLKQAMSRGIQSKRRDILNLLRTKALRFPKYGLDLRRQELDLISERMSRRIRQDLLI
                     KRERYCKTLGKLDTLSPLATFKRGYSLAQKPDGSFIRRSDQVEIGERLRLTLAVGKID
                     CQVLDKEV"
     gene            1610..1870
                     /gene="xseB"
                     /locus_tag="VF531_09840"
     CDS             1610..1870
                     /gene="xseB"
                     /locus_tag="VF531_09840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7714313.1"
                     /translation="MENPNDNQASERSYEEALKRLEEIVERLEGGDLTLEESLSLFEE
                     GIGLAKFCNGKLDAAEGRVEMLLGFEGNQPQTGPFKLSGEEE"
     gene            1878..2969
                     /locus_tag="VF531_09845"
     CDS             1878..2969
                     /locus_tag="VF531_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7714314.1"
                     /translation="MESHISKAQFIQELKSREAGIVEIFNQPRYRQSFRPAELEQAVY
                     SYLVRPAKRFRPGLLLFSCGAVGGREEQAVYAAAAVEVYHTWTLVHDDVIDNDPLRRG
                     SPTVHEQFRRRGLELGYSSGDAEALGRNMAILAGDLQHAWSVRLLLDSAELGVPLLVL
                     IDLAHRLEVEVTNELLAGEALDVLFAKRPIGELQEAEILEMLELKTGVLYQFAGMAGA
                     AIGLAQPPTGNELIQGIGRFTRRCGTAFQLQDDILGITGNEQVLGKAVGSDIREGKRT
                     LIVLHAYHEADITDREFLETWLGNPAAGPAEIGRINRILIDNGGIEYTRTLAAEIVAA
                     AVDELKPLTDSHYKELLLTWARYVVERDF"
     gene            3007..>3184
                     /locus_tag="VF531_09850"
     CDS             3007..>3184
                     /locus_tag="VF531_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX7714315.1"
                     /translation="MQTFLREITYNHVIWTSLIAWFIAQLLKGIFYFAKEKKINFRRF
                     VGAGGMPSSHSALVA"
BASE COUNT          758 a          741 c          909 g          776 t
ORIGIN      
        1 atgatgacaa aagaaacgga gtttggtaag gatttttgta gctgtcccag ccaaaggtca
       61 gattataagt gaaaacaggc aaccgattcc cttacccggc aggattttta ggcgatttat
      121 ggaatacaac ataaaggcaa agagtcgtga ggcacgaggc atcgcgtcct gcgggacgcg
      181 cggcaactca ccccgagata ctcatacgct tccagtatga cactattccg cgttttaaaa
      241 cgcgcactct ctttttcgaa agagaggggt cggggtgagt tgcccttaga cttatgaata
      301 tagactctcc gactttcgac tagaataaat cgcttacaat tctcatgcct cgcgcctcat
      361 gccttaatcc tctttagaac tgcgctttca agaaaggatt tatctttgga acccaagtat
      421 atttcggttt cacaactgac atacgccctc aagcgattga tcgaggagaa ctttccactc
      481 gacggggttt ggattcaggg agagatctcc aattttactg cccattcatc ggggcatctt
      541 tattttacat tgaaagatga gaccgcttgt ttaaaatgcg ttctgttcaa gtcacaggcc
      601 cgtgggttat cctttcggcc cggggacgga atggccgtat ctgtgcgggg gcgaattgca
      661 gtctacgaaa aagccggtca atatcaactt tatgttgata ccatggaacc tgcgggtatg
      721 ggcgatctct atcaaagatt cgaatgtttg aaagcccgac ttgcagccga agggctattc
      781 gatcccggcc gcaagcggcc gctcccctca ttgccccaaa gggtcggatt gatcacctca
      841 cccaccgggg ctgcggttca ggatatgatt aagattttga gacgccgccg gcctaatctc
      901 gatattttat tgattcccgc ccgggttcag ggggaagacg ctccggccag tctggttaag
      961 gcactggatg aagcagcccg gtttgaagcg ttggacttgg tgatcatcgg gcgaggcggc
     1021 ggttcccttg aagaattatg ggcttttaat gatgaaaggg tagcccgggc tatcgccgat
     1081 tttccaaagc caatcatctc cgcggtaggc catgagacgg attttactat tgccgatttt
     1141 gtggcggatc tccgggcgcc aacgccctcg gctgcggccg agttggcggt accggatcaa
     1201 accgccatgg aacggcatat cgtctctatg gctgaccggc tcaaacaggc aatgagccgg
     1261 ggaatccagt cgaaacggcg ggatattctt aatttgttac ggaccaaagc cctacgtttt
     1321 cccaaatacg ggctcgatct gcgccgtcag gagctggatt taatctccga aaggatgtca
     1381 cgccgtatcc gtcaggattt actgattaaa cgggaacgat attgcaaaac tttgggcaaa
     1441 ttagatacat tgagcccttt ggcgacattt aaacggggtt acagcctggc ccaaaaaccg
     1501 gacggcagct tcatccggcg tagtgatcaa gttgaaatcg gggaacggct ccgattgacc
     1561 ctagctgttg gaaagatcga ctgccaggtg ctggataagg aggtctaaga tggagaaccc
     1621 aaacgacaat caagcgagtg aacgcagcta tgaagaggcc ttgaaacggc tggaggagat
     1681 cgttgaacgc ctggagggcg gagatttaac cctggaggag agtctgtcac tgtttgagga
     1741 gggtatcggc ctggccaagt tttgtaacgg caaattggac gcagccgaag gccgggtcga
     1801 gatgttgctc ggctttgagg gtaatcagcc ccaaaccggc ccgtttaaat tgagcgggga
     1861 ggaggaatag ccgactgatg gagtctcata taagtaaggc tcagtttatt caggaactaa
     1921 aaagccgcga ggccggaatt gtcgagatct tcaaccaacc ccgctatcgc cagagttttc
     1981 gccctgccga actcgaacag gcggtttatt catatcttgt cagaccggcc aaacgttttc
     2041 ggcccgggct gctccttttc agttgcgggg cggtaggagg acgggaggaa caggcggttt
     2101 atgccgcggc tgcagtagaa gtataccata catggacgtt ggttcatgat gatgtgatcg
     2161 ataacgatcc gctacgccgg ggttcgccga cagtccatga acagtttcgg cgccgtggtt
     2221 tggaactggg gtactcttcc ggggatgccg aggcattggg aaggaatatg gctatcctcg
     2281 ccggtgatct gcagcatgct tggtcggttc gactgctgct agactcggcc gaattgggtg
     2341 tacctctgct ggtcttgatt gatctggcgc accgcttgga ggttgaggtg accaacgaat
     2401 tgttggccgg agaagctttg gatgttctat ttgccaagcg tcccatcgga gagttacaag
     2461 aggcggaaat cctcgaaatg ctggaattaa aaaccggggt actataccaa tttgccggga
     2521 tggccggcgc cgccatcgga ctcgctcaac ctcccacggg gaatgaactc attcaaggta
     2581 tcggccggtt cacccggcgt tgcggcacag cctttcagct tcaggatgat attttaggga
     2641 tcaccggtaa tgaacaggtg ttgggtaaag ctgtgggatc ggatatccgg gagggtaaac
     2701 ggactcttat cgtcctgcat gcttaccatg aggcggatat cacagatcgg gagtttcttg
     2761 aaacgtggct gggtaacccg gcggcgggcc cggcggagat cggcaggata aaccggattc
     2821 tgatcgataa cggcggaatc gaatataccc ggacgcttgc ggcagagatt gtcgcggcag
     2881 ccgtcgatga gcttaagccg ttaacggatt cgcattataa ggagttatta ttgacctggg
     2941 cgcggtatgt ggtggaacgg gatttctaga gatcaaacat cagggtctta gaaaggggaa
     3001 tcatttttgc agacgtttct gcgggagata acatataatc atgtcatctg gacttctttg
     3061 attgcctggt ttattgcaca gcttcttaaa ggcatttttt atttcgccaa ggagaagaag
     3121 attaatttcc gaaggttcgt cggtgcaggc ggtatgccca gttcccattc ggctttggta
     3181 gctt
//
LOCUS       DASVMB010000347         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_43198,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000347 DASVMB010000000
VERSION     DASVMB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43198"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..773)
                     /gene="rpsD"
                     /locus_tag="VF531_09855"
     CDS             complement(147..773)
                     /gene="rpsD"
                     /locus_tag="VF531_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7714316.1"
                     /translation="MARYNEAVCRLCRREGEKLYLKGERCYTQKCSVGKRSYAPGQHG
                     QDRKKISEYGIQLREKQKLRRIYGVLERQFSGYFSMAERKQGITGQNLLQILETRLDN
                     VVYRLGFGASRKEARQLVLHGHFRVNGKKVTIPSYLLKVGDAVSVKEGSLESTKIKAN
                     LESAASHTIPEWLELDLNNHRGTVKAIPTREQIDIPVQEHLIVELYSR"
     gene            complement(804..1217)
                     /gene="rpsK"
                     /locus_tag="VF531_09860"
     CDS             complement(804..1217)
                     /gene="rpsK"
                     /locus_tag="VF531_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7714317.1"
                     /translation="MPAANTKPVAKKGVRTRKKERKHVDSGVAHIHSTFNNTVVTITD
                     TAGNVLSWSSAGHMGFKGSRKSTPFAAGMAAEEAAKIAMDYGMKNIEVYVKGPGSGRE
                     AAIRSLQAAGLFVNMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(1232..1603)
                     /gene="rpsM"
                     /locus_tag="VF531_09865"
     CDS             complement(1232..1603)
                     /gene="rpsM"
                     /locus_tag="VF531_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7714318.1"
                     /translation="MARIAGVDLPRDKRVEVALTYIYGLGRTTANEVLSKTGVNPDTR
                     VRDLTEEEVTKLREVIDHDYKVEGDLRRDESMNVKRLIEIGSYRGLRHRRGLPVRGQR
                     TKTNARTRKGPRKTVGAKRKA"
     gene            complement(1681..1794)
                     /gene="rpmJ"
                     /locus_tag="VF531_09870"
     CDS             complement(1681..1794)
                     /gene="rpmJ"
                     /locus_tag="VF531_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX7714319.1"
                     /translation="MKVRPSVKPVCEHCKIIRRKGHVLVICDNPKHKQKQG"
     gene            complement(1906..2124)
                     /gene="infA"
                     /locus_tag="VF531_09875"
     CDS             complement(1906..2124)
                     /gene="infA"
                     /locus_tag="VF531_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7714320.1"
                     /translation="MGKQDVIEVEGTVVEPLPNAMFRVELENGHKVLAHISGKMRMNF
                     IKILPGDRVTVELSAYDLTRGRIVYRYK"
     gene            complement(2117..2437)
                     /locus_tag="VF531_09880"
     CDS             complement(2117..2437)
                     /locus_tag="VF531_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HEX7714321.1"
                     /translation="MTVMFCPTVDGLRRGQMVRSTQGRDFDQYYLIVDLVGDRYIMVV
                     DGSYHRLARPKKKNLKHVKITMLVAKNVEKALMNKESLTDSVIVAAINKLKNELEEGE
                     RLNG"
     gene            complement(2437..3210)
                     /gene="map"
                     /locus_tag="VF531_09885"
     CDS             complement(2437..3210)
                     /gene="map"
                     /locus_tag="VF531_09885"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7714322.1"
                     /translation="MIVLKSASEIEIMRISGSITAAVLEDLRQMIRPGVSLADLDRQA
                     ESLTRHYHAIPAFKGYNGFPASICASVNDEVVHGIPVNRILKEGDIVGLDFGVVYHDF
                     YGDSAITVPVGQISKESQELLRVTEEALYKGIAKAQAGNRLFDISEAVQRHVESNGFS
                     VVRDFVGHGIGRRMHEAPQVPNYVGNDFNPRLKAGMTIAIEPMVNIGSYEVDVDSGNN
                     WTVRTIDHKNSAHFEHTVVITNGEPEILTLLRPELLPRG"
     gene            complement(3207..3860)
                     /locus_tag="VF531_09890"
     CDS             complement(3207..3860)
                     /locus_tag="VF531_09890"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7714323.1"
                     /translation="MNLILLGPPGAGKGTQAELLVKQYHIPHISTGDIFRAAIKEGTS
                     LGLEAKRFMDSGQLVPDEIVIGIVKERLVKPDCESGFLLDGFPRTVPQADALDQFLKG
                     LNRRITAVINIEVDNEILSSRLTGRRVCRNCAAVYHIATKPSKVQGVCDHCGGEVYQR
                     DDDTPATVSNRLEVYTKQTEPLIAYYRSKGLLESFDGTKQIPEIFAAICKVLEAKQK"
BASE COUNT          947 a         1050 c          843 g         1075 t
ORIGIN      
        1 aataatgggg ggagcaaatt ttcagcttcc aggttgattg gtctatcgga tctgagctgg
       61 cgaataactt ccctaaacca aattcaaaca aagcatatgc gtattggttc cgtgttcccg
      121 gttgcaagca attgcaaaac cgtggtttaa cgggaataca actctacgat caagtgctct
      181 tgaaccggta tatcgatctg ctccctggtc ggtatagctt ttactgtgcc acgatgatta
      241 ttcagatcga gttccaacca ctccggaatc gtatggctgg cggcagactc aagattcgcc
      301 ttaatcttcg tcgattcgag actaccttct ttgacggaaa cggcatcccc gacctttaat
      361 aaataggacg gaatggtgac ctttttgccg tttacacgga aatgtccgtg aagcactaac
      421 tgccgggctt ctttccggga agccccgaac ccaagccggt aaactacatt atcaagccgg
      481 gtttccaaga tttgcagtaa gttctgaccg gtaatacctt gtttccgttc ggccattgaa
      541 aaatatcccg aaaactggcg ttccagtacc ccgtaaatcc ggcgaagttt ttgtttttcc
      601 cgaagttgga ttccatattc ggagattttt ttccggtcct gaccatgttg tcccggagca
      661 taactccgtt tgccaacgct gcatttttga gtatagcatc gttcgccctt taaataaagt
      721 ttctcgccct cacggcggca taatctgcaa acagcttcgt tatatcgtgc cataagtttg
      781 tttacacctc ccagaaatat gcttcaaacg cgtctccgct tcggcggacg gcaaccgtta
      841 tgcggaatcg gtgttacatc tttaatcata ttgacaaaaa gtcctgcggc ttgtaacgaa
      901 cggatcgctg cttcccggcc ggatcccggt cccttaacgt atacctcgat attcttcatg
      961 ccgtaatcca tagcgatttt ggcggcttcc tcggcagcca tacccgccgc gaacggggtg
     1021 ctcttccgtg aacccttgaa ccccatatgc cccgcgcttg accaggataa aacgttacct
     1081 gccgtgtccg tgatggtgac cacggtattg ttaaacgtag aatgaatatg cgctacgccg
     1141 ctatcaacgt gtttccgctc ttttttacgg gtgcggactc cttttttggc aacaggtttt
     1201 gtatttgctg ctggcatcgt ttattacctc cttaagcctt tctctttgcg ccaacggtct
     1261 tccgcgggcc tttacgggtg cgggcgttgg ttttggttcg ctggcccctg accggtaaac
     1321 cgcggcggtg tctcaatcca cggtaactac caatctcgat tagccgtttt acattcatag
     1381 actcatcgcg gcggagatcg ccctcgactt tataatcatg atcgataact tctcttaatt
     1441 tggtaacctc ttcctcggtt aggtctcgaa cccgggtatc ggggttgact ccggtcttgc
     1501 ttaaaacctc atttgcggta gtacgcccca atccatagat atatgtcagg gcaacttcta
     1561 cccgtttatc acgtggcaaa tctactccgg caatacgcgc catttgtttg acctcctttt
     1621 aaatcaggcg gggttcaagg ccctatccgt ggcacctcta tcccgcaacc tgtatttcct
     1681 ttaaccttgt ttctgtttgt gtttaggatt gtcacaaatt accagaacgt ggcctttgcg
     1741 ccgaatgatc ttgcaatgtt cgcatactgg tttgacggaa ggtcttacct tcatttggaa
     1801 tcctcctctt gctaatttta ccataaaccg tccaatttat aaatttatca gaaacttatc
     1861 ggggaattta tcgccggttt ccaagacggt cgaggccgtt caaagtcatt tatagcgata
     1921 aacaattcgg ccgcgggtta aatcataggc tgaaagttct acagtaaccc gatcacccgg
     1981 caagatcttg atgaaattca tccgcatctt tccggagata tgggccagaa ccttgtgacc
     2041 gttctccaat tcaacacgaa acatggcgtt aggtaacggt tcgaccacgg ttccttcaac
     2101 ctcgataaca tcctgtttac ccattcaacc gctccccttc ctcaagttca tttttcagtt
     2161 tattgatggc ggcgacaatc accgaatcag tcaggctttc cttgttcatt aacgcctttt
     2221 ccacattttt cgccaccagc attgtaatct taacgtgttt caaattcttc tttttgggtc
     2281 tggccaaccg gtgataagag ccgtccacca ccataatata tcggtcaccc accagatcaa
     2341 cgatcaagta gtattgatcg aaatctctgc cttgagtgga acggaccatt tgaccgcggc
     2401 gcaaaccatc gactgtagga cagaacatta ctgtcatcaa cccctcggca gtaactccgg
     2461 cctgagtaaa gtcaggatct cgggctcccc atttgtgata accactgtat gttcaaagtg
     2521 ggccgaattt ttgtggtcga tagtacggac cgtccagttg tttcccgaat ccacatccac
     2581 ttcatacgaa ccgatattca ccataggctc gatggcgatg gtcataccgg cctttaaacg
     2641 ggggttgaaa tcattcccca cataattcgg gacttggggc gcctcatgca tccggcggcc
     2701 tattccatga cccacaaaat cgcggaccac cgaaaaacca ttggactcga catggcgttg
     2761 aaccgcctcg gagatgtcaa ataaccggtt accggcctgt gctttggcaa tacccttata
     2821 taaagcctct tcggtaacac ggagcaattc ttgagattcc tttgaaattt gccctaccgg
     2881 aaccgtaatc gcggaatcac cgtaaaaatc gtgataaacc acaccaaagt ccaatcctac
     2941 aatatcgccc tctttcagga ttcggttgac cggaattcca tgaaccactt cgtcattgac
     3001 cgaagcacag attgaggcgg gaaaaccatt atagccctta aaagcgggta tcgcatgata
     3061 atgtcgggtc aatgattcgg cctgtcggtc cagatcggca agggaaaccc cgggccgaat
     3121 catctgtctc aaatcctcca aaacagcagc ggtaatgctc ccggagatcc gcataatctc
     3181 aatctccgaa gcggatttta aaacaatcat ttctgttttg cctctaatac cttacaaatc
     3241 gcagcgaaga tttcgggaat ctgttttgtg ccgtcaaagc tttcgagtaa ccctttggaa
     3301 cgataatatg cgatcaaagg ctcggtttgc ttcgtataga cttccaaccg gttactaacc
     3361 gtagccgggg tatcgtcatc ccgttggtag acctctccgc cgcaatgatc gcaaaccccc
     3421 tgaaccttag agggtttagt ggcgatgtga tagactgcgg cgcaattccg gcagactctg
     3481 cgaccggtta aacgggatga tagaatttca ttatccacct caatgttaat caccgcagta
     3541 atccgacgat taagcccctt caaaaattga tccagcgcat cggcttgagg cacagtccgc
     3601 gggaaaccgt ccaataaaaa cccggattca caatcgggtt ttaccagccg ttcctttacg
     3661 ataccgatca cgatctcatc cggaaccagt tggccgctgt ccatgaaacg tttggcctca
     3721 aggcccagag atgttccttc tttgatggcg gcacggaaaa tatcccctgt ggaaatatgg
     3781 ggtatatgat attgctttac cagtaattcc gcctgcgtgc ccttacccgc tccgggaggt
     3841 cccaatagaa tcagattcac gtttgcctcc tatttaccaa aacgccttgt ttaaaaccac
     3901 tgacccccac tagaa
//
LOCUS       DASVMB010000348         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_558580,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000348 DASVMB010000000
VERSION     DASVMB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558580"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF531_09895"
     CDS             complement(<1..551)
                     /locus_tag="VF531_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714324.1"
                     /translation="MKSIRVFISLLVISGLCISYAATTSLTGRLVVVGNVPFTQLVCK
                     IDDHQAYVLKGELLNELTTLQGTTVKLTGDATLLPREGNLPVFTVTDYEILAVGSPNH
                     FVKPFVGILKSQKNKIFLELSSHQQYQINGPMLAKLKKQSGAKVWLTGELKKASWFSS
                     QMILVVDAYQIIRPVSNKKTLITP"
     gene            complement(570..2417)
                     /locus_tag="VF531_09900"
     CDS             complement(570..2417)
                     /locus_tag="VF531_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7714325.1"
                     /translation="MKKYSLAFLCVAFLLVSVLSGCGGGGGTPRASVDGSVVDFFNQP
                     VSDVTVEIAGQATTSTGSNGQFAFSGIPYGQYVMRLSKSGYVSQAFYVLIDRSGYFNP
                     FTIFQTTDNNQGYGQTTGVTQGIYDINASPYQGERTIDATNVDSIVLIPYNLSEYAGT
                     RTVDVTLKFDGQSVNDLNFTAPAKPFKSAQTPGNEILSKLHRDPKGSSWQTIHQMDRN
                     LLLKGLRPSFRSSGQTVQASEIPEGQFYKLFGDNPGYPFTADRVYTGEHCYVYLDRTQ
                     NIPKDKLDQMGRAFDYFYQTMIDTFGDVSAYSIDTDSHVYLVFTELNHDMGGYFWSVN
                     EYTNEQLKNDGYPECSNEKEIVFLQTYGYDDDPTGWFNWEQSATAHEFQHLINYINRI
                     FVANHSESAIDSETWINEGLSQVAQDIAMAGSGQHDPELEPMVAAFLNNISSNSLCTW
                     ATSDSVDPMDYPPAYLFMRYYADRFGEGELKNVEKSSNTGIDSLLNSSITTTNPGLNF
                     ENLFRDWLAAMVLDYLNYSNDSMHYQSWVFSDTDFQNQLGVLMKVGSISIPDTTGIYI
                     YRTDLANVNSIYVSKEGYCGLRILLLPEFGVNFSYRSGSQPEIIRIIPR"
     gene            complement(2594..2770)
                     /locus_tag="VF531_09905"
     CDS             complement(2594..2770)
                     /locus_tag="VF531_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7714326.1"
                     /translation="MVKLHRESCPANHPCPAVRLCPAGAISQNGMSAPTVDATKCIDC
                     GKCARFCAYGVFER"
     gene            complement(3038..3355)
                     /gene="trxA"
                     /locus_tag="VF531_09910"
     CDS             complement(3038..3355)
                     /gene="trxA"
                     /locus_tag="VF531_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7714327.1"
                     /translation="MVNLTEQNFESELLQTDGVALVDFWAPWCGPCRMMGPVLEEIAR
                     DYAGRLNVAKVNVDENPRLAGEFGVMSIPTLIIFKDGQPVDQMVGAMPKAVLEGKLGR
                     WVG"
     gene            3580..3858
                     /locus_tag="VF531_09915"
     CDS             3580..3858
                     /locus_tag="VF531_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714328.1"
                     /translation="MDNDRFQDLVLGHLANITQELTMIKTDVAGLKSDVAELKTDVAE
                     LKSDVATLKIDVAELRQGFAEQKESIKAMADMYGQHELELAKLRRRPV"
     gene            3966..4301
                     /locus_tag="VF531_09920"
     CDS             3966..4301
                     /locus_tag="VF531_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714329.1"
                     /translation="MMDNERFQDLVLSHLANITQELTMSKADITELKSDVAGLKTDVA
                     TLQANYIELKAGQDTIMTRVDKLAEELTELRQEVRQGFAELKESIKAMADMYGQHELE
                     LAKLRRRPV"
BASE COUNT         1245 a         1012 c          910 g         1190 t
ORIGIN      
        1 ggagtgatga gtgttttttt gtttgaaacc ggacgaataa tctggtaggc atcgactact
       61 aagatcatct gactgctgaa ccatgaagct ttttttagtt caccggtcaa ccaaactttg
      121 gctccggatt gtttttttaa ttttgcaagc attggaccgt taatttggta ttgttggtgt
      181 gaactcaatt ccaggaaaat cttattcttt tggcttttta aaattcctac aaacggttta
      241 acgaaatgat tgggactacc caccgctaga atttcataat cagttactgt gaaaacaggt
      301 agattccctt ctctgggaag taaggttgcg tctccggtca gcttaacagt agttccctgc
      361 agggttgtta attcgttaag caactcccct tttaaaacat aggcttgatg gtcatcaatt
      421 ttacaaacca actgagtaaa cggcacattc ccgactacta ccagtcttcc tgttagactg
      481 gtagtagcgg cataacttat gcataatccg gagatgacca acaaagaaat gaatactctt
      541 atactcttca tcaaaattct cacccctgct taccttggaa tgatcctgat tatttcgggt
      601 tgtgagccgg atctatacga gaaattaacc ccaaattctg gaagcaagag gatcctcaga
      661 ccgcaataac cttcttttga aacgtaaatg gaattgacat ttgctagatc cgttcgataa
      721 atatagattc cagtggtatc ggggattgaa attgaaccta ctttcattaa aactcctaat
      781 tgattttgaa aatccgtgtc gctgaaaacc caagactgat aatgcatact gtcgttgctg
      841 taatttaagt aatccaaaac catcgccgcg agccaatccc ggaataggtt ttcaaaattc
      901 aacccggggt tggtggtagt aatacttgaa ttaagtaaac tatcgatccc agtattcgaa
      961 cttttttcaa cattctttag ttcgccctca ccaaaccggt cggcgtaata acgcataaac
     1021 aagtatgccg gcggataatc cataggatct accgaatcgg aagtagccca ggtgcataag
     1081 gagttgctgg agatattatt taaaaatgcc gcaaccattg gctccaattc tggatcgtgt
     1141 tgcccggagc ctgccatggc aatatcctga gctacttggg acaaaccttc gttaatccag
     1201 gtttccgaat cgattgccga ctcgctatgg tttgccacaa aaatccggtt gatataattg
     1261 atgagatgtt gaaattcatg ggcggtcgcg gattgttccc agttgaacca accggttgga
     1321 tcatcatcat acccgtaagt ctgaagaaac acaatctcct tttcattgga gcattccgga
     1381 taaccatcat tttttagctg ttcgtttgtg tactcgttta ccgaccaaaa atacccgccc
     1441 atatcatggt tcaattcagt aaagaccaaa tagacatgag agtcggtatc aatactatag
     1501 gctgatacat ccccaaaagt atcaatcata gtttgataga aataatcaaa ggccctaccc
     1561 atttgatcta atttatcctt aggaatgttt tgggtcctat ccagataaac atagcaatgt
     1621 tctcccgtgt atacacgatc ggcggtaaat ggatatccgg ggttgtctcc gaacaattta
     1681 taaaactgac cttctggaat ttcactggct tgaacggttt ggccactaga cctgaagctt
     1741 ggtcgcaacc ctttcaaaag aagattgcga tccatctgat gaattgtttg ccaactgcta
     1801 cccttcggat ctcggtgaag tttggacaat atttcgtttc cgggtgtttg agccgactta
     1861 aatggtttgg caggagctgt gaaattcaga tcgttcactg attgaccatc aaatttaaga
     1921 gtgacatcta ccgttctggt tcctgcatat tcactgaggt tataaggaat aagaacaata
     1981 gaatctacat ttgttgcatc aatggttcgt tcaccttgat agggtgaagc gttgatatcg
     2041 tagattcctt gggttacacc ggtggtctga ccatatcctt gattgttatc ggtggtctgg
     2101 aatatggtaa acggattgaa ataaccgcta cgatcaatta atacgtagaa ggcttgggaa
     2161 acataaccgg atttcgataa acgcataaca tattggccgt aaggaatccc ggagaaagcg
     2221 aactggccgt tactgccggt actggtagtg gcttgtccgg caatctccac agtgacatcg
     2281 ctaacgggct gattgaaaaa gtctacgact gagccgtcaa cgcttgccct tggcgtccct
     2341 ccgcccccgc cacaaccgct taagactgag accaataaaa aggcaacaca caaaaaagcc
     2401 agactgtact ttttcattcg ataatcaccc tcgataacaa tttgttcttt atcttaatta
     2461 gaaattgcct caattcttaa aatatcacat ttcaaatctc cctgtcaacc aaattctgga
     2521 taataaaaaa ccgtgcctgt ttcaatttta aggcacggtt cattattttt tgataatcaa
     2581 agaaattttt ttgctatctt tcaaataccc catacgcgca gaaccgggcg catttgccgc
     2641 aatcgatgca tttcgtcgca tcgaccgtgg gagcgctcat cccgttttga gatatggccc
     2701 cggcggggca aagccgcacc gccggacagg gatggttggc gggacaggat tcgcgatgca
     2761 atttaaccat tcattcaccc ccgaagagtt agttattcat cattggtttt ggactttcac
     2821 aatccaagta tttaaggccc tcatcccttg ggctatttcg agttgtatgc attcctgcta
     2881 ccgtgtagaa aggaacaagt agtctatgga gtttaggcat tcatttccct cccccatctg
     2941 aatgagcatt aatgataact acttcatggg ggagggccga cccggatctt aactaagggg
     3001 tgagggcatg cctcacgcct catgcctttt cccccaccta ccccacccat ctcccgagtt
     3061 tcccctcaag taccgccttg ggcatggcgc cgaccatctg gtcgacgggt tggccgtctt
     3121 tgaagattat caacgtgggg atggacatga ctccaaactc tcccgccagc cggggatttt
     3181 catccacatt tactttggcc acattcaaac gtcccgcgta atcccgggcg atttcttcga
     3241 gtaccggtcc catcatccgg cacggcccgc accagggcgc ccagaaatcc accaatgcca
     3301 cgccgtccgt ttgcagcaat tcagattcaa aattttgttc cgttagattc accattggaa
     3361 ttcctcctta tcaatatacc ctacagggta tagtatatca aattctacaa aaaagtcaat
     3421 catcctattg ccgcaagcat cacatggaaa tcccggtata tgaatagagg ctttgaaaag
     3481 gaaaacgaaa gatgtttata atattgatga cttatgataa actgtaatta gtacatcaat
     3541 aattccataa tttagaaaat attaaggagg gaaaatgcga tggataacga ccggtttcaa
     3601 gatctggtat tgggacattt ggccaatatt actcaagaat taaccatgat caaaaccgat
     3661 gtcgccggac tcaagtccga tgtcgccgag ctaaaaaccg atgtagccga actcaagtcc
     3721 gatgttgcaa cgcttaagat cgatgttgcc gaactccggc agggcttcgc ggaacaaaag
     3781 gaatccatca aagccatggc cgacatgtac ggccaacacg agctcgaatt ggccaagctg
     3841 cgtcgcaggc cggtttaaga agcatttttc accgtcgaca catcacaccc aaccaagtcc
     3901 catcatgtac ctaatcaatt aatatatcaa taatgccata atctagaaat gaaaaggaag
     3961 tgaatatgat ggataacgaa cggtttcaag acctggtatt gtcacacttg gccaatatta
     4021 ctcaagaatt aaccatgagt aaggccgata ttaccgagct caagtccgat gtcgcagggc
     4081 taaaaactga tgttgcaacg cttcaagcaa attatattga gttaaaagcc ggccaggaca
     4141 caatcatgac ccgagtcgat aaattggcag aggaattaac ggaactccgg caagaagtcc
     4201 ggcagggttt cgcggaacta aaggaatcca tcaaagccat ggccgatatg tacggccaac
     4261 atgagctcga attggccaag ctacgacgca ggccggtttg aaagaaccgc acgcccttcc
     4321 atcttgattt accccaggaa catccgcgcg tcttttc
//
LOCUS       DASVMB010000349         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_118652,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000349 DASVMB010000000
VERSION     DASVMB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118652"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF531_09925"
     CDS             complement(<1..242)
                     /locus_tag="VF531_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714330.1"
                     /translation="MESNRKFRAHVREIKEKIAPYVLLAPFLFWLIVFFGYAFIRTFY
                     FSFTDYNLFAAPHWVGLKNYINLFQDPQFGRAFHNT"
     gene            complement(342..1598)
                     /locus_tag="VF531_09930"
     CDS             complement(342..1598)
                     /locus_tag="VF531_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714331.1"
                     /translation="MKKWLTVLLIVAVVLTIGTAGLAKSEVVIRVSGYGGIDVDVFGQ
                     LMDRVSATLKAQGIKVAYEPVADDYVRTLMNSLSSGTGPDLFYLQQETAEGFIASNKI
                     EPLNNYLAASKISPKKDFIPSLISAYSKGGKIYGIAKDFNTLVLYYNKDLFDLAKVPY
                     PDENDTWDTLESKLAKVTNKDKQIYGMAVQPEVQRMGAFAVATGWNFFDKKGRTNLLD
                     PNFKRAFTWYTGLVKKGIGVVPSDVGQGWGGGAFTKEIAATCIEGAWLINSINNDAPN
                     MRYGTTLLPKDSKTGKRGTLIYSVAWAMNKDGKHKKEAFKVLEQLTSAESQQFILEHG
                     MALPSRAALINSPYFKQDTAAAKANYTVLLAASKDNAFPFVTGVWGNDWANQINEPLR
                     AVLTNSSSADDALQEAQKRIEELMKR"
     gene            complement(1676..2695)
                     /locus_tag="VF531_09935"
     CDS             complement(1676..2695)
                     /locus_tag="VF531_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714332.1"
                     /translation="MIVTIRDVAKNSGFSVTTVSHALNGYSDVSSETRQKIMEVAAEL
                     KYMPNRAAKSLVSKESHTIGVFILERMTLKHPFVFDVLSGIMDEAGEEYDVLLVGTQK
                     INSISDLHRLCAQRGVGGAVLMGLRIGNPILQEMESDSFPVMLIDIPVLGKRAAYVSS
                     DNQCGAKQALEYLYKLGHRNIAFINGHEEAMVSKERLLGYEKTLAEYDLAIKDTYVFH
                     GDFSKESAKLGVRALMERNSEITAIFAASDLMATGAIEELNAMGLKVPGDVSIIGFDD
                     QDFSVHTSPPLSTIRQKKYDFGRMAIQEMIAMFHDQDYQPAVHKLDTDLIIRDSTGPA
                     RSSGR"
     gene            complement(2810..3538)
                     /locus_tag="VF531_09940"
     CDS             complement(2810..3538)
                     /locus_tag="VF531_09940"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7714333.1"
                     /translation="MGVFDGDKLIANWRLATDKIKTVDEYGILIKNLFVDRNLPASQV
                     TEAVISSVVPPVTGLFENVVEKYFGVRWLVVGPGVKTGLVIKYDNPREIGADRVVNAV
                     AGVNLYGPPLIIVDFGTATTFCAIAPNGDYLGGPLAPGLGIASDALFQRTAKLPRVEL
                     VKPQTVIGKNTIHSMQAGLIYGYIGLVEGLITRIKAEMNCKPMVIATGGFAEIIASET
                     GMIDRVNSDLTLEGLRLISNLNKA"
BASE COUNT          916 a          954 c          735 g          941 t
ORIGIN      
        1 atcgtattgt gaaacgccct tccaaattgg ggatcctgga agaggttgat gtaattctta
       61 agcccgaccc aatgcggcgc ggcgaaaaga ttgtaatccg taaagctgaa ataaaacgtt
      121 cgaataaatg cgtaaccgaa aaagacgatt agccaaaaga gaaatggagc caaaagaaca
      181 tagggagcga ttttttcttt tatttcacgc acgtgtgcgc gaaacttccg gttggattcc
      241 atatcccctg atccctcccg tacataaaat gaggatacat aattacgttt ggccggataa
      301 atatccggcc aacgtaattt gagattgagc caggaaacgg attaccgctt cattaattcc
      361 tcaattcgtt tctgagcttc ttgcaacgcg tcatccgccg aactggagtt ggtgagtacc
      421 gcccgaagcg gttcgttgat ctggttggcc cagtcattgc cccaaactcc ggtaacaaac
      481 gggaatgcgt tatctttgga ggcagctaat aacactgtat aattggcttt ggcggcagcc
      541 gtatcctgct tgaaataagg agaattgatg agcgcagccc gactcggcag tgccatccca
      601 tgctccagaa tgaactgttg actttcggca ctggttaatt gctctaaaac tttgaaagcc
      661 tcttttttat gcttgccgtc cttattcatc gcccaggcca cagaataaat cagtgtcccg
      721 cgtttcccgg ttttggaatc cttgggaagc agggttgttc catagcgcat attgggtgca
      781 tcattattga tactgttaat caaccaggct ccctcaatac atgtggctgc aatctccttg
      841 gtaaaggcgc ctccgcccca accctgaccg acatcggaag gaaccactcc gattcctttt
      901 ttgaccaatc cggtatacca ggtaaatgcc cgtttaaaat tgggatcaag cagattggtc
      961 cggccttttt tatcaaagaa attccatccg gtcgccacgg caaaggcacc catgcgttga
     1021 acctcgggtt gcaccgccat gccgtatatt tgcttgtctt tattggttac tttggccaat
     1081 ttactctcca gagtatccca ggtatcattc tcatccggat agggcacttt ggccaaatca
     1141 aacaagtctt tattataata gagaaccaag gtgttgaaat ctttggcaat accgtatatc
     1201 tttccaccct ttgaataagc actgatcaag gatgggataa aatctttttt gggggagatc
     1261 ttggaagcgg ctaagtagtt attgaggggt tcaattttat tggaagcaat aaacccttcg
     1321 gcagtttctt gttgaagata aaacaaatcc ggtccggtcc cggacgataa actattcatc
     1381 agtgtacgga cataatcatc agcgaccggc tcgtaagcca ctttgattcc ctgggctttt
     1441 agggtagcgg agacccgatc catcaattgc ccgaagacat cgacgtcaat accgccatat
     1501 ccggaaaccc taatcaccac ttcacttttt gccaacccgg cagtaccaat ggtcaaaact
     1561 acggcaacta tcaacaatac tgttaaccat ttcttcatcg tcgcgaaatc ctcctttaac
     1621 taaaacgttt tggataaaag ataaaaaaat accctctccc cttcaagcca tcccatcacc
     1681 tcccgctact tcgcgccggt ccggttgaat cacgaatgat taaatccgta tccagcttgt
     1741 gaactgccgg ttgataatcc tggtcgtgaa acatcgcaat catctcctgg attgccatgc
     1801 ggccaaaatc atattttttt tgacgaatcg tactcagcgg agggctggta tgaaccgaaa
     1861 aatcttgatc atcaaagccg ataatcgata catccccggg aactttcagc cccatcgcgt
     1921 tgagctcctc gattgccccc gtagccatca ggtcacttgc agcaaagatc gcggtaatct
     1981 cggaattcct ctccattaat gccctgactc caagtttagc ggattcttta ctaaaatcac
     2041 cgtgaaatac ataggtgtct tttatcgcca gatcatactc agccaaagtt ttttcatatc
     2101 ccaacaagcg ttccttgcta accatcgctt cctcatggcc attgataaat gcgatattcc
     2161 tgtgccctag cttgtatagg tactccagag cctgtttggc cccgcattga ttgtcggaac
     2221 tgacataagc agcccgcttt cccaaaaccg gaatatcgat gagcatcacc ggaaagctat
     2281 ccgattccat ttcctgaagg atcgggtttc caatgcgtaa gcccatcaat accgcaccgc
     2341 ccaccccgcg ttgggcgcat aaccgatgca gatccgagat gctgtttatc ttttgcgtac
     2401 caaccagcag gacatcgtat tcttccccgg cttcatccat gatcccgctc aacacatcaa
     2461 agacaaacgg gtgcttgagg gtcatcctct ctagaataaa cacaccgata gtatgtgatt
     2521 ccttggagac caaactcttg gctgcccggt tcggcatata cttcagctcc gcggccactt
     2581 ccataatctt ttgcctggtc tcgctgctga catcggaata accattcagg gcatgagata
     2641 cggtagttac ggaaaatccc gaatttttgg caacatcgcg aatggttaca atcatcccgg
     2701 actccttttc atcttctcca aaacgttttg gataatatat tctatttgac aggttaccgg
     2761 aatatccttc ttcaatcttc gaattttgat cataaagttt ttgaaaactt catgccttat
     2821 tcagattgct aatcaaccgt aatccttcca aagtcaaatc ggaattgacc cgatcaatca
     2881 tcccggtttc actggcgatg atctccgcaa accctccggt tgcaataacc atcggcttgc
     2941 aattcatctc cgccttgatt cgggtgatca aaccctcgac caagccgata tacccataga
     3001 tcaatccggc ctgcatacta tggatggtat tctttccaat caccgtttgc ggtttgacca
     3061 actctacccg gggtagtttg gcggtgcgct gaaataaggc gtcgctggcg attcccagcc
     3121 ccggcgccag cggtcctccc aagtaatccc cattcggagc aatggcgcaa aaagtggtgg
     3181 cagtcccaaa atcgacgatg atcaaaggcg ggccgtaaag attaactccg gccacggcgt
     3241 taaccacccg gtctgccccg atctcccgcg gattatcata cttaatcacc aggccggtct
     3301 tgacccccgg gccgactacc agccacctga ctccaaaata cttttccacc acattctcga
     3361 acaatccggt gaccggcggc actacacttg agattactgc ctccgtcacc tggcttgccg
     3421 gcaaattccg gtccacaaaa aggtttttga tcaggatacc gtattcatca accgtcttga
     3481 ttttatccgt cgccaaacgc cagttggcga tgagtttgtc tccgtcaaac acgccgatta
     3541 ccgtct
//
LOCUS       DASVMB010000350         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_632302,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000350 DASVMB010000000
VERSION     DASVMB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632302"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..496)
                     /gene="ispF"
                     /locus_tag="VF531_09945"
     CDS             complement(8..496)
                     /gene="ispF"
                     /locus_tag="VF531_09945"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7714334.1"
                     /translation="MLVKTGLGQDSHAFEDKPSGKRLVLSGVFFDDCPALAGNSDADV
                     VLHAVTNAVSGVTGINILGEVSDRLCLEEGITDSRIYLAEALKYLNDFSLSHLSVSIE
                     CRRPRITPRIPEMKAVLAEVLGLSLGDIGITATSGEGLTPFGKGEGIQVLAIVTAVKG
                     DG"
     gene            complement(605..1285)
                     /locus_tag="VF531_09950"
     CDS             complement(605..1285)
                     /locus_tag="VF531_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7714335.1"
                     /translation="MFQPIQSSTVVQQIIDKITQSLIKGELKPGDRLPPEPELALQLG
                     VSRTSLREALKTLGGLGVLVAKKRGGTFIATSGSQSMLDPLLFNLIIEKGSTDELFEL
                     RVLLEVDAIELVIRKASDQDLEQLAAELDRFERCIPDGDLDHLAQLDLEFHLKLLRIT
                     QNSSFIRISQVVMQLFAYPIEKSLKKIGPQQVLANHRALYRAISERDLLKAKDHVIKA
                     FESSRQFL"
     gene            1438..>3655
                     /locus_tag="VF531_09955"
     CDS             1438..>3655
                     /locus_tag="VF531_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714336.1"
                     /translation="MAGEFNLSSALSVKIPEAQTANPGDFVTYVLQLVNHSGTPLNLE
                     VKYITPPHWNVLGDNAFILQQQNPYFFPITIQVPAEALADSEASIQIQFKLKGGPDLP
                     VFTVPVKVNPVSMIDFIVPQSIKTPYGTAAAYTITIANHGNTGEQFSVGCSSENGWHF
                     EIEPKIFRLDPGQFQEVRVTHQVPPYTDLGSDQLTLTFSWNSEQKAITLATQVENLPG
                     DLGQKFYIWQGQVNLYHPNLMDLKTYPSSSVFSLQGLLGPDSSAQIYASDILDPNKPW
                     YIDLKSASWETEAGLFPLTWPGLIEPVSSSGKLLLSDRKNDGIYSIYTWSTDQPGASV
                     PVGFEISLPDRSSYRILRQTLPDDSLTVAEWESRSEFHPGLEWSHTLAYNLSNPSNYG
                     YSLGLQGEAGERYWSSGFQSIHNYLTYPFQNRLSLSWGQTGSAKGLNANLQLKYDFGA
                     NTQFYQDYSLAANLRWLQNLYIILADKYHLLEGAPTSNNATIYLESGWNTEQYEHNWW
                     LNYSLDHETEGTTSYTKLYWLTDYSYNDHEDLLFNPQLIATSFPSINDGSKLGLGYHR
                     EWLYGPDLTVFGYAHLNPEISWSSDIELDWKIYQYSLTFRYTGSWREGRLVTDVCSLI
                     LNQDFILPLKRPLGTIEGLVYLDLNHNGIHDPGEPPAGNVSFYLDGESVRFNTDSDGH
                     FQIGGLDPGEHRITLDTKYEAVYQPINEKTTTTVKAYQNAKVEIPLTRTQNLIGSIY"
BASE COUNT          949 a          989 c          789 g          928 t
ORIGIN      
        1 aggatcttta cccgtccccc ttgaccgctg tgacgattgc cagcacctga atgccctcac
       61 cttttccgaa gggagtcagc ccttcaccac tggttgcggt aatgccaata tccccgagcg
      121 ataaccccaa tacctccgcc aaaaccgctt tcatctccgg aatccgcggg gtaattcgcg
      181 gccgccggca ttcaatgctg acagagagat ggctcagact aaaatcattt agatacttca
      241 aggcctcagc caggtatatc cggctgtcgg tgattccctc ttccaggcag agccgatcgg
      301 aaacctcccc taagatatta atcccggtca ctccggaaac ggcattggtc acggcatgca
      361 ataccacatc ggcatcgctg tttcccgcta aagccggaca gtcatcgaaa aatacaccgg
      421 agagtaccaa tcgctttccg gagggcttat cttcaaaggc gtggctatcc tggccaaggc
      481 cggttttgac cagcaaaacc ctttcccccg tttcttttca tatcaatttg aattaggcag
      541 atgaatcagg atattgattc ccgtatatgg cggcctgtac ggccactcga accatcaaat
      601 ttttctacaa gaactgccgc gaactctcaa aggctttgat cacatgatcc ttcgccttga
      661 gcagatcccg ttccgaaatt gcccggtata aagcacggtg attcgccaag acttgttggg
      721 gtccgatctt ttttaaggat ttctcgatgg gatacgcaaa tagttgcatt accacttggc
      781 tgatccgtat aaacgaggaa ttctgggtaa tccggagcag tttcagatga aactccagat
      841 caagttgggc caaatgatcc aaatcgccat cggggatgca tcgttcaaac cggtccaact
      901 ccgcggcgag ttgttccaga tcttgatccg aggccttccg gatgaccaat tcgatcgcat
      961 ccacttcgag caatacccgg agttcaaaga gttcatcagt ggagcctttc tcgatgatca
     1021 gattgaacaa cagcggatcg agcatcgatt gggaaccgga agtggcgatg aaagtccccc
     1081 cgcgtttctt ggccaccaat acgcccaaac cgcctaaagt ttttaaggct tcacgcaatg
     1141 aagtccggct tactcccaat tgcaatgcca actccggttc gggaggcagt cgatcacccg
     1201 gttttaattc acctttgatt aaactttggg tgattttgtc gataatctgt tgcactacgg
     1261 tactggattg aatgggttga aacaagggat cacctcaaaa attaaaaaag gaatccgcaa
     1321 acggatacag aaaattacaa tatggggata aggaaatttt caacaagatt cgctcatcta
     1381 ctctttattt cgcttatctt taccattttc ctgctaacct cggatcttta tccctgcttg
     1441 gcgggggagt tcaacctttc ctccgctctc agcgttaaaa tccccgaggc ccagaccgcc
     1501 aatccgggtg actttgtaac ctacgttttg cagctggtca atcacagcgg gactcctctc
     1561 aatctggaag tcaaatatat cactccgcca cattggaatg tacttggcga caacgcattc
     1621 attttgcaac agcaaaaccc ctatttcttt ccgataacga ttcaagttcc ggcagaggcc
     1681 ttggcagact ctgaggcatc catccagatc caattcaagt taaagggcgg tcccgattta
     1741 cccgtattta cggtcccggt caaggtcaat ccggtttcaa tgattgactt tatcgttccc
     1801 caatccatta aaaccccata cggaactgcc gctgcctata ccattaccat agcgaatcat
     1861 ggcaataccg gggaacaatt cagcgtcgga tgtagttccg aaaatggctg gcattttgag
     1921 attgaaccga aaattttccg gctcgatccc ggtcaattcc aggaggttcg agttactcat
     1981 caggttcctc cttacaccga cctcggctcc gatcaactga ccctcacctt ctcatggaat
     2041 tccgagcaaa aagcgatcac cttggccact caggtcgaaa atcttccggg ggatttgggt
     2101 caaaagtttt acatctggca aggtcaggtc aatctttacc acccgaatct gatggatctg
     2161 aagacctatc catcgagcag cgtattctcc cttcaaggcc tattgggacc cgatagctcc
     2221 gcccaaatat acgcctcgga tatcctggat cccaacaaac cgtggtatat agatctcaag
     2281 tccgcctcct gggaaacgga agcaggttta ttcccgctta cttggccggg cctgattgaa
     2341 cctgtaagct ccagcggcaa gctattacta tccgaccgga aaaacgatgg catttactcg
     2401 atttatacct ggagtaccga tcaaccgggg gcatctgtac cagtaggttt tgaaatcagc
     2461 ctgcccgatc gttcctccta ccgtatactc cgccaaactc ttcccgatga ttcattgacc
     2521 gtggccgagt gggaatcccg gtccgaattc cacccagggc tggaatggag tcatacactg
     2581 gcctataacc tgtccaaccc gtccaattat ggatattccc tgggattaca aggcgaggct
     2641 ggagaaaggt actggtcctc cggctttcaa tccattcaca attaccttac ttatccattc
     2701 caaaatcgtt tatccctatc ctggggccaa accgggtcgg caaaaggcct gaatgctaat
     2761 ctccaactca aatatgactt tggggctaat actcagtttt atcaggacta ttcccttgcg
     2821 gccaacttaa gatggcttca aaatctgtat attatattgg ccgataaata tcatctgctc
     2881 gaaggtgctc caaccagcaa caatgccacg atttatcttg aatccggatg gaataccgaa
     2941 caatatgaac ataactggtg gttgaattat tcattggatc atgaaacgga aggtacaacc
     3001 agttacacca agttatactg gttgaccgac tactcctata acgatcacga ggatctgtta
     3061 ttcaacccgc aacttatcgc gacttcattt ccctcgatca atgacggctc caaacttgga
     3121 ttgggatatc accgggaatg gctttatggc ccggatttaa ccgtcttcgg ctacgcccat
     3181 ttaaacccgg agatctcttg gagcagtgat atcgaactcg attggaagat ctatcaatat
     3241 tcgttgacct tccgctacac cggttcctgg agagaggggc gactcgtcac cgatgtttgt
     3301 agtctgattc taaaccagga ttttatactc ccgcttaagc ggccgctggg tacgattgaa
     3361 ggtctggtct acctggacct taaccataac ggaatccacg atcccggtga acctccggcc
     3421 gggaacgtct cattttacct ggatggagaa tcggtccgtt tcaataccga ttccgacggt
     3481 cattttcaga ttggcgggct agatcccggt gaacatcgta tcactctaga caccaaatac
     3541 gaggctgtct accagcctat aaacgaaaaa accacaacaa cagttaaagc ctatcaaaac
     3601 gcgaaggtgg agattccgct aacccgaacc caaaacctta tcggatccat ctata
//
LOCUS       DASVMB010000351         5661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_188719,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000351 DASVMB010000000
VERSION     DASVMB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188719"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VF531_09960"
     CDS             <1..139
                     /locus_tag="VF531_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7714337.1"
                     /translation="KAGAYGIQGKGAALVKKIDGCYFNVVGLPISQLVMMLHEFGVSI
                     W"
     gene            153..842
                     /gene="radC"
                     /locus_tag="VF531_09965"
     CDS             153..842
                     /gene="radC"
                     /locus_tag="VF531_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX7714338.1"
                     /translation="MEQRITIKDLPLEERPRERLIQFGAENLAASELIAILLRTGTAD
                     RTALNLAEELLGRFKDLRGLCRASLTEICESPGIGIAKAVQIKAAFELGRRVMTQSPV
                     TQPSIRMPRDIYELLKGSFQDLDREHFKVIHLNIRNQVLKVETTAIGILSSSPVHPRE
                     VFKEAIRMSSAAVILTHNHPSGDPTPSQDDLALTSRLRNAGELLGIPVIDHVIFGDTR
                     YVSLKERGQLV"
     gene            977..2017
                     /locus_tag="VF531_09970"
     CDS             977..2017
                     /locus_tag="VF531_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7714339.1"
                     /translation="MFKSILGRFAKDMGIDLGTANTLVYVQGRGIVLQEPTVVAIEKE
                     TNNIFAVGNEAKQMVGRTPGNIVAVRPMKDGVIADFDVTEKMLHYFITKAGKNIGVLS
                     PRIIIGVPSGVTEVEKRAVMDAGLQAGAREVFLIEEPMAAAIGAGLAVEDPTGNLIID
                     IGGGTTEVAVISLGGIVSSRSLRIAGDEMNEAISHYIKRNYNLMVGERTAEEIKKEIG
                     SAYPDGEEHSMEVRGRDLLTGLPKTITVTGHEIQEALAEPISGIVDAVKMTLERTPPE
                     LAADILDKGLVMAGGGALLRGIDRLLSEETGMPVQLAEDPLTCVARGTGIALERDYLS
                     LKKVLISNKKMA"
     gene            2105..3055
                     /gene="mreC"
                     /locus_tag="VF531_09975"
     CDS             2105..3055
                     /gene="mreC"
                     /locus_tag="VF531_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7714340.1"
                     /translation="MFQFFQNRRVILIALVFLLLTWVVFVTSHQRTNEGRVEYFLNTA
                     MTPLESIFNSCGRTVNESWKTITHLARLKEENDRLQDEVAGLKLRQLGLDSLKSENNR
                     LRAALKFQSEQPNELVTAEIISMNPSNWNGAFLINKGANFGLRRNMAVISPQGVVGRI
                     GEVRATTAEVILITDPRQGNYIGGIVNRTRDMVIITGGGIRGECTIQPAVDNYFSELR
                     INDLIVTSETSEVFPKGLPVGRVVGFKKRVNSMVTVAYIKPIVSLSRLETVYVIKTKY
                     DVVKTNETQQPNSAAAINSGETDSNGATTSGGSNDALVNP"
     gene            3036..3530
                     /gene="mreD"
                     /locus_tag="VF531_09980"
     CDS             3036..3530
                     /gene="mreD"
                     /locus_tag="VF531_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX7714341.1"
                     /translation="MRWLILSIGVVLSVAIQSTWLAALHLPAQVTPDLVLIMAISYGL
                     LRGPDAGLLFGLGAGLFVDLISGGGLIGVQALSKMAAGFSAGFMEKNIFKDNLLVPVI
                     AVFVGTLVCESFNILMYIAFNANYQFFSTLFFTILPLAVFNALLAPIVYHLLLRMERR
                     FSKE"
     gene            3596..>5661
                     /gene="mrdA"
                     /locus_tag="VF531_09985"
     CDS             3596..>5661
                     /gene="mrdA"
                     /locus_tag="VF531_09985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7714342.1"
                     /translation="MEQKFRFLAGLVIFVFVTLTIRLWYLQILEGATSMVKSRANQTR
                     TIKINAPRGIFYDRKGRILVSSRLSHNVSVVPDDIKNKPEELALLSRLLNIPKATLMD
                     KLKPDPKHPRNPYQYLPIAKDIPPDTAIKLLEEKFNLPGVEVDEVPVRWYRYGEIASH
                     LFGYIREINDDELKKYKDQGYRMGDIIGKTGLEKTYEDTLRGEDGGKVFEVDITGRSI
                     PLDKDKIDSVPGNNLRLTIDLTLQQAAEQALKDQFEYLQKYSKYHNAKSGAVIALDPR
                     NGNILAMVSEPGFDPNLFAGTMPQDAYQKIRSEQALLNRNTQGTFAPGSTFKPITVFS
                     ALMEHKVTVKDKFLCNGYDIVWGKKLPCWISTATSGPREHGWETVIDGLKNSCNVVMA
                     ALSRLVGPEKLAQYARFFGLGKPTGLNLYPQESSGLVPDPEWKKKRRPSGESFWSPVE
                     TGQLAIGQNALTVTPIQLAQVYAAIANGGKVYRPQVVSAITSPEGKVIKKFSRQLTMD
                     LNISPQDREIVQQGLVDVVNEGGTAGGAFSGFPLDIIPVAGKTGTAQHPPGDNDALFA
                     SYAPSNKPEIVVIVLIQQGGSGSGTAAPVARKILETYFADRIKRARAQQAAAKAQLLP
                     KATTVPADGTTTWVRKNPTSPTPAVRSTSGPAATIPKGTKEQPENPLTRPSTAPSEPE
                     GIKPPAT"
BASE COUNT         1413 a         1358 c         1516 g         1374 t
ORIGIN      
        1 taaagccggc gcttacggta ttcaaggtaa gggcgcagcg ctggttaaaa agattgacgg
       61 atgctatttt aatgtagttg ggcttccgat cagccaactc gtgatgatgc tgcatgaatt
      121 cggggtctcc atatggtaag gagttgtata ctatggagca gcgtatcacc attaaggatc
      181 taccgctcga ggagcgaccc agggaacgct tgattcagtt cggcgcggag aatcttgccg
      241 cttcagaact aatcgctatc ctattgcgga ccggcaccgc ggatcgaacc gcactcaatt
      301 tagccgagga attacttgga cgattcaagg atctgcgggg gctatgccgg gcttcgctaa
      361 ccgagatctg tgagtctcct ggaatcggga ttgcgaaggc agtccagatt aaagcggcgt
      421 ttgagttggg acggagagtc atgactcaaa gccccgtaac tcagccgtcg atccggatgc
      481 ctcgggatat ttacgaatta ttaaaaggga gttttcagga tctggatcga gagcatttta
      541 aggtaattca cctgaacatc cgcaaccagg tgcttaaggt ggagactacc gctatcggaa
      601 ttttgagctc atctccggtt catccgcggg aagtctttaa agaggcgatc aggatgagca
      661 gtgccgcagt gattttgact cataatcatc cgagcggcga tccaactcca agccaggacg
      721 atttggcctt gaccagccgg ctccggaatg ccggcgaact tttgggaatt ccggttatcg
      781 atcatgtcat ctttggtgat actcgatacg tgagtctcaa ggaacgcggt cagctggttt
      841 aaccttgatt tcaagcgcac caccaccgcg gaaaaaattg ccaggcggcc atgcaactag
      901 atagcccgcc ccgcgacaga tcggtaccta catactttac ttgcttaatt cactatgatc
      961 tgggaggaaa aatcagatgt ttaaatcgat cttaggccgc tttgcaaaag atatgggaat
     1021 tgatctcggc actgccaata ccttggtcta tgtccaaggc cggggtattg tacttcagga
     1081 acccacggtg gtggcgattg aaaaggagac caacaatatt ttcgctgtgg ggaacgaagc
     1141 caaacagatg gtaggcagga cacccggtaa tattgtcgcc gttcgcccca tgaaagacgg
     1201 agtaatcgcc gacttcgacg tcaccgaaaa gatgttgcat tatttcatca cgaaggccgg
     1261 aaagaatatc ggggtgttat cgccccggat catcatcggt gttccctccg gagtgactga
     1321 agtagaaaaa cgtgcggtga tggatgccgg tttacaggcc ggcgcgcgcg aggtttttct
     1381 aattgaggaa ccgatggctg ctgctatcgg agcgggactg gcggttgagg atcccacagg
     1441 gaatctgatc atcgacattg ggggaggcac cacagaggtg gccgttatct ccttgggcgg
     1501 aatcgtctcc agccgctcat tgaggattgc cggtgatgaa atgaatgaag ccatcagcca
     1561 ttatatcaag cgcaattaca atctgatggt gggtgaacgc accgcggagg agattaaaaa
     1621 agaaatcggg tcggcctatc ccgacggaga agaacattca atggaggtta ggggccgtga
     1681 tctcctgacc ggccttccga agaccattac cgtcaccggg catgagattc aggaagctct
     1741 ggccgagcca atctcgggaa ttgtagatgc ggtcaaaatg acgcttgaga gaaccccccc
     1801 ggaactcgct gccgatattc tcgataaagg tttggtgatg gcagggggcg gagccttgct
     1861 ccgggggatt gatcggttat tgtccgagga gaccggcatg ccggttcagc ttgctgaaga
     1921 tcctttgact tgtgtagccc gtggaaccgg aatagccctg gaacgtgatt atcttagttt
     1981 aaaaaaggta ttgatctcca ataaaaagat ggcgtaaact tagatttggc aagtaacttt
     2041 tgcatctgtc ggagccagtt aaatatttcg gtgttgttat ctttttttag agggtgacct
     2101 actcttgttc caattctttc aaaatcgccg ggtcatatta atcgctttgg ttttcctcct
     2161 cttaacctgg gtggtgtttg ttacatctca ccaacgcacc aatgaaggta gagtggaata
     2221 ttttttaaac accgccatga ctccgctcga aagcatcttt aattcttgcg gtcgtacggt
     2281 gaatgagagt tggaagacca ttacccatct ggcgcggctt aaagaggaaa atgaccggct
     2341 ccaagacgag gtcgccggcc taaagcttcg gcagctcggt ttggattcct taaaatccga
     2401 aaacaatcgt cttcgggcag ctttaaagtt tcaatccgaa caacccaatg aactagtgac
     2461 ggccgagatc atctcaatga accccagcaa ttggaatggg gccttcctca ttaataaagg
     2521 cgcaaatttc ggtttgcgcc ggaatatggc tgttatcagc cctcaaggtg tggtgggccg
     2581 gatcggagag gtccgggcta ccacagcgga ggttattttg attaccgatc cccgtcaggg
     2641 aaattacatc ggcggtattg tcaatcgtac ccgggacatg gtgatcatta ccggaggcgg
     2701 gatccggggt gaatgtacca tccagcctgc agtcgacaat tactttagtg aattacggat
     2761 taatgacctg atagtgacct cggaaaccag tgaggtgttt cccaagggat taccggtggg
     2821 ccgggtggtc ggttttaaaa aacgggtcaa cagcatggtt actgtggcct atattaaacc
     2881 gatagtcagc ctaagccggt tggaaacggt ttatgtaatc aagaccaaat atgatgtcgt
     2941 gaaaacgaat gaaactcagc agcctaacag cgccgcagcc atcaattccg gagaaacgga
     3001 ttcaaatggc gccaccacgt ccggaggttc aaacgatgcg ttggttaatc cttagtatcg
     3061 gggtggtttt gagcgtggcg attcagtcga cttggcttgc agcattacat ctgccggcgc
     3121 aagtcactcc ggatctggtc ttgatcatgg ccatctccta cggactatta cggggacctg
     3181 atgccgggct gctgtttggt ttaggggcag gactctttgt tgatctgata tccggcggtg
     3241 gtctcatcgg cgttcaagcg ctttccaaaa tggccgccgg ctttagcgcc ggttttatgg
     3301 agaagaatat cttcaaagac aacttactgg tcccggtgat cgccgttttt gtcggcacac
     3361 tggtctgtga atcctttaat atattgatgt atatagcctt taacgcaaat tatcaatttt
     3421 tcagcacctt attttttacg atcttaccgt tggccgtttt taacgccctg ctggcgccga
     3481 tagtgtatca tctgctattg cgtatggaac gtcggttcag taaggaatag accaaaggtg
     3541 ttcaattttc gggaaagata cggtacgcct tcataaggag gccgttttag actaaatgga
     3601 acaaaaattt cgttttcttg cgggtttggt catctttgtc ttcgttacct tgacgatccg
     3661 gctttggtat ctacagatcc tggaaggcgc caccagtatg gtcaaatccc gggctaacca
     3721 gacccgaacc atcaaaatca atgcgccccg gggtattttt tacgaccgga aaggccggat
     3781 tttggtatcc agccgattgt cccataatgt ctcggtggtt ccggatgaca tcaagaataa
     3841 acccgaggag ctcgccttac tcagccgtct gctgaatatt cccaaagcca ctttgatgga
     3901 taagttgaaa ccggatccca aacacccccg caatccctat caatacttgc cgatagccaa
     3961 ggatattccg ccggataccg ccatcaaact cttagaggag aaatttaact tacccggtgt
     4021 cgaagttgat gaggttccgg ttcgctggta ccgttacggg gaaatagcaa gtcatctttt
     4081 cggttatatc cgtgagatta acgatgatga attgaagaag tataaagatc aaggttaccg
     4141 aatgggggac atcatcggaa aaaccggcct ggaaaagacc tatgaggata cactacgcgg
     4201 ggaggacggc ggcaaggttt ttgaagtcga catcaccggc cgtagcattc cgctggataa
     4261 ggataagatc gattcggttc ccgggaacaa tctgcggttg accatcgatc tcaccttaca
     4321 acaagctgcg gaacaggccc tcaaggatca atttgaatat ctccagaaat actccaagta
     4381 tcataatgcc aagtccggtg cggtaatcgc actggatccc cgcaacggaa acatcttggc
     4441 gatggtcagc gaacccggtt tcgatccgaa tctgttcgcc ggtacgatgc ctcaggatgc
     4501 ctatcaaaaa atccgatccg agcaggcact cttaaaccgg aatactcagg gtaccttcgc
     4561 tcccggttca acctttaagc ccattaccgt cttttcggcg ttaatggaac ataaggtgac
     4621 tgttaaagat aaatttttgt gtaatggtta cgatatcgtc tggggtaaga agttgccctg
     4681 ctggatctca actgcgacct ccgggccgcg agagcatggt tgggagacgg ttatcgacgg
     4741 cctaaaaaat tcttgcaacg tggtgatggc cgcgcttagc cggttggtcg ggcctgagaa
     4801 actcgcccag tatgcccgct ttttcggttt gggcaagcct accgggctca acctataccc
     4861 tcaagagagt tcgggactgg ttcccgatcc ggaatggaaa aagaagagaa ggccttcagg
     4921 cgaatcgttc tggtctccgg ttgagaccgg acagttggcc atcggccaga acgccttgac
     4981 cgtgaccccg atacagctgg cccaggttta tgcggctatc gccaatggcg gcaaggttta
     5041 ccggcctcag gttgtttcgg caattacttc acccgagggg aaggttatca agaagttttc
     5101 ccgccaattg acgatggatc tgaatatctc tccccaagat cgggaaatcg tgcaacaggg
     5161 tttagtggat gttgttaatg aaggcggtac cgcaggcggc gcttttagcg gatttccgct
     5221 ggatatcatt ccggttgccg gtaagaccgg taccgctcaa catccgccgg gggataacga
     5281 cgcattattt gccagttatg ctccgtccaa taaaccggag atcgtcgtca tagtcctgat
     5341 tcagcaaggc ggatcgggga gtggtacggc tgccccggta gcgagaaaga ttcttgagac
     5401 gtatttcgcg gatcggatca agcgagcccg ggctcagcaa gcggcagcca aagctcaact
     5461 gctgccaaag gccaccacgg tacctgcgga tggaacgacg acttgggtaa gaaagaaccc
     5521 aaccagtccg acacctgcgg ttcgttcgac atctggccca gccgcaacaa ttcccaaagg
     5581 aaccaaggaa caacctgaaa acccgcttac tcggccatct acagcgccgt ctgagccgga
     5641 agggatcaag cccccggcaa c
//
LOCUS       DASVMB010000352         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_411429,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000352 DASVMB010000000
VERSION     DASVMB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411429"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1242
                     /locus_tag="VF531_09990"
     CDS             196..1242
                     /locus_tag="VF531_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010171696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7714343.1"
                     /translation="MMTKQIPNHAETVLVTGGSGFVASWCLVELLRRGFTVRTTVRSL
                     SKEQAVRAAISTVIDPRDRLTFFAADLTSDVGWAEAVAGCDYVLHVASPLGRDNPKNP
                     DELIVPARDGALRVLRAATNAGVKRVVITSACAAASPSSYSENRVTDESLWTDPEDNT
                     LNPYRKSKTLAELAAWQFMKEEHDGPTTLTTILPGAVFGPVLAADTVGSVQVIGRLLQ
                     GRFPAVPRLGFEVVDVRDLVDVHIRAMMSTQAAGERFIAVGEFMWMTDIAKTLRSKLG
                     IPASKVPARTMPDFVFRLLALFEPGLRSVMPGLGRKHRHTPEKAQRILGWRPRPAEET
                     LIDCAKSLMAHHVV"
     gene            1298..2428
                     /locus_tag="VF531_09995"
     CDS             1298..2428
                     /locus_tag="VF531_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7714344.1"
                     /translation="MEYVQYGKTGMKVSRFGLGCMRLPADEKEAIAMIRYAIDHGVNY
                     LDTAYIYKDSEWITGKALKDGYRNKTYLATKSPIWNIAKYEDFEKYLDEELLRLGTDH
                     IDVYLLHNMNHGNWEKVKKFDGLTFLDKMVQKGKIVHKAFSIHNTLKAFKEIADAYEW
                     EMFQIQLNILDEKQQVGVAGLNYGAAKGLAAVIMEPLRGGYILKYLPQEAQDLINQYS
                     EKRSLVEWCFRWLYNMPEVSVILSGTSSLQQLKDNLKIFEHSASNVMSEEDLNLIQMI
                     RKAYEAKKSIGCTGCEYCMPCPRGVAIPEIFKLYNSQHLMESHVIDKAVYQSSLVPAG
                     SGADQCVACGICAKHCPQNLTIPELLKRVHDEFMNYTYPTQR"
     gene            complement(2550..3056)
                     /locus_tag="VF531_10000"
     CDS             complement(2550..3056)
                     /locus_tag="VF531_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714345.1"
                     /translation="MVTLLVKIGFVYAGYEPFAKKLLDASGFSPTKVFKVETTNVPAI
                     LEQQTRVLAEKFGTQAKEVEWETRFNQVTGDILKRSGQKKVAQIKVLVQQNQQEFVKW
                     LGYDIVGVFSAGELSPAKVMEYSKLGPQLIIDNFHNPQGKPIVEVAKCDYVELINFPS
                     AKAPTLIV"
BASE COUNT          822 a          709 c          790 g          848 t
ORIGIN      
        1 taacagcggc aattaaaaca tgtacatttg atgaagctgc ccgcctgtcg cttattccaa
       61 ccggcgggcg gcttttactt tttgcgggat taggcaattt tttagcgata gtgttatatc
      121 tgcattaggt atttcaagtt ataatgatga acagcaaaga atctataagg aatatgttca
      181 aggaggtttc ttttgatgat gacaaaacaa attccgaatc atgctgagac tgttttagtg
      241 acgggtgggt ccggatttgt cgcaagctgg tgccttgttg agcttttaag gcgcggtttt
      301 acagtgcgta ccaccgtgcg cagcctgtca aaggagcaag cggtacgggc cgccatttcc
      361 accgttattg atccccggga tcgcttaacc tttttcgccg ccgatctgac ttcggatgtt
      421 ggctgggctg aggcggtagc aggatgcgac tatgtgcttc atgtggcctc tccgcttggc
      481 agggacaacc ccaaaaaccc ggatgaattg attgttcccg cacgtgacgg agcactgcga
      541 gtcttgcgtg ccgccaccaa cgccggggtc aagcgcgtag tcataacatc tgcgtgtgct
      601 gccgctagcc cgtcatcata cagcgaaaat cgggttactg atgagtccct ctggaccgac
      661 cccgaggaca acaccctgaa tccctaccgg aagtcaaaga cccttgccga gctggctgcc
      721 tggcaattca tgaaggagga gcatgatggt cccactacgt tgacgacgat cctgccgggt
      781 gcagtgtttg gtccggtatt agcggcagat accgtgggtt cggtacaggt gatcggccgc
      841 ctcttgcaag ggcgttttcc cgccgttccc cgtctggggt tcgaagtcgt ggatgtgcgt
      901 gaccttgtcg atgtgcatat ccgggcgatg atgtcaaccc aggcagccgg agaacgattt
      961 attgcggttg gcgagttcat gtggatgacc gacatcgcga agaccttgcg ctcgaagctt
     1021 gggatcccgg ccagcaaggt cccggcgcga accatgccgg attttgtgtt tcggctcctg
     1081 gcgctttttg agcctgggct tcgttccgta atgccgggac tggggagaaa acaccgccat
     1141 acgccggaaa aggcccagcg tatactgggg tggcgcccgc gcccggcaga ggaaaccttg
     1201 attgattgcg ctaagagcct catggctcat catgtggttt aacgacagat gtatttaaaa
     1261 cgatggctcg tataaaatat gaacgaggtg aaaccggatg gagtatgtcc aatatggcaa
     1321 aacagggatg aaagtatctc gatttggcct gggttgcatg cggttacctg cggatgaaaa
     1381 agaagcgatt gcaatgatcc gttatgctat cgatcatggg gtaaattatc ttgataccgc
     1441 ctatatttat aaagatagcg aatggattac gggaaaggct ttgaaggatg gctaccgcaa
     1501 caaaacttat ttagccacca aaagtcccat ttggaacatt gcgaaatacg aagactttga
     1561 aaaatatctg gatgaagaat tgctgcgact tggaaccgat catattgatg tatatctgct
     1621 gcataatatg aatcatggca attgggaaaa ggttaaaaaa tttgacggtt taacttttct
     1681 cgataaaatg gtgcaaaaag ggaaaatagt tcataaagct ttttcgattc acaatacctt
     1741 aaaggcattt aaagaaatcg cagacgccta tgagtgggaa atgtttcaaa tacagcttaa
     1801 tatcttggat gagaaacaac aagttggcgt tgcaggtttg aactatggcg cagccaaagg
     1861 tttggcggca gttatcatgg aaccgctccg aggaggttat atactcaagt accttccaca
     1921 ggaagcgcag gatttgatca atcaatattc tgaaaagcgc tccttggtag aatggtgttt
     1981 ccggtggctt tacaatatgc cggaagtatc cgtaatttta agtggtacaa gctctttgca
     2041 gcagttaaag gataatttga agatttttga gcattcagct tccaatgtga tgtcagaaga
     2101 agatttaaat ttgattcaga tgattcgcaa ggcttatgaa gcgaaaaaga gcattggctg
     2161 caccggctgc gaatattgta tgccatgccc ccgcggggtg gcaattccgg aaatattcaa
     2221 gttatataac agtcaacatt taatggaatc gcatgtgatc gataaggcag tatatcaaag
     2281 cagtttggtg cctgcgggtt ccggtgcaga tcaatgtgta gcttgtggta tttgcgccaa
     2341 acattgcccc cagaatttaa caattccgga attgttaaaa agggttcatg atgaattcat
     2401 gaactatact tatccgaccc aaagataata tcggacaact tccctatctg aagactcatg
     2461 ggaagtaatc atgtaacaat aaaccggctg cgcctgcatc gggcagccgg ttgtcttatc
     2521 ttttataacc cgagttttcc cgcatttcct taaacaatca aggtcggtgc cttggccgat
     2581 ggaaaattga ttaattccac gtaatcacat ttggcgactt cgacaatagg ctttccttgc
     2641 ggattatgaa aattatcaat aatcaattgc ggtcccaatt tggaatactc catcaccttg
     2701 gccggactca gctctccggc gctaaaaact cccacgatat cataacccag ccatttgaca
     2761 aactcctgct gattttgctg gaccaaaacc ttgatttgtg cgaccttttt ctgcccggac
     2821 cttttcagaa tatcgccggt tacctgatta aagcgggttt cccactccac ttctttggct
     2881 tgagtgccga acttctccgc caacactctg gtttgctgtt cgagaatggc tggcacattc
     2941 gtggtctcca ccttgaatac cttggttggc gaaaagccgc tggcgtcaag gagttttttg
     3001 gcaaacggct cataaccggc gtaaacaaaa ccgatcttca ccagcaatgt aaccatactt
     3061 gatatccatt tcccacagtt gattcgagtc agtaatcact ctatttcgag ccagctttcg
     3121 cggatgtttg aatttaagtt gacgttgggg gcgtaaaatc ctttctaac
//
LOCUS       DASVMB010000353         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_44232,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000353 DASVMB010000000
VERSION     DASVMB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44232"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VF531_10005"
     CDS             complement(<1..834)
                     /locus_tag="VF531_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEX7714346.1"
                     /translation="MSNKISMRQNRSQSQAPLLEELLRYTRRPLRPFHMPGHKAGLGL
                     EGIWKRLGFPARLDLTEISAFCWESSLQAAEQLAAQFYQADRSYFLVQGASQGILCGM
                     LGAFQPGDTVLVGRNCHLSVIHGLILGDLNPVFLEVDWVPEWGLPAGIRPDILAKALN
                     EHPDSKGLIVTNPTYQGIANRLHTYRVELGDRTLMVDEAHGGHLGWCGLNGYDAWEDA
                     DIWVQGTHKILGSLTQTGILHLREGRIDPDRVQRCLEWITTTSPSYILLAALDSNRRW
                     LF"
     gene            complement(1090..1296)
                     /locus_tag="VF531_10010"
     CDS             complement(1090..1296)
                     /locus_tag="VF531_10010"
                     /inference="COORDINATES: protein motif:HMM:NF022223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor G inhibitor Gin"
                     /protein_id="HEX7714347.1"
                     /translation="MTVPICFGCGGNAPDGLKIYGQYLCPDCEAQLIRSKAGKLDYQH
                     WIDCCRKLWEELKINLDDSDTSVE"
     gene            complement(1493..1759)
                     /locus_tag="VF531_10015"
     CDS             complement(1493..1759)
                     /locus_tag="VF531_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HEX7714348.1"
                     /translation="MSNGLILAYLVILALLYVLGKNFWKPLFVLFSLFFQGALGALGL
                     YLFNMVAAAWQVEVPLNPFNALFTGFLGVPGLLALLALRYWIKI"
     gene            complement(1835..2152)
                     /locus_tag="VF531_10020"
     CDS             complement(1835..2152)
                     /locus_tag="VF531_10020"
                     /inference="COORDINATES: protein motif:HMM:NF022165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HEX7714349.1"
                     /translation="MKTQSGQERKSFWRRFFKNFVQAFRWDEDPATQNTPDLLENLES
                     AQREWRYAKLYFNCVTDPDLIDHAIFHLGATERKYVYLLKCARETGLQIDDYQSNIKY
                     HPG"
     gene            complement(2343..2939)
                     /gene="recR"
                     /locus_tag="VF531_10025"
     CDS             complement(2343..2939)
                     /gene="recR"
                     /locus_tag="VF531_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7714350.1"
                     /translation="MLYPQPMNRLIHELSKLPGVGPKTAQRLAFHILTLSREEIDKLA
                     ESLVEAKQKLIFCETCGHLTEVSPCQLCSDPGRDQTLLCVVEEAKDVIAMEKTRVYKG
                     QYHVLGGAISPLEGIGPEDLRIKELLQRVAGGKFGEVIVATDPNVEGEATALYLSKIL
                     RPLGVKVTRLARGLPVGGDLDYADEITLGKAMEGRTEL"
BASE COUNT          832 a          802 c          712 g          728 t
ORIGIN      
        1 gaaaagccag cgacggttgg aatcaagagc agccagtaga atgtaagacg gacttgtggt
       61 agtgatccat tccaaacagc gttggacccg gtccgggtca atccgacctt cccggagatg
      121 gagtatccct gtctgggtta aagaacctaa aatcttgtgg gttccctgaa cccaaatgtc
      181 agcgtcttcc caggcatcgt aaccgttcaa cccgcaccaa cccagatgac ctccgtgggc
      241 ctcatccacc atgagggtac ggtcaccgag ttccacccga taggtatgaa gccggttggc
      301 aattccctga tacgtagggt tggtaacgat taagcctttg gagtccggat gttcattgag
      361 cgctttagcc aatatgtccg gccggattcc ggctggaaga ccccattcgg gaacccaatc
      421 cacctctaga aagaccgggt tcagatcgcc caagatcaag ccatggatca ccgataaatg
      481 gcaattacgg ccgaccagaa ccgtatcccc gggttgaaaa gcccctaaca tgccgcagag
      541 aatcccttgg gatgcccctt gcaccagaaa ataactccgg tccgcctggt aaaactgggc
      601 agccagttgt tctgcggctt gtagggaact ctcccagcag aaagcagaga tttctgtcag
      661 gtcgagtctg gctggaaaac caaggcgttt ccaaatacct tccagtccaa gcccagcctt
      721 atgccccggc atatgaaacg ggcgtaaggg ccggcgggtg tatcgtagca gctcctccaa
      781 gagcggagcc tgagattggg accggttttg tcgcattgaa atcttattcg acattttcag
      841 ggagctttcc tctttttccc taagagtaac ccggggcctg gagtatttcg ggggaacctt
      901 ctctatcatc atatggaaaa agatccgatt ctagctctca ttggttgaac cgggatatac
      961 atccagaggg aaacatcagg aaaagattaa atgccaggga aaaaccatcc ggggaaaagt
     1021 tcgggatggg agtgtataaa taaaaatacg gcacctaaat gccgaaatgt ttctacgtaa
     1081 aaaattaaat tattccactg atgtatctga gtcgtccaga ttgattttca attcttccca
     1141 aagcttacgg caacaatcga tccaatgctg gtaatcgagt ttaccggcct tgctccggat
     1201 taattgagcc tcgcaatcgg gacagagata ctgaccgtaa attttcaatc catcgggagc
     1261 gttaccgcca caaccaaaac atattggaac ggtcattgtt ttacctccgc tcgcttctta
     1321 actcctgcaa ggattccgca acaggccttc atgcagtgga tcctgttatt aacctatgcc
     1381 ctacagtgcg gattcgtgtc tgtcccggtt gaggtacttt agcggatacg agcgtggtcc
     1441 gattcttggg cgatgatttg aaacaaagaa aaggagccgc tgccaccctc ctctaaatct
     1501 tgatccaata acgtagcgcc agtaaagcca ataatcccgg aactcccaaa aatccggtaa
     1561 atagggcgtt aaatggattt aacggtacct caacctgcca agccgcggca accatattga
     1621 aaagatataa acccaatgcc cccaaagcgc cctgaaaaaa tagactaaaa agtacgaaca
     1681 acggtttcca aaaatttttc cccagaacat aaagtagcgc caaaatcaca agatacgcca
     1741 ggattaatcc gttcgacatg ttacaacccc cccttggtaa aattgtattg aaaaggggcg
     1801 gcctttagaa ggggttgtct acaaaaccaa aactttatcc tgggtggtac ttgatattgg
     1861 attgatagtc atcgatctgg agcccggtct cccgggcaca ttttaacaaa taaacatact
     1921 tgcgttcagt ggctccaaga tgaaagatgg cgtgatcaat cagatcggga tcagtgacgc
     1981 aattgaaata caatttcgca tatcgccact cccgttgcgc gctttccaga ttctccaaca
     2041 aatcgggggt gttttgggtt gccggatctt catcccagcg aaatgcttgg acgaaattct
     2101 taaaaaaccg acgccaaaag gatttccgtt cctgcccgga ctgggttttc atgattttcc
     2161 ctccatctat caatctttcg ccgtaagaac cgtcctggcc aaacctataa ttcatagggt
     2221 cacggcggaa atttactgcg agacgctgcc gtaatcgatt tgcaacagca acgacaataa
     2281 tattatcaac actggaagga aaatttatac atcggcatgt cgaaaccatg atattcttgc
     2341 agctacaact cggtccggcc ttccatcgcc ttacccaagg tgatctcatc ggcgtaatcc
     2401 aaatccccgc ccaccggtaa gccccgggct aatcgagtta ctttcacacc gagcggccgt
     2461 aagattttgc taaggtacag cgctgtcgcc tccccttcta cattgggatc ggtggcgacg
     2521 atcacttccc caaactttcc gccggccact cgctgcagaa gttccttgat ccgcaaatct
     2581 tccgggccga tgccttcaag cggtgaaatg gcgcctccaa gaacatgata ttgtcctttg
     2641 tagacccggg tcttctccat agcgataaca tcctttgctt cctcgactac acaaagcaaa
     2701 gtctggtccc ggccgggatc gctgcataac tggcatggag agacttctgt caaatggccg
     2761 cacgtctcgc agaagatcag tttctgcttc gcctcgacca ggctttccgc taacttgtcg
     2821 atctcttcac gggagagggt caggatatga aaagccagcc gctgggcggt tttcggtccc
     2881 actccgggca gtttggaaag ctcgtggatc aaacgattca tgggttgcgg atataacatg
     2941 aattcctcac ttaaaggaaa tattccgatc ctttggtgga acaaaacaga actgtgacaa
     3001 aaacatgaaa cccaaaatag tcacagttct tcgagagaat cgcatggtga atggcgcctc
     3061 agaaaagccc cggc
//
LOCUS       DASVMB010000354         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_633395,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000354 DASVMB010000000
VERSION     DASVMB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633395"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1642
                     /locus_tag="VF531_10030"
     CDS             407..1642
                     /locus_tag="VF531_10030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714351.1"
                     /translation="MMSISIETPRLVLRSFHKDDAGQVALNSQQPNVAYWMSDMILVD
                     EAEAESWIEWINGKCRSTQNMQVLAIERRQDGVCIGLIGFAPKKEIDGEVEILYAIAD
                     PYQGNGYATEAGRAIIWQAFEKVGLDMLSAIIKPSNTASRRVIEKLGFVYVNTRTLPY
                     DGKMMEFDYYRLYHIETLPNPEWDFGILPDAEEMGNFFNRRADGYEAHMNEIGHDSKV
                     YQRAMAALLQTDRQLKILDLGCGTGLELQYLFERIPNARVCCMDLSDEMLAILRDHYQ
                     EYKDQLEIVRDSYLTWAYPDAAYDYVISVNTMHHLEEEAKTGLYRKIHRSLKPGGMYV
                     ESDFMVDRAMMEQYQARYKRIINGLPVCKSGHYHIDIPFTVEIQKELLFKAGFNEVKV
                     FHENIRPRGSGAVLVAYKG"
     gene            1681..2475
                     /locus_tag="VF531_10035"
     CDS             1681..2475
                     /locus_tag="VF531_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7714352.1"
                     /translation="MSHAHVPKALTYGAQSNLSIFEDTMVEMTWVQVKESADNGDIVL
                     FPIGVIEEHGPHMDLSPDVYCSYLASRFLRQKLQQKGIHSIIAPPYYWGISNDVKKYP
                     GTFSVRPETFQAMLVDIFTSLNSWGFKYVFVLNAHGDPTHVDIIEQSIKTANDDTDLH
                     VYYLGKLDIEVENPPAFPSPREGRYEPDYHAGAIETAVMYTYYPQKVNTKLVKDLKPQ
                     NTWDPLGYCGDPANFSMEKTIIEFWKADSEMDALKIEAVLKKDGTK"
     gene            2472..3362
                     /locus_tag="VF531_10040"
     CDS             2472..3362
                     /locus_tag="VF531_10040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714353.1"
                     /translation="MTLHFISDYKNDPAYRLSFNELAGKTFGIDFEKWYQLGFWNDRY
                     VCYSFAEGNRIVANVSASRLDLIFKGQRFSAVQIGTVMTEAEFRGRGLAAQLMRRVLS
                     EYENQCDLIYLFANRSVLDFYPKFGFVPVQESRFTAEIGDPGRFSGGFRRLDLGNSND
                     LMILKRLIHERRPISPTIDVDQAQSILAWHCLNVFPRDLYYLDDLELLIIYKIQDDDA
                     GARLHLYDVIGARKPEFRPIFERVAHRDITKAVFYFTPHFEDLTIQCHPYEDPDDTLF
                     IKSASVRLTGEFAYPLTGHT"
     gene            3636..>5606
                     /locus_tag="VF531_10045"
     CDS             3636..>5606
                     /locus_tag="VF531_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX7714354.1"
                     /translation="MIKKTRLFIPLFMAFCLMFLWGVPQGTAASDSDLKYDFEKGDLG
                     GWIPRGQGVMLSPVTEAAQSGKASLMTTNRTANWHGPSLNVNSIFKKGGVYTITGFVK
                     LVSAPASPSTIKFTVEQKPAGGATGWKTVAEQTVADTEWVKLSGVYPVNQEMSDQLFY
                     VESSNPNEAFYVDHVTIEMTVAPPESAIETDIPALKDVFADCFAIGAAVEPDQLTGVQ
                     SELLKKHFNSIVAENAMKPGSIQPMEGVFSWTNPDRIVQFAIDNHMEIRFHTLVWHQQ
                     VGEWFFKDPDGKDMTLETDPAKREANKKLLLQRLEKHITAIAERYQGKIKSWDVVNEV
                     IDPSQPNGMRNNTWYQIAGADYIETAFRIVRKVAGPDAKLYINDYSTHDPAKRDLLYK
                     LVKEMLAKGVPIDGVGHQMHMNINDPSIALIDQSIKLFAGLGLDNQITELDVSVYTND
                     TASYQQVPGEIINLQGHRYRELFDELRKLKDSISNVTFWGFTDEHTWLHNRPVPRRDA
                     PLPFDQKYQAKPAYWGMVDPGKLPVLSQNLDIYRGTPVIDGVTEQSWQILNWTPVKLG
                     NGSLAAAFKACWDEQHLYVLADINDATRNSNDSVEIFCDQDNGKTETYQAGDTHYILK
                     RGAKSSGDMVYKVKEYKGGYILEASLPLTAGSI"
BASE COUNT         1496 a         1225 c         1471 g         1414 t
ORIGIN      
        1 aaaacgggct gggaagaact ggatgtatta cccagcccca ttcatggggc ttgagatgat
       61 ttttccgggc gtagccaggt cctttagggc ctggtgggga taaggacgat tatccattgt
      121 ttgcttttga gacagccccg ataggggtgg ttgacccaat gaatagctaa aatgggattt
      181 aaatttacac aattagctaa aattccataa ggaggtattt ccatgccggc gaccgacctg
      241 tcttacctga tttcataagg gaggacgatc tgctcttccc gaacgcagga gggcaggaaa
      301 atgactacaa agatctcaat cttgctttag gtttggagac gggtctgata ttcgaatgag
      361 ttgaatatca gactagtcag atattcaata tcgaaaggag ttttttatga tgtctatttc
      421 tattgaaaca ccccgtctgg ttctgcgcag ttttcataaa gacgatgccg ggcaggtggc
      481 cttgaacagt caacaaccga atgtcgccta ctggatgtcg gatatgatcc ttgtggatga
      541 agctgaggca gaatcatgga ttgaatggat caacggtaag tgtcggtcaa cacagaatat
      601 gcaggtttta gccattgaac ggcggcaaga cggggtctgc atcggcctaa tcggttttgc
      661 gcccaaaaag gagatcgatg gtgaagtgga aatcctctat gctatcgccg acccgtatca
      721 ggggaacgga tatgctaccg aggcaggccg ggccatcatc tggcaggcgt ttgagaaagt
      781 aggccttgac atgttgtctg caatcattaa gccatccaat actgcttccc gaagggtcat
      841 tgagaaactg ggctttgtct atgtaaatac ccggacacta ccctatgacg gaaagatgat
      901 ggagtttgat tattaccggc tctatcacat cgagacttta cccaatccgg aatgggattt
      961 tggcatcttg ccggatgctg aggagatggg gaattttttt aaccggcgcg ccgatggcta
     1021 tgaagctcat atgaatgaga taggccatga ttccaaggta tatcagcgcg caatggcggc
     1081 tcttcttcaa accgaccgac agctaaaaat tcttgacctc ggctgcggta ccggactgga
     1141 gcttcaatac ttgtttgagc gaatccccaa tgcccgggtt tgctgtatgg atctttccga
     1201 tgagatgctg gccatattaa gggatcatta tcaggagtat aaagatcagt tagagatagt
     1261 tcgcgactcc tatctgactt gggcgtatcc cgacgccgca tatgactatg tgatctccgt
     1321 caataccatg caccacttgg aagaagaagc gaaaaccggg ctttaccgca agattcaccg
     1381 tagtcttaaa ccggggggca tgtatgtcga gtcggatttt atggtagatc gggccatgat
     1441 ggaacagtac caagcgcgtt acaagcggat catcaatggt ttgccggttt gcaagagcgg
     1501 acattatcat atcgatattc cgtttacggt agagattcag aaggagctgc ttttcaaggc
     1561 tggttttaat gaagtgaagg tgttccatga aaatattcga cccagaggca gtggtgcggt
     1621 tctagtagcc tataagggtt agtctaaaga aattttaaaa atctgaaaga ggtggcaacg
     1681 atgagtcacg cgcatgtgcc aaaagcattg acttatggag cccaatccaa cttatcaatt
     1741 tttgaagata caatggttga aatgacctgg gtacaggtta aagaatcggc agataacggg
     1801 gatatcgtct tattccccat cggcgttatt gaggaacatg gaccccatat ggatttgagt
     1861 ccggatgttt attgttccta tttggcttct agatttctgc gacagaaatt gcagcaaaag
     1921 gggatccatt cgattattgc accgccgtat tattggggaa taagtaatga tgtaaaaaaa
     1981 tatccgggta cattttcagt caggccggaa acatttcaag cgatgttggt cgatattttt
     2041 acttctttga attcatgggg tttcaagtat gtttttgttt tgaatgccca tggagaccct
     2101 acccatgtag atatcatcga gcaatccata aaaacagcaa acgatgatac ggacctccat
     2161 gtgtattatc tgggaaaact cgatattgag gtggaaaatc cgccggcttt cccctcgcca
     2221 cgagaaggta gatatgaacc ggattatcac gccggggcta ttgaaacagc tgtcatgtat
     2281 acatattacc ctcaaaaggt gaatacaaaa ttggtgaagg atttaaagcc gcaaaacaca
     2341 tgggatccat taggctattg cggtgatccg gcgaactttt ccatggaaaa aaccatcatt
     2401 gagttttgga aggcggattc ggaaatggat gcattgaaaa ttgaagcagt cctaaagaag
     2461 gatggtacaa aatgacgtta catttcatca gcgactacaa aaatgacccc gcttaccgct
     2521 taagctttaa tgaattagcc ggtaagactt ttggaattga ctttgaaaaa tggtatcagc
     2581 ttggtttttg gaatgaccgg tatgtatgct attccttcgc ggaggggaac cggatcgtgg
     2641 ccaacgtctc cgccagcagg ctggatctta tttttaaggg gcagaggttc agtgcggttc
     2701 agatcggaac ggtgatgacc gaggccgaat tccggggaag ggggcttgcg gcccaattaa
     2761 tgcggagggt tctaagtgag tacgagaatc aatgtgatct gatttatctc tttgccaacc
     2821 gttccgtcct cgatttctat cccaagttcg gctttgttcc ggtacaggaa agccggttca
     2881 ctgctgaaat tggggatccg ggccgttttt ccgggggctt ccggaggctg gatcttggga
     2941 acagtaatga cttgatgatc ctaaaaaggc tgatccatga gcgacgcccc atatccccta
     3001 cgatagatgt ggatcaagcc cagtcgatcc tggcctggca ttgcctgaat gtgtttcccc
     3061 gggatctcta ctatctagac gatctggagt tattgatcat ctataaaatt caggatgatg
     3121 atgcgggggc gcgacttcac ctctatgatg tgatcggggc aaggaagccg gagttcagac
     3181 cgatttttga aagagtggcg caccgggaca tcaccaaggc ggttttctat tttacccccc
     3241 actttgagga tctgactatc caatgtcatc cctatgaaga tccggacgat acccttttta
     3301 tcaaatctgc ttcagtccgc ttgaccgggg aatttgccta tcctttgacc ggacatacct
     3361 aagagtaaaa aaggtttgcc gcggagccgc catccggtga ctgccgcggt ttttttgtgt
     3421 cattccggtg atcaagttag cggtaccgtt cacgttatga gcggtcggtc ctagatccca
     3481 taattttcta atatgatata attgagacaa atgaatgctt aatttatagt ttcgggaaaa
     3541 catcggttat cgcaacgaaa aggaagatga tgacgtactt cgtggccgga ttgaatgggg
     3601 gaagcgtgaa agagtgacta aggagggtaa tgggtatgat caaaaaaacc aggttattca
     3661 tcccgctatt tatggcattt tgtttgatgt ttctatgggg agtgccgcaa gggactgcgg
     3721 caagtgacag cgatttaaaa tatgactttg aaaaaggtga tctcggagga tggatcccgc
     3781 gggggcaggg ggtaatgtta tccccggtca cggaagcggc ccagtccggt aaggccagtt
     3841 tgatgacgac caaccgtacg gccaattggc acgggccgag cttaaatgtg aacagtatct
     3901 ttaaaaaggg cggcgtatat acgatcactg gtttcgtcaa gttagtgagc gctccggctt
     3961 ctccaagtac catcaagttt acagtggagc aaaaaccggc cggcggagca accggctgga
     4021 agacggtggc agagcaaacc gtggccgata ccgagtgggt caaactctcc ggtgtctatc
     4081 ccgtaaatca agagatgagc gatcaactat tctatgtgga gagttctaat ccaaacgagg
     4141 ctttttacgt ggatcatgtg acgattgaaa tgactgtggc tccgccggaa agcgccatcg
     4201 aaaccgatat tccggcactc aaggatgttt tcgccgattg tttcgccatc ggagcggcgg
     4261 ttgaacccga ccagctgacg ggggttcaaa gcgaattgtt aaagaaacac tttaatagca
     4321 tcgtggcgga gaatgccatg aagcccggtt cgatccagcc gatggagggc gttttcagct
     4381 ggaccaaccc cgaccgcatt gttcagttcg ccatcgataa tcatatggag attcgttttc
     4441 ataccctggt ctggcaccaa caggtggggg aatggttctt taaggatccc gacggtaaag
     4501 atatgacgct tgagacggac ccggcgaaac gggaggccaa caaaaagtta cttctgcaaa
     4561 ggttggagaa acatatcacc gccatcgccg agcgatacca gggcaagatc aagtcctggg
     4621 atgtagtgaa cgaagtgatc gatcccagcc agcccaatgg gatgcgcaac aatacgtggt
     4681 accagatcgc aggagccgac tatatcgaaa ccgctttccg tattgtccgc aaagtggccg
     4741 gacccgatgc caagctctat atcaacgatt atagcaccca tgatccggca aaacgcgacc
     4801 tcctgtataa actggtcaag gagatgctgg cgaagggagt gcccatcgac ggcgtcggac
     4861 atcagatgca tatgaatatc aacgatccat ccatcgcttt gatcgaccaa tccatcaaac
     4921 tctttgccgg attgggcctg gataaccaga tcaccgagtt ggatgtgagc gtctatacga
     4981 acgatactgc ttcctatcag caagttccgg gcgagatcat caacttacag ggtcaccgtt
     5041 accgggagtt attcgacgaa cttcgcaagc tgaaagattc catcagtaat gttaccttct
     5101 ggggcttcac cgatgaacac acctggttgc acaaccggcc ggttccccgt agggacgcgc
     5161 cattaccctt tgatcaaaag taccaggcca aaccggctta ctggggaatg gtggatccgg
     5221 ggaaactgcc ggttttatct caaaatctcg atatctaccg gggaacaccg gtcattgacg
     5281 gtgtcaccga acaatcatgg cagatattaa actggacgcc ggtaaaattg gggaacggtt
     5341 cacttgcggc agcttttaag gcttgctggg atgaacaaca cctttatgtg ttggcggata
     5401 tcaacgatgc cacccgaaac tccaatgact cggtggagat cttttgcgat caggataacg
     5461 gcaaaaccga gacttatcaa gcgggggaca ctcattatat cctgaagcgc ggcgccaaat
     5521 cctccggtga tatggtatat aaagtcaagg aatataaggg tggttacata ctggaagcct
     5581 cgctgcctct tacggcgggt tctatc
//
LOCUS       DASVMB010000355         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_267299,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000355 DASVMB010000000
VERSION     DASVMB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267299"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VF531_10050"
     CDS             <1..364
                     /locus_tag="VF531_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX7714355.1"
                     /translation="DKIPSGFTDKVFELRYSVRKGDTLTAIARRYGVNLATIVTRNKL
                     GNSGKIKPQAELVIPIPGERIYILGAGETLWRVAKRYGTTVELLQDLNQITDIRKIRA
                     GQPIVLPVTVREIKNPQF"
     gene            378..647
                     /locus_tag="VF531_10055"
     CDS             378..647
                     /locus_tag="VF531_10055"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEX7714356.1"
                     /translation="MKNYMEDVVLAVYNEFKVKYPKFCSCERCKADTVAIALTKLKGR
                     YAVSPEGEIFARLSRDDRQVRADALMVLMEAAEIVAKQPNHNKQF"
     gene            complement(654..887)
                     /locus_tag="VF531_10060"
     CDS             complement(654..887)
                     /locus_tag="VF531_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714357.1"
                     /translation="MMDIKSSKPGNSVIQECPEYHAIQTLGHPASEYNTGDRITDYTV
                     MNDAIINDEGEETGCDSCIHFNRGNCGAFNRKN"
     gene            complement(1009..1170)
                     /locus_tag="VF531_10065"
     CDS             complement(1009..1170)
                     /locus_tag="VF531_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714358.1"
                     /translation="MSEEEELENRVQGCRGFEPVLVNSNSESSATDKTASDCGSCVYF
                     DNMECKVLK"
     gene            complement(1411..1962)
                     /locus_tag="VF531_10070"
     CDS             complement(1411..1962)
                     /locus_tag="VF531_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714359.1"
                     /translation="MQIRKSIIFVLVFLLIAGFGAVQAGAAQSSTPSTVYENSAAGIR
                     IEYPQNWELQEGVMGTLASFLSPVEDSSDIMRENANIVVQDLSKQPMTLDQYTELSIT
                     QLKQLITDFHEETSEKTTWAENPAYLLVFSGKQGQYDLKWKMIWTIKNNVAYVVTFTA
                     QAEKYDAFLAQADQIFGSFTILR"
     gene            complement(2086..>2487)
                     /locus_tag="VF531_10075"
     CDS             complement(2086..>2487)
                     /locus_tag="VF531_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX7714360.1"
                     /translation="YFDDSFATAPDPTMVALKAFHEPIVLIAGGADKGADFSGLAEAI
                     LKSTVHTVILIGLMGPKIGSAILQTAAKLGLPSPRLINGGPDMESIVSTASAQAASGE
                     IVLLSTACASFGMFKNYKERGTLFKQAVMKL"
BASE COUNT          674 a          555 c          593 g          665 t
ORIGIN      
        1 cgacaagatt ccatcgggat ttaccgacaa agtttttgaa ttacggtata gtgttcgtaa
       61 aggcgatacc ttaactgcca ttgcccggcg ttacggggtg aacctggcga cgattgttac
      121 ccggaacaaa ctagggaatt cagggaagat caagcctcag gcagaactag tcattccgat
      181 tcccggagag cggatttata ttcttggagc cggggagacc ttatggaggg ttgccaagcg
      241 ttacggcacc accgttgaat tgctgcagga ccttaatcag ataacggata ttcgaaaaat
      301 ccgggctggt cagcccattg ttttaccagt gacggtacga gagattaaaa atccgcaatt
      361 ttaaggggtt gatttggatg aagaattata tggaagacgt ggttttggca gtttataatg
      421 aattcaaggt caaatacccc aaattttgtt cgtgtgagcg ctgtaaggcc gatactgtcg
      481 ctatcgccct gaccaaactg aaaggcagat acgcggtaag cccggaaggg gagatttttg
      541 ccagattgtc gcgggatgac cgtcaagtcc gggcggatgc cctcatggtt ttgatggaag
      601 ctgccgagat agttgcaaag caacccaacc ataacaaaca attctaagcc gcattagttt
      661 ttacggttaa atgcaccgca attgccacgg ttaaaatgga tgcaggaatc acaaccggtt
      721 tcctcacctt catcatttat gatggcatca ttcatgacag tgtaatccgt gattcgatcc
      781 ccggtgttat actccgatgc cggatggcct aaggtttgaa tagcatggta ttccgggcat
      841 tcctgaataa cggaattgcc aggtttgcta ctttttatat ccatcaatct tatccctcac
      901 ttgcgaattc gacacttaaa aatattcccg gcggatagaa acaaggattt ccaccgggaa
      961 agcgttttta cggagtgtag ttctaacaag ccactctaaa aagggacctc attttaagac
     1021 tttgcattcc atattgtcaa agtatacaca cgagccgcaa tcagatgcgg ttttatctgt
     1081 ggcagagctc tccgaattgc tgtttaccaa aaccggttcg aagccacggc aaccctgaac
     1141 ccggttttcc agttcttcct cttccgacat tcacttcacc tcgttgaccc tgcaaaataa
     1201 gttgggaagc gaaattagtt taacgccgtc ctggtacgat tattcactgg agtttcatgg
     1261 ttctaagcag cataaaaagc gggtgggata atccgaagtt caatgatatt aatggtcttc
     1321 ggggaatacg gcttcaaaat aaaaatacct ttcccgaaag ggggaaaggt aggtgaatct
     1381 tcattgattg gcgggcgacc agggttggcc tcagcgaagg atggtgaaag agccgaagat
     1441 ctgatcggct tgggccagga aagcgtcata cttctctgcc tgagcagtaa aagtcaccac
     1501 ataggccaca ttgttcttga tcgtccagat cattttccat ttcaagtcat actgtccttg
     1561 tttgcccgaa aaaaccaata aataggccgg attttcagcc caagtcgttt tctcggaggt
     1621 ttcctcatga aaatcagtaa tcagttgttt taattgagtg atgctaagct cggtgtattg
     1681 atccaaagtc attggctgct tacttaaatc ctgaacgacg atgtttgcat tttcccgcat
     1741 aatatcactg gaatcttcca ccggggatag gaatgaagcc aaggtaccca tgaccccctc
     1801 ctgcaactcc cagttttgcg gatattcgat ccgaatgccg gcggcagagt tttcatagac
     1861 agtggatgga gtagacgatt gggcggcgcc ggcttgtacc gccccaaagc cggcaataag
     1921 caggaaaacc agaacgaaga tgatggattt acgaatttgc aacttaggaa cctcccgtaa
     1981 tttgaattga atggatttta tttttttgac cgtggtacct tttttatgaa attcattcca
     2041 tgaaataatt tctagctggg gtagcaattt ccttccgaga aaaaactaaa gtttcattac
     2101 tgcctgtttg aaaagggttc cccgttcctt ataatttttg aacataccga agctggcgca
     2161 ggctgttgat aatagaacta tttcacctga agctgcctgg gcggaggctg ttgagacgat
     2221 actttccatg tccggcccgc cattaatcag ccgggggctg ggaagaccaa gttttgcggc
     2281 ggtttgaaga atggcggatc cgatcttcgg acccatcaat ccaatcagaa taacggtgtg
     2341 gacggtactt tttaaaatcg cctcggctaa accggagaag tcggcacctt tatcggcccc
     2401 tccggcgata agcacgatcg gttcatgaaa agcctttaac gccaccattg tcggatctgg
     2461 cgctgtagca aaagaatcat caaaata
//
LOCUS       DASVMB010000356         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_559808,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000356 DASVMB010000000
VERSION     DASVMB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559808"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2196)
                     /locus_tag="VF531_10080"
     CDS             complement(<1..2196)
                     /locus_tag="VF531_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7714361.1"
                     /translation="MSDGILTHFGRWLIRRRWWVLAAWCLLVLGGIYCTPRVDGILRG
                     DTKIPGGEAAEVTELLKNNFGNRFGYELVVVFHSKTRNYDEPEYQAAVEAVQLGLRDS
                     LKVNTVYTYYDTNSADLASKDHHSILGLVELDVENVFNSQQAVPEIRRIIAGTKIPKW
                     LTVYVTGEGATYYDMIHSSARDAFWAESRSFPIVLVILVFTFGALVASGIPVILGLVA
                     VSITMGVVYLMGMHTSMFIIAKNAVTMIGLGVGIDYSLFMVSRFREELKNGMDPHEAA
                     VAIMSTSGKTVLFSGTTVVIGLASVFLAGMPFLDSMAAAAIAVVVVSVLAALTLLPVL
                     FYWMGERINWPKSLSGLISRSKSGRVWHRLATRVMKYPVAFFLGTLAILLLASLPLSH
                     IKTYTPTVVGLPRGVESRLGFEQLQADFVAGKMSPIDIVVEAPAGQSIWSRESVTRIY
                     RFSRLIGSDRRVSKVEGIVDVDPKLTLADYLKLYCGSEGISGSDNIFAQMIGSYVDNS
                     GQGRQTLLRVTTVPEPGSMESRALVKAIRAELVSKAFGGSGYRILVGGGSANDVDLDQ
                     SLMGKLPLIIGLVCVITFIILMILFRSILIPLKSIFMNVLSVLASFGLLVLTFQDGAI
                     TYLTGLVAPGGISSVILVMLFAVLFGLSMDYEIFLTLRIKEEHDNGNNNEESVAWGLE
                     RTGGLVTSAALIMVAVFGAFVFTSMMLIQEFGLGLAAAVFLDATIIRVLL"
     gene            complement(2246..3448)
                     /locus_tag="VF531_10085"
     CDS             complement(2246..3448)
                     /locus_tag="VF531_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714362.1"
                     /translation="MKRFMALILSVLFLLGSFTVVLADEAAGTTIDYSGVLEVGGVSQ
                     SHNTDYYLLSVVPNALDNIPTMGNSIKLRNDLRLIPNDQTTVRLRVSYLRDTIQNPND
                     QGSFSLSRGFIDFTPSDQFSMRLGKQRLAWGTGYAWNPTDILDIPRNAFTDSDDPDGI
                     MAYRGDLNLGPVAAQAVITPQDSWENSGRALRFKVSPAGVDLTLGLAQNGTDSAATTA
                     DFAYSLAGVGIHGETLYQAKGNRRSDKKDILNYLVGADYNLPGGYYLALEYYHNDQAY
                     QDATEFKNYSTNLLYQAGLDTAKLAAASTEIQTTMVNLANNGGITRDHFFIRATKGFG
                     DNINTELLLIVNPGDGSLVGQPLFEYTWQQNTKMFVKGMLATGGTDSEANLLPTKAQW
                     NLGLKVNF"
     gene            complement(3871..>4070)
                     /locus_tag="VF531_10090"
     CDS             complement(3871..>4070)
                     /locus_tag="VF531_10090"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7714363.1"
                     /translation="GGGRLVKEATQTQITTMDGRQVPTKMIFKDLEQKGHQTILTINS
                     IRFTEDIQERYFSMQYLEKGQ"
BASE COUNT          991 a         1144 c         1028 g          907 t
ORIGIN      
        1 caataacacc cggatgatcg tggcgtcgag aaagacagcg gcggccaacc ccaggccgaa
       61 ctcctggatc agcatcatgc tggtaaagac gaaggcgccg aaaaccgcca ccataataag
      121 agcggcgctg gtcaccaggc ctccggtgcg ttccaggccc caggcaacgc tctcctcatt
      181 gttattgccg ttatcgtgct cctccttgat ccgcagggtc aggaagatct cgtaatccat
      241 cgaaagtccg aatagaactg caaaaagcat caccaggata acgctggaga ttcctccggg
      301 agccacgagg ccggtcaagt aggtgatcgc accgtcctgg aaagtcagga ctaacagtcc
      361 gaaactggcg aggaccgaaa gcacattcat gaagatggat ttgagcggga ttaaaatcga
      421 acggaacaga atcatcagga taatgaaggt aatcacgcaa accaatccga tgattaaagg
      481 aagttttccc atgagcgatt gatccagatc cacatcgttg gcggatccgc cgcccaccaa
      541 gatccggtag ccggaaccac cgaaggcctt cgagacgagc tctgcccgaa tcgccttgac
      601 cagtgcgcgg gattccatgg aacccggttc ggggacggtg gttactcgca gcaatgtttg
      661 ccggccttga ccgctgttgt ccacgtatga tccgatcatc tgggcaaaga tattatccga
      721 tccggaaata ccctcgctac cgcagtaaag tttcaaataa tccgccaggg tcaacttggg
      781 atcgacatcc acgattccct ctaccttgga gacccgccgg tcacttccga tcagacggga
      841 aaagcgatag atcctggtaa cggactcccg cgaccagatg ctttggcctg ccggagcctc
      901 cacgacgatg tcaatgggcg acatctttcc ggcgacaaaa tcagcctgca gttgctcaaa
      961 gcccaaacgc gactcgacac cgcgaggcaa cccgaccacg gtcggggtat aggttttaat
     1021 atgggaaagc ggcagggagg ccaaaagcag gatggccagg gtgcccagga agaatgctac
     1081 cggatatttc ataacccggg tagccagtct gtgccagacg cggccgctct tggagcggct
     1141 gatcaaaccc gagagcgact taggccagtt aatccgctcc cccatccaat aaaagagcac
     1201 cgggagcaag gtaagagcgg ccagaaccga tacaaccaca acagcgatgg ctgctgcagc
     1261 catggagtca aggaagggca ttcccgccaa aaataccgag gcgagtccaa tcactaccgt
     1321 ggtcccggag aagagcaccg tcttgccgga ggtggacatg atggccaccg ccgcctcatg
     1381 agggtccatc ccgtttttca actcttcccg gaaacggctg accataaaca gggagtaatc
     1441 gatccccacc cccaaaccga tcatcgtcac cgcgttcttt gcaatgatga acatcgaagt
     1501 gtgcatcccc atcagataga ccacacccat ggtgatggag accgcgacca gtcccaagat
     1561 cacaggaatt cccgaggcaa ccagcgctcc gaaggtgaat accaggatca ccaggacgat
     1621 ggggaatgac cgggactcgg cccaaaaagc gtcccgagcc gaggaatgaa tcatatcgta
     1681 ataagtagcg ccctccccgg tcacatagac agtcagccat ttggggattt tggtcccggc
     1741 gatgatccgg cgaatctccg ggactgcctg ctgggagttg aaaacattct caacatccag
     1801 ctctaccagg cccaggatac tgtggtgatc cttgcttgca agatcggcgc tattcgtatc
     1861 ataataggtg taaaccgtat taacctttaa cgaatccctc aagcccagtt ggactgcttc
     1921 caccgccgcc tggtactcgg gctcatcgta gtttcgggtc ttgctgtgaa aaaccaccac
     1981 cagctcgtaa ccgaagcggt tcccgaagtt attcttgagc agctccgtga cttcggctgc
     2041 ttctccgccc gggatcttgg tgtccccccg gagaatgccg tccaccctgg gggtgcaata
     2101 aattccgccc agcaccaaca ggcaccaggc tgccagcacc caccagcgcc tgcgtatcaa
     2161 ccagcggccg aaatgcgtca atattccatc cgacataacc ctacccccta gttgagtctc
     2221 tcaaacgtat catttgttgg ttccatcaaa agttgacctt cagtcccagg ttccattggg
     2281 ctttggtcgg gagcaggttg gcttcgctgt ccgttccgcc agttgccagc attcccttga
     2341 cgaacatctt cgtattctgc tgccaggtat actcaaacag cggctgaccc accaggctgc
     2401 cgtcgccggg gttgacaatc agtaacaatt cggtgttgat attgtccccg aaccccttgg
     2461 tggcccgaat gaagaaatga tcacgggtga tcccgccgtt gttggccaga ttaaccattg
     2521 tcgtttgtat ttctgtggat gccgcggcta gttttgctgt atcaagacca gcttggtata
     2581 acagattcgt actgtagttt ttaaactccg tagcatcctg atacgcttgg tcgttatggt
     2641 aatactcaag cgccaggtag taaccgcccg gcagattgta gtccgcacca accaagtagt
     2701 tgaggatatc cttcttatct gaacggcgat ttcccttcgc ctgatacagg gtctcgccat
     2761 gaatccccac tccggccagg ctgtaggcga aatcggcggt cgtcgccgcg ctgtcggttc
     2821 cgttttgggc taaacccagg gtcaggtcga ctccggcagg agaaaccttg aatcgtagcg
     2881 cccggccgga attctcccaa ctatcctgag gagtgattac tgcttgcgcc gcaaccggtc
     2941 ccagattcaa atctccccgg tatgccatga tcccgtcggg atcgtcgctg tcggtgaagg
     3001 cgttgcgcgg tatatccaaa atgtctgtgg ggttccaggc atacccggtg ccccaggcaa
     3061 gccgttgttt acccagacgc atactgaact ggtcgctggg ggtaaaatcg atgaaccccc
     3121 ggctgagact gaaactccct tgatcattgg gattttgaat ggtgtccctt aagtaactca
     3181 cccgcaaccg cactgtggtc tgatcattcg ggataagccg caggtcgtta cggagcttga
     3241 tactgtttcc cattgtcgga atgttatcca atgcgttagg aaccacggat aataaataat
     3301 aatcggtatt atgcgattgg ctgacaccgc cgacctccag caccccgcta taatcgatgg
     3361 tggttccggc ggcctcatcc gccagcacaa ccgtgaaact tcccaataag aaaagtaccg
     3421 ataaaatcaa cgccatgaat cgtttcacgt caaatcacct ctaaattcgt tatttattgt
     3481 tcgtgattca tgatagatga ctaagtaata atccctatct accctttccc tggtattgct
     3541 ggtcaaggtt gaggtaaact ccaaagtcca acaaacaagg cctgagaatc atacgaattc
     3601 cctgaaaacg gtgacaccgt tttctaaaga gttgtaatac aactctactt taaagggata
     3661 gggtagtgtt taggctacga agcctatcta cccttcccct taaagggtat tggctggaat
     3721 cgagggtcag taagctcggg ctacgaagtt taacaaatat tacctaaaat cagccaaaac
     3781 cccttagggg atagggtcgc ccttaggtct aatgtactta ctcttgtctc atgcctgttg
     3841 cctgttaccg cgcgccccgg ggggcgcgat tcattgccct ttctccagat actgcatgga
     3901 gaagtaacgt tcctgaatat cctcagtgaa acggatactg ttgatggtca ggatcgtctg
     3961 gtgccctttt tgctctaaat ccttgaaaat cattttggtg ggaacctggc gtccgtccat
     4021 ggtggtgatc tgcgtttggg tagcctcttt gaccagcctg ccgccgcccg
//
LOCUS       DASVMB010000357         7563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_559928,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000357 DASVMB010000000
VERSION     DASVMB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559928"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="plsX"
                     /locus_tag="VF531_10095"
     CDS             complement(<1..758)
                     /gene="plsX"
                     /locus_tag="VF531_10095"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7714364.1"
                     /translation="MKIAIDAMGGDYAPKEVVLGSVAACRELGADVILVGDEAAIKKE
                     LADASLTGLKLEIEPASEVIGMGEHPANAVKHKKDSSLVVANRLVKEGRADAVISAGN
                     SGAAMTASLLILGRISGVKRPALASPMPTKKGVSVLLDAGANADCDPENLLQFAMMGS
                     IYAELVLGLPHPRIGLLSIGEEETKGNKLIVESHQLLKESGLNFIGNIEGIDVHRGAC
                     EVIVCDGFVGNIVLKLSEGLATVLFGWVKEAITA"
     gene            complement(1028..1216)
                     /gene="rpmF"
                     /locus_tag="VF531_10100"
     CDS             complement(1028..1216)
                     /gene="rpmF"
                     /locus_tag="VF531_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7714365.1"
                     /translation="MANPKNRTSRTRTRLRRANWKLATPSIGTCPQCHEPKLPHQVCS
                     NCGYYNGREVISKATKNE"
     gene            complement(1197..1724)
                     /locus_tag="VF531_10105"
     CDS             complement(1197..1724)
                     /locus_tag="VF531_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7714366.1"
                     /translation="MKVNISSIRDVRGGALPISGTVDLQITRPELGFQFTGPVRVTGT
                     VTNTGEGFLIETLLTFTYEVNCGRCLERFAAEQSVELKEEFIPGYAKEDELAFGFQGD
                     EIDMTDCITEQVILALPMNFICQPECRGLCPSCGKNLNREACECVVKNINPQFAKLKS
                     LLSTEGGGTDGKSKK"
     gene            2002..2334
                     /locus_tag="VF531_10110"
     CDS             2002..2334
                     /locus_tag="VF531_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HEX7714367.1"
                     /translation="MGTGQKRNTPLVLEAHNALDSFKYEIANELNIDTSKIQGGYWGY
                     MMSRDTGAIGGNMVRRMIEAAERSLAEQTVADVKAGFQSGLSAAQSQLNQGVQSQFKM
                     NNPGNYTS"
     gene            2574..3563
                     /locus_tag="VF531_10115"
     CDS             2574..3563
                     /locus_tag="VF531_10115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7714368.1"
                     /translation="MTKSPMTDMGLTSGKTFHVDGAGISSTLSLILPFEWAGRTLESF
                     LRQQLHLSRNQIIALKHSQGIGINGRPVRIIYQIDGGERLELQFPGVNQSITPEPVPL
                     EICYEDQDLVIVNKPSGLVVHPVHQYPNGTLANGLIFHWQQLQEPASFHPLHRLDRLT
                     SGLVLVAKNPWVHQQLSRQLGNTFHRFYLAVCLGVPEPTSGRINQPLGRTGTGVKRIV
                     SGSGKPALTRYRVIRRTPTASLVAIRLLTGRTHQIRAHMSYLGFPLWGDSLYGVSDER
                     LTRPALHAVRIEFIHPRTRKRVRLNAPLPEDLTALLGELGMGLPESLITCPCR"
     gene            3704..5683
                     /gene="tkt"
                     /locus_tag="VF531_10120"
     CDS             3704..5683
                     /gene="tkt"
                     /locus_tag="VF531_10120"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7714369.1"
                     /translation="MNNPVLITANTIRGLAADMVEKAKSGHPGMPLGTADLAAVLFSE
                     FLRHDPLNPGWPDRDRFVLSAGHGSTLLYSLLYLTGYPLPFEELQRFRQWGSKTPGHP
                     EWGVTPGVETTTGPLGQGISNAVGMALAERMLAARFNRPGFEIVDHYTYAISGDGCLM
                     EGISSEASSLAGDLKLGKLIVFYDDNDISIEGSTDLAFKENVAARYKAYGWQVIEVNG
                     HDLEQIRGALKEAKTDSSRPTLIIAHTAIAKGSPLEGNAEAHGAPLGKENIAALKRNL
                     GLPEQEFFVPEEVKEYFASRRTAWQKDRQTWETNFDTWSKQFPELRREWDRIMARELP
                     ADLETALPRFEPGSASASRDAGGKTLNALAAKISELVGGSADLSPSTKTNLKDAPAVH
                     AGNYEGRNLHFGIREHAMGAILNGMSLHGGFRVYGSTFLVFSDYMRTPVRLAALMKQP
                     VVFVFTHDSFWVGEDGPTHEPVEQIESLRLIPGLRVIRPADGNETAWAWLEALRDTEH
                     PSALILTRQKLPTLSGATLNGFRHGGYTIKEADGGNPDLVILATGSEVALAVDAAAQL
                     GRIGKKARVVSVPCRELFVEQGPAYLKEILGTNLPTVAVEAGTGSGWYRLVGREGLVI
                     SIDHFGASAPAEKLAQEFGFTPEAVTQKIRTHFNW"
     gene            complement(5910..6491)
                     /locus_tag="VF531_10125"
     CDS             complement(5910..6491)
                     /locus_tag="VF531_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7714370.1"
                     /translation="MELGVDSRARGLIFDIDGTLADTMPTHFRSWQEVARRYGFAYPE
                     DWFYKNAGMSTERIVLFLNERFGYHLDPAEIARVKASIYLEIAENIGPIEPVARIARE
                     NFGKLPMALGTGECRTIAARNIRSAGLAPYFSIMVCAEDVPNPKPDPATFLKCATLMG
                     VEPQYCQVFEDGDPGLQAARSAGMIATDVRVFI"
     gene            complement(6858..7433)
                     /locus_tag="VF531_10130"
     CDS             complement(6858..7433)
                     /locus_tag="VF531_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7714371.1"
                     /translation="MQELIQKNRSYRRFYQDQAISEATLRGLVDLARLSASAANRQPL
                     KYILSWTPQRNELIFPCLAWAGYLKQWPGPAEGERPAAYIIVLGDTRISQSFGCDHGI
                     AAQSILLSAVEQGLGGCMLSAVNRPQLAQALHIPPHFEILLVLALGKPKETVVVEALP
                     PGGAVEYWRDDHQVHHVPKRDLAELLIELPE"
BASE COUNT         1875 a         2072 c         1822 g         1794 t
ORIGIN      
        1 ttggcggtga tcgcttcctt aacccaaccg aatagcaccg tggcaaggcc ttcggataat
       61 tttaaaacaa tgttgcctac aaaaccgtcg caaacgatga cctcgcaagc accccgatga
      121 acatcgatac cttcaatgtt accgatgaaa ttaagtccgc tctcctttaa aagctggtgc
      181 gattcaacaa tcaacttgtt ccctttggtc tcttcctcac cgatactcaa caatccaatt
      241 cgagggtgag gcaatcccag taccagttcg gcatagatcg aacccatcat cgcaaattgc
      301 agtaaattct cgggatcgca atcggcattg gccccggcgt ccagtaatac actgaccccc
      361 ttcttggtgg gcataggaga agccagagcc ggacgcttta cgccgctaat ccggcctaaa
      421 atgagtaatg aggctgtcat ggccgcgcca ctattaccag cggagatgac ggcatcagcc
      481 ctgccttctt tgaccaaacg gttcgcaacc accaaggaag agtctttttt gtgcttgacc
      541 gcatttgccg gatgctcacc cattccgatc acctcggaag cgggttctat ctccagcttc
      601 aatccggtta gggacgcatc ggccagttcc ttcttgatgg cggcttcgtc gcctacaaga
      661 ataacatctg cccctaactc cctacaagcg gcgacggatc ccaaaactac ttctttcggt
      721 gcataatctc cgcccattgc atcaatggca attttcaaat tataccaaca ccttttgtgt
      781 tgttagtttg gacattcaat cagtcgttta tgacccctgt caatcaaccg ggacggctgt
      841 cggggttatc gggagccctg taatccggac tcgaatcacc ccatacatca tatacggagc
      901 gactattttt tcagtatagc caaaagtata aaaaagtcaa ggaaatatat tgaatttttg
      961 ccaagacagt tcggagacct cgaaaaaatc aggccatcgc tctctaaaaa aataaggtca
     1021 tatgacctta ttcattcttg gtggccttgg aaataacttc ccggccgtta taataaccac
     1081 aattgctgca tacctgatgc ggcaatttcg gttcatgaca ctgcggacaa gtgccgatac
     1141 tcggagtcgc taatttccag ttggcgcgac gaagtctagt acgcgtacgg gaggtcctat
     1201 tttttggatt tgccatcggt tccacctcct tctgttgata acaaactttt aagcttggca
     1261 aattgagggt ttatattttt aacaacgcat tcgcaagctt ctcggtttag attcttaccg
     1321 caactggggc ataacccccg gcactccggt tgacagataa aattcatggg taacgccaga
     1381 attacctgtt cagtgatgca gtccgtcata tcaatctcgt cgccttggaa accgaaggcc
     1441 aactcatcct ctttggcata gccggggatg aattcttcct ttaactccac cgactgttcg
     1501 gcggcgaacc gttccaggca acgaccgcaa ttaacctcat acgtaaaggt caaaagggtt
     1561 tcaatcaaaa aaccctcccc ggtgttggta acggtacctg tcacgcggac cgggcccgtg
     1621 aattgaaatc ccaattccgg acgggtgatt tgaagatcca ccgttcccga gattgggagc
     1681 gcaccgccac gaacatcgcg gatcgaactg atattgactt tcactgcttt aaaaccccta
     1741 aataaaaatt cgccggcttc ctcgtgctac gcgcgtcgca tacgcacgtc cggcggaatt
     1801 gaagcgacat tcctcatcta aaggaaaatc accctgaata attatataag gtaatttttg
     1861 aaaagtcaat cgtttgccag tttaaaaaga aaggtcgtcc tacaggctcc ttccttatca
     1921 ggttttttcc gttactgcca aaaagaaagc aacatagctg gggtttgtaa gggtaagcta
     1981 accccggagg aggtgaacaa gatgggtact ggccaaaaac gaaatactcc gcttgtttta
     2041 gaggctcata atgctttaga cagctttaag tacgagatcg ccaacgaact gaatatcgat
     2101 acctcaaaaa tccaaggtgg ttactgggga tatatgatgt cccgggacac cggcgccatc
     2161 ggtggaaata tggtgcggcg gatgattgag gctgccgagc gttcactggc agaacagact
     2221 gtagctgatg taaaggcggg ttttcagtcg ggcttgagcg ccgctcaatc tcagcttaac
     2281 cagggtgttc aaagtcagtt caaaatgaac aaccccggca attacacgag ttaatcatta
     2341 cttacgagga gatatcttca aataggagat atctccttta tgtcggttcg ttttatcccg
     2401 cgtgcacgtg cgaaaccacc tcaaccaggt tttcattaaa aggccaaggc agcccttgcc
     2461 tcccccggac tggccgattc cactccaatt gacaaccagc attggttcgt ttacaatcaa
     2521 aacatgatcc acccaaaacg gatcgatttc acgcgcgtgc ggggaaggtt ttcatgacca
     2581 agtctcccat gacggatatg ggcttaacct ccggtaaaac ttttcatgtc gacggggcgg
     2641 gtatttcaag tacactatca ttaatcctgc ctttcgaatg ggccggcaga acgctcgagt
     2701 cattcctgcg gcaacaactt cacctttccc ggaaccagat cattgccctg aaacattctc
     2761 agggaatcgg aatcaacggt cgaccggtgc gaatcattta tcaaatcgat ggcggcgaaa
     2821 ggcttgaact tcaatttccc ggagttaatc agtctattac tccggagcct gtacctttgg
     2881 aaatctgcta tgaagatcaa gacctggtaa ttgtcaacaa gccgtccgga ttggtggtgc
     2941 atcccgttca ccaatatcca aacggtactc tcgccaatgg actgatcttt cactggcagc
     3001 agcttcagga acccgcctca ttccatcccc tgcaccgcct ggaccgttta acctcaggct
     3061 tagttttggt ggccaaaaac ccctgggttc atcagcaact ctcccgtcaa ctgggaaata
     3121 cttttcatcg gttctatcta gctgtatgcc tgggggtacc ggaaccgacc agcggacgga
     3181 ttaaccagcc cttgggccgt actggtacgg gagtgaaacg cattgtttcc ggatccggaa
     3241 aacccgcctt aacccgctac cgagtgattc ggcgaacccc gacagccagt ctggtggcga
     3301 tccggctact caccggtaga actcaccaga tccgggcgca tatgtcttat cttggtttcc
     3361 cgctatgggg ggattccctc tatggtgtct ccgatgaacg cttgacacgc ccggccttgc
     3421 atgcggtacg catagagttc atccatcccc gtacccggaa acgggttagg ctcaatgcgc
     3481 cactcccgga agatctgact gcacttctcg gagagctggg gatgggattg cctgaatcat
     3541 taattacctg tccatgcaga tgattaccgt tttaaccaaa tattcactac cgaaaaaagg
     3601 tatcctccgc atctctcggg aaataaacca aatagcaggg tccaaatccg gaccttaaga
     3661 ctatacaatt ctttgatcaa aaaacattgg ggggaagggt ttgatgaaca atccagttct
     3721 aatcacggcc aacaccatcc gcggactcgc cgcggatatg gttgaaaagg ccaaatcggg
     3781 gcaccccggg atgcccttgg gaaccgccga tctggccgcg gtacttttca gcgagttttt
     3841 gaggcacgat ccattgaatc ccgggtggcc cgaccgcgac cggttcgtgc tctctgccgg
     3901 ccatggttcg accctgttat actcattact atatttgacc ggatatccac tcccctttga
     3961 ggaactgcaa cgttttcggc aatggggttc caaaacgccc ggtcatcccg aatggggagt
     4021 aactccggga gttgaaacta ccaccggccc cctcgggcag ggaatctcaa acgcggtggg
     4081 aatggccctg gcagaacgga tgctggcggc tagatttaac cgtcccggtt tcgagatcgt
     4141 cgatcattat acatatgcca tctccggaga cggctgtctt atggaaggaa tcagctccga
     4201 ggcgtcctct cttgccggcg acctaaagct ggggaaactg atcgtctttt atgatgataa
     4261 cgatatcagc atcgaaggct ccactgactt ggcattcaaa gagaatgtcg ctgcgcgtta
     4321 caaggcatac ggctggcaag tgatcgaggt taacggccat gatctggaac agattcgcgg
     4381 ggcgctcaag gaagccaaaa ccgattcctc gcggccgacc ctgatcatcg ctcataccgc
     4441 gattgccaaa ggcagccctc ttgaaggaaa tgccgaagcc cacggtgctc ccctcggtaa
     4501 agagaatatc gcggctttaa aacgaaatct aggcctgccg gaacaggaat ttttcgttcc
     4561 cgaggaagtc aaagagtatt ttgcctcgcg gcggacagcg tggcaaaaag accgccaaac
     4621 ctgggaaaca aattttgaca cctggtccaa acagtttccc gaactacggc gcgaatggga
     4681 tcggatcatg gcccgggagc tcccggcgga tctggaaacg gctctgccgc ggtttgagcc
     4741 cggttcggcc agtgcttccc gggatgcagg cggtaaaacc ttaaatgctc tggctgcaaa
     4801 aatatccgaa ctggtaggcg gttcggccga tctatccccg tcaaccaaaa ccaatctgaa
     4861 agacgccccc gctgttcacg ccggcaatta tgaagggcgt aaccttcatt tcggcatccg
     4921 cgagcacgcc atgggcgcca tcttgaacgg catgtccttg cacggcgggt tccgggttta
     4981 cggttcgacc tttctggtct tttcggatta tatgaggact ccggtgcggc tagccgcact
     5041 gatgaagcaa ccggttgtct tcgtctttac ccatgactcc ttctgggtcg gtgaggacgg
     5101 ccccacccat gaaccggtgg aacagatcga atccctccgg ttgattccgg ggttgcgggt
     5161 catccgtccg gcagacggca acgaaacggc ctgggcttgg ctggaagccc tccgggatac
     5221 cgaacatccc agcgcgttga ttctgacccg ccaaaaactg ccgaccctct ccggagcaac
     5281 cttaaatggt ttccggcacg gcggttacac tatcaaggaa gccgacggcg gcaacccgga
     5341 tttggtcatt ttggccaccg gcagcgaggt ggctttggcc gtggatgccg cggcccaact
     5401 cggccggatc ggaaaaaagg ccagggtggt ttcggtaccc tgccgtgaac tctttgtgga
     5461 gcagggtccg gcatacctta aggagatact gggaaccaat cttcccaccg ttgccgtcga
     5521 agccggtacg ggaagcggtt ggtaccggct ggtcggcagg gaaggactgg tgatttccat
     5581 cgatcacttc ggcgccagcg cccctgcaga aaaactggct caggagttcg gattcacccc
     5641 ggaggcagtg acccaaaaga ttcggaccca ttttaactgg taaattaatg accgtaaggg
     5701 gtgtcccgaa agtagggaca ccccttttta atcagctata ctccattgtt taagggaaca
     5761 gcttgactcg tgagccttta catttggact tcgctgttta gcatgaacct attggctaag
     5821 atttttgccg gttcccttga agggaaaggg taataagctc aatcttaacg ggaatcagca
     5881 caactttaga gctttgttta accttcgatt caaataaaaa cccggacatc ggtggcgatc
     5941 atcccggcgc tgcgggcggc ctgtaacccg ggatcgccgt cttcaaaaac ctggcagtac
     6001 tgcggttcca ctcccatgag cgtggcgcat ttcaaaaaag tcgccgggtc cggtttggga
     6061 ttgggaacat cctcggcgca taccatgatc gaaaagtaag gtgccagccc cgcgctacga
     6121 atattcctcg cggcaatggt gcggcattct ccggtaccca aggccatcgg cagtttcccg
     6181 aagttttccc gggcgatccg ggccaccggt tcaatcggcc cgatattctc cgctatctcc
     6241 agatagattg aggccttgac acgggcgatc tccgccggat ccaggtgata accgaacctt
     6301 tcgttcagga agagcacgat ccgttcggtg gacatcccgg cgttcttata aaaccaatcc
     6361 tccggatacg caaagccata acgccgggct acctcctgcc aactgcgaaa atgggtcggc
     6421 atcgtatcgg cgagagtacc gtcgatatcg aagattaggc cgcgggccct tgagtctaca
     6481 cctaattcca taaaatgttc gctccatccg ttaaaaatct tacggatgaa gcttcgttac
     6541 ccaatcccgt gatacctgca cttttacaga aaattcacct atctctccct tggctctgat
     6601 gtcaatttaa ctaccgttat aacacgaaat tacaccaaaa gagaaatata tatcttaaat
     6661 accctttcgt atatttcgtg tgattaatta tttcgtgtgt ttcgtgcgat taaaaaagct
     6721 gtccttttta taaaccgtca tctctctcca gaccggccta tcgggtgaat agaaacgaat
     6781 cacgaagaac acgaaatttc acgaaaaaag agaaccccct aaacagaagg tcctcttgta
     6841 aaacaattaa ctcgcgacta ttccggaagc tcgatgagca gttccgccag gtcccgcttg
     6901 gggacatgat gcacttgatg atcatcgcgc caatactcga ctgcccctcc gggcggcaac
     6961 gcctccacca ccacggtctc cttgggctta cccaaggcca ggaccaacag gatctcaaaa
     7021 tgcggcggga tatgtaatgc ttgcgccagt tgcggccggt taaccgcact caacatacat
     7081 ccgcccaaac cttgttccac tgcgctcaat aagatgctct gggcggcaat accatggtcg
     7141 caaccgaacg attggctaat ccgggtatcc cctaacacga tgatgtaggc tgcaggccgc
     7201 tccccctccg ccggaccggg ccactgcttt aagtatcccg cccaggccag gcagggaaag
     7261 atcaattcgt tccgctgcgg cgtccaggat aatatgtact tcaacggctg gcggttagct
     7321 gcggaagcag agagccttgc caaatcgaca agtcctcgta aagtagcttc actaatcgct
     7381 tgatcctggt aaaatcggcg atagctccgg tttttctgaa taagttcctg aatcgtggca
     7441 actccccctt acggctgata taaattcgat taatcctata ttaactcgtt tccataacca
     7501 tgtaaatata taccatggcg attaagactc cccggttgta tccgccacag ccccctcatc
     7561 ctc
//
LOCUS       DASVMB010000358        13160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_189970,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000358 DASVMB010000000
VERSION     DASVMB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189970"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..998
                     /locus_tag="VF531_10135"
     CDS             276..998
                     /locus_tag="VF531_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7714372.1"
                     /translation="MDDPVFQHPAHCEVGGFITIQPGDTLYSLARRYNITVQAIVAAN
                     PGINPNDLPVGELICIPSPIPGQPFPERCPNGITYTIQSGDTFYSIARRFNVSLDALL
                     AANPGVNPDSLQIGERICVPTLPTPTVTCPGPTYTIRPGDTLFSLARTLGFSVQAILA
                     ANPGINPNALQVGQILCLPTGGTVSCPNGTIYQVQPGDTLYGIARRFDITLDRILRAN
                     PGLSTNSIIFPGQPLCIPIREI"
     gene            complement(1105..1920)
                     /locus_tag="VF531_10140"
     CDS             complement(1105..1920)
                     /locus_tag="VF531_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714373.1"
                     /translation="MLILFVMLILVIIPLITLIIALFMKGDKVRDACRMAGFATGAFV
                     WNAYTYWLAWPVVSAVLLILAGVGILRLLVAGRKILFQGMMVAAITGLFFFLAWQGGL
                     QAILNTAKVQNPVYVNSAGPTGSALIVYHPGGSSFQSKIINGFASGLAQEGWRIKIVT
                     TGSKITTDFKEYDLVVFSSPTYEWLPSRRIITYIKDISSLKGIPTAVIVSGVRSTEHS
                     QPALEQAVKDKGAKFVGSYVIWTSGSKKIYGINDPVEAMRREGSKLVRAMETK"
     gene            complement(1957..2754)
                     /locus_tag="VF531_10145"
     CDS             complement(1957..2754)
                     /locus_tag="VF531_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061234.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7714374.1"
                     /translation="MKTAIYYFTGTGNSLEVARDIHQKLSDSHFVSITALLDMEAIIP
                     QSQIVGMVFPVHDWNLPCVVRNFVEKLELDSVTYIFAVANCNFLAGGTLDHLDRLLKE
                     KGHSLTAGFVIRMPGTYLPLYGPNPERMQQQKFRRKDRDIDKIIRIVSTNRPYKIEYK
                     GIFVDKWLSPLFRQDSAEIRRSDANFHVDGDKCTGCGICTKVCAFKSIELVDGKPQWQ
                     HRCQRCFACIHLCPRMAIQYGNHTEKKKRYKNPNVTLQEIISSNKQM"
     gene            complement(2761..3384)
                     /locus_tag="VF531_10150"
     CDS             complement(2761..3384)
                     /locus_tag="VF531_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7714375.1"
                     /translation="MQYKKDEVKERIDKAALAVFSSKGYGNTKIADIAAKANVSVGNI
                     YLYYKGKDDIFYSIVPESFWESLKSLLFNKIGMFREDLGNQIGSIMINRDFIKYLIEN
                     RERMLILFTGSDGTRYENIKEQLVNFLIATIKEHYFEEYRQQLEEERKGEVIIKKVYQ
                     NLLDLYFGLLKEEETIEGLQSDLKMINEYHLFGITGLLKIKNQGKLE"
     gene            complement(3620..4978)
                     /locus_tag="VF531_10155"
     CDS             complement(3620..4978)
                     /locus_tag="VF531_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7714376.1"
                     /translation="MKFIVFIALNIISGTLFVLFHKQKSKIAFYYAGYISAFSLGLLA
                     ITLDPDPEHYIMPFALPHLLARICSVVSYRMSPFFLLNASISVVGIYSNNPKKRTLLS
                     LITFLPVFIGFVIDGMNPRQSFIFSYPDYLPNFWWMFSSWGLFYTLLSNILLYYAVII
                     ENVPQVKRQKMIIAFLALPTLFIAWNVYISPVLGKHDFSNLNATFGVFIFITIIIAVA
                     RTGFIGLKLSFEHDYMDRSIKTLYSGASLINHAVRNELQLINIAAETIKTNSPESMKY
                     IRVIENASHHLEDLVRRFSEKTKPITLYIEEIDINQLVDEILSSMEKMFIQKSINVTK
                     QCLENSPTVKCDRVHVEEVLLNVLYNSIEAMPQGGSITLAIYIKGRTMNIDVTDSGIG
                     MSKYDMKHILEPFYSTKKDEKNLGLGLTYCFAVMKQHHGSLHITSGEDSGTTVTLSLP
                     KR"
     gene            complement(5149..5715)
                     /locus_tag="VF531_10160"
     CDS             complement(5149..5715)
                     /locus_tag="VF531_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7714377.1"
                     /translation="MIVNETLKIIKERRSIRSYQEGQISEEELQAVLEAGLYAPNAGD
                     QAWHFTVVQNKELLDRLNLTAKETARQMNIPGLRELGNDEKFNCLYGAPTLIIVSGNE
                     RAPMPLDADCAAATQNLLLAAESIGLGTCWIFFVLLAFRSSQGSALRQELKIPEGYKP
                     YYSAVFGYKNAETVKVPDRKPNLITFIR"
     gene            complement(5815..7473)
                     /locus_tag="VF531_10165"
     CDS             complement(5815..7473)
                     /locus_tag="VF531_10165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7714378.1"
                     /translation="MEHLKVEFHDLLERFGKAQSFELQDQVISDINRLRNEFETMVTL
                     VHIRHSIDTTDEHYDRENDYFDETTPIYEGLITDYYRVLVSSPYRTALEEKWGRQVFR
                     LAELKLKTFAPEIVEDLKQENKLSSQYTKLRASAQIMFEGEVRNLPQMSPFLQSPDRN
                     TRKKAQEAYTGFFLQHEAEFDQIYDGLVKLRNKIARKLGYKNFVELGYARMGRSDYDA
                     TMARGYREQVLEAIVPLATKLRHRQAERLNLEKLKYYDEPLEFLTGNAVPKGTSDQIL
                     ANGKQMYSELSPQTGEFFNFMTEHGLLDLVTRKGKAGGGYCTYIPNYRSPFIFSNFNG
                     TSDDVDVLTHEAGHAFQVYLSRDYQLPEYIWPTLEACEIHSMSMEFLAWPWMRLFFEE
                     DEIKYKFAHLSGALLFIPYGVTVDEFQHWVYENPEATPAERKRIWREIERKYLPHRDY
                     EDNDLLNRGGYWFRQGHIFNDPFYYIDYTLAQVCAFQFWIRSQENRTTAWEDYLRLCK
                     AGGSMAFLELVKLAGLKNPFEPGCVRRVVGPITAWLDEVADKKL"
     gene            complement(7669..8973)
                     /locus_tag="VF531_10170"
     CDS             complement(7669..8973)
                     /locus_tag="VF531_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7714379.1"
                     /translation="MKDNKRIINRNLILLLMGRMVSDTGTGIQMVVMPLYIIDAGGSA
                     ATVGLFSFLSLVPALLVYPFAGVLGDRINRKIIMVATDLASAIVIVGLAFCSYSNRMS
                     LTLLLVVQVIVSLLYGLFDPATKGMLPRLVAGEELAQANSTVASLRILSGLLSPVVGA
                     ALYANLGITVLFLINGISFLLSGSSEMLIRYQHAQRESTDGIRGFVTDLSEGIGFITA
                     HKVIRKLCLFFLVIYALVQPIFTVVLPLFFRTRLNYSDTQYGYLQMAMILGALLGSIL
                     VGLLFGKEKEANKSLVLGCSLLMGAMLAFSVVLLPHSLSVLGKDTVIYLALLAGVLCL
                     LSAAIMFINVPVQTFIQRETPNEYMSRMFSIVGMITKGGMPFGALIYGIILNRVEVYW
                     AVLTATIVMTGISVVFLALFLQNPRVGAANLNEKAEMKREKQ"
     gene            complement(9085..11724)
                     /gene="ppsA"
                     /locus_tag="VF531_10175"
     CDS             complement(9085..11724)
                     /gene="ppsA"
                     /locus_tag="VF531_10175"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX7714380.1"
                     /translation="MNSYVLGFQEIDRTKLMNVGGKGANLGELSRIEGIRVPEGFCVT
                     TEAYCRNVAQNKDCNALLDQLSLLKAEERERISEISGKIRKIIEEIPIAKEIVEEITR
                     FHSKLGENTACAVRSSATAEDLPNASFAGQQDTYLNIIGKDAILKHISKCWASLFTDR
                     AVIYRIQNGFDHRKVYLSVVIQRMVFPQTAGIMFTADPVTSNRKVLSIDASFGLGEAL
                     VSGLVNADIYKVRDGRIIDKKISTKKQAIVALAKGGTEKRGIEPGQQNIQTLTDNEIL
                     ELERIGRKIEVYFGRPQDIEWCLYENTFYVVQSRPITTLYPAPDNRDGKNRVYFSFGH
                     RQMMTEAMKPLGITFFRDFFKLISGSAMCEIGGRLYIDTSRELRSPLMSKTFIKGLDT
                     VDVLMQNAFYNLMKRQDFIKSLSRGKAAMIGGTVWLTWGTQTLKNYYQNDPMVVKNLI
                     AHHEALMHDREQRIPKLSGDELFEFILKDFEEIKKAVFAGYGAVFAGAWAAYWINKNI
                     QKWLGEKNAADILAQSVSNNVTSEMGLELLDVADVVRQYPAVLEYFQHPGDETFFSDL
                     AKLEGGKSVSDSIRAYLKKYGMRCSAEIDITRTRWNEKPTILIPMILSNIKAFGPDAH
                     RMKFEQGLREAKQKEEDILRRLEQLPGGKRKAGKTKKAISVLRNFAGYREYNKYLIVW
                     YLWIARQALLKEADGLVRKNVIREREDVYYLTFEEFREVVKTNRLASSHQSVREMITG
                     RREEYQVFEKLTPPRVITSEGEVISGEYDTGKIPKGAFAVVPVSSGIIEGRARVILKM
                     EDANIEEGDILVTTFTDPSWTPVFVSIKGLVTEVGGMMTHGAVVAREYGLPAVVSVEN
                     ATKLIRDGQRIRVNGTEGYVEIL"
     gene            complement(11737..12327)
                     /locus_tag="VF531_10180"
     CDS             complement(11737..12327)
                     /locus_tag="VF531_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7714381.1"
                     /translation="MPKETFFNLSEEKKQRIFDAAVQEFATRRFSEASLNQIVKAAQI
                     PWGSFYQYFSGKEDLYLYMLDRISGEKREIARHSETLDREADFFEICIQTIKATFEWS
                     RHNPQYIQIAILMEIDNSEFIAKIRSSLSESFGKLVERDKERGLIKPDVDSDLVGDMI
                     YTLIWKQFYLAGSDEELFYKRLTEGINIIKEGIAKS"
     gene            complement(12495..>13160)
                     /locus_tag="VF531_10185"
     CDS             complement(12495..>13160)
                     /locus_tag="VF531_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7714382.1"
                     /translation="RMVLNHTSGLSNDPWGIDRKVYFTPGDHFSYSGGGFRYLQTVLE
                     TVSGVPFREYMERELLKPLAMSRSSFVYRDDFVGNIAYGHQNGSVLPLRSTPANGAGS
                     LLSTPTDLARFGMELCDPTLLPAQAVDSMLTPAVRIDEVYSWGLGIGLCRTSAGDIAW
                     QWGDNVIYQNLIMILPKEHSGVVIMTNSSNGLQIAPLVAVQLLNCWIENPIDPSELKP
                     FSF"
BASE COUNT         3575 a         3318 c         2604 g         3663 t
ORIGIN      
        1 aacgaaaagc tgcttaactt ttgaagttcc cgatgacaaa ttgcgaactt tactggacag
       61 aaccgctgat acttccgaag aaatggcagc tggaaagctc ggaaaagata attgcaaaga
      121 gtgaaggttg acatgtgaaa caaaaaaggg gctgacacac caaacagccc cttaagaata
      181 tccgagtatt ttgcacccac ggcatttctt aagtccccat cccctggact ttttcatata
      241 ctatagcgat tttcaatgaa agaagggaat cgcctatgga cgatccagta ttccaacatc
      301 cagcgcactg tgaagtcggt ggttttataa ccattcagcc cggtgatacc ttatacagcc
      361 tggcccgacg ttataatatt acagtccagg ccattgtcgc cgccaatcca ggtattaacc
      421 ctaatgattt accagtcggc gaattaatct gtattcccag tccaataccc ggtcagcctt
      481 ttcccgaacg ttgcccaaat ggaataacct acacaattca gtccggtgat actttttact
      541 ccattgcccg acgattcaat gtctcattgg atgcgctttt agccgccaat cccggagtta
      601 atcccgatag ccttcaaatc ggagaacgaa tctgcgttcc aaccttgccc accccgaccg
      661 tcacctgtcc cggacctacg tataccattc gtccgggaga tactttattt agccttgccc
      721 gaactcttgg ttttagcgtc caggcaattt tggccgccaa tcccggaatc aacccgaatg
      781 ctctccaagt tggccaaatt ctatgcttgc cgaccggagg taccgtttcc tgtccaaatg
      841 gaaccattta ccaggtccaa ccgggggata ctctttatgg aatcgcccga cgatttgata
      901 taacgctgga tagaatacta agagctaatc ccggactttc aaccaattcg atcattttcc
      961 ccggtcaacc gttatgtata cccatccggg agatatagga agaagatgtt ccagcagaac
     1021 ccgatcccga gccccatgaa tgaacttcgt tactcccggc cgaaggaatc aacatatcgg
     1081 tgacggtcta ttcatcatgg aagtttactt tgtctccatg gcccgtacca gtttactccc
     1141 ttctcgccgc atcgcctcaa cgggatcgtt tatgccgtaa atttttttcg agcccgatgt
     1201 ccaaatcaca tatgagccaa caaatttcgc ccctttgtct tttacggctt gctctaaagc
     1261 cggttgtgaa tgttcggttg agcgtactcc ggaaacaata actgcggttg ggatcccttt
     1321 caagctggaa atatctttga tgtatgtgat gatgcgccgg gatggcagcc actcataagt
     1381 cggggaacta aaaaccacca gatcatattc tttgaaatct gttgttatct ttgaacctgt
     1441 tgtaacaatc tttatgcgcc agccttcctg agcaagacca ctggcaaacc cattgattat
     1501 tttagattga aaactcgatc cgccaggatg atatacaatc agcgccgatc cagttggacc
     1561 ggccgagttc acatataccg gattctgcac ttttgcagta tttagtatgg cttgaagtcc
     1621 cccttgccaa gccaggaaga aaaatagacc ggtgattgca gccaccatca tcccttggaa
     1681 taaaattttc ctgccggcta ccaacaatcg cagtatacca acgccggcca gtatcagaag
     1741 caccgccgat acaacgggcc atgccaacca atacgtatat gcgttccaaa caaacgcccc
     1801 agttgcaaac cccgccatcc tacaagcatc ccgtacttta tctcccttca tgaacagggc
     1861 tataattaat gtaattaggg gaatgatcac caaaatcaac atcacaaaca aaatcaacat
     1921 ctgaattttc tcctcctcga cagcaatgcc tttaatttac atctgtttat tggatgaaat
     1981 aatctcctga agagtcacat tcgggttttt ataccttttt ttcttttccg tatggttacc
     2041 atattgaatc gccatccggg ggcataggtg gatacaggcg aagcaccgtt ggcaacggtg
     2101 ttgccattgg ggtttaccgt caaccaattc aatactctta aaagcgcata cctttgtaca
     2161 aatgccacac cctgtgcatt tatctccatc cacatgaaag ttggcgtcac ttcggcggat
     2221 ttccgcggaa tcttgtctaa ataacggtga caaccacttg tcaacgaata ttcctttata
     2281 ctcgatctta tatgggcgat tggtgctgac aattcgaata attttatcaa tatcccggtc
     2341 ctttcggcgg aacttttgct gttgcattct ttcaggatta gggccgtata acggtaggta
     2401 agtgccgggc attcttataa caaaaccggc agttaaagaa tgcccttttt cttttaatag
     2461 cctatcaaga tgatcgaggg tacccccggc taaaaaatta cagttggcta ccgcaaaaat
     2521 ataggtaaca ctatcaagtt ccagtttttc aacaaaattt cggaccacgc acggtaaatt
     2581 ccagtcgtga acaggaaaaa ccattcccac aatttggctt tgcgggataa ttgcctccat
     2641 atccaacaga gcagtaatcg atacaaaatg cgaatcactt aatttctggt gaatatcccg
     2701 cgccacttcc agactattac ccgtaccagt aaaataatat attgcggttt tcaaaaactt
     2761 tcactccaat ttaccttgat tctttatctt caataatccg gtaatgccaa ataaatgata
     2821 ctcattaatc atttttagat cactctgtaa accctcaatg gtttcttctt ctttcaagag
     2881 accgaaatat agatccagta aattttgata taccttctta ataatcacct ccccttttct
     2941 ttcctcttct agctgttgcc ggtattcttc aaaataatgc tctttaattg ttgcaatgag
     3001 gaaattgaca agttgctctt ttatattttc atatctcgtg ccatcactgc ctgtgaagag
     3061 aatcagcatt cgttcccggt tttcaattaa atacttaatg aaatctcgat taatcataat
     3121 tgaccctatc tgattaccca ggtcctcccg gaacattcca attttgttga agagtaaact
     3181 tttcaatgat tcccaaaaac tttccggaac aatcgaatag aaaatatcat cttttccctt
     3241 gtaatacaga taaatatttc ctacagaaac attcgccttt gccgcaatat cggcaatttt
     3301 tgtgtttcca tatcccttgc tggaaaacac cgccaacgcg gccttatcga ttctctcttt
     3361 tacctcatcc tttttatatt gcacttgttg tctccgcgta aaaatgaatt tgtgattcgt
     3421 ttttattgta aactattttt ggaaaagtgt caacaacatt tacattattt gtcatgcgtg
     3481 cttatccaac ctatatattt gttccaaaca atctgaagta aagttctctc tttaaagtgg
     3541 aaggaataaa aaaagagaaa aattccgttc tcccggattt ttcccttaac tcttctctct
     3601 tttcactttt gattcaacct caccttttcg gtaaagaaag cgtaacggtt gttccactat
     3661 cctcgccgct tgtaatatgg agtgaaccat gatgctgctt catgactgcg aagcaatatg
     3721 taagtcctaa tccaagattc ttttcatcct tttttgttga ataaaatggt tccaaaatat
     3781 gtttcatatc atattttgac atgccgattc cgctatcggt aacatcaata ttcatagttc
     3841 gccccttgat atagattgcc agagtgattg agccgccttg gggcatggct tcaattgaat
     3901 tatacaaaac atttaaaaga acttcttcaa cgtggacccg atcacatttc acggttggtg
     3961 aattttccaa acactgtttg gtaacgttga tgcttttctg gataaacatc ttttccattg
     4021 aactcaaaat ctcatccacc aattgattaa tatctatctc ttcaatatag agtgtaattg
     4081 gtttcgtttt ttcggaaaac cgtctcacca agtcctccaa gtgatgggag gcattttcga
     4141 ttaccctgat atatttcatg gattccgggg aattcgtttt gatggtttca gctgcgatat
     4201 tgattaattg caattcattt ctgacggcat gattaattaa ggaagcaccc gaataaagcg
     4261 ttttaatcga tcgatccata taatcatgct cgaaacttag ttttaggcca atgaatccgg
     4321 tacgggcaac agcaattatg attgttatga aaatgaacac tccaaaggtt gcgtttaaat
     4381 tggaaaaatc gtgcttgcct agaaccgggg agatataaac attccaagca ataaacaatg
     4441 tcggcaatgc taaaaatgcg ataatcattt tttgccgttt gacttgcggt acgttttcaa
     4501 taatcaccgc ataataaagt aaaatattac tcaataacgt ataaaataag ccccatgatg
     4561 agaacatcca ccaaaagtta ggtaaataat cgggataaga gaagataaaa ctttggcgag
     4621 gattcattcc atcaattaca aaacctatga ataccggaag aaatgtgatc aaacttaaca
     4681 aagttctctt tttgggattg ttgctataaa tgcctacaac tgaaattgac gcattgagca
     4741 gaaaaaaagg actcatacga taagatacta ccgaacatat ccgggccaag agatggggga
     4801 gagcaaacgg catgatatag tgttcgggat cggggtccaa ggtgatggcc aataacccca
     4861 aggaaaaagc cgatatgtaa ccagcataat aaaaggcaat cttcgatttt tgtttatgaa
     4921 ataaaacgaa aagagtgcct gaaattatgt tcagtgctat gaaaacaata aatttcaatt
     4981 atactcaccc gaaatataat ctccaaatca aacttaccag ttcgctggaa acccatctgg
     5041 agatcggcgt ttctacttct ttcgattcga caatttggaa gaaatacctt taacagttac
     5101 tggatcgggg ctcgacattt tcccttttca catttgtttt tcaatttttt atctgataaa
     5161 tgtaatgagg ttgggtttcc tgtccggtac cttgacagtc tcggcattct tatatccaaa
     5221 tacagccgaa taataaggtt tataaccctc ggggatttta agttcttgtc gtaatgcgga
     5281 cccttgagac gaacgaaagg ccaacaaaac aaagaaaatc cagcatgtcc ccaacccgat
     5341 ggactctgcg gcaagcagca ggttttgagt ggcggcggcg caatccgcgt caagaggcat
     5401 tggggcccgt tcattcccgg agacgatgat cagtgtcggt gcaccatata aacagttgaa
     5461 tttttcatca ttccccagct cccttaaacc tggaatattc atttgcctgg ctgtttcttt
     5521 ggctgtaaga tttaatcgat ccaatagttc cttgttttga accactgtaa aatgccacgc
     5581 ctgatccccg gcgttcggag catacagccc cgcctccagt actgcctgta attcttcctc
     5641 gctgatctgc ccttcttgat aacttctgat gcttctgcgt tctttaatga ttttcaaagt
     5701 ctcattgacg atcatcaaat cttcccccga tggttttgaa aaggtaaagt aaaaatgatc
     5761 aacataagtt taaccatttt tcatttttcc cttttctctt ttccttttca atttctagag
     5821 tttcttatcc gctacttcat ccaaccacgc cgtgatcggt ccgactaccc tccggacaca
     5881 tcccggttca aaaggatttt ttaaacccgc cagtttcacc aattccagga aagccatgct
     5941 tccacccgcc ttacaaagac gtaaataatc ctcccatgcc gtagtacggt tttcctggga
     6001 acggatccaa aattggaagg cgcaaacttg ggctaaagta taatcaatat agtaaaacgg
     6061 atcattaaaa atatgacctt gccggaacca gtatccacca cggttcagca gatcgttgtc
     6121 ctcgtaatcg cggtgaggta aatactttcg ctcgatctcc cgccagattc gcttgcgttc
     6181 cgcgggcgtc gcctccgggt tttcgtaaac ccaatgttgg aactcatcga cggtcacccc
     6241 atacgggatg aacagcaaag cgccgcttaa gtgggcaaat ttatacttga tttcatcctc
     6301 ctcaaagaaa agccgcatcc acggccaagc caaaaactcc atactcatgg aatggatttc
     6361 acaagcctcc agcgtaggcc agatatattc cggcagttga taatcccggc ttaaatacac
     6421 ttgaaaagca tgacctgcct catgagtgag aacatccacg tcatccgaag ttccattgaa
     6481 gttggaaaag atgaagggcg accggtagtt gggaatatag gtgcaataac cgccgcccgc
     6541 ctttcctttg cgggtgacta aatcgagcag cccatgttca gtcatgaaat taaaaaactc
     6601 gccggtctgg ggagaaagct cactgtacat ctgcttgcca tttgcaagga tctgatctga
     6661 agttcccttt ggaactgcat taccggtaag aaactccagg ggttcatcgt agtatttcaa
     6721 tttctccaaa ttcaaacgtt ccgcctgtct atgccggagc ttcgtcgcca gcggcactat
     6781 ggcttcaagc acctgttccc gataacccct ggccattgtg gcgtcgtagt ccgatcgccc
     6841 catacgggca taaccgagtt cgacaaaatt cttatatcct agtttacggg ctattttgtt
     6901 ccgcagcttt accaaaccat cgtatatctg atcaaactcc gcttcgtgct gaagaaaaaa
     6961 gccggtataa gcctcttgcg ccttcttccg agtattccta tccggtgatt gcagaaaggg
     7021 ggacatttgg ggaagatttc tgacttcacc ttcaaacatg atctgggccg aagctcttaa
     7081 ttttgtatat tggctcgaca gtttgttctc ttgcttaaga tcttccacga tctccggagc
     7141 gaaagtcttc agtttcaact cagccaaccg gaaaacctgc cggccccatt tttcctccaa
     7201 ggcggtacgg tacggcgaac tgaccaaaac ccggtagtaa tcggtgatta acccttcata
     7261 gatgggagtt gtctcatcga agtaatcatt ttcccggtca taatgctcat cggtggtgtc
     7321 gatggagtgc cggatatgga ccagagttac catggtttca aactcgttcc ggagacgatt
     7381 tatgtcggag ataacttggt cttgcaactc aaaggattgg gcttttccaa accgttccaa
     7441 aagatcgtga aattcaacct tcaaatgctc catatccggc cggttatagt gatattcttt
     7501 aaaagtttgc acggtaatac cccctcatat aaaagtgaaa aatgtaaaat gaaaactttc
     7561 tttttggctg atactgcttg gtaataactt tcaaggttga tatcgtcatt cgccactcca
     7621 tcattcttgc ctgccggttt ccatttcact cctattgctt tctccgtttt attgtttttc
     7681 ccttttcatt tctgcctttt cgtttaggtt tgccgcgccg acccgggggt tttgaagaaa
     7741 caaggccaaa aaaacgactg aaatccctgt catcactatg gtagctgtca gtacggccca
     7801 atatacctca accctgttaa ggataattcc gtaaattaat gcaccaaatg gcatgccgcc
     7861 cttggtgatc attccaacga ttgaaaacat ccgggacata tattcattgg gcgtttcccg
     7921 ttggataaag gtctgcaccg gaacattgat aaacatgata gcggcgctta acaggcaaag
     7981 cactcctgcc agcaaagcaa ggtatatcac cgtgtctttc cccaggacgg ataaactatg
     8041 cggcaagagc acaaccgaga acgcgagcat tgctcccatg agcaggctac aacccaatac
     8101 cagcgattta ttggcttcct tctctttgcc gaataacaga cctaccagaa tacttccgag
     8161 taacgctccc aatatcattg ccatttgcag atatccatac tgtgtgtcgg agtaattaag
     8221 gcgggttctg aaaaaaagcg gcaatacaac ggtaaatata ggttgtacca aggcataaat
     8281 caccaggaaa aaaaggcata atttgcgaat tactttatga gccgtaataa agccgatacc
     8341 ctcggacaaa tccgtaacaa acccccgtat tccatccgtt gactcccgtt gggcatgttg
     8401 atacctgatc agcatttcac tgcttcctga cagtaaaaat gagatcccgt taatcaagaa
     8461 caacaccgta atgcccagat ttgcatacag ggcggcaccg acgaccgggc taagcagtcc
     8521 tgacaatatc cgcaacgatg ccacagtgga gttggcctgg gccagttctt ccccggccac
     8581 caaccggggc agcatccctt tggtggccgg atcaaacaaa ccataaagca gagatacaat
     8641 tacctgtaca actaggagca aagttaagct cattctattt gaatatgaac aaaatgccaa
     8701 ccccactatg actatggcac tggccaagtc agtcgccacc ataatgatct tgcggttgat
     8761 cctgtccccc aacactccgg caaagggata gaccaggagt gccggcacta aagacaggaa
     8821 ggaaaacagt cccacggtag ccgcagagcc gccggcatcg ataatataca agggcattac
     8881 caccatctgg ataccggttc ccgtatcgga taccatcctc cccatcaaga gcaggataag
     8941 attacgattg atgattctct tattatcctt caccaaagcg gcacctcaat tcttttccac
     9001 ctaagaaagc gaccctgact ggggtcgctt tcagataact tcatatcatc cgtcccaaaa
     9061 cagccgaaga gcatatcttc cctgttacag gatttccaca tatccttccg ttccatttac
     9121 cctgattctc tgaccatcgc ggatcagctt ggtggcgttt tccacgctta caaccgcagg
     9181 aagaccgtat tcccttgcaa caacggcacc gtgagtcatc atcccgccca cttcagtcac
     9241 caggccttta atcgatacaa ataccggtgt ccagcttgga tcggtgaatg tagtgactaa
     9301 aatatcgcct tcctcgatat tggcatcctc catttttaag ataacccgcg ccctgccctc
     9361 aataattccc gatgaaacgg gcacgaccgc gaaagcccct ttggggattt tgccggtgtc
     9421 atattcgccg gatatgacct caccttcgga agttattacc cggggaggcg tcagcttttc
     9481 aaacacctga tattcctctc tccgtccggt tatcatctca cgcacgcttt gatgcgagga
     9541 agccaagcgg tttgtcttga caacctctct gaattcctca aaggtcagat aatagacatc
     9601 ctccctctcc cgaatcacgt tctttcgcac caaaccatcg gcttccttta gcaaggcttg
     9661 cctggcaatc cagagatacc aaacaatgag atacttgttg tattcccggt aacccgcaaa
     9721 attacgcaaa acgctgattg ccttcttggt ctttccagcc ttgcgcttgc cgccgggcaa
     9781 ttgctccagg cgtctcagga tatcttcttc cttttgcttc gcttcccgaa ggccttgttc
     9841 aaatttcatg cgatgggcgt ccggtccaaa ggccttgata ttgctgagaa tcatagggat
     9901 gagtatcgtc ggcttctcgt tccaccgcgt tctggtaata tcaatttccg cggaacaacg
     9961 catgccatat tttttaagat aagcccggat cgaatcgctt acggattttc caccttccaa
    10021 tttggccaga tcactaaaga aggtctcgtc accgggatgt tgaaaatatt caagcaccgc
    10081 cggatactgc ctgacgacgt ccgcaacatc caaaagttca agccccattt cggatgtgac
    10141 attattggat accgattggg ccaggatatc ggctgcattc ttttcaccca gccatttctg
    10201 aatgttttta ttgatccagt atgcggccca agctcccgca aaaaccgctc cgtaaccggc
    10261 aaagacagcc ttttttatct cttcaaaatc cttcaagatg aactcaaaca gctcatctcc
    10321 ggataatttc ggaatccttt gctcccgatc atgcatcagc gcctcatgat gagcgattag
    10381 gtttttaaca accatcgggt cgttttgata ataatttttt agggtctggg tcccccaggt
    10441 caaccagacc gtgcccccga tcatcgccgc ctttccccgg gacagcgatt tgataaaatc
    10501 ctgccgtttc atcaagttat aaaaagcatt ttgcatcaaa acatcgacgg tatcaagacc
    10561 tttgataaaa gttttgctca ttaacggtga tctgagttcg cgggaggtat cgatatataa
    10621 ccttccgcca atctcacaca tcgccgagcc ggaaatcagc ttgaaaaaat cccgaaagaa
    10681 ggttatacct aacggtttca tagcctccgt catcatctgg cgatgaccaa aggaaaaata
    10741 cacccggttt tttccgtccc gattatccgg tgcagggtat aaagtcgtga tgggacgact
    10801 ctgaaccaca taaaatgtgt tttcatagag acaccattcg atgtcctgag ggcggccaaa
    10861 atatacctca atctttctgc cgatacgctc aagttccaaa atctcattgt ccgtcaacgt
    10921 ttgaatattt tgctgccccg gttcgatccc ccgtttttca gtgccgccct ttgctaatgc
    10981 gacgatggcc tgtttcttgg tagatatttt tttatcgata atcctgccgt cccgcacttt
    11041 gtagatatcc gcattcacca gtccggagac gagcgcctcg cccagcccaa agctggcatc
    11101 gatggacagc accttcctgt tggaggtgac gggatcagcc gtgaacataa ttccggctgt
    11161 ctgggggaaa accatccgct ggatgactac ggacagataa accttacggt ggtcgaaacc
    11221 gttttggatg cggtagatta cggcccggtc cgtaaacagc gacgcccagc acttgctgat
    11281 atgcttcagg atggcgtctt ttccgataat attcagatag gtatcctgct ggcctgcaaa
    11341 tgaggcattc ggcaggtctt ccgccgtggc gctggagcgt acggcacagg cagtgttttc
    11401 gcccagcttt gaatgaaagc gagtgatctc ttccactatc tccttggcaa tgggtatctc
    11461 ttcaatgatc ttgcgaatct ttccgctgat ttcgctgatt cgttctctct cttccgcctt
    11521 taaaagggat aattgatcaa gtaaggcgtt gcaatccttg ttttgcgcaa catttctaca
    11581 atacgcttcg gtggtgacac aaaagccttc cggtacccgt atcccctcaa tcctggacaa
    11641 ttcccccagg ttagcccctt tgccacccac attcatcagc ttcgtcctat cgatctcctg
    11701 aaaaccaagg acatatgagt tcatgtcggt acccccctac gatttagcaa ttccttcctt
    11761 gataatattg atgccctccg tgagtctctt ataaaacagc tcctcatccg atccggcgag
    11821 ataaaactgc ttccaaatca gtgtgtaaat catgtctccc acaaggtcgg agtctacatc
    11881 cggcttgata aggccgcgct ctttatcacg ctccaccaat ttcccaaaac tttcacttaa
    11941 agatgatcga atcttggcaa tgaattcact attatcaatt tccatcagaa tggcgatctg
    12001 gatatattgc ggattatggc ggctccattc aaaggtggct ttgatggttt ggatacagat
    12061 ttcaaaaaag tcggcctccc gatccaaagt ttctgaatgt cgtgcaattt cccgcttctc
    12121 ccctgaaatc cggtcaagca tataaagata aagatcctct ttgccgctaa aatactgata
    12181 aaagcttccc cagggtattt gggcggcttt tacaatctgg ttgagggatg cctcgctgaa
    12241 acgccgggtt gcaaattcct gtaccgcagc gtcaaagatt ctttgttttt tttcctcact
    12301 caaattaaaa aaagtttcct tcggcacact gccacctccc tggcaacatg ataacacaaa
    12361 ttcccgaata tgagaagcct ctcacatgag aaacttctca ctttttaact ttcaccctcc
    12421 gatttcaagt ttaaagcaaa aacccgcaga tattctgcgg gtccagattt ttttgaaatt
    12481 gattacggta aaaattaaaa cgaaaaaggt tttagttcac tcggatcaat tggattctcg
    12541 atccaacaat tcagcagttg caccgcaacc agaggggcga tctgcaggcc gttcgagctg
    12601 ttggtcataa tcaccacccc actatgttct ttaggaagga tcattatcag attttgataa
    12661 atgacattat ccccccattg ccaggcgatg tctccggctg aagtcctgca taacccgatc
    12721 cccagccccc atgagtaaac ctcatcaatc ctgaccgcag gagtcaacat actgtcgaca
    12781 gcctgagccg ggagcagggt ggggtcgcag agttccattc caaaccgggc cagatcggtc
    12841 ggagtggata aaagactgcc tgcgccattg gccggggtgc tccgtagcgg gagaacactt
    12901 ccgttttggt gcccgtaagc tatattaccg acgaaatcat cccgatatac aaaggaactg
    12961 cgggacatcg ccagtggttt caagagttcc ctctccatat actcacgaaa aggaactcca
    13021 ctcaccgttt ccaaaacggt ttgcaaatat cgaaaaccgc cgcccgaata tgaaaaatgg
    13081 tcgcccgggg tgaaatagac ttttcgatcg attccccaag gatcgtttga cagtcccgag
    13141 gtatggttca gtaccatgcg
//
LOCUS       DASVMB010000359         5274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_267980,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000359 DASVMB010000000
VERSION     DASVMB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267980"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VF531_10190"
     CDS             complement(<1..679)
                     /locus_tag="VF531_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7714383.1"
                     /translation="MRNTVKGRKERVSFGKIDEILDMPNLIEIQQRSYQWFLDEGLRE
                     MFRDISPIQDFTGNLVLEFIDYALGEPKYPVDECKERDVTFAAPLRVRARLINKETGE
                     VKEQEVFMGDFPLMTEKGTFIINGAERVIVSQLVRSPGVYFGRTQDTSGKYLYSSSVI
                     PNRGAWLEFEFDANDALYVRIDRTRKVPVTILLKALGLGNNYQLTEVFEGKESIKNTL
                     ERDNTQNE"
     gene            complement(1106..1477)
                     /gene="rplL"
                     /locus_tag="VF531_10195"
     CDS             complement(1106..1477)
                     /gene="rplL"
                     /locus_tag="VF531_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7714384.1"
                     /translation="MSKVTDVLEIVKGMSVLELNELVKAFETEFGVSAAAPVAVAAAP
                     AGAAPATEEKTEFNVILANAGGQKIQVIKVVREATGLGLKEAKDLVDGAPKAVKEGMP
                     KKDAEELKKKLEEAGATVELK"
     gene            complement(1539..2063)
                     /gene="rplJ"
                     /locus_tag="VF531_10200"
     CDS             complement(1539..2063)
                     /gene="rplJ"
                     /locus_tag="VF531_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7714385.1"
                     /translation="MSIRSEKEATVSEVQQKFAKSRTVVFADYRGLNVQEVTELRKKL
                     REAGVEYRVIKNTLTSRAAKEANVEGLDPLLSGPTAIAFGYNDVVTPAKILYNFAKDH
                     KQLTLKGGILEGQVIDFEAVKSLASLPSREALLTQLAGLMQAPMRGLVTVLSGPLRNV
                     TYALEAVRKQKAEA"
     misc_feature    complement(2113..2301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2356..3069)
                     /gene="rplA"
                     /locus_tag="VF531_10205"
     CDS             complement(2356..3069)
                     /gene="rplA"
                     /locus_tag="VF531_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7714386.1"
                     /translation="MAKHGKRYLESVKLIDQSKQYEPTEALSLLKNMPKGKFDETVEL
                     SIRLGVDPRQSDQQIRGAVVLPHGTGRSMKVLVFAKGEKAKEAEEAGADRVGAEELID
                     EIQKGWLDFDVAIATPDMMGAVGKLGKVLGPKGLMPNPKTGTVTMDVIKAVKDSKAGK
                     VEYRVDKAGIVHVPIGKVSFEPEKLVRNFRTLMDAIVKAKPSGAKGTYIKSVVVSSTM
                     GPGIKIAPQLASQSVAAEH"
     gene            complement(3154..3579)
                     /gene="rplK"
                     /locus_tag="VF531_10210"
     CDS             complement(3154..3579)
                     /gene="rplK"
                     /locus_tag="VF531_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7714387.1"
                     /translation="MAKKITGFIKLQVPAGKANPAPPVGPALGQHGVNIMEFCKNFNE
                     RTANQAGMIIPVVITVYEDRSFTFICKTPPVPVLIKKAINLEKGSGTPNKNKVGTISK
                     AKVREIAELKMADLNAASVEAAMSMVAGTARSMGVVVEK"
     gene            complement(3730..4257)
                     /gene="nusG"
                     /locus_tag="VF531_10215"
     CDS             complement(3730..4257)
                     /gene="nusG"
                     /locus_tag="VF531_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX7714388.1"
                     /translation="MEEKNWYVIHTYSGYENKVKANLERRAESMGMEDKIFRILVPTE
                     EEMELRDGKRKVTKKKIFPGYVIVEMILTDDSWYVVRNTPGVTGFVGTGSKPIPLQEK
                     ETKLILHQMGIEEPHTKIDFEVGETVKVIRGPFENFSGIIEDINPEKGKIKVMVSMFG
                     RETPVELDYHQIEKL"
     gene            complement(4314..4538)
                     /gene="secE"
                     /locus_tag="VF531_10220"
     CDS             complement(4314..4538)
                     /gene="secE"
                     /locus_tag="VF531_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX7714389.1"
                     /translation="MLPAKKEQKVSPGEGLRRFFRNVMAELKKVNWPTRKELTNYTIV
                     VVATVLVVALIIFAWDSLLTFIFKAMGFYR"
     gene            complement(4559..4708)
                     /gene="rpmG"
                     /locus_tag="VF531_10225"
     CDS             complement(4559..4708)
                     /gene="rpmG"
                     /locus_tag="VF531_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7714390.1"
                     /translation="MREGITLACSECKNRNYRTNKNKKNNPERLEIMKYCKFCRKETN
                     HKEAR"
     gene            complement(5031..>5274)
                     /gene="tuf"
                     /locus_tag="VF531_10230"
     CDS             complement(5031..>5274)
                     /gene="tuf"
                     /locus_tag="VF531_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780383.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7714391.1"
                     /translation="EEGGRHTPFFNGYRPQFYFRTTDVTGVAHLPEGVEMVMPGDNIK
                     MTIELITPVAMEEKLRFAIREGGHTVGSGVVSKVIE"
BASE COUNT         1296 a         1392 c         1104 g         1482 t
ORIGIN      
        1 cttcgttttg ggtgttatcc cgttcgagag tgtttttgat actctctttt ccctcaaata
       61 cttcggtgag ctgataatta ttccccaggc cgagcgcctt gaggagaatc gtcaccggaa
      121 ccttacgggt ccggtcgatc cggacataca aggcatcatt cgcatcaaat tcaaactcca
      181 gccaggcgcc gcggttcggt ataacactgg acgaataaag atatttaccg gatgtatcct
      241 gggttcttcc gaaatatacg cctggcgaac gaaccaattg gctgacgatg acccgttcgg
      301 ctccgttgat gatgaaagtg cctttctcgg tcatcagagg gaaatccccc ataaacactt
      361 cttgttcctt cacttctccg gtttccttat tgatcaaacg cgcccttacc cgtaacgggg
      421 cagcgaaagt aacatcccgt tccttgcact catcaaccgg atatttcggt tcccccaatg
      481 cgtaatcaat gaactccagc accaggttac cggtgaaatc ttgaatcggt gagatatccc
      541 gaaacatctc ccgaagaccc tcatcgagaa accattgata tgaacgttgt tgaatctcga
      601 tcaagttcgg catgtccaag atttcatcga ttttgccgaa actaacgcgt tcctttcgcc
      661 ctttcaccgt attgcgcaag gtaattcacc tcttgtagaa ttatgtccac tttaggaact
      721 aacttccaga agaaacagat aaaagcaagg taacattgtt agcctcgctt acaacaaatc
      781 tcatttctaa cccatatttt ccgtgagatt caacttgatc aagatttccg gagatcaact
      841 cgcgttcata taatttaacc tgtttgtaac aggattatgc gcaactttga agtagttgct
      901 ttgaagtgat ttgaaaatga aatgattcaa acaactgatc ggcagatccg gccattttaa
      961 aacattcaac cagtctccaa cttcagcaga atataataat atcacagggt tttctaggtg
     1021 tcaagcaaat tttaggttaa acgaaaagag gagcataaat cggctcctct ttaatactgg
     1081 gacgtacaaa accggtatcg accagttatt tcagctcgac tgtagcaccg gcctcttcca
     1141 atttcttctt gagttcctcg gcatccttct tgggcatacc ttccttgacg gctttcggcg
     1201 cgccgtcaac aaggtccttg gcttctttga ggccgagacc ggtagcctca cgaactactt
     1261 tgataacttg aatcttctga ccgccggcat tcgccaggat tacattaaac tcggtctttt
     1321 cctcggtagc cggagcagca ccggccggag cagcggccac agccaccgga gcggcggcgg
     1381 atacgccgaa ctcggtttca aacgccttta ccagttcatt cagttccaaa acggacattc
     1441 ctttaacgat ctcaagtaca tcagttactt tagacattga tgaatagcct ccttaattaa
     1501 taaaataatc atttgggaaa acctgttggc ctaacggatt acgcttcggc tttttgttta
     1561 cggacagctt caagcgcata ggttacattc cgtaacggac cggaaagtac cgtcaccaaa
     1621 ccgcgcatcg gagcctgcat caaaccggcc aattgagtca gtaaagcctc acgggacggc
     1681 aatgacgcta acgacttaac agcctcgaaa tcaatgacct gtccctccag gatgcctcct
     1741 ttgagggtta attgcttgtg gtccttggca aagttgtaaa gaatcttggc cggagttacc
     1801 acatcattgt aaccaaaggc aatcgccgtc gggccgctta aaagcggatc aagtccttcg
     1861 acattggctt ccttcgcggc tctactggtt aacgtgtttt tgatgacgcg gtattcaacg
     1921 ccggcttccc gaagcttctt ccgaagctcg gttacttcct gtacgttgag tccacggtaa
     1981 tcggcgaaga caacagttct ggacttggca aatttctgtt gaacctcact gactgtggcc
     2041 tctttctcgg aacgaatcga catcttagca cctcccttga tcggaattct tcgtgggcgc
     2101 attaattagc gcctacaaaa acgaaaaacc tccatagatt tggaggtttt gttgtccata
     2161 caaaaatgta ccgaaaatac tcggcgggta atagacccat tacattccag gacatcaagc
     2221 ctcggtaggc gttcaaccag tccggtcgat cattatctcc atatggagac ctactgtcta
     2281 tggcaaaaat aacgcatgtt tcaagtcgca tcatcacaca tgatgatttt cgaaaaaaca
     2341 accgtgtgcg tgcgtttaat gctcggccgc aaccgactgt gatgcaagtt gaggcgcgat
     2401 cttgattcca gggcccatag tggaagagac cactacgctt ttaatatacg tacctttggc
     2461 gcccgagggt ttcgctttaa caatcgcgtc catcaacgtc cggaagttac ggaccaattt
     2521 ctccggttca aaagaaacct tgccgatggg tacatgaacg ataccggctt tatctacccg
     2581 gtattcaacc ttaccggctt tactgtcttt cacggctttg attacatcca tggttacagt
     2641 accggttttg gggttcggca tcaatccttt gggacctaat accttaccta atttaccgac
     2701 tgcgcccatc atatccggag tagcgatggc cacatcaaaa tccagccagc ctttttggat
     2761 ctcgtcaatc agctcttcag caccgacgcg gtctgctccg gcttcctccg cctccttggc
     2821 cttttcacct ttggcgaaga ctaaaacctt catggaacgt ccggttccat gaggcaatac
     2881 aactgcaccg cggatctgtt gatcagattg gcgtggatcg actcccaacc ggatggataa
     2941 ctcgactgtc tcgtcgaatt tccctttcgg catattcttt aatagcgata aagcttcggt
     3001 cggctcatat tgttttgatt ggtcgatcaa ttttacagat tcaagataac gcttgccatg
     3061 ttttgccatt tttatttcct cccttggtgg tttaacggaa attcttccgg cttaagtttc
     3121 ccggtctagc atttcctccc acaactggtg tcgttatttc tcaacgacaa ctcccatact
     3181 gcgcgcagtg ccggccacca tgctcatagc cgcctcaacg ctggctgcat tcaggtcagc
     3241 cattttcaac tcggcaattt cacggacttt tgccttgctg attgtgccca ccttattctt
     3301 gttcggagtt cccgaaccct tctccagatt gatggctttc tttattaata ccggaaccgg
     3361 gggagtttta caaatgaatg tgaaggaacg gtcctcatat accgtgataa ccacgggaat
     3421 gatcatcccc gcttgatttg cggtccgctc attgaagttc ttgcagaact ccatgatgtt
     3481 gacgccgtgt tgtccgagtg ccggaccaac tggcggtgca ggattggctt taccggctgg
     3541 aacttgaagt ttgataaagc ctgtaatctt tttagccatt gcctcgcgcg aaaaaaacgc
     3601 gcgccacctc ccttaatata atgtggtcat atcgggtaaa tattcctccg aagagctgtt
     3661 tggcggctct tcagagggtt aagtgcattg atacgcggtt tggcgtatca caccctccca
     3721 ctcaaacggt taaagctttt ctatctgatg gtaatcgagc tcaacagggg tttcccgtcc
     3781 aaacatcgaa accatcacct taatcttacc cttttcagga ttaatatctt caataatccc
     3841 ggagaaattt tcaaatgggc cgcggatgac tttaacggtt tcgcccacct caaaatcgat
     3901 cttggtatgg ggttcttcta tccccatttg atgcaagatt aacttggttt ccttctcctg
     3961 taacggaatg ggtttggaac cggtacccac aaaacccgtg acaccgggcg tgttccggac
     4021 aacataccag gaatcgtcgg tcaagatcat ctctacaatc acatagcccg gaaagatctt
     4081 ttttttggtt acttttcgct tcccatcgcg cagttccatc tcttcctcgg tgggcaccag
     4141 gatccggaag atcttgtctt ccatccccat ggactccgcc ctgcgttcca ggttggcctt
     4201 aactttattt tcatatccgg aataggtatg gatcacatac caattctttt cttccatcca
     4261 aacttctcct cacccgtaac tttaaaaaac ctgtcaccag caacaggtgg caattaacgg
     4321 taaaatccca ttgctttaaa gatgaaagtt agtagcgaat cccaagcaaa tatgatcaaa
     4381 gctacgacca aaacggtggc aaccactacg atggtatagt tggtcaactc tttgcgggtc
     4441 ggccagttga ccttctttaa ttcggccatg acgtttcgga aaaagcggcg caggccttca
     4501 cccggcgata ctttttgctc tttcttggcg ggtagcatct tcctcacccc ttaatcgtct
     4561 atcttgcctc tttatggttg gtttctttac ggcagaattt gcagtatttc atgatctcaa
     4621 gtcgttcggg attgtttttc ttatttttgt tcgtacggta gttgcgattt ttacattccg
     4681 agcaagccag ggtgatccct tcacgcatag ctgccacctc cttacatcgt aagcagtaac
     4741 tctaatttga aaaaaggcga aaaaaataaa gacccattgg atcagaagtc acttttaaaa
     4801 tttaccacaa ttatatcgaa ctgtcaaggg tatttctcgc tccgccgcac ttggacgaag
     4861 tccgccttat caagaccgct gtttttttag cgaccgctgc cttttagcgg ccattgcctt
     4921 ccgaccaagc ataaacccaa ttctcaatat tcatttccta tatatcctcg ggagcaaacg
     4981 aaaaggctac cctaattaga gtagccttgg gtgtctatta aaccgttctc ttactcgatg
     5041 accttgctca caactccgga acccacggta tggccacctt cgcgaatagc gaaccgcagc
     5101 ttctcttcca tggccaccgg ggtaatcaac tcgatcgtca tcttgatgtt gtcgcccggc
     5161 atcaccatct ccactccctc aggcagatgc gccactccgg taacatcggt cgtccggaag
     5221 tagaattgcg gccggtatcc attgaagaac ggagtatgac gtccgccttc ttct
//
LOCUS       DASVMB010000360         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_634394,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000360 DASVMB010000000
VERSION     DASVMB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634394"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..277)
                     /gene="atpE"
                     /locus_tag="VF531_10235"
     CDS             complement(44..277)
                     /gene="atpE"
                     /locus_tag="VF531_10235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX7714392.1"
                     /translation="MTLGMLIAVVVGVICVGASFAAAFADAKVISTALEGMARQPEVQ
                     NRLFLSMLIGVGLVEVVPIISIVISFILVGRIG"
     gene            complement(314..973)
                     /gene="atpB"
                     /locus_tag="VF531_10240"
     CDS             complement(314..973)
                     /gene="atpB"
                     /locus_tag="VF531_10240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7714393.1"
                     /translation="MEISHNIISLFGLQVDLWIAIMTWIVMGILIVISWLATRHLSWI
                     PKGMQNLLELAVEYIENLIRGSMGEAGIKYTYFFGSLFLFILVANMLGLVPGFSSPTR
                     DLSVTVCLAVLWVFWMEYIGIRENGLKAHLKHYIEPLPPFIVVHLLDLFTRPLTLSMR
                     LFANIFAGEILLEKLTEIFRLIVPSVWIVMSIMIGAIQALIFTILTVSYTGISVSHED
                     N"
     gene            complement(957..1331)
                     /locus_tag="VF531_10245"
     CDS             complement(957..1331)
                     /locus_tag="VF531_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714394.1"
                     /translation="MRRYLYVPIFIILTVAMLFLARLEGYVAAAILGTDAIVLDLYWQ
                     WLRFILVRLGKTSHMLWGILGGFVLRIACLLAFIKLSSLFFKAFSSYFYVFAILLLTI
                     PLWSLIVAYKFKQESNRHGDLS"
     gene            complement(1328..1552)
                     /locus_tag="VF531_10250"
     CDS             complement(1328..1552)
                     /locus_tag="VF531_10250"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7714395.1"
                     /translation="MKPKDSNKEFWRILGLYGFLSLNLGLTIAGGYFLGNFLEKYYRL
                     QNAALIGVLVGLFLGLYEMFRIAYKAGQKK"
     gene            complement(1690..2859)
                     /gene="wecB"
                     /locus_tag="VF531_10255"
     CDS             complement(1690..2859)
                     /gene="wecB"
                     /locus_tag="VF531_10255"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7714396.1"
                     /translation="MNNIIRLTIQNPKAIKICLIFGTRPEAIKMAPVFMALSKQPEFH
                     VKTVVTAQHREMLDQVLELFGWRPDDDLNIMTPGQTLAEVTSRVLERLTPILQTERPD
                     LILVHGDTATTFAASLAAFYLKIPVAHIEAGLRTGDPWQPYPEEMNRILTGHIAELHF
                     APTAQARANLLRENIQSSRILVTGNTVIDALQYCKGLLPKDQNDSPDRWILVTAHRRE
                     NWGQPLENMIIAFKRILGAYPEVHFLIPMHRNPVVREIFRHGLGNEPRVRLVEPFDYR
                     EMVAAMSQAYLVITDSGGIQEEAPSLGKPVLVLRDKTERPEAVVAGTVKLVGTDPERI
                     WAAAEILLSDSKAYQRMAQALNPYGDGLAAKRILAGLRFSCGLSNQLPEEFTCLQ"
     gene            complement(3130..3759)
                     /gene="upp"
                     /locus_tag="VF531_10260"
     CDS             complement(3130..3759)
                     /gene="upp"
                     /locus_tag="VF531_10260"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX7714397.1"
                     /translation="MSNVFVINHPLIQHKLSIIRDQNTGAKEFRELVEEIAMLMAYEV
                     TRNFPLEEIEVETPIATARCQVVSGKKVAVIPILRAGLGMTGGVLKLIPTAKVGHIGV
                     YRDPETLQPIEYYCKLPPDIEERELILVDPMLATGGSAAAGIQFLKQRGATQIRLMCL
                     IAAPEGIQNVQAKHPDVDIYVAAVDDRLNSHGYIVPGLGDAGDRLFGTK"
     gene            complement(3792..4508)
                     /locus_tag="VF531_10265"
     CDS             complement(3792..4508)
                     /locus_tag="VF531_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEX7714398.1"
                     /translation="MIKQVLFVCTGNTCRSSMAEALLRKMLTEDLAERAATIRVVSAG
                     TGAVTGEQAAQNAIEVMAGEGIDLRSHRSRRLTPELVSAADLVLTMTIDQKRTVLDIV
                     PQAKGKVFTLSEFATGMKEIEALMEQAEKIRALLEEKKRKYLEHEGPKLEELRRRNLE
                     LSRQMRALDEELRRIEQKLDQEIYPEKKELEAIQAKFTSLEISDPYGQNIEAYRVCAS
                     EIKQKLKTVVKRIEESLDDV"
BASE COUNT         1240 a         1195 c         1054 g         1139 t
ORIGIN      
        1 tcagcccgga gtcaacaata cttgtatccg cttgctggga gcgttagcca atccggccta
       61 ccaaaatgaa ggagataaca atcgagatga tcggcaccac ttcgaccaaa ccgacaccga
      121 tcaacatgga aaggaaaagc cggttttgaa cctccggttg acgggccatt ccttccaaag
      181 ccgtggagat taccttggca tccgcaaatg ccgccgcaaa gcttgcacct acacaaatca
      241 caccaactac aactgcgatt aacatcccta aagtcattac ctacaccccc gctaaagttt
      301 agttttggta ttttcaatta tcctcatgcg atacggagat acccgtatag gatacggtaa
      361 ggattgtaaa gattaacgcc tgaatcgcgc cgatcatgat gctcatgact atccatacgc
      421 tgggaacgat tagccggaag atctcggtga gcttttcaag cagaatctca ccggcgaaga
      481 tgttggcgaa cagccgcatc gacagcgtga gcgggcgggt aaaaagatcc aacagatgga
      541 cgacgatgaa tggcggcagg ggttcgatat aatgcttgag atgagcctta agaccgttct
      601 cccgaatccc gatgtactcc atccaaaaca cccataaaac cgcgagacag accgttacac
      661 taagatccct ggtcggggag gaaaaaccgg ggactaaacc gagcatgttg gcgaccagaa
      721 taaaaaggaa aagcgagcca aagaagtagg tatatttgat ccccgcctca cccatgctgc
      781 cccggatcag gttttctata tattcaaccg ccaattccaa cagattttgc atccctttgg
      841 gaatccaact caggtggcgt gtggcgagcc aagagattac gattagaatg cccatgacga
      901 tccaggtcat gatagcgatc cataaatcga cctgtagccc gaagaggctg atgatgttat
      961 gagagatctc catgtcgatt gctctcctgt ttgaatttgt aagctacgat caaactccag
     1021 agcggtatgg tcaagagtaa aatggcgaaa acatagaaat aggagctgaa ggccttgaag
     1081 aataaggagg aaagtttaat aaaggctaaa agacaggcga ttcgtagcac aaacccgccc
     1141 aaaattcccc atagcatatg gcttgtctta ccaagacgga ccaaaataaa acgtaaccat
     1201 tgccagtaga gatcgaggac gattgcgtcc gttcctagaa tagcagcggc aacataccct
     1261 tccagacggg ccaggaagag cattgccacg gtgaggatga taaaaatcgg aacgtacaag
     1321 tagcgtctca ttttttctgc cctgctttgt aagcaatccg aaacatctca taaagcccta
     1381 ggaataaacc gacgagtacg ccaatcaaag cggcgttttg aagccggtag tatttttcta
     1441 aaaaatttcc taaaaaatac cctcctgcaa tggtcaggcc aagattcaag cttaaaaagc
     1501 cgtacagccc caaaatgcgc cagaattctt tattgctatc tttcggcttc atgaagaacc
     1561 tcctggccga aaccttggca ataatcaccg ggattgctcg attcgccagt tcatttacat
     1621 ttcttgatcg cgcctatatc atatataaca ccgaaagaag atccttaaca gccttttact
     1681 gtaaaagaat tattgcaaac atgtaaattc ttcgggcaac tgattactga gaccacagct
     1741 aaaacggagc ccggccagaa ttcgcttggc ggccaaacca tcgccgtaag gattcagcgc
     1801 ttgagccatc cgttggtaag ccttagaatc gctcaagagt atctctgccg ctgcccaaat
     1861 cctctccgga tccgtgccca ccagtttaac cgtaccggcg acaaccgctt cgggccgttc
     1921 ggttttatcg cgtaatacca gcaccggttt accgagcgat ggagcttctt cctgaattcc
     1981 accggaatcg gtgatgacca gatatgcctg gctcatcgcc gccaccatct cccgataatc
     2041 gaaaggttca accagcctga cccggggttc gtttcccagc ccgtgccgga aaatctctcg
     2101 gactaccgga tttcggtgca tcggaattaa aaaatgaacc tccgggtagg ccccaagaat
     2161 ccgtttgaat gcgataatca tattttcaag gggctggccc caattttcac ggcgatgggc
     2221 agtgaccaaa atccagcggt ccggggaatc attttggtct ttaggtaaaa gtcccttgca
     2281 gtattgcaag gcatcgatca ctgtatttcc agtaaccagg atcctggagg actgaatgtt
     2341 ctcccgcagc aaattggcgc gggcttgggc ggttggcgca aaatgcagtt ctgcaatatg
     2401 tccggtcaag atgcggttca tttcttccgg gtaaggttgc catgggtctc cggtccgtaa
     2461 tccagcttca atatgcgcca ccggtatctt caggtaaaaa gccgccagac tcgcggcaaa
     2521 agtcgttgcg gtatcaccgt gtacaagtat aagatccggc cgttcggtct ggaggatcgg
     2581 ggttaaccgt tccaaaactc tggaggttac ttccgccaag gtttgacccg gggtcatgat
     2641 attgagatca tcatccgggc gccacccgaa aagttccaga acttgatcga gcatttcacg
     2701 gtgttgtgcc gtaaccactg ttttcacgtg aaattccggt tgcttactta atgccatgaa
     2761 taccggcgcc attttaattg cttcgggccg ggttccaaag atgaggcaaa tcttgatcgc
     2821 ttttggattt tgaatcgtca agcggatgat attattcaag aattcccccc cattatattt
     2881 tagaatactg ctggaatggt tgaatcacaa atcgaatttt ctagcggata ttgattaata
     2941 tcgccaatat ggttgatttt tggaaaataa gtccgctagg gattcccttg atcccttttt
     3001 tactggtaac taaccgcaaa acgagataaa aaaaccggct gaaagccggt ttttcactgg
     3061 gatatttgaa cttggttgaa cagatctgcc atgaactggc ccgcaattta ccagattcag
     3121 tcaacccttc tatttggtgc cgaagagtcg atcgccggca tctcccaagc cgggaacgat
     3181 gtatccatgg gagttgagcc gatcatccac tgcagccacg tatatatcca cgtccggatg
     3241 tttcgcctgc acgttctgaa taccctccgg ggctgcgatc aggcacatca aacggatttg
     3301 agtggcgccg cgttgcttca gaaactggat tccggcagca gccgaaccgc cggtcgccag
     3361 catcggatcg accaatatca attcacgttc ttcaatgtct ggcggcagtt tacaataata
     3421 ctcgataggc tggagggtct ccggatcacg gtagactccg atatgtccca ccttggcggt
     3481 agggataagt ttgagaaccc caccggtcat tcccagaccc gcccgcaaaa tagggattac
     3541 tgcaaccttc tttcccgaaa ccacctgaca acgggcggta gcaatcggcg tttccacctc
     3601 gatctcctcc agcgggaaat tacgagtgac ctcatacgcc atgagcatcg ctatctcttc
     3661 cactaactcc cggaactctt tagccccggt attctgatcc ctaatgatgg agagcttatg
     3721 ttggatcaga ggatgattaa tgacaaacac atttgacacg aaaatcgctc ctttaagttt
     3781 atcaagtagt gctatacgtc atccagggat tcctcaatac gtttgacaac tgttttcagc
     3841 ttttgcttaa tctccgaagc gcagacccgg taagcctcta tattctggcc atagggatcg
     3901 gagatttcaa gggaagtgaa cttagcctga atcgcctcaa gttctttttt ctcgggatat
     3961 atctcctgat cgagtttctg ttcgatccgg cgaagttcct catctaatgc cctcatttgg
     4021 cgactgagtt caagattgcg gcggcgtaat tcctctagct ttggcccctc atgctccaag
     4081 tactttcgct ttttttcttc aagcaatgcc cggatctttt cggcctgttc cattagggct
     4141 tcaatctctt tcataccggt tgcaaactca ctcaatgtaa acaccttgcc cttggcctga
     4201 ggaacgatgt ccaacacggt ccgtttttga tcaatcgtca tcgtaagcac cagatccgcc
     4261 gcagacacca attcgggagt taaacgccgg gaacgatggg agcgtaaatc aataccctcc
     4321 cccgccatca cttcaatcgc gttttgagca gcctgttctc cggtgactgc cccggtaccc
     4381 gcggaaacaa cccggattgt agcagcccgc tccgctaagt cttcggtaag cattttacgg
     4441 agtaatgcct ccgccatact actgcggcag gtatttccgg tgcaaacgaa taaaacctgt
     4501 ttaatcatga aaccgttgat cctcccaaat tcctagaaag cagtatccaa aaaaatagat
     4561 gagggcatga tcaagtttgg gcatgaactc gtgatgctgt aaaacccatt catatcataa
     4621 accatgtc
//
LOCUS       DASVMB010000361         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_268576,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000361 DASVMB010000000
VERSION     DASVMB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268576"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF531_10270"
     CDS             <1..634
                     /locus_tag="VF531_10270"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEX7714399.1"
                     /translation="DDSFLSLLKAAGMENNWKMAPVGGEMRPEIQVSMWKYTPPKGLE
                     DYNACVDLTHASWLLDQGVFDQPPTGLKWCRAMEAARRLGYELFVPKSFLFSTIKKDT
                     TIKLGVQIQNTGIAPFYYDWTVQLGIRNSSGEVIKTYDTNWRISKIMPGPKPMDFEWT
                     IDRPGLEVGSYTMVMRVVNPLKNGKKLMFADVEQGENGWLDLGGFQVTKG"
     gene            705..1685
                     /locus_tag="VF531_10275"
     CDS             705..1685
                     /locus_tag="VF531_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714400.1"
                     /translation="MNSISLADISIKGELAVRAGLNYARLEGQWYRPDEVFQADKHGW
                     PADWEGRIILALTLLAQSTHRTPAYLDRIIALLPEHLNRKGYFGPIVPEGKFDEQHLS
                     GHSWILRGLIEHYYWKKDEHVKQILETMLHNYILTAKGYYAQYPIDPATRFQDPVWIL
                     SKLQTKTKHHAETSDAGCAFILIDGVTQAYQLFRWPELKEFADEMIQRFMEIDLMKLH
                     IQTHATLSSLRGILRLYELENDAAYLQKAQKVFDYYKDEAWTEAYGNYNWFGAPRWTE
                     PCAIIDSFIVATSLWKYTGNPQYLEDAHRIYYNALCHGYRGNGSFGTDRC"
     gene            1755..2423
                     /locus_tag="VF531_10280"
     CDS             1755..2423
                     /locus_tag="VF531_10280"
                     /inference="COORDINATES: protein motif:HMM:NF044297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714401.1"
                     /translation="MRGGEGLTQAIEYNFFIDGDDVYVPFYNDCTATLRFNDETAVLE
                     ERSDYPYYGNTSFEVLKSSVKMAKKLKFFAPSWTQTEKITVYLNGKACKTSFDHQFIT
                     VESQLKTGDIIGFDLGLSVRTEDAVFKNSVKGYHKFCYGPMLLGYKTATAINSLEGNS
                     FENYAESKYKPAGKLKLAKNVKLVRQSKSEFAVADSSVILSCLCDIKDLTKEDSMRQL
                     LFKE"
     gene            complement(2535..2759)
                     /locus_tag="VF531_10285"
     CDS             complement(2535..2759)
                     /locus_tag="VF531_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714402.1"
                     /translation="MDQSELDQIFLCPRCGVDTLHYIAKRNQDLVGIVCAHCHTPSLV
                     KKEVLTYHQLKWEDELRQILNNLDNPFDDP"
     gene            2974..4059
                     /gene="dprA"
                     /locus_tag="VF531_10290"
     CDS             2974..4059
                     /gene="dprA"
                     /locus_tag="VF531_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7714403.1"
                     /translation="MEVEEIQYWLRWNRVSGIGPFRFYKLLEHFGSMKDAWNAPLEEV
                     NPIIGSKAEAALAESRNWDPQVELDKIARLGFGIYCQADPGYPSLLKQISDPPPVLYV
                     NGSFQYGDDIALAVVGTRTPSPLGAHHARELAYNLGKQGLTIVSGMARGIDAEGHRGA
                     MAAGARTIAVLGCGLNIAYPPEHRELMDAIAQNGAVISEFPLGTEPLATNFPARNRII
                     SGLSLGVVVVEAAQDSGSLITAGFALEQGREVFAMPGNIGNEGSRGPHKLIRQGAKLI
                     EDYRDILSELAIPHLSAPELAAAGVEQFSNIEQQVLAVMSREPLHIDQILRRLDLVSS
                     QLSAILLQLEMKGAVKRFPGQLYLRIK"
BASE COUNT         1156 a          901 c         1068 g         1060 t
ORIGIN      
        1 tgacgattcc ttcctaagcc tgcttaaagc ggccggaatg gagaacaact ggaagatggc
       61 cccggtaggc ggggagatgc ggccggagat ccaggtcagc atgtggaaat atactccgcc
      121 aaagggactg gaagattaca acgcttgtgt tgatttgact catgcgtcgt ggttactaga
      181 ccaaggagta ttcgatcagc cgccgaccgg tttaaaatgg tgccgggcaa tggaagcagc
      241 ccgccgtctg ggttacgaat tgttcgttcc gaaatcgttc ctcttttcta ccattaaaaa
      301 agatacaacg attaagctgg gtgtccaaat tcaaaacacc ggcatagccc cattctatta
      361 tgattggacg gttcaactcg gaatccgcaa ttcaagcggg gaagtcatca aaacctatga
      421 taccaattgg cggattagta aaatcatgcc cgggccgaaa ccaatggatt ttgagtggac
      481 gatcgatcgg cccggcctgg aagtcggttc gtacaccatg gtgatgcggg tggtcaaccc
      541 cttgaaaaac ggtaaaaagt tgatgtttgc cgatgttgag caaggtgaaa acggttggtt
      601 ggatctgggc ggtttccagg ttaccaaggg atgacaggca ggtcattaac ggtataaatt
      661 gatttgcagt cgtttggcta ttgaaaatta gggggaacca taagatgaac agcataagct
      721 tggccgatat cagcatcaag ggagaattag ctgtcagggc aggactaaac tatgccagac
      781 ttgaaggaca atggtacagg cccgatgagg tctttcaggc cgacaaacac ggttggcccg
      841 ccgattggga agggcgcatt atccttgccc tgaccttgct cgctcaaagt acccaccgta
      901 cccctgccta ccttgacagg ataattgcct tgctaccgga acacttaaat cgtaagggct
      961 atttcgggcc gatagtaccg gaagggaaat tcgatgaaca gcatttatcc ggccatagtt
     1021 ggatcttaag aggattaatc gagcactatt attggaaaaa ggatgagcac gtaaaacaaa
     1081 ttctggaaac catgctgcac aattatattc ttacggcgaa aggatattat gcccaatatc
     1141 ccattgaccc ggcgacccgg tttcaggacc cggtatggat actttcgaaa ttgcagacca
     1201 aaaccaagca tcatgcggaa acttccgatg cggggtgcgc ttttatactt atcgacgggg
     1261 ttactcaagc ttaccagtta tttcgctggc ctgaattaaa agagttcgcc gatgaaatga
     1321 ttcagcgttt catggagatt gatttaatga agttgcacat ccagacccac gcgactctca
     1381 gtagcttaag aggtatcttg cggctgtatg aattggagaa cgacgccgct taccttcaaa
     1441 aagcccaaaa agtgttcgat tattataaag atgaggcctg gaccgaagct tatggtaatt
     1501 ataattggtt tggtgcaccg aggtggaccg agccatgtgc gatcattgat tcatttattg
     1561 ttgcaacgag tctttggaaa tataccggca atcctcaata tctggaggat gcccaccgca
     1621 tatactataa cgctttatgt cacggatacc ggggtaatgg ttcatttggc acggatcgct
     1681 gctgaggagc ccaagaaacc gaggacaatt catttattac tcccattaat tatgaaacat
     1741 actggtgttg cacgatgagg ggcggcgaag gcctgacgca ggcgatagaa tataatttct
     1801 ttatcgatgg agatgatgtc tacgtacctt tttataatga ctgtaccgct actttacgct
     1861 ttaatgatga aacggccgta ttggaagaaa ggtcggatta cccttattac gggaatacca
     1921 gttttgaagt gttgaaatct tcagtcaaga tggctaagaa gttgaagttt tttgcccctt
     1981 catggaccca aacagaaaag attactgtgt atctcaatgg taaagcatgc aaaaccagct
     2041 tcgaccatca gtttattaca gttgaaagcc aattgaaaac cggggatatc atcggatttg
     2101 accttgggtt atcagtgaga actgaagatg cggtatttaa aaatagtgtc aaaggatatc
     2161 acaaattttg ctatggtccg atgttgttag ggtataaaac cgcaaccgca attaactccc
     2221 tggaaggcaa ctcttttgaa aattatgccg agagtaaata taaacctgca gggaagctca
     2281 aactcgccaa aaatgttaaa ttggtacgcc aaagtaagag cgagtttgcg gtggcggatt
     2341 catccgttat tctttcttgt ctatgtgaca tcaaggattt gaccaaggaa gacagtatgc
     2401 ggcaattgct gtttaaagaa tgaattaggg tcatttgagt tcattaagca ttgcatgtac
     2461 aggatttacc gaaagggcta tactgccaag cagttaagcc cttttctcgg tttagacaga
     2521 tgggaaagga cttctcaagg atcatcaaaa ggattgtcca gattattgag gatctggcgt
     2581 agttcatctt cccattttag ttgatggtaa gtgagtacct cttttttaac gagagagggg
     2641 gtatggcagt gggcacagac aattcccacc agatcctgat tgcgtttggc gatatagtga
     2701 agcgtatcca caccgcaccg ggggcaaaga aagatctgat ccagttccga ttggtccaca
     2761 gcagttcact tccttgagat aaaagtgcgc acgcgagccc tcttctctcg caatctccac
     2821 ctctattgtg tacctaaaaa atcgaatcat gctggcaaca atagtcaggt accagccgga
     2881 acattcggaa aatttaaact agtcgttcac aaaggcagga acggtttgaa ccctccacga
     2941 attatcaaat agattgaacg gggaggtgtt caaatggaag tagaggaaat tcaatactgg
     3001 ttacgttgga accgggtctc cggaatcggg ccgttccgtt tctacaagct gcttgaacat
     3061 ttcgggagta tgaaggatgc ctggaatgca ccgcttgaag aggtgaatcc gattattggc
     3121 tcaaaagcag aagcggcttt ggcagaatcg cggaattggg atccccaggt ggagctggat
     3181 aaaatcgccc gtttggggtt tgggatatat tgccaggcag atcccggtta tccatctcta
     3241 ttgaagcaga ttagtgatcc cccgccggta ctttatgtga acggtagttt tcagtatggc
     3301 gatgacatcg cacttgcggt ggtgggtacc agaacccctt cacctctcgg agcccatcac
     3361 gccagagaac tcgcctataa tcttggaaag caggggttaa caattgtgag cggcatggcc
     3421 cggggcatcg acgccgaggg gcaccgcgga gcgatggctg ccggagctcg gaccatcgcc
     3481 gtcttgggtt gcggcctcaa catcgcctac ccgcccgaac atagggaatt gatggatgcc
     3541 attgcccaaa acggagcagt aatctcggaa tttccccttg gcaccgaacc gctggctacg
     3601 aatttcccag cccgaaaccg tatcatcagc gggttaagct tgggcgtagt cgtggttgaa
     3661 gccgctcagg atagcggttc attgatcacg gcgggttttg ccttggaaca gggcagggag
     3721 gtctttgcaa tgccgggaaa tattggtaac gaggggagca ggggtccgca taagttgatt
     3781 cggcaaggag ccaaactgat tgaggattac cgggatatcc tgagtgaatt ggctattcct
     3841 catttatctg ccccggaact tgcggccgcc ggagtcgagc agtttagcaa cattgaacaa
     3901 caggtattgg cagtaatgag ccgggaacca ttgcatattg atcagattct acgccgcctg
     3961 gacctcgtat catcccaact tagcgcgata ttgcttcaac tggagatgaa aggagcggtt
     4021 aaacgttttc caggacagtt atatttaagg atcaaataac taactttaca aacgaaccag
     4081 aaatgatata atagggaagg ttacggggtt ttccgccatt ttacggcact tttccaaatt
     4141 ggaaaccttt tttcattaag atccgccttt cttttaccga aaata
//
LOCUS       DASVMB010000362        11661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_339092,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000362 DASVMB010000000
VERSION     DASVMB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339092"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF531_10295"
     CDS             complement(<1..206)
                     /locus_tag="VF531_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714404.1"
                     /translation="MRKFIYFLLLIGFTLFSILPFYVVAMMATHSTAEIFSGLVLSPG
                     THFMDNMKMIFVNKFQYCYLNSIIV"
     gene            complement(209..1156)
                     /locus_tag="VF531_10300"
     CDS             complement(209..1156)
                     /locus_tag="VF531_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714405.1"
                     /translation="MNQSRNKPSFSERLKRHCWPYLFILPFLLIYCAFNLFPVIYTFL
                     ISLTNWDALFLNDRKFIGLANYVQLFTKDPYFFKSLVNTIGFMIGYIPFIIMLGLFLA
                     VILFELPVLKRTFQTLNLLPYITTPVAIGFIFSYLFDWSNGLVNRILITLHIIPDGIN
                     WLGAGGLARGIVILMIFWKNLGYFFMIYLAGLTGIPAELNEAATVDGASKRQIFFHIT
                     VPLLKPITAFLVITAIIGGFQLFDEPFLLFSGGLTGNGLRIVGGPDRACLTTVWYFYD
                     TAFRSTSRLGMGAAISYGLFLFVFIFSILGVKIFNRKEE"
     gene            complement(1318..2604)
                     /locus_tag="VF531_10305"
     CDS             complement(1318..2604)
                     /locus_tag="VF531_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714406.1"
                     /translation="MLRRNWKVLLSLLVLVVLLGSLTVQGATGISKDYSCTLTTWGWD
                     ENYWNHVVAGFQKNYPNIKFEYTPVANGDYLQKLQTSLAAGTPLPDIPWAIIDSRGRA
                     FELDMWEPLNKAPYKMNPKDVFDYLIPIMKNSKGDICGIEQGINPAAMAYKRDLAKKY
                     FGTDDPEKLAKLLPDWNTFVAKGKEVAAKSGGKVYMMQGIGDIYQIARDQNPTPWVVK
                     DTVNATKIFKPLLQRVVEFRDAKILDKLSAWTPAWYASFGQDKYIFAGCAIWSPQYVI
                     EPNDPNGKGNWGLMRAPGGNFSWGGTTLGISKQCKHKAAAWEFVKYATLSVDGAKAAH
                     EVGFLTSCKAAYKDPEFASIRNEWFGGQDIGKYWINEVAPNIKIRPMTKYDNIIKDSL
                     DLIVTSLNNDTKMTVPDALPKLIQEIKNRAPELTVK"
     gene            complement(2709..4481)
                     /locus_tag="VF531_10310"
     CDS             complement(2709..4481)
                     /locus_tag="VF531_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7714407.1"
                     /translation="MKLGIKAKILGATGLVVMISLMLSGIWAYYYFQKIFKEKAIKDD
                     AGKIDQIAQQMEYQIDDIRKYATSMIIIPEVQDFIKQSRYASYYEQLVSARRALGLMM
                     NQLFLREYIHSAAVITISGSVWYTDLHTMPHDYFKSKLKESWYQRYLGKKDQFYFSDP
                     YQISNYSRGTAAEPVISYIVQMHDVDQSEKINGRLFLNVFLSHFEKNILTNSKEYDAF
                     YWVNQDGTIIYQRNSTPKDIRIEEVRSQIKASDEKTSVIENAKGYLILNHPLSNGWRL
                     ISFTSNRKMLQRINFIFIFFLCFTLISLAVIIVVVFPIILRITNPITELTEAMKQVAS
                     GNLDTSLQITSGDELETMANGFNRMVVDLKNHLAKSVEYERVKRKMEYDILLSQINPH
                     FIYNVLDTVIYMARKKKDQDTVNLLGSFIRILQDGIKVGGDGLLTTVKQEVEMVNHYV
                     TIQEYRYRGRFTMKWEVEETVVNLSIPRSLIQPLVENALYHGICPKDETGVIKVVIRR
                     DREDLEIRVEDDGVGMEKGLIAKLLAGEPIYEPESRMRPIGIANIRDRIRYLYGKDYG
                     LTIESTVGKGTVITVKLPMNSMGS"
     gene            complement(4465..6060)
                     /locus_tag="VF531_10315"
     CDS             complement(4465..6060)
                     /locus_tag="VF531_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7714408.1"
                     /translation="MVKMMPNQIKVMIVDDEILAIEDLEQLIPWRQHGFEIVATATGS
                     QKALELYEKHRPQIIFVDIRMPAMDGLEFSRRVLAYRYPVRIVLLTAYKDFEYAQQAV
                     ALGVSNYLLKHDVNEASLLKELNKLRDDLENTFRRDAIVKQRLIKRILTGESPGDWSG
                     GELRLINQNPENLALLLIHPVTPYLERFKAISGMHPVKISDIKEVPLPDGIYCADGFD
                     LDELGFIVLVGMKRVNSQAEERERLYQYALALQNYLCEANHQNVAIIISRSCAQIEVL
                     PLLYQRMIRARQYLVFYHPKQILCSDDLPVITGLQTTDIDRDLQILAQSLRETDCSGV
                     QSAIGELFQGVLSSRNPEQLKRICDELLERLEHYRNHHGLSSLMELHKQGKLNLESLS
                     YYTGLQDWFRDQFDGTIRQVFELNQGRYSKKVQQAISHIRQHYAADLTVENVAGALGI
                     SGVYLSQLFKKETHRTFLEYLTEHRIKIAKDLLQSGDYKIYEVAAMVGYKTSQYFSHV
                     FRKVTGVYPVDYREGATRNETGD"
     gene            complement(6296..6961)
                     /gene="deoC"
                     /locus_tag="VF531_10320"
     CDS             complement(6296..6961)
                     /gene="deoC"
                     /locus_tag="VF531_10320"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX7714409.1"
                     /translation="MVTYEEIAAMIDHSLLKPFLTDKEIEEGCRLADQYQVASVCVRP
                     SDVKLAKKILQNSKVRVTTVIGFPHGTTTTLTKITEAREAMENGAVELDVVINIGKLK
                     SKNHEYVKQDLQAVTDVAHDKQVLIKVIFENCYLSEEEVIAACKICNEVGVDFVKTST
                     GFGSGGAEDRDLQIMRENADPQIQIKAAGGVRTLERAIEVKALGCTRFGCTATVGILE
                     RLK"
     gene            complement(7060..9393)
                     /locus_tag="VF531_10325"
     CDS             complement(7060..9393)
                     /locus_tag="VF531_10325"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7714410.1"
                     /translation="MSNYLIDARNQKQPIDKGHLRISGTNPTGELLSCNSYFLEKNGR
                     PYFPIGGEFHFSRYPYRQWEEEIIKIKLAGIDLIPTYIFWIHHEEDEGRFDWTGNKNL
                     RDFVDLCAKQQMRMILRIGPFAHGECRNGGLPDWLYGRPFEVRSNDPGYLHYVGRFYR
                     EIGRQVKGLLYKDGGPIIGVQLENEFTASSAKWELTTLQGDEWIPSGSGGAEHMRILK
                     QLALEAGLDVPLYTCTGWGNAPIVEGEMLPMWGGYPFQPWLLYWNSQLTSHPPTVNYI
                     FRDYHRNDPRYLDASYPTEQYPFACCEIGGGMQPWYNYRFVVPPESVEAMVINRLAGG
                     CNFLGYYVFHGGSNPSGKHSYLNERCVPKICYDFQAPLGEFGQVRDSYRYLKPLHLFC
                     RDFAELLCPMSTVLPDVAGGMQPADTETLRFGARVAVNSGFLFLTNYQDHLTTKDQEN
                     VRITLILPGEELTVPAKNSFTLRSGVNAILPFNLNLNGVLLKWATTQLVTSLRVDDEP
                     YYFFFVPEGMAGEYCFDNSTFAAITVEEGLIGDEPNRTYISVSPGMKCKITITDRKGQ
                     RVHICTLTREQILHFWKIELWGRERVIISPDDILAGAGYLELRNRERACFELDIFPAT
                     DGNLLVDGKEIPNRGEGSFRHYRFDLPQKATLAQWEKVAPGKAVIRIPREAFTTVNEL
                     FLRIDYLGDVGNLFIDGKLIADDFCNGETWEIGLKRFYPEIANKELCLTVSPLRKGHT
                     TLNNATLAVKEDFIGEQIAEIRSVQLIPEYRAVVLKD"
     gene            complement(9505..10005)
                     /locus_tag="VF531_10330"
     CDS             complement(9505..10005)
                     /locus_tag="VF531_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7714411.1"
                     /translation="MDPEGLVTAIIEIPQGSVNKYEFDQKTQRFRLDRVLYSPIHYPA
                     DYGFIPETLAADGDPLDILVFISHPTFPGCEIPARIIGGLVMADEKGDDQKILSVAAV
                     DPRFGDVDCLAKVPAHTLKEIEYFFSIYKDLEGKSCQVTGWLDRADAIELWQQYRIRS
                     EEPILT"
     gene            complement(10100..11260)
                     /locus_tag="VF531_10335"
     CDS             complement(10100..11260)
                     /locus_tag="VF531_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEX7714412.1"
                     /translation="MIESTPERDYWVDTLVGISGPVLKALAGKRLMCSMPTGGAAGER
                     EERREFLCLEALGRTLVGIAPWLEGDEGSAAENERREEYRILARQAIAAATDPASPDC
                     ANFRSDFQPIVDAAFLAHALLRAPRELWHKLDNPVKVNLIRGLKATRTRKPFFCNWLL
                     FAAMIETFLAKAGEDWDPMRVDYAIKQHEQWYLGDGVYGDGPEFHWDYYNSFVIQPML
                     VDIVSQIGSEYAEWNELKQVILQRASRYAAVLERLISPEGTFPPIGRSLAYRFGAFQL
                     LAQMALEKNLPPELKPSQVRTALTAVIKRVMRIPGTFDDQGWLKIGFCGSQDGIGESY
                     ISTGSLYLCTTAFLPLGLSPDEPFWKDPARDWTAKRIWSGENLPADHAINKE"
     gene            complement(11326..>11661)
                     /locus_tag="VF531_10340"
     CDS             complement(11326..>11661)
                     /locus_tag="VF531_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HEX7714413.1"
                     /translation="DYICYWDLVFTSGDEERDSSAAAIAACGLLELAKHLPLTDVYRT
                     IYENAAMHMLYNLALKYTTAGMKSNGVLGHAVYSKPNHEGVDECVIWGDYFYLEALTR
                     LLKDWRLYW"
BASE COUNT         2960 a         3085 c         2615 g         3001 t
ORIGIN      
        1 acgatgatac tgttcagata acaatattga aatttgttga cgaagatcat cttcatgtta
       61 tccataaaat gagttcctgg cgaaagcacc agtcccgaaa agatctcggc tgtggaatgg
      121 gtggccatca tggcaaccac ataaaacggc aagatcgaaa acagcgtgaa tccaatcagc
      181 agtaagaagt agatgaactt tctcatggct attcctcttt ccggttgaaa atcttaactc
      241 ccaggatgga aaagatgaaa acgaacaaaa atagtccgta cgaaatcgcg gctcccatac
      301 ccaaccggga agtactgcga aacgcagtat cataaaaata ccaaaccgtc gtcaggcaag
      361 cccggtccgg accgcccacg atccgcaacc cgttgccggt caaccctccg ctaaacagga
      421 ggaacggttc gtcaaacagt tgaaatccgc cgatgatagc ggtgatcact aaaaaggcag
      481 taatcggttt gagcaggggt acggtgatat gaaaaaagat ctgacgtttt gaagccccat
      541 caacggttgc cgcctcattc aactcagccg gtatcccggt taagcccgcc aaatatatca
      601 taaaaaagta cccgaggttt ttccagaata tcatcaaaat cacgatccca cgcgccaatc
      661 cgccggcgcc caaccagttg atgccgtcgg gaatgatatg gagcgtgatg agaatgcggt
      721 tcaccagtcc attagaccag tcaaacaggt aggaaaagat aaatccgatt gctaccgggg
      781 tcgtgatata gggcaacaag ttcaaagtct gaaaggtcct cttcagaacc ggcagttcaa
      841 agaggatcac ggctaaaaac aatcccaaca taatgatgaa cggaatatac ccgatcatga
      901 aaccgatggt gttcaccagt gatttgaaga aatagggatc cttcgtaaac aactggacat
      961 agtttgccag tccgataaat ttgcggtcat tcagaaacaa agcatcccaa ttggttaggc
     1021 tgatcaaaaa cgtatagatc acgggaaaca aattaaaggc acagtagata agcaagaacg
     1081 gcaggatgaa aagatacggc cagcagtgtc tttttaacct ttccgagaaa gacggtttat
     1141 tccgactctg attcatcttt gttgttcccg cctgaagttg attggtaact gaatcggcac
     1201 tcatgtcgat accaactttc ctttggtttc atcatggaca tcaaaaaggg taaagttatg
     1261 gcaactatat gtgaaacgaa ctatagttgc cataactttt ctcgcgttta gcttaaatta
     1321 ttttacggtc aattccggag ctctgttctt aatctcctga atcagcttgg gaagagcatc
     1381 gggcacagtc atttttgtgt cattattcag gctcgtgacg atcagatcca atgaatcctt
     1441 aattatatta tcatacttgg tcatcggccg aatcttgata ttcggggcga cctcgtttat
     1501 ccaatacttg ccaatatcct gaccgccgaa ccattcgttc cgaatcgaag caaactccgg
     1561 atctttatat gcagccttgc aggaggtgag gaaaccgacc tcatgggcag ccttggcgcc
     1621 gtccacactc aatgtggcgt acttgacaaa ctcccaagca gccgccttat gtttgcattg
     1681 tttggagatg ccgagggtgg ttccacccca gctgaaatta ccgcccgggg ctctcatcaa
     1741 accccagttg cctttgccgt tgggatcgtt gggttctata acatattgcg ggctccaaat
     1801 ggcgcaaccg gcgaaaatgt atttgtcctg accgaacgaa gcgtaccaag cgggagtcca
     1861 agccgacaat ttatccagga tcttggcatc cctaaactct accaccctct gaagcagcgg
     1921 tttaaagatc ttggtggcat tgacggtatc tttgaccacc caaggagtgg gattttgatc
     1981 ccgggctatc tgataaatat ctccgatgcc ttgcatcata taaactttcc caccgctttt
     2041 ggccgctact tccttgccct tggccacaaa ggtattccaa tcgggtaaca gcttggccaa
     2101 tttctccgga tcatccgtgc cgaaatactt cttggccaaa tcacgcttgt acgccatggc
     2161 cgccggattg atcccctgtt caataccgca gatatctccc ttggagttct tcatgatcgg
     2221 aatcagataa tcaaagacat ccttcgggtt catcttatag ggggccttat tcaacggttc
     2281 ccacatatcg agctcgaatg ccctgccgcg ggagtcgatg attgcccagg gaatgtccgg
     2341 taacggtgtt cccgccgcca gactggtttg aagcttttgt aagtagtccc cgttggctac
     2401 cggagtatac tcgaatttga tattcggata gttcttttga aatccggcca caacgtgatt
     2461 ccaatagttt tcatcccaac cccaggtggt tagggtgcaa ctatagtcct tggagattcc
     2521 cgtcgcgcct tggacggtta agctccccag gaggactacc agaaccaaaa gagataaaag
     2581 taccttccaa ttccttcgta acatgacttt tcctcctttt ttggtttgtg ccttcctttt
     2641 tgtaattgaa ttgtaactcc cgggtaacaa aagtgaacca tcacaaagcg ataattttgt
     2701 ataaaatcct atgatcccat cgaattcatc ggcagcttga cggtgatgac cgtccccttg
     2761 cctaccgtgc tctcaatcgt taaaccgtaa tcttttccat agagataccg gatcctgtcc
     2821 ctgatattcg cgattccaat cggccgcatc ctggattccg gttcgtagat gggctcgccc
     2881 gccagtaact tggcaatcag ccctttttcc atccccactc cgtcatcttc cacccggatt
     2941 tcaagatctt cccgatcacg gcggattacg accttaatca ctcccgtttc atccttgggg
     3001 cagataccgt gatacaaggc attctctacc aacggctgaa tcaatgacct gggtatggac
     3061 aagtttacta ctgtctcctc aacctcccac ttcatcgtga aacggccccg gtaacggtat
     3121 tcctgtatcg tcacatagtg gttcaccatt tccacctctt gcttgacagt agtcaataat
     3181 ccgtccccgc cgaccttgat cccatcctgt aaaatccgga taaaggatcc caacaaattg
     3241 acagtgtcct ggtccttctt tttccgggcc atgtagatca cggtatccag aacattatag
     3301 ataaaatgcg gattgatctg cgaaagcagg atgtcgtatt ccatttttcg tttgactctt
     3361 tcatattcca ccgacttcgc cagatgattc tttaaatcca ccaccatacg gttgaacccg
     3421 ttggccattg tttcaagttc atcaccgctg gtgatctgga gtgaagtgtc aaggtttccc
     3481 gatgcaacct gtttcatcgc ctcggtaagt tcggtgatgg gattggtgat ccttaggata
     3541 atcgggaaaa cgaccacgat gatgactgcc aggctgatga gggtaaaaca aaggaagaaa
     3601 atgaagatga aattgattcg ctgcaacatt ttgcggttgg aagtaaacga gatcagccgc
     3661 cagccgttgc tcaacggatg attgaggatc aaataccctt tggcgttctc aattacgctg
     3721 gttttttcat cgctcgcttt gatctgcgat cggacctctt cgatccggat atccttcggt
     3781 gtagaattcc gttgataaat gatggtccca tcttgattga cccaataaaa ggcgtcatat
     3841 tccttgctgt tggtcaggat gttcttttcg aagtggctca aaaaaacatt caaaaagagc
     3901 cggccgttga ttttttcgga ttgatccaca tcgtgcattt ggacaatgta actgatcacc
     3961 ggttcggcgg cagtaccccg ggagtaattg gagatttggt aggggtcgga gaagtaaaat
     4021 tggtctttct tgcctaaata tctctgatac cagctttcct tcaatttact cttaaaatag
     4081 tcatgcggca tggtatgtaa atcggtgtac caaaccgaac cgctgattgt aatgacggca
     4141 gcgctatgga tatattcccg gagaaaaagc tgattcatca taagccctaa cgccctccgg
     4201 gcgctgacca gctgttcgta gtaacttgcg taacggctct gtttgataaa atcctgaacc
     4261 tccgggataa tgatcatact ggtcgcatat ttccggatat cgtcgatctg atactccatc
     4321 tgctgggcga tttgatcgat cttccccgca tcatccttga tcgccttctc cttaaaaatc
     4381 ttttgaaaat aatagtacgc ccagataccg ctaagcatca gggatatcat aactaccagg
     4441 ccggttgcgc caagaatctt ggccttaatc cccagtttca ttgcgagtgg ccccttccct
     4501 gtagtcaacc ggatataccc cggtaacctt ccggaaaaca tggctgaaat attggctggt
     4561 cttgtacccc accatggcgg caacctcgta tattttataa tcgccacttt gcagcaggtc
     4621 cttggcaatt ttgatccggt gctcggttaa atactccaaa aaggtccgat gggtttcctt
     4681 tttaaacaac tgactgaggt agaccccgct gattcccagg gccccagcca cgttttcaac
     4741 ggtcaaatcc gcggcgtaat gctgccggat atgggaaatg gcctgctgta ctttcttgga
     4801 ataccggcct tgattcagtt caaaaacttg gcggatcgtt ccgtcaaatt gatcccggaa
     4861 ccaatcctgc aaaccggtat aatagcttaa agactcgagg tttagctttc cttgtttatg
     4921 aagctccatc agagaagata atccgtggtg attgcgatag tgttccaatc gttcgagaag
     4981 ctcatcacag attcgtttta actgttcagg gttccgggag gaaagaactc cttgaaaaag
     5041 ttctccaatg gctgactgca ctcctgaaca atccgtctcc cgcaggcttt gggccagtat
     5101 ttgaagatcc cggtcaatat cggttgtttg aagcccggtg ataaccggta agtcatcgct
     5161 acaaagtatt tgtttgggat ggtaaaaaac cagatattgc cgggcccgga tcattctttg
     5221 atagagtagg ggcaaaacct caatctgcgc gcaggagcgg ctgataatta tcgcaacgtt
     5281 ttggtgattc gcttcgcata aataattctg caaggccaag gcatattgat aaagtctctc
     5341 ccgctcctcc gcctgactgt taacccgctt catcccgacg aggacaatga atcccaattc
     5401 atcaaggtcg aacccatccg cgcaatatat gccgtccgga agcgggactt ccttgatatc
     5461 cgaaattttt accggatgca tcccgcttat ggccttaaat cgctcaagat acggggtgac
     5521 aggatgaatc aacagtaacg ccaggttctc cggattttga ttgatcaaac ggagttctcc
     5581 gccggaccag tcgcccggtg actccccggt caggatgcgt ttgattaacc gttgtttgac
     5641 aatggcatct ctccgaaacg tgttttccag gtcatccctt aatttattga gttctttgag
     5701 taagctcgct tcattgacat catgcttcaa gaggtaattg gaaaccccga gggctacagc
     5761 ttgctgggca tactcaaaat ccttgtatgc ggtcaataaa acgatcctca ccgggtaccg
     5821 atatgccaaa actctgcggc tgaactccaa cccgtccatg gccggcatcc gaatatctac
     5881 gaaaataatc tgcggccggt gtttttcata gagttccaag gctttttggc taccggtggc
     5941 ggtggccaca atctcaaagc catgctgccg ccagggaatg agctgctcca gatcttcgat
     6001 ggccaggatt tcatcatcga cgatcatcac tttaatttga ttgggcatca ttttcaccat
     6061 gatttccaat ttctttaaat aaatcctacc agtattttaa ttgtaacgag ttggtaaaat
     6121 aatgtaaagt gagccgattg ggaagtgatg ataatacttc ggcattattt ataccagtat
     6181 accttttaga ttcacttttc gatgatatat aaaaaacgtt accaatcaga tgattgataa
     6241 cgtttcccgt ttcatttcgc aatcctgcga ccttgcaacc atttccgctt cattatcatt
     6301 ttagtctttc caaaatccca accgtagccg tacaaccgaa ccgcgtacaa ccgagcgctt
     6361 ttacctcgat cgccctttcc agcgttctaa ccccgcccgc cgctttaatc tgaatttgag
     6421 gatccgcatt ctcccgcatg atttgtaaat cccggtcttc cgctccgcca cttccgaagc
     6481 cggttgaggt tttaacaaaa tcaacaccca cctcattgca tatcttgcag gcagcgatga
     6541 cctcctcctc gctcagataa cagttttcaa agattacctt gatgagaacc tgtttgtcat
     6601 gagcgacatc ggttaccgcc tgaagatcct gtttgacgta ttcatgattt tttgacttca
     6661 atttaccgat attgatcacg acatccaatt caacagcacc gttctccatc gcttcccggg
     6721 cctcggttat cttggtcaag gttgtggtgg tgccatgagg aaaaccgatg actgtagtca
     6781 cccggacctt actgttttga aggatcttct tggcgagttt gacatcggac ggccggacgc
     6841 aaaccgaggc gacttgatat tgatcagcga gtcgacatcc ttcctcgatc tccttgtcgg
     6901 tcagaaacgg ttttaacaaa gaatggtcaa tcattgcggc gatttcttcg taagtaacca
     6961 tttttacctt ctcacttctt tgcattaggt gatgtgcgca agacactttg cctttcctac
     7021 tattttacca tgaatattat gagtgatcta gcttgaacat taatccttca acacaaccgc
     7081 cctatactcg ggaatcagtt gaaccgaacg gatttccgcg atttgttccc ctataaaatc
     7141 ctctttgaca gcgagggtgg cgttgttgag cgtcgtatgt cccttacgca agggtgaaac
     7201 agtaagacac aactccttat tcgcgatctc cgggtaaaac cgctttaggc caatctccca
     7261 ggtctccccg ttgcaaaaat catccgcaat cagcttcccg tcgataaaca gattaccgac
     7321 atcccccaga taatcaatgc ggagaaacag ttcattcact gtggtgaacg cctctcgcgg
     7381 gatccggatc accgccttgc ccggagctac tttttcccat tgggccagcg tcgctttttg
     7441 cggaagatca aaccggtaat ggcgaaaact cccctccccg cgattgggta tttccttgcc
     7501 atccaccaaa agattgccat ccgtggccgg aaagatatcc aactcaaaac aagcccgttc
     7561 cctgttgcgg agctccaggt acccggcacc cgccagaata tcgtccgggg aaatgatcac
     7621 tctctccctg ccccagagtt cgattttcca aaaatgaagg atctgctccc gagttaaggt
     7681 acagatatgg accctctgcc ccttccggtc ggtgatggtt atcttgcatt tcatgccggg
     7741 agatacggaa atataagtcc ggtttggttc atcaccgatc aaaccttcct ccactgtaat
     7801 cgcagcaaaa gtggagttgt cgaaacaata ctcaccggcc attccctccg ggacgaagaa
     7861 aaagtagtaa ggttcgtcat cgaccctgag cgaggtcacc agttgggtgg tagcccattt
     7921 gagcaagact ccgttgagat tcaggttaaa aggcagaatg gcattgacac cgcttcgcaa
     7981 cgtaaaactg tttttggcgg gcacagttag ttcttccccg ggcaaaatga gagtaatccg
     8041 gacgttttcc tgatccttgg tagtcaaatg gtcctgataa ttggttaaaa agagaaatcc
     8101 cgaattaacg gcgaccctgg cgccaaaacg tagggtttcg gtatcggccg gttgcatccc
     8161 gccggccaca tccggcaaaa cggtgctcat cgggcagagc aactccgcaa aatcacggca
     8221 gaaaagatgc aacggcttga gataccggta tgagtcccgg acttgaccga actccccaag
     8281 cggtgcttga aaatcgtagc agattttggg tacgcagcgt tcgttaagat aggagtgttt
     8341 gccgctggga ttggagccgc cgtgaaacac ataataaccc aaaaaattac accccccggc
     8401 cagccggttg atgaccatcg cctccacgct ttccggcggg accacgaacc ggtaattata
     8461 ccagggctgc atcccgccgc caatctcgca acaggcgaag gggtattgtt ccgtcgggta
     8521 ggacgcatcc aggtatcggg gatcgttccg gtgataatcg cggaaaatat agttgacggt
     8581 cggaggatgc gaggtcaact gcgaattcca gtaaagcagc cacggctgaa aggggtatcc
     8641 gccccacatc gggagcatct ccccctctac tatcggggcg ttgccccaac cggtacaagt
     8701 atagaggggg acatccagac cggcctccag ggccagttgt tttagaatcc gcatatgttc
     8761 ggcaccgccg gatccgctgg gaatccattc gtccccttgt aaggtggtca gttcccattt
     8821 ggcgctggat gcggtaaatt cattctcaag ctgaacaccg ataatcgggc cgccgtcctt
     8881 gtataacagg cccttaacct gtctgccgat ctcccggtag aagcgcccga cataatgaag
     8941 atatcccgga tcattggacc ggacctcaaa agggcggccg tacaaccagt ccggtaagcc
     9001 gccgttacgg cactcgccgt gagcgaaggg accgatccgc aatatcatcc gcatctgttg
     9061 tttggcacag agatccacga aatcgcggag gttcttattg cccgtccaat cgaaccggcc
     9121 ttcatcttcc tcgtggtgga tccagaagat ataggtcgga atcagatcga tcccggccag
     9181 cttgattttg ataatctctt cctcccattg gcggtacggg taacgtgaga aatgaaattc
     9241 accgccgatc gggaaataag gccgcccgtt tttctccagg aaataactgt tacagctcaa
     9301 caattctccg gtgggattgg ttccactgat ccgcaaatgc cccttgtcaa tgggctgttt
     9361 ttgattgcgc gcgtcgatta aatagttgct cataacgata cctctagacc attgcagtta
     9421 tttcaaatta tataaattat atcaaaaaag tactccatga tcatcaaagg ccgcccaagt
     9481 tccgggacgg ccctattgtt catattaagt tagaattggt tcttcacttc ttatccggta
     9541 ctgttgccaa agctcaattg catccgccct atccagccac cccgtgacct gacaggactt
     9601 gccctccaga tccttataaa tagagaagaa atactcgatc tcctttaggg tatgggctgg
     9661 aactttggcc aggcaatcga catcaccgaa acgggggtcg accgccgcta cgctcaagat
     9721 cttctggtca tccccctttt catcggccat taccaaaccg ccgatgatcc gggccggtat
     9781 ctcacaaccg ggaaaggtag ggtgggagat aaagaccaaa atatccaacg gatcaccgtc
     9841 tgccgccagg gtctccggga tgaacccgta atcggccggg taatggatgg gggagtagag
     9901 tacccggtcc agccggaacc gctgcgtttt ttgatcgaac tcatatttat tgacactgcc
     9961 ttgtggaatt tcaattatcg ccgtcaccaa gccctcagga tccattttcc tcaccccttt
    10021 atagcttggc tgctctccca tcattctaac caccaactta tttatatcgg ctaaaccttt
    10081 taccatttac tctttccctt tactctttat ttatcgcatg gtccgccggt agattctctc
    10141 ccgaccagat ccgtttggcc gtccagtccc tggcgggatc tttccagaac ggttcatcgg
    10201 gcgagagacc caagggtaaa aaagccgtgg tgcaaaggta aagactcccg gtagagatat
    10261 aggactcgcc gatcccgtcc tgacttccgc aaaacccgat tttaagccag ccctgatcat
    10321 caaaagtacc gggaatgcgc atgacccgtt taattacggc ggtcagtgcc gtacgaacct
    10381 gggacggctt tagttccgga ggaagatttt tttccagcgc catttgcgcc aatagctgaa
    10441 aggctccgaa tcgataagcc aacgagcgtc caatgggcgg gaacgttcct tccggagaga
    10501 ttaaccgttc caaaaccgcc gcgtacctcg aggctcgctg taaaatcacc tgcttaagtt
    10561 cattccattc cgcgtattcg gaaccgatct gcgaaacgat atcgaccagc atcggctgaa
    10621 tgacgaaact attgtaatag tcccagtgaa actcgggccc gtcaccataa acgccatcgc
    10681 ccagatacca ctgctcgtgc tgttttatgg cgtaatcaac ccgcatcgga tcccaatcct
    10741 caccggcttt ggccaggaat gtctcgatca tcgccgcaaa tagcagccag ttacaaaaga
    10801 acggtttccg ggtgcgggtg gcttttaaac ctcggatcag attgaccttg accgggttgt
    10861 caagcttatg ccataattcc cggggcgccc ttagcagagc gtgggcaaga aaggccgcat
    10921 caacaatggg ctgaaaatcc gacctgaaat tagcgcagtc cggtgatgcg ggatcggtcg
    10981 ccgctgcaat cgcctgtcgc gccaagatac gatactcctc gcgacgttca ttctccgcgg
    11041 cgcttccttc atcaccctca agccacggag cgatcccaac cagggttctt cccagagctt
    11101 caagacaaag aaactctctt cgttcctccc gttcaccggc tgctccgccc gtaggcattg
    11161 aacacatcaa gcgcttcccg gccaaggcct ttagcaccgg acctgatatc ccaaccagtg
    11221 tatcaaccca ataatctctc tccggcgtag actcgatcat tctcgttgac cctcttccac
    11281 aaagactcta cctgcatgaa aactcctctt tcacaaacct cctctttacc agtacaatct
    11341 ccagtctttc aacaaacggg tcaatgcttc cagataaaaa taatcccccc agatgacgca
    11401 ctcatcgacg ccctcatgat tgggcttact ataaaccgca tggcccaaga ctccgttaga
    11461 cttcattccg gcagtggtgt acttcaaggc cagattataa agcatatgca ttgcggcatt
    11521 ctcgtagatc gtccggtaaa catcggtcaa cgggaggtgt ttggcgagtt ccagcaatcc
    11581 gcaggcggca atggcagccg cggagctgtc ccgttcttcg tcaccggaag taaaaaccag
    11641 atcccaataa cagatataat c
//
LOCUS       DASVMB010000363        10793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_339711,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000363 DASVMB010000000
VERSION     DASVMB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339711"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF531_10345"
     CDS             <1..283
                     /locus_tag="VF531_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714414.1"
                     /translation="ISETHRLILHKLGIIAGQKFLAQLFKEIEAKFVEVVPVDWPDLA
                     QARVLINQYHDQDLSLTATCSAVLMHRNNISQIASYDRHFTVLQFEILP"
     gene            complement(302..898)
                     /locus_tag="VF531_10350"
     CDS             complement(302..898)
                     /locus_tag="VF531_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7714415.1"
                     /translation="MNEYDQLVAFIKELDRLKDTTRTAWTKEGRQESVAEHSWRLAMF
                     AMVLVDYFPGVDRDRVVRMSLVHDLGEAYEGDISAKIGVSKEDKLKREEAALRKLFAP
                     LPESIRSGFLALWREYNDGKSQEAKIVKALDKIETIIQHNQGDNPSDFDYEFNLQYGK
                     EYMFSEPVIQSIREIIDGETRRKVKDNKPALEGRRKDK"
     gene            complement(1089..2255)
                     /locus_tag="VF531_10355"
     CDS             complement(1089..2255)
                     /locus_tag="VF531_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7714416.1"
                     /translation="MTNLLIRLFVKDHQNIANFKVRERYGKFAGVVGIASNFLLFLIK
                     ITVGTLFNSIAIVADAVNNLSDSGSSLVTLLGFKISGKPADAQHPYGHARMEYISGLI
                     VSFVILVLGLQLIQSSVSKIMHPQVAEFSLITIVTLVIAILIKLWQCMFYRKIGKIIK
                     STTLIATSVDSQNDILATSAVLVATLVTRFTGYNLDGYMGVVVAVFIMITGVRLISDT
                     ISPLLGTAPTKELVDGIYQKILSYDGIIGLHDLTVHNYGAAQCFASVHCEVPAEQDIM
                     ISHDIVDNIERDFLKDRGIHMVIHLDPIVTNDVKTNQLKETVENLVKQISPEISIHDF
                     RVVWGVSHSNLIFDVVIPFDFQWSDDELVKLLTAKIYGINRNYHSVITIDHNYI"
     gene            complement(2420..2680)
                     /locus_tag="VF531_10360"
                     /pseudo
     CDS             complement(2420..2680)
                     /locus_tag="VF531_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
     gene            complement(2689..3081)
                     /locus_tag="VF531_10365"
     CDS             complement(2689..3081)
                     /locus_tag="VF531_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714417.1"
                     /translation="MNWFRRFMMGRYGGDQLSMVLIIFSAILTLIAQLAQLRLLAFIG
                     YVPLGIAVFRMLSRNTSKRSMENYKFAIRMSPVYSWFKKEQNRFRDRKTHKYYTCPKC
                     RQSLRVPKGKGKIIITCPKCKTEFPQRT"
     gene            complement(3156..4931)
                     /locus_tag="VF531_10370"
     CDS             complement(3156..4931)
                     /locus_tag="VF531_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714418.1"
                     /translation="MMNNRLVKESIANVIKKNISIIGLLVFVVGGAVILSLIPPQILK
                     NIIDNSLVPRKRDGLRTLAVAYMAVLLLIGIFDFMKEAVLTVLGQKITKEIRLKMMEK
                     LEKINAVFFSANESGTVVSRFTNDVDAVSSMFTGGIVGMIIDCFKIIGIVVSIWVFSG
                     KLGMVSLISLPVIYGITRLFQRRMLTAQIDNRSLVGKVNNHLAESLKNIRMIKSYSKE
                     DYMEENYAGLLLRNFQTVEKVNFYDSIFSPIIQLTRAAVIAVIVILSSKQPSYLGISL
                     GMVAASIELISNLFAPIETLGMELQNIQQAFSGIHRVNEFYHEPEDDPKNSQLKAEAI
                     VPRREEVSVSFHDLTFHYDEGTDVLQSINLRIHPGEKATFVGRTGVGKSTLFKLIMGI
                     LKPAKGSITINGIDVYTIPNSEKRKLFGFVDQSFHMIKGTVAEQISLQDKSITRGQIQ
                     NALDFVGLADYVASLENGLDTQVNGDSLFSQGQKQLLSIARAIVTNPPILLLDEITAG
                     LDSITEEKIVSVLQKAGDTYTILSISHRLSTMIASDTVVILENGKVKNSGSPEMLLQN
                     DDWYRSHIRLEKLTWDEVDLGMKLI"
     gene            complement(4921..6687)
                     /locus_tag="VF531_10375"
     CDS             complement(4921..6687)
                     /locus_tag="VF531_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714419.1"
                     /translation="MAKLKIEPMKQADRIINYWKKEKFVVACIVMFGLTFNGSIILGP
                     IYQGKLIDAIVRGDSLSAVGKLAAIYVALIGMIQFLRYFKRFYIRRFANNTSATMRLM
                     LYNNIMHKSTEELDNENSGNLMTRAIGDVDLCVEGMRKFTTEIFDTGVLMASYLISLL
                     VYDIKITVLSILFIPGAMVLAEKLKNVIYKYTIAFRSKSSYITGITYDAIENAMLYRA
                     SGMEAQNRVAYNTELEDLQSKAIKANIFENSMQPIYNVIAMLGVISVIYLGGTKVIDG
                     GWTIGQFSTYLIMFAAMAQKASKAAKLFNSVQKSQVSWKRIQPYLAEYRSKDRTLNID
                     VNNTRLSVEKLGFCYPSGKERIIENIGFQGEQGEIIGVTGAIASGKSTLGLALLGLYP
                     YMGSIQIDGRELQDYSEYERSRMISFMGHKPELLSDTLYNNITLGNERSAQEIGSVLR
                     DVCFDTDLKEMPDGVNTLVGNGGIRLSGGQQARIALARTLINKSKIIILDDPFSAVDM
                     ETEEKIIANLKSSYQMSLIILISHRLTIFPQITRILLIHNDLIHNDKTVESGTHEELM
                     KSSELYAEIFNLQCTAGGADDE"
     gene            complement(6707..7249)
                     /locus_tag="VF531_10380"
     CDS             complement(6707..7249)
                     /locus_tag="VF531_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEX7714420.1"
                     /translation="MEKRKWIVLNYNLPSEPSRHRVAVWRGLKKLGAVNIQQSMWILG
                     YNEPNYRALRKIAQDVESSNGEALLMETAFFDEKHEERVISLYNDIRDEEYHEFIEKC
                     EEYLGELEKEIRIEKFTFAELEEEEEEFEKLVSWHEKIATRDIFHALAGDRAKERLEQ
                     IKKAFEVYSDLVYKHNNNRF"
     gene            complement(7473..8621)
                     /locus_tag="VF531_10385"
     CDS             complement(7473..8621)
                     /locus_tag="VF531_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7714421.1"
                     /translation="MGKVFDSNYFRKIGCIVAGAVLFNWALQNIATIFALLGKTVNLI
                     NPFLIGLGIAFILNIPMQVLENRLFKKVGAKLRRLLSLFVTILLILALILLVLLAIIP
                     EIGQTFETLLAMFPGFIVKVAAWGQQLSAKFPNLGVWLSKVALDWESIGTNAFNFMKN
                     WFAGMMNSTVSAISTFFSGVLNFFLGSVFALYILFQKENLARQARKLLYASVPEAKAD
                     RFVTICALANKTFSRFITGQCTEAVILGLMFFAALNLFRFPYAMAIAVLITCTALIPV
                     FGAFIACFIGAFLILVTSPIKAFWFIALFLVLQQIEGNLIYPRVVGGSVGLPALWVMA
                     AVIIGGGTMGITGMLVSVPLCSVLYVLLRESVGKRLAEKNIPPEKTSS"
     gene            9711..>10793
                     /locus_tag="VF531_10390"
     CDS             9711..>10793
                     /locus_tag="VF531_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7714422.1"
                     /translation="MKNKPVTAKSVHEPGNKIPPSNIRAAVVIPALNPMTNLVGFVRE
                     MLGRGIQQVIVVNDGSNRSFHDIFHQLEELERCTVLTHHVNRGKGRALKTAFAYFIEH
                     FAYLDGVVTADADGQHAIEDIVKVCERLSLEQGSLILGVRNFRDKNVPKRSYMGNLMT
                     SRIFQLLYGYYLNDTQTGLRGIPASELEWLVKTNGERYDYEINMLIKARQRHLGFTTI
                     PIKTLYFNNNSGSYYSTIKDSALIFTRLVSGLILYSGSAVISGFLDILSFFLLNSVFL
                     TALSAPVRIFASTVIARVISSLFNYLMNRKIMFADTGKPANSAVRYYILCISLMMTSY
                     GLVYTVSLLWRVNESIIKLIIDSVLGI"
BASE COUNT         2983 a         2605 c         2186 g         3019 t
ORIGIN      
        1 tattagtgag acgcaccgct taatcttaca taaacttggt attatagccg gccaaaagtt
       61 tttagcccag cttttcaagg aaatagaagc caagtttgtt gaagtagtgc cggtggattg
      121 gcctgatctg gcgcaagccc gggtactgat aaaccagtat catgatcaag acctttccct
      181 aaccgctaca tgcagtgcgg ttttgatgca ccgcaataat attagtcaaa ttgccagtta
      241 tgacaggcat tttactgtat tgcaattcga gatattgcct taatggaaag aaaaactttc
      301 ctcatttatc cttccgcctt ccctcaaggg cgggcttgtt atcctttact ttacgccggg
      361 tttctccatc gatgatttca cgaattgatt gaatcaccgg ttccgagaac atatattcct
      421 taccgtattg caaattaaat tcataatcaa aatcggaagg gttatcgccc tgattatgct
      481 ggataattgt ttcgatttta tccaatgcct taacaatttt cgcttcttgg ctcttcccat
      541 cgttatattc ccgccataat gccaaaaaac cgctgcgaat actctcaggg agcggcgcaa
      601 acagcttacg taatgccgct tcctctcttt ttagtttatc ctccttggat actccaatct
      661 ttgccgagat atccccctcg tatgcttcgc ccagatcatg aaccagactc atccggacta
      721 cccggtcgcg atcaaccccc ggaaaataat ccaccaggac catggcgaac atcgccaatc
      781 tccaagaatg ctcggcaacg ctttcctgtc tgccttcctt ggtccatgcc gtcctggttg
      841 tatctttcaa cctgtcaagt tccttaatga aagcgaccaa ttgatcatat tcgttcattt
      901 atactctccc tctcccttgg tttcagttaa gttaattcgc acgcacttta gggaagtccg
      961 agtatcattt tcgtacgcaa tgatttcctt gatcactccc ccacgaaaaa cggtatattc
     1021 gcactgtttc ctcatataag acccggagtt aggccggctt cacctcaaat aaggaacggc
     1081 gcgagatttt aaatataatt atgatcaatc gtaattaccg aatgatagtt gcggttaatc
     1141 ccgtatattt tcgccgtgag cagcttaacc agttcatcat cgctccattg aaaatcaaag
     1201 ggaatcacga cgtcgaagat gagattggaa tggctcacgc cccataccac ccgaaaatca
     1261 tggatgctga tctccggaga aatctgcttg accagatttt cgactgtctc tttcaattga
     1321 ttggttttca catcattggt gacaatcgga tcaagatgga taaccatatg aatcccgcga
     1381 tcttttaaaa agtcccgttc aatattgtca acgatatcat ggcttatcat gatatcctgc
     1441 tcggccggga cctcacaatg caccgaggca aaacattgag ccgcgccgta gttgtgcacc
     1501 gtcaggtcat gtagaccaat gataccgtcg taacttaaaa tcttttgata gataccgtcc
     1561 accaattcct tggtaggcgc cgtgcccaat agcggactaa tcgtatccga aatcagtctg
     1621 actccggtaa tcataataaa cacagcaact accacaccca tgtatccgtc taaattataa
     1681 ccggtaaaac gggtgactaa cgtggcaacc aatactgccg atgtggccag aatatcgttt
     1741 tggctgtcga cagacgttgc gataagcgtg gtggacttga tgatcttccc gattttccga
     1801 tagaacatac actgccacag cttgataagg atcgcgatca ccagcgtcac aattgttatg
     1861 aggctgaact ccgctacttg aggatgcatg attttgctca ccgaactttg aatgagctgc
     1921 aaccctaaaa ccagaatgac gaaagataca atcaagccgg agatatactc catccgggca
     1981 tgtccgtacg gatgctgggc atcggccggc ttcccggata ttttaaagcc cagcagggtt
     2041 accagggagg agccggagtc cgacaggtta tttacggcat ccgcaacgat ggcgatactg
     2101 ttaaagagtg tccccacggt aattttgatc agaaagagca aaaaattcga ggcaataccc
     2161 acaacgcccg cgaacttgcc ataacgctcc cgtactttaa aattcgctat attttgatga
     2221 tctttcacga acaacctgat taataaattg gtcatgcttt cacctcgttc tatggtttaa
     2281 cccaaatccg ggactaaaat atatggacac tcttcataat caccagtaga atgacgaacc
     2341 acaggaagat aattagggcc attccaatcc ttttatactt ctcgtcaata ctcgccccac
     2401 tttcggcttg tcgcttacat tcctcccaca cacttttagg aatcatttta atggcaagga
     2461 ttactcccaa aggaataagt ataatatcat ccagcattcc caaaaccgga ataaagtcgg
     2521 gaatcaaatc gatgggacta acggcataaa tgaggataaa tagtaaaaag gctttcttat
     2581 accatggcac accggcatgc ctataggcaa gatagacgat gatgatctgc ttctttaact
     2641 gttttgcttt tgccttgatc gtttctagca cccatatcac caacccattt acgttctttg
     2701 gggaaattcc gttttacatt tcggacaggt aatgatgatt ttccctttgc ctttcggaac
     2761 ccgtaagctc tgcctgcatt tcgggcaagt ataatattta tgagtttttc tatccctgaa
     2821 acggttctgc tctttcttaa accaagaata caccggactc atccgtatgg cgaacttata
     2881 attctccata ctgcgtttcg atgtattccg cgatagcatt cgaaaaaccg ctattcccaa
     2941 gggaacataa ccaatgaatg ccaataatcg cagttgcgcc aactgagcta tcaaggtcaa
     3001 tattgccgaa aatatgatca ggaccatgga aagctggtcg ccgccatacc ttcccatcat
     3061 gaatcttctg aaccagttca ttgccattac tcctcattga tattttaggg tggtaaccgt
     3121 agttttgccg gttcattctt aaatcagctt catccctaga tcagcttcat ccctagatca
     3181 acttcatccc aagtcaactt ctcaagccga atatggctgc ggtaccaatc gtcgttttgc
     3241 aataacattt caggcgaacc tgaatttttt accttcccat tttctaaaat aactacggta
     3301 tcgctggcaa tcattgtgga tagccggtga gatattgata gtatcgtgta cgtatccccc
     3361 gctttttgca atacggacac gattttctcc tccgtaatcg aatccaggcc tgcagtgatc
     3421 tcatcaagca gcaaaatcgg gggattggta acaattgccc tggcaatcga cagaagttgc
     3481 ttttgcccct gggaaaaaag agaatcgccg ttaacctgtg tgtccagacc attttccaaa
     3541 gatgccacat aatccgcaag ccccacaaaa tcaagggcgt tttgaatttg tccgcgagtg
     3601 atgctcttgt cctgcaggct gatttgttcg gcaacagtgc ctttgatcat atggaagctt
     3661 tggtcgacaa acccgaatag tttccgtttc tcggagttcg gtatcgtata aacatctatt
     3721 ccgttgatgg taatacttcc ctttgctggt tttaatatac ccattatcaa tttaaacagg
     3781 gtcgattttc ccaccccggt tcggcccaca aaggtggctt tctcccccgg atggatccgg
     3841 aggttaatgc tttgcaagac atcggttcct tcatcataat gaaaggttag gtcatgaaat
     3901 gacacgctta cttcttcgcg ccttggaaca atagcttccg ccttcagttg actgtttttc
     3961 ggatcatctt ccggctcgtg gtaaaattca ttcaccctgt gaatcccgga gaacgcctgt
     4021 tggatatttt ggagttccat gcccagggtc tcgatgggtg caaaaaggtt ggagatcaat
     4081 tcgatcgatg cggcaaccat tcccagggat attcccaaat agctcggttg tttggaggat
     4141 aatatcacaa taaccgcaat taccgccgcc cgagttaact gaatgatggg cgaaaatatt
     4201 gagtcataga aattaacttt ttcgacggtt tgaaaatttc tcagcaataa cccggcatag
     4261 ttctcctcca tatagtcttc cttgctatag gatttgatca tccttatatt cttcagactt
     4321 tccgcaagat ggttatttac cttgcctacc aggcttcggt tatcaatttg cgctgtcagc
     4381 attctccttt gaaaaagccg ggtaatccca tagatgacag gcagcgatat caagctaacc
     4441 attccgagct tcccgctgaa cacccaaatc gagacgacaa tcccgatgat tttgaagcag
     4501 tcaatgatca ttcccacaat gccgccggta aacatggagc taacggcgtc gacatcattg
     4561 gtgaaccggg aaaccaccgt acccgattca ttggcggaaa agaatacggc gtttatcttt
     4621 tccagcttct ccatcatttt aagccggatt tccttggtga ttttttggcc taaaaccgta
     4681 agtacggctt ctttcataaa atcaaagata ccaatcagaa gtaatacagc catgtaagca
     4741 accgccaagg tacgcagtcc gtcgcgcttt cggggcacaa ggctgttgtc aatgatattc
     4801 ttcaatattt gaggaggaat caagcttagg ataacggcgc cgccaacgac aaaaaccaat
     4861 aatccaatga ttgaaatgtt ttttttgatg acattcgcta tggactcttt gaccaaacga
     4921 ttattcatca tcggcgccac ccgctgtaca ttgtagatta aagatttccg cataaagctc
     4981 cgagcttttc atcagctctt catgggtacc ggattccacc gtcttatcat tgtggatgag
     5041 gtcattgtgg atgaggagaa tccgggtgat ttgggggaaa atggtcaacc ggtgagaaat
     5101 aaggattatc aaactcattt gataactgct tttcagattg gcaataattt tttcttccgt
     5161 ctccatatca accgctgaaa acggatcatc caagatgata attttacttt tgttaatcag
     5221 cgttcttgcc agcgctattc ttgcctgctg accgccgctt aacctgatcc cgccgttgcc
     5281 aaccagcgtg tttaccccgt cgggcatctc cttcaaatcg gtatcaaaac aaacatccct
     5341 taaaacagaa ccaatctctt gcgcgctccg ctcattaccg agggtaatat tgttatacaa
     5401 ggtatcggag agcaattcgg gcttatgacc cataaaggat atcatccggc ttcgttcata
     5461 ttcggagtaa tcttgaagtt ccctgccgtc tatctggatg ctgcccatat aggggtataa
     5521 tccaagcaaa gccagtccaa gtgtagattt cccggatgct atcgcgccgg tcacgccgat
     5581 gatttcccct tgttctccct gaaaaccgat attttcaatg atccgctctt tgccgctagg
     5641 atagcaaaag ccgagctttt ctacagaaag ccttgtatta ttaacgtcaa tgtttagcgt
     5701 cctatcctta cttcggtatt cggcgagata aggttgaatc cgtttccatg agacttggga
     5761 tttttgcacg gaattaaaga gcttggccgc cttgctcgcc ttttgggcca ttgcggcaaa
     5821 catgataaga tatgtggaaa attgacctat ggtccaaccg ccgtcgataa cctttgtacc
     5881 gccgaggtag attacggata ttacccccag cattgcaatg acattgtaga tgggctgcat
     5941 ggagttttca aaaatattgg ccttaatggc cttactctgc aaatcctcca actcggtatt
     6001 atatgccacc cggttctggg cttccatgcc gcttgcccga tagagcatcg cgttttcaat
     6061 cgcatcataa gtgatacctg tgatatagct gcttttggaa cgaaaggcaa tcgtatattt
     6121 gtaaatgacg tttttcaatt tttcggccag aaccattgct cccggaataa acaaaatcga
     6181 aagcacggta atttttatat cgtacacaag cagggatatg agataggaag ccatcagcac
     6241 tccggtgtca aatatctcgg tagtaaattt ccgcatgccc tcaacgcaca gatcaacgtc
     6301 cccaatggct ctggtcatta agttcccgga attttcatta tcaagctctt ccgtactttt
     6361 atgcataata ttgttgtaaa gcatcagcct catggtagcg ctggtgttat tggcaaagcg
     6421 ccgaatataa aaacgcttaa aataacggag aaattgaatc attccaataa gggccacgta
     6481 tatcgcagcc agtttaccta cagcggacag gctgtcgccg cgcacaatgg catcaatcag
     6541 cttaccttga tagatcggtc ctaaaatgat ggagccatta aaggtcaggc caaacatcac
     6601 aatacaggcc acgacgaatt tctctttttt ccaatagttg atgatccgat cggcctgttt
     6661 cataggttct atctttaact ttgccatcac ggataactct cttttcttaa aatctgttgt
     6721 tgttatgttt ataaaccaga tcgctgtaaa cttcgaatgc ctttttgatc tgttccaggc
     6781 gctcttttgc cctatctccc gccaaggcgt gaaagatatc ccgggttgct attttttcat
     6841 gccatgagac cagcttctcg aattcctcct cttcctcttc aagctcggcg aaggtgaatt
     6901 tctcgatgcg aatctccttt tcaagctccc ccagatactc ctcacatttt tcgataaatt
     6961 cgtggtactc ctcatcccgg atatcgttat agagagaaat aactctttct tcatgttttt
     7021 catcaaaaaa agccgtttcc atcaagagtg cttccccgtt actcgattca acatcttgag
     7081 caattttccg gagggcgcgg taatttggtt cattgtaccc caaaatccac atggattgtt
     7141 ggatatttac cgcccccaat ttctttagtc ctctccatac agccactcta tgccgggatg
     7201 gctccgaagg gagattataa tttaaaacaa tccactttct tttctccata gccaaaacct
     7261 ctctgtaaca ctggttacat caattctaac acattgtaac cgatattaca acttcaaaaa
     7321 taacaagaaa ctctttaggg tcatagggat gaatatcacg gcacgaagga atataacgga
     7381 ataatccgta tgggagattt ttataggagt atcctgtggg gatcttaagg gtgtcggttc
     7441 gtctcccgtg aatgggtgaa ttgagggatc ctttacgatg atgttttttc cggtggaata
     7501 tttttctcag ccagtctttt gccgactgat tcccgcagta gcacatataa gaccgagcac
     7561 agcgggacac taaccaacat tccggtaatt cccatcgtac cgccgccaat gatcaccgcc
     7621 gccattaccc acagagcggg gagcccgacc gaaccaccaa ctacacgagg atagataaga
     7681 ttgccctcaa tctgttgcaa taccaggaat aatgcgataa accagaacgc tttaatcgga
     7741 ctcgtgacta aaatgaggaa tgccccgata aaacaggcta tgaatgcccc gaataccgga
     7801 atcaaagcgg tgcaggtgat taggacggca atagccattg cataaggaaa gcggaaaagg
     7861 ttcaaagctg caaagaacat caaccctaaa ataaccgctt ccgtgcattg cccggtgatg
     7921 aatctggaaa aagttttatt ggcaagagcg cagatagtga cgaaacgatc cgctttggcc
     7981 tccggcaccg aggcatagag caatttcctt gcctgccgag ccaaattttc cttttggaac
     8041 agaatataca gggcaaacac agaaccaaga aaaaagttta acacgccgct gaagaatgtt
     8101 gatatcgcgg agacggtaga attcatcata ccggcaaacc agtttttcat aaaattgaat
     8161 gcgtttgtcc cgatgctctc ccagtccaga gccaccttgg aaagccatac gcccagattg
     8221 ggaaatttgg cggaaagttg ttggccccat gccgccacct tgacgataaa gccgggaaac
     8281 atcgcgagca gcgtctcgaa ggtttgccca atctccggga tgatggccaa cagcaccaat
     8341 aaaatcaaag ccaatattaa taatattgtc acgaacaggc tcaagaggcg gcggagcttg
     8401 gccccgactt ttttaaacaa cctattttcc aatacctgca tcggaatatt caggataaat
     8461 gcaataccga gcccgatcaa aaaggggttg atcaggttga ctgttttccc caacaatgca
     8521 aatatggttg cgatgttttg cagggcccaa ttaaataaaa cagctcctgc aacgatacac
     8581 ccgattttcc ggaaatagtt cgaatcgaaa actttaccca cctcatttgg ccaccctgtc
     8641 aatgcactca tattaatttt agcataattt cttccaaaat atccatatct ccatccggga
     8701 ggcaaagatt gtagccgctt gtatatcgcg ccatgcggcc tcatccgttt ctaaaaacag
     8761 aacttcgata ctgaacacgc cgcttgatcc tgcatgtttt ttcatggcaa tgccggcact
     8821 gcaaatttat attgctctcc tcccaaaaac gttgcgggct cttccgataa gtagcctccc
     8881 aacgaatcaa taaaattaaa gcaatcaaaa ccaaactgcc cgagaaaata ctccggacaa
     8941 aaatcagcgg tgtaagtatc ataatggaat cccaattgaa gatgcggcaa gttacacaac
     9001 aacggttttt cataatcaaa gattgcaatg ggcaataaaa taagacacaa accaaatcgc
     9061 atacatagta gaatagggat aacagcacca aggcgctttc atttaaaatc cccgtacgat
     9121 aaagaatggc cacaacggaa tttccgccaa accatacaat aaaaactttt atcgcggccg
     9181 cgtcttcttc tttaacccac gcggcaattc ccgatttgat aaaattccct tcgcttggtg
     9241 cataggtaga cttgaattgt ttacggcaac ccatactggt tatcttttga gggaagaact
     9301 tttgcaccat cccagtcaaa agtatcagcc acaaaatatg gactggcttg acaccgttag
     9361 cgaatgaaaa accctttgaa atgattaagc tgttttgatc ccttatgtaa agccgaaccg
     9421 cagcgataaa cagcgcaatt ctaaaagcca gccttgcgca ataaagtctg aacatgttgg
     9481 gtctatgctc ctgtaatttc cgggatgttt attatatata ctatttgcta taattatttt
     9541 tgtcaataag catgcgggta tcggatgaat ctaaaaaatg ttaaaatgaa ttataaagat
     9601 gaatttatcc agtcacaaac cgatgcatca tgtacttatt atttaatgta tgatattttt
     9661 tatttacagc ctcctcccaa aaatcgaaac cgatcggaat gatgtgattg ttgaaaaaca
     9721 agcctgtaac ggctaaatcc gtgcatgaac caggcaacaa aatcccccca tccaatattc
     9781 gcgccgctgt cgtaatccca gccttaaatc ccatgacgaa tctggtcggc ttcgtccgtg
     9841 agatgctggg gcgggggata cagcaagtta ttgtggtcaa cgacggcagc aatcgctcat
     9901 ttcacgacat cttccaccag ctggaagagc ttgagcgctg cactgtgctt actcatcatg
     9961 tgaaccgtgg caagggcaga gctttgaaaa cagccttcgc atatttcatc gaacacttcg
    10021 cctatttaga tggcgtagtc accgccgatg ccgacgggca gcacgcgata gaagatattg
    10081 tcaaagtctg tgagcgtttg tcgttggagc aagggtcctt gatcttgggt gtaaggaatt
    10141 ttcgggataa gaatgtgcca aagcgaagtt atatgggtaa tctgatgacc agtcggattt
    10201 ttcaattgct ctatggctat tatctaaatg acactcaaac cgggctacgg ggtatccctg
    10261 ccagtgagct tgaatggttg gtcaaaacca atggagaacg gtatgattat gaaattaaca
    10321 tgctgatcaa agccaggcaa aggcatctcg gcttcacaac aatacccatt aaaacgctgt
    10381 atttcaataa caattccggc tcttattaca gcactatcaa ggattctgcg ctgatcttca
    10441 cgcgtctggt ctcagggctg atactgtatt cgggatccgc cgttatttcg ggatttcttg
    10501 atattcttag ctttttcttg ttgaatagtg tgtttcttac cgccttgtcc gcaccggtga
    10561 ggatttttgc cagtacggtt attgcccgtg tgatatcctc cctattcaat tatttaatga
    10621 atcgcaagat catgtttgcc gataccggca aaccggccaa ctcggcagta cgctattaca
    10681 ttttatgcat ctcgctgatg atgacctcct atggattggt ttatacggtc agcttgttat
    10741 ggagggtcaa cgaatccatt atcaaactca tcattgattc cgttttgggg att
//
LOCUS       DASVMB010000364         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_339765,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000364 DASVMB010000000
VERSION     DASVMB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339765"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VF531_10395"
     CDS             <1..812
                     /locus_tag="VF531_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714423.1"
                     /translation="YIILCGAGLLYLAPLLWLFDVSFRPMVEIFSVPPPFFQKPIWET
                     FNTYSLTAYIGAIEQQVPLAVFNSTIVAIMGIVVTLLLVSLAAYAFACMRFPGKNVLF
                     VLIIATMMLPNQTMLAPLYHIFRVIGLTDNLAGLVLLYAATAYCFWLMRQYIICIPKS
                     LMKSAQIDGANKLKIWWHIIVPLSKPAMAALAILQFRTIWNDFLVPIILMKTDTLYTL
                     PLRIQLIDSFVVNKRYDILMATGFIAALIPLIFFLLFQRQFIEGLSGGIKG"
     gene            793..1584
                     /locus_tag="VF531_10400"
     CDS             793..1584
                     /locus_tag="VF531_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714424.1"
                     /translation="MAELKDNRIGQGGVFVQRGTDELRSQSIRLIIADDMVPIREYLR
                     MILSREPDMKVIDAVGTGSKAVDRSLIVRPDVLLLDLEMETPRAGIEAIHTLAAPGSR
                     IRCVVLTHYRDEDMLFAAFEAGAADYILKDSPTAEIVEAVRMAAHDQRPIRPLVARMI
                     RDELRALRSELDVSVVTLQIIFNLSSKDLGILRLLANGKDENEIGRLQWVEASIIRNH
                     VRNLLKKFNEPSIADLINRFSRLGILGIFTHEKDIGRKTCSKTRI"
     gene            1622..>2008
                     /gene="rnpB"
                     /locus_tag="VF531_10405"
     ncRNA           1622..>2008
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF531_10405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          492 a          441 c          516 g          559 t
ORIGIN      
        1 gttatattat cctatgcgga gcgggtttgt tatacttagc cccgttatta tggttgttcg
       61 atgtgtcgtt ccgccccatg gtggagatct tttcggtgcc gccccccttt tttcaaaaac
      121 ccatctggga aacctttaat acttatagct taactgcata tatcggggct attgaacagc
      181 aagtgcccct tgcagttttc aattcgacta ttgttgcaat tatgggcatc gtcgttactt
      241 tacttttagt ctctttggct gcctatgctt ttgcctgcat gcgatttccg ggtaaaaacg
      301 ttttatttgt tttaatcatt gcgacaatga tgttgccgaa ccagacgatg ttggccccgc
      361 tttatcatat cttccgggtg attggtctca cagataattt ggcggggttg gttttacttt
      421 acgccgccac ggcctattgc ttctggttga tgcgtcagta tatcatttgt ataccgaagt
      481 ccttgatgaa aagcgcgcag attgacggcg ccaataagtt gaagatctgg tggcatatta
      541 ttgtgccgtt atccaagccg gccatggccg cgctggctat ccttcaattc cgtacgattt
      601 ggaatgattt tttagttccc atcattctaa tgaaaaccga tacattatat acgcttccgc
      661 tgcggattca gttgatcgat agttttgttg tcaataagcg ttatgatatc cttatggcca
      721 ctggattcat cgcggcattg atcccgctaa tctttttcct cctcttccag cggcaattta
      781 ttgaaggttt gagtggcgga attaaaggat aaccgtatcg ggcagggagg agtattcgtg
      841 caacggggga cggacgaact tcggtcgcaa tcaatccgat tgattatagc cgatgatatg
      901 gtgccgatcc gagagtatct tcgcatgata ttgagccgcg aaccggatat gaaagtgatc
      961 gatgccgtgg gaaccggatc caaggccgtt gatcgttctt tgattgttcg accggatgta
     1021 ctattactgg atttggagat ggaaacacca cgggccggga tagaagctat tcatactctg
     1081 gcggctccgg gctcacgcat ccggtgtgtg gtattgactc actatcgtga tgaagacatg
     1141 ttgttcgcag cctttgaggc aggggcggcg gattatatac tcaaagactc gccgacagcc
     1201 gaaattgttg aggctgtaag aatggcggca cacgatcagc gaccgatccg tcccctggtg
     1261 gcacggatga ttcgggatga actccgggct ctccggtcgg aacttgacgt ctctgttgtc
     1321 actttacaaa ttattttcaa cttgtcctca aaggacttgg gaatactgcg gttattggca
     1381 aatgggaaag atgaaaacga aattggacga ctgcaatggg ttgaagcatc gattatccga
     1441 aaccatgtgc ggaatttgct taagaaattc aacgaaccct cgatagccga tttgattaac
     1501 cgattcagtc gcttgggaat tttaggcatt ttcacccacg agaaggacat tggcaggaaa
     1561 acatgctcaa agacgagaat ttgacatcct ggttttgata tgttaaactg atgaaactaa
     1621 ataactgctt cgggtaaccg cgggccgcaa ggtccgagga aagtccgagc tccatagggt
     1681 aggatgctgg ataacgtcca gggggggcga ccccacggaa agtgccacag aaaaggaatc
     1741 ggcatcttcg gataacgccg aagctgttag agtttattct aaactctacg ctttggccat
     1801 gtgctgaagg taccagagct gaaaaggtgc ggtaagagcg caccagcagt caaggaattg
     1861 gctggctcgg caaaccccat ccggagcaag gccaaatagg gaagagataa agtgacccgc
     1921 ttttcttccg ggttggccgc tagagatacc gggcaaccgg tatcgtagat aaatggttac
     1981 cgttattcac ccttacggat gactaata
//
LOCUS       DASVMB010000365         7365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_635158,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000365 DASVMB010000000
VERSION     DASVMB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635158"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VF531_10410"
     CDS             complement(<1..627)
                     /locus_tag="VF531_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7714425.1"
                     /translation="MPRKSYVTSMPDQVGAFLLASKIIAAHDGNIVRVSYNKAVDLHT
                     LFIDVVASAESLAAITRELENVGYLQDKILETKVIVVSIKIPDRPGAVLPVLQILERY
                     DINISYINSCENKTFHQNFKMGLLIENPEIIKMLLHEISELYQIDILEYDDSETSLDN
                     TIFYIRLANEMQKLLGLSTEKTMEFISESNRILQLLQDKGENPNKVFDY"
     gene            complement(894..2627)
                     /locus_tag="VF531_10415"
     CDS             complement(894..2627)
                     /locus_tag="VF531_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714426.1"
                     /translation="MNSKLKKFLSYYRPYLGLFTADLGCAFTASGISLAFPLIIRYIT
                     NDIFRAGATVNMGSVLKPACAMVVMVIIEYFCNYFITTFGHFMGAQMEYDLRNEIFDH
                     LQKLSFNYYDNQKTGQIMSRITNDLFEITELYHHGPEDLLISLTKLAGAFIILYGINL
                     RLTLVIFAFIPPMIGFAYYYNREMRKTFRKNREKIAAINAGIEESISGVRVVKSFTNE
                     DLEMEKFRESNHSYVEAKKNSYRYMGKYNSGVNAFSSLIYVVLVVSAALFISGGQIKP
                     IDLLTFLLYINVFLEPIKKLINFGEQFQNGISGFDRFYEILSIAPDIADTKGAVSLTK
                     VKGDIEFKNVSFHYRESSAEVLSRINLNVQAGEYVALVGPSGVGKTTLCSLIPRFYEV
                     SAGSILLDGRDIREIKLKSLRRKIGIVQQDVYLFAGSVMENIRYGNPEAGIEEVVAAA
                     KNANAHDFIMELPNGYETDIGQRGVKLSGGQKQRLSIARVFLKNPPVLIFDEATSALD
                     NESEKVVQDSLETLARGRTTFVIAHRLSTIRNARRILVLTAEGIAEEGNHESLMARNG
                     IYAQLYKMQFK"
     gene            2910..3953
                     /locus_tag="VF531_10420"
     CDS             2910..3953
                     /locus_tag="VF531_10420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7714427.1"
                     /translation="MLTELSNDSFTGLPNLFGLVESATTGAFTISGTIVAADIAYLEQ
                     VNQNYGRRAGDGCVRALARLLRDETLRSVGSRPAAFRIGGDEFVLVLPGYTQEEAQLI
                     AYNLGASFRKKAIADGILGVNLHTAVVTYEEKGLTAATLVKAAYLALADCRYTAEIPA
                     KLPKWVEGLIDNMAERIYETLGLLRETYSLALSDEISGLPNHRAAELYLEEMFTEYRQ
                     SNFPFAILFIDGDNLRQYNDLGYEQGNRMIHELGLILAGSLRQGDRLTRWLSGDEFMA
                     LIPGADRKLALQVAERIRLAVEAATRNWPIPITVSIGVAVCPEDAVTAPELLEKAENA
                     ITAAKKTGKNQVV"
     gene            4050..4916
                     /locus_tag="VF531_10425"
     CDS             4050..4916
                     /locus_tag="VF531_10425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7714428.1"
                     /translation="MWLADNWEDYELLDTGSGERLERWGKYILRRPDPQVIWPFINSG
                     APWDRADGHYHRSSSGGGNWEIRGQLPERWVVRYKSLSFYIHPTGFKHTGIFPEQAVN
                     WDWLSQKIRSRSGSPVRILNLFAYTGAATVAAAAAGAEVCHVDAAKGMVTWAKENLVL
                     SGLGDRPARFLVDDVIKFVAREQRRGRQYEAVIMDPPSFGRGPGGEVWKIEERLYDLV
                     AACTAILSPQPLFFLINSYTTGLAPSVLGNVLALTVGKRFGGRLSADEIGLPVSASGI
                     FLPCGASGRWES"
     gene            4919..5644
                     /locus_tag="VF531_10430"
     CDS             4919..5644
                     /locus_tag="VF531_10430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7714429.1"
                     /translation="MTESPGTCPVTTVLPNRKISILYEDNHLLVVEKPVNLPTQADES
                     GDPDLLSLLKSDLKLRYGKPGNVFLGLVHRLDRPVGGVMVFAKTSKAAARLAEQVRHR
                     ELQKKYLAVLHGVPEPRRGRLQHYLVKDHMANMVTALTTEAPQSKLAVLDYEILAERE
                     GLALAEVHLETGRSHQIRVQFAAGGHPLYGDQKYGAGLNRIGQQLALWSVELGFKHPT
                     RGEPLIFRSRPPQVFPWSLFPLD"
     gene            5681..5757
                     /locus_tag="VF531_10435"
     tRNA            5681..5757
                     /locus_tag="VF531_10435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5715..5717,aa:Val,seq:cac)
     gene            5778..5855
                     /locus_tag="VF531_10440"
     tRNA            5778..5855
                     /locus_tag="VF531_10440"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5813..5815,aa:Asp,seq:gtc)
     gene            5875..5950
                     /locus_tag="VF531_10445"
     tRNA            5875..5950
                     /locus_tag="VF531_10445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5908..5910,aa:Phe,seq:gaa)
     gene            6244..6525
                     /locus_tag="VF531_10450"
     CDS             6244..6525
                     /locus_tag="VF531_10450"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX7714430.1"
                     /translation="MFIRVIRLTLLIPGAQTIKDKRQVLQSLMTKLRAKFNISLAESG
                     YQDLWQRSELGIAIVGCTQSYLDGVQQELIEMIEDNYPVEITDTTIADY"
     gene            7044..>7365
                     /locus_tag="VF531_10455"
     CDS             7044..>7365
                     /locus_tag="VF531_10455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714431.1"
                     /translation="MFTYRKARMRDAENLHALINDFAGRGLMLPRSLNTIYESLREFI
                     VIESGTELAGFGALHIIWEDLAEIRALAIRSEYQRQGIGTALIGELLDEARNLGIHQV
                     FALTY"
BASE COUNT         1786 a         1751 c         1911 g         1917 t
ORIGIN      
        1 atagtcaaag actttattcg ggttctctcc tttatcctgt aacagctgca aaatccggtt
       61 ggactcggag atgaactcca tggtcttttc ggtgctcaat cccagaagtt tttgcatttc
      121 gttggcgagg cgaatataaa agatcgtatt atccaggctt gtttcagaat catcatactc
      181 gagaatatca atctggtaaa gttcgctgat ctcgtggagc aacattttaa tgatctcagg
      241 attctctatc aacagcccca ttttaaaatt ctgatggaag gttttatttt cgcatgaatt
      301 gatataagaa atattaatgt cataccgttc caggatttgc aacacaggca agacagcccc
      361 cggcctgtcg gggattttga tcgaaacgac aattactttg gtttccaaaa ttttatcctg
      421 aagatagccg acattttcca actccctggt aatggcggca aggctttcag cactggccac
      481 cacatcaata aacagcgtgt gcaggtcaac cgctttatta tagctcaccc ggacgatatt
      541 gccgtcatgt gcagcgatga tcttcgatgc caacagaaat gcgccgacct gatccggcat
      601 gctggtgaca taggattttc ggggcatggt gaagcctcct atacggtaaa caagatgaaa
      661 gcgaaggcga ctcatccctt gccctcacat ccgaggatgg acgcgaactc tcccgcgact
      721 cacccccggc ccctctctcc cgcaagcggg aaagagggga gaaaactgat tcagggtggt
      781 gataaaaaag gctgcgaagg atcaaggtta ctctctctgg cgcttgccgc cagagagagc
      841 gagagtgagt cgtaggcgcg aaagcgactc acccctctgc ctttcggagt cgctcactta
      901 aactgcattt tatataattg ggcgtatatc ccattcctgg ccatcagact ttcatgatta
      961 ccctcctcgg cgatgccctc cgcggtgaga accaggattc tccgcgcgtt gcggatggtc
     1021 gagagccggt gggcaatgac gaatgtagtc ctgccccggg ccagggtttc cagggagtcc
     1081 tgaaccactt tctcgctctc gttatccagg gccgaggtgg cttcatcaaa gatcaggacc
     1141 ggaggatttt ttaaaaatac ccgggcgata ctgagacgtt gtttctgtcc cccggagagc
     1201 ttgacgcccc gctggccgat atcggtctcg tagccgttgg gcaactccat aataaaatca
     1261 tgggcattgg cgttcttggc cgcggcaacg acttcctcga tgccggcctc cgggttgccg
     1321 tagcgaatat tctccatgac cgatccggca aaaagataga cgtcctgttg aacaatgccg
     1381 atctttctgc gcagggactt gagtttgatc tcccggatgt cccttccgtc aagcaggata
     1441 ctgcccgcgg aaacctcgta gaaccgtgga atcaggctac acagggtggt ttttcccact
     1501 ccggaaggtc ccaccagtgc aacatactcc ccggcttgta cattcagatt aatcctggat
     1561 aaaacttcgg cggaactctc gcgataatgg aaggagacat tcttaaattc gatatcccct
     1621 tttaccttgg tcagggagac agcacccttg gtatcggcga tatcgggggc gatggacaag
     1681 atctcataaa agcggtcaaa accggagatc ccgttttgaa actgctcgcc gaagttgatc
     1741 agttttttga tgggctccag aaatacattg atataaagta aaaatgtcag cagatcgatg
     1801 ggtttgattt gccctccgga gatgaagagc gccgccgata cgaccagcac cacgtagatt
     1861 aaggaactga atgcattgac gccgctattg tatttgccca tatagcggta gctgttcttt
     1921 tttgcctcca cataactatg gttactttcc cggaattttt ccatctccaa atcttcgttg
     1981 gtgaatgatt tcaccacccg aaccccggag atgctctcct cgattccggc gttgatcgcc
     2041 gcaatctttt cccggttctt ccgaaaagtc ttgcgcatct cacggttgta atagtaggcg
     2101 aaaccgatca tcggagggat aaaggcgaaa atgaccagtg tcaatcggag gttaatcccg
     2161 tagagaataa tgaaggcccc tgccagcttt gtcagggaga ttagcagatc ttccggacca
     2221 tggtggtata attcggtgat ctcaaataaa tcgttggtaa tccgggacat gatttggccg
     2281 gtcttttggt tgtcgtaata attgaacgag agtttctgca ggtggtcgaa gatctcgttc
     2341 cggagatcgt attccatctg cgcccccata aagtgcccga aggtagtgat gaaataatta
     2401 cagaaatact cgataatgac catcactacc atggcgcaag ccggttttaa gacagatccc
     2461 atattcacgg ttgctccggc ccggaaaata tcattggtga tataccggat aattaatggg
     2521 aaggccaaac taattcctga ggcagtaaag gcacagccca gatcggcagt gaataacccc
     2581 agataaggcc ggtaatagga taggaacttt tttagctttg aattcatcat ggattccttc
     2641 aatcaatttg tattcatgta caatcttcgt taactctgat ttcttacaag acatgccgtt
     2701 tatgacggtt tgtgtataga ttatcatgga aacaatgtcc cggcaagcgg cagaaaaatt
     2761 attgggttaa gatatcatcg ggcgggaacg ccaagttttt atattaatga acaggcgatt
     2821 cggaatgaag tccatgcagg actgcaatat tttaaatatg atataataaa tacgaatggg
     2881 caattttaga tttttggctg gggtgtttga tgttaactga actaagcaat gattccttta
     2941 ccggcctgcc gaatttattt ggcttggtcg agtccgccac caccggagca tttacaatct
     3001 ccggcaccat tgtagcggcg gatattgcat acttggaaca ggtcaatcag aattacggcc
     3061 gtagggccgg agatggttgc gttcgggcgt tagcccgtct gttacgggac gaaactctgc
     3121 gatcggtggg ttcgcgtccg gctgccttcc ggatcggcgg cgatgagttc gtcttggtct
     3181 taccgggata cacccaagag gaagcccagt tgatcgccta taatctgggt gccagctttc
     3241 ggaagaaagc tatcgccgat ggcatcctgg gggttaatct ccatacggcg gtggtaacct
     3301 acgaagagaa agggttaacc gctgccacac tggtgaaggc ggcctacttg gctttggccg
     3361 attgccgcta caccgctgag atcccggcaa aactccccaa atgggttgag ggtttgatcg
     3421 acaatatggc ggaacggatt tatgagacgc tagggctgct gcgggagact tacagtttgg
     3481 cattaagcga cgaaatctcc ggtttgccca atcatcgcgc cgccgaactc tatctcgaag
     3541 agatgttcac cgagtatagg cagagtaatt ttccttttgc catattgttt atagacggcg
     3601 ataatttgag acaatataac gacttgggtt atgagcaggg taaccggatg atccatgaac
     3661 tcggcctgat acttgccgga tccctgcgcc agggggatcg actgacccgt tggttatcgg
     3721 gggatgagtt catggccttg attcccggcg ccgataggaa acttgcctta caggtagccg
     3781 agaggattcg gttggcggtg gaggcagcca cccggaattg gccgattccg atcactgtgt
     3841 ccatcggcgt agccgtttgt cccgaggatg cggtgaccgc accggagttg cttgagaagg
     3901 ccgaaaatgc cattaccgcc gcaaagaaga ccggaaaaaa tcaggtggtt tgacaagtag
     3961 ttacatgcgg cgcgtggcgg agagaccggg gaatctttga gttgaactta agttccatcg
     4021 aaacccctga acatggagtt gatgacccga tgtggctggc cgataattgg gaggattatg
     4081 aattactgga taccggttcg ggggaacgcc ttgaacgttg gggaaagtat atcttacgca
     4141 ggccggatcc ccaagtaatc tggccgttca tcaattccgg cgcaccctgg gaccgggcgg
     4201 acggacatta ccatcgcagc agcagcggtg ggggtaattg ggagattcgc ggtcaattgc
     4261 cggaacggtg ggtggtacgt tacaaatcac ttagtttcta catccacccg actggtttta
     4321 aacataccgg catctttccc gaacaggccg ttaattggga ctggctgtcg caaaagatcc
     4381 gttcccgttc cggatcgccg gtgcggattc ttaatctatt cgcttatacc ggggcagcta
     4441 ctgtcgctgc cgccgctgcc ggggccgagg tttgtcatgt tgatgcggcc aaaggcatgg
     4501 tgacctgggc caaagagaac ctggtcctct cggggctcgg tgaccgaccg gcccgttttt
     4561 tggtggatga tgtaataaag tttgtcgcca gagagcaacg tcggggcagg caatacgagg
     4621 cagtgattat ggatccgcct tccttcggcc gcgggccggg cggagaggtc tggaagatcg
     4681 aggaacggct ttatgatctg gttgcggctt gcacagccat tctctccccg caaccgttat
     4741 tctttttaat taactcctac actaccggcc tggcgccgtc ggtactcggt aatgttttgg
     4801 ctcttaccgt cggtaaacgt tttggagggc gtctctccgc tgacgagatc ggtttgccgg
     4861 tttccgcctc ggggatattt ttaccttgcg gcgcttcggg ccgttgggag agttgaaaat
     4921 gaccgaatcg cccggaacct gtccggttac aaccgtttta ccgaatcgaa agatatcgat
     4981 tctttatgag gataaccatc tgttggtggt ggagaaaccg gtcaatctgc ctactcaagc
     5041 cgatgagtcg ggcgatccgg accttcttag tttattaaaa tccgatttga aactccgcta
     5101 cggcaaacca ggcaatgtct ttcttgggct ggtgcacagg ctggaccgtc cggtcggtgg
     5161 ggtcatggtc ttcgccaaaa cctcaaaggc tgcggccaga ttagcggaac aggttcggca
     5221 ccgcgaactc caaaaaaaat atctggctgt actccatggg gtaccggaac ctcgtcgcgg
     5281 ccgccttcaa cattatctgg tcaaggatca tatggccaac atggtaacag ccttgactac
     5341 agaggctccc cagtccaaat tggctgtact ggattatgag atattggcgg aacgggaggg
     5401 gctggcgttg gctgaagttc atctcgaaac cggccgttct caccagatcc gggttcaatt
     5461 tgccgccggc ggtcatccct tatacggaga tcaaaaatat ggagccggct taaaccggat
     5521 cggccagcag ctggctttat ggtccgttga gcttggtttc aagcatccaa cccggggtga
     5581 accgctgatt tttcgttccc ggccgcccca agtttttccg tggagtttat ttccccttga
     5641 ctaagttata ggggaatgtt ataatttttt tgcgtttgaa ggtcgcgtag ctcagtcggt
     5701 tagagcgcag cgttcacatc gctgaggtca ctggttcgag cccagtcgcg accaccattg
     5761 atttaagcgg aacatatgga gccatggtgt agttggttta acatgccagc ctgtcacgct
     5821 ggagatcacg ggttcgagtc ccgttggctc cgccatttta ttttctaaag caatgccacg
     5881 atagctcagt cggtagagca ggggactgaa aatccccgtg tcgctggttc gattccggct
     5941 cgtggcacca ttgaatatca agtatccctc ggttttatgc cgagggtttt ttatttctta
     6001 taaccgtgtc ttttaacgtg ggtttatagt accgatatcg gacaaaagct ttgtccaagg
     6061 gactaatgtg agtggggaca ttaacgaact gttttatcgg gaaactttgc taactccatg
     6121 ttcctatttt acaactatat catttttcac tataataatg atgaaatacc ttttatcttt
     6181 gcgctgtcga gtatagacaa atgtagcaat tcgcttcgcg gaacaaattc ttggagaatg
     6241 tacatgttta tccgtgttat tcgtttgacc ttactaatac ctggtgccca aaccatcaaa
     6301 gataaacgcc aggttctaca gagtttgatg accaaactcc gggcgaaatt taatatttca
     6361 ctggccgagt ccggatatca agatctctgg caacgctcgg aactgggaat tgcaatagtc
     6421 ggttgcactc agagttattt ggacggtgtt caacaggagt taatagagat gattgaagat
     6481 aattatccgg tggagatcac cgatacaacg attgccgatt actgatacct gcttcttact
     6541 ctccctaaag ggtaaggtaa tcgctgttat ctttacgctt caaccctcgg tgaccaatcc
     6601 ttgactacac atacccgctg aatgcataac aatttaatta tcccaataat aattttataa
     6661 attgataaca aagctcaaag gctactcttc cattagctgc agagctcagg gttaggggct
     6721 gtctcaaaag tccgatttat cattccgtcc acccggggtg cgcgtcccat gggacgcgca
     6781 ccccgggcta agttaaaaac cattctaaag cccgttaaaa cgggctggga agaactggat
     6841 gtattaccca gccccattca tggggcttga gatgattttt ccgggcgtag aaaggctcct
     6901 aaagggcctg gtgaggataa ggacgattat ccattgtttg cttttgagac agcccccaag
     6961 agaaaggtgt tggcataaat tgaaaaagta tgttatattt ataaggagat atttaataat
     7021 tattcaagaa ggctggcaat aacatgttta cttatcggaa agcccggatg cgggatgcgg
     7081 agaaccttca tgctttaatc aacgattttg ccgggcgggg tttaatgctc ccccgttcct
     7141 tgaataccat ctatgaaagc cttcgggaat ttattgtcat tgagagtgga acggaattgg
     7201 ccggtttcgg tgccctccat atcatctggg aggacctggc cgagattcgt gccctggcga
     7261 tccgctccga gtaccaacgg caaggaatcg gaaccgcgtt gatcggggag ctattggatg
     7321 aggcgcggaa cctcgggatt catcaggtgt ttgcccttac gtatc
//
LOCUS       DASVMB010000366         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_413690,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000366 DASVMB010000000
VERSION     DASVMB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413690"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF531_10460"
     CDS             <1..618
                     /locus_tag="VF531_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX7714432.1"
                     /translation="LTVIGQAGTLVVPTLTGKREDSPNCPPVFDVRKTPCWTGIIPET
                     VRNMDGVTRSLHPTHSVAALGSRKLAMTSGHEDSASPCDAKSPFFKNAVTGGYILLIG
                     VDQESNTSVHSCEEIAEVPYHLQRDVSRIYITGYEEERILVVNRLHDWGKPPTNFNKL
                     DELYKNLGMMTMGKIGSAEVRLIKSAAMFDFTIDLLKKDPLFLLV"
     gene            654..1469
                     /locus_tag="VF531_10465"
     CDS             654..1469
                     /locus_tag="VF531_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7714433.1"
                     /translation="MILVLIDALSFQAAHSRMSYMQHLVENRIAALYQVQTELPTLSR
                     PLYEVILTGTKACQNGVTHNFIIRRSHEVSIFELAKSQGLKTAAAAYSWISELYNRAP
                     FEPYNDREQTDIRANIQYGKFYYDDFYPDSHLFTDGEILRRRYDPDLLLIHPMGVDLA
                     GHQNGGESREYLSKTQDIDNLLADLIPAWLDLDYQIIVTADHGMNANGNHGGLESSER
                     IVPLWTLGSCFEPTDSQGLIPQLGIAPAICKILNVDKSARMIDYVFPGFIRSF"
     gene            1505..1804
                     /locus_tag="VF531_10470"
     CDS             1505..1804
                     /locus_tag="VF531_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HEX7714434.1"
                     /translation="MNIKYKGDLMESFTYAPKSVCSNRIRVLFDPETRCLVKVTFEGG
                     CEGNLTGIGRLIEGMPIQEVIRRLKGIDCDGKGTSCPDQLARALERHFMIDESTK"
     misc_feature    1826..1952
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1976..2506
                     /gene="infC"
                     /locus_tag="VF531_10475"
     CDS             1976..2506
                     /gene="infC"
                     /locus_tag="VF531_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7714435.1"
                     /translation="MSNELRINEEIRAKEIRVVSSDGEQLGILSVKDAIKLAQEKELD
                     LVEVAPAAKPPVCRIMDYGKYRYEQSKREREARKKQRVIEVKEVRMTPKIEAHDFQVK
                     VKAAQKFLKDGDKVKAIIRFRGREIVHAELGKTLLMQLYDTVRDSAVIEREPKIEGKS
                     MIMIIAGKNERTTAAE"
     gene            2598..2792
                     /gene="rpmI"
                     /locus_tag="VF531_10480"
     CDS             2598..2792
                     /gene="rpmI"
                     /locus_tag="VF531_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7714436.1"
                     /translation="MPKMKTHRGAAKRFKVTGSGKIKKSFAFRSHLLESKPPKRKRNL
                     RKSQVISAGDTRRVKRMLGL"
     gene            2814..3167
                     /gene="rplT"
                     /locus_tag="VF531_10485"
     CDS             2814..3167
                     /gene="rplT"
                     /locus_tag="VF531_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7714437.1"
                     /translation="MPRAKGGFTTRHRHKKVLKFAKGYRGSKSRIFRPANAQVLKSLA
                     YALRDRRAKKRDFRKLWITRINAAARMNGMSYSTFINGLKKAGVEVNRKVLADLAVHD
                     DQAFKDLIGVAKSGK"
BASE COUNT          901 a          740 c          820 g          823 t
ORIGIN      
        1 cttacggtaa tcggtcaagc cggaaccctg gttgtaccga ccttgaccgg gaaaagagag
       61 gattctccca attgcccgcc ggtatttgat gttcgtaaaa ctccttgttg gacggggatt
      121 attccggaaa cagtccggaa catggatggt gtcacccgct cattacaccc cacccactcg
      181 gtggcagcct tgggttcaag aaagctggcg atgacttcgg gacatgagga ttcagcctca
      241 ccctgtgatg ccaagagccc cttcttcaaa aatgccgtaa ctggcggtta tatcctgtta
      301 atcggagtcg accaggaaag caatacttcc gtccattcct gtgaagaaat tgcggaggtc
      361 ccctatcatc tgcagcggga tgtttcacgg atctacatta caggttacga ggaagagcgg
      421 atactcgttg taaacaggct gcacgattgg ggtaagccac cgaccaattt taacaagctt
      481 gatgaattgt ataaaaacct cggaatgatg acgatgggga agatcggctc cgccgaagtc
      541 agattgatca aatccgccgc aatgtttgat tttacgattg atcttttaaa aaaagatccc
      601 ctgtttttac tggtttgaga aataaaaaag gttgtgagat cacgaagcgc aaattgatat
      661 tggtcttaat cgatgcctta agtttccagg ctgctcattc ccggatgagt tacatgcaac
      721 atctggttga aaatcgaatc gccgcactct atcaggtcca aactgaacta cccacgcttt
      781 cccggccgct ttatgaggtt attttgaccg ggaccaaggc atgccaaaac ggtgtcactc
      841 ataacttcat tatccgacgt tcccatgagg taagcatctt cgaacttgcc aaatcacaag
      901 gactcaaaac cgcggccgca gcttactctt ggatcagcga gctttataac agggcgcctt
      961 ttgaacccta taatgatcgc gaacaaacgg atattcgggc caatattcaa tacggaaagt
     1021 tttactacga tgatttctac ccggacagcc atttgtttac cgacggtgag attttgcgaa
     1081 gaagatacga tccggatctg ctcttgattc atcccatggg tgtggatcta gccggccatc
     1141 aaaacggcgg tgaatcccgg gaatacttat ccaagacgca ggatatcgat aatttgttgg
     1201 cagatctaat cccggcttgg ctggatttag attatcagat catcgttact gcggatcacg
     1261 gtatgaacgc taacggcaat cacggtggac tggagtcatc ggagcgcata gtaccccttt
     1321 ggactctagg cagctgcttt gagcctactg attcgcaagg actcattcct cagttgggaa
     1381 ttgccccggc tatctgcaaa attttaaatg ttgacaagtc agcccggatg atcgattatg
     1441 ttttccccgg atttatccga tcattttgaa tcaggatgag gtataattaa gggacaagct
     1501 cctgatgaat atcaaatata aaggtgattt aatggaaagt tttacttacg cacccaaatc
     1561 cgtctgttcc aatcgaattc gggtattgtt tgatccggaa acccggtgcc tcgtaaaggt
     1621 gacgtttgaa ggcggctgcg aggggaatct gaccggaatc ggccggctta tcgaaggaat
     1681 gccaatccaa gaggtgattc ggcgtttgaa agggattgat tgcgacggga aggggacttc
     1741 ttgtccggat caattggctc gggccttgga acggcatttt atgattgacg aatccacgaa
     1801 gtaatggtat actgtggata ataaattagt caagcagaag ccatccgctt ctcacctaag
     1861 gccttaggcc gttgggttta taccggcaga agtgtgaatt tctgttttgg atcgattaag
     1921 cgggtgcttc acacctgctt tttaatttat ttagactatt tggaggtggg tgcttattag
     1981 taacgagttg cgaatcaacg aggagatccg ggctaaagag attcgggttg tcagctctga
     2041 cggagaacag ttagggattc tgtcggtcaa ggatgccatt aaactggcgc aggagaagga
     2101 gctcgatctg gtggaagtgg ctcctgccgc gaagcctccg gtttgtcgga tcatggatta
     2161 cgggaaatac cgttacgaac agagcaagcg ggaacgtgaa gcccgtaaaa aacagcgcgt
     2221 cattgaagtt aaagaggtcc gcatgactcc taaaatcgaa gctcacgatt tccaggtcaa
     2281 agtgaaggct gcccagaagt ttctcaagga cggggacaag gtcaaagcga tcatccgttt
     2341 ccgaggccgg gagattgtcc atgctgaatt gggcaaaaca ctcctgatgc aactctatga
     2401 tactgtgcgg gactccgcag tgatcgaacg cgaacccaag atcgaaggca agagcatgat
     2461 tatgattatt gccggaaaga atgagagaac cacagcagcg gaatgaattg gtttaatttc
     2521 ccgtgcacgc acgggaaaaa ccatcccgat ctgtcttcgc tttgtttaca tttttgtcag
     2581 agaggagtaa ttaattcatg cctaaaatga aaactcaccg cggtgccgcc aaacgtttca
     2641 aggtaaccgg cagcggtaag atcaagaaga gttttgcctt ccgcagccat ctgctggaat
     2701 ctaaaccccc gaaacgcaaa cgtaacctgc gtaaatcgca agtcatcagc gccggcgata
     2761 cccggagagt taaacggatg ttgggtttat aattttgaca ggaggaaata acgatgccta
     2821 gagccaaagg cggatttacc acccgccatc gtcataaaaa agttttaaaa tttgctaaag
     2881 gttatcgcgg ttccaagagc cggatcttta gaccggccaa cgcccaagtt ttaaaatcgt
     2941 tggcttatgc tttacgagac cgccgcgcca aaaaacgcga tttccggaag ctctggatta
     3001 cccggatcaa tgccgccgcg cggatgaacg ggatgtccta cagcactttc attaacggtc
     3061 ttaaaaaagc cggagtcgaa gtcaaccgca aggtcttggc cgatttggcg gtccatgacg
     3121 atcaggcctt taaggatctg atcggagtcg ccaagtccgg gaagtaaaaa aatttttaag
     3181 cagtttaaag gctgtctcaa aaggtttcaa acctatgaga cagccttttg tgttccagtc
     3241 attcctttca ttgcttcgaa tacagataaa gaataaaacc ataa
//
LOCUS       DASVMB010000367        11086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_561408,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000367 DASVMB010000000
VERSION     DASVMB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561408"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="dctP"
                     /locus_tag="VF531_10490"
     CDS             <1..665
                     /gene="dctP"
                     /locus_tag="VF531_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7714438.1"
                     /translation="LPYLFASSDQAYQAWDGEPGQAILKQFQNPGLIGVCYWDNGLRS
                     LTTQNRPVEKPEDLAGLQIRVMENPVYLEFFNFLHAKPTPLPWEEVIPAFEQGIIEAE
                     ENSVPIIYWNHLQKYQRYLILTQHCYSPFIVLTGPSLRRKLSGSDYEWVLALFRNERF
                     KQRQLIQEQTTRYLKILQDGGMRILTPDVKRFRELGREFSRNQLNHFSPQFVDYFKGY
                     LQ"
     gene            662..2710
                     /locus_tag="VF531_10495"
     CDS             662..2710
                     /locus_tag="VF531_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7714439.1"
                     /translation="MRTPRISLKLFRILGILVLILIVAHALLYQQMTRYNMTNQLFLR
                     GTELAYPLWQKVNQVLELGGNSESLYGLSYSCSQIKKTSPDLREVAVVDEQGRILAHD
                     QIKLVGTTVASTPRSSGEKMIFRETRRSLDTYLPVYHSDRLPIFVKISLSKRLMYQQI
                     RRALMTSLIFGAIAISLFMVTLFFLTNSLLGMRITRLLEGFTDLSKGCLTVRLTNPGP
                     TGSKPLRGRDELDLLMESFDTMANRLEKSDFKRRKQEQQLSYLATHDPLTGLPNRRLL
                     EYEMKKVISQHQPAKWPAFLLMDLDNFKFVNDTMGHAAGDQVLIKLTAMIKDMTLGKD
                     LLSRFGGDEFALLLTDCTDPGEAQCVAERICQAVAEYRFVFEKNSFHLGLSIGIVMID
                     GMNTDPGVILSQADTAMYSAKRQGRNRVQVYQPDDTMIRLSETSNWASQIKDALEEDR
                     LQLFYQPVVHLTDSRIDHYEALVRLRNPNGDLIAPQVFIPAAEQFGLMPQLDRWVVKH
                     VIRALQENPEIRIFMNLSSFGLADQSFLATIENALVDGGIEPKRLGFEITETALVQDL
                     HRAQRWIESLKILGCRFALDDFGAGFNSFLYLRHLPIDQLKLDGLFIKNLGREPIQKP
                     LVQAMHQLAGALGIETVAECVEDAESLEILKEIGVSFGQGFYLGVPVSVFDLNPLPKA
                     "
     gene            2749..4071
                     /locus_tag="VF531_10500"
     CDS             2749..4071
                     /locus_tag="VF531_10500"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEX7714440.1"
                     /translation="MRVYDLIWKKREGGELSSVELGFLVDGFVKGEIPDYQMSAWCMA
                     VYFRGMTFEECTALTLAMANSGETVDLSSLPGIKVDKHSTGGVGDKTSLVLLPLVAAA
                     GIPVAKMSGRGLGHTGGTIDKLESIPGFRTSLTQEEFMEQVRRCGIALIGQTGNLVPA
                     DKKLYALRDLTATVESIPLIASSIVSKKLAAGADAVVLDVKFGDGAFMHDYDQALRLA
                     QTMVEIGNRAGRRFRAILSNMDQPLGWAIGNSLEVAEAIQTLKGEGPSDLTELCIVLG
                     GTMVQMAGLAATKEAGERLVRELLHSGKGLAKLAELIKAQGGNPAVLDDPALLPHAAV
                     MESVKAPADGWVAGITCRELGLATMRLGAGRTKLGEAIRPEAGLKVFAKVGDFVKAGT
                     VIAEVHAVTPGEAASGKSDVLRCFTFSQKEIPHPELIRGTVGNGEKEE"
     gene            complement(4262..4338)
                     /locus_tag="VF531_10505"
     tRNA            complement(4262..4338)
                     /locus_tag="VF531_10505"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4302..4304),aa:Pro,seq:cgg)
     gene            4447..6069
                     /locus_tag="VF531_10510"
     CDS             4447..6069
                     /locus_tag="VF531_10510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7714441.1"
                     /translation="MRLTFCGAAGTVTGSCYLVETEQVRFLVDCGMFQGTKEIRDLNQ
                     HAFLFNPGALDFILLTHAHVDHAGLIPKLWKLGYKKPVYATKATVALSQIVLPDSGHI
                     QEMEAEWRNRKHSRTGEPMQEPLYDADDAVQSLKLFRPQNYNQEFEPAPGIKVRFRDA
                     GHILGSALIELYITESGSTTKLVFSGDLGQKGQPIIADPTIIQSADYIVIESTYGNRF
                     HEDQAQKVELLKNLIKESIHSGGNLVIPAFAIGRTQDLLYHLRELFNQGALPRIPVYI
                     DSPMAISVTEIYRDNPDCYDEDTLRLFAMHQSPFEFPNLSFIRTAEESKRLNETAKGA
                     IIIAASGMCEAGRILHHLKHNLWRPEAHILFVGYQAEGTLGRRLLEGAKVVKIMGEAI
                     NVQANVHSIGGFSAHADQHGLLEWLSGFSTKPRNLFIVHGENSAQQELAGLVKQKFEW
                     EPIIPRLGERFNLETNALLEQALSQSAAAGYALDRKITDLEHLVLALHRQINMTSFER
                     NDALTGHDTTGSQIEEIRKNLTELEKLINKSA"
     gene            6173..6541
                     /locus_tag="VF531_10515"
     CDS             6173..6541
                     /locus_tag="VF531_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714442.1"
                     /translation="MKSGLGRHFSADLYLCQNTPWNTPEHFKRAIDRITPVRNDSTFS
                     WQTVKVGPDLYRFSGTLDDMLVLLQIFPDKSYLALDIISWQPQLDLQSFAESLIDLFA
                     PQVIASESRLRGEHLLDQPN"
     gene            6839..8320
                     /locus_tag="VF531_10520"
     CDS             6839..8320
                     /locus_tag="VF531_10520"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7714443.1"
                     /translation="MRVDGFDEIIRKCRELSTGHDVYYLVRIQSRKDLSVHINNGRTE
                     EISTANLEGIGLQIINSQGFMGFAASDQVNAAILEDLFAKALFLAEQSQSVAGESNRE
                     IFKLEPLCKRMEIPLRYPYTSLGLNQIEAKLMDLNREVGTYDSRLAIRTLLRLTDEEW
                     RIGRSDGTDVSFNTPRSFVFHSITAKEGSETATTYANLPGTDLEVIIEPEKLTRLKKR
                     AKKAATLATNLLTAGKIPGGHYKMVIDYALAKGLAHEAFRHAAETDNLESSILGEQGR
                     FKTGMIVADSRLSIVDGPVEGDYAYQPISAVGVERRTVTIVDHGRLQNGLADIFSAAG
                     AGVPPTGAERVESVFQLPIARMTNIRITMDDPLPLKQEFETVTPADLYRILQENKLIQ
                     PGETVLYLSGFHGGQVNPAFGDFVFHCSGIYTLGPEPVLYKPAIFSGKILSVLKSVSA
                     AIGEPRIDAMGTCGKMGQSVPSCGGAHEFLLIESNSDVMIGGE"
     gene            8322..9629
                     /locus_tag="VF531_10525"
     CDS             8322..9629
                     /locus_tag="VF531_10525"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7714444.1"
                     /translation="MELALKLEDLLHTSAPRSDLKAWRFDLHDTAGIEVGLKNNRIGG
                     PYSAPSYKKSISGELFLIWEKNRYTSAKLDAQVIAGFEDYFKLWQSTAFYDADGVGLF
                     TPAQLPEVPLVDQPAREIASGISATPFQLLDEGYRQLTGRYQAYKVDGKVRCYQDRRV
                     IINSAGLHLDYLQTPVEFYFEMNDCYGESYQEKRWPEDDKIRRIIDNTGQIGMKLGTQ
                     TTAKLTGPIRLLLPSAVFEMFLNYFLIENLFGSLVMNRQSRYTLEDFTGQRMVLREDL
                     SLTLNTLLPYRAFSYPCTAEAVPGGEMTLIDCGRLGTPFLNLKYANKAGMKPTPIPSG
                     GRGFFLKTRQPLPSWDQLVAGTESGLIVYSVLGLHTQDFSTGSFSLTADQCLLVQNGE
                     IVGKVKAVINGDFLGSLLSPESKLGSFPDEDNPGYSFIATAAT"
     gene            9787..10716
                     /gene="fba"
                     /locus_tag="VF531_10530"
     CDS             9787..10716
                     /gene="fba"
                     /locus_tag="VF531_10530"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HEX7714445.1"
                     /translation="MPLVTSKEMFKKAYAGGYAIGAFNVNNMEIIQGITEAAKEESAP
                     LILQVSAGARKYAKHVYLMKLIEAAIEDTGLPICVHLDHGEDFEICKSCIDGGFTSVM
                     IDGSKHPFEENIKVTKQVVDYAHSKGVVVEGELGKLAGIEDAVNVSDKDAAFTDPDQV
                     EEFVKRTGVDSLAIAIGTSHGAYKFKGEARLRFDILEEVQKRLPGFPIVLHGASSVIP
                     QFVEDINKYGGQMPGAKGVPEDMLRKAASMAVCKINIDSDLRLAMTASIRKHYAEKPA
                     DFDPRQYLGPAREAIKELVRHKLVSVLGCSGKA"
BASE COUNT         2868 a         2541 c         2820 g         2857 t
ORIGIN      
        1 atctgccata tctttttgcc tcttccgacc aggcttacca ggcctgggat ggtgaacccg
       61 gtcaagcaat actaaaacag tttcaaaatc cggggcttat cggggtttgc tactgggata
      121 atggtttacg ttctcttact acacaaaacc ggccggttga aaaacccgaa gatctggcgg
      181 gcctccagat tcgggtcatg gagaatcctg tttatcttga gttttttaat ttcttacatg
      241 ccaaacccac ccctttaccc tgggaagaag ttataccagc ttttgagcag ggaattatcg
      301 aggctgaaga gaattcggtt ccgataattt actggaatca cttacagaag tatcaacgtt
      361 atctgatcct gacccaacat tgttatagtc catttattgt attgaccggc ccttcgctga
      421 gacgaaaatt atccggctcc gattatgaat gggtactggc gctttttcgt aatgaacgtt
      481 tcaaacaacg ccaattgatt caggaacaga cgacccgtta tcttaaaatt ttgcaggatg
      541 gcgggatgag aatcctgact ccggatgtta agcgcttccg ggaactgggc cgggaattct
      601 cccgtaacca attgaatcac tttagccccc aattcgtcga ctatttcaag gggtatttgc
      661 aatgagaacg ccgcgaattt cgcttaaact tttccgcatc ctgggtatcc tagtgctgat
      721 actcatcgtc gctcatgccc tgctttacca gcagatgacg cggtataata tgacaaatca
      781 gctttttctc cgtggtacgg agttggcata tccgctatgg caaaaggtca atcaagtcct
      841 ggagcttggc gggaattcag aatctttata cggcttgagt tactcatgta gtcagattaa
      901 gaaaacctcg ccggatcttc gtgaagtggc ggtcgtggat gaacagggcc ggatcctggc
      961 acatgaccaa atcaaattgg tcggaaccac cgtcgcttca actccgcgta gttccggtga
     1021 gaagatgatt tttcgggaaa cccggcgctc cctcgacacc tatctgcctg tctatcactc
     1081 cgatcgcctg ccgatcttcg taaagatatc cctgtcgaaa cgccttatgt accaacagat
     1141 ccgtcgcgct ttaatgacct ccttaatctt tggtgctatc gccatcagcc ttttcatggt
     1201 tacgttgttc ttcttgacca attccttatt ggggatgcga atcacccggt tactcgaagg
     1261 ttttacagat ctttcaaaag gctgtcttac ggtacgttta acgaaccccg gcccaaccgg
     1321 gagtaaaccg ctcagaggcc gggatgaact ggatttattg atggaatcct ttgataccat
     1381 ggccaaccgc ttggagaaga gtgattttaa gcgccgcaag caggaacagc aattaagcta
     1441 tctggcaacc catgacccgt taaccggtct tcccaaccgt cgcttattgg aatacgagat
     1501 gaagaaggtc atcagccaac atcaaccggc taaatggccg gcatttctgt taatggatct
     1561 ggataatttt aaatttgtca acgataccat gggacatgcg gccggggatc aagtgctgat
     1621 aaaactaacc gctatgataa aagacatgac tctgggcaag gacctcttgt cccgctttgg
     1681 aggggatgaa ttcgcactcc tcctgacaga ttgcaccgat cctggggagg cacaatgtgt
     1741 ggcggaaaga atctgccaag cagtggctga gtaccgcttt gtttttgaaa aaaacagctt
     1801 tcatctaggg ttgagcatcg gaattgtcat gatcgacggt atgaatacgg atccgggagt
     1861 gatcctatcc caggccgata ccgctatgta ttctgccaag aggcagggac gcaatcgggt
     1921 ccaggtttac caacccgacg ataccatgat aaggctctcc gagacgagta attgggccag
     1981 ccaaattaaa gatgctctag aagaagaccg tttgcagcta ttttatcaac ctgtggtaca
     2041 cctaaccgat tcccggatcg atcattatga agcgctcgtc cggttacgta acccgaatgg
     2101 ggatctcatc gcacctcagg tttttatccc tgcagccgag caattcggcc taatgccgca
     2161 actggaccgg tgggttgtca aacatgtcat tcgagcctta caggaaaatc cggagatccg
     2221 tatcttcatg aatctctcca gtttcggcct ggcggatcag tcttttttag caaccattga
     2281 aaacgctctg gtggatgggg ggattgaacc gaagcgcctg gggttcgaga tcacggagac
     2341 cgctttggta caggatcttc acagggcgca acgttggatc gagagtctga aaattttggg
     2401 ttgccgattc gcgttggatg atttcggcgc cggtttcaac tcatttcttt atttacgtca
     2461 cctccccata gaccaactga agttggatgg gttattcatt aaaaatttgg gcagggaacc
     2521 gattcaaaaa cctttggttc aagccatgca tcaattggcc ggagctttag gcatcgagac
     2581 cgtggccgaa tgcgtcgagg atgccgagtc gctcgaaata ctgaaggaaa tcggagtctc
     2641 cttcggtcaa ggtttttatc ttggggtgcc ggtatcggtt tttgatttga atccgcttcc
     2701 taaagcgtaa aataagtagt cgctaaagtt taaaatgagg tgttcaaaat gcgggtctat
     2761 gacctgattt ggaaaaagcg tgaaggcggg gaattatcct cggtcgaact cggctttttg
     2821 gtcgacggat ttgtaaaagg agagattccc gattatcaga tgagtgcctg gtgcatggcg
     2881 gtttattttc ggggcatgac ctttgaggaa tgcaccgctt taactctggc catggcaaat
     2941 tctggagaaa ccgtggatct ttcatcactg cccgggatta aagtcgacaa acacagtacc
     3001 ggaggggtgg gggataaaac ttccctggta ttactgccac tcgtcgcggc ggccgggatt
     3061 ccggtagcta aaatgtcggg ccggggtttg ggccataccg gcgggaccat cgataaactg
     3121 gagtcaatcc ccggttttag aaccagcctt acgcaagaag aatttatgga acaggttcgt
     3181 cgttgcggaa ttgccttgat agggcagacg ggtaacctgg tgcctgcaga caagaagcta
     3241 tatgcattgc gggatcttac cgccacagtg gagagtattc cgctcatcgc cagcagtatc
     3301 gtatcgaaaa aacttgcagc cggtgccgat gcagttgttt tagatgtcaa attcggcgat
     3361 ggcgctttta tgcatgatta cgaccaggcc ttacggcttg ctcaaaccat ggtagagatc
     3421 ggtaaccggg cgggccgccg ttttcgggcg attttgagca atatggatca accgttgggt
     3481 tgggctatcg gcaatagtct tgaagtggcc gaggctattc aaactctgaa aggggaggga
     3541 ccgtctgatt taactgagct ttgtatcgtt ctcgggggga caatggttca gatggccggc
     3601 ttggcagcca ctaaagaagc gggggaacgg ctggttcggg agttgctcca ttccggtaaa
     3661 ggattggcca aattagcgga attgattaag gcccagggag gaaatccggc tgtattggat
     3721 gatcccgctc tattacccca tgctgccgtg atggaatccg tcaaggctcc tgccgacggt
     3781 tgggtggcag ggataacctg tcgtgaacta gggctggcca caatgcgact cggtgccggc
     3841 cgcaccaagc ttggagaggc aatccgtccc gaggcgggat tgaaggtttt tgcgaaagtc
     3901 ggggattttg tcaaggcagg tacagtaatc gccgaggttc acgcggtaac tcccggtgag
     3961 gcagcgagcg gtaaatccga tgtcctgcgg tgcttcacat tttcgcaaaa ggaaataccg
     4021 catccggagt tgatcagggg aaccgtcgga aacggggaga aagaggagta ggttgtcttc
     4081 gcccatttat cactcataac cttggtcctt tgcatccatt taactacttt gaatccgcct
     4141 tttcccctct ttccagtttg cgggagagag gggaaaaagg ggtgagtcga aaaggggaat
     4201 tctaataaat tcaggatttt tggcaataaa aaaaagcctt acggcttttt actcatttag
     4261 atggtcgggg tgagaggatt tgaacctccg gcctttcggt cccgaaccga acgctctacc
     4321 aagctgagcc acaccccgaa aagttacagt ataaattata acaaaacgca gctagtttgt
     4381 caaaagaaaa tttgaatctt ttcgatccat atatgccata attcccaagt caaggaggaa
     4441 taaagaatga ggttaacttt ttgcggcgcc gccggaacgg taaccggctc ctgctatttg
     4501 gttgaaaccg aacaagtccg ttttttagtg gattgcggaa tgtttcaagg taccaaggaa
     4561 attcgtgatc tcaaccagca tgccttttta ttcaatccgg gagcacttga ttttatactt
     4621 ttaacgcatg ctcatgtgga tcatgccgga ctaataccta aattatggaa attaggctat
     4681 aaaaaaccgg tatacgcaac caaggcaacg gttgcgctct ctcaaatcgt actgccggat
     4741 tcaggacata ttcaggagat ggaggccgaa tggcgtaacc gcaaacactc acggaccgga
     4801 gaacccatgc aggaaccgct ctatgatgcg gatgacgcgg ttcagtcttt aaaactattt
     4861 cggccgcaaa attataacca ggaatttgag cctgctccgg gcatcaaggt ccgtttccgg
     4921 gacgccggtc atattttggg gtctgccctg attgagctgt acatcactga gtccgggtcc
     4981 acaacgaaat tggtattctc cggggatctc ggccaaaaag gtcagccgat catcgctgat
     5041 cctacgatta ttcagagcgc cgattatatc gtcatcgaat cgacttatgg taaccgtttc
     5101 catgaagatc aggctcaaaa ggtagaactg ttaaaaaatt taatcaagga gagcattcac
     5161 agcggtggaa acctggtaat tccggcgttc gccatcggcc gcacccaaga tctcctttat
     5221 catctacggg aactatttaa ccagggggct ttaccccgta tcccggtata tatcgatagt
     5281 ccgatggcaa tttcggtcac cgagatctac cgtgacaacc ctgattgcta cgacgaagat
     5341 acattgagat tatttgccat gcatcagagt ccgtttgaat ttcccaacct ctcttttata
     5401 cggacggcag aggaatccaa gcgattgaac gagaccgcaa aaggcgcaat tatcattgct
     5461 gcaagtggta tgtgcgaggc aggcaggatt ttacatcatc ttaaacataa cctgtggcga
     5521 cctgaagcgc atatcctgtt cgtaggttat caggccgaag gtactctggg acggagactt
     5581 ttagaaggtg ccaaagtcgt gaaaattatg ggtgaggcca ttaatgtcca ggccaatgtg
     5641 cattcgatcg gtggattttc agcccacgcc gatcagcatg gattgctcga gtggctttcc
     5701 gggttcagca ctaaaccccg caatctcttc attgttcatg gtgaaaactc ggcccaacag
     5761 gaattggccg gattggtgaa acagaaattc gaatgggagc cgatcattcc acgattgggg
     5821 gagcggttca atctggaaac caacgccctt ttggagcagg ccctcagtca atccgcggct
     5881 gccggttacg ccctggaccg caagattacc gacttggaac acttggtgtt ggcgctgcac
     5941 cgtcagatta atatgacctc ctttgagcgg aatgatgctc tgaccggcca cgatacaacc
     6001 ggcagccaga ttgaggagat ccggaaaaac ctaactgaat tggagaagct gataaataaa
     6061 tcagcttaat taaagctttt tttcgggagt tgaaacccga ttgcccggcg acacatatta
     6121 tattagcaaa ttggaccgtg ttttagtcgc tcgcaaatag ggaggtatcc gtatgaaaag
     6181 cgggttaggc agacacttca gcgccgacct ttatttatgt caaaataccc catggaatac
     6241 tcccgaacat ttcaaaaggg caatcgaccg gataacgccg gttcgtaacg atagtacgtt
     6301 cagttggcag acggtgaaag taggtcccga tttatacagg ttcagcggta cattagacga
     6361 tatgctggtc ttgctgcaga tttttccgga caaaagttat ctggcgttgg acattatctc
     6421 ctggcagccc cagcttgatc tacaatcctt tgccgagtcc ctgatcgatc ttttcgcacc
     6481 tcaggttata gcttccgaat cgcggctccg gggggagcat ttattggatc aaccgaattg
     6541 agagtttgat aaccggacgt gccgcggaga atactagtag agcgtcacgg agatcaaatg
     6601 ctttgtcaac aaaattaaac agtcgttata acgaacggtc atatttgttg aaggacattc
     6661 gtatcctttg agtacaagtg ctcaagggat acgatgtccc gacagcatct tacccttggg
     6721 ggcatgaagt agtacaagcc tttcatacgg ggatttactg ttcctaaaga acgaacactg
     6781 aacgaagatt ataaaataac gcggccgccg ggtccagccg ggatagagag gtttcgtgat
     6841 gagggtcgac ggattcgatg aaattatccg gaaatgtagg gaactcagta ccgggcacga
     6901 tgtttattat ttagtacgaa ttcaaagccg caaggatctt tccgttcata tcaataacgg
     6961 acggaccgag gagatcagta ccgcaaatct ggaaggaatc gggttgcaga tcattaattc
     7021 acaaggattc atgggttttg ccgcatctga ccaggtaaat gcggcaatac ttgaagacct
     7081 cttcgccaag gcgttattcc tggctgagca gtctcaatcc gttgccggag aatcaaaccg
     7141 ggagatcttc aagctggaac cgctttgtaa gcggatggag attccgctac gttatcctta
     7201 tacaagttta ggcctgaatc aaattgaggc taaacttatg gatttgaacc gggaggtcgg
     7261 aacctacgac tcccgtttgg caatcaggac cttattgcgc ctgaccgatg aggaatggcg
     7321 aattggacgc tccgatggta ctgatgtatc tttcaatact ccccgttcct tcgtttttca
     7381 cagtatcacc gccaaagaag gctccgaaac tgctaccacg tacgctaatc ttcccggaac
     7441 cgatttggaa gtcatcattg agccggagaa actcaccagg ctcaaaaaac gggcaaaaaa
     7501 ggccgcaacc ttggcgacca atcttctaac cgccggaaag atacccggcg gacattataa
     7561 gatggtgatt gattacgctc tggctaaggg tctggcccac gaagcgttca gacacgccgc
     7621 agagaccgac aacctggaga gctcgatcct gggagaacaa ggtcgcttca agaccggcat
     7681 gatcgttgcc gattcccggc tctccatcgt tgatgggccg gttgagggtg attacgccta
     7741 tcaacccatt agcgccgttg gagttgaacg tcgcaccgtc actattgtgg atcatggccg
     7801 actccaaaac ggcctggcgg atattttctc cgctgccggg gccggagtac ctcccacggg
     7861 ggcggaaaga gtggagagtg tttttcagct tccaattgcc cggatgacga acatccgaat
     7921 tacaatggat gacccgttac cgcttaaaca ggagtttgaa acggtaactc cggcggatct
     7981 ttaccgaatc ttacaggaaa ataagttgat tcaacccggg gagacagtct tatatttatc
     8041 gggatttcat gggggacaag tcaacccggc gttcggcgat tttgtatttc attgctcggg
     8101 aatatacact ttgggtcccg aaccggtgct atacaaaccg gctattttta gtggcaagat
     8161 actatccgtg ctaaaatcgg tgagtgccgc catcggcgaa ccccgaatcg acgccatggg
     8221 tacctgcggc aaaatgggcc aatcagtacc cagttgtggc ggagcgcatg aatttttact
     8281 gattgaatcc aattctgacg tgatgattgg cggtgaatga aatggaattg gccttgaaac
     8341 tggaggattt attacacacc tctgccccga gatcggatct taaggcttgg cggtttgatc
     8401 tccatgatac ggccggcatt gaagtgggtc tcaaaaataa tcggatcggc ggaccgtatt
     8461 cggctccaag ttataagaaa agcatcagtg gtgaactctt cctgatatgg gaaaaaaacc
     8521 ggtatacctc tgccaaactt gatgcccaag tgattgcggg gtttgaagat tatttcaaac
     8581 tttggcagtc taccgccttt tacgatgccg atggtgtggg gctgtttact ccggctcaat
     8641 tgcccgaagt tcccttagtg gatcaaccgg cccgtgagat tgcaagcggt atctctgcca
     8701 caccttttca actgctcgat gaaggttacc gccagttaac cgggagatat caggcgtata
     8761 aagtagatgg caaggtccgt tgttatcaag atcggcgggt catcatcaac tcggctggtt
     8821 tgcatctgga ttaccttcaa accccggtgg aattttattt tgagatgaac gattgctatg
     8881 gtgaatccta tcaagaaaaa agatggcctg aagatgacaa gatccggaga ataatcgaca
     8941 acaccggcca gatcggtatg aaactcggaa cccaaacaac ggcaaaatta accggaccta
     9001 ttcgactcct tttaccatca gcggttttcg agatgttttt gaactacttc ctcattgaaa
     9061 acctctttgg cagtctggtt atgaatcgtc agagcagata tacattggaa gatttcaccg
     9121 ggcagcggat ggtactccgg gaggatttga gcttgacctt gaataccctc ctcccttacc
     9181 gagctttctc atacccatgt accgccgagg ctgtgccggg cggcgagatg actttgatcg
     9241 attgcgggcg actaggtact ccgttcttga acctaaaata cgctaacaaa gctggcatga
     9301 agccaacccc cataccctcg ggcggcagag gcttttttct caaaacccgt caaccgcttc
     9361 cctcttggga ccagttggtg gccggaaccg aatccggatt aatcgtctat tctgtcttgg
     9421 gtctccatac gcaagacttt agtaccggta gtttttcgct taccgctgac cagtgccttt
     9481 tggtgcaaaa cggggagatc gttgggaagg ttaaagctgt catcaacggt gattttttag
     9541 gatcattgct cagtccagaa agcaaactgg gatctttccc ggatgaagat aatccgggtt
     9601 actcgtttat tgccacagct gcaacctgaa aatgaggtgt attgctaaat tttgaatttg
     9661 gcaaaaatcc ttgattttga aattggatct ggtaaaatta ttatgtttag gaaagcggaa
     9721 tacgtttcat agattacata attttgtttt gtggaattca aatttcttaa ataaggagtg
     9781 gaactgatgc cgttagtaac gtccaaggaa atgttcaaaa aggcgtatgc cggtggttac
     9841 gccatcgggg ctttcaacgt caacaacatg gagattattc agggaatcac cgaagccgcc
     9901 aaggaagaat cggcaccttt gattctgcaa gtttctgccg gagcacggaa gtatgccaag
     9961 catgtttacc tgatgaaact gatcgaggcc gcgattgaag acaccggact tccgatctgt
    10021 gtccatctgg atcatggtga ggattttgaa atttgtaagt cttgcatcga cggtggtttt
    10081 acctccgtga tgattgatgg ttccaagcat ccttttgagg agaatattaa ggttaccaaa
    10141 caagttgtgg attatgccca ttccaaaggt gttgtggtcg aaggagaact gggtaaactc
    10201 gccggtattg aggatgctgt caatgtctcc gataaagatg cggcatttac cgatcctgat
    10261 caggtagagg agtttgtcaa acgaaccggt gttgattctt tggcgattgc cattggaacc
    10321 agccatggag catacaagtt caaaggcgag gcgcgcttac gtttcgatat tttggaggaa
    10381 gtgcaaaaaa gattgcccgg tttccccatt gtgttgcacg gcgcatcctc ggtcattccc
    10441 caatttgttg aggatatcaa caaatacggt ggacaaatgc ccggagcaaa aggagttccc
    10501 gaagacatgc tccgtaaggc ggcgagcatg gcggtttgca aaataaatat cgattccgac
    10561 ttgagactgg caatgaccgc cagtattcgt aagcattacg cggagaaacc ggccgacttc
    10621 gatccccggc aatatctggg gcctgcccgt gaagccataa aagagttggt ccgtcataaa
    10681 ctcgtaagtg ttcttggctg tagcggcaag gcttgattaa aatcaaccag tggaaacatt
    10741 tggtatttga aggtctgaga aggccttcat tttttttgat ccaacagaat tgatgagttc
    10801 gagacggtat aaaatgtcca atctgttcaa taagagttaa aaaccggtaa aaagcttgac
    10861 aatcctggtc aactcattta ttctgttggt attaaactta tttaacccga cttataggct
    10921 gaattctatc aggtaaatat ttattaccgg atggtgggcc gatgcttcgg aaccaccagc
    10981 tttttacggg atccatgatc tgaaaatacc gtcttttttt atgtcatgga ggtttctgcc
    11041 gtgatcaccc gtgtaaaccc gtgatttccg gggaaacctg gatagg
//
LOCUS       DASVMB010000368         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_191675,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000368 DASVMB010000000
VERSION     DASVMB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191675"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..3386
                     /locus_tag="VF531_10535"
     CDS             363..3386
                     /locus_tag="VF531_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714446.1"
                     /translation="MPTIGFSKLLLIGAGPTVIGRSNGLDQATAQTCRILKAEGYQVI
                     VVDSNPATLTTDRDLADGTYLEPLTASFLTKIIAKERPDALVVSCGGPMALNLGLKLA
                     CSGVLAQYGTRLLGITEAALKIMADDEEWAGTVRGAGLKFPEGVTVTDTAKSAAYLFE
                     HEFPLFVRAASHLDPAPRFLAYNREEFTELIEKELALTDNGQVRLEKSLMGWKQLEFE
                     FLIDCSGESRLIGTVEQLDPVGVHSGDSAAVLPIQSVSYEAYREILDRCRLLLKTLGL
                     VGCANLKFAVNQAADRWVLLKLTPGYNRTAQWVAGSTGLAVARIAAQLTVGHTLNEVL
                     PVRDRGPIDQGHLLVRLPRFDFRKFYDTEQTLDTVMKSVGEVVAAAPEFKAALQKAVR
                     SLAIGRSGLGSDGRDPDPAVLPLSSIRAKLINPNPDRLFFIRYALKQGLTQDEIRNLT
                     GIDPWFLKQLAELNGFEKELTTYALYNLTPQVFLRAKQWGFSDLQIAFLLRSTEDQVR
                     NARKKLGIMPDFVSVGDPENYYYHSTYDSVHTGPPPQGERPDRTQPERKRVMLVGGGP
                     NRIDHGTEHDYALYHGALAIQNSGYQGIIVNSNPAALTTLDGGHCLLFMEPVTGEDIR
                     NIVERERPDIALLQFGGKPAMELGPTLRQAGVEVLDGFEDLRISARLAELGLRDAAGE
                     SQAENLMQDAVGVEIHCITDGIGVKVIGVLEQIEEAWIDSGDSAQALPSYTLGQETLD
                     KLYHYTAHLLPGTGFKGLATVKYAIRHDQIILREVLPGISQTLPFICKTTGNDWIETA
                     IKVLCGSGLPDPGSVNALNLRYSAVKEAVFSFDHFPDIDTLLGPKKRSTGMVSGIGRD
                     FGLAFIKAELAAGDKIPTAGSIYLNLRDEDKRAFIPLIKQFMTFGFKIFSTPEIAAVF
                     SRNNLPCQAVAKVGEGRPNILDKIKSGEIQWLIDTGHRDHRDERIVRSTAVARGIPII
                     TTIAAAEAVVLGLERYLKDDFELETLQQYYSGENI"
     gene            3457..>4044
                     /locus_tag="VF531_10540"
     CDS             3457..>4044
                     /locus_tag="VF531_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX7714447.1"
                     /translation="MRKVLLILLIWALLIPTAFGAENEPMRVAVLSGNVGLSMVRLMD
                     RPEAAEYPVRFEVYNNPNPVLAKLISGDLDLAGLPTNMAAILYNKGVPVQLASVIGWG
                     VMYVVSSDATIKTWSDLRSHEIYVASKGAISDILFRYLVSKNGLDPEKDMKIQYIASA
                     AELAQLAASGKATTAAVPEPWVTEALEKDPGLKIVL"
BASE COUNT          975 a          925 c         1137 g         1007 t
ORIGIN      
        1 acaaggaaat tccctggagt ttcttttaag aaggttaaca gtactcttcc aagaacagtg
       61 gtgagatcta atttaattgc tgtaacatgg aaggggattt attgaaatca gtagaataat
      121 tgtttatcga ggcagtttga cccaaggcga aggtatattt ggtaacaacc aaggataatt
      181 ctataatggt acccaaaact gcgggctctc ttttgccgaa acatctccct ccatacgggc
      241 caaggatgta tgcttcaagc gaatgttgca tttttgcctc ttgagtcggg atgtttcggg
      301 aggagtatgg cccatccgaa gtggcgtgta cgtggcagaa ccgatataaa gggatgattg
      361 taatgcctac tataggtttt agcaaattat tgctgattgg cgccggccca accgttatcg
      421 gacggagtaa cggattggat caggcgacgg cacaaacttg ccggatttta aaagccgaag
      481 gttaccaggt cattgtggtt gacagtaatc cagctacatt gacaacggat cgggatttgg
      541 ccgatggaac ttatctggag cctttgactg catcgttttt gacaaagatc atcgccaaag
      601 aaaggccgga tgctctggtt gtgagttgcg gcggtccgat ggctctgaac ttgggcttga
      661 aattagcctg ttccggggta ctggctcagt atggtacgag gttgctggga attaccgaag
      721 cagcgctaaa gataatggcg gatgacgagg aatgggccgg gacggttcgg ggtgcggggc
      781 tcaagttccc ggagggggtt accgtaactg atacggcaaa aagtgctgcg tatctctttg
      841 aacacgaatt tcccctgttc gtcagggcag cttcccattt ggatcccgct ccccgctttc
      901 tagcttacaa ccgggaagaa ttcaccgaac tgatcgagaa ggaactggct ttaaccgata
      961 atggccaagt taggctagag aaatcgttaa tgggttggaa acagcttgaa ttcgaatttc
     1021 tcatcgattg cagcggagaa agccggttga tcggaaccgt ggagcagctc gatccggtgg
     1081 gggttcattc cggggacagc gcggccgtgc ttcccatcca atcggtttct tacgaagctt
     1141 atcgtgaaat actggatcgt tgccgtttgc tgttaaaaac cctgggactg gtaggttgcg
     1201 ccaacttaaa atttgccgta aatcaggccg cagaccgttg ggtattgctc aagctgaccc
     1261 ccggatataa tcggaccgcc caatgggttg caggatccac cggtttggcg gtggcgcgga
     1321 tcgccgctca actgacggtc ggacatacgc tcaacgaagt gttgcccgtt cgggacagag
     1381 ggccaatcga tcagggacat ttattggttc ggttaccgcg atttgatttc cgtaagtttt
     1441 atgataccga acagaccctg gatacggtga tgaagtctgt gggtgaagtt gtcgccgccg
     1501 ctccggagtt taaagccgcc ctgcagaaag ccgtgcgttc cctggctatt ggccgttccg
     1561 gcctgggttc cgacggccgc gatccggacc ctgccgtttt acccttaagt tcaattcggg
     1621 caaaactaat caatcccaat ccggaccggc tttttttcat tcgctacgcg cttaagcagg
     1681 gattaaccca ggatgagatc cgcaacctca ccgggatcga tccttggttt ctaaagcagc
     1741 tggcggagtt gaacggcttc gagaaggaac tcaccactta cgcgttgtat aatcttacgc
     1801 cgcaagtgtt tctaagggca aaacaatggg gtttctccga tcttcagatt gcattcctcc
     1861 ttcgatccac cgaggatcaa gtccgcaatg cccgtaaaaa gcttggaatc atgcctgact
     1921 ttgtgagtgt gggagatccg gagaattatt attatcattc tacatatgat tcagttcata
     1981 cgggtccgcc tccccagggt gagcgaccgg acaggaccca acccgaacgg aaaagagtca
     2041 tgttggttgg cgggggtcca aaccggatcg atcacggtac ggagcatgat tatgccctat
     2101 atcatggggc tctggctata cagaattcgg ggtatcaggg gatcatcgtc aacagtaatc
     2161 cggcggcgct tacgactttg gacggaggcc attgcctgct ttttatggaa ccggttaccg
     2221 gggaagatat tcgcaatatt gtggaacgag agcggccgga tattgcgttg cttcaattcg
     2281 ggggcaaacc cgccatggaa ctcggcccga cattacgcca ggcgggagtg gaggtcctgg
     2341 atggattcga agacctgcga atctcagccc gactggcgga gcttggattg cgtgatgcgg
     2401 cgggggagtc gcaagcagag aacctcatgc aggatgcggt cggcgttgaa attcattgta
     2461 tcaccgatgg gataggcgtc aaggtgattg gagtgttgga acagattgag gaggcttgga
     2521 tagactcggg agattctgct caggctctgc cttcgtatac attgggccaa gaaacgctgg
     2581 ataaattgta tcattatacc gcccatctgt tgccgggaac ggggttcaaa ggattggcca
     2641 cagtcaaata cgccatacgc cacgaccaga tcattctcag ggaagtgctt ccgggcatca
     2701 gccaaacgct cccgttcatc tgcaagacta cggggaacga ctggatcgag actgccataa
     2761 aagtactatg tggatcgggc ttgccggatc cgggaagtgt aaatgctctt aatctacgtt
     2821 atagcgcggt caaggaagct gtatttagtt tcgaccattt tccggatatc gataccctgc
     2881 ttggaccgaa gaaacgatcc accgggatgg tttcaggtat cggtcgtgat tttggtctgg
     2941 cgttcatcaa agcggagttg gccgcgggcg ataagatacc gaccgccgga agtatctatc
     3001 tgaatcttcg ggatgaggat aaacgggctt ttataccctt aatcaaacaa tttatgacat
     3061 ttggattcaa aattttttca acccccgaga tcgccgcggt cttttcgagg aataacctac
     3121 catgtcaagc tgttgccaag gtgggggaag gaagaccgaa tattctggat aagattaaga
     3181 gcggcgagat ccagtggttg atcgataccg gccaccggga ccaccgtgat gagcggattg
     3241 ttcgtagcac cgcggtggcc cgggggattc ccatcatcac cacgattgca gcggcggaag
     3301 cggtggtctt gggactcgaa cgatatttga aggatgattt tgagcttgag acgcttcaac
     3361 agtattactc cggagaaaac atttaaattt ctcattatca atttcaaagg ccaagtaagt
     3421 tagctaatcc gatttgcatg agaggagttt tcataaatga ggaaggtact tttgatctta
     3481 ttgatttggg ccttgttaat cccgactgct tttggcgcgg aaaatgaacc gatgcgagtg
     3541 gcggttctct ccggtaatgt aggcctttcg atggtgaggt taatggaccg gcccgaggct
     3601 gccgaatatc cggtccgctt tgaggtatac aacaatccca atccggtttt ggccaaactg
     3661 atcagcgggg atctggatct ggccgggctt cccaccaata tggcagccat tctttataac
     3721 aagggcgtcc cggttcaatt ggcctcagtc atcggttggg gtgtcatgta tgtggtcagc
     3781 agcgatgcca ccattaaaac gtggtcggac cttagaagcc atgaaatata tgtggcatcc
     3841 aagggcgcga tctccgatat tctctttcgg tatctggtga gtaagaacgg gcttgatccg
     3901 gagaaggata tgaaaattca gtatattgca agtgctgcgg aacttgccca acttgcggct
     3961 tccggcaagg ccacaaccgc tgcggttccc gaaccttggg tcaccgaggc gctggagaaa
     4021 gaccccgggc tcaagattgt tttg
//
LOCUS       DASVMB010000369         6207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_191798,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000369 DASVMB010000000
VERSION     DASVMB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191798"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VF531_10545"
     CDS             complement(<1..1038)
                     /locus_tag="VF531_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7714448.1"
                     /translation="MQAEIIDPLEKIKLEPRGLPELSQVETELYRELEGGPGPIRELT
                     SMIHAGGKRIRPQLVLYSGLLFSGSVTTGMVRTAVAAELIHTASLIHDDIIDGASLRR
                     HQPSFNQTRGVQIAVLAGDYLLARSLGILAGEGLSTVMAGLTGTICEMCRGEFFQAEE
                     RFNFGLTLEAYYQRIASKTAILFQFCCTGGATAGGAAMNQLATLREFGLHLGLAFQIR
                     DDLMDWCGDPEQLGKPGHTDMSQGTITLPLLFLFQDRAFAEKFQKRLVHEGGNATKII
                     SPDLVREIEGWLWSSGAIARSEGVITAHLIRAKRVLQQLPFGRARDGLSGLIRELESE
                     WAQDRYCNPDPP"
     gene            1294..2028
                     /locus_tag="VF531_10550"
     CDS             1294..2028
                     /locus_tag="VF531_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7714449.1"
                     /translation="MKKYSWIAILLVAVMVSGLALATKPVLTVGTDATYAPFESIDKN
                     GNFVGFDMDLMRLVADQTGMELKLVNINWDGIIPGLMNGNYDCLISAMTITPERKKQI
                     NFSDPYFSIRQAIVVKKDNNTIKGPKDLEGKTVTVQNGTTGDLYASKIKSIKMKRFDT
                     NPQAIQELLNNNADAAVMDDLVAISAIKTASGLKMVTIEDIEPENYGIGIRKDNTALL
                     AKINQAIDALKKNGKLAALEKQYLGK"
     gene            2111..2365
                     /locus_tag="VF531_10555"
                     /pseudo
     CDS             2111..2365
                     /locus_tag="VF531_10555"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
     gene            2396..3082
                     /locus_tag="VF531_10560"
     CDS             2396..3082
                     /locus_tag="VF531_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7714450.1"
                     /translation="MAETFIYYMWLNLPSLLLGALMTLLLTCVAVFDGIVIGTLAGVA
                     RVVHNKVLNLIAGIYVDFIRGTPLLVQIFMIHFGSPPLLGALFNHGNPIPINPFVSAL
                     VALSINSGAYVAEIVRSGIQSIDRGQMEAARSLGMTYSQAMRFIVLPQALKRVIPPLG
                     NEFIAMLKDSSLVSVIGMEELVRKAQIIVTRSYRPTETWLEVGVLFLIMTIPFTRIVA
                     KLEKRLKVSD"
     gene            3075..3800
                     /locus_tag="VF531_10565"
     CDS             3075..3800
                     /locus_tag="VF531_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7714451.1"
                     /translation="MIRVENLQKRFRHLEVLKDISTHVKQGEVVCIIGPSGSGKSTFL
                     RCLNLLEKPSGGHIYIEGVDVSDHSLVDVNEVRAEVGMVFQRFNLFPHKTALENVTIA
                     PVKVRGLSPEEARERAYELLDRVGLRQKADTYPEHLSGGQQQRIAIARALAMHPKALL
                     FDEPTSALDPEMIQEVLDVMKQLARDGMTMVVVTHEMGFAREVAHRVLFLDEGAILEE
                     GTPEDIFSHAQNDRTRQFLSKIL"
     gene            3815..4720
                     /locus_tag="VF531_10570"
     CDS             3815..4720
                     /locus_tag="VF531_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7714452.1"
                     /translation="MKLKLNAADLLLIVTTMIWGWSFIIVKWSISLIDPFFFIFIRFL
                     LALAVLTLCFFPKIKGHWRTCLKPGIFLGIILTLGFITQTLGLTYTTASASGFITGLN
                     VILVAVFASMINRRLPRRIVLVGIICATVGLLLITLKGRLSFAQGDLLTLACALFFAI
                     HVVYTDRLAQNLSAAILTLVQFATVTVLSGTAYLCFGRQAATLGQFSVLQWLALFYAG
                     VPATALAYLFQTKAQQKIPAFRTAVLLATEPMFAGIFALSLGFDPFSWKVVAGGLLIV
                     FGMICASWDTRNGSGEIPQGSQVSL"
     gene            4958..6148
                     /locus_tag="VF531_10575"
     CDS             4958..6148
                     /locus_tag="VF531_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7714453.1"
                     /translation="MKHHFLKESLILSLICLFIFGSISYTQSLNKQSDSLALVHCSII
                     DGTGAPPLKDRIILINKGRIKQIGAAGDIAIPAEYQKIDLAGAFVLPGFINAHVHNGY
                     NETNLQRWLQAGVTTVRDLGPHGSFNFVKERDRLNLNPLNTRLVSATPLITKPQGYGS
                     CYINSPEEAERQVTVFIKSGIDVIKIAIEDNLQGRVWPMLTSAEVRRIVNSAHTLKKR
                     VSAHISHTRNLQIAIDAGVDDISHMVVEPLDEKQLHQIVEKNIYWEPTLELWQGVSKI
                     HSLDWDRITLANLARFYKAGGKVALGTDFDGYVCQFDRDFPITEVALMQQAGMSNMDI
                     IIAGTRNAAYVCDLERELGTIEQGKIADLLVVGRNPLDDLKVLQKPYLVIHNGLVVNK
                     GNSN"
BASE COUNT         1560 a         1616 c         1465 g         1566 t
ORIGIN      
        1 gggggggtcc ggattgcaat accggtcttg agcccattcc gactcaagct ctcggattaa
       61 cccggataac ccgtccctcg cccggccgaa cggtaattgc tgcaataccc gtttggcccg
      121 tatgagatgc gcagtgatta ccccctcgct tcgggcaatt gcgccgctac tccagagcca
      181 cccctcgatc tcccgaacca aatccgggga tataatctta gtggcgtttc cgccctcatg
      241 cacgagccgt ttttggaatt tctctgcaaa agcccggtcc tggaacaaaa agagtaaagg
      301 cagggtaatg gtaccctggg acatatcggt atgccccggt tttcccaatt gctcggggtc
      361 gccgcaccaa tccataagat catcccggat ctgaaaagca agtcccagat gtaacccgaa
      421 ttcccgaagt gtcgcaagct gattcatggc ggctccaccg gcggttgctc caccggtgca
      481 gcaaaattgg aacaaaatgg ctgtttttga agcaatcctt tggtaatagg cctccaaagt
      541 caacccgaaa ttaaatcgct cctcggcctg aaaaaactct ccccggcaca tctcgcagat
      601 tgttccggta agtccagcca ttacagtgct taaaccctct ccggctaaga tacccaagga
      661 ccgggccagt agataatcgc ccgccagtac cgcaatttga acaccccgcg tctggttgaa
      721 ggatggttgg tggcggcgga gtgatgctcc atcaatgatg tcgtcgtgaa tcagtgacgc
      781 cgtatgtatc aactctgccg caaccgctgt ccttaccatg ccggtagtga ccgacccgct
      841 aaagagtaac ccgctgtata agactaattg aggcctgatc cgttttcctc cggcatggat
      901 cattgaggtc agttcacgga tgggaccggg tcccccctct aactcgcggt acaattcggt
      961 ttctacctga ctcagctccg gcaagcctcg cggttccaac ttgattttct ctaaaggatc
     1021 gattatttcc gcttgcaact gttcccctcc cccaaaacca aaagacctca acttatgtga
     1081 aaacgtccag gtctgatacg agtagtatga ccgcatctgc tgctttcaac acctgcattc
     1141 cgccgactga aaaatgaata aatatactaa tacgaataac tgtttcccaa ttaattctag
     1201 accttttttt gttacagttt gacaaactct ttgcacggtt gccggcgatg ttatataatg
     1261 atcttaaaca ttcatccgaa aggagtttca aaaatgaaaa aatattcatg gattgcaatt
     1321 ctactcgtgg cagtaatggt atcgggtctc gctttagcca ccaaaccggt tttaaccgtc
     1381 ggtaccgacg ctacatacgc accgtttgag tcgatcgaca aaaacggaaa ctttgtcggt
     1441 ttcgacatgg atctgatgcg cttggttgcg gaccagacgg gaatggagct caaactggtt
     1501 aacatcaact gggacggaat cattcccggg ctgatgaacg gaaactacga ctgcctgatc
     1561 tcggccatga ccatcacccc cgaacggaaa aaacaaatca atttctccga tccttatttc
     1621 tcgatccgcc aggcgattgt ggtcaaaaag gataacaaca ccattaaagg gcccaaagat
     1681 ctggagggta agaccgttac ggtccagaac ggcaccaccg gcgaccttta tgccagtaaa
     1741 atcaaaagta ttaagatgaa acggttcgat acgaaccccc aggcgattca ggaattattg
     1801 aataataacg ctgatgcggc tgtaatggat gatctggtag cgatctccgc gatcaaaacc
     1861 gcctccggtt taaagatggt tacgatcgag gatatcgagc ccgagaatta cgggattgga
     1921 atccggaagg acaacaccgc cctgctcgcg aagatcaacc aagccatcga cgccctaaaa
     1981 aagaatggta aattggcagc actggaaaaa caatacctcg gcaaataatt gaattggctt
     2041 aagtatggtc aagaggcctt ccggcttctt ggccatacat ggtttttaaa gccaccaaag
     2101 ggtgtttagc gtgaaaacaa aagtttacat cggtctggct ttacttttag ttttgtttct
     2161 ggccatatcc ggtagtgcct tcggagccgt tcgcaacgat tcccgtttaa aaataagctc
     2221 cgatccggcc ggcgcccagg tttggatcga cggccgaaac accggacgat tttctccctg
     2281 cgtcatcctg ggtttgagca ttggcgatca ccgggtggaa cttcgaaaac ccaagtaccg
     2341 taacgagatg caaactgttg caatccaacc cggagataac agcgccgact ttgcaatggc
     2401 ggaaacattc atctattaca tgtggttaaa tcttccctca ttactgctcg gtgcgttaat
     2461 gaccctcctt ttgacgtgcg tggcggtatt tgacgggatc gtcatcggta ccttggccgg
     2521 agtggcccgg gtagtgcata ataaagtctt gaatttgatc gccggaatct atgtggattt
     2581 tattcgcgga actccgctct tggtccagat cttcatgatt catttcggat caccgccgct
     2641 tttgggtgcg ctctttaatc acggcaatcc gatccccatc aatccctttg tctcggcatt
     2701 agtcgcgctc agtatcaaca gcggcgccta cgtggccgag atcgtccgct ccgggattca
     2761 gtcgatcgat cgcggtcaga tggaggccgc ccgttccttg ggaatgacat acagccaggc
     2821 gatgcggttc attgtactgc cccaagcctt gaaacgggtc atcccgcccc tggggaacga
     2881 gttcattgcg atgcttaagg attcctcatt ggtctcggtg atcggcatgg aggaactggt
     2941 ccgcaaggcc cagatcatcg taacccgttc ctaccggccg accgaaacct ggcttgaggt
     3001 aggcgtcctc ttcttgatca tgacaattcc tttcacccgg atcgtggcca agttggaaaa
     3061 gaggttgaag gtcagtgatt agagttgaaa atcttcaaaa acgttttcgc cacctggaag
     3121 tattgaaaga catatccacc catgtaaaac agggtgaagt tgtatgtatc attggcccca
     3181 gcggatccgg aaagagtact ttcctccgct gtctcaattt acttgaaaaa ccatccggtg
     3241 gtcatatcta tattgaaggc gtggacgttt cggatcattc actggtggat gtgaacgagg
     3301 tccgggcgga agtcggcatg gtttttcagc ggtttaacct ttttccacac aaaaccgctc
     3361 ttgagaatgt cactatcgct ccggtcaagg taagggggtt atccccagag gaagctcgtg
     3421 aacgtgctta cgaattattg gaccgggtcg gactacgtca aaaggccgat acctaccccg
     3481 aacacctttc cggtggccag cagcaacgga ttgccattgc ccgggctctg gcgatgcacc
     3541 ccaaggcgtt actctttgac gaacccacat cggcgttgga tccggaaatg atccaggagg
     3601 ttctggacgt catgaagcag ctggcgcggg atggaatgac catggtcgta gtcacccatg
     3661 agatgggttt tgcacgagaa gtggcacacc gggttttatt tctcgatgag ggcgcgatct
     3721 tagaagaagg tacccccgag gatatcttca gccatgctca aaacgatcgg acccggcagt
     3781 tcttgagcaa aattctataa aacaaggtgg cacaatgaaa cttaagctga atgccgcaga
     3841 tttgctatta atcgtcacta ccatgatttg gggttggtcc ttcatcatcg tcaaatggtc
     3901 gatctctctg atcgaccctt ttttctttat attcatccgc tttttactgg ctctggccgt
     3961 tttgacgctc tgcttctttc ccaaaatcaa gggccactgg cgaacctgcc tcaagccagg
     4021 catcttctta ggaatcatcc tgacgctcgg tttcatcacc caaactctgg gactaacgta
     4081 cactaccgct tcggcctcgg gtttcatcac cggtctgaac gtcattttag tagcggtctt
     4141 cgcatccatg atcaaccggc ggctaccacg acggatcgtc ctggttggta tcatctgcgc
     4201 aaccgttgga ttactcttga ttactcttaa agggcggctg agtttcgccc aaggtgatct
     4261 gcttaccctg gcctgtgccc tcttcttcgc catccatgtg gtctataccg atcgcctggc
     4321 acagaatctc tcagccgcca ttttgaccct cgtccagttt gctaccgtca ctgtgctatc
     4381 gggaacggct tacctttgtt ttggccgaca agccgctact cttggacagt tctccgttct
     4441 gcagtggctg gccttatttt acgccggagt gcccgccacc gccctggcat acctgttcca
     4501 gaccaaagcc caacagaaga ttcccgcttt ccggaccgcg gtgcttctcg ccaccgagcc
     4561 gatgttcgcc gggatctttg ctctgagtct cgggttcgac cccttcagtt ggaaagtcgt
     4621 agccggcgga ttattaatcg tcttcggcat gatctgcgcc agctgggata caagaaacgg
     4681 gtcgggagag atcccgcaag gcagccaagt atctctctaa atcttttatt ttccttccgc
     4741 tcttcaacaa ggaagtacaa ccacccattc tatgccaaac tcgatttatt acaagctcaa
     4801 agtatccttc atctaagaaa aaaatactaa ttagggtgag ttgcttactt ttttcaggaa
     4861 accctaatta cgatgagtct ccaaatcaag ttaccgcaca ataggaaacg gagttttgtg
     4921 aggaatccat caaacttaaa ccggtatcgg aggagctatg aaacaccact tcctgaaaga
     4981 gagtttgatc cttagtttaa tttgtctatt catctttggc agtataagtt acacccaatc
     5041 cctgaataaa cagtcggatt ccctggcgct cgttcattgc tcaattattg acggaaccgg
     5101 tgctccgcca ctcaaagaca ggataatttt aataaacaaa ggccggatca aacagatcgg
     5161 agctgccgga gatattgcca ttccagcgga atatcaaaaa atagacctgg ccggagcatt
     5221 tgtattgccc ggttttataa atgcccacgt ccataacggc tataacgaaa ccaatttgca
     5281 gcgctggctt caagccgggg tgaccaccgt acgggatctg ggaccccatg gcagttttaa
     5341 ctttgtaaaa gaacgggatc gattgaattt aaatccttta aacacccggc tcgtatcggc
     5401 tacaccgttg attaccaaac cccagggata tggcagttgc tatataaact cccccgaaga
     5461 agccgaacgt caagtgacgg tgtttattaa gagcggtatc gatgttatta aaattgctat
     5521 tgaagacaat ttacagggcc gggtctggcc aatgttgact tctgccgagg ttcgaagaat
     5581 agttaattca gcccatactc taaaaaaacg agtttcagcc catatttcgc acacccgtaa
     5641 tctccaaatc gcgatagatg ccggagttga cgatatctcc catatggtag tcgaacccct
     5701 cgatgagaag caactccacc agatcgtcga gaagaatatc tactgggaac ccaccctcga
     5761 actctggcaa ggagtcagca agatccactc tctggattgg gaccggatca cccttgccaa
     5821 tcttgcccgt ttttacaaag ccggaggtaa agttgccttg ggtacggatt ttgacgggta
     5881 cgtctgccaa tttgatcgtg actttcccat caccgaagtg gccctcatgc agcaagccgg
     5941 catgtccaat atggatatta tcatcgcagg aacccggaat gctgcctatg tttgtgattt
     6001 ggaacgagaa ttgggaacga ttgagcaggg gaaaattgcg gatttactgg tcgtcggcag
     6061 aaatcccctt gacgatctga aggttttgca aaaaccatac ctggtcatcc ataatgggct
     6121 ggttgtcaac aagggaaata gcaattgaaa cggtatccgg ttaccccgtt tccagtcaaa
     6181 aataatcaag ataaaatgtt tccttgt
//
LOCUS       DASVMB010000370         5704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_46782,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000370 DASVMB010000000
VERSION     DASVMB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46782"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..885
                     /locus_tag="VF531_10580"
     CDS             79..885
                     /locus_tag="VF531_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7714454.1"
                     /translation="MKITSISNDLVKMASGLHHKKGRLEQNLYLAEGIHLVQEAYKSG
                     AHIRKIFWSERLLATDEGRELLRTLQERFEEVAVGDAVFEKIAETENPQGIVATIELP
                     KDQAPDWNRIHLGLIIDGLQDPGNLGTIIRTAWAAGLDGLFLTSETADPYQGKAVRAS
                     MGGIFRQSIYRNFEPEWLIHHARQAGIQIIAADPQAGMELFQCDLQIPTLFIVGNEGR
                     GLNPVWEDFSLKRVKIPQPGKAESLNVAVATGILIYEALRQRLVMASCKK"
     gene            965..1066
                     /locus_tag="VF531_10585"
     CDS             965..1066
                     /locus_tag="VF531_10585"
                     /inference="COORDINATES: protein motif:HMM:NF025372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HEX7714455.1"
                     /translation="MTLSADFFWKVFETTGSITAYLIYREIIRTTMV"
     gene            1439..2458
                     /gene="pheS"
                     /locus_tag="VF531_10590"
     CDS             1439..2458
                     /gene="pheS"
                     /locus_tag="VF531_10590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7714456.1"
                     /translation="MKEQLEQLRAAALAEIERAQDSEALNEIRVKILGKKGSLTAILR
                     GMGGLPAAERPVIGELVNQIRQEIEAKLETFRQRFAAREQEAKLIAEELDIQLPGRKP
                     RVGHNHPLQLVREEIEEIFLGLGFSIAEGPEVETDYYNFEALNLPKDHPARDMQDSLY
                     FTQEVLLRTHTSPVQIRTMERLYPNPIRIIAPGRVYRRDAIDASHSPMFIQLEGLVVD
                     QGITFGDLKGVLTVFAKKLFGADRQIRLRPSFFPFTEPSTEVDVSCGCGGKGCRVCKG
                     TGWLEILGAGSVNPRVLSMSKYDPDEVSGFAFGMGLDRIAMLKYGINDIRTFYENDQR
                     FLAQF"
     gene            2529..4937
                     /gene="pheT"
                     /locus_tag="VF531_10595"
     CDS             2529..4937
                     /gene="pheT"
                     /locus_tag="VF531_10595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7714457.1"
                     /translation="MRVSFEWLQEYVKINIPVAELAERLTRSGIAVEAIEDQAAAFQG
                     IVTTKVEKLEKHPQADHLWITRLDAGKQGIRTVITAAQNLKLHDVVPVALPGTTLLSG
                     KVIGTAVLKGITSEGMMCSGEELGLEKESAGIWVFAENVPIGVPVAEIFGATDFVLEL
                     ELTANRSDCMGIIGVAREVAAILGAEVHLPDTTVREEDPAMESLIRAEIAAPDLCPRY
                     SGRVITDVKIEPSPEWMQRRLRAAGVRPINNLVDITNYVMLECNQPLHAFDLDKIHER
                     QLIIRSAEPGEQLVTLDDAVRKFSRGELLIADPQGGLCVAGVMGGANSEVTDKTKNIF
                     LESAWFNPISIRKTARALNMKTEASLRFEHGIDPNGTVFAANRAAHLIGELHAGKITR
                     GILDLNPRPEKPATIQTSAERINRWLGTDLGASQIQNYLERLNFQVTGSGSGQLTATA
                     PSYRRDIFHMADLAEEVARLYGYDQITATIPESRQLGERTPFQQLQRDCRHLLTGLGL
                     TEVITYSLYAKNTPARLGLSPEDPLARTTELLDPLSEDQAVMRTNLVHSLLETVEFNT
                     KRRQSNLFLYELARVYWPKTDTVLLDEPLHLGAVLTGRPHETGWNQGLDEVDFYDLKG
                     IMERLFQEFHFPAFTMEQAKRPFLHPGQAAEVYVNGRSVGFLGQIHPAVLERYELNQK
                     VFLMELDLSYLAPLAQTEVHFEPLPKFPAVERDLALVVNRTLPASAIEAKIRELGGEM
                     VERVELFDVYQGNQVPDGMRSLAFGLTYRAKDRTLSDNEVNQLQEGLLRKLAEIYQAK
                     VREA"
     gene            5111..>5704
                     /locus_tag="VF531_10600"
     CDS             5111..>5704
                     /locus_tag="VF531_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714458.1"
                     /translation="MKTLHGWWSAPRGNRDIWMIPAGYLVFLLIWFLFSIELYGHEGP
                     RSYFELSAVFVSKFWLVALVFLCRQLLFPESGWPVCFRRLLILFSLGFFGLELTKMME
                     YKAAWLYGLCVLGDGLCWSVALASFFDNLGMIYRFWILWQPSLTDYEVIRLRFQSDLL
                     KLGLVWMAGLGLIYSYLVNFYLVDSVLYSRITAGIDLL"
BASE COUNT         1382 a         1337 c         1593 g         1392 t
ORIGIN      
        1 ccacccgccg gttagcgaca gattagcgcg gatcgaagct ttaccataag tgacgcatta
       61 gggttcggag gatggaacgt gaagattacc agtatttcca atgatttggt gaaaatggca
      121 agtggtttgc accataaaaa aggccgttta gagcagaatt tatatttggc ggaaggaatt
      181 catctggtgc aggaggccta caagtcaggt gcgcatatcc gcaaaatttt ttggtcggaa
      241 cggctgctcg cgaccgacga aggtcgggag ttgctaagga ctcttcaaga acgcttcgaa
      301 gaggttgccg ttggcgacgc ggtcttcgaa aaaatagcgg aaaccgaaaa tccccagggg
      361 atcgtggcaa caattgaact tcccaaagac caagccccgg actggaaccg aattcacttg
      421 ggactcatca ttgatggcct tcaggatccg ggaaacctcg gcacgataat ccgtaccgct
      481 tgggctgccg gtctcgacgg cttgttttta acgtcggaaa ccgccgatcc ctatcaggga
      541 aaagcggttc gcgcttcgat gggggggatc ttccggcaat caatctatcg taattttgag
      601 cctgaatggc tgattcatca tgcgaggcag gcggggatcc agatcatcgc agccgatccc
      661 caagccggga tggagctttt tcaatgtgat ttgcagatcc cgacgttatt cattgtaggt
      721 aacgaaggtc ggggtttaaa cccagtttgg gaagattttt ctttaaaaag ggtaaaaatt
      781 ccacagccgg gaaaggccga atcattgaat gttgcagtag cgacaggcat tttgatttat
      841 gaggccctca gacaacgctt agtgatggcc tcttgtaaaa aataaagtgc tctgttataa
      901 taatgctaag cattgcggtg accgttttca cgacaatagt cagtgaaaaa ggggatgatt
      961 acagatgaca ctatctgctg attttttttg gaaagttttc gagacaacag gttctatcac
     1021 cgcctacttg atttacaggg aaattatccg aaccacaatg gtttaggctg aaatacttag
     1081 ggctcttcca tttggacaca attgaatatg tctaaagtaa tgatggagga aagtacttga
     1141 ctttgggaag gttttaggga gaaaggccgt gactgaaagc ctttcccgga ccggtcgagg
     1201 aaggttccct ccggaactgc gagctgaaaa cgggctttca cgccgccagt aggtgtagac
     1261 ggcactccac cgttaaccgg agagggtatc ggagtaaccg gtttggaaac ttcccgtacc
     1321 ttgaatgagt ttgcacattt atgcgcaaaa ttgttgggtg gtaccacgag attaaccttt
     1381 cgtcccatga gggatgaagg gtttttttat tttaccgatt tttagggagg ttcatgggat
     1441 gaaagaacaa ttagagcagc tacgtgctgc ggccttggca gagatcgaaa gggcacaaga
     1501 cagtgaggct ctcaacgaaa ttcgcgtgaa aattttgggc aaaaaaggct cactcactgc
     1561 gattctaagg ggaatgggcg ggctacccgc cgcggagcgg ccggtcattg gcgaattagt
     1621 caatcagatt cgccaggaga tcgaggccaa actggaaacg ttccgccaga gatttgccgc
     1681 gcgggaacaa gaagccaaac tgattgcgga agaactggac atccagttgc cgggacgcaa
     1741 acctcgcgta gggcataacc atcccctgca attggtcagg gaggagattg aggagatctt
     1801 tttggggctc gggttcagta tcgccgaggg tccggaagtg gagacggatt attacaactt
     1861 cgaggcgcta aatttgccta aggatcatcc ggcccgggac atgcaagact cgctctactt
     1921 tacccaggag gttctactcc ggacccatac ctcgccggtg cagatccgga ccatggaacg
     1981 gctttacccc aacccaatcc ggatcatcgc acccggacgg gtttaccggc gtgatgccat
     2041 tgatgccagc cattcgccga tgtttattca actggaaggg ttggtagtgg atcaaggcat
     2101 tactttcgga gatctcaaag gcgtacttac cgtcttcgcc aaaaaactct tcggggctga
     2161 tcgccaaatc cgcctgcggc cgagcttttt cccgtttacg gaacccagca ccgaggtgga
     2221 tgtctcttgc ggctgtggcg gtaagggttg ccgggtgtgc aagggtaccg gatggcttga
     2281 gattttgggt gccggctcgg taaaccccag agtcctgtcg atgtccaaat acgatccgga
     2341 cgaagtatcg ggatttgcct ttggaatggg actcgaccgg atcgcgatgt taaaatacgg
     2401 gataaatgac atccggactt tttacgagaa tgaccagcgc tttttagctc agttttaagc
     2461 cactacgttc cgggagcagt cggtccggaa ttgagtatta ttaaagtttc ggatttggag
     2521 gagatagaat gcgcgtatcg tttgaatggt tacaggaata tgtaaagatt aatataccag
     2581 tggcggagct ggctgaacgg ctgacccgct ccggaattgc cgttgaggcc attgaggatc
     2641 aggcggcggc gttccagggg attgtcacaa ccaaggttga aaaacttgag aaacatcccc
     2701 aggccgatca tctctggata acccggcttg atgcgggaaa acagggaata aggaccgtaa
     2761 tcaccgcggc acagaactta aaactccatg atgtggtgcc cgtggcttta cccgggacca
     2821 cactcctaag cggcaaggta atcggaaccg ctgttttaaa gggtattacc tctgaaggaa
     2881 tgatgtgctc cggtgaagag ttggggctgg agaaagaatc cgcggggatc tgggtttttg
     2941 cggagaatgt ccccatcggc gtgcctgtag ccgagatttt cggcgccacc gatttcgttc
     3001 tggagctgga attgaccgcc aatcgttccg attgtatggg aataatcggt gtggcgcgtg
     3061 aggttgcagc gatcctggga gccgaggttc atcttccgga tacaacggta agggaggaag
     3121 acccggccat ggaatcattg attcgggcgg agattgccgc tccggatctc tgccctcgct
     3181 acagcgggcg ggtcatcacc gatgtgaaga ttgagccgtc tccggaatgg atgcaacgac
     3241 gcctcagggc agccggtgtc cggcccatca ataacctggt ggatattacc aactatgtaa
     3301 tgctggaatg taatcagccg cttcatgcct ttgatcttga caaaattcac gaacggcagt
     3361 tgatcattcg tagcgccgaa ccgggagaac aactggtaac cttggatgat gcggttcgca
     3421 aattttcccg gggtgagcta ttgatcgccg atccccaagg cggtttatgc gtcgccggag
     3481 tgatgggcgg ggccaatagc gaagttaccg ataaaaccaa aaacatcttt ttggaatcgg
     3541 cctggtttaa tccgattagt atccggaaaa ccgcccgggc gttgaatatg aagaccgagg
     3601 cgtcgctccg tttcgaacat gggattgatc caaacggaac cgtctttgct gccaaccgtg
     3661 cagctcacct tatcggggag ttacatgccg gcaaaattac ccgcgggata ttggatttga
     3721 acccccggcc cgagaaaccg gccacgatcc aaacttcggc tgaacgaatc aaccgttggt
     3781 tgggcaccga tttgggcgct tcccaaatcc aaaactattt agaacgcctt aatttccaag
     3841 tgaccggttc cggttcgggc cagcttacag cgactgctcc ttcctaccgg agggatatct
     3901 tccacatggc cgatctggcc gaagaggtag cccgattgta cggttatgac caaatcaccg
     3961 cgaccattcc ggagagcagg cagttggggg aacggactcc attccagcag ttacagcggg
     4021 attgccgcca tcttctgacc gggcttggcc tcaccgaagt catcacatac agcctgtatg
     4081 ccaagaatac gccggcgcgt ttgggcctta gccccgagga tccgttggcc cggaccacgg
     4141 aattactgga tccgctcagc gaggaccagg cggtgatgcg gaccaatctg gttcatagcc
     4201 ttttggaaac agtggagttc aataccaagc ggcgtcagtc caacctcttc ctttatgagc
     4261 tggccagggt ttactggccg aagactgata cggtcttact cgatgaaccg ctccatctgg
     4321 gagcagtctt aacgggaaga ccccacgaaa ccggttggaa tcaaggtttg gatgaggttg
     4381 acttttacga tctcaagggg ataatggaaa ggctctttca agaatttcat tttcccgctt
     4441 tcaccatgga acaggcaaaa aggccttttc tccatcccgg gcaggcagcc gaggtatacg
     4501 tgaatggccg ttcggtggga tttttaggcc agattcatcc ggcggtttta gagcggtatg
     4561 aactgaatca aaaggttttc ctgatggaac tcgacctttc ttatctggcc ccccttgcgc
     4621 aaacagaggt tcatttcgaa ccactcccga agtttcccgc agtcgagcgt gacctggccc
     4681 tggtggtcaa ccggacgctc ccggcatccg ccattgaggc aaagattcgg gaactcggag
     4741 gggaaatggt tgagcgagtc gaactcttcg atgtctatca gggtaaccag gtacccgacg
     4801 gtatgcgcag tctggcattt ggacttacgt accgggctaa ggatcggacg ctcagcgata
     4861 atgaagtcaa ccagcttcag gaaggactct tacggaaact ggccgagatc tatcaggcca
     4921 aggtcaggga ggcctaaaaa aggcaacagg gaacaggcac tagggcgaaa ggacaattta
     4981 ttgctgtatt tagggtccct gttgccgcgc gccccaaggg gcgcgaccct attccctgtt
     5041 gcctaaagaa ggttctaaac ctcctccggc ggtacatagg attgtaccaa aaaactggag
     5101 gtaaaaaccg atgaagacgc ttcatgggtg gtggtcggct cctcggggga accgcgacat
     5161 ttggatgatt cccgccggtt atttagtatt tttgctgata tggtttttgt tctcaattga
     5221 attatacggg catgagggtc cgaggagtta ctttgagtta agcgcggttt ttgtaagtaa
     5281 attttggttg gtggcattgg tcttcctctg ccgccaactt ttatttcccg aatccggatg
     5341 gccagtgtgc tttcggagat tactcattct tttttcgctt ggattttttg gtctggaatt
     5401 gaccaaaatg atggagtata aggcggcctg gctatacggg ttatgtgttt tgggagacgg
     5461 actctgttgg agtgtcgctc tggcttcatt ctttgataat ctaggaatga tctaccgttt
     5521 ttggatcctt tggcaacctt ccctgaccga ttatgaagtc attcggctcc ggtttcaaag
     5581 tgatcttctc aaactcggac tggtatggat ggccgggtta ggtctcattt actcttatct
     5641 ggtcaacttc tacctggttg attcggtcct atatagtcgg ataacggcag gaattgatct
     5701 gctc
//
LOCUS       DASVMB010000371         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_341286,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000371 DASVMB010000000
VERSION     DASVMB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341286"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VF531_10605"
     CDS             <1..866
                     /locus_tag="VF531_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714459.1"
                     /translation="LLIALILNQKIRGIKFFRAAYYMPSITSSVVITIIFMWLFSRQG
                     VVNFLLTRCKHNVLVLLTFLVLFFLFQTGFYLYEKWRRRPVAYFEGSFLLLSLILSVA
                     ITFILKTGGVIPTFGDTAPVDISWYNTRDVWPNWAGKLAFPIPLGTIMIQNVWTTAPT
                     FMLLYLAGLQDIPGYLLEAAAVDGATKWKSIRYIVIPQLKHVTYLVVTMGLIGTLQMF
                     DQAAVVGDQAPLDSIITIAYYAYNNAFNASGISKTGLACASAIILSLFILIIVLIQRG
                     LLKVGGGIDEE"
     gene            856..2265
                     /locus_tag="VF531_10610"
     CDS             856..2265
                     /locus_tag="VF531_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714460.1"
                     /translation="MKNKATKPAGERFLKPREFLLDDVRRARWAKVGFIYTLLLLISI
                     IFMGPLLFGALSSLKDQPLEWPPRIETNQLKFHNWMGVYRSAKKGGGHGFFGEFTPGT
                     EVPFEVTYRVQSGMEPVPPTVQVPRRVAGTTVLVNRSEHYAADYTEIGPVQEITRKKL
                     ADGSLWVRYGFMIKHTGNMVLQQLPLDLTVPFGEVYAAATFDPNRIERQGRVQSWNNL
                     TSGLIPYVFHNYHRIFNENYSRSSGKSMFVSWFLNSFNYSIIRVLTTILFASMAGYAL
                     ARLRFKGKNFIFVTMLFSMMVPSQVTFISNYLVLRDGIFGLTRLFGIQTLLNSYAGLI
                     ISGVVGASSVFIMKQFFQGMPGDMEESAKLDGASTYQAFFRIYLPLAKPAMSALTILT
                     FQGVWNEFFWPLVVINAPQERFPLTVGMLSLRRTYGVYYDWGPILAASIVAILPIIVV
                     FIVFQRYFIEGINFSGSKG"
     gene            2296..>3791
                     /locus_tag="VF531_10615"
     CDS             2296..>3791
                     /locus_tag="VF531_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714461.1"
                     /translation="MAKYLDAIDDDTVNSPGGLGDQSLGQDDPLFFRIREGNTENLFY
                     RNGPVAAHILCTSGEIPKMIVAFPAGNSGLGIWFKGSGHPVDLRVCDPMREIQSREGL
                     FGVRFAVEASAPELILEEGVLGSIRVIRDFATNHHLDDALKWVVKVTGQGVRAFRDRL
                     DGKCRYETEIKPGRGTVIIEKDGLLVFQAPQNTGNHPIRFTLTVLCGERPLTPIPAGE
                     LLKEEAGTDERLQKVLTFLSYDEKLAAGSWRFLTYFGRDTELSLVMLMPALKSRGIEA
                     GLSSLIERLNDYGEVAHEEEIGEFAVIRHAGDRTCDKQDTPIYDYKMIDDDLLLAPLL
                     ARFFIDCPQAGKSVGRFLDKTTICGRSYRDAVFRNLSLVLSRARSFAQSPSITTLLAL
                     KANETVGNWRDSANGLGGGKIPYDVNVALMPVALQATAGLFARGLFGPKQDLADQAID
                     LAKAWQEAGDYFKISVPKEKACQLVREYAITVGVPAEDAVASIDRDIN"
BASE COUNT          886 a          829 c         1026 g         1050 t
ORIGIN      
        1 cattactgat agcgctgatt cttaaccaaa aaattcgcgg gatcaagttt tttcgggccg
       61 cttattatat gcccagtatc acatcgagcg tagtcattac aatcatcttt atgtggcttt
      121 ttagccggca gggtgtggta aatttcctgc tgacccgttg taaacataat gttttagtct
      181 tattgacctt tttggtcttg tttttcctct ttcagaccgg cttttatctt tatgaaaagt
      241 ggcgcaggag gccggtggct tattttgaag gcagttttct gcttctttct ctcattctga
      301 gtgtagccat aacttttatt ttgaaaaccg gcggggtgat cccgactttt ggtgatactg
      361 ccccggttga tataagttgg tacaataccc gggatgtatg gcccaattgg gccgggaaat
      421 tggcttttcc aatacccctt gggacgatta tgatccaaaa cgtctggacg actgctccaa
      481 catttatgct actatacctg gccggtttgc aggatattcc cggatattta ttggaggcag
      541 ccgcagtgga cggggctaca aaatggaaga gcatcagata catcgtcatt cctcagttga
      601 agcatgtaac ctatttggta gtcacgatgg gattaatcgg aaccctgcaa atgttcgatc
      661 aggcggctgt ggttggtgat caggcgccgc ttgactcgat cattactatt gcctattacg
      721 cttataataa cgcgttcaac gcttcgggaa tctccaagac aggactggct tgcgcttccg
      781 caatcatcct ttcactcttt atcctgatca ttgtcctgat tcaaagagga cttcttaaag
      841 taggaggcgg gatcgatgaa gaataaggct accaagcccg caggggagag attccttaag
      901 cctagggaat tcttgctcga tgacgttcga cgcgccagat gggcaaaagt tggatttatt
      961 tatactttac tgttgttgat ttcgataata tttatgggcc ccttattatt cggcgcttta
     1021 agcagcctta aggatcaacc gctcgaatgg ccgccccgga ttgaaaccaa ccaactcaag
     1081 tttcataatt ggatgggggt atatcgttcg gccaagaaag gcgggggcca cggttttttt
     1141 ggagagttta ccccggggac cgaggttcct tttgaggtga cgtatcgggt gcaatcgggg
     1201 atggaaccgg taccgccaac ggttcaggtc ccccgcaggg tggcgggaac gacggttctg
     1261 gtcaacagat cggagcatta cgcggcagac tatacggaga tcggtccggt gcaggagatt
     1321 accagaaaga aattggcgga cggttcgctg tgggtacggt acggttttat gattaaacat
     1381 acgggtaata tggtcctgca acagttaccg ctggatctga cggtcccctt cggggaggtc
     1441 tatgccgctg caacatttga tccaaaccgg attgaacggc agggccgggt gcaaagctgg
     1501 aataatttga cctcgggact cattccctat gtctttcata actatcaccg catttttaat
     1561 gaaaattaca gccgatcctc aggcaagagt atgttcgtca gctggttttt gaactcattt
     1621 aattacagca tcattcgggt gctgacgacc atactttttg cttccatggc cggttacgcc
     1681 ttggcacggc tgagatttaa agggaaaaat tttatttttg tcacgatgtt gttttcgatg
     1741 atggtcccgt cacaggtgac atttatctcc aattatttgg tcctaaggga cgggatattc
     1801 ggtttgacca gactattcgg tattcagact ttacttaaca gttatgcggg tttgatcatc
     1861 tccggtgtgg tcggagccag ctctgttttc attatgaaac aattcttcca ggggatgccc
     1921 ggtgatatgg aagaatcggc caaactggac ggggccagta cgtatcaggc ttttttccgc
     1981 atttatttgc ccttggcaaa accggcgatg agtgccttga cgatcctgac atttcaggga
     2041 gtttggaacg agtttttctg gcctttggtc gtgatcaacg cgccgcagga aagatttcct
     2101 ttaactgtcg gtatgttgag cttgcggcgg acttatgggg tctattatga ttgggggccg
     2161 attctggctg cttcgattgt cgcgatactc ccgatcattg tggtgtttat tgttttccaa
     2221 agatatttca ttgaaggaat caatttctcg ggaagcaagg gttgatcatg atacacacac
     2281 atggggtgga tggatttggc caaatatctg gatgcaattg acgatgatac cgtgaattcc
     2341 ccggggggac tgggcgatca atcccttggg caggatgatc cgctcttttt tcggatccgc
     2401 gagggaaata ccgagaatct tttttaccgg aacgggccgg tagcggcgca cattctctgt
     2461 acttcggggg aaattcccaa gatgattgtg gctttccccg cgggcaacag cggcttgggc
     2521 atctggttca aaggaagtgg ccatccggtt gatctgcgcg tctgcgaccc gatgagggag
     2581 attcaatccc gggaaggcct ctttggggtg cggtttgcag tggaagcctc tgctccggaa
     2641 ttaattctcg aagaaggggt tttaggaagc attcgtgtga ttcgggattt tgcgaccaat
     2701 catcatctcg atgacgcact caaatgggtg gtcaaggtaa cgggtcaagg agtcagggct
     2761 ttccgggaca gactggacgg aaaatgccgt tacgaaacgg aaatcaaacc cgggcgcggg
     2821 acagtcataa ttgagaaaga cggtttgttg gtttttcaag cgcctcagaa cacaggtaat
     2881 catccgatcc gctttacact cacggtttta tgcggggaaa ggccgcttac gccgattccg
     2941 gcaggggaat tgcttaaaga ggaggccgga acggatgaaa ggctccaaaa agtccttacc
     3001 tttttgagct atgatgaaaa gttggccgcc ggatcctggc gtttccttac ttatttcgga
     3061 agagataccg agttgtcttt ggtcatgctg atgccggcgc tgaaatcacg gggcattgaa
     3121 gccggtttga gttcgctcat cgaacgattg aacgattacg gggaagttgc tcacgaggag
     3181 gagatcgggg aattcgccgt tatccgtcat gcgggggatc gaacctgcga taagcaggat
     3241 actccgattt atgattacaa gatgattgat gacgatctat tgctggcccc gttactagcc
     3301 aggttcttta tagactgccc gcaggctgga aagagtgttg gccgcttttt ggacaagacc
     3361 actatctgcg gacggtcgta ccgggatgct gtttttcgta atctttcatt ggtgttaagc
     3421 agggcccgtt cttttgcgca gtccccgtca atcaccacct tactcgcact aaaagccaac
     3481 gaaaccgttg gaaactggcg cgatagcgcc aatggtttgg gcgggggcaa gataccctat
     3541 gatgtcaatg tggccttgat gccggtcgcc cttcaagcaa cagccggatt atttgcccgt
     3601 ggattattcg gtccaaaaca agatctggca gatcaagcaa ttgatctggc caaggcatgg
     3661 caagaagcag gagattattt taagatttcg gttcccaaag aaaaagcgtg tcaacttgtg
     3721 agagaatatg caataacggt cggagttccg gccgaggatg cggttgcttc gattgacagg
     3781 gatatcaact a
//
LOCUS       DASVMB010000372         4992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_636442,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000372 DASVMB010000000
VERSION     DASVMB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636442"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /gene="mnmG"
                     /locus_tag="VF531_10620"
     CDS             <1..1659
                     /gene="mnmG"
                     /locus_tag="VF531_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7714462.1"
                     /translation="TYLNIRRINESRGPAVSALRAQADKRLYQAEMTIRLERQPNLSV
                     KQGMVTAIMVEQGRVIGVELKSGRRYFSKAVILATGTFLGGEIVMGDVKYPGGRQGEP
                     AAVELSDSLRHSGLRLRRFQTATPPRIRRQSISFDRIKPQPLQPVDWGFSWDGLAAPR
                     HQEPCWITQTTPATIQVIRENLDLSPIRSGAITTKGPHFCPSIDRKVINFPEKTDHLI
                     FLEPEGVFTEEVYLLGLTTAMPETVQERIMATIPGLENVEIIRPGYAVEYDCLDSFQF
                     TPALESKCVSNLFTAGQLNGTSGYEEAAVQGIVAGINAVRKMRGQDLFIPDRTSSYMG
                     VLIDDLTTLGTEEPYRMMTSLAEFRLGLRLDNALSRLGPYGHELGLVSGERWDGFQGL
                     LKAAVELREYLKNTKVPMTSSFWRAGDFRPERGYRLEELVLHPEITEARLYEEFPALG
                     CFPQPVREFVFNELRLAGYLERQAALLAETRRMNRVVLPEKLDYPAIPQLSPAGREEL
                     ARVEPRTLGQALRIPGLNEADRVALLLFFETAKGESENEDGAVV"
     gene            1643..3523
                     /gene="mnmG"
                     /locus_tag="VF531_10625"
     CDS             1643..3523
                     /gene="mnmG"
                     /locus_tag="VF531_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7714463.1"
                     /translation="MEPSYDLIVVGAGHAGCEAALAASKMGCRTLLFTMNYGGIALMP
                     CNPSIGGPAKGHLVREIDALGGVMGRVIDHTYLQMRLLNTQKGPAVWALRAQADKQVY
                     QRTMIRVLEDQPNLELRQGEVVDFLVDGGRIAGVKTRTGLSFGAKAVVLATGTYLEGR
                     VFIGTVNFASGPMGQLPANCLSAALGRCGIPLRRFKTGTPARVRRRSIDFSRMTPQPG
                     GFIEHGFSFWEKWEVRPDYLCWLTYTQPETHRIIREHLQEAALYTGDITGVGPRYCPS
                     IESKLIQFPDKERHQVFIEPEGADTEEMYLAGLSTSLPEYVQEIFLRTIPGLEKLEIV
                     RPAYAIEYDAIPAAELKLSLEVKKIPGLFSAGQINGSSGYEEAAAQGLIAGINAAQYV
                     KGREPLIIKRSEGYIGVLIDDLVTKESDEPYRVLTSRAEYRLTLRADTADERLTPYGI
                     ELGLINSEAREDFKAKRQRLSGLLQGLEAHTITPEPKIRQIFASAGVSVPTNRVSLGE
                     LAKRPDVELQLLQQLAPDLQLQSPEALEATIRLKYQGYLDKEREQVTRLANLENRRIP
                     ENIDYEHVTNLAREAREKLNKFKPTTLGQASRISGINPADLTALLFYLENEYRRAVEE
                     RP"
     gene            3520..4239
                     /gene="rsmG"
                     /locus_tag="VF531_10630"
     CDS             3520..4239
                     /gene="rsmG"
                     /locus_tag="VF531_10630"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7714464.1"
                     /translation="MNQTETSFNDALREAGIVLDPAVLEKLLAFIRLLLDTNQTLNLT
                     AITDYQEALYKHLYDSLIIMLRPEFQTARRILDLGSGAGIPALPLAVVAPDKTVVTLE
                     ATRKKVQFQEEACRQLGIGNCRPTWGRAEEAAQKGTERESYDLVVARAVAATNVLAEL
                     TIPFAKPGGFIALYKAKDYQAELDVSASAITTCGAAVTQCLTVELPMNYGTRALILLQ
                     KLFPTPDAYPRKPGIPQKRPL"
     gene            4751..>4992
                     /locus_tag="VF531_10635"
     CDS             4751..>4992
                     /locus_tag="VF531_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7714465.1"
                     /translation="MKRSAKNSLEILSKKAHELLSSDFEILHGYELNHQAKMFRREEG
                     KAMGKAVAIANQKGGVGKTTTAVNLGACLAQNGKKV"
BASE COUNT         1244 a         1201 c         1377 g         1170 t
ORIGIN      
        1 acctatttaa atatccgccg gatcaacgaa agccggggcc cagccgtttc ggcgcttcgg
       61 gctcaggccg acaagcggct ttatcaggcg gaaatgacaa tacgcctgga acgtcagccc
      121 aacctctccg tgaaacaggg aatggtcacc gccataatgg ttgaacaagg gcgagtcatc
      181 ggggtggaat taaaatcagg gcggcgttat ttcagcaagg cagtgatcct cgctaccggg
      241 acttttttag gcggcgagat cgtgatggga gatgtcaaat atccgggagg aagacagggg
      301 gagccggccg cggtggagct cagtgacagc ctgagacaca gcggtttacg gttacgccgc
      361 tttcaaaccg caaccccgcc acgaatccgg cgccagtcga tcagctttga tcggattaaa
      421 ccccaaccat tacaaccggt agattggggc ttttcctggg atggattggc ggcgccgcgc
      481 caccaggaac cttgctggat tacacagacg actcccgcca caattcaagt gatccgggaa
      541 aaccttgatc tttcgccaat ccgttccggg gccatcacca ctaaggggcc tcatttttgc
      601 ccttccatcg atcgaaaggt gatcaatttc ccggagaaga ccgatcattt aattttttta
      661 gaacctgaag gagtatttac cgaggaagtc tatctcctgg gcctgacaac agccatgccg
      721 gagacggttc aggaacgaat tatggcaact atccccggac ttgagaatgt ggagatcatt
      781 cgcccgggtt acgcagtgga atatgactgt ctggattctt ttcaatttac accggccctg
      841 gaatccaaat gtgtgtccaa tctctttacc gccggccagc tcaacgggac ttccggttat
      901 gaggaggctg cagttcaagg aattgtggcc gggattaacg cagtacgtaa aatgcggggt
      961 caagacctat ttatccccga tcggaccagt tcttatatgg gggtgctgat cgacgatttg
     1021 acgaccctgg gtaccgaaga accttaccgg atgatgactt ccctggcaga gttccggctt
     1081 ggcttacggc tggataatgc cttgtcccgg ctggggccat acggtcatga actgggcttg
     1141 gtttcagggg agcgatggga cgggtttcaa ggtttattaa aagccgccgt cgagctccgt
     1201 gagtatttga aaaataccaa ggtaccgatg acttcatcgt tctggcgagc gggtgacttc
     1261 cggccggaac ggggatatcg cctggaggaa ttggtgttac atccggagat taccgaggcc
     1321 cgtctttacg aagaatttcc ggccctgggc tgcttccccc agccggttcg ggagtttgtc
     1381 tttaatgagc tacgattggc cggttacctt gaaagacagg cagccctgct tgccgagacc
     1441 cgtaggatga atcgggtggt tttaccggaa aaattggatt atccggccat tccccaactc
     1501 agcccggcag gccgggaaga actggcgcgt gttgagcccc gtactttggg tcaggcattg
     1561 cggattcctg gactgaatga ggctgatcgg gtggcgttgc ttcttttttt tgaaacagct
     1621 aaaggagaaa gcgagaacga agatggagcc gtcgtatgat ctgattgtgg tcggagcggg
     1681 tcatgccggg tgtgaggcag ccttggcggc ctcgaaaatg ggctgccgta ccctgctttt
     1741 tacgatgaat tacgggggta ttgctttgat gccatgcaat ccctcgattg gaggtccggc
     1801 caagggacat ttggtccggg agatcgatgc cctgggtgga gtcatgggcc gggtgattga
     1861 ccacacctat ttacagatgc ggttgctaaa cactcaaaag ggtccggcag tctgggcgtt
     1921 gcgggcccag gcagacaaac aagtctatca gcgaaccatg attcgggtgc tggaggacca
     1981 gcccaatcta gaactccgcc aaggcgaggt cgtggatttc ctggtggatg gggggcggat
     2041 cgcaggggtc aaaacccgga ccggtttgag cttcggtgcg aaggctgtcg tcctggctac
     2101 cggcacctat cttgagggtc gggtatttat cggcactgta aattttgctt cgggtcccat
     2161 gggacagctt cctgcaaact gtctctcggc tgctttgggc cgttgtggaa ttccgttacg
     2221 ccgtttcaag acgggaaccc cggcgcgggt tcgccggcgt tccatcgatt ttagccggat
     2281 gactccccaa ccgggaggtt ttatcgagca tggtttctcc ttttgggaaa aatgggaggt
     2341 ccggccggat tacctatgtt ggctgacata tacccagcct gaaacccatc gtatcatccg
     2401 ggagcatctc caagaagcgg cgttgtatac aggggatatc actggggttg gcccgcgtta
     2461 ctgcccatcc attgagagta agctgatcca gtttcccgat aaagagagac atcaggtatt
     2521 catcgagccg gaaggggcgg ataccgagga gatgtacctg gccggtctct ccaccagttt
     2581 acccgagtat gtccaagaaa ttttcttgcg gaccatcccg ggcttagaga aattagagat
     2641 cgtccgtccg gcctatgcca ttgaatatga cgccattccc gccgcggaac tcaaactatc
     2701 cttggaagtg aagaaaattc cgggcctctt tagcgctggg cagatcaacg ggagttccgg
     2761 atacgaagag gcagctgccc agggattgat cgccggaatc aacgccgccc aatacgtcaa
     2821 gggcagggaa ccattgatca tcaaacgttc cgaaggctat atcggggtcc ttatcgatga
     2881 tttggtgacc aaggaaagtg acgagccata cagggtttta acctcccggg ctgaatatcg
     2941 tttaactctt cgggcggata ctgcagacga acggttgaca ccttatggca ttgagcttgg
     3001 tttaattaat tctgaagcca gggaggattt taaggccaag cggcaacggt tatctggttt
     3061 actacagggt cttgaggccc acaccatcac tcccgaacca aagatccggc agatctttgc
     3121 ctcggccggt gtgtctgtgc ctaccaacag agtatctctg ggagaactgg ccaaaagacc
     3181 cgatgtcgaa ctgcagttat tacagcagtt ggctccggat cttcagcttc agtcgcccga
     3241 ggcattagag gctacaatcc gcttgaaata ccaaggatat cttgataaag aaagggaaca
     3301 agtaacgagg ttagcgaact tggaaaaccg gcggattccc gagaatattg attatgagca
     3361 tgttaccaat ttggcacggg aagcccgcga aaagttgaat aaattcaaac cgaccactct
     3421 tggtcaggct tcgcgaatta gcgggattaa tccggcggat ctgaccgcgc tcctgtttta
     3481 tctggaaaac gagtatcgcc gggcggtgga ggagaggcca tgaatcagac cgagacatcc
     3541 tttaacgatg ctctgcgcga ggccgggatc gtcctggatc cggcagtact cgaaaaactt
     3601 ttggctttca tcaggctttt gttggatacg aatcaaaccc tcaatttaac ggcgatcact
     3661 gattatcagg aagcgctcta taaacatctt tacgactcgc tcatcatcat gctccgtccg
     3721 gaatttcaaa ccgcccggcg gattttagat ctgggcagcg gcgcggggat cccggctctg
     3781 cccctggctg ttgttgcccc ggacaagact gtggttactc tggaagccac ccgcaagaag
     3841 gttcagtttc aggaggaggc ctgtcgccaa ttaggaatcg gtaattgccg tcccacatgg
     3901 ggtcgggccg aggaagcggc tcaaaaggga acggaacggg agagttatga tctggtagtg
     3961 gcgcgggcgg tagctgcaac caatgttctg gcggagttaa ccattccctt cgccaaaccg
     4021 ggaggattca tcgctcttta taaggcgaaa gattatcaag ccgaacttga tgtatctgca
     4081 tcggccatca ccacttgcgg cgcagcagtt actcaatgtt tgactgtgga attacccatg
     4141 aattacggaa cccgggcttt aatcttgctt caaaagttat ttcccactcc ggacgcttac
     4201 ccccgcaaac ccggaatccc tcaaaagaga ccgttgtaaa accgaggctc gaagatttcg
     4261 agagaaatcc aaaggaaacc tgagagctca caaatagatg gaatttattc ccatttaatt
     4321 ttttgttatt tcatggattt ggtggatgga aaaccgttct cactttagtc gccccgattc
     4381 ctattccgga agaaaaccaa atagaacacg aaaagtgaag agataacact caactgcaca
     4441 atccttgatt gtgagccgta ttattttcgt gtgtttgatg tgtttcgtct gagaaggcaa
     4501 aagataacga actacgaaac acacgaaaat cacgaaaatg aaatttttca ctttcacttt
     4561 tcgtgttatt cggtaaatgt ctctcccagc ggtgcggcat tatttttcaa gcgatcaggt
     4621 tgtaccaaaa aaaccggata aatgagaaaa aattattaaa ttacagtata aaattccgga
     4681 tgaaagcagg aagtttgggg ctgatctcga aacaagaaag aagataattt cgggatagag
     4741 gaggatccgc ttgaaacgct cagccaaaaa ttcattggag atattaagta aaaaagcgca
     4801 tgagttgttg agtagcgact tcgagatcct ccatggatat gaactaaatc atcaagccaa
     4861 aatgttccgg agggaggaag gaaaggctat gggtaaagct gtcgccattg ccaaccaaaa
     4921 aggcggagtc ggaaagacga ccaccgcggt gaacttaggc gcttgtttag cgcagaacgg
     4981 caagaaggta tt
//
LOCUS       DASVMB010000373         4686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_341514,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000373 DASVMB010000000
VERSION     DASVMB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341514"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF531_10640"
     CDS             complement(<1..288)
                     /locus_tag="VF531_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714466.1"
                     /translation="MNNETLNRIIKETGSNNRTRSGNLIYQIMLYFFLIAGAVVMVFP
                     ILWMISLSLKSPAEIFSFQLNLFPKQPLWSNFVDGWVRSGFSKYFLNTFIIA"
     gene            complement(293..1171)
                     /locus_tag="VF531_10645"
     CDS             complement(293..1171)
                     /locus_tag="VF531_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714467.1"
                     /translation="MNRHFKWQVMVPYIFLLPQLLLVGAFAFYPLVYNLILSFQDVGM
                     ISRGFVGLDQYSLLLKDEIFWTSLKNTIYYTIGTVPLTTIVALLAAFALNQQIAGKKF
                     LRTVYLFPYLISWVVIGLVWQWMYSTNYGIFNHLLNLVGLPGLRWLQDPKMTIPSIII
                     ASVWHDLGYYMVIFLAGLQSISPIYYEASMIDGANKWDQFRRITLPLLKPILFIVLVL
                     SMINAFKVFDQIYVMTGGGPGRASLMLVNYILSVALTEMRLSYAAAISIVLFIIIMIL
                     TVLQRVFFKDSDTEVA"
     gene            complement(1449..2717)
                     /locus_tag="VF531_10650"
     CDS             complement(1449..2717)
                     /locus_tag="VF531_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714468.1"
                     /translation="MQIRKVTVLSVLCISLVGSLFATTLAAPKQITLSVLYDYVTEAG
                     RASVQKSLDLYQKKHPNIKLNVQVVTQAKWAEVVKTRAIANDLPDVAMAVPQRVGEWA
                     TVGKLEAGNKYIPKSVLNKYSKARLLSDTYNDGQIYGLPFADSVRAVAYNADYFAKAG
                     IKPPTKAKDAWTWDQMVEAAKKAQAASGAKYALQFGKASMDSWLAFIYQAGGALMSDD
                     FSKATLDTPEVRKALEWTVKLHKDGIAAPGIIDGTEDPLRLFASGATTLWMPTDNSLI
                     LNLSSQAKFKFGFTFMPRDAQQATVIGGSDWIAFKGNHPKESWDLVLYMVSASPMKDF
                     NEALTAIPPRSDVHVKWSVHPEFASFFAEQARQIPEALIKQQLHPAYGATRDKLLQEL
                     SACVSGQKSVDDALAAMNQYMNDSLKQNHY"
     gene            complement(3005..3679)
                     /locus_tag="VF531_10655"
     CDS             complement(3005..3679)
                     /locus_tag="VF531_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714469.1"
                     /translation="MKEIKVLLVDDQMLFVESLRTVLETRAEDIQVLGVALNGRQALE
                     LASAEEPDVVLMDVRMPEMNGVECTRMLKERFPQVKVLMLTTFDDDEYVVEALRLGAA
                     GYLLKDVPPTDLISTIRAVYQGGVMISPKVASKLVEKLVQVNQGDTDQLPLSPQSPVW
                     LKELSNREKDILRLLAKGCDNKEIAKRLYIAEQTVKNYVSIIYSKMGVKDRVQASRMA
                     AEAGLD"
     gene            complement(3684..>4686)
                     /locus_tag="VF531_10660"
     CDS             complement(3684..>4686)
                     /locus_tag="VF531_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7714470.1"
                     /translation="VRAGILVLLGFPFGSYLGIELTLFGALIIETFAYTSLRFGLVFT
                     ILVTGVLITIQRPMMAWGMILPVATPHDRVSFAMFVAMLTTLSAMIRFQRDNQISSKE
                     LNRYLNEATLQLAQANLELQDYAVMAEQEAISNERKRLAREMHDTLAYTLTNLVMMLE
                     ASLDLSSGNREELLNHLRLARDQAKEGLVGVRSALQALRPVHLEEENGLTAIYHLIQT
                     FTKATRIKVELNLGDAPLILGEEADWAAYRLVQEGMTNALRHGKATLIEISFYQERNG
                     VRINIRDNGKGMENSSQGGFGLLGMRERIEKLGGELSANNQAGKGFVLSAWIPLKEG"
BASE COUNT         1226 a         1222 c         1097 g         1141 t
ORIGIN      
        1 ggcaatgatg aatgtgttca aaaagtactt actaaatccg gaacgtaccc agccatcgac
       61 gaagttggac cagagcggtt gcttgggaaa gagatttaat tgaaaggaaa agatctccgc
      121 cggagatttt aacgataagg atatcatcca taggatcgga aagaccatta caaccgcccc
      181 ggctatcagg aaaaagtaaa gcatgatttg gtatattaaa ttccctgacc gggtacggtt
      241 gtttgatccg gtttctttga tgatccggtt gagagtttca ttattcataa gtttaggcca
      301 cctccgtgtc gctgtcttta aagaataccc gctgcaacac tgtcaggatc ataatgatga
      361 taaacaaaac aatggaaatc gccgccgcat agcttagccg catctcggtc aaggccacag
      421 acaagatgta attgaccagc atcaacgaag cccggcccgg accaccgccg gtcattacat
      481 agatctgatc aaaaacctta aaagcattga tcatcgagag taccagtacg atgaagagga
      541 tcggctttaa taaaggaagc gtaatccgtc ggaactgatc ccacttgttg gctccgtcaa
      601 tcatcgaggc ctcgtaatag attggcgata tggattggag cccggccaaa aaaatcacca
      661 tatagtaacc caggtcatgc cagacactgg caatgatgat tgaaggtatg gtcattttcg
      721 gatcttgcag ccaacgcaga ccgggaagac ccactaaatt taaaagatgg ttgaatatgc
      781 cgtaattggt tgagtacatc cattgccaga cgaggccgat aactacccag gaaatcagat
      841 acggaaagag atataccgtc ctcaaaaact tcttccctgc gatctgttga ttcagcgcaa
      901 aagccgcaag taacgcgacg atggtagtta agggcactgt accgatagta taataaatgg
      961 tgttcttcag cgaggtccaa aagatctcgt cctttaaaag cagggagtat tgatccagac
     1021 ccacgaaacc ccgggaaatc atgcccacat cctgaaaact caaaatcaag ttgtatacca
     1081 gagggtaaaa cgcgaacgcc ccaaccaaca gcaactgtgg gagcaaaaaa atgtaaggaa
     1141 ccataacctg ccatttaaaa tgacgattca tttttccttc cccttgtcgg ctcgccggat
     1201 gggtatactt tggccaaact tcctccggcg aacgtgatga tgatggatct cccatttcct
     1261 aatttaaatt tatcacagcg cctttcgaaa gtctgcctac cgtggtactg ttattaaaga
     1321 tgcaaacgca gatgattggt actcccgtac cggtaccgga catatggttt atcgccccaa
     1381 aacaaaaggc cgtccgccgg gagactttcc cgacgaacgg cctgctgatt gaggcaaaac
     1441 cattagaatc aataatggtt ttgtttgagg ctgtcgttca tatattggtt cattgcggcc
     1501 agggcgtcat ccactgattt ctgaccgctt acgcaggcac taagttcctg gagcaacttg
     1561 tcgcgggttg ccccataggc gggatgcaac tgctgtttga taagggcttc cggaatctga
     1621 cgcgcttgtt cggcaaagaa cgaagcgaac tccggatgaa cgctccactt gacatgaaca
     1681 tcgctgcgcg gcggaatcgc ggtcaaagcc tcgttgaaat ccttcatcgg actggcactt
     1741 accatataaa gcaccagatc ccaggattcc ttgggatgat ttcccttaaa cgcaatccag
     1801 tcagatccgc cgataaccgt ggcctgttgg gcatccctcg gcataaaggt gaaaccgaac
     1861 ttgaatttcg cctgggaact aaggtttaat atcaatgagt tatccgtcgg catccagagg
     1921 gtggtggcgc cggaggcgaa caagcgtaac ggatcctcgg tgccgtcaat gatccccggg
     1981 gctgcgatac cgtctttgtg cagcttgaca gtccactcca gagccttacg aacctcgggg
     2041 gtatctaaag tcgccttgga gaaatcgtcg ctcatcaatg cgccgccggc ttgataaata
     2101 aatgccagcc agctatccat gctcgctttt ccgaattgca aagcatattt ggcgcccgaa
     2161 gccgcttggg ccttcttggc cgcctccacc atctgatccc aagtccaagc atccttggct
     2221 tttgtcggag gcttgatacc ggcttttgca aagtaatcgg cattataagc caccgcacga
     2281 accgaatccg cgaagggcaa cccgtatatc tgtccgtcat tgtaagtatc cgataacagg
     2341 cgcgctttgg aatacttatt caaaacactc ttgggaatgt atttgttacc agcctccaac
     2401 ttaccgacgg tcgcccattc accgacccgc tggggaacgg ccatggcgac atccggcaaa
     2461 tcattggcta tggcacgggt tttcactacc tcggcccatt tcgcctgggt cacaacttga
     2521 acattcagtt tgatattggg gtgctttttc tgataaaggt cgagactctt ctgaaccgat
     2581 gcccgaccgg cttcggtaac gtaatcgtaa agtacagata aggttatttg tttcggtgcc
     2641 gccagtgtag tggcgaataa acttccaacc aaggaaatac ataacactga caaaacagtt
     2701 actttgcgga tctgcattct ttacaatcct cctttttaaa agaaaaccgt cttgatgaaa
     2761 gccgatacag tacggtccga ttggcacctc ctttgatcga gccttgattc cctataagcg
     2821 cttagtcgtc tgctcataag tttaatcgat tgataacatt ttcaatttat cacattacgg
     2881 tacgaaagtc tgccgactgt ggtactatac tttgaatatc gcggtactgg gaacggtact
     2941 tttgtaccgg taccaaaacg gttagtcgag gaaacaagcg aggatgcgga taatataaaa
     3001 cggattaatc caggcccgcc tcagcagcca tccgggaggc ttgaacccga tcctttactc
     3061 ccatcttcga atagatgata ctcacgtagt tcttgaccgt ctgctcggca atataaagcc
     3121 gtttggcgat ttctttattg tcacaaccct tggcgagtaa acgcaggata tctttctccc
     3181 ggttgctcaa ttctttcagc cagacgggag actgaggaga aagaggcagt tgatcggtat
     3241 ccccctgatt tacctggacc agtttttcta ctaatttgga ggcaaccttg ggcgagatca
     3301 tcactcctcc ctgataaaca gccctgatgg tcgagatgag atcggtaggt ggaacatcct
     3361 tcagtaagta tccggccgca ccgaggcgta aagcttcaac cacgtattca tcatcgtcaa
     3421 aggtcgtcaa catcaatact ttcacttggg ggaatcgctc cttcagcatc cgggtgcatt
     3481 caaccccgtt catctccggc atccggacat ccattaagac cacatccggt tcctcagccg
     3541 aagccaattc cagggcctgc cggccgttga gcgccactcc caacacctgg atgtcttcgg
     3601 cacgggtctc caggaccgtt cgcaagcttt ccacgaataa catctgatca tccaccaata
     3661 atactttgat ctccttcacc agctcaacct tctttcaaag gaatccacgc cgataataca
     3721 aatccttttc ccgcttgatt attggcggag agttctccgc caagcttttc gatgcgttcc
     3781 ctcatgccca gcaaaccgaa gcctccctgg gaggagtttt ccataccctt accgttatcc
     3841 cggatattga tccgcacccc gttacgttct tgataaaagg agatctcaat caaggtggcc
     3901 ttaccgtgac ggagcgcgtt ggtcatccct tcctgtacca gacggtacgc ggcccaatcg
     3961 gcttcctcgc ccagaatcaa gggggcatcg cccagattca gctctacttt aatccgggtg
     4021 gccttggtaa aggtttggat taaatgataa atggcggtga ggccgttttc ttcctccaaa
     4081 tgtacagggc gcaatgcctg gagggcgctt ctcacgccca ccagcccctc cttggcttga
     4141 tcacgggcta gccgaaggtg attcaacagt tcctcccgat tcccggagga taagtcgagg
     4201 gacgcctcca gcatcatcac cagattggtc agggtatacg ccaacgtatc gtgcatctcc
     4261 cgggcgaggc gtttccgttc gttggatatc gcctcctgct ccgccatgac cgcgtaatcc
     4321 tggagctcca gattggcctg ggctagttga agcgtagctt cattcaggta ccggttgagt
     4381 tccttcgatg aaatctgatt atcccgttga aaacggatca tggcgctcaa ggtagtgagc
     4441 atggcaacaa acatggcaaa ggaaactcga tcatgtggag tcgcgacggg caagatcatg
     4501 ccccaggcca tcatcggcct ttggatcgtg atcaaaactc cggtgacaag tatggtaaaa
     4561 actaaaccga accgcaagga tgtatatgca aacgtctcga tgatgagcgc cccgaataag
     4621 gtcaactcga tcccaaggta actgccgaag ggaaatccca gaagtaccaa gattccggct
     4681 cttacc
//
LOCUS       DASVMB010000374         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_270484,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000374 DASVMB010000000
VERSION     DASVMB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270484"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VF531_10665"
     CDS             <1..930
                     /locus_tag="VF531_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7714471.1"
                     /translation="RQMGLKNLVLNNQTRNDLNFSSLALGGLTRGATPLELTAAYTPL
                     AGQGIYSEPIGVLEVRDSDGNVVFSDHSHKQVAIPEATAYVVTDMLRGVIMRGTGRGA
                     MIDRPAAGKTGTTSDYTNAWFVGYTPDMVATVWIGNDSQRIPVQLNGATIGSAKAAEI
                     WGNFMRKALSQTPVSDFIPPAGVVSGVEICTESGQLATPNCPETYYETYLAGTEPNQP
                     CSIHQDGALNPDNLATGAPDPGVTPAPVSDQPNATGTNAQTTTGKKKIVVVKICSASG
                     LLATPYCPESSVTTETFTAGEEPTALCNIHTAN"
     gene            complement(1110..>3013)
                     /locus_tag="VF531_10670"
     CDS             complement(1110..>3013)
                     /locus_tag="VF531_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX7714472.1"
                     /translation="MKVIQNRIHDKLDSLIHSSDNSKYLQELLVTVRGDRYVIPVKQE
                     YRSLFPGIVHDQSASGATLFIEPIAVVEMSNQLRIAQTEEVEEIGRILDELSGKVKDV
                     AENLLISVGILARIDLAFAKGRYSLALKASEPVLNADYSIDLIEARHPLLSGKVVPIR
                     VNLGRNFDTLVITGPNTGGKTVTLKTVGLLSLMAQSGLHIPAAPGSSLSVFEEICCDI
                     GDEQSIAQNLSTFSSHLTQIIKIIAAVHGPNALVLLDELGAGTDPTEGAALAMSILTH
                     LHRVGARTVATTHYSELKAFAYQTPGIENAAMEFDIDTLQPTYHLLIGLPGSSQAFAI
                     AQKLGLPDSIIMQARGYISAEAAKVDEMLRDIEIDRRKARENQIASEAARAKGETFQN
                     QYQTALTKFNQEKAELLRQARAEARQVLLETRRESENLLRRLREASPDELTEIVNQAR
                     QRITHDLDELDETPAVAEQNHVTDPGSLKPGSKVRVLSLGQNGVVLELNSDNALVQMG
                     MIRANLPYRDLERLPEADSSRERMKSVPVTRGKGTAPGLETAKNISAEINLRGLTVDE
                     GLYQLEKYLDQAILAGLHQIRVIHGKGTGALRQAVQQYLRDNPAVRNVAFAQQNEGGL
                     GATVAELRN"
BASE COUNT          713 a          814 c          750 g          736 t
ORIGIN      
        1 cgtcagatgg gactcaaaaa cttggtgctg aataaccaaa ctcgcaatga tctaaacttc
       61 tcctctctgg cattgggagg attgacccgc ggggctactc cgctcgaatt gacggccgct
      121 tatacgccac tcgccggaca aggcatttac tccgaaccaa ttggggtttt ggaagtccgg
      181 gattctgacg gtaacgtggt tttctccgat cattcccata agcaagttgc gatacctgag
      241 gcaactgcct atgtagtcac ggatatgttg cgaggcgtta ttatgagagg taccggccgg
      301 ggtgccatga ttgaccgacc tgcagcaggc aaaaccggaa cgaccagtga ttatacgaat
      361 gcctggtttg taggttatac acccgatatg gtagccacgg tttggatcgg caatgattcg
      421 caacgaattc cggttcaact gaacggagca accatcggca gcgccaaagc ggcagagatt
      481 tgggggaact ttatgcgtaa ggcactcagt cagacccccg tttccgattt catccctcca
      541 gccggagttg tctccggagt cgagatctgt accgaaagtg ggcagttggc cactccaaac
      601 tgtcctgaga cttattatga aacctatttg gccggcaccg aaccaaatca gccttgttcg
      661 attcatcaag acggagcttt gaatccagac aatttggcaa caggtgcacc ggatccggga
      721 gtaaccccgg caccagtatc tgatcaacca aacgcaacag ggactaatgc ccaaaccact
      781 acaggaaaaa agaagattgt agtcgttaaa atctgttccg ccagtggcct tttggctaca
      841 ccctattgtc cggagagcag tgtgaccacg gaaaccttca ccgcaggtga ggaaccgaca
      901 gctctatgca acatccatac ggcgaattga ggcgtgaggc atgagtatgt aagtcattta
      961 ttcttgtcga aagtcaatga attctatctg aaatctaagc ctaaaagtaa ctcacccctg
     1021 acccctctct tttcaaaaag agaggggcgt agggttatac tgaaagctca tgagtaccgc
     1081 ggggtgagtt gactcacccc tttcccctat taattcctca actccgccac ggtggcgccc
     1141 agtccgcctt cattctgctg ggcaaaggcc acgttccgga cggccggatt atcccgcagg
     1201 tattgttgga ctgcttgacg caacgcaccg gtacctttac cgtgaatcac ccggatctgg
     1261 tgtagtccgg ccaaaattgc ctggtccaaa tacttttcca gctgataaag gccttcatct
     1321 accgttaatc cgcgcagatt gatctcggcc gagatgttct tggccgtttc cagtccaggg
     1381 gcagttcctt taccacgggt aactggaacg gatttcatcc gttccctgga agaatctgct
     1441 tcaggcaacc gctccaaatc ccgataaggc aggttggcgc gtatcatgcc catctgcacc
     1501 aaggcgttat cactgttgag ttcgagaaca acaccatttt gacctagtga aagcacccgc
     1561 accttgcttc cgggtttcag ggaaccggga tcggtcacat ggttttgctc ggccaccgcc
     1621 ggggtttcat ccaattcgtc cagatcatga gtgatgcgtt gccgggcctg gtttacaatc
     1681 tcggtaagct catcagggga cgcctctctg agcctgcgaa gcaggttctc gctttcacgc
     1741 cgggtttcca gtaaaacctg ccgtgcctcc gcccgggcct gacggagtag ttccgccttt
     1801 tcttgattaa atttggtcaa agcggtctga tattgatttt gaaatgtttc gcccttggcc
     1861 cgcgccgctt cgctggcaat ctgattctca cgggccttac gacgatcgat ctcgatatcc
     1921 cgcaacatct cgtcgacttt ggcggcttcg gccgagatat agccccgcgc ttgcatgata
     1981 atggaatcgg gtaatccaag tttctgggcg atggcaaaag cctggctgct tccggggagg
     2041 ccgatcagca aatgataggt cggttgcaag gtgtcaatat cgaattccat ggctgcattc
     2101 tcgatccccg gtgtttgata ggcgaaagcc tttaactcgc tgtaatgtgt ggtggctacc
     2161 gttcgcgctc cgacacggtg cagatgagtc aggatgctca tggccagtgc cgccccttcg
     2221 gtgggatcag taccggctcc caactcatcc aacaacacca aagcgttcgg gccgtggacc
     2281 gctgcaataa tcttgatgat ttgggtcaga tgcgaagaaa aggttgagag attctgggcg
     2341 atactctgct catccccgat atcgcaacag atctcttcaa agaccgataa tgacgatccc
     2401 ggcgctgccg gaatatgcaa tcccgactgg gccatcaaag atagaagccc cacagtcttt
     2461 agggtcacgg tcttccctcc ggtatttggc ccggtgatca ccagtgtatc gaaattcctc
     2521 cccaaattga cccggatagg caccaccttc ccgctgagca acgggtggcg cgcttcgatc
     2581 aagtcaatgg aataatccgc attaagcacc ggttcgctgg cttttaatgc caaactgtaa
     2641 cggcctttgg caaaggccag atcaatacgg gccaaaatcc cgacactgat caacaaattt
     2701 tccgcaacat ctttgacttt tccagaaagt tcgtcgagga tccgcccaat ttcctctact
     2761 tcctcggttt gggcaattcg taactgatta ctcatctcca ctacggcgat gggttcaatg
     2821 aaaagtgtag caccactggc cgactgatcg tgtacgattc cggggaacaa tgaccgatat
     2881 tcctgtttca ctggaataac atagcgatca cccctgacgg ttaccaggag ttcctggaga
     2941 tatttagagt tatcactgga atgaatcaac gagtccaatt tgtcgtgaat ccggttttgg
     3001 atcaccttca tct
//
LOCUS       DASVMB010000375         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_637223,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000375 DASVMB010000000
VERSION     DASVMB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637223"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF531_10675"
     CDS             complement(<1..443)
                     /locus_tag="VF531_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7714473.1"
                     /translation="MAIKEILLLGNPKLYEVSSAIERKELEGIKPVVQDLHDTLLDFR
                     RKYGAGRAIAAPQIGVMKRLIYMYVDQPVVFINPVIDMKSPELMEVWDDCMSFPELLV
                     KVRRHKQCRIKFKDMDWSDREMMLEGDFSELLQHEFDHLDGILAVS"
     gene            770..1543
                     /locus_tag="VF531_10680"
     CDS             770..1543
                     /locus_tag="VF531_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7714474.1"
                     /translation="MTVCCMDVYPQKNDVYVGGNSVNFAFQCIKKGVSATSVLGCVGT
                     DDYGWVIIDILTKNRINVSHLHIREGRTASNQIYISRKGERFFLPDSWDGGVYQSFLL
                     SQEDWDFVLEHEIVAIPANNPNFPTSLEKLGSSGKLVVDFLDSRDLQLIEATLPRVAL
                     GFISGDHEMVARLKPLSEKITTPMIITLGAEGSIALHHGKELREAAVPVEAIIDTTGC
                     GDSYQAAFTIAWWQEHNLSKAMKEGATAAAGCLSHMGGI"
     gene            complement(1540..>2718)
                     /locus_tag="VF531_10685"
     CDS             complement(1540..>2718)
                     /locus_tag="VF531_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7714475.1"
                     /translation="VAGLRATASHKPLMENVPEQDATVVARLRSAGVIILGKTNLPEL
                     ALDSQTNSPVFGRTNNPWDPERTPGGSTGGGAAAVAAGISFLELGNDFMGSLRIPAHF
                     CGIYGLVPTTGLIPKKGFIPQPPPGGTFGQMLRMGMLSRSVEDLRIGLSVVAGQGPDE
                     PETVPVNWSGVSQEGSIIRIAWTDDLNTPVSEETCRVIRDFAQKLNTHGFIVERVNPD
                     FFDFDLSRRVFLRLFYSVMGTRMQPAVRWLVRVFGSPYLDMNLSKYLAAEAARIRLIN
                     DLESFFKRWDVLLCPVTATPAFRHLRPDRYQGPNPIYTKGIEVDGKRVPYATANLAMT
                     IPFSLTGHPVVTVPVGFSNEGLPIGVQVVGRRWNDAGLLDVAARLAKVSGGWRMPQIV
                     "
BASE COUNT          738 a          728 c          612 g          640 t
ORIGIN      
        1 gataccgcaa ggataccgtc cagatgatcg aattcatgct ggagtaattc ggaaaagtct
       61 ccttccagca tcatctcccg gtcactccag tccatatctt taaacttaat cctgcattgc
      121 ttatgacgcc tcaccttgac cagcagctcg gggaaggaca tgcaatcatc ccagacttcc
      181 atcagctccg ggctcttcat atcaattacc ggattgataa ataccaccgg ttgatcaaca
      241 tacatataga tcagccgctt catcaccccg atctgcggtg cggcaatagc ccgtcctgcc
      301 ccgtactttc ttcgaaagtc cagtagagta tcatggagat cctgaacaac aggctttatc
      361 ccctcaagct ccttccgttc aattgcagaa cttacttcat acagtttcgg gtttcccaat
      421 aataagattt ctttgatcgc caatcttatc acttcgcttc ctgaatcgtt tagataatgt
      481 tgtaaaaagt atatcaagaa aatgaatcca aatcaaaaaa gttttgacat acaacttctt
      541 aacaaaccat tattctccgt aaaaagctat gcatcgggat agaaatgaat aaagcaaaat
      601 atgataatca aacggcaatt catgaaaaga tttcaagtat atcccgcggt ataatttgat
      661 taaacacacc aaatcaaata cagaggaata cacgatgact cccaacgcct aaagtaaccc
      721 gtccattata cgggacagct ccaaaacgca agatttaaga atcgttgcaa tgaccgtttg
      781 ttgcatggat gtttatccgc agaaaaacga tgtctatgta ggcggtaact ccgttaattt
      841 tgccttccag tgtataaaaa aaggggtcag tgcaacttcc gttttaggat gcgtcggcac
      901 ggacgactat ggatgggtga taatcgatat tctcaccaaa aacaggatca acgtatctca
      961 tctccatatc cgcgaaggaa gaactgcctc gaaccagata tacatctccc gaaagggcga
     1021 acgttttttt cttcccgaca gttgggatgg gggcgtatat caatcattcc ttctttccca
     1081 ggaagactgg gatttcgttc tggaacacga gatcgtcgcg atacccgcca ataatcccaa
     1141 tttcccaacg tccctggaaa aattggggag cagcggcaag ctggttgtgg attttctgga
     1201 ttcccgcgat ctccaactga ttgaagcgac tttaccccgg gtcgctttgg ggttcatcag
     1261 cggtgaccac gagatggtag cccggttaaa acctttatct gaaaagatca ctaccccgat
     1321 gatcataacc ttaggagccg aagggagcat cgctttacat cacggcaaag aactacgcga
     1381 agcagccgtc ccggttgaag ccattattga taccacgggt tgcggcgact cttatcaagc
     1441 cgcatttacc atagcgtggt ggcaggagca taacctttca aaggcaatga aagaaggggc
     1501 taccgcagct gccggctgtc tgtctcatat gggaggtatt taaactatct gcggcattct
     1561 ccatcccccg ctcaccttcg ccaaacgggc ggcgacatcc aacagtcctg catcattcca
     1621 gcgtctcccc accacttgca cgccgatggg caggccttcg tttgagaaac ctacaggaac
     1681 cgttaccacc ggatgtccgg tgagactaaa aggaattgtc atcgcaagat tggcggtggc
     1741 gtaaggaacc ctcttgccgt ctacctcgat tcctttggta tagatggggt tcgggccctg
     1801 atatctatcg ggccgaagat gcctaaaggc gggagttgct gttaccggac aaagtaacac
     1861 atcccaccgc ttgaaaaagc tctctaaatc gttgatcaaa cgaatccgtg ctgcctcggc
     1921 cgccagatat tttgaaagat tcatatcgag atatggcgag ccaaataccc ggacaagcca
     1981 tcgtaccgcc ggctgcatcc gggttcccat caccgaataa aaaaggcgga gaaacaccct
     2041 gcgtgacagg tcaaaatcga aaaaatccgg attaactcgc tccactataa aaccatgggt
     2101 atttaacttt tgggcaaaat cccggatcac ccggcaggtt tcctcggata ccggagtatt
     2161 caaatcatcg gtccaagcga ttcgaataat tgagccttcc tgactcaccc cggaccaatt
     2221 taccggaacc gtttcgggtt catccggccc ctgcccggcc acgaccgaaa ggccgattcg
     2281 aagatcctcc accgaacggg agagcatacc catgcgtaac atttgaccaa aagtgcctcc
     2341 tgggggtggt tggggaataa aacctttctt ggggataagc cctgtggtag ggaccaaacc
     2401 gtaaatcccg caaaaatgag ccgggatccg gagcgatccc ataaaatcat tgcccaactc
     2461 taagaatgaa atcccggcgg caacagcggc cgcccccccg ccggtgctgc cgcccggagt
     2521 tcgttccgga tcccagggat tattcgttcg gccaaatacc gggctattgg tctgggaatc
     2581 aagcgccagt tccggcaggt tggtcttccc gagaatgatg actccggcac tccgcagccg
     2641 tgcgactacc gttgcatcct gctcaggaac attctccatc aatggtttat gactggccgt
     2701 cgcacggaga ccggctac
//
LOCUS       DASVMB010000376         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_562437,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000376 DASVMB010000000
VERSION     DASVMB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562437"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VF531_10690"
     CDS             complement(<1..764)
                     /locus_tag="VF531_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7714476.1"
                     /translation="MSHTITLIPGDGTGPELTAAARRVIEATGVKIDWEIHDAGIDVL
                     DKYGTPLPEHVLESIRRNKVALKGPITTPIGTGFRSVNVALRHALDLYACVRPCKSYP
                     GVRSRYQDIDLVIVRENSEDLYAGIEFDLGTPEAGQIISMAGGKIKADAAISIKPLSV
                     SGCRRIIKYAFEYAVRNGRRKVTAIAKANIMKFTDGLFYRVGREVAKEYEGRIAYEEV
                     LVDAMCMQLVQRPEGYDVLVLPNLYGDILSDLCAGLV"
     gene            complement(940..1968)
                     /gene="holA"
                     /locus_tag="VF531_10695"
     CDS             complement(940..1968)
                     /gene="holA"
                     /locus_tag="VF531_10695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7714477.1"
                     /translation="MNRAEFIKQMNEGRLLQTYLLLGEEKLFQEELLGAALRKLVAEA
                     DREFNFLRLNAATVSPEDLLSNLETPAFFGSGARIISLEDFEKAAAGVDEAAVKGLTR
                     LAGGVYFFISAVKLDGRKKLHQELQRRVLAVDCNKLRPADLPLWIKQRSEHMGLKLTS
                     DQLRLIAERLGTDLLLVRTQLEKVSTFCGKASSITDDDLDRLITGTPEADIFGLIDAV
                     SAKNPKLSLGKLETYLDSGENELKLLATLARQFRNITAALVAKEQRLTPRNLAERLGI
                     NPYVAEKSFSQAGRFDLEELHGILERLLRADYAIKTGQREPRLELELAVVEITSGTLI
                     RPFPRMYE"
     gene            complement(2020..>3795)
                     /locus_tag="VF531_10700"
     CDS             complement(2020..>3795)
                     /locus_tag="VF531_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX7714478.1"
                     /translation="TMSEGIVIIRLVDQMKRTGTIHLISVSGLHVGLVVLTLLALCRF
                     FRIPERYRILPVTLGIWFYILMTGMETPVLRAGLMIQLYLLGKLLKTQDEPVNRLSLA
                     GLIILFLKPVNLFEIGFQLSFLATLGIIWLYPLLRTWLHPARLHRSTMGSKPAGGRSW
                     PEFLVKPCRWVWEGFLASLAAQVMITPVIVHYYQTITWSSPIVNLFLLLPTELMVIGG
                     LAGEFLGLGLAWCGRLVLVGVDWTTSLVRYILAFWAGQPWAASWSPRWPWPWFAGYYL
                     AMLLLLDALRPNRLTGKRHWNPGPVFIGVLIALNLLTWTALLYRREQTYLRVAFIDVD
                     QGDSIFVRTPAGINVLIDGGDKGKGRDRVLPFLQANGIERLDYIFASHGHWDHLGGLV
                     EVVQELQVGRIYLTNEPDNPALQSLKIGIQNRYSQKMRVNQGFKLRLDSSVSLEVLLL
                     PDPGNENDRSMVLLFGYGKNKLLLTGDLSVEGEEILQRKYPAALRATVLKVAHHGSNQ
                     ASGLRFLTQVRPLLAVISVGEGNRYGHPGSRTLNRLRSLGVRVYRTDQFGTVDLKVYP
                     ERIRVRCESPPGFASDGRALSTATR"
BASE COUNT          905 a         1136 c          933 g          821 t
ORIGIN      
        1 acgagaccgg cgcagaggtc ggagaggata tccccgtaaa gattgggcag gaccagtaca
       61 tcgtagcctt cgggccgttg caccaattgc atgcacatcg cgtccaccag gacttcctca
      121 taggcgatcc ttccctcata ttccttggct acctcacgcc ccacccggta aaagagacca
      181 tcggtaaatt tcatgatgtt ggccttggcg atggccgtca ccttacggcg accgttacga
      241 acagcgtact caaaagcgta cttgatgatg cgacggcagc ccgagacgga aagcggcttg
      301 atggagatgg cggcatccgc cttgatctta ccccctgcca tggagatgat ctgtcctgct
      361 tccggggttc ccaggtcgaa ctcgatcccg gcatacagat cctcactgtt ctcacggaca
      421 atgaccaggt caatatcctg ataacgggag cgtactcccg gataggactt gcacggccgg
      481 acacaggcgt acagatccaa tgcatgccgc aaggccacat tgacactgcg gaaccctgtg
      541 ccgataggcg tggtgatcgg cccctttaga gccaccttgt tgcggcgaat cgattctaga
      601 acatgttcag gcaatggagt gccgtactta tcgagcacat cgatcccggc atcatggatc
      661 tcccagtcga tctttacccc cgtggcttca atcacccggc gggctgcagc ggttaactca
      721 ggaccggtgc cgtcgccggg aatcagtgtg atagtatgtg acaaaacaga ttctccttcc
      781 aatggcttta taaaatgaac catccaaaat tcgacagccc gtcacccaaa ctcctgccgg
      841 tttcatatga atacagaata ctcgttggaa ggagaaaaaa taccagaccc ctccctttgg
      901 ggttgggtaa atatgtggtc cctccccgct gccatacttt cactcataca tccgtgggaa
      961 agggcggatg agtgtccccg atgtgatctc caccaccgcc aactccagct ccagtcgcgg
     1021 ttcccgttgg ccggtcttga tcgcataatc cgcccgaagc aaccgttcca ggattccatg
     1081 aagctcttcc agatcaaacc gccccgcctg tgaaaaactc ttctccgcca catacggatt
     1141 gatccccagt cgctccgcca ggttcctggg tgtcagtctc tgctccttgg caaccaatgc
     1201 cgctgtgata ttccgaaact gccgcgccag agtcgccagg agctttagct cgttctcccc
     1261 cgaatcgaga tacgtttcaa gtttccccaa gctcaacttg gggtttttcg cggaaaccgc
     1321 atcgatcaat ccgaaaatgt cggcttccgg cgtgccggtg attaaccgat ccaaatcgtc
     1381 atcggtgata gaggaagctt tgccgcaaaa agtactgacc ttctcaagct gggtccggac
     1441 gaggagcagg tcggtaccga gccgttcggc aataaggcgg agctgatcgg aggtgagttt
     1501 caaccccata tgttcggagc gttgcttgat ccagagcggc aggtcggcag gtcgcagttt
     1561 attgcagtcc acggccaata cccggcgttg cagctcctga tggagttttt tcctcccgtc
     1621 aagttttacg gcggaaatga aaaagtaaac cccgccggca agtctggtca agcctttgac
     1681 tgccgcctca tccaccccgg ccgcggcctt ttcgaaatcc tccagggata tgatccgggc
     1741 cccgcttcca aaaaaagccg gggtttccag attgctcaac agatcctccg gcgaaaccgt
     1801 ggctgcattc aacctcaaaa aattaaactc ccgatccgcc tctgccacca attttcggag
     1861 cgcggcgccc aggagttcct cctggaacaa tttctcctca cccaagagca ggtacgtctg
     1921 gagcaaacgt ccctcattca tttgtttgat aaactccgcc cgattcatcg gcttcccctt
     1981 tcatacattg atgtatcgtt ttcacgaatg ggttcttcct caccgtgtag cggtagagag
     2041 tgcgcgtcca tcagacgcga agccaggggg tgattcacac cttaccctta tcctctccgg
     2101 gtaaaccttc agatcaaccg ttccaaactg atcggtccga tatacccgca cccccagaga
     2161 ccttagccgg ttcaatgtcc tgctgccggg atggccgtaa cggtttccct cgcccaccga
     2221 gatcactgcc agtaacggcc ggacctgagt caaaaatctc aaaccgctcg cctgattgct
     2281 cccatgatgc gccaccttta gcaccgtggc ccgaagcgcg gccggatact tccgttgcaa
     2341 gatctcttcg ccctctacgc ttaaatctcc tgttaacagc agtttatttt tcccataacc
     2401 aaacaggagc accatcgatc ggtcgttctc attaccggga tcgggaagta acagaacctc
     2461 cagggatacg gaactgtcca aacggagttt aaacccctga tttactctca tcttttggga
     2521 gtaccgattc tgaataccaa tcttcaagct ttgcagcgcg ggattgtcgg gttcgtttgt
     2581 caaatagatg cgacccactt gaagctcttg aaccacctct acgagtcccc ccaagtggtc
     2641 ccaatggcca tggctggcga atatgtaatc cagccgttct atgccgtttg cctgtaaaaa
     2701 aggcagcact ctgtcccttc ccttgccttt gtctccgccg tcgatcaata cgtttatccc
     2761 cgccggagtc cggacaaaga tcgaatctcc ttggtctacg tcaataaagg ccacccttag
     2821 gtatgtttgc tcccgccggt aaagtaaggc cgtccaggtg agcaagttca gcgcgattaa
     2881 gactccgatg aacaccggcc ccggattcca atgtctctta ccggttaggc ggttaggccg
     2941 caaagcgtca agtaggagga gcatggcgag atagtaaccg gcaaaccaag gccacggcca
     3001 gcggggtgac catgaagccg cccagggctg gccggcccaa aaggcaagaa tatagcggac
     3061 gagactcgtg gtccaatcta ccccgactaa taccaaccgg ccgcaccaag ccagaccaag
     3121 accgaggaat tccccggcga gtccgccgat gaccattagc tccgtcggaa gtaataaaaa
     3181 taagtttacg atcgggcttg accaagtgat cgtctgataa tagtgaacga ttaccggtgt
     3241 aatcataacc tgagccgcaa gtgaggccag gaaaccctcc catacccagc ggcacggttt
     3301 caccaaaaac tccggccaag accgcccgcc cgccggtttt gatcccatag tgctccggtg
     3361 aagccgggcc ggatgcaacc aggtcctcag cagcggatag agccagatga tccccagggt
     3421 tgcaagaaac gaaagctgga agccgatctc aaacagattg accggcttta gaaaaagtat
     3481 gatcaacccc gccaaactga gccggtttac cggctcatcc tgagtcttca gcagtttacc
     3541 caggagatac agctggatca tcaaccccgc ccggagtacc ggtgtttcca tcccggtcat
     3601 caggatgtaa aaccagattc ccagggttac gggaagaatc cggtaacgtt ccggaatacg
     3661 gaagaaacgg cagagcgcca gcagagtcag gaccaccaag cctacatgta gaccggaaac
     3721 cgagatcaaa tgaatggttc cggtacgctt catctggtcc accagccgga tgatgacaat
     3781 accttcactc atggt
//
LOCUS       DASVMB010000377         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_270870,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000377 DASVMB010000000
VERSION     DASVMB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270870"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VF531_10705"
     CDS             complement(<1..779)
                     /locus_tag="VF531_10705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7714479.1"
                     /translation="MQPKNDTAASSGEQTYLASDAYQTRLDADKTQLAAEGYHTILAV
                     QPQPVTAGAAATKEEQELYRIGDYIGDRYEVLAIHRGAWGVIYVTYDHQTELPRALKT
                     IQKRYRFDKQLLELFREEAALWVGLEKHPYIVRAYSVEEYEDQPFVITEYISNPQGND
                     LRSWLGSSKLTLAVGVEMALKIAQGMQYAHHKVAGLVHRDLKPANILVDEYAQPYITD
                     FGLVRAAGSDAGTPAYMAPEQWRKEPVTVQTDIYAFGCILY"
     gene            complement(860..1345)
                     /locus_tag="VF531_10710"
     CDS             complement(860..1345)
                     /locus_tag="VF531_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714480.1"
                     /translation="MKPVREIYERIAKIDWFSNCGKEINDIGISYEPMKDWKTAVQKC
                     KSNVWESVQLEARNILTVALSNNWPNEDRLWNEITDQAKELLNIGVIPVITEFVNHRQ
                     LDIAVLHSVRWDTLAAMMEHAYSPYVKPGFYTELLKVYEVGHFPCGWKGKWPDGTLLI
                     Y"
     gene            complement(1722..1988)
                     /locus_tag="VF531_10715"
     CDS             complement(1722..1988)
                     /locus_tag="VF531_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714481.1"
                     /translation="MSESKVCLLRNIHNVEDGVEGEVYFKLFNQYIKLYSWKGAGMNY
                     IEKCANYLNSFSDEVINRLCEASIRYCNDFCDMVGEEPKEWSYM"
     gene            complement(2025..3026)
                     /locus_tag="VF531_10720"
     CDS             complement(2025..3026)
                     /locus_tag="VF531_10720"
                     /inference="COORDINATES: protein motif:HMM:NF042574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6709 family protein"
                     /protein_id="HEX7714482.1"
                     /translation="MLQDGFVLKQMQKYNRRLFIITIVLICLAVTSGNELVKYWHNLQ
                     KGPFEITARQLNRIQSVDSLREYYVKVRGQKLYRLSKSHDYYLLEINHAFLVVKGKVG
                     EDSLEFSGELKDLPDDERKKLDQALGVMNGEGGKPKVSIHPVFLEAGELQTRESTDYW
                     LFGALLVLVIRNIFVLLRRHSDPSLHPSCKYLSKLGSVETIVINIDSEVSKAEGAFGK
                     ASLIVTDSWIIVKKFFKLQFLALSDVVWAYQKVTSHRYNLIPVGKTHELIVYTKDQEV
                     HEFKVKNGLTGEEAVELIGSNAPWAFTGYSEKLETMWKKNRPDFIQAVQERSGNSGR"
     gene            complement(3048..3755)
                     /locus_tag="VF531_10725"
     CDS             complement(3048..3755)
                     /locus_tag="VF531_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714483.1"
                     /translation="MGKIINFKIKLWLIILGALAVVIAIGVIVDSIDQKANGPEQSFA
                     IYNSGDKEVSVTFHRPKQNNEYGGYLDDISVKPGETKMQDYPAGTYKVIVWDGSVTDD
                     NSEEFRSIKNFKVELPDGKDNINPIYIDTTGNYFALINVNYLYSGSALTNQIAESVGA
                     SGTDPVLKKLYNGKLPFRTLEQDTDQTIVGPSDKLPKEVSAGESVYILAPVPNTVKTN
                     DEVYQYALRFMETHMQQ"
     gene            complement(3916..4350)
                     /locus_tag="VF531_10730"
     CDS             complement(3916..4350)
                     /locus_tag="VF531_10730"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7714484.1"
                     /translation="MMAPLKLTCLSGMRDGEAVQVLLKDNASLAEANAVIEAILGRSE
                     DCTVSFPEDPELSRRHARLIWNAPNASWRLEDLKSTNGTYLGEFSQAKQIDEPVSLAY
                     GDVFRVGRTCLRLEPPLLRETETAAVAYAQAGDEPSRPVKPE"
     gene            complement(4547..>5136)
                     /locus_tag="VF531_10735"
     CDS             complement(4547..>5136)
                     /locus_tag="VF531_10735"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX7714485.1"
                     /translation="LAQINFEEVPDIGSFPSHGILQFYISPKDDVFGLNFDKPTDQAS
                     FRVIYIPEVIKDYAKLMNDFSFVCNDDEYFPVGAEGGLEFKVEHAPVGTGDFRFEKLL
                     NTSSYNLFDDQQADWYEEHYSSAGHKMAGYAYFTQSDPRDTSYQDYDILLLQMDSDDE
                     LNMMWGDSGVANFFIRPEDLKNLDFTKVLYNWDCC"
BASE COUNT         1297 a         1333 c         1037 g         1469 t
ORIGIN      
        1 tcgtaaagaa tgcatccgaa agcgtaaata tcggtttgga ctgttacggg ttccttccgc
       61 cactgctccg gagccatata ggcgggtgtg cccgcatcgg atccggcggc ccgaaccagg
      121 ccgaaatcgg tgatatacgg ctgggcgtat tcgtctacca gaatattggc cggttttaaa
      181 tcccgatgca caagtccggc tactttatga tgggcgtatt gcatgccttg agcaatcttc
      241 agcgccatct ccaccccgac cgccagggtt aacttggaac tccccagcca gcttcgtaag
      301 tcattgccct ggggattgct gatatattcg gtgattacaa aaggttgatc ttcatattcc
      361 tctacactat aagcccttac aatatacgga tgtttctcaa gcccgaccca aagagcggct
      421 tcctccctaa agagctccaa caattgctta tcaaaccgat atcttttttg gatcgtcttt
      481 aaggcccggg gaagttccgt ttgatgatca taggtcacat agatcactcc ccaggcgccc
      541 cgatggatcg ccagcacttc gtaacggtcg ccgatatagt cgccgatccg gtagagttct
      601 tgttcttcct tagttgccgc cgctccagcc gttacgggct gaggttgcac cgccagtatc
      661 gtatgataac cttccgcggc cagctgagtt ttgtccgcat ccagcctggt ttgataagca
      721 tcgcttgcaa gatatgtttg ctcccctgag gaagcggcag tatcattttt gggttgcatc
      781 acggtccact cctaatccgt taaccatccg gctgcggttg acagaatata tctcacgctt
      841 tcatttcaag caaaatttac taatatatca gcaacgttcc gtcaggccat tttcctttcc
      901 aaccgcaagg aaaatgaccg acttcgtaca ctttcaataa ctcggtataa aaaccgggct
      961 ttacgtaagg actatatgca tgttccatca ttgcggcgag tgtgtcccat cggacactgt
     1021 gcaaaaccgc tatatcaagt tgacggtgat tcacgaattc cgtgatcacc ggtatcactc
     1081 ctatgttcaa caattctttg gcctgatcgg taatctcatt ccacaatcga tcctcattcg
     1141 gccaattgtt agataaggca acagttaaga tatttctagc ttcgagctgg acactttccc
     1201 aaacgttact cttacatttt tgaactgccg ttttccaatc cttcatcggc tcatagctga
     1261 tgccgatatc attgatttcc ttgccgcaat tggagaacca gtctattttg gcaatccgtt
     1321 cgtaaatttc ccggaccggc ttcattgatc aattcctccg tccgtcatgc ctgtgtacat
     1381 ctgagctaat atccatcaat gtgatattag ccctcatcac ttctcatcct ttcatgtgcc
     1441 ctagcaaata ctccggccta aaaccagatc ccctaccccc tttaaaaggg aaaaaagaat
     1501 gacttcgcgg gccaattcgg tttagctgct cccagctgct tttacaaaca ccggagcatg
     1561 gtggttcccc cttttaaagg ggccaagagg atctcagtta gaccaagccg ggaatatcct
     1621 gcctctgcac attttctgct gtatacttgc taacttttac gcgtaattcc attcatcttt
     1681 cgtagtaaaa tctccccagg gattttcacc ttcataagcg cctacatata ggaccattct
     1741 ttcggctctt cgccaaccat atcacagaaa tcattacaat accggatcga ggcctcgcac
     1801 aaccggttaa ttacttcatc actgaaggag tttaaataat tggcgcattt ttcaatatag
     1861 ttcattcccg cacccttcca ggaatatagt tttatatatt gattaaagag tttaaaataa
     1921 acctcccctt cgaccccatc ctctacattg tgaatattcc gcaataaaca aaccttactc
     1981 tcagacactt cattaccctt ccttcaatgg taaagatcaa ccattcatcg acccgaattg
     2041 cctgaccgtt cctgaaccgc ctggatgaaa tcgggccggt ttttcttcca catcgtttcc
     2101 aacttttccg aatatccggt aaaagcccaa ggcgcattgg agccaatcag ctccaccgct
     2161 tcttcgcccg ttaggccgtt tttaaccttg aactcgtgaa cttcctggtc cttggtataa
     2221 acgatcaact catgggtttt acccaccgga atcaagttgt accggtgtga agtcactttt
     2281 tgataggccc acacaacatc cgatagggca agaaactgga gcttaaaaaa cttcttcacg
     2341 ataatccagg aatcggtaac gatcagcgaa gctttcccga aagcgccttc ggctttactc
     2401 acctcgctgt caatgttaat cacgattgtc tccaccgaac cgagcttgga caggtatttg
     2461 caactcggat gtaaacttgg atcggaatgt cttctaagta atacaaaaat atttctgatg
     2521 acgagaacca gcaaagcccc aaacagccaa tagtcagtgc tttcacgggt ctggagctct
     2581 cctgcctcaa ggaataccgg gtgaatggat acctttggct ttccaccctc tccgttcatg
     2641 acgccgagtg cttgatcaag cttttttcgt tcatcatccg ggaggtcttt cagttcaccg
     2701 ctgaattcga ggctgtcttc accgactttg ccctttacca ccaaaaaagc atgattgatt
     2761 tccaataaat aataatcatg actttttgaa agccgataga gtttttggcc ccttacttta
     2821 acataatatt ccctaaggga atccacactt tgaatcctgt ttagctgacg ggccgtgatt
     2881 tcaaaagggc ccttttgcag attgtgccag tatttcacca attcgtttcc cgatgtaact
     2941 gccaggcaaa tcagcacgat cgtgatgatg aaaagtctgc gattatactt ctgcatttgt
     3001 ttcagaacaa aaccgtcttg taacatattg tattcccccc acagtctcta ctgttgcata
     3061 tgcgtttcca taaaacgcaa cgcatattga tatacctcat catttgtttt gacggtattg
     3121 ggcaccggag ccagaatata cacactttca ccggcgctaa cctctttggg gagtttatcg
     3181 ctgggtccca caattgtttg gtctgtgtcc tgttcaagtg tacggaacgg gagtttaccg
     3241 ttatacaatt tcttgagcac cggatccgtt ccggaggccc cgacggattc agcgatttga
     3301 ttggtcagtg ccgaaccgga gtagagatag ttgacgttta tcaatgcaaa ataatttccc
     3361 gtagtatcga tgtaaatggg gttgatatta tccttaccat cggggagttc cactttgaaa
     3421 tttttgatgc tccggaactc ctccgaatta tcatccgtga cgctgccgtc ccagacaatc
     3481 actttgtaag tgccggcagg ataatcctgc attttagtct cacccggttt tacggagatg
     3541 tcatccaggt aaccgccgta ctcgttgttc tgcttcgggc ggtgaaaggt gacgctgact
     3601 tccttatccc ccgaattata gatggcgaag gattgttccg gtccgttagc tttttgatcg
     3661 attgagtcga caatgacgcc gatggcaatc acaaccgcta aggcccctaa aataatcaac
     3721 catagtttaa tcttaaaatt gataattttc cccattgtag tcagtctcct tatgattttt
     3781 tgtttaaagc tattctttga tgcataatct cctgaaaact gcgaaggatt ggccgatacc
     3841 ctttaaagta cttccttttc agtcaaccct tcgagagaca gagctttatt aatattaaaa
     3901 cgatcacttt cttgcctatt ccggtttcac cggccgggaa ggttcgtccc cagcctgggc
     3961 ataagccacg gcggcagtct cagtctcccg taaaagcggc ggttccaaac gcagacaggt
     4021 acggccgacc cggaaaacat cgccataggc cagtgaaacc ggctcatcta tctgcttggc
     4081 ttgactaaac tcccccaggt aagtaccgtt ggtactcttt aaatcctcca atcgccagga
     4141 agcgtttggg gcgttccaga taagccgggc atgccgccgg gacaactccg gatcttccgg
     4201 gaaactgacc gtgcaatcct ccgagcgtcc cagaattgct tcgataactg cattagcttc
     4261 agctaatgat gcattatcct tcaacaaaac ctgaacggcc tcgccatccc gcataccgct
     4321 taggcaggtt aatttaagtg gtgccatcat cacttttcct cctcgtttca acctagaaaa
     4381 tgccaatgat gcttgtcgct caagtaagtt tactttaaac cacgaaacac acgaaaatca
     4441 cgaaaatgga atacgcagat ttggtttttt ttcgtgtatt tcgcgccttt cgtagttaaa
     4501 taggtcttta tactttcatg gttccaagaa tccgtgactg tatatatcag caacaatccc
     4561 agttatataa tactttggta aaatccaggt tttttaagtc ttccggcctg ataaagaagt
     4621 tggctacccc gctgtcgccc cacatcatat tgagttcatc gtcgctgtcc atttgcagta
     4681 acaaaatgtc ataatcctga tacgaggtat cccggggatc gctttgcgta aaataggcgt
     4741 atccggccat tttgtgtccg gcgcttgaat aatgctcttc ataccaatcc gcttgctgat
     4801 cgtcaaacaa attgtatgaa gaggtattca atagtttttc gaaccggaaa tccccggtcc
     4861 ctaccggagc atgctccact ttgaactcca gcccgccttc cgcccctacc gggaaatatt
     4921 catcatcatt acagacaaaa ctaaagtcgt tcatcagttt tgcgtaatcc ttgattactt
     4981 cagggatgta gataactcga aaacttgcct ggtcggtcgg tttgtcaaaa ttaaggccga
     5041 aaacatcatc cttcggtgag atatagaatt gcaaaatccc atgtgaagga aagctcccga
     5101 tgtcgggtac ttcttcaaaa ttgatttgag cgagta
//
LOCUS       DASVMB010000378         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_342023,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000378 DASVMB010000000
VERSION     DASVMB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342023"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VF531_10740"
     CDS             complement(<1..289)
                     /locus_tag="VF531_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX7714486.1"
                     /translation="MIGKTVDVNERLPLIQSIPLSLQHLFAMFGATVLVPYLVGLDTS
                     VTLFTSGIGTLLYILITKGKIPAYLGSSFAFITGLMAIIGAAPGNLGSPEKI"
     gene            complement(482..1036)
                     /gene="pyrR"
                     /locus_tag="VF531_10745"
     CDS             complement(482..1036)
                     /gene="pyrR"
                     /locus_tag="VF531_10745"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7714487.1"
                     /translation="MVEIKAQVMDEENIRRALYRLSHEITEHNKGTSDLILVGIRTRG
                     VPLANRLAEFIKEHENVQVPVGMLDITFYRDDLSLISSQPVIRRTELPLNISDQRVIL
                     VDDVLFTGRTVRAALDALMDLGRPSSIQLAVLIDRGHRELPIRADFVGKNVPTARREV
                     VQVRLKETDGEDRVVITEYEISPA"
     gene            complement(1309..2451)
                     /locus_tag="VF531_10750"
     CDS             complement(1309..2451)
                     /locus_tag="VF531_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7714488.1"
                     /translation="MAEVIIQECPGYDLQVLTAKLNEGMALLGGWGRFIRPGQKVLLK
                     VNLIGPKTSESAAVTHSEFARALTRILKREGCEVWIGDSSGGAIAGIAPTKRAFETSG
                     LQKIATEEGAILKNFDREGVVEITPPGKTNRLYLARPLFEADLVINLPKLKTHSAGVY
                     TGAVKNFFGCVPGLRKAAYHQAAPDPGDLGQVFSDIHRSVKAGLHIMDGVTAMDGAGP
                     TAGKVYPAHKILISTDPLALDTVAARMIGLGVEEIPTLRAAIADGLGVSDLNRIKLAG
                     DYHTIPMLHGFKIPRPRRTKGGSQFLIRLIDFLKTRPRIQTKICKNCNTCVDSCPVGA
                     IDRDSKRVDDTKCIECLCCHELCMYKAVRLKHDHWLAGLLTGFRED"
     gene            complement(2499..3317)
                     /locus_tag="VF531_10755"
     CDS             complement(2499..3317)
                     /locus_tag="VF531_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714489.1"
                     /translation="MEQTHRTILETAPARRTTGKRIILLICLILLVFLLLLYSCRNWL
                     VKTVVERVVRQNLGVTVNIGSLRLDPWHGDLAITDLIVYNPPGFDHANTPTTLARIPS
                     IRIQYDLPAFFQKNKLHLTYLELNLYEMSIIKNRRQEFNLNRIQSLAGGSAKQAASRQ
                     VSAEKPQGLEFQIDRLTLTATQLMYLDYSKAKPKTLRIKLGIYQERFTNLNSLDDLVR
                     VVAARVVAKAGLYNLGLPFRHLSQGVEKAGGRISSGLRKAAAGADHFLKGIIKK"
BASE COUNT          838 a          939 c          863 g          868 t
ORIGIN      
        1 cgatcttctc agggcttccc aaattacccg gagccgcgcc gatgatggcc atcaatcccg
       61 taatgaaagc gaaagacgat ccgagataag ccgggatttt gcctttggtg atgagaatgt
      121 aaagcagggt tccgataccg ctggtaaaga gggtcactga agtatccagt cccaccagat
      181 acggaactag caccgtcgct ccgaacattg cgaaaagatg ttgcaagctc aggggaatgc
      241 tctggattag cggcaatcgt tcattgacat caaccgtttt tccgatcatt tttttcctcc
      301 gttattcgta ttggttgctt cttatatcgt tgagggctgt cccaaaaaac agatcgtctc
      361 ctctccatgg gacaaaatgt ttttggggcg cctcagaagc cataaaaaaa ctcccaaaag
      421 ggaggatgat cgcgtttacc cgtcctccct tacctgcctc acggggcaag atttaaaggg
      481 atcaagctgg ggaaatttca tattcagtta ttactacgcg atcttcgccg tcagtctctt
      541 tcagcctgac ctgaaccact tcgcgacggg ccgtgggaac gttctttccg acaaaatccg
      601 cccggatcgg gagttcccgg tgaccccggt cgatcaacac cgccaactga atactgctgg
      661 gacgccccag atccatcaag gcgtccagcg ccgcccgaac cgttcttccg gtaaagagca
      721 cgtcgtctac caggattact cgctggtcgg agatgtttag cggaagttcg gtccggcgga
      781 ttaccggctg ggacgagatg agcgagagat catcccggta aaaagtgata tccaacattc
      841 ccacgggaac ctgaacattc tcatgttcct tgatgaactc cgctaaccgg ttggccagcg
      901 gcactccccg ggtccggata cctacgagga tcaggtccga ggtccccttg ttatgctcgg
      961 tgatctcatg ggagagccgg tacagcgccc gccggatatt ttcctcatcc attacttgag
     1021 ccttaatctc aaccatatca acacctccaa ccgttatttc acaggcaccc gcaatcagcc
     1081 ggagcaataa aaaaccttct tacgcaggcg taagaaggtg aatttctgca agttagcttt
     1141 cacggcgaaa ctcccggaaa gtaactgaaa cggaatgata tcgtgcacgc aaccttccta
     1201 gcctcacggg actagcatta aaggatcaat gttgaaatcc gtatccaatg tgatattaaa
     1261 ttactaatag cttaccagag ttcgaccggg atgtcaatgg atatcgtttt aatcttcccg
     1321 aaatccggtc aaaagacctg ccaaccaatg gtcgtgtttc aggcgaaccg ccttatacat
     1381 gcatagttcg tgacagcata ggcattcgat acattttgta tcatcaaccc gtttactgtc
     1441 gcggtcaatc gcgccaaccg gacagctgtc gacacaggta ttgcagttct tgcaaatttt
     1501 ggtttggatc cggggacggg tcttgagaaa atcgatcagc cggatcagaa attgggatcc
     1561 tcccttcgtc cgcctcggcc ggggaatttt gaaaccgtgt aacattggaa tcgtatgata
     1621 atccccggcc aatttgatcc gattcaaatc ggagactcct aagccgtcgg caattgctgc
     1681 ccgcagggtg ggaatctcct caaccccgag gccgatcatc ctggcagcca ccgtatccaa
     1741 ggctagcgga tcggtactga tcaatatctt gtgggccgga tacaccttcc cggcagtggg
     1801 accggctcca tccatggcgg ttactccatc catgatatgt aacccggcct tgaccgaacg
     1861 gtgaatatcg gagaagactt gccccaagtc tcccggatcc ggggccgcct gatgataggc
     1921 ggccttacgc aatcccggaa cacaaccaaa gaaattcttg accgctccgg tgtaaacccc
     1981 ggcgctatgc gtttttagtt tcggaagatt gatgaccaga tcagcctcga ataacggccg
     2041 cgccagatat aaccggtttg tttttccggg tggagttatt tcgaccacac cttcccggtc
     2101 gaagttcttt aaaatcgccc cttcttcggt ggcgattttt tgaagccccg atgtttcaaa
     2161 agcccgtttg gtaggcgcta ttccggctat cgcaccgccg gagctgtctc cgatccatac
     2221 ctcacacccc tcccgtttca ggatccgagt gagcgcccgg gcgaactctg aatgggtgac
     2281 tgccgctgat tccgacgtct tgggaccgat caggttgacc ttgagaagca ccttttgacc
     2341 cggacggata aaccgccccc acccacccag taaggccata ccttcgttca gcttagcagt
     2401 taagacctgt aaatcgtatc cggggcattc ctgaatgatc acttccgcca tcggcaagac
     2461 ctccgtttaa ataaccatgc agttcagtta agaaatgact attttttaat gattcccttg
     2521 agaaagtgat cggctccggc cgcagctttt ctcaaaccac tggagatcct gccaccggcc
     2581 ttctctaccc cttgggacaa atgcctgaag ggcaacccca ggttatacaa tcccgctttg
     2641 gccacaactc tcgcggcaac tacccggacc aaatcatcca gactgtttag attggtgaac
     2701 cgctcttggt agattcccag tttaatccgg agggttttgg gtttggcttt ggagtagtct
     2761 agatacatca gttgcgtggc ggtaagtgtc aggcggtcga tctgaaattc caatccttga
     2821 ggtttttccg cggagacctg acgggacgca gcctgtttcg cgcttccgcc ggccagggat
     2881 tgaatccggt ttagattgaa ctcctggcgc cggttcttga tgatactcat ctcatacagg
     2941 ttcaattcca gatatgtcag gtgtaacttg tttttttgaa agaaagcggg taagtcatat
     3001 tggatccgga tcgatgggat ccgggcaagg gttgtcggtg tattcgcgtg gtcgaaaccc
     3061 ggcgggttat agacgatcaa atcggtgatc gccaaatctc catgccaggg atccagccgc
     3121 aggctgccga tgttgacagt cactcccagg ttctgccgga cgacccgttc gactacggtt
     3181 ttaaccaacc agttccggca agaatataaa agcaacaaga aaaccaaaag aatcaggcaa
     3241 atcagcaaga taatccgttt tccggtggtc cggcgcgccg gggcggtttc gagaattgtc
     3301 cggtgggttt gttccattcg caattcctcc tttgcgccga tctttttcgc tacggaaacg
     3361 gtttttcctt tttttgccgg atttgaagtg aattttcatc atcaggacga aaaagtttga
     3421 caggcgggga aagaactgtt gcaatcgaat gatttacgcg ttatgctatg catggatcgg
     3481 tcgaaatacc ctcaagtgac aagttacg
//
LOCUS       DASVMB010000379         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_637428,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000379 DASVMB010000000
VERSION     DASVMB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637428"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VF531_10760"
     CDS             complement(<1..714)
                     /locus_tag="VF531_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714490.1"
                     /translation="MDRLNIIQKAVISIILATMVIAVVTPLLFFLNVAFSSDVEMSKF
                     PKSIIPKFTVTVKVAPAADGEYEIFYNKGEGYESIITTRNPIKLENHFKRQYSVTVPG
                     EKLLADFGKTRKKGLMEFTYVKNTFYNFNQFFAIVNNSLPALKNSILVCLYTILISLI
                     IGSMAGYAMARYSFKFKETINITLLIVRMFPVVGISIPMAILLIKFGLFDTLLGLALL
                     YSIPNVALTAWITSSIFVGI"
     gene            complement(715..1602)
                     /locus_tag="VF531_10765"
     CDS             complement(715..1602)
                     /locus_tag="VF531_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714491.1"
                     /translation="MINRKLSNSKKAIPYLLLLPAFIYYAVFWLTPVFSGMVEAFTDA
                     HGQFTFENFTLMFHSELFDKAVMNTAIFAAVSVVLQYFIALFLAVLLNRKFKGSKFLT
                     FIAMIPMAITPTAVAILWKTGLVKEGWINTILLALHLVKEPIVFLNYEGLAAVFLIIL
                     IDTWTVTPSIMIILLAGLQGMQKELREAAYTFGANKWQIFKDITLPILKPSIITSVIL
                     RLISAIQVWAIAVMVLGYGKAPFLVERIAFYVDVIPGVATSQKIAFSLSFTTTIIVFL
                     VTVFYLNATKQKNKLGGER"
     gene            complement(1678..2919)
                     /locus_tag="VF531_10770"
     CDS             complement(1678..2919)
                     /locus_tag="VF531_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714492.1"
                     /translation="MKKWLFSVLAIVCIASFAVTGYAKNEVLKIACAGAFQPHEKEYI
                     IGTFMKGFESKYKVQVEVDFISQADGIKKIQAEQESKNIVSDVLFVDTANMPPYVNGG
                     WVEDITKTVNGTRSTLTNMFDNTTTKDGKRYFVPISFDVYITIANKKALKYLPKGLKE
                     KNVVNGLTWEQYAVWAKAIAVGEGVGKTMLPANMTGSQLLYPMGGLGMAYGAKFPEFN
                     SDGFKKAMNIIADMASGNAFYPEQAQYSAPTDPLKSGAVWLTFAHMGPIGVAYSASPN
                     EYIIGAAPKGPRGAGSTAGAWCYGIQKGTKNRKLAEKFIAYVLNPRVNYEYCLEMGGV
                     LSPIKQVGKMMDSSDVIMIAGNKMLDNTIISGVPSSQYTDWNAVKLLYGELFNKILKD
                     KAVPDDQYFSDLQARLEKLQK"
     gene            complement(3215..4609)
                     /locus_tag="VF531_10775"
                     /pseudo
     CDS             complement(3215..4609)
                     /locus_tag="VF531_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
BASE COUNT         1324 a         1098 c          938 g         1300 t
ORIGIN      
        1 gataccgaca aaaatacttg atgtaatcca tgccgtaagc gctacattgg gaattgaata
       61 tagtaaagcc aaacccaaca aggtgtcaaa taaaccaaac ttaatgagta aaatggccat
      121 gggaatgctg attccaacca ccggaaacat tctcacaatc agcagcgtga tattgatggt
      181 ctctttaaat ttgaatgaat atcgggccat ggcatatcca gccatacttc cgataatcaa
      241 ggaaatcagg atggtgtata agcaaactaa aatgctgttt ttcagcgccg gtaatgagtt
      301 attgacaatc gcaaaaaact ggttgaaatt atagaaagta tttttcacat atgtaaactc
      361 catcaggccc tttttccttg ttttgccgaa gtcggccagc aatttttcgc ccggaacggt
      421 caccgaatat tgtctcttaa aatggttttc cagcttaata gggtttctag tcgtaatgat
      481 cgattcgtag ccttcccctt tgttatagaa tatttcgtat tcaccgtctg ctgccggagc
      541 gacctttacc gtgaccgtaa acttaggaat gattgacttt ggaaacttcg acatttcgac
      601 atcggatgaa aaagcaacat tcaaaaagaa caatagtgga gtaaccacag cgattaccat
      661 tgttgcgaga ataatgctaa tgacagcttt ttgaataatg ttcagcctat ccaatcaccg
      721 ctcaccccct aatttgttct tttgtttggt tgcatttaga tagaatactg tcactagaaa
      781 cacaataatt gtagtggtaa aggatagtga aaaggcaatt ttttgactcg ttgccactcc
      841 gggtattaca tctacgtaaa aagcgattct ttccaccaaa aacggtgcct tgccgtagcc
      901 taaaaccata acggcaatgg cccacacttg gatggctgag attagtctca aaattaccga
      961 tgtaataatg gagggcttta atatcggcaa ggtgatgtcc ttgaatatct gccatttgtt
     1021 ggctccgaag gtatatgccg cttcccgtaa ctctttttgc attccctgta aacccgcgag
     1081 taggatgatc atgatcgaag gagtcacggt ccaggtgtca atcaatatga tcaggaaaac
     1141 cgctgccaat ccctcatagt ttaggaaaac gatcggttcc ttcaccaaat gaagggcgag
     1201 caaaattgta ttaatccaac cttccttgac caagcctgtc ttccaaagga tcgccaccgc
     1261 ggtgggagta atggccatcg gtatcatggc aataaaagta agaaatttgg agcctttgaa
     1321 tttacggttt aatagaaccg cgagaaaaag agctataaaa tattgcagga ctacggagac
     1381 cgcggcaaag atcgcggtat tcatcacggc tttgtcaaat aattccgagt gaaacatgag
     1441 cgtaaaattt tcgaaggtga attgaccatg agcgtcagta aaggcttcaa ccatccccga
     1501 aaaaacgggg gtaagccaga aaaccgcgta ataaatgaag gccggcagca ataagagata
     1561 tggtatggct tttttggaat ttgataattt gcgatttatc aaaatgattg ctcccttact
     1621 ttcgaataaa aaaagagagg gatgttttta ggcatccctc ttttttcagg taaagcccta
     1681 cttctgcagc ttttccaatc gggcctgcaa atcggagaag tattgatcat ccggaaccgc
     1741 tttatctttc aagatcttat tgaataattc gccataaaga agtttcacgg cattccaatc
     1801 ggtatattgg gaagaaggca ctccggaaat tatggtatta tccagcatct tgttaccggc
     1861 tatcataatg acatcactgg aatccatcat tttgccgact tgctttatag ggctcaacac
     1921 cccgcccatc tccaaacaat actcataatt aactcgggga ttcaaaacat aagcaataaa
     1981 tttttcggct aatttccgat ttttggtgcc tttttggatc ccataacacc aggcgcccgc
     2041 ggttgaaccg gctccccggg gtcctttcgg agcagcaccg ataatatatt cgttgggaga
     2101 tgcgctgtag gccaccccga tgggtcccat atgcgcaaat gtcaaccaaa ccgctccact
     2161 cttcagggga tcggtcgggg ccgaatattg agcctgttcc ggataaaacg cgttccctga
     2221 ggccatgtca gcaataatat tcatcgcttt tttaaaacca tcggaattaa actcagggaa
     2281 cttggcgcca taagccatcc ctaatccacc catcgggtat agcaactggg aaccggtcat
     2341 attggccggc aacatggttt tcccaacgcc ttcaccgacc gctatcgctt tagcccaaac
     2401 cgcatattgc tcccaagtaa gtccgtttac gacattcttc tccttgagtc ccttaggtaa
     2461 gtattttaaa gcttttttat tggcaatggt gatataaaca tcgaaactaa tcgggacgaa
     2521 ataacgcttg ccatccttgg tcgtcgtgtt gtcgaacata ttggtaaggg tggagcgggt
     2581 cccgttaact gttttcgtaa tatcttccac ccagccaccg tttacatagg gcggcatatt
     2641 tgcggtatct acaaacagga catcggaaac aatatttttg ctttcttgct cggcttggat
     2701 cttcttgatt ccatccgctt gagaaatgaa atccacttca acttggactt tatacttaga
     2761 ttcgaagcct ttcataaaag tgccgattat gtactctttt tcatgaggtt gaaacgcacc
     2821 ggcacaagcg atctttaaaa cctcattctt tgcataaccg gttaccgcaa aactcgcgat
     2881 acatacgatt gctaaaaccg agaataacca ttttttcatt ttgtccctcc tctttatttt
     2941 ccctgcctag actcacaccc agcaaatgtc aaactggcaa tatgtgattt aagattatta
     3001 taataccaat aaaacgtttt tacaatacaa ccggcaacaa tattttattc attttgtaac
     3061 atttcataaa ccctttattt taaatggttt atgccgttaa ataagcaatc aataaacgaa
     3121 tatttcatta tgtatattta aaaaaccgtt tttttgataa tgccggcaac cagccaactc
     3181 tatcaattga taatgctgta cccaaataaa ggctttaggc attggcttca aacctccaac
     3241 atgtactcgc gaaatcaccg tgtaaattag gtaccgttat cccggcatac atcaattcgt
     3301 caccgttata gattcgtccc gttcccatga attggtaatt tctggactca tcgaggcctc
     3361 ttaacttgag gagggagacg agcggcggat tgggctttgc cagtatcctg aaataaaata
     3421 ccagcgcctg ccttacatct tcagatacaa acatccaagc cgcctcatta tcctcaaaag
     3481 gactcttcag cctgtaaaaa tccccgaatt ggaccaattc ccggacctct ttgtaatcgg
     3541 cgatctgttt tttcaccagt tccttctctg cggatgttaa tccggataga tccagttcat
     3601 atccgaggtt tccggacata gccaagtgcc cccggatttc aagattggta attctcccta
     3661 tttgatggtt gggtgccgcg gatacgtgag ctcccatcgt tactacaggg taaaccaggc
     3721 tggtgccgta ctgaatatat aatctttcaa cggcatccgt atcatcgctg gtccaagtct
     3781 ggggcatata gtacaatatt cccggatcaa atctgccccc gccgccggaa cagctttcaa
     3841 acagtatttg ggggaaaatg gaggtgatgg cctccattat tttatacaac cccagcatat
     3901 accgatgggc agtttcgcgt tgtctctccc gcggcagaaa agccgaaccg gattccgtca
     3961 tattccgatt catgtcccat tttacataac ggatcggagc gctgtttaaa atctcactga
     4021 cggctttcac gatataatcg caaacctcat cattggtcaa atcaaggatc aactgatgtc
     4081 tggactgggt ccggttccga tcgggaacat gaatgcacca atccgggtgg ttccggtata
     4141 actcgctatc gggggatatc atctccggct caaaccacaa accgaactcc agtcccagac
     4201 ggttcattct ttgcgcaaga tctgccaacc cctcgggaag cttctctcgg ttaacatacc
     4261 aatctcccag ggaacaggta tcactgtttc tcctgccaaa ccagccatca tccagaacaa
     4321 acagctcgat tcccaattcc ttggcgatct ttgccatgtc cagtaacttt tctggggtaa
     4381 aatcaaagca tgtaccctcc caattattga tcaggatggg cctgtttttt atcttaaagc
     4441 ttccgcggca caagttgttc cgataaagcc ggtgaaaggt tcgggacata tcgccgaggc
     4501 catgacagga atagaccatg ataagctccg gcgtttgaaa acttcccccg ggttctaaaa
     4561 gccaacaaaa atcgaaaggg tttatcccca tcgagaatct ggttgtttta gtcgatcaat
     4621 ttaaaacaac cagattctcg atggggataa accctttcga
//
LOCUS       DASVMB010000380         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_271231,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000380 DASVMB010000000
VERSION     DASVMB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271231"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..606)
                     /locus_tag="VF531_10780"
     CDS             complement(166..606)
                     /locus_tag="VF531_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX7714493.1"
                     /translation="MVEPNVIVRILTLLDEYLRDLEEIRQTKKLQDFLTDKVTRRYSE
                     RTLQIAMEACLDIAGHIISYEGLREPVDNKDCFQVLYETGIVPNDLSERLKKMAQFRN
                     VVVHDYLRINPEIIFSIVQRNLPDIASFAAIIKRKYIDSKDDRK"
     gene            complement(612..1028)
                     /locus_tag="VF531_10785"
     CDS             complement(612..1028)
                     /locus_tag="VF531_10785"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7714494.1"
                     /translation="MINRQILQKLTSYFLERGELSAAYLFGSHVKGKNRPHSDLDLAV
                     LFDPKLDVFQRFDNKLQIANDLEESLNVKIDLVDLESADLFFIHQIMLDKLLIYEKDR
                     ERRVEFEVKARRDFFDRQHFYDLYYREALKRIEGRS"
     gene            1204..2529
                     /locus_tag="VF531_10790"
     CDS             1204..2529
                     /locus_tag="VF531_10790"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEX7714495.1"
                     /translation="MCDTMVALGNVTQNGKALFAKSSDRQPNEPHIMVRVPGRKRTQD
                     SKQSIQTTYIQIPEPDETYEVFLLKPSWIWGCEMGFNEFGLNIGNEAVFTKEKYGPPA
                     LLGMDLARLALERCRTSEEAVGTITKLLEEYGQGGNCGFAKPFTYHNSFLIADPRSAW
                     VLETAGPYWAAKKVKDVYAISNCLTLGSDYDKCHPDLVKRAVDKGWCRGEKDFSFRDC
                     YSDKLFTHFSGAWVRCNTSQASLERESGSITATTLKTILRSHEECFAGREFKNGSVRS
                     ICMHAGGLIGDHTTGSYIGELGEKRSTYWVTGASTPCLAVFKPVWPVDDVPFFTEEDQ
                     TAAVDYWKERERIHRLALAGLIDRDEYRTERDRLEISFEQLPQVTNPEAATDEELARV
                     SQTAFARENQLIAAYLERAQRSGRNPKIQGGWFFNHYWQGQNRKLPLEF"
     gene            complement(2537..3301)
                     /locus_tag="VF531_10795"
     CDS             complement(2537..3301)
                     /locus_tag="VF531_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7714496.1"
                     /translation="MKRQSMRKTLILISFFLFPVTIYYLSPALPIEGAFQGIASGSLV
                     LFALLFLSSLICGRAFCGWVCPAAGLQEACFGIQNKRVVRGYWVKYLIWGCWMAVLIL
                     AVISAKGIHALDPFFATDHGISIAEPSNYIIYYSVIALIFIPALGIGKRSFCHSICWM
                     APFMVLGMKIRNWIQWPALHLETNPERCSHCQRCTRECPMSLEVETMAKDGTIANSEC
                     ILCGNCVDTCPKMAIHYAWMKKRSLPAATTATRNTH"
     gene            complement(3317..3931)
                     /locus_tag="VF531_10800"
     CDS             complement(3317..3931)
                     /locus_tag="VF531_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7714497.1"
                     /translation="MPQDNRTIYIILGLLSHEPMTGYEIKRRVTTTISFFWEVGYGQI
                     YPALRQLQKDGLVTMKPEARENRPRKVYTITESGRTELKNWLHIPAIRESERHEILLK
                     LFFGSQIPVQANLQNIASFRKRSAAVLETLTAFEENLRPITSNPDHLYYLLTVRFGEY
                     LYKAYLDWVDEATATLQKFTSGEGHPPSGAEDAESLGLNTSQRK"
     gene            4128..5357
                     /locus_tag="VF531_10805"
     CDS             4128..5357
                     /locus_tag="VF531_10805"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7714498.1"
                     /translation="MRIGEFAEKHGVTIDTVRHYMDEQLLMPEKSGGQYEFGDACCKD
                     LEEILWLKKAGFTLAEIKKALSIERLGKLSSSYDRQCYIKLLQGKKGDLAEEIVEREE
                     SIKKIEGRMAEFKTDMGTGSNKIGVPLEFASLLACPQCGKSMVINGAIINSMIIDGQI
                     TCRCGFALSIVNGIITNAPEEVKSKAYYFSDADSYEEYIAKTSIKFINYVHRSVEWII
                     AKLRLDLVKQPTILELGTGWGSLLKHAYGMFTEDVLYIATDNDIEALTLTKWILEKEA
                     SRTKFVFIHGNIEDIPLKKETVDLVLDFWGTSCHNFKKPGYLINYIKDRLKPGGRWLA
                     GHLYFKEDSSSLKRLAKECRLYLYLDNIIGSYQNNGFTLLDSKDMGYIDEGGEYEPFL
                     VKGDKAFEWVYYGIKYK"
     gene            5523..6767
                     /locus_tag="VF531_10810"
     CDS             5523..6767
                     /locus_tag="VF531_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7714499.1"
                     /translation="MFRPLKQSSFLKLYIAQVFSDLGNWLDFIALNVIIAYRWELGPE
                     MLAAETVAFGLPWIVFGPVFAVYTDRFSKRIAMGLSHVLRILTAIGFIFVPNILLLLA
                     LILIKETISALYDPLQQGAIQMLVPRELLPEASSLSQLSSNTAKIAAPALGGLLIALN
                     GPKTVFLIEAAGFLASAILVFRLYALDTVIQTKGVKRESLNGFRKEFLSGLAFIRANK
                     TLKIAVYFMSARIFLIFLYDSMLVIWVKGINLSESAFAWLISAIGLGSVLGSLIFGHW
                     KGWVRKPLVVMGLASAFSGGMVLLIALIGYGFRVADVYLLSLMFLVFGMVGAAVPTVY
                     GYLVQSQTVPDLLGRVVSTANALQNCFMLSAPLIGAILAKISGINAVFAISGVFTCLL
                     GIVILIQGKRYSGAPLISPDIS"
     gene            complement(6875..7756)
                     /locus_tag="VF531_10815"
     CDS             complement(6875..7756)
                     /locus_tag="VF531_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX7714500.1"
                     /translation="MPWINLFLSIIFWSALSVVIPSGASQSALASAPATTGHPLGKPA
                     QSLLIWENDEEFLRVCEENQAQVRMAAFQTTLPDPLPGEEYNVGLAAQLLAGTVVPPG
                     GVFSMNGTIGRRTRERGFREGPAYSGSQIIRVTGGGVCKISTTLYNVAILADMAVVER
                     KPHGMLVPYVPPGQDATVSYGTVDFKFRNTYKFPVVIWAEKVGNTLYMAFYGRIRPPR
                     IIWDHQILNRQPIPTLYLPNPRLPRGTKKVIQAGAEGITVKNRLIIHYPSGETVLREL
                     GTDYYRPMARIVETGPG"
BASE COUNT         2004 a         1784 c         2053 g         2079 t
ORIGIN      
        1 gatttctacg ccaaacaggc ataccgactt ccgttatggg ttgacggaaa atacaaataa
       61 gcacatttca ggagtatact tactaacata ggcgactcat cattgattcc taagctactt
      121 ttaccgtaaa ccgtcatggg caaatgtaga tgcaggcagc catttctatt ttctgtcatc
      181 tttggaatca atatactttc gtttaataat tgccgcgaag gatgctatat cgggaagatt
      241 tcgttgtaca atcgagaaga taatctccgg attgattctc aaataatcat gaactaccac
      301 gttacggaat tgggccatct tttttaaccg ctcagagaga tcattcggaa cgatcccggt
      361 ttcgtacaat acctggaaac agtccttgtt atccaccggt tcccggaggc cttcgtatga
      421 tatgatatga ccagcgatat cgagacaagc ttccatggcg atctgcaggg tccgttcgga
      481 atatctgcgg gttactttat cggttaaaaa atcttgcagt ttcttggttt gacgtatctc
      541 ttccaaatcc cggaggtatt catcgaggag tgtaaggatt cttacgatca cgtttggttc
      601 aaccatcgaa atcatgacct cccttctatc cttttcaagg cctcccggta ataaaggtcg
      661 taaaaatgtt gtctgtcgaa gaaatcacgg cgcgctttaa cttcgaattc tacccggcgt
      721 tcccggtctt tctcataaat cagcagttta tccagcatta tttgatggat gaaaaataaa
      781 tcagcgctct ctagatccac tagatcgatc tttacgttta aagattcttc aaggtcgttt
      841 gcaatttgga gtttattgtc gaacctctga aatacatcga gtttgggatc gaataaaacc
      901 gccagatcga gatcgctgtg ggggcggttt ttacctttca cgtgggagcc gaacagatat
      961 gcagcggata gctcgcctcg ttccaagaaa tatgaggtta gtttttgaag gatttgccta
     1021 ttgatcatag gtcaatctcc cattcccatt tttcaaatcg taataaccat attataccac
     1081 agcccccaaa cctttaacat aaatggatta cccggaccgc atagcttaac tctgccttgg
     1141 caggaaaaca gccagtgagc ccgaagatat agataaaagt ttaaaagtaa gcgggtgtta
     1201 gtgatgtgtg atacaatggt agcccttgga aatgtaacgc aaaacggtaa ggcgttattc
     1261 gccaaaagca gcgataggca gcctaatgag ccgcatatca tggtccgggt tcccggcaga
     1321 aaacggaccc aagattcaaa acaatctatc caaactactt atattcaaat cccggagccg
     1381 gatgaaacct acgaggtgtt cttgctaaag ccctcctgga tctggggttg cgagatgggc
     1441 ttcaatgagt tcggcctgaa tatcggaaat gaggcggtat tcaccaaaga aaagtacggg
     1501 cctccggctt tgctcggcat ggatctggcc cggttggcgc tagagcggtg ccggaccagc
     1561 gaggaagcgg ttgggaccat cactaaactc cttgaggaat acggccaggg tgggaattgt
     1621 ggttttgcca agccttttac ctatcataat tcgtttttaa tcgcagatcc ccggtcggcc
     1681 tgggtgcttg aaacagcggg accttactgg gccgcaaaaa aggtcaaaga cgtctatgcc
     1741 atctccaact gtttgaccct cgggagcgac tatgacaagt gtcatcccga cctggtgaag
     1801 cgggctgtcg ataagggctg gtgccgcgga gagaaggatt tcagtttccg cgattgttac
     1861 agcgataaac tattcaccca tttcagcggc gcctgggtac gttgcaacac gagtcaggca
     1921 agtctggaac gagaaagcgg ctctatcacc gcaaccacgc ttaagaccat cctgcgcagt
     1981 cacgaggaat gctttgcagg cagagagttt aagaacggtt cggtacggag catttgcatg
     2041 cacgctggag gcctgatcgg tgatcatact accggcagtt acatcggaga gttgggggag
     2101 aaacgctcta cttattgggt gaccggcgct tccaccccgt gcctggcggt attcaaaccg
     2161 gtttggccgg tagacgacgt gccgttcttc acagaggagg atcagacggc cgcagtggac
     2221 tattggaaag agcgggaacg gatccatcgg ctcgccttag cgggattgat cgaccgggat
     2281 gaataccgta ccgaacgaga taggttggaa ataagctttg aacagttgcc ccaggtaacc
     2341 aatccggaag cggcgaccga tgaggagctc gcccgggttt cccaaactgc tttcgcccgg
     2401 gagaaccaat tgatcgccgc ctatctggaa agggcccaac gttccggaag aaatcccaag
     2461 atccaagggg gatggttttt taatcattac tggcaagggc agaaccgcaa gctacccttg
     2521 gagttttagt tcgatgttaa tgcgtattcc gtgttgcggt cgttgctgcg ggaagcgacc
     2581 gctttttcat ccaggcgtaa tggatcgcca ttttcggaca ggtatccaca cagttgccgc
     2641 ataaaataca ttcggaattg gcaatggttc cgtccttagc catcgtttcg acctctaagc
     2701 tcatagggca ttctctggtg cagcgctggc aatggctgca gcgctccggg ttcgtttcaa
     2761 ggtgcaaggc cggccattgg atccagtttc gtattttcat accgaggacc ataaacggcg
     2821 ccatccagca gatggagtgg cagaaggaac gtttaccgat ccccagggcg gggatgaaga
     2881 tcaaagcgat caccgagtaa tagatgatat aattgctggg ttcagcgatg gaaatcccat
     2941 gatcggtcgc aaaaaagggg tccagggcat gaatcccctt ggcggatatc actgccagga
     3001 tgagtaccgc catccagcaa ccccagatca ggtatttaac ccagtatccc cgaaccaccc
     3061 gtttattttg aatcccgaaa cacgcttcct gtaatcccgc agcaggacag acccaaccgc
     3121 aaaaggccct accgcaaatg agcgaactca agaaaagcaa tgcgaataag accagactac
     3181 cgctggcgat tccttgaaag gccccttcaa tgggaagcgc tggagataaa tagtagatag
     3241 tcaccgggaa tagaaagaat gagatcagaa ttagggtctt ccgcattgat tgccgcttca
     3301 tggttcctct cctctgttat tttctttgtg atgtattcaa cccaagagat tcagcgtctt
     3361 cagctccgga tgggggatga ccctccccgg aggtaaactt ctgcagcgtc gccgtggctt
     3421 catcaaccca atccaaatat gctttataga gatactcacc gaaacgcacg gtcagcaagt
     3481 aatagagatg atcgggattt gacgtaatgg ggcgtagatt ctcctcgaag gcggtaagcg
     3541 tctcaagaac ggcggcagat cgcttgcgaa acgaggcgat attttggaga ttggcctgga
     3601 ccggaatttg gctgccgaag aagagtttca agagaatttc gtgccgctcc gattcccgga
     3661 tggctggaat atgaagccag ttttttagtt ccgtgcgccc ggattcggtg atggtataca
     3721 cttttcgggg acggttctcc cgcgcctccg gcttcatggt gaccaggccg tctttttgaa
     3781 gttgtcgcaa tgccggatag atttggccgt aaccgacctc ccaaaagaaa ctgatggttg
     3841 tggtcactct ccgcttaatc tcataaccag tcatcggttc gtggctgagc aggcctaaga
     3901 tgatataaat ggtgcgattg tcttgcggca taggtggaac tccattcaaa aggattattt
     3961 ttaatatatc attttgatat atatatgtca atgagcattc gttcgattaa aaacgacgga
     4021 gtaaaactta acttgacgat tgacgataaa taggattgat ttgttttagt tttttgatgt
     4081 aagcttaaaa agattcatcg gaaataaacc aaaatgggtg tgattgtatg cgtatcgggg
     4141 aatttgcgga aaaacacggg gttaccattg atacggtaag gcattatatg gatgaacagc
     4201 tattgatgcc tgaaaaatcg ggtgggcagt atgaatttgg cgatgcctgt tgcaaggatc
     4261 tggaggagat tctgtggttg aaaaaagccg gttttaccct ggccgagatt aaaaaggcgc
     4321 tctccatcga acgccttggc aagctcagca gcagttacga caggcaatgt tatattaagt
     4381 tgctccaggg taaaaagggc gatttggccg aagaaatcgt tgaacgtgag gaaagcataa
     4441 aaaagattga gggaagaatg gccgaattta aaaccgatat ggggacgggt tccaacaaaa
     4501 tcggggtgcc gcttgaattt gcttcccttt tagcttgtcc tcaatgtggc aaaagcatgg
     4561 tcataaatgg cgccatcatc aatagtatga taatcgacgg ccagattaca tgccgttgcg
     4621 ggtttgcctt gagtatcgtc aacgggatca ttacgaatgc gccggaagag gttaaaagca
     4681 aggcatatta cttttccgat gcggatagtt atgaagaata tattgccaaa acatcaatca
     4741 agtttattaa ttacgtacat agatcggtag aatggatcat cgccaaactg cggctggatt
     4801 tggtgaaaca acctaccata ttggagcttg gcacgggttg gggttcgtta ttgaagcatg
     4861 cctacggtat gtttacagag gatgttttat atattgccac agataacgat atcgaggcgc
     4921 tgacgctcac caaatggatt ttggaaaaag aagcctctcg gaccaaattt gtttttatcc
     4981 atggaaacat agaggatatt ccgcttaaaa aagaaacagt ggatttggtt ctggattttt
     5041 ggggtacgtc atgccataat tttaaaaaac cgggttactt gataaattat attaaggatc
     5101 gcctgaaacc cggaggaaga tggctggcgg gacatcttta ttttaaggaa gattcaagtt
     5161 cgctgaagcg attggccaag gaatgccgcc tttatctata tcttgataat attatcggtt
     5221 cttatcagaa taacgggttt actctactgg attccaagga tatggggtat attgacgagg
     5281 gcggagagta tgaacccttc ctggttaaag gcgataaggc tttcgaatgg gtttactacg
     5341 ggattaaata caagtaatac taagttgctt tttgaaacaa cttagtatta aacatggggt
     5401 taaccccatg ttttttgttt tttaaccgcc aaaaaggctt tgcgagctac ttgatgtcgg
     5461 cttaacccct caggttataa tcggaaccga gataaaaaag ccgctggact tgaggtggtt
     5521 ggatgttcag gccattaaaa caaagttcct ttttaaagct ctatattgca caggtatttt
     5581 cggatctggg caattggttg gatttcattg ccctaaatgt gatcatcgct taccgttggg
     5641 agttaggccc ggaaatgttg gcggcggaga ctgtcgcctt tggcttgccg tggattgtct
     5701 ttgggccggt atttgctgtt tatacagaca gattttccaa aaggattgcc atgggattaa
     5761 gccatgtttt gcgcatttta accgcgattg gcttcatttt tgtccccaat atcctccttt
     5821 tgctggctct cattttaata aaggagacaa tcagcgcctt gtatgatccc ttacagcaag
     5881 gcgccatcca aatgctcgtc ccgcgggaat tgttgcccga agccagctcc ttgagtcagc
     5941 tttccagcaa tacggccaag atcgcggctc cggctttggg ggggttactc atcgcgctga
     6001 acgggccgaa gaccgtattc ctaatcgagg ctgccggttt tttagcctca gcaatcttgg
     6061 tttttcggct ttatgctctc gatacggtta ttcaaacaaa gggtgtcaag agggagagtt
     6121 taaacggttt caggaaggaa tttctaagcg gactggcttt tatcagagcg aacaaaaccc
     6181 tcaagattgc ggtttatttc atgtcggccc ggatattcct tatttttctt tacgacagca
     6241 tgctggtcat atgggtcaag ggtatcaacc tgagcgaatc ggcgttcgcc tggctgatta
     6301 gcgcgatcgg gctggggagt gtacttggtt cgctaatctt cgggcactgg aaaggatggg
     6361 tacgcaaacc cctagtagtg atgggcctgg cttccgcttt ctccggcgga atggtgctgc
     6421 tcatagcctt gattgggtat gggttcaggg tggcggatgt ttatctgttg agtctgatgt
     6481 ttttggtatt tgggatggtg ggagccgcgg tgccgacggt ttacggttat ctcgtccagt
     6541 cgcagactgt tccggatcta ttgggccggg tggtaagcac agccaatgcc ctccaaaact
     6601 gttttatgct gtcggcaccg ttgatcgggg ctatacttgc caaaatatcg gggataaatg
     6661 ccgtattcgc aatatccggt gttttcacct gtttactggg gatagttatt ttaatccagg
     6721 gcaaacggta ttccggtgct cccctgatat ctccggatat tagttaagcg tggtccatgg
     6781 ttatccgcta tccggcaaac caattcgcct cagccttatt tgaaaggatt ctttcacagc
     6841 taaaactgcc actttgccgg gagcccatcc gggctcatcc cggaccggtc tccacgatcc
     6901 gggccatcgg ccggtaatag tcggtaccca gttccctcag gaccgtctcg ccgctcggat
     6961 aatgaatgat caagcggttt ttgacggtga tcccttctgc gccggcctgg attacctttt
     7021 tggttccccg gggcagacgg gggttgggca agtagagcgt ggggatcggt tgccggttaa
     7081 gaatctggtg gtcccaaatg atccggggag gtctgattct gccgtagaag gccatgtata
     7141 gcgtgttccc gactttttcc gcccagatga ccacgggaaa cttgtaggtg ttccgaaact
     7201 tgaaatccac cgtaccatag gaaacggtgg catcctggcc cggcggtaca tagggaacca
     7261 gcattccgtg gggtttacgc tcaacgacgg ccatgtcggc taggatcgcc acattgtaga
     7321 gggtggtgga aatcttacat actccaccgc cagtcacccg gatgatttga ctgccggaat
     7381 aggcaggtcc ctcccggaag ccgcgttcac gggttcgtcg cccaatggtt ccgttcatgg
     7441 agaagacacc cccgggtggg acaacggtcc ccgccaataa ttgagcggct aggccaacgt
     7501 tatattcctc gccgggaagg ggatccggca gggttgtctg gaatgccgcc atgcggactt
     7561 gagcttggtt ctcctcgcaa accctcagaa attcctcgtc gttttcccag atcaacaggg
     7621 actgggcggg tttgcccaat ggatgtccgg tggttgccgg agcggaggca agcgcggatt
     7681 gggaggcacc ggatggtatg accaccgata aggccgacca gaatatgatc gataaaaaca
     7741 ggtttatcca cggcattttt aggttgaacc tcttcgaaga tttttagcta agtattaccg
     7801 gcgacgctga tttgtatgtg aataaaccat catggaacgg ttggcgaggc acctttaccg
     7861 ctatcccttg atcaatacag tcaacagaaa atccgctaaa ttttttcatg aagtctgacg
//
LOCUS       DASVMB010000381         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_193701,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000381 DASVMB010000000
VERSION     DASVMB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193701"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1646)
                     /locus_tag="VF531_10820"
     CDS             complement(90..1646)
                     /locus_tag="VF531_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7714501.1"
                     /translation="MPQKSYYIKNEQETNRIVIKILLWILLVFPVILLLSRSVLGFFP
                     GDYTVYLWPSICGTVFNLTPYILLRCNAKSSIVKYAAIIAATFTVGVINANPEMYISI
                     ILIMPIALSLLYFDRKLAFFSFAITLAMIAISQYLFDSALYTRFGIAGVESKPLLIEW
                     IWDVAIYTIEVIVLSLIFSILTRRTRALFESLANSEEQIRLLDKLQDVMKESTRASGI
                     LATSVKQLTVITEDTSRANQLISQAATTAVQSGVKNLEYMDTTHNTIADVSQLFSTML
                     AHAQGLSEISKATYQAATESELVISQAVKKMEEIEAFTLQNKELMNRLGERSQQIGKV
                     IGIIARITRQTNLLALNAAIESSRAGEHGRGFTVVAQQIKELAEQSAKAAGEISQLIQ
                     LEQQDALSAVESIDQDALNVKSGLELVQTAGTSFEKLKAMQEESDNKAQEIAEFIRRS
                     NEYGRTINEIISNIKESTQKTNADVAAIAGAVEAQLDSMRQVTASVVVVDQIADSLYN
                     LSNGIRKFKN"
     gene            complement(1841..>2595)
                     /locus_tag="VF531_10825"
     CDS             complement(1841..>2595)
                     /locus_tag="VF531_10825"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7714502.1"
                     /translation="KLKELRLPALFVKSGNEDGNSQTLVSDAARSDLTQALYKLDSNF
                     RAGKAAGFSSCRIPLHNLVDEIVFNQRMSLDAADIRFQNDQIYGHMVNVCIVAVKIGL
                     EMGYNQLKLAELAYGVIFHDIGMVKIPVDILNRIGGLTNEETRLIQTHPKTGYELLRQ
                     SPDVSAASAHVAYEHHERFNGSGYPRGLVGEAIHEYARITAVADVYDAMCTEKIYRPA
                     KTVPEIVDYLQNESGNAFDPNVVEIFTKVIAH"
BASE COUNT          681 a          595 c          603 g          716 t
ORIGIN      
        1 taatgagcag atcgagatgg cgatccaaat gtaaaaagag ttccaaaaga ttatcgaaaa
       61 attccatgag cgctcgcttt ctttgtaatt cagtttttaa acttacgaat gccgttactc
      121 agattataaa gactgtccgc aatctgatct actacgacga ccgaggcggt tacttgacgc
      181 atggagtcca gctgcgcttc gactgccccg gctatggcgg ctacgtcggc gttggttttt
      241 tgggtcgact ccttgatatt ggagataatc tcgttgatag tacggccata ttcattggaa
      301 cgccgaatga attcggctat ttcctgggcc ttattatcgg actcctcttg catcgccttc
      361 agtttttcaa atgaggttcc tgcggtttga accagctcaa gcccggattt aacattcagg
      421 gcatcttgat cgatagattc caccgcactg agggcatctt gctgctccaa ttgaatcaac
      481 tgagatatct ctccggccgc tttggcggat tgttcggcaa gttccttaat ctgttgagca
      541 accactgtga aaccccggcc gtgttctccg gcacgggaag attctattgc cgcatttagg
      601 gccagtaagt tggtctgccg agtgatccgg gcgatgatcc cgatgacctt gccgatctgc
      661 tgcgaacgtt caccgaggcg gttcattagt tctttgtttt gaagtgtgaa agcctcaatc
      721 tcttccatct tcttgactgc ttgactaatg accagttcgc tttcggttgc cgcttgatac
      781 gtggccttag agatttcaga cagtccctgg gcatgtgcga gcatggtact gaataattga
      841 gagacatcgg caatggtgtt gtgggtggta tccatatatt caagattctt tacaccgctt
      901 tgcaccgcgg ttgtcgccgc ctgggaaatg agttgatttg cccgggacgt atcttcggtt
      961 atcactgtca gttgctttac ggaagtggct aaaatccctg aagcacgggt cgactccttc
     1021 atgacatcct gtaatttgtc gagcagtctt atctgttcct cggaattggc caaactttcg
     1081 aagagtgccc gggtacggcg agtcaatatc gaaaagatta gggaaagtac gattacttca
     1141 atggtataaa tggccacatc ccaaatccat tcaatcaaaa gtggtttact ctcgaccccg
     1201 gcgataccga aacgggtata taaagcgctg tcaaaaagat actgtgagat ggcgatcatg
     1261 gccagggtta tggcaaagga gaaaaatgcg agtttacgat cgaaatataa gagacttaag
     1321 gcaatcggca ttatcaagat gatgctgata tacatctccg gattggcatt gatgactccc
     1381 accgtaaaag tggcggcgat gatagctgca tatttgacga tggagctttt tgcattacat
     1441 cgtaagagga tataaggggt gagattaaaa acagtcccac atatgctcgg ccataaatat
     1501 acagtataat ccccgggaaa gaaacccaaa acggaacgtg acaataataa aatgaccgga
     1561 aaaaccaaaa gtatccagag tagaatcttg atgacaatcc ggttcgtttc ttgttcattt
     1621 ttgatatagt aagatttttg cggcattccc tctcccccat gtgttcgcca gtactagccg
     1681 tgcgcgtatc tgtgcattat tgagcccggt acgatcagga ttacgtatac gcgccaagca
     1741 ttgatgatat aaaccgattc gtggaaatac cgaaaattcc tgctttttac ctggtaaaaa
     1801 ccaaaaaata aagacctgca gtcgatgcag gtctttaaga ttaatgggca atcaccttag
     1861 tgaagatttc cactacattt ggatcaaaag cgtttccact ctcattctgt agataatcta
     1921 ctatttcagg aacggtcttc gcgggacggt atattttttc ggtgcacatg gcgtcataga
     1981 catcagcgac cgctgtaatc ctagcgtatt catgtattgc ttccccaacc aatcctcttg
     2041 ggtatcctga tccgttaaag cgttcatggt gttcataagc cacatgtgcg gaagccgcgg
     2101 aaacatcggg actttgacgg agcaattcat aaccggtttt gggatgggtc tgaataagcc
     2161 tggtttcttc gttcgtcaga ccaccgatcc tgtttagaat atcgacggga atcttaacca
     2221 tcccgatgtc atggaaaata accccatagg ccagttcagc cagttttagc tggttatatc
     2281 ccatttccaa gccgatcttc accgccacaa tgcaaacatt aaccatgtga ccatatatct
     2341 gatcgttctg aaaacgaata tcagccgcat caagactcat ccgttgatta aaaacaatct
     2401 cgtccaccag attatgcagc ggtatcctac aggaggaaaa acctgccgct tttcccgcgc
     2461 ggaagttgga atccagttta tataaggcct gggtgagatc ggaccgggcg gcgtcggaaa
     2521 cgagtgtttg ggaattgccg tcttcattac cggatttaac aaacagtgcc ggtaaacgca
     2581 actctttcag cttgg
//
LOCUS       DASVMB010000382         9566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_122788,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000382 DASVMB010000000
VERSION     DASVMB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122788"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2943)
                     /locus_tag="VF531_10830"
     CDS             complement(<1..2943)
                     /locus_tag="VF531_10830"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7714503.1"
                     /translation="MEKGIIERPRLLQKMGDIQTRKATFLIAPAGYGKTVAMAQFIAV
                     MEQPVVWYQIDAYDNDPAIFLQYLTRGLQQYYPNFGEQFLLLLEQENIETRLRIILTK
                     FINSLIRVASEPIIFVLDDFHEITSPLVFRFIQEFLEYLPSHVHIMIAGRVAPLFTFT
                     RLQVADEILVIGAEDLSFIDLEVNEFLLKNAPHLSVESSAVLNSKVHGWPAAFRLAVG
                     AVSSTAASLELHETNMGIYDYFAKEVFEQQPEEIREFLIKTSVFETISVSDSDVLRGR
                     KDSAKILKWLEKQNLFLTPLAGTEKTYRYHQLFREFLLERLGPAKSQLQCEAGQIAEK
                     NGDLAKAIEYYIASGTASRIRAIIKIAGPQALQKGRWQTVERWLGFFSRKEKAADPKL
                     ALLQAQAEAFHGRIEEVSHWVNLAIAEFTILEDRTGLAESRLLKARILRYQGFYQQSL
                     VILENVTEILNDRDRLRFDLPLEKSLCLLMIGRFREAENLLKQALEIAENENALYTVA
                     YLLDGLGNTYYLQGVYSKALQAYRKRSEVLTGQNMPDYYMQNYNAAIYQEWGELEQAF
                     EYAKHSVEFKENYGLTDTLPSAYFQLGDIYLNRREPKTAEKYFRRAIDLTQNYSGEYF
                     FLILNKAYLGISLSLQGRLSEAQLITEAALEEARIPGGLILALCQELAAQIFWRYGER
                     QVAKEIVLEAAAVLEKMGFPRPLGHAYAFLTLSYTVEGDHMAAALYAKKTLEISAPRN
                     FYQSFLTSFDIFQPVLRFGLEQGIEVPFIQTILARLGKQAFPLLNSLRNHPDPKVRWR
                     TITPLSELSDGQALTALQALTEDPDTEVAETARGMLSRFASLRASENENTFEAFCFKT
                     LGPFQIFQKGVGVRVNWRTSKSRDLLALLVHQGKPVSRDVIIEELWPEHDLEKAQSLF
                     HTCLYYLRQILKKIGFPEIIYHNNTYSIKEGCYVDERRQFQDLITAGFRRENTAEATC
                     DFLKRAITL"
     gene            3351..3902
                     /locus_tag="VF531_10835"
     CDS             3351..3902
                     /locus_tag="VF531_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714504.1"
                     /translation="MFLKTFKKSFIFGLLLLVAILMMTGCGGTSNPGGSNPGDSDPGS
                     DPAGDGPSSALIGTWNSSGINPSIRVTLGECLGSINKPELNVVEYYYKGEVSCSVLKD
                     GKKTISTMPSLSDIDSICIQKSLDSEVLIIMAGLSDDSSGDLSVSGYIESNQLKAVVC
                     IDDPSGNTIYATPEGSYDIFTKN"
     gene            4246..5826
                     /locus_tag="VF531_10840"
     CDS             4246..5826
                     /locus_tag="VF531_10840"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7714505.1"
                     /translation="MNHWKEAAASREAATSREETPDALSEHYGTNLSRFRELLGTSAD
                     LVIREFTIDAEKVRQAAIIYLENLVAKNRIHEDILRPLTVNAATIGARNIKPNQITAF
                     LQSQLVTVSPVEQERTFSKAVDRIFSGDTILMIEGINEAFIFATRGWAQREVGKPESE
                     ETVRGPREGFVETLGTNVALLRRRIHHSDLMVEAHQVGQKTKTAINIVYLKGVVNEKI
                     VAEIRRRLARIQTDGILGSGYIEQLIGDAPISIFSTTSYSERPDAVGAKILEGRAAIL
                     IDGSPEVLTVPALFIESFQNPDDYNSHPFYATLIRWVRYIAFAVSIFAPAVYIALASY
                     NQELIPTPLFITMVAGAEVTPYPTFIEVVIIGLLFEIFREAGIRFPRPYGQGIIFAVA
                     FVLAQAPVTMGLVGEATVVVVALTAMASLVVPKQADASILIRLLLAILASVLGAYGIL
                     LGLLGVLAHLVSLRSFGVPYLAPIAPFNIRDFKQDVIQRTPLWAMLFRPRTIGWHDPK
                     RQKSELTPAPPDSSEEGQ"
     gene            complement(5982..7103)
                     /locus_tag="VF531_10845"
     CDS             complement(5982..7103)
                     /locus_tag="VF531_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX7714506.1"
                     /translation="MAPIPEAGIINTKQYTWMLFVIITSFTAIQIPGLVIFQAGRDAW
                     LSVIAAWFIDVMLALVYAYMGIRFPGENPVQYSSTILGKFLGKVAGLLFILFYLVVST
                     ILMRGLSMQIINILLPQTPNEVILLGAFMVTAYIVRKGIEVNARICEILGPLYLGSII
                     ISFLLSLPFSHLIRLKPQLDQGFYPFLSGTPLILSFIGICVSMGWYSAVCNRPENGFL
                     AKFSAVSMGLVMVNILVLICTGVFGAEQAGNMVNPGLQLTRLIHISTYFERMEVVWIV
                     IAIGAGIMTAVNALWIFSLGIAQIVGLNTYKPLVYPVALIAFLLSVTSFPNNTVYLNF
                     IFYVFPIIGILVESGLELFLFLTALILGKRGAGTLGNRS"
     gene            complement(7199..7360)
                     /locus_tag="VF531_10850"
     CDS             complement(7199..7360)
                     /locus_tag="VF531_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714507.1"
                     /translation="MLKLVIILAVIIGFFEGIPLAREKKWRELMVVGALIGIALLIDI
                     GKALELPTL"
     gene            complement(7367..8581)
                     /locus_tag="VF531_10855"
     CDS             complement(7367..8581)
                     /locus_tag="VF531_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7714508.1"
                     /translation="MLRLHLRVMFPIILLLFLSGCWDQSEIKEQAIVIGIGIDKIPGN
                     GAILLTAQVINPAASKNGNQGTSGSGTTGTTGGTSQATGSIVIETSTGRSFDDAIRNF
                     LKYSSRRVIFFHNRIIIFGKTLAESGIAGILDCMARDSQFRRTNWLLVAEANAREILQ
                     SKTGLGSYPAQEINQMMINLVKRAFFLPVTLNDFLTGMYSEGKTSLMPMVAVGAPGTD
                     PGQGGIQLERTAVFKNDRLIDVLTVEESQGLLWLADKQKGGSLVFPFQTGKGRKTISV
                     AIMNGSTKIYPRITPEGILMDIFCTGTGTLRESEGLKNNPRTIQQLERRVESILKNRL
                     ERTIQKAQQIKADFPGFATLIHGEDPERWRRIKPDWGAEFPRIRTKIRFQINIAGFGL
                     IKDSVAKPARQE"
     gene            8763..8924
                     /locus_tag="VF531_10860"
     CDS             8763..8924
                     /locus_tag="VF531_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714509.1"
                     /translation="MKILGITVSPNVLAVILGMVMILFCLAIAYIFQKHLFGRCRRKV
                     PTRAEDDLE"
     gene            9035..>9566
                     /locus_tag="VF531_10865"
     CDS             9035..>9566
                     /locus_tag="VF531_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714510.1"
                     /translation="MRKFFWTVMILSMLASPVLARATVKSSGATVPEPNVVYGEITGI
                     TPTQVILNQSGKSTLWRLTPETRIYCNGLPAVWKALLPVTHQSFFEARIELGARREAT
                     AIHGFYQGEICVLKDWRSRTDGTLEVELTGADSGITRWRCLAPGARVPQASWLAGESE
                     IYVLYNQNEAVRGVYLP"
BASE COUNT         2473 a         2223 c         2316 g         2554 t
ORIGIN      
        1 aagagttatg gcgcgtttca aaaaatcaca ggtcgcctcg gcggtgttct ctcttctgaa
       61 tcccgcggtt atcaaatctt gaaactgccg gcgttcatca acataacacc cttctttgat
      121 gctgtatgta ttattatgat agataatctc cggaaacccg atctttttaa gaatttggcg
      181 taggtaatac aggcaagtgt gaaacaggct ttgggctttt tccaggtcat gctcgggcca
      241 taactcttcg atgattacat cccggctgac tggttttccc tggtgaacca ggagcgccag
      301 taaatcccgg cttttagagg tgcgccaatt gacgcgaacc cccactcctt tttgaaatat
      361 ctggaaaggg cccagcgttt tgaagcaaaa ggcttcaaag gtattctcat tctctgatgc
      421 ccgcaacgat gcaaaccgtg ataacattcc tcgagccgtt tccgctactt ccgtatcggg
      481 gtcttccgtt aacgcctgta atgcggttag cgcctgaccg tcactcaatt ccgagagagg
      541 ggttatggta cgccaccgga ccttggggtc gggatggttt ctcaaagaat tcaacaaggg
      601 aaaagcctgt ttacccaaac gcgccaaaat cgtttggatg aaaggaactt cgatcccttg
      661 ctccaggccg aaacgtaaca ccggctggaa tatatcaaat gaagtcagaa aactttggta
      721 aaaattgcgt ggagccgaaa tctccaaagt ttttttggcg tataaggcgg cagccatatg
      781 gtcgccttct acggtataag acaatgttaa gaaggcatag gcatgcccca ggggccgcgg
      841 aaaacccatt ttttccaaaa cggctgcagc ttccagaacg atttctttgg ccacttgtct
      901 ttccccgtat cgccaaaaga tttgagccgc taattcttga caaagcgcta aaatcagccc
      961 acccggaatc cgggcttcct ccaaagctgc ttcggtgatc aactgagcct cggataatcg
     1021 cccttgcaaa gataaactaa tgccaagata ggccttgttt aaaatgagga agaaatactc
     1081 tccgctataa ttttgagtaa ggtcgatggc ccggcgaaaa tatttctccg ctgtcttcgg
     1141 ttccctgcgg ttgagataaa tatcacccag ttgaaaatat gcggaaggaa gggtatcggt
     1201 caatccgtag ttttctttaa actccacact atgcttggca tattcaaatg cctgttccag
     1261 ctccccccat tcctggtaaa tggcggcgtt atagttctgc atataatagt cgggcatatt
     1321 ttggccggta agtacttccg accgtttcct gtatgcttgc aacgctttgg aataaacccc
     1381 ctgcaaataa taggtattcc ccagaccgtc cagtaaatac gctacggtgt agagggcatt
     1441 ttcattttcc gcgatctcca gggcttgctt taaaaggttc tcggcttccc ggaaacgccc
     1501 gatcatgagc aaacataagg atttttccag aggtaaatca aagcgaagcc gatcccgatc
     1561 attgaggatt tctgtaacat tttccaaaat gaccaggctt tgctgataaa agccctggta
     1621 tcgcaggata cgcgctttaa gtaaacggct ttctgcaagt ccggtccgat cctccaggat
     1681 ggtaaattcg gcaatggcca gattaaccca gtgcgatacc tcttcgattc gtccatgaaa
     1741 ggcttcggcc tgagcctgca acaaagcaag cttggggtcg gcggcctttt ccttgcggga
     1801 gaagaatccc agccagcgtt caacggtttg ccagcgccct ttttgcaagg cttgtggtcc
     1861 ggcgattttt attatggcgc gaatcctgct tgccgtgccc gaagcgatat aatactcgat
     1921 tgccttggct aaatctccgt ttttttcggc aatttggcct gcttcacatt gcagctggct
     1981 tttagccgga ccaagtcgtt cgagtaggaa ctcccggaat agttggtgat aacggtaggt
     2041 tttttccgtc cccgcgagcg gcgtcaaaaa taaattttgc ttttccagcc attttaaaat
     2101 cttggccgaa tcttttcgtc cgcgcaacac gtcgctatcg gagacgctaa ttgtttcgaa
     2161 gaccgaggtt ttaatcaaga attcccgtat ttcctcgggc tgttgttcaa atacttcttt
     2221 ggcgaaataa tcgtaaatcc ccatattcgt ctcatgcaac tccagggaag cagcggtact
     2281 ggagacggcg ccgactgcca gccggaatgc ggccggccaa ccatgtactt tgctattgag
     2341 tacggcagat gactcaactg aaagatgagg agcgtttttt aacagaaatt cattgacttc
     2401 aagatcaata aatgaaagat cttcagctcc gatgactaga atctcatcgg caacctgcaa
     2461 acgggtaaag gtaaatagag gcgcgacccg tccggcaatc atgatatgaa catggctggg
     2521 aagatactcc aaaaattcct ggataaaacg aaataccagc ggtgaggtga tctcatggaa
     2581 gtcgtccaaa acgaagatga tcggctcact ggcaactctt atgagggaat tgataaattt
     2641 tgtaaggatg attcgcaacc tggtttcgat attttcctgt tccagcaata acagaaattg
     2701 ttccccgaaa ttcgggtaat attgttgcag gcccctggtc aaatattgta aaaaaatagc
     2761 aggatcatta tcgtaggcat caatctgata ccaaaccacc ggctgttcca tgacagcgat
     2821 aaattgggcc atggcgacgg tcttaccgta tccggcgggt gcaatcagaa aagtggcttt
     2881 gcgagtttgg atatctccca ttttttggag cagtcgcggt cgttcgatga tgcctttttc
     2941 cattcgtggg ggaatgagtt tggagagaag cagttcagta atgatggacc cttcttttag
     3001 agatattgtc ctttttcctt aattatatca gtttacattc gttaattaaa ctctaaccat
     3061 tttcgtaccc aaaaatcata aacattggtt tgcggtaatt aaaaaagtaa ccggttatcc
     3121 cagttatcgg gttctttata gatcccaacc ggggtacttt ttttatcccc ctaatttttt
     3181 gttgagtttt tgttcagagt cattatttat aatcaaaaat gaatctgttt tacagttctt
     3241 aatggaacat ccaatcttga tggtctataa aagttttggc taaaaagggg ggataacttc
     3301 agttaatgtg gttttttatt atgaaaaaat atcttgagga ggtttatcga atgtttctaa
     3361 aaacttttaa gaagtcgttt attttcgggt tgttattgct tgttgcgatt ctaatgatga
     3421 ccggctgcgg aggaacttca aacccgggtg gttcgaatcc tggtgattca gatccggggt
     3481 cagatccagc cggcgatggg ccatcgtctg cacttatcgg cacttggaat tcctccggca
     3541 tcaatccctc cattcgggtt actcttgggg aatgccttgg aagtataaat aagccggaat
     3601 taaatgtcgt tgaatattac tacaaagggg aagtaagttg cagcgtttta aaagacggta
     3661 aaaaaacaat ttcaacgatg ccgagtttat cggatataga ttctatctgc attcaaaaga
     3721 gtcttgacag tgaagtcttg attatcatgg ccggattatc agacgatagt tcgggcgatc
     3781 tttcggtttc cggctatatt gaaagcaacc aattaaaagc agtagtttgc atagatgacc
     3841 ctagtggcaa tacgatttac gcaactcctg agggatccta tgatatcttc accaagaatt
     3901 aattttgatg aacaacagcc atacttgcgg gttccatagg tgcttgaaat cgtttgaagg
     3961 aggctcgatt catattgtcc aagaagtcac agaaaatatg ggtacttatg gtattacttt
     4021 attccgctgt tcttgtcatg tccggttgtg gaggcaacag caatccgggc tccgacgatt
     4081 caagttcatc cccgggcggt gacgtctgga gatcaacgag tattacgaat tatccgattc
     4141 atcattagcc atgtgaaccg gaaaatattt taatatccaa tcgtctgttt gtatattctt
     4201 ttttttctct ctcatactaa caaaatagaa atcgggggag ataaattgaa tcattggaaa
     4261 gaagccgcgg cttcccggga agccgcaaca tcccgggaag aaacgccgga tgcattatcc
     4321 gaacactatg gcaccaattt gagcagattt cgagagctgc tggggaccag cgcggatctg
     4381 gtgatacgcg aatttaccat tgacgctgaa aaagttaggc aggcggccat tatttattta
     4441 gaaaacttgg ttgccaaaaa ccggattcat gaggatatcc tccggccgct cacggtaaat
     4501 gccgctacaa ttggggcacg gaatatcaaa ccgaaccaaa ttaccgcgtt tctacaatct
     4561 caattggtaa cggtaagccc cgtcgaacag gaaaggacat tcagcaaagc ggtcgaccgg
     4621 atcttttcgg gagatacaat cctaatgata gaggggatta atgaggcgtt catttttgct
     4681 acccggggct gggctcagag ggaggttggt aaacccgaaa gcgaggagac cgtcagaggg
     4741 ccgcgtgagg gatttgttga gacgcttggg acaaatgtag cactgttacg ccggaggatt
     4801 caccattccg atttgatggt cgaggcacat caggttgggc agaagaccaa gaccgccatc
     4861 aacatcgttt atctgaaagg ggttgtcaat gaaaagatag tggccgaaat caggcgaaga
     4921 ctggcacgga ttcaaaccga cgggatcctg gggagcggat atatcgagca gctcatcggt
     4981 gatgcgccca tttccatatt ttccaccacc agttatagtg aacggcctga tgcggttgga
     5041 gcgaaaatcc tggagggccg ggcggcgatt ctgatcgacg gcagcccgga ggttttgacg
     5101 gttccggcct tgtttatcga aagttttcaa aatccggatg attataattc acatccgttt
     5161 tacgccacac tcatccgctg ggtgcggtat atcgcttttg ccgtcagtat ctttgctccg
     5221 gcggtataca tcgcgctggc atcctataac caagaactga tccccacccc tctatttata
     5281 acaatggtgg ccggagccga ggtcactcca tatcccacct ttattgaagt cgtcatcatc
     5341 ggtctacttt ttgagatctt ccgggaggca ggcattcgtt ttcccagacc gtacggacag
     5401 ggaattattt ttgcagtggc ttttgttttg gctcaagccc cagtgaccat ggggttggtc
     5461 ggggaagcta ccgtggttgt ggtggcactt actgccatgg catcgctcgt tgtcccaaag
     5521 caagcggatg ccagcatcct tatccgcttg ctgttggcta ttcttgccag tgtccttgga
     5581 gcctacggca tcctgcttgg actcctgggg gtattggccc acctggtatc cttacgctct
     5641 ttcggtgttc cttatctggc cccgattgct ccttttaata tccgggattt taaacaagat
     5701 gtcatccagc gtacgccttt atgggccatg ttgttccggc cgcgaaccat cggctggcat
     5761 gatcccaaac ggcaaaagag cgagttgacc cctgctccgc cggatagttc ggaagaaggt
     5821 caataggcaa cagggaacag acaacaggca acagaaaaca agtagaagtt cggcttgggg
     5881 cgtaagagag gggattgttt taggctgaaa gtaaccccta gcctggttgc tgatctatgt
     5941 ctttgcttca tcaaaatacc tataattctg ttgcctgttg cctagcttct gttgcctaac
     6001 gtccccgccc cccttttccc cagaatcaag gcggtgagaa ataggaataa ttccagtccg
     6061 ctttcaacca atatgccgat aatcgggaaa acgtagaaaa tgaagttaag ataaacggta
     6121 ttattgggga atgaagttac ggagagcaag aaagcgatca gtgccaccgg atagaccaat
     6181 ggtttatagg tatttagccc cactatctgc gcgataccca ggctgaagat ccagagcgca
     6241 ttgactgccg tcataatccc cgccccgatg gcaatgacta tccaaaccac ttccattcgt
     6301 tcaaagtagg ttgatatatg aattaagcgg gtaagttgca ggccggggtt gaccatattt
     6361 ccggcttgct cggcaccaaa gacaccggtg cagattagaa ccagtatatt gaccatgacg
     6421 agccccatac taacagcgct aaatttagcc agaaacccat tctccgggcg attacagacc
     6481 gcgctatacc agcccatgga gacacagata ccgataaagc ttaggataag cggcgtgccg
     6541 cttaagaaag gataaaatcc ctggtctagt tggggtttca gccgtatcag gtgggaaaaa
     6601 gggagggaca gtaaaaaaga gatgatgata cttcccaagt ataatgggcc caagatttcg
     6661 catattcggg cattcacctc aatccctttc cggacaatat aagctgtaac catgaaggca
     6721 ccgaggagaa tcacttcatt aggggtttgg ggcaggagaa tgttgatgat ctgcatactt
     6781 aggccgcgca tcagaatggt gctgacaacc aaataaaata agatgaagag cagtcccgcc
     6841 acttttccca aaaattttcc aagaatagtg ctgctatact gaaccggatt ttcgcccggg
     6901 aaacggattc ccatgtaagc gtataccagc gccagcatga catcaatgaa ccaagcggca
     6961 atcactgaca accaggcatc acgtccggct tggaagatca ccagtcccgg tatttgaatc
     7021 gcagtaaaag aggtgataat gacgaagagc atccaagtat actgtttggt attgatgatt
     7081 cccgcttccg gaatcggcgc catggtctgt tctcctaatt tattttggta cggtatatct
     7141 tcttaaaatc atctaaaaaa tgccctgccc aggggacgaa gccactgttc gagcagtcct
     7201 atagggtcgg tagttcaaga gccttcccga tgtcaatcag taaggcgatt ccgatcaacg
     7261 ctcccaccac catcaactcc cgccactttt tttcacgggc taacggtatt ccctcaaaaa
     7321 aaccgatgat gacggctaaa atgatgacta gcttcagcat tgctcgttac tcctgccggg
     7381 cgggctttgc gaccgagtcc tttatcaacc cgaaaccggc gatgtttatc tgaaaacgga
     7441 tttttgtccg tatccgggga aattccgcac cccaatccgg tttaattcgg cgccagcgct
     7501 ccggatcttc accatggatc aacgttgcga aaccagggaa atctgccttg atctgttgcg
     7561 ctttttgaat cgtccgttcc aaccggtttt tcagaatact ttcaaccctg cgttcgagct
     7621 gctgaatggt tcggggatta tttttcaaac cttcggactc gcgtagtgta cccgttccgg
     7681 tacagaagat gtccatcagg attccctccg gagtgatacg ggggtaaatc ttggtgcttc
     7741 cgttcattat cgccacagaa attgtttttc ggcctttccc ggtttggaag gggaatacca
     7801 atgaaccgcc cttttgttta tctgccagcc acagcaggcc ttgggactct tccacggtca
     7861 agacgtcgat gagccggtca tttttgaaaa cggcagtcct ttcaagctgt attcctcctt
     7921 gaccgggatc ggttccgggt gctccaacgg ccaccatagg catcaggctg gtttttcctt
     7981 cactgtacat cccggttaag aaatcattga gtgttaccgg tagaaaaaaa gctctcttga
     8041 ccagattgat catcatttga ttgatttctt gcgcgggata tgatccgagg ccggtttttg
     8101 attgtaaaat ctcccgggca ttcgcttccg ccaccaggag ccagttggtt ctgcgaaact
     8161 gggaatctcg tgccatgcag tcaaggatac cggcaatccc cgactcagcc agtgtttttc
     8221 cgaaaatgat gatgcggtta tgaaaaaata tgacccggcg cgaggagtat tttaagaagt
     8281 tacggatcgc atcgtcaaag gacctcccgg tactggtttc gatcacgata gaaccagtcg
     8341 cttgtgaagt acctccggta gttcccgttg taccggatcc acttgttccc tggttgccgt
     8401 ttttggaggc cgcagggttg atgacctgcg ccgttaaaag aatggcccca ttgccgggta
     8461 tcttgtcaat ccctatgccg atgacgatgg cttgttcttt gatttcgctc tgatcccaac
     8521 agccgctgag gaataaaagt agaataattg gaaacatcac gcgtaaatgc aaacgcaaca
     8581 taatcgaatg aaactccctg catcttttat cgactgggtt aaagtagtgt ttgccataat
     8641 tgagaaaata tgcggaccgc atccggtaga taggaggcta tgaaaaatcc aacaatattt
     8701 tccatgaatg aaagcatact atagtagatt caaatccaaa aacccacgag ggatgaatgg
     8761 acatgaaaat tttaggtatc acggtgagtc cgaatgtatt ggcggttatt ttggggatgg
     8821 tgatgattct tttttgtctt gcgatcgctt acatatttca aaagcacctg tttggcagat
     8881 gccggaggaa agtaccgacc cgggctgagg atgatttgga gtgatggtat ccctcccggt
     8941 ccccgcgaga gagcgtcttg agagaatctt ggataaaaac cttttcaagt atgctttctt
     9001 cacaagcggt caataatgat tggggaggag aaagatgcgt aaattcttct ggaccgtaat
     9061 gatcctatca atgctggctt ccccggtttt agcccgggcc acggtcaaat cttccggcgc
     9121 tacggttcct gagccgaatg tggtttacgg ggagattacc ggaatcactc caacccaagt
     9181 cattctgaac cagtcaggaa agagcacatt atggcggctg actccggaaa cccggattta
     9241 ctgtaatgga ttacccgccg tctggaaggc gttgttaccc gtaacccatc agagtttctt
     9301 cgaagcccgg atcgagttgg gtgccaggag ggaagcaact gcgatccatg ggttttatca
     9361 gggagagatt tgtgttctca aagactggcg atcccggacg gatggtacgc ttgaagttga
     9421 attgaccggg gctgattccg gaatcacccg ttggcgttgt ttggctcccg gagccagggt
     9481 tccacaggct tcctggctcg ccggagagag cgaaatctat gttctatata accaaaatga
     9541 agcggtacgc ggggtttatt taccgg
//
LOCUS       DASVMB010000383         7700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_711885,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000383 DASVMB010000000
VERSION     DASVMB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711885"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF531_10870"
     CDS             <1..197
                     /locus_tag="VF531_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX7714511.1"
                     /translation="HSVLEDDCLSRYEFQDYAEVYKVVSEYMNFYNKVRMHGSIRDMA
                     PEVFYLANQQNPIKIAEVRL"
     gene            complement(215..1264)
                     /locus_tag="VF531_10875"
     CDS             complement(215..1264)
                     /locus_tag="VF531_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7714512.1"
                     /translation="MNKEKGNNMQTIIYYSFPVSGHINPVFMTIKKLVDQDIKVICYS
                     TEQFRKQIEAMGATFADYNKEKEYINLNMNLAPDQMTLLSLSRLLIGFLEERFESLLI
                     EAINIKPDGIGLDFFAIWGKMIASSIRIKSFTTIPQFAVNQKRMKPFPGMAKNIIQIL
                     LYGMPEIHRFNLLIKKYDKDYHIGNYKFFDLFVNHTSLNVVYVNKEIQPDIEDFDQTF
                     IFVGPSIRINCDEKFEKIHFPEKYNGKLIYISLGTLYNKNLDFYKKCLKIFTNTSYRV
                     IMSIGNSISLSALGSIPDNIIVKNHVSQINVLKQADVFISHGGMNSVNEALYFGVKLI
                     LMPQAVDQYATAKPG"
     gene            complement(1502..3241)
                     /locus_tag="VF531_10880"
     CDS             complement(1502..3241)
                     /locus_tag="VF531_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HEX7714513.1"
                     /translation="MDLVKLTIDGQSVEVPAGTTILEAAKKAGINIPTLCYHPDLDTR
                     AMCRVCMVEVVGARTLQTACSQPVAENMVIHTSTPAVRDARKLNLELLLSNHPQDCLN
                     CVRNGKCELQALSEQLGVRSQRFPAQEKVAKPKDTSSPSIVRDPAKCILCRRCVAVCH
                     QVQGVGAIYPINRGIETEIAPAGGGKLGDVACTFCGQCIQVCPVGAIYEVDDTAKVWE
                     ALANKDLHVIVQTAPAIRAAIGEEMGMAPGSLVTGKLVAALRRLGFAKVFDTDFSADL
                     TIMEEGNELLSRIQNKGTLPMITSCSPGWIKYIEHFYPDMLGHLSTCKSPQQMFGAMA
                     KTYYAQKMGIPAEKIFMVSIMPCTAKKYECKRPEMQDSGFADVDVVLTTRELGRMLRE
                     TGISFENLPDEKFDDPLGISTGAGVIFGATGGVMEAALRTVYEVVTEKELPKVEFEDV
                     RGMEGIKEATVDLAGTKVKVAVAHGLSNARKLMDKIKDGSADYQFIEVMCCPGGCIGG
                     GGQPIPTNLETRLARMKAIYEADKGMPIRKSHENPAVQELYKEFLGKPLGEKSHHLLH
                     TEYTQRGKYHLVK"
     gene            complement(3278..3637)
                     /locus_tag="VF531_10885"
     CDS             complement(3278..3637)
                     /locus_tag="VF531_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX7714514.1"
                     /translation="MKSLEELAKVREEAKKLTELREGKEGVQIIIGMGTCGIAAGARE
                     TLMAFLDELGKRNIQNVTITQTGCIGLCEQEPIVEVEIPGKGKVRYGKVDAERARRIV
                     ANHIVNGNVLQELVLSI"
     gene            complement(4394..6181)
                     /locus_tag="VF531_10890"
     CDS             complement(4394..6181)
                     /locus_tag="VF531_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HEX7714515.1"
                     /translation="MINLTINQKPVSVAEGTTILEAAAQNHILIPHFCYFEKLHQIGS
                     CRICVVEVEGAKALQASCMVTVQEGMVVRTNTARVKQARKLLYELMLSDHPHDCLNCS
                     RNQNCELQKLGELLGVSEDRFVGEKSKTLKDESSPSIVRDNSKCILCRRCITVCNQIQ
                     GVGVLNAQKRGFKTVIGPAAELPLNSVNCAYCGQCTTVCPVGALGEKDSTGAVWDALH
                     DPKRRVVIQTAPAIRAALGEEFGYEPGTLVTGKMVTALREMGFAEVFDTNFAADLTIM
                     EEGHELLRRLEENHKGTAGKTLPLITSCSPGWIKYVEHAYPDELFHLSTCKSPHMMLG
                     ALIKSFYAAKLGIDPQELFVVSVMPCTAKKFEITRPEMVNNGCPNVDAVLTTRELARM
                     IKNSGIDFGSLADGEFDEVLGLSSGAADIFANTGGVMEAALRTVYEVVTGRELPFDKL
                     HVTPLMGLSRIKTAELTITEPRFEYGFLDGVTLKVAVTSGLAGADRLLKEVASGESPY
                     HFIEVMGCPGGCISGGGQPRPTTDQIREKRLAAIYSEDEAKPLRKSHENPRITTIYQE
                     FLGKPLGEKSHELLHTHYTPRGIFNEKVK"
     gene            complement(6184..>7700)
                     /locus_tag="VF531_10895"
     CDS             complement(6184..>7700)
                     /locus_tag="VF531_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7714516.1"
                     /translation="TAPKPVISMIKYFREEYEEHIRDKHCRAGVCGDLFISPCQNACP
                     AGVNVPGYIALIAAGRFRDAYNLIRKENPFPAVCGRVCTHPCEKKCRRAQLDEPLSIA
                     DLKRYVADTALQNEEPYLDLVFPKKGRKVGIIGAGPSGLTCGYYLARLGYEVEVFEAQ
                     PVAGGVLAFGIPEYRLPKEILKREIKLIEQVGVEIHLNTEVGRDISFEQLRKDFDTVY
                     IATGTQFSRKINIPGEELPGVYHGLAFLRDQNLGGEVKTSERVTVIGGGNTAIDAARV
                     ALRLGAKEVTVLYRRTIEEMPADAREIKDALAEGVRIIPLVAPVRIIGSERVDAVECI
                     RMEPGPFDVDGRRTPQAIPGSEFTIETGMVIPAVSQYSDLPFINRDEVELTRWGTFIT
                     DRDTLMTRMKGVFAGGDVVRGSDVAISAIADGKKAAQAIDKYLGGQGVLNTGEEIPIP
                     AASEEPEILEHERFPMKFLDPEERKTNFNEVAVGFHKLNAIAEAMRCLRCDRRV"
BASE COUNT         1816 a         2114 c         1787 g         1983 t
ORIGIN      
        1 tccatagcgt cttagaagat gattgtttgt caagatatga gtttcaagat tacgccgagg
       61 tttacaaagt cgtatccgaa tacatgaact tttataacaa ggtccgtatg catggaagta
      121 ttcgtgatat ggctcctgaa gtattttact tagccaatca acaaaatcct ataaaaattg
      181 cagaggttag actatagtct ccaatgcgga ggggttaacc cggttttgct gtcgcatatt
      241 gatctacggc ttgtggcata agtattaatt ttacgccaaa ataaagtgcc tcatttacac
      301 tgttcattcc accatgagat ataaatacat cagcctgttt caatacattg atttgagaga
      361 catggttttt tacaatgatg ttatcgggaa tactaccaag tgccgataga cttattgagt
      421 tgccaatcga cataatgacc cgatatgatg tgttcgtaaa tatttttagg catttcttat
      481 agaaatctag atttttgtta tacaatgtac ccagagaaat atagattaat ttgccgttat
      541 acttctccgg aaagtgtatt ttctcaaatt tctcgtcaca attaattcta attgatggac
      601 caacaaatat aaacgtttga tcaaaatcct caatgtctgg ctgtatttcc ttatttacgt
      661 atacaacatt caaactcgta tggttgacga ataaatcaaa aaatttataa tttcctatgt
      721 gatagtcttt atcatacttt tttattagca aattgaatct atgtatttcc ggcataccat
      781 agagcaatat ctggataata ttcttcgcca tacccgggaa aggcttcatt cttttttgat
      841 tcactgcaaa ctggggaatc gtggtgaacg acttaatccg gatcgatgat gcaatcatct
      901 tgccccaaat tgcaaaaaaa tctaaaccga taccgtccgg cttgatattt attgcttcaa
      961 tcaataacga ttcaaatctt tcctccaaaa aaccaatcag gagccgggat aaactcaata
     1021 atgtcatttg atctggcgct aaattcatgt ttaagttgat atattctttt tccttgttgt
     1081 aatccgcaaa agtcgctccc atagcttcaa tttgctttct gaactgttcc gtactataac
     1141 agattacttt gatatcttgg tcgactagtt ttttgattgt catgaatacc ggattgatat
     1201 gtccggatac tggaaaacta taataaatga ttgtttgcat attgtttcct ttttccttat
     1261 tcaaaagtac atcctccatt tattctgtat actcgaggtt tcaacaaagt taattcccat
     1321 atgattaata attctaaaaa ctgcaaaaaa cttcctgcct aaaaatgtaa atcatataaa
     1381 tttgaaaatc taataatttg atgcgtataa ggaacgtaga ccaaaggatt ttcacagatg
     1441 agaaaaggcc gcaagcaaaa tcgcttgcgg cctttgattc aacagctata tagtaatcag
     1501 attacttcac taaatgatac ttgccccgtt gggtgtactc ggtatgcaac agatgatggg
     1561 acttctcacc caagggtttg ccgaggaatt ccttgtagag ttcctggact gccgggttct
     1621 catgggactt gcggatcggc attcccttat cggcttcata gatcgccttc atccgggcca
     1681 agcgggtctc gaggttggtg ggaatcggtt gcccgccgcc gccgatgcag ccgccgggac
     1741 aacacatgac ttcaataaac tgataatcgg cgctgccgtc cttaatcttg tccatcagct
     1801 tccgggcgtt ggacaggcca tgggccactg ccaccttgac tttggtgccc gccagatcca
     1861 cggtggcttc cttgatgcct tccataccac ggacatcttc gaactccacc ttgggaagtt
     1921 ccttctcggt gacaacttcg taaaccgtac gcagggcggc ttccatgact ccgcccgtag
     1981 cgccgaagat cacaccggcg ccggtggaga tgcccaacgg atcatcgaat ttttcatccg
     2041 gcaggttctc gaagctgatt ccggtttccc ggagcatccg gcccagttcc cgggtggtca
     2101 acaccacgtc cacatcggca aagccgctgt cctgcatctc gggacgtttg cactcgtatt
     2161 tcttggctgt gcacggcatg atcgatacca tgaaaatctt ctccgccggg atgcccatct
     2221 tttgagcgta ataggtcttg gccatcgccc cgaacatctg ttgcggcgat ttacaggtgg
     2281 aaagatgacc gagcatgtcc gggtaaaaat gttcgatata tttgatccaa ccagggctgc
     2341 aggaggtgat catcggcaat gtgcctttgt tttggatccg ggagagtaac tcgttccctt
     2401 cctccatgat ggtcagatcc gccgagaagt cggtgtcgaa tactttggca aatcccaacc
     2461 gccgcaaagc tgccaccagt ttgcccgtga ccaggctccc cggggccatg cccatctcct
     2521 cgccaatggc ggcgcgtatg gccggcgcgg tctggacgat cacgtgcaga tccttattgg
     2581 ccagggcttc ccaaacttta gcagtgtcat ccacttcata gatggcgccc accgggcaaa
     2641 cctgaatgca ttggccgcag aaggtgcagg ccacatcacc cagtttgccg ccgcccgccg
     2701 gagcgatctc cgtctcgatg ccgcggttaa tcggataaat agcccccact ccctggactt
     2761 gatggcagac cgccacgcaa cggcggcaca agatgcactt tgccggatcg cggacgatgg
     2821 acggcgagga agtatccttg ggtttcgcca ccttttcctg ggccgggaac cgctgcgacc
     2881 tgacacctaa ctgctcggag agggcttgca attcgcattt cccgttgcgg acacagttta
     2941 agcaatcctg gggatggttg gagagtaaca gttccagatt gagtttacgg gcatcccgta
     3001 ccgccggggt gctggtatga atcaccatgt tctcggcgac cggttgggaa caggccgtct
     3061 gaagagtcct cgccccaacg acctccacca tgcaaacacg acacatcgcc cgggtatcca
     3121 aatccgggtg ataacagaga gtcgggatat tgattcccgc ttttttggca gcttccaaaa
     3181 tggttgttcc cgccggtacc tctacgcttt gtccatctat cgtcaattta accagatcca
     3241 tctgcttcat cctttcatcc aaagattgag accgaaccta tatcgataaa accagttcct
     3301 gcagaacatt cccgttgacg atgtggttgg ccacgatgcg gcgggcccgc tctgcgtcga
     3361 ccttgccgta cctcactttg cctttgccgg gaatctccac ttccacgatg ggttcctgtt
     3421 cacacaggcc gatacagccg gtctgggtga tggtgacatt ttgaatattc cgcttgccca
     3481 gttcatccag gaaagccatc aaggtttccc gggcgccggc ggcgatcccg caggtcccca
     3541 tgccgatgat tatctgaaca ccttctttgc cttcccgtaa ttccgtcaac tttttcgctt
     3601 cttctctgac ttttgcaagt tcttccaatg atttcacacg aattcctccc accagtggaa
     3661 tttgttccgg atttcacaat cttaaatgta taaatagagg accgttaagt cctcaattct
     3721 aaacaaagtt tttacgatgg cagatactgc tcaaaaacta tgtcccaaag tcgctgctcg
     3781 gagattgtta aatgaatatc aaactaaaca accattttta ttatagctga aaacagttga
     3841 aattgcaacc atcacggccg atcggcagat gaaccccttt aaacacggta tatcgtgatc
     3901 cgttttagaa acgctattcc gatacaatta catttcacat tcttttaaca tttatttcag
     3961 tcatattttc tggaatactt ccttctaact cattatttgg gccaacctcc cctatccctg
     4021 cgcgcttagt aaagcgcgac ctcgaggagg ggattagcct aattattgcg gggcaccccg
     4081 ctctccggca gcgtttgcac gctgcacctg ccatcaatat ccagatgcaa acgaaaaaaa
     4141 gaattaacat actcattcgc ggcgtctacg cgcacaaggt gcgcgaagca agcagtgcaa
     4201 ggaacgaccg agcgaggagc acagataata ctctctgtat attgcgcgtg cctggcacgc
     4261 gaagcaccgc agcgaccgag tgacgcggca atgcgccgct tttcataacg ttctttggac
     4321 gttacgcttc agccaatccg gcaaagcttt taatctttcc tcatcactca acagttctct
     4381 aaaaacgaca ctcctacttc accttttcat taaatatccc gcggggggta tagtgagtgt
     4441 gaagcaactc atgggacttc tcccccagcg gtttaccgag aaattcctga tagatcgtag
     4501 tgatccgcgg attctcatgg gacttgcgca ggggtttggc ttcatcttca ctgtaaatcg
     4561 cggccaggcg cttttcccga atctgatcgg tcgtgggacg gggctggccg ccgccgctga
     4621 tgcacccgcc gggacagccc atcacctcga tgaaatgata gggcgactcg ccactggcaa
     4681 cttccttcag cagccggtcg gccccagcca aaccgctggt aaccgcgacc tttagcgtta
     4741 ctccgtccaa aaatccatac tcaaaccggg gttcggtgat ggtgagttcc gcggtcttga
     4801 tccggctcaa tcccattaac ggcgtcacat ggagtttatc aaaaggcagc tccctcccgg
     4861 tcactacttc gtatacagtc cgcaaggcag cctccatcac acccccggtg ttcgcgaaga
     4921 tatccgcagc gccgctcgag agtcccaata cctcgtcaaa ttccccatca gccagactac
     4981 cgaaatctat ccccgaattc ttaatcatcc gcgccagttc ccgggttgtt aatactgcgt
     5041 ccacattggg acaaccgttg ttcaccatct ccggccgggt aatctcaaac ttcttggcgg
     5101 tacagggcat caccgaaacc acaaacagct cctgcgggtc gatacccagc tttgcggcat
     5161 aaaaagactt gattagcgcc cccagcatca tgtgaggtga cttacaggtg gaaaggtgga
     5221 aaagctcatc gggataggca tgctccacat acttgatcca ccccgggctg caactggtga
     5281 tgagcggcag ggtcttgcct gccgtacctt tgtggttttc ttcgagccgc cgcagcaact
     5341 catggccttc ctccatgatg gtaagatccg cggcgaagtt ggtatcgaac acttcggcaa
     5401 atcccatctc ccggagggcg gtgaccatct ttcccgtgac cagggttccc ggttcatacc
     5461 cgaactcttc tcccagggcg gcccggatcg ccggcgcagt ctgaattacc acccggcgct
     5521 tgggatcatg caaagcatcc cacacagccc cggtggaatc tttctccccg agggcgccga
     5581 ccggacagac agtggtgcat tggccgcaat aggcgcaatt caccgagttt aacggcagtt
     5641 cggccgcggg tccgatcacg gttttgaaac cccgcttttg ggcattcaaa actcccactc
     5701 cctgaatctg attgcatact gtgatgcagc gccggcagag aatgcatttg gaattatccc
     5761 gaacgatgga gggagatgat tcgtccttaa gggttttgga tttctcccct acgaaacggt
     5821 cctcactcac ccccagcagt tcccccagtt tttgcagttc acaattttgg tttctggaac
     5881 aattgaggca atcgtgggga tgatcagaga gcatcaattc atatagaagc ttacgcgcct
     5941 gtttgaccct ggcggtgttg gtccggacca ccattccctc ttgaaccgta accatacagg
     6001 aagcctgcag cgccttagcc ccctccacct ccaccacaca gatccggcag gaaccgatct
     6061 ggtgaagctt ttcgaaataa cagaaatgag gaatcaatat atggttctgg gcggctgcct
     6121 ccaggatggt cgttccctcc gctacggaga ccggtttttg gttaatggtc agattgatca
     6181 tctctatacc ctcctgtcgc atcgcagaca acgcatggct tcggcaatgg cattcaattt
     6241 atggaaccct acggcgacct cgttgaagtt ggtcttgcgt tcctccgggt ctaagaactt
     6301 catcggaaac cgctcgtgtt ccaggatttc cggttcctcc gaggctgccg gaatcgggat
     6361 ctcctcgccg gtatttagaa ctccctgccc acccaggtat ttatcaatgg cttgggccgc
     6421 cttcttgccg tcggcaatgg cggaaatggc cacatcggag ccgcggacca cgtctccgcc
     6481 ggcaaatacc cctttcatcc tggtcatcag ggtgtcccgg tcggtaatga aggtacccca
     6541 gcgggtcaac tccacctcat cccggttgat gaagggcaaa tcggaatatt ggctgaccgc
     6601 gggtatcacc atccccgtct cgatggtgaa ctccgatccg ggaatcgctt gcggggttct
     6661 gcggccatcc acatcaaacg gacccggttc catccggatg cattccacag catccacccg
     6721 ctccgatccg ataattcgta ccggcgccac cagcggtata atacggaccc cttcggccaa
     6781 agcgtccttg atctcccggg catcggccgg catctcttcg atggtgcgcc ggtatagcac
     6841 ggtcacttcc tttgctccca gccggagcgc cactcttgcg gcgtcaatcg ccgtattgcc
     6901 gccgccgata acagtcaccc gttcactggt cttgacctcg ccgcccaggt tttgatcgcg
     6961 caagaacgcc aaaccatggt aaactccggg aagttcctcg ccggggatat tgatcttccg
     7021 ggaaaactga gtgccggtgg cgatgtagac agtgtcaaaa tcttttcgca attgttcaaa
     7081 actaatatcc ctgccgacct cggtatttag atgaatctct acgcccacct gttcgatgag
     7141 tttaatctcg cgcttcagga tctcttttgg cagccggtat tcgggaatgc cgaaagccag
     7201 cactcctccg gcaaccggtt gagcctcgaa gacctcgact tcgtatccca aacgggccaa
     7261 atagtaaccg caggtcaacc cggagggacc ggcgccgatg atcccgactt tccggccttt
     7321 tttgggaaag accagatcca gatagggttc ttcgttctgc aaggccgtat ccgccacata
     7381 tcgtttcaaa tcggcgatgg atagcggctc atccagttgg gcccggcggc acttcttttc
     7441 gcagggatgg gtgcaaaccc ggccgcatac cgcagggaac gggttttctt tacggatcag
     7501 attgtaggcg tcccggaacc tgccggcagc gatcagtgca atgtatcccg ggacattcac
     7561 cccggcggga caggcgtttt gacaaggcga gatgaataaa tcgccgcaaa ctccggcccg
     7621 gcaatgttta tcccggatgt gctcctcgta ctcctcccgg aagtacttaa tcatgctgat
     7681 tacgggtttg ggcgctgtct
//
LOCUS       DASVMB010000384         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_638288,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000384 DASVMB010000000
VERSION     DASVMB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638288"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..951)
                     /locus_tag="VF531_10900"
     CDS             complement(1..951)
                     /locus_tag="VF531_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7714517.1"
                     /translation="MSRRKFIPAPNSAPNFIGEHWIPEEWIDVTSLDLTDIDIYEAGA
                     HQCPPGYICGPLVRDHYLIHYILSGKGTYQSGTHSYTLTAGQGFLIFPDTVAIYQADA
                     QEPWNYAWIGFHGLKVEHYLKLTNLSLKNPIFQTANDTPVREYFLEMLDTQKMKKGRE
                     VKLLGLLYLVFSQLMETGGSKFLNDTENPKEIYIKKYIEIVAKNYSRSVNIGQIAQNI
                     GIDRRYLSSIFKERLNLSPQEFLINFRMNKACELMENRQLSISDIARSVGYEDPLQFS
                     KIFKKMTGKSPTDYRKNVIQDPGRQVIGPEFLNQKPPNKQ"
     gene            1228..>3290
                     /gene="gnpA"
                     /locus_tag="VF531_10905"
     CDS             1228..>3290
                     /gene="gnpA"
                     /locus_tag="VF531_10905"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HEX7714518.1"
                     /translation="MMKDKITSGRVTLPAEKGMEHQVALLADQWGADAVRDSDGTKLS
                     PEILEMNFEVYTTLCLIREDQEWIKRHPEYRQQMYLCSDAVITEKETVAIELLRGYFS
                     EQFQIDDVHDPKIWWEVIDRTTGEVVSPAAWSFQREKGLVVINDSQPWHKYTVSFLVY
                     QIWEPVSMYNHITNHWTEEHRMPLDPRHSEAREHMLNYLDQWLGAHPRTDVVRFTTFF
                     YNFDLIYDQNGAEKQVDWFGYLSCVSPLALEQFAEEYGYKLRPEDFIDGGSYNTPFKV
                     PTSKYLDWMEFNQKFIANLAKECVERVHRAGKKAIMFLGDHWSGTEPYGRYFKEIGLD
                     AVVGATGDGVTTRMIADIPVKATEARFYPYFFPDVFHEGGDPVKESLEVWRKSRRALL
                     RRATDRMGYGGYLSLVVKFPDFVEHIAAVCEEFREIHAMSKGTKPYSASFKVGILNAW
                     GSIRSWMTHQVAHSLWNQRCYSYLGILEALAGLPFEVEFISFDDIKKWGIPSDMGVII
                     NAGDAGTAWSGGEFWKDQKVITVIREWVYHGGGFIGMGEPTACQYSGAYFQNAYFRLP
                     EILGVQKEIGYSVSLDKPKIQTVTKHFITDDIRGKIDYGEGMNSIYKCCDTTIVLDTN
                     NLSCSLAVNTFGKGRAVYIAGLPFNIENTRLLSRALYWSAGKEGEMSNWYCDNPTFEC
                     SAYPEA"
BASE COUNT          873 a          657 c          846 g          914 t
ORIGIN      
        1 ctattgctta tttgggggtt tttggttcaa aaactccggc ccaataacct ggcggccggg
       61 gtcttgaatg acgtttttcc ggtaatccgt gggggatttg ccggtcattt ttttgaatat
      121 tttggagaac tgcagggggt cttcataccc aaccgagcgg gcaatgtcgc taatggatag
      181 ctgcctattc tccatcagct cacaggcttt attcatccgg aagttgatca aaaattcctg
      241 gggcgataaa ttgagacgtt ctttaaatat gctggataaa taacggcggt caatcccgat
      301 attttgagca atttgaccaa tatttacact ccgggaataa tttttggcaa cgatttcaat
      361 gtattttttg atatagatct ctttagggtt ttcggtatcg ttgaggaact tagaaccgcc
      421 cgtttccatc agttgggaaa aaaccaggta cagaagtcct aacaacttca cctcccggcc
      481 ttttttcatt ttttgggtat ccaacatctc caggaaatat tccctgacgg gcgtatcgtt
      541 tgcggtttga aaaattgggt ttttcagtga aagattcgtt agtttcaaat agtgttcaac
      601 tttcagtcca tgaaaaccta tccaggcata attccacggc tcttgcgcat ccgcttgata
      661 aatggcgacc gtatccggaa aaatgagaaa tccttgtccg gccgtcaaag tgtaactatg
      721 ggtcccggac tgataggtcc cttttccgct gagaatataa tggattaagt aatgatcccg
      781 gactaaaggt ccgcaaatgt atccgggagg gcattgatgg gcgccggctt cgtagatgtc
      841 gatgtcggtt aaatccagac tggtaacatc aatccattct tcaggaatcc agtgttcccc
      901 gataaagtta ggagctgagt tgggagccgg tatgaattta cgtctggaca tcaatgctgc
      961 tcctttccag cggtttaatg cgtcaaatct accttttata ggacattata ccatgttttg
     1021 attacctttt tccatttgca cagcttgaat gatgtcttag aatttagatg agattttaat
     1081 tgcgtctgtt tagggtgaat ccttgggatt gagcagtttt catgcaatcc tatatatgat
     1141 caaaacgtac gactgtgtat tgacaaatct tccaagttcg gattgggatt ttgaaaccgg
     1201 caaaatttta agtaaggggt ccagtcaatg atgaaagaca aaatcacgag cggccgggtg
     1261 actttgccgg ctgaaaaagg aatggaacac caggtggcgc tgttagcgga tcaatggggc
     1321 gctgatgcgg tacgggacag tgacggcacg aaactttctc ctgaaatttt ggagatgaat
     1381 tttgaagtct atactacatt atgcttaatc cgtgaagacc aggaatggat aaaacggcat
     1441 cccgaatatc ggcagcaaat gtatttatgt tccgatgcag taatcactga gaaagaaact
     1501 gtggcgatcg agcttctgag aggttatttt tcggaacaat ttcaaattga tgatgtgcat
     1561 gaccccaaaa tttggtggga agtgatcgat cgtactaccg gtgaagtcgt ttcgccggct
     1621 gcttggagtt ttcaaagaga aaaaggcctg gttgtaatca atgattcaca gccgtggcat
     1681 aagtatactg tcagcttttt ggtttatcag atttgggagc cggtatcgat gtataatcac
     1741 atcactaatc actggacgga agaacaccgg atgcccctgg atccccgtca tagcgaagcc
     1801 agagagcaca tgttgaatta tctggaccaa tggttggggg cgcatccccg gacggatgta
     1861 gtgcgattta ctaccttctt ttataatttt gatttaattt atgatcaaaa cggcgcggaa
     1921 aaacaggttg attggtttgg atacttaagt tgcgtaagtc cgctcgcgtt ggaacaattt
     1981 gccgaagagt acggttacaa gttaaggccg gaagatttca tcgacggcgg ttcgtataat
     2041 accccattca aggtgcctac ttcaaaatat ctggactgga tggaatttaa tcagaagttt
     2101 attgccaatc ttgcgaaaga atgtgtggaa cgggtacatc gggcggggaa aaaggccatc
     2161 atgttcctgg gggatcattg gtccggcacg gaaccttacg gaagatattt taaagaaatc
     2221 ggactggatg cagtggtcgg ggctaccggg gacggagtta ccacaaggat gatcgccgat
     2281 ataccggtga aagcgactga ggctaggttt tacccgtatt tttttccgga tgtctttcat
     2341 gaaggcggag atccggttaa agagtcattg gaggtttgga ggaaatcccg cagggcatta
     2401 ttgcggagag ccacggacag aatgggttat ggcggatatt tgagtctagt ggtgaaattc
     2461 cccgattttg tcgagcatat tgccgcagtt tgtgaagaat tccgagaaat tcacgccatg
     2521 tccaagggga caaaacccta tagcgcatcc ttcaaggtag gaatactcaa tgcctgggga
     2581 agcatccgtt cctggatgac ccatcaagtt gcgcattcct tatggaacca gcggtgttat
     2641 tcctatttgg gcattcttga agcgttggcg gggctcccct ttgaagtgga gtttattagt
     2701 tttgacgata tcaaaaagtg gggcattcct tccgatatgg gcgtgatcat caatgcgggc
     2761 gatgccggca ctgcttggag cgggggagag ttttggaaag atcaaaaggt gatcacggtg
     2821 atccgcgaat gggtatatca cggaggcggc tttattggca tgggcgaacc caccgcctgt
     2881 caatatagcg gtgcctactt ccagaatgcc tacttccggc tcccggagat tctcggtgtt
     2941 caaaaggaga ttggatattc cgtaagtctt gacaaaccga agattcaaac ggtaacgaag
     3001 cattttatca ccgacgacat ccgtgggaag atcgactatg gtgaaggaat gaactccata
     3061 tataagtgct gtgacacaac gatagtattg gatacaaata acctgagctg ttcactggca
     3121 gtaaatactt tcggtaaagg ccgagccgta tacatagccg gactaccatt taacattgaa
     3181 aacacaaggc ttttgtcgcg ggcgctctat tggtcggcag gaaaagaagg ggaaatgtcc
     3241 aactggtatt gtgataatcc gacgtttgag tgcagcgcct atccggaggc
//
LOCUS       DASVMB010000385         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_638652,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000385 DASVMB010000000
VERSION     DASVMB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638652"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF531_10910"
     CDS             complement(<1..420)
                     /locus_tag="VF531_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7714519.1"
                     /translation="MNQFTYLTVLIALLALTVVFLSYRWFQARKKLADYLSVLKRIES
                     GTENAKIFVHGKGMIADIGFQTNRIIDVYREQIIELKKSQLSNKQLLTSLSHDVRTPL
                     TSLLGYLDAVTNQVATGIEKEEYIRIARNKAYNLKDFV"
     gene            complement(486..1184)
                     /locus_tag="VF531_10915"
     CDS             complement(486..1184)
                     /locus_tag="VF531_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714520.1"
                     /translation="MKQHILVIDDDLDLTQLIKKYLEMENYEVTLTHTGIAGLAEATH
                     RSYLLIILDIMLPGKNGFEVLSELRKTSAVPVLMLTAKDSEVDKVSGLRMGADDYLTK
                     PFSMNELVARAQSLIRRYTKFNEPQGNMGGNLEFGTLSINPQTREVMSGGKAVELTAK
                     EFDLLYFLAKHRGQVFTKKQIYSQVWQEEYAFDDNNIMVHIRRLRKKIEPDAETPTYI
                     QTVWGVGYKFTGRV"
     gene            complement(1253..2104)
                     /locus_tag="VF531_10920"
     CDS             complement(1253..2104)
                     /locus_tag="VF531_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7714521.1"
                     /translation="MSNDQYQSNVRVDLHIHTTASDGTWSPEELVNNLREAGIQLFAV
                     TDHDSTANLAETAALAKEQGLQLIKGVEVNTSYNNRNYHILGLGIDPGDTGLQDLLKR
                     NRDLMGQKDDESIEYLEGRYPGVSLEEYMGYINHPERGGWKALNYLIDQKLCHNHRDF
                     FDLFRDWGNPFERLIFATPQKAALAIKEAGGVPILAHPGAAFYDKDYRTVISYMIEAG
                     IGGIECYHPENSPEITRYCLEVCQSRGLVVSGGSDCHGDFLPARWLGNPDIRLGQLRI
                     DEIKDIQ"
     gene            complement(2125..2418)
                     /locus_tag="VF531_10925"
     CDS             complement(2125..2418)
                     /locus_tag="VF531_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX7714522.1"
                     /translation="MFHKVIQVYPTEDYRVYLYFTDGKVKLFDAKELVIQGIFQQLKD
                     AVIFRETCTVLNDTLAWDLSGKRDPSECLDLDPEELYETCPEVEEPPINTALN"
     gene            complement(2431..2676)
                     /locus_tag="VF531_10930"
     CDS             complement(2431..2676)
                     /locus_tag="VF531_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX7714523.1"
                     /translation="MNAGIKILMLWNEHMPPHFHAEYGDKKILVDIEKGIVIKGVFPF
                     KQLKLVLAWCEIHREELMNNWENALKNEQLYRINPLV"
     gene            complement(2742..>2952)
                     /locus_tag="VF531_10935"
     CDS             complement(2742..>2952)
                     /locus_tag="VF531_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7714524.1"
                     /translation="IQFAETITCPVVAIHGEYDPHPMEGVKLPLENRVRDFTFYQLAK
                     CGHDPWKERYAQERFYEILRKELAE"
BASE COUNT          770 a          762 c          575 g          845 t
ORIGIN      
        1 cacaaagtct tttagattat atgccttatt tctggcaatg cggatatact cttctttttc
       61 gatacccgtc gctacttgat tggtgactgc atccaagtat cctaagagcg aggtcaacgg
      121 cgtcctgaca tcatgagaca aactggttaa cagttgctta ttcgacaact gggacttttt
      181 tagttcaata atctgctccc gataaacatc gataattcgg ttggtttgaa atccgatatc
      241 ggcaatcatt cctttaccgt gtacgaatat cttcgcgttt tcagtgccgg attcgatccg
      301 cttcaagacg gataaataat ccgccaattt ttttctggcc tggaaccaac ggtaacttaa
      361 aaaaacaacg gtcaaggcaa gaagtgcaat taatactgtc aaatacgtga attgattcat
      421 ttacccaatc gctccccagc ctagccgtaa tggcttcatc agcccccaaa ctttgatgga
      481 aacaactaaa cccggccggt aaatttatac cccacacccc agacagtttg gatataagtc
      541 ggtgtttcgg cgtcaggctc gatcttcttg cgtaatctgc gaatatggac catgatattg
      601 ttatcgtcaa aagcgtattc ttcttgccaa acttgactgt agatctgttt ttttgtgaat
      661 acttgtcccc tgtgcttggc taaaaaatat agcagatcaa attccttggc agtcagctcc
      721 accgcttttc caccggacat cacttcccga gtttgaggat tgatagagag ggtgccgaat
      781 tccagattac ctcccatgtt tccttggggt tcattgaact tggtatagcg gcgaatcaaa
      841 gattgggctc tcgccactaa ctcattcatg ctgaagggtt tagtcagata atcgtccgct
      901 cccatccgca aaccggaaac cttatcaacc tcactgtctt tagcagttag cattaatacc
      961 ggtactgcac tggttttcct gagttcggaa aggacctcaa aaccgttttt ccccggcaac
     1021 atgatgtcta aaatgatcaa aagataagac cggtgtgtcg cctcagccag tccggcaatt
     1081 ccggtatggg tcagcgtaac ttcataattc tccatttcaa gatacttctt gataagctgg
     1141 gtcaaatcca gatcatcatc gatgactaag atgtgttgct tcatgggttc agtcctttgc
     1201 cttatccttt actgcctgct aagccaattc attaagttgt ataccaatcg atttattgga
     1261 tatctttaat ttcatcaatc ctcaactgcc ccaaccggat atcgggattc cccagccatc
     1321 tggcaggaag aaaatcacca tggcagtccg aaccgccgct gactactaaa ccccttgact
     1381 ggcagacttc caggcagtac cgggtgatct ccggactgtt ctccggatga taacattcga
     1441 ttccaccaat tcccgcctca atcatataag agataacagt ccgataatcc ttgtcataaa
     1501 acgcggcgcc ggggtgagcc aggatcggaa caccacccgc ctctttgatg gccagcgctg
     1561 ctttttgagg agtagcgaag atcagcctct caaagggatt gccccaatcg cggaagaggt
     1621 cgaagaaatc gcggtgatta tgacagagct tttgatcgat caagtagttc aaagccttcc
     1681 agccgccccg ctccggatgg ttgatgtagc ccatatactc ttcaagagat accccggggt
     1741 atctgccttc aaggtattca atgctctcat catccttctg ccccatcaaa tcccggtttc
     1801 gtttcaaaag atcttgtaac cccgtgtccc cggggtcgat ccccagacct aaaatatgat
     1861 aattgcggtt attgtatgaa gtgttgacct ccacgccttt gatcagttgc aggccttgct
     1921 ccttggccaa cgccgcggtt tctgccagat tggcggtgga atcgtggtcg gtcacggcaa
     1981 agagctgaat ccccgcttcg cgaagattgt tgactagttc ctccgggctc caggtgccgt
     2041 ccgaggcggt ggtgtgaatg tgcaaatcaa ctctgacatt actctgatat tgatcattac
     2101 tcattcgatt caaccacccc tccattaatt caaagctgta ttaataggtg gttcctctac
     2161 ttccggacag gtttcataaa gctcttcggg gtccaaatcc aaacactcag atggatccct
     2221 cttaccactc agatcccatg ccagggtgtc attcaaaaca gtacatgttt cccgaaatat
     2281 aactgcatcc ttgagctgct gaaagatccc ttgaatcaca agctctttag catcaaacaa
     2341 cttcactttg ccatccgtaa aatataaata gactctgtaa tcttctgttg gatacacctg
     2401 aataaccttg tgaaacatat tatcagcccc ttaaactaac ggattaattc tgtataattg
     2461 ctcgttcttt aaagcgtttt cccaattatt cattaactct tcccgatgga tttcacacca
     2521 agctaaaacc aacttcaatt gtttaaatgg aaatactcct ttaatcacaa ttcctttttc
     2581 aatatccact aaaattttct tatctccgta ctcggcatga aaatgtggag gcatatgctc
     2641 attccagagc attaagatct taatcccggc attcaatctc ctccaaacaa tctatatcta
     2701 tatgatatac tggaacgaaa tgcattcaca attaagtaac gctactctgc caactccttc
     2761 cgcaggatct cgtaaaaccg ttcctgcgcg tatcgttcct tccaggggtc atggccgcat
     2821 ttcgccaact gataaaaggt aaaatccctt actctgtttt ccagcggcaa tttcacgccc
     2881 tccatcggat ggggatcata ctccccgtgg attgccacta ccggacaggt tatcgtctct
     2941 gcgaactgta tg
//
LOCUS       DASVMB010000386         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_123219,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000386 DASVMB010000000
VERSION     DASVMB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123219"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            668..2149
                     /gene="gltX"
                     /locus_tag="VF531_10940"
     CDS             668..2149
                     /gene="gltX"
                     /locus_tag="VF531_10940"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7714525.1"
                     /translation="MNQNTVRVRFAPSPTGYLHIGGARTAIYNWLYARHTGGKFLLRI
                     EDTDRHRYVPDALNDIMASLQWLGMDWDEGPEAGGDYGSYFQSERLELYQKYAEQLVD
                     QGLAYRCYCTPERLEALRKSQEEAKTAIGYDRHCLNMTEAERRQCEAGGIRPVIRFKI
                     PREGHTRVRDLVRGEMDFENSTIDDFVLLKSDGYPTYHLANIIDDHLMKISHVMRGDE
                     WLVSTPRHVLLYQAFGWDPPEFAHLPIFLAPGGGKLSKRHGATSVKEYRDKGILPEAL
                     FNFLLLLGWNPGTDQELFTAEEAVKAFTLERINASPVALSFEKLDWFNGVYIRNLTHE
                     DLARRCLPYLAQAHLVPESCPEERLPYLISLMPLLQERLKSLPDITEAAGFFLAEDIP
                     APAKEILIPKKMDQAQTLRALEGVEEVLRGADPEFKEAELERVLRELAVKLELHDGQV
                     FMPLRVAVSGKTATPGIFETLHALGKARVLKRVAAANGILRKG"
     gene            2222..2295
                     /locus_tag="VF531_10945"
     tRNA            2222..2295
                     /locus_tag="VF531_10945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2254..2256,aa:Gln,seq:ctg)
     gene            2340..2415
                     /locus_tag="VF531_10950"
     tRNA            2340..2415
                     /locus_tag="VF531_10950"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2374..2376,aa:Glu,seq:ctc)
     gene            2701..3132
                     /locus_tag="VF531_10955"
     CDS             2701..3132
                     /locus_tag="VF531_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714526.1"
                     /translation="MLALVIVFVPRREIKKLFWFSLLWGSMVDFLLVELFRLLNFYRY
                     ENSFPLEFYGSPIMLNLAWSQAIILFIHFLPRRKEWYVLPFYIAMFSMIGTAIGVFFK
                     NAGLIREIHWNELLRFPVWVVWFYGVCWHYNRLRARDRTTI"
BASE COUNT          788 a          757 c          834 g          840 t
ORIGIN      
        1 cagagtgatt aatgctgtca aatagtttaa ttaggaagag tgagcagcgg gaaaaattta
       61 catcggtggc ttttataaga ccaaatagca ggctttcgag tgtggcggat cgtaccggcg
      121 tcgtttaggt gtgattttat atatcggtaa ttctccattt cctcttgaac ttttaaagat
      181 gcgggcaaac ctttgcccga tgaataaacc ccactttgaa tgtttgttaa gtattacaat
      241 tgataaaaga tgactttgac aaagatagta tttttattga tttgaatgaa gacggaaata
      301 aagaagagta taaattaaaa cagtattata aaagtcaatc ttccgttcca ttacgggctg
      361 aattgtttgc ttttcccaga tgcctggcag tgacgagttc atcaaatcca cccacaaatt
      421 ccgaggagct ggaaccaatt gaagacaaat aaaaccgttt gatcgagtca agacttatcc
      481 agataatccc aataatcctg gtaatcccgt aaatccgtta aatcctggtc atcctggtat
      541 aatcctggtc aaaatatctt gacttcaagt tttacacggc atatgattat acaaggttcg
      601 tcgcgcgaac ggggttcgcg cggctgagaa aagttaccac aaagcagacg ctcaggagtg
      661 agatatcatg aatcagaata cggtccgggt ccgttttgcg cccagcccca ccggatattt
      721 acatatcggc ggcgcccgca cggccatcta taactggcta tacgcccgcc acactggcgg
      781 taaatttcta ttgcggattg aagataccga ccgccaccgt tatgttccag atgccttgaa
      841 cgatattatg gctagccttc aatggctggg aatggattgg gacgagggtc ccgaagccgg
      901 cggcgattac ggttcgtatt tccaatcgga acggttggag ctttatcaaa aatacgctga
      961 acaattggta gaccaaggtt tggcttatcg ctgctattgc acgccggaac gcttggaggc
     1021 gctgcgcaag tcgcaggagg aggccaagac cgccatcggg tatgaccgcc attgtctgaa
     1081 tatgaccgaa gcggagcggc gccagtgcga ggccggcggg atcaggccgg tgatccgttt
     1141 caagattccc cgtgaggggc acacccgggt cagagacctg gtccgggggg agatggattt
     1201 cgagaacagc accatcgacg acttcgtttt gttaaaatcg gacggatatc ccacctacca
     1261 cttggccaac atcattgacg accatctaat gaagatctcc catgtcatga ggggggatga
     1321 gtggctggtc agcaccccgc ggcatgtcct gttgtatcag gctttcgggt gggacccgcc
     1381 ggaattcgcc catctgccga tcttcttggc gcccggcggc ggcaagttga gcaaacggca
     1441 cggcgctacc agtgtcaagg agtaccgcga taagggcatt ttgccggagg cgttattcaa
     1501 cttcctcttg cttttgggct ggaatcccgg gaccgatcag gagctgttca cggcggaaga
     1561 ggcggtaaag gcctttaccc ttgagcggat caacgcttcg ccggtggccc tctcctttga
     1621 gaagctggat tggttcaacg gcgtttacat ccggaatctg acccatgagg atctggcccg
     1681 gcgctgcctg ccgtatctgg cccaggctca tctggttccc gagtcctgcc cggaggaacg
     1741 tttaccgtat cttatttctt tgatgccgct ccttcaggaa aggttgaaat ccctgcctga
     1801 tatcaccgag gccgcaggct ttttcctggc cgaggatatt ccggctcccg ccaaggagat
     1861 cctgatcccc aagaagatgg atcaggccca gacccttcgg gctctggaag gggtggaaga
     1921 ggttttgcgg ggagcggacc cggagttcaa agaggccgaa ctcgaaaggg ttctgcggga
     1981 acttgcggtg aaactcgagc ttcacgacgg ccaggttttc atgccgctcc gggtggcggt
     2041 ttccggtaaa acggcaaccc cgggaatttt tgaaaccctg catgctctgg gaaaagcacg
     2101 ggttctgaaa agggtggccg ctgccaacgg gatattgcgg aaaggataat gatccgcatc
     2161 ttggcagaaa actttctctt gacggaaccg gcaaatgcct gttataatat ataatgcgcg
     2221 ttgggggatc gtctagtggt aggacaacgg gttctggccc cgccagcgag ggttcaactc
     2281 cttctccccc agccaaattc agttaacagt aatcacttaa aaactgaaaa ctgaaatacg
     2341 gtcttatcgt ctagaggcct aggacgctgc cctctcacgg cagaaactcg ggttcgaatc
     2401 ccgataggac taccaaaaga aacaaaaccc tgttaaacca gggtttattt ttttatctca
     2461 cacaaaatgg atgggcggta agaaggatcc tttgcggaag gaggttgatt ttttatcccg
     2521 atcttttgac agcgcggatg gagcttaaac attgaccggg aacggtttta tgaaaaaaaa
     2581 cttaagcgct tccaaaaaga tccgtggaat ttttcgctag gtccgtcaaa caataaactt
     2641 aaaaagatcg ggaggccctc catttctaac cagatttatt tcattgcttt cgccctgagt
     2701 atgctggcac tggtgatcgt ctttgtcccg cgccgcgaga ttaaaaagct cttttggttc
     2761 agcctgttgt ggggcagtat ggtcgatttt ttactggtgg aactgttcag gctgctcaat
     2821 ttttaccgtt atgaaaactc atttccgctt gagttttacg gctctccaat catgctaaat
     2881 ctggcgtggt ctcaggcgat tattctattt attcattttt tgccgcgacg caaagaatgg
     2941 tatgtgttgc cgttttatat tgccatgttt tcgatgatcg gtactgcgat tggtgtattc
     3001 tttaaaaacg ccggcttaat cagggaaatc cattggaatg agttgctccg ttttcctgtt
     3061 tgggtagtat ggttctatgg ggtttgttgg cattacaacc ggctgcgggc tagagatcga
     3121 acgacgattt aatatctact ttaaccgctc taccactttg atttcgggat actgatatag
     3181 gttataggtc tgcccctcgc tcacgccacc caccacttt
//
LOCUS       DASVMB010000387         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_272271,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000387 DASVMB010000000
VERSION     DASVMB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272271"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1494
                     /gene="tyrS"
                     /locus_tag="VF531_10960"
     CDS             271..1494
                     /gene="tyrS"
                     /locus_tag="VF531_10960"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7714527.1"
                     /translation="MSVFQTLQERGFIQQTTHQEPLYELLEREKVTFYIGFDPTADSL
                     HVGHLLTIMAMAHMQRGGHRPIALLGGGTAMIGDPSGKTELRKMLTQETIAQNAACFK
                     KQLARFIDFANGKALMINNGEWLLNLNYIEFLREIGSKFSVNRMLTAECYKARMERGL
                     TFIEFNYMLLQSYDFLMLSRKHDCKIQMGGDDQWSNILSGADLIRRLDGKEAYGVTFP
                     LLTTASGKKMGKTESGAVWLDPAKTSPFDFYQYWRNVEDQDVARFLALYTFLPMEEVR
                     RLGVLRDQQINEAKKVLAYEVTRLVHGEEEAGKARQAAEALFGGSGNGQTAPGIDLPR
                     TELAQGMALIDLLVRVGLADSRSDAKRLITQGGVTVQDQKITDLGRVITPDDAADGQI
                     MLRKGKKDFQVVRLV"
     gene            1780..2970
                     /locus_tag="VF531_10965"
     CDS             1780..2970
                     /locus_tag="VF531_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7714528.1"
                     /translation="MTGLVRKTPVLLPAVSKGFLKPEYWLRKQKDVEIGPCFAGVKPG
                     VAERIAAYRRMVRQMMSGTYYDLSGSRFDWEPWQRLDATLDSLPGLQSGLIVAPAELK
                     ILPQKEVLLSDGRVVDLDRNQISGLDPGDWCIVFAATPGEKWYGVLTRQGTGWVPEKA
                     VALCSKTEVDSYWKFQPRLMVLDPAAKVFTNGGTLPISMGSSLPVKNFADSLVVLPQR
                     GSDGKLRLETVMLKGQFCMEKLPATRHNLIRQAFQYLGYRYAWGDFDCEGRGRDCSRV
                     VQDAFSTLGLKLPRYSQEQLAAGLNRIDLAGRGEFERRELLDCCGLGDLLFTNSHVLI
                     FLGKEAGRYYALHAFYKYQAPVPMGEVTKTVKQVVVSDLSPGEGTSTGSLLQRLTGVV
                     SLLT"
     gene            3128..3592
                     /locus_tag="VF531_10970"
     CDS             3128..3592
                     /locus_tag="VF531_10970"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX7714529.1"
                     /translation="MEQILVTAAVIAEPGRGILLAQRLPYGPEGGKWEFPGGKLEPGE
                     SPQSCLSRELREELGVAATVGPVLDIVSQNRENRQLILAYFLCRIDSGVPAALESQAV
                     KWFDPQQILSLDLSGADREFWENTRRNRQEWVTALTVIGPGSSGRFGSVPRS"
     gene            complement(3528..>4253)
                     /locus_tag="VF531_10975"
     CDS             complement(3528..>4253)
                     /locus_tag="VF531_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714530.1"
                     /translation="NLGGPDLEILKLDLAQRLIYLDPGERFWTLHRVEAEYYLGAENC
                     TKRGDRILTLTFAVGGAGAQKEIGCEILKSLRASILNRRIRLNLVAGVRQEIHDYYHK
                     EAVALGLDGELGTGLNILYSPNREKYFQRFNLMLRTTDILWTKPSELSFYCGLGLPII
                     IAPPLGAHEHFNKKWLIEHRAGIAQEDPALTAEWLFDLLNDGQFAQAAWDGFLNARKC
                     GTFKIEELIRTGRMTRDQSPLKR"
BASE COUNT          990 a         1039 c         1239 g          985 t
ORIGIN      
        1 cgcggctgac aatcagagag cgggtgaagg ctgcgaaccc gccgtacacg gtaagaagtt
       61 acactccgga gcttccgaat cgaaacaacg gtagacagct gaaagcggtt atccgcagta
      121 tctggttgac gccttgagta gatcggtacg ggttcgccca ttacagcgct ataagtcgcg
      181 ctgctttgaa cgcgcgaaaa ttaaggtggt accacgagac tctcgtcctt atccggatga
      241 gggttttatt attttaaagg aggagtttcg atgtcggttt ttcaaacttt acaggaacgg
      301 ggtttcatcc aacaaaccac ccatcaagaa ccgttatatg aactattgga gcgggaaaaa
      361 gtcacatttt atatcgggtt tgatcccacc gcagacagtc ttcatgtagg ccatttatta
      421 acgatcatgg ctatggccca tatgcaacgg ggaggacacc ggcctatcgc ccttctgggc
      481 ggcggcacgg cgatgatcgg agatcccagc ggcaagacgg aactgcgtaa gatgctgacc
      541 caggagacta tcgctcaaaa tgccgcgtgc ttcaaaaaac agctcgcccg gtttatagac
      601 ttcgcgaatg gaaaagcgct gatgattaat aacggcgaat ggctactcaa cctgaactat
      661 attgaatttt tacgggagat tggttccaag ttctcggtga accgaatgct gaccgccgag
      721 tgttacaagg cgcgcatgga acggggactt accttcattg agtttaacta tatgctgctc
      781 cagtcttatg attttttgat gctctcccga aagcatgact gcaagatcca gatgggcggc
      841 gatgatcaat ggtcgaatat tttgtccgga gcggatctta ttcgccggct cgacgggaaa
      901 gaggcttacg gagtcacttt ccccttattg accaccgcca gtggcaaaaa aatgggaaag
      961 accgagagcg gcgcggtttg gttggatccc gccaagacta gtccctttga tttttatcaa
     1021 tactggcgca atgtcgagga ccaggatgtt gcccggttcc tggctttgta tactttcctg
     1081 cccatggaag aggtacgccg cctgggggtg cttcgggatc agcagattaa tgaggccaag
     1141 aaggttttgg cctacgaagt gacccggttg gtccacggtg aagaggaggc tggtaaagcc
     1201 cgacaggccg cggaggcact cttcgggggt tccgggaacg ggcagacggc gccggggatc
     1261 gatctacccc ggaccgaact ggctcaggga atggccttga tcgatctctt agtccgggta
     1321 ggactggccg attcccggag tgatgccaaa cgtttaatca cccagggcgg ggtgacggtt
     1381 caggatcaaa agataaccga tctcggccgt gtgataaccc cggacgatgc cgccgatggg
     1441 cagattatgc ttcgtaaagg caagaaggat tttcaggttg taaggctggt gtgaaatagg
     1501 cgtgaggcgt gaggcatgag gcatgaggca tcgtgccctg caggacgcgc agcaacaggt
     1561 aacaggcaaa aggcaaaagg cagtaggaac agacacattg cggggtgaag ggcgaaaata
     1621 gatttcaaac ttcaaacttc acccttcaaa cttttcgtta taccggttcc ccttttccca
     1681 ccgaatttca ttaattatag tggacctccg aaaacccggt tggatgggtt tcgcgatggt
     1741 cgaaagatat tcgtcgggta aggaaagggg aggaagccga tgacgggatt ggtccgaaaa
     1801 acaccggtat tgttacccgc agtatcgaaa ggttttctca aacctgaata ctggcttcga
     1861 aaacaaaagg atgttgagat cggcccatgt tttgccggcg tcaagccggg agtggcggag
     1921 cgtatcgccg cataccgccg catggttcgg cagatgatga gcggaacata ctatgatcta
     1981 agcggcagcc ggtttgattg ggagccttgg caacgactcg acgcgacact ggattcctta
     2041 cccggactac agtccgggtt gattgtcgcc cccgccgaac tcaaaatact tcctcaaaaa
     2101 gaggttctat tgagcgacgg ccgggtggtg gatcttgatc gcaatcaaat cagcggcctg
     2161 gatccggggg attggtgcat cgtttttgcc gcaactcccg gggagaaatg gtacggggtg
     2221 cttacccggc agggaacagg ttgggtcccg gaaaaggcgg tggctctctg ctccaaaacc
     2281 gaggtcgact cgtactggaa atttcagccg cgcttgatgg ttctcgatcc ggcggccaag
     2341 gtctttacga atgggggcac actgccgatc agcatgggga gcagcctgcc ggtgaagaat
     2401 tttgccgata gtttagtggt cctgcctcag cggggttccg atggaaagct gcgtttagag
     2461 accgtaatgc tcaaggggca attctgtatg gagaaacttc ctgccacccg ccataacctg
     2521 attcgtcagg cttttcaata cttgggttac cgttacgcct ggggtgactt cgattgtgag
     2581 gggcggggcc gggattgctc ccgggtggtt caggacgctt tttctaccct ggggttgaag
     2641 ctgccgcggt attcccagga acaattggct gcagggctca accggatcga tctggcaggc
     2701 cggggagagt tcgagcgccg cgaattactg gattgttgtg gtttgggtga tttgcttttt
     2761 accaatagtc atgtgctaat ctttctgggt aaagaagcgg ggagatacta tgccctgcat
     2821 gctttctata aatatcaagc cccggttccc atgggggagg ttaccaagac cgtcaagcaa
     2881 gtggtggtct ccgacctttc gcccggggaa ggaacgtcta ccggttcctt gttgcaacgg
     2941 ctcaccgggg tggtctcact cttaacttga gtgtactcac tcataacatg aggtgcaccc
     3001 actcttaaca tgagtacact cacttcgtga ataaactcat ggggtgagta gactaacgga
     3061 gtgtgagtgg actcacagtc tcgaaagatc ttaatcaaaa agtccgggat acaggagtgc
     3121 gtgcgtgatg gaacagattc tggtgaccgc cgccgtcata gccgagccgg ggcgggggat
     3181 tcttttggcg cagagattac cttacggccc ggaaggaggc aagtgggagt ttccgggcgg
     3241 aaaactcgaa cccggggagt caccccaatc ctgcctaagc cgtgaactcc gtgaagagtt
     3301 gggagtggcg gcaactgtcg gtccggtttt ggacattgtc tctcaaaacc gggagaaccg
     3361 gcagttgatc ttagcatatt ttctttgccg gattgattcg ggagttcccg ccgctcttga
     3421 aagtcaggcg gtgaaatggt ttgacccgca gcaaattctc tcgctcgatt tgtccggggc
     3481 ggatcgggag ttttgggaga atactcgccg aaaccggcag gaatgggtta ccgctttaac
     3541 ggtgattggt cccgggtcat ccggccggtt cggatcagtt cctcgatctt gaaagtgccg
     3601 catttacggg cattaagaaa cccatcccag gctgcttggg caaattggcc gtcatttagc
     3661 aagtcaaaga gccattcggc ggtgagcgcc ggatcctctt gcgcgatacc ggctctatgc
     3721 tcaatcagcc attttttgtt aaaatgttca tgtgcgccga gcggtggggc gatgataatc
     3781 ggcaaaccta acccacaata aaacgaaagc tcggagggct tggtccataa aatatccgtg
     3841 gtccgcaaca tcaggttgaa gcgttgaaag tatttctccc ggttcgggct gtacaaaata
     3901 ttcaacccgg taccgagttc gccgtcgaga cctaacgcga cggcttcctt gtgataatag
     3961 tcgtggatct cctggcgcac cccggcaacc agattcagac ggatccggcg gttcaagatg
     4021 ctggcacgga ggcttttcaa gatctcacag ccaatctctt tttgtgctcc ggcaccgcct
     4081 accgcgaagg tcagggtaag tatccggtcc ccccgcttgg tgcagttctc ggcgcccaga
     4141 taatactcgg cttccacgcg gtggagagtc cagaaccgtt ctccggggtc caaatagatt
     4201 aagcgctgcg ccaggtccag cttgaggatt tcaagatcag gcccgcccaa att
//
LOCUS       DASVMB010000388         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_48702,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000388 DASVMB010000000
VERSION     DASVMB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48702"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF531_10980"
     CDS             complement(<1..180)
                     /locus_tag="VF531_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7714531.1"
                     /translation="MDIRNCRRCGKLFSSTGSPVCSGCTRQDQEDFDKVRDFLFDHPN
                     STILEIAKATEVDGKT"
     gene            complement(184..981)
                     /locus_tag="VF531_10985"
     CDS             complement(184..981)
                     /locus_tag="VF531_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7714532.1"
                     /translation="MNKLKKEFSVIRQIGLGFRELFFPDRELCPVCRREPSEGGGLGI
                     QCFSRISLITEPICQKCGRPLRLKSATAKLCEQCSTSRYYFSQARAVALYEGALRENL
                     TDLKYRYRPDLGWALGLLLVEWVKLHRDFQTFDFIIPIPIHRQKLALRGYNQAELLAN
                     PLQRYLGIAIKNDIIIRDKITESQSVLDRSHRLANINEAFRVADTRDLAGKRILLVDD
                     ILTTGATASEASRVLLRGGALDVKVLTLAAGVIDSQWFIDSGINCGG"
     gene            complement(1105..2895)
                     /locus_tag="VF531_10990"
     CDS             complement(1105..2895)
                     /locus_tag="VF531_10990"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7714533.1"
                     /translation="MTDKWSEGVLLLAPQYYMYLAVDSAGTYRPGISSNPRLEAYLLD
                     GLGYIEIYLVQPPQPIWVAELFLAALKKVNHFRSPRHFIHWVKGSITGFSPKLLLKLV
                     RYSLSELKLMDYEAPEWGEGLSGYNLLLEEIPRFVHSGGYQLGWDPETDLQYLVFHSQ
                     IRREAAVGLDLLGMPFCRRCGATSGIIADDCIHCGSRDCYICMNCKTLGISRSCQPLY
                     SRSGVPMVTIKNSTRDKSVTVKPEMEFELTQAQTRASRQLLDFLDGPDSAFLVWAVCG
                     GGKTEVSFAVVARVLSAGGRVLFAIPRKDVVVELKPRFEKAFPGIAVTALYGGSGGKF
                     QDVPLTLATTHQCLRFFERFDLVILDEADAFPYQDSAMLHYAVERARKPSGKLVIMTA
                     TPDKTLLRKVKAGGLPFVTIPARPHRKPLVVPEVFRAGIPNPVSFGEQWTPPQFMVDF
                     VVGLRQTGRKGLIFFPTIGLIERWGPPVIRWAKGNCLQGAVTSGPKGNASVVKQDLRD
                     GKLDFIVCSTILERGITIPNLDILVVAADYETVFDSRTLIQISGRAGRLGDSARVLFV
                     GSTVSRSMREAVEQIRELNEEGLRLGYLDP"
     gene            complement(3045..5222)
                     /locus_tag="VF531_10995"
     CDS             complement(3045..5222)
                     /locus_tag="VF531_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX7714534.1"
                     /translation="MDQLAGTVDRVTFYNEDNFYTVAKFKTEQDHKLVTVVGNLPPLY
                     AGEELLLKGEWVTHKEYGQQFQIVEWESIEPQTLLGIERFLGSGLLKGVGPATAKKIV
                     KHFGTRTLEVIRNSPEEMIVIPGFSSVKADRIADSLREQGAIERIMVFLQGVGVSPGY
                     ALKIFQTYQDQAIQIVKENPYRLADDVFGIGFKVADDIARKMGIAIDSPYRIQAGIRY
                     LLNENSENGHIYALEKEFFARAQAELSLNELQLAAELEQLIVKKDIFREVRADGERLL
                     YLALFYYSELGVASRVRELAQTGAPVLHVNVESLLEEFSQTNQISLADNQREAIEKAL
                     ESSLLIITGGPGTGKTTIIKAILHLLKAAKLRVMLAAPTGRAAKRLAETTGEDASTIH
                     RLLGYGNEEFGGSRFQYNEDEPLETEALIIDEFSMVDLLLFYHLLKAIKPGTRLILVG
                     DVDQLPSVGPGSVLRDLIGSGRLSVVRLNVIFRQAKESLIITNAHRINKGEYPIISKD
                     QDFFFIEENDPERIVQVIPELVKTRLSRYLNCDPVEDIQVLSPMRRTITGVVNLNLCL
                     QEALNQATPEKPELKTGSLLFRLGDKVMQIKNDYQRQVFNGDIGRIRQLDAEDRRLVV
                     GFPEVGGERSVVYESENLDQLVLSYAISVHKSQGNEYPVVVMPITTQHFLMLQRNLFY
                     TAITRARRLVVLVGTKKAIVLAVKNNKIEERHSLLKERLGQIM"
     gene            complement(5263..>5570)
                     /locus_tag="VF531_11000"
     CDS             complement(5263..>5570)
                     /locus_tag="VF531_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX7714535.1"
                     /translation="LEIVALFDIDENKIGRKIADVDIYHLSQLPRIIKEQDIRMMILA
                     FPAENVQEVVDTCIACGIRSFLNFIPATIKLPPGVKMHTSDVTLQLQSLAYYTSNDQ"
BASE COUNT         1469 a         1525 c         1251 g         1325 t
ORIGIN      
        1 ggttttacca tccacctcgg tggccttggc tatctccaga atggttgaat tcgggtgatc
       61 aaacagaaaa tcacgaacct tatcgaaatc ctcctgatct tgacgagtac aaccgctgca
      121 aaccggcgaa ccggtggaag aaaaaagctt gccgcagcgt ctacagttac gaatatccat
      181 tttttatcct ccgcaattaa ttccagaatc aatgaaccat tgcgagtcaa taacaccggc
      241 tgccaatgta agaaccttga catccagggc gcccccccgg agcagaaccc gggacgcctc
      301 ggaagcggtg gctccggtag ttagaatgtc gtcgaccagt aaaattcttt taccggcaag
      361 atctctcgtg tccgccaccc ggaaggcctc attaatattg gcaagacgat gggaacgatc
      421 taaaacgctt tgagattccg taattttgtc cctgattatt atatcgtttt ttattgcaat
      481 ccccaaatat ctctgcaagg gattagctag caattccgcc tgattataac cccgcagggc
      541 caatttttga cggtgaatcg gaatcgggat gataaaatca aaagtttgaa aatcccgatg
      601 aagcttaacc cattccacaa gtaacaatcc caaggcccat ccgagatccg gacgatatcg
      661 gtactttaaa tccgtcaaat tttcacgaag cgctccttcg tacaacgcca ccgcccgggc
      721 ctgcgaaaaa taataccggc tggtgctaca ctgttcacaa agtttcgccg tagcgctctt
      781 caaccgcaac ggccgaccgc atttctgaca aatgggttcg gtaattagcg agattcgact
      841 gaaacattga attcccaagc cgcccccctc actcggttcc cggcggcaga ccgggcacaa
      901 ctcccgatcc ggaaagaata attccctgaa gcccaaacca atttggcgaa tcacgctgaa
      961 ttcctttttc aatttattca tcgtcaaatt caccggtttc tttaataaga ccaaagtcct
     1021 aattttggaa gaaatacgtc caaaccattg agttatcgta aatgccttaa tatgttaact
     1081 tgattttaac ctcaatagat agctctatgg gtcgagataa cccaaacgca ggccttcctc
     1141 attcaactcc cggatttgtt caaccgcctc ccgcatcgac cgggaaacgg tggagccgac
     1201 aaaaagcacc cgggccgaat cacctaatcg gcctgcccgg ccggagatct gaatcaaggt
     1261 gcggctatcg aatactgttt cataatccgc ggcaactacc aatatgtcca gattggggat
     1321 ggtgatgccc cgttccagga tagtggaaca tacaataaag tccaatttcc catcgcgtaa
     1381 atcctgcttc actacactgg cattgccctt ggggcctgaa gttacggctc cttgtaagca
     1441 attccccttc gcccaacgga tcaccggcgg cccccaacgc tcgatcaacc caatcgtagg
     1501 aaagaagatc aaccctttcc ggcctgtctg ccgaagccct accacaaaat caaccataaa
     1561 ttggggggga gtccattgtt ccccgaaaga gaccgggttg ggaattccgg cccggaaaac
     1621 ttccggaacc accaaaggct tcctgtgcgg tcgggctgga atcgtcacaa aaggtaaccc
     1681 acctgctttg actttacgaa gtaaagtttt atccggggta gcggtcatga tcaccagctt
     1741 acccgagggc ttgcgggcgc gttccactgc gtaatgaagc atggcgctat cctgataggg
     1801 aaatgcatcc gcctcatcca gaatgaccag atcaaaacgt tcgaaaaacc gcaagcattg
     1861 gtgcgttgtg gccaaagtta agggaacatc ttgaaatttg ccaccactgc cgccgtataa
     1921 tgcggtgacc gcgataccag ggaacgcctt ctcgaaccgg ggttttagtt ccaccacaac
     1981 atccttgcgg ggaatggcga aaagcacccg gccaccggca gacaaaaccc gggcaacaac
     2041 tgcgaaactc acctcggtct tacctccgcc gcatactgcc cagaccagaa aagcggagtc
     2101 cggtccgtcc aggaaatcaa gaagttggcg ggacgcccgg gtttgggcct gagtcagttc
     2161 gaactccatt tccggtttga ccgttaccga tttatcccga gttgagttct tgatcgtaac
     2221 catcggtacc ccggatcggg aatacaatgg ctggcagctc cgggatattc ccaacgtttt
     2281 gcaattcatg cagatataac agtcccggct accgcaatga atacaatcat ccgcaatgat
     2341 tccactggtg gctccgcaac gccggcaaaa cggcattccc agtagatcca accctaccgc
     2401 cgcctcacgc cggatttgag aatggaaaac cagatattgg agatctgttt caggatccca
     2461 gcccaattgg taaccacccg aatggacgaa gcggggaatc tcctcaagta acagattgta
     2521 accggataga ccttcccccc attccggtgc ttcataatcc atcaacttta gctcagagag
     2581 cgaataccta accagtttca aaagaagttt gggcgaaaaa cccgtaatag atcctttgac
     2641 ccaatggatg aagtgtctcg gggaacgaaa atgatttacc tttttgagag ccgcgaggaa
     2701 tagctcggca acccagatcg gctgcggcgg ttgtacaaga taaatctcaa tataacccag
     2761 accatccaga agataggcct ccaaccgcgg gttgctgctg atacccggcc ggtatgtccc
     2821 ggccgaatct actgccaaat acatgtaata ctgaggtgcg agcaataaaa ccccctcgct
     2881 ccatttatcg gtcatctgaa ttaatcataa ttctccgaat tgtataacaa ttcctgccgg
     2941 aaatatggac aaaagtatag gtatcataaa ataaacgcga atggagtgag gaagaatctt
     3001 tggaggtaaa acgggaaacg aagtttgtaa gatcttaggg gatactacat aatttggccc
     3061 aaccgttcct tgagcaagga gtggcgttcc tcaatcttgt tatttttcac cgcgaggacg
     3121 attgctttct tggttccaac cagtaccacc agtctccgag cccgggttat cgcggtataa
     3181 aaaagattcc gctgcagcat caaaaaatgc tgggtggtaa taggcattac aaccaccggg
     3241 tattcattgc cctgactctt atggaccgag atggcatatg agagtaccaa ctgatcaaga
     3301 ttttcggatt cataaacaac ggatcgttcc cctcccactt cggggaatcc caccaccaac
     3361 cggcgatcct ccgcgtccaa ctgccggatc cggccgatat ccccattgaa gacctgtctt
     3421 tgataatcgt tcttgatctg catcacctta tcacccagac gaaacaggag tgaaccggtc
     3481 tttaactccg gtttctcagg ggttgcctga ttcagcgctt cctgcaggca aaggttgaga
     3541 ttaaccaccc cggtgatggt gcggcgcatc ggggacaaca cctggatatc ctctaccgga
     3601 tcgcaattca gatagcggga tagccgcgtc ttaaccaatt cgggtataac ttggacaatc
     3661 cgttccgggt cattctcctc aatgaagaag aaatcctggt cctttgagat tatgggatac
     3721 tcacccttat tgatacggtg agcgttggta atgatcaaac tctccttggc ttgccggaaa
     3781 atgacattca gccggactac cgaaagcctc ccggaaccaa ttagatcccg cagcaccgat
     3841 cccggtccca ccgagggaag ttggtccaca tcaccgacca ggatcagccg ggtgcccggc
     3901 ttgatcgcct taagcagatg atagaagagt aatagatcga ccatggaaaa ttcatcaatg
     3961 atcaaagcct cggtctcgag gggctcatcc tcgttatact ggaaccggct accgccgaac
     4021 tcctcgttgc catagccgag caaacgatgg atcgtactgg cgtcctctcc ggtagtctcc
     4081 gccaacctct tggctgcccg gccggtgggt gcggccagca ttacacggag tttggccgct
     4141 tttaacaaat gcagaatggc cttgataatc gtggtctttc ctgtccccgg gccgccggtg
     4201 atgatcaaca ggctactctc gagcgccttc tcaatggcct ctctctgatt atcagccaag
     4261 gatatctgat tcgtttgcga aaattcttcg agcagcgact caacatttac atgcagaacc
     4321 ggagcgccgg tttgcgccag ttccctgaca cgggaggcca ctcccagctc actatagtaa
     4381 aataacgcca ggtaaagcag tcgttcgcca tcggcccgga cttccctgaa gatatccttc
     4441 ttaacgatca gttgctccaa ttcagctgct agttggagtt cattcaaact taattcggct
     4501 tgggctctgg caaaaaactc cttttcaagc gcatagatat gaccattttc actattctca
     4561 tttaacagat agcgaattcc ggcctgaatc cggtacggcg aatcaatagc aatccccatt
     4621 tttcgcgcaa tgtcatccgc cactttaaaa ccgatcccga aaacatcatc tgccaggcgg
     4681 taaggatttt ctttaacgat ttgaatggct tggtcctgat aagtttggaa aatcttcaac
     4741 gcatatcccg gactgacccc gactccctgg aggaaaacca tgattctttc gatagcccct
     4801 tgttccctca agctatcagc gatccgatcc gctttcaccg agctgaaccc ggggatcacg
     4861 atcatctcct ccggcgagtt tcggatgacc tcaagagtcc tggttccaaa atgtttaacg
     4921 atctttttgg ctgttgccgg accaacaccc tttaataaac cgctacccaa gaagcgttcg
     4981 attccaagca gtgtctgagg ttcaatgctt tcccactcca cgatttgaaa ctgttggccg
     5041 tattccttat gggtaaccca ttcgcctttc aggagcaatt cctctccggc ataaagcggc
     5101 ggcagattcc ccacaaccgt aacgagcttg tgatcttgct ctgttttgaa tttggcgacc
     5161 gtgtaaaagt tatcttcatt ataaaaggta acccgatcca cggttccagc caactgatcc
     5221 atctccaggc ctcccgaaac catcaatgat catgaccaat aatcattgat cattgctcgt
     5281 gtaatacgcc aaactttgca gttgcaaggt aacatccgag gtatgcatct tgaccccggg
     5341 cggtagttta atcgtggccg gaataaaatt cagaaaggaa cgaataccgc aagcgataca
     5401 ggtatccacc acttcctgaa cattttcggc ggggaaggcc aggatcatca ttcgaatatc
     5461 ctgttccttg atgatccggg gcaattggga cagatgataa atatcgacat cggcgatctt
     5521 tcggccgatc ttgttctcgt cgatatcgaa taaagccaca atctccaagg
//
LOCUS       DASVMB010000389         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_712567,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000389 DASVMB010000000
VERSION     DASVMB010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712567"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VF531_11005"
     CDS             <1..1143
                     /locus_tag="VF531_11005"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7714536.1"
                     /translation="KIAIEARPIKWSYGTGIGNYTYCLIQKLAEVDRGNDYAFLWPDT
                     GDTAELPFPRSHGIYNLPKDDEREEFEIPLWLAKERVDLFHLPQNGFRIPQAKTCKIV
                     VTIHDLIPYFLPEMVRPSFLKRFTREMPYIVERADQIVTVSEASKRDIISVFGIEPAK
                     ITVLPSAPSAAYRPLSPEATRRRLASTYGIKRRFILYVGGLNPRKNVAELIQAYAKIN
                     RELRDGQQLLILGPECKHRFRLQLLAEALGLKDRVIFPGFVANDDLPLFYNGADLFVY
                     PSLYEGFGLPPIEAMACGTPVITSNVSSLPEVVGQAALQFAPDDTLQLAESIFRVLDD
                     LTLRRLLITKGLERSANYSWDRIARQMLNLYQTVVNDVSSPTAVGQ"
     gene            complement(1106..1990)
                     /locus_tag="VF531_11010"
     CDS             complement(1106..1990)
                     /locus_tag="VF531_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7714537.1"
                     /translation="MTVKGIVYAILAAFCFGIMPIFTKLLYSSTGVDPFFFLMLRYSF
                     AAILMWLFIWIRRDTSWRGLDRSSLGMITVCGVSYTIVTSTYFVALQYIDASLNSLLT
                     FTFPIFTPFLALLFFKQKLKLLPVIAALVGFAGCGLLIGGYRLRGAPHELMGIGLGLV
                     SGVIYAVYTLMGQKMTVNLKPFTITTLNLSIVACFFVTLRFGWLWRYHAPLRVYLVAL
                     LIAVVSTILANIFYFESIRNVGAIKAGIFSSFEPFFTAILATGFLGEHLSSVQWLGAA
                     FIIGGMIIVQRPWETRRR"
     gene            complement(2027..3184)
                     /locus_tag="VF531_11015"
     CDS             complement(2027..3184)
                     /locus_tag="VF531_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX7714538.1"
                     /translation="MEKTQLIATATFGLESVVAYEVKQLGYEDAKVDNARVSFSGDLG
                     AICRCNLWLRSADRVLIRVGEFQALSFEELFEGTKALPWEDWLPEDATFPVEGKSIKS
                     KLFSVPDCQAIVKKAIVERLKSRYHRQWFPENGPLYKVEVALLKDVATLTIDTTGPGL
                     HKRGYRKLSGQAPLKETLAAALIGLSYWNPDRVLLDPFCGTGTIPIEAGLIGLNIAPG
                     INRSFTAEQWLQLPEKLWQKTREEAHDLIRRDQELQISGTDIDPEVLSLARYHVGQAG
                     LEGRIHLQRLPLAELRNSHHYGCIITNPPYGERLGELPEAERLYTEMKTVFKPLETWS
                     FYILTSHPRFEKLFGRPADRKRKLYNGRIECQYYQFFGPRPPRRERTDIDD"
BASE COUNT          931 a          920 c          873 g          811 t
ORIGIN      
        1 aaaatcgcca ttgaggcccg acccatcaag tggtcttacg gaaccgggat tggcaactac
       61 acctactgcc tcattcagaa gctggcggaa gtggatcggg gcaatgatta cgcatttctt
      121 tggccggata ccggggatac ggccgaattg ccatttccca ggagtcacgg tatatacaac
      181 ttgcctaaag atgacgaacg ggaagaattt gagattcccc tatggttagc caaggaacgg
      241 gtcgaccttt ttcatttgcc gcagaatgga ttcagaatac cgcaggctaa aacctgcaaa
      301 atcgtggtaa cgattcacga tctgatcccg tatttccttc cagagatggt tcggcccagt
      361 ttcctcaagc gttttacacg cgaaatgccc tatatcgtag agcgcgccga tcaaatcgtc
      421 actgtttcgg aggcttccaa acgggacatc atttctgttt ttgggattga acccgccaag
      481 atcacggtac tcccctctgc accgtctgcc gcgtaccggc cgttatctcc ggaagccacc
      541 cggcgcaggc tggcttcgac ctacggcatc aaacgaaggt tcatattata tgtaggaggg
      601 ctcaaccccc ggaaaaatgt ggctgaatta atccaagcct atgccaaaat caaccgggaa
      661 ctccgtgatg gccagcaact tttaatcctg ggaccggagt gcaaacaccg ctttcgttta
      721 caacttttag cggaagccct gggactgaag gaccgggtaa tctttcccgg attcgtggcc
      781 aatgacgatc tgccgctctt ttacaatgga gccgatctat tcgtctatcc ctcattgtat
      841 gaaggattcg gcttaccgcc gattgaagcc atggcctgcg gcacaccggt gatcacttcc
      901 aatgtctcct ccctcccgga ggtggtaggt caagccgcat tgcagttcgc tccggatgat
      961 accttgcagc tggccgagag catcttccgg gtacttgatg atttaaccct gcgccgcttg
     1021 ttgataacca aggggttgga acgaagcgcc aattacagtt gggaccggat agcccgccag
     1081 atgttgaatc tttaccagac agtggttaac gacgtctcgt ctcccacggc cgttggacaa
     1141 taatcatccc gccgataata aaggcagccc ccaaccattg aaccgaagat agatgttctc
     1201 ccaaaaagcc ggtagccagg atggcggtaa agaaaggctc gaaactactg aagatccccg
     1261 ccttgattgc acccacgttg cggatggatt caaagtaaaa aatgttggcc agaatggttg
     1321 aaactaccgc tatcaataag gccaccaggt aaacccggag cggagcatga taccgccaca
     1381 gccatccgaa acggagcgtt acaaaaaagc atgccactat cgaaagatta agtgtggtaa
     1441 tggtgaacgg tttcaaattg acagtcatct tttgacccat gagtgtatat acggcataga
     1501 tgacgccgga gacgagtccc aaaccgatgc ccatcagctc atgaggtgcg cccctcaaac
     1561 gatatcctcc gatcaaaaga ccgcaacccg caaagccaac caacgccgca atgaccggca
     1621 acagtttcag cttttgtttg aagaataaaa gggccagaaa tggcgtgaat attggaaagg
     1681 tgaaggtcaa taaggaattg agtgaggcgt cgatatattg taatgccaca aagtaggtgc
     1741 tggtgacgat ggtgtaactc acaccgcaaa cggtgatcat tcccaggctc gaacggtcca
     1801 gcccccgcca gctggtatcc cgcctgatcc aaatgaataa ccacatcagt atcgccgcaa
     1861 aagagtagcg aagcatcaaa aaaaagaacg ggtccacccc ggtcgaactg tatagcagct
     1921 tggtaaagat gggcatgatt ccaaaacaaa aagcggccaa gatggcataa acaatacctt
     1981 ttacagtcat tacggcacct ctcaacgaat aacaaataac caataactaa tcatcaatat
     2041 ccgttctttc ccgccggggc ggccggggac cgaagaattg ataatactgg cattcaattc
     2101 tgccgttgta cagcttgcgt ttccggtcgg caggccgtcc aaatagtttc tcaaagcgcg
     2161 ggtgggaggt aagaatataa aatgaccagg tttccagggg tttaaaaacc gtcttcatct
     2221 ccgtatataa tcgttccgcc tccggtaatt cgccgagacg ctccccatag ggcggattgg
     2281 ttatgataca gccataatga tggctatttc tgagttcggc aagggggaga cgttgcaaat
     2341 ggatccgtcc ttccagtccg gcctgcccaa cgtggtaacg ggccaggctc aggacctcgg
     2401 gatcgatatc agtccccgag atttgcagtt cttgatcacg acggataaga tcgtgagcct
     2461 cttcacgggt cttttgccaa agtttctccg gaagctgcag ccattgttcg gcggtgaaac
     2521 tccggttgat accgggagca atattcaagc cgattaaacc ggcttcgata ggtatggttc
     2581 cggtaccaca gaaaggatcg agcaacaccc gatcgggatt ccagtaactc aagccaatca
     2641 aagccgcggc cagcgtctcc ttgagtggtg cctgccccga tagtttacgg tacccccttt
     2701 tatggaggcc gggtcctgtg gtgtcaatgg tcaaagtggc gacatctttt aataaagcga
     2761 cttccacttt atagaggggg ccgttttcag ggaaccactg ccggtggtag cgactcttca
     2821 accgctcgac aatggctttt ttgacgattg cctgacagtc ggggacgcta aaaagctttg
     2881 attttatgga tttaccctcc accggaaaag tggcgtcctc cggaagccaa tcctcccagg
     2941 gcaaggcctt ggttccttca aagagctcct caaaagagag agcctggaat tcacctacgc
     3001 ggattaaaac ccggtcggcg ctccgtaacc ataaattgca gcggcagatc gcgccaaggt
     3061 cgccactgaa actaaccctg gcgttgtcta ctttggcatc ctcgtaaccc agctgcttta
     3121 cctcatatgc cactacggac tccaaaccaa aagtggcggt ggcgatcagt tgcgttttct
     3181 ccaagttcgt cctctccgat attcattttg tcttctgcat ttcgtttttt agtttgtccg
     3241 taagccgtga agccgactca ccctagttat ctccccttat acggtaagcg ggaaggaggg
     3301 gaaaaggcgg actcaaagca gtgataaaat agactgcgaa gggtagggtt actctctcta
     3361 gcgcccgtcg ctagagagag caaaagagag tcgtaggcaa tgaagccgac tcaccccact
     3421 agctcccctt atacggtaaa cttagattaa gatattcctc aaccgtaact gagtaggggt
     3481 cggggaagat ttggcagaat aataagtttc acctatgttc atcagaagca aaact
//
LOCUS       DASVMB010000390         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_416935,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000390 DASVMB010000000
VERSION     DASVMB010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416935"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VF531_11020"
     CDS             complement(<1..362)
                     /locus_tag="VF531_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7714539.1"
                     /translation="MNTGKRLMHYVKPYWRQLTVGFVTMVFMSAIQLVYPVIMGPGLI
                     DRVLNTKDFRMLNWLVLLVIVAVSIKACLTFAQNYFMAFVGQRVVANLRNAVFHHLQK
                     MSIAFHESHRVGEIISRVT"
     gene            complement(398..1690)
                     /locus_tag="VF531_11025"
     CDS             complement(398..1690)
                     /locus_tag="VF531_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714540.1"
                     /translation="MPQTFSIVITVNSPGEVSGWLKPTVKAIREWNRPVTITVFIPPC
                     TFASGTEKNVVQAIPGIDLVVTSSELFRYIVLGRKPKGFHPSPQGIVLFLGGDLTYAA
                     LLAKRLKYPALAYSEGVVNWTNIFRYFATPYPKNARKLAAKGAPASQIRTIGNLMLDA
                     IPTDLDGKKIREELKIGDKPVLLLMPSSRPEHFEYMLPFLIQVSGEIHKQIPGLYPVI
                     SISPFITDRQLAGVLTGEYAGVLGVTGSYKDGQTVHGVTSWEQPGSFLTSDGTVISAF
                     RGRQYELMAAADLAISLPGTNTFELTAFGVPTIVTLPLTHPERIPIEGPIGLLGKIPG
                     FGGWLKRTLLPRMALKIKYTSWPNRLAEAMITPEVRGLITAGDVSAVAVSLLADPEER
                     KRISGELRKLVGERGAAPKLVQLMEIIISEHYGGFESQ"
     gene            1964..2971
                     /locus_tag="VF531_11030"
     CDS             1964..2971
                     /locus_tag="VF531_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714541.1"
                     /translation="MSLMIAMLMMIIWVFTLLIAIFGIINLWNTVRFQTYRTRLASPI
                     PPLPVIKNFAIKNPYAAPGYYYKAQLHLHTSNSKDVHPKLPVAETVRRYQAAGYQVLV
                     ITDHDTTTRYVPPDFPDLLVLAGNEKTIPQPFWPLGKHLVQIEYFHSQNRLGMPAHPN
                     WQGNLGTGCWYLGDLLKLPDFHLMEIYNHHSDYRDDLLLWNQLLRHFGPEHPVWGVAV
                     DDSDNAELLDRGWIMVKAEHLTEDTFIKALRQGSFYATTGPVLEFQVNANKLIQVSTP
                     VAGTIRFLNHQGKVMGQAEGTLGEYRPRGDEGFIRVELEVAGRFAWSQPFFIIPIHEH
                     L"
     gene            3032..3946
                     /locus_tag="VF531_11035"
     CDS             3032..3946
                     /locus_tag="VF531_11035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7714542.1"
                     /translation="MKPVKIAAVVVTYKTKALLENCLKSLYTEMASLGCSCLVTVVDN
                     ASNDGTAEMVREKFPEALLQINTENLGPARGFNRGISGIIEKAENILVLNSDIEILPG
                     TLRAMLEVLENEPDVDGVSGPLLNEDHNRQMIKTHIWRLRPVDFEKRFRIEFVGTTFA
                     LIRAATFHKIGGYDENYYFHNEDLDWAVHAKREGYRFMYLPDAPVIHFQSKGKIQNQS
                     RIIRETYCSDIYFYKKFYPRWAFLAYGASNLNLDMLIRSHRSKLKRETNPEKQAELSR
                     ILADYIEAKERMKQEYHTNRPPKVPQWN"
     gene            3992..4786
                     /locus_tag="VF531_11040"
     CDS             3992..4786
                     /locus_tag="VF531_11040"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEX7714543.1"
                     /translation="MEPQKVAFVICINDETLYSRCRNYLDQLIIPPGFKVEVKEIRGA
                     KGMASAYNLAMRESDAKYKVYLHQDTFIFNRNLILDMVEVFTKYPKIGMIGVVGGNRL
                     PKSGIWFHDGLHSFGKVWEYRGPGSRLPLPHWNERRERIVRFRPVARPFQPVLVIDGL
                     IMMTQYDIPWREELYDSFLYYEGPQSLEYIKHGYWVAIPYQKEPWCMHWGPQQERTEE
                     GTRQMWTGIRANAEIFCKEYHDFIGVGVKRVLNRYASAGTEPKARE"
     gene            4793..>5102
                     /locus_tag="VF531_11045"
     CDS             4793..>5102
                     /locus_tag="VF531_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714544.1"
                     /translation="MQCPVCGREVGADDTVCSNCQTSLEKVQILSSEERENFQGTTIE
                     IGTSGSGRSSDDESGFYRDNNGQNQGANRSGRGRTPFPRVWILNSVLAGIIGMLFLAI
                     I"
BASE COUNT         1342 a         1218 c         1248 g         1294 t
ORIGIN      
        1 gttacccggg agataatctc gccgacccgg tgcgactcat ggaaagcaat ggacattttt
       61 tggagatggt gaaagaccgc gttacgcaga ttagccacaa ctctttggcc aacaaaggcc
      121 ataaaatagt tttgcgcgaa agtcagacat gctttgatgg aaacggccac aatgaccagg
      181 agtaccaacc aattcaacat tcggaaatct ttggtgttaa ggactctatc aattaatccc
      241 ggtcccatga tgaccgggta aacgagttga atggctgaca taaacaccat ggtgacaaaa
      301 ccaaccgtca gttgccgcca gtatggtttg acataatgca tcaatcgttt tccagtattc
      361 atcgtcgtat ccttttatag ttattggtta caagctctca ttgcgattca aatccgccgt
      421 aatgctcgct tattataatt tccataagct gaaccaattt tggggcggcg ccgcgctctc
      481 cgaccagttt ccgtaattcc ccggagattc gtttccgttc ctcggggtcc gccagcaacg
      541 aaacggccac ggcactcaca tcaccggctg tgatcaatcc gcggacctcg ggagtaatca
      601 tggcttccgc caggcggttg ggccaggaag tatatttgat ctttaaggcc atccgcggta
      661 atagcgtacg ttttaaccat ccgccaaaac cgggtatttt tccgagtaaa ccgatgggac
      721 cctcaatcgg aatcctttcc ggatgggtta acggcaaggt gacgatggtc ggaaccccga
      781 aagccgtaag ttcaaaagta ttcgttcccg gtagcgatat tgccagatcc gccgcagcca
      841 tcaactcata ctgccgtccc ctgaatgccg agatcacggt tccatcggaa gttaagaaag
      901 aaccgggttg ctcccatgaa gtaacgccat gaacggtttg tccgtcctta taactgccgg
      961 ttacaccgag aaccccggcg tattcacccg ttagtacccc ggcgagttgt cggtctgtga
     1021 taaaaggaga gatgctaatg accggatata gcccgggaat ctgtttatga atctctccgc
     1081 tcacctgaat taaaaaagga agcatatatt caaaatgctc cgggcggctg ctgggcatta
     1141 acaacagtac cggcttgtcc ccaatcttga gctcctcccg gatcttctta ccgtctaaat
     1201 ccgtaggaat cgcatccagc atcagattgc cgatggtccg gatttgagag gccggggcgc
     1261 ccttggcggc aagctttcga gcgtttttag gataaggagt tgcaaaatac ctgaaaatat
     1321 tcgtccaatt gacgacgccc tcggaatagg ccaatgccgg gtatttaagc cttttggcca
     1381 aaagggccgc atacgtcaga tcacccccca ggaataagac gatcccttga ggggaagggt
     1441 ggaacccttt gggtttcctt cccaagacga tataacgaaa aagttcggaa gatgttacca
     1501 ccaaatctat tccaggaatg gcttgaacta catttttctc ggtaccgctc gcaaaggtgc
     1561 agggcgggat gaaaaccgtt attgtcacag gccggttcca ttcacgaatc gccttaactg
     1621 tcggtttcaa ccatccgctg acttcacccg ggctattcac tgtaatgacg atgctgaatg
     1681 tttgaggcat caaacgaagg acctgccttt aacatacatt ttgagagcat tatttaatta
     1741 tattccttat tttttcgagc tgtcaaccgg aatcatccgc ttctcaccca tatatcaaat
     1801 ttataacctt gtcgttcact gccgctgtaa tccaaaaaac cactttcacg atgatgttta
     1861 gtcgtcgaaa tgtcggactt ggccaagaat tagcttgttt gcctccggtg gcaatatgat
     1921 ataataatgg cgatttatgc tgtgatatgg tgaggagcgt aaaatgtcgt tgatgatcgc
     1981 tatgttgatg atgatcattt gggttttcac tttactcatt gccatcttcg gtatcattaa
     2041 tttatggaat acagtccgtt tccagactta ccgcactcgc ttggcatcac ccattcctcc
     2101 gctgccggta ataaaaaact ttgctataaa aaacccttac gcggccccgg ggtattacta
     2161 taaggcccaa ctgcatctcc atacctccaa ttccaaagat gttcatccca aactgccggt
     2221 ggcggagacg gttcggcgct accaagccgc gggttatcaa gtattggtga tcaccgatca
     2281 tgacaccaca acccggtatg tcccgccgga cttccccgat ttactggttc tggccggaaa
     2341 tgaaaaaacc ataccccaac cattctggcc tcttggaaaa cacctggttc agattgaata
     2401 cttccattct caaaaccggc ttggaatgcc ggcccatccc aattggcaag gcaacttagg
     2461 gaccggttgc tggtatttgg gagatctcct aaagctgccg gactttcatt taatggagat
     2521 ttataaccat cattccgatt accgcgatga tttactcctc tggaatcagt tattacgcca
     2581 tttcggcccg gagcatccgg tatggggggt tgcggtggac gactcggata acgcggaatt
     2641 attggaccgg ggatggatta tggtcaaagc cgaacacttg actgaggata cctttataaa
     2701 ggcattacgg cagggtagtt tttatgccac caccgggccc gtccttgagt ttcaggtaaa
     2761 tgcaaacaag ttaatccaag tgagtacccc tgtcgcgggg acaatccgct ttctcaatca
     2821 tcaaggaaag gtgatgggac aagccgaggg taccctggga gaataccgtc ccaggggcga
     2881 tgaaggattc atccgggtgg aactggaagt agccgggcgc ttcgcctggt cgcagccgtt
     2941 cttcatcatt cccatccatg aacacctgta ggagcttgtt accggaaacc agttatgtta
     3001 aaattgatag gttacaaagg gaggattccg aatgaaaccg gttaaaattg ctgcagttgt
     3061 tgtaacatat aaaaccaaag cattactcga aaattgcttg aaatctctgt acaccgagat
     3121 ggccagtcta ggatgttctt gtttggtcac agttgtcgat aacgcttcga atgatgggac
     3181 agcggagatg gtccgggaaa aattccccga ggctttactt cagattaata ccgaaaatct
     3241 tggtcccgct cgtggtttta accgggggat ttccgggatc attgaaaagg cagagaatat
     3301 actcgttcta aacagcgata tcgaaatcct ccccggaacg ctccgggcta tgctggaagt
     3361 cttggaaaac gaaccggatg tagatggagt aagcgggccc ttactcaatg aggatcataa
     3421 ccgccaaatg attaaaaccc atatctggcg cttgcgaccg gttgattttg aaaagcggtt
     3481 ccgaatcgag tttgtcggaa ccaccttcgc cctaatccgc gccgccacgt ttcataaaat
     3541 cggtggatat gacgaaaact attatttcca taatgaggac ctggattggg cggttcatgc
     3601 caaacgggaa ggctaccgtt ttatgtacct accggacgca ccggtgatac atttccaaag
     3661 caagggaaaa attcaaaacc aatcacgcat catccgcgag acatattgca gtgatattta
     3721 tttttataag aaattttacc cccgttgggc atttttggct tacggggctt ccaacctcaa
     3781 cttggatatg ttaattcgtt ctcaccgttc caaattaaaa cgggaaacta atccggaaaa
     3841 acaggccgag ctctctcgaa ttcttgcgga ttatattgag gccaaagaac ggatgaaaca
     3901 agaatatcat accaaccgcc ctccaaaggt accgcagtgg aattgaactg tccgactaca
     3961 ggcatatgat ttaaatctta aggagcgaat catggaacct caaaaggtgg ctttcgttat
     4021 atgtatcaat gacgaaacgt tatacagccg ttgtcgcaat tatcttgatc aactgattat
     4081 ccctccgggt tttaaggttg aagtgaagga gatccggggc gccaagggca tggcctcggc
     4141 ctacaacctg gcaatgcggg aatccgacgc caaatacaaa gtctatctac atcaggatac
     4201 cttcattttt aaccgtaatc taatcctgga tatggttgag gtcttcacaa agtatccaaa
     4261 gattgggatg ataggggtgg tcggaggtaa ccgtcttccc aagagcggta tctggtttca
     4321 tgacgggctc cacagtttcg gtaaggtctg ggagtatcgc ggtccgggaa gccggttacc
     4381 tttaccccat tggaatgaac gtcgggaacg aattgtccgg tttcgaccgg tggcccgccc
     4441 cttccaaccg gtgttggtga ttgacggtct gatcatgatg actcagtatg atattccttg
     4501 gcgcgaggag ctctatgata gttttcttta ctatgagggt ccacaaagtc tggaatatat
     4561 caaacatggt tattgggtgg ccattcccta ccagaaggaa ccctggtgca tgcattgggg
     4621 cccgcaacag gagagaaccg aagaggggac ccggcaaatg tggaccggga tccgggctaa
     4681 cgccgagatt ttttgcaagg aataccacga tttcattggt gtcggagtca aacgggtttt
     4741 aaaccggtat gcctctgcgg ggactgaacc taaagcacgg gagtgaatag agatgcaatg
     4801 ccctgtttgc ggacgggaag tcggggcgga cgatacggtc tgttcgaatt gtcagacgag
     4861 tctagagaag gttcagattc tttcatccga ggaacgtgag aatttccaag ggactaccat
     4921 tgaaatcgga acatcgggat ccgggagatc ttcggatgat gagtctggat tttatcgcga
     4981 taacaatgga caaaaccaag gggccaaccg gagtggccgg ggacgaaccc cgtttccacg
     5041 cgtatggatc ctaaattcgg tcctggccgg aattatcggg atgttatttt tggcaatcat
     5101 ta
//
LOCUS       DASVMB010000391         8769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_272573,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000391 DASVMB010000000
VERSION     DASVMB010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272573"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1562)
                     /locus_tag="VF531_11050"
     CDS             complement(201..1562)
                     /locus_tag="VF531_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714545.1"
                     /translation="MRRKLQKTIGLVMPVLLVVLLAGQIFAAPKPVTIRFMYWADGEQ
                     KKVVEQVCKKFNQLNPDIIVKPEAKPSDLNFDAMMATLIGSKNLPDCSYMGETDTLRY
                     YEKGVLADLSSFFKKDLGKKLDAVTIKSTDGKTIAAGLSDQVMILYYNKDLFDKAKME
                     YPPASPDKAWDWNKFVEAATKLTVDANGKHPGEAGFDPNQVVTYGVNVSFFFQFSFMW
                     ALNSNGGGTVSPNGKELWFNKPESIDALQKVADLINKDQVHPAVGSNQAGAIGGVDQA
                     LLSGKVAMFISGSWDIANAGRAKRENSLNYGIAALPKLKVPVTMNCGAPLVIYKTSKH
                     LAQAKRFYKFMMDPEQVLDIIKTGAWLPNEEKWYTDPALLAKWIDSPNFPSEARTLIP
                     AYALKYTAQWPAYSCPPWSRMGSQFDPAWDALWAGKKTAKETVDSFMPKIKPIFASGK
                     AND"
     gene            complement(1841..2884)
                     /locus_tag="VF531_11055"
     CDS             complement(1841..2884)
                     /locus_tag="VF531_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714546.1"
                     /translation="MTTIKEIAKLANVSTATVSYTLNDPSKISEETRNKVLQVINRVN
                     YKPNSIAKSLKVKKTNTIGIITEDIATFIAPEIINGIDEYAEQKKYHILLNNIRLHKR
                     LGIHFDDTVKYSRIISEITSVLLSKQVDGFIYLGVYCRDVTGLIDKIPVPIVYTYCFT
                     QNENDVSIGYDDELAAYEIVQYLVSKGHRKIGVITGLMDSVHAQDRLKGYQRALYEHN
                     ILFNPQYMVIGDWNERGYTAGKELLTLPDRPTAIFAMNDIMAGGVIDAANEIGIKIPG
                     ELSLVGFDNRECSTAYTPRLTTMAIPLNEMGQLSAETLITMINGDYDESHPKRIIKLP
                     CKLVERNSVSRNN"
     gene            complement(3062..4405)
                     /gene="dnaB"
                     /locus_tag="VF531_11060"
     CDS             complement(3062..4405)
                     /gene="dnaB"
                     /locus_tag="VF531_11060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7714547.1"
                     /translation="MSDATLDRVPPQNIEAERSTLGSMMLEKEAIYKGSEVLRPEDFY
                     REAHRVIFEVVTQLANKGEPVDIITVSEELKRREMLDKIGGVAYLTALANSVPTAANI
                     EYYARIVEEKSLLRSIISVATNIVSLGYAGSEEVDQILDEAEKQIFQISQKRNIKGFV
                     SLKSILIETFERIEQIYESKGGVTGLPTGFTDLDRMTAGLQPSDLVILAARPSMGKTT
                     FALNIARHAAVELKIPVVIFSLEMSKEQLALKLLCSEAGVDNQRIRTGTLMDNDWPRL
                     SHALGRLSDSTMFIDDTAGVSALDIRARARRIKAENGLGLIVIDYLQLMQNRGRSENR
                     QQEVSEISRSLKSLARELNVPVIALSQLSRAVEQRQVKVPSLADLRESGSLEQDADMV
                     AFLYREDYYNPETDKKNITDLIIAKQRNGPTGSIEMLFQREFSKFVGLDKYRKQA"
     gene            complement(4635..6590)
                     /gene="lonC"
                     /locus_tag="VF531_11065"
     CDS             complement(4635..6590)
                     /gene="lonC"
                     /locus_tag="VF531_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HEX7714548.1"
                     /translation="MKGIIKRVAPGVKPPVPLHERLLDDEQLVRQVAAIFALLSNLYG
                     SDKLVLKAGKLEALKLMRSEVLEERVLALQKIVFEDPTIDTLPHRKDRPQVLNEIEEE
                     IADLIARRTVEEKIEKKIADRMQQRHEEYLKEIKIQILQESAGPDNPHTLKKLAELEK
                     LDSINLSKSALETLKPTKLNEVVGQDRAVKALLSKIASPFPQHVILYGPPGVGKTTVA
                     RLVLEEAKRIPFTPFKEIAPFVEVDGTTLRWDPREVTNPLLGSVHDPIYQGAKRDLAE
                     GGVPEPKLGLVTEAHGGVLFLDEIGEMDPMLLNKLLKVLEDKRVSFDSAYYDPNDPNV
                     PKYIKKLFSEGAPADFILIAATTRDPSEINPALRSRCAEVFFEPLDQQDIQIIVTNAA
                     ERLEVRIAPEVPELIAEYTIEGRKAAGILADAYGLALYKNKDQQPDRGEITIGQEDLL
                     EVFQVSRLSPYLTVRGSERTECGRIFGLGVYGFVGSVLEIEAVAFAASDKGKGRLRFN
                     ETAGSMTKDSVFNAASVVRLVAGMDLSDFDVHVNVVGGGKIDGPSAGVAITLAIISSL
                     TKQAIPQNIAVTGEVSIQGKIKAVGGVFEKIYGARQAGMKKVFIPKENEKDVPTDLKD
                     IQIIPVETIQEIIADALIPAENLATAN"
     gene            complement(6862..7308)
                     /gene="rplI"
                     /locus_tag="VF531_11070"
     CDS             complement(6862..7308)
                     /gene="rplI"
                     /locus_tag="VF531_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7714549.1"
                     /translation="MKLILKQDVKALGRKGDVKQVADGYAQNFLLPKGLAVEATPANM
                     KLLEDQKQSAIRRELLDEAEAKEMAVKLNGLALHFKVKAGEGGRLFGSVTAKDIADQI
                     QKMIHFELDKRKLELDEAIKAVGDHPIKIHLYKGVTATIHVQVSAE"
     gene            complement(7322..7657)
                     /locus_tag="VF531_11075"
     CDS             complement(7322..7657)
                     /locus_tag="VF531_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEX7714550.1"
                     /translation="MSSNNPDFNIVKNVRIIEWLKAEIVAGLGSLFKAMVKNSEEAIQ
                     DSLAWLIVSCYFLGKRLGIPFAKLDARIEQQLQAPQLQDHEIEEWYGDVTNLQNYLRE
                     RNLKEPNRM"
     gene            complement(7890..8150)
                     /gene="rpsR"
                     /locus_tag="VF531_11080"
     CDS             complement(7890..8150)
                     /gene="rpsR"
                     /locus_tag="VF531_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7714551.1"
                     /translation="MPRNERERGDREPGRGRRPKKKVCSFCVDKVEHIDYKETAKLRK
                     FITERGKILPRRISGNCAIHQRQLTSAIKRARQIALLPFTSE"
     gene            complement(8250..8711)
                     /gene="ssb"
                     /locus_tag="VF531_11085"
     CDS             complement(8250..8711)
                     /gene="ssb"
                     /locus_tag="VF531_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7714552.1"
                     /translation="MNHIVLIGRLTRDPELRYTPNGVAVANFDLAVDRPTPNQQGERE
                     ADFIRIVAWQKQAELCANYLKKGRLVGVEGRLQIRNYETQDGQKRKAAEVIASFVQFL
                     DKGRDDAGAPSTGSGFTPGTGSSGGRTNAGGDPGSDPAEDLGGINLDDLPF"
BASE COUNT         2094 a         2343 c         1994 g         2338 t
ORIGIN      
        1 aaaaatgagg aacccggcga tcgcaggaag ggtaaagaga taacccgcta tcgcctcttg
       61 gggtttgtat ttttttatcc gcgccggatt tcgccccaaa tcttttttta aagccgtttc
      121 ccccataaat gtcacctctg taatggtccc gccaatgcgc gcaacctatt gcgagattgc
      181 gcgtattggc gagctttccc ttaatcgttc gccttcccgc tggcaaagat cggcttaatc
      241 ttgggcataa aggaatcaac cgtttccttg gcggttttct ttccagccca caacgcatcc
      301 caggcgggat caaattggga acccattctt gaccagggag ggcaactgta ggcaggccac
      361 tgcgccgtat atttcagggc atacgccggg atgagcgtcc ttgcctccga cgggaaattg
      421 gggctatcga tccatttggc aagaagcgcg gggtctgtat accatttctc ttcattcggc
      481 aaccaggcac cggtcttgat gatatccaaa acctgctccg gatccatcat gaatttatag
      541 aaccgtttgg cctgggccag gtgcttggag gttttgtaaa ttaccagcgg cgcgccgcaa
      601 ttcatggtca ccggaacctt caatttggga agcgccgcaa tcccgtaatt gaggctgttt
      661 tcccttttgg ccctgccggc attggcaatg tcccaggaac cggagatgaa catggccacc
      721 ttacccgaga gcaatgcttg atctacgcca ccgatggctc cggcttggtt ggaaccaacc
      781 gccgggtgta cttgatcttt gttgatcagg tcggctactt tctgaagagc gtcaatactt
      841 tccggtttat tgaaccaaag ttcttttccg ttcggggaaa ctgtaccgcc gccattggaa
      901 ttcagggccc acataaatga gaattggaag aagaacgaga cattcacgcc atatgtaaca
      961 acttgattgg gatcgaaacc ggcctcgccg ggatgtttgc catttgcatc caccgtcagt
     1021 ttcgtcgcgg cctcgacaaa tttgttccag tcccaggctt tatcgggcga ggcgggcggg
     1081 tattccatct tggccttgtc aaaaagatcc ttgttgtaat atagaatcat cacctgatcc
     1141 gacagtccgg cggcaatggt tttaccatca gtggatttga tggtaactgc atccagtttc
     1201 tttcctaggt ccttcttaaa aaaggatgac agatcggcta aaacaccctt ttcataatag
     1261 cgcagcgtat ccgtttctcc catgtagctg caatcgggaa gattctttga cccgattaag
     1321 gtggccatca tggcgtcaaa attcagatcg gacggcttgg cctccggttt aacaatgatg
     1381 tccggattga gttgattaaa ctttttacaa acttgctcca caactttctt ttgttccccg
     1441 tcagcccagt acatgaagcg gattgtcacc ggtttcgggg cggcaaaaat ctggccggcc
     1501 aataaaacga caagtaacac gggcatcaca agaccaattg ttttctgaag cttccttctc
     1561 aatgataccg acctcctttt ttttactttc tatccccggg aaattattgt gtcggatatc
     1621 cctcccccaa acattattgg ttatgctaat ttaatcggtt aaacgagaag atcatgacac
     1681 ccctaaagta cttcaagcac gccttgcgtt taaaggttaa ttcttaagac tttctttata
     1741 tgtttaggta tttaatttaa tcggttaaat taaatgataa aaaaatttgg tttctctcac
     1801 gagttcataa atatccaaag gggaccgaac cgggttttta ttaattattc cgggaaacgg
     1861 agttccgttc caccaattta cacggtagtt taataatcct cttaggatga ctctcgtcgt
     1921 aatctccgtt aatcatcgtg atcaacgtct ccgcagacaa ctggcccatt tcgttgaggg
     1981 gtatcgccat tgtcgtaagc cttggagtgt atgcggtgct gcactcacga ttatcaaagc
     2041 ccaccaatga gagttccccg ggaatcttga tcccgatttc gttcgcggca tcgatgacgc
     2101 caccggccat aatatcgttc atggcgaaaa tcgccgtggg acgatccggt aaggtcaata
     2161 actctttccc ggctgtatag cccctttcat tccagtcgcc gatcaccatg tattgcgggt
     2221 taaacaagat attatgctcg tatagggctc tctgataacc cttcaaccgg tcctgggcat
     2281 gaacggaatc cattaatccc gtgatgactc cgatcttgcg atgcccttta ctcaccagat
     2341 attgaacgat ttcataggcg gcaagctcat cgtcataacc aatcgagaca tcattctcgt
     2401 tttgggtaaa acaataagta taaacgatgg gaaccggaat tttatcgatt aaaccggtaa
     2461 catcgcgaca ataaaccccc agataaataa agccgtccac ttgtttactc agcagaacac
     2521 tggtgatctc ggaaataatc ctggagtatt tgactgtatc gtcgaaatga atgccgagcc
     2581 gcttatgcaa ccggatatta ttcaatagaa tatgatactt tttctgttcg gcgtattcat
     2641 cgattccgtt aataatctcc ggggcgataa aagtggcgat gtcttcggta ataatcccga
     2701 tggtgttggt ttttttcacc ttcaagcttt tggcgatact gttaggctta taatttacac
     2761 gattaatgac ttgaagtacc ttatttcgtg tttcctcgct gatcttggaa ggatcattga
     2821 gcgtgtacga tactgtcgcg gttgaaacat tggctaattt ggcaatctct ttaattgtgg
     2881 tcatgtttat ccttataaaa atcaggttaa tcgattaaaa ccaatttcat tatacctttc
     2941 aatctccaaa ccgtctagag gataaattaa aaattattta acttttccga tatttcatta
     3001 tccagcatgg ttttcctatc aattttatta accaaataaa aaagggaacg taatgcgctc
     3061 cctacgcttg tttacgatat ttatcaagac ccacgaactt actgaactcc ctttgaaaga
     3121 gcatctcgat ggatccggta ggaccgttgc gttgcttggc gatgatcagg tcggtgatat
     3181 tcttcttgtc ggtttcggga ttataatagt cttcccggta taagaaagcg accatatctg
     3241 catcttgctc cagtgatccg gactcccgca ggtcggccag ggacggaacc tttacctgac
     3301 gctgttccac tgcccgcgat agttgcgaga gggctatcac cggcacattc aactcccggg
     3361 ccagggactt cagggagcgg gagatctccg acacctcctg ttggcggttt tcggaacgcc
     3421 cccggttctg catcagctgc aagtaatcga tgacgatcaa tcccaacccg ttttcagctt
     3481 tgatccggcg ggcccgggcc cgaatgtcca gggccgagac gccggcggta tcgtcgatga
     3541 acatggtgga atccgagagc cggcctaatg cgtgggaaag gcggggccag tcgttgtcca
     3601 tcagggtccc ggtccgaatc cgctggttat ccacacccgc ctccgagcaa aggagcttta
     3661 aagccaattg ctccttggac atctccaggc tgaaaatgac taccgggatt ttgagctcca
     3721 ccgcggcgtg acgggcgatg ttcaaggcaa aggtggtctt gcccatactg ggccgggcag
     3781 ccaagatgac cagatccgag ggttgaagcc cggcggtcat tcgatccaga tccgtgaaac
     3841 cggtgggcaa gccggttacc cctcctttag actcatagat ctgctcgatt ctttcgaagg
     3901 tttcgatcag gatgctcttt agacttacaa aacccttgat attgcgtttc tgggagatct
     3961 gaaagatctg tttctccgcc tcgtccagga tctggtctac ttcctcactc ccggcatacc
     4021 ccagggacac gatattggta gccaccgaga tgatcgagcg caacaatgat ttttcctcga
     4081 cgatccgcgc gtaatattcg atattggccg cggtgggcac cgagttagcc agggctgtca
     4141 gataggccac tcccccgatc ttgtccagca tctcgcggcg ttttagttcc tcagagacgg
     4201 tgatgatatc taccggttca cctttattgg ccagttgcgt gacgacctca aagatcaccc
     4261 ggtgcgcttc gcgatagaaa tcctcgggtc gtaatacctc ggaacccttg tagatagcct
     4321 ctttctcaag catcatcgag ccaagcgtgg aacgctcggc ttcaatattc tggggtggaa
     4381 cccggtctag cgtggcatcg ctcatgacat cctccgtgaa cttagataaa cttagataaa
     4441 aatgtatcgg ataatgtgct agaaggagaa aagggtaaaa tgaaagggta aagggtaaaa
     4501 agaagtgatt ttccactttt ccctttaccc tttaaaattg gaataggata gcccacgctt
     4561 tttgccccgc gccccccccg tgggggcgcg ctactcatgc ctcttgccca ttcctattgc
     4621 ctgttgccta ttgcctaatt cgcggttgct aaattctccg ccggtatgag cgcgtcggca
     4681 atgatctcct gaatcgtctc gaccggaatg atctggatat ccttaaggtc ggtgggaaca
     4741 tccttctcat tctctttggg aataaacacc ttcttcatcc cggcttgccg ggcgccgtaa
     4801 atcttctcaa agaccccgcc tactgccttg atcttcccct gaatcgaaac ttcccctgtg
     4861 accgcaatat tttgggggat tgcctgtttg gtcaggctac tgatgatcgc caaagtaatc
     4921 gcgaccccgg ccgacggtcc atcgatcttt ccgccgccca ctacattcac gtgaacgtcg
     4981 aaatcactca gatccatgcc ggccaccagc ctaaccacag acgcggcgtt aaaaaccgag
     5041 tctttcgtca tgctgcccgc ggtttcgttg aaacgaagcc ggcctttacc tttatcggaa
     5101 gcggcgaacg ccaccgcttc aatctccaaa accgaaccga caaacccgta aacccccagg
     5161 ccaaagatgc ggccgcactc ggtacgttcg gaaccccgga ccgtcagata aggagagagc
     5221 ctactgacct gaaatacctc cagcagatcc tcctgaccga tggtgatctc cccccgatcc
     5281 ggctgttgat ccttgttttt atataaagcc aagccatagg catccgccaa aataccggcc
     5341 gccttccggc cttcaatggt atattcggcg atcaactccg gaacctccgg cgcgatccgg
     5401 acctccaacc gttcggcggc gttggtcact attatctgaa tatcctgttg atccaagggt
     5461 tcgaagaaaa cctccgcgca gcgggaacgc agcgccgggt tgatctccga tggatcccgt
     5521 gtggtggcgg cgattagaat aaaatcggcc ggtgcacctt cggagaatag tttcttaata
     5581 tatttcggaa catttggatc gttgggatcg taataagccg agtcaaaact gaccctttta
     5641 tcctcaagta cctttagaag tttgttcaaa agcatcggat ccatctcacc gatctcatcc
     5701 aggaacaata caccgccgtg agcctccgtc actaaaccca acttcggctc gggtacgcca
     5761 ccctcggcca gatcccgctt ggccccctga tagatggggt catgcaccga gccgagcaag
     5821 ggattggtca cttcccgagg atcccaacgc agggtggtcc cgtctacttc gacaaatgga
     5881 gcgatctcct taaacggtgt gaagggaatc cgtttcgctt cttccagcac caaccgggcc
     5941 acagtagttt tcccgacgcc tggtgggccg taaagaatca catgctgcgg aaagggcgaa
     6001 gcgatcttgg acaacagcgc tttaaccgcc cgatcctgcc ctaccacctc attgagtttg
     6061 gttggtttca acgtctccag tgccgatttg gaaagattga tggaatcgag tttctccagt
     6121 tccgccaatt tcttaagggt atgaggatta tcgggaccag cggattcctg aaggatctga
     6181 atcttgatct ccttcaaata ctcctcatga cgctgttgca tgcgatcagc gatcttcttc
     6241 tcgatcttct cctccaccgt gcggcgggca attagatcgg cgatctcctc ctcaatctcg
     6301 tttagcactt gaggccgatc cttccggtgc ggcagggtat cgatggtcgg atcttcaaaa
     6361 acgatcttct gaagagccaa aacccgttct tctagaactt cggaacgcat cagtttcaag
     6421 gcctccaact taccggcctt tagaaccagt ttatccgagc catataaatt ggagagtaac
     6481 gcaaaaatcg cagccacctg gcgaaccaac tgttcgtcat cgagtagccg ctcgtgtaaa
     6541 ggaaccggcg gtttgacgcc gggagcaacc cttttgatta tgcctttcat cagtaactcc
     6601 ttttatagat ttaacccgaa tccggctgat atcaaaacat cgcctcaagg taacctgccg
     6661 cgatccggac gatttcacgc atcgatacat ggcgcgtaaa tgtgaattca ttaaatgcgc
     6721 gatgccttgt gggggtggtg taaaagtgca cgcaccccgg ttcaataaac tattttagta
     6781 aaaacaggtt aaaaaaggaa aaataatcca ataactacca ttcatgacaa atgattagac
     6841 ggaatggccg cccaagcata actattcggc gcttacctgt acgtgaatgg tcgcggtaac
     6901 ccctttatat agatggatct tgatgggatg atcacccacg gccttgatgg cttcgtcaag
     6961 ttcgagcttt ctcttgtcca gttcaaaatg gatcatcttc tggatctgat ccgcaatgtc
     7021 tttcgcggta acagaaccga aaagccgtcc gccctctccg gccttgacct tgaaatgcag
     7081 ggctaagcca ttgagtttaa ccgccatttc cttagcctcc gcctcgtcca gcaattcccg
     7141 gcggatcgca ctttgttttt ggtcttccaa caacttcata ttggcgggag tagcctccac
     7201 tgccaaacct ttggggagca agaagttttg ggcataacca tccgccactt gcttaacatc
     7261 acccttgcgg cccaaggctt ttacatcctg ttttaaaata agcttcattc ccaacacccg
     7321 cctacattcg atttggttcc ttcagatttc gttcccgaag gtaattctga aggttggtca
     7381 catcgccgta ccactcctca atctcgtgat cctgtagttg aggagcttgc aattgctgct
     7441 caatccgggc atccagtttc gcgaagggaa tacccagccg tttacccaaa aaataacaac
     7501 ttacgatcaa ccaggccaag gagtcctgga tcgcttcctc actgttttta accattgcct
     7561 tgaagagcga gcccaaaccg gccacgatct ccgcctttaa ccattcgata atccttacgt
     7621 tctttacgat attaaagtca gggttgttag acgacatgag aagttcctcc ttcaacccat
     7681 catattctct atcaccgttt tttttcctgc gtttgcaatt aggtgttccg cctgtttttc
     7741 cccacttaaa ccggttattc cggcctgttg agattcttgt cgggagctgt ctcaaaaaac
     7801 tcttccggca cagcctacgc cttctctcca actaacgcat tggatggcga aaggtttccg
     7861 ccacctaaaa aaagaggcct gaggcctctt tattcactgg taaatggtaa taacgcgatt
     7921 tggcgcgccc gcttgattgc cgacgtcaac tgacgttgat gaatggcgca attgccggag
     7981 atccgtcgcg gcagaatctt gcctctttca gtgatgaact tacgtaattt tgctgtttct
     8041 ttataatcga tatgttccac tttgtctaca caaaaggaac acaccttctt cttcggccgg
     8101 cgccctctgc cgggttcccg gtcgccgcgt tcccgttcgt ttctaggcat ttaaatcacc
     8161 tccacataaa ataacacttg actcggtaat cgttattttg taaaaaccgt tacacgcgca
     8221 tgcgcgtgac gagtcggttt tacgaaaaat caaaatggta gatcatccag gttaatgccg
     8281 cccagatctt cagcaggatc cgaacccggg tccccgccgg cattcgtcct gccgcctgac
     8341 gaaccggtcc ccggagtaaa tccgcttccg gttgatggcg caccggcatc atcacggccc
     8401 ttgtccaaaa attgaacaaa actcgcaatt acctcggcag cctttcgttt ctgaccgtcc
     8461 tgggtttcat aatttcgaat ctgcaaacgg ccctcaactc cgaccaaacg gccttttttt
     8521 aaataattgg cacaaagttc ggcctgtttt tgccaggcga cgatgcggat aaaatcggct
     8581 tcccgttcgc cttgttggtt aggtgtagga cgatctaccg ccagatcaaa gttggcgacc
     8641 gccactccgt tcggtgtgta acgtagctcc ggatctctgg tcaatctacc gatcaggaca
     8701 atatgattca tgaacgatca ccttcattcc tgcgtttaca acgtgcttgc gcattcttgc
     8761 cgccaaatc
//
LOCUS       DASVMB010000392         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_639584,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000392 DASVMB010000000
VERSION     DASVMB010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639584"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2617
                     /locus_tag="VF531_11090"
     CDS             <1..2617
                     /locus_tag="VF531_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX7714553.1"
                     /translation="NGNSQKRITMEVETGDAGDRYWKAGIGPFDFGDHVEYRFIVKTD
                     DTQVTSKAENFTVRAWQPVGDITNVRINSDRVTLDFNSLPGTAKRPCFEMKKHNDSTL
                     KWSFYVTDVSPVGDPVVAEDVNIELDNKILKIGTRDLSINVSDKFMQGPPAQILQSYN
                     RHGGGFIEFLGDGSDQIYKVRFKFFMDGAERFFGMGERYSHIEYRGHNVDTYVYNQYR
                     DQRLRTYIPVPLAISSKGYGIYTDTPMYSVFRFGTRLSDLFEMEINIHKEKQATAGYI
                     FTGNPKEIVQSFVKLTGKPQLPPKWSFGPWISSNNWDSQAETMKQVELTNKYKIPATV
                     LVLEQWSDEATFYIFNDAQYRVKDGNDYLRYEDFTYPDWGRWPDPRKMVEDLHQNGLK
                     VLLWQVPVEKYMDGIAHGQRDEDERAMLAGGYHVQYRDGKPYRIPDYEWFKRSLVLDF
                     SNPAAREWWFNKRLYLLKDIGVDGFKTDGGECIYGEDVLFFDGRTGDEMRNQYANDYI
                     GAYHRFAAEHVADGGVTFSRAGFTGAQQYPLHWAGDERSTYEAFRASVRAGLNCGMSG
                     IPFWGWDLGGFHGAIPSAELYIRAAQMAAFCPIMQYHAETKGEFNQDRTPWNIAERTG
                     RPLVIEIFKKYADLRMNLLPYIYQQAIQSAETGIPMMRPMVMEYPDDPSCMEVSQQYM
                     FGDSLLVAPVTEEDSSGKDVYLPDGDWINFFSGEIVTGNRLLRVKAALENIPVYLREN
                     SVIPLNLSDDFQLCSHVGNRVDRYENLCFMVFLTASANYDFRDDLGNKLTIGLAKSAH
                     EIRIDLKSNYSGPILLIFANISKIAAVEIINNPGSIDNNPAGYTPDRCTIRDNALNIK
                     VGHKNEGKASMIARI"
     gene            2824..>3098
                     /locus_tag="VF531_11095"
     CDS             2824..>3098
                     /locus_tag="VF531_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7714554.1"
                     /translation="MKVIALNGSPRKNWNTATLLRRALEGAISQGAETELIHLYDLNY
                     KGCISCLACKTRNGPSYGKCAVNDELAPVLRKVAAADAFILGSPIYVG"
BASE COUNT          873 a          634 c          792 g          799 t
ORIGIN      
        1 aaacgggaat tcacagaaaa gaataacgat ggaagttgaa accggcgatg cgggagacag
       61 gtactggaag gccggtatcg gaccgttcga ttttggcgat catgtcgaat acaggtttat
      121 cgtaaaaacc gatgacactc aggtaacttc caaagcagaa aattttaccg tcagagcgtg
      181 gcaacccgta ggggatatta cgaatgttcg tataaacagt gatcgagtca cattggattt
      241 taattcttta cccgggactg caaagcgacc ttgtttcgaa atgaaaaagc ataatgattc
      301 cacgctaaag tggtcatttt atgtaacgga tgtttccccg gttggtgacc cagttgttgc
      361 agaagatgtc aatatcgaac tggataataa aatcctgaaa atcgggaccc gtgatttatc
      421 gataaatgtt tcggataaat ttatgcaggg gccgcctgcc caaatacttc aatcttataa
      481 ccgtcatggt ggaggattta tcgaattttt aggagatggc agcgatcaaa tctataaagt
      541 tcggtttaag tttttcatgg acggcgctga aaggttcttt ggtatgggag aaagatattc
      601 tcatatcgaa taccggggac ataatgttga tacctacgtc tataaccaat accgggacca
      661 aagactcaga acctatatcc cggtacccct ggctatcagc tcaaaggggt acggaatcta
      721 tacggatacc ccaatgtatt ctgtttttag atttggcaca aggctctcgg atttgtttga
      781 aatggaaatc aatattcata aggaaaaaca ggctaccgcc ggatacatat ttaccggaaa
      841 tcccaaagag atcgttcaaa gtttcgtaaa attaaccgga aaaccgcaac tccctccgaa
      901 gtggtcgttt ggcccgtgga tttccagtaa caactgggat agtcaggcgg agacgatgaa
      961 acaggttgag ttgaccaata agtataaaat ccctgccacg gtcctggtgc tggagcaatg
     1021 gagcgacgaa gccacatttt atatttttaa cgatgctcaa taccgtgtga aagacggcaa
     1081 cgactatctg cgctatgaag attttactta tccggattgg ggaagatggc cggatccgag
     1141 aaaaatggtt gaagatctgc atcaaaacgg tttaaaggta ttgctatggc aagtcccggt
     1201 ggaaaagtat atggatggaa tcgctcatgg ccaaagagat gaagacgaaa gggcaatgct
     1261 cgccggcggc tatcatgtgc aataccggga tggcaaacca tatcgaattc ccgactatga
     1321 atggtttaaa agaagtctgg tgcttgattt ttcaaatcct gcggccagag aatggtggtt
     1381 taataaaaga ctgtatctgt tgaaggatat cggtgttgat gggtttaaga cggatggcgg
     1441 ggaatgtatc tacggggagg acgtgctgtt ttttgacggc agaaccgggg atgagatgag
     1501 gaatcagtat gccaatgatt atatcggcgc ttatcaccga tttgcggcgg agcatgtggc
     1561 tgacggtgga gtgacattta gcagggccgg ttttaccggg gctcaacagt atccgctcca
     1621 ctgggcggga gatgaacgat ccacctatga agcattcagg gcgtcggtga gagccggttt
     1681 gaattgcggc atgtccggga tccctttttg gggttgggat ttggggggat ttcacggggc
     1741 cataccttca gccgagctgt atatacgtgc cgcccaaatg gcggctttct gcccgatcat
     1801 gcagtatcat gccgagacca aaggcgagtt taatcaggac cggacaccgt ggaacattgc
     1861 ggaaagaacc ggaagacctt tggtaatcga aatatttaaa aaatacgccg acttgcggat
     1921 gaatctgttg ccttacattt atcagcaagc cattcaaagc gcagagacgg gtatccccat
     1981 gatgaggccg atggttatgg aatatccgga tgaccccagc tgtatggaag tatctcagca
     2041 gtatatgttc ggtgacagct tgctggttgc cccggttact gaagaagata gctccggaaa
     2101 ggatgtatat cttccggatg gggattggat caatttcttt agcggggaga tcgtcacggg
     2161 gaacaggctg ttaagagtga aagcggcact ggagaacatc cctgtttacc tcagagaaaa
     2221 cagtgtaata cctttgaatc tctctgatga ttttcaactg tgcagtcatg tcggaaacag
     2281 ggtagaccga tatgagaacc tttgtttcat ggtttttctg acagcttccg ccaactatga
     2341 ttttcgggat gatcttggaa ataaattgac gattggcctg gccaaaagcg ctcatgaaat
     2401 tcgtattgat ttaaaatcga attattcggg acccattctc cttatttttg cgaatatttc
     2461 caaaatcgcc gctgtagaaa taataaataa tcctggatcg atagacaata accccgcggg
     2521 gtacacaccg gaccgttgta ccattcgtga taacgcattg aacatcaagg tcggccataa
     2581 aaacgaagga aaagcatcga tgattgcaag gatttgacgc atccaacaat tataatcccg
     2641 ttcctccggt gcttactttt tggtaagtat atatctgaaa agtgcgtact tattttcttg
     2701 ataattaagc gatatacttt tcacaagtaa gcaatcagtc cgttgcgtac gggaggaatc
     2761 ggatttttca cctaatccgc cccggatcgt tgagttctga tacacggtga ggaggtgtca
     2821 ttcatgaagg taatcgcctt gaacggcagc ccgagaaaga attggaatac cgccaccctg
     2881 cttaggaggg ctcttgaagg agccatttct cagggagccg aaaccgaact gatccatctg
     2941 tatgatctga attacaaagg ctgcatcagt tgtctcgcct gcaagaccag gaatggtccc
     3001 agttacggca aatgcgctgt aaacgatgaa ctggcacccg ttctccggaa agtcgcagcg
     3061 gccgacgcat ttatccttgg ttcccccatt tatgtcgg
//
LOCUS       DASVMB010000393         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_344325,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000393 DASVMB010000000
VERSION     DASVMB010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344325"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VF531_11100"
     CDS             complement(<1..1153)
                     /locus_tag="VF531_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7714555.1"
                     /translation="MNEPSLKLLIVDDEAEIREGISSAVPWSENGIEPAGLASSGREA
                     LQFIHDLNPDMMLLDIRMPVMDGLEVLKQLSGMAHPPKVVILSGYDEFEYCQQALRFG
                     VSDYLLKPCLPKEILNVMTRVRNDILNERNHNIQWENLKEQFQESKDLLREKLLIYLI
                     QHDHIDMETALIRWQLYQMELVPGNLGVALVRIDNLFNLQHSSGQELELIKFAVRNLI
                     EEVFKKTVPTKQVICDYNDDLLILWNLDTIPLDSAEQILEQIRGKVESSLAFTVTIGL
                     GYPALDLAGLYSAFNSSFLAVEYSFWIGPNRIIRPEQIKAGESEETLLSLQDENEVIQ
                     SLRMNDIERLQPALDQFFDGLTHEPKLSREQFQKKVTALICSIYHVCLER"
     gene            complement(1146..2975)
                     /locus_tag="VF531_11105"
     CDS             complement(1146..2975)
                     /locus_tag="VF531_11105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7714556.1"
                     /translation="MRQLSPVRFFKEHAPALPLKRKISLFVALLLFLAIFAVGVLSYS
                     IAVDQVVEKVTESQLGLVRQVANNLDQLLNDAEDLSSLIVIDPGVQQNLQKQYLGPKP
                     LYYPDSFSYLDKIMAVKSYISMITIYGYNGLNYSVGTDVTGSSVVSFREFQKNPLFSR
                     AVALNGAIGIEYFNNIPQVTYDNRISRIVLYRMIRDINDYHNLGVLLVRLNENKLRAM
                     YQMSIPAGGSISIVDQRGTIISSSAPRPIVTKAFKLVPLNRLNPDGATAESVQINGKK
                     MLLTLGTSQLSGWKIVALTPAAILTEKISSIALVILMVSIVCYILMLVFSGYITSIIT
                     NPLQQLLGSIKKVQQGDFTQQVSFTGRDEIGELGRGYNAMLVHIRDLIERVYKLQIHE
                     REAELNALQAQINPHFLYNTLDTIFWKAEKNQVPEISEMVYALSKIFRLSLNRGNDLT
                     TVAQEQELIQYYLALQKIRFKEKLRYEINLEPDLLNLTIPKLILQPFVENAIIHGIEE
                     LENGGMILISGRLQDRMMIFTIRDNGVGMSEEKIARILWRGEIDSVNPAPVSGGYAIR
                     NVLERLELYYSSQYHLEFQSNPGAGTTVTIQIPTQMKQVSVNE"
     gene            3200..4486
                     /locus_tag="VF531_11110"
     CDS             3200..4486
                     /locus_tag="VF531_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714557.1"
                     /translation="MKRFWTLLLVVAVLCTSLAVNVFSAPAKITLRLGDNIPDRNHGW
                     GAIIEKINAEFKKAHPNVELVTESLQDQPYQQKIKIYAASNQLPDIMKYWSFSSMMPP
                     LIDNGYVMALNAKDWKKYNFLPGALEANTYNGKLYGLPVTADIWLMYYNKALFEKYNV
                     KVPTTLDELIAVSKVFRDNGVIPAITDGKDAWPLSLNICAILARQTGNYNTLAEQAFG
                     RKIKATSPAFLNAAKTYKELIDAKVFNDDLLTTDYGASRNLFGQEKAAMFFMGTWEMG
                     MSTDSSFSEHFRNNLAVANFPTVKGGKSDPSDTFCWFGGNYIINAKTKYKSLALDYLK
                     LYFSMYPALTWKDKATLPAQKVQTSSEDNNVAKAIVALANGAKSTYGPGLLDNSTPEY
                     KELDQKYIGELTAGIKTPEQWAQAIDEAMANAAKKK"
     gene            4628..5512
                     /locus_tag="VF531_11115"
     CDS             4628..5512
                     /locus_tag="VF531_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714558.1"
                     /translation="MNKVFSNKLAIFLMVLPGLAVLTFAMIAPIFLSAYYGMTDWSGL
                     GAPQFIGLSNFKEILLHDHTFYISLLNALYLTIVTVFLQNPFAILVCVAIISARKGGH
                     FFRTVYFIPAIISIVVTTKLWVFIFNPTYGMLNKVLGALGLNFLKLSWLSDLHTSIWA
                     VIFIVIWQGFGWAVLFYYAGLIGIPSELKEAARIDGASGLKLYTSIILPLMMPVIKSI
                     LVIDLISCFKQMETVFLSTGGGPGDVTQFLANYLYQKAFNYSQYGYGNALSVLFVIIC
                     IVGTVVLNKLTQKDVGEF"
     gene            5531..>6025
                     /locus_tag="VF531_11120"
     CDS             5531..>6025
                     /locus_tag="VF531_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714559.1"
                     /translation="METSLNSSGIGQVDRSAAPHRIKRILLYAFLIFIAAGQLFPLIW
                     LADYSLVKSSELFGSDFLKWPEPFQWINYQRAWVDGKILTYFINSAIVVGAAVILSVL
                     LSYMLSYACTRMDWKLSPVVYGFVMLGMMIPIHATLLPNFILFNKTGITDTYWALIIP
                     YVAFT"
BASE COUNT         1489 a         1482 c         1476 g         1578 t
ORIGIN      
        1 cccgttccag acagacatga tagattgagc agatgagtgc ggtcactttt ttctgaaact
       61 gctctctgga aagtttcggc tcgtgcgtca acccgtcaaa gaattggtcc agggcaggtt
      121 ggagccgctc gatatcgttc atgcggaggc tttgaataac ctcattttca tcttgaagcg
      181 ataataaagt ttcttcagat tcgccggcct tgatttgttc cggccggata atacgattcg
      241 ggccgatcca aaaactgtac tcaaccgcta aaaaggatga attaaaagcg gaatacagtc
      301 cggccaggtc aagggcggga taacccaatc cgatggtcac tgtaaatgcc agactgctct
      361 cgaccttgcc gcggatttgt tccaaaattt gttccgcgct atcgagaggt atcgtatcaa
      421 gattccagag gatcaaaaga tcatcgttat aatcacagat aacctgcttc gtagggacag
      481 tttttttgaa aacctcctca atcaggttgc ggacagcgaa tttaatcagt tccaactcct
      541 gaccggagga atgctgtaaa ttgaaaaggt tgtcgatcct caccagggcc actccaaggt
      601 tcccgggtac caactccatc tgatagagtt gccagcgaat taaggctgtt tccatgtcga
      661 tgtgatcgtg ttggattaag tagattagta atttttcccg cagcagatct ttactctcct
      721 ggaattgctc tttaagattt tcccattgta tattgtgatt tctctcgttt aaaatatcgt
      781 tcctgaccct cgtcatcacg ttcaggattt cctttggtag acagggtttg agcagataat
      841 cggagacacc gaagcgcaag gcttgttgac agtattcaaa ttcgtcatag ccgctcagga
      901 tcacgacctt gggagggtgc gccatcccgg ataattgctt taagacctcc aaaccatcca
      961 tgaccggcat ccggatatcc agcagcatca tatcggggtt taggtcatgg atgaattgca
     1021 aggcttccct gccgctactg gcgagtccgg ccggttcgat gccgttttcc gaccagggaa
     1081 cggcggagct gattccctcg cgaatctcgg cttcatcatc gacgataagt aatttcagag
     1141 agggttcatt cattgacgct cacctgtttc atctgagttg gaatttgaat ggtgacggtg
     1201 gttcccgctc ccggattgct ctgaaactcc aagtgatatt gagaactata ataaagctcc
     1261 agccgttcca ggacattccg aatggcataa ccgcccgata ccggagccgg gttgaccgag
     1321 tcgatctcgc cgcgccataa aatccgggcg atcttctctt cgctcatacc cactccgtta
     1381 tcgcgaatcg tgaagatcat cattcggtcc tgtaaccggc cgctgatcaa gatcattcct
     1441 ccgttttcca actcctcaat cccgtgaatg atcgcgtttt caacgaaagg ttgcaggatt
     1501 agcttgggga tggtcaaatt caataaatcc ggttcaaggt tgatctcgta gcggagcttc
     1561 tctttaaatc ggattttttg cagtgccaaa taatactgga tcaactcctg ttcctgggcg
     1621 acggtggtca gatcgttgcc ccggttcaaa ctcagacgga agatcttgga gagggcgtac
     1681 accatctcgc taatctccgg cacctggttc ttctccgctt tccaaaagat tgtatccagg
     1741 gtgttataga gaaagtgggg gtttatctgc gcttgtaagg cgttgagctc agcttcacgt
     1801 tcgtgaatct gtagcttgta tacccgctca atcagatccc ggatatgaac cagcattgca
     1861 ttgtatcccc ggcccaactc gccgatctcg tcccggccgg taaaactaac ctgctgtgta
     1921 aagtcgcctt gttgaacttt tttaatcgaa cccagtagtt gttgaagcgg attggtgatg
     1981 atactggtaa tatatccgga aaagaccagc atcaggatgt aacaaacaat actgaccatg
     2041 aggatgacca atgcgatgga gctgatcttt tctgtcagga ttgccgccgg ggtcagggcc
     2101 acaatcttcc agccgctcaa ttgggacgtg ccgagggtca aaagcatctt tttgccgttg
     2161 atttggacgc tttccgccgt agctccgtcc ggatttaatc ggtttagggg gacgagctta
     2221 aaagccttgg tgacgatggg ccggggagcg gaactggaga taatggttcc gcgctgatcg
     2281 acgatggaaa tactcccccc tgccgggatg ctcatctgat acatggctcg cagcttattc
     2341 tcgttcaagc ggaccaagag gactccgaga ttatggtaat cattgatatc cctgatcata
     2401 cggtacagga caatccggga gatgcggtta tcgtaggtca cctgggggat attgttgaag
     2461 tactcgattc cgattgcgcc gttcaaagcg accgccctgg aaaacagggg gtttttttga
     2521 aactcccgga atgagaccac gctgctgccg gtgacatccg taccgacgct ataattcaat
     2581 ccgttatatc cgtagatggt gatcatggag atgtaactct tgaccgccat aattttatcg
     2641 aggtagctga aggaatcggg atagtataag ggtttgggcc ccaggtattg tttttgaaga
     2701 ttctgctgca ctcccgggtc gatgacgatt agtgaagaga gatcttcggc atcgttcagg
     2761 agttggtcca gattgttggc cacctggcgg accaatccca gctgggattc ggttactttc
     2821 tcaacaacct ggtcgacggc gatggagtag gataaaactc ccactgcaaa gatggccaaa
     2881 aagagcagca aggccacaaa caggctgatt ttccgcttaa gcggcaatgc cggggcgtgt
     2941 tccttgaaaa atcggaccgg ggaaagctgg cgcaatttca ttcctccctg gatatcaatt
     3001 tgggcaaaat gtcgtgatta cctccaggga aatgataacg ttcgaaaaaa ttaaacctta
     3061 tttgaagatt aacccctttt cacgctccgg gaattcgagg ataatgataa caaacggtta
     3121 cgatagaatg aatgaaagat gaaaatcctg ttacattctt cttcgtaatt gctaaaaatt
     3181 tgaaggaggg gtaagttaca tgaaacgttt ttggacctta ctactggtgg tcgcggttct
     3241 ctgcacttca ctggctgtca acgtcttcag tgctccggca aagatcaccc tgcggctggg
     3301 ggataatatt ccggaccgga atcacggctg gggtgcgatc attgagaaga ttaacgccga
     3361 gttcaaaaag gcacatccca atgtggaact ggtgactgaa agcctccagg atcaacccta
     3421 tcaacagaag atcaagatct acgcagcctc caatcaatta ccggatatca tgaagtactg
     3481 gtctttctcc agcatgatgc cgccgctaat cgacaacggt tatgtaatgg cgctcaatgc
     3541 aaaagactgg aagaaatata atttcctgcc cggcgcttta gaggccaata cgtacaatgg
     3601 caagctctat gggctgccgg taaccgccga tatttggctg atgtactaca acaaagcgtt
     3661 gtttgagaag tacaatgtca aagtccccac cactttggat gagttgatcg ccgtatccaa
     3721 ggttttccgg gataacggtg tcatcccggc gatcaccgac ggtaaggatg cctggccgct
     3781 ctcgctgaat atctgcgcca tcctggcccg acagaccggt aattacaata ctttagccga
     3841 acaagccttt ggccggaaga tcaaggctac cagcccggct ttcttgaatg cagccaagac
     3901 ctataaagag ttgatcgatg ccaaagtctt caatgatgat ctgctgacca ccgattacgg
     3961 cgcgtcccgg aaccttttcg gccaggagaa ggcggccatg ttcttcatgg gcacctggga
     4021 gatggggatg tccaccgatt cctccttctc ggagcatttc cggaataacc tggctgtagc
     4081 caacttccct accgttaaag gcggcaagag cgaccccagc gacaccttct gctggtttgg
     4141 cgggaactac atcatcaatg ccaagaccaa atataaatcg cttgccttgg actatctgaa
     4201 actgtacttc agcatgtatc cggctctgac atggaaagat aaggccacgc tgccggcaca
     4261 aaaggttcag accagttccg aggataacaa cgtcgccaag gccatcgttg cgctcgctaa
     4321 tggcgccaag agtacttacg gccctggttt gctcgacaac tccactccgg agtataaaga
     4381 gttggatcaa aagtatatcg gtgagttgac cgccggtatc aagacccctg aacaatgggc
     4441 gcaagcgatt gacgaagcca tggcgaacgc agccaagaag aaataagaca ctttcccggt
     4501 ttggaagatg ggcagaagct gggcttctgc ccatcttata ttcggagccg aactatttct
     4561 ccccctataa aagggcttga gatgaaacgc atcaagttgt agacagtaca aaaagagagt
     4621 gatcgaaatg aacaaggtat tctcgaacaa gctcgccata ttcctcatgg tccttccggg
     4681 gctggcggtt ctgactttcg ccatgatcgc gccgatcttc cttagcgcct attacggaat
     4741 gaccgattgg tccggcctgg gtgcacccca attcatcggt ttgagtaatt tcaaggaaat
     4801 actcctgcac gatcatactt tttatatctc attactgaat gcgttatact taaccatcgt
     4861 taccgtcttt ctgcaaaatc ccttcgcgat tctggtttgc gtagcgatta tctccgccag
     4921 aaagggcggg catttttttc ggacagtcta ctttattccg gcgattattt cgatcgtagt
     4981 caccacgaaa ctctgggtct tcatcttcaa tccaacctac ggtatgctca ataaagtgtt
     5041 gggcgccttg ggacttaact ttctcaagct gagctggctg agtgatctcc atacctccat
     5101 ctgggcggtg atcttcatcg tcatttggca aggttttggt tgggcggtcc tcttttacta
     5161 cgccggcctc atcggcatac cgagtgaact gaaggaagca gccaggattg acggggccag
     5221 cggtctcaaa ttatatacct ccattatcct tcctttgatg atgccggtga tcaaatcgat
     5281 cctggtgatt gacctgatct cctgcttcaa gcagatggag acggtgtttc tctcaaccgg
     5341 cggcggtccc ggtgatgtca cccagtttct ggcgaattat ttatatcaaa aagccttcaa
     5401 ttatagccaa tacggatatg gcaacgcgct ctccgtattg ttcgtgataa tctgcatcgt
     5461 cggtaccgtt gtactgaaca agcttaccca gaaggatgta ggggaattct aagcgagagg
     5521 aagcatggac atggaaactt cgctaaactc ctccggtatc ggtcaagtgg accgttccgc
     5581 cgcaccacac cggatcaaaa gaattttatt atatgcattt ttaatcttca tcgccgccgg
     5641 gcaactcttc ccccttatct ggctggccga ctactccctg gtgaaaagca gcgagctttt
     5701 cgggtcggac tttttgaaat ggccggagcc gtttcaatgg atcaactacc aacgcgcttg
     5761 ggtggacggg aagatactca cctattttat taacagtgcc attgtggtcg gtgccgcggt
     5821 aatcctgtcg gtactcctct cctatatgct ctcttatgcc tgcacccgga tggattggaa
     5881 actcagtccg gtggtgtatg gatttgtgat gttgggaatg atgattccga ttcatgccac
     5941 tttattgccg aacttcatct tattcaacaa aaccggtatc acggatacct attgggccct
     6001 gatcattcct tacgtggcat ttacg
//
LOCUS       DASVMB010000394         8729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_490153,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000394 DASVMB010000000
VERSION     DASVMB010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490153"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..1501
                     /locus_tag="VF531_11125"
     CDS             521..1501
                     /locus_tag="VF531_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714560.1"
                     /translation="MKKLLPISLTVMLILAVALSGCGGGKSSPANPESSNTTTIYAGI
                     FNTGTTASLLKSTDGGNNFAELTSFAAGSAGTIWQIFGSANSIYVATSKGLAISTDNG
                     ATFNWYLSGDSVRSVYVSGTTIYAGVIAVSSATNRLAISNDGGKSWTVSLDSYQINTV
                     HGSGSTICVATITGLAVSTDSGANFKLYDLAGDTVNSVYVSGGVIYATTNSGLSISSD
                     NGSTFNTKLSGTTLASIAVSGSNVYTTSYNTATSSLELLVSHDSGDSFASKTTSDVSI
                     SGYPYLIWLSDSTVYVSGSTSGVSVSYDNGGSFTSVSGLSSLKVTSLFVK"
     gene            1613..2410
                     /locus_tag="VF531_11130"
                     /pseudo
     CDS             1613..2410
                     /locus_tag="VF531_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(2430..2612)
                     /locus_tag="VF531_11135"
     CDS             complement(2430..2612)
                     /locus_tag="VF531_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7714561.1"
                     /translation="MALLSISSFWKIWTHETLYETRVVDSHIRRKLKETGVSPELIET
                     VRYIGYRFTDVEDAPV"
     gene            2954..3088
                     /locus_tag="VF531_11140"
     CDS             2954..3088
                     /locus_tag="VF531_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714562.1"
                     /translation="MSYIKAGVPMKDYQLFMEIESGSSVTVDFSVKLHTIIFLSGIFN
                     "
     gene            3117..3278
                     /locus_tag="VF531_11145"
     CDS             3117..3278
                     /locus_tag="VF531_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714563.1"
                     /translation="MADETLFQNVKTDGDYDIPLREYLIEVPLQYVIWGGGRLRLTVN
                     ELMDVVLLG"
     gene            3439..4329
                     /locus_tag="VF531_11150"
     CDS             3439..4329
                     /locus_tag="VF531_11150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7714564.1"
                     /translation="MKKLLPISLTVMMILAVALSGCGGGNNGDNPPTLQPVSITESLV
                     AGAAGLTVTYPYYMAQTEVTYQIWSTVKNWATANGYTFANPGSCGGYYNKSTGLDETY
                     TSDRDTDPVTEINWRDAIVWCNALTEYYNAKNGKSWSCAYTSGGAPIRDSQNNTGVID
                     NLTDDGHTAKGFRLPTDAEWYLAARYIDGSTWTPDNYASGATAGNANATKEVAWYYEN
                     SESSTHPVGLKNANALGIKDMSGNVIEWCFDISGSGRLERGGCWFNDADMVTVGGSLF
                     INCLAGDISNAVGFRPVRTQ"
     gene            4827..4970
                     /locus_tag="VF531_11155"
     CDS             4827..4970
                     /locus_tag="VF531_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7714565.1"
                     /translation="MDPKIKNVYLKHVQSIGAAIQNILLAAHELGLATCWIGEIRTTG
                     GFK"
     gene            5292..6020
                     /locus_tag="VF531_11160"
     CDS             5292..6020
                     /locus_tag="VF531_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7714566.1"
                     /translation="MEFYTIKVDIRENKIGIITLSRPEKRNAISIQMRKEISYCLREW
                     REASTIDALIFIGNGSSFSGGYDLDEFGNPDCFPELLKSSTEYCLDIWTFPKPTIVAV
                     NGSAMGGGFDLLTLCDIRLCSESAIFGHPEIKFGGLPFFTPLRWIIGEGRARELCLTG
                     RKIDAQEAYQIGLVSRVLKDNDLLEHAIQLGKTILEAPTDALRFAKESFISNSGRGFE
                     ESFIIEHDEAFRKVLLPKAEKGFK"
     gene            complement(6277..6903)
                     /locus_tag="VF531_11165"
     CDS             complement(6277..6903)
                     /locus_tag="VF531_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX7714567.1"
                     /translation="MQVNYILDLFGTFAFALSGALMAIKKNMDLFGMIVLALVTAIGG
                     GTVRDMMLGLTPVFILTQPVYIYVSIIATISVFLFYKLFFRINSVILIADAIGLGTFV
                     CIGISKAIDTHVSLTGAVLLGLITAVMGGIIRDLLANEIPTVLIRDFYATACILGGVV
                     YVLLFKMGVPHNIIILVTAGFIIGLRMLAIKFNWNFIKVPYRFGVKGI"
     gene            complement(6954..8105)
                     /locus_tag="VF531_11170"
     CDS             complement(6954..8105)
                     /locus_tag="VF531_11170"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEX7714568.1"
                     /translation="MNRMEQDLQGWIPRLVAEWRRMAGIRGAGLPTARLTRNETDQVG
                     TALLRLQRGLTGSRTLIGGGYMNDPGSLGAYLLYYWPVSYLQTRYALVESGMHPRKLL
                     DLGSGPGAATLACIDAGAVTALAADRSRDALGVAGRLARAGGVKIETTSWNGESGAPL
                     PPGSYDCIILGHVLNELWVERSDRIERRLALLERISGHLGPAGRILILEPALLATSRE
                     LLHLRDRLMERGFQILAPCLFPGPCPAFAAGSDSTCHSEWAWELPPLVRDLARAARLD
                     RHSCKTTYLVISRSTPSGAGLAGVFRVVSDPMLNQAGRTRYMICGLEGRFSLSAKPGE
                     AGSAERSFFSLKRGDLIRVAHTETRGSGRGVTPETVLEVLKPGSKGKVK"
     gene            complement(8341..>8729)
                     /locus_tag="VF531_11175"
     CDS             complement(8341..>8729)
                     /locus_tag="VF531_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714569.1"
                     /translation="VGPLLGTLYFLTLGLVIKDDLPPLTAFHQSLAWLHRKPLSIWLY
                     SLLVSFLAGLGAFACFIPVILTMPFGTAGMAVAYHELSRQIVDPNRLLERESFGRTMR
                     IVGIVVAILFIIGLFSWGWKWPHFPY"
BASE COUNT         2461 a         1943 c         2132 g         2193 t
ORIGIN      
        1 gggatttttt tgatcctccg ctaaaggagg caggcgcatg agctatatca agaccgtcgt
       61 ccccatgaag gattacaggc tgtttatgga gatggagagc ggcagcagcg tgattgttga
      121 ttttaagtga aactgcacac catgatattc ctttcagaaa tatttaattg aagcctgctt
      181 caatacgaaa tacgcggggc ttgcggacga aactctgttt caaaacatca aaaccgacgg
      241 cgattatgat attcccttgc gggaatattt gattgaagtc ccgcttcaat acgtcatctg
      301 gggcggtgga cgcctgcggc tgaccgtgaa cgagctgata gaggtggttt tactgggata
      361 gccggaataa aaattgattt actataaaag ggtaaaacga aaaataccta attcaccacg
      421 aagaacacga agcgcacgaa gaagaaaaag ctttatctgc cgaatctttg ctgcgaaagt
      481 taagatttag aagaaaaata aattaaagga gatttttatc atgaaaaagc ttttacctat
      541 cagtttgacg gttatgctga ttttagccgt cgccctctcc ggctgcggcg gggggaaaag
      601 ttcaccggct aatccggagt cgtctaatac gactacgatc tatgcgggaa tatttaatac
      661 aggtactacg gcatcattac tgaagtcaac cgacggcggc aataattttg ccgaattgac
      721 tagttttgca gctggttcag cgggcaccat ttggcagatt ttcggatccg caaactccat
      781 ttatgtggcg accagtaaag gattagccat atcaaccgat aacggcgcta cttttaattg
      841 gtatttaagc ggtgattcgg tcagatcagt ttatgtatcc ggtactacaa tttatgcagg
      901 agtaatagca gtcagcagcg ctactaacag gttagccata tccaatgacg gcggcaaaag
      961 ttggactgtt tcgctcgatt cttaccaaat taatactgtt cacggctcag gaagcaccat
     1021 ctgtgtagcg actattactg gtttagcggt atcgaccgat agcggcgcta attttaaact
     1081 atatgatttg gcgggtgata cggtcaacag cgtatatgta tccggcggcg taatttacgc
     1141 gacaaccaat agcggattat caatatccag cgataacgga agcaccttta acactaagtt
     1201 atccggaacc actctggcct caattgctgt atccggcagt aatgtttaca cgacatccta
     1261 caatactgcg acaagttccc ttgaattact ggtatcccac gatagcgggg atagttttgc
     1321 cagtaaaaca acaagtgatg tatcaatttc aggttatcca tacctcatct ggttatcgga
     1381 cagcactgtt tatgtaagcg gttccacaag cggagtatcg gtatcgtacg acaatggcgg
     1441 cagttttact tccgtgagtg gtttgagtag tctcaaagta accagccttt ttgttaaata
     1501 aaacaaactg attataaaga ttaatacctg aaatatcaaa ggaggtattc acaatgaaaa
     1561 aaatttgttt ttatagttca atggctgtgt ttgctccacg gtaacttatc cttactatat
     1621 ggcccaaacc gaggttacct atcaattatg gtccaccgtc aaaacttggg cgactaccaa
     1681 cggatatacc ttttccaatc agggcacttg cggtgcatat attgccggtt ctgattcaaa
     1741 cggttattct attatcgaac actatacatc cggccctgag actcaaccgg tcactaatat
     1801 ctcttggcgg gatgcggttg tctggtgcaa cgcgttgacc gaatattaca atgcgcaaaa
     1861 cgggacgagc ctggattgcg tctacacatc cggcggaact ccgatccggg attcaacgaa
     1921 taataccagt gttatcgaca acctgacgga tgacggtcat accgccacag gatttcgcct
     1981 gccgacatcc atggaatggg agttggcggc cagatacatc ggatctacca aaccaacagt
     2041 tgcgccgtta agtaacgaag ttatccagat taatttctat tggactcccg gcgattatgc
     2101 cagcggagca accgctaatt atcagaatac taatgcgaca tatgctgtaa gctggaactt
     2161 tgataacagc ggagagtcta ctaagccagt aggcaactta gcagccaacg tcctggaaat
     2221 tcacgatatg agtggaaatg tctgggggta ttgctttgat caagtatcta gtgaaaggaa
     2281 actgattagg ggcggaggcg ttttttcctc taatgaagcc ttggcaataa gtccaactgg
     2341 acaaggatat gatacgcttc ccagtgatat atccgattcg actggtctca gaccggtaag
     2401 aaccaagtaa aataatgatc ttttaaattt caaaccggag cgtcttccac atcggtaaaa
     2461 cgatacccga tgtagcggac agtctcgatc aattccggcg agaccccggt ttccttcagt
     2521 ttacggcgga tgtggctgtc gacaacgcgg gtttcatata aggtttcatg agtccagatc
     2581 ttccaaaaac tcgatatcga tagcagcgcc aagctggcag agaagctgaa atagcgttaa
     2641 tattaacaag gccatttatt accacgaaga gcacgaagga gaactaaaag gagcaggaag
     2701 attaagtttg atgtcttatc gaaccgaatt attggttatg tgattaaggt tcatctgatg
     2761 tggagcgttt gaaagatggc ctaagacggt ttgtttttaa attcttccta ctcttggtgt
     2821 ccttcgtggt agattaaaca gttccctttt tccaaaagat tgtccattcg gacgatgcgc
     2881 aatttctaat cccatcttac aatttttgta ggatgggatt ttttattgat cctcccccaa
     2941 aggaggcaag cgcttgagct atatcaaggc cggtgtcccc atgaaggatt accagctgtt
     3001 tatggagatt gagagcggca gcagcgtgac tgttgatttt tcagtgaaac tgcacaccat
     3061 aatattcctt tcaggaatat ttaattgaag cctgcttcaa tacgaaatac gcggagttgg
     3121 cggatgaaac gctgttccaa aacgtcaaaa ccgacggcga ttatgatatt cccttgcggg
     3181 aatatttgat tgaagtcccg cttcaatacg tcatctgggg cggtggacgc ctacggctga
     3241 ccgtgaacga gctgatggat gtggttttac tgggatagcc ggaataaaaa ttgattttac
     3301 tataaaaagg gtaaaacgaa aaatacctaa ttcaccacga agaacacgaa gcgcacgaag
     3361 aagagaaagc tttatctgac gaatctttgc tgcgaaagtt aagattagaa gtaaaaaaaa
     3421 ttaaaggaga tttttatcat gaaaaagctt ttacctatca gtttgacggt tatgatgatt
     3481 ttagccgtcg ccctctccgg ctgcggcggg gggaataatg gtgataatcc gccgacgctg
     3541 cagccggttt ccattaccga gagtctggtg gctggagcgg cgggccttac tgtaacctat
     3601 ccttattata tggcccagac tgaggttact tatcagatat ggtcgacagt taaaaattgg
     3661 gcaaccgcaa atggatatac cttcgccaat ccgggtagct gtggcggata ttataataaa
     3721 agtacgggtc tcgatgagac ctatacttcc gatcgcgaca ccgacccggt aactgaaatc
     3781 aactggcggg atgcaatcgt ctggtgtaac gcgctgacag agtactataa cgccaaaaac
     3841 ggcaagagtt ggagttgcgc ctatacatcc ggtggggccc cgatccggga ttcgcaaaac
     3901 aataccggtg ttatagacaa cttgacggac gacggccata cagccaaggg ttttcgtctg
     3961 ccgaccgacg cggaatggta tctggctgcg cggtatatcg atggttcaac ctggactccg
     4021 gataattacg ccagcggggc gacggcgggc aatgccaacg ccactaagga agttgcctgg
     4081 tattatgaga atagtgaatc ttccactcac ccggtaggcc taaaaaacgc caacgcattg
     4141 ggaattaaag atatgagcgg gaatgtcatt gagtggtgtt ttgacatcag cggcagcggc
     4201 cgactggaac gcggcgggtg ttggtttaat gacgcggata tggtgacggt aggcggcagc
     4261 ttgtttatca attgtctggc tggagatatc tccaacgctg tcgggttccg tcccgtgagg
     4321 acccagtagg aaaccaagaa atagataact agggctgaat tttgacagac gcaccgctgc
     4381 tagcaacgtt gaaaccatgg tagttcttaa tgtgtttaaa gggtaaaaaa atactaacga
     4441 aataaggagc cgccttcttt agcggctcct tatttcgcat gcccaattaa cctagccaac
     4501 gtttccatga ttgcctcctt tgaaggaaaa cctacgtctt gggatgcgat ggcctcatac
     4561 tttaataccg gtttgattgc tttctcaaac catacaagtt cgcgggaaaa ttcatttcaa
     4621 tgagtatccc gccttgtaag cccaagcaca tccaactttg aagttgcaag ataaatctta
     4681 aagaaatggc tgtaactttg tggaataggt tctcggtagt aatgaataat aaaaatctag
     4741 taaaagaatt atcatctctt actgtatttt gtaattgggt acgaaattcc tcttgtttaa
     4801 tctcagtatt ccttgatacc gaagctatgg atcccaaaat aaagaatgtt tatttaaaac
     4861 atgtgcaatc aattggggct gcaatacaga atatactgct tgctgctcat gaactgggtt
     4921 tagcaacctg ttggattgga gaaataagaa ctactggggg tttcaaatga tctgcaatta
     4981 atggctgtta tagctgttgg tttttcagat aaaataattc aaaatccaaa agaaggaata
     5041 tttcagaaaa tattgtaagt tggtcataaa agcttctgat cttggctttg ggggctcggt
     5101 ccatcgtatc agcaagggtt cccgcttcgg acgcggtgat tcgaagaagg gtattcaaag
     5161 aagaaagttg taccacgaag ttgattcaag gagcagattc acgaaaatcg cgccacatca
     5221 tcaaagctaa gataggagct atctaagctt tgatggcatc ggatgataaa agcaatggag
     5281 ggaaatactg aatggaattt tacaccataa aagttgatat tcgtgaaaat aaaattggaa
     5341 ttattacatt aagccgccct gaaaaacgaa acgccatcag tattcaaatg cgaaaagaaa
     5401 tttcatattg ccttcgtgaa tggagagaag catcaaccat tgacgcctta atatttatag
     5461 gaaatggttc ttcgttctcc gggggatatg atctggatga gtttggcaat ccagattgtt
     5521 ttccagagct tttaaaatca tcaacggagt attgtttaga tatatggacc tttcctaaac
     5581 ctacgattgt agctgtaaat ggttcagcga tgggtggagg gttcgatctt ttaactcttt
     5641 gtgatataag attgtgttcg gaatcagcta ttttcggaca tccggaaatt aaattcggag
     5701 gtcttccttt ctttacccct ctacggtgga taatcggaga gggacgtgcc cgtgagctat
     5761 gtcttactgg ccggaagatt gacgcacaag aagcgtacca aataggactg gttagtcggg
     5821 ttcttaagga taatgatttg cttgaacatg cgatccagtt aggaaaaaca atacttgagg
     5881 ctccgaccga tgcgctaaga tttgctaaag aatctttcat cagtaatagc ggcaggggat
     5941 ttgaagaatc atttatcatt gaacacgatg aagcttttcg gaaggttctt cttcccaagg
     6001 cggaaaaggg atttaagtga ttgccgactg gctaaccgtc ctggttcagc gagcgtgtaa
     6061 gctaaactgt atgtgaaatc cggtacgtcg gagaacggct ccgttcttgc ttcggggctc
     6121 ggatagtaaa agaaggttaa cgcgaagaag ttgtaaattc atgatgtatt tcaatgaagt
     6181 ttattcatga gttcagaaac agattgcggc agggagagag tcttaagaga gacttcgttg
     6241 ggccctcctc cttgcctccc cctcgaagtc agcccctcat atccccttga ctccaaaccg
     6301 atacggaacc ttgatgaagt tccagttgaa tttgatcgcc agcatccgca ggccgatgat
     6361 gaatccggca gtcaccaaaa taattatatt atgcggaact cccattttaa aaagaagcac
     6421 gtaaacaact cccccgagaa tacaggcagt tgcgtaaaaa tcacgtatta aaaccgtagg
     6481 tatttcgtta gccagtaaat cccggatgat cccgcccatt acggcggtga tcaaccccag
     6541 cagcaccgct ccggtgaggg agacatgggt atcaattgct ttggagattc caatgcaaac
     6601 aaaagtcccc aagccgatgg catcggcgat gagtattacg gaattgattc gaaaaaacag
     6661 tttataaaag agaaaaacgc taatcgtcgc gattattgaa acatagatat aaacgggttg
     6721 ggtaaggatg aatacggggg tcaaacccag catcatatcg cgtacggttc cgccgccgat
     6781 agccgtaacc aatgccaata caatcatacc gaataagtcc atgttttttt tgattgccat
     6841 cagcgccccg gagagtgcaa aagcgaaagt gccgaaaaga tcaaggatgt aattaacctg
     6901 cattttgttc acctttttgt tttgtggaat aaagaatttg gtgcaacgat atcttactta
     6961 acttttccct tgctccccgg tttcaacacc tccaaaactg tctcgggggt taccccccga
     7021 ccactgccgc gggtctcggt atgggcaacg cgaatcagat cgccgcgttt caagctaaaa
     7081 aaggaccgtt ccgccgaccc cgcctctccc ggcttggccg ataggctgaa gcggccttca
     7141 aggccacaga tcatataacg ggtccgcccc gcctggttga gcatgggatc ggataccacc
     7201 cggaacacac cggcaagccc cgccccggac ggggtcgatc tggatatgac caaataggtt
     7261 gttttgcagg aatgacgatc cagccgggcg gcgcgggcca agtcccgcac cagcggcggt
     7321 aattcccaag cccattcgct atggcaagtg gaatcggatc ctgcggcaaa tgccgggcag
     7381 ggacccggga acagacatgg cgccaggatt tgaaagccgc gttccatcaa gcggtctcgc
     7441 aaatgtagca actctcggga tgtggcaagt aatgccggtt cgaggattaa tatccgaccg
     7501 gccggtccaa ggtgtcctga tatccgttcc agcagggcga gacgacgttc aatcctgtcg
     7561 gaccgctcaa cccataattc attgagaaca tgtccgagga taatgcagtc gtaacttccg
     7621 ggaggcagag gcgcgccaga ctctccgttc caggaggtcg tctcaatttt gacgccgcca
     7681 gcgcgggcca ggcgccctgc aactccgagc gcatcccggc tgcgatccgc tgccagggcc
     7741 gttaccgcac ctgcatcaat acaggcgaga gtagcggcac cgggaccgga acccaaatct
     7801 aaaagtttcc ggggatgcat tccggactca accagggcgt accgggtctg gagatatgac
     7861 accggccagt aatataaaag ataggctccg agggagccgg gatcattcat atacccgccg
     7921 ccgatcaagg tccgcgatcc ggttaagccg cgttgcagcc ggagtaacgc cgtacctacc
     7981 tggtcagtct cattacgtgt cagtctagct gtgggcagtc cggcaccgcg aatcccggcc
     8041 atgcgccgcc attccgcgac cagtcgtgga atccagcctt gtaaatcttg ttccatacga
     8101 ttcatcggct tatcctcatc aaccccgtcc tgcggttata ctgggaaacc ggcgggaggt
     8161 cgagaaaaag tggtgtttaa aatgcaaatt tattataaca tggttagggc ttattcgata
     8221 ccccggcaaa agccgattta tgccatttca acaagatacc cggccgaagg ccgcaacagg
     8281 cggtctcaca gccgggtaag aggtccgggg cattaacttg ttacgaagac tttggtgact
     8341 tcaataggga aaatgcggcc atttccagcc ccaactgaat aagccgatga tgaacaagat
     8401 agccacaacg atccccacaa tacgcatcgt ccgtccgaat gattcccgct caaggagacg
     8461 attgggatct acaatttgtc gggaaagttc atgataggcg acggccatcc cggctgttcc
     8521 aaacggcatc gtcaggataa ccgggataaa gcaggcgaag gcgcccaacc ctgccagaaa
     8581 cgataccagc aggctatata gccagattga caacggtttt ctatgcaacc aagccagact
     8641 ttggtggaat gcagtcaggg gcgggagatc atctttgatg accaaaccaa gggtgagaaa
     8701 atacaaagtg ccgagcaggg ggcctacaa
//
LOCUS       DASVMB010000395         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_490356,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000395 DASVMB010000000
VERSION     DASVMB010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490356"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF531_11180"
     CDS             complement(<1..268)
                     /locus_tag="VF531_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX7714570.1"
                     /translation="MTKRQAIARARVITGIAIFALMSLALWVSGAEAAGVTTRVKDIA
                     RLQGVRANQLSGMGMVVGLNGTGDSSRANIQMVTGVLTKMGYNNS"
     gene            complement(392..991)
                     /locus_tag="VF531_11185"
     CDS             complement(392..991)
                     /locus_tag="VF531_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX7714571.1"
                     /translation="MKNTRLKNGLIFGLTLILINAVGIAVQADSLWTDQSSSLYSGKP
                     RNFEVGDLLTVAVVEQAKATQQAQSSNNETGEVGAGPGTGLLSQLMPAMGGSWESDYK
                     GTGQTTRGGSLTANITVQVKEVSPNGLLAIEGRQVIRVNKEEQVIIISGLVRADDVSN
                     QNIVLSTKVANAVIEFQGKGTVSETQEPGLLVKFFHWLF"
     gene            complement(988..1899)
                     /gene="flgA"
                     /locus_tag="VF531_11190"
     CDS             complement(988..1899)
                     /gene="flgA"
                     /locus_tag="VF531_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX7714572.1"
                     /translation="MVTTRASGGVGPVFVIPDEVTVSGPDIHLSDLGKLQNGTDGDRE
                     NLKMINLGAAPLPGQIRKLSRDYLQIILRQHQTTQNMILEMGDQVEIRVTAVCIKAAD
                     LQAAVEKLIPDKKSQIIKKWLELRNLPEAIWLSNKDWRIEATPVRGIPEFGAVLFKVT
                     LTNGNERRMLNLSGRIRATALVFKAQRDLPRFAELNPGDFEAIESELTNGAELLGTIP
                     SGMRTTKPFRRGEILLSDQIQKVPLVNKGHEVGVIVKSNRLEIRITGIARSDGWLGDW
                     IQLQNPSSRKFFRGRVIGKDLVEVVPK"
     gene            complement(1979..2770)
                     /gene="flgG"
                     /locus_tag="VF531_11195"
     CDS             complement(1979..2770)
                     /gene="flgG"
                     /locus_tag="VF531_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX7714573.1"
                     /translation="MMRSLWTAGTGMIAQQTNIDTISNNVANVNTIGFKKIRAEFEDL
                     LYQTLRAPGDSTQAGTGQLPAGLQVGLGARLAATNRLFTQGNVQQTGNPLDLMIEGDG
                     FFQVQLPDGRIAYTRSGNFKNDGEGRLLNNDGYYLQPDISIPDGATDLSVAPDGTVTV
                     MMPGETLPQEIGQIELVRFVNPAGLLPEGKNLFLQSSASGEPVNATPGTEGVGAIVQG
                     ALEMSNVQVVEEMVNMIVAQRAYDVNSKAVQASDEMLQVANGLKR"
     gene            complement(3117..>3575)
                     /locus_tag="VF531_11200"
     CDS             complement(3117..>3575)
                     /locus_tag="VF531_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX7714574.1"
                     /translation="DLAMRGDGMFAVQAQNGIRYMRAGNFMVDKDRFLVSQEGLKVLG
                     QNGPIQLQEGAFQVTESGEIIQNGQIVNRLQVYSTAGMEKEGEYLYTNESPVPATDFQ
                     VIQGAVEHSNVNTVYEMIQMITATRGYETNQKALTAHDDTLGKLVNELAR"
BASE COUNT          809 a         1031 c          777 g          958 t
ORIGIN      
        1 ctgaattatt gtaacccatc ttagtcaata cacccgtgac catttgaatg ttagccctgg
       61 aactgtcgcc ggtaccatta agtcctacca ccatacccat tccggatagc tggttggccc
      121 ggacaccttg caacctggcg atatctttca cccgggtagt aactcctgcc gcctcggcac
      181 cggataccca taatgccaga ctcatcaggg caaatattgc aataccagtg attactcgcg
      241 cacgcgcgat cgcttgtctt ttggtcattc catccaccgc cttatgaaag gacgattgat
      301 aactcctcaa tattttatcg gagaatttct caatatcctt gataagccct tggtcgcttt
      361 ttttaacgga tcgaacggtc ttcaatttta attagaacaa ccaatggaaa aatttcacca
      421 acaaccccgg ttcctgcgtt tcacttacgg tacctttacc ctggaactcg atcaccgcat
      481 tggccacttt ggtggagagc acgatatttt gattggagac atcatcggcc cgtaccagac
      541 cactgatgat gatgacctgc tcttccttgt taacccggat tacctgccgg ccttcgatgg
      601 ccagcagccc attggggctg acctctttta cctgaaccgt gatattcgct gtcaaactcc
      661 cgccacgcgt tgtttgaccg gttcccttat aatccgattc ccaactgcca cccatcgccg
      721 gcatcagctg agatagaagc ccagttccgg gaccggcgcc gacctctccg gtctcattat
      781 ttgaggattg tgcctgttgg gtggccttgg cctgttccac aacggcaacg gtgagtaagt
      841 cccccacctc gaaattacgt ggtttcccgc tatataatga actgctctga tcggtccata
      901 aagagtcggc ttgaaccgca atccccacag catttatcag tatgagggtc aatccaaaaa
      961 tcaatccgtt tttcaatcga gtgtttttca tttcggtacc acctccacta aatcttttcc
     1021 gatcaccctg ccccggaaaa acttacgact tgagggattc tgtagttgta tccagtcgcc
     1081 cagccaaccg tcactccttg caattccggt gatccggatc tccagacggt tgctcttcac
     1141 gataactcct acctcatgcc ccttattaac cagaggaacc ttctggatct gatcgcttag
     1201 cagtatttcg ccccgacgga acggtttcgt cgtccgcatg ccggagggga ttgtccccaa
     1261 caactcggcg ccattcgtta gctcactctc aatcgcctca aaatctcccg gattgagttc
     1321 cgcgaatcga ggcagatcac gttgggcttt aaaaaccaag gcggtggcgc ggatacggcc
     1381 gctgagattc agcatcctcc gctcattacc attggtcaat gtcaccttga acaataccgc
     1441 tccaaactca ggaatacccc tgaccggcgt ggcctcgatt cgccagtcct tattacttaa
     1501 ccagatcgct tccggcaggt tgcgaagttc gagccatttc ttgatgatct gggatttctt
     1561 gtcgggaatc aatttttcaa cggctgcttg tagatccgcg gccttaatac agactgccgt
     1621 cactcgtatc tcgacctgat cccccatctc caaaatcata ttttgggtag tttgatgttg
     1681 cctgagtata atttgcagat agtcccggga cagctttcga atctgacccg gtaacggtgc
     1741 agctcccagg ttgatcattt ttaaattttc ccgatctcca tccgtaccgt tctgcagttt
     1801 gcccaaatca ctaagatgga tatccggtcc gctaaccgtc acttcatccg gaatgacgaa
     1861 aaccggcccc accccgcccg aggcccgggt ggttaccatc ggcacgaatc ctatgcaaaa
     1921 tatgataaaa tacatcacga gcttcggaat caaacccggt cggatcattt ctttaccctt
     1981 accgtttcag cccattggca acctgcagca tctcatcgga ggcctgaaca gccttggaat
     2041 ttacatcata ggcccgttgc gccacgatca tattgaccat ctcttcaacc acctgcacgt
     2101 tggacatttc caaggcgcct tgcacgattg caccgactcc ctctgtcccc ggggtggcat
     2161 tgaccggttc accggaagcg ctgctttgta ggaacaaatt ttttccttca ggaagtaaac
     2221 cagccgggtt cacgaaccgg accaattcga tctggccgat ctcctggggt aaagtttcac
     2281 ccggcatcat gacggtaacc gtcccatccg gtgcgacgct caaatcagtt gcgccgtccg
     2341 gaatggaaat gtcgggttgt aggtaatagc catcgttgtt caggagtctt ccttccccgt
     2401 cgttcttgaa gttaccgctt cgggtatagg ctattcggcc atccggcaac tgaacctgga
     2461 aaaatccatc accctcaatc attagatcca gcggatttcc cgtctgttgg acgtttcctt
     2521 gggtgaagag acggttcgtc gcggccaatc tggcacccaa acccacctga agaccggcgg
     2581 ggagttgtcc ggttcccgct tgagtcgagt ctccgggagc cctcaaagtc tgataaagca
     2641 agtcctcaaa ttcagcacgg attttcttga aaccgatcgt atttacgttc gccacattgt
     2701 tggatatggt atcgatattg gtttgctggg caatcattcc tgtcccggcg gtccaaaggg
     2761 aacgcatcat gttaagcgac ctccttaaaa taaaaaactg cctgagataa ctcttgggca
     2821 gtcgcttagt ccttgttccc gattggaacg aaaggcgcca tcatccgaaa gaaagatcgc
     2881 gcgcgtactt aatccttatc cgattcatcc atcgtcacgc atccacgtac attcaaattt
     2941 ttaacgtgtg gtgcgttcga tcatccttga aggaagatct ccatcggtca atcttccaaa
     3001 aacctccgca agcggtccgg tttcattttc ggatgaatct cttggacttt ggctttccgc
     3061 taagctttcc gcccgaactg atttaatttt tttaaacgcg tacttgtgta cgcgaattac
     3121 ctggccaatt cgttcacgag ttttcctaag gtatcgtcat gagccgtcag ggccttttga
     3181 ttggtttcat atccccgggt cgcggtaatc atctgaatca tctcgtaaac ggtattcaca
     3241 ttggaatgct ccaccgcacc ctggataacc tgaaagtctg tggccggcac cgggctttca
     3301 ttggtataga gatattcgcc ttccttctcc atgcctgcgg tagaataaac ctgcaacctg
     3361 tttacgatct ggccgttttg gatgatctcg ccgctttccg tgacttggaa cgcaccctcc
     3421 tgtaactgga ttggtccatt ctgtcctaaa accttcaaac cttcctgact taccagaaaa
     3481 cggtccttgt ccaccatgaa attaccggcc cgcatatacc ggatgccgtt ctgggcctgg
     3541 actgcaaaca tcccgtcgcc acgcatggcc agatc
//
LOCUS       DASVMB010000396         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_713162,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000396 DASVMB010000000
VERSION     DASVMB010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713162"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF531_11205"
     CDS             complement(<1..304)
                     /locus_tag="VF531_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714575.1"
                     /translation="MDKIRVLIVDDHNVVRDGLKTLIETRTTDIEVVGTAVNGQEALD
                     VVEKIQPDLVLMDLRLPQLNGVEATRLIRQNYPKIKVLILTSYDEEKFVVEGMQAGA"
     gene            complement(273..1517)
                     /locus_tag="VF531_11210"
     CDS             complement(273..1517)
                     /locus_tag="VF531_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7714576.1"
                     /translation="MDRFWAMIRWGASLGTSVLFGLVFFLSLTDLKTGSGVPLLVYSG
                     LIAAGAGWIGNLILVWIAVSVQKGKVPFPKWIGTVLSWSGTALLILLPGAHHTVLPWL
                     FAVTVGCAMLALTFRQNLIFVGLLGLSAIASDFWIRPRMDISVFLLILVIMIGAAGVI
                     HFLYRELTAARNEMSQLRREVGYLTQTNVRLQNHAVQEREMILGGERERIAREFHDTL
                     GHTLTGLVVKLQAIQSLVRLDPDRVIEEIDGSCALVREALREARLTVSALRDPVSQVI
                     RGSMLWLTLCETFAKCTGITIQTDFEEEFDTVDDVTNAVVYRFIQEALTNAYRHGEAT
                     LIDVAVWWRRNELRVRVSDNGHGTNGLQEGFGITGIRERVSELKGQVGWKSEYGKGFD
                     IAIIIPCSRGEASGQNTSVDCG"
     gene            complement(1652..>4057)
                     /locus_tag="VF531_11215"
     CDS             complement(1652..>4057)
                     /locus_tag="VF531_11215"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714577.1"
                     /translation="EGQEPKIAFFAGLPEKGTGLYSRIPLLAVPRLNDEAELNICRYT
                     VFSRCAAYYITEWADFLAAVRIFVNADHEMIFTILARNRTVKPQQIFLSSFLNPFLAH
                     DIVENAEHRWFREVSIIRPTGSPHELPSFMIKVNEDLSRTQSLSNYGIIRRKIVLGSH
                     CRMTGHQETASRYQYVGGIRGNLNDPSSLKNGGFGREKAVCAFTESGIAGDIIHLEIG
                     ADESGRLDTVFGYCFNAPDRERLLERPVDCDRIEGDLSRAEREELAGSHGLSMEIGPA
                     NQEWLRPRVWNAFFEHLKKQVEFCSLIKGYVQLATTSLIGIRDIFQALEGLLFWQPEV
                     CRAKILEALGFIFPDGRCPRQYSLPPENGGSPVMDLRPFIDQGAWVIASIITYLRVTG
                     DFGFLDESCGYYEIIDEKAKTVRKSNIQDNVLGHMLRIMDYLISNLDSKTHCLHALYG
                     DWNDALDGLGVSYDPEREYGSGVSVMASLQVYQNLLEMMELLQKIDPEEHAARISRYR
                     ATLNSLQAGLLEYAVITNGPQQRIVHGWGDDRRYLVGSFNDPDNQARDSLTANAFWVL
                     SKLYDIDPGIKPAILSAFERLDSKYGLRTFVPHFEMNTPGVGRITKLPPGTAENGAAY
                     IHASAFGIMALFRMGCPEKAWEQLRKSLPFTHQTISGSPFVMPNSYGLNEEKLIDGES
                     MADWQTGSSNVILKTLIRFVFGIEPQFDGVWIQPAVWMPFNSFRFRIMIRTTSLSIRY
                     QNQGGKRRTYRVNGENRGTVYDEVMRLEKLWLANGEFRGTELNIEVIDSSDSDITESH
                     PKR"
BASE COUNT         1061 a         1097 c          907 g          992 t
ORIGIN      
        1 tggcacccgc ctgcatcccc tcgaccacaa atttttcctc atcgtaactg gtgagaatca
       61 acaccttgat cttgggatag ttttgccgga tgagccgggt cgcttccacc ccgtttaatt
      121 gcggtaagcg taaatccatc aagaccaaat cgggttggat tttttccaca acatccaagg
      181 cctcttgccc gttaaccgcg gttccgacta cctcaatatc cgtcgtccgg gtctcaatca
      241 acgtttttaa tccatcacgc actacgttgt ggtcatccac aatcaacact cgtattttgt
      301 ccactggcct ctcccctgga gcatggtatg atgatggcaa tatcaaaccc tttaccatac
      361 tcgctcttcc aacccacctg tccttttaat tcggagaccc gttcgcgaat cccggtaatt
      421 ccgaacccct cctgtagtcc attcgttcca tgaccgttat cggaaacacg aacccgcagt
      481 tcgttccggc gccaccaaac cgcaacatca attaaagtgg cttctccgtg ccgataagca
      541 ttggttaaag cttcttgaat aaaacgatat acaacagcgt tggtcacatc atcaacggtg
      601 tcgaattcct cttcgaagtc ggtttgaatt gtaattccgg tacacttggc gaatgtttcg
      661 cacaaagtca gccaaagcat gcttccccgt ataacttgac tcacaggatc gcgtaaggcg
      721 gaaaccgtca ggcgtgcctc gcgaagggct tcccgaacca aagcacatga accgtcgatt
      781 tcctcgatga cccgatccgg atctaatctg accaatgatt gaatcgcctg taacttaact
      841 accagaccgg tcaaagtgtg ccccagagta tcgtgaaatt cacgggcgat ccgctcccgt
      901 tccccaccca aaatcatttc ccgttcttga acggcatgat tttgcaaccg gacattggtc
      961 tgggttaaat accccacttc acgccgtaat tgactcattt cattccgggc tgcagtgagt
     1021 tcacggtaca aaaaatgaat caccccagcc gccccgatca ttatcactag aatcaaaaga
     1081 aaaacggaga tatccatccg gggccgaatc cagaaatcgc ttgctatcgc ggataaaccc
     1141 aataacccaa caaagatcag gttttgccta aaggtcaatg ccagcatggc acagccaacc
     1201 gtaaccgcaa atagccatgg taaaactgta tggtgagcac ctggcaatag gattaacaac
     1261 gcggtaccgc tccaggataa aaccgtccca atccacttgg gaaaaggaac ctttcctttc
     1321 tgtaccgaaa cggcgatcca cactaaaatg aggttcccta tccatccggc accagctgca
     1381 attaagccgg aataaacgag caagggaacc cctgaacctg tttttaaatc cgtcaaactg
     1441 aggaaaaaca ccaatccaaa cagcacactg gtccccagtg aagctcccca gcgaatcatc
     1501 gcccagaaac gatccatttg gattcctcac tcttgatctg ataaatagat tttcgcgccg
     1561 agaccttgtt ttaccttcct aattcttatg ttttatcgaa aacaaccggt cattggcgtg
     1621 gatgcgcacg cgcaaaatta ctatcaattc attaacgttt tgggtgagat tcagttatat
     1681 ctgaatcact ggaatcgata acctcgatgt ttagttcagt acccctaaac tcgccgtttg
     1741 caagccacag tttttcaaga cgcatcacct cgtcataaac tgtgccgcga ttctcgccgt
     1801 tcactctata ggtgcgccgc ttcccgcctt ggttttgata tcggatcgaa agtgaagtgg
     1861 tacgaatcat gatccgaaac cggaatgagt tgaacggcat ccagactgcg ggttgaatcc
     1921 aaactccgtc aaattgcggc tcaataccga agacaaaccg gatcagcgtt ttgagaatca
     1981 cattggaact tccggtttgc caatccgcca tggactcacc gtcaatcagc ttttcctcgt
     2041 ttagaccata agaatttggc atcacaaaag gtgaaccgga tatggtttga tgggtgaaag
     2101 gcaatgattt tcgcagctgt tcccaggcct tttccggaca acccatccgg aataacgcca
     2161 taattccaaa ggctgaggcg tgaatataag cggcgccgtt ttccgcggta cctgggggta
     2221 acttagtaat ccggccgact cccggagtgt tcatctcaaa gtgcggcacg aaggttctta
     2281 agccgtattt ggagtccagc cgctcgaatg cggaaaggat ggcgggcttt atacccggat
     2341 cgatatcgta caattttgac agtacccaaa aggcgttggc agtcaaactg tcgcgggctt
     2401 gattgtcagg atcgttgaaa cttcctacca gatatcttcg atcatcaccc caaccgtgca
     2461 caatccgttg ctgcggtcca ttggtaatca ccgcatattc cagtaatcct gcctgtagtg
     2521 aatttaatgt cgcccggtaa cgggagattc tggcggcatg ctcttccggg tcgatttttt
     2581 ggaggagttc catcatctca agcagatttt gataaacctg taatgaggcc atcaccgata
     2641 caccggagcc gtactcccgc tcaggatcgt aactcactcc caatccatcg agggcatcgt
     2701 tccaatcacc atagagcgca tggaggcaat gagtctttga atcaaggttg gagatcaagt
     2761 aatccataat tcgcagcatg tgtcccagaa cgttatcctg tatgttgctt ttccttacag
     2821 ttttagcctt ttcgtcaatg atctcgtaat acccgcagct ttcatcgaga aatccaaaat
     2881 caccggtaac acggagatag gtgatgatgg atgcaatcac ccaagccccc tgatcgatga
     2941 aggggcggag atccatcacc ggtgaaccgc cattttcagg cggcaatgaa tattgacgcg
     3001 ggcaacggcc gtccgggaag ataaaaccga gtgcctccaa aatcttagcc cggcaaactt
     3061 cgggttgcca aaaaagcaac ccctccaagg cttgaaaaat atcacggatc ccgattagcg
     3121 aggtggtagc caattgaacg taacctttga tcagggaaca aaattcaact tgtttcttta
     3181 agtgttcaaa aaaagcgttc catacccgag gccgcaacca ttcctggttc gccggaccga
     3241 tttccatgct taatccgtga cttccggcca gttcctcccg ttctgcccgg gataaatcac
     3301 cttcaatccg gtcacaatcg actggccttt ccaatagacg ttcccggtcg ggcgcattga
     3361 agcaataccc gaataccgta tccagccggc cggattcgtc cgcgccgatc tccaggtgta
     3421 tgatgtcacc ggcaatgccg gattcggtaa aggcacaaac ggctttctcc cttccgaagc
     3481 cgccattttt caaggaggac ggatcattca ggttcccccg gattccgcct acatattgat
     3541 aacgggaagc ggtctcctga tgaccagtca ttctgcaatg agaacccagg acgattttgc
     3601 gccggatgat cccgtaattt gagagggatt gagttctgga gaggtcttca ttaaccttaa
     3661 tcataaagct tggcaattcg tggggggagc cggtcggccg gataatgctt acctccctga
     3721 accagcgatg ttcggcattt tcaacgatgt catgggcgag gaagggattg aggaaagatg
     3781 acagaaagat ctgttggggt ttgacagtcc ggttacgggc caaaatcgta aatatcatct
     3841 catgatcggc atttacgaag atccggaccg ccgccagaaa atccgcccat tccgtaatat
     3901 agtaggcggc gcacctcgaa aaaacagtat accggcagat attgagttcc gcctcgtcgt
     3961 taagccgggg tacagccaaa agtggtattc gtgaatagag tccggttcct ttttcgggaa
     4021 gaccggcaaa aaaggcaatc ttcggttcct gcccttc
//
LOCUS       DASVMB010000397         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_417746,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000397 DASVMB010000000
VERSION     DASVMB010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417746"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF531_11220"
     CDS             complement(<1..382)
                     /locus_tag="VF531_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX7714578.1"
                     /translation="MMKIILAPDSFKGTMSSIKICNIMSAAIRKYFYDAEIIKIPVAD
                     GGEGTVECFLEAVGGKKVAVRVKGPYFEELDSFYGRLADEKTAVIEMAAAAGLPLVEN
                     NKNPLLTTTYGVGQLIRHAVEGGCT"
     gene            923..2158
                     /gene="mef(A)"
                     /locus_tag="VF531_11225"
     CDS             923..2158
                     /gene="mef(A)"
                     /locus_tag="VF531_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEX7714579.1"
                     /translation="MTEQTSKWQRNFFTIWAGQAISLITSAILQMAIIFYLTEKTGSA
                     LVLSIASMVGFLPYALLGPAIGVLVDRWDRKRVMIGADLMIAAAGAALAVIALSMELP
                     VWLIMAVLCIRSIGSAFHSPALSAVTPLLVPKEKLTQCAGYSQSLQSISYILSPAVAA
                     FLYSIWELNAIIALDVAGAIIACITVALIPIPKLNVEKQSLKRNFMGEMKEGFGVIRA
                     NQGLFALLLVGTLYMFVYMPINALYPLISMKYFGGTAMHVSITEIAYASGMLVGGLVL
                     GLFGGYQKRITLITASILLMGVSLTVSGLLPANGFVLFVACCAIMGLSVPFYSAVQTA
                     LFQERIKPEYLGRVFSLTGSIISLAMPVGLILSGVFADQIGVHHWFLLSGLIITGIAA
                     LCPLVPEIRKLNRKENVEV"
     gene            2416..>2613
                     /locus_tag="VF531_11230"
     CDS             2416..>2613
                     /locus_tag="VF531_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7714580.1"
                     /translation="MYKISEFSKISGLTVKALRYYDEQDILKPSFRADNSYRYYDEED
                     FKRAGLIALLRNLDFSIPEIRD"
BASE COUNT          624 a          630 c          613 g          746 t
ORIGIN      
        1 tggtgcaacc gccctccacg gcatgtcgga tgagttgacc cacgccatag gtggttgtca
       61 ataacggatt cttgttattc tcaaccagtg gcagtccggc ggcggcagcc atttctatca
      121 ccgcagtctt ctcatctgcg agcctgccgt agaatgaatc cagttcctca aagtacggcc
      181 cctttacccg aactgccacc ttttttcctc cgaccgcttc caggaaacat tcgacagttc
      241 cctcaccgcc atcggccacc gggattttta tgatctccgc atcataaaaa tattttcgga
      301 tggcagcaga catgatatta caaatcttta tcgagctcat cgttccttta aatgaatcgg
      361 gggccagaat aatcttcatc acgaccatac ctccggtaag aaagaatgaa atataaagta
      421 tcccatttag tttatcatca tcgcgccgga tattacaagt ttccgtactg attattcatt
      481 cggaggcgat acttgtaatc attgtgcatt cacgctataa tgtttttgtc agctggttga
      541 ttcccctatt tcctttcgtt gatgtaaaaa acaaccattc tgcaaaaatt ttaaagagtg
      601 aagtggcaaa cacagccaat tagattttta gcttcacctt ttcaatttac actttagttc
      661 cggaggatac tgattccatg caccatattc gtttaagata agctggcaaa agagcgctaa
      721 gttaccctcc accaacagtg ggcttttttg tcatgcttat tttcacgaat cgggacatgg
      781 aacagggcac gtgtatcatt ctgaagttgc cccttgatac tcatttgtct ttggatcgtg
      841 gatatgcaga tcaggcccgc ctggcggagg atgatctgca tttttattgt ccaaaaacga
      901 tttgataaag gagcaaccga atatgacgga acaaacttca aaatggcaac ggaatttctt
      961 tacgatctgg gcgggacagg cgatttccct catcaccagc gctatcctgc aaatggcgat
     1021 cattttttac cttaccgaaa aaactggttc cgccttggta ttatccatcg cttcaatggt
     1081 gggattcctt ccctacgccc tgttgggccc ggccatcggg gtattggttg accgctggga
     1141 tcggaaacgg gtgatgatcg gcgcggattt aatgattgcc gcggccggtg cggccttggc
     1201 agtaatcgcc ttatccatgg aactgccggt ctggctgatc atggcggttt tatgcatccg
     1261 cagcatcggc agtgcctttc attcaccggc attgagtgcg gtgacacccc tgttggtacc
     1321 gaaagaaaaa ctgacccaat gcgccggcta cagccagtcg ctgcagtcaa ttagttatat
     1381 tctaagtcct gcggttgcgg catttttgta ttccatttgg gaacttaacg cgattatcgc
     1441 cctcgatgtg gcgggggcaa tcattgcctg tatcacagtg gcgctcattc ccattcccaa
     1501 actcaatgtg gaaaagcaaa gcctgaaacg aaatttcatg ggggaaatga aagaaggatt
     1561 tggggtcatc cgggccaacc aggggttatt tgccttgctg ctcgtgggca cgctctatat
     1621 gttcgtttat atgccaatca acgcccttta ccccctgatc agcatgaagt atttcggcgg
     1681 gacggcgatg catgtctcca ttacggaaat agcctacgcc tccgggatgt tggtgggcgg
     1741 gttagtattg ggactatttg ggggctatca aaaacgaatc actctgatca ccgcatctat
     1801 tttgttgatg ggcgtcagct tgaccgtttc gggcctgctt cctgccaatg gatttgtcct
     1861 attcgtcgcg tgctgtgcga tcatgggact ttcggtgccg ttctacagtg ctgtacaaac
     1921 ggccctgttt caggagcgga tcaagccgga atatctgggg cgtgtattct cgctgaccgg
     1981 cagcattatc tccctcgcca tgccggttgg gctgatcctc tcgggagtat tcgccgacca
     2041 aatcggtgtc catcattggt ttttattgtc cggtctgatc atcaccggca ttgcggccct
     2101 ttgtccgctg gttccggaga taaggaagct gaaccggaag gaaaatgtag aagtttaacg
     2161 agaagcttgt tactggatga tattttggat gaaagtattc ttaatttgac tacccggaga
     2221 gcggggtacc gctttccggg tatcatccta actttaagtg tttttgctcc ttaaaaagcg
     2281 aaacctctac tgccacccca tgtaaccttt ataacgtgtt ataatttaga aagtggcttt
     2341 catgtcattc actcaaatac ccattgacct tcccccgcga tggagggttt atacttaatt
     2401 caagcaggtg aggccgtgta taaaatcagt gaattttcaa agatcagcgg tttgaccgtt
     2461 aaggctttac ggtattatga cgagcaagac attttaaagc catccttccg ggccgataat
     2521 tcctatcgct attatgatga agaggatttt aaaagagcag gactcattgc tctgctgcgc
     2581 aacttagact tttccatccc ggaaatcagg gat
//
LOCUS       DASVMB010000398         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_713316,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000398 DASVMB010000000
VERSION     DASVMB010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713316"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="lpxD"
                     /locus_tag="VF531_11235"
     CDS             <1..597
                     /gene="lpxD"
                     /locus_tag="VF531_11235"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7714581.1"
                     /translation="GLGTIIHSNVVIREDCELGNNVQIHAGTVIGADGFGYVTVNGKH
                     FKVPQIGRVVIEDDVEIGSNVSVDRATTNVTLIKRGSKIDNQVQIAHNCQIGEDNMIC
                     GQAGIAGSTKIGDRVTLAGKAGVVGHIKVGDDSVVAASSIVISSLPANSFVSGFPARH
                     HAEDMRIQAAAGRLPELLKEFRELQKKVAELEGKIHDK"
     gene            893..1975
                     /locus_tag="VF531_11240"
     CDS             893..1975
                     /locus_tag="VF531_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7714582.1"
                     /translation="MRIIHRYIWKEYLQQLLLCLMGFTVLGIGKILFENNDLFIGYRM
                     SARLVWVLLLDQIPSLWMDVLPASVLFGVIITIGRMHRDRELDVIRVAGVNLLQLASP
                     ILIGVFGFCFLAFWWNDLVVPGANHNFQVEVRRLSMQENLPLLKENVIFKGPENRFIY
                     LSKVEHKAKRIEGVLIFETGAAGSWPRVITAKHGQIRQGVWVLFDGVIHDFDENGTVR
                     TELAYRRMEVKMSVDYSAMMGQEKGPSEMRTGELLQLIGLYKRSGMNYPVYSVYYHQK
                     FADPLISVVLAFLALPLSLLTGRNNRVFGLIWCFLIIMGYYTIQVIGRTMGCNGIILP
                     WIAAWAPHLLFLVVGLFVFTAIEHRG"
     gene            1978..>3098
                     /locus_tag="VF531_11245"
     CDS             1978..>3098
                     /locus_tag="VF531_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX7714583.1"
                     /translation="MKKPAVIGSLILIFLNLFANRAFAETVPASLTADEINYDYQLQQ
                     MEATGHVRLSYKDVVVESDQVIIDQEQDIVLALGNVSVTQKDSKFEGERFLYYLESDQ
                     GWVSPLKAEATDSQIKGPVQFTSEEAYIKGESIRTKKSTFTSCDLDQPHYHYTARAIE
                     YYPGDRIVFHHVWYWEANIPLVYLPWLFISLKKENENLEWEVGRNATEGWWLRLGYNY
                     YLNSASHGVLTTRVTEKGANEYGVKHYFDTSATSRWYGLFNLIDQSKLEHPRNDTMFG
                     VGFEDWSNVPFRLERSEMKSWERYLADGDPYRQHNLDLKFTGQSPYPNLTLHYADDSS
                     KVLNQYDISGNWAYNRSQTGLRMDMNGRWFYQTSAGTIP"
BASE COUNT          789 a          649 c          818 g          842 t
ORIGIN      
        1 ggtttgggta ctatcatcca ttctaatgtg gtaattcgtg aggattgcga gttgggaaat
       61 aatgttcaga tccatgccgg aacagttatt ggggccgatg gattcggata cgttactgtg
      121 aatggtaaac atttcaaagt gccgcagatt ggccgggtcg tcattgaaga tgatgttgag
      181 attggttcga atgtttcggt tgaccgtgcc accaccaatg taaccctgat taaacggggt
      241 tccaagattg acaatcaggt acaaatcgcc cacaactgcc agattggtga ggataacatg
      301 atctgcggcc aggcgggaat cgccggcagc acgaagatcg gggatcgagt gacattggcc
      361 ggtaaggccg gtgtggtcgg gcatatcaaa gtaggggatg attcggtggt agcggccagt
      421 tccatcgtaa tcagcagttt acccgccaat tcattcgttt cgggctttcc ggcacggcac
      481 catgctgagg atatgagaat tcaagctgcc gcgggacgat tgccggagtt actcaaagag
      541 ttccgggaac ttcagaaaaa agtagcggaa ttggaaggaa aaatccacga caaataatga
      601 gttttatata aacaaaaact ggataacggc aggaatttgt cgaaactgaa agaaatcatc
      661 cttcaaaaaa attacttcat tcaaagatga atttccgggc cgctatccgg tgatccgtat
      721 cactcaccgg tatggaatgt ataacctttc cgaacctcgg gaagtggagt tatccaagcg
      781 cctctggtgc ttttttttat gaagcatctc cgaagcggat tcggtaagaa tacgcattac
      841 ttaagtgatt tatggatggc tcaagagaca gctttaagac gaggtactat tcttgcggat
      901 tattcatcga tatatctgga aagagtatct acaacaattg ctcttgtgct tgatgggttt
      961 cacggtactc gggattggaa agattctctt tgaaaacaac gatcttttca tcgggtaccg
     1021 gatgtccgcc cggcttgttt gggtattgct ccttgaccaa atcccttctt tatggatgga
     1081 tgttctcccg gcctcagtac tattcggagt gattatcacc atcggccgga tgcatcggga
     1141 cagggaattg gatgtgatcc gggtagccgg tgtaaacctg ctccagctcg cgagcccaat
     1201 cctcatcggt gtatttggct tttgcttcct ggccttttgg tggaatgatt tggttgtccc
     1261 gggtgccaac cataatttcc aggttgaagt acggcgtttg tcgatgcagg aaaatcttcc
     1321 gttacttaag gaaaacgtta tttttaaggg accggagaac cggttcattt acttgagcaa
     1381 ggtagaacat aaagcgaaac ggattgaggg ggtactgatc ttcgagaccg gcgccgccgg
     1441 aagctggccc cgggtaatca ccgctaaaca tggtcaaatt aggcaaggcg tctgggtgct
     1501 gtttgatgga gtgattcatg attttgatga aaacgggacg gtccgtaccg aactggctta
     1561 ccgccggatg gaagtaaaga tgagcgtgga ttattcggcg atgatggggc aagaaaaagg
     1621 tcccagtgaa atgaggaccg gtgagttgct tcaattgatc ggtctataca aacggagcgg
     1681 tatgaattat ccggtttatt cggtttacta tcatcagaag ttcgccgatc cactcatctc
     1741 tgtggttttg gcttttttag cccttcccct gtcccttctc acaggcagga acaatcgggt
     1801 ttttggatta atctggtgtt ttctgattat catggggtat tacaccattc aagtcatcgg
     1861 gaggactatg gggtgtaatg gcatcatttt gccgtggatt gccgcgtggg ctccacactt
     1921 actatttctc gtcgtcggac tgtttgtgtt tactgccatt gagcaccggg gatagatatg
     1981 aaaaaaccgg cagtgatcgg aagtttgatc ctcatttttt taaatctatt tgcaaaccgg
     2041 gctttcgcgg aaaccgtccc ggcatcgctt accgcggatg agattaatta cgattatcag
     2101 ttacagcaga tggaggccac cggtcatgtc cgattgagct ataaggatgt ggtcgtcgaa
     2161 tccgatcagg taatcatcga tcaggaacaa gacattgtgt tggctctggg aaacgtgagt
     2221 gtcacccaga aagactccaa atttgaagga gagcggttcc tgtattatct tgaatcggac
     2281 caagggtggg tgtcaccgct caaggcggag gcgaccgact cccagatcaa gggaccggtg
     2341 caatttacct cagaggaagc ctatatcaaa ggtgaatcaa tccggaccaa gaaatcaact
     2401 tttacctcgt gcgatttgga ccaaccccat taccattata cggcacgggc catcgagtat
     2461 tatccggggg atcggatcgt attccaccat gtctggtatt gggaggcaaa catacccctg
     2521 gtttacctgc cttggttatt catctcttta aaaaaagaga atgaaaacct tgagtgggaa
     2581 gtgggccgga atgcaactga gggttggtgg ctgcgtttgg gttataatta ttacctaaac
     2641 agtgccagcc atggagtttt aaccacgcgc gtcacagaga aaggcgctaa tgagtatgga
     2701 gtcaagcatt attttgatac ttccgctacg agtcgctggt atggcctgtt caatctgatc
     2761 gatcagagta aactggaaca tccccgtaac gatacaatgt ttggtgtggg ttttgaggac
     2821 tggtccaatg ttccgtttcg gttagaacgt tcggagatga aatcctggga gcggtattta
     2881 gcggatggtg atccgtaccg gcagcataat ttagatttaa agtttaccgg tcaatcgcct
     2941 tatcctaatc tgacattaca ttacgccgat gattcatcga aggttttgaa tcaatatgat
     3001 atctccggca actgggccta taaccggtcc caaaccggat tacggatgga catgaatggt
     3061 cgttggtttt atcagacttc cgcagggaca atcccttt
//
LOCUS       DASVMB010000399         8261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_49973,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000399 DASVMB010000000
VERSION     DASVMB010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49973"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1491
                     /gene="nifK"
                     /locus_tag="VF531_11250"
     CDS             118..1491
                     /gene="nifK"
                     /locus_tag="VF531_11250"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HEX7714584.1"
                     /translation="MLLRHTPQEITEREALVVNPAKTCQPVGAMYAALGIHNCLPHSH
                     GSQGCCAYHRSALARHYKEPVMASTSSFTEGAAVFGGQANLIEAIQNLFTIYNPDVIA
                     VHTTCLSETIGDDIPQIIAKARDEGKIPSGKYVIHTNTPSYIGSHVTGYANMTKGMVN
                     YFATTTGVRKKQLNIIPGWVEPSDMREIKHLASNMGISIVMFPDTSDVLDAPQTGHHS
                     FYPKGGVTIPALKSTGDSIATVALGQYASAPAAEALDAKCRVRCEVLDLPIGLRATDR
                     FIDTLRQITEVPVPEAMNDERGRLVDIITDMQQYFYGKKVALCGDPDQLIPLSEFLAD
                     LGMKPVHIVTGTPGKHFEQRMAAILKDIPGARFRQAADMYLLHQWIKNERVDLLIGNT
                     YGKYIARDEDLPFIRFGFPILDRIGHSYFPYVGYQGGMRILEKILSALMDRQDRDTPE
                     EQFELVM"
     gene            1571..2956
                     /gene="nifE"
                     /locus_tag="VF531_11255"
     CDS             1571..2956
                     /gene="nifE"
                     /locus_tag="VF531_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HEX7714585.1"
                     /translation="MMQILEERKHQVFRKGSDRFEMECNKRSLAGAVSQRACVFCGSR
                     VILYPIADAIHLVHGPIGCAAYTWDIRGSLSSGPELHRLSFSTDLSELDIIQGGETKL
                     RRALRELIARYQPQAAFVYATCIVGLIGDDVAAVCAQISRETGIPVIAVESEGFKGTK
                     KDGYRAACEALLKLIGTGTETADPGRSSNGCEYTLNILGDFNLAGEVWLISDYYRRMG
                     IEVVATLTGDGRIDRIRAANRARLNLVQCAGSMIYLAQRMEELYGIPYQRVSYFGIED
                     MAESLYNAARFFGGSELLARTRELVREEIGAVYPRLLEYRKDLNGKKAAIYVGGGFKA
                     FSLVKALRLLGMKTVLVGTQTGTTEDYRQLRDLCDPDTVIVDDSNPLELAEFLTESDV
                     DLFIGGVKERPIAYKLGIGFCDHNHERKEALAGFSGMVNFAREVHSSVMSPVWRLAPR
                     RNRRSREESNG"
     gene            2996..4351
                     /locus_tag="VF531_11260"
                     /pseudo
     CDS             2996..4351
                     /locus_tag="VF531_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
     gene            4373..5635
                     /locus_tag="VF531_11265"
     CDS             4373..5635
                     /locus_tag="VF531_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7714586.1"
                     /translation="MTWNLGNHPCFNREAASLYGRIHLPVAPWCNIQCAFCNRSYDCA
                     NENRPGVTSAVLSPRQALHYLGQALQRDARILVVGIAGPGDPFANPAETMETLALVKE
                     NYPELGICLASNGLGLSPYVDALADSGVTHVTVTVNAVDPVIGAQIYAWVRDGRVVYR
                     GRDAAMLLFERQQDAIRRLKQKGLIVKVNTIVIPGVNDGQVPAVAERMRKLGVDRMNC
                     IPIYPARGTVFESIVAPGEEFMAELRQKLAKILPQMGHCARCRADASGLLAEPMTSVQ
                     LELLTASAALPLDPSESRPYVAVASMEGFLINRHLGEAEQLFIYRKSPSGFELVEKRP
                     TPEPGGGSLRWEQLAKVLNDCRAVLVSAAGASPREILKKQGIAVVEVEGLIDQALAAV
                     YEGKPLNSFKKFHRSACGGGCTGQGAGC"
     gene            5691..5996
                     /locus_tag="VF531_11270"
     CDS             5691..5996
                     /locus_tag="VF531_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX7714587.1"
                     /translation="MQRPVHHIMICSSSRFNGEPKGICRNKNAAELIQYLEEALSERG
                     MDQVLVTNTGCMKLCDRGPIMVIYPEGYWYGGVDQAAIDRILDALSTGGSVKELLLT"
     gene            6044..7171
                     /locus_tag="VF531_11275"
     CDS             6044..7171
                     /locus_tag="VF531_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEX7714588.1"
                     /translation="MAEVTGFPNPPRLIDSTLRDGEQAPGVAFSRNEKVIISCLLDQL
                     GLTELEVGIPAMGSEEVDLIREIASLELNCKLTGWCRARRDDIDRAAQAGLERVHLSF
                     PVSESHLGAIGKDRKWLFSSLKPLIEYARESFGFIAVGALDGSRTDRIVLEDFACMAF
                     EYGADRLRIADTVGILNPMQTWELIHGLHELIPDLELEFHGHNDLGMATANTISALSA
                     GAGSASVTVNGLGERAGNAPLEEVAMAMQITLKMDPGIRTVNLAGVCSKVARFAGRPI
                     PAAKPLTGAMVFTHESGIHCHGLLKDPHTYQPFLPEQIGRTGSRLVIGKHSGSAVLRH
                     LLKEEGIERNDDEIAVLLQRVRSLAMVKKGSLSMDELRMLL"
     gene            7203..7973
                     /gene="modA"
                     /locus_tag="VF531_11280"
     CDS             7203..7973
                     /gene="modA"
                     /locus_tag="VF531_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7714589.1"
                     /translation="MKCLKALFLVLLSLFPVITLNAAPAQPVAVVVSAAASLTDLMNE
                     AKIAFEAEHSGVRIDLNFAATGVLQTQIEQGAPVAVFAGASSDNLAALNKKGLLDEKL
                     MKVLCGNTLTLLLRPGLTLPGAGSFAQVTDPLLKRIAVGDPQYVPAGKYAITLLTKLN
                     LAESVRPKLVYASNVREALTWVGLGEVDAAIVYNTDAITAKGLQVQATTKPDELKIEY
                     GIAVTKIGSQIPGSYQFVAFLTGDQGRKLLKKYGFLTD"
     gene            7987..>8261
                     /locus_tag="VF531_11285"
     CDS             7987..>8261
                     /locus_tag="VF531_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX7714590.1"
                     /translation="MDPGFFHSIMAAAVISIRTAALATVFAFICGGALAWFCSGRRSW
                     AIALLEGAISLPLVLPPTVLGFYLLLVFGRGSWPGRLICAMMNTDII"
BASE COUNT         1857 a         2040 c         2441 g         1923 t
ORIGIN      
        1 cgccccgtgg caaaaagaac cggaacttac cgcaactttg gccgtagatg aataaagaac
       61 ccggtttata cgtgcataag cgaaccggct agaaatgagt ttcaggagga atttaaaatg
      121 cttctcagac atacgccgca ggaaataacg gagcgtgagg cgctggtagt caacccggca
      181 aaaacctgtc aaccggtggg tgccatgtac gccgcgctgg ggatccataa ctgcctgcct
      241 cacagtcatg gttcccaagg atgttgcgcc tatcatcgca gcgccctggc gcggcattac
      301 aaggaaccgg tcatggcctc caccagttcg ttcacggagg gggcggcggt atttggcggc
      361 caggctaatt taatagaggc gattcagaat ctgttcacca tctataatcc ggatgtgatt
      421 gccgttcata ccacctgtct ttcggagacc atcggggacg atattccgca gattatcgcc
      481 aaagcgcgtg atgaaggcaa aatcccatcg ggcaagtatg tgattcatac caatacgcca
      541 agttatatcg gatctcatgt gaccgggtat gccaatatga ccaagggaat ggtgaactat
      601 ttcgccacca ccaccggagt gcgcaagaaa caattgaata tcattcccgg ctgggtggaa
      661 ccttccgaca tgcgtgagat aaaacatctg gcaagcaaca tggggatttc catcgtgatg
      721 ttcccggata cttccgatgt gctcgacgct ccccagaccg ggcatcattc gttttatccc
      781 aaagggggtg ttaccatccc cgccttaaaa agtaccgggg acagtatcgc cactgtggct
      841 ctgggccagt atgcatccgc tccggcggcc gaggcgctgg atgccaagtg ccgggtgcgt
      901 tgcgaggttt tagatctgcc catcggatta agggcgactg atcgtttcat cgatacgctc
      961 cgtcaaatca ccgaagtccc ggttccggag gcgatgaacg atgagcgcgg ccggctggtc
     1021 gatatcatca ccgatatgca acagtatttc tacggcaaga aagtggcgct ttgcggcgat
     1081 cccgaccagt tgattccact ctccgaattc ctggcggacc tggggatgaa gccggttcat
     1141 atcgtcaccg ggactcccgg aaagcatttt gagcaaagaa tggccgcgat tctcaaagat
     1201 attcccggag cccgtttccg gcaggccgcg gatatgtatc tgttgcatca atggatcaag
     1261 aatgaacggg tcgatctgtt aatcggaaat acttacggaa aatacatcgc ccgggacgag
     1321 gatctgccgt ttatccggtt cggttttccc attctggatc ggatcggtca ttcgtacttt
     1381 ccttatgtgg ggtaccaggg cggcatgcgg atacttgaaa agattctttc cgctttgatg
     1441 gatcgccagg atcgggacac tcctgaagaa cagtttgaat tggttatgta acctttttgg
     1501 atttggcgac ttctgatacg gtacggttta tggtccaatc ccgcgtgcgg gtcatcaggg
     1561 aaggtgaagc atgatgcaga ttcttgagga acggaaacac caggtctttc ggaaaggcag
     1621 tgaccgcttt gagatggaat gcaacaagcg gagcctggcc ggagcggtca gtcagcgggc
     1681 ttgcgtcttc tgcggttcgc gggtgatcct gtacccgata gcggatgcca ttcatctggt
     1741 gcacggtccg atcggctgcg ccgcatatac gtgggatatc cgcggttcgc tctcatccgg
     1801 tccggaattg cacaggctta gtttttccac ggatctgagc gagcttgata tcattcaagg
     1861 cggcgaaacc aaactgcgcc gggcgctccg ggaattgatc gccagatatc agccgcaagc
     1921 ggcctttgtc tacgccacct gcatcgtggg gttgatcggc gacgatgttg cggcggtttg
     1981 tgcgcaaata agccgggaga ccgggattcc ggtcattgca gtggagtccg aaggtttcaa
     2041 gggaaccaag aaagacggtt accgggcagc ttgtgaggca ttgttgaagt tgatcggcac
     2101 cggaaccgaa acggccgatc cgggccgatc ctcaaacggg tgcgaataca ccctgaacat
     2161 cctgggcgat ttcaatcttg ccggggaagt ttggttaatc agcgattatt accggcggat
     2221 ggggatcgag gttgtagcca cgcttaccgg agatgggcgg atcgaccgga tccgcgccgc
     2281 caaccgggca cggttgaatc tggtgcaatg cgccggatcc atgatttatc tggcgcaaag
     2341 gatggaagaa ctttacggta taccgtatca gcgggtttca tacttcggga ttgaggatat
     2401 ggcggaatcc ctttacaatg cagcccggtt tttcggcgga tctgagttgc tggcaaggac
     2461 ccgggaactg gtccgggagg agatcggggc cgtatacccc cggttattgg aataccgaaa
     2521 ggatctgaac ggcaaaaaag ccgcgatata cgtgggcggt ggttttaaag ctttttcgct
     2581 ggttaaggcc ttgaggctct tggggatgaa aacggtattg gtcggcactc agaccggaac
     2641 taccgaagat taccggcaat tgcgcgatct atgcgatccg gacaccgtta tcgtcgacga
     2701 ctccaaccca ctggaactgg cggaatttct aaccgaaagt gacgtggacc tgtttatcgg
     2761 aggcgtcaag gaacggccca tcgcttataa gctgggaatc ggtttctgcg atcataatca
     2821 tgaacggaaa gaagccctgg cggggttcag cggtatggtg aatttcgccc gggaggttca
     2881 ctcatccgtc atgagtccgg tttggcgtct ggcaccccgc cgtaatcgcc ggagccggga
     2941 ggagagcaat ggttaggtat ttcacagttc aatctttata aaggggggaa gcgggatgat
     3001 cgtaacccat caggccgagt ccgccaaagc ggtgacgatt aatgcctgta agctttgttc
     3061 accgctggga gcctgtttgg cttttcgggg cattgccaat tcaatgacgg ttttgcatgg
     3121 atcccaaggc tgcgccacct atatccggcg ttacatcatc agtcatttca aagagcccat
     3181 ggatatcgcc tcctcgagtt tcaatgagtc gaccgcgatc ttcggaggaa aagataactt
     3241 gctgaccgcc atccggaatg ttgctgcgca atatcatccg gaagtgatcg gggtggccac
     3301 gacttgcctg agtgagacca tcggtgatga tatcggatcc tacatccgcg agtattccaa
     3361 aaccgattcc cgccaccccg agcttgtggc ggtgacgact ccggggtacc agggaacgca
     3421 ccgtgacggt tttcaggaag cgatcaaggc cgtttgcggt ttggccggca ccggaacccc
     3481 catcgaccag gttaatctct tcccgggatt cgtttccccg gccgatctgc gatatctaaa
     3541 ggagattcta aatgatttcg gtctggcctt cgtgatgctc cccgattact ccgaaaccct
     3601 ggatggtccg atctggtcgg agtatatggc cattcccccc ggcggtacgc cgctttccct
     3661 gattcagagc atggggcggt ccaaggctag catcgaattg ggacggatca tcaatcccaa
     3721 aaattccgca gcccaaagcc tgaatgagcg gtgtagggtt cccggatata gcatagggat
     3781 gtcggtggga gtccgggaga ccgacaactt atttgaagta ctaacacaac tcagcgggca
     3841 tccaataccg gataaatacc gtcaggaacg ggagcgccta atcgattcct atatagatgg
     3901 gcataaatac ctcttcggca agaaggccgt gatatacggt gaagcggatc tcgtcgtagg
     3961 tctggccgga ttcctggcgg agatcggcgt cattccgata ctttgagctt ccggggaaga
     4021 gcatggcagg ctggcggcgg cattggcgga agttgcaccg gatatgagct ctccaatgca
     4081 tgcctatgaa ggtgccgact tttcagccat aaccatccaa gccgagaagg ccaagccgga
     4141 tctgatcatc ggtaacagca aaggctatgc aatggccagg caactgaagg tgccgctggt
     4201 ccgggtgggg tttccggtgc atgatcggat cgacggtgcc cggattttac atctgggata
     4261 tcgcggtacc cagcaattgt tcgaccggat tgtcaatgga ttgatggaac agcagcagaa
     4321 cctgtccccg gtcggctaca gttatatctg aatcaaagcg agcgtgagag agatgacttg
     4381 gaatcttgga aaccacccct gttttaatcg ggaagccgcg agtctctacg gccggatcca
     4441 tctgccggta gcaccgtggt gtaacatcca gtgtgctttt tgcaatcgca gttatgactg
     4501 tgccaatgaa aaccggcccg gagtaaccag tgccgtgctg tctcccaggc aggccctgca
     4561 ctacctcggt caagcgctgc aacgggatgc gcgaatcctg gttgtaggga tcgccggacc
     4621 cggcgatccc ttcgccaatc ccgcagaaac catggagacc ctcgccttgg tcaaggaaaa
     4681 ttatccggaa ctcggaattt gcctggcttc caatggatta ggactttcgc cgtacgtgga
     4741 tgccttagcg gatagcggtg tgacccatgt gaccgtaacg gttaatgcgg tggatccggt
     4801 aatcggcgct caaatctatg cctgggtacg ggacggccgg gttgtatacc ggggccggga
     4861 cgctgcaatg ttattgttcg agaggcaaca ggatgcgatt cgccgtttga aacagaaggg
     4921 tctgatcgtg aaggtcaata cgatcgtcat tcccggtgtc aatgacgggc aggttccggc
     4981 agtagccgaa aggatgcgca aattgggagt ggaccggatg aactgcatcc cgatttatcc
     5041 cgcccgggga acggtatttg aatcgatagt cgctccgggt gaagaattta tggctgaact
     5101 ccgccagaaa ctggcgaaga ttctgccgca gatgggccat tgtgcacgct gccgggccga
     5161 tgcatctgga cttctggcgg agcccatgac ttccgtgcaa ctggaactgt tgacggctag
     5221 tgccgctttg ccgttagacc cctcggagag ccgtccttat gttgcggtgg caagcatgga
     5281 aggattctta atcaaccggc atctggggga ggcggaacaa ttattcattt accggaaaag
     5341 tccgtcaggt tttgagttgg tggagaaacg gccgaccccg gaaccgggcg gtggatcatt
     5401 acgttgggag caattggcca aagtgttgaa cgattgccgg gcagtactgg taagcgccgc
     5461 cggagcttca ccccgggaaa tccttaaaaa acagggtatc gcagtggtag aggttgaggg
     5521 actgattgat caggcattgg ctgctgttta cgaaggaaaa cccctgaata gttttaaaaa
     5581 atttcaccgt tccgcttgcg gcggaggttg taccgggcag ggtgcgggat gttgaattgg
     5641 accttggact tttgattttg acttgaaatt gaagggaggg aaaatgtgtc atgcaaaggc
     5701 ccgtacatca tatcatgatc tgttccagct cccgttttaa tggggaaccc aaaggaatct
     5761 gcagaaataa aaatgcggcg gaactgattc aataccttga ggaggcacta tccgaacggg
     5821 gaatggatca ggtgctggtc accaataccg ggtgcatgaa actctgcgac cgcggcccga
     5881 tcatggtaat ctatccggaa ggatactggt atggcggggt tgatcaggcg gctatcgacc
     5941 ggattttgga tgcgctatcg accggcggct ccgttaagga actgctgtta acatgagaca
     6001 gccggttgct tctaatgccg gatagaagtt gggaggcatg cttatggctg aagtaacggg
     6061 ttttccaaat cccccgcggc tcatcgacag tactttacgt gacggcgagc aagccccggg
     6121 ggtcgccttc tcccgtaatg aaaaggtcat catcagctgt ctgcttgacc agttgggtct
     6181 caccgagctg gaggtcggca ttccggccat gggatccgaa gaggttgatc tgatccggga
     6241 aatcgcctcg cttgagctga attgtaaact gacaggatgg tgccgggcga ggcgggacga
     6301 cattgaccgg gccgctcagg cgggtctgga acgggtgcat ttatcctttc ccgtctcgga
     6361 gagccatttg ggagcgattg gcaaagaccg gaagtggttg ttttccagtc tgaagccttt
     6421 gatcgagtat gcccgggaat cattcgggtt tattgccgtc ggagcgctcg acggctcccg
     6481 caccgaccgg atagtattgg aggacttcgc ctgtatggct ttcgagtacg gcgctgacag
     6541 gctccgcatc gccgatacgg tgggaatatt aaatcccatg cagacctggg aactcattca
     6601 tggtctgcat gagctcattc ccgatctgga gttggagttt cacggtcata acgatctggg
     6661 catggctacc gccaacacca tctccgcttt gagtgccggc gccggcagcg ccagtgtcac
     6721 cgtaaacggt ttgggcgagc gggccggtaa cgcaccgtta gaagaggtgg cgatggcgat
     6781 gcaaatcaca ttgaagatgg atccggggat ccgtaccgtc aatctggcag gggtctgcag
     6841 taaagtggcg cgatttgccg ggaggccgat tcccgcggcg aagccgctga ccggcgcgat
     6901 ggtttttacc catgagtctg ggattcactg ccatggattg ttaaaagatc cccatactta
     6961 ccagccgttt cttccggaac agatcgggcg gacgggatcc cgtctggtta ttgggaagca
     7021 ttcgggtagc gcggttctaa ggcatcttct caaagaagag gggatagagc gaaacgatga
     7081 tgagatcgcc gtattactgc agagggtaag aagcctggca atggtcaaaa aaggttcgct
     7141 ctccatggat gaactccgga tgttgcttta gggaatgaca aatttggggg tggagaaata
     7201 tcatgaaatg tttgaaagct ttgtttctgg ttttgcttag tttgtttcct gttataaccc
     7261 tgaatgcggc gccggcccag cctgtagcgg tggtggtctc ggctgcggcc agtctgacgg
     7321 acttgatgaa tgaggccaaa attgcatttg aggccgaaca ctccggcgtc agaatcgatc
     7381 taaatttcgc agcgaccgga gtcttgcaga cccagatcga gcaaggcgct ccggttgcgg
     7441 tttttgccgg tgccagttcc gataatctgg cggctttgaa caaaaaaggg ttgctcgatg
     7501 agaagttaat gaaagtgtta tgcggcaata ctttgactct cctgttacgg ccgggtctca
     7561 cacttcccgg cgccggatcc tttgcgcaag tgaccgatcc gctgctaaaa cgaattgcgg
     7621 tgggggatcc gcaatatgtt ccggcgggta aatatgcgat caccttgctt accaagttga
     7681 atctggcgga atcggtccgg cccaaactgg tttatgcctc caatgtacgc gaagctctaa
     7741 cctgggtcgg cctgggagag gtcgatgcgg ccattgttta caatacggac gcgatcaccg
     7801 ccaagggctt gcaggtgcaa gctacgacca aacccgacga gctcaagatc gaatacggga
     7861 ttgcggttac caaaatcggc tcccaaattc ccgggtccta tcaatttgtc gcttttttga
     7921 cgggagatca aggccggaaa ttactgaaga agtatggctt tttgaccgat tagggaggaa
     7981 agcggtatgg acccgggctt ttttcatagt attatggctg ctgcagttat atcaatccgg
     8041 accgctgctc tggctacggt ttttgctttc atctgcggcg gagcactggc atggttctgc
     8101 tccggccgtc gttcctgggc aattgccctt ttggaggggg ccatttcgct tcccctggtc
     8161 ctgccgccga cagttttggg gttttatcta ttattggttt tcggccgggg tagttggccc
     8221 ggtaggctta tctgcgccat gatgaatacg gatatcattt t
//
LOCUS       DASVMB010000400         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_713647,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000400 DASVMB010000000
VERSION     DASVMB010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713647"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..645)
                     /gene="dut"
                     /locus_tag="VF531_11290"
     CDS             complement(184..645)
                     /gene="dut"
                     /locus_tag="VF531_11290"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7714591.1"
                     /translation="MNTKITVKCKVISPLMESGRIPIPAYATPGAAALDLHACCPEPL
                     LIPAGSRGKVPTGIAIELPPDTVALVFPRSGLASKHGISLSNAVGVIDSDYRGEIICL
                     VKNESDTDFTVHPGDRIAQLAFFPVYRGSLETVPELDESERGEGGFGSTGI"
     gene            complement(635..1897)
                     /locus_tag="VF531_11295"
     CDS             complement(635..1897)
                     /locus_tag="VF531_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7714592.1"
                     /translation="MYQRATLPNGMRIICETIPQVHSVSLGVWVGTGSRYEDISYNGI
                     SHFIEHMIFKGTSRRSARDIAETIDAVGGQLNAFTTKEYTCFYAKVLDRHFELGVDLL
                     ADMVTSSRFATEEIEKEKQVILEEIKSYEDAPDELIHDILADTILKGHPLGWTILGTP
                     ESVDRIDQSAIISYLKQHYTPDNIVFAAAGNVQFDQIVAAVAERFASLGGLVRQELPP
                     VPPVQPTATVRFKDIEQVHLCLGARGLGRQDPDKYVVFVLDSILGGSVSSRLFQQLRE
                     EEGLVYVTGTNHASYKDTGIFSIYAATGIEHFEKVMGLIKKELHQIQQVLVEPVELAR
                     AKDQLKGNLLLSLESTCNRMSRIGKTELFHDQLLTPEESVARIDKVSSEALLRVASRL
                     FAEDRMVLTALGPFPKNSKVFTSSYHEY"
     gene            2278..>2498
                     /locus_tag="VF531_11300"
     CDS             2278..>2498
                     /locus_tag="VF531_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714593.1"
                     /translation="MQGNLLTTILALVFYIASGLFGGNSNSNIPVPAGSDTAYTNPAS
                     GFPRSGVVSGAQVNLRNSYLGSGTVIDTI"
BASE COUNT          620 a          657 c          576 g          645 t
ORIGIN      
        1 agaaaacaga gcgtagtttt ataaatcaat acgcgaagcg gcagcctgtt cagactgccg
       61 ctttgttata ctttttttcg gttctgtttt ttatctcctt accccgggca ataattcgtt
      121 attcactaga tttacaggat aatacgggga gtaagtttac aaaaccgtac atcaacatta
      181 ctatcatatc ccagtggaac cgaacccgcc ctcgccgcgc tccgattcgt caagctccgg
      241 gacggtctcc aagcttcccc ggtatacagg gaaaaaggca agttgggcaa tgcgatcccc
      301 gggatggacc gtaaaatccg tgtcgctttc gttcttgacc aggcagatga tctccccccg
      361 ataatcgctg tcgataacac ccacagcgtt agaaagggaa atcccgtgtt ttgaggccaa
      421 gccactgcgg gggaaaacca atgccaccgt atccgggggt aattcaatcg caattcccgt
      481 aggaacctta ccccgggacc cggcagggat caacagcggc tccgggcaac aggcgtgaag
      541 atccaatgcc gccgcgcccg gagtggcata tgccggtatc ggaatccggc cgctctccat
      601 caagggagag atcactttac attttacggt tatcttagta ttcatgataa ctactcgtga
      661 aaactttaga gtttttggga aaaggtccca aagcggtcag aaccatccga tcttccgcga
      721 aaagtctgga cgccacccgc aacaaagcct cactactcac tttatcgatc cgggctacac
      781 tctcctccgg ggtcaggagt tgatcgtgga acagttcggt cttgccgatc cggctcatcc
      841 ggttgcaggt gctttccaga ctcaaaagca gattcccttt aagctggtct ttggcccggg
      901 ccagttccac cggttctacc aatacctgtt gaatctggtg caactccttt tttatcaaac
      961 ccatgacttt ctcaaaatgt tcaatgccgg tggcggcgta aattgaaaaa atcccggtat
     1021 ccttataaga agcgtgattg gtaccggtta cgtaaaccag gccctcttcc tcccggagtt
     1081 gttgaaagag ccgggaactg acgctccccc ccaatatgct gtcgagtacg aaaaccacat
     1141 atttatccgg atcctgtcgg ccgaggccac gggcgcccaa gcaaagatgg acttgttcaa
     1201 tatccttgaa cctgactgtt gccgtgggtt gaaccggcgg taccgggggt aactcctgcc
     1261 tgacaagtcc cccgagactt gcaaagcgtt ctgccacggc tgccacgatc tggtcgaatt
     1321 gcacatttcc cgctgctgca aaaacgatat tgtctggggt ataatgttgc tttagataac
     1381 ttataattgc ggactgatcg atccggtcga cgctctcggg ggtacccagg atggtccatc
     1441 ccagcggatg gcctttgagg attgtatcgg ccaatatatc atgaatcaat tcatccggcg
     1501 catcctcata agatttaatc tcctccagga tgacttgttt ctctttttcg atctcttccg
     1561 tggcgaaacg ggaactcgtg accatatccg ccagaagatc cacacccaat tcgaagtgcc
     1621 ggtcaagcac cttcgcgtag aaacaagtgt attccttagt ggtaaaggca ttcaactggc
     1681 cgcctaccgc gtcgatggtc tcggcgatat cccgggcact acggcgggag gtgcctttga
     1741 aaatcatatg ctcaataaaa tgggaaatcc cgttatagct tatatcctca taacgggaac
     1801 cggtgccgac ccaaacacca agtgataccg aatgtacctg gggtatggtc tcacagatga
     1861 ttcgcattcc atttggcaat gtggcgcgtt ggtacatagg ttttccttta attgatgtga
     1921 tatttaacat aaaacttcgc tctcatttcc ggaaatcctt ctttagccaa gagtgattaa
     1981 aggagatgcg cattcgcaga tgatattccg tttttacctt attattcaca cttcggagat
     2041 gcatcccctt tttggtggga tgttcttaaa ggtcttgcct gttttgagga gaatcatcct
     2101 atttaaagcg cggcatcttt ctgtttgaac cgattcaagg atatcagggc atcaaaccta
     2161 tttatatact ttacatcgtg aaagccatac caataagctt catgaggacc ccctctgaaa
     2221 aaaccattca aagggtcttt agcagcagta attaatttga gctgaaagga gcccccgatg
     2281 caaggcaatc tcttaaccac aatactcgca ttggtatttt atattgcatc cggtttattc
     2341 ggcggcaact ccaattcaaa tataccggta ccggccggtt ccgacaccgc ctacaccaac
     2401 cccgcctcgg gatttccccg gtccggagtg gtttccggtg cgcaagtcaa tctacgtaac
     2461 agctatctgg ggagcggcac cgtgattgat actatcaa
//
LOCUS       DASVMB010000401        12120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_640992,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000401 DASVMB010000000
VERSION     DASVMB010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12120
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640992"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..742)
                     /locus_tag="VF531_11305"
     CDS             complement(56..742)
                     /locus_tag="VF531_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7714594.1"
                     /translation="MIKLARRIDDSEFVKEYDIKEHKFMTMEQEKRERVIDAALNEFS
                     KGYLAANTDVIVKEAGISKGLLFHYFGSKRGLFLFLIKYTLDIFFTEYEKVISESHDL
                     LENIRIGSKLKMELSFRYPLIFGFLLKAYSAIKEVFPEGLPGDLPYSSHMVDVMRQIC
                     RRSNNEKTLFKEGIDSEKAQNIIIWTMNGFIDSLLRYGDDVDDYRAHYDEMVKELDEY
                     LQILRKILYQ"
     gene            complement(933..1859)
                     /locus_tag="VF531_11310"
     CDS             complement(933..1859)
                     /locus_tag="VF531_11310"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7714595.1"
                     /translation="MRQQTFTVEPEHQGERLDQYLSATGVWPSRSFVQKLIAAGGVMF
                     NQPLGNDRPLKPGQRLEQGMRIVVAWDDPQPLTVAAEAIPLEILYEDHDLIVINKPRG
                     MVVHPAAGNYHGTLVNALLNHCHDLSGIGGVIRPGIVHRLDKDTSGVLVVAKNDLAHL
                     DLAAQIKARTMKRIYRVLIHGAPPEEGRIDAPIGRHPVERKKMAVVPGGRHAVTNYRV
                     LEYLGAYSYLEARLETGRTHQIRVHLSFLGYPLVGDTVYGRTREQVPLVGQALHAATL
                     GFIHPRSREYLEFTTEIPEVMRVALEWCRKNG"
     gene            complement(1834..2607)
                     /gene="lgt"
                     /locus_tag="VF531_11315"
     CDS             complement(1834..2607)
                     /gene="lgt"
                     /locus_tag="VF531_11315"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7714596.1"
                     /translation="MTLFRIFGLSIHAYGLMLAIAFLVAIFAIAKVGKPLGIEFDTII
                     DLATWVIIGAVIGARIAYVLTNLPYFLSSPWWEVFMVSSGGLAFHGGLIGGFGAGFWF
                     VKRRGIYPWRLADLVAPFIALGYSIVRIGCFLNHCCYGKVSNLPWALPCGPDLAARHP
                     TQLYSFLGSLILFGILWRFRNHKQFPGFLFLLYLGLYSIMRFIVEIFREGPMIFPWLT
                     EAQLVCIILGGAAFGLIGWFRRGAQNGRPGTDATADLHR"
     gene            complement(2690..3133)
                     /gene="lspA"
                     /locus_tag="VF531_11320"
     CDS             complement(2690..3133)
                     /gene="lspA"
                     /locus_tag="VF531_11320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7714597.1"
                     /translation="MPYILLTVAVIILDQGIKWLIQTRMQLFESYPIWDGMVSLTYAG
                     NNGAAFNILQGRSKLLALIAVVVFLVIWFKRRDIARYPRFFKVGLALALGGAVGNFID
                     RVRLGFVVDYVDFHIWPVFNLADAAILCGAALIILGFLRNDVKDN"
     gene            complement(3300..3959)
                     /locus_tag="VF531_11325"
     CDS             complement(3300..3959)
                     /locus_tag="VF531_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX7714598.1"
                     /translation="MDSEKLQRFRERLIEEKQRIEAELARSRSFNLEEPLTESVQELS
                     GYDNHPADLGSETFEREKDLGLRNNTAEIYQRIGEALERVDNGCYGICEDCGREIDLE
                     RLTALPYTTLCVDCQREEEDHHQTRHRPIEEEALMPPFGRTFLDNTDNVATDGEDIWQ
                     SVARYGTSDSPSDLSGVPNYGDLFADAGEKQGVVEDVEAVIDVGPDDIPPDPGNPDQK
                     D"
     gene            complement(3978..4277)
                     /locus_tag="VF531_11330"
     CDS             complement(3978..4277)
                     /locus_tag="VF531_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HEX7714599.1"
                     /translation="MLNRRMAKALFRKMYEVEGQLERANLAEFMQLLERPVRLLWLNF
                     IAGLARGFGIAIGLTIIASLFIVLLSTLAKLNLPIIGKYIADLVRIVNQQLRLYS"
     gene            4494..5243
                     /gene="gpmA"
                     /locus_tag="VF531_11335"
     CDS             4494..5243
                     /gene="gpmA"
                     /locus_tag="VF531_11335"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX7714600.1"
                     /translation="MTKLVLLRHGESTWNRDNRFTGWTDVPLSAVGEREARKAGQILF
                     ANGYRFDQAYTSLLKRAIKTLDLVLEELDQSWIPVFKSWCLNERHYGALQGMDKAEIA
                     AKYGAEQVHIWRRSFTVRPPELEPSDPRFPGRDPRYQMLNPDQLPLAESLEDTSARLL
                     PYWRETIVPAIREGRYVLIAAHGNSLRALVMHLDGISPAIISDLNIPTGIPLVYELES
                     NLKPIRHYYLEEPADIQADSGVKSDSGLTRI"
     gene            5356..5526
                     /locus_tag="VF531_11340"
     CDS             5356..5526
                     /locus_tag="VF531_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714601.1"
                     /translation="MYIIGYNRTNQFYFISKEEQRARLEASGVQVLTEEFAHITSAAA
                     ALRELNHETSKR"
     gene            complement(5754..7085)
                     /locus_tag="VF531_11345"
     CDS             complement(5754..7085)
                     /locus_tag="VF531_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7714602.1"
                     /translation="MSKRSGIFFVCCLVLVFGLSFTVYGTSKPKVLKLSINHAVNDAP
                     VELVIQGGKFYKNTFVKLTKAGQPEILASEVRLISTSEISCTLDLQGRAVGSWDLIVA
                     NIGNITKKVKPTVLPNGFTIEYPAPIVAGVAPNRSENNTTVLLQVNGTGFRPGSNVVL
                     RNGQTEIKGVVKSTSNQRIEAEFDLTGAVPGQYDLEIQNEDSKTGLFAKGFTVVAPAQ
                     PGALEIRSLTPNQGVNTGKIMVEITGANFSDKISVKLSGNGRPEITGLNVKAASPVKL
                     SCFFNIQDQPAGKYDVVLSDPSGRSVSLPDGFTVVAETRPAGKQLQPIFFGFDQTGLR
                     PDQMENLESNVKALQENPEWYVILGGHADERGSEEYNLGLTARRGQAVKDFLIGRGIA
                     AERIIIYAYGESYPAQDGHDEAAWRYNRRVDFLEWNTILTREQVLSETKLD"
     gene            7636..8580
                     /locus_tag="VF531_11350"
     CDS             7636..8580
                     /locus_tag="VF531_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEX7714603.1"
                     /translation="MGGRDQFQDFCTGFREELDSLARALPDSTPRPAFIWLEANACSG
                     DSISMLNTVQPNLGQLLCKMVSVIYWSALMPDQGTAAVDNLMATAASGDFILAVEGAI
                     AVGGRGRFAVPFRIDGYNFTSLELIRWLAPKARYIIAVGTCASFGGPSAARPNPSDSR
                     GVWEVVSEPVINVPGCPVNPDWFTGTLYHLIRFGPPELDRYRRPTLFYGETIHAHCQR
                     RSYFDNGIFAKKLGDPECMYNLGCMGPVTGSDCPYRLWNNHLNWPVKASTPCIGCTKS
                     GFPDRSTPFLTPFPEKLPRAGRGAGERSSRCPRSRSVR"
     gene            8553..9956
                     /locus_tag="VF531_11355"
     CDS             8553..9956
                     /locus_tag="VF531_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX7714604.1"
                     /translation="MPKITISPITRISGLLSIDIMLDSNRVVQEANCSGEQFRGFELM
                     LKGRQPEDAVYFTERICGICSMAHGYTSTRLVRKILGLEPTPEIVLLEQCLLGAEFLQ
                     NHIRHFYLLALPDYLPAAALPRINQGLSPETAPGFDVNVSQAQQHMLLEHYFRAMEAS
                     RKCHEMLAVFGGKIPHQHGMTAAGVAVIPTASRQVQFRSLLHQVQDFIHEAFLPDLHF
                     IAERYPDYETIGAGPNRFLCFGLFDPRYGGHFPAGIMVDGRPQTINPRAIREDIRYSW
                     YQETTENPLPEKPGAYSWVKAPRYQGLAFEGGPLARKVIASGLTGTTIAGTIPRLAAR
                     AEEAALIASWMEEWMNYLPQTGRYTAQPTGVPRSDAIQINDAPRGPLLHAMMVSGGTI
                     GRYTVITPSTWNFSPKDDHGERGPAEAALVGTVLADIKHPLEVGRIIRAFDPCLSCAT
                     HFIARQGENQIHFDLEV"
     gene            9973..10215
                     /locus_tag="VF531_11360"
     CDS             9973..10215
                     /locus_tag="VF531_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HEX7714605.1"
                     /translation="MSGGETPSRNFTRAELARFNGQNGQPSYVAVNGTVFDLTTVFIQ
                     GKHFMHLAGQDLTGSFLREHAPVSLSGYPVVGRLVD"
     gene            10453..11838
                     /locus_tag="VF531_11365"
     CDS             10453..11838
                     /locus_tag="VF531_11365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX7714606.1"
                     /translation="MFQKLNIRSMGKSGKDWTQKLSTRLINPEFSGRPEKPLRVIIEH
                     SGRKTDRITSLIENNQGKVNREIGLFPGLAVELPYSVLEELAHSRHVHKIWHDAPVRI
                     LLDVAVPTVGGTQVQQAGFTGTGVNVAVLDTGIYPHPDLVTPSNRILAWNDLVNHKEN
                     PYDDNGHGTHVAGIIAGNGYKSDGKYTGMAPETRLIGIKVLDQDGEGVISTIISGIEW
                     CINNFATLKLRAINLSMGSAAQESYRSDPLCRAATAAWRKGIVVCCAGGNEGPDSGTI
                     DSPGINPKTITVGNLDDQQTLSPSDDRLSDTSSRGPTIDRLMKPDILAPGTNITSTWK
                     NGGYRSLSGTSMSTPVVTGAVAQIVQKWPKLKPDQIKRQLLKNARDIGLGTNLQGAGV
                     LDLERIFKLTPNKAQSGAGFEADHLRQVCAYQIMTSLMDKAGKEKEPFLRKRDSSIQN
                     ALLSFMSKWFG"
BASE COUNT         3055 a         3276 c         2846 g         2943 t
ORIGIN      
        1 tgtgtagctt ttctttaagt ttgtaacatc aatgaacata ttcattttcc tccttttatt
       61 gatacaggat tttacgaaga atttgcagat actcatcaag ctcttttacc atttcatcat
      121 aatgcgcccg gtagtcatca acgtcgtcgc cataccggag caggctgtca atgaagccat
      181 tcatcgtcca tataatgatg ttctgcgcct tttcactgtc aatgccttcc ttgaacagtg
      241 ttttctcgtt attagatctc cggcaaattt gccgcattac atcaaccata tgggacgaat
      301 acggcaaatc gcccggtagc ccctccggaa agacctcttt tattgccgaa tatgctttca
      361 acagaaaccc aaatataagc gggtagcgaa aggagagctc catcttcagt tttgagccaa
      421 tcctgatatt ttcaagaagg tcatggcttt ctgaaatgac cttttcatac tccgtaaaaa
      481 aaatatccag agtatatttg atgagaaaca ggaaaagtcc cctttttgac ccaaaataat
      541 gaaatagcag tccttttgat atccccgcct cttttacaat aacatccgta tttgctgcca
      601 gataaccttt tgaaaattca ttcaacgccg catcaataac gcgctcgcgt ttttcttgtt
      661 ccatcgtcat aaacttatgt tccttaatgt catactcttt gacaaactcg gaatcatcaa
      721 tccttctggc cagtttaatc acctcattta aaagtccgtt gactacaagt caatatatgg
      781 attttagtat agcactccgt tgacttaaag tcaatgctgc tttaagcaat ttgttatccc
      841 catgggtaga ataaaaggat aatgctaaag cataacaaaa aaatgaagtc cgaaaggact
      901 tcactgtata cgatccatca gtttattaaa ttttatccat tcttgcggca ccactcaagc
      961 gccacacgca tcacttccgg aatctcagtg gtaaattcaa gatactcccg gctccgtgga
     1021 tggatgaatc ccagtgtggc ggcatgaagg gcttggccca ccagaggcac ctgttccctt
     1081 gttcgtccgt aaacagtatc acccaccaga gggtatccca agaaacttag gtgtacccgg
     1141 atctgatgag ttctcccggt ttccaggcgt gcttcaagat acgaataagc tcccagatac
     1201 tccaagaccc gatagttggt cacagcatgc cgtccgccgg gtacgaccgc catttttttc
     1261 cgttccaccg ggtgcctgcc gatgggggcg tcgattcgcc cttcttccgg cggagcgccg
     1321 tggatcagta cccgatagat tcgtttcata gtccgggctt tgatctgtgc cgccaggtcc
     1381 aggtgggcca gatcattctt ggcaactacc agtactcccg aagtatcctt gtccaaccgg
     1441 tggacgattc cggggcggat cacaccgccg attcctgaaa gatcatgaca atgattgagc
     1501 agcgcattta ccagcgtacc gtgataatta ccggcagccg gatggaccac catcccccgg
     1561 ggcttattga tcacaatcag atcgtgatcc tcataaagga tctcaagcgg aatggcttct
     1621 gccgcaacag tcagcggctg gggatcatcc caggcgacaa cgattctcat accctgttcc
     1681 agacgctgac cgggtttgag aggcctgtca ttccctaacg gttggttaaa cataacaccg
     1741 ccggcggcga ttaatttctg cacgaaagaa cgggatggcc agacgccggt agcggataga
     1801 tactgatcga gccgttcccc ttggtgttcc ggttcaacgg tgaaggtctg ctgtcgcatc
     1861 cgtaccgggc ctcccgtttt gggcgccacg ccggaaccat ccgatcaaac cgaaagccgc
     1921 gccccccagg ataatgcata ccagttgcgc ctcggtcaac cacgggaaga tcatcggacc
     1981 ctcccggaag atttctacga taaaacgcat aatcgaataa agccccagat aaagcaggaa
     2041 tagaaaaccg gggaactgtt tatggttacg gaaacgccac aggatcccaa atagaatcag
     2101 gctacccagg aacgagtaaa gctgggtggg atggcgggct gccagatccg gcccgcaagg
     2161 caaagcccag ggcagattgg agaccttacc ataacagcaa tgattaagaa aacaaccgat
     2221 ccggacaatg gagtaaccca gggcaatgaa gggtgcgact aaatccgcca gtctccaggg
     2281 atagatcccc ctccgtttga caaaccaaaa gccggcaccg aatccgccga tcaaaccgcc
     2341 gtggaaagca aggcccccgg agctcaccat aaatacctcc caccaggggg aactcaaaaa
     2401 ataaggtaaa ttggtcaaaa cataagcgat cctggccccg atcaccgccc cgataatcac
     2461 ccaggtggcc aaatcgatga tcgtgtcaaa ttcgattccc aatggttttc cgaccttggc
     2521 gattgcgaag atggcaacca aaaaggcgat ggccagcatt aaaccatagg cgtggattga
     2581 aagaccgaaa atccggaata aagtcatagc ggttcctcct tgaaaaaacc tgcgacagga
     2641 tatgggactg tcccaaatca aacagcggat aaccgtcccc tattcgtctc taattatctt
     2701 ttacatcgtt tcttaaaaat cccagaatga ttagggccgc tccgcacaga atggccgcat
     2761 ccgccagatt aaagaccggc caaatatgga aatccacata atcaacgaca aatcccaacc
     2821 ggacccggtc tataaaattt cctaccgctc cgccaagagc caatgccaat ccgactttga
     2881 aaaaccgtgg atatctggcg atatcgcgcc gtttaaacca gataaccagg aaaaccacaa
     2941 ccgcaatcaa ggcgagcaac ttcgaccgcc cttggaggat attgaaagcg gctccgttat
     3001 ttcccgcgta tgtcaatgaa accatcccat cccagatggg gtagctctca aataactgca
     3061 tccgggtttg gataagccat ttgatccctt gatccaagat gattacggct acagtaagta
     3121 aaatatacgg catggcattt gccccctacc aaaaaaccga cttttgccca aaagattccg
     3181 gcatttctca aggactattc gccatcctgt ctgaaaaacc ttttccagtt cctatcgtcc
     3241 gctgcccaaa ctcatctatt ggatcccagc gcttaagtga cgttatgacc tcaacagtct
     3301 cagtcttttt gatccggatt tccgggatcc ggcgggatat catccggccc tacgtcgatc
     3361 accgcctcga catcttccac cacgccttgc ttttcaccgg catcagcaaa aagatcacca
     3421 taatttggaa caccgctaag atcggagggg ctgtcggaag tgccatacct ggccacagac
     3481 tgccagatat cctcaccatc cgtcgccaca ttatccgtat tatccaaaaa agttctcccg
     3541 aacggcggca ttaatgcttc ttcctcgatc ggacgatgtc tggtttgatg gtgatcctcc
     3601 tcttcgcgtt ggcagtcgac acacaacgtg gtatagggca aggccgtcag ccgctcaaga
     3661 tcaatctccc gcccgcaatc ctcgcagatt ccgtaacatc cgttatcgac ccgttccaag
     3721 gcctccccga tccgttggta aatctcggcg gtattatttc gcaagcctaa gtccttctcc
     3781 ctctcaaagg tctcgcttcc cagatccgcc gggtggttgt cgtaacccga aagttcctgg
     3841 actgattcgg tcaaaggctc ttccagattg aaactgcgcg aacgggccaa ttccgcctcg
     3901 attcgttgtt tctcctcaat caaacgctcc ctgaaccgtt gcaatttctc tgaatccatt
     3961 tctccctctc cctttattta cgaatataac cgcagttgtt ggttgacgat tcgcaccaga
     4021 tcggcgatat actttccgat aatcggcaga tttaatttag ccagcgtgga aagaaggacg
     4081 ataaaaaggc tggcgatgat ggtgagccct atggcgattc caaagccacg ggctaaaccg
     4141 gcaatgaagt tcagccataa caacctgacc ggtctttcca gtaattgcat gaactcggct
     4201 aggttggccc gttccagttg accctcgacc tcatacattt ttcgaaaaag tgcctttgcc
     4261 attctccggt taagcatcga ccacacctgg tttggtttga aatttgcctg tgggacctgt
     4321 cggcccttag gttacctcgg tttcggtaaa aataatcacc accggattat tcctgttttc
     4381 cggataaatg ttgtataatt tttaccatga tgtatccacc cgtatttgaa tcttatgaaa
     4441 ccataatata ataaaaagat gagatctttc atttacccca aaggaggatc cccatgacca
     4501 aactggtact attacgacac ggcgaaagca cctggaatcg ggacaaccgc tttaccggct
     4561 ggaccgatgt acccttatca gccgttggag agcgggaggc aagaaaagcc ggtcaaatcc
     4621 tattcgctaa cggctaccgg tttgatcaag cctatacctc gctcctcaaa cgggctatca
     4681 aaacccttga cctggtactg gaggagttgg atcaaagctg gataccggta ttcaagtcct
     4741 ggtgtctaaa tgaacgccat tacggagccc ttcaggggat ggacaaagcc gagattgctg
     4801 caaagtatgg cgcggaacag gtccacatct ggcggcgcag ctttacggta cgacctccgg
     4861 agctggaacc atccgacccc cgtttccccg ggcgcgatcc ccgttatcaa atgctgaacc
     4921 cggatcagtt gcccctcgcg gaaagcctgg aggatacttc ggcccggtta ttgccctact
     4981 ggcgggagac aattgtcccc gctatccgtg agggccggta cgtcttaatc gcagcccatg
     5041 gaaacagctt gcgagcactg gtgatgcacc tggacgggat ctcacccgcg atcatctccg
     5101 acctcaacat acccaccggg attccactcg tttatgaatt ggaatcaaac ctgaaaccaa
     5161 ttcgccacta ttacctggaa gaaccggccg atatccaagc ggactccgga gtaaaaagtg
     5221 attccggact tacccgtatt tagatcaagg atcatcattg ccaatcggcc attaaatatg
     5281 gtataataaa aaaaacgaac aaatgttcgc aaattctgtg acatcttcta catttttaat
     5341 atggaggaga acacaatgta cattattgga tataaccgca ccaaccaatt ttacttcatc
     5401 agtaaggaag agcaacgggc caggcttgaa gcctccgggg ttcaggtact gacggaggag
     5461 ttcgctcaca tcacctccgc cgctgctgca ctgagggaac tgaatcatga gacctcaaaa
     5521 cggtagtagc ctggtttggc gcctatgact cactcttgct ctctctggcg acgggcgcca
     5581 aagtcgcgca catgtgcgcg cgcaacctca atccttcgca gtctatttta ttgctttgaa
     5641 tccgccttct cccctctttc ccgcttgccg ggagagaggg gccgggggtg agtcgcggaa
     5701 gagcaagact aaagcaaggg gaaatgctcc ccttgctttt tttaaccgaa atatcaatct
     5761 agttttgttt cagacagaac ctgctcccgc gtgagaatcg tattccactc caagaaatca
     5821 acacgccggt tgtatcgcca agccgcctcg tcatggccgt cctgggcggg gtaactctcg
     5881 ccgtacgcat agataatgat tcgttctgcc gctattccac ggccaataag aaaatctttg
     5941 actgcctggc cccgtctggc agttaaaccc agattgtact cctcggaacc gcgctcatcg
     6001 gcatgacccc caagaattac gtaccattcg ggattttcct gtaatgcctt cacattgctt
     6061 tccagattct ccatttgatc cggacgtaac ccggtttgat cgaaaccaaa aaatatcggt
     6121 tgcaactgtt taccggcagg gcgagtctcc gcaaccaccg taaatccatc cggcaaactc
     6181 accgaccggc cggaaggatc actcaaaaca acgtcgtact ttccggcggg ttgatcctga
     6241 atattgaaaa aacaactcaa cttgacgggt gaagccgcct tgacattaag gccggttatc
     6301 tccggccggc cgttgccgga gagtttaacg gatattttat cagaaaaatt tgcacccgta
     6361 atctctacca tgatttttcc ggtattgacg ccttgatttg gggtgagcga cctaatctca
     6421 agggcacccg gttgggcagg ggctacgact gtaaacccct tggcgaatag gccagttttg
     6481 ctgtcctcgt tttgaatctc cagatcatac tgacccggaa cggccccggt cagatcaaac
     6541 tccgcctcga tccgttgatt ggatgtggac ttgactacac ccttgatctc cgtttggccg
     6601 ttgcgtaaaa cgacgttgga tcccggacga aagcctgtcc cgttcacctg gagaagcacg
     6661 gtggtattgt tttcactccg gttaggagcg accccggcca caatcggtgc cggatattca
     6721 atggtaaacc cgttcggtag aacggttggc ttgacttttt tggtgatatt gccgatattg
     6781 gcaacaatca gatcccatga acccactgcg cgaccttgta ggtccaaggt gcagctgatt
     6841 tcggatgttg agatcaaccg gacttcactg gccaagatct cgggttggcc tgccttggta
     6901 agcttaacga atgtattttt ataaaacttt ccaccctgaa tcaccagttc caccggagca
     6961 tcattgacgg cgtgattgat ggagagctta agtaccttag gtttactggt gccatagact
     7021 gtgaagctta atccgaaaac taaaacaaga cagcatacaa aaaaaatacc agatcgttta
     7081 ctcatggtta ttcttccttt cgtattttag tggttgcttg aagccctatc cctcctccca
     7141 cttgaatgcc tgctctcccg gcctcacctc cttaagtgaa tttagcccga ctccttttga
     7201 atcgcctccc ctaaggaaag aactttcggg taacaaaaaa actccgcatt ccatggatat
     7261 cccacttcaa tacagagtct cactgaacct tcgtcaacaa agccgggtgc attcgcgcgc
     7321 ccatgcgcgc cagacaccgt cctgacgact ttgttcctgt tttcgatgaa ctttaaacag
     7381 acagttactc cctctgtagt acgcttactt aaatttttga taaaattaaa gaacctgaga
     7441 gtttaatcaa tattcgcgac gataaatatc tctaatatag tttttagaca aaacccggct
     7501 gattcctgct tcaaattaag ttaaattcag ttttgacatt tacggcctgc acatgttctt
     7561 agtagttgac cctccgatta atatcatccc cccgcctctc atataatgta gaaacctttg
     7621 gcattggagg taccgatggg gggaagggat caatttcaag atttctgtac cggattccgg
     7681 gaagaactcg attcattggc ccgggcttta ccggactcaa ctccccggcc tgcctttatc
     7741 tggctcgaag ccaacgcttg ttccggggat tccatctcca tgcttaatac ggtccagccg
     7801 aatctggggc aattactctg caaaatggta agtgtcatct attggagcgc cttgatgccg
     7861 gatcagggaa cggctgcggt ggataatctt atggccaccg cggcatccgg cgattttatc
     7921 ctggcggtcg agggtgcaat tgccgttggt ggccgtggcc ggttcgcggt tccgttccgg
     7981 atcgacggct ataacttcac cagtttggaa ctcatccgct ggctcgcacc aaaggcccgt
     8041 tatattattg ccgtcggtac ttgcgcctcc tttggcggcc cttccgcggc ccgtcccaat
     8101 cccagcgaca gccggggagt ctgggaagtt gtctccgaac cggtgatcaa tgtccccggt
     8161 tgtcctgtca accccgattg gttcaccggc accctatacc acctgatacg ctttggtccc
     8221 ccggaactcg accgttaccg ccggcctacc ctgttttatg gagagaccat ccatgcccac
     8281 tgtcagcggc gttcttactt cgataatgga atattcgcca aaaaactcgg cgatcccgaa
     8341 tgtatgtaca acttaggctg tatgggaccg gttacgggga gcgattgtcc ctacagactc
     8401 tggaataatc acttgaactg gccggtcaag gccagtactc cctgcatcgg ctgtaccaaa
     8461 tcaggatttc ccgaccgttc aaccccattt ctgactccgt ttccggaaaa attgccgcga
     8521 gccggacggg gcgcgggtga aaggagttca agatgcccaa gatcacgatc agtccgataa
     8581 cccggattag tgggctactc agcatcgata tcatgcttga ttccaaccgg gtagtgcagg
     8641 aagccaattg ctccggtgaa caattccgtg gctttgaatt gatgctcaag gggagacagc
     8701 ccgaagacgc cgtttacttt accgagcgaa tctgcggaat ttgttcgatg gcccacggct
     8761 atacctccac ccggctggtc cggaagatcc tggggcttga gccgactccg gagatagtac
     8821 ttttggaaca gtgccttctc ggagcggaat ttttacagaa tcacatccgc cacttttact
     8881 tactggcctt gcccgattat cttcctgccg cagccttacc ccggatcaac cagggacttt
     8941 ctccggaaac cgcccccggg tttgatgtaa atgtgagcca agcccagcaa cacatgctgt
     9001 tagaacatta tttccgggct atggaggcca gccggaaatg ccatgagatg ctggcggtct
     9061 ttggaggaaa gatcccccat caacatggta tgaccgcggc tggagtcgct gtaatcccta
     9121 ccgcctcccg acaagtccag tttcgctccc tcctccatca ggtgcaggat tttatccatg
     9181 aggcattctt gccggatctt catttcatcg ccgagagata tccggattat gaaaccatcg
     9241 gcgccggacc gaaccgcttc ctctgtttcg gtttattcga tccccgttat ggagggcatt
     9301 tccccgccgg catcatggtt gacggccggc ctcaaaccat caatccccga gcgatccgtg
     9361 aggatatccg ttattcatgg taccaggaga ccacggaaaa tccgttgccg gagaaacccg
     9421 gcgcatatag ctgggtcaaa gcgccgcgat atcaaggtct ggcctttgaa ggcggaccgc
     9481 tggccagaaa ggtaatcgcc tccgggctga caggaacgac aatcgccgga acgattcccc
     9541 gcctggcggc ccgggctgag gaagcggcct tgattgcttc atggatggag gaatggatga
     9601 attatctacc ccaaacaggt agatatactg cccaaccgac cggggttccc cggagtgatg
     9661 ccattcaaat caacgatgcg cctcgagggc ccctgctcca tgcgatgatg gtctcaggcg
     9721 ggacgattgg ccgctatacg gtgattaccc caagtacctg gaatttttca cccaaggatg
     9781 accacgggga acgcggcccg gcggaagcgg ctttggtcgg aacagtattg gccgatataa
     9841 aacatcctct ggaggtaggc cggatcattc gagcctttga tccctgtctc tcctgcgcta
     9901 cacatttcat cgcccggcaa ggcgaaaacc aaattcactt cgatctggaa gtctaggaaa
     9961 ggtgggtact gaatgtctgg cggagaaacc ccctcccgga actttacccg ggcagaactc
    10021 gcccgcttta acggtcaaaa cgggcaaccg tcctatgtgg cagtcaacgg tacggtgttc
    10081 gatctcacca ctgtcttcat tcagggcaaa cacttcatgc atctggcagg gcaggatctg
    10141 accggttctt tcctgcgtga acacgcgccg gtttcactct cgggttatcc ggtggttgga
    10201 agactggttg actaggcatg aggcatagtg agcatcgtat tcacctcccc caagtgtttg
    10261 taccttctat cacagcagat ggcatttttc cttgaaacgg aagatgactt ttggtatcat
    10321 ttgccattcg atatcatcct gagtagggca tcgcttcgtc gaagcatgaa taaaaatcgt
    10381 gcctattagc cagaaatcac cggccgatgc tgcatataat ggtgtagatc cataaatatt
    10441 ggtggtgatt gcatgttcca aaagcttaac atccggagta tgggtaaatc gggaaaggac
    10501 tggactcaaa agctgtcgac aagacttatc aatccggaat tcagcggacg tccggaaaaa
    10561 cccctccggg taatcatcga acactcgggg agaaaaacgg atcgcatcac ctcactcatc
    10621 gaaaacaacc agggaaaagt caatcgcgag atcggcttgt ttcccggttt ggcagtggaa
    10681 ttgccctatt cagtccttga agagttggcg cactcacgcc atgtacataa aatatggcac
    10741 gatgcaccgg tccggatttt attggatgta gccgttccta ccgtaggggg tacgcaggta
    10801 caacaagcgg gttttacggg aacaggtgtt aacgtggcgg ttttggatac cgggatctat
    10861 cctcatcccg atttggtaac accttcaaac cgcatcttgg cctggaacga tctggtgaat
    10921 cacaaggaaa acccgtatga tgataacggc cacggcaccc atgtcgccgg gatcatcgcc
    10981 ggaaacggat ataagtccga cggaaaatac accggcatgg cgccggaaac gcgtttgatc
    11041 ggtatcaaag tacttgatca agatggcgaa ggtgtgatct caaccatcat ctccggtatc
    11101 gagtggtgca ttaataactt cgccacctta aaactaaggg cgatcaacct ctcgatgggt
    11161 tctgcggccc aagagtcata ccgttccgat cctctatgtc gtgccgccac agctgcctgg
    11221 cgcaaaggga tcgtggtctg ctgcgccggg gggaatgaag gaccggactc cggcaccatc
    11281 gattcacccg gaatcaatcc aaaaaccatc accgtcggga accttgatga tcagcagaca
    11341 ctctcgccgt cggatgatcg cttaagcgat acctcaagcc gcgggccaac gattgatcgt
    11401 ttgatgaagc ctgatatttt ggctccggga accaatatca cttcgacatg gaaaaatggg
    11461 gggtatcgtt ccctttccgg tacctcaatg tccaccccgg tggttacggg ggctgtggcc
    11521 cagatcgttc aaaaatggcc caagcttaag ccggatcaaa tcaaacggca acttttgaaa
    11581 aacgcccggg atatcggact gggcacgaat cttcaaggag ccggggttct ggacctggaa
    11641 cgaatcttta agcttacccc caataaagcc caatccggtg ccggttttga agcggatcat
    11701 ctccggcagg tctgtgctta tcaaataatg acctcattaa tggataaggc cgggaaggag
    11761 aaagagccgt ttttgagaaa aagagattcc tccattcaaa acgctctatt atcattcatg
    11821 agtaaatggt tcggctagac tcgggtccga attgagggta caggttagtt tcatttatac
    11881 caaatgcctc gataaatgtt accttggtcc ggttaacccg gaataaaacg aaaatattat
    11941 attacagtag agcgctggtg gagcagagac ttctcccatc actggcgcgc tgccggatag
    12001 ggttcaggtt tgagcagacc ccacccgatt tctgaaaact atcacggatt tatacacgag
    12061 gaggtatcat catgggaacc ggtttaactg gtgctttcgg cacgaatgag atgttagcct
//
LOCUS       DASVMB010000402         7331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_419288,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000402 DASVMB010000000
VERSION     DASVMB010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419288"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VF531_11370"
     CDS             complement(<1..335)
                     /locus_tag="VF531_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714607.1"
                     /translation="MAYPNELEPGSAGGYAEVEMLRTHRKRRVSFWARVRDYRWLLLM
                     ILPAVLLQLAFSYVPMAGMLMAFRNVDEFHLPFGTKWVGFANFQFLTDIYFWQTVRNT
                     LVIAGTKFV"
     gene            complement(404..1414)
                     /locus_tag="VF531_11375"
     CDS             complement(404..1414)
                     /locus_tag="VF531_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714608.1"
                     /translation="MNYTIHDVAKKAGVSIATVSRVLNGKDRVKEETRKKIISVIEEL
                     NYVTNFSATTLRKNRTNVIGVIVPELTATFYAEVIKGIENKANESGVRMVVCDAGNNL
                     EKERDSVRFLYDRSVDGMIFIMPKMTDEELIRIKCDRRPIVLFGRNMQQHNIPTITVD
                     NRFGVYAAIRHFYSHGFMKIAYIGGIEENHLPDRRERLEGYKQAIIDCGWELRPEYIE
                     NGLYYEEAAGSAFARLMKLPDPPDAVFCGNDEMALGVLKAARKMGFKIPEQIGLIGFD
                     NIRICQYTSPTLTTVSQPAYNIGVLCCEKLIHSINNLDTEVVYSNLLIQPELVVRESC
                     GC"
     gene            complement(1708..3054)
                     /locus_tag="VF531_11380"
     CDS             complement(1708..3054)
                     /locus_tag="VF531_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7714609.1"
                     /translation="MDTAQQLGTGSVARLLWNFSLPAIVGMVVSALYNIVDRIFVGHG
                     VGLLGISATTVAFPISIIILAFGLLIGIGAAATISIKLGQGKHVEAEQILGNALISSI
                     LISIFVTVLGLAFEEPLLRAFGASSEVLPLAKEFVTIIIAGTVLQLIGLALNNIIRAE
                     GNPRLAMVTMLIGAVLNAIFNPFFIFVLHLGIRGSALATIVSSTVSSAWVVAYFIGKK
                     SLLKLKFENLRLRWTVVGQILAIGMAPFLFQLVASIITVAFNLSLESYGGDVAIAAMG
                     IINSVSMLILMPVIGINQGAQPIIGYNYGAGQLARVKKTLELAILGATAVTAGGFILV
                     QLFPGAIMSIFAGPDKALLEIGIPGLRIFLMMLPIIGFQVVSSNYYQAIGKATASILL
                     TLSRQVIFLLPALLILPRFFKVPGIWAAGPVADFLASILTAVFILRELHRIQEVGR"
     gene            complement(3389..4606)
                     /locus_tag="VF531_11385"
     CDS             complement(3389..4606)
                     /locus_tag="VF531_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714610.1"
                     /translation="MKISFTSGIFVLTQRVTLGLIGVVIWLELIPAGICRGAELAWSG
                     SLESSETYRSFRDPATSPPETFFDNNLNLKLDINGSEKVSGRVLLQYYTNPPSWLQVG
                     TAGSQSVFINQAYIDYSLTQDSLLRVGRQKISWGSGMAWNPTNYIGADKNRADLTTGF
                     PGVDAIDYQFNRGDFSATLALKPMESWEQWGRALKVQWAIPHCDLAFSLFQEGSANAF
                     GTDVAATVGEYTVYSELALRTGSQFYVSGSGETPALQTRSVDRHYLHGVLGMNRIFAD
                     NLMMQLEYYYNEEGWGIPEEKAYFNYVAEYPGERAALSAQKANLFGELGRNYLYLLIR
                     KTGILEDLSLTGNCLWNIEDHSYQLTPVLEYQLTQEAVTVLMVNFSNGDEASEFGSEL
                     SQYQILAKLVLNF"
     gene            complement(4618..5382)
                     /locus_tag="VF531_11390"
     CDS             complement(4618..5382)
                     /locus_tag="VF531_11390"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7714611.1"
                     /translation="MFKNHLLRVALIGMIVAGIMQAGASQTFAELNGDQIVARVDEII
                     GSSTGRYIADITVVRPGKDERTSRVQVYIKGSEKVLVRYLAPAQEKGQGYLRIGDDEW
                     LFLPSANKSLRVSGRQNMQGSDLANDDILRIKLTTDYTAQLLGTESIAGVNHYLLELT
                     ARQPSVAYGKLKFWVRQEDFIPTRTEYYATSGKLIKIMTYNQIQMIGGKTRPTMMEIQ
                     SELRKGYKTIFHIVEADFTGENPDQLFTRLNLEKGK"
     gene            complement(5415..6662)
                     /locus_tag="VF531_11395"
     CDS             complement(5415..6662)
                     /locus_tag="VF531_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7714612.1"
                     /translation="MGEIIKIAFRNIRRHKRRSILTGMAMLVGAFTLTFSQAYGTGVE
                     RQLITNMIASDTGHILITAHRQTKGSDLEEALSRRTELIPSPLPLLAELDKQPGIDSV
                     TQQLNINGMISNGIKMKQAQIIGIEPGKEQELLHRVIPAVRGRSLQKSDHYGIYISRT
                     VAATYQVTVGDVLTVIAQSVAGQSNAMDFIVKGVFDSQGSYQREQCSYILLDDAQTLI
                     QTGSGVNQIKVMLSDPGVAGDETDKLRQVFARKYNIDFTDWRTAGKFFFGVVLALQVF
                     NIILCIVLFIVAAVSVMNTTLMSVYGRIREIGTMMALGTRRYRIIGLFVTEAMLLGMI
                     AAGLGVLLGSGAALWTQYTGIPAFTESMKYAYGGNRAYPYLTIGNICLSYLVIVLLAV
                     GAALYPAIAAARMRPVEALTRNQ"
     gene            complement(6765..>7331)
                     /locus_tag="VF531_11400"
     CDS             complement(6765..>7331)
                     /locus_tag="VF531_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7714613.1"
                     /translation="AVALKPEVNQSQAIESLRREFTTAGMKLRVDGWEKSLGIFYGIL
                     TGIRVMPGMMLVVILLVVSLGIINTVLMSVLERTREIGTMLALGTRPRQVLTVFLLET
                     GILGAVAAGIGCIIGAGVIIWLGWTGIPATVDAMEFVLGGKRLFLSIDWPGLFISFGG
                     TIVLSMVASYFPARRVTRIDPVQALRQN"
BASE COUNT         1937 a         1912 c         1631 g         1851 t
ORIGIN      
        1 attacaaact tagtgccggc aatgaccagg gtattccgga ccgtctgcca aaaataaata
       61 tcggtcaaaa attggaaatt ggcaaatcca acccatttgg taccgaacgg tagatgaaac
      121 tcatccacgt tccggaaagc cattaacatt ccggccatgg gcacgtatga aaaagcaagt
      181 tgcagcaaga ccgctggtag aatcatcagt aatagccaac ggtagtcacg gacccgggcc
      241 caaaacgata cccgccgttt tctgtgggtt cgcagcattt caacttctgc gtaaccaccg
      301 gctgatcccg gttctaattc attggggtac gccatctgaa cacctccggt agatgatgtt
      361 tgtaaaaacg tttacatttt ctctgccaaa aagccttcat tgctcagcac ccgcaagatt
      421 cccggaccac aagttccggt tggataagca ggttggaata aacaacctcg gtatcaagat
      481 tgttgatgct atgaatcaat ttctcgcaac agagtacgcc gatattatag gcgggctgac
      541 tgacagttgt cagggtagga ctggtgtatt gacagatccg gatattatca aaacctatca
      601 atccaatttg ttcggggatt ttaaaaccca ttttcctggc ggcttttaat actcccaagg
      661 ccatttcgtc attaccgcaa aaaacagcat ccggcgggtc gggaagtttc ataaggcggg
      721 caaaggcgct acctgcggcc tcttcgtaat atagaccatt ttcaatatat tcgggcctga
      781 gttcccaccc gcaatctata atcgcctgtt tataaccttc caaccgttca cggcgatccg
      841 gaagatgatt ttcttcaatg ccgccgatat aggcaatctt catgaagcca tgggaataaa
      901 aatgacgtat cgcagcgtaa actccaaaac ggttatccac ggtaatggtt ggaatgttgt
      961 gttgctgcat gttccggcca aaaagcacga tgggccggcg gtcgcatttg atccggatta
     1021 actcttcatc cgtcattttg ggcatgataa agatcatccc gtcgacactg cggtcatata
     1081 aaaaacggac agaatcccgc tctttctcca gattatttcc ggcatcgcaa acaaccatcc
     1141 gcacaccgga ctcgttggcc ttgttttcga tacccttgat gacctccgca taaaatgtag
     1201 ctgttaactc cggcacaatc acgccgatga cgttcgtccg gttctttcgt agcgtggtgg
     1261 ctgaaaagtt cgtaacgtaa tttagttcct caatgacgga gattattttt ttccgggttt
     1321 cttccttgac ccgatccttc ccgttcaaca cccgggaaac ggtggctatg gaaacaccgg
     1381 ctttcttggc tacatcgtga atcgtatagt tcataacaac ctcaaatagt gtaaacgttt
     1441 ttataagtat attatattct atgaaataat tgatgtcaaa tataactgga attttgtatc
     1501 aattggatat taactcacct gctcaaagag tttttttact tgtaagactc ccctctgacc
     1561 tatcccctgc tccactcggg gcgcggatga tcaccgcaat acaaaacagc cgctgagaat
     1621 aaaagtctca acggctgctg aagatttgcc cggaaaggta atttaaatga aaaatgttcc
     1681 tttgttagct tgtattaggg acggatttta ccggcctacc tcttgaatac ggtgaagttc
     1741 ccgtaggata aaaacggcgg tcaagatcga ggccagaaaa tcggcgactg gtccggcagc
     1801 ccaaatcccg ggcaccttga aaaaacgggg caggatgagt agtgccggca ataggaaaat
     1861 cacttgccgc gacaaggtta acaaaattga ggccgtggct ttcccgatgg cctgatagta
     1921 attggagctt accacctgaa atccgatgat cggcagcatc atcaagaaaa ttcgcagccc
     1981 cgggatgccg atctcaagca gcgccttatc cgggccggcg aagatactca tgattgctcc
     2041 gggaaagagt tgcaccagga taaaacctcc ggctgtcacg gccgtggcgc ctaaaatggc
     2101 tagttccagg gtctttttta cccgggccag ttgacctgcg ccgtaattgt aaccgatgat
     2161 cggctgagcg ccttgattga tcccaatcac cggcatcaga atcaacattg agacgctgtt
     2221 gatgatgccc atggcagcga tggcgacatc tccaccgtaa gattcaaggg ataaattaaa
     2281 ggccacggtt attatgctgg ctaccaattg aaagagaaac ggggccattc cgatggctaa
     2341 aatttggccg acaaccgtcc accgtagcct taagttttcg aatttcagtt tcagtaagct
     2401 tttctttcca ataaaatatg ctacaaccca ggccgagctg accgtggaag agacgatagt
     2461 cgcaagggcc gaacccctga tccccaaatg caggacaaaa atgaagaacg ggttaaagat
     2521 ggcatttaaa accgccccaa tgagcatcgt caccatggca agccgcggat tgccctcggc
     2581 gcggataatg ttgttcagcg ccaaaccgat caactgcagt actgtccccg caatgataat
     2641 cgtgacaaac tccttggcga ggggcaacac ttcagaacta gctccaaagg cccttagaag
     2701 cggctcttca aaagccagtc ctaaaacagt tacaaagatc gatatcaaga ttgacgatat
     2761 caaagcattt cccagtattt gttcggcctc aacatgtttt ccctggccga gtttaataga
     2821 tatagtggct gccgccccga tcccgatcaa aaggccaaag gccagaatga tgatggatat
     2881 ggggaatgca accgttgttg cggagatgcc taacagcccc accccatggc ccacgaagat
     2941 ccggtccaca atattgtaaa gcgcgctcac caccataccc acaatggcgg gtaatgaaaa
     3001 attccataat aatctggcga cgcttcctgt tcctagttgt tgggcggtat ccatgcaagt
     3061 gtatctccct taattgacga tttgttcttt aaaaacccgg tttatttact ccagccaagc
     3121 cattctaaac aaaccaagta tcaaaagaca ctttcttagt ataccagatc tatccgctta
     3181 cggctattga aatccattaa ctctttttac acaagagatt tgaaagaagg aaggatgata
     3241 tttggcgcca aagggtagca atcatctccg aagaatgttg aggatcttta ttgtttatat
     3301 ttgaagaaat gattttgggg tgtcccgata aagcaacacc ccaaaacgag ttaagcggtt
     3361 ttccgcaaca tttcttttcg cacctatctc aaaaattaag aaccaatttc gcaaggatct
     3421 gatattgtga aagctccgat ccaaattcgc ttgcctcatc cccgttactg aagttcacca
     3481 tcaaaacagt aacggcttcc tgagtcaatt gatactccaa aaccggagtg agctggtaac
     3541 tgtgatcttc aatattccat aagcaattgc cggtcagact caaatcctca agtattccgg
     3601 tcttccggat caatagatat aagtagttcc ggccgagttc accaaatagg ttcgcttttt
     3661 gggcggacaa cgcggccctt tccccggggt actccgccac ataattgaag taggcttttt
     3721 cctccgggat gccccaacct tcttcattat aataatactc taattgcatc attaaattgt
     3781 ccgcaaaaat ccggttcatt cccaggactc catggagata atgcctgtca accgaacggg
     3841 tctgcaaagc aggtgtttct ccactcccgg aaacataaaa ttggcttccg gttcgtaaag
     3901 caagctcgct atagacggtg tattctccca cggttgcggc aacatcggta ccgaaggcgt
     3961 tggctgagcc ctcctggaaa agactgaaag ccagatcgca atgggggatc gcccactgca
     4021 ctttcaatgc cctaccccat tgttcccagg actccatggg ctttaaagca agggtagccg
     4081 aaaaatctcc ccggttgaac tggtaatcaa tagcatcgac ccccgggaaa ccggtagtca
     4141 gatcagcccg gtttttatcg gccccgatat aattggtcgg attccaggcc ataccgcttc
     4201 cccaggagat cttctgtctt cccacgcgta acaatgaatc ctgtgtcaat gaataatcga
     4261 tgtaggcctg attgatgaat accgactgag aacccgcagt cccaacctgt aaccaggatg
     4321 gaggattggt atagtattgc aggagtaccc gtccgcttac tttttcacta ccgttgatat
     4381 ccagcttcag attgaggttg ttgtcgaaaa atgtttcagg aggcgaagtt gccggatcac
     4441 gaaaactccg gtaggtttca ctcgactcga gacttccact ccaggcaagt tccgcgcccc
     4501 ggcagatccc cgcgggaatc aattccaacc agataacaac cccgatgaga cccagggtta
     4561 ccctttgggt taatacaaat attccggaag taaatgaaat tttcattcct ctcctcctta
     4621 cttacctttt tccaaattaa gcctggtaaa aagttgatcc gggttttcgc cggtgaaatc
     4681 agcctcaacg atatgaaaga ttgttttata ccctttccga agttcactct gaatctccat
     4741 catcgtcggg cgggttttac cgccaatcat ctgaatctga ttgtaagtca tgattttaat
     4801 taacttcccg gaagtggcat aatactcggt ccgagtcgga atgaaatcct cctgccttac
     4861 ccaaaatttc aacttgccat acgccaccga gggttgcctg gctgttaatt ccagtaagta
     4921 atgatttacc ccggcaattg actccgtccc aagcaactga gcagtataat cggtggtaag
     4981 tttgatcctc aaaatgtcgt cgttggccag atcgcttccc tgcatatttt gcctgccgct
     5041 gacccggagc gatttgttgg cgctcggcaa aaagagccat tcatcatcgc caattcgcag
     5101 ataaccctgt cccttttctt gggcgggggc cagataacgg acgagtactt tctcactgcc
     5161 tttgatatag acctgaaccc ggctggtccg ctcatccttt cccggccgga ccacagtaat
     5221 atcggctatg taccgcccgg tactggaacc gatgatctca tccacccggg cgacgatctg
     5281 atcaccgttc aattcggcga aagtctgaga tgccccggcc tgcatgattc cggcgacgat
     5341 catcccaatc aaagctactc tcaaaagatg atttttaaac attttaagac acctctcatg
     5401 ttacagccta atatttattg gtttcgcgtt aacgcttcta ccggtcgcat tcgggctgca
     5461 gcgatagccg gatacaaggc tgcacctacg gccaataaca caataaccag gtatgataag
     5521 catatattgc cgatggttag gtagggatag gcgcgattgc cgccgtaggc atacttcatt
     5581 gattctgtaa aggcaggaat acccgtatat tgggtccaga gcgccgcgcc gctcccgaga
     5641 agcactccca gtccggcagc gatcatcccc aatagcatcg cttccgtcac aaacagacca
     5701 atgatccggt accttcgggt tcccagcgcc atcatcgttc caatctcccg aatccggccg
     5761 tataccgaca tcaatgtggt attcattacg ctaaccgcgg ctacgataaa taatacaata
     5821 cataaaatga tgttaaaaac ctgcaatgcc aggacaaccc cgaagaagaa ttttcccgcg
     5881 gtccgccagt ccgtaaaatc aatattgtat ttccgggcaa aaacttggcg gagtttatcg
     5941 gtttcatccc cggcaacccc gggatcactt aacatcactt tgatttggtt aaccccggat
     6001 ccggtttgta tcagagtttg cgcatcatca agcagaatat aggaacactg ttcccgctga
     6061 taagaacctt gggaatcaaa aactcctttg acgataaagt ccattgcatt actctgaccg
     6121 gcgacggatt gggcaattac ggttagaacg tcgccaacgg taacttgata agtggcggct
     6181 accgtacggc taatgtaaat cccgtaatgg tcgctttttt gtaatgatct tccccggact
     6241 gcagggataa cccggtggag caactcctgt tccttaccgg gttcgatccc gatgatctgg
     6301 gcttgtttca tcttgattcc atttgagatc attccgttga tgttcaattg ttgagtgacg
     6361 ctgtcaatcc cgggctgttt gtccagttcc gccagtaaag gcagggggct cgggatcaat
     6421 tcagtcctac ggcttaacgc ttcttccaaa tcactaccct tggtttggcg atgggcggtg
     6481 attaaaatat ggccggtatc ggaggcgatc atattggtga tcagctgccg ctcgactccc
     6541 gtgccgtatg cctgcgagaa tgtcagtgta aaggcgccga ccagcatggc catgcctgtt
     6601 aaaatcgacc gtcgtttatg gcgccgtata ttccggaagg ctatttttat gatttcaccc
     6661 atgattctcc cccatgattc attcttcatt ccgaaaggtc ccgcggcgaa taaaccgccg
     6721 gccaggtctt ttgggtccat tcaccgcgtt aacttttgaa tgtttcaatt ctgacgcagc
     6781 gcttgaaccg gatcaatccg ggtgacccgc cgcgccggaa aataggatgc aaccatactt
     6841 aaaacgattg ttcctccaaa cgaaatgaat aaacccggcc aatcaatgct gaggaaaagg
     6901 cgttttccac ccagcacaaa ctccattgca tccactgtgg ccggaatacc ggtccatccc
     6961 agccaaatga tgacccctgc cccgatgata cagccgattc cggcggcaac cgcgcccaag
     7021 attcccgttt ccaggagaaa gactgttaaa acctgtcggg gccgggttcc cagggccagc
     7081 atggttccga tctcccgggt cctctccagt accgacatga gtaccgtatt gataataccg
     7141 agcgaaacca caagtaaaat tacaacgagc atcattcccg gcatcacccg gataccggtg
     7201 agaattccgt aaaagattcc cagcgatttc tcccagccgt ccacccggag tttcatcccg
     7261 gcggttgtga attcccgtcg taaactttca atcgcctggg attgatttac ctccggctta
     7321 agtgccaccg c
//
LOCUS       DASVMB010000403         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_274661,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000403 DASVMB010000000
VERSION     DASVMB010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274661"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /gene="yfcE"
                     /locus_tag="VF531_11405"
     CDS             complement(<1..237)
                     /gene="yfcE"
                     /locus_tag="VF531_11405"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX7714614.1"
                     /translation="MLIGIISDTHGSLTAWRLAYEKFFRDVDLIVHCGDVLYHGPRNP
                     LPEGYDPKSLAAELNSLQKPMIVVRGNCDAEVDQM"
     gene            complement(300..710)
                     /locus_tag="VF531_11410"
     CDS             complement(300..710)
                     /locus_tag="VF531_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7714615.1"
                     /translation="MDYSIKQVSEKTNLKAYVLRYYEKEGLLPLVNRTRSGIRRYSEE
                     DLEWLSLICCLKNTGMSIKQIKDFVVLSQQGGETLKQRCEMLIEHKKNVEAQIAEMQR
                     HLQKVDHKIERFTEQYERYMAEHPDDSAVIPKVC"
     gene            818..1669
                     /locus_tag="VF531_11415"
     CDS             818..1669
                     /locus_tag="VF531_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7714616.1"
                     /translation="MKSLKDCYHLHNGVQIPCIGFGTWKAPDGEIAVSAVKEAIRLGY
                     RHVDTAAAYGNEASVGSAVKQSGIPREEIFITSKLQNVAHGYEETMAAFAQTMKNLDL
                     EYLDLYLIHWPNPVKYRSCWQEANAGTWKAFEELYQSGRIRAIGVSNFRPHHLDELMK
                     TATITPMVNQIRLCPGETQDEVVAYCKARNILLEAYSPLGAGSIFEVPEMQALAEKYG
                     KSIAQICIRWSLQMGFLPLPKSVTAARIKENAEVFDFELAAEDVRMIADLKGCCGYSQ
                     NPDMTSF"
     gene            complement(2618..3556)
                     /locus_tag="VF531_11420"
     CDS             complement(2618..3556)
                     /locus_tag="VF531_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEX7714617.1"
                     /translation="MIVNSIYMPLIQTVRGFNRFYTNILGLLDRHLLDSQFSLSEVRV
                     LYEIGHTENCTAKNLSEELRIDPGYLSRMLKYFEKRMLTYRVKSAKDARLYYLYLTDQ
                     GKETLAKLEELSNRQIDQMIGRLPEPDQRKLVEGMQTIENVLSEKPGPVREKVIIRNE
                     LKPGDAGDLIHLHGRIYAAECGYNHVFEGYVCKTLYDFLVNYSPEKDRIWFAEAGGRM
                     IGAIAVFGHSPQKAQLRWFILHPQFRGIGLGGTLFDKALQYCREKGFQKVFLETTTDQ
                     KTAIKMYTKAGFQKVAEHENHAWGKNLVELTFELDL"
BASE COUNT          906 a          798 c          851 g         1014 t
ORIGIN      
        1 catttgatcg acctcggcgt cgcaattgcc gcgtacgaca atcatcggtt tttggagtga
       61 gttcagttca gcggcgaggc ttttcggatc atacccctcc ggcaacgggt tgcgcgggcc
      121 gtgatagaga acatccccgc aatgaacgat caggtcgaca tcgcgaaaga atttttcgta
      181 agccaaccgc caggcggtga gtgaaccatg agtgtcgctg atgattccga tgagcatatt
      241 gttctccttt gaaaccaaga tgttttcttc tttataggaa tagggccaac tatccttact
      301 tagcagactt tgggtatgac tgcggaatca tccgggtgct ccgccatata tctttcatat
      361 tgctccgtga agcgctcaat cttgtgatcc accttttgca aatgcctttg catctccgcg
      421 atttgggctt ccacgttttt cttgtgctcg atcagcatct cgcaacgttg tttgagagtt
      481 tccccgcctt gttgactgag tacaacaaaa tctttgattt gcttaatcga cattccggtg
      541 tttttgaggc agcaaatcag gcttagccat tccaaatcct cctccgaata acggcggatc
      601 ccgcttctag tccggttcac cagcggcagc aagccttcct tttcataata acgcaacaca
      661 tatgctttca ggttggtttt ttccgatacc tgtttaatcg agtaatccat acaatttccc
      721 ttcctcgttt tatttttagg attgacttaa agttaacgtt aagatttaca attatactat
      781 acttcatctt aggccatatc ctcaaggagg aattctgatg aaatcattga aggattgcta
      841 ccatttacac aatggggttc aaattccttg tatcggcttt ggtacttgga aggcgcccga
      901 tggagagatt gcggtgtctg ccgtgaagga agccattaga cttgggtatc gacatgttga
      961 taccgcggca gcctatggca atgaggcaag cgtcggcagc gccgtcaagc aaagcggcat
     1021 tcccagagaa gagattttca tcaccagcaa gctgcaaaat gtagcccatg gctatgagga
     1081 gacaatggct gcctttgcgc agaccatgaa gaatctcgat ctggaatatc tggatctgta
     1141 tctgattcat tggccgaatc ccgtcaagta cagaagttgc tggcaggaag ccaacgccgg
     1201 cacctggaag gcgtttgagg aattgtatca aagcggccgt atccgggcca tcggggtcag
     1261 caatttccgt ccgcatcatc tcgatgaact tatgaaaacc gcgacgatta caccgatggt
     1321 caatcagatc cggctttgcc ccggggaaac ccaggatgag gtggtggcct actgcaaggc
     1381 ccgtaacatc ctgcttgaag cgtacagtcc gttgggagca ggttcgatct ttgaagtccc
     1441 ggaaatgcag gccctggcgg aaaagtacgg caagagcatc gctcagatct gcatccgctg
     1501 gagcctgcaa atgggctttt tgcccctgcc caaatcggtg acggcagccc gcatcaagga
     1561 aaacgccgag gttttcgatt ttgagcttgc ggcggaggat gtgcggatga ttgccgatct
     1621 taaaggctgc tgtggatatt cccaaaatcc cgacatgacc tcgttttaaa aagtttcaag
     1681 cgaagcctac ggcaggaaca tccgattggg ggactgaaga agtaatactg gcaggatgtg
     1741 ctggagattt aagtaaggtg tttgggttgt tgctccggcg attgacagat cagttaaaaa
     1801 tgctctcttt tcatctgaag aagcctcatc aaagaggctt gaacggtgga aggggagttg
     1861 tttttcgtgg ggaatagggg gattatatca aaaccatgta tatgagcagt taatggccaa
     1921 ccatcttggt tttaaatacg aaggcattct gcttggaagc ccccatagtt ggagtattaa
     1981 tggaaatact attcaggaca ggctggttag ggatagatat tccgaacatg aatcgcaatg
     2041 agagaggaag gcgtttatga gtaattggaa agatgatgat aaaactctta tttgggtact
     2101 aagtggtatc atttcgaccg tcatcttttc ggacgcatat tcgatattgc tgaaattagt
     2161 gggtgccgaa ccacagtatt cgtggaattt tgccgctgat tatatcatcc aagggcgccg
     2221 gaatattgaa agcttttatg gaataatcat cggaataacc gttaattttt tcatcggaag
     2281 cataaatgga tttttgattg gccttatttt ggattggcgg ggacccaaat attattggat
     2341 gaaagggatt ggaataggct tgtccaattg gatcgcgttg gggattatga cccaagtatg
     2401 tccccaatta tttacttatc atttatcgcc gttgaattat ttcacttata tatttagtta
     2461 cttggcattt ggcgctttta ctgcatactt aattgtcaaa tggtccaagt gaactgtggc
     2521 tgccgtcggt gaggacaagt tggaactgat gacaaaaaag caaccggtga agcgtacggc
     2581 agagagcaaa gttacttgat taagttaaat taaattatca taaatccaac tcaaaggtta
     2641 gctcaaccaa attttttccc caggcatggt tttcatgctc ggctactttt tgaaatccgg
     2701 ctttggtata catcttaatc gccgtttttt ggtcggtggt ggtctccaaa aatactttct
     2761 gaaatccttt ttccctgcaa tattggaggg ctttatcgaa cagtgtgccg cccaggccga
     2821 tgcctctgaa ttgtggatgc aggatgaacc atcgtaactg agccttttgc ggcgaatgtc
     2881 cgaaaacggc aattgcgcca atcatcctgc caccagcttc ggcgaaccaa atccggtcct
     2941 tctcaggact gtagtttacc agaaaatcat ataaagtctt acagacatag ccttcaaata
     3001 catgattgta tccgcattcc gccgcataga tccggccgtg gaggtgaatt aaatctccgg
     3061 catcccccgg tttcagttca ttacggataa tcaccttttc cctgacaggc cccggttttt
     3121 cagacagcac attctcaatt gtctgcatcc cttcgaccaa ttttctttga tctggctcag
     3181 gcagccggcc tatcatttga tcgatttgcc ggttggacag ttcctccagc ttcgcgaggg
     3241 tttccttgcc ttgatccgtt aaataaagat aatataaccg ggcgtctttc gcggacttca
     3301 ccctgtatgt aagcatacgt ttttcaaagt attttagcat tctgcttaaa taccccggat
     3361 cgattcttag ttcttcactt agatttttgg cggtgcaatt ctccgtatgg ccaatttcat
     3421 acaatacacg gacttccgat agagaaaatt ggctgtctag cagatgccga tccaaaagcc
     3481 cgaggatgtt ggtatagaac cggttaaacc cgcggacggt ctggatcaaa ggcatataga
     3541 tggaattcac gatcatccct ccatgaatt
//
LOCUS       DASVMB010000404         9004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_346671,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000404 DASVMB010000000
VERSION     DASVMB010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346671"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF531_11425"
     CDS             <1..591
                     /locus_tag="VF531_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX7714618.1"
                     /translation="FDDQLGNHDVKGFRGHFRVLFREKRLTTGGFKAIFGAVIALVFS
                     LGTLELTKNQWPIWTLISNFLLVALSANSINLSDLRPGRAGKFYLFGFILIVIFSRNS
                     ESHLGLFLPVLATLLYYLPYDLRAEVMMGDSGSNLLGASLGMMMAWMFSDMSKAIALI
                     LLLGLQLLAEKYSISAIIERTSWLKYLDELGRRRKA"
     gene            591..1424
                     /locus_tag="VF531_11430"
     CDS             591..1424
                     /locus_tag="VF531_11430"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX7714619.1"
                     /translation="MRFGVHVSIGKGFEQAVKTAVTLGCDGFQIFAGNPRGWARKPMV
                     SQDIEAFHTARQHSKVYPVMVHLSYLPNLAAEDPLLFDKSLKAFREDYQRASLLGADF
                     FVFHPGKSKSRDRGLTQIIAVVKQTLEEVSGSTILLFENQAGAGAEIAADFQDLGRLI
                     QGVDAKKRIGICLDTCHAFAAGYDLRNRRGWETVLSDFDSRVGLEYLKIFHLNDAMGD
                     LGSHLDRHQHIGAGEIGLEGFAFLVNHPQLSQLPGILETPQDNPEDDRKNLATLRGLI
                     S"
     gene            1445..1900
                     /gene="rnhA"
                     /locus_tag="VF531_11435"
     CDS             1445..1900
                     /gene="rnhA"
                     /locus_tag="VF531_11435"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX7714620.1"
                     /translation="MKLVEIYTDGACSGNPGPGGWAAILICAGVEKELSGSVSATTNQ
                     RMELTAAVEALQALKYPCRIRLYSDSAYLINAFRQNWIAGWQANGWRNAQKKPVENQD
                     LWRSLIALREVHRIEWLKVAGHQDNQYNNRCDELARKAITLLKGTVETK"
     gene            1941..2759
                     /locus_tag="VF531_11440"
     CDS             1941..2759
                     /locus_tag="VF531_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7714621.1"
                     /translation="MEEKEQNDLTERARRIRIAIIQMIAAAQSGHPGGSLSAVELLTY
                     LYFHKMRLDPENPQWAERDRFILSKGHAAPALYAALAERGYFDKAILLTLRRFQSILQ
                     GHPDMRKTPGVEISGGSLGQGLSVGNGMALAARLNHLDYRVYVLLGDGELEEGQIWEA
                     LMTSVHYKLDHLTAFLDYNRLQIDGLVEEVKSLNDPVRRFAAFGWRVFEVDGHDFTAI
                     DRAVTEAEATLGQPSMIVMHTCKGKGCSFMENQVGWHGKALQAEQVERALQELA"
     gene            2917..3861
                     /locus_tag="VF531_11445"
     CDS             2917..3861
                     /locus_tag="VF531_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX7714622.1"
                     /translation="MSEKVATRDAYGNALVKLGEINANLVVLDADLSKSTKTAIFAGK
                     FPERFFQMGIAEADMISTAAGLAAAGKIPFASTFAIFATGRAYDQLRNTVAYSRLNVK
                     IAATHAGLTVGEDGGSHQALEDITLMRAIPGMTVLNPADGVETEKLVFAAADYPGPVY
                     IRLGREPVSIIFSADYQPEIGKAYRIREGKAATIVATGIMAEQAIHAAEILSNRGSTV
                     RVLDMHTIKPLDAAEIIKAAWETGAIVTAEEHTVIGGLASAVAEVVVQNHPVPMEFIG
                     TRDLFGQSGTPAELLAAYGLGTDHIVAAVEKAIERKRS"
     gene            4132..4740
                     /locus_tag="VF531_11450"
     CDS             4132..4740
                     /locus_tag="VF531_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714623.1"
                     /translation="MDRKSWLFACCALWVALVIWPDQAYAANQPYTVNSPGSPDFTAD
                     ITLTDINGKVVTGKIFVKGGSVRQELLHPEGILITILRKDKSVFWTLMPGKRYMEMKN
                     PVDPTEPQRVEFERELSGIESINGYDCEIIRYKYKNRTYGMFTVWFASKLNYGIRTET
                     QSASGTIIATSECRNIKESTLNDALFEIPPGYVKYSLFKMRS"
     gene            4947..5900
                     /locus_tag="VF531_11455"
     CDS             4947..5900
                     /locus_tag="VF531_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714624.1"
                     /translation="MKTGKELRKLFPGSVAFSFLLLVLGLIMVLIPLTPGIGGNIALF
                     LTGLGSAMFLAGFLGFIYVAILAEEFRRMSDQPFNQMVFLSKIQNSGLLDIFRNRLEG
                     TAEMIRSLEDENKQFTIVGSSLRGLVGVAFDEETEYSEIWKAIKDALQRKVKVNILLT
                     HPEAAHHRAIAEGRLEGDIETDILENLIYLVMYKMENPKLAEYLSVKLYSGTPTIFMI
                     CTSQKMLLNPYPYYATAASSYAFLVDGGSPLYTYYYHSHYRAAWLDSRSTIDIKEDPK
                     EAVKQIQELINMNSRDKNGLIVPDETKRNAMLQTLYTMLQN"
     gene            5963..6556
                     /locus_tag="VF531_11460"
     CDS             5963..6556
                     /locus_tag="VF531_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714625.1"
                     /translation="MGKIKTMLLGLLCVAMVLGITGCVPSLQPLYTDEDLVTDSRLDG
                     LWLEGEDLTTGKAKSTWNFQKITDKQAYEAILTQDGKTSRFSVHLVKLGGNLFMDTYP
                     MEMNLESANEYYKMHFIKGHIIARINFGEKNVLRVATLNYNWLARQYKDGKLTLPHEE
                     VENQIILTGSIHELQQFLKEHGDDAFGEPIVLRRKRG"
     gene            6741..>9004
                     /gene="yicI"
                     /locus_tag="VF531_11465"
     CDS             6741..>9004
                     /gene="yicI"
                     /locus_tag="VF531_11465"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEX7714626.1"
                     /translation="MKFSDGFWRNREGVNVYSPAEIYDYEIDQHSITVYTPYFHIANR
                     GQTLTGPLLTIRISSPLEGIIGVQVSHFTGVVPKTPSFALHRNPDLKVEVGETENDIF
                     LKSGNLSVRLNKTSWIMEFYDGDRKITDTGNKNLAYIKTSDNQCYMREQLALSVGESV
                     YGLGERFTPFVKNGQVVDIWNADGGTSSELAYKNIPFYITNKGYGVFVNDPGRASFEV
                     GSEKVSRVQFSVPGEYLEYYVIGGPTPKETLTRYTDLTGRPALPPAWSFGLWLTTSFI
                     TNYDEKTVTAFIDGMAERDLPLHVFHFDCFWMREYNWCDLEWDQRQFPEPGPMLERLK
                     ARGLKICVWINPYIAQRSQLFAEGKANGYLIKKPNGDVWQCDDWQPGMGIVDFTNPEA
                     CRWYAAKLRELMDMGVDCFKTDFGERIPTDGVYSDGSDPEKMHNYYTFLYNRTVFEAL
                     QEKFGAGNSLVFARSATAGGQQFPVHWGGDCTANYESMAETLRGGLSLGISGFGFWSH
                     DISGFEHTATPDLYKRWTAFGLLSSHSRLHGNMSYRVPWLFDEESVDVLRFFTKLKCR
                     LMPYLFAAACEAARTGAPVMRAMVLEFPGDPFCDHLDRQYMLGESLLVAPIFNDRGTA
                     SYYLPQGRWTNFLTGETVEGGSMRNERHGYLSIPLWVREGSLIAVGSVDTRPDYDYAT
                     GIELQLFEVQDGGTASATVYNTAAEPELKVTVCREGAKIVITSSGTGKPWSLLLRGAH
                     TVKTVSGAAHEADTL"
BASE COUNT         2292 a         2001 c         2361 g         2350 t
ORIGIN      
        1 ttcgatgatc aactaggaaa tcatgatgtc aaaggattcc ggggccattt ccgtgtgcta
       61 tttcgcgaga aacgcctgac aaccggaggg tttaaggcca tcttcggtgc ggtgatcgca
      121 ctcgttttca gtctgggaac cttggagttg acaaagaacc aatggccgat ttggacctta
      181 atcagtaatt ttctactcgt tgctttgtcc gccaattcca ttaatctttc cgatctgcgt
      241 ccgggacgcg ccggaaagtt ttatctcttc ggttttattc ttattgtaat tttcagccgc
      301 aactccgagt cgcaccttgg tttattcctg cccgttctgg cgactctttt atattacctg
      361 ccttatgatc tccgggccga ggtgatgatg ggtgacagcg gttcgaatct cctgggagcc
      421 tctttgggaa tgatgatggc ctggatgttc agtgatatga gtaaggccat cgccttgatt
      481 ttgcttttgg gtttacaact tttggcggaa aaatactcaa ttagcgctat tatcgagcgg
      541 acttcctggt taaaatatct tgatgaattg ggtcgtcgga ggaaggctta atgcgcttcg
      601 gagtccatgt ctccatcgga aagggatttg aacaagcagt gaaaactgcg gtcactttgg
      661 gttgcgatgg ttttcagatc tttgccggca atccccgggg ttgggcgcgg aagcccatgg
      721 tttcccagga tattgaggcc tttcataccg cccgccaaca ttccaaagtt tatccggtaa
      781 tggttcatct ttcatattta ccgaatctgg cggcggagga tccgctcctg tttgataaat
      841 ctttaaaagc ttttcgcgag gattatcagc gcgccagcct cctgggagcg gatttttttg
      901 tctttcaccc aggtaaatcc aagtctcgcg accggggatt gacacagatt attgcggtgg
      961 tcaaacaaac tttggaagag gtttccggat ctactatatt gctctttgaa aatcaagccg
     1021 gagcgggggc ggaaattgcc gcggattttc aggatttggg ccggctgatc cagggcgtgg
     1081 atgccaagaa acggatcggg atttgtttgg atacatgcca tgcctttgcc gctgggtatg
     1141 accttcggaa ccggcgcggt tgggaaactg tgctttctga ttttgattcc cgggtgggtt
     1201 tggaatatct aaaaatattc catcttaacg acgccatggg cgatttggga tcccatctcg
     1261 acaggcatca gcatatcggc gcgggtgaga ttggactgga aggattcgcg tttctcgtta
     1321 accatccgca actctctcaa cttccgggaa tcttggaaac accccaggat aatccggagg
     1381 atgaccgaaa aaatttagcc acgctgaggg gcctaatctc atgattaaat ctgatcctgc
     1441 cgagatgaaa ctggttgaga tctatacaga tggcgcatgt tccgggaatc cgggacccgg
     1501 cggctgggct gctattttga tctgcgccgg agtagaaaag gaactctccg gatcagtgtc
     1561 tgcaactaca aaccagcgta tggaacttac cgctgcggtc gaagcattac aggccttaaa
     1621 atatccttgc cggatccgcc tctattcaga cagcgcctat ctgattaatg cttttcgcca
     1681 gaactggatt gcaggttggc aggctaacgg ctggcggaat gcacagaaaa aacccgtgga
     1741 aaatcaggat ttatggcgaa gcctgatagc gctccgagag gtgcaccgga tagaatggtt
     1801 aaaagtcgcc gggcatcagg ataaccagta caataaccgt tgtgacgaat tggcacggaa
     1861 ggctataacc cttttgaaag gtacggttga aacaaagtga agcggttttt taagactcaa
     1921 tgaaggagtg aaatggtttg ctggaagaga aagaacaaaa cgatttaacg gaacgagctc
     1981 gccggatccg gattgcaatc attcagatga tagccgcagc ccaatcaggg catcccggcg
     2041 gttccctttc agctgtcgaa ctacttactt atctctattt tcataaaatg cggctcgatc
     2101 ccgaaaaccc gcaatgggcg gaacgggatc gttttattct gagtaagggg catgccgctc
     2161 cggcacttta tgccgctcta gcggagcggg gttatttcga caaagccatt ctattgaccc
     2221 tgcggcgttt tcaaagtatc ctgcaggggc accccgatat gcggaaaact cccggagtgg
     2281 agatctccgg cggctctctg ggacagggat tatcggtggg taatggaatg gctttggcgg
     2341 cccggttgaa ccatttggat tatcgggtat atgttttatt gggcgatggt gaactcgagg
     2401 aaggacaaat ctgggaggca ttgatgacct cggttcatta taagctggat catctcaccg
     2461 cttttcttga ttataaccgg ctacagatag acggactcgt ggaagaggtg aaatccctca
     2521 acgatccggt aaggcgtttt gcagctttcg gctggcgcgt atttgaagta gacggtcatg
     2581 attttactgc catcgatcgg gcggtaaccg aggctgaagc aacgcttggc cagccgagca
     2641 tgatagtgat gcatacctgt aaaggtaaag gctgctcttt catggaaaat caggtgggat
     2701 ggcacggtaa agcgctccag gcggaacagg ttgagcgggc attacaggag ttggcataaa
     2761 atcaggcaag aggcaacagg caacagtgat taaagattta cggtaagata aaaatcactt
     2821 gcaaaacttg tccttattgc ctgttctatt gcctggtccc tgttgcctat tgcctttaaa
     2881 atgtagacac aaattttaaa atgagagggg taacccatga gcgaaaaagt tgccacccgt
     2941 gatgcttacg gtaatgccct ggttaaactg ggtgaaatta acgctaatct ggtggtgttg
     3001 gatgccgatc tgtccaaatc caccaaaact gcaattttcg ccggtaaatt tccggaacgt
     3061 ttttttcaga tgggaatcgc tgaagccgat atgatttcca ctgccgccgg gttggccgcg
     3121 gcgggcaaga ttcccttcgc cagtactttc gcgatcttcg ccaccggccg ggcttatgat
     3181 cagctccgga acaccgtggc ttattcacgg ctaaacgtaa agattgcggc gacccatgca
     3241 ggactgaccg tcggtgagga cggcggatcc catcaagccc ttgaggacat tacactgatg
     3301 cgcgccatcc ccggaatgac cgttttaaat ccagccgatg gagtagaaac agagaaactg
     3361 gttttcgctg ctgccgacta tcccggaccg gtttatatcc gccttgggcg ggaaccggtc
     3421 tccatcatct tttccgctga ttatcaaccg gagatcggaa aagcttaccg aatccgtgaa
     3481 gggaaggcgg ccaccattgt cgcaaccggt atcatggctg aacaggcaat tcacgcggcg
     3541 gagatcttat cgaatcgtgg gagtaccgta agagttcttg atatgcatac tataaaaccc
     3601 cttgacgcgg cggaaatcat taaggcagcc tgggaaaccg gcgcgattgt tactgccgag
     3661 gaacacactg tcatcggggg actggccagc gctgtcgccg aagtggtggt tcaaaaccat
     3721 ccggttccaa tggagttcat cggtacccga gatcttttcg gccaatcggg aacccctgcc
     3781 gagttattgg ctgcatacgg actaggcacc gatcatattg tagccgcagt cgagaaggcc
     3841 attgaacgga aaaggtccta accaagttaa cccttacttg acaaaacccg gcagatggag
     3901 tatatataaa aaatgaacat ggcttttcat gcggtttgac aaggcaagcg aaacgacctg
     3961 gtcaccgaga tttgcacatc ttaaagaggg aatgtaaaaa cccgtggggc attcatgttt
     4021 cacgggttat tatattgaaa ctttgcctat ggtctatctg agagcggata gcgtattgta
     4081 tccgttaatc tatcgtcggt tttcggtgta tgttggaagg gggcgttggt aatggaccgg
     4141 aaatcttggc tatttgcgtg ttgtgctcta tgggttgctt tggtgatttg gccggatcag
     4201 gcttatgctg ccaatcagcc atatactgtg aattcaccgg gttcccctga ttttacggca
     4261 gacattactt tgacggatat taacggcaag gtagtaacgg ggaaaatttt cgtcaagggg
     4321 gggagtgtcc ggcaggaatt gctgcatccc gagggaattt taattaccat tttacgcaag
     4381 gataaatcgg tcttttggac cttaatgccc gggaaacgat acatggagat gaaaaatccg
     4441 gttgatccca ccgagccgca acgggtggag tttgaacggg aattatcggg tattgaatcg
     4501 ataaatggtt atgattgtga gatcattcgg tataaatata aaaatcgcac ttatggtatg
     4561 tttacggttt ggttcgcatc gaaattgaat tatggaattc ggaccgagac ccaaagcgct
     4621 agtggtacca tcatagctac cagtgaatgc aggaatatca aggaaagcac attaaatgac
     4681 gcgctgtttg agattccgcc ggggtatgtc aagtattcgc tctttaagat gagatcctga
     4741 gttaaaaaca gccctttcat acaggctgtt ttttcattat ttatgaactc cccaaaatga
     4801 aagggatttt tttatcgaag gaaaccttga agaaacatcc aaggttttta ttcccggctg
     4861 cggcaggatt ttgacgggac aggctagaat atgatgaaaa gagttttgac ttacattttt
     4921 ttcataatag gaggttccga ttacgcatga aaaccgggaa ggaactccgg aagttgttcc
     4981 ctggttctgt ggccttcagt tttttattgc tggttttggg tttgataatg gtacttatcc
     5041 cactgacgcc cggtattgga gggaacatcg ccttgttcct caccggactg ggttccgcca
     5101 tgttcttggc tggtttttta ggatttattt atgttgcaat cttagcggaa gagtttcgac
     5161 ggatgagtga ccaacctttc aaccagatgg ttttcttatc caagattcaa aatagcggcc
     5221 tcttagatat tttccgcaac cggctggaag gtacggcgga gatgattcga agtctggaag
     5281 atgagaacaa acagtttacc atcgtgggtt cgtccttacg aggccttgta ggagtggcct
     5341 tcgatgaaga aacagaatat tccgaaatat ggaaggcaat aaaggatgcc ttgcaacgta
     5401 aggtaaaagt caatatcctg ctgactcatc cggaggcggc ccatcaccgc gccatcgccg
     5461 agggtcgtct tgagggcgac atcgagaccg atatcctgga gaacctgatt tatctcgtga
     5521 tgtacaagat ggaaaatcca aagctcgcgg agtatctaag tgtcaagtta tatagcggta
     5581 ccccgacgat ctttatgatc tgtacctctc aaaaaatgtt gctcaatcca tatccctatt
     5641 acgcaactgc ggcgagttcg tatgcgtttt tggtagatgg cggctcccct ttatacactt
     5701 attattatca ttctcactac cgcgcggctt ggcttgactc aaggagtact atcgatatta
     5761 aagaagatcc caaggaagcg gtcaaacaga ttcaggaatt aatcaatatg aatagccgtg
     5821 acaagaacgg tttaatcgtc ccggatgaaa cgaaacgaaa cgcaatgttg cagacattat
     5881 acaccatgtt gcaaaattag gtaccggaag ggtcacgcca tggcgtgatc tagccatagg
     5941 gtatggaaga gaggttaata aaatgggtaa aatcaaaacc atgcttttag gtttgctttg
     6001 tgtggcgatg gttttgggga ttacgggttg cgttccatca ctgcagccct tgtataccga
     6061 tgaagatttg gtcaccgact cccggcttga cgggctttgg cttgagggag aggacctgac
     6121 gacgggtaag gctaaaagca cctggaactt tcaaaagata acagacaaac aagcttatga
     6181 ggcaatcctg acccaggacg ggaaaacgag ccggtttagc gtacatcttg tcaaactggg
     6241 cggcaatttg tttatggata cttatcccat ggagatgaat cttgaaagcg ccaatgaata
     6301 ttataaaatg cattttataa agggacatat catcgccagg atcaacttcg gtgaaaaaaa
     6361 tgtactgcgg gtggcaacct taaattacaa ttggttagcc cgccagtaca aagatggaaa
     6421 actgacttta ccccatgagg aagtagagaa tcagatcatc ctcaccggat ccatccatga
     6481 gttgcagcaa tttctaaaag aacatggcga tgacgccttc ggagaaccta tcgtacttcg
     6541 ccgcaaacgg ggttaggtca tataggcaac aggcaatagg gaacaggcaa taggcaataa
     6601 acaggggctg tccgaattta gggatcagcc cttatttttg ttgaatgatt agtttgaagg
     6661 aagcaggaga atagttggag aagaccgaat aaaacaaatc tgtaaaaaat gaatgaattt
     6721 taggaaagag tgatcgaaac atgaaattta gtgacggttt ttggcgcaac cgggaagggg
     6781 ttaatgtata tagcccggcc gagatctacg attatgagat cgatcagcat tcgataactg
     6841 tttatactcc atacttccac attgctaacc ggggacaaac ccttacagga ccactattga
     6901 ccatccggat ctcatcgccg ctcgaaggaa tcatcggtgt gcaagtatct catttcacgg
     6961 gagttgttcc caaaactcct tcttttgccc tccatcgcaa tcccgacctc aaggtcgagg
     7021 tgggtgaaac cgagaatgat attttcttaa aaagcggcaa tttaagtgtc cggttgaaca
     7081 aaacctcttg gatcatggag ttttacgacg gtgacagaaa aatcaccgat accggtaata
     7141 agaacctggc ctatattaaa acttccgaca accaatgcta catgcgagaa caattggccc
     7201 tttccgtcgg agaatccgta tacggactcg gggaacgatt cacccccttc gtaaaaaacg
     7261 gtcaggtggt tgatatttgg aatgcagacg gcgggaccag ttcagagctg gcttataaga
     7321 acattccctt ttatatcacc aacaaagggt atggcgtttt cgtcaacgat cccggccgcg
     7381 cctctttcga agtgggttcg gagaaggttt cgcgcgtcca gttcagtgta ccgggtgaat
     7441 atctggagta ctacgtcatt ggaggcccca cccccaagga aacccttacc cggtatacgg
     7501 atcttacagg acgtccggct ttaccgccgg cctggtcgtt cggtttgtgg ctaaccacct
     7561 ctttcatcac caattatgat gaaaaaaccg tcaccgcttt cattgacggc atggccgagc
     7621 gggatttgcc gcttcatgta tttcatttcg attgtttctg gatgcgggag tataactggt
     7681 gtgatcttga atgggatcaa cggcagtttc ccgagcccgg accgatgcta gagcggttga
     7741 aagcgagggg attgaagatc tgcgtttgga tcaaccctta tattgcccaa cggtctcaac
     7801 tttttgcaga gggaaaagcc aatggttacc tgatcaaaaa gcctaacggc gatgtctggc
     7861 agtgcgacga ttggcagccg ggaatgggta ttgtcgactt taccaatccg gaggcctgca
     7921 ggtggtatgc ggcaaaatta cgcgaattga tggatatggg tgtggattgt ttcaagaccg
     7981 atttcgggga acgtatcccc accgatgggg tttactccga cggttccgat ccggaaaaaa
     8041 tgcataatta ctatactttt ttgtataacc gtacagtttt cgaagcgctc caagaaaagt
     8101 tcggcgccgg caattcattg gttttcgccc gatcggccac tgccggaggg caacaattcc
     8161 cggttcattg gggtggagat tgcacggcca attatgaatc gatggccgaa accctgagag
     8221 gtggattatc tttgggtatt tccggtttcg gtttctggtc ccatgacatc agcggtttcg
     8281 agcatacagc cactccggat ctttataaac gctggactgc ttttgggctt ttatcatccc
     8341 acagccggct ccatgggaac atgtcctatc gtgtgccttg gctattcgac gaagaatccg
     8401 ttgatgtact acgttttttc accaagttga aatgccggtt gatgccgtat ctgtttgccg
     8461 cagcttgtga agcagcccga accggagcac cggtaatgcg cgccatggtt ttggagttcc
     8521 cgggcgatcc attctgtgac cacttggatc gccagtacat gttgggggaa tcgttgctgg
     8581 tagcgccgat cttcaatgac cggggaaccg cctcctatta tctgccgcag ggccgttgga
     8641 ccaatttcct taccggcgaa acggtagagg gtggctcgat gcgaaatgag cgccatggtt
     8701 atctgagtat tccgctttgg gtccgggagg gcagtttgat cgctgtcggt tcggtagata
     8761 ccaggcctga ttatgactac gccaccggga tcgaattgca gctttttgaa gttcaggatg
     8821 gaggtacggc ttccgccaca gtttataaca ctgccgcaga gccggagttg aaggtcactg
     8881 tttgtcgtga gggagcaaaa attgtgatta cttcctccgg tacaggtaaa ccttggagcc
     8941 tgctactccg tggtgcacat accgtaaaaa ccgtttcggg agccgcccat gaagcggata
     9001 ctct
//
LOCUS       DASVMB010000405         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_419675,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000405 DASVMB010000000
VERSION     DASVMB010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419675"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF531_11470"
     CDS             <1..403
                     /locus_tag="VF531_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714627.1"
                     /translation="EQDALLLIQRMMGNKRLKSINRVEVSVLTELVNILANSFWRALT
                     EQAQLKWWITPPSRVNELSRALTYSAKVYTLDHLLIHLEYLIPMMEIRIQFTMLPTKS
                     TIKKILTKLNPIQYIHEELEPELPLKVEPGA"
     gene            505..975
                     /locus_tag="VF531_11475"
     CDS             505..975
                     /locus_tag="VF531_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX7714628.1"
                     /translation="MRWRRPRISIGLFIIGMVLIGSAVIFKYTSAEPAAPNRQSGDVE
                     LLARVIRAEAEAEPFTGQVAVGAVIMNRIQNSKFPKTIAGVIYQPSAFESVSNGEINR
                     AATDSARRAAQSAISGWDPSGGALFFFNPAKVGARSYVWTRKIIQQIGKHVFAL"
     gene            1092..1538
                     /locus_tag="VF531_11480"
     CDS             1092..1538
                     /locus_tag="VF531_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7714629.1"
                     /translation="MLLRERLPEDLKTAMKNRETLRLSVIRMAKAAVMNQEIARGHEL
                     SDDEVIEVLAKEVKQRSDSIPEYERGNRPDIIDQLKQEIAILKGYLPAQLSEDELRRI
                     IQETVSAIGATSKKEMGKVMAALMPKVRGRADGKLVNEIVGSLLQG"
     gene            1602..1787
                     /locus_tag="VF531_11485"
     CDS             1602..1787
                     /locus_tag="VF531_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714630.1"
                     /translation="MTERTVKKELHVRVDPELYEKLRQITFYERQTFSGVVNRLLIEY
                     VEEKAIEEPEIVAPPVL"
     gene            complement(1867..2037)
                     /locus_tag="VF531_11490"
     CDS             complement(1867..2037)
                     /locus_tag="VF531_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7714631.1"
                     /translation="MSYKISDECISCGACQSECPVEAISEGSDKYVIDPGKCIECGAC
                     ADVCPVGAPKQG"
     gene            2240..2314
                     /locus_tag="VF531_11495"
     tRNA            2240..2314
                     /locus_tag="VF531_11495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2272..2274,aa:Gly,seq:ccc)
     gene            2471..2545
                     /locus_tag="VF531_11500"
     tRNA            2471..2545
                     /locus_tag="VF531_11500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2503..2505,aa:Thr,seq:cgt)
     gene            complement(2641..2859)
                     /locus_tag="VF531_11505"
     CDS             complement(2641..2859)
                     /locus_tag="VF531_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714632.1"
                     /translation="MPQVNEDFERFITTRCQAIEKQLADKPEYIEIENSLAEIGAARE
                     IDDLVIKKIVLIEELFYRKGFIDGIQNI"
     gene            complement(3082..3381)
                     /locus_tag="VF531_11510"
     CDS             complement(3082..3381)
                     /locus_tag="VF531_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714633.1"
                     /translation="MLDRPPKQEPPNPSDEKWGLLKYIISELKVGRMSNLERKDILLI
                     ILVPAAILVFAREKIISPDLAANGFYVWLVLMSYRIIIHPWLRAKSSPANKKRSK"
BASE COUNT          973 a          847 c          797 g          924 t
ORIGIN      
        1 ggaacaggat gccctactcc tgatccaacg gatgatggga aataaacgtc tgaaatccat
       61 caaccgggtt gaggtatcgg tcctcaccga actggtaaac atcctggcga actctttttg
      121 gcgggccttg accgaacagg cccagttgaa gtggtggatc actccgccca gccgggtaaa
      181 tgaactgagc cgggcgctaa catactcagc caaggtatat accttggatc atcttttgat
      241 tcatctggaa tacctgatcc cgatgatgga gatccggatt caattcacca tgctgcccac
      301 caagagtacg atcaaaaaga tactgacaaa actcaatccg atccaatata ttcatgaaga
      361 gctcgaaccg gaattaccgc tcaaggtcga accgggagcc tgatactccg ggttaattcc
      421 aggcgggact gtgcactacg gtcttgggta ttttccaacc cgtaatttga gatactaagg
      481 gaaactatcc aaggagtgat cgttttgcgt tggcgacggc ccagaatatc cattggttta
      541 ttcataattg gcatggtact gatcggctcg gcagtgatct tcaaatatac cagcgccgaa
      601 cctgcggcac ccaatcgcca gtccggagat gttgaattgc tggcccgggt gattcgggcc
      661 gaggcggagg ccgagccgtt taccgggcag gtggcggtgg gtgccgtgat tatgaaccgc
      721 atccaaaatt cgaagtttcc caagaccatt gccggtgtca tctaccaacc ttcagccttc
      781 gaatcggtaa gcaatggtga gatcaaccgt gccgccaccg attccgcccg gcgcgcggct
      841 caatcggcca tcagcggctg ggacccttcc ggaggcgccc tctttttctt taaccccgcc
      901 aaagtcgggg ctcgctctta cgtatggaca cgaaagatca ttcaacaaat cggcaagcat
      961 gtattcgctt tgtagtcaac caccggaggt ggctaaatac ctagaaccgg cggacccact
     1021 cccaaacggc aggaaaatcg gaataggcca cgaatgaata gcagaacata attttttggg
     1081 gggaattcca aatgttgttg agggaacgtc tgccggaaga tttaaaaacc gccatgaaga
     1141 acagggagac cttgcggctg tcggtgatcc ggatggccaa ggcggctgtg atgaatcagg
     1201 agatcgcacg ggggcacgaa ttaagcgacg atgaggttat tgaagtcttg gcgaaggaag
     1261 tcaagcaacg aagcgatagt attcccgaat acgagagggg aaaccgcccg gacatcatcg
     1321 accagttaaa acaggagatc gccattctta agggatatct gcccgctcaa ttgtccgaag
     1381 acgagttacg ccggattatt caggagacag tctccgcgat cggcgcaact tccaagaaag
     1441 agatgggtaa ggtaatggcc gccctgatgc ccaaagtcag aggccgcgcc gacggcaaac
     1501 tcgtcaacga gattgtcggt tccttgctcc agggctgacc ggaaaataca taccgtctta
     1561 aaaccgggta taactagaaa ataccgggaa aaaatagaac catgactgaa cgaactgtaa
     1621 aaaaggaact ccatgtccgg gtcgacccgg agttatacga gaaactccgc cagatcacct
     1681 tttacgagcg gcagaccttt agcggggttg tcaaccggtt attaatcgaa tatgtggagg
     1741 aaaaggccat tgaggagccg gagatcgttg cccctccggt tttataagat ccggaagacc
     1801 aaggcggaat aagtaaaagc ccctttccaa atcctgaaag gggctttttg actgctccaa
     1861 aaatgcttaa ccctgtttgg gggctcctac cgggcaaaca tcggcgcaag cgccgcattc
     1921 aatgcatttt ccgggatcga tgacatattt gtcggaacct tcgctgatcg cttccaccgg
     1981 gcactcggac tggcaagcgc cgcaactgat gcattcatcg ctgattttat agctcataag
     2041 gcacctccta agttttcatt gagaatcaaa aatatcgttt ttctttttga caaaaacccg
     2101 caaaatcctc ttcagttctc ctacttaatc cattattcag atttcctgaa tttcccttcc
     2161 caaagaactt gcttttttga aagggttcca ttataatata ttatgcttaa cgcaatattt
     2221 gctgacgaat tgttttcaag cgggtgtggc tcagtggtag agcatcggct tcccaagccg
     2281 aggaccgcgg gttcgaatcc cgtcgcccgc tccattcatt ttaaactatc tacttctaga
     2341 gttttctaga agttttttca tctttcttcc cgtcgcttcg gggaatactt cgtgggcgag
     2401 ataggcgcga cggagattga aatccgctac aatccatgat aaaataattt gtaggttcac
     2461 gcgatcttaa gctggcgtag ctcaatggca gagcagcggt atcgtaaacc gcaggttgat
     2521 ggttcaattc cgttcgccag ctccaatatt atcaacgaat tccgggaaac cggaattttt
     2581 tattttccga atttcaccat aatttcacca taaattccgc aaaccttcta ttcatgcggg
     2641 ttatatattt tgaataccgt caataaaccc tttccgataa aacagctctt cgataagaac
     2701 gatttttttg atgaccaaat catcaatttc cctcgctgct ccaatctccg cgagactgtt
     2761 ttcgatctct atatattccg gcttatcggc cagttgtttt tcaattgcct ggcagcgcgt
     2821 agtgataaac cgttcaaagt cctcgtttac ctggggcatt ttttatcctc ctcttaatcg
     2881 aacatatgtt cgatacttat tataccaccc gcttcccaaa ttttcagact tgactttttt
     2941 cttaatcttg tcccggcacg gaaaacaaaa attattttta cgattacgag aggcattaat
     3001 aggcaaaata taagctctaa cgccatattt agtttagttg cgcaggtttg gcgtataaaa
     3061 aataaaccct aaagggcggg tttatttcga acgcttttta ttagccgggc ttgattttgc
     3121 tcttaaccat ggatgaatta ttattcgata tgacatcaat acaagccaaa cataaaaacc
     3181 attcgcagct aaatctggcg atataatttt ctctctagca aaaactaaaa tcgcggctgg
     3241 aactaatata attaagagaa tgtcttttcg ttccaggttg gacattctcc caactttaag
     3301 ttcactgata atgtatttta aaagacccca tttttcatct gaaggattag ggggttcttg
     3361 cttcggaggt ctatctagca attttccctc ctccagcttc catattcaga aatggaagta
     3421 tgcaagaaac taatacctaa tacacatcca atgattcccg agaatattgg aattactttc
     3481 agaacagaca ataaaattgc ggcgataatg acaattatat aggctccaat gttgataatc
     3541 a
//
LOCUS       DASVMB010000406         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_274889,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000406 DASVMB010000000
VERSION     DASVMB010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274889"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF531_11515"
     CDS             complement(<1..331)
                     /locus_tag="VF531_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714634.1"
                     /translation="MVEAMDQFGAVALFLARYLLILAVSYLLAAVIFPSLIRLVKEAG
                     LVRPNFKGENIPASTGIIFLAVLPFSIFLGMILKIPAFNTINSFLFLFVAIGMGFMGF
                     FDDQLGNH"
     gene            complement(344..1009)
                     /locus_tag="VF531_11520"
     CDS             complement(344..1009)
                     /locus_tag="VF531_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7714635.1"
                     /translation="MVSILIPAYNEAKTIRDTVYAVKNLDKVDQIIVINDGSKDETSR
                     LAGEAGAEVVDLAQNLGKGGALNFGLQYARGEIIGLLDADLGKSAAEVEKLLIPVLEN
                     KADMTIGRFPPASKKGGFGMVTSLAKKGIRWFTGLEVASPLSGQRVMQRNVIESVTPF
                     ESGFGVEVGLTIDVFRHGFRVKEVPVQMQHAETGRDLAGFLHRGHQFMDVALVLVKRL
                     IKK"
     gene            complement(1125..2018)
                     /locus_tag="VF531_11525"
     CDS             complement(1125..2018)
                     /locus_tag="VF531_11525"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX7714636.1"
                     /translation="MIVDMRYHVASLVAVFLALGLGIIVGANLGVSANPQFEKQIDQL
                     EQTYQKIRNDQKTLEASLQTKDNELEIANQFQNAIVPSLVGDRLLGKRIAIIRTNDSI
                     DFKYAKQVVNLLRRAGAEVTSITSITKPFDFSDPAFKTELVEAFNLNTTETKDLFPLV
                     SSKMMQILIHGQGSSELLFLQNKDLIQLWGDYNRGYVDTLIFLGGGMNPLGDYQKELD
                     LPLLESARKSGVTVVGVEPSFVTNSYMRLYQSKCLGTVDNIDTAPGEVSLVYLLANGK
                     RGYYGVKDTARSLLPTLKLNY"
     gene            complement(2049..3170)
                     /gene="steA"
                     /locus_tag="VF531_11530"
     CDS             complement(2049..3170)
                     /gene="steA"
                     /locus_tag="VF531_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX7714637.1"
                     /translation="MEGKARARIDVRTKNLAKRIKQGEIAVIDHADLDQVSADSLVKA
                     KVRLVINASRSMTGRYPNPGPAVLLQNGIEILDNIGRPVFDKIREGDILEIRGAEIWR
                     GQELLGTGTYMDLKTINHRLREVRNNLSDELDKFLHNTLEYALKEKDLILGNLKLPDV
                     KTKFEGKPVLVVVRGQNYRQDLTTIQSYIDEVNPVLVGVDGGADALIEFGYKPHMIIG
                     DMDSVTDEALRSGAELVVHAYPDGRAPGLERIQKLNLPAKTFPAPGTSEDITLLLAYE
                     NGADLIVAVGTHSNMVDFLEKGRRGMGSTFLVRLKVGAILVDARGVSKIYQGRVKLSY
                     LVQLSLAGMLVILVVLFQTQWVREFGRTLWLQLKVLLGL"
     gene            complement(3212..3988)
                     /gene="spo0A"
                     /locus_tag="VF531_11535"
     CDS             complement(3212..3988)
                     /gene="spo0A"
                     /locus_tag="VF531_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HEX7714638.1"
                     /translation="MSGIKIGIADDNQEFCQLLKDYFATQNDLELTVIVHNGIDAIKA
                     ISQNQMDVLLLDIIMPQMDGLGVLEWLKEHQPPHRPKIIIFSAFGQEEITKSAINLRV
                     DYYILKPFDLAILTQRIFEIAKGKEDLISSRRIPHTDKDLESQVAQIIQSLRIPSYFK
                     GYSYLKDAILLTIADPNLINEVTKRLYPTIATHYNTTQFRVERAMRFAIETAWNKGNV
                     NVLHELFGYCVDDKKGKPTNASFIAKIADKIRLEKIANKN"
BASE COUNT         1190 a         1027 c          854 g         1135 t
ORIGIN      
        1 catgatttcc tagttgatca tcgaaaaatc ccataaaacc cataccgata gcgacaaata
       61 aaaaaaggaa cgaattgata gtattaaaag ccggaatttt taagatcatt cccaaaaaga
      121 tactaaaagg caacactgcc aaaaaaataa ttccggtaga ggcgggaata ttttcacctt
      181 taaaattcgg tcttaccaat ccggcttctt tgacgagccg gatgagtgaa ggaaatataa
      241 ctgcagcgag taaataagat acagccaaaa tcaacagata acgggccaag aaaagcgcca
      301 ctgctccaaa ctggtccatc gcttcaacca cagaatcacc ccgttatttt ttgatcaatc
      361 ttttgacaag taccagagcc acatccatga attgatgacc ccgatggaga aacccggcca
      421 gatcccgtcc ggtctccgca tgctgcatct gaacaggaac ttcctttacc cggaaaccgt
      481 gacgaaagac gtcaatcgtg aggccaactt ccaccccaaa cccggattca aacggggtaa
      541 cggattcgat gacgttccgt tgcatcacac gttgacccga taaggggctt gccacctcga
      601 gtcctgtgaa ccaacgaatc cctttctttg caagtgaagt gaccattcca aatccaccct
      661 ttttgcttgc aggcggaaaa cgcccgatgg tcatgtcggc tttattctcg agcactggaa
      721 tcaataattt ctcaacttcg gcagcggatt tacccagatc ggcatcaaga agtccgatga
      781 tttcgccccg ggcgtactgc agcccaaagt ttaaggctcc gccctttccc agattttgcg
      841 cgagatccac cacttcggcc ccggcctcac ctgcaagcct gctggtttcg tcttttgagc
      901 cgtcgttgat gacaatgatt tgatctactt tatcgaggtt tttgaccgcg tagaccgtat
      961 cccggatggt tttggcctcg ttataggcag gaattaagat cgataccaat gtattcctcc
     1021 tttttgttct tacgactatt tttaagccgg attttgcaaa tggaaaaaca aacgatgtct
     1081 tgcgcgcgtg cgcaacgatt agtatacatc gctttcaacg ataatcaata attcaatttt
     1141 aaagtcggca gtaaggaacg ggcggtatcc ttgacgccgt agtaaccacg ttttccattg
     1201 gccaataaat aaaccagact gacctctccc ggggcagtat cgatattatc cactgttccg
     1261 agacatttgg attgatataa acgcatataa gaattggtca caaaactcgg ttctacacca
     1321 actacagtta cccctgattt acgggccgat tcaagtaacg gtagatccag ctctttttga
     1381 taatcaccaa gcggattcat cccgccgcct aaaaaaataa gcgtatcgac ataaccccgg
     1441 ttgtaatccc cccagagttg aattaggtct ttattttgca gaaaaagcaa ctccgaactc
     1501 ccttggccgt gaatgaggat ctgcatcatc ttggaggaga cgagcggaaa aagatccttg
     1561 gtttctgttg tatttaaatt gaacgcctca accaactcgg ttttgaatgc cggatctgag
     1621 aaatcaaaag gtttggtaat ggaagtaatt gaagtaacct cggctccggc ccgccgtaaa
     1681 agattgacga cctgtttcgc atacttaaaa tcgatggagt cgttggtacg aataatggca
     1741 atccgcttcc ccagcagcct atccccaacc agactgggga cgatggcgtt ctgaaactgg
     1801 tttgcgatct caagctcatt atctttcgtt tgtaacgagg cctcaagcgt tttctgatca
     1861 tttcgtatct tttgatatgt ctgttccaac tgatcaatct gtttttcaaa ctgaggattc
     1921 gccgaaacac ctaaattggc ccccacaata atccctaaac ctaaagccag aaacacggct
     1981 accaatgaag cgacatggta tcgcatatcg acaatcatga caatccccct cttacgacaa
     2041 gaaaatcctt ataatcccaa caaaactttt aactgcaacc acaaggtcct accaaactca
     2101 cgcacccatt gagtttgaaa caaaacaacc aagatgacca acatccctgc cagggataac
     2161 tggactaaat aactcaattt gacgcgtcct tgataaattt tgctgacacc acgagcgtca
     2221 accaggattg cgccgacttt caaacgtacc aagaacgtgc tgcccattcc acggcggcct
     2281 ttctctaaaa aatccaccat attggaatgg gttccgaccg caacgatgag atcagcacca
     2341 ttttcgtaag ccaatagtag agtaatatcc tcactcgttc cgggggctgg aaatgtttta
     2401 gccggtaaat ttaatttttg aatccgttcc aatcccggtg cccgtccatc gggataggca
     2461 tgaaccacca attccgcccc ggatcgcaag gcctcatcgg taacactgtc catatccccg
     2521 ataatcatgt gcggtttata accgaactcg atgagcgcgt cagccccgcc atctaccccg
     2581 accaataccg gatttacttc atcgatataa gattgaatcg tcgtaagatc ctggcgataa
     2641 ttctgccccc gaaccacgac taaaaccggc ttaccttcaa actttgtctt gacatcagga
     2701 agtttaagat ttcccagaat caagtctttt tctttcaagg catattccag tgtgttgtgg
     2761 agaaatttat ccaattcatc cgaaaggttg ttccggactt cccgcagcct gtgattgatg
     2821 gtctttagat ccatatacgt tccggttccc aacaattcct gaccacgcca gatctcagcc
     2881 ccgcgaattt cgagaatatc gccttcccgg atcttatcaa atacaggccg gccgatgtta
     2941 tccaagattt cgattccatt ttggagtaaa acggcgggtc cggggttggg gtatcttccg
     3001 gtcattgaac ggctggcgtt gatgaccaac cgcactttcg cttttactaa tgaatcagcg
     3061 gagacctgat caagatctgc gtgatcaatt acagcaatct ccccttgttt gattcgctta
     3121 gccaaattct tggtgcgcac atcgattcga gcccgggctt ttccttccaa atgaatccct
     3181 ccgtagcaaa accgctacta tatcttctcc cttaattctt attggcgatt ttttcaagac
     3241 gaatcttgtc ggcaattttg gcgatgaatg aggcattggt aggtttacct tttttatcgt
     3301 ctacgcaata cccgaataac tcgtgtaaaa cattgacatt gcccttattc catgcggtct
     3361 caatcgcaaa tcgcattgct cgctccacgc gaaactgagt ggtattataa tgggtagcaa
     3421 ttgttgggta aagccttttc gtaacttcat taatcaggtt ggggtcggct atcgtgagca
     3481 aaatcgcatc ttttaaataa gaataacctt taaaataaga aggaatcctc aaactttgaa
     3541 tgatttgagc cacttgtgat tccaggtctt tatcggtatg ggggattctt ctggaactaa
     3601 tcaaatcctc cttacccttg gcgatctcga aaatacgttg ggtcaaaatg gctagatcga
     3661 agggttttaa aatataataa tcaacccgca ggttgattgc actcttggtg atctcctcct
     3721 gtccaaaagc ggaaaaaatg atgatcttcg ggcggtgagg gggttgatgt tctttaagcc
     3781 attccaaaac gcctaaccca tccatttggg gcataatgat atccagcaat aaaacatcca
     3841 tttgattctg ggatatggct ttaatggcat caataccatt atgcacgatt acagtcaatt
     3901 ccagatcatt ttgggtagcg aagtaatcct ttagaagttg acaaaattcc tgattatcat
     3961 cggcaatccc gattttgatt ccggacaaaa cggaaagcac ctcaattatt tggcataatt
     4021 atagattaat tttcgctacc gtagaaaaaa ctccttttaa attattctga aagtattttt
     4081 ataaactaac aggggattct tcgtgcatcc gagattgaat aaaggatatg gccaagaaaa
     4141 aaaccgactg aagagccggt ttaggaagcc ttcgggattg gggttgtcac gggagaacga
     4201 ggagtt
//
LOCUS       DASVMB010000407        12021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_419848,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000407 DASVMB010000000
VERSION     DASVMB010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419848"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..249
                     /gene="rpoZ"
                     /locus_tag="VF531_11540"
     CDS             46..249
                     /gene="rpoZ"
                     /locus_tag="VF531_11540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7714639.1"
                     /translation="MINPPLDELLKHVDSRYTLVTAAAKRARRILEQETPSEERSIKP
                     VSAALEEISAGKIKVERTKTGIK"
     gene            433..2904
                     /gene="priA"
                     /locus_tag="VF531_11545"
     CDS             433..2904
                     /gene="priA"
                     /locus_tag="VF531_11545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7714640.1"
                     /translation="MSQLIAKVLMASRAQHLDQLFDYMVPEDIVTSLQIGARVVVPFQ
                     GQLNLAYVWELTDHSDFSSLKSVRQLIDSPPLLTRTQYQLIDWLADYYFCSRADVIQL
                     CLPPGAKQTRRDGFRLAASPESIREQLISAGFQEDISQKVYQLLTAATEAQWTRSKWQ
                     KEFQALGVIWEWLLRSKLLLPITEIAKPRISSKTIRVYRWNLPALDDRNPAGIRVKNV
                     LLQHPQGMAGPELAAAAEVSAAVIKRLLNQGKIDCSLQKIERIPVGFTPVETDGTHKV
                     QFNPEQQTVLDTFKSSSSRLFLLHGVTGSGKTEVYFEAAAEALRRGLQVLYLVPEIAL
                     TPQTLARARNRFGDQVALLHSNMSDGERYDQWFKVKNGQANFLLGARSAVFAPFTKLG
                     LVIVDEEHETTYKQEETPRYHTRRVVEQLSEITGAKAIFGSATPELESFFAAQTGRYQ
                     YLELTRRYNDQSLPEVAVVDMREELRNGNRNILSRVLHQSIADNLANNEQVILLLNRR
                     GYATFILCRDCGQVLKCPSCDVALIYHANDKVLRCHYCDYRQKMPDTCPKCHSSRIRY
                     FGHGTQKLEEEMAQNFPQARLIRMDLDSTSKKGAHQEIYQKLVQGEFDILLGTQMIAK
                     GLDLPRVTLVGVITADSSLNLPDFRAAERTFHLLTQVAGRAGRGTKPGRVIFQTYNPA
                     HYALKYAQHHDYLGFYQEELAQRRELSFPPFTELFRFGFSSIRSVKVSAAAEEFCRIL
                     REQQAKIATARPHEESGLPQVIEILGPAPALIPKVQDRFRWQILVKAGEPAVLRELVQ
                     STWSLFPFRKFPEVQIFRDRNPYAI"
     gene            2993..3436
                     /gene="def"
                     /locus_tag="VF531_11550"
     CDS             2993..3436
                     /gene="def"
                     /locus_tag="VF531_11550"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7714641.1"
                     /translation="MAILMIVLEGDPVLRRKAKEISKVNKRLRNLAKDMLETMHDAKG
                     VGLAAPQVGISERLVVIDTGEESGPYILFNPKIISRTGENKDVEGCLSIPGRNEYITR
                     AEKVSVTGMNLEGRQVTVTGQGLLARALQHEIDHLDGILFIDYLT"
     gene            3454..4413
                     /gene="fmt"
                     /locus_tag="VF531_11555"
     CDS             3454..4413
                     /gene="fmt"
                     /locus_tag="VF531_11555"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7714642.1"
                     /translation="MRLLFMGTPEFAAASLEHLIRGGYDIIGAVTQPDRPKGRGMELQ
                     PSAVKTVALKHGLRLWQPMAVAAPEFLEQFTEINPDLVIVVAFGQKIPPQVLYGPKYG
                     CINVHGSLLPKYRGAAPIQWSIINGDSTTGVTTMYMDEGWDTGDIIYQETVNIGINEN
                     FTSLYKRLAELGGRLLVKTVHDIAAGTAPRSPQDSSAATKAPKPGLELTRIRWDDPTA
                     RIHNLVRALNPSPGVETIFNQERLKILESDCPADLEKSQGTSVTPPPEPGMITALIKK
                     RGILVATGDGNILVTQVQPSGKRPMLAIEYANGRRLQVEMKFT"
     gene            4494..5186
                     /locus_tag="VF531_11560"
     CDS             4494..5186
                     /locus_tag="VF531_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX7714643.1"
                     /translation="MFFDPTFLLLIPAIIFTIYAQFRVQSTFNKYQEVQAVSGMTGAQ
                     VARELLDHANLHDVPVELTPGTLSDHYDPRSRVLRLSPEVYHGHSLASLGVAAHETGH
                     AIQHANAYAPLTIRNGVFPIANIGSNLGYILFFIGLFFGGGASNFLLDLGIILFSFFV
                     FFTVLTLPVEFNASNRAMRLLTEGGYLMQGEETTGARKVLSAAAMTYVAAAATAILQL
                     VRMLLIRGNRDE"
     gene            5179..6597
                     /gene="rsmB"
                     /locus_tag="VF531_11565"
     CDS             5179..6597
                     /gene="rsmB"
                     /locus_tag="VF531_11565"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX7714644.1"
                     /translation="MSSRGRNKTDSSRKVVPLKGRTGRELAGLVLDRIVRDQAYAGLA
                     LNEIFREFPDSDQREKAFATELVYGTLRHLITIDYILGRLLSRPLASLKIPVASQLRL
                     AIFQLEYLPDIPERAVCHSAVENVKHSAFSGLAPLTNGVLRNFIRTRNQIRFPQPTEN
                     LEEYLSIRYSHPRWLISRWIRQFGPNQAEELLRLDNQSPPLTLRVNRLRAVPAAIRDG
                     LNERGIHCHAGLWLDEAVELNENAGAISELPEFLSGQVFVQDESPMLVAHLLNPRPGE
                     RIIDLCAAPGGKSTHLAELMEDKGEIISIDDHPHKVKLIASNAERLGLDCIKPQLGDA
                     REFTLSEGNLVDAVLLDAPCSGTGVLRRKPDARYRRREADIAALSRTQQELLDHAATL
                     VRPGGRLVYSTCSLETEENEGQIKSFLANHPHFIPVEYQPFLPEKLRESLFDPEAKWA
                     TVFPTAGGGDGFFLCRMERSRE"
     gene            complement(6609..7829)
                     /locus_tag="VF531_11570"
     CDS             complement(6609..7829)
                     /locus_tag="VF531_11570"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX7714645.1"
                     /translation="MKYLVILADGMADYTVPELNGKTPLECGNKPNMDQLARLGEVGL
                     VRTVPPGMPPGSDVANLAVMGYDPHQFYTGRSPLEAVSIGVDLESGDVAFRCNLVTLS
                     DHPVYEEKVMVDYSSDEITTSESGELLKSLARELNSEEFNFYAGISYRHLLVWHGGPM
                     DTGLTPPHDISDRPITTFLPKGNGASELLALMKKSQEILNNHPVNQNRITRGLRPANS
                     IWLWGQGKKPNLVHFYERFGLHGSVISAVDLAKGIGICAGLKVIEVPGATGNVHTNFK
                     GKAQAALDALREGQDFVYVHIEAPDEAGHRGEIENKIKAIEKIDAEILGTILKELPQI
                     TPEFKIMVLPDHPTPLTLRTHVADPVPFVIYQNGQPASEHSVARFDEKSAAGTGVFVD
                     EGHQLMERFLRCGM"
     gene            8175..10118
                     /locus_tag="VF531_11575"
     CDS             8175..10118
                     /locus_tag="VF531_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7714646.1"
                     /translation="MSWLKRLLIGKPLENEALSSEKYSIFWGLPILSSDAISSVAYAT
                     EEILIVLVPAVGILAFAPLTGISWAIIGLLAILTFSYRQTIQAYPNGGGAYIVASENL
                     GPYAGVAAGAALAIDYTLTVAVSISSGVFNIASAFPFLLKYQVLVALIILGLIFIGNM
                     RGIRESAKIFGIPAYAFMFAMISMIVAGIAKVKIFGYIPTEPNLSAAVEPLTIFLVLK
                     AFSSGCAALTGVEAVSNAVPNFKEPSAKNAKTVLLMLSLIILILFGGISYLANLYHVA
                     PSPTNSVLSQISSQIFGHSLMYYFVQIATMIILALAANTAYAGFPMLFSLMAHDGYAP
                     RQLSQRGERLSFSNGILVLTLFAALLIIVFKANVTSLIGLYAVGVFISFTLSQSGMFV
                     RWLRNREKGWRYKAFINGFGALVTLITAIVIGQTKFFMGAWIVIVAIPILMVIFLRIK
                     RHYMAVAYQLRLLPEELEAVDLTKETYRNHVIVPIASLNKASIRALRYAKTISSHIVA
                     FNVATSPEEERKFRERWDKIRTDIPLIVKYSPYRKVVGPLLEFIESYEEHSYKKGDMI
                     TVVLPQFTIQNWWQFFLHNQSRFSIQRRLLKHKHIVVATIPLQLRKDSEIYDGAFTFR
                     KMSKECEHGKTPGDQGTKQPRQV"
     regulatory      10240..10377
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            10472..11644
                     /locus_tag="VF531_11580"
     CDS             10472..11644
                     /locus_tag="VF531_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HEX7714647.1"
                     /translation="MKTYEEINERISQGKAVVVTAEEIIGIVAEKGCEKAAKEVDVVT
                     TATFGPMCSSGAFLNFGHADPPIRMGKVWLNDVPAYAGVAAVDAYIGATELSETKGMK
                     YGGAHVIEDLLAGKEVALRATSYGTDCYPRKEIQTFIKLSDLNQAYLFNPRNAYQNYG
                     VAVNSSGRTIYTYMGTLLPNLGVATYSSAGELSPLLNDPFYRTIGIGTRIWLGGATGY
                     VAWQGTQHNPEQTRAENGIPLGGAGTLALIGDMKEMSRDFIRAAVFDRYGVSMYVGVG
                     IPIPILDEEMVKYTAVSNRDIKTTIFDYSVPKRSRPNFGQVSYADLRSGAITINGRSV
                     STAPLSSLEKAREIAELLKERIASGHFTLTKPVERLPLSNQIHTLEIRNKEVNLNA"
     gene            11637..>12021
                     /locus_tag="VF531_11585"
     CDS             11637..>12021
                     /locus_tag="VF531_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX7714648.1"
                     /translation="MPKKKMVLTFPPELTEEPLTYHLVKDYDLGLNILKAKITPGEVG
                     KLVVEISNGTEANLQAGIEYLIGRGVTVEPVGKEIVMDESNCIHCGSCTAVCQPKALS
                     VAAPDWKLVFDKERCIVCELCVHACP"
BASE COUNT         2975 a         2921 c         3063 g         3062 t
ORIGIN      
        1 tgattatatg gcagggcagg catagaatac aagggggcac attgattgat caatccaccg
       61 ctagatgaat tactcaaaca cgttgacagt aggtataccc tggttaccgc ggctgcgaaa
      121 cgagctcgcc gtatcctaga acaagagact ccgagtgagg aacgatcgat taagccggta
      181 tcggcggctt tggaagagat cagcgcaggt aagatcaaag ttgaacggac aaaaaccgga
      241 attaaataga cactggccgg gatacggatg tggtaagatc tcagagtgcg aatgctttgg
      301 gatctttatt cttattcatc cttttttgaa ggtaagttca atctatgaca atgaacgtat
      361 catgctccgc ataggctctt ttggagtcga tggggacagg gtatcggatc cattaatacg
      421 gagcttggga aaatgagtca attgattgct aaggttttga tggcttccag ggcccaacat
      481 ttggatcaac tatttgatta catggtccct gaagatattg taacttcgct tcagatcggg
      541 gcccgggtgg tcgttccatt tcaagggcag ctcaacctgg cctatgtttg ggaattgacc
      601 gatcattcgg attttagttc tttaaaatcg gtgcggcaat taatcgactc tccgccgcta
      661 cttacaagaa cccaatatca actcatcgat tggctggctg attattactt ttgcagccgg
      721 gcggatgtaa ttcaactttg ccttcccccg ggggcgaaac aaacccgtag ggacggcttc
      781 cggttggctg catcacccga gagtatccgg gaacaactaa tctcagccgg ttttcaagag
      841 gatatttccc aaaaagttta ccagttgttg actgccgcta ccgaggctca atggaccagg
      901 tcaaaatggc aaaaggagtt tcaagctctg ggggttatct gggaatggct cctgcgtagt
      961 aagttgttgc tccctataac cgagatcgcg aaaccgcgga tctcttcgaa gaccattcgc
     1021 gtgtaccgct ggaatcttcc tgctttggat gatcgaaatc cggccggtat ccgcgtaaaa
     1081 aatgttctgc tgcaacatcc ccaaggtatg gccggtccgg aactggcggc agctgccgaa
     1141 gtaagcgctg ccgtaatcaa gcgtctcttg aatcagggta aaatcgactg ttcgttgcag
     1201 aagatcgaaa ggatcccggt ggggtttacg cctgttgaga ccgatggaac acacaaagtt
     1261 cagttcaacc cggagcaaca gacagtactt gacactttca agtcttcatc gtcccggctt
     1321 ttcttacttc atggcgtgac cggcagcggt aagacagagg tctattttga agcggccgct
     1381 gaagctctcc gccgtggcct gcaagtttta tacctggtac cggaaattgc gctcacgccg
     1441 caaaccttgg ccagggcccg caaccgcttt ggtgatcagg tggcgttgtt acatagcaat
     1501 atgtcggatg gagagcggta cgaccaatgg ttcaaggtca agaacggtca agccaatttc
     1561 cttttgggtg ctcgttctgc cgtatttgcg ccttttacca aacttggcct ggtgatagtg
     1621 gatgaagaac acgaaaccac ttataaacag gaagaaactc cgcgatatca cacccgccgg
     1681 gttgttgaac aactgtccga aattaccggg gccaaagcga tcttcggtag cgccaccccg
     1741 gagttggaat ctttttttgc cgctcaaacc ggaagatatc aataccttga gcttacacgg
     1801 cgctataacg accaatcctt accggaagtg gctgtagtcg atatgcggga ggaactgcgg
     1861 aatggaaatc gaaatatttt aagtcgtgtc ctgcatcaat ccattgcgga caatctggcg
     1921 aataacgagc aagtcattct gttactgaat cgccgtggtt acgccacatt catactgtgc
     1981 cgcgattgcg gccaggtcct gaagtgtcct tcttgcgatg tggcgttgat ctatcatgcc
     2041 aacgataagg tactccgttg ccattactgc gattatcgcc agaaaatgcc ggatacctgt
     2101 ccgaaatgtc acagtagccg gattcggtat ttcggccacg gcacccaaaa gctcgaggag
     2161 gaaatggccc agaactttcc ccaggcccgc ctgatacgaa tggatttaga ctctacctca
     2221 aaaaaaggcg ctcatcagga aatctatcaa aagctggtgc agggggagtt cgatatcctg
     2281 ctgggtacac agatgatcgc caagggcctg gatctgcccc gggttacatt ggtcggagtg
     2341 attacggccg attccagttt aaatctgcct gattttcgcg ctgcggaaag gacatttcat
     2401 cttttaaccc aggtggcagg gagagcaggc cggggaacca aaccgggccg ggtgattttt
     2461 cagacctaca acccggccca ttacgcattg aagtatgccc aacaccatga ttatttggga
     2521 ttttaccaag aagaactggc tcagcgtcgc gaattgagtt tcccaccctt taccgagtta
     2581 ttcaggtttg gctttagcag tattcgttcg gtaaaagtga gcgctgcggc ggaagagttt
     2641 tgccggatac tccgtgaaca gcaggcaaaa atagctacgg cgcgtccaca cgaagaatcc
     2701 ggcttgccgc aggtaatcga gatcctggga cccgctccgg ccttgattcc aaaggttcaa
     2761 gatcgatttc gctggcaaat cctggttaaa gccggtgaac cggccgtatt aagggaattg
     2821 gtccaatcaa catggagctt gttccctttc cgtaagtttc ccgaggttca aatctttcgc
     2881 gaccggaacc cgtatgcgat ctaacgtaaa aacatggtaa aaaagctcca gtcccataca
     2941 taatgtatta taatatttca ggagtctttt gcttgatagc gaggtgttca cgatggccat
     3001 tttaatgatt gtcttggagg gtgatccggt tttacggaga aaagccaagg agatctcgaa
     3061 agtaaacaaa cgtttgcgaa atctcgccaa ggatatgttg gagacgatgc atgacgccaa
     3121 gggtgtgggg ctcgcggctc ctcaggttgg gatctctgaa agactggtcg tgatcgacac
     3181 tggagaagag agcgggcctt atatattgtt caatcccaag attatcagcc gtactggtga
     3241 aaacaaggat gtcgaaggct gcctctcgat cccgggccga aacgagtata ttacacgtgc
     3301 ggaaaaggtg agtgtcaccg gaatgaattt agaaggcagg caggtgacag ttaccggtca
     3361 gggattactc gccagggcgt tgcagcatga gatcgaccat ctggacggca ttttgtttat
     3421 cgactattta acgtaaaaca aaggagaact accgtgcggc tattatttat gggaaccccc
     3481 gaatttgccg cggccagcct ggaacatctg atccggggcg gttatgatat tatcggcgcg
     3541 gttactcaac cggatcggcc gaaagggcgg ggtatggaac tgcaaccttc ggcagtcaaa
     3601 accgttgctc tcaaacacgg attacgactt tggcagccaa tggctgtggc ggctcccgaa
     3661 tttctggaac aattcacaga aatcaaccca gatctcgtga tcgtggttgc ctttggccaa
     3721 aagatcccgc ctcaggtttt atacggaccg aaatacggct gtattaatgt tcacggctcg
     3781 ttacttccca aataccgcgg tgccgcgcct attcaatgga gtatcatcaa cggtgattct
     3841 accaccggtg taaccaccat gtatatggat gaaggttggg acaccggaga tattatctac
     3901 caggaaactg tgaatatagg aattaacgaa aattttacca gtctttataa acgtttagct
     3961 gaattgggcg gaagattgtt ggtcaaaacc gtccatgata tcgctgccgg tactgccccc
     4021 cgcagtccgc aagattcatc agccgcgact aaagctccca aaccgggatt ggagttgacc
     4081 cggattcgct gggacgaccc tacggcccgg attcacaacc tggtccgggc gctcaacccg
     4141 agtccgggtg ttgaaactat ttttaaccag gaacgtttaa agatcctaga atctgattgt
     4201 ccggcagatt tggaaaaaag ccagggtact tccgtcacac cgccgccgga accgggaatg
     4261 atcacggcgc ttattaaaaa acgaggtatt ctggttgcga cgggtgacgg gaacatcctg
     4321 gtcactcaag tacaaccctc cggtaaacgg ccgatgttag ctatcgagta tgccaacggc
     4381 cgcagattgc aagtggaaat gaaatttaca taatacagag gtcttcgaat ttcttcccgt
     4441 gaattgaata ccgcaatttt acctacaata tctctataag gaggatacgc gtcatgtttt
     4501 ttgatcccac atttctgctg ctgatcccgg ccatcatctt taccatctat gcccagttca
     4561 gagtgcaatc aacctttaat aaatatcagg aggttcaggc ggtttcagga atgaccggag
     4621 cccaagtggc ccgtgaactg ttggatcacg ccaatctgca cgatgttccg gtggaactga
     4681 caccgggtac tctttcggat cattacgatc cccgcagccg ggtgcttcgc ctctcacctg
     4741 aagtctacca tgggcattca ctggcttccc tgggagtggc ggctcatgaa accggtcatg
     4801 ccattcaaca tgccaatgct tacgccccgc taaccatcag aaacggggtc ttcccgatag
     4861 cgaacatcgg atccaacttg ggttatattc tctttttcat cggtctattc tttggcggcg
     4921 gcgcctcgaa tttcttattg gatttgggta tcattctctt cagtttcttt gtcttcttta
     4981 ccgtactgac ccttccggta gagttcaacg ccagtaaccg ggcaatgcgc ctattgactg
     5041 aaggcggtta cctgatgcaa ggtgaggaaa ccacgggggc acgtaaagtg ttaagtgccg
     5101 cagccatgac ttatgtggcg gctgctgcca ccgcaatttt gcaactggta agaatgctcc
     5161 tcatacgggg caaccgggat gagtagtaga ggccgcaata aaacagacag ttcccgcaag
     5221 gttgttccct taaaagggcg gaccggaagg gaattggccg gattggtttt ggaccggatc
     5281 gtacgcgatc aggcatatgc cggtttggcc ttaaacgaga tcttccgtga atttcccgat
     5341 agcgatcaac gggagaaggc ttttgccacc gagctggttt atggaacact gcgacatctg
     5401 ataaccattg attatatttt gggacggcta ctcagccgtc ctttagcgtc gttaaaaata
     5461 ccggtcgcaa gtcaactgcg cttagccata tttcaactgg aatacctacc ggacattccg
     5521 gaacgtgcgg tctgccatag tgcggtggag aacgtgaagc attcggcatt ttccgggttg
     5581 gcgccgctta cgaatggagt attgcggaat tttatccgta ctcgaaacca gatccgtttt
     5641 ccccaaccca ctgaaaattt ggaagaatat ctttccatcc ggtattctca cccgcgctgg
     5701 ctcatttctc gctggatccg ccaattcggc ccaaatcaag ccgaggagtt gctaagactg
     5761 gataaccaat ctccaccgct gactttgcgg gtaaaccggt tacgggccgt tcccgccgca
     5821 attcgcgatg gattgaacga acgggggatt cattgccacg ccggactttg gttggatgaa
     5881 gcagtagaac tgaatgagaa tgccggtgcc atatcagaat tgcccgaatt tctctccggc
     5941 caggtttttg ttcaggatga gagtccgatg ttggtcgccc atttactcaa tcctcgtccc
     6001 ggagaacgaa tcattgacct ctgtgccgcg ccgggtggaa aaagtactca tctggcggaa
     6061 ctcatggagg ataagggtga aatcatcagt atcgacgatc atcctcataa agtgaaactg
     6121 attgcgtcca atgccgaacg gctcggactt gattgtatta agccacaatt gggggatgcc
     6181 cgggagttta ccttgtctga gggaaattta gttgatgcgg tattactgga tgcaccctgc
     6241 tcaggaacgg gagtcctgcg tcgcaagccg gatgcgcgtt accggcgccg ggaagcagat
     6301 attgctgcgc tctcacgaac tcaacaagaa ctattggatc atgccgctac tcttgtcaga
     6361 ccgggaggac ggctggtcta cagcacttgt tcgttggaaa cggaggagaa cgaagggcaa
     6421 atcaagtcgt ttctggcgaa tcatccccat ttcattccgg ttgaatatca accgttcctg
     6481 cctgaaaaac ttcgggagag cctgttcgat ccggaagcca agtgggctac ggtttttccg
     6541 actgccggtg ggggagacgg ttttttcttg tgccgaatgg agaggagcag ggagtagtta
     6601 tttgtgactc acatcccaca tctcaagaac ctctccatca attgatggcc ttcatcaacg
     6661 aaaactccgg tcccggcggc gctcttctca tcaaaacgtg ccacggaatg ctcagaggca
     6721 ggttgcccat tttgatagat cacaaacggt accggatcag ctacatgagt tcggagtgtt
     6781 agcggggttg ggtgatccgg tagtaccatg atcttaaact ccggagtgat ttggggtaat
     6841 tccttgagaa ttgttccgag aatttcggcg tcaatcttct cgatggcttt tattttattc
     6901 tcaatctccc cgcgatggcc tgcttcatcg ggtgcttcga tatggacata gacaaaatcc
     6961 tgcccctccc gtaaagcatc aagcgccgct tgggcttttc ccttaaaatt ggtatgaaca
     7021 tttccggtgg cgccgggtac ctcgattact ttcaacccgg cacagatgcc tatacctttc
     7081 gccagatcaa ccgcagagat taccgatcca tgcagaccga atcgttcgta gaaatgaaca
     7141 agattcggtt ttttgccctg cccccataac cagatggagt tggccggccg caacccccgg
     7201 gtgattctgt tttggttgac cggatgattg tttaaaattt cttggctctt tttcatcaat
     7261 gccaacaact cggaagcgcc gtttcctttg gggagaaagg tggtaatcgg acgatccgaa
     7321 atatcgtgag gcggggtcaa tccggtatcc atcggaccgc catgccagac taaaagatgg
     7381 cggtagctga tcccggcata gaaattgaat tcttccgaat tgagctcccg agccaaactt
     7441 ttcagcaact cccctgattc ggaagtggtg atctcatccg aactgtaatc gaccatcacc
     7501 ttctcctcgt aaaccgggtg atccgaaaga gtcaccagat tacagcggaa cgccacatca
     7561 ccggattcca gatccactcc gatactcacc gcctcaaggg gtgaacgtcc ggtataaaat
     7621 tggtgcgggt cataacccat cactgccagg ttggccacat cgctcccggg aggcattccc
     7681 ggtggtacgg ttcgtaccag acctacttcg cccagacgtg ccaattggtc catattgggc
     7741 ttatttccac attccagtgg agtcttgccg ttcaattccg gaactgtata atccgccatg
     7801 ccatcggcta aaatgactaa gtatttcaac tgaggatcac cattccttga ttcgcaaaat
     7861 catttacata tgtttacata tgaatgaaac ttcgtttatt atatcatttg gtagcggacg
     7921 gctcaatcgt ttttggggag aaattggtgg ttcggggaag atcatacagc ataccgtaga
     7981 gtacgcttaa accgacgttt cccgaaggtt acgttttggt taaactttga atcttcttcc
     8041 tggcttttca aatcaaacca ttgcactagt ctacggtttt tgctaatata aatagcatgt
     8101 tcttcaagaa attggataaa aaagtcagac agttcatggt tcataacgaa ctgttgacca
     8161 agaggtgaag ggcaatgtct tggcttaaaa ggttattgat cggcaaaccg cttgagaatg
     8221 aagcgctctc cagtgaaaaa tacagtattt tttggggttt acctattctt tcgagtgacg
     8281 ccatctcttc ggttgcatac gccaccgaag agattcttat tgttttagtt ccagcagtcg
     8341 gaatactggc gtttgcaccg ctaaccggaa tctcctgggc catcatcggt ttactcgcga
     8401 ttttgacttt ttcgtaccgc caaaccatac aggcataccc aaatgggggc ggcgcctata
     8461 tcgtcgcttc cgagaatctg gggccgtatg ccggagtagc agccggtgcc gctttggcca
     8521 tcgactacac ccttacggta gcggtaagca tttcgtccgg tgtctttaat attgcttccg
     8581 cctttccatt ccttctaaaa tatcaagtac ttgtggcctt gatcattctg ggacttattt
     8641 ttatcggcaa tatgcggggg attagggaat cagccaaaat attcgggata ccggcgtacg
     8701 ccttcatgtt cgcgatgata tccatgattg ttgccggcat tgccaaggtt aagatcttcg
     8761 gttacatccc taccgaaccg aatttatcgg ctgccgtgga accgcttacc atcttcttgg
     8821 tattgaaggc gttttcttcg ggatgtgccg ctttaacggg tgtagaagcc gttagcaatg
     8881 ccgttccgaa ttttaaagaa ccatcggcca aaaacgccaa gacggtttta ctcatgcttt
     8941 ctctaattat tttgatctta ttcggaggca tttcgtatct ggccaatctt tatcacgtgg
     9001 caccatcgcc taccaattcg gtactctccc agatcagcag tcagatcttc ggacatagct
     9061 taatgtatta ttttgtacag attgctacca tgattattct ggctctggct gccaataccg
     9121 cctatgccgg ttttccaatg ctcttttcat tgatggccca tgatgggtac gcgccccgcc
     9181 aactttcaca gcgtggcgag cggttgagtt tctccaacgg catcctggtc ttaaccctat
     9241 ttgccgcctt attaatcatc gtttttaagg ccaatgtaac cagcctgatc ggcctctatg
     9301 ccgtaggcgt ttttatctct tttacgctct cccagtcggg aatgttcgtc cgttggctgc
     9361 gcaatcgtga gaaaggttgg cgttacaagg cgtttatcaa cggcttcgga gctttggtta
     9421 ccttgatcac cgctatcgtt atcgggcaga ccaagttctt tatgggcgcc tggatcgtta
     9481 tcgtggcaat ccccattttg atggtgatct ttctccggat aaagcgccat tatatggcgg
     9541 tggcttatca gttacggctt ctgcccgaag agttggaagc ggtggatctc accaaggaaa
     9601 cctaccgcaa ccatgtgatc gtgccgattg ccagtctaaa taaggcgagt attcgggctt
     9661 tacgttacgc caagaccatc tcgagtcata tcgtcgcctt caatgtagcc acctcaccgg
     9721 aagaagaacg aaaattccgg gagcgttggg acaaaatccg caccgacatt cctttgattg
     9781 tcaagtactc gccctaccgc aaggttgtcg ggccgttact tgaatttatc gagtcttatg
     9841 aagagcattc ctataaaaaa ggcgatatga tcacggtggt tctaccacag tttaccattc
     9901 agaattggtg gcagttcttt ctgcataatc agagccggtt ttcgatccaa aggcgtttgc
     9961 taaagcataa gcatattgtg gtggcaacca taccgttaca actcagaaag gattccgaga
    10021 tttacgatgg agccttcact ttccgaaaaa tgtcgaagga gtgcgagcac ggcaaaaccc
    10081 cgggagatca ggggacgaag cagcccaggc aagtctgatg aaccgtcgag ttgatagaaa
    10141 atctttcctt gacagtcctt tgacgtaatg gtacactatt aaaaaatcag tcaaggggtt
    10201 ttagtcttta ttttcactac aattatatag ccatagcatc actcatccag agaggtggag
    10261 gggactggcc cggtgaaacc tcggcaacct tcgtacgatt acgagtatgg tgccaaatcc
    10321 agcaggttgg tgacgaatta acggtccgcc gttaattcga atcctgaaag atgagaggct
    10381 tccgatccgc ctcttaatca aaagaggctt tttttatccg gcaaagggtg agccggtcgg
    10441 gagagatttc gggaccgagg agagtgaacg gatgaagacg tatgaagaga tcaacgaacg
    10501 gattagccag ggcaaggcgg tagtggtaac cgcggaggag atcatcggga tcgtggcgga
    10561 aaagggatgt gagaaggccg ccaaggaagt tgatgtagtg actaccgcga cttttggacc
    10621 gatgtgctcc agcggcgctt ttttaaattt cggccatgcc gacccgccga ttcgcatggg
    10681 taaagtctgg ttgaacgatg ttccggcata tgctggagtt gctgccgtcg atgcctatat
    10741 tggagccacg gaactttccg agaccaaagg gatgaagtac ggcggcgccc atgtgattga
    10801 ggatctattg gcgggaaaag aggtagccct ccgggcgact tcttatggaa ccgattgcta
    10861 tccccggaaa gagatacaga cctttatcaa attaagcgat ctaaaccagg cttatctctt
    10921 taaccccagg aacgcttacc aaaattacgg tgtagcggtg aattcttccg gcaggacgat
    10981 ctatacttac atgggaacct tacttcctaa tctgggtgtg gccacttact ccagcgccgg
    11041 agaattaagc ccgttattga atgacccgtt ttaccggacg atcgggatcg gcacccggat
    11101 ctggctggga ggggccaccg gttatgtggc ttggcaggga acgcaacata atcccgaaca
    11161 aactcgggca gagaacggca taccgctggg tggagccggc accttggcct tgattggcga
    11221 catgaaggag atgagccggg atttcatccg ggcggcagta tttgatcgtt acggagtcag
    11281 tatgtatgtg ggggtgggga tcccaattcc tattcttgac gaggagatgg tgaaatacac
    11341 tgcggtttcc aaccgcgata tcaaaaccac catttttgat tacagcgtac ctaaacgttc
    11401 ccggcccaat ttcggtcagg tcagttacgc cgatctgcgt tccggtgcga tcaccatcaa
    11461 cggcagaagc gtctctaccg cgcccctttc ctcgctggaa aaggccaggg agattgcaga
    11521 attactcaag gaacggattg catcaggtca ttttaccctg acgaagccgg tcgagcggtt
    11581 gcccctttct aaccaaatcc atacccttga gatccggaat aaggaggtta acctaaatgc
    11641 ctaaaaagaa aatggtactc acatttcctc cggaattgac ggaagaacca ttaacctatc
    11701 acctggttaa agattacgac ttgggactca atatactcaa ggctaaaata actccgggag
    11761 aagtaggcaa gctggtggtg gagatctcaa acggtaccga agctaacctc caggcgggaa
    11821 tcgaatatct gataggccgg ggggttacgg tcgagccggt cggcaaagag atcgtcatgg
    11881 acgaatcgaa ttgcatccat tgcggttcct gtaccgcggt atgccagcct aaagcactct
    11941 ctgtcgcagc tcccgattgg aagcttgtgt ttgacaaaga acgctgtatc gtttgcgagt
    12001 tgtgtgtcca tgcctgcccg a
//
LOCUS       DASVMB010000408         9129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_197317,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000408 DASVMB010000000
VERSION     DASVMB010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197317"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VF531_11590"
     CDS             complement(<1..241)
                     /locus_tag="VF531_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX7714649.1"
                     /translation="MLDRYFDRSDFIKLTSTVVSGIFLAISWSGWLKGILPFDPAWVA
                     IVLSGAPILYVAFDGIIKRFDITAGLLVSIALIAAA"
     gene            complement(261..545)
                     /locus_tag="VF531_11595"
     CDS             complement(261..545)
                     /locus_tag="VF531_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX7714650.1"
                     /translation="MVVNQLEGHQHNKEIMDRLSRIEGHIRGIKKMLEEERPCDEVLI
                     QFSSVQAALAKTSKLLLTEHFDHCIMANLNDKTKEEELLSFKRALKKFMK"
     gene            complement(929..1004)
                     /locus_tag="VF531_11600"
     tRNA            complement(929..1004)
                     /locus_tag="VF531_11600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(969..971),aa:Ala,seq:cgc)
     gene            complement(1396..2256)
                     /locus_tag="VF531_11605"
     CDS             complement(1396..2256)
                     /locus_tag="VF531_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX7714651.1"
                     /translation="MENRLNELSGSEWLYWTNTIYETSFPPDCTHNFRKMHGAMKPPE
                     FMAEIVSFFSKSGETILDPFAGVGGTLLGAELVNRKALGMELNPCWVAVYQNICREFK
                     ISKGSLVTSEEAGQAIGSQMLLGDCFTLMTDIDENSIAAIITDPPYGCHHGATGFKAE
                     THFNMESVDPRDIGNSRDYHEYLHKMRVFGAEAYRVLRNGRYLVMLIGDRLFEGEYLP
                     LGFKVAEVLREVGFKWKGIRIWWNKATQRPLRPYAVKACFIPNITHQTLIIMRKETVP
                     KGKMKQTKSG"
     gene            complement(2498..3988)
                     /locus_tag="VF531_11610"
     CDS             complement(2498..3988)
                     /locus_tag="VF531_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714652.1"
                     /translation="MSSPVFAADLNSDPSIIDEATAEPNPTADSSAAGKSGRVAKEST
                     STGVIKLENDQIQLVAARVNYEGSGGIAEASGDVILTVNQFNLRLETDRLTYDPGNGE
                     ITIPGKVHLVSAQADLMVDTFHYNVKTETGTGGAIHGTILDVPRNFLISGKSLQVQNG
                     VVTFREAELTRCIYPTSEYVLMVRQASLDKDVLYLHGIFLKFLGVPVGYYPSYKIKTG
                     QDGIENPLSLSLGYNTDDGVVLNFSVTPQLNDNLDWSANGTLSSQGDSLVGAGLGVHL
                     NEYLADRVNLGYDFQKSSFIVFNTINLNYRHPLANLMFNYDHYFYGGDDNLSFSVARD
                     GGMIGAQLDGMREFSSTAENQLGLTINRKFQRGWLGDWQYGIMARHVSKLDETGYEYG
                     GTYGGYHVDFRPNPYLTLSYLRLDSYSGGDYRDFEPNLGSSGIYNISLPLKQKLTLGI
                     GGTYNFTDAAWKSRIYQLNYASDCYSIQVGWDTVTGAWNLTPTFNF"
     gene            complement(4075..5010)
                     /locus_tag="VF531_11615"
     CDS             complement(4075..5010)
                     /locus_tag="VF531_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714653.1"
                     /translation="MLRRYFHLLLMIVLLTVLANTGLVQAESDPMNDSLTPLEHLESL
                     EKNLIGQPARGGLVDRLGSMEELMVGRLREGSLNERLGYLETLIYTNQPHDISLLYKL
                     QALEWVLYRSETDAPFIVRLENLEKTVFGTVYSGAVTKRLERLIVQVFPEGTVKGHWL
                     SVPEGLLFKVRVLNELSSVKNYPGDSFQFQTTDTLSFNGLVIFPKGVIGKGVLKEIRH
                     PDNLGRDAQLMLDFIELRALDTTPVPVFYGSKALAMDKSRRLAVGASAAGMMAFGPGG
                     ILLGLMVKGKETVIPKGTEFYLQVREPVRIYTMEQ"
     gene            complement(5038..6423)
                     /locus_tag="VF531_11620"
     CDS             complement(5038..6423)
                     /locus_tag="VF531_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714654.1"
                     /translation="MIQTRFMSLLRLGVWIGILAGLTCFPNFAVFAQTDPSAMESEGW
                     VYQDLELLVSAGLISAYPSEWIRSGNLLSRYEIAYYLKQTVVQGLDASSPGVISPQIA
                     EALQRLMLRFQEELADLGIYAKDIHQVTPNLSSAVIPNDSYQDLDILLGKVDKPGQPF
                     YYLGQYYDEAQRKSFFFIPEIYVQGKVTRLLEGPGDSLSTLYQPQVGDVSSFLVVKGT
                     LPDGRESLKGYYLFPLQGDAVNSFSGNQSANYGLLLSLLNEVNQIGRIDTLWRYDGLM
                     NLDGYLKQRLDLQNQIFGGTIDQGLQIGSLLISTNNPSNKRNYDLNGFGLPFKHQFPN
                     MGSSEVDLDLLEGKSLQAWQINIQGSMNLSPQASIYGGLDLLYRDMNHGEESVLPSDT
                     KASAGLSYHLNDDWTILSYQSLINSELNPKWLSTTSFGVEYNDWVTLWLAYQFLKFDD
                     PVITGALSFRF"
     gene            complement(6448..6819)
                     /locus_tag="VF531_11625"
     CDS             complement(6448..6819)
                     /locus_tag="VF531_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714655.1"
                     /translation="MAVVNDEAIVFKRVIVSTDRLMATATSLCSEYPIDSVIVGDKTN
                     SKQVRRSLAALGLPLKSIDEHNSSREGRNRFLKDHTNGLARLIPIGLRSPDRPFDDYV
                     AVVLAERFFRLRNQGTSACVE"
     gene            complement(6874..8070)
                     /locus_tag="VF531_11630"
     CDS             complement(6874..8070)
                     /locus_tag="VF531_11630"
                     /inference="COORDINATES: protein motif:HMM:NF022727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HEX7714656.1"
                     /translation="MMFGILIIMIMIAFGGFIALLGDRVGMRVGKKRLSLFGLRPKYT
                     SMIITVLTGFLIAGGTLLILTAMSEYVRTAIFELNAIRNKVVSLTRELGLKENQYNQL
                     HLKLQDVAAQRNLAEDRLRKTQAQYSQAELNLQSRKAELAAVQDRLKSLTRMNHDLTN
                     QNSSLTLQEARLNQQIQNLEGWMKSLEERNKVMLDQPLLFYVGEIMTARVVEPGIPKD
                     KILSSVVEPMLNEANDLALKRGARIPGKKDVALRSLPQRISEICDQLATLTTKSVLRV
                     VVDKNSTGGEPATVNLELYPDRLIFRDGEIIEESQVSATASESELRDQLIGLLLLANN
                     KAIEKGIITDGSNLRDLVSVSEVAQIINRIKDHKTGNFKVCLVAAADLYRVDAFKVKY
                     LLVSKE"
     gene            complement(8199..>9129)
                     /locus_tag="VF531_11635"
     CDS             complement(8199..>9129)
                     /locus_tag="VF531_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7714657.1"
                     /translation="LVLMIPQNIMWGGTIAVLLATLLGLGKLTGNSETIAMRAGGMSF
                     RKVATPVLLIGLSVSILGVLLNEYVVPLSFRIADDMQAKLMSQELRSTIYNFSLNLYE
                     NDGNLKKYIYAYKYDPQLQQLQKVIIVEADHGQLLRTIEAEKMDWNGDSWFFQRGRIN
                     ELGKGSLYPITVDRAKVRYPLNLTPSDIEQRQLPPEERSISELSRYIKNVVNQGPEKA
                     KLLIYLHTKFSIPFVSLVFALLGVPLALRPQRRSSAAGYGLCFLFILAWWLLYMVGTS
                     LGRAGTINPILGAWLGDIVLAAYGVYVFTTVKI"
BASE COUNT         2550 a         2402 c         1875 g         2302 t
ORIGIN      
        1 ctgccgcggc aatgagcgca atgcttacca aaagcccggc ggtaatatca aaccgtttga
       61 taattccatc gaaagccaca tataaaatgg gagcaccgct tagaacaatc gccacccaag
      121 ccggatcaaa agggagaatc ccctttagcc aaccagacca actgatggcc agaaaaatgc
      181 cggaaacgac ggtagatgtc aactttatga aatcactcct gtcaaaatac ctatccaaca
      241 tcgcccatca cctatcacat ttatttcatg aactttttca aggcccgttt gaaactcagc
      301 aactcttcct ctttcgtttt atcgttaagg ttcgccatga tacaatggtc aaagtgctca
      361 gtgagcaata acttgctggt tttggcgagc gcagcctgga cggaggagaa ctgaatcaat
      421 acctcatcgc aaggccgttc ttcctccaac atttttttga taccccggat atgcccctct
      481 attcgcgaca accggtccat gatttcttta ttatgttgat gcccttccaa ttgattcacc
      541 accatttaca catcctatcc ccccggaggg gatgcatcta tattatcaaa tttctccttt
      601 atcttcaagc tggcataaaa aaaccatcca ttaaggacgg ctttgttcag aagccaaaat
      661 aaagctctta aaacagcacc cggtctttga ggatagggtt tccttttcga caaattgggc
      721 gccagtttga cgaaaattat cactctcaac ccctcctact gatcaacacc aaaaatactc
      781 atcaaagtaa ggggcctgat aaataccgta gtagaaatag gctcagactt tgcttttatt
      841 ttcaccctta ttcaaacttc acacttaaga aattcaaaac ccaataaaaa aacccgcagc
      901 atctgctacg agttttgaat ttcttatttg gtggacccga agggattcga accctcgacc
      961 tctgcgatgc gaacgcagcg ctctcccaac tgagccacga gcccgttaaa aaaataaaaa
     1021 gcaatgaaaa tccatgaaaa aaagcccctc cgggctttaa cgatacaagc atgaaccgcc
     1081 gaaatactca tcccatttct tgcagcggcg aagctccttt aaagtcctga tatccgaatc
     1141 cgcccgacaa ctttcacgtt ctttaatgaa tttatcgcaa acactacaaa ctttcaggac
     1201 ttcttcctga gaaagattaa agaccatctt ccgcacctcg catttgttca tgcgttgaat
     1261 gtcaaagtag gtccttcgtg ttgcaaattc attataacat gttctatcct ttttgccaag
     1321 cctgaatgag tcgtttgtca aaaaatgctt tttccggcaa cttctgaaac ctcctttcca
     1381 gaagtgaaat cttttttacc cggacttggt ttgtttcatc ttccccttgg gtaccgtttc
     1441 tttcctcata ataataagag tctgatgggt aatgtttgga ataaagcagg ctttgacggc
     1501 gtatggacgc aaaggacgtt gggtcgcctt attccaccag atccgtatcc ccttccattt
     1561 aaaacccact tctcgtaaaa cttcggccac cttaaagcca agcggaaggt actccccctc
     1621 aaataaccgg tccccgatca gcattaccag ataacggccg ttccgcagga cccggtatgc
     1681 ctcagcccca aataccctca tcttatgtaa atactcgtga taatcccggg aattcccgat
     1741 atcgcgcgga tccacggatt ccatgttaaa atgggtttcg gctttaaatc cagtggcgcc
     1801 atgatgacaa ccatacggcg gatcggttat aatcgcggca atggaattct catcaatatc
     1861 cgtcatcaac gtaaaacagt ctcccaacaa catctgagaa ccgatggctt gacctgcctc
     1921 ttcggaggta accagggagc ccttacttat tttaaactcc cggcagatat tctgataaac
     1981 cgccacccaa caaggattga gttccatacc caaggccttt ctattcacca actcggcgcc
     2041 cagtaaggtc ccgccgacgc cggcgaaagg gtcgaggatt gtctcgccgc ttttggagaa
     2101 gaaactgaca atctcggcca taaactccgg tggtttcatg gccccgtgca ttttacgaaa
     2161 attatgggta cagtccggag gaaagctggt ctcataaata gtattggtcc aatatagcca
     2221 ttccgaaccg ctcaactcat ttaaccgatt ttccaactca cccaatccca tcttctattg
     2281 atttataccc atttgccatc cacccattaa acagtaaccg gcataagtta ccttttgttt
     2341 aatcacgctc acactcggtt tattgaaagc aacttaagaa atacgaatca aacaaaaaaa
     2401 ccttccctat gtgatggaag gtttttctac tgctatcaat ccattgtacc cctggaaacc
     2461 ctattttatg acctcaatat cattaatttt gcccgactca aaaattaaac gtcggcgtca
     2521 gattccaggc accggttacc gtatcccaac ccacttgaat ggagtaacag tcggaagcat
     2581 aattcagttg atagatacgg gacttccagg ccgcatccgt gaagttgtat gttcctccaa
     2641 tccccaaagt aagcttttgc ttgaggggca aactaatgtt ataaataccg cttgatccga
     2701 gattaggttc aaagtcccgg taatccccgc cggaatagga atcaagccgc aaataactca
     2761 aggttagata ggggttgggt ctaaaatcaa catggtaccc accgtaagtc ccgccgtatt
     2821 catagccggt ttcatccaac ttggaaacat gccgcgccat aatcccgtat tgccagtcac
     2881 ccaaccatcc ccgctgaaac ttccggttaa ttgtcaatcc taattgattc tccgcggtgg
     2941 acgaaaactc tcgcatccca tccaattgag cccctatcat gcccccatct ctggcgacgg
     3001 agaaactcag gttatcatca ccgccataaa aatagtgatc ataattgaac attagattgg
     3061 cgagaggatg gcgatagttg aggttgatag tgttgaacac aataaaggaa cttttttgaa
     3121 aatcataccc cagattcacc cggtccgcca aatactcatt caaatgtacc cccaaacctg
     3181 ctccgaccaa agaatctcct tgggaagaaa gagttccgtt ggcagaccag tccaaattat
     3241 cattgagctg gggtgtaacc gaaaaattaa gaaccactcc atcatcggta ttatacccaa
     3301 gcgaaagact gagtggattc tcaatcccat cttgcccagt ttttatctta tagctggggt
     3361 aatatccgac aggcaccccc aagaatttta ggaagatacc gtgtagataa aggacatcct
     3421 tgtccagact cgcctgacga accattaaaa catattcaga tgtaggatat atgcaacggg
     3481 tcaactccgc ctcccggaac gtcacgactc cattttgaac ctgcagactt tttccggaga
     3541 tcaaaaaatt acgcggtaca tcaagaatag ttccgtgaat cgcccctccc gtcccggtct
     3601 cggtcttcac gttataatga aaggtatcaa ccattagatc agcctgagcg ctaaccagat
     3661 gcaccttacc gggaatcgtg atctcaccgt tacccggatc ataagtcaat cgatccgtct
     3721 ccaggcgtag attgaattga tttacggtca agattacatc tccacttgcc tccgctatac
     3781 cgcccgatcc ttcatagttt actcgggcgg cgaccaactg aatttgatcg ttctctagtt
     3841 tgatcactcc ggtagacgtg gactccttag ccactctacc agattttccc gcggctgagg
     3901 aatcggccgt tggattaggt tcggccgtag cttcatcgat aatcgatgga tcggaattaa
     3961 gatccgcggc gaaaaccggg gagctcatcg ttccaatcaa tagaaagatc gaaatgaaaa
     4021 tcagatatga ggtgatttta agctttataa taatggggcc acctccacta aatgctattg
     4081 ttccatggta tagatacgga caggttccct tacctgaaga tagaactcgg tccccttcgg
     4141 aattaccgtt tctttaccct taaccatcaa tcccaacagg atcccaccgg gtccaaaggc
     4201 catcattccg gcagcactgg ctcccaccgc aagtctacgg gatttatcca tggccagggc
     4261 ctttgaacca taaaatacag gcaccggggt agtatctaat gcccgaagct caataaagtc
     4321 aagcattaac tgggcatcac gacccagatt gtccggatgt cgaatctcct tcaaaactcc
     4381 tttaccgata acacctttgg ggaaaatcac caagccattg aaggacaagg tgtcggtggt
     4441 ttgaaactgg aaactatccc ccggatagtt ctttactgaa cttaattcat tcaagacccg
     4501 gactttaaat aagagacctt cgggaacgct aagccaatga cccttgactg ttccctcggg
     4561 aaaaacctgg acaattagtc tttcgaggcg cttggttact gccccggaat aaaccgtacc
     4621 gaagaccgtc ttttccagat tttctaggcg aacgataaaa ggtgcgtccg tctccgagcg
     4681 gtataacacc cattccagtg cttgcaactt atagagtaac gagatatcat gcggctgatt
     4741 ggtatagatc agtgtctcca gatacccgag gcgttcattc agagatccct cccggagcct
     4801 tcctaccatt aattcctcca tactccccaa acgatcaacc aaaccgcccc gggccggttg
     4861 gccgataaga ttcttttcca aagattcaag atgctcaaga ggggtaagtg aatcgttcat
     4921 tgggtctgat tcagcttgaa ccagacctgt attggccaaa accgtcaata acacaatcat
     4981 caataaaaga tggaaatacc gcctgagcaa atccaagacc ccctccgtga aaaagcccta
     5041 aaaacggaaa gataaagctc cggtaattac cgggtcatcg aacttgagaa actgataggc
     5101 aagccataaa gtaacccaat cgttgtattc caccccgaat gaggtcgtac taagccattt
     5161 cggattcaac tccgaattta tcagggattg atatgataaa atcgtccaat cgtcatttaa
     5221 atgataagat aaaccggcgc tggccttggt atcggagggc aaaacgctct cctcaccgtg
     5281 gttcatatcc cggtataata gatccaatcc gccataaatt gaagcctgcg gggaaagatt
     5341 catcgaaccc tgaatattga tttgccaagc ctgtaagctt ttaccttcca gtagatctaa
     5401 atccacctcg gaactcccca tattggggaa ttgatgtttg aacgggagcc caaaaccatt
     5461 aaggtcgtag ttccgcttat tggacgggtt attggttgaa atcagcagac tcccgatttg
     5521 gaggccctga tctatggtac cgccgaagat ttgattttgc agatcaagcc tttgtttcag
     5581 gtatccgtct aggttcatca atccgtcata gcgccatagc gtatcgatcc ggccaatttg
     5641 attgacttca ttcagtaaac tgagtagcaa gccataattg gcggattggt tcccggagaa
     5701 tgaattcaca gcatccccct gtaggggaaa taaataataa cccttcaaag attcccggcc
     5761 atcaggcagc gtccctttga ccactaaaaa cgaactgaca tcgcctactt gcggttgata
     5821 tagagtgcta agggagtcgc ccggtccctc taaaagcctt gtaaccttgc cttgaacgta
     5881 gatttcggga ataaaaaaga acgactttcg ttgcgcttca tcatagtact gtccgaggta
     5941 gtaaaacggt tgaccgggtt tgtccacttt cccgaggagg atatcgagat cctggtaact
     6001 gtcattagga ataactgccg atgaaagatt gggggtcacc tgatgaatat cttttgcata
     6061 gatgccaaga tcagccaatt cttcctggaa tctcaacatc aaccgttgga gggcttccgc
     6121 aatttgaggt gagattaccc caggggacga ggcatccaac ccctggacca ccgtttgttt
     6181 tagataatac gcaatctcat accgcgagag tagattaccg gaacggatcc attcggaagg
     6241 gtacgcacta attagtccgg cactaactaa taactccaga tcctgataaa cccatccctc
     6301 ggactccatg gcactcgggt ccgtctgagc aaaaacggca aaatttggga aacaggtcag
     6361 acccgcgagt atgccgatcc aaacccccaa cctcaacaat gacataaaac gagtctgaat
     6421 cattcgatcc ttcctcttgc ttgaatccta ttcaacacag gccgaggttc cttgattccg
     6481 aagccgaaaa aaacgttccg cgagaactac tgccacataa tcatcgaaag gccgatccgg
     6541 agaccgaagc ccaataggaa ttaaacgtgc caagccgttt gtatggtcct tcaaaaaacg
     6601 gttacgccct tcccggcttg aattgtgctc atcgatggat tttagcggta ggccgagagc
     6661 ggccagactc cggcgcacct gtttactatt ggttttatcg cccacgatca ccgagtcgat
     6721 cggatactca ctgcataatg aagtggcggt cgccatcaaa cgatctgtag atacgattac
     6781 tcgcttaaat acgatcgctt catcattgac cacagccatt ccgaattttg aatttcccgg
     6841 gtccactgca agtacggtgt aagaaattga aattcactcc ttagacacca aaaggtattt
     6901 cactttaaaa gcatccaccc ggtacagatc agccgcagct accagacata ccttgaagtt
     6961 tcccgtttta tggtctttaa tccgattaat gatctgagcc acctcggaga cggagacaag
     7021 atcccgcaga ttactaccat ccgtaattat ccccttctcg atcgccttgt tattggctaa
     7081 cagcaataac ccgatcaact ggtcccgcaa ttccgactcg gaagcggttg cggaaacctg
     7141 actttcctca atgatctccc catcccgaaa gatgagccga tcaggataga gttccagatt
     7201 gacggtggct ggttcaccac cggtcgagtt cttgtcgaca acaacccgta ataccgattt
     7261 tgttgttaaa gtcgccagtt gatcgcagat ctccgagatc cgctgcggca aactccgtaa
     7321 agccacatct ttctttcccg gaatccgcgc acctcgcttt agtgctaaat cattggcctc
     7381 atttaacatg ggttccacta cggaggatag aatcttgtct ttagggattc ctggttcaac
     7441 caccctggcg gtcatgatct ccccgacata gaagagaagc ggctgatcga gcattacctt
     7501 gttccgttcc tcaaggcttt tcatccatcc ctcaaggttt tggatctgct gattcagacg
     7561 ggcctcctgt aaagtcaaac tggagttctg attggttaga tcatgattca tcctggtcaa
     7621 actctttaaa cgatcctgca ccgcggcaag ctccgctttt cttgactgga ggttaagctc
     7681 tgcttgactg tattgcgctt gagtctttcg caacctatcc tcggccaaat tacgctgggc
     7741 agccacatcc tgcaatttta gatgtaactg gttatactgg ttttctttta aacccaactc
     7801 cctggtgagc gagaccactt tattgcgaat ggcattcaat tcgaagatgg cggtacgtac
     7861 atactccgac atcgccgtga ggattaagag ggtgcctccg gcgatcaaaa aaccagtcaa
     7921 aacagtaata atcatcgagg tatacttggg gcgcaatccg aataggctga ggcgtttttt
     7981 cccaaccctc ataccgaccc ggtcgcccaa gagcgcgatg aatccgccaa aggcgatcat
     8041 aatcataata atgagtatgc caaacatcat aaactccaac acataagttt gtcttcaacc
     8101 ccaattcggt agcaaattaa aaatggaacc gcatcggttt atttctgaat cgaggttcga
     8161 tatgacctcc ccaaaccggg gtttatagga tgatatactt aaatttttac ggttgtaaaa
     8221 acatatactc catacgcggc caagacaatg tcgccgagcc aagcaccaag gatcggattg
     8281 atagtaccgg cacggcccaa tgaggtgcca accatataaa gcagccacca agcgagaatg
     8341 aacaggaagc ataatccata tccggctgca ctggaacggc gctgcggccg caaagcgagc
     8401 ggcaccccca aaagggcaaa aaccagactg acgaagggaa tcgaaaactt ggtatgcaga
     8461 tagatcagga gtttagcctt ctcagggcct tgattgacca cgtttttgat ataccggctc
     8521 aattcagaaa tgctgcgttc ctccggagga agttgacgct gttcgatatc ggaaggtgtc
     8581 aagttcaacg ggtatctcac cttggcccga tccacggtga tcggatataa acttcccttc
     8641 cccaactcat tgatccggcc ccgctgaaaa aaccagctgt caccgttcca atccatcttc
     8701 tcggcctcaa tcgtccggag cagttgccca tgatcggctt ctacaataat caccttttgc
     8761 agttgctgca actgcggatc gtatttgtag gcataaatgt atttcttaag attaccgtcg
     8821 ttctcataca gatttagact gaagttatag atggtggacc gaagctcctg agacatcaat
     8881 ttggcctgca tatcgtcggc aatacggaac gataacggca ccacgtactc attgagcaat
     8941 acccccaaga tactgacaga aagcccgatg agcagcactg gagtagccac tttccgaaaa
     9001 ctcattcccc cggcccgcat ggcaatggtc tcactgttgc ccgtcagctt ccctaaaccc
     9061 aacaaagtgg cgagcagcac ggcgatggtc ccaccccaca tgatattctg ggggatcatc
     9121 agcaccagc
//
LOCUS       DASVMB010000409         7235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_51216,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000409 DASVMB010000000
VERSION     DASVMB010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51216"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="VF531_11640"
     CDS             complement(<1..1822)
                     /locus_tag="VF531_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7714658.1"
                     /translation="MKLAFSTLGCPDFSWPEIYSMAKDLGFDGVEIRGLGEEIFAVKA
                     QPFTESQLPQTQKKLAELHLEIPCLSSGCCLKFTGNAEHNCEEIMEYITLAAKLGTPY
                     IRILADLDPQPGGEVDDGVVLAALKRLIPMAEAKGITLLVETNGVYSNTDRLRRLLDS
                     AASDAVAALWDVHHPYRFGGETPGKTVQNLGAYIKYVHVKDSVVENGAIRYRMIGEGD
                     LPIDEIMLALRSINYEGYISLEWVKRWAADLSDAGIVFPHFANFMSRYTEKNTAKSHL
                     YDNMARTGKYVWEKDTLIDLTFPQVLDRMVDEFPNQYAFRYTTMDYTRTYSEFRDDVD
                     TFARSLIALGVKPGDHVAIWATNVPQWYITFWAATKIGAVLVTVNTAYKIHEAEYLLR
                     QSDTHTLVMIDGYKDSDYVGIIKELCPELASAEPGRPLHMKRLPFLRNIITVDSRQKG
                     CLTWDEAVAMAGSVPVEEVYRRALSINKHDVCNMQYTSGTTGFPKGVMLTHYNVVNNG
                     KCIGDCMDLSTADRMMIHVPMFHCFGMVLAMTASMTHGVTMSPIPYFSPKQSLACINK
                     EKITCFHGVPTMFIAMLEHEDFVKTDFSHMRTGIMAGSPCP"
     gene            complement(2579..3391)
                     /locus_tag="VF531_11645"
     CDS             complement(2579..3391)
                     /locus_tag="VF531_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HEX7714659.1"
                     /translation="MKLKTSFLSFACLLLLGSLLSPYSRACTIFSAQGATTHYFASNE
                     DWTMTDPAMHVVPSKDGNYGYIVFGWASLLPRYPQGGVNEHGLCLDWAALSPQSFKAN
                     PSRKQLNEDIIYKILRTCKNVDEVVRLVEQYNWAQFAEEHLLVADSQGDSCVIEWSDN
                     NYKFLRKDKPYQVITNFSLANPKIGWYPCQRFDTVDGYLKKAPTPEIGVETVRDLLNK
                     SHQEGQYPTVYSYIVDQKSLNVFVYYRHDYSKPFQYNFYEEVKKGEHRIRIY"
     gene            complement(3453..4157)
                     /locus_tag="VF531_11650"
     CDS             complement(3453..4157)
                     /locus_tag="VF531_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7714660.1"
                     /translation="MNDIAKFLKKLQQYHLPSVFNPWSDFDPELDISPAAPEIRSAQL
                     QQYLKLRLNKVRYLFIAEAVGYQGGRFSGIPLTSERILLGHHPRVRPEAVIGTQGIRT
                     SNPACFRLNPTEQAQGMAEPTATIVWGTLLGRGLDPESFVFWNIFPFHPYKPKSTGGF
                     LSNRTPSPAELECGAELFKEFRRLFPKARIFAVGRQADLTLQKMGLVATHLPHPANGH
                     ANQFRAAVGEDIKSSN"
     gene            complement(4634..4822)
                     /locus_tag="VF531_11655"
                     /pseudo
     CDS             complement(4634..4822)
                     /locus_tag="VF531_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(4876..5655)
                     /locus_tag="VF531_11660"
     CDS             complement(4876..5655)
                     /locus_tag="VF531_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7714661.1"
                     /translation="MNFKDKICVITGGANGIGKCLVREFSQKGAKVALVDLDRAAGEK
                     VLAEIRNGGGEGLFYCGDIAEKEVLWNFAAKVIATFGRVDYLINNACLSRRGIISGCD
                     YEDFNYVLKVGVTAPYMLAKLFRDHFAPGAAIVNISSTRSLMSQRDTESYTAAKGGIA
                     ALTHGLAISLTGKVRVNSVSPGWIDVRANNDPDYEKNHSLADKHQHPVGRVGRPLDIA
                     RAVMFLCDEENGFITGQNLTVDGGMTKLMIYSGDEGWDLTP"
     gene            5895..6797
                     /locus_tag="VF531_11665"
     CDS             5895..6797
                     /locus_tag="VF531_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714662.1"
                     /translation="MKEIALCCIMGAAGDHLALGAYLGPAGLNTLYQLADSALKVDDF
                     DKMFGQKCLMGSFEDRQMLDADDLSVIKELELKYRGHNQWPVFRSYEPGYYPWFLEAH
                     QCRFLTYIFQQGLDVCLRKRRNQVDFGDPSHNHFLIRRPHIDKNSINWTDEYQKIDPE
                     PTNYCSFKIVDELMLKRLRSLKIQPGLVLEADVFYFPTPIKEKGRPYFPLVSLLIEEE
                     QGIILGNEMFQDIKTEGYKCLDMLINQIQNIQKLPAKLLVSQDRSFFLYQDVCEQLGI
                     KLEQSDDLPVMEEVRKSMAEFLDQ"
BASE COUNT         1751 a         1866 c         1786 g         1832 t
ORIGIN      
        1 ccggacaggg actccccgcc ataatgccgg tcctcatatg ggagaagtcg gtctttacga
       61 aatcctcatg ctccaacatg gcgatgaaca tggtaggaac cccgtggaaa caggtgatct
      121 tctccttatt aatgcaggcg agcgattgtt ttggagaaaa gtaaggtatc ggagacatgg
      181 tgacgccatg ggtcatcgaa gcggtcatgg ccaggaccat tccgaaacag tgaaacatcg
      241 gcacatgaat catcatccgg tcggctgtgg aaaggtccat gcagtcgccg atgcatttgc
      301 cgttattcac cacattgtag tgagtgagca taacgccttt cgggaaaccg gtagttcccg
      361 aggtatactg catgttgcaa acatcatgct tattgatgga aagcgcgcgc cggtagacct
      421 cttcaacggg gacgctcccc gccatggcga cggcttcatc ccatgtcagg cagccttttt
      481 gtcttgagtc cacagtgata atgttgcgta ggaaaggtaa acgtttcata tgcagcggtc
      541 ttccaggctc ggcgcttgcc agttccggac atagctcttt gataatccct acataatcgg
      601 agtccttgta accatcgatc atcaccagcg tatgcgtatc ggattggcgg agcaaatatt
      661 cggcctcatg aatcttatag gcggtgttca ctgtgaccaa aactgcgccg atcttggtgg
      721 cggcccaaaa tgtgatgtac cactgaggga cattggtggc ccaaatggct acatgatccc
      781 cgggtttgac gcctaaagcg atcagggacc tggcaaaggt atcgacatca tcccggaact
      841 cggaataagt ccgggtgtaa tccatggtgg tatagcgaaa cgcatactgg ttggggaatt
      901 catcaaccat cctgtcaagg acctgcggaa atgtgaggtc gattaaagta tccttctccc
      961 aaacgtattt cccggttctg gccatattat cgtagagatg gctcttggcg gtatttttct
     1021 cagtgtaacg gctcatgaag ttcgcaaaat gcggaaagac aatgcccgca tcgctaagat
     1081 ccgcggccca gcgcttgacc cattccagtg agatatagcc ttcgtagttg atggatcgca
     1141 gcgccagcat gatctcgtca atgggcagat ctccctcccc gatcatgcgg taccggatcg
     1201 cgccattctc cactaccgaa tccttgacat ggacgtactt tatataggcg cccaggtttt
     1261 gtacggtctt gccgggtgtc tcgcccccga atctgtaggg atggtgaaca tcccacaggg
     1321 cggccaccgc atcgctggct gcagagtcga gcaatcttcg caggcggtcg gtgttggagt
     1381 atacaccgtt ggtctccacc aatagcgtga tacccttagc ctcggccatc ggaatcagcc
     1441 gcttgagggc tgccagaacc accccgtcat cgacctcgcc gcccggttgc ggatcaagat
     1501 cggccagaat tcggatataa ggagtgccga gctttgcggc gagcgtgata tattccatga
     1561 tctcttcgca gttatgctcg gcatttccgg taaacttcag gcaacacccg gaggagagac
     1621 aggggatctc cagatgtaac tcggcaagct tcttttgggt ctgcggtaac tggctttcgg
     1681 tgaaaggctg cgccttgacg gcaaagatct cttcaccgag tccacggatc tctacaccgt
     1741 caaaaccaag atccttggcc atcgagtaaa tctccggcca gctaaaatcg ggacaaccaa
     1801 gggtcgaaaa tgcaagtttc atcaaaatca tcctcctaat caatctttac cgcgggctta
     1861 caggtttaag tattaaaaat agaatttttg gcgacatagg ccgggattac ccttcccttt
     1921 gaatcagcga ctatatccgc ttcataaggg aagggtcgcg tcctatggga cgcggggtta
     1981 ggtataaaaa aagctttcgt ccctctgcat ttctgcaaga gacgaaagcc ttatgccctc
     2041 gtggtaccac tcttgttgat acatgcgtgt ttatacgcat gaaccctctt cttacgacat
     2101 cagtcatatg acgaatgccc tctcgtgata acggcgagaa acccgttcac acttacttgc
     2161 cgcatccgcc ctaacgacga ccgcggtttt cagctgactg ctcaagggtg agttcacaac
     2221 ctgcccggca cgctgtctct cagcaagcaa cagctctctg tagtaccacg tcgaggcgtg
     2281 ttttccctat caacgcattt gatattttta agtatattat caagttttac aaggtttgtc
     2341 aaggattatg acagacattt ttataacgaa ttgactacct ccgaaattgg cttcgcttta
     2401 atccactacc ccacaatccg gcaaggggat caatcactac ataaccattc cttggccctc
     2461 gtccgccgta tcaaaagcct tcaccgcacc cttggcattt ggtcctgcga tggtatttca
     2521 ttcattaagg gaatattccg gaaacggctt gccgaaactt ctctaccggg gacggaggct
     2581 agtaaatcct tattctatgc tccccttttt ttacttcttc gtaaaagttg tattgaaacg
     2641 gtttggaata atcatgtcgg taatagacaa agacattcaa tgatttctga tccacaatat
     2701 aagaataaac cgtcggatac tggccttcct gatgagactt gttcaataaa tccctgactg
     2761 tctccacacc tatctccggg gtcggcgcct ttttcaagta cccgtccacg gtatcaaaac
     2821 gctggcaggg ataccagccg atctttgggt ttgcaagact gaaattcgtg atgacttgat
     2881 agggtttatc tttacgcagg aatttgtaat tgttgtcgct ccattcaatc acacaggagt
     2941 ctccttggct atccgcgacc agtaaatgtt cctccgcaaa ctgcgcccaa ttgtactgtt
     3001 ccaccaggcg aacgacctca tccacattct tgcaggtcct cagtattttg tagattatat
     3061 cttcgtttaa ctgtttccgg cttggatttg ccttgaagga ttggggtgat aacgccgccc
     3121 aatccaggca taacccgtgt tcgttcaccc cgccttgggg ataccggggt aacaacgagg
     3181 cccacccgaa cacgatgtac ccgtaatttc cgtccttgga cggtacgaca tgcatcgcgg
     3241 gatcggtcat ggtccagtcc tcattgctgg caaaatagtg agtggttgct ccttgagcgc
     3301 taaagatggt gcaagcccgg gaatatggag aaagtaagga accaagaagt aataaacaag
     3361 cgaaactcaa gaaggatgtt ttcagtttca ttgaatttcc tcctttaatc tataccctat
     3421 cagttaccca aaaactttta actttaccct tttcaatttg aactttttat atcctcccct
     3481 actgctgcgc gaaattggtt ggcgtggccg ttggccgggt gcggcagatg ggtggcgacg
     3541 aggcccattt tttgcaaggt tagatcggcc tgacggccga cagcaaagat ccgggcctta
     3601 gggaaaagcc tgcgaaattc cttgaataac tcggcgccgc actcaagctc ggccgggctg
     3661 ggagtacggt tactcaaaaa gccgccggtg cttttcggtt tgtagggatg aaaggggaag
     3721 atgttccaaa atacaaagga ctccggatca aggccgcgcc cgagcagggt tccccagacg
     3781 atggtagccg tcggctcggc cattccctga gcctgttcgg tcgggttgag gcggaaacac
     3841 gcagggttgc tggtccggat cccttgggtg ccgatgaccg cctccggccg gacccgggga
     3901 tgatggccca gcaggatacg ctcggaggtg aggggaatgc cggagaaccg gccgccctgg
     3961 tagccgacgg cctcagcgat aaagaggtag cgaaccttat tcagccgcag tttgagatat
     4021 tgttgcaact gagcgctacg gatctccggc gctgcaggac tgatgtccaa ctccggatca
     4081 aagtcggacc aagggttgaa gacggagggg agatgatact gttgcaattt tttaagaaat
     4141 ttcgcgatgt cgttcattaa ttcgatctcc tcaaatccgg ggtttgggta tgtatcttcg
     4201 ttccccgtac tgtttcattt taccatgatt ttgggtgatc tatgatgaat agttgtatga
     4261 gagccagctt cgaggccgac gggggacggc gcgaacaaag ttcgcgagcc cactcccccg
     4321 cccccctagg gccaagggat tggaccagca atcccttgga acccaccggc ccaggggctt
     4381 ggcccccggg taccccctga tatcggcatt catccctgaa cgccgtcgga atgcttcgcg
     4441 attttacaga cgcctacctc catgtagccg tcttcttggg tgattctgcc tgtcgacggc
     4501 cgtgattcgt ccggaatcga ggccgcgtgg tcaacctccc tgttgaccat ttcggggtta
     4561 aaaattgtta tataaaaaaa cacgtcgatg agcgagcaag cccacgcatt tttcccctat
     4621 ctcttttcaa acttcacacc tcacccttca aacttccatt ctccctcata ctataaaacc
     4681 tcccctcccc gatcacaaga tgatccaaga ccctgatatt caagatctct ccggcctctt
     4741 gcaaccttct ggtaatcgca aaatccgccg ggctgggaaa cgggtcacga ctggggtggt
     4801 tcttgaaggc gatgatcgag gaaagcttga attctgataa aggttatgga agttagctct
     4861 attgtcgacg gggaatcaag gggtgaggtc ccacccctca tcaccgctat agatcatcaa
     4921 tttcgtcatg cccccgtcaa cggtgaggtt ctgaccggta atgaacccgt tctcctcatc
     4981 gcacaggaac atcacggccc gggcgatgtc gaggggcctg ccgacccggc cgaccggatg
     5041 ctgatgcttg tctgccaggc tgtgattctt ttcataatcc ggatcattat tggcccggac
     5101 gtcgatccag ccgggactga cgctattgac cctgaccttg ccggtaaggc tgatggcaag
     5161 cccgtgggtc agtgcagcga tgcctccctt ggctgctgta taactctcgg tatcccgctg
     5221 cgacatcagg ctacgggtgg aagagatatt aacgatggct gcgcccggtg cgaaatgatc
     5281 ccggaacaat ttggccagca tgtagggggc cgtaacaccg acctttaaga cgtaattgaa
     5341 gtcctcatag tcgcagccgg agatgatgcc tcgccggctc aggcaggcat tattaatcaa
     5401 atagtcgacc cggccgaagg tcgcgatgac tttagccgcg aaattccaca gcacttcctt
     5461 ttcggcgatg tcgccgcagt aaaagagtcc ttcaccgccg ccattccgaa tctcggccaa
     5521 gactttttcc ccggccgccc ggtccagatc gaccaacgcc accttggctc ctttttggga
     5581 aaactctctg acgaggcact tgccgatgcc attagctccg ccggtgatga cacagatttt
     5641 atctttgaaa ttcatggcgc acctcctctg atcattcata gttttaccgc ctatatttct
     5701 ttcattttac cataacctag tttcaagggc ccaatacccc ctataacaca gctattatag
     5761 gggacaagaa ggatttcttg gacaggattg aaattgtatt taagagaatc tcatttcgaa
     5821 aggatacttg atttcccaat gtttatcttc tacaattatc acatattatc gaagcatcct
     5881 atttttggag ttgattgaag gagattgctt tatgttgcat tatgggagcc gccggagatc
     5941 acttggcgct gggggcctat ctgggacccg cggggctgaa taccctctat caattggccg
     6001 attcggcgct taaagtggat gattttgata agatgttcgg ccaaaagtgt ttgatgggtt
     6061 cctttgagga tcggcaaatg ctggatgcag atgatctatc agttattaag gaattggagt
     6121 tgaaatatag agggcacaac cagtggccgg tctttcgcag ttatgaaccg ggttattacc
     6181 catggttttt agaagcgcat caatgccgtt tccttactta tatttttcaa cagggattgg
     6241 atgtttgttt acgcaaacgg cgtaaccaag ttgatttcgg tgatccatcg cataaccatt
     6301 tcctcattcg cagaccccat attgataaga actccattaa ctggacggat gaatatcaaa
     6361 agatcgatcc cgaaccaacc aactactgct cctttaagat tgtggatgag ctcatgttga
     6421 agaggctgcg ttctttaaaa attcagccgg gactggtcct ggaagccgac gttttttatt
     6481 tcccgacgcc gatcaaggag aagggtcggc cttatttccc tttagtttcc ctgctgatcg
     6541 aggaggaaca aggaattatt ctcggcaatg aaatgtttca agatataaag accgaaggat
     6601 ataaatgcct ggatatgctt atcaaccaga ttcagaacat tcaaaaatta cccgccaagc
     6661 tattggtatc gcaggacaga agcttcttcc tctatcaaga cgtctgtgag caactgggaa
     6721 ttaaactgga acaaagtgat gatttgccgg ttatggaaga agtgcgtaaa agcatggctg
     6781 aatttctcga tcaataagtc cacccgaagt gcgaccgcta tttttcatcc tcgaaaagtc
     6841 gaactccaaa gagttcgcag tggcgcccgg actccttcca tggagtccgg aaacgaagga
     6901 ctagccagat agctaaaaaa cagttatgct cctaaaaatt taaacgccgt cggagtgctt
     6961 cgcgatataa tagacgacta cagcgcgtac acatatgcac gcgtccatgt agccgtcttc
     7021 ttgggtaatt cctgcttgcc gacggtgcga cttgttaaca agccgcgccg tcggccaatt
     7081 aaaccacccg agaaggccac cgtaacggag gcggtggcta gttaaatcaa gtattcctac
     7141 tccaaccgcg gatgggagga gatatagcag gggtccgggg agctcccgga cataggcctc
     7201 atgatattac aattctcccc tctcccaacg ttctt
//
LOCUS       DASVMB010000410         5371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_275258,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000410 DASVMB010000000
VERSION     DASVMB010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275258"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VF531_11670"
     CDS             complement(<1..788)
                     /locus_tag="VF531_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX7714663.1"
                     /translation="MHPLLQSLSVGQVTLANRLVMPPMATSKANPDGRVSRALLDYYD
                     EKSSGGYVPLIIVEHSYIKPEGKAGQGQLSVADDAVITGLRELAEVLHKNGSKGIIQM
                     NHAGSAAKEEVICTKPVGPSAVAHPRGGAIPRELTPEEIGELVTAFAAAASRAKAAGF
                     DGVEIHSAHGYLLNQFYSPLTNRRTDEYGGELLNRIRIHLHVIAAIRAAVGEDFLVLL
                     RLGASDYTAGGTTVEDSQIAAGEFERAGVDILDISGGFCSYSVPG"
     gene            complement(960..1988)
                     /locus_tag="VF531_11675"
     CDS             complement(960..1988)
                     /locus_tag="VF531_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7714664.1"
                     /translation="MKVLFIGGTGVISEGVSRVAVERGVDLYLLNRGQRNLLFPNGAK
                     QIQGDIREPQSISEILRNHHFDVVVDWVAYNPEHVRTDIDLFRDRADQYIYISSASAY
                     QKPVMDYLITESTPLANPYWQYSRDKIAGEELLMNEYRKTGFPVTIIRPSLTYGLSMI
                     PASVNSWQKPWSLIDRIRKGKKIIIPGDGTSLWVITHNTDFAKGIIGLFGNRRAFGQA
                     FQITSDEVLTWNQIYEEIGRAAGAKINPVHITSDFLAEVIPDARGNLIGDKVQSVVFD
                     NSKIRRYVPGFFATIPFAEGIRKTVQWFEAHPEFSVVDEAWDHLMDRIIEEYEAALTT
                     VKARIIST"
     gene            complement(2106..3977)
                     /gene="abc-f"
                     /locus_tag="VF531_11680"
     CDS             complement(2106..3977)
                     /gene="abc-f"
                     /locus_tag="VF531_11680"
                     /inference="COORDINATES: protein motif:HMM:NF000355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HEX7714665.1"
                     /translation="MIDIALNGVEKYYGANQILKGVTLEIQEGERVALLGLNGTGKST
                     IFKILAGTEGCDKGERILRKGISIGFLEQIPLYAEGYTVREVLYSAFQDLWAVRDEML
                     RLEKLMTDNPTEEVLKKYGRLQELFESCAGYSIEERINRVSQGMKIDQGWYDQEFNSL
                     SGGEKTRVKLAQLMLESPKVLLLDEPTNHLDLGTIEWLENFLKDYPGTIFAISHDRYF
                     LDKLVTRVLELVNGQIDNYQGNFTEYLREKEERYQNQLKRYEAEQKKIEQLEAAAKRM
                     HEWAKVADNPAMHRQAFSIEKRIERMEKTERPPAVKGMNNRFTEKDFSGEMVLVTEQV
                     TKAYNQKTILDGCELTVRKGERVAVFGDNGSGKSTLLKILVGELSPDSGTVRIGASIR
                     YGYLPQSVSLPDPAATILEMVRRELRESEYQVRRTLAKFNFRNEDVLKTVGNLSGGEK
                     TRLKLCLLMQQELNLLLLDEPTNHLDIASRLWLEEALTDFQGAMIFVSHDRYFVNRFA
                     TRIVELSQGLISEFAGDYEFYKLKKAEASAQKITQKPEKQKSPQNLPKGLSRAEQPDL
                     RKLEADISAWEAQMALLDERIRLEENNYERLMVLAKEREAIESKINDLYEQWFEFHD"
     gene            complement(4475..5302)
                     /locus_tag="VF531_11685"
     CDS             complement(4475..5302)
                     /locus_tag="VF531_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714666.1"
                     /translation="MPKSLSMLLKNSFAWLISIIVLIPFAVMLVNSFKTKEESYSMTL
                     KLPAVFQFHNYMTVIQQGNITSSFLNSFMYAAICVVVVVLTCSMAAFVISRNETRLNR
                     FVYYLIIMGIILPVNYVALMKVMQVTLLNNTRIGISLLYAAMNIPISLLITTSFINTI
                     PKELDEAAIIDGCGPWSLFSHIIFPLLKPVMVTVGLLGFMSAWNDFMMPLYYLNNSSK
                     WPMSLTVYGFFGASMQAQRQWNLVCADIVLTSAPVIIVYLLGQKYIVSGVTAGAIKG"
BASE COUNT         1408 a         1422 c         1132 g         1409 t
ORIGIN      
        1 ccggggacac tatagctaca gaaacctccc gagatatcca ggatatccac cccggctctt
       61 tcaaattctc cggctgcaat ctgactgtcc tccaccgtcg ttccgcctgc agtataatcc
      121 gaagccccca gacgcaatag aaccaaaaaa tcttctccga ctgccgcccg gatcgcagca
      181 ataacatgga gatgaatccg gatccggttg agaagttccc cgccgtattc gtcggtccgg
      241 cggttagtga ggggcgagta gaattgattg agtaaataac catgggccga atggatctcc
      301 acgccgtcaa atccagccgc cttggcccgg ctggcagcgg ctgcgaaggc ggtaaccaac
      361 tctccaattt cctccggtgt cagttcgcgg gggatggctc cgccacgggg atgggccact
      421 gccgaaggac caacaggttt ggtgcagata acttcttcct tcgctgcgct gcccgcgtgg
      481 ttcatttgaa taatcccctt ggagccgttt ttgtgaagga cctcggccaa ctcccgtaga
      541 cccgtaataa ccgcgtcatc cgccacggaa agttgaccct gaccggcttt cccttccggc
      601 ttgatgtagc tatgttctac aatgatcagc ggaacataac ctcctgagga cttttcgtca
      661 taataatcca aaagcgcccg gctcacccgg ccgtcgggat tcgccttgga cgtagccatg
      721 ggtggcatca ccaggcggtt ggccagggtc acctgaccga ctgataaaga ttgtaataat
      781 gggtgcaagc aaatcgcctc caggttaaga attgtgaaaa gtgaagtgcg cccacgcgcg
      841 ctcgaagtgt gaaaaaaacc aaatatcatt ccaccatatg ttttaattga ttattagcct
      901 ttccggattg gccaaaaaat tcatgatcga cactatcacc gttagaaata ttataaaatt
      961 caagtactga taattctagc tttgacggtg gtgagtgccg cctcatattc ctcaataatc
     1021 cggtccatca gatgatccca agcctcgtcg acgactgaaa actcgggatg ggcttcaaac
     1081 cattgaaccg tcttacgaat cccctcggca aagggtatcg tggcgaaaaa tccggggaca
     1141 tatcgtctaa tcttgctgtt gtcaaaaacc acgctctgca ctttgtctcc gataagattg
     1201 ccccgggcgt cggggatcac ctccgctaaa aagtcggagg taatatggac cgggttgatt
     1261 ttggcgccag cagcccttcc gatctcttcg taaatttgat tccaggtaag tacctcgtcg
     1321 gaagtgatct gaaaagcctg accaaaggca cgcctgtttc caaaaagtcc gataatgcct
     1381 ttggcgaaat cggtgttatg ggtaatcacc cacaaggagg tcccgtcacc ggggatgatg
     1441 atctttttgc ccttccggat ccggtctatc agcgaccacg gtttttgcca actgttgacg
     1501 ctggccggga tcatactcag gccatacgtg agcgaaggcc tgataatcgt caccgggaaa
     1561 cccgttttcc ggtattcgtt catcaataac tcctcaccgg cgattttatc gcgggagtac
     1621 tgccaatagg ggttggccag gggagtcgac tcggtaatca ggtaatccat caccggcttt
     1681 tgataggccg atgccgaact gatataaata tattggtcgg cccgatcccg gaataggtca
     1741 atgtcggttc gtacgtgctc cggattatag gcaacccagt caacgaccac gtcaaaatgg
     1801 tgattccgaa gtatttcgga gatactttgc ggttccctga tgtctccctg aatctgtttg
     1861 gcaccgttcg gaaaaagcag attacgctgc ccgcggttga gtaaatacag atcgactccc
     1921 cgttcaaccg ccacccggga taccccttca ctgatgacac cggtaccgcc aataaataac
     1981 accttcatgg gtcagcctct tttcgaaacg gattttccgg attaaatgcc ggtccagatt
     2041 ttatgctagg tatttcgacc cgaataacag atttacttct ttatgggtaa atttttgaca
     2101 atcaattaat cgtggaattc aaaccactgt tcatatagat cattgatttt actctcgatc
     2161 gcttcccgtt ccttggcaag caccatcaat cgttcgtaat tgttttcttc gagccggatc
     2221 cgctcatcca agagcgccat ctgagcttcc catgctgaaa tatcggcctc tagctttcgt
     2281 aagtccggtt gttccgctcg ggaaagaccc tttggcaaat tttgcggcga tttctgcttt
     2341 tccggtttct gggttatctt ttgagccgag gcctcggcct ttttaagctt ataaaattca
     2401 taatcaccgg caaattcgct gatcaggcct tgactcaatt caacgatccg ggtcgcaaac
     2461 cggttcacaa aataccgatc atgggaaaca aagatcatcg ccccttgaaa atccgtcaac
     2521 gcttcttcca gccacaggcg ggaggcaata tccaggtgat tggtgggctc atccaacaaa
     2581 agtaaattaa gctcctgttg cataagcaaa cacaatttca accgggtttt ttcccctccc
     2641 gacaaattcc ccaccgtttt caggacatcc tcattgcgaa agttaaactt tgccaaagta
     2701 cgccggacct gatattcgct ttcccgcagt tccctccgaa ccatctccag gatggtggct
     2761 gccggatccg gtaaactcac tgactgcggc aagtagccat accgaatact tgcgccaatc
     2821 ctgaccgttc cggaatccgg ggagagctca ccgacgagaa tctttaataa ggtcgatttc
     2881 cccgatccat tatcgccaaa aacggccaca cgctctcctt tgcggaccgt cagctcacaa
     2941 ccgtccaaga ttgttttttg gttataggcc ttggttacct gttcggtcac aagcaccatt
     3001 tcgccggaaa aatccttttc ggtgaagcgg ttattcatcc ccttaacagc aggcggcctt
     3061 tccgtctttt ccatccgctc aatccgtttt tcgatgctga atgcctgccg gtgcatggcg
     3121 gggttatccg ccaccttcgc ccactcatgc atccgtttgg cagccgcctc aagctgttca
     3181 atcttttttt gttccgcctc ataacgtttc agctggtttt gatatctttc ctctttctcc
     3241 cggagatact cggtgaagtt tccttggtaa ttatcaattt gaccgttcac cagctccagc
     3301 acccgggtga cgagcttatc caaaaagtag cggtcgtgtg aaatggcaaa aatagtcccc
     3361 gggtaatcct ttaaaaaatt ctccaaccac tcaatggtac ccaggtcaag atggtttgtg
     3421 ggttcatcca gcaaaagaac ctttggggac tccagcatca attgggccag tttcacccgg
     3481 gtcttctctc cgccgctcaa ggaattaaac tcctggtcat accaaccctg atcaatcttc
     3541 atcccctgcg aaacccgatt aattcgctcc tcaatcgaat aaccggcgca cgactcaaat
     3601 aattcctgaa gtctaccgta ttttttgaga acctcttcgg ttggattatc ggtcatcagc
     3661 ttttccaaac gcagcatctc atcacgaacc gcccacaaat cttgaaacgc cgaatatagc
     3721 acttcccgga ctgtataccc ctcagcatac agggggatct gttccaaaaa gccaatggat
     3781 atccccttgc ggagtatcct ttctcccttg tcgcagccct cggttccggc caaaatcttg
     3841 aagattgtgg attttccggt tccatttaac cccaataagg cgacccgttc cccctcctga
     3901 atctccaggg taacaccttt taagatttgg ttggctccat agtacttctc aacaccattc
     3961 aaagcaatat caatcatggc tctccttccc acccggaaat tcgtagatat gtgcgtttac
     4021 gcgcataaaa aatctgcctg agtagtagcc cgggcagttt ccggatgatg gttcgccacg
     4081 ctcccgagac cctctctctg agcctgccaa acatcatcgt acatgttcca ccggttccaa
     4141 accggtttat gtacataaaa caaaacccgg gcaagtaatc tcccgggtgt aatcgctttt
     4201 aaagtaatcg tttttcggaa aataaattac ttgctcagtt tacgtattca attttggata
     4261 gacttccccc tttgaaaact tgaaaatcac aaattgccga gacataatgg gaaagccggt
     4321 tcaaaatcat actcactgag cactgagagt tcatcatttc acctcctttt aacgatatca
     4381 atttaccatg aaacccaact tattgcaatg ttaattttat ctttaatcat ggccccaata
     4441 gcatacgtgc atttattcat taagtatcaa aaactcaacc cttgattgca ccagcggtaa
     4501 cccctgagac aatgtatttc tgccccaata aataaacgat gataaccggc gcactggtta
     4561 aaacaatatc ggcgcagacc aggttccact gtctttgggc ctgcatactg gcgccaaaaa
     4621 aaccatacac tgtcagtgac atcggccatt tcgacgagtt gttcagataa tataatggca
     4681 tcataaaatc gttccaagcc gacataaaac ccaacaatcc aaccgtaacc atcaccggtt
     4741 ttaatagcgg gaaaataata tgggaaaaca aactccatgg tccgcatccg tcaatgatcg
     4801 ccgcttcatc cagctcctta gggattgtgt taataaaact ggtagtaatg agaagactga
     4861 ttggaatatt catagccgca tataaaagac taatgccaat ccgggtatta ttaagcaaag
     4921 tcacttgcat cactttcatc agggcaacat aattgacggg taagatgatt cccatgataa
     4981 taaggtaata aacaaatctg tttaacctgg tctcattgcg ggagattacg aatgccgcca
     5041 tggaacaagt taataccacc accaccacac atattgcagc gtacataaaa ctattcagaa
     5101 acgaagaagt aatattgcct tgttgaataa ctgtcatata attatgaaac tgaaaaacag
     5161 ccggtagttt tagggtcatt gaatatgatt cttctttagt tttgaaggag ttcaccaaca
     5221 ttacggcaaa gggtatcaat acaataatac tgatcaacca ggcaaagctg tttttaagca
     5281 gcatgctgag tgatttaggc attagtattc cacttccttt ttatttagag ccttaataag
     5341 ggaataacag atcccgcaaa cagcgacgaa t
//
LOCUS       DASVMB010000411         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_126851,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000411 DASVMB010000000
VERSION     DASVMB010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126851"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VF531_11690"
     CDS             <1..783
                     /locus_tag="VF531_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX7714667.1"
                     /translation="IGLEIFLLPNDIIDGGVIGISIITSYLTKIPLGILITVLTLPFL
                     WVGYKQIGKSFVISTLFAVVSLSFWVTILHPVPGLTKDVLLATVFGGVILGIGVGLII
                     RTGGSLDGTEIVAVILDKRTGFSVGEIVMFFNLFILSSAGFVFGWNKAMYSLIAYFIA
                     FKMIDVVIRGLDEEKAAWIVSKKGDAIREAIMYRLGRGATVLEATGGYTNTPKTLIYS
                     VVTRLEVAKLKQIVHEIDETAFVTINDVNDVMGGKHHKRAIH"
     gene            complement(948..1934)
                     /locus_tag="VF531_11695"
     CDS             complement(948..1934)
                     /locus_tag="VF531_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2278 family protein"
                     /protein_id="HEX7714668.1"
                     /translation="MPLDSYSVLKCRPIKGLAGSNQSPHYQIHCLDGNATHYRIAVNI
                     HSQTNPSDLLYYADDNFQHSITSQLTELKFGLTPLKSQPGGAALDYIRGNLFDYHAMQ
                     ILPGDIPGADNDLNEKLDKYVEWALKMADAEIYAFGNAWGPENKPDQYFGFTPGRGIH
                     DIHMNQGNSGRWADDNGPWQDGGIFFHFPAVRKWVAIFLAFQSQTFHSVDGTGQPIQF
                     QDAVSDGTVRIVAAQVNVTGPGNQTVTLANTGSHAIDLIDWAILDGLKRKQILKGMIG
                     AGEFRTIPINGKEAQFSGQGGIISLLNQNGMKIAGVAYTKEDCRKSGCTIVF"
     gene            2270..2728
                     /locus_tag="VF531_11700"
     CDS             2270..2728
                     /locus_tag="VF531_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714669.1"
                     /translation="MIRDKMVFGGMIGFLANVAMNFFQYPLWKLKVIEHPLAHYAGSM
                     FMDVETLHHTLLGSFISFVANSSYSAFLGIFFVYTVYFTGGHAFIVKGLLFGAFVWLF
                     SFGGLRSLPMVQLREYISSQALYYLFIHLVFGLALGLLINKFGEPALRKE"
     gene            complement(2754..>3268)
                     /locus_tag="VF531_11705"
     CDS             complement(2754..>3268)
                     /locus_tag="VF531_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7714670.1"
                     /translation="LPDYREDNELGLGYSKLSRIISGYTPPNPDSFKVMDNGAPEEHP
                     ELLLIGNFSVADLGFEVFEGNGGHLHGEMVFVCRKLGLIFTGDILVNIAGFSRERAEF
                     NSLAPYLMRSVNLDSQKATVIRKQVTSLIDTIQTVNQTPCIICGGHGPLSMLANGKLE
                     NFPNTEIISW"
BASE COUNT          786 a          742 c          827 g          913 t
ORIGIN      
        1 atcggtttgg agatttttct tttgcccaat gatatcatcg acggcggagt cattggcatc
       61 tccattatca cgagctattt gaccaagatt ccgctgggaa tattgattac ggttttgacc
      121 ctgccctttc tttgggtggg ttataaacag attggcaagt ctttcgtcat atcgaccctg
      181 tttgcagtag tttcattatc tttttgggtc accatcctgc accccgttcc cggcttgacc
      241 aaggatgttt tgctggctac ggtctttggc ggcgtgatat tgggaatcgg ggtggggctg
      301 atcatccgga ccggcggatc gctcgatggg acagagattg tcgcggtgat cctggacaag
      361 cggaccggct tctcggtggg cgagattgta atgtttttca accttttcat cctgagcagc
      421 gccggttttg tcttcggttg gaacaaggca atgtattccc tgatcgccta ctttatcgct
      481 tttaaaatga ttgatgtggt gatccggggg cttgatgagg agaaggccgc ctggatcgta
      541 tcgaaaaaag gggatgcgat tcgggaggcg atcatgtacc gcctgggccg tggagccacc
      601 gtactggagg cgaccggcgg gtataccaat acccctaaga ccttgatcta ctccgtagtc
      661 acccgtttgg aagtggccaa actgaaacag atcgtccacg aaatcgatga aaccgctttt
      721 gtcacgatta atgatgtcaa tgacgtaatg ggcggtaaac atcacaagag agcgattcat
      781 taagagcaac ttgccaaccg cttccccagc caccttcaac gccctgccgg ttgggagtgt
      841 cgcaaaacta ctatttaagc agtaaaccct tccctttgga cgcggggtta ggatgatttt
      901 aaacatcgag cccagccgta aacggctggg cgattgattt acttggacta aaatacaatc
      961 gtgcaaccgc tcttccggca atcttccttg gtgtaagcca ccccggcgat cttcatcccg
     1021 ttttggttga gtaaagaaat gataccccct tggccggaga attgtgcctc ctttccgtta
     1081 atcggtatgg tacgaaattc tccggcgcca atcatgcctt ttaaaatttg tttgcgcttg
     1141 agaccgtcca aaatcgccca atctataagg tcaatggcat gactgccggt attggccaag
     1201 gtcacggttt ggttgcccgg gccggttaca ttcacttgcg cggcgacaat gcgaacagta
     1261 ccatcggaga ctgcatcttg gaattgaatg ggttgcccgg ttccatcgac gctatggaat
     1321 gtttgagact gaaacgccaa aaatatggct acccatttgc ggacggcagg gaagtggaaa
     1381 aagatgcctc catcctgcca gggaccgtta tcatcagccc atctgccgga gttcccctgg
     1441 ttcatatgaa tatcgtgaat gccccgtccg ggcgtgaaac caaaatattg atcgggtttg
     1501 ttctccgggc cccaggcatt gccgaaggca tagatttcgg catccgccat tttgagggcc
     1561 cactctacat atttatcgag tttttcgttg aggtcattat ctgctcccgg tatatcaccg
     1621 ggtagaattt gcatcgcatg ataatcaaac agattgccgc gaatataatc cagcgccgct
     1681 ccgccgggtt gactctttaa gggagtcagg ccaaatttga gttcggtgag ttgtgatgta
     1741 atactatgtt gaaaattgtc atcggcgtag tacagcagat ccgaaggatt cgtttgggag
     1801 tgaatattga cggcaatgcg atagtgagtg gcgtttccgt caagacaatg gatttgataa
     1861 tggggacttt ggtttgaacc agccaggccc ttaatgggtc ggcattttaa aaccgagtac
     1921 gagtccaaag gcatgattct catcctccca taatttggta tatgtaaatt atatgatatt
     1981 caaaaaggat tatcaatgaa tgagctcaaa gtatgtcagc catttcaaat tcacagttca
     2041 gaagacgcgt gcatttgatt ggtttaaagt tggcagagtg agcttgaatt ggagatagtt
     2101 gctgccgcat aaaatagcgg tacgggagga aagttatctc aagatcaagg aaggaggaaa
     2161 acaataaatc gcgggcagca cgggctcgcg tacacgcact tgaacatagg aaattcgtta
     2221 tcctccagtt aatgccgttt tagtcggttc tcaaaatgag gtgtaatcca tgattcgcga
     2281 caaaatggtc tttggcggga tgatcggttt tttggccaat gtcgccatga acttttttca
     2341 atatccgtta tggaagctta aggtgattga gcatcctctg gctcattatg cgggcagcat
     2401 gtttatggac gttgaaacgc tacatcatac cttgcttggt tcgttcatca gttttgtcgc
     2461 caacagttct tatagcgctt ttttgggaat tttctttgtt tatacagtat atttcaccgg
     2521 aggacacgct ttcatcgtga aaggtctact ttttggcgcg tttgtatggt tattttcttt
     2581 tggaggcctt cgctcacttc ctatggttca actccgggaa tatatctcca gccaagcatt
     2641 gtattatctc ttcatccatc tggtattcgg tctggctttg gggttattga tcaataaatt
     2701 tggagaaccg gcgttacgta aagaatgatt ttcgcgtgag cgcgtaacca tctttaccat
     2761 gaaatgattt cagtgttggg gaaattctca agctttccgt tggccaacat cgaaaggggc
     2821 ccatgaccgc cacagatgat gcagggtgtt tgattgacgg tttggatggt gtcgatcagg
     2881 ctggtgactt gttttcgtat cacggttgcc ttttgggaat caagattgac gctgcgcatc
     2941 aggtatggag ccagagaatt gaactccgcc cgttcccggg aaaagccggc gatattgacc
     3001 aggatatccc cggtaaatat caggccaagc ttccggcata caaagaccat ttctccgtgg
     3061 agatggccgc cgttaccctc gaacacttca aaaccaagat ccgccacaga aaaattcccg
     3121 ataagtaaaa gttccggatg ctcttccggc gcgccattgt ccatcacctt gaaagaatcg
     3181 gggttcggag gcgtataccc ggaaataatc cggctcagtt tgctgtatcc caaaccgagt
     3241 tcattgtctt cacggtaatc cggaagcc
//
LOCUS       DASVMB010000412         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_566847,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000412 DASVMB010000000
VERSION     DASVMB010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566847"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VF531_11710"
     CDS             complement(<1..688)
                     /locus_tag="VF531_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7714671.1"
                     /translation="MKGLRFVNMWIDSHAHLNDTAFEPDYREVLQQAIAGGVDAVIVP
                     GFDLESSRKAVAMAQVEPRIWAAVGFQPEELESWTPGALEEMADLLNHPKVVAVGEIG
                     LDYHYGSSNREEQLAVFRAQLDLARRFRKPVIIHSREAAQDTLTLLQEARAAIPETDG
                     FTGVMHCFSGSWETAQQYLQLGLEISFAGPVTFPKALNLQRVAQLIPPERLLVETDSP
                     YLSPTPFRGQR"
     gene            complement(666..2627)
                     /gene="metG"
                     /locus_tag="VF531_11715"
     CDS             complement(666..2627)
                     /gene="metG"
                     /locus_tag="VF531_11715"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7714672.1"
                     /translation="MGKFYVTTPIYYPSDNLHIGHAYTTVIADALARYHRQIGDDVWF
                     LTGTDEHGQKIQRKAEAAGVTPQAYVDRIVTGIRELWSKFKISNDDFIRTTEPRHEKA
                     VQMIFERLYNQGDIYKSEYEGWYCTPCEAFWLERQVKEGKCPDCGREVELVKEESYFF
                     RASKYAERLIRYIEDHPDFIQPVSRKNEMVNNFLKPGLEDLCVSRTTFNWGIKVPFDP
                     KHVVYVWLDALSNYITALGYPAETEQFKKFWPADLHLVGKEIVRFHTIIWPIILMALD
                     LPLPKQVFGHGWLVLDGGKMSKSKGNVVDPLVLIDKYGLDAIRYYLLREIPFGSDGSY
                     TEDALILRINTDLANDLGNLLHRTLSMLEKFNQGVIPSPGTYQELDRQLMNQAQESVS
                     ELKRAMESLEVSAGLIAIFKLVSRANKYIDEVAPWALAKQPGERERLSTVLYTMAETL
                     RLVATLLVPFLVETPDKIWDQLGLNGSPAALDYQDAVRWGWLKPNTSTRKGSPIFPRI
                     ETEAPGKQAPLAPGVSATPERPVTQVPATKTPAAPAPEQITIDEFARIDLRVAKVLAA
                     EKVEGSDKLLKLKVKVLGSERQIVAGIAQHYQPEALVGKEIVVVANLKPAKLRGLLSE
                     GMLLAASNDQGQVIVVRPEAEAGEGSKVR"
     gene            3421..3672
                     /locus_tag="VF531_11720"
     CDS             3421..3672
                     /locus_tag="VF531_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7714673.1"
                     /translation="MMMKSTGIVRKVDELGRVVIPIELRRTLQIDEKDALEIYVDGEK
                     IILKKYEPACIFCGNAEDIRNFKEKNICKACFESMKNAG"
     gene            complement(3867..>4281)
                     /locus_tag="VF531_11725"
     CDS             complement(3867..>4281)
                     /locus_tag="VF531_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7714674.1"
                     /translation="RGMADYRGTLVFYEAPHRLLNTLKNLYLVLGDRPAVLARELTKV
                     HEEFIRGPLSRLVTELEGNPERLRGEFTIVVSGSDVSAESGRDDEPELEAMIASLAAG
                     KFRSLREGIKEVARESGRSAKEIYQLYLKLQGNRE"
BASE COUNT         1068 a         1154 c          966 g         1093 t
ORIGIN      
        1 tcctctgacc acggaatgga gtcggcgata gatagggact gtctgtctcc accaacagac
       61 gctccggggg aatcagctgt gccacccgtt gcaaattgag ggctttcggg aaagtcaccg
      121 gccctgcgaa agaaatctcc agacctaact gcaaatattg ctgagccgtt tcccaactac
      181 cgctaaaaca gtgcatcact cccgtgaatc catccgtttc gggaatggct gccctggcct
      241 cctgcaataa cgtcaatgtg tcctgtgcgg cctcccggct gtgaatgatc accggtttcc
      301 ggaaacggcg cgccagatcc agttgcgccc ggaagactgc cagttgttct tccctgttgg
      361 aactcccgta atggtaatca aggccgatct cgccgacagc aaccaccttg ggatggttaa
      421 ggagatcggc catttcttca agagcccccg gggtccaact ctcaagttct tcaggttgaa
      481 accccactgc cgcccagatg cgcggctcaa cctgcgccat ggctacagcc tttctggaag
      541 attccagatc gaaaccagga acgattaccg cgtcgacccc gccggcaatg gcctgttgga
      601 gtacctcccg gtaatcgggt tcaaacgcgg tatcgttgag atgggcatga ctgtcaatcc
      661 acatgttaac gaaccttaga cccttcaccc gcttcggcct cgggacggac cacgatcacc
      721 tggccctgat cattcgaggc tgcaagcagc attccttcgg agagtaaccc cctgagtttg
      781 gcgggtttca aattggcaac caccacgatc tccttaccca ccagcgcttc cggctgataa
      841 tgttgagcga ttccggccac aatctggcgc tcactcccca acaccttaac cttgagtttc
      901 aatagcttat ccgaaccctc tactttttcg gcagccaata ccttggcgac ccgcaggtca
      961 atcctggcga attcatcgat ggtaatctgt tccggtgccg gcgcggccgg agttttcgtg
     1021 gcaggaacct gagtcaccgg ccgttcggga gtggcggaaa ctcccggggc cagaggcgcc
     1081 tgcttgcccg gagcttcggt ctcgatccgc gggaagatgg gcgagccctt ccgggtcgat
     1141 gtattcggct ttaaccaacc ccacctaaca gcatcctgat aatcgagggc tgccggtgat
     1201 ccgttaagtc ccaattgatc ccagatctta tccggggttt ccaccaaaaa cggaaccagc
     1261 aacgttgcaa caaggcgcaa ggtttcagcc atcgtatata aaacggttga taaccgttcc
     1321 cgctctccgg gctgtttggc aagagcccat ggagctacct catcgatata tttattggcc
     1381 ctgctcacca atttgaagat ggcaatcaaa ccggcgctca cttccaggct ctccatggcc
     1441 cgtttgagtt cactcacact ctcttgggct tgattcatca gctgccggtc cagttcctga
     1501 taagttcccg ggctgggaat caccccttga ttaaatttct ccaacatcga cagtgtccgg
     1561 tggagcaaat tacccaagtc gttggcgaga tcggtattaa tcctcaggat caatgcatcc
     1621 tcggtataag aaccgtccga tccgaaggga atctccctga gcaggtaata acgaatggca
     1681 tccagaccgt acttgtcgat caggaccaat ggatcaacca cgttcccctt ggatttggac
     1741 atcttaccgc catccaacac caaccagccg tggccgaaaa cctgctttgg caaaggcaaa
     1801 tcgagggcca tcagaataat cggccaaatg atcgtgtgaa acctgacgat ctccttgccc
     1861 accagatgga ggtctgccgg ccaaaacttc ttaaattgct cggtttctgc ggggtacccc
     1921 agggcggtaa tatagttgga cagcgcatca agccagacgt agaccacgtg cttgggatca
     1981 aagggaacct taatccccca attgaatgtg gtccgggata cacagagatc ctcgagtccc
     2041 ggtttgagga aattattcac catctcgttc ttgcgggaga ccggctgaat aaaatcggga
     2101 tgatcttcga tatagcgaat cagtctctcg gcatactttg aagcccggaa aaaataactc
     2161 tcctctttaa ccagctccac ctcacggccg caatcggggc acttgccctc tttgacctgg
     2221 cgttccagcc aaaaggcttc acacggtgta caataccagc cttcgtactc actcttgtaa
     2281 atatcgcctt ggttgtacag gcgctcgaaa atcatttgaa ccgccttttc atgtcggggt
     2341 tcggtggttc ggataaaatc gtcgttacta atcttaaatt ttgaccataa ctcgcggatc
     2401 ccggtcacga tccggtcaac atacgcttga ggtgtaactc cggcagcttc cgccttgcgt
     2461 tgaatctttt gaccatgttc atccgtacct gtcaaaaacc agacgtcatc acctatctgg
     2521 cgatgataac gggctaacgc atcggcaatc accgttgtat aagcgtgccc gatatgcaga
     2581 ttgtcactgg gataatagat cggtgttgta acatagaact tacccatttg ccatacctcc
     2641 ttgcctaaaa tagatattct ttccggtttt accaaaaaat aaaacccctc atcccaaagt
     2701 acggggacga gaggttaacc ttcgtgttac caccccagtt cactgaatac agcttgcggt
     2761 caaaccacgc aggccacatc agactccaag gttcctaacg cgaaccgaat cccacggcca
     2821 atcttacaac aaccgaaatc aacaattcat tcccgatatc ggctgcctcg gacggcgact
     2881 ccggaaccat ctttcccgaa ctttccaatc aggtttccac cagccccgat tctctttacg
     2941 gatcataacg ggaactcttt ccatcattgt ctttacggga tatattcaaa ataatttttc
     3001 aaccagggtt cggtaactac tgtatcctga tgacccaaag aagttaccga gttaaggtta
     3061 aatataccgg ttcatccttc gtttgtcaag tagtaaccgc ctatgtaaat atttagtgaa
     3121 cttcttggat taatttgact ttgtatgtaa atgcaggtat aataaatctt ggtttgcaaa
     3181 tgtcgaattt taacggaaat gatttacgaa gcaatccaat ctaaaataca ttcttcatgt
     3241 ttctgccttt acctggaact cgccggcgaa acctgcttta tacctgagtt attctcttgt
     3301 gagacattat tcttttgtaa gttttggtga taatttgctt ggatttattt gactttacat
     3361 ctatttttag gtataatata accatcgtta atgtcgaatt ttaacgaaag gagtgatgac
     3421 atcatgatga aatcaacagg aattgtaagg aaagttgacg agcttggacg ggtggtcatc
     3481 ccgatcgaat tacgccgtac gctccagatt gacgagaaag atgcccttga gatttatgtt
     3541 gatggcgaaa agatcatttt gaaaaagtat gaacccgcct gcatcttctg tggtaatgcg
     3601 gaagatattc ggaatttcaa ggaaaaaaat atctgtaaag cttgttttga atccatgaaa
     3661 aacgccggct gattgtactt attcattccg gtttcccaaa tcaggcaata agattcgttc
     3721 taatacgagg ttgtttcttt aaaaaaggga caacctcttt ttttatctcg tcatgaaaca
     3781 tcgggaaagg caacagggac taggcaatag gcaacagatt tagtcacaca taaaaatgcc
     3841 tgttgcctgt tacctgttgc cagtccctat tccctgttgc cttgaagttt caagtacagc
     3901 tggtaaatct ccttggcact ccgtcccgac tcccgggcta cttctttgat accttcccgc
     3961 agactccgaa acttacctgc tgctaaggag gcaatcatcg cttcaagttc cggttcatcg
     4021 tctctgccgg attcggcaga gacgtcagat ccggagacga cgatggtgaa ttcccctctt
     4081 agccgttccg ggttaccttc caactccgtt accagccggc tgaggggtcc gcgaataaac
     4141 tcctcgtgta ccttggtgag ttcccgggct aaaaccgcgg gccgatctcc caacacgaga
     4201 taaagatttt tcaaggtatt caggagccgg tgaggggctt cataaaaaac caaagttccc
     4261 cggtaatctg ccattcctcg g
//
LOCUS       DASVMB010000413         5347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_275308,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000413 DASVMB010000000
VERSION     DASVMB010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275308"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF531_11730"
     CDS             <1..348
                     /locus_tag="VF531_11730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7714675.1"
                     /translation="YSSSGIFIVPAIYAFSHSLYTIGAFNFLVGAIFSGVNLALFNAL
                     LEVTPEVRKTTYIAYYNTLITISGVIAPIAGVSLLGFMNFQWAFIICAVLRILGSLGF
                     LVISRLEERERRF"
     gene            complement(355..2421)
                     /locus_tag="VF531_11735"
     CDS             complement(355..2421)
                     /locus_tag="VF531_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX7714676.1"
                     /translation="MKPFHSFTLKYIFPGLFLAALLIIPGSVTGAVAPADPGSNAATS
                     LYYQGDYQASLDEWQKLIDSRPEKARRNLVQLLREAERYDEALNQLDELLKTAPQDPD
                     YRQTRLELSFLAGHSEQVVADCKLDQASAAELFWLGMALLDLGKPDEAGQIFKKSLAK
                     EGYNPAALYQLGGMSLARRDFGSARDYFRAALAQDSNWTPSYFPLAKAYIGLANFTAA
                     NNLLTNAGMVFPEDPEIAATLQQLLADHPELAAEPPQETKARREVASPKQVNPILEGR
                     ESIPTIRIGLAEGISQLFLKTSPEYTLTTMDLKTIAAGSENTVLQFKANPEGITVLNE
                     AGTVLYQTAQPLILTYENPSATTILFDMAYGQGSFWAGRADRTYRGRIELIPMSQSAT
                     APTITVVNQLNVEEYLYAVVPSEISSSWPRAAQEAQAIAARTYAFANLGGFAGRGFDL
                     MATVASQVYNGVGAETPAARAAVDATRGQILTYNGKPIAAFFHGNSGGFTGSSADLWN
                     LDLSYLRPQADLLLPATVAGGRMDPADLWNWLLSRPLTYSSQPKYSAASSYRWSFWIS
                     RVQLERRLNQGDKLGQIRSLTISGRGDNGMVKQVLITGTGGSYRLKGDKIRSAMGGLR
                     SNLFVVEAKLGKDGLPEYFIFSGAGWGHGIGMDQSGAAGMAAAGYSSTDILKHYYPGT
                     QLVKKY"
     gene            2666..4021
                     /locus_tag="VF531_11740"
     CDS             2666..4021
                     /locus_tag="VF531_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7714677.1"
                     /translation="MMLKRLSSLLILVLLVFGYSVTGFAKERVSVLAFDGKVDEQYKQ
                     AAVNNLNKILLNFGKYDVVERAEVEKIIQEQNFQMTDLVDPTTAVQVGKILGVSIAFI
                     GNIDQLTYSWNKTSYQAVAKITIKVIDVQTAKILNIIEGRGTSSGVDRSATLHEALDA
                     CFTDQILKDFRVKIAPFSSIIKVEGENLYFLNGKDVGLKKGMRFQVLRPEANDFGDDA
                     ATDESVETEFATADDSFKRAIGLVEVAEVIADKSRAIIIWNSEPIRRGDVLAEVENPN
                     RGLISFGLRTTNSTVEDSSGVKTTDPYAKVYEGRFLSELPYSYSSGIIFNYTPLNKAI
                     GDFNLGWEYTKEFPLIRGSLYLTGMGGLGFGWSSQTTNLTGYAATAGSLYATAGGGLK
                     LYLGHEDGVRVEFGAVGQFGPVLNRWTENTTENDMTDWVDYPKADLSGFGLRLMVDIP
                     L"
     gene            4082..4675
                     /locus_tag="VF531_11745"
     CDS             4082..4675
                     /locus_tag="VF531_11745"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7714678.1"
                     /translation="MHCLMIYAHPGEKSLSGALGQAFLEGLRAKGHSAEVIDLYREQF
                     DPALAADELYTREGFQISTPVKAYQEQLQKADIIALAFPTWWQGTPAILKGFLDRVLT
                     PGVAFEYQGRKPKGLFTDKVAVILNCYDASAFEVRSVNGDRAFKVLQKDVFGTCGIRK
                     IFRVSVFSAGTSSERYRRKMLESARKLGMEISTVAMR"
     gene            complement(4920..>5347)
                     /locus_tag="VF531_11750"
     CDS             complement(4920..>5347)
                     /locus_tag="VF531_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX7714679.1"
                     /translation="MFEIIQEQLRRDGIEWYRIDGQTPPPERLRLVHQFNNGGAPIFL
                     ISLKAGGTGLNLTGADTVIHYDPWWNPAAEDQATDRAYRIGQTNTVQVFKIVTHGTIE
                     EKIFVLKQQKKELVEVVIQPGADFLTRMTLAEIQGLFAD"
BASE COUNT         1218 a         1340 c         1492 g         1297 t
ORIGIN      
        1 tactcctcca gcgggatctt cattgtgccg gccatttacg ccttctccca cagcttgtat
       61 acgattgggg ctttcaactt tttggtcgga gcaatctttt ccggagtaaa tctggcgctc
      121 tttaacgcgc tcttggaagt aacacccgag gttcgcaaaa ccacatacat tgcctactat
      181 aacactttaa tcacgatttc cggtgttatt gccccgattg ccggggtcag tctgcttggg
      241 ttcatgaatt ttcaatgggc ctttattatc tgtgcagtct tgaggatctt gggtagtctc
      301 ggatttttgg tcattagccg gttggaggaa cgggaaaggc gtttttaaat ctcttcaata
      361 ttttttaacc agttgagttc cgggataata gtgtttcaaa atatccgtac tggaataccc
      421 cgccgcggcc ataccggccg cgccgctttg gtccattccg atgccatgcc cccagccggc
      481 gccgctaaag atgaagtatt ccggcaggcc atccttgccc agtttcgctt cgacaacgaa
      541 cagattgctg cgcaacccgc ccatggcgga acggatctta tcacccttca gccgatagct
      601 gccgcccgtt ccggtaatca acacctgttt caccatgccg ttatcacccc ggcccgaaat
      661 ggtcagggaa cgaatctggc cgagtttgtc cccttggttt aaacgtcttt ccagctggac
      721 ccgggagatc cagaaggacc agcgatatga gcttgcagcg gaatatttcg gttgcgaact
      781 gtaggtaagc ggacggctta acagccagtt ccaaagatcg gcaggatcca tccggcctcc
      841 ggcaactgtt gccgggagca gcagatcggc ttgaggcctg agataagaga gatctagatt
      901 ccacagatcg gcgctgctgc cggtgaaacc gccgctgttg ccatgaaaga aggcggcaat
      961 gggtttaccg ttataggtca ggatttggcc ccgggtagcg tctaccgctg cgcgagccgc
     1021 aggcgtttcg gcgccgactc cgttatagac ctgggaggct actgtagcca taagatcaaa
     1081 tccacgaccc gcgaaaccgc ccagattcgc gaaagcataa gtcctggcgg caattgcctg
     1141 ggcctcctgt gccgctctcg gccaggaaga ggagatctcc gaaggcacca ccgcatataa
     1201 atactcctcc acattcaatt ggttaaccac tgtaatcgtt ggcgctgtcg cactttgtga
     1261 catggggatt agctcgattc gcccccggta agtgcgatcg gctcgaccgg cccagaaaga
     1321 accctggccg taagccatat caaacagaat ggtggtggcg gatgggtttt cataagtaag
     1381 aatcagcggt tgggctgttt ggtagagcac tgtaccggct tcattaagca cagtaatccc
     1441 ctctgggttg gccttgaact gcaagaccgt gttctccgaa ccggcggcaa tggtcttcaa
     1501 gtccatggtt gtcaaggtat attcgggact ggtcttgagg aatagctggg agatcccttc
     1561 ggccaggccg atccggatcg tgggaatgct ttctctccct tccagaatgg ggttcacttg
     1621 cttgggtgaa gccacttccc gccgggcttt ggtttcctgg ggcggttctg cggccagctc
     1681 cggatgatcg gccaataatt gttggagtgt tgcggcgatc tccggatctt cgggaaagac
     1741 cattccggca ttggtcaaca gattattggc cgccgtgaag ttggccagtc cgatataggc
     1801 cttggccaat ggaaaatagg atggagtcca gttggagtct tgggccagcg cggcccggaa
     1861 ataatcgcgg gctgatccaa agtcccggcg tgccagactc atcccgccca attggtatag
     1921 ggcggccggg ttataaccct ccttggccaa agatttttta aagatttgcc cggcttcatc
     1981 cggcttaccc agatcgagca gggccattcc caaccaaaaa agttcggctg cagaggcttg
     2041 atcaagcttg cagtcggcta caacttgctc ggaatggcct gcgaggaagg atagttcaag
     2101 ccgggtctgc cgataatccg gatcctgggg ggcggttttg agtaattcat ccaattgatt
     2161 caaggcttca tcgtaacgtt cggcctcacg taacagttgt accaggttac gccgggcttt
     2221 ttccggccgg gaatcgatga gtttctgcca ctcgtcgaga cttgcttgat agtcaccttg
     2281 atagtaaagg gaggtggctg cgttactgcc tgggtcggca ggtgcaaccg cccctgtaac
     2341 cgatccgggg atgatgagca gagctgccag gaagagacct ggaaaaatat acttcagagt
     2401 gaatgaatga aaaggtttca tagtgccctc acatagttta ttaattgtat tgtaatttga
     2461 aacccgcacc gcgtcaactg gagaacaacc aggaacttgc ggggcggagt tgacgaaact
     2521 tggatttggg tcgattaccc tcagcctgaa aacggaagtt ttggaaaccg ttgacctcag
     2581 ttaacgcagt ttggaaggag acgcccgaat cgtgtcgaat aaatgagaat aatagcgttt
     2641 tgaatgattt tttggagggg ttgaaatgat gcttaaacga ttgagtagcc tgttgattct
     2701 ggtattgctg gtgtttggtt attcggtgac cgggttcgcc aaggaacgtg tcagcgtcct
     2761 ggcctttgac ggaaaggtgg atgagcagta caagcaggct gcggtaaata acctcaataa
     2821 gatattgtta aatttcggca agtatgacgt ggtggaacgg gccgaagtgg aaaaaattat
     2881 tcaggaacag aatttccaaa tgaccgattt ggttgatccg accactgcag ttcaggtcgg
     2941 gaagatcctg ggagtcagca tcgcttttat cggaaatatc gatcagttga catactcctg
     3001 gaataaaaca tcctatcagg cggtggccaa gattacgatt aaagtcatcg atgttcagac
     3061 tgccaagatt ctaaacatca ttgagggcag ggggacaagc tccggtgtcg atcgatccgc
     3121 taccctccat gaagccttgg acgcctgttt caccgaccaa atcctcaagg atttccgggt
     3181 gaagatcgcg cccttcagtt caatcatcaa ggtggagggt gaaaatcttt atttcctcaa
     3241 cgggaaggat gtagggctca aaaaaggaat gcgttttcag gtactccgtc ccgaagcgaa
     3301 cgatttcggg gatgacgccg ctacggatga atcggttgaa acggagtttg ccactgcgga
     3361 cgactccttc aaacgtgcca tcggtctggt ggaagtggcc gaggtcatcg ccgacaaatc
     3421 ccgggccatc attatttgga attcggaacc gatccgcagg ggcgatgtcc tggcggaagt
     3481 ggagaatccg aaccggggcc tgatcagttt cggattgcgg accaccaata gtaccgttga
     3541 agattcgagc ggcgtcaaga caaccgatcc atacgccaag gtttacgagg gccgtttcct
     3601 gagtgaactg ccatactcct attccagcgg catcatcttt aattatacgc cgttaaacaa
     3661 agcaattggc gattttaacc tgggttggga gtacaccaag gagttcccgc tgatacgggg
     3721 aagcctttat ctcaccggaa tgggcgggct gggtttcggc tggtcctcac agaccactaa
     3781 cctaaccggc tacgccgcca ctgccggaag tctttacgca accgcaggcg gaggcctcaa
     3841 gctttatctg ggccatgagg acggagtgcg agtggagttc ggtgccgtcg gccagttcgg
     3901 cccggttttg aaccgttgga ccgagaacac tacggagaac gatatgacgg attgggtaga
     3961 ttatcccaaa gccgatctct ccggtttcgg gctccgtctg atggtggata ttccgctata
     4021 agttaggatt taaacagtgt ttggcttata acgaagcatc gatatcggga ggtcttttct
     4081 catgcattgt ttgatgattt acgcccatcc cggggagaaa agtctcagcg gcgccttagg
     4141 tcaggctttt ttggagggac tgcgcgccaa gggacattcg gctgaggtca tcgatctcta
     4201 ccgggaacag tttgatccgg cgttggcagc cgacgaactc tacaccaggg aaggctttca
     4261 aatctccacg ccggtcaagg cctatcagga gcagctgcag aaagcggaca tcattgccct
     4321 ggcctttccg acctggtggc aaggaacccc cgccatactt aaggggtttc tcgatcgggt
     4381 gctgactccg ggtgtggcct ttgaatatca gggccggaaa cccaagggcc ttttcacgga
     4441 caaggtggcg gtgatcctca actgctacga tgcgtccgct tttgaggtcc gcagcgtcaa
     4501 cggcgatcgt gcctttaagg tgctgcaaaa agatgtcttc ggaacctgtg ggattcggaa
     4561 gatcttccgg gtctcggtgt tctcggccgg tacctcctcg gagcgctacc gccgtaaaat
     4621 gttggaaagc gccagaaaac tgggaatgga gattagcacc gtggcgatgc gttaacataa
     4681 taccagggga gcgggctcat cgccggcgcc ctgaagcctc agtcatactt gggttaggaa
     4741 acggggtccg gattggcccc gtttcatatt tatcggtaag gaagcatccg ccagcggttg
     4801 atggagatct accgggaaat gatccatgat tgcggtacgg aggctcgtat cagcaaccga
     4861 gagtcgtgat gataaccaat ctgaacggtt gccacacaat accgggatct aaaaacggct
     4921 taatccgcaa acaacccctg gatttccgcc agcgtcatcc gagttaagaa atccgccccg
     4981 ggttggatga ctacttcgac caattctttt ttctgctgtt tcaggacgaa gatcttctcc
     5041 tcaatggtgc cgtgagtcac gattttaaat acttgaaccg tattggtctg gccaattcgg
     5101 tatgccctgt ccgtggcctg atcttcggcg gccggattcc accaggggtc gtaatgaatt
     5161 acggtgtcgg cgccggtgag gtttaatccg gttccgcctg ctttcagtga gatcaaaaat
     5221 atcggggcgc cgccgttgtt gaattgatgt accagccgta gccgttccgg gggtggagtt
     5281 tggccgtcga tccggtacca ttcaatgccg tcccggcgca attgttcctg aatgatctca
     5341 aacatcg
//
LOCUS       DASVMB010000414         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_347435,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000414 DASVMB010000000
VERSION     DASVMB010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347435"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1018)
                     /locus_tag="VF531_11755"
     CDS             complement(137..1018)
                     /locus_tag="VF531_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714680.1"
                     /translation="MEHCLRPLKTVFRSEKNTHKAVNTVLFIVLSIGAIVMIAPLIWM
                     LSTSVKSRMDVFALPPVWIPKTFNWSKYQEIWVKGPLLSGIVNSLIVTITVTVVGTFT
                     SSLAAFAFAKLRLPHKKMIFLMLLATMMIPYPVVMIPQFVMFSRFGWVDTLKPLIVPG
                     LFGNVMMIFFLRQYIMSGVPDELIEAAKMDGCPIFKIYYKIVLPLIRPAIVAQFILWF
                     MAIWNDYLAPTIYLSSPEKRTLQIVIASFNSTYAIQTDYPLIMAASVVSVLPMLLVFM
                     IFQKQIVESVALTGVKG"
     gene            complement(1040..1954)
                     /locus_tag="VF531_11760"
     CDS             complement(1040..1954)
                     /locus_tag="VF531_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017812882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714681.1"
                     /translation="MEAPADRVASNPKNKSSLYQYENRIAFLFILAPIIQFFVFTLVP
                     CLYSLYGAFTDWNGLGQMNWIGFGNFIEMVGEVRFWKAMYNTIFMMLGIPIGLILALL
                     LAMALNRDVIGTKVFRVIYYVPVISSLAAVSILWQWAYNGDYGLINQFLAIFHIKGPN
                     WLQNEYTIKPAIIIMAIWKGLGYSMLLYLAALQNIPRIYLEAVKLDGASAFQTFKHII
                     IPLVGPVTFFIVVTSIINGSQMFIEPNIMTIDGGPMYSAGTTVYYLWQKAFNNYQMGY
                     ASAVAWVLGVFIFIVTLIQFKYNDRHDY"
     gene            complement(2117..3421)
                     /locus_tag="VF531_11765"
     CDS             complement(2117..3421)
                     /locus_tag="VF531_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714682.1"
                     /translation="MALVLGGFFTIGIAKEQTEISLMFKGQPQEQEAYQKVIKSFEAA
                     NPNINVKLILTSNDEYTAKIQAAIAARKMPDVFYINPGDIKAWVSAGLLLDITKYVEH
                     SNIVKIDNMWALGVNKYRYDGKKVGRGALYGLPKDIGPFALGYNKTMFEKAGLPLPDK
                     DKPYTFDEFINVCKQLTKDTDGDGKVDQWGTGFNVNWVLQAFVWSNGADWIDDTKTKV
                     TINDPKFIKALQYFADTQNVYHVTPSIQEAQTLDTYQRWMRGQLAFFPVGPWDLAVYD
                     GLKFDYDLMPYPAGSTGKPATWIGSLGFCAAKNTKHPKEAADLIMYLSGDPKGQQELV
                     DLKVQIPNIKDKAREWAQDSMIKPSNKQEFLDIVNDYGRAFPTEYTYNAEWYNDFFSN
                     IQPVLDGKITAAEYCKQEQPRMQRLLDKAITQEKAARKQAKK"
     gene            complement(3573..>4074)
                     /locus_tag="VF531_11770"
     CDS             complement(3573..>4074)
                     /locus_tag="VF531_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7714683.1"
                     /translation="KTGVLNIFIEKLGQDSIMFLGDAALFKHVYVWSGVWQEMGWASI
                     IYIAALTNVDPALYEAASVDGASKFQKMWHIDLPQIMPIAVTLLILGIGNLMSVGFEK
                     AYLMQNNLNLESSEIISTYVYKIGLLGAQFSYSTAVGLFNSIVNCVLLLATNSISRRF
                     TNTSIW"
BASE COUNT         1147 a          967 c          781 g         1179 t
ORIGIN      
        1 ctcgatgaat gatccgaaaa ggtggggatg aacttcaccg atggtaaaat ttttgtctgc
       61 gaaatacttg atttttttca tttgtgttca tgttctccct gatccgatta attttatcac
      121 attatttcct gaaatgtcat cccttgactc ccgtcaaggc caccgattct acgatctgct
      181 tttggaagat cataaaaacc agcaacattg ggagcaccga caccaccgat gccgccatga
      241 ttagcggata atcggtttga atcgcatagg tcgaattaaa actggcgatc acaatttgca
      301 aggtccgctt ttccggggaa ctcaaataaa tggtcggcgc caaatagtca ttccaaattg
      361 ccataaacca caaaataaac tgggccacaa tggccggccg gattaatggc aagacgattt
      421 tgtagtatat tttaaaaatg ggacatccgt ccattttggc cgcttcgatc agttcatccg
      481 gcactccgct cataatatat tgccgcaaaa agaagatcat catcacattg ccgaatagac
      541 ccggcacgat taatggtttt aaagtatcta cccagccgaa ccgggaaaac atgacaaact
      601 gcggtatcat tacgaccgga tagggtatca tcatcgttgc caataacatc aagaatatca
      661 tttttttatg cggtaatctc aatttggcaa atgcaaaagc agccaagctt gaagtaaatg
      721 tccctaccac agtaaccgtt atcgtaacga tcaggctatt gacaattccg gacagtaaag
      781 gccctttgac ccatatctcc tgatatttgc tccaattgaa tgttttcggt atccagaccg
      841 gaggcaaggc aaatacatcc attcttgatt ttacagaggt cgacagcatc catattaaag
      901 gggcaatcat gacgatcgct ccgatcgaca gaacgataaa aagaacggta ttgacagctt
      961 tatgagtatt tttttcagag cggaataccg ttttaagcgg ccgtaagcag tgttccatct
     1021 ccggcaactc ctttccgttt taataatcgt ggcggtcgtt atatttaaac tgaatcagag
     1081 tcactataaa tatgaagacc cccaaaaccc atgcaacggc agatgcgtat cccatttggt
     1141 aattattaaa ggctttttgc cacagataat aaaccgtcgt tcccgcgcta tacatcggcc
     1201 cgccatcgat agtcatgata ttgggttcga taaacatctg cgatccattg atgattgaag
     1261 taaccacgat aaagaacgtt accggtccga ccaaaggaat gatgatatgt ttaaaggttt
     1321 gaaatgcgct cgcgccgtca agttttacag cttccagata aatgcgggga atgttctgga
     1381 gtgccgccag atacaacagc atggaatagc ctaatccctt ccatatcgcc ataataatga
     1441 tagccggttt gatggtatat tcattttgta gccaattggg tccctttata tggaatatcg
     1501 ccaagaattg attaatcaat ccgtaatccc cgttgtaagc ccattgccac aatattgaaa
     1561 ccgcagccaa cgatgaaata accggaacat agtagatcac tctaaatact tttgtcccga
     1621 ttacatccct gtttagcgcc atggccaata gcagcgccaa aatcaaacca atgggtatgc
     1681 ccagcatcat aaagatcgta ttatacatgg ctttccaaaa ccgcacttcg ccaaccattt
     1741 cgataaaatt accgaagccg atccaattca tctgacccaa tccgttccaa tcggtaaaag
     1801 cgccatatag ggagtagaga caaggtacca atgtaaaaac gaaaaattga atgattggcg
     1861 cgagtatgaa caaaaacgca atccgatttt catattggta gagactggat ttgtttttcg
     1921 gattggatgc cactcgatct gccggtgcct ccatattgcc cggctgtaac cttaatcgca
     1981 ttttcccatc tcctgccgtt cttagaaacg gaggatatcc ccgactatgt ttcgggtcct
     2041 cctttgatat ggctttttac aaatactaac ccgacaaagc ctgcaaaacc ggctttgtcg
     2101 ggttgatatg agtgatttac tttttagctt gcttccgggc tgctttttcc tgagtgattg
     2161 ctttatccaa aagcctctgc atcctgggtt gttcttgctt gcaatattcc gcggcggtta
     2221 ttttcccatc cagaaccggt tgaatgttgc taaagaaatc attataccat tccgcgttgt
     2281 atgtatattc cgttggaaac gccctgccgt aatcgtttac gatgtcaaga aattcctgtt
     2341 tgttggaggg cttgatcata ctatcttgag cccattccct cgctttatct ttgatattgg
     2401 gaatttgtac tttcagatcg accaattctt gttggccttt gggatcgccg gataaataca
     2461 taatcaagtc cgccgcttct ttgggatgtt tggtgttttt ggcggcgcag aagcccaacg
     2521 atcctatcca ggtagccggt tttccggttg aacccgccgg atagggcatg agatcataat
     2581 caaactttaa tccatcatag acagccaaat cccacggccc gacagggaaa aaggcgagct
     2641 gtccccgcat ccagcgctgg tatgtgtcaa gagtttgagc ttcctggatt gaaggtgtta
     2701 catgatagac attttgcgta tccgcaaaat attgtaatgc cttaatgaat ttaggatcat
     2761 tgatcgttac cttggttttc gtatcatcga tccaatccgc gccattactc cacacgaaag
     2821 cttgcaaaac ccaattgaca ttgaacccgg ttccccattg atctactttc ccatctccat
     2881 cggtatcttt cgtaagttgt ttgcatacat tgataaattc atcaaatgta tagggtttgt
     2941 ctttatcagg caacggaagt cctgcttttt caaacatagt cttgttataa cccaaagcaa
     3001 acggtccgat atccttaggc aatccgtaca atgcgccccg tcccactttt ttgccgtcat
     3061 accggtattt atttacccct aatgcccaca tgttatctat ctttacaata ttcgaatgtt
     3121 ccacatattt cgtaatatcc agcaataatc ctgcactaac ccaggcctta atgtctcccg
     3181 gattgatata gaatacatcc ggcattttcc tggcagctat ggccgcttgt atttttgcgg
     3241 tatattcatc attggatgtc aaaatcagtt ttacattgat attaggatta gcggcttcaa
     3301 agcttttgat taccttctga tacgcttctt gctcttgggg ctgtccctta aacatcaggc
     3361 tgatttcagt ttgctccttg gctatcccga ttgtaaaaaa gcctcccaac accaatgcca
     3421 tacttaaccc aataaccaca gtcctgaaaa taaatttcgt accgaatact tttcccatac
     3481 caatacctcc ttttttatat taagccatcc ttgcggataa cttgacccta tataaaaaaa
     3541 tgcagctcgg tcaagtgata gaatcatctt ctttaccaga tactcgtatt tgtgaatctc
     3601 ctcgagatag aattggtagc cagtaaaagc acacagttaa caatcgaatt aaagagtcct
     3661 acagctgtgg aataactaaa ttgcgccccc aacaaaccga ttttatagac ataagtcgag
     3721 atgatttcgg aagattccag gtttaaattg ttctgcatca gataggcctt ttcaaaaccc
     3781 accgacatca agttgccaat tcctaaaatc aatagtgtaa cggcgatggg catgatctgc
     3841 ggcaagtcaa tgtgccacat tttctgaaat ttactggctc catctacgga cgctgcctca
     3901 tataaagccg gatccacatt ggtaagcgcc gctatatata tgatggatgc ccatcccatc
     3961 tcctgccaga cacccgacca gacatacaca tgtttaaata atgccgcatc tcccaagaac
     4021 atgatcgagt cctgacccaa tttctctatg aatatgttta aaaccccggt tttc
//
LOCUS       DASVMB010000415         7859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_420522,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000415 DASVMB010000000
VERSION     DASVMB010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420522"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            572..763
                     /locus_tag="VF531_11775"
     CDS             572..763
                     /locus_tag="VF531_11775"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX7714684.1"
                     /translation="MGDVPRRVQSRHLYSSGEPEPSPGDIFLTQRLKEAGRIMCIELL
                     DHVITGERDYYSLREKGLM"
     gene            1561..3831
                     /locus_tag="VF531_11780"
     CDS             1561..3831
                     /locus_tag="VF531_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HEX7714685.1"
                     /translation="MFSPQKVVLTIVLVWVTVFSTNVFAETICFDGPMGSACPMEALD
                     RGLIAMKVETGVFLSWRFLKNEVTGYSTTGLTGVNFNIYRDGVKIATVTDSTNYLDDS
                     GTGSSKYYVRAIIKDMEFNKSATVTPWANPYYDLPLNIPAAVTLPDGVTPTYSANDIS
                     VGDVDGDGKYEYFVKWDPSNSKDVIQKGYTGIVYLDCYKQDGTLLYRIDMGVNIRAGA
                     HYTQFMVYDFDNDGKSELIMKTAPGTKTVRYRPDGSVASQRYITLPPWDIAAGYKNTD
                     DYRSTDTSYYEYLVQVFKDWRSHPEVVNGHWPNTLEACFGIANQYTYPLSDADARQLV
                     DYFMDVYAPSRSTRNALRSFTGYIFKGPEYLTVFNGATGSEMQTIPYKPGRDDDGLMW
                     GDYAMSRIEPCNRVERMQACVAYLDGQRPYAVFGRGYYTRTTLVAYGWDGTRLREYWY
                     VDSGYPMMNNPFNSGPHGMDGRNAAFATITTQGAHTLTAADVDNDGCDEIIAGAATID
                     HNGHLLYSSFAPLNGVNTRLGHGDSIHVADIIPDRPGLEIFMCHEGGASAPYGVSMRD
                     AGTGETLWGTPKSTDNGRCMIGDVYPSYRGIESWSMALRAGDAIGTEISTVMPGTNAN
                     IKWASDLTTQFIDGSITATPTIKGWSVDNTPTTILTCNDTYTNNYTKGNPCLVADILG
                     DWREELLVRKVDSSAIRIYSNSEITTHKLFTLMQDRQYRTDVARQNDGYNQPAYTSFY
                     LAFDMDWQTLYRTLLP"
     gene            4150..4755
                     /locus_tag="VF531_11785"
     CDS             4150..4755
                     /locus_tag="VF531_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7714686.1"
                     /translation="MKISKRQAQKDQTRKQLIEVAFEQFARDGLVAARTSDIAEAAGV
                     SHGTVFAHFATRDDLLIAVIEEFGSRVAGRIHELAGSGGSVREVLQAHLNGLIEVEPF
                     YSRLVAERLLLPSEAQSALVSIQSAIAIHLYQAVEGGIIAGTIRNIPLHLLFNTWISL
                     IHYYLSNADLFAPGKSVLRRYGPELLEHYLGLISLPGGNHE"
     gene            4789..5514
                     /locus_tag="VF531_11790"
     CDS             4789..5514
                     /locus_tag="VF531_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714687.1"
                     /translation="MTKTVENLLRNSGLPGPRGNLRLMYSFAHQATRDEINECLAFYN
                     DKLANSPEEFVVMCGIVGYCVLNQSHLQDTLTVIRGYASHQSWRIREAVAMGIQEIAS
                     ANMEAILDNLENWLTGDELEKRAVVAALCEPKLLLGEKAVNLRIVNILAKLALEFAEI
                     DGKLSENQNALRKALGYGWSVVIVAIPDEGKAAFEGMAGNSNKHIQWIVKENLKKHRL
                     QVMDKKWVEKMMAVCAEKGDISI"
     gene            5511..5711
                     /locus_tag="VF531_11795"
     CDS             5511..5711
                     /locus_tag="VF531_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714688.1"
                     /translation="MTGQVKSKTKLKARTCYGHLGGKLGQLLFERLVELEWFTLEEGR
                     ATVYQVTEKGRQELGKLGVPIE"
     gene            5793..6035
                     /locus_tag="VF531_11800"
     CDS             5793..6035
                     /locus_tag="VF531_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7714689.1"
                     /translation="MKTCIACGMPMVKKEEFAMGDMSKDYCVFCARPDGSMQSYEEKL
                     AGMTEFIVKTQGFDKNAALEAARQMMAKLPAWQGRI"
     gene            6405..7790
                     /locus_tag="VF531_11805"
     CDS             6405..7790
                     /locus_tag="VF531_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HEX7714690.1"
                     /translation="MLAKGKGTNIKTVRPFGLADKLSYMMGDFGCNMSFVLVQSYFML
                     FYVTVMGISPVHFGIIILITKIWDGINDPLIGALTDYLQPKGGKDKFRPWILWTSAPI
                     TIATILMFAYLPGIPYWAKITQCVVTYLIWDICYTCVNVPYGSLQSVITDDAVERAEL
                     SRFRTLGAFIVQLAVLAALPKFIFVDKNPVGGRFFLAAIILSVMGFIAFQILYKFSIE
                     RIKVIAPEQEVKYNYFRTFAAFFKNRPMMALTISSISTLLLVGSTAVLNQYVFITYYQ
                     NPGLLSIGSVISMVPVLVAMIFVKMAVKKFGKKAISSWPILGAALCYLYLLVVPVPNP
                     YVWFGLQGLAALLSGFYTMLTWAMVADCIDYQEWQTGRREEGSIYATYSLFRKFAQGF
                     GASLVAFLLAFTGYNAGLQADQLPGVAENIKFLAALLPLVGNVIIFLAMQLIYNLDNK
                     GLAELKKNLGR"
BASE COUNT         2127 a         1681 c         1999 g         2052 t
ORIGIN      
        1 cgacccctag gacctacccg cccgcaacct caccccttta ttcccctctc ccaacgtccc
       61 ttatgggaga ggggaaaatt gttatgtcac agggcctatg tctgggagct ccccggaccc
      121 ctgctatatc tcctcacatc cgtgttcgag taggagtttt tgatttatta gccgggcact
      181 tccatgtacc cggcttctgg gtgatgtaat atggccgacg gcgcggcttg tttacaagcc
      241 gagtgattct tcctgaatcg aagctgcgcc accaacatcc ctgttggtga agtatgtttt
      301 aacttaaaga ttgtcttatt tttaaagctt cggtatggtc agcaggaggc tgaccacgcg
      361 gcctcgattc ttggatgaat cacggccgtc ggccgtcaga atcacctaag aagacggcta
      421 catggaggta ggcgtctgta aaatcgcgaa gcattccgac ggcggtcaag gatgagcgcc
      481 gatatcaggg ggtatccggg ggccaggccc ccggacggcg ggattccaaa gggtgttccg
      541 taacaccctt tggccctaga ggggcggggg agtgggtgac gtcccccgtc gagttcaaag
      601 ccggcatcta tatagttccg gcgagccgga accgagtccg ggtgatattt ttctgaccca
      661 gcgattgaaa gaggccggga gaatcatgtg catcgagttg ttggatcatg tgattaccgg
      721 ggagagggat tattatagct tgagggaaaa agggctgatg tagcgattcc gaggaagagg
      781 aatcagaagt ttaaatagtg aagggtgaag gttgaaaatg aaacagcccc taaaatagaa
      841 atagataatt gaacagccca ggaatggtca gcaggaggcg gaccacgcgg cctagatttt
      901 tgtatgaatt cggcttgttg acaagcgcgc cgtcgaaact cagaattacc caagaagacg
      961 actacatgga ggtggttgtc tgtataattg cgtagcgttc ccacggcgtt cagccgatta
     1021 atcgctatac ttttcatctg gaagggcaag tataggtata gtataataca attatgggag
     1081 acgataggat gcctactttt gaagaaatcc gtcgaaacat tttgcctttc gtagatgaat
     1141 atggaataaa gggaaaacgt aaatcacgga gaaagtaata ggtataaaat ggaattgata
     1201 taaagatttg cctcttcgtc tggcgtttcc gcttccggtt tatccgccgt tataaggaat
     1261 ggtcggttac ccgtaattgc tccaggtata gctgcgtttg tctaagaagt aaaccacgaa
     1321 atctccgatt gattatcatc acatttgaca aggtttaagc aatttaaacc gagtcttaaa
     1381 cccagcaatc aaagccaccg cgactgtgtt taggaagatg caactttcgt ttcaacaaaa
     1441 aactcaaagt aatcccagtc taaccttgta acgaaaggag ggtagtcgat ttttcaagta
     1501 cgatgcagga atgaaatgtc ggcaatcgga atcaccaaaa tacgttaagg aggtgccagc
     1561 atgttttcac cgcaaaaagt cgtattaacc attgtcttag tttgggtgac ggtcttttca
     1621 accaacgtat ttgcagaaac gatctgtttt gatggcccaa tgggatccgc ctgtccgatg
     1681 gaagccctcg accgaggttt gattgcgatg aaagtagaga ccggtgtctt tcttagttgg
     1741 cgattcttaa aaaacgaggt caccggctat tctaccactg gtttaaccgg ggtcaacttc
     1801 aacatatacc gcgatggtgt aaaaatcgca accgtaaccg acagcacaaa ctatctggat
     1861 gattcgggga caggctcatc aaagtattat gtccgcgcca taatcaaaga tatggagttc
     1921 aataaaagcg ccaccgttac tccctgggcc aatccttatt atgatctgcc gctcaatatc
     1981 ccggcggcag ttaccctccc ggacggggta acccccacat attccgccaa cgatatcagt
     2041 gttggcgatg tcgacggtga cggtaaatat gaatactttg tcaaatggga cccgtcaaac
     2101 tcaaaggacg taatccaaaa aggctatacc ggaattgtct atttggactg ttataagcaa
     2161 gacggaacgt tgctttaccg gatcgatatg ggtgtaaata tcagagccgg tgcccattat
     2221 actcaattca tggtttatga ctttgataac gacgggaagt ccgaacttat catgaaaacc
     2281 gctcccggta ccaaaaccgt gcgatacagg ccggacggca gcgtggcatc gcaaagatat
     2341 atcacattgc ccccttggga tatcgccgcc ggttacaaaa ataccgatga ctaccgcagt
     2401 accgacacca gctattatga atatttggtg caagtcttta aggactggcg gagccatccg
     2461 gaggtggtca acggtcattg gccgaatacc ttggaagcct gtttcgggat tgccaatcaa
     2521 tacacttatc ccctgagcga tgccgacgcc aggcaattgg ttgattattt tatggacgtc
     2581 tatgccccat cccgcagtac gagaaacgcc ctgcgcagct ttaccggtta tatctttaaa
     2641 ggccccgaat atttaacggt attcaacggc gccaccggtt cggagatgca gaccattccc
     2701 tataagccgg gccgcgatga cgatggtctg atgtggggcg attatgccat gtcccgaatc
     2761 gagccatgca accgcgtgga acgtatgcag gcctgcgtgg cgtatctcga cgggcaaagg
     2821 ccatatgcgg tgtttggccg cggttattat accaggacta ccttggttgc ctatggctgg
     2881 gatggtaccc ggcttcggga atattggtat gttgacagcg gctatcccat gatgaacaac
     2941 ccctttaaca gcggtcccca tggtatggat ggccggaatg cggcatttgc gacaatcacg
     3001 acccaaggcg ctcacacatt gaccgcggcc gacgttgata acgacggatg cgatgaaatc
     3061 attgccggtg cggcgaccat tgaccataac ggtcatttgt tatacagctc atttgcgccc
     3121 ttgaatggag taaacaccag gctcggtcat ggcgactcca tccatgtcgc cgacatcatc
     3181 ccggacaggc cgggattgga aatattcatg tgtcatgagg ggggcgcctc tgctccttat
     3241 ggtgtatcca tgcgggatgc cgggacaggt gagactcttt gggggacccc taaaagtacg
     3301 gataacgggc gatgtatgat cggtgatgtt tatccgagtt atcgcgggat tgaaagctgg
     3361 tcgatggctt tgcgggccgg agatgcaatc ggtacggaga tcagcaccgt tatgccgggg
     3421 accaatgcca acatcaagtg ggcttcggat ttgaccactc aattcatcga cggttccata
     3481 accgccacgc caaccatcaa gggttggtcg gtggataata caccaaccac catattaact
     3541 tgtaacgata cgtataccaa taattacacc aagggaaatc cctgtttagt agctgatata
     3601 ctcggtgact ggcgtgaaga gctactggtc cggaaagttg acagtagtgc cattcggatt
     3661 tattcgaatt cggagataac cacacacaaa ttgttcacct taatgcaaga ccgccaatac
     3721 cggacggatg tagccagaca aaacgacggt tataaccaac ccgcttacac cagtttctat
     3781 ctggcctttg atatggattg gcaaacgctt taccgtacac tcttaccgta attctgttag
     3841 gacagccccg gttgtaaagc tgggatgagt tcgaattgca ttgaagcttt tcagtttacc
     3901 cttttcacct ttttgatcag agtcccttgg atttgaagtg tatgacgagc acttcggaat
     3961 ctgagggatt tttttaagaa ttccaaggct cagtacagca atagttaact tcaacgctga
     4021 acccggagca gcaccaactt cagacgggag ataactaatc aactaaatga atgcgtaata
     4081 aatatgcttg acgatttgac cggaattatt tataatgagt ataaactcat tgagtttgga
     4141 gtcaattaaa tgaagatttc aaaacgtcaa gcccaaaagg atcagaccag gaaacagctg
     4201 atagaagtgg cttttgagca atttgccagg gatggtttgg ttgccgcgcg caccagcgat
     4261 atcgccgaag ccgccggggt ttcccacgga accgtttttg ctcattttgc gaccagggat
     4321 gatttgctga ttgcggttat tgaagagttc ggcagcaggg ttgccgggcg gattcatgaa
     4381 ttggccggta gcggcggcag tgtccgggag gttttgcagg cccatctgaa tggcctgatt
     4441 gaagttgaac ccttttattc ccggctggtg gccgaaagac tgttgcttcc ttcagaggcg
     4501 cagagcgcct tggtatcgat tcaatccgct attgcaattc atctctatca ggctgtggaa
     4561 ggcgggatca tcgcaggcac gatccggaat atccctttac atttgttatt caacacctgg
     4621 atcagcttaa tccattatta tttgagcaac gccgatctat ttgcgccggg caagtcggtc
     4681 ttaagacgtt atggcccgga actgctggaa cattatttag gattgatcag tttgccgggg
     4741 ggaaatcatg agtaaagact tgaaaatgag gtggtggggg caaagcatat gacgaaaacg
     4801 gttgaaaatc tgttgcgtaa ttccgggctg cccggtccca gaggcaactt gaggctgatg
     4861 tattcatttg ctcatcaagc caccagggat gaaatcaatg aatgtttggc tttttacaat
     4921 gataaattag caaattcacc ggaagaattt gtagtgatgt gcggcatcgt cgggtattgc
     4981 gttttaaatc aaagtcattt gcaagatacg ctcaccgtaa tcaggggtta cgcttcccat
     5041 cagagctggc ggatacggga agcagttgca atggggattc aagaaatcgc gagcgcaaat
     5101 atggaagcaa tcctcgacaa tctcgaaaat tggctgacgg gtgacgagtt ggaaaaacgc
     5161 gccgtggttg cagcattgtg cgaaccgaaa ttactattgg gagaaaaagc cgtcaacctt
     5221 cgaattgtaa atattctggc taaattggcc ctggaatttg ccgaaattga cggcaaactt
     5281 tcagagaatc aaaatgcctt gcgaaaggcg ttgggctacg gctggagcgt ggtgattgtc
     5341 gctatacccg atgaaggaaa ggctgcattt gaagggatgg ccgggaatag caataaacat
     5401 atccaatgga tcgtcaaaga aaacctaaaa aagcaccgtt tgcaggttat ggataaaaaa
     5461 tgggtcgaaa agatgatggc ggtttgtgcc gaaaaagggg acatatccat atgactggtc
     5521 aagtgaaatc aaaaacgaag ttaaaagcca gaacatgtta tggccattta ggcggcaagc
     5581 tgggccaatt gctctttgaa agattggtag aactggaatg gtttactttg gaagagggca
     5641 gggccacagt ttatcaagtc acggaaaagg gtcgtcaaga gcttggcaaa ctgggtgtgc
     5701 ccatcgaata ggggagtgtg aaaacagatg ccgattacac ggtgattgcc ggccggtagt
     5761 gataacaaat atggtttggg aggagaatca taatgaagac ttgtattgct tgcggcatgc
     5821 cgatggtgaa aaaggaagag tttgcaatgg gggatatgtc caaggattat tgcgtttttt
     5881 gcgcccgccc ggacggcagt atgcagtcct atgaggaaaa gttggcagga atgaccgaat
     5941 ttattgtcaa aacccagggg tttgacaaaa acgcggcctt ggaagcggcc cggcaaatga
     6001 tggccaaact cccggcttgg cagggaagga tatagaacta aaaatttatt cgatagcgag
     6061 ctgttttgtt ttaataaagg gaaatgccgc tctgtttccc ttcacttttt caatttaatc
     6121 aaagtctctt ggatttggag tgcataaggc actttggaat ctgaggggct tttttatata
     6181 aatctctgat ttctctgcat cgtgatttaa ttttacgtat agacacaaaa tataactaat
     6241 caacgcaaaa tattacgaga atggaaaaac ggtttcatgt atgattaata ataaattttg
     6301 atatcagcct atattccaag aaaactacat caacaggaat agaggctagg aagaacaatg
     6361 ttccaatggc gccggatgtg caaataaatt taagggggag caaaatgtta gcaaaaggga
     6421 aaggcaccaa tatcaaaacg gtaaggcctt ttggactggc ggataaatta agctacatga
     6481 tgggcgactt tgggtgtaac atgagctttg tgctggtgca atcatacttt atgctgtttt
     6541 atgtaacagt aatggggatt agtccggttc atttcggaat tattatttta attaccaaaa
     6601 tctgggatgg gatcaatgat ccgctaatcg gtgctttaac tgactatctt caacccaagg
     6661 gcggcaagga taaattccgt ccctggattt tgtggacttc agcccccatt accattgcca
     6721 caatcttaat gttcgcctat cttccgggaa taccttattg ggcaaagatt acccaatgtg
     6781 tggtaaccta cttgatttgg gatatctgtt atacctgcgt caacgttccc tatggttcgc
     6841 tgcaatcagt aatcaccgat gatgccgttg agcgcgccga gttatcccgg ttccggactc
     6901 tgggcgcttt catagtacaa ctggcagttt tagcggcctt gccgaagttt atatttgttg
     6961 ataaaaaccc ggtaggaggc cgttttttcc tggcggcaat cattttgagc gtgatgggtt
     7021 ttatcgcctt ccaaatactt tataaattct ccatcgaaag aattaaagtc attgctccgg
     7081 aacaagaagt aaagtataac tatttccgga cttttgcggc attctttaaa aaccgtccga
     7141 tgatggcttt aactatctca tccatatcta cccttttact ggttggttcc accgcagtat
     7201 tgaaccagta tgtatttatc acttactatc aaaatccggg tttattatcg atcggatctg
     7261 taataagtat ggttccggtg ctggtggcaa tgatttttgt gaagatggcg gttaaaaagt
     7321 tcggtaagaa ggccatcagt tcttggccca tattaggagc tgccctttgt tatctttatt
     7381 tactggttgt ccccgttccc aatccgtatg tctggtttgg gttgcaaggt ttggcggcgt
     7441 tgttatcagg attctacacc atgctgacct gggctatggt ggcagactgt atcgattatc
     7501 aagaatggca gacgggccgc agagaagaag gttcgatcta tgctacctac tcgctattca
     7561 gaaagtttgc tcaaggcttc ggcgcttcat tagtagcctt cctgctggca ttcaccgggt
     7621 ataatgccgg gctgcaagca gatcagctcc cgggcgttgc cgaaaacata aagttcctgg
     7681 cggcgctgtt acctttggta ggcaatgtaa ttatcttcct ggcgatgcaa ttgatttaca
     7741 acttggataa taaaggactt gcagaactca aaaagaatct gggacgttga taataattag
     7801 gtaaaacagt ccctgaaaaa ttagggggaa gcttattgag aagaattatc aatattaat
//
LOCUS       DASVMB010000416         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_275380,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000416 DASVMB010000000
VERSION     DASVMB010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275380"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1245)
                     /gene="disA"
                     /locus_tag="VF531_11810"
     CDS             complement(175..1245)
                     /gene="disA"
                     /locus_tag="VF531_11810"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX7714691.1"
                     /translation="MSEDQKRTALFRALKMVAPGTILYEGLANILRAKTGALILISDS
                     ETALKLVDGGFRINTELEPAHLYELAKMDGAIVLSSDAKMILYANAHLTPDHLIPTSE
                     TGTRHRTAERTAIQTGDLVIAISQRRNVITLYKGNERYVIRDLGTMLAKANQALQTLQ
                     KYKSVLEQTLLNLSALEFENLVTLSDVCSVAQRMIMVSRIAAEITRYVYELGTEGRLV
                     NMQLEELMIGVEEEGSLLLRDYLQGELNSVDAIKTEIATWDDDELWDINYFSRVLGFP
                     NGANLDLSVTPRGYRILRKIPRLPLPVVENLVKAYEDLPRILAASTEELDEVEGIGEV
                     RARNIKDGLRRLREQTLFDRHL"
     gene            complement(1318..>2103)
                     /locus_tag="VF531_11815"
     CDS             complement(1318..>2103)
                     /locus_tag="VF531_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7714692.1"
                     /translation="AKQAKIPVILIGQVTKESQLAGPRVLEHLVDTVVYLEGDRSHSL
                     RVARIVKNRFGSTAEVALFEMTDRGMNEVANPSEYLLELENRSHSLPGSVIVATARGT
                     RPLFTEIQALVTDTAEGIPPRQIAIGVDRNRLLLVTAVLQRWVGEHISNKNIFVSTAG
                     GIESDDPALDLGIAASLISSVRGISFHSKIFWIGELTLSGEVRPTPDTPRLLNEGKRL
                     GFEECVMAARVARKDKPDPELQLFGINHLRDLVKLLPALGQKE"
BASE COUNT          545 a          563 c          450 g          545 t
ORIGIN      
        1 catttgaatt taccgtttga cccgctgccg ttcccgacag cttttatata ttagcacagt
       61 tatatcctca ggtcaagtgc tttttttgaa accgcaatct gatcgatgcg ccctgcttaa
      121 agattaccca atagattgaa acattttgag gaatcattca tttttaatat cactttataa
      181 atgtcggtca aacaaagttt gctcccttaa ccgtcgtaaa ccatccttga tatttcgggc
      241 ccgaacctcc ccgatgccct ctacttcatc cagctcttct gttgaagcgg ccaaaattcg
      301 aggcaaatct tcatatgcct ttaccagatt ttcaactacc ggcagaggta aacggggaat
      361 cttccgtaaa atcctgtatc cccggggtgt aacggagaga tctaggttcg cgccgttggg
      421 aaaaccaaga acccgggaaa aataattaat atcccaaagt tcatcatcat cccaagtggc
      481 gatctccgtt ttaatagcgt ctaccgaatt taactcccct tgcaagtaat cccgaagtaa
      541 taaagaacct tcctcctcga ccccgatcat taactcctct aattgcatat taactaatcg
      601 accttcggta ccaagctcgt aaacatatcg ggtgatctcc gcagcgatac gggagaccat
      661 aatcatccgc tgtgcaaccg aacaaacatc ggaaagggtc accaaatttt caaactccaa
      721 agccgaaagg ttgagtaatg tctgttccag cacgctttta tatttttgaa gggtttgtaa
      781 agcctgattg gccttggcca acatggttcc aagatcgcga atgacataac gctcattgcc
      841 cttatataat gtaatgacat ttcgccgttg tgagatggca atgaccagat caccggtttg
      901 gatggcggta cgctccgcag tacggtgccg ggttcctgtt tccgatgttg ggattaggtg
      961 atccggagtc aaatgagcat tggcgtaaag aatcatcttg gcgtcggatg agagtacgat
     1021 tgccccgtcc atcttggcca attcgtacaa gtgagccggc tccagttccg tattgattcg
     1081 gaaccctcca tccaccagtt taagggcagt ctccgaatca gagatcagga tgagagcgcc
     1141 ggttttagct cgtaaaatat tcgccagacc ttcatataaa atagtccccg gggcaaccat
     1201 tttcaatgcc cgaaataacg cagtccgttt ctgatcctct gacaccgctc ctacccccgt
     1261 tcatgagaca ctccgaagtt tttggagatc actctagtgg tatcatgatt cctaatccta
     1321 ttctttctgc cccaatgccg gaagaagttt aaccaaatca cgcagatgat tgatgccgaa
     1381 taattgaagc tccggatcgg gcttatcttt ccgggccacc ctggccgcca tgacgcactc
     1441 ctcaaaaccc agccgtttac cctcattcaa taaccggggt gtatccgggg ttggccggac
     1501 ctcacccgag agtgtcaact ccccgatcca aaaaatcttg gaatgaaatg aaatcccccg
     1561 gacagatgag atcaatgatg ccgcgatccc gagatccaaa gcaggatcat cagattcaat
     1621 gccacccgct gttgaaacga aaatgttttt attgctgata tgttccccta cccagcgctg
     1681 taataccgcc gtgaccagta gtaagcggtt ccgatcgacg ccgattgcaa tctggcgggg
     1741 cggaattccc tcagcggtat cggtaaccaa ggcctgaatc tcggtgaaaa gcggacgtgt
     1801 accgcgagct gttgccacaa taaccgatcc cggcagtgaa tgcgaacggt tctccagttc
     1861 caataagtat tcggacgggt tagccacttc gttcattcca cggtcggtca tttcaaacaa
     1921 agccacttcg gcagtggaac caaatctatt cttgacgatc cgggccactc ttaaactatg
     1981 gcttcgatca ccttccagat agaccactgt atccaccaga tgttccaata cccgcggccc
     2041 tgcgagttga gattccttgg tcacctgtcc aatcaggatc accgggatct tcgcttgttt
     2101 agc
//
LOCUS       DASVMB010000417         5204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_51644,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000417 DASVMB010000000
VERSION     DASVMB010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51644"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VF531_11820"
     CDS             <1..929
                     /locus_tag="VF531_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX7714693.1"
                     /translation="LNPAIDKVYAVDDFTIGGVFRPREMTATAGGKGLNVARVAHLLG
                     EPIIATGFLGGGNGCFIEEQVRQVGITAEFVPIRGETRICIAVMDPKRNTSTEVLEPG
                     PVITEEESQSFLENYRRLLDECVVVTASGSLPRGVPEDGYRKLIAMATERNIRFILDT
                     SGEALKKGIEASPFMIKPNREEAEKLLGAPLATLEDQVEAVLMLKQKGVAIPVITLGK
                     DGCVAGLPDGVYHLYGPAIQVVNTVGSGDSFVAGCAVGVARDLAPIEVIRLGMASAMS
                     NTQFFQTGMVSPESVSRFFEVIRYRLIGPFSH"
     gene            1168..2046
                     /locus_tag="VF531_11825"
     CDS             1168..2046
                     /locus_tag="VF531_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7714694.1"
                     /translation="MEAVKPHEISGHGLLLIRSHLNSLKPAERRVAEYILENSMLVVR
                     LSITKLAEEAGVSEATIIKFCQRVGYPGYQELKISLAQEMGEGCSEQGIYGPIQADED
                     IETVIEKIFQIYNRSLNDTKRLLLKAPISQAVRKVLGAGRIYFFGYGASGIVAQDAEI
                     KFKRINRTAEAFTDNHMQKTMAALLKPDDLVVAISDSGRTKELVEALEIAKPTGACII
                     AITSNMGSPLSKLADLVLLTSVTETPFRGSALASRMVQLAIIDVLFLGAATADYQNTV
                     EFLNKTRMALLNNKLD"
     gene            2059..2964
                     /gene="murQ"
                     /locus_tag="VF531_11830"
     CDS             2059..2964
                     /gene="murQ"
                     /locus_tag="VF531_11830"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006072912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX7714695.1"
                     /translation="MAVTKGVTRITEQRNPLTMDIDLLNTGEILGLINDEDAKVAAAI
                     RAVLPAITQAVDLIAARMKAGGRLFYIGAGTSGRLGILDAVECVPTFRIEPDRVVGIM
                     AGGKDAIFRAKERVEDDYSLGSQEIAVHQINANDSVVGIAASGRTPYVLGALAEARGR
                     GAATVGLFCSEDPAAASCADVIIIPIVGPEALTGSTRMKAGTAQKMVLNMISTTVMIK
                     LGKVYSNLMVDLNPSNSKLRERAKQIFMTITGATAETAADYLKRSADNVKAAVVMYER
                     RCSVEEAIRLLQQADGFLRKVIGKK"
     gene            3021..4283
                     /locus_tag="VF531_11835"
     CDS             3021..4283
                     /locus_tag="VF531_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714696.1"
                     /translation="MRKWIMILACLVLVCTLFLGLAGVGSAAAKKDVTLTFWSWRTED
                     VDAYTKFIQVFNKKNPNINVKFIPYKNTEYNTILSTALQGGSGPDIMQLRAYGGMEPL
                     ANAGYLRALDGLVPELKGFDTNVLQGARSRKDQKIYGVPFAVQSIQVLYNKKIFTKLK
                     LKVPTTWGQMLKVAQTVKNAGLIPFANGTKDAWTLETMFGGVAPTFYGGDELNNQIVK
                     GQTNFEDARLKQALSKMLELRPYLPDNYTGVGYTDMQMMFAQEMAAMFIAGSYELGTM
                     AQMNPNLEIGAFIVPGETASAPKFNSTYVDGSYGINAATKYPDEALKFIRFLAGRQYG
                     QLFTDNLKQISAVPNIKSKDAALNGIIKVMKPTPYLMLTGFRYGTPSGSTLLQNEVQA
                     LFAGAQDIDKTLKNIQTGVATWYEPFKK"
     gene            4405..>5204
                     /locus_tag="VF531_11840"
     CDS             4405..>5204
                     /locus_tag="VF531_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714697.1"
                     /translation="MRRWLARHIYVAFILPGLLLYGVFMVYPLLSAMVYSVFRWDGLL
                     RSGFAGLENFKTVLTGVAYQPRFWGALGHNAIFFAFTLTLQTTIGLYLAVQLTKERKG
                     TAAFQTIYFIPYTLSMVVVGFLWLQLLNPTWGAIPRGLRTLGLAGWVHPWLGDTGTAL
                     ITVILVNFWRWVGFPVLVFKAGLQSIPKELREAAAVDGATKTRLFRYITLPLLIPTIM
                     MMSIMTFIWDFNAFDLIFVMQGSSGNPHYATDVLATLFYRTAFGDSTTG"
BASE COUNT         1293 a         1215 c         1427 g         1269 t
ORIGIN      
        1 atctaaaccc cgcgatcgac aaggtttatg cggtggatga ctttacaata ggtggtgtct
       61 tccggccccg tgaaatgacg gccacggccg gaggcaaagg gttaaacgtg gccagggttg
      121 ctcatctttt gggtgaaccg ataattgcca ccggtttttt aggcggtggc aacggatgct
      181 tcatcgagga acaggtacgt caggttggaa tcacggctga atttgtgccg atacgtggag
      241 aaacgcgaat ctgcattgca gtaatggatc cgaagcgcaa tacatccacg gaggttttgg
      301 agccgggtcc ggttattacg gaggaagaat ctcaaagttt tctcgaaaac taccggcggc
      361 ttttggatga atgcgtggtg gtcaccgctt cgggaagtct tccccggggg gtgccggaag
      421 atggttatcg gaagttaatt gccatggcaa cagagcggaa catccgtttt atcctggata
      481 ccagcggtga agctctgaaa aagggcattg aagcctcacc tttcatgatc aagcccaacc
      541 gagaggaggc cgagaaactt ttgggggctc cactggccac cttggaggat caagttgagg
      601 cggttttgat gctgaagcaa aaaggagttg ccatccccgt tattactttg ggaaaagacg
      661 gctgcgtggc gggactgccg gacggagttt atcatttata cgggcctgcc attcaggtgg
      721 ttaacaccgt gggttccgga gattcattcg tagcgggttg cgccgtaggg gtggcgcgtg
      781 accttgcccc gatagaagtg attcgactgg gaatggcttc ggccatgtca aatactcagt
      841 tttttcaaac cggaatggtc tctccggaaa gcgtctcccg gttttttgag gtcatccggt
      901 accggctaat cgggccgttt agtcattaag ccatcgtaat ccagcaaacc gttcctgtgg
      961 aacggttttt tgttttcatc actggaaata gagggttaac caaggctagc caatattaac
     1021 tgaagaggct cgccgtttga aaagagagcg ggaaagtttc ctttgccagt aataaatttt
     1081 ttttgttata tttaccattc taagaaggaa tttttactat ggcaacaaat attgtttata
     1141 ggtgatcatc aattaggagt gaaaccgatg gaagcggtta aacctcatga gatttcgggc
     1201 cacggactgt tattgatacg tagccacctt aactccttaa aaccggccga gagaagagtg
     1261 gccgagtata ttctggaaaa ctcgatgctg gtggtgaggc tatccatcac caaactggct
     1321 gaggaagccg gagtgagtga agccaccatt atcaagtttt gtcaaagggt tggttacccc
     1381 ggctatcagg aactcaagat ctcgcttgcc caggagatgg gggaaggctg ctcggaacag
     1441 ggaatttatg gcccgattca ggccgacgag gatattgaaa cagtaatcga aaagatcttt
     1501 caaatttata accgttcgtt gaacgatacc aagcgtttat tactcaaggc tcccatctcg
     1561 caagcggtcc ggaaagtatt gggagccgga cggatttatt tcttcggata cggggcctcg
     1621 ggaattgtag cccaggacgc cgagatcaag tttaaacgaa tcaaccggac agctgaggcg
     1681 tttaccgaca atcatatgca aaaaaccatg gctgcgctat taaagccgga cgatctggtg
     1741 gtggccatct cggactcggg gaggaccaag gaattggtgg aggcgctaga gattgccaag
     1801 ccgaccgggg cttgcatcat cgccatcacc tccaatatgg gttcgcccct ctccaaactt
     1861 gcggatctag ttcttttgac ctccgttacg gagacaccgt ttcggggcag cgcgctcgct
     1921 tcacgtatgg tgcaactggc tatcattgat gtgctgtttc tgggagccgc tacagccgat
     1981 taccagaata cggtagaatt cttaaacaaa accagaatgg ccctgttaaa caacaaactg
     2041 gactgagggg gtaacaaaat ggctgtaacc aaaggggtta cccggatcac ggaacaaaga
     2101 aatcctttaa ccatggatat agatctctta aacaccgggg agatcctggg cttaatcaat
     2161 gatgaagacg cgaaagtagc tgcggcgatt agggcggttt tgcctgcgat cacccaagcg
     2221 gttgatctca tcgccgccag gatgaaggcc ggaggacgtc tgttttacat aggtgccgga
     2281 accagcggcc gtctggggat cttggatgcg gtcgaatgtg tcccgacctt ccggattgag
     2341 ccggatcgag tcgttggaat catggccgga ggcaaggacg caatcttccg tgccaaggaa
     2401 agggtagagg atgattacag cctgggatcc caagaaattg ccgttcatca aatcaatgcc
     2461 aatgattctg tggtagggat tgcagccagc ggccggaccc cttatgtctt gggcgccctg
     2521 gctgaagccc gcgggagggg agccgccaca gtgggtctct tttgctctga ggatccggcc
     2581 gccgcatcct gtgccgatgt aataatcatc cccatcgttg gaccggaagc cctgaccgga
     2641 tccacccgga tgaaggcggg gaccgctcaa aagatggtgc tcaacatgat ctccaccacg
     2701 gtcatgatta aattgggaaa agtctattcc aatctgatgg tcgatttgaa tcccagcaat
     2761 agtaagttgc gggaacgggc caagcagatt ttcatgacaa ttaccggtgc aaccgccgag
     2821 actgcagcgg attatctcaa gcgatccgca gataatgtga aggcagcggt ggtcatgtat
     2881 gagaggaggt gttcagtaga agaggcaatc cggcttttac aacaagcaga cggattcctg
     2941 cgaaaagtga tcggcaagaa ataggttcat tcgcaatcca gttttaccaa ggaatgaata
     3001 agaggagggg aagtatcaag atgagaaagt ggatcatgat tttggcttgc ttggtgttgg
     3061 tttgcacgtt atttttaggt ttggcgggag tcggatcggc cgcggctaaa aaagatgtca
     3121 cactcacctt ttggagctgg cgtactgagg atgtcgacgc ctataccaag tttatccaag
     3181 tatttaacaa aaagaacccc aatatcaacg taaaattcat tccatacaaa aacacggagt
     3241 acaatacgat cctttctacc gcgctccagg gcgggagcgg gccggatatc atgcagctcc
     3301 gggcctatgg cgggatggaa cctttggcca acgccggata tctccgggca ctcgacggct
     3361 tggttcctga gttgaaaggt tttgatacca atgtattgca gggggcccga agccggaagg
     3421 accaaaaaat atacggagtc cccttcgcgg ttcagtccat tcaggtgctt tataataaga
     3481 agatctttac caagctgaaa ctgaaggtgc cgaccacctg gggtcagatg ctaaaggttg
     3541 cccagactgt gaagaacgcg ggtttaatcc ccttcgccaa cggcaccaag gacgcttgga
     3601 ccctagaaac catgttcggc ggtgtggcac cgactttcta cggcggggac gagctgaaca
     3661 accagatcgt taaaggccaa accaattttg aggacgcccg gttgaagcag gcattaagta
     3721 agatgctcga gctccggccc tatctgccgg ataattatac cggtgtgggt tataccgata
     3781 tgcagatgat gtttgcccag gagatggcgg caatgttcat cgccggttcc tacgagctgg
     3841 gtaccatggc ccaaatgaat ccgaatctgg agatcggtgc ttttatcgta cccggtgaga
     3901 ccgcaagcgc acccaagttc aactcgacct atgtcgatgg atcctatggc atcaatgctg
     3961 ctaccaaata ccctgatgaa gccttgaagt tcatccgttt cctggccggt cgccaatatg
     4021 gccagttatt cacggataat ttgaaacaaa tctcggcggt gccgaatatc aaatcgaaag
     4081 acgcggcatt gaacgggatc attaaggtaa tgaaacccac tccctacctc atgctaactg
     4141 gtttccgtta cggaacacct agcggttcca ccttgctcca gaacgaagta caggcattgt
     4201 tcgccggtgc tcaggacatc gataaaacgt tgaagaacat tcaaaccggg gtggcaacct
     4261 ggtacgaacc gttcaagaaa taaccttgtt ttgtgacggg gctgtctcaa aaggaacgat
     4321 tgtcttcaac agaacgacaa aaagcttctt ttgagacagc ctcgttcttt tggaaggcaa
     4381 ctttgaatcc gggaggctgt gatcgtgaga agatggctcg cgagacatat ctatgtcgct
     4441 tttatcttgc ccgggctgct gttatatggg gttttcatgg tttatcctct attatcggcc
     4501 atggtttact ccgtttttcg ctgggatgga ctgctgcgaa gcggttttgc cggactcgaa
     4561 aattttaaga ccgtacttac cggtgtggcg tatcaaccta gattttgggg ggcattgggg
     4621 cataacgcga tcttttttgc gtttacattg acgctccaga ctacaatcgg actgtacctc
     4681 gccgttcagt tgaccaagga acgaaaaggg acggcggcat tccaaacaat ttacttcatt
     4741 ccctatacgc tatcaatggt agtggtagga ttcctctggc tgcagttact caaccccact
     4801 tggggagcga ttccccgggg actgagaact ctcgggctgg caggttgggt gcatccctgg
     4861 ttgggagata ccggcaccgc cttgatcacc gtcattttgg tgaacttctg gcgctgggtg
     4921 ggctttccgg tgttggtttt taaggccggg ttgcagagca tccccaagga attgcgtgaa
     4981 gcggctgcgg tggatggagc cacaaagacc cggttattcc ggtatattac cttgccactc
     5041 ctcattccaa ccatcatgat gatgtcgata atgactttta tctgggattt caatgcattc
     5101 gatctgatct ttgtcatgca gggttccagc ggcaatcccc attacgccac cgatgtgctg
     5161 gcaactttat tttaccggac cgctttcggt gactccacta ccgg
//
LOCUS       DASVMB010000418         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_420911,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000418 DASVMB010000000
VERSION     DASVMB010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420911"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..903)
                     /gene="phoU"
                     /locus_tag="VF531_11845"
     CDS             complement(196..903)
                     /gene="phoU"
                     /locus_tag="VF531_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7714698.1"
                     /translation="MEIFEHALQRERKFMLKHQNAYDRALLQIRNRIGNLAGLVEERL
                     CQAMSSLVQQDNILAGRILQGDDEVDSVVEALERDCLDLILLQQPTDSDLRFLSAATR
                     IGREMERIADYACDIAELTLELKQKVPYFKPLVDLPRMGEMVADMLRQSIKVFLDQDL
                     AGARRVVESDDPVDELFTALLQELTDYMKKGPEYVDQASRLLLVARYLERIGDHVVNI
                     AEMVVFIATGERHPFKS"
     gene            complement(1035..>1620)
                     /locus_tag="VF531_11850"
     CDS             complement(1035..>1620)
                     /locus_tag="VF531_11850"
                     /inference="COORDINATES: protein motif:HMM:NF020476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HEX7714699.1"
                     /translation="DYPRFIEPKVPDLNRRLATFQSLAERLGPARVIWRYDPIIFSNL
                     TPLEYHLEHFAELAARLRGYTGRVIISLLDDYPRIRKRIIRLREEAGWEVTQAAALNL
                     EGFTAALSRTATTNGMTIQSCAEPLIQAEGGIQPGACIDPVLLNRLFGLNLTQAKDKY
                     QRPDCGCAAAVDIGTYNTCGMHCVYCYANAGSSR"
BASE COUNT          406 a          463 c          379 g          372 t
ORIGIN      
        1 tagccgccaa ggcaccaatc cctaccgcca cgattggatt acccgttaaa accttaaccc
       61 attgtttcaa gcgccgggcc gcaagtttct gaagaccttc ggaacatagc tgcattccga
      121 aaagaaaaag gcccagacct ccggccagac ccagtaacga aaaaatgaag ctcatgtctc
      181 accctcgcca aaattttacg acttaaacgg atgcctttcc ccggtggcga tgaataccac
      241 catttcggca atattgacca catgatcgcc gatccgctcc agataccggg caaccaaaag
      301 caaccggctg gcctgatcca catattccgg gccctttttc atatagtccg tcaattcctg
      361 gagtagggcc gtaaagagtt cgtcaacggg atcgtcactc tccactaccc tgcgggcacc
      421 ggccagatct tgatccaaaa agaccttgat actctggcgc agcatatccg cgaccatttc
      481 tcccatccgg ggcagatcaa ccaaaggttt gaagtatgga accttttgtt tcaattccag
      541 agttaattcg gcgatatcgc aagcgtaatc ggcaatccgt tccatctccc ggccaatccg
      601 cgtcgcggcg gataggaaac gaagatcgct atcggtaggc tgttgcaata ggatcagatc
      661 caggcaatcc ctttccagcg cttctacgac cgaatccacc tcatcatcac cctggaggat
      721 ccgcccggcc aagatattgt cctgttggac caatgaactc attgcctggc ataaccgttc
      781 ctcgaccaaa cccgccagat tcccgattct gtttcttatc tgcaataaag cccggtcata
      841 ggcattttga tgtttcaaca taaacttccg ctcccgttgt aaggcgtgct caaatatctc
      901 cacaaaataa gccgccgtgc tcataacgaa accttaaaac atgcagtccc aaatctgtaa
      961 gttacgtttt atcatacctt ttttttgcga ttcctgcaac cggagaatgg tagctccgtc
     1021 tcttagcagc acagttaccg ggaagaaccg gcgtttgcat agcaatagac acaatgcatc
     1081 ccgcaggtat tataagttcc gatatccact gccgctgcac agccgcaatc aggacgctga
     1141 tacttatctt tagcctgagt cagattgagg ccaaaaagcc ggttgagaag gaccgggtca
     1201 atacaagccc cgggttgaat cccaccttca gcttgaatca gcggttcggc gcaactttgg
     1261 atggtcatcc cgttggttgt cgcagtccgg ctcagtgcgg cggtgaaacc ttccagattt
     1321 aaagcggcgg cctgggttac ttcccaaccg gcctcctcgc gtaaacggat aatacgtttt
     1381 ctaatccggg gataatcatc tagcaggctg atgatgaccc gcccggtata gccccggagc
     1441 cttgcggcga gctcagcgaa gtgctccaga tgatactcaa ggggtgtcag gttgctgaaa
     1501 atgatcgggt catagcgcca gattacccgg gccgggccca gcctctcggc gaggctttgg
     1561 aaggtggcga gacgccggtt taaatcgggc accttgggtt caataaaacg ggggtaatca
//
LOCUS       DASVMB010000419         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_420924,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000419 DASVMB010000000
VERSION     DASVMB010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420924"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1420)
                     /locus_tag="VF531_11855"
     CDS             complement(59..1420)
                     /locus_tag="VF531_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7714700.1"
                     /translation="MRKKQIETDSRVKASHFTWKEFALTYSVLLVLSTGQGLIFATYM
                     DFDTTPPGYIVEMLGYWATVTAIFCLVTNRQRKRFFDKPMRKLSEAAGRVAEGNFSVY
                     LAPDHTAEKVKYFRWKEFVQTYFVLLALSWGQEMILAEYMDFDSPPPGYIGGMLGYWA
                     IVTAIYCLVTNRQRNRFFDKPMRKLSEAAGQVAEGDFSVYLEPAHTADKLDYVDVMFE
                     DFNKMVEELGSIETLKNDFIANVSHEIKTPLSVIQSYAMALQKENLSAEQRKEYTDTI
                     ITASQRLTTLVTNILKLNKLENQEIKPAAEAYDLCRQLCDCALNFEDLWEKRNIQFVA
                     DLDDRAIIRADESMLEIAWNNLLSNAFKFTDPGGTVTLRQTSDEDTVTVMISDTGCGM
                     SERTMQHIFDKFYQGDTSHSQEGNGLGLPLTLRVIELSGGTISVSSELGKGTTFTVKL
                     KVD"
     gene            complement(1417..2088)
                     /locus_tag="VF531_11860"
     CDS             complement(1417..2088)
                     /locus_tag="VF531_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714701.1"
                     /translation="MIHILVVEDDIKLNQIVCTYLCDNGYAAKGCLNPREAYDLMYNN
                     PYDLIISDIMMPEIDGFEFAETVRDVNKSIPILFMTASDDISSKRRGFRAGIDDYMVK
                     PINMDELVLRVGALLRRANIANEKRLTVGGLVMDADAMTTTVNGEEIPVTVREFNILY
                     KLLSYPKHTFSRAQLMDEFWGVESETSLRAVDVYITKLRDKFSGCEEFKIVTVHGLGY
                     KAVLQ"
     gene            complement(2503..>3621)
                     /locus_tag="VF531_11865"
     CDS             complement(2503..>3621)
                     /locus_tag="VF531_11865"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEX7714702.1"
                     /translation="LKFTKYNQYESLIKPMLKYLESHGVQFQYDTKVTNIVFDIKNGK
                     KLAKKIVYERSGKEESIELTKDDLVFVTNGSCTENSTLGDQNHAPEFNDSIGGCWQLW
                     RNIAQQDPALGNPDKFYTDTKKTNWESATITTLDNKIPPYIERICKRDPFSGKVVTGG
                     IITVKDSKWLMSYTVNRQPHFKEQPKDQLLVWVYGLFTDVPGDYVKKPMRECTGLEIT
                     EEWLYHMGVPVYEIHELAAKSARCVPCMMPYITAFFMPRTKGDRPKVVPDGSENFAFI
                     GQFADTVRDTVFTTEYSVRTAMEAVYTLLDVDRGVPEVFGSCYDIRVLLDSTVKMMDG
                     KKPGDMKLPFMLNLVRKKALKKISGTAIEEVLKRYNVI"
BASE COUNT          911 a          899 c          767 g         1044 t
ORIGIN      
        1 aaacaaaaaa gaacagcctt ccgattcggg aaagctgttc tacttctatt gccggtaatt
       61 aatcgacctt tagcttcact gtgaatgtag ttcctttgcc caactcactg gagaccgaaa
      121 ttgtgccgcc tgaaagctcg atgaccctga gtgtaagggg aagacccaag ccgtttcctt
      181 cctgtgaatg agaggtgtcg ccctgataga atttatcgaa gatgtgctgc atggtccttt
      241 cgctcatgcc gcagcccgta tcggatatca tcactgtcac ggtatcctcg tcagaggttt
      301 gtctcagcgt gacagtgccg ccgggatcgg tgaatttaaa cgcgttggag agtaggttat
      361 tccatgcgat ctctaacata ctctcgtcgg cgcgaatgat ggcgcggtca tccagatcgg
      421 ccacaaattg gatgtttctc ttttcccata agtcttcaaa attgagcgca cagtcacaaa
      481 gttgtcgaca gagatcgtaa gcttcggcgg ccggcttaat ctcctgattt tcgagtttat
      541 tgagtttcag gatgttggtc accagcgttg tcagccgttg cgatgccgta ataatggtgt
      601 cggtgtattc tttgcgctgt tcggcggaga gattctcttt ttgcagcgcc attgcgtagc
      661 tttggatcac ggacagcggc gttttgatct catgagacac attggcgatg aagtcgttct
      721 tcaatgtctc gatactgccc agctcctcca ccattttatt gaaatcctca aacattacat
      781 ccacataatc caatttgtcg gcggtgtggg caggctcaag atagaccgaa aaatcgccct
      841 cggccacctg ccccgcagct tcactgagct ttctcatggg cttatcaaag aaacgattcc
      901 tttggcggtt tgtgacaagg caataaattg cggtaacaat cgcccaatac ccgagcattc
      961 ctccgatgta tcccggaggg gggctgtcaa aatccatgta ctcagcaaga atcatttctt
     1021 gcccccaaga taatgccaac agtacaaaat aggtctgcac gaactccttc catctgaaat
     1081 acttgacttt ttcggcggtg tgatcagggg ccagatagac cgaaaaattg ccttcggcca
     1141 cccgccccgc agcttcactg agttttctca tgggcttatc aaagaaacgt ttcctttggc
     1201 ggtttgtgac aagacaaaaa attgcggtaa cagtcgccca atacccgagc atttcaacga
     1261 tatatcccgg aggggtggtg tcgaaatcca tgtacgtggc gaaaatcaat ccttgccccg
     1321 tagataatac caatagtacc gaataggtca gcgcaaattc cttccatgtg aaatgactgg
     1381 ctttgacccg gctgtccgtt tcgatttgct tctttctcat tgcagtaccg ccttatagcc
     1441 aagtccatgt acagtgacga tcttgaactc ctcgcagccg gaaaacttat cccgaagctt
     1501 cgtaatatac acatccacgg cgcgcaggct ggtttcgctt tcgacgcccc agaattcatc
     1561 cattagctgc gcgcgggaaa aagtgtgctt gggataggac agcagcttat aaagaatatt
     1621 gaattcacgc accgtaaccg ggatctcctc accgttgacc gttgtggtca ttgcgtccgc
     1681 atccatcaca agaccaccta cagtcagacg cttttcattc gcgatattcg cccggcggag
     1741 cagagccccc acgcgcagca ccagctcgtc catattgatg ggttttacca tataatcgtc
     1801 aatgccggcc cggaaacctc ttcgctttga ggaaatatcg tcactggcag tcataaacaa
     1861 aatgggaatc gacttgttga catcccgcac tgtttcagcg aactcaaaac catctatctc
     1921 cggcatcata atgtccgaaa tgatcagatc atacgggttg ttatacatca aatcatacgc
     1981 ctcccgcggg tttaaacatc ccttcgccgc gtatccgtta tcacacagat aggtgcatac
     2041 gatttgatta agctttatat catcttcaac tacgagaatg tgaatcacgg ttttctcctt
     2101 cttacctcga ttttatcgga ttttcctttt taattaatcc gatagtccgg attaggtttt
     2161 attataaaag acgactgtta gataaatgtt aaacagggcg gtactcaatc cgaatatact
     2221 acattcatcg acattttctt tactaaattc gatatgttga gttgagatga tattatatcc
     2281 ctgccgaaca tagaaatata atttcttgag tccccaggac ttgaatcgtg caaatgaaat
     2341 catcccagtt ttaaagaaca aaaagacggg ggaactcgtc tcccgtcttt taccccaaat
     2401 aatatgttaa ttattccgtc caagcttttt ctctggagag aattgatccc caaggtttat
     2461 ttccctttag actccaattc tctcattttc cttttatcct ggttagatca cgttatatct
     2521 cttcaaaact tcttcaatag ccgttcctga aatcttcttc aacgccttct tcctgaccag
     2581 attcaacata aatggcagtt tcatatcgcc gggcttttta ccatccatca tttttaccgt
     2641 ggaatccagc aatacacgga tatcgtagca tgaaccaaaa acctccggca cgccacggtc
     2701 tacgtccagc aatgtgtaaa ccgcctccat ggctgttctc accgagtact ctgttgtaaa
     2761 caccgtatcg cggaccgtat cggcgaactg gccgataaag gcaaaatttt cgctgccgtc
     2821 aggtacaacc ttaggtctgt cacctttggt tctcggcata aagaatgccg taatatatgg
     2881 catcatgcaa ggaacacagc gggcagattt cgcagccagt tcgtgaatct cgtatacagg
     2941 aacacccata tggtaaagcc attcttcagt gatttccagg ccggtacatt cccgcatcgg
     3001 ttttttgaca taatctcccg gcacatcggt gaacaaacca tatacccaga ccagcagctg
     3061 atctttaggt tgttccttaa aatggggttg ccggttcacg gtataactca taagccattt
     3121 tgaatctttc accgtgatga tcccgccggt aacaaccttg ccgctgaatg ggtcgcgttt
     3181 acagatcctc tcgatatatg gaggaatttt attatcaagc gtggtaattg tggcggactc
     3241 ccagtttgtt tttttagtat cggtatagaa tttatccggg ttaccgaggg ccggatcctg
     3301 ttgcgcaatg tttctccaaa gctgccagca tcctccgatg gaatcattga actccggtgc
     3361 gtggttttga tcaccgagtg tagaattctc cgtgcagctt ccgttggtaa caaacaccag
     3421 atcgtctttg gttagttcaa tggattcttc tttgccgcta cgttcgtata cgatcttttt
     3481 agcaagcttt ttgccatttt taatgtcaaa aacaatgttt gttactttcg tatcgtattg
     3541 gaactgaacc ccgtgggatt ccagatattt taacattggc ttgattaatg actcatattg
     3601 attgtatttg gtaaacttaa g
//
LOCUS       DASVMB010000420         8152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_715701,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000420 DASVMB010000000
VERSION     DASVMB010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715701"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..1300)
                     /locus_tag="VF531_11870"
     CDS             complement(431..1300)
                     /locus_tag="VF531_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7714703.1"
                     /translation="MELSQLRSFRLVAEQRSFSKAATILHLTQPAVTQQIKNLEIELG
                     EILFERSGRQPSLTQAGEALLTYAQQILNLAGTATETIRQFSRIRGRLTIGAGTTNTI
                     FRLPQILQTYRRSYPQVEIRIRNGDSELISRLVWENSVDLGLITTINPDLHLQSIPLF
                     EDRIFLVAPTGHPQSLSPAELAGESLILFRSGSGFRRFLEDHFQASRFTPQVIMELES
                     MEAIIRMVASGLGLAFLPEVAVHGELERGELVRVRIEGWPKLARQTFLIYRRDKHLSW
                     PVEALLGQLNGES"
     gene            1523..2812
                     /locus_tag="VF531_11875"
     CDS             1523..2812
                     /locus_tag="VF531_11875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7714704.1"
                     /translation="MPTVVVVGLQWGDEGKGKITDFLASQADMVVRYQGGNNAGHTVV
                     VGDEEFKLHLVPSGILYSGTLCILGNGVVLNPGVLLKELDELKSRGVDVSGIRVSDRA
                     HLIMPYHPIFDQMEETNRVVGKIGTTGRGIGPAYVDKYARYDAIRVVDLLDPEGFREQ
                     LERIVAVKNESFEKIYGSPALNWRTVYDEYLQYAEALRPMVADTSVLVNQAIKKRQKI
                     LFEGAQGALLDIDHGTYPFVSSSNPTAGGACNGSGVGPTKIDKVVGVIKAYSTRVGEG
                     PFPSELSEDLVSQLRDGRGREYGVTTGRPRRCGWFDGVIAEYSTRINGVNALIVMKLD
                     VLDKLPTIQICTGYRYRGSILKEFPASLKVLAECTPVYEELPGWQQDTTGARELGDLP
                     LKAREYLNRISDLAQTPIAMVSVGWRRDETIMIENIW"
     gene            2900..2975
                     /locus_tag="VF531_11880"
     tRNA            2900..2975
                     /locus_tag="VF531_11880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2933..2935,aa:Lys,seq:ttt)
     gene            3072..3147
                     /locus_tag="VF531_11885"
     tRNA            3072..3147
                     /locus_tag="VF531_11885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3106..3108,aa:Glu,seq:ttc)
     gene            3514..3708
                     /locus_tag="VF531_11890"
     CDS             3514..3708
                     /locus_tag="VF531_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714705.1"
                     /translation="METIKGAIGVIASLHLSWLTLYKIGNTWYNKFKISGKGGIFAMG
                     DKSPKNSEKLKKNADKKTKK"
     gene            3903..4109
                     /locus_tag="VF531_11895"
     CDS             3903..4109
                     /locus_tag="VF531_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714706.1"
                     /translation="MARIFGDRRVNPNWDSRLTTNQRALLTTPGFGIVEKLPYLPEED
                     VKKIMDIIEKSIEQVGSMINASRQ"
     gene            4667..5341
                     /locus_tag="VF531_11900"
     CDS             4667..5341
                     /locus_tag="VF531_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX7714707.1"
                     /translation="MPYTAYIEEAGDEGLGKGSRWFIVGAVIVNKSKDLAVSRCIDEI
                     KHKFKLQPPNGPIHWRNIKHTSIRRGIIECLAGHEFNISYVICDTYHDEIMNSTLKGS
                     GRLYFDLARVLIERISWFGAERADTVDLVFANRFNMLYSDLKDYLTLLLNDRTCQIKP
                     GIIDLNRISCQNVGQLKLLQVSNICNSSCFNALEFDQYGYYDERFLLGLSNKFSGVKV
                     ISLVTV"
     gene            5618..6073
                     /locus_tag="VF531_11905"
     CDS             5618..6073
                     /locus_tag="VF531_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714708.1"
                     /translation="MSFLVLEDDELDFFWNSIQDIQVIFHPTYAPEGMFNYQELSNLK
                     NKKDVIVFLDRNLLSSLLKLCEQGNLTDEKEKNTIAKVMLWTLGNNFSVSAGLALKEN
                     ASKTNNSEYAKKELQRFKEIIEYYPIMMWLRLAEGVMDSKSPKPNQELF"
     gene            6486..>8152
                     /locus_tag="VF531_11910"
     CDS             6486..>8152
                     /locus_tag="VF531_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX7714709.1"
                     /translation="MDLNNFENYIDPKIVARGYDYYENDYVTSVEEIKENVFVAKVEG
                     TDRYTVEVELDHQANIIDTQCDCPYDLGEYCKHQAAVFLTLREMKNNISDEKSPALKH
                     HCMDLEAAFKSPVRKKRKEIDIKKVLSDRTKDELVAFLLDIASEYEEIKRRIELNFYI
                     GNDEDEINKSIALIRTYINNNSNEWGFVGYGNTSEAVKGADMVLEKARYALERSKTTH
                     ALKLALCVVQEMIDLLQGADDSDGVIGGTIEESFAFIAEIIADEELSPVQKEDIFDQL
                     MTEAANKRYDGWIDWRLDLLAKCSELADNRHLRNKLENRLVSMIENENGDSWSINYFA
                     EQINLIRYQMIEKYDGRKKAEEFIEQNLQYSSFRKKAIESAMKKKDYNEVVKLAIDGE
                     EKDKGSPGLADQWKGYRYKAFKLSGMLDEQRRIAVDFILKGDFEYYQELKNTYDTKEW
                     PSVYPEIILRLENQKKTHQDVYTRILIEEGEKQKLCEYVKGRPSTVECFCKYLIPEFT
                     EDVYGLFRQYIEQTAARANSRKDYRGVCAIIRNLKKAGGKQPALEIK"
BASE COUNT         2399 a         1614 c         2001 g         2138 t
ORIGIN      
        1 tgaagtgcgc cgtaacggaa cgccgatcaa tcccctgaag tttctgcctt aagataatgg
       61 aaggagcccg gctgaaccaa accgggctcc tgggtggaaa acactcactt taccgaaggg
      121 ttacttactt cccttccaac cattcagttg ttggtccttt gactcatccc ggtcaaagga
      181 gagacgatgg accaaagtaa aggttggaga ggccggcagg atcatccgac cgtatcacag
      241 gcccgacaat cgcgcgggta aaccgtgcgc gggctagcag tcacctttga tgaacctgag
      301 gctcaacgag ccatgatttg ccgtctcttt actaccggcc tcttcgttac atagtatatg
      361 accgaaaacc aagaatatac gaaataatcc tttccatatt taggaatatg caaatcattt
      421 ttcgaaatcc ttaagactcc ccgttcagct gacccaggag ggcttcaacc ggccaggaaa
      481 gatgtttgtc gcgccggtag atcaaaaagg tctgacgggc caatttcggc cagccctcga
      541 tccttacccg taccaattcc ccgcgctcca attcaccatg aaccgcaact tcaggcagga
      601 aggctaatcc caggccactt gcgaccatcc ggatgatagc ctccatactt tcaagttcca
      661 tgatgacttg cggcgtaaac cgtgaagcct gaaagtgatc ctccaggaaa cgccggaaac
      721 cggagccgga gcggaagagt atcaatgact ccccggccaa ttccgccggg gaaagacttt
      781 gagggtgacc ggtcggcgcc accagaaaga tccggtcctc aaataaagga atgctttgca
      841 gatggagatc cgggttaatg gtggtgatca aaccgagatc aaccgagttt tcccaaacca
      901 gacgggagat tagttccgaa tcgccgtttc gaatcctgat ctccacctgg ggataggacc
      961 gtcggtaggt ttgaagaatt tgggggagtc gaaagatggt attggtagtt ccggcaccga
     1021 tggtcaagcg gccacggatt cgggaaaact ggcggatcgt ctccgtagcc gtcccggcca
     1081 gattcaaaat ttgctgggca taggtcaaca gcgcctcacc ggcttgggtt agggaaggtt
     1141 ggcggccgga acgctcaaag aggatttccc cgagttcgat ttcgagattc ttgatctgtt
     1201 gcgtgacagc gggctgggtg agatgcaaga tcgtcgcggc tttagaaaaa ctccgctgtt
     1261 cggcgacgag tcgaaagctt cgcaattgac tcagttccat ggataccttt ctataaatat
     1321 aatttattaa tatgattaga tctattaatt tgaattatac tacgttctat gctaagctga
     1381 tataggaatt tattcgaacc tccaaattgg tttgggactc aaacaattag gttgatcccg
     1441 tatatacata ttggtacgaa cggaaaacaa atcacgaata atcagtcatg aattcctaat
     1501 aactaacaag gggtgagtca tcatgccgac agtggttgtg gtgggcttgc aatggggtga
     1561 cgagggtaag gggaaaatta ccgactttct ggccagtcag gcggatatgg tcgttcgcta
     1621 tcaaggcgga aataatgccg ggcatacggt agtcgtcggt gatgaggagt ttaagttgca
     1681 tctggttccc tcgggcatcc tctattccgg aactctctgc atcttgggaa atggagtggt
     1741 gttgaacccc ggagttcttc taaaggaact ggatgagtta aaaagccgcg gagttgatgt
     1801 tagcggtatc cgggtgagcg accgagccca tttgatcatg ccttaccatc ccattttcga
     1861 ccagatggaa gagaccaacc gggtggtggg aaagataggt accaccgggc ggggaatcgg
     1921 cccggcatat gtggataaat atgcgcgtta tgacgcgatc cgtgtggtgg atctgctcga
     1981 tccggagggt ttccgggagc agctcgaacg gatcgtggcc gtaaagaacg agtcgttcga
     2041 aaagatctac ggttccccgg ctctaaattg gagaactgtg tatgatgagt acctgcaata
     2101 tgccgaggcg ctccggccga tggttgccga tacctcggta ctggtaaacc aggcgattaa
     2161 aaaacggcag aaaatcctct ttgaaggagc ccagggcgcg ctgctggata tcgatcatgg
     2221 cacctatcct tttgtcagtt cctcaaatcc gacggcaggc ggcgcctgta acggtagtgg
     2281 agtggggccg accaaaatcg acaaagtggt cggggtcatc aaggcttact cgacccgggt
     2341 cggcgaggga ccgtttccct cggagctttc cgaggatttg gtttcccagc tccgggacgg
     2401 tagaggccgg gagtacggag tcaccactgg gcggccgcgc cgctgcggat ggtttgacgg
     2461 agtcatcgcc gaatattcga ccaggattaa cggggtgaat gccctgatcg tgatgaaact
     2521 ggatgtgctc gataagttgc cgaccatcca aatctgcacc ggttaccggt atcgcggcag
     2581 tatcttgaag gaatttcccg ccagcttgaa agtgctcgcg gagtgcactc cggtctatga
     2641 ggaactcccc ggatggcagc aggacaccac cggagcccgg gaactcggcg acttgccgct
     2701 caaagcccgc gagtatctca accggatctc ggatctggcc cagacgccca ttgccatggt
     2761 ttcagttggc tggcggcggg atgagacgat catgatcgaa aatatctggt aagattacgg
     2821 gaaggattta gcagttcgaa agggttgaca cagactataa aatattgtaa tatataaaag
     2881 ctgacttcaa gaattcgatg ggtcattagc tcaggcggta gagcacctga cttttaatca
     2941 gggtgtccgg agttcgagtc tccgatgacc caccattttt aaaagtttga gataagaagt
     3001 tggaagtttg aatataatat cgtaagagaa ttattttcac acttctccct ttacacttca
     3061 caattcaata cggtcccgtc gtctagaggc ctaggacacc gccctttcac ggcggtgaca
     3121 cgagttcgaa tctcgtcggg actaccaaaa atttactccg cgcaagcgga tttttttatg
     3181 ccttgccgac agtttaataa aacaccagtt aaacggcctt ttttctagtt ttctcgaggc
     3241 cctaggacac cgccctttca cggcggtgac acgagtcggc tccatgggag ccgccgaatc
     3301 tcgtcgggac tgccaaatga taatcaaacc tgctaaatgg caggtttttt gtttgcctgg
     3361 atgagcttgg tcaacgggat agaaagatac atcttttggc aggagattgt tccatatggg
     3421 ttatagcaca tcgtgaatcc ataattcata tgctggatta tattatcaag atccaagaag
     3481 ggacagagta tttcaagaat tggcagatgc aaagtggaaa caataaaagg ggctatcggg
     3541 gtaatagcgt ctcttcattt gtcttggtta acactatata aaattgggaa tacgtggtat
     3601 aataaattta aaatttcagg taaaggagga atatttgcta tgggagataa aagcccgaag
     3661 aacagtgaaa aactgaagaa aaatgccgac aagaaaacaa aaaaataacc tcaacgtctt
     3721 tgcgaagcag ttaagcaaaa agccaccgga attctactga aatgctacgg gcgtttagcc
     3781 ggtagcattt ttgactggag cgctgcttgt ggtttctgcc aattttcccc tttcatcgat
     3841 ttccaaaggc atcgaatttg ttttgacatc gtacgaacct caacatgaaa gagggaatgc
     3901 aaatggcgcg gatttttgga gatcggagag ttaatccgaa ttgggatagc cgtttaacta
     3961 ccaatcaaag ggcattacta acgacgccag ggtttggaat cgttgaaaag ttaccgtact
     4021 taccggaaga agatgttaaa aaaatcatgg acatcatcga gaaatctatc gagcaggtag
     4081 ggtcaatgat taatgcaagc aggcaataaa gcgattcact gatgatttca tatatgaacg
     4141 ccttcaaggg gcgttttatt ttttgtctgt cttaaacaac ctcaatccga aatcgattgt
     4201 tgataaagtt agtgctgata actagccggt cgaaagaaac cgggaggttt tagggattca
     4261 ggtgttggat catggggtat gcgatatatg taagtttgat aggagagagg gcagatttag
     4321 gctctttttt tgttcagagt gaaacattcg ttccgataat ctcatttctc accgaccata
     4381 tatcgtttgg ctctcacatt cgagaattag caagtaaaat tacaatcaac agaataccaa
     4441 atagaaaata tccgatgaga tattctgcaa atgtccagca atcgcccgac caaaaaatgg
     4501 acaaccggct gtctgtttca agtcgttatt atcggggccg gtccgaaaag gatagaatat
     4561 taaccttgag gggagtgatc tcaatcacta ttcctggcgc aggaattttc acgcattcac
     4621 cgagaattct tattaaactg tcactcgatc aaggagtata actgtaatgc cttacactgc
     4681 ttatattgaa gaagccggtg atgaaggatt gggcaaaggt tcccggtggt ttatcgtcgg
     4741 ggccgttatt gttaacaagt ccaaagattt ggccgtcagc cggtgcattg atgaaatcaa
     4801 acataaattc aagctgcagc cacctaatgg ccccatccat tggcgtaata tcaaacatac
     4861 aagtatccga cgcggtatca tcgaatgtct tgccggacat gagtttaata tttcttatgt
     4921 gatttgcgat acttatcatg atgaaataat gaatagtacc ttaaaaggaa gcggacgact
     4981 ttattttgat ttagccaggg ttttgattga gagaatttcc tggtttggcg ctgagcgggc
     5041 tgatacggtt gatctggttt tcgccaaccg tttcaatatg ttatatagtg atcttaaaga
     5101 ttatctcact ttgcttttaa atgatcgaac ctgccagatt aagcctggta tcatcgatct
     5161 caaccggata tcttgtcaaa atgtggggca acttaagtta ttgcaggtat cgaatatctg
     5221 taatagttca tgctttaacg cccttgaatt cgaccaatac ggctattatg acgaacggtt
     5281 tttgcttggt ctgtccaata aattttccgg cgtaaaggta atctctttag ttacggtttg
     5341 aaatttctcc ccggtaatct ttaccaatcc gaggaactgc tgcaagtcta ttcttggctt
     5401 aaaaagatat aaaaaaaaca ggccccgaga cttaaggaac ccacacgtca aagcgtgctg
     5461 ccggctatta accgaccttg agttttaccc tcgcgcatgc ctgcatttat tagtatattt
     5521 aattctactt taagtatgcc acatccttct tcatcggtca agtttcttat aatctttatg
     5581 acttaaatta aataaatttc ggaaataagg agactatatg agttttctgg ttttagaaga
     5641 tgatgagtta gatttctttt ggaattctat tcaggatatt caagtcattt ttcatcctac
     5701 ctatgccccg gaagggatgt ttaattatca agagttatcg aatcttaaaa ataaaaaaga
     5761 tgttattgtt tttcttgaca gaaacttatt gagtagcctg ttaaaattat gtgagcaagg
     5821 aaatttaacc gatgaaaaag aaaagaatac aatagccaaa gtcatgttat ggacgcttgg
     5881 aaacaacttt tccgttagcg ctggtctagc tcttaaagaa aacgcatcaa aaacgaataa
     5941 ttctgaatat gcaaaaaaag aacttcaaag attcaaagaa attattgaat actatcccat
     6001 aatgatgtgg ttgcgtcttg cagaaggagt aatggacagt aaatctccaa agccaaatca
     6061 agagttgttt tgaggaactc ctacaggagc tcccgaaacc ggtccaaaag gaaatcgtga
     6121 accaaatatt atggagtatc attggcatat tggagaggtt gctgccgagg attacatata
     6181 gacaagctat taaggacggg ggtaggttaa aggggtgggg gcggaataaa atgcttcttt
     6241 tgatttttct attttagtgg acaaaaatgt ctatgaattg acaaatgtaa aaggtagcca
     6301 tatcatactt ttaagagata aatattggtg gacattcttg tccaccaaga actttaatat
     6361 agaaagctac cagaactttt aataatgcca atttgaccac aggataaagt ttagtgtgta
     6421 tggctgtaag ttgcttgtaa gtgggattgc cctcgtgaag aatttgatga atgaggattg
     6481 ataaaatgga ccttaataat tttgaaaatt atatcgaccc caaaatagta gcaaggggct
     6541 atgactacta cgagaatgac tatgtaacat cagtggaaga aattaaggaa aatgtatttg
     6601 tggcgaaagt tgagggtact gatcgatata ctgtggaagt tgaacttgat catcaggcga
     6661 acataatcga tacccaatgt gattgtcctt atgacttagg agaatattgc aagcatcaag
     6721 cagcggtttt tcttacgtta cgggaaatga aaaacaatat atccgatgag aaaagcccgg
     6781 cgctcaaaca tcattgtatg gacttagaag cagcgtttaa atctccggtt cggaaaaaga
     6841 gaaaagagat tgatatcaag aaagtacttt ccgatcggac caaagatgag cttgttgcat
     6901 ttctactgga tattgcctct gagtatgaag aaatcaagcg acgtattgaa ctaaattttt
     6961 atattggaaa tgatgaggat gaaatcaata agtcgattgc acttatacgt acttatataa
     7021 ataataattc taacgaatgg ggatttgtgg gctatggaaa tacatctgaa gccgtcaagg
     7081 gcgccgatat ggttttggaa aaggctcgtt acgctttgga acggagtaaa accacgcatg
     7141 ctctgaaact ggccctttgt gtcgtacagg aaatgataga tttgcttcag ggggcggatg
     7201 attcggacgg cgtaattggt ggaacgattg aagaaagttt tgctttcatt gctgaaatca
     7261 ttgctgatga agaattaagt ccggtgcaga aagaagacat tttcgatcag ctgatgacgg
     7321 aagcggccaa taaacgctat gatggctgga tcgattggag gctggatctg cttgcaaagt
     7381 gttcggaact tgcggacaac cggcatttac ggaacaaatt ggagaatcgt ttggtttcaa
     7441 tgatcgagaa tgagaacgga gattcgtgga gcattaatta ttttgccgaa cagataaatc
     7501 ttatcagata ccagatgatt gaaaaatatg acggacggaa aaaggcagag gaatttatag
     7561 aacaaaactt acaatactcg agctttagaa aaaaggcaat agaaagcgca atgaagaaaa
     7621 aagattataa cgaggttgtt aagcttgcaa tcgatgggga agagaaggat aaaggttccc
     7681 ccgggctggc ggatcaatgg aagggatatc ggtataaagc atttaaacta tccggaatgc
     7741 tggatgagca gcgtagaatt gccgtggatt ttattctgaa aggagacttt gaatattacc
     7801 aggaattgaa aaacacatat gacaccaaag agtggccttc tgtttaccct gaaatcattc
     7861 ttcgattgga aaaccaaaag aaaacccatc aggatgtata tactcgtatt ctgatcgaag
     7921 aaggcgaaaa acaaaagcta tgtgagtacg ttaaaggaag gccttcaacg gtggagtgtt
     7981 tttgcaagta tcttattcct gaattcacgg aagatgttta tgggcttttt cggcaatata
     8041 ttgagcagac tgcggcgcga gcgaattcca gaaaggacta tcggggggta tgcgccataa
     8101 tccggaatct caaaaaagcc ggtgggaaac aaccggcgtt agagattaaa ca
//
LOCUS       DASVMB010000421         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_198566,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000421 DASVMB010000000
VERSION     DASVMB010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198566"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VF531_11915"
     CDS             <1..382
                     /locus_tag="VF531_11915"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEX7714710.1"
                     /translation="LVHLGYTPEEIEVAFKLLYSIPSSLKTDVQMVDDLFEIKDGYRV
                     LSQAEQRKLSLTSQGEIIRLINSSLVTSKEVEQILAEVLQSEVQEVGLRELELILHKV
                     INDDERLLMILPHPLELGPNFFLN"
     gene            648..>2652
                     /gene="topA"
                     /locus_tag="VF531_11920"
     CDS             648..>2652
                     /gene="topA"
                     /locus_tag="VF531_11920"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX7714711.1"
                     /translation="MKSLVIVESPAKAKTIEAYLGKKFTVKASVGHVADLPKSQFGVD
                     VQNGFKPKYIVIRGKGKLIKELKESAKKADTVYLATDPDREGEAISWHLANTLELDPE
                     KYKRIEFHEITKRAVENSIKNPRTIDIDRVNAQQTRRILDRIVGYELSPFLWKKVRRG
                     LSAGRVQSVAVRLICEREQEIKDFVREEFWSITGNFLTAKREPFQAKLTSFRRKKLEL
                     KDEAQTKAVEADLKNSRFIVGEVKRKERLRFPAPPFTTSSMQQEAARKLGFGARKTMV
                     VAQQLYEGLELGPEGTHGLITYMRTDSVRIAQEAQEEARETIKARFGTDFLPETPPVY
                     KTKSAGAQEAHEAIRPTSAARLPESIKGFLTRDQFRLYQLVWSRFMASQMRPAVMDTV
                     AADIVAGEYTFKANGNVVKFAGFLGIYQEGKDDETPEEEGRLPELRPEENVTLDKLEP
                     KQHFTQPPPRYTEATLIKTLEEKGIGRPSTYAPTIDTIVKRNYVLSEEKRLKPTELGQ
                     IVVDLLKQKFPEIVDYDFTAAMEEKLDEIAEGNMDWIKVLADFYDPFNREVQKANENI
                     EKVAVPVEESDIVCEKCGRKMVYKYGRFGKFLACPGYPECKNAKPIQKSLGSKCPKCG
                     VGEVLERRSKKGRHFYGCNQYPKCDFVSWERPFKEPCPQCGSMQ"
BASE COUNT          742 a          585 c          691 g          634 t
ORIGIN      
        1 cctggtgcat ttgggttata ccccggagga gatcgaggtg gccttcaaac tgctctactc
       61 gattccaagt tcattaaaga cggatgttca aatggtggat gatttatttg agatcaagga
      121 tggatatcgg gttctcagcc aggctgaaca gcgtaaacta tccttaacga gtcaggggga
      181 gatcatccga ttgatcaaca gctccttggt cacctccaaa gaagtggaac aaatactggc
      241 ggaagtgctg caatccgagg ttcaagaggt gggtttgcga gagttggagt taattttaca
      301 taaggtcatt aacgacgatg aacgtttatt gatgatactg cctcatcccc tcgagctagg
      361 acccaacttt tttctgaatt aacccaaatc gatagacaac cggactttcc cggtaatggt
      421 tggaaagaag gaggtctatc gataattttt aagctctaga tggtaaaact ttacgtatct
      481 cagattgaag tattcggtat ttaaaggtta taccgaattg aggctgtgtt tatccgcatt
      541 atttcaccgc actgaatgag catactaaat caataaatat aggatttata tggttggttt
      601 tattttggcc tggaattatc cgtttacagt aagtgggggt agttaatgtg aaatctctgg
      661 tgattgttga gtctccggcg aaagccaaaa cgatcgaggc ttatttgggt aaaaagttta
      721 cggtcaaagc ttccgtcggt catgtggcgg atttgcccaa aagtcaattc ggcgtggatg
      781 ttcagaatgg atttaagccc aagtacatcg tgatacgggg taagggcaaa cttatcaagg
      841 aattgaaaga atcggccaag aaagcggata cggtctatct ggccaccgac cctgatcgcg
      901 aaggtgaggc catctcctgg cacctggcga ataccctgga actcgatccg gagaagtaca
      961 aacggatcga atttcacgag atcaccaagc gcgcagtgga aaactcgata aaaaacccac
     1021 gaaccatcga cattgatcgg gtgaatgccc aacaaacccg gagaattttg gatcggatcg
     1081 ttggctacga gctcagccct tttttatgga aaaaagtccg tcgcgggtta agcgccggcc
     1141 gggttcaatc ggttgcggtc cgtttaatct gcgaacggga gcaggaaatt aaggattttg
     1201 tccgcgaaga attctggagt atcaccggta atttcctaac cgccaaacga gagccgttcc
     1261 aggctaaact gacctcattc cggcgaaaaa aactggaact taaagatgaa gcccaaacca
     1321 aagcagtaga agctgatcta aagaatagcc gctttattgt gggtgaggtc aaacggaaag
     1381 aacggttacg ttttccagcc ccccctttca ccaccagcag tatgcagcaa gaagcggcac
     1441 gaaaattggg gtttggcgct cgaaaaacca tggttgttgc tcaacagctt tatgagggat
     1501 tagagctcgg tcccgagggt acccacggtt tgatcaccta tatgcgtacc gattcggtcc
     1561 ggattgccca ggaggcccag gaagaggcgc gtgagacgat caaggcccgt ttcggcacgg
     1621 actttttacc cgaaacccct cctgtttata aaacgaaatc cgcaggtgcc caggaggcac
     1681 acgaagccat ccggccaacg tccgcagccc ggcttccgga atcaatcaaa ggatttttga
     1741 cccgggatca attccggctc tatcagttgg tttggagccg ctttatggcg agtcagatgc
     1801 gccccgcagt catggataca gtggctgcgg atattgtggc tggggaatac acctttaagg
     1861 ccaacggcaa tgtggtcaag tttgctggtt ttctagggat ctatcaggaa ggtaaggatg
     1921 acgaaacccc cgaagaggaa gggagacttc cggaacttcg tccggaggaa aacgtgaccc
     1981 tcgataaact ggaaccaaaa caacatttca cccaaccgcc tccccggtat accgaagcta
     2041 cgctcatcaa gactttagag gaaaaaggga tcggtcggcc cagcacctac gcgccaacga
     2101 tcgatacgat tgttaagcgt aattatgtcc tgagtgagga gaagcgtctt aagcccactg
     2161 aactcggtca aattgtagtc gatctcctga aacaaaagtt ccccgagatc gtagactatg
     2221 attttaccgc ggcgatggag gagaaattgg atgagatcgc cgaagggaac atggactgga
     2281 tcaaggtttt ggcggacttt tacgatcctt ttaaccgcga ggttcaaaag gcgaatgaaa
     2341 atatagaaaa agttgccgtt ccggttgagg agagcgatat tgtctgcgaa aaatgcggcc
     2401 gtaaaatggt ttataaatat gggcgttttg gtaaattttt ggcttgcccc ggatatccgg
     2461 agtgcaagaa cgccaaaccg attcaaaaat cactgggcag caaatgccct aaatgcggtg
     2521 ttggggaagt tctggaacgc cgttccaaaa aaggccgaca tttttacggt tgcaaccagt
     2581 atcccaaatg tgatttcgtt agctgggagc gtcctttcaa agaaccatgc ccgcaatgcg
     2641 gttcgatgca ag
//
LOCUS       DASVMB010000422         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_275875,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000422 DASVMB010000000
VERSION     DASVMB010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275875"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VF531_11925"
     CDS             <1..561
                     /locus_tag="VF531_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714712.1"
                     /translation="YFLPYVLSILIVGFVFNYIFSNLIPAVAAKLDIQFFTKSILGMP
                     DLAWLGVMFVAVWQSAALNILLYLAGLQTISEELYEASSMDGAGKWQQFRKITFPLIA
                     PFFTVNMVLSMKNFLMVFDQIMALTEGGPGRATESISLLIYRGGFLGGEFAFQSANAV
                     VYFLFILIISFVQLHYLQKREVSVSG"
     gene            554..1375
                     /locus_tag="VF531_11930"
     CDS             554..1375
                     /locus_tag="VF531_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714713.1"
                     /translation="MVKKTNWPATMVLFLGSLVILFPIYLLVVVAFKNPQEMAASIIS
                     LPQTLHWENFNQAIAMTNFFRSFKNSFTITVGAVILTLLSNSMVAYAIVRNLQRKFFK
                     FLYYYFISALFIPFPIIMLPVVKETSLLGMDNQYGLIFLYVVYGLSMNIFVYVGYLKS
                     LPGSLEEAAIIDGCSIWSLFWRIVFPLMAPIHATVGIITTLWAWNDFMLPLVMLNKPE
                     TMTLPLVQFVFRSQFTTNYNLAFASYLMAMAPVMIVYILAQKWIISGMLRGAIKS"
     gene            1391..2467
                     /locus_tag="VF531_11935"
     CDS             1391..2467
                     /locus_tag="VF531_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7714714.1"
                     /translation="MDLFQRSIEIIKENQHPGGSYVACPNFKNYQYCWLRDGSFIAYA
                     MDRSGEFDSSRRFYQWVNGVITTREGRIRKLLQKKENRQFIARDEFLPTRYRLDGQET
                     KDDWANFQLDGYGTWLWGLAEHIGATGEVELLETFQKGIGLTIAYLTVFWDTPNFDCW
                     EENGDRIHPATLACIAGGLAGINQYYQSPAITGTCKAIHEFIMKNAVHQGRLVKSIGI
                     EEVDASLLWMAVPFEIFPGDDSFMLQTVREIEAKLYKNKAGVHRYATDSFYGGGAWIL
                     LTAWLGWYYAQNSRLDEAREIKTWIENQVGEGGELPEQVPINLNQEFYYPLWVERWGN
                     PAQPLLWSHAMYLILCRELGIQRA"
     gene            complement(2514..3221)
                     /locus_tag="VF531_11940"
     CDS             complement(2514..3221)
                     /locus_tag="VF531_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7714715.1"
                     /translation="MKKLHFFTVLILSLGFSWVMAETDRSDLVKNVFHTYQSTILIRG
                     EKTIYFDPVNVAGEPHDADIVFVTHTHGDHFSIPDIQKVLKPEGTLVVPVDGAGKAKA
                     AGIKNVLTVLPNQKYEVSGLDFQTVPAYNTNKAYHPKESNWVGYICKINAVTYYMAGD
                     TDLIPEMKDIQADVAFLPIGGTYTMTAKEAAAAANLIKPAVAVPIHFADVVGTLQDAK
                     DFISLLDPSVKGVILKK"
     gene            complement(3633..>4706)
                     /locus_tag="VF531_11945"
     CDS             complement(3633..>4706)
                     /locus_tag="VF531_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX7714716.1"
                     /translation="VPADMRPDWGPDKLDTVGVIRLIKERIAPRLPKNVELGFEMHYD
                     TEVNDKTAPAIADALVDAGIHLAMITPGAHSHFGYGGIASLDPKERKGAEELGTKTVD
                     LAYGPLKKAWHADPDKAPALVLWNGSYGYDLASVGVREMYQNLKESVATLCKYEAKKG
                     GSLYIGFEPKPNEGHPAMLIPTVASALLFWRRIEEEYGVSRQKKGVNKEFGHTEMVGL
                     DLVYDTVEELDNQAMVHMHLNGQGYNDGIILGGPGKYDIDHGTRINGMNVAVAGLIDA
                     AGFNRWKGHDMLVRPYDNVEQGLDRIVRSVLSWEACAQAATEIDTKLLLKHLAARDTA
                     KAEDMMRAAVVSAQKHFDEMYKG"
BASE COUNT         1091 a         1169 c         1169 g         1277 t
ORIGIN      
        1 tatttcttgc cgtacgtact gagtatcttg atcgtcgggt ttgtttttaa ctacattttt
       61 tcgaatttga tccccgccgt agcggcaaag ctggatatcc aattctttac caaaagcatt
      121 ctgggcatgc ccgatctggc ctggctcggg gtgatgtttg tagcggtctg gcaatcggct
      181 gctttaaata tcttgttgta cctggcgggg ttacagacta tatccgaaga actctatgag
      241 gcttccagca tggatggggc cgggaaatgg cagcagttcc gtaaaatcac ctttcctttg
      301 atcgccccgt tttttacggt gaatatggta ctgtccatga aaaacttttt aatggtgttt
      361 gatcaaatca tggctctgac cgaaggcggt ccgggacggg ccaccgaatc gatctcgctt
      421 ttgatctatc gcggcggatt tctgggcgga gaattcgcct ttcaatcggc caatgcggtg
      481 gtctactttc tattcatttt gatcatctct tttgtgcaac tgcattacct gcagaaaagg
      541 gaggtgagcg tgagtggtta aaaaaacgaa ttggcctgct accatggttt tattcctggg
      601 atcattggtc atattattcc caatctatct gctggtagtg gtggcattca aaaaccctca
      661 ggaaatggcg gcatcgatta tttccctgcc gcaaacccta cattgggaga attttaacca
      721 agccatcgcc atgaccaact tttttcgcag tttcaaaaat agttttacga tcacagtcgg
      781 tgcggtgatt ctcaccttgt tgagcaactc gatggtagcc tatgccattg tccgtaatct
      841 gcagaggaaa tttttcaagt ttctgtatta ctacttcatc agtgcgctat tcatcccttt
      901 tcccatcatc atgttgccgg tcgtcaaaga gaccagttta ctggggatgg acaatcagta
      961 cggcttgatc tttttatatg tcgtctatgg gctctccatg aacatcttcg tttatgtagg
     1021 gtacttaaaa tcgctcccgg gttcgctgga ggaagcggca atcatcgacg gttgcagcat
     1081 ctggagttta ttctggcgga tcgtttttcc gttgatggct ccaatccacg ctaccgtcgg
     1141 tatcatcacg actctatggg catggaacga tttcatgctg cccttggtga tgctgaacaa
     1201 gccggagaca atgactttac cgttggtgca attcgtgttt cgctcacaat tcaccaccaa
     1261 ctataatctg gcattcgcct cgtacttaat ggccatggcc ccggtgatga tcgtttatat
     1321 cctggctcag aaatggatca tcagcggaat gcttcgcggc gcaatcaaaa gttaaagggc
     1381 aggtatttca atggacttat tccagcgcag tatcgagatt attaaggaga atcaacatcc
     1441 cggcggatca tacgtggcgt gccccaattt taaaaattat caatactgtt ggttgcggga
     1501 cggaagtttc attgcctatg ccatggatcg ctccggagaa ttcgattcgt cccggcgttt
     1561 ttatcaatgg gtgaacgggg tcatcaccac tcgcgaaggc cggatccgaa aattactcca
     1621 aaagaaagaa aatcgtcagt ttatagcgag ggacgagttt ttacccaccc ggtatcggct
     1681 ggacgggcag gaaaccaagg atgattgggc caatttccag cttgacggtt acggcacctg
     1741 gctttggggt ttggccgaac atatcggggc taccggagag gttgaactac tggaaacctt
     1801 tcaaaaagga atcggtttga ccatcgctta cctgacagtt ttctgggata cccccaattt
     1861 cgactgttgg gaggagaacg gcgatcgcat ccatcctgcg accttggcct gtattgcagg
     1921 agggttggcc gggatcaatc aatattatca aagtcccgcc ataaccggaa catgtaaggc
     1981 gattcacgaa tttatcatga aaaatgcggt tcatcaggga cggctggtga aatcaatcgg
     2041 gatagaagag gtggacgcca gtcttttatg gatggcggtt ccctttgaga tttttcctgg
     2101 agatgactcg ttcatgctgc aaaccgtcag ggagattgaa gcgaagttat ataaaaacaa
     2161 agcaggagtc catcgctatg ccacggactc cttttacggc ggcggggcct ggattttgct
     2221 caccgcctgg ttgggttggt attatgccca aaattcccgg ctggatgagg caagggagat
     2281 caaaacctgg atcgaaaacc aggtcggcga agggggagaa cttcccgaac aagtcccgat
     2341 caatctaaac caggagttct actatccctt gtgggttgaa agatggggca atcctgccca
     2401 acctcttctt tggtcccatg ccatgtattt gattctctgc cgggaactgg gaattcagcg
     2461 ggcttaaatt caggcgcagc aatgttgctg cgcctccaag gtaaatgctt ctcttatttc
     2521 ttcagaataa cccccttaac cgatggatcc aagagggaga tgaaatcctt agcatcctgc
     2581 agggttccga caacgtcggc gaaatggatc ggaaccgcca ctgccggttt gatgagattg
     2641 gccgcagcgg cggcttcttt ggccgtcatg gtataggtgc cgccaatcgg aaggaatgcc
     2701 acatccgctt ggatatcctt catttccgga atcagatcgg tatctccggc catatagtag
     2761 gtgacggcat tgatcttgca gatgtatccg acccaattgc tttccttggg atgataggct
     2821 ttgttggtgt tataggccgg aactgtctgg aaatcaagtc cggagacctc atacttctgg
     2881 ttgggaagta cggtcagcac gtttttgatc cctgcagcct tggctttgcc ggcgccatca
     2941 acaggtacta ccaaggtacc ttcgggttta agtaccttct gaatgtccgg gattgaaaaa
     3001 tggtcgccat gagtgtgggt gacgaaaacg atatccgcat catgcggctc gccggcgaca
     3061 ttcaccggat cgaaataaat ggttttttct ccccggatca gaatcgtaga ttggtaagtg
     3121 tgaaacacat tttttaccaa atcgctacgg tccgtctccg ccatcaccca agaaaatccc
     3181 agtgaaagaa tgagaaccgt gaaaaagtga agtttcttca actcataatc ccccttcatt
     3241 gaatgaattc taccaacctg ctcttgctaa attcagttta ccaattcatc ctggaaaata
     3301 gaattgcaga tctacagttc gaccttgcgg atcgtcaatt tgttctgcct atcagccaac
     3361 aatagcgctc ccggtatttc tggggggaac actggtgtcg caagtcatta tttgcataga
     3421 aatagccggt attcttttgg aatgccactc tctctaacat ttcaattttt acatatgatt
     3481 cggcaatcca tgaaaataac cttgtagata tgaaaacttt gtttcgggtt attcaagaat
     3541 agggtagcag gtatggtttc cggtcgataa aaaaaaggcc ggttgtaacc ggccctccat
     3601 ttccaccttt ataaaagtta gttccggctt cttcagcctt tgtacatctc atcaaaatgt
     3661 ttctgggcgc tgacgaccgc tgcccgcatc atatcctcgg ccttggcggt atccctggcg
     3721 gccaggtgct tcaggagcag cttggtatcg atttccgtgg ctgcctgagc gcaagcttcc
     3781 cagctcagaa cggagcgaac gatccggtcg aggccttgct cgacattgtc gtacggacgc
     3841 accagcatgt catggccttt ccaacggttg aaaccggcgg cgtcaatcaa accggcgact
     3901 gccacattca ttccgttgat ccgggtaccg tggtcgatat cgtatttgcc agggccgccc
     3961 aggatgatcc cgtcgttata tccctgacca tttagatgca tatggaccat ggcttgatta
     4021 tcgagttcct ccacggtgtc gtaaacgaga tccaacccga ccatctccgt atgtccgaac
     4081 tctttgttga cacctttttt ctggcgcgaa accccgtatt cctcctcgat tcgtctccag
     4141 aaaagcaacg ccgaggcgac cgtcggaatc agcatcgccg gatgaccttc gttgggtttc
     4201 ggctcgaatc caatgtacaa actgccgccc ttcttcgctt catacttgca aagagtggcc
     4261 acgctttcct tgaggttttg gtacatttcc cggactccca ccgaggccag gtcataaccg
     4321 taagaaccat tccagagcac caacgccggg gccttatcgg gatcggcgtg ccaggctttc
     4381 tttagcggac cgtaagccag atccactgtt ttggttccca gttcctcggc gcctttccgc
     4441 tctttgggat cgagggaggc gattccgccg taaccgaaat gactgtgcgc gccgggggta
     4501 atcatcgcaa gatggatccc cgcgtcgacc agcgcgtcgg ctatggccgg tgcggtctta
     4561 tcattcacct cggtgtcgta atgcatctcg aaacctaatt cgacattctt ggggagcctg
     4621 ggagcgatcc gctccttgat cagccggatg actccgactg tgtccagttt gtccggaccc
     4681 caatcaggcc gcatgtctgc gggcac
//
LOCUS       DASVMB010000423         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_642761,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000423 DASVMB010000000
VERSION     DASVMB010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642761"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VF531_11950"
     CDS             <1..828
                     /locus_tag="VF531_11950"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HEX7714717.1"
                     /translation="LLQIDNQKYIDDKKGDFAMKKSFVKPVFVAAMMTLFVIGFSMTS
                     YGQNFYRTIKEVLVGKYAKYIVTEPTGAPDLTIPDELKGKLYDKDGNILKQLPEKGKI
                     YNQNGEELIVSTMVSKDEKDGLVTKYEALTRKGYTERQNSKMTTMTNPEKAKPYLAFD
                     FSLPGYMPKGYAFDRIQLFNDENGIPVKNGEYAYVYFSNGDHAKDIYLQLRLMNNETA
                     YEADFGNVAEIEINGNKGVIGEGNIDVEIDGVMYMFMAGASGIDNKQLIKMAESIHR"
     gene            complement(1056..1448)
                     /locus_tag="VF531_11955"
     CDS             complement(1056..1448)
                     /locus_tag="VF531_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7714718.1"
                     /translation="MKQVYDFLKKCRTYYIATIDGDQPRVRPFGTIVLYEDKLYIQTG
                     KVKNVSQQMQANPKIEICAVDGGEWIRVAATAIRDDRVQAKQHMLEAYPELQGMYKAD
                     DNNTEVLYLKDATATIYSFTAEPRVIRF"
     gene            complement(1749..2435)
                     /locus_tag="VF531_11960"
     CDS             complement(1749..2435)
                     /locus_tag="VF531_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714719.1"
                     /translation="MKKILIIEDDLNIAELERDYLKLNGFAADIEADGVKAAKMAVSG
                     DYEVIIVDLMLPNKNGYEIIKEVRKSLEIPVIVVSAKTEDIDKIRGLDYGADDYLTKP
                     FSPAELAARVKSHINRYERLRGNSTALEVIAHKGLEINTASHKVLVNGREVQLTSKEY
                     ELLVFLASNPNVVFSKEHLFNSVWGDEDFGDIATVPVHIQKIRKKIEKDPANPELIET
                     LWGTGYRFNA"
     gene            complement(2446..>3730)
                     /locus_tag="VF531_11965"
     CDS             complement(2446..>3730)
                     /locus_tag="VF531_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7714720.1"
                     /translation="HKKRLKRHWQLHKFQRELRGQLDELYRRRRRIQGLLRENRNLNR
                     QELQDELERIEKQVQVLHLRMHLKNGNFNEYYRHIRYTRPVALIFNLTLWFLLFRFGG
                     ISTGLKIVILFMALMTTAGSIGEAIFLVRIRERILKPVENLRRGVEEIAQGNYQVEVA
                     NDFSNEMGTLTVAFNRMARKLKEDEELKLEYEANRKTLIANISHDLKTPITSVQGYLE
                     ALMERNDFSAEKNEKYLKIIYNNTVYLNKLIDDLFLFSKLDLDKLDFNFEPISIRPFF
                     NDLLEEFRLDLEEKKVAFAYRDELDDEYTLKMDAKRIHQVVRNLIDNAIKYGSAKDLA
                     IQARLYSKSGFVYLDICDNGPGISQEELKHVFERFYRAAAERPKDLNSTGLGLAIAKE
                     LVEAHGGKIIASSSERGTCFTLMLPVPVETAGENE"
BASE COUNT          981 a          912 c          773 g         1064 t
ORIGIN      
        1 ctattacaaa ttgataatca aaaatatata gatgataaga aaggggattt tgccatgaaa
       61 aagtcatttg taaaaccggt ttttgtagct gcaatgatga ctttattcgt tataggcttt
      121 tcaatgactt cttacggtca aaatttctat aggacgatta aagaagtatt ggtcggaaag
      181 tatgccaaat atatcgtgac ggaaccaacc ggcgcgccgg atttaacgat tcccgatgaa
      241 ctgaaaggca aattatatga caaagacggg aatatactta aacaacttcc tgaaaaagga
      301 aagatttaca accaaaacgg cgaggaattg atcgtctcga cgatggtttc caaggatgaa
      361 aaagatggtc ttgtcacaaa atatgaagcg ctgacccgaa aaggatatac cgagcgacaa
      421 aacagcaaaa tgactacaat gaccaatccg gaaaaagcaa agccctacct ggcatttgac
      481 ttttcattac ccggctatat gccgaagggc tatgcatttg acagaatcca actgttcaat
      541 gatgaaaacg gaataccggt taaaaacggc gaatatgcct atgtttattt cagcaacggc
      601 gaccacgcca aggatatcta tttacagctc aggttgatga ataacgagac tgcctatgaa
      661 gcagatttcg gcaatgtcgc agagattgag attaacggta ataagggagt tatcggcgaa
      721 ggcaatatcg atgtggaaat tgacggtgtt atgtacatgt ttatggccgg ggcttccgga
      781 attgataata agcaactgat taaaatggct gagtcgattc atcgataatg ttaattgaac
      841 cgtgaccttt gcctttacac tggatctgat ttaagtacaa aaaaaagacc acattccgaa
      901 agttatattt ggatgtggtc ttttcctttg gtaaaataac ccacctcaaa aaacgtcgtc
      961 caaccacctt cgagagtttg attgaaaata tttcagctcc gaacaaccgc ttcaggaccc
     1021 aatcttgttt tctttggctt tactccgaaa taactctaaa accggattac acgaggttca
     1081 gccgtgaatg aataaatcgt ggccgtggca tcttttagat acaacacttc cgtattatta
     1141 tcatctgctt tatacattcc ctgcaattcc ggataagctt ccaacatgtg ctgcttcgct
     1201 tgcaccctgt cgtcccttat agcggttgcc gcaacccgaa tccactcacc gccatccacg
     1261 gcgcagattt ctattttggg atttgcctgc atctgctggg aaacattctt gactttgcct
     1321 gtttggatat aaagtttatc ctcatataaa actatggttc cgaaaggacg cacccttggc
     1381 tgatcaccgt caatggttgc aatgtaatat gtccggcact tctttaaaaa atcatatact
     1441 tgtttcattg aaccaacctc ccacctgctt tttgcttggt ctttggtaat tatttttatt
     1501 ttaccgctac cattttattt caacttttct tcgatcaaac atactccgga taaccccggt
     1561 tttcctgctc gtacattttt tcttttgtat attctgtttc ttctgttctc ctcctaaatg
     1621 tcaaaattct acagaactct ctcgtaaacc atagaattaa aaaagcgcca aaagattttt
     1681 actatggcgc tccgattacc atccattttc aaatcttgta cactttattc accggaaaaa
     1741 actaatcctc acgcattaaa ccgatatccc gtcccccaga gcgtctcaat tagctccggg
     1801 ttggccgggt ccttctcgat ctttttgcgg atcttctgaa tatgcaccgg cacggtggca
     1861 atatccccga aatcttcatc gccccatacg gagttgaaca gatgctcctt cgagaaaacc
     1921 acattgggat tggaggctaa aaaaaccaat aactcatact ccttggaggt gagctgcacc
     1981 tcgcggccgt tcaccaaaac cttgtgcgag gcggtgttga tctccagtcc cttgtgggca
     2041 atgacttcga gcgccgtgct gttgccccgg agcctttcat agcggttgat gtgggatttg
     2101 acccgggccg caagttcggc ggggctgaag ggtttggtga gataatcgtc ggccccgtaa
     2161 tccaggccgc ggatcttatc gatatcctcg gtcttggccg aaaccacgat caccggaatt
     2221 tccagactct tgcggacctc tttgatgatc tcgtacccgt ttttattggg cagcataaga
     2281 tccacgatga tcacttcata atcgccggac acggccattt tagcggcttt taccccatcg
     2341 gcctcgatgt ccgcagcaaa cccgttcagt tttaagtaat ccctttcaag ctccgcgata
     2401 ttcaggtcgt cttcaataat caggatcttt ttcattctgc gcctctcact cgttttcccc
     2461 ggccgtttcc accggaaccg gaagcatcag cgtaaaacat gttccccgct ccgaactgga
     2521 tgcgataatt ttccccccat gggcctcgac cagttccttg gcaatggcta aacccagccc
     2581 ggtgctgttg agatctttgg ggcgctcggc ggcggcccgg taaaaccttt cgaaaacatg
     2641 cttcagttct tcctgtgata ttcccggccc attgtcgcaa atatccaggt atacgaaacc
     2701 gctcttgcta tatagtctag cttggatggc caaatccttt gcggaaccat acttgatcgc
     2761 attgtcgatc agatttcgca ccacctgatg aatccgcttg gcgtccattt ttagcgtata
     2821 ttcgtcatca agttcatccc ggtacgcaaa cgccactttc ttttcttcga gatcgagccg
     2881 gaactcttcc aaaaggtcgt taaagaaagg ccggatgctg atgggttcaa aattaaaatc
     2941 gagcttatcc agatcgagct tggaaaagag aaacaagtca tcgattaatt tattaaggta
     3001 cacagtattg ttatagatta tttttaggta tttctcattc ttttcagcgg agaaatcgtt
     3061 cctttccatc agcgcctcaa gataaccctg caccgaggtg atgggagtct tgagatcgtg
     3121 cgagatattg gcaatcaacg tcttacggtt cgcctcatac tccagtttca gttcctcgtc
     3181 ctccttgagc ttgcgggcca tcctgttgaa agcgacggtc agtgtgccca tctcattcga
     3241 aaaatcattg gccacctcga cctgataatt gccttgggcg atctcctcta cgccccggcg
     3301 cagattttca acgggtttca ggatccgttc cctgattctg acgagaaaaa tggcttctcc
     3361 aatactgccg gccgtggtca tcagcgccat gaataaaatg acgattttca atcctgtgct
     3421 aattccgccg aacctgaaaa gcagaaacca cagcgtgagg ttgaaaatga gcgccacagg
     3481 ccgtgtatac cggatatgac gatagtattc gttaaaattc ccattcttca ggtgcatccg
     3541 cagatgcaaa acctgcactt gtttttctat ccgctcaagc tcatcctgta attcctggcg
     3601 attaaggttc cggttctcgc gtaataaacc ttggatcctt cgtcgcctgc ggtatagctc
     3661 atcgagttgg ccccgtagtt ccctttgaaa cttatgcagc tgccaatgac gtttcaatcg
     3721 tttcttatga
//
LOCUS       DASVMB010000424         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_716043,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000424 DASVMB010000000
VERSION     DASVMB010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716043"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF531_11970"
     CDS             complement(<1..326)
                     /locus_tag="VF531_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714721.1"
                     /translation="MNRTIQKYLNLEFFSVIYDLLEAYLAILFGSMAHSIALIAFGFD
                     SIMESLAGLVLISWLSLKKELTSEAAYRRKRLIMRTVAWAFLILGAILLYVTVKRLVV
                     GEPARPS"
     gene            687..1115
                     /locus_tag="VF531_11975"
     CDS             687..1115
                     /locus_tag="VF531_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7714722.1"
                     /translation="MKATHLGMTVRDTGKTLDFYCNVLGAKLLWEPGPSQQGSQTDTI
                     FGLANTRVLVSGIDLHGMVIEFFQFLEPRVQEDSFKGDYRIGGWKHLALEVEDIETEV
                     EKLKQKGVYFRFPIQTLSNGCRMTYFEDPDGIMLEFIQQP"
     gene            complement(1235..>1633)
                     /locus_tag="VF531_11980"
     CDS             complement(1235..>1633)
                     /locus_tag="VF531_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714723.1"
                     /translation="EAICLLDETYMQLMNYKGFFYQAANGTKLQSLPNMAIRLDPENS
                     RSYIDLAMFYINAPGFAGGSIDKAIERLKKAAQSEDKFDQFDVNLWMAIAYQKKKDMD
                     TAAHYIKRALQIYPGNSWAQSILNDLDSSK"
BASE COUNT          450 a          389 c          379 g          415 t
ORIGIN      
        1 gaaggccggg ccggctcccc aaccaccagg cgtttcacgg tcacatagag caggatggct
       61 cccaaaatca aaaaagccca agccacggta cgcatgatca gccgcttacg gcggtacgcc
      121 gcttcactgg ttaactcctt ctttaggctc aaccagctga tcaagaccaa ccctgctaag
      181 gattccatga tgctgtcaaa cccgaaggca atgagcgcga tgctatgggc catactccca
      241 aaaagaatcg ccaggtacgc ctcgagtaaa tcgtagatca ccgaaaagaa ctccaggttc
      301 aaatatttct gaatggtgcg attcatgaat aaacctccaa aacttcacgc gcttagacgc
      361 gcaataaagg cggtatttaa atagtctatc tgatttgcga aaatatattt tcgctttgcg
      421 caaggagatt cctctcgttt cggaccaatt attgcgaaaa ataaatccgt ctcaatgaaa
      481 acgccgtgtt gatgctgact ttaccggctc cgttgtgata aaatgtttcc gggcaatcaa
      541 ttcggaatac ataaaaaaga cgcatcgatg tcgaaaacaa ctgccaataa agcagtcttc
      601 atcctgcgtg ctaccgtcgg ggagcgatac aggatctatg ccctgcattc ggggagaatg
      661 aataagaata aaaaggaggg tacgcgatga aagccactca tttgggaatg acggtacggg
      721 ataccgggaa aactcttgat ttctactgta atgttcttgg agccaaatta ctgtgggaac
      781 cggggccgtc tcaacaaggc agccagaccg atacgatctt tggtttggca aacacccggg
      841 ttttggtctc cggcattgat ctgcacggca tggtaatcga gttttttcaa tttctggagc
      901 cccgggttca ggaggactcg tttaagggag attaccggat cggagggtgg aaacacctgg
      961 cgctggaggt cgaggatatc gagacggaag ttgaaaaact caaacaaaaa ggagtctatt
     1021 tccgtttccc cattcaaacc ctgtccaacg gttgcaggat gacgtacttt gaagaccccg
     1081 acggtatcat gctggaattc attcaacaac cttaagtaca ttactcatac gatcaaactg
     1141 ccgccagatg gcggcagttt ggcattaagt acagatcgtc cggcgcagtt cgttccggta
     1201 aatgtctgcg aatctagatt caaacatccc ggatttattt agatgagtcc agatcattta
     1261 aaatgctttg agcccaagaa ttcccggggt agatttgcaa cgcccgcttg atataatggg
     1321 cggcggtatc catgtccttc tttttttggt atgctatcgc catccaaagg ttgacatcga
     1381 attggtcgaa tttgtcctca ctttgagctg ccttcttcag cctttcaatt gctttgtcaa
     1441 tacttcctcc ggcaaatccg ggcgcattga tataaaacat tgccaaatcg atataggatc
     1501 gactattttc cggatcaagc ctgatggcca tattgggtaa cgactgcaat tttgtgccgt
     1561 tggccgcctg ataaaaaaaa cctttgtagt tcatcaattg catatacgtc tcatcgagca
     1621 gacagatcgc ttc
//
LOCUS       DASVMB010000425         6479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_493881,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000425 DASVMB010000000
VERSION     DASVMB010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493881"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1379)
                     /locus_tag="VF531_11985"
     CDS             complement(162..1379)
                     /locus_tag="VF531_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714724.1"
                     /translation="MDTDVMKFLKQHYIGLGMVFIIVFWACFLGMLNFPPVYRVNAAI
                     EVDRQGTDIENIDQSYDQNVVQVNNTVQKLKSATLLRQTLLKLGYDYPIESREFMALR
                     DNLNVKPLQPSNVIQITFKHRSALEAAQIVNNLVDNLMAENQDSRKAKIDEAISYVTS
                     SLSNIRGQLGPQNLSVKAQRYNPQRIGDLLRETENELNRVNRYLAQFKATLTPTERKA
                     VENVLTPQLTEKFKSSLASLESNLIADLQDKNWKHPVIVKDQQIIWREKTRLTASLKT
                     ILEAQSYGSRIQTSDDLCTYQVKSFELQLRRDELQNMSNRNLTRPSDTQATVLKDLEE
                     RLVQKLVELENLNDPTDIRITWIDKAVPPSDPYFPPGVITIIGLSLGAAVLGSLIVMV
                     LLLEVKSTRQTKV"
     gene            2026..3378
                     /locus_tag="VF531_11990"
     CDS             2026..3378
                     /locus_tag="VF531_11990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7714725.1"
                     /translation="MNHNNNIGFLLTRLAGDILLINGAILLAFGIRFLFVVPEYNFRS
                     YQILWPIISILGPLLFGFFGLYRTENRSWTEVMASIIVSLIFLTLILIATSYMIGVHS
                     FPRSVFATALLLQMIILGIWRWFMLQWEAHLAPMIRILIIAPLAEAGEIFTRLVHDNY
                     FVIGVIVERLTGKENVTPFKLLGEYRDIPSAFDGGRPDTVVISSHLPEAVKNLVALNA
                     MRFNIPVVFNPGPYEIMVSQSKIEQIQDTMSFVAYSKDYPGREQLKRILDLGISLGSL
                     ILCAPFMILIIISIKLSSPGPIFFKQERIGLKGRRFQLYKFRTMIPDAERETGPILAS
                     SNDPRVTQVGKLLRAMRLDELPQLFNVIKGDMSIVGPRPERPYFVKLYETQLPQYHLR
                     HLIKPGITGLAQISGRYSMSAKDKLQYDLLYMKNATILLDLKIILQTIKVVLIPEKAS
                     "
     gene            3624..>6479
                     /locus_tag="VF531_11995"
     CDS             3624..>6479
                     /locus_tag="VF531_11995"
                     /inference="COORDINATES: protein motif:HMM:NF037694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX7714726.1"
                     /translation="MHSNQDPVLPGGEGGRYRLIHRLLWLTFVLALVISLYIPSFAAN
                     SVRIIRMEPSGEVSPKTNFVFTFSADVVTKPRIGKLMSNNRIRFKPEVPGKIRWESSR
                     RLKFLPEAPLQPSTAYTVEFKPDFLEELKKNLTGERKFKFTTERFRITGSNLDFIYNP
                     ARKRGIIFQARLSFNYPVAIENLERQLSLVFVDKRQPIKFSITQQNGGRDALITSELL
                     IRGTKARKVELSLPQNFLCTGAKIGLKDKFVKGADFREEQNLNINEINAQSEDGQNVI
                     TIRCSEPVDPDVAVNFISLKPKTEFRISTSGEQITLSGSTLKPGQTYLVKINKGLPAL
                     NGKPLEREFSDEVTFPDLEPSVSFSAPGRYLASKGYRNLGMETVNISKVEVEVAKVFA
                     NNIVSFLSSLNDEGYCYSSDLERYGRVIQTLTYETSGGENDIITTPVNLSRYLDGQRG
                     IFQIYACDPENRWRSTAKIAVITDLGIMAKMGGDDLVVWVNSLDNLTPKSGVKVSLVS
                     FNNQEIVSGTTDADGKVRFSGLRQQMENFRSYVILAEQADDLSFVCLDDSLIDKTDFN
                     VAGRPQTQGYEAFVYTDRGVFRPGDDANIVAIIRGANAAVPPEFPVRLEVIGPDGLVF
                     REFAATTKEDGICQFHLAIPDYARTGKYTALMYVAKQSIGSTAFNVEDFIPDRIKVET
                     LTNKDRFTTGESAKITVRGTNLFGPPATGRRVEVGVRLEPVIFSPAAYRSYTFGDSGR
                     EFRTIEEDLGQTQLDADGQASFDYNFPDNLYPPGYIRAVFQATVTEDGGRSVSNYKAA
                     EFHPYKSYIGYKTKNSSYAKIGETYPISLVEVSPDGKPFTNKTLVVDVFSVTWNSIYR
                     RDRDGEYSYSSERQETRISLDSVKLTDGEGEFNFRPKDYGYFKIVFADPNTGARGVYE
                     LYASGWGYAPWAMDHPDRVQLDLDQSTYKTGQNATLQI"
BASE COUNT         1640 a         1664 c         1488 g         1687 t
ORIGIN      
        1 tcccatgacg aagggttttt ttgcaccttt cacgccatag ccattggagg aattccgata
       61 accgtctcac catcttcccc ccccgttcaa agcaccgaac gttccgatag ggttactgcg
      121 gctggctatt tcccccatga tgagcatttg gattactaat ctcacacctt tgtttgtctg
      181 gtactcttca cctcaagtaa caacaccatg acgatgagac tccccaatac cgctgctccc
      241 agactcagcc cgataattgt gatcacgccc ggaggaaaat aggggtctga cggcggcacc
      301 gctttatcaa tccaagtgat ccgaatatct gtggggtcgt tcagattttc gagctcaacc
      361 agcttttgaa cgagccgttc ttccaaatcc tttaataccg tcgcttgggt gtcactcgga
      421 cgagtaaggt tccggttcga catattttga agttcatccc ggcgaagctg cagctcaaaa
      481 cttttaacct gataagtaca gagatcatct gatgtttgaa ttcgggaacc gtacgattgt
      541 gcttccaaaa tcgtcttaag ggaagcggtg agccgtgttt tctccctcca gatgatctgc
      601 tgatccttta caatcacggg atgcttccaa ttcttgtctt gtaaatccgc gatcaggttt
      661 gattccaggg acgccagact ggatttgaac ttctcggtaa gttgaggagt aaggacgttt
      721 tcaacggcct tacgctcagt aggggtcaac gtagccttga actgggccag atatcggtta
      781 acacgattga gttcattctc agtctccctg agcagatccc cgatccgttg aggattgtag
      841 cgttgagcct ttaccgaaag gttttgtggc cccagctgac cccggatatt ggacaggctg
      901 gatgtaacat agctgatggc ctcatcaatc ttggcttttc gggagtcttg gttctccgcc
      961 atcaaattat ctaccagatt attgacaatt tgggctgctt caagggctga acggtgtttg
     1021 aaggtgatct gaatcacatt gctgggttgc agcggcttaa cgtttaaatt atcgcgtaac
     1081 gccataaact cccgggattc gatagggtaa tcgtaaccca gtttaaggag agtctgccga
     1141 agcaaggtgg cactcttcag tttctgtacg gtattgttaa cctgaaccac gttttgatca
     1201 taggattggt cgatattctc tatgtctgta ccttgccggt ccacctcgat cgccgcgttc
     1261 acccggtaaa ccggaggaaa attaagcatc cccaaaaaac aggcccaaaa aacaatgata
     1321 aacaccattc ccaatcctat ataatgctgt ttgaggaatt tcattacatc agtatccacg
     1381 cagccaactc ctttaaaaga ataaatagtt caaacgtttt atcgtaggat cattgtcatt
     1441 tggaaactga ggaactcagg atacgaatca taattcttat cggcagattc cttgaagatt
     1501 tttaggaaat cagatcactt tactttaatt accagggata tttcaattgt tggctcacca
     1561 atgtcaggtg taccaagtga ttgcagtacc tgactcatac caagaaaatg taaaaaactc
     1621 tattaactat cggcggctaa aaaccgatga tttgattaat aggctatagt catccaaatg
     1681 ctttcacaaa atgatggatc atccttatca tcgggtatac agccattcgg gacatgatgt
     1741 agttgacgat tcactgtaac aatcaaaaaa ccgcgggttt cctcgactga ccggagatac
     1801 catctttgcc caagaattgg ccgaatgttc ctaccctatt tttaaccgtc tttattccca
     1861 caaaatttcg ttattagtcg tcctctcctt tgttttggca tcattatcat tttactgcga
     1921 caaaaatctt ccggaaatat tgaatatata tgatggaaca gttcagcacc cagttgactg
     1981 taacaatatt ccggatgaag gcaccggata gcggaggcaa tacagatgaa tcataataat
     2041 aacatcgggt ttcttctgac ccggttggcc ggagatatct tactcataaa cggggctatt
     2101 ctcctggcat ttgggatccg gttcctattc gtggtaccag agtacaactt ccgttcctat
     2161 cagatccttt ggccaataat cagcattctt ggaccgcttc tctttggatt ttttggtctg
     2221 tatcggacag agaaccgtag ctggactgag gttatggcct caatcatcgt atccctcatc
     2281 tttttgacgt tgatcctgat cgccaccagt tatatgatag gtgttcacag ctttccaaga
     2341 agtgtgttcg ccactgcctt gttactccag atgatcatcc ttggcatatg gcgttggttt
     2401 atgctccaat gggaagccca cctggctccc atgatacgaa tccttatcat cgcccccctg
     2461 gccgaagccg gggagatctt caccagattg gttcatgaca attattttgt catcggagtg
     2521 atagtagaac gcctgaccgg gaaagaaaac gttaccccgt ttaagcttct gggcgagtac
     2581 cgggatatcc cttcagcgtt tgacggaggc cggccggata ccgtcgtcat ctccagtcat
     2641 ctacccgaag ccgtaaaaaa tctggtagcc ctaaatgcca tgcgtttcaa catcccggtt
     2701 gtttttaacc ccgggcccta tgagatcatg gtctcccaat ccaagatcga acagattcag
     2761 gatacgatga gctttgttgc ctactccaag gattatcccg gccgcgaaca actcaagcga
     2821 atcctcgatc tgggaatttc cttaggcagt ttaatactct gtgccccatt tatgatactt
     2881 atcattatct cgatcaaact cagttccccg gggccgatct ttttcaagca agaacggatt
     2941 ggcctcaaag gaagacggtt ccagttgtat aaatttcgaa ccatgatccc ggacgccgaa
     3001 agagaaaccg gaccgatact cgcatcaagc aacgacccgc gtgtcacgca ggttggcaaa
     3061 ttgttgcgtg ccatgaggct ggacgaattg ccccagttat tcaacgttat caaaggcgat
     3121 atgagtatcg tgggtccccg tcccgaacgg ccgtattttg taaaattata tgagacccaa
     3181 ttaccccagt atcatctacg tcacctcatc aaaccgggga ttaccggtct ggctcagatc
     3241 tccggaagat acagtatgtc cgcaaaggat aaactccagt atgacctctt gtatatgaaa
     3301 aacgccacca tattattgga tctaaagatc attttgcaaa ccatcaaggt cgttttgatc
     3361 cccgaaaaag cttcctaatc ccccactatc atctaacaaa cagtcaatgc ttcacaatct
     3421 ccccgcctgg ggaggattgt ttcacccgcc tttatccctt cattccgtgg tggactttta
     3481 ccatcgtaaa caactgtccc ggaaaattga ttttacagtg caggaaaata atgtatgaca
     3541 gcgaattggt ttgatcacac catataaatt tgctaaaaaa gtaacggttt ggatcgattc
     3601 aagtgatgag gagttgattg gggatgcatt caaatcagga tcccgtttta ccgggcggtg
     3661 aaggcggcag ataccgcttg attcaccgcc tcttatggct gaccttcgta ttggcgttgg
     3721 tcatttcttt atatattccc tcctttgccg caaactcggt ccggatcatt cggatggagc
     3781 cctccggtga agtaagtccc aagacgaatt ttgtctttac cttctctgcg gatgtggtga
     3841 cgaagccgcg gatcggtaaa ttgatgagta ataatcgaat ccgtttcaaa ccggaggttc
     3901 ccggcaaaat tcgttgggaa tcttcgcgcc gccttaaatt cctgcccgaa gccccattac
     3961 aaccctccac cgcctataca gtcgagttca aacccgattt cctggaagag ttaaagaaaa
     4021 acctgaccgg cgaacgtaag ttcaagttca ccaccgaacg tttccgcatt actggaagta
     4081 acctcgattt catttataat ccagcccgga aacgcggcat catctttcaa gcccgtctga
     4141 gtttcaacta cccggtggcg attgaaaacc ttgaacgcca gctctccctg gtctttgtgg
     4201 ataagcgcca gccaatcaag ttttctatca cacaacaaaa cggcggccgc gatgccctca
     4261 tcaccagcga attgttgatc cgcgggacga aagcccgtaa ggtagaactg agcctgcccc
     4321 agaatttcct atgcaccggg gccaagatcg gtctcaagga taaattcgtc aagggcgccg
     4381 atttccggga ggaacaaaac ttaaatatca atgagattaa cgcccaatcg gaagacggcc
     4441 aaaatgtcat cacgattcgc tgttccgaac cggtggatcc cgatgtcgcc gttaacttca
     4501 tctccctcaa gcccaagacc gagtttcgca ttagtaccag tggcgagcag atcactctct
     4561 ccggcagtac cctgaaaccg ggtcagacct atctggtcaa aatcaacaaa ggtctgcccg
     4621 ccttgaacgg taaacctttg gaaagggagt tttcggatga ggtaaccttc cccgacctgg
     4681 agcccagcgt cagctttagc gcacccggac gttatctggc aagtaaaggc tatcgcaacc
     4741 tgggaatgga aacggtcaac atttccaagg tggaagtaga ggtagccaaa gtatttgcca
     4801 ataacatcgt ttccttcttg agcagcctta acgatgaggg ttattgttat tcgtcggatc
     4861 tggaacggta cggaagggtc atccaaaccc tcacttacga gacatccggc ggagagaacg
     4921 acatcatcac caccccggtt aatttaagcc gctatctcga tgggcaacgg ggaatcttcc
     4981 aaatttacgc ctgcgatccg gagaaccgtt ggcggagtac agccaagatc gccgtcatta
     5041 ccgacctggg catcatggcc aaaatgggcg gggacgacct ggtggtttgg gtcaactccc
     5101 ttgacaattt gacgccaaag tccggggtca aagtcagtct cgtcagcttc aataatcagg
     5161 agatcgtcag cggtaccacc gatgccgacg gcaaggtgcg tttcagcggt ctccggcaac
     5221 aaatggagaa tttccgttct tatgtcattc tggcggaaca agcggatgat ctctcctttg
     5281 tctgcctgga cgacagcttg attgacaaga ccgattttaa tgtggccggc aggccccaga
     5341 cccaagggta tgaggccttc gtctataccg atcgcggcgt gttccggccc ggtgacgacg
     5401 ccaacatcgt cgccatcatc cggggcgcaa atgccgccgt accgcccgag ttcccggtgc
     5461 ggctggaagt tatcggtccc gacggcttag tctttcgtga atttgccgcc actaccaagg
     5521 aggacgggat ctgccaattc cacctggcta tccccgacta tgcccgcacc ggtaaatata
     5581 cggccctgat gtatgtggca aagcaatcca tcggtagtac agcctttaat gttgaagact
     5641 tcattcccga ccggatcaag gttgagactc tcaccaataa agaccggttc acgacgggtg
     5701 aatctgcaaa gatcaccgtc cgtggtacaa atctgttcgg tccccctgct accggccgcc
     5761 gtgtggaagt tggagtccgc ctggaaccgg tgatttttag ccccgccgct taccgttctt
     5821 atactttcgg tgactccggg cgggaatttc gaaccatcga ggaagatttg ggccaaactc
     5881 agctggatgc ggatggccag gcatcttttg actacaactt tcctgataat ctctatcccc
     5941 cggggtacat ccgcgcagtt tttcaggcta cggttactga agacggcggc cggtcggtga
     6001 gtaattataa agcggccgaa ttccatcctt ataagtcgta tattggttat aaaaccaaga
     6061 acagctctta tgccaaaatc ggggaaacct atcccatttc tctggtggaa gtgagccccg
     6121 atggtaagcc ctttaccaac aaaacgttgg tggtcgatgt cttctcagtt acctggaatt
     6181 ccatctaccg ccgggatcgg gatggggaat actcttactc cagcgaacgt caggaaaccc
     6241 gaatcagtct cgacagtgtc aaacttaccg acggtgaagg cgaatttaat ttccggccta
     6301 aagactacgg ttactttaag attgttttcg ccgaccctaa caccggggca cggggcgttt
     6361 atgagcttta tgccagcggt tggggttatg ctccctgggc gatggaccac cccgaccggg
     6421 tccagcttga tctggaccaa tccacttaca aaaccgggca aaacgccact ctgcagatt
//
LOCUS       DASVMB010000426         7756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_643115,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000426 DASVMB010000000
VERSION     DASVMB010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643115"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VF531_12000"
     CDS             <1..427
                     /locus_tag="VF531_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HEX7714727.1"
                     /translation="IKYGKPVGATPNGRHAGTPISFGANPDPGFRKDSAPTAMVKAVA
                     RVQSGYGNTVPIQLELDPGLSREEGGIEKVADLIETHFKLGGTLFNINVIDAEKIREA
                     HRDPTKYPDLVVRVTGFTAYFAALSPEFRQLVVDRIIQE"
     gene            645..1547
                     /locus_tag="VF531_12005"
     CDS             645..1547
                     /locus_tag="VF531_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX7714728.1"
                     /translation="MNIEVSVKNMNFLEAFASETRVKIIELLNEKPLNVKEIAAYLDI
                     SSAIVTRHIQKLEQAGIIKCENSAGKRGTQKVCSLKLEQATLQFKFKVSEANANTYCC
                     SIPVGQYSSYDIKPTCGLASTGGVIGVVDDPRYFADPDHIQAGIIWFGQGFIEYRIPN
                     YLLSNQRAKFVQITLEICSEAPGYNEDWPSEITFYLAGRELGFWTSPGDFGSSKGIFT
                     PDWWNLGTQYGLMKTIIINETGSYVDGIQISTVTVNDLDIRYGKEILFRMASLVKSKN
                     SGGVTLFGKGFGNYSQDIQVKIGY"
     gene            1575..2798
                     /locus_tag="VF531_12010"
     CDS             1575..2798
                     /locus_tag="VF531_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7714729.1"
                     /translation="MANHIPENDTDKVIKVNKEISIPLYSQIKTIMLQSIASGEYKLG
                     EKIPSEIELAKKYGVSRITTKQALNDLMQEGIVDRVAGKGTFVRRAAPLRNDTADSPV
                     NPQPLRVRGGQPGSFGLIVPFMKDTYEIGIISGVEQELRDRDYRLIFRKTDNSIIEES
                     KAIESLRNEEVQGLIIFPAEGMYCSEALLKLKLEQFPFVMVDRFYRGIECNYVVSDNF
                     GGAYAAGEYLIHLGHKEFGLIGYQPELAVSINDRIQGFCQALADQGIPLSQGRKELSM
                     IREQTYDRHTGQADPVAIQTVQAFLERNPRITAIFAINDYLAICVLRAALQMGLNVPR
                     DLSVMGFDNNILAANVEVPLTTVNQPKEEIGKSAAQLLQGMIETENPVMEHLVLSTEL
                     IIRASTAPPGESKRV"
     gene            complement(2795..3634)
                     /locus_tag="VF531_12015"
     CDS             complement(2795..3634)
                     /locus_tag="VF531_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7714730.1"
                     /translation="MFRFGVRAHDFGKLPVEELAKRISDRGIPSIQLALGKAVAGLDA
                     GPGSFSPGLARYIGDTFANHNIQIAVLGCYNNLIHPDPGIRRDYIARFKEHLRYARDF
                     GCSVVGTETGSLNGDYSFHPDNHGETALRLFLESLTELVTEAEKFGVFVGIEGVASHV
                     VSSPDRMKQVCDTIQSNNLQVILDPVNLLTVENYEDQDRILDQAFEQYGKRIVVLHLK
                     DFTIEHGQKNSAPIGDGLFHFEYLLKKVQSRKPYINMLLEETTPETVAASIQKLQEIA
                     GRV"
     gene            complement(3708..5759)
                     /locus_tag="VF531_12020"
     CDS             complement(3708..5759)
                     /locus_tag="VF531_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7714731.1"
                     /translation="MINSKLPSIYFGGDYNPDQWPEEVWQEDMRLFKLAGINVITVPV
                     FSWAKLQPAEDRYDFEWLDRVLDLAAKNGIYVCLATSTAAQPAWMSLKYPEILPVDIQ
                     GRKKTHGGRVNFCPNSQVYREFSTKLASHLALRYKDHPALLVWHIANEYGGNYCYCEH
                     CAAEFRNWLKKRYGTIEELNKRWNMSFWGHTVYEWDEIVPPSYLNEMGMATWRVPPAE
                     FTCFQGISLDYNRFMSESILECYLGEYQAIKAITPNIPITTNLMGTYKPLDYFDWAKH
                     MDIVSWDNYPSLNALPANIALRHDLMRGLKDGAPFMLMEQTPSQQNWQAYNSLKRPGV
                     MRLWSYQAIAHGADTVMFFQLRRSFGACEKYHGAVIAHCGRPETRVFRETAQLGAELK
                     KLGDRILESRSRAKAALLFDWENWWAIEFSSGPSVDLKYISQIEKYYQTFFEMNIAVD
                     LVQSTADLSQYDLVVAPVLYMIKPGVAQNIEKYVAGGGTFVTTFFSGIVNENDLVTLG
                     GYPGELRKLLGIWVEEIDALPPEMKNRIVFEKPRAGFEHEYECGLLCDLLHPESAEIL
                     AVYGKDFYQGMPTLTRNIFGKGRAYYIASDPDPRFINDLMQSICKDKGITGPVAPVNG
                     VEVTQRFKDNSEFTFILNHNDHEVTIDLNRGNFRDLISDREIRDKITLAPKDVVILER
                     A"
     gene            5986..6804
                     /locus_tag="VF531_12025"
     CDS             5986..6804
                     /locus_tag="VF531_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7714732.1"
                     /translation="MKPLFPFVTAEEGRLPFYLKSIGKNQNQETIHRPAGHPDFHWLH
                     STQGKGVLIIAGKKYIISEGMGFFFCPGIPHDYFAMEEPWETYWLTFQGYAVPDLLKV
                     LEFAEWGVYHLSDTRILERLMNELYLLVQSNNPLKGYESSALLYRFLLQLKYSTSISG
                     SRSKLLQHERIQPVVTYIEHNYQNSPSLEEMAEIIGVTPQHLCRLFRNAFQMRPFTYI
                     TRVRIKKAKEILIGEDLPLKEVAQRVGYSDTSYFCSIFKEQEGMTPTEFRKIHR"
     gene            6919..>7756
                     /locus_tag="VF531_12030"
     CDS             6919..>7756
                     /locus_tag="VF531_12030"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX7714733.1"
                     /translation="MSISKRAFGKTAEGQEAAIFDLVNSNGMTAAITNFGGIVVSMQV
                     PDKNGKLTDVVLGFDKLEDYEKPGPYFGAIIGRHANRIEDSRFTLNGVEYRLYANENE
                     THLHGGLKGFDKVLWQAGIVKKNGMDSLRLTYLSRDGEEGYPGNLNVRVEYTLSEDNA
                     LEIEYFAKSDRDTVVNLTNHSYFNLSGHGSGDILRHELHINADRFTVNDSRALPTGEI
                     RPVQGTPMDFTRMTPVGLGIDSQYEQIQYGHGYDHNWVLNMSGQSPEKAAEVYDPASG
                     RVL"
BASE COUNT         2041 a         1785 c         1984 g         1946 t
ORIGIN      
        1 cataaagtac ggcaaaccgg tgggggcaac ccctaatggc cgtcatgccg gaacccccat
       61 atcttttggg gccaaccccg atcccggctt tcgcaaggat tccgctccaa cggccatggt
      121 caaggcggtc gccagggtac agtcgggata tggcaatacg gtaccgattc aactggagct
      181 ggatccgggt ctgtcccggg aggaaggggg catcgaaaag gtggccgacc tgattgaaac
      241 ccattttaag ctcggtggca ctcttttcaa tatcaacgtg atcgatgccg aaaagattcg
      301 ggaagcgcac cgcgatccca caaaatatcc cgacctggta gtgagggtta ctggatttac
      361 ggcttatttt gccgcgctat ctccggaatt ccggcaactg gtggtggatc ggatcatcca
      421 ggagtagcac gcccaatttt atttgcgttt tgaacttcat caccggccgg attgatattc
      481 aatccggtct ttaatgttaa tttacaagtt aataatatgg aatggaatat ggaatgatag
      541 atcggcgatg ccgacatccg tataacgatg ataatcggag acagaccatt gactggttaa
      601 ctttaaagtc atataattgc ttcagcaaca acttgaggtg caatgtgaat attgaagtat
      661 ccgtcaaaaa tatgaacttc cttgaggcct tcgcttcgga aacccgggtg aaaatcattg
      721 agttgctgaa tgagaagccg ctgaatgtaa aagagatcgc ggcctatctc gacatttcat
      781 cggctatcgt cacccgccat atacaaaagt tggagcaggc cgggatcatt aaatgtgaaa
      841 actccgcggg gaaaaggggg acccaaaagg tttgttccct caaactggaa caggcgaccc
      901 tgcagtttaa gttcaaagta agtgaagcga atgccaatac gtattgctgt tcgattccgg
      961 tggggcaata ttcgtcctat gacatcaagc ccacttgcgg gctggcgtca accggcggag
     1021 tcatcggggt cgtcgatgat ccacggtatt ttgcggatcc ggatcatatt caggcgggga
     1081 ttatctggtt tggccaaggc tttatcgagt accggatccc caactacttg ttaagcaacc
     1141 agcgggcgaa gttcgttcaa ataacgctgg agatatgttc cgaagctccg ggttataacg
     1201 aagattggcc ttcggagatt accttttatc ttgccgggag agaactgggt ttttggacct
     1261 ccccgggaga ttttggaagc agtaagggta ttttcacacc ggactggtgg aatctgggta
     1321 cccagtacgg actgatgaaa acgattatta tcaatgaaac cggaagttac gtggatggaa
     1381 ttcaaatctc gaccgttacc gtcaacgact tggatattcg ttacggtaag gagatattat
     1441 tccgcatggc tagcctggta aaaagcaaga acagtggcgg tgtgactttg ttcggtaaag
     1501 gtttcggtaa ttacagtcag gatatccagg tgaagatagg gtattaagtt tttgattggt
     1561 ggaggaacta acggatggcg aatcatatac ctgagaatga tactgataaa gtaataaaag
     1621 ttaataaaga gatttcgatc ccgctctata gtcagatcaa aaccattatg ctgcaatcca
     1681 tcgcttcagg cgagtataaa ttgggagaaa aaataccgtc ggaaatcgaa ttggcaaaaa
     1741 agtacggtgt cagccggatc accaccaaac aagcgttgaa tgacctgatg caggagggga
     1801 tcgtcgatcg tgtagccgga aaaggcacgt ttgtgagacg ggcggcaccg ctccggaacg
     1861 atacagcgga ttcaccagtc aatccgcagc ctttgcgggt tcgtggcggt cagccggggt
     1921 ctttcggttt gatcgtcccg tttatgaagg atacctatga aatcgggatt atcagcggag
     1981 tcgaacaaga attacgggac cgggattacc gtttaatctt ccggaaaacc gataactcca
     2041 tcattgaaga gagcaaagcg attgagagtc tgcgtaatga agaagtgcaa ggattgatca
     2101 tctttccggc agagggaatg tactgtagtg aagcgttact caagttaaag ctggagcaat
     2161 tcccgtttgt aatggtggat cgattctatc gaggcatcga atgcaattat gtggtctctg
     2221 ataatttcgg cggcgcctat gccgcggggg agtacctgat ccacttgggc cacaaggagt
     2281 tcgggctcat cggttaccag ccggaacttg ccgttagcat caatgaccgg attcaagggt
     2341 tctgccaggc actggcggat caagggatcc ctttgtcgca gggacggaag gaactatcga
     2401 tgattcggga acaaacttat gatcgtcaca ccggccaggc cgatccggtg gcaattcaga
     2461 ccgttcaagc tttcttggag aggaatcccc ggattaccgc catctttgcc atcaacgatt
     2521 acttggccat ttgcgtctta cgagccgcac tgcagatggg gctcaatgtc ccgcgggatc
     2581 taagtgtgat ggggtttgat aataatattc ttgcggccaa tgtagaggta ccgttgacca
     2641 ccgtcaatca accaaaggaa gagatcggca agagcgcggc gcaactcctg caagggatga
     2701 ttgaaaccga gaatccggtc atggagcatt tggtattatc aacggaatta attatccgcg
     2761 cctccaccgc cccgcccgga gaaagtaaac gggtctaaac ccggcctgcg atctcctgaa
     2821 gtttttgaat actcgcggcg accgtctccg gggtggtttc ttccagcagc atattgatat
     2881 agggtttacg ggattggacc ttttttagca ggtattcaaa atgaaaaagt ccgtctccga
     2941 tgggtgccga gttcttttgg ccatgctcga tagtaaaatc ctttagatgt aacaccacaa
     3001 tccgtttccc gtattgctca aaagcctgat cgagaatgcg gtcctgatcc tcatagttct
     3061 ctacggtcag taggttgacc ggatccagaa tgacttggag attgttggat tggatcgtat
     3121 cacagacttg cttcatcctg tccggactgg aaaccacatg actcgccaca ccctcaatcc
     3181 ccacaaaaac cccgaacttt tcagcctccg tcaccagttc cgtgaggctc tccaaaaata
     3241 accggagtgc cgtttctccg tggttgtccg ggtgaaacga gtagtctcca ttgagagatc
     3301 cggtttccgt tccgaccaca ctgcaaccaa aatccctggc atagcggaga tgttccttga
     3361 atcttgcaat ataatcccgg cggatacccg gatcgggatg gatcaggttg ttgtaacacc
     3421 ccagaaccgc gatctgaatg ttatgattgg caaatgtatc accgatataa cgggccagac
     3481 cggggctgaa actacccggg ccggcatcaa gaccggcgac ggctttgccg agcgccagtt
     3541 ggatactggg tattccccga tctgagatgc gtttggccaa ttcttcgaca ggtaattttc
     3601 caaaatcgtg agcgcgtaca ccgaaacgga acattctatt tcacctcatg aaggtcaatt
     3661 ttgggaccgg cttcgctaaa ccgagcctga gtctaaaaag gattcggtta agcccgttcc
     3721 aagatgacga cgtctttcgg ggcgagcgta atcttgtccc ggatctccct atcggagatg
     3781 agatctctga aattaccgcg attcaggtcg atggttacct catggtcgtt gtggtttaag
     3841 atgaaagtga attcgctgtt atctttaaac ctctgagtta cctcgactcc gttaactgga
     3901 gccaccggac ccgtaatccc cttgtcttta caaatggatt gcattaaatc attgataaaa
     3961 cgagggtcgg gatcggaggc tatataatac gcccggcctt ttccaaatat attccgggtt
     4021 aaggttggca tgccctgata aaaatctttg ccgtagactg ccagaatttc ggcggactcg
     4081 ggatgtaaaa gatcgcaaag caatccgcat tcatattcat gctcgaaacc ggcccgaggc
     4141 ttttcgaaaa cgatccggtt tttcatctcc gggggcaggg catcgatctc ttccacccag
     4201 attcccaaaa gctttcggag ttcaccggga taaccgccca aagtgaccag atcattctca
     4261 ttgacgatac cgctaaagaa ggtggttaca aaggtgccgc cacctgccac atacttttcg
     4321 atgttttggg ccactcccgg tttaatcatg taaagtaccg gtgcgactac cagatcgtat
     4381 tggcttaagt cagcagtgga ttgcactaga tccaccgcaa tattcatctc gaaaaacgtt
     4441 tggtagtact tttcgatctg ggatatgtat ttcagatcga cgctgggtcc actggaaaat
     4501 tcgattgccc accagttttc ccagtcaaag agcaaagccg ccttagcccg ggaccgtgac
     4561 tccaaaattc ggtcacccag ttttttaagc tcggcgccca attgggcagt ttccctaaat
     4621 acgcgggttt cagggcggcc gcaatgggcg atcaccgcac cgtgatactt ttcacaggcg
     4681 ccgaaggacc ggcgaagctg gaagaacatt accgtatccg cgccgtgagc gatggcctga
     4741 tagctccata accgcatgac accgggtctc ttcagactat tgtaagcctg ccagttttgt
     4801 tggctggggg tctgttccat cagcatgaaa ggtgctccgt ctttcaggcc gcgcatcaaa
     4861 tcgtgacgta aggcaatatt tgcgggtaga gcatttaggc tcgggtaatt gtcccaggaa
     4921 acaatatcca tatgtttggc ccagtcaaaa tagtccaatg gtttataagt gcccatcagg
     4981 ttggtagtga ttggaatgtt cggtgtaatg gctttgatgg cttgatattc ccctaaatag
     5041 cactctaaaa tggattccga catgaaccgg ttgtaatcga gcgagatccc ttgaaaacag
     5101 gtgaattccg caggcggtac gcgccaggtg gccatgccca tctcatttaa gtacgaggga
     5161 gggacaatct catcccattc ataaacggta tgaccccaaa aactcatatt ccagcgtttg
     5221 ttgagttctt caatggtgcc gtaacgcttt ttcaaccaat tacgaaattc tgcggcgcag
     5281 tgttcgcaat agcaatagtt accaccgtat tcattggcga tatgccagac caataatgcc
     5341 gggtggtctt tataccttag tgcaaggtgg gaagctaatt tggtagagaa ttcgcggtaa
     5401 acttgactat tggggcaaaa gtttactcga ccgccgtggg tctttttacg cccttggata
     5461 tccacaggca agatctccgg atacttcagg gacatccaag caggctgggc cgcagtggat
     5521 gttgccagac atacatagat gccatttttc gccgccagat ccaacacccg atccagccat
     5581 tcaaaatcat accggtcttc tgcgggctga agcttcgccc aactaaaaac gggaacagta
     5641 atgacattaa tcccggcgag cttgaaaagt cgcatatcct cctgccagac ctcctccggc
     5701 cactggtcag gattataatc tccaccgaaa taaatggaag gaagtttcga attgatcatg
     5761 aggaacctcc ggtaaatttt tttaggatac accgtgtttc atctggatgt ttgtaagcaa
     5821 ttggcaatct tgcctgaaga ataaagtttg aatataatta tatgaaaccg gaagcagtta
     5881 attgtggatg atcttcatta acaccattgt attttaaaac aagcgggaag gcaatccaaa
     5941 aatattcacc cggatatgac gatatgaacc atggagggga tttcgttgaa accgttattt
     6001 ccatttgtca ccgcggaaga gggcagacta ccgttttatt tgaaaagtat cggcaagaac
     6061 caaaaccagg agaccattca tagaccagcg ggacatcccg attttcattg gcttcattcc
     6121 acccaaggta aaggagtcct gatcattgcc ggtaaaaagt atatcatctc ggaagggatg
     6181 ggctttttct tttgccctgg tattcctcac gattatttcg ccatggagga accgtgggag
     6241 acttattggc tcacatttca gggttatgct gttccggacc tccttaaagt cttggagttt
     6301 gcggaatggg gggtgtatca cctcagtgat acccggatct tggaacggct gatgaacgag
     6361 ctctatctgt tggttcagtc caataatcca ctaaagggat atgaaagttc ggcgttgtta
     6421 tatcggtttt tgttacagct aaagtactct accagtatta gtggcagccg ttccaagctt
     6481 ttgcagcatg agcggatcca gcctgtcgtc acctatattg aacataatta tcagaatagc
     6541 ccttcactag aggagatggc ggaaataatc ggtgttactc ctcaacattt atgcaggcta
     6601 tttcggaatg cgtttcagat gcggcctttc acttatatta ccagggtaag gataaagaaa
     6661 gcgaaggaaa tactcattgg agaggaccta cctctcaaag aggtggctca aagagtcggc
     6721 tacagtgata ccagttattt ttgcagtatt ttcaaggaac aagaagggat gacacctaca
     6781 gagttccgaa aaatccatcg ctgagctaag aaccaaggga ttatgtggga aggtgaaggt
     6841 gtgatataat aagcctatgt ctagcatctt cggcaatgga ttacattaca tgacaatgaa
     6901 ctggaggggc atataggtat gagtatttcc aaacgggcat ttgggaagac ggccgaaggc
     6961 caggaggcgg cgatttttga tctcgtcaac tccaacggaa tgaccgcggc gattaccaac
     7021 ttcgggggaa tcgtggtttc aatgcaagtt cccgataaaa atggcaaatt aaccgatgtg
     7081 gtgcttggct ttgataagct ggaggattat gaaaaacccg gcccatattt tggggccatc
     7141 atcggacggc acgccaaccg gattgaggat tcccggttca cgctgaatgg agtagagtac
     7201 cggctgtatg ccaatgaaaa cgagactcat ttgcacgggg gtttgaaggg cttcgacaag
     7261 gttctgtggc aagcggggat cgtaaagaaa aacggcatgg attccttacg cctaacttac
     7321 ctaagccggg atggagaaga gggttatccg ggaaatctca atgtcagggt agagtatacc
     7381 ttgtccgagg acaacgcctt agagatcgag tattttgcca aatccgaccg ggacactgtg
     7441 gtcaacctga ccaatcattc ctatttcaat ctgtcggggc acggatccgg cgatattctg
     7501 cgccatgagt tacatatcaa cgccgatcgg tttactgtca acgactcccg tgctctgccg
     7561 accggagaga ttcggccggt acagggtaca ccaatggatt ttacccggat gacaccggtc
     7621 ggtctgggaa ttgacagcca atacgaacag attcaatatg gccatgggta tgatcacaac
     7681 tgggttctga atatgagcgg tcaaagcccc gagaaggcgg cggaggttta tgatcccgca
     7741 agcggccggg tgcttg
//
LOCUS       DASVMB010000427         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_199247,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000427 DASVMB010000000
VERSION     DASVMB010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199247"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VF531_12035"
     CDS             complement(<1..1187)
                     /locus_tag="VF531_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7714734.1"
                     /translation="MFHFNRTTGQSGGLNRLALIITIVMGIILGFVLVLTFGTNKGLL
                     SLPPSKQATIIYDINKNEYTRLFDENRMEVPLQKMPVYLKKGIVAVEDNRFYEHSGID
                     LRAIFRALWVDIRGGGYIEGASTITQQLARNVLLTQKKAISRKVQEIFLAMTIERNYT
                     KEEILERYLNQICFGHGTYGVESASRLFFGKDVTELQLHQIALLIGLPKNPTGFSPYL
                     HPQSSRGRRTVVLELMTKYGVITQAQAEEANRMPLDVIPLSTSKRRAPYFVDYVIQRL
                     KGIINEEALYTGGYKIYTTIDPMAQQAAEDAATLISGGKPDSVGVFQPQIAIVALDPR
                     TGYIKAMIGGRDFGNTQLNRAALAARQVGSAIKPFVYTTAIDSRRFTPSSVLVDEEIE
                     YPTP"
     gene            1390..2088
                     /gene="yunB"
                     /locus_tag="VF531_12040"
     CDS             1390..2088
                     /gene="yunB"
                     /locus_tag="VF531_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HEX7714735.1"
                     /translation="MNFHLKKRIITFRVPPYQKNFFRSLKFLGIFCLVMVGVFYIVDF
                     RIRPTLLQLAMTQAKVIATRAINEAIRADITPDIQYQNLIKLQLNTEGQVTLIQPNTG
                     EINRISSEATLAVQKRLENLPNEVIRIPFGQIAGSKIMAGFGPKIPVKVIPIGFVTSN
                     INDRFVSTGINQVRHQVFLTINATIKLVVPLVNQEVRVSSDVPLVETVIVGEVPQVYV
                     ENGDGVILPGTPAK"
     gene            complement(2162..3370)
                     /locus_tag="VF531_12045"
     CDS             complement(2162..3370)
                     /locus_tag="VF531_12045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEX7714736.1"
                     /translation="MESLREIYRIGMGPSSSHTMGPHRAALIFGARYPGAVGFRVTLY
                     GSLAATGKGHLTDKAISDALIGRVISFCWETERLLPLHPNGMEFEAFGPGGELLGFWR
                     VYSVGGGALLDEGQSLLQDDRYPQRTMAQFLQDMEKTGRTFWQLALAAEDTSFPDYLN
                     EVWTVMTQAIERGSTTNGVLPGRLRLERKSARVLAGQIPGEPLYRSNRLFAYALAVAE
                     ENAAGGQIVTAPTCGSSGVLPAVLRFLEETHHLPRRQIIEALATAGIIGNLVKSNGSI
                     SGAAVGCQGEIGTACAMAAAAAAQLLGGTPRQMEYAAEMGIEHHLGLTCDPVAGLVQI
                     PCIERNAFAATRALDCAEYALLSGDKHRISFDEVVQTMMQTGQDLKCSYRETATGGLA
                     ALHDPSQFQK"
     regulatory      3627..3767
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3841..>4406
                     /locus_tag="VF531_12050"
     CDS             3841..>4406
                     /locus_tag="VF531_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7714737.1"
                     /translation="MSKTLVTVEQVTLSYVSDTPPELEGVDLELHRGEILSLVGPSGC
                     GKSTLLRLIAGVLKPTAGEIRIGNRQAFPGWPGLGFVPQDSLLFPWRTVLANVRLPLE
                     LKGKMPEEAMNRLARAALNLVNLNQADNKYPHQLSGGMRQRAALARALVSTICHDADF
                     AGATFDNTGEAKTINGNIPGNIFGNSIMP"
BASE COUNT         1098 a         1138 c         1070 g         1100 t
ORIGIN      
        1 ggggtcgggt attcgatctc ttcatccacc agcacgctac tgggggtgaa acgtcgactg
       61 tcaatagctg tggtatatac aaaaggttta atagccgaac cgacttggcg cgcagcgagt
      121 gccgcccggt tcaactgggt attgccaaaa tctcttcctc cgatcatggc tttgatatag
      181 ccggttcgtg gatccagagc cacaatcgca atctgaggct gaaatacccc gacagagtcg
      241 ggcttaccac cgcttatcag agtggctgcg tcctctgcag cctgctgcgc cataggatcg
      301 atagtggtgt agatcttgta tccccctgta taaagtgcct cttcattgat aatgcccttt
      361 aaacgttgaa tgacataatc cacaaagtag ggggcccttc gttttgatgt cgataacgga
      421 atgacatcga gcggcatccg gttggcctct tccgcctggg cttgggtgat caccccgtat
      481 ttcgtcatta actctaagac aaccgtacgt cgtccccggg aagactgggg atgcagatag
      541 ggagagaaac cggtgggatt cttgggcaac ccgatgagca acgcgatttg atgcaattgc
      601 agctcggtga catctttccc aaaaaagagc cgcgaggcgg attccacacc atatgtaccg
      661 tgaccgaaac agatctgatt cagataacgt tccaggatct cttccttggt ataattccgc
      721 tcgatcgtca tcgccaggaa gatctcctga actttacggg agatcgcctt tttttgagtc
      781 agtaacacat tccgggccaa ttgttgtgta atggtgctgg ccccttcgat gtaaccgcca
      841 ccccgaatat ctacccataa ggcacgaaaa atggccctca gatctattcc ggaatgctca
      901 tagaaacggt tatcttcaac tgcaacgatt ccttttttca gatacaccgg catcttttgt
      961 aaaggaacct ccatccggtt ttcatcgaag agtctggtat attcattttt gttaatgtca
     1021 taaatgatcg ttgcctgctt ggagggaggc agacttaata agcccttatt ggtcccaaag
     1081 gtcaacacca gaacgaaacc caatataata cccattacaa ttgtgattat cagcgccaga
     1141 cggtttaacc caccggactg cccggtggtt cggttaaaat gaaacatcaa tgatccccct
     1201 gtcggactgg cattatgaaa ctcgttttga agtatatctt ccattatacc ataaggtgga
     1261 acgatatagt taaatcaagc aatacaaagc aagcttggta tcatttgcct ggcagaatga
     1321 tgaatttttc catagggtat gaaataaaca ccatgattgc cacatatgga taattgaggc
     1381 gatggcggaa tgaattttca tctaaagaaa cggattataa cctttcgggt tcccccttat
     1441 caaaaaaact tctttcgttc cttgaaattc cttgggatat tctgcttggt catggttggt
     1501 gtcttttata tcgtggattt ccggattcgt cccacacttc ttcaactggc catgactcag
     1561 gccaaggtca ttgccacgcg ggccatcaac gaggcgattc gggccgatat aactccggac
     1621 attcaatatc aaaacctgat caaacttcag ctgaataccg aagggcaggt gacgctcatc
     1681 caacccaata ccggggagat caaccggatc tcctcggaag ccacgctcgc agtgcaaaaa
     1741 aggcttgaaa atctcccgaa cgaggtgatc cggattcctt tcggccaaat cgccggatcg
     1801 aaaattatgg ccggcttcgg acccaaaata ccggtgaaag ttattcccat cggttttgtt
     1861 accagtaaca tcaatgaccg gtttgtttct accggtatca atcaagtccg ccatcaggtt
     1921 tttcttacaa ttaatgccac cataaagctg gtcgtgcccc tggtgaatca ggaagttcgt
     1981 gtatccagcg atgtcccgct ggtggagacg gtgattgtcg gagaagtgcc tcaagtttac
     2041 gtagaaaacg gagacggtgt catcctgccc gggaccccgg caaaataact cccgcccggg
     2101 ctaaaaaccg gttggaaatg aaagactatc cataaatgcg gctgacgctc aaatcaataa
     2161 tttatttttg gaactgtgaa ggatcgtgta aagccgccaa tccaccggta gcagtctccc
     2221 gatacgaaca tttcaaatcc tgcccggtct gcatcatggt ttgaaccact tcgtcaaagg
     2281 atatcctgtg tttgtccccg gagagtagcg catattccgc acagtccaat gcgcgagtag
     2341 cagcaaaggc attccgttcg atgcagggaa tctgcaccaa tccggcgaca ggatcacaag
     2401 tcagaccgag gtgatgttcg attcccattt ccgcagcata ctccatctga cggggagttc
     2461 cgcccagaag ttgtgccgcc gcggcagcgg ccatggcaca agcggtgccg atctcgccct
     2521 ggcatcccac ggcggcaccg gagatggatc cgttcgactt taccaagtta ccgatgatcc
     2581 ccgctgttgc caaggcttca atgatttgac gccttggcaa gtgatgggtt tcttccaaaa
     2641 agcgaagcac cgccgggagc actccactgg aaccgcaggt aggggcggtg acaatctggc
     2701 caccagccgc attctcctcg gcaaccgcca gagcataagc aaaaagacgg tttgatcgat
     2761 ataacggttc accggggatc tgaccggcca aaacccgggc gctttttctt tccagccgca
     2821 accgcccagg taaaacgcca ttggtagtgc tgcctcgttc aattgcctgg gtcattacgg
     2881 tccagacttc attcaaataa tccggaaaag aagtatcttc cgcagccagt gccagttgcc
     2941 aaaaggtccg accggttttc tccatatcct gaaggaattg agccatcgta cgttgcgggt
     3001 atcgatcatc ctgtaacaag ctttgtccct catccagtaa agcgccgccg ccaacactgt
     3061 aaacccgcca gaaaccaagt aactcaccgc cggggccgaa tgcctcaaac tccatcccgt
     3121 tagggtgcag gggtaacaag cgctctgttt cccaacaaaa gcttatgacc cgcccaatca
     3181 acgcatcact gattgccttg tcggtcaaat gtcctttacc cgttgccgct aaacttccgt
     3241 ataatgtgac ccgaaaaccg actgccccgg ggtatcgggc tccgaagatt aaggcagccc
     3301 ggtgaggtcc catggtatga ctggatgaag gtcccatacc gattcgatat atctcccgga
     3361 gtgattccat tatagattcc tttccgcggg cttttttgct gcagcaatcg tggagtaagt
     3421 cgcgccggag gtattttgaa ttctcttcat cattatatac tttcgcaact tgggtataca
     3481 atggtaacac tgacccgata ttttatggcc acagggtccg gcccggattt ttaacccaac
     3541 tgtcaccatt ttgttaatca aaacatggct tcctctattt acaaatggtt ccgatatggt
     3601 aaaataaagt tgttccgaaa atgaatatta cccttcaggg cagggtgcgg cgtgctggta
     3661 gccaattccc gaccggtggt aaagtccacg agcggttctt tttaatgaat cgcagatctg
     3721 gttagattcc agaaccgacg gtagagtccg gatgagagaa ggcagattgt tgacaaaagc
     3781 cctgaatatt tctattcagg gcttttttat tggacggtga gtgctttggg tgacagtgat
     3841 ttgtcaaaaa cgttggttac ggttgaacag gtgactctat cctatgtttc cgatactcca
     3901 ccggaacttg agggggtcga tttggaatta caccgcggtg agatcctcag tttagtcggg
     3961 ccgagcggtt gcggcaaatc aaccttattg cggctgattg ccggggtctt aaagcctaca
     4021 gccggggaaa ttcgtatcgg caaccgtcag gcctttccgg gttggcccgg gttggggttt
     4081 gtgccccagg actcactgct attcccttgg cggacagttc tcgccaatgt ccgcctaccg
     4141 ctggagctaa aagggaagat gccggaggag gcgatgaata gattggcccg ggccgcgctt
     4201 aatctggtca atctgaacca agcagataat aaatatcccc accagctttc aggggggatg
     4261 cgtcaacgcg ccgctctggc ccgggctctg gtcagcacca tttgtcatga cgctgatttc
     4321 gccggcgcca ccttcgataa taccggtgaa gctaagacca tcaatggtaa tatccccggg
     4381 aatattttcg gcaattccat catgcc
//
LOCUS       DASVMB010000428         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_422032,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000428 DASVMB010000000
VERSION     DASVMB010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422032"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF531_12055"
     CDS             <1..447
                     /locus_tag="VF531_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714738.1"
                     /translation="FKNLGMYNSHLPLILPAFFGNAFGIFLLRQYFLTIPEELNDAAK
                     VDGCGTFRLFYQIYLPLVKPVLITLAIITLQGTWGDVLNPLIYITDQSLFTVVLGLRL
                     IARSQFLPQPQLEIAGNLLLILPIMLIYLFAQRYFTQSIQTTGIKG"
     gene            530..1816
                     /locus_tag="VF531_12060"
     CDS             530..1816
                     /locus_tag="VF531_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714739.1"
                     /translation="MKKIWNVLLIIALCLSVVSFGYAKAPVKIRIAYYGDQKPAENMA
                     GIINEFQKANPNIKVDINWTDWDSHYERLRTELAAGSGPTVYLLDSVYIQGYAGRGLL
                     EDLSTKVKVFSKDKYSGLDAVKDPKGKTWAVPQGIQVQVLYYNKDMFDKAGVQYPTAD
                     WTTTDMLAAAQKLTVDTNGDGKIDQWGICLPNHIRFGWYGLIRMFGGDIMDKTRTKST
                     INKDPQVRAALQYMHDTWYKYKVSPTLQDAEGVLTPRYYTYFARKIVAMWMDNFSRRI
                     DSDQAGINYDIQVVPKGPGPKGVHYSAIVANSWVINKQASKAEKDAGWEFIKYYMTDK
                     GQSLYAEGGEALPSSKAACAAMLKKLDKPEHNMIYLDTMKYTDTMGENAVWEEWYQVV
                     QDGLQEYLTNKVSLDDCLNRLNVNLQKVLDDWYKKK"
     gene            complement(1867..2919)
                     /locus_tag="VF531_12065"
     CDS             complement(1867..2919)
                     /locus_tag="VF531_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7714740.1"
                     /translation="MDKIKASALKSVFSLLLSITAILVLVLSKSYARSDVVTSMNKPV
                     TGVVKANGINIAYESYGSPDCETILLISGTGAQLIDWPVELIEELVKQDYRVVCFDNR
                     DVGLSTKFDEAGYPDLTAIGKALEKGEPAPIPYTLNDMADDAVGLLDALSIQKAHIVG
                     VSMGGAIGQLVAINHPKRTLSLTSIMADSGNPKLPVFAKPEVFTPLPQLSPDISMDDF
                     IDYRIKTTQVLCSPGYPLDEKSLRKQVQSDVARSYNLAGFMRQQIVSLVGHYTNNING
                     YRQKDLKMIKVPTLVLQGENDPLVPIESARDIAANIPGAELIIISGLAHDLPEQLAPI
                     IAGAIVTAARQTGSPV"
     gene            complement(2956..3951)
                     /locus_tag="VF531_12070"
     CDS             complement(2956..3951)
                     /locus_tag="VF531_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714741.1"
                     /translation="MNLIDRYVYAVTERLPANTREDVAKELRSNIEDMLPKNATAEDV
                     RAVLVKLGNPIKLAAEYNHEKKYLIGPGLYEKYISTLKLVMGVTSAAVTLIYLLGIIF
                     KPSADIGAFNLSAVTFVQIFAAIFQILTQVFLWVTLVFIVLEKTGISAGRLPFPKQEW
                     SPDALPSIPIPEKKRISRYEAIFSLSFTIVFILLIYSQPQFIGWYEKGSHGFTLVEPF
                     FNAERLQTYIYIVIILAIIQLFISIYKLTVIYWNIQLAVVVAVYNAVLCMLAVTMIND
                     STILNQGFLLHFSSAVASTVPEASRMWQIGGWIFAVIFLGISIWDSIANFHKCKV"
     gene            complement(3953..4297)
                     /locus_tag="VF531_12075"
     CDS             complement(3953..4297)
                     /locus_tag="VF531_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7714742.1"
                     /translation="MSTVDELLNSFIIELRRGTLVLSVLSQLSEPEYGYSLIQKLDSK
                     NSSIEAGTLYPLLRRLEKQELLISKWDTNESRPRKYYVLSDKGREIYLCLKNEWENLS
                     KQLEGLLDKEEK"
     gene            4546..5394
                     /locus_tag="VF531_12080"
     CDS             4546..5394
                     /locus_tag="VF531_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEX7714743.1"
                     /translation="MTQVSNCSVRVGNVEEFLSQIDQAYNTLCDISQGSQCELWFRGQ
                     VNQNFTLLPSIGRPPINNATLEDAYLSKFQSLAIPYIQNLPAFPLPNGVPSYWSWLVL
                     MQHYGVPTRLLDWSRYALAALYFATNPLDTGIQPGVDGVVWVLNPAKLNQAFSFNPNL
                     QPGYIPNMEEAGFTQIFGPQSQNLDNLKPAAAIAPVNNPNIMAQRGTFIVYPLKKNLI
                     PLENFEDASDYLIKICIASESFALIHTQLQHYGITELALFPNITNLAREINQQVQNEG
                     NRPTDP"
     gene            complement(5563..6195)
                     /locus_tag="VF531_12085"
     CDS             complement(5563..6195)
                     /locus_tag="VF531_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX7714744.1"
                     /translation="MKSKEQMKRLIIRILAISFGLFINGIGLAFLYSVELGSSPMGTF
                     ADGLHNLIHISQGSANILANAAFLLVLLILARKYISVGTVLCTFTLGLYVNLASAVIG
                     PLNLVALPFLNKIMVSAVGTLLMGVGLGIYVAVDFGLGPLEAIVAIIYHHSKLKYKTA
                     KIIFDALLGVFGIVLGGKIGIGTVISILCTGMVMDPVIAKTKPFLAKIKA"
     gene            6573..>6765
                     /locus_tag="VF531_12090"
     CDS             6573..>6765
                     /locus_tag="VF531_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714745.1"
                     /translation="MGSTIKDVAKKAGVSPTTVSLVFNNRESRVSETTRERVMAAAKE
                     LKYYPNHFAASLVTKRSHII"
BASE COUNT         1933 a         1528 c         1523 g         1781 t
ORIGIN      
        1 tttaagaacc tcgggatgta taattcacat ctgcccctga ttttaccggc ctttttcggc
       61 aacgccttcg gcattttttt attgcgccaa tactttctga ccatccccga ggaattgaac
      121 gatgccgcca aggttgacgg ctgtggtact ttccggctgt tttaccagat ctatttacct
      181 ttggtcaaac cggtcctgat taccttggcc atcataaccc tgcagggaac ctggggcgat
      241 gttttaaatc ccctgatcta cattacggat cagtcgttgt tcaccgtggt tttaggcctg
      301 cggctcattg ccagaagcca gtttctgccc cagcctcaac tggagatcgc cggcaatctg
      361 ttactcatac tcccgatcat gctcatctat ctatttgctc agcgttattt cacccaaagc
      421 attcaaacta ccgggattaa gggttagtcc tcatcttatg gaagggaggt gttgacatcc
      481 ggcttttcca acatagataa atcagattga aaaagagagg agaaacgtaa tgaaaaagat
      541 atggaacgtc ttgctcatca tcgctctttg tttatcggtt gtttcatttg ggtatgccaa
      601 ggctccggtg aagatccgga ttgcgtatta cggggatcag aagccggccg agaacatggc
      661 gggcattatc aatgagttcc aaaaggctaa tcctaatatc aaggtggata tcaactggac
      721 cgattgggat agccattatg aacggttaag gaccgaattg gcggccggca gcggtccgac
      781 ggtatacttg ctcgatagcg tatatattca aggatatgcc ggcagaggcc tgcttgagga
      841 tctatccacc aaagtcaagg ttttcagtaa ggacaaatat tccgggttgg acgcggtgaa
      901 ggatccaaag ggcaaaacct gggctgtacc gcaagggatc caggttcagg tcctgtacta
      961 taacaaggat atgttcgaca aagccggggt gcaatatccg accgccgatt ggaccactac
     1021 cgatatgttg gctgcggcgc aaaagttaac ggttgacacc aacggcgacg gcaagatcga
     1081 tcagtgggga atctgcctgc cgaatcacat ccgttttgga tggtatggtt tgatccggat
     1141 gttcggcggg gatatcatgg acaaaacccg taccaaaagc acgattaata aagatcctca
     1201 ggtaagggcg gccttgcagt atatgcatga tacctggtat aaatataagg tctctcccac
     1261 cttgcaggat gccgaaggag tattgactcc gcgctattat acgtattttg cccggaagat
     1321 cgtggcgatg tggatggata acttttcgcg gcggattgac agcgatcaag ccggtatcaa
     1381 ttatgatatt caagtggttc ccaaagggcc cggtcccaag ggcgttcact acagcgccat
     1441 tgtcgccaac agttgggtaa tcaataaaca agcctcgaaa gcggaaaaag atgcgggctg
     1501 ggagtttatc aagtactata tgaccgataa agggcaatcc ttatacgccg aaggcggcga
     1561 agccttgccg tcgagcaagg cggcctgtgc cgcaatgctc aaaaagcttg ataaaccgga
     1621 acataacatg atctacctgg acaccatgaa atataccgat accatggggg agaacgccgt
     1681 atgggaggaa tggtatcagg ttgttcaaga cggcttgcag gaatatttaa ccaacaaggt
     1741 atcgttagac gattgcctga accgactcaa tgtcaatcta caaaaggtcc tggacgactg
     1801 gtataaaaag aaataacccc cccgtttttt ggaaaagtca catggcagta aaaacaggaa
     1861 gccctttcaa acagggcttc ctgtttgccg tgctgctgtt acgattgcac ccgcaatgat
     1921 gggggcaagc tgttccggaa ggtcatgtgc caatcctgaa atgattatca actctgctcc
     1981 gggaatattg gcagcaatat cacgtgcgga ttctattgga accaatggat cattttcacc
     2041 ctgtagtact aaggttggaa ccttaatcat tttaaggtcc ttctgacggt atccgttaat
     2101 attgtttgta tagtggccta caagagaaac tatttgttgg cgcataaaac cggctaaatt
     2161 ataagaacgg gccacatcac tctgcacctg cttgcggagg gacttctcat ccagtgggta
     2221 gccgggactg cagagcactt gtgttgtttt aatccggtag tcgatgaaat cgtccataga
     2281 gatatcggga gagagctggg gaagtggtgt aaaaacctca ggcttggcaa aaaccgggag
     2341 ctttgggttg cctgaatccg ccatgattga tgtcagcgag agtgttcgct taggatggtt
     2401 gattgcaaca agctgaccga tggctcctcc cattgaaact cccacaatat gtgccttctg
     2461 gatgctgagc gcatccagaa ggcctactgc atcatccgcc atatcattga gcgtataggg
     2521 gatcggagcg ggttcgcctt tttccagtgc tttgccgatt gctgtcaaat caggatatcc
     2581 tgcttcatca aattttgttg aaagtccaac gtcgcggtta tcgaagcaga caaccctgta
     2641 gtcttgtttt accaattctt cgataagctc aaccggccaa tcaatcagct gcgccccggt
     2701 accggagatg agcagtatag tttcacaatc cggcgagcca tagctttcat acgcaatatt
     2761 gataccgttg gctttgacaa cacctgttac aggcttgttc atagaggtca caacatctga
     2821 acgagcataa ctttttgata aaaccaaaac aaggatggcg gtaatactca gtaataaaga
     2881 gaacacagat tttaacgcgc ttgcttttat tttatccaca acacatcacc acaccccttt
     2941 actgaatttc aacttttaca ctttgcattt atgaaagttg gcgatactat cccaaatact
     3001 gattcctaaa aatattactg caaaaatcca tccgccgatt tgccacatac gtgaagcttc
     3061 cgggacagtt gaggcgaccg cactggaaaa gtgtaataaa aacccttggt ttagtatggt
     3121 agaatcattg atcattgtta ccgcaagcat acaaagaact gcgttataga cggcaacaac
     3181 aacggccaac tggatattcc aataaattac agtcaatttg tatatggaga taaagagctg
     3241 aattatggcg agtataataa ctatatatat gtatgtttgc aggcgttcag cattaaaaaa
     3301 tggttcaact agcgtaaagc catggctgcc tttttcatac catccgatga attgtggttg
     3361 agaatatatc aataggatga aaacaattgt aaaagatagg gagaatatgg cttcgtaacg
     3421 ggaaatcctt ttcttctcag ggattggtat tgacggaaga gcatccggcg accattcttg
     3481 tttcgggaaa ggcagccttc ctgcagaaat tccagttttc tccaagacga taaagaccaa
     3541 agtgacccat aaaaaaacct gcgttagtat ttgaaagatg gcagcgaaaa tttgcacaaa
     3601 cgtaaccgct gacaaattga atgctccaat atctgcagag ggtttgaata taataccaag
     3661 cagatagatt aatgttacag ccgcagaagt tactcccatt accaatttta atgttgaaat
     3721 atacttttca taaaggcccg gaccaatcag gtactttttc tcatgattat attccgctgc
     3781 aagctttatc gggttaccaa gctttacaag gacagcccgg acatcttctg ctgttgcatt
     3841 tttaggcaac atatcttcta tattggaacg cagttccttg gctacgtcct cacgggtatt
     3901 cgccggtaac cgctcggtaa cggcatatac ataacgatca attaaattca tttcattttt
     3961 cctccttatc taataatccc tccaactgtt ttgacaggtt ctcccattcg ttctttaagc
     4021 acagatagat ttctcttcct ttgtcactta aaacataata ctttcgagga cggctttcat
     4081 tggtatccca tttacttatc aataattctt gtttttccaa cctgcgtagt aatggataga
     4141 gtgttccggc ttcaatggac gaattcttgc tatctaattt ctggataagg gagtatccat
     4201 actcgggttc ggaaagctgg ctcaaaaccg agaggactaa agtcccccgc cttagttcaa
     4261 taataaaaga atttaacaac tcatccacag tgctcatgta accacctcaa caaaattata
     4321 ctgtgtgaca tacactattg tcaatatatc attataatag aatcattaat attaacaata
     4381 aacttgtcat ttcatgcata aatagataac ataaggaggg tacaaccccg ataaacccgg
     4441 cgattgcagt ggcaaaaaac tgccttaaca atatgccgca tccgaattcc gatccatatg
     4501 atgaaatatg gtcgtgtata gtcatatgga aaggagcgaa tccttgtgac ccaggtttca
     4561 aattgtagtg tgcgagtggg caatgttgaa gaattcttga gccaaataga ccaggcatat
     4621 aatacacttt gcgacatatc ccaaggcagt caatgtgagt tgtggttcag gggccaggtc
     4681 aatcaaaact tcactttact gccgagtatc ggtagaccgc cgattaataa tgccacactg
     4741 gaggatgcct atttatcaaa atttcaatcc ctcgccatcc cttatattca aaacctgccc
     4801 gcttttcctt tacccaatgg tgtaccttcc tattggagct ggctggtgtt gatgcaacac
     4861 tatggtgtac cgacgcgttt attggactgg tcccgttacg ccttagccgc gctttatttt
     4921 gccaccaatc ctctggatac aggaatccag ccgggagtgg acggtgtcgt ttgggtgttg
     4981 aatccggcca aactaaatca ggccttcagt ttcaacccca atttgcagcc ggggtacatc
     5041 cccaatatgg aggaggccgg gtttacacag atttttggtc cccaaagcca gaacctggat
     5101 aatctcaaac cggccgcggc catcgccccg gtcaataacc cgaatattat ggcccagcgg
     5161 ggcaccttca ttgtctatcc gttgaagaaa aacttgatac cgctggagaa ctttgaggat
     5221 gcctccgact atctgatcaa gatttgtatc gcgtcggaaa gctttgccct gattcatacg
     5281 caactacagc attacggaat taccgaactc gccttgttcc caaatataac caatctggcc
     5341 cgggagatta accagcaggt gcagaatgaa gggaaccggc cgacggatcc gtaagatagc
     5401 caggtgaatt gaaaaagaaa tttcaagtgt ccctggagtc cggaagcatg cgcgtgaaac
     5461 tgtgatctga ttcacagttt ttttgtataa gaaaacccca cgcctagcgt ggggttcaaa
     5521 aaaacttacg gcgatgagga taatggaaag ggaaccggat gctcaagctt ttatttttgc
     5581 caaaaagggt ttggttttgg caattacggg atccatgacc atgccggtgc atagaatgga
     5641 aatcacagtg ccaataccga ttttgccgcc cagcacgatt ccgaagacac ctaaaagcgc
     5701 atcaaaaatg atttttgccg ttttatattt tagtttactg tggtgataaa tgatagccac
     5761 gatagcctcg agaggcccca gcccgaaatc caccgcaacg taaatgccca gcccgactcc
     5821 catcagcagg gtgcctactg cggataccat aattttgttc agaaatggca gagctaccag
     5881 gttcagcggg cctatcacag cggaggcaag gttgacatac aggcccagtg taaacgtgca
     5941 aaggaccgta cccacgctga tgtattttct ggccaggatc aaaagcacca gaagaaaggc
     6001 tgcgttggca aggatattcg cacttccctg cgagatatgg atcaaattgt gcaggccgtc
     6061 ggcaaatgtc cccatcgggg aagagccaag ctccaccgag tacaaaaagg ccagcccgat
     6121 accattgatg aaaaggccga agctaatagc tagaattcgg ataattagac gtttcatttg
     6181 ttccttagat ttcaaaatgg gtctcctcac ggtgatgata gttacattta cataaaaaac
     6241 taaaatcagt ttaccgtttt atcaaacgat tgtatggaaa acggcagctg ccttttctta
     6301 ggcaaaataa tactatacaa tcatctcaat gataacaata agcattgtac caggcaaatt
     6361 tgtagtagtc aacaataaac aaaaataatt tttataccag gttacttttc gtacatgtat
     6421 tgactaattc gcaatccatg ctatactata ttttgtaata gataaaacgt taaactaatt
     6481 catggcaatt tctcattccg gaatgcttga cgccgtcgtc cgtagatcat gttccggtcg
     6541 ggatgcgaat cgatataaga aggttgaggg tcatgggttc gacaatcaaa gacgttgcaa
     6601 agaaggcagg cgtttccccg accacggttt ccctggtttt caacaaccgg gaatcaagag
     6661 tttccgaaac cacgagggaa agagtgatgg ccgccgccaa agagctaaaa tattatccga
     6721 atcatttcgc ggcaagtctc gttaccaaga gatcccatat catcg
//
LOCUS       DASVMB010000429         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_276246,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000429 DASVMB010000000
VERSION     DASVMB010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276246"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF531_12095"
     CDS             <1..819
                     /locus_tag="VF531_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX7714746.1"
                     /translation="HFFISSTHENILFFTNQGRVYRLKGYEIPEAGRQARGTAIVNLI
                     PVQPDERVNAVIPIQEFTPDSFLLMGTKSGVIKKTPLDEFQTIRKGGLIAISLDEGDE
                     LIEVKLTDGNAQVIMVTRMGQAIRFPETDVRTVGRGARGVKGITLDEDDQVVGMDIAR
                     EGADLLAITEAGIGKRTPLDDYRIQSRGGKGIKTMKLTKRHGLIAGIKVVADTDELMV
                     ITAEGIIIRTSVKDISQTGRDTQGVKIMKLDKNDRVVTLARVAAGKDDEEVGEE"
     gene            1063..1371
                     /locus_tag="VF531_12100"
     CDS             1063..1371
                     /locus_tag="VF531_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7714747.1"
                     /translation="MKKTLPIEIPKEVLAEYCRKYHVKKLSYFGSVLRDDFNPQSDID
                     ILVEFEPGHVPGLFGLAGMEAELTSLVKRKVDIRTVEDLSPYFRQDVINSAEVGFFEG
                     "
     gene            1361..1708
                     /locus_tag="VF531_12105"
     CDS             1361..1708
                     /locus_tag="VF531_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX7714748.1"
                     /translation="MKDDLTKLHHMLDSAKEAVTFASNHTRDDLNQDRMLTLSLIKSV
                     EIIGEAAARTSAELRTQYPNIPWQNIIGMRNRLIHAYFDINFEMLWNTIQKDLPPLII
                     EVQKVINQESLGK"
     gene            2113..>2813
                     /locus_tag="VF531_12110"
     CDS             2113..>2813
                     /locus_tag="VF531_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7714749.1"
                     /translation="MDREILNLEEAAGLFGVSVKTFIKLLKEEKVPGRKIGREWRFSR
                     GALIDWLSRGDSQSYSSSEGETREFFNEVAPDWEELSRGYYDASIKNKLIEWELLRPD
                     RILLDLGTGDGYIARFAAPSVGKVLALDISGAMLRELERKAQVEGLQNIETVQGDGCD
                     LPFADNSVDLVTANMYLHHIEEPEAAISEIGRVLRKGGAAFLADFVEHGDREMMTRMH
                     DLWPGFAPETVRKWF"
BASE COUNT          731 a          612 c          793 g          677 t
ORIGIN      
        1 catttcttca tctcctccac ccatgagaac atcctcttct tcaccaatca agggcgggtt
       61 taccgcctga agggttacga gatcccggag gctggccgcc aggcccgcgg aacggccatt
      121 gtcaacctga ttccggtgca gccggatgaa cgggtcaacg cggtgattcc gattcaggaa
      181 ttcactcccg atagtttctt gttgatgggc actaagagcg gagtaattaa gaagacgccg
      241 ctcgatgagt tccagacgat ccgcaagggc ggcctgatcg ccatctccct ggatgagggg
      301 gacgagttga tcgaggtgaa actgaccgac ggaaatgccc aggtgatcat ggttactcgg
      361 atgggtcagg cgatccgctt cccggagacc gatgtacgta cggtaggtcg aggggcccgg
      421 ggcgtgaaag gcatcacctt ggatgaagac gatcaagtgg tgggaatgga tatcgcccgt
      481 gaaggcgcgg atctgttagc catcaccgag gccggaatcg gtaaacggac tcctttggat
      541 gattacagaa tacagtcccg gggcggtaaa gggatcaaga ccatgaaact caccaagaga
      601 cacggcctga tcgccgggat caaagtggtt gcggatacgg atgaactcat ggtgatcaca
      661 gccgagggga tcatcatccg gactagcgtc aaggatatct cccagaccgg ccgcgacacg
      721 cagggcgtaa agatcatgaa actggacaag aacgaccggg tggtcaccct ggcccgggtg
      781 gccgccggga aggacgacga agaggtaggg gaagagtagg ggcctatgac cctagcctaa
      841 aagggaattc gctgatcctt cgctctttgg tcagtatgta ttgctataaa agattcgaat
      901 ttcaatgaga gtaaaaaagc cgcttgaaca cttggtcagg cggttttttc ttttaggtgc
      961 caaattcttt gatctcaagt gaaagagggt actgatattt tttttcaaag ataatgtata
     1021 atataatcaa gatgatgagc ggagaagtat ggagggtttt caatgaagaa aacactacct
     1081 attgaaattc cgaaggaagt gttggcggag tattgccgta aataccatgt caaaaagtta
     1141 tcctactttg gttcggtttt gcgggatgat ttcaaccccc aaagcgatat cgatatccta
     1201 gtggagtttg agccgggtca tgtaccgggt ttgtttggtt tagccggtat ggaagcggaa
     1261 cttacaagtt tagtcaagag aaaagtcgat attcggacgg ttgaggattt aagtccatat
     1321 ttccggcagg atgtaattaa ctccgcggag gtcggtttct ttgaaggatg atcttactaa
     1381 acttcatcat atgttagatt ccgctaaaga agctgttaca tttgcctcta accatactag
     1441 agatgatctg aatcaagacc ggatgcttac tttatccttg atcaagtcgg tcgagatcat
     1501 cggggaagca gcagcgagga caagtgcgga actccgaaca cagtatccca atattccttg
     1561 gcaaaacatt attgggatga gaaatcgatt aatccatgct tattttgata ttaactttga
     1621 aatgttatgg aatacaattc aaaaggactt gccgccattg ataattgagg ttcagaaagt
     1681 gattaaccag gaatctttag gaaagtagtt aatgtataga aggattccga ataaataaat
     1741 cgtttattct tcctggattg cctattgaat gtggcctaca tcctcctgtg ggatgatgcc
     1801 tgtaaagcgg agcaactcta agttgaaatt tccacgaacg tgccgaacat accccgcact
     1861 acacgtatcc ttcagttaca ccccatgcct gcgtttttgc gcggtttccg agctcaattc
     1921 gcggagcgac aaaatgtctt ttaagacagc ccagaggggg atgtcactcg tttgcagtaa
     1981 tatccaatcg taaaccgaat aaaaagttcg gatagagctt ttgattgaac ataattgaac
     2041 ttaaccgttg taaaaagtac tttcgtggaa tataattgac ttattggtat gaaagcgatg
     2101 gggtggtaac cgttggaccg ggagatcctc aatctggagg aagcggccgg ccttttcggg
     2161 gtcagcgtta agaccttcat taaattactc aaggaagaga aggtccccgg ccggaagatt
     2221 ggccgcgaat ggcggtttag ccggggagcg ctcatcgact ggctctcaag gggagattcc
     2281 caaagttact cgtcgagcga gggtgaaacc agggaattct ttaatgaggt ggcgcccgat
     2341 tgggaggaac ttagccgggg ttactatgac gcttccatca aaaacaagct gattgaatgg
     2401 gagctgctcc ggccggatcg gatcttgctg gatctgggta cgggtgacgg ttatatcgcc
     2461 cgcttcgccg caccgtcggt cgggaaggtg ctggcgctgg atatctcggg ggcgatgctc
     2521 cgggagctgg agcgcaaggc acaggttgag gggctgcaaa atatcgaaac cgtccagggg
     2581 gatggctgcg atctaccatt cgccgataat agcgtggatc tggtcaccgc caatatgtat
     2641 ctgcaccata tcgaagaacc ggaagcggcc atctccgaga tcggacgggt gctccgcaag
     2701 gggggcgccg cctttcttgc cgattttgtc gaacacggtg acagggagat gatgacccgg
     2761 atgcacgacc tttggccggg ttttgcgccg gagacggtcc ggaagtggtt tat
//
LOCUS       DASVMB010000430         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_52284,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000430 DASVMB010000000
VERSION     DASVMB010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52284"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..2489
                     /locus_tag="VF531_12115"
     CDS             150..2489
                     /locus_tag="VF531_12115"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEX7714750.1"
                     /translation="MGLLDPAEWKAKWIAGNYVPKKDRYPSDYFRKTFTLNEQVKKAR
                     LYITACGLYEASINGHRVGTQCFTPGSTEYNKRLQYQTYDVAELLRDGMNAMAVVLGD
                     GWFRGQVGPFGSRRVFGAETKLLAQLEITTLSGEQITVVTDDSFMWSNVGAIRENDLQ
                     VGEVYDAAMALTGYDTPEFEDCSKFGPVKLTEYHTRLVCSNNVPVMEREHFKPKLIKT
                     PSGETVLDFGQNMAGYPAFSVSGPKGHAIILQMAEVLDEDGNFTVRNLVGNKLDFQRI
                     RYICSGNGKEYYKPKFAIQGFRYVLVKNWPGELNPDDFEAIAVYSAMEKTGDFTCSHP
                     LVNRLVENARWSMKSNFLEVPTDCPTRERAGWTGDAQLFFETGSYFMDVAPLYRKYLM
                     DIADCQGKDGKVPCIVPDVGSKVVSMLDGAVGWADAIILIPYRYWKIYGDKDIIKVNY
                     PAMKKYAGFIIDRATKNRLFSRFKKNPYRQYTYEVGFHWGEWLEPMENLSLKLLMKPK
                     REEATAYFSYTMSCLAEIADEIGETADAKQYRTCAEGAKKAYNYLYVKDNDIVADRPA
                     KYVRPLAFGLLPEEARKNVAASLLADTKARNYTIGTGFLSTPFILPVLTEFGYIDDAY
                     QMLEQEEIPGWLYQVKTGATTIWEKWDAIDKNGKVKFASHNHYSYGAVCNWLFRDVCG
                     IRLSDGDEYNYVIVPHPGGDLTYANASFRSIWGEVRSNWEIREDKVHYTIQIPANTTA
                     KLVLPVSADKGFQIIQGKDGFDDGQWTLGSGTYEFVTEK"
     gene            2564..3268
                     /locus_tag="VF531_12120"
     CDS             2564..3268
                     /locus_tag="VF531_12120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714751.1"
                     /translation="MDSNIIRLLSEEPVRNMAVLGFFKNYPLARSVQFGDSLVLLGES
                     DHLWGYVSSQSECELTRLFEEPVGTKFFASVEEWMKPVIAKNFEVEWELSTDRYLLPA
                     NHPIDPPPRMTCTLEVPFAEYIFDHSDYKAFTSVEYLRDRIARGISAGVIENGTLAAW
                     ALTHDDNSLGCLHVLPEFRRKGFAKAIMGSLIIQKRKAGEPVFLNVEPSNISSRNLVT
                     GMGFVLDRKISWIKLK"
     gene            3400..3555
                     /locus_tag="VF531_12125"
     CDS             3400..3555
                     /locus_tag="VF531_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714752.1"
                     /translation="MKNFHLSDYANDNVSVKLSFERDFMSYTMKDLAKLAGVSTNTVS
                     RACRFLR"
BASE COUNT         1070 a          737 c          983 g         1052 t
ORIGIN      
        1 gggaaagtgg aaaagttgca agtgaccgga gtatccgcat ccaatatcaa gggccaaagc
       61 tctttagtcg tgaaattatc ttttggaaag ttaaaatatg ggatgaagcc ggggaagaat
      121 cctagtggag tgaaacggcc agttttgaga tgggactact tgatccggcg gagtggaagg
      181 caaagtggat cgcaggtaac tatgttccta aaaaagatcg ttatccgtca gactatttcc
      241 gcaagacctt cactttaaat gagcaagtta aaaaagcaag attatatatc actgcttgtg
      301 gtctatacga ggcaagtatc aatggacacc gagtcgggac tcagtgtttc acccccggca
      361 gtacggagta taacaagcgg ctccaatatc agacttatga tgtagccgag ttgctcaggg
      421 acgggatgaa tgcgatggcg gttgttttgg gcgacggctg gtttcggggg caggtgggtc
      481 cttttggatc gagacgggtg tttggggctg agacaaagct actcgcccag ttggaaatca
      541 caactctttc cggagaacag atcacggttg tgacggatga cagttttatg tggagtaatg
      601 taggagcaat ccgggaaaat gatttacaag ttggtgaagt atatgatgcc gccatggcat
      661 taaccggcta tgatacgcct gaatttgaag actgttcgaa atttggaccc gtaaaactta
      721 cggaatatca tacccgtttg gtttgctcca ataatgttcc ggttatggaa agggaacatt
      781 ttaaacccaa attgataaag actccgagtg gagaaacggt tttggatttc ggacaaaaca
      841 tggcagggta tccggccttt agcgtctcag gaccgaaagg acatgccatt attttacaga
      901 tggcagaggt attggatgaa gacggcaatt ttactgtaag gaacctggtt ggtaataaat
      961 tggattttca aagaattcgc tacatttgca gtggaaatgg aaaggaatat tacaaaccaa
     1021 aatttgcgat acagggattc cgttatgtgc tggtcaaaaa ttggcccggg gaattgaatc
     1081 cggatgattt tgaagcgata gccgtatact ccgccatgga aaagacaggt gattttacct
     1141 gtagtcatcc gctcgtcaac cgtctggtgg aaaatgcccg ctggagtatg aaatctaatt
     1201 ttttagaggt tcctaccgat tgccccacca gagaacgtgc cggttggacc ggggatgccc
     1261 agttgttttt tgagaccggc agctatttca tggatgtcgc tccgctctat cgcaaatatc
     1321 taatggatat tgccgactgt caggggaaag acgggaaagt cccctgtatc gtcccggatg
     1381 ttggttccaa ggtagtctcc atgcttgatg gggctgtggg ctgggcagac gccatcatat
     1441 taatcccgta tcgatattgg aagatttatg gcgataagga tattattaag gttaactatc
     1501 cggcgatgaa aaaatatgcc gggtttatca tcgatcgtgc tacaaaaaat aggttattca
     1561 gccgttttaa gaaaaatcca taccggcaat atacttatga agttgggttt cattggggtg
     1621 aatggctaga acccatggaa aatcttagcc ttaaactgct gatgaaacca aaacgggaag
     1681 aggcaacggc ttatttttcc tataccatgt cctgtttggc ggagatagcg gatgaaattg
     1741 gtgaaactgc cgatgcgaaa caataccgaa catgtgcgga gggtgcaaag aaagcatata
     1801 attatcttta tgtaaaggat aatgacattg ttgcggatag acctgcaaaa tatgttcgtc
     1861 cattggcttt tggccttttg ccggaagaag ctcggaaaaa tgttgcagct agtctgcttg
     1921 cggataccaa agccaggaat tacacgatcg gaaccggatt tttatctaca cccttcatac
     1981 tgccggtatt gaccgagttc ggatacattg atgacgccta tcaaatgtta gagcaagaag
     2041 agattcccgg ttggctctac caagtcaaga caggagcaac aacaatatgg gaaaaatggg
     2101 atgccatcga caaaaacgga aaagttaaat ttgcctccca taatcattat tcgtacggtg
     2161 cagtttgcaa ttggttattt cgggatgtct gcggcatccg tttgagtgat ggagatgaat
     2221 acaactatgt cattgtcccg catccgggag gagatttaac ttacgcaaat gcctcttttc
     2281 ggagtatctg gggtgaggtc cgcagcaact gggagatcag ggaggataaa gttcattata
     2341 ccattcaaat tccggccaat actacggcta aactggtcct gcctgtttcg gcggacaagg
     2401 gttttcaaat tattcaaggt aaagatggat ttgatgatgg acaatggacg ctgggctccg
     2461 gtacttatga atttgtcaca gagaagtaaa gcccattcac attatatcgg ttaaaaaggc
     2521 tcccggaact ctccgtggta tcgaagggaa gagaggaaac aatttggact ccaatatcat
     2581 tcgcctgtta tcggaagaac cggtacgtaa catggctgta ttaggttttt ttaaaaacta
     2641 tccattagcg agatctgttc aatttggtga tagtcttgtt ttattggggg aaagcgacca
     2701 tttgtgggga tatgtctcca gccaaagtga gtgtgagtta acccggcttt ttgaagaacc
     2761 ggtcggcacc aaattctttg catccgttga ggagtggatg aaaccggtta tcgccaaaaa
     2821 tttcgaagtt gaatgggaat tatcgaccga ccggtacttg cttcccgcga accatccgat
     2881 tgaccctccg ccacgaatga cttgtaccct ggaagtacca tttgcggagt atatcttcga
     2941 tcactcggac tacaaggcgt ttacttccgt ggagtatctt cgcgatcgga ttgcccgggg
     3001 aatatcggca ggagtcatag agaacggcac gctggccgct tgggccctta cccatgacga
     3061 caactccctc ggttgtcttc atgtacttcc ggaattcaga agaaagggtt ttgccaaggc
     3121 tatcatggga tctttgatca ttcagaagcg taaagcgggt gaaccggtgt ttttgaatgt
     3181 ggaaccttcc aacatcagtt cccgcaacct cgttaccgga atgggatttg tcttggatcg
     3241 gaaaatcagc tggatcaagt taaaataacc gacccgaatc atcgtcttgt tgaacaccat
     3301 cgaaaagatg gtgttttttt atggttagtt atgggctgat agagagagcg tggagttctt
     3361 aaaataattt ttacaaggag gaaggattat gaataattga tgaagaattt tcatctaagc
     3421 gattacgcga acgataacgt gagtgttaaa ctatcgtttg agagggattt catgagttat
     3481 acaatgaagg atttggcgaa actggccggg gtttcgacga atacggtctc ccgggcctgt
     3541 cgtttcttgc ggtaatactc ggactcgtcg tcaacctatt aggtttgaaa ttacccgttt
     3601 tggtgatgga accgctagag attatagggg gcgtggcgtt gccaccggta atgcttccct
     3661 gattttcagc atatcgttat atgcgtaacg cagcttactt cactttggaa ttggaaatta
     3721 caaattccag agtgaagttt tttttacgcc atgtttattt cgtgtatttg gtgcttttcg
     3781 tggttaaaag tttctatcgg taagcctatt ttattcacaa cgaaacacac aaaagaaacg
     3841 aa
//
LOCUS       DASVMB010000431         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_128031,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000431 DASVMB010000000
VERSION     DASVMB010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128031"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2381
                     /locus_tag="VF531_12130"
     CDS             <1..2381
                     /locus_tag="VF531_12130"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX7714753.1"
                     /translation="EEVPSMRYGFFDDSAREYVIERPDTPMSWANYLGTAEYCGLISN
                     NASGYGFYKSPKSARLLRFRFNSIPMDRPGRYVYIRDNATGDYWSASWQPVGKPQDQY
                     KTTCRHGLGYTRFDSEYQEIRSHFRVFVPIDKPIEFWELELENCSDRERDFSLFTYAE
                     WCFWDMNQDLSNFQYILYTCRMGYENDIVDYSIHLWPSNEPKAFMASTLPVVSFDTDR
                     EEFIGRYRHEGNPVAVEKGKCSNSIAVGGNPCASVQNRITLKPGEKKYALYIVGVGDA
                     KSFGVECKRKYSDRAQVTAEFDKVQAYWKDRLGKFTCITPSAIFNSMANIWNPYQCHT
                     TFNWSRSASFNEAGGRDGLGYRDSNQDTLGVMHSIPALCKSKLTDLLKGQISNGAAMH
                     GVQPLTWTQGPQNVPPEAQIYSDDHLWLLLSIPAYLKETGDMVFLDTAVPYADRGEDT
                     VYRHLQVALEFSWTKRGPHGLLLGMAADWNDCINLKGKGESIWSTFLYYRGLNEFITL
                     AERIGKGSDAEHFAEYRREIKASLDKFAWDGQWYLRGYLDSGKKLGGAQSEQSKIFIN
                     SQTWSVISEAADRDKGIQAMDSLKQYLATEHGIVKNYPAYREEDLEVGAITSFPPGLK
                     ENAGIFCHANTWAVIAECLLGRGDRAFEYYLSFLPAAKNDDADRYTMEPYVYSQFITG
                     KEHPYKFGRARNSWLTGTASWSYVALSQYILGVRSDYDSLVIDPCIPHDWEGYQVSRE
                     YRGKTFEITIKNPHKVSSGVAGMVVNGKEINGNKIPIDQMEEKNRVEIILGK"
     gene            2445..3887
                     /locus_tag="VF531_12135"
     CDS             2445..3887
                     /locus_tag="VF531_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714754.1"
                     /translation="MKAFQLHMGPAKCAVDLGDGSERGEYVNQDYILAKLGRPHRSIN
                     LMYCYYPLDQGWPARANTLGSNSEVSFAWDFPYDDYFPYLGGVGGGTEAEPFISMRDV
                     RRHGQDVTLTLTIDCAVPDEYLVKIANELKPFGRMLLRINHEATGNWFAFNKRYSYRE
                     VADFFVRFHQIIKREAPQIRTILCIGGGSKDGKTMPYEQEFEKAIQAADIWSGDYYLA
                     LNWGWPYTIAEPGGKTHKRSIVKDVYEENRFSFERFTLRNGGLAKPLVISELNADGDV
                     TGPYDQAEMVREFYSTIKKDSATWLSGITFYQFQDRGRLGLELEDPNNPNIGIEQPLL
                     AAYRELIHDPYFLPEFTLEDEITFPIRLRWGSAEDSEGLAISLNFETRPVFCEVYFPE
                     PELNLIFELNGEWFYKKSGVKTIDLMPAFVKKTLAKKELIFKMFAPPASGENDPSQGA
                     DWEANYYTEVHQLPRFRIRYRPTENGFYIP"
     gene            complement(3901..>4484)
                     /locus_tag="VF531_12140"
     CDS             complement(3901..>4484)
                     /locus_tag="VF531_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HEX7714755.1"
                     /translation="KIPLSSKLIICFFSVFYSGGALLLGKTLAKLIHPDWSGWIGSVI
                     LILMGVWIIVQVLCKQDSKPRELIPVRNETKTLFKIVIKSLGITIHVIRNPAEMDMDY
                     SGSIDSRESFLLGLALSIDAIGAGVGSGLIGITSWVIPILVGVFQLCFLYFGCYVGER
                     THVAMKINQKFLSLSPGFLLILMAIIRMVQLPK"
BASE COUNT         1140 a         1060 c         1173 g         1111 t
ORIGIN      
        1 aagaggaggt tccatccatg cgttatggtt tttttgacga ctcagcacgg gagtatgtaa
       61 tcgaaagacc cgataccccc atgtcgtggg ctaactacct gggaaccgct gaatactgcg
      121 ggctcatctc caacaacgcc agcggatacg gattttataa atcgcctaaa tctgcccggt
      181 tattacgttt tcggttcaat agcattccca tggatcgtcc cggccgttac gtctatatcc
      241 gggataacgc caccggagat tattggtccg catcgtggca gccggtgggc aagcctcagg
      301 accagtacaa aaccacctgc cgtcatggtt tgggatatac ccgtttcgat agcgaatatc
      361 aagagattcg ctcccatttc cgggtatttg tccccatcga caagcctatc gagttttggg
      421 aattggagct tgagaactgt tcggatcggg agcgtgattt ctcccttttt acatacgccg
      481 agtggtgttt ttgggatatg aaccaagacc tcagcaattt ccagtacatt ttatatacct
      541 gcaggatggg ttatgaaaat gatatcgtag attattcgat ccatctttgg ccttccaatg
      601 aacccaaggc ctttatggcg tcgaccttac cggtggtgag ctttgatacc gatcgggagg
      661 aattcatcgg ccgttatcgc catgaaggca atccggtcgc ggtagagaag gggaaatgct
      721 ccaattcgat tgccgtggga gggaatcctt gcgcttcggt tcaaaaccgg atcaccctca
      781 agccgggtga gaaaaaatat gccctctata tcgtcggggt gggagacgcc aagagttttg
      841 gagtcgagtg caaacggaaa tattccgacc gggctcaggt cacggcggag ttcgacaagg
      901 tgcaggctta ctggaaagac cggctcggga agtttacttg cattactcca tccgccattt
      961 tcaattctat ggcgaacatt tggaatccct accagtgcca taccaccttc aactggtccc
     1021 gttccgcttc attcaatgag gccggcggca gggacggttt aggttaccgt gactccaacc
     1081 aggataccct gggggtgatg cattccatcc ctgcactgtg caaatctaaa ctgactgatc
     1141 ttttaaaagg ccagatttca aatggcgctg ccatgcacgg ggttcagccc ctcacctgga
     1201 cccagggacc gcagaatgtt cctccggagg cgcaaatcta ctcggatgat catctttggt
     1261 tgctcttatc cataccggct tatctaaaag agaccgggga catggtattt ttggatacgg
     1321 cagttcccta tgccgatcgg ggcgaggata ccgtataccg gcatcttcag gtggctttgg
     1381 agttttcatg gaccaaacgc ggcccccacg gactgttgtt gggaatggcc gccgactgga
     1441 acgattgcat caacctcaag ggaaaaggcg agagtatctg gagcactttc ttgtactatc
     1501 gcgggcttaa tgaattcatc acgctggcgg aacgaatcgg taagggtagt gatgcggagc
     1561 atttcgccga ataccgccgg gaaatcaagg caagtctcga taagttcgcc tgggacggcc
     1621 agtggtatct ccggggctat ctggacagcg gtaaaaagtt aggtggagcg caaagcgagc
     1681 aatccaagat cttcatcaac agccagacct ggtcggttat ttccgaagcc gccgaccggg
     1741 ataagggaat tcaggcgatg gacagcctga agcagtattt ggccaccgag cacgggattg
     1801 tcaagaatta cccggcgtat cgcgaagagg acttagaggt cggggccatt acctcttttc
     1861 caccggggtt gaaggaaaat gccggtatat tttgtcatgc caatacatgg gcagttattg
     1921 ccgaatgcct gttgggacgg ggcgatcgtg cctttgaata ttacctttcg tttcttccgg
     1981 cagctaagaa tgacgatgca gatcggtata ccatggagcc ttatgtctat tcgcaattta
     2041 ttacggggaa agaacacccc tataaatttg gccgggctcg aaattcttgg ttgaccggaa
     2101 ccgcctcttg gagttacgtg gcgctttccc aatacatcct gggagtccgc tccgattatg
     2161 acagtttagt tattgatccc tgcattcccc acgattggga aggatatcaa gtaagccgag
     2221 aataccgggg gaagactttt gagatcacaa tcaagaatcc acataaggtc agcagcggtg
     2281 tggcaggcat ggtggtaaac ggtaaagaaa taaacgggaa taagattccc attgatcaaa
     2341 tggaggaaaa gaaccgggtt gaaatcatcc tgggaaaata attatcgtag ggaacacgca
     2401 tgcgtgttcc ctacatatgc attatcgaag gatcggtgaa tatcatgaaa gcctttcaat
     2461 tacatatggg tcctgccaag tgtgccgttg atctgggcga cggaagtgaa cgtggcgaat
     2521 acgttaatca ggattatatc ctcgccaaac tgggccggcc gcaccggagt atcaatttaa
     2581 tgtactgtta ttatcctttg gatcaaggct ggccggctcg tgccaatact ttgggtagta
     2641 attcggaggt ctcgtttgcc tgggattttc cttatgatga ttactttcct tatttaggag
     2701 gggtcggagg cggcacagag gctgagcctt ttatctccat gcgtgatgta cggcgccatg
     2761 gtcaggatgt cacgctgacc ctcaccatcg actgcgctgt tccggatgaa tatttggtga
     2821 aaattgccaa tgagctgaaa ccctttggca ggatgctgct tcggatcaac catgaagcca
     2881 ccggtaactg gtttgccttt aataaacgat acagttaccg ggaggtggcc gattttttcg
     2941 tccggtttca ccaaatcatc aaacgggagg caccccagat ccggacgata ctatgtatcg
     3001 gcggcgggag caaagatggg aagaccatgc cctatgagca ggaattcgaa aaggcgattc
     3061 aggccgcgga tatctggtcg ggcgattatt acctcgcgtt aaactggggg tggccttata
     3121 ccatcgccga accgggtggt aaaacccata aacgttccat cgtgaaggat gtctatgagg
     3181 aaaaccgctt tagtttcgag cgttttactc tccgcaatgg aggtttggcc aaacccctgg
     3241 tcatatcgga gctcaatgcc gatggggatg tgacgggccc atacgatcag gcggaaatgg
     3301 tgagggagtt ttattccacc atcaagaagg attccgccac ctggctttcg ggaatcacgt
     3361 tttaccaatt ccaggatcgg ggcaggttgg gattggaatt agaggatccc aacaacccaa
     3421 atattgggat tgaacagccg ttactggcgg cctacaggga attaatccac gatccctatt
     3481 tccttccgga atttaccctt gaggatgaaa tcacgtttcc gattaggcta cgctggggga
     3541 gcgccgagga ctcggaagga ttggccattt ctttgaattt tgagactcgt ccggtcttct
     3601 gtgaggtcta tttccctgaa ccggaactga accttatttt tgagctaaac ggagagtggt
     3661 tttataaaaa atccggagta aaaaccatcg atctgatgcc cgctttcgta aagaaaacgt
     3721 tggcaaagaa ggagcttatc tttaaaatgt tcgccccgcc ggcctcgggc gagaatgatc
     3781 cttcccaggg ggcggactgg gaagcaaatt actataccga ggtccatcaa ttaccccggt
     3841 ttcgcatcag gtaccgcccg acagagaatg gattctatat tccgtaaaat ccggatcttc
     3901 ttatttcggt aactgaacca tgcggatgat ggccattaag attaatagga atcccggtga
     3961 caatgatagg aatttttggt taatcttcat ggcgacatgg gtccgttcac cgacatagca
     4021 tccgaaatag agaaaacata attggaaaac gccgaccaat atggggatca cccaggaagt
     4081 aataccgatg agtccgctgc cgactcctgc tccgattgcg tcaattgaga gcgccagccc
     4141 aagtaggaat gattcgcggg aatcaatact cccggagtaa tccatatcca tctctgcagg
     4201 attacggatc acatgaatcg tgatccccaa tgatttgatc acgatcttga acagtgtttt
     4261 ggtttcattc cggaccggga tgagctcccg aggtttcgaa tcttgtttac acaagacctg
     4321 aacgatgatc cagaccccca tcagaatcag aatgactgat ccgatccatc ccgaccaatc
     4381 cggatgaatc agcttggcga gcgtctttcc caaaagtaat gccccgcccg aataaaacac
     4441 ggaaaagaaa cagatgatta gtttggaact caaaggaatt ttga
//
LOCUS       DASVMB010000432         9591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_422281,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000432 DASVMB010000000
VERSION     DASVMB010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422281"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VF531_12145"
     CDS             complement(<1..198)
                     /locus_tag="VF531_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7714756.1"
                     /translation="MEIRQIGKSKLQLAVLGLGCWPFGGGKYWGGQDQKDVNDVVKAA
                     VDLGINYFDTAEAYNDGESERS"
     gene            complement(397..1377)
                     /locus_tag="VF531_12150"
     CDS             complement(397..1377)
                     /locus_tag="VF531_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7714757.1"
                     /translation="MMSGQVKWGVLGYASIARESFIPALLKAGNSKFCALASRSPEKL
                     RLCGERFNYPEKTYTDYEQLLDDPEIEAVYIPFPNSLHKEWTIKAARKGKHVLCEKPL
                     ALDGEDCRQMIEECRRNNVKLMEGFMYRYSDRIRKVQKAINEDKIGRVKYIHSYFFSL
                     GSRASGDRIDPNLGGGSLYDLGCYPVNFVGMITGAEPVSIAAEAVPINGVDQILSAVM
                     RYETGVIANIHCGWINEFRSLGTTIMGTKGTLEIPEPFWGNPGSITVTTPDGKEEIPV
                     AESDRFRAEIEDFAGAILEDRPPMLDPGEAIRNQKVIKEILIKAGFTDRF"
     gene            complement(1408..2277)
                     /locus_tag="VF531_12155"
     CDS             complement(1408..2277)
                     /locus_tag="VF531_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714758.1"
                     /translation="MKIDSAAIEQMMLKPKKQFSYGWIWTLLLVIGALFMVFPFIWMV
                     LTSLKTPAEIQQVPLKFWPDNLFNLQNYIDLFKRQPFHLYVWNTIVVSGISTFTSVIV
                     SAMAGYGFAKYHFPLKEFWFFCILALLMIPFQAIVIPLYQWVVQFGLINTYVGLMLPQ
                     FVSAFGVFLMREAVTGIPNDYIDSARLDGSSEIGIFFRIILPSITPSLAALTIIKFLW
                     TWNEFFWPLVATNSDAMKVITLGLASFNNQYFVEYHLATAASVVSIIPILILFLAFQR
                     WMVKAVVMSGIKG"
     gene            complement(2305..3225)
                     /locus_tag="VF531_12160"
     CDS             complement(2305..3225)
                     /locus_tag="VF531_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714759.1"
                     /translation="MELHDKGTIKGLFLGQTFSDKRARIGYAFVLPAVIYFLLVYFYP
                     LFQSIQMSFFRGGFGEAPKFVGFASYENVFTDPMFWLTVRNTLYFVILSVPATVFLAL
                     MVAILLNRIKNRRFRDILSATYFLPLVTSLVAAALIWGWIYQPIYGLANYLLAIIGLP
                     AQKWLSSTTQVMPSLAFINIWLRVGFDTIIFLAALQSIPDELMEAAKIDGANALRTFR
                     HITLPLLNPQIVMVSILELITAVKVFDQVYATTVGGPANASRVIMMYLYNMSFQWFKF
                     SEASVVAIFIFISLLIISVLQWVFLRKSAY"
     gene            complement(3310..4587)
                     /locus_tag="VF531_12165"
     CDS             complement(3310..4587)
                     /locus_tag="VF531_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7714760.1"
                     /translation="MTSKKRFLLIGLALLIIASLATVSAAPKKVKLVYWTHWEQNPKF
                     NAYYAEAGKEFAKLHPECEGVEVVTVPYSGYEAKYLAAFMARTGAPDFFNGAAHDWAG
                     KYQFADKMPANIAKRVNTEAANTAVKTGIYNGVRYGFPVEGGNFQYMFINTEMFTEAG
                     LDPNKPPMTLTELLDDAKKLTKYDASGKVIRSGFGLRYAGDQTGITDKFLPVLHAFGG
                     LMVDPKYKKSLGVVNSAAGIEALNFYGDLVNKYKVSSLEVGNPETAFGKGLAAIIFRE
                     SWLPGWLASNAPNIKYKVYALPSQKVAPGPGALFGWSDLVYKNSPNKKLAWEFLNFMW
                     SKENDLARATAQGIMPVFNSNFNTDFVKNRPDYTAVMDMAKRQPAPTYYSPKMNEVAA
                     AYGDAVLDVVYGRKDAKAALNAAAVKIDKILKQ"
     gene            complement(4888..5667)
                     /locus_tag="VF531_12170"
     CDS             complement(4888..5667)
                     /locus_tag="VF531_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7714761.1"
                     /translation="MGSLQGKKVLITGGANGLGGAISLELAKRGASIAVHWFQHRETQ
                     NTAQDLAELREKVKAFGTKMVDVSGDLTDEATVKNVVQDAAEGLGGLDILVNNVGDII
                     GRQTLEGMELSFYQKVMDVNLKTMFLVTREALPYLFQANGAAIVNLASLAGRKGGHGG
                     SLIYGTAKGAVITLTRSLSTEIGPKGVRVNCVAPGLILGTYFHQTHTTDESARKTISE
                     IPLGRAGCPQDIADGVAFLASQYDGFITGVTLDINGGVYMA"
     gene            complement(5686..6114)
                     /locus_tag="VF531_12175"
     CDS             complement(5686..6114)
                     /locus_tag="VF531_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HEX7714762.1"
                     /translation="MLKNIPAIISPELMKTMMEMGHSDEIVLADANFPAATCAKRLIR
                     CDGLVLPQLLEVMLALFPLDKTVKYSAVLMSVTAEETAPPIWEEYRKIIRQAESDFRE
                     FEHIERYQYYERAKNAFAVVITGDTSFRANILLKKGVVRP"
     gene            6399..8399
                     /locus_tag="VF531_12180"
     CDS             6399..8399
                     /locus_tag="VF531_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7714763.1"
                     /translation="MEKDRIQIDGKEYPLYRCHTLIIGSGAAALNCACHLSDLGVNDL
                     LIVTEQLGGGTSNNSGSDKQTYYKMSLFGEDQDSVYEMARALFSGGSMHGDIALVEAS
                     LSAREFFHLVEIGVPFPHNQFGGYVGYKTDHDPKQRASSAGPWTSNQMYQKLLIQAQQ
                     KQLPIMDGYEVISLLTAPDDKGPRAIGAIALDKAKLHSGTGSLLVLQAENIVMGTGGP
                     GGLYQTSVFPKGHLGSTGIALEAGATAVNLTEWQYGISSTQFRWSVSGTYQQVIPRYI
                     STNQDGGDPREFLNDYFPTPGKLGTATFLKGYQWPFDPRKVKNWGSSLIDILVYQENV
                     LKKRRVFMDFRNNPSFDQKSGEFCFDQLEPEAFQYLKKSNGLFGTPIERLAKINPLAI
                     ELYAQHGIDLHHEPLEIAVCAQHNNGGLKGDIWWQSNLRHLFPVGEVNGTHGVYRPGG
                     SALNSGQVGGCRAAEYIAARYSGYTMAGEQFVMLSQNQVTDKLHLMNQVLGNNEKSGQ
                     DLASFRKEFQERMSHVAAHIRELTAVRAAVKEAEAQWQRINSGEVRLQDHSELTAFFQ
                     NRQLCLTQLAVLKSIEAYLEKGGGSRGSYLVLDRNGERISDQMDTSWNFRLENEELRR
                     YTLECNYCSNEFSLSWVPVRPIPTDDFWFENVWRAYRDKEIY"
     gene            8448..>9591
                     /locus_tag="VF531_12185"
     CDS             8448..>9591
                     /locus_tag="VF531_12185"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7714764.1"
                     /translation="MIRNWNEESLDHRNALLYAMGVSPEDAKRPVIGIINSWNEMNPG
                     HFDFKDVIQELKDEIYNAGGLARELPVSGICDGICSNTPGDRYTLPARDLVASEVETT
                     AELNMLEGMILLGSCDKVVPGMLMGIMRVNIPAVMFTGGYMEPGFHNGKMITLTHTKQ
                     AYAAYIAGDISQKEYKDIVSEACPTPGICPFMGTANTMCAAAEILGFSPAGNASVKAQ
                     SPEWHEMARQAARKIVELVKKEIRPLDVVNIDNFENLVRYMMATGGSTNSILHIPAIA
                     RQAGMDITPEFFDKISRDVPVINAIYPNHKTYTMVDFSEAGGLGAVLKELAAARKINT
                     SARGMFGTVGEQIAKAQNKNKQVIHEVKNPINAQGGLAVLKGNIATE"
BASE COUNT         2588 a         2373 c         2238 g         2392 t
ORIGIN      
        1 ggatctctca ctctcgccgt cattataggc ttcggcggtg tcaaaatagt ttatccctaa
       61 atctaccgcc gcttttacca catcattcac atctttttga tcctggccgc cccagtactt
      121 tccgccgcca aagggccaac aacccaaccc caataccgcg agctgcaact tggattttcc
      181 aatctgccta atctccatcc gttttcactc ctatttttta aaaagcttaa ggacacgaaa
      241 gcctgccatc gaaatcggtc ttattttcag gttgaagcgg gaactttacc ggcgcccggt
      301 cacggttcga ccggtagatt gcggtaaaga tctcgaccgt cttggggcct tcctcttgcc
      361 atggtgacct ttacatcctt attgggattg gaaatgctaa aatctatcag taaatcccgc
      421 tttgattaat atctccttga taactttctg gttacgaatt gcctccccgg gatctaacat
      481 cggaggacgg tcttccagga ttgcgccggc aaaatcttca atctcggcgc ggaaccggtc
      541 ggactccgcg acgggaatct cttcctttcc atcgggggtt gtaacggtaa tcgaaccggg
      601 attcccccaa aagggttccg gaatctccaa agtccccttg gtccccataa tcgtcgtccc
      661 caaactccgg aactcattga tccagccgca atgaatattc gcaatcacac cggtttcgta
      721 tcgcatcaca gcggatagta tttgatcaac cccattgatg ggaacggcct cagcggcaat
      781 ggaaaccggt tccgctcccg taatcattcc cacaaaattc acaggatagc atcccagatc
      841 atagagtgag ccgccgccca gattcggatc gatccggtct ccgctcgccc ggctccccaa
      901 ggagaaaaag taggaatgaa tatatttcac ccggccgatt ttgtcctcgt ttatagcctt
      961 ttggactttg cggatccgat cggaatagcg gtacataaaa ccttccatga gctttacgtt
     1021 attgcgccgg cattcctcga tcatttggcg gcaatcctcg ccatcaaggg ccagcggttt
     1081 ttcgcatagc acatgcttcc ctttccgggc cgctttgatg gtccactctt tatggagtga
     1141 attgggaaaa gggatataga ccgcttcaat ctccggatca tccagtaatt gttcgtaatc
     1201 ggtataggtt ttttccggat aattgaaacg ctcgccgcat agacgcagtt tttccgggga
     1261 gcgcgatgcc aaagcgcaaa atttggaatt accggctttt aataaagctg ggataaatga
     1321 ttcccgcgca atggatgcat aacccagaac accccatttt acttgaccag acatcatgcg
     1381 catccaccct ttcccgactg ataatattta gcctttgata ccgctcatta caactgcctt
     1441 aaccatccag cgttggaagg ccaggaacag tatgagaata ggaataatgc tgaccaccga
     1501 agccgccgta gccagatgat attccacaaa atactggttg ttgaacgatg ccaggcccag
     1561 ggtaatcacc ttcattgcat cgctgttggt ggcaaccagc ggccagaaga attcattcca
     1621 ggtccataag aacttaataa tggttaatgc cgccaatgac ggcgtaatgg aaggcagaat
     1681 aatccggaaa aagatgccga tctccgagga accgtcaagc cgggccgaat caatataatc
     1741 gttggggatt ccggtaaccg cctcgcgcat cagaaatact ccaaaggcac tgacaaactg
     1801 tggcagcatc aagcccacat aggtattgat taaaccaaat tgcaccaccc actgatacaa
     1861 cgggatgacg atggcttgaa acgggatcat gagtaatgcc agaatgcaga agaaccagaa
     1921 ttcctttaag ggaaaatgat atttggcaaa accgtacccg gccatagccg aaacaattac
     1981 acttgtaaat gtgctgatgc cggagacaac gatggtattc caaacataca ggtgaaatgg
     2041 ttgccgctta aagagatcaa tatagttttg cagattgaat agattatccg gccagaattt
     2101 cagcggaact tgctgaatct cggcaggcgt ctttaatgaa gtcaaaacca tccagataaa
     2161 cggaaacacc ataaacaaag ccccgattac aagcagcagg gtccagatcc agccgtaact
     2221 aaactgcttt ttgggcttaa gcatcatttg ttcgattgca gcgctgtcta ttttcatatt
     2281 ctccccacct ttatcaggta aactttaata ggccgacttg cgtaagaata cccattggag
     2341 cacactaatg atgagtaacg agatgaatat aaagatcgca actaccgagg cttcactaaa
     2401 cttgaaccat tgaaacgaca tattgtaaag atacatcata atgacccggc tggcatttgc
     2461 cggcccgcct accgtagtgg cataaacctg gtcgaaaact ttaacggcgg taatcaactc
     2521 taatattgaa accatcacaa tctgtggatt tagcaggggt aaggtgatat gccggaaagt
     2581 cctcagagca ttggcgccgt caatttttgc ggcttccatt aattcgtcgg ggatgctttg
     2641 cagggcggcc aagaaaataa tggtatcaaa accgacacgc aaccaaatat tgataaatgc
     2701 caaagagggc atgacttgcg tcgttgaact caaccatttc tgggccggta aaccgatgat
     2761 tgccaataaa tagttggcca atccgtatat cggttgataa atccatcccc aaatcagtgc
     2821 cgcggccacc aaggaggtga ctaagggcag aaagtaggtg gcgctcagga tatcacgaaa
     2881 tcttctattt ttaatccgat tcaataaaat cgctaccatt aacgccagga aaacagtagc
     2941 cggtacggat aagattacaa aataaagtgt gtttcttaca gtgagccaga acatcgggtc
     3001 cgtaaagaca ttctcataag aggcaaaacc gacaaattta ggagcctctc caaagcctcc
     3061 cctaaaaaac gacatttgga tggattgaaa cagaggataa aaatagacga gcaaaaaata
     3121 gattactgcc ggtaaaacga acgcgtaacc aattctggcc cttttatcgg agaaggtttg
     3181 acccaaaaat aagcccttga tagtcccttt atcgtgaagc tccattttaa cacctcatta
     3241 actagaactt tcgtagtatt tggaggaata aagggcggag aggctccgcc cttatggata
     3301 catgaatgct tattgtttca atattttatc aatcttcacc gctgccgcat ttaaggcggc
     3361 tttggcgtct tttcggccgt aaacaacatc cagaaccgca tctccgtaag cagctgcaac
     3421 ttcattcatt ttcggagagt aataagtcgg agccggttgg cgttttgcca tatccatgac
     3481 tgcagtgtag tccggacggt ttttaacaaa gtccgtattg aaattcgaat tgaataccgg
     3541 cataatccct tgggcagtgg cccgggccag gtcattctcc ttggaccaca tgaaatttaa
     3601 gaattcccag gccagttttt tattgggact atttttatac accaaatcgg accatccgaa
     3661 cagcgctccg gggccggggg caaccttttg agacggtaat gcgtatactt tatacttgat
     3721 attgggagca ttggaagcca accacccggg aagccaggac tcacggaaaa taatggcggc
     3781 taaacctttg ccgaaggccg tttcgggatt tccaacttca aggctggata ccttgtattt
     3841 atttacgaga tcaccgtaaa aattaagggc ctcaatgccg gccgcgctat tgaccacccc
     3901 aaggcttttc ttatatttgg ggtcaaccat caaaccgcca aaagcatgga ggacgggaag
     3961 aaatttgtcg gtaataccgg tctgatcacc ggcgtaacga agtccaaaac cggaacgaat
     4021 gactttgccg cttgcatcat atttggttaa tttcttggcg tcatccaata gctcggttaa
     4081 agtcatcggc ggtttgttgg gatccagtcc tgcttcggta aacatctcgg tattgatgaa
     4141 catatactgg aaattaccgc cttcaaccgg gaaaccataa cgcacaccat tatatatacc
     4201 agtcttcaca gccgtattgg cagcttccgt gttaaccctc ttggcgatat ttgccggcat
     4261 cttatcggca aactgatatt tacccgccca gtcatgagcg gcgccattga agaagtccgg
     4321 ggctccagtc ctagccataa aggctgctaa atatttcgcc tcataacccg aataaggaac
     4381 agttactacc tcaacccctt cgcactccgg atgaagcttg gcaaattcct taccggcctc
     4441 ggcgtaatat gcgttgaact tcggattctg ttcccagtga gtccaataaa ccagcttcac
     4501 cttttttggt gcggcggata cagttgcaag cgatgcgatg attaacaatg ccaatccaat
     4561 cagtaaaaac cgcttcttac tcgtcatttc ttatcctccc tttttttgtt aaacttggca
     4621 accaagacga atcctcctat cacccccttc aacagccgca tggatcttct caagccaaaa
     4681 aaatttccat cccggctaac tcaatattgg cattatataa taaagtaaga ttgaaacggt
     4741 tacgaaaatg attactacaa ggtgatataa aaaaacggca atgaattatc tacaaccagt
     4801 atacaacctg ttaatcaaac tttatcacct tggattccaa aatgcaataa tctggttaaa
     4861 aatttcttga tgcccgatca atagatgtca agccatgtaa accccgccat tgatatccag
     4921 ggtcaccccg gtgataaaac catcatactg agaagcaagg aatgccacac catcggcgat
     4981 atcttgcgga catccggccc ggcccaaggg aatttcagag atggtcttgc gggcggactc
     5041 atcggtggta tgggtctggt gaaaataagt tcccagaatg agcccgggtg cgacgcagtt
     5101 tactctcacg ccttttggcc cgatttcggt cgacagcgaa cgggtaaggg taattaccgc
     5161 gcctttggca gtaccgtaaa tcaaggaacc gccgtgcccg cctttacgcc cggcgagcga
     5221 cgccaggttg acgattgccg cgccattggc ttgaaagagg tatggcagtg cttcgcgggt
     5281 cactaaaaac attgttttga gattcacatc catgaccttt tggtaaaagg acagttccat
     5341 cccctcaaga gtttggcgcc caataatatc tccgacattg ttaaccaaga tatccaatcc
     5401 gcccaggccc tccgccgcat cttgaactac attctttacg gtggcttcat ccgtcaaatc
     5461 gccgctaaca tccaccatct tggtgccgaa tgccttcact ttttcccgca gttcggcaag
     5521 atcctgtgct gtattttgag tttcacggtg ttgaaaccag tgaacagcga tacttgcccc
     5581 gcgtttggcg agttcgagcg agatggcccc gcccaggccg ttggctccgc cggtaatcag
     5641 caccttcttg ccctgtaatg atcccatgta tatcaatcct ttcacttagg gtcgaacgac
     5701 cccttttttc aaaagaatat tggcccggaa cgaagtatcc ccggtaataa ctactgcaaa
     5761 tgcgttcttg gcacgttcat aatactgata cctttcaatg tgttcgaatt cccggaagtc
     5821 cgattcggct tgacgaatga ttttgcggta ttcttcccag atcggcgggg ctgtttcttc
     5881 agcagttacg gacatgagta cggccgagta ttttacggtc ttatcgagcg gaaataaggc
     5941 gagcatgact tcgagtaatt gaggaagcac gagaccgtcg catcgaatca accgtttggc
     6001 acacgtggcc gccggaaaat tggcgtcggc cagcacgatt tcatcgctgt ggcccatctc
     6061 catcatggtc ttcattaatt caggggaaat gatcgctgga atatttttta acatcaaaag
     6121 cacctccttt tgttttgtca atttttgccc ttcgtgatat aatgaaaaca cacctgtatg
     6181 atacctatat attttatata acatctctgg aataaataca acccctggat caagtccaaa
     6241 attatgggta aatcgcccgg gtaaaacctt aaaatgataa gtcaaccggt aaatgcccca
     6301 tttaaatctt ttcagataaa tgttcaaacg gaaataagct caaaacaacg gtttcatttc
     6361 tgatttctcc ttgagcgcag cgaaaggaat ggtgaaaagt ggaaaaggac aggattcaga
     6421 tcgacgggaa ggaataccct ttataccggt gtcatacctt aatcatcgga tccggggccg
     6481 ccgcattaaa ttgcgcttgc catttatccg acctcggagt caatgacctg ttgattgtca
     6541 ccgagcaact gggaggggga acttccaaca attccggttc cgataagcag acttattata
     6601 aaatgtcgct ttttggcgaa gaccaggatt ccgtctatga aatggcccgg gccttattta
     6661 gcggcggctc catgcacggc gacatcgcct tggtggaagc gtccctctct gcccgggaat
     6721 tttttcattt ggtggagatc ggagttccat tccctcataa tcaatttggc ggatatgtcg
     6781 gctacaaaac ggatcacgat cccaaacagc gggcttcttc ggccggacct tggacttcca
     6841 atcagatgta ccagaaattg ttgatccagg cgcaacagaa gcagctacct atcatggatg
     6901 gttatgaggt tatctcgtta ctcaccgctc cggatgataa ggggcctcgg gccatcggcg
     6961 ccatagcgct ggataaagcc aaactccatt cgggaacagg ttcccttctg gttttacagg
     7021 cggaaaatat cgtaatggga accggcggac cgggggggct ttatcagacc tcggttttcc
     7081 ccaaggggca tttgggaagc accggaattg ccttggaggc gggcgcgacg gccgtcaacc
     7141 tgaccgaatg gcaatatggt atatcctcta cccaatttcg ctggagtgtc tccggtacat
     7201 accagcaggt aatccccaga tatataagta ccaatcagga tggcggcgac ccgcgggaat
     7261 ttttaaacga ctacttcccc acgccgggaa agctgggtac cgccaccttc ctcaaaggat
     7321 atcaatggcc gtttgatccc cgcaaggtca aaaactgggg ctcttcgctc attgatatcc
     7381 tggtatatca ggagaatgtt cttaagaaac gccgggtatt catggatttt cggaacaatc
     7441 cctcttttga ccaaaagtcc ggcgagtttt gttttgatca attggagccg gaagcctttc
     7501 agtatctgaa gaagtcgaac gggctctttg gtactcccat cgaacggttg gccaaaatca
     7561 atcccctggc catcgaactc tatgcccagc atggaatcga ccttcatcac gaacccctgg
     7621 agatcgcggt ctgcgctcag cataataacg gcggtttaaa gggtgatata tggtggcagt
     7681 caaacctgcg ccatcttttc ccggttgggg aagttaacgg aacccacgga gtatatcgcc
     7741 ccggcggttc ggcactcaac tccgggcagg ttggcgggtg cagagcggcc gagtatattg
     7801 cggcccgtta ttccggctat acgatggccg gtgaacaatt tgtcatgctt tctcaaaacc
     7861 aggttaccga caaacttcac ttaatgaatc aggtccttgg aaataacgaa aaatccgggc
     7921 aagatctggc gtccttccgc aaggagtttc aagaaagaat gagccatgtc gccgcccata
     7981 tccgtgaact gacagccgtt cgtgcggcag tgaaagaggc cgaagctcaa tggcagcgga
     8041 taaactccgg tgaagtgcga ttacaagacc attccgaact gactgcgttt ttccaaaacc
     8101 gccagctttg cctcacccag cttgcggttc tgaaaagtat cgaggcttat ttggaaaaag
     8161 gcggcggcag ccgcggttcc tatctggtac tggatcgaaa cggggaacgc atctccgatc
     8221 agatggatac ctcctggaat ttccggctgg agaacgaaga actccgtcgc tatacgttgg
     8281 aatgcaatta ttgttccaac gaatttagtt tgagctgggt gccggtacgg ccgattccaa
     8341 cggatgattt ctggtttgaa aacgtttggc gtgcttatcg tgataaagaa atctattgat
     8401 taccaacaca aaacgcgtga atgaatttac acaaaggaga ctgacaaatg attcgtaatt
     8461 ggaatgaaga atcgcttgac catcgtaatg ctttgcttta tgcaatggga gtatcgcccg
     8521 aggatgcaaa gcgccctgtc attggtatca ttaattcgtg gaacgagatg aacccgggac
     8581 attttgattt taaagatgtc atccaggagc tcaaggatga aatctacaat gccggcggat
     8641 tggcccgtga actgccggtg tcgggcattt gcgacggtat atgctccaat acgccgggag
     8701 atcgatatac cttaccggcc cgcgatcttg tcgcatccga ggtggaaaca accgcagagc
     8761 tgaatatgct tgaaggcatg attttattgg gtagctgcga taaggtagtc ccgggcatgc
     8821 tgatgggtat tatgcgggtg aacattccgg cggtcatgtt taccggcggt tatatggagc
     8881 cggggttcca taacgggaaa atgataacct tgacccatac gaagcaggcc tatgccgcgt
     8941 atattgcagg cgatattagc cagaaggagt ataaggatat tgtaagcgag gcgtgtccga
     9001 cgcccggtat ttgtccgttt atggggactg ccaataccat gtgcgcggca gctgaaattc
     9061 tgggattctc ccccgccggc aatgcaagtg taaaggctca aagtcccgaa tggcatgaaa
     9121 tggcgcggca ggcggcacgt aaaattgtcg aactcgtaaa aaaggagatt cgcccgctgg
     9181 atgttgtcaa cattgataat ttcgaaaatc tggtacgtta tatgatggct acaggcggct
     9241 ccaccaactc catcttgcat attccggcta tcgcgcggca ggcgggcatg gacataacac
     9301 ccgaattctt cgacaaaatc agcagggatg ttccggtaat caacgccatt tatccaaacc
     9361 acaagacata caccatggtt gatttttccg aggcaggcgg tctgggcgcc gttctgaaag
     9421 aacttgccgc ggccaggaag atcaatactt ccgccagggg tatgttcgga accgtcggag
     9481 agcagattgc caaggcgcaa aacaagaata aacaggttat ccatgaggtt aaaaacccca
     9541 ttaatgcaca gggcggttta gccgtattaa aagggaatat tgccaccgag a
//
LOCUS       DASVMB010000433         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_643515,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000433 DASVMB010000000
VERSION     DASVMB010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643515"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VF531_12190"
     CDS             complement(<1..632)
                     /locus_tag="VF531_12190"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEX7714765.1"
                     /translation="MTLFDNQGNMKLWYLEPAGDWNEALPIGNGSLGGMVFGGIAAEH
                     IQLNEDTLWYGGPSDRNNPDALKYLPEIRRLLFAGKLKEAERLSLFAQAGTPEMSRHY
                     EPLGDLHIDFDHDAKAANYRRELDLERAIVTVSYTIGGINYKREIFTSAVHKVLVVRL
                     TADKPGSISFHAKLGRAIYLDENVAAGEDTVLMKGTCGGKGGMEFCTFVKA"
     gene            complement(658..1674)
                     /locus_tag="VF531_12195"
     CDS             complement(658..1674)
                     /locus_tag="VF531_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7714766.1"
                     /translation="MVFQNAELYNVQAMVATGRQEYRLSRVPEEVRLKLNQRAQVMAF
                     NGCGTEIRFNLLGDRAKILLKREKADGLVSPYGIVEVYQGCFPGRYEISPQCIGVEPT
                     EIIIPKFNTEVLQKIAAENKSAFDPKLIRVLLPYDWSYYLMNIEGEIVPPQLGQTPGT
                     KYLAYGSSITHGGGAVSPSGGYAARLAQMLKTDLINLGFAGAAFLEEELADYIAARND
                     WDIATLELGINVIEDWDVEFFAQKADYFISKVAKSSPDKWIFCTDIFTCGRDFTNHPK
                     IESYRNTVRNIVEKLNMPKLVYICGRDLLKTGALLSSDGTHPSDRGMQEIAENFYEAM
                     KTKV"
     gene            complement(2093..2761)
                     /locus_tag="VF531_12200"
     CDS             complement(2093..2761)
                     /locus_tag="VF531_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX7714767.1"
                     /translation="MTEKLDYKKKYKDLYLPKQKPALIDVPAMNFIMVDGAGDPNDPN
                     GQYSEAMEILYGLSFTIKMSKMNGREPAGYFEYVVPPLEGLWWFENDEFDGTQLQDKS
                     KFNWISMIRQPEFVTADVFAWARAELQKKKPRLDLGKAHLESLAEGLCVQIMHLGPYA
                     NEAASIAPMEQFVLENGYVNAISDRLPSGQIRRHHEIYLCDPRKTEPDKLKTVIRHPV
                     KKSK"
     gene            complement(2764..>3213)
                     /locus_tag="VF531_12205"
     CDS             complement(2764..>3213)
                     /locus_tag="VF531_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX7714768.1"
                     /translation="YKIEEKEFGPISVLAIRFKGRYSDVGKYFGTIYKEAKGKASGES
                     FSCYYDDEYKEEADIEICVPTNGKVSGIRSVAKQVPGIKAICTTHIGSYDTLNYAYKA
                     LFDYAKEHNMECGLPSREIYHKGPGMIFKGNPNKYVTEIVIPVAKEG"
BASE COUNT          783 a          798 c          684 g          948 t
ORIGIN      
        1 gcctttacga atgtgcagaa ttccattccg cccttgccgc cgcaggttcc tttcatcaat
       61 accgtgtcct caccggcggc aacattctca tccaaataga ttgccctgcc cagtttggca
      121 tgaaaggaga tactgccggg tttatccgca gtcaaacgta ccaccagcac cttatgtacc
      181 gcgctagtga atatctcccg cttatagttg atgccaccaa tcgtatagct aactgtgaca
      241 attgctcttt ccaggtccag ttcgcgacgg taattagcgg ccttggcgtc atggtcgaaa
      301 tcgatatgta gatcgcccaa gggttcataa tgccgcgaca tttccggagt accggcttgg
      361 gcaaatagtg ataatctttc cgcttccttt agctttcccg cgaataaaag ccttctgatt
      421 tcgggtaaat atttcagcgc atccggatta tttcgatcac tgggacctcc ataccaaagg
      481 gtgtcttcgt tgagttggat atgctcagca gcaattccgc cgaaaaccat cccgccgaga
      541 ctgccgttac cgatggggag cgcttcattc cagtccccgg caggttccaa ataccaaagc
      601 ttcatgttac cttgattgtc aaaaagggtc acagaatcac ctcatggatt cataaactta
      661 tactttcgtt ttcattgctt cataaaaatt ttccgcaatt tcctgcatcc ctcgatccga
      721 aggatgggta ccgtctgagg agagtaaggc gccggtcttt agcaggtctc taccgcatat
      781 atagacaagt ttgggcatgt tcagcttttc gacaatattc cggaccgtat tgcgatagct
      841 ttcgatcttc ggatgattgg taaaatcccg gccgcaggta aatatgtcgg tacaaaagat
      901 ccatttatcg ggactgctct tcgccacctt ggatataaaa tagtccgctt tctgcgcaaa
      961 gaattctaca tcccaatctt caattacatt gatacccagt tctagcgtgg ctatatccca
     1021 atcgtttctg gctgcgatat aatcagccaa ctcttcttct aaaaaggctg cgccggcaaa
     1081 gcctaaattg atcagatcgg ttttcagcat ttgggcgagc cgggccgcat aaccgccgga
     1141 cggacttacg gctccgccac catgagtaat cgaggaaccg taagcgagat attttgtacc
     1201 cggtgtttgc cccagttgcg gcggaacgat ttcgccttca atattcatta aataataaga
     1261 ccagtcgtaa gggagaagga cccggatcag cttgggatca aaggctgact tattttctgc
     1321 agcgattttc tgtaacactt cggtgttgaa tttcgggatg ataatctctg tgggttcgac
     1381 tccaatacac tggggcgata tctcatatct ccccggaaag caaccttggt aaacttctac
     1441 aatgccataa ggagacacga gcccgtcggc tttttccctt ttcaacagga ttttagcccg
     1501 atccccgagt agattaaagc gaatttccgt gccgcaaccg ttaaaggcca taacctgagc
     1561 tctttgattc agtttgaggc ggacttcctc cggtactctg cttaatctgt actcctgccg
     1621 gccggtggca accattgcct gaacattata taactccgcg ttttggaaaa ccatttgatt
     1681 taccttctta attttacgtg tatccagcat ctaccccggt tgtttcaacg gagatgatgc
     1741 aaataccttc tttaaaacat ttaaaaatta tactatattg ttagaatggt tgatcgaata
     1801 ccactatttg taattatctt cggtttcgct aataatcgtg aaaaaataaa tctttatacg
     1861 gagaattttc ctcttttcgg atctaaagaa catcgtctaa aaaataaatt tcgtgtattt
     1921 ggtgattttc gtggttaaaa agtttctatc tgtatgccta ttttattcac aacgaaacac
     1981 acaaaagaca tgaaaatggt tatggcttta tttacggcca ttgaagaaaa aatttaccca
     2041 gagggcggta tccggctctc cgggtaagct aaatctgctc gtttaccttt tgttattttg
     2101 acttcttcac cggatgcctg attacagttt tcagtttgtc cggctccgtc ttgcgagggt
     2161 cgcataaata gatttcatgg tgccgccgga tttggccgga aggcaagcga tcggatatgg
     2221 catttacgta tccattctcc aaaacgaatt gttccattgg tgcgatggag gcggcttcgt
     2281 ttgcataggg gccaagatgc attatttgca cgcacaagcc ttccgccaga gattccaggt
     2341 gagccttgcc cagatcgagc cggggctttt tcttttggag ttccgcgcgg gcccaggcaa
     2401 aaacatctgc tgtgacaaat tccggttggc ggatcatgga gatccagtta aatttcgatt
     2461 tatcctggag ttgcgtgccg tcaaattcat cgttctcaaa ccaccatagc ccttccagag
     2521 gcgggacgac atattcgaaa tatcccgccg gttcccgccc gttcatcttg ctcatcttaa
     2581 ttgtgaagga cagtccataa aggatctcca tggcttccga gtattggcca ttcggatcgt
     2641 tggggtcccc ggcgccgtcg accatgataa aattcatggc cggaacgtca attagcgcgg
     2701 gtttttgctt cggcaaatac aagtccttat attttttttt ataatccagt ttctcagtca
     2761 ttttcatcct tccttcgcaa ccggtattac gatctcggta acatatttat tgggattgcc
     2821 tttgaagatc attccgggcc ccttatgata gatctcccgg gaaggcaacc cgcattccat
     2881 attatgttcc ttggcataat caaataatgc cttgtatgcg tagttcaacg tatcgtagct
     2941 gccgatatgt gtggtgcaga tagccttgat tcccggcacc tgttttgcaa cgctgcggat
     3001 tccagacacc ttgccattgg taggcacgca tatttcgatg tccgcctctt ctttatactc
     3061 gtcatcataa taacagctaa acgattcccc ggaagcttta cctttcgctt ccttatagat
     3121 tgtcccgaag tactttccga cgtcgctgta cctaccctta aaccgtatcg cgagtaccga
     3181 tatcggccca aactctttct cttcaatttt ata
//
LOCUS       DASVMB010000434         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_52672,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000434 DASVMB010000000
VERSION     DASVMB010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52672"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VF531_12210"
     CDS             complement(<1..452)
                     /locus_tag="VF531_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEX7714769.1"
                     /translation="MPNNQYFLLLIVVIVVVLFVTFASVLKRYKRCPSDKILVVYGRT
                     GKSKDGTARSATCIHGGAAFIWPVIQDYQFLDLTPFSIEVNLTNALSKQNIRVDVPSR
                     FTVGISTEQGVMTNAAERLLGLKLEVIQDLAKDIIFGQLRLVIATMDI"
     gene            complement(485..1072)
                     /locus_tag="VF531_12215"
     CDS             complement(485..1072)
                     /locus_tag="VF531_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714770.1"
                     /translation="MMVPWWDTIPAFEKIFWYFAIPFSLVFSIQIILTFIGIDWHDSG
                     LDAHGGIDIQHDTDFLAGFHLFTLRNFIIFFTGFGWAGIFAVHAGFGQIASVFFAFII
                     GLILMVAVAGMFFLMTKLTQSGNIKMTNAVNSSGLVYLPIPAKRSGLGQVQLTVQGSV
                     REVEAMTDGETLPTGTPVTVTEVVDEKILVVKKSL"
     gene            1501..>1945
                     /locus_tag="VF531_12220"
     CDS             1501..>1945
                     /locus_tag="VF531_12220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7714771.1"
                     /translation="MIRTMMRDFTINNGDIDGAIDIMREVALWCEATGKNMWRMEQLS
                     KEVLLNGLTAENFYIGKIGSKNAAAMILQWYDPLFWPELHEGESGFVHKLCVRRKYSG
                     QGLSQRMIARAIEECKARGIHYLRLDTGWHRPRLCELYESLGFVKV"
BASE COUNT          570 a          406 c          472 g          497 t
ORIGIN      
        1 tcgatatcca tcgtcgcaat gaccagccgt agctgaccga agataatatc tttggccaga
       61 tcctgaatga cttccaattt aaggccaagc agtctttcgg cggcgttggt catgactccc
      121 tgttcggtgg agatacccac cgtaaaacgg gaagggacgt cgacccggat gttttgttta
      181 ctcaacgcgt tggtcaagtt gacttcaatg gagaagggcg tcaggtcgag aaattgataa
      241 tcttgaatca ccggccagat aaaagcggcg ccgccatgaa tacaagtcgc ggagcgggcg
      301 gttccatcct ttgattttcc ggtccggccg tagaccacca ggattttgtc cgaggggcac
      361 cgtttgtaac gtttcaggac agaggcaaaa gttacgaaaa gtacaacgac aattacaacg
      421 attaacaata aaaaatactg attgttcggc atcggctcag tctcctatat ttcgagtttc
      481 gtaatcacaa actcttttta accaccaata tcttttcatc aacaacctcc gttacagtaa
      541 ccggggtacc ggtgggtaac gtctccccat cggtcatggc ttccacctcg cggactgagc
      601 cttgaacagt aagttgcacc tgtcccagac cggatctttt ggcgggaatc ggaaggtaga
      661 caagaccgga agagtttacg gcattcgtca ttttgatatt gccgctttga gtaagcttgg
      721 tcatgaggaa aaacatcccg gccactgcaa ccatcaaaat cagtccgatg ataaaggcga
      781 aaaaaacaga agcaatttgg ccgaaccctg catgcaccgc aaaaatccca gcccaaccga
      841 atccggtaaa gaagatgata aaattcctca aagtgaaaag gtggaaaccg gccaagaaat
      901 cggtatcatg ctggatatca atgccgccgt gggcatccag gccactgtcg tgccagtcga
      961 ttccgataaa agtcaagatg atttggatcg aaaaaaccag cgagaatgga atcgcaaaat
     1021 accagaagat tttttcaaaa gcggggatgg tgtcccacca tggaaccatc atcagttcct
     1081 ccttcttcgc gtactctagt aatcgttaag caagggataa aacctcagca aaaagcatca
     1141 ttaacctttc gcgattcatt ttatcatctt cgtagcagca gaacaagtta agcttttaca
     1201 aaaaaatcaa ggttgatata atatattaaa tttcaaggat tttcaccggt ttgatataat
     1261 ataatggttc atacataaat aacctcgttt atatatgatt ccgggcagaa gcaatctgcc
     1321 gtagagtggt tctttcagtg cattttcaac ggtaatgaat ccgggtttta tggttctagc
     1381 atttttgcat ttaaagagga ttcgattgtt agtctaaatg agtatgaaat ggaaaaggac
     1441 ggccgagaat catttgtttt tggaagtttt aataatatgt tacataccga cggaggcacg
     1501 gtgatacgaa cgatgatgcg ggattttacc ataaataatg gtgatatcga tggggccatt
     1561 gatatcatgc gggaagtcgc actgtggtgt gaggctacgg gaaaaaacat gtggcgcatg
     1621 gaacaattat ccaaagaagt tttactaaac ggacttacag cggagaattt ctatatcgga
     1681 aaaatcggat ccaagaatgc tgccgctatg atcctgcaat ggtatgaccc tttgttttgg
     1741 ccggagttgc atgagggcga atcgggtttt gttcataaac tatgcgtccg gagaaagtat
     1801 tcaggccaag ggttgtcgca acgaatgata gcccgggcga ttgaggaatg taaggccagg
     1861 gggatccatt atttgcgact tgacacgggg tggcatcggc caagactctg cgagctatat
     1921 gaaagtttgg gatttgtgaa ggtgg
//
LOCUS       DASVMB010000435         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_199964,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000435 DASVMB010000000
VERSION     DASVMB010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199964"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="gltA"
                     /locus_tag="VF531_12225"
     CDS             complement(<1..1202)
                     /gene="gltA"
                     /locus_tag="VF531_12225"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX7714772.1"
                     /translation="MPRQVMPKQNPELRKHNFDEVALGLSEVQAREEAARCLSCKNPL
                     CVQGCPVEVDIPGFIGRLKENDPTGAVAKIKEKNSLPAICGRVCPQESQCESRCVLGK
                     KGDPVAIGALERFAADQTAAGVTKPVPINKNGFKAAVIGAGPSGLTAAADLALQGFDV
                     TIFESLHAAGGVLQYGIPQFRLPKEIVAREVDYIKQLGVKVKTSTLVGQTVTVAELFG
                     SGFDTIFIGTGAGLPYFLNIPGENLNGVYSANEFLTRVNLMKAYRFPEFDTPVRIGER
                     VAVVGAGNVAMDAARTSLRLGAKEAYIVYRRSAEEMPARRDEIENAEEEGVIFKLLTN
                     PTRIIGNERGEVSALECIRMELGEPDASGRRRPVPVSGSEFQFSVDNVIAAIGQGPNP
                     VLLRKTEGL"
     gene            complement(1187..2038)
                     /locus_tag="VF531_12230"
     CDS             complement(1187..2038)
                     /locus_tag="VF531_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX7714773.1"
                     /translation="MEVLSREILAPRIARLVVKAPAIAAKAEPGHFVVVRLNERAERI
                     PLTIADFDRDKGTITLIVQEVGKSTALINRMNPGDFFADVLGPLGTPYPISRLGTVVG
                     VAGGLGAAPLYPKVKALHEAGNRVIVILGAQRQELLILMDELKAISDQLLVATDDGSY
                     GQKGLVTEPLRQVLDMEPVDEVIAIGPVPMMDACCKLTRERNVKTVVSLNAVMVDGTG
                     MCGGCRVTVGGQTKFCCVDGPSFDGLAVDFAELRQRQRYYNEQEKQAYQRLLDSDGME
                     GCRCRAK"
     gene            complement(2280..2717)
                     /locus_tag="VF531_12235"
     CDS             complement(2280..2717)
                     /locus_tag="VF531_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714774.1"
                     /translation="MMSPEMAGDLLIYSLPLLTTGATVALTATGLYLGSKLHNKRLAQ
                     AVMLLDQIVIDVVKELNQTVVADLKKARADGKLTPDEAAQIKSKAIELIVSRLGLDLI
                     RVIERSLGSVLLIIATKIEATVFDCKRSAKHQPSKSLKLAVAR"
     gene            complement(2882..3316)
                     /locus_tag="VF531_12240"
     CDS             complement(2882..3316)
                     /locus_tag="VF531_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714775.1"
                     /translation="MKLLDKNGWLNLVLGMFGFYSVYTLMELLVGRYGPRVVAATTAA
                     AHISENYFWLISFIFGLCAALVLVVIFCKPRSPLAVGIGVLVGGILGMLPTLYRAIFN
                     LDRFFRKEGLAAIHYLLPHLVVLAGALIALYVAWLSNKMAKS"
     gene            3532..3607
                     /locus_tag="VF531_12245"
     tRNA            3532..3607
                     /locus_tag="VF531_12245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3565..3567,aa:Arg,seq:cct)
BASE COUNT          914 a         1043 c          826 g          898 t
ORIGIN      
        1 agtccttcgg tcttgcgaag taaaaccggg ttcggcccct gaccgatggc tgcgatgacg
       61 ttatccacag agaattgaaa ctccgaaccg gaaacgggca ccggccgtct tctgccgctg
      121 gcgtcaggct cgcccaattc catccggatg cattcgaggg cgcttacttc accgcgttca
      181 tttccaatga tccgagtggg attggtcaac agcttgaata tgaccccttc ctcctcggcg
      241 ttctcgatct catcgcgccg ggccggcatc tcttccgccg aacgcctgta gacgatataa
      301 gcttccttgg ctccaagccg gagtgaggtc cgggctgcat ccatggccac gtttcctgcg
      361 cctactacag ccacgcgctc tccaatccgg accggggtat cgaactccgg aaaacggtaa
      421 gccttcatca gattgactct ggttaaaaac tcgttggccg aatatacccc gttcaaattc
      481 tcgcccggaa tattcaagaa atatggcaaa ccggctccgg tgccgatgaa gatggtgtcg
      541 aaaccggaac caaaaagttc ggccacggtt acggtctgac ctaccaaggt actggtcttg
      601 acctttaccc ccaattgctt gatataatcc acttcacggg ctacgatctc cttgggcagc
      661 cggaattgag gaattccgta ctgcaagaca ccacccgcgg catggagcga ttcaaaaatg
      721 gtaacatcga acccctgcaa agccagatct gccgcagcgg taaggccgga tgggccggcg
      781 ccgattaccg cagccttaaa accattttta tttattggga ctggtttggt aacgccggcc
      841 gcggtttggt cggccgcaaa cctttccagc gcaccgatgg ctaccggatc acctttcttc
      901 cctagaacgc aacggctctc acattgggat tcctgggggc aaacccggcc gcagatcgcc
      961 gggagactgt tcttttcctt aatcttcgcc actgccccgg tagggtcgtt ctctttaagc
     1021 cttccgataa aaccgggaat atcgacttcg accgggcatc cctggacaca aaggggattt
     1081 ttgcagctca agcaacgggc tgcttcctct ctggcctgga cttcggagag tccgagcgcc
     1141 acttcatcga aattatgctt ccgtaattcc ggattctgtt tcggcattac ttggcgcggc
     1201 atctgcaacc ctccattccg tccgaatcca atagtctctg gtaagcctgt ttttcctgct
     1261 cgttataata gcgctgcctc tgccggagtt cggcaaagtc aaccgccaga ccgtcaaagg
     1321 agggtccatc cacacaacag aatttcgtct ggccacccac tgtcacccgg caaccgccgc
     1381 acattccggt tccatcgacc ataaccgcat ttaaactgac tacggttttg acattccgtt
     1441 cccgggtaag cttgcaacag gcgtccatca tcggaaccgg cccgatggcg atgacctcgt
     1501 ccaccggttc catatctaaa acctggcgca acggttcggt taccaaaccc ttctgaccgt
     1561 aactgccgtc atccgtcgcc accagcaact gatcggagat ggcttttaac tcatccatca
     1621 aaatcaataa ctcctgccgt tgcgcgccca gaatcacgat gacgcggttc ccggcctcat
     1681 gcaacgcctt caccttgggg taaagcggcg cggcccccag acccccggcg actccgacca
     1741 cagtccccag cctactgatg gggtatggag ttcccaatgg ccccaaaaca tcggcaaaga
     1801 agtcccccgg attcatccgg ttgatcaagg ccgtactctt acccacttcc tggacgatca
     1861 gtgttatcgt acccttgtcc cgatcaaaat cggcaatggt taacgggatc cgttcggcgc
     1921 gctcgttcag cctaaccacc acaaaatgcc ccggttccgc cttggcagcg atggcgggtg
     1981 ccttcacaac caacctggcg atccggggcg ccaatatctc tcttgataaa acctccaaat
     2041 cttcgccccc cgtgcaacat catgtcggtt ttaacaaaac cgttttgtcg cggcttcttt
     2101 tgccgcaacg attctcctga agcgatctta ataatgtaac ggtatttcat tttatgccga
     2161 tcgcagatct gctatgaaac ttgaaaatga tttatataag tataacaaaa ttcgttgcgc
     2221 cgggcaagtc tatgtggaat acattttcac tatatgaaaa tcaagtttat tttctgccat
     2281 cacctggcta cagccaattt caaagatttt gaaggttgat gtttggctga tcttttacaa
     2341 tcaaacacgg ttgcttcgat tttggtcgcg atgatgagta ataccgaacc caacgaccgt
     2401 tcaatgacac ggatcagatc caatcccagc cgggaaacga ttaactcaat ggccttactc
     2461 ttaatttggg cggcctcatc cggtgtcagc ttgccatcag ccctcgcctt cttaagatca
     2521 gccactacgg tttgattcaa ttccttaaca acatcgatga cgatttgatc cagtaacatc
     2581 actgcctgtg ccaaccgttt attatgcaat ttgcttccca gatataaccc ggttgctgtt
     2641 aatgccacgg tagccccggt agtaagcagc ggtagcgaat aaattaacaa atcccccgcc
     2701 atttcaggac tcatcatttt ccctcctccg atatgaatag tattcaaaaa tatcatatgc
     2761 cgcagtacga cgaccgggtg ccaaccgccc catctcttaa tccggcataa gaaggaattg
     2821 agcaaaatgg aaacaaaaaa gcccggaaca tgttccgggc caacttgggc agtttatgta
     2881 ttcatgattt ggccattttg ttactgagcc aggcaacata gagggcgatt aaagcgcctg
     2941 ccagcaccac caaatggggt aggaggtaat ggatggcagc taacccttct ttgcggaaaa
     3001 agcgatccaa attgaaaatc gctcggtaga gtgtaggaag catccctaaa ataccgccta
     3061 ctagaacccc gatgcctacg gccaacgggg aacgaggttt acagaagatg actactaata
     3121 ccaaagcggc acatagacca aagataaaag agatcagcca gaaataattc tctgaaatat
     3181 gggctgccgc tgtggtggcg gcaaccaccc ggggcccgta ccgacctact aacagttcca
     3241 taagggtata aacagaataa aacccgaaca tgccgagaac cagattaagc caaccattct
     3301 tatccaacag tttcatcttc ttcctccttt tgggttgtgt aggaaccatt aatccgagtt
     3361 gacaaatatt atttatgata taaaatttga catccattgg cagattcctg ccaaccggca
     3421 ctcatcgtca aattggctcc gtcactgtga tcccgattcg attttccctg tcaaaacctg
     3481 aaagttgtaa aaccacttag gatttgctat aatattaatg cttaatttaa cggccccgta
     3541 gctcagcgga tagagcagcg gtttcctaaa ccgtgtgtcg gatgttcaat tcatctcggg
     3601 gccgccatag aaatgacaag ggtttgccgg agcggcaggc tctttatttt ttattcccat
     3661 ccaagcgagg atttttttat t
//
LOCUS       DASVMB010000436         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_643829,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000436 DASVMB010000000
VERSION     DASVMB010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643829"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /gene="livG"
                     /locus_tag="VF531_12250"
     CDS             complement(<1..128)
                     /gene="livG"
                     /locus_tag="VF531_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948352.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HEX7714776.1"
                     /translation="MSSNVLEVKDLSIFFGGLKAVSGFNLNLADGELVGLIGPNGAG"
     gene            complement(125..1144)
                     /locus_tag="VF531_12255"
     CDS             complement(125..1144)
                     /locus_tag="VF531_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7714777.1"
                     /translation="MKDNHMKGTASNPKIKNILILAGISLLILAVTSVLIYRGDIDEY
                     TAQILTLGGINVIISLSLNLISGFTGQLALGQAGFMAIGAYSTAIFVMQLHLPMAPAI
                     VIGGLVTALFGLLIGFPTFRLKGDYLAIVTLGFGEIIRVIMINMESLTGGAAGLKGIP
                     PFSEDYTWGPMVSFIWVYVFIVLTIFLVSNLIKSSPGRAIISVREDEIAANAMGVNVF
                     YYKMFSFTVSAFIAGIGGGLYALFFGYLNPTMFNFMKSMDFVVIVVLGGMGSITGTVI
                     TGFALTYLQEFLRILQDYRLVIYPLVLILIMLFKPSGLMGTKEFSVGAIFQKKKNTLK
                     GGEAQ"
     gene            complement(1149..2048)
                     /locus_tag="VF531_12260"
     CDS             complement(1149..2048)
                     /locus_tag="VF531_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7714778.1"
                     /translation="MGFTQEFLQQLVNGLALGSIYALIALGYTMVYGIIKLINFAHCD
                     VMMLGAFAGFFVLSAYGVTPVTFLLSFIVAMLICAIIGVVMERTCYKPLRNAPRINAL
                     ITAIGLSFFIENGARVLPVIGPNPRQFPTFSNVNYNIIGVNISQVQILIFLVSIGLML
                     LLNYIVLYTKIGKAMRAVSFDRNAAFLMGINVNRVISFTFAIGSALAAAAGILFSSAY
                     PQVEPYMGVMPGLKAFVAAVLGGIGSIPGAMVGGIIMGVAETLTKGFISSELADAIAF
                     GILIIILVVKPSGIFGEKESEKV"
     gene            complement(2350..3504)
                     /locus_tag="VF531_12265"
     CDS             complement(2350..3504)
                     /locus_tag="VF531_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7714779.1"
                     /translation="MGKKRVLTLVALAAMLIAMIAFTVTVNGAAQTIKIGTILPISGA
                     ISAYGIESRDAMNMAVAEINAKGGVLGKKLELVVEDDENSPDKTMNAIKKLVSRDRVT
                     AFVGAMTSKCTLAITKYAQAKKLVMVTPTSTNDTVTEAGDYIFRICYTDSFQGQVVAQ
                     YAFETLKTKKAAILYDNTNDYSKGLTNNFKEKFEKLGGKIIAQESYSSTDKDFNAQLT
                     KIKAAGPEVLFIPDYYSTVSLIAKQVRAQGLNIPMVGADGWDEITNNAGDEVINSYYS
                     NHYSPEADDPQVKNFVKNFQAKYKGVTPNALAALSYDATYVMADAIKKAGSTDSAKIR
                     AALLKTNGKFVTGSIRFDAKRNPIKSAVMLKIVKGSDGKLTTQYAGTVNP"
     gene            complement(3855..>4646)
                     /locus_tag="VF531_12270"
     CDS             complement(3855..>4646)
                     /locus_tag="VF531_12270"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7714780.1"
                     /translation="MRVRGFTQDDAHLICSPDQMPQEIDRVLNFCLKMIRSFGFQDFK
                     LYLATKPEKSVGDSAQWQAATEALQAAIAKTGIPSEVDEGGGAFYGPKIDLKIKDALG
                     REWQCSTIQFDFNEAERFGLTYKGSDGQDHQPYMIHRALLGSLERFFGVLIEHYEGAF
                     PIWLAPAQAIVLPVLPEQNDYARSVMETLRQNGIRAEIDNRNEKIGYRIREGQLQKIP
                     YMLVVGEREQQEQTVSVRARKEGDLGPQPLTRIQTTILQQIKEHC"
BASE COUNT         1271 a         1200 c         1013 g         1162 t
ORIGIN      
        1 ccagcgccat tgggtccgat caaccccacc aattcgccat cggccaaatt caagttgaaa
       61 ccggagaccg cctttaagcc tccgaaaaag atggagagat ccttcacttc gagaacgttg
      121 gaactcactg ggcctcacca cctttcaggg tgttcttttt cttttgaaat atggccccca
      181 cggaaaactc tttcgtgccc atcaaaccgg aaggcttgaa taacatgatc aggattaata
      241 ccagaggata gatgaccaac cggtaatcct gcagaatcct taagaactcc tgcaaatagg
      301 tcaaggcaaa accggtgatg actgttccgg ttatactgcc cataccaccc aaaactacaa
      361 tgaccacaaa gtccatggat ttcatgaaat tgaacatggt gggattgaga tacccgaaga
      421 agagagcgta taaaccgccg ccgattccgg caatgaacgc cgataccgta aaagaaaaca
      481 ttttatagta aaagacattt actcccattg cattggcggc aatttcatcc tcacgaaccg
      541 agatgatagc ccttccgggt gaggatttaa tcagattgct cactaggaag atggtaagca
      601 ctataaaaac ataaacccaa ataaacgaga ccatcggccc ccaagtataa tcttcgctaa
      661 aaggcggtat tcctttcaat cccgctgcac cgccggtgag actctccata ttgatcataa
      721 ttacccggat gatctcaccg aatcccaaag ttacgatcgc caggtagtcg cccttcaacc
      781 ggaaggtggg gaaacctatt aacaatccga ataacgcggt aaccaatccg ccgataacaa
      841 tcgccggcgc catcggtaga tgcagttgca tcacgaaaat cgccgtagaa taagctccga
      901 tagccataaa tcccgcttgc cccaatgcca gttggccggt gaaaccggag atcagattga
      961 ggcttaaaga gatgatgacg ttaattccac ccagagtaag aatctgggcg gtatattcgt
     1021 cgatatcgcc acgataaatt aaaacactcg taacggccag gatcagaagg gatatacccg
     1081 ctaaaatgag aatattcttg attttgggat tggatgccgt ccctttcata tgattatctt
     1141 tcataagttc acaccttctc gctttctttt tcgccaaaga ttccggaagg cttcaccacc
     1201 aaaataatga tcaggatacc gaacgcgatg gcatcagcaa gttccgaaga gataaatcct
     1261 ttggttaagg tctcggcgac acccataata atcccgccca ccatggcgcc ggggatacta
     1321 ccgatcccac caagtaccgc ggcgacaaat gccttaagcc ccggcattac tcccatgtag
     1381 ggttcaacct gaggataggc gctggagaat aaaattccgg ccgccgctgc aagcgccgaa
     1441 ccgatagcaa aggtgaatga gatcacccgg ttaacattga ttcccattaa aaaagcggcg
     1501 tttctgtcaa aggagacggc ccgcatcgcc ttaccaatct tggtgtacaa aacaatataa
     1561 tttaataaca acatcaatcc gatggaaacc aaaaaaatca agatctgaac ctggctgata
     1621 ttcactccga taatgttgta attgacattg gaaaaggtgg gaaattgcct ggggttgggc
     1681 ccaatgaccg gaagaacccg cgccccgttt tcaataaaaa atgaaagacc gatggcggta
     1741 atcaaggcgt tgattcgggg ggcattccgt aacggcttgt aacaagtccg ctccatcacc
     1801 actccgatga ttgcgcaaat caacatggct acaataaaag aaagcaaaaa cgtgaccgga
     1861 gtgacaccat aggcggaaag cacaaaaaat cccgcaaaag cgcctaacat catcacgtca
     1921 caatgggcga agttgataag cttgataatt ccgtaaacca tagtatatcc aagagcaatc
     1981 aaggcgtaga tgctgcctag ggccaaaccg ttcacaagtt gttgcaagaa ttcctgtgta
     2041 aaacccatgc tcccacctct ttaatctagt cttacaatgg agattgcttc accagaggtt
     2101 gcttctccga gaggttactt caccgctttt tgatcgggcc tacttcaatc cggacttcca
     2161 aaaagaccga gtggccgttc ttcacaagta tcggacggaa tccggcaaag gcttggctta
     2221 tcgtaaataa tgggagaata tgccattctc ccattatcca tccgtccata tttattatat
     2281 ctcaatttca aaaagatagg gatgatcaat tcgtatcaaa actaccaatt gtatcatcca
     2341 gtccccgaat cagggattga ccgtcccggc gtattgggtc gttagtttac catcgctgcc
     2401 ttttacaatc ttcagcatta ctgcggattt aatcgggttc cgcttcgcgt cgaaccggat
     2461 agaacccgtg acgaacttgc cgttagtctt taaaagtgca gcccgaatct tggccgaatc
     2521 ggtggaaccg gcctttttaa tcgcgtcagc cataacatag gtggcgtcat aactcagtgc
     2581 agcaagggca ttcggagtta cacccttata ctttgcttgg aaattcttaa cgaagttctt
     2641 tacttgtgga tcatccgctt ccggagaata gtgattacta taatagctat tgatcacttc
     2701 atcaccggcg ttattggtga tctcatccca accatccgcc ccgaccatcg gaatattcaa
     2761 gccttgggcc cggacctgct tggcaatgag cgaaactgtg ctgtaataat cgggaatgaa
     2821 caaaacttct ggaccagccg ctttaatctt ggttaactgg gcgttaaaat ccttatcagt
     2881 ggaactgtat gattcctggg cgatgatctt gcccccgagc ttttcgaatt tttccttgaa
     2941 gttattggtc aagcccttgg aataatcatt ggtgttgtca taaagaatgg cggccttttt
     3001 agtcttcaaa gtctcaaaag cgtattgggc aaccacttga ccctggaacg aatcggtata
     3061 gcaaatccgg aagatatagt caccggcttc ggtaaccgtg tcattggtac tggtgggggt
     3121 aaccatcacc agctttttgg cctgggcgta cttggtaatg gccaaggtgc atttactggt
     3181 catggcacca acaaatgcgg tcacccggtc ccgtgagacc aatttcttta tggcattcat
     3241 ggttttatcg ggactgttct cgtcgtcttc cacgaccaac tcaagcttct taccgaggac
     3301 tccacctttg gcattgatct ccgctaccgc catgttcatg gcatcccggc tttcgatgcc
     3361 gtatgcggaa attgcgccgc tgatcggcag aatggtaccg attttaatcg tttgcgccgc
     3421 gccattcacg gtaactgtga aagcaatcat cgcgatcaac atggctgcta aggccactaa
     3481 tgtcagaacc ctttttttgc ccattggaaa accccctcca aattttagtt tcttcacctg
     3541 tccacgaaag acctaggctc aagctttcct ttaaaactgc gtcccgtttc caggtataac
     3601 tttactaaga attatatatc agacatatta ttttcgcaat ggttaaatcg gggtttttgt
     3661 aaggtaactg tcaatattat atcagacata ttcgtaaaat cgcagtttct tcgaaataat
     3721 cccaaaatta tcctaaacat tcactcgtat atatccgcta cagtattcat aatgctgtat
     3781 acttcaagcg tatgtaaagt aaatgttaga tcttggggcc ggagtactta acccgccggc
     3841 cccaatgaaa gaacctaaca gtgctccttg atctgctgta agattgttgt ctggatccgg
     3901 gtcaatggtt gaggacccaa atcgccttct ttccgggccc gtacagagac agtctgctcc
     3961 tgttgctccc gttcgccgac taccagcata tagggaatct tttgcagttg gccctcgcga
     4021 atccggtaac cgatcttctc gttgcggtta tcgatttcag cccggatccc gttttgcctc
     4081 aaggtctcca tcaccgaccg ggcgtaatcg ttctgttccg gcagcaccgg gagaacgatg
     4141 gcctgagccg gcgccagcca aatagggaac gccccctcat aatgctcgat taaaaccccg
     4201 aagaaacgtt ccaaggaacc aagcagcgcc cgatggatca tataaggttg atgatcctgg
     4261 ccgtcactgc ctttataggt caaaccgaac cgttccgcct cattaaaatc gaactggatg
     4321 gtggaacact gccattccct gcccagtgcg tccttgatct tcagatcgat tttcgggcca
     4381 tagaatgcgc cgccgccctc atctacctca ctggggatac cggtcttcgc gatggcggcc
     4441 tgtaacgcct cggtggctgc ctgccactga gcgctgtccc ccaccgattt ttccggcttg
     4501 gtcgccaaat acaacttgaa gtcttgaaat ccgaaggaac ggatcatctt cagacaaaaa
     4561 ttcaaaaccc ggtcgatctc ttgcggcatc tgatcgggac tgcagatcaa atgggcatca
     4621 tcctgggtga aaccacgcac ccgcat
//
LOCUS       DASVMB010000437         5781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_568380,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000437 DASVMB010000000
VERSION     DASVMB010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568380"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF531_12275"
     CDS             <1..250
                     /locus_tag="VF531_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7714781.1"
                     /translation="ARTFDYRAGDVGYVPVGYVHYIQNTGNEIVWFLEAFKSDRFEGV
                     SLNQMMALTPPGLIASNLQIGPEFLNALRKVDCPVVPC"
     gene            519..1061
                     /locus_tag="VF531_12280"
     CDS             519..1061
                     /locus_tag="VF531_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714782.1"
                     /translation="MMKQLKNMQAWLVLLAFIITLSALFGGRLLSNRLQVTEPLRKEM
                     AAIKAVKKFKVDPENNGLKVTLNLGRVANLQDVLERVEEKVEHFYNKPVKTIVLVDHS
                     NQRLRQVRYQLAFNLEEARVSGRYLQLKSALESYQGVKAQVYFSKDYFFIQLEDGSYY
                     HYEAVPRGQQTVQPTGGDPA"
     gene            1058..2545
                     /locus_tag="VF531_12285"
     CDS             1058..2545
                     /locus_tag="VF531_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7714783.1"
                     /translation="MMRRFEWLIGIGLGVLVYLFWIGVDLTPLVLIGGALGILYLLTQ
                     GRLGRGFETVGNNKTVTRVDTGVRFDDIGGQEVAKRELLEALDFVRDQAKISALGIRP
                     LKGILLTGPPGTGKTLLAKAAANYTDSVFVSASGSEFVEMYAGVGAQRVRQLFNKARQ
                     LASKMAKHSAIVFIDEIEVIAGKRGNHTSHLEYDQTLNQLLVEMDGIKPNETVQIVVI
                     AATNRADLLDGAILRPGRFDRQVKVDLPDKEGRMHILNIHAKGKPLDSEIDLEEVARD
                     TFGFSGAHLESLINEAAILAYRRGKSCIQMDDIREAIDKVMLGEKLEHRPDPDELKRV
                     AIHEVGHAMVSEVLRPDSVATLTVIPRGKALGFMRQAQAQDHYLYTREFLLDQVAICL
                     GGAISEELIYGDWSTGASNDFEQATSLAKQIITTGLSRLGIIQEDLAGKDRIYEVMTE
                     ILEEQKSRVRELLKKRSPIIPAITETLLEQEKVSGVWFREQLKCA"
     gene            complement(2603..3982)
                     /locus_tag="VF531_12290"
     CDS             complement(2603..3982)
                     /locus_tag="VF531_12290"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7714784.1"
                     /translation="MKKFWISLGIIILLFAWHIVPARAVENDRIGILDFSGADDPEYC
                     IRAANRLTSILSHFGKYEIVERAEMDRILKEQEFQESGAVDQETALEAGRIAGITTAY
                     LGNIDQLSATYDKDLKEYTATAKVTIKIISVDTAEILSVIQASAVSTNGGEKESLHAA
                     LDKCFGYRFQAQLREFLAPYSSIQSISGDTIYFENGKNVGVKPGLRYRILRPLTNFSG
                     DNAAAPDTVFARQVGLVEVEQVIEARSQAKIIWNSEAVLTTDLLQEEMVKWELISFNL
                     QSLPTNLGNPLMLEGGYGVEIPFKGFTRGNYALGLVNGIVIAQTGVEWGSEIPLKSGT
                     LYFFPFGGLGGSVALQNCEYSGSSELAFAKGLYATGGLGLKYYLHHEHGARIELAAVG
                     QLGPANYSWEGFDDDENTIDLTDWVPYSRFVGRGLTLRITVSIPLTGQTPTSRGFSAF
                     DDELTDGSY"
     gene            4241..5353
                     /locus_tag="VF531_12295"
     CDS             4241..5353
                     /locus_tag="VF531_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7714785.1"
                     /translation="MKKATVYFTNLRTSPGHNLLHKLEALVKKAGFEQLDLQDKLVAI
                     KIHFGEPGNLAYLRPNYAAQIVKLIKEAQGMPFLTDSNTLYTGKRANAVDHLQAAFEN
                     GFNPMATGCNVIIADGLRGYDYKDIEINCKNCKTAKIGTAIAEADVVISLNHFKGHEM
                     TGFGGAIKNLGMGSGSRGGKLDMHSGSKPQIDIKNCIACGKCIKNCSQGAIHFNAGKK
                     AEIDYEKCIGCGQCIFICRDDAAQVREGQSSDVSSEKIAEYAYAVVKDKPSFHVNFIM
                     NVSPDCDCFYLNDLPIVPDIGIAASFDPVALDRACVDLVNQAPVMKGSILDEKHYREG
                     EDKFTCIHPKTSWKVGLNHAEEIGLGTQEYELITIK"
BASE COUNT         1422 a         1372 c         1571 g         1416 t
ORIGIN      
        1 ggcgcgtaca tttgattacc gggccggtga tgtcggctat gtacccgttg gctatgtgca
       61 ctatattcaa aataccggta atgaaatcgt ctggttttta gaggcgttta agagcgatag
      121 gtttgaggga gtatcgttaa accagatgat ggcccttacc cctcccgggc tgattgcgag
      181 taatttgcag atcggaccgg aatttttgaa tgcattgcgc aaggtggatt gtccggtcgt
      241 tccttgttaa cgatgcgtca atggggtaaa attcgtatga ttcacacact gatccagtaa
      301 tcgccactat caaccgtggt tattctgtgt taaaattttt atgtcaacta actatcgacc
      361 cggatccgga aaatcgccgg atccgtattt tttgaccctt ggtccggcaa tcttgtaaag
      421 cgatttttag gagccgattc gggtaaaacg gtttggaacc gcttatacaa agcgaataaa
      481 aggttaccgg tatccttttt gtaaaggagg tagagaagat gatgaaacaa ctgaaaaata
      541 tgcaagcatg gttggtttta ttggccttta ttattacatt gtccgcgctt ttcggcgggc
      601 ggttgctctc caaccgattg caggtgacgg agccgttgcg gaaagagatg gccgcaatca
      661 aggcggttaa aaagtttaaa gtggatccgg agaacaacgg tttgaaggtc acactgaatc
      721 taggccgggt ggccaatctt caggatgtgc tggaacgggt tgaagaaaag gtggagcatt
      781 tctataacaa acctgtgaaa accattgttc tggtggatca ttccaatcaa cggctgcggc
      841 aggttcgtta tcaattagcg ttcaatcttg aggaggcgcg ggtttccggg cgttatctcc
      901 agctaaagtc ggctctcgaa tcttaccaag gtgtgaaggc tcaggtgtat ttcagcaagg
      961 attatttctt tatccagctg gaagacggat cctactatca ttatgaggca gtgccgcgcg
     1021 ggcagcaaac agttcaaccg acaggaggtg atcccgcatg atgcgtcgtt tcgaatggct
     1081 aatcggaatc ggtttgggag ttttagtcta tctgttttgg atcggcgtgg acttgacgcc
     1141 actcgttttg atcggcggtg cgctggggat cttatatttg ctcacccagg gccggttggg
     1201 ccggggcttt gaaacggtcg gcaataacaa aaccgtcacc cgggtggata ccggggtccg
     1261 gtttgacgat atcggaggcc aggaggtcgc caagcgtgaa ctcctggagg cactggattt
     1321 tgtgcgcgat caagcgaaga tcagtgccct cggcatccgg cccctcaagg ggattttgct
     1381 gaccggacct ccgggaaccg gcaagacctt gctcgccaag gcggcggcca attataccga
     1441 ctcggttttc gtctccgcat ccggttcgga gtttgtggag atgtatgccg gcgtcggtgc
     1501 gcaacgggtg cgccagcttt tcaacaaagc ccggcagttg gcttcaaaga tggccaagca
     1561 cagcgccatc gttttcatcg acgagattga ggtaattgcc ggaaaacggg gtaatcacac
     1621 gtcgcatctg gagtatgatc agactttgaa tcagttgctg gtagagatgg acgggatcaa
     1681 acccaatgaa acggttcaga tcgtggtcat cgccgccacc aaccgggcgg atctgctgga
     1741 tggggccatt ttacgccccg gacggttcga ccgtcaggtt aaggtagacc tgccggataa
     1801 ggaaggccgg atgcatatcc tgaatattca cgccaagggc aaacccttgg actccgagat
     1861 cgatctggaa gaggtggctc gtgatacctt cggattctcc ggagcgcatc tggagtcact
     1921 gatcaatgaa gccgccattc tggcgtaccg cagaggcaag agctgcattc agatggatga
     1981 tatcagggag gctatcgaca aggtgatgct cggcgagaag ctggaacacc ggcctgatcc
     2041 tgatgagctc aagcgggtgg cgatccacga ggtcggtcat gccatggtca gcgaggtgtt
     2101 gcggcctgat tcggtagcga ctctgactgt gattcccagg ggcaaggcgc ttggtttcat
     2161 gcgtcaggcc caggcgcagg atcattatct gtatacccgg gagtttctcc tggatcaggt
     2221 ggcgatctgc ctgggagggg ctatctccga agaattgata tacggcgatt ggagcaccgg
     2281 cgcctcgaac gatttcgagc aggccacttc tctcgccaaa cagatcatta ccaccgggct
     2341 ttcccggttg ggaatcatcc aggaggatct ggccggcaag gaccggatct atgaagtcat
     2401 gaccgagatc ctggaggagc aaaagtcccg ggtccgggag cttctgaaaa aacgcagccc
     2461 aatcataccg gccatcaccg agactttgct tgaacaagag aaggtttcgg gtgtctggtt
     2521 ccgtgaacaa ctgaaatgcg cgtgaagaac gtaacaaagc gggaaaacct tacgggtttt
     2581 cccgctttgt gatacataag tttcaataac tcccatccgt caattcatcg tcgaaagcgg
     2641 agaatccccg ggaagtcggc gtttggccgg ttagtggaat actgacagta atccgtaggg
     2701 tcaagccgcg acctacaaaa cgggaatagg ggacccagtc ggttagatcg atcgtatttt
     2761 cgtcatcatc gaatccctcc caggaataat tagcgggacc aagttgaccg actgcggcga
     2821 gttcaatcct ggcgccatgc tcatggtgta gatagtattt taaaccgagg cctccggtag
     2881 cgtatagacc tttggcaaaa gccagttcac tggagcccga atattcacag ttttggagtg
     2941 ccacgcttcc cccgagtccg ccaaaaggga aaaagtataa tgtcccggat ttcaggggga
     3001 tctcactgcc ccattccact ccggtctggg cgataacgat cccatttacc aaccccaatg
     3061 cgtaattacc ccgggtaaat cctttaaaag ggatttccac gccgtaacca ccttctaaca
     3121 tcaagggatt tcccaaattt gtcggaaggc tttggagatt aaagcttatt aactcccatt
     3181 tcaccatttc ctcctgaagc agatcggtgg tcagaaccgc ttccgagttc cagatgattt
     3241 tggcctggga tctggcttca atgacttgct ctacctccac cagaccgacc tgtcttgcga
     3301 agacggtatc gggggcggcg gcgttgtctc cggaaaaatt ggttaaaggc cggagaatcc
     3361 ggtagcgtaa ccccggtttt accccgacat ttttaccgtt ttcaaagtag atcgtatccc
     3421 cggagatgct ctggatggaa ctgtaaggag ccagaaattc ccggagttga gcttggaaac
     3481 ggtatccgaa acatttatcc agtgcggcat ggagggattc cttttcgccg ccgttggtac
     3541 tgacagcgga agcctgaatc accgacagaa tctcggctgt atctacagag atgatcttaa
     3601 tcgtaacttt tgctgtggcg gtatattctt taagatcttt atcatatgta gcggaaagtt
     3661 gatcgatatt tcctaagtaa gcggtggtaa tcccggcaat ccttcccgct tccagggcgg
     3721 tctcttgatc gacggcgccc gattcttgaa actcctgttc cttcaggata cgatccattt
     3781 ccgcccgttc cacgatttca tatttaccga aatgggacag gatcgaggtc agccggttgg
     3841 cggccctgat gcaatattcc ggatcgtcgg ctccgctaaa atccaggatt ccaatgcggt
     3901 cattctccac ggccctggcg ggaacgatat gccaggcaaa cagcaatata atgataccaa
     3961 gagatatcca aaattttttc atggaagttc ctccgctaaa cgttcaccca cgataatgat
     4021 ttcttttgga gaaggaaatt tcctcttttt gtagaaaaac gggaaaacca agatcgttcc
     4081 gattgtttat cgtccgcaaa agcgtttgaa aattcgggtt cgtggtaatc gtataatcga
     4141 tagtagtgaa ccgttctatg cggacagtgt caacttacgg gatagccccg ggcacagatc
     4201 ggatcgtttc atcaaaagct tcgaagggag tcaaggtacc atgaaaaagg caacggttta
     4261 ctttacaaat ctacggacca gtccgggtca taacctgttg cacaagcttg aggcgctggt
     4321 caagaaggcc ggattcgaac agcttgatct ccaggataaa ctggtcgcga tcaagattca
     4381 tttcggtgaa ccggggaatc ttgcttattt gcggcctaat tatgccgccc agatcgttaa
     4441 attgatcaaa gaggcgcagg gcatgccctt ccttaccgac agcaacacct tatataccgg
     4501 caaacgggcc aatgccgtgg atcatcttca ggcggccttt gaaaatggtt ttaatccgat
     4561 ggctactggt tgtaacgtga ttattgccga cggcttgagg ggttacgatt ataaggacat
     4621 tgaaatcaac tgtaagaact gcaaaaccgc aaaaattgga acggccatcg ccgaagcgga
     4681 cgtcgtcatc tccctcaacc atttcaaggg tcacgagatg accggtttcg ggggcgcaat
     4741 caaaaatctc ggcatgggtt ccggttcccg cggggggaaa ttggacatgc attccggctc
     4801 caaaccgcag atcgatatca agaattgcat agcctgcggc aagtgcatca agaactgttc
     4861 ccagggagcc attcatttca atgccggtaa aaaggcggag attgattatg agaagtgcat
     4921 cggctgcggc caatgcatct ttatctgccg tgacgacgcg gctcaggtgc gagagggcca
     4981 atcgtcggat gtctcaagcg agaagatcgc cgaatacgcc tatgcggtag tgaaggataa
     5041 acccagtttc cacgtgaact tcatcatgaa tgtctccccg gactgcgatt gtttctatct
     5101 gaacgacttg ccgatcgttc ccgatatcgg tatcgcggct tccttcgatc cggtcgccct
     5161 cgaccgggcc tgtgtggatc tggttaatca ggcgccggtg atgaagggtt ccatcctgga
     5221 cgaaaagcat taccgggaag gtgaggacaa gttcacctgc atccatccca aaaccagctg
     5281 gaaggtcggc ctcaatcatg ccgaggagat tgggctcggg actcaggagt atgagttgat
     5341 aacgataaag tagcttaagc gcggtttaac ccctcgcccg tgataatggg cgaggggtta
     5401 tattttttta cctcaagggt gattgattcg gattatgtat tttttcatat gtttcttact
     5461 gtaacatagg ggctgcagcg cgaaccggat ggtccctttg gggcaggtat cgacacaaga
     5521 gccgcagaga atgcattctg aatggttgat tactccagtt ttaacgagag tgtccaccgg
     5581 gagagacatt ggacattttc gtgcacattg cccacaggag ttacatgatt ccggggaagt
     5641 ccttaagtga agcgccggaa gtttgagtcg ggcgcgaatt gcccctccga tgatcataaa
     5701 tggggatatc cagcaaagat aatggcaaaa acctcgcttc cccgtagcta aggtgagaat
     5761 aaggatgaac gcaacggtca t
//
LOCUS       DASVMB010000438         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_494790,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000438 DASVMB010000000
VERSION     DASVMB010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494790"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2518)
                     /locus_tag="VF531_12300"
     CDS             complement(<1..2518)
                     /locus_tag="VF531_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX7714786.1"
                     /translation="MEQYGFPNQQGLYDPRYEHDACGIGMVVNVKGTPSHEILDQGLT
                     VLLNLDHRGARGAEANTGDGAGVLLQLPYKFFHKVSTGLGFELPAPGDYAVGMIFLSQ
                     DPSRRAECEKLFARFVHEEGLVMLGWRTVPTCNATLGDSAKECQPYIRQAIIGRSPAI
                     ESGLAFERKLYVIRKRAEKAIRYSGVNFGKSFYVASLSSRTIVYKGMLTPEQLRDFYP
                     DLSDPDMETALILVHSRFSTNTFPSWERAHPNRYLIHNGEINTLRGNINWMHARESIL
                     RSEQFGEDIHKVLPVISSDGSDSSMFDECLEFLSLTGRTLPHAMMMMIPEPWSKHETM
                     SPSKKAFYEYHSCLMEPWDGPAAMAFSDGQIVGAVLDRNGLRPARYYVTKDDLVILAS
                     EVGVIQVPPERILEKGRLKPGRMLLIDTRAGRIISDEELKSSLVSEQPYREWLDRYLL
                     SLNRLPEAPLPPEETETLTAREKIFGYTYEDLRQILTPMARDGVEPVGSMGTDIPLAV
                     LSDQPQLLYNYFKQLFAQVTNPPIDALREEIVTGTETMLGSEGNLLDPQPESCLQLKL
                     ANPILSNEELAKIARLNRPGFKTVTLPILFPVSSDGSGLLTAMKTLCQQAHEGIKAGA
                     NILILSDRGVSREMAPIPALLGVSGLHHYLIREQLRTQVSLVVESGEPREVHHFALLL
                     GYGASAINPYLAYEAVSDLARRNMLNDCPDEHKAVKHYTKAVIKGIVKILSKMGISTI
                     QSYQGAQIFEAIGLNETLIDQYFTGTPSRIGGIGLEAIAREAQLRHEQAFTTIQPDNR
                     VLEPGGVYQWRKNGEHHLYNPETIYKLQQACINNDYQLYKE"
     gene            complement(2771..2980)
                     /locus_tag="VF531_12305"
     CDS             complement(2771..2980)
                     /locus_tag="VF531_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714787.1"
                     /translation="MHLTAKQRRSLIIGLSLFLLGANLIQAIFRFNINRRLFDNLNYI
                     LMFIVIGLALTGRKPPKDPGSDRKS"
     gene            complement(3017..3985)
                     /locus_tag="VF531_12310"
     CDS             complement(3017..3985)
                     /locus_tag="VF531_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7714788.1"
                     /translation="MQPINLGIIGTGIAARDLHWPALRRMPEKFTIKAVCNHTEEKAK
                     EFSRMVGGVPYVTDYRQLLEMPEIDAVDITLPIELNYEVVKAAMQAGKDLIVEKPLAA
                     SLPQAEELVELEKDFARVAMVAEQFRYRSRFQRIKTLLGEGRIGEPEAVFWNGFTRTD
                     PTNKYAQTKWRLHHQYPGGFVTDGGVHNVAALREILGEITVIGAFTKSFNPSIGQMDS
                     ISLQFTTSSRVSGVLNLFFGATGYTENRLIILGKKGAITLENDRLTVSGDNQTAMTEE
                     FPDDLGFREELEDFYQSVRTGRPVKSSFKEAYLDLKVILHALAMAE"
     gene            complement(4153..>4621)
                     /locus_tag="VF531_12315"
     CDS             complement(4153..>4621)
                     /locus_tag="VF531_12315"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEX7714789.1"
                     /translation="PFVEAAFSFLEKEYQTKVTKGTLNLAQSPFPGKDVNVVIGLSGS
                     LRGQVIFCLSIETARQIASRMLCGMQVDDFDEIAKSAISEMGNIISGTATTILSQTGI
                     SCIITPPSLLLGQGMSISFKDMQILVVPLSTDIGEITVLVAVKATEENPAQSL"
BASE COUNT         1092 a         1170 c         1225 g         1134 t
ORIGIN      
        1 actccttata cagttgataa tcattgttga tgcatgcttg ctggagttta tagatggtct
       61 ccggattata gaggtgatgc tcgccgtttt tacgccactg ataaaccccg ccgggttcca
      121 aaacccggtt atccggttgg atcgtcgtaa aagcctgttc gtggcggagt tgcgcctcac
      181 gggcaatggc ctcaagaccg attcctccga ttcgggaagg tgttccggtg aaatactgat
      241 cgattaacgt ttcatttaag ccgatggcct cgaagatttg agctccctga taactttgaa
      301 tggtggagat gcccatcttg gagagaattt tgacgattcc tttaatcacc gctttggtgt
      361 aatgcttcac cgccttgtgt tcatcggggc aatcattaag catattccgg cgggccaggt
      421 cggatactgc ctcgtaggcc aggtaaggat tgatggctga ggcaccataa ccgagcagga
      481 gtgcaaaatg atgaacctcc cggggttcgc ccgactccac taccaggcta acctgggtgc
      541 gtagctgttc acggatgaga taatggtgga gtccggaaac cccaagcaat gcagggattg
      601 gagccatttc acggctgact ccccggtcgg aaaggatcag aatattggct ccagccttaa
      661 tcccctcatg cgcctgttga cagagagttt tcatcgccgt caggagtccc gaaccgtcac
      721 tggatactgg gaagaggatc ggtagggtta ccgtcttgaa cccaggccga ttcagccggg
      781 cgatctttgc cagttcctca ttggagagga tcggattggc cagcttcagc tggaggcaac
      841 tttccggttg cggatcgagt agattaccct cagaacccaa catcgtctcg gtaccggtga
      901 caatctcttc acgtaaggcg tcaatcggcg gattggtgac ctgggcaaag agttgcttaa
      961 aatagttata gagtaactgg ggctgatcag agaggaccgc cagtggaatg tcggttccca
     1021 tagatccgac cggttcaacc ccatcccggg ccattggggt caatatttgc cgcagatctt
     1081 cataggtata gccgaagatc ttctcccgag cagtcaaggt ctcggtctct tcgggtggta
     1141 gcggcgcttc cgggagccta tttagggaga gcaggtaacg gtcaagccat tcccggtagg
     1201 gttgttctga gaccaagctg cttttgagtt cctcatcgct aatgatccgg ccggcccggg
     1261 tgtcgatgag cagcattctt ccgggtttga gacgcccttt ctcaaggatt cgctccggcg
     1321 gcacctgaat gacaccgacc tcggaagcca ggatcaccag atcgtccttg gtcacatagt
     1381 accgtgcggg gcgaagtccg ttccgatcca gaactgcgcc gacaatttgt ccatcggaaa
     1441 atgccatggc cgccgggccg tcccagggtt ccatcagaca actatggtat tcgtagaaag
     1501 ccttctttga cgggctcatg gtctcatgct tcgaccacgg ctcgggaatc atcatcatca
     1561 tggcgtgggg caatgtccgc ccggtcagag aaagaaattc caaacattca tcgaacatcg
     1621 acgagtcgct cccgtcgcta ctaatcaccg gcaagacttt gtgaatatcc tcaccgaact
     1681 gctccgaccg gagtatggac tcccgggcat gcatccaatt gatattgccg cgtagagtgt
     1741 tgatttcacc gttatgaatc agatagcggt tgggatgagc ccgttcccag ctgggaaagg
     1801 tattggtgct gaaacgggag tggaccagga taagtgcggt ttccatatcg ggatcggaga
     1861 gatcgggata aaaatcgcgc aactgttccg gtgtgagcat tcccttgtag acaatggtcc
     1921 ggcttgagag gctcgccacg tagaaagatt taccgaaatt caccccggag taacggatgg
     1981 ctttttcggc ccgtttccgg atgacataca gtttgcgttc aaaggctaag ccgctttcga
     2041 tggcgggact ccggccgata attgcttgcc ggatataggg ttgacattct ttagcggaat
     2101 cgccaagagt tgcgttgcag gtgggtacgg tacgccaccc cagcatcaca aggccctcct
     2161 catgtacaaa acgggcgaaa agcttctcac actccgcacg tctcgatgga tcctgagaga
     2221 ggaagatcat tcccactgcg taatccccgg gggcgggaag ctcaaaaccc aatccggtgc
     2281 tgactttatg aaagaattta taaggcagtt ggagaagcac accggcgccg tcgcctgtat
     2341 tggcctcggc tccccgggca ccgcgatggt ccaaatttag aaggacagtg aggccttgat
     2401 caaggatctc atgggatgga gttcctttca cattcacgac cattccgata ccgcacgcgt
     2461 catgttcata ccgggggtca taaagacctt gttgatttgg gaaaccatac tgttccatga
     2521 tcttcacctt tctcgctacg gatacaaaaa ttccggaacg aatcccattt tccgtataac
     2581 ggaattcatt tccgtaaatt atattttatt cgttctccaa caagactgtc aacatttcgt
     2641 atggatgaat acaatattct tttgaaaaag tttttggatc cgcgggaagg gagcgcatgc
     2701 gcggggataa ggaataaccc cctgttcggg ggttattcct caaacagagg gttatttcac
     2761 tggcgaggag tcaagacttc cggtcggaac cggggtcctt gggaggttta cgcccggtca
     2821 gtgcaaggcc gatcacgatg aacatcagga tgtagttcag attgtcaaaa agccggcgat
     2881 tgatgttgaa gcggaaaatg gcttggatca agttggcgcc cagtaaaaac aggctgagcc
     2941 cgataatgag cgaacggcgt tgtttggcgg tcagatgcat cagggaagtc cgcctccttg
     3001 gaatcccttg ggaatattac tcggccatag ccagggcatg gagaattacc ttcagatcga
     3061 ggtaagcctc tttaaagctg cttttcacag ggcggccggt acggacggat tgataaaagt
     3121 cctccagttc ttcgcgaaag cccagatcat ccggaaactc ctcggtcata gcggtttgat
     3181 tgtcaccaga gacggttaag cgatcattct cgagtgtaat ggcacctttt tttcccagga
     3241 tgatcaagcg gttctcggtg taacccgtgg ccccaaaaaa caggttcaat acaccgctga
     3301 cccgggaaga ggtagtaaat tggaggctga tcgagtccat ttggccgatg gacgggttaa
     3361 aacttttcgt aaaggcgccg atcacggtaa tctcacctaa aatttcccgt aaagcggcga
     3421 cattgtggac gccgccatcg gtgacaaaac cgcccggata ctggtgatga aggcgccatt
     3481 tggtctgggc gtatttgttg gtaggatccg tccgtgtgaa gccattccaa aatacggctt
     3541 cgggttcacc gatccgtccc tcgccgagta gagtcttgat ccgttgaaac cgggaacggt
     3601 agcggaattg ttcggcaacc attgccaccc gggcaaagtc tttttccaat tccaccaatt
     3661 cttcggcctg gggcaggctg gctgccagag gcttttctac aattaggtcc ttgcctgcct
     3721 gcatcgccgc tttgaccact tcatagttca actcgatagg cagggtaata tcgaccgcgt
     3781 caatctccgg catttccaag agttggcggt agtccgtaac atatgggacc cctcccacca
     3841 tacgggagaa ctccttcgcc ttctcctcgg tgtgattgca gactgcctta atggtaaact
     3901 tctccggcat ccgacgtaaa gccggccagt gcagatcgcg ggcggcgata ccggtaccga
     3961 tgatccccaa gttaattggt tgcataacaa acgcccctct ccgaagatta ccatggaatg
     4021 gttcgttttt gaaaccgaat cgcatggtta gttgaatact taactatttt accattccga
     4081 ccatcccagt caacggagct aatcaggaaa agttccattt tcgaagaagg ataggttcga
     4141 tatacttgat ggttacaagc tttgtgcagg gttttcctca gtcgccttga ctgccactaa
     4201 caccgttatc tcaccgatat cggtgcttaa aggaaccacc agtatctgca tgtctttaaa
     4261 ggaaatactc atcccctggc ccaatagcaa cgagggcggt gtaataatgc aggaaatacc
     4321 cgtttgactt agaatggtgg tggctgtacc gctgatgata ttgcccatct cgctgatggc
     4381 gctcttggcg atctcatcga agtcgtctac ctgcattcca cagagcatcc gcgatgcgat
     4441 ctgtcgggcc gtctctatgg ataggcagaa gatgacctga ccccggagag aaccggatag
     4501 tccgatcaca acattgacat ccttcccagg aaacggtgac tgcgctagat tgagtgtacc
     4561 tttagtcacc tttgtctggt actctttttc aagaaatgag aaggcggctt ctacaaatgg
     4621 a
//
LOCUS       DASVMB010000439        12266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_423193,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000439 DASVMB010000000
VERSION     DASVMB010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423193"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VF531_12320"
     CDS             <1..1757
                     /locus_tag="VF531_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7714790.1"
                     /translation="GLGSLRSKFILCFMVLGIIPMILLAFVSYRVYLDILQQNVQTYT
                     STVIDRVDRSLEIYLSDLERILELRNDYYHVQFIKLSLAGDIEGNRKYTFRLWENLNN
                     IKQFKTDLRDVAITTSAGVSVGCYGVTHIDRGLNPLFQTLARQPASRDGMRIWEPHPD
                     WLGGKVFTVGRAIRGDYDNFLGMMSIDVDVELLDRICRNIRLGKTGYVFLADSTDRII
                     YHPQPEYIGKPLTSLLGKSATSEWQPGFFSSKTGPEYRVITIKEFYPANWRIIGLSSQ
                     NELTAAMQKVTGVSLALIIASIAVLTLAAIALAGLLTRPMKKLQQSMRAAAEDLTTNV
                     RIGTHDEIGELGEAFNQMIIRIRRLMEESVREQKKRRQTEMIALQEQIKPHFIYNTLD
                     LIIGLLETNKNEDVINMVEALGAFFRISLSHGQEFIAIRDEVEHVRNYLYIQRFRHGD
                     GYDYEITMEERVLDKKTIKLILQPLVENAIYHGVRELEHPGGLITVVGYCEGEHVSIK
                     VIDNGKGMDGATVTEINRCLNGAAVSDERRFFGLRNVNDRIQLAFGKDYGLRLESAVD
                     QGTVVTVHLPLIVNKPKE"
     gene            1771..3384
                     /locus_tag="VF531_12325"
     CDS             1771..3384
                     /locus_tag="VF531_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7714791.1"
                     /translation="MTGLLMVEDEERIQQKLLENVSWKEFGFDPVLGAGNGLEALDLL
                     RDYSIDIIVTDIQMPKMNGIELIKALKADGYQGKIVVISGFAEFEYAQESIKLNVADY
                     LLKPFTSKKLLETVTRLKNQLEQERAEKSELLLLREQLHKNREVLREKFFSNLLNHNW
                     NRSTSGDELNFLGLGDLESKPCQVAVVEIPENLLHQTEEEKYLINLMLLTQMKRIFAD
                     SGYNHFLVNQLRNQVAAIFYEPDQNLGLRLEEFLNQLQLSLKQPLVIGVGNTYHSLGD
                     LSASYREACMALQYRYLHGPNRVFSIRDLNLDNPFYHKQFYYLHQNRIFDDLRIGAAG
                     AIRDDVAAFIGILKNSSMPPESLRTLASNLILLTYATLNELGYHPVEIFGSAFSPLTD
                     VNRTESLGELEEFLLMFFDRINRYTERKRTSLNQTLVDEIRCQIDNQYAAEITLSGMA
                     DRYKISPGYLSLLFTERTGKNFIDYLTERRIKRAKELLKHTAMKIYEIAAEVGYNDSY
                     YFSNCFKKVVGLTPTEYREHMEGGEAYPP"
     gene            3638..5116
                     /locus_tag="VF531_12330"
     CDS             3638..5116
                     /locus_tag="VF531_12330"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7714792.1"
                     /translation="MTELLRMNKICKRFPGVVALSQVDLAVAGGEILALMGENGAGKS
                     TLIKILAGVYVKDSGEIFFEGRRVDITSPHESQLLGISVIFQELNLLPNLSVAENIFM
                     GRENKRLGGFLDFGATRRLAGDLMERVGLHCSPETPVHSLPVSQRQMVEVAKALSLKA
                     KLIVMDEPTSSLTEGETQLLFGIIRTLKASGVAVIFISHRLNEVFTIADRVQVLRDGA
                     NAGGLERNDLNEAAIIRLMVGRELKNIFAKEPVPIGERVFRVNHLSSPALHDINFELR
                     KGEILGIAGLVGAGRTELIRAIFGLDRRDRGEIYINGQPVAIKTPEQAIRRGIAFVTE
                     DRRNQGLILGMSVRENCTLVALRDLQAGGFIRFHKERKTVREAIDLLKIKTPSMETVT
                     ATLSGGNQQKVVLAKWLVINPRVLILDEPTRGIDVGAKAEIHRIMSDLARQGVAIIMI
                     SSELPEILGMSDRIIVMHQGRLTGELSREEATQERIMALAIR"
     gene            5146..6219
                     /locus_tag="VF531_12335"
     CDS             5146..6219
                     /locus_tag="VF531_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714793.1"
                     /translation="MRQNNALTQPGSANRTPDRRSNGGLIASRWHRLIQSQIIGILSI
                     LLILCLVLWITCPTTFPTTDNLFSVARQFSYIAVAAIGELMVIITGGIDLSVGSIMGF
                     GAVLTGFLMHNLNYPIPLAIGFGIIGGAILGLFNSFCITKMNLPPFIATLGTMSIGRG
                     LAYAITEGYTVPVPPLFARLGQGYLGPIPYPVIDMIVIAIIFAIFLGRTVLGRRIYAL
                     GGNEEAAKVSGINVSRLKVFVYMFAGILAAMGGIICAARLGVAQSTLALGYELDVIAA
                     VFIGGASTTGGSGTVLGTILGAAIMGIIRNGLVLLNVSPYWVQTAIGTVIIIAVAFDR
                     FRLVYQEKKQLREVIVRENTSEV"
     gene            6262..7236
                     /locus_tag="VF531_12340"
     CDS             6262..7236
                     /locus_tag="VF531_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX7714794.1"
                     /translation="MRKTFGLFLGMLVLMIGLFSAVALQASEKPTIAMIPKSLDNPVF
                     LDAKTGGEKTAKELGVNFIWTGSNTADAANEVTIIEGLIQKKVDGLIISCNEAGALKD
                     VIDRATAAGIKVATFDSDSPQSKRVFYCGTDNYKAGKACGAAMVKLIRERKLAGKTLT
                     CALLTGGLAAPNLNDRIKGFKEVVAAEKINLKYLNTLACDDDTNRAVELMEQYIKANP
                     KLDTFFVVGGWPFFSAPGSMPNIKAWVKKGGILVSMDTFYPVMVAMKDGLANALVGQN
                     FTAMGELSVRTMVDLLNGKNVTEFIDTGIVNVDKSNFDKVFAETIPWK"
     gene            complement(7365..9032)
                     /locus_tag="VF531_12345"
     CDS             complement(7365..9032)
                     /locus_tag="VF531_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7714795.1"
                     /translation="MQGLHLKYVNETYDKKGMLKAFSLNVPADFNFSFDVVDEMARLE
                     PNKKALVWCDDTGAERIFTFAEIKKYSDQTANFFKSLGIGKGDMVMLILKRHYEFWFS
                     IVALHKLGAVAIPATHLLTTKDLVYRFNTAGVKAVVCTAMGEITNYVDEAQAECPSIT
                     IKIIARGKKDGWHSFTENMEAAPENFERPRGAQKQKVSDPMLIYFSSGTSGLPKMACH
                     NFAYPLGHITTAKHWQNVDPDGLHLTVADTGWGKAVWGKLYGQWLMEAAVFVYDFDKF
                     VPADLLAKIEKYKITTFCAPPTIYRFFIKEGIEGFDLSSLQYATVAGEALNPEVFNKF
                     HHYTGLKLMEGFGQTETTLTVLNWKGMEPKPGSMGKPSPQYEISIVDENCQPVPTGEV
                     GEIVVCTKHGKPAGLFSGYYNDKEKTDDVWHDGYYHTGDTAWQDEDGYLWYVGRTDDV
                     IKSSGYRISPFEIESVLIEHPAVLECAVTAAPDPVRGQVVKATIVLTRNYTASGELAE
                     EIKDYVKQATAPYKYPRIIEFVSELPKTINGKIRRVELREKSKQTGK"
     gene            complement(9135..9719)
                     /locus_tag="VF531_12350"
     CDS             complement(9135..9719)
                     /locus_tag="VF531_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7714796.1"
                     /translation="METHILEISERIKGMREILNITAEEMADVTGTTIDDYLAAENGN
                     SDFTFTFLMKCAQRFKVDIVELLTGSNPTLSFYTVVRKGGGLPIERRKGFDYKHLAYR
                     LKDKMVEPFLVTVPYNQKEQNTPLYYSTHAGQEFDYILKGSLKVDLDGHVEILNAGDS
                     VLYDSGHPHGMTAMNGADCQFLAIVIPKPKEKNQ"
     gene            complement(10274..11077)
                     /locus_tag="VF531_12355"
     CDS             complement(10274..11077)
                     /locus_tag="VF531_12355"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7714797.1"
                     /translation="MNEVYKVITPENVELDYEVAGIGSRFLAIVIDTLIQWVLIIGIL
                     SSLGMLKLGELRSKITHWSTSFAGSLLIGLLFLVLIGYFIILETVMNGQTVGKKLVNI
                     RVRKEGGYAPSFWDVLLRNVIRMVDYLPFFYGFGMITMFFNQKAKRLGDYAAGTIVVK
                     ELPRRKVKKFLDEQAAQTVVSPGESTAVIERYPWLPGIVPSITQADYLIMKNLYSRQK
                     ELTTGRSLAKTLIQKVVSASIKAEDLSINESDIMEVLAEMIVLYENQQF"
     gene            11130..12122
                     /locus_tag="VF531_12360"
     CDS             11130..12122
                     /locus_tag="VF531_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX7714798.1"
                     /translation="MAIPFVQARQERWIRLQNLVKKASGRKLTSFSKEELIELSVLYR
                     QTATDLALAKTQGLPDEITSFLNDLTTNSYHYIYKTEKSTLRQLGDFIVHEFPELFRE
                     NWRVILFAFSLLFAGWAAGFWGYIAGPDFIAGLLPEKFTTGIFSGYQEKTWFNEPLVA
                     RPYVSSVIMNNNIQVAIQAFGGGMLLGTLTCWAILFNGFILGVLAAVFFQKGYLLSFW
                     AMILPHGVIELTAIGLSAAAGLLLTRAILFPGDYSRSDALKTCGIKAVKLLAGTICML
                     IVAGLIEGFFSTISTDLIPESVRLVFAAVTAILLFLYFKPRLLFHRKTDRSDGS"
BASE COUNT         3072 a         3063 c         2988 g         3143 t
ORIGIN      
        1 cgggattagg atcgctccgt tccaaattca tcctgtgttt tatggtcctc ggaatcatcc
       61 ctatgatttt actggccttc gtttcatacc gggtttacct cgatatcctt cagcaaaatg
      121 tccagaccta taccagtacg gttattgatc gcgtcgatcg ttcccttgag atttatctca
      181 gtgatctgga acgtattttg gaactgcgca acgactacta tcatgttcaa tttatcaaat
      241 tgagcctggc cggggatatt gaaggcaacc gtaaatacac cttccgccta tgggaaaacc
      301 taaacaatat caaacaattc aagaccgatc tccgggacgt ggcgatcact acttcggcag
      361 gggtcagcgt gggttgttac ggcgtgaccc atattgatcg cgggctgaat ccgctctttc
      421 aaaccctggc ccggcagccc gcttcccggg acgggatgcg gatctgggaa cctcacccgg
      481 attggcttgg aggtaaagtt ttcacagtcg gccgggccat ccggggggat tatgacaatt
      541 tcctggggat gatgagcatc gacgtggatg tcgaactgtt ggaccgaatc tgccgcaaca
      601 ttcgtctggg aaaaaccggc tatgttttcc tggccgacag cacggatcga atcatctacc
      661 accctcaacc ggaatatatc ggaaagcctt taacctccct cttgggaaaa tccgcaacca
      721 gcgagtggca gcccggattt ttctcatcaa aaaccgggcc ggagtaccgg gtaatcacca
      781 tcaaagaatt ctatccggca aactggagga ttatcgggct ctccagtcag aatgaattga
      841 ccgcggcaat gcagaaggtt acgggagttt cactggcatt gatcatcgcc tccatcgccg
      901 tgctgacact ggcagcgata gcattggcag gcctccttac cagacccatg aagaaactgc
      961 aacagtccat gcgcgccgct gcggaggatt tgaccaccaa tgtccggatt gggacccatg
     1021 atgaaatcgg ggaactcggt gaggccttca atcaaatgat catacggatc cggcggttga
     1081 tggaggaaag tgtccgggaa caaaaaaagc gcaggcagac ggagatgatc gcactccagg
     1141 aacagattaa accccatttt atctataaca ccctggatct gatcatcggg ctcttagaga
     1201 ccaataaaaa cgaggatgtt attaatatgg tcgaggccct gggcgccttt ttccggatca
     1261 gtctcagcca cgggcaggag tttatcgcca tccgcgatga agtggagcat gttcgaaatt
     1321 acctctatat tcagcgattc cgtcacgggg atggatatga ttatgaaatc accatggaag
     1381 agcgggtttt agataaaaag accatcaaac tgattctcca gcccttggtg gaaaacgcca
     1441 tttaccacgg tgtccgggaa ttggagcacc ccggcggact gattacagtc gtaggttact
     1501 gtgaggggga gcatgtttct atcaaggtga tcgacaacgg caagggaatg gacggagcga
     1561 cagtgacgga gatcaaccgc tgcctgaatg gagctgcggt cagtgacgaa cgacgttttt
     1621 tcggtttgcg caatgtcaac gaccgtatcc agctggcttt cggaaaggat tacggactgc
     1681 ggctggaaag tgcggtcgat caaggtacag tggtcacggt tcatctgccg ctgatcgtga
     1741 ataaaccaaa agagtgaaag aagggattac atgaccggcc tgctcatggt ggaagatgaa
     1801 gaacggatcc agcaaaaact tctggaaaac gtctcctgga aggaattcgg tttcgatccg
     1861 gttcttggtg ccggtaacgg cctcgaagcc cttgatctac tccgggacta ttccatcgac
     1921 attatagtaa ccgacattca gatgcccaag atgaatggga ttgaactcat taaggctctc
     1981 aaggccgacg gttaccaggg aaaaatcgtc gttatctcgg gcttcgccga atttgaatac
     2041 gcccaggaat ccatcaaatt gaatgtcgcg gactatctct taaaaccatt caccagcaaa
     2101 aaactcctgg agaccgtgac ccgtttaaaa aaccaattgg aacaggagcg ggctgaaaaa
     2161 tccgaacttc tcttgctcag ggagcagctc cataaaaacc gcgaggtttt acgggaaaaa
     2221 ttctttagta atcttttaaa ccataattgg aaccgctcca ctagcggcga tgaactaaac
     2281 tttcttggcc tcggggattt ggagagcaaa ccgtgccagg tggcggtggt ggaaattccc
     2341 gaaaacttgc tgcatcagac cgaggaggag aagtacctca tcaacctgat gctcctgact
     2401 cagatgaagc ggatatttgc ggatagcggc tataaccatt tccttgttaa ccaactccgc
     2461 aaccaagtgg ctgcgatctt ctacgaaccc gatcagaacc tggggctaag gttggaggaa
     2521 tttctcaatc aactgcagct ttccttgaag caacccctgg ttatcggcgt cggaaatact
     2581 tatcatagcc tcggggatct ctccgcttct taccgtgaag cctgtatggc attgcaatac
     2641 cgttatctgc acggtcccaa ccgggtgttt tccattcgcg atctcaatct ggataatccg
     2701 ttttatcata aacaattcta ctatctgcac caaaaccgga tcttcgacga tttacgcatc
     2761 ggagccgccg gagcaatccg ggatgatgta gccgctttta tcgggatact aaaaaactcc
     2821 agtatgcctc cggaatcctt gcgtactctc gccagtaact tgatcctgct gacttatgcc
     2881 actctcaatg aattaggtta tcacccggtt gagattttcg gaagcgcttt ctcccctttg
     2941 accgatgtaa accgcaccga aagcctgggg gaacttgaag aatttctgtt gatgtttttc
     3001 gaccggataa atcgatatac ggagcggaaa cgcacctcgc tcaatcaaac tctggtagat
     3061 gagatccgat gccagatcga caatcaatac gccgctgaga tcaccctgtc cggaatggcc
     3121 gaccgctata agatcagtcc cggttattta agtttgctat ttacggagcg caccggcaag
     3181 aatttcatcg attacctcac cgaacgacgt atcaagcggg ccaaggagct tctcaagcat
     3241 accgcgatga agatctatga gatcgcggct gaggtgggtt acaacgattc gtattatttc
     3301 agtaactgtt ttaaaaaggt tgtgggtctt acgcctaccg agtaccggga acatatggag
     3361 ggaggcgaag catatccccc ataatcccat ataatccagg cattctccca cccgtatttt
     3421 ccaagaagat gtaaccccgt gctgtgccat caaatcttcc cttttttatt cattttccaa
     3481 ggccgtttct tatttaccga ggagaaaaaa tatcgcagta aaataaaaaa tcctccattc
     3541 aaatccgctc cccggttttc tatactgggt gtgagaggtt aaattttcca tcctctctca
     3601 agaagtctca aagtgctgcc tctcaggagg tgttgtcatg actgaactct tgcggatgaa
     3661 caagatctgt aaacgtttcc cgggagtagt cgccctcagt caggtggatc tcgctgtagc
     3721 tggcggcgaa atcctggcgt tgatgggcga gaacggcgcg ggaaagtcga ctctcatcaa
     3781 aatcctggcc ggcgtttacg tgaaagacag cggtgaaatc ttttttgaag gcagaagagt
     3841 ggatatcacc tcgccccatg aatcccaatt actgggtatc agtgtgatct ttcaggagct
     3901 aaatctcctt cccaacttaa gtgttgcgga gaacatcttt atgggccgcg agaataagcg
     3961 tttgggtgga tttctggatt ttggagccac ccggagactg gcaggggact taatggaacg
     4021 cgtgggcctg cattgttccc cggaaacccc ggtccatagt ctaccggttt cccagagaca
     4081 gatggtggaa gtggccaaag ccctttcact aaaggccaaa ttgattgtga tggacgaacc
     4141 cacctcatcg ctgacagagg gtgaaaccca gttgttgttt gggatcatcc gcaccctgaa
     4201 ggcatcgggt gtagcggtaa tcttcatctc ccaccgccta aacgaggtct ttaccatcgc
     4261 cgaccgggtc caggtcttac gggacggagc caatgccggc ggactggaac gtaacgactt
     4321 gaatgaggcg gcaatcatcc gcctgatggt cggcagggaa ctaaagaata tcttcgccaa
     4381 ggaaccggtt cccatcggag aacgggtctt tcgggtcaac catctttcat cccccgcatt
     4441 gcatgatatc aacttcgagc tacgaaaggg agagatactg ggaatcgccg gcctggttgg
     4501 agcgggccgc accgaactca tccgggcaat tttcggcctg gaccggcgtg accgcgggga
     4561 gatttatatc aacgggcaac cggtggcgat caaaacaccg gaacaagcga tccggcgtgg
     4621 aatcgccttt gtgaccgagg atcgccgcaa tcagggtttg attttgggaa tgtcggtccg
     4681 ggaaaattgc accttggttg ctttacggga tttacaagcc ggaggcttca tccgattcca
     4741 caaagaacgg aagaccgtcc gggaagccat cgacctgctc aagatcaaaa caccctccat
     4801 ggaaactgta accgccacat tgagcggggg gaaccagcaa aaggtggtcc tggcgaaatg
     4861 gcttgttatc aacccccgag tactgatcct cgatgaaccg acccggggaa tcgacgtcgg
     4921 cgccaaggct gagatccacc ggatcatgtc cgatctggcc cggcagggag tggcgatcat
     4981 catgatcagt tcggaacttc cggagatcct ggggatgagc gaccggatca tcgtcatgca
     5041 ccagggcaga ctcaccggcg agttaagccg tgaagaggcc acccaggaga ggattatggc
     5101 tctggccatc agatagaaga tggaaaaaca aaggagggtc gcgccatgcg gcaaaataat
     5161 gctctaaccc aacccgggtc tgcgaatcga accccggacc ggcgaagcaa cggaggactc
     5221 atagcttcac ggtggcacag gcttatccaa tcgcagatca tcgggatcct ttcgattctg
     5281 ctcattctct gcctggtgct ttggattacc tgcccgacca cgttccccac aacagacaac
     5341 ctcttctccg ttgcacgcca attttcctat attgcggtgg cagccatcgg ggaattgatg
     5401 gtcatcatca ccggcgggat tgatctctcg gtaggttcga tcatggggtt cggagcggta
     5461 ctcaccggtt tcctaatgca caatctgaat tatcccattc ccctggcgat tggatttgga
     5521 atcatcggcg gcgccatctt gggattattc aactcctttt gtatcaccaa gatgaatttg
     5581 cctccgttta ttgccaccct cggaacaatg agtatcggcc gggggttggc gtacgccatc
     5641 accgaaggtt acaccgttcc ggttcccccg ctattcgcca gacttggcca aggctacctg
     5701 ggccccattc cttacccggt catcgacatg atcgtcatcg caatcatttt tgcgatcttt
     5761 ctgggccgga ctgtactggg acggcgtatt tatgccttgg ggggaaatga ggaggccgcc
     5821 aaggtatccg ggatcaatgt ctcccggttg aaagttttcg tctatatgtt cgccggtatc
     5881 ctggcggcga tgggcgggat catctgtgcg gcccgtttgg gagtcgccca gtctacattg
     5941 gcccttggat atgagctgga cgtcatcgca gcggtcttta tcggtggagc cagcaccacc
     6001 ggcggttccg gaacggtact tgggaccatt ctcggcgcgg ccatcatggg aatcatccgt
     6061 aacggattgg tgcttttaaa cgtcagccct tattgggtgc aaaccgccat cggcactgtc
     6121 atcatcattg cagtggcttt cgatagattt cgcctggtgt accaggaaaa aaagcaattg
     6181 cgggaggtga tcgtcaggga gaacaccagt gaggtttgat tttccagcgc ggatcggtta
     6241 tcaaacgaag gaggaaacga aatgagaaag acgtttggat tattcctggg tatgctggtg
     6301 ttgatgattg gactgttcag tgcagttgcg cttcaggcat ccgaaaaacc gaccatcgcc
     6361 atgatcccga aatcccttga caacccggtt ttccttgatg ccaaaaccgg tggtgagaag
     6421 accgccaagg aactgggggt caatttcatc tggaccggct cgaacacggc ggacgccgcc
     6481 aatgaagtaa ccatcatcga aggcctaatt caaaagaagg ttgacggtct gattatcagt
     6541 tgtaacgagg ccggagcctt aaaggacgta atcgaccggg cgacagccgc cggaatcaag
     6601 gtggccacct ttgattccga ctcgccgcag agcaagaggg ttttttattg cggtaccgat
     6661 aactacaaag ccggtaaggc atgtggcgcc gcgatggtga aactcattcg tgaacgtaaa
     6721 ctggccggaa agaccctgac ctgcgccctc ttaaccggcg gcttggctgc cccgaacctc
     6781 aacgaccgga tcaagggatt caaagaagtg gtggcagcgg agaagatcaa cctcaaatac
     6841 ctaaacaccc tggcttgcga cgatgacacc aatcgggccg tcgaactgat ggagcagtat
     6901 atcaaggcca atcccaaact ggatactttc tttgtcgtgg gcggctggcc gttcttctcc
     6961 gccccgggct cgatgcccaa tatcaaggca tgggtcaaga aaggcggcat cctggtttcg
     7021 atggacacct tctacccggt gatggtggcg atgaaggatg gtctggccaa tgccctggtg
     7081 ggccagaact ttaccgctat gggtgagctc tcggtccgca ccatggtgga tctgttgaac
     7141 gggaagaatg ttaccgaatt tatcgatacc ggcatcgtca acgtcgataa aagcaatttc
     7201 gataaggttt ttgccgagac gattccctgg aaataacatt aacccggttc attcagagcg
     7261 aaaaacgttt aatcgctcaa taccggattg cagtagcctg acataaaaaa accagccttt
     7321 ggctggtttt ttctttaaat tatgcggcag gccgcataac ctctttattt ccctgtttgc
     7381 ttgctctttt cccgtaattc cacccggcgg attttcccgt ttatggtttt gggcagctca
     7441 ctgacaaact cgatgatacg tgggtacttg taaggcgctg tagcctgctt aacataatct
     7501 ttgatctcct ccgccagttc gccggaggca gtatagttac gcgtgagtac gatggttgcc
     7561 ttaacgacct gcccgcgcac aggatccggc gcggcggtaa cggcacactc caatactgcg
     7621 ggatgctcta tgagtacgct ttcgatttca aaagggctga tccggtaacc ggaggatttg
     7681 atgacatcgt cggttctgcc gacgtaccag aggtaaccgt cctcgtcctg ccatgctgta
     7741 tctccggtat gataataacc gtcgtgccag acatcatcgg ttttttcctt gtcattatag
     7801 taaccactga aaagaccggc aggtttgccg tgcttggtac agacaacgat ctcgccgact
     7861 tctcccgttg gaacaggttg acagttttca tccacgatcg agatttcata ctggggagaa
     7921 ggctttccca tggagccggg cttcggttcc atacccttcc aatttaatac cgtcagtgtc
     7981 gtctcggttt gtccgaagcc ttccatgagt tttaatccgg tgtaatggtg aaatttattg
     8041 aacacctcgg gatttagcgc ctctccggca acggttgcgt attggagcga agaaaggtcg
     8101 aatccttcga tgccttcctt gataaagaaa cgatagatgg tgggcggcgc acaaaaggtg
     8161 gtgattttgt acttttctat ttttgccagc agatccgccg gtacgaactt gtcgaaatca
     8221 tagacaaaaa ccgccgcttc catcagccat tggccgtaca atttgcccca tacagccttg
     8281 ccccaacccg tatcggcaac ggtaaggtgc aatccatcag ggtctacatt ctgccagtgc
     8341 tttgcggtgg taatatgccc cagagggtac gcaaaattat ggcaggccat tttaggcagg
     8401 ccggaggtgc cggaggagaa atatatgagc atgggatcac ttactttttg cttttgggct
     8461 ccccgtggcc gctcaaagtt ttcgggcgca gcttccatat tctccgtaaa actatgccag
     8521 ccatcctttt ttcccctggc aataattttt atcgtaatcg aggggcactc ggcttgcgct
     8581 tcatccacat aatttgtgat ttcgcccatg gccgtacaga ccacggcttt cacgcctgcc
     8641 gtgttgaaac ggtacacaag gtccttcgtg gtaagaaggt gtgttgccgg gatggcgact
     8701 gcgccgagtt tatgtaatgc cacaatggaa aaccagaatt cataatgccg cttgaggatc
     8761 agcatgacca tatcgccttt tccaatccca aggcttttaa aaaaattagc agtttggtcg
     8821 ctgtatttct tgatctcggc aaaggtgaag atcctttcgg cgcctgtatc gtcacaccat
     8881 accagggctt ttttgttagg ctccaacctt gccatctcgt ccacaacatc gaagctaaag
     8941 ttgaaatcag ccggaacatt caagctgaag gcttttaaca ttcctttttt atcataggtt
     9001 tcatttacgt acttcaggtg aaggccctgc attgttatgg tctttccttt ctcgttgaat
     9061 tcaaagcggg ccaaagcgga attccatgct cgctttgagt ttgttttgaa tgatattctt
     9121 tcgattgcaa gtaattattg atttttttct ttgggtttcg gaattacaat ggccaggaat
     9181 tggcagtccg caccgttcat ggctgtcatg ccgtgcggat ggcccgaatc atataataca
     9241 ctgtcgccgg cgtttaaaat ctccacatga ccgtcaaggt ccactttgag gctgcctttg
     9301 aggatgtaat caaattcctg ccccgcatgg gtcgagtaat acaggggggt gttttgttcc
     9361 ttctggttgt acggaacggt cacaaggaac ggctcgacca tcttgtcttt aaggcgatac
     9421 gccagatgct tgtaatcaaa tccctttctg cgctcaatcg gaagcccccc gccttttcgc
     9481 acgactgtgt aaaacgaaag cgtgggatta ctcccggtga gcaattccac aatatctact
     9541 ttgaaccttt gcgcgcattt catgagaaat gtaaatgtaa aatccgaatt tccgttttcc
     9601 gccgcaagat agtcgtcaat cgtggttccg gtaacatccg ccatttcttc ggctgtgata
     9661 tttagaatct cccgcattcc tttgattcgt tcggaaattt ccaaaatatg cgtctccatg
     9721 tggcttcctc ctttacaacg gttgatcaat gttgattgca aaatattgtc tgccaaaaat
     9781 attgtctgcc ataataatta tatatttaat ttcatcatat tcaatagaga cataaaaatt
     9841 ttctttgaat aagttttata gttcttatag agagcactcg ggcgcaccta ttaaaaagtc
     9901 ctccatatcc attgatatgg aggactttcg cgcccttatt tcttgggttt ctgggtatcc
     9961 ggcttcttcg tagcaggagc cttcttctgt tcaaccatcg aagttcgcct cccttcgaca
    10021 agcttgtcag ctaatgtcaa aaagccccag aaaaaatata aagttcaaaa aagccttatg
    10081 gcttttctta acccctatta tagtacaaaa aacacatttg tgtgaggaaa tatgttaaat
    10141 ctaggttaat ttatacccgg ttcgtttttt gagacaccgt aatgttttcc aatgatatct
    10201 tgtcttcaat ggaaaatatc catttgttgg caggagatcc ctccatcatt gccgaagaat
    10261 aaataaaatt caactaaaac tgctggttct cataaagaac gatcatttcg gctaacactt
    10321 ccatgatatc actttcgtta atggataaat cttctgcttt aatagaagcg gatacaactt
    10381 tttgaatcag agttttggct aaagaccggc cagtcgttaa ttctttctgc cgtgaataaa
    10441 gattcttcat gattaagtag tccgcttggg tgatcgatgg cacgatcccg ggtaaccagg
    10501 gatatcgctc aatcacagct gtagattccc cgggtgaaac aaccgtctgg gctgcctgct
    10561 catctagaaa ctttttaact ttgcgacggg gtaattcttt gacgacaatg gtcccggcgg
    10621 catagtcacc gagccgtttg gccttttggt tgaaaaacat ggttatcatt ccaaacccat
    10681 aaaaaaaagg caaataatcg accatccgga tcacattccg tagcagaaca tcccaaaaag
    10741 aaggagcata accgccttct ttgcgaaccc ggatattgac caactttttg ccgacggttt
    10801 ggccgttcat gaccgtctcc aagatgataa aataaccgat caacaccaaa aacaacaagc
    10861 cgatcagcaa tgaaccggcg aatgaagtag accagtgggt aattttactc cgaagttcgc
    10921 ccaatttcag cataccgagg gaacttaaaa ttccgatgat caacacccac tgaatcaatg
    10981 tatcaatgac gattgccaaa aaacgcgaac cgatgccggc aacttcataa tccagttcca
    11041 cattttccgg agtaatcact ttataaacct cattcatcgc ggatacctct ctttgatctc
    11101 tatgataact atcaatcagg agtgaaatca tggcgattcc cttcgtacaa gcccggcagg
    11161 aacgttggat ccggctgcag aacttggtga aaaaggcgtc cggcaggaaa ttaacctctt
    11221 tttccaagga agagctgatt gaactgtcgg ttctttatcg gcaaaccgca acggatctgg
    11281 ctttggcaaa aactcaaggc ttgcccgatg agattaccag ttttctgaat gacttgacaa
    11341 ccaactccta tcattacatc tacaaaacag aaaaatcaac cctccgccaa ctcggtgact
    11401 ttatcgttca tgaatttcct gaacttttcc gcgaaaactg gcgggtgatc ctgtttgcct
    11461 tttcattact cttcgccggc tgggctgcag gattttgggg atatatagcc ggacccgact
    11521 tcatcgccgg gttgctgccg gaaaagttta ccaccggcat tttttcgggt taccaagaaa
    11581 aaacttggtt caatgaacca ttagttgcca gaccctatgt ctcctccgtc atcatgaata
    11641 ataacatcca ggttgccatt caagccttcg gcggcggtat gctgttaggt accctaactt
    11701 gttgggccat cttattcaat ggtttcatat taggagtttt agcagcggtt ttttttcaaa
    11761 aaggctactt actgtcgttt tgggcaatga tcttacccca cggggtgatt gaactgaccg
    11821 ccataggcct gtcagccgcg gcaggactgt tattgacccg tgccattctg ttcccgggcg
    11881 attacagccg ctccgatgcc ttaaaaacct gcggcatcaa agcagtcaaa ttattggcag
    11941 gcaccatttg catgctgatt gtagcgggat tgattgaagg ctttttcagc accattagca
    12001 ccgacctcat ccctgaatcc gtccggctgg tatttgccgc cgtaaccgcc attctgttat
    12061 ttctctattt taaaccgcgt cttttgttcc ataggaagac cgatagatcc gacggctcat
    12121 gaatgagcta aagagacgac tcacattacc atacaaaccc agtttaaaat aacccgcgtt
    12181 ttttcaggta gatgtaatgt ttggctacca cctgccgaat gttggcgggg ttgacttcca
    12241 ataccccaat cccgtaatcg gttaag
//
LOCUS       DASVMB010000440         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_644135,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000440 DASVMB010000000
VERSION     DASVMB010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644135"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF531_12365"
     CDS             complement(<1..228)
                     /locus_tag="VF531_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEX7714799.1"
                     /translation="MEERPYRYFDLILGVFVAILLISNLVSVKAVRIPVPFTGWHFSF
                     DGGTLLFPFSYIFADVLTEVYGYERSRRVIWS"
     gene            complement(453..2360)
                     /gene="dxs"
                     /locus_tag="VF531_12370"
     CDS             complement(453..2360)
                     /gene="dxs"
                     /locus_tag="VF531_12370"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7714800.1"
                     /translation="MSDYTLLETIKEPSDLKRLSNQELENLAGEIRRFLLETVAQTGG
                     HLAPNLGVVELTLALHTVFDSPRDQIVWDVGHQSYIHKLITGRFARFDTLRQYNGLSG
                     FPRPDESIHDAFGTGHSSTSISAALGLAKARDLLGETNKVVAVIGDGSLTGGMAFEAL
                     NHLGHLQTDMIVVLNDNEMSISKNVGALSRYLTRIRVNPHLRRLKYDIQDFIQKIPRI
                     GQATVNYLEKLEDSLTFLVIPGMFFEELGISYLGPIDGHNITDLKMIFNHARERKGPV
                     LIHVLTTKGKGYSPAEKYPQKFHSTGSFELKSGRKLEQSPAKSYTDIFGATIVDLAQA
                     DPKIIAVTAAMADGTGLSDFARRFPARFLDVGIAEEHAVTMAAGLAKKGFRPVVAVYS
                     TFLQRAYDQIIHDVCLQNLPVVFMLDRAGLVGPDGPTHHGIFDLSFLRQIPNITVMAP
                     KDEPEFRNMIYTALRMSGPVAVRYPKREVPGGGIDPETYQDLVPGKGEVLLSGENTAI
                     IAIGSMVQTALEAADHLRKLGLNCTVVNARFVKPLDEEMLLQIARTHKQIVTVEENMV
                     AGGFGSAVLEFYTQNRVKNLDLRILGIPDQFITHGSNEVLLHQCGLDPEGICRAVREL
                     NLPLVGVRARV"
     gene            complement(2357..2935)
                     /locus_tag="VF531_12375"
     CDS             complement(2357..2935)
                     /locus_tag="VF531_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714801.1"
                     /translation="MSSNSWRIAKIALFTFTFSLVISFLFQFSLALWFSLVVLFFVVV
                     IGIGFDLIGTAVTAASEPPFHAMGADRIAGSRQAIYLIRHADRVANICNDVVGDIAGT
                     IGGAIIASIGVKLSALLVFLPEKLVGAMAIALIAALTVGGKGLGKSYAIRNANRIVFT
                     VGKLLNLTRFPGITDPKKIKARSKQRKARKSG"
BASE COUNT          861 a          855 c          785 g          756 t
ORIGIN      
        1 ggaccagata accctgcgtg aacgttcgta tccgtatact tcggtcaaga catcggcgaa
       61 gatgtagctg aatggaaaaa gcagtgttcc gccgtcgaag gaaaaatgcc atccggtaaa
      121 cggaaccggg atccggaccg ccttcaccga tacgaggttg ctgatgagta agatggcaac
      181 gaatacgcca agaatcagat caaaataacg atatggcctt tcttccatga cgttctttca
      241 aagactcctt tctttattgt catcaaatcc tgacgaagcc acgccttaaa ccgacaagta
      301 ttattataaa aaagaaattc gcccctttag ggtagctgct tgggttcgaa aacctgaatt
      361 caccacgaaa gtcacgaaat acacgaaaaa atcttaccaa tcgttttcgt gcttttcgtg
      421 tgtttagtgg ttgaataatt cattgaagat ggctagaccc gggcccgcac tcccacaaga
      481 ggcaggttca attcacggac ggcacggcag ataccctccg ggtcaagacc gcactgatgg
      541 agtaacacct cgtttgatcc gtgagtgatg aattgatccg ggatgccgag tatccggaga
      601 tctaaattct taacccgatt ttgggtgtaa aactccagga ccgcggatcc aaaacctcct
      661 gcaaccatat tctcctccac agtcacgatc tgtttgtggg ttctggcaat ttgcagtaac
      721 atctcctcat cgaggggttt gacgaaccgg gcgttaacta ccgtgcagtt taggcccagt
      781 tttcgtaagt gatccgcagc ttccagggca gtttgaacca tagaaccaat ggcgatgatt
      841 gcggtgtttt cccctgacag caggacctcg cccttgcccg ggaccaaatc ctggtatgtt
      901 tcaggatcga tcccgccgcc gggaacctca cgtttgggat aacgaaccgc cactggaccg
      961 ctcatccgga gggctgtata gatcatgtta cgaaactccg gttcatcttt tggcgccatc
     1021 acagtaatat tggggatctg ccgcaaaaag gagagatcaa agatcccgtg gtgggtcggc
     1081 ccatccggcc ccacgagtcc ggcccggtcg agcatgaaaa ctaccggaag attttgaaga
     1141 caaacatcgt gaatgatctg gtcgtaagcc cgttgcagga aagtggaata aaccgccact
     1201 accgggcgaa agcccttttt cgccagaccg gcagccatcg ttaccgcgtg ttcctcggca
     1261 atccccacat ccaaaaaacg ggccgggaat cttctggcaa aatcggagag gccggtaccg
     1321 tcggccatag ccgccgtaac ggcgataatt ttcggatccg cctgcgccaa atcgacaatg
     1381 gtcgctccaa agatatcggt ataactctta gccggagact gctccagctt ccgtccgctt
     1441 ttcaactcaa atgacccggt actatgaaac ttctgggggt atttttctgc gggtgaatac
     1501 cctttcccct tggtggtcag tacgtgaatc aggaccggcc ccttacgttc ccgggcgtgg
     1561 ttgaagatca tcttgagatc ggtgatgtta tgaccgtcaa tcggacctaa ataactgata
     1621 cccagctcct caaagaacat gcccggaatc accagaaaag tcaaactatc ctcgagtttt
     1681 tccaggtagt tgactgtggc ttgtccgatc cgcggaattt tctggataaa atcctggatg
     1741 tcgtatttca gccgccgtaa atgggggttg accctgatcc gggtaagata acgcgaaagc
     1801 gcgccgacat ttttggagat cgacatctcg ttatcgttga ggaccacaat catgtcggtc
     1861 tgaagatgtc ccaaatggtt cagggcttcg aaggccatac ctccggtaag cgaaccgtcg
     1921 ccgatgacgg ccacaacttt attggtctct cccaacaagt cacgggcttt ggcaagccct
     1981 aatgcggccg agatcgatgt ggaactgtgt ccggtaccga aagcatcgtg gatactctca
     2041 tcgggccggg ggaaaccgct taacccgtta tactgcctca aagtgtcaaa ccgggcgaac
     2101 cgaccggtga tcaatttgtg aatataggat tgatggccga catcccaaac gatttgatcc
     2161 cgcgggctgt cgaaaactgt atgtaaggcg agagttaatt caactacgcc gaggtttggg
     2221 gccaaatggc caccggtctg ggccactgtt tccagcaaaa agcggcgaat ttccccggcc
     2281 agattctcca gttcctgatt ggacaaacgc ttcaaatcgg atggttcctt aatcgtctcc
     2341 aagagcgtat aatcgctcat ccactcttcc tagcctttcg ttgtttagat cgggccttaa
     2401 tttttttggg gtcggttatt ccaggaaatc tcgtaagatt gagtaattta ccgactgtga
     2461 aaacaatccg gttcgcattg cggatggcgt aggacttacc caagcccttg ccgcccacgg
     2521 ttaaggcggc aatcagggca attgccatcg cacctaccag tttctccggt aggaatacaa
     2581 gtaatgcgga gagttttaca ccaatactgg cgatgatggc accgccaatg gttccggcga
     2641 tatcaccgac cacatcgtta cagatgttgg caacccgatc ggcatgacgg atcaaataaa
     2701 tcgcctgccg tgagccggcg atccggtcgg cccccatagc atgaaaaggc ggctcgctcg
     2761 ctgcggtgac cgccgtacca atcaaatcga agccgatccc tatcactaca acaaagaata
     2821 acaccacaag agaaaaccat aacgccaatg aaaactggaa caaaaaggat ataactaatg
     2881 aaaacgtaaa agtaaataag gcgatcttgg cgatccgcca ggaattggaa ctcaagaaaa
     2941 cctccaaaca caaaatatgt agggatggta tccatcgagt aaaaattatg gcttaggtcc
     3001 agtaggtttt cccaaagacc tccgctccgt caccggaacg gtggtttccc tttaaggtct
     3061 ctccgccttg tcgccaaaag cgagactgga ttaccactta gtccacactg caatccccga
     3121 tggaactcaa aatattgaat agcctaggag ttttccccca caaaacgccc cactttctag
     3181 cctcttttgc gataaagggt ttcatatggc aaagagccac acgaccctgc agaagtagag
     3241 tgacttgata cccatat
//
LOCUS       DASVMB010000441         4446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_423371,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000441 DASVMB010000000
VERSION     DASVMB010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423371"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VF531_12380"
     CDS             complement(<1..1065)
                     /locus_tag="VF531_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7714802.1"
                     /translation="MRKVWLNAYEKGIPGQLEYPDTTLPAALAQIASEFPNNHALVYL
                     DYSWSYSELDKLVSQMANLLIGMGIKPGERIAIHLPNSPQFVIAYYGLLRAGAIAVPI
                     NPSSTGDDFEFILENSGSKILVTFSDILPNLKLDAAIGIKILVTHIQTPFAPNQDYQI
                     SPVPSVTRLERVLFDQSTINPGIMISPDAYANLQYTGGTTGRSKGAILSHRNLIANAC
                     QFKHWFKNSYNQGEGRFIGVIPLFHIYAMTTTMNAPIITASTILLQSKFDISELMHII
                     ERYKPNLFMGVPAMYGAITLRQQHPDMSSIKACMSGSAPLPPAIQKNFEYLTGGKLAE
                     GYGLSEASPIVCCNPIYGNVK"
     gene            complement(1424..1696)
                     /locus_tag="VF531_12385"
     CDS             complement(1424..1696)
                     /locus_tag="VF531_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HEX7714803.1"
                     /translation="MIYRNGKEILPAELLREIQQYVQGELIYIPKPETSRAGWGERNG
                     TREILRSRNTEICSLYWSGSPVAELIDRFHLSEDSVKKIISHSRRQ"
     gene            complement(2114..2461)
                     /locus_tag="VF531_12390"
     CDS             complement(2114..2461)
                     /locus_tag="VF531_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7714804.1"
                     /translation="MKTLPCKGCHGLCCGPVPVTRQELWNITREIRSMPPKVRFELEN
                     QPRHFGTCIFYDLAQDKCGIHQVKPSICRAFGHYKNLACFRQPGAAANEDWNVLELPA
                     GLLTVDFTWKDFN"
     gene            complement(2734..3414)
                     /locus_tag="VF531_12395"
     CDS             complement(2734..3414)
                     /locus_tag="VF531_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HEX7714805.1"
                     /translation="MKFQDSLQLLRKQKGISQEKLAERIGISRQAVAKWEVGQSYPDM
                     KNLIMLSDLFGISIDKLVKDNDDQSCLYEDGKQQPYIDEKVIEFLCNAKKATYAGKGR
                     ETAPSRPRSHDLQYTENNLKYIDTYLGGEKFAGEEALWCDDLPFWSMNYVGRIIADGF
                     SGDFLKECLLLVQKEYPYRGPLVHQNGEYTYHCVVNGEFEWFTGYEEIFRNDLKVYEC
                     RFHGGCIK"
     gene            complement(3956..>4446)
                     /locus_tag="VF531_12400"
     CDS             complement(3956..>4446)
                     /locus_tag="VF531_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714806.1"
                     /translation="RSGPKKCLQDDFITHSEDTLFRGTFCGAIAGILKDSIDYIFYAV
                     NTEGVFFWSFASVIAFGKLPKGLFPDIFGLTLEIIFSAFLGFIYAGVTKKIKTRHYLI
                     NGIFFGSMVWFLIRAAIVGYKINELKTVGIPVRPFITWFLSLVYGITLAWLERKLSPK
                     TS"
BASE COUNT         1193 a          956 c         1002 g         1295 t
ORIGIN      
        1 tttaacatta ccgtaaatcg ggttacagca gacgatgggg gaagcctcgg aaaggccgta
       61 tccttcggca agtttaccac cggtgaggta ctcgaaattt ttttgaatcg ccggcggcaa
      121 gggggccgag ccgctcatac aggctttgat ggaactcata tccggatgtt gctgtcgcag
      181 ggtaatcgcc ccgtacatgg ccggtactcc catgaacaaa ttaggcttgt acctttcgat
      241 gatatgcatc agttccgaga tgtcaaattt ggactgtaac aagattgttg aggcagtaat
      301 tatcggggca ttcatcgtgg tagtcatggc gtaaatatgg aaaagcggaa ttacgccgat
      361 aaaccgtcct tcgccttgat tatagctatt tttaaaccaa tgcttgaatt ggcaggcatt
      421 ggcaatgagg ttgcgatggc tcaaaatggc ccctttggag cgcccggtgg tccctccggt
      481 atattgcagg ttggcgtatg cgtcggggct gatcataatc ccgggattga tagtggattg
      541 atcaaacaaa acccgttcta atctggtaac tgaaggaact ggactgattt gataatcctg
      601 gttgggagca aaaggagttt ggatatgggt aaccaaaatt tttatgccta tcgcggcgtc
      661 taacttcaga ttcggcagga tgtcactgaa agtgacaagt attttggaac cgctgttttc
      721 gaggataaat tcaaaatcat ccccggtgga actggggtta atcggaacgg caatagcgcc
      781 ggcgcgtaat aagccgtaat aagcgatgac aaactggggt gaattgggta ggtgaatcgc
      841 aattctttca ccaggcttaa tacccatacc gattaataag ttggccatct gggataccag
      901 tttatcaagc tcactataag accaggaata gtcgaggtaa accaaggcgt gattgtttgg
      961 gaattcgcta gcgatctgcg ctaatgctgc cggcaaggtt gtgtcgggat actcgagctg
     1021 ccccgggatt cccttttcat acgcgtttag ccatactttc cgcaaataac tcatcctttc
     1081 cggtgcggga ttgattaagg cggcaattta aaaaagagca agatttgtaa atattcgaca
     1141 cattttttaa ataacctgct atgatccttt actttacaag atggggtggt ttgttggatt
     1201 cgcgtataat cagggttggc gagatgatat tgtactggat ttggaggtta gggtttttca
     1261 ttttatttag taaagcttcg gcggcatgga cgccgatctc gaatacgttt ggcggagata
     1321 tcatcattgg ccggtccgcc aatgaaggcg attcgttttg tcgtaacata taaaaatagg
     1381 gcacttggcc ctattttagg tacgcccaca gcatgggcgc aatctactgc cttctggagt
     1441 gactgatgat cttcttcacg ctgtcttccg agagatgaaa gcggtcgatc agctcagcca
     1501 caggtgaacc gctccaatat aaactgcata tctcagtatt gcggctgcgc aggatctccc
     1561 gggtcccgtt ccgttcaccc cagcctgccc gggaggtctc cggcttggga atgtagatca
     1621 attctccctg aacgtattgt tggatctccc gcaagagttc agccgggaga atctcctttc
     1681 catttctgta aatcaccaat gaaagcctcc tctaaacgtg aagagttgac gaatgccagt
     1741 tcagctttca tggtttcacc tcctttgaaa agattgtttt ttgagaaacg caccgatctt
     1801 aagtaggggt ggggagaatt ccccatccct gacctttggg tgcagcgtcg tcaggcgtaa
     1861 aacgtcttta gacgtcaaac gtccttagac gttccaccac ctatcatcgt atttcacctc
     1921 aaccatctcc tttcaaagaa gattgatctc gcgcgatcga ttctattatg cgacatctcc
     1981 tgaaattcgg caagggaaaa atcggctata gttaattcat ctttttcatg actcactggg
     2041 ttagttgccg gatttttcat ggagaaatac ccctttaggg cggagggcaa tgatttctca
     2101 agaagcattc agcctaattg aaatccttcc aggtgaaatc aaccgtgagt aagccggctg
     2161 gaagctccag gacattccag tcttcgttgg cggcagctcc gggttggcga aaacaagcca
     2221 agttcttata atggccgaaa gcgcggcata ttgagggttt tacttgatgg atgccgcatt
     2281 tgtcctgagc cagatcgtag aagatgcagg ttccgaaatg gcgcggttgg ttttccagct
     2341 caaaacggac ttttggaggc atggatcgga tctctcttgt gatattccac agttcttgcc
     2401 gggtgactgg aaccgggccg cagcataatc catgacatcc tttgcacggc agagttttca
     2461 taagaccccc gaattattct tcaaaggttg cttcgtattt ccgttaatca acgaacgatc
     2521 actcaataac ctgatcctat catacggtca ggtaaatttc aataataaat tatttattat
     2581 atcataaatt gttaattttg tcaagtgatg tttttgccgt atcatcctgt aataagatga
     2641 aatggtatat ttactcatgc atcccaagga atctttaata tttcgtgatg tcacgcagca
     2701 tttcatatta aaaaaagggt tatcttttca tgattattta atacagccgc catggaaacg
     2761 gcattcgtac accttcagat cgttgcggaa tatttcttca tagccggtga accactcaaa
     2821 ttcaccattt acgacacaat ggtacgtata ttccccgttt tgatgcacca atggtccgcg
     2881 atacggatat tctttttgta ccaagagcaa gcattccttc aaaaaatctc cggaaaaacc
     2941 atcggcgata atccggccaa cgtaattcat ggaccaaaac gggagatcat cacaccataa
     3001 tgcctcttct cctgcgaatt tttctccgcc caagtaggta tcaatgtact ttagattgtt
     3061 ctccgtatat tgtaagtcat gagaacgtgg cctggaagga gcggtttccc ttcctttgcc
     3121 ggcgtaagtg gctttcttgg cattacataa gaattcgatc actttttcat cgatatacgg
     3181 ctgttgtttg ccatcttcgt ataagcaact ttgatcatca ttatctttta caagcttatc
     3241 aatactgatt ccaaataaat cactcaacat gatcaagttt ttcatgtcgg ggtaagactg
     3301 cccaacttcc cactttgcca ctgcctgtct ggatattccg attctctcgg ctaatttttc
     3361 ctgagagatt cccttttgtt ttcttaacaa ctggagtgaa tcttgaaatt tcatcttcat
     3421 cacctccatc taagaatata aagatagcga tgggatttct accaatccgg gataacaaaa
     3481 atgacaacca ttggttgcct gggataatcg aaacgtatag aagtaccttt tgaatacctt
     3541 aatcccatcc gctttggcgt tgccgccttc gatcttgaac accttctgca gcagtttaca
     3601 tgatttttga ttgggacctt catggcgatg attgctccat tgattggcgt aattgcggtc
     3661 ttcatagtgt ggggcattca tgaaaagtgt ctaaattcat tataaacgat acagatttat
     3721 aaaagcaaat tccttacaac cgatgaaagc atggcagtag tcaattttgc aaaatatcaa
     3781 ggttggatag ttgatagagg cagggtaata tgcacatctc tgttggggat aaatgtggtt
     3841 cgtcattttg taatttcgcg gtttggggcc tctctttttt ggcatagtgg agttttactg
     3901 ctcaattttt aggggaatag aaagcagata tgaaaaaaga aaatgttgtt aaaggttaag
     3961 aggttttcgg agaaagcttt ctttctaacc atgcgagcgt aattccatat accagcgaca
     4021 agaaccaggt gatgaagggg cgaaccggta tacctacagt ttttagctcg ttgattttat
     4081 aaccgacgat ggctgccctg atcaaaaacc aaaccatgct gccaaagaaa atgccgttga
     4141 tgagataatg ccgggttttg atttttttcg taaccccggc atatatgaat cctaaaaagg
     4201 cgctaaagat gatctccaga gttaacccaa atatatccgg aaacaaccct ttgggtagtt
     4261 taccgaaagc aataacggaa gcaaaagacc aaaagaatac tccttcagtg ttcactgcgt
     4321 aaaagatata atctatacta tctttgagaa ttcccgcgat agccccgcag aacgtacccc
     4381 taaataaggt atcttcagag tgcgttatga aatcgtcttg gagacatttc ttaggtccgc
     4441 ttcttg
//
LOCUS       DASVMB010000442         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_568661,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000442 DASVMB010000000
VERSION     DASVMB010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568661"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2123)
                     /locus_tag="VF531_12405"
     CDS             complement(87..2123)
                     /locus_tag="VF531_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEX7714807.1"
                     /translation="MPSQLRPPAVPLITVDPYFSVWSFANRLYDDFTRHWTGAKNSLT
                     GFVWIDDQPYRFAGRMDPSQDPEDQEPPEMLQTGLSIKPLSSIYMFEAGGITLQVDFT
                     TPLLPGNLDILSRPVSYVSFTAHSNDRRPHHLKLYFDVSAEWCVNNPNQKVVGGRKKL
                     GAGISTMYIGTEEQAVLSRSGDDLRIDWGFINLATNDCFQTETVIAANRIRQDFIRHG
                     ELHTKDLAAGPSVIGSDNPVMACAIDLDEVNGMPVSTFVTLAYDDIDSIGYFGKHLPA
                     YWRRNGLSFSEMLESAISEYPEIMRQCAEFNQRLKNDAIQAGGSKYADILSLAYRQAI
                     AAHKLVADSEGQVLFISKECFSNGCMATVDVSFPSIPLFLLYNSELVKGMMRPIFKYA
                     ASPEWTFDFAPHDIGQYPLANGQVYGKEKNEILLRYQMPIEECGNLLIMMSAVCSMEG
                     SAAFAGENWDLLLKWAHYLKEHGLDPENQLCTDDFAGHLAHNANLSIKAIVALAGFAK
                     LCRMLGKNETAAEFLKTAQEMAGQWEKKAQEGDHYKLTFDQPHTWSLKYNLLWDELLG
                     LGIFAPEIHRKELDYYLQKQNRYGTPLDNRSTYTKADWLVWAAALADTKETFERLVEP
                     LWLFLDETPSRVPFTDWYDTVSGRQIGFQHRSVVGGLFAKLLKDRIKGRSIHSF"
     gene            2423..3010
                     /locus_tag="VF531_12410"
     CDS             2423..3010
                     /locus_tag="VF531_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7714808.1"
                     /translation="MESSWGNAAGCGGTYGYVVSQTELNLRESMRLLWEQHVYWTRMV
                     IISIAAGLPDLDPTTQRLLRSDTDFEAAFTPFYGAEIGHKFGELINAHLVIAAELVKA
                     AKAGDSQGASDAETRWYQNADQIVCFLNQINPYWDIEQMRALWYEHLSLTKSEAVLRL
                     GGNFAEDIATFDRIELQALMMADIFAEGIIRQFCL"
BASE COUNT          926 a          769 c          831 g          898 t
ORIGIN      
        1 gataaacctg agagccacgg gtggcgacat aaggaaaacg gagcccgatg ggctccgtca
       61 atatattgcc gtcagttatc tataactcaa aaggaatgaa ttgatctccc cttgatccga
      121 tcttttaata gtttggcgaa aagcccgcct accaccgagc ggtgttgaaa tccgatctgg
      181 cgccccgaaa ccgtatcgta ccagtctgtg aacggtaccc ggctgggcgt ctcatctaag
      241 aaaagccaca gcggttcaac cagtcgctca aaagtttcct tggtgtcggc cagagcggca
      301 gcccaaacca gccagtcggc tttcgtgtat gtgctgcggt tatctaaggg cgttccatac
      361 cgattctgtt tttggagata ataatcaagt tccttccggt gaatctccgg cgcaaaaata
      421 cccagaccca ggagttcatc ccagagcaga ttgtatttaa ggctccaggt atgtggctga
      481 tcgaatgtta atttgtaatg atccccctct tgggctttct tctcccattg cccggccatt
      541 tcctgggcgg ttttcaggaa ctcggccgcg gtttcgttct ttccaagcat tcggcaaagt
      601 ttggcgaaac cagccaaggc gacaatcgct ttgatggaaa gattggcgtt atgggctaga
      661 tggccggcaa agtcatcggt acagagctgg ttctcgggat caagaccgtg ttctttcaga
      721 tagtgggccc actttaagag cagatcccag ttttctccgg cgaacgcagc cgagccctcc
      781 atcgaacaaa cggcactcat catgatcaga aggttgccgc attcttcaat cggcatctga
      841 taacgaagta atatttcatt tttttccttc ccgtacactt ggccgttggc caaagggtat
      901 tggccgatat catgaggtgc aaaatcaaat gtccattccg gggaagccgc atatttgaaa
      961 atcggccgca tcatcccttt gaccagttca gagttataaa gcaaaaacaa gggaatggag
     1021 gggaagctga cgtccaccgt agccatacac ccattgctga aacattcttt ggagataaac
     1081 agtacctggc cttcgctgtc cgccaccagc ttatgggcgg caatggcctg gcggtaagct
     1141 aacgatagga tatccgcata tttggaacct ccggcctgaa tcgcatcatt tttgagccgt
     1201 tgattgaatt ccgcacactg cctcatgatc tccgggtatt cggaaatggc cgattccagc
     1261 atctccgaaa agcttaaacc attacgcctc caataggccg gcaaatgctt cccaaaatac
     1321 ccgatggaat caatatcatc gtacgctaag gttacaaagg tggaaaccgg catgccgttg
     1381 acttcatcaa gatctatggc gcaggccatc accggattat cactgccaat cacagatggc
     1441 ccggccgcta aatctttggt atgcaactca ccatgccgga taaaatcttg gcgaatccgg
     1501 ttcgcggcga tgacggtttc ggtttgaaag caatcgttag ttgccagatt gataaaaccc
     1561 cagtcgatcc ggagatcatc accggaacgg ctcaataccg cctgttcctc tgtgccgata
     1621 tacatggttg aaataccggc ccctagtttt tttcggcccc caaccacctt ttggttgggg
     1681 ttatttacac accattcggc actgacatcg aaatacagtt tcaaatgatg aggccgccga
     1741 tcgttagaat gtgcggtaaa gcttacataa gacaccggtc tggacaagat atccagattc
     1801 cccggcaaca gcggggtggt aaagtcgact tggagggtta tgccacccgc ctcaaacata
     1861 taaatacttg aaagcggttt aatggagagc cccgtttgca gcatttccgg gggttcctga
     1921 tcttccggat cttgggatgg atccatcctg ccggcaaagc gatagggttg gtcatcaatc
     1981 caaacgaagc cggtcaaaga atttttggct ccggtccaat gccgggtaaa gtcatcataa
     2041 agacggttcg caaaggacca aacgctaaaa tagggatcga cagtaatcag tggtacagcc
     2101 gggggccgta attgtgatgg catcaatatg ctccttttca tttttattaa ctatttagtt
     2161 aatcaattca cttatttatt aacaaattga ttataattcg ctatgaactt cttgtcaaca
     2221 ttagatctat agagtcgaca acaattgagt cgattatatt gattaactct aacgttacgc
     2281 aattggaatc aaaaacaact gaagaggcat atgatttttg tactactccc tccaaattgg
     2341 gagcacccct gtttcgacat ggtccgataa cgatgttcta catataatgt taaaaagtcc
     2401 aagatcaggg ggcgatgcga tagtggaaag ttcttggggg aatgctgcgg gttgcggtgg
     2461 cacctatggt tatgtagtca gtcaaacaga gctaaatcta agggaatcca tgcgtcttct
     2521 atgggaacag catgtttatt ggacccgaat ggttatcatc agcattgcgg cgggcttgcc
     2581 cgatttggat cccaccactc agcggttgct tcgcagtgat accgattttg aagcggcgtt
     2641 tacgcccttc tatggcgcgg agattggcca taagttcggc gaattaatca atgctcattt
     2701 ggtgatcgcg gccgaactgg tcaaggcggc caaagccggt gatagccaag gggcaagcga
     2761 cgccgagacc cgatggtatc aaaatgcgga tcagatcgtc tgctttttaa atcagatcaa
     2821 tccttattgg gatattgaac agatgcgggc tttatggtat gagcatttat cattgaccaa
     2881 atccgaagcg gtattgcgat tgggaggtaa ctttgccgag gatatcgcta cctttgaccg
     2941 gattgaactt caggcgttaa tgatggccga tatttttgcg gaagggatta tacgtcagtt
     3001 ttgtctttaa agcgagttcc ggatggaata acttcaagcc gggggagtga atcctccggt
     3061 tattttttag cgaatgattg cccaatttaa ggatatagaa gtttaataaa ccatagttgg
     3121 gaaaaatgta ttgacaaatc ccggatgcaa gtttattata gtaataacct agtaattcga
     3181 taggaattat agaaattaga tcaactttaa ccggaacatt cgggcgttcc ggtttatcaa
     3241 aagacttaat tcttagtaat cagataataa ttaaagggat tactggaggg ggggttgaga
     3301 aaaaaactat cgatcaattc ctatgtatgg acacggtggc aacaattgga attccaaggg
     3361 agtttgttaa ctgtaaaggg aagtaaaata aactgaggag gttgtattga tgagtaaaag
     3421 gatc
//
LOCUS       DASVMB010000443        12290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_53442,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000443 DASVMB010000000
VERSION     DASVMB010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53442"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VF531_12415"
     CDS             complement(<1..1255)
                     /locus_tag="VF531_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX7714809.1"
                     /translation="MSEFTLAELETWNQRIEDCVQKAGLDYYPQEFEICSYEEMLGYE
                     AYTGMPSHYPHWSYGKAFDRQHTFYRYNLTGLPYEMVINSNPCLAYLMQDNTLLLQIL
                     TIAHVYGHNDFFKNNRLFKMTRPELTIETFKNHADRVRGYIQDPSIGYFKVERILDAA
                     HALRYQTNRTPGRRPETPAEQKERLLQAYYRRIRQDQGNKDSSETPPDLNKIPLVPEE
                     DLLWFIAEYGRLEEWERDLLRIVRTETEYFLPQMETKIMNEGWASFWHYRILNQLELS
                     ADFHFEFLKRHHQVIRPIPGGLNPYHLGFSIFTDLERRFGIAKIFEAREIESDRSFIR
                     RYLTRELAQELHLFEYLAEGDRAVVTEVADEEGWEKIRETLCLNLGAGMIPTIRVREL
                     IAKDRTLILEHEYDGRDLELTYAGET"
     gene            complement(1273..2529)
                     /gene="yhbH"
                     /locus_tag="VF531_12420"
     CDS             complement(1273..2529)
                     /gene="yhbH"
                     /locus_tag="VF531_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEX7714810.1"
                     /translation="MAIFRETAGDSGRAAEDRRRHRQLVEESIKKNIGQIVAEESIIG
                     QSGTKKVKIPIKGLKEYQFIYGRNRPGVGTGDGDEARGDVLQNGGKQGAGKGGQGAGN
                     EAGEDIYETEITMEELINYLFDDLNLPFMERKQLAAIETVTRKKRSGYQHKGTPPRLA
                     KKRAVIEKIKRRQGTRREPPDTGAGSPEPAPVDTTQEDVTGAMDSVGAGLPSGTGVES
                     SEASGRFPFREDDLRYHRVREEYAPDSNAVVICIMDTSGSMDQTKKYLARSFYFLLYQ
                     FIRMKYDRVELVFIAHTTQAVEVNEAEFFHKGESGGTYISSGYEKALQIIEERYPPIH
                     WNIYAFHCSDGDNWDEDNEKALALARRLCEVCNLFGYGEIMTGSSTIRNLYRQGIDAK
                     NLALVSIAGREDIWPALRDMLAREQD"
     gene            complement(2547..4475)
                     /locus_tag="VF531_12425"
     CDS             complement(2547..4475)
                     /locus_tag="VF531_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX7714811.1"
                     /translation="MESQIADLIKKDRSERKHQHFEGTFLDYLDIIRANPGIVQLAHQ
                     RMYHLITDPGVNVIKTEENPRLRRIYGNDILKQYPFFTGDFFGIDRTIMKLVRYFHAA
                     AMQGEESRQVLYLVGPVGAGKSSLMEHLKRALESAPPIYAIKGCPMREEPLHLIPKHL
                     RAKFEELLKVHIEGDLCPSCRYLLKNEYQGEYERFPIETVEFSIRSRKGVGVVPPVDP
                     NNQDTSVLIGSVDISKMDLYPEDDPRVLALNGAFNVGNRGVVEFIEVFKNEIEYLHTM
                     ITATQEKSIPSPGKGAMIYFDGVILAHSNEAEWNKFKSDHTNEAILDRIVKIEVPYCL
                     ELNEEIKIYQKLLKHSNFSAHIAPHTIEMASMFAILTRLSPSSKVDPLTKLKIYNGEE
                     ILEQGNTKRVDINELREEATNREGMTGISTRFIMKALDMTLTESECGCINPLAVMETL
                     VKSVKDLSLPDDERKRYLGFLQDTIRKEYHKLLELEVTKAFIHGYREQAQDLFNNYLD
                     HAEAYANRTKIKDRNTGEELEPDEKFLQSIEEQIGISGSAARGFRQDVTAYMFFVLRN
                     GGSLDYQSYEPLKVAIEKKLTASVREISRVITQAKVRDKEQNEKYNAMVAEMKENGYC
                     DHCCNVVLKYAANNLWKD"
     gene            4804..7572
                     /locus_tag="VF531_12430"
     CDS             4804..7572
                     /locus_tag="VF531_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7714812.1"
                     /translation="MEFERASSGLTGLDRVLDDLRLGDNVVWQVDDIADYRHFARAFA
                     AAALKSHKKLVYFRFGRHSPLIDPAPGVRLEPVNAARGFEAFSAAIRSVITAEGEGVY
                     YVFDCLSELLSEWSTDLMIGNFFLVTCPYLFHLKTIAYFAVLRDHHSYQTIARIRDTT
                     QILLDLYHDDRFYIHPLKVWKRYSPTMFLPHVAPDGSDVFVPLTTSADAVQLFASRKP
                     RGGLSHPARRLDYWDHLFIQAAETIQDSAGDSRTLAKRRQYLDKLLKMMISRDEMILT
                     LARRFLNINDLLKIKDRLIGSGFIGGKAVGLILARAILAASTKRDWNMLLEPHDSFYI
                     GSDVYYTYLVENSCWQLRQEQKLPENYYNTATILQEKLETGQFPEIIRDQFLEILEYF
                     GQSPIIVRSSSLLEDSFGNAFAGKYASVFCTNQGNLTERYDRFIQAVRQVYASTMNRD
                     ALAYRAQRGLANCDEQMALLVQRVSGGHHGEYFFPDLAGVALSHNLYVWREEMQPEAG
                     MLRLVLGLGTRAVDRVDDDYARFVALDHPLLRTETSSEEIRTFSQHGMDLLNLDLNQP
                     ETISIQDLINRNLIGQLPLFATPDISDDCFPDLGFPSRPWVFNFDPLLGETPFPRVMQ
                     ELLQTLQEAYGHPVDTEFTVNFRSSRDFSINPEENPSNGISGEKNENPASPSIRLPQE
                     APPERMQTNFYINLLQCRPLQVRCNQQPVALPDYIPGERVILESHGHFMGGNQELQIE
                     RFIYVVPQMYTGLSISDQYQVARVIGKLNRMIPPQDESPTVLLGPGRWGTSTPSLGIP
                     VSFAEINHFAVLGELAFQTGISTGGRQAGMIPELSYGTHFFQDLVETGIFYVALNEAV
                     EGTRVNHEFFMNHPSQLLRWLADAERWRSIIRVVEFSAAKTGLGRNEILRLHADVSRN
                     KMICWVES"
     gene            complement(7687..8865)
                     /locus_tag="VF531_12435"
     CDS             complement(7687..8865)
                     /locus_tag="VF531_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7714813.1"
                     /translation="MSSPEWKNIFTRRMPIAPLGIIPLASSRELAGLIDQQLLAQRRS
                     FLEHHPDYVAEPGFMPGSFLIPCDCIRFADGSGKAVINQTVRGSDIYIIADVGNYNCR
                     FKMFGLECPMSPDDHFQDIKRVIAAIGGRARRITVIMPLLYGGRQHRRQSRESLDCAV
                     ALQDLERLKVDNIFTFDAHDSRVQNAIPLTGFNNLLPSYQILKTLLEKEQDVVIDKSQ
                     MLIISPDEGGMSRSIFYANVLGLNVGLFYKRRDYTRVVNGKNPIIQHEFLGEEVEGKD
                     VLIVDDMIASGESILDIAKELKRRKARRIFFAVTFALFTEGLQEFREGYQQGLLNRVY
                     ATNLTFQKEDLKDEPWFVNVDMSQFIAYLLELLNHDQSISSLFDPSKKISEFIQKFQR
                     "
     gene            complement(9167..10822)
                     /locus_tag="VF531_12440"
     CDS             complement(9167..10822)
                     /locus_tag="VF531_12440"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7714814.1"
                     /translation="MKVTIAQLNPVIGDLAGNTQKIIEVWDRANREDSDLVVFPEMFL
                     VGYPPQDLLERDGFIRQVGDSLKRLAQISARYPKTGILVGAPTPTGGHVGKGLYNSAI
                     LIYRGQIIHNQPKSLLPTYDVFDEARHFDPAPQIVTVPFKAERLGISVCEDAWNDPDF
                     WPSRMMYDFDPITELAAQDATLLLNISASPFHIGKDELRYNLIRNHARKHGIPFIFVN
                     QIGGNDELIFDGGSMCFDKNGNPLTMGPFFEEWIQTVDMDRAGVARFYQPQEKIESVY
                     KALVLGIRDYLYKCGFKRAVIGLSGGIDSALTCCLATTALGKENVLGIAMPSPFSSAG
                     SIADSQRLAENLGIELLVIPITNIYHSYLDTLENHFRGKEHDTTEENIQARIRGNILM
                     AFSNKFGYLALSTGNKSELSVGYCTLYGDMSGGLSVISDVPKTMVYQLARFINRDQEI
                     IPETVIAKPPSAELKPDQRDEDTLPPYEILDQILHYYIEEGYSAEEIIARDFAPEVVQ
                     WVIRAVNRNEYKRKQAAPGLKVTTKAFGGGRRIPVAKKVDLGF"
     gene            complement(11048..12058)
                     /gene="amrS"
                     /locus_tag="VF531_12445"
     CDS             complement(11048..12058)
                     /gene="amrS"
                     /locus_tag="VF531_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX7714815.1"
                     /translation="MSNQETKVHEARFYEPVSDTLIGCGLCPQECRIGSGGTGLCKVR
                     INRDQKLTTTNFGQVSSLALDPIEKKPLYHYFPGSAIFSAGTIGCNLACEFCQNWEIS
                     KGDGAPTRLVSPAELAELAKSAEKQAGSIGVAYTYSEPGVWFEFLIETMPLLHEQGLK
                     NVLVTNGFLNPGPWQELLKWTDAANIDLKGFTEEYYRKLCHGRLEPVLENIKSAAGRI
                     HLELTTLIIPGENDAPDQLRELARWIGSIDPEIPLHLSRYFPNYKLTTAATPLETLAR
                     ARDIAREYLSYVYLGNAPGGSDTYCPECGNLLIERHGYETRVLTGESCARCGRATGIV
                     FH"
     gene            complement(12080..>12290)
                     /locus_tag="VF531_12450"
     CDS             complement(12080..>12290)
                     /locus_tag="VF531_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714816.1"
                     /translation="GETFASEVGWFWIRLDYDGIFADDSSCTGWFTTLTWKPKPFQWV
                     LRAAPWGDLNRRVGFYQVQVEYILP"
BASE COUNT         2894 a         3286 c         2998 g         3112 t
ORIGIN      
        1 gggtctcccc ggcatacgtc agctccaggt ccctgccgtc atactcatgc tccaaaatca
       61 aggtccgatc cttggcaatg agttcccgta ctctgatcgt cgggatcatc cccgccccaa
      121 gattaaggca gagtgtttcc cgaatcttct cccagccttc ctcgtcggcc acttcggtga
      181 ccacggctcg atctccctca gccaggtact caaaaagatg gagttcctgt gccagctcac
      241 gggtgagata ccgtctgata aaggaacggt ccgattcaat ctcccgggcc tcaaagatct
      301 tggcaatccc gaaacggcgc tccaaatcgg taaagatcga aaatcccaga tgataagggt
      361 ttaagccgcc gggaatcggg cggatcacct ggtgatggcg tttaagaaat tcaaaatgga
      421 aatcggccga cagttccagt tgattcaaga tccggtagtg ccaaaagctg gcccaaccct
      481 cattcatgat cttggtctcc atctgcggca ggaagtattc ggtctcggtt cggacgatcc
      541 ggagcagatc ccgttcccac tcttcgagcc gtccatactc ggcgatgaac caaagtaaat
      601 cttcctccgg taccagcgga attttattca gatccggcgg tgtttcggaa gaatccttat
      661 tcccctggtc ctgccggatg cggcgatagt atgcctgaag cagtcgttcc ttttgttcgg
      721 ccggagtttc gggccgcctt ccgggagtcc ggttggtctg ataacgcagc gcgtgggcgg
      781 cgtcgagaat ccgttccact ttgaaatacc cgatactggg atcctggata taaccgcgga
      841 ctcgatcggc atgattctta aaggtctcga tggtcaattc cggccgggtc atcttaaaaa
      901 gacggttatt cttaaaaaag tcgttatgcc cgtatacgtg ggcgatggtc aaaatctgga
      961 gcaacaaggt attgtcctgc atcaaatagg ccaggcaagg attggagttg atcaccatct
     1021 cgtatggaag accggtcagg ttatagcggt aaaaggtatg ttgccggtcg aaggccttgc
     1081 cgtaactcca gtgcggataa tggctgggca ttccggtata ggcctcgtac ccaagcatct
     1141 cttcataaga acagatctca aactcctggg ggtaataatc aagccccgcc ttttgaacgc
     1201 aatcctcaat ccgctgattc catgtttcca actcggcaag agtgaattcg ctcatgggct
     1261 gctcctaaca ttttagtctt gttctcttgc cagcatatcc cggagggccg gccagatatc
     1321 ctcccgtccg gcaatgctga ccaaggccag attcttggca tcgatgcctt ggcgatatag
     1381 attacggatc gtactggaac cggtcatgat ctcgccgtaa ccgaagagat tgcatacctc
     1441 acacaatcgc cttgctagag ccaaagcttt ttcgttatcc tcgtcccagt tatctccgtc
     1501 actgcaatga aaagcgtaga tattccaatg gataggcgga tagcgttcct cgatgatctg
     1561 cagcgccttt tcatatccac tactgatata ggtgccgccg gattcaccct tgtgaaagaa
     1621 ttcagcctcg ttaacctcca ccgcctgggt ggtatgggcg atgaagacca attccacccg
     1681 atcatacttc atccggatga actgatacaa caggaaataa aaactccggg ccagatattt
     1741 cttggtctga tccatggatc ccgaggtatc catgatacag attaccaccg cattggaatc
     1801 cggggcgtat tcctcacgaa cccgatggta gcggagatca tcctcccgaa aggggaaacg
     1861 cccgcttgct tctgaagatt ccacccccgt tccagacggt aagcctgctc ctactgaatc
     1921 catggcaccg gttacatcct cttgagtggt atcgaccggg gccggttccg gggaaccggc
     1981 tcccgtatcc ggcggctccc gccttgttcc ttggcgccgc ttgatcttct caataactgc
     2041 ccgtttcttt gctagacggg gcggggtgcc cttatgctgg tacccgcttc gttttttccg
     2101 tgtaacggtc tcgatggctg ccagctgctt tcgttccatg aaagggagat tgagatcatc
     2161 aaagaggtaa ttgatcagtt cctccatggt gatctcggtc tcataaatat cctccccggc
     2221 ctcgttcccc gcgccctgac cgcccttgcc ggcaccttgt ttcccgccgt tttgaagcac
     2281 atcgccgcgg gcctcatctc catcgccggt tcccactccc ggccggttcc ggccgtagat
     2341 gaactgatac tcttttaaac ccttgatggg gatcttaacc tttttcgtcc cgctctgtcc
     2401 gataatgctc tcctcggcga cgatctgccc gatgtttttt ttgatcgact cttccaccaa
     2461 ttgcctgtga cggcgccgat cttccgcagc acgccccgag tccccagccg tttcccgaaa
     2521 gatggccatc ggattccctc cgtttttcag tctttccaaa gattattggc ggcgtatttc
     2581 aggaccacat tgcagcaatg atcgcagtaa ccgttctctt tcatctcagc caccatcgcg
     2641 ttgtactttt cattttgttc cttatcccgc accttcgctt gggtgatgac tcttgagatt
     2701 tcccggaccg aagcggtcag ttttttctca attgctacct ttagcggttc atagctctga
     2761 tagtcgaggc taccaccgtt gcggaggaca aagaacatat aggccgtcac atcttgccgg
     2821 aatcccctgg ccgcgctgcc gctgatcccg atctgttcct cgatggattg gaggaacttt
     2881 tcatccggct ccaattcctc cccggtgttt cggtccttga tcttggtccg gttggcgtag
     2941 gcctcggcat gatcaagata gttgttgaag agatcctggg cttgttcccg gtagccgtgg
     3001 ataaaagcct tagttacctc caactcaagc agcttatgat attccttacg gattgtatct
     3061 tggagaaatc ccagataacg cttgcgttca tcgtcaggca acgatagatc cttcactgat
     3121 ttaacaagtg tctccataac cgccaaggga ttgatgcaac cacattccga ttccgtcaag
     3181 gtcatatcca gagccttcat gatgaagcgg gtggagatcc cggtcattcc ctcgcggttg
     3241 gtggcctctt cgcgcaattc gttgatgtcc acccgcttgg tattgccctg ttccaaaatt
     3301 tcctcaccat tatagatctt aagtttggta aggggatcta ctttggatga gggcgaaagc
     3361 cttgtcaaaa tggcgaacat cgaggccatt tcgatagtat gcggagcgat atgcgcgctg
     3421 aagttgctgt gttttagaag tttctgatag atcttgatct cttcgtttag ctccaaacaa
     3481 tatgggacct caatcttgac aatccggtcc agaatagcct cattggtatg atcggacttg
     3541 aacttattcc attccgcctc attagaatgg gccaaaatta caccgtcgaa atagatcatc
     3601 gctcccttcc ccggagaagg gatcgacttc tcctgagtgg cggtgatcat ggtatgcaaa
     3661 tattcgatct cattcttgaa gacctcgata aattcgacga ctccccggtt tccgacatta
     3721 aaggcaccgt ttagggcgag cacccgcggg tcgtcctcgg ggtaaagatc catttttgat
     3781 atgtccaccg aaccgatgag taccgaggtg tcctggttat tgggatctac cggcgggacc
     3841 actccgactc ccttacggga acggatcgaa aactccaccg tctctatagg aaaccgctca
     3901 tactccccct gatactcgtt tttcaaaaga taccggcagg acggacagag atccccctcg
     3961 atatgcactt tgagcaattc ctcaaacttc gcccgcaaat gcttggggat gagatgcaaa
     4021 ggttcctcac gcatcggaca acctttgatc gcatagattg gtggcgccga ttccagtgcc
     4081 cgttttaaat gctccatcaa agaggacttc ccggcgccga ccggtcctac cagatacaat
     4141 acctgacggc tttcttcacc ctgcatggcg gcggcatgga aataccgcac cagcttcatg
     4201 atggtccggt cgattccaaa aaaatccccg gtgaaaaagg gatactgctt aaggatatca
     4261 ttaccgtata tccggcgcaa tctggggttt tcctcggttt tgatcacatt gaccccagga
     4321 tccgtgatca aatggtacat ccgctgatgg gccagttgca ctatgcccgg attggctctg
     4381 atgatatcca ggtaatccag aaacgtgccc tcgaaatgtt gatgttttct ttcggaacgg
     4441 tcttttttga tcaaatcggc gatctgagat tccattagcc gcacctccca aaagtaggcc
     4501 cgtaatgctt ctttcgtgat gaacaaattg gatacatata ggggtttacc tgctgaaaaa
     4561 ggaggtttcc aatagttcac taccgcctca atatattcta ttaaacgagg aaatttttga
     4621 agccagaaat tagcattccg gtgatgaaaa gggccaggag cggggaagtg gggaatagga
     4681 attttgtatt acgacgcgaa taatagtaga ttacttgcgc aaaaagtctc accgggccgg
     4741 attgccgcca tccaaagaac cgttctctgc gatccggttc agggctgaaa ggaggatccg
     4801 tttatggagt ttgaacgcgc ctcttcgggt ttaactggcc ttgaccgagt tctggatgat
     4861 ttgagattgg gtgataacgt cgtctggcaa gtggatgaca tcgccgatta tcgtcatttc
     4921 gcccgggctt ttgccgcagc ggcactcaag tcccataaga aactggtcta tttccgcttc
     4981 ggccggcatt caccactcat tgatccggcc ccgggtgtgc ggctggaacc ggtgaatgcc
     5041 gcacgcggct ttgaagcctt ttctgcggcg attcgcagtg tcatcacagc tgaaggcgaa
     5101 ggggtgtatt acgtctttga ctgcctttcg gaattgctct ccgaatggtc aaccgacttg
     5161 atgatcggca acttcttcct ggtcacctgt ccctacctct ttcacctgaa aacaattgct
     5221 tatttcgccg tgctgcgaga ccaccattcc tatcagacca ttgcccgaat ccgcgatacc
     5281 acccagatac tgcttgatct ttatcatgat gaccgtttct acattcaccc gctaaaggtc
     5341 tggaaacggt attctcccac gatgttcctg ccccatgtgg caccagacgg gagtgacgtt
     5401 ttcgttcccc tgaccaccag cgccgacgcg gtgcaattgt ttgcttcccg caaaccccgg
     5461 ggaggactga gtcatcccgc ccgaagactt gattattggg atcatctctt tattcaggcg
     5521 gccgaaacaa ttcaagattc ggccggagat tcccggactc ttgcgaaacg ccggcaatac
     5581 ctggataaac tcctaaaaat gatgattagc cgggatgaaa tgatcctcac tctggcccgc
     5641 cggtttctca acattaacga tttgttgaaa atcaaggacc gcttgatcgg ctcaggtttt
     5701 atcggcggaa aggcggttgg cttgattctg gcccgggcca tcctggccgc cagtaccaaa
     5761 cgcgactgga atatgctctt agagccccat gactcttttt atatcggttc cgacgtttac
     5821 tacacctatc tggtggagaa tagttgctgg cagctgcggc aggaacaaaa gctgccggag
     5881 aactattata ataccgcaac aatcctccaa gaaaaactgg aaacaggcca atttccggag
     5941 ataatccgcg atcaatttct cgagatactg gagtattttg gccagtctcc gatcatcgtc
     6001 cgttccagca gcttacttga ggacagtttc ggaaatgctt tcgcgggcaa atacgccagt
     6061 gtcttctgta ctaatcaggg gaacttgacg gaacggtacg atcggtttat ccaggcggtc
     6121 cgccaggtat acgcgagtac aatgaaccgg gacgccctgg cttacagagc gcaacgaggc
     6181 ctggcgaatt gcgatgagca aatggctttg ctggtacaac gggtttccgg gggacaccat
     6241 ggggaatact tctttcccga tctggcggga gtggctttat cccacaatct ctatgtctgg
     6301 cgggaggaga tgcaacccga ggccggaatg ctccgtttgg ttttaggact gggaacccgg
     6361 gcggtagacc gggtggatga cgattacgca cggtttgtgg cgctcgacca cccgctgctc
     6421 cggaccgaaa ccagttccga agagatccgg actttctccc aacacgggat ggaccttcta
     6481 aacctcgatc taaaccagcc ggagaccatc tctatccagg atctgattaa ccggaacctc
     6541 atcggacaac tgccgctttt cgcgactccg gacatttcgg atgactgctt cccggatctg
     6601 ggatttccca gcaggccttg ggtgttcaat tttgatccgc tgctcggaga gacaccattc
     6661 ccccgggtga tgcaggagtt gctccaaacc ctgcaggagg catacggtca tccggtcgat
     6721 accgaattca ccgtaaactt ccgctcgtcc cgggattttt ccattaaccc cgaggaaaac
     6781 ccttcaaacg ggatttcagg ggaaaaaaat gaaaatccgg caagcccaag tatccgcctc
     6841 cctcaggagg cgccccccga gaggatgcag acgaattttt atattaactt gcttcaatgc
     6901 cggccgctgc aggtccgttg caaccagcaa ccagtggcat taccggatta tatcccgggt
     6961 gaacgggtta ttttagagag tcacggtcat ttcatgggcg gtaatcagga acttcagatt
     7021 gagcgattca tctatgtggt gccgcaaatg tataccgggt taagtatcag cgatcaatac
     7081 caagtggcgc gggtcattgg aaagttaaac cggatgatcc caccgcagga tgaatcacct
     7141 acagtcctac tgggaccggg ccgctgggga acgagtacac ccagtctggg tattccggtt
     7201 tcattcgcgg agatcaatca ctttgcggtc ttgggagaac tggcttttca aaccggaata
     7261 tcaaccgggg gtaggcaggc aggtatgata ccggaattgt cctatggcac tcacttcttc
     7321 caggacctgg ttgaaaccgg catcttttat gtggcactca atgaagcagt ggagggtacc
     7381 cgtgtcaatc acgagttttt tatgaaccat cccagtcaac tcttgcgatg gttggcggat
     7441 gcggaacggt ggcggagtat catccgggtg gtggagtttt ctgccgctaa aaccgggttg
     7501 ggtaggaatg aaatactccg gctccacgcc gacgtatccc gaaacaagat gatatgttgg
     7561 gtggaatcct aaacatattc atccctgtga tatgagcatg cttgtggata catgttgttg
     7621 tggatacatg tttgtggata catgccgcgg catgtatcca caattcacac aattcatgtg
     7681 agatatttat cgttgaaatt tctgaatgaa ttcgctgatc ttcttggaag gatcgaaaag
     7741 tgaactgatg gattggtcat gatttagcaa ttccagcaga taggcgatga actgggacat
     7801 atccacgttc acgaaccagg gttcatcttt gaggtcctct ttttgaaagg tcaggttggt
     7861 ggcatatacc cggttaagta gaccctgttg atagccttca cggaactcct gtaacccttc
     7921 ggtgaaaagg gcaaatgtga cagcgaaaaa gatccgacgc gccttccgac gtttcaattc
     7981 tttggcgata tcgagaatcg actcacccga ggcaatcata tcatcgacga tcaggacatc
     8041 cttcccctcg acttcttcac cgaggaactc atgctggatg atggggttct tgccatttac
     8101 cacccgggtg taatcgcggc gtttataaaa cagccccacg ttcaagccca agacattggc
     8161 atagaagatg ctccggctca ttccgccttc atcggggcta atgatcaaca tctgggactt
     8221 atcgatcacc acatcctgct ccttttcaag cagggtcttt aaaatctgat aactgggaag
     8281 caggttgtta aaacccgtaa gtggaatggc gttttgaacc ctggagtcat gggcgtcgaa
     8341 ggtaaagata ttatcgacct taagccgctc cagatcttgt aaggctactg cacagtccag
     8401 tgattcccgg gattggcgcc ggtgttggcg cccaccatat aacagcggca tgatgactgt
     8461 aatccggcgt gctctgccac caatggcagc aatcacccgc ttgatatcct gaaagtgatc
     8521 gtccggactc atcgggcatt ccaggccgaa catcttaaaa cggcaattgt aattgccgac
     8581 atctgcgata atataaatat cggaaccgcg gactgtctga ttgatcaccg ccttgcccga
     8641 gccatccgca aaacggatgc aatcgcaggg aatcaggaat gaaccgggca taaagccggg
     8701 ttccgcgacg taatcgggat gatgttcaag aaagctccga cgctgggcca gcagttgttg
     8761 atcgatcagc cccgccaatt ctctgctgct tgccaaggga ataatcccca gcggcgcaat
     8821 aggcatccgg cgcgtaaaga tattcttcca ttcgggtgaa ctcataattc tcggctccaa
     8881 tctctcatgt gtatataatt atccttcaaa agtttgcttt tttagcagac tttgcggata
     8941 attttaaatt gcggcttgcc gcaatttgtt atcaacaaaa acaatcgacg ataccaacta
     9001 cctttttcct gccatcatca cgtttctttt atcgggtttg atcgcaactc cctaaaaacg
     9061 tatttaactg atatacaagg taacggcata tgttgcattt aatgagccaa aacttccggg
     9121 ttcgttttag tttggcggca gttccgccaa tcgtatgagt atcttgtcaa aaaccgagat
     9181 cgaccttttt ggccaccggg attcgccggc cgcccccgaa agccttggtg gtcaccttca
     9241 agcccggtgc cgcttgtttt cttttgtatt cattacgatt taccgcccga atcacccatt
     9301 gcacgacctc aggcgcgaaa tcccgtgcga taatctcttc agcagagtaa ccttcctcga
     9361 tatagtaatg aagtatctga tccaaaatct catacggagg aagagtgtcc tcatcgcgct
     9421 ggtccggttt gagctcagcc gacggcggct tcgcgatgac ggtttcaggg atgatctcct
     9481 gatcccggtt gataaagcgg gccaattggt ataccatcgt tttgggaaca tcggagatga
     9541 cgctaagccc gccgctcata tccccgtata gagtgcagta acccaccgac agttcacttt
     9601 tgttcccggt ggagagggcc aagtaaccga atttattgga gaaggccatc aggatattcc
     9661 cccggatccg tgcctgaatg ttctcctcgg tagtgtcatg ttccttgcca cgaaaatgat
     9721 tctccagggt atcgaggtag gaatggtaga tattcgtaat cggaatcact aagagctcga
     9781 tgccgagatt ctcagccagc cgttgggaat cagcgatgct gcctgcggaa gaaaacgggg
     9841 aaggcatggc aatacccagg acattctctt tgcccagggc agttgttgcc aaacaacagg
     9901 tcagtgcgga gtcgatccca cccgagagtc cgatgaccgc ccgcttaaaa ccgcatttat
     9961 aaagataatc ccggataccg agcaccaatg ctttatatac cgattcaatc ttctcctgag
    10021 gctgataaaa ccgggcgacc ccggcgcgat ccatatccac ggtctgaatc cattcctcaa
    10081 aaaacggccc catcgtaagc ggattgccgt ttttatcaaa gcacatactg cccccgtcga
    10141 agatcaactc atcattaccg ccgatttggt tcacaaatat aaagggaatt ccgtgtttcc
    10201 tggcatggtt gcggataagg ttatagcgta actcgtcctt gccgatatga aagggagaag
    10261 cggagatatt caggagtagt gtcgcgtctt gcgcggcaag ttccgttatg ggatcgaaat
    10321 cgtacatcat ccgggaaggc caaaaatccg gatcattcca ggcatcctca cagaccgaga
    10381 ttcctaaccg ttcggcctta aaagggaccg tgacaatttg cggcgccgga tcgaaatgac
    10441 gcgcctcgtc aaatacatcg tacgtaggta acaacgattt aggctgatta tgaataatct
    10501 ggccccggta aatcaagatg gccgagttat acagcccctt ccccacatga cccccggtgg
    10561 gagtgggtgc gcccaccagt atcccggtct tgggatagcg ggccgagatt tgtgccagcc
    10621 ttttcaagga atcgccgact tgccggataa acccgtctct ttccaataaa tcctgagggg
    10681 ggtagcctac cagaaacatc tcgggaaaaa caaccaaatc ggagtcttcc cgattggccc
    10741 gatcccaaac ctctataatc ttttgggtat tcccggcaag atcaccgatc accggattca
    10801 attgtgcaat tgtaaccttc atcggatgaa tgcggcctca cgttcactgc ttcgcttcat
    10861 tttcaaattc aggtgtatcc tttaaaatcc aaaccgccgg tgcgattgca tacgccccaa
    10921 ccatccggct gaccaggttt gtttggataa aaagctttaa ggaaaacatc cactttggta
    10981 actattccgc aagacatcta attttcctgc ctttgcttct ttaacaaaag attacgtcca
    11041 ctctagtcta gtggaacacg attccggtcg ccctgccgca tctggcgcag ctttcaccgg
    11101 tcaggacacg ggtctcataa ccatgccgct cgattaacaa gttaccgcac tcagggcagt
    11161 aggtatcgct tccgcccgga gcattgccca ggtaaacata gcttaaatac tcccgggcga
    11221 tatcgcgggc acgggccagg gtttcaaggg gggtcgccgc ggtggtgagt ttataatttg
    11281 ggaaataacg ggagagatgt agtggaatct ctgggtcgat ggaaccaatc caccttgcca
    11341 gttcccgaag ttgatccggc gcatcgtttt ccccgggaat gatgagggtg gtcaattcca
    11401 ggtggatccg ccccgccgcg gatttgatat tctccaatac cggttccaat cgtccatggc
    11461 agagtttccg gtaatattct tcggtgaagc ccttgagatc aatgtttgcg gcgtcggtcc
    11521 acttcaacag ttcctgccag ggcccggggt tcaaaaagcc gttggtcacc aggacgtttt
    11581 ttaatccctg ttcatggagg agcggcatgg tttcgatcag aaattcaaac cagactccgg
    11641 gttccgaata tgtatacgcc accccgatac tgcctgcttg tttttcggca ctcttcgcca
    11701 attctgccaa ttccgcggga ctgaccaacc ttgtgggagc gccgtcgcct tttgatatct
    11761 cccagttctg acaaaattca catgccagat tgcagccgat ggttcctgcg gaaaaaatgg
    11821 cgcttcccgg aaaataatga tataacggtt tcttttcgat cgggtccagc gccaggctgg
    11881 aaacctgacc gaaattagtt gtggtcagct tttgatcccg gttgatccgg actttgcata
    11941 acccggttcc tcccgacccg atccggcatt cctggggaca caacccgcaa ccgattaaag
    12001 tatctgaaac cggctcataa aaccgggcct catgaacttt tgtttcttga ttactcacct
    12061 aaatcacctc ataatacgac tacgggagaa tatattccac ctgaacttga taaaaaccca
    12121 cccgacgatt taaatcaccc cagggtgccg cgcgcagaac ccattggaat ggtttcggtt
    12181 tccaagttag cgtggtgaac cacccggtgc agcttgaatc atccgcaaag atcccgtcgt
    12241 aatcaagccg gatccaaaac caccccacct cggaagcgaa cgtttctccc
//
LOCUS       DASVMB010000444         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_277399,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000444 DASVMB010000000
VERSION     DASVMB010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277399"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VF531_12455"
     CDS             complement(<1..1112)
                     /locus_tag="VF531_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX7714817.1"
                     /translation="MRPLFTNRYRHFKRYREIANVLSRHGLGYLLETTGLDNLFRLPW
                     QKREPQGPPSSIAQRIRMVLEELGPAFIKLGQVLSTRPDLLPAVYITELELLQDQVPP
                     LSFPEIKASIEAELHQSLEQLFASFDTTPLASASIGQVHQARLTGGEHIVVKVQRPGS
                     SRKVQTDLEILQDFARIVTTRTEWGKLYQLQDLVSEFAQTIREELNYTFEAHNAERLR
                     RNFLNDPQVRIPKVYWNYTTPRILVLEYCAGVKITAINELGEREIETRTVVHRLARAF
                     FKQLLIDGFFHADLHPGNLAVTDDGAILFMDFGVMGRLDEDLRQKLGAMLLAVIRRNA
                     DGILRLLVAIGSVSPKVNRRKLRRELARLMDRYATLP"
     gene            1349..2068
                     /locus_tag="VF531_12460"
     CDS             1349..2068
                     /locus_tag="VF531_12460"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7714818.1"
                     /translation="MKNRFGGFVILGVSLILSSVCFGIFFYQSRMPEKTIRVVGAATQ
                     RFDSDIIKWRITIFRNAGLNNLKEGYSRIKDDMRSFVALMRENGIGAKDITVQPVNAN
                     PVYSQYEKGGNPSGYSIIQSAYLISRDLNKVEALALNPSLLADRGIMLQSSNLEYYFS
                     KLSTVKKEMLAAATLDARKRATEIAKSSGSRIAKIESARVGVFQITEPYSTEISDYGI
                     YNTATRQKDITVTINVVFDIK"
     gene            2395..>3263
                     /locus_tag="VF531_12465"
     CDS             2395..>3263
                     /locus_tag="VF531_12465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7714819.1"
                     /translation="MPEFWESVRLRFLNRKSRANDGAELYLSGLDRNIRINTWHGVFN
                     IAALDIVIPFTSIFAMKLGASAFEVALLSSAPAAVSLVAMIPGAHFIDRSDRKKRLTF
                     LFMLAHRIFYLALTCVPFFAPSRRTLIFVVLIAIMNFPGAISNVAWQSLISKIIPPER
                     RAEAFAARNKIMNVVGTAVTLMAGLALDRMAFPLGYQIIFGIAFVLALIELKVFNSLD
                     ESVTRQEPAAVPLEPAHGVPEQSTLKQRLAAIFKEKRFVRYTLASILFYFGWQIAWPL
                     FNWYQVKELGANNV"
BASE COUNT          761 a          783 c          894 g          825 t
ORIGIN      
        1 ggcagcgtgg cgtaccggtc catcaaccgg gccagctccc gtcttaattt acgccggttg
       61 acctttggag agacgctgcc gatggccacc aataaccgga gaatgccgtc ggcattccgg
      121 cggatcactg ccaaaagcat cgcgcccaac ttttggcgga gatcctcatc cagtcggccc
      181 atgaccccga aatccatgaa aagaatagcc ccgtcatcgg tcactgccag attgccggga
      241 tggagatcgg cgtgaaaaaa tccgtcgatc aggagttgtt taaagaaagc ccgggccaag
      301 cgatggacaa ctgtccgggt ctcaatttcc cgttctccca gttcattgat tgccgtgatt
      361 ttcactccgg cacagtattc caggaccagg atccggggtg tggtgtaatt ccagtacacc
      421 ttggggatac gtacctgggg atcgtttaag aaattcctgc ggagccgttc ggcattatgc
      481 gcttcgaagg tataattcaa ttcttcgcgt atcgtttggg cgaactcgga gaccagatct
      541 tgaagttggt aaagtttgcc ccattcagtg cgagtcgtta cgattcgggc gaagtcctgc
      601 aggatctcca ggtctgtctg gactttgcga ctggagccgg ggcgttggac cttgaccact
      661 atgtgctcac ccccggtgag ccgggcttga tgaacctgac cgatggaagc cgaggccaga
      721 ggggtcgtgt caaaggatgc aaaaagttgc tccagggact gatgcaactc agcctctata
      781 ctcgccttaa tctccggaaa tgacaaagga ggcacctgat cctggagcag ttccagttcg
      841 gtgatgtaga ccgcgggcaa aagatcgggc cgggtgctta aaacctgtcc cagtttgatg
      901 aaggccggac ccagttcctc aaggaccatc cggatccgtt gggcgatact cgacggtggc
      961 ccctggggtt cccgtttttg ccaaggcaga cggaacaggt tgtccaggcc ggttgtttcc
     1021 agaagataac ccaggccgtg ccggcttaat acattggcga tctcccggta acgtttgaaa
     1081 tgccggtatc gattggtgaa aagtggtctc acgtttgcca ccgccgtgtt cgtatcctgg
     1141 atattataag ccggatcgga cggaaatatt ttgctgcgac gccgcatata aaaggagaat
     1201 gggaagacga agtttttttg gtttgactat attgcaaagt gttccatatg tgcgtatcat
     1261 tgtgatcaat caagaaaaaa atgaaaaacg atttgtattg ttaatcgccg aatgaaagaa
     1321 tgatgatggg aggaaagggg gaatcacaat gaagaaccgg tttggcggat ttgtgattct
     1381 gggtgtcagt ctgattctca gttccgtttg ttttggaatc ttcttttatc aaagccggat
     1441 gccggagaag accatccggg tggtgggagc ggccacccaa cgtttcgact cggatatcat
     1501 caaatggcga atcaccatct ttcggaatgc gggcctcaac aatttgaagg agggctactc
     1561 ccggatcaaa gatgacatgc gatccttcgt agctttgatg cgggaaaacg ggattggggc
     1621 aaaggatatc acggttcaac cggtcaatgc caatcccgta tacagccagt atgaaaaggg
     1681 cggcaacccc tcggggtact ccattattca aagcgcctat ctgatctcgc gtgatctcaa
     1741 caaggtcgag gctttggcct taaaccccag tttattggct gatcggggta tcatgctgca
     1801 gtcatccaat ctggaatatt atttctccaa actgagcacg gtcaagaagg agatgctggc
     1861 cgcagcgact ctggatgccc ggaaacgggc cacggagatc gctaagagtt ccggcagccg
     1921 gatcgccaag attgaatcgg cgcgggtagg ggtatttcag atcaccgaac cttattcaac
     1981 ggagatttca gattacggca tctataacac cgccacccgg cagaaggata tcaccgtcac
     2041 cattaacgtg gtattcgata tcaagtaata ggtgccgctt tcggcacggc gatcgaaaaa
     2101 acccgccatc ggctaaagat gattcttaaa tccggccgat ggtttttcat gcggtctgtc
     2161 ctgactatcg gcaggatttc cggatcggat ccggaatttg atatattatt tttccaggag
     2221 gtttgggatg aaaattgagc tttgcccctc tggggcagct tcggcgggca ctttggtcgt
     2281 gagcttactc aattatcact ttctatgcac cgttggtacg ggtataccta tttgattggt
     2341 gcgtcatgag agttagagcc aaattggtaa gcaataggat ggggggcagg taaagtgccc
     2401 gagttttggg agagcgtgcg gctgcgtttt ttaaaccgga aaagccgtgc gaacgatggg
     2461 gcggagttat acctttccgg actggatcgg aacattcgga tcaatacctg gcacggagtg
     2521 tttaatattg ccgccctgga tatagtcatt ccttttacca gtattttcgc aatgaagttg
     2581 ggagcttcag ccttcgaggt ggcactattg tcatcggctc cggctgcggt aagcctggtg
     2641 gctatgattc ccggagcgca cttcatcgac cggtcggatc gaaagaaacg gttgactttt
     2701 ttgttcatgt tggcacacag gattttttat ctcgccctga cctgtgtgcc gttcttcgcg
     2761 ccttcccgcc ggacccttat ctttgtggta ttaattgcta taatgaactt tccgggcgcc
     2821 ataagcaatg tggcctggca atccttgatc tcaaagatca tccctccgga acgacgggcc
     2881 gaagctttcg ccgcgcgaaa taagattatg aatgtggttg gaaccgcagt caccctaatg
     2941 gccgggttgg cgctggaccg aatggcattt cctctcggtt atcagatcat ctttggcatc
     3001 gctttcgtgt tggccttaat tgagcttaag gtttttaata gcctggatga gtcggtaacc
     3061 cgtcaagaac cggcggcagt tccattggaa ccggctcacg gcgttccgga acaatccacc
     3121 ctcaaacagc gcttggcggc aatcttcaag gaaaaaaggt ttgtccgcta tacgctggct
     3181 tcgatcctgt tttattttgg ttggcaaatc gcatggccgc ttttcaactg gtatcaggtg
     3241 aaagaactgg gcgctaacaa tgt
//
LOCUS       DASVMB010000445         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_569364,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000445 DASVMB010000000
VERSION     DASVMB010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569364"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1857
                     /gene="cimA"
                     /locus_tag="VF531_12470"
     CDS             256..1857
                     /gene="cimA"
                     /locus_tag="VF531_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7714820.1"
                     /translation="MPSAVFYDTTLRDGTQGEGVYFSVEDKIRIVKKLDRLGIDYIEG
                     GWPGSNPKDQEFYHLLAGLRLDHAKVVAFGSTRRPYNLPETDKTLNDLLATGTGAVAI
                     FGKSWDLHVREVLKITPAENLELIADSLAYLKSHGREIIYDAEHFFDGFKSNPSYALE
                     TLKAAVRGGAGSIVLCDTNGGILPLEMLEILKQVQAEITLPLGVHFHNDSGMAEACSV
                     MAAQNGIVQIQGTFNGYGERCGNANLATIIPILVLKLGYDSAVKKELPRLTETSRFIS
                     ELANLQHDERQPFVGASAFAHKAGTHVDAVLKNPLTLEQIHSEQVGNKRRFLLSEQAG
                     RSTILNKVREIVPETQKDDPEIVSLTKKLKELENIGYQFEAAEASFDLLIKKAFNLYE
                     KAFTLEGFRIIVEKRGDQPVFSEATIKVAVGNEYEITAAEGDGPVNALDSAIRKALHR
                     FYPEINDISLKDFKVRVMDEKRGTAAKVRVLIESSDGNNSWGTVGVSENLIEASWEAL
                     VDSIEYGILYKHSLMETEHETEPRS"
     gene            complement(1866..2606)
                     /locus_tag="VF531_12475"
     CDS             complement(1866..2606)
                     /locus_tag="VF531_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7714821.1"
                     /translation="MDILAATILGLVAGLSGTMLGGLFILYRSGNSLPRQGGLLGFSG
                     GIMAAVVLFDLWPEAWHFGGFFPTLLGSGIGILMVQFFDPMLQLIPWYRRRKFSKLTK
                     VGILLGVGIGVHNFPEGVALGTTFIASSRITEWLGLALLMAIHNIPEGMVMASAFKIG
                     RVGFGKIILALTLVEVPMALGGTAGGLLGKISQWMIAVSLGFAGGAMALLVAKELLPL
                     AKKFAGAFRVGCGFILGALVGIALVSVV"
     gene            2795..3238
                     /locus_tag="VF531_12480"
     CDS             2795..3238
                     /locus_tag="VF531_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7714822.1"
                     /translation="MEFGGKLMSLLIAAAAGALMAVQGSFNSTLGKTIGWFEGTFSVQ
                     LIGVVTAGVLLWGFRNGSFSKIGEVPWFAWLGGVIGVAIIFGVAVSIPKLGVGMATTA
                     IIAAQLLTAYLIDHFGWFGMETIPFDMIKAGGIILIVIGAKLLMG"
     gene            complement(3240..3605)
                     /locus_tag="VF531_12485"
     CDS             complement(3240..3605)
                     /locus_tag="VF531_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7714823.1"
                     /translation="MGKNIMVVDDAIIIRLMMKKILTEAGYNVIGEASTGAMAIRKYR
                     ELQPDLVMMDITMPEMSGISALKAIRELDPAAKVVICSALGQKKLILEAMEAGATNFI
                     IKPFEPDQVVTAVGQALGA"
BASE COUNT          977 a         1000 c          917 g          880 t
ORIGIN      
        1 caggggcacc tcatctttct tgacaatccg ccatttcatc cacgattcct cctgagatcc
       61 ctcgattccg gatttcacgg acccgcccgg gccgcaaatg aacccccaag ggtttgtcaa
      121 acaacacgac aaccattcca aagtatgact ggaaacaagg cttggtgcat acttatcaag
      181 ttttaattga ctttcagcgg ggcgggcatt aaaataaaat ttaataccta tatgagtcac
      241 cggaggtgtt cccgtatgcc ttctgctgtt ttttacgata ccacgctacg tgatggtacc
      301 caaggcgagg gggtctattt ctcggtcgag gacaaaatcc ggatcgtcaa aaaactggac
      361 cggttgggta tcgattacat tgaaggcggt tggcccggtt ccaaccccaa agatcaggaa
      421 ttttatcatc tccttgccgg tctccggctg gatcatgcca aagtcgtagc cttcggaagc
      481 acccgccggc cttataacct gcctgaaacc gacaaaaccc tgaatgacct attggctacc
      541 gggaccggag cagttgcgat cttcggcaaa agctgggacc tccacgtccg tgaggttctt
      601 aagataacac ctgcagagaa cctggaatta atcgccgatt ccctggccta tctgaagagc
      661 catggccggg agataattta cgatgcggag catttcttcg acggcttcaa gtcaaatccg
      721 tcatacgcct tggagacact caaagccgcc gttcgcggcg gcgccggtag catcgtgtta
      781 tgtgacacca acggcggcat cttgcccctt gagatgttgg agattttaaa acaagtgcaa
      841 gcggagataa cactgccgct cggggtccat ttccataacg actccggcat ggcggaggcc
      901 tgttcggtca tggcggccca gaacgggata gttcagatcc aggggacctt taacggttac
      961 ggtgaacgct gcggcaacgc aaatctggct acaatcattc ccatcttagt gctcaagtta
     1021 ggatacgact ccgcagttaa aaaagagctg ccccgtttga ccgagacctc acgtttcatt
     1081 tcggaacttg ccaacctgca acatgatgag cggcaaccct ttgtaggcgc ttccgctttt
     1141 gcccacaagg caggaaccca tgtggacgcc gtactcaaaa atcccttgac cttggaacag
     1201 attcattccg aacaagttgg aaacaagcgc cgctttttac tctcggaaca ggccggacgg
     1261 agcacgatcc tgaataaagt ccgggagatc gttcctgaaa cccaaaaaga tgacccggag
     1321 atcgtctcac tcaccaaaaa attgaaggag cttgaaaata tcggttacca gtttgaggcg
     1381 gccgaggctt cattcgacct cttgatcaaa aaggcattta atctttacga gaaagctttt
     1441 accctggaag gtttcagaat cattgtcgaa aagcgcggtg atcaaccggt gttttccgag
     1501 gccactatca aggtggcggt gggaaacgaa tatgaaatta ccgctgccga gggcgatgga
     1561 cctgtaaacg ctttggattc ggcaattcga aaagcgttac accgttttta ccccgaaatc
     1621 aatgatatta gcctgaagga cttcaaagtc agagtaatgg atgaaaaacg aggcacagcc
     1681 gccaaagtcc gggtcttgat cgaaagctcc gacggaaaca actcctgggg aacggtgggg
     1741 gtctccgaaa acttgatcga agcctcctgg gaagctttgg tggacagcat cgaatacggg
     1801 attctttaca aacactctct aatggagacg gagcacgaaa cggagccccg ttcgtagatt
     1861 aattctcaaa ccacgctcac cagggcaata cccaccaaag cgcccagtat aaagccacat
     1921 cccacccgga aagctccggc gaactttttt gccagcggta acagttcctt ggccacgagc
     1981 aaagccatgg cgcccccggc aaatcccaat gaaaccgcaa tcatccactg ggagatcttt
     2041 cccaggagtc ctccggcggt tcctcccaag gccatcggta cttctacaag cgtcaaggct
     2101 agaatgatct taccaaaacc gacccggccg atcttaaagg cggaggccat gaccattccc
     2161 tcgggaatat tgtgaatcgc catcaacaat gccaatccca gccattccgt tatccggctg
     2221 ctggcaatga aggtggtgcc caaggccaca ccctcgggga agttatggac tccgatcccc
     2281 actccgagaa gaatcccgac tttggtcagc ttggagaact tacgccgccg ataccaagga
     2341 atcaattgca gcatcgggtc aaaaaactgg accattaaaa tcccgatccc gcttcccaat
     2401 agcgtgggaa aaaatccgcc aaaatgccag gcctccggcc agagatcgaa caggaccacc
     2461 gcggccatga tcccccccga aaatcccaat aaccctccct gacgcggcaa gctgttcccg
     2521 gaacggtata aaataaataa ccccccaagc atcgttccgg agagacccgc aactaaaccc
     2581 agtattgtgg cagccaatat atccatgacc acaccacgct ctcaggacgg attcaggcgc
     2641 acgtgcgcac cggcaatact gatatggaac cgttgcgaat tacgcaatcc tttacgatta
     2701 tatgcctgaa attaactggg atatgtgtga aatggatgat gcagactatt tttcgataag
     2761 atcggttatg atcttgaaga cgggggaatt gcaagtggaa tttggcggaa aattaatgtc
     2821 cctattgata gccgcagctg ctggggcttt aatggcggtg caaggcagct ttaacagcac
     2881 tctgggcaag actatcggtt ggtttgaagg aaccttcagt gttcaattga ttggtgttgt
     2941 cacggccgga gtacttttgt ggggttttag aaacggtagt ttcagcaaaa tcggagaggt
     3001 gccgtggttt gcatggctgg gcggggtcat cggtgtcgcc atcatttttg gggttgccgt
     3061 cagcattcct aaactcggcg tgggaatggc gacgaccgcg atcattgccg ctcaattact
     3121 taccgcttac ctaatcgacc acttcggctg gttcggaatg gagacgatcc ctttcgacat
     3181 gattaaagcc ggggggatta tcttaattgt catcggggcc aaactattaa tgggctagtt
     3241 caagctccaa gggcttgtcc aactgccgtc actacctgat cgggctcgaa gggcttaatg
     3301 atgaaattag tggcgcccgc ctccatcgcc tcgagaatca gcttcttttg acccaaagcc
     3361 gaacatatga cgactttggc agcgggatca agctcccgaa tggcttttaa ggcgctgatc
     3421 ccgctcattt caggcatagt aatgtccatc atcaccagat cgggttggag ttcacggtat
     3481 ttacgaatcg ccatcgctcc ggtggatgct tcgccaatca cattataacc cgcttcggtc
     3541 agaatcttct tcatcatcaa ccgaatgatg atcgcatcat ccacgaccat aatgtttttt
     3601 cccatcaaaa attctcccat ctataattcc ctggttaagg tcaaaagtgg gatatcggtt
     3661 tcttcgatat ccctcatcta aattttcggt cgagaattcc attcacttga tagtcaaatt
     3721 catccttgat gcagttcggg ttgatcctcg gccgcttccg gttcgttaag gatg
//
LOCUS       DASVMB010000446         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_645038,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000446 DASVMB010000000
VERSION     DASVMB010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645038"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF531_12490"
     CDS             <1..299
                     /locus_tag="VF531_12490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7714824.1"
                     /translation="METMKSDPVKLAEMDYQFHIAVVKAAHNDFLNSLLKTVFVLLST
                     KNKESLGVDKFIEMLPIHSEIYKAIENANPAAAEAKMRQHMNLLRKAYQLQDIS"
     gene            373..1884
                     /locus_tag="VF531_12495"
     CDS             373..1884
                     /locus_tag="VF531_12495"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7714825.1"
                     /translation="MAQEAAERLLEMEKISKTFPGVKALDDVKFDLKKGEVHVLLGEN
                     GAGKSTLIKILAGVHKPDCGTIKIHGDPVMNYNPHQAQEMGISVIYQELNLVPYLTVA
                     ENIFLGDEPLLPGTGFIDWKTLNQKAATVLTSIGSDINCKTVVEKLSIAQQQIVEIAK
                     AVSKNAQIIVMDEPTSSLAETEIANLFNLIRRLRGQGAGIIYIGHRMEEVFEIGDRAT
                     VYRDGRYIGTKNIHEVDTDELIRMIVGRELTEQYPYTPRQFGETVLKIENLNSGNKLH
                     NINLQLRAGEILGISGMLGAGRTELAQTIFGALPYDSGTIIINDHPVKIKKPQDAISH
                     GIGLLPEDRKNQGLSLIMSIKNNITLASLKRFLNGRLINTRQEESSAMSYVERIPIKT
                     SSIMAEAQSLSGGNQQKVVLAKWLCSEAKIVIFDEPTRGIDVGAKVEIYKLMTELAQN
                     GGAIIMISSELPEVLGMSDRILVMREGRIVKEMSRAEATQEKIHQASVSSQIN"
     gene            1923..2897
                     /locus_tag="VF531_12500"
     CDS             1923..2897
                     /locus_tag="VF531_12500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714826.1"
                     /translation="MAENSTMKPNLKKAPFDVGKLGLLLVFFLMIIAFSLLSGNFLTL
                     DNFRNVLRQSSILFLVVAGQTIVMLTGGIDLSQGSIISLVSILTAIGLRDLGLIPGIL
                     IGLLVGTLCGLVTGWLVGKANLQSFIASLGMMYVADGFALIANNGQPVSDIPSNIIDK
                     FFWLGGGYVGWLPVPLIIAIIIFAICHIFLKSTRSGRYIYAVGGNEEAARLSGVNTSR
                     VKILIFVISGLLCAISSISLTARIISGQPLLGTDFTMQSLGATVIGGTALTGGRGGVF
                     NAVMGVLFIGFMTNGLNILGVPTFIQRVIIGVVIIISVLLAHVKQKQS"
     gene            3018..>3851
                     /locus_tag="VF531_12505"
     CDS             3018..>3851
                     /locus_tag="VF531_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714827.1"
                     /translation="MKKNWLILIGLLVMVMVSFGGFNQSQAAQKITVAVMLPNVGDNY
                     FQTKWYGYSTEAKKLGVDAILYDAGGYANVNKQITQVEDVIQKHVDAIIYHPCNSEAG
                     VPVIREAIQAGIKVVNDNQPCPCPEIQATIMRDFYSIGRSYAQYLTMKTKGKAKVLIL
                     PGPVGGEQNTQLLNGQKDYYRRFPGIKVLDVQFCDSNSAAGLKIMEDMLQAHPDVQYV
                     TCFADLIAQGVVQALRSAGKKPGQVKVICFNMSDLTQKLFREGWIDAIVVGEPVWMAK
                     RS"
BASE COUNT         1075 a          792 c          950 g         1034 t
ORIGIN      
        1 ctatggaaac aatgaaaagc gatccggtca aattggctga aatggactac caattccata
       61 ttgccgtagt aaaagcggca cataatgatt ttctaaattc cttgctcaaa acggtattcg
      121 tactattatc caccaaaaac aaagaatcat tgggggttga taaatttatt gaaatgctgc
      181 cgattcattc cgaaatttac aaagcgatcg agaatgccaa tccggcggcg gccgaagcaa
      241 aaatgcggca acatatgaac ttgcttcgaa aggcttatca gcttcaggat atttcctaac
      301 catgggttaa ccgatcaagg caagaaatgt ttccggactg attcgggctt taagttaatg
      361 gaggtgacga agttggcaca ggaggcagct gaaagacttc tagagatgga aaagatcagc
      421 aaaacatttc ccggcgtcaa agcgctggac gatgtcaagt ttgatctgaa aaaaggggaa
      481 gtgcacgtcc tcttgggaga gaatggtgcg ggcaaatcga ctttgatcaa gattttggcg
      541 ggggtacata aaccggattg cgggacgatc aagattcatg gtgatccggt aatgaattac
      601 aaccctcatc aagctcagga gatgggtatt tcagtaattt accaggaatt gaatctcgtt
      661 ccctatttga cggtagccga aaatattttt cttggagatg aaccgctatt accaggaacc
      721 ggattcattg attggaaaac tctgaatcaa aaagccgcaa cggttttaac cagtattggt
      781 tcagatatca attgcaagac cgttgtagag aaattaagta tcgctcaaca acaaatcgtg
      841 gagatcgcca aagcggtttc gaaaaatgct cagatcattg taatggatga acccacctcc
      901 tctctcgctg aaacggagat tgcaaactta tttaacttga tccgccgttt aaggggacag
      961 ggcgcgggaa tcatttatat cggccaccgt atggaagagg tctttgagat tggggatcga
     1021 gctaccgttt accgggatgg caggtatatc ggtacgaaaa acatccatga agtggacacc
     1081 gatgaattga ttcggatgat tgtaggccgg gagttaacgg aacaatatcc ttataccccc
     1141 cggcaattcg gggaaacggt cctaaaaatt gaaaacttaa actccgggaa taaactccat
     1201 aatatcaatt tgcaattgcg ggccggcgag attcttggaa tctccggaat gctgggcgcg
     1261 ggaagaaccg aacttgccca gacgatcttc ggcgctcttc cctatgattc cggtacgatt
     1321 atcatcaatg atcatcccgt taaaataaaa aaaccacaag atgcaattag tcatggaatc
     1381 ggattattgc ccgaggatcg taagaaccaa ggactttcat tgattatgtc aattaagaac
     1441 aatattactt tagcctccct gaaaaggttc ttaaatggca ggttaatcaa taccaggcaa
     1501 gaagaatcat ctgcgatgag ttatgttgag cgcattccga tcaagacttc atcgataatg
     1561 gccgaggctc agtcgttaag tggcggaaac cagcaaaaag tggtattggc caaatggctc
     1621 tgttcggaag cgaagattgt gatcttcgac gaacccactc gggggattga tgttggggcg
     1681 aaagtcgaaa tatataaatt aatgaccgaa ctggcccaaa acggcggtgc cattattatg
     1741 atctccagtg agctcccgga agtattgggt atgagcgacc ggattttggt aatgcgagag
     1801 ggccgaattg tgaaggaaat gagccgtgcc gaggccactc aggagaagat acatcaggct
     1861 tctgtctcaa gccagattaa ttagcctcca acactaaagc gaatcaggag tgatgaaaag
     1921 tgatggctga aaattcaacg atgaaaccga atttaaaaaa agccccgttt gatgtgggaa
     1981 agcttggatt actgcttgtg tttttcttga tgattatcgc tttttccctt ctttccggta
     2041 attttttaac acttgacaac tttcgcaacg ttttacgaca atcctcgatt ctgtttttag
     2101 tcgttgcagg gcaaaccatc gtgatgttaa ccggggggat cgatctctcc cagggctcca
     2161 ttatttcact tgtgagtata ttaactgcta taggcctccg cgacttgggg ttaataccgg
     2221 gaatcctcat cggtctcctg gtaggaacct tgtgcggtct agtcaccggg tggctggtgg
     2281 gtaaggccaa tcttcagtcg tttattgctt ccctgggtat gatgtatgtt gcagatggtt
     2341 ttgctttaat cgccaataat ggtcaaccgg tttccgatat tccatcaaac attattgata
     2401 agtttttctg gcttggcgga ggttatgttg gctggttacc ggtaccgcta attatcgcta
     2461 ttatcatctt tgcaatatgc cacatctttt tgaaatccac ccgttccggc cgatatatct
     2521 atgccgtagg cggcaacgag gaagcggccc gtctttcggg agtcaatacc agccgggtca
     2581 agatcctcat cttcgttatc agcggtttgt tatgcgcaat tagtagtatc tctttaactg
     2641 cccgtatcat ttccggtcag ccgcttttgg gcaccgattt tacaatgcag tccctaggag
     2701 ccacagttat tggcgggacc gctttaaccg gcggacgcgg cggggttttt aatgctgtca
     2761 tgggagtgct gtttatcggt ttcatgacga atggtttgaa tattttagga gttcctactt
     2821 ttatccaacg ggtgattatt ggcgtagtga tcatcatttc tgtgttgttg gcccatgtca
     2881 agcaaaaaca gagctaaagt aggttagctg tttttgtctt aataatggtc gatggggaat
     2941 gaaaggggga atggtaacgg ctgtttaggg ggtatgcggt attcgttcaa caagatttgt
     3001 acaaaggaga ggaaaaaatg aaaaagaact ggctcatact cattggtttg ctggtaatgg
     3061 tcatggtgag tttcggtgga tttaatcaat ctcaagcagc ccaaaagatt acagtagccg
     3121 taatgttacc caatgtaggc gacaactatt tccaaaccaa atggtacgga tattccacgg
     3181 aagccaaaaa attgggagtc gatgccattc tctatgatgc gggtggctat gccaatgtca
     3241 ataaacagat cacccaggtt gaagatgtaa ttcaaaaaca tgtagacgct atcatttatc
     3301 atccttgtaa ctccgaggct ggagttccgg ttatccggga ggcaatccaa gccgggataa
     3361 aagtggtaaa tgataatcaa ccttgccctt gccctgagat ccaggctact atcatgcggg
     3421 acttctattc cattggccgt tcatatgccc agtatttaac gatgaaaaca aaaggcaagg
     3481 ccaaagtact tattctaccg ggaccggtag gtggagaaca gaacacccag cttctcaacg
     3541 ggcaaaaaga ctattaccgg cgttttccgg gaatcaaggt tttggacgtg caattttgcg
     3601 acagtaattc cgctgccggg ttaaaaataa tggaagacat gcttcaagcg catcccgatg
     3661 ttcagtacgt gacttgcttt gcggatctaa ttgcccaagg ggttgtgcaa gcgctgcgct
     3721 ctgccggtaa gaaacccgga caggtcaagg ttatttgctt caacatgtcc gacctgactc
     3781 agaaactttt ccgcgaaggt tggattgatg cgattgtcgt cggagagccg gtctggatgg
     3841 ccaagcgttc a
//
LOCUS       DASVMB010000447         8670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_717523,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000447 DASVMB010000000
VERSION     DASVMB010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717523"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VF531_12510"
     CDS             <1..486
                     /locus_tag="VF531_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX7714828.1"
                     /translation="EKAGSVAEAIWKHCVVWHEGREIFAWSLDLKGNSNVYDEPPGSL
                     QLLPFHGFCDAANPVYLNTVAEIRKPEYPYSFHGCNFAEIGCEHAPHPWMLSVANSLL
                     CGRKEQCRDILLKASMDNGIACESIDENTGECVTGAAFATCAGFLAFAIYYAFGKKDT
                     V"
     gene            785..1807
                     /locus_tag="VF531_12515"
     CDS             785..1807
                     /locus_tag="VF531_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714829.1"
                     /translation="MSKVTIKDVAKYAGVSTAAVSYVINEVEGKVSTETVKKITDAIK
                     QLNYIPDFSARSLVKNESKLIGVIIPQTEDYKQLLLENPFYSEMVSGVESKTREYGYH
                     MILSGVDKGQSYLDISIQRNLDGAIIMGIYRENFYEELKQAGIPIVLIDSYINDKYFY
                     RVGIDDAYGGYLATKYLIDSGHRNIALITGSIKKDGVVEKRFLGYKKALEEAGLFYNP
                     DYVFESSVSYQHGLETGRTLSQNLKEITAVFATADLIALGFIRGVKESGKDVPRDVSV
                     IGFDDVSVAKIFIPPLTSVKQDIFLKGATAVELLINAIEAGKDTQSREVLLPLRIVER
                     QTVAKV"
     gene            1860..2759
                     /locus_tag="VF531_12520"
     CDS             1860..2759
                     /locus_tag="VF531_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714830.1"
                     /translation="MIGENKIAKRFTIGLFLLPSLIGLVLFNLIPIIESAVLSFTNWD
                     MLTSPKFIGLENYLNVFTDENSVSALFNTFKFIIGYLPMVLSFGLFFAVLLNRRMKGI
                     KVYRVLFFIPVITSWVAVSIIWRWLLNGQSGLINYFLSCFGIQGPVWLQDFFWAMPAI
                     IMASIWKDLGFVTIILLSGLQNISDEYYEAAEMDGAGGWQQFFKITLPLLSPTVFFVI
                     IISLINSFQLFDQVMVMTNGGPAGSTSTIVEQVFKNAFRSYRMGFAAAQSWVLFSIIF
                     LVTLIQNKLQRRWVVYETNHETK"
     gene            2734..3570
                     /locus_tag="VF531_12525"
     CDS             2734..3570
                     /locus_tag="VF531_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714831.1"
                     /translation="MKPITKRNNRESTKDLAAHILLTLAAVCLVFPFVWMLSGAFKDA
                     LEAVKMPPQLIPSRFNLDNFINIMKYFPIDRFLLNSIFVAVITVALQLLFCSMAAFVF
                     AKIRFRGREFLFLVYLITLMIPIQVTMTPLFIVFSKLQLNDTYLGLILPGVFSAFGTF
                     LLRQHIMTVSDSLMEAAFIDGASYFRVFFQIILPLSKPALATLGVLAFMNTWNSYLWP
                     LIIINSTELMTLPLGLTKLIGRWSTEWNILMAGNVVSFIPMLLVYIFAQNYLIKGLTF
                     KQ"
     gene            3672..4943
                     /locus_tag="VF531_12530"
     CDS             3672..4943
                     /locus_tag="VF531_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714832.1"
                     /translation="MKKFLTPVIAMLMVLLMGLMACNSLGASPVTLRLMQYTASGSQE
                     ETLKAMVDEFQRRNPNIKVKVEVVAFADYFTKLNTAISAGNGAPDVFELGYENFVTYA
                     AKGVLRNMNDVISRDKSFKPEVFKKLAYDAFKYQNKQYGVPEGFSAVVMFYNKDLFDK
                     AKVEYPNSSWTWKEELAAAQKLTDTQNGIWGTYAPIQFWEFYKTIAQNGGSIWSADNK
                     KVTINSKQNVEAFQWMLDKAKEYKVSPPLNDDTFSQPDADLNAFKAGKIAMLRVGIWN
                     FGRFADAPFKWDIALEPGNTRKAHHFFADALVVSKRTKNSDAAWKFLKFISSDPFVAN
                     LRIEKGWNVPALNDEKVMENYYKTTPPESKKVVLQALDTLVLPPVGPIPEKWNDLTGI
                     VGQELDKARLGKIDAQTALNNAQEKIEKLMK"
     gene            5057..7339
                     /locus_tag="VF531_12535"
     CDS             5057..7339
                     /locus_tag="VF531_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX7714833.1"
                     /translation="MSSGYDAWSIEPKNLPLRDQKFYESIFALGNGYMGVRGFDEEED
                     RGTRYELCTYIAGIFDYFHPRFTDMVNTPNFWRTKIAINGENFSNGDECTKSLNMREG
                     TISRRIIWKDKNGNSTLIETVKFLSLYSVHSAVLRVKITPLDYDGEISLETGIDADVR
                     NNLIPDDQMKEEFDLYEFLSEAGQGNSDDHVMYISLKTGKSQFELCEGFGINLTSNGN
                     PVSHEDQLIQKDKYIARKVGFRVKKNQAYILDKFISVWTSRDTVDTLCDTVVDAVGTL
                     LDKGFDQVLTEQRKAWQEKWEVSDIFIDGDDQCQQALRYSIFQLIQTNAENDPKVSIG
                     ARGVMHGRYKGCYFWDTEIFMFPFYLYTNPGAARDLLMYRYFTLPGAEKNARQQNVDG
                     ARFPWMSSIDGTEQCDTWDIGFSEVHITADIAYAVNQYYEVTGDMEFIKNYGLEMLIQ
                     TARYWRSRFTYDQPQDRFNMLFVKGPNEYGGVTCNNTYTTMMAIHNLQTAEKFISLLK
                     QLHPMAWEAVKNKVNFSETEIGIWDEIIAKAVINYDVEKNLYIEDDNFFKLEPIDIRN
                     VKEDDIPLYRKICFDRLQRYRVLKQADVLLLMLLLPNRFSKQEKLAAWNLYEPITTHD
                     SSLSFGTHAAMAARLNLAETAYHYFLKSIRLDLDDNMENTGLEGIHFAGLGASWQAVI
                     NGFCGIGLSNEELVVDPHLPVSWKKIRFKLIYRNCRISFDFTRGNTAIRLEAGDCEEL
                     TIIYQGNRFTLKKGARQIFT"
     gene            7543..>8670
                     /locus_tag="VF531_12540"
     CDS             7543..>8670
                     /locus_tag="VF531_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714834.1"
                     /translation="MQPELVSEQLLANVGDNGRISFNGNDLCMRDVAFTAYYLIKKGD
                     LHKAAAIIKNAAAIIRTGTVHYDEAVFFLWMLGEYLNATGEIEDSPDFSRLIDFDIDY
                     LSGEWMKPQKNWLGNLDEAIYLNNIAIAYGAVQSINNKLQSENARKLLIRMREFMFQK
                     FLDQGRVVSRLGDKEILGDISLIAVPFGLLDAGNQILVESINVLEKELVEQGVRLSRR
                     DLFFGGCIRSDLTCLLSWYYSERGNIARAKSLLEEVEKIWNRDGKLYIVDRASAREPL
                     YFDYDTEKSKGNMEESYLGYVLYAIAQLNIRSKEAMTIDSSESIKIIHMPKGTENPYL
                     RETAARYPHHPEKDDIVVLKMITQPLILSQEAYVEYSLNGNS"
BASE COUNT         2412 a         1733 c         2161 g         2364 t
ORIGIN      
        1 gaaaaggcag gcagcgtggc ggaggctatc tggaaacact gcgtggtttg gcatgaaggc
       61 agggagatat tcgcctggtc cttggattta aaaggcaact caaacgttta tgatgaaccg
      121 ccggggagtc ttcaattgtt accgtttcat ggattttgcg atgccgccaa tccggtttat
      181 ctgaatacgg tggccgagat cagaaaaccc gagtatcctt attcgtttca tggctgcaac
      241 tttgcggaga tcggttgtga acacgcgccc catccctgga tgcttagcgt ggccaatagt
      301 ttgttatgcg ggaggaagga gcagtgccgg gacattttgt tgaaggcctc catggataac
      361 ggcattgctt gtgaaagcat tgatgaaaat acgggggaat gtgtgactgg agcggccttc
      421 gcaacttgcg cggggttttt ggcattcgcc atctattacg catttggaaa gaaggatacg
      481 gtataagagg aaagtatcaa ccttccactt tttgggtcta aaaaatttcg tgtatttggt
      541 gattttcgtg gttaaaagtt tctatctgta cgcctatttt attcacaacg aaacacacaa
      601 aagacacgaa aatgggaatg gctttaattg cggccgttga agtattttat ctatcataag
      661 caagaaggat tttgttgatc aactaaatgg aaaataaaat ttttttaaaa ttccagcagg
      721 aattttctca cttgaggaag aataatttgt caatagttaa acgtttaagt ctggtgggct
      781 gaatatgtca aaagtaacaa ttaaggatgt agccaaatac gccggggtat cgaccgctgc
      841 ggtatcgtat gtaatcaacg aagtcgaggg taaggtttcc acggaaacag tcaaaaaaat
      901 caccgatgcc atcaaacagt tgaactatat tccggatttc tccgccagaa gcctggtcaa
      961 gaacgaatcg aaactgattg gtgtcatcat cccgcaaacc gaggattata agcaattgct
     1021 cttagaaaat ccattctaca gcgagatggt tagcggagtg gaaagtaaaa cccgggaata
     1081 cggctatcat atgatccttt ccggggtcga taaaggccag agctatcttg atatttccat
     1141 ccagcggaat ttggacggcg ccattatcat gggtatttac agggaaaact tttatgaaga
     1201 gcttaagcaa gccggaattc ccattgtatt gattgatagt tacatcaacg acaaatattt
     1261 ttaccgggtg ggaattgatg atgcatatgg cggatacctg gccacaaagt atctgattga
     1321 ctccgggcac cggaatatcg ccttgataac cgggagtatc aaaaaagacg gtgttgttga
     1381 aaagcgtttt ttgggatata agaaggcctt ggaagaggcg ggtttgttct ataatcccga
     1441 ttatgttttt gagagttcag taagttatca gcacggactg gaaacaggcc ggaccctttc
     1501 ccaaaattta aaagaaataa ccgcggtatt tgccaccgcg gacttaatcg ctctcggatt
     1561 catcagaggc gtcaaggaat ccggcaaaga tgtgcccaga gatgtatcgg tgattgggtt
     1621 tgacgatgtt tctgtggcca agatttttat accccccctg acctccgtaa agcaggatat
     1681 atttttaaaa ggagccactg cggttgaact tctgatcaat gcgattgaag ccggcaaaga
     1741 cacacaatcc cgggaagttt tgcttccatt gcgtatcgtt gagcggcaaa ccgttgccaa
     1801 agtataggat tgatcatttt atgttaagtt aaacgattaa cttggaaggg acgaagagaa
     1861 tgattggaga gaacaagatc gccaagcgct tcacaatcgg cttatttttg ttacccagcc
     1921 tgattgggct cgttttattt aatttaatcc ccattattga atcggcggtt ctaagcttta
     1981 ccaactggga catgcttact tccccgaaat ttatcggcct tgagaattac ttaaacgttt
     2041 ttacggatga aaactccgtt agcgcactat tcaatacttt caaattcatt atcgggtatt
     2101 tacccatggt tctaagtttc ggtttattct tcgcagtttt gttgaaccgc agaatgaagg
     2161 gcattaaggt ataccgggtg ctctttttta taccggtgat cacctcatgg gtggcggtct
     2221 cgattatctg gagatggctg ctaaacggtc agagcgggct gatcaattat ttcctgtctt
     2281 gtttcggaat ccagggcccc gtttggctgc aggatttctt ctgggcgatg ccggcaatca
     2341 tcatggccag catatggaag gatcttggct ttgtgacaat tattctcctt tccgggctcc
     2401 agaatatctc ggatgaatat tacgaggcgg cggagatgga cggggccggc gggtggcagc
     2461 aattcttcaa aataaccttg cctttgttat ccccaaccgt tttttttgtg atcattatct
     2521 ccctgatcaa ctcttttcaa ctttttgatc aggtgatggt catgacgaac gggggaccgg
     2581 ccggaagtac cagtaccatc gtagaacagg tgttcaaaaa tgccttccgg agttaccgga
     2641 tgggatttgc agctgctcag tcatgggttc tgttctcgat tattttcctt gttaccctga
     2701 ttcagaacaa actccaaagg agatgggtgg tttatgaaac caatcacgaa acgaaataac
     2761 cgggaaagta cgaaagatct cgccgcccat atcctactca ccctggccgc ggtatgtttg
     2821 gtcttccctt ttgtttggat gctgtccggg gcctttaagg atgctctgga agcggttaaa
     2881 atgccgcccc agttgattcc ttcccggttt aatcttgata attttatcaa tatcatgaag
     2941 tactttccga tcgatcgctt tttattaaac agcatttttg tcgcggtgat caccgtcgca
     3001 ctgcaattat tgttctgctc catggctgcg tttgtgtttg ccaagattcg atttagggga
     3061 agggaatttt tgtttctggt ttatctgatc acgctgatga tccccattca ggtgacaatg
     3121 accccccttt ttatcgtttt ttcgaaattg cagcttaacg atacctatct gggattgatt
     3181 ttacccgggg tattcagtgc attcgggact tttttattga gacaacatat catgaccgtt
     3241 tccgattcgt tgatggaagc ggcattcatc gatggggcgt cttatttcag ggttttcttt
     3301 caaatcattc tgcctttgag taagccggcc ttggccacct taggtgtgtt ggcctttatg
     3361 aacacctgga atagttattt atggccgctg atcatcatca acagtaccga actcatgacg
     3421 ttacccttgg gcctaaccaa actaatcggc aggtggagca cggagtggaa tattttaatg
     3481 gccggcaatg tcgtcagttt tataccgatg ctcctggttt atatattcgc gcaaaattac
     3541 ttaatcaagg gccttacttt caaacaataa taactgctaa aaatcagtct ttgtgaaaga
     3601 gaggtgattg cgattccggt ttttcgttaa ttgtgtgaac tctattacca ataaaaggag
     3661 cgtgaatggg tatgaaaaag tttttaacgc cggttattgc aatgctaatg gtgcttttaa
     3721 tggggctgat ggcctgtaat tcattgggcg cctcaccagt gaccctacgg ctgatgcaat
     3781 acacggccag cggttctcag gaagaaacct tgaaggcgat ggtggatgag tttcagaggc
     3841 ggaatcccaa catcaaggtg aaggtggagg tggtggcgtt tgcggattac ttcaccaaac
     3901 tgaatacggc catcagcgcc ggcaatggag ccccggacgt tttcgagctg ggctatgaga
     3961 acttcgttac ttatgcagcc aaaggcgtat tacggaacat gaacgacgtg atttcacggg
     4021 acaagagttt caagcctgaa gtattcaaaa aattagccta tgatgccttt aagtatcaaa
     4081 ataagcaata tggagtgccg gaaggttttt ctgcggtagt gatgttctat aataaggatt
     4141 tatttgataa ggcaaaggta gaatatccca attcgtcttg gacgtggaaa gaggagttgg
     4201 ccgcagcgca aaagctgacc gatacccaaa acgggatctg gggaacttat gctcccatcc
     4261 agttctggga gttttacaag accattgccc aaaatggcgg cagtatctgg agcgcggata
     4321 acaaaaaggt aaccattaac agcaagcaga atgtggaagc cttccagtgg atgctcgata
     4381 aagcaaaaga atataaagtc tctccgcctt tgaatgatga tactttcagt cagcccgacg
     4441 cggatctgaa cgcctttaaa gcagggaaaa ttgcgatgtt aagggtcggt atctggaact
     4501 tcggcaggtt tgccgatgcc ccctttaaat gggatatcgc ccttgaaccc ggtaatacgc
     4561 gaaaagcaca tcatttcttt gccgatgccc tggtggtttc gaagcggacg aagaattccg
     4621 atgccgcctg gaaattcttg aaattcatct cgtcggatcc gtttgtagcc aatctccgga
     4681 ttgaaaaggg ctggaatgta ccggcgttaa atgatgaaaa ggtcatggag aattattaca
     4741 aaacgacgcc tcccgagtca aaaaaagtgg ttctgcaggc tctcgacacc ctggtcttgc
     4801 cgccggttgg tccgataccg gaaaaatgga acgatcttac cggcatcgtc ggtcaggaac
     4861 ttgataaagc cagactgggt aagattgacg cccaaacggc gctgaacaac gcccaggaaa
     4921 agatcgaaaa gttaatgaaa taatcgcagt tgaaagggat ggttgcagca acttgcagcc
     4981 atcccgcaag atttaaatat cacatacagg aatgaatcga tcggatgatc ttaaatgcct
     5041 gggtgaggag tttacgatga gtagcggata tgatgcctgg agtatcgaac cgaaaaattt
     5101 gccccttcgt gatcaaaaat tttatgaaag tatattcgct ttaggcaacg gctatatggg
     5161 ggtcaggggg tttgatgaag aggaagaccg gggaacccgg tatgagcttt gtacttatat
     5221 tgcgggaatc tttgattatt ttcatccccg cttcaccgac atggtcaata cccccaattt
     5281 ctggagaaca aagatcgcga tcaacgggga gaacttttcc aatggcgatg aatgcaccaa
     5341 atccctgaat atgagagagg gaacgatctc ccggcggatt atctggaaag acaagaatgg
     5401 aaacagcacg ctcattgaga cggtaaaatt tttgagttta tattcggttc actccgcggt
     5461 tttgagagtc aagattactc ccttggatta tgacggggaa atatctttgg aaacaggaat
     5521 cgatgcggat gttcgcaaca atttaatccc cgatgaccag atgaaagagg agttcgactt
     5581 gtatgagttt ttatcggaag cggggcaagg caattccgat gatcatgtga tgtatatcag
     5641 cttaaaaacc ggtaaatcgc agttcgagct atgtgagggt ttcgggatca atttgaccag
     5701 taacgggaac ccggtcagcc acgaagatca gctgattcaa aaggataaat atatcgcccg
     5761 gaaagtcggg tttcgtgtca aaaaaaatca agcgtacata ttggacaagt ttattagcgt
     5821 atggacatca agggataccg ttgatacgct ttgcgacacg gtcgttgatg cggttggaac
     5881 attgctggat aaaggtttcg accaggtatt gacggagcaa agaaaagcgt ggcaagagaa
     5941 atgggaagta agtgatatct ttattgacgg tgatgatcaa tgccaacagg cgttaagata
     6001 tagcattttt caactgattc agacgaacgc ggaaaatgac cccaaagtga gcatcggggc
     6061 cagaggagtt atgcacggcc ggtataaggg atgttacttt tgggataccg aaatattcat
     6121 gttccccttt tatttatata ccaatcccgg ggccgcccgg gacctgttga tgtacagata
     6181 ttttaccctg ccgggagcgg aaaaaaacgc caggcaacaa aatgtcgatg gtgcaaggtt
     6241 tccgtggatg agttccattg acggaaccga acaatgtgat acctgggata taggttttag
     6301 cgaagtgcat attacggcgg atattgccta tgccgttaat cagtattatg aagtgacggg
     6361 ggatatggaa tttataaaga actacggact ggagatgctt attcaaaccg ccaggtattg
     6421 gcggagccgg tttacctacg atcagccgca agatcggttt aatatgctgt ttgtcaaggg
     6481 ccctaacgaa tatggcggtg taacatgcaa caatacctat acgaccatga tggccatcca
     6541 taatctgcag acggccgaaa agtttatttc cctgttaaaa caactgcacc ccatggcctg
     6601 ggaggccgtt aaaaacaaag tgaattttag tgaaacggag atcgggatct gggatgagat
     6661 tatcgctaaa gcggtgatca actacgatgt agagaagaat ctctatattg aggacgacaa
     6721 cttttttaag ctggaaccta tagatatccg gaatgtaaaa gaggatgaca ttcctctcta
     6781 ccggaaaatc tgctttgaca ggctgcagcg ctaccgggta ttgaaacagg cggatgtatt
     6841 gttattgatg ctgttgctgc ccaaccggtt ttcgaaacag gagaaattgg ctgcctggaa
     6901 tctttatgaa cccatcacga cccatgattc ctcgctaagt ttcggcactc atgcggcaat
     6961 ggccgccagg ttgaacctgg ccgaaacagc ctatcattat ttcctcaaaa gcataaggct
     7021 tgatctggat gataacatgg aaaatacggg tctggaaggc attcattttg ccgggctggg
     7081 cgcaagttgg caggctgtga tcaacggttt ttgcggaatc ggcttaagta atgaagaact
     7141 ggttgttgac ccccatcttc ccgtatcctg gaagaaaata agattcaaac tgatttatcg
     7201 aaactgccga atcagttttg attttaccag gggcaacacc gcaattcgac tcgaagccgg
     7261 agattgtgaa gaactgacca tcatttatca agggaaccgg tttaccctta aaaaaggggc
     7321 aagacaaatc tttacataag ttgtagcagg taaaagtctc attacatttt ttctgggtca
     7381 ggacgaccga tttggtattc cgctcggatt atccgtttgg acgcctatca ttggggtctt
     7441 gacgattatt ccggctgttt tattttttaa ttacgcctta aaaagctatg agagcgttgg
     7501 ttcgtaaata ttggggttta tgataaacag gaggttggta agatgcaacc cgaactggta
     7561 tccgaacagt tattggccaa tgttggtgat aacggccgga tttcttttaa cggcaatgat
     7621 ctctgcatgc gggatgtggc atttactgcc tattatctca tcaaaaaagg tgatcttcat
     7681 aaagcagcgg ccattatcaa aaacgctgcg gcgataataa gaaccggaac cgtccattat
     7741 gatgaagccg tttttttctt atggatgctg ggcgaatatt tgaatgcaac cggtgaaatc
     7801 gaagattcgc ctgatttttc gaggttaatc gattttgaca tcgattatct gtcgggcgaa
     7861 tggatgaaac cccaaaagaa ttggcttggt aacctggatg aagcgatcta tcttaataat
     7921 atcgcgatcg cctatggcgc cgttcaatcc atcaacaata aacttcaaag tgaaaatgcc
     7981 cggaagctgc tcatccggat gcgggagttc atgtttcaaa agtttttaga ccaaggaaga
     8041 gtagtcagta gactagggga caaagagata ttgggggata ttagcctaat tgccgttccc
     8101 ttcgggttac ttgatgcggg aaaccagatt ttggtggaat ccatcaatgt gctggaaaag
     8161 gaattggtgg aacaaggagt gcgcttatcc agacgggatc tctttttcgg aggctgtatc
     8221 cggagcgatc tgacctgtct gttatcttgg tattactccg aaagaggcaa tattgccagg
     8281 gcgaaaagtt tattggagga agtagaaaaa atatggaacc gggatgggaa actatatatc
     8341 gttgatcggg caagcgccag ggagccattg tattttgact acgatacgga gaaaagtaaa
     8401 ggaaatatgg aggaaagcta tttgggctat gtcttgtatg ccattgccca gttgaatatt
     8461 cgttccaaag aagcgatgac gatcgattct tcggaaagta tcaagatcat tcatatgccc
     8521 aaagggaccg agaatccgta tttacgggaa accgccgcaa gatatcccca tcatcccgag
     8581 aaagacgaca tcgttgtttt gaagatgatt acccaaccat tgattctgtc tcaggaagca
     8641 tacgtggaat acagcctaaa cgggaattca
//
LOCUS       DASVMB010000448         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_645184,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000448 DASVMB010000000
VERSION     DASVMB010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645184"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VF531_12545"
     CDS             <1..537
                     /locus_tag="VF531_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714835.1"
                     /translation="LCSPEPSPVPGSQAGIPDGQGRNRLRKYHWLEIWRQSWKKVLAV
                     GVGGFILLGLFWPVIHPSENSPLSVTEKFYRDAGTVAAGSAAGNNGMLADFSAVKTER
                     LKLAEDLLSRPVAEVIRLTPLDRNSSRLVIQAELRWWNWSGRVWNSRVSRELLIMQKS
                     RSGWRIVKRRPLEVRSGK"
     gene            complement(545..2593)
                     /locus_tag="VF531_12550"
     CDS             complement(545..2593)
                     /locus_tag="VF531_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714836.1"
                     /translation="MDQQTINMLEFPEIKTRLAALALSGLGRRLAEELTPRDSYSAVS
                     DLLTETSEARQLLDAFGSPPLSGLADLSGPIQKAKSGGILDPLSLQEIGDFLRGCRKT
                     CIYMKQHAEFAPHLTGYAWGISGFEELESEINRCIANGAVTDAASPALRKIRSELSRL
                     RAKIKEKLEQILLSPSSREFLQETVISIKEGRQVLLVKASCKASLPGVVIGSSGSGST
                     LFVEPIAVHRLSNELRTQEGAEEEAIYQILAGLSGAIGEAAAGLERNLEIMGEYDFAF
                     AKARLSRMMRGTAPRLNETGTIRLRGARHPLLGGDPVPLNLTIGPDPDPIWDYRTLVI
                     TGPNTGGKTVTLKTAGLLTLMAQAGLHIPAEAGSELPVFGGVLADIGDGQSITDSLST
                     FSAHIGAIIRIMKQCGPHSLVLIDEVGTGTDPSEGAALAVAILEYIHNRGAITLASTH
                     YPELKSFALTTPGFKNGCMAFDRQELKPLYQLIIGRPGESNALWIAAKLGMPDAVLNR
                     ARREMTGSPGWQPEDEPLLTKALIGPQPSPTAEWAPPCEEKSRETPLGEGLPIKELPF
                     KAELPRTASVKSKATGATETVRPLGIGDRVTIPFLNEQGVVAGNPDSKGRIRVLVKGK
                     KMELPVKRVKRSIPAEELYPDDYDLNIVLLSKEERKMKHRMERGLLHGVERIIPPEEQ
                     "
BASE COUNT          585 a          779 c          786 g          674 t
ORIGIN      
        1 ctatgctctc cggaaccatc tcccgtgccg gggtctcagg ccggtatccc cgacggtcaa
       61 gggagaaacc ggcttcgtaa ataccactgg cttgagatct ggcggcaatc atggaaaaag
      121 gttttagctg tgggggttgg cggctttatt ttgttaggct tgttttggcc ggttatacac
      181 ccttcagaaa attctcccct gagtgttacc gagaagtttt atcgcgatgc cgggacggtt
      241 gcagcgggat ccgccgccgg aaacaatggg atgcttgccg attttagcgc tgtcaagacg
      301 gaacggttga aactcgccga ggatctgctt tcccggccgg ttgcggaggt gatccggctc
      361 acccccctcg atcggaattc cagccgcctt gtcatccagg cggaattacg ttggtggaac
      421 tggtcgggcc gggtttggaa ctcccgtgta agccgtgagc tcctgatcat gcaaaaatcc
      481 cggagcggct ggcggatagt gaaacgcaga ccgctcgagg ttcgctccgg gaaataaaca
      541 atgattattg ctcttccggc gggatgatgc gctcgacccc gtggagtaac ccccgttcca
      601 tccggtgttt catttttcgt tcctccttgc ttagaaggac aatattgaga tcgtaatcat
      661 ccggatacag ttcctctgcc gggattgacc gcttgacccg tttcaccggg agttccatct
      721 tcttaccctt taccagaacc cgaatccttc ctttggaatc aggattgcct gctacaactc
      781 cctgttcatt cagaaaagga atagtcaccc gatcaccgat ccctaagggc cggactgttt
      841 cagttgctcc cgtggctttc gatttcaccg aagccgttcg gggtagttcc gccttaaaag
      901 gcagttcctt aattggtagt ccctcaccaa gtggcgtttc acgcgatttt tcctcacagg
      961 gcggtgccca ttcggccgtc ggagacggtt ggggtccgat aagggccttt gtaagcaggg
     1021 gttcatcttc cggttgccat ccgggactac cggtcatttc ccgacgggcc cggttcagga
     1081 ccgcatccgg cattccgagt ttggccgcga tccacagggc gttactctct ccgggacggc
     1141 cgatgatcaa ttgataaagt ggcttcaatt cctgtcgatc aaaggccata caaccgtttt
     1201 taaagccggg ggtggtgagt gcgaagctct tcaactccgg gtaatgggtg gaagccaggg
     1261 tgattgctcc gcggttatga atgtattcca gaatggcgac agccagggcc gctccctctg
     1321 agggatcagt tccggtgccg acctcgtcga tgaggacgag ggagtgtggg ccgcactgct
     1381 tcatgatacg gataatcgca ccgatatgcg ccgagaaagt gctgagggag tcggttatgc
     1441 tctgaccgtc accgatatcc gccagaaccc ccccgaaaac agggagttcg cttccggctt
     1501 ccgcaggaat gtgtaaaccc gcttgggcca tcagcgttag gagacccgcg gtttttagcg
     1561 tgacagtttt tccgccggtg ttgggcccgg tgatgaccag tgtccggtaa tcccaaattg
     1621 gatccggatc cggaccgata gtcaggttga gcggtaccgg atcacctccc agcaatggat
     1681 gacgggcacc cctgagccga atggttccag tctcattgag acggggcgca gtaccccgca
     1741 tcatccggct gaggcgggct ttggcaaagg caaaatcata ttctcccatg atctccaaat
     1801 ttcgttccag accggcggcg gcttcaccta tcgcgccgct taatccggcc agtatctgat
     1861 agattgcttc ttcttcggcg ccctcctggg tgcgcagttc gttactgagg cgatgaaccg
     1921 cgatgggctc gacgaataag gtgctcccgc taccggaact gccgatgact accccgggaa
     1981 ggctggcttt gcaggaggcc ttcaccagca gaacctggcg cccttcctta atactgatca
     2041 ctgtttcctg gagaaactcc cgactgctcg gggagagcag gatctgttcc aacttttcct
     2101 taatcttcgc ccgtagcctg gaaagctcgc tacggatctt ccgcaaagcg ggactggcag
     2161 cgtccgtaac cgctccattc gcgatacaac ggttgatctc cgattccaac tcctcaaaac
     2221 cggagattcc ccatgcgtaa ccggttaaat ggggtgcaaa ttccgcatgt tgcttcatgt
     2281 agatacaggt tttacggcaa ccccgtaaaa agtcgccaat ctcctgcaag gaaaggggat
     2341 cgagaatccc accgcttttg gctttttgga taggcccgga cagatcggcc agacccgaaa
     2401 ggggcggtga accgaaggca tccagtaatt gccgtgcctc actggtttct gtgagcagat
     2461 cggagaccgc gctatatgaa tcccggggtg ttaattcttc ggccaacctt cggcccagac
     2521 cgcttaaggc caaagctgcc aggcgggtct tgatttcagg aaactcaagc atatttatgg
     2581 tttgttgatc caaagggtat cttcctttca tgacttaaaa attagattgg agaatttgag
     2641 gcggcgtagt tgcgcgcgac cgcgcgcaat tacgcgttcg cgcgatcgag taggaggagg
     2701 cggctagccc ccgtcctctc acaccaccta gcatgcgggt ccgcactagg cggttcgaca
     2761 gttttgacga agtactaagt atctttgagt taagctcata agcccttggg attgccagta
     2821 ggct
//
LOCUS       DASVMB010000449         4211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_495920,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000449 DASVMB010000000
VERSION     DASVMB010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495920"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VF531_12555"
     CDS             <1..654
                     /locus_tag="VF531_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX7714837.1"
                     /translation="NIPIALITEEYLAYLRTIQMLNLEVAGDFLVMAATLMQIKAKML
                     LPAVPSDDETAEFEEDPRLELAQKLIEYKKIKEASLMLQSLEGQQFQIYTRSGGDFAD
                     KQLAQEPEFPKGLSVWDLVGAFKVILDSLEPKPIRAIPQQEVSIKQRMTEVLDLVTGQ
                     GQVLFKTVFNHISTKIGLITCFLAVLELIKLRKIIAFQGELFGDIRLALPSPENLNS"
     gene            758..1336
                     /gene="scpB"
                     /locus_tag="VF531_12560"
     CDS             758..1336
                     /gene="scpB"
                     /locus_tag="VF531_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7714838.1"
                     /translation="MSSLNLVMAKAVIEALIFASSEPLTTKIMAEIIGIDDHTVRQLI
                     ADLIEEYRQTSRGIQIIEVANGYQFSTQSECSPFVEKLQKVPRNVGLSQAAIETLAII
                     AYKQPITKAEIESLRGVSVESSLNTLLEKNLVEEGGRKEAPGRPILYQTTKRFLQYFG
                     LNDLKELPKVPDWIDPGTVKYSNDTLEAGNHG"
     gene            1329..1619
                     /locus_tag="VF531_12565"
     CDS             1329..1619
                     /locus_tag="VF531_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714839.1"
                     /translation="MAEFFLKLFKSPGRDNNYYLGLFLLLLGILSLGVYMLVDFLGIF
                     NGFEPVSFLITFLISGWGFVLLWKSDTDYRFHQAEEKITELEAEIAYLKKMQ"
     gene            1751..2878
                     /locus_tag="VF531_12570"
     CDS             1751..2878
                     /locus_tag="VF531_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX7714840.1"
                     /translation="MFTTAKGRVTGIIRETPVLQEIWVQIADHSQPERAYNYPGLYHR
                     VVVGEKVCLNTTAVNLGLGTGGRHFVIPDPQVEEDRSGPGHIMKLRYTPWQFSVLTAE
                     EDTSPYHQCLEESGGLDGTPVVATPLHSMIPGVILGFNHLWNERHPDKKPRIVYVMTD
                     GAALPIALSDLVRDLKEKALLDLTITAGHAFGGDLESVSIPSALITAKTVGKADLIIV
                     ALGPGIVGTGTPLGFSGVESSWVIDLTVRLGGTALAVPRISQADPRLRHYGLSHHTRT
                     VLQLASNPAKLALSRLIPPDLQQHILEQLRNSGLLNRDDWYWVNDLSAEELFTAYGIK
                     VKTMGRDIHQDTAFFQTSVAAGFLAAQAHDGDLPSLVKIMG"
     gene            2884..3426
                     /locus_tag="VF531_12575"
     CDS             2884..3426
                     /locus_tag="VF531_12575"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7714841.1"
                     /translation="MNKSLIEVPQTKSVVYKGNFLEFHRDTVVLPNGCTTSREYLHHP
                     GAIAAVPVLDDGTVILVRQFRYPTGRALLEIPAGKLESGEEPEECVRRELAEEIGYQP
                     DTIIPLTSIWTTPGFTDEIIHLFYATGLKPCPGQTDPDEFLERVIMTRQELLEIVTKP
                     DLIDGKTIVALSLMELRKLW"
     gene            3585..>4211
                     /locus_tag="VF531_12580"
     CDS             3585..>4211
                     /locus_tag="VF531_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714842.1"
                     /translation="MHLTFNASGAVEGARRGDLVVVVDIIDMSTSAEVALEAGAIAIY
                     GASPSGCKAPVTLNPDRIGYLAGKTALKYKTKLIVVAEPRYGDPEERKKRAEAALMGV
                     ARSGAEVGMIVPNIGSEIGKCADFRGMVVLCVSDTGGVAFDAAYNNGAPAVLTATVTR
                     TPEKKGLEPARFGVERAIDYATKNNCGITVTAASSNSLEDILASQFLVQ"
BASE COUNT         1056 a          981 c         1109 g         1065 t
ORIGIN      
        1 aatattccta tcgccctgat caccgaagag tatttggcct atctccgtac cattcagatg
       61 ctcaatctgg aggtggccgg tgattttttg gtgatggccg cgacgctgat gcagatcaag
      121 gcgaaaatgc ttttacccgc agttccatca gacgacgaaa cagcggaatt tgaggaagac
      181 ccccgtcttg aattggccca aaaattgatt gagtataaaa agattaaaga agcctcctta
      241 atgctccaat ccctggaggg acaacagttt cagatttata cccgttcagg cggagacttc
      301 gccgacaagc agcttgccca ggaacccgaa ttccccaaag gcctgtccgt atgggatctg
      361 gtcggcgcct tcaaagtaat cctggacagt ctcgaaccta aaccgatacg ggccattccg
      421 cagcaggagg tttccatcaa acaacggatg acggaagttc ttgatttagt caccgggcaa
      481 gggcaggtcc tttttaagac ggttttcaat catatttcca cgaaaattgg actcatcacc
      541 tgcttcctgg cagttttgga attgattaaa ctccgcaaga ttattgcttt tcagggagaa
      601 ttatttggag atatccggtt ggcgcttccc agcccggaaa accttaattc atagttcccc
      661 tccatggtgt taaggggtta ttgattcttg ctcattgata cttcccatac cggggaaacg
      721 gtgcaattgt gaaataattt cggcaaaaga aggcgcggtg tccagtttga atctagtcat
      781 ggccaaagcg gtgattgagg ccttaatctt cgcatcttcc gaaccattga ccacaaagat
      841 aatggccgag atcatcggaa tcgatgatca taccgtacga cagctgatag cggatttgat
      901 cgaggaatat cgtcaaacga gcagagggat ccagattatc gaggtggcga atggatatca
      961 gttttctact cagtcggaat gttctccttt tgtagaaaag ctacagaaag tcccgagaaa
     1021 tgtagggctg tcgcaagctg ccattgagac cttagcgatt atcgcttata aacaaccgat
     1081 taccaaggcg gagatcgagt cactccgcgg tgtcagcgtg gaaagctcct taaatacctt
     1141 gctcgaaaag aatctggtgg aagagggagg gcgtaaggaa gcgcccggca ggccgattct
     1201 ttaccaaacg acgaaacgtt ttttgcagta ttttggattg aatgatctca aagagctacc
     1261 taaggttccc gactggattg acccggggac cgttaaatac agtaacgata cattggaggc
     1321 tgggaatcat ggctgagttt ttccttaaac tttttaaaag tccgggccgg gataacaatt
     1381 attatttagg cctttttctc ttgctgctcg gaatactatc cctaggcgtc tatatgttgg
     1441 tggatttttt ggggatcttt aacggttttg aacccgtctc tttcctcatc accttcttga
     1501 tcagcggttg gggttttgtg ttgctctgga aaagcgatac agactaccgg tttcatcagg
     1561 ctgaggagaa gatcaccgag cttgaagcgg agatcgctta cttgaagaag atgcaatgat
     1621 tctctgacaa caatagtcct ttcaggaaga aaagagctcc cgctacatcc ggtagcggcc
     1681 gtcatttttt cttctttttt tctatggaaa caagcatatc gaaaccaaag tttacttttc
     1741 aggaggatta atgtttacca ctgcaaaggg ccgcgtgacc gggatcatcc gggaaacccc
     1801 cgttcttcaa gagatttggg ttcagattgc cgatcactcg caaccggaaa gggcatataa
     1861 ctatccggga ttataccatc gagtggtcgt tggcgagaag gtctgtttaa ataccactgc
     1921 cgttaacctg gggttgggaa ccgggggacg tcattttgtc atacccgatc cccaggtgga
     1981 ggaggatcgt tccggacccg gtcatattat gaagctccgc tatacaccat ggcagttttc
     2041 ggttttgact gccgaggaag atacaagtcc gtatcaccag tgcttggagg aatccggcgg
     2101 actggatggg acaccggtag tcgccacccc gttacatagt atgattcctg gagttattct
     2161 cggctttaat cacctttgga acgaacggca cccagacaaa aaaccgcgta tcgtctatgt
     2221 gatgacggat ggagccgctt tacccattgc cctcagcgat ttggtccgag atttgaaaga
     2281 gaaggcctta ctggacctga caatcactgc cggacatgcc ttcggtggcg atctggaatc
     2341 ggtcagtatt ccatcggcac tgattactgc caaaacggtg ggaaaagccg atctgatcat
     2401 tgtagctttg ggtcccggaa tcgttggtac cggtactcca ctgggtttca gcggagtgga
     2461 gagttcctgg gtaatcgatc tcactgtcag actcggcgga accgcgctgg ctgtaccccg
     2521 gattagtcag gccgatcccc gtttgcgcca ttacgggttg agtcatcaca cccggacagt
     2581 tctgcaactg gcaagtaatc cggcgaaatt ggcattatca cgattaatcc ctcccgatct
     2641 gcagcaacat attctggagc aattgcgtaa ttccggtttg ctcaacaggg atgactggta
     2701 ctgggtgaac gacctgtccg cggaggagtt gttcactgca tacgggatta aagttaaaac
     2761 tatggggcga gatatccatc aggatacggc tttttttcaa acctctgtgg ctgcagggtt
     2821 tttagcggct caagcccatg atggagattt gccgtcttta gtcaagatta tggggtgaat
     2881 cagttgaata aatcattgat tgaagtaccg cagaccaagt cagtcgttta taaaggtaac
     2941 tttttggaat tccaccggga tacggtggta ttgcccaacg gatgcaccac ttcacgggag
     3001 tatttgcatc atcccggtgc catcgcggct gttccggtcc tggatgacgg tactgtgatt
     3061 ttagtacggc agttccgata tccgaccggt cgagctttgc tcgagattcc ggcagggaaa
     3121 ttggaatccg gggaggaacc cgaggaatgt gtccggcggg aattagcgga agagattgga
     3181 tatcaaccgg ataccatcat tcctctgaca tctatctgga ctacccccgg ctttactgat
     3241 gaaatcatac acctttttta tgcgactggg ctcaagcctt gtccggggca gacagatccc
     3301 gacgaatttc tggaacgggt gatcatgacc cgacaggaat tattggaaat agttaccaaa
     3361 ccggatctga tcgacggaaa aactatcgtt gccttatcct tgatggagct acgcaaacta
     3421 tggtagggga tcgaggcatg aaatcttacg atgatgaggc gcggatgcgc gcatagtcag
     3481 gcatctttct cataatatat atcgggagac acgcctccgc cggtttggcc gtctacaccg
     3541 cggtggccat gaaagaagaa aataggtgaa atggtttgaa aacagtgcat ttaaccttta
     3601 atgcctccgg ggcggtggaa ggcgcccgac gtggtgattt ggtcgtagtg gtggatatca
     3661 tcgacatgtc gaccagtgcc gaggtggcat tggaagcagg agccatcgcc atttacggcg
     3721 cctcgcccag cgggtgcaaa gccccggtta cgctaaaccc cgatcgcatc ggttatctgg
     3781 ctggaaagac cgccctaaaa tacaagacaa aattgattgt ggtggccgaa ccccgttatg
     3841 gcgacccgga ggagcgtaaa aagcgggctg aggcggcgct gatgggagtg gcccgttccg
     3901 gggctgaagt gggaatgatc gttcccaata tcggctcgga gatcggtaaa tgcgctgatt
     3961 tccggggaat ggtggtatta tgtgtatccg ataccggagg agtggcattt gacgctgcat
     4021 ataataacgg tgctcctgcc gttttgaccg ccacagtcac acgaaccccg gagaaaaaag
     4081 gacttgaacc tgcccgtttt ggggtggaac gggcaattga ttacgccacc aaaaataact
     4141 gcggcatcac cgtcaccgct gccagctcca attcactgga ggatatcctt gccagtcaat
     4201 ttttggtaca a
//
LOCUS       DASVMB010000450         6440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_130137,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000450 DASVMB010000000
VERSION     DASVMB010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130137"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2559)
                     /locus_tag="VF531_12585"
     CDS             complement(<1..2559)
                     /locus_tag="VF531_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714843.1"
                     /translation="MKSIQRSKKLKSVILGGAIFLLAGTQVLFAYTMQYNFTSPRDPG
                     WPTFSGAAAPAEPVAYIVNGMMQTIYDAEKNGTSFWIDRMLNQTGNNPDTNDLFSRGR
                     MLLLAGHNPSVIGFAGEARYGDRVNDPGKNIYTVALSPGTFTENVTLRNNCPSYFATE
                     FNSGNINVKETKFITDNNVAVTLLTFKNNGSGTQSITITANSPYAATASGSELVGNVA
                     APLLWTGFNANYIPGVTNCYVRMSGDSMTVNGGNLVRTISIAAGQTIQTKVLLGWTTS
                     ELPESTTEYNTFKSYANDTAFAKQVQTYNKWWADNIPYFDVPDSYIKKVLYYRWWINR
                     FDSIDGNIPGNDWQFPAPVEGALGYANQITVSMPWIMDDIKYLRNPLYPYGTFLTAGE
                     ETEVGAYRDNPARGWMWNKVMMQYISKAGWEGYKVHGGPLTMLKNFARYAENDINGQL
                     SYYDQNNNKLLAYSDSTLTGNDGCLPSMQYYSTNGYERNSERIDASAYHYGNTVAAAQ
                     MYSYLGNSAKASAMNTLASTIQTNFMNTFWDEGTRQFLHYDTASGAREYWKDNNFNGY
                     WMGIIPTTDPKYREMFNLFNNNGDYFTFPFFTGDYKDIDARYNKLKATGQFNTWYANT
                     FGYCNGGVNLSILAAAVKKYPNSYVTADTFKKTLYWDAWLHCIQDGNANYLDANEFYW
                     LENTNNDTKVTSTMQQTGRIVRSWIHHNQLAKMNSNVIEDVMGFTPREDNKIELWPID
                     IGWDHFTVNSLRYHGSDLTIIWDKPGDGKVYYGNVPEGYSLYINGARMFTINGLSHAI
                     WDSQTGSVTLPEGGAGVSYNVANTAFKEAKAVTYGDSRLLDIFQKAGRDISGTTE"
     gene            complement(3139..5112)
                     /locus_tag="VF531_12590"
     CDS             complement(3139..5112)
                     /locus_tag="VF531_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX7714844.1"
                     /translation="MKDATTFLPWKRLRSLPLKQVKIEDVFWAGHQRLIREVVIPYQW
                     DALNDRIPDAAPSHCIRNFRIAAGLERGEFQGVIFQDSDLAKWLEAVAYNLETHPDCG
                     LEQTADEVTVLIERAQQPDGYLNTYYTLKEPGKRWTNFREGHELYCAGHLIEAAVAYY
                     NATGKRRLLDVVCRFADHIDTVIGTKPGKLKAYPGHQEIELALVKLFRVTGNVRYLDL
                     GKYFIDERGREPYYFDIETGKRGNTFYFPEFEKFDRTYAQTHLPVRQQSTAEGHAVRA
                     VYMYSAMADLAVEAGDQQLFEACRKIWENIVTRRMYITGGIGAAAFGEAFTFDYDLPN
                     DTMYTETCASVGLFFFAQRMLQLELNHCYADVMEKTLYNTILAGMALDGKHFFYVNPL
                     EVWPEACEKNPAKNHVKVSRQKWFGVACCPPNIARLLSSLGRYIYSQAEDEIFIHLYI
                     GNRAAFEIRGEKIQLIQETNYPWDGRVRLSVTTAGPCEMALTFRIPGWCRQATLRVNG
                     MEIDDATPKSGGYITLRRTWNPGDYIELEFSMPVVLMRSDPNVRADIGKLAIQRGPMV
                     YCLEEADNGKDLHRISIRPDEVFAAEFDPDLLGGVVVISGEAERVADSTWGCELYRIA
                     DKDRRDKVMIKAIPYCFWNNREPGEMLVWIREG"
     gene            complement(5143..5985)
                     /locus_tag="VF531_12595"
     CDS             complement(5143..5985)
                     /locus_tag="VF531_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714845.1"
                     /translation="MTGRNQRANLFLIVLTFVLAAVFMIPLYWMVRSSFMNLKELYQM
                     DPMLLFPPKLRLDNYLRVFMDSNINLLQELKNSLVIAIAVVFGTVITSSLAAFGFARL
                     RFPLRNLWFALVISSMLLPSAVTLIPTFLMWNALGQIGSFYPLIVPAWLGGGAYFIFL
                     LRQFFLTIPRDLDEAAIIDGANYFTVYYRVMLPLIKPALVVVALFSFIGAWNEFFYAY
                     IYLSAEESYTLMLGLQTLRGVQLADFTGVMAMATLVTIPTFIFFLIGNRYFVEGITLT
                     GIKG"
     gene            complement(5985..>6440)
                     /locus_tag="VF531_12600"
     CDS             complement(5985..>6440)
                     /locus_tag="VF531_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714846.1"
                     /translation="QRNFLGDEALVIPALVIMSLWFCGGTIVVFLASLQDVPVELREA
                     VAIDGGNKWHEFIHVTLPCISPVIFFQFIIGLVGAVQIFTQVVVMTNGGPNNVTRFLN
                     FLIFEDAFRHFRMGYASAEAFTAFVIVLILTALIFKSSNFWVHYQGGDK"
BASE COUNT         1668 a         1663 c         1490 g         1619 t
ORIGIN      
        1 ttcggtggtt ccggagatat cccttcctgc cttctggaag atgtcaagca gtctgctgtc
       61 gccataggtt accgcctttg cttctttaaa cgccgtgttg gcgacattat aggaaacgcc
      121 ggctccgcct tcgggtaagg ttacgctacc ggtctgcgag tcccaaatcg catgactcaa
      181 cccattgatt gtaaacatcc gggcaccatt gatatatagc gaataaccct cgggaacatt
      241 accgtaataa accttgccat cacccggctt atcccagatg atggtcaggt cgctgccatg
      301 gtacctcagg ctgttgacgg taaaatgatc ccagccgata tcgatcggcc agagctcgat
      361 tttgttgtct tcacgcgggg taaagcccat cacatcttca ataacattcg aattcatctt
      421 ggcaagctga ttatggtgaa tccaggacct gacgattctc ccggtctgtt gcatggtaga
      481 agttactttc gtatcattgt tggtattttc cagccaatag aactcattgg catcaagata
      541 attggcgttg ccgtcctgaa tgcaatggag ccaggcatcc cagtagagcg ttttcttaaa
      601 ggtatcggcg gttacatagg agttcgggta tttcttgacc gcggccgcca gtatcgaaag
      661 gttgactcct ccattgcaat acccgaacgt attggcatac caggtattaa actgaccggt
      721 tgctttcaac ttattatacc tggcgtcgat atctttgtag tcgccggtaa aaaacgggaa
      781 agtgaagtaa tccccattgt tgttgaagag gttgaacatt tcccggtact tcggatcagt
      841 ggtcgggatg atgcccatcc aatagccgtt aaagttgttg tctttccaat attccctggc
      901 gccggaggct gtatcataat gcaggaactg gcgggttcct tcatcccaaa atgtattcat
      961 gaaattggtc tggatcgtac tggccagcgt attcatggcg gatgctttcg cggaattacc
     1021 caagtagctg tacatttgcg ctgcagcaac cgtattgccg taatggtatg ccgacgcatc
     1081 gatccgttcg ctgtttcgtt catagccatt ggtactataa tattgcatcg acggtaagca
     1141 gccgtcgtta cctgttaagg tactgtccga gtacgctaag agcttgttat tgttctggtc
     1201 atagtaagaa agctgcccgt tgatatcatt ctcagcatac ctggcaaagt tcttaagcat
     1261 ggtgagcgga ccgccgtgaa ccttgtaccc ttcccagcca gctttggaaa tatactgcat
     1321 catcaccttg ttccacatcc aaccccgggc ggggttatcc cggtatgccc caacttccgt
     1381 ttcttcacct gcggtaagga aggtgccata aggatagagc ggattccgca agtacttaat
     1441 atcatccatg atccacggca tactcaccgt aatctggttg gcataaccaa gcgcgccttc
     1501 taccggcgcc gggaactgcc agtcatttcc ggggatattg ccgtcgattg aatcgaagcg
     1561 gttgatccac caccggtagt agagcacctt cttgatatat gaatcaggca cgtcgaaata
     1621 tggaatatta tccgcccacc acttgttata ggtctgtacc tgtttggcaa aggcggtatc
     1681 gttggcataa cttttgaagg tattatattc cgtagtggat tccggcaact ccgacgtggt
     1741 ccaccccagc agcaccttgg tctgaattgt ctgtccggct gcaattgaaa tggtccttac
     1801 cagattaccg ccgttcaccg tcatactgtc gccactcatc cggacgtaac agttggtaac
     1861 accgggtatg taattggcgt taaaacccgt ccataaaagc ggtgccgcga cattacccac
     1921 caactcggaa ccgcttgccg tagcggcata gggcgaattg gcggttatcg tgatactttg
     1981 tgtgcccgat ccgttattct tgaatgtaag caatgtaaca gccacattgt tatcggtaat
     2041 gaatttggtt tctttgacgt tgatgttgcc gctgttgaat tcggtggcaa aataactcgg
     2101 gcaattattc ctgagcgtca cattctcggt aaaggttccg ggcgacaggg caaccgtata
     2161 aatattcttt ccggggtcat tcaccctgtc tccataacgg gcttcaccgg caaagcctat
     2221 gacggacgga ttatgtcctg ccagtaacaa catccggcct ctgctgaaga gatcattagt
     2281 atcggggtta ttgcctgttt gattcagcat ccggtcgatc cagaatgatg tgccgttctt
     2341 ttccgcatca taaattgtct gcatcatgcc gttaacaata taagcaaccg gttcggcggg
     2401 ggccgccgca cccgagaagg tcggccaacc cggatcccgg ggtgaggtaa aattatactg
     2461 catcgtgtag gcaaataaaa cctgggtgcc ggctaacagg aatatggcac cgccgagaat
     2521 aaccgactta agctttttgg atctttgaat gctcttcatg tacttacctc ctttttttca
     2581 ttgatcattt ccaccgactc taaaaattcc gatctgttct tccatttcca cctcccttca
     2641 gaccgcaagc aacttcccga ctgtgcaatc cgcagagaaa aaacgcgccg ccctaacccg
     2701 aactccactg tgggataatg aatccgggta cggtaaggtc gaaaatgttt cgaatgtttc
     2761 gtaacccgaa gttgcatcct gcaggcgaaa atgccttgaa atgcaatttg gtacaaatat
     2821 ggcgcgaaat aggattgatt ggaatcatga tacttaggca ggataatcag gtgaaaataa
     2881 ctttgcccct ggggttgata ctgcacgtgt ttgtacacgc gagggctcgt atattttcat
     2941 cacttttctg aaacccaatt cttcagcttt catcatgtag gaaggaaaaa tcggttttta
     3001 tctcggaggc acactcatcg ccgaaataga tcggagttat cggagacggc gcatgatacc
     3061 caatgctggc accagtagat aatgaaaaaa tattgatagg acctaaactc acctctggtc
     3121 tcagagcaaa atccggcctt atccctcccg gatccatacc agcatctcgc cgggctcgcg
     3181 attattccag aaacaatatg gaattgcctt tatcattact ttatcccttc ggtctttatc
     3241 cgcaatccga tacagctcac aaccccaggt tgaatcggcg acacgttccg cttcaccgct
     3301 gatcacaacc acacctccca gcagatcggg atcgaattcc gccgcaaaca cttcatccgg
     3361 tcgaatcgat attctgtgaa gatctttccc gttatccgcc tcttccagac aatacaccat
     3421 cggtccgcgc tggatggcga gtttgccgat atcggcccgg acattgggat cagatctcat
     3481 cagcaccacc ggcattgaaa actcaagttc gatatagtcc cccggattcc aggttctgcg
     3541 gagcgtaata tagccgccgg acttcggggt ggcatcatcg atctccatac cgttcactct
     3601 cagggttgcc tgccggcacc aaccgggaat ccgaaaagtc agcgccatct cacaaggccc
     3661 ggcagtcgtt accgataatc gtacccggcc atcccaaggg taatttgttt cctggatcag
     3721 ctgtattttt tcaccccgga tctcaaaagc agcccggttg ccgatatata agtgaataaa
     3781 aatttcatct tcggcttgac tgtaaatgta acggcccaac gagctcaata atctggcgat
     3841 attcggcggg cagcaggcaa caccgaacca tttttgccgg gagactttca catggttttt
     3901 ggctggattt ttttcacagg cttccggcca tacctcgagg gggtttacat agaaaaaatg
     3961 tttgccgtca agcgccatgc cggccaatat cgtattataa agcgtcttct ccataacatc
     4021 cgcataacaa tgattgagct ccaactggag catccgctgg gcaaaaaaga ataatccaac
     4081 cgaggcgcag gtttcggtgt acatggtatc attgggaaga tcataatcga aagtaaaggc
     4141 ctctccgaat gccgccgcac caataccacc ggtgatatac attcttctgg tgacgatgtt
     4201 ttcccaaatc ttgcggcaag cctcaaaaag ctgttgatca ccagcttcaa cggcaagatc
     4261 cgccatggcc gagtacatat agaccgcccg taccgcatgt ccttccgccg ttgactgctg
     4321 ccggaccggc aggtgggttt gggcatatgt gcgatcgaac ttttcaaact cgggaaaata
     4381 aaaggtattg cctcgtttgc cggtctcgat atcaaagtaa tatggttccc ggccgcgttc
     4441 atcaatgaag tatttaccca aatcaagata tctgacattg cccgtcacgc ggaataactt
     4501 caccaatgcc agctcaatct cttggtgacc gggataggcc tttagcttcc cgggtttagt
     4561 cccgatcact gtatcaatat gatccgcgaa ccggcacacc acgtcaagca atcttctttt
     4621 cccggtagca ttgtaatatg cgactgccgc ctcgatcaaa tgcccggcgc aatagagctc
     4681 gtggccttca cgaaagttag tccagcgttt tcccggttct tttaaagtgt aataagtatt
     4741 caagtatcca tccggttgct gggccctttc aataagcacg gtgacttcat cggctgtctg
     4801 ttccaatccg cagtccggat gagtttccag gttgtaggcc accgcctcca gccattttgc
     4861 caaatcactg tcctggaaga tcactccctg gaattcaccc cgctcgagtc cagccgcgat
     4921 ccggaaatta cggatgcaat ggctgggggc ggcatcggga atccggtcgt ttaatgcatc
     4981 ccactggtag gggataacca cctcccgtat aaggcgttga tgtccagccc aaaaaacgtc
     5041 ctcaatcttg acctgtttca gcggcaaaga tctgagccgt ttccacggca agaatgtcgt
     5101 tgcgtccttc atgattggtt ctccttaatc ttgcaaatta aatcaacctt tgatacccgt
     5161 cagggtaatc ccctccacaa aatatctgtt cccgatgagg aagaaaataa atgtcggtat
     5221 ggttaccaaa gtagccatgg ccatcacccc ggtaaaatcg gccagttgaa cgccgcgcaa
     5281 agtttgcagc ccaagcatga gtgtatagct ctcctcggcc gacaaataga tataggcata
     5341 aaagaattca ttccaagccc cgataaaact aaaaagcgcc accactacca aagccggttt
     5401 gatgagcgga agcatgaccc ggtagtatac ggtaaaatag ttggcgccgt caataatcgc
     5461 cgcctcatcc aagtccctgg gaatggtcag gaaaaattgg cgtaacaaaa agatgaaata
     5521 ggcccctccc cccagccatg cgggaacgat taacggataa aaactgccga tctggcctaa
     5581 agcgttccac atcaaaaaag tgggaatgag ggtaaccgcc gatgggagca acatcgaact
     5641 gattaccagg gcaaaccaga gatttcgcaa gggaaacctt aggcgggcaa aaccgaaagc
     5701 cgctagacta ctggtgatga ccgtgccgaa taccacagcg atcgctatca caagcgaatt
     5761 cttcaattcc tgcaatagat tgatattgga atccataaaa acccgcaaat aattatccag
     5821 tctcagcttc ggcgggaata gcagcatcgg atccatttgg taaagttcct tgagattcat
     5881 aaacgaactg cggaccatcc agtacagggg gatcataaaa acggccgcca aaacaaaggt
     5941 taagacaatc aggaacaggt tggccctttg attcctgccc gtcattattt gtcaccccct
     6001 tgataatgaa cccagaaatt cgatgattta aagatgaggg cggttaagat caaaacgatc
     6061 acgaaagcgg taaaggcctc ggctgatgca taccccattc ggaaatggcg gaaagcatct
     6121 tcaaagatta ggaagtttaa aaaacgcgtt acattgttag gaccgccgtt ggtcatcacc
     6181 acgacctggg taaaaatttg aaccgcgccg accagaccga tgataaactg aaaaaagatg
     6241 accggagaga tgcacggtaa ggtaacatgg atgaactcgt gccatttatt gccgccgtca
     6301 atggcgacgg cttcacgtag ctctaccgga acatcctgca aggaggctaa aaaaaccacg
     6361 atggtccctc cgcaaaacca gagactcatg atgacgagtg ccgggattac cagcgcttca
     6421 tcgcctagaa aatttctttg
//
LOCUS       DASVMB010000451         7547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_277949,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000451 DASVMB010000000
VERSION     DASVMB010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277949"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VF531_12605"
     CDS             complement(<1..834)
                     /locus_tag="VF531_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7714847.1"
                     /translation="MRVIPIEELEPGMILAKPILRERDGAILLQDRIELKPSYIERIK
                     SLNYTSVCILDPETPEEIETLTPIREETRFKATALLRTTFSQFKENDRMDTAKLTALV
                     HEIIDQILSDPRTVYNLSQIRAFDNYTFTHSVDVAVLSVLIGTTIALNRNDLEILGIG
                     AMLHDIGKVFTDLRILNKPSQLNPEEYELIKLHTRMGYESLRTRTNISFLVPHMTLQH
                     HEREDGSGYPRGLVGTRIHRFAKIIAVADVFDAMTSYRLYQEPVPSQIAIQEICEKSG
                     TK"
     gene            1156..2202
                     /locus_tag="VF531_12610"
     CDS             1156..2202
                     /locus_tag="VF531_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714848.1"
                     /translation="MKTKAWAWLVVVLLVSLFVGPLYAAASHKIALKLRPFPITDSRF
                     YIQGVIDGRSDRSDLGEIQIWSHYQEPLILEGDLRTDLQALADISFKKTTDKIPVVMK
                     VNLLRFSSWSGLNRYIKVEAQIAFYRVQDGKLGKVYQTEAYLEQAESGLDQYERMLRS
                     VIGKCLTGFAATSWESVAPEWQDYQTVVAQTTPSDATLSLTMPERHRIYLLESISVPG
                     GNGGGLLRYDQDVNQKDWAWVDNSATEIFSAKNGTNSGMVYNLESRSGVMRRLGDSGF
                     AILVEWGMPFGVESLPDYSRIIYGLNLQESLVYFPLEKHGLAATASFYQIRLFNSDIY
                     PQDSGFRIGIGYQF"
     gene            complement(2276..3508)
                     /locus_tag="VF531_12615"
     CDS             complement(2276..3508)
                     /locus_tag="VF531_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714849.1"
                     /translation="MRTRENLRNSFKSLSHNPLRTFLTMLGVIIGVLSVIILTSIGEG
                     VKDQVTAEVEALGANLLYIIPGRISFTPGPRQSKLGISVNPFGSGRSSLTYDDVLALK
                     KQKQIAAVTGIYNGIDRLDILNLMVATTGVDEDMAKIRGLNLKYGRFFTAQERKSRAR
                     VSVIGDQANREIFSGRNSIGQSFKLNGKDYRVIGVLQYKKPENFGPGSEDLNIKIYLP
                     ITEIISRNSLPRLQQIIVKARTTGQVRPVETIIRRILKNRHQTEDFSVFKQQDMLNAI
                     NNILSILTAALGGIAAISLVVGGIGIMNIMLVSVAERTREIGVRKAIGAKYRDILVQF
                     LIEAVTLSLLGGLLGLALGVAGSRMIPLLIPTIHTAVSPLAVAISLLFAVLVGVFFGV
                     YPAALAAKLDPIEALRSE"
     gene            complement(3505..4182)
                     /locus_tag="VF531_12620"
     CDS             complement(3505..4182)
                     /locus_tag="VF531_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714850.1"
                     /translation="MGIIIHTLYKSYRNGTEITEVLNNVSLSVADGEFAAIVGPSGSG
                     KSTLMNIIGCLDRFNYGSYRLNGLDIGGLSQNQLAEIRNRHIGFIFQFFNLLPQYTAL
                     ENVELPLFYGDISPKIAREKAISALTMLGLKNRMHYRPNQLSGGQKQRVAIARAIINS
                     PSIILADEPTGSLDTRTGREVMDILKDLNNQKKTVIMVTHDPNLAGLADRIIQIRDGI
                     IVDGSVS"
     gene            4446..6707
                     /locus_tag="VF531_12625"
     CDS             4446..6707
                     /locus_tag="VF531_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX7714851.1"
                     /translation="MYQFDLNGAWKMKRTLDSDWSDVTVPGSVFHDLLNAGKIEDPFY
                     RANEEKALEIAEHDYEYQREFTPDPQILQCERVFLSCEGLDTLAGIDINGFPLAKTDN
                     MHRSYEWEIKSLLHPGTNIIHITFLSPLPYIRQKQDEHPTWGAGDAVTGFQHLTVTVK
                     IDPPQGPVLTQSITGLEPQKNLRFDIDQPDLWWPNGYGEHPLYRVEVLLTQDNTLLDR
                     NEFRIGLRRLTIKQEKDEWGESFQFEVNGLSIFTMGADYIPEDNLLPRCNPERTERLI
                     RDCVDAHFNCLRVWGGGLYPPDYFYDLCDRYGLIVWQDFMYACASYVLTEEFAANVRQ
                     EAIDNIRRIRHHPCLGLWCGNNEMEEGWASWNFTKTPRLQTDYIKQFEILLPDLVNQY
                     DPNTFYWPSSPSSGGGFENPNSEKIGDVHYWDVWHGLKPFTDYRNHYFRFVSEFGFQA
                     FPGPKTVAVFTIPEDRNIFSYVMERHQKNASANGKILYYLSQNLKYPKDFEALLYASQ
                     VLQAEAIQCGVEHWRRNRGRCMGAIYWQLNDCWPVASWASIDYFGRWKALHYFAKRFF
                     APVLLSACETDTQVGLYLSNDRATPFIGEILWRLRKSDSRIIREGRIPAQAAPLTAAL
                     CQELQFNGEPGFERRDTYLEYSLIEAGQTLSLGSVLFAPEKHFEFLDPRIEYTLNELE
                     DRYIVSLTSRAFARCVALDLAKADCQFSDNYFDLSGSEVKEVAILKESLSAALSLAEL
                     KGELCIRSLYDIV"
     gene            6927..7382
                     /locus_tag="VF531_12630"
     CDS             6927..7382
                     /locus_tag="VF531_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7714852.1"
                     /translation="MNRSLLSTRTVTQVGILVHDIQKTAQKYADFLGVDLPNIIETDV
                     YEKARTEYQGTPSKAQAKLAFFNIGSNLQLELIEPDHEPSTWRQYLDEHGEGMHHLAL
                     VIQGMSEKTAILAENGLPLIQKGEYQGGRYAYIDATRDLKVILELLEND"
BASE COUNT         1792 a         1836 c         2010 g         1909 t
ORIGIN      
        1 tttcgtcccg gatttttcgc aaatctcctg gatggcgatt tgggagggta ccggttcctg
       61 ataaagacgg taagaggtca tggcatcaaa gacgtcggcg actgcgatga tcttggcgaa
      121 acggtgaatc cgcgttccga caaggccccg gggataaccg gaaccgtctt ctctttcgtg
      181 gtgctggagt gtcatgtggg gtactagaaa actgatattg gtccgagtgc gcagcgattc
      241 ataccccatc cgggtatgca gcttgatgag ctcatattcc tcgggattta gctgggaggg
      301 tttattgagt atcctgagat cggtgaagac ctttccgata tcatgaagca ttgccccgat
      361 gcccagaatc tccaaatcgt tccggttgag ggcgatggtt gtcccgatga gtacggagag
      421 aaccgccaca tccaccgaat gggtgaaggt ataattgtcg aaggcgcgga tttgggaaag
      481 attatagact gttcggggat cggacaggat ttggtcgatg atctcgtgaa ccagcgcggt
      541 caacttcgcc gtatccatcc ggtcgttctc tttgaactgt gaaaaggtgg ttcttagcaa
      601 ggcggtcgcc ttgaaccggg tctcctcgcg aattggcgtc aaggtctcga tctcttcggg
      661 agtttccgga tcaagaatac agacggatgt ataattgaga gactttattc tttcgatata
      721 ggagggtttt aattcgatcc tgtcctgtaa aaggattgct ccgtctcttt ccctgagtat
      781 cggtttagcc agaatcattc ccggttccaa ctcttcgatc ggaataactc tcatagacca
      841 taccccctat attacacaca atttcaccaa cttttaatta taacagaatt taattcaagg
      901 ttgtgtaaaa ttttgggggt aatttccttt aaaatccggc agatcccgca agatacaggg
      961 tctcctcatg cattagcccg tttgggctga acagggagag ggttatgata aggtataata
     1021 ggttccaatt tggtgaactt tcatctccgg cgcaggaaac tcttttgcgg gattgaatag
     1081 attactcgag ctttgtaatt tgcagaagat tatttctgac acgcgtgtgc acagaatact
     1141 tcaggaggat acggaatgaa aacgaaagcc tgggcctggt tggtcgtagt tttgctggtc
     1201 tccttatttg tcgggccgct ctacgctgcc gccagtcata agattgcgct taaactccgc
     1261 ccgtttccta tcaccgatag ccggttttac atccagggtg tgatcgacgg ccgcagcgac
     1321 cggagcgatc tgggggagat tcaaatctgg tcccattacc aggaaccact gattctggag
     1381 ggggatcttc gcaccgacct acaggctctt gccgatatca gttttaagaa gacgacggat
     1441 aaaataccgg tcgtgatgaa ggttaatctg ttgcgcttta gttcttggag cggattaaac
     1501 cgctatatca aggttgaagc acagatcgct ttttatcggg ttcaggacgg gaagctgggg
     1561 aaggtttatc agaccgaagc atacctggag caagcggaat ccggattaga tcaatatgaa
     1621 cggatgcttc gttcggtgat tggaaaatgc ctcaccggtt tcgccgccac cagctgggaa
     1681 tcggttgccc cggaatggca ggattaccaa acagtcgtcg cccagactac cccgtcggat
     1741 gcgaccctct ccctgactat gccggagcgt caccgaatct atcttctgga atcgatctcc
     1801 gttcccggag gtaacggcgg gggcttgctc cgctatgatc aggacgtcaa tcaaaaggat
     1861 tgggcctggg tggacaactc cgccactgaa attttttccg ccaagaacgg taccaacagc
     1921 ggaatggttt ataacctgga aagccgttcc ggagtgatgc ggcgcttggg agactcggga
     1981 ttcgccattc tggtggaatg gggtatgcca tttggagttg aatccctgcc ggattactca
     2041 cggattatat acggcttgaa ccttcaggag agtttggttt atttccccct ggagaagcat
     2101 ggtctggctg cgacagccag tttctatcag atccggctgt tcaattcgga tatttaccct
     2161 caggatagtg gttttcggat cggaatcggg taccaatttt aaagactgcg aaaaaaggtc
     2221 agagcaaaga gggcgcaagc cctctttatt tttaatacgc cccggcctac tcccttcatt
     2281 cactccgcaa tgcctcgatc ggatcgagtt tggcggcaag ggctgccgga taaactccga
     2341 aaaatacccc caccagtact gcaaagagca aagatatcgc cactgccagc ggagaaaccg
     2401 cggtatggat tgtcggaatc aataacggaa tcatccggga tcccgccaca ccgagagcca
     2461 gtcccagtaa tccaccgagt aaactgagag taaccgcttc aatcagaaat tgaactaaaa
     2521 tatcgcgata tttggcgcca attgccttcc ggaccccaat ttccctagta cgttccgcca
     2581 cggaaaccag catgatattc ataatcccga ttccccccac taccagggat atggcagcga
     2641 tccctcccag tgctgcggta agaatggata gaatattgtt gatcgcgttc agcatgtctt
     2701 gttgtttgaa caccgaaaag tcctcggttt gatggcgatt tttaagaatg cggcggatga
     2761 ttgtttcaac cggccggact tgtccggttg ttcgggcttt tacgatgatc tgttgcaggc
     2821 gggggaggct gtttcgggaa atgatttcgg tgatcggaag gtagattttt atattcaaat
     2881 cttcactacc ggggccgaaa ttttccggct ttttatactg caatactccg attacccggt
     2941 agtcttttcc attcaattta aagctttggc cgattgagtt ccggccggag aagatctcgc
     3001 ggttggcttg atcaccgatg actgataccc tggcccggga tttccgttct tgggcggtaa
     3061 aaaaccgtcc gtatttcaga tttagccccc ggatcttcgc catgtcctca tcgacaccgg
     3121 tggtggccac catcaaattc aagatatcca gacggtcgat tccattataa atcccagtaa
     3181 ctgcagcgat ctgtttttgt tttttcaagg ccaatacatc atcatacgtc agactgctcc
     3241 ggccggatcc gaacggattg acggatatcc ccagcttact ttggcgaggc ccgggagtga
     3301 atgagattcg gcctggaata atatataata aattggctcc cagagcctca acttccgcgg
     3361 tgacctgatc ttttacgcct tcaccgatgg aagtcagtat aatcacggat aggactccaa
     3421 tgattacccc gagcatggtc agaaaggtcc gtagcgggtt gtgcgacaag ctcttgaagc
     3481 tgttacgcag gttttcacgg gtcctcatga gacgcttccg tctacgatga ttccgtcgcg
     3541 gatctggata atcctgtcgg caagcccggc cagatttgga tcatgggtca ccataatcac
     3601 cgtctttttt tggttgttga gatcttttaa gatatccatc acttcccggc ctgtccgggt
     3661 atccaggctt ccggtaggtt catccgccag gataatactg ggggaattga tgatggcgcg
     3721 ggctatggct acccgttgtt tttggcctcc ggagagctga ttgggccggt aatgcatccg
     3781 gtttttgagg cccagcattg tcagggctga gatcgctttt tcacgggcga ttttaggaga
     3841 aatatcgccg taaaacaggg gcaactccac gttctccaaa gcagtgtact gagggagcag
     3901 gttaaaaaat tgaaagataa agccgatgtg gcgatttcta atctccgcca gctggttttg
     3961 ggataagccg ccaatatcca agccgttcaa gcggtaggaa ccgtagttga aacggtccag
     4021 acaaccgatg atgttcatca gggttgattt ccctgagccg gacggcccga cgattgccgc
     4081 gaattcaccg tcagctaccg agagtgagac gttattaagt acttcggtga tttcagtacc
     4141 gttgcggtaa cttttgtaaa gcgtatggat aatgattccc aaggtgtttt ggatgctcct
     4201 tgtggtttgg ccgatggaca gtatgattat ggcgaaggga actgttaatt atacgcgttt
     4261 tttaagtttt ggcgtttggg agcagctgtg ttttttacct aagttcttgg cataaaaaaa
     4321 tcccaatacc gacaggaaaa aacggccttt tgaagaaata tcgtatgtcc atccggagga
     4381 acgattgatc cggttggaac aaactatatc tgccacggtt gttgaaggaa tttggagggc
     4441 tgccgatgta tcaattcgac ctgaacggcg cttggaaaat gaaacggacg ctcgattccg
     4501 attggagtga cgtaacggtt ccgggttcgg tgtttcatga tctcctgaac gccggaaaaa
     4561 tcgaggatcc tttttaccgc gccaatgagg aaaaggcgct tgagattgcg gagcatgatt
     4621 acgaatatca acgggaattc actcccgacc cccaaattct ccagtgtgag cgagtgtttt
     4681 taagttgcga aggtttggac accctggcgg ggattgatat taacggtttt ccattggcta
     4741 aaaccgataa tatgcaccgg agctacgagt gggaaatcaa atccctgctc catccgggaa
     4801 cgaatatcat ccatattaca tttctttcac cgctgccata tatccggcaa aagcaggacg
     4861 aacatcccac ctggggagcg ggtgacgcgg tcaccgggtt tcaacatctg acggttacgg
     4921 taaagattga tccgccgcaa ggtccggtgc ttacccaaag cattaccggc cttgaaccac
     4981 agaagaacct gagatttgat atcgaccaac ccgacctatg gtggcccaac ggttatggtg
     5041 agcatccgct ctaccgggtg gaggttttat tgacacagga taacaccttg cttgaccgga
     5101 atgagttccg gatcgggcta cggcgtttga caattaaaca ggagaaggac gagtggggag
     5161 aatccttcca gtttgaggtg aacggccttt cgatcttcac gatgggtgcg gattatattc
     5221 ccgaggataa tctgctcccc cgctgcaacc cggagcggac cgaacggctg atccgggatt
     5281 gtgtggacgc gcatttcaat tgtttgcggg tttggggagg cggactttac ccgcccgatt
     5341 acttttacga tctctgcgac cgttatggcc tcatcgtctg gcaggacttc atgtatgcct
     5401 gcgccagcta tgtattgacc gaggagtttg cggccaatgt cagacaagag gcgattgaca
     5461 atatccggcg gattcggcac cacccctgcc tgggactatg gtgcggcaat aacgagatgg
     5521 aagagggctg ggcctcttgg aatttcacga aaacgcccag actccaaacc gactatatca
     5581 agcaatttga gattctcctt ccggacttgg tgaaccagta tgatccgaat actttttact
     5641 ggccttcttc gccgtcctcg ggaggggggt tcgagaatcc caacagtgaa aagatcggtg
     5701 atgttcatta ttgggatgtc tggcatggtt taaagccgtt caccgattac cggaaccatt
     5761 actttcgatt tgtttcggag ttcggttttc aagccttccc cggcccgaaa accgtggccg
     5821 tttttaccat tcctgaagat cgcaatatct tttcctatgt gatggagcgg caccaaaaga
     5881 acgcctccgc caacggcaag attttatatt atctctctca gaacctgaag tatcccaagg
     5941 atttcgaagc cctgctctac gcctcccagg tgttgcaggc cgaagcgatt caatgcgggg
     6001 tggaacattg gcggcgtaat cgtggccgat gcatgggggc gatctactgg cagctcaacg
     6061 attgctggcc ggtggcttcc tgggcgagca tcgattattt cggccgttgg aaggcgctac
     6121 actacttcgc caaacggttc ttcgcaccgg tgctgctatc cgcctgtgaa accgataccc
     6181 aagttgggct ctatctcagc aacgaccggg ccacgccgtt tataggagag attctctgga
     6241 ggttgcggaa gagcgattcc cggatcatcc gggagggcag gatcccggct caagcggctc
     6301 cgcttacggc agccctctgt caggagctgc agtttaacgg tgaaccgggc tttgaacgtc
     6361 gtgatactta tctcgaatac agcttgattg aagcgggtca aacgttgagt ttggggagcg
     6421 tactctttgc cccggagaaa cattttgagt ttttggatcc ccggatcgaa tataccctaa
     6481 acgaactgga ggatcgctac atcgtctccc tgacttcccg ggctttcgcc agatgcgtcg
     6541 cattggattt ggcaaaagcg gactgccaat tcagcgataa ttactttgat ctctccggaa
     6601 gcgaggttaa ggaagtggca attctcaagg agagtctttc cgcagcacta tctcttgcgg
     6661 agctaaaagg cgaattatgt atcagaagcc tgtacgacat cgtctagggg accccgcgcg
     6721 cccgttgggc gcgcgacacc cctcggtcga cgtccgggtc ggccctcccc catttagcat
     6781 ctattcattt gaagtccatt cgagatgggg gagggaacca tgctgtagag ggggaggcgc
     6841 gagacacctt gaaacagcct aagggatgag aaccacgacg gtagtagggg aacgtcgtca
     6901 caaatatcaa tgtgaggtgg tttgttatga atcgtagctt gctaagcacc cggaccgtga
     6961 ctcaggtggg catcttggtc catgatattc aaaaaaccgc ccaaaagtat gccgattttt
     7021 tgggagtgga cttgcccaat atcattgaga ccgatgtata cgagaaagcc cgtaccgagt
     7081 atcagggaac ccccagtaaa gcccaggcca agctggcatt cttcaatatc ggttccaatc
     7141 tgcaattgga gctgatagag ccggatcatg aacccagcac ctggcggcag tatctcgatg
     7201 aacacggaga gggcatgcac catctggccc tggtcatcca ggggatgtcc gagaaaaccg
     7261 ccattttggc cgaaaacggg ttgcccctga tccaaaaggg tgaataccaa ggcggccgct
     7321 acgcctatat cgatgcgacc cgggacctca aggtcattct ggaacttctg gagaacgact
     7381 gagttccggg actgtcagag ccaggagaga atctcccgca ttccccgggc cggatcctcc
     7441 aggggataat actcaagccc catatatcct tgatatccgg ttcgagcgat cgcccgtaat
     7501 acctccgggt aattgatttc accgtgggtc agttcatggc ggcccgg
//
LOCUS       DASVMB010000452         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_424897,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000452 DASVMB010000000
VERSION     DASVMB010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424897"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            868..1668
                     /locus_tag="VF531_12635"
     CDS             868..1668
                     /locus_tag="VF531_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7714853.1"
                     /translation="MAVSKKPTWTGSLNRLGRGLFVLLFNMGEIAQLYLKSFRSLFRR
                     TLFWENTITQMESIGVRSLPLVLIISASTGMVFSLQIAKIFATFGLTSQLGQGLTLAF
                     ARELGPVLSAVIVAGRVGSAIAAEIGSMKVTEQIEALETLSTDPIDYLVAPRIIAAGL
                     MLPILVIFADFIGIIGGIAVAVSYANIAPTDFIDGILTWVTFWDFAGGIIKAVFFGLV
                     IAGIGTYWGLRTENGAVGVGKATTESVVTASIIIFVLNYFLSILLYNL"
     gene            1775..2509
                     /locus_tag="VF531_12640"
     CDS             1775..2509
                     /locus_tag="VF531_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016476596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7714854.1"
                     /translation="MNIISLYNIGYRVGGQTILDGLDLEFEKGESFVIMGRSGCGKST
                     LLRLIIGLIRPTSGWIEVDGRNTLNLSPQEWQAVRYQMGMVFQSAALFDSMTIYDNVA
                     FGLRRKNLRDSEIRERVFEALKIVGLEPATALKMPSDLSGGMKKRTAIARAIAFKPPI
                     LLYDEPTTGLDPIMSDTINELIRDLQRNLEVTSIVVPHDIMSALKVADRIGLLSEGRL
                     VELATRSELDRDRHPKLAQFMKGYSF"
     gene            2544..4004
                     /locus_tag="VF531_12645"
     CDS             2544..4004
                     /locus_tag="VF531_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7714855.1"
                     /translation="MQWNNPETKVGFFTLLALLAMFFLFLWLNGSQLFERGHEVEAIF
                     TRIEGVRPGAPVKFSGVDIGRVTRIYFEDFNVVVVMRIRSDFKVPRAAKAVIASSGVV
                     GDMFIEIMPYRVGETIVPMRGNRLTGQNPIAMDQFYNTAYEVIASLQQITDSIRGLTE
                     NPEVIESLRQSLVRLDTITKDISHISGQLEQLDYPGLFRRMENTMLIVERLAAANEPR
                     INTLIENISLASVQLTQASITANRFLKDIDADGETAADLQQTLAHAEQVAADLEKLSS
                     YLASHTGEIDQLFGDAHQTMQSIDRAAVSINQAVTQLTSGEGGLSDIKQIIDQTGTAA
                     DKITRSVNSLSQVSLQSSIGLGYHQSGFGEDPQDQGLTAADLRMDLHLNQRDSLVIGV
                     EDIGGSNSSILQWAFTGAGNISRIGLYKNQFGLGIDWLPASNLSLGLNLWDTHSANLG
                     LTSTLKISPYWSVSLGGATNLESRDYSLDLELWRNF"
BASE COUNT          975 a         1053 c         1019 g         1085 t
ORIGIN      
        1 gggtattgct tttttaggaa tttttgttga ttcttcgttg gttattcgtt gagagctgtt
       61 caaagatgca aggatgtgcg gatgttcttt catgattcaa gatgtaagcg cgtgcaattc
      121 ccctgcccct gctgcttatt agcaactcac ccccgcgccc cacggggtgc gctactcacg
      181 cctcatgcct attgccttcc ctgttgcctg taatttcggt tatgctttcg gcacccgttt
      241 cccgccgggt tcatataatg aagcgactga ttgcccggaa aggaggatct ctttatgtat
      301 aagatcttgg cgaaactttt aaacccctat ttaaaaaaaa agggaaagac cgtcatctta
      361 atctggcgcc gtaataagcc ccaagcataa atcgtccgat ccgcctcggc gtggaatgac
      421 taatccggcc tcaaactcac gccgctatgg ggtcactctg ttcttgcccc cggctctccc
      481 ggtccggcgg tatcactatg ccgccgtccg gggccgctcc gcccggccta aacccaataa
      541 ctataataat atgagtgatt ttcccatttt ttattcgacg accgctcggg aatcgatatt
      601 aacattcttg acagattagc cgataccaaa attaaccgaa acttcttcgt tctcctgctt
      661 ttttcccgtc cctttcggac tcggctgtac attccgctat cgcaaaattg aatggacaag
      721 ccctgattga acccattttt gacttcagta aacccttttt tagtgaattt ttaaaatcaa
      781 tatgccgaag atcggtttgg gaacagttca ggatgggctc tggtctcaat atatattaca
      841 ataaaggatt gacaaaaggg gctaacaatg gctgtatcaa aaaagcccac ctggaccggt
      901 tctttaaacc ggctgggacg ggggttattc gtccttctct tcaatatggg tgagatcgcc
      961 cagctttatt tgaaaagttt ccgttcactc ttcagacgga cgctattttg ggagaatacc
     1021 atcactcaga tggagtccat cggagtacga tccctgccct tggtgctaat catctcggca
     1081 tctaccggca tggtcttttc tttgcagatc gccaagatct tcgcaacttt cggcctcacc
     1141 tcccaattgg ggcaaggcct gaccctggcc ttcgcccggg aactcgggcc ggttttaagc
     1201 gccgtaatcg tggctggccg ggttggatcg gcaatagccg ccgaaatcgg ttcgatgaag
     1261 gttacagagc aaattgaggc gctggaaact ctctccaccg atcctatcga ttatctggtg
     1321 gcgccaagaa tcattgccgc aggtttgatg ctcccgattc tggtaatctt cgccgatttc
     1381 atcgggatca tcggcggaat cgcggtggcg gttagttacg ccaatattgc accgacagat
     1441 tttatcgacg ggatattaac ctgggtaact ttctgggatt ttgccggggg gatcattaaa
     1501 gctgtctttt tcggactagt catcgcggga atcggaacct attggggact gagaaccgaa
     1561 aatggcgcgg tcggggtagg aaaagccact accgaatcgg tggtcaccgc ttcgatcatc
     1621 attttcgttt tgaactattt cctctcgatt ctgttgtaca atttataact ttttataagg
     1681 aatatagcct tctccccttt tggcaggaat gtaagtggtc ccttctccgc gaatgtggtg
     1741 gagggcaggt gagggcttac atgcgggtga aaaaatgaac atcatctcct tatataatat
     1801 cgggtatcgg gtcgggggcc agacgatcct ggacggcctg gatcttgagt ttgaaaaggg
     1861 tgaatccttt gtaatcatgg gacgaagcgg ttgcggcaag agcaccctgc tccggttgat
     1921 catcggtttg attcgaccca cctccggttg gatcgaagtg gacggtcgta acacactcaa
     1981 tctaagtccc caggaatggc aggcggtgcg ttatcaaatg gggatggtct ttcaatccgc
     2041 cgctctgttc gattcaatga cgatttatga taatgttgct ttcggtttac gtcgcaaaaa
     2101 tttgcgtgac tccgagatca gggagcgggt ttttgaagct ctgaaaattg tggggctcga
     2161 acctgccact gccctaaaga tgccttccga tctgagcggc ggtatgaaga aacgaaccgc
     2221 aatcgccagg gcgattgctt ttaaaccgcc gatcttactt tacgatgaac ccaccaccgg
     2281 cctggatcca atcatgtcgg atactatcaa tgaattaatc cgggatctcc agcgtaactt
     2341 ggaagtgacc tctatcgtcg ttccgcatga catcatgagt gccttaaagg ttgccgacag
     2401 gattggctta ttaagcgaag gccggctggt ggagctcgcc acccgttccg agttggaccg
     2461 ggaccgccat cctaagcttg ctcaattcat gaagggctat tctttttgaa tcaacagagt
     2521 taaaactcct cggaggtgta gggatgcagt ggaataatcc cgaaacaaaa gtgggtttct
     2581 ttaccctttt agccttgttg gccatgttct ttctctttct ctggctcaac ggttcacagc
     2641 tgttcgagcg tggtcacgag gtggaggcga tctttacccg gattgagggt gtacgtcccg
     2701 gagctccggt taagttctcg ggtgtagata tcggccgggt taccaggatt tattttgagg
     2761 actttaacgt agtggttgtc atgcgaatcc gatccgattt taaagtaccc cgcgctgcca
     2821 aagcggtcat cgccagctcc ggggttgtgg gggacatgtt cattgagatc atgccctacc
     2881 gggtcggaga aaccatcgtt cccatgcgcg gcaaccgctt gaccggacaa aatcccattg
     2941 ccatggatca attctataac accgcctatg aagtgatcgc ctcactgcag caaatcaccg
     3001 attcaattcg gggtcttacc gaaaacccgg aagtaattga atcattacgt caatctttgg
     3061 tccgcctcga cacaattacc aaagatatct cccatatcag cggccaactc gaacagcttg
     3121 actaccccgg ccttttccgg aggatggaaa ataccatgct cattgtggaa cgtctcgccg
     3181 cagccaatga accacggatc aatacattga tcgagaatat cagtctggcc tcggttcaat
     3241 taactcaagc cagtattaca gccaaccgtt tcctcaagga tatagatgcc gatggcgaaa
     3301 ccgccgccga tctccaacag accctggctc atgcggaaca agtggccgcg gatcttgaga
     3361 aactatcgag ttatctcgcc agccataccg gagagattga tcaactgttc ggcgacgccc
     3421 atcaaaccat gcaatcgatc gaccgggccg ctgtgagcat caatcaggca gttacccagt
     3481 taacctccgg tgagggtggc ctttccgata tcaaacagat catcgatcaa accggaactg
     3541 cggccgacaa gatcacccga tcggttaatt ccttgagcca agtctccctc cagagcagca
     3601 tcgggctggg ttaccaccag agcggattcg gtgaagaccc gcaagatcaa gggcttaccg
     3661 cggcagatct ccggatggat cttcatctaa atcaacggga ttctctggtt atcggggttg
     3721 aggatatcgg cggttccaat agttccatcc tgcaatgggc tttcacgggt gcggggaata
     3781 ttagccggat cggtctgtat aaaaaccagt tcggtttagg cattgactgg ttgccggctt
     3841 ccaatctaag cctgggactg aatctctggg atacccactc cgccaatttg ggcctcacct
     3901 caacattgaa aatttctcct tattggtcgg tctctctggg gggcgcaacc aacctcgaat
     3961 cccgcgacta ttcactggat ttggagctgt ggcggaattt ctgacaaaag ccaacgtccg
     4021 gacgacccag tggccgaaga gtgcaaaccc gcaccggaga accaatatta aaaaaccgac
     4081 ccagaagggt cggccttttt tttataaagt ttatttacag agttcgttta ac
//
LOCUS       DASVMB010000453         7236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_646623,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000453 DASVMB010000000
VERSION     DASVMB010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646623"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VF531_12650"
     CDS             complement(<1..904)
                     /locus_tag="VF531_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7714856.1"
                     /translation="MDRIRVGVIGTGLAWERLHYPAYQELSDYYQIAAICDVNQERVE
                     EWGRRLNLNLTRDIFNDCQAMVERDDLQVIDIMVPIAKNHVVAEMAARAGKSVILEKP
                     MGATLEQALGTEELPVRYGVEMMIAENYRYSEEFNILRDLVRQEKVGKPVFFTYHVTN
                     CFPCSMVKDSFAATEWRQHPAYPGGDLLDAAIHPLAGLRHIFGGIKFLQAFGLPQKDD
                     FSPYAAVTVNLSSMSDLIGQFSYYPAGQEPQRPLIGLRIFGTKGMIYLESPECGMINV
                     FYNDGGHEIINYRPMRGYYNELLNF"
     gene            complement(1326..2855)
                     /locus_tag="VF531_12655"
     CDS             complement(1326..2855)
                     /locus_tag="VF531_12655"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7714857.1"
                     /translation="MKQVQIFDTTLRDGEQAAGINLNVQEKLQVARQLARLNVDVIEA
                     GFAIASPGDFAGIQAIARELKGVTVCSLARAVSGDIDRAWEALREAEDPLIHTFIATS
                     EIHMRYKLNKTDDQVLEMAVAAVEHGKKLCSKVEFSAEDATRSDREFLVRIFEAVIRA
                     GATVINIPDTVGYTVPTEFGELLRYLKERVRGIERVRLSVHCHNDLGLAVANSLAAIE
                     NGVDQVECTINGLGERAGNAALEEVVMGLRTRGACFRADTRIRTEQIARTSQLVSTLT
                     GKPVQHNKAIVGANAFAHEAGIHQDGILKNRMTYEIMTPESVGWSKSQLVLGKHSGRH
                     AVKNRLEELGYTLSDPELDRTYQRFIELADKKKEVTDTDLEALIRDENRLMEDHYTLD
                     YLHVSTGNSTIPTATIRLRIKDQLVQEASCGDGPIDAIYKAIDRIAQLPVKLDEYSIK
                     AVTSGQDALGEVMVKVRENGHAYVGRGLSTDIVEASALAYIHALNKIARICNPPSSAT
                     E"
     gene            complement(2978..3103)
                     /locus_tag="VF531_12660"
     CDS             complement(2978..3103)
                     /locus_tag="VF531_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714858.1"
                     /translation="MPSRLGRGTVSRQAAWVLPVTAKTVLLGLRGSWFGYEGRGL"
     gene            complement(3308..4177)
                     /locus_tag="VF531_12665"
     CDS             complement(3308..4177)
                     /locus_tag="VF531_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEX7714859.1"
                     /translation="MYNVVGIRFKQAGKIYYFDPAGFDLKPGMNAIVETARGIEFGQV
                     MIGPKEVPEEKVTLPLKKVLHIATPEDEKQLHENEDKEKRAFGICLQKIEQHQLEMKL
                     VDVEYTFDRSKIIFYFTADGRVDFRELVKDLATVFRTRIELRQIGVRDEAKMLGGLGP
                     CGRPLCCATFLGDFEPVSIKMAKEQNLSLNPVKISGICSRLMCCLKYESSSYRDAKAD
                     LPLVGSKVRVDEREGRIVGNNPLKESLVVEFDDGSRKELLLADVEEIILDNDTGEPKK
                     PKPQVTNDPAADK"
     gene            complement(4297..5241)
                     /gene="holB"
                     /locus_tag="VF531_12670"
     CDS             complement(4297..5241)
                     /gene="holB"
                     /locus_tag="VF531_12670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7714860.1"
                     /translation="MFSDIIGHKDVIQALKGAFREGKVGHAYLFIGPAGVGKKTLARK
                     VAAELLCNGHTARTETNSACECPSCRSFRLASHPDFITVSPAGNSIKIEQLRLLQRNA
                     YLKPLLSNCKVFFFPDAEQLTEAAANSFLKLLEEPPRGVYFLFTAVRGDCILPTIRSR
                     CQVYNLFPVAATEIGPWLEQRGVPQEEAGQRALACHGLPAMALAAEDSSTEATQTKFS
                     AILKKDLLELLSLANELEKEERQSVLERVGEWANQSRLELLRLRSDRAAFSSGELHTR
                     IFVLEKLVKAFEMLEGNANQRLVLEELFLACKIASNRC"
     gene            complement(5318..5767)
                     /locus_tag="VF531_12675"
     CDS             complement(5318..5767)
                     /locus_tag="VF531_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HEX7714861.1"
                     /translation="MEVKPTGPSLDRTQPSETASGRAGGSRQAAFINTLKDSEADQRH
                     QACANLLEQIDAQSSILKKSATPQGIKRYRQLVAAFMKEALGDSYQLNQESHWDRSGN
                     RKTFVTVKRINQDLEELVDSVVNQEKKQIDRVAKLDEIRGLLLDLYL"
     gene            complement(5780..6109)
                     /locus_tag="VF531_12680"
     CDS             complement(5780..6109)
                     /locus_tag="VF531_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX7714862.1"
                     /translation="MRLVIAMIQDQDVNRLLGVLMDHGYSATKLASTGGFLRQGNTTL
                     LIGVADEKVPEVTELIKDTCHSRKQLVTPLASVGRSMNNYMPNPVEVSVGGATIFVVD
                     VLDFAKV"
     gene            complement(6136..6810)
                     /gene="tmk"
                     /locus_tag="VF531_12685"
     CDS             complement(6136..6810)
                     /gene="tmk"
                     /locus_tag="VF531_12685"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7714863.1"
                     /translation="MNDKGFFLTLEGPDGCGKSTQARLLGEYLDEAGCQVLLTREPGG
                     TPLAEEIRRVILTPSRESLDPMTEILLYVAARAQHVKGLILPALASGKVVICERFIDS
                     SLAYQGYGLGWDLELIENLNRMAVGDLSPDLTLLFDQDTEECRKRVQGRAQNKGADRI
                     EARDPAFHQKVREGFLKLAFNNPRFKVISGAGRDIESIQRELRSVLKTRIPKLACLNG
                     TDSNIP"
     gene            complement(6800..>7236)
                     /locus_tag="VF531_12690"
     CDS             complement(6800..>7236)
                     /locus_tag="VF531_12690"
                     /inference="COORDINATES: protein motif:HMM:NF015656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714864.1"
                     /translation="RILERQFRIEPEYSDLNQVTFLVAPWQPKSDWEALYHAVQNLSR
                     HPVNSLQVTMNPPAGVPKLIVKPRLAALSSCMAVPLDEAKGEISAAVVAPYPPGIPLL
                     VPGEVIGPREIDYIHGIRKAGGSVRGLTNNGAILIFRRNNER"
BASE COUNT         1764 a         2048 c         1611 g         1813 t
ORIGIN      
        1 ggaagttcag cagttcatta taatagcccc gcatcggccg gtaattgatg atctcatgcc
       61 ccccgtcatt gtagaaaaca ttaatcatcc cgcactcggg actctccagg tagatcatgc
      121 ctttcgtccc gaagatccgg agcccgatta gaggccgttg cggttcctgt ccggcaggat
      181 aataagaaaa ctgtccgatc aaatcgctca tagaggacaa gttgacggta accgccgcgt
      241 agggactgaa atcatccttt tgcggcaggc cgaatgcctg caaaaacttg attccgccga
      301 aaatatgccg cagtccggcc agaggatgaa tcgccgcatc caacagatcc ccacccgggt
      361 atgccggatg ttgccgccat tcggtggccg cgaaagaatc cttaaccatc gagcagggaa
      421 aacaattggt gacatgatag gtaaagaaga ccggtttccc cactttctcc tggcgcacca
      481 aatcgcgaag aatattaaac tcctcgctgt agcgataatt ctcggcgatc atcatctcca
      541 ccccgtaccg caccggcaat tcttccgttc ccaaagcctg ttccaaggtg gcgcccatcg
      601 gtttttcgag aattacggat tttccggcgc gggccgccat ctccgccacc acatgatttt
      661 tggcaatcgg aaccataata tcgattacct gcagatcatc acgctcaacc atcgcctggc
      721 aatcattgaa tatgtcgcgt gtcaaattca gatttaatct acggccccac tcctcaaccc
      781 gttcctgatt cacatcgcat atggcggcaa tctgatagta atcggaaagc tcctggtacg
      841 caggataatg cagcctttcc caggccaatc ccgttccgat aacgccgaca cgaattcgat
      901 ccattttcta tccccctcac agattaaagc tctaagcttg atgataatct ccccggaccg
      961 gagggggttt atgaatccaa gcgaagctaa aaaatgcaaa agtttatttt caataatggt
     1021 tgccctacgg aatccctttt gcttataata gataaaatac ctcaatggcc gcaattaaag
     1081 ctattcccgt tttcgtgtct ttggtgtgat tcgttgtgaa taaaacaggc ttacgggtag
     1141 aaacttttta gccacgaaaa tcaccaaata cacaaaatgt ctctcttcag acgatatttt
     1201 tagatccgaa aaaatgattc cctgtggccg tattcgatca atcattttca agaacatagc
     1261 gaatgcaaaa aagagcctgc ttggtaaacg caggcctgat atggagaaaa ttccaggtca
     1321 attcatcatt cagttgcaga agatggggga ttgcatatcc gggcgatctt gttgagggcg
     1381 tggatatatg ctaatgcgct ggcctcgacg atatcggtgg ataagccccg tcctacataa
     1441 gcatgaccat tttcacgcac cttgaccatg acctctccca aagcatcctg accggaagtg
     1501 acggccttaa tgctatactc atccagcttc accggaagct gggcgatacg gtctattgcc
     1561 ttatatatgg cgtcaatcgg cccatcaccg caagatgctt cctgaaccaa ctgatctttg
     1621 atccggagtc ggatggtggc agtaggaatg gtggaatttc cggtactcac atgcagatag
     1681 tccagggtgt aatgatcctc catcagacgg ttttcatccc ggatcaatgc ctcgaggtcg
     1741 gtatcggtca cttctttctt tttatccgcc aattcgatga agcgctgata agtacggtcc
     1801 agttccggat cggatagagt ataacccaac tcttccaggc ggttcttcac tgcgtgcctt
     1861 cctgaatgtt tccccaaaac caactggctt ttgctccaac caaccgattc cggggtcata
     1921 atctcataag tcatacggtt ctttaagatt ccgtcttgat gaatcccggc ctcatgagcg
     1981 aatgcattgg ccccgacaat cgccttattg tgctgaaccg gcttaccggt gagggttgat
     2041 accagttggc tggtccgggc gatctgttcg gtccggatac gagtatccgc tcggaaacaa
     2101 gcgccgcgcg ttcttagacc catgaccacc tcttctagag ccgcatttcc ggcccgttct
     2161 cccaaaccgt tgatggtaca ctcaacctga tccactccgt tttcgatcgc tgccagcgag
     2221 ttggctacag ccagacccag atcattgtgg cagtggaccg aaagccgtac tctttcaatc
     2281 ccgcggacac gctctttcag ataacggagt aactccccga actcggtggg gaccgtgtaa
     2341 cccaccgtat cgggtatatt gatcactgtt gcgccggccc ggattaccgc ttcaaagatc
     2401 cggaccagaa actcacgatc actgcgggtg gcgtcctcgg ccgaaaattc cacctttgag
     2461 catagttttt ttccgtgctc gacagcggct acggccatct ccagcacctg atcatctgtt
     2521 ttgttcaatt tatagcgcat atgaatctcg gaagttgcga tgaaggtatg gatcaacgga
     2581 tcttcggcct cgcgtaaagc ctcccaggcc cgatcgatat ccccggaaac agcccgtgcc
     2641 aacgaacaaa cggtaacccc cttgagctct cgggcaatcg cctgaatccc ggcaaaatca
     2701 ccgggtgagg cgatggcaaa ccctgcctcg ataacatcca cattcaacct ggccagctgc
     2761 cttgcaacct gtagcttctc ctggacattc agattgatac ccgccgcttg ctctccatcc
     2821 cggagcgtag tgtcgaagat ttgaacctgt ttcacgaagt tacctcctct ttcttattca
     2881 tttgcgccca tgccgggcgc acataaaaaa ccccgtcccg aaatatcggg acgaggttta
     2941 agttagactc gtgttaccac ccatttttac cggagcttta caacccgcgg ccttcataac
     3001 cgaaccagga gccacgcagg cccaagagaa cggttttcgc tgtaacgggc aggacccaag
     3061 cggcttgacg gcttacggtc ccccggccca acctactcgg catcccgaaa gatgccttta
     3121 ggtcggcggc tcggagggga gtttcgaaag ttcgggcacc ggctcacacc tttgcacgct
     3181 cccgtgctcc accggctctc ttcagccctg aaattctact agcctcttca tcgtcgttca
     3241 agatatattt atccattatt atatggaaga atcttcatca tgtcaagtca tttctcactc
     3301 cgcacactta tttatcggct gcaggatcat ttgtaacttg gggtttgggt tttttcggtt
     3361 caccggtgtc attatccaag attatctcct ctacatcggc caagagaagt tcctttctgg
     3421 aaccgtcgtc aaactcaact accagagact ccttgagcgg gttatttcct acgatccttc
     3481 cctctcgctc atctacccga actttactcc ccaccagcgg tagatcggcc ttggcatcgc
     3541 gataactgga cgattcatac tttaggcagc acatcaacct ggaacagatc ccggagattt
     3601 taaccggatt gagtgaaagg ttctgttctt tagccatttt aatcgaaacc ggttcaaaat
     3661 ctcccaagaa agtggcgcaa cacagcggcc ggccacacgg accgagtcct ccaagcatct
     3721 tggcttcatc ccggactccg atctgacgga gttcgatgcg ggtccggaat accgtcgcca
     3781 agtccttgac caattcccga aagtccaccc ggccgtcggc ggtgaagtag aaaatgatct
     3841 tactccgatc gaaggtatac tccacatcca ccagtttcat ctccagctgg tgctgttcga
     3901 tcttctgcag acatataccg aaggcgcgct tctctttatc ttcattttcg tgtaattgct
     3961 tttcatcctc aggagtggcg atatgcaaca ccttttttag gggtaaggtg actttctcct
     4021 ccggaacttc tttgggaccg atcatcacct gaccgaactc aatcccccgg gcggtttcca
     4081 cgatagcgtt cattcccggt ttcaaatcaa aaccggcggg atcaaaataa tagatcttac
     4141 ccgcctgctt aaaccggatg cctacaacat tgtacataat aactccaaat cctttcatcg
     4201 gccgcagggt ctcttgcatt catcgctccg agaccctaac ggcacgtcat aatcactttt
     4261 atcggtaaag gctatcctca gtaaaaatcg gttaacttag caacggtttg acgcaatctt
     4321 acaggccaaa aacagttcct cgagtaccaa tcgctgattg gcgtttcctt caagcatctc
     4381 aaaagccttg accaactttt ccagaacaaa aattcgggta tggagctctc cggaactaaa
     4441 tgccgcccga tcgctcctta accggagtaa ttcgagacgt gattgattgg cccactctcc
     4501 gacccgctcc aaaaccgatt gacgttcttc tttctccagc tcattggcga gacttagcag
     4561 ttccagcaga tcctttttta aaatcgcgct aaacttagtc tgtgtcgctt cggtggaact
     4621 atcctccgct gccaatgcca tagccggaag tccatgacat gccagcgccc gttgcccggc
     4681 ttcctcttgg ggaacgccgc gttgttccaa ccaagggcct atctccgtgg ctgccaccgg
     4741 gaagaggtta taaacctggc aacgtgaacg gatcgtaggt aaaatgcaat ccccgcggac
     4801 tgcagtaaag agaaaataaa cccctcgcgg cggttcctcc agtaatttca agaaactgtt
     4861 ggctgcagcc tcggttagtt gttccgcatc gggaaagaag aatactttac agttgctaag
     4921 gagcggctta agataggcgt tacgctgcaa aagccggagt tgctcgatct tgatcgaatt
     4981 gcccgccgga ctcaccgtaa taaaatcagg atgtgacgcc aaccgaaaac tacggcatga
     5041 aggacattca cacgcgctat tggtctctgt ccgggcggta tggccgttac agagcagttc
     5101 tgcagcaacc ttcctggcta atgtcttctt gcctacaccc gcagggccga taaacagata
     5161 ggcatgtcct accttcccct cacgaaacgc cccttttaac gcttggatta catctttatg
     5221 acctattata tcagaaaaca accttgcccg cctcgattgt taaaaattgt tcgccgtgaa
     5281 acaatttggc ctgaaacatt tggaatgccg gaaagcctca taaatacaaa tctaacagca
     5341 acccgcggat ctcatcgagc ttcgctaccc gatcaatctg tttcttctcc tgattgacaa
     5401 cagagtccac caattcctcc aggtcctggt tgatccgttt cacagtaaca aatgtcttcc
     5461 ggttgccact tcgatcccaa tgggactcct gattgagctg ataggaatct ccaagggcct
     5521 ctttcatgaa agccgccact aattgacggt agcgcttaat tccctggggt gtggcggatt
     5581 tcttcaatat cgagctctgg gcatcaatct gctctaaaag attggcgcac gcctggtgcc
     5641 tctgatcagc ctcggaatcc ttcagtgtat tgatgaaagc agcctgacgg gatcctccag
     5701 ccctgccgga tgcggtttcg ctcggttggg ttcgatcaag actaggcccg gtgggtttaa
     5761 cttccattgg cattattttt cacactttcg cgaaatcaag cacatcaacc acgaaaatcg
     5821 tggccccgcc aaccgaaacc tccactggat tgggcatata attattcatt gaccggccaa
     5881 ccgaagcaag cggcgtaacc agctgtttcc gggaatgaca ggtatccttg attaattccg
     5941 taacttcagg aaccttctca tcggcgacac caattagaag tgttgtgttg ccctggcgta
     6001 aaaatccacc ggtacttgcg agtttagtgg cggagtaacc gtgatccatc aatactccca
     6061 aaagccgatt aacatcttga tcctggatca tcgcaatgac taaacgcatc cccgcatcct
     6121 ccccttttcg gatttttatg gaatattcga atcggtccca tttaagcatg ccagcttcgg
     6181 tatccgggtt tttaggaccg agcgcaactc acgctggatg gactcaatat ctcttccggc
     6241 cccggatatt accttaaacc ttggattatt aaacgccagc ttaagaaatc cctcccggac
     6301 cttctgatga aatgccgggt cccgtgcctc gatccggtct gcccctttgt tctgggcacg
     6361 accttgtacc cttttgcggc attcttcggt gtcttgatca aacaaaagcg taagatccgg
     6421 agacagatct ccaacggcca tccggttaag attttcaatc agctccaaat cccaacccaa
     6481 tccataaccc tggtatgcca agctggaatc gataaagcgt tcacagatga ctactttacc
     6541 ggaggctaac gccggaagga tcaacccttt gacatgctgg gcccgggctg caacatatag
     6601 caaaatctcg gtcatcggat caaggctttc gcggctcggc gtcaaaatta ctcgccgtat
     6661 ctcttcagca agcggagttc cccccggttc ccttgtcaag aggacctgac agcccgcctc
     6721 gtccagatac tccccaagca accgggcttg agtcgactta ccgcatccgt ccggtccctc
     6781 cagggttaga aaaaaacctt tatcgttcat tgtttctcct aaaaatcagg atcgcgccgt
     6841 tatttgtcag cccgcgaacg ctacctccgg ccttacggat cccatggata taatcgattt
     6901 ccctagggcc gataacttca cccggaacca gtaacggaat tcccggcgga tatggtgcta
     6961 cgactgcggc cgagatttca cctttcgcct catcgagcgg tacggccata caggaagaaa
     7021 gcgcggcaag ccgtggcttt acgattaact tgggaacacc tgccggggga ttcatcgtga
     7081 cctgtaaact gtttacagga tgacggctga ggttctgaac cgcatggtac agagcctccc
     7141 aatccgattt tggctgccaa ggcgcaacca aaaaagtaac ctgattcaga tcggaatatt
     7201 ccggctcgat ccgaaattga cgttccaaaa ttctcg
//
LOCUS       DASVMB010000454         8789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_646796,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000454 DASVMB010000000
VERSION     DASVMB010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646796"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VF531_12695"
     CDS             <1..1107
                     /locus_tag="VF531_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7714865.1"
                     /translation="PKEEWPGIIRRELTQAVLKLFAMVIGCGLVGAALMLLAFHIRPV
                     SLRFWQGILVSFGCILLLIGTTVWTYDPHAIEHPQYHGVLASAPWMMNLVSMSIDHIE
                     VIGSNLRKISRDLPLLYKQAGEIQNLGQFDTDLAVLHVSDIHNNPAAFEFLSQLVNDF
                     KIRFIVDTGDLTDYGTPLEAKILDQFRAIKVPYVFIPGNHDSPLIIEKLRRLRRVRVL
                     QSGTLKLEGLTIAAMADPASGSYSSDVSSPEKLAKSGQQLAEQVTALKQSPDIVAVHN
                     RLLATGLIGKVPLVIYGHDHRYNLATESGTVTDDAGTTGAAGIRGLEEKNIPYSATIL
                     YWEKDSGGSLRLRAADSIRINGSEGFLTINRQTF"
     gene            1409..3361
                     /locus_tag="VF531_12700"
     CDS             1409..3361
                     /locus_tag="VF531_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7714866.1"
                     /translation="MISLAYGADFNSELILAAARGQVGTVKTLISKGANVNARDYNNW
                     TALMHASAKGQTKVVEALIQNGANVNLKDNDDWNALMYGVDNNHLPVVELLVDNGADI
                     NPRNKDGYTPLMLAVKNNTSKIAKYLIDNGGNYTLSLLYASRKGDTSTVTTLLKLYPA
                     GLNELDDNGWNALLWTAWNGFTDTLQVLLAQGADVNFQSKDGYTPLLAAASQGNMDIV
                     QLLLPRASTLQVVDPNGRSILFWEASRGHETIVKALLAKGVPVNQKDHSGSTPLIAAA
                     KGDYAPTVEALLAQGAAVGATDSDGWSALHWAAKQGNAETAQALIRRKADINLKNKSG
                     QTPLIIAALAGNNDMVRLLLIRGAAVNLTDSQGRTALTCALNNGHPETAKILADQGAA
                     VKEPDPTPSAKPAETPKPTETPVPTVTSTPTNSLSQSLLDAVQHDDPAALEQALRAGA
                     DVNARDAQGLPMIFSAVKNAAPATVKILIDGGAAVNVADKYGWSPLMWAAARGDLAIV
                     KMLLDKGADIHQKTTKYGWTALTIAAQNGRSAVASTLIIAGADVNQRDKEDDTVLILT
                     VMEEHLDTVELLLANGAKVNLKGKDGYTALMMAAYKGNTDLVKLLLKHGADFKLKNKY
                     GYTALAIALAKGHSKAAQVLRSAGAK"
     gene            complement(3490..5331)
                     /gene="typA"
                     /locus_tag="VF531_12705"
     CDS             complement(3490..5331)
                     /gene="typA"
                     /locus_tag="VF531_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7714867.1"
                     /translation="MIKKKENIRNVAIIAHVDHGKTTLVDCLLRQSGIFRDNEKVVER
                     VMDSNELERERGITILSKNTSVNYGATKINIVDTPGHADFGGEVERVLRMVDGALLLV
                     DAFEGPMAQTKFVLRKALEIGLRIIVVINKIDRPEARPNEVLDEVFDLFVELEASDAQ
                     LDFPVIYTSARLGIAVLDPGETKGDMRSIFEMIQKEIPEPSGDLEHPLQLQITTLDYD
                     DYVGRIGIGRINNGVIHASQQVSICKQDGGVEMGKIGKLWTFEGLKRAEVEEAAVGDI
                     VAICGLEKVNIGETICEADKPMALPLLTIDEPTLTMTFMVNDSPFAGREGKYLTSRHL
                     RDRLWKEAQTNVSLKVTETDSPDAFQVSGRGELHLAILIETMRREGYELQVSKPEPIL
                     KQINGQLCEPYERLFINVPDAFSGTTIENLGRRRAELLNMQPIGQSHVKLEFLIPSRG
                     LIGFRSEFLTETKGEGIMNHLFDHYGPWKGDIPGRLRGSLVAFETGDACTYGIHNVED
                     RGVLFVSPTDKVYAGMIVGENARENDLDVNVCKKKHLTNMRSSTSDETIRLTPPRVFS
                     LEQAMEFIADDELVEITPNSIRMRKKVLDRADRGRLAKRKVAEPDAG"
     gene            5678..6772
                     /gene="ychF"
                     /locus_tag="VF531_12710"
     CDS             5678..6772
                     /gene="ychF"
                     /locus_tag="VF531_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7714868.1"
                     /translation="MQLGIVGLPNVGKSTLFNAITKAGAECANYPFCTIEPNVGVVPV
                     PDLRLTRLAAMYDPKKITPATVEFVDIAGLVRGASKGEGLGNKFLAHIREVDAIVHLV
                     RCFEDPNIVHVDGSVAPLRDVETINLELIFADLETLEKRMDRTGKMLKTGDKKYQTEM
                     AVLEALKNHLESGQPARTFQLTEEQREMLADLFLLTSKPVLYVANIAEPDLQPGSANP
                     YLEALEQSVAKENSEVIPICARIEEEIAQLPESERQEYLTALGLSESGLNRLIKASYK
                     LLGLISFLTAGPQEVRAWTIVRGSKAPQAAGKIHSDFERGFIRAEIVAYDDLMACGSY
                     NAAKEKGLVRSEGKDYVMQDGDVTLFRFNV"
     gene            7120..7485
                     /locus_tag="VF531_12715"
     CDS             7120..7485
                     /locus_tag="VF531_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714869.1"
                     /translation="MVAEKKFKLEVDNLRNYFIIKVWGFFTADDANDFIQSYQEAIKT
                     FNPNDYTLVVDGRDLKTSSPDVAAMLQKVMQMYIQTPFKKRYSIELNSLLTKMQLQRL
                     GQDVIFQYFTFVNSESEIQ"
     gene            7741..8745
                     /locus_tag="VF531_12720"
     CDS             7741..8745
                     /locus_tag="VF531_12720"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX7714870.1"
                     /translation="MVQNVIIKGVGIFHPTRKVENEFYIEHFKKQGNDITHLLNHLGR
                     KTRFLVDDENETAITLAVKAAKDALSNAHIKAEDLDMIVFVSDNPHYLSPTNALMINH
                     ELGAKNAHIVYDMNSNCTGAVTAIDQVSRYLKTNKRIRYALIAGSMFITPFARESDPI
                     VYAGAGDAGGCVILENVSGQKRVGFIDSNYFTDSNYNYTIVYPQCGLPNVVKNSVDED
                     KKKLNWTSFDFDFLCDKWAGLITEMAKRNHVTIDQIGHYIFSQFSRADIIKTIDKLQV
                     SHDKYTFIADKYGYTGVTSPIFTFYNALKNGAIKNNSYVVFCSVGAGYTMSSILYLQP
                     "
BASE COUNT         2280 a         2276 c         2083 g         2150 t
ORIGIN      
        1 cccaaggagg aatggcccgg aatcatccgg cgcgaattga cccaagcagt attgaaactc
       61 ttcgcaatgg tcatcgggtg cgggctcgtc ggcgccgccc ttatgttgct ggcatttcat
      121 attcgtcccg tttcacttcg gttctggcag gggatactgg tttcattcgg ctgtatcctg
      181 ctattaatcg ggactacagt ctggacctac gaccctcatg ccatcgaaca cccccaatat
      241 cacggggtgc tggcctcggc cccctggatg atgaatctgg tctcgatgag tatcgatcat
      301 attgaagtaa ttgggagcaa tctccgcaag atctcccggg acctgccgct cctttacaaa
      361 caggccggcg agattcaaaa cctgggtcaa ttcgataccg atcttgcggt actccatgtc
      421 tcggatatcc ataacaatcc ggcggctttt gagttcctct cccaactggt gaacgatttt
      481 aagatccgct ttatagtgga tacgggcgat ctaaccgatt acggcacccc gcttgaggcc
      541 aaaattcttg accaattccg cgccatcaag gtgccctatg tgttcatccc cggcaatcac
      601 gattcgccgc tgatcatcga gaagttgcgg cggttacgcc gggtaagggt tctgcaatcc
      661 ggcaccttga aactcgaggg acttaccatt gcggcgatgg ccgatccggc ctccgggagt
      721 tacagctccg atgttagttc cccggagaag ctggccaagt ccggtcaaca actggctgaa
      781 caagtgaccg ccctgaaaca atcaccggat atcgtggcgg tccataaccg gttgctggca
      841 accggactca tcggtaaggt tccgttggtg atatacggtc atgatcaccg gtacaacctg
      901 gcgacggaat cgggcacagt gaccgatgac gccggaacta ccggcgcggc agggatccgg
      961 ggccttgagg agaaaaatat tccttacagc gccacgattt tatactggga aaaagattcc
     1021 ggagggtctt tacggctccg ggccgcggac tcaatccgga ttaacggcag cgaaggcttt
     1081 ttgaccatca atcgccagac cttttagccc gggtcttcgc ggcggattaa tcacccgcca
     1141 aacggcaggg ttttccacct atcatgtgga atattgccga agatgcaatt tgcttataaa
     1201 tatttagcca tccggggccg agaatgatca tgacaatgga taaacaacgg ggtcaccgac
     1261 ccctttgatg cggaatctaa cctctgttct caaagaaggt gtagctatga atacccagaa
     1321 ccaccggctc cggcgaggcg ctcttcacgg atgtcgtcgc gcgcgtgtgc gcgatttttt
     1381 tctccttgcg gcgttgggag tatcaatcat gatttcactg gcctacggcg ccgatttcaa
     1441 ttcggagtta atccttgccg ctgcccgcgg tcaagttggc accgtaaaaa cattgatttc
     1501 caagggtgct aatgtcaacg cccgtgacta caacaactgg accgccctga tgcacgcctc
     1561 ggccaaaggc cagaccaaag tggttgaggc cttaattcaa aacggggcca atgtcaatct
     1621 caaagacaat gacgactgga atgctttgat gtatggtgtc gataacaatc atcttccggt
     1681 agtggaatta ctggtggata acggtgccga tatcaacccg cggaataagg acggctacac
     1741 cccgttaatg ctggccgtta agaacaacac ctccaaaata gccaaatacc tgatagataa
     1801 cggcggcaat tacaccttaa gcctgctcta tgcttcacga aaaggtgata cttccacggt
     1861 tacaaccttg ttgaaacttt accccgccgg cctgaatgaa ttggacgaca acggatggaa
     1921 tgcattgctc tggacggcct ggaacggttt caccgataca cttcaagtat tgctcgccca
     1981 aggagcggat gttaactttc aaagcaaaga cggttacacc cccctactgg cagcggcgag
     2041 tcaaggcaat atggatattg tccaactact cctgcccaga gcctccaccc ttcaagtcgt
     2101 agatcccaac ggtcgatcga ttcttttctg ggaggcttcc cggggtcatg agacgatagt
     2161 caaagcctta ctggccaaag gtgttccggt gaatcagaaa gatcattccg gttccacacc
     2221 cctgatagcc gcggccaagg gtgattatgc ccctacagtc gaggcgttat tggcccaggg
     2281 agccgcggtc ggcgccaccg attccgacgg ttggtcggca ttacattggg cggccaaaca
     2341 gggcaatgcc gaaacagcgc aagccctcat ccgccgtaaa gccgatatca atctcaagaa
     2401 taaatccggc caaactccgt taatcatcgc ggcacttgcc ggaaataacg acatggtaag
     2461 gctgctgctc atccgtgggg cagcggtcaa cctcaccgat tcgcaaggcc ggaccgccct
     2521 aacctgcgca ctcaataatg gtcatcccga gaccgccaaa atattggcgg atcaaggcgc
     2581 tgccgtgaaa gagcccgacc cgacaccatc agcgaaaccg gcggaaactc cgaagccaac
     2641 tgagactcca gtgccaacag taacttcaac tcctacaaac tcgctaagcc agtcgttact
     2701 cgatgcggtg cagcacgacg acccggccgc tttggaacag gcgctccgtg ccggcgcaga
     2761 tgtcaacgcc agggacgctc agggattacc catgatcttc agcgcagtaa agaacgctgc
     2821 tccggcaacc gttaaaatcc tgattgatgg tggcgcggcc gtaaacgtcg ccgacaaata
     2881 cggctggtcg cccctgatgt gggctgcagc caggggtgat ctggcaatcg tcaaaatgtt
     2941 gctggacaaa ggggccgaca tccaccaaaa gaccaccaaa tacggctgga ccgccctgac
     3001 tatcgccgcc cagaatggcc ggtccgccgt ggcttccacc ttgattatcg ccggcgccga
     3061 tgtgaatcaa cgcgataaag aagacgatac cgtcctgatc ttgaccgtca tggaagaaca
     3121 tctggatacg gtcgagttgt tgttggcgaa cggcgccaaa gtcaacctca aggggaagga
     3181 tggatatacc gccttgatga tggcggctta taagggcaac accgacctgg tcaaactgtt
     3241 gttgaaacac ggcgccgact tcaagctgaa aaacaagtac ggatataccg ccttagccat
     3301 cgccctcgcc aagggtcatt ccaaggccgc tcaagttttg cgtagtgcag gagcgaagta
     3361 agaaatgtga agagtgaaaa acaaaacagt cattccggaa ctttatttca cccatcaaaa
     3421 aagacggacc caaataactc aggtccgtct ttttattact ttggttaatc tttactttgt
     3481 gcagctctat caacccgcat ccggttcggc aaccttccgc ttggccaggc gcccgcgatc
     3541 cgcccggtcc aaaaccttct tacgcatccg gatggaattg ggagtgatct ccaccaattc
     3601 atcatcggca ataaactcca tcgcctgctc cagactgaag actcggggcg gagtcagccg
     3661 gatcgtctca tcggaggtac tggaccgcat attggtaaga tgcttcttct tgcagacatt
     3721 gacgtccaga tcgttttccc gcgcgttctc gccgacgatc atcccggcgt ataccttgtc
     3781 cgtgggactc acgaaaagca ctccccggtc ctccacgtta tgaatcccgt aagtgcacgc
     3841 gtcaccggtc tcaaaagcca ccagcgatcc gcgaagtcgt ccgggaatat cccccttcca
     3901 ggggccgtag tgatcgaaga gatgattcat aatcccttcg cccttggttt ccgtgagaaa
     3961 ctcggagcgg aaaccgatca ggccgcggga gggaatcaaa aactccagtt tcacgtggct
     4021 ctggcctatc ggttgcatat tcaggagttc ggcgcggcgc cgcccaagat tttcaatagt
     4081 ggttccggaa aacgcatccg gcacgttgat gaagagacgc tcgtacggtt cacacagctg
     4141 gccgttgatc tgctttaaaa ttggctccgg tttggaaacc tgcagttcat acccttcacg
     4201 gcgcattgtt tcaatcaaaa tcgccaagtg cagttcgcca cggcccgaga cctggaaagc
     4261 atcgggcgaa tcggtctccg tcaccttcaa actcacgttg gtttgggctt ccttccaaag
     4321 ccggtcacga agatggcgcg aagttaggta tttaccctct ctgcccgcga agggactgtc
     4381 attgaccatg aatgtcattg tcagtgtagg ttcgtcgata gtcaacaacg gtaaggccat
     4441 aggtttatcc gcttcacaga tggtctcgcc gatatttacc ttctcaaggc cgcaaatagc
     4501 cacaatatca ccgaccgcag cctcttcgac ctccgctcgt ttcaaacctt caaaggtcca
     4561 gagcttgccg atcttcccca tctccacgcc gccgtcctgt ttacagatac tcacctgttg
     4621 gctggcatgg atgaccccgt tattgatccg gccgatcccg atcctgccca cgtaatcatc
     4681 ataatccaaa gtagtgatct gcagctgcag cggatgttcc agatccccgc taggttccgg
     4741 aatctctttt tgaatcattt caaaaatcga ccgcatgtcg ccttttgtct caccgggatc
     4801 aagtacggca attcccaaac gggccgaggt gtaaatcacc ggaaagtcga gttgggcatc
     4861 ggaagcctca agttccacga aaaggtcgaa gacctcatcc aaaacctcat tgggacgggc
     4921 ttccggccgg tcgatcttat taattactac aataatccgg aggccgatct ctaaggcctt
     4981 gcggagcacg aacttggttt gggccatcgg gccttcaaag gcgtccacta ataataacgc
     5041 cccatcaacc atccgcagta cccgttccac ctctccaccg aaatcggcat gtccgggcgt
     5101 atcgacaata ttgatcttgg tagcgccgta attcaccgag gtgtttttgg aaagaatggt
     5161 gataccgcgc tcccgttcca gctcgttgct gtccatcacc cgttcgacca ctttttcatt
     5221 atcgcgaaag atacccgatt gccgtaataa acaatcgacc agcgtggttt tgccgtggtc
     5281 aacgtgagcg atgatggcaa catttcggat gttttctttt ttcttaatca ttacgtcatt
     5341 tcacccaatc ttatcaatct tcacatcccc ttattaaagc acgaattcgt tcaaattaca
     5401 ataaaaaaat ctttaatttt taggcctgaa ctggctgatt atctttgttc aggcagtttt
     5461 ttcggtttga ctcataaaat taccaaccaa gctcggttaa tctcccatac ccttgcatgc
     5521 ccgctccacc caaagattcc ttacgctccc actatgcgat tgaatttttt gagtggatca
     5581 catataatga aggttattga aaatctttat cgcgctgttt attttgttca gccccgggaa
     5641 gaacgacggc tgaatctaga gaaaggattt ggaaagtatg caactgggaa ttgtgggact
     5701 gccgaatgtc ggcaaaagta ccctttttaa cgcgattacc aaagcaggcg ccgaatgtgc
     5761 caattatccg ttttgtacca tcgagccgaa cgtcggggtg gtgccggttc cggatctccg
     5821 cctgacccgc ctggccgcca tgtatgaccc gaaaaaaatc actccggcga ctgtggaatt
     5881 tgtggatatc gccggtttag tccggggcgc cagcaaaggg gaaggccttg gcaataaatt
     5941 cctggcccat atccgcgagg tggatgccat cgtccatctg gtccgctgtt ttgaggatcc
     6001 caatatcgtc catgtggacg gttcggtcgc accactccgg gatgtcgaaa ccattaacct
     6061 ggaattgatc tttgccgatc tggaaactct ggagaagcgg atggatcgca ccggtaagat
     6121 gctgaagacc ggagacaaaa agtaccagac ggagatggcg gttctagagg ccttaaagaa
     6181 ccatctcgaa tcgggccaac cggcccggac ctttcaactc accgaagagc aacgtgaaat
     6241 gctggccgat ctctttttac tcacctcaaa accagtactc tatgtggcta acatcgccga
     6301 gccggacctg caacccgggt ctgccaatcc ctacctggag gctttggaac aatccgttgc
     6361 caaggagaac tcggaagtta ttcccatctg cgcccggatt gaggaagaaa tcgctcaact
     6421 tcccgagtct gaaaggcaag agtaccttac tgcactgggg ctttccgaat ccggtttgaa
     6481 ccggctgatt aaggccagtt ataaactgtt gggcctaatc agtttcctta ccgccggtcc
     6541 ccaagaggta cgggcttgga cgattgtcag gggctcaaaa gccccgcagg ctgcgggcaa
     6601 gattcacagc gacttcgaac ggggcttcat ccgggccgag attgtggctt acgacgacct
     6661 gatggcctgc ggctcctata atgcggccaa ggaaaaggga ttggtccgct ccgagggtaa
     6721 agactatgtg atgcaggatg gggacgtgac cctgttccgc tttaatgttt aggattccaa
     6781 caaaccttcc ccaagagagc gaaagggcgc gaagatcgcg cccttttttt tacctatttc
     6841 cgatgtaagt acggctattc cagattagct cataacattg cattagtccc ctaagtttaa
     6901 atttactttt ttgaaaaaat actctcccaa gttattgaaa gcaagtattt attgtaaaaa
     6961 atttcctatc aaattgcata atatactatt tacacagatt ggtcaattct tacttacaag
     7021 ttgacaataa aatctttaca ttaatatccc aaaaagaagg attttttcct gattttcacg
     7081 aattgttctc acactaatct attttaagga gttgaaaaga tggttgccga gaaaaaattc
     7141 aaattagagg ttgacaattt aagaaattac tttattatta aggtttgggg attttttaca
     7201 gcggatgatg ccaacgactt tatccaatcc taccaagagg caattaagac ctttaatccg
     7261 aatgattaca cactggtggt tgacggaagg gatctcaaga cttccagccc ggatgtagcg
     7321 gcaatgttgc aaaaagtaat gcagatgtat atccagactc ctttcaaaaa aaggtattcc
     7381 atcgaattaa actctctatt aaccaaaatg cagttacagc gtttaggaca ggatgtcatt
     7441 tttcagtatt ttacatttgt gaattctgaa tcggaaatcc aataaataaa ataattagca
     7501 ttgtttaaag aaacggttac tcccaaatat aatagttggg tgttgagagc agagcatcat
     7561 gttctaatgg gcgcggtgct ctggtttaat gtctatagga cattgtaggg tcattacccc
     7621 ctataaatag caaaatgaga ctatcggatt aaccgttact gcgaacattg ctaaatgaat
     7681 aacgcatcac gaagtgtctt caaaagcaag acactaattt tttttgaaag gggatcgtta
     7741 atggttcaga atgttatcat taagggtgtt ggaatctttc atcccacccg gaaggtagaa
     7801 aacgagttct atattgaaca ttttaaaaag caaggcaatg atataaccca tttactgaac
     7861 catctagggc gaaaaacccg gtttttagtt gatgatgaaa atgaaaccgc cataacactt
     7921 gcagtaaaag cggccaaaga tgcgttatcg aatgctcata ttaaagccga agatttagat
     7981 atgattgtat ttgtatcgga taaccctcat tacttatccc ccaccaatgc cttaatgatt
     8041 aaccacgaat taggtgcgaa aaacgcgcat atcgtatatg atatgaattc aaactgcacc
     8101 ggcgcagtta ccgccatcga tcaagtcagc cgctatttga aaaccaataa gcggatcaga
     8161 tatgccttaa ttgcgggcag tatgttcatc acgccctttg cgagagagtc ggatcccatt
     8221 gtctatgccg gtgcgggaga cgcgggcggt tgcgtcattt tagaaaacgt ttccgggcaa
     8281 aaacgggtgg gatttatcga ttccaactat tttacagatt cgaattacaa ttacaccatt
     8341 gtttacccgc aatgcggctt accgaatgta gtaaaaaaca gtgttgacga agacaaaaag
     8401 aagctgaact ggacctcgtt tgattttgat ttcttatgcg acaaatgggc cggtttgatt
     8461 accgaaatgg ccaaacgcaa ccatgttacc attgatcaaa tcgggcatta tatcttctcc
     8521 cagttttcca gggcggacat catcaagacc atcgataaat tgcaagtctc ccacgacaaa
     8581 tatactttta ttgcggacaa gtatggctat accggggtta ccagcccgat attcactttc
     8641 tataatgcac tgaaaaacgg ggctatcaaa aataattcct atgttgtttt ctgttcggtg
     8701 ggagccggtt ataccatgtc gtccatcctc tatttacagc cgtaagttta cccatcaaaa
     8761 taaatccggg acgttttgtc ccggattta
//
LOCUS       DASVMB010000455         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_279106,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000455 DASVMB010000000
VERSION     DASVMB010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279106"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..578)
                     /locus_tag="VF531_12725"
     CDS             complement(276..578)
                     /locus_tag="VF531_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX7714871.1"
                     /translation="MATQYNDDRVSIEPAPVQSGDTVHINYQGILKNSGADQVFLHYG
                     VDGWRNINTVPMQQQAGGVFGAEVRATGSQELNFCFKDSADHWDSNSGWNWKVEVR"
     gene            776..1465
                     /locus_tag="VF531_12730"
     CDS             776..1465
                     /locus_tag="VF531_12730"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7714872.1"
                     /translation="MDERTGKKAFQEVVARLEALIKTGQVGFGEYLPPERELAGRFGV
                     GRPAVREALKALEILGMVVIEHGKGARVTTPSLESWFQAIGTSLVLSPADFLHLMEAR
                     ETLEPQLAFIAASRATAEQLQELGSSLTVMNRALEKVTEFAAADYRFHGLIAQATNNP
                     VLIKIYEAIAGLLWLLQQETAVVPDRQKSYTYHEAIYRAVFERNPMVAREEMELHLKD
                     TRERFLDFRPE"
     gene            1543..2691
                     /gene="dgoD"
                     /locus_tag="VF531_12735"
     CDS             1543..2691
                     /gene="dgoD"
                     /locus_tag="VF531_12735"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX7714873.1"
                     /translation="MKVAAYELFMVKPRWLFLRLETDTGLVGWGEPVVEGRAETVKAA
                     VQELMDYVMGQDPSRIEDLWQVMYRGGFYRGGPILTSAISGIDQALWDIKGKYYNAPI
                     YELLGGAVRERVRVYTWIGGDRPAEVKQEALRKLQAGFTAVKMNATEEMQRIDSPAAV
                     DEVLARLAAVREAVGPKFDIGLDFHGRVSKTMAKILAREMEPYHPFFIEEPVLAENNE
                     ALREIASATTIPIATGERQFTRWGFKQVLIDGHVDLIQPDISHCGGISELKKIAAMAE
                     TFDVAVAPHCPLGPIALAASLQLDFCTPNVLIQEQSLGIHYNQGSDLLDYLEDRTVFD
                     YRDGYVSRMTGPGLGIRIDEAKVREAAREGHRWRNPVWRHADGTVAEW"
BASE COUNT          702 a          680 c          824 g          729 t
ORIGIN      
        1 gactccattt tggcgcttcc tgctaatttt gaaacttacc atattttgga ccggagggaa
       61 cgggccagtg aaagatgcaa ttcaccatga ccatttcttt atattatata atatcggata
      121 gttattttta tgcctgatta tcgccgtttt gtttcattcc tcgcagcaat cccgcgatat
      181 aacgcaatga atccccgggg caggggtgaa aaggggggaa tattgcctgt agatttgaaa
      241 ttaaaaacga aacgccggtc ttcccggcgc ttcgtttagc ggacttccac cttccagttc
      301 cagccactgt tggaatccca atgatcggcg ctgtccttaa agcagaaatt caattcttga
      361 ctgcctgtgg cacggacctc ggcaccgaaa actcctcccg cttgttgctg catcggtacg
      421 gtgttgatgt ttctccagcc atcgactccg taatggagaa aaacctggtc ggctccggaa
      481 tttttcaaga ttccctggta attgatatgc accgtgtcac cggattggac cggggccggc
      541 tcaatcgata cccgatcgtc attatactgg gtcgccattc tgtatcgcct ccttcattca
      601 atcagtatcc cgaaaaattt ggtttttagt gaggaaagat ttaactgatc tatatcatct
      661 tggatgggca ttttatatta aaattccttg cccggcagga agaaaaaatt ttttggcgaa
      721 tctagtggta agaccacatt atattaagga cctaccatgc aaggtgagtg aatcaatgga
      781 tgaaagaaca ggaaaaaaag cctttcagga ggtggtggcc cgactggagg cgttgatcaa
      841 gaccgggcag gtcggtttcg gagagtatct cccccccgaa cgtgagctgg ccggcagatt
      901 tggtgtagga cgtcccgcgg tgcgcgaggc cttaaaggcg cttgaaattt tgggaatggt
      961 cgtcatcgag catgggaaag gggcccgggt cacaacgccc tccctagaga gttggttcca
     1021 agcgattggt acatcgctgg ttttatcccc ggcggatttt ttacacctga tggaggcacg
     1081 tgagactttg gaaccgcaat tggcctttat tgcggcttca cgggctaccg cagagcaatt
     1141 gcaggaactt ggctcgagtt tgaccgtgat gaaccgggcc ttggaaaagg ttactgaatt
     1201 cgcagccgcg gattaccgtt ttcacggttt gatcgcccaa gcgaccaaca atccggtgct
     1261 gatcaagatt tatgaagcca tagccgggtt gttatggctg ctccagcagg agacggcggt
     1321 ggttccggat cgccaaaaga gttatacgta tcatgaggcg atctacagag ccgtctttga
     1381 acggaatccc atggttgccc gggaagagat ggaattgcac ctaaaggata ccagggaaag
     1441 gtttttggat ttccggccag aatagactgg aacaacacca ttctatgggt tttttccaat
     1501 acttaacggg tggccccagc ccccaaaaag gagttaatgt agatgaaagt tgcagcttat
     1561 gaattgttca tggtcaaacc ccgctggctg tttttaagac ttgagaccga taccggcctg
     1621 gtcggttggg gtgagccggt tgtggaaggc cgggctgaaa cagtaaaagc cgctgttcag
     1681 gaattaatgg attacgtaat gggacaggat ccgtcgcgca tcgaggattt atggcaggtg
     1741 atgtacagag gcggttttta cagaggcggt cccatcctga ccagcgcgat ctccgggatt
     1801 gatcaagccc tctgggatat taaggggaaa tattacaatg ctccgatata tgagttactg
     1861 ggaggcgctg tacgggagcg ggttcgggtc tatacctgga tcgggggaga tcgccccgcc
     1921 gaggtaaagc aagaggctct acggaagttg caagccggtt ttaccgcggt taagatgaat
     1981 gccaccgaag agatgcaacg gatcgattct cctgcagcgg tggatgaggt attggcccgc
     2041 ctggcggcgg tgagagaggc agtgggaccg aaattcgaca tcggtctgga ctttcacggt
     2101 agagtctcaa agacaatggc gaaaatattg gcgcgggaga tggaacctta ccatcccttc
     2161 ttcatcgagg aaccggtctt ggctgaaaac aatgaggcgt tgcgggagat tgcctccgcc
     2221 accaccattc ccattgccac cggggaacgg cagtttaccc gttggggatt taaacaggtc
     2281 ctcatcgacg gacacgtgga tcttatccaa ccggacatct cccattgcgg gggaatttct
     2341 gaattgaaga aaatcgccgc tatggccgag acattcgacg tggctgtggc gccccattgc
     2401 ccgttgggac cgatcgcact tgccgcttcg ttgcagctcg acttttgcac ccccaatgtg
     2461 ttgatccagg agcaaagctt aggtattcat tataaccagg gaagtgatct cctggattat
     2521 ttggaggacc ggaccgtatt cgattaccgg gatggctatg tgagtcggat gaccggaccg
     2581 ggattgggga tccggatcga tgaggccaaa gtcagggagg ccgcccggga gggtcatcgt
     2641 tggcgtaatc cggtttggag gcacgccgac ggcacggttg ccgaatggta accatttggg
     2701 tttatgattc ttacatacaa aaagggttgg cttgggggcc aatccttttt tattacatac
     2761 gactatctgc aaatgtttaa acgaaatccg gtcaacgcgg catatagccc aatgccaggg
     2821 aaatcctttg acctacggag attaccgggg gggtgatctg atccagtttc tcccgggtca
     2881 ttctcagggt tgggccggaa atactgattg cggcaaccac agttccctga tggtt
//
LOCUS       DASVMB010000456         6893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_425859,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000456 DASVMB010000000
VERSION     DASVMB010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425859"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="lysS"
                     /locus_tag="VF531_12740"
     CDS             complement(<1..1324)
                     /gene="lysS"
                     /locus_tag="VF531_12740"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7714874.1"
                     /translation="MESEVEKVSEQMELRRQKLGKLREHGINPFGERYDRTHRLNQIT
                     DEFEALENQTVKIAGRLVAKRDQGKACFGNLNDQSGTLQIYAKIDVLGEEKYQELLDL
                     DLGDIIGITGKVFKTHRGQITVQVEDFRLLSKALRPLPDKWHGLKDTDLRYRQRYLDL
                     ISNPEVRETFLKRTQIVRSIRNFLDNRDFVEVETPILSSIAGGGHARPFNTHHNTLDL
                     DLTLRIALELYHKRLIVGGFDRVYEIGHCFRNEGISTRHNPEFTMLELYQSYADYQIM
                     MELVEGMISETARKVCGTTKLEYQGTAIDLTPPWPRLSIVEAIKQFTGIDWMTIDSDG
                     AAVAAAQSLKIKIDPKATKGMVLDEICSEYVEPKLIQPTFLVDYPIEISPLAKKIESN
                     PELTYRFELFIYGREMANAFSELNDPVDQRERFMEQAREKAKGNEEAMV"
     gene            complement(1423..1908)
                     /gene="greA"
                     /locus_tag="VF531_12745"
     CDS             complement(1423..1908)
                     /gene="greA"
                     /locus_tag="VF531_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7714875.1"
                     /translation="MGNEKDKEVILTLDGLSKLEKELEHLKTVRRREVAERIKQALEF
                     GDISENSEYEDAKNEQGFIEGRILSIEKMLRNVKIIDEQEVNNDAVSVGAKVTLIEVN
                     GGSEVEYMIVGSAEADPEQARISNESPVGRSLMGQKVGSVVNVNVPMGTIQYKIKAIK
                     K"
     gene            complement(2335..3243)
                     /locus_tag="VF531_12750"
     CDS             complement(2335..3243)
                     /locus_tag="VF531_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714876.1"
                     /translation="MGYFGFISTLLKVDSPWGKMLRFLPEKWRYYNGPLQSGEIAGVL
                     PGETVQGFGWSVPDSGMQVWQESRYIRLWERFLVDLDERDIKVIGLDASSGFRPPRRL
                     TGKTVFPGICDGRALELLLFFNRFRTLLRSMETPPQKTKVLIIWEEGNLGLACARLVA
                     REVRFLSLVHPNARYLERAAELIMAETGIATQIFTVPPDSVRSRIVIVCGPLHQYRLT
                     RPSRHYQWFELFQKNPSLTVLNADLPITALCRQERLPLYPALAEAILRSSFDVNPGVW
                     YGSELPLEHVVKMAELLREVGMKIAI"
     gene            complement(3299..4201)
                     /locus_tag="VF531_12755"
     CDS             complement(3299..4201)
                     /locus_tag="VF531_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HEX7714877.1"
                     /translation="MKRIVSVSLGSSQRDHRAEVEFFGEKVVIERIGTNGDLKRVISL
                     IQSLDGKVDAFGMGGIDLYLTADGRRFVIQDGARIARAAKISPILDGSGLKNTLERKV
                     VGYLQEELGIALSGKKVLMVSAVDRFGMAEAFDAAGCRMVFGDLIFALGIPIPITTLK
                     GLRKLAFTFLPVITRLPFTFLYPTGSKQDQITPKYTKYYEDADIIAGDFLYIRKYMPQ
                     NLKGKIIVTNTVTPANISELKERGASLLVTSTPEFNGRSFGTNVMEALLVSFSGKRPD
                     ELGEADYLQLLDKLHFQPRLVRLS"
     gene            complement(4272..5321)
                     /gene="dusB"
                     /locus_tag="VF531_12760"
     CDS             complement(4272..5321)
                     /gene="dusB"
                     /locus_tag="VF531_12760"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX7714878.1"
                     /translation="MLELGTLRLENPVVLAPMAGVTDPPFRRIVREYHTGLVCGEMVS
                     SMALHYNSRKTRELIQIAGEEHPVSIQIFGADPGIMAEAARVVADCGADIIDINMGCP
                     VPKIVKNGEGAALMRNLPLAAAIIGTVVKSVTLPVTVKFRLGWDKEHFTAAELARIAA
                     ENGAVAVTVHGRTREQFYQGEADWESIAAVKRSVAIPVIGNGDIDSPRAAAEIIRQTG
                     CDGVMIGQAALGRPWLFRQVSVYLETGTLVPDPPLAKQFETIRQHLRLQVAYAGEARG
                     IKEMRKHLAWYLKGTPGAARLREKLIALTSLSEVFRELGEYAGSLGVEWPDQQVDVSF
                     QTNTGAGFTVDSWNR"
     gene            complement(5322..6632)
                     /gene="hflX"
                     /locus_tag="VF531_12765"
     CDS             complement(5322..6632)
                     /gene="hflX"
                     /locus_tag="VF531_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7714879.1"
                     /translation="MPRFWGTRKDKYRIIMDTPRIERAILVGISPAGADHNQAQYQWL
                     EFSELARTAGAEVVGSITQERKSPDPAFFIGSGKVQWLAEEARRLEADLIITLQELSP
                     SQVRNMEEQTGVRVIDRTNLILDIFASRARTREGKLQVELAQLDYLLPRLGGSTRHLS
                     RLGGGIGTRGPGESKLEADRRRVRRRIAELRRQLLEVQNQRKVQRHRREKQQIPTVAL
                     IGYTNAGKSTLFNLLTAADVSAADRLFETLDPVSRRVRPPSGKEFILMDTVGFISNLP
                     HQLVAAFKATLEETTRATLLLHVMDGTAPNLAVQAEAVRTVLDEIGIATKERLNLLNK
                     IDLIDTDPTIERMAKEWEALPISARTGAGMEELLAVIFGKLADTTAIYRVKIPFGEAG
                     LVDRIHRQGRVLEEVFTPEGVCLRVETEPALADKYHQYFFREGS"
BASE COUNT         1622 a         1939 c         1671 g         1661 t
ORIGIN      
        1 agaccatggc ctcttcatta cccttggcct tttcccgggc ttgctccata aagcgttcac
       61 gctgatcgac ggggtcgttc agctccgaaa aggcattcgc catttcccgt ccgtaaatga
      121 ataattcaaa gcgataggtc agttccggat ttgactcaat cttcttggcg aggggcgaga
      181 tctcaatggg ataatcaacc aggaatgtgg gttgaataag tttcggttcc acatattccg
      241 aacagatctc gtcgagtacc atgcccttgg tggccttggg atctatctta attttcaagg
      301 attgggcggc agccacagca gccccgtcgg agtcgatggt catccagtca atcccggtaa
      361 attgtttgat tgcctcaaca atggatagtc tgggccaagg cggggtgaga tcgatcgccg
      421 tgccctggta ttccagctta gtggtaccgc aaaccttccg ggcggtttcg gagatcatcc
      481 cttccaccag ttccatcatt atctgataat ccgcgtagga ttgatataac tccaacatgg
      541 taaactcggg gttatggcgg gtagagatcc cctcattccg gaaacaatgg ccaatctcat
      601 aaacccggtc gaagccgccg acgattaagc gtttgtggta taactccagc gcaatccgga
      661 gggtcagatc caaatccagc gtgttatggt gggtgttaaa ggggcgggca tgtccacctc
      721 cggcaatgga gctgagaatc ggcgtttcca cctcaacaaa atcccggtta tcgaggaaat
      781 tccggatcga acggacgatc tgggttcgct tcaaaaaggt ctcacgcacc tcgggattgg
      841 atatcaaatc caggtaacgt tggcgatagc gaagatcggt atccttcaaa ccgtgccatt
      901 tatcgggaag cggccttagc gccttggaaa gcaggcggaa atcctcaact tgtaccgtga
      961 tctggccacg gtgggtttta aatacctttc ccgtgatgcc gatgatatcc cccagatcca
     1021 agtcaagtaa ctcctgatat ttctcctcgc ctaaaacatc gatcttggca tagatctgca
     1081 gggtaccgct ctgatcgttg aggttaccga aacaagcctt gccctggtca cgcttggcca
     1141 ccagcctgcc ggcaatcttc accgtctgat tctccagcgc ctcaaattca tcggtaatct
     1201 gattcaagcg atgggtccgg tcatacctct cgccaaaagg attaatccca tgttcccgca
     1261 gtttaccaag tttctgtcgg cgtaattcca tttgttcact tactttttca acttccgatt
     1321 ccaatgcgat cccctccatc gcaaaaaaac atcaccccga agagtgtaaa aggctgccca
     1381 aaaatatcac catatttttg ggcagcctga ctttttttgc ggttactttt taatcgcctt
     1441 aatcttatat tgaatcgtcc ccatcgggac attaacattg acaacgctac cgaccttctg
     1501 ccccatcaag ctccggccca ccggagactc attggagatc cgggcctgtt cgggatcggc
     1561 ttccgccgaa ccgacaatca tatattccac ctcggatccg ccgttcactt caataagcgt
     1621 gactttggcc ccgacactga cagcatcgtt attaacttct tgctcgtcaa tgatcttcac
     1681 gttccgcagc atcttctcga tggataaaat tcggccttcg ataaagcctt gttcattttt
     1741 ggcgtcctca tattcggagt tttcgctgat atccccaaat tccagggctt gcttaatccg
     1801 ttccgccact tcacgacggc gtacggtctt taaatgctca agctcctttt ccaatttgga
     1861 gagtccgtct agagttagga tgacttcttt gtccttttcg ttacccataa tcccgcccct
     1921 tccaggccga ttcttggcgc gcgttgcgcc gcgccattgc gctaattaat atatgttcac
     1981 cctaaataga ttattctgat accaggtggt taaaaggccg agatacagga gggttttcct
     2041 cccgtacgga tttaaaaaaa ttcgcccggc aagctacccg aagcgcgccc ggaacccggc
     2101 aaaatgccgg ttccttcggt gcggtaattc agataaagcc cccgaacaac ggtggaagga
     2161 ccctgttcaa atgcgtggtt attcagaaat gtgagcgcgc atgcgcgttc acggaaaata
     2221 tggcgtgccg gtcaataatg cggctactaa agagtaagca ccggatcctg gctccagtgc
     2281 cttaaagcat ttttattata atcgaaacat cattgggtgt caagcaccct cgcgctatat
     2341 ggcgatcttc atacctactt ccctaagaag ttccgccatt ttaaccacat gttccagggg
     2401 gagctcggac ccataccaaa ccccgggatt cacgtcaaag gaggagcgaa gaatcgcctc
     2461 ggccaatgcc ggatataaag gcaaccgttc ttgacggcaa agggccgtaa tgggcaggtc
     2521 cgcatttaaa accgtcaggg aaggattctt ttgaaaaagt tcaaaccatt ggtaatgacg
     2581 ggaggggcga gtcagccggt actgatgcaa cgggccgcag acaatgacga ttcgggatct
     2641 gacagaatcc gggggaacag taaagatctg cgttgcgatt ccggtttcgg ccatgattaa
     2701 ttctgcggcg cgctccagat atcgggcgtt cggatggacc agactcagaa accgtacctc
     2761 cctggctacc aatcgggcgc atgccagtcc caagttgcct tcttcccaaa tgatgagaac
     2821 cttcgtcttt tgcggcgggg tttccatact ccgcagcaag gtccggaaac ggttgaaaaa
     2881 caataacaac tccaaagctc ttccatcaca aatccccgga aaaacagtct tacccgtcaa
     2941 acggcgtgga ggccggaacc cgctggacgc atcaagacca attaccttga tgtcgcgctc
     3001 gtcgaggtca accagaaatc gttcccagag tcgaatataa cgcgattcct gccaaacctg
     3061 catgccacta tccggtacgc tccagccgaa tccctgtacc gtttcccctg gaagaacccc
     3121 cgcgatctcg ccgctctgta acgggccgtt ataataccgc cacttctcgg ggagaaaccg
     3181 taacatttta ccccagggag aatccacttt taataatgtc gagatgaacc cgaaataacc
     3241 catgcaaccg cctccatcat taatacttat gaagagtggt tgcgggttat ttatgatatt
     3301 atgataaccg gaccagtcgc ggttgaaaat gcagcttatc caatagttgc aagtaatcgg
     3361 cctctcccaa ttcatccggg cgtttaccgg agaacgaaac aagtaacgcc tccatgacgt
     3421 tcgttccaaa agaacggccg ttaaattcgg gagtggatgt caccaaaagt gaagcgccgc
     3481 gttcctttaa ttcgctgatg tttgccggag ttaccgtatt ggtgacaatg atcttgccct
     3541 tgagattttg gggcatatac ttccggatat agaggaaatc gccggcgata atatcggcgt
     3601 cttcataata cttggtgtat ttgggcgtta tttgatcctg tttggaaccg gtggggtaca
     3661 agaaggtaaa cggcaaccgg gtgatgaccg gcaaaaatgt aaatgccaat ttacgcaaac
     3721 ctttcaaggt ggtgatgggt attggaatcc cgagggcaaa aatgaggtcc ccgaagacca
     3781 tccggcatcc ggccgcgtca aaggcttcgg ccattccgaa tcgatcaacc gccgaaacca
     3841 ttagaacctt cttccccgaa agcgcaatcc ccaattcttc ttgaagataa ccaacgacct
     3901 tccgttccaa agtgttcttt aagccgctgc cgtcgaggat cggcgagatt ttggcggcgc
     3961 gggcgattcg ggccccgtcc tgaatcacaa agcgccgtcc gtctgcggta agatacagat
     4021 cgatcccgcc cattccaaag gcatctacct taccgtcgag cgattgaatc aaggagatta
     4081 ctcttttgag atcaccgttg gttccgatcc gttcaataac cactttttcc ccaaagaact
     4141 caacctcggc ccggtggtct ctttgggaac tccccaggct tacactgaca attcgtttca
     4201 tggatcgcac tcactttcgt cggtcgggct gagtttaccg agctccttgg caacccttgg
     4261 cctgtccatt ttcaccggtt ccaagaatcg acggtaaacc ccgccccagt attggtttga
     4321 aaagaaacat cgacttgctg atccggccac tcaactccaa gggatccggc atactcccct
     4381 aactccctga aaacttccga aagagaggtt aatgcaatca gcttctcccg taaacgggcc
     4441 gcgccggggg ttcccttgag ataccatgcc agatgcttgc gcatctcctt aataccccgt
     4501 gcttctccgg cataggccac ctgaagccgg agatgttggc gaatggtctc gaactgtttg
     4561 gcgagcgggg gatcaggtac caaagtgcct gtttcaagat aaacggagac ttgtctaaaa
     4621 agccacggcc tgcccaaagc ggcttggccg atcattaccc catcgcatcc ggtttgacgg
     4681 atgatctccg ccgcagcccg gggtgagtca atatccccgt taccgataac cggaatggcc
     4741 acactcctct tcactgcagc aatgctctcc caatcggcct caccctgata aaactgttcc
     4801 cgggtccggc catgaaccgt cactgccacc gcgccgttct ccgcggcaat ccgtgccagt
     4861 tcggccgcag tgaaatgctc cttgtcccac cctaaccgga atttaaccgt gaccggaagt
     4921 gtaaccgact tgaccactgt tccgataatc gccgcagcta agggaaggtt ccgcatcagg
     4981 gccgcgcctt ccccgttctt gacgatcttc ggcaccggac aacccatgtt gatatcgatg
     5041 atatccgcgc cacagtccgc cacaacccgg gccgcctctg ccataattcc gggatcggcg
     5101 ccgaagatct ggatgcttac cggatgctcc tcccccgcta tctgaatcaa ttccctggtc
     5161 tttctactgt tataatgaag ggccatggaa ctgaccatct ctccgcagac caaaccggta
     5221 tgatattccc ttacgatccg gcggaaaggt ggatcggtca ccccggccat cggtgccaaa
     5281 accaccggat tttccagcct cagtgtaccc aattcaagca tttaggaacc ttcccggaaa
     5341 aagtattgat gatatttatc ggccagagcc ggttcggtct cgacccggag acaaactccc
     5401 tccggagtga aaacctcctc cagcacccgg ccctgccggt gaattcgatc aaccagaccc
     5461 gcctcgccga agggaatctt gacccggtaa atagcggtgg tatcggccaa tttaccgaag
     5521 atcaccgcca gaagttcctc catacccgcg ccggttcggg ccgatatcgg caaggcttcc
     5581 cattccttgg ccatccgttc aatggtcgga tccgtgtcta tcaggtcaat cttgttcaaa
     5641 agatttaacc gttccttggt cgcgattcca atctcgtcaa gtacggtccg gacagcttcg
     5701 gcttgaacgg ccaggttggg agctgtgcca tccatcacat gtaacaataa tgtggcccgg
     5761 gtagtctctt ccagggtggc cttgaatgcc gccaccaatt gatggggcag attactgatg
     5821 aaacccaccg tatccataag aatgaactct ttaccgctgg gaggacggac ccggcgcgaa
     5881 accgggtcga gtgtttcaaa gaggcggtcg gccgcggaaa catcagccgc agtcagcagg
     5941 ttgaacaaag tggactttcc ggcgttcgta tatccgatca aagccacggt cggtatttgt
     6001 tgtttttctc tgcgatggcg ctggaccttc cgttgattct gtacttcaag caattggcgc
     6061 cgaagttcgg cgatgcgacg ccgcaccctg cggcggtcgg cctcaagctt cgattcgccc
     6121 gggccccggg ttccaatacc accgcccaag cgggagaggt ggcgggttga tccgccgagc
     6181 cggggcagta aatagtcaag ctgagccagt tccacctgga gtttcccctc acgggtacga
     6241 gcacgggagg caaagatatc caggatcaga ttggttcggt caatcacccg gaccccggtc
     6301 tgttcttcca tatttcttac ctgactgggg ctcaattctt gaagggtgat gatcaagtcc
     6361 gcctcgagcc gccgggcttc ctccgcgagc cactgaactt taccggaacc gataaagaat
     6421 gcggggtcag ggcttttacg ttcctgagta atacttccga caacctccgc cccggcggta
     6481 cgcgccagtt cggaaaattc aagccattgg tattgggctt gattgtgatc cgccccggcc
     6541 gggcttatgc cgaccaggat tgcccgttcg attcgcggtg tatccataat aatcctgtat
     6601 ttatccttcc gggttcccca aaagcggggc attttccttg tctgagagcc gtcaccggta
     6661 ttttccccgg ttaaccaaga taaaaaaact acccccatta tatcataggg ggcagtttct
     6721 ggaaagttat cgcgggccaa ggtcccgggc atcaaattct ttcattaaac aagttgtcag
     6781 gtgccgtcgg tcagattggg ggtggccatg acgatctcgg tgatattccg taccgggtca
     6841 aacctggttc caaattcaat attaatctgc tgaaagttat aagttacggc gtc
//
LOCUS       DASVMB010000457         6394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_352438,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000457 DASVMB010000000
VERSION     DASVMB010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352438"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF531_12770"
     CDS             complement(<1..597)
                     /locus_tag="VF531_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714880.1"
                     /translation="MLKINRLRVEINTVNGVYGIDSSFHSGLNFVSSPDNTCGKSSIL
                     AAIYYCLGFEQIIGGVSGIGSKVLTSVFKSYIDDNGKTWTVTESGAYLEITNGNETIT
                     IFRSALAQNRDNRLITIYHSNYASIADPKTQSDDMFVNVQNSATGEKGFHTYLEEFLH
                     LELPEVRTSDEHERKLYLQVIFASMFIEQKHGWSDIFSG"
     gene            complement(584..1069)
                     /locus_tag="VF531_12775"
     CDS             complement(584..1069)
                     /locus_tag="VF531_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714881.1"
                     /translation="MSDNPLLRGDIVFDAKPDAVPYNYRISYKVSQLCLIMRICGWQD
                     SCSLIKLHMISFALYTRENMDKLLKFAKNELTNPPIVRFDPAVNKALTFAIAYGFVTQ
                     QKSGNFKLTDRGRNFADQVKFVGDLMVSEISDLSELSKGLTESKIKELSEIWRGKNAE
                     D"
     gene            complement(1074..2027)
                     /locus_tag="VF531_12780"
     CDS             complement(1074..2027)
                     /locus_tag="VF531_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714882.1"
                     /translation="MRFIEGYMTGNAWEELCVQCYRIKYQNEHYTAIPAMQGGDAGIE
                     GFTMNGIVHQCYCPEREYSDDDLYKHQREKMTTDINKLISPDYATRLRRLGVPPIMEW
                     HFVIPEYKDSRIIEHAETKRTEVIAEKSKRPDVFEYIREDFKIIIKTAEDFTPEISRI
                     VRTNLTDIRLNLAIQHTGTPDWSQCDSEKVANICRKIKAVMHVDDDKNDDLNDVINMY
                     IEFYISGLEIMNSLHLNAPDFYEDIYKLEQSYKNEVKIRTRMHTNRALNQTLFDDILN
                     EFKSKLESDFSRVLTQASIGELKQDLIASWLADCSMEFRSV"
     gene            complement(2338..2623)
                     /locus_tag="VF531_12785"
                     /pseudo
     CDS             complement(2338..2623)
                     /locus_tag="VF531_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002738755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(2750..3160)
                     /locus_tag="VF531_12790"
     CDS             complement(2750..3160)
                     /locus_tag="VF531_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7714883.1"
                     /translation="MKQDTDTIGKRIKALREAKNLKPKDIKELIDISPGNLSLMEHDK
                     INPSAEALIKLTKFFKVTSDWILLGDQLNEEGEKVTGIDFNLAVPDREMAIYLAKIID
                     LWKNGDERIKTWVMVQLEKAFPEIAEEMKRKGQK"
     gene            3392..3598
                     /locus_tag="VF531_12795"
     CDS             3392..3598
                     /locus_tag="VF531_12795"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HEX7714884.1"
                     /translation="MTQTNSRKIHNLKQQIAAGRQRLQKLWDARGHTDSGGIPDAEGI
                     SDADILAASIELDDLMNRYQKLTS"
     gene            complement(3801..4670)
                     /locus_tag="VF531_12800"
     CDS             complement(3801..4670)
                     /locus_tag="VF531_12800"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX7714885.1"
                     /translation="MGDIKIGDGSEEGLYLLDYHVHTKRCGHASGEDREYVEAAIEKG
                     LKELGFADHIPRFYYPENAKVTERGMSPADLEEYVGAVQDLKAAYPEIRIKLGLEVDF
                     VPGWEEQALKLLEPYPWDYLLGSVHFISEWNYGYIGYEKEHTPREIFTAYFDKVAAMA
                     VSGCFDILAHIDLPRRFFPRLPEAEMEELYQVLAARLGKAGAVIELNTFGIRSARQEA
                     VGVFPEAYLLRLCRQYGVQVTLGSDAHVPKDVAADFESAVNELELVGYDRLVTYQSRR
                     PALVEIAPCRARQ"
     gene            complement(4808..>6394)
                     /gene="alaS"
                     /locus_tag="VF531_12805"
     CDS             complement(4808..>6394)
                     /gene="alaS"
                     /locus_tag="VF531_12805"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7714886.1"
                     /translation="SEIKTRQAHCEMVIKAEEEGFNRTLDKGIELFENIAAELGKTGD
                     KIIPGADAFKLYDTYGFPLDLTELMAEERGLTVDRDGFDREMERQKTQARQSGKFSLS
                     EQAKWETLKDQPHSRFKGYDELNCRTELCMLGEDSEYWHLVFEETPFYGESGGQVGDR
                     GRITVDGREFPVADTQKSNDRIVHLVKKSGEFPKTAGCFELIVDEERRQATACNHTAT
                     HLLQAALRQVLGSHLHQSGSLVTPERLRFDFTHFEKISAADLQKVETIVNQVIGRALP
                     VIKQEMSYQDAVSTGATALFDEKYGDRVRVIKVGAFSKELCGGTHVDNTAAIRVFRII
                     SESSVATGVRRIEAVTGDEALKLYNNERETLTEVGRILKTDSHQVVAKLNTLLADYDK
                     MEQELTKASHDQAAGELNQLFGAIQTVDTVRYITARVDGFSQELMREAVDKLRDKMGT
                     GVAVLGAEDEGKVRFVVGVTKDLTSKIQAGTLIKQIAAVAGGGGGGRPDLAQAGGTDP
                     AKLDEALRLGEKLIREQFSA"
BASE COUNT         1666 a         1656 c         1244 g         1828 t
ORIGIN      
        1 acctgaaaat atatctgacc aaccatgttt ttgttcgata aacatgcttg cgaagataac
       61 ctgaagatat aatttgcgct cgtgttcatc agaagtccgc acttcaggca attccaggtg
      121 cagaaactcc tctaaatatg tatgaaaccc tttttcacct gtagcagaat tctgaacatt
      181 gacaaacatg tcgtccgatt gcgtcttggg atcagcaatc gaagcgtaat ttgaatgata
      241 gatagttata agtctgttgt cccgattttg cgccaatgcg cttcgaaaga tggtaatagt
      301 ttcgtttcca tttgtaatct ctaagtatgc tccagattcg gtaacagtcc atgtcttacc
      361 gttatcatca atatacgatt taaagacaga tgttaagact ttgcttccaa taccgcttac
      421 cccaccgatg atctgctcaa atccaaggca atagtatatc gcagccagaa tagaactttt
      481 tccacatgtg ttatccgggc ttgaaacaaa gttcaaaccg ctatggaagg atgaatcaat
      541 accgtaaacg ccattcactg tattgatttc gacgcgaagc ctattaatct tcagcatttt
      601 tacccctcca gatctcactc aactctttga tcttactttc ggtgagtccc tttgatagtt
      661 ccgataaatc cgatatttcg gaaaccataa gatcacctac aaattttact tggtcggcga
      721 aatttcttcc tcgatccgta agcttgaaat tgcctgattt ctgttgtgtt acaaatccat
      781 acgcaatagc aaaagttaat gccttattaa ctgctgggtc aaatctcaca attggaggat
      841 tcgttaattc atttttcgca aacttcaaga gtttgtccat gttttctctc gtatacaatg
      901 caaatgatat catgtgaagt ttaattaagg agcaactatc ctgccaaccg cagattcgca
      961 ttattaggca aagctgagac actttatagc tgatacgata gttataaggt acagcatcag
     1021 gctttgcatc aaaaacaatg tctcctcgta gtaaagggtt atctgacata actttacacg
     1081 ctcctgaatt ccattgaaca atcagccaac caacttgcga tcaaatcttg cttaagttca
     1141 cctatggatg cttgcgtcaa tactcttgaa aaatcacttt caagcttgga tttaaattcg
     1201 tttaagatat catcgaagag ggtttgattt aatgccctgt tcgtatgcat tctagtcctt
     1261 attttcactt cattcttgta actttgttca agcttatata tatcttcata gaaatcagga
     1321 gcattgagat ggagagaatt catgatttcc aaaccactta tgtagaattc gatatacatg
     1381 ttgatgacat cgtttaaatc atcattttta tcatcatcaa catgcatgac agcttttatc
     1441 ttacgacaaa tattcgcgac tttttccgaa tcgcattgcg accaatccgg cgttccagtg
     1501 tgttgaatgg ctaaatttaa ccttatgtca gtaagattgg tacgaacgat tcggcttatt
     1561 tcgggcgtaa agtcctctgc tgtcttaatt atgattttaa aatcttcacg gatatattca
     1621 aacacatctg gtctttttga cttttctgct ataacttcgg ttcgctttgt ttccgcatgt
     1681 tcaataatcc tggaatcttt atattcagga ataacaaaat gccactccat aataggaggc
     1741 acacctaatc ttcgcagtct agttgcataa tcaggtgaga ttagcttatt aatatccgtt
     1801 gtcatctttt cacgttgatg cttatacaaa tcatcgtccg agtattctcg ttcagggcag
     1861 taacactgat ggacaatacc attcatggta aatccctcaa tgccagcatc tccaccttgc
     1921 atagcaggaa tcgcagtata atgttcattt tggtacttta ttcgataaca ttggacacac
     1981 aattcttccc atgcgttgcc tgtcatatat ccctcaataa agcgcataat gctcaactgc
     2041 gatccccctt tagtagtctt aacgatattg aatttgtagt atctggacat gttgtaactg
     2101 cggagatatc cgcaaagaat tcaacatgat cctccgtata atagactttg ataagctgaa
     2161 acatcagata aaccttaatc actgtgacct ccctttgcag gagatacctc ttatcgccta
     2221 tgctcggaag gtcttgaacg gtcacattaa ttctcctttc aattatcagt ttccattacc
     2281 atcttattcg gatcaacgcc ttatatcatt gtatggaggt taatcaacat cgttccacca
     2341 tccggcccgc attaacgcat caaccaaggc aatatatatt ctggttttcc ctgttccggt
     2401 tgccatctcc aacaaaactt gcgctgtttc ttctcaatag cctccatcac cgcgcgaatg
     2461 gcccgtatct gataatcgcg ctcggcaata cgggtgttaa tcaattcacc cgctaaaggt
     2521 ttacggtgat cgcggatgta ttggtatcgt tctaaatcat cttgcgttgg aaagccacta
     2581 ctttacggag cggataattc tctaaatccc aaaagtgaat ttcaagacta atgttttatt
     2641 ttacctacca aacggccaac tttgttagtt tatataaaac acttggtata taatctgtta
     2701 tccctactgc taatccgtta atctctatta agcataattc ggacagtgtt tatttctgcc
     2761 cttttctttt catttcctca gcaatctccg gaaacgcctt ctccaattgg accattaccc
     2821 atgttttaat gcgttcgtcc ccgttcttcc atagatcaat aatctttgcc aagtatattg
     2881 ccatttccct atcaggaacc gctaagttaa aatctatacc tgttactttc tctccctctt
     2941 cattaagctg atcgccaagc aatatccaat cggaagttac tttaaaaaac ttggtcaatt
     3001 ttattaaggc ttctgcagat ggattaatct tatcatgttc catcaaactc aagtttccgg
     3061 gagagatatc aattaattcc ttgatatcct tcggtttcaa attcttcgct tctcgtaagg
     3121 cctttattct ttttcctatt gtatccgtat cttgtttcaa gtttaaaatt acctcttgat
     3181 tatattttaa atttagaata taatgaaact ggccattaaa atccgaaaac ataaacagaa
     3241 aaaaacaaca taataccttc aagattacca atcatgaaag cattacaata aaattccagg
     3301 ctcaaaccat ccatttgcgc cctaattccc tattacctaa aatttcaatt attaccatta
     3361 tatcatttat tcttgataaa taaaaaggat catgacacaa accaactcaa ggaaaatcca
     3421 taacctaaaa caacaaatcg ccgccggacg gcagaggctt caaaaactct gggacgcccg
     3481 cggccatacc gactccggcg gcatccctga tgccgagggc atctccgatg ccgatatcct
     3541 agccgccagt attgagctgg acgacctgat gaaccggtac caaaaattaa cctcttgaat
     3601 tggtgcgtgc gcacacgaac tcaagaggtt acaaaaggaa caaagcagtc taataatgtt
     3661 ttagggatac gattccctcc cccatctgaa tgtgccctaa tgaataacga ctaaatgggg
     3721 gagggctgac ccggatgttg gctgaggagg atacacgcct cttgcctcac gtctcttaac
     3781 tgttgcctgt tgcctgttcc ctattgccgc gctctgcagg gcgcgatctc caccaacgcc
     3841 ggtctgcgac tttgataagt cacgagccgg tcatacccga ccagctccag ttcattgact
     3901 gcgctttcaa aatccgcagc cacatctttg gggacgtggg catcggaacc caaggtgact
     3961 tgcaccccgt attgcctgca aagacgaagc agatatgctt cgggaaagac ccccaccgcc
     4021 tcctggcggg cgctgcggat cccgaaggtg ttcaattcga tcaccgcccc ggctttcccc
     4081 aggcgggcag ccaatacttg atagagttcc tccatctccg cctcgggtaa ccgtggaaaa
     4141 aagcggcgcg gcaggtcgat atgagccagg atatcaaagc acccggaaac ggccatagcg
     4201 gctactttgt caaagtacgc cgtaaaaatc tcccgcgggg tgtgttcttt ttcatacccg
     4261 atataaccgt agttccattc ggagatgaaa tggaccgaac caagaaggta atcccatgga
     4321 tagggttcca gaagctttaa ggcttgctcc tcccatcccg gtacaaagtc gacttccagt
     4381 cctaatttaa tccgaatctc cggataggca gccttcaaat cctgtaccgc accgacgtac
     4441 tcctcaagat ctgccggact cattccgcgt tccgtaacct tggcattctc cggataataa
     4501 aatcggggaa tgtgatccgc aaaccccagc tcttttaagc ctttttcgat cgcggcctcg
     4561 acgtattccc ggtcttcgcc cgaggcatga ccgcaacgtt tggtatgcac atgataatcg
     4621 agcagataca agccttcttc actcccatcg ccaatcttga tgtcccccaa cccgaaaaag
     4681 ccggaaagcc tctcttcgcc ataaggtttc cctatccggt agaaaggcct tccccatatg
     4741 attgtggact tatgattcga atgattcgcc cgtccattga ttcaaagaaa gcgaaatcaa
     4801 aatccaatta cgctgaaaac tgttcccgga tcagtttttc cccgagtcgc agggcttcat
     4861 caagtttggc cggatccgtt ccaccggcct gagccagatc cggccggccg ccgccgccgc
     4921 cgcccgctac tgccgcaatc tgcttaatca aagttccggc ttgaatcttg gaggtcagat
     4981 ctttggtcac acccaccacg aatctgactt tgccttcatc ctccgctccc aatacggcaa
     5041 cgccggtgcc cattttgtca cggagtttat caaccgcttc ccgcatcagt tcctggctga
     5101 aaccatccac ccgggccgtg atataccgga ccgtgtctac ggtttggatc gctccgaaaa
     5161 gctgatttaa ttctccggca gcctgatcat ggcttgcctt ggtcaactct tgctccatct
     5221 tgtcataatc ggcgagcagg gtattcaatt tggccacaac ctgatgactg tcggtcttta
     5281 aaatccgtcc gacctccgtc aaggtttccc gttcgttgtt ataaagtttg agagcttcat
     5341 ccccggtaac agcctcgatc cgccgtacac ccgtggccac actgctttca ctgataatcc
     5401 tgaacacccg gattgctgcg gtattgtcca catgtgttcc gccgcataac tccttactaa
     5461 aagcgccgac cttgatcacc cggacccgat caccgtactt ctcgtcaaaa agcgccgtgg
     5521 cgccggtaga aaccgcatcc tgatacgaca tctcctgctt tataacggga agcgccctgc
     5581 caattacctg gttcacaatg gtttcgacct tttgaaggtc tgcggcgctg atcttttcaa
     5641 aatgagtgaa gtcaaaccgc aaccgctccg gggtgaccaa cgagcccgac tgatgcaaat
     5701 gagagcccag gacctgacgg agagcggcct ggagcaaatg ggtggccgta tggttgcaag
     5761 ccgtcgcctg acgccgttct tcatcgacaa taagctcaaa acagccggcc gtcttgggga
     5821 attcgccgga ctttttcacc agatgcacga tacggtcatt ggacttttgg gtatcggcga
     5881 cgggaaactc ccggccgtcc acggtgatcc ggccccgatc cccgacttgt ccaccggact
     5941 cgccgtaaaa aggcgtttcc tcgaagacca aatgccaata ttcggaatcc tcgccaagca
     6001 tgcaaagttc cgtacggcag tttagttcat cataaccctt gaaccgggaa tggggttgat
     6061 cttttagggt ttcccatttt gcctgttccg acagggaaaa ctttccgctc tggcgggctt
     6121 gagtcttttg gcgctccatc tcccggtcga aaccatcccg atccactgtc aagccgcgtt
     6181 cctcagccat gagttccgtc aagtccagcg ggaagccata ggtatcgtat agtttaaaag
     6241 catccgcgcc gggaattatc ttgtctccgg tcttcccgag ctctgcggcg atattctcga
     6301 agagctcaat tcctttgtcc agggtccggt tgaaaccttc ctcctccgcc ttgatcacca
     6361 tctcgcaatg agcttgcctg gttttgatct cgga
//
LOCUS       DASVMB010000458         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_131582,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000458 DASVMB010000000
VERSION     DASVMB010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131582"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2530)
                     /locus_tag="VF531_12810"
     CDS             complement(<1..>2530)
                     /locus_tag="VF531_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7714887.1"
                     /translation="KICGFGAALTVSGSEATLPEDQFSAYKAPEQTGRLNRNADFRTD
                     LYSLGIIFCRLLTGKLPFTPDDLLAELSFHLAKEALPINGLASEVPLPICKIVAKLTA
                     KNPDERYQNAESLIEDVKICLEQWRQGILATAATSEFLFSEQFKIPAEIYGREAELNS
                     LHEVLQRACRGGLHLILVSGDSGVGKTSLITEFKKSYHGTFFSTGKPDQYLPNTPYSM
                     FVQVLRGLVQIILSENEEGFARWQYKIKTAIGVNIQLLVELLPELGKVVAPQADLPSL
                     PLAEAQNRFNRTFLDFLQLFANSAQPLVIFFDDLQWAGKADLRLLGTVLASRLTHMMI
                     IGAYRSHEVSPGSPLESFLAAPEDHVPFDAIQLKPLSVTEADQFIRQTLNATPEASQG
                     LTAICLQKTGGNPFFLKQFLYAMQDQGLIRFNRLRRGWSWDEQEIGRTKITANVVDLL
                     VLKFEKLPPRVRLVLETAACIGNSFDIATLASVMGETPSVLRKTLQKAIEEALISSED
                     SGLERETFPERIPHYRFVHDRIQQTAYSLIPDILKRERHFLIGRTLWDRYGETVKDEQ
                     LYTITAHFNLAQKIITDETDRILLIKLNQTAAEKAKNTSAYEAELEYAVAGITAFQEV
                     WWQKYYHLGLSLHTQAAEAAAILGKFDLAELYADFGLAKVKTVIDGVELYRIKIEAYA
                     IQVDVKTLTTANDILKRLGVSFPEKPTWYHVRRSCRRLRRQLQHKSFRELSRLPILTD
                     PRQLAIIKIINTNTLHLYFLYSENPYLFFLQNFYALEIFLKHGNSPDAAMFYVIYAYF
                     LCGFDQDYRTGYQFGRLALEMAAANPDARSWEYKLKYYFNSSVLP"
BASE COUNT          673 a          575 c          614 g          668 t
ORIGIN      
        1 gggaagaacc gaggagttaa agtaatattt caatttatac tcccaacttc gggcgtcggg
       61 atttgcggcc gccatttcca gggccaggcg gccaaattga tatcctgtcc ggtagtcttg
      121 gtcgaacccg cataaaaaat aagcataaat cacataaaac atagccgcgt caggggaatt
      181 accgtgtttc aagaagatct ccaatgcgta aaaattttgg agaaaaaaca gataagggtt
      241 ttccgaataa agaaaataga gatggagtgt attggtgttg ataattttaa tgatcgcaag
      301 ctgccgcggg tctgttagta tgggcaatcg ggaaagttcc ctgaaacttt tatgctgcaa
      361 ttgccgccgt agtcgccggc aactgcgcct gacatgatac caggtgggct tttcgggaaa
      421 ggatacacct aatctcttca aaatgtcatt tgcggtggtg agggttttca catcaacctg
      481 tatagcataa gcttcgatct taatccggta aagctcgaca ccgtcgatca cggttttaac
      541 tttggccagt ccgaagtcgg catatagctc agctaaatca aatttcccta aaatcgcggc
      601 agcctcagcc gcttgcgtat ggagactcag tcccaggtgg taatattttt gccaccagac
      661 ttcctgaaaa gcggtaatac cggcaacagc atattccaac tccgcttcat aggccgaggt
      721 gtttttggct ttttcagccg cagtctgatt caatttgatc agcaatattc ggtccgtttc
      781 atcggtaata atcttttgag ccaggttaaa gtgggccgtg attgtataaa gctgttcatc
      841 tttaacggtt tcgccatatc gatcccaaag cgtccgtccg attaaaaaat gacgttccct
      901 tttcaggatg tcgggaatta atgaatacgc cgtttgctga atccgatcat gaacaaaccg
      961 gtagtggggt attcgttccg gaaaggtttc cctttccaaa ccggaatctt ccgatgatat
     1021 taaggcctcc tcaatcgctt tttgaagtgt tttccgtaaa actgaaggtg tttcgcccat
     1081 gactgaggct agtgtcgcga tgtcgaaaga gttgccgatg caggcggcgg tttctaggac
     1141 tagtctgacc cggggcggca gcttttcaaa ttttaagacc aatagatcaa ccacattagc
     1201 ggtgattttc gtcctgccga tttcctgttc atcccaagac cagccccggc gtaatcgatt
     1261 aaatcggata aggccttgat cctgcatggc atatagaaat tgtttcagaa aaaacgggtt
     1321 gccgccggtt ttttgaaggc aaatggcagt taatccttga ctcgcctcag gagtggcgtt
     1381 taaagtttgc cggatgaatt gatccgcctc ggtaacggat aatggcttta actggatggc
     1441 gtcaaatgga acatgatctt cgggagcagc caaaaagctc tccagagggc ttccgggtga
     1501 tacttcgtga ctccggtaag cgccgataat catcatgtgg gtcaaccggg aggccaaaac
     1561 ggtccccagc aatcggagat cggccttgcc ggcccactgg agatcgtcga aaaagatcac
     1621 taaaggctgg gcggagttgg cgaaaagctg gagaaagtct aagaaggtcc ggttaaaacg
     1681 gttttgagct tctgctagcg gaagcgacgg caagtccgct tgaggcgcaa cgacttttcc
     1741 aagttccgga agtaactcca ccaataattg aatgttaacg ccgatggccg ttttgatttt
     1801 atattgccag cgggcaaatc cttcctcatt ttcggataaa ataatttgga ccaatccccg
     1861 cagaacctga acgaacattg aataaggggt gtttggcagg tattggtccg gttttcccgt
     1921 gctaaaaaaa gtcccgtggt aagacttttt aaattcggta attaacgatg tttttcccac
     1981 tccggaatcg ccggaaacca ggattaaatg taacccaccc cggcaggccc gttgcaaaac
     2041 ctcatgtaac gaattcaatt ctgcttcccg gccataaatt tcagccggta tcttaaattg
     2101 ttccgaaaac aggaattcac ttgtcgctgc agttgcaagg ataccttgcc gccattgttc
     2161 aaggcagatt ttaacatcct caatgaggga ttcggcattt tgataccgtt catccggatt
     2221 ttttgccgtt aatttcgcca cgattttaca aatcgggagc ggaacctcgg aggccaaacc
     2281 attgatgggc agcgcttctt tagccaaatg aaaagagagt tcggctaaaa gatcatccgg
     2341 ggtaaagggt aattttccag tcaacaaccg acaaaaaatg attcctaatg aatataaatc
     2401 cgttcgaaag tcagcattcc ggttcaaccg gccggtctgc tccggggctt tatatgctga
     2461 gaattggtct tccggtagag ttgcttcgga accggaaacg gtgagtgccg caccgaaacc
     2521 gcagatttta
//
LOCUS       DASVMB010000459         9135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_352560,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000459 DASVMB010000000
VERSION     DASVMB010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352560"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF531_12815"
     CDS             complement(<1..262)
                     /locus_tag="VF531_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714888.1"
                     /translation="MKQSYINALYQSLVLERDYEPLQAEPGSNPFPEGILILHRYSRY
                     QYYLVQLLDGDRFANAAIREKLESDRLWLRTAPEEGRVHLITV"
     gene            544..1353
                     /locus_tag="VF531_12820"
     CDS             544..1353
                     /locus_tag="VF531_12820"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7714889.1"
                     /translation="MNWLDKLERKLGRFAIRNLMIYIIGLNGLVYLLMMLDRTGTLAF
                     QLRLIPELVFRGEVWRLVSYIFIPPATSPLWIVFILYFYYFVGNTLEQEWGSFRFNIY
                     YLLGIIATTVAAMITGGDATAVYLNLSLFLAFAYLYPDHQVLLFFILPIKVKYLGWFN
                     WVWLGYTVITGDIPVRAAAVAAVINFIIFFGPEIIAHLRVGRRSYYNRQRFSGNDRSD
                     FTIHRCCICGKTEKTDPKMDFRYCAQCEGDYEYCMEHLKTHQHKGKENLIQ"
     gene            complement(1566..3425)
                     /gene="ftsH"
                     /locus_tag="VF531_12825"
     CDS             complement(1566..3425)
                     /gene="ftsH"
                     /locus_tag="VF531_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7714890.1"
                     /translation="MFKVAREILLYFVVAIITLFIVAKFMDIKDPVESMNFDQFMKRA
                     ETGKIITAVINNNDGTISGKEQPYKGYQKYRTEGPANHPELFYDKLRQKGIKVSFSNA
                     GNGWWAVILPNLGFLVLFIIFWFFLLNQMQNSGNKAMSFGKSRARLHTEEHTKTTFED
                     VAGEDEAKEELVEIVEFLRQPRRFTELGAKIPKGVLLVGSPGTGKTLLAKAVAGEAGV
                     PFFSISGSDFVEMFVGVGASRVRDLFEQAKKNSPCIVFVDEIDAVGRQRGAGLGGGHD
                     EREQTLNQLLVEMDGFEPNSGIIILAATNRPDVLDPALLRPGRFDRQVTLDIPDIKGR
                     EDILKVHSKGKPLSSEVDLTVLARQTPMFTGADLANVLNEAALLAARRGKKRINMEEC
                     EDAIERVIAGPEKKSRVMNAKEKELTAFHEAGHALVGHYLPTVDPIHKISIIPRGQAG
                     GYTLALPLEDKHYNTRTELLENIVFALGGRAAEVVALGEISTGARSDLERATKTVRKM
                     VTEYGMSENLGYMTFGHGHEEQIFLGRDIARDKNYSEEIAAAIDREIKRIMDDSFVKA
                     TEVIKDHRELLDKLAHALLEKETIEGKEFLALMGETHEEQPNPTSDQEPEKQA"
     gene            complement(3643..5055)
                     /gene="tilS"
                     /locus_tag="VF531_12830"
     CDS             complement(3643..5055)
                     /gene="tilS"
                     /locus_tag="VF531_12830"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7714891.1"
                     /translation="MKEKVQATITRYHLLDPGERVVVGFSGGIDSVCLLHLLHSLTDL
                     RLDLWALYINHSLRPAENRNEEELLRQVGENWGVHTREIVIDIPARLREKPQSMELLA
                     REERYRIFRSFLKEIGASKVALGHHRDDQAETVLYRIIRGTGIDGLAGIPVCRDGIFI
                     RPLLAVTRNEIREYAAVHDLRWQEDSSNRQLVYRRNRIRHQLIPEIAADFNPGFKDAL
                     VRLSELAAEQRDFMETILSEYGERVMVREPGRIGLRMEPFTSLHPYLQYGLLKRLVEQ
                     VRPGYRVEIQPLRRLREKLQREGLRFRRMQLPKYILVYREREILFLEDDSESPNGFGP
                     VPLNTPGTTLVSELNFEFRATRESPPSSWKGVGFDETYLELGPENLPLKLRSWQPGDA
                     FRPLGTVGSQKLHDFFINNKIPRRIRKRIPLLVTADGRIAWVAGWRPSEEFKVRSSTA
                     EVWHITITGPDNALRGHPGD"
     gene            5477..5650
                     /locus_tag="VF531_12835"
     CDS             5477..5650
                     /locus_tag="VF531_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714892.1"
                     /translation="MSLYRDEFRKTAVRLRLIRDELLEKGFSETPKALAEAITYLESA
                     DKVYIRELAEKKN"
     gene            complement(5801..8260)
                     /gene="spoIIE"
                     /locus_tag="VF531_12840"
     CDS             complement(5801..8260)
                     /gene="spoIIE"
                     /locus_tag="VF531_12840"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HEX7714893.1"
                     /translation="MSGQAAVEERACIHANSGSRNQVHFFNYTKPQALQARSHACTIR
                     SFWSRLFEYGTDFCCAVLLSRFFIPGGLYPAGVGFFLASISRGHRFQWYESLLILAGV
                     GLGTYSVKGLAAACGISGCLFCLWSIAGCVNHFKKNSPALLFILACWAAIRFGLVAAF
                     NPGWNTWIWTGSEFLGTAVLGFVFQKGIGFLENPLQKDSGQAILALALLTVLALGGTR
                     GLVVYSINLTEIGSLLLLLIASYLGGGGAGAAMGIVIALILGVTAGNLFTLVATYGIS
                     GFLGGLFKNWGKWGTILGTGLGLGIIALQLHLDPAVMNQLLPWGIAMASFLVIPKSSL
                     SQVSSYFPNENKAEEPESQRQIKEVIYGRLNDLAEIFAQIARSFYEEQQAQAARHKMD
                     LYSLLDEVCTKNCQHCNGYEGCWGENFYATYREIFDLIAYAELYGEVGAKHLKGRLAK
                     SCFQQFKLLASINQLFERCQAEYTWQRKLDESKVFVAEQLQGVSDIISNLAGEVTTDT
                     SFKYEIEEKLRAGLNRVGMHIKELSVMTVRNQELEIRIRQHGCNQKRECVYLAGTMIS
                     RLLNQDYSVWEKNCQLENGDCSYCLVPTCSYQVRTTVCKLPKAGNESSGDTHTLQEMK
                     DGHFLAILSDGMGQGNTAAAESNIAVSLLNKLLASGIDREFAVKLINSVLLLRSPHES
                     FATVDLALIDLYTAQAEFIKIGAAATYVKRGKEVWSIKSTSLPAGILNDVDMERTIVQ
                     LQPGDMIIMATDGVVDSKAMVEGKEEWMVRALRQVEVVGPESLGEYLLSLAKINQDGI
                     PQDDMTVIVLQVMEKGNINLV"
     gene            complement(8506..>9135)
                     /locus_tag="VF531_12845"
     CDS             complement(8506..>9135)
                     /locus_tag="VF531_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714894.1"
                     /translation="NVVIVPCMFLGCLVVAIRAILSGSMDGRPDSANGYGLLAAIQFS
                     SYNLVLSMPVLLSLGKHYPRVRVLAGSGWVGSLGLGIMTALINWAILSHWPEAALVPL
                     PMAELAKSSGLGLYWGYAFILWAEMLSTLIADVYGVTERLQIILGWPFGRLAAVVAIS
                     GILIGQIGFSNLITYCYPFFGCVCLIVLLLILCKPRPSRNNGLTGGARK"
BASE COUNT         2386 a         2418 c         2044 g         2287 t
ORIGIN      
        1 agaccgtaat taaatgtacc cgcccttcct ccggggccgt gcgaagccat aaacgatccg
       61 attccagctt ttcccggatc gcggcgttgg caaacctgtc cccatcgagc agttgcacca
      121 aataatattg gtaacgagaa tagcgatgta aaataaggat cccttccggg aacggattcg
      181 accccggttc ggcctgcagc ggttcataat cccgttccag gaccagactc tgatacagtg
      241 catttatata gctttgtttc atctcttcac ctcgaaggca atagtacgaa agagtattaa
      301 ttgaagtcat atgggaaaaa ttgtctatcg gacggaatgg caaaatcaag ttgcttcatt
      361 tatctcacgc cttgtgccta cgtcttttgc ctgttgccaa ttgcctgttt cctgttgcct
      421 attataccat gaaccgcaaa ccattcccag gtccggtttt ttttgatatg ataaagaaag
      481 atccatttat ccggaaccgc cgcaaaacgg catataaagc aaaaacttca gaggagactg
      541 acaatgaact ggttggataa gctggaacgg aaattgggac gcttcgccat tcggaacctg
      601 atgatctaca tcatcggcct gaacggtttg gtttatttgt taatgatgtt ggatcggacc
      661 ggtaccttgg cctttcagtt aagacttatt ccggaattgg tcttccgggg tgaagtttgg
      721 cggctggtga gctatatctt tatccctcct gcaacctccc ccttatggat cgtctttatt
      781 ctttattttt attattttgt tgggaatacc cttgaacaag agtggggcag ttttcgtttc
      841 aacatctatt atctgctggg tataatcgca actaccgtgg ctgccatgat tacgggcggc
      901 gacgccacgg ccgtctacct aaacctctcg ctatttttgg cctttgccta cctctacccc
      961 gaccatcagg tgctgctctt ctttatcctg ccgattaagg ttaaatatct gggctggttc
     1021 aactgggttt ggttgggtta cacagtgatt accggggata tacccgtgag ggcggcggct
     1081 gttgcggcag ttatcaactt catcatcttt tttggtcctg agatcatcgc tcatttgcgg
     1141 gtgggccgcc gttcctatta taaccggcaa cgtttttccg ggaacgaccg gtccgatttc
     1201 accatccaca gatgctgcat ctgcgggaaa accgaaaaga ccgaccctaa gatggacttc
     1261 cgctattgcg cccagtgcga aggtgattat gaatactgca tggagcatct aaaaacccat
     1321 caacataagg gaaaagagaa cctgattcaa tagaatgttt caaaagggta aggagtaaac
     1381 tgtgcattac tgcttaaagt aagtctttcg agcattcata tttgaaaaat tcccggggtt
     1441 aagagagagg tcagatttta ggcccaaaag cgaccctagt gaagcataat caaaacaggc
     1501 aaatgaatca aaaaaagagc tgccattcag gcaactcctt tttggtatta tttaataccg
     1561 cgtatttaag cctgtttctc cggttcttgg tcggatgtcg ggttaggctg ttcttcatgg
     1621 gtttctccca tcaacgctaa gaactcctta ccctcgatgg tttccttttc aagcagcgca
     1681 tgggccagtt tatccaggag ctcgcggtga tccttgatga cctccgtggc cttgacaaag
     1741 gaatcatcca taatccgttt gatctcccgg tcaatggcgg cagcaatctc ctcactgtaa
     1801 ttcttgtcac gggcgatatc ccggcccaaa aagatctgtt cctcgtgacc gtgaccgaag
     1861 gtcatatatc ccagattctc actcattccg tattcggtga ccatcttgcg taccgtcttg
     1921 gtggcccgtt ccaagtcact ccgggcaccg gtgctgattt cacccagagc aacgacttca
     1981 gcggcgcgtc ctcccaaagc gaagacgata ttctccaaaa gttcggtccg agtgttataa
     2041 tgcttatcct caagcggcag tgccagtgta taaccgcccg cctggccgcg gggaatgatc
     2101 gaaatcttgt gaatcggatc gacggttggc agataatgcc caaccaaggc atggcccgcc
     2161 tcgtggaagg cggttaactc cttctccttg gcgttcatga cccggctctt cttctcgggg
     2221 ccggcgatca cccgctcgat ggcgtcttca cactcttcca tattgatccg ttttttgccg
     2281 cggcgcgccg ccagaagggc ggcctcattc aggacattgg ccagatcagc gccggtaaac
     2341 atcggcgtct ggcgggccaa aaccgtcaga tccacctctg aagagagcgg cttacccttg
     2401 gagtgaacct tgagaatatc ttccctgccc ttaatatccg gaatatccaa agttacctga
     2461 cggtcgaaac gccccggccg gagcagcgcc ggatcaagca catcgggacg attggttgcc
     2521 gctagaatga tgatgcccga attgggctca aatccgtcca tctctaccag aagctgattc
     2581 aacgtttgtt cgcgttcatc atgaccgcca cccaaaccgg cgccacgttg acggccaact
     2641 gcatcgatct catccacaaa gacaatgcaa ggtgaattct ttttggcctg ttcgaagagg
     2701 tcccgcaccc gcgaggctcc cacaccaacg aacatctcta caaaatccga accactgatg
     2761 ctaaagaaag gaacacccgc ttctccagcc accgcttttg caagtaaggt tttacctgtt
     2821 ccaggcgagc cgactaacag aacacccttg ggaatcttgg ctcccaactc cgtaaagcgg
     2881 cgcggttgac ggagaaattc gacaatctcc accaattctt ctttggcctc gtcctcaccg
     2941 gccacatcct caaaggtggt cttggtgtgt tcttcggtat gcagccgggc ccggcttttc
     3001 ccgaaggaca tcgccttatt gccgctgttt tgcatctggt tgagcaggaa gaaccagaag
     3061 atgatgaaga gaaccaaaaa gccgagattc gggaggatca ccgcccacca gccgtttccg
     3121 gcgttggaga aggagacttt gatccctttt tgacgtagct tatcgtaaaa gagttccggg
     3181 tgattcgccg ggccctcggt ccgatacttc tgatagcctt tataaggttg ctctttcccc
     3241 gagatagtac cgtcgttgtt attgatgacc gccgtaatga tcttaccggt ttccgcccgc
     3301 ttcatgaatt ggtcgaaatt catggactcc accgggtctt tgatatccat aaactttgct
     3361 acaataaaca gagtgatgat ggcgacaaca aaataaagta aaatttcgcg agcgacttta
     3421 aacaagcaga aacctccctt cggcaaatga agccgatttt gccatgtcaa atattatacc
     3481 atagtaaaaa tatgaataca acagatggga accttgccgg gtgtgatgtt gtaagccccg
     3541 gaagatcgac gcctcccttt tatctaaacc ggcactttag aggaagtcca aaaaaccgtt
     3601 aacctcttcc aatgccgaac tgtttccata ttggtaaaat ctctaatctc caggatggcc
     3661 ccttaaggca ttatcgggac cggtgattgt aatatgccaa acttctgcag ttgatgagcg
     3721 gaccttaaac tcctcgcttg gtcgccaccc ggccacccag gcaatccggc catcagccgt
     3781 aaccaagagc gggattcgtt tccgaatccg gcggggaatc ttattattaa tgaaaaaatc
     3841 atgcaacttc tgagaaccca cagttccaag ggggcggaat gcatctcccg gttgccagga
     3901 acgtaactta agcggcagat tctccgggcc aagttcgaga taggtctcat caaagcctac
     3961 ccctttccag ctggaagggg gggattcccg ggttgcccgg aattcaaagt tcaattcgga
     4021 taccaacgtg gttccgggag tatttaaagg taccgggcca aaaccgtttg gtgattcgga
     4081 atcatcctcc agaaaaagaa tttcccgctc ccggtagacc aaaatgtact taggcaattg
     4141 catgcggcgg aaacggagtc cctccctttg cagcttctcc cgtaatcttc gcaacggctg
     4201 aatctccact ctgtaaccgg gtcgtacttg ctcgaccaat cgttttaaga ggccgtattg
     4261 caaataggga tgaagagatg tgaagggttc catcctcaag ccgatccgcc ccggttcccg
     4321 gaccattacc cgttccccat attcgctcaa gatcgtttcc ataaaatcgc gttgttcggc
     4381 ggcaagctca ctcaaacgca ccaaggcgtc tttaaaacca ggattaaaat cggctgcaat
     4441 ctcaggaatg agttgatggc ggatacggtt tctccgataa accagttgcc ggttggagga
     4501 atcctcctgc caacgaagat catggacggc cgcatattcc cgaatctcat tccgggtgac
     4561 tgcaagtaaa ggccggatga agatcccgtc acgacatact ggaattcctg ccaaaccatc
     4621 aattccggtt ccccggatga ttcgatacaa caccgtctct gcctgatcat cacggtgatg
     4681 gcccaaggca accttagaag ctccaatttc cttcaaaaaa gatcgaaaga tccggtagcg
     4741 ctcctccctg gccagtaact ccatagattg cggcttttcc cgcaatcggg ccggaatatc
     4801 gatgacaatc tcccgggtat gcactcccca attctccccg acctggcgca gcagctcttc
     4861 ctcattacgg ttctccgccg gtcgtaagga atgattgatg tataacgccc atagatcgag
     4921 ccgtaaatcg gtgagagaat gcaacaagtg gagcaggcag accgaatcga tccctccgga
     4981 aaaccctacc acgactcgtt ctcccggatc aagcaagtga tatctggtaa ttgttgcctg
     5041 aaccttttct ttcaagggac cgctcccaaa attaaaattt tcccggcatc ctttctgatc
     5101 cgctataact ggcagctttc ccaaacccgg ctcgcgaagt ttaactgcgt tcccggcaaa
     5161 accgaatttc gcgtggactt caaagttaaa aaacgaatca ccggattaat agttctctta
     5221 acagattaaa attcctgtta taacttaaaa actactatca cttgcttaac gacttccttc
     5281 cgttatagtt cccaccggtt gaggattaat ccggtaagtt atggcaggta tactccgatg
     5341 ccttttcatc cggggttata tgcctgtcac tagagaaaac ttacagcaaa tcgctaaagt
     5401 ccggaaccct tcattccgaa cattagaaag gaaatagccg caatatacct taggtggaaa
     5461 gatgggatga atcttgatgt ccttgtatcg cgatgaattt cgtaaaacag ctgttcgtct
     5521 acggctgatc cgcgatgaac ttcttgaaaa agggtttagc gaaactccaa aggcactggc
     5581 cgaggcgatt acatatttgg aaagtgccga caaggtttat attcgggaac ttgccgagaa
     5641 aaaaaattag ttttgatctc cggaggaggc catactttta tccaatttac agaaacgcaa
     5701 aaggagtgaa acgcataagc gcttcacccc ataacggcat cgtcttttgg gagacggttt
     5761 acaataatat catgatcaca gggaccttgt catcggggct tcaaaccaaa tttatattcc
     5821 ctttctccat tacctgaagt acgatgactg tcatgtcatc ctgcggaata ccgtcttgat
     5881 taatcttggc cagactcaag aggtattccc ccaaagactc ggggccaact acctctacct
     5941 ggcgtaaagc tcttaccatc cattcttctt ttccttccac catcgcttta ctatccacca
     6001 ctccgtcagt ggccataata atcatgtctc cgggttgaag ctggactatg gttcgctcca
     6061 tatctacgtc attcaggatc cccgcgggca acgatgttga tttgatgctc cagacttcct
     6121 tacctcgttt cacataggtg gcagcggcgc cgatcttgat aaactcagcc tgtgcggtat
     6181 aaagatcgat caaagccaga tccaccgtag caaaagactc atgaggagag cgcagtaaca
     6241 aaacggagtt aatcaacttc accgcgaatt cacgatcaat tccgctcgct aaaagtttat
     6301 tcaagaggga taccgcaata ttgctttctg cggcggcagt gttcccctgc cccataccgt
     6361 cgcttaaaat agctaaaaaa tgtccgtcct tcatctcttg gagggtatgg gtgtctcctg
     6421 aagattcgtt accggccttg ggtagcttgc aaacagtagt acgaacctga tagctgcacg
     6481 ttggaaccag acaatatgag cagtctccat tttccagctg acaatttttc tcccataccg
     6541 aataatcctg attgagcagc cggctgatca ttgttcctgc caaatagacg cattcccgtt
     6601 tctggttgca accatgttgt cgaatccgga tctccagttc ctgatttcgg actgtcatga
     6661 cggaaagttc tttgatatgc attccgaccc gattcaatcc ggcgcgtagt ttctcctcta
     6721 tttcatattt aaaagaggta tcagtggtga cttcccccgc caggttactg ataatgtccg
     6781 aaacaccctg aagctgttcg gcaacgaaaa ccttgctctc atcgagctta cgttgccaag
     6841 tatattccgc ctgacacctt tcgaaaagtt gattaatcga agcaagcaat ttaaactgtt
     6901 gaaaacaaga cttggccaaa cggcctttga gatgtttggc gccaacctcc ccataaagct
     6961 cggcataagc gatcaaatca aagatctccc ggtaggtggc ataaaagttc tcaccccagc
     7021 atccctcata cccattgcaa tgctgacaat tttttgtaca gacctcgtcc agaagagaat
     7081 aaagatccat tttatgcctg gccgcctggg cttgctgttc ctcatagaaa ctcctggcaa
     7141 tttgggcgaa gatctctgcg agatcattca agcggccata tataacttcc tttatttgac
     7201 gctggctttc gggttcctct gccttgttct cgttcgggaa gtagctggat acttgcgata
     7261 aactactttt ggggatgacc agaaatgaag ccatcgcaat gccccatggt aaaagttgat
     7321 tcattactgc cgggtcgaga tgcagctgta atgcaatgat gcctaatccg aggcctgtgc
     7381 cgagaatggt tccccatttg ccccagtttt taaagagccc tcccaaaaag ccggaaatgc
     7441 cgtaggtggc taccaaggta aacaaattgc ctgcggtaac tcccaggatt aacgcgatga
     7501 ctatgcccat tgccgccccc gctcctcccc ctcctaaata tgaggcaatc agcaataata
     7561 agagcgaccc gatctcggtc aggttgatgg aataaaccac taacccccgc gttccgccca
     7621 atgccaaaac agtcaacaac gctagtgcga gtatggcttg tccagaatct ttttgcagcg
     7681 ggttttctaa aaaaccgatt cctttttgaa aaacaaaccc cagtacagct gtacccaaaa
     7741 actcagaccc agtccagatc caagtgttcc aaccggggtt gaacgcggca accagtccga
     7801 agcgaatcgc cgcccaacag gccaagatga agagaagtgc gggactattt tttttaaaat
     7861 gatttacaca tcccgcgatc gaccataaac aaaatagaca gccactaatt ccgcaagctg
     7921 ccgcgagccc ttttactgaa taagtcccca accccacacc ggctaagatc agcagactct
     7981 cataccattg gaaacgatgc ccccggctga tactggccag aaaaaatccg actccggcgg
     8041 ggtagagacc cccggggata aaaaaccgac ttaataagac ggcgcaacaa aaatccgtac
     8101 cgtattcgaa caggcggctc cagaaactac ggattgtgca tgcgtgcgac cgggcctgta
     8161 aagcttgtgg tttggtgtag ttgaaaaaat gcacctgatt ccggctgcct gaattggcat
     8221 gaatgcacgc gcgctcctca actgctgctt gtccggacaa tcatttcacc tcaccccgtg
     8281 gcatatattg ccaatattgc tcacgcggta aagtatacca tgaaggtcta ttgtgactat
     8341 gtcgtttttt caatgcaaat gatatatatt ttccttagtt ctccagtaaa atgcggcaga
     8401 tacgggggtt caaaagcggt atcccttgga aaatcatcac tttgccgtaa cgatttttaa
     8461 aacccatgat ctttaaaaac agacataaac cttaaatatc ggtccttatt tcctggcgcc
     8521 gccggtcaaa ccgttattcc gagacggcct tggtttgcag aggatcaata acagaacaat
     8581 caggcacaca caaccaaaaa acgggtaaca atatgttatc agattactga acccgatctg
     8641 cccgatgaga ataccggaga tggcaaccac cgccgccaaa cgaccaaaag gccatccaag
     8701 gatgatctgc agtctttcgg taaccccgta aacatccgca attagggtgg acaacatctc
     8761 cgcccataat atgaacgcat acccccagta taggcccaat cccgacgatt tggcgagctc
     8821 ggccattggt aacgggacta aagccgcctc cggccaatgg ctaagaattg cccagtttat
     8881 taaggcagtc attatgccca aaccgaggct tcccacccag ccgcttccgg ctaaaacccg
     8941 gacccgggga taatgtttgc ccagagaaag caagaccggc attgataaaa ctagattata
     9001 ggatgaaaat tgaattgccg cgagtaaccc gtatccattt gcagagtccg gcctgccgtc
     9061 catggaaccg gatagaattg cccggatcgc aacgaccaga cagccgagga acatacaggg
     9121 tacaatcacc acatt
//
LOCUS       DASVMB010000460         6010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_647301,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000460 DASVMB010000000
VERSION     DASVMB010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647301"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VF531_12850"
     CDS             <1..652
                     /locus_tag="VF531_12850"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEX7714895.1"
                     /translation="EGEWVTPTGAALIKTFAKSCSALPLLKISRVGYGAGTAERPVPN
                     VLRLILGENSRPTDQTDSQVVLETNIDDMNPEFYGRLGELLLENGAKDYFLTPVQMKK
                     NRPGVLVTVITPPERVNQLETLLFKETTTLGIRNYIVHRNCLERGELEIQISGIPIRV
                     KTAIREGEVFKFAPEYEDCLRTAGILQLPVKEIYDRAMFQARKILQAAGASSSGVE"
     gene            810..1310
                     /locus_tag="VF531_12855"
     CDS             810..1310
                     /locus_tag="VF531_12855"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX7714896.1"
                     /translation="MDIAAMGAWFAANGLLLSCFNVIIVLLVLLYFIIANARLRKYQM
                     LLKTSSGKDLEQMLLDLVEKTDLVYAKLTDFETKFESNRIVEAKHLQKIGLIRFKAFQ
                     NSGGDQSFALAMLDAAGDGIVISSIFGRDEARVYCKPVEKGDSSYPMSEEEKEAIVKA
                     LDLKKK"
     gene            1544..2494
                     /locus_tag="VF531_12860"
     CDS             1544..2494
                     /locus_tag="VF531_12860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7714897.1"
                     /translation="MMKTIKKRFGKGFTFLIVPNTGGAVKSYAIPFSLALLIIGIIGF
                     NLYIFVGYTTQIGQIQEFRTKIKTRDKKIDSLLSEQREVRPTLQKSYQMVEDLSELKQ
                     ERSRILSTWRNVLQKGGRSTTTSRGTIIRNTSYNLSTRPRVENETKSELADLSYNMNQ
                     VEGFIKEEAQSQHQLWVDLLAYERRLDHTPSIWPVRSFITSLFGFRLHPTLGYYKEHT
                     GIDLQARYGSRVAAAADGRVAFSGYKSGYGYTVILDHEYGYQTLYGHNSKLLVRVGQV
                     IKKGQIISLSGNSGTSTGPHLHYEVHVNNHAVNPSSFLRN"
     gene            2513..2929
                     /locus_tag="VF531_12865"
     CDS             2513..2929
                     /locus_tag="VF531_12865"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7714898.1"
                     /translation="MCAKERDGFNTRKVDTLIGKETSVKGNLDAPGGAVRIDGYYEGE
                     LHIGGDLIVGETGRVIGNIVAKNVVIAGEVKGSIESRGRLELAPTAKVIGDSKMVTLV
                     IEDGAFLQGMCAPLPRGDLKERGKALLVDTPEPEKA"
     gene            3122..3385
                     /locus_tag="VF531_12870"
     CDS             3122..3385
                     /locus_tag="VF531_12870"
                     /inference="COORDINATES: protein motif:HMM:NF015643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HEX7714899.1"
                     /translation="MQRTVALESGIQVLKPYLKDAGYRVVDLGGNPQKQNLDAVDAVI
                     ISGLDENLTGDSTRVTPGWVLNARGRQPEEILYDLEKHFRLMD"
     gene            complement(3389..4720)
                     /gene="glnA"
                     /locus_tag="VF531_12875"
     CDS             complement(3389..4720)
                     /gene="glnA"
                     /locus_tag="VF531_12875"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7714900.1"
                     /translation="MCIMTRDDIVQLCRDRNVRFIRLQFTDILGTVKNVSVPVSQLER
                     VLNNEVMFDGSSIEGFTRVEESDMVLKPNLDSFAIFPWQPNDGPAARIICDIYTPESV
                     PFEGDPRFILKKAIVEAEAYGYQINIGPECEFFLFKSNEDGTPSIVTHDKGGYFDLSP
                     SDLGENARREIILALEEMGFEIEASHHEVSPGQHEIDFKYADPLRTADRVTTLKFITR
                     IIAQHHNLHATFMPKPIFGINGSGMHIHLSIFKDGTNIFFDPEAKNQLSPVALYFIGG
                     VLKHIKGLTAITNPLVNSYKRLIPGYEAPVYQSWSVSNRSALIRVPAGRGNSTRIEIR
                     NPDPCCNPYLAFGLLIRAGLDGIINQIPPGEPIQKNVYTMTDVERSLGNISSLPVNLY
                     DALLEMEKDPLVRETLGDHIFERFVEAKSIEWEVYRSQIHQWEIDQYLGVF"
     gene            complement(4723..5106)
                     /locus_tag="VF531_12880"
     CDS             complement(4723..5106)
                     /locus_tag="VF531_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7714901.1"
                     /translation="MSNNDSIYPISAVMNMTKLTARQIRYYETYGLIKPARSKGNQRL
                     YTPTDVENLLLIKKLLDKGLNLDGIKAILTGNKPLEGIPNPPATPVQTGPAPNAPSPV
                     VPKLTSLYPVNNQEALNHWLQSKRK"
     gene            5285..5788
                     /locus_tag="VF531_12885"
     CDS             5285..5788
                     /locus_tag="VF531_12885"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX7714902.1"
                     /translation="MGSTSGQDHHPIINPVVNRTGAERRLARALRAQKWNFEQNQRIE
                     GYEVDFWFPEYHLVVEVDGYTHLSAVQTERDNLKDRKLIDAGYVVIRLPNQAIWNDLQ
                     GSIEMLRTLISRMKTVVSQASINDQWKTVLRQVQSELLHSGAAKAHAISSSDVEAFFL
                     SLENNGR"
     gene            complement(5762..>6010)
                     /locus_tag="VF531_12890"
     CDS             complement(5762..>6010)
                     /locus_tag="VF531_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714903.1"
                     /translation="VRRVTFNYQRLQHQFQVTLQRMNIKPAYPDIFRSAGNRKPTGLT
                     IQPQNSHQNPLKLGVIDLDDTTYKIVIRPILSPVILQA"
BASE COUNT         1600 a         1287 c         1548 g         1575 t
ORIGIN      
        1 tgaaggcgaa tgggtgaccc cgaccggagc agcccttatt aaaacttttg ccaaaagttg
       61 ctccgcccta ccactgttaa aaattagccg ggtgggttat ggcgcgggga ccgcggagcg
      121 gcctgtcccc aatgttttac ggttaatttt aggtgagaac tcccgtccga cagatcaaac
      181 cgactcccag gtggttctgg aaacgaatat cgacgatatg aatcctgaat tttacgggag
      241 attgggcgag cttctgctag agaatggggc gaaggattat ttcttaactc cggttcagat
      301 gaaaaagaac cgtcccgggg tgttggttac cgtcattacg cctcccgaac gcgtcaacca
      361 actggaaacc ctgttattca aggagactac tacccttggc atccgcaact atattgtaca
      421 ccgtaattgc ctggaacggg gcgaattgga gattcagatc tccgggatcc ccattcgagt
      481 caagactgcg atccgcgagg gtgaggtttt taagttcgct cccgaatatg aggattgttt
      541 acggaccgcg gggatcctgc aattaccggt taaggagatt tatgaccggg cgatgttcca
      601 ggccagaaaa attctccaag cagcaggagc ttcctcaagt ggcgttgaat aatgatcaag
      661 ttgttgagtt cagtcaaaat gcatttatcg agcagatctt cgaccgccta cgatcatatc
      721 tcaagtgcga tcccgattgg tctggccggg taaacaatcc gtgccaatcc tgctcgagta
      781 gttaaattca ttcggaaggg tgtgcactga tggatatcgc tgcgatggga gcatggtttg
      841 ctgcaaacgg attattgctt agctgcttta atgtaattat tgtcttactc gtcttgttat
      901 actttatcat cgctaatgcc cgtttgcgta agtatcagat gttattaaag accagctccg
      961 ggaaggattt agagcaaatg ctcctggatt tggtggaaaa aacggatctc gtctatgcta
     1021 aattgacgga ttttgagacg aaatttgaga gtaaccggat tgttgaggcc aaacacttgc
     1081 aaaaaatcgg tctcattcgt ttcaaagcct tccaaaactc gggcggtgat caaagttttg
     1141 ccctggccat gttagatgcc gccggcgatg gaatcgttat tagcagcatc tttggacggg
     1201 atgaagcccg ggtttactgt aaaccggttg agaagggcga ttcatcctat cccatgtccg
     1261 aagaggagaa ggaagccatc gtcaaggccc ttgatctgaa aaagaaatga tcttcctttg
     1321 agatagcgag agtattacag cagctaaaaa acttcaaatt ccaaaactta aaagtaaaaa
     1381 gcaggaaaag aaagcttttg tgtcgaatat taatcatcca ctcataaaca tttaaaattc
     1441 atcgatcaag gaaggcattg cttggtgttt ttcatgattg atccaaatcg tgaaagctct
     1501 tagttggggt aagacccttc cggaggtgtt ttttgtgagt aagatgatga aaacaatcaa
     1561 aaaacgtttt ggtaaaggtt ttacgttttt gattgttccc aataccggag gcgcggtcaa
     1621 gagttatgca attccctttt ccctggcttt gttgattatc gggattattg gttttaacct
     1681 atacatattt gtcggttaca caacccagat tggacagatt caagaatttc gcaccaaaat
     1741 taagacaaga gacaaaaaaa tagattctct actttcggaa cagcgggaag tccgtcccac
     1801 tttgcaaaag agttaccaga tggtcgagga tctcagcgaa ttaaagcaag aacgctcccg
     1861 aatccttagt acctggcgga atgttctgca gaaaggcgga cgatccacaa caaccagccg
     1921 gggcacgatc attcgtaaca cttcatataa tctctccacc cgtccgcggg tagagaatga
     1981 gaccaaatcc gaactggccg atctgagcta taacatgaat caggtggaag gtttcataaa
     2041 ggaagaagct caatctcagc atcaattgtg ggtggattta ttagcctatg aacggcgtct
     2101 ggaccatacc ccctcgattt ggccggtacg ttcattcatc acttccttat tcggttttcg
     2161 gcttcatccg acattgggtt attataaaga acataccgga atcgacctgc aggcccgtta
     2221 cggctcgcgc gttgccgcag cagccgatgg cagggtggcg ttttcaggat ataagagcgg
     2281 atacggttat actgtcatac tggatcacga gtatggttat cagacacttt acggtcataa
     2341 ttcgaaatta ttggtccggg tgggacaggt aatcaagaag ggacagatta tcagcctttc
     2401 aggcaatagc ggtacaagca cagggcccca tcttcattac gaggttcatg tcaataatca
     2461 tgcagttaac ccgagttcat tcctaagaaa ttagtgggga ggcaaaatca ttatgtgcgc
     2521 gaaagagcgg gacgggttta atacccggaa ggtcgacacc ttgatcggaa aggaaaccag
     2581 cgttaaaggt aatcttgatg ctcccggcgg agcggtccgg attgatggtt attatgaagg
     2641 ggaactacat atcggtggcg atctgatcgt tggtgaaacc ggcagagtga tcgggaatat
     2701 tgtggcgaaa aatgtagtga ttgccggtga agtgaaaggt tcgatagaat cccgtggccg
     2761 tctggaactg gcaccgaccg caaaagttat cggtgactcc aagatggtca ctttggtaat
     2821 tgaggacggg gcgttccttc agggtatgtg cgcgccgtta ccccggggcg atctcaaaga
     2881 acggggtaag gcattgctgg ttgacacccc tgaacctgaa aaggcttaag cgggtcccaa
     2941 gaaagctaaa acgaaaaggc ttattccttc tacggaatgt agcctttttt cattccgcaa
     3001 cgagaacccg gaaattcatt tcaccctaag cggcgaatgg ccagagtgac ggctggacag
     3061 aatagcctct tgatagaagt ttatgtttcg cgccaaaaat atgcggggag ggatttcatt
     3121 aatgcaaagg acagtcgctc tggaatcagg aattcaagtt ttaaagccgt atctgaagga
     3181 tgccggttat cgggtggtgg atctgggagg aaatcctcaa aagcaaaatc tggatgccgt
     3241 ggacgcggtg atcatctcgg ggttggatga aaatctcacc ggggattcga ccagggtcac
     3301 tcccggttgg gtgctgaatg cccgcggccg gcaaccggaa gagatccttt atgatctgga
     3361 gaaacatttt cgactgatgg attaggaatc aaaagacccc tagatattga tcgatctccc
     3421 attgatgaat ttgggaacgg tagacttccc attcaatgga tttggcttcc acgaaacgct
     3481 cgaagatgtg atcgcctaag gtctcccgta ctaaagggtc tttttccatt tcaagcaatg
     3541 cgtcgtataa atttaccggc aggctggata tatttcccag agaacgctcg acatccgtca
     3601 tagtatagac atttttctgg ataggctctc cgggaggaat ttggttgatg attccgtcta
     3661 aaccggcccg gatcagtaaa ccaaaagcca gatacggatt gcagcaagga tcgggattgc
     3721 ggatttcgat ccgcgtgcta ttgcccctgc ccgctggaac gcgaatcaac gcactgcgat
     3781 tggagacaga ccatgattga tagacggggg cctcataacc cgggatcaat cgtttgtagg
     3841 agttaaccag ggggttggtt atcgctgtaa gccctttgat atgtttcaga acaccgccga
     3901 tgaaatataa ggccaccgga cttagctggt ttttagcctc cggatcaaag aagatattgg
     3961 ttccgtcttt aaagatggat aaatggatat gcataccgga accattgatc ccaaagatcg
     4021 gtttgggcat gaacgtggca tgcaggttat gatgttgcgc aataattctg gtgatgaact
     4081 tcaatgtggt cacccggtct gcagttcgta ggggatcggc atacttgaaa tcgatctcat
     4141 gttggccggg ggagacttca tgatgggacg cttcgatctc gaaccccatc tcctctaagg
     4201 cgagaataat ttcgcgccgg gcgttttctc ccagatcgct gggggagaga tcgaagtatc
     4261 cccctttgtc atgggttacg atggaggggg taccatcctc gttggatttg aagagaaaaa
     4321 actcgcattc cggcccgata tttatctgat atccataggc ttccgcctct acgatggcct
     4381 ttttaagtat gaaacgggga tctccttcga atggaactga ttccggagta tagatgtcac
     4441 aaatgatcct ggccgcgggt ccgtcatttg gttgccaagg gaatatggcg aaagagtcca
     4501 gattgggttt taaaaccata tccgactctt ccacccgggt aaacccttca atggaagaac
     4561 catcgaacat gacctcattg ttcaagaccc tttccaactg gctgacaggc acggatacat
     4621 ttttgaccgt accgagaata tctgtgaact gcagtctgat aaaacggaca tttcgatccc
     4681 gacaaagctg gacgatatcg tcccgagtca ttatacacac cttcatttcc gtttcgattg
     4741 taaccagtgg ttcaacgctt cctggttatt gacagggtaa agagaggtca actttggaac
     4801 gactggactt ggcgcattgg gggccggtcc ggtctgtacc ggtgtcgccg gaggattcgg
     4861 tattccctca agtggtttat ttccggtcaa gattgcctta atgccgtcaa gatttaagcc
     4921 cttatccagt aatttcttga tcaataacag attctcaaca tcggttggag tgtatagacg
     4981 ttgatttccc ttggagcggg cgggtttgat caacccgtaa gtttcgtaat atcgaatctg
     5041 gcgggcagtc agcttggtca tattcatcac cgcgctgatt ggatagatag aatcattatt
     5101 actcaaaggt ttcaccttgc tttgttcatt attaccgtat gacacactag tctctcaggg
     5161 taatcgtcta aataatcgaa tcggtcattc ggtaatgttt atggattcag ttaaaagtat
     5221 acgggaatgt taggcagatg tcaacgatta tccaaaatat ggtatttgga aggaggagaa
     5281 aagaatgggg tcaaccagtg gtcaagatca tcatccgatc atcaatcccg tcgtcaaccg
     5341 taccggtgcc gagaggcgac tggcccgggc tctgcgggcc caaaaatgga attttgagca
     5401 gaatcaaaga attgaaggct atgaggtgga tttctggttt cccgaatacc atctggtggt
     5461 ggaagtggat ggttataccc atcttagtgc cgttcaaact gaaagggaca acctcaagga
     5521 caggaaatta atcgatgcgg ggtatgtggt gatccgccta cccaaccaag cgatctggaa
     5581 tgacttgcag ggttccatcg aaatgttgag gacattgatt tccaggatga aaaccgtggt
     5641 atcgcaagcg agtatcaatg accaatggaa aaccgtgctt aggcaagttc agtcggaact
     5701 tttgcattcc ggtgcggcca aagctcatgc catttcaagc agcgatgtgg aggcgttttt
     5761 tttaagcctg gagaataacg ggcgatagaa tgggacggat tacaatctta taggtggtgt
     5821 catctaaatc gattaccccg agtttcaaag gattttgatg tgaattttgc ggttgtatgg
     5881 ttaaacctgt tggtttccgg ttgcccgcgg atcggaaaat atccgggtat gccggcttaa
     5941 tattcatccg ctgtaatgtg acttggaatt ggtgttgcag gcgttgatag ttaaaggtta
     6001 cccgccgcac
//
LOCUS       DASVMB010000461        10667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_279720,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000461 DASVMB010000000
VERSION     DASVMB010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279720"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VF531_12895"
     CDS             complement(<1..599)
                     /locus_tag="VF531_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714904.1"
                     /translation="MPTLSKNNSKDPAVKDQPEDTGLAISHALLNRLVDNGQSTPSKE
                     PVQVQVKLYKDPRTPDKPKLELEIGTDRLYTVRHLSDFLEALASNQPLEINRAFTFNP
                     ERHCFAAQDQALIEMLLEAHQDEHNSWNYYFGRSEFSKGSFELNQSRFKRFLEVAATM
                     QSAFWVAPQLASPVPILICRDSLPFVLHLTEGRSHPELE"
     gene            complement(1016..1213)
                     /locus_tag="VF531_12900"
     CDS             complement(1016..1213)
                     /locus_tag="VF531_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7714905.1"
                     /translation="MQGKVKWFNPGKGFGFIETEGGADVFVHFSAIQMDGYKTLEEGQ
                     TVEFDIIQGERGPQAANVTKA"
     gene            1616..2617
                     /locus_tag="VF531_12905"
     CDS             1616..2617
                     /locus_tag="VF531_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX7714906.1"
                     /translation="MRVGLPSALLNPFYSVFWRIFFTELGVEVVESGPTTKSVLDKGV
                     RHSVPEICIPIKIYTGHVQELLDRGVDLVYIPRFVSIRRGDTFCPKFLALPDMLKCTI
                     PDLSGRMLTHHLTSRNDDIATLSNYQEIGARFTSNPNRIKAAIHAARDQWLEFRRLCY
                     SRDYNCQTANAKLAGGKDLKTAPHPVRIGVIGYVYNVYDGFISMDILQRLTNLGVRPV
                     TFEMLDENELRGKLRRDPKDLFWTFSNKLWAAANMFFEDPAITGVIHVTAFGCGPDSF
                     LGKVLALEALKYNKPFLTVRVDEHTGESHLQTRIEAFTDMILKKSPESVYSKTERIS"
     gene            2617..3723
                     /locus_tag="VF531_12910"
     CDS             2617..3723
                     /locus_tag="VF531_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714907.1"
                     /translation="MKITFPYMGSTIIYQKLLELLGYEVVMPPRPTQRTISLGVKYSP
                     EFACFPYKVIMGSYIEALEAGADTIVTSGGNGPCRAGFYSEVHLRTLRSLGYNVEMIV
                     FDNFYREPLRFIRKILRLKGKNSWNHVARVVSTTYRMAHAIDSLQKMVEYNRPYELER
                     GSFTKVFDTISRRFEVEVKTVADVRRLQEQGLRMIEAIPVRKPPQNQRIRIGVVGEIY
                     MVIESSINMGIAELLNNLGCEVSRSMYISEWVDHVLPSFLGGRSEAAIAQKGARYLEI
                     PIGGHARNNIGNMVHYREQGFDGIVHLMPFACLPELVTQSMAPVISGDLGIPILTLAI
                     DEQTGTANILTRLEAFIELVKNNKMNCAHARETA"
     gene            3739..4755
                     /locus_tag="VF531_12915"
     CDS             3739..4755
                     /locus_tag="VF531_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX7714908.1"
                     /translation="MVQAYLGVDVGSVSTNIIALSLENRVLSAQYIRTDGQPLECVKN
                     GLRRLHAEIGDLEVLGVGTTGSGRQLAGIILGADIVKNEITAHSTATVHFYPEVRTIF
                     EIGGQDSKIILIKNGMIVDFAMNTVCAAGTGSFLDHQAERLKVPIEEFGDLALTSTRE
                     VRIAGRCTVFAESDMIAKQQYGFSKADIIKGLCEALVRNYINNLGRGKILEPPYVFQG
                     GVAANKGIIAAFEKEVGCKITIPPYYNVMGAVGAAMLAREYLEKAGQTTRFAGFETSN
                     IDFIPNTFNCNGCPNLCEVVEVSADGQVVARWGDRCGKWTNAIMGTHSDNHSAVDEQV
                     KNSA"
     gene            4962..5126
                     /locus_tag="VF531_12920"
     CDS             4962..5126
                     /locus_tag="VF531_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714909.1"
                     /translation="MKDINEESKDSQNDQVKSEPVPETRNSGIDGKLAQNLGDGRWQY
                     HGKTYKEQAI"
     regulatory      5489..5662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5754..7109
                     /locus_tag="VF531_12925"
     CDS             5754..7109
                     /locus_tag="VF531_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7714910.1"
                     /translation="MENQGKPELKKGLGLAAATALVVGNMIGSGIFMMPNTLASSSGP
                     GASLIAWGFTGIGSILLALTFANLGGRYPQTGGPYAFAKIAFGEFAGFINAWIYWIGG
                     CIGNAALLTAFAGYLAFFIPVVRESGMIAFVITSALLWLFTYINIKGVREAGVVNLIT
                     TVLKILPLIFFIIIGLQHFDIRIAGPMFPKGRGLATIPAAAAATLWAFIGFETATVTA
                     GEIRNPERNVRLSTIWGIALTTLVYLGISLVAMGTMSQQALANSSSPLADIINQFASG
                     WGASFIALGGFVSTLGATLGWVLSTARMSYAAAEDGLLPKGMAAVHPKYCTPHISLII
                     SGVIANITLLLFYVRSLTAAYNFMILLATLSFLPPYVFAAAAEIMLLAKGSPRFSLGN
                     FIKSSIFSLLGFAYGIWTIYGSGAETVMYGFILMLLGIPIYVYMKLKSQAHSSRAETA
                     V"
     gene            complement(7249..8199)
                     /locus_tag="VF531_12930"
     CDS             complement(7249..8199)
                     /locus_tag="VF531_12930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7714911.1"
                     /translation="MKIWISWIIGLILSFTTNVVFAATVVPVKATAAVLINAETGEVL
                     FSKNPNSVMAPASTTKIMTAIIAIEKGNLGHKVTVSRRAASMPPTSMRLRRGDVISMK
                     DLLYGLLLSSGNDAAVAIAEDIAGSEAGFAKIMTTKARSLGMMNTVFKNASGLPASGH
                     HTTAHDLAILAGYALKNPTFAAIVQTKVASVPRSRSSSRSLVNHNKLLWQYPYTTGIK
                     TGYTRAAGPCLVASATQNGVSLISVVLKSGNMYQDTIQLFDFGFSNASEPEPTPTPEP
                     TPTPTPEASIPADIPRYFYYEATHTLPFQSSTEPTPTPVQ"
     gene            8606..9517
                     /locus_tag="VF531_12935"
     CDS             8606..9517
                     /locus_tag="VF531_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714912.1"
                     /translation="MANRAVLFHDIQIYLFQSHPWWGLTVLTLIVALIFIGYYYQYQI
                     KPRKAQAKPDVTSKFRIMFLTTSVIVFALASSYRVLFTPVRTVQGVWAILSPPLLFVL
                     VLFLKILLLCWIIRLLTMLFKPELLSEFGAKFFGFEIHQKYNPQQIQDGLRKIEKQLD
                     ILARLNGSALTIISTPFERYFLQSENRADRIRQLVKDVLIDVYETLPEVKIYVIPFTD
                     AGIGQLPEVLAAKVRLKSIEKVNSTQVENDRIGMSIHHGTQGSETVIIIDTTKQNYDL
                     SLAEITAAAHLFVSIATILDWAVKSRT"
     gene            9912..10088
                     /locus_tag="VF531_12940"
     CDS             9912..10088
                     /locus_tag="VF531_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714913.1"
                     /translation="MPLKKQPPGIPIVRGRQKTPRIFEELRGLLRLNDEPEEDREGNF
                     DRIDLEKYFVRNKS"
     gene            complement(10475..10652)
                     /gene="ssrS"
                     /locus_tag="VF531_12945"
     ncRNA           complement(10475..10652)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF531_12945"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         2658 a         2482 c         2730 g         2797 t
ORIGIN      
        1 aactccagct cgggatggga gcgcccctcc gttaaatgca ggacgaatgg gagtgaatcg
       61 cggcagatca ggatgggaac cggtgaagcc agttgaggcg cgacccagaa agcggattgc
      121 atcgtagcgg cgacctccaa aaatcgttta aaccgtgatt gattcaattc gaaagagccc
      181 ttcgaaaact cggagcggcc gaagtaatag ttccatgagt tatgttcatc ttggtgggcc
      241 tcaagcagca tttcgatgag ggcttgatct tgcgccgcaa aacaatgccg ttccgggttg
      301 aaggtaaaag cccggttgat ttcgagcggt tgattggagg ccagggcctc gagaaaatca
      361 ctcaaatgtc tgacagtata aagccgatcg gtgccaatct ccagctcgag ctttggttta
      421 tccggtgtcc gcggatcttt atacagtttt acctggacct ggactggctc ctttgaggga
      481 gtcgactgcc cattatcgac caacctgtta agtaaggcgt gggagatggc gagtccggta
      541 tcttccggtt ggtcttttac agcaggatct ttagaattat tttttgatag cgtcggcatg
      601 ggatctcctg aatagattgg actggttcgc ttgggatcgg gaacgttttt caaaagaaag
      661 tttcgaatac cttgagatta tcaaaaaatc cgtgccaagg caagtaagga ctttgctttt
      721 gtctcctgcg gttggctttt ctttaattat tccctggaag agctggtatc tctcaaaaat
      781 ttaaccaacc tgcgacttac ggccgaaccc tattattact acggaacata ttacacctgg
      841 gggtgcgctt gatttaccgg ccgcagcaac cggcggaacg gattgaactg caattccgaa
      901 cggtttcaaa cggataatgg gtcacttata ttcctgatca aaaggttcaa ccggagccat
      961 ctagggcaaa agaaaaaacc ccggttttgc cggggttttc tggtttgcat cgaaattatg
     1021 ctttcgtgac gtttgcggct tggggtccgc gttcgccttg aatgatatcg aattcaaccg
     1081 tttggccttc ctctagggtt ttgtatccat ccatttgaat cgctgagaag tggacgaaca
     1141 cgtcggcccc gccttcggtt tcgataaatc cgaaaccttt tcccgggtta aaccatttta
     1201 ccttgccctg catctgcatt cctcctaaaa aaccttgacc ttatttttat cacaaagtca
     1261 tgtaaatatt actatagcat aggatggcag tcctgtcaaa tcaaaacaag tccattgaca
     1321 gttttttcac ggattggtct tacgttccgg gacgggtttt ccaaagtggc agggagtatg
     1381 aaaaacaggc gactccgtcc gtaaaggggg agtataaaat tttacacggc tttaaaggga
     1441 atatcaaagg catcattccg cggaattttc ccgaaacctt ggatggttaa tggaagccga
     1501 agttgcaaaa tacgttacaa tgcgttacaa tcgaaatcaa agcgcatttt ttcatattga
     1561 atttcaggct tttggggttt tttcgtaatc ttcgggtatc atgggaggag tattcgtgcg
     1621 agtcgggctg ccgtctgcct tgttaaatcc attttatagt gttttttggc ggatcttctt
     1681 tacagagctg ggtgtggaag ttgtggaatc gggtcccacc actaaaagcg ttttggataa
     1741 gggagtccgg cattcggttc ccgagatctg cattccaatt aagatctaca ccggacatgt
     1801 ccaggagttg ctggaccgtg gggtcgatct ggtttatatc cctcggtttg tctcgatccg
     1861 gcggggggac accttttgtc ccaagttttt ggcccttccc gacatgttga agtgtacgat
     1921 tcccgacctc tccggccgga tgctcactca ccatctaaca tcccgtaacg atgatatcgc
     1981 gaccctttcg aattatcaag aaatcggagc ccggtttact tccaacccca atcgcataaa
     2041 agcagcgatc catgcagccc gggatcagtg gttggagttt cggaggttgt gttattcccg
     2101 ggattataac tgccaaaccg ccaatgccaa attggctggc ggcaaggatt taaagacagc
     2161 gccgcatccg gtccggatcg gtgtaatcgg ctatgtatac aatgtctacg atggttttat
     2221 cagtatggat atcttacaac gtctcacaaa tcttggagtc cggccggtaa cctttgagat
     2281 gttggatgag aatgagctcc gcggcaaact gcggcgggac cccaaggatc ttttctggac
     2341 tttctcgaat aaactctggg ccgcggcgaa tatgtttttt gaggatccgg ccattaccgg
     2401 agtgattcat gtaacagctt tcggctgcgg accggattcg ttcctgggta aggttttggc
     2461 actggaggcc ctaaaatata acaagccgtt tttgacggtc cgggtggacg aacataccgg
     2521 agagagtcac ttacagaccc ggattgaggc ctttacagat atgatcctaa agaagagtcc
     2581 cgaaagtgta tattcgaaaa cggagcgtat ttcttgatga agatcacctt tccatatatg
     2641 ggttcaacca ttatttatca aaaactattg gagcttttgg gttacgaggt ggtgatgccg
     2701 ccccggccaa cccaacgcac catcagcctt ggagttaaat acagccccga gttcgcctgt
     2761 tttccctaca aggtgatcat gggttcctat atcgaggctc tggaagccgg ggcagacacc
     2821 attgtcactt ccggcgggaa tgggccttgc cgggcaggat tttacagtga agtccacctt
     2881 cggacccttc gtagtctggg gtataacgtc gagatgatcg tcttcgacaa tttttaccgg
     2941 gaaccgctcc ggttcatccg gaaaatcttg aggctaaaag ggaaaaacag ttggaaccat
     3001 gtggcccggg tagtttcaac aacctaccgg atggcccatg ccatagactc cctccaaaaa
     3061 atggtggaat acaatcgtcc ctatgagttg gaacgaggtt cctttaccaa ggtgttcgat
     3121 accattagcc ggcgttttga ggttgaggtg aaaaccgtgg ccgatgtccg ccgtttgcag
     3181 gagcaaggtt tgagaatgat cgaggcgata ccggtccgga aacccccgca aaatcagaga
     3241 atccggatcg gggtggtggg cgagatctat atggtgattg aatcatccat caatatgggc
     3301 atcgccgagc ttctgaataa tttgggctgt gaggtcagcc gatcgatgta catctcggaa
     3361 tgggttgatc atgtgctccc ttcgtttctg ggcggcaggt ccgaggccgc catcgcccaa
     3421 aagggcgccc gatatctgga gatacccatc ggcggccacg cccggaataa catcggtaat
     3481 atggtccact accgggagca gggatttgac gggatcgtac atttgatgcc ctttgcctgt
     3541 ctgccggaat tggtcaccca gagcatggct ccggtcatct ccggagatct gggtatcccc
     3601 atcctgaccc tggccatcga tgagcaaacc ggtaccgcca atattctgac ccggttggaa
     3661 gcctttattg aactggtcaa gaataacaaa atgaattgcg cgcatgcacg cgagaccgcc
     3721 tgaggaggtt attattgtat ggtccaagct tatcttggag tggacgttgg ctcagtgagc
     3781 acaaacatta tcgccttgag tctggaaaac agggttttat cagcccagta tatccggaca
     3841 gacggccaac cgcttgaatg cgttaaaaat ggacttaggc ggcttcacgc cgagatcggc
     3901 gatctggagg tactgggagt cggtacaacg ggaagcggtc gccaactcgc ggggatcatt
     3961 cttggggcgg atattgtcaa gaacgagatc acggctcatt ccacggcaac agtccatttc
     4021 taccccgagg ttcgaacgat ctttgagatc ggcggacagg attcgaagat cattctgatc
     4081 aaaaacggaa tgatcgttga ttttgcaatg aacacggttt gtgcggctgg aaccggatca
     4141 ttcctggacc atcaggctga acggctcaaa gttcccatcg aggaattcgg ggatctggcc
     4201 ttgacttcca cgcgtgaggt tcgaattgcc ggaaggtgta cggtctttgc cgagtcggac
     4261 atgattgcca agcaacaata tggtttctcg aaggccgaca tcatcaaggg gctttgtgag
     4321 gctttggtgc gaaattacat caacaatctg ggacgcggaa aaatattgga accgccgtat
     4381 gtatttcaag gcggggtggc ggccaataaa ggaatcattg cggcttttga gaaggaagtc
     4441 ggctgcaaga tcaccattcc tccgtattat aacgtaatgg gcgcagtagg cgcggccatg
     4501 cttgcccggg agtatctgga aaaggccggc caaaccaccc ggtttgccgg atttgaaacc
     4561 tcgaatatcg attttatccc caataccttt aactgcaacg gctgccccaa tctctgcgag
     4621 gtggtcgagg tctccgccga cggccaggtc gttgccaggt ggggtgatcg ttgcggcaag
     4681 tggaccaacg ccatcatggg cacgcacagt gacaaccaca gtgctgtcga cgaacaagtt
     4741 aaaaactcgg cgtaaggtag ttttttcttc cgggataccg caaacccata cagtatgaca
     4801 atttttggca ggtgaaatgt gctttcgcgg gaaataatcg atacggaacc gggaaaaagt
     4861 tccgtcttta ttgggatgga aatattgatt ctcacaataa atgatataat ataagtaacg
     4921 aaaccaccat ggggattaaa cgaataagag gtgaaacaac gatgaaggat attaatgaag
     4981 agtcaaaaga ctcccagaat gatcaggtaa aatcggaacc ggttccggaa acgaggaact
     5041 cggggatcga tggtaaattg gctcaaaacc tgggagacgg acgctggcag taccatggca
     5101 agacctataa agaacaagcg atttaaagct ccgctcttgc tgttacgtcg ggatgtttta
     5161 ttaaggaggt ctgcgtaata acaggccgcc tttctcccgg ttttccccct tgaaatgcct
     5221 gactgtcggt tgtacatccc tccgctatcc acttgttccg cattacgcga atcaatccga
     5281 tttcagagtg tactgaaata gttatacaac catttgaata attattcaaa catgtatatt
     5341 ttcgtatact caaggatgaa ctggcttatc cacgctttct ccaccccaat tgaacaattc
     5401 cttcctggct gtgtttgaca gggcttcatc atccttgcta cacttaaatc acatgccaat
     5461 gacatgaaag aataaacaat attgctaaaa gctgagtaga ggcgcggaaa ttatcagtaa
     5521 cggggatttt tgagcaaatg ggtccccgcg aaaggaatct ccgccgaagt ccgggacggt
     5581 ggctcaaccg tttcgggctg ggcccgtacc gaaaaggcac gggactgtca tcgatcgcaa
     5641 tcgatggaga gctattccgc ggtatcgtag tctcgcgcgt aaacgcgcgt caggcgccgg
     5701 ggaatacccg gcgcctttct gtgcttctgg ggaagtaaaa cgaggaggtt acaatggaaa
     5761 accaagggaa acccgaatta aagaaagggt tgggactggc ggcagccacc gcgctggtgg
     5821 tggggaacat gatcggctcc ggtattttca tgatgcccaa taccctggcg agcagttccg
     5881 gtccgggcgc ttcattaatc gcctggggat tcacaggcat cggatcgatt ttgctcgcgt
     5941 tgaccttcgc caatttggga gggcgttacc ctcaaaccgg aggtccgtat gctttcgcca
     6001 aaatcgcctt tggtgaattc gccggattca tcaacgcctg gatttattgg attggcggtt
     6061 gtatcggaaa tgccgcatta ttgacggcct tcgccggtta cctggcattc tttatcccgg
     6121 tggtccggga gagcggaatg atcgctttcg ttatcacatc ggcattactc tggcttttta
     6181 cttatattaa cattaaaggg gtacgcgaag ccggggtggt caatctgatc accaccgtat
     6241 taaagattct gcctctcata ttcttcatca tcatcgggct gcagcatttc gatatccgga
     6301 tcgccggtcc catgttcccg aaagggagag ggttagccac gataccggct gcagcggctg
     6361 ccacattgtg ggcatttatc ggttttgaaa ccgccacggt aactgcaggg gagattcgca
     6421 atcccgagcg gaacgttcgt ctcagcacca tctggggtat tgccctgact accctggttt
     6481 atctggggat tagcctggtt gctatgggaa ccatgagtca gcaggcgttg gccaattcca
     6541 gctctccgct ggcggatatc attaaccagt ttgcgagcgg ttggggcgca tccttcattg
     6601 cgctcggcgg ttttgtctcg acgctcggag ccactcttgg ctgggtgctt tccacggccc
     6661 ggatgagcta tgccgcggcg gaggatgggt tgctccccaa gggtatggcg gcggtgcatc
     6721 ccaaatattg cactccccat atctccctta ttatcagcgg cgtcatcgcc aatatcacct
     6781 tgctcctgtt ttacgtgcgt tccttgacgg cggcctataa cttcatgatc ctgcttgcaa
     6841 ccctctcgtt cctgccgccc tatgtatttg cggccgcagc cgagattatg ctgcttgcca
     6901 aaggaagtcc aaggttttca ctgggaaact tcatcaaatc atcaatcttt tcactacttg
     6961 gtttcgcgta cgggatctgg accatttacg gctcaggcgc ggaaactgtc atgtacggat
     7021 tcattctgat gctgctgggt attccaatct acgtttatat gaagctgaaa agccaggctc
     7081 attcctcccg ggcggagaca gcggtgtgat agttcgttcc acataggata gtttctggtc
     7141 caattagaaa gcggtatcgg atcttcatat ccggtaccgc tttcgttatt tttcatagct
     7201 cattttcatt gtttttaaga attcgaattc taacccccgg attagggctt actgaaccgg
     7261 cgtgggagta ggttcggtag agctttggaa cggcaaagta tgggtggctt cataataaaa
     7321 gtagcgcgga atatccgccg gaatggaggc ttcgggggtg ggggtcggtg tcggttccgg
     7381 ggtaggggtc ggttccggtt cagatgcgtt cgaaaagccg aagtcgaata gttggatggt
     7441 gtcttgatac atgttcccgg atttcaggac taccgaaata agggagacac cgttttgcgt
     7501 ggctgaagcc accagacagg gcccggcggc gcgggtgtag ccggttttaa tcccagtggt
     7561 atacggatat tgccacaata gtttgttatg attcaccagt gaccgggagc tggaacggga
     7621 acggggtacg gatgccacct tcgtctggac gatcgccgca aaagtcggat ttttcaatgc
     7681 ataaccggct aatatcgcca gatcatgtgc ggtggtatgg tgaccggatg ctggcagacc
     7741 gctggcattt ttgaagaccg tattcatcat tcccagggaa cgggccttag tcgtcattat
     7801 tttggcgaag cctgcctcgg aaccggcaat gtcctcggcg atagctactg cagcatcgtt
     7861 tcccgaggat aaaagcaggc cgtagagtag atccttcatg gaaatcacat cgccacggcg
     7921 cagacgcatg ctggtggggg gcattgatgc agccctacgg cttactgtaa ctttatgacc
     7981 gagattgcct ttttcgatgg cgatgatggc tgtcataatc ttggtggtgc tggcgggagc
     8041 catgacacta ttgggatttt tggagaaaag cacctcgccg gtttcggcat tgatcagcac
     8101 cgctgccgtg gccttgactg gaacgaccgt tgcggcgaat acgacattcg tggtgaaact
     8161 cagtatcaat ccaatgatcc aactgatcca tatcttcatt gatcgtgcac cccgatgttt
     8221 gtaatttgtt aagatttaat ttccaggaag taaaaacagt ctccctatta ttttcgtatg
     8281 atggccagaa tttctttaca gttttttggt aataagctgg gtgttttcat ctatttagcc
     8341 tgcaccattc agttgaaaag cggcttcgta tgtaaacttc ccggtaaaca aagaaaaacc
     8401 tgccgaatca tttggctttg actgaaatat aacataatca aagggttttt cgggagataa
     8461 cagactgacg taccggttga catctggggt aaaataacat tttttcaaaa tgtgaggaaa
     8521 acgaatggct aaatgactga cagaatagaa tagatatggt ataataaggt aattaagaaa
     8581 atttaaaatt ttgtggtgag gattcatggc aaatcgagcg gttctcttcc atgatatcca
     8641 aatatacctg tttcagtcgc atccgtggtg ggggttaacg gtgcttaccc ttatcgtggc
     8701 gttgattttt attggctatt attatcaata ccaaatcaaa ccccggaaag cccaggctaa
     8761 acccgatgta acatctaaat ttcgaatcat gtttctgact acatccgtaa tcgtctttgc
     8821 cttggccagc tcataccggg tgttatttac tccggtccga accgttcagg gggtttgggc
     8881 tatcttaagt ccgccgttat tatttgtatt agtattgttc ctaaagattc tactgctctg
     8941 ttggattatt cgcctactca caatgctttt caagccggaa ttgctctccg aattcggcgc
     9001 aaaattcttc ggttttgaga tccatcagaa atacaatcct caacagattc aggacggttt
     9061 acgcaagatc gaaaagcaac tggatatcct ggcgagattg aacggttcgg ccttgacgat
     9121 catttcgacc cctttcgaga ggtatttcct tcagtcggag aaccgcgccg atcgaatccg
     9181 ccaacttgta aaagatgttt tgatcgatgt ttatgaaacc cttccggagg ttaagatata
     9241 tgtaataccc tttaccgatg ctggaatcgg ccaacttccg gaagttttgg cagccaaggt
     9301 gcgattaaaa tccattgaga aggtgaacag cactcaagtg gagaatgatc gtatcgggat
     9361 gagcattcac catggcactc aagggtcaga gacagtcatt attattgata caacgaaaca
     9421 gaattatgat ctatccctgg ccgagattac cgctgccgcc catctctttg tatccattgc
     9481 caccattctg gattgggctg ttaaaagcag aacgtgagtt gaacctcgat tttataatcg
     9541 agtataaata ccagggatgg tccttatcaa actgggggga ttatggttgg ccttacaaaa
     9601 gcgtagagta gaaattcgtg gttcgagtac gccgatgtac atcaaggatt taatcgcctc
     9661 gacccgggag agtccagaaa atcctcaacc tgccagagcc gaaacctctg ctgtcttcca
     9721 gaaagagcgg aatccacgca atatcgggaa agtttccgta accaaaataa tcaaaggttc
     9781 ggattcagag gaaggattgg agattcaggc tgcctcccga ataaagaaac cgtgtttggc
     9841 aggtgggaca agtaaaaaat aggtgataga atcggtccgt gggtaatact atacacctgg
     9901 aggtgttggt attgccttta aagaaacagc cgccgggtat tcctatcgtc cggggccgtc
     9961 agaaaacacc ccggatcttt gaggaactcc gcgggttgtt gcgattaaac gacgaaccgg
    10021 aagaggatcg tgaaggaaac tttgatcgga tcgatctaga gaaatacttt gtacggaata
    10081 agtcttaacc ggaaattcac ctcgcaagtt cccggtctcc gctttttccg ctacacactt
    10141 gagtcgttcc gccattgacg ttcaataatt tcccttaaat tacaggattt tctcaaaacc
    10201 tgggagaaga tagatcagct tcgcaccctt gtatcctgca tctgccttaa ctttggcttc
    10261 aattacttat aacagagcaa aaaaaaaccg gcggcctgcc ggttttttcg taggaaccgc
    10321 ggcatacaag actcctatcg gagccgatgc ctctgaaatc ccacagtcgc aattcacggt
    10381 tgatcgagcc gtgccggcag atacgacgat tggcttgcgc cgccgccatt actatcatag
    10441 ccatctgcag cccggtttat accccaaaag tatgtttata tccaagacac cgttgtttca
    10501 gttttgacac ctatctcccg ataggtgggt gatcgcacaa ttacctcaac cttcccaata
    10561 tctttcaagg cttgatttta gtaattaaat ataaatctcc cgtactcaca ctgtaggttc
    10621 aaaaatgaaa cggacgaata tcccagaaaa cttttaaaaa ggttata
//
LOCUS       DASVMB010000462        11197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_571282,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000462 DASVMB010000000
VERSION     DASVMB010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571282"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VF531_12950"
     CDS             complement(<1..573)
                     /locus_tag="VF531_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7714914.1"
                     /translation="MGLYRAHVLVCAGAGCISSGCKEVERNLLAELESHHLSEEVKVV
                     ETGCIGSCDLGPAIVIYPEGVFYQKVKPEDVQLIVEEHLLKGRPVQKLLYKQANGEPV
                     VKYEEINFFNHQLRIALRNVGLINPEVVEEYIARDGYAALGKVLTEYKPADVVDIVKR
                     SGLRGRGGAGFPTGVKWDITMKVPDDTKYVV"
     gene            complement(832..1443)
                     /locus_tag="VF531_12955"
     CDS             complement(832..1443)
                     /locus_tag="VF531_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7714915.1"
                     /translation="MRELSLHILDLVQNSLAAAATKVTIMVRELTGQNRLEIEIRDNG
                     RGIPRSSLGKVHDPFYTTRSSRQVGLGLSLFEAAARQCSGTMTVESQPGQGTTVLAVF
                     QYDHLDRAPLGNMADTILALIATNPGVDFCYIHQYDTEQYQFDTREIKEYLGCADLAA
                     PAVIAALREYLTDRYQELRVPVSQALNNLSESLTNESYVLTKG"
     gene            complement(1640..2170)
                     /gene="nuoE"
                     /locus_tag="VF531_12960"
     CDS             complement(1640..2170)
                     /gene="nuoE"
                     /locus_tag="VF531_12960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7714916.1"
                     /translation="MAEGCLNCNCCSDSDQRYQALDEIINKYRNKPGALIPVLHQAQQ
                     LFGYLPEEVQIKVAEGLDVPLSEVYGVITFYSFFSTKPRGKYTIGVCLGTACYVKGAA
                     QVLQAVKDELGIEMDDTTDDGLFTLTVTRCLGACGLAPVMMIGDDVYGRLTPERIPEI
                     LNSYRKESQQTAAEKK"
     gene            complement(2475..3545)
                     /locus_tag="VF531_12965"
     CDS             complement(2475..3545)
                     /locus_tag="VF531_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX7714917.1"
                     /translation="MEEVLRDIPAGKAGTQPRFPATRFQGSKFKLADWIWEGIKDLEF
                     DTALDAFGGTGSIGYLLKLHHKQVTYNDLLKFNWHIGRALIENDRVVLSDDELDALLQ
                     PYPGRFYPTFIQDTFHDIYFTDAENRWIDMVVTNIGCLTDPFRKALAFFALFQTCLAK
                     RPFNLFHRKNLYLRFSDVPRNFGNKATWDAPCELLFRKFASAASNAVFCNGLANTALN
                     QDVFQIEGSFDLVYIDPPYLSGKGVGVNYYQFYHFLEGLAQYDVWGSLIDYSSKHRRL
                     KGGDSAWIRKDSIYEAFDKLFARFQESILVVSYRADGIPKVGELIGLLRKYKAEVKIM
                     ERREYQYALSGRRSGEVLFIGR"
     gene            complement(3576..4316)
                     /locus_tag="VF531_12970"
     CDS             complement(3576..4316)
                     /locus_tag="VF531_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX7714918.1"
                     /translation="MPASGYCLADLHIHSALSPCADDRMTPAAVTAQARRVGLDIMAL
                     TDHNSVRNCLAFLNWDSPDLWIIPGMELQTREGVHLICLFPGMAAAWEWQDFVDRHLP
                     DLPNRSERFGHQYLFNAAGEVTGEEHRLLLNSTTLAWDEVLQRVPEYGGLVYPAHIDR
                     PSFSVLANLNRIPEERSLRIVEISAQADPARLTRDYPHLAGARLIRSSDAHRLDEVGR
                     VCTKLPVAIREWDALQSALTSPIPETGG"
     gene            complement(4318..4650)
                     /locus_tag="VF531_12975"
     CDS             complement(4318..4650)
                     /locus_tag="VF531_12975"
                     /inference="COORDINATES: protein motif:HMM:NF018753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HEX7714919.1"
                     /translation="MTLQEVANVLKAAVHSGRDQLDKPVLSGYASDLLSEVMAHAQAG
                     QLWFTVQTHLNVPAVAVLLDLGGIVITGGNRPEPAVITKGNENGMVIMTTPYTTFEAI
                     ALLCKAGF"
     gene            complement(4647..5999)
                     /locus_tag="VF531_12980"
     CDS             complement(4647..5999)
                     /locus_tag="VF531_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX7714920.1"
                     /translation="METWFHAVSLINERCKGCTNCIKRCPTEAIRVRNGKASIDGERC
                     IDCGECIRICENHAKQAEGDSLAATTGYRYRVAIPAPALMVQFQKGVRPEQILEALLG
                     LGFDRVAEAGWGADLTTVALKRYLKERYHAEPRPLISSSCPAVVRLIQIRFPGLIQQL
                     APIEPPLISAAKLARMEIMKQTGARSGEIGVFFISPCPAKITMIKNHYGIEPGVINGA
                     LSIRAIYGDLQKLIPKSDSITISSRLSKIGAGWAGAGGESRAVGIGNLLAVDGIHQVV
                     DLLEEIEMGRLNDLDYVEVQACNNGCVGGALTVINSFTARMRINQLLSDGWLPPPDPA
                     LAAQADTYYNTRFFDWDYQVIPKPFQRLDQDLSSAMEKVELIEATVRSLPGLDCGSCG
                     SPNCRALAEDIVLDKAQIFDCVFKLREELSRHAQELLLLAERKPPAMGTSTQESGEKQ
                     "
     gene            complement(6022..6492)
                     /locus_tag="VF531_12985"
     CDS             complement(6022..6492)
                     /locus_tag="VF531_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX7714921.1"
                     /translation="MSWIFPPGGIFRPMERIYPIAGGDYEASGEAAGKIKKLLIQLGL
                     AMELVRRIAIAAYEAEMNIVIHAYEGELTLRIGRDLTDARVRMRLVANDRGPGIPDID
                     LAMKEGYSTAPEFARRLGFGSGMGLPNMKRCSDQLTIISTVGKGTTVTMDFTLD"
     gene            complement(6763..7275)
                     /locus_tag="VF531_12990"
     CDS             complement(6763..7275)
                     /locus_tag="VF531_12990"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7714922.1"
                     /translation="MENKSSKGGKTITNLLSKTVGLIFFSFLAISLGVVFGLLFEGWV
                     KEQPGTPVGSENSTLQTVDLSNEKTDTVTQDTVNSDLSQPVANDTLDNVAVQPSVSVK
                     YKVRVGPYANRTQALAASTQLQSMGYPVFVGTAPPFTVQVGAFSAQNNADRLKNELVG
                     KGYKVSIDQN"
     gene            complement(7797..9464)
                     /locus_tag="VF531_12995"
     CDS             complement(7797..9464)
                     /locus_tag="VF531_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7714923.1"
                     /translation="MWEQYVTGTRFESYEDFAANFRVKVPDHFNFGYDVVDAWAEHEP
                     QKIALVWCDDKGASANYTFAEMKRYSDKAANFFRSLGIGKGDRVMLILKRRYEYWFSI
                     LALHKLGAIAIPATHLLKKKDIVYRNQAASVKVIVSVAEPDIMAEIDAAQAESPTLRY
                     KLAVGGERDGWISFDRGLKEAAEEFAKPAAAELPQNDDIMLLYFTSGTTGYPKMVQHN
                     FVYPLGHITTALFWQSIRENGLHLTVAETGWAKAAWGKLYGQWLAGAGIFVYDFDRFI
                     PHDLLEKVAHYKVKTFCAPPTIYRYLIKEDLTKFDLSNLEYCVVAGEPLNPEVYNQFL
                     KQTGLKLREGYGQTELTVVLATFPWMEPKPGSMGKPAAGYEIDIIDIYDENGRSCEVG
                     EIGEIVIRTDRQKPVGMFDGYYSDPERTRSVWHDGLYHTGDTAWRDEDGYFWFVGRTD
                     DVIKSSGYRIGPFEVESALLEHPAVLECAVTAVPDPLRGQVVKATIVLTKQYQASEAL
                     KQELQEHVKRVTAPYKYPRIIEFVNELPKTISGKIRRVEIREQDQIE"
     gene            complement(9490..10044)
                     /locus_tag="VF531_13000"
     CDS             complement(9490..10044)
                     /locus_tag="VF531_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7714924.1"
                     /translation="MQEELKQIADRIRGLREIKAVEPDTIARELGITSAEYETYESGE
                     TDIPVGILYKLAQYYHVELATFLTGEEPRLHLYSLTRNGKGVSVERRRDYKYQSLAAN
                     FVHKKAEPFLVTVEPGPETAPIHYNSHPGQEFDYCLEGTLQVMLNGHAVVLNEGDSLF
                     YDSGVAHGMKALNGKNARFLAVIL"
     gene            complement(10413..10916)
                     /locus_tag="VF531_13005"
     CDS             complement(10413..10916)
                     /locus_tag="VF531_13005"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012117807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX7714925.1"
                     /translation="MMAIIAAMDLNRLIGNERSLPWRLPDDQAYFKRLTIGHPVVMGR
                     ITFESIGRPLPDRENLVLTRGHEFQAQGCRIMRSIEEVLKYAEARPDTTVFIIGGTQV
                     YTAFLPYVRRLYITLIRDVFTGDRYFPEIDPRDWRLSSETEGAVDNLNPMAHSFLIYD
                     RIPTTTP"
BASE COUNT         2844 a         3057 c         2566 g         2730 t
ORIGIN      
        1 aaccacatac ttggtatcgt ccggaacttt catggtgata tcccacttga cgccggtagg
       61 aaatccagcg ccgccgcgcc cccgcagtcc ggaacgtttg acaatatcca ccacatccgc
      121 gggtttatac tcggtcaaca ccttgcccaa agcggcatag ccgtcacggg cgatatactc
      181 ctccactact tcagggttga tcaaacctac gttacgtaag gcgattcgca attggtgatt
      241 gaagaaattg atctcctcgt atttcacaac cggttcgcca ttggcttgtt tatacagaag
      301 tttctgcacc ggcctgcctt tgagcagatg ttcctcaaca atcagctgaa catcctccgg
      361 tttcaccttt tgataaaaga ctccttcagg atagatgaca atggccggac ccagatcgca
      421 acttccaatg caaccggtct ccaccacttt cacttcttcg gaaagatgat gactttccag
      481 ctctgccagt aagttccgtt caacttcctt acacccggat gagatacaac ctgctcccgc
      541 gcaaaccagg acgtgcgccc gatataatcc catgtcttga tcctccccag ttctggatga
      601 aagcttcatt cctgataata aactgataaa tctcccgatg gtcctgaccg gccatcatgc
      661 aaccgaatcc gtaactgact gatacgcttg tccagataaa aattaataca aagttatata
      721 ctaaaatgct ctgttttcag aaacggttaa tcccaggtat ttaaggcaag tgcagctcct
      781 tgaggggaag gggtggttga cccctagata aagctaaaag aaaatgtcgg attaaccctt
      841 agtaagaaca tagctctcgt tagtaaggct ttccgagagg ttattcaaag cctgagaaac
      901 cggcactcgt aactcttgat atcgatcggt caaatactcg cgaagcgcgg cgatgaccgc
      961 cggggccgcg agatcggcac aacccagata ctccttgatt tcccgggtat caaattgata
     1021 ctgctccgta tcgtactgat gaatataaca gaaatcgacc ccgggattgg tggcgatgag
     1081 cgccagaatc gtatccgcca tattgcccag gggagcccga tcaagatgat cgtattgaaa
     1141 gacggcgagc accgtggtac cttggcccgg ttgagactca accgtcattg tcccggaaca
     1201 ttgcctggca gccgcttcaa aaagcgaaag acccaaaccg acttgacgcg aggagcgggt
     1261 tgtataaaac ggatcatgaa ccttgccaag ggatgagcgt gggattcccc gaccattgtc
     1321 gcggatctcg atctccagac ggttttgccc ggtcaactcc cgcaccatga tggtcacctt
     1381 ggtagcggcg gccgctagag aattctgaac cagatcgaga atatgcaagg agagttcacg
     1441 catgtctttt cccgttcccc tatcccgttt tggaagtttc tttgaaccgc ccattcaaaa
     1501 cttccggata cgggtttttg attctccgta gattgttcac gatttcacaa accggaacaa
     1561 tcaataatcc ggtttcaacg gaaagccgga gtcgtattgt ggtaaaacat tgtgattgtt
     1621 ctttcaatca caaagaagtt tatttttttt cagccgccgt ttgctgagat tcttttcgat
     1681 aactattcaa aatctcgggg atgcgttccg gggttaaacg gccgtataca tcgtccccga
     1741 tcatcattac cggagccaaa ccgcatgcgc ccaagcaacg ggtcacggtc aaagtgaata
     1801 aaccgtcatc ggtggtatcg tccatctcga tccccaactc atccttgacg gcttgcagaa
     1861 cctgagccgc tcccttaacg taacatgcgg ttcccaggca aacgccgatg gtgtatttcc
     1921 cccgcggttt ggtggagaaa aacgaataaa aggtgatgac accgtatacc tcgctgagtg
     1981 gaacatcaag cccttcggcc accttgatct gcacttcttc gggaaggtaa ccgaagagtt
     2041 gctgagcctg gtgcagaacc ggaattaatg cgccgggttt gttgcggtat ttgttgatga
     2101 tctcatccaa agcctggtac cgttggtccg agtccgaaca acaattgcag tttagacaac
     2161 cctctgccat cgttaagtcc tcctgccctc atttgatgaa tcagtaagtt ataaaaataa
     2221 caaagaatta tagaaaaaaa ctttcgttca aaccgggacc attgtgagcc gaagcataat
     2281 ccgtgctcca cgcagataat agcccatttc ataataaaga taacacgaaa ccatttgttt
     2341 tacaagtcaa cccgctggtt tatttacctt tttttaagat tccgattcca ccccgtattg
     2401 ataaaacgaa ccattcgtgt tacaatacct tccaaaaaat acttccggtt gcgaatcgat
     2461 atttcatcac aattttagcg accgatgaat aatacctctc ccgaccgtct accgctcagg
     2521 gcatattgat actccctacg ctccattatc ttcacttccg ccttatactt ccgtagcaac
     2581 ccaatgagct cgccaacttt gggaatcccg tccgcccggt atgaaacaac caggatactc
     2641 tcttgaaacc tggcaaaaag tttgtcaaag gcttcataaa tcgaatcctt ccgaatccag
     2701 gccgagtcac ccccctttaa tctgcgatgt ttggatgaat aatcaatcag cgaaccccaa
     2761 acatcgtatt gggccaagcc ttctaaaaaa tgataaaact gataataatt gacccccact
     2821 ccttttccgg aaagatacgg cggatcgata taaaccagat cgaaactccc ttcgatctga
     2881 aaaacgtctt ggttcagcgc ggtattggcc aggccgttgc agaaaacggc gttactcgcc
     2941 gcagacgcaa atttccggaa aagcagctca cagggtgcat cccaggttgc cttatttccg
     3001 aaattccggg gaacatccga gaaacgcagg tatagatttt tgcggtgaaa cagattgaaa
     3061 gggcgtttgg ccagacaggt ttggaagagc gcaaaaaaag ccagcgcctt ccggaaagga
     3121 tcggtaaggc atccgatatt ggtcaccacc atatcgatcc aacggttctc cgcatcggtg
     3181 aaataaatat catgaaaagt gtcctgaata aatgtgggat aaaaacgccc cggataaggt
     3241 tggagcagcg catcaagctc atcatcggaa agcactaccc ggtcattttc gatcaacgcc
     3301 cgtccgatat gccagttaaa tttgagtaga tcattatagg ttacctgttt gtgatggagt
     3361 ttcaacaggt aacctatgct gccggtgcct ccgaaagcat cgagcgccgt atcaaactcc
     3421 agatccttaa ttccttccca gatccaatcc gccagtttga acttgcttcc ttgaaaccgg
     3481 gttgccggga acctcggctg ggttccggct tttccagccg gtatatcccg taatacttcc
     3541 tccatgagtg cgcatccttt attgatatga aatattcaac cgccggtttc cgggatcgga
     3601 ctggttaaag ccgactgtaa ggcgtcccac tcccggatcg ctaccggcaa cttggtgcaa
     3661 acccggccca cttcgtcgag gcgatgggcg tcggatgacc gaatcagacg ggcgccggca
     3721 agatgggggt aatcccgcgt cagtcgcgcc ggatcggcct gggccgagat ctcaactatc
     3781 cgtaaggaac gctcttccgg aatgcggttc aaattagcca ggaccgagaa actgggacgg
     3841 tcaatatggg ccggataaac caaaccgcca tactccggca cccgttgtaa cacctcatcc
     3901 caagcgagcg tcgtggagtt caaaaggagt ctatgttcct ctcccgtaac ctctccggcg
     3961 gcattgaaaa gatattgatg gccgaaacgt tcggaccggt tcggcagatc cggcaaatgc
     4021 cgatcgacaa aatcttgcca ttcccaagcc gcggccatcc cgggaaaaag acaaataagg
     4081 tggacgccct cccgggtttg gagttccatt cccggtatga tccaaagatc cggactgtcc
     4141 caattcaaaa aagccaaaca gttgcgtacc gaattatgat ccgtcagcgc catgatatct
     4201 aagccgaccc gccgggcttg agcggtaacg gcggccggag tcatccgatc atcggcgcat
     4261 ggcgacaagg cgctatggat atgaaggtcc gccaggcaat aaccgctggc cggcatctta
     4321 aaaacccgcc ttgcataaaa gggcaatcgc ctcaaacgta gtgtagggcg tggtcataat
     4381 gaccatacca ttctcgtttc ccttggtgat aaccgccggt tcgggccggt tccctccggt
     4441 gattacgatt ccccccagat ccagtagcac agccactgcc ggcacgttaa gatgggtttg
     4501 tacagtaaac cataactgac cggcctgcgc atgagccatt acctcactga gcagatcgga
     4561 ggcatatccg gaaaggaccg gcttgtccag ctgatcccgg cccgagtgta ctgcggcttt
     4621 cagaacattg gctacttctt gaagagtcat tgcttctcac ccgattcctg cgtactcgtc
     4681 cccatggcgg gcggttttcg ctccgccagc agaagtaatt cctgagcgtg ccgggacaat
     4741 tcttcacgca atttaaatac gcagtcgaag atctgcgcct tgtccagaac aatatcctca
     4801 gccagcgccc ggcagttcgg cgagccgcac gagccgcagt ccagtccggg cagggaacga
     4861 accgtagcct cgatcaactc cactttttcc atggcggagc ttagatcctg atccaggcgt
     4921 tgaaacggtt ttggaattac ctgataatcc caatcaaaaa aacgggtgtt gtaatatgta
     4981 tccgcttgag ccgcaagtgc cggatcgggg ggaggcagcc agccgtcgct aagcaattga
     5041 ttgattctca tccgggcggt aaaagaattg atcaccgtca atgccccacc cacacaacca
     5101 ttgttgcagg cctgaacctc cacgtaatct agatcattca accgccccat ctcgatctcc
     5161 tccaggagat ccacaacctg atgaattcca tcgacagcca acagattacc gattcccacg
     5221 gccctgctct cgccgccggc tccggcccat ccggctccga tcttgctaag ccgggatgag
     5281 atcgtgatgg aatccgattt tgggattagt ttttgaaggt ctccgtaaat ggcacggatg
     5341 gaaagggccc cgtttatgac accgggctct atcccatagt ggtttttgat catggtaatc
     5401 ttcgccggac agggtgaaat gaaaaagact ccaatctcac cggaacgggc cccggtctgc
     5461 ttcatgatct ccatccgggc cagtttcgcg gcggaaataa gcggcggctc aatgggcgcc
     5521 agttgctgga tcaggccggg aaaacggatc tgaatcagac ggactacggc cggacaggaa
     5581 cttgaaataa ggggtcgcgg ttccgcgtga taacgttcct ttagataacg ctttagggcc
     5641 acggtggtga gatctgcccc ccagcccgct tcggccactc ggtcaaaccc caaaccgagc
     5701 aatgcctcca aaatttgctc gggacggacc cccttttgaa attgtaccat taaagcaggg
     5761 gcaggtatag ccacccgata tctgtatccc gtcgtcgccg ccaggctgtc gccttcggcc
     5821 tgcttggcat ggttctcgca gatccggata cattcaccgc agtcgatgca ccgctcccca
     5881 tcgatactgg ctttaccgtt acgcacccga atcgcctcgg tagggcaacg tttaatgcaa
     5941 ttggtgcaac ccttgcaacg ttcattaatc aatgaaactg catgaaacca agtttccacc
     6001 ggagtccccc ttccattccg gctagtcaag ggtaaaatcc atggtgaccg tggtgccctt
     6061 cccgactgta gagatgatcg tcaactggtc cgaacaacgt ttcatattgg gaagtcccat
     6121 cccggaaccg aaacccaaac ggcgcgcgaa ttccggtgct gtagaatacc cttccttcat
     6181 cgccagatca atatccggga tgccggggcc cctgtcattg gcgacgagtc gcatgcgcac
     6241 gcgcgcgtcc gtcaaatccc ggccgattcg taaggtcagt tccccttcat aggcgtgaat
     6301 cacgatattc atctcggcct catatgcggc gatggcgatt ctgcgaacca attccatggc
     6361 aagccccaat tggatgagaa gctttttgat ctttcccgcc gcttcaccgg aggcctcgta
     6421 atcacctccg gcaatcggat agatccgttc cattgggcgg aagatgcctc cgggcggaaa
     6481 gatccaactc aaaacagcca tacctcccca aacactttcc ggaatttccg gctaagcttg
     6541 cagtctccat acgatttcaa ctatgactat caaatcccta tatacgaacg aaaaatgcat
     6601 catagcgatt tcagtttatc tgcctgatgc tcacgcgggg catattgacc ttgttcttaa
     6661 aaaaaaacga aaggcctaaa aaataggcca atctatattt acgcaacttc ctgttgaaaa
     6721 atggcaccaa cccctgcaaa tgttccgacc ggatttgagg gtttaatttt gatcgattga
     6781 aaccttatac cccttgccga cgagttcatt cttcaaccga tcggcgttgt tctgcgccga
     6841 aaaagcgccg acctgtacag tgaagggagg ggctgttccc acaaaaaccg gatatcccat
     6901 cgactgtagc tgcgttgagg ccgccagcgc ctgggtccgg ttggcgtaag gacctacccg
     6961 gactttgtac ttgacgctaa cactgggttg aactgccaca ttatccagcg tatcattggc
     7021 caccggctgg gatagatcgg aattcaccgt atcctgtgta acggtatcgg ttttttcgtt
     7081 cgacagatcg acggtttgca gggtgctatt ctcggaaccc actggcgtgc ccggttgctc
     7141 tttgacccag ccttcaaaca gcagaccaaa aactacaccc aaagaaatag ccaaaaatga
     7201 gaagaagatc aaaccgactg tcttcgataa caaattggtg atggtttttc cgcctttact
     7261 gctcttgttt tccatacgaa ggcaccccct caaaaattga accctctaca tctctttccc
     7321 aacaggccaa ttcatcagcc ccgcgatggg aggcttcaag tcaaacaaac ccggacaact
     7381 tccggaagat gaattatgat ccgttgagat ttcattgtct tagtaaagaa tactactaaa
     7441 tcaaaaaaat attcctgttt tgagaatact ttgcgtttaa atctatcagg gaggacagca
     7501 gccttcctgt gaatgaggac cttgggctcc cgtaaccatt aaccttggga taaacttgcg
     7561 aaacaataac cctcgtagat ctactctata cattataaac tccagccttt gaaaatacca
     7621 tcaccctgat gcggacaatt gcccagcata cgccgtggag atttctagca tgattatccg
     7681 gttttcttaa aacccaaagg gtgaaggcat aaaaaaaagc cgaacgacat cttatcgttc
     7741 ggttttagcg gtgactttgg cagcaacgat ttgggacagg aataaaattc gcaaggctat
     7801 tcaatttggt cctgctcacg gatttctacc cggcgtatct tgccgctgat ggtcttgggc
     7861 agctcattga caaattcgat gatccgcgga tatttgtaag gcgcggtcac ccgtttgacg
     7921 tgttcttgaa gttcctgttt caaagcttca ctagcctgat attgtttggt caagactatc
     7981 gttgccttga ctacctggcc ccggagggga tcgggtaccg cggtaacggc gcattccagc
     8041 accgcgggat gttcgagcag tgcgctctcc acctcaaaag gaccgatccg gtagccggaa
     8101 ctctttatca cgtcgtcggt ccggccgacg aaccaaaagt atccgtcttc atcacgccac
     8161 gccgtgtcgc cggtgtgata cagtccatca tgccagacgc tccgggttcg ctcgggatca
     8221 ctgtaatacc cgtcaaacat ccccaccggt ttctgacgat cggtccggat tacgatctcc
     8281 ccgatctcgc cgacttcgca ggaacggccg ttttcgtcgt aaatatcgat gatatcgatc
     8341 tcgtatccgg cagcgggttt acccatcgag ccgggcttgg gctccatcca gggaaaagta
     8401 gccaacacca cggtgagttc ggtctggcca tacccttccc gaagcttaag accggtttgt
     8461 ttcaaaaact gattatacac ctcgggattg agaggttccc ctgccacaac gcaatactct
     8521 aaattggaca ggtcaaattt ggttaaatcc tctttgatca aatagcggta aatcgttggc
     8581 ggagcgcaga atgttttgac cttataatgg gcaacctttt ccaatagatc atggggaata
     8641 aatctgtcga aatcgtaaac aaagatcccg gcaccggcca gccactggcc gtaaagtttt
     8701 ccccaagccg ctttggccca gccggtttcg gccacagtca ggtggagtcc gttttcccga
     8761 atcgactgcc aaaataaagc cgtggtgatg tggcccaggg gataaacgaa gttgtgctgc
     8821 accatcttgg ggtaaccggt agtccccgaa gtgaagtaaa gtaacatgat atcgtcgttt
     8881 tgcggcagct ccgccgccgc aggtttggca aattcctcag ccgcctcctt cagtccccga
     8941 tcaaaactga tccatccgtc cctttcaccc ccgaccgcca gcttataccg gagggttggc
     9001 gactcggcct gggcagcgtc gatctccgcc atgatgtccg gctcagccac ggacacgatc
     9061 actttgacac tggccgcctg attccggtat acgatatcct tcttcttcaa caggtgtgtg
     9121 gcagggatcg caatggcgcc aagtttatga agggccagga tgctaaacca gtattcgtag
     9181 cggcgtttca gaatgagcat cacccggtcg cctttaccga tccccagcga ccggaaaaag
     9241 ttggccgcct tatcactgta ccttttcatt tcggcaaagg tataattggc gcttgctcct
     9301 ttatcgtcgc accaaacaag cgctatcttc tgaggttcat gttccgccca ggcatcgacc
     9361 acatcgtagc cgaagttgaa atgatccgga accttgaccc ggaagttggc cgcgaagtct
     9421 tcataagatt caaaccgagt accggtcaca tattgctccc acatgtcctt tacctctttt
     9481 caataaggtc tataagatta ccgccagaaa acgggcgttt tttccattca aagccttcat
     9541 cccatgcgcc acgccggaat cataaaacag tgaatcccct tcattcagta ccaccgcgtg
     9601 gccgttgagc atcacctgga gagtgccctc caaacaataa tcgaactcct ggccgggatg
     9661 actgttgtaa tggatcggtg ctgtttcagg ccccggttcc accgtcacca gaaacggttc
     9721 agccttctta tggacaaaat tagcagccag gctttgatat ttataatcgc ggcgccgttc
     9781 caccgagacc cctttcccgt tccgcgtcaa tgaataaaga tgcagtcgcg gttcctctcc
     9841 ggtcaagaac gtggccagtt cgacatgata atattgcgcc agcttataaa ggatccctac
     9901 cggtatatcc gtttcgccac tttcatatgt ttcatactct gcacttgtta tcccgagttc
     9961 ccgggctatt gtatccggtt ccaccgcctt aatctctcgt agcccccgga ttcggtccgc
    10021 aatctgtttc aactcttcct gcatgcatta atcctccttt aaaatttcgc tgcttcgatc
    10081 gccacttgaa aatcccgatt ggattacgta tcaaaaaagt atttccgatt cgcacttttc
    10141 catgataaaa aaaaaatatg tcatacatat aatatgtatt acatattcaa caagtccatt
    10201 cctgaatcct cttttttacc aaaatatttt tggatatgtc ccccatgacg gttgatccgg
    10261 tatctccatg aagctttaat cgacagtcaa ccactcccgc ccaaaaaggg tgaagagtga
    10321 agagtgaaaa attttagtac caaaatattt gattgtaaaa acaagtaccc aatataatca
    10381 aaacattgat tgtccggtat tgtccgctag ttttatgggg tggttgtcgg gattcgatca
    10441 tagattaaaa atgaatgcgc catggggttt aagttatcga cggctccctc tgtttccgag
    10501 gaaagccgcc aatcccgggg atcaatctcc gggaaataac ggtctccggt aaacacatcc
    10561 cggattaacg tgatatataa ccgccggacg tacggaagaa acgcagtata gacttgggtc
    10621 ccgccgatga tgaaaaccgt tgtgtcgggt cgggcttcgg cgtacttcag cacttcttca
    10681 atagaccgca ttatccggca tccttgtgcc tgaaactcat ggccacgggt aagcaccagg
    10741 ttctcacgat ccgggagcgg tctgccgata ctttcaaaag ttatcctgcc cattaccacc
    10801 gggtggccta tggttaaccg tttaaagtag gcctgatcgt cggggagtct ccagggcagg
    10861 ctacgttcat taccgatcaa ccggttcaag tccattgctg cgataatcgc catcattcga
    10921 tttatcctca cctatctcgc gccttttcta aaaaggcgac tcttccctgc agggaagagt
    10981 aaccctttgg cttcgaggca caaagtcaaa agtccgagag tcaaaaatcg taagtctaga
    11041 agcactcacc cccgtcgggg ctgtcttaaa agcaaaacaa gttattttac attgaacctt
    11101 gaaaaaagaa taatcaccca accatggcag ctttcttagc tagattatgt gcaatacaga
    11161 gtaaaccaag ttcaatattg actttggtta accctcg
//
LOCUS       DASVMB010000463        11424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_55572,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000463 DASVMB010000000
VERSION     DASVMB010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55572"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF531_13010"
     CDS             complement(<1..215)
                     /locus_tag="VF531_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714926.1"
                     /translation="MNRKWIIGIIILLTLALSATALAATLSGDLELDTALSELNATAH
                     LDFDSFQTDISLSYNLKESQVEYFATQ"
     gene            complement(474..1364)
                     /gene="safA"
                     /locus_tag="VF531_13015"
     CDS             complement(474..1364)
                     /gene="safA"
                     /locus_tag="VF531_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SafA/ExsA family spore coat assembly protein"
                     /protein_id="HEX7714927.1"
                     /translation="MSDELKDNLAGAEAMPSFSAAPVCPGGQLYTVKPGDSLFFIAKQ
                     FNIPLQSLINANPQITDPNTIFPGQVICIPVPGPGITCPGGQIYRVVRGDSLYEIARR
                     FGVTLDALIRANPQISDPNLIYPGQEICVPVPGTAPCPSGNMYTVQPGDTMYEIARRN
                     NLPLSVLIAANPQITDPNLIFPGQVICIPLPQAQLPEVSIPIAPLPAMPLPAPVPTPL
                     PAPLPLPLPMPQPCPPSVMPRPCPPPVMPQPCPPQAMPFATGPSSMMYPMPVYIVIPW
                     DECPHRQGKKGHNRHGKPCR"
     gene            complement(1660..2409)
                     /gene="kduD"
                     /locus_tag="VF531_13020"
     CDS             complement(1660..2409)
                     /gene="kduD"
                     /locus_tag="VF531_13020"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEX7714928.1"
                     /translation="MILDQFKLNGKVALVTGASTGIGQSICLGLAEAGADVVGVDYVE
                     MPETHKLVESTGRRFVGVVANLMSIEPIPGVMHQALRYFGHIDILVNNAGIVRRADAL
                     DFTEKDWDDVMNLNVKTVFFCSQAVAKQFIRQGNGGKIINIASIHSFQGGIQLSSFTA
                     SKSGILGITRLMANEWARYQINVNAIAPGYMLAGDRFDEYHSPDLLNRIPAGRWGQPE
                     DMKGAVVYLSSAASSYVNGYTLAVDGGWLAR"
     gene            complement(2677..4083)
                     /locus_tag="VF531_13025"
     CDS             complement(2677..4083)
                     /locus_tag="VF531_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714929.1"
                     /translation="MKDKGIKFGFVGCGQGGGKIVDLFGQKGYKTLAINTSPVDLAAL
                     TMIPNEYRVCIGSRLGAGKDPRVGQEAIRSDAEKVKKVLQDYFARDVDFVFVVACLGG
                     GTGTGVVADTIELTRQVGLTTGCITTIPLASESLEEHKNALKGLDILSDVSPTPLYLI
                     DNELLRLKFGNRPVMGFWPMANEAIVNTLDEVNQIPNRPSNMFVFDREDFFRLLNTPG
                     CFTTLKIELEIPEGNDPTKLATICRQEIVRLLAENNFQGESIRVAALLVCPENYRVNA
                     ESVELLYQECQNLTNTPTLFRGLYIDRALQRKMVLHLLLAGLEVPLNRLQGMRTKIQE
                     LSPEIINRVNRRVSTNLGDDLVEWDQSNPLPAARRRSFGSGAQSHTPPAATGNTDLKQ
                     PLNTSPTSAASVSGPNQVPASARTEEAATQEQQEAYKILTVGQIADLMESANKISSDE
                     KDKNNKGFWNQFRSLKKH"
     gene            complement(4450..5364)
                     /locus_tag="VF531_13030"
     CDS             complement(4450..5364)
                     /locus_tag="VF531_13030"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX7714930.1"
                     /translation="MKKIKKVIVFGSINMDLVVSVDEFPKPGETVLGKQHHWFPGGKG
                     ANQAVAIRRLGGDVCMIGKIGSDNLGEAAIVNLQREGINIQKVRRVDSLTGLAVINVN
                     SQGENDIVVSPGANLVWDETDDELRRLLTGYSIVVLQLEIPIYVIERILKIAKEVNVF
                     TVLNAAPVDTGIIPSLHNVDLLIVNEIELSVLSGVAITCSAMEDALQKLSVSCKNIIV
                     TLGKDGCALWDHRIYRIPAYHVKAVDTTAAGDAFVGAVVSALSEGIDLMEASRWASAV
                     GALTVTKLGAQASIPYRNDVLDFIKMNA"
     gene            complement(5415..6386)
                     /locus_tag="VF531_13035"
     CDS             complement(5415..6386)
                     /locus_tag="VF531_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX7714931.1"
                     /translation="MKKIIIDTDPGIGALGADIDDGLAIVMALNSEKLQVLGLTTVNG
                     NVTCDQGTINALNLLEVMNRKEVPVCKGADRPLVGDMKFVRETFAEILDRAGWKEPLP
                     VKASTLQPRPEHAVDFIIDQVCRYPGEVTMVCIGPLTNLALAIRKMPQLPELIHETII
                     MGGGWTQPLDCITPVAEFNMFCDPEAAKIVLHSGMPLYMTGYELCKKTQLKPEHLAIL
                     GQRQTPTSQFIARMAEPWLAFMKAAYGLNLCFLPDPQAIALAIDPGLFTIEAMYIDIE
                     TKGELTRGMTVADRNQALHKCPYPPNVHVCTDIHVERFMELLLGLLK"
     gene            complement(6404..7732)
                     /locus_tag="VF531_13040"
     CDS             complement(6404..7732)
                     /locus_tag="VF531_13040"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX7714932.1"
                     /translation="MELMEWLSFEQVAYDELIQAREEGREVDGLEAELKTLKNKKGDD
                     HTGKLRDLYERVIHAKIRTDFPYTEPLSLEEIQKERPRISSPKMKCNFTKEELRDRIY
                     GAWLGRCAGCLLGKPVEGMSYDQIQSILKWKHTLPLNFYIAQKGGLPPELIMDQEQEK
                     RYRTGTFENVHQMLRDDDIDYTMIGLLILENYGLGFTSFDVGVTWLQTMPLLMTYTAE
                     RVAYRNFANRINPPASGYTMNPYREWIGAQIRADIWGYINPGKPEAAAELAFRDAVLS
                     HSKNGVYGELFFAAAIATAFAVNDIPSVIQGGLSVIPAHSRLFEAVQRVMEWSEVDTD
                     WQVTLKRIFEHYGNYNWFHTIPNAAIIIMALLHGGGDFERTITIAVSSGLDTDCNGAT
                     AGSIIGAFLGASRLPSKWIEPLQDHVSTAVVGMSDSSIHQLAERTVAFVR"
     gene            complement(7755..8645)
                     /locus_tag="VF531_13045"
     CDS             complement(7755..8645)
                     /locus_tag="VF531_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714933.1"
                     /translation="MQSKMEYDVLREHKNSIKINEIQWKVFVYLLLVLYTFFVIAPVL
                     VSFISSIRPGEDIFKNIMPFNWHTLIPSRVTVDAYIILFRDYSFGRVLINSFLVAVAT
                     VVGGILINAMAGLAFAIFRFRGRNILFLFVLITFMVPFEAISIPLFQLINNLGLVNSY
                     WALILPGLANGLVIFLFRQFFMGLPITLFESALIDGANWFTIFTKLALPLSRPAALSA
                     GILLFITQWEAFFYPLLVANKPEYRVIQVAIANFFTQYQTLWNLLFAAVVIAAIIPIV
                     LLLPLQRYYVQAITQSGIKE"
     gene            complement(8683..9546)
                     /locus_tag="VF531_13050"
     CDS             complement(8683..9546)
                     /locus_tag="VF531_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7714934.1"
                     /translation="MRVRLRVLLMFLIPALLGLFLFKFYPILLAAKESLFTDSFISGT
                     RKFVGLANYKLLFGDETFLNALKVTLWFNILVNPLQVILAILLALMLNQKVRGINFFR
                     SLFYLPVAIAVPIASVIWGILLNPNQGFVNSFLSFFGIPAQPFLVNQSQALYCIILIV
                     SWIGVSYWMIYILAALQEIPAELYEAARVDGASGFNVFAKITLPLIKRAIAFIAVGAT
                     TSNFLMFAPAYILTRGGPSGSTNLLMYEAYMSAFSYSDMGRSSAIVMFLLLIILLVVL
                     LEMRLLRDNEA"
     gene            complement(9624..10898)
                     /locus_tag="VF531_13055"
     CDS             complement(9624..10898)
                     /locus_tag="VF531_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7714935.1"
                     /translation="MKFAKHLSFVLLVGIMISAGVLFSVSAEKTTVTFVTLQDLIPNL
                     QPVVNAYEKENPNVNVKLEGYPFNQLFEVIETKMKAHSNDVDILNVDAPLITNYSVKG
                     YLEPLDSYFGKASKDEFLKSTITAASYKGKFMAAPLNSSSVGMYYNVDLFKKYGVAEP
                     SLNPEKRWTWEEVVAAAQKLTHSSEGIWGLAFDQFSQPYQMLPLAQSLGGRGVSPNGL
                     KASGYLNAKPWIKAAQFYQDLFQKYKVSPLGVAAFQSPELFKSGKVAMLVTGPWHVNN
                     FKSAQGLHWAYAPHPYFAGGKAVTPTGSWHVSISKNSLHKKEAADFVKFLTLREGNQI
                     YFDGDGNLPANKYTLQYAQKQATTQGNQVMSLAIYEAEHTAMARPSTPGYLEWETAVA
                     QAFEDIRNGGDPKKVLDKAAQQIDLTLAKYGR"
     gene            11276..>11424
                     /locus_tag="VF531_13060"
     CDS             11276..>11424
                     /locus_tag="VF531_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7714936.1"
                     /translation="MATIYDIAAKLGVSTATVSRAINGTGRVSEETRLKVKAAIEEMG
                     YQPNH"
BASE COUNT         3125 a         2819 c         2525 g         2955 t
ORIGIN      
        1 atttgagtgg cgaagtattc tacctgggat tccttaaggt tataacttaa gctgatgtcg
       61 gtttgaaagg agtcaaaatc caaatgggct gtggcgttta attcggaaag ggcggtatcc
      121 agttccagat ctccggacaa cgttgcagcc aatgctgtgg ctgagagagc aagggtcagc
      181 aagatgataa tcccgataat ccatttgcga ttcattgatg aaacctccgt ttaggtattg
      241 cttatatttc catggaaatt attctccaag aaatatactt ttttatgata tttctattcg
      301 cttttggctt ctcttttcct ttcaaacctc tcgtattcat atcaaccaaa aaatgccaca
      361 gtcatctcaa ccatgtttcg caccggaatt ttggatatgg gaaaaccccg gaccaaaatc
      421 cggggtttcc ggtattcgag tgtgtattgt tcatcgtgca ggcgcttttc cgactaacgg
      481 cagggtttgc catgtcgatt atgccccttt ttaccttgcc ggtgcggaca ttcatcccag
      541 gggatcacga tgtagaccgg catcgggtac atcatggaac tgggtcctgt ggcaaacggc
      601 atggcctgcg gcggacaagg ctgaggcatt accggcggcg gacaaggccg aggcattacc
      661 gacggcggac aaggctgagg catcggcagc ggcagcggta acggcgcagg caacggtgtc
      721 ggtactggtg caggcaaagg cattgccggt aacggcgcaa tcggtatgga aacctccggt
      781 aactgggctt gaggtaatgg aatgcatatc acctggccgg ggaagatcaa attcggatcc
      841 gtgatttggg ggttggctgc gatcaacacc gatagaggca ggttatttct tcgagcaatc
      901 tcatacatcg tatcaccggg ttgcacggta tacatattcc cggatggaca aggtgccgta
      961 ccgggaaccg ggacgcaaat ctcttggccg gggtagatca agttgggatc gctaatctgg
     1021 ggattagccc gtatcagggc atccagggtg actccgaacc gccgggcgat ctcatacagg
     1081 gaatcgccgc gcactacacg atagatctgc ccgccggggc aagtaatacc gggacccgga
     1141 accggaatac agatgacttg cccgggaaag atagtgttgg gatcggtaat ctgcgggttg
     1201 gcattgatca agctttgcag tgggatatta aactgtttgg cgataaaaaa taaactgtcg
     1261 ccgggtttaa ccgtatataa ctgaccgccg gggcagacag gcgccgcaga aaaagatggc
     1321 attgcttcgg cgccggccaa gttatccttt aattcatcgg acattttttt tcctcccttg
     1381 ctaaagtggc ttttgaccat atcctaaaat aaaccttttc gatggctgac ttatgcacgc
     1441 ggtcatcggg atggtggcca tttcaataac cgctcctcgt tctcttttgc agcattccaa
     1501 gacgactgct aatgtaacat attcaaaagt ccgatctttg gccaccgggc tattggcatg
     1561 atttggaggg agggagaatg aaaatcgcaa taaaaaaaac agcctgtctt ccctggctgt
     1621 ttttgatgtt ttagtcgggt ttagcgaatt ccggctgact taccgcgcca gccaaccgcc
     1681 gtccaccgca agagtataac cattcacata actggatgcg gcagaagaaa gatagacgac
     1741 cgcgcccttc atatcctccg gctggcccca gcggcctgcc ggtatccggt tcagtagatc
     1801 cgggctgtga tattcatcaa acctgtcacc cgccagcata tatccgggcg cgatggcatt
     1861 cacgttaatc tggtatctgg cccattcgtt ggccatcaac cgggtgatac ccagaatacc
     1921 gcttttgctg gcagtaaagg aggagagctg aatgcctccc tggaaagaat ggattgatgc
     1981 gatgttaatg atcttgcctc cgttaccctg gcggataaac tgcttggcca cggcctgaga
     2041 acagaagaag acggttttta cattgaggtt catcacgtca tcccagtcct tttcggtaaa
     2101 atccaaggca tccgcacggc gaacgatccc ggcgttattc acgagaatat cgatatggcc
     2161 aaaataacgc aacgcctgat gcatgactcc cgggattggt tcaatactca tcaaattggc
     2221 aaccacgccg acaaagcggc ggccggtgga ttccaccagc ttatgggttt ccggcatttc
     2281 aacgtaatcc accccgacca cgtcggcgcc ggcttccgcc aaacccaggc aaatactttg
     2341 accaataccg gtactggccc cagtaaccag agccactttt ccgtttaatt tgaattggtc
     2401 aagtatcatg gtgtcaatcc tccatcccaa cgctttaccc ggtttatccc ggaagaaatc
     2461 caagatgtaa cgacctctac tctcaattct tataatttcg ttaaattcct aaaaaatcct
     2521 gcaagatttc tatgtagatt aagaattttt caggattatt gtaaggtaac tacaagaaaa
     2581 ccggcggtcg gcttacgccc gtgccgggcg tacatataaa aactcccgct ggtggcggga
     2641 gtatgaggat aaactcataa taaggttcca gtttttttaa tgctttttca aagaccggaa
     2701 ctggttccag aaacctttat tgtttttatc cttttcatca ctggagattt tgttggctga
     2761 ctccatcaga tcggcaattt ggcctacggt caagatttta taggcttctt gttgttcttg
     2821 ggttgcggcc tcctctgttc ttgcactagc cgggacctga tttggcccgg atacggaagc
     2881 agcggatgta ggggatgtgt ttaatggttg cttaagatcc gtgtttccag tcgccgcggg
     2941 cggggtatgg gattgcgccc cggacccaaa actacgccgt ctcgctgcgg gcaacgggtt
     3001 ggattgatcc cattcaacca ggtcatctcc aaggttggta gagacacgcc ggttgacccg
     3061 gttgataatc tccgggctaa gttcctgtat tttggtacgc attccctgca aacggttgag
     3121 cggtacctca agaccggcca agagcagatg gagcaccatt ttgcgttgta aagcccgatc
     3181 gatatataac ccgcggaaca gggtgggtgt attcgtcaaa ttttggcact cttgatacaa
     3241 cagctccacg gactccgcat tcacacggta attctcgggg cagaccaaaa gtgccgccac
     3301 ccggatactc tcgccctgga agttgttctc cgcaagcaag cgaacgatct cctgacggca
     3361 gatagtagcc agttttgtgg gatcattgcc ttcgggaatt tccagctcaa tcttgagtgt
     3421 ggtgaaacaa ccgggagtat taagcagacg gaaaaaatcc tcgcggtcaa agacgaacat
     3481 attggaagga cggttcggaa tctggttcac ctcatccaag gtgttgacga tcgcctcgtt
     3541 ggccatcggc caaaatccca tgaccggacg gttaccgaac ttcaaccgga gtagttcgtt
     3601 atcgatcaga taaagcggtg tcgggctgac atcacttaga atatccaagc ccttcaaggc
     3661 atttttgtgc tcctccagcg attcgctggc aagcggaatg gttgtaatgc aaccagtggt
     3721 caagcctacc tgccgggtga gttcgatggt atcggccacc acaccggttc cggttcctcc
     3781 gcctaaacag gccacgacaa aaacaaaatc gacatcacgg gcgaagtaat cctgcaaaac
     3841 cttcttgacc ttctctgcat cggagcggat agcctcctgg cctacccgag ggtccttgcc
     3901 cgcacccaac cgggaaccga tacaaactcg atattcattg ggaatcatcg tcaaagcggc
     3961 caaatccacc ggagaggtat tgatggctaa agttttatat cccttttgcc cgaaaagatc
     4021 gacgatcttg ccgccgccct gtccgcatcc tacgaatccg aacttaattc ccttatcctt
     4081 catgaaccgg ttacctccaa tagcctcgga tagcgaggga gatataagta tgcaaaacat
     4141 tacatgtaca atttgactta tttctacaaa atcctttttt tctgtaacag aacaattgca
     4201 taattcgaca tcttccaaaa aaaccgtcgc taatctctga tttcattgta atggagaaag
     4261 agtatttgcg caactgtctt tctacggaaa agcttcccta tgtgcttttt ccggatagga
     4321 tttccgattc cattgatcag atcgctatta cctattacag gtttgacttt accgaaaaat
     4381 atagccaacg gtgatgccgt tggcaaaaaa atttctactc tttcaggaac cattcaacaa
     4441 atctgttatt tatgcgttca tcttaataaa gtccaagaca tcgttacggt aagggatcga
     4501 agcctgagcc ccaagtttcg ttacagttag ggcccccaca gcgctcgccc aacggcttgc
     4561 ctccataagg tcgattccct cgcttaaagc ggaaaccaca gccccaacaa atgcatcacc
     4621 ggcagcagtt gtatcaaccg cttttacatg ataagccgga atacgataaa tcctgtgatc
     4681 ccataacgcg caaccatctt tgcctaaagt gacaatgata tttttacatg aaacggaaag
     4741 tttttgaaga gcgtcttcca tcgcggaaca ggtgatcgcc actccgctta aaaccgataa
     4801 ttcaatttca tttacgatta ataaatcaac attgtgcaaa gaagggataa tgccggtatc
     4861 aaccggagca gcgtttaaaa ccgtaaaaac attgacctcc ttggcgattt tcaatattcg
     4921 ttcgataaca tatatgggaa tctccaattg taaaacaaca atcgaataac ccgttaatag
     4981 cctccgcaat tcatcatcag tctcgtccca gaccaaattg gctccgggag aaacgacgat
     5041 atcattttct ccctgggaat taacattgat tacggccaag cctgtcagac tatcaacccg
     5101 tctaaccttt tgaatattga tgccttcacg ctgcaaatta acaatcgccg cttctcccag
     5161 gttatcgctt cctattttcc cgatcatgca gacatctccg cccagtctac ggattgctac
     5221 ggcctgatta gcgcctttcc cccctggaaa ccaatgatgc tgttttccca agaccgtctc
     5281 tcccggtttt ggaaattcgt ccactgacac caccaaatcc atattgatac tcccaaagac
     5341 aatgactttt tttatcttct tcaccttctc aaaaggatgg tttgtaaatc ggtttttaga
     5401 gacccccagg ggatttattt taataaaccg agtaacaatt ccataaagcg ctcaacatga
     5461 atatccgtac aaacatggac atttggcgga tacgggcatt tatgcagcgc ctggtttcga
     5521 tcggcaaccg tcatgccccg ggtcaattcc cccttcgtct caatatcaat gtacatcgcc
     5581 tcaattgtaa agaggcccgg atcaattgcc agggcgatag cttgcggatc cggtaaaaaa
     5641 caaagattta acccataggc ggctttcata aaggccagcc agggttcggc catccgggcg
     5701 ataaactggc tggtcggagt ctggcgttgt cccaaaatag ccagatgctc cggttttaac
     5761 tgagtctttt tgcacaattc ataaccggtc atatagagag gcattccgga gtgaagtacg
     5821 atttttgcgg cttccggatc acaaaacata ttaaactcag ccaccggggt gatgcaatcc
     5881 aaaggttggg tccatccccc gcccatgatg atcgtctcat gaatcaactc cggcaattgc
     5941 ggcatctttc ggatagccaa cgccaggttg gttaaagggc cgatacagac catcgtcacc
     6001 tcacctggat atcggcatac ctgatcaata ataaaatcta cagcatgctc cggccggggc
     6061 tgcaatgtac tggctttaac tggcagcggt tccttccaac ctgcccgatc taaaatttca
     6121 gcaaaggttt ctcggacaaa tttcatatcc cccaccagag ggcgatccgc tcccttgcaa
     6181 accgggactt cctttcgatt catgacttcc aagagattta gcgcatttat cgttccctgg
     6241 tcgcaagtca cattaccgtt caccgtagtg agccccaata cctgtaattt ttcggagttc
     6301 aaagccatca cgatggccaa gccgtcgtcg atatccgccc ctaatgcgcc gatacccgga
     6361 tccgtatcga tgatgatctt tttcataatc atgcctcctt aggttaacgt acaaaagcca
     6421 cggttctctc ggccaattga tgaatcgagg aatccgacat ccccacaacc gcggttgata
     6481 catgatcctg caacggttca atccatttgg agggcaaacg tgaggccccg agaaatgccc
     6541 cgatgatcga tccggcggta gccccgttac aatcagtgtc caagccggaa cttacagcga
     6601 ttgtgatagt tcgttcaaaa tctccaccac cgtgtaataa agccataatt ataatcgctg
     6661 cattcggaat ggtatgaaac caattgtaat ttccatagtg ttcgaagatt cgcttcaagg
     6721 taacttgcca atccgtatcg acttcagacc attccataac cctttggacc gcttcaaaaa
     6781 gacgggagtg cgccggtatc actgaaagcc ctccctggat aactgaggga atatcgttta
     6841 cggcaaacgc tgtcgcgatc gctgccgcga agaataattc accataaacc ccgtttttgg
     6901 aatgagataa aacagcgtcc ctaaacgcaa gctcggcagc tgcctccggt ttaccgggat
     6961 taatataacc ccaaatatcc gcgcgaatct gtgctccgat ccactcacga taaggattca
     7021 ttgtataccc tgacgcgggt ggattgatcc ggttggcgaa attgcgatag gcgactcgtt
     7081 cggctgtata cgtcattaat aatggcatcg tttgtaacca ggttacaccc acatcgaatg
     7141 aggtaaaccc cagaccgtaa ttttcgagga ttagaagtcc gatcatcgta taatctatat
     7201 catcatcccg caacatttgg tgaacattct caaatgtccc ggtacgatac cgtttctctt
     7261 gctcctgatc catgattaat tccgggggta gcccgccctt ttgcgcaata tagaaattca
     7321 acggtaaggt atgcttccac ttaagtatgg attgaatttg atcataagac atcccttcca
     7381 cgggtttacc caataaacat ccggcacacc ggcccaacca tgctccatat atccggtccc
     7441 gtaattcttc cttggtaaaa ttgcacttca ttttcgggga agatatccgc ggccgttctt
     7501 tttggatctc ctccaacgag aggggttcgg tatatggaaa atccgttcgg atcttagcat
     7561 ggatgacccg ttcatagagg tctcggagtt taccggtgtg atcgtctccc tttttatttt
     7621 ttaaagtttt cagttccgct tctaaaccgt ctacttccct tccttcctcc cgggcttgaa
     7681 tgagttcatc ataagctacc tgttcgaaag agagccactc cattaattcc attgtttcac
     7741 ctcatgaagt tttcctattc tttaataccc gactgggtaa tcgcttgaac ataatagcgc
     7801 tgcaaaggta ataacaaaac gatgggtatg atcgccgcaa tcacgacagc ggcgaataga
     7861 agattccaaa gagtctgata ttgggtgaag aagttggcga tcgccacctg gatcacccgg
     7921 tactccggct tattcgccac caataacgga taaaagaacg cttcccattg agtgatgaat
     7981 aatagaatcc ccgcgcttaa cgccgccggc cgggatagag gcaacgccag cttggtgaag
     8041 atcgtgaacc agtttgctcc gtcaattaag gccgactcaa atagagtgat tggcaatccc
     8101 atgaaaaact ggcgaaacaa aaaaataacc aatccgttgg cgaggcctgg taaaatcaaa
     8161 gcccaataac tattgaccaa gcccaaattg ttgattaatt gaaaaagcgg gatcgagata
     8221 gcctcgaaag ggaccataaa agtaatcaat acaaataaga ataatatgtt tcggccacga
     8281 aaccggaaga tggcaaaagc taacccggcc atcgcgttaa tcaggatacc tccgactacc
     8341 gtagctactg caaccaagaa actattgatc agcactctcc cgaaactgta atcccggaaa
     8401 agtatgatat aagcatccac tgtcactctg cttggaatca atgtatgcca gttgaatggc
     8461 atgatatttt taaagatatc ctctccgggg cgaatcgagg atatgaaaga gacaagcacc
     8521 ggcgcaatga caaagaaagt atataaaacc aatagaaggt agacaaaaac cttccattga
     8581 atctcgttta ttttgatgct gtttttgtgt tctcgtaata catcatattc cattttactt
     8641 tgcaaagcct tttctccttc ccgtttctga tcggcagcca aattaagctt cgttatcccg
     8701 caggagcctc atctcaagga gaactaccag aagaataatg agtaacagaa acataacaat
     8761 cgcgctcgaa cgccccatat cggaataaga gaaggcactc atatacgcct catacattaa
     8821 aagattggtt gaacccgaag gtccaccccg ggttaaaata taggccggag cgaacatcaa
     8881 aaaattggag gtggtagccc ctactgcaat gaatgcaatg gcccttttga ttaaaggtag
     8941 ggtgattttg gcaaaaacat tgaaacccga tgccccatct acccgggccg cttcatatag
     9001 ctcagcgggg atttcctgaa gcgctgccaa aatataaatc atccaatagc ttactccaat
     9061 ccaactgacg ataaggataa tacaataaag ggcttgactt tgatttacta aaaacggctg
     9121 agcgggtatt ccaaaaaatg aaaggaagct gttgacaaat ccttgattgg ggtttaacaa
     9181 aatcccccaa ataacggaag ctataggtac tgcgatggcg accggcaaat aaaacaggga
     9241 acggaaaaaa ttaatccccc gaaccttttg attcaacatt agggccaata agatagccaa
     9301 aataacctga agcggattga ctagaatatt aaaccataag gttaccttca atgcattaag
     9361 gaacgtctca tccccaaaaa gcagcttata attggcgaga ccgacaaatt ttctggttcc
     9421 tgaaataaaa gaatccgtaa agagactctc cttcgccgcc agaagaatcg ggtagaattt
     9481 aaacagaaac aacccgagca aagcggggat tagaaacatt aacagaactc tcagcctaac
     9541 cctcacgata ctactccttt caccgcgatt gttccgaaaa acggggatga agcaccgatt
     9601 gatgcctcat ccctccaaat tcattaacgc ccgtatttgg ccaatgttaa atcgatttgt
     9661 tgcgcggctt tatcaagaac ttttttcgga tcgccgccgt ttcgaatatc ttcaaaggct
     9721 tgggccacag cggtctccca ctccaagtat cccggagtgg aagggcgagc cattgcggta
     9781 tgttccgcct catagatcgc caaagacata acctgatttc cctgagttgt agcctgcttt
     9841 tgcgcatatt gcaaggtata tttattcgcc ggaagattgc cgtccccatc aaaataaatc
     9901 tgatttccct cacgcagggt taagaatttt acgaaatcag ccgcttcctt tttatgcagg
     9961 ctattcttgc tgatactaac atgccaactt ccggtgggag tgactgcctt gcccccggca
    10021 aagtagggat gcggtgcata agcccaatgc aaaccctggg cactcttgaa attattgaca
    10081 tgccaagggc cggtgactaa catggcaact ttgccgctct taaacagttc aggagactga
    10141 aaagcggcta cgcccagcgg gctgacctta tatttctgga aaaggtcctg atagaattgg
    10201 gcggctttga tccaaggctt ggcattgaga tagccactgg ccttcaaacc gttggggctg
    10261 actccccggc cacccagtga ttgagccaaa ggaagcatct ggtatggctg actgaattga
    10321 tcaaacgcca gaccccagat cccttcactg gaatgagtta acttttgggc tgccgccact
    10381 acttcttccc aagtccatcg cttttccgga ttcaatgaag gctctgctac accatatttc
    10441 ttgaaaagat ccacattgta atacatgcct acactagaag agttgagtgg agcggccata
    10501 aattttcctt tatatgaagc cgcagtaatc gttgatttca ggaattcatc cttgcttgct
    10561 ttcccaaaat aggaatccaa tggttcaaga taacctttca cactgtagtt ggtgattaga
    10621 ggcgcatcta cattcaagat atccacatca ttactgtggg ctttcatttt tgtttcaatc
    10681 acttcaaata attggttgaa aggatacccc tccaatttga cgttgacatt gggattttcc
    10741 ttctcatatg cattgacaac cggttgcaaa tttggaatta aatcctgtaa tgtaacaaat
    10801 gttacggtcg ttttttcggc actaacagag aatagtaccc cggctgaaat cataattcct
    10861 acaagtaata caaaagacaa atgcttggca aatttcatga caatcctcct ctatgttgtt
    10921 gaagtgattg gaaattcttt cttgcgtaag atgttacata aagatcacct acccggccgg
    10981 cgaaaaattg atgaataatt ccagctaaaa tactttatta cccggcagac agttgtaacg
    11041 aataataaaa ctgagtaacc ggttactcat attattcttc tctccggtgt taattcctgc
    11101 attaatttct gtactgaaaa gaaaatatta ttcccctttg gcaggattaa gcttaacatg
    11161 agcagaatat aatttttgaa ccttcttgga caggtaaccg gtttcttttt gaacaaattg
    11221 cttctagcta agaattaata ttaaatactc caataagttt tcaggaggaa cagttttggc
    11281 tacaatttac gatatcgctg caaaactggg ggtttcaacc gcgactgttt cccgggcgat
    11341 taacgggacc ggccgggtga gcgaagagac aaggttaaaa gtaaaagcgg ccattgagga
    11401 aatggggtat caacccaatc atat
//
LOCUS       DASVMB010000464         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_719149,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000464 DASVMB010000000
VERSION     DASVMB010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719149"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1856
                     /locus_tag="VF531_13065"
     CDS             393..1856
                     /locus_tag="VF531_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714937.1"
                     /translation="MEITKVFKPRYVLLVLAVIAVLVGGLSVMAAGGKWVTGDFHTHT
                     FLTDGNNMQEEVVKNAFVSGLDWMANSEHGGTSQKDPKGKAYPAPVWRWITIKDASFP
                     IIQSLRNNYPNKLLIQGLEWNVPSHEHASVGIVGTEPGPVSDFEYMFDGSDTDTSRAA
                     ENLAKINKVHADAVAGAKWLETNYPNTSYFLLNHPSRKLAYSVADIRDFIDAAPDVCF
                     GMEGFPGHQKEKNRGGYGNDLGANTYKARTYGGADYMLAKVGGLWDALIGEGRHFWIF
                     VNSDYHSSDNDFWPGEYAKNYTWVTGSDYNSLIDGLRSGNSFAVQGELINALDFNAAA
                     NNEQATMGQALDIAANSNLVITIRFKSPQYNNNGDAVKVDHIDLISGEVGAKELPSSP
                     EYSKDTNETTKVIARFTAKDWKLQNGYYIIKYQIKNVGKNMYYRLRGTNLGLNVTNET
                     DKDGNPLNDDLVGTNDRVKAYKDLWFYSNPIFVSVTK"
     gene            complement(1939..2685)
                     /locus_tag="VF531_13070"
     CDS             complement(1939..2685)
                     /locus_tag="VF531_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7714938.1"
                     /translation="MKEARKSKVGFVLIPGGGMSDWLWKKLEGYLNFPFIQIDRRLKN
                     NTHQNRLNASFSDCLDYIGEKIQERNFERIILVGHSGAGVLAAKAAVSFQENVVHVVY
                     VAANIPKTGTTVIDALPESVQEKNIAAIKQQAPLDKIPLKMLEGVFRSAFCNTCSEED
                     IDYLLQQDFLPEPLCAVTEKMTWDNFPDLSQTYIILTDDHTLPVEKQKEMAANLNIND
                     LRMIDSDHLVMISHPRELAAILNEIAGNYQ"
BASE COUNT          741 a          609 c          631 g          742 t
ORIGIN      
        1 tgaatcgtga aaacgcttca ttgattcgcg aaagaacctt gctgtgtacc cgcggtgatt
       61 ttgaaaggat gcgaccgcag gttataatcg aaaaaatcgg tgtttgatac ttaaaacggg
      121 taccggcatt ttaggggatg cggcggcatc atttaaacca cattgggttt ggtgattatc
      181 ggtgtgatta ataacgcttt attatgaatc cagacataaa ttgagaaaaa gccgggagct
      241 tcccggcttt ttctttttga ttttgaagga ccataatttt ccttgaataa agcgatggag
      301 tttacgattt aatcagcatt taaacagggc ataaaggagc actaaaaata gaccgctatg
      361 atttggttaa aatcagttga aaggagatta ttatggagat cacaaaggtt ttcaaaccgc
      421 gttatgtact tctcgtcttg gcagtaattg ctgtattggt cggcggtttg tcggtcatgg
      481 cagccggcgg caagtgggta accggagatt ttcacaccca tacttttttg acggacggca
      541 ataacatgca ggaagaagta gtcaagaatg cgtttgtttc cggacttgac tggatggcca
      601 actccgaaca cggcgggact tcccaaaaag accccaaggg taaggcgtat cctgctccgg
      661 tatggcggtg gattaccatc aaagacgctt ctttccccat cattcaaagc ttacgaaata
      721 actaccccaa taaactattg attcaaggtt tggaatggaa tgtcccatct catgagcatg
      781 ccagcgtagg cattgtggga accgaaccgg gtccagtcag cgattttgaa tatatgtttg
      841 acggcagcga caccgacacc agccgggccg cggagaatct ggccaagatc aataaggttc
      901 acgccgatgc cgttgccggc gcgaaatggc tggaaaccaa ttatcctaat acaagttact
      961 tcctgctcaa ccatccatcg cgtaaacttg cctattccgt agcggatata cgggatttta
     1021 tcgatgcagc ccccgatgta tgttttggta tggaaggttt cccggggcat caaaaagaaa
     1081 agaatcgcgg cggttatggt aacgatcttg gtgccaatac atataaggcc cgtacctatg
     1141 gcggcgccga ttatatgctt gcgaaggtag gtggcctttg ggatgcctta attggtgaag
     1201 gccgtcattt ctggatcttc gtcaactccg actatcattc ttccgataac gatttttggc
     1261 ccggtgaata cgccaagaat tatacctggg ttacaggaag cgactataac tcgctcatcg
     1321 acggtttgcg ttccggtaat tcctttgcgg ttcagggcga gcttatcaat gcccttgatt
     1381 tcaatgcagc cgctaataat gaacaggcca caatgggtca agccctagat attgctgcga
     1441 actccaattt ggtgattacg attcgcttta agagccctca atataataac aatggcgatg
     1501 cggtcaaagt ggaccacatc gatttgatct ccggtgaagt cggagccaag gaactcccaa
     1561 gcagcccgga atatagtaaa gacaccaacg aaaccaccaa agtcatcgcc agattcaccg
     1621 caaaagattg gaaattacaa aacggttatt atatcatcaa atatcaaatc aaaaatgtcg
     1681 ggaagaatat gtattaccgg ctacgcggta ccaatctcgg tctgaacgta accaatgaga
     1741 ccgataagga cggtaatccg ctcaatgacg atttggttgg aaccaatgac cgggtcaaag
     1801 cctataaaga tctttggttc tattccaacc ccatctttgt ttcggtaaca aaataaagtt
     1861 ggaatgccca tatttgaaag gctgctcgcc agagcagcct tttttacttg cgtttagcag
     1921 agtgcaacct aacggttttt attgataatt tccggcaatt tcgttgagta ttgcggcgag
     1981 ttcgcgggga tggctaatca tcaccaggtg atcgctatcg atcatccgta gatcgttgat
     2041 atttaaattt gcagccattt ccttttgttt ttccaccggc agggtatgat catcagtaag
     2101 aataatatag gtttgactca ggtccgggaa attatcccat gtcatttttt cggtaaccgc
     2161 acacaacggc tccggcagaa aatcctgttg caaaagatag tcaatatcct cctcggaaca
     2221 ggtgttacaa aatgccgacc ggaagactcc ttcgagcatt tttaggggaa tcttgtcaag
     2281 aggggcctgt tgtttgatgg ctgcgatgtt tttctcctgc accgattccg gcagggcatc
     2341 gattactgtt gttccggttt tcggaatatt ggcggcaaca taaactacgt ggaccacatt
     2401 ttcctgaaac gaaacagccg cttttgccgc caagactcct gcgccggaat gcccgaccag
     2461 aatgatccgc tcaaagtttc tttcctgaat cttttcgcca atgtaatcaa ggcaatccga
     2521 aaaactcgca tttaaccggt tttgatgggt gttatttttt agcctgcgat caatctggat
     2581 gaaaggaaaa ttcaaatagc cttcgagctt tttccacagc cagtcggaca ttccgccgcc
     2641 ggggatcaag acaaacccaa cctttgattt tcgagcttcc ttcatataaa tgctcctatt
     2701 tcagcctctt tacagagatt tct
//
LOCUS       DASVMB010000465         5911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_719151,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000465 DASVMB010000000
VERSION     DASVMB010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719151"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF531_13075"
     CDS             <1..383
                     /locus_tag="VF531_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7714939.1"
                     /translation="EILVLDEPTTGQDYRESLQIMDLVAELNRMGHTVIFITHDMQLV
                     ASYAGRVIMMGKGRVLADGTPREVLPRTDLLAATFLKPPQITQLAQALNDCGFAGDAI
                     RVEELVTVFREKTGTGRHRLAGVG"
     gene            399..1202
                     /locus_tag="VF531_13080"
     CDS             399..1202
                     /locus_tag="VF531_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX7714940.1"
                     /translation="MGNAVEYLDVASPLHKLNPLTKLLGLLCIFLMGVIFSDPFILML
                     LVGLILLLFLLAKAGGTFLKYLRVMLAAGAVMLLLQLLFSHDGPALYRFIPAGIPVLS
                     WMGTINRDSLVLGIVMVVRMNVFGLSLPLLLATTQPRDLVLVMVERLKFPYQYALMFI
                     TALRFIPTLFVEIENIFQAQQARAFELTTRNPLKRVKAYFPLVVPLVLIALRKAEQLA
                     LAMETRGYGMGKRTYLRRPVFGAADFGACAGCISLVLLAVTLRVYGVAV"
     gene            1581..3425
                     /gene="iorA"
                     /locus_tag="VF531_13085"
     CDS             1581..3425
                     /gene="iorA"
                     /locus_tag="VF531_13085"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX7714941.1"
                     /translation="MAKQLLMGNEAIARGAIEAGLNFAAAYPGTPSSEIIGSLADWAV
                     TDQNNGTAPYYVEWSVNEKVAMEAAAGAAYAGAYSLVAMKQVGLNVASDPLMSLAYIG
                     IEGALVVVVADDPGPHSSQTEQDTRSFARYANLPVLDPASPEEALEMTKAAFELSHRY
                     HLPVLLRPTTRVCHATAGIEPAAVTVNRPVGFVKDPNWVIFPKLSYLKHQQLEKIQTQ
                     LAAEFGNSPQNQLIQGDRVGIVSGGVADLYLREAVALLGIHPTILKIGTPYPAPVQLI
                     QRFLGMVDRVIVVEELDPVLEEAVHSAAGTSGFHGPIHGKLSGDLPYAGEYNVDLIRT
                     TLTKILKPDDEVREVAPTSAAPTLPVRPPVLCAGCPHRASFYAVKQATKGTDAIYTGD
                     IGCYTLGNAAPLEMVDTCLCMGAGLTIAQGLYRVNPGRPVIAFIGDSTFFHTGIPGVL
                     NAVYNNHAIKIMLLDNGTTAMTGHQPHPGIGRNAVGLPAPVIDPAAILKACGVQFVEV
                     VDPLHLKQTEATIRRALEFPGVAAVVLRHPCISLNRSKGKRLFRILPERCTGCRRCLK
                     ELGCPAFYREATSEKAGTGGIQIGPSCTGCGLCSEVCASGAIEEVVKG"
     gene            3422..4018
                     /locus_tag="VF531_13090"
     CDS             3422..4018
                     /locus_tag="VF531_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX7714942.1"
                     /translation="MKDRLDIIISGVGGQGTILASRVLAQAALNAGFTAGTAETIGMA
                     QREGSVQSHVRIGSPAFGPLIGRHGADVLLGFEPAEAQRACPLMKPDGIGLVNLHPIH
                     PVTVALDSTAYPLEEIKSYLCALPVRMTFINAFQLANVAGNFRTVNAVMLGALAGTGI
                     LPVPKLELLKVLLELVPERARDVNERAFELGFEACKDQ"
     gene            4311..5633
                     /locus_tag="VF531_13095"
     CDS             4311..5633
                     /locus_tag="VF531_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX7714943.1"
                     /translation="MMNRGSTAVIPEEYEDLHDPEFTRLAGKDAANRLDRSLREFLER
                     IHKNSAFYRKKLAAAGFKPQDFKGVKDLAALPFTTKEELREIYPLGLQAVPDRDVIRI
                     HSSSGTTGKPIIIPYTRKDVADWAVMFARCYAFAGVTRGDRMQITPGYGLWTAGIGFQ
                     AGCERLGAMAVPMGPGNTEKQLQMMQDLKSTVLCSTSSYALLLGEEVAKRNLRDKLYL
                     RIGIIGSERWGDKMKARIEEALGIETFDIYGLTEIYGPGIGIDCQYHEGIHYWSDYLY
                     FEIIDPNTGAVLPEGEMGELVITTLTKEGAPLLRYRTRDLTRIIPGHCRCGSVYPRID
                     RVLGRSDDMIKIKGVNIYPGQIEDVLKETQGASSEYQIRLTRVEARDSMLLRVEGEPG
                     ADPTQLKERIVQNFKKRIGVIIEVEVVPIGALPRSEKKSKRVFDERDI"
BASE COUNT         1327 a         1447 c         1699 g         1438 t
ORIGIN      
        1 ctgagatatt ggtgctggat gaaccgacca ccggacagga ttaccgcgag tccctccaaa
       61 tcatggattt ggtggcggaa ctgaatcgca tgggtcatac cgtgatcttc atcacacacg
      121 acatgcaact ggtggcgtca tacgccggcc gggtgatcat gatggggaag ggccgggttt
      181 tggcggacgg gactccccgt gaggtgctcc cgaggactga tttgctggcc gcgacctttt
      241 tgaaaccgcc gcagatcacc caattggccc aagccctgaa cgattgcggt tttgccggcg
      301 atgccatccg ggttgaggag ctggtaacgg tttttaggga gaaaaccggc accgggcggc
      361 acagactggc cggcgtcggg taacaggaga agaaggtaat gggaaacgca gttgaatatc
      421 tggatgtcgc atcaccgctt cacaagctga atcccctcac caaattgctg ggacttctat
      481 gcatcttcct gatgggggtc atcttttcgg atccgttcat cctcatgtta ctggtgggat
      541 tgatcctgct cctttttctg cttgcgaaag cgggcgggac ttttttaaaa tacctccggg
      601 tgatgcttgc ggccggggct gtcatgttac tactgcaatt actcttctct catgacggtc
      661 cggcattata ccgctttatt cctgcaggta tcccggtctt aagctggatg ggaacgatta
      721 atcgcgatag tcttgtgctg ggaatcgtga tggtagtccg aatgaatgtc ttcggccttt
      781 ctttgccgct attgcttgcc acaacccagc cccgggattt ggtgctggtc atggtggaac
      841 ggttgaagtt tccgtaccaa tatgctttaa tgttcataac agccttgcga tttattccta
      901 cgctttttgt ggagattgaa aatattttcc aggcccagca ggccagggcc tttgaattga
      961 ccaccaggaa tcctttgaag cgtgtcaagg cttatttccc cctggtagtg ccgctggtgc
     1021 tgattgccct gagaaaagcg gaacagcttg ccctggccat ggagacccgt ggttacggaa
     1081 tggggaaacg gacctattta aggcgccccg tctttggcgc ggcggatttc ggcgcctgtg
     1141 ccggttgcat ctccctggtg ttactggcag ttacgctccg ggtgtacgga gtggcggtgt
     1201 aatataggca acagggaaca ggcaataggg aaaagggatg aggcgtgagt agcgcgcccc
     1261 ctggggcgcg aagcaataga gaaagataga aaggacatag gtcggcttcg tcatctacga
     1321 ctcactctcg ctctctctaa cggcaagcgc cagagagagt aacccggatt cttcgaggct
     1381 ttttttcacc tcaattaatt taccttcttc cctcttaccg cttggtggta gagagtgcgc
     1441 gtccattgga cgcgagagca agtagggtga gtcgcttcta gactttcgcc aagagtaaac
     1501 ctcttacaat actcttgcct gttccctgtt gcctattgcc ttaattgact tcggacttat
     1561 tttatatgag gtgaaatgtt ttggccaaac aacttctgat gggaaatgaa gcgatcgccc
     1621 ggggcgctat cgaggcggga ctgaacttcg ccgcagcata ccccggaact ccttcgtcgg
     1681 agatcatcgg atctctggcg gattgggccg ttacggacca gaataacggt accgcaccat
     1741 attatgtgga gtggtcggtc aacgagaaag tggccatgga agcagctgcc ggcgccgcat
     1801 atgccggggc ttacagtttg gttgccatga agcaggtggg gttgaatgta gcaagcgatc
     1861 cgctcatgag cctggcatat atcggaattg aaggggcatt ggtggtcgtt gtggccgatg
     1921 atccgggacc ccattcttct caaaccgagc aggatacccg gtcttttgcg cgctacgcca
     1981 acctcccggt gctggacccg gcctctccgg aagaagccct ggagatgacc aaggctgctt
     2041 ttgagctttc ccaccgttac catttgccgg tactgctccg gcctaccacc cgggtttgtc
     2101 acgccaccgc cggaatcgag ccggcggcag tgactgtgaa ccgacccgtg ggctttgtca
     2161 aggatccgaa ctgggtcatc ttcccgaaac tttcatatct caagcaccaa cagcttgaga
     2221 agattcaaac gcaattggcc gccgagtttg ggaatagtcc tcaaaatcaa ttgatccaag
     2281 gggaccgggt gggaatcgtc agcggcggag tggctgatct ttatcttcgg gaggctgtgg
     2341 cgttacttgg gattcacccc acgatcctga agattgggac gccttatccg gcaccggtcc
     2401 aactgattca acgattttta ggcatggtgg atcgggtgat tgtagtagag gaactcgatc
     2461 cggtgctaga ggaagcggtt catagcgcgg ccgggacctc cggatttcat gggccgatcc
     2521 acggtaaact cagcggggat ttgccttatg ccggagagta caatgttgac ttgatccgaa
     2581 caactttaac caagatactg aagcccgacg atgaagtcag ggaagttgcc ccgacttccg
     2641 cggcccctac gttaccggta agacctccgg tgttatgcgc cggttgcccc catcgggctt
     2701 ccttttatgc tgtcaagcag gctaccaagg gaacagatgc catctatacc ggggatatcg
     2761 gctgttacac cctgggtaat gcggcaccgc tcgaaatggt cgatacctgt ctttgcatgg
     2821 gcgcaggact tacgatcgcc caggggttat atcgggtaaa cccgggcagg cctgtcatcg
     2881 ctttcattgg tgattccacc ttctttcata ccgggattcc cggtgttttg aatgcggttt
     2941 acaataacca tgccatcaag atcatgctgc ttgacaacgg caccaccgcc atgacgggac
     3001 accaacccca tccgggaatc ggccgcaatg ccgtgggatt acctgcgccg gttattgatc
     3061 cggcggcgat cctcaaggct tgtggagtgc agttcgttga ggttgtcgat ccgttacatt
     3121 tgaagcagac cgaggccaca atccgccggg cccttgagtt tccaggcgta gcggctgtgg
     3181 ttttacgtca tccttgcatc agtttgaacc gttccaaggg gaaacggctt ttccggatcc
     3241 ttcccgaacg ctgcaccgga tgccggcgct gtctcaaaga actgggctgc ccggcctttt
     3301 accgcgaggc aacttcggaa aaggccggaa ccggtggaat ccaaattggc ccatcctgta
     3361 ccggttgcgg gctttgcagc gaagtttgcg cctcgggcgc catcgaggag gttgttaagg
     3421 gatgaaggac cgtttggata tcatcatctc cggagtggga ggccagggaa ccattttagc
     3481 ctcccgggtt ctggcccagg cggcactcaa cgccggattc acggcgggta cagccgaaac
     3541 catcggaatg gcgcaacggg aaggttccgt ccagagccat gtccggattg gttcccccgc
     3601 tttcgggccc cttatcggaa gacacggcgc cgatgtgctt ctggggtttg aacccgccga
     3661 ggcgcaacgc gcttgccctc tgatgaaacc ggacggcatc gggttagtca atttgcatcc
     3721 gattcacccg gtaactgtgg cgcttgactc cacggcttat cccctcgagg agatcaagtc
     3781 atatctttgc gccttgccgg tccggatgac attcattaat gcctttcaac tcgctaacgt
     3841 ggcgggtaat ttccggactg ttaacgcggt aatgttgggc gctctggcag gtaccggcat
     3901 tctacctgtt cctaagttgg aattattgaa ggttcttctg gagttggttc cggagcgagc
     3961 gcgagatgtg aatgaacgcg cttttgaact tggttttgaa gcttgtaaag atcagtaaag
     4021 tgatgaatgg catgctctaa tggcaaaatc gttaataatg atggaaacat tatctattta
     4081 cggcaaattt taatagccga caagaaatgg cagtcaagat taacgatttc tacgaataga
     4141 catatttttc ggagtttagt ataatgaaaa agtaaaaccg gagataagct gggtatttta
     4201 agatggaaat tcattgtgaa attccagaat ttcactgtgg aaagagtctt atcttaaaat
     4261 attccagatt cctatcaatc ttgcccatag tatagaggag gtaagccgag atgatgaacc
     4321 gcggttctac ggcggtaatt ccagaagagt acgaggactt acatgatccg gagtttaccc
     4381 ggttggccgg aaaggatgca gccaatcgac tggacagatc gttgcgggaa tttttggaac
     4441 ggatccacaa gaacagtgcg ttttatcgaa aaaaattggc tgcagccggt tttaaacccc
     4501 aggattttaa gggcgtaaag gatttggcgg cattgccgtt tacaaccaag gaggagttgc
     4561 gggagattta tcccctgggg cttcaagccg tgcccgaccg ggatgtgatc cggatccatt
     4621 catcctcagg gactaccggg aagccgatca tcatccctta cacccggaag gatgtagccg
     4681 attgggcagt gatgttcgcg cgttgctatg cctttgccgg cgtgacgcgg ggggaccgga
     4741 tgcaaatcac cccgggttac ggcctttgga ccgctggcat cgggtttcag gccggctgcg
     4801 aacgcttggg ggctatggcg gttcccatgg gtccgggtaa taccgagaag caactccaga
     4861 tgatgcagga tcttaagtcc accgtcttgt gcagtacctc atcatacgct ttacttttgg
     4921 gggaagaggt tgccaaacgg aaccttcgcg acaagctcta tctgcggatc ggaatcatcg
     4981 gctccgaacg ttggggcgat aagatgaagg ctcgtatcga ggaagcttta gggatcgaga
     5041 ccttcgatat ttacggcctg actgagattt acggccccgg tatcgggatt gactgccaat
     5101 atcacgaggg aattcattac tggtccgact acttgtactt cgagatcatc gacccgaaca
     5161 ccggagcggt tctacctgag ggagagatgg gggaactggt tattaccacg cttacaaagg
     5221 aaggcgcacc tttgcttcgt taccggaccc gggatctgac ccggatcatc ccggggcatt
     5281 gccgttgcgg gagtgtttac ccacggatcg atcgggtgct gggccggagc gacgatatga
     5341 tcaagattaa gggcgtcaat atttacccgg gacagatcga ggatgtacta aaagagaccc
     5401 agggggcttc gagcgaatac cagatccggc tgacccgggt tgaggcccgc gattccatgc
     5461 tgctccgggt cgaaggcgag cccggcgcgg atccaactca actcaaggaa cggatcgtcc
     5521 agaacttcaa gaagcgaatc ggggtcatta tcgaggtgga agtggttccc ataggcgccc
     5581 tgccgcggag cgagaagaag tcgaagcggg tctttgacga gcgggacatc taaaaagtgt
     5641 gaagggtgaa gtgtgaaggc caaggagaga tccttggctt ttttgttgca ggttatcaga
     5701 ggaaagggca ggaagcttta aaatattata gaaattgtaa aatggatagt cgccatgaaa
     5761 aatctccgta aagcgcggtc aatcagtggc gtcgatgaaa aatgtttatg aggtgttgat
     5821 gatgaaggca aaaacccgga taatgatggt gattgttttt ggtgtaattg tcgcttttgc
     5881 cgttggtttt cgttatgttg attacttttc c
//
LOCUS       DASVMB010000466         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_204148,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000466 DASVMB010000000
VERSION     DASVMB010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204148"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF531_13100"
     CDS             complement(<1..317)
                     /locus_tag="VF531_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7714944.1"
                     /translation="MAKLYFRYSAMNAGKTTQLLQVKYNYEERGQNVLLLKPAIDNRQ
                     VADQVISRIGLASDAVIIDSTIDLLELVKQAMVEHEVNCLLIDEAQFITRPQVLQLCR
                     IVD"
     gene            complement(364..1278)
                     /locus_tag="VF531_13105"
     CDS             complement(364..1278)
                     /locus_tag="VF531_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX7714945.1"
                     /translation="MKTIRHVYLSGLGAIGSAFAGKLFDLDPGCLTVLADDERIKRYT
                     RDGVAINGKHYPFHYLRPEEESVPADLILIAVKNHHLSQAIQDIRKFVREDTIILSLL
                     NGIVSEEIIGREYGLEKLLYSFVVGTDAVRKGTHTRFSNIGKIVFGERSNSFSPKVAA
                     VKELFDRAQIPYQIPEDMLRELWWKFMMNVGVNQVSAVLKAPYGVFQKVREARELMEM
                     ASNEVVVIAARAGINLSAADIEKYIMILDTLAPEGKTSMLQDVEAGRKTEVEIFAGTI
                     MDLGRRYGVPTPVNEILFRQIRVLEQAR"
     gene            1573..2049
                     /locus_tag="VF531_13110"
     CDS             1573..2049
                     /locus_tag="VF531_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX7714946.1"
                     /translation="MRQTIILSKQAFDSLTAGLSEIKGRTDDILNGFLLRPASEVNEV
                     KRVLNDYVHQLDGMIRSIITSDSASDEFPYAIAGSEVVLEDTCCQATYCYRLISPLKE
                     KIDLHEISFLSPMGQALLFKKVNDQFSVKAPGGEFVYKILSVKIAVSPEDSLVDYV"
     gene            complement(2180..3139)
                     /locus_tag="VF531_13115"
     CDS             complement(2180..3139)
                     /locus_tag="VF531_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7714947.1"
                     /translation="MKIVVLDGFTLNPGDLSWRGLEELGPLTVYDRTPEDKILERIGT
                     AEIVYTNKTPLSRETLVRAAAMKFIGVLATGYNVVDTKTAKERGIIVTNIPTYGTTAV
                     AQFVLALLLEICHHVGAHDEAVKKGDWTNNPDFCFWNYPLMELAGKTMGIIGFGRIGQ
                     ATAAIAQSFGMRVLAYDQHQNKALETATLKYVELDRLLGESDVISLHCPLFESTKGII
                     NRNSIAKMKDGIIIINTSRGPLVVEEDLAAALNSGKVYSAAVDVVATEPIEADNPLLT
                     AKNCIITPHIAWAPKESRARLMAIAVDNLKAFLAGTPVNVVNL"
     gene            complement(3186..3950)
                     /locus_tag="VF531_13120"
     CDS             complement(3186..3950)
                     /locus_tag="VF531_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX7714948.1"
                     /translation="MLKEKLMSGQKVYGTMLRIIRNPAVCLLAKNNGLDFVMYDCEHG
                     SYSMETLHDLFITGNAIGLDGFLRVPILSKDYISRALDQGAHGVMVPMLETPEMANEF
                     VKYSKYAPIGDRGYAAGAAHVNYVLGGKHSEVMEEANNKIVSIAQIETKLSVDNAEAI
                     AATPGIDALIIGPNDLSISLDIPGDLLNPIEIDAIAHVVGACKKNKKAFGLHGPAKLQ
                     EKFSDDITIVMFQTDAEVLASGFAQLKETCVNFSAK"
     gene            complement(4111..>4568)
                     /locus_tag="VF531_13125"
     CDS             complement(4111..>4568)
                     /locus_tag="VF531_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX7714949.1"
                     /translation="HLADIIQRDLGMDLRNVPGAGAAGGLGAGVIAFAKAQLKSGIDI
                     VLDTVKFEAILTGADFVLTGEGKIDGQSLRGKVVVGVARRAKTQDVPVIAVVGDIGDE
                     IEQIYSQGVTAVFSINRVAVPFEKARLRCESDLALTVDTLMRTLQMKRA"
BASE COUNT         1101 a         1184 c         1022 g         1261 t
ORIGIN      
        1 ttgtctacga tccggcaaag ctgcaagacc tgaggcctgg ttataaattg ggcctcgtcg
       61 atcagcagac aattcacttc atgctcaacc atggcctgtt tcaccagttc gaggaggtcg
      121 atggtggaat cgataatcac cgcgtcactg gcaagaccaa tcctggatat cacttgatcc
      181 gcgacctgcc ggttgtcgat ggcgggcttc aacagtaaaa cgttctggcc ccgttcctcg
      241 tagttatatt tcacctgcag caattgggtg gttttcccgg cgttcatcgc tgaataacga
      301 aaataaagtt tcgccatgat attcacctta agaaatttat cttgtgatgt ggaatgatcc
      361 gttctatcgt gcttgttcca agacccgaat ttgcctaaaa agtatctcgt tgaccggagt
      421 cggtaccccg taccttcgcc ccaaatccat gatggttccg gcgaagatct ccacctcggt
      481 ctttcgtccc gcctccacat cctgaagcat ggaggtttta ccctccgggg cgagcgtatc
      541 caggatcatg atgtacttct caatatccgc cgcgctaaga ttgatccctg ccctggcggc
      601 gataaccacc acttcattgg aggccatctc catcagctcc cgggcctccc tgaccttttg
      661 aaagacccca tatggcgcct tgagcaccgc cgagacctgg ttgaccccga cattcatcat
      721 aaatttccac cacagctcgc ggagcatgtc ttccgggatc tgatacggga tttgggcccg
      781 atcaaaaagt tccttgaccg ctgccacctt gggggagaaa gagttggacc gttccccgaa
      841 gacgatcttt ccgatgttcg agaagcgggt atgggttccc ttccggacgg cgtccgtgcc
      901 caccacaaag gagtaaagca gcttctccaa gccgtattcc ctacctataa tctcctcgct
      961 aacaatcccg ttgagcaggg aaagaatgat ggtatcctcc ctcacgaatt tccggatatc
     1021 ttgaatcgcc tgggacaaat gatggttttt gaccgcgatc aaaatcaaat ccgccggaac
     1081 gctttcctcc tccggccgta gatagtgaaa gggataatgc ttaccattga tggctacgcc
     1141 gtcccgggta taacgtttaa tccgttcgtc atctgcaagc accgtaaggc atccgggatc
     1201 cagatcaaag agtttccctg caaaggcgct gccgatggcg cccaggccgg acagataaac
     1261 gtgacgaatg gttttcataa tatggctcct tatgggaatt cactaattca ttattttacc
     1321 atagttccag gatgaaagcg acttgaccgg catgaaatgt atttacttga tccggatcca
     1381 ttttatatat gctttactag taaatggatt gtgattccat catttacaat gactgtatgt
     1441 tatgatagaa tgtttccata caggatattt cgcgtcggcg aaatgtcctt ctgctttata
     1501 ttggatggtt ttacatttta tggacaatcg tggcaattag acaaaatgat tggtttaaga
     1561 aaggagttcc ccatgagaca aacgatcatt ttatcaaaac aagcctttga ttcgttaacc
     1621 gccggtttat cggagattaa aggaagaacc gacgatatcc tcaatggatt ccttcttagg
     1681 cctgcaagtg aagtcaatga agtgaagcgg gttttgaatg attatgttca tcagctggat
     1741 gggatgatcc gtagtattat cacttcggat tctgccagtg acgagttccc gtatgcgatc
     1801 gccggaagtg aagtggttct ggaggatacc tgctgtcaag cgacctattg ctatcggtta
     1861 atctcgccgc tcaaagaaaa gatcgatctt cacgagattt catttttatc ccccatgggt
     1921 caggcgctgc tttttaaaaa agtcaacgat caattttctg tcaaggcacc ggggggtgag
     1981 tttgtttata agattttatc cgtgaaaatc gctgttagtc ccgaagattc gttggttgat
     2041 tacgtttagc gacttcctcc cgccccctct tccgcttatg cctgcaaaga ggggcgccta
     2101 tggaaagccg accggcttgg cttatgaact ttttgtcttc ttttgcagct aaactactgt
     2161 tgatattttc gttcctccac tacaggttaa ccacattcac cggagttccc gctaaaaatg
     2221 ccttcaaatt gtccacagcg atcgccatca gccgagctcg tgactctttg ggggcccaag
     2281 cgatatgtgg ggtgattata caattcttgg ccgttaacaa tggattatcc gcttcgatcg
     2341 gttcggtggc gaccacgtca accgccgcac tatatacctt gccactgttt aacgccgcgg
     2401 ccagatcttc ctctactacc aacgggcctc tggaagtgtt gataataatt ataccgtctt
     2461 tcatcttcgc aatggaattg cgattgatga ttcctttggt gctttcaaac aatggacagt
     2521 gcaaactgat tacgtcggat tctccaagca atcgatccag ctcaacatat ttcagggttg
     2581 cagtttccag cgctttgttt tggtgttgat cataggccaa caccctcata ccgaagcttt
     2641 gggcaatggc cgcggttgcc tggccaattc tgccgaaacc gataattccc atggttttcc
     2701 cggctagttc cataagagga taattccaga aacaaaaatc cgggttattg gtccagtcgc
     2761 cctttttgac ggcttcatca tgggccccga catggtgaca gatttccagt agcaatgcca
     2821 aaacaaactg ggccaccgcc gttgttccat aggtgggtat attggtgaca atgatccccc
     2881 gttccttggc ggttttggtg tcgacgacat tatatccggt ggccaatacc ccgataaact
     2941 tcatcgcagc cgcccggacg agggtctccc gggaaagcgg ggttttattg gtgtagacta
     3001 tttcggcagt tccgattcgc tccaaaatct tatcttccgg agtccgatca tagactgtga
     3061 gcgggccaag ttcctccaga cccctccagc tcaagtcgcc cgggtttaag gtaaatccat
     3121 ccaaaacaac gattttcacg ttaatttctc cttctattcg tatggctgcg caagttctct
     3181 ttttatcact tcgccgaaaa attcacacaa gtctccttaa gttgtgcaaa cccggatgct
     3241 aagacctcgg cgtcggtttg gaacataacg atggtaatat cgtcgctaaa tttctcttga
     3301 agcttggctg gcccatgcag accaaaagct tttttattct ttttgcaggc accaacgaca
     3361 tgggcgatgg cgtcaatctc aatcgggttt aacagatcac ccgggatatc gagagaaatt
     3421 gagaggtcat tcggcccgat gatgagggcg tcgatccccg gagttgccgc aatggcttcg
     3481 gcattatcca ctgacagttt ggtctcaatt tgagcgattg aaacgatctt attgttggct
     3541 tcctccatta cttcggaatg ttttcctccc aatacgtaat ttacatgagc ggctccggca
     3601 gcatatcctc tgtcgccaat cggcgcatac ttcgaatatt tgacaaattc attcgccatt
     3661 tccggagttt ccagcattgg aaccatgact ccgtgggcgc cttgatcaag ggctcttgat
     3721 atatagtcct tggaaagaat cggcacacgc aggaaaccat ccagaccaat ggcatttccg
     3781 gtgatgaata aatcatgaag tgtttccatg ctgtaacttc catgttcaca atcatacatg
     3841 acgaagtcca gtccattatt ttttgcaaga agacaaaccg caggattgcg aataattctg
     3901 agcatggttc catagacttt ttggccggac ataagttttt cttttaacat gaatcatact
     3961 ccttatatac taaatgatta ttcaaatcgg acctggattg cacctgacta tatttttgat
     4021 tgattccact tcccccattc aaagataatt ttctaaccgg ttatttatac agcaatagat
     4081 atgccgataa aaaagcgagg ctccggtata tcaagcccgc ttcatctgca gcgtccgcat
     4141 gagcgtatcc acggtcagcg ccagatcgct ttcacatctt aacctggcct tttcaaaggg
     4201 aaccgctacc cggttaatgc taaacacggc ggttactccc tgggaataga tctgctctat
     4261 ctcatctccg atatcgccga caacggcgat aaccggaaca tcttgggtct tggcccgccg
     4321 ggctaccccg accacaacct tcccccgtaa actctggccg tcgatcttgc cttccccggt
     4381 gagtacgaaa tcggctcctg taagtattgc ctcaaatttc accgtgtcga gcacgatatc
     4441 gatccccgac ttcaattggg cctttgcaaa ggcaatcacc ccggcgccca atccgccggc
     4501 ggccccggcc ccgggtacat ttctcagatc catgcccaga tccctttgga tgatatctgc
     4561 cagatgct
//
LOCUS       DASVMB010000467         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_353245,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000467 DASVMB010000000
VERSION     DASVMB010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353245"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="ald"
                     /locus_tag="VF531_13130"
     CDS             <1..1060
                     /gene="ald"
                     /locus_tag="VF531_13130"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX7714950.1"
                     /translation="GCTPAMAKDLVDAGHRVFIEQTAGNGSGFGDSEYLDVGAEIRTD
                     AATVYSESQMIIKVKEPLPLEYPLLREGQILFTYLHLADDRELTEGLLRSKSIGIAYE
                     TVLAGDGSLPLLVPMSEIAGRMAAHIGAYYLAKPNGGPGVLMQGAPGVPAAKILILGG
                     GTVGFNAAQIAGGMGGDITLLELNPDRIRFLDNALPKNVKIVMSNRYNLIAALKEADL
                     VIGAVLIPGAKTPCLVSRDMLKYMKQNSVIVDVAVDQGGCIETTRPTTHSNPTYVVDG
                     VLHYAVANMPGAFARTATIALTNATLPYALYLANLGPEKALAADAGLLKGLNAYRGKL
                     ACPQVAECHHLDYSAPSF"
     gene            1444..>2650
                     /locus_tag="VF531_13135"
     CDS             1444..>2650
                     /locus_tag="VF531_13135"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7714951.1"
                     /translation="MKQELKLDLSKLKSFVSDPELVMMGDQVKLAHGLLHNRTGAGNY
                     YLGWVDLPVKYDRQEFEAIIKAAQRIQDSSEALVVIGIGGSYLGARAAIEMLNNSFFN
                     QMDGKKRRYPQIFYMGNNISSTYTADLLDLLAEKDFSVNVISKSGTTTEPAVAFRLIK
                     ELLIKKYGVNGAKSRIYATTDRARGALKQLADAEGYTKFVIPDDVGGRYSVLTPVGLL
                     PIAASGINIQEIMDGARDGYQEFQNPDLEKNAAYRYAAARNVLYRRGKLVEILVNYEP
                     GLHYFAEWWKQLYGESEGKDHKGIFPAACDFSTDLHSMGQYIQEGRRDIFETVLWLEK
                     PRRSLTIPDDPGNIDGLNFLSGKSMDFVNQKAFEGTLLAHSDGEVPNMVVRVPEHNAY
                     NFGKTVYFFE"
BASE COUNT          640 a          616 c          727 g          667 t
ORIGIN      
        1 gggttgtact ccggccatgg ccaaggattt ggtcgatgcg gggcaccggg ttttcatcga
       61 acagacggct ggaaacggca gcggctttgg tgattcggag tatttggatg ttggggctga
      121 gatccgaaca gatgcagcta cggtttattc tgaatcgcaa atgatcatca aggtcaagga
      181 gccgctccct ctggagtatc ctttactgcg ggaagggcag atactcttca cctatcttca
      241 tttggcggat gatagggagc tgaccgaggg tctgttacgg tcgaaatcta tcggtatcgc
      301 ttatgaaacg gtcctggccg gggacggcag tttacccttg ctggttccca tgagtgaaat
      361 cgccgggaga atggcagccc atatcggggc ctattatttg gcaaaaccca atggcggtcc
      421 cggtgttttg atgcagggcg cgccgggggt tccggcggcc aagatactga tactgggcgg
      481 cggcacagta ggttttaatg ccgcacaaat cgccggcggt atgggcggag acattacatt
      541 gctggagcta aatcccgacc ggatacgttt tctggataac gctttgccga agaacgtaaa
      601 gatagtcatg tccaaccgct ataatctgat tgccgcgtta aaagaggcgg atttggtgat
      661 tggggcggtt ctcataccgg gagccaagac tccttgcctt gtatcccggg atatgttgaa
      721 atatatgaag caaaatagtg tgattgtgga tgtggcggtg gatcaaggcg gctgtattga
      781 aacgaccaga ccgaccaccc attccaatcc gacctatgtt gttgacgggg tgctacatta
      841 tgctgtggcc aatatgccgg gtgcttttgc acgaaccgcc actatcgccc ttaccaacgc
      901 gaccctgcct tatgcgttat atctggcgaa ccttggaccg gaaaaagcgc tggccgcaga
      961 tgccggccta ctcaaggggc tcaatgctta tcgcgggaaa ttggcctgcc cccaggtggc
     1021 tgaatgtcat cacttggact atagtgcgcc ctcgttttga tagaataaaa atcagccatg
     1081 ttcgaactag tggttgatcc gatactcatt ttcgtgtctt tggtgtgttt cgttgtggat
     1141 aaagttggcg tacgatagag acttttttaa ccacgaaatc accaaaatac acgaaaattt
     1201 atttgaggcg aaactcttta aacccaaaaa gaggatgatt ctccttatat agatttcttt
     1261 acgtaaggat ctttttttac acttttgcta aaattgccaa aaacatagag gaaatctaca
     1321 attcgcagcg aaccatttcg tttagttttg cataggaaaa taatttacta aacagactat
     1381 taccggttcc gggtatccgg cggagctcgg tttccggtat agccataacg gaggaatcgt
     1441 tttatgaagc aggaactaaa actggactta tccaaactga aatcattcgt atccgatccg
     1501 gaactagtga tgatgggcga tcaggtaaag ctcgcccatg ggttgcttca taaccggacc
     1561 ggcgccggga actattatct cggctgggtg gatctccctg tcaagtacga tcggcaggag
     1621 ttcgaggcga tcatcaaggc ggcgcagcgg atccaggata gttccgaggc tttggtggtc
     1681 atcggcattg gtggctccta cctgggggcc cgggctgcca tcgagatgtt gaataattcc
     1741 ttcttcaatc agatggatgg taagaaacgc cgttatcccc agatttttta catgggaaac
     1801 aatatcagtt ccacttacac cgctgattta ttggatctcc tggcggagaa ggatttttcg
     1861 gtcaatgtca tctccaaatc cgggactacc accgagccgg cggtagcttt ccggctgatc
     1921 aaggagctgt tgatcaaaaa gtacggagtg aacggtgcca agagccgcat ctacgccact
     1981 accgataggg cccggggagc gctgaaacaa ttggcggatg ccgagggtta taccaagttt
     2041 gtcatccccg acgatgtcgg cggcagatat tcggtactga ctcccgtggg tttactgccg
     2101 atcgccgcta gcggcatcaa tattcaggag atcatggacg gtgcccggga cggttatcag
     2161 gagtttcaga acccggatct cgagaagaat gcggcttacc ggtatgcagc cgcccggaac
     2221 gttctctacc gcagggggaa gcttgtcgag attctggtca attatgagcc ggggctgcat
     2281 tacttcgcgg aatggtggaa acagctctat ggtgagagtg aggggaagga tcataagggt
     2341 atcttccccg cggcctgtga cttctccact gacctgcatt ccatgggaca atatatccag
     2401 gaaggacggc gcgacatttt cgaaaccgtt ttatggcttg aaaaaccccg tcgttctttg
     2461 accatccccg atgatcccgg aaatatcgac ggtcttaatt tcttaagcgg caagagtatg
     2521 gattttgtca atcaaaaggc ctttgagggt acattacttg cccatagcga cggtgaggtc
     2581 cccaatatgg tggtgcgggt accggaacac aatgcctaca acttcggcaa aacggtctat
     2641 ttcttcgaga
//
LOCUS       DASVMB010000468         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_719414,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000468 DASVMB010000000
VERSION     DASVMB010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719414"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF531_13140"
     CDS             <1..737
                     /locus_tag="VF531_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7714952.1"
                     /translation="FLGSWRIEIRDLDRQIKVANQAVRLALHSFKNQFLALQMAMNMT
                     STQLADIDGEAVTKARTQIQWAQDICSQALLRLDVLHTQADRVHVNPQRLSLTELWKV
                     VLHDNEERLKHVVVTNQYAENVYILGDREHIQAVLENLLINACDAMAEKELPGYQPQL
                     LVEINREYEWRYIRITDNGVGISKENRHKVFRPFFSTKPAKSNWGMGLTYCHRVVKVH
                     RGFINIQSREGTGSTVEVVLRGVQKI"
     gene            1044..>1955
                     /locus_tag="VF531_13145"
     CDS             1044..>1955
                     /locus_tag="VF531_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7714953.1"
                     /translation="MSQSSQLEIGKHGFFKELTKNKALFLMLLPGALILFLYNYLPMF
                     GIVVAFKNYRIDGGFFSSLLNSEWSGFGNFEYLFRSPDAFNMIRNTLLYNIAFVTSCT
                     IIAIAFAIMLNEIYSRKLSKFYQSAMFLPYFLSWVVFSYLVQAFLSPDKGFLNMSIFK
                     SFGIKSVYWYSEPVYWPYIILLVNAWKWVGNNCIIFLASIIAIDQELYEAARIDGASR
                     WQQIIHITIPQITPVIIVMTLLFVGRIFNTDLGLFMLVPRNSGILSSVTWTIDVYVYN
                     TLIRNGDIGMSSASALFQSVGGFITILL"
BASE COUNT          505 a          420 c          459 g          571 t
ORIGIN      
        1 ggttcctggg ttcttggcgt atcgagatcc gggacttgga ccggcagatc aaggtggcca
       61 atcaagcggt ccgtctggca ctccattctt ttaaaaatca gtttctcgcg ctgcaaatgg
      121 cgatgaacat gacttcgact caacttgccg atatcgacgg ggaagcggtg accaaagccc
      181 gtacccagat ccaatgggcg caggatatct gctcccaggc cttgctccgc ttggatgttc
      241 ttcataccca agccgatcgg gttcatgtaa acccacaacg tttatccctg acggaactat
      301 ggaaggttgt tttacatgat aatgaagagc gattgaaaca tgttgtcgtc accaaccaat
      361 acgccgaaaa cgtttatatt ctgggagacc gtgaacacat ccaggcggtg cttgaaaatc
      421 tgctcatcaa cgcatgtgac gccatggccg aaaaagagct tcccggttat caaccccagc
      481 ttttggtgga gatcaatcgg gaatacgaat ggcggtatat ccgaattacc gacaatggtg
      541 ttgggatatc caaagagaat cggcataagg tcttccggcc atttttcagc accaaaccgg
      601 caaagagcaa ctggggtatg gggttgactt attgccaccg cgtggttaag gtacaccggg
      661 gattcatcaa cattcaaagc cgtgagggga ctggcagcac cgtggaagtg gttttgcggg
      721 gcgtccagaa aatctaaacg gttattttgg catgggtttg attatggtaa aagacgtcta
      781 ggaaatatcc tcctaggcgt tttgttttta cggggtgtta atagagttga aatcggatgt
      841 taacattggt tcaaatgaca tcgatgtaca agcaagcgag ggttgatcgc cataggaaca
      901 atgatattct ttactgccgt tgggacgaat tagtatatcg gtcataaatt gaacttgaat
      961 acctgctttt atcacctttt tatatccgga atcgaaggtt ggaattgcgg ggataaatta
     1021 atgcggaaga ggcgagaaag attatgagcc aaagttccca acttgaaatc gggaagcacg
     1081 gtttttttaa agaattaacc aaaaacaagg cattattcct gatgctcctc cccggagcgc
     1141 tgattttgtt cttgtataac tatttgccga tgttcggcat tgtggttgca tttaaaaact
     1201 accggatcga tggcggtttt ttctccagcc tgcttaatag tgaatggtcc gggttcggca
     1261 actttgaata tttgtttcgc tcaccggatg cgtttaatat gattcggaat acacttcttt
     1321 ataatatagc cttcgttact tcctgtacga ttattgcaat cgcctttgcg attatgctca
     1381 atgaaatcta tagccgaaaa ctttcaaaat tctatcaaag cgccatgttc ttaccgtact
     1441 ttctttcctg ggttgttttt agttatttgg tgcaagcctt tttaagcccg gataaaggtt
     1501 tcttaaatat gagcatcttt aagtctttcg gtatcaaatc tgtttattgg tactccgaac
     1561 ctgtctattg gccgtacatt attctattgg tgaatgcctg gaaatgggta ggcaataact
     1621 gcatcatctt tttggcctcg atcatcgcaa tcgatcaaga gctgtacgag gcggcccgta
     1681 ttgatggagc cagcagatgg caacagatta ttcatattac cataccgcaa atcacaccgg
     1741 ttatcatcgt aatgacctta ctttttgtcg gcaggatatt caacaccgat cttggactat
     1801 tcatgctggt accccggaac tcggggatac tatcctcggt cacttggaca attgacgttt
     1861 atgtatataa tacgctgatt cgcaatgggg atatcgggat gtcctcagcc tcggcccttt
     1921 tccaatccgt gggaggattt attacaattc tcctc
//
LOCUS       DASVMB010000469         7061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_498225,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000469 DASVMB010000000
VERSION     DASVMB010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498225"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1239
                     /locus_tag="VF531_13150"
     CDS             214..1239
                     /locus_tag="VF531_13150"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX7714954.1"
                     /translation="MKLGIRVNQWVRQFSKYRYILCGVVLCIGVIGFISYRTALDRIT
                     LKVDGTVCEWQTINATVADVLREKQVDLKPGDVVKPGLKTPVTEDLEIEVIRAFPVTL
                     KVAGVIKTYNTIAQPVKDVLAQAGVAYGPDDKVRPALDTIVQPEQTIRVIKVTSEIIT
                     RTIVTEPATEYYRDKSLKRGTQKVVRTGKSGRIERQIKIVYEDGREINRSKIAERVIQ
                     PAVKSLVAVGLPVLRTLVTNRGSYQYVEVRTMTATAYYPGPESCGKYAVNGVTYTGKK
                     AGFGIVAVDPRVIPLGTKVYVEGYGKAEAADIGGAIKGNKIDLCFETFREAALYGVKK
                     IKVYILE"
     gene            1345..2217
                     /locus_tag="VF531_13155"
     CDS             1345..2217
                     /locus_tag="VF531_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEX7714955.1"
                     /translation="MFNVDKYQDQFTYYELQDLAAGSRVKLVPERGGIVVGFSLGDQE
                     LLYLDKETLNNPEQNVRGGIPYLFPICGRLSDDEYTWNGEKYRMNQHGLARNLPWTVL
                     NQDTREEAAITLQLKGDRATRSQYPFDFELDFKYSLKGKTLRIDQEYRNCSGVELPFY
                     AGFHPYIRVAEKERLIYDISASAYLDFEDLRIKPYTGNLDLTHENQAKLFMDHAPGRL
                     SFRDPVLNRKFHYEYGPEFKYPVLWTIPGQDFVCVELFMARPDALNTREDLIFIPPHR
                     THQTFVAITAELTK"
     gene            2451..2798
                     /locus_tag="VF531_13160"
     CDS             2451..2798
                     /locus_tag="VF531_13160"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7714956.1"
                     /translation="MFEERRNLTRVNTHIVAEINVQGNPERLCGYIENISEKGIGVLS
                     LDPFIKGAEAATTFQLPGIPRSLEAKVSLLNSKSGLYNLYYHSFRFEGLSESEQMAIS
                     HYIKQQESVENPA"
     gene            complement(2889..4127)
                     /gene="fabF"
                     /locus_tag="VF531_13165"
     CDS             complement(2889..4127)
                     /gene="fabF"
                     /locus_tag="VF531_13165"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7714957.1"
                     /translation="MNRRVVVTGIGLVTPLGLTTEANWEALVTGRSGVGSITRFDTSK
                     FGTKIAAEVKGFDPGQYLEKKEIRKMDRFIQFTVAATNEALADSGLTITPANASKVGV
                     IIGSGMGGIETVCQHQDTFVTQGPDRISPFFIPSCVINLAPGQISIRTGATGPNFSPV
                     SACATGTHAVGESFHIIRRGDADAVITGGAEAAIIPLAMAGFGNMKALSTRNDAPDKA
                     SRPFDADRDGFIMGEGAGILILEELEHALHHGAKIYAEVIGFGMNSDAYHITSPAPDG
                     MGASRCMELTLQSAGITPAEVDYINAHGTSTPINDQMETKAIKHCFGEHASKLLISST
                     KSMTGHLLGAAGAIEAAYTALTLYHQTAPGTINLDHPDPDCDLNYLPNHAQKADLKVA
                     VSNSFGFGATNACVALRRFE"
     gene            complement(4236..4691)
                     /locus_tag="VF531_13170"
     CDS             complement(4236..4691)
                     /locus_tag="VF531_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7714958.1"
                     /translation="MANGEHIHQENSNRPLFCLLIKNTWQKLSRYYNQRLAGFDLTVP
                     KALLLLEISPGGGESPKSLGTKLDLESSSMTGLLNRLEKKGLIERRRDPRDRRGLLIF
                     LTPNGIIARETIKSLVNEMDHKLQGALSPDEIKVFRKVSTVIGKQLLSS"
     gene            4889..6355
                     /locus_tag="VF531_13175"
     CDS             4889..6355
                     /locus_tag="VF531_13175"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7714959.1"
                     /translation="MKLLDLKKIIPVPPKFYEVDFNGLNNDSKKIQPGELFIAVRGYK
                     TDGYIYLDEAIRRGASAAIVEADQTGPAGFPVLPVAYTRRIQGQVAAQFYGEPSRRMR
                     LIGVTGTNGKTTVTHLIEHLLNSSYIPTGLIGTVWINNGRERQYSTRTTPDSIELQRL
                     FAGMMANGIETVVMEVSSHALALERTAGSEYDVAVLTNITHDHFDFHRNYQQYMEAKL
                     QLFRNLDPAGKPGKYAVLNRDDPSSEQIASECRVPVIFYGEGDGSFSRLEAVERTGLE
                     SLIRIRLGDEECRVRTRLPGEFNIYNILAAVTVAYREGLSVEDIEAALPDFPGVPGRY
                     QEINCGQPFRVMVDFAHNPAALENILKMAGEYTRGRRILVFGCEGEKDRVKRPLMGAI
                     AAYHAEVPILTSDNVYHEDILQIFTDVLQSLSPGEQGRFIIEPDRRKAIKTAVSMANP
                     GDFLIVAGKGHEQYLVQGNQKLHFDDAEELRRLLQRTE"
     gene            complement(6511..>7061)
                     /locus_tag="VF531_13180"
     CDS             complement(6511..>7061)
                     /locus_tag="VF531_13180"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX7714960.1"
                     /translation="GLEKVFNADKSNITAVMNQSFGISDLTTEEIDAIKKTKAGSMNY
                     TVGPIISKRSGIGWTTIGHTGEEVFLYAYGPGKPAGLIENTDIAKIIAKDMGVSLADV
                     NQKLFVMAGDTGYEWGVRTLDTKYGVNRPYIILTSGNTVVELIANTNTTYVNGKPHSL
                     RNISLIVNGVPYIPREALTYLK"
BASE COUNT         1752 a         1637 c         1909 g         1763 t
ORIGIN      
        1 tcccctaagc ctgattctgt atatgttttt gctccaatta aagctgcctt ttagctaaag
       61 ctgctttgta gcgaaaagct gccttttagc ttaaaaatct ttctagcagg atttccggtt
      121 tgcgccggcg aattattact aacatattaa gaatatgttc ccagctagac accggtttcg
      181 tggtttttaa ttctgttcct aaggggggta agaatgaagc taggaatacg agtcaatcaa
      241 tgggtccggc aattctcgaa atatcgttac atactttgcg gagttgtgct gtgcatcggg
      301 gtaatcggat ttatctcata ccggacagca ctggatagaa taaccctgaa agttgatgga
      361 acggtttgtg agtggcaaac catcaacgct acggttgctg atgttttacg ggagaaacag
      421 gttgatctca aaccgggcga cgtagtgaaa ccgggattaa aaaccccggt gactgaagat
      481 ttggagattg aagtcatccg ggcctttcct gtcaccttga aggtggccgg ggtcatcaaa
      541 acctataata ccattgccca accggtcaag gatgtactcg cacaagccgg agtcgcctac
      601 ggaccggatg ataaggttcg tccggctttg gacaccatcg tccaaccgga acaaaccatc
      661 cgggttatta aggtaacttc ggagatcatc actcgcacga tcgttaccga accggccact
      721 gagtattacc gcgataaaag tttgaagcgc ggtacccaaa aggtggtccg caccggtaaa
      781 tccggtcgaa tcgaacggca aatcaagatc gtatacgagg atggccgtga gatcaaccgt
      841 tccaaaatcg cggaacgggt gattcaaccg gcggtaaaat cccttgttgc agttgggctg
      901 cctgtattaa ggaccttggt gaccaaccgg ggttcctatc aatatgtgga ggttcggacg
      961 atgaccgcca ctgcctatta tcccggaccc gagagctgcg ggaagtacgc ggtgaatggt
     1021 gtaacctaca ccggtaaaaa ggcgggtttc gggatcgtcg ctgttgaccc gcgggttatt
     1081 ccgctcggta ccaaggtata tgtcgaaggt tacggcaagg ctgaggctgc cgatattggc
     1141 ggcgcgatca agggcaacaa gatcgattta tgttttgaaa cctttcgtga ggctgcatta
     1201 tatggggtta agaagatcaa ggtttatatc cttgagtgag gtgacacggg tctctaagcc
     1261 tactggctaa ggttccgggt taggactaca ggcgggcgtt gcccgctttt tttatggccg
     1321 ggataccaag ggaggaatgt actcttgttt aacgttgaca agtatcagga tcagtttacc
     1381 tattatgaac tgcaggactt ggccgccgga tcccgggtaa agttggttcc ggaacggggc
     1441 ggtatagttg ttgggtttag tctgggggat caagagcttt tatacttgga taaggaaacc
     1501 ctcaataatc cggagcagaa tgttcgcggt ggcattccgt atctctttcc catctgtggg
     1561 cggttgtccg atgacgaata cacctggaat ggagagaaat accggatgaa tcagcacggt
     1621 ctggccagaa atcttccctg gactgtcctg aatcaggaca cccgggagga ggcagccatc
     1681 accctgcaat tgaagggcga cagggcgacc cgttcccaat accccttcga ttttgaactg
     1741 gatttcaaat actccctgaa ggggaagacg ctccggattg atcaggaata ccggaactgt
     1801 tccggtgtcg aacttccgtt ttacgccggg tttcatccgt atattcgggt tgcggagaag
     1861 gaacgcctga tatatgatat ttccgccagt gcctatttgg attttgagga cctccggatt
     1921 aagccgtata ctggaaacct ggatctaacc catgaaaatc aagcaaaact gtttatggat
     1981 catgccccgg gccgcttgtc attccgggat cctgttttga accggaaatt tcattatgaa
     2041 tacggcccgg agtttaaata cccggtgctg tggacgattc cgggtcagga tttcgtttgt
     2101 gtcgagcttt tcatggcaag gcccgacgcg ctcaataccc gggaggatct gatattcatt
     2161 ccccctcacc ggacgcatca gaccttcgtg gcgattacgg cggaactcac caagtaacgg
     2221 tcaggaacgg gttttcctga ctttgatgtt gtcgcgggcc actccggggt cgcaagggga
     2281 tttacgaaaa agggtaatct ttccggaaaa gttgacgata aatataaaga aaatgcttgc
     2341 cgtggtgata cgggatggcg atccggaagg gttttatgcc gcttgctcgg taaaaactgt
     2401 ggagaagcct agtataccaa gcaatgcact gggaaaaagg gggtagcgta atgtttgagg
     2461 aacgcagaaa tctaacgagg gtgaacactc atattgtcgc ggagattaat gttcagggaa
     2521 atccggaacg gctttgcggt tatatcgaaa atatcagcga aaagggaatt ggagtactct
     2581 cactggatcc atttatcaag ggtgcggagg ctgccaccac ttttcaattg ccggggattc
     2641 cccggagttt agaggccaaa gtcagtctct taaactccaa gagcggcctc tataatcttt
     2701 actaccacag ttttcgtttt gagggtttat ctgagagcga gcaaatggcc attagccact
     2761 atatcaagca gcaggaatcg gtggaaaacc ccgcatgaaa acggggcggg tatggtaagg
     2821 gcgggttata aggacgggtt ataaggacgg gttttgaacc cgcccctaca acccgccctt
     2881 aatttttttc attcaaaacg tcgcaatgcc acgcaggcgt tggtggcgcc aaaaccaaag
     2941 gagttggaga ccgctacctt taagtccgcc ttttgggcat gattcggcag ataattcagg
     3001 tcgcaatcgg gatcgggatg atcgagattg atggtccccg gggctgtttg atggtaaaga
     3061 gtgagcgccg tataagccgc ctcaatggct ccggccgcgc ccaacaggtg accggtcatg
     3121 gatttggtag aactgatcaa gagttttgaa gcgtgttcac cgaaacagtg tttgatggcc
     3181 ttggtctcca tctggtcgtt gatgggggtt gaagtaccat gggcatttat ataatctact
     3241 tcagcggggg tgatccctgc agactgaagc gtcagctcca tgcatcggga ggcacccatc
     3301 ccgtccggtg ccggcgaagt gatatggtaa gcgtcgctgt tcattccaaa gccgatgacc
     3361 tcggcataaa tcttggcccc gtgatgaagc gcatgttcca actcctcaag gatcaaaatc
     3421 cctgcacctt cacccatgat aaaaccgtca cggtcagcgt caaaggggcg gctggcttta
     3481 tcgggagcat cgtttcgggt tgacagggcc ttcatattgc cgaatcccgc catcgccaag
     3541 ggaatgatag ccgcttcggc accaccggtg atgaccgcat cggcatcccc acgacgaatg
     3601 atatgaaagg attcaccaac ggcatgggtc ccggtggcgc aggcggaaac cggtgaaaag
     3661 ttgggtccgg tagctccggt acggattgag atttggccgg gggccagatt gatgacacag
     3721 ctgggaatga aaaaaggcga gatccggtcg ggtccttggg ttacgaaggt atcctggtgc
     3781 tggcagacag tttcaatccc gcccatcccg gatccgataa tcactccgac cttcgatgcg
     3841 ttcgccgggg tgatggtcag gcccgagtcg gccaatgctt cattggtggc ggcgactgta
     3901 aactgaatga accggtccat cttgcgaatc tcttttttct ctaaatattg accgggatcg
     3961 aaccctttga cctcggctgc gatttttgtc ccgaattttg aggtgtcaaa tcgagtgata
     4021 gatccaacac cggaacgtcc cgtaacgagc gcttcccaat tcgcttcggt agtcaatccg
     4081 agtggagtga caaggccgat tccggtaact acaacgcgac gattcatacc aattcacctg
     4141 ctttaaggtt atttgtgaat gcgcatagag aaataaagat ctaaaccaaa tgtgaattac
     4201 aatgaaaacc ctgaataatt cgaataaaat aaggctcaac ttgagaggag ctgtttgccg
     4261 atgacagttg aaaccttccg gaagactttg atttcgtcgg gagagagcgc tccctgaagt
     4321 ttatggtcca tttcgttaac cagggattta atcgtttccc tggcgatgat cccgttcgga
     4381 gtcaaaaaaa tgagtagtcc cctgcgatcc cgcggatcac ggcgccgttc aatgagccct
     4441 tttttttcta aacggtttaa caacccggtc atactggaac tctccagatc cagtttggtg
     4501 ccgagggatt tggggctttc accgcccccg ggagatattt ccagtaacaa taaggctttg
     4561 ggcacggtca gatcaaaacc ggcaagccgt tgattgtaat agcgggaaag cttctgccac
     4621 gtattcttga ttaacaggca gaacaaggga cgattggaat tttcctgatg gatatgttcg
     4681 ccattggcca ttttatcaca gactttgcaa taaaattgaa attcgaaaat agttcgtata
     4741 cgagttattg gatacaaaaa atatagcatg gattcccaat gaagtcaagc ttgtactatc
     4801 cacagttacc atattgagct gcatgttggt ccggagcgtt tagggatttt tcagcgcctc
     4861 ggggggaaga ttaaatcgtg gagataaaat gaaactactc gacttaaaga aaataatccc
     4921 tgtcccccca aaattttacg aggttgattt caacgggttg aacaacgact ccaagaagat
     4981 tcagccgggg gaactcttta ttgccgtccg gggttacaaa accgacggtt atatctattt
     5041 ggatgaagcc atccgccgtg gagccagcgc ggctattgtc gaggccgatc aaaccgggcc
     5101 tgccggattt ccggtgctcc cggtagccta tacccggaga attcagggac aagtggcggc
     5161 acaattttat ggcgagccct cccgtaggat gcgcttgatc ggagttaccg gtaccaacgg
     5221 caagactacg gtaacccact tgattgagca tctgttgaat tcaagctata tccccaccgg
     5281 attgatcggt acggtttgga tcaataacgg ccgggaacgg cagtattcaa cccggactac
     5341 cccggattcc atcgaactcc agcgtttgtt cgccggaatg atggcaaacg gtatcgaaac
     5401 ggtggtgatg gaagtctcat ctcacgcctt agcgctggag cggaccgcgg gatcggagta
     5461 tgatgtggct gttctgacca acatcactca tgatcatttt gattttcacc gaaactacca
     5521 gcaatatatg gaggcaaaac ttcaactctt ccgcaacctt gatcccgccg gcaagcccgg
     5581 gaaatatgct gtgctgaacc gggatgatcc gagttcggaa cagatcgcct cggaatgccg
     5641 ggtcccggtc attttttacg gggagggcga cgggtctttt agccggctgg aagctgtgga
     5701 acgcacgggt ttagagagcc tcatccgaat ccggctgggg gatgaggaat gccgggtccg
     5761 tacccggctg ccgggagagt ttaacattta taatattctg gccgctgtga ctgtagccta
     5821 ccgtgagggt ctttcggttg aggatattga agcggctttg cctgattttc cgggagttcc
     5881 cggcagatac caagagatta attgcggaca accgttccgg gtaatggttg atttcgcgca
     5941 taatccggcg gcgcttgaga atatactcaa aatggcaggg gaatataccc ggggtcgtcg
     6001 gatcctggtc tttggttgcg aaggggagaa ggaccgtgtc aagcggccgt tgatgggggc
     6061 gattgccgcg tatcatgcgg aagtgccgat tttaacctcg gacaatgtct atcatgaaga
     6121 tatcctccag atcttcaccg atgtccttca aagcctctct ccgggggaac aaggaagatt
     6181 catcatcgaa ccggaccggc gtaaagccat aaagaccgcg gtaagcatgg caaaccctgg
     6241 tgattttctg attgtcgcgg gaaaaggtca tgagcagtat ttggtccaag ggaaccagaa
     6301 actccatttt gatgatgctg aagagttgcg ccggttgctt cagagaactg aatagatgaa
     6361 gacggtgtta cgccgctgcg ataacggtaa ccggttatag agcctgaatc gtcacccaac
     6421 gtagtttgat ctttcgagtg aaccggacgt ttgacaaaaa agcaaccccc ggaataccat
     6481 ccggaggttg cgactgttta taccatgtag ctatttaagg tatgtcaaag cttccctggg
     6541 tatgtacggc acgccgttga cgattaagga gatattcctc aaggaatggg gtttaccatt
     6601 tacataggtg gtattggtat tggcaatcaa ttcaacgacg gtgttgccgc ttgtcaggat
     6661 aatataagga cggttgacac cgtacttggt atccagggta cggactcccc attcatagcc
     6721 ggtgtctccg gccattacaa aaagcttttg gttgacgtcg gccaggctga cccccatatc
     6781 tttggcaatg attttagcga tatcagtatt ctctatcaag ccggcaggtt ttccaggccc
     6841 gtaggcgtat agaaaaactt cttccccggt atggccgatg gtggtccagc cgatgccgga
     6901 acgcttgctg attatcgggc ccactgtata attcatcgaa ccagccttgg tttttttgat
     6961 ggcatcgatc tcttccgtgg ttaagtcgga gattccgaaa gattgattca tcactgcggt
     7021 aatattactt ttgtccgcgt taaagacttt ttcgaggcct t
//
LOCUS       DASVMB010000470         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_280779,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000470 DASVMB010000000
VERSION     DASVMB010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280779"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..924
                     /locus_tag="VF531_13185"
     CDS             64..924
                     /locus_tag="VF531_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7714961.1"
                     /translation="MLNFMIALFTLLCILPFVLVTILSFTDEETLLRNGYSYFPAKLS
                     LKAYEYVFKSSDQLISSLAVSVTVTVIGTFLGVLIMSMYAYALYRKDFRYRRFFAFLA
                     FFTLLFNGGLVPTYIVCTQMLHLKNSIFALILPLGVDAFYIMVLRTFFQTALSESVID
                     SARIDGAGEFKILSAIVFPMSLPGIATIALFTTLAYWNDWFQALLYIQQSNLYPLQYL
                     LVAIQNKMDFMMQNRAILSAHEVEIYKTMPAEGSRMALVFLSTLPITLAYPFFQRYFI
                     QGLQVGAIKE"
     gene            1046..>2421
                     /locus_tag="VF531_13190"
     CDS             1046..>2421
                     /locus_tag="VF531_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7714962.1"
                     /translation="MGRVLKLKKVIPVVVVTTLLCVLLLGVCGVQAAGKPYEIVWYTI
                     GTPQKDIPVVMAELSKYTLKKINATVKMFQYDWGEYNDKMRVIISSGEPYDICFTCSW
                     SNNYDANVTRGAFLPLNDLLQKYGKGITKAVHPLFLEANRIGGKLYAIPVNKELTPQS
                     RWVFNKRLVDKYHIDITKATTLESLEPMLKTVKDGEQTLDDVLVVRNDYNLPRCTADY
                     EFIGPQELGLAVPMGSKDCQVVNYYHEPSFKKGLATVRGYYLAGYLKKDASTLTSSDE
                     VYKTGNWFVREAGWLPGADQIWTRSEKDPVVSTPIAGKFYISANQLSGSMMAISVTSK
                     NPGKAMQFLNLLNTDRYVRNLVDSGIENVHYKMVNGRQVDLERGTGDYNMPSFSLGNR
                     FICNLYTDDPADLWQQYEKLNASGTKSPIIGFKPNLEGIKKEIAAIQNIHAEYYRPLV
                     TGTVDPDE"
BASE COUNT          627 a          553 c          594 g          647 t
ORIGIN      
        1 ccgggtaata aagcccggca taaggcaagg cccggtacaa ggatatccag agccgggaat
       61 atcattctta atttcatgat cgctttattc acattgctct gcatactccc ctttgtactg
      121 gtgacaattc tctcttttac ggatgaagaa accttgctca gaaatgggta tagttatttc
      181 cccgctaaat taagcctcaa ggcgtatgaa tatgtcttca aaagcagcga tcaactgatc
      241 agctccttgg cggtcagcgt cacggtcacg gtgatcggta cttttttggg cgtattaatc
      301 atgtcgatgt atgcttacgc gctttaccgc aaggatttcc gctaccggag attcttcgcc
      361 tttctggcct tttttacatt gctgtttaac ggcgggctgg tgccgactta tatcgtctgt
      421 acccagatgc tccatttgaa gaacagcatt tttgccctga tactgcccct tggggtggac
      481 gccttttaca tcatggtatt gcggaccttt ttccaaacgg cgctttccga atcggtcatt
      541 gattcggccc gtatcgacgg ggccggagaa tttaagatcc tgagcgccat tgtctttcca
      601 atgtcccttc cgggcattgc caccattgcc ttgtttacta ctttggccta ttggaacgat
      661 tggtttcagg cgctgttata cattcaacaa tccaatctgt atccgctcca atatttgctg
      721 gtagccatcc agaacaagat ggatttcatg atgcagaatc gcgcgatact ttccgcccat
      781 gaagtcgaaa tctataaaac aatgcctgcc gaaggctcca ggatggcatt ggtgtttctt
      841 agcaccctac cgattacgtt agcctatccg tttttccagc gttatttcat tcaagggtta
      901 caggtgggag ccattaagga ataggattta tccattcagg caagtgccgt ttgaaccgta
      961 agctgttgca cggaactttg attcggcata aacgccttac ggcgaaggcg gttttttcaa
     1021 tcaaaataaa aaaggagggt tttttgtggg aagagtgttg aagttgaaaa aggtaatacc
     1081 ggtagtggtt gtaaccacgt tgctatgtgt attgctgctg ggggtctgcg gagtacaggc
     1141 ggccggcaag ccttatgaga tcgtctggta cactatcgga acgcctcaga aggatatccc
     1201 ggtagtcatg gcggaattga gcaaatacac attgaagaag attaatgcca cggtgaaaat
     1261 gtttcaatac gactggggag aatacaacga taaaatgaga gtcatcatct cctccggcga
     1321 accctatgat atctgtttca cgtgctcatg gtcgaacaat tatgatgcca atgtgacccg
     1381 cggggcattt ttgccattaa atgatctgtt gcaaaaatac gggaaaggca ttacgaaagc
     1441 cgtacatccg ttgtttcttg aagcgaatcg aatcggaggc aaactgtacg ccattcccgt
     1501 caacaaggaa ttgactccgc aaagccggtg ggtatttaac aagcgtttgg tggataaata
     1561 ccacattgac atcaccaagg cgactacgct ggaatctttg gagccgatgt taaaaacggt
     1621 caaagacgga gagcagactt tagatgatgt cttggtggtc cggaacgatt acaatttacc
     1681 aaggtgcact gctgattatg aatttatcgg gccccaggag ctgggtttgg cggttccgat
     1741 gggttccaaa gattgccagg tcgttaatta ttatcatgaa ccctctttca aaaaggggtt
     1801 agcgacggtt cgcggttact acctcgccgg ttatctgaaa aaagacgctt ccaccttgac
     1861 ttcttcggat gaagtttata agaccggcaa ttggtttgta cgggaagccg gctggctgcc
     1921 gggagccgac cagatctgga ccagatcgga aaaagacccg gtagtttcaa ccccgatcgc
     1981 aggtaagttc tatatttccg ccaatcaact cagcggttcc atgatggcga tctccgtcac
     2041 ttccaaaaac cccgggaagg ccatgcaatt cttgaatctg ttgaataccg acagatatgt
     2101 cagaaacctg gtagactcgg ggatagaaaa tgttcattac aagatggtca acggcaggca
     2161 ggtggatctg gagagaggca ccggcgatta caatatgccg tccttctccc ttggcaaccg
     2221 gttcatctgc aatttataca ccgatgatcc ggcggatttg tggcaacagt acgaaaagct
     2281 aaatgcttcc ggaacaaaat caccgataat cggctttaaa cccaatctgg aggggataaa
     2341 aaaagagatc gccgccattc agaatattca tgccgagtat taccggccgc tggtgaccgg
     2401 tacggttgat cccgatgagt a
//
LOCUS       DASVMB010000471         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_56412,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000471 DASVMB010000000
VERSION     DASVMB010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56412"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..776)
                     /locus_tag="VF531_13195"
     CDS             complement(123..776)
                     /locus_tag="VF531_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7714963.1"
                     /translation="MADKSFQNYTERHIVWYGHSAFKITAESGAVLYIDPFRLPSGTG
                     EADVILISHEHYDHYDPQLIKGLRKSRSTVIVPRGMAYDGLQGMKLGETVQAGPFRVT
                     GIPAYNINKNFHPQVQYWLGYLIEVDGVRIFHAGDSDNIPEWKGMKPDIALLPVGGTY
                     TMDVAEAAKAVAALGAELAIPMHYGSIVGVKEDGVHFAALLKPGQGLVLTPHCPVKN"
     gene            972..1538
                     /locus_tag="VF531_13200"
     CDS             972..1538
                     /locus_tag="VF531_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714964.1"
                     /translation="MEQIALILKAYRWLDQITPLQTDCGTLCDKACCLSTEDVATENF
                     TQNSGMYLFPGEVSIQSAAAYVSLHPVNIATTHGAYRSVLAVCNGSCPRHLRPLACRI
                     FPLTPYVDPDGEWLLRMDLRGRSLCPLVRQMQPDELEPDFIAAVWTVINLLATDPEIR
                     YFMTLLSRIQDETAALFTRFGIQSSTQG"
     gene            2098..2520
                     /locus_tag="VF531_13205"
     CDS             2098..2520
                     /locus_tag="VF531_13205"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEX7714965.1"
                     /translation="MESIRCEDFKKRLVELTIKSGLSGFPVKRRDQLILLRAVSNGFQ
                     PDIIYEEKTVNEIIRTQVKCLKCLTGLDHVTVRRQLVDERFLSRENDGSRYRLGPGPD
                     SVQFETTVTDLNIEALLASAEAELAARKKAFMERQTTL"
     gene            complement(2692..4059)
                     /gene="lpdA"
                     /locus_tag="VF531_13210"
     CDS             complement(2692..4059)
                     /gene="lpdA"
                     /locus_tag="VF531_13210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7714966.1"
                     /translation="MHDLIVIGGGPAGYRAAIRAGRGGLSVLLAEKRFIGGVCLNEGC
                     IPSKTLLYSAKLYEEARNSSKYGVTTGQVTYDQHTVLERKTKVIRTLAAGINRQLREA
                     GVTVASGAAEIEGKSNSGYSVRVGDEVYEAKNLLLATGSEPIIPAIPGVATGLKSGLV
                     LTSRELLDLAQLPESLVIIGAGVIGLEMAAYFNAVGTHVTIIEMLDHIAGNADPEIAA
                     MLLKEYRKQGIDFRLGSKVTALDDGPVRFITDGREEVVSADKVLLSIGRKPAVTDLGL
                     ERIGLTLERGAITTDPSCRTELPGVYAAGDVNGRSMLAHTAYREAEVCIDRLLGKEIQ
                     MDYGAIPAVIYTRPEAAMVGETEETAAIKGIDYEVAKLSMRYSGRFLAENEGGDGVCK
                     LLIEKHNRKLIGAHLIGSYASEIIYGAAMLIESGRPIQSISEVVFPHPTVSEILRDGL
                     LEAVK"
     gene            4255..>4548
                     /locus_tag="VF531_13215"
     CDS             4255..>4548
                     /locus_tag="VF531_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714967.1"
                     /translation="MVSKKFILLSVVSLISVLIAGCDGLGGLGSLGAPGGSGGSGGRT
                     DDNQPINLLRNFSAELGTGNTPAEWWNNPATDKIEWASEGKTGTHSLRISYLSN"
BASE COUNT         1171 a         1171 c         1018 g         1188 t
ORIGIN      
        1 tgtaattcat gatatgtgta tctactaccg gatatgagat ttcgatatcg aacgaggtaa
       61 cagatttgtt atttccccga gtcatccaca gtggaaatga ttcctgcgga tcttcttata
      121 ttctaattct tcacggggca atgtggagtc aataccaacc cctgccccgg tttaagcagt
      181 gccgcgaaat ggacgccatc ctccttgact ccaacgattg aaccataatg catcgggatc
      241 gccagttccg ctcccaatgc agctactgct ttagcggctt cagcgacatc catggtatag
      301 gtacctccaa ccggtaacag cgcgatatcc ggtttcattc ccttccattc gggaatgttg
      361 tcgctatcgc cggcgtgaaa gatccggacc ccgtccacct ctatcaggta ccccagccag
      421 tactgcacct gaggatgaaa gttcttattg atattatacg ccggaattcc ggtcacccga
      481 aacggccctg cctggaccgt ctctccgagt ttcatccctt gcaagccatc atacgccatg
      541 ccccggggaa cgatcaccgt ggatcgggat tttcgtaaac ccttgattaa ttggggatca
      601 tagtggtcgt aatgttcatg gctgataagg atgacatccg cttccccggt tccggaggga
      661 agccggaagg gatcgatata gaggaccgct cccgattccg ccgtaatctt gaatgccgaa
      721 tggccatacc aaacgatatg ccgttccgtg tagttttgaa atgatttatc agccataacc
      781 gaagcgattc ctcccgcaat aaatataggt cgacattgtg gtggagactc aagtttataa
      841 tgcctttttc ttcaaaaatc gcagtctctc tgatatattt tctacaactc gcatgaactc
      901 attccttttt cccggatttg tatcttttct tgccatttgt ataatactgc cacttccggt
      961 agaatggaaa catggaacaa atcgcgctca ttctcaaagc ctaccggtgg ctggaccaaa
     1021 tcactccgct gcaaacagac tgcggtacgt tatgtgacaa agcttgttgc ctgagtacgg
     1081 aagatgtggc taccgaaaac ttcacccaaa attccggaat gtatctgttt cccggtgagg
     1141 tttccattca atccgctgct gcttacgtat ccttgcatcc tgtcaatatc gctacaacac
     1201 acggtgctta ccgatccgta ttagccgtat gtaacggcag ttgcccgcga catctccgtc
     1261 cgctggcctg ccgaatcttc cccctgactc cttatgtgga tccggatgga gagtggctgc
     1321 tgcgtatgga tctccggggc cggagtcttt gcccgctggt gcgacagatg caaccggatg
     1381 agctggaacc ggattttatc gcagcagttt ggacagtaat caacttattg gcaacggatc
     1441 ccgagattcg ctactttatg accttgctct cccggattca agacgaaacc gccgcgctat
     1501 tcacgcgttt tggaattcaa agctccaccc agggttgatt aacgccagcc ttttcttcag
     1561 ctcaattgtt atccatgttg cgcggctcct ttttctcgct gtaccctgaa tggaactccg
     1621 acggtaagtc tggttcaatg gaacagcaaa ctatacttat attacatcgg aatgctaaca
     1681 ggagttaagc atagattgat gataaatttt ttcgttaaac aacaaataca ccgtactttg
     1741 aaataaaggc catgacagtt catggccttt ttacccaatt tcacctcagc tattgatagg
     1801 actgactcga gaggacgtct accaacaaat aatggcgtct ttcgtttgac tgctaactgc
     1861 agtaagtttt ttcccgttct ccaggtcgat gactgtgatt ttcgaaccat tgggaaaata
     1921 ttgctcattc aaaacgcaca ttgacaatct ataaacatat caatataatg tttatagatt
     1981 atccaacatg tgagtgattt gtttactaaa ataggcaatg taaaccgaaa tgtctacact
     2041 gacctgtaaa gacaatttac cttgattggc atagtcatta atgggagtgt gggaataatg
     2101 gaatccattc gttgtgagga ttttaaaaaa cgccttgtcg aactgacaat taaaagcggc
     2161 ctatccggat ttccggtcaa gcgccgcgat caattgatcc tattaagggc tgtcagtaac
     2221 ggttttcaac cggacatcat ttatgaagaa aaaaccgtga atgaaatcat ccgtacccaa
     2281 gtcaagtgtt taaaatgctt gactgggctt gatcatgtca cggttcgtcg ccaattagtg
     2341 gatgaacggt ttttatcccg ggaaaacgac ggctctcgtt atcgtctggg ccccggccct
     2401 gattcggttc agtttgaaac tactgtaaca gacctaaata ttgaagcgct attggcttca
     2461 gccgaagcag aactggcagc cagaaaaaag gctttcatgg aacgccagac tactttatga
     2521 cttcaataaa gaataatccg gggaatatga ttattgcgcc cgccgctaga gagagcaaga
     2581 gtgagtcgta aacaacgaag acgactcacc cccgcccctc tctcccggcc agcgggaaag
     2641 aggggagtag gcaaattcaa cgcagtaaaa aaaactaccg tatactatac tttacttcac
     2701 cgcttccagc agtccgtccc ggagaatctc ggataccgtg ggatgaggaa agactacttc
     2761 actgatggat tggatcggtc tgccgctttc gatcagcatt gcggcaccgt aaataatctc
     2821 cgaggcgtag ctcccgatca gatgtgcacc gataagctta cggttatgtt tctcgattaa
     2881 cagcttgcaa accccgtcgc cgccttcgtt ctccgctaaa aaccgcccac tgtaacgcat
     2941 tgaaagcttg gcaacttcgt aatctattcc tttgatagcg gccgtttcct cggtttcgcc
     3001 aaccatagcc gcctcgggac gggtatagat cacagccggg atggccccgt aatccatctg
     3061 aatttcctta cccagtagtc ggtcgataca aacctccgcc tcccggtagg cggtatgagc
     3121 cagcattgaa cggccgttga catccccagc cgcgtatact ccgggtaatt cggtccggca
     3181 tgaaggatcc gtagtgattg cgccccgttc taaagtgaga ccgattcgtt ccaaccccag
     3241 atcggtcaca gccggtttcc tgccgatact aagcagcacc ttatcagcgg aaacaacctc
     3301 ttcccggcca tcggttataa agcggaccgg cccgtcgtcc aaggcagtga ccttggaacc
     3361 aagccggaaa tcaatcccct gtttccgata ttccttcaaa agcattgcgg caatctccgg
     3421 atcggcgtta cccgcgatat gatcaagcat ctcgatgatg gtaacatggg tgccgacagc
     3481 attaaaatag gccgccatct ccagaccaat cactccggcg ccgataatca ccagcgactc
     3541 cgggagttga gccaaatcca acagctcgcg gctggtaaga accaaaccgc ttttaagccc
     3601 ggtagccact ccgggtatag caggaataat cggttccgac cccgtagcga gcagcagatt
     3661 cttggcttca tacacttcgt cccctactcg aacgctgtaa ccggaattgc tcttcccttc
     3721 gatctctgca gcgccgctag ccacggttac cccggcctca cggagctggc gattaattcc
     3781 ggcagccaaa gtccggataa ctttggtttt ccgttccagg accgtatgtt gatcataggt
     3841 tacttggccc gttgttacac cgtatttgga actgtttcgg gcttcttcgt aaagcttagc
     3901 cgaatataac aaggttttgg agggaatgca cccttcgttt aaacaaaccc caccgatgaa
     3961 ccttttttcc gccaataaga cgctgagtcc gccacgtccg gcccggatcg cagcccggta
     4021 tcccgccggc ccgccgccga tcacgatcag gtcatgcata tagggcacct ccatttagtt
     4081 gaaaaatgca tgaaataaaa actactgtag gattttccag aaatttatcg aaagaaatat
     4141 tgaattccag tttgttcctt gatggtgatt gaaccaacat attctgcaat tgattaaaaa
     4201 aagtaatatt tctaatatca gcgaaaaact acatccagaa gaaggataat tcaaatggta
     4261 tcaaagaaat tcatcctact ttctgtagtg agtttgataa gcgttttaat tgcaggttgt
     4321 gatggccttg gcggccttgg cagtcttggt gcccctggtg gctctggtgg ttctggtggc
     4381 cgaacggacg ataatcaacc tattaattta ttaagaaact tctcggcaga gttgggtacc
     4441 ggtaatacac cggctgaatg gtggaataat cccgccactg acaaaattga atgggcctca
     4501 gagggaaaaa ccgggactca ctcattaaga atctcttatc tctcaaat
//
LOCUS       DASVMB010000472         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_719881,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000472 DASVMB010000000
VERSION     DASVMB010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719881"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /gene="rbfA"
                     /locus_tag="VF531_13220"
     CDS             <1..175
                     /gene="rbfA"
                     /locus_tag="VF531_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7714968.1"
                     /translation="ALEGLESAKGFIRTELGHRIRLRHTPEIHILEDNSIEHGMRITE
                     LLKEVQKNNGGEG"
     gene            159..1154
                     /locus_tag="VF531_13225"
     CDS             159..1154
                     /locus_tag="VF531_13225"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX7714969.1"
                     /translation="MVEKVNLLAQFQEFMNRIRPKQRFLLSCHVHPDGDGIGSLLALG
                     LALESIGKDVRMVSSDGVPGVYNYLECSPAIIKDLAGDFQPEILICLDCSEKERVALP
                     AAVWDSPGLIVINIDHHLTNLGFGDLNLIEPNAAATGEIILHLLQAVEIPLNRAIAMA
                     LYTAIATDTGFFRFANTTADTLEACTYLVKNYRIEPSKIAEQVHEQKSYNSIRLLGEV
                     LSTIRLEHGGKTAWAILDQQMLTKYSVENEETESYVNYARSIEGVEIGILFKELKPNE
                     IKLSWRSTAAVDVSKLAAYFGGGGHARAAGCSLNGPLPEIIREVLNFVGNYYGAN"
     gene            1154..2071
                     /gene="truB"
                     /locus_tag="VF531_13230"
     CDS             1154..2071
                     /gene="truB"
                     /locus_tag="VF531_13230"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7714970.1"
                     /translation="MWHGILVVNKERGMSSHQVVAKLRRILGQSEIGHSGTLDPEATG
                     VLVVGLGQATRSFQYMDEAVKIYRAELTLGQTTDTQDATGRIIDTQPDMRIHRDQLEQ
                     AIGRLTGEIHQVPPMFSAVKLHGQKLYDLARQGLEVEREARPIKVYHWQIVDPQSEYT
                     YATQLTCEITCSKGTYIRTLIHDLGQTLGCGAHMSALIRLRSGPFSLEDAVSLSGVAE
                     YFAAGRIEELLFSINTALSHFIPIWPQNEDLPKLVHGGKLSFDRYPASQNIGTLARAL
                     DNDSRVLAVLRLEDGGTHRYWQPVKVFHY"
BASE COUNT          535 a          547 c          590 g          519 t
ORIGIN      
        1 agccctggaa ggtttggaaa gcgccaaggg ttttatccgc accgaacttg ggcatcgaat
       61 ccgtttgcgt cataccccgg aaattcatat tttagaggat aactccatcg aacacggcat
      121 gagaattacg gagttattga aagaggtcca gaagaacaat ggtggagaag gttaatttgc
      181 tcgcccaatt ccaggaattc atgaaccgta tccggccgaa gcaacgtttt ttactcagtt
      241 gccacgtgca tcccgatggg gatggaattg ggtcattgct cgctctgggc ctggctctgg
      301 agagcatcgg gaaagatgtc cgcatggttt cgagcgatgg ggtgccgggg gtctataact
      361 atctggagtg cagtccggca attatcaagg atctggccgg ggattttcaa cctgaaatct
      421 tgatctgctt ggattgttcg gagaaagagc gggtagccct tcccgccgca gtctgggata
      481 gccccggatt aatcgtgatt aacattgatc atcaccttac gaacctgggc ttcggtgacc
      541 tgaacctaat tgaaccgaat gccgcggcaa caggggagat catcctccat ctgttgcagg
      601 cggtggagat tccgttaaac cgggcgattg ccatggcatt atataccgcc atcgccacgg
      661 ataccggatt tttcagattt gccaatacca cggcggatac cctggaagcc tgcacctacc
      721 ttgtaaagaa ttatcggatt gaaccttcca aaatcgccga acaagttcat gagcagaaga
      781 gttacaattc catccggctt ttgggtgaag tcttaagtac cattcgcttg gagcatggcg
      841 gtaaaaccgc ttgggcaata cttgaccagc agatgctgac caaatactcg gtagaaaacg
      901 aagaaaccga aagttacgtc aattatgccc gttccatcga aggggtcgag atcgggattc
      961 ttttcaaaga acttaaaccg aacgagatca agctgagctg gcgttccacc gctgcagtag
     1021 atgtcagcaa gttggcggca tatttcggag gcggggggca cgcccgtgcc gccggatgca
     1081 gcttaaacgg tccgcttccc gagatcatcc gggaagtatt gaatttcgtc ggtaattact
     1141 acggagcgaa ttgatgtggc acggaattct ggttgtcaat aaggaacggg ggatgtcgtc
     1201 ccatcaagtc gttgccaaac tccggcgtat tcttggccag tccgagatcg gacattccgg
     1261 gactttggac ccggaggcca ccggggttct ggtagtgggg ttgggacaag ccacccgttc
     1321 ttttcagtat atggatgaag ccgttaaaat ctatcgggca gagcttaccc tgggtcaaac
     1381 gacggatacc caggatgcca ccggtcggat cattgacacc caacccgaca tgcggattca
     1441 ccgcgaccaa ttggagcagg caataggccg gcttaccgga gaaatccacc aggtcccgcc
     1501 gatgttctct gcagtcaagc tgcacggcca gaagctctat gacttggcgc gtcaggggct
     1561 ggaggtagaa cgggaagccc ggccgattaa ggtatatcat tggcagattg tggatccgca
     1621 atccgagtat acttacgcga cccagttgac ctgcgagatt acatgttcca aaggaaccta
     1681 catccggaca ctcattcatg acctgggcca aactttggga tgcggcgccc atatgagtgc
     1741 cttgatcagg ctgcgatccg gaccattttc cctggaagac gcggtttcgc tttcaggggt
     1801 agccgaatac tttgcagccg gccgaatcga agagctgctt ttctcgatca acaccgcttt
     1861 gagccatttc atcccaatct ggccacagaa tgaggacctt cccaaattgg ttcatggtgg
     1921 taaactctcg tttgatagat atcctgcttc ccagaatatc ggcacgctcg cccgggcttt
     1981 agacaacgat tcccgggtac tggcagtgtt acggctcgaa gacggcggaa ctcaccgtta
     2041 ttggcaaccg gtgaaggttt ttcattatta gcagtccatt ttgcggatcc catttacata
     2101 gcgtcggtac tttaattgtt ggttaaggga gcataactca accaatgcac atctggaact
     2161 cactcgatgc cgcacgtcag gaactgattc a
//
LOCUS       DASVMB010000473         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_498662,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000473 DASVMB010000000
VERSION     DASVMB010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498662"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VF531_13235"
     CDS             complement(<1..1845)
                     /locus_tag="VF531_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7714971.1"
                     /translation="MAKKYVIIGGVAGGASTAARLRRLDEQAELILIEKGEAISYANC
                     GLPYYIGGVISERDQLFVQTPAGMKKRFNIDVRVKHEVLEIDRPKKELVVKDLQQGRT
                     YRESYDKLVLAPGSYPIVPKIPGTQLQQVFTLRNIPDTDRIKQYLTEHKVNSAVVVGG
                     GFIGLEMAENLNQAKKQVTLVELADQLLNNLDFEMAAPVHQELRSHGIELILGDGLAA
                     ISEAGKGLDVILKSGRKITADMVIMAVGVRPETKLAAEAGLKLGATGAIHVDESLRTS
                     DPDIYAVGDAVEIREFISGKESWVPLAGPANHQGRLLADILTGRKEIYHGAQGTAIVK
                     LFDLTAGSTGLSEKALKKWGIPYRSTITHSNSHATYYPGSVPLTLKLLYAPDNGRILG
                     AQVVGYDGVDKRLDVLATAIRFEKTVWDLTELELAYAPPFSSAKDPVNIAGYTATNLL
                     NQDMDVITWDQLLAESGNQNYLLDVREPVEFQLGALPGAVNIPLPELRGRLAELPKDR
                     PIIVYCQVGLRAYIALRILRQHGFENVRNLSGGYKTFHAVIQERQQPVSGEASHSVVA
                     TVAYASNGSEKEEPSMVMDIIKVNACGLQCPGPILQLYTRMQQLNDGEM"
     gene            complement(1913..2206)
                     /locus_tag="VF531_13240"
     CDS             complement(1913..2206)
                     /locus_tag="VF531_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7714972.1"
                     /translation="MQRPDDLELLTAKAELLKSIAHPVRLCIVKGLIEQGECNVNKIQ
                     DCLALPQSTVSQHLGKLRDLGIVRSRRQGVEVYYQVVNEDIQRIVRSLFPTSS"
     gene            2544..2732
                     /locus_tag="VF531_13245"
     CDS             2544..2732
                     /locus_tag="VF531_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714973.1"
                     /translation="MEHRGRPEAVAPAGMTRICVLAPRGTAVAIAPMAMPMGTPCGTM
                     PMGMMNPMMHMDPMDRMC"
     gene            3077..>3605
                     /locus_tag="VF531_13250"
     CDS             3077..>3605
                     /locus_tag="VF531_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HEX7714974.1"
                     /translation="MYINKWLAITALGLLLLARTAVADNSMALSFAGLPAGPEVQAIN
                     RDGAVYINLPFLNKYLNIVTAWDISASDLFFKFGKISVKMYAKNRSYYLDGRSQPSLN
                     QAPFEQDGQFWLPLQFLLRMGLAVTSQTGTELSLDWDKNYLLGVESITYQGQPALLLI
                     GSRELEIRDQYLPESN"
BASE COUNT          880 a          906 c          886 g          933 t
ORIGIN      
        1 catctcgcca tcgtttagtt gctgcatcct ggtataaagc tgtaaaattg gcccggggca
       61 ttgtagcccg caggcgttca ccttgatgat atccatcacc atagaaggtt cctccttttc
      121 ggagccgttt gatgcataag ccacggtggc taccacagaa tgggacgctt cacccgatac
      181 cggctgttgg cgttcttgga tcacggcatg gaaggtctta taacctccgc ttaagttgcg
      241 gacgttctca aaaccgtgtt gtcgcaagat ccggagtgcg atatatgccc tcaatcccac
      301 ctggcaataa acgatgatgg gcctatcctt tggcagctcc gccagacggc cccggagttc
      361 ggggagagga atattcaccg ccccggggag ggcccccaat tgaaattcga ctggttctct
      421 cacgtcaagc aggtagtttt ggttgccgga ttcggccagc agctgatccc aggtaatgac
      481 atccatatcc tgatttaaaa ggttggtggc ggtgtaaccc gcgatattta ccggatcttt
      541 ggcagaagag aacggaggcg cgtaagccag ctccagctcg gtcagatccc aaaccgtctt
      601 ttcgaaccgg atggcggttg ccaatacatc caggcgctta tcgacaccat cgtatcctac
      661 tacttgggct cccagaatac ggccgttatc cggagcatat aagagcttca acgtcagcgg
      721 caccgaaccc ggataatagg tggcgtggga gttggaatgg gtgatggtgc tccggtaagg
      781 aatcccccat ttctttaagg ccttctccga aagtccggtg gaaccggcgg tcaggtcaaa
      841 cagtttcacg atggccgtcc cctgggctcc gtggtaaatt tccttccgac cggttaaaat
      901 atccgcgagt aaacgaccct gatggtttgc gggacccgct aacggtaccc aactttcctt
      961 tcccgaaatg aactcccgaa tttccaccgc gtctcctaca gcatagatat ccggatcgga
     1021 ggtccgcagc gattcatcca catgaatggc tccggttgct ccgagtttta aaccagcctc
     1081 cgccgcgagt ttggtctcgg gccggacccc caccgccata atcaccatgt ctgccgtgat
     1141 cttcctgcca ctcttcaaga ttacatccag gcctttcccg gcctcactga tagcggccag
     1201 tccgtcgcct aaaatcagct cgataccatg ggagcgcaac tcctggtgaa ccggggccgc
     1261 catctcaaag tccagattgt tcaataattg atccgcgagc tccactaagg taacttgttt
     1321 tttggcctgg ttcagattct ccgccatctc cagtccgata aagccgcccc cgaccaccac
     1381 cgcggaattg accttgtgct cggtaagata ctgcttaatc cggtcggtat ccggaatatt
     1441 tcggagggta aagacttgtt gcagctgagt tccgggaatc ttcggaacta tggggtagga
     1501 accgggtgcc aggaccaatt tgtcgtaact ctccctgtaa gtccggcctt gctggagatc
     1561 tttcactacc agctcttttt tggggcggtc aatctccaga acctcatgtt tcacccgcac
     1621 gtcgatattg aagcgtttct tcattcctgc cggggtctgg acgaagagct gatcgcgctc
     1681 cgagatcacc ccaccgatat agtaaggtag gccgcagttg gcgtaggata tggcctctcc
     1741 tttttcgatc aaaatcaact ccgcctgctc atccaggcgc cgcagccgcg ccgcggtact
     1801 ggccccgccg gcaacaccgc cgataatgac atattttttt gccatccgta tcactccttg
     1861 aagattatta atcaataccc aaaataagac tgttagtgaa ttaacgaacg aatcaagagc
     1921 ttgtcggaaa tagggatcgt acgattcgtt gaatgtcttc attcactacc tggtaataaa
     1981 cctcgacccc ctggcgccgg ctccggacga ttcccaggtc ccgtaatttt cccaggtgct
     2041 gtgagacagt ggactggggg agcgccagac aatcctggat tttattgaca ttacactcac
     2101 cctgttcgat taggcctttt acaatacaaa gtcttaccgg atgggcgatg gacttgagta
     2161 attccgcctt ggcggttaac aattcaagat catcgggacg ttgcatatga ccgctccttt
     2221 tttaattata taattattat attcagataa tatgataaat gcaagacggg ttaggtctat
     2281 ggaacaatgg atggatgtca aaaacaaagt aaattggctt gggcatcaag gatatcaatg
     2341 ttttccggga tttatgtaag gcttccgtgt caagatttta tgatagattg gaagtaggag
     2401 taagattcaa ttttgtccca agcgcaatca tggtttttaa taataaaata tagtgggtat
     2461 cgaaattccc caatctcaaa atacaaggtg gtgatattcc tttgtcacat catcatcgtt
     2521 gtcgtcatca agatgatcct tgcatggaac acaggggtag gccggaagcc gtggccccag
     2581 ccggaatgac ccgaatctgc gtactggctc ccagaggtac ggcggtggcg atcgcaccta
     2641 tggcaatgcc catggggaca ccctgtggta caatgccgat gggtatgatg aatccgatga
     2701 tgcatatgga tcctatggat cggatgtgct aattcctgat atattcacat tgaattcatc
     2761 tagataaccc gagaaaaggg ccagttcatg gctcccggag gggatttacg ataggaggag
     2821 gctattacgg cttattacgc cggcctcctc ctagaaatat agatatatcg gcatgtccaa
     2881 cgtggagttt tattcagatt tttttgtcca ttaccgataa atgacttaca accaggttga
     2941 gcttcgggtt ggtatttatc ataactttct accaaagatc ttataagtaa ctctttcctg
     3001 gggttttgac ggcaggaaac accgggtgaa aatcgaagca tggatttgaa aaattataac
     3061 gaaaggcggg gcaagattgt acataaataa atggctggct ataaccgcct tgggtttact
     3121 tttgcttgcc cgaactgcgg ttgccgataa ttcgatggct ctttcctttg ccggtctgcc
     3181 ggcaggaccg gaggtgcagg ccatcaacag ggatggagcg gtttacatca atctaccctt
     3241 tcttaataaa tatttgaaca ttgtgactgc ctgggatatc tcagcgagcg acttgttttt
     3301 taaatttggc aagattagtg ttaaaatgta cgccaagaac cggagttatt atttagacgg
     3361 gagatcccaa ccctcactaa atcaagcacc atttgagcaa gacggccaat tttggctgcc
     3421 gcttcagttt ttgctgcgaa tgggtctggc ggtaacatcg caaaccggca cggaactctc
     3481 gttggactgg gataaaaact acctcttggg tgtcgaatcg ataacctacc aaggtcaacc
     3541 ggccttattg ctcatcggca gcagggaatt ggagatacgg gatcaatatc tgccggaatc
     3601 aaacc
//
LOCUS       DASVMB010000474         9819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_720000,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000474 DASVMB010000000
VERSION     DASVMB010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720000"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VF531_13255"
     CDS             complement(<1..927)
                     /locus_tag="VF531_13255"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX7714975.1"
                     /translation="MEVIAAQATSITSLNSLAHDMEATELLTRGLDIAADLSGGGLEF
                     DDPRVELAVRRVIGKGQGRIGLRDIATITQLNLSRLNLGSLSGVEQLHALSQFQAMGN
                     RLTDLEPLTGCPDLRELNLSNNRIEGLEDLAKLTKLERLHLGRNRISDLKPLTGFREL
                     RDLVLSYNQIQDLEPLAELSQLEELHLIGNQIRDIRPLARLPRLRVLSLSHNQIQDIG
                     PLEQLKHLQRLWLSDNRVEEISPLALHQQLYLLYLGSNRIRSISALQGLSGLRDLDLS
                     NNQIESLSELSALKQLQILLVSGNRIGEIGILG"
     gene            complement(991..1797)
                     /locus_tag="VF531_13260"
     CDS             complement(991..1797)
                     /locus_tag="VF531_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7714976.1"
                     /translation="MGGEVVCKIKPEEWELNRKQLELTALETELAQRELDLTTLKTEL
                     QLFQDRYLKALGDLYSRLDDLQARIAQRKGSFHPRDKHIPKEETQTGPKVENTSKDTD
                     TTKTEKTGAKRSHHFRPTEQLKKLYREVAKRIHPDLAGSEKERTRYQKLMAEANRAYR
                     EGDELQLQALLSQSGQPLESPREDEYQIRLGRIERRIGLIRERLKAIDAEIATLECST
                     LYEMRFKVEEAEKKGIDLLAEMASYLRRQIARARVELAAWNENCGEGLSA"
     gene            2070..2594
                     /locus_tag="VF531_13265"
     CDS             2070..2594
                     /locus_tag="VF531_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HEX7714977.1"
                     /translation="MNVFRKEPRITSAYLREEAKAYTERWALGRNPRYFDYSSLGGDC
                     TNFASQVLYAGGALMNYATELGWYYQTPNQKSPSWTGVNFLYQFLTGHSGPGPVAVEC
                     NPKELEIADLIQLSFTGDGVFHHTLVIVDLVKNNHPEQIRIATHSPNLLNVKLSEAYS
                     WAAIRYLHILGNQG"
     gene            2723..4006
                     /locus_tag="VF531_13270"
     CDS             2723..4006
                     /locus_tag="VF531_13270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7714978.1"
                     /translation="MNKKLYPTLAILCLVPFIMVLGNSMLIPVLPAIQKELHIGLVQV
                     GLLITVFSIPAGIVIPFAGMISDRIGRKAVMFPALLIYGIGGILAGVFAILFQEKAFI
                     YIIIARVIQGIGAGGTYQLSMALAGDLIQSTERSQVLGLLEAANGLGKVVSPLAGAAL
                     ALISWYIPFFSYGILAIPIAFLILFIVQENTEKLKANVQPIPKYFQNLKKIFKEKGIA
                     LIISFASGFMVLFALFGLLSFFADILEKQFKIGIFQRGLVLAVPVLLMAVTAFILGTV
                     MQKHLAKILKWAVVAGLILVGAGLFLFTPCRKPLSFTLSAAVLGLGTGAVLPSLNTLI
                     TSSAPKTERGLVTCLYGTVRFFGVAVGPPLFGLAEKITKPPVFYTTAAVCAGLGILFC
                     ILVQPGKIIPKDIQGQTKSGSKPNQKQPAKSRKKS"
     gene            4134..5066
                     /gene="corA"
                     /locus_tag="VF531_13275"
     CDS             4134..5066
                     /gene="corA"
                     /locus_tag="VF531_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX7714979.1"
                     /translation="MLYIWHPDQPLEVVSNSSFKNAVADRSAVVWLDLEGDPDSLSQE
                     LTGLDSLHPLTIERLFTPGPRAFLEEFDDYLHILLQEIHYHQTYQVCHFILGSNYLIT
                     IHRPNFYWPEKTNPPSRFFSQGSDILFYYLVQPIIEAGFEALDTVADITEELEDKIFS
                     RPDNQLLNELFNLKRELIEIRKALAPMREVFALISRRDNPFVDDQALPFMSHHYDQLI
                     RLHEISDSQREIVASALEIYLSSISNRMNEIMKTLTIVSTIILPATLVVGFYGMNFQH
                     FPGLSWKYGIWCVLSLILIGTAGMLWYFKRKKWL"
     gene            complement(5061..5708)
                     /locus_tag="VF531_13280"
     CDS             complement(5061..5708)
                     /locus_tag="VF531_13280"
                     /inference="COORDINATES: protein motif:HMM:NF021093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEX7714980.1"
                     /translation="MFRYRFFVRIITAGLIVALFLLIGFAGTGTALTRPIIEAYNRTN
                     LIRLHVVANSDSPLDQAVKLKVRDRIIEVTEPLLLRVEDPQKAERILQNHLELLKETA
                     SEELRRNNRSMPVQVSVGQFSFPERAYPFGILPAGDYKGVRIVLGKGLGKNWWCVLYP
                     PLCLLDPDAPSSRNTNRQKPVKIQYRLATLERLLQQKGLAMNGFWQGWGKYFGLL"
     gene            complement(5729..6523)
                     /gene="sigG"
                     /locus_tag="VF531_13285"
     CDS             complement(5729..6523)
                     /gene="sigG"
                     /locus_tag="VF531_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HEX7714981.1"
                     /translation="MLVNKVEICGVNTAKLPVLSSNKMRELLTRLQAGERSAREELIN
                     GNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKAIDNFDLSQNVKFSTYAVPMIIGEI
                     RRYLRDNNPLRVSRSLRDIAYKALQVRDALISRNSKEPTVAEIAEELKVPREEVVFAL
                     DAIQEPISLFEPIYHDGGDPIFVMDQISDERHMDGQWLEGLSIKEAMSKLNDREKLIL
                     EMRFFEGRTQMEVAEEIGISQAQVSRLEKTALKHMKRYISGTNDKE"
     gene            6860..6942
                     /locus_tag="VF531_13290"
     tRNA            6860..6942
                     /locus_tag="VF531_13290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6894..6896,aa:Leu,seq:gag)
     gene            complement(7542..7862)
                     /locus_tag="VF531_13295"
     CDS             complement(7542..7862)
                     /locus_tag="VF531_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714982.1"
                     /translation="MRSVEIKKIGAGSAFQLCFTIGAMAGFVICLIFLITGSLLQNIG
                     MELGTSLGSGGPLTIGAAVAGVLIGSLTYGLASGILAAAGAFIYNLFAAAVGGIRIQI
                     DIND"
     gene            complement(7956..8831)
                     /gene="hisG"
                     /locus_tag="VF531_13300"
     CDS             complement(7956..8831)
                     /gene="hisG"
                     /locus_tag="VF531_13300"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7714983.1"
                     /translation="MTTQLKLGLPKGSLQDSTLRLFKKAGYNITVSERSYYPTIDDTE
                     LETMLIRAQEMSRYVEEGVLDAGITGNDWILDNHSQVVPVADLLYSKQTLNPCRWVLA
                     VPNDSSFQKAADLDGKRVATELLHVTADYFAKFGVNPEIEFSWGATEVKPPVLADAIV
                     EITETGSSLRANKLRIIDTVLAVKNQFIANQKAWEDPWKRQKIENLAMLLKGGLLAEG
                     KVGLKMNVEKKDMEKVLKILDSLKKPTISQLSDPDWIALEVISDEKVVRDLIPQLKRA
                     GAQGIVEYPLTKVIY"
     gene            complement(9161..>9819)
                     /locus_tag="VF531_13305"
     CDS             complement(9161..>9819)
                     /locus_tag="VF531_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7714984.1"
                     /translation="DETTIVDGRGDIAGIKNRINQIKVQIEDTTSDYDREKLQERLAK
                     LSGGVAVIQVGAATETEMKEKKHRIEDALSATRAAVEEGVVPGGGVALLQIAPVLDGI
                     TETGDVATGISIVRKALFEPLRQIANNAGVEGSVIVERVSGMEQGQGFNALTGEYMNM
                     IAAGIVDPAKVTRSALQNAASIAAMLLTTECLETEIPEKEKPTLPGGGGGMPGGLGMD
                     "
BASE COUNT         2362 a         2645 c         2220 g         2592 t
ORIGIN      
        1 ccctaaaatc ccgatctcgc caatccggtt tccggaaacc agtaagattt ggagctgctt
       61 aagagcggaa agttcggaga gggactcaat ctgattgttg gaaagatcta gatcccgtag
      121 tccggatagc ccttgtaagg cactaatgga ccgaatccga ttggaaccaa gatataacag
      181 atataattgc tggtgaaggg caaggggtga gatctcctcc acccggttat cactaagcca
      241 gagacgctgc aaatgtttga gctgctccaa aggtccgata tcttggatct gattatgaga
      301 tagagataag actctgagcc ggggcaaacg agccagaggg cgaatgtccc tgatctgatt
      361 cccgataagg tgtagctctt ccaattgact cagttcagcc aatggttcca gatcttgaat
      421 ctgattatac gaaaggacca aatcccgtag ctccctaaac cctgtcaaag gtttcagatc
      481 gctgatccgg tttctcccca gatggagccg ttccagtttg gtaagtttgg cgagatcctc
      541 taatccttca attcgattat tggagaggtt aagttccctc aaatccgggc aaccggtcaa
      601 gggttccaga tccgtgagcc ggtttcccat cgcttggaac tgcgacaagg catgaagctg
      661 ttccactccg gagagagaac cgagattcag ccgggacagg ttgagttgtg ttatggtcgc
      721 aatatcacgt aaaccaatcc ggccttgccc tttaccgata acacgcctta ccgccagctc
      781 gacccgggga tcatcaaatt ccaacccgcc accgctcaag tccgcagcga tatccaaacc
      841 ccgggttagt aattccgttg cttccatatc atgggccaga gaattaagtg aggttatgct
      901 ggtggcctgt gctgctatga cttccatctt taccgactcc attttgttcg aattcgatga
      961 gacgatccgc gcgcactgcg cgaatattcc ttaagcggag agtccttccc cgcagttctc
     1021 attccaagcc gctagttcga ccctggcccg ggcgatctga cgcctgaggt agctcgccat
     1081 ttccgccagg agatcaatcc cctttttctc ggcttcctcg actttaaacc gcatctcata
     1141 caatgtcgaa cattctaaag ttgcaatctc cgcatcgata gcttttagcc gctcccggat
     1201 taacccgatt cgccgctcta tccttcccaa ccgaatctga tattcatcct cccggggtga
     1261 ttcgagtggt tggccgcttt gactcaaaag ggcttggagc tgtagttcgt ctccttcccg
     1321 ataagcccgg ttggcttcgg ccattagttt ttggtatcgg gttcgttctt tctcactacc
     1381 ggccagatcg ggatggatcc gtttcgccac ttcccggtag agtttcttca gttgttcagt
     1441 gggacggaaa tgatgtgatc ttttagctcc agtcttttcg gttttggttg tatcggtatc
     1501 cttcgatgtg ttctctacct tgggcccggt ttgggtttct tccttcggga tatgtttatc
     1561 ccgcggatga aagctccctt tgcgttgagc gatccgggcc tgtaggtcgt ccagtctgga
     1621 ataaagatcg cccagggcct tcagatatcg atcctgaaat aactggagtt ccgttttaag
     1681 ggttgtgaga tccaattctc tctgagccaa ttcggtttcg agcgcagtca attccaactg
     1741 tttccggtta agttcccact cttccggctt gatcttgcaa acaacctctc cgcccatgaa
     1801 caacccctcc gtggtaacca ctgacgatta acgatacaat caacactttg cctattgttg
     1861 tttcggtctt ttattatatc ggccgtaatg ccgcgaactt tagccatttt tcgcattcaa
     1921 tccccacttt tacaagccct tttggtttat ccggactgat taaccgcgtc ggccgatgag
     1981 ttccgataat cgctttctgc aggggcgcgc gcacaccctc acgcatattg tgaaagtaaa
     2041 actgaaaacg aacgggaggc tgtatcgatt tgaacgtttt ccgtaaagaa ccccgcataa
     2101 cctccgcata tctgagagaa gaagccaaag catatacaga acgttgggcc ctcggccgta
     2161 acccgcggta ctttgattat tcctcattgg gcggtgattg cacaaatttt gcttcccaag
     2221 tgttatacgc agggggagcg ttgatgaatt atgccaccga actaggctgg tattaccaga
     2281 cccccaacca gaaatcaccc tcctggaccg gggtaaactt cctttaccag ttcctcaccg
     2341 gtcattccgg tcccggaccg gtggcagtag agtgcaatcc caaagaactc gaaatagcgg
     2401 atctaatcca actctctttc accggtgacg gggttttcca tcacacactg gtgatcgtcg
     2461 atctggtaaa gaacaatcat cccgaacaga tccggatcgc cacccattca ccgaacctac
     2521 tgaatgtcaa actaagcgaa gcgtatagtt gggcggccat acgttacctc catattctgg
     2581 gcaatcaagg ttgaaccatg tttcacggag tgaaccggag tttttcaaaa cggatcggtg
     2641 catactaaaa aagtccgcgt aaaaaaaagg acaagccaag ccagaaaaaa cccaaccact
     2701 tgtttatgga ggaaatccat ccgtgaataa aaaactctac cccactctgg ccattctttg
     2761 cctcgttcct ttcatcatgg ttttaggcaa ctcgatgctc atcccggtgc tcccggccat
     2821 tcaaaaggaa cttcacattg gactggtcca agtgggatta ctgattacag ttttctcaat
     2881 tcctgccgga atcgtcatcc ccttcgccgg gatgatctcc gatcgaattg gtcgcaaggc
     2941 cgtgatgttt cccgcgcttt taatctacgg aatcggcggt atccttgccg gggtcttcgc
     3001 gattctcttt caagaaaagg cttttatcta catcattatt gcccgggtca ttcaagggat
     3061 cggcgccggt ggcacgtacc aattgagcat ggcactggcc ggagatttga ttcaatctac
     3121 agaacgctct caagttctag ggttactcga agcagccaac ggattaggaa aagtcgtcag
     3181 cccgctggca ggggccgctt tggccctaat atcctggtat ataccttttt tcagttacgg
     3241 gatcctggca atccccattg ccttcctcat attattcatt gtccaggaaa acacggagaa
     3301 attgaaagcc aatgtccaac cgatccccaa atattttcaa aacctaaaaa aaatcttcaa
     3361 agaaaaaggc attgccctca tcatctcttt tgccagtggt tttatggttc ttttcgcgct
     3421 ctttgggttg ttgagtttct tcgcggatat tctggagaag caatttaaga tcgggatctt
     3481 tcaaagggga ctggttctgg cagtgcccgt actgttgatg gcggtaacag ccttcatcct
     3541 gggtaccgtg atgcaaaaac accttgccaa aatccttaaa tgggccgttg tggctgggtt
     3601 aatcctcgtg ggggcggggt tattcctctt taccccctgc cgtaagccgc tcagctttac
     3661 cttgagtgcg gcagtattgg ggctaggcac cggggcggtt ctgccttcac tcaatacatt
     3721 gatcacctcc agcgctccca aaaccgaacg gggtctggta acatgccttt acggcacggt
     3781 ccgtttcttt ggagtagctg tgggacctcc ccttttcgga ctggcagaga agattaccaa
     3841 acctccggtc ttttacacta ctgccgcagt ctgcgccggg ctggggatac tcttttgcat
     3901 cctggtgcaa cccggcaaaa tcatacccaa ggatattcaa ggccaaacca aatccggatc
     3961 aaaaccgaat caaaaacagc cggccaaatc cagaaaaaag tcctgaggcg aggatccgga
     4021 ggctgcaatt aaccaagcct atcggcggat gttaaagtcc caagtctgac caggagcagg
     4081 agccgtacct tcagtggcga attatttccc aatgttctct ggaggtgaag atcttgctct
     4141 acatctggca cccagatcag cctttggaag ttgtctccaa tagctctttt aagaatgccg
     4201 ttgccgatcg atccgccgtg gtctggctgg atctggaagg cgaccccgat agtttgagtc
     4261 aggaactgac cggacttgat tctctccatc cgttgacgat cgagcggctc tttaccccgg
     4321 gtccccgggc ttttcttgag gaatttgacg attatctcca tatcttgctt caggagattc
     4381 attaccacca aacctatcaa gtctgtcatt tcattctggg aagtaattac ctgattacaa
     4441 tccatcggcc caatttttac tggccggaaa aaacaaatcc cccaagccgc tttttcagtc
     4501 aggggtcgga tatccttttt tactatttgg ttcaaccaat cattgaggcc gggtttgagg
     4561 cgctcgacac agttgcggat atcaccgaag agcttgaaga caaaattttt tcccggcccg
     4621 ataatcaact actcaacgaa ctatttaacc taaaacggga attgatagag atccgtaaag
     4681 ccttggcgcc gatgcgcgaa gtatttgcct taatttcacg gcgtgataac cccttcgttg
     4741 acgaccaggc cttgccgttt atgagtcatc actatgatca gctgatccgg cttcacgaaa
     4801 tcagtgattc tcaacgcgag atcgttgcta gtgccctgga aatctacctg tcttccatct
     4861 ccaaccggat gaacgagatc atgaaaacgc ttacgattgt ctcaaccatc atcctgccgg
     4921 ccacattagt cgtgggtttt tacggtatga actttcaaca ttttcccggc ctatcttgga
     4981 aatacggtat ctggtgcgtc ctatccctca ttctcatcgg aaccgccgga atgctttggt
     5041 attttaaacg caagaaatgg ttataaaagt ccgaaatatt taccccatcc ctgccaaaaa
     5101 ccattcatcg ccaaaccttt ctgctggagc agtctttcca aagtggccag gcgatactgg
     5161 attttgacag gcttttgacg gttcgtgttc ctagaggaag gggcatccgg atctagcaga
     5221 cacaagggcg gataaagtac gcaccaccaa ttttttccca accctttccc caggacaatc
     5281 cgtactcctt tatagtcgcc tgccggaaga atcccgaatg gataagcacg ctccggaaag
     5341 gaaaactggc ccacacttac ctggaccggc atggaacggt tattgcgccg cagttcttca
     5401 ctggcggtct ctttcaaaag ttccagatgg ttctgcaaaa tccgctccgc tttttggggg
     5461 tcttcaaccc gtaaaagaag cggctccgtg acttcgatga tccggtcccg aaccttcagt
     5521 ttaaccgctt gatcaagtgg cgagtcgcta ttggctacga catgaagccg gattaaattg
     5581 gtccggttat atgcctcaat gattggtcgg gttaacgcgg tgcccgtccc ggcgaaaccg
     5641 atcaacagga aaagggctac aatcaatccc gctgtaatga tccgtacaaa aaaccgatat
     5701 cgaaacaagt tgaagcctcc taaaattctc attctttatc attcgttccc gaaatataac
     5761 gtttcatatg cttaagagcg gtcttttcaa gccgcgagac ctgggcctgg gagatcccga
     5821 tctcctcggc cacctccatc tgggtccgtc cctcaaaaaa acgcatctct aaaatgagtt
     5881 tctccctatc gttcaatttt gacatggctt ccttaatcga gagcccttcg agccactgcc
     5941 cgtccatatg ccgttcatca ctaatctgat ccattacaaa gatgggatcg cccccatcgt
     6001 gatagatagg ctcaaatagt gagatcggtt cctgaatggc atcgagtgcg aaaaccactt
     6061 cctcacgggg aacctttaac tcctcggcga tctccgccac tgtgggttct ttggaatttc
     6121 tgctgattag ggcatcccgg acttgtaagg ctttgtaagc gatatcccgc agtgagcggc
     6181 tgacccggag cggattatta tctcgcaaat agcggcggat ctcgccgata atcatcggta
     6241 cggcatacgt tgaaaacttc acattctgcg agaggtcaaa gttgtcgata gccttcatca
     6301 aaccgataca gcccacttga aagagatcat ccacgtattc gccgcggttg ttaaaccgtt
     6361 gaatgacact taaaaccaaa cgcagattcc cgttgatcaa ctcttccctg gccgaacgct
     6421 ctccagcctg tagcctggtc agaagctccc gcattttgtt gctggagaga accggaagct
     6481 ttgcggtatt tactccgcag atctcaacct tattcaccaa cattcgatca atcaactccc
     6541 gcctgttgca acgagtgttt tgcgtacgcg cacgatcatc gatcattatc aggacttccc
     6601 ggaacatttt aggccgacat ttcaatcact atccgggtag aatgccctca aatgcttaca
     6661 tgatgcgtct acgaaagatt attgccacgc atggcgggtt ttatactaga gagacagcgc
     6721 cgttaccggg gtggaagtga aacacgagct caggaaggtt ttacaaatag cctatctgtc
     6781 tggtaattcc ttgaaagtgg ataaagagcg gttgacaatt tgatgaagag accttataat
     6841 attaaatagt cgatttcgtg cccaggtggc ggaactggca gacgcgtacg tttgaggggc
     6901 gtatgggcaa ccgtaccggt tcaagtccgg tcctgggcac caaattattt tcagattaaa
     6961 atttcatttt tgaacgtcgt acttgacacc gaatgctttt tcgcattccg gtgtttttta
     7021 tttttagtca ctgaaaaatg accttttgac gcccattcat gtccgggtca gcctgggcac
     7081 gctatcatcc tgccgattac atcataacca tgggtgtaac caagcaaggg tctgtctcaa
     7141 aagtccgatc tgtcattccg tccacccggg gtgcgcgtcc catgggacgc gagaaacccc
     7201 gggctgagtt aaaaaccatt ctaaagcccg ttaaaacggg ctgggaagaa ccggaggcat
     7261 tacccagccc cattcatggg gcttgagatg atttttccag gcatagccag gtcctttagg
     7321 gcctggtggg gataagggcg gttatccatt gtttgctttt aagacagacc cttgctcatt
     7381 caatcgcatg aatagacgga ctgattcttc cctattttaa tgttgatgaa ccattcgcgc
     7441 ttgttgatgc cgggccagcg atacagaaga ccaaactgat tcataaaaaa agaacaacta
     7501 aaagttgttc cttttcgcgg gtttattcaa gatttatttt gtcaatcatt gatatcaatc
     7561 tggatccgaa ttcctcccac cgccgcggcg aagagattat agatgaacgc gccggcggca
     7621 gccaggatgc cgctggccaa accataggtc aaactgccga taagcactcc cgcgactgcg
     7681 gctccgatcg ttaacggtcc tccggagcca agcgaagtcc caagttccat cccgatattc
     7741 tgcaataagc tcccggtaat cagaaagata aggcagatca caaaaccagc catagcaccg
     7801 atagtgaaac agagctgaaa agcggatcca gccccgattt tttttatttc cacgctccgc
     7861 atccataacc cctcccctca tcagatctta tgggaaggaa ttcatctata gacccgtaga
     7921 gattcatttt ctctgctatc ctcatcaccc cggttctagt agatcacctt tgtcaaggga
     7981 tactccacaa tcccttgtgc tccagccctt tttaactgcg gaatcagatc ccgtaccacc
     8041 ttttcatcac tgatcacctc aagcgcaatc caatccgggt ctgaaagttg cgatatcgtg
     8101 ggctttttca atgaatccag tatcttcagg actttctcca tgtccttttt ttccacattc
     8161 attttgaggc cgacttttcc ctcagccaac agtcctccct tgagcagcat cgctaaattc
     8221 tcgatcttct ggcgtttcca tgggtcttcc caagcctttt ggtttgcaat gaattgattt
     8281 ttcaccgcaa gcactgtgtc gatgatacgc aacttatttg cccgcaacga gctgccggtc
     8341 tccgtgatct ccacgatggc atcggccaat accggcggtt tcacctctgt ggctccccag
     8401 gagaattcta tctccggatt tacaccgaat ttggcgaaat aatccgcagt tacatgaaga
     8461 agttcagtgg cgactctttt gccatcaagg tccgcggctt tttgaaaaga ggaatcgttg
     8521 ggaacggcca aaacccagcg gcacggattc agggtttgct tggagtataa aagatccgct
     8581 accggaacca cctgggagtg gttatcaaga atccaatcat ttcccgtaat tccggcatcc
     8641 agaacgccct cctcaacata acgcgacatc tcctgggccc ggataagcat ggtctccagt
     8701 tccgtgtcat cgattgtagg ataatatgaa cgctcgctta cagtaatatt gtaaccggcc
     8761 ttcttgaata accgcagtgt ggaatcctga aggctgccct tgggcagacc taatttcaat
     8821 tgcgtcgtca tgaatcatca acctcccaat gaaaaatttg accacagaaa acccgtcccg
     8881 acgattgaag aactgattcg ccgcaatggt ctttttttcc tgtgatgcac taaaaaatta
     8941 tgcggaaaac tgctattttc gactcactct cgctctctct ggcggcaagc gccaaagtcg
     9001 cgcacatgtg cgcgcgtaac ccagatcctc cgttgcctat tgcactgctc cgaatccgcc
     9061 ttctcccctc tttcccgctt gccgggagag agggggcaag ggggtgagtc gcagcatatc
     9121 ataaaaaaat ccggccgcag ttagccggat ttttcaagtg tcaatccata cccagtccgc
     9181 cgggcatgcc gcctccacca ccgggaagag tgggtttttc cttttccgga atctccgtct
     9241 ccagacattc cgtagttaga agcatcgccg caatactcgc cgcattttgc agggcactcc
     9301 gagtgacttt ggccggatcg acgattccgg cagcgatcat attcatatac tccccggtca
     9361 aagcgttaaa cccttggcct tgttccatgc cgcttactct ttccacaatt accgaacctt
     9421 ctacgccggc attgttggct atctgccgta gaggctcgaa taatgctttt ctcacaattg
     9481 agatcccggt agccacatca ccggtttctg tgattccatc aagtaccgga gcgatctgca
     9541 aaagcgctac accgcccccg ggaacaacac cttcctccac agcggcccgg gtcgcggaga
     9601 gcgcatcttc aatccggtgt ttcttctctt tcatttctgt ctcggtcgcc gcgcctactt
     9661 gaatcacggc gactccaccg gaaagtttgg ccaaacgttc ctgcagcttc tcccggtcgt
     9721 aatcggaagt agtatcctcg atctgtactt tgatctgatt gatccgattt ttgatcccgg
     9781 caatatcccc cctgccatcc acgatagtgg tttcatcct
//
LOCUS       DASVMB010000475        10852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_648756,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000475 DASVMB010000000
VERSION     DASVMB010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648756"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VF531_13310"
     CDS             complement(<1..310)
                     /locus_tag="VF531_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7714985.1"
                     /translation="MEVQERRARAVKLKEHVIANLLLTVACVAVAGVILIIFFVFREG
                     WPVLMKYGPFHLASGKEWLPTSNYFGLLPLIVGSAYVTLTALVFGVPLGVGCAVFMAE
                     I"
     gene            complement(356..1159)
                     /locus_tag="VF531_13315"
     CDS             complement(356..1159)
                     /locus_tag="VF531_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7714986.1"
                     /translation="MRRAVYTFVLMTIITGSLFFSGCANRSRIDIVGSTSVQPIAEAL
                     AEGYMESHRDVNINVQGGGSSAGIKAIKDGTADIGTSSRPLAPAEQGKDLKPVTIAWD
                     GIAIIVNSQNPVAGLTGEQIRGVFAGTIRNWSQVGGANRPIMVVNREEGSGTRTAFTE
                     LVMKETPLVAKTIVQSSTGAVRQTVAGNPDAIGYISLDAVNSEIKALAVDGVVCNAGN
                     IQTKRYPITRPFLFIIDANKTDKAVARFVDFVLHDGQAIVIQNGLVSVK"
     gene            1462..2085
                     /locus_tag="VF531_13320"
     CDS             1462..2085
                     /locus_tag="VF531_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7714987.1"
                     /translation="MFRITSKEEIFFDLFVETSEITCQAAAKLDQLMSDYVATPERIK
                     EMEKIEHQCDQQVHQILAQLNRSFITPIDREDIYAIAKELDNITDDIESTAHRFYMLN
                     VKNIREDAKKLAHLIVDCTNELNLVMADLKNMKRSTTLAKRIIEVNRIENDGDEVFRN
                     AITQLFATETNALEVIKWKEIYEFLENTLDACEDVANLAEGVIMKNA"
     gene            2078..3070
                     /locus_tag="VF531_13325"
     CDS             2078..3070
                     /locus_tag="VF531_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7714988.1"
                     /translation="MLSTVLVIVILLALCFDFINGFHDTANSIATSVSTRVLSPRAAI
                     VMAASLNFVGALISDKVAKTFSNGLVDSSLVVEKIVIATLIAAIIWDLITWYIGFPSS
                     SSHALFGGLIGAAVAYTGSIHVVIWDNVLRIVLLFLASPILGFSLGYLFMSILYEILR
                     PFSQRTVNQWFSKLQILSAAWMSYSHGGNDAQKSMGIITLALISAKIITPSHGVPIWV
                     MVLCATAMALGTSIGGWKIIKTVGVNMIRLQPINGFAAETGAALVIEGASSIGVPLST
                     THVISTAIMGVGASKRVSAVRWALAKNIVWAWVLTLPITALLGAGIAMIFKLIG"
     gene            complement(3219..4970)
                     /locus_tag="VF531_13330"
     CDS             complement(3219..4970)
                     /locus_tag="VF531_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7714989.1"
                     /translation="MKNSAWLKKILYRPIRYYLDLKIVWKLLISFGLIWAIVLLVVAV
                     SQNGIQKLNGTLESLIHSELEPLVYLNDIRSNVTELEIETKNAMLQKDRTSLLYIGNA
                     LLPNTISKNIDYSFTQLLKIAAPGKEATNLGEIRNYWKLYEKIYLRVLQNPDSYLEPA
                     VQTEISRLRYFLIGGIDRMNENYYRQRAVLAQVEANRIYLRQQYTTFSLLAVAFILVI
                     GISVLTAWMIITPLRRMSAASRKIAAGELKVILPEDRRDEFGEVCRCFNMMSNELALL
                     AEQIKRVADQVSLSSHRLQDGANITTGAAQQLLDTMAEVAAGAETQEQQVAAIREVIQ
                     TATQFSQMVDDITGKAAGLAEDTVDKAVKGEEAAVVVGGQIQRIQRFMAQSEHTMERL
                     QSLSTEIENMVDTVQNIAEQTALLSLNASIEAARAGEFGRGFGVVAKSIGKLSEQTKA
                     TAETTQDLVERIQQLFGDLNQMIQDETQMIHDSEQATAVLNEVFQSISNATGRVNRSL
                     GEVTGNTRQLVQKYQDILQAVSRITEIALHHKEGTEQASSAAGEHFSCSQEIISTSNE
                     LARWGDQLLQTVNRFRI"
     gene            complement(5201..5332)
                     /locus_tag="VF531_13335"
     CDS             complement(5201..5332)
                     /locus_tag="VF531_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714990.1"
                     /translation="MICPDCKATVLSFDEYCKKCGADLYTTKQVSQLKKVKGVYRAF"
     gene            complement(5497..6303)
                     /locus_tag="VF531_13340"
     CDS             complement(5497..6303)
                     /locus_tag="VF531_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7714991.1"
                     /translation="MKKVIALVIAGIICLSAFGFAAPFSGTIQIVGSTSVQPLAEELA
                     QAFMAKNKGVKVFVQGGGSGAGIKAAVDGTADIGSCSRELESAELQTGIVETVIARDG
                     IAVIVSKSNSVNNLTTEQLQKIYSGQITNWKEVGGPDQKIQVVNREAGSGTRGAFEEL
                     LLKPAKLENTSNCLVQASTGAVQETVAVTKEAIGYVSLGFLDTKSVKAVTIDGIQATD
                     KNVLAKTYKLQRPFLLLTKTKPAGLTKTFLDWITGPQGQKIVAKDYIRIK"
     gene            6580..6798
                     /locus_tag="VF531_13345"
     CDS             6580..6798
                     /locus_tag="VF531_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7714992.1"
                     /translation="MLVAILKVADGLDHTRQSNVSSVQCIIEPIQIKLLCHPRHSGSE
                     EDLQSARQKGGFFEKVYHRKLMIEWELA"
     gene            6911..7573
                     /locus_tag="VF531_13350"
     CDS             6911..7573
                     /locus_tag="VF531_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7714993.1"
                     /translation="MELTLNIIKKDPLITAFIQKADEHLAAMGFTEHGFRHVSLVSSI
                     SQNILLRLGYPDHQAELAAIAGYLHDIGNTVNRKDHGRTGALMALNYLLSNTMDPSDA
                     ATIAGAIGNHEEEYGEAVNVIAAAVILADKSDVHRSRVRNADLATFDIHDRVNYAAVH
                     SFLNVNSEKKSITLELTIETAICPVMEYFEIFLVRMMMCRRAAVFLNCNFKIEINGAQ
                     IL"
     gene            complement(7808..9007)
                     /locus_tag="VF531_13355"
     CDS             complement(7808..9007)
                     /locus_tag="VF531_13355"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoform II"
                     /protein_id="HEX7714994.1"
                     /translation="MKVFLKAFFIMVLLASLTFPVLARNFDLPQPSGPNSVGTMRLGF
                     VDPSRKGIFTSNPDEYREIAVQVWYPTTDDLQGKEPLFYIPDRTVGKYIAKRLGMPNV
                     FGKLAAVRTNSYLDAGLSAGTDKYPVVVFSAGYGNIGNANTVQMEELASHGYVVFSIS
                     HTYEDVASIFPDGRIAYFDPKQMKVFTDDLLRAAKETKAKKPENPVKYGIEQSKMAYQ
                     SVHIWSDDTSFVVDQIEKLASGEIKSIFEGRLDVANMGVFGHSFGGATAGQVCITDFR
                     FKAFINLDGSPFGDIINNPVKQPFMLITADGREIPLTAGYSPEQTNYLVVYIKGATHS
                     DFMDFSILFPVFRYIGMFGKIGGERNSQIVNDYVTAFFNKYLKGKEEPLIDQMIFKYP
                     EVRLEAK"
     gene            complement(9092..10534)
                     /locus_tag="VF531_13360"
     CDS             complement(9092..10534)
                     /locus_tag="VF531_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7714995.1"
                     /translation="MKDKKSIGRFLFRNSVVIVLIALVIFFIARFICNTISEILIFKY
                     LNWEYIGSEKTYTYPFDRMNTKSIEAVGGWIEILDENRRVIFVRGTKRDQIYQYDQNQ
                     LFDDAAILHRDTGRYPFIYNIYPVKGPAGKDYLFIIKLPKSLYHVSITANFWAVFSHP
                     NRLHIIAWVAISGCFLILFLAGMYIYSKFLAKHIRKPLGYLMDGIKEMEKQNYRFRLN
                     FYAEREFAWIRDAFNEMITRLEKAESEKRRLEESKQRMLVDISHDLKTPITSICGFSK
                     LLHDGEIDAAKDREKYVTYIYNKAHYVAGLVDDLFELSKLEDENFQFAYSRNNLAEWL
                     RQLVGEIYPEFERKKFILDIDIEEKPIMVDFDPIQMTRAVNNLLYNVLKHNPEQTRVR
                     IRCRREESQAVLQIADNGIGIPAELRDIIFDPFVRGDEEGSAKDGTGLGLAIAKKIIQ
                     RHNGTIILSSAEEAVTIFTIGLPLADLRLH"
     gene            complement(10531..>10852)
                     /locus_tag="VF531_13365"
     CDS             complement(10531..>10852)
                     /locus_tag="VF531_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7714996.1"
                     /translation="NQAGECIKFGDWILRQDSCELYKNDAKVELTSMEYKLLLFLITN
                     PNRVFTKQQLYERVWGQEFMGDENIIMVYISKLREKIEGDPKNPRYLKTIRGLGYRFE
                     GEIQ"
BASE COUNT         2794 a         2809 c         2320 g         2929 t
ORIGIN      
        1 ctatctccgc cataaacacg gcgcatccca cgccgagcgg caccccaaat accaaagccg
       61 tcagcgtgac ataggccgaa ccgacaatca acggcaacaa cccaaagtaa ttcgatgtgg
      121 gtagccattc cttgcccgac gcgagatgaa agggcccgta tttcatcagg accggccaac
      181 cctcccggaa aacaaagaaa ataatgagaa tgaccccggc caccgcaacg caggcgaccg
      241 tcaacagcag attcgcaatc acatgttctt tcaatttgac cgcgcgcgcg cgcctctcct
      301 gaacttccat gagcgcctcc tggaaaaagg taaaaagccg gttgatttgt gaaatctact
      361 taactgagac caacccattt tgaatgacga tcgcctggcc gtcatggaga acgaaatcga
      421 caaaccgggc caccgcctta tctgtcttgt tggcatcgat aatgaaaaga aaaggccggg
      481 taatcggata acgtttcgtt tgaatattac cggcattaca aacaaccccg tcaaccgcca
      541 acgccttgat ctccgaattc accgcatcaa gggaaatata accgatggca tccggatttc
      601 cggctaccgt ctgtcttact gccccggtag aactctgaac gatagtcttg gctactagcg
      661 gagtctcttt cataaccagt tcggtaaaag cggtccgggt tcccgaaccc tcctcgcggt
      721 tgaccaccat aatcgggcga ttcgccccac ccacctggga ccaattcctg atggtcccgg
      781 cgaagacccc gcgaatctgt tcgccggtta gaccggcgac cgggttctgt gaattgacga
      841 tgatcgcaat gccatcccag gcaatcgtga ccggtttgag atccttgccc tgctccgcgg
      901 gagcgagagg tcgggaactg gttccgatat cggcagtgcc gtccttaatt gccttgatcc
      961 cggcgctgga acctccgccc tggacattga tattcacatc ccgatgagac tccatatatc
     1021 cctcggccaa tgcctcggcg atgggttgca ccgaggtaga accgacaata tcaatccggc
     1081 tccgattcgc acaaccgcta aaaaatagag atccggttat gatagtcatc aatacgaatg
     1141 tataaactgc tcgtcgcatg gatgatcact cctttgagat caatgctaac gaaccgggtt
     1201 taagccgggt ttatccacag attaagaatt tgttaaggac aagccgtccc ctcttcatgt
     1261 gctgttcgcg gatcgcggaa atagaggctg agattgtatt cagtactgga gtagtatata
     1321 atcaagtagg attttggaaa tcggttttaa ttttgaaaac caaattttca cttcaaggac
     1381 ttcatccctt gaaacaaggg atttttatgc gttgcaaggg ttacgataaa aaaattaatt
     1441 accggaaagc gagggtgccc aatgtttaga atcacatcca aagaagaaat cttttttgat
     1501 ttattcgttg agacctccga aattacttgt caagccgctg caaaactgga tcagttgatg
     1561 tccgattatg ttgctactcc ggaacggatt aaggagatgg aaaaaattga acaccaatgt
     1621 gaccaacagg tccaccaaat tttagcccag ctaaaccgtt ctttcattac ccccatcgat
     1681 cgcgaggata tttacgcgat tgccaaagag ttggacaaca tcaccgacga cattgaatcc
     1741 accgctcacc gcttttatat gttgaacgtc aaaaacatac gggaagacgc caaaaagttg
     1801 gcccatttga tcgtggactg caccaatgag ttgaatctgg ttatggctga tttgaaaaac
     1861 atgaaacgca gtaccacact ggccaaacgg atcatcgagg tcaaccggat tgaaaatgat
     1921 ggcgatgagg tcttccgcaa cgccatcact caattattcg ccaccgaaac caatgccctg
     1981 gaagtcatca aatggaaaga aatttacgag ttcttagaga ataccctgga tgcctgcgag
     2041 gatgtggcca atcttgccga aggggttatt atgaaaaatg cttagtaccg tactggtcat
     2101 tgtaatccta ttggcgctat gcttcgattt tatcaacggt ttccacgata ccgccaactc
     2161 cattgccacc tcggtctcga cccgggtact ctcgccacgg gcggcgatag tcatggccgc
     2221 ttccttgaat tttgtgggcg ccctgataag tgataaagta gccaaaacct tctcaaacgg
     2281 cctggtcgac agtagtttgg tggtggagaa gatcgttatc gccacgttga tcgccgccat
     2341 tatctgggat ttgatcacct ggtatatcgg ttttcccagc agttcctctc atgcgctttt
     2401 cggcggattg atcggtgccg ccgtcgccta taccggcagt atccacgtag tgatctggga
     2461 taatgtgtta cggatcgtcc tccttttctt agcctctcca atcctcggct tctctctggg
     2521 ttatctcttt atgtccatct tatacgagat cctccgtccc ttctcccaga ggacagtgaa
     2581 tcaatggttt tccaagctac agattctctc tgcggcttgg atgtcctact cccacggtgg
     2641 aaacgacgcc caaaagtcga tgggtatcat caccctggca ctgatcagcg caaaaatcat
     2701 cactcccagt cacggggttc caatttgggt aatggtcctc tgtgccacag cgatggctct
     2761 cggtacctcc atcggtgggt ggaagatcat caaaacggtc ggtgtcaaca tgatccggct
     2821 ccaacccatt aacggttttg cggccgaaac cggcgccgcc ctggtgatcg agggagcctc
     2881 gtccattggc gttccgctca gcaccactca cgtgatttct accgccatca tgggcgttgg
     2941 cgcctccaaa cgggtttctg cggtccgttg ggccttggct aaaaacatcg tttgggcctg
     3001 ggttttaacc ctgccgatca ctgctttgct tggagcagga attgccatga tctttaaatt
     3061 gatcggttaa ttttttaagg gaacaggcac taggcaaaag gcaacagcgt tcttttttct
     3121 tatcgagttt ggtctgtcca agtctaaaat caaacacccc tcttgcctgt tcccttttgc
     3181 ctgttgcctc gcgccccagg gggcgcgctg cctcttgcct atatcctaaa ccgattcacc
     3241 gtttgcaaca gttggtcgcc ccaacgcgcc aattcattgc tggtgctgat gatctcttgt
     3301 gaacagctga aatgttcccc cgccgccgag gaagcctgtt cagttccttc tttatggtgc
     3361 aaggctatct ccgtgatcct gctcacagcc tgtagaatgt cctggtactt ctgtaccaac
     3421 tgacgggtat tgccggtaac ctcgccaagt gatctattaa ctctgccggt tgcgttactg
     3481 atcgattgga aaacctcgtt taaaaccgcg gtagcctgtt cactatcatg aatcatttgc
     3541 gtttcatctt ggatcatctg atttaggtcg ccaaacaact gttgaatccg ttccactaga
     3601 tcctgagtcg tttcggccgt agctttcgtt tgctccgaca atttcccgat tgatttggcg
     3661 acaactccaa agcctcttcc gaactcgccg gcccgtgccg cctcaatcga ggcgttcaac
     3721 gacaacagtg cggtctgttc ggcaatgttc tgaacagtat cgaccatatt ctcaatctcg
     3781 gttgataggg attgcagccg ctccatcgta tgttccgatt gagccataaa ccgttgaatc
     3841 cgttgaatct gccctccaac caccacagcg gcttcctcac ccttaactgc tttatccact
     3901 gtatcttcgg ccagccccgc ggcttttccc gtaatatcgt cgaccatttg cgaaaattgt
     3961 gtcgctgttt gaatcacttc ccggatggcg gccacttgct gctcttgcgt ttcagcccca
     4021 gccgccacct cggccatggt atccagcaac tgctgggcag cgccggtagt gatattggct
     4081 ccgtcctgca gccgatgact gctcaagctg acctgatcgg ccacccgctt gatctgttcc
     4141 gccaataacg caagctcatt cgacatcata ttaaaacagc ggcaaacctc cccaaattca
     4201 tccctgcggt cctccgggag tataaccttt aattcaccgg ccgcaatttt gcgggaggcg
     4261 gccgacatcc ggcgcaatgg cgtgataatc atccaggcag tcagaacact aattcctatg
     4321 accaatataa acgccactgc cagtaagctg aatgttgtat attgttgccg tagatagatc
     4381 cggttggctt ccacctgggc caaaaccgcc cgttgccggt aatagttctc attcatccgg
     4441 tcaatcccgc cgatcaggaa ataccgtaaa cggctaatct cggtttgaac ggccggttcc
     4501 agataactat ccggattttg cagcacacgc agatagattt tttcgtacag cttccaatag
     4561 tttctgatct cgccgagatt cgttgcttct tttcccggag cggctatttt tagtaattga
     4621 gtgaatgaat agtcgatgtt ttttgatatt gtattgggca gtaatgcatt tccgatatat
     4681 aataaagacg tccgatcctt ttgcagcatt gcattcttgg tttcaatctc aagctcggta
     4741 acgttggaac gaatatcatt caagtaaacc aatggttcca gttcactatg gatcaacgac
     4801 tcaagagttc cattcagctt ttgaatgccg ttctggctta cggccacaac caacaagaca
     4861 atggcccaga tgagcccgaa gctgatcaag agcttccaaa caatcttcaa atccaagtag
     4921 taacggatcg gccgataaag gatcttcttg agccaagcgc tatttttcat ttttaacgct
     4981 cctcattcat tcaatacctg atttagaaag actttcatat tacccgatga catccggtcg
     5041 gaccgactcc ttaacgaact gatgaattta tgagaacaga aaaaaaatca gccgatggac
     5101 tcgcctctta ttgaggcggg cctattaccg gcaaactcat tttcttgcca aaatccatgg
     5161 tgtgctgcgt atggcgtata ctgccaattg tttttcattt ctaaaaagct ctataaactc
     5221 ccttgacctt ttttagttga gatacttgtt tggttgtgta taaatcggcg ccgcatttct
     5281 tgcaatactc atcaaaactc aataccgtcg ccttgcaatc cggacaaatc attttttcac
     5341 ctcctctcca aatccttact gccaactcta aaataccatc ccacttttaa actaccattc
     5401 agttcagatt aagccggagt aaaattagat ctaaaaactc ggccggatat cacgaaggcc
     5461 ggcctatacg gccggccttc acataaatcc ggatttctat ttgatgcgaa tataatcctt
     5521 ggcaacaatt ttctgtcctt gaggaccagt aatccaatct aagaaagtct tggtcagacc
     5581 cgccggtttg gtcttggtca atagcaggaa tggtctttgc agcttgtagg ttttcgccaa
     5641 aacgttctta tctgtggcct ggataccgtc gatcgtcacc gctttaacgg atttggtatc
     5701 caaaaaaccg agtgagacat aaccaatggc ttctttggta accgccacag tttcctggac
     5761 cgccccggtg gaggcctgga ccagacaatt gctggtattc tccagcttgg ccggtttcag
     5821 cagcaattcc tcaaaagcgc cgcgggttcc ggaaccggct tcgcggttga cgacttggat
     5881 cttctgatcc gggccgccga cttccttcca gttggtgatc tggccgctat aaatcttctg
     5941 aagttgctcc gtagtgagat tattcacgga gttactcttg ctcacgatga ccgcaatacc
     6001 atcccgcgca attaccgtct cgacaatgcc ggtctgaagc tcggcggatt ccagttcccg
     6061 ggagcaagaa ccgatatccg cggtaccatc cacagctgct ttaattcctg cacctgagcc
     6121 tccgccctgg acgaaaacct tgacgccttt atttttagcc ataaacgcct gggctaactc
     6181 ttcggccagc ggctgaaccg aagtagagcc aacgatctga atggtgccgg agaacggagc
     6241 cgcaaaaccg aacgccgaca agcaaatgat acctgcgatt accaacgcga ttactttttt
     6301 catcacgaaa acctccttcg ttttttactg caattccaag ttatcatagg gcggttaagg
     6361 aatgattaaa gaaaagggca gtataaatta aggattgtta agaacaggtt tgaacctatt
     6421 ataaatcgat gattaatccg gatttccact tatctccctt tctccaatcg agagcggttt
     6481 attatcgggc tcttagcccg ttatcaccga cgaacttcac ctcaaatcca tcagcttcaa
     6541 ctcgccgcat ttcaaccagc cgatcgccgg attgtttata tgcttgtcgc aattctaaag
     6601 gttgccgacg gcctggacca tacccgtcaa tccaacgtct ccagtgtgca atgcatcata
     6661 gaaccgatac agattaagct cctctgccac ccccggcatt ccggcagtga agaagatctt
     6721 caatccgccc gtcaaaaagg aggatttttc gaaaaagttt atcaccggaa acttatgatc
     6781 gaatgggaac ttgcttgatt tttcttaatc tttacttaat atcaaccgaa tgttttcgca
     6841 aagatattat gttatgctta agaataagaa acaaaaaagc agtgatccta atttaacgag
     6901 aagggattat atggagttaa cactaaacat cattaagaaa gatccgttaa taaccgcttt
     6961 tattcaaaag gcggatgaac atcttgcggc aatgggcttt accgagcatg gattccgtca
     7021 tgtcagcctg gtatcgagca tctcccagaa cattttactc cgcctcggtt atccggacca
     7081 tcaagccgaa ttagccgcca ttgccggtta tctgcatgat atcggcaata ccgtcaaccg
     7141 taaggatcac ggccgcaccg gagcactaat ggcgctcaat tacttactaa gcaatacgat
     7201 ggatccgtcg gatgcggcta ctattgccgg agccatcggc aatcatgagg aagaatacgg
     7261 agaagccgtc aatgttatcg ccgcagcagt catcttagcc gataaatcgg atgtccaccg
     7321 cagccgggtc cgcaatgccg atctcgcaac ctttgatatt cacgatcggg taaattatgc
     7381 ggcggttcat tctttcctca acgtcaattc agaaaaaaag agcataactt tggaattgac
     7441 catcgaaacc gccatctgtc cggtgatgga gtatttcgaa atattcctcg ttcgaatgat
     7501 gatgtgccgc cgcgccgccg tttttctaaa ctgcaacttt aaaatcgaga tcaatggtgc
     7561 ccagatacta taaatcccag cccaataccg gccgggaaga cggggttttt gcatatttaa
     7621 cccaatgtta atcaaaactg cgtctttact taaacaggtc gtggtatgat tattttcgac
     7681 ggtagcgata ccggtggcaa cactaaaccg aaagtcccct gaagccaaag ggattgccaa
     7741 ataggcggct aggtttatca aagaaaagcc tgtaaagact ttcaacaatg aaagtctttt
     7801 ttatttccta ttttgcctcc agcctaacct caggatactt gaagatcatt tgatcgatta
     7861 acggttcttc cttacctttc aggtatttat tgaaaaaggc cgtcacataa tcattgacaa
     7921 tctgtgaatt cctttctccg ccaatcttgc cgaacattcc gatataccgg aaaaccggaa
     7981 aaagaatgct gaaatccata aaatcggaat gagtggcgcc cttgatgtat accacaaggt
     8041 aattggtctg ctccggagaa tagcctgctg tcaacgggat ttctctcccg tccgcggtga
     8101 tgagcatgaa cggttgcttg accggattgt tgataatgtc tccgaacgga ctgccgtcca
     8161 aattaataaa cgccttgaac cggaaatccg taatacaaac ttgacccgct gtggcgccgc
     8221 cgaatgaatg gccgaatact cccatgttcg ctacatcaag cctgccctca aagatgcttt
     8281 tgatctctcc actagcgagc ttctcaattt gatccaccac aaaactggtg tcgtcgctcc
     8341 atatatgtac gctttgatat gccattttgg attgttcgat tccatacttc acaggattct
     8401 caggcttctt ggcttttgtt tccttcgccg ctctcaaaag atcatcagtg aagaccttca
     8461 tttgtttggg atcaaaatag gcaattcttc catccgggaa tatactggcc acatcctcat
     8521 aggtatggga gatactgaat accacatagc catgactggc cagttcctcc atttgaacgg
     8581 tatttgcgtt cccgatgttt ccgtagcctg cggaaaatac aaccaccgga tacttatccg
     8641 ttccggcgga cagtcccgcg tccaggtagc tgttagttct gactgcggca agtttgccaa
     8701 atacattcgg cattcccaaa cgtttggcta tatacttacc gactgtccga tcgggaatat
     8761 aaaataatgg ctccttgcct tggagatcat ccgttgtcgg ataccaaacc tgtaccgcaa
     8821 tctcccgata ttcgtccgga ttggatgtga agatcccctt tcttgatgga tcgacaaaac
     8881 ccaggcgcat ggttcctacg gaattcgggc cgctaggttg gggcagatca aaatttcggg
     8941 ccaataccgg aaatgtcaat gaagctagca gtaccattat aaaaaacgcc ttcagaaaaa
     9001 ctttcatatg aatcgcctct cgcattctcg taaagttgct tgcctgataa tcatattaac
     9061 agggcaatct ttacggcgga tcaatctatt cttaatgcaa ccttaaatct gcgagaggca
     9121 gaccaattgt gaatatagtt accgcttctt cagcggacga caggataatt gtgccgttat
     9181 gcctttggat aattttcttt gcgatggcaa gccccaagcc cgtgccgtct ttggcgcttc
     9241 cctcctcgtc gccgcggacg aaggggtcaa aaataatatc ccgcaattcc gcgggtatcc
     9301 cgatcccatt gtctgctatt tggagtacgg cttgactctc ctcccggcgg cagcggatcc
     9361 gaaccctggt ctgttccgga ttatgcttga gaacattgta taacagatta ttgacggccc
     9421 ttgtcatctg aatgggatca aaatcaacca taataggttt ttcttcgata tcaatatcaa
     9481 gaataaattt cttccgttca aattcgggat agatctctcc gaccaattgc ctcagccatt
     9541 ccgccagatt attacggctg tatgcaaact gaaagttttc atcctcaagc ttggaaagtt
     9601 caaataagtc atcaaccaag ccggcaacat aatgggcttt attatagata tatgtgacat
     9661 acttctcccg gtccttggcg gcatcgatct ccccatcatg gagtaatttc gaaaaaccgc
     9721 agatcgatgt gataggtgtt ttcaagtcat gcgatatatc gaccaacatc cgttgtttgc
     9781 tctcttccag cctccttttt tcggactcgg ctttttccag cctggtgatc atctcgttaa
     9841 atgcatccct gatccaggca aattcccttt ccgcataaaa atttaaccgg aaccggtaat
     9901 tttgtttctc catttctttg atgccatcca tgagatagcc gagaggcttc cgaatatgtt
     9961 tggccagaaa tttgctgtaa atatacatgc cggccaggaa tagaattaaa aaacagccgg
    10021 atatggctac ccacgctatg atgtgcaacc ggttgggatg ggaaaagact gcccagaaat
    10081 ttgcggtgat ggagacatga tacaatgact tggggagctt tatgataaat aaataatcct
    10141 tgccagctgg gcctttaaca ggatatatat tgtaaataaa cggatatctg cccgtatctc
    10201 tgtgaagtat cgccgcatca tcaaacaact ggttttgatc atattgatag atctgatccc
    10261 gcttagttcc cctcacaaaa ataacccgac ggttttcatc caatatttcg atccatccgc
    10321 cgactgcttc aatgcttttg gtattcatcc tgtcaaaggg atatgtatac gttttttccg
    10381 atccgatgta ttcccaattt aaatacttga atatcaagat ctccgaaatc gtgttacaaa
    10441 taaacctggc aataaaaaaa ataaccaggg caatcagaac gataactacg gaatttctaa
    10501 acaaaaaacg tccgatagat tttttgtctt tcattgaatc tccccttcaa atctatagcc
    10561 caggccccgg atggtcttta aatatcgcgg gtttttgggg tcaccctcaa tcttctccct
    10621 aagcttactg atataaacca taataatatt ttcgtcgccc ataaactctt ggccccatac
    10681 cctctcgtac agctgttgct ttgtaaagac ccggttgggg ttggttatca aaaaaagcaa
    10741 cagtttatac tccatggagg tcagttccac ttttgcatca tttttatata attcgcaact
    10801 gtcctgccgc aggatccaat ccccgaattt aatacattcc ccagcctggt ta
//
LOCUS       DASVMB010000476         4842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_354741,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000476 DASVMB010000000
VERSION     DASVMB010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354741"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VF531_13370"
     CDS             complement(<1..377)
                     /locus_tag="VF531_13370"
                     /inference="COORDINATES: protein motif:HMM:NF020902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HEX7714997.1"
                     /translation="MLKNYAPSSLNRDDAGSPKSCIFGGVARGRISSQCLKHHLRESP
                     LLAEAVKASLSGMRTRKLPQLVASELSGRGIPPEWVAASVRKVTGFGNKDGKENETDL
                     TAQLIFVTPEEVKLAADCIQGVI"
     gene            complement(421..921)
                     /locus_tag="VF531_13375"
     CDS             complement(421..921)
                     /locus_tag="VF531_13375"
                     /inference="COORDINATES: protein motif:HMM:NF021034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HEX7714998.1"
                     /translation="MPIKKFINNLESLSGRERTILRHNAESSIVPTGGDALTIFYKVL
                     PYKIAPDEEELWFVAAMIRFYSRCSIPGIHEANNNDFGWTLRKIRNGDSFDQRVRALL
                     DCRYQKGDRVLADRLRHMVKLAEIKQVGINWPVFTMDLLHWEDPERSTQKKWARSYYG
                     GQTGVF"
     gene            complement(896..2497)
                     /gene="casA"
                     /locus_tag="VF531_13380"
     CDS             complement(896..2497)
                     /gene="casA"
                     /locus_tag="VF531_13380"
                     /inference="COORDINATES: protein motif:HMM:NF021030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HEX7714999.1"
                     /translation="MASFNVLTEPWIPVMELNGTIRETGIIQLLAQAPGLKEIYEPSP
                     LMKYGIHRLLTAFLTDALRPERDADLSELASLGCFPMERILEYVAECEKDGPCFDLFD
                     RERPFLQCPYSESLDSSKMTSVAKLLHHLPSGNNAVHFEHSFQDEQAFSAAICTRALT
                     AVNVFCTSGLQGPSGMNGAPPWYLIIVGETLFDTLLYNLWLPGEGMPFNRPAVAWRNH
                     SEVIPNHEIEHTSYLYGLTWPARRVCLVPGTGEGRCTYTGIQSDRIVKDIYFQAGLKF
                     VGHHQWRDPFVAYLITREGRVSLKPRENREAWRDLGALLFNHSGVLGDEVIQTEPPAI
                     IEQYHRMIRNGLWERQTIFRIELFGLVTDKAAYEEWLHDRFAIDPRVAADMQKSAWLG
                     GALGQAEQINKSLRRALQRIQPQHGKVKAIQKRCCADLIDQAEFQFFTAIRIRFFEEL
                     LPRVADTDISEWDRQQELDRFWRGVLALEAQAVLDRIVEGIGHSARNLERGVIAEKVL
                     VGDLDRLLKGGDGDNRGDAYQEVYQ"
     gene            complement(2776..3204)
                     /gene="fucU"
                     /locus_tag="VF531_13385"
     CDS             complement(2776..3204)
                     /gene="fucU"
                     /locus_tag="VF531_13385"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HEX7715000.1"
                     /translation="MLKGIPKVISPELIKIMLEMGHGDELVLGDGNFPAVSCAQRVVR
                     ADGLNIPVLLDAILRFYPLDKYVKAPVALMAVVPGDTVQPTIWEEYRRIVAKYDSQAA
                     GFEFVERFAFYERAKKAFAIVATGEEALYANVILRKGVVA"
     gene            complement(3268..4188)
                     /locus_tag="VF531_13390"
     CDS             complement(3268..4188)
                     /locus_tag="VF531_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715001.1"
                     /translation="MCVAALLGGLALAADNSRIYIPVISKGFQHQFWQAVKLGAEKAA
                     KEFDVDITFEGPETEAMVDKQIDMLQTALNKKPKAICLAALDSKACIPLLEKAKTANI
                     PVIGFDSGVDSNIPVATAATNNVAAAALAADKMAQLIGGSGEIAVIVHDQTSRTGIDR
                     RDGFVNRIKSAYKNITIVDLQYGGGDQLKTTDLAKAIMQAHPKLKGFFGANEGSAIGV
                     INAVNEMKKAGKIVVIGYDSGKLQLDAIRSGLMAGAITQNPVGIGYEAVKAAVMEIKG
                     QPIKKNIDTGFYWYDKSNIDNPEIKPNLYD"
     gene            complement(4301..>4842)
                     /locus_tag="VF531_13395"
     CDS             complement(4301..>4842)
                     /locus_tag="VF531_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715002.1"
                     /translation="SLVISGLKPIYFVDTPDFYKISMSTLWGIPNAVLLFFGAAIIAS
                     LVLTKTVLGRYTFALGSNEEAARLSGIKVNSWKTAVYILGGVFSGLAGIMMASRINSA
                     QPALGSGYEMQAIAAAVIGGTSLSGGEGTILGTVIGAFIMSVLTNGLRILSIPQEWQT
                     VIIGAIVILAVYLDIIRRR"
BASE COUNT         1205 a         1350 c         1092 g         1195 t
ORIGIN      
        1 tttattacgc cttgaataca gtccgcagcc agcttcactt cctccggagt gacgaagatc
       61 agctgggccg tgagatcagt ctcattttcc ttcccatcct tgttgccgaa gcccgttacc
      121 ttcctgacgg acgcagcgac ccattccggt gggatccccc tgccgctcaa ctcggaagcg
      181 acaagttgcg gtaatttccg ggtgcgcatg ccggaaaggg atgctttcac cgcctccgcg
      241 agcagcgggg actcacgaag atgatgtttg aggcattggc ttgatatccg gcccctggcc
      301 accccgccaa atatacaact cttgggactt ccggcatcat cccgatttaa acttgatggg
      361 gcgtaattct ttaacatatg aacttccatt aacatattgg ataatccctc ttgaaaagta
      421 ttaaaagact cctgtttgac ccccataata cgagcgagcc cacttctttt gggtagaccg
      481 ttccggatcc tcccaatgca aaaggtccat agtgaaaacc ggccagttaa tccctacctg
      541 cttgatttcg gcaagcttca ccatatgtcg cagcctatcc gccagaaccc tgtcgccttt
      601 ttgataccta cagtccagta aggcgcgaac ccgttgatcg aaactatccc cgtttcgaat
      661 cttgcgaagt gtccaaccaa aatcgttgtt gtttgcttca tgaataccag ggatggaaca
      721 acggctgtaa aaccggatca tagctgcaac gaaccagagt tcctcctcat ccggagcgat
      781 cttgtagggc aaaaccttat aaaatatagt tagggcatca ccgccggttg gcacgataga
      841 ggactcggcg ttgtgcctga gtatcgtccg ttctctgccg gacaaagact caagattatt
      901 gataaacttc ttgataggca tctccccggt tatctccatc tccacctttc aacaacctgt
      961 ccaaatcccc aaccagaacc ttttccgcaa taacgcccct ttccagattc cgggcactat
     1021 ggccgattcc ctccacaatc cggtccaaga ctgcctgggc ctccaaagca agtactccac
     1081 gccagaatcg gtccaactcc tgctgtcggt cccactcgga gatatctgta tctgcaaccc
     1141 ggggaagcaa ctcctcaaag aaacggatcc ggattgcggt aaagaactga aattccgctt
     1201 gatcgatcag atccgcgcaa caacgtttct gaatcgcctt gacctttcca tgctgaggct
     1261 ggatccgttg caaggcccgg cgcagacttt tgttgatttg ctctgcctgc cctaacgccc
     1321 cgcccaacca agcggatttt tgcatatccg ctgccaccct ggggtcgatc gcaaaccggt
     1381 catgcaacca ctcctcgtaa gcagctttgt ccgtaactaa accgaagagc tcgatcctga
     1441 atattgtttg ccgttcccaa aggccatttc gaatcatccg atgatactgt tcgatgatcg
     1501 ccggcggctc ggtttggatc acctcatctc ccaaaacccc gctgtggttg aagagcaagg
     1561 ctcccaaatc gcgccaggcc tctctgttct cccggggttt cagggagacc cttccttccc
     1621 tggtgatgag ataggccaca aatgggtccc gccattggtg atgaccgaca aatttcagtc
     1681 ccgcctggaa gtagatatcc ttcacgattc gatccgattg aatccctgta taggtgcaac
     1741 gtccctcgcc ggttccggga accaggcaaa cccgtcgcgc cggccaggtc aaaccgtaca
     1801 aataggaggt atgctctatc tcgtgatttg gtatgacttc ggaatggtta cgccaggcta
     1861 ccgccggacg attaaacggc atcccctccc cgggcagcca aagattataa agtaacgtat
     1921 cgaagagggt ttcccccacg attatcaggt accaaggtgg cgctccattc atacccgaag
     1981 gtccctggag tccgctggtg caaaaaacgt tcacagcggt taaagccctg gtgcagatcg
     2041 cagccgaaaa agcctgttca tcctggaaag agtgttcaaa atgcaccgca ttattgccgc
     2101 tgggcaggtg atgcaggagc ttggcaactg aggtcatttt cgaactatcc agggattcag
     2161 agtacggaca ctgtaaaaat gggcgttccc tatcaaaaag atcaaaacag gggccgtcct
     2221 tttcacattc tgccacatat tccaggatac gctccatagg aaagcaccct aaagaagcta
     2281 gttcggatag atcggcatcc cgctccggcc gaagcgcatc agtcagaaag gcggtcaata
     2341 atcgatggat cccgtacttc atcagtggcg agggttcgta gatctctttc aaccccggag
     2401 cttgtgctaa aagttggatg attcccgtct cccggatggt cccgttcagt tccatgacag
     2461 gaatccaggg ttcggtcaat acattaaaag acgccatccg ttccatcccc tgaagctata
     2521 attgctgtgg gttattatta atattacgat attttcattc caactcctcc aacttttcgt
     2581 aataaacata cacttcttat ataggacaat aaactaatac ttcttatatc agggaattct
     2641 cttggattca aaataacacg gattaaaaaa agaaacactc accaaatgga gtgtttattt
     2701 ttttcaatat agtagatgtt gtctggaaga gggggaatta aaacagaccc ttttgcctct
     2761 ttctcttttt aatttctatg ccaccactcc cttcctcaat atcacattgg catacaacgc
     2821 ctcttccccc gttgccacaa tggcgaaagc ttttttggcc cgctcgtaaa aggcgaaacg
     2881 ttccacgaat tcaaaaccgg cggcttgcga atcgtatttc gccacaatcc ggcgatactc
     2941 ttcccaaatg gtgggctgta ccgtatctcc gggcactacc gccatcaagg ccaccggggc
     3001 cttcacatac ttatccaaag gataaaaacg caaaatcgca tccagcagca ccgggatatt
     3061 taacccgtcg gcccggacta ctctttgggc gcaagagaca gccggaaaat taccgtcacc
     3121 aagcaccaat tcatccccat gtcccatttc cagcatgatt ttgatcaact ccggtgaaat
     3181 cactttgggt atacctttca gcatcggata catcctttcg taaaaaggga ggcctcaagg
     3241 cctccctact gtattcggcg aaaaatctta atcatagagg ttcggtttaa tctccggatt
     3301 atcgatattg ctcttatcat accagtaaaa tccggtatcg atgttctttt taatcggctg
     3361 acctttgatc tccatgacag cggccttcac agcttcataa ccgatgccga ccggattttg
     3421 ggtaatcgca ccggccatca atccggagcg gatggcgtcc aattgcagtt taccggaatc
     3481 ataaccgatg acaacgatct tgccggcttt tttcatttcg ttcaccgcat tgatcacgcc
     3541 gatggcggaa ccttcattgg cgccgaagaa accttttaac ttgggatggg cttgcatgat
     3601 tgccttggca agatcggtgg ttttgagctg atcgccgccg ccgtattgca gatcaacgat
     3661 ggtaatattc ttataggctg atttgatccg attgacgaaa ccatcgcggc ggtcaatacc
     3721 ggtacgactg gtctgatcat ggacgataac ggcaatctca cctgaaccgc cgatcagttg
     3781 ggccatttta tctgctgcca acgctgcggc ggctacgttg ttggtggcgg cggtcgctac
     3841 cggaatattg ctgtccactc cggagtcaaa tccaatcacc gggatattgg cggttttcgc
     3901 cttttcgagc agaggaatac atgccttact gtccaaggca gccaggcaaa tggctttggg
     3961 cttcttgttc agggcggtct ggagcatatc gatctgctta tcaaccatgg cctcggtttc
     4021 cggtccttca aaggtaatgt ccacatcaaa ctctttggca gccttttccg ctcccaattt
     4081 aaccgcctgc cagaactgat gctggaatcc cttggatatc accgggatgt agatcctgga
     4141 gttgtccgcc gccagggcca gaccacctaa aagggccgct acacacaata aggttaccaa
     4201 gataaagatc ttttgggtct tcgtcactac ctattcctcc cctttattta ttattaagcc
     4261 ccctcccgga gcttaaaatg gtcccataca agcatcgctt ttagcgacgg cggatgatat
     4321 ccagatatac ggctaatata acaatggcgc caatgatcac tgtctgccat tcctgcggta
     4381 tggaaaggat gcgcaaccca ttggtcagca cactcatgat gaaagcaccg ataactgtcc
     4441 caagtatagt gccctccccg ccgctaagag acgtacctcc gataaccgcc gcggcaatgg
     4501 cctgcatctc ataacccgaa cccaatgccg gctgagcgga gttgatccgg gatgccatca
     4561 tgataccggc caggccgcta aagacaccac ccagaatgta aaccgcagtc ttccagctgt
     4621 tcactttgat accggagaga cgggccgcct cctcgttact accgagcgca aaggtatatc
     4681 tgcccagcac cgtcttggtc aagaccaagc tggcaatgat ggcggcgcca aaaaagagca
     4741 gaactgcatt gggtatgccc catagggtac tcatcgaaat tttgtagaaa tcgggcgtat
     4801 caacgaagta aatgggtttt aaaccggaga taaccaggga aa
//
LOCUS       DASVMB010000477         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_499149,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000477 DASVMB010000000
VERSION     DASVMB010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499149"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..863
                     /locus_tag="VF531_13400"
     CDS             525..863
                     /locus_tag="VF531_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX7715003.1"
                     /translation="MPSKKMDEVVDGIYVEPTPVTLGEEVKIKYKGLLAGSGAGKVFL
                     HAGYGVGEWEKVMDIPMRKTRDGGWSVTVQVEEPSSFNFCFRDDAQNWDNNFGKNWVY
                     QIHTGEIVGH"
     gene            992..1342
                     /locus_tag="VF531_13405"
     CDS             992..1342
                     /locus_tag="VF531_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715004.1"
                     /translation="MSILVVDDSPLVRAVLSDYLREAGFEVYEAGTCREAELQFQQHH
                     SGLVLKDLYMPEWDAIESIRFFRNLDPQVKVVICSTDSSKATILEGLKAGAQDFLLKP
                     FDKDQVLSLIKRLA"
     gene            1502..2224
                     /locus_tag="VF531_13410"
     CDS             1502..2224
                     /locus_tag="VF531_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715005.1"
                     /translation="MFKKILVFGTLIIIFIALMTPSGADPTDVALDQQVELGVPGTFE
                     LVDNNQDQKAEAIQFKLTLKAYREGNFIVTGNLEGNRNGDWVALATTVIPFQWSPDHN
                     QLELKFLAGNILKYKISGPYRVVLSLKEGDWELPAQVVGFSPKYSLSTFSNATTVNSG
                     SITTISQAQRAAETWADFQNISLGELKQVNYNYDRWELEYRGRSGEALRFLITPQGKV
                     ELMRIKNPTANGRSFSGSYPAK"
     gene            complement(2209..2913)
                     /locus_tag="VF531_13415"
     CDS             complement(2209..2913)
                     /locus_tag="VF531_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX7715006.1"
                     /translation="MLQKRWIGAVLAVSFGLGSILNPLLISGAANQNGFSGVNATQNG
                     VTVAPESIQTPIKTHTVGQKDRTLVSKPNENVAVNSTAPNQTGKSRSAGIMNQSGTGT
                     NNAHRAASNTRTGLSNNQFQILVRVIKAEAGGEPFLGQVAVGAVLMNRIHSGRFPKTI
                     SANVFRNNEFESVSNGYIWDIEPTADSYRAATLAVKGWDPTGGALYFYNPAKSSSAWI
                     WSRPIYTKIGDHYFAG"
     gene            3235..>3389
                     /locus_tag="VF531_13420"
     CDS             3235..>3389
                     /locus_tag="VF531_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7715007.1"
                     /translation="MDLFSATADERREKESPLAARMRPRSLDQFVGQEEILGEGRLLR
                     RAIEADRL"
BASE COUNT          863 a          741 c          866 g          919 t
ORIGIN      
        1 gacgaagtgg tagaacggga tgattgtttg ggctcggctg tttgacccgg atactgtttc
       61 cgaaaataac aagttgcttt tgttttattc aagtgtaaag caattcagta ttagttaacg
      121 cccaaaatca acaggattaa aatcaggaaa accacaaatc caaggttatt cccgaggaaa
      181 ccaacgttct ctttacagca ttttttgtct gacattattc accattcctt tccatcgata
      241 tgggttgatc tttgaatatc ggtgtcgtac ctcatattgc atcgcggtca gttatccata
      301 ataattggcc atccggtact ggtataagat atgaatcgct ctgagcagtt ggaatagcct
      361 attagtataa attgcccggg accttggatt gaaccgtatt cccggaacga tgggctgtat
      421 gtcaggtctt ggaagtctgt ttaacccgcc acctccgggg cataataact caagaagctt
      481 gcccggaggc aagtcggagg agtttacgat gggaggaata ggtaatgccc agtaaaaaaa
      541 tggatgaagt ggtcgacggt atttatgtgg aaccgactcc ggtgactttg ggggaggaag
      601 ttaagattaa gtataaggga ttattagccg gttccggggc tggtaaggta tttttgcacg
      661 ccggatacgg cgtgggggaa tgggagaaag tgatggatat cccgatgcgg aagacccggg
      721 atgggggatg gtccgtaacg gtccaggttg aagagcctag cagcttcaat ttttgtttcc
      781 gtgacgacgc ccaaaattgg gataacaact tcggaaaaaa ctgggtctat cagattcata
      841 ccggtgaaat tgtaggacat tagaaaatta ttatttcggg aaaaaggaaa ataacggaat
      901 aagacgaaat tctatatctg aatttccgac ataaatgatt tgtgaaacct cttcccgatt
      961 tgagtcaatc tcaactctaa agggggatgg aatgagcatt ctagtggttg atgattcacc
     1021 cttagtgcgg gcggtattaa gcgattattt gagggaagcc ggttttgagg tttatgaggc
     1081 gggtacctgc cgggaagcgg agctccaatt ccagcaacat cattccgggc tagttttaaa
     1141 agatctttac atgcctgaat gggatgccat tgaatccatc cgcttttttc gaaaccttga
     1201 tccccaggtg aaggtggtta tttgcagtac cgattcttca aaggcaacaa ttcttgaggg
     1261 gttaaaggcc ggagcccaag attttctgct taaacctttt gacaaggatc aagtgctttc
     1321 actgatcaaa cgcttggcat agttggcgaa tcttacataa aggattgtca gttttttaaa
     1381 cgaaatactc cgcaaacttt tacagcaaga tgatagatta accgaccatt aattcggatt
     1441 gtttaggtac ctgccgaatc acggttagtt gcagcttgtt gtatgatgcg gagtgatttt
     1501 tttgttcaaa aaaattctgg tttttggaac cctcatcatc atcttcattg cgctgatgac
     1561 tcccagcggc gccgacccta ccgacgtagc cctggaccag caggtggagc tgggggtacc
     1621 gggtaccttc gaattagtcg ataataatca ggatcaaaag gcggaagcaa ttcaatttaa
     1681 actgacgctc aaagcatacc gcgaggggaa cttcattgtc accggcaacc tggaagggaa
     1741 ccggaacggt gactgggtgg ctctcgccac cactgtgatc cctttccagt ggtcgccgga
     1801 ccacaatcag ctggagctga aatttctagc tgggaatatt ctcaagtaca agatatccgg
     1861 tccctaccgg gtggtcctta gtttaaagga aggagactgg gaactcccgg cccaggtagt
     1921 cggcttttct ccaaagtatt cgctttcaac ctttagtaac gctaccacgg tgaattccgg
     1981 atctatcacc accatctccc aggcccagcg ggcagcggaa acctgggccg attttcagaa
     2041 tatcagcctg ggtgaactga agcaggttaa ttataattat gatcgctggg aattggagta
     2101 tcggggccgt tccggagaag ccctccgttt tttgattaca ccccagggca aagtggagct
     2161 gatgcggatc aaaaacccta ctgccaatgg caggagcttc agtggctcct acccggcaaa
     2221 gtaatggtct ccgatcttgg tgtaaatcgg acggctccag atccaagccg aactgctttt
     2281 ggcggggtta taaaagtaca acgcgccccc ggtgggatcc cagcctttta ccgccagcgt
     2341 ggccgcacgg tatgagtcgg cggtcggttc gatatcccag atatagccgt tggataccga
     2401 ttcgaactca ttgttccgga atacattggc cgatatggtc ttggggaatc tacccgagtg
     2461 gattcggttc atgagaaccg cgcctaccgc cacctggcca agaaaaggtt ccccacccgc
     2521 ttcggccttg atgacccgga ccaggatctg aaattgattg ttgcttaaac cggttctagt
     2581 gttgcttgcc gctcgatgtg cattatttgt tccggtaccg gactgattca ttatcccggc
     2641 agaccgtgat ttcccggttt gattcggagc cgttgagttc acggcaacat tttcattcgg
     2701 ttttgaaaca agggttcggt ctttttgccc cacagtgtgt gtctttattg gggtctgaat
     2761 cgattccggc gctacagtta cgccgttctg cgttgcattc actccgctaa atccattctg
     2821 gttggctgcc ccactaatca ataagggatt aaggatggaa cctaacccga aactgaccgc
     2881 aagtaccgcc ccgatccaac gtttttgtaa catcgaaatc acctcttctt cgttaatcta
     2941 ctttcttttt ccattttttt ctgctgtttg acagaaacaa atagtattat aacagcttct
     3001 cctgggaaga tcgagaggaa ataattgtct aatccgcacc cggattgatt ttttggttta
     3061 tggcacttga ggattgagca aggggaaggt ttcggttttt gagccggggg tgagtcgtct
     3121 ttgttaagca atcataacaa ctattaactg ctgtaaagca ggattctgca gtttttggta
     3181 aaagataaac aagatggttt cggtttgatc caagatgaat ggagtgaatt tgggatggac
     3241 ttgttttctg caaccgccga cgaacggcgg gagaaagaat cacctttggc agcaagaatg
     3301 cgtccccgca gcctggatca gtttgtcggc caggaggaaa tcctaggtga aggccgcctg
     3361 ttacgacggg ctatcgaggc ggaccggct
//
LOCUS       DASVMB010000478         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_428381,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000478 DASVMB010000000
VERSION     DASVMB010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428381"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF531_13425"
     CDS             <1..258
                     /locus_tag="VF531_13425"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEX7715008.1"
                     /translation="VFERVSGQGKAVETPIGYMPSMDAIDRGGLNVSDAEMAELLKVD
                     RDEWLKEAASIREYYTKFGDKLPQELRKEIDALEERLRDAK"
     gene            372..1760
                     /locus_tag="VF531_13430"
     CDS             372..1760
                     /locus_tag="VF531_13430"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX7715009.1"
                     /translation="MQVKITETVLRDANQSLIATRMPFADFEGILEELDQAGYYSLEC
                     WGGATFDSCLRYLNEDPWERLRLMKAKVKKTKLQMLLRGQNLLGYKHYPDDVVRIFVR
                     LAVESGIDIIRIFDALNDFRNIEVAVDEALKNKAHAQGTIAYTLSPIHSLEKYAEMGK
                     ELERMGVNSICIKDMAGIMGPQEAYELVARLKAAVKIPVFLHTHSTTGLGPMTCLKAI
                     EAGCDGIDTAISAFSGGTSQPATETMHYALKQMGRQTGLNEKTLKRVNDFFKPLRAKY
                     IESGLLNPLVLGTETDALVYQIPGGMLSNLIAQLKQQNSLDKLEEVLAETPRVREDLG
                     YPPLVTPMSQMVGTQAVANVLAGERYKIVIKELKAYLKGEYGKPPGPVNPDLLHKVLG
                     AEQPITTRYAASLEPVFEKTQKQTGSFAKTDRDVISYLIFPQIAEAFLTCKQTAASSA
                     ERKFSYTIECRG"
     gene            1805..2134
                     /locus_tag="VF531_13435"
     CDS             1805..2134
                     /locus_tag="VF531_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HEX7715010.1"
                     /translation="MSIAEKLLLGLNTTVIGMGIVFLVLIVLSVIVGIIGKLFMAKSK
                     GTPVAGPDLPPTAGIRSQADKSGVTAGEVTAVGADEETAAMIMAALSCHLDIPLGKLK
                     IRSIKAL"
     gene            2173..2517
                     /locus_tag="VF531_13440"
     CDS             2173..2517
                     /locus_tag="VF531_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7715011.1"
                     /translation="MKYWVTVNGKKFEVDVEQAPGGQTVNPGENSSAAVQPGSVKSGD
                     GDLILAPMPGTILEVKVGEGHAVKKGDVLFILEAMQMENEIMAGRDGSVARITVARGA
                     SVKTGDCLAVLK"
BASE COUNT          659 a          625 c          774 g          633 t
ORIGIN      
        1 gtgtttgaac gggtatcggg tcaaggtaag gccgtggaaa caccgattgg ttacatgcct
       61 tccatggacg ccattgatcg cggcggcttg aatgtcagcg acgccgaaat ggccgaattg
      121 ctcaaggtgg atcgggatga atggttgaaa gaagcggcct ccatccgcga gtattatacc
      181 aaattcggcg ataagttgcc gcaagagctc cgtaaagaga tagacgctct agaagaacgg
      241 cttcgtgatg ccaagtgagc ttggaaccgt acggggatta tccgggcgcg ggcggagtta
      301 gtcccgcccc gtccggaaac agatagagca tttacttgac gcgtgcacag ttaacaaagg
      361 agttggacta tttgcaagta aagatcaccg aaaccgtgct tcgcgatgcc aatcagtccc
      421 tgattgccac cagaatgccg tttgcggatt ttgaagggat tttggaagaa ctcgaccagg
      481 ccggctatta ctcgctagaa tgctggggag gggcgacctt tgactcctgt ttacgctatt
      541 tgaacgaaga tccctgggaa agattgcgct tgatgaaggc gaaggttaaa aagaccaagc
      601 ttcagatgtt attgcgcggc cagaacctgc tgggatacaa gcattacccc gacgacgtgg
      661 tgcggatctt cgtcaggttg gcagtggaat cggggattga tatcatccga atcttcgacg
      721 cgctgaacga tttccggaat atcgaggttg ccgtggacga ggccctcaaa aacaaggccc
      781 acgcccaggg aaccatcgcc tacaccctga gccccattca tagtttggag aagtacgccg
      841 agatgggcaa ggaactggag cggatggggg ttaattccat ctgtattaag gatatggccg
      901 ggatcatggg cccccaggaa gcctatgagc tggtcgccag gttgaaggcc gccgtcaaga
      961 ttccggtctt cctccatacc cattccacca ccgggctggg gccgatgacg tgtttgaaag
     1021 ctatcgaagc cgggtgcgac gggatcgata ccgctatctc cgctttttcg ggcgggacat
     1081 cgcaaccggc caccgagacg atgcattatg ccttaaaaca gatggggcgc caaaccggcc
     1141 tcaatgaaaa aacgcttaag cgggtcaatg atttctttaa acccctcaga gccaagtata
     1201 ttgagtcggg actcctcaat cccctggtac tcgggactga gactgacgct ttggtttacc
     1261 agatccccgg cggcatgctt tccaatttaa ttgctcagct aaaacagcaa aattctctgg
     1321 ataagttgga agaggttttg gccgagaccc cgcgggttag agaggatctg ggttatccgc
     1381 cgctggtgac cccgatgagt cagatggtag ggactcaggc tgtagccaat gtccttgccg
     1441 gagaacgtta caagattgtc atcaaggaac ttaaggctta tcttaaaggg gagtacggca
     1501 aaccgccggg gccggtcaat cccgacctgc tccataaggt gctgggcgct gaacaaccca
     1561 ttactacccg ttacgcggct tcgctggaac cggtttttga gaagactcaa aaacagaccg
     1621 gctcgtttgc caagacggat cgcgacgtaa tctcgtattt gatcttcccc cagatcgccg
     1681 aggcgttcct cacctgtaag cagactgcgg cttcctctgc ggagcggaag ttttcttaca
     1741 ccattgaatg tcgcggttag tggttggttt gattgtcaga aacatctaaa aggtgggata
     1801 cagggtgtcc atcgctgaaa aattgttgct tgggttgaac acgacggtca tcggcatggg
     1861 aatcgttttc ttggtgctga tcgtgttaag cgtgattgtt ggaattatcg gtaaattgtt
     1921 tatggccaaa tcgaaaggta ctccggtagc cggtccggat ttgccgccca ctgccggaat
     1981 taggtctcag gcagataaat ctggagttac agcgggagaa gtgacggcag tcggagccga
     2041 tgaggagacc gctgcgatga ttatggcggc gcttagctgc cacctggata tacccctcgg
     2101 caaactaaag atccgttcca ttaaagcttt ataattttga aatcaggatg aaactgggag
     2161 gttcagtcca tcatgaaata ttgggttaca gtgaatggta aaaagtttga ggttgatgtg
     2221 gaacaagcgc cgggtggtca aacagttaat ccgggtgaaa attcttctgc cgctgtgcag
     2281 ccgggttctg tcaaatcggg cgatggtgac ctgatccttg ctccgatgcc cggaacgatt
     2341 ctggaggtta aggtgggtga gggacacgca gtcaagaaag gcgatgtttt gttcatcctg
     2401 gaagcgatgc agatggagaa tgagatcatg gccggacgcg acggttcagt agccaggatc
     2461 acggtcgcgc ggggcgcctc ggtaaagacc ggcgactgtc tggcagtgct taaataataa
     2521 tcgcgaaagg gttgccgcaa tgtcattttt tgatatggta ctcaaagcta tcgtcgacat
     2581 ctggaaaacc tcgggcttcg tcagtatgac ctggggacag atagtgatga tcgctgtttc
     2641 attcttacta ctctatctgg cgattaaaaa aggctttgaa ccgttactgt t
//
LOCUS       DASVMB010000479         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_428391,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000479 DASVMB010000000
VERSION     DASVMB010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428391"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF531_13445"
     CDS             complement(<1..256)
                     /locus_tag="VF531_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7715012.1"
                     /translation="MRIEITYIFHNSFIVRMGQEALLFDYPAAQFLDEVRRNIVRKLI
                     QDTDLYVFASHNHPDHFNRDIDELGGCTSRVSYILARDIMR"
     gene            449..1015
                     /locus_tag="VF531_13450"
     CDS             449..1015
                     /locus_tag="VF531_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7715013.1"
                     /translation="MRKIFLLLLSLLFTVFVLSGCSNLQEPAKPEDNLLIGRVTLQAQ
                     NFENFGNISINGSISKNIQVTLINTETGKRFNVTTADEGVFSKRNCEPGQYRIEKFLY
                     QANASDSQFRYTSNIYCNVQSEQIITISTGVVIDLGSLVWYCDGALNRSWFQPEGSPE
                     ALKDYFTQLFPESLWLEREWVMAQLTGK"
     gene            1145..2083
                     /locus_tag="VF531_13455"
     CDS             1145..2083
                     /locus_tag="VF531_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX7715014.1"
                     /translation="MTSDQEFIVQSLELNLFFMRIAKEHSFFIEVAIPSKNFDLIAQA
                     DSFKNRFAGLLTETTCLADGYLTPEFLNSGEMVTDLTLEAERGSEFYSGYTLNTEITL
                     AERTLSGGLSSCEATTVQLVGCLNAQAMTATRELIDFKARLLSDVLACQVFTFNYPLL
                     LDHILREARFYLCMLERIQCRQERNLVAEAVEQEIFWNRIMAEHAKFIRGLVDPTEEQ
                     LFTTADNFGKQFDGLTLQAQTLLTQTAGLPNVTEESRRATVAIRDFKRQGTEGLITCR
                     IRSIAHPLLGDHVMREANHFLRLLRDFEQGFTREFH"
     gene            complement(2406..3290)
                     /locus_tag="VF531_13460"
     CDS             complement(2406..3290)
                     /locus_tag="VF531_13460"
                     /inference="COORDINATES: protein motif:HMM:NF022604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX7715015.1"
                     /translation="MGETPNPKVFRPEVLRALLDEMQFWNPILEEHSCLIRSSLDWTE
                     DQAFDEAQQYSAFYRKLVNGIRHTTPNANPQFNTVLFRRSNNLAGGLREFLFELETGI
                     RDCRIKATLPVEAIEHLRMELDYFHAKLNGSGGVSLLTRQNMEFLVSGSLRPRMLLEK
                     LDSPENTVIIRGELLFWLKISTGHTAFLADRFRPREQLQFAQEVILFEESFDRLYRKI
                     HQNPDQTVSEADTRVAWELNQNWMNYLSELNRLLNSCQIPGRQTNYWPALGMHIYREQ
                     SYFQSVLQILIPGIQLTE"
     gene            complement(3628..4185)
                     /locus_tag="VF531_13465"
     CDS             complement(3628..4185)
                     /locus_tag="VF531_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7715016.1"
                     /translation="MPHKAVFLDRDGVLNDNRRPVNRPEDLILFPWAAAAVANLNSAG
                     FFVFIVTNQGGIELGYITPAQLEAIHQRLLALLREKNAVIDRIAYCPHFHKKCRCRKP
                     HPGMLLELSAAHHVQLTDSWMIGDREEDIGAGNSAGCRTIKLGEPVPHATYTCGNLAQ
                     AVEYILDRQQGSRPLGRAAIGNRIL"
     gene            complement(4282..4935)
                     /locus_tag="VF531_13470"
     CDS             complement(4282..4935)
                     /locus_tag="VF531_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7715017.1"
                     /translation="MPKVICVYSSSSCKIDPVYFEAAADLGREIALRGDVLLYGGGVN
                     GLMGAVAQSVHQYHGRVIGIIPEALNLDGVVYSHCDELVVTRDLRERKAVMDARSDAF
                     IALPGGFGTLEETLEIITLKQLRYHNKPIVILNCNGFFDKLLEQFDQIIAERFAKKES
                     SLLYFITASVPKALEYIDTYEPPVISAKWLTDIDPGNAAASPEKSGRNNQKEPESRK"
BASE COUNT         1397 a         1146 c         1304 g         1422 t
ORIGIN      
        1 ggcgcatgat atcacgggct aaaatatagg agacccgact ggtgcagccg cccaattcgt
       61 cgatgtcgcg attgaaatga tcgggatggt tatgagaggc gaatacatac aagtcagtgt
      121 cttgaatcag tttacgcacg atattacggc ggacttcatc caggaattgg gcggcggggt
      181 aatcgaatag gagcgcctcc tgtcccatcc tgacgatgaa ggaattatga aagatgtaag
      241 taatttcaat ccgcatggaa ataacctcct ggtttagctt tataactgat tataccatga
      301 gtttctcgcg gaaatttcct ttgaatttga atgattcggg atggatatgg aaaggaaaat
      361 atttaatcgt gaaagaggat tttggtatat tttggttaat tatttattga gcaatttttt
      421 tgaaaaatat aataaggggg taatgatttt gcgaaagata tttttgctat tattatcttt
      481 actctttaca gtattcgttt tatccggatg tagcaacctt caggaacccg ccaagccgga
      541 ggacaacctg ttgattggtc gggttacact tcaagcccaa aattttgaaa acttcggtaa
      601 tatatcgatt aatggttcga ttagtaagaa tattcaggtt acactgatta atacagaaac
      661 gggtaaaaga ttcaacgtta caacggcaga tgaaggggtt ttctcaaaaa ggaattgtga
      721 accgggtcag taccggattg aaaagttttt atatcaagct aacgcttctg attcacagtt
      781 tcggtataca agcaatattt actgtaatgt acagtccgaa cagattatca caatctccac
      841 gggtgtagta atcgatttgg ggtccttagt ttggtattgc gatggcgctt tgaaccgaag
      901 ctggtttcaa ccggaaggat ctccggaggc tttgaaagat tattttaccc aattgtttcc
      961 cgagagtctc tggctggagc gggaatgggt aatggctcaa ttaacaggga aataacggtt
     1021 cgaatcggtt tgagaaaacc ccttctctta agaggggttt ttttattttc ctaccacctt
     1081 tcttccctga gtcacgtaca atataggggc atctgtcctg agactttggg aggaggtttt
     1141 tggtatgact tcggatcagg aattcatcgt tcagtccctg gagttgaatc tattcttcat
     1201 gcgaatcgcc aaggagcatt cattctttat agaagtggcg attccttcga aaaatttcga
     1261 cctcattgcg caagcggatt cctttaagaa tcgatttgcc ggtttattga cggagaccac
     1321 ctgtttggcc gatggttatt taaccccgga gtttttaaat tcgggtgaaa tggtgaccga
     1381 cttaactctg gaggcggaac ggggcagcga attttactcc ggatacactt tgaataccga
     1441 aattaccttg gcggaacgaa ccttaagtgg gggattaagc tcatgcgagg ctaccacggt
     1501 gcaattggtg ggttgtttga atgcgcaggc catgacagcc acccgggagt tgattgattt
     1561 taaagcccgt ctattgagtg atgtcttggc ttgtcaagtc tttaccttta attatccgtt
     1621 gcttttggat catattttgc gggaagccag gttttactta tgcatgctag agaggatcca
     1681 atgccgtcaa gaacggaatc tggtggctga agcggtcgaa caggagatct tctggaaccg
     1741 gatcatggcc gagcatgcca agtttatccg gggattggtt gatcctaccg aagaacaact
     1801 ttttacgact gcggataact ttgggaaaca gtttgatggt ttaaccctcc aagctcaaac
     1861 actgcttact caaactgccg gtttgcccaa tgtgaccgag gagagccggc gggctaccgt
     1921 agcgatccgg gattttaaga ggcagggaac cgagggcttg attacctgcc gcattcggtc
     1981 cattgcccat ccgttgctgg gggatcatgt catgcgggaa gccaatcatt tcttgagatt
     2041 gcttcgggat ttcgaacagg gtttcacccg ggagttccat tgagagtcga ccgggaacag
     2101 gaaatcggtg agttgctgag attaacaagt acccgtaact tagtcgtcac gggtacatca
     2161 ttttattcac aaaggatttg gttgttaatt gtcaaatccg ccgatcaaca gaatgaggat
     2221 taaaaagatg ataaacaccc agctgccacc gaaaccagta cccacacttc ccatagtgga
     2281 gcctccttta agtattgtaa tgttctagta taaactatgg cagaactggg gagggatgaa
     2341 caggtcagtg gtattttcta ttgttataat ggcagaattt agtaaacggg atttttagac
     2401 cggttttatt cggtcaactg aattccgggg atcaaaattt gtaacaccga ttggaagtaa
     2461 ctttgttccc tgtagatatg catacccaga gccggccaat aattggtctg gcgtcccgga
     2521 atctggcagg aattcaataa ccggttgagt tccgaaagat aattcatcca attttgattg
     2581 agttcccaag ccacacgggt atcggcttcg ctgaccgttt ggtccggatt ttggtggatt
     2641 ttccggtata aacgatcaaa ggattcctca aaaagaatga cctcctgtgc aaattgcagt
     2701 tgttccctgg gacggaatct atctgcaaga aaggctgtat gcccggtgga gatctttaac
     2761 cagaacaata attccccccg gattatgact gtattttcag gggaatccag tttttccaaa
     2821 agcattcggg gacgcagcga tccggaaacc aggaattcca tattctgtct cgtgagcagt
     2881 gaaactcccc cggatccatt cagtttggca tggaaataat ccaattccat cctaagatgc
     2941 tctattgctt caaccggcaa agtcgcctta atacgacaat cgcgaatccc ggtctccagt
     3001 tcgaataaaa attcccttaa tcccccggcg agattattgg aacgccggaa caacaccgtg
     3061 ttaaactgag ggttggcatt tggggtggta tggcggatgc cgttaaccaa tttacggtaa
     3121 aacgcgctat attgttgggc ttcatcaaag gcctggtctt cggtccaatc taagctactc
     3181 cgtatcaaac aagaatgttc ttctaagatc gggttccaga actgcatctc atcgaggagc
     3241 gctcgtaaca cctcgggtct gaatacctta gggttcggag tttcacccat ggcgattgcc
     3301 cccttgataa ttattggcca cgccgttaaa ccggcatttg tccagtaatg attatgttgg
     3361 gggggattaa atcataacta ggcaataggg aacaggcaac aggcaacagg caacaggcaa
     3421 caggcattgc gtctagcgag acgcgatgca actcaccccg cggcttctat tagctttcag
     3481 tataacccta cgcccctctc tttctgaaaa gagaggggtc ggggggtgag ttgccaatag
     3541 gcaataggta acagggaaca ggcaataagc aacagggaaa agggtgaaac cgaacatgac
     3601 ccaagggaga ggtctgttat attatcttta taaaattctg ttgcctattg ccgcgcgccc
     3661 caaagggcgc gacccctgtt gcctatctag aatatactcc accgcctgag cgagattacc
     3721 gcaagtatag gtggcatggg gaacgggttc tcccaacttg atggtgcggc aacctgccga
     3781 gttgccggcc ccaatatctt cctcccggtc cccaatcatc caggaatctg tcaattgaac
     3841 gtgatgggcg gcgctaagtt cgagtaacat gccgggatgg ggcttgcggc aacggcattt
     3901 tttgtgaaaa tggggacagt acgcgattct gtcgatgacc gcgtttttct cccggagtag
     3961 ggcaagtagc cgttggtgga tggcttcaag ctgagcgggg gttatataac ccaactcgat
     4021 gccaccctgg ttggtgacga taaagacgaa aaaaccggcc gaattcaagt tggcaactgc
     4081 cgcagctgcc caaggaaaaa ggatcaaatc ctccgggcga ttgacaggtc gccggttatc
     4141 attcaaaaca ccgtcccggt caagaaagac tgctttgtga ggcatttttc cgcctccttt
     4201 catacttctc ttcatccatc gatggagagg atctccaggt tgggcctttc taattccggg
     4261 tcattcgcga tttgttgatc cttattttcg ggattcgggt tctttttgat tgttccgccc
     4321 ggacttctcc ggggaagccg cggcgtttcc cggatcaatg tcggtcaacc atttggcgct
     4381 gataaccggc ggctcatagg tgtcgatata ctccaaggct ttggggacgc tggcggtaat
     4441 gaaatacaac aaggaagatt cttttttggc gaatcgctcc gcgattatct gatcgaactg
     4501 ctccaatagt ttatcgaaga atccattgca attaagaatg acaatgggtt tattatgata
     4561 ccgaagctgt ttgagagtga tgatctcaag cgtctcctcc aaagtcccaa aacctcccgg
     4621 tagggcaatg aatgcatccg agcgagcgtc catcaccgct ttacgttctc taagatcgcg
     4681 ggtgaccacc agttcatcgc aatggctata aactactcca tcgaggttta gcgcctcggg
     4741 aataatcccg ataacccggc catgatattg atgaacactt tgagctacgg ctcccatcag
     4801 gccgttgaca ccgccgccat aaagcagcac atccccgcga agtgcaatct cccttcccag
     4861 atcggctgct gcctcaaaat aaaccggatc gatcttgcag ctggatgagc tgtatacgca
     4921 gatcacttta ggcatggctg aatcctttcg gataacatga ttaaagtaaa tcatatcgga
     4981 tttgaggcca taagtaaagc gggactccgt atgcctcaaa attagggata catttgatta
     5041 cggcggtgcg tttaggggat aattttaaaa gatacgaaag gataacgtat tttaggatag
     5101 aatattatgc aaccgatagg taaaaaaagg aggaaattca agatgaatcc cacggatact
     5161 aaacacctcc cccgtagaga ggagattcct gttgcctttc aatggaatct ggcggagatc
     5221 tatccctctc tagatgcttg ggaagcggat ttcaagcgtt tgaaaggaa
//
LOCUS       DASVMB010000480         6202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_720677,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000480 DASVMB010000000
VERSION     DASVMB010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720677"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..808)
                     /locus_tag="VF531_13475"
     CDS             complement(302..808)
                     /locus_tag="VF531_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715018.1"
                     /translation="MPSDMKNLQPPWIQSLLRELAAIDFLADDQELLCSTVCESTISD
                     AEYLDIPDLEDEDDFAVELGKLINQYWHGTKRQLCSDALLLENTFYKIQSGRRQPGRD
                     TIICLALTLHLTPEETQHLLGYLGYGLSSNIKRDYIILRCLELKKKPLEVNLVLETSG
                     LRLLGMEN"
     gene            complement(927..1340)
                     /locus_tag="VF531_13480"
     CDS             complement(927..1340)
                     /locus_tag="VF531_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX7715019.1"
                     /translation="MTHEQLDISRYRLGCAKEDLDACRITLEHNKLKNSINRAYYSIF
                     HAIRAVNALEGFDSKRHSGVIAYFNQHFVKTGIFEIGCSKIIENAFRIRSNADYEDFY
                     IVSTDEAKEQLQNAEVFVNAVETYLKEVWEQMKNE"
     gene            complement(1321..1689)
                     /locus_tag="VF531_13485"
     CDS             complement(1321..1689)
                     /locus_tag="VF531_13485"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7715020.1"
                     /translation="MTGLTAKSKDELDLILNEFVDKVTPLFGDKLKKVVLFGSYARGD
                     YDGESDIDVMLMLNEDEIVLKKYSDQITDIVVELDLKYNVVLATILQSEKKFGKYQHA
                     MPFYSSVASEGVVLYDSRAT"
     gene            complement(1920..2774)
                     /locus_tag="VF531_13490"
     CDS             complement(1920..2774)
                     /locus_tag="VF531_13490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7715021.1"
                     /translation="MNKRIDREVPETPQDPQTIKQQQYANAGKFNARIQLHAECATNK
                     YPWPLWIFDQLEKRPNQKILELGCGTGLLWQVNGTRIPWDWEITVSDFSGGMLAQTRQ
                     NLQSLKTQFQYELVDAENIPFPENHFDIVIANHMLYHVPDLPKALSEISRVLKSDGTL
                     YASTIGANNMRELKQLLYGFLQNHNYEAAVGSVEKNFSLDNGKDHLHQYFQDIRIVRY
                     ENALNITNPDLLVNYVTSCNGLKPGVEVLAENSVEKFRGYVENLINVNGRILVATESG
                     MFICKQRG"
     gene            complement(2876..3739)
                     /locus_tag="VF531_13495"
     CDS             complement(2876..3739)
                     /locus_tag="VF531_13495"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7715022.1"
                     /translation="MNYFQMIGDTIAYIETHLDQNMTVDELASRHHISKFYFTRIFKA
                     VTRKNVKEYIDGRRMAEAAQKLRKNSARIIDVALEYGFESHEAFTRKFKNLYGITPGE
                     YKKSPRELPTCERIEVVERQFKNLHRDLIVDFQIESPGTIQLTGKHIRFNPDNPAELP
                     KVKAFFNQFIARYIAAKGIDRIYNVTGGEAAPGETIDYFAGYQPQDQETHQSYRIGLT
                     PLTLEASDYAVFRYKNTMEGIHRLVASDVWKAILLSDLTLNQIGIHFFELYERGYDQT
                     NEFQLYVPIKS"
     gene            3861..4967
                     /locus_tag="VF531_13500"
     CDS             3861..4967
                     /locus_tag="VF531_13500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7715023.1"
                     /translation="MIKFNLKLKYLLPLLVIFFMPSLYATSEKTTAPVTKNVIIPILC
                     YHRVIPNANNIYDITPETLEKQLRFMQDHGYHPITALQFISLQKSPQLLPDKPVVITF
                     DDGCKSHYQNVFPILKKLGLKATFFVYPSAVLNKESKKYITWPELQEMVQAGMDIECH
                     TLYHPFLVRAHTNITDPKYLQWLNHQLKDSKTILENHLNIKVKLLAYPFGRFNRLIES
                     KSIEAGYEGIFTINGGTNFLHENPMRLKRLIVNRQISTAGLESFFHTKPLPLEITSPI
                     DTSIVAECPVIEFRLAGLKLPKVHIQVGNDNADLYPDANGNFTYQIKKPEIGFNLITI
                     SGYDEASTHGKDFYLNSWSFDYLIQDKTNDMTKY"
BASE COUNT         1656 a         1435 c         1359 g         1752 t
ORIGIN      
        1 agaaaccaag ccgaacccgt cgttataaac caccaggtat agatcttgat tatccttcat
       61 tgaggatgtt ttcatgacat tcagctccct ccgcgtttgc tatgtccatc ataacgccgg
      121 ggaaggggtg aatgtatgga caggaagtcc agaccccacc tctttggcgc gtacaaagta
      181 cgcgactcct ccccgttcgg agaggagtca cgtccgttgg acgtgcaccc atatgctttg
      241 gagggattaa ggcagcaggg gagaacgttt agatgtattg gggtacagat taacaaaaag
      301 ttcagttttc cattcccagg aggcggaggc cggaggtttc gagtaccagg ttgacttcca
      361 aaggtttttt cttcagttcg aggcagcgaa ggatgatgta atcccgtttg atgttggagc
      421 ttaggccgta acccaaataa cccagtaagt gttgggtctc ttcgggggtc aaatgcaggg
      481 taagggcgag gcagatgatg gtgtcgcggc ccggctggcg ccggccggat tggatcttgt
      541 agaaggtgtt ctcgaggagt agcgcgtccg agcagagttg gcgtttggtt ccgtgccaat
      601 attggttgat gagtttgccg agctcaactg caaaatcgtc ctcgtcctcc aggtcgggta
      661 tatccaaata ctccgcatcg gaaatagttg actcgcaaac agtagagcat agcagttcct
      721 gatcgtcggc aaggaagtcg atggcggcga gttcacgaag gagagattga atccaggggg
      781 gttggagatt tttcatatct gatggcaagg cggttcatcc tcgttcatcc gtttgcaatt
      841 actgtataaa aacatttctc aagcggtccc tcatatgctg tagttatcat ggcatcgagc
      901 agttcgttac ttccaacaaa cgaccactat tcattcttca tttgctccca aacttctttt
      961 aaataagttt ctacagcatt gacaaaaact tcggcatttt gaagttgttc cttggcctca
     1021 tcagtgctaa ctatgtaaaa atcctcataa tcggcgttac ttcttattcg aaacgcattt
     1081 tcgattattt tagaacagcc tatctcaaat atacctgtct taacgaaatg ctgattaaaa
     1141 taagcaatta ctccggaatg tcttttggaa tcaaagccct ccaaagcatt gacagctctg
     1201 attgcatgaa aaatggagta atatgcccga tttattgaat ttttcaactt attatgttct
     1261 aatgtaatac gacatgcatc cagatcctct ttcgcgcacc caagcctata ccgagagatg
     1321 tcaagttgct cgtgagtcat ataaaaccac cccttcactg gcaacacttg agtaaaacgg
     1381 catggcgtgc tggtatttgc cgaatttctt ttcactctga agtatcgttg ccaaaaccac
     1441 attatatttt aggtcaagct caacgacaat gtcggtaatt tgatcggaat atttttttaa
     1501 aactatttca tcttcattta acatcaacat cacatcgata tccgattccc cgtcataatc
     1561 gcctctagca taggaaccga aaagaactac ttttttcagc ttatccccaa ataacggagt
     1621 tactttatcc acgaattcat ttaagatcag atcaagctca tcttttgact tagctgtcaa
     1681 gccggtcatt ttgatcacct caatctccgc tccgtacttt cagatctata ttattattat
     1741 ttatatttta cattgtaaat catagattgt acagacgact acagcacgtg cccacccgca
     1801 cgcgtccgtc ttcttggaaa atcatgtatg ccgacggcgc agtttgtgga aagtcgaatt
     1861 tcatccggaa tcgaaaccgc ttttctcatc tcaccggtat tatataccag actctcccat
     1921 taccctctct gtttacaaat aaacatcccc gactccgtcg caacaagaat ccgtccattg
     1981 acgttgatca ggttctccac atatccccgg aacttttcaa cactattttc cgcgagcact
     2041 tcgactccag gtttcaatcc gttacacgaa gtaacataat tcaccagcaa atccgggttg
     2101 gtaatattca aggcattctc atacctgact atccgaatat cctgaaaata ctgatgtaaa
     2161 tgatctttgc cgttatccag ggaaaaattc ttttcaaccg atccgacggc tgcttcataa
     2221 ttgtgatttt gtagaaaccc gtaaagcaat tgtttcagct cgcgcatgtt attggcgcca
     2281 atggtactgg cgtaaagggt gccatcgctc ttcaggactc gggagatttc ggacaatgcc
     2341 ttaggtaaat cgggaacatg gtataacata tgattcgcga tgacgatgtc aaaatgattc
     2401 tccggaaaag gaatgttctc ggcatcgacc agttcatatt gaaattgagt ttttaaagat
     2461 tggagatttt gccgggtttg ggctaacatc ccccccgaaa agtcggagac ggtgatctcc
     2521 caatcccaag gaatccgtgt gccgttgacc tgccaaagca gtccggttcc gcaacccagt
     2581 tccaggattt tttgatttgg cctcttctcc aactgatcaa agatccacag cggccaagga
     2641 tacttgttgg tggcgcactc tgcgtgaagt tggatgcggg cgttaaattt accggcgttg
     2701 gcatactgct gttgtttgat ggtttgaggg tcttgcgggg tttctggtac ttcacggtca
     2761 attcgtttat tcataatcat cctctttcct tgcggtagct tgccgcgcac gcgcggattt
     2821 cttcaccaag cataccatct acgggaaaag cggacttgac catttttgca gtattttatg
     2881 atttaatggg aacatacagc tgaaactcgt tggtttgatc gtacccccgc tcgtaaagtt
     2941 cgaaaaaatg aatcccgatc tgattcaacg taaggtccga cagtagaatc gccttccaaa
     3001 catcggaagc caccaggcga tggatgcctt ccatggtatt tttatagcga aaaaccgcat
     3061 aatcggaggc ttccaaggtc agcggggtaa gcccaatacg atagctttgg tgagtctctt
     3121 gatcttgcgg ttgatacccg gcaaaataat cgatggtttc accaggagcg gcttcacctc
     3181 cggtcacatt atagattctg tcaattccct tggctgcgat gtaacgggcg atgaattggt
     3241 tgaaaaaggc ctttactttc ggcagttcag cggggttatc cggattgaac cggatatgtt
     3301 tgccagtcaa ttggatggtc cccggactct caatctgaaa gtcgacaatc aggtcccggt
     3361 ggaggttttt aaactgccgc tccaccactt caatccgctc acaggttggt agctctcgcg
     3421 gggatttttt atactcgccg ggggtgatgc catatagatt tttaaatttc ctggtgaagg
     3481 cttcatgaga ttcaaaacca tactcgagcg ccacatcgat aatcctagcg gaattctttc
     3541 tcagtttttg agcggcctca gccattctgc ggccatcgat atattctttg acgtttttcc
     3601 tggtgacggc cttgaaaatg cgggtgaagt aaaacttgga gatgtggtgg cgggaggcta
     3661 attcatcaac ggtcatattt tggtcgagat gagtttcgat gtatgcgatg gtatcgccaa
     3721 tcatttgaaa gtagttcatc tattttatga ccccacctgg cattaaattt attaaaactg
     3781 gtgaatcccg gttgcaaatt tataaaatat gaactatcct ttattagcat aaactattct
     3841 atcaaacacg aggcacagct atgataaaat ttaatttaaa gctaaaatat ttattacccc
     3901 tgctggtaat tttttttatg cccagtttat atgctacgtc agaaaaaact accgccccgg
     3961 taactaaaaa tgtcatcatt ccaattctat gctaccaccg tgtaattcca aatgctaata
     4021 atatctatga tattaccccg gaaactctcg aaaaacagtt acgatttatg caggaccatg
     4081 gctatcaccc aataacagcc ctacaattta taagtttgca aaaatccccc caacttcttc
     4141 ctgacaaacc agtggttatt acttttgatg atggatgtaa aagtcattat caaaatgttt
     4201 ttccaatcct gaaaaaactg ggcttaaaag cgaccttttt tgtatacccc agtgcggttt
     4261 tgaataaaga aagtaaaaaa tatatcactt ggccggagct tcaggaaatg gtgcaagcgg
     4321 gtatggatat tgaatgtcat actttatatc atccgttttt agttagagct cataccaata
     4381 tcacagatcc gaaatatctg caatggctta accatcaatt aaaagattcc aaaacaatat
     4441 tagagaatca tttgaatatc aaagtaaaat tactggctta tccctttgga cgatttaacc
     4501 ggcttattga atcaaaatcg attgaggcag gatacgaggg aatatttacg attaatggcg
     4561 gtaccaactt tcttcatgaa aacccgatgc gcttaaaacg gttgattgtg aatcgtcaaa
     4621 tttcaacagc cggtttggag agtttctttc atacaaaacc tttacccctt gagatcactt
     4681 cgccgattga cacctcgatt gttgcggaat gtccggttat tgaatttcgt ttagccggcc
     4741 tcaagctacc caaggtccat atccaagtcg ggaatgataa cgcggactta tatccggatg
     4801 ccaatggtaa tttcacttac caaatcaaaa agccagagat tggtttcaat ctgattacga
     4861 tttccggtta tgatgaagca agcacccacg gcaaagattt ttatttaaat tcctggagtt
     4921 ttgactatct cattcaagat aaaaccaatg atatgacgaa atattaaaat acagtgaaga
     4981 tggaagccta tgaattaatc cgagcaattt tctgcaatgg tgatggcaat atagaagtcg
     5041 actccgatct cgacggggga cggcgcgaac aatgttcgcg agccaactcc cccgcacctc
     5101 ctagggccaa agggtgttac ggaacacctg cgcgatgcat aacatcacaa ggatgttatg
     5161 catcgcgagg aatcccgccg tccggggacc tggcccccgg atgccccctg atatcggcat
     5221 tcctccttga acgccgtcgg agtgctgcgc gattatacag acgcctacct ccatgtagcc
     5281 gtcttcttgg gtaattctga cggccgacgg ttgtgatata tcctaaatcg aaaccgcgtg
     5341 gtcaacctcc ctgttgacca tttcggggct atatcatata gacaatctct aagtttcaaa
     5401 gatttaatat catacccttt caaaaaactc ctttgaaagc caaaaaccac caacacggat
     5461 gttggtggcg cggcttcgat tcttggatga atcactcggc ttgtttacaa gccgcgccgt
     5521 cgaggagaga ggactaccat agaggccgga gacacgaggt cgcaggccaa taggcgtaat
     5581 aatttaacat tctagggttc acctccccgg ctatggatgg gcggggataa gggggggtac
     5641 cgggggcctc acccaaaata ggcgtcgtga catagcaatt ctcccctctc ccagtgtttt
     5701 cgtgggagag gggaataaag gggtgaggtt ggcaacttcg acagaacatg tttcccctct
     5761 cctgcttgct ttttagtagg agaggggaga aaaggggtga ggtggccggt gggttcctcg
     5821 cgatgcaagc atacgtggga ttgccggtcc aatcccttgg acctggaggt gcaggagggt
     5881 gggttcgcga acattgttcg cgccgcctcc tgtcggcagg gatgattctt aagcttttac
     5941 taaatagatc ggcgtcgcca cttggaaata acccattttc agtccttttt caagacctaa
     6001 ttttttattg acatgtttga ttccttcgaa cccaaccggg ccgtgaacgc cccggctaca
     6061 agatggttcg tgtgcaatgc acacgcgccc ccctgggtta atttgggcaa gttatctctg
     6121 aaaaacacgt cgataaacga gcaagcccac gcatttttcc cctatctctt ttcaaacttc
     6181 acacctcacc cttcaaactt cc
//
LOCUS       DASVMB010000481         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_57779,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000481 DASVMB010000000
VERSION     DASVMB010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57779"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VF531_13505"
     CDS             complement(<1..768)
                     /locus_tag="VF531_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715024.1"
                     /translation="MSVMKSQASGGTIASKNAGHSWNQLLRSYGLLGVFALMFMILFI
                     FSPNFRNPQNMLNLLQQNAVNGIIACGMTLMIITGGFDLSVGATAALSGVVAASVFLQ
                     VSIPAGICAALLASLLVGLCNGLLIAKVRISAFVATLGTQIITRGILYITTNATPIYG
                     LPSSYMRVGLGSIGPFPIATSIFFLVVVATHILLRYMPFGQHLLATGGNEEASRLSGI
                     DVDRVKIIVYSLGGLLAGLGGLILLGQTNTGQPAAANG"
     gene            complement(816..2030)
                     /locus_tag="VF531_13510"
     CDS             complement(816..2030)
                     /locus_tag="VF531_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7715025.1"
                     /translation="MYPKIGTPQFMKSMNRATIIDTLVKKSPISQTQICDITGLSRAT
                     VSTIINELKQEQLVIEVSKELSKGGRRQVLLKLDENAGYVVGIDLGGTKMVGAVTDLN
                     GKFIVKMRWPTQANEGPEAILETLIRFIKALISKAGVDFEKVRGVGVGVPGVVRPNGI
                     VEWAPNLKWRNLDLTSKLSEAIQRPVFIENDVNLLALGEYWYGAAQGAQCLACLAVGT
                     GIGAGIVINGNLFTGTHQAAGEICNLLVDRSYLAKDFSQYGCLETLASGPAIASQFAS
                     AMKNTGNKDNETTDVLERESAESSLTGAELVFKEAGQNNPIAQEIVEEFSQNIAMAII
                     ALTTVLDPDLIILGGGVSQNAGLFRDRILELCSPVVQVMPRIEVSQLGVDAGVMGAVA
                     LTLHNTSEKPWE"
     gene            2438..3280
                     /locus_tag="VF531_13515"
     CDS             2438..3280
                     /locus_tag="VF531_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7715026.1"
                     /translation="MEAWHQKNLTDETSPFQLIITDIPEFPPHWHEELEIIYVLDEDL
                     VIGLNHEVYTLKPRDIFLIGPGEVHFFINPPKKSKRVIIQFELAIFESFAARMREQKF
                     SNPLIRSIDERSHPHRGLERQILRIKEEYIRHREGHELAIKARLYDLVVILLRQVPMV
                     PYSSVAQNKHLKRLERLEQVFRYVEEHYTQRISLTEVAAAANFSIYHFTRFFKEATGM
                     TFIMYLNHYRIAKAVRYLTATTDPITEIAFKSGFESIQSFNRVFRQLKGCSPTSYIKG
                     LLAK"
     gene            3579..>4650
                     /locus_tag="VF531_13520"
     CDS             3579..>4650
                     /locus_tag="VF531_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7715027.1"
                     /translation="MGYTNFDAAIYFTVNDVEKFAQLADPNQELAFILKHIQLGKVYL
                     ETFRSDHWIDRDKLLHVKEYFIQKNIRTSGGFTTTKAGLGEFKTFCYTNEEDCNLLRQ
                     VVEFTAAFFDEIILDDFFFTNCKCESCIRAKGERSWPQFRTALLKQVAEEIVLATAKR
                     VNPNVRVIIKYPNWYEDYQSTGYNLEDESRIFDGIYTGTETRDPDTTQQNLQRYQSYF
                     IMRYLESVKPGKNGGGWFDSFDCQYNLNSYAEQCYLTLFAKAREVTFFCAGLLFRRDR
                     IVVPIAGYVLETADSFLGELGKPEGIACYKPFDSHGGSESYIHGFFGMLGLPLEPAPH
                     YPEDRELILLTKNAGYDLHLVER"
BASE COUNT         1254 a         1153 c         1014 g         1229 t
ORIGIN      
        1 tccattagcc gcagctggtt ggccggtgtt ggtttgacca agtaaaatca agcctcctag
       61 accggccaat aacccaccca ggctataaac gataatttta acccgatcaa catcaattcc
      121 tgaaagtctt gaagcttctt cattcccccc ggtggcgagc agatgttgtc caaacggcat
      181 ataacgcaat aaaatatgtg tagcgacaac taccaggaaa aagatactgg tggcgattgg
      241 aaaggggccg atactgccca acccgaccct catgtacgaa ctcggtaggc cataaatcgg
      301 tgtcgcattg gtagtaatgt acaagattcc ccgggtaata atctgtgtgc ccaaggtggc
      361 cacaaaagcg ctaattcgga ctttcgctat caataaaccg ttgcataatc cgaccagaag
      421 tgaagcgagc agagcagcac agatacctgc aggaatgctt acttgtaaga aaacagaagc
      481 ggcaactact ccggatagtg cagcggttgc ccccacggag agatcaaatc caccggtaat
      541 gatcatcagc gtcataccgc atgctatgat gccgtttacc gcgttctgct ggaggagatt
      601 caacatattt tgcggatttc gaaagttcgg tgagaagata aaaaggatca taaacatcaa
      661 agcaaataca cccaacaaac cataggacct taacaattga ttccacgaat gcccggcgtt
      721 tttggatgca atcgtcccgc cgctcgcttg acttttcatg acactcatcg ttatccccct
      781 taatggctta ggtctagccg tcttggaaaa aatcattatt cccagggctt ttcactggta
      841 ttgtgtaatg taagggcaac cgcgcccatt accccggcgt ccaccccaag ttgagagact
      901 tcaatcctag gcattacctg gactaccggg gaacaaagct ccaatattct atcccgaaaa
      961 agcccggcgt tttgggatac tcccccgcca agtatgataa gatccgggtc caggacagta
     1021 gtcaaagcaa tgatggccat tgcgatgttc tgagaaaatt cctcgacgat ctcttgggcg
     1081 atgggattgt tttgaccggc ttctttaaag accaattcag cccctgttaa actggattcg
     1141 gcggattccc gctctaatac atccgtggtt tcattatcct tatttccagt gtttttcatc
     1201 gctgaggcga attggctggc aattgccgga ccgctggcca acgtttccag gcagccatat
     1261 tggctaaagt ccttagccag gtatgaacga tcaaccaaca ggttgcagat ttctcccgca
     1321 gcctgatggg tgccggtaaa aagatttccg ttgatgacga ttccggcacc aatacccgtg
     1381 cctacagcga ggcaggcaag gcattgagct ccttgagccg ctccatacca atactcaccc
     1441 aacgccaata gattcacatc attttcgatg aataccggcc tctggatggc ttcactgagt
     1501 ttcgaagtta aatcaagatt acgccattta agattgggcg cccattccac aataccgttg
     1561 ggccgcacaa ctccaggaac tccaactccg acgcctctta ctttttcaaa atccactcca
     1621 gctttactaa tcaacgcttt aatgaaacgg atcaaagttt ccaaaattgc ttccggtcct
     1681 tcgttggcct gggtcggcca gcgcatcttg acgatgaatt tgccgtttaa atcggtaacc
     1741 gcgccgacca ttttcgtacc gcctaagtca attcccacta cataacccgc attctcatct
     1801 agttttagca aaacctgcct gcgccctcct ttggataact ctttgcttac ttcaatcacc
     1861 agttgttctt gctttaactc attaataatt gtggatactg tcgctctgct caggccggta
     1921 atatcacaga tttgggtttg cgatatcgga ctttttttaa ctaatgtatc tattatcgtt
     1981 gcgcggttca ttgatttcat aaactgtggc gtcccaattt tcggatacat tttacccctc
     2041 caacagcaga caattttgat tacagtaaag aacaagtatg taataacgcc ggctggacag
     2101 taaccgtaaa ctgtaatttt actttattta ttctccgaac aaactaatat tccttctgga
     2161 tcatttgatt ttttttacta ttgaaattgc ctgttatatc catcaaatac ttactttgta
     2221 tcctaaacaa atataacaat gtcggatttc ggctgaaata tttacttcga ttaaagatcc
     2281 ataagagctt gaaataataa tctaaataag cgctttggga ataaaggaaa atttgttgaa
     2341 ggaaggatta ccatacccga agggtgaatt aatctcgggt tctgattttg catgattcga
     2401 tcgcgaagtc taaactttac cagtgggggc agaactcgtg gaggcttggc atcaaaagaa
     2461 tttaacggat gaaacatccc cctttcaatt gatcatcacc gatatcccgg agttccctcc
     2521 ccactggcat gaggagctgg aaatcattta cgtgcttgat gaggacctgg taattggttt
     2581 gaatcatgaa gtctatacgc taaaaccccg ggacatcttt ttgatcggcc cgggggaagt
     2641 ccatttcttc atcaatccgc cgaaaaagag caaacgggtg attatccagt tcgaattggc
     2701 gatctttgaa tccttcgccg cccggatgcg ggaacaaaag ttttccaatc cgctcattcg
     2761 gagcatagat gaaagatccc atccgcaccg gggattggaa cggcagattc ttcggataaa
     2821 ggaagagtac atccgtcatc gtgaaggtca tgaattagcc atcaaagcac ggctctatga
     2881 tctggttgtg atcctgctgc gtcaggtccc gatggttcct tattcttccg tggcccagaa
     2941 caaacatttg aaacgtttgg aacgactgga gcaggtcttc cggtatgtgg aggagcatta
     3001 tacccaaagg atctctctca cagaggtcgc ggcagccgcc aatttcagta tttaccattt
     3061 cacgcgtttt ttcaaggaag ccaccggtat gacattcatc atgtacctga accattaccg
     3121 gattgccaag gctgttaggt atctgaccgc aaccacggat cccattacgg aaattgcttt
     3181 caagtcgggt tttgagagta ttcagagttt taaccgggtg tttcgccagt taaaaggttg
     3241 ttcccctacc tcatacataa aagggctcct tgccaagtaa tacccgcccc ggtacagaac
     3301 aatcagccgc ttcactcaaa ttgcgtcccc gtccaaccat tccaatccca ttcttcctgc
     3361 aattttacgg ctgccagtcg aactccggca tattgctatt cttatcgtaa tatcaaaata
     3421 ccgatctttt tctttattaa gcctatttaa aacatgtctt ataaaatcga aaaacagcaa
     3481 aatccgagta gcctttcgca atccacggcg agatcctcca tcaatgccgg attataataa
     3541 aaaccataat cattgcggaa aaggaggacg cttggaatat gggttacacc aattttgacg
     3601 cggcaatcta tttcacagta aacgatgtgg agaagttcgc acagcttgcc gatcccaacc
     3661 aggaattggc ctttatccta aaacatatcc aactgggcaa ggtttatttg gagacattcc
     3721 gcagcgacca ttggattgac cgcgacaaat tacttcatgt caaggagtac ttcattcaaa
     3781 aaaacatccg gacctcgggt ggttttacca ccaccaaggc aggtctgggt gagtttaaga
     3841 ccttttgcta tacgaacgaa gaagattgta acctgctccg gcaggtggtg gagttcactg
     3901 cagcgttttt cgacgagatc atcctggacg atttcttctt taccaattgt aaatgcgaat
     3961 catgcatccg ggccaagggc gagcggagtt ggccccagtt tcgcacggct cttttaaaac
     4021 aggtggccga agagatcgtg ctggctacgg caaaacgggt caaccccaat gtcagggtga
     4081 tcatcaagta tcccaactgg tatgaagatt accaaagcac cggttataac ctggaggacg
     4141 aatcaaggat ttttgacggg atttataccg gcaccgaaac ccgcgaccct gatactaccc
     4201 aacaaaatct ccagcgttac cagagctatt tcatcatgcg gtacctggag agcgtcaaac
     4261 ccggcaaaaa cgggggtggt tggttcgaca gcttcgactg ccagtataat ctgaattcct
     4321 atgccgaaca gtgttatctg accctctttg ccaaagccag ggaagtcact tttttctgcg
     4381 ccggattact ctttaggcgc gatcggatcg tcgttcccat tgccgggtat gtcctggaaa
     4441 ctgccgatag ctttctgggg gaactcggta aaccggaagg aatcgcctgt tacaaaccgt
     4501 ttgattctca cgggggttct gaaagttaca tccacgggtt ctttggaatg ctgggtctgc
     4561 cacttgagcc ggcgcctcac tatccggagg atcgtgaact catccttttg acgaagaacg
     4621 ccggatatga cctccatctg gtagaacgaa
//
LOCUS       DASVMB010000482         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_499627,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000482 DASVMB010000000
VERSION     DASVMB010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499627"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF531_13525"
     CDS             <1..199
                     /locus_tag="VF531_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7715028.1"
                     /translation="CQRLWLITTNDNLAAIRFYQLRGFVLVALHRDAIAQSRKLKPEI
                     PLRGIDGIPIRDEIELEVVLK"
     gene            481..1857
                     /gene="argH"
                     /locus_tag="VF531_13530"
     CDS             481..1857
                     /gene="argH"
                     /locus_tag="VF531_13530"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7715029.1"
                     /translation="MKAWGGRFQKETAALMDDFHSSIHFDYRMAEEDIGGSIAHARML
                     GATGIITPAEAETIILGLHKILERVLAGEIVWDPKAEDIHMNVEKLLIAEIGDLGKKL
                     HTARSRNDQVALDARLYQLKEIRSIRSLLIELARTLVQLAERERETILPGYTHLQRAQ
                     PVLLAHHLLAYFEMFRRDLERLGDCSGRTAVSPLGAGALAGTGFPINREQVATELGLA
                     GVVLNSLDAVSDRDFVAEFIFDVSLVMMHLSRFCEELVLWSTMEFGFVDMDDSYSTGS
                     SIMPQKKNPDVAELVRGKTGRVYGDLITLLTVMKGLPLAYNKDMQEDKEALFDAADTV
                     KGALSIFTPMLATLVFKRERMLAATRDGFLNATDLADYLAKKGMPFRAAHEVVGKLVK
                     HCINTGKRLEELTLPELQSASGLISGDVYQVLEMKRVVEARSSYGGTSFAQVTAQLAR
                     AKEWLESL"
     gene            1887..2408
                     /locus_tag="VF531_13535"
     CDS             1887..2408
                     /locus_tag="VF531_13535"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7715030.1"
                     /translation="MDLKPLIREVPDFPRPGISFKDITTLLKDGAALRQVVDAFAEHF
                     GPTKPDLIVGAESRGFLLGAPLAYKLGCGFVLVRKPGKLPAAVAKVSYDLEYGQDSLE
                     IHLDAIKPGQRVVIVDDLLATGGTISATAELVKKLKGEIIGFAFIIELDPLKGREKLK
                     GYDVLSLVHYDSD"
     gene            2603..>3452
                     /locus_tag="VF531_13540"
     CDS             2603..>3452
                     /locus_tag="VF531_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715031.1"
                     /translation="MKSKFLINLGFICLLVLASGIITPVQTEVIQAGAGAVIISPMPD
                     RYFKTGTNLYYGISAAHGFMVSTDGRGWVERNQGLPQKNVYPFKEPRPRPLTALGVDP
                     LDPLKVAVTTSTRLYFSENGGESWREIPLHAPISGGTYLTSVALTTVATKVHMLIGTA
                     FAGIYESINLGQSWSSITPRLRFLYQGAGFWEEIAGIVYSPEEPGVIYFAAGFGHGLY
                     RWDRTAQKVTRLDSATPVTINTAVEEALNPGVATNPEGFQPLVLHWIPDPEPVAPDLK
                     PNWILEE"
BASE COUNT          785 a          832 c          975 g          860 t
ORIGIN      
        1 gtgccaacgg ctctggctga tcaccaccaa tgacaatctg gcggcgatcc gtttctacca
       61 gttgcggggt ttcgttttgg ttgccctaca ccgggatgcc attgcccaat caagaaagct
      121 gaagccggag attccattga ggggaatcga cggcataccg attcgggatg aaattgaatt
      181 ggaggttgta ttgaaatagg tgaaaatgcg actcaatcgg ttcagaatgt gagtataata
      241 cttatggacc cgatcaaccc ccttccctgt cccggatgca taccaacaga atgagggaag
      301 ggaatggggg ttaggtaaaa aagaagggtt ttccctattt ttgtggaata atctgtcgtc
      361 aatcaatatg atgcttcctg ctcgcgttcc tttaatggac tgcgagaggg tggctttatt
      421 cgtttttttt ggaataatga atagatgccg gttccaatct aacttatcag gaggcgcttt
      481 atgaaagcct ggggcggacg ttttcaaaaa gagaccgctg ccttgatgga cgattttcat
      541 tcctcgatcc atttcgacta taggatggcc gaagaggata ttgggggaag catcgctcat
      601 gcccggatgc tcggggctac cggaattatc acccccgccg aagctgaaac catcatcctt
      661 gggttgcata aaatcttgga gagagttctt gccggagaga ttgtgtggga tcccaaggcc
      721 gaagatattc atatgaatgt cgagaagtta ctcatcgcgg agatcggtga cctcggcaag
      781 aaacttcata ccgcccggag tcgcaacgat caggtggcgc tggatgcccg gttgtatcag
      841 ttaaaggaga tccggtccat ccggagcctt ttgattgaac tcgcccgaac cctggtgcaa
      901 ctcgcggaga gagaacggga gactatcctg cccggttata cccatttgca aagggcgcaa
      961 ccggtgcttc tggcccacca tctcctggcc tatttcgaga tgttccggcg cgatctggag
     1021 cggttgggtg attgttccgg aagaaccgcg gtctcgccgc taggagccgg ggctttggct
     1081 ggaaccggtt ttcccattaa tcgggagcag gtcgcaacgg aattggggct cgccggcgtg
     1141 gttctcaata gcctggatgc cgtcagcgac cgggatttcg tggccgaatt catatttgac
     1201 gtttcgttgg tcatgatgca cctatcacgt ttttgtgagg agttggtcct ctggtccact
     1261 atggaatttg gtttcgtgga tatggacgac tcctacagca ccgggtcgag catcatgccg
     1321 cagaaaaaga atcccgatgt ggccgaattg gtccggggca agacgggacg tgtttatggg
     1381 gatctgatca cgttgctcac ggtgatgaaa gggctgccgc ttgcatacaa caaagatatg
     1441 caggaagata aagaagcgtt gtttgatgcg gccgatacgg tcaaaggggc cctttccatc
     1501 tttacaccga tgctggcgac gctcgttttc aagcgggaga ggatgcttgc cgcaacccgg
     1561 gatggttttt taaacgccac cgatttagcc gattaccttg ccaaaaaggg aatgccattt
     1621 cgcgctgccc acgaggtcgt tgggaaactg gtaaaacatt gtatcaatac gggtaagcgc
     1681 ctggaggaac tgacgctccc ggaattacag tccgcatccg gtttaatttc cggagatgtt
     1741 taccaggtcc ttgagatgaa acgggtggtt gaggcgcgtt cctcttacgg agggacctcc
     1801 tttgcccaag taacagcgca gttggctcgt gccaaggaat ggcttgagag tctttaatat
     1861 ctatgataaa gtgaggcgct atcaccatgg atttaaaacc tttgattcgg gaggtccctg
     1921 attttccgag accgggcatt agctttaaag atatcaccac tttgttgaag gacggtgcgg
     1981 ctcttcgcca agtggtcgat gcgtttgccg aacattttgg gccaacgaag ccggatctga
     2041 tcgtaggcgc cgaatcgcgg ggcttccttc ttggtgcgcc cctggcctac aaattgggat
     2101 gcggtttcgt tttggtccgt aagcccggca aactgccggc cgcagttgcg aaagtatctt
     2161 acgatctgga gtacggtcag gactccctgg agattcacct ggacgccatt aagcccgggc
     2221 aacgggtagt tatcgtagac gatcttctgg caaccggcgg gacgatctcc gctactgcgg
     2281 aactggtaaa gaaactcaaa ggcgagatca tcggatttgc cttcattatt gagttggatc
     2341 cgctgaaagg gcgggagaag ctcaaggggt acgatgtgct gagcctagtt cattacgatt
     2401 ccgactgagt gtccggattt tacactttat atggtaaatt aagcggcttt tttggaagcc
     2461 gctttttggt atagtcctca tccaaggcct ctcccggtaa cgtgctgacc atgatcatac
     2521 ccgggaaaat tgttttaata aacggttttc gttagccggg gccaccggtt gcaagttgat
     2581 tcccagtgga aggaagatcc ctatgaaatc aaaattcttg attaatctcg gctttatttg
     2641 tctgttagtc ctggcaagcg gcattataac cccggtccaa accgaagtta ttcaagccgg
     2701 agccggggct gtaatcatca gccccatgcc ggatcgatat tttaagaccg gtaccaatct
     2761 ttattacggg atatcggccg cgcacggatt tatggtttcg acggacggcc gtggctgggt
     2821 agagagaaat cagggtttac ctcagaagaa tgtttatcct tttaaggagc cgcgtccccg
     2881 gccgttgacc gctttgggag tcgatccgct ggatccgtta aaggtggcgg tcaccacctc
     2941 tacccggctg tatttctccg agaacggcgg cgagagctgg cgggagattc cactgcatgc
     3001 cccgatctcg ggcggcacat acttgacttc cgtggcgctt acgaccgtcg caacgaaagt
     3061 acatatgctc attggaaccg cctttgccgg aatctatgaa agcatcaatc taggtcagag
     3121 ctggagttca atcacccccc gcctgcgttt tctatatcag ggagccggct tttgggagga
     3181 gatcgccgga atcgtatatt ctccggaaga gcccggggta atctatttcg cggccggctt
     3241 cgggcatggc ctataccgct gggaccgaac tgcgcaaaag gtgacccggc tcgattccgc
     3301 cacaccggtt acgatcaaca ctgcagtcga agaggcgttg aacccgggtg ttgcaactaa
     3361 ccccgaaggg tttcaaccgt tggttttaca ctggattccg gatccggagc cagttgcgcc
     3421 ggatttgaaa ccgaactgga tcctggagga ga
//
LOCUS       DASVMB010000483        10064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_205890,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000483 DASVMB010000000
VERSION     DASVMB010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205890"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VF531_13545"
     CDS             complement(<1..1020)
                     /locus_tag="VF531_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715032.1"
                     /translation="MRVGRLRSFKPFWLLLLLMSCSILACTVAAEEAGVDQSQPLVTA
                     TFFETDLREALHEIAIQTGINMTVDENVRGVVTLELKGVPFEKALKMILISGGFSFRK
                     VDDFYVIGWADPRNPGFAGLSESQVYYFKNISVDSAKALLPEFYKPYVKFGIGDKERQ
                     DVAMVSAPPDLLQAILADLKKMDGNREQIKIRALVTEVSNEVLKEWGMDLLKIDFNAV
                     KGSGLELLQLNIPEGTISGSGRVDFGKFETIIKALANEKKAVIHADPVLLVTEGESGE
                     LFIGEKRTLILHSQGTSSTTSSTENVEAGTALKVTPRLFDGQIELAVLERISGFDNST
                     TEEITV"
     gene            complement(1099..2625)
                     /locus_tag="VF531_13550"
     CDS             complement(1099..2625)
                     /locus_tag="VF531_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715033.1"
                     /translation="MRKKLIFIGLFLFMLTFAASNLMAIVVEPLTLNYNIKPGATARF
                     KITIKPEDKSSNLKVSLFRVTQRPDGGTDFLPADPETYPPSGWVSFPPTAKVNPGANT
                     ELSGTIKVPFGSKGTHNMMLMIESDQDKKTVQGLTIMLRYGVAINVRVDGPPIRATAE
                     LTRFGMAKTKKGLPLVMAEIKNTSAVDYPTSATATIRNSQKQLIERIELRPQSYWESG
                     GSEPVMYPESQLTYLGPINQYLAPGEYEVRLFYRYGDRGQILQAKTIKIREGDYSFPA
                     TKMQSLAVSPGELSFEGKPNAVSIKAFKLQNRTGEKVQVAIEPAEIEANYAYSIFANT
                     QVELKGDTRFTLEPKQMAVKIVSVRFPKDAPPQGNYGAYLVKVYSVAAKPVLIATEQI
                     RLAAEVNGAKKPGLTVTSISGDRNDTSCLIAAVIKNTGNIQVTPGVNLVLRDKEQKMV
                     GRIALVSENEGEASILPNKKATVTGVLRNIPPGKYHAEVQLTSGSDDLGTADLELEIK
                     "
     gene            complement(2797..3507)
                     /locus_tag="VF531_13555"
     CDS             complement(2797..3507)
                     /locus_tag="VF531_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715034.1"
                     /translation="MRKLILVMMAIGLIVAMSVSAFAYTELPITREAGHPDADGLQIA
                     KSGDFLYVNPFAAVKLVGITPAILEGDGYHLVSTGNVSFALACNSPVKLVVTASQFTA
                     KDDVLHPNHYRGEGDSYNIVTPLLASSANGTYANSVVLTKPNDGGSYPGPAAANYDEW
                     TYENQKVYFQVTAPVDRLNPYTLNNIDELHAGWYTGTIYFTVTQNGEAGEGTFDIIEP
                     VGGHQDLPDNFFDDYQAQ"
     gene            complement(3887..5068)
                     /locus_tag="VF531_13560"
     CDS             complement(3887..5068)
                     /locus_tag="VF531_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7715035.1"
                     /translation="MLRIFCLGAFRVLRPGEQEPIGVGSKHKIWMLFKYLLINRCQAV
                     TTERIIDCLWHSLKDPSDTAALRTTVSRLNSLLEPDRSSYGKATYIICRKESCFFNQE
                     ASCWIDAAYFEDLLRQAHQIGASDRTAGIERYRQALELYRGDFLSEDTDEEWTILTRE
                     YYRRLFMEAVHEAAGWLFELEEYEQARLILERAVRFDPYNEESQVLLIRIFLRLGEIE
                     AAAGHYSRISTILYRELGVRPSIKLKQVYKEIQSQRTAGSEMQWLEETLTSLEKPNGP
                     FVCEPEFFWNFLRFERRQLARSGGQSSLVILTLGSSNQAADRSGIEIIAGLVAVICQK
                     LRRCDLLCRLGAYQLAILLLFTDTAGGRIVINQISGTFYSQAGHSSYILETRVKPVLP
                     L"
     gene            complement(5441..5617)
                     /gene="rpsU"
                     /locus_tag="VF531_13565"
     CDS             complement(5441..5617)
                     /gene="rpsU"
                     /locus_tag="VF531_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7715036.1"
                     /translation="MAEVKIGKNETLDSALRRFKRQCQMSGILAEVRKREHYEKPSVR
                     RKKKSEAARKRKSS"
     gene            complement(5748..6092)
                     /locus_tag="VF531_13570"
     CDS             complement(5748..6092)
                     /locus_tag="VF531_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7715037.1"
                     /translation="MSDCLFCKIAGREIPSQIVLETPEVLIFKDIHPAAPLHLLAIPK
                     KHVDNICDPSLLDQDILRNIAAAIQETAAKLNLKENGFRVVVNYGHDAGEAVPHLHFH
                     ILAGRQLGWPPG"
     gene            complement(6184..7476)
                     /gene="mtaB"
                     /locus_tag="VF531_13575"
     CDS             complement(6184..7476)
                     /gene="mtaB"
                     /locus_tag="VF531_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX7715038.1"
                     /translation="MRVAVSTLGCKVNQNDTQSIISLFQQAGHEIVPFAEKADVYIIN
                     TCAVTRTSERKSAQAIRRAVAASPDAIVIGTGCYAQVAPEELAAIAGVNLVVGMVDRP
                     RLVELAEEFRHERKNRVAVGPVAESSFWVAPPAHIGLERTRATLKIEEGCEQFCSYCI
                     VPFARGPVRSMPPELVREEFLRLLELGYQEIVLTGIHLGCYGKDLGMNLARLLHQLLE
                     IKGHFRVRLGSLEPIDINPELSRVIIGNEKICQYLHIPLQSGCDSILAAMNRDYTSAY
                     YAELLDRLRNKNPYIGIGTDLIVGFPGETGADFEITRKFVAEQRFSRVHVFRYSPRRG
                     TPAADLPDRVAPAVQEERSSVIQTLSVSSGVEFARGFIGREVQVLFEESTDRGWSGWS
                     GEYLRVTVESDRQLKNCLVKVCATDLSGKDLAGCIPEQ"
     gene            complement(7481..8083)
                     /locus_tag="VF531_13580"
     CDS             complement(7481..8083)
                     /locus_tag="VF531_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715039.1"
                     /translation="MSLFSGSLTIIGWLAMGFGLQSLTRWSPWVNLAISLLGVFVLPP
                     FWSSGLVLGIWISCAFFTYHPELARDSIAGISWIRLIGYAALTFSGFLLTLILLYRLK
                     LDGSFGIGEREVVAWVFLGILEICLYKVIGRLAPGEYKMQYGYGIAFINFTMLLYWVI
                     PLGPYWMGLIFITLLLIDPILLSLVDRSGNSGNRTMMGSR"
     gene            complement(8161..9180)
                     /locus_tag="VF531_13585"
     CDS             complement(8161..9180)
                     /locus_tag="VF531_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7715040.1"
                     /translation="MELRYAVADEPFSGEFLIIANQLRTAKNGRSYLAMRIGDGTGEL
                     PVKIWDADVELFNSLEAGRVIALQNIRPRLFNHQIQLEWNGRDRELFRVLPEAETDYQ
                     KFLPQSPSDLNQCWDFLAEVIVTIDDPVLQMVLSYFFSNPDFKAAFIRVPAALKRHHA
                     YIGGLLEHTAGVTAICKAAAEYYPLVKRDLLLTGAILHDIGKVKTYQITKGFDGTTEG
                     KLIGHLVLGVEMVEEAFANLNKGYERGELHNRLIHLLVSHHGIMEWGSPVEPLTLEAC
                     ILHHADNLDAQVTKYLTVMRSEVQPAEWSAFDPGLGRSLFLGSAVEPEEESSTMAPES
                     SSGLE"
     gene            complement(9259..>10064)
                     /locus_tag="VF531_13590"
     CDS             complement(9259..>10064)
                     /locus_tag="VF531_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX7715041.1"
                     /translation="LQTQRMALCFPDPGRVRENLRLIYGIGETTAAKLHQEGITRLDE
                     LVDHPRWGKAATELLRVIAAGDIERLAGYGATDLELLGFYQPEALCFVDIETIGLYYV
                     QPVFLIGLLRFDNQCGVITQILARSFDEEKAILTAALAELKTASLIASFNGRSFDLPY
                     LKGRMRYHHLEHEWRTFHFDLLRPARRSYRGVLPDCRLLTLEREVLGEERGDDLPGSM
                     VAEYYHRFLDTGDRGWVEPILRHNAHDLLSIAKLLGLFTNRQQATGNRQ"
BASE COUNT         2571 a         2740 c         2236 g         2517 t
ORIGIN      
        1 gacggtaatt tcttccgtag tactattatc gaaaccgctg attcgctcca aaaccgccag
       61 ctcaatctgg ccgtcaaaga gacggggtgt gactttgaga gccgttccgg cttcaacgtt
      121 ttccgtactg gaagtggtgg agctggtacc ttgagaatgt aagatcagcg tccgtttttc
      181 gccgataaaa agctcaccgc tttccccttc ggtgacgagc aataccggat ccgcgtgaat
      241 aactgctttc ttctcattgg ctaaagcttt gatgatggtc tcaaattttc caaagtcaac
      301 ccgaccggaa ccgctgatcg taccctcggg aatattcagc tgcagtaatt ccaggcctga
      361 tcctttcacg gcgttgaaat cgatttttag taaatccata ccccattctt ttaagacctc
      421 attggaaacc tcggtgacca gcgctctgat tttgatctgt tccctattgc catccatttt
      481 tttgagatct gccagaatcg cctgaagcag atccgggggc gcgctgacca tcgccacatc
      541 ctggcgttct ttgtcaccga tcccgaattt cacataaggt ttgtagaact cgggtaagag
      601 cgccttggcc gaatcaacgc tgatattttt gaagtagtaa acctggctct cggatagtcc
      661 cgcaaaaccc ggattccgtg ggtcggccca gccgatcaca taaaagtcgt cgactttacg
      721 aaaactaaaa ccaccgctga ttaagatcat cttcaaggct ttttcaaacg gtacaccctt
      781 tagctccaag gtaacgactc cccggacatt ttcatcgaca gtcatattga tcccggtttg
      841 aatcgcgatt tcatgcagcg cttcccggag atcggtctca aagaaggtcg ccgtgaccaa
      901 cggctgagat tgatcgactc cggcttcttc ggctgcaacc gtgcaggcga gtatactaca
      961 ggacattagc aacaataaca gccaaaaagg cttgaaactc cttaaccttc caacccgcat
     1021 cgtggtacct cctactcctt ctctattttg gaacgacggc gtggtttcga tgatcagccg
     1081 atatatcggc acataacatc acttaatctc cagttcgaga tcggcggttc ccaaatcatc
     1141 gctcccggat gttaattgta cttcggcatg atatttaccg ggtggaatat tccttaacac
     1201 tccggtgaca gtggcctttt tattcggtaa aatagacgct tcaccttcat tttcactgac
     1261 aagcgcgatc ctaccgacca ttttttgctc tttatccctc agaaccaaat ttacccccgg
     1321 agttacctga atattcccgg tatttttgat gactgccgct atcagacagc tggtgtcatt
     1381 ccgatctccg gagatgctgg ttaccgtcag ccccggcttt ttggcgccat tcacctcagc
     1441 ggccaaacgg atctgttctg tagcgatcaa aacgggcttt gcagccacag aatagacctt
     1501 aaccagataa gctccataat tcccctgggg cggagcatcc ttaggaaatc tgacactgac
     1561 tatcttgacg gccatttgtt tcggttctaa tgtaaaccgg gtatcaccct tcaattccac
     1621 ctgagtgttg gcaaagatcg agtatgcata attcgcttca atctctgcgg gttcgattgc
     1681 cacttgtacc ttctcaccgg tccggttttg cagtttaaac gctttgatgg atacagcatt
     1741 cggtttgcct tcaaaagaga gttctcccgg actcaccgct aagctctgca tcttcgttgc
     1801 aggaaatgag taatcccctt cccgaatctt gattgtcttg gcctgaagta tctggccccg
     1861 atcgccgtaa cgataaaaca gtctgacctc atactctccg ggcgccaaat attggttaat
     1921 agggcccaaa taagtcaact gactctcagg gtacataacc ggttcgctgc ccccggactc
     1981 ccaataactt tggggtcgga gttctatacg ctcgatcaat tgtttctgac tgttgcgaat
     2041 ggtcgccgtg gcactggtcg gatagtcaac ggcagaagta tttttgatct cagccataac
     2101 taagggcaaa ccttttttgg tcttggccat tccaaatcgt gtcagttcag cggtagcgcg
     2161 gattggtggc ccatcaaccc gcacgttgat ggcaacccca tagcgaagca tgatcgtcaa
     2221 tccctggacc gtctttttat cctgatccga ttcgatcatt aacatcatat tgtgagtccc
     2281 cttagatccg aagggaacct tgatggttcc ggaaagctct gtattcgccc cggggttaac
     2341 tttcgccgta ggcgggaagc tgacccaacc ggatggagga taggtttcgg gatccgccgg
     2401 cagaaagtcg gtccctccat cggggcgttg tgtcacccga aacaaagata ctttgagatt
     2461 acttgattta tcttcgggtt tgatcgtaat cttgaaacgt gcagtcgctc ccggtttgat
     2521 attataattt aaggtgagcg gctccacgac aattgccata aggttagatg ccgcgaacgt
     2581 cagcataaac aaaaaaagtc caataaagat taactttttg cgcatttatc aatccacccc
     2641 ttcatcctgg aaggtacagc cgtttttacg gtttcgattc gtataatggt ctcttgttta
     2701 attgccttta tcgtttcttt caccagggaa cgatagactt acagccagga gcgggctcgc
     2761 aagagcccgc tgcctgacca atcaaataaa tcttgattat tgagcttgat aatcgtcgaa
     2821 gaagttgtcg ggaagatcct gatggccgcc aaccggttcg atgatatcga atgtgccttc
     2881 gccagcctcg ccgttctggg tgacggtgaa gtagatggtg cccgtatacc aaccggcgtg
     2941 aagttcatcg atgttattca atgtataggg gttaagccgg tcaaccggag cggttacctg
     3001 gaaatacacc ttttgattct cataggtcca ttcgtcgtaa ttggcagccg ccgggccggg
     3061 gtaggagccg ccgtcgttcg gtttggtcaa taccacggag tttgcataag taccgtttgc
     3121 actgctggcc aaaagcgggg tcacgatatt gtagctgtcg ccctcgccgc ggtaatgatt
     3181 gggatgcagc acgtcatctt tcgcagtgaa ctgggaagcg gtgacaacca atttcaccgg
     3241 ggagttgcaa gccagcgcga aagagacatt gccggtggat accagatggt aaccgtcgcc
     3301 ttccagaata gccggtgtaa tccctacaag ctttacggca gcgaatgggt taacatacag
     3361 gaaatcgccg gatttagcga tttgcagacc atcagcatcc ggatgacccg cttctctggt
     3421 aatcggtagc tcggtgtagg cgaaagcact aacactcatg gctacgatca aaccgatagc
     3481 catcattaca agaatcagtt ttctcattcg aatggttcct cctcaagttt ttaatttgcc
     3541 aagatcaccc atctggttgg gtcatgtttt tccccatggc ctgctctctc ttgaaacgtc
     3601 taatgcaacg aacaagacaa atggccgacc ctgatcaccc cctccggatc tttttccgct
     3661 tccgccggtt aggagaaatt gaactacgtt tgaccatgcc aaggcggaaa accggaaagg
     3721 cacgccggaa taacggacgt gcttcgaact gtagtcattc tatgcgccgc aatccctttt
     3781 tatactaaaa tctccaatat atcaaaatag gatcgcgccc ttcctattac gatcttagct
     3841 gagacttgtt actggtttgt tactgtacgg ccggaacaaa tcttcgtcat aatggcaaca
     3901 ccggttttac ccgagtttcc aaaatatatg acgaatgacc cgcttgacta tagaaggtcc
     3961 cgctgatttg attgataaca atccgaccac ccgccgtatc ggtaaacaat aataatatcg
     4021 ccaattgata agcgccaagc cgacatagta gatcgcatct ccttagtttt tggcaaatca
     4081 ccgccaccag gcctgcgata atctctattc cactcctgtc cgcggcttgg tttgaactac
     4141 cgagcgtcag gatgaccaaa ctggactggc cgccgcttcg cgccaactga cggcgttcaa
     4201 aacgtaaaaa attccaaaag aactctggtt cgcatacaaa aggaccattg ggcttctcga
     4261 ggctggtcaa agtctcttcc aaccattgca tctcggagcc tgccgtcctt tgagattgaa
     4321 tctccttgta aacttgtttc aattttattg atggccgaac gccgagttcc cggtagagta
     4381 tggttgagat ccgggagtaa tgaccggctg cagcttctat ttcccctaac cgcaggaaaa
     4441 tccggatcaa aagaacctgt gattcttcgt tgtaggggtc aaaacgcacc gcccgctcca
     4501 gaattaaccg ggcttgctca tattcctcaa gttcaaacag ccaacccgcc gcctcatgga
     4561 cagcctccat aaacaatcgc cggtaatact cccgggtaag tatagtccat tcctcatccg
     4621 tgtcctcact aaggaaatcg cctcgataca gttcaagcgc ttgccggtat cgctcaattc
     4681 ctgccgtacg atcactggcc ccgatttgat gggcctgccg caaaaggtct tcaaagtatg
     4741 ccgcatctat ccagcatgaa gcctcctgat taaagaagca ggattctttt cgacaaatga
     4801 tataagttgc cttgccatac gaggagcgat ccggttcaag caatgagttg agtcgggaga
     4861 ccgtggtccg tagggctgcg gtatcactgg gatcttttaa ggaatgccac agacagtcga
     4921 taatccgttc ggtggtaaca gcctggcatc ggttgatcag caaatattta aagagcatcc
     4981 agattttatg cttgcttcca acaccgattg gttcttgttc cccgggccgg agcacccgaa
     5041 aagcgcccag gcaaaagatt cggagcatct gttctcttcc ctgaccggcc gattgattcg
     5101 gatattcgat gacgttgccc ccctcccgac cacacaattc atgacccgca aaacagagta
     5161 ttcaagtaaa gaacaatagt ttcgcgtaag ccactgtttt ttaagatacc atatcttgga
     5221 atcatatcgt gtcggtttat gaaacggaat accggttatt ttcgagaatt atccggaaga
     5281 attatgtagg acggggtaaa aaaaaaccgg agtcgcctcc ggtttaaaga tgaatacgct
     5341 atttatagaa acagctgaat tccaaaaatg aaagcaaata cggcatgtgc aggacgagcg
     5401 gtacccgaca aaaaaaggcc cgtcatcgac gggccttttt ttaagaggac ttacgtttcc
     5461 gtgctgcctc tgactttttc ttcctgcgta cactcggctt ttcgtaatgc tcccgcttgc
     5521 gaacttcggc caaaatgcca gacatctggc actgcctctt aaaacggcgc aacgcgctat
     5581 ctaaagtctc atttttacca atcttaacct cagccacata tttccctccc ctccactgct
     5641 tgcctacctc gcaaccccgt ccgcagtgtc cggtttgaag ataaactaat ccaaaagctt
     5701 aatcacatta taatattata ctacaatatg cgaaatgctg tcaacaatca gcccggcggc
     5761 caacccaatt gacggccggc caatatatga aaatgcaaat gcggtacagc ttcacccgca
     5821 tcgtgaccgt aattgactac cacccgaaaa ccgttttctt tcagattcaa ctttgccgct
     5881 gtttcctgga tggccgcagc gatgttccga agaatatcct gatcaagtag actcggatcg
     5941 cagatattat cgacgtgctt cttgggaatc gccaacaaat gaaggggagc ggccggatga
     6001 atatctttaa agatcaaaac ctccggagtt tccagtacga tttggctggg aatctcacgg
     6061 ccagcgatct tacaaaacaa acaatcagac atttacctct tcctttcatc ccttccagat
     6121 aaccgaacac ccattcacaa atatttgttt cgacaaggat tctataaatt ccttcaaaat
     6181 atttcattgt tccggaatac atcctgccaa atctttcccg gataggtcgg tggcacagac
     6241 cttgaccagg caattcttca attgccggtc ggattcgaca gtaactcgga ggtattcacc
     6301 cgaccaaccc gaccaacccc ggtctgtaga ttcttcgaat aagacctgta cctcccgacc
     6361 gataaaaccc cgggcgaact ctacgccgga gctgacagag agggtctgaa tcacactact
     6421 ccgttcctcc tgcactgccg gcgcaacccg atccggcaaa tccgcagccg gagtgccgcg
     6481 tcgtggcgaa taacgaaaaa catgaactct gctgaaacgc tgctcggcca caaatttacg
     6541 ggttatctca aaatccgccc cggtctcgcc gggaaaaccc acaatcaaat cggtgccaat
     6601 accaatatat ggattcttat tccgtaaccg gtctaaaagt tcggcgtaat atgcactggt
     6661 ataatcccga ttcatcgcag ccagtatgga atcgcacccg ctttgcaagg ggatatgcag
     6721 gtattgacag attttctcat ttccaatgat caccctggat agctcgggat tgatatcgat
     6781 tggttccagc gaacccaatc gaaccctaaa atggcctttg atctcaagca attgatgtaa
     6841 cagccgggca aggttcatcc ccagatcctt accgtagcaa cccagatgaa ttccggtcaa
     6901 tactatctcc tggtaaccaa gttctaaaag gcgaagaaat tcctccctga ccagttccgg
     6961 aggcatacta cgtaccggcc ctctggcaaa tggcacgata cagtacgagc aaaactgctc
     7021 acagccttcc tctatcttaa gcgtagccct ggtcctttcg agtccgatat gagcgggtgg
     7081 tgccacccaa aacgaactct ccgccaccgg gccgactgca acgcgattct ttcgctcatg
     7141 ccggaactcc tcggctagtt ccactaaacg gggtcgatcc accatcccga ctaccaagtt
     7201 tacaccggcg atcgctgcaa gctcttccgg agcaacttgg gcgtaacagc cggttccgat
     7261 gacaatggca tccggagaag cggcgacagc ccggcgaatt gcctgagccg actttcgttc
     7321 actggtccgt gtcacggcac aggtattgat gatataaaca tcggcctttt ctgcaaaagg
     7381 aacgatttca tgacctgctt gctgaaataa actgatgatg ctctgtgtat cgttctgatt
     7441 gaccttgcat cctaacgtac tgacggctac tctcatcaat ttatctgctt cccatcatgg
     7501 ttctgttacc actgttgccg cttcggtcga ccagactaag caggatggga tcgatgagca
     7561 gtagagttat aaagatcaag cccatccaat acgggccgag gggaatgacc caatacagga
     7621 gcatggtgaa gttgataaac gcgatcccgt aaccgtattg catcttatat tcccccggag
     7681 ccaaccggcc gatcacctta tataaacaga tctcgagaat ccccaaaaat acccaggcca
     7741 caacttctct ttcccctatt ccgaaactac cgtcaagttt aagccggtat agcaatatca
     7801 acgttaaaag aaatcccgag aaggtgagcg cggcgtagcc aattagccga atccagctaa
     7861 ttccggcaat cgaatcacgc gccaattccg gatgataggt aaagaaggcg cagctgatcc
     7921 agattcccaa gactaaacca ctgctccaaa aggggggcaa aacgaaaacc cctaaaagtg
     7981 agatggccag gttcacccaa ggagaccatc gggtcaagga ttgtaacccg aaacccatcg
     8041 ccaaccaacc gataattgtt aagcttcccg aaaataaact catctaatca ttctcctcct
     8101 gcaatacccg tttcattaaa gagcactttc tggtatacat tcgtttccaa gccccggaac
     8161 ctactccaac ccggaacttg attcgggcgc catggtactt gattcttctt ccggttctac
     8221 cgcactcccg agaaacagtg aacgccccaa gccaggatca aatgcgctcc attcagcggg
     8281 ttgaacctca ctacgcatga cggtcagata cttggtgacc tgtgcatcca ggttgtcggc
     8341 gtgatgcaaa atgcaggcct ccagagttag tggttccacg ggtgatcccc attccatgat
     8401 cccgtgatgg ctaacgagaa gatggattag acggttatga agttcccccc gttcgtaacc
     8461 cttattaagg ttggcaaacg cctcttccac catttccaca cctaacacca aatggccgat
     8521 aagttttcct tccgttgtac cgtcgaatcc cttcgtaatc tgatatgttt tgactttacc
     8581 gatatcatga agaattgcac cggtcaataa aagatcccgt tttaccagag gataatactc
     8641 agccgccgcc ttgcagatcg ccgtgactcc cgcagtatgc tccagtagcc cgccgatata
     8701 ggcgtgatga cgtttaagtg cagcaggaac ccgaataaac gccgccttaa aatccggatt
     8761 ggagaagaaa taactcaaaa ccatttgtaa aaccgggtca tcaatggtaa caatcacttc
     8821 cgctaaaaaa tcccagcact gattgaggtc gctgggagat tgcggcagaa acttttgata
     8881 atccgtctcc gcttcgggca gtacccggaa aagctccctg tccctaccgt tccattccaa
     8941 ttgaatctgg tggttaaaaa gccggggccg gatattctga agtgcaatca cccttcccgc
     9001 ttccaggctg ttaaagagtt cgacatcggc atcccagatc ttgaccggca actccccggt
     9061 gccgtcgccg atcctcatgg ccaagtagga tcggccgttt ttagccgtac gtaattggtt
     9121 ggcgataatc aaaaattcac cgctaaacgg ttcatccgca acggcatatc tcaactccat
     9181 caatggcacc tcccgaataa agtgtgaagt ttgaagggtg aggggtgcgc ctcttgcctc
     9241 ttgcctcacg cctcacgcct attgcctgtt ccctgttgcc tgttgcctgt tcgtgaacaa
     9301 ccccaacaat tttgctattg aaagcaagtc atgggcattg tgtcgcagaa tcggctcaac
     9361 ccagccgcgg tccccggtat ctaaaaacct atggtaatac tcggcaacca tacttccagg
     9421 aagatcgtcg ccgcgctctt cccctagcac ttcccgttcc agggtaagca gacggcaatc
     9481 gggcaacacc ccccggtagc tccggcgggc cggacgaagc agatcgaaat gaaaagtccg
     9541 ccattcatgc tccaaatgat gatacctcat ccgccctttt agataaggca gatcaaagct
     9601 gcgtccgtta aaactggcaa tcagggacgc cgtctttaac tcggctaacg ccgcagttaa
     9661 aattgctttc tcctcatcga aactcctcgc caaaatctgg gtgatcaccc cgcattgatt
     9721 atcaaaacgt aacaatccga tgaggaatac cggttgtacg tagtaaagcc cgatggtttc
     9781 aatatccacg aaacaaagcg cttccggctg ataaaaaccg agcagctcca gatcggtggc
     9841 accatatccg gccaaccgct cgatatctcc cgcagcaatc acccggagaa gttccgtggc
     9901 tgccttgccc caacgaggat gatccaccag ctcatcgagt cgggtaatcc cctcctggtg
     9961 aagtttggct gcggtagttt ccccgatccc atagatcaga cgcaaattct cccggactcg
    10021 tcccggatcg ggaaagcaaa gggccattct ttgagtttga agcg
//
LOCUS       DASVMB010000484        10521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_355419,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000484 DASVMB010000000
VERSION     DASVMB010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355419"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            653..1033
                     /locus_tag="VF531_13595"
     CDS             653..1033
                     /locus_tag="VF531_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715042.1"
                     /translation="MSIIWDEKNPSDMNSDEIIKKLIDIYSEYQQELAATSSPGNGSI
                     FIGMATENDTELIKGLLNLPSELRYKLWDFICANSIPTKPGTIPRSTDSKDTNTTKEQ
                     SIKELLLNSPHKEIEILIDHPAEK"
     gene            complement(1459..1647)
                     /locus_tag="VF531_13600"
     CDS             complement(1459..1647)
                     /locus_tag="VF531_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715043.1"
                     /translation="MLTKYHALQSENNHLKDEIKRLKLQLGIPEQPGIPFDFSGQEPT
                     PEMIKADCNEVETSDIND"
     gene            complement(1838..2149)
                     /locus_tag="VF531_13605"
     CDS             complement(1838..2149)
                     /locus_tag="VF531_13605"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7715044.1"
                     /translation="MQNVLNDAINIIVKNFAPDKIILFGSHATGMASRDSDYDLCILK
                     SDLQHKRKTAQDVYKALVGLQFPVDVIVETPEKFEELKTNPFLIYGKIAENGEILYEK
                     Q"
     gene            complement(2276..2755)
                     /gene="rlmH"
                     /locus_tag="VF531_13610"
     CDS             complement(2276..2755)
                     /gene="rlmH"
                     /locus_tag="VF531_13610"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX7715045.1"
                     /translation="MNRIRIIAVGKLKEEFYRAAQEEYLKRLRPYLKLEIVETSDLPC
                     PERSTPAQEEQVLKQEGALIRSKLQPGDFVVVLDRKGKEIDSLEFAGLVQERLASGKP
                     LAFVIGGSLGVDPELLKEAQMRLSFSKLTFPHQLFRVVLLEQIYRAMKINRGERYHK"
     gene            complement(2755..3975)
                     /locus_tag="VF531_13615"
     CDS             complement(2755..3975)
                     /locus_tag="VF531_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7715046.1"
                     /translation="MNEFRYDAHEPRRIIWIVAIISGIIGALLVVIAVRFTGLGTALV
                     SPPVAQELPEPPPTQMQPVVNNASPLSMEHQTIDVVKQVGQAVVMITTSSVVQSFDFF
                     TGLESRSIQGLGSGVVYSSNGYILTNNHVVNGLPGTKDKIMVILFNKEYPKGKTYPAR
                     VVGADPRTDLAVLKIDVTNLPVPQWGDSNQVQVGQTAIAIGNPLTKSLNNTVTVGVVS
                     AKERTLEVNNNEQLLNMIQTDASINPGNSGGPLLDSNGRIIGINTAIAPNSQGIGFAI
                     PSNTAKTVAQQLISRGYVSYPGVGLVYIPLDADTVAQLEGNLGVRLPVNKGLFIYKVI
                     QGGPAALAGMRPGDIIVKLNGEKITDQDLVREAVARHRIGDSLTLEYYRGNSIRKSTV
                     RIAELGRRIGELSR"
     gene            complement(4094..4870)
                     /locus_tag="VF531_13620"
     CDS             complement(4094..4870)
                     /locus_tag="VF531_13620"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715047.1"
                     /translation="MDTYGDNSGSRMGAPLNSGKGQGGDGGAVSWKTLQTRLLSNYEL
                     REVQLTLEGHSLKLTKVADIDALLDKVSDADRIPFWAELWPASIGLARFILQRERWFS
                     GHTLLELGSGIGLAGIAAKLAGASVVQSDFLEEAFTFIRLNCIRNGVEPDPLLLADWR
                     SFPQHAGGFDWVIGADILYEKTLHDSLEKVLHQVVKVGGGVLLADPGRGYAAQFIERL
                     IAAGWQCRKLQLPVVYEERNYQIDIYQLQPKGTGDSLISL"
     gene            5073..5456
                     /locus_tag="VF531_13625"
     CDS             5073..5456
                     /locus_tag="VF531_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715048.1"
                     /translation="MSHHNNSNNPETKEKTESISAVTAESPVHPFLSKDDMKLLSIMK
                     PFLSTNGQKMVGFFLSFGDETPGDSSPDFSNLLRQAVNKGDAGPLQELLPVLLSSMAG
                     NSNKGGLNPALLTTMMSMLNAKKNT"
     gene            complement(5597..6160)
                     /gene="spoVT"
                     /locus_tag="VF531_13630"
     CDS             complement(5597..6160)
                     /gene="spoVT"
                     /locus_tag="VF531_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HEX7715049.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFVDREGEV
                     ILKKYSPIGELGDFAKEYADSLYESTGHIACIADRDMVIAVAGAAKKEFLDKPISKAV
                     EKAIEDRKVVLMPKPGEHKYCEACPVTEDEGKCKFTAQVIAPIIAEGDPIGAVILSSK
                     EPGTTMTDLEVKIAETAAGFLAKQMEQ"
     gene            6645..8624
                     /locus_tag="VF531_13635"
     CDS             6645..8624
                     /locus_tag="VF531_13635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7715050.1"
                     /translation="MRKWPFTVLLSLCLFIPLAFAVPAFDRFNTPKLVLFLFGSGLLL
                     IWELKNFQGFRELPVSVKWAAGFFLAVELFQMVRLADWSTGLIGKYGQSESFLVQAGF
                     LVMGAAAYRFTAGEAERSRLRRVLMLVTFFICCFGIGEYFGGDPIARQDVTRVKSFFG
                     DPNSLGAFLILTLPLIFKEWQETPKRSVRYFAAGCILLGSATLILTFSRASWLGFLVG
                     LALFILFQAVGLTPKKLLKIIVIATVIWGISWATGSVWVGGALFYLLILLGMAWNTAA
                     RRPWVIGLVILVILGATIGQTLTYFQPRQHSDYELKARVTSIAQGNDSGRGLLWSIGW
                     RIFQESPWIGHGLGSFRDNFHRFETQAAVKFWGPDRDLDQSHNEPLHYLATQGILGFL
                     AYFGLLLAILASTDWRGLLRRNLSLSETSLFCACLSYMIFVNFGYALVHYTFIFWIEL
                     GLLLGYRHPAPQIRTSPTLIPTWLLPLALWIFIIGGGWILGNMFLADLNYRTAFHQAR
                     RHQFEESLGSYRLVIARAPLIEHYHYRYALTLFRAAKWYQKQGGDSETADQYSRQSRA
                     ILLRLRRIHPDEYQLNFLLGQEYENGSEFQEAAAYYRQALIQFPENYRLYIRLAKAEW
                     LGGNKLASRRAFDAGFAMNPEYAQKAAIAEGLPLP"
     gene            complement(8629..>10521)
                     /gene="mfd"
                     /locus_tag="VF531_13640"
     CDS             complement(8629..>10521)
                     /gene="mfd"
                     /locus_tag="VF531_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7715051.1"
                     /translation="RLEYAGEDRLFVPTDQVNLLQKYIGVEDAPPKLNKLGGADWQKV
                     KNRVKESIRDMAEELLDLYARREVAPGHTFASDTVWQHEFEESFFFDETPDQLRAIEE
                     IKRDMESDKPMDRLICGDVGYGKTEVAMRAAFKAIMDGKQVGVLVPTTILAQQHYLTF
                     RERLTGFPINLRALSRFQTAQDQEETIAGLLSGEVDLVIGTHRLLSTDVHFKDLGLLI
                     VDEEQRFGVAHKERLKELRHNVDVLTLTATPIPRTLHMSLAGIRDISIIETAPQGRYP
                     IRTHVLEYNEAVIREAIQRELDRGGQVYFVYNRVETIERMASYLQNLLPNARITIAHG
                     QMDEDQLERVMLDFYDGQADVLVCTTIIETGLDIPNVNTLIVYDAERFGLAQLYQLRG
                     RVGRSNRLAHAFFCYRKDKVLSENAEKRLAAIREFTELGAGFKIAMRDLEIRGAGNLL
                     GPEQHGQIAAVGFELYCRLLDEAIRERKGEVQPELPEPVVEVPADAYIPGDYIADNKQ
                     KVEVYKKIAAAGTVEDVDLITEEIIDRFGEPPQPVVNLLNITKIKALAKRIGLASLTK
                     ERNELIAKLHIGLGMDYDKVLAVLQKYRNHFRYQPGRPPVFRWRTGLPEPELVKMVLH
                     TLEMMV"
BASE COUNT         2543 a         2768 c         2357 g         2853 t
ORIGIN      
        1 aaatcgtatt cttgactatt ggttattctc cccgcccgat tgacctttat cttaattact
       61 gaaagtcgac tttcccttaa ttcttttaag agaatatcat actcttgcct attgaggaga
      121 tcatcgtaag cggaagtaat ctggttgagg atcggttgca atttttccag ctcaaaccta
      181 actgatgcta atctccttaa ttgctgcaat tggaatatta acatctctag cgtggtaatt
      241 cccaaagaaa cttgattcga aagatgccta ataaatgaat taaatcgagg ctggtgtaac
      301 gctacctctt gacgatcctt ttcatatata agttggaccc gtttactgat tcccctatcc
      361 tgccggcaat acttcgctaa gacatcaacc aatttatgat tgggaactcc ggcaatagat
      421 attcctccct ccgtcgcatt aatgattttg acgtttgttc ggtattcacc gatgattctt
      481 actgaatttc atgaaagcat ggttttcatc aatcccacca aaatataatc ataccatcag
      541 gagggggcgg taagacttac cggatatatt tcaaaaaatt tggacatatt atccaatata
      601 tcttgtcaca tgattatcga caatgtagaa tgggggtaag gaagtgatca agttgagcat
      661 tatctgggat gagaaaaacc cgtccgatat gaactccgac gaaatcataa agaaactaat
      721 cgatatctat tccgaatacc aacaagagct tgccgccacc tcgtcaccgg gaaacggaag
      781 catatttatt gggatggcta cggagaacga taccgaatta ataaaaggtt tactcaatct
      841 accatcagaa ttacgataca aattatggga ctttatttgc gcaaattcca tacccactaa
      901 acctggtaca ataccccgaa gtactgattc caaagataca aacacgacta aagaacaaag
      961 tatcaaagaa ttactcctta attcgccgca caaagagatt gaaattttaa ttgaccatcc
     1021 cgctgaaaaa taagggaaat ccgttaccgg taatcgatgg tttactcgct gcaactgccc
     1081 aagacacatg atcttgttta cccgcaataa taaggatttt gccacaaccg gaattatcat
     1141 gcataatcct tggaacgaat agattcaact tgatccttag ttgtggtatt tttatccgta
     1201 tcgggtctaa tggccaaatt ttatgttctc cctttcttca aaaaactata ttaacgaatc
     1261 atctttcctt ttctacaatt ttgatgagtt cattggctag gtttagccgc ataatctggg
     1321 ctgccattca ttcaaacaaa ccggaagttc ccttcttttg cgccttcaag aaggcgagtt
     1381 ctcccacctt tttgcataga catcgtctag gcctctaaag agtgacatga ataattcgat
     1441 tttttccgcg ggggttgact aatcattaat atctgaagtc tcaacttcat tacaatctgc
     1501 tttaatcatt tccggcgtcg gttcttgtcc agaaaaatca aaaggaatcc ctggctgttc
     1561 cggaattccc aattgaagct tcagtcgttt aatttcatct ttaagatgat tattctcact
     1621 ctgtaatgca tggtactttg taagcaaatc cgttataatc cggtctttta ttttttagtt
     1681 gatctttcac ccattgtatt actttgcttg cgatgtctac agcttcgaaa tattcaacct
     1741 cagtcacagg ctcatattca ccgggatacc gcgtttcgac cgcataatca tttaatataa
     1801 cttccgaagg tacttgaata caccccctta aatacactca ttgtttttca tagagaattt
     1861 ctccattttc ggcaatcttc ccataaatta aaaatgggtt tgtttttaat tcctcaaatt
     1921 tttcaggggt ttctacaatg acatcaacgg ggaattgaag cccaactaat gccttataga
     1981 catcttgggc agtttttcgt ttatgctgca aatcgctttt taaaatgcat aaatcataat
     2041 cgctatcccg actggccatt ccggtggcgt gagagccgaa taagataatc ttatccggag
     2101 caaaattttt gacaataata ttgattgcgt cgtttaagac gttttgcatt ttgccaccgc
     2161 ctctttttat atttattata ccaaaattcg ttgcctcaag ccagttttca agtagaacac
     2221 ttatacttat cgccgttaaa ggtgatacac taaccgtatc tgttctaacg gcgaattatt
     2281 tgtggtagcg ctccccccgg ttgatcttca tcgcccggta gatctgctcc aataaaacca
     2341 ccctaaaaag ctgatgggga aaggttaact tcgagaagct caaccgcatt tgggcctctt
     2401 tcaacagttc cggatcgact cccagggaac cgccgatgac gaaggccagc ggctttccgc
     2461 tggccagtcg ctcctgaacc aacccggcaa actccagtga atcgatctct ttgcctttac
     2521 ggtcgaggac gaccacgaaa tccccgggtt gcagcttgga gcgtatcaag gcaccctcct
     2581 gctttaatac ctgttcctcc tgggccgggg tagaccgttc cgggcaaggg agatcgcttg
     2641 tctcaactat ctccagtttc aaataaggtc ggagccgttt gagatactct tcctgggcag
     2701 cccggtaaaa ctcttccttc aactttccca cagcgatgat ccggatccga ttcattaccg
     2761 actcaactct ccaatccgtc tacccaattc ggcgatgcgg accgtactct tacggatact
     2821 attaccccgg taatattcca gcgtcagact gtcgccaatt cggtgccggg ccacggcctc
     2881 gcggaccagg tcctgatcgg tgatcttttc cccatttaat ttgacgatga tatctccggg
     2941 gcgcatcccg gccagcgcgg ccggacctcc ctgaatcact ttatagatga acagaccctt
     3001 attcaccgga agccgtaccc ccaggttacc ctcaagctga gccacggtgt ccgcatccag
     3061 cggaatatag accaaaccaa ccccgggata ggaaacatag ccgcggctga tcaactgttg
     3121 ggccacggtc ttcgccgtat tgctgggaat agcgaatccg attccctggg agttgggggc
     3181 gatggcggta ttgatcccga tgatccggcc gtttgagtca aggagcgggc cgccggagtt
     3241 tccgggattg atcgaagcgt cggtctgaat catattcagt aactgttcgt tattattgac
     3301 ctccagggtt cgttccttgg cgctgaccac tccgacggtg accgtattat tcagactctt
     3361 ggtcaagggg ttgccgatgg caatagcggt ttggcctacc tgaacctggt tggaatcacc
     3421 ccactggggt accggaagat tggtaacgtc gatttttaaa accgccaaat cggtccgtgg
     3481 gtcagccccg actacccttg ccggatacgt cttgcccttg gggtattcct tattgaacag
     3541 aatcaccatg atcttgtcct tggttccggg caatccgttc accacatgat tattggtcag
     3601 aatatacccg ttggaactat aaaccactcc ggagccaagc ccttgaatgc tacgagattc
     3661 caatccggtg aagaagtcga aactctgtac caccgaactg gtggtaatca tcaccaccgc
     3721 ttggccaacc tgctttacca cgtcgatagt ctggtgttcc atactcaagg ggctggcatt
     3781 attgaccacc ggttgcatct gagttggcgg tggttcaggc agctcctggg ctacaggcgg
     3841 actcaccagg gccgtcccca gaccggtgaa tcttactgca atcaccacca acaacgctcc
     3901 gatgatgccg gagatgatcg cgacgatcca tatgattcgc cggggttcat gtgcatcata
     3961 acgaaattcg ttcactgcga ttctcctttt ttatcggccc aataccaagc gatgtgtgta
     4021 cgaacgctcg attaaacaaa aggcaactta atataagtta tcaggccggg ccttatcagt
     4081 tttcttttaa aaattataac gaaatcaatg aatctccggt tcccttgggc tgcaactggt
     4141 aaatatctat ttgatagttg cgttcttcat aaactaccgg taattggagt ttccggcatt
     4201 gccatcccgc tgcaatcaac cgttcaatga actgggcggc atatcccctg cccgggtcag
     4261 ccagtaacac acctccaccg accttgacga cctgatgaag caccttctct aaactgtcat
     4321 gcagtgtttt ctcatacagg atatcggcgc caatcaccca atcaaaccct cctgcatgct
     4381 gaggaaaact tcgccaatcg gccagtaata acggatcggg ttctaccccg ttacgtatac
     4441 aattaagccg gataaaggta aaagcctctt caagaaaatc ggattgaact acactggctc
     4501 ccgccagttt ggcggcgatg cccgccaggc cgatgcccga ccctaactct aaaagcgtat
     4561 gtcctgaaaa ccaccgctct ctttgcagga tgaaacgggc taaaccaatg gatgccggcc
     4621 agagttcagc ccagaatgga atccggtccg catcgctgac cttgtccaac agggcatcga
     4681 tatccgccac ttttgtcaat ttcaggctgt gtccttcgag ggtgagttgc acctcacgca
     4741 gctcgtagtt ggacaaaagc cgggtctgaa gagtcttcca ggataccgcc cctccgtcgc
     4801 caccttggcc tttacctgag tttagcggag cacccattcg cgatccgctg ttatctccgt
     4861 atgtatccaa cttttttcct cccactccat cccgccaaaa tccaaatggt aatcttcggc
     4921 atcccggcgt tttctccttt aattcccgct caaccgcgcc gcaagattcc ggttcccaat
     4981 catatcaacg tgacataaat tattatgggc ggcaaccccc gggtccggcg ataccctctt
     5041 ttttaccgga ccggagaagt ggaggaaaag taatgagcca tcacaacaac agtaataacc
     5101 cggaaaccaa ggaaaagacc gaatccattt cggcagtaac ggcagaatca ccggtacatc
     5161 cgtttttgtc caaggacgat atgaagctgc tatccatcat gaaacccttt ctcagcacaa
     5221 acgggcagaa aatggtcggt ttctttctga gtttcggtga tgaaaccccc ggggattcat
     5281 cccccgattt ttcgaatctg ttacggcagg ctgtaaataa aggcgatgcc gggccgctcc
     5341 aggagttact cccggtgctt ttgagcagta tggcgggaaa cagtaacaaa ggcgggctga
     5401 accctgcgct attaaccacg atgatgtcca tgttaaacgc gaaaaaaaac acctgaccag
     5461 acgttttccc ggacatggtt tggccccgcc agaccaatcc gtcctgagca gggccaacct
     5521 aagaacggcc atataaaaaa agtgtcggtt gaacccgtca cttttttttc gatcaccggt
     5581 actggtaact tccttgttat tgttccatct gtttggcaag gaaacccgca gccgtttccg
     5641 caattttgac ctctagatca gtcatggtcg tcccgggctc ttttgaggag agtattaccg
     5701 ctccaatcgg atcaccctcg gcaatgatgg gtgcgataac ctgcgctgta aacttgcact
     5761 ttccctcatc ctctgtaacg ggacaagctt cacaatattt atgttcgccc ggtttgggca
     5821 tcaggacaac ttttcggtcc tcgatggctt tctccaccgc cttggagatc ggtttatcca
     5881 aaaactcctt ttttgcggcg ccagctacgg caatgaccat gtcacgatcc gcaatacaag
     5941 ctatatgtcc ggtggactca taaagcgaat ccgcatattc tttggcaaag tcacccaatt
     6001 ccccgatggg agagtatttc tttaaaataa cctctccttc acggtcgacg aagatctcga
     6061 gcggatcacc ctctcgaatc cgtaatgttc tacggatttc ctttggaatg accacccggc
     6121 ccagatcatc aatccgtcgt acaattccgg tcgccttcat cccccatgtc cctccttttt
     6181 cacgcgttgc tgcgcagtat ttcggtatga agcagatctt tggtatcagg ggccttccaa
     6241 taagttttct atcaccatat ttggaaggtg ttcgatggct caccgtcaga tagacttgaa
     6301 acgtggatca tgccgcgatc ttcagtgata gtatataact cggtgaattt ttttattcat
     6361 ttttgcccga accaacgcgg gatcagttcg ccggtaaagc gcgcactcgt gggcgactta
     6421 ccctatctga ctggccgttt atagcttctc cgttttttct caatccatac caaggaaatt
     6481 tcccaaccca aagagaacca tcgattaccg gttcgactat ataccgccca cttaacccgt
     6541 tatcgggaaa ataaaaggaa tttctggaat cgcggataat tcttaggcat tgtcagatgt
     6601 ttgaacctta tgcctttggc ttatatccgg cggaggtgtt tcctttgcga aaatggcctt
     6661 ttacagtttt attgtccctg tgtctattca tccccctggc tttcgcggtt ccggccttcg
     6721 atcggttcaa tactcctaaa ctagtacttt ttctgttcgg ctccggtcta ttactgatct
     6781 gggagttaaa aaactttcaa ggatttcggg agcttcccgt cagtgtgaag tgggcagccg
     6841 gtttttttct cgcagttgaa ctcttccaga tggtacgatt ggcggattgg tccaccggac
     6901 ttatcggcaa atacggccaa tcggagtcat tcttggtgca agcgggtttt ttggtcatgg
     6961 gcgccgcggc atatcgtttc acggccgggg aggccgaacg ctccagatta cgccgggtat
     7021 taatgttggt aaccttcttc atctgttgtt tcgggattgg ggaatatttc ggaggagatc
     7081 ctatcgcccg gcaggatgtg acccgggtaa aaagtttctt tggcgatccc aactctctgg
     7141 gggccttttt aattttgact ttaccgctca tattcaaaga atggcaagaa accccgaaaa
     7201 gatcggtccg ttattttgct gcaggctgca ttctgttggg aagcgctacg ctgatcctta
     7261 ccttttccag ggcctcatgg ttagggttcc tggtgggact ggctctcttt atcctattcc
     7321 aagccgtggg tttaaccccc aaaaagctat tgaagatcat tgttatcgcc acagttattt
     7381 ggggtatctc atgggcgacc ggtagtgttt gggttggcgg ggccttattt tatctcctta
     7441 ttctcctggg tatggcttgg aatacagctg cacgtcgacc ctgggtcatc ggtctggtga
     7501 tcttggtcat cctgggggcg accatcggac aaaccttgac ctattttcaa ccaaggcaac
     7561 acagtgatta cgaattaaag gcccgggtaa cttccatagc ccaaggaaac gacagcgggc
     7621 gggggctgct ctggtctatt gggtggcgca tctttcaaga atctccctgg atcggccatg
     7681 gtttgggcag cttcagagat aacttccacc gctttgagac ccaggcagcg gtaaaatttt
     7741 ggggacccga ccgggatctc gatcaatctc ataatgaacc gctccattat ttagccactc
     7801 agggaatcct gggattcttg gcctatttcg ggttgttact ggcaatactg gcctctaccg
     7861 attggcgggg gttgttacgg cgcaatttgt cgctttcaga gaccagcctc ttctgcgcct
     7921 gcctgagtta tatgattttc gtaaattttg gttacgcact tgtccattat accttcatct
     7981 tctggatcga actcgggttg cttctgggtt accgtcatcc ggcaccccaa atccgtacct
     8041 ctccgacact tataccaacc tggctgttac cgctggcgct ctggatcttc atcattggag
     8101 gcggttggat tttgggcaat atgtttctgg ccgatctcaa ctaccgcacc gcatttcatc
     8161 aagcacggcg ccatcaattc gaggaatccc tggggagtta cagattggtt attgcccggg
     8221 cacccttaat tgagcattat cactatagat atgcgttgac gttatttcgg gcggccaagt
     8281 ggtatcaaaa acaggggggg gattcagaaa ccgcggatca gtattcccgg caatcccggg
     8341 ctattcttct gagattacgc cgaattcatc ccgatgagta tcagctcaac tttcttctgg
     8401 gccaggaata cgagaatggt tccgaatttc aggaggctgc agcttactat cgtcaggcgc
     8461 taatacaatt tccggagaat taccggctct atatccgcct cgccaaggct gaatggctgg
     8521 gaggtaacaa attggcttca cggcgggcct ttgatgccgg ttttgcgatg aaccccgaat
     8581 acgcacaaaa ggccgcgatc gcagaaggct taccgttgcc ctgatatcct acaccatcat
     8641 ctccagggta tgcagaacca tctttaccaa ctccggttcc ggcaacccgg tacgccagcg
     8701 aaagaccggg ggccttcccg gttggtaccg gaaatgattc cggtattttt ggagtacagc
     8761 caggacttta tcatagtcca tacccaagcc gatatggagt ttggcgataa gttcgtttcg
     8821 ttccttggtg agcgaggcca gcccgatccg tttggccaaa gccttaatct tggtgatatt
     8881 cagcagattc accaccggtt gcggcggctc gccgaaacgg tcgatgatct cttcggtaat
     8941 taggtcgaca tcctcgacgg tgcccgctgc ggcgatcttt ttgtaaacct cgactttctg
     9001 tttgttatcc gcaatatagt ccccgggaat ataggcatca gccggtacct ccactaccgg
     9061 ttcgggaagc tccggttgaa cttctccctt ccgttcccgg atcgcctcat ccaataaccg
     9121 gcagtatagt tcaaaaccaa ctgcagcgat ttgcccgtgc tgttccggac ctaacaagtt
     9181 cccggcgccg cggatctcaa gatcccgcat ggcgatttta aatcccgcac cgagttcggt
     9241 aaattcccta atcgcagcca ggcgtttctc ggcattctcc gacagcacct tatccttgcg
     9301 gtaacaaaag aatgcatggg ccaaacggtt actccggccg acccgacccc ggagttggta
     9361 aagttgcgcc agaccaaacc gttcggcatc ataaacaata agcgtattga cgttgggaat
     9421 atccaaaccg gtttcaatga tggtagtgca aaccaacaca tcggcttgcc cgtcatagaa
     9481 atccagcatc acccgttcca actgatcttc atccatctga ccgtgggcaa tggtaatcct
     9541 ggcgttgggc aatagattct gcaggtagga cgccatccgt tcaatcgtct caacccggtt
     9601 atatacaaaa taaacctgtc ccccacgatc caactcccgt tgaatcgcct cccggatcac
     9661 agcctcatta tattccagga catgggtccg gattgggtaa cggccttgag gggcggtttc
     9721 tataatactg atatcccgaa ttcccgccaa cgacatatgg agggtacgtg gaatcggggt
     9781 ggcggttaag gtcagcacat cgacattgtg gcggagctct tttaaccttt ccttatgggc
     9841 tactccgaac cgttgttcct catcgacgat taacaagccc aaatccttaa agtgcacatc
     9901 ggttgagagc aagcggtggg taccgataac caggtccacc tcgccgctga gtaaaccggc
     9961 aatggtctcc tcctgatcct gggcggtctg aaaacgacta agcgcgcgga ggttgatggg
    10021 aaacccggtt aatctctccc ggaaagtcaa ataatgctgc tgggccaaga tggtagtggg
    10081 gaccaacact ccgacttgtt tcccgtccat gatcgcctta aaggcggcgc gcatcgccac
    10141 ctcggtctta ccgtaaccga catcaccgca gatcaagcga tccatcggct tgtcgctctc
    10201 catatcccgc ttgatttctt caatcgcccg caactggtcg ggggtttcat caaagaagaa
    10261 cgattcctca aactcatgct gccatacggt atccgaggcg aacgtatgtc ccggggccac
    10321 ttccctccgg gcatacagat ccagtaattc ctcggccata tcgcgaatgg actcttttac
    10381 acggttcttg accttctgcc agtcggcccc gcctaactta ttcaacttgg gtggggcgtc
    10441 ttcgaccccg atgtatttct gaagcaggtt cacctggtcg gtggggacaa aaagcctgtc
    10501 ttccccggcg tactccaacc g
//
LOCUS       DASVMB010000485         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_499680,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000485 DASVMB010000000
VERSION     DASVMB010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499680"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1436
                     /locus_tag="VF531_13645"
     CDS             330..1436
                     /locus_tag="VF531_13645"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7715052.1"
                     /translation="MKSKIKVGLIYGGRSGEHEVSVLSANAVLSAIDREKYEVYPIGI
                     TKEGTWLPGQSPAPLVASEELQVRMLTDDSSQATANTALLKLENNKGRILAGLKDAVD
                     IIFPVMHGPFGEDGTIQGLLELADIPYVGGGVLASAAGMDKGVMKAIFRDAGLPVGDY
                     LVYTRHEWESGPERILKQIEAELGLPCFVKPANLGSSVGITKAHTQEELRKAMDLAAE
                     YDRRLVIEKMLAGKEIECGVLGNDEPMASIPGEVVPCADFYDYEAKYILNDSKLVIPA
                     PLNPEMTAKVQDLAVRAFRAVDCAGLGRVDFFVEPETKTVYVNEINTIPGFTRISMYP
                     KLWEASGVGYSELIDRLLQLALERHSDKKRNKLR"
     gene            1539..2285
                     /locus_tag="VF531_13650"
     CDS             1539..2285
                     /locus_tag="VF531_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7715053.1"
                     /translation="MWHVYADYHTHTCYSRGSGSIGDNARAAKAQGLEILGISDHAPA
                     NWGHLGTTDLKDFQRIMAETKKVQEEFSGLQILAGTEADIISYSGELDIPLTLQRQLD
                     QVLAGFHTTIIPKSLTEGFHFVSGRVLGLLSPSLKRKARTENTKAIVAAVYTNEIDII
                     THPGLHISIDTPELARACVKKETALEINARHGIKSVAFVQAAAKEGVKFAIGSDAHNP
                     KQVGRLEAGVKAAQLAGLTPDQVINVRDQK"
     gene            2416..3297
                     /gene="rapZ"
                     /locus_tag="VF531_13655"
     CDS             2416..3297
                     /gene="rapZ"
                     /locus_tag="VF531_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7715054.1"
                     /translation="MRFVIITGLSGAGKSETVKCFEDLGYFCVDNLPPILIPKFAELC
                     AQSDGRINRIALVVDIRGGSFFDDIFEALETLEQEGLSYEILFLEAREDILVRRFKES
                     RRRHPLSASGGITEGIRAEISRLEEIRGKATMILDTSDITVRGLRELINQHYMDQPEQ
                     ERMLITVVSFGFKNGIPLDADLVFDVRFLPNPHYVDSLRSLDGNFPEVANYVLKWPLA
                     LKFLSRLYDFMDFLAPQYVKEGKSNLIVAVGCTGGQHRSVTIANKLGDFLRTKGYRVV
                     VDHRDIPHLSQPETPTK"
     gene            3347..>4022
                     /locus_tag="VF531_13660"
     CDS             3347..>4022
                     /locus_tag="VF531_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX7715055.1"
                     /translation="MKSNQNGNVKGPKIVVIGGGTGLGTILRGLKLITSHLTAIVTVA
                     DDGGSSGRLRREYGMLPPGDIRNCLVALADLEPLMERLMQYRFTGDSELGGHSFGNLF
                     LAAMTEITGDFEVAIKESSNILAVRGRVLPATLADVTLNAELENGEIVRGESNVSKLK
                     LPIRRITLEPKNCMPVPEAIAAIKDADLIVLGPGSLYTSIIPNLLVCEVAQAIRNSGA
                     LKIYICN"
BASE COUNT         1019 a          871 c         1167 g          965 t
ORIGIN      
        1 gcgattacgg cggctacgcc gaaaagagca agatcaagat attccttccg agagccaact
       61 gatctacctt gccaaggagc ggttggtgaa gggtgagatt accccggaac agtatcgtga
      121 gattcgaaaa gaactgaaag aggcgtaagg tgtaaggcaa caggcaacgg gcaataggaa
      181 acagttaaag tccaaatcat attaggagat cgttcccctg ttgccggtta cccattccct
      241 gttgccaatt cttttgcaat ctgcaggaat ctccgtcccg atcaagaaat agcatctatt
      301 gacagatttg aaaaagcggg ggattcggta tgaagtccaa aattaaagtg ggtttgattt
      361 acggcggccg ctccggggag cacgaagttt ccgtactttc ggccaatgcg gtgctctcgg
      421 cgatagatcg cgaaaaatat gaggtttatc cgattgggat taccaaggaa gggacctggt
      481 taccggggca gtcgccggcg ccgctggtgg ccagcgagga gttgcaggtc cggatgctta
      541 cagatgattc atcccaggca acggcaaata ctgccctttt aaaactcgaa aataataagg
      601 gccggatttt ggccggcctc aaagatgcgg tggacatcat ctttccggtg atgcacggac
      661 ctttcggcga ggacgggacc attcaggggc ttttggaatt ggcggatatt ccctacgtcg
      721 gtggcggggt gctggcgtca gcggccggaa tggataaagg agtcatgaag gccatcttcc
      781 gggacgccgg tttgccggtc ggggattatt tggtttacac ccggcatgag tgggaaagcg
      841 gaccggaacg gatacttaag cagatcgagg ccgaactggg gttgccgtgt ttcgtcaagc
      901 cggccaacct cggttccagt gtcgggataa ccaaggccca cactcaggag gagttgcgca
      961 aggcgatgga tctggcggcc gagtatgacc ggcggctggt tatcgaaaag atgttggcgg
     1021 ggaaggagat cgaatgcggc gtattgggaa acgatgagcc gatggcctcg attccgggcg
     1081 aggtggttcc ctgcgccgat ttttacgatt acgaagccaa gtatatactg aacgactcga
     1141 agctggtgat tcccgctcct ctaaatccgg aaatgaccgc caaggtacag gatttggcag
     1201 ttcgggcgtt ccgtgcagta gactgcgcag gattgggcag ggtggacttt ttcgttgaac
     1261 ccgaaaccaa gaccgtatat gtcaacgaga ttaatacgat tcccggattt acccggatca
     1321 gcatgtaccc caagctttgg gaggcaagcg gagtcggtta ctcggagctg attgatcgcc
     1381 tccttcagct ggcgctggag aggcactccg acaaaaagcg gaataagctt cgttaagaaa
     1441 tttgctcata aaagaggagg cccaaaaggc cttcttttta ttcccctcaa aaactaatct
     1501 gcccggtcct gggcagaata gacccgaggt ggataaaaat gtggcatgtt tatgcggatt
     1561 accataccca tacctgctat tcccggggaa gcggcagtat tggggacaat gcccgggccg
     1621 ccaaggcaca ggggctggag attttgggta tctccgatca tgccccggcc aactggggtc
     1681 atctagggac tacggatctg aaagattttc aacggattat ggccgaaacc aagaaggtcc
     1741 aggaggaatt ttcaggccta cagattctgg cgggaactga agctgatatc ataagctatt
     1801 ccggagaatt agacatacct ctgacgttac agagacaatt ggatcaggtt ctggcgggat
     1861 ttcacacaac catcatcccg aaatcactga cggaagggtt tcattttgta agcggccggg
     1921 ttttggggtt attgagtcca agtcttaaac gaaaagcgcg gaccgagaat accaaagcca
     1981 tcgtggcggc tgtctatacg aatgagatcg atatcattac ccatccgggg ctgcatatca
     2041 gtatcgatac gcccgaactg gcaagagctt gtgtaaaaaa ggagacggcc ctggagatca
     2101 atgccaggca tgggattaag tcggtagcct ttgtacaggc tgctgccaag gagggggtaa
     2161 agttcgccat cggcagtgat gcccataatc ccaagcaagt agggcggctt gaggcgggag
     2221 ttaaggctgc gcaattggcg gggttaactc cggatcaagt gatcaatgtg agagatcaaa
     2281 agtaaatatt tgcaggtgga ttcggaggcg ggcagggttt aaaattagac tagaaataaa
     2341 ggcaggaaat gatcgggacg aagcgaatag ataaaaaact gatttttaga agaaggtgca
     2401 ggattgttgc aaaatattcg ctttgtgatc ataaccgggt tatccggggc cgggaaatcc
     2461 gaaactgtga aatgttttga ggatctcggc tatttttgtg ttgataattt accgccgatc
     2521 ttgatcccca agtttgcgga actttgtgcg caatcggatg gccggattaa tagaatagct
     2581 ttggtggtgg atatccgcgg cgggagcttc ttcgatgata tctttgaggc tttggagaca
     2641 ttggaacagg aaggtttaag ttacgaaata ctgtttctgg aggcccggga ggatatcttg
     2701 gtacggcgtt ttaaggaaag ccggcgacgt catcccctgt cggccagcgg cggtataact
     2761 gaaggaattc gtgccgaaat ttcaaggctg gaggagattc gcggcaaggc gacaatgatc
     2821 ctggatacct cggatataac cgttcgtggc ctgcgggaac ttatcaacca gcattatatg
     2881 gaccagcctg aacaggagag aatgctgatc acggtggttt cctttggctt taaaaacggg
     2941 atcccgcttg atgccgatct ggtttttgat gtccgttttt tgccgaatcc ccattatgtc
     3001 gattcgcttc gaagtttgga cgggaacttt cctgaagtgg ccaattatgt tttgaaatgg
     3061 cctttggcat tgaagttttt atcccggctt tacgatttca tggatttttt ggcaccccaa
     3121 tatgtgaaag aaggcaaatc caatctgatt gtagcggttg gttgcaccgg ggggcaacac
     3181 cgttcggtga ccatcgccaa taaattggga gatttcttac gaaccaaggg atatcgggtt
     3241 gtggtcgatc accgggatat tccgcacctg tcgcaacctg aaacgccaac caaataggtg
     3301 aatgaattac caactcaatc gggaagggtg agatgccacg tcttgggtga aaagcaacca
     3361 aaacggtaat gttaaaggtc caaagatagt tgtcatcggg ggtggaaccg gacttggcac
     3421 gatcttacgt ggcttaaaac taataacctc ccatctgacg gcgattgtta ctgtggccga
     3481 tgatggcggc agttctggtc ggttacgccg ggaatacggg atgttaccgc cgggggatat
     3541 tcgtaactgt ctggtggctt tggcagattt ggagccactc atggaaaggt tgatgcaata
     3601 taggttcacc ggagattcgg agcttggggg gcatagtttc gggaaccttt ttttggcggc
     3661 catgactgaa atcaccggtg atttcgaggt ggccatcaag gagtccagta atattctggc
     3721 tgtccgtggc cgggttctcc cggcgacatt agctgatgtc actttgaacg cggaattgga
     3781 gaatggcgaa atcgtccgtg gagaatccaa cgtttcgaaa ttaaagctac cgatccgccg
     3841 gattacgctt gaaccgaaga attgcatgcc tgttccggaa gcgatagctg ccatcaaaga
     3901 tgcggatctg attgttcttg gacccgggag tctctatacg agtattatcc caaatttact
     3961 cgtctgtgaa gtcgcccagg ccattcgtaa ctccggggcg ttaaagatct atatctgtaa
     4021 cg
//
LOCUS       DASVMB010000486         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_283122,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000486 DASVMB010000000
VERSION     DASVMB010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283122"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /gene="nrdD"
                     /locus_tag="VF531_13665"
     CDS             <1..1494
                     /gene="nrdD"
                     /locus_tag="VF531_13665"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEX7715056.1"
                     /translation="IPLGKLLESGFNNGHGYIRPPKRPSSATALAAIILQSSQNDMHG
                     GQSFAFFDRDMAPYVAGASEDETYQAMEALIYNLNSMHSRAGAQVPFSSLNLGCDTTP
                     DGRKVTKALLLAYEKGLGHGETPIFPNIIFRLKKGINFEPQDPNYDLFKFAMRVSTQR
                     LNPTFSFMDATFNEAYGSEVAYMGCRTRVMANRRGPAVTDGRGNLSFTTINLPRIAIK
                     AGGDLEIFFGELERVMNLAARQLYHRYRIQARLKVKDMPFLMGQGLYLDSDQLKPGDP
                     IAPVIKHGTLSIGFIGLAEALTALTSCHHGQDPQAQRLGLEIVNQMRKMVDGFAEEYD
                     LNYTLLATPAEGLSGRFVAMDRKRFGVIAGVTDKEYYTNSFHVPVNFDVSSFDKIAIE
                     GPYHELTNAGHISYVELQSPPLNNLEAYELLIRHMAACNMGYAGINFPIDECLGCYYR
                     GIIEEECPSCGSTHIRRIRRITGYLSTVDRFNDAKQAELRERHSHKI"
     gene            1529..2089
                     /gene="nrdG"
                     /locus_tag="VF531_13670"
     CDS             1529..2089
                     /gene="nrdG"
                     /locus_tag="VF531_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEX7715057.1"
                     /translation="MKSEKENFLIDQASQTTVRIAGVATESVVDGPGIRSTVFFQGCR
                     HACPGCHNPETWDTAGGRQVTLAELIDLLKLNPLISGVTFSGGEPFLQAGPAAVLGNH
                     LRKLGLNLWVYTGFTWEDLMNTPDHPENQALLEIATVVVDGPFRQQLKDLSLPFRGSA
                     NQRLIDAQASLAAGRVVIWSPDPGGW"
     gene            2089..2877
                     /gene="pgeF"
                     /locus_tag="VF531_13675"
     CDS             2089..2877
                     /gene="pgeF"
                     /locus_tag="VF531_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX7715058.1"
                     /translation="MMSRGWFRQEHQGFVFYRCPEFNNDRLIQAVSTRSCGNLALHTG
                     DQPGQVVARRTKVLEALGLHMERLVAAVQTHGTGIRVIDNAAAGAGALDYGTALPDTD
                     GLITATPGLILAIFTADCLPVFIYDPKTPAIGLVHAGWRGTLHGIVPKALDEMNRAFG
                     SIAAHCWVALGPGICSNCFQVSPDLAAKFAARRPQSVLCDQAGSKFRVDLVHYNQWLL
                     EQAGIRPERIIPSGFCTVCESGDFYSYRAEQGTNGRIISLLALA"
     gene            2949..4130
                     /locus_tag="VF531_13680"
     CDS             2949..4130
                     /locus_tag="VF531_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7715059.1"
                     /translation="MALQNETHRLSGGSPKHHRFKIYFSVLFRILLFIVIIEAGWLLV
                     RNLNLRPVVAKWGVIEKGYWTEALFLREETSIIAPISGIFETEITSGIRVPEGEFLAY
                     LHPETEQVTREEALQQYKLQARWQELTTEIKGQTMELNRIETALRRSKTASASRLGTV
                     REQQILNEESAHVRTYLAENQAEIKKLSVELSRNLLDQPVIFSAPVPGYCFFLHDGWE
                     GKLSPERILQLMPDDFKRNYRVRLTKTKVTAGDTVAKIINPFRQVVIIQPDTRRTGIP
                     QIGQSWWIKNAAGLNKVIINRVWTLPSGNAKSDLVVALDDSQLDRSLLPDRRLRVFLV
                     YRRVTGIVIPKRAVIKNGQTIVRVMKGDGYTKQPVKILESDDSNVIVSGLDFGTTIIS
                     R"
     gene            4142..>5342
                     /locus_tag="VF531_13685"
     CDS             4142..>5342
                     /locus_tag="VF531_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7715060.1"
                     /translation="MKKVFVLDTSVLVHDPQALNAFQDNDIIIPLAVVEEIDGHKKRQ
                     DEVGRNARWVSAFLDKLREKGHLDHGVSLGENRGIIRVELNHQEVNNLPPALDRTKID
                     NRIIAVASALKNSSALPVVLVTKDINMRIKADALGIAAEDYETDKIDIEEVYTGIRTV
                     QVEPAQIDRFYSAGSLDLPEMELFPNQMIILEDAFGSSKSALARHLNGGRLVPLRYGN
                     TDPFGLKPRNKEQRFALELLLDEDIRLVTLIGKAGTGKTLLALAAGLQKVVEESKYRR
                     LAVSRPVIPMGNDLGYLPGDIDEKLRPWMQPIYDNLEFLFSNRDQNEKIDQLVNSMKD
                     LNILELEALTYIRGRSIPNQFLLIDEAQNLSPHEIRTAITRVGEGTKIVLTGDPYQID
                     HPYLDSNS"
BASE COUNT         1330 a         1269 c         1412 g         1331 t
ORIGIN      
        1 attccattgg gaaaacttct ggagagtggt ttcaataacg gccatggtta cattcgtcct
       61 cccaaacgtc cctcatcggc cacggctttg gctgctatta tcttgcaaag ttcacagaat
      121 gatatgcacg gcggccaatc atttgcattt tttgatcggg atatggcccc gtatgtggca
      181 ggtgcctcgg aagacgaaac ctatcaagcc atggaagcct tgatttataa tctcaattcc
      241 atgcatagcc gggcaggggc gcaagttccc ttctccagcc tcaacctggg atgtgatacc
      301 actccggacg gccgcaaggt aaccaaggcg ttgctgcttg cgtatgaaaa aggtttaggc
      361 catggtgaaa cgccaatctt tcccaacatt attttccggt tgaaaaaggg tattaacttt
      421 gagccgcaag accccaatta cgatctcttt aagttcgcga tgcgtgtctc gacccaacgg
      481 ctcaatccca ccttttcctt tatggatgcc acatttaacg aagcctacgg ttccgaggta
      541 gcctatatgg gttgccggac ccgggtaatg gctaatcgtc gcggccccgc cgttaccgat
      601 ggccggggta atctttcgtt tacaaccatt aacctgcccc ggatcgccat caaggcagga
      661 ggggatctgg agatcttctt tggcgagttg gaacgggtga tgaatttggc ggcccggcag
      721 ttgtatcatc gttaccgaat tcaagcccgt ctcaaagtga aggacatgcc ttttttaatg
      781 ggtcaagggt tatatctgga ttcggatcaa ttgaaacccg gagatcccat cgcacctgta
      841 atcaaacacg gtaccctgtc catcggcttc attggcctgg cagaggcctt aaccgctctg
      901 accagttgcc atcacggcca ggatcctcaa gctcagcgat tgggactgga gatcgtcaat
      961 cagatgcgga agatggtgga tggtttcgcg gaggagtacg atctgaacta caccttactc
     1021 gccactccgg cggaaggact atccggccgc tttgtagcaa tggatcgaaa acgtttcggg
     1081 gttattgccg gagtcacaga caaggaatac tataccaata gttttcatgt tccggtgaac
     1141 tttgatgtct catcctttga caagatcgcc attgaaggac cgtatcatga attgaccaat
     1201 gcgggacaca ttagctatgt ggaactacaa tcgccgccgc taaataatct ggaggcttat
     1261 gaactgctga ttcgtcatat ggccgcctgt aacatgggtt acgccggaat aaactttccc
     1321 attgacgaat gtctcggttg ttactaccgg ggcatcattg aggaggaatg cccatcgtgc
     1381 ggttctaccc atatccggag aatccgtcgg atcaccggtt atctctcgac tgtggacagg
     1441 tttaatgatg ccaagcaagc ggaactgcgt gaacggcata gccataagat ataacccgcg
     1501 gaaattaata gacaagttag gtaagtaaat gaaatcggaa aaggaaaatt ttttaataga
     1561 tcaagcatcc caaacgacgg ttcgcatcgc gggagttgct acagaaagcg tagtcgatgg
     1621 cccggggatc cgtagcacgg ttttttttca aggttgccgg catgcctgcc cgggttgtca
     1681 taatcctgaa acctgggaca cagcaggagg ccgtcaggtt acattggcgg aactcatcga
     1741 cttacttaaa ctaaatccgc tgatctccgg tgtgactttc agcggtggag aacctttttt
     1801 acaagccggg ccggcggcgg ttttaggtaa tcaccttaga aaactggggc ttaatctatg
     1861 ggtttacacc ggttttacct gggaagatct gatgaatact ccggatcacc ccgaaaacca
     1921 ggcgctctta gagattgcca ccgtggtggt agacggccca ttccggcagc aactgaaaga
     1981 tttaagctta ccttttcggg gttcggctaa tcagaggttg attgatgccc aggcgagtct
     2041 ggctgcgggt agggttgtga tatggtcgcc ggatcccgga ggttggtaat gatgtctagg
     2101 ggctggttcc gccaggaaca tcaggggttt gtattctatc gttgcccgga gttcaataac
     2161 gaccgcctga ttcaggcggt ttcgacccgg agttgcggta atcttgcttt gcataccggt
     2221 gatcaacccg gacaggtagt ggcaaggcgt accaaagtac tggaggcact gggacttcat
     2281 atggagcggt tggtcgctgc ggttcagacc catggtaccg ggatcagggt gatcgataac
     2341 gccgcggctg gtgccggtgc gctggactat ggcaccgccc tgccggatac cgatggtttg
     2401 atcaccgcaa ctcccgggct gatactggcg atcttcaccg ctgattgctt acctgtattt
     2461 atctacgatc caaagacccc ggccatcggg ctagtccatg ccggatggcg cggcaccctc
     2521 catgggattg tcccgaaggc cttagatgaa atgaaccggg ctttcgggag tattgccgct
     2581 cattgttggg tagcgcttgg cccgggaatt tgttccaatt gttttcaggt aagtccggat
     2641 ctggccgcga aatttgcggc ccggcgaccg caatcggtat tatgcgacca agcgggatca
     2701 aagtttcgtg tcgatttggt gcattataac caatggctgc tcgaacaggc cggaattcgt
     2761 ccggaacgaa tcattccttc cgggttctgc actgtctgtg aatccggaga tttttattca
     2821 tatcgagccg agcaaggaac aaacggccgg attatctccc tcctggctct ggcgtaaacc
     2881 ggccatgacg aaatagtcca atacatcaag gaatatgatg gaaaacgtga taacggagcg
     2941 attcgatcat ggcccttcaa aatgaaactc atcggctttc aggtggttca cccaaacatc
     3001 accggtttaa gatttatttc agtgttttat tccgtatctt gctttttatc gtcattattg
     3061 aagctggatg gcttttagtc cgtaatctga atctccggcc ggttgttgcc aaatggggtg
     3121 taattgagaa aggatattgg actgaagcct tgtttttacg ggaagaaacg agcattatcg
     3181 ctccgatatc cggcatcttt gagacggaaa taacttcagg catccgagta ccggaaggtg
     3241 aatttcttgc ttacctccat cccgaaactg agcaagtcac ccgggaagag gcgttgcaac
     3301 aatataagct gcaagcgaga tggcaggagt taaccacgga gattaaaggc caaactatgg
     3361 agttaaaccg gatcgaaacc gctctccggc gaagtaaaac agcatcggca agccggttgg
     3421 gcacggttcg ggagcaacaa atattaaatg aggaatcagc ccatgttcgc acctatctgg
     3481 cggagaatca ggcagaaata aagaagctgt ctgttgaatt atccaggaat cttctggacc
     3541 aaccggtaat attttccgca ccggttcccg gttattgttt ttttcttcat gatggctggg
     3601 agggaaaatt gagcccggag cgaattctac agttaatgcc cgatgacttc aagcggaatt
     3661 atcgggtgcg gctgaccaaa acgaaggtaa ctgcgggtga tacggtcgcc aaaatcatta
     3721 acccctttag gcaagtcgtt atcattcaac ccgatacccg acggactgga attccccaaa
     3781 tcggtcaaag ctggtggatt aaaaacgcgg cggggttgaa taaagtaatt attaaccggg
     3841 tctggacttt accgtccgga aacgccaaat cggacctggt ggttgctctc gatgatagtc
     3901 aattggaccg gagcttactg ccggatcgcc gcttgcgggt attcctggtt taccgccggg
     3961 tgaccgggat cgtaataccg aaacgggctg ttatcaaaaa cggccaaaca atcgtccgcg
     4021 tcatgaaagg agatggttat accaaacaac ccgtaaaaat cctggaatcc gacgattcca
     4081 acgtgatcgt ttcaggtctt gatttcggta cgaccatcat ttcacgatag ggggtctact
     4141 gttgaagaag gtttttgtct tggataccag tgttttagtt catgatcccc aagctttaaa
     4201 cgcttttcag gataatgata tcatcatccc gctcgcggtg gttgaagaga ttgacggtca
     4261 taaaaaacgc caggatgaag tgggccggaa tgcccgttgg gtctcggctt ttttagataa
     4321 attacgtgaa aagggacatt tggaccacgg agtgagtctc ggggagaacc ggggtatcat
     4381 tcgagtggaa cttaatcatc aggaggtcaa caacctcccg ccggctctcg accggaccaa
     4441 gatcgacaac cggattattg ccgtcgcttc ggctttaaaa aatagttcag cattgcctgt
     4501 ggttctggtc accaaggata tcaatatgcg aatcaaggct gatgcattgg gaatcgcggc
     4561 ggaggactac gagaccgaca agatcgatat cgaagaagta tataccggga ttcgtactgt
     4621 ccaggtagaa ccggcccaga ttgatcgttt ttatagcgcc ggcagtcttg accttccgga
     4681 gatggagctt tttcctaacc aaatgatcat tttggaggat gccttcggtt cttccaaatc
     4741 ggcactggcg cggcatttaa atggtggccg gctggtgccg cttcgttacg gcaacacgga
     4801 tccttttgga ttaaaacctc ggaacaaaga gcaacggttt gcgttggaac ttttattgga
     4861 tgaggatatc cggttggtaa cgctgatagg caaggccggg accggtaaga ctttgctcgc
     4921 cctggcagcc ggactccaga aagtagttga ggaatcaaag taccggcgtt tagctgtttc
     4981 aaggccggta atccctatgg gcaacgatct cgggtatctt cccggggata tcgacgaaaa
     5041 actacgcccg tggatgcagc cgatctacga taatctagag tttctattca gtaaccggga
     5101 ccaaaacgaa aagatcgatc aattggtgaa cagtatgaag gatttgaaca ttctggagtt
     5161 ggaggcgctc acctatatca ggggacggag tattcccaac cagtttctcc tgattgatga
     5221 ggctcagaac ctctcacccc acgaaattcg taccgccatt acacgggtcg gtgaagggac
     5281 caagatcgtc ctgaccggag atccatatca gatcgaccat ccctatcttg attccaatag
     5341 ta
//
LOCUS       DASVMB010000487         7639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_429502,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000487 DASVMB010000000
VERSION     DASVMB010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429502"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF531_13690"
     CDS             <1..369
                     /locus_tag="VF531_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7715061.1"
                     /translation="SIGVPIAVATNYSFIAETATMTIHPLRLSGLVVGVPQTYEYLDK
                     MQDRVIKFVTNHSKVSEERFRELMFKTGDLVRDIGTVLVGKDAVSCGLINEVGGISQA
                     LKKLDEIVELKYGAGRKGLQ"
     gene            366..596
                     /locus_tag="VF531_13695"
     CDS             366..596
                     /locus_tag="VF531_13695"
                     /inference="COORDINATES: protein motif:HMM:NF025401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HEX7715062.1"
                     /translation="MMLYTIVPMEAIFVEEESTGIGVSSLPSPIMIRRDGIDLLVEQQ
                     PGGEYRIQRLISTNPRDFLNPKWQPGSILSIS"
     gene            901..2610
                     /locus_tag="VF531_13700"
     CDS             901..2610
                     /locus_tag="VF531_13700"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX7715063.1"
                     /translation="MTSVVEGLLSLAGAMGIFLYAISRFGDGVQKMAGERIRAVLETQ
                     ATEPLSSVGTGIGIAAALQSNVLTFGMISSLVNAGLLGLLPAVLTMLGVNLGITVTAQ
                     LISINIGAFAFLLLFIGYAMNFYGKKRNWHYLGQVIFNLALMYIGFELFHRAFQVLAF
                     DPTAARLIKSAFANPWLGFLIGLSLATLLRSSNTVVVLVQGIVGVTALFSPSLIGGAF
                     AIIIGANVGTTIINMLVGLDRLPIAKKANWLHFTFNFLTALFWLALLPIAISGVHAVS
                     SELGSAFQWFEVAVFRWQVPAFAAGRWFNIWELAMAHTLFNTSVILIWLPVSLMASKL
                     GFSLFAEKGKIGVSGTTYLDRRALQSPALALILACHEINQMAMITLEMLKSARLSFLK
                     GQVHLIAGIAGDETIVDDLQEQITFYLSALLSQNSLTESQSHRLAGLLHIVSDIERVG
                     DHANNIANLAEKKHQEQLPFSEIALNEMELFFGKTMDVYSKACQSLRENNLELAKQIQ
                     TREANIDKLEEELQQNHIHRLNQGKCWPGSGVVYVEILSNLERVAAHSANISVVILEE
                     GEE"
     gene            2610..4901
                     /locus_tag="VF531_13705"
     CDS             2610..4901
                     /locus_tag="VF531_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX7715064.1"
                     /translation="MAGSKDKPIEEPQEEAEPKDDRLVEQRFEITGVLLIGLALLSFV
                     SILFQSAGSIGEILKRGLFYAFGRRGAIIPVFFLAIIGWSLLRWRRGVRLTRHFIALF
                     LLFVWALLIMHICSGVKITSNVGASVIEGGGVIGNFMAWHLLAWFGNWGTGIFIFIWL
                     VIAAILALDRPLIEVIFGLGGRVKIHRKLRELEPEPAETAATASGTPKFRPDEMEIDH
                     AAATKNPNNLISFPAKERRQSQVLNTLQHMLKEKEQQKVEPEDVPEIRKTPEIRIETR
                     NAAPGQTSPVKINTVSGPYRLPEANLIGPSGLKAKKDLRILDQSRQLQETLNSFGVQA
                     KVIETHFGPVITRYDLQPAPGVKVSKIVNLADDLSLALAAKGLRLEAPIPGKAAIGVE
                     VPNQEAQVVTFRDILDSKTFWNSGKLSFALGVDIGGEVVVTDLRKMPHLLIAGATGSG
                     KSVCVNTLIMSILFKAFPNEVKFIMIDPKMVELSMYNGIPHLMAPVVTEAKKAAGVLK
                     LVVDEMERRYKMFAEARVRDLDKYNQQVPETEALPVIVVIIDELSDLMMVAPVEVEDA
                     ICRLAQMARATGIHLVVATQRPSVDVITGLIKANIPSRIAFAVSSQIDSRTILDSVGA
                     ERLLGRGDMLFAPVGAMKPRRIQGALVTEKEIEAVIKHWREQGEPKYQEQFVNVPEKK
                     DEIDAEADDLFWDAVRIVVDYGQASASILQRRLRIGYARAARLVDMMESKGMIGAYEG
                     SKPREVFITARQMEEIRKRSQDQ"
     gene            5094..5861
                     /locus_tag="VF531_13710"
     CDS             5094..5861
                     /locus_tag="VF531_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX7715065.1"
                     /translation="MKEIGEYLHRVREEKNISLKEVQESTKISLRYLEAIDKGDFESI
                     PGEVYRKGFLVNYATALGLDGQDILKKYYELKAQEEEQQRKVQLEQAIQNKNTPVINL
                     NWSKEVYLGIVAVLIASLIIISFFAFPSFNNTPYVTEEEAVSGEPAEQPVEDSQGRIN
                     KQLLPAPIAVYASFKEAVWVSVKADGVYLYTSDGTTIDPSSPKLLWTAQRELEIRLGN
                     PAGVNLSFNGKDMGKLGERGVVKLFKFTPKGLVTQTP"
     gene            5911..>7639
                     /locus_tag="VF531_13715"
     CDS             5911..>7639
                     /locus_tag="VF531_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7715066.1"
                     /translation="MPDFLTKPGKYGKQFLARAGEIMELDRNRELYKEIKEKNDIVAV
                     ISEYVTLKRSGRSLVGLCPFHSEKTPSFNVNPAKQFYYCFGCSSGGDVFSFIMKMENL
                     EFIDAAHLLADRAGILWPEERQITEVDRRREYFYRINQLAAAFFNQCLEKTTIGERGR
                     LYLEERGLTSETRHRFLLGYAPRGWHSLTEILRKKQVPLEQAAELGLVGFGASGYYDR
                     FRDRVIFPISDPKGRIIGFGGRIIATNVPPSGFEEPVHTLMQDSGSASLNTDPGQETV
                     EAERTAAERPLPKYLNSPDSPLFRKGSFLYGLSLAKDYIRKKNQAIIMEGYLDVIQAH
                     QAGIREAVASLGTALTHEQARLIKRYTDEVVLAYDADAAGQHATVRGMELLEEAGLRV
                     RILALPPGDDPDSFIKSEGADAFNHLVASAKNLTDFKISLALREYNLTTPEGKSQAVQ
                     SILPVLGGIENNITREEYIRQLAREIGVSETAIFEELRKWLQGHRKKSPDLDRNDNRS
                     YTKESNEQNGVFWGNIEGAEASPLKQAIFEAEKELLRSALQEYDKFERIKVELIAEEF
                     TFKVWRDLFT"
BASE COUNT         1895 a         1722 c         2071 g         1951 t
ORIGIN      
        1 tctatcgggg taccaatagc tgtcgcaacc aattacagtt ttattgcgga aacggctacc
       61 atgaccattc atccgttacg gttaagcggg cttgtggtgg gggttcccca aacatacgag
      121 tacctcgaca agatgcagga ccgggtgatt aaattcgtaa ccaatcactc caaggtctcg
      181 gaggaacgtt ttcgagaatt gatgttcaag accggtgatc tggtccggga tatcggtacc
      241 gtgctggtag ggaaggatgc ggtatcctgt ggtttaatca atgaagtcgg cggtattagc
      301 caagccttga aaaagcttga tgaaatcgtc gaactcaagt acggcgcggg gcggaagggg
      361 ttgcaatgat gttgtacact atcgtaccca tggaggcgat ctttgtcgag gaggaatcca
      421 ccgggatcgg tgtgtccagt ctcccctccc ccatcatgat ccggcgggat gggatcgatc
      481 tcttggtgga acaacagccg ggcggggaat atcggatcca gcggttgatc agtacgaacc
      541 cgcgcgactt tttaaatccc aaatggcaac caggctcaat cctgtcaata tcatgaaccc
      601 ccgaaaaggg ggtttttatt ttccttgagt tacgttatac tgaaaaagga tataaccggc
      661 aagtaaaaaa cctctggatt tggacgttta taaccggcag tcgaaagcaa tcaatgtcaa
      721 agactgcagg aattgcctga aggttcgaga atataaatgg aggtttcacc atcatctctc
      781 tccttccgcg gagaataggg gaagcctttg tatttcaaga tttcttggaa attttccgga
      841 aaatataagt actttcgtga aaatcgtaac tttgcatatt tcattaggag gcaaaataga
      901 gtgacgtcag tcgtcgaagg cttattgagc ttagcgggtg cgatgggtat ttttttgtat
      961 gccatctccc ggtttggtga cggggtccaa aagatggccg gagagaggat cagagcagtt
     1021 ttggagaccc aggccaccga gcccctttcc agcgtaggca cgggaattgg aattgccgcg
     1081 gctctacaga gcaatgtgct gacttttgga atgatttcaa gcttggtgaa tgcgggactg
     1141 ttgggcttac ttcccgcagt cctgacgatg ctcggcgtga atctgggaat taccgtaacc
     1201 gctcagttga tctcgattaa tatcggcgct ttcgctttct tgctcttatt tatcggatat
     1261 gccatgaatt tttatggcaa gaaacgaaac tggcactatc tcggacaggt tattttcaat
     1321 ctggccctga tgtatatcgg ttttgaactg tttcatcggg catttcaagt gttagccttc
     1381 gatcccacgg cggcccgttt gattaagtcg gctttcgcca atccctggtt gggttttctg
     1441 atcggtttga gtctggcgac actgctgcgc agtagcaaca cagtggttgt cttggtccaa
     1501 gggattgtgg gtgtaacggc attattctct ccgtcactta tcggtggcgc tttcgccatc
     1561 atcatcggcg ccaacgttgg tacgaccatc atcaatatgt tggtaggcct ggaccgtttg
     1621 ccgattgcga aaaaagccaa ctggctccat ttcacgttta actttttaac cgcccttttt
     1681 tggctggctc tattacccat cgcgatctca ggggttcacg cagttagctc cgaattaggc
     1741 tcggcatttc aatggttcga ggtagcggtc ttccgctggc aggttccggc ttttgccgca
     1801 ggccgttggt ttaatatttg ggagttggcg atggctcaca ctctctttaa taccagtgtg
     1861 atcttgatct ggcttcccgt cagccttatg gcatccaaac tcggtttctc gctttttgcc
     1921 gagaaaggga agattggggt tagcggcaca acctatttgg atcgccgggc tttgcaaagc
     1981 ccggcattgg ctcttatcct ggcttgccat gaaatcaatc agatggcaat gatcaccctg
     2041 gaaatgttga aatcggcacg actttccttt ttaaaaggcc aggttcatct catcgccggt
     2101 attgccgggg atgagactat cgtcgacgac ttgcaggaac agattacatt ttacctctcg
     2161 gcgttgttat ctcaaaattc actgaccgaa tcgcaatccc atcggctggc gggactgctg
     2221 catattgtca gtgatattga acgggtcggg gatcatgcca acaatatcgc caatctggct
     2281 gagaagaaac atcaggagca actgcctttc tcggagattg cactgaacga gatggaattg
     2341 ttcttcggta aaacgatgga tgtttattcc aaggcatgtc aatctctccg tgaaaacaat
     2401 ctggaactcg ccaagcagat acaaacccgc gaagctaaca ttgataaatt ggaagaggag
     2461 ttgcaacaaa accatattca ccggttaaac cagggtaaat gctggccggg atcgggtgtt
     2521 gtctatgtcg agatcctcag taatcttgaa cgggtcgcgg cgcattccgc caacatttcc
     2581 gtggtaatac tggaagaggg tgaggagtaa tggccgggtc gaaagataaa ccaatagaag
     2641 aaccgcagga agaagccgaa cccaaggatg atcgccttgt tgagcagcgc tttgagatta
     2701 ccggggtatt attaatcggt ctggctttac tgtcttttgt ttcaatctta tttcagagcg
     2761 ccggttctat cggcgaaatc ttgaagcgag gcctgtttta tgccttcggt cgccgcggcg
     2821 ccatcatccc cgtatttttc ctggcaatca tcggttggtc attattacgc tggcggcgcg
     2881 gggtacgcct gacccgccat tttatcgcgc tgtttctttt atttgtctgg gccttgttaa
     2941 ttatgcatat ctgtagcggg gttaagatca cttccaacgt cggcgcatcg gttatcgaag
     3001 gtgggggtgt aatcggaaat tttatggcct ggcacctgtt agcctggttc ggtaattggg
     3061 gtaccgggat ctttatcttt atctggctgg tcattgccgc aattttagcc ttggaccggc
     3121 cgctgatcga ggtgatcttt ggtctgggag gccgggtgaa gatccatcga aaacttcgag
     3181 agctggaacc ggaaccggcc gagacggccg ccacggcaag cgggactcct aagtttcgcc
     3241 cggatgaaat ggagatcgat cacgccgccg cgacaaaaaa tccgaataat ctgatttcat
     3301 ttccggccaa ggaacgccgt cagagccagg tgttgaacac attgcagcat atgttgaaag
     3361 agaaggaaca gcaaaaggtc gaaccggaag atgttccgga gattcggaag accccggaaa
     3421 ttcggatcga aacccgtaac gctgctccgg gccaaacctc tccggtcaag attaatactg
     3481 tttccggccc ctaccggctg cctgaagcca atttgattgg accctccgga ctcaaggcca
     3541 aaaaagattt gcggatcctg gatcaatccc gccagctcca agagacatta aacagtttcg
     3601 gtgtccaggc taaagtgatc gagacgcact ttggtccggt gatcacccgt tacgacctgc
     3661 aaccggcccc gggagtcaag gttagcaaaa ttgtaaatct ggctgacgat ctctccttgg
     3721 cgcttgcggc caaaggactt aggcttgaag cgccgattcc gggcaaggca gccattggcg
     3781 tcgaggtccc caatcaggag gcccaagtag ttaccttccg ggatattctc gactccaaga
     3841 ccttttggaa ctcaggaaag ctcagcttcg cgctgggggt ggatatcggc ggggaagtgg
     3901 ttgtcaccga tttgcgcaag atgccgcact tattgatcgc cggtgccact ggttccggga
     3961 aaagcgtttg tgtcaatact ttgatcatga gcatcctttt taaggctttt cccaatgaag
     4021 tcaagtttat catgatcgat cccaagatgg ttgaactctc gatgtataac gggattcctc
     4081 atttgatggc cccagtggtc accgaggcca aaaaggcggc cggagttctt aaactggtag
     4141 tcgatgagat ggaacggcgc tataagatgt ttgccgaagc cagggtgagg gatctggata
     4201 aatataacca gcaagtgccc gaaaccgagg cgctcccggt gatcgtggtc atcattgacg
     4261 agctctccga tctgatgatg gtcgctccgg tagaggtgga ggatgccatc tgccgtttgg
     4321 cacaaatggc ccgggctacg ggaatccatt tggtggtcgc tacccaacgc ccctcggtcg
     4381 atgtaatcac cgggcttatc aaggccaata ttccttcacg gattgcgttt gctgtttctt
     4441 cgcagatcga ctccagaacc atcctggaca gtgtaggggc agaacgctta ctggggcggg
     4501 gcgatatgct tttcgcaccg gtaggggcga tgaaaccccg caggattcaa ggggctttag
     4561 tcacggagaa ggagatcgag gcggtgatca agcactggcg tgagcaaggc gaacctaagt
     4621 atcaggaaca gtttgtcaat gtgccggaga agaaagatga gattgatgcg gaagcggatg
     4681 accttttttg ggatgcagtc cgaattgtgg ttgactacgg tcaagcttct gcctccatac
     4741 ttcagcggcg gttaaggatt ggctatgccc gtgcggcaag actggtggat atgatggagt
     4801 ccaagggcat gatcggtgct tacgagggca gtaagccgcg ggaggtattt attaccgccc
     4861 gtcagatgga ggagattagg aaacgaagcc aagatcagta attttgatct ttgaggaagg
     4921 aactgtcctg attaactcga atatatacat atgcttatga gccatttcct gcggatgtgt
     4981 tccgtgccac aagattcagc ttcaagagct ttgggacagg ttgttccggt tgatccgtgg
     5041 acatctttaa cttgaaatca taagctataa cccatttcaa gggggtctgg ggtttgaaag
     5101 agattggaga atacctccat cgggttcgtg aagagaagaa tatctcccta aaagaggttc
     5161 aggaatcgac caagatcagc ctgaggtatc tggaagcaat tgataaaggt gattttgaaa
     5221 gcattccggg tgaagtatac cgcaagggtt ttttagtcaa ttacgctact gcactcggcc
     5281 ttgacgggca ggatatttta aagaaatact acgaactcaa ggcacaggag gaggaacaac
     5341 agcgtaaggt ccaacttgaa caggcgattc agaacaaaaa caccccggtt atcaatctga
     5401 actggtccaa agaagtatac ctggggatcg tggcggtgct gatcgcttct ttaattatca
     5461 tcagtttctt tgctttccct tctttcaata atacccctta tgttacggaa gaagaagctg
     5521 tctccgggga accggcggaa caaccggttg aggattcaca aggccggatc aataaacagc
     5581 ttttaccggc cccaatagcg gtttatgcca gtttcaagga agcagtttgg gtcagtgtca
     5641 aagccgacgg cgtatatttg tataccagcg atggtacaac cattgatcct tcatccccca
     5701 agttactctg gactgcgcaa cgggagttgg agatccggct gggcaatccc gcgggtgtaa
     5761 atttgagctt taacggaaaa gatatgggaa agctcgggga acggggtgtg gtgaagctgt
     5821 ttaagttcac gccgaaagga ttggtaacac aaacgcccta agggcgtttt tttttcctat
     5881 gctttatctt tttatgaaga gtcattttgc atgccggatt ttttaacgaa accgggaaaa
     5941 tatgggaaac agtttttggc aagagcgggt gagatcatgg agctggatcg gaatcgggaa
     6001 ctatataagg aaattaagga aaaaaacgat attgtcgctg tgatctccga atacgtgact
     6061 ttaaaacgtt ccggacggtc cttggttggt ctctgcccgt tccactccga aaagacgcct
     6121 tcttttaacg taaatccggc caaacagttc tattactgtt ttggttgcag cagcggcgga
     6181 gatgtcttca gtttcatcat gaagatggaa aatcttgaat tcatcgatgc ggcccatctt
     6241 ttggcggatc gggccggtat tttatggccg gaggagcgcc agatcacaga ggttgatcgc
     6301 cgcagggaat atttttatag gattaaccaa ttggcagcgg cttttttcaa ccagtgcctc
     6361 gaaaaaacaa ccattggtga acggggacgg ctctatctcg aggaacgcgg cctaacctcc
     6421 gaaactcggc ataggtttct tctgggatac gcgccccggg gatggcatag cttaaccgag
     6481 attttgcgaa aaaaacaagt tccgctggaa caagctgccg agctgggctt ggttggcttc
     6541 ggcgcaagcg ggtattatga cagatttcgg gatcgggtca tttttccaat cagtgatccc
     6601 aagggacgta tcattggttt tggaggccgc attatcgcaa ccaatgttcc cccctcgggt
     6661 ttcgaagaac cggtacatac cctgatgcag gattccggaa gtgcttcttt gaatacagac
     6721 ccggggcagg aaactgtcga ggcggaacgg accgcggcgg aaaggccgct tcccaaatat
     6781 ctgaactcgc cggattcgcc gctgtttcgt aaggggtcct ttttatacgg cttgtcgctt
     6841 gccaaggact atattcggaa aaagaatcag gctattatca tggaaggtta tctcgacgta
     6901 atccaagccc accaggcggg aatccgggaa gccgttgcct cgctgggaac ggcgctcacc
     6961 catgaacaag cccgtttgat caagcgctac actgacgaag tggttttggc ttatgacgcc
     7021 gacgccgccg gtcagcacgc aacggtccgc ggaatggaac tattggaaga agcggggctt
     7081 agggtacgga tccttgccct gcctcccggg gacgatccgg actcctttat caagtccgag
     7141 ggagcagatg cttttaatca tttggtggcg tcagccaaaa atttaaccga tttcaaaatt
     7201 agtttggcgt tacgagaata taatttaact accccggaag gtaaatctca ggcagtacag
     7261 agtatcctgc ctgttctggg ggggatcgag aataatatca cccgtgagga atatatccgg
     7321 caactcgcca gggaaattgg cgtttcggag acagccatct ttgaagagct gcgaaaatgg
     7381 ttgcaaggtc acaggaaaaa atctccggat ctggatagga acgataatag gagttatact
     7441 aaagaatcga atgaacagaa tggggttttt tggggaaata tagagggtgc ggaggcctca
     7501 ccattaaagc aagccatttt tgaagcagag aaggaacttt tacgatctgc tttgcaggaa
     7561 tatgataaat ttgagcgaat taaagtagaa ttaatagcgg aggagtttac tttcaaggtt
     7621 tggcgggact tattcaccg
//
LOCUS       DASVMB010000488         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_429645,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000488 DASVMB010000000
VERSION     DASVMB010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429645"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF531_13720"
     CDS             complement(<1..245)
                     /locus_tag="VF531_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcobamide--CoM methyltransferase"
                     /protein_id="HEX7715067.1"
                     /translation="MIREPLSKQEVIQAIERKGGTRIPLVFHKWWGKGLEAKYGTALE
                     TIGAAIPEDIMAVFYRDPGEDRSFNNNPSYRWGYRDD"
     gene            complement(282..1607)
                     /locus_tag="VF531_13725"
     CDS             complement(282..1607)
                     /locus_tag="VF531_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010348384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7715068.1"
                     /translation="MKQIQWVYTNQREQWVPGRKFHRSEQGEANLAIVDEPHPEWMGF
                     GGCFNELGWQALQALDSDARQELLRDLFEPGYGCQFNFCRVPVGASDYASSWYSLNET
                     EGDLGMEHFSIERDRQSLIPYIQSAQNLCPSLRLFASPWSPPTWMKYPRAYNFGTLIW
                     ERPVLAAYALYLLKFVQSYAALGIPIQQLHVQNEPVSDQKIPSCIWTGAQLRDFIRDY
                     LGPLFEKHRVTTEVWLGTINGPEVDERYLRTTYNDYANLVLSDPHARKYIGGVGYQWR
                     GKYAVQRTRQGWPEIPVIQTENECGDGNNSWEYAAYVFDLFYHYLTNDVIAYVYWNMV
                     LETGGESTWGWQQNSLVSVDPKTGRITRNPEYYIMKHFSKFIHPGDHRLGLVKPWSAN
                     AVAFRSAAGRTVVIRNPFGDQALLCLAIDDAVYNFELQPHSVNSILIAD"
     gene            complement(1632..>1916)
                     /locus_tag="VF531_13730"
     CDS             complement(1632..>1916)
                     /locus_tag="VF531_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715069.1"
                     /translation="MFKIVAPVAKPAIITTAIMTFINNWNEFIMVNTFIRTDNLRTLP
                     FSVYKFTGEYVSDYAVQFACMVFVALPSLIIYLLLNKYITEGVTVGAIKG"
BASE COUNT          466 a          521 c          483 g          446 t
ORIGIN      
        1 taatcatcgc gataccccca ccggtaggat gggttgttgt tgaaagaacg atcctccccg
       61 gggtcgcgat agaataccgc catgatgtct tcgggaatcg cggcgccaat cgtttctaat
      121 gccgttccgt acttggcctc gagacccttc ccccaccatt tatgaaaaac caacgggatc
      181 ctggtaccgc ctttccgttc gatggcttga atcacttctt gcttggatag gggttctcga
      241 atcacgacgg ctcacccttt catgttagct tttggtcccg ctcaatccgc gatcaagatc
      301 gagttgacgg agtgaggctg taattcaaaa ttgtaaaccg catcatctat tgccaagcag
      361 agcagagcct ggtcgccaaa gggattccgg atcaccacgg tccttcccgc agccgagcga
      421 aaggcgaccg cattggcaga ccagggtttc accagtccca accggtgatc gccggggtgg
      481 atgaacttgg agaaatgttt catgatataa tattccggat tccgggtaat ccggcccgtt
      541 ttcggatcga cggaaacgag ggagttctgc tgccaacccc aggtgctttc gccgcctgtc
      601 tccagaacca tattccagta tacataggca atgacgtcgt tggtcagata gtggtaaaaa
      661 agatcgaaaa cataagctgc gtactcccaa gaattattgc cgtcaccgca ctcattctcg
      721 gtttgaatga cggggatctc cggccagccc tgccgggtcc gctgcaccgc atatttaccg
      781 cgccactgat atccgacgcc gccaatgtac tttctggcat gaggatcgct caacaccaaa
      841 ttggcgtaat cattataagt cgtacggaga taacgttcat caacctcggg cccgttgatg
      901 gttcccagcc aaacctcggt ggtaacccgg tgcttctcaa acagcggccc cagataatcg
      961 cggataaaat cccgtagttg ggcccctgtc cagatacagg aaggaatctt ctgatccgaa
     1021 accggttcgt tctgaacgtg cagttgttga atagggatcc caagcgcggc gtaactttgg
     1081 acaaacttca agagatagag ggcataagcc gctaaaaccg gcctctccca gataagcgtc
     1141 ccgaaattat aggcgcgagg gtatttcatc caggtcggcg gggaccaggg tgaagcaaag
     1201 agtctaaggc tggggcaaag gttttgggcg ctttgaatat acgggattaa actttgccgg
     1261 tcccgttcga tggagaaatg ctccatcccc aggtcacctt cggtctcgtt caggctgtac
     1321 caggaggagg cataatcgct cgcccccacc ggcacccggc agaagttgaa ttggcatccg
     1381 tagccgggct cgaataagtc ccgcaaaagt tcttgccgcg catcggaatc aagcgcctga
     1441 agcgcctgcc agccaagttc gttgaaacaa ccaccgaatc ccatccattc gggatggggt
     1501 tcatccacta tcgccagatt ggcctcgccc tgctcactac ggtgaaactt cctgccggga
     1561 acccattgct ccctttgatt ggtataaacc cattgaatct gcttcattgg taattcccct
     1621 ttgcctgaca tctaaccctt gatggctcct acggtcacac cctcggtaat atatttattc
     1681 aataataagt agataatcag ggagggtaac gccacaaaga ccatacaggc gaattggacc
     1741 gcgtaatccg atacatactc cccggtgaac ttgtaaaccg aaaaaggcag ggttcgcaga
     1801 ttgtcggtac ggatgaaggt atttaccatg atgaactcgt tccagttatt gatgaaggtc
     1861 atgatggccg tggtgatgat ggccggtttc gccacaggcg ccacgatctt aaacat
//
LOCUS       DASVMB010000489         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_206512,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000489 DASVMB010000000
VERSION     DASVMB010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206512"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..928)
                     /locus_tag="VF531_13735"
     CDS             complement(74..928)
                     /locus_tag="VF531_13735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7715070.1"
                     /translation="MKKIWIFGGLVVAVLMVGLVIFQVLAKKQESKAAAPKPTESPKA
                     EQSGDIEAFGTVKANNYKDIYLDFPARIVKLHVTEGQGVAFGQTLVTLDLVDFEAQIK
                     SKSLELNNARYELAQAEKNEQEAAEVYARAKQDLVAQEISNKRDKITLLTSELNRMQS
                     KLNQSYMKGNIIQSDLKHGVVYELGYTAGDPVSVEKKVLTIASLDNMVVDADVPEEFI
                     KDVRIGAKVTILPAADNDRKYEGRVIKIANMALTQNGETVIPVEISIVNSDGFLRPNF
                     NVDVTIEK"
     gene            complement(925..1599)
                     /locus_tag="VF531_13740"
     CDS             complement(925..1599)
                     /locus_tag="VF531_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715071.1"
                     /translation="MKVIEATDLCKTFTLGEVEVEVLNHINLTIDEGEFVSIMGPSGS
                     GKSTLLYLIGGLDKPTTGSIKIKGTELSSMKDAEESIMRRRDLGFVFQFYNLIPNLNV
                     EENIMLPILLDGKKMRSYKERLDEVLEIVGLTDRREHTPRELSGGQQQRVAIARALIN
                     EPDIILADEPIGNLDSKTGTEVMKLLKQINREHGKTIVQVTHSEEAAGYGERIIHVRD
                     GKVWES"
     gene            complement(1628..4129)
                     /locus_tag="VF531_13745"
     CDS             complement(1628..4129)
                     /locus_tag="VF531_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7715072.1"
                     /translation="MGIIIKFVLKNIKEKKFRTFLILFSIMLSAALYFASSAISGTVA
                     EMYTQRIRTYYGNADIVIHAGEKSPGRFVTAARAAEFGGRMEYIIGAVEGSGTYKYQN
                     ESVNLNLKGFTLDELQLNNPILLDNEDQAMDLRALEPFIGKKVIISKNLAQKYQLNPG
                     DHLDVEIQGAKHRFIIAGLAQPAGIFQDDGRSNVLLMPRNTLGAIYAARGKSTIIYLK
                     LKNPAELQQTLTDLSRSYRRYVVREPFSKAELLQNTSMITTPFMLMVSLVLLMSIFII
                     YTAFKVIMRERLPVIGTFRSIGATRRTTNTILFAESLLYGIIGGISGCILGIGILYAM
                     TLLIAGRMTGIPINISFNIGQMIAAFLLAIVLAAVSSAVPIIKTSKIPVKDIVLNKLE
                     KAHKKKLSRVPLGVVMLVATIIVPYYTPRTLALPIDMLCMIGAILAVIMLIPYFTGGF
                     IKLFERIYGLFGNEGVLAVKNLRGNRSILNNISLLAIGISSLLMINTVSFSVERLVVN
                     AYRDFKFDIWMGIWPADRRMESILRTVDGVKDVYGFYGANSVELADRPERIYWINGVN
                     PAKFADYMEVNLIGNSRDLLAELDQDRNILISSTLREKYGVKKGDWLTLKMKRGNKSY
                     KIIGFFNSVMENGSYALIAERYLKTDMMLRDYSEIFVKTAGDPGVVAQAIQKKFQRQR
                     PWLMTIPDMEKRNMQSSYNMFLILRGFSVLAMLIGIIGVLNNLLISFLERQRSLAVMR
                     SVGMSKRQEIKMILIESLTCGLIGGVIGVLGGILLIQVVPQLCQAIDLPLSITYAPSL
                     FVYSLIGGIIITVSATMSPAFKSARANIIEAIKYE"
BASE COUNT         1147 a         1126 c         1049 g         1240 t
ORIGIN      
        1 ggcttataag ccctaatatt tagttgtttg gaaggacact caaggacgga accttcaatc
       61 caatgggctt ttgttatttc tcaatcgtca catccacgtt gaagttgggt ctcaggaagc
      121 catcgctgtt cacgatggag atttccacgg gaatgaccgt ctcgccgttt tgcgtcaagg
      181 ccatattggc gatcttaatc actctaccct catatttgcg gtcattgtcc gcggccggca
      241 agattgtgac cttggctccg atccgcacgt ccttgatgaa ttcctcggga acatcggcat
      301 caactaccat attatccaag ctcgcgatgg tcaatacctt cttctcgacg ctcaccggat
      361 cgccggcggt atatcccaac tcgtagacga ctccatgctt aagatccgat tgaatgatat
      421 tgcccttcat atagctttga ttcaatttgg actgcatccg gttcaattcc gaggtgagca
      481 aagtgatttt atcccgcttg ttggaaatct cctgagctac cagatcctgc ttggcccggg
      541 cataaacctc ggcggcctcc tgctcattct tttccgcttg agccagctcg taacgggcat
      601 tgtttaactc aagggattta ctcttgatct gagcttcgaa atcaaccaaa tccaaagtca
      661 ccaacgtttg accaaaggca actccttgcc cttccgtgac atggagcttg actatccggg
      721 ccggaaaatc aagataaata tccttataat tgttggcctt cacggttccg aaagcctcaa
      781 tatctccgct ttgttccgct tttggagact cagttggctt cggtgcagcg gctttgctct
      841 cttgcttctt tgccagcacc tgaaatatga ccaagccgac catgaggacc gcaactacca
      901 aaccgccgaa aatccaaatt tttttcatga ctcccatacc tttccgtcgc gaacatgaat
      961 gattcgttct ccgtaccccg ctgcttcctc ggagtgagtt acctgaacga tggtcttgcc
     1021 atgttcacga ttgatttgtt tcaagagttt catcacttcg gttccggttt tactgtccag
     1081 attaccgatg ggttcatccg ccagaatgat atcgggttcg ttgattaatg cacgggcaat
     1141 ggctacccgc tgttgttgtc ccccggagag ttcccggggc gtatgctccc gccggtccgt
     1201 caggcctaca atttctaaaa cctcatccag ccgttcttta taagaacgca tcttttttcc
     1261 atcaagcagg atggggagca tgatgttctc ttccacattg agattgggga tcaggttata
     1321 gaattgaaag acaaatccta aatcacgacg gcgcatgata ctctcctcgg catctttcat
     1381 gctggagagt tcggttccct tgattttaat gcttccggta gtcggcttat ccagcccgcc
     1441 aatgaggtaa agcagggtgc ttttacccga accggacggg cccatgatgg acacaaactc
     1501 accctcatca atcgtcagat tgatatggtt caatacttcc acttcgactt cgcccagtgt
     1561 aaaagttttg catagatcgg tcgcttcgat gactttcatg aagagagttc acctcaactc
     1621 ttggttgtca ctcgtatttg atggcctcga tgatgttggc ccgggccgac ttaaaggccg
     1681 gactcatcgt ggcggatacg gtaatgatga ttcctccgat taatgaatat acaaacaggg
     1741 atggcgcgta agtgatggat aacggcaggt cgatggcctg gcacaattgc ggtacgacct
     1801 gaatcagcaa gattccgccc aaaaccccga tgacgccgcc gatcaaaccg caggtcaaag
     1861 attcaataag gatcatcttg atctcctgtc gtttgctcat tcccaccgaa cgcattaccg
     1921 ccaaggaacg ttgccgttcc agaaaactga tgagcagatt atttaaaacc ccgatgatcc
     1981 cgattaacat ggcgagtacc gaaaaaccgc gtaatatcag gaacatatta tagctggact
     2041 gcatattccg tttctccatg tcagggatgg tcatcaacca cggccgctgc cgttggaact
     2101 ttttttgtat agcctgcgcg accactcccg gatctccggc ggttttaaca aagatttccg
     2161 aataatccct gagcatcatg tcggttttta gataccgttc ggcgatcagg gcataactgc
     2221 cgttttccat cacagagttg aaaaacccaa tgatcttata ggacttattg ccccgtttca
     2281 tcttaagtgt cagccagtcg ccctttttaa ccccgtattt ttcccttaag gtgctggaaa
     2341 tcaagatatt ccggtcttga tcgagctcag ccaagagatc ccgggaattg ccgatcaggt
     2401 tgacttccat gtaatcggca aacttggccg ggttgacccc gttgatccag taaatccgtt
     2461 ccggccggtc cgccagttca acactgttgg ctccataaaa accgtaaacg tctttaaccc
     2521 cgtctacggt acgtaatatg ctttccatcc ggcgatccgc cggccagatc cccatccaga
     2581 tatcaaattt aaaatcgcga taggcattga ccactaaccg ttccacgctg aaactaaccg
     2641 tattgatcat caaaaggctt gagatgccga tggcgagcag tgagatatta ttcagaatgc
     2701 tccggttgcc ccggagattt ttgactgcca ggaccccttc gttgccaaac aagccataaa
     2761 tccgttcgaa caatttgatg aaaccgccgg taaaataggg aatcaacatg atcaccgcaa
     2821 gaattgcccc aatcatgcag agcatatcga tgggtaaggc cagggtccgg ggtgtatagt
     2881 aagggacgat gatcgtagcc actaacatga cgacgcccag gggaacccgg gagagcttct
     2941 ttttatgggc cttctccaac ttgtttaata caatgtcctt cacgggtatt tttgaggttt
     3001 tgataatcgg cactgccgag ctgaccgctg ccagaacgat ggccaaaaga aatgccgcaa
     3061 tcatttgacc gatgttaaag ctgatgttga tgggtattcc ggtcatccgg ccggcgatca
     3121 gtaaggtcat cgcgtatagg attccgattc ccaaaataca accgctaatc ccgccaatga
     3181 tcccataaag caggctttcg gcgaagagta tggtattggt ggtcctccgg gtagcgccaa
     3241 tgcttctgaa agtgccgatt accggaagtc tttcccgcat aatcacttta aaggcggtat
     3301 agataataaa aatgctcatc aaaagtacca gggaaaccat tagcataaat ggcgtcgtaa
     3361 tcatgctggt gttttggagg agttccgctt tggagaaagg ttcgcgcacc acataacgac
     3421 ggtagcttcg ggagagatcc gtcagagttt gttggagttc cgccgggttt ttcagcttca
     3481 aataaatgat ggtggacttg ccccgtgcgg cgtaaatcgc ccccagagta ttccggggca
     3541 tcaataaaac gttggagcgt ccgtcatcct gaaagattcc ggccggctgg gcgagcccgg
     3601 caatgatgaa acggtgtttc gctccctgaa tctccacatc caagtggtcg cccggattta
     3661 gctgatattt ttgggccagg ttcttgctga tgatcacttt ttttccgata aatggctcaa
     3721 gcgctcgcag atccatggct tgatcttcgt tatcgagcaa tatgggattg ttcagctgta
     3781 attcatcgag agtgaaacct ttaagattca ggttgacgct ttcgttttga tatttataag
     3841 tcccgctgcc ttccactgct ccgatgatgt attccatccg cccgccaaat tcggcggcac
     3901 gggcggcggt tacaaaacgt cccggtgatt tttccccggc gtggatcacg atatccgcgt
     3961 tgccatagta cgtccgaatc cgctgggtat acatctcggc gactgtcccc gagattgcgc
     4021 tggaggcaaa gtaaagcgcg gcggagagca tgatcgaaaa aaggattaaa aaggtcctga
     4081 atttcttttc tttaatattc ttcaaaacga atttaataat gattcccata aaggcaccac
     4141 cgtgaccgtg tcatgacaac aattgatttt aaacccgcgg gtaccaccaa ttggcaaaga
     4201 gtataacgaa gtttggatcc aatttgtttc aatgccgtga aagagtaatc gtagggatga
     4261 acttttttgc cacagatacc tggtaaatta tagtacaata attccaacca gcggtaaaat
     4321 cccctttaga atttttgtaa agatgatttt aaaagtggat gaacgggttg atgggtggta
     4381 atcggtaaag gcgggacaag taaagaagct tttgatggat aaaattagct cctttgccat
     4441 acccattttc gtgtcttttg tgtgtttcgt tgtaaataaa aaagggtgct tttatggagt
     4501 ttagtcaata gtccatcaca attgtaaaat gtaaattgca ggaatttaat aaagctcata
     4561 ga
//
LOCUS       DASVMB010000490         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_59489,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000490 DASVMB010000000
VERSION     DASVMB010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59489"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /gene="fusA"
                     /locus_tag="VF531_13750"
     CDS             <1..1957
                     /gene="fusA"
                     /locus_tag="VF531_13750"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7715073.1"
                     /translation="DGTATMDFDPEEIKRKISIGTGLAPLEYRDHKINLLDTPGFFDF
                     VGEVKGASRAADAACIVVCAVSGVEVGTEKAWSYADERGLSRVMIINKMDRENADFDG
                     AVKALRARFGNTIIPVQIPIGAAETFQGVVDILKNKALTLEAGKMVEQPIPANLAAAV
                     EEYRMMLVESVAETDDELLTKYLDGKELSDAELLTGARKGTIAGKLVPVLAGSALKLI
                     GITNILDVLLDFLPSPSDRGEMVGVNPNTKAEEKRKPAENEPFSALVFKTTADPFVGK
                     LTQFRVCSGVIKSDTQVFNSNKGTMERIAQLFVTKGKTQEPVSELGPGDIGAVAKLQE
                     TFTGNTLCDKDKPILYPPIDFPAPKLSLAVEPKAKGDEDKISSGLTRLMEEDPTFTVN
                     KDKVTHEIVASGMGDLHVEVITSKLHKKFGVEVTLKSPKISYKETIKSKVKVEGKHKK
                     QSGGRGQYGHVWLELSPLSPGSGFEFVDKIFGGAVPRNFIPAVEKGVRETMDRGVLAG
                     YPVVDVQVSLVDGSYHPVDSSEMAFKIAASLGFKKGFMDAKPILLEPILNVEVRVPEE
                     YMGDTIGDLNKKRGKILGMEPDGKDQVVRALAPQTELARYAIDLRSMTQGRGDFSVSF
                     DHYEEVPAQLAETIIAEARKHMTQEEE"
     gene            2162..2662
                     /locus_tag="VF531_13755"
     CDS             2162..2662
                     /locus_tag="VF531_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715074.1"
                     /translation="MKFRMAMVTAVVLTFLVSVTAYASIGGDNYVWVSEDLKGEYSKI
                     GYFRQDQMFTKIISTNSKDNVNSFDLSIGNRMISFDNSSGLYGELGVNSGSSKLNLFF
                     GAGYTYQSSDSDVTVMIGPKYYIQDQKLVTEAQAFVRILPPLVLSLGYDDNSKAIFVG
                     AGLRFE"
     gene            2934..4742
                     /locus_tag="VF531_13760"
     CDS             2934..4742
                     /locus_tag="VF531_13760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715075.1"
                     /translation="MCKPIKVIKPLNWIFIPQLKLRGKTLLLYGSVVILPIVLLGFLA
                     GHWILFSLRTDYQHSVHEAVNQVVKNVEFRKEIYETHILQAAYDRELINRLARRYDNM
                     KQKWDTVRYIDQSFGSARYILPGLTRCRVYHDSTTLPEDGGFVWKPARRMLGKYTEED
                     WYQRIAKRPDPLQWETLYNNESASYDLTLSQPILDSSEKLVGVVWMQLSGQQVFDALT
                     KEAFGGKGRLFLADANGVVFASTDNRYLGKEVKNTKLNMITLNKPSFQRTPESELVVV
                     QRIPSGWYVIGSVSTSVLEKQTRRVYTLVIVITLSLCIMSILLMFFFIDNFTKRLKRL
                     GSHMTALGQGDFEMTISREDADELGDLEEHFSRMARRLRILMEEIAYTRIKQREEALK
                     ALQAQINPHFLYNTLGMVRWRALDAGDQELCRIVDAMTTFYRLALNEGKSVLKVKDEI
                     NHVKAYLEIMQYRYPDKVEVYWDVDPETLDCYVIKLILQPLVENSFAHGLIHKQANGC
                     LWIKVSCRNDRIELSIRDNGVGMDPETITRILSGNYIQKGHGYGIYNIIERLRLCFGD
                     RGVLTISGTPGNGTTVTIQIPVCLEPSTVERGSQNA"
     gene            4735..>5517
                     /locus_tag="VF531_13765"
     CDS             4735..>5517
                     /locus_tag="VF531_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715076.1"
                     /translation="MLKALIVDDEPAHRIGLSNHLNWLELGYDPPVLAEDGKEALTLA
                     RESQIDVLITDICMPEMDGFEVIREIRATHPQIPVLIISGFEEFEFARAAVEAGAQAY
                     LLKPLRPEEIERWLIKFRDDILRRRAIAMEDQRFQTQFQASLAWAREKFLETLLAEKQ
                     SNHETAYSQAALLNLPVSGFRYYFLILSLDDYPALFAVRPDTASLIMFRVVNAVQVAL
                     KDCGEVIVGRLHPDEAVLCLIYPGEYIPTPEDLHNRLRDVQHT"
BASE COUNT         1443 a         1199 c         1404 g         1471 t
ORIGIN      
        1 tgatggtacg gcaacgatgg attttgatcc cgaggagatt aaacggaaaa tcagtatcgg
       61 tacgggtctc gctcctttgg aataccggga tcacaagatt aatctcttag atacgccggg
      121 attttttgat tttgtcggtg aagtaaaagg agcaagtcgg gccgcagatg ccgcttgcat
      181 agtggtttgc gccgtttccg gtgtagaagt tggaaccgaa aaggcatgga gttatgctga
      241 tgaacggggt ttatcccgtg taatgatcat taataaaatg gaccgggaga acgccgattt
      301 tgacggggcg gtgaaagcgc tccgcgccag attcggaaat actatcattc cggtacagat
      361 ccccatcgga gcggctgaaa cgtttcaggg cgtggtggat atattaaaga acaaggctct
      421 tacccttgaa gccgggaaga tggttgaaca accaatcccg gccaacctgg ctgcggctgt
      481 ggaagagtat cgtatgatgc tggtcgaatc ggtggcggag accgatgatg agttgctcac
      541 caaatatttg gatggtaagg aactatctga tgccgaatta ctcaccggtg ccagaaaggg
      601 caccatcgcc ggtaaactcg ttccggtttt ggccggttcg gcgttgaaac tgattggcat
      661 caccaacatt ctcgatgttt tattggactt tttaccttcg ccatccgatc ggggcgagat
      721 ggttggagtc aaccccaata cgaaggcgga ggagaaacgg aagccagctg aaaatgagcc
      781 gttttccgct ttggtcttta agacaacggc cgatcccttc gtgggtaagc tgacgcagtt
      841 ccgtgtctgt tccggtgtga tcaaatccga tacccaagtc tttaattcca acaaaggtac
      901 gatggaacgg attgctcaac tctttgtgac caagggaaag actcaggaac ccgtttcgga
      961 gctgggacct ggtgatatcg gcgctgtagc caaacttcag gagactttta ccggaaacac
     1021 cttgtgcgat aaagataagc caattttgta tccgcccatc gattttccgg cccctaaatt
     1081 atctctagcg gtagaaccca aggccaaagg cgatgaggat aagatctcca gcgggctgac
     1141 tcggttgatg gaggaagatc ccacctttac ggtaaacaaa gataaggtta cccatgagat
     1201 cgtcgcctcc ggtatgggag atcttcacgt tgaggtcatc accagtaaat tacataaaaa
     1261 attcggggta gaggttaccc tgaaatctcc gaagatatca tataaggaaa cgatcaagtc
     1321 caaggtgaag gtggagggta agcacaagaa acagagcggc ggtcgcggcc aatacggtca
     1381 tgtatggctc gaactaagcc ccctttcgcc ggggagtggt tttgagtttg tcgataagat
     1441 tttcggcggc gcggtacccc gcaatttcat tcctgcagtt gaaaaaggcg ttcgcgaaac
     1501 gatggaccgg ggagtacttg ccggttatcc tgtagtggac gttcaggtca gtttagttga
     1561 cggttcttac catccggtgg actcctccga aatggctttc aagatcgcag cttcactggg
     1621 atttaagaaa ggcttcatgg atgccaagcc gattctgctt gagcccatct tgaacgtgga
     1681 agttcgggta cccgaagagt atatggggga taccatcggt gatctcaata aaaagcgcgg
     1741 caagattctg ggtatggaac cggacggcaa ggatcaggtg gtgcgcgccc tggcccctca
     1801 gactgaactt gccaggtacg ccattgatct tcgttccatg acccaaggtc gtggtgattt
     1861 ctccgtaagc ttcgatcatt acgaagaagt tccggcgcaa ttggctgaga ccattatcgc
     1921 cgaagcccgg aaacatatga ctcaggaaga ggagtagccc tggcgcatac gcctggacta
     1981 cggcattgta tgaaattctt agccgacctt tcaggtcggt ttttttgtgt caagggttat
     2041 atgaaccgtt tgtaaagacg gtgtcagttt attttctctt tatttacccc aactttacta
     2101 aagagatacc cggaatgcga cgataatata taggaatcta catccggaaa ggggttctgt
     2161 catgaaattt agaatggcaa tggtcacagc agttgtcttg acctttttag tttccgtcac
     2221 cgcctatgct tccattgggg gggacaatta tgtatgggtc agtgaggacc tgaaaggcga
     2281 atactccaaa atcggttatt tccggcagga tcagatgttt accaaaatta tctccaccaa
     2341 ctcgaaggat aacgtcaatt cttttgatct ctccatcggc aaccggatga tctcttttga
     2401 caatagttcc ggcctatacg gggaattagg cgtcaattcg ggttcgagca agttgaatct
     2461 cttttttggc gcaggttaca cttatcaaag ttcggattcg gatgttacgg tgatgatcgg
     2521 gccgaaatat tacatccagg atcaaaagct ggtcacagag gcgcaggcat ttgtgagaat
     2581 tcttccacct ttggttttaa gcctcggtta tgatgacaac agtaaggcga tctttgtagg
     2641 agcgggttta cgctttgagt gattgtatac ctgttttcca taatgtaaat tgttcgcctc
     2701 ggcgtgaggc gatttttttt tgctccgatt tttcctttcc tcatcttctt gcaacatatt
     2761 ccgcattttt ggttcaatga caatttacac tccaggcagg aagtttttca ggtatttcga
     2821 attattgggg tatatgggtg gaagatgtga tagagcgtgg ctatacatgc gctaccacaa
     2881 agcacctacc tgaaaccggg atacttggaa tttaaacgga tggggtcaaa agtatgtgta
     2941 aacctatcaa agttataaag ccgttaaatt ggatattcat tccccaacta aaattaagag
     3001 ggaaaacact gctattatac ggtagcgtcg ttattctccc cattgtcttg cttggttttt
     3061 tggcagggca ttggatttta ttctcattgc ggactgatta tcaacattca gtccatgaag
     3121 cagtaaacca ggttgtcaag aacgtggagt ttcgtaagga aatatatgaa actcatatat
     3181 tgcaggcggc gtacgatagg gagttaatca accgactggc acgccggtat gataatatga
     3241 aacaaaaatg ggatacggtt cgatacattg accagtcctt tggttcagcc cgttatattc
     3301 tgcccggatt aacacgttgc cgtgtttatc atgactcgac aaccttaccc gaagatggag
     3361 gttttgtctg gaaaccagcc cgtcgtatgc ttggaaaata taccgaagaa gattggtatc
     3421 aacggatcgc caaacgaccc gatcccttgc agtgggagac actttacaac aatgaatccg
     3481 ccagttacga tttaaccctg agtcaaccga ttttggactc ttctgagaaa ctggtaggag
     3541 tcgtctggat gcagttaagc ggtcaacagg ttttcgacgc cttgaccaaa gaagctttcg
     3601 gaggaaaagg ccgtttattt ttggctgacg cgaatggggt ggttttcgcg tccacggaca
     3661 atcgttactt gggtaaggaa gttaaaaaca caaaattaaa tatgattacg ctaaacaagc
     3721 catcctttca acgaaccccc gagtccgaac tggtagttgt tcaaagaatc ccttccggtt
     3781 ggtatgtcat tggttcggtc tccacaagcg tcctggaaaa acaaacccgt cgggtttaca
     3841 cgttggttat tgtcattacc ctgagcctct gcatcatgtc gatattatta atgttctttt
     3901 ttatcgacaa ttttacaaaa cgcctgaaac gtcttggttc acacatgacg gcgcttggcc
     3961 aaggggattt cgaaatgacg atttcccggg aagatgctga cgaacttggt gatttggaag
     4021 aacacttttc aagaatggcc cgccggttga ggattctaat ggaagaaatt gcttacacgc
     4081 ggattaagca aagggaagaa gccttaaaag cgttacaggc acaaatcaat cctcattttc
     4141 tctataacac gctcggcatg gtccggtggc gggctcttga cgccggagat caggagttat
     4201 gccggattgt cgatgcaatg accacatttt accgtctggc gctgaatgag ggaaaaagcg
     4261 ttctaaaagt aaaagacgaa atcaatcatg tcaaagcgta tttggaaatt atgcaatacc
     4321 gttatccgga taaggtggag gtttactggg atgttgatcc ggaaacgctt gattgttacg
     4381 tcattaaact gattctacag cctttggtgg agaactcatt tgcccatggt ttaattcata
     4441 aacaagcgaa cgggtgttta tggattaagg tgagttgccg taatgatcgg attgagctat
     4501 ccatcaggga caatggagtt ggtatggacc ccgagacaat tacccgaatc ttatcgggaa
     4561 attacattca aaaaggccac ggatacggca tttataatat cattgaacgg ttgcggcttt
     4621 gtttcggaga ccggggcgtt ttaaccattt ccgggacccc cggaaacgga acaactgtga
     4681 caatccaaat tccggtctgc cttgagccgt cgaccgtaga aagagggtcc caaaatgctt
     4741 aaagcgttga ttgtcgatga tgagcccgcg catcggattg gactttccaa tcatcttaac
     4801 tggcttgaac tcggttatga tcctccggtt ctggctgaag acggcaagga agccctgact
     4861 ctcgcgcgag agtcccaaat cgacgtatta attacggata tctgcatgcc ggaaatggac
     4921 ggctttgaag tcatccgcga aattcgcgca actcatcctc aaattccggt cttaatcatc
     4981 agtgggtttg aagaatttga atttgcccgg gctgcggtgg aggccggcgc ccaggcctat
     5041 ctgcttaaac cgcttcgccc tgaggagatt gaacgatggc tcatcaaatt tagagacgat
     5101 atcttgcgac gcagggcaat tgccatggaa gatcagcgct tccaaactca attccaggca
     5161 agtctggctt gggctaggga aaaatttttg gaaacactac tggccgaaaa acaatccaat
     5221 catgagacgg cgtattctca agctgcttta ttaaacttac cagtgagcgg gtttcgttat
     5281 tattttttaa ttctttcact ggatgattat cctgctctat tcgcagtaag accggacact
     5341 gcctcactga ttatgttcag agtcgtcaac gccgtgcagg tcgccctgaa agactgcggg
     5401 gaagtcatcg ttgggcgtct tcatcccgat gaggccgtgc tttgcctgat ttatccgggt
     5461 gagtatattc cgactccgga agatctccat aaccggctgc gggatgttca acatacg
//
LOCUS       DASVMB010000491         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_356970,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000491 DASVMB010000000
VERSION     DASVMB010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356970"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF531_13770"
     CDS             complement(<1..950)
                     /locus_tag="VF531_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7715077.1"
                     /translation="MKRLFPVLFITCLVLSLLFAAIGVQAKSPVEISYYLWDDPTYRQ
                     IVDAFNSSQKEVLVKATYLPAADYETKLLTLLAGGVSMDAYMQKRQADMFPQYANGYI
                     EPLDKLIKKYKFNIAADKAYMSDISVNGKVVAIPFRGAGYFTYYNKKVFEKAGIPTPT
                     EYVKKGEWTWSKFIEVSKKLSTGDGKQYGAIFYTWPVNTFLPAYQQRVNFISANGKID
                     VDIDSVLFSMRMRRDLEKSKAIIPLAELKATKTHYSQAFYQGNVGMLLIGEWFPQFML
                     AARDQKLLKDFTWNDWAITRIPCDSSNYITAGASTFNHIYS"
     gene            complement(1148..1813)
                     /locus_tag="VF531_13775"
     CDS             complement(1148..1813)
                     /locus_tag="VF531_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715078.1"
                     /translation="MDKIKVLLVDDQTLFVESLRTVLQTLAKDILVVAVAYDGPRALQ
                     LVAEKHPDVVLMDVRMPGMNGVETTKLIKERFPEIQVLMLTTFNDDDYVVEALKLEAV
                     GYLLKDTPPAKLITAIRAVHDGGVLISQKVAAKVVTKLANTPEKDQDDETETSTAVFE
                     LSSREKEVLRLLAQGFENGEIASKLYIAEQTVKNHISNIYSKLGVHDRLQAYRKAKEI
                     GIG"
     gene            complement(1806..>2758)
                     /locus_tag="VF531_13780"
     CDS             complement(1806..>2758)
                     /locus_tag="VF531_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715079.1"
                     /translation="LITVIIEAIIFTRLREGLAFSFILMFFVIFFQNRMSVWNMALPA
                     ASLWDLLSMVISAMITVTLTILLRFQNNVSFRELNHRMNEATANLAEINLQLQEYAAA
                     AEQQAVINERKRFGSEIHDTLGYLLTNLVMMLEAAIVITPAKARKLRDHLRLTRDQAK
                     EGLFEMRRSLEKLRPIQSQVSGLLAISSLVNTLTRATHIKVDINFGDAPLNFGEKADH
                     AVYRLVQEGITNALRHGKATRIEISFGCKENGVNVWIKDNGIGTSEFEEGMGLTGMRE
                     RLEKLGGNLKLSSTVGEGFLLIAWLPMENSGGEHGEDSHG"
BASE COUNT          717 a          680 c          593 g          768 t
ORIGIN      
        1 gaataaatat ggttgaaagt ggatgccccg gccgtgatgt aattggaaga atcacacgga
       61 atgcgggtga ttgcccaatc attccaggtg aaatccttga gtagtttttg atcccgggca
      121 gccagcataa attgggggaa ccattcgccg atgagtaaca tgcctacatt gccttgatag
      181 aaggcttgag aatagtgtgt cttggtcgct tttaactcag ctaacggaat gattgccttg
      241 gatttttcca aatcccgcct catccgcatc gagaataaga cggaatcgat gtccacatcg
      301 atttttccat tggcggagat gaaattgact ctttgctgat atgccggcag gaatgtattg
      361 acgggccagg tatagaagat cgcgccgtat tgtttgccgt cgccggtcga gagttttttg
      421 gatacttcga taaacttact ccaggtccat tcaccctttt tgacgtactc ggtcggggtc
      481 ggaatcccgg ctttctcaaa aaccttcttg ttgtaatagg tgaaataccc ggcgccccgg
      541 aacggaatcg ccacgacttt gccattaacg gaaatgtcgg acatatacgc tttatcggcg
      601 gcaatattga acttgtactt cttgatgagc ttatcaaggg gttcaatgta accgttggca
      661 tattgcggga acatatcggc ttgccgtttt tgcatataag catccatact tactccgccg
      721 gccaaaaggg tgagtaactt ggtttcataa tcggcggccg ggaggtaagt ggctttgact
      781 aatacctctt tctgactgga gttaaaggca tcaacaatct gcctgtaggt tggatcatcc
      841 caaagatagt aactgatttc taccggggat ttggcctgaa cgccaatggc cgcgaacaaa
      901 agggagagaa ccagacaggt gatgaataaa accgggaaca gtcttttcat tccaaaatcc
      961 tcctttagtt ctttgtttga ataatcggtt cgaactccga ccgttaactt catcttaaca
     1021 ttttgggttc gcgaaaactt gattcgcaag tactgttttc agatcattct tgctagtact
     1081 ggagtaccgg taataaaaaa accatcccaa agcagatggt ttagcgggta atttcatgat
     1141 agatccatta tccaatcccg atttccttgg ctttccggta ggcctgaagc cggtcatgca
     1201 ctcccaattt agaataaata ttgctgatat gatttttaac cgtttgttcg gcgatatata
     1261 atttggatgc aatttcgcca ttttcaaaac cctgcgccag taagcgcaag acttccttct
     1321 ccctgctgct cagttcaaaa accgccgtac tggtctcggt ttcatcatcc tgatctttct
     1381 caggagtatt cgctaatttc gtaaccactt tagccgctac tttttgtgag atgagaaccc
     1441 cgccatcatg aaccgcccgg attgcagtaa tcaacttggc cggaggcgtg tctttcagca
     1501 aatatccgac tgcctcgagt tttaaggctt caaccacgta atcgtcgtcg ttaaaagtag
     1561 tcagcatcaa tacctggatc tcgggaaacc tttccttgat taatttcgtc gtttcgactc
     1621 cattcatgcc cggcatcctg acatccatca gaacaacatc cggatgtttt tcagccacca
     1681 attgcaaagc cctgggaccg tcgtaggcca cggcgacaac gaggatatct ttggccaacg
     1741 tttgaagtac tgtgcgtaaa ctctctacaa aaagagtctg atcatcgacc aagagcactt
     1801 taattttatc catgcgaatc ttctccatgt tcgcccccgc tgttttccat aggtaaccac
     1861 gctatcagta aaaagccttc acccaccgta ctcgataact tcagattgcc gcccagcttt
     1921 tcaaggcgtt ccctcattcc ggtcaacccc ataccttcct caaactcgct ggtgccgatc
     1981 ccgttatcct taatccaaac attaacgcca ttttctttac agccaaaaga gatctcaatc
     2041 cgtgtggctt tcccatggcg caacgcattg gttatcccct cttgaaccaa acgataaacc
     2101 gcatggtcgg ctttttcgcc aaagtttaac ggcgcatcgc caaagtttat gtcgaccttg
     2161 atatgagtag cccttgttaa ggtattcacc aagctgctga ttgccaaaag cccgctgact
     2221 tgtgattgaa tcggacgcag cttttccagg gaacgacgca tttcgaacaa cccctctttg
     2281 gcctgatccc gggttaaacg cagatgatcc cgtaacttcc gggctttggc gggggttatc
     2341 acgatggcag cttccagcat catcaccaga ttggtcagca aataacctaa ggtatcatgt
     2401 atttcacttc cgaaccgttt cctttcgttg ataaccgcct gttgttcggc tgcagccgca
     2461 tactcctgca attgtaggtt tatttcggcc aaattggcgg tggcttcatt catacgatga
     2521 tttagttccc taaaagatac attattttga aagcgcagca gtatcgtaag ggtcaccgta
     2581 atcatcgcgg aaattaccat ggataacaaa tcccataggg acgcggccgg taaagccata
     2641 ttccatacgc tcatccgatt ttgaaaaaag atgacgaaaa acattaatat aaaagaaaaa
     2701 gccagaccct cccgtaagcg ggtaaagata atggcttcaa taatcaccgt aatgagca
//
LOCUS       DASVMB010000492         4678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_59762,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000492 DASVMB010000000
VERSION     DASVMB010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59762"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_feature    complement(20..74)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            295..1245
                     /locus_tag="VF531_13785"
     CDS             295..1245
                     /locus_tag="VF531_13785"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX7715080.1"
                     /translation="MRIGISNTLFGATHLPYWHHFFSFLGLDVVIPGPSCKETVDCGG
                     KLIPPEFCVPVKACLGQLVELIEKDVELILLPRMIQKHRRDFFCPKFTGLPELAKYSA
                     GIDPDRLFSPEVICDGWQLKPIRYPAFIKPLGKLKEAAEQAGLYWDEVLTRCRREHLI
                     LPDALNRKAVTEPTGIRLGLLGYSYALYDPFISKGMIHKLTSLGAELRTWEMVDPAII
                     ESNLKGLKRPLFWNFGRVLLGAGLDFLKDPEVDGVVYVTTFGCGPDSIAIKMLELEAG
                     ITHKPFLRITLDEHSGDGHLTTRLEAFLEMLVERKEEAHA"
     gene            1250..2335
                     /locus_tag="VF531_13790"
     CDS             1250..2335
                     /locus_tag="VF531_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715081.1"
                     /translation="MKVTFPYLGPVLAYKKLFEMLGHEVIMPPRPTKRTIELGVKYSP
                     EFICFPFKIILGSYLESVEQGVDLVVTSGGIGACRAVYYGNLSEQILKKLGYHPRFLV
                     FDNILSRFPDFYRQAQVIRDHHPLSRLLYSTYMALSLTYGLDRYEKTIHRLRPYESVT
                     GTCDQKWLEIRNLLSQSNTLPELSSSMRVADQMIAGIATRPNPDLLKIGLVGEIYLVM
                     EGSVNHELMSELALLGVEVNRYRYISDWISHSFWPPRRILSRADHYLRYPLGGHEREN
                     IGHIINYKELGYDGVIHLMPFGCMPELVTQAIIPTLSRDLDLPILSLSLDEQTGYLNN
                     RVRLEAFIDLLRNRRELLSRPSGIVRG"
     gene            2678..3649
                     /locus_tag="VF531_13795"
     CDS             2678..3649
                     /locus_tag="VF531_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX7715082.1"
                     /translation="MMTEKVFLGIDVGSVSTNIAALDETGDVIWSRYLRNDGQPLQVI
                     QEGLRNIRSEVPDLQIQGVGATGSGRLLAGSVLGADIVKNEITAHAAAAIHFYPKVRT
                     ILEIGGQDSKIIMIQNGVVVDFAMNTVCAAGTGSFLDHQAERLKIPITDFGDLALTAT
                     RKVNIAGRCTVFAESDMIAKQQFGFSKADIINGLCEALVRNYLSNLARDKELASPILF
                     QGGVAANRGIKAAFERELKASIIIPSYYDVMGAVGAALLARRWLRRLNKNSRFRGFDQ
                     LMADLNPSTFECEGCSNRCEVIRVEQDRRIIAIWGDKCGKYTNSISA"
     gene            3878..>4678
                     /locus_tag="VF531_13800"
     CDS             3878..>4678
                     /locus_tag="VF531_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715083.1"
                     /translation="MSPINGANNNHPAFFPINFDDSSKVSNENNNTQPNINSAGEDPD
                     FQIVIKSLLENRINSWCNSLNTSDPNSNPEPLFPLSLLPLIGTQNNTKTLLAILNQLQ
                     QSDIVSRYSNQINEQSLSRYTNLLKQMEGKITNTPEMIHDANSNLAPGINPSRPEIVD
                     YITEQCRELGIPEQLGLATATTESGMTQFNKDGTPCRNSNPSSSDWGIMQINDKAWGN
                     TYDMNRIKSDWKYNVRAGLEILRDCYNAAIKNNEDSKGVNSSDQNLARA"
BASE COUNT         1251 a         1110 c         1164 g         1153 t
ORIGIN      
        1 ggtggcaagc tcaaaaatga gccttccacc ccgaaagcct ggtttctctt gaaagcgggt
       61 aaccccgccc aaccgtaatg aaagagtatg aagtttcaac aacagctcta ttatatcatt
      121 tcaccggaaa agattaaacc ctatccggat ccggttgagg aaaaccatag gaaatacgag
      181 acggcgtttt cgcatgactt ccgacaaatt ctgccgcctt gcatagattc ataccaaaat
      241 gccaaatata ggagcagaat ataaaagaaa attcatttca tctggcagga aattatgaga
      301 atcggtatat ccaacaccct gttcggcgcg actcatctac cgtattggca ccattttttc
      361 agctttttag gattggatgt cgtcatcccc ggccccagtt gcaaggaaac tgtcgattgc
      421 ggcggcaaat tgatcccccc cgagttttgc gtaccggtca aggcctgtct cgggcagttg
      481 gtcgagttaa tagagaaaga cgtagagttg attctgctcc ccagaatgat ccaaaagcac
      541 aggcgggatt ttttttgccc taaattcacc ggtctgccgg aactggccaa gtattcagca
      601 gggattgatc ccgaccgcct tttttctccg gaggtgatct gtgacggctg gcagttgaaa
      661 cccattcgat atcccgcttt tataaagccc ctcggcaagc tcaaggaagc ggccgaacag
      721 gcgggcctat actgggatga ggttttaacc cgatgccgcc gggaacatct gatcttgccc
      781 gatgctttaa atcgtaaggc tgtcacggaa cctaccggaa tccggcttgg tttattggga
      841 tattcttatg ccctgtacga tccatttatc agtaagggaa tgatccataa actgacctcc
      901 ctcggagccg aactccgtac ctgggagatg gttgacccgg cgatcatcga aagtaatttg
      961 aaagggttaa aacggccttt attttggaat tttggacggg ttctgctcgg cgccggtctt
     1021 gacttcctaa aagatcctga agttgacgga gtggtatacg tcaccacttt cggttgcgga
     1081 ccagactcca tcgccatcaa gatgctggaa cttgaggcag ggataaccca taaaccgttt
     1141 ctacgaatca cccttgatga acatagcggc gacggacatc tcacgacccg gcttgaggca
     1201 ttcctggaga tgctggttga gaggaaagag gaggcgcacg cgtaagcgga tgaaagttac
     1261 ctttccctac ttaggcccgg tactggccta taaaaagctc tttgaaatgt tgggtcatga
     1321 agtgattatg cctccgcgcc ccaccaagcg aacaattgag ctgggggtga agtacagccc
     1381 ggaattcatc tgttttccct ttaagatcat cctcggaagc tatctggagt ctgtcgaaca
     1441 aggtgtggat ctggtagtga cgagtggggg tatcggggcc tgccgggcgg tttattacgg
     1501 taacttgagt gaacagatat taaagaagct gggatatcat ccccggttct tggtctttga
     1561 caatatcctg tcaaggttcc cggattttta tcgccaggct caggtaatcc gggaccatca
     1621 tcccctatcc cgacttttat actcgactta tatggcgtta agtcttacat atggactaga
     1681 tcggtatgag aaaaccattc acaggcttcg cccgtatgaa tcggtaaccg ggacctgcga
     1741 tcaaaagtgg ctggagattc gaaatctgct tagccaaagc aatacgctcc cagaattaag
     1801 ttctagcatg agggtggcgg atcagatgat cgcgggtatt gccacccgtc ccaacccgga
     1861 tttattaaaa atcggattgg tgggggagat ctatctggtg atggaaggat cggtcaatca
     1921 tgaactcatg agtgaattgg ctctcctggg agtggaagtc aaccgttacc gttatatctc
     1981 agattggata tctcacagtt tctggccgcc ccggcgaata ctgagtaggg cggaccatta
     2041 tctccggtat ccactgggag gccacgaaag ggagaacatc ggccatatca tcaactataa
     2101 agaactgggc tatgacgggg ttattcatct aatgcctttc ggttgcatgc cggagctggt
     2161 gactcaggcc atcatcccta ccctatcccg ggatctggat ttaccgattc tctccctgtc
     2221 actcgacgaa caaaccgggt acctcaataa ccgggtgcgc ttggaggcct ttatcgatct
     2281 attgagaaac cggagggaat tattaagtag accatcaggg attgtgaggg ggtaaaccta
     2341 tctcttaggg ctatctcaaa agtccaattt gtcattccgt ccacccgggg tgcgcgtccc
     2401 atgggacgcg agaaaccccg ggctaagtta aaaaccattc taaagcccgt taaaacgggc
     2461 tagggagaac cggatgtgtt acccagcccc attcatgggg cttgagatga tttttccggg
     2521 cgtagaaagg ctcctaaagg gcctggtgga gataaggacg attatccatt gtttgctttt
     2581 gagacaaccg caacctctgg ctttgtagtc aaagaaagga cagccttcgg ctccggagca
     2641 gttgagtatt tatgtgataa cgattggagc aatggatatg atgacggaaa aagtatttct
     2701 cggtatcgat gtgggttcgg taagcaccaa tattgccgca ctggatgaaa caggggatgt
     2761 tatttggtca agatatctca gaaacgacgg acaacccctt caagtgatcc aggaggggtt
     2821 aaggaatata cgcagcgagg tccctgattt acaaattcag ggagttggag ccacagggag
     2881 cggccgactg ctggcaggga gcgtattagg cgccgacatc gtgaaaaatg agattaccgc
     2941 ccatgccgcc gcggcaattc acttctatcc gaaggtccga acgatccttg agatcggagg
     3001 ccaggattcc aagatcatca tgattcaaaa cggggttgtg gtggacttcg ccatgaatac
     3061 ggtttgcgcc gcgggaaccg ggtctttcct ggatcaccag gctgaacggt tgaagatccc
     3121 tattaccgac ttcggtgatc tggctttaac cgcaaccaga aaagtcaata ttgccggacg
     3181 ttgcacggtt tttgccgaat cggacatgat tgccaaacag caatttggct tttccaaggc
     3241 ggatatcatc aatggtttat gcgaggctct ggtccggaat tatctgtcta atctggcacg
     3301 ggacaaagaa ttggcttcgc ccatcctttt tcagggcgga gtggcggcca accggggtat
     3361 caaagctgcc tttgaaagag agttaaaggc ttcaatcatc attccctctt attatgacgt
     3421 catgggggcc gttggcgccg cgctactggc acggagatgg ctgagaagat tgaataagaa
     3481 ttccagattt cgcggcttcg accaattgat ggccgattta aatccttcca cttttgaatg
     3541 cgagggatgc agcaaccggt gcgaagtgat tcgggtggag caagatcggc ggatcatcgc
     3601 catctggggt gataagtgcg gcaaatatac gaacagtatt tccgcttaaa tgttctacct
     3661 gcctcatcag ccccaagttt aaattcacgc agatggcggt ggctccggag agctgcctgg
     3721 ctatttttaa tgttcagtat tgggtgttcc atgatagaat agtttaggca atccttgcta
     3781 aattatttgc cgttttttcc gataaattat ttgacacact cgttagtcat tcttctctga
     3841 atccatccgg taagcctgtt aaggaggatg taacaaaatg agcccgatca acggcgccaa
     3901 caacaaccat ccagcctttt tccctatcaa ctttgacgac tcctccaaag tcagcaatga
     3961 aaacaataac actcaaccaa atatcaactc agcaggggaa gatcctgatt ttcaaatcgt
     4021 cataaagtcg ctcctggaaa atagaatcaa cagctggtgc aattcactaa ataccagcga
     4081 tccaaattcc aacccggagc ctttattccc cctgtcctta ttaccgctga taggtacgca
     4141 gaacaatacc aaaacactac tggccatact gaaccagttg caacaatctg atattgtgtc
     4201 acgatattcc aaccagatca atgaacaatc attaagccgg tataccaatc tactcaaaca
     4261 aatggaaggc aaaataacaa atacacctga aatgattcat gacgccaaca gtaacttagc
     4321 tcccggaatc aatccctcgc ggccagagat tgtcgactat attaccgaac aatgccggga
     4381 gttgggtatt ccggaacagc tgggtttggc tacggccaca accgaaagcg gcatgaccca
     4441 gtttaataag gatggaactc cttgccgcaa ttccaatcca agctcttcgg attgggggat
     4501 catgcagatc aatgataaag cctggggaaa cacctacgat atgaatcgga tcaaatcgga
     4561 ctggaaatat aatgtccggg ccggtttgga gatcttacgg gattgttata acgcagccat
     4621 caaaaataat gaagatagca aaggcgtgaa cagtagcgac caaaatctgg cccgcgcc
//
LOCUS       DASVMB010000493         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_206845,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000493 DASVMB010000000
VERSION     DASVMB010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206845"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF531_13805"
     CDS             complement(<1..379)
                     /locus_tag="VF531_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7715084.1"
                     /translation="MNTVTETELKSFPLVGRGKVRDIYDLHRYLLIVATDRLSAFDVV
                     FDDPIPDKGRVLTGLSVYWFQETASAFPNHLVSADLETVPGLTVAERSLLQGRSLVVK
                     KGQVIPVECVVRGYLAGSGWKEYQ"
     gene            complement(418..849)
                     /gene="fabZ"
                     /locus_tag="VF531_13810"
     CDS             complement(418..849)
                     /gene="fabZ"
                     /locus_tag="VF531_13810"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7715085.1"
                     /translation="MLDINQIKAAIPHRFPFLLVDRIIELEKGKRAVGIKNVTADDFF
                     SQAELSENGIYPGALQVEAMAQVAGFVLIDLLDQKEMVPLFGGIEYARFRKSARPGDQ
                     LRIEVNLSKFKIRTGKFEAKAYIGNELASEAHFTCVLGKES"
     gene            complement(863..2431)
                     /gene="purH"
                     /locus_tag="VF531_13815"
     CDS             complement(863..2431)
                     /gene="purH"
                     /locus_tag="VF531_13815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7715086.1"
                     /translation="MAKIQRALLSVSDKTGLVDLAKFLTSHGVELISTGGTYKALQEA
                     GLSVTYISDVTGFPEILDGRVKTLHPAIHGALLAIRDSAKHQQELAHERIQPIDLVVV
                     NLYPFAATIAKAGVTLEEAIENIDIGGPSMLRSAAKNHRDVTVVVDPADYPLLIQEIE
                     ANQGGTSLEFRKRCAVNVFRMTALYDSMIFNYFNTAYNQADKEYPDLLQLSFVKQQSL
                     RYGENPHQKAAFYRETSPRPGTITTARQLHGKELSYNNIGDGNAALELVKEFGEPAVV
                     AVKHANPCGVGTGATIFEAYQKAFESDPVSIFGGIIALNREVDLDTATPMSEMFLEII
                     IAPSFTGAAFDLLAKKKNLRLLEVPELAVNSGSILTPDLDMKKVAGGLLVQETDVITL
                     NPADLKTVTKRQPTPAELDELLFGWKVVRYVKSNAIVLTRNHGTIGVGAGQMNRVGSA
                     RIAIEQAGSKAKGSCLASDAYFPMPDTVEVAAQAGITAIIHPGGSLHDQDSIDMADRY
                     NIAMVITGIRHFRH"
     gene            complement(2477..3103)
                     /gene="purN"
                     /locus_tag="VF531_13820"
     CDS             complement(2477..3103)
                     /gene="purN"
                     /locus_tag="VF531_13820"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7715087.1"
                     /translation="MDIKRIGVLVSGGGTNLQSIIDAAVAGTIGAEIGVVISNRPDAY
                     ALERATRHGITTRVIDHHQFSSVTAFTRAILDAMRESRIDLICLAGFMRILDPLLIEA
                     YPGRILNIHPALLPAFGGRGMYGLHVHKAVLDSGARFSGATVHLVTAETDVGPIVTQD
                     VVAVADDDTPETLAAKVLQIEHRIYPEAIRLIVRDQVIVDGLRVRRKT"
     gene            complement(3115..>3457)
                     /gene="glyA"
                     /locus_tag="VF531_13825"
     CDS             complement(3115..>3457)
                     /gene="glyA"
                     /locus_tag="VF531_13825"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860191.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7715088.1"
                     /translation="VKLITNGTDNHMMVIHTVTSFGIDGKVAEQVLDRIAITTNKQII
                     PDDPNPPLRPSGIRLGTPAATTRGMKEAEMRRIAGWIVRALRAHQDEAVLAGLRGEVE
                     AFCSQYPVPGI"
BASE COUNT          774 a         1014 c          900 g          769 t
ORIGIN      
        1 cctggtactc cttccagccg ctgcccgcca gatacccacg gaccacgcac tccaccggaa
       61 tcacctggcc cttcttgact acgagcgacc tgccctggag caggctccgc tccgcaacgg
      121 tcaagccggg gactgtttcc aggtccgcgc tgaccagatg attaggaaaa gcggaggctg
      181 tctcctggaa ccagtaaacc gagagtccgg ttaaaacccg gcccttgtcg ggaatgggat
      241 cgtcaaaaac cacatcaaag gcagagagcc tgtcggtagc cacgatcagc aagtaacgat
      301 ggagatcgta aatatcacgg actttccccc gccccactaa cgggaaactc ttcaattcgg
      361 tttcagttac cgtattcatg cctcatcccc catagctctt ttcatcactc gaattcatca
      421 ggattccttc cccagaacgc aagtaaagtg ggcttcacta gccagttcgt tgccgatata
      481 ggctttggcc tcgaacttcc cggtacgaat cttgaacttg ctcaggttaa cctcgatccg
      541 gagctgatcc ccggggcggg ccgacttccg gaacctggca tattcgatac cgccgaaaag
      601 cggcaccatt tctttctgat ccagcagatc aatgaggacg aatccggcca cttgggccat
      661 agcctctacc tgaagcgcgc ccggatagat cccgttttcg ctgagctccg cttgactaaa
      721 aaaatcatcc gcagtcacat tcttgattcc caccgctcgt ttaccttttt cgagttcgat
      781 aatccgatcc accagcagga aggggaagcg gtgcggtatg gcggccttaa tctgattgat
      841 atcgagcatt ccaatcacct catcagtgtc taaaatggcg gattccggtg ataaccatgg
      901 cgatattata gcgatcggcc atatcaatcg agtcctgatc gtggagtgag cctcccggat
      961 ggatgatggc agtgatacct gcctgtgccg cgacctccac agtgtcgggc atggggaagt
     1021 aggcatccga agccagacag gacccttttg ccttcgatcc cgcttgttcg atggcgatcc
     1081 gggccgaacc aacccggttc atctggcctg ctcccacccc gatggtaccg tgattccggg
     1141 tcaagacgat ggcgttggac ttgacgtacc gaaccacttt ccagccgaag aggagctcgt
     1201 ccaactccgc cggagtcggc tggcgtttgg tgactgtctt tagatccgcc gggttcagcg
     1261 taatgacgtc tgtttcctgg accagtaatc ctccggccac tttcttcata tccaaatcag
     1321 gagtgagaat agaacctgaa ttgacggcca gttccggtac ttccagcagg cgcaggtttt
     1381 tcttcttagc caaaagatca aaagccgcac cggtaaagga tggcgcgatg atgatctcta
     1441 agaacatctc actcatggga gtggcggtat ccaggtcaac ctcgcggttt aaagcgatga
     1501 tgccgccgaa gatcgacacc ggatcgcttt caaaagcctt ttgataagcc tcgaagatag
     1561 tagcccccgt tcccaccccg cacggattgg catgcttcac cgcaaccacg gccggttccc
     1621 cgaactcttt caccagttcc agggcggcat tgccgtcacc gatattgtta taagaaagct
     1681 ccttcccgtg aagctggcgg gcagtagtga tcgtccccgg tctgggagaa gtctcgcggt
     1741 aaaaggcggc tttctgatgg gggttctccc cgtaacgcag gctttgctgc ttgacaaagg
     1801 agagctgcaa cagatcgggg tactctttat ccgcctgatt gtacgcggtg ttaaagtaat
     1861 taaagatcat tgaatcataa agagcggtca tccgaaagac gtttacagcg cagcgttttc
     1921 gaaactccaa tgaagttccg ccctggttag cctcgatttc ctggatcaaa agcggataat
     1981 cggccgggtc gaccacaaca gtgacatcgc ggtggttttt ggcggcggag cgcagcatgg
     2041 acggaccgcc gatatcgata ttctcgatcg cttcctccag agtgaccccg gccttggcga
     2101 tggtagccgc aaagggataa aggttcacca cgaccaagtc gatgggttga atgcgctcat
     2161 gagccagctc ttgctggtgc tttgccgagt cgcgaattgc aaggagcgcg ccgtgaatcg
     2221 cgggatgtag agtcttaacc cgtccgtcta aaatctcagg gaatccggtt acatcggaga
     2281 tatacgtcac cgataggccg gcttcctgca acgccttata ggttccaccg gtggaaatga
     2341 gttccacgcc gtggctggtt aaaaatttgg ccagatccac cagcccggtt ttgtccgaga
     2401 cgctcaacaa cgccctttga attttagcca tctggtctcc tccttcaatt gaattgtgaa
     2461 gtttgaagtg tgaagttcaa gttttccgcc gcacccgcaa tccgtccacg atcacctgat
     2521 cccggacgat cagccggatc gcctccggat agatccggtg ctcaatctga agtaccttgg
     2581 ctgccagcgt ctctggtgta tcgtcatccg ccacagccac tacatcctgg gtaacgatgg
     2641 gcccgacatc ggtctccgcc gtcaccagat gaacggtggc cccggagaac ctggcccccg
     2701 aatccagtac cgctttatgc acatgcaatc cgtacatacc ccggccgcca aaggccggta
     2761 acaacgccgg atggatattc aggatccggc cgggatatgc ctcgatgagc agagggtcca
     2821 agatccgcat gaaaccggca agacagatca gatcgatccg ggattcacgc atcgcatcga
     2881 ggattgcccg ggtaaaagcg gtaaccgaag aaaactgatg gtgatcaatg acccgggtag
     2941 taattccatg ccgggtcgcc cgctcaagcg cataggcatc cggccggttg ctgatcacca
     3001 ctccgatctc cgccccgatt gttccggcta cggccgcgtc gatgattgat tgtaaattgg
     3061 ttccgccccc cgaaaccagg actccaatcc gcttaatatc caaaggaacc tccgtcagat
     3121 ccccggtacc gggtattgcg aacaaaatgc ctcaacttca ccgcgtaacc ccgccaaaac
     3181 cgcctcgtcc tgatgagccc ggagcgccct gacgatccat ccggcaatcc gccgcatctc
     3241 agcctctttc atcccccggg tggtcgcagc cggagtgcct aaccggatac cgctgggacg
     3301 gagcggcggg ttgggatcat ccgggatgat ctgtttgttg gtggtgattg cgatccggtc
     3361 caggacctgt tcggccacct tgccgtcgat tccgaaactg gttaccgtat gaatgaccat
     3421 catgtgatta tccgtgccgt tggtaatgag cttgaca
//
LOCUS       DASVMB010000494         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_722161,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000494 DASVMB010000000
VERSION     DASVMB010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722161"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..993)
                     /locus_tag="VF531_13830"
     CDS             complement(211..993)
                     /locus_tag="VF531_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715089.1"
                     /translation="MMDTQSRVVITNQPAAAIQPQTAPAVNLPLLTAPQEFMEVMKEN
                     RLDGENFRFDKIPMPSDGGITFVMMDEKGGTILRAELRGVILKFSHFKSWYQKSFDER
                     DENDDKRPDCFSQDGEIGSGCPDAGIPPEQKCEFCPKNQWGSDRRGGRGKDCADKIRI
                     YILLEESALPVFIDLPPASIGNFKEYRKRLTNKLKSLHGVVTVIGLEMDKSITGGITY
                     SKATFAKDADLNPDERAIIKQYIDTISPSLKQRARHHHHRLV"
     gene            1580..1939
                     /locus_tag="VF531_13835"
     CDS             1580..1939
                     /locus_tag="VF531_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7715090.1"
                     /translation="MAENYKFGLYLKNLRTQNNLTLKDLDRASGVSFGSISRMEDAKQ
                     DPNPDMLRKLAPALGVPYEEMLVMAGILDPKEETNKETNFPEDIQEIIRIANKLNHEQ
                     RQKLISIAKTVFPEVLN"
     gene            complement(2058..>3161)
                     /gene="groEL"
                     /locus_tag="VF531_13840"
     CDS             complement(2058..>3161)
                     /gene="groEL"
                     /locus_tag="VF531_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7715091.1"
                     /translation="EESKTMGTNLEVVEGMQFDRGYVSAYMVTDNERMEAVFDEPYIL
                     MTDKKITLIKDLLPILEKVIQRGRPLVLIAEDIEGEALATLILNKLRGTLNVVAVKAP
                     GFGDRRDAMLSDIAIVTGGQVISEKVGSTLENGTLEMLGQARQIKVTKDETTIVDGKG
                     SAQEIKNRISQIKVQIEDTTSDYDREKLQERLAKLSGGVAVIQVGAATETEMKEKKHR
                     IEDALSATRAAVEEGVVPGGGVTLLQIAPVLDTLKETGDVATGVSIVRRTLSEPLRQI
                     ANNAGVEGSVIVEKVGTLPKGQGYNALTGQYVDMVKEGIVDPAKVTRSALQNAASIAA
                     LLLTTECLVAEIPEKEKAPAMPPGGAPGMGMDY"
BASE COUNT          762 a          847 c          718 g          834 t
ORIGIN      
        1 cgtgccagtg caatcttctc aactttgctt tcgccttcgc cgccgcccgg cgccaatgat
       61 aaaagatggg tactttttgg agtcaataac aaaaactttt cccgaattcg cgggtaggaa
      121 cgatacatct cctcaatctc gtcaaatgtc ttccgcttca ttgcaagcct ccttatcccg
      181 ggtccgacgc ttcattcatt accaacttcc tcacacgagt cgatgatgat ggtgtcgcgc
      241 tctctgcttt agtgatgggg aaatagtgtc gatatactgt ttgatgatag cccgctcatc
      301 cggattcaga tcggcatctt tggcaaaggt tgccttggaa taagtaatcc cgccggtaat
      361 actcttgtcc atctccagac caatcacggt tactacgccg tgaagagatt tcagcttgtt
      421 tgtgagccgt tttcgatatt ccttgaaatt accgatactg gcggggggca ggtcgataaa
      481 taccggcagg gcgctttcct caagcaaaat ataaatccgt atcttgtcgg cgcagtcttt
      541 cccccggcca ccgcggcgat cgctgcccca ctgatttttc gggcaaaatt cacacttttg
      601 ttctgggggg atcccggcat ccgggcaacc cgatccgatt tcgccatcct gcgagaaaca
      661 atccgggcgc ttgtcgtcat tctcatcccg ttcatcaaag gatttttgat accaggattt
      721 gaagtgagag aacttaagga ttacaccccg cagctcggcg cgaaggattg tcccgccctt
      781 ctcatccatc atcacgaatg tgattccacc gtcgctgggc attgggattt tgtcaaatct
      841 gaagttctca ccgtcgagac ggttttcttt cataacctcc atgaattctt gcggagccgt
      901 caagagaggc agattaaccg ccggtgcagt ttgcggctga attgccgccg caggttgatt
      961 tgtgattact actcgtgact gagtatccat catctaggcc tcctaaaaat tttctagtat
     1021 atagcaatcg ttttaaggtt gctccttatc attcctcctt gacccactcc gcggcatatt
     1081 gcctgagagc tgtttgaata atcacctttt ctttaccatt gtgaagcgtt tgattatcta
     1141 gcccgctggc acatccctct ccaaactgca tttacaactt tgtaaattca agggtaaaaa
     1201 aagggtgctc ggcttaatac cggggaacgc ggaacgtaac tttttccggg acgattcccc
     1261 gacggccgaa aacctggggt ggtcaggcgg taaggacata taccataatt gcattctgct
     1321 aatcccggtt atttccgata attcaccgat tccccaacca ttctcgatac agatacggtt
     1381 gatctcatcc atattaagtt gatatcgcag cttaacaccc ataacgcatc ttcctttttt
     1441 atgtaatatc atttacaagt ttgtaaatac atcataccat cacgccgaaa gaaatgcaat
     1501 ccaaattttc aaagtcgtaa aaaattattt gcatacttgt aaatccggta ttataataga
     1561 cctgaaaggg atgattgccg tggcggaaaa ctataaattc gggctctacc taaaaaactt
     1621 acgcactcaa aataacttga ccttaaaaga tttggataga gcctccgggg ttagctttgg
     1681 atctatttcg aggatggaag atgccaagca ggatccgaat cccgacatgc ttcgcaaatt
     1741 agctcccgcc ctaggcgttc catatgaaga gatgttagtg atggcgggaa ttctcgatcc
     1801 gaaagaagaa acaaacaagg agacgaattt tccggaagac attcaggaaa tcatccgtat
     1861 tgccaataaa ttaaatcacg aacaacggca gaaattaatc tccattgcca aaacagtctt
     1921 tcctgaagtg ctcaattaaa agaggtggta atgaacgcct cggtttataa aaaggactgc
     1981 ccccagattc gggcagtcct ttttttgaaa ttgtgacgcc aatccggaca acccaagggt
     2041 tttccggaaa aggtatctta ataatccatc cccatgccgg gtgcgccgcc gggaggcatg
     2101 gccggagcct tctccttctc ggggatctcc gccaccaggc attcggtggt taaaagtaaa
     2161 gcggcgatac tggcggcatt ttgaagcgcg cttcgggtaa ccttggcggg gtcaacgatg
     2221 ccttccttca ccatatcgac atactgcccg gttaaggcgt tatagccctg acccttgggc
     2281 aaggtgccga ctttctccac aataaccgaa ccctcaactc cggcgttatt ggcaatctgc
     2341 cgcaacggct cggataaggt cctgcggacg atggaaacgc cggtagccac atcaccggtc
     2401 tccttgagcg tatcaagaac cggggcgatc tgtaagagtg tcacgccacc gccgggaacc
     2461 acgccttcct caaccgcagc gcgggtagcg gagagcgcat cctcgatccg gtgcttcttc
     2521 tctttcatct cggtctcggt cgcggcgccg acttggatga ccgctacacc gccggacaac
     2581 ttggccaagc gttcctgcag cttctcgcga tcgtaatccg aagtggtatc ctcgatctgc
     2641 actttgatct ggctgatccg gttcttaatc tcctgggcgc tgcctttacc gtcaacgatg
     2701 gtggtctcgt ccttcgtcac ctttatctga cgggcttggc ccagcatctc cagggttccg
     2761 ttctccaggg tactgccgac tttctcggaa ataacctggc caccggtgac gatggcgata
     2821 tcactgagca tcgcatcacg gcgatctccg aaaccgggag ccttcaccgc gactacattc
     2881 aaggtgccgc gaagcttatt caaaattaat gtggccaatg cctcgccctc aatatcctcg
     2941 gcgataagca ctaacggtct gccgcgttgg attactttct ccaggatcgg caataaatcc
     3001 ttgatcaggg taatcttttt atcggtcatt aagatatagg gttcatcaaa aacggcttcc
     3061 atccgctcat tgtcggtgac catgtaggcc gatacgtacc cgcggtcaaa ctgcatacct
     3121 tccacgactt ccaggttggt acccatcgtc ttggattcct c
//
LOCUS       DASVMB010000495         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_284923,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000495 DASVMB010000000
VERSION     DASVMB010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284923"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..587)
                     /locus_tag="VF531_13845"
     CDS             complement(15..587)
                     /locus_tag="VF531_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715092.1"
                     /translation="MGGLTFLRKINEAIDRRYLRKVGATPIPGSGQQLLLFCYHPYHA
                     RKELRLSETVIIRPGDTVSELHFSNTRIVEIGAQAGDRPLEWRIIDILKEEFSILAEA
                     CRNGTVPPEVKAFYGVNVMPAGAKRLGFTLVPLPRGFNRWWLGLWESLLRLVYYSYQT
                     KKKASLKRTMNDPYEIWISRDELIRRYARV"
     gene            complement(705..1478)
                     /locus_tag="VF531_13850"
     CDS             complement(705..1478)
                     /locus_tag="VF531_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7715093.1"
                     /translation="MGYFIIAVGLVLLWLLIYPLTDLFVRFVDPKVFKGGDPAARKLC
                     LTFDDGPDPRYTPELLQILRKEQVRATFFLVADKAVAAPDLVRQIIASGHEIGFHTFK
                     HRHAYLLFFNGSLKAVGEGRRKLEEVTGRPVFWFRPPWGALNLFQYLTARVLRLRLVL
                     WSANAQDWEIRTGTDGILERLKERVKPGGVIVLHDSGGDLGAPANTLAAVPEVIRWFK
                     GQGYQFVSLDEMATHPLAPVSPKDTPLPASGKEGEQDSK"
     gene            complement(1482..>2684)
                     /locus_tag="VF531_13855"
     CDS             complement(1482..>2684)
                     /locus_tag="VF531_13855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715094.1"
                     /translation="LFITFLPSFLKSLHYSTAVVGFVTFFNLIADNLTKGAAGWLIDR
                     YGPWPILLTGSMMVLTGVLIIIYFHQSIPILILAAILIGLGVAPTWPGAIGGSIQTVG
                     ESKRATIISIISVVWLGGGGLGPVLMGFLIDNRMRQTMLKLNLPLISAYRTGFLILFA
                     IAALSVIICILGFIGWHRVPHLRKLTEERQQIPIRHRLWAVFQRLWKVKGLVPGMFFQ
                     AFAMGLLLPNLLPYATTQLGLSEAQYSLLLLIGGAGAVLFMIPVGHFADHMGSRRFLV
                     SGFTLAALALFILVTYGNRTNIWWLVGFVGLSYALIQPAWNALLAGAVPPEQRGVLMG
                     LFMSVEGLGFAVGPAVGGLLGTIEKGAPGLLGRIGPATPFYVSGIFLLVMALVYLVHP
                     FHHYRIEE"
BASE COUNT          703 a          794 c          638 g          549 t
ORIGIN      
        1 aactataaaa cagctcaaac ccttgcgtag cggcggatta gctcgtctcg gctgatccag
       61 atctcgtatg ggtcattcat ggtccgtttc agcgaagcct tctttttggt ttgatacgaa
      121 taatacacca gccgcagcaa cgattcccac aaccccaacc accagcggtt aaaaccgcgg
      181 ggtagaggca caagggtaaa ccccagccgt ttggcgccgg ccggcatcac atttacgcca
      241 taaaacgcct ttacttccgg cggcaccgtc ccattccggc acgcctccgc caatatggag
      301 aactcttcct taagtatgtc gatgatccgc cactctaggg gtctatctcc cgcttgagcg
      361 ccgatctcta cgattcgggt attggagaaa tgcaactcac tcaccgtatc gccggggcga
      421 atgatgaccg tctcggatag ccgcagttcc ttgcgggcat ggtaaggatg atagcaaaac
      481 agcagtagtt gttggcccga gccggggatc ggggtcgccc cgacctttcg cagatacctc
      541 cgatcgatcg cctcgttaat cttgcgtaaa aatgtcaacc cgcccatccc aagcctccgt
      601 aaataaaatt gtgaagtgtg aagttgatgc ctacgactca ctcttgctct ctctggcgac
      661 aagcgccaaa gagagtaacc acaatccttc gtggcttttt tttactactt ggaatcttgt
      721 tccccctctt tcccgcttgc cgggagaggc gtgtccttag gggacacggg ggcaaggggg
      781 tgagtcgcca tctcatcgag gctcacgaac tgatatccct gccctttgaa ccaacggatc
      841 acttcgggca ccgcagctag ggtattggcc ggtgctccca ggtccccgcc cgaatcatgc
      901 aatacaataa cccctccggg tttcaccctt tccttcagcc gttcgaggat cccatccgtc
      961 ccggttctta tctcccaatc ctgtgcattc gcgctccata gtaccaaccg gagtcttaat
     1021 acccgcgccg tcaagtactg aaaaagattc agcgctcccc acggcggcct gaaccaaaac
     1081 accggccttc cggtgacctc ctcaagttta cgccgccctt ccccgaccgc cttcaggctc
     1141 ccgttgaaaa aaagcaggta ggcatgacgg tgcttgaagg tatgaaaccc aatctcatgc
     1201 ccggaagcaa tgatctgccg taccagatcc ggagcagcca cggctttatc agccactaaa
     1261 aaaaaagtcg cgcgaacctg ttctttacgg aggatttgca acagttccgg ggtgtagcgg
     1321 ggatccggac cgtcatcaaa ggtaagacag agtttacgcg ccgccgggtc gcccccctta
     1381 aaaacctttg ggtccacgaa tcggacgaac agatcagtaa ggggatatat caaaagccat
     1441 aacaaaacca agcccactgc gatgatgaaa taccccatga ctcattcctc gattcggtaa
     1501 tggtggaacg gatggaccag ataaaccagg gccattacga gcaaaaatat cccgctgaca
     1561 tagaacggcg tcgcaggtcc gattcgcccc aataagcccg gagctccctt ctcgatcgta
     1621 ccgagtaatc ctccgaccgc cggtccgacg gcaaaaccca atccctctac agacataaat
     1681 aagcccatca acactccacg ttgctcaggc gggactgccc cggccaacaa tgcgttccaa
     1741 gccggttgga tgagtgcgta ggaaaggccg acaaatccca ccaaccacca aatgttcgtc
     1801 cgattcccgt aggtgaccag gataaaaaga gcgagcgccg ccagagtaaa gccactgacc
     1861 aaaaacctcc ggctccccat atgatcggca aagtgtccga caggaatcat gaacagtacc
     1921 gcaccggccc cgcctatcaa aagcaacagg ctgtactggg cctccgataa acctaattga
     1981 gtcgtggcgt agggcaaaag attgggtaaa agtagaccca tggcaaatgc ctgaaagaac
     2041 attccgggaa caagcccttt aaccttccac aatcgttgga aaaccgccca taggcggtga
     2101 cgtatgggta tctgttgcct ttcttcggtg agtttccgga gatgcggtac ccggtgccac
     2161 ccgatgaatc ccagaataca aatgatcaca cttaacgctg cgatggcgaa gagaatcagg
     2221 aacccggtac gatatgcgct gattaacggc aggttcaact tgagcatggt ttgacgcatc
     2281 cgattgtcta tcagaaaccc cattaaaacc gggcccaaac ctccgcctcc caaccagacc
     2341 accgagatga tactgatgat cgtagcccgt ttggactcgc ctacggtctg aatcgatccc
     2401 ccaatcgccc ccggccatgt cggagcaact cccaggccaa tcaggattgc cgccaagatt
     2461 aagatcggaa tgctctgatg aaagtaaatg ataatcaaaa ctccggtcag aaccatcata
     2521 ctgccggtaa gcagaattgg ccacgggccg taacgatcga tgagccagcc ggctgcgccc
     2581 ttggtcaaat tatccgctat cagattaaag aaggtcacaa agcccactac agcggtagaa
     2641 taatgaagac tctttaaaaa actgggcaaa aaagttatga acag
//
LOCUS       DASVMB010000496         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_207314,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000496 DASVMB010000000
VERSION     DASVMB010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207314"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VF531_13860"
     CDS             <1..443
                     /locus_tag="VF531_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7715095.1"
                     /translation="IRDTLSEMQPQLGGVQVNAAMVPPEPVVLMDDYYFSQALQNIIM
                     NAIEAMERIPVKNRVINISVSIKNQWVELSIQDYGIGIPETDLDKIFQPFFSSKPITR
                     NWGMGLSLCHNIMNAHGGRISVESKVNEGTIFHLVLPLIRICSN"
     gene            559..1266
                     /locus_tag="VF531_13865"
     CDS             559..1266
                     /locus_tag="VF531_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715096.1"
                     /translation="MSGTIKVLIVEDDDLITKHYRTLLSKDPDLDMVGNANNGYEAVM
                     LAALQKPDVILLDIELEDKQAGLRAASEILSHMPEIKIVVLTVCETDETVFRAFELGV
                     TDYLLKNMPPEDVIKAVKDAYYDRSPIHANIAGKIKREFRRIKSSEDNLFHYFYIITQ
                     LTPAELENLELLLNGYSRDEICSKRHVESSTLKTQIYSTLKKFNKDNIKDVIQILRDL
                     HLLEILSYKKQAGLSRE"
     gene            complement(1401..2684)
                     /locus_tag="VF531_13870"
     CDS             complement(1401..2684)
                     /locus_tag="VF531_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEX7715097.1"
                     /translation="MNEKVALTPPMGWNSWDCYGASVTEAEVKGNAEYMAEYLKPFGW
                     EYIVVDIQWYEPQAKTSAYNPFVPLELDEYSRLVPAVNRFPSADHGKGFKPLADYLHG
                     LGLKFGIHILRGIPRQAVHANTAILGTEIRARAIAHPNSICPWNTDMYGVDPARPGAQ
                     AYYNSLFQLYASWNVDYVKVDDIAAPYNCGEIELISRAIANSGREMVLSLSPGPAPLE
                     QAERLMEHANMWRMTGDFWDRWEDLSAEFERCRQWSSYAGPGHWPDADMLPLGHIGIR
                     SCEHGVGDRWTRFSKNEQLTMVTLWCIFRSPLMFGGELRDNDDWTLSLLTNAEVLRVL
                     NHSHSGRQLFRNGLWDQHIAWTARDEDGSYYLAVFNTGTLETRVEIMLDRMNLSGPCR
                     VRDLWKRQDLEDVTWALLITIPSHGARLFKLNPIL"
     gene            complement(2822..4267)
                     /locus_tag="VF531_13875"
     CDS             complement(2822..4267)
                     /locus_tag="VF531_13875"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX7715098.1"
                     /translation="MSRPKRSRNLLVLLAVLVLLVGSYFFLKYRPVKSTADNSKTVEP
                     AVQDIVLTKLDEAKLSLIRIKSTRGSLSLSHKANGWVADSAPGLKLDQDVIDSLASTV
                     SNLYAQQEIDATPADLSQYGLDNPPITVTVLASDQKQAVIRLGNATPTGSSYYVMIQG
                     DPKVYTVSSYTGQSFDVALADLRDKSLPVINAQELKYLLIAPAGSRAIEIVPNNNAQN
                     TQFGVLNPWSMTKPYPEAKNVGNDQLQKILEAFASLTAISGFITDRPGDLGGYGLAKP
                     RLELIAKDKQNTLHLYVGKDSNESEVYCKLAGSPTVFTLSKDSLEAFNVKPFDLIEKF
                     ACILNIDDVDRVEIRAADKTHSILLTRKTIKTAKKDQPAEIATTYRMDGKKIPENVFK
                     KFYQSLIGLLMESESDRMPQDKNPEVTTTFHLNKGSQTERVVQVDYIPYNSDFYAVFR
                     NGKPLFLISRSQVQQMLTTAQAAIQGKLKAD"
     gene            complement(4264..>5260)
                     /locus_tag="VF531_13880"
     CDS             complement(4264..>5260)
                     /locus_tag="VF531_13880"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX7715099.1"
                     /translation="LVNYDYSNPYQEQVSSLAVEQRVTGAIQYVHLQKSPRIYLLKGH
                     GEADLPQEVAKQLDTDNYVTQDLNLLTKPIPQDADILMELAPSYDLTAAEVSKIREFL
                     ARNGRAIFLPTFQPKELPNFQSLLASYGVTLQPLIVVESDSSKYAGNPLFLLPEMADH
                     QILNPLAQNQMPLMIPQAQSLKMLDIKKRTTTIEPLLTTTASAWGKTDFNNTSLDRSP
                     NEPSGPFNLALAITDKENPEATTGAKIVVVANAAFLSSQFVNQVPGNINFLMNSLNWL
                     EDRGESLTIQPKSLMSFRLNMNAMQVLIYSALVVIVIPLIVLGTGVIVWLRRRHL"
BASE COUNT         1318 a         1196 c         1355 g         1391 t
ORIGIN      
        1 tcatccggga tacattaagt gaaatgcagc ctcagctagg cggagttcaa gtcaatgcgg
       61 ccatggttcc gccggaaccg gtcgttttaa tggatgatta ttatttttct caagcacttc
      121 aaaacattat tatgaatgcc atcgaagcga tggaaagaat tccggttaaa aaccgggtaa
      181 taaatatctc ggtatctatc aaaaaccagt gggtagagct ttccatacag gattacggca
      241 ttggtatccc tgaaaccgat ttggacaaga tatttcaacc atttttttca tctaaaccga
      301 ttactagaaa ctgggggatg ggtttgtcgc tttgccataa tattatgaat gcccatggag
      361 gaagaataag cgttgaaagc aaagtaaatg aaggtaccat ttttcatttg gtgcttccgt
      421 taatcaggat ttgcagcaac tgatgaaaaa tgtcgcgata aaagatattt tggggaaggt
      481 aattttgtga tagtaaagaa tttgttacag cggtatgtaa ataattagaa gaacgaatat
      541 tttgaaggcg gggtttgaat gtccggtacg attaaagtat tgattgtcga ggatgacgat
      601 ttaatcacca aacattaccg gactttgctt tcgaaagatc ctgatttgga catggtgggg
      661 aatgcgaata acgggtatga agctgttatg ctggcggcgc ttcagaaacc cgatgtaatt
      721 ttgcttgata ttgaattgga ggataagcaa gccgggttac gggcggcatc ggaaattctt
      781 tcccatatgc cggagattaa aattgtagtt ttgactgtat gcgagaccga cgagaccgta
      841 tttagagcct ttgaattagg ggtaaccgat tatttgttaa aaaatatgcc gccggaagat
      901 gttatcaaag ccgtaaaaga tgcttattat gatcgatccc ctatccatgc caatattgcc
      961 ggtaagatca aacgcgagtt tcgcagaata aaatcgtccg aagacaactt gtttcattac
     1021 ttttatatta tcactcagct tactccggcc gagttggaaa acctggaact attactgaat
     1081 ggctatagca gagatgaaat atgctccaaa aggcatgttg aatcttctac gttaaaaacg
     1141 caaatttata gcacactgaa aaagttcaat aaggataata tcaaggatgt gattcaaatt
     1201 ttacgcgatt tgcatctact ggagatatta tcctacaaga aacaagcggg cttaagccgc
     1261 gaataaaacg gtcaatccgg atccggactt cgtatatata atcctgaacc ggctttttct
     1321 gccggattct atacttttaa ggaagtcagc tactggtatg atttacggcc agggactatg
     1381 tcccggccgt attttttggt tcataatatt ggattaagct taaataatcg cgccccatga
     1441 gagggaatgg taatgaggag cgcccaggtt acatcttcca ggtcttgacg tttccataag
     1501 tctctgacac ggcaagggcc tgataaattc atccggtcaa gcatgatctc gacccgggtt
     1561 tccaaggtcc cggtattgaa aaccgccaaa taatagctgc cgtcttcatc ccgagcagtc
     1621 caggctatat gctggtccca gaggccgttg cggaataatt ggcggccgga gtgggagtgg
     1681 ttcagtaccc gcaatacttc ggcattggtc agcaaggaga gagtccaatc atcgttatcc
     1741 cgcaattcac cgccaaacat gagcggcgag cggaagatgc accaaagagt gaccatcgta
     1801 agttgctcgt ttttactaaa acgtgtccag cgatccccca ccccatgttc acaggagcga
     1861 attcctatat ggccaagagg taacatatct gcatctggcc agtgaccggg acccgcataa
     1921 ctactccact ggcggcacct ttcaaattca gcggacaagt cctcccatcg atcccagaaa
     1981 tcccccgtca tgcgccacat atttgcatgc tccattaaac gttccgcttg ctccaaggga
     2041 gccgggcccg gagagaggct caataccatc tcccgcccgg aattggcaat agcccggcta
     2101 atcagttcga tctctccgca attgtaagga gcggcgatat catcaacttt gacatagtcc
     2161 acattccaag aggcgtaaag ttgaaataga gaattgtaat aagcttgggc acccggacgg
     2221 gccggatcca cgccgtacat gtcggtattc cagggacaga tggaattggg atgcgcaatg
     2281 gcccgagccc taatctccgt acccagtatg gcggtgttgg catgaaccgc ctggcgagga
     2341 atgccgcgca agatatggat gccgaatttt aatccgagac catgcagata atcagctaaa
     2401 ggcttaaatc cttttccatg atccgcagag gggaagcggt tcactgcagg taccagccgt
     2461 gaatattcat ccagctccag cggaacaaag gggttgtatg ccgatgtctt ggcttggggt
     2521 tcataccatt gaatatcgac tacaatatac tcccagccga aaggttttaa atattcagcc
     2581 atatactcgg catttccctt cacttcagcc tcagttacgc tggctccgta acaatcccag
     2641 ctgttccagc ccatgggtgg cgttaacgcg actttctcgt tcatgaggga actccccttt
     2701 ttattcgttc actttcagct tattttcagc tgtttctgaa gtggcgggta atgggaaggg
     2761 aaacacacga aaacgaggcc caaaagggat gagttttcat gagattttgt gtgtttcgcg
     2821 gttagtccgc tttcaacttt ccctgaatag ccgcttgtgc agtagtcagc atctgctgca
     2881 cctggctgcg ggagatgagg aagagaggtt tcccgttgcg gaagaccgcg taaaagtcgg
     2941 aattgtacgg gatataatcc acctgcacca cccgctcggt ttgagaaccc ttgtttagat
     3001 gaaaggttgt agtgacttcg gggttcttat cctgcggcat ccggtcgctc tcggactcca
     3061 tcagcagccc gatgagggat tggtaaaact ttttaaagac attctcggga atctttttac
     3121 catccatccg gtaggtggtt gcaatctcgg cgggttggtc ttttttcgcc gttttgatcg
     3181 tttttcgcgt taagaggatg gaatgggttt tatccgcagc acgaatctcc acccggtcca
     3241 cgtcgtcgat attcaagatg caagcgaatt tctcaattag atcgaagggc ttgacattga
     3301 aagcctccag actatctttt gacaatgtga aaacggttgg agagccggcc agcttgcagt
     3361 agacctcgga ttcgttacta tcctttccga catataggtg gagcgtgttt tgtttatctt
     3421 tggctatcag ttcaagcctg ggtttggcca ggccataccc gcccaagtct ccgggccggt
     3481 ccgtaataaa gccggaaatg gcggtcaagg atgcgaaggc ttccagtatt ttttgaagtt
     3541 ggtcattccc gacgtttttc gcttccggat acggcttcgt cattgaccag ggatttaaga
     3601 ctccaaactg agtgttctgg gcattattgt tgggtacgat ctcaatggcc cggctcccgg
     3661 cgggagcgat caagagatac ttaagctcct gggcgttaat cacggggagg cttttatccc
     3721 gtagatcggc aagcgccaca tcgaaactct ggccggtgta actggaaacg gtataaacct
     3781 tgggatcacc ctggatcatc acgtagtatg aacttccggt gggagtggca ttgcctaatc
     3841 ggatgacggc ctgtttttga tcgcttgcca gaactgtcac tgtaatcggg gggttatcca
     3901 agccatactg tgacagatcg gcaggggtgg cgtcgatctc ctgctgggca tagagattgc
     3961 ttacagttga tgcgagtgaa tcaatgacat cttgatccag cttgagaccg ggagcggaat
     4021 cggctaccca tccgttggct ttatggctca gacttaagct gccacgggta gatttgatcc
     4081 ggatcaagct tagtttagcc tcgtccagct tggtgagaac gatgtcctga acggccggct
     4141 caacagtttt tgaattgtcg gcggtactct tgacaggccg gtattttaga aaaaagtagg
     4201 aaccgaccag caataccagg actgccagca aaacaaggag attccgggaa cgcttcggcc
     4261 ggctcataaa tgtctcctcc ttaaccagac gatgaccccg gttcccaaga cgatgagggg
     4321 aattacgatc actaccaggg ccgagtagat taggacctgc atcgcattca tatttaaacg
     4381 gaaagacatc aggctcttgg gttgaatggt caggctttca ccacgatctt cgagccaatt
     4441 caggctattc atcaggaagt tgatattacc gggcacctga ttgacaaatt gcgaactcag
     4501 gaaggccgca ttggccacga ccactatttt ggcgccggta gtggcctcgg gattctcctt
     4561 gtcggtgatg gccagggcca gattgaacgg gccggagggc tcgttcgggc ttctatcgag
     4621 cgaagtattg ttgaaatcgg tcttgcccca tgccgaggct gttgtggtca gaagcggctc
     4681 aatggttgta gtccgtttct tgatatccag catcttgagg ctctgggcct gcgggatcat
     4741 gagcggcatt tggttttggg ccaatgggtt tagaatctgg tgatccgcca tctccgggag
     4801 gaggaataac ggattgcccg cgtattttga actgtcggac tccacgacga tcaggggctg
     4861 caaggttacg ccatagcttg ccagcaagct ttgaaagttt ggtaattctt tgggttgaaa
     4921 ggtaggcaaa aagattgccc ggccgttacg ggccaggaac tccctgattt tgcttacttc
     4981 ggcggcggtc aggtcgtagc tcggcgccag ttccatcagg atatccgcat cttggggtat
     5041 gggtttcgtc aagaggttga gatcctgggt tacatagttg tcggtatcca gctgtttggc
     5101 cacttcctgc gggagatcgg cttcgccgtg gcctttcaaa agatagatcc tggggctctt
     5161 ttgcaggtgg acgtattgaa tcgccccggt gacccgctgc tctactgcca atgatgatac
     5221 ctgctcctgg taggggttgc tgtaatcgta gtttaccaga
//
LOCUS       DASVMB010000497         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_651151,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000497 DASVMB010000000
VERSION     DASVMB010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651151"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2498)
                     /locus_tag="VF531_13885"
     CDS             complement(<1..2498)
                     /locus_tag="VF531_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7715100.1"
                     /translation="MALSEEWRDRITDWLNELKREFYRKLGDIQLTGFTTCDHLSMEE
                     ALNRPCQPLSPGTQWGAKWEYGWFRGEITLPSAAAGERIVAMIETGTNPAATKNILVL
                     DTGSENLVYVNGQMSGATRSRWYRDLIAYPNQILISRDGVPGTNYQLFFECYAGHGAM
                     PTAVGPVPPGRDAVPEPPPQQVVIGESSFGIWEELLYQLWLDANTLWELRDKLDPNSL
                     RVAEIDSGLRDFATILDFEVPYPRMIATAKAARARLRPLLECINGSTAPTLYAFGHGH
                     IDLAWLWPLAETVRKGARTFTNQLELLKEYPEYRFLQSTPALYRMMQEHYPALYDRIK
                     EAVKNGGIIPEGGMWVEADTNITGGESLIRQFIYGKRFFSTEFGIESQMLWLPDVFGY
                     SGNLPQIMRGCGIKYFATGKLFWNYHGGAAFPYNTFMWKGIDGSEILTHLCDGYGAET
                     SPAPLIRTWETRVQKDGCSARLLPFGWGDGGGGPTRDHLEYLRREKNLEGMPQARIAS
                     PLEFFKNIEDQEPIAKYVGELYFQAHRGTYTSQARIKQANRRSELALREAELWSTVAA
                     RLNGFEYPRIDLETAWKKVLLNQFHDILPGSSIHRVYEEAETAYRQILDLAGRVTGWA
                     LAGFINHAEQDVEALTVFNSLSWERTVLVKLPWSGGAAKADGVILPGQVIDGATLVSL
                     TVPACGWTTLYPAACLAPAVPVQVGDRYLENEFLRVELDDRGALTRILDKEADRELIT
                     APANVFKMYQDIPTRNDAWDIDSFYERTPLELDEPAEIEVLQVGPLLGSIRVTRGLHQ
                     SQLVQEISLSRGSRAIEFNTRIDWRERHKLLKVN"
     gene            complement(2558..>3292)
                     /locus_tag="VF531_13890"
     CDS             complement(2558..>3292)
                     /locus_tag="VF531_13890"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX7715101.1"
                     /translation="EVGSGVAMNCWLPMPFARRARVEVVNEQADPVNSFYFYIDFQEH
                     GSLPDDILYLHTFWKRENPCNGWKGEGSVWGSPEWQRRHEGAEDHNKSDNNNYLILDA
                     EGYGHYIGMNMSIDHLYKGWWGEGDDMIFIDRDGERQWPPDLHGTGSEDYLCQAWGLQ
                     KVSHFYCGTSWCELDNYDSWGKVSVYRYHIQDPIPFTKNIRVSIEHGHANDRSDDYSS
                     VAYWYQTEPHKVFAPMLPVYKRLPNP"
BASE COUNT          737 a          941 c          817 g          797 t
ORIGIN      
        1 aagttcactt ttaacaactt atgccgctcc cgccagtcaa tccgagtatt aaactcgatg
       61 gcgcggctgc cccggctaag acttatctcc tggacaagtt gggattgatg gagcccgcgg
      121 gtcacccgga tgcttcccaa gagcggacca acctgcaata cctctatttc agccggttca
      181 tccaattcaa gcggggttcg ttcataaaag ctgtcgatat cccaggcgtc attccgggtg
      241 gggatgtcct gatacatttt aaatacattg gccggtgccg taatcagctc ccggtcggcc
      301 tctttatcaa ggatgcgggt gagcgcaccc cggtcatcca attccacccg cagaaactca
      361 ttctcaaggt atctatcgcc gacttggacg ggaacagccg gcgccaaaca agcggcggga
      421 tatagagttg tccatccgca cgccggtacg gtcaggctga ccaaagttgc accatcgatc
      481 acctgtcccg gaagtatgac cccgtcggcc ttggcggctc cgccggacca tggcagcttc
      541 accaaaaccg tccgttccca ggaaagtgag ttgaaaacgg taagcgcctc gacatcttgc
      601 tccgcatgat tgatgaatcc ggcaagggcc caacccgtta ctctgcccgc cagatcaagt
      661 atttgccggt atgcggtctc cgcctcctca taaacccggt ggattgagga accgggcagg
      721 atatcatgaa actggttgag taaaaccttt ttccaggcag tttcgagatc gatccggggg
      781 tactcaaaac cattcaaccg ggcggcaacg gtactccata attccgcttc ccgtaacgcc
      841 aattcgctgc ggcggttagc ctgcttgatc cgggcttggg acgtgtaagt gccgcgatgt
      901 gcctggaagt ataactcccc cacatacttg gcgatgggtt cctgatcctc gatattcttg
      961 aagaattcca acggtgaagc aatcctggcc tggggcatgc cctcaaggtt tttctcgcgc
     1021 cgcaagtatt caaggtgatc acgggttggc ccgccgccgc cgtcacccca gccaaagggg
     1081 agcagccggg cggaacaccc gtccttttga acccgggtct cccaggtccg tatgagtggc
     1141 gccgggctgg tttcggcgcc gtaaccgtcg cataggtgcg tcagaatttc ggaaccgtcg
     1201 attcctttcc acataaaggt gttatacgga aatgccgcac cgccgtggta gttccaaaag
     1261 agttttccag tggcgaagta tttgatgcca cagcctctca tgatttgagg caggtttccg
     1321 gaataaccga atacatcggg cagccaaagc atctgactct ctataccgaa ctcggtgctg
     1381 aaaaatcttt tcccgtaaat gaactgacgg attaagctct ctccgccggt gatgttggta
     1441 tcagcctcca cccacatacc gccctccggg atgatccccc cgttttttac ggcttcctta
     1501 atccggtcgt ataacgcggg gtaatgctct tgcatcatcc gataaagggc tggagtgctt
     1561 tgtaagaacc ggtactccgg atattccttg aggagctcca gttgattggt aaaggtacgt
     1621 gccccttttc gtactgtctc ggccagcggc cataaccagg ctagatcgat atgaccgtgt
     1681 ccaaaggcat aaagggtagg ggcggtcgat ccgttgatac actccaataa cggtctgagt
     1741 ctggcacggg ccgccttcgc cgtggcgatc atccggggat acggcacctc gaaatccagg
     1801 atagtcgcaa aatccctaag tccgctatcg atctccgcca cccgcaatga attgggatcc
     1861 agtttatccc ggagttccca tagggtattg gcatccaacc acaactggta aaggagctcc
     1921 tcccagatcc cgaagctgga ttcgccgatg acgacctgtt gcggcggagg ttcggggact
     1981 gcgtcccgtc caggaggcac agggcctacc gcggtaggca tcgctccatg accggcataa
     2041 cattcgaaaa acagctgata attagttccg ggtaccccat cccggctaat taatatttga
     2101 ttgggatacg cgataagatc ccggtaccac cgggaacggg tcgcgccact catttgaccg
     2161 tttacataga ccagattttc cgatccggta tccagcacca gaatattctt ggtcgcagca
     2221 ggattggtac cggtctcgat cattgccaca atgcgctctc cggcagccgc agacggtaac
     2281 gtaatctccc cgcgaaacca gccatattcc catttcgctc cccattgggt tcccggggac
     2341 aggggctggc agggccggtt caaagcctct tccatcgaga ggtggtcgca ggtagtgaac
     2401 cccgttaact gaatatctcc gagttttctg taaaactccc gtttcagctc gttaagccag
     2461 tctgtaatcc ggtcccgcca ttcttcagat aacgccatga tttatctccc tgatttctaa
     2521 actgttctgt cttcgggtag ttcctggtga atcgttctta tgggttgggc aatcttttat
     2581 atacgggaag catgggcgca aacactttgt gcggttcggt ctgataccag taagctacgg
     2641 agctgtaatc atcgcttcga tcgttggcgt gcccgtgctc aatacttacc ctgatgtttt
     2701 tggtaaacgg tatcgggtcc tgaatatgat accgataaac tgaaaccttt ccccagctat
     2761 cataattgtc cagctcacac caagatgttc cgcaatagaa atgtgatact ttttgtaatc
     2821 cccacgcttg gcaaagataa tcctcactgc ccgtgccatg cagatcaggg ggccattgcc
     2881 gctcgccgtc acgatcaatg aagatcatat catccccttc gccccaccag cctttgtaaa
     2941 gatgatcaat actcatattc atgccgatgt aatggccgta accttcggca tccaatatca
     3001 aataattatt gttgtcgctt ttgttgtgat cttcggcgcc ttcgtgcctt cgctgccatt
     3061 ccggtgaacc ccagacactt ccttcacctt tccaaccgtt gcagggattt tccctcttcc
     3121 aaaacgtgtg cagatacagt atgtcatccg gaagtgaacc gtgttcctgg aagtcgatat
     3181 agaagtaaaa ggagttgacg ggatctgcct gctcattaac aacttcgacc cgcgcccgcc
     3241 gggcaaatgg catgggcagc caacagttca tcgccactcc gcttccgacc tc
//
LOCUS       DASVMB010000498         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_137093,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000498 DASVMB010000000
VERSION     DASVMB010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137093"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VF531_13895"
     CDS             complement(<1..1529)
                     /locus_tag="VF531_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7715102.1"
                     /translation="MLEKFLPGEKFESYEDFKENYRVNLPLNFNFAYDVVDAWALEKP
                     EKRALVWCDDHGGERIFSFADLKRLSDQAANLFQSRGIAKGDTVLLILKRRWQYWVCT
                     LGLQKIGAVSIPATVQLTKKDIIYRNNNASVKMIIAVDEVAEHVEAALPESPTVICMA
                     TVDEPRKGWLDFNTALLQASPSWSRPLGQNQAGGADPMLMYFTSGTTGMPKIVLHDFY
                     YPLGHIVTAHYWQRLGEEGLHLTVSESGWAKCGWGKIYGQWICGVAIFVYDMERFEPA
                     KLMAMIEHYRITSFCAPPTIYRFLIKEDLSKHDFSSIRVACTAGEPLNPEVYNQFKKA
                     TGLDIIEGFGQSETTVLCANFEWLTPKPGSMGKPSPLYDIDIQDEQGRSCPPGVEGNI
                     VIRNLDRGLPPGLFKGYFGDEAATRKVWHDGIYDTGDTAWRDEDGYFWFVGRSDDVIK
                     CSGYRIGPFEVESALIEHPAVLECAITAAPDPIRGQVVKATVVLAKGRQPGPELVKEL
                     Q"
     gene            complement(1618..>1843)
                     /locus_tag="VF531_13900"
     CDS             complement(1618..>1843)
                     /locus_tag="VF531_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7715103.1"
                     /translation="LVTIEPEENKQMSLVTHPGQEFNYVLQGTIKVILGGTAITLSKG
                     DSIYFDPAIPHGQMAVDGKSAQFLTVILHD"
BASE COUNT          432 a          488 c          475 g          448 t
ORIGIN      
        1 tcctgaagtt cttttacaag ctcagggccg ggttgtcggc ctttggcaag caccaccgtg
       61 gctttgacca cttggccccg tatcggatcg ggagccgccg tgattgcgca ttcgagcact
      121 gccgggtgtt cgatgagggc gctttccacc tcgaagggtc cgatgcgata tcccgagcat
      181 ttaatgacat catcggaacg gcccacaaac cagaaatagc cgtcctcatc ccgccaggcc
      241 gtatcgccgg tatcataaat tccatcatgc cataccttgc gggtggccgc ctcatctccg
      301 aagtagcctt tgaataaccc cggcggtaga ccgcggtcaa gattcctgat gacgatattg
      361 ccctcgactc ccggagggca agaccgcccc tgctcgtcct ggatatcaat atcgtaaagc
      421 ggagacggtt tacccatgga tcccggtttg ggagtgagcc attcaaagtt ggcgcagagt
      481 acggtggtct ccgactgacc gaatccttcg ataatatcaa gtccggttgc ttttttaaat
      541 tggttgtaaa cctcgggatt gagaggctca ccggcggtgc aggccacccg gatgctggag
      601 aagtcatgct ttgaaagatc ttctttgatt agaaaccggt aaatcgtcgg cggagcgcaa
      661 aatgaggtga tgcgatagtg ttcgatcatg gccattaatt tcgcgggctc gaatcgttcc
      721 atatcataga cgaagatcgc caccccacag atccattggc cataaatctt cccccagccg
      781 cattttgccc aacccgactc cgaaacagtc aggtgcaaac cctcttcacc cagccgctgc
      841 cagtaatggg cggtcacgat atgtcccagg gggtagtaaa aatcatgaag cacgatcttg
      901 ggcatacccg tggttccgga ggtaaaatac atcaacatcg gatctgcacc gccggcctgg
      961 ttctgtccca gggggcggga ccaggatggg gatgcctgaa ggagcgcggt gttaaaatca
     1021 agccatcctt tacggggctc atccaccgtg gccatgcaaa tcactgtcgg cgattccggg
     1081 agtgcggcct ctacatgttc cgccacctca tcaacggcga tgatcatctt gactgaagcg
     1141 ttgttgtttc gataaataat atctttcttc gtaagttgca ccgtggccgg aatggaaacg
     1201 gccccgattt tttgcaggcc caaggtgcat acccagtatt gccagcgccg tttgaggata
     1261 agcaagacag tatcaccttt ggcgataccc cgggattgaa aaaggttggc cgcctgatca
     1321 ctcaggcgtt tcagatcggc gaaactgaag atccgttccc cgccgtgatc gtcacaccag
     1381 accaaggccc ttttttccgg tttttccagg gcccaggcat cgacaacatc ataagcaaag
     1441 ttgaaattca agggaagatt aacccggtaa ttttccttaa aatcctcata ggattcgaat
     1501 ttttctccgg gtaagaactt ttccagcatt ggataatgct cctttgtaac gaagttaaat
     1561 tatcatgtag gattaggtcg ggcgggattc ggcatgcagg tttagtcatg tgcaggttta
     1621 gtcatgcagg attaccgtga gaaattgggc ggactttcca tcgaccgcca tctggccgtg
     1681 aggaatggcc ggatcgaaat aaatggaatc acccttggac agagttattg ccgtcccgcc
     1741 cagaataacc ttgatagtgc cttgcagtac atagttgaac tcttgaccgg ggtgagtcac
     1801 cagactcatc tgtttattct cctccggttc gattgtcacc aat
//
LOCUS       DASVMB010000499         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_358381,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000499 DASVMB010000000
VERSION     DASVMB010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358381"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF531_13905"
     CDS             <1..261
                     /locus_tag="VF531_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715104.1"
                     /translation="YVGETPFFLNKYSGEVIRSLNGGKNFNTSGSNTMQIETSQIQWD
                     DGKGPADICYALLYLRDGRQFIGTANPTNFYFVSRSEMVEVK"
     gene            427..1209
                     /locus_tag="VF531_13910"
     CDS             427..1209
                     /locus_tag="VF531_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7715105.1"
                     /translation="MSMYEGFIYAQQITCPAGAVPYTIQAGDTIYRIAGRLGTTVAAI
                     LALNPGINPNALQIGQQICVPGAAPPPTCTGTTYTIRPGDTFYAIAARYGISLQALIA
                     ANPDVDPNRLVVGQTICIPTVTPPAPVTAPCSALLQPVTASLPPAAQIPTGAAVVRQV
                     AMSTREYTVVASALPEPGTLGNFNGYVAVLSLLAETGQRMTVNIRLGSSQFGTQTVAW
                     AGSTITAPPPVPGDTVEVRPANIATGRQGPALLRADLGTCRT"
     gene            1486..2223
                     /locus_tag="VF531_13915"
     CDS             1486..2223
                     /locus_tag="VF531_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7715106.1"
                     /translation="MIPEEYLIQATQVFCQGGTVYTISPGDSLFGLAQRFNTTVQAIL
                     TANPGIDPANLQLGQRICIPVTPTPGPCPGGFLYTIRAGETYYSIARRFGIAVPALAA
                     ANPGVDPSQLRIGQQICVPAPAPAPGPCPGGFLYSIRTGDTYFSLARRFNITLQALIS
                     ANPGVDPARLQIGQQICIPAPAPSPFPCPGGFLYTITAGDTFFSLARRFGTTGDAIIA
                     ANPGVDPERLLVGQQICIPVPAPPSIS"
     gene            2376..2696
                     /locus_tag="VF531_13920"
     CDS             2376..2696
                     /locus_tag="VF531_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HEX7715107.1"
                     /translation="MDNNNYNDPYGMGHVHAYSLAVHPQNGHSHLAMGLTGPALPIPG
                     GHFHQLAGYVSRKDGHIHSYSLSTSPAMQIAGASERHVHYFRGETSHNDGHHHEMEAY
                     LMPE"
     gene            complement(2892..3395)
                     /locus_tag="VF531_13925"
     CDS             complement(2892..3395)
                     /locus_tag="VF531_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7715108.1"
                     /translation="MQREKAVTEDDWQFRSFLKGCRDKVIDRAVSELYREAIQAIDCR
                     KCGQCCRELLPILTSADIEKFATGMGQEPERFTSEYLTWDPEAEGYVWRKKPCPFLKH
                     NQCSHYPFRPVDCAGYPHLDVPLVSSRLTMIIENLEICPRIAYVWKRLKEHEAFGDWR
                     REVEEYW"
     gene            complement(3601..4548)
                     /locus_tag="VF531_13930"
     CDS             complement(3601..4548)
                     /locus_tag="VF531_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715109.1"
                     /translation="MSQNPVEVYVSNYLEPRLNALTDNPDAVSLVVQDIKNRLAAIIT
                     RWNDEEFRRTLLVTGFEEASFYQPASDIETRALVVLAVRNSKLEDLGSTEQAARTLGL
                     SSPVIFDDDIPGFTQEAIAYFSQYQPGELAGEIPCESYDPYQMLSIRFPVAWQALSHL
                     GWWERVESTYDPVLEPASPELNFLPDQTWDQDIQPFVGEIQSGLNPAFTPGLLGILGQ
                     IQIGKLQLFYVDSFKSLTRHPEKLFKTLEWVLRAGVPVITANFYLENGYVARRQTLVR
                     PQHCKEDTFANLKNLDGLTAGHREMLIRVAKNTIDATGE"
     gene            complement(4591..5124)
                     /locus_tag="VF531_13935"
     CDS             complement(4591..5124)
                     /locus_tag="VF531_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715110.1"
                     /translation="MEKDNELVPKNSEIVQKQSKGRIEIIQTNNCTFERELPGEIEIR
                     QVNSSIVKPKKPKEQNEKRQVYDVNDDDSKVLVIHIKDSEQKEPPSPAILNPILNAME
                     ILAGGIRAHLGNQDLKKNLLDEVKNSRELLQSGRTNQAFLKLTIIGDEVQRVRQKPVD
                     IIHKDLIKTKRQLFDLV"
     gene            5342..5482
                     /locus_tag="VF531_13940"
     CDS             5342..5482
                     /locus_tag="VF531_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715111.1"
                     /translation="MGVPNEDVDAKARVSVFQSNSAKIFARVSAEEEERRRKGHGAAG
                     VE"
     gene            5731..5874
                     /locus_tag="VF531_13945"
     CDS             5731..5874
                     /locus_tag="VF531_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715112.1"
                     /translation="MPNIGEDPEAKARVTVNQRNSATVFVRASAEEEERRRRRKHQEY
                     EEE"
     gene            6208..>6565
                     /locus_tag="VF531_13950"
     CDS             6208..>6565
                     /locus_tag="VF531_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7715113.1"
                     /translation="MFNLTPFQFNDIERNLFPAFFGEEFLGNSLGSFRTDLHDNGKEY
                     LIEAELPGFAKDEIQVKVEDNSLTISAQRHEAKEEKRDNYIRRERCSGRLVRSFALDG
                     VQSEGIKAEFKDGILKL"
BASE COUNT         1679 a         1677 c         1486 g         1723 t
ORIGIN      
        1 tatgtcggtg aaaccccctt cttcctcaat aaatacagtg gtgaggttat tcggagccta
       61 aacggcggca agaacttcaa tacttccggt agcaatacca tgcagatcga gacctcacag
      121 attcaatggg atgacggtaa agggcccgcc gatatctgct acgccttgtt gtaccttcgg
      181 gacggaaggc aattcatcgg aaccgcgaat cccaccaact tttattttgt ctcccgttct
      241 gaaatggttg aagtcaaata agagctaaag agtcaatgtt aataaatatc ctccggcgga
      301 gagagccggg ggatatttat tattatgcca ttcatttcga attgcgctga tggaaaaaaa
      361 gcagacaggt ttcacagtcc ttattatctt ttcatatatt ataacagcac gaaaggatgt
      421 gaaacgatgt caatgtatga aggttttatc tatgcccaac aaataacctg tccggccgga
      481 gcggttccct ataccattca agccggcgat accatatacc ggattgccgg acgcctggga
      541 acaacggttg ccgccatact cgccttaaat cccggcatca atcccaacgc gcttcaaatc
      601 ggccaacaaa tatgtgttcc cggtgccgcg cctcctccga cttgcaccgg aaccacttat
      661 accattcggc ccggcgacac tttttatgcc atcgccgccc ggtacggcat ttccctccaa
      721 gccttgatag cggcaaatcc cgacgttgat cccaaccgac tggtagtcgg gcaaaccatc
      781 tgcatcccca ctgtgacccc gcctgctccg gtgaccgccc cgtgcagcgc cttattacag
      841 ccggttacgg cctcgttgcc tccggctgcg cagatcccta ccggtgctgc tgtcgtccgg
      901 caggtagcca tgagcacccg ggaatacaca gtagtcgctt cggctctccc tgaacccggt
      961 acacttggta atttcaacgg ctatgtggct gttctcagtc tccttgccga aaccggccaa
     1021 agaatgaccg tgaatatccg gctgggatca tcgcaattcg gaacccagac tgtagcctgg
     1081 gccggtagta ccatcaccgc gccgccgcct gttcccggcg ataccgttga ggttcgcccg
     1141 gcgaacattg ccaccggtag gcaaggtccg gctttactcc gcgccgatct cgggacctgc
     1201 cgcacctgag cccttattca actttctatg ccgtcctatg gtatttcaaa gccgccccaa
     1261 aaacgggcgg tattttttgg gctggtggaa tacaaattgc atacccatta agcaatcaac
     1321 tcagatgatt aataccttat tggccttagc ttcactgcaa tatgagaaag ttatgttatc
     1381 tctctcaggt ctaaaagaag attacttaca ttgtcccggt ccctattact ttggcagaac
     1441 atacattaaa aataaataac caaagaataa ggaggaatca gaataatgat tccggaagaa
     1501 tatttaatcc aggcaaccca ggttttttgc cagggcggga cagtctatac aatcagcccg
     1561 ggagactccc tctttggcct ggcccaaaga tttaatacta cggtccaggc cattttgacg
     1621 gctaatccgg gtatcgatcc tgctaattta caattaggtc agagaatctg tatacccgtg
     1681 acaccaactc ccggaccctg tccaggcggc tttctttata cgattagagc gggggaaact
     1741 tactatagca ttgcccggcg tttcggtatt gcggtccctg ccttagctgc cgctaatcca
     1801 ggtgtggacc caagccaact ccggattggc cagcaaatct gcgttcccgc tccggctcct
     1861 gcgcccggtc cgtgccccgg aggatttctg tatagcatca gaacgggaga cacttacttc
     1921 agtctggcaa gacgcttcaa tatcaccctt caagccttga tctcagccaa ccccggtgta
     1981 gatcccgcca ggctgcagat tggtcagcag atctgtatcc cggctcctgc tccttcacct
     2041 ttcccatgcc cgggaggatt cctttatacg atcacggcag gagacacttt tttcagtctg
     2101 gcccgacgtt ttggtaccac tggcgatgcc ataatcgccg ccaaccccgg cgtagatccg
     2161 gagagattac tcgtgggcca gcagatctgt atcccggttc cagctccacc gtcaatttca
     2221 tgacttgcgg ggataccttc ttcagccggt gttcgtgtca aggctttagc ggcaaatgaa
     2281 caatccgttt tccatttgga aaaatagtct cttatcccgg gacaatcgac tgatgaggga
     2341 atacaatgtg gctgtcatca aaggagggat tatttatgga taacaataac tataatgacc
     2401 catacggcat ggggcatgtt catgcgtaca gtctggcggt tcatccccaa aacggccatt
     2461 cccatctggc gatggggttg acaggtccgg ccctacccat ccccggcgga catttccacc
     2521 aactcgcggg gtatgtctct cgtaaagacg gtcatatcca cagttattcg ctatcgacca
     2581 gtccggctat gcagattgcg ggtgcaagtg aaaggcatgt tcattatttt aggggcgaaa
     2641 cttcacataa cgacggacat catcatgaga tggaagccta tctgatgccg gaataagcat
     2701 gccgggatct taatctccct actcataacc ttatccactc ctgtaaaata aagaaccccg
     2761 gcctcaaggt cgtgggtcct ttcaaaaaaa gcatagataa tatattttaa aggagtaccc
     2821 aattttaatt gttatctgct ggcaatacct ttaatcctga aaatccggtc tactcccggt
     2881 caatcctggt cctaccagta ctcctcaact tcccggcgcc aatccccaaa ggcctcatgt
     2941 tccttgagcc ttttccagac atatgcgatg cgcgggcaga tctccaggtt ctcaatgatc
     3001 atggtcaggc ggcttgagac caagggcaca tcaaggtgcg gatatccggc acaatccacc
     3061 ggccggaatg gatagtggct acactgatta tgttttaaaa acggacacgg tttctttctc
     3121 caaacatacc cttccgcctc cggatcccag gtcagatact ccgaagtgaa tcgttccggt
     3181 tcctgcccca tccctgttgc gaatttttcg atgtcggcgg aggtcaggat tgggagtaac
     3241 tcccggcaac actggccgca ttttcgacag tcgatggcct gaatcgcctc ccggtaaagc
     3301 tcagaaaccg cccggtctat caccttgtcc cgacagccct ttaaaaagga ccggaattgc
     3361 caatcgtctt cggtgaccgc tttctcccgt tgcatctaaa atcctcccag gttttaaaac
     3421 ttgtccgtat tctgtaccca gatcagggct tgtcattcta caattggaat aacgcttcat
     3481 ctaaaaaaaa tgagccgcaa acagagttat tacagtttcg gtcccaatac tccctgttat
     3541 ctaattgtac accaaagccc attcatcggg tatctcattt aaacatttcc ttggccgacg
     3601 ttattctccg gttgcatcaa tcgtgttttt agccacccgg atcaacatct cccgatgtcc
     3661 ggccgtcaac ccatccagat ttttcaggtt ggcaaaggtg tcttccttgc agtgctgagg
     3721 tcttaccaaa gtttggcggc gcgccacata cccgttctcc agataaaaat tggcggtgat
     3781 caccggtact ccggcccgca gcacccactc caaggtttta aagagtttct cggggtgccg
     3841 ggtaagggat ttaaatgaat ccacatagaa taactgtaac tttcctatct gaatctgtcc
     3901 taagatcccg agcagccctg gagtaaacgc gggattgagc ccgctttgga tctccccaac
     3961 aaagggttga atatcctgat cccaggtttg gtccggcaag aaattcagct caggggatgc
     4021 aggctctagc accgggtcat aggtggattc cacccgttcc caccagccca ggtgtgatag
     4081 ggcttgccat gcaactggga aacggattga cagcatttga taagggtcat aagattcaca
     4141 cgggatttca cccgctaatt ctccgggttg atactgtgaa aaataagcaa tggcttcttg
     4201 ggtaaaccca ggaatatcgt catcgaatat gacagggctt gataaaccca atgttcgggc
     4261 ggcctgttcg gtactaccca gatcttctaa cttgctattt ctgactgcca gcaccaccaa
     4321 agcccgggtc tcgatatccg aagccggttg ataaaatgat gcctcttcaa agccggtgac
     4381 cagcagggta cggcggaact cttcatcatt ccaacgtgtg attatagcag ccaagcgatt
     4441 cttgatatct tggaccacaa gggaaacagc atcgggattg tcggttaaag catttaaccg
     4501 gggttccaaa taattcgata cgtaaacctc caccggattt tgggacatat tcctcactcc
     4561 agactttttg gttctcggga tgccgatttc ctataccaaa tcgaagagct gacgttttgt
     4621 tttaatcaga tccttatgga taatgtcaac cggcttttgt cggacccgct gaacttcgtc
     4681 gccgatgatc gttaacttga ggaacgcctg atttgtcctt cccgattgta ataactcgcg
     4741 ggaattttta acctcatcga gcaggttctt tttcaaatcc tgattgccca gatgtgcccg
     4801 gataccgccg gctaatattt ccatggcgtt cagtatcgga ttaagaatcg ccggggaggg
     4861 cggttccttt tgctccgaat ccttgatatg gatgactaaa accttggaat cgtcgtcatt
     4921 cacatcatat acctgtcttt tctcattttg ttcttttggt tttttaggtt tgacaatgct
     4981 tgaattgact tgacggatct cgatctctcc gggaagttcc ctctcgaaag tgcaattatt
     5041 ggtctggatg atctcgatcc tgcctttaga ttgtttttgg acaatttccg aattttttgg
     5101 gaccaactcg ttatcctttt ccaattgacg attcctccca tttttttgat attggactgt
     5161 ttttaagtaa tgaggatgaa tcaataatca ttggtcaaat atattagatt tatgggattt
     5221 attccaaaat gtaataaatg atgccaaact cttatatcac tttttttaaa cagacatata
     5281 ctaaactaac tttagtgaga cccttcacta aagtcaaccc taaaccggag gagggattat
     5341 tgtgggagta cccaacgagg atgtagatgc aaaagcccgt gtctccgtat ttcagagcaa
     5401 tagtgcgaag attttcgcaa gagtatctgc tgaagaggaa gagcggcgcc ggaaaggcca
     5461 cggtgcagca ggcgtagagt aataaacggt tatatagctt gctatgaatg gggctggggt
     5521 aaaacccagc cttcctaagc caatttggca acgattgagt ttttacttct tctgtactgt
     5581 cttctttcaa tcatagtcta tgaagcctgg tcggaaaatg gaatccagtg gttagtgcaa
     5641 tttaaaaatc ctatttcgct gagaacactt tttcttttgg ctttataatg acgcgctaaa
     5701 ttggcaatcc taaataaagg aggggaaaaa atgccgaata ttggtgaaga cccggaagcc
     5761 aaagcacgtg tcaccgttaa tcaacggaat agtgcaactg tcttcgtaag agcatccgcc
     5821 gaagaggaag agcggcgaag acgccggaaa catcaagaat acgaagaaga gtaagctaaa
     5881 acaatcctat tcctccatga taataggctg gggtaaaacc cagcctattt tattactgat
     5941 gccaggatcc gctccgcccg gtcacgagca aaacttatcg tttataacgg tgcatcatgg
     6001 aaatctgctg agaaatatgc aattacaaaa tacatggcgc taatcgtcaa aaaaaaccgt
     6061 cttacgattt taatccgcgt tattctacct tatccggcta acctggtgtc atagcacccg
     6121 actatcgatt tggtcctcca aagggtttgc gttacaatca aatcacaaaa ggtcaataaa
     6181 ggtcaaacat atttggaggt gttattcatg tttaacttaa ctccttttca attcaacgat
     6241 atcgaaagga accttttccc ggcgttcttc ggtgaagagt tcctcgggaa cagtctgggc
     6301 agcttccgta cggatctgca cgataatggc aaggaatatc tgatcgaagc cgagctcccc
     6361 ggttttgcca aggatgagat tcaggtcaaa gttgaagata attctttgac catttctgcc
     6421 caaaggcacg aggccaaaga agagaaacga gataattaca tacgccggga acgctgcagt
     6481 ggccggttag tgcggagctt tgcccttgat ggtgttcaga gtgaagggat caaggcggaa
     6541 tttaaagacg gcatcctaaa gttaa
//
LOCUS       DASVMB010000500         5135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_431192,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000500 DASVMB010000000
VERSION     DASVMB010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431192"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF531_13955"
     CDS             <1..603
                     /locus_tag="VF531_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7715114.1"
                     /translation="MLGQICGITNFLTWIVTDPEPIHKLVHCWLNTSKRYIREMRSAT
                     SYPPEQPGFSFASDVAGMLSPGLYREFIFKAELELYEEFAPGPDDKRFYHADYHMLHH
                     LDAFREMGVNEVNIDPYIDPGQILAKLPQAIVYGQIPPTKVLLYGTPDEVVACAKRDI
                     EQAGPGKHLVLCTAGSINPGTSFENLRALCYAAEKYGYIY"
     gene            890..2986
                     /locus_tag="VF531_13960"
     CDS             890..2986
                     /locus_tag="VF531_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HEX7715115.1"
                     /translation="MADKDQEQTPALGRAGAGYDCWLGYRRLDEAVRLEYQKWCSHIV
                     APDDLGHQNTIFSELLRGVEQMLGVQPDISNEPKSPGSLIIGRFDQSALIDANISEEN
                     KRAIGPEGYCLKTLEGPDQNLLIVTARTDRGLLYGTYALLRLMSTQANLAGMIRIENP
                     RNPLRIIDHWDNMDGSVERGYAGRSIFYYANQLTKDFKRLRDYARLLASIGINGVVIN
                     NVNVKDYETRLITSEYLPDLAKLAAIFRDFGIRIYFSINYASPIRLGNLPTADPLERA
                     VQEWWARKAAEIYSFIPDCGGFLVKADSEFTPGPFTYGRNHAEGANMLAEALEPFGGL
                     VIWRCFVYNCTQDWRDRTTDRAKAAYDNFKPLDGEFRPNVALQIKNGPMDFQVREPVS
                     PLFGGLTRTNQLLELQITQEYTGQQRHLCYLVPQWKETFDFDTFAAGQGTTVQQIVAG
                     SFYKRPHAGIAGVANIGDDPNWTGYDLAQANLYGFGRLAWNPDLTAREIAEEWVGLTF
                     GNHPRVIRVITEMLLDSWKIYENYTVPLGIGWMVNPGYHYGPNVDGYEYSKWGTYHRA
                     DCHGIGIDRTRGTGTGFIGQYHEPNAEIYESLDKCPDELLLFFHHVPYSHRLKSGETV
                     IQHIYNTHFDGVDQVVELRDKWLGLRGLVAEERFTRILQRLEEQLEHAKEWRDVINSY
                     FFRKTGIADELGRKIY"
     gene            3143..>5135
                     /locus_tag="VF531_13965"
     CDS             3143..>5135
                     /locus_tag="VF531_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7715116.1"
                     /translation="MSERPAYQDESLSFRERARDLVARMTLEEKVTQMVYNAPAIPRL
                     GVPSYNWWNEALHGVARAGVATMFPQAIALAATFDADLIHRVAEVIATEARAKFHEFQ
                     RQGDHGIYKGLTFWTPNINIFRDPRWGRGQETYGEDPYLTGRLGVSFIKGLQGDDPKY
                     LKAAACAKHFAVHSGPENERHSFDAVVSKKDLRETYLPAFEESVKEAGVAGVMGAYNR
                     TNGEPCCGSKMLLKDILRGEWGFTGYITSDCWAIKDFHENHGVTSTAPQSVALAVNNT
                     CDLNCGNMYANLLLAYQEGLVDEAAIDESVIRLMTIRMKLGLFDDPSHVPYTNISFEE
                     NDHPHHRQFSLGVAKKSLVLLKNDNNLLPLDKTKLRSIAVIGPNADSREALIGNYFGT
                     PSRYVTVLEGIREAVPETTRVLYAQGSHLYRDKVSGLAEPGDRLAEAVATAQSADLVL
                     LCLGLDAGIEGEEGDTSNEFSSGDKKSLDLPGPQQELLEKIHQTGKPIVLVLLSGSAL
                     AVAWADQNIPAIIQAFYPGAEGGKAVAALLFGEYSPSGKLPVTFYRTTEELPDFRDYS
                     MRNRTYRYMQNEPLYPFGYGLSYTRFEYRDLKLERNSIRIGENVDCSVMVKNIGGYDA
                     EETVQLYLKDVAASVEVPRWQLRGVRKVFLKPGEEAEVHF"
BASE COUNT         1277 a         1180 c         1435 g         1243 t
ORIGIN      
        1 atgttgggtc agatctgcgg catcaccaac ttcttgacct ggatcgtaac cgatccggaa
       61 cccattcata aactggtcca ttgctggcta aacacctcca aacgttatat tcgggagatg
      121 aggtcagcga ccagttatcc tccggagcaa cccggattct cgtttgccag tgatgtggcg
      181 ggtatgcttt caccgggatt gtatcgtgaa tttatcttca aggccgagtt ggaattatac
      241 gaggagttcg caccgggtcc ggacgataaa aggttctacc acgccgacta tcatatgctg
      301 catcatcttg atgccttccg ggaaatgggc gttaacgagg tcaacatcga tccctatatt
      361 gatcccggcc aaatcctggc caaattgccc caagccattg tatacggcca aattcctcct
      421 accaaggtgc tgctttacgg aactccggat gaagtagtgg cctgcgccaa gagggatatc
      481 gagcaggccg gccccggaaa gcatctggta ttatgcaccg ccggaagcat taatcccgga
      541 acatcctttg aaaacttacg ggcattatgc tacgccgcag aaaaatatgg ttatatctat
      601 tgagaggact taagcgagcg gcttattacc ggcagatatg aatggaatta tttgagttaa
      661 aataagatca agtcgggagg gtttttcttg agtaacggct tgccgagtcg ggccgaagtt
      721 ctcttattct gtctaagtcc gggaacgttc ccggagaaat tccttgtatg caaagatgaa
      781 acaaagagca atattgataa ttgttttatc gaaaccggaa gccataatga aaaagaaaga
      841 gaaaattccg tgatgtgttg atgattttta catatagagg gggatggcta tggcggataa
      901 ggatcaagaa caaaccccgg ccttgggccg ggcgggggcg ggatatgatt gctggttggg
      961 ctatcgtcga cttgatgaag cggttcgtct tgaatatcag aaatggtgtt cacatattgt
     1021 cgcaccggat gatctagggc atcaaaacac gatatttagc gagttactgc ggggggtcga
     1081 gcagatgttg ggagtgcagc ctgacatcag taatgaaccg aaaagccccg gttcactcat
     1141 catcggacgt ttcgaccaaa gtgcgctgat tgatgccaat atctccgaag agaataagcg
     1201 ggcaataggt ccggaagggt actgccttaa aaccctggag ggaccggacc aaaacctgtt
     1261 aatcgttacc gcccggactg atcgtgggct gctttatggt acatatgcct tgctccgctt
     1321 gatgtcgacc caggcgaatc tcgccggaat gatccggatt gagaatcctc gtaacccctt
     1381 gcggatcatc gatcactggg ataatatgga cggaagtgtg gaacggggtt acgccgggcg
     1441 ttcaatcttt tactacgcca atcagttgac caaggatttc aaacgcctcc gcgactatgc
     1501 ccgcctcttg gcgagcattg ggattaacgg ggttgtcatc aataatgtca atgtcaagga
     1561 ttatgaaacc cggttgatta cctcggagta cctgcctgat ctcgccaaac tggccgcgat
     1621 cttccgggac ttcggtatcc ggatatattt tagtatcaat tacgccagcc cgatccgctt
     1681 gggaaatctt ccaactgcgg atcccctgga acgggcagta caagagtggt gggcgcggaa
     1741 agcggcggaa atctacagct ttattcccga ttgcgggggc tttttagtga aagcggactc
     1801 cgaattcaca ccgggtcctt ttacatacgg ccggaatcac gccgaaggcg ctaatatgct
     1861 ggcagaggcg ctggaaccat ttggcggctt ggttatttgg cgttgttttg tctataactg
     1921 cactcaggat tggcgtgatc gcactacaga ccgggccaaa gcggcttatg ataattttaa
     1981 gccgttagat ggagaattta ggcctaacgt agcgcttcaa atcaaaaacg ggcccatgga
     2041 tttccaggta cgcgagccgg tttcgccgtt attcggagga ttaacccgga ccaatcagct
     2101 gcttgaattg cagatcaccc aggagtatac tggtcagcag cggcacctgt gttatctggt
     2161 cccccaatgg aaggaaacgt ttgatttcga cacgttcgcc gctggtcaag ggacgacggt
     2221 ccagcaaatc gtagccggtt ctttttataa aaggcctcat gcaggaattg ccggtgtcgc
     2281 caacattggt gacgatccca actggacagg ttacgacctg gcccaggcca acctttacgg
     2341 cttcggccgg ttggcatgga acccagattt aactgcaagg gagattgcgg aggaatgggt
     2401 ggggctaacc ttcgggaatc atccacgggt gatccgggtc atcactgaaa tgctactaga
     2461 ctcttggaaa atttatgaaa attataccgt cccgcttgga atcggatgga tggtcaatcc
     2521 ggggtatcat tatggtccca acgtggacgg ttacgaatat tccaaatggg gaacttacca
     2581 ccgggccgat tgtcacggga tcgggatcga ccggacccgg ggcacgggaa ccggatttat
     2641 cgggcagtat catgaaccca atgccgagat atacgaatca cttgacaaat gccccgatga
     2701 attattactc ttttttcacc atgttcctta ttctcaccgc ttgaaatcgg gtgagaccgt
     2761 gattcaacat atctataaca ctcattttga cggggtcgac caagtggtgg agttacgcga
     2821 taaatggctt ggactgcggg ggttagtcgc agaggaacga ttcacccgaa tccttcagcg
     2881 tctggaggag cagttggaac acgccaagga atggcgggat gtgatcaact cttatttttt
     2941 caggaaaacc ggtatagcgg acgaattggg acgaaagatt tattagaagg catgggtagc
     3001 gcgccccgca gggcgcgaaa aatccggaat tatttgttga gtcatagttg gatcgatgat
     3061 aaaatggtag gaatggcata gatagttaca tcatgattca ggatcaatgt catttttata
     3121 aattggatcc ggagggaata ggatgtccga gagacccgcg tatcaagatg aaagtttgag
     3181 ttttagggaa cgggcccgtg atttggtggc ccgtatgact ctggaagaga aagtcacaca
     3241 gatggtttat aatgcaccgg ctattccccg gctgggggtt ccttcttata attggtggaa
     3301 tgaagccttg cacggcgtgg ccagggcggg ggtggcgacc atgttcccgc aggcgattgc
     3361 cctggctgcg accttcgatg cagacctgat ccatagggtg gcggaggtga ttgctaccga
     3421 agcccgggcg aaattccacg aattccaacg ccagggtgat cacggtatat acaaaggact
     3481 gaccttttgg accccgaata ttaatatctt tcgtgatccc cgctggggac ggggtcagga
     3541 gacttatggc gaggacccgt acctgactgg aagattggga gtgtccttca tcaaaggact
     3601 tcagggggac gatcccaaat acctcaaggc tgctgcttgc gccaagcatt ttgcggtgca
     3661 cagcggtccc gaaaatgagc gacatagttt tgacgcggtg gtctcgaaga aggatttacg
     3721 tgagacatat ctgcccgcgt ttgaagaatc cgtcaaagaa gccggggttg ccggggttat
     3781 gggcgcctat aaccggacta acggcgaacc gtgttgcggc agcaagatgt tattgaaaga
     3841 tattttacgg ggggaatggg gcttcacagg gtacataacc tccgattgtt gggcgatcaa
     3901 ggactttcac gaaaaccacg gcgtaaccag cactgcgccg caatcggtgg ccttggccgt
     3961 gaataatact tgtgatctta actgcggcaa tatgtacgcc aatctgttac tggcctatca
     4021 ggaggggttg gtggatgagg cggccattga tgagtcagtc atccgcttaa tgactatccg
     4081 gatgaaactt ggactattcg acgatccgag ccatgtcccg tataccaata tttcgtttga
     4141 ggagaatgat catccccatc accgtcagtt ctccctgggg gttgcaaaga aatccctggt
     4201 attacttaaa aatgataata accttctacc gctggataag accaagctcc gttcgattgc
     4261 tgtcatcggc cccaatgccg acagccggga ggcattaatc gggaattatt tcggaacccc
     4321 ctccaggtac gtgacggttt tagaaggaat tcgcgaggca gttcccgaaa ccactcgagt
     4381 gctttatgct cagggttctc atttatatcg tgataaggta agtggcctgg ctgagccggg
     4441 agaccggctg gcggaagctg tcgccacagc acaaagcgcg gatttggttc tgctctgtct
     4501 gggtttggac gccgggattg aaggggagga gggagacacc tccaatgagt tcagcagcgg
     4561 cgataaaaag agcttagatc ttcccggacc tcaacaagag ttactcgaaa agattcatca
     4621 aaccggaaag ccgattgtac tggtattgtt atccggcagt gctctggcag tggcttgggc
     4681 ggatcaaaat atccctgcaa tcattcaagc tttctacccg ggggccgagg gcggcaaggc
     4741 cgtcgcggcg ttgttgtttg gtgaatattc tccttccggt aaactgccgg tgacttttta
     4801 ccggaccacc gaagagcttc ccgatttccg ggattattcg atgcggaacc ggacgtatcg
     4861 ctatatgcag aatgagccgc tttatccgtt cggctacggc ttgagctaca cccggtttga
     4921 ataccgggat ctgaaactgg aaaggaattc gatccggatc ggggagaacg ttgattgtag
     4981 tgtaatggtg aaaaacatcg gcgggtacga cgccgaggag acagttcagt tgtatttgaa
     5041 ggatgtggcc gccagcgtcg aggttccccg ctggcagtta cggggagtgc ggaaagtctt
     5101 tctcaaacct ggggaagaag cagaggttca ttttc
//
LOCUS       DASVMB010000501        13824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_576116,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000501 DASVMB010000000
VERSION     DASVMB010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576116"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VF531_13970"
     CDS             <1..702
                     /locus_tag="VF531_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7715117.1"
                     /translation="KGWDFSYLDKRWGSEPLPWDYKLIVSNYLKPSDKLLDMGTGGGE
                     FLLTLNHPNELTGVTEAYPPNVELCKRTLSPLGIDVRQVFDDSEIPFGADEFDVVINR
                     HEAFDVNEVSRILTSGGYFITQQVGSKNNNDLSNKLIDGFSPRFPDHDLKNNIQLLQD
                     LGFAILFSDEAFPTIKFFDVGALVYFAKIIEWEFPKFSVDSCFENLCKAEKELHEHGF
                     IMGTEHRFIIVAQKQ"
     gene            1000..2307
                     /locus_tag="VF531_13975"
     CDS             1000..2307
                     /locus_tag="VF531_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715118.1"
                     /translation="MKKGILIFLMVMLVVSLIGYSAVLGAAKKDTGPLRIMWWGSQTR
                     HDRTLAVMDMYTKNTGVKFEPEFYGFDDYISKLNILIAANDAPDLMQMGGNFPTYLAH
                     IEPLNKYINNHTIDTSNTDKSFIGITTLGGKTVGLSSGTNAPAIAYDPALFKKAGVPL
                     PDIKWTWEDFEKAVLTIHEKLGILGCSLTRADEFWALTTVVSQYGTGESLFLEPYRLK
                     LNYKNNKYVTDYMKMVYRLTKAGAYPNPAQMAEIKDIEGNPLVRGESAIAWLYSNQFV
                     ALSKAAGRPLALTVLPRHSRTGPLSQTIMSSQMFCIAKSSKHKPAAAEFLNFFANSVD
                     ANKILQGERGVPIMRHIREALAPNLSDPEKEVYQYLTNLGKEASTDIVLDSPVQMQIR
                     DLYKSLVEEALFDKITPDEAAKQFKTQAEDALKRYEQQQKQKK"
     gene            2433..3365
                     /locus_tag="VF531_13980"
     CDS             2433..3365
                     /locus_tag="VF531_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715119.1"
                     /translation="MIKLQTKQRSLKNFIYHEAVTGYIFAAPFIIGFLAFTMIPMFYS
                     LFLSFTDYQITATPVWVGLRNYVRMFTDDPRFLKSVGVTLYYVILSVPFKVAFALLVA
                     LLLTRIKPFAGFYRSVYYLPSLIGGSVAVSLVWMELFKVKGVVNTLLAALGMKNPVYW
                     LGDPRFTIFVIVLLTVWQFGSSMIIFAAGLKQIPVSYYESAQIDGGSSLQQFLYITLP
                     CLSPVILFNLIMQTISGFMNFTQVFIITKGEGGPMDSTMFYALYLYKRAFNYFEMCYA
                     SAMAWILLIIIAVVTVLIFKTSDSWVYYESKEGK"
     gene            3367..4221
                     /locus_tag="VF531_13985"
     CDS             3367..4221
                     /locus_tag="VF531_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715120.1"
                     /translation="MSGTMRFKKVLLRILSHGLILFLGLVMIYPVIWMVLGSFKTNYE
                     ILNESLKLMPDTFSLKNYITGWRGFGNLNFGLFFTNSFIITILATIGVAISSSLVAYA
                     FARIRFPGRKLLFSCMMATMMLPAQVVMIPQYIFFRNLDLTNTFVPLILPSWFASAFF
                     VFMMMQFIQGIPRELDEAAIIDGCSKYTIYSRIILPLISPALITTIIINFYWKWDDFM
                     GPLIYLSRPKLFPVSLAIKLFADSTSQTDYGAMFAMSSLSLVPVFLIFLFFNKYLMEG
                     ISTTGLKG"
     gene            4543..4923
                     /locus_tag="VF531_13990"
     CDS             4543..4923
                     /locus_tag="VF531_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715121.1"
                     /translation="MSSKCSVLNDLANLSQTVASFLSSPGTLTPTDITNLVTVVQTAQ
                     GAVRDLPINITRKTDILNRLDQAIFILQNNVFLFNTPTTLLAVLEIFQLVSLKVQNLM
                     LPCPQGIVTVTPSNKFSTLCQICN"
     gene            5273..6241
                     /locus_tag="VF531_13995"
     CDS             5273..6241
                     /locus_tag="VF531_13995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7715122.1"
                     /translation="MRVLFTNDPPLIKYGIAQGFQQLNHEIHIMQGEQERLWGQPLSE
                     QRLRLEQAIEEFKPDFVFTEGHPDFDPLTVCKTISSHKIPHLYWAIEDPVCTNLSTKT
                     YIPYADWVFTTTAECVPFYRSLGRRAELLLFGCNPRFHCFRGKHPEYRHDLVLVATNY
                     SSRYTEAEWLVMPFVERGYDIKIWGLWWDDPDRPIHLQRYPEKFGGLLPYEELPMVYS
                     SAKIILGMNCDSSSITQTSMRPYEALACAGGLYLGHYTKAQDHIFKDRIFQVRNTAEA
                     VRTVNRLLALGETERKAMALKAQREVYQKHSYLQRANQVLRAVAQL"
     gene            6288..7478
                     /locus_tag="VF531_14000"
     CDS             6288..7478
                     /locus_tag="VF531_14000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7715123.1"
                     /translation="MPQYIVSQLPARVNGQIACFSSTHWQFLWQRPQQIMQRLSSYYN
                     ILYVDPPYPVSPVELAEREKQYPENLGARLKSIQNSLKILTPVKLAINADGMDNEDLA
                     RENARLVGAQIRKTLARLHWYQPLLWVYDLAAVPILTELTGRGVVYDCVDSFAAFSWA
                     DPHTSDWERKLLRRADVVITSARTLYQERREENPHIYLIPNAADFEHFAKTSVTTEPG
                     ELRKIPHPRLAFVGAVYEWLDMKLLAKLAQDHPAWSLVMIGPQQSGLELPEVSNLYWL
                     GTREYKLLPWYMQYFEVMLIPFLHNEVTDHANPIKFWEYLAAGKPVVATRLPEIPPIP
                     GVTWLCDNYHQFQEGCLKALKVAADPILRAETSEKARNFARINSWDERCRIIRGILRD
                     HFNM"
     gene            7677..8747
                     /locus_tag="VF531_14005"
     CDS             7677..8747
                     /locus_tag="VF531_14005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7715124.1"
                     /translation="MRIVMLADRLILGGLETHIITFCNELLRRGHRILLYAAYAEPDI
                     IAAIGRNNGNFLYLNWEDRIPDEVRRFNPEIIHSHPFTAIIRGYDLAVMFQKPFFITI
                     HGLYDFGVDRSPLGRQITSQAQRIIAVDQAVASLLRSNTPCPEKVTIIGNGIDLTSFQ
                     PFPPVRIDRAVYGLEPNWYTIVIISRLADGKEGPVRQLIHCAHDLAARLKGLNLLIAG
                     DGPYLEPLRNLATRSAVYPDLRMRLLGKVNDVRGILSLADHVMACDRAALEAMACRRP
                     VYAMNGSGFGGGIGRHNFEKLLGERSGYQVMTDDALISSLYAFVKNKTLRRRLAGEGM
                     AVIHRYFDINNAVSQLEQVYQS"
     gene            8819..9583
                     /locus_tag="VF531_14010"
     CDS             8819..9583
                     /locus_tag="VF531_14010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7715125.1"
                     /translation="MNQVSILLTSFERAPLLRWGLYSLARQHIPFPFETIVINDGLPD
                     ETEMICGQYRERLNLRYIFSGQRNLGGAGIRWRVPGFALNIGAHSATGRVIILSCAEI
                     FHLNETIAQLVQPILENNTLITVPIGKDDRDGSFLHQLASGNGSFDPNTFWNYPELNV
                     RLPFLLAVERAHFYAIRGYDEDFTGIAFDDNDLVDRLVAYGCKYLQTQAQAIHLYHPR
                     YVYQVGESPEWHYNRNLYLSRQGRLVRNENREWGRL"
     gene            9584..10435
                     /locus_tag="VF531_14015"
     CDS             9584..10435
                     /locus_tag="VF531_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715126.1"
                     /translation="MWHLEHIPKLAHFYWGNDTVSYLRYLAVFSFKKLNPDWKIKLYV
                     PKYRYTGAKTWGTSEHSGRFSGPDYTGKLENLGLERVEIDFSHFGIDNMIPETFKADF
                     LRWYLLSTSGGLWSDFDIIYFQPIEALYLNQTGYRALDTTICLQAENGGYYHSIGFLL
                     SSAGNPYYKFISGQTYGMLDLGDYQSIGSRIPNLYFPNALLIRDRFPGINLENLSPDV
                     VYPINYAMVPYIFHTPYMHYLTDQTIGLHWFAGHPESCKFENLLNDDSLAGYNNIISQ
                     VIRRVIA"
     gene            complement(10551..11345)
                     /locus_tag="VF531_14020"
     CDS             complement(10551..11345)
                     /locus_tag="VF531_14020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7715127.1"
                     /translation="MRNYRIVGTCQIYNEIRKENLKRFVKYFMPLLAELVVYDDGSTD
                     GSYEYMLNHTPHVIRGGVNDFINERSHKQILLERALELKPDFILWMDADEVLTANGPE
                     RLPLICDYAMAKGIDGISFHELNLWRSSSWRRMDSLFNDGWFVRLWQVNPGMTYLQIK
                     PGLHQEPYPSTIDRIERETEVQVLHYGFSSEKRLAYKYLTYQSYGQSGYVMLDRLIDE
                     RTLRLEKVSRQLIPKELWRNEPQPTPLSWDESLAYVENFRDEFNQA"
     gene            complement(11390..12400)
                     /locus_tag="VF531_14025"
     CDS             complement(11390..12400)
                     /locus_tag="VF531_14025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7715128.1"
                     /translation="MDDLDLPEFQTKLREILNLPPDTAARYLEALAPLVNRMAQTGLG
                     TTACLETGCLPLPVHFYSPVPDLNDLRNRRIWEKQSTLHGIAWNTQEQLAFLEMLGHY
                     YGSECRWPAKPIGDPNQFYTENNSFSFGCAAITHCLIRYAKPRRLMEIGSGFSSQVID
                     EALMMNRHEGSSCQYTVIDPYPASQLHSGLNSKPMVREERVELVDPEFFSQLQANDIL
                     FIDSGHTVRSGGDVNFLILDVLPRLSEGVMVHFHDIPMPYEYPESYFTNPGFRVFWTE
                     SYLLQAFLCLNPQYRILLAVSDIFHLHRDDFQNAFRYYDPVEHRNASSSFWIQRVGRK
                     QA"
     gene            complement(12433..13497)
                     /locus_tag="VF531_14030"
     CDS             complement(12433..13497)
                     /locus_tag="VF531_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7715129.1"
                     /translation="MSLFQGKVVVITGGTGSLGQVLTRRLLEYPGETPRKIIIFSRDE
                     AKQHQMRITYQQKVKPTDEVIYHNFERLIEFRIGDIRDYSAVRSVLQKADIVINAAAL
                     KQVPSCEYFPFEAVQTNIIGAENIVRAIREAGLQVETVVGVSTDKACKPVNTMGMTKA
                     IQERIWIAANINAPHTRFICVRYGNVLASRGSVIPLFHEEIRNGGPLTVTTPEMTRFL
                     LPLERAVDTIFAALAEARPGEIYIPRIASARITELAKVLMADRRLPLIYTGIRPGEKV
                     HEILVSEEEAGRTFERGRYLAIQPMLPEVTATDFNVVPLGKAYSSADVLMSPETIRSL
                     LVEQHLMVDQIESEGIEVLR"
BASE COUNT         3432 a         3340 c         3347 g         3705 t
ORIGIN      
        1 aagggctggg atttttcata cttggacaaa agatggggat cagagccgct cccgtgggac
       61 tacaaactta ttgtatcaaa ttatcttaaa ccgtcggata aattattaga tatgggtacc
      121 ggcggtgggg agtttttgtt gacactaaac catcccaacg agttaaccgg tgttaccgaa
      181 gcatatccgc ctaatgtgga gttgtgcaaa cggactttat ctccactagg tattgatgta
      241 aggcaggttt ttgatgacag tgagattccg tttggagcag atgagttcga tgtggtcatc
      301 aatcgtcatg aggcttttga tgtaaatgaa gtaagtcgca tactgacaag cggaggctat
      361 tttatcacac aacaggtggg aagtaaaaat aataacgact tgtcaaataa actgatagat
      421 ggcttttccc cgcggtttcc agaccatgat ttaaagaata atattcaatt gttacaagac
      481 ctgggatttg ccattttatt ctcggacgag gcgtttccaa caattaaatt ctttgacgtt
      541 ggcgcattag tatatttcgc aaaaataatc gaatgggagt tcccaaagtt ctcggttgac
      601 tcttgttttg agaacttatg caaagcagag aaagaattac atgagcatgg gttcataatg
      661 gggacggagc atcgtttcat aatagttgca caaaaacaat gacatgtacg taatctttag
      721 tttatgatct tggatagaag gtaaagagag tatagggtac caaggccgca agcgatttcg
      781 tttgcggtct tttcattagc tacttttgct gcccggatgc ttgggatccc ggctcaaggt
      841 tttggtatct cattttcata attggtatca tattttcata atggtatcac actttattaa
      901 ttggtatcca ttcttggaaa tattggggtt tgccgagctt tggttaccat gattcggtat
      961 aatataaatg taacaaaaaa gtaaagagga ggttagttca tgaaaaaagg gattttgatt
     1021 tttcttatgg taatgctggt tgtttccttg attggctact cggcagtact gggagcagca
     1081 aaaaaagata ccggaccgct gcggatcatg tggtggggat cgcagacccg gcatgatcgg
     1141 accctggcag tgatggacat gtataccaaa aacaccggcg tcaagtttga accggagttc
     1201 tacggctttg acgactatat ctccaagctg aacatcctga tcgccgccaa tgacgctccg
     1261 gatctgatgc agatgggcgg gaacttccct acttatcttg cgcacatcga gcctttgaat
     1321 aaatatatca ataatcatac catcgatact tccaatacgg ataaaagctt tatcggcatc
     1381 acgacgttag gcggaaagac ggtcgggctc tcttccggga cgaacgctcc ggccattgcc
     1441 tacgatcccg ctttatttaa aaaagccggg gtgcccttgc ctgacatcaa atggacctgg
     1501 gaagattttg aaaaggctgt gcttacgatc cacgaaaaac tggggattct cggttgcagc
     1561 ctgacccggg ccgatgaatt ctgggctctg accactgtgg tttcccagta cggcaccggc
     1621 gagagtcttt tcctcgagcc ttaccggctt aagctgaatt acaaaaataa caagtatgtc
     1681 accgattata tgaaaatggt ttaccggctg accaaggccg gcgcttaccc caatccggcc
     1741 cagatggctg agataaagga cattgaagga aacccgctgg ttagagggga atccgccatc
     1801 gcctggcttt acagcaacca gtttgtggcg ttgtcgaaag ccgccggccg cccgctggct
     1861 ctgaccgtcc tgccgcgcca ttccagaacc ggcccgctat cccagacgat catgtcgtcg
     1921 cagatgttct gcatcgccaa gagttccaag cataaaccgg cggccgccga attcctgaat
     1981 ttctttgcca acagcgtcga tgccaacaag atcctgcagg gtgaacgcgg tgtgcccatt
     2041 atgagacata ttcgtgaagc cttggctccg aatctttcgg atccggaaaa agaggtttat
     2101 caatacctca ccaatctcgg taaagaggca tccaccgata tcgttctgga ttccccggtc
     2161 cagatgcaga tccgggatct ttacaaaagc ctggttgagg aagccctgtt tgataaaatc
     2221 acccctgatg aagcggctaa acagtttaaa acccaagccg aggatgctct gaaacggtat
     2281 gaacaacaac aaaaacaaaa gaaataatcc ggtaacaccc cgtatttgtt cgtagtgtaa
     2341 aatcagccaa tgcggaacat gccccaattt gcaagttggg gcatgtttat aatgaaaaat
     2401 gacatattgc gacttaaagg ggttgtaatg taatgatcaa gctacaaacc aaacagcgaa
     2461 gcctgaaaaa tttcatctac catgaagctg tcaccggcta tatttttgct gcgccgttta
     2521 tcatcggttt cctggctttt accatgatcc ccatgtttta ttcattgttt ctatccttta
     2581 cggattatca gattacggcg actccggtat gggtcggtct acggaattat gtccggatgt
     2641 ttacggatga cccccgtttt ttgaagtcgg tgggcgttac tctctactat gtgatactct
     2701 cggttccatt taaggtcgcc tttgcgctgc tggtcgccct gcttttaaca cggatcaagc
     2761 cctttgccgg tttttaccgt tcggtctatt atctgccttc gttgatcggc ggcagtgtcg
     2821 cggtatcgct ggtctggatg gagctgttca aggtgaaggg cgtggtcaat accctactgg
     2881 ccgcgctggg tatgaagaac cccgtctatt ggctgggaga tcccaggttt accatctttg
     2941 tcatcgtact gttaaccgtc tggcagttcg gttcttccat gatcatcttt gccgccggct
     3001 tgaaacagat accggtcagc tactatgagt ccgcccaaat cgacgggggg tcatcgctgc
     3061 agcagtttct ctacatcacc ctgccctgtc tctccccggt gatccttttc aatctgatta
     3121 tgcagaccat ttccggtttc atgaacttca cccaggtatt tatcattacc aaaggtgagg
     3181 gcggtccgat ggacagtacc atgttttatg ccttatatct ctataagcgg gccttcaact
     3241 attttgaaat gtgctacgcc agcgccatgg cctggatact gctgatcatc atcgccgtag
     3301 tgaccgtcct gattttcaaa acctccgaca gttgggttta ttacgagtcc aaggagggca
     3361 aatgagatgt ccgggactat gcgattcaaa aaagttctct taagaattct ttcccatggt
     3421 ctgatcttgt ttttggggct tgtgatgatc tatcccgtga tctggatggt tttgggctct
     3481 ttcaagacca actatgaaat attgaatgaa tctctgaagc tgatgcccga caccttcagc
     3541 ctgaagaatt acatcacagg ctggcggggt tttggtaatc tcaactttgg gctgtttttc
     3601 accaattcct ttattatcac tattctcgct actatcggag tggcgatctc ctcctcgctg
     3661 gtggcttatg ccttcgcccg gattcgtttc cccggccgga aactgctctt ttcctgcatg
     3721 atggctacga tgatgctccc ggcgcaggta gtgatgatcc cgcagtatat tttcttccgc
     3781 aatctggatc ttaccaatac ctttgtcccg ctgattctac cgtcctggtt tgccagcgct
     3841 ttttttgtct ttatgatgat gcagttcatc cagggaattc ccagagagct tgatgaggcc
     3901 gcgattatcg acggttgctc gaagtatacg atttattccc ggattattct gccgttgatc
     3961 agtccggcct tgatcaccac gatcatcatt aatttctact ggaaatggga tgacttcatg
     4021 gggccgctga tctatctgag ccggccgaag ctgttcccgg tttcactggc gatcaaactg
     4081 ttcgccgatt ccacctccca gacggattac ggcgccatgt ttgccatgtc atccctgtca
     4141 ctggtgccgg tcttcctgat ctttttgttc ttcaataaat acctgatgga ggggatcagt
     4201 accaccggac taaagggata agcgagattc catcccggtt tgtcctgaaa cggcaataaa
     4261 aaaagggcgg agctcccgcc cttttttttg tttttcttcc atatctttac cccgaatccg
     4321 gcaaggaatt taaaaattca tacctgataa gtactgaata agccgcggta agaattcctt
     4381 ccggccgggg acatcggcac cccatcacct acacttcaaa atccggtttc ggtttttccg
     4441 cctatgttcg gaagacagat cttcccgtga aaccagcccg ttgttatgga ggtatagctc
     4501 cccttcgtcc gggatcacat aatataaagc ggaggtgata cgatgagcag taaatgttcg
     4561 gtattgaatg atcttgccaa cctctctcaa acagtggctt catttttgag cagtcccggc
     4621 actctgacac cgactgatat cacgaatttg gtcacagtgg tccaaacggc acaaggagcg
     4681 gtaagggatc ttcccatcaa catcacccgg aagaccgata tcctaaaccg gttggatcag
     4741 gctatattta tactacaaaa caatgtattt ctctttaata cccccaccac acttctggca
     4801 gtactcgaaa tcttccagtt ggtatcgttg aaggttcaaa atctgatgct tccttgcccg
     4861 caaggcattg tcaccgtgac tccctccaat aaattcagca ccttatgcca aatctgcaac
     4921 taatatcaac ttgaaatgcc ggcaggttta aactcaatgg tccgcagcgg tttcccggaa
     4981 ttatcttgtt tttcttcaac ttcagcatga attttttacc gaagggacct aaagcagcgg
     5041 atccagatca agatacagcc tgtagtcgaa tcaagccgct ggtggtaggt tattctctcc
     5101 ggcccggtca atgaccgggc tttcattatg acgctaccag acaattcact cctgacctgc
     5161 cgggggagtt tgggactttc gccataccac catttggaat atgcgggaat aacataagaa
     5221 taaagccggc ttgcgaaaca atactttttt ctaaaaagca aggggagtgg atatgagggt
     5281 tttgttcacg aacgatcccc cgttgattaa gtacgggatc gcccaagggt ttcaacaatt
     5341 gaatcatgag atccatataa tgcaaggaga acaggaacgt ctatggggtc aacctttatc
     5401 ggaacaacga cttcgtttgg agcaagccat tgaagaattt aagccggatt ttgtatttac
     5461 cgaaggccat cccgacttcg atcccctaac ggtctgcaaa acgatttcca gccataagat
     5521 accccatctt tactgggcga ttgaagatcc ggtatgtacg aacctttcca cgaagaccta
     5581 tattccgtac gccgattggg tatttacaac cactgcggaa tgtgtccctt tttaccgctc
     5641 cctcggcagg agggcggaac tgttgctgtt tggatgtaat ccgcgttttc attgttttag
     5701 gggtaaacat cccgaatacc gtcacgattt agttttagta gccaccaatt attcttcgcg
     5761 atataccgag gctgagtggt tggtgatgcc gtttgtggaa cgtggttacg acattaagat
     5821 ctgggggttg tggtgggatg atccggatcg ccccattcat ctgcagcgat atcctgagaa
     5881 attcggtgga ttgcttccat atgaggaatt gcctatggtc tacagttctg ccaagattat
     5941 tctcgggatg aattgtgatt cctcttcgat aacccagaca tccatgcgac cctacgaggc
     6001 tttggcatgt gccggcggtc tatacctcgg tcattatacc aaggctcagg atcacatttt
     6061 taaagatcgg atctttcagg tccgaaacac ggcagaggct gtaagaacag ttaatcgcct
     6121 actcgccttg ggtgagacgg aacgaaaggc catggcgctc aaagcacaaa gggaagttta
     6181 ccaaaagcat tcctacctgc aacgggctaa ccaagtttta cgggctgtgg cccaactata
     6241 gtggttttgg aagaatgatt cgagattacc acagaggtga attccatttg ccccaatata
     6301 ttgtcagtca actgccggcg cgcgtcaatg gtcaaatcgc atgcttctcc tctacccatt
     6361 ggcagttttt gtggcaacgt cctcaacaga tcatgcagcg cttaagcagt tattataata
     6421 ttttgtatgt cgatccgccg tatccggttt caccggttga acttgctgaa cgcgagaaac
     6481 aatatccgga aaatctcgga gcgcggctaa aatcaatcca aaactccctt aagattttga
     6541 cccccgtaaa actggccatc aatgcggatg gaatggataa tgaagatctg gcccgggaaa
     6601 atgccaggtt ggtaggggcg caaatccgga aaacattagc gcggctccat tggtaccaac
     6661 cgttactctg ggtgtacgat ctggctgcgg tcccaatcct cacggaactt accggacgag
     6721 gagtggtcta tgattgtgtc gatagcttcg ccgcctttag ttgggcagat ccacatacca
     6781 gcgattggga acggaagtta ttacgccgcg ccgatgtggt tattacttct gcccgtactc
     6841 tctatcagga acggagagag gaaaacccgc atatctattt gattcccaat gcagctgatt
     6901 tcgaacattt cgctaaaacc agcgttacta cggaaccagg cgaattacga aaaatacctc
     6961 atccccgtct ggcatttgta ggcgccgttt acgaatggct tgatatgaaa ctccttgcca
     7021 aactggctca ggatcacccg gcctggagtt tggtgatgat tggcccccag caaagcgggc
     7081 ttgagcttcc tgaggtttcc aacctttact ggctgggaac ccgggaatac aaacttcttc
     7141 cctggtatat gcagtatttt gaagtgatgc tcattccgtt ccttcacaat gaagtcactg
     7201 atcacgcaaa tcccattaaa ttctgggagt acctggcggc cggaaaaccg gtggtggcta
     7261 ccaggctgcc ggagatccct ccgattccgg gggttacatg gttatgtgac aattaccacc
     7321 aatttcaaga aggctgcctc aaagctttga aagtagcggc ggatccaatt cttagggcgg
     7381 aaacttcaga aaaagcccgt aattttgccc gaatcaactc atgggacgaa cggtgccgga
     7441 tcatccgggg aattctcaga gatcacttta atatgtaatc ccctcgcctc catgtggagg
     7501 ggaaaaacct ctcaggaaga gtcgcttcgg agaacggatc atcctgaaaa gggcggaaag
     7561 aacgcctggt tatgttattc gaataacacc gccagtccta aatgtacata gattatctgg
     7621 aacagaattc gggcgggaca cgaatcccca gattcgatga tgccggggga attggcgtgc
     7681 gaattgtcat gttggcggac cgcttgattt taggtggtct agagacccat atcatcacct
     7741 tctgtaatga gttactccgc cgtggccacc ggatcctttt gtacgcggct tacgccgagc
     7801 cggatattat cgcggccatc ggtaggaata atggtaattt cttatatctc aattgggagg
     7861 ataggatacc ggatgaggta cgccgcttta atccggagat cattcattcc catccgttta
     7921 cagcgatcat tcgggggtat gatctggcgg taatgtttca gaaacccttc ttcattacca
     7981 tccacggctt atacgacttc ggagtggatc gttctcccct cggaaggcaa atcacatccc
     8041 aagcccaacg gattatcgcc gttgatcagg ctgtggcatc cttacttcgc agtaatacgc
     8101 catgtcccga aaaggtcacc atcatcggaa atgggatcga cttgacctct tttcaaccat
     8161 ttcctcctgt ccgtattgat agagctgtat acggattgga gcccaactgg tataccattg
     8221 ttattatcag ccgcctggcc gacgggaagg aaggaccggt ccggcagttg attcattgtg
     8281 cccacgattt agcggcaaga ctcaagggac tgaatttgtt aatcgccggc gacgggcctt
     8341 atctggagcc attgaggaat ttagcaacca ggagcgcggt ttatcctgat ttacggatga
     8401 ggttgttagg taaagtcaac gatgtccgag ggattctatc ccttgccgac catgtgatgg
     8461 cctgtgatcg ggcggcattg gaagccatgg cttgccgtcg gccggtctat gccatgaatg
     8521 gctcaggctt tggcggcggg atcggccggc ataattttga aaaattactt ggggagcgct
     8581 ccggttatca ggtaatgacc gatgatgcgc ttatttccag cctatatgcc tttgtaaaga
     8641 ataaaaccct gcggcggcgt ttagctgggg aaggaatggc ggttatccat cgctattttg
     8701 atattaacaa tgccgtttcc caattggagc aggtgtatca atcctaacgc ggcttctcac
     8761 ggactccccg ggcatcgccg tgtggcaaac gaagattagc caaagaggta gcgctatcat
     8821 gaatcaggtt tccatcctgc ttaccagttt tgagcgtgcc ccgctacttc ggtggggctt
     8881 atattcctta gcccggcaac atattccttt tccgtttgag acgattgtca ttaatgacgg
     8941 gcttcccgat gagaccgaga tgatctgcgg gcagtaccgt gaacgattga atttgcggta
     9001 catcttttca ggccaacgaa atctgggggg cgccgggatt aggtggcgtg ttccgggatt
     9061 tgccctgaat atcggggcgc actctgccac aggccgggta atcattctgt cttgcgccga
     9121 gatatttcat ttaaacgaaa ccattgccca actggttcaa ccgattttgg agaacaacac
     9181 gctgattacc gtgcctatcg gcaaggatga ccgtgacggc tcatttctcc accagttagc
     9241 cagcggcaac gggagcttcg atcccaatac attttggaac tatccggaac ttaatgtccg
     9301 gcttccgttt ttactggctg tcgagagggc tcatttttac gcgatccggg gttatgatga
     9361 agattttacc ggaatcgcct ttgatgacaa tgatctggtg gatcggttgg tcgcatacgg
     9421 ctgtaaatat cttcaaaccc aggctcaagc catacatcta tatcatccgc gctatgtcta
     9481 ccaagtcggg gagagcccgg aatggcatta taaccgcaac ctatacctgt cacgtcaggg
     9541 tcggttggta cgcaatgaaa atcgggagtg ggggagatta taagtgtggc atttggaaca
     9601 tattccgaag ctagcccatt tttattgggg aaacgatacg gtctcgtatc tacgttactt
     9661 ggcggttttt tccttcaaaa aactaaatcc ggattggaaa atcaaactat acgtaccaaa
     9721 gtaccgttat accggagcca aaacctgggg gacctccgaa cactcgggcc gcttctccgg
     9781 accggactat accgggaaac ttgagaacct gggtttggag cgggtagaga ttgacttctc
     9841 tcattttggg atcgacaata tgatcccgga aaccttcaag gccgattttc ttcgttggta
     9901 tctcttatca accagcggcg gtttgtggtc cgactttgac attatctatt ttcaaccgat
     9961 tgaggcatta tacttaaatc aaacgggata tagggctttg gatacaacca tctgtttaca
    10021 ggcagaaaat ggtggatact accattcgat cgggtttttg ttgagttcag cgggaaaccc
    10081 gtattacaaa tttatttccg gccaaacata cggcatgctt gatttggggg attaccagag
    10141 catcggctcg cggatcccca atttgtattt cccgaatgca ttactgattc gagatagatt
    10201 ccccggcatc aacctggaaa acctcagccc cgatgtagtg tacccaatta actatgccat
    10261 ggtcccttat atttttcaca caccgtacat gcactatctg accgaccaaa ccatcggtct
    10321 tcactggttc gccggacatc ccgaatcctg caagtttgag aacctgctga acgatgatag
    10381 tctagccggg tataacaata ttatttccca ggttattcga cgtgtcattg cctgatagtc
    10441 aaaaaccatt aagccatctc attttacagg tacgggacat gaataatcat gtaactaagt
    10501 gataaaaaaa cttaaaagcc cagtgaaact gggcttcatt tatgcctcga ttaggcttgg
    10561 ttaaactcat ctcggaaatt ttccacatag gccagcgatt catcccagga aagtggtgtg
    10621 ggttggggct cgttgcgcca taattccttc gggatgagtt gcctggagac tttttccagt
    10681 ctcagggtcc gctcgtcgat tagccgatca agcatgacat aaccgctttg gccatatgat
    10741 tggtaggtta ggtacttata agccagccgt ttctcggaag aaaaaccgta atgcaatacc
    10801 tgaacttccg tttctcgctc aatacggtcg atggtggacg gatacggttc ctgatgcaat
    10861 cccggcttga tctgcaggta agtcatgccc ggattgacct gccataaccg tacaaaccaa
    10921 ccgtcattaa aaagactatc catcctgcgc caggaggaac tacgccagag gtttagttcg
    10981 tgaaagctta tgccatcgat cccttttgcc atcgcgtaat cacagattaa cggcaagcgt
    11041 tccggtccat tggcggtcaa cacctcatcg gcatccatcc agaggatgaa atccggctta
    11101 agctccaggg ctctttccag taagatttgt ttatgactcc gttcattgat aaagtcgttt
    11161 accccaccac gaatcacgtg cggggtatgg tttagcatat attcgtaact tccatcggtg
    11221 ctgccgtcat cgtagaccac caactccgca aggagtggca tgaagtattt tacaaagcgt
    11281 ttcaggtttt ccttacggat ttcattatag atctggcaag ttccgacgat tcggtagttc
    11341 cgcatggcgt ttcctcccct ggcgcgtgcc ttccatgagg ctcccatggt caggcctgtt
    11401 tccggccgac cctttgaatc caaaagctgc tactggcatt gcggtgctct accggatcat
    11461 aataccgaaa agcattctgg aaatcatccc ggtgcagatg gaaaatatca ctcacggcga
    11521 gtaaaatccg atattgggga ttaaggcata aaaaagcctg taacagatag gactctgtcc
    11581 agaaaacccg gaacccggga ttcgtgaaat agctttcagg atattcgtag ggcatcggga
    11641 tatcatggaa atgaaccatg actccttctg aaagcctggg taggacatcc aggatgagaa
    11701 aattgacatc accgccagag cgcaccgtat gtccggaatc aatgaaaaga atatcgttgg
    11761 cctgaagttg gctgaagaat tcgggatcca ccaactcgac ccgttcctcc ctcaccattg
    11821 gctttgaatt gaggcctgag tgaagctgtg aagcgggata gggatcaatc accgtatact
    11881 ggcaactgct gccctcatgg cgattcatca ttagagcctc atcaatcacc tgggaggaga
    11941 agccagatcc gatctccatc aaccggcggg gtttggcgta acggatcagg caatgtgtga
    12001 ttgctgcgca accaaaacta aacgagttat tctcggtgta aaattgattc ggatccccga
    12061 tgggttttgc cggccagcgg cattccgatc cgtagtaatg gccgagcatt tccagaaagg
    12121 ccagctgttc ctgggtgttc catgcgattc cgtgcagagt actttgcttc tcccagatcc
    12181 ggcggttccg cagatcattc agatcgggaa ccggagaata aaaatggacc ggaagtggta
    12241 aacagcctgt ctccaggcat gccgtagttc ccagaccggt ctgagccata cggtttacca
    12301 gaggagccag ggcttcaaga taccgggccg ctgtatccgg tgggaggttt agaatctcac
    12361 ggagtttcgt ttgaaattcc ggaagatcca agtcatccat gaatgaatca cctcttttgg
    12421 ataatttttc gcttagcgta aaacttcgat cccttccgat tcaatctggt caaccattag
    12481 atgttgttcg accaataagc ttcgaatcgt ttccggagac atcaggacat cggcggaact
    12541 gtatgctttg ccgaggggca ccacattgaa atctgttgcc gtaacttcag gaagcatcgg
    12601 ttgaatcgcc aggtaacgtc cgcgttcaaa cgtacgcccg gcttcctcct cggagaccag
    12661 gatttcatgg actttttctc cggggcggat cccggtatag ataagcggca acctacgatc
    12721 ggccatcagc actttggcca gttccgtaat acgggctgag gcaattcggg ggatgtatat
    12781 ctcgcccgga cgagcctcgg ccaaagctgc aaagatcgta tctactgccc gttccagagg
    12841 taaaaggaat cgggtcatct cgggcgtggt tacggtaagc ggaccgccat tcctgatctc
    12901 ttcatgaaaa agcgggatta ccgaaccgcg ggaggcaagc acattgccat aacgaacgca
    12961 gataaaacgg gtgtggggag cattaatgtt ggccgcgatc caaatccgtt cctggattgc
    13021 cttggtcatc cccattgtat tcacaggttt gcaggcctta tcggtggata cgcccactac
    13081 ggtctcaacc tgcagtccgg cttcacggat ggcgcgcacg atattttccg cgccgataat
    13141 atttgtttga acagcctcaa aagggaagta ttcacatgaa gggacttgtt tcagcgccgc
    13201 ggcgttgatg acgatatcag ccttttgtaa aaccgatcgg accgcggaat agtcccggat
    13261 atcgccgatc cggaattcaa tcaatcgttc aaaattatga tagatcacct catcggtggg
    13321 ttttactttc tgttggtatg taatgcgcat ctgatgctgt ttggcttcat cccgtgagaa
    13381 aatgatgatt tttcggggag tctctccggg atactccaat aatcgcctgg tcaatacctg
    13441 cccgagggat ccggttcccc cggtgattac gaccaccttg ccttgaaaca atgacatggc
    13501 attgcctcct atactgaaaa cgccgcccaa gaatggttat tcattcctat atgaatacag
    13561 gttaaaggca atcgatatca ctgaattgca ctttccagtt ctgtttaata tttgccggtt
    13621 tccgcacgga ggtactccgg attggctgta caccggctag agttgtgttt ttgaataaca
    13681 tgaagcggtc tgaaaatccc ggtacatggt ttggatcagc ccttgccagc ctggagcttg
    13741 atatccggtt gattcccgga aacgcccggg attcaatgaa cggtccaatg ccggaccggg
    13801 ttccggtttc acagtgaccg gata
//
LOCUS       DASVMB010000502         8087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_576125,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000502 DASVMB010000000
VERSION     DASVMB010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576125"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF531_14035"
     CDS             <1..542
                     /locus_tag="VF531_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715130.1"
                     /translation="PIAMITQNTKCFADLPVWEFLQIHARARGIQDPGIIADTIEVAN
                     IFTGEKITENYKVTTLSGGQTRSLLIADAILIGAAPIILLDEIENAGIFKSEVMSIIQ
                     DAGKIIIFVTHDPVIALHTRKRLIMENGGVKKVLYQNELEVYAARNLVELDKRIGVIR
                     EELRAGNVITRELVNLNLA"
     gene            555..1265
                     /locus_tag="VF531_14040"
     CDS             555..1265
                     /locus_tag="VF531_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7715131.1"
                     /translation="MKLIIIAGPPSAGKTALVKQIVANLQHELKIAFLKIDVVKAYED
                     VELENEYGILTKKVYSGDLCPDHAGVMVLGDAVEWAGQNGVDLFIVESAGLCLRCSPY
                     LNQGLGIVVLSSISGIHAPEKMGAMVSLADVAVVTKIDLVSQAEREVLIQKIREIHPR
                     VILLETNALQGTSLQRLYDLIRHSADVDPEQLVLKGNPPLGTCTICVGKKDIGWKKHF
                     GVVKRLEGAIADYLYRGE"
     gene            1268..2002
                     /locus_tag="VF531_14045"
     CDS             1268..2002
                     /locus_tag="VF531_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7715132.1"
                     /translation="MGKRTGPEEIRAEQPIETEIHSEKEQRLAPESWAPPGRNCGLCG
                     SQNCGEFLEQVTTAQKRYNDCPFYHKQETVRAPNRGLEVQEALYDGRDVLGHRYDFVL
                     APLPGEISARKIVLPFRSDLVDKLAIGKGDLVVGRPMGAGCPVPHVLRVIEASPITGL
                     LTTWVVGPQYARDHPVKEVDAYHMVGFEGLAVQIEKQPEFGCRVTFLPGFCMMNLNHT
                     GLVNMLLKQTEGYHVRIEDIRILAGI"
     gene            2416..2865
                     /locus_tag="VF531_14050"
     CDS             2416..2865
                     /locus_tag="VF531_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7715133.1"
                     /translation="MQVSTRGRYGLRAMVDIALHITDGPMALRVIAERQGISESYLEQ
                     VFTNLRKAGLVKASRGSQGGYELNHPPQEITVGQILRSLEGPIVPVFCVDESSSGNIC
                     ERSKICPTRSFWEELRDKINEFLDGTTLQDLADRASAAMPEEPMYFI"
     gene            complement(3020..4429)
                     /locus_tag="VF531_14055"
     CDS             complement(3020..4429)
                     /locus_tag="VF531_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HEX7715134.1"
                     /translation="MMNAFKQIINPLAYHTPEDMLFGRAPRPVTGRNGMVIGGGTVYP
                     EINFTLPPMPVESGTIPDILKEYRQIIQGVCERAVVLHSPGIVVEVELLPPMTVHPQW
                     GLDVVKVVKDTLISYEAKQGLKSLLRVTPNDTREMKRPPLLYSGLYWENMLVTFEESA
                     KAGADFLAIESTGGKEICDDALMNGDIASVIFALGVLGAYDMKRLWQEIVAIATKYNC
                     IASGDTACGFGNTAMILAEQGYISKVFAAAVRVATVPRSLIAYEAGAVGPSKDCAYEG
                     PYMKVIAGVPIAMEGKSSACAHLSPLGNIAGAICDLWSNESVQNIKLLGGMAPTVSME
                     QLIYDTRLMNQASQEGFEAAVMLRDWMVESDSRFDPQAYVLRPDFVFQVSRELMTAAD
                     PYRRTKKAVSTALQLLREGVDEGRLALSKREQPYLERLSEQLAGLPDDPAALWENLRG
                     SIPEGRILPSEYQLAEIIG"
     gene            4811..5584
                     /locus_tag="VF531_14060"
     CDS             4811..5584
                     /locus_tag="VF531_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX7715135.1"
                     /translation="MMADSKMHRFSRTELLIGAEGVAALSRARVAVIGLGGAGSYAVE
                     ALARAGVGWLVLADPRMVDISDTNRQLTAAGDAYGKPKVDVLAERVKAINPEAVVVTW
                     QEWAGPWNLEAILHGKVQWMLDTCGLPLQRAERIGFAAGRGIPVMAILDTGGRTDPLS
                     FRKSDIGSIRDCQEAEALRVALYQRGIKTGVSAVYSTEPPVRQTTVAESGCHGSACQK
                     RSSCRGCSGCRQPDGSLSYLAPMAGLIAAGELIRTLQTV"
     gene            5961..7481
                     /locus_tag="VF531_14065"
     CDS             5961..7481
                     /locus_tag="VF531_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7715136.1"
                     /translation="MSDFLRKSPSVKIGIGLVLSLLLVAMAASVTMAAPDPTGAATLQ
                     KDPNAPVNFIWTLICGFLVIFMMTGFAMVETGFTRAIHANNTMLMNMMIWVFGTLGYF
                     LVGFGLMFGGVGGVYEGLSKALSINIGGHTWNLLGLSGFALGGKAYDVGIFMLFLFQA
                     AFMDTTATIPTGAMAERFKWSAFIVYGFLVGGIIYPIFGNWAWGGGWLSQLNVLGLGA
                     GYKDFAGSGVVHAVGGLTALAGTIIMGPRIGKFVKGKPVAMPGHDLSMGVIGTCILIF
                     CWFGFNAGSTMAATDLRLTVAAVNTMLAGCAGGLTAMLYMMKSTGKPDTSMSCNGVLA
                     GLVAITAPCAYVPAWAAVVIGGIAGVLVCASVWFVENVLKLDDPVGAVSVHGVNGMWG
                     VLSVGLFADGTYGGVKGLLLNGDWGQLGAQVVAILALILLIFPFAFLVLKLIDKTIGL
                     RVSAKEELAGLDMPVHGALCYPEFALSALGTAQTFPEEDSQASRTAVKSAAVSKEA"
     gene            7486..7824
                     /locus_tag="VF531_14070"
     CDS             7486..7824
                     /locus_tag="VF531_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7715137.1"
                     /translation="MIKIEAIFRPSRFEAVKDALAKVGIRGMTVSEVKGCGKQKGAVE
                     HYRGSEYEIVLHPKVKIEIVTLEENMEKIITAIQYAAQTGETGDGKIFIYQLAEAIQV
                     RTGERGKNVI"
BASE COUNT         1925 a         1863 c         2256 g         2043 t
ORIGIN      
        1 aaccgattgc gatgattacc caaaacacta agtgcttcgc ggatcttcca gtatgggagt
       61 ttttacagat ccatgcccgg gcccgtggca tccaggatcc cggtatcatt gccgacacga
      121 ttgaagttgc caatatcttt accggggaaa agatcaccga gaattataag gttacgactc
      181 tatccggagg ccagacccgt tcactgttga tcgccgatgc catcttgatt ggcgccgcgc
      241 ccatcatcct gctggatgag atcgaaaacg ccgggatttt taaatccgaa gtaatgtcga
      301 tcattcagga tgcaggtaaa attattattt ttgtgaccca tgatccggtt atcgccctgc
      361 ataccagaaa acgtttgatc atggagaacg gtggtgttaa aaaagtttta tatcaaaacg
      421 aattggaggt ctacgccgcc cgaaatctgg tggaactcga caaaagaatc ggcgttattc
      481 gcgaggaact ccgggccggc aatgtcatta cccgggaatt ggtcaatctg aatttagcct
      541 gaggagcggt gaaaatgaaa ttgatcatca ttgccggccc gccatcggcc ggaaagacgg
      601 cattggtcaa acagatcgtt gccaacctcc aacacgaact taagatcgcc tttctcaaga
      661 tcgatgtggt taaggcgtat gaagacgttg aacttgagaa cgaatacgga atcctcacca
      721 aaaaggttta ttcaggcgat ctctgtcccg atcatgccgg ggtaatggtg cttggagacg
      781 cagtggaatg ggccgggcaa aatggggtgg atctttttat tgtcgaaagc gccggtctct
      841 gtctgcgttg ttccccttac cttaatcaag gcctgggtat cgtggtttta agttccatct
      901 ccggtatcca tgccccggaa aagatgggtg ccatggtcag cctggccgat gtagccgtag
      961 ttacaaagat tgatctggtt agtcaggccg aacgggaggt tttgattcaa aagatccggg
     1021 agattcaccc ccgggtgata ctccttgaga ccaacgcttt acagggaaca tccctacaac
     1081 gcctctacga tttgatccga cactcagcgg atgtcgatcc ggaacagttg gttttaaaag
     1141 gaaatcctcc cttgggaacc tgtactatct gtgtgggcaa gaaggatatc ggctggaaaa
     1201 agcatttcgg ggtggtcaaa cggcttgagg gtgcgatcgc tgattatctc tatcgaggtg
     1261 aatgaggatg gggaaacgaa caggccctga agagattagg gcggagcaac caatcgagac
     1321 tgaaatacat tccgaaaagg agcagcggtt ggcacccgag agttgggcgc cgccgggcag
     1381 aaattgcggg ctttgcggca gtcaaaactg tggggagttc ttggaacaag ttactacagc
     1441 ccaaaagaga tacaatgact gtccgtttta ccacaagcag gaaacagtcc gagcgccgaa
     1501 tcgaggactt gaggttcagg aagcattgta cgacggccgg gatgttttgg ggcataggta
     1561 cgattttgta ctggcaccgc tacccggaga gatctcagcc cgcaagatcg ttctgccgtt
     1621 ccgcagtgat ctggtggata aattggctat tggtaagggt gatctggtag tgggcaggcc
     1681 aatgggagcg ggttgccccg taccacacgt gcttcgtgtg attgaagcca gccccataac
     1741 cgggctgctc accacctggg tggtgggacc ccagtatgcc agggatcacc cggtcaaaga
     1801 ggttgatgcc tatcacatgg tgggttttga aggattggcg gtacaaattg aaaaacagcc
     1861 ggagtttggc tgcagagtga ccttcctgcc gggattctgc atgatgaact tgaatcatac
     1921 cggtttggta aacatgcttt tgaagcaaac cgaaggctac catgtgcgga tcgaggatat
     1981 ccggattctg gcggggatat aggcgtgagg cgagaggcaa cgggcaacgg gcaacgggca
     2041 acgggcaacg ggcaacgggc aacaggcaat agaaaaatga aaagtgaaaa gagaaaattg
     2101 gatttcaccc tccgctcttc aaacttcaca ctttttacac aaggatcggt ctcattcatg
     2161 ggtaccgatt ctatttattt cttcctgcct cggatggatc agagaccgga gaatgctaga
     2221 tttgccccac ccggataatc ttgaaaagag agtgggttta tgctatattt aatgtattca
     2281 aaacctatcg attaactaat aaattatctg gttcgcccat cgatgttgaa ccttgatggg
     2341 tatttatcaa ttatacgttg aaaccaggaa tgagtcgtcg ttaagagagg ttcgggctcg
     2401 aaaggagtac ttatcgtgca agtttccaca cggggcagat acggtttgcg tgccatggtc
     2461 gacatcgccc ttcatataac ggatggtccg atggccttgc gtgtcattgc cgagcgccaa
     2521 gggatctctg aatcctatct ggagcaggtg ttcaccaact tgcgtaaggc tggtttggtc
     2581 aaagcctccc gtggttccca gggtggatat gaactgaacc atcccccgca ggaaataacg
     2641 gtgggacaga ttctgcgttc tctcgaagga ccaatcgttc cggtgttttg cgttgatgaa
     2701 tcaagctccg gcaatatttg tgaacggtcc aagatctgtc cgacccgttc cttttgggag
     2761 gaactccggg ataagattaa cgaattcttg gacggcacca cgttgcaaga tctggcggat
     2821 cgggccagtg cggccatgcc ggaagagccg atgtatttta tctagccggt acacatcctg
     2881 gcgtacggtt cttcggaata ctaccgtcac ttaagcagaa actttgctaa actgaaggct
     2941 tacccgtatg aggtaagcct tttcatttta accggcacaa atcaatccgt tagaaagcga
     3001 cttccggttc atcaggttat tatccgatga tttccgccaa ttggtattcg gaaggaagga
     3061 tcctgccttc cggaatggaa ccgcgtaggt tctcccaaag ggcggcggga tcgtcgggaa
     3121 gcccggccag ctgttcggag agacgttcca ggtaaggttg ctcccgtttg cttaaagcca
     3181 gccggccttc atctactccc tcccgcaaca actgtaacgc ggtggagacc gcctttttgg
     3241 tgcgccgata cggatcggct gctgtcatta attcccggga tacttgaaag acgaaatccg
     3301 gacgcaatac gtaagcctgg ggatcgaaac ggctgtcgga ctccaccatc caatcgcgca
     3361 gcattacggc ggcttcgaat ccttcttgag aggcttgatt catcaaccgg gtgtcataga
     3421 tcagttgttc cattgagacg gtgggagcca ttcctcccag caacttgata ttttgaaccg
     3481 attcattgct ccagagatcg cagattgctc cggcgatatt tcccagaggg ctgagatggg
     3541 cacaggcgct ggatttaccc tccatggcga tgggcactcc ggcaatgact ttcatatagg
     3601 gtccttcgta ggcgcagtct ttactgggcc cgaccgcacc cgcctcatat gcgatcaagc
     3661 tgcgggggac tgtagccacc cggaccgcgg cagcgaagac tttggagata tacccttgtt
     3721 ccgccaggat catggcggta tttccgaacc cgcaggcggt atcccccgag gcaatacaat
     3781 tatatttggt tgcgatggcg acaatctcct gccagaggcg tttcatatca tacgctccca
     3841 ggacgccgag ggcgaaaatg acggaggcga tatccccgtt catcaaagcg tcatcgcata
     3901 tttctttgcc tccggtggat tcaatcgcca agaaatcggc accggccttg gccgattctt
     3961 caaaggttac caacatattt tcccagtata gaccggagta aagcaagggt gggcgtttca
     4021 tctcccgggt atcgttgggc gttacccgta ataaactttt caggccttgt ttcgcttcgt
     4081 aggaaatcaa cgtatctttg actaccttaa ctacatcaag tccccattga gggtggaccg
     4141 tcatcggcgg aagtaattcc acctccacca caatcccggg ggaatggagg accaccgccc
     4201 gttcgcagac gccttggatg atctggcggt attctttgag aatatcagga atggtccccg
     4261 actcaaccgg catcggaggc aaggtgaagt tgatctcggg atagaccgtc cccccgccga
     4321 tgaccatccc gtttctcccg gtaaccggcc ggggtgcccg tccaaaaagc atatcttccg
     4381 gggtatggta cgccagggga ttgataattt gtttgaaagc gttcatcatt cgcaccttcc
     4441 tttccttaat atccaatcta cctcaatcgg ggatgatctt ctataacagg cttacgagtt
     4501 tttgtaattt attattaacc ccatttgttt agtgggattt taacctcgta gaaaaaggag
     4561 acacatacta tctgcggtag gggatttatc tattctacct aagtttggct cggatggaag
     4621 gaatatcgta gcaaattgcg aatacaagca acatgaattt taaaaaaccg cagcacagag
     4681 gagcctcaat gcatattccg gatggttttc tagatgctaa aacctggaga acctcatcgt
     4741 ccaatgtttg atttttcagg atggcggact tttagcgccg agtgcctgtt gcctttgaat
     4801 gaggaggaga atgatggctg attccaagat gcatcgtttc agtcgtaccg aactattgat
     4861 cggcgctgag ggagtagctg ctttgagtcg ggcccgtgtg gcggttattg gacttggcgg
     4921 agccggttct tatgctgtag aggctttggc acgggcggga gtcggttggc tggtgctggc
     4981 agacccccgg atggtggata tttcggatac caaccggcag cttacagcgg caggggatgc
     5041 atatggcaaa ccgaaagttg atgttttagc ggaacgggta aaggcaatta atcccgaagc
     5101 agtggtggtt acctggcagg agtgggccgg cccttggaat ctcgaggcta tcttgcacgg
     5161 caaggttcaa tggatgttgg atacatgcgg tttacccctg caacgtgcgg aacgaatcgg
     5221 ttttgctgcc ggccggggga tcccggtgat ggccatatta gatacaggag gccgtaccga
     5281 tcccctgtcc tttcggaaat cggatatcgg atcgatccgg gattgtcagg aggctgaagc
     5341 tctgagggtc gccttatacc aacggggaat taaaacaggg gtgtcggccg tctattcgac
     5401 tgaaccgccg gttcgacaaa ctacggttgc ggaatccggt tgtcatggat ctgcctgtca
     5461 gaaacgttca tcatgccggg gatgttccgg ttgccgtcag ccggatggga gcctatcgta
     5521 tctcgcgcct atggccggct taattgcagc aggggaactg atccgaacct tgcagacggt
     5581 ttaaggggag aaatttatat cggagtaatt taggccacat tcggggagac ccggatgtgg
     5641 cctgtttaat tcatccctac catgaatgct gccctctcct cgctatggca ggaatcgata
     5701 tatgtaaagt atatgttagc ttttgatgtg tacattgtat ttcttacgtt aataacttga
     5761 tatgacgtca tatttatgtt agaatttgca caacaaagct ttcgaccggt tcaataatcg
     5821 acttgaatat tacaaaaaca ttacattgcg gaggtgatat cctgtccggc tttggccggg
     5881 gccggatccg gcaagggttt atcaggttgt ctttcgtatt aagtgaattt ttcggacaaa
     5941 taacttaagg agtgattcgg atgagtgatt ttttgcggaa atccccctct gtcaagattg
     6001 gcatcggact ggttttaagt ttactgttgg tggccatggc ggcatcggtg accatggcgg
     6061 ctcctgatcc gacgggagcg gctacattac aaaaagaccc caacgcaccg gtcaatttta
     6121 tctggacttt gatctgcggg ttccttgtaa tattcatgat gaccgggttc gcgatggtgg
     6181 aaaccggttt tactagagcc atccacgcaa acaacacgat gttgatgaac atgatgattt
     6241 gggtatttgg aaccctcggt tatttcctag tcggttttgg tctgatgttt ggcggtgtcg
     6301 gtggagttta cgagggttta tccaaggcgc tctcgattaa tatcggcggc catacatgga
     6361 acctcttggg attatccgga tttgccctgg gaggcaaggc gtacgatgtc ggtattttca
     6421 tgctcttcct attccaggcc gccttcatgg acaccacggc taccatacct acgggcgcaa
     6481 tggcggaacg gtttaaatgg agcgccttca ttgtatacgg cttcctggta ggcgggatca
     6541 tttatccgat cttcggcaac tgggcctggg gcggaggatg gttgtcacag ctcaacgtcc
     6601 ttggcttggg agcgggttac aaagatttcg ccggatcggg tgtagttcac gcagtgggcg
     6661 gtctgacggc tttggccgga acgatcatca tgggaccgcg gatcggcaaa tttgtgaaag
     6721 gaaaaccggt ggccatgccg ggccatgacc tcagtatggg cgtgattgga acctgcatcc
     6781 tgatcttctg ctggttcggt ttcaacgccg ggagtaccat ggctgccacg gatctccgtt
     6841 tgactgtggc cgcagtcaat acaatgctcg ccggatgtgc cggcggtttg accgccatgt
     6901 tatatatgat gaaatctacc ggcaaaccgg acaccagtat gtcctgtaac ggagtgctcg
     6961 ccggtttggt ggcgattacc gctccctgcg cctacgttcc ggcatgggcg gcggtagtaa
     7021 tcggcggtat cgccggtgta ctggtttgcg cttcggtctg gtttgtggag aatgtactga
     7081 agctcgatga ccctgttgga gcggtttcgg ttcacggcgt taacggaatg tggggtgtgc
     7141 tctcggtggg gctgtttgcg gacgggacat acggtggcgt taaaggactg ctcttaaacg
     7201 gtgactgggg tcagctgggg gcgcaagtgg ttgcgatcct ggccttgata ttattgatct
     7261 tcccctttgc cttcttggtg ttgaagttaa tcgacaagac cattgggttg cgggtctcgg
     7321 ccaaggagga actggccgga ctggatatgc cggtacacgg tgccctctgt taccctgagt
     7381 tcgctctaag cgccttggga accgcccaaa ctttccctga ggaagattct caggcatcgc
     7441 ggacagctgt taagtctgcc gcagtatcaa aggaggctta aagaaatgat taaaatagaa
     7501 gccatttttc gcccgagccg ttttgaggcc gtgaaggacg ccttggccaa ggtggggatt
     7561 cgcggcatga cggttagcga ggtcaagggt tgcggcaaac aaaaaggcgc agttgagcac
     7621 taccggggtt cggagtacga gatcgttctc catcccaagg taaagatcga gattgtcact
     7681 ttggaagaga atatggagaa gatcatcaca gcgattcaat atgcggcgca aaccggggag
     7741 accggggacg gtaaaatctt tatttaccag ttggctgagg cgattcaggt tagaactggt
     7801 gaacgtggca agaacgtcat ctaaaagttt taaaacatgg aggctatctt ggaataatgg
     7861 gatagcctct tgtgatctga gggactcccc tcgtatcgga tgatttaaca agaagacttc
     7921 agggagtaaa cgtggagagg aataataggt aatggattca tgatgattga tggaaggaaa
     7981 aacagttttt taaacgaatt acttctgtta attatccgac tgctccattt acatgattgt
     8041 tacaggaaat agtcggacgg ataatccgga tttttgagga catttat
//
LOCUS       DASVMB010000503        12361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_208019,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000503 DASVMB010000000
VERSION     DASVMB010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208019"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VF531_14075"
     CDS             <1..1271
                     /locus_tag="VF531_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7715138.1"
                     /translation="KIGTHVKQYFNQQTLFTAITGASELCISPDAALPVLNLLIAKAL
                     SERVPVHLAIPQDILAAEINGNIAVPLPIPPHSEGKGFFSGDLEKALTWMNEAQRPVF
                     IIGRESRPYTTPLRSLAAGYGAGIVIGLDNKGGLPDTDPLVIGGVGPANLPESWSEID
                     LIISFGEAVYERPYLPEGARYIRLCDETSVLYDDGALLLNGDLETLINRLAEKSIVPG
                     VREAWRARIETTRRNNQIQLRERSTPRDPVRFFRVLASLIRPDALITLDVGEFMHWFN
                     WSFPAQTQTVLVSSNWRSMGCALAAAIAAKLHHTERQVVAIIGDGGFLMSLGELNTVI
                     RLGLPLTVLVLRNGVYGLEMQKMAQMGFKPCGTELQLPNIPQLASACGWNSYQLNDLN
                     GCESILETAFQNPASLVDVNIDSQKLPYLK"
     gene            complement(1643..3727)
                     /locus_tag="VF531_14080"
     CDS             complement(1643..3727)
                     /locus_tag="VF531_14080"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX7715139.1"
                     /translation="MKRLLVRITLICVLVLVIFVVTLWGKSPEKERILPQLLMESLGN
                     WHYDPVPVDDRFSKRAYTLLIKSYDYEKRFFLESDLAELQKYQNEIDDQVKQGTTDLL
                     SRSEAIFRERISEVQAWADEILKQPFDLKATESLETDPAKLSYCKNSAELKERWRKIL
                     EYQTILRYLELIDSKDAGKAKSGSLGPIQPQLEQEARHDVAVSIKYTLGRMLKEDSSA
                     SLERYLNALAGSFDPHTEYFSPTKKEDFDISMTGKLEGIGAMLGQDGAYIKVVEIVPG
                     SAAWRQQELKPEDIILKVAQGQEEPVDVTNMSADDAVKLIRGPKGTEVRLTVKKPDGR
                     IMVIPIIRDVVVVEETYARSAVLVNKKTGKKTGYINLASFYHDFQNPRGRNSATDVRK
                     ELEKLKAAKVGGVILDLRNNGGGALEDAVQMAGLFIKSGPIVQVKSGSGRGEVYNDPD
                     GGVVYSGPLVVLVNSYSASASEILAAALQDYHRAVVVGSNSFGKGTVQTIIDLDQLLG
                     NQMADLKPAGSLKITVQKFYRITGGSTQEKGVTADIQLPEVYSIPELGERSLDYSLPW
                     DTVKSLRYSEWNQEPKIVALRQQSAARIKVDPAFRLIVNDQAKFQKISTTTLQPLNID
                     KIRASQQLIASESARLDQLEVKLNNIKASFPGKTGITTDSKLKDWLTKVEKDCYIGEA
                     TFILNDIDRGKR"
     gene            complement(3997..4746)
                     /locus_tag="VF531_14085"
     CDS             complement(3997..4746)
                     /locus_tag="VF531_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7715140.1"
                     /translation="MFEVWFYSLGSVIAVSALSLLAAIFLVVGEHRLQKVLLFLVSFA
                     AGGLFGDALIHLLPESFEKLGAKLSTSLWVIAGIILFFVLEKFIHWRHCHDLNCEGHA
                     HPMVAVNLVGDGVHNLIDGMLIAASFSVSRPLGITTTLAVVLHEIPHEIGNFGVLVHG
                     GLPVRRALFFNFLTALTAIGGAILALLIGPRLEGFTLALLPVTAGGFLYVAGSDLIPE
                     LKHEVELSHSVLQLISMSCGVGLMALLILVE"
     gene            complement(4872..5276)
                     /locus_tag="VF531_14090"
     CDS             complement(4872..5276)
                     /locus_tag="VF531_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7715141.1"
                     /translation="METIDLHGYNQQEARELLERRLQNAAAYGERVVRIVHGQGKHSE
                     VFPVLKSFVRRWLEESDLAAMLVAEVYRGEDGSPYTLPNAGETILVLKGAGTTYHEPQ
                     LNPDDEEEYEARRQAKAIMADRLRVARRRRRR"
     gene            complement(5672..6019)
                     /locus_tag="VF531_14095"
     CDS             complement(5672..6019)
                     /locus_tag="VF531_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715142.1"
                     /translation="MTGAVLGFWLFLILLRIFFGALGRATVTLPALLIISVVCMVPLG
                     LYVYMAWRHLLIGASLGLGAGIVAMLWLNIRGGGWEDLSGVSVLMEAAAFGTVIGIIV
                     ECGYKLYQRFRRE"
     gene            complement(6073..6414)
                     /locus_tag="VF531_14100"
     CDS             complement(6073..6414)
                     /locus_tag="VF531_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715143.1"
                     /translation="MEEVQPRIFENREFVEFEEEDIRRLNRMEAEKFDRNPELRQVME
                     TITAEVGERGRWEEQWVTVDHSGRRVYARIYRGPDRVVAVTADGRIVRDFDYSPRSEP
                     GPQSIDEPSNW"
     gene            complement(6534..7124)
                     /locus_tag="VF531_14105"
     CDS             complement(6534..7124)
                     /locus_tag="VF531_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEX7715144.1"
                     /translation="MIKTLFSNPHYFSLIVLPILIFLARICDVTIGTIRIIFVSRGRK
                     QLASFCGFFEVLIWLAAIGQIMQHLDNVWCYLAYASGFAMGNFIGITLEAKLAMGILV
                     VRIFTQNDGSRLITRLKQAGYGVTCLDGQGTTGPVQVIYTVIQGSSLGIVDRLIRDYN
                     PKTFYSIEDVRFVSAGVFPVQHHTFSPFRFPGRKRS"
     regulatory      7267..7425
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            7681..8535
                     /gene="dapB"
                     /locus_tag="VF531_14110"
     CDS             7681..8535
                     /gene="dapB"
                     /locus_tag="VF531_14110"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7715145.1"
                     /translation="MIKTCLLGLGRTGMVVADAILKAPQFELVSVISKPGGNKIGRPL
                     SEYLPGAGELIIQDSQDLESEIETTKFTVAIDFTSPAAALQNTKTLAKHGVNVVIGTT
                     GFNNIQLFEIEKITEQAKIGLVYAPNISVGINLLLSLVKTIARMIPHYDVDITESCHR
                     HKQDAPSGTALRIAEAIYKAKGEPRSPRLRRDQKGLQLREKGEIGMHSIRAGGIVGVH
                     QVLFAGESDEIEITHRAYSRTVYADGALKASTFINGRRGFYHMEDVLGTPEGSASGIL
                     DRIRIHCN"
     gene            complement(8766..9317)
                     /locus_tag="VF531_14115"
     CDS             complement(8766..9317)
                     /locus_tag="VF531_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7715146.1"
                     /translation="MYCIRCGAELPEMAAYCPKCRQTVGELPVGCNFAGFWKRLAASL
                     LDGAILGIVQSFLAIIGGMILGFSFEMWVAEESNGFFQMVPVIGSVFAGLGLVSLVAT
                     WLYYALFESSELQATPGKLVLGILVTDEAGNRINFGRASARYFGKIISALILMIGYMM
                     AGFTEKKQALHDIIAATLVVNKR"
     gene            complement(9475..10956)
                     /locus_tag="VF531_14120"
     CDS             complement(9475..10956)
                     /locus_tag="VF531_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HEX7715147.1"
                     /translation="MRILLVYPQYPDTFWSFKHALRFISKKASNPPLGLLTVAALLPR
                     EWEQRLADLNVRPLGDNDLLWADYVLISAMTVQRASARQIIDRCRNLGVKTVAGGPLF
                     TTCPEEFPDVNHLVLDEAEVTLGMFLADLQAGSPKPVYTSKIRADITQTPPPRWDLVD
                     CSKYASLSLQYSRGCPFNCDFCNITMLYGHQPRTKELGQVLAELDAIYALGWRGSVFM
                     VDDNFIGNKQKLKQEILPALIDWMARRRHPFNFYTQVSINLADDEVLMELMVRAGFDT
                     VFVGIETPNEASLAECSKVQNKQRDLVAAVKKIQHQGLQVQGGFIVGFDSDPQSIFVN
                     QIEFIQKSGIVTAMVGLLSALRGTGLYERLQKEKRILKEGTGNNTDGTLNFIPKMPPA
                     TLLSGYRQILATIYEPEFYYRRIREFLREYRPRPVRIRRLHLSDIEAFFGSILILGII
                     GKERAHYWKLLGWSLVKNPRAFPVAVQYAIYGFHFRKVLAETV"
     gene            complement(11136..11918)
                     /locus_tag="VF531_14125"
     CDS             complement(11136..11918)
                     /locus_tag="VF531_14125"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEX7715148.1"
                     /translation="MSEQGVTRFAKQFYESVTRIESYRDRLRGQLGEAIVFLVIVTLS
                     LGTLSMIRPILEFNWGIRQAAIEIERDLPDFILRDGQLTAAKPFLMRKGKQSIVIMDT
                     SGVHRPEEMEGYMEGVFIGKDRIILQQNGFTRREMGFNEFAGVEFTKTDLVRFLPYLQ
                     WLDPFIFIFGWFGFFIGKLCSAIILSLLGLIIAARLQTRMGFKGLYKLSIYALSLPIA
                     LKVLLALTGLTIPFFWLIYYGAALTYLGLALSEIKQANDLIG"
     gene            12123..>12361
                     /locus_tag="VF531_14130"
     CDS             12123..>12361
                     /locus_tag="VF531_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715149.1"
                     /translation="MDIGKSLQTSLDLYLKHFGILFLATLIGAIITAVSFGILAGPVC
                     GGLLLLGLNLLRGSTGEVREVFQHFDKFLPTLLVTL"
BASE COUNT         3184 a         3387 c         2861 g         2929 t
ORIGIN      
        1 ccaagatagg aactcacgtc aaacagtact tcaatcaaca gacccttttt accgcgatca
       61 ccggcgcctc ggagctttgc atatctccgg atgcagcgct ccctgtactc aacctgctga
      121 ttgccaaggc attatccgaa cgcgtcccgg tccatctggc gattccccaa gacatcctgg
      181 cagccgaaat caacgggaac attgcggttc cattgcctat tccaccccat tccgagggta
      241 aagggttttt tagtggcgat ttggaaaaag cacttacatg gatgaatgaa gcccaaaggc
      301 cggtattcat tatcggacgg gagtcacggc catatacgac cccattgcgt tccctggcag
      361 ccggttacgg ggctggaatc gtgatcggat tggataacaa gggtgggctc cccgataccg
      421 atccgctcgt tatcggggga gtcggaccgg caaatcttcc ggagtcttgg tccgaaattg
      481 atctgatcat ctcatttgga gaagcggttt atgaaaggcc atacctaccc gaaggagcac
      541 gctatattcg tctctgtgat gaaacatccg tgctttacga cgacggcgca ttgttattaa
      601 acggcgatct ggaaacccta atcaaccgat tggcggaaaa atcaattgtt ccgggagtca
      661 gggaagcatg gcgggcccgg attgagacaa cccgccggaa taatcaaatt cagttgcggg
      721 agaggagcac cccacgggat ccggtgcgat ttttcagggt tctggcttcc ttgatccggc
      781 ccgatgccct tataaccctt gatgtgggag aatttatgca ttggtttaat tggagtttcc
      841 cggcccaaac tcaaacggta cttgtctcat ccaactggcg cagcatgggg tgtgccttgg
      901 cagcggcgat tgcagccaag ctacaccata ccgagcgcca ggtagtggcg atcattgggg
      961 atggtggttt cttgatgtcc ctgggggaac tgaacaccgt aatccggctc ggacttcccc
     1021 tcaccgtact ggtcctgcga aacggggttt acggcctgga aatgcagaaa atggctcaga
     1081 tgggattcaa accctgcgga accgaactgc agttaccgaa tataccgcaa ctcgcctccg
     1141 cctgcggctg gaatagttat caactgaatg atctgaacgg ttgcgagtca attctcgaga
     1201 ctgcctttca aaacccggca tcattggttg atgtcaatat cgactctcaa aaactgccct
     1261 atttaaaata aggttagcat atgaccgaac cacgatcccc ttctctcccg gcaagcagga
     1321 aacaatagac ctcggaggat agtggttact ctctctggcg cttgtcgcca gagagagcaa
     1381 gagtgagtcg taggcggtat tgacgactca cccctctagc tacccttcac tcccggcaag
     1441 tgggaaagag gggagaaggc ggattcaagc agtaaaaagc ttcgaaggat cgaggtgacg
     1501 cgcgcttgtg cgtgcgaact ctctctggcg cttgtcgcca gagagagcaa gagtgagtcg
     1561 taggtagtgg gaaaaagcaa taaacgataa cagggccggc aaatggccgg ccctgctaat
     1621 ataatgactt ttcaaaatcc ttttaacgtt tcccgcggtc gatgtcgttc aagatgaagg
     1681 tggcttcacc gatatagcag tccttttcga ccttggtcag ccaatccttg agtttcgaat
     1741 ccgtggttat ccccgttttc ccggggaacg aagccttaat attgttcaac ttgacctcca
     1801 actgatccag ccgggcgctt tccgaggcta tcagttgctg agaggcacgt atcttatcga
     1861 tattcaacgg ctgcaatgtg gtggtgctga tcttttgaaa tttggcctgg tcattcacaa
     1921 tgaggcggaa tgccgggtca accttgatcc tcgccgcact ctgttgccgc agggcgacga
     1981 tcttcggttc ctggttccat tcgctatacc gcaagctctt caccgtatcc caaggcaaag
     2041 agtaatcaag cgaacgttct ccaagctcag gaatgctata gacttccggc aattgaatat
     2101 ccgcggttac ccccttctcc tgagtggaac cgccggtaat cctgtaaaac ttctgaacag
     2161 tgatcttgag agagccggca ggtttcaagt ccgccatttg attaccgagc aattgatcca
     2221 gatcgataat ggtttgaacc gttcccttac caaaggaatt actgccgacg acgaccgccc
     2281 ggtgataatc ctgaagtgct gcggctaaaa tctcagacgc cgaggcgctg tatgagttga
     2341 ccagcaccac cagcggaccg gaataaacca cgccgccgtc cggatcatta taaacctcac
     2401 cccgtcccga accgctcttc acctggacga taggcccgga tttaatgaac aaaccggcca
     2461 tctgaacggc atcctccaag gcacctccgc cattattccg caaatcaaga atgaccccgc
     2521 cgactttggc ggccttcaac ttctccaatt ctttccggac atccgtcgcc gaattacggc
     2581 cgcggggatt ttggaaatcg tgataaaacg aggccaggtt gatatagccg gtcttcttac
     2641 cggttttttt attgacaagt actgccgaac gggcatacgt ctcctcgacc accactacat
     2701 cacggataat gggaatcacc atgatccgac cgtccggttt cttaacagtc aagcgtacct
     2761 cggttccctt aggaccccgg atcagtttca ccgcgtcatc cgcagacatg ttggtcacat
     2821 ctaccggttc ctcttgaccc tgcgccacct tgaggatgat gtcttccggt ttcaactctt
     2881 gttgccgcca agcggcgctt ccgggcacaa tctccacaac cttgatatag gcaccgtcct
     2941 gacccagcat ggccccaatt ccctcaagct ttccggtcat tgagatatcg aaatcctctt
     3001 tcttggttgg agaaaaatac tccgtatgcg ggtcgaaact tccggccagg gcattcaaat
     3061 atcgctccag ggatgcgctt gagtcttcct taagcatccg gcccagagta tatttgatac
     3121 ttaccgcgac atcatggcgc gcctcctgct ccaactgggg ctggattggt ccgagcgaac
     3181 cggatttagc ttttcccgca tcctttgaat caatcaactc caaatagcga aggatcgtct
     3241 gatattcaag tattttgcgc cagcgctctt tgagttcggc actgttctta cagtaggata
     3301 gtttggccgg gtcggtttcc agactctccg tggcctttaa gtcgaaaggc tgttttaaaa
     3361 tttcatcggc ccaagcttga acttccgaga ttcgctcacg gaagatcgcc tcggaacggc
     3421 tcagaagatc ggtggtaccc tgtttcacct gatcatcgat ttcattttga tatttttgaa
     3481 gctcggccag atcggattcg aggaaaaacc gtttttcata atcgtaactc ttgatcaata
     3541 aagtataggc ccgtttcgaa aaccggtcat caactggaac cgggtcataa tgccaatttc
     3601 ccagactctc cattagcagc tgcggtaaga ttcgttcctt ctccgggctt ttaccccaaa
     3661 gggttaccac gaagataacc aaaaccaaaa cgcagatcaa cgtgattcga accaataatc
     3721 gtttcatcct atttttcctt tgatttaaaa tttaaagagc ctgataagca tgttacttat
     3781 cattttacca tgaatgtcaa tccgggtcat gccttacgac accggtcgac agaggagttt
     3841 tcaccgcttg aacttgtttt tatggaaatc gagtcaatcc aagcatccgt tggtcggata
     3901 tccaaaatca accacccggc ccgaatgcag aagaaatgca ggatgggccc agcaaatcaa
     3961 gaggctcggc aatcaaaaag ctttaaccgc gtactgctat tcaacgagta tcaacaacgc
     4021 catcaagccg accccgcaac tcatcgagat gagttgcaat accgaatggg acagctccac
     4081 ctcatgtttg agttcaggga tgagatcgga tccggccacg taaagaaaac cgccggctgt
     4141 aaccggtagc agagcaagtg tgaaaccctc caaacgcggc ccgatgagta aagcgagaat
     4201 cgcaccgcca atagcggtga gcgcagtcaa aaaattgaaa aataatgccc gcctcaccgg
     4261 gagaccgcca tgaaccagca cgccgaagtt accgatttca tgaggtatct catgtaacac
     4321 aactgcaagc gtggttgtga ttcctaaagg ccggctcaca gagaaacttg cggcaatcaa
     4381 cattccgtcg atgaggttat gaaccccatc accgacaaga ttcaccgcca ccatgggatg
     4441 ggcatgcccc tcacagttca gatcgtgaca atgccgccaa tggatgaatt tctccagcac
     4501 gaagaagaga atgattcctg caatcaccca gagcgaggtg gatagtttcg cacccagctt
     4561 ttcaaaggat tccggtaaaa gatggatcaa agcatcaccg aaaagcccgc ctgcggcaaa
     4621 actgactaaa aaaagcaata ccttttgtag cctgtgttct ccgactacca agaaaatcgc
     4681 agccagtaga gaaagtgcgc ttaccgcaat cacgctgcct aaactataaa accaaacctc
     4741 aaacataata aatcgcctca ttccccgagc tttccggaaa aatttactcg gcaggtttta
     4801 tctgaacgtt tcaatatgcc gaatcatgga taatcaatta gatccgatag tggcccgcat
     4861 tacgcctttc ctcaacggcg cctccggcgc cgcgccaccc ttagccgatc agccatgatt
     4921 gctttggcct gacggcgggc ctcatattcc tcttcgtcgt ccgggttcag ctgcggctca
     4981 tgataggtgg ttccggcgcc tttcaagacc aaaatagtct caccggcatt cggcaaggta
     5041 taaggtgagc catcttcacc acggtagact tcggctacca gcatcgctgc cagatcggat
     5101 tcctcaagcc aacgacggac aaatgacttt aaaaccggaa aaacctcgct atgcttcccc
     5161 tgaccgtgaa cgatccggac aacccgctcg ccatatgccg cagcgttctg tagccggcgt
     5221 tccaacaatt cccgggcttc ttgctgatta taaccatgta aatcgatagt ttccaaaaag
     5281 ctctccaccc atccattgtt gcctaacgcc tcaagaacct gtgaccggac agagccttgg
     5341 attcagtttc cagtttatac tcttccacaa ccggaccgtt tttcctgcct aagccgggaa
     5401 ctgtccccca aaaccgtttt gtcgttggtt ccttgattca acttgtcatg attttttcgg
     5461 aaacccgacg ggtgccggac aaaacaaaac cgcccctttg agcggctgtc cgattattcg
     5521 attccgtacc ctatccgcaa cctgatgtac atctgtttaa acgcaatttc actctcaaat
     5581 gttcccgtct tatgagttac tatcatattg tgttccaaat tacattccct tatacgatta
     5641 cgaaacggca tatttcgggg acaacaccga atcactcccg ccggaacctt tggtacaact
     5701 tgtaaccgca ttcaacgatg atcccgatca ccgtgccgaa ggcagccgcc tccatcaaga
     5761 ccgaaactcc ggataaatcc tcccaaccgc cgccccggat attcagccac agcatggcga
     5821 cgatccccgc ccccaaaccc aaactcgctc cgatcagaag gtgtctccag gccatataga
     5881 catataagcc cagcggcacc atacagacta cggagataat caacaacgcc ggaagagtga
     5941 cggtcgccct tcccagcgcc ccaaagaata tccgaagtag aattaagaat aaccaaaatc
     6001 ccaatacggc tccggtcatc ggcgcccacc aacgccggcg gtatttgctg aaccaatacg
     6061 tcaagcaacg atttaccaat tgcttggttc atcgatggac tgtggacccg gttccgaccg
     6121 gggggaataa tcaaagtccc ggacgatccg gccatcggcg gttaccgcca ccacccggtc
     6181 cgggccgcgg tatatccggg catagacccg cctaccgcta tgatcgacag ttacccattg
     6241 ttcttcccag cggccccgtt caccgacttc ggcggtgatc gtctccatga cctgacgtaa
     6301 ctcggggttt cgatcaaact tctcggcctc catccggttt agacgccgta tatcctcctc
     6361 ctcaaattcg acgaattccc ggttttcaaa gatccgcggt tgcacttctt ccatttttcc
     6421 gcctcctaaa aaatacccgc tatccggatt tattttatcc gggttgggac gggctaaacg
     6481 atggatttgt taaaaaaccg gatacggatt ttattataat atgaggccgc ctactaggaa
     6541 cgtttccggc cgggaaaccg gaacggacta aaggtatggt gttgaaccgg aaacacaccg
     6601 gcactgacaa aacgcacatc ctcaatactg taaaaagttt ttggattata atcccggatg
     6661 agccggtcca ctattccgag cgagcttcct tgaatcacgg tataaatcac ctgtaccgga
     6721 ccggtcgtac cctgcccgtc cagacaggtt acaccgtatc cagcctgttt gagccgggta
     6781 attaacctgc ttccatcgtt ctgagtgaat atccggacca ctaaaattcc catggccaac
     6841 ttggcttcga gcgtgattcc gataaaattc cccatggcaa aaccactggc ataagcgaga
     6901 taacaccaga cgttatcaag atgctgcata atctgtccga tggctgccag ccagatcaag
     6961 acctcaaaaa agccgcaaaa cgaagccaac tgctttctgc ccctggatac aaagataatc
     7021 cggatggtgc cgatggttac atcacagatc cgggccagga atatcaggat gggaagcaca
     7081 atcaatgaga aataatgggg attggaaaag agtgttttta tcatggcgcc tcaagaatat
     7141 tctccgctat accgtcagta acttataccc tccccaacct ctttgaaatt atccagcaat
     7201 ccccatgccc agaccgcttg actatatttt ataaaactgt ataataatag taatcttata
     7261 gcccataaca catgaacgct taatctcact tttggtgaga acgggggaac caatttttcg
     7321 gggtgaatga atatgctcgg atttagatca tccgagacaa ttttagggtc actcttacga
     7381 cccgaacccg gcagctaact ccgtaggcgt ggaaagaggg gtgaacatga ggccgatatg
     7441 tctccatgga cttcctccat ggtttttttg tctcctgagc ctgattcatt ttcctagcag
     7501 ttaaacccaa aatcatcgct catacaactt ctgtaaagga ttcgttttac agatttcata
     7561 acggaaaccc actcaaaaaa gagccggtac ttaagatacc ttttaaaaaa tcaaataact
     7621 acgaatgagg aacgatcgct tcatccgaat tgtaaaaaca tagtatagag gtgttacatc
     7681 atgataaaga catgcctgtt aggactcggc cggaccggga tggtggtggc cgacgcaata
     7741 ttaaaggccc cccaattcga actggtctcg gtcatcagca aacccggcgg taacaaaatc
     7801 ggccgcccgc tgtccgagta tcttcccggt gccggagaac ttatcattca agactctcag
     7861 gaccttgaat cggagattga gaccacaaag tttaccgtag ccatcgattt cacctcaccg
     7921 gccgcagccc tgcaaaacac caaaaccttg gccaagcacg gagtgaacgt ggtgatcgga
     7981 accaccggat tcaacaatat tcagcttttt gagattgaaa aaataaccga acaggcaaag
     8041 attgggctgg tctacgctcc caatatctcc gtaggcatca atttacttct ctcactcgta
     8101 aaaaccatcg cccggatgat tccccattat gatgtggata tcaccgagtc ctgccatcgt
     8161 cataaacagg atgctccatc cggaaccgca ctccgaatcg ccgaagcgat atataaagcc
     8221 aagggggagc cccgaagccc gagactgcgg cgtgatcaga agggtttaca actgcgggag
     8281 aaaggtgaaa tcggtatgca ttccatccgg gccggaggga tcgtgggagt ccatcaagta
     8341 ctttttgccg gagaatccga cgaaatcgag attactcacc gtgcttactc ccgtacggtt
     8401 tacgccgacg gcgccttgaa agcgtcgacg ttcatcaatg ggcggcgtgg tttctatcat
     8461 atggaagacg tcctggggac cccggaggga tcggcttccg gaatcctaga ccggatccgg
     8521 atccattgta actaatcggt gcaccagact tacatctcta caatcggtca ctgagagatt
     8581 atccacagaa ttcaagaatc tatgtccatt ttgttattct cccgcagagg cgtcggcacc
     8641 aaaaggtgcc gcaggcgcag agtacaaaga aaaactcaac gcctcttgcg ccttggcggg
     8701 agatattctt tttggcgtta atgccttact ggacaaatat ttttaccagc tattaagaac
     8761 atgccttatc ttttattaac caccagcgtg gcggcgatga tgtcgtgcag cgcctgtttc
     8821 ttttcggtga agcccgccat catgtaccca atcatcagga tcaatgccga tataatcttc
     8881 ccgaaatatc gtgcgctggc ccggccgaaa ttgattcggt ttccggcttc atccgtgacc
     8941 aatattccca agacaagttt tcccggggtc gcttgcaact cggagctttc aaacaaggcg
     9001 taatagagcc aggttgccac caagctcacc aatcccaacc ccgcaaaaac cgaaccgatt
     9061 accggaacca tctgaaaaaa accattgctc tcctctgcaa cccacatttc aaaggaaaaa
     9121 ccgagaatca ttccgcctat aatagctagg aagctctgga caatccccag aatggccccg
     9181 tccaaaagac ttgcggccaa acgcttccaa aacccggcga aattgcaacc caccgggagc
     9241 tctcccactg tctgacggca tttcgggcag taagccgcca tttcaggaag ctccgccccg
     9301 caacgtatac aatacatcgc catcccccct tcaagattcg atgatcaata tacgctcccc
     9361 accggatttt tcctttttat cgggtccgtc ataaccgaac tttctagata tcaacccacc
     9421 ttccggatga tcaaatgggt atcaaaagtt tgcagtgaga gacatccccg gtggctaaac
     9481 cgtctccgcc aataccttac ggaaatgaaa cccgtaaatc gcatactgca ccgccaccgg
     9541 aaaagcgcgg gggttcttta ccaaggacca tcctaatagc ttccaataat gagcccgttc
     9601 cttcccgata atccccagga tcagtatgga cccgaaaaaa gcttcgatat cggagagatg
     9661 caaccggcgg attcgtaccg gcctcggccg gtactcgcgg agaaactccc ggatcctgcg
     9721 atagtaaaac tccggctcat aaatggtggc caggatctgc cggtagcccg aaagcagcgt
     9781 agcggggggc atcttcggaa tgaaattaag cgtgccgtcg gtgttgttcc cggttccctc
     9841 cttcaggatc cgcttctctt tctggagccg ttcatagagg ccggtgccgc ggagggcgct
     9901 taacagcccc accatggccg taacgatgcc gcttttctgg ataaactcga tctgatttac
     9961 gaagatggat tgaggatcgc tgtcaaaccc aacaatgaag cccccttgaa cttgtaatcc
    10021 ctgatgttgg atctttttga ccgccgccac caggtcccgc tgtttattct gaaccttgct
    10081 gcactcggcg agacttgcct cattgggggt ttcgattccc acaaaaaccg tatcgaatcc
    10141 tgcgcgaacc atcagttcca ttaaaacttc atcatcagcc aggttgatcg atacctgtgt
    10201 ataaaagttg aacggatgac gacgccgggc catccaatcg attaaggccg gcaggatctc
    10261 ctgtttcagc ttctgtttat tcccgatgaa attgtcgtct accatgaaga cgctgccccg
    10321 ccaacccagg gcataaatgg cgtcaagctc ggccagaact tggccgagtt ccttggttcg
    10381 gggctgatgg ccatagagca tggttatgtt acaaaagtca caattgaaag ggcaccctct
    10441 ggagtactgc agactgagtg aggcatattt ggagcagtcc accagatccc aacggggagg
    10501 cggggtttgg gtgatgtctg cccggatttt cgaggtatat accggcttgg gggagcctgc
    10561 ctgtaaatcg gcgagaaaca ttcccaacgt tacctccgcc tcatccagca ccagatgatt
    10621 tacatccggg aattcttcag gacaggtggt gaagagagga cctccggcga cggtcttcac
    10681 ccccaggtta cggcaacgat cgatgatctg acgggctgat gcccgttgga ccgtcatggc
    10741 gctgattaat acataatcgg cccaaagcag atcgttatcc cccaggggac ggacgttaag
    10801 atccgccagg cgctgctccc attcccttgg aagcagggct gccactgtca ataaaccaag
    10861 gggtggattg ctggccttct tggagatgaa acgtaaagcg tgtttaaaac tccaaaacgt
    10921 atcgggatat tggggataaa ccagtaagat ccgcattcga tgactccttt cggcatgaaa
    10981 ggtatgtagt cgcttgccta cctataaagt atcagccgaa acgtagaggt ttatccccgt
    11041 ttttgaagag ttattccgta atggtcaaga tttttttgtg ctgcacgcga gcaccctcat
    11101 cccgggtttt aaagcattcc cgggccacca ccctttcacc cgatcagatc attggcttgc
    11161 ttaatttccg aaagcgccag accaagatag gtcaaggcgg caccataata aatcagccag
    11221 aaaaacggaa tggtaagtcc ggtaagggcg agtaagacct tgagtgctat cggcaaactc
    11281 aaagcgtaaa tacttaattt gtaaagacct ttaaagccca tccgggtttg gagccgggcc
    11341 gcaatgatga gtcccaggag actgaggatg atggccgagc atagtttacc gataaaaaaa
    11401 ccaaaccatc cgaaaatgaa aatgaaagga tcgagccatt gcaggtaggg taaaaaccgg
    11461 acaagatccg tcttggtgaa ctcgactccg gcaaactcat tgaatcccat ctccctacgg
    11521 gtgaacccgt tttgctggag gatgatgcgg tccttgccga taaaaacgcc ctccatgtag
    11581 ccttccatct cctccggccg gtgaaccccg ctggtatcca tgatgacgat gctttgtttc
    11641 cccttcctca tcaggaacgg tttggcggca gtgagttgcc cgtctctcag gatgaaatca
    11701 ggaagatccc tctctatttc aattgcagcc tgccgtatac cccagttgaa ttccagtatc
    11761 ggccggatca tgctgagcgt ccccaaactg agggtgacta tcactaggaa aacaatggcc
    11821 tcaccgagct gtccacggag ccgatcccga tagctctcga tccgtgtgac gctttcataa
    11881 aactgtttag caaaccgggt cacgccttgt tcactcattt atcgataccc ccttggtttc
    11941 taataccaga tagttaattt ttcatcatgt tttcggcccg gtacctttga actcctgccg
    12001 gttcagtaaa aaaccgaccc ttcactccca atcataaatt tactatggcc ggaatatctg
    12061 atatccgggt agatctttcc cgggacgccc cttcccggaa agaaagaaag gagctgtaaa
    12121 gcgtggatat tgggaaaagc cttcaaacca gcctggacct ttatctaaaa catttcggga
    12181 tcctcttcct ggccactctc atcggggcga taatcactgc ggtcagtttc ggaatcttag
    12241 ccggccccgt ctgcggcggt ctgttattac ttggtctaaa tttgttgagg ggatctaccg
    12301 gagaagtgag ggaggtcttt caacacttcg acaagtttct ccccacttta ctggtaacgc
    12361 t
//
LOCUS       DASVMB010000504         7429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_502055,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000504 DASVMB010000000
VERSION     DASVMB010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502055"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF531_14135"
     CDS             complement(<1..491)
                     /locus_tag="VF531_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX7715150.1"
                     /translation="MFQSKLFAPTLRETPAEAEIVSHKLMLRAGFIRKSATGIYTYLP
                     LAQRVLRKISQIVREEMDREGGQELALPIVQPAEIWQESGRWEVYGDEMFRLKDRHNR
                     EFCLGPTHEELITTLVKNEVRSYRDLPLRLYQIQNKYRDEIRPRFGLMRGREFVMKDL
                     YSF"
     gene            complement(506..1564)
                     /gene="ispG"
                     /locus_tag="VF531_14140"
                     /gene_synonym="gcpE"
     CDS             complement(506..1564)
                     /gene="ispG"
                     /locus_tag="VF531_14140"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7715151.1"
                     /translation="MKPRRLTRPVRIGSLTVGGNHPIAIQSMTNTDTRDRERTMEQIK
                     ELAATGCEIVRLAVPDAEAATQLGYYVQNATVPLVADIHFDYRLALTAIEAGISKLRI
                     NPGNIGGTDRVRLLAAKARERGIPIRIGVNAGSLEPALLDKYHGPTPEAMVESALGHI
                     RLLEDEGFQDIVVSMKSSTVSDTVIAYQLLAEKVSYPLHIGITEAGTWFKGSVRSAAG
                     LGILLWEGLGDTLRVSLTGDPLREVVVAREILQSLGLRKFGATVVSCPTCGRCRINLE
                     QLAEQVELLASGIQKPLKIAVMGCAVNGPGEAREADLGIAGGKGEALLFRKGEVLRKV
                     PEAEIIPALRAELEKLVD"
     gene            complement(1637..2725)
                     /locus_tag="VF531_14145"
     CDS             complement(1637..2725)
                     /locus_tag="VF531_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX7715152.1"
                     /translation="MGAFNLNSIWLAILLLNIVVAVHELGHFLAARACGVKVHEFSIG
                     FGPLVTKFNWKGVQYSLRWILIGGFCKIAGMDIALEGEPEQESVPQEKLFYFKPLWQK
                     LIILVAGPFFNLLMAILVLFGTAAFIGLPGPTSNALVYQVTAGSPANQAGIRKGDQIV
                     ALNRKSIQGTEIAKIIGKSGGKPLQVTVRRNGVLLTKELTPKYDTAAKRYTMGIVYGV
                     LPKLERVSLGQAAGKALSLPVTMVKLVGMIFNGRAKADLSGPIMVVDMVDQSLQLPLI
                     YILVYLLSFSVTLNMSLFLFNLIPIPLPILDGGWVAILLLERLFHHEFSTEQKAAAQM
                     LGLLLVIILAVMATYGDILRIVRRLLGG"
     gene            complement(2730..3896)
                     /locus_tag="VF531_14150"
     CDS             complement(2730..3896)
                     /locus_tag="VF531_14150"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7715153.1"
                     /translation="MKRLAILGSTGSIGRQTIEVVREFPERFQVTALAAGSNLELLAG
                     QARVLKPQYIALGREDLVPRLRELLGDAKIQILTGLDGLITLATLPEVDQVVTAVVGS
                     LGIRPTLAAINAGKSVALANKETLVAAGSIIMAAAARQKATIIPVDSEHSAVFQCLEG
                     RDRNQVSRILLTASGGPFRSYRPEDLEQVTLKQALNHPNWSMGGKITIDSATMINKGL
                     EIIEAHWLFGMDFHRIAVVLHPESIVHSMIELRDGAILGQMGLCDMRLPIQLALTYPE
                     RLENSFPKLDPTQGMELHFEPVNTKLFPAIPLAYQVGKAGGSLPAAFNAANETAVEAF
                     RQGRLKFTGIVRIVSEVIGHHQKENFSANPDLDEILEVDNWARATAGKLIELGR"
     gene            complement(4292..5359)
                     /locus_tag="VF531_14155"
     CDS             complement(4292..5359)
                     /locus_tag="VF531_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7715154.1"
                     /translation="MTRELKLIFILVSIGIGLIFGLKFLFPFIAPFLLGAFFACLMEP
                     LVRKITILFGVSRRLAVGLVLSAWILFILSLAVIMVLALVQEAQRLIPGIPLFVNKLT
                     VVTEEALRFLEHLPQLAQILKSFSLDPAALERILRALLGWILGFLPQFPGIFFAISLG
                     GITAYLFSRDKERISRIFYQNLPRVWRPATVQIKDEVITTLGRFIRAEFGLVLMTTLL
                     TTIIFFVLGIPGCFVYGMLAGVLDFFPVVGPGILYFPLALIQLLFGDYIRAFALIGGY
                     FLLILVRQIAEVKLVGANLNFYPLLTIVVFYVGLKIFGFEGIIFGPVLIVTLRAIYHG
                     LTEASRPLGARQQATGNREQA"
     gene            complement(5487..6335)
                     /locus_tag="VF531_14160"
     CDS             complement(5487..6335)
                     /locus_tag="VF531_14160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7715155.1"
                     /translation="MSKRIFWGGLAALSSVVIIWMGGIYYAVIVGVLTLLALHEYAEL
                     LKKQNLRPQTALLAIISIGLLTVIFILAQNPRMLPGQFPKNCERFLTLALVIVFFITL
                     GFELFRGDPDQGLINAAANLFGAVYIGFMFAYILLLRFISSENGLFYLIFTTFVTWAN
                     DTAAYFIGVKFGKHKLSPRISPKKTVEGSFGGLAGGVVTAFFLATLFQEPLFFFGILG
                     AIVVIAGQFGDLIESIIKRNAGVKDSGSFLPGHGGVLDRFDSLLLSAPVVYYAIVYVA
                     PFFNLT"
     gene            complement(6328..7107)
                     /locus_tag="VF531_14165"
     CDS             complement(6328..7107)
                     /locus_tag="VF531_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX7715156.1"
                     /translation="MDFTSWLNRIKRSKNHLTPKRVPVHVAIIMDGNGRWAQQRGLPR
                     SVGHRAGMERVRDAVESCLEAGVHYLTLYAFSTENWHRPPAEVNYLMELFEEALRDEI
                     PKLHQKGVRIRFIGLKQGLRPTLIKSMEQSEQLTSANQALTLNIALNYGGRSELVAAA
                     KGLMRATQKGELKPEDLTEAELAKHLFTAGQPDPDLLIKPGREYRISNFLIWQLAYAE
                     LYFSDVYWPDFGKTEMEKAFIEYSRRERRFGGVGEGKHHHE"
     gene            complement(7312..>7429)
                     /locus_tag="VF531_14170"
     CDS             complement(7312..>7429)
                     /locus_tag="VF531_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7715157.1"
                     /translation="ISEDEVKKGLDDAQKLTDKFISEVDKLTEQKEKEIMDV"
BASE COUNT         1977 a         1978 c         1749 g         1725 t
ORIGIN      
        1 tcgaaggagt atagatcctt catcacgaac tccctccccc gcatcaaacc gaaacggggt
       61 cttatctcat cccggtattt attttgaatc tggtataaac gaagcggcag atcacgataa
      121 gaacggactt cgttcttgac cagggtggta atgagttctt catgggttgg tcccagacaa
      181 aactcacggt tatgccggtc ctttaaacgg aacatttcat cgccgtatac ctcccagcgt
      241 ccgctctcct gccagatctc ggcaggttga acgataggca gcgccaattc ctgaccgccc
      301 tcccgatcca tctcctcccg gacgatctgg gagatcttcc ggagcaccct ttgggccaaa
      361 ggcagataag tatagattcc tgtagccgat ttgcggatga atcccgctcg aagcatcagt
      421 ttgtggctga caatctccgc ttctgccggg gtttcccgca gagttggcgc aaataatttg
      481 gattgaaaca taaccgtacc cccaattaat ctacgagttt ttccaattcg gcacggagtg
      541 ccgggatgat ctcggcctcc ggaactttcc ggagaacttc acctttacgg aagagcaacg
      601 cctcgccttt accgccggca atccccagat ccgcctcccg ggcttcaccg ggaccattta
      661 cagcacagcc cattacggcg attttcaagg gtttttgaat tccggatgcc aagagctcca
      721 cctgttccgc caattgttcc aggttaatcc ggcaacggcc gcaggtcgga caggatacta
      781 cggttgctcc aaacttccga agccctaggg attgcaggat ttcacgggcc acaaccacct
      841 cacgaagcgg gtccccggtg agtgaaaccc ttaaggtatc gcccagacct tcccagagca
      901 aaattcctaa tcccgcggcg gaacgcaccg atcccttgaa ccaggtcccc gcctcggtta
      961 tcccgatatg taacggatat gagacttttt cagccaataa ttgatatgca atgaccgtat
     1021 ccgatacagt ggaagatttc atcgaaacga caatatcttg aaaaccctcg tcctcaagca
     1081 aacggatatg gccgagagcg ctttccacca tggcttcagg ggtcggacca tgatacttgt
     1141 ccaaaagagc gggttccagc gacccggcgt tgactccgat ccggatggga atccccctct
     1201 ccctggcctt ggcagcaagc aaacggaccc gatctgtccc tccgatattc ccgggattga
     1261 tccgcagctt ggagatgcct gcttcaatgg cggtcagggc cagacggtaa tcaaaatgga
     1321 tgtcggccac caatgggact gtggcgtttt gtacataata gcccagttgg gtggctgcct
     1381 cggcatcagg tacagccaac cgtacaatct cacatccggt agcggcaagt tccttgattt
     1441 gctccattgt ccgctcgcga tcccgggtat cagtattcgt catcgactgg atcgcaatgg
     1501 gatgattacc gccgacggtt agggatccga tccgtaccgg acgtgttaaa cgacgtggtt
     1561 tcatggttat caactcttgt taaattatat cataagcaag cagggcttga aaggatgatt
     1621 cttcgaaggc ggcttgtcac ccacccaata gacggcggac aatcctcaaa atatcaccat
     1681 aagttgccat aaccgcgaga atgatcacga gcagcaaccc gagcatctga gcggctgctt
     1741 tttgctcggt agagaattca tgatggaaaa ggcgttccag taataaaatt gctacccaac
     1801 cgccatccaa aatcggcaac ggaataggaa tcaggttgaa cagaaagagc gacatattca
     1861 gagtcaccga aaatgagagc agataaacca ggatgtaaat caagggtaat tgtaaagatt
     1921 ggtccaccat gtccacaacc ataatcgggc cgctgagatc ggctttggca cggccattga
     1981 agatcatgcc aaccagcttc accatagtca caggtaaaga caatgcctta ccggcggcct
     2041 gacccaatga gacccgctcc agtttgggga gaactccata gacaataccc atggtatatc
     2101 gtttagccgc cgtatcgtat tttggggtaa gttctttggt gaggagcact ccattacggc
     2161 gaactgtcac ctgcaggggt tttccccccg atttaccgat gattttggcg atctctgtac
     2221 cctggattga ttttcggttt aaagcgacga tctggtctcc cttccggatt cctgcctgat
     2281 tagcggggga acctgcagtc acttgataaa ccaaagcgtt cgaggttggc cccggaaggc
     2341 cgatgaaagc ggcggtcccg aataatacca aaatcgccat caataaattg aaaaaaggac
     2401 ctgccactaa aataatcagc ttttgccaga ggggtttgaa gtaaaaaagt ttctcctgcg
     2461 gaacggattc ctgctccggt tcaccctcaa gagcgatgtc cattccggcg atcttacaaa
     2521 atccacctat cagaatccag cgtaaggaat actggacgcc tttccagttg aactttgtga
     2581 cgagtggccc gaaaccgatg gaaaactcgt gaaccttcac tccacaagcc cgggcggcca
     2641 agaaatggcc caactcatga accgccacga cgatgttgag taatagaatt gccagccata
     2701 ttgaatttaa gttgaaagcg cccaaaagtc tacctcccta gttcgatcaa cttccctgcc
     2761 gtcgctctgg cccaattatc tacttccaat atttcgtcca gatcgggatt ggccgagaag
     2821 ttttcttttt gatggtgccc gatcacttct gaaacaatgc gtacgattcc ggtgaacttc
     2881 aatctacctt ggcggaaagc ttccactgcc gtttcattag cggcattgaa cgctgccgga
     2941 aggctaccgc cggccttacc cacttgatat gccaggggaa tggccggaaa tagcttagtg
     3001 ttgaccggtt caaaatggag ctccatccct tgcgtcggat ccagtttcgg aaaactgttt
     3061 tcaaggcgtt cgggataagt caatgccagc tgaatcggga gcctcatgtc gcataagccc
     3121 atttggccaa gaattgcgcc atcgcgaagc tcgatcatgg aatgcacgat gctttcggga
     3181 tgaagtacca ctgctatccg gtggaaatcc atgccaaaca gccaatgagc ttcgatgatc
     3241 tccaggcctt tattgatcat tgtcgccgaa tcaatggtta ttttgccgcc catgctccaa
     3301 ttgggatggt ttagtgcctg cttgagggtg acttgttcca aatcctccgg ccggtacgaa
     3361 cgaaagggtc cgccggatgc tgtcaacaga atccgggaaa cctggtttcg atcacggcct
     3421 tccaagcatt gaaagacggc gctgtgttcg ctgtctaccg ggatgattgt cgctttttgc
     3481 cgggctgccg cggccataat gatcgaaccc gccgccacca atgtctcttt gttggccagt
     3541 gctaccgact taccggcatt gatcgcagcc agtgtcggcc ggataccgag tgacccgact
     3601 accgccgtaa cgacttgatc cacctccggc agggtggcca aggtaatcaa tccgtccaaa
     3661 ccagtaagga tctgaatttt ggcatctcca agcaactccc tgagccgggg aaccagatct
     3721 tcccttccca atgcaatgta ttgcggttta agaactcgtg cttgacctgc caaaagttcc
     3781 aggttggaac cggccgccaa agctgttacc tgaaaacgct cgggaaactc gcggaccacc
     3841 tcaatggtct gacgtccgat ggaaccggta gaacccaata tcgccaatcg tttcaaaatt
     3901 ctgtcacacc acccgtagcg aagagtcaca agtcaaaagt caaaaaaatc tgtaattcta
     3961 tagtaaaagt ccggcccagg gctgtctaaa agcaaacaat ggataaccgt ccttatcccc
     4021 actaggccct ttaggagcct ttctacgcct ggaaaaccat ctcaagcctc atgaatgggg
     4081 ctgggtaata catccagttt tgcccagcct gttttaacgg gctttagaat ggtttttaac
     4141 ttagcccggg gtttctcgcg tcccatggac gcgcaccccg ggtggacgga atggcaaatc
     4201 agacttttga gacagccccg gacatagggt tatactgaaa gctaatagag aacgcgggtg
     4261 agttgctgcg cgtcccgcag gacgcgatgc ctcacgcctg ttccctgttg cctgttgcct
     4321 gttgcctcgc gcctaacggg cgcgaggcct ctgtcaggcc atgatatatg gcgcgcaagg
     4381 tgacgattag taccggcccg aagatgattc cttcaaagcc gaagatttta agccccacat
     4441 agaatacgac gatggtaagc agcgggtaaa agtttaaatt agctcccacc aacttcacct
     4501 cggcgatctg ccgcaccagg atcaggagaa aatacccgcc tatcaaagca aatgcccgga
     4561 tgtaatcacc aaacaatagt tgaatcaagg cgaggggaaa atagagtatt cccggaccaa
     4621 ctacagggaa aaaatccagc acccccgcaa gcattccata gacaaaacat ccgggtattc
     4681 ccaatacgaa aaagatgatc gtagttagca gggttgtcat taataccagc ccaaattccg
     4741 cccggataaa acggcctaaa gtggtgatca cttcatcttt aatctgtacc gtggctggtc
     4801 gccaaaccct cggcaagttt tggtaaaaaa ttcgacttat tctttcttta tcccggctga
     4861 aaaggtatgc cgtgattcct cccagtgaga tggcaaagaa aatcccggga aactgcggta
     4921 aaaacccaag gatccaaccg agtaaagccc ggaggatccg ttccagtgcc gccgggtcca
     4981 aagaaaagct tttgagaatt tgcgccaatt gcggcagatg ctccagaaag cgtaatgcct
     5041 cttcagtcac gacggtcaat ttattgacga aaagcgggat accgggaata agccgttgag
     5101 cttcttgaac caaagccaga accatgataa ccgcgagact gagaataaaa aggatccagg
     5161 cggataggac caaacctaca gccaatctcc ggctgactcc aaataaaatt gtaatttttc
     5221 gtaccaacgg ttccatcagg caggcaaaaa atgcgcccaa caaaaagggg gcgataaatg
     5281 ggaagagaaa tttcaaccca aagatcagcc ctattcctat cgagacaagt atgaaaatga
     5341 gcttgagttc ccgggtcatt cggcccacct tgattcaaaa ctgccgaatc aactttgaaa
     5401 acttattgcc gtaccgatat ttccttaagg atcactaggg ataaacttat atcaaaacca
     5461 aatcgggacc cggttccgag gatagattaa gtcaagttaa aaaaaggcgc tacgtagaca
     5521 atggcgtaat acacaacagg tgccgacagc aacaggctgt cgaaacgatc caatacccca
     5581 ccgtgaccag ggaggaaaga accggagtct ttcacgccgg catttcgttt gataatcgat
     5641 tcgatcaaat caccgaattg accggcaatt accacgatag ctcctaaaat tccaaagaag
     5701 aataacggtt cttgaaatag tgttgccaaa aaaaaggctg tcactacccc gccggctaaa
     5761 cctccgaagg aaccctccac cgtttttttg ggactgatcc gcggggaaag cttatgtttg
     5821 ccgaacttga caccgatgaa ataagccgcc gtatcgttgg cccatgtcac aaaggtggta
     5881 aaaataagat aaaacaagcc gttttcgctg gagataaacc tcaacagtaa gatatatgcg
     5941 aacataaacc cgatatatac cgctccgaat aaattggcgg cagcgttgat caggccctga
     6001 tcgggatcgc cccggaataa ttcgaaacca agggtgataa agaagacaat caccagtgcc
     6061 aaggttaaaa aacgctcaca gttcttggga aactgccccg gaagcatccg gggattttga
     6121 gccaaaataa aaatcacagt caataaccca atggaaataa tggccaataa cgcagtttgc
     6181 ggccggaggt tttgtttttt gaggagttcg gcatattcat gcagggcaag cagtgtcaaa
     6241 actccgacaa tcacggcgta atagataccg cccatccaga tgataaccac cgatgacaac
     6301 gccgccagtc caccccagaa aatccgttta ctcatggtga tgctttccct cccccacgcc
     6361 accaaaacgc cgttcccggc ggctgtattc tataaaggcc ttctccattt ctgtttttcc
     6421 aaaatcaggc caataaacat cagaaaaata aagctctgca taagccaatt gccagatgag
     6481 gaagttgcta atccggtatt cacgtccggg tttgatcagc agatccgggt cgggctggcc
     6541 ggctgtgaaa agatgtttgg ccaactccgc ctcggttaag tcttcaggtt ttaattcacc
     6601 cttctgagtt gcccgcatta accccttggc cgcggctacc aactctgatc gaccgccgta
     6661 attaagggcg atgtttaagg ttagtgcctg attggcggag gtaagttgtt cactctgctc
     6721 catcgattta attaatgtcg gacgtaatcc ctgcttaagc ccgataaatc gaatccgaac
     6781 ccccttttga tgcaacttgg ggatctcatc gcgtagcgct tcttcaaaca actccatcag
     6841 ataattgact tctgcgggag gacgatgcca attttcggtc gaaaaagcat agagggttag
     6901 ataatggacc ccagcttcca ggcagctctc aaccgcgtcc cggacacgtt ccatcccggc
     6961 acggtgacct actgatcggg gtaaaccccg ttgctgcgcc caacgtccgt ttccgtccat
     7021 gataatagcc acatgtaccg gaacacgctt tggagttaaa tgattctttg accgtttgat
     7081 tcgattcagc caagaagtga agtccatata ttttgccttt cctggtccgg cagatttcaa
     7141 atccgatgcc gttcaatccc ccgaacaatc aggcagggct agcgcggcgg aagcggaaat
     7201 aagagcttgt ccagatgata atagccaagc caaccggagc ggattcacgc tgaaccggtt
     7261 ccgattggcc gacgatgaat tgcctgtcaa tgatttcttt ccgagtttcg atcagacgtc
     7321 catgatctcc ttctctttct gctcggttaa tttatcgact tcactgatga atttatcggt
     7381 tagcttttgg gcgtcatcga gacccttctt cacttcatcc tcggagatt
//
LOCUS       DASVMB010000505         7127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_208307,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000505 DASVMB010000000
VERSION     DASVMB010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208307"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VF531_14175"
     CDS             <1..554
                     /locus_tag="VF531_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HEX7715158.1"
                     /translation="EQALEELQAVSVQPGFVPLKVALLGEIYMVLEPKVNFEMERTLG
                     RMGVEVERTIHFTDWAYETLLLSIIQPNWRRKYQLMARPYLENFVGGHGVETVAHTVE
                     AGINHFHGVIELAPFTCMPEIIAMQALPAVSRDLNIPVLSVIIDEHSAEAGIQTRLEA
                     FIDLLNYRRRPNPCLSREAVLGR"
     gene            722..1714
                     /locus_tag="VF531_14180"
     CDS             722..1714
                     /locus_tag="VF531_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX7715159.1"
                     /translation="MQLYLGVDVGSVSTNLALINNRGELVKKLYLRSQGNPVGSVQYG
                     LAQIARDLKDFEICGVGTTGSGRLLIGKLLGADLVKNEITAHAVACLNLEPGVQTIIE
                     IGGQDSKLIIIRDEIVSDFAMNTVCAAGTGSFLDQQAARLNMEIGELGTRAAEGRHPV
                     RIAGRCAVFAESDMIHKQQMGHPLEDILAGLCSALVRNFLSNLGKGRELNPPVIFQGG
                     VAANLGIRRAFEEALGCKLIIPEHYDVMGAIGVALLARDAVSGSKEGTRFKGFQIADA
                     PINTSSFDCEGCANRCEVIEMRQAGQLQACLGDKCGKWSGRIQAEHFEPSVRVG"
     gene            1731..2279
                     /locus_tag="VF531_14185"
     CDS             1731..2279
                     /locus_tag="VF531_14185"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEX7715160.1"
                     /translation="MQPKQNPPEPVVPNLVRPGLKILFVGINPGLRSAAIGHNFAGHS
                     NRFWRFLFQSGLTLSQFDALHDRDLLELGYGITNIVSRSTATAAELTRVDFEAGGRVL
                     LDLLSEYRPRIAAYLGKDIYRYLSETSHLDWGLQPQRVVMNVVDFILPNPSGLNRIPL
                     PEQLHYYVELKKLSDRLEKASG"
     gene            2497..2751
                     /locus_tag="VF531_14190"
     CDS             2497..2751
                     /locus_tag="VF531_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX7715161.1"
                     /translation="MIFNPVDYCLFPVALSYEWGIGMEAKVDQDLCISCGLCVSSCPD
                     VFSWGDDEKAEAHNAGITNDNESCVQDAAESCPTDAIKVH"
     gene            2913..3806
                     /locus_tag="VF531_14195"
     CDS             2913..3806
                     /locus_tag="VF531_14195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7715162.1"
                     /translation="MRNYRSRNHLLNLNRLFNRESPSTQEEHSGIDGDSGTLSTVTPT
                     AAEFQEAEHDDAEFETTAPFDSESEYSENTVFPSRFFGQTIVVLICLILVMLLTQSNH
                     PSTRWIRSQLHSAVNASSDATFGYISRTATFQKLAKNLENLVRLEEITKRLSEQRESI
                     PDDALPVSVLPVGTIWPVEGRIVRNFGWQELSGNRREFYPGLEFAINPGTEVMAIRNG
                     TVSAIQIQPQSGGVISLDHGNGLISTYRYLNQLQVRIGQAVRTGTVIGRAAGSVLSFE
                     LRQDNRAVDPLSIIGSQYAAN"
     gene            3793..4680
                     /locus_tag="VF531_14200"
     CDS             3793..4680
                     /locus_tag="VF531_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7715163.1"
                     /translation="MPQIKIGFGALSLFALVLFFGGNWPVLLATLLAFIIHELAHLIS
                     GKLLGYRISELKLTPWGGRMKLDPGFGMEPGSEAWIAAAGPAINLLMVAGVYYLNLLG
                     FASPLLIPWGRLNLLIGGINLIPAFPLDGGRIIHALLAAHFGLPRASRYCRMLNWIIA
                     GFILALGAAQIRRDPGGFIPLFVGFSIFCRSFGDSLPEVFVTRQLLERKKKKLANQGF
                     IKVKPVFVSMNTRVRVPLEHCRSSDYLLFVATDSNGKLVFIPEEAAWEGLLADGYEAC
                     FGQRASATAINKVPTIVHR"
     gene            5155..7017
                     /locus_tag="VF531_14205"
     CDS             5155..7017
                     /locus_tag="VF531_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX7715164.1"
                     /translation="MTSGWEKIRNQLDQVSKPGRYIGEEFNTRIKEGSDTDLRVALAF
                     PDLYEIGLSHLGLRILYELINSQPEMVAERVYAPWPDFQELLRHSGAVLYSLESKRDL
                     NRFDVIGFSLQYELSYTNVLTILDLAGIPLSSRERGSSREYPLIIGGGPCTFNPEPVA
                     EFFDFFIIGEAEDVLIPVLEKVHTWKNDLNAGDKDSLLLELAQMPGVYVPSHFEVGYH
                     PNGKIARIENKTGDRPIRAVVRDFDHAFFPQQWIVPYLDIAHDRVAYEIQRGCTRGCR
                     FCQAGMIYRPVRERTPERVMKGLESLIAGTGYEELSLTSLSSADYSRISELLESACGC
                     FTPQKVNVSLPSLRVDSFSVDLAKRFQSNRKASLTFAPEAGTDRLRRVINKGVTEDDL
                     MQATNAAFRAGWQKLKLYFMIGLPTETEEDLAGIANLAGEVLRLGRQIYPRSGGLRVT
                     VSVSTFVPKPHTPFQWRPQISLTEIEQKQNYLKERLRGRFLEFNWHDPQTSRLEGVFS
                     RGDRRLSTVIRRAWEGGAQFDGWSEQFRYQVWMNAMDAAGLNPDFYTGSREYDEILPW
                     SHLESGVDPEFLKLEDTRATAGLPTQDCRQYSCSACGVCSRLGVSKDLAGEIHG"
BASE COUNT         1714 a         1645 c         1895 g         1873 t
ORIGIN      
        1 gagaacaagc tttggaagaa ctccaggcgg tttcggttca gccgggtttt gttcctctca
       61 aggtggcttt attgggcgag atctatatgg tacttgagcc caaggttaat tttgaaatgg
      121 aacgaacctt gggtcgaatg ggggtagagg tggaacgaac catccacttc actgactggg
      181 cctatgaaac tcttttgctc agcattatac agcctaactg gcgccggaag tatcaattga
      241 tggcccggcc ttacctggaa aacttcgtgg gagggcacgg ggttgaaacc gtggctcata
      301 ctgtggaagc gggaataaac cattttcatg gagtgatcga actcgcccca ttcacctgca
      361 tgccggagat catcgctatg caggctttac cggcggtttc ccgtgatctc aatattccgg
      421 tactatcggt gatcatcgat gagcactcgg cagaagcagg gatccaaacc cgtttggaag
      481 cgttcattga tctcctaaac taccggcgca gacctaaccc ttgcctttcc cgtgaagcag
      541 ttttaggtag atgactcagg gaagggtaac cctagccttc gccggtcaaa aagatgaagg
      601 ataggatgaa aaatataggt tgatggataa aatgaaaaat gaagatagat ggatagaggt
      661 tgaaagatag gggttactct cccctaaagg ggagagcaag agagaggtag gaggaaacgt
      721 agtgcaactt tatttgggtg tggatgtcgg ttcggttagt acgaacctgg ctttgattaa
      781 caaccgtggg gaattggtaa aaaagttgta ccttcggtct cagggtaacc cggtaggttc
      841 ggtgcagtat ggtttggctc aaattgcccg tgatctaaag gactttgaga tctgcggtgt
      901 gggtacgacc ggttcagggc ggttgcttat tgggaaactt ttgggggctg atctcgtcaa
      961 aaatgagatt actgctcatg ccgtggcgtg tttaaatctt gaaccgggtg tacaaacgat
     1021 tattgaaatc gggggtcaag attccaagct gatcatcatt cgagatgaaa tcgtcagcga
     1081 tttcgccatg aacaccgtct gcgccgctgg taccggttca ttccttgatc aacaagctgc
     1141 ccggcttaat atggaaatcg gcgaattggg aaccagggcg gccgaagggc ggcatccggt
     1201 gcggattgcc ggtagatgcg cggtctttgc cgaatccgat atgatccata aacaacagat
     1261 gggacatccg cttgaggata ttctggccgg gctatgcagc gctttggtcc gaaatttctt
     1321 gagtaacctg ggaaaaggcc gtgaattaaa cccccccgtc atctttcagg gaggggtcgc
     1381 cgctaatctg ggaatcaggc gggcctttga agaggcattg ggctgcaagc tcatcattcc
     1441 ggaacattat gatgtgatgg gggctattgg tgtagcttta ctggcaaggg acgcggtatc
     1501 tggttcaaaa gagggaactc gatttaaggg tttccagatc gccgatgctc ccattaacac
     1561 gagtagtttt gactgtgaag gctgtgccaa ccgttgcgag gtgattgaaa tgcgccaagc
     1621 aggacaactt caggcctgtc tgggcgataa atgcgggaaa tggagcggcc gaatacaggc
     1681 cgagcatttc gaaccatcgg ttcgggtggg ttaagatcaa accgatttcc atccaaccaa
     1741 aacaaaatcc tcccgaaccg gtcgttccta acttagtccg gccgggacta aaaattcttt
     1801 ttgtcggcat caatcccggc ttgcgtagtg cggcgatagg ccataatttt gccggacatt
     1861 ccaaccgttt ttggcgtttc ctgtttcaat cgggtcttac cctttcccaa ttcgatgcgc
     1921 ttcatgaccg tgacttgttg gaattgggat atggaatcac caatatcgta tcccgttcca
     1981 ccgctaccgc cgcagaatta acccgcgtcg attttgaggc gggcgggcga gtcctccttg
     2041 atttgctctc cgagtatcgg ccccgaatcg ctgcttatct tggcaaggat atctatcgct
     2101 acctttccga aacctcccat ttggactggg gtcttcaacc ccagagagta gtaatgaatg
     2161 tagtggattt cattcttccc aaccccagcg gtttaaaccg aatcccgctt ccggaacaac
     2221 tccattacta tgtggaactt aaaaaactct cggatcgact tgaaaaggca agtggttagt
     2281 ttcttcgtta aattcataca tactccgata attaagcatt atactattag aaaggcaaca
     2341 gtaacagatg tgaggtacga ggtatgagga aactcacccc gcgatactca tacgttttca
     2401 gtttaaccct acgcccctct ctttcgaaaa agagaggggt cagggtgagt tgttaaagag
     2461 acttcagacc tttggttttc gacacgaggt gagggtatga ttttcaatcc ggttgactat
     2521 tgcctgttcc ctgttgcctt aagctatgaa tgggggattg ggatggaagc gaaagtagat
     2581 caggatctat gtattagttg cggtttatgc gtatcaagct gccccgatgt cttctcctgg
     2641 ggtgatgacg aaaaagccga ggcgcacaat gccggaatta ccaacgacaa tgagagttgt
     2701 gttcaggatg ccgctgagag ttgccctacg gatgccatta aagtccatta gggtttggcg
     2761 gagatctcta attgacggcg caccaacgcg atttatccat tgtcaagccc agccgggctt
     2821 gtttttttta atcaaaggac aaattttcag accgattcta gccaagcctt ctctcccata
     2881 tatatagttt aataaagggg aggagagttc cgatgaggaa ttatcggtct agaaaccatc
     2941 tacttaattt aaaccgcctg tttaataggg aatctccatc aactcaggaa gaacattccg
     3001 ggattgatgg agattcggga acattatcaa ccgttacacc gactgcggcc gagtttcaag
     3061 aggcagagca tgatgatgca gagtttgaaa cgactgcgcc ttttgattcc gaatcggaat
     3121 attccgagaa taccgttttt ccatcgcgct ttttcggcca gaccatcgta gtgttgatct
     3181 gccttatcct ggtcatgctc ttgactcaaa gtaatcatcc ttcgacccgt tggattcgta
     3241 gccaattaca cagtgctgtc aatgcttcat ccgatgcaac cttcggttat atctcacgga
     3301 cagcgacttt ccaaaagttg gccaaaaacc tggaaaattt ggttcgattg gaggagatca
     3361 cgaaaagatt atccgaacaa agggaatcca ttcctgacga tgctctaccc gtatctgttc
     3421 tcccggttgg cacaatctgg ccagtagaag gacggattgt ccgtaatttt ggctggcagg
     3481 aattaagtgg aaaccggcgt gaattttatc ccggtctgga attcgccatc aatccgggaa
     3541 cagaggttat ggctatccgt aacggtacag tgagcgccat tcaaattcaa ccccaaagcg
     3601 gaggggtgat cagcctggat cacggtaacg gcctgatctc cacataccgg tatcttaatc
     3661 agctgcaggt gcggattggc caggcggtcc gcaccggaac ggtcatcggc cgggctgcag
     3721 gatccgtcct atcttttgaa ctccgacagg ataatcgggc ggttgatccg ctaagcatca
     3781 tcgggagtca atatgccgca aattaagatt ggattcggag cactgtccct tttcgccctg
     3841 gtcttatttt tcggcggtaa ctggccggta ctcctcgcca cccttttggc tttcatcatt
     3901 catgaacttg cccatctcat cagtggaaaa cttttgggtt accgcatttc cgaactgaaa
     3961 ctaacccctt ggggcggccg tatgaaactg gaccccggtt tcggaatgga accgggatcg
     4021 gaagcatgga tcgccgcggc gggtccggca atcaatttat taatggttgc cggtgtttat
     4081 tatctcaatc ttttaggctt tgcttcaccg ctcttgatcc cctgggggcg gttgaacctg
     4141 ctaattgggg ggatcaattt aatcccggct tttccactgg atgggggcag aataatccat
     4201 gcccttctag cggcccactt cggtctaccc cgcgccagtc gttattgccg gatgctcaat
     4261 tggatcattg ccggctttat cttggctctg ggtgccgccc aaatcagacg ggacccggga
     4321 ggtttcatcc ctctctttgt aggtttctcc atattctgcc ggtcttttgg tgattcgttg
     4381 ccggaggtct ttgtcacccg gcaactcttg gaacgaaaga aaaaaaaact tgccaatcaa
     4441 ggatttatca aagtcaagcc agtgtttgtt tccatgaata cccgggtccg ggttccgcta
     4501 gaacactgcc ggagttcgga ttacctgctc ttcgtggcaa ccgactcaaa cgggaaatta
     4561 gtattcatac ccgaggaagc tgcttgggaa gggctgctcg ccgacggcta tgaggcctgt
     4621 ttcggacaac gcgcttccgc taccgccata aataaggttc cgacgattgt tcaccgataa
     4681 ccgattatat aaaggttggg ttttaggaac gaattttatt gagagactaa gttaatgaaa
     4741 atatgttgtt aaacggtttc ccgtggaagg ccggtgtcat ttgcatactt gcagaatggt
     4801 tcctgatgcg gtagaattat ccttacaatc gaactggaaa ataattattt aactcatggt
     4861 gctatatgaa tttaccttta agctttattt ttaatatggt tgatctacgg aatccttttt
     4921 gcttatgata gataaaatac ttcaatggcc gcaattaaag ccattccatt ttcgtgtctt
     4981 ttgtgtgttt cgttgtgaat aaaatagctt acaaatagaa actttttaat ttaaaccggt
     5041 ttggtttttt ttcaagagtt aatgcggggg atcaaacgat atctccaaag atcggccttt
     5101 ctttgggata tttttttggg aaagttgcgc gatgggcgca cgggaggaat ttttgtgacg
     5161 agcggatggg aaaaaatacg aaatcaattg gaccaagtga gtaaacccgg gcgctatatc
     5221 ggggaggaat tcaatactcg gatcaaggaa gggtcggata cggatctccg agtggctttg
     5281 gcatttccgg atctatatga aatcggcctt tcccacctgg gcctgaggat tctttatgag
     5341 ttgatcaatt cacaacccga gatggtggcg gaacgggttt acgccccgtg gccggatttt
     5401 caggaacttc tgcggcacag cggtgccgtt ctgtattcac ttgagagtaa gcgggatctg
     5461 aaccggttcg atgtgatcgg attctcatta cagtatgaac tgagttacac caatgttttg
     5521 accatcctgg atctggccgg aattccgctc tcgtcacggg aacgcggatc atcccgggaa
     5581 taccccttga tcattggggg cgggccctgc accttcaatc cggagccggt ggcggagttt
     5641 tttgacttct tcatcatcgg tgaagcggaa gatgtattaa tcccggttct agagaaggtt
     5701 catacctgga agaatgactt gaatgccggc gacaaagatt ccttgctgct ggaattggct
     5761 caaatgccgg gtgtttatgt cccgtcccat ttcgaggtcg gttaccatcc caacggcaaa
     5821 attgcccgaa tcgagaataa aaccggggac aggccgatcc gggcggtagt gcgtgatttt
     5881 gaccacgctt tttttcctca gcaatggatc gttccttatc tcgatatagc tcatgaccgg
     5941 gtggcttacg agattcagcg gggctgcacg cggggatgcc gtttttgtca agccgggatg
     6001 atctaccgtc cggtccggga acgaaccccg gaacgggtta tgaagggact cgaaagtctg
     6061 attgccggaa ccggttatga ggagctctcc ctgacctcgt tgagcagtgc cgattatagc
     6121 cggatcagtg agttgcttga atcagcctgc ggctgtttca ccccgcaaaa agttaatgtc
     6181 tccttacctt cactgagggt cgattcattc tcggtggatc tggcgaaacg ctttcagagt
     6241 aaccgcaagg ccagtctgac atttgccccg gaggcgggca ccgaccggct acggcgggtg
     6301 attaacaagg gtgttaccga ggatgatcta atgcaggcaa ccaatgcggc ttttagggca
     6361 ggttggcaaa aactgaagct ttatttcatg attggtttac ccaccgagac cgaagaggat
     6421 ttggcgggga ttgcgaacct ggcgggagag gttttgcgat taggacgtca gatttacccc
     6481 cgctctggag gactacgggt gacggtgagc gtttcgactt tcgttcccaa acctcacacg
     6541 ccgtttcagt ggcgacccca aatcagttta accgagatcg aacaaaagca gaattatctc
     6601 aaggaacggc tccggggccg gtttctcgaa ttcaattggc acgatcccca gacgagccgg
     6661 cttgaaggag tcttttcccg cggtgatcgt cggttgagta cggtaatccg ccgggcctgg
     6721 gagggtggag cccagtttga cggttggagt gaacaattcc gctatcaggt ttggatgaat
     6781 gcaatggatg ccgccggcct aaatccggat ttttataccg gatcgcggga gtacgatgag
     6841 attttaccat ggtcccacct ggaaagcggg gtggatcccg agtttctcaa actggaggat
     6901 accagggcta ccgcgggttt accgactcag gattgccgcc aatattcctg cagcgcctgc
     6961 ggggtttgca gtcggctcgg ggtaagcaag gatctggcag gtgagattca tggctgagtt
     7021 tcgcatggag tttatgaaaa ccggcaattt aaaataccta tcccacctag agctaatccg
     7081 ggctctggaa cgtgccgccc gccgttcccg gctacccatg gaacatt
//
LOCUS       DASVMB010000506         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_651915,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000506 DASVMB010000000
VERSION     DASVMB010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651915"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VF531_14210"
     CDS             complement(<1..908)
                     /locus_tag="VF531_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7715165.1"
                     /translation="MSFEDLHLIAPLLRAVENEGYQEPTPIQKQAIPVLLEKRDLIGC
                     AQTGTGKTAAFALPILQLLYDQRVAPGRRATRALILTPTRELALQIGESFTAYGKNTG
                     LRHTVIFGGVSQNQQTMALKAGVDILIATPGRLLDLMNQKFINLQQIQIFVLDEADRM
                     LDMGFIHDVKRVLTHLPAKRQTMLFSATMPPEIVTLAQSMLIDPVRISVTPESPTVDA
                     IEQAVYLVDKQDKQALLIHLLEGQTIRSALVFTRTKHGADKLARKLIHAGIRAEAIHG
                     NKSQNARQQALHDFKTRRTRVLVATD"
     gene            complement(1585..2565)
                     /locus_tag="VF531_14215"
     CDS             complement(1585..2565)
                     /locus_tag="VF531_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715166.1"
                     /translation="MSFKSLIVTALMMILIAIPAWGDVRVEWDSGVNLSSSATASVWD
                     WQTGFRTSGDWTLAPNLSLFLRDRVVRDSRNEDWYGDLERGYLQYESGPVRVKLGRQA
                     VNWGIGWFFRPNDLITPAANALSHEDTRPGMDLATLYWATSPLTATELVAGDRLLAAR
                     TEWRIGPTNLRFLGVDNAEGLKYLGIDFQGGLAGIYGEGRYGWTESQGFENGKPALLL
                     GWSKALGGGKLLYLEAFKDISKLYYGSENYAATGVEIPVDEFTSFTVAGIGNLDDGGC
                     VITGLVTSQINDNLDLNGAIVVLAGSENTEFLTRAGGYRFTLNLGAKYYF"
     gene            complement(2617..3387)
                     /locus_tag="VF531_14220"
     CDS             complement(2617..3387)
                     /locus_tag="VF531_14220"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7715167.1"
                     /translation="MQARKIKWIGLGISMILGMLSVAPWIGAATLSPGEILDRVDRNS
                     QYQSAYTEAAMEITLGGRKITKTLRTWAEGNQKALVEFTSKRDQGTRILKLGDDLWLY
                     SPTAESEVKLSGEMLKQGMAGSDFSYQDALENEKLRELYQANAAGDETIGGRPCYVLE
                     LVAKPGVEVSYYRRKIWVDEERFVGLKEELYAPSGKLLKVSTTEKVELFGSRYYPTVV
                     VMEDKLRKNSSTRFTIDTIQFNVTIPAGTFTRQRLTGR"
     gene            complement(3446..4705)
                     /locus_tag="VF531_14225"
     CDS             complement(3446..4705)
                     /locus_tag="VF531_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7715168.1"
                     /translation="MLQWIRMAWRNLGRHRGRTILSLAVVTIGVLVVILFKGMIDGML
                     DTMYSYNINLNSGHVRVIRPEYTVKERLLSLSYPVGETGKSYSQLIKDIRRLPGVKNA
                     MGRIRFGMMLVNGENQETALGIGAEIDREEQVSHLSRYLKGNGAGRLPRAGSQEILLG
                     MDLMRKLHLGVGSKVNAVFSTSFGSFNVATFRVVGKLASGLQLLDNGAAYIPLDQAMR
                     LLEMDNVVTEIVAYGSTSAQTSRLTREIGTYLRNGNEKLQVIPWDQYNDLIMTMQKAK
                     AAYNFVYTLILLLASFVIFNTLMMVVAERTREIGMLTALGMTPVEIRRLFLCEGLLIA
                     LIGSFTGTVLGGAANWLMSRTGIDLSSAMGMIPKELTFMPRLYPSYSPAVLVFSFLLG
                     VAVTVLAAYLPARRAALLEPTQALRTI"
     gene            complement(4733..5968)
                     /locus_tag="VF531_14230"
     CDS             complement(4733..5968)
                     /locus_tag="VF531_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715169.1"
                     /translation="MFRFYLGLTFKNLFRHKRRTILTALAIAIGIFFYILFDSIMTGI
                     DRDAVNSILDLETGHFQIVAPAPEENQGKINLKYLLPEGGKLAQRVAGLPGVKGTTPR
                     LIFPASLISSIDELPVTGVGVDPKLDPTVFKIRDSVKGRWLRPGESETVVGKKIATLL
                     EIKVGDTITVRTQTKQNTFQALDLTVVGIVDSVDPVVNEGQFFIPLDTAERALSTGTA
                     VSQIAVKTVDYSKMNPAIQAVEKLQVPGENFRIKTWHEVAEAFLSGIQMKQKFIVVLL
                     LMVALIAIIGVVNSTLLASLERVREIGILKAMGMTEGEIVRLFNYEALGLGFLGGLGG
                     VILGVITNLYLVNVGIDLTKMYGEMNMGLSVGKMYGVWNWSGILGAFALGIVISWLAS
                     HFPARKAARVDPAVSLRRI"
     gene            complement(5974..>6210)
                     /locus_tag="VF531_14235"
     CDS             complement(5974..>6210)
                     /locus_tag="VF531_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7715170.1"
                     /translation="QRVAIARALVKQPKLVLADEPTANLDSENSEAILNLMSELNKRQ
                     QVTFVFSTHDPLVMKYARRLITMRDGRITSDERR"
BASE COUNT         1502 a         1787 c         1476 g         1445 t
ORIGIN      
        1 atatccgtcg ccaccagcac ccgggtccgc cgggtcttga aatcatgaag cgcctgttgc
       61 ctggcattct gcgacttatt gccgtgaatc gcctcggccc gaatcccggc atgaatcagt
      121 tttctggcca gtttgtccgc accatgcttg gtccgggtaa acaccagcgc cgaacggatg
      181 gtttggccct caagcaaatg gatgagtaac gcctgcttat cctgtttatc gaccaggtat
      241 accgcttgtt cgatggcatc cactgtcggc gattccgggg ttaccgaaat ccggaccgga
      301 tcgatcagca ttgactgagc aagggtcaca atctccggcg gcatggtggc ggaaaagagc
      361 atcgtctgcc gtttggccgg caagtgcgtg aggactctct tgacatcatg aatgaatccc
      421 atgtccaaca tgcgatcggc ttcgtccaac acaaatatct gtatctgttg caggttgatg
      481 aacttctgat tcatcaaatc cagcaatcgc ccgggagtag cgatcagaat atcgactcct
      541 gcttttaagg ccatggtctg ttggttctgt gagacacctc caaagatcac cgtatgccgt
      601 aggccggtat tcttgccgta ggctgtgaaa ctctcaccga tctgtaacgc caattcccgg
      661 gtgggagtca ggatcaaagc ccgagtcgcc cgcctaccgg gggcaacacg ctgatcgtac
      721 aacagctgga gaataggcag ggcgaaggcc gccgtctttc cggtacctgt ctgggcacag
      781 ccgatcaggt ccctcttttc caacagtacg ggtatggctt gcttctgaat gggcgttggt
      841 tcttgatagc cctcgttttc caccgccctc aaaagggggg cgattaaatg caaatcttca
      901 aatgacatct atttctcctt tgttcaacaa cccgataagg aaccgtctaa aagtagtacg
      961 gtattaaatc aagtgatatt cgggacggta tcctggaatg atagcctgac cctgcttgat
     1021 tatacaccat ttccccgagt tttgctttct ttttgacaat ttgtaataaa accggacgaa
     1081 aaaacacctg taaaggatag ctattttatc aagcagaacc aatggatagt agcataaaaa
     1141 atcccggaac acgtcggaac cgggatgagt acaatttttt aagtcataaa gctatatttc
     1201 tagtaatgtt gatctgacat atgcaataag cttattctcg ggccaaccac ccctatcccc
     1261 tcctcgagga ggggaattag atatcgaatg cggggacact ccgccccccg gcagcattta
     1321 catgctgcac ttgctttttg atagtctgga ccaaccattt cagaaaacat agttaagaaa
     1381 aaatcccgga acacgtcgga accgggatga gtacactgca cttgcattcg atacttagga
     1441 tcaaccattt ctgaaacaat agctgaattt tgtttattaa tcctttattt ttttaatccg
     1501 ataaatccga caaatccgtt aaatcctgat ccaatcctga ttaatcctgg ttaatcctgg
     1561 tccaatcctg gttaatcctg gtcgtcaaaa gtaatatttc gccccaagat taagggtaaa
     1621 ccggtaaccc cccgcccggg tcagaaactc cgtattctct gaacccgcga ggactacgat
     1681 tgcaccgttc agatccagat tgtcattgat ttgcgacgtc accagtcctg taatgacgca
     1741 gccgccatca tccaggttac cgatgccggc aactgtaaac gaggtaaact cgtctaccgg
     1801 gatctccacc cccgttgcgg cataattttc gctgccgtaa tacagcttac tgatatcctt
     1861 gaaggcctca agatataaga gcttaccgcc acccagggct ttactccatc ccaggagcaa
     1921 agcgggcttc ccgttctcaa acccttggga ttccgtccaa ccgtaacggc cctcaccgta
     1981 gattcccgcc agaccaccct gaaaatcaat gcccagatat ttgaggcctt ccgcattgtc
     2041 aacccccaga aaccggaggt tggtggggcc gattctccat tcggttctgg cggcgagcag
     2101 cctgtctccg gccaccagct cagtggcagt gagcggagag gtagcccagt aaagagttgc
     2161 caggtccatt ccgggacggg tgtcctcgtg gcttaaggca ttggcagccg gtgtaatcag
     2221 gtcattcggc ctaaagaacc agccgatccc ccagtttaca gcctgtcgtc ccaatttcac
     2281 ccggaccggt cccgactcgt actgcagata accccgttca agatcgccat accaatcctc
     2341 gttccgggag tcacggacca ctcgatccct tagaaacaag gagagatttg gggcgagggt
     2401 ccaatcaccg ctcgttcgga atccagtctg ccaatcccag acagaggcgg tggctgaact
     2461 gcttaggttc acgccactat cccattccac ccggacatcg ccccaagccg gaatggcaat
     2521 caagatcatc atcaatgccg ttactatcag cgatttaaaa gacatagcct taacctcctc
     2581 accataccat accttacggc tacaatctac ccggaattat ctcccggtga gccgttgccg
     2641 ggtgaaagtc ccggccggga tggtcacgtt gaattggatg gtgtcgatgg tgaaacgggt
     2701 actggaattc ttgcgcaact tatcctccat caccacaacg gtgggataat agcggctgcc
     2761 gaaaagctcc accttttcgg tggtcgagac cttgagcagt ttaccgctgg gagcgtagag
     2821 ctcctccttc aaacccacga aacgttcctc gtccacccag atcttgcgcc ggtaataact
     2881 gacttcaact cccggtttgg ccaccagttc caacacatag cagggacgtc cgccgatggt
     2941 ctcgtctccg gctgcattcg cctgatacaa ctcacgcagc ttctcattct ccagggcatc
     3001 ctggtaggaa aaatcgctcc cggccatccc ctgcttcaac atctcacccg agagcttgac
     3061 ttcactctcc gcagtaggag aatagagcca gaggtcatcg cccaatttca ggatgcgggt
     3121 tccttgatcc cgtttactgg tgaactccac caaggctttt tgatttccct cagcccacgt
     3181 acgcaaggtt ttcgtaatct tcctgccgcc aagggtgatc tccatcgccg cctcggtata
     3241 ggccgattgg tactgactgt ttcgatccac ccgatcgaga atctctccgg gggagagcgt
     3301 cgcagcgccg atccaagggg ctaccgaaag cattcctaaa atcatactaa tccctaatcc
     3361 gatccattta atctttcgtg cctgcatgat aggacctccc tacaatcaat gatttttcac
     3421 gtatcaaaaa gggcaagtca gaactttaaa tggtccgtaa cgcctgggtc ggttccaata
     3481 acgcggcgcg gcgggccggc agatacgcgg caagaacagt gaccgccact cccagcaaga
     3541 agctgaatac cagcaccgcc gggctgtatg atggatagag ccgcggcatg aaggtgagtt
     3601 ccttcggtat cattcccatc gccgatgaga ggtcgattcc ggtgcgggac atcaaccagt
     3661 tggccgcgcc tcccagaacc gtcccggtaa agctgccgat cagggcgatt aacaaacctt
     3721 cacacagaaa gagccggcga atttcaacgg gagtcattcc gagagcggtt agcatcccga
     3781 tctcccgggt gcgttccgcc accaccatca tcagggtatt gaagattacg aaggaggcta
     3841 ataacagtat caaggtatag acgaaattat aggccgcctt cgccttctgc atggtcatga
     3901 tcagatcatt atactggtcc caggggatca cctgcagttt ttcgtttcca ttccgcagat
     3961 aagtcccgat ctcccgcgtc aaccggctgg tttgggcact ggtgcttcca taggcgacaa
     4021 tctcggtgac cacattatcc atttctaaca gtctcatggc ctgatcaagc ggaatatatg
     4081 ccgccccatt atccaatagt tgtaaaccgc tggcgagttt acccacgacc ctgaaggtgg
     4141 cgacattgaa tgaaccgaac gacgtgctga acacggcatt caccttcgaa ccgaccccca
     4201 ggtgcagctt acgcatgaga tccataccca acagaatctc ctggctaccg gcccggggca
     4261 gcctaccggc tccgttaccc ttcagataac ggctcaggtg actcacctgt tcctcccggt
     4321 caatctcggc accgatgccc aaagccgttt cttgattttc cccgttcacc agcatcatcc
     4381 cgaagcgaat ccggcccatg gcgtttttca ctcccggcaa acggcggata tccttgatca
     4441 attgcgaata ggacttgccc gtctccccaa ccggatatga aagtgaaagc aaacgttctt
     4501 tcactgtata ctccggccgg atcacccgga catgaccgga gtttagattg atattataac
     4561 tgtacatggt atcgagcatc ccgtcgatca tccctttgaa gaggataaca accaggacgc
     4621 cgatggtcac cacggctagg ctcaagatcg tccggccgcg gtgccggccc aggttacgcc
     4681 aggccatccg gatccattgc agcatcgtaa aaaccccctt tatagacctt cgctaaatcc
     4741 ggcgcaaact gacggcagga tcaacccggg ccgccttgcg ggcaggaaaa tggctggcca
     4801 gccaggatat aacgatacca agggcaaaag ctcccaatat ccccgaccaa ttccaaacgc
     4861 cgtacatttt tcccaccgac aaacccatat tcatctcgcc gtacatcttg gtgagatcaa
     4921 ttcccacatt caccagatac aggttggtaa tgacccccag gattaccccg ccaagaccgc
     4981 ccaggaaacc gagacccaaa gcctcgtaat taaagagccg cacaatctcc ccttcggtca
     5041 tgcccattgc cttcaggatc ccaatctccc ggacccgttc cagggatgca aggagggtcg
     5101 agttgactac gccgatgatg gcgatcaacg ccaccatcaa gagcagcacg acaatgaatt
     5161 tctgtttcat ctggattccg gagagaaagg cttcggctac ctcatgccag gtctttatcc
     5221 ggaagttctc ccccggtacc tgtagctttt ccacagcctg aatcgcaggg ttcatcttgc
     5281 tataatcaac ggtttttacg gctatttgag aaacggcggt accggtcgag agcgcccgtt
     5341 cggctgtatc caggggaatg aagaactggc cttcattgac caccggatca acggaatcca
     5401 ctatccctac aaccgtgaga tccagggctt gaaaggtatt ttgcttagtc tgggtacgga
     5461 cggtgattgt atccccaacc ttaatctcaa gtagcgtggc aatctttttc cccactacgg
     5521 tttcggactc accggggcga agccaacggc ctttcacgga atcacgaatc ttaaaaaccg
     5581 tcggatccag cttcggatcg actccaaccc cggtcaccgg cagctcatcg atgctgctga
     5641 tgagggaggc cggaaagatc agccggggag ttgtcccctt gacaccgggc aggccggcca
     5701 ctcgctgagc gagcttccca ccttcgggga gcaggtactt aagattgatc ttgccttgat
     5761 tctcttccgg ggccggggcc actatttgaa aatgtcccgt ctccaggtcg agtatgctat
     5821 tgaccgcatc ccggtcgatt ccggtcataa tcgagtcgaa gagaatataa aagaagattc
     5881 caatggcaat ggccagagcc gtcagaatgg tccgccgttt atggcggaag agattcttga
     5941 aggttaagcc taaatagaac cggaacattc cgactacctc ctctcgtcgc ttgtaatcct
     6001 accgtcacgc atggtgatga gcctgcgtgc atacttcatc accaagggat catgggtgga
     6061 gaaaacaaag gtaacttgtt gccgtttgtt taattcgctc atcaaattca ggatcgcctc
     6121 ggaattttcg gaatccaaat tggcggtggg ctcatctgcc agcaccaatt tgggctgttt
     6181 caccagcgcc cgggcgatgg ccacccgttg
//
LOCUS       DASVMB010000507         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_651970,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000507 DASVMB010000000
VERSION     DASVMB010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651970"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF531_14240"
     CDS             complement(<1..359)
                     /locus_tag="VF531_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7715171.1"
                     /translation="MKKVLSVVLALAMVLAVASFAMAATVSYTGSVKAEYNSINGLNN
                     GYNDTYFTVDYTKDFQNGWSAGAQVKVFAGQTAEQLDAVTNDDLDSLLESDAVNFTGK
                     GFVKYTADMWDIALQTG"
     gene            complement(924..1283)
                     /locus_tag="VF531_14245"
     CDS             complement(924..1283)
                     /locus_tag="VF531_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715172.1"
                     /translation="MKALIVDDAALARTLLRKLLEGNGFQEIVEASNGDEAVQLYQSS
                     KPDLVTMDITMPGMDGVTAIKKIIEIDPLANIIVCSALGEKNIVLEAIQSGAKHFIVK
                     PFDNEKVISIVKAVLEN"
     gene            complement(1502..2332)
                     /locus_tag="VF531_14250"
     CDS             complement(1502..2332)
                     /locus_tag="VF531_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX7715173.1"
                     /translation="MTAAGEPVCVDEQVILEICRILNQHLGFTYGGSKRYLISSRLNH
                     RLRELNLPGYSDYLQVLWQDPREIDRLFDLLTTHVTGFFREPDQYQILSRELAPMLMK
                     RFAITKKLRCWSAGCSSGEEAYTLAMVLSESLGPGWDIKILASDISREILTEGMSGIY
                     AAPGMAEIPQSLARKYFTKSPDGATFQVRPELREQVVFRRINLNETFEIPGHIHFALI
                     FCRNVFIYLSVAARERIIEGFHSVLEPGGFLFVGHSEPLNILDNKRWRPFKNCIYQKV
                     "
     gene            complement(2365..2874)
                     /locus_tag="VF531_14255"
     CDS             complement(2365..2874)
                     /locus_tag="VF531_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7715174.1"
                     /translation="MTMEDRTTDILNNQFMVAANAVQQMIFCVDTEEFGVDVAKVQEI
                     IRYQKPFKVPNTPEFVSGVISFRGEVIPVLDLRRIFGLGSVQYDGFTVILVVQTGAKT
                     FGMIVDRVLDIMNILEANIQNPPELGAKDKTRYLRAMGQMGQRLILMLDLEKIVNFRE
                     FEDSLNQEI"
     gene            complement(2939..>3949)
                     /locus_tag="VF531_14260"
     CDS             complement(2939..>3949)
                     /locus_tag="VF531_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7715175.1"
                     /translation="MRFPKIVRDVAKRSGKFADLHLLGEETEVDKEIAEKLVDPLTHL
                     IRNAVDHGLETGSERKAIGKPETGIVTLGASQEGDYIVFTVADDGKGLDIPKIKAKAA
                     QAGISPPGEALSDDEAMKLIFRPGFSTADQVSEISGRGVGLDVVESSLKGLKGDVEVE
                     SHPSRGTTFRLKVPLTLAIIQSFLFQIGDQTFAVPSAEVVENLAIDHRGLHRIGNKYF
                     LNLRQETIPVIDLRMLFQTDRPVEFTETARPSIVKELTGTKAFAVSGSWDKTPLVIVK
                     SGRYKLGLMVDQLIGQEELMIKQINRALDENPLISGASLLGSGGLTLILNIREIIHSA
                     ML"
BASE COUNT         1047 a         1047 c          826 g         1029 t
ORIGIN      
        1 aaaccggtct gtaaagcgat atcccacata tcggcggtgt acttcacgaa gcctttgccg
       61 gtaaagttga cggcatcgga ctccaaaaga gaatccaaat catcatttgt tacggcatcc
      121 agttgttcag cggtttgacc ggcaaaaacc ttaacctggg cgccggcaga ccaaccattt
      181 tggaaatcct tggtgtaatc aacggtgaaa taggtgtcgt tgtaaccgtt attcaggccg
      241 ttaatgctgt tgtattcagc cttcacagaa ccggtgtagc taacggtagc agccattgcg
      301 aaagaagcaa cagctaaaac catcgctaaa gctaaaacta cggataaaac tttcttcaag
      361 agaataccct cctttaaact ttgcaattaa tatttaaaac atgaaacgcg attctaggtg
      421 ccggtcaacc acctccttat gcttcccggc aactttagct tttcaggtta cgctaaagaa
      481 accactgaaa gcgaatctaa taaatctatt ctctaagacc tttttaattc cttcttgggc
      541 gataaaaatt tttcgcccca gccgggagat ctcccccaaa cctgatcttg atccactaaa
      601 agagtcttta aaatacggaa aaaatcccga attgcctggc aggatttgtc tcaaacattc
      661 cctaaccatt cgctttaagt taacaaaata aaaaatactt caatgaccgc aattaaagct
      721 aatcccattt tcgtgtcttt ggtgtgtttc gttgtgaata aaataggctt acagatagaa
      781 acttttaacc acgaaaatca ccaaatacac gaaatgtctc ttcgggcgat atttttggac
      841 ccgaaaaaag atggttctct atataaagat ttgcttcatt ccttcacaat tattcgtaaa
      901 agctggggat aatagcatta attttaattt tctaaaaccg ctttgacgat actgataact
      961 ttttcattat caaacggctt gacgatgaag tgctttgcac cggactgaat cgcctcaaga
     1021 acgatgtttt tttcccccag ggcgctgcag acaatgatat tggccagcgg atcgatctcg
     1081 attatttttt tgatggccgt cactccatcc ataccgggca tggtaatatc cattgtcacc
     1141 agatccggtt tggagctctg atagagctgg acggcttcgt cgccgttaga agcctcaacg
     1201 atctcctgaa aaccattccc ctctaacagt tttcgcaata aagtgcgggc caaagctgcg
     1261 tcatccacga tcaaagcctt caaaaccgtt ccccccgctc aacctgtttt ctacatctca
     1321 gaatcaactc aaattccaat caagccattt tgattccata tttcaatctt taccctttgc
     1381 cctccgaact ctaaaccttg aactctgaac cttgaactct gaaccttgaa ctccaaactt
     1441 tgaactctaa actctaaacc ccaaactcgg aacccggaac ttccgatttg acaaatatcg
     1501 cttaaacttt ttggtatata cagttcttga aagggcgcca tctcttattg tccaggatgt
     1561 taaggggttc agaatgcccc acgaataaaa atcctcccgg ttccagcacc gaatggaaac
     1621 cttcgatgat ccgttccctg gcggcaacgg aaagatagat aaagacattt ctgcaaaaga
     1681 ttagtgcaaa atggatgtgc ccgggaattt caaaggtttc attcagattt atcctccgaa
     1741 aaaccacctg ttcgcggagt tcgggccgta cttgaaatgt ggcgccatcc ggtgacttag
     1801 tgaaatattt ccgcgccagt gattggggaa tctccgccat cccgggggcg gcgtatattc
     1861 cggacatccc ctcggtcaaa atctcccggc tgatatccga ggccaaaatc ttgatgtccc
     1921 atcccggtcc caaactctca cttagcacca ttgccaaagt ataggcttct tcccctgagg
     1981 aacacccggc agaccaacaa cggagtttct tggtgattgc aaaacgtttc attaacattg
     2041 gagccaattc ccggctcaag atttgatact ggtccggttc ccgaaaaaaa ccggttacat
     2101 gggtggttaa aaggtcaaat aaacggtcaa tctcccgggg gtcctgccac aagacctgta
     2161 aataatcaga gtaccccggc agattgagct cccgtaaacg gtggtttaat cgcgaagaaa
     2221 ttagatatct cttggatccg ccgtaagtaa accccaaatg ttgattgagt atgcggcaaa
     2281 tctccaaaat cacctgttca tcaacgcaga ccggttctcc cgcggcggtc atttttcaga
     2341 ctccagcagg ttcggagttg ccaactaaat ctcctgattc agtgaatcct cgaactcccg
     2401 gaaattgact attttttcca agtccagcat gagaattaaa cgctgcccca tctgacccat
     2461 agccctcaag taacgggtct tatctttggc gcccagttcc ggcgggttct gaatatttgc
     2521 ttcgagaata ttcatgatgt ccaaaacccg gtcgacgatc attccgaaag tcttggcccc
     2581 ggtctggacc accagaatca cggtaaaccc gtcgtactgg acggagccta gtccgaagat
     2641 cctccgcaga tccagcaccg ggatgacttc accgcgaaaa ctaatgactc cgctgacaaa
     2701 ttcaggagta ttgggcactt tgaagggctt ttgataacga atgatctcct ggactttggc
     2761 gacatccacc ccgaactcct cggtatcgac gcaaaagatc atttgttgaa ccgcattggc
     2821 cgcgaccata aattggttgt tcaatatatc ggtggttcgg tcttccatcg tcatcgtttc
     2881 ctcccatgtg ttcacaacac cggcccgtcg ggaaccgttg ttgccggctt tccttcgatt
     2941 acaacattgc gctatgaata atctcccgga tgttaaggat caaagtaagt ccgccgctcc
     3001 ccaagagtga cgccccggat atcaacggat tttcgtccaa tgcccggttg atctgtttaa
     3061 tcatcaactc ttcctggccg atcaattggt ccaccattaa tcccaattta taacggccgg
     3121 atttcacgat caccagtgga gtcttgtccc agctgcccga aaccgcgaaa gccttcgtcc
     3181 cggttaattc tttcactatg gacggccgcg ccgtttcggt gaattcgaca ggccgatccg
     3241 tctgaaaaag catccgcaga tcgatcacag ggatcgtctc ctggcgtaaa ttaaggaagt
     3301 atttgttgcc aatccggtgc aagccacggt gatcaatcgc cagattctca acgacttcgg
     3361 cggatgggac ggcaaaagtc tgatcaccga tttggaaaag gaaggattga atgattgcca
     3421 aggttaacgg aacttttagc cggaaagtgg tcccccggga cggatgggac tcgacttcca
     3481 catccccctt caaccccttg aggctgcttt caaccacatc caggccgacc ccgcggccgg
     3541 agatctcgct gacctgatcg gcagtggaga aaccgggacg aaaaatgagt ttcattgcct
     3601 catcatcact caaggcctct cccggcggcg aaattcctgc ttgagctgcc ttcgctttta
     3661 tcttggggat atccaagccc ttgccgtcat cggcaactgt gaaaacgata taatcccctt
     3721 cctgggaggc ccccaaagta acgattccgg tttccggttt accaatcgct ttccgctccg
     3781 atccggtttc caatccgtga tccacggcat tacgaatcag atgggtcaag ggatcgacca
     3841 gtttctcggc gatctcctta tcaacttcgg tctcttctcc cagaagatgt aagtccgcaa
     3901 atttgccgct gcgtttggcc acatcccgga caattttggg gaagcgcat
//
LOCUS       DASVMB010000508         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_138125,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000508 DASVMB010000000
VERSION     DASVMB010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138125"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..509)
                     /locus_tag="VF531_14265"
     CDS             complement(156..509)
                     /locus_tag="VF531_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715176.1"
                     /translation="MGNELLGFPQIAPPVYPLNEDWEDVGISNGFEDGTEQSRARFTR
                     SRGTWVLHWTALKDSDYQTLMNFWRKDVKGKSQLFNWTHPITGIIYTVRFVEKKPFEL
                     VAPGIWSGEITIREG"
     gene            complement(516..2402)
                     /locus_tag="VF531_14270"
     CDS             complement(516..2402)
                     /locus_tag="VF531_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715177.1"
                     /translation="MSEGSSIQTDMNGLMNQLTQSFDLLASAIKGQIRIIPDNIAKEG
                     NDLTAKLSAVYSEIAREISTNGWEQVAQTLENANTLPDEIKQYTEKLNNRYNELKQAY
                     EEGGLVNLTEKLLPEDTVKRLQESVQSINGYFEDLKKSFTDTSLENILQQLLPEETYQ
                     KLSKLKEQFTEIKTKYDEVSQAFRDGDWEKALKELAPDTYQQFESAKTNFENLKDQIA
                     KIQQAGQEGNWDSVLQQLLPEDMYQRFDQLRQEFLKAQNEYNKLTAAFQEGGLEKALQ
                     QVLPEETYQKLKEQFTEIKTQYDEVSQAFRDGDWEKVLKELAPDTFNQFETVKTNFEK
                     IKDQIAKIQQAGQEGNWDSVLQQLLPEDMYQRFGQLQQEFSKAQDEYNKFAAVFQEGG
                     LGKVLEQYLPSDLFKQIFDFKNIQGDIQNVIGQVVDAFQSGGLDGALKVVFSQDGQSL
                     FQGLQQNFDTWLGGLPDWMGQAANILQSGLNGFFQSFSGTFSGITSFIGNIFQGIGQT
                     TSGWGNLLLNGILALFGIPGLADGGTVDSNSMYVVGEQGPELFVPGVGGTIIPNHSLN
                     GKAESTAVAVPPVTVNVINNSGQPVSTRQESNFDGQRYVVDVWLNALARNVGGLRDVV
                     TAGR"
     gene            complement(2727..2981)
                     /locus_tag="VF531_14275"
     CDS             complement(2727..2981)
                     /locus_tag="VF531_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715178.1"
                     /translation="MTELTKPRAMTRDELKALRTAGLDPACNENGVSIKVNTEMVDWI
                     LDHVYHGFNFGNVPYPECLELATKTYQLTYTLSAVEVKNS"
     gene            complement(3468..4412)
                     /locus_tag="VF531_14280"
     CDS             complement(3468..4412)
                     /locus_tag="VF531_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005382405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HEX7715179.1"
                     /translation="MTQAKGYKAQLVADFETVFGQTPGTVNAVKLPIVSSQLKSKQNF
                     IDSSVITGRRDPAEPAKGNIDLTGTVVVPVDEVAIGYWLKAMFGAPATSGSADPYMHV
                     FKTGDTQPSLVLEQGFTDIGVYELFNGCKVNKFSLTLGGDAELTASIDLMGAMETIRS
                     ASFAGSPANISLAKYNNFQASIKESGANIATVSNVSLNMEFGLAGDSYAIGGNGCRTG
                     IPEGLLKITGNLKAFFEDQSLLNKAMQGTESSLSLKLTNMQHSLELLLPEIVFERNSP
                     GIEGAKGILIDLPFRAYCNNNPENTAIMVTLVNSQAAY"
     gene            complement(4531..5007)
                     /locus_tag="VF531_14285"
     CDS             complement(4531..5007)
                     /locus_tag="VF531_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715180.1"
                     /translation="MFSSLAIHEIITKWRDELLNNPAINDFCQTKYGTHPKIFIGVDP
                     NALPAQSDCPYILIHSGSKTEGMNRKEYRYLIPVEWSISNTAKTVSGQVTELDGLYDM
                     DAFGQLILTALMTASPSNPISYLNYSTLREETFPQFTGRMEVKVSIFPAMGESISY"
BASE COUNT         1396 a         1408 c         1150 g         1562 t
ORIGIN      
        1 ggctcagtca ggccggcaat gttcaattct aaaagaatta gccaaacgcc gtccgatgac
       61 aaacggtttt tttcaataat tgcagctgga tttagtttca gcataccaac cctccctttc
      121 aaatgaggct cactagaaag attcgtggcc tgcatttatc cttcgcgtat agtgatctca
      181 cccgaccaga tccccggcgc caccagttcg aagggctttt tttcgacaaa ccggaccgta
      241 tagattatac cggttattgg atgagtccag ttaaacagct ggcttttccc tttcacatct
      301 ttgcgccaga aattcatcag cgtttgataa tcgctatcct tcaaagccgt ccaatgcaac
      361 acccaagtcc cacgggaacg ggtaaacctg gcccggcttt gttccgtgcc gtcttcaaaa
      421 ccgttgctga tacctacatc ctcccagtct tcattgagtg gatagaccgg aggcgctatc
      481 tgaggaaaac ctagcaattc gtttcccatc ttctcttacc tccctgccgt aacaacatcg
      541 cgcaatccgc cgacattccg ggcgagcgcg ttcagccaga catcaaccac gtaccgctgc
      601 ccgtcgaaat tggattcctg tctggtactc accggttgtc cggagttgtt gatcacattc
      661 accgtcaccg gaggaactgc aacagccgta gattccgcct taccgtttag ggaatgattc
      721 ggaataatcg tcccgccgac tccggggaca aatagctcag gaccctgctc cccgaccaca
      781 tacatactat tgctgtcaac cgttccaccg tcagctagcc ccggaatccc aaaaagcgcc
      841 aaaataccat ttaaaaggag gttaccccat ccggaagtgg tctggccgat tccttgaaag
      901 atgttgccga taaaactggt gattcccgag aacgtcccgc taaatgattg gaaaaatcca
      961 ttcagaccgg attgcaaaat gttcgcggct tgtcccatcc aatcgggaag cccgcccagc
     1021 caagtatcaa aattctgctg taatccctgg aagagtgact gcccatcttg cgaaaaaacc
     1081 accttcaatg ctccatctag acctcccgat tgaaaggcat cgactacctg gccgatcaca
     1141 ttctgaatat ctccttgaat attcttgaaa tcgaagatct gtttgaaaag gtcgctcggc
     1201 aagtattgct ccaatacttt tcccagaccg ccttcttgaa agactgctgc aaatttattg
     1261 tactcatcct gtgcttttga aaactcctgt tgcagttggc caaagcgctg gtacatatct
     1321 tcgggcaata gctgctgaag tacgctgtcc cagttgcctt cctgaccggc ttgctgaatc
     1381 ttggcaatct gatctttaat cttctcaaaa ttcgttttga ccgtctcgaa ttgattaaaa
     1441 gtatccggcg ccagttcctt tagaaccttc tcccaatccc catcccggaa ggcctgagac
     1501 acttcatcgt attgggtctt aatctccgta aactgttctt tgagcttctg ataggtctcc
     1561 tccggaagca cttgctgtaa cgccttttcc agtccgcctt cctgaaaggc tgccgtgagt
     1621 ttgttatact cattttgagc ttttaagaac tcctgtcgga gctgatcaaa gcgttggtac
     1681 atatcttcgg gcaatagctg ctgaagtaca ctgtcccagt tgccttcctg accggcttgt
     1741 tgaatcttgg caatctgatc tttaagattc tcgaaattgg ttttagccga ttcaaattgc
     1801 tgataagtat ccggagccag ttcctttaaa gccttctccc agtctccatc ccggaaggct
     1861 tgcgacactt catcgtattt ggtcttgatc tccgtaaact gctctttaag ctttgaaagc
     1921 ttttgatagg tttcctccgg aagcaattgt tgcagtatgt tctccaggct tgtatcggta
     1981 aatgattttt ttaagtcttc aaaatagcca ttgatactct ggacggattc ctgcagtctt
     2041 ttaacggtat cctcaggaag caatttttca gtgagattaa ctaaaccgcc ttcctcataa
     2101 gcctgtttga gttcgttata ccggttattc agcttttccg tatactgctt gatttcatcc
     2161 ggtaaagtat tggcattttc caacgtttgg gcaacctgtt cccaaccatt tgtagaaatc
     2221 tcccgggcga tctccgagta tacggcgctt aacttggctg ttaaatcatt gccttctttg
     2281 gctatattgt ccggaataat tcgaatctga cctttaatgg cgctcgccaa aaggtcgaaa
     2341 ctttgggtca attgattcat gagcccattc atatcggttt gaatcgagct tccttcactc
     2401 atgctttccc tccttctgca atcgggctag ctcatataat tccagcattt ttaatttctg
     2461 aaataataaa ggggttattt caaccccaaa gagatcggca atctgaaaaa ccgccggata
     2521 atccagaccg atcggagcta taccgccaac ccgccattgg gtattgatca agcgccataa
     2581 gttccagact gcccggttcc cggggagtat ttcgggagat tgttccgggc actcggaaca
     2641 attggctaca gtagcggtct gttcttggtg cttccggcaa gcttggcaat attgactctt
     2701 gccctccggg gaccacgacc agaccgctag gagtttttta cctcaaccgc actcaaggtg
     2761 taggtgagct gatatgtttt ggtggctaac tccagacact ccggatacgg tacatttcca
     2821 aaattaaacc catgataaac atgatcaagg atccaatcca ccatttcggt attcaccttg
     2881 atggaaaccc cattttcatt gcaagccgga tcaagacctg cggtacgcaa ggccttcaac
     2941 tcatcacggg tcatcgcccg tggtttggtc agttccgtca ttttttaacc ctcctgtctc
     3001 ttttttgagt taataaataa tattaattcc accgggttat ttatctccag gtgcacccag
     3061 ggcatcctca tttaaaaaaa ccctccggat cggttgtgct ccgttaggtg tttcgaagac
     3121 cgcgaagcat aaccgaatcc atatagggtg tttgaagcgt aaatttattc caactgattc
     3181 tttaatactg ctttgttaaa atcgcttaaa tgaacgcttt gccatatcgt caatccgcgg
     3241 gataatcgtg tccagttgga cacgcgccca cggctgaacc caacccccat gaatggggct
     3301 tttaatcata gaatttaagc ccaaaggaat ttgatagccg gttttaaccg ggttgattta
     3361 aactagccgt ggggttcacc ccgcggtcgg cccgaaaagt gattaaacaa agtagaatta
     3421 atacataccc gattcaggtt taagttagtt cgttaagaac tacatcctca atacgctgcc
     3481 tggctattga ctaatgtcac cataatggca gtattttcag gattattgtt gcaataagcc
     3541 ctgaagggca gatcaattaa aatccctttg gctccttcga tcccgggaga attccgctcg
     3601 aaaacgattt ccggcaataa aagctccaaa ctatgttgca tattggtcag ctttagtgat
     3661 aacgaagact cggttccttg catcgcctta ttcagcaagg attgatcttc aaaaaacgcc
     3721 ttcagattcc cggtgatttt taacaacccc tccggaattc cggtacggca accattcccc
     3781 ccgatagcat aactatcgcc ggccagaccg aactccatat ttaggctgac attggatact
     3841 gtggcgatat tggcgccact ttccttaatc gaagcctgaa aattattgta tttggccaga
     3901 ctgatattag cgggtgaacc ggcgaacgac gcggatctaa ttgtctccat ggcacccatc
     3961 aggtcgatgc ttgcggtcag ctcggcatcg ccacccagag taagggagaa cttgtttact
     4021 ttacatccgt tgaataattc ataaacccca atatcggtaa atccttgctc cagtaccagg
     4081 gaaggttggg tatcacccgt tttgaaaacg tgcatataag gatccgcact accgctggtt
     4141 gcgggtgcac caaacatcgc cttcaaccaa tagccgatag caacctcatc aaccggcacc
     4201 actactgttc cagttagatc aatatttcct ttcgccggtt cggccggatc gcgacgaccg
     4261 gtaattacgc ttgagtcgat aaaattttgt ttggatttga gctgtgagga aacgatcggc
     4321 aatttgacag cattaaccgt tccgggagtt tgtccgaaga cggtttcaaa atcggctact
     4381 agctgtgctt tataaccctt tgcttgtgtc atgatttatg cctcctagtt ttattaggaa
     4441 aactgaactt cgtcctttca gggtaaatac atggactcat tcaattttca ttcttgcatt
     4501 aaagacctaa ttcgatgggc tttttcagga tcaataacta atactctccc ccatcgccgg
     4561 gaagatcgag accttgactt ccatccttcc ggtaaattgc gggaaggttt cttcccgtaa
     4621 cgtactgtaa ttcagatacg aaatgggatt tgagggactg gccgtcatca gcgcggttaa
     4681 aattagctgg ccaaaagcgt ccatatcata aagaccgtct aattcggtta cctgaccgga
     4741 cactgttttg gcagtattgc tgattgacca ttcgaccgga atcagatacc ggtattcctt
     4801 tctgttcatc ccctcggttt ttgaaccgga atgaatcaga atataggggc aatcactctg
     4861 agccggcagc gcattcggat ccaccccgat aaatattttg gggtgtgtcc cgtatttcgt
     4921 ctgacagaaa tcattgattg ccgggttgtt aagaagttca tcccgccatt tggtgataat
     4981 ctcgtgaatt gccaggctgg aaaacatcgt aacctccttt cccgtgactt ttcaaggtct
     5041 tctagatcta aacatgtaca gacagatcca aaccccgatc cggcacgtta ataatacaaa
     5101 cccaacaaca tttaggccga tggaatcccc aacctcaccg gaatcaataa ggctgccgga
     5161 ttctgacaaa acataaaccg cctcaatacc gagacggttt atgtttcatt gcgggacggt
     5221 cgatttcttg gtgaccggag cccttgatga aatgtttcaa aatttgtatg ccgcgcccca
     5281 ccctgcgctt aaatggcata ttaacccgtg gcatatcgcc acacaccaaa aaaaggtaac
     5341 aaaaaagcgc cctttgggga ggcgctttct aaatctctca tcgtgatcat tataatctat
     5401 aatggttcat ttgtaactgt caaaaaaccg ccggggaaaa attaatctag ctgcactttt
     5461 gtccgttttt actgtcggga atgtttagaa accattttgt ttttgccagg accgat
//
LOCUS       DASVMB010000509         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_359239,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000509 DASVMB010000000
VERSION     DASVMB010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359239"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF531_14290"
     CDS             <1..207
                     /locus_tag="VF531_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX7715181.1"
                     /translation="DTVTLRYQCDCSHDKFLGPLLSIGRQELQPLFQEEKEIEVRCHF
                     CNQTYYYTADQLKLLDEPGPQNAV"
     gene            255..761
                     /locus_tag="VF531_14295"
     CDS             255..761
                     /locus_tag="VF531_14295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEX7715182.1"
                     /translation="MLEILRPRVQAESVLELDLTELRNVGIRGIIFDLDNTLVEWKQD
                     TLDPEIMALVKRFKTQGFKMCILSNGLEHRVETIANTLGIPYVSRAVKPRKSPFKKAL
                     EILGTSPEETAVVGDQLFTDILGGNRMELYTIWTPPLSETEFVSTRAVRQIERLVLKR
                     FRKKGLLQ"
     gene            758..1894
                     /locus_tag="VF531_14300"
     CDS             758..1894
                     /locus_tag="VF531_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7715183.1"
                     /translation="MSDKYCRGCGSVLQSREPAAPGYVPETIGQSKERLICQRCYKMI
                     HYGQAGSLRPDPDLIRQHIRRAIGQSELLVLVADFADLTGTLPVWKDILGNKPYILVL
                     NKIDLLPSRTKLEEVLDYCKNYLTKWQFPHPEGIVAASGHKELGMAIISKLLKAGSGE
                     RTKIAFLGATNVGKSSLIKQMLQADGSPHTPTVSKFPGTTLALSNWDIFRGRNTLIDT
                     PGLVPEDRLIDKLCPECAGRLVNSTTEIAQKLWGLKPGKALLCGGLAGLINLGSASVR
                     PDPDNGENVFIGFTAPGFVMHRTDAERVDELLATGPQWLGEICTGCRQKLNWRVAEME
                     MEPGQDLAIAGMGWFSLRGRAARLQLTLPSGVRFEVRPALIGKR"
     gene            1975..2832
                     /gene="aroE"
                     /locus_tag="VF531_14305"
     CDS             1975..2832
                     /gene="aroE"
                     /locus_tag="VF531_14305"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7715184.1"
                     /translation="MVSFEINGNTGILAIFGHPVQHSLSPAMHNAVFYHLGWNYRYIP
                     CDVPSEQLSTAIDAIQALNFKGVNLTIPHKQAAINALDEIYGDSLESGSVNTIIRHHG
                     KLTGTSTDGIGFIRSLREEGRFDPTGKNVLLLGAGGTALAVVYRLIHAGIKRLTLINR
                     DCSKAQILGERIRKKTGFEPEVHGLDHLAALSGTEFDLVVNSTSVGLHDDQALLPKEF
                     LNSRQFVYDVVYRPGGTRLVNDARNTGCQVLSGLSLLIYQGAESFRLWFDTEPPLDIM
                     RNAVLGETQ"
     gene            2829..>3894
                     /locus_tag="VF531_14310"
     CDS             2829..>3894
                     /locus_tag="VF531_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715185.1"
                     /translation="MKRIPICFQSLVLRILAILFIATNSVWAADPVDPATVVNLEFYE
                     CELRQVFRDLAVNGGFQVVMDSTVAGPVSIAFRTRISAREAASITAKTYGLTLRWLPN
                     SDTAYIGKSIPENIGVKFASRTAKSFTLERASVTTVADALTVVLPQARIKTDLLNNQI
                     QVVASELELANVSELITRWDRITPYAYIETRIAEIPVPVWRELDLTPSGTAVIPAIYP
                     LNAAQSQKLLQNKTIWLARQSLTCLDYQTGRIFYGDALPQLTERSQSGEITYKVEYLE
                     VGTTLSLTPRIEHREQVGLKIEQKVMTITNQAKPGVRFNPITLSRDFTAFLSINPGQI
                     CILAGGLQRAEFDKLRNTPYQ"
BASE COUNT          978 a          929 c          996 g          991 t
ORIGIN      
        1 gataccgtaa ccctccgcta ccaatgcgac tgttcccacg ataaattcct cggccctctg
       61 ctcagtatcg gtaggcagga acttcaaccg cttttccaag aagaaaagga gattgaagtc
      121 cgttgccatt tttgtaatca gacatactat tataccgcgg atcaactaaa gcttctggac
      181 gagcctggtc ctcaaaatgc cgtttagatt caggcaactc gcgaaaaaaa ttaccatttg
      241 ggaggttagt gtggatgttg gagatattac gccccagggt tcaggcggaa tcggttctgg
      301 aactggatct gactgagctc cggaacgtcg gaatccgagg catcattttt gatcttgaca
      361 ataccttggt ggaatggaaa caagatacgc tcgatccgga aatcatggcg ctggttaagc
      421 gttttaaaac ccaaggtttt aagatgtgta ttctgtccaa tggtttagag caccgggtgg
      481 agaccattgc caacacttta ggaattccct atgtctcccg ggcagtcaag ccgcgtaagt
      541 cgccatttaa aaaggccttg gaaatcttag gaacatctcc ggaggaaaca gcagtggtgg
      601 gggatcaact cttcaccgac atcttggggg gcaaccggat ggagttatac accatctgga
      661 ctcccccctt aagcgaaact gaattcgtca gcactagagc ggtacgtcag attgagaggt
      721 tggttttaaa acgattccgc aagaaaggac ttttacagtg agcgataagt attgccgcgg
      781 ttgcggcagt gttttacaga gcagggaacc ggcagccccc gggtatgttc cggaaactat
      841 cggtcaaagc aaagaacgac tcatctgtca gcgctgctat aaaatgattc actatggaca
      901 agccggttcg ctccgacccg atccggactt aatccgccaa catattcgaa gggccatcgg
      961 ccaaagcgaa ctcctggtct tagtggcgga ttttgctgat cttaccggaa cgcttccggt
     1021 atggaaggat attttaggga ataaaccata catattggtg ctaaacaaga ttgatctcct
     1081 tccgtcccgg accaaattgg aagaggtcct tgattattgc aaaaattact tgacgaaatg
     1141 gcaattcccc catccggagg ggatcgttgc ggcaagtgga cataaggaat tgggcatggc
     1201 cattatttcc aagcttctca aagccggaag cggggaacgg actaaaatcg ctttcctcgg
     1261 cgcaacgaac gtcggcaaat cctccctgat caaacagatg cttcaagccg atggctctcc
     1321 ccataccccc acagtctcta aatttcctgg aaccactttg gccttgagca attgggatat
     1381 cttccgcggg cggaacactc tcatcgatac tcccgggctg gttcccgaag accggctcat
     1441 tgataagtta tgtcccgaat gtgccggccg cttggtcaat agtactacgg agattgctca
     1501 aaaattatgg ggtctgaaac ccggtaaggc actgctatgc ggggggctgg cgggtttgat
     1561 caatcttggg tccgcaagcg tccgtcctga tcccgataat ggtgaaaatg tgtttatcgg
     1621 gtttactgca ccgggattcg ttatgcatcg taccgacgca gaacgggtcg atgaattgct
     1681 ggcaaccggg ccccagtggt tgggtgagat ctgtaccggg tgccgccaga aattgaactg
     1741 gcgagtcgca gagatggaaa tggaacccgg tcaagatctg gcgattgccg gaatgggttg
     1801 gttttcactg cgggggagag ccgcgcgctt acagttgact ttgccttcag gagttcgttt
     1861 tgaggtccgg ccggcattaa tcggcaaacg ttgatttccc aaggccatag caccagcgat
     1921 aattatcctt cgcaagcggc aggagatcgc tattcttgaa gcgaattagc tagtatggtt
     1981 tcttttgaaa taaatggcaa tacaggaatt ctggctattt tcggccaccc cgtgcagcat
     2041 agtctttctc cagctatgca taacgcggtt ttttatcatt tgggctggaa ctatcgatat
     2101 attccctgcg atgtcccatc ggaacaatta tctacagcga tcgatgcgat tcaggcctta
     2161 aatttcaaag gggttaatct caccatccct cataaacaag cggcaataaa tgctcttgat
     2221 gaaatttacg gtgattccct ggaaagcggc tcggtcaata caatcatccg gcatcatggg
     2281 aaactcactg gtaccagcac tgatggaatc ggtttcatcc gctctttacg ggaggaaggc
     2341 cgttttgatc cgaccggcaa gaacgttctt ttgttgggag ccggtggtac tgcgttggcc
     2401 gtggtttacc gtttaattca tgcggggata aaaagattga ccctgataaa tcgggattgc
     2461 tccaaggcgc aaatattagg ggagaggatc cggaaaaaga ccggtttcga acccgaggta
     2521 catggactgg atcatctagc ggcgctttcc ggaaccgaat ttgatctggt ggtcaattca
     2581 acctcagtgg gattgcatga tgatcaggcc ttgctgccca aggagtttct caattctagg
     2641 caattcgtat atgatgtggt ttaccggccg ggagggaccc ggcttgtaaa tgacgcccgg
     2701 aacacaggct gtcaagttct ttctggcttg tcattactga tttatcaggg tgcagagagt
     2761 ttccggttat ggtttgatac cgaaccgcct ctcgacatta tgcggaatgc agttttggga
     2821 gaaacgcaat gaaacgaata ccaatatgtt ttcaatctct cgtcttgcgg attctagcca
     2881 ttcttttcat cgccacgaat tcggtttggg cggcagatcc ggtcgatccc gccacagttg
     2941 taaatttgga attttatgaa tgtgaattac gccaagtatt ccgggatctt gcggtgaacg
     3001 gcggttttca agtggtgatg gattctaccg tagccggtcc ggtgtcgatc gctttcagga
     3061 cccggatctc tgcccgggag gccgcgtcga ttactgccaa aacctatgga ttgaccctac
     3121 gttggctacc caattcggat acagcctata tcggaaaaag tatccccgag aatatcgggg
     3181 tcaagtttgc atccagaacc gcgaaaagtt ttactttgga acgggcttcg gtcacgaccg
     3241 tagcggatgc tctgaccgtg gttctgcccc aagcgcgcat aaaaacggac ctgctgaata
     3301 atcaaatcca agtcgtggca agtgaacttg agctggcaaa cgtttctgaa ttgattaccc
     3361 gttgggatcg gattactccc tatgcttata ttgaaacccg gatcgccgag atccccgttc
     3421 cggtctggag ggaattggac cttactcctt ccggaactgc cgttattccg gccatttatc
     3481 ctctaaatgc cgcacagtct cagaaattac tccagaataa aacgatctgg cttgcccggc
     3541 agagcttgac ctgccttgat tatcaaaccg gaaggatttt ttacggggat gccctacccc
     3601 aactcaccga acgctctcag agcggggaaa tcacctataa agtcgaatat cttgaggtag
     3661 gaaccacctt aagcctaacg ccccggatcg agcatcgcga gcaagttggg ttaaagatcg
     3721 aacaaaaagt tatgaccatt acgaaccagg ccaaaccggg ggttcgtttt aatccgatca
     3781 ctctaagccg ggattttacg gcatttttat ctataaatcc tggacaaatt tgtatcctgg
     3841 ccggaggttt acagcgtgcg gagtttgata aattgcgaaa tacgccttat cagt
//
LOCUS       DASVMB010000510         8175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_208613,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000510 DASVMB010000000
VERSION     DASVMB010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208613"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF531_14315"
     CDS             complement(<1..849)
                     /locus_tag="VF531_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7715186.1"
                     /translation="MSLSRNQSRPILLSIIFMVATVIFWGYSFISTKIVLTEEPPVSI
                     AFFRQIIAALILIPWVMATKSHIRVSLKDMGLILASALFGIVLYFICENNGLRYTTAA
                     NASMIVAAVPIFTLFSESLFFKMKITPRMVICILLSILGVYLIISSNGRLDFSSAAFR
                     GNLLVIGAMVCWVIYTIINKGLSSRYSSLQLIMDQSIGSIFLFIPFILPEAHYWQRLT
                     WVPFFNLLYLGVFCSAISYFFFIYAVKRLGATVSSAFLNLIPVVTVISGFFVLGEKLI
                     WLQILGM"
     gene            complement(892..1218)
                     /locus_tag="VF531_14320"
     CDS             complement(892..1218)
                     /locus_tag="VF531_14320"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX7715187.1"
                     /translation="MARPSNTALFKLASWFVPPLVLMVIPTATLETGPVLCLFRILWG
                     IRCPGCGMTRALSCVLHAHFSQAFYYNSLVVLVFPLLVLFWWRGVKRGFRRWRDLVEY
                     ERRVSD"
     gene            complement(1224..1595)
                     /locus_tag="VF531_14325"
     CDS             complement(1224..1595)
                     /locus_tag="VF531_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX7715188.1"
                     /translation="MYCRNCGKEVAEQAVYCVACGVPPKKGSKFCQNCGAESDSQAVV
                     CTKCGVRLVRGEAKDWLTTLLLAIFLGGLGVHRFYTGHIGTGVLQLLTLGGCGIWALI
                     DIILIATDSFKDYEGNPLTKK"
     gene            1896..2171
                     /locus_tag="VF531_14330"
     CDS             1896..2171
                     /locus_tag="VF531_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7715189.1"
                     /translation="MTPSATKKLTTVAIHDTGKGIPKKNLPRVFEPFFTTKGPDDKHQ
                     NYGLGLSYCQTIVKKHGGKIDITSEENRDTSVFLSLPSAKAGVKSNI"
     gene            2288..3325
                     /locus_tag="VF531_14335"
     CDS             2288..3325
                     /locus_tag="VF531_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX7715190.1"
                     /translation="MYPADLGLGMPPEWNSHARTLMEWPVLEAEWPEPFSEILPAYVE
                     ILRAITFFEPVTLVVRPDRVAEATAHCGPGVSILPLEHNDSWMRDNGPTFVKNNSGEI
                     AGINWIFNAWGGKFPFEMDNQVAPEVLRNMGIPCFNAPIVLEGGSIHVDGAGNLLTTE
                     SCLLNPNRNPGLSREKIETLLRSYLQVRQIIWLPQGWTGDDTDGHIDNVACFAAPGCI
                     LTQVCRDPADPNYEISQLNLEILNAATDNQGKPFRVIPIEQPPAAYYQDVRLTLSYLN
                     FYFVNGGIVMPVFGGAARATDQAAIATMQQVFPDRRIVPVNGSIIARGGGNIHCLTQQ
                     VPTGTPYSNQP"
     gene            3358..4245
                     /gene="aguB"
                     /locus_tag="VF531_14340"
     CDS             3358..4245
                     /gene="aguB"
                     /locus_tag="VF531_14340"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX7715191.1"
                     /translation="MRQVTVAAIQMACSRRLEQNLETAERLVREAAARGAQIVLLQEL
                     FATPYFCQEESTAYFEWATVPEASPAVLCCQALARELRLVLPVSFFERSRNIFYNSAA
                     IIDADGKMLGIYRKTHIPCGPGYEEKFYFHPGDTGFQVWQTRYARIGVGICWDQWFPE
                     TARCMALQGAELLLYPTAIGSEPAEADIDSKFHWQTCMQGHAAANIIPVIAANRIGTE
                     TVRGSSITFYGSSFITDHTGAKLTEADRSSETVLTASFDLDAIEKQRISWGVFRDRRP
                     EMYRLLLTADGVPARDSKL"
     gene            complement(4379..4981)
                     /locus_tag="VF531_14345"
     CDS             complement(4379..4981)
                     /locus_tag="VF531_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715192.1"
                     /translation="MRKLSVLVVAVLLLMLAAWPAYADETLLSGDVDNGGFGGPGMKF
                     TQVNGTAGTLVGGSGGWLIDHQLLIGGGGYGLAGKIKAPASAQPSDTTLYINMGYGGL
                     VMEYTPNSGKLYHLDYSLLIGGGYVSYAKSNYDPNFDQPGTTFFVAEPGVRLTLNVSD
                     YFRAGVGASYRYVNGVELTGLDSAALSGVSANLFFTFGQF"
     gene            complement(5032..6207)
                     /locus_tag="VF531_14350"
     CDS             complement(5032..6207)
                     /locus_tag="VF531_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7715193.1"
                     /translation="MDIFEKCFNFTAAKEVMQAGIYPYFHELETKQDTEVVINGKRTI
                     MIGSNNYLGLTSDPRVIKAAMEATEKYGSGCTGSRLLNGTLKLHVELEKRLAEFLQKD
                     NTLTFSTGFQTNLGIISGLAGRNDYIIGDKKNHASIVDACRLSFAKFLKYEHNDMEDL
                     ERILKSVPETAGKLIVTDGVFSVEGEICKLPEIVKLARKYGARIMVDDAHGLGVLGKH
                     GRGTGEYFGLEDEVDVIMVTFSKSLASLGGCVAAREEVIHYLKHNSRPFLFSASIPPA
                     NAAAALKALEILESEPERITRLAENANFMRQGFKKLGLNIVDAPTPIIPVLTYEDEAT
                     FVYTKRLLEEGVYVNPFVSPGTPPGQAILRTSYTATHTREQLEYALKAFEKVFGVKK"
     gene            6494..7327
                     /locus_tag="VF531_14355"
     CDS             6494..7327
                     /locus_tag="VF531_14355"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7715194.1"
                     /translation="MNTVYGEVILITGASSGIGKTLANFLMSKGYKVYGTTRQPVTQE
                     AELFQTSQNGGFLRMIQLDVRSDESIRKALEYIIGREGALHVLINNAGYGIAGSVEDT
                     TAAEAQQQFDTNFFGVLRMCNQVVPIMRRQHKGLIINVSSVAGLISIPFQSMYSASKY
                     AVEALTEALRMEVKPFGIRVTMVEPGDTKTGFTANRQAVAAAGDSIYKERFTRSIQEM
                     ARSEQNGPEPKTILRVVAKMLRRKNPPIRTVIGGSYKIIAFLKRILPSRCVEFVVSKV
                     Y"
     gene            complement(7460..>8175)
                     /locus_tag="VF531_14360"
     CDS             complement(7460..>8175)
                     /locus_tag="VF531_14360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7715195.1"
                     /translation="EQTICVSKDEYRKLKTAGIKSTNLNAIYNGVPDQEFSTDEAHRK
                     LSLSPNLPVIGCVARLEQPKGIEYLIKACGILIERGIAVQLVLIGWGSEEYRLRKIAS
                     EYPNLPVVFTGALEETGPYYKLFNLYVQPSLVEPLGITAIEAMRAGLPVIASQVGGLA
                     ELVIDNETGVLIPPGNADAISEAIRKLLEDQALAQKMGQAGRKRFLNYFMVEQTTRKT
                     VEVYEKALKYSDGITIFPG"
BASE COUNT         2227 a         2114 c         1836 g         1998 t
ORIGIN      
        1 cattccgagg atctgtaacc atatcagctt ctccccgagg acaaaaaaac ctgagattac
       61 ggtgactacc gggatgaggt taagaaacgc cgatgatacg gtggcaccca accttttgac
      121 cgcgtagata aagaaaaaat aactaattgc cgagcagaac actccgagat agagcaagtt
      181 aaaaaaaggc acccatgtta accgttgcca ataatgggct tccggaagaa tgaacgggat
      241 aaacaaaaag atactcccga tagactggtc catgatcaat tgcaggctgg aatagcggct
      301 gctcaaccct ttattgatga tggtgtagat gacccagcat accatcgccc cgatgacgag
      361 tagattcccc cggaacgcag ccgatgagaa atccaatctg ccgttgctgg aaataatcaa
      421 atacacccca agaatggaca aaagtatgca tattaccatt cgtggtgtga ttttcatttt
      481 aaaaaagagt gattcgctaa ataaggtgaa gatcggcacc gcggcaacga tcatcgaggc
      541 attggcggca gtggtgtagc gaagcccgtt gttttcgcag atgaaataga gtacgatccc
      601 gaatagtgcg gaagccaaga ttaaccccat atctttgagg cttacccgaa tgtgggattt
      661 ggtagccata acccaaggaa ttaagattaa ggcagcgatg atctgccgaa aaaaagcgat
      721 ggataccggc ggttcctcgg tcaagacgat tttcgtgctg ataaaggagt atccccaaaa
      781 gatcactgtt gccaccatga atataattga cagaaggatc gggcgggact ggttccgcga
      841 taatgacaag ttaatctgct ccttgatgat aatttaaaat ggaatgttga ttcaatcaga
      901 gacccggcgt tcatattcca ccaaatcccg ccaacgtcta aaaccccgtt tgactccccg
      961 ccaccagaaa agaactaata gcggaaagac caggactacc aaggaattgt aataaaaagc
     1021 ctggctgaaa tgggcatgaa gaacacaaga taacgccctg gtcatgccgc aaccagggca
     1081 acggataccc cagaggattc gaaacaggca aagaacaggc cctgtttcta atgtggcagt
     1141 gggtatcacc atcaaaacca gcggcggaac aaaccagctc gcaagtttaa ataaagcggt
     1201 attcgaaggt cgtgccatct acgttacttt ttggttaagg gattaccttc ataatcctta
     1261 aaagagtccg tggcaatcag gataatatca atcagcgccc agattccgca gccgcccaaa
     1321 gtcaacaact gcaacacgcc ggttccaata tggccggtat aaaaacggtg tacacccaat
     1381 cccccgagga atatcgcaag caacaaggtg gttaaccagt ccttggcctc gccccggact
     1441 aaccggactc cgcacttggt gcagaccacc gcttgagagt cactctcggc gccgcaattc
     1501 tggcaaaatt tcgaaccttt tttcggcgga acaccgcaag ccacacagta aaccgcctgt
     1561 tctgcaactt cttttccgca atttctacaa tacattccct aaccccccac ccacatactc
     1621 aaatacccaa cttacgcttc aacatcattc caaaggtgct gccggatcaa taaacgacat
     1681 attacttggt tctatcttta tatgtattat agcatagagt tatgcaatag catatcaaag
     1741 cctgacaaaa ataactttaa cattccccct caagaaacat caaggaaacc cgttttttta
     1801 gcgaatgaaa tatacgatat tatctttgaa ggaagtcttg atcaatatct taaaaaacgc
     1861 ggttgaggca atggaaaacg ggggaaactt aaccgttgac accctctgca acaaaaaaac
     1921 taacgacagt cgcaatccat gataccggca aaggaatccc caaaaagaac ctgccacgag
     1981 tcttcgaacc attcttcacc accaaaggtc cggatgataa acaccaaaat tacgggcttg
     2041 gcctttcata ttgccaaact attgtgaaga aacatggcgg caagattgat atcaccagtg
     2101 aagaaaatcg ggatacctca gttttccttt ctctccccag tgcgaaagct ggcgtcaaat
     2161 caaatatttg aacaacctga atccaaaata ttccgactac ctatgaactt acagccaacg
     2221 ctttgtaatc accctgattt atgttacaat agacaatgcg aaattttatg ttgaggtgta
     2281 cagaacgatg tatccagcag atttaggttt ggggatgccg ccggaatgga atagccacgc
     2341 ccggacctta atggaatggc ctgttttaga ggccgaatgg cctgagccgt ttagcgaaat
     2401 tctgcctgca tatgtggaga tcctccgggc gattactttt tttgagccgg taacgctagt
     2461 agtccggccg gatcgcgtgg cggaagcaac cgcgcattgc ggtccgggag tatcgattct
     2521 tcctctcgaa cataacgatt cctggatgcg ggataatggc ccgaccttcg tgaagaataa
     2581 ctccggtgaa atcgcgggga tcaattggat ctttaacgcc tggggcggaa aatttccatt
     2641 cgaaatggat aaccaagttg ccccggaagt cttgcgtaat atggggatcc cttgtttcaa
     2701 cgcgcccatc gttttagagg gcggttcgat ccatgtggac ggcgccggaa acctgctaac
     2761 cactgaaagc tgccttttaa atcccaatcg caatcccggc ctatcacggg aaaagattga
     2821 aaccttatta agatcctatc tccaagtaag acagattatc tggctacccc agggttggac
     2881 cggagatgat accgacggcc atatcgacaa tgtggcatgt tttgccgcgc cgggttgcat
     2941 cttgacccaa gtctgccgcg atccggccga tcccaattat gaaatatccc aactaaactt
     3001 ggagattcta aatgcggcta ccgacaatca gggaaaacct tttcgggtga ttccgatcga
     3061 acaaccaccg gccgcatatt accaggatgt ccgcctgact ctcagctatc tcaattttta
     3121 ctttgttaac ggtggaatcg tgatgccggt ttttggcggc gccgcacgag ccaccgacca
     3181 ggcggcaatc gctaccatgc aacaggtctt tccagatcgg cgcatcgttc cggtaaacgg
     3241 cagcatcatt gcccggggcg ggggcaatat ccattgcctt acccagcaag tgcctaccgg
     3301 tacaccttac tccaatcagc cttaattgtt tctaaaaccg aagggaggga ttcgttcatg
     3361 cgtcaagtta ctgtggctgc aattcaaatg gcctgttccc ggaggcttga gcaaaatctt
     3421 gaaaccgccg aacgcctggt tcgtgaagcg gcggcccggg gagcacagat cgtattgctt
     3481 caagagcttt ttgccacacc ctatttctgc caggaggagt ccaccgccta ctttgaatgg
     3541 gccaccgtac ccgaagcaag tccggccgtg ctatgttgtc aggctttagc ccgggaactt
     3601 cggttggtgc tccccgtaag tttcttcgag cggagccgga atatctttta caattcggcg
     3661 gctatcatcg acgccgatgg aaagatgctg ggaatctatc gcaaaaccca tattccctgc
     3721 ggtcccggat atgaagaaaa attttatttt caccccgggg ataccggttt tcaagtctgg
     3781 caaacccgtt atgcccggat cggagtggga atctgctggg accagtggtt cccggaaacc
     3841 gcccgttgca tggcgttaca aggggccgaa ctattactgt atcctacagc catcggttcc
     3901 gaacctgccg aggccgatat cgattccaaa tttcattggc agacttgcat gcaaggccat
     3961 gcagcggcaa acattatacc ggtcatcgct gccaaccgga tcggcaccga aactgtcagg
     4021 ggctccagta tcacctttta cggctcctct tttatcaccg atcataccgg cgccaaatta
     4081 accgaagcgg accggagttc cgagaccgtt ctcaccgcca gcttcgattt ggatgccatt
     4141 gagaagcagc gaatctcctg gggagtcttc cgggatcgcc ggccggaaat gtaccggctg
     4201 ctcttgacgg cggacggcgt tccagcccgg gactccaagc tttaaacgga tcgtggatat
     4261 gaatcccatg ataaatcaaa ggcgtaccgg aaaaggtacg ccttatcatt ttaatcgggt
     4321 tatttatgat aacgaatatt ttggccaatg atacgggaac acgtcatcgg catattgatt
     4381 agaactggcc aaacgtgaag aacagattgg cgcttacacc gctcaaagcc gcgctatcca
     4441 acccggtcaa ttcaacaccg ttgacataac ggtaactggc tcccactccc gcccggaaat
     4501 aatccgaaac gttcagagta agtcttactc cgggctcagc cacaaaaaag gtggttcccg
     4561 gttgatcaaa attcggatcg tagttactct tggcgtaact tacatagcct ccgccgatca
     4621 agaggctgta atccaaatga taaagcttgc cggagttcgg ggtatactcc atcaccagcc
     4681 cgccataacc catattgata tacagtgtag tatcggaagg ttgggccgaa gccggagctt
     4741 taatcttccc ggccaacccg taaccgccgc cgccaatcaa caattggtgg tcaatcaacc
     4801 agccgccgga accgcccacc agtgtgcctg cggtcccatt cacctgggta aatttcatcc
     4861 ccggtccgcc gaaacctccg ttatcgacat ccccgctcag caaggtctca tccgcataag
     4921 ccggccacgc cgccagcatt aatagtaaca ccgcaaccac cagaacgctt aatttacgca
     4981 tcgtaacctc ccaaaatgaa attgcttact tttctcagca gttcccaccc atcacttctt
     5041 tacaccaaat accttttcga aagctttaag ggcatactcc aattgttccc gagtgtgggt
     5101 cgccgtataa ctagtccgta agattgcctg tcctggcggg gttccgggcg atacaaacgg
     5161 attgacataa acgccttctt ccaagaggcg cttggtgtaa acaaatgtcg cctcatcttc
     5221 atatgtcaat accggaatga tcggcgttgg agcgtcaacg atattcaaac caagcttttt
     5281 aaagccttga cgcatgaaat tagcgttctc cgccagccga gtgattcgtt ccggttccga
     5341 ttccaatatc tccaaggcct ttaaggccgc agcggcgttc gccggtggga tcgaggcgct
     5401 aaagaggaac ggacgggaat tatgtttcag gtagtggatt acctcttcac gcgcggcaac
     5461 gcatccgccc agactagcca gggatttact gaatgtcacc atgatgacat ccacttcatc
     5521 ctcaagccca aaatattctc cggtgccccg accgtgtttg cccaatactc ccaaaccatg
     5581 agcatcgtca accataatcc gggctccata cttgcgggct agttttacaa tctcgggcaa
     5641 tttgcagatc tcgccttcca cgctgaagac accgtcggta actatcagct tcccggcggt
     5701 ctcgggaacc gatttcaaaa tccgctccaa atcctccata tcgttatgtt catatttgag
     5761 gaacttcgca aaactgagcc ggcaagcatc gacgatactg gcgtgattct ttttatcacc
     5821 gataatgtaa tcgttccggc cggccaaacc ggagatgatc cccagattgg tctggaatcc
     5881 ggtgctgaag gtaagggtat tatccttctg caggaactcg gccaaccgtt tttccaattc
     5941 cacatggagc ttcaatgtac cgttcagcag gcgtgagccg gtgcatcccg aaccatactt
     6001 ttccgtggct tccatggcgg ctttgattac ccgcgggtct gaagtcagtc ccaaataatt
     6061 gttggatccg atcataatgg tccgtttgcc gttgatcacg acttccgtat cctgtttggt
     6121 ttcaagttca tgaaaataag gataaattcc agcctgcata acctctttgg ctgcagtaaa
     6181 attaaaacac ttttcaaaga tatccatcga gtagaaaact cctccttaaa tattccctta
     6241 ttctatattt ttacgttgat cgtgataaga atcgttttgg tgatgatgca aacgaaccaa
     6301 tcctgtcttg aacttgcttc atcaaaaaat gctaaaaata ttgtatcaca gaattacact
     6361 ttactaccag tttttatatc gttttcaata ataaaaccgg actctggtat ctcaacatgc
     6421 aggatgattg gaattcgtag cgaataaatt tgcaatcatc gaaaatctaa atccgcggta
     6481 aaaggagtct gccatgaaca cggtttacgg agaagtaatc ttaatcaccg gtgcttcttc
     6541 aggtattggg aaaacattgg cgaacttttt aatgtccaaa ggttataagg tttacggcac
     6601 gacccgacaa ccggttactc aggaagcgga actgtttcaa acgagccaaa acggcggttt
     6661 tcttagaatg atccaacttg acgtccgctc cgacgaatca atccgtaagg cacttgaata
     6721 catcatcgga cgggaagggg cgctgcatgt tttgatcaac aatgccggtt acggtatcgc
     6781 aggctccgtg gaagatacca ccgccgccga agcccaacaa cagttcgata ccaatttctt
     6841 cggcgtgctg cgcatgtgca accaggtggt tccgattatg cgccgccaac acaaaggatt
     6901 gatcatcaat gtcagctctg tagcgggcct catctcgatt ccgtttcaat cgatgtacag
     6961 cgccagcaaa tatgcggtcg aagcattgac tgaagctttg cgcatggagg taaaaccctt
     7021 cggaatccgg gtaaccatgg tagaacccgg cgacacgaaa accggcttta ccgccaaccg
     7081 ccaagccgtg gccgcagcgg gggattccat ttataaggag cgtttcaccc gctccattca
     7141 agagatggcc cgttcggaac agaacggacc ggaaccaaaa accatcctca gggtcgtcgc
     7201 aaaaatgctg cggcggaaaa atccgccgat ccgcacggtt attggcggtt cttacaaaat
     7261 cattgctttt ttgaaacgga tccttccttc ccgttgcgtg gagttcgtgg tttccaaggt
     7321 ctattaacca ttccaatatt gttaaaccat tactccgccg gcttccggaa tggttgatta
     7381 tacatataaa ttaattgcaa cgcaccgtga atatggataa ggtcgatatc aagcggtttc
     7441 tagttaccaa agaaaccctt caacccggaa aaattgttat tccatcggag tattttaaag
     7501 ctttttcata aacctccact gttttcctgg tggtttgttc caccataaaa tagtttaaaa
     7561 agcgtttccg accggcttgg cccatttttt gagcgagagc ttgatcttcc aacagctttc
     7621 gaattgcctc tgaaattgca tccgcattcc ccggtgggat taaaacgccg gtttcattat
     7681 caataactaa ttcagcgagt ccccccactt ggctggcgat taccggcaag ccggctcgca
     7741 tcgcttcaat tgccgtaatt cctaacggtt cgacgagtga aggttgaacg taaaggttaa
     7801 agagtttata ataaggaccg gtttcttcca aagctccggt aaacaccacg gggagattcg
     7861 ggtattcact ggcaattttc ctcaaccggt attcttcgct gccccaacca attaacacca
     7921 actggactgc aattcccctt tcaatcaaga ttccgcaagc cttgattaaa tactcaattc
     7981 ccttgggctg ttccagccta gccacacaac cgatcaccgg tagatttggg gacagtgata
     8041 attttcgatg agcctcatcg gttgaaaact cttgatcagg aaccccgtta taaatggcgt
     8101 ttaaatttgt gcttttaatg ccggctgtct taagttttct gtattcatcc ttggaaacac
     8161 aaatcgtttg ttctg
//
LOCUS       DASVMB010000511        21152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_502634,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000511 DASVMB010000000
VERSION     DASVMB010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 21152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21152
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502634"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="VF531_14365"
     CDS             complement(<1..2154)
                     /locus_tag="VF531_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715196.1"
                     /translation="MAKKYVMHVICGTHWDREWRHTAEQSKLRLVDLMDSIIELLEQN
                     QEYRHFCVDGGTVVLEDYLTIRPENKDRIRKLIETGKMQIVNWYTLPETNTVAPEALI
                     RNLLLGRKMAWEFGGSMRSGYTATSYGQNSQMPQIYQGFGIDTAIFYRGTNKHILAPL
                     YRWEGADGSKLYALRTFDEVTRTNWYFYIHQPLVLGKPCRDLTYTYDRNHDPVHLCDE
                     GLYEKAFTLLREDSNFRRDPDSLEQAMRGILNQAMPYAIGNQLLALNMEDNDQPFPLL
                     PEMIQALNQISPDVEIIQTTLDEYMNTIIRQSDEVNLPVHKGELRYTTVEHGSFNALL
                     GATHSSRIKLKLLNDRVETNLLYLAEPLATLAAVYGKEYPKINLNRAWEALLKNHAHD
                     SICGAAVDRAHEDMLYNFSIANTVAEEVAGRSVTALFRRINTAKCFNRNDHTITLFNT
                     LGVPRQGVVPVVIDLPEKAKDSGAGSIGVDGANTGDVFYDIVDAEGRPIEYVELSSEP
                     VKIGVERELDCKAIKFAANRRRILIHAAVPAIGYSTYALRLRKPNYIFQPQEGLDRKL
                     IARENGVLENEHLKVIIHSNGTFSLLHKASGRIMDNLHYFTDSGETGSAHISNQPQRN
                     PIITSLGNAAAITMIESNRLRGVYKIEMKLEIPAAATLDSRDRFRCTVELPITYWLTL
                     EKDCPYLKIRTRLRNEARDHKLCVNFPSGVHTDMVV"
     gene            complement(2372..3709)
                     /locus_tag="VF531_14370"
     CDS             complement(2372..3709)
                     /locus_tag="VF531_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7715197.1"
                     /translation="MKKYLGLLLAVALLLAMMIPAVSGADYKEFHSLNLGGIVGKAIE
                     EIPKEQARQDKLDPDKKDAIGRMPLETEIAIEKFLNKDGYTFVTDDWGWAEPLVQKQV
                     AAFVAKDSEVNIFVGETQQPGFAMKGYYEPFPAALAAKIRKNVVPAAWKPMEYKGKIY
                     GVAGLPGVNAFYWNKDLFKKAGLDPEKPPKTWNQILEYAEKIDKAGNGQFYGGGVYMG
                     PNYGGSLRYTPFVLMLGGGYVDSKGYPTLDKAANVTAFEYLRKLSKHLPPGIAAAPSE
                     GAFWDAMNQQRFAMLIDGPWRVAEGARAGLDFGVSTIPLPDKGGKAANVTIGASFAAV
                     PKYAKYKDEAFKLIEFITMDKGFQTLQASTGQRPSVLASVSEDPAYLAKYPFMKAIFD
                     AEKGNVQGLPTFAKNDVKVWDVLNRAVSKIVITNQPVKDILKQAQKEAMALMK"
     gene            complement(3842..4861)
                     /locus_tag="VF531_14375"
     CDS             complement(3842..4861)
                     /locus_tag="VF531_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715198.1"
                     /translation="MAMNSNATQLQTAPQRKKRAGNWLTHLILALGGAVLIYPLIFSF
                     LVGFFTTYEFATRKSDTLFPWPQHPTLRNYTMFFTSDKTIFDLPSEEATKGRIRIDTA
                     SVGSVLNYGPQILTNSVLRTVYMTVLAVLTSLLGGYAFSRLKWKGRDKVFLLLLTTQM
                     VPGVVSFIPQFVELHRWPLAGGNNIFGMGGHGLYNTFGVYLFTFCALNVMGTFLVRQS
                     LLRTPMELDEAARVDGASTFRIIFSLLAPLQKPVLAYIAITTAIGNWNDWMVPFFFTN
                     DERLLTLPGGITRIIAMFTVKQDTPNYPMIITLGMALTIPSLIILALFQKYMVEGLAN
                     TGIKG"
     gene            complement(4864..5802)
                     /locus_tag="VF531_14380"
     CDS             complement(4864..5802)
                     /locus_tag="VF531_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715199.1"
                     /translation="MNTTNAGIQRLTEPRINPFARFYRKHPDGVRAAIILVPLLGWWF
                     VVSGGPMFFGLLLGFFDWHNVLETPKWNGIQNFFTFFGDPNYRLTLWNSIWIGLLTAA
                     VTNLLGLGAALLMNMPIRGRGFYRTIWYVPAVTSAAATTQIFNMWLEPSQIGLVNNII
                     QKFGAQPVIWQYSTGWMVFWIVFYTVWKGLGVSAILWLAGLQSVNPQLFEAAKIDGAG
                     NWQCFRHVTIPGLRPFITFIIINACIAAIQIYEQVLFISGGGPFGSTNVLMSQIYKDA
                     FVDFNLGMAGAGSMILAAVVFGLVLVNWVTRDKEEE"
     gene            complement(5857..7104)
                     /locus_tag="VF531_14385"
     CDS             complement(5857..7104)
                     /locus_tag="VF531_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7715200.1"
                     /translation="MEQKRTQLSQKTAETVNKEIAMPLYSQIKELIMRSITSGEFKAG
                     SRIASEVELAQKYGVSRITTKQALLELMQEGIVYRVPGKGTFVKSLPSQPEAGLANNN
                     SRPYEQFEAVTSENGKIYPTGTANVGLILPYLNDSYEAGIVSGAERELRNLNYHLVFR
                     KSDNSMAEENRAIETLRAEGIQGLIIFPIEGAYCSNALIKLKVDGFPFVLVDRFFRGI
                     DTNYVVSDNFGGAYAAGKYLIDLGHRRIGLVGYSPENVISINSRIRGFTQALADMGIT
                     LPGQRMELTLEREQVYNHQIGRADPEAVRTIQGYLKKNPDLTAIFAINDYTAICVLRA
                     ALQLGIRVPEDLNIIGFDNNLLSANVEVPLTTVNQPKKEIGQNAAQLLLKSIRNPAAD
                     TEKVILPAEMVVRASTCPPDPKT"
     gene            7469..7798
                     /locus_tag="VF531_14390"
     CDS             7469..7798
                     /locus_tag="VF531_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715201.1"
                     /translation="MNSMRLETCTLMCNEMLTTVGQAIGIHVLLLVIEHSLWLAGQQY
                     KEAALIRFSEQGVFLDRLNDIEPEKAIAVAYEFITAIMATLGKLMGKQLAKQFTEELQ
                     IERIQLK"
     gene            7853..10036
                     /locus_tag="VF531_14395"
     CDS             7853..10036
                     /locus_tag="VF531_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7715202.1"
                     /translation="MKLNHLVNSRRWVDFLESTAMGLQLNLALRSLTEPAIGLQAPGV
                     CPDCNRVYPRLTVEDLAAVKTFHPRSPHQNEEGHEFVTRNSEPFFSLPVQDGLFLIAR
                     ECPCKTATGLLSLSQRAKVAQKLLSAFLDTLSEGIAGSHAALEFSALRQINQILLSLF
                     RGESGASRRAFDLILSALIIMLDADASWLEYRDETGEQRIFKGEPAAIASHLRMKVPG
                     EILQIEVEGAYTKGSLSLLRPGDPKLAASLLPLMAQECSIVFEVEHLFRLMQSQLSRL
                     LGALNSAVFLVNEHMQIAYLNPAAEALLHQSAFSLIGISIGVLPQFWADRISNQVDEP
                     IRDYKTTFHRNTDSILLDWQILPLRDDTLVAGWLIIADDRTDAYHWQEIGRQAESFTT
                     VASVISPLAHELRNPLAAAKGLLQLVQRHNDPDKVSGYINLILREIDRMSILVNEFLQ
                     IGRSTGGHFESVTLSSLVEDLLPLVEGESAAGAVEIRCNLEPVPSVRADPGQLIQVLL
                     NLIHNAVEAAGPEGRVEIDLKSQGDWIVLEVSDNGPGIEAEVFGKLFQPFVTTKERGT
                     GLGLAISKAIITNHFGKISAANLPVRGAVFSIKLPVEREPNEAPRPIDVLLAVRDEMI
                     AYPTEQVLHAAGFTVNIPKSIDQLSDLADRCRPTILILDSAATAKQLLEIRHFWPEAK
                     ILIIGEPKSPGNSPEIRILPKPLNYGRLIELVRSMVGINPPSLTH"
     gene            complement(10075..11385)
                     /locus_tag="VF531_14400"
     CDS             complement(10075..11385)
                     /locus_tag="VF531_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7715203.1"
                     /translation="MMEFYQEPARAIPIRADVEVLVVGAGPAGFSAAVNAARNGARTM
                     LLEQSGSVGGVATSGQMSHWTGSTRGGFYEELLERANDFADGRVQFEHCPARQVINPE
                     KLKTVMLEMLHEAGVMLQLYTFVCEPILEGERITGVVTESKSGREAIKAGIVIDASGD
                     GDIAARAGVPFYKGRESDGKMQPMTLMFKVAGVDTERAVFPGGFEETYQVPAGDLQTL
                     GRRNLPFPAGHVLLYRTSLPGVVTCNMTNCIDVDGTSVTDLTRADYFCRLQLDRIVDF
                     LHTYVPGFEQCYLISSGAMIGVRETRHFQGEYTLTEQDILNARVFDDWAVTKAHFNFD
                     IHNLSGSGLDETGCQKKFGQPEGYTIPYRSLVPLKIDNLLLAGRNISGTHLAHSNFRV
                     MPICVNMGQAAGTAAALCIQDRLSPRRLDVGKLQERLRMHGVEP"
     gene            complement(11408..12274)
                     /locus_tag="VF531_14405"
     CDS             complement(11408..12274)
                     /locus_tag="VF531_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715204.1"
                     /translation="MQADGILAREGHAGFHISKLIWTILMFCAGMIMIIPFLWMFATS
                     FKIEGDVFKFPVEWFPKVWNFANYREVWLNQQPPFYVYYWNSIKIAVICVIGDLFTSS
                     MAAYAFARLEFKGKNSLFLLYLATMMIPFQVLMVPQFILFRQLGLYNTHWALILPQLF
                     TPLGTFLLRQYFLSIPQELSEAARVDGAGEFKIFWKVILPLAKPALASLAILAFVWRW
                     NDYDRPLIFLTNRNLYTITLGLTNFVDEAGIQENTLIMAASTSALLPMLIIFILGQRY
                     FVDGITAGSVKG"
     gene            complement(12297..13238)
                     /locus_tag="VF531_14410"
     CDS             complement(12297..13238)
                     /locus_tag="VF531_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715205.1"
                     /translation="MSFRNRTDPELQKMQRAKRIRALKKNLTGYLFIAPNFLGFLVFI
                     LIPVVFSLIVSFSDWNLFQGLGGIRFIGFRNFAAILKDSTFIDSVKNNLLFTIGTIPV
                     IMISALFIAVILNDKVYGKVAIRGMFFAPYISNIAAISVIWMLLYNPMHGPLNQFLRS
                     LGISNPPQWLASTKWALPALMILTIWMTIGYNCVLYMAGLQGIPKELYESSSIDGANA
                     FQKLWKITIPQLSPTTFFLLITNLISSFQVFGPVNIMTQGGPASSTSVISFYIYVSGM
                     RYFKMGYAAAMAWFLMLGIFVVTLIQWHGQKKWVNYL"
     gene            complement(13319..14638)
                     /locus_tag="VF531_14415"
     CDS             complement(13319..14638)
                     /locus_tag="VF531_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715206.1"
                     /translation="MMKRWRWLPALVVTVLFLTMISLTLAAPKAVKLVVWGGVPPENG
                     PQQIVTEWNAAHPETQVEYVRFVNDDAGNTKLDVALLSGEQIDAYFTYGVPYMVKRIE
                     GGMAEDLSKYGFTSFVKNNIGTSGIFRYKNKLYSIPTCKEMQYYMVNKNLFDQAGIAV
                     PTAWTIDDFRAVSKKLTRVADGKTQYGVFYLPFNMSNNFDLSRNVLGTNYSYKKGGKE
                     SNFDHPIFRQEAQLFYDMMYTDKSTFPYSEVLARKLQAYAQDLLMNQEVAMMKTAPWT
                     LRYVKDTGKYPHDWITSFTPLPTVPKVKNFYNDGALNNWLLMNSRSKYKKETAAFMKF
                     WVTNGWKAMLVGGKIPTYNKADQDQIIDGLFGDSKKLFDVAAFKRVAFDPKMKFYEPD
                     TITAAAPEILQIRQTEADKYFLRDETLDQFITNLKKRSDDAIRNAAQ"
     gene            complement(14810..16453)
                     /locus_tag="VF531_14420"
     CDS             complement(14810..16453)
                     /locus_tag="VF531_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715207.1"
                     /translation="MLKILLVEDELLVRMGLKTAIPWEQLGFQIVGEAENGLKALELA
                     REKLPDLVLTDIKMPKMDGIALIKALKKELPRIKVIVLSCYQDFDLVREAMQLGALDY
                     IPKLSMQPEDLIKVFTKVQCGLEEDQDGPGFTGAGDSYLNIHEMRSLFFNGLLKKDGP
                     SPEPITPPKVLRVSLPDHGEMGVICLGIDDAKSVRGDHTQDLSSSRDSLLSVVDEFIE
                     IKGWSGNIFNCEGRYGLIIGAGNKNPNISMRQLQEFCVQLMNEIQQYLNLSVSFGIGP
                     VIDDLSHFRTAFLQAAEALEFRLYNGKGSINIYQNIGETKDDVYSWPTEKALRELVED
                     GKITQAKTLIFEIITQIEANGKIAPSKVKRELIEILNTLSSLMKQYGGELEDLKDLGG
                     LDPYRSLEGTDTLIEIQDWFERFLNQYGGALSDLKVRRFGREISKSLEYINQHYADDL
                     TLTEIAGYIGLNETYFSHLFKKTTGVNFTDYVNSLRIDKAKEILRTEDYSIYQVAEKV
                     GYTNISYFSRIFKQWVGVGPFEYKKSSKNSDRNSKDSNN"
     gene            complement(16446..18230)
                     /locus_tag="VF531_14425"
     CDS             complement(16446..18230)
                     /locus_tag="VF531_14425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715208.1"
                     /translation="MTIRNKMIFSIICFVIVPMFFIPMLMFSNYKGIVEDKINRSTQQ
                     TLAQVANNMGTVFDNMISASNMLCFDSELIDSLRRYKGSSTWERYLFQTRIETKITNV
                     QNATLYPYNSDIVMIDFKGDIFSSSSYHSTVNYSRLSQEDWFKKTIAMNGYMLWMAPL
                     GDYLNPEGDDRKNIAMARLIKDPSNGNSYGIILISLYPEMRLESPYKAGAQWGGSQLL
                     LLNNQGKIILGNEPALVGTTYQHWRVLLPFLKQTSGDNSFVLKIAGQKTVGNYYLLSK
                     TDWKIVQLIPYKVLMRDIDRLRSYQITINLLFLAALILVTVFISWTITTPLHRLSLLM
                     RQVPKGDFSLRADIKGRDEVAELGNDFNIMVREMEQLIKQLQEAQALREKSRLEALQA
                     QINPHFLFNTLNDIKWLASINRAGNVSQMIAALGKLLETTLGKNEELITLTEEISCIK
                     SYILLAKMRYGDRFEVVYEIDPELLNCRIPYLLLQPLVENAIIHGFEDMESDGLIRIR
                     GYRQQENLLIEVADNGKGIDSDRLKILLTMPNPQSGRFSNIGIKNVSERIVLYYGREY
                     GLTVESKEGSGTSIRLLLPCRWGGEASA"
     gene            complement(18416..20206)
                     /locus_tag="VF531_14430"
     CDS             complement(18416..20206)
                     /locus_tag="VF531_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7715209.1"
                     /translation="MKDLVKKEYEVVVVGGGMAGVCAAIASARHGAKTALVHDRPVLG
                     GNASSEIRMHICGADCHASRPDARETGIIEEILLENRRRNPQHSFSIFDTVIWEKVRF
                     QEGLDLYLNAAMTGVTMGNGQIEKIMVHQLTTEIAYELTSNIFIDATGDGYLAHLAGA
                     DYKVGRESSAEFGESLAPEIGDRKTMGNTLMFRAVDTGKPVRFQKPVWAYSFTEEDLA
                     YRGHSASLAPMAHYGVDSGYWWIELGGEQDTIRDGEAIRDELLKTVYGVWDHIKNSGD
                     HGAENYALDWVGFLPGKRESRRIMGDYILKQQDLLEGRVFADAVAYGGWPMDVHPPEG
                     FKHRGEPTDFNHLKTVYTIPYRSYYSRSISNLMMAGRDISATHLAFSSIRVMATCAVG
                     GQAVGTAAAMTVQYHCLPVEIGKYLPELQQRLLKDDCYIPGLQNCDPADKALGGTVTV
                     SSFIEGCPGSSVINGVSRTVGDSANCWVSDGATAAGQWLELQFKQGQPIGEVYLKFDS
                     NLSQEIMTSLSKVTLSRQNPGLPSRLIKDYELQILDGTEIKFRLAVHDNYMRFRKHRI
                     NPQVSGDRIRVIVGSTYGDSAARIYEVRVY"
     gene            20368..>21152
                     /locus_tag="VF531_14435"
     CDS             20368..>21152
                     /locus_tag="VF531_14435"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HEX7715210.1"
                     /translation="MVAANHSALRKYLDFITRNYQINLCFNDFTGFVALDKDLDEILQ
                     PLMAHTNPYCMYIKSDRACFNRCLSLKKKIAEKSIKKRQTFFGVCHAGVGEYIIPIYC
                     QEELVGVINAGLFQVQPALASYFIRKTCRGTPLSQENAIRLYLGSTRKTYPDLDTVAA
                     ILELVAAYLGNAYLTIMATHSGLSLGKRYRSSEDSILAHILDYIKCNYTEALTVPEIA
                     RFCHCSESYVNHLFKKRVGINIKMYINKIRIEAAQEELTRSDR"
BASE COUNT         5304 a         5590 c         4893 g         5365 t
ORIGIN      
        1 aaccaccatg tctgtatgca ccccgctggg gaagttgacg cagagtttat ggtcgcgggc
       61 ctcgttgcgc agccgggtac gaatcttgag ataagggcag tccttctcca gcgtgagcca
      121 gtaggttatc ggaagttcta ccgtgcaacg gaagcgatcc ctactgtcca acgttgccgc
      181 agccggtatt tccagcttca tttcaatttt gtagactccg cgcagccggt tggattcaat
      241 catcgtaatc gcagccgcat tgccaaggct ggtgatgatc gggtttcgtt gcggctgatt
      301 gctgatatgc gcactgccgg tttccccgct gtcggtgaag tagtggagat tatccatgat
      361 tcggccgctg gctttatgaa gcagggagaa agtaccgttg gaatggatga tcactttcag
      421 atgttcgttc tccaaaactc cgttctcccg ggcgatcagc ttccggtcca gcccctcttg
      481 gggttggaag atataattcg gcttcctcag acgtaaggcg tatgtggaat aacctatggc
      541 cggaacggcg gcatggatca aaatccggcg gcgattcgcg gcaaacttaa ttgccttgca
      601 gtccagttcc cgctcgaccc cgatcttcac cggctccgaa ctcaattcca catattcaat
      661 gggccttccc tcagcatcca cgatatcgta gaatacgtcg ccggtattgg ccccgtcgac
      721 cccgatcgac ccggcgccgc tgtctttggc cttctcgggc aggtcaatca ctaccggaac
      781 caccccttgc cgcggcaccc ccagggtatt aaacagtgtg atggtatgat cattgcggtt
      841 aaaacacttg gctgtattaa tcctccggaa taatgccgtc acgctgcgtc cggccacttc
      901 ctcggccact gtgttggcaa tggagaagtt atagagcata tcttcatggg cgcgatccac
      961 tgcagcaccg caaatactat catgggcgtg atttttaagt aacgcttccc aggctcggtt
     1021 aagattaatt ttggggtact cttttccgta aaccgccgcc aaggtcgcca aaggttccgc
     1081 caaataaagc aggttggttt ccaccctgtc attgaggagt ttcaatttaa tccttgaaga
     1141 gtgggtggcc ccgagcaaag cattgaaact accgtgctcc acggtggtat atcgcagttc
     1201 ccctttgtga acgggtaaat ttacttcatc cgattggcgg atgatggtat tcatatattc
     1261 atccagtgtg gtttgaatga tctccacatc cggcgatatt tgattcaggg cttgaatcat
     1321 ctccggcaat agagggaatg gctgatcatt atcctccata tttagggcca aaagttgatt
     1381 gccgatggca taaggcatgg cttgattcag gatcccccgc atcgcctgct ccagggagtc
     1441 gggatcgcgc cggaagttgg aatcctctct caataaagta aaggcttttt catatagtcc
     1501 ctcatcacaa aggtgtaccg gatcatgatt ccggtcatat gtataggtca ggtcccggca
     1561 aggcttcccc agcaccagcg gttggtggat gtaaaagtac cagttcgtcc gggtgacttc
     1621 atcaaaggtt ctgagcgcat agagtttgga accgtcggct ccctcccaac gatataacgg
     1681 cgccaggata tgcttgttgg tgccccggta gaagatggcg gtatcaattc cgaacccctg
     1741 gtaaatctgg ggcatttgtg aattttgacc atatgatgtg gcggtatacc ccgatcgcat
     1801 actgccgccg aattcccaag ccattttccg tcccaacagc aaattgcgga ttaacgcctc
     1861 gggagccacc gtattggtct cgggcaaggt ataccagttg acgatctgca ttttgccggt
     1921 ttcaatcaac ttgcggatcc ggtctttgtt ttccggccga atcgtcagat aatcctctag
     1981 aacaactgta ccgccatcca cacagaaatg ccggtattcc tggttttgct ccaaaagttc
     2041 gatgatggaa tccatcagat caaccaagcg taacttcgat tgttcggcgg tatgccgcca
     2101 ttcccggtcc caatgggtcc cgcagatgac atgcattaca tattttttag ccattttcgt
     2161 tgcctccgtc ttctacgttg cgtgaaatct gttccatgat atcgtctata tgattacatc
     2221 cggtgtatcc ggccttttta tcaatttcag attttgagtt aagcggccta aaaaatatac
     2281 aacagacttc gattcagtat cgataaggaa tgtgggacga gcctggttac gaacccgtcc
     2341 cacgttgaat taagctgttc tcactaaata attatttcat taacgccatg gcttcttttt
     2401 gagcctgttt caaaatatcc tttaccggct gattggtgat gacgattttg ctgaccgcgc
     2461 ggttcaagac atcccatacc ttgacatcgt tcttggcgaa agtgggcagg ccttgcacat
     2521 tacccttttc cgcatcgaag atcgccttca tgaacgggta tttggccaga taggcggggt
     2581 cttcggaaac cgaggccaaa accgacggcc gttgaccggt gctggcttgc agggtctgga
     2641 agcctttgtc catggtaata aattcgatga gtttaaaggc ttcatctttg tatttggcgt
     2701 atttaggaac tgctgcaaat gaggctccga tagtcacgtt ggccgcttta ccgcccttat
     2761 cgggaagcgg gatggtggat acgccgaaat cgagaccggc gcgcgctccc tcagccacac
     2821 gccacggacc gtcgatcaac atggcaaacc gttgttggtt catggcatcc caaaatgcac
     2881 cttcgctagg tgcggcggca atccccggag gcagatgctt ggagagcttg cgtaagtatt
     2941 caaatgccgt tacattggcg gctttatcaa gagtcggata tcctttgctg tcaacataac
     3001 cgccgccgag catcaacaca aacggggtat accgcaggga accgccgtag ttcggcccca
     3061 tataaacacc gccgccgtaa aactggccgt tacctgcttt atcgatcttt tcggcatatt
     3121 ccaaaatctg attccaggtc ttcggcggtt tctccggatc caggccggct ttcttaaata
     3181 gatccttgtt ccaatagaaa gcgttgaccc ccgggagccc cgccacaccg taaatcttgc
     3241 ctttatactc catcggcttc caagcggccg gaacgacgtt cttacgaatc ttggccgcta
     3301 aagcagcagg gaatggctcg taatacccct tcatggcaaa gccgggttgt tgggtttcac
     3361 cgacaaagat attgacctcg ctatccttag ccacaaacgc ggcgacctgc ttttgaacca
     3421 acggttcggc ccaaccccag tcatcggtga caaaggtgta cccgtcttta ttgaggaatt
     3481 tctcaatagc gatctcggtt tctaacggca tccggccgat agcgtccttc ttatccggat
     3541 ccagcttatc ctgtctcgct tgttccttgg gaatctcctc gatggcctta ccgacgattc
     3601 cacccaagtt taaactgtgg aactctttgt agtcggcacc ggaaactgcc gggatcatca
     3661 tcgccaacaa cagggcgacc gccagcaaca aacctaaata cttcttcaat tcactcactc
     3721 ctttgggttt gatttttata gccaaagaac ttccccgctc cattcctgcc gcgaaccacc
     3781 cctttcaaag ttggctgttc tactggaaaa tcaactgatt cctcaattcg cccggaatgt
     3841 tttatccctt gataccggta ttggccagac cttccaccat gtatttctgg aataaggcca
     3901 agattatcaa ggaaggaatc gttaatgcca tacctaaagt gataatcatc ggataattgg
     3961 gggtatcctg tttcacagtg aacatggcaa tgatacgggt aataccgccg ggcaaggtca
     4021 gtaaacgctc atcattggta aaaaagaagg gaaccatcca gtcattccag ttaccaatgg
     4081 ccgtggtgat ggcgatgtag gccaataccg gtttttgaag cggagccagc agcgaaaaga
     4141 tgatcctgaa ggtgctggca ccgtcaaccc gcgcagcctc atccaactcc atcggcgtac
     4201 ggagtaacga ctgacggacc agaaacgtac ccatgacatt cagagcacaa aaggtaaaca
     4261 agtatactcc gaaagtgttg tatagaccgt gaccgcccat tccgaaaatg ttattgcctc
     4321 ccgccagcgg ccaccggtgg agctcaacaa attgcgggat aaacgagact acacccggta
     4381 ccatctgggt tgtcaagagc agcaaaaata ccttatcccg ccccttccac ttgagccggc
     4441 tgaaggcata tcccccgagt aatgaggtca atacagccaa gaccgtcata tagaccgttc
     4501 ggagcaccga gttggtcaga atctgcggcc cataattaag gactgagccg acactggctg
     4561 tatcgatccg gattcggccc ttggttgctt cttcggaagg taaatcaaaa atggtcttat
     4621 ccgacgtgaa aaacatcgta taatttctca gcgtaggatg ctgcggccag gggaaaagcg
     4681 tatcgctttt tctggttgca aattcgtaag tagtaaagaa tccgaccaaa aaggagaaga
     4741 tcaatgggta gattaatacg gcgccaccca aggccaagat cagatgagtc agccagttcc
     4801 cggcccgttt ttttctttga ggggctgttt gcaactgagt cgcgttactg ttcattgcca
     4861 ttgtcactcc tcctccttat ctcgggtaac ccagttcacc aagaccaacc cgaaaactac
     4921 agccgccaaa atcatggaac cggcgcctgc catgcctaga ttgaaatcga cgaacgcatc
     4981 cttatagatc tgggacatca agacgttggt gctgccaaac ggtccgccgc cgctgatgaa
     5041 caatacctgt tcgtagattt gaatcgccgc gatgcaagcg ttgataataa tgaaagtgat
     5101 gaaaggccgc aaaccgggaa tcgttacgtg gcggaagcat tgccagttac cggcgccgtc
     5161 gatttttgcc gcctcaaata actgcggatt caccgattgt aacccggcca accacaggat
     5221 ggcggaaacc cccaaccctt tccaaactgt atagaaaacg atccaaaaga ccatccatcc
     5281 ggtgctgtat tgccagatga ccggttgcgc gccgaacttt tggataatgt tattgaccaa
     5341 accgatctgg gacggctcaa gccacatgtt gaaaatctgg gtggtcgccg ctgccgaggt
     5401 gaccgcggga acgtaccaaa tagtccggta aaatccccgg ccccggatgg gcatgttcat
     5461 caacaatgcc gcccccagcc cgagcaggtt ggtaacagcc gcggttaaaa ggccaatcca
     5521 gatggaattc catagtgtca ggcgataatt gggatcaccg aaaaaagtga aaaaattttg
     5581 aatcccgttc cattttggtg tctcaagtac gttatgccaa tcgaagaaac cgagcaataa
     5641 tccgaaaaac attggcccgc cgctaaccac gaaccaccac cctaacagcg gaaccaagat
     5701 aattgccgct ctaaccccat cggggtgttt acgataaaac cgtgcgaagg ggtttattct
     5761 gggttcggta agtcgttgga tacccgcatt tgttgtattc atctatgtac ctcctcaatc
     5821 gattcatctt catcggcttg cttcccaacc aggcgtctat gtctttggat ccggcgggca
     5881 agttgaggcc cgaacgacca tttccgctgg aagaataacc ttttccgtat ccgccgcggg
     5941 attccgaatg cttttcagta acagttgtgc ggcgttctgc ccgatctcct ttttgggttg
     6001 gttgaccgta gttaacggca cctcaacatt ggctgataaa agattattgt caaatccgat
     6061 gatattcaaa tcctcaggca ccctgatccc aagctgcaga gcggcccgca ggacgcatat
     6121 ggccgtgtaa tcgttgattg caaaaattgc agttaaatcg gggtttttct ttaaataacc
     6181 ctgaatggtc ctgaccgcct cggggtccgc ccgcccgatt tgatgattgt aaacctgctc
     6241 ccgttccaag gtcagttcca tccgctggcc gggcaaggta attcccatat ccgccagagc
     6301 ctgggtaaac ccacgaatcc ggctgttgat gctaattaca ttctccggcg aatatcccac
     6361 caaaccgata cgacggtgac ccaaatcaat cagatatttt cctgccgcat aggctccgcc
     6421 aaaattgtcc gagaccacat aattggtatc gataccccgg aaaaagcggt caaccagtac
     6481 aaaggggaac ccatcgacct taagtttgat taaggcatta ctgcagtacg caccctcgat
     6541 ggggaagatg atcaagccct gaattccttc ggcccgaagc gtttcgatgg cccggttttc
     6601 ttcagccatg gaattgtcgg atttcctgaa gactaaatga taattcaggt tgcgtaattc
     6661 cctttccgcg ccgctgacaa tgcccgcttc ataggaatcg ttgagatagg gcaggatcaa
     6721 gcccacattg gccgtgccgg tcggatatat ctttccgttc tcgctggtta ccgcctcgaa
     6781 ttgttcataa ggacggctgt tgttgttcgc caaacccgcc tccggctgcg aaggtaagct
     6841 cttcacaaaa gttcccttgc cgggcacacg ataaacaatt ccttcctgca tcagttcgag
     6901 caaggcttgt ttggtagtga tccggctgac cccgtatttt tgggccaatt ccacctcaga
     6961 agcaatccgg gacccggctt taaattcacc tgaggtaatc gaacgcatga ttaattcctt
     7021 gatctggcta tacaacggca tggcgatctc tttattcacc gtttcggctg ttttctgaga
     7081 gagttgggtc cgtttctgtt ccattgggtt aatgactcct gagaaatatt ggttcttatg
     7141 tttataatcg attattaccg gatatatcag gttatataaa tgattccatg acgttacaaa
     7201 aactccttct tatttttaaa attttttaac tttggttgcc acagctggat gtccatacga
     7261 ttatccctgc atataccggc gtggtccaaa aaatacccgc aacacatgat acagttgcag
     7321 gtaaccatca aatgtcggcg cttatgtaag attaaaatcc agtacctgga tattttgtat
     7381 attacgaaaa aaacgggcgg caggaaaagg ttggcgattg tcgaaatatc aaaaagtttt
     7441 gatctcttag gggatcagga gtggttgaat gaattccatg agattggaaa cctgtacatt
     7501 aatgtgtaat gagatgttaa ctacagtggg acaggctatc ggaatccatg tattgctgct
     7561 cgttatcgag cattcgttat ggcttgccgg tcagcaatat aaggaagcag ccttaatccg
     7621 tttctcggaa cagggagtct ttcttgatcg tttaaatgat atcgaaccgg aaaaggccat
     7681 agcggtcgca tacgagttca ttacggcaat tatggccacc ctcgggaagc ttatgggaaa
     7741 acaacttgcg aagcagttca cggaggaatt acagattgaa cggattcaat tgaaataaac
     7801 gattgataac gatttcgtat aatcggtgcc aataaagcat ggggtcggga ttatgaagtt
     7861 aaatcattta gtcaactccc gccgctgggt ggattttttg gaaagtaccg ccatgggact
     7921 tcaactcaat ctggcattaa ggtcgctgac cgaaccggcc attggattgc aagcacccgg
     7981 ggtttgcccg gattgtaacc gggtttatcc ccgcctcacc gtggaggatt tggcggcggt
     8041 aaaaaccttc catccccgtt cgcctcatca aaacgaggaa ggacatgagt ttgtaacccg
     8101 aaactctgaa ccgttttttt cccttcctgt ccaggatgga ttatttctca tcgccagaga
     8161 atgtccctgt aaaaccgcaa caggtcttct ctccctgtcc caacgggcca aggtcgccca
     8221 gaaactgttg agcgcctttt tagacacctt atcggaagga attgccggga gccacgcggc
     8281 acttgaattt tcagctctgc ggcaaatcaa ccagatcctg ctttcattgt ttcgcggtga
     8341 atccggtgcg tcccggaggg cttttgatct gatattaagc gccctgatca ttatgttgga
     8401 cgctgatgcc agttggcttg agtatcgcga tgaaaccggg gaacagcgga tcttcaaggg
     8461 agaaccggcg gcaatcgcat ctcatcttcg gatgaaggtt cccggggaaa ttctgcagat
     8521 tgaggtcgag ggagcgtaca ctaagggttc tctcagtttg ttacggcccg gggatccaaa
     8581 gcttgccgcc agtttactcc ccttaatggc ccaagaatgc agtattgttt ttgaggttga
     8641 acatctgttc cgcctgatgc agtcccagct ctcccggttg ctgggcgcac tcaactccgc
     8701 tgtgttcttg gtcaatgaac acatgcaaat cgcctacctt aatccggcag ccgaggcgct
     8761 ccttcaccaa tcagcctttt ccttgattgg aatttcaatc ggagttttac cgcaattttg
     8821 ggcggatcgg atctcaaacc aggttgatga gccgatccgt gattacaaga cgacatttca
     8881 ccggaacaca gattccatcc tgcttgactg gcagatcctg cccctacgtg acgacacctt
     8941 ggttgcaggc tggttaatca tagccgatga tcgtactgac gcctatcact ggcaggaaat
     9001 cggccgccag gcggagagtt ttacgacagt agcctcggtg atcagtcccc tcgcccatga
     9061 attgcgtaat cctttggccg cggccaaagg attgctgcaa ttggttcagc gtcacaatga
     9121 tccggataag gtttccggat atatcaacct gatcttgcgt gagattgatc gaatgagtat
     9181 attggtaaat gagttcttgc agattggccg gagtacgggc ggtcactttg agtccgtcac
     9241 tctcagctcc ctggtcgagg atctgctccc gttagtcgaa ggcgaatcgg ccgccggagc
     9301 agtcgagatc cgttgcaatc tggaacccgt cccttcagtg agagcggacc cgggacaact
     9361 gattcaagtc ctgctcaatc tgatccataa tgctgttgag gcggccggac cggagggccg
     9421 ggtcgaaatc gacctgaagt ctcaaggaga ctggatcgtt ttagaggtca gcgacaatgg
     9481 cccgggaatc gaagcggagg tcttcgggaa actctttcaa ccattcgtca ctaccaaaga
     9541 acggggaacc ggtctagggc tggctatctc caaggccatc atcaccaacc acttcgggaa
     9601 gatcagcgcc gccaacctac cggtaagagg ggcggtattc tcgatcaaac tgcccgtgga
     9661 acgggagccg aatgaagctc cccgtcccat tgatgttctg cttgccgtcc gcgacgaaat
     9721 gattgcctat ccgacggaac aggttttgca tgccgcgggc tttaccgtca acatcccgaa
     9781 gagcattgac caactttcgg atttggcaga ccgttgccgc ccgaccatct taatcctgga
     9841 ttcggccgca acagcgaaac aactgctgga gatccggcat ttctggccgg aggccaagat
     9901 tctaatcatc ggggaaccta aatcccccgg gaattcaccg gagatccgga ttctgccgaa
     9961 accactgaat tacggcagat tgatcgaact tgtccggtcg atggtgggga taaaccctcc
    10021 gtcactaacc cattgaacga ctatattcat gggtaaatct ttggaaatta ccatctatgg
    10081 ttccacccca tgcattcgta atctttcctg caatttgccg acatccagtc tccggggaga
    10141 taatctgtct tgaatacaca aggcggcagc ggttcccgcc gcctgtccca tattcacgca
    10201 gatgggcatc acccgaaaat ttgagtgggc cagatgggtt ccggagatat ttctccccgc
    10261 caaaagcagg ttgtcgatct tgagcgggac caacgagcgg tatggaatcg tatacccctc
    10321 gggttggccg aatttcttct ggcaacccgt ctcatccaaa ccgctaccgc taagattatg
    10381 gatatcaaaa ttgaaatgtg ccttggtcac cgcccaatca tcaaagaccc gggcattcag
    10441 gatatcctgc tcggtcaggg tgtactcccc ttgaaaatgc cgggtctccc ggaccccgat
    10501 catcgccccg gaactaatca gataacattg ctcaaacccc gggacatagg tatgtaaaaa
    10561 atctacgatc cggtccagtt gcaggcggca gaaataatcg gcccgggtca gatcggtaac
    10621 ggaagtcccg tcaacatcga tacagttggt catattgcag gtgactactc ccggcagact
    10681 cgtccggtaa agcagtacat gtccggcggg gaaaggaaga ttcctgcggc ccaaagtctg
    10741 aagatcccct gcgggtacct gataagtttc ctcaaaaccg cctggaaaga cggcccgttc
    10801 ggtgtctaca ccagccactt taaacatgag cgtcatcggc tgcattttac cgtcgctctc
    10861 ccggccttta taaaaaggaa ccccggcccg ggcagcgata tctccatcgc ccgaagcatc
    10921 gatgactatg ccggctttga tcgcttcccg gcccgattta ctctcggtaa ccacgccggt
    10981 aatacgctcc ccctcaagga ttggttcaca cacgaaggta tataactgta acataacccc
    11041 tgcctcatgg agcatctcca gcatcacggt cttcaatttt tccggattaa taacctgtct
    11101 tgccgggcaa tgttcaaatt gcacccgacc atccgcaaaa tcgttggccc gctcgagaag
    11161 ttcttcgtaa aaaccccccc gggtgctgcc ggtccagtga ctcatctgtc ccgaggtcgc
    11221 tacccctccg actgagccgg attgttccaa aagcatggtt cgggcgccat tccgtgcggc
    11281 attcaccgcg gctgagaaac ccgccggtcc ggctccgacc actaaaactt ccacatccgc
    11341 ccggatgggt attgccctgg ccggttcctg ataaaattcc atcattctca cctctccaaa
    11401 ttccgtttta tcccttgaca ctgccggcgg tgattccatc cacaaaatat ctctgaccca
    11461 gaataaatat gattaacatg gggagcaagg cactggtgga agccgccatg atcagcgtgt
    11521 tttcctgaat gcccgcttca tccacaaaat tggttaatcc caaggtgatc gtatagagat
    11581 tacgattggt caaaaaaatc aacggccggt cgtaatcgtt ccaccgccag acgaacgcta
    11641 aaatggccag actcgccaag gccggtttgg cgagtggcaa aatcaccttc caaaatattt
    11701 taaattcgcc cgcgccgtca accctggcgg cttcggacaa ctcctgagga atcgatagga
    11761 aatactgccg caataaaaag gtccccaggg gcgtaaataa ttgcggcaaa atcaaggccc
    11821 agtgagtatt gtacaatccc agctgacgaa agagaataaa ctgaggaacc atcaatactt
    11881 gaaacggaat catcatcgtg gccaaataaa gcaggaacag gctattcttg cctttaaact
    11941 ccagcctggc aaaggcatac gccgccatgg aactggtgaa cagatcgcca atcacgcaga
    12001 ttaccgcaat tttaatggaa ttccaataat agacgtaaaa aggcggttgc tggttcagcc
    12061 atacctcccg gtaatttgca aaattccata ccttaggaaa ccattccacc ggaaatttaa
    12121 atacatcccc ttcaattttg aaggatgtcg caaacatcca taagaaagga atgatcatga
    12181 tcatcccggc acaaaacatc agaatggtcc agattaattt cgatatatga aatccggcat
    12241 gtccttcccg ggccagtatt ccatctgctt gcatcttcat caccccgccg taatcattat
    12301 aaataattca cccatttttt ctggccgtgc cattgaatca gggtcacgac gaatatgccc
    12361 agcatcagaa accaggccat ggctgccgcg tatcccatct taaaataacg catgccgcta
    12421 acataaatgt aaaaactgat cacactggtt gaactggccg gtcctccctg ggtcatgata
    12481 ttgaccggtc cgaatacctg aaacgaagag atcagattgg tgatcagcag gaagaatgtt
    12541 gtcggcgaca gttgcggaat ggttattttc caaagtttct gaaaggcatt ggcaccgtcg
    12601 atcgacgacg attcatagag ctctttgggg attccctgca gccccgccat atatagcacg
    12661 caattatatc cgatcgtcat ccaaatggtt aagatcatca gggctggcaa agcccatttt
    12721 gtactggcaa gccattgcgg cggattgctg attccgagac ttcgtaaaaa ctgattgagg
    12781 ggcccgtgca tcggattata gagcaacatc caaataaccg agatcgcggc gatgtttgaa
    12841 atgtaaggcg caaaaaacat gccccgaatg gctaccttac cgtagacctt atcgtttaaa
    12901 atcacggcga tgaagagcgc cgagatcata atcaccggga tggttccaat cgtaaataac
    12961 agattgttct tcaccgaatc gatgaaggta ctatccttca atatagccgc aaagttcctg
    13021 aatccgataa accgaatccc acccaaaccc tgaaagaggt tccagtcgga gaaggagacg
    13081 attaaggaaa aaacgaccgg gattaagata aagactaaaa agcccaaaaa attgggggcg
    13141 ataaataggt agcccgtcaa attctttttg agtgcccgta tccttttggc cctttgcatc
    13201 ttctgaagct cagggtctgt tcgattccgg aaggacatgc gctctcactt ccttttttta
    13261 ttaagaagga taggtttttc aacctatcct tcagtccgtc actggttaaa tactgaactc
    13321 actgagcggc attcctgatg gcatcatccg aacgtttctt taaattggtg ataaattgat
    13381 ccaatgtttc atcgcggagg aaatacttat ccgcctctgt ctggcgaatt tgcaggattt
    13441 ccggcgccgc ggcggtgatg gtgtcgggtt cgtaaaattt cattttggga tcaaaggcca
    13501 cccgtttaaa ggccgctacg tcgaagagct tcttgctgtc cccgaaaagc ccgtcgataa
    13561 tctgatcctg atccgcctta ttgtaggtgg ggattttgcc tcctaccagc atggccttcc
    13621 aaccgttggt aacccaaaac ttcatgaaag ccgccgtttc cttcttgtac ttactccgac
    13681 tgttcatgag cagccagttg ttcagagcgc cgtcattata gaaattcttc actttgggaa
    13741 cggttggcag cggcgtaaag ctggtaatcc aatcatgggg atatttgccg gtatccttca
    13801 cataccgcag ggtccaaggg gctgttttca tcatggcaac ttcctgattc atcaataaat
    13861 cctgggcgta tgcctgaagc tttctggcaa gaacctcgga ataggggaag gtgcttttat
    13921 ccgtatacat catgtcataa aacaattgtg cttcctggcg gaagatcggg tgatcgaaat
    13981 tggattcctt gccgcccttt ttatacgagt aattcgttcc cagaacattc cgggacaggt
    14041 cgaagttatt gctcatgttg aatggcaggt aaaaaactcc gtattgcgtc ttgccatcgg
    14101 caacccgggt caattttttg ctgactgccc ggaaatcatc gatggtccat gcggttggta
    14161 cagcgatccc ggcctgatcg aacagattct tattgaccat gtaatactgc atctccttgc
    14221 aagtggggat gctgtataat ttgtttttgt accggaaaat tccgctggtg ccgatgttat
    14281 tcttcacaaa tgaggtaaag ccgtatttgg ataagtcctc ggccattcca ccctcgatcc
    14341 gtttcaccat atatggaacg ccgtatgtga aataggcatc aatctgttcg ccggataaca
    14401 acgccacgtc tagcttggta tttccggcat catcattgac aaaccggacg tattcgacct
    14461 gcgtttcggg atgggccgca ttccattcgg tcactatctg ctgcggtccg ttttccggcg
    14521 gcacgcctcc ccagactacc aatttaacgg ctttcggggc ggccagagtc aaagaaatca
    14581 tagtcaaaaa gagtaccgtt acgacaagcg ctggcaacca tctccacctt ttcatcatga
    14641 accctcctcc aaagtttttg gcgatgtacg tgcaatgatg cgcacgatct atccttgccc
    14701 cttcattgta actcgaagcg aatttacgac atattaacct gtttgtcttc aagttgaaat
    14761 atttttggaa ctttttttcg cggggatccg gtttttcctt gaaagggggt caattgttac
    14821 tatctttaga atttctgtca ctatttttgg atgacttttt atattcaaac gggcctaccc
    14881 ctacccattg tttgaatatc ctgctgaaat aactgatatt ggtatagcct accttctcgg
    14941 ccacctgata gatgctatag tcctcggttc taagaatttc tttggcctta tcgatccgta
    15001 acgagttgac gtaatcggta aaattaactc ccgtggtctt cttaaacaaa tgactgaaat
    15061 aggtttcgtt taaaccgata tacccggcaa tctctgtcag tgttagatcg tccgcataat
    15121 gctggttgat gtactcaagg gacttactga tctcccgccc gaaccgtcgt accttcaaat
    15181 cggacagagc gccgccatat tgattcaaaa accgctcaaa ccaatcctgg atctctatga
    15241 gtgtatcggt accctccaaa gaacggtacg gatccaggcc gcccaaatcc ttcaaatctt
    15301 cgagctcccc gccgtactgt ttcatcaagc tcgacaaggt attcaaaatc tcgattaatt
    15361 cccgttttac cttggacgga gcgattttac cattcgcctc aatctgagtg atgatctcaa
    15421 aaatcaaggt ctttgcctga gtgatcttcc cgtcttccac caattcgcgg agagctttct
    15481 ccgtaggcca gctgtagaca tcatccttgg tttccccgat gttttgataa atattgatac
    15541 tgcccttgcc gttataaagc cgaaattcaa gcgcctccgc agcctgaaga aaagccgtcc
    15601 ggaaatggga aagatcatca atgacgggac ctatcccaaa ggaaaccgat aaattcaaat
    15661 attgttggat ctcattcatc agttggacac aaaattcttg aagctgcctc atggaaatat
    15721 tcggattctt atttcctgca ccaatgatca agccatagcg gccttcgcaa ttaaaaatat
    15781 taccactcca tcccttgatc tcaataaatt catcgacaac gcttaacaat gaatcccggc
    15841 tggaactcaa atcctgcgta tggtcgcccc gaaccgactt ggcatcatcg attcccaggc
    15901 agatgacgcc catctcccca tgatcgggga gggaaacccg caggacctta gggggggtta
    15961 tcggttccgg ggaaggcccg tccttcttaa gcaaaccatt aaaaaagaga ctccgcatct
    16021 catggatatt gaggtaagag tcccccgccc ccgtaaatcc cggaccatcc tgatcttctt
    16081 ccagaccgca ttgtaccttg gtaaatacct tgatcagatc ttccggctgc atcgatagtt
    16141 tggggatgta gtccaaagcc cccaattgca tcgcttccct gaccaaatcg aaatcctggt
    16201 agcaacttaa tacgatcact ttgatccggg gcagctcctt tttgagggct ttaatcaagg
    16261 cgatcccgtc catcttgggc attttaatat ccgtcagaac caggtccgga agtttctcac
    16321 gggcgagttc caaagccttt agaccgtttt ccgcctcccc cacgatttga aaacccagct
    16381 gttcccaggg gatggcggtt ttcaaaccca ttcggaccag taattcatct tcaaccagta
    16441 atattttaag cactcgcttc tcccccccat ctgcaaggca agagcaaccg gattgaggta
    16501 ccggatccct ctttgctttc cacggtcaac ccgtattctc taccgtaata caaaacgata
    16561 cgctcactga cattcttgat cccgatatta ctgaaccgcc cgctttgggg attgggcata
    16621 gtcaacagga ttttgagccg gtcactgtca atccccttgc cgttatccgc cacctcgatg
    16681 agcagatttt cctgttgccg gtagccgcgg atccgaatca acccgtccga ctccatatcc
    16741 tcaaagccat gaataatcgc attctccacc aacggttgca acaagagata aggaatccgg
    16801 caattcagca attccggatc gatctcgtaa accacctcaa agcggtcgcc gtacctcatt
    16861 ttggccaaca ggatataact cttgatacaa ctgatctcct cggtcaaggt gatgagctcc
    16921 tcatttttgc ccaaggtagt ctcgagcagc ttccctaaag ccgcaatcat ctgactgacg
    16981 ttgcccgccc tgttgatcga agccaaccat ttaatgtcat tcaatgtgtt aaagagaaaa
    17041 tgaggattaa tctgagcctg taaggcctcc agacgcgatt tttcccgtaa agcctgagct
    17101 tcctgaagtt gtttaatcag ttgctccatc tcccggacca tgatgttaaa gtcatttccc
    17161 agttcggcca cttcatcacg gcctttgata tccgcccgca gcgaaaagtc gcccttggga
    17221 acctgacgca tcagcagact taaacggtgt aaaggagtgg tgatggtcca ggagatgaag
    17281 acggtaacca ggatcaatgc cgccagaaac aatagattga tggttatctg gtaagaccgt
    17341 aatcgatcga tatctctcat caacacttta taggggatca attgcacgat cttccaatcg
    17401 gtcttggaca ggagataata attacccacg gttttctggc cggcgatctt caaaacaaat
    17461 gaattatccc cggaagtttg tttcaaaaac ggcaataaaa cccgccagtg ttgatacgtt
    17521 gttcccacca aagccggttc gttaccgaga atgatcttac cctggttgtt gagtaaaaga
    17581 agttgacttc ctccccattg ggccccggcc ttatagggcg attccaatct catctccgga
    17641 taaaggctga ttaagatgat cccgtaactg ttaccgttcg aaggatcctt aatcagccgg
    17701 gccatcgcga tatttttgcg atcatccccc tcggggttca gataatcacc gagcggagcc
    17761 atccacaaca tataaccgtt catcgcaatc gtttttttaa accagtcctc ttgagatagc
    17821 cgggaatagt ttacagtgga gtgatacgac gacgaggaaa agatatcccc tttaaaatca
    17881 atcatcacaa tatccgagtt ataaggatac aaggtagcgt tttgtacatt cgtaattttt
    17941 gtttcgatac gggtttgaaa gagatacctc tcccacgtgg atgaaccttt atatcggcgc
    18001 agactgtcaa tgagttccga atcgaaacaa agcatattgg aagcggaaat catgttatca
    18061 aagaccgtac ccatattatt agcaacttgc gccaaggttt gctgcgttga ccggttaatc
    18121 ttatcctcca cgatcccttt gtaattggag aacatcaaca tcggaatgaa aaacatcggt
    18181 acaatcacaa aacagataat ggaaaagatc atcttattgc gaattgtcat aaaccgcacc
    18241 ttctgttgga tctcgattta tatacaatac ttcagccagg atcccgttta tcctgccggg
    18301 aaggaacgat ccgttgattc ccatccgcaa aagtgatccg ctgcttgatt ttatccccgg
    18361 gtgcacggaa cacgaactgt tcttttgaga cagcccccgc caccagggtg agagcctaat
    18421 aaacccggac ttcataaatt cgggccgcgg aatccccgta ggtggacccg acaatgactc
    18481 tgatccgatc gccgcttacc tgtggattga tccggtgctt ccggaatcgc atataattat
    18541 catgaaccgc caaacgaaac ttaatctccg tcccatcgag gatctgcagt tcataatcct
    18601 tgattagccg ggaaggaagt ccgggatttt gacgcgataa ggtaaccttg gagagggaag
    18661 tcatgatctc ctgagagagg ttggaatcga atttaaggta gacctcccca atcggctggc
    18721 cttgtttgaa ttgaagctcg agccactgcc ccgccgccgt cgcgccgtcc gaaacccaac
    18781 aattggccga atcgcccacc gtacggctga ccccgttaat cacactggat ccggggcaac
    18841 cttcgataaa tgatgaaaca gtgacagtcc cacctaacgc cttgtccgcc ggatcacagt
    18901 tttgcaaacc gggaatgtaa caatcgtcct ttaatagtct ctgttgaagt tcaggcaggt
    18961 atttgccgat ctccaccggc aggcagtgat attgtaccgt cattgccgcg gctgtcccca
    19021 ctgcttgacc gcctacggcg caagtcgcca ttacccggat ggagctaaag gccaaatgcg
    19081 ttgcgctgat gtcccgcccg gccatcatga gattggaaat acttctggaa taataggaac
    19141 ggtaggggat agtgtacact gtcttcaaat gattaaaatc cgtgggctcg ccgcggtgct
    19201 tgaatccttc gggggggtga acatccatcg gccatcctcc gtatgccacc gcatcggcaa
    19261 atacccggcc ttcgagcaaa tcttgttgtt tcaagatgta atcacccata atcctccggc
    19321 tctcccgctt ccccggcaaa aaacccaccc aatccagggc ataattttca gcgccgtgat
    19381 cgccgctgtt tttaatgtga tcccaaactc cgtatacggt cttcaaaagt tcatcccgga
    19441 tcgcttcgcc gtcccggatc gtatcctgct cccctcccaa ttcaatccac cagtatccgg
    19501 aatccacccc atagtgggcc atgggagcca gcgaagcgct gtgtccccgg taggccagat
    19561 cctcctccgt aaatgaatag gcccaaaccg gtttttgaaa ccggaccggt tttccggtat
    19621 caacagcgcg aaacattagg gtattcccca tcgtcttgcg gtccccgatt tccggggcta
    19681 agctttcgcc gaattcagca ctgctctctc tgccgacttt gtaatccgct cccgccagat
    19741 gtgcgagata cccatcaccg gtggcatcga taaagatatt gctggtcaac tcataagcaa
    19801 tctccgtggt aagttgatgg accatgatct tctcgatctg cccgtttccc atggtcaccc
    19861 cggtcatggc tgcatttaag tacaggtcaa gtccttcctg aaatcgtact ttctcccaga
    19921 tcacggtatc gaaaatcgag aaggaatgct gcggattccg gcgccggttc tccaggagaa
    19981 tctcctcaat gatcccggtt tcccgggcat ccgggcgact ggcgtgacaa tcggcaccgc
    20041 agatatgcat gcgaatctcc gagcttgcat tgcctcccaa taccggacga tcatgaacca
    20101 gggctgtttt cgccccgtgg cgggcgctgg cgatcgctgc gcaaactccg gccataccgc
    20161 cgccgaccac caccacttca tactcttttt tcaccaaatc cttcatggct tcgtcatccc
    20221 ttctataaaa ttctgttttc attatatatt gggccgaacc gggtttcctt gaattggctg
    20281 cggactatct gcaattttct gcacaattgc aaaacacctg tgcaacgaat ataatggtgg
    20341 tattgaagca aacggaggtt atcaccggtg gtggccgcca accattctgc attacgcaag
    20401 tatctggatt tcattacaag aaattatcaa atcaatttat gtttcaatga ctttacagga
    20461 tttgtggcgc tcgataagga tcttgacgag attttacagc ctttaatggc ccataccaat
    20521 ccctattgca tgtatattaa atccgatcgc gcctgtttca accgttgcct gtcattgaaa
    20581 aagaagattg cagaaaaatc cataaaaaaa cggcaaacct tctttggggt ctgccatgcg
    20641 ggggtgggag aatacatcat ccccatttat tgtcaggaag aattagtggg cgtcatcaat
    20701 gccggtttgt tccaggtcca accggcattg gcatcctact ttatccgcaa aacctgccgg
    20761 ggaacacccc tcagccaaga aaacgccatc cgactatacc tggggtcaac ccgtaaaacc
    20821 tatcccgatc tggatacggt cgcagccatc ctggaattgg tcgccgctta tctgggcaat
    20881 gcctacctca ccatcatggc cactcattcc gggctcagtt tgggtaaacg gtaccgctcc
    20941 agcgaggaca gtatcctggc ccacatcttg gactatatca agtgcaatta taccgaggcg
    21001 ctcaccgtgc cggagatcgc ccgcttctgc cattgcagcg aatcttatgt caaccatctc
    21061 ttcaaaaaac gggtgggaat caatattaaa atgtacatca ataaaatccg gattgaagcg
    21121 gcccaagaag aattaacccg ctcggatcgg aa
//
LOCUS       DASVMB010000512        10167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_652411,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000512 DASVMB010000000
VERSION     DASVMB010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652411"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1653
                     /locus_tag="VF531_14440"
     CDS             133..1653
                     /locus_tag="VF531_14440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7715211.1"
                     /translation="MKRSFIIWLTLLVIILLSTTMPLNQSVSAASDYWPAADWRTSSP
                     EEQGIDSAKLYRMLAYIKENNIDIHSILIIRHGYLVLESYFAPYDKDTIHNLKSVSKS
                     YLSALVGIAFREKLLKGLDQKVADFFPEYFASGDDPRKKETTLRHLLTMTAGFNWAEN
                     SPVSNGLWHSQNWVKYAIEMPMNDNPGEKFTYSTALTHLMSAILTKTSGMSTQEFAEK
                     YLLDSLGIDVKLWRRDPQGICFGGSELFLTPRDMAKFGYLYLRQGIWNGKPIVPADWI
                     EQSVKGHVKTGNWGGFADYGYWWWLEPGSYAAEGWGGQRIVVVPDEDLVVVFTSADFN
                     QPRSLYEFFIKPAVKIGKIPANPKESAALAKLVAELGHPQAKPVLPLPEIASQISGKT
                     YQVEKNGFGITALRFDFKDGNQCLLTLDTPGGKLELPVGLDDLYRFSNEVSDVPVGFK
                     GWWVGAGPILEMNWLYLGEPFKYQGLFTFEGDEVRISVTVTPSNQFETVTGKAVVK"
     gene            1948..2697
                     /locus_tag="VF531_14445"
     CDS             1948..2697
                     /locus_tag="VF531_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715212.1"
                     /translation="MTEKKPEARRRRRKQEIEQFLAGRDWNDVFHIWKDTLDNLSITL
                     QPLFTEVWAHDPKYPVYHENAFSWWPAESIQELQLSLQRHLDHFPGSIGPLHRKEYYT
                     RKGELDTLDYLWKNREKAEKIAAILISASLFYRLKSSRRDYPRGHWPPISCVEMVFHL
                     ALSQWFEGEWGLPWHHACTRVLPDIRSDKDCKIDDLQAFIHYLAEEHAAVLSGFKPVA
                     ITFKPQRDPRIEQQLLEEYELERKNRDLSRN"
     gene            2825..3625
                     /locus_tag="VF531_14450"
     CDS             2825..3625
                     /locus_tag="VF531_14450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7715213.1"
                     /translation="MRTIYKCFPGGKHKVLTMSYDDGKLSDRKLVAIFNQYGIKGTFH
                     LNSGLIDGDPHRVPASEIHELYQGHEVAAHTLTHPTIARCPMEQVVQQILEDRKNLER
                     LVGYTVRGLSYPNGSYNQAIQSMLPHVGIEYSRVVGSSESFELPDNLFAWQATCHHKH
                     DLIRLGEEFAALHKTQYLYMMYVWGHSYEFDNDGNWDLIEQFCRNMNRREDVWYATNI
                     EIVDYLKAAANLKFSAAGDVVYNPAAQSVWLSVDGTIIEIKGGAQVRL"
     gene            3746..5020
                     /locus_tag="VF531_14455"
     CDS             3746..5020
                     /locus_tag="VF531_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715214.1"
                     /translation="MRRILIFLFMALMLVSSLIVWAESGESEIARADQLWANHFTQGM
                     TEKAIGVLGTIVEQEPDNYEALWRLGRCYQFLGEVSFNKERTVAIEKGLSFTGRAVKI
                     NDQGVNGHLWHALLVGSNLQDQRDPGGLGLVKQMHDELQTVVRLEPQNGIAHFALAQL
                     YHEAPGKPVGIGDKKKALTEAALAVKHMPSNNDAWIYYGYLEQENGDFAAARVAYARF
                     LDNTGSFKKYSVLVGEEEQPGQDYETLWHLGYFYEFYGRNCPVKKEKLAAFERGLSYT
                     EEALKLNPNGVNGHLYKALLTGDIGLEKGISKGLAMVKPMCDELQTVINLEPGNAIAH
                     YALGQLYWKAPGKPLSIGDKKKALEETALAVKYDPQNIEYWFYYGKIAADNKDYKTAK
                     TAFTKVLNFFGGQRDFKAEAQKELNRLGDVKK"
     gene            5185..5811
                     /locus_tag="VF531_14460"
     CDS             5185..5811
                     /locus_tag="VF531_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HEX7715215.1"
                     /translation="MKTVKSVKKIVWIAVLFGLSGCQSLEYQKLQIGDIIFQTSNSSQ
                     SQAIQAATHSKYSHLGMIYTENGKYLVFEAVQPVKLTPLKDWIKRGQDGHYVVKRLKN
                     AGEVLNKETLAKMKKIGLEYMGKNYDPYFEWSDQRIYCSELVWKIYKRALGVEIGKLQ
                     MFKEFDLSSPIVKAKLKERYGTDIPLRELVISPDQMFRSDLLQTIIEK"
     gene            6028..6195
                     /locus_tag="VF531_14465"
     CDS             6028..6195
                     /locus_tag="VF531_14465"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX7715216.1"
                     /translation="MAFHNHPSGEAEPSAWDRAITMKLKDIGEFLNIRLLDHIIIGER
                     GYSSLKEQGLL"
     gene            6311..7189
                     /locus_tag="VF531_14470"
     CDS             6311..7189
                     /locus_tag="VF531_14470"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX7715217.1"
                     /translation="MNKKALTKGLFIFGAGFIVMFVIGLIGGYVEPVPQAPLQQSLGA
                     VSRFEYNVKNVASTQMKVQGNGSVKVYEVDQKYQKVADLATETGRFSEDEQKIRAVVK
                     KHNALIQYEENTGLPGSRFLQLAIGVPPARFDAMVLEVKKIGALKSFNISKTDKTNEF
                     KELQGKRRSLEQTRASLLALKARSGGKIDEYINLENRILEIEQELQNLGVQLGDYDVT
                     NEFCTIKMTLSETAIRISPSIWFRIIKALEWAIKYYLLLVVILCVCSLTTLILVVVFD
                     KIKRLMRIESNKASEL"
     gene            complement(7338..7598)
                     /locus_tag="VF531_14475"
     CDS             complement(7338..7598)
                     /locus_tag="VF531_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX7715218.1"
                     /translation="MISKTHGDNISNTEITNISCYGIWILTGDKELFMSYENFPWFKD
                     VPVSQILNVEEPSPSHFYWPDLDVDLTTEIIEHPERFPMKYN"
     gene            complement(7614..7718)
                     /locus_tag="VF531_14480"
     CDS             complement(7614..7718)
                     /locus_tag="VF531_14480"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715219.1"
                     /translation="MICTDGEAKYWLEPDLELANNHHLSRQQLKKLNS"
     gene            8025..9113
                     /locus_tag="VF531_14485"
     CDS             8025..9113
                     /locus_tag="VF531_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7715220.1"
                     /translation="MNPNEIFVIYGNTPQSMVQELLAHIQPEREIDRNSRIGIKPNLV
                     VAKPAAGGATTSPELVAGVIEYLQAKGFQNICILEGSWVGAKTAEAFKACGYEEISRR
                     FGVPLIDLQKDSYREYDAGGMKLKICNRVTALDYLINIPVLKGHCQTKMTCALKNMKG
                     CIPNSEKRRFHTLGLHRPIACLNKAIRQDLIVVDGMTGDLNFEEGGHPVPMNRIMVAK
                     DPVLVDSYVAGLMGFELPEVPYIQMAEALGVGSAALEKAVIRELNAPQSIVEIGNFPK
                     FHEFAKYIDEREACSACYGSLVYALERMGNRGELDRLKAKLYIGQYYRGKCMDGIGIG
                     SCTSGFQYCIGGCPPKAKDIIDYLEKHF"
     gene            9383..>10167
                     /locus_tag="VF531_14490"
     CDS             9383..>10167
                     /locus_tag="VF531_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7715221.1"
                     /translation="MFVDTGYQNQDLCDLLMSGYVPKPVRNGAGATDPGPRDILRDLE
                     NPDMLVPPATDGGLLPNMKFSFSDTSMVLKPGGWSREVTARELPVATTMAGVNMRLTP
                     GGIREVHWHIASEWSYMLAGSARVTAVDQSGRNFIADIGPGDLWYFPPRLPHSLQGLK
                     DGCEFLLLFDDGNFSDLNTLSLSDLFAHYPKEVLAANFGVPMSDFNTLPSGQVYIYQG
                     TVPGPLESEGVQSPYGTVPQSFKHRLLAQKPITTAGGSVRIADT"
BASE COUNT         2824 a         2110 c         2552 g         2681 t
ORIGIN      
        1 gaatcttatt ttgtgatgag gaaaaggtat ctaaatatat ttctagaaat taatgccaat
       61 actggttttg aaatttttgc ggcttaaata taatgattaa tcgaccaata ttttgggtag
      121 gagtgatgga atgtgaaaag aagttttatc atttggttga cgctgttggt tatcattcta
      181 ttaagcacca cgatgccact caatcaaagc gtaagtgcgg catccgacta ttggccggcc
      241 gctgattggc ggacttccag tcccgaagaa caaggaattg actccgccaa actctaccgg
      301 atgttagcgt atattaaaga aaacaatatt gatattcata gcatacttat catccgtcat
      361 ggttatttgg ttttagagag ttacttcgct ccgtatgaca aggataccat acacaacctg
      421 aagtcggtct ccaaaagtta tctttcggca ctggtaggga tcgccttccg ggaaaagctg
      481 ctaaagggcc tcgatcagaa ggtggcggat ttttttccgg aatattttgc ttccggcgat
      541 gacccccgta aaaaggagac taccttacgg catcttttaa ccatgactgc cgggttcaat
      601 tgggctgaaa actcccccgt ctcgaatggt ttatggcata gtcagaattg ggtcaagtat
      661 gcgattgaga tgccgatgaa tgataatccg ggtgaaaaat tcacttatag tacggcgctt
      721 acgcatctga tgtcggcgat cttaaccaag accagcggga tgagtactca ggaatttgcc
      781 gaaaagtacc ttttggattc gttgggtatc gacgtcaagt tatggcggcg tgaccctcag
      841 ggaatttgtt tcggcggctc ggagcttttt cttacgccgc gggatatggc gaaattcggt
      901 tatctctatt tgagacaggg aatttggaac ggcaaaccga tcgtcccggc cgattggatt
      961 gaacagtccg ttaaggggca tgtaaaaacc ggaaactggg gcggatttgc cgattacgga
     1021 tattggtggt ggcttgagcc gggaagttat gcggcggaag gttggggcgg tcaacgaatt
     1081 gttgtggtac cggatgaaga tctagttgtt gtctttacca gcgctgattt taatcagccc
     1141 cgtagccttt acgaattctt tatcaaaccg gctgtgaaaa tcggtaaaat accggcgaat
     1201 ccgaaagaat cagctgcctt ggccaaattg gttgcggaat tagggcatcc gcaggccaaa
     1261 ccggttttac cgctgccgga gatcgccagt cagatatcgg gtaaaacata tcaggttgaa
     1321 aagaacggtt tcgggataac cgcgctccgt tttgatttca aagatggcaa ccaatgcttg
     1381 cttacacttg atacaccagg cggcaagctg gagttgcccg tagggcttga tgatttatac
     1441 cgtttcagca atgaagtaag tgatgtcccg gtcggtttta aaggctggtg ggttggagct
     1501 ggacccatct tggaaatgaa ctggctttac ctgggggaac cctttaaata ccaggggtta
     1561 tttactttcg agggagatga ggttagaatt tcagtaacag ttacccctag taaccaattc
     1621 gaaacagtta ccggaaaagc ggtagtcaaa tgagtttgtt ttcggtacgg cactatgagg
     1681 ttcaatcatt tggacctctt ttttgttttc cgattttttc aggagatgag gggaggttct
     1741 gggttttatc aaaatgaagg gcaaaaaggg aaaaaaatat aatttctatg acgggacagg
     1801 ccgattttat gatcagcgga ttgatcatgc cgggatttga cgttccgcgc ttcacggatc
     1861 tgtagaactc ggagtttccc agatttgaat atctcttagg agaagataga ctgggcagat
     1921 tgttaaaaaa cgccttagga gtttgccatg accgaaaaaa aacctgaagc caggcgaaga
     1981 cggcgcaaac aagagatcga gcaattcctt gccgggagag actggaacga tgtctttcat
     2041 atctggaaag acacccttga taatctatcc ataactcttc agccgctctt taccgaagta
     2101 tgggcgcatg atccgaaata tccggtatat catgaaaacg ccttttcatg gtggccggca
     2161 gagtccattc aagagctgca attaagccta caacgtcatc tcgatcattt tcccggctcc
     2221 atcggcccgc tgcatcgcaa ggaatactat acgcgcaaag gcgagctgga cactttggat
     2281 tatctctgga aaaaccggga aaaggctgaa aagatagcgg caatcctgat ttcagcgtcg
     2341 cttttttacc gcctcaaaag cagccgtaga gattacccgc gtggccactg gccgccaatc
     2401 agttgtgtag agatggtttt ccatctggcg ctttcccaat ggtttgaggg agaatgggga
     2461 cttccctggc atcacgcatg taccagagtt ttaccggata tccgcagtga taaggattgt
     2521 aaaattgatg acttacaagc ttttatacat tatctcgcgg aagaacacgc tgcggtactc
     2581 tcgggtttta aaccggttgc cattacattt aagccgcaac gggatccccg catcgagcag
     2641 caattgcttg aggaatatga gttggaacgt aaaaatcgtg acctgagtcg aaactagcgg
     2701 ggtgaacttg gtttagtcag caattcaccg tgccatatat tgaaggtcta acattcgaaa
     2761 gatattgctg gtactatgtt ggccttggcc aaatcaaaaa caatagatat cggagatgat
     2821 attgatgcgt acgatttata aatgttttcc aggcggcaaa cataaggtat tgaccatgag
     2881 ttatgatgac ggcaagctgt cagatagaaa gctggttgcc atattcaatc aatacggtat
     2941 caaagggaca tttcatttaa actcgggctt aatcgatggc gatccccatc gcgtaccggc
     3001 atccgagatt catgagctct atcaaggtca cgaagtagct gcccatacgc tgactcatcc
     3061 tacgattgcc agatgcccca tggaacaggt ggtccagcaa attttggagg accgtaaaaa
     3121 tcttgaacgc ctggtgggtt atacggtcag aggattgtcc tatcccaacg gctcatataa
     3181 ccaggctatc caaagcatgc tgccgcatgt cggcattgaa tattcaagag tcgtcggcag
     3241 cagcgagagt ttcgaattac cggacaatct atttgcctgg caggccactt gccatcataa
     3301 acatgacttg ataaggctgg gtgaagaatt tgcggcgctc cataaaacac aatatcttta
     3361 tatgatgtat gtttggggcc acagttacga attcgacaac gacggcaact gggacctcat
     3421 cgaacaattt tgccgcaata tgaatcgtcg ggaagatgtc tggtatgcca caaatattga
     3481 aattgtggac tatttaaaag ccgccgccaa tttgaaattc tccgcagccg gcgatgtggt
     3541 gtataatccg gcggcacaat ccgtatggct cagcgttgac ggcacgatta ttgagatcaa
     3601 aggcggggct caggttcgcc tatagtcaag aaaataaacc tcacttgttc tgcttgggaa
     3661 caacccgagc gccggatgaa accctgaagg ttaaaagtct atctaaaact tgtaatctcg
     3721 attattccat aaggagtaaa agccaatgcg acgaattttg atcttcctat tcatggcttt
     3781 aatgctggta tctagcctta tcgtttgggc cgagagtggt gaaagcgaga ttgccagggc
     3841 ggatcagctt tgggcaaatc atttcaccca agggatgacc gagaaggcga ttggggtgct
     3901 gggaaccata gtggagcaag aacccgataa ttatgaggcg ctttggcgat tgggccgctg
     3961 ttatcaattt ttgggagagg tttcgtttaa taaagagagg accgtcgcta ttgaaaaagg
     4021 attgtctttt accgggcgag cggtaaagat taatgatcag ggggttaatg gacatctttg
     4081 gcatgccctc ttggttggca gcaatttaca ggatcaacgc gatccgggtg gcctcggtct
     4141 ggtcaagcag atgcatgatg aattgcaaac ggtcgtgcgg ttggaaccgc aaaacggtat
     4201 cgctcatttt gcactggctc agctctatca tgaggctccg gggaagcctg tcggtattgg
     4261 cgataagaaa aaagcactaa ccgaggccgc cttggcggta aagcatatgc catcgaataa
     4321 tgacgcctgg atttactatg gttatctgga acaggagaat ggtgatttcg ccgccgcccg
     4381 ggttgcatac gctagatttc tcgataatac cggtagcttc aaaaaatatt cggtgttagt
     4441 aggggaggaa gaacagccgg ggcaagatta tgagacgctc tggcatttag ggtactttta
     4501 cgaattttac ggccggaatt gcccggttaa gaaagaaaaa ctggctgcct ttgagagagg
     4561 gctatcatat accgaagaag ccctgaaact aaatccaaac ggagttaacg ggcatcttta
     4621 caaggcactt ttaacgggcg atatcggcct ggaaaaaggc atttcaaagg gtttggcgat
     4681 ggtaaaaccg atgtgcgatg agttacagac agtaataaac cttgaacccg gaaatgccat
     4741 tgctcattat gcattaggcc agctatactg gaaagcgccg ggtaagccgc tgagtatcgg
     4801 cgataaaaag aaagcgcttg aagaaacggc attggcggtg aaatatgatc ctcaaaacat
     4861 tgaatactgg ttttactacg gtaaaatagc cgcagataac aaggattaca aaacggcgaa
     4921 gacagcgttt accaaggtac tgaacttttt tggggggcag cgcgatttca aggcggaagc
     4981 ccagaaagaa ttgaaccggt tgggggatgt caaaaagtag attatgcagc taaaatcagt
     5041 tgacatttga gaactagaaa aagttctttg aatttgccat accttttaat attaactctc
     5101 ttaaaggcat accgggattt gctcaaattt gcgggaacta tcgataccag gacattccga
     5161 aagcgatggg aaagtagtgg aataatgaaa acagtgaaga gtgttaaaaa aatagtatgg
     5221 atagcggtgt tattcggact atcaggctgt caaagtttag agtatcaaaa gttacaaatc
     5281 ggcgatatca tttttcaaac atcaaattcg tcacagagtc aagcgatcca ggctgcaact
     5341 cattcaaaat attctcacct tggcatgatt tatactgaaa acggtaagta cttagttttt
     5401 gaagcagttc aacctgttaa attaacacct ttaaaagatt ggattaagag aggccaagac
     5461 ggtcattacg ttgttaagcg cttaaagaat gccggtgaag ttttaaataa agagactttg
     5521 gccaagatga aaaaaatcgg cctggagtat atgggtaaaa actatgatcc gtattttgaa
     5581 tggtcggacc agcgaattta ctgttccgaa ctggtttgga agatttacaa gagggcattg
     5641 ggagttgaga ttggaaaact tcagatgttt aaagaatttg atttatccag ccccattgta
     5701 aaagcaaaac taaaggaacg ttacggcact gatattccgc tccgggaatt ggtaatatca
     5761 ccggatcaaa tgttcaggtc ggatttattg caaaccatca ttgaaaaata agaataagta
     5821 gcggatggaa ggctgtatac tgagactatt tcaaaagaag caaccttgga gtctgtcaat
     5881 gcgccggtta ctttttatct ttctgttttt tagataagcc acaaaatcct gtatctcttt
     5941 cctagactaa tccggaagtc cggataaagc ctcaccgaga aagagctgac cctgtgcttc
     6001 aacacgtccc aaaagatata tgccatcatc gcttttcaca accaccccag cggcgaagcg
     6061 gaacctagcg cctgggatcg ggcgatcacc atgaaactta aggatatcgg ggagtttctg
     6121 aatatcagat tgctggatca cattattatt ggggaaaggg gctattcaag cctgaaggaa
     6181 cagggtctgt tgtagtttat cggggctgtc tcaaaagagg cagccttggt tttcattttt
     6241 gcaggaaaaa taaaccagta ttagaactat atattatctt tcttttcaaa gcaggtaaag
     6301 gagtgatctt atgaataaaa aagccttgac caagggttta ttcatcttcg gcgcgggttt
     6361 tatcgtcatg ttcgttattg ggttgatagg cggctacgtt gagccggtcc ctcaagcccc
     6421 gctgcaacaa tcattgggtg cggtaagccg gtttgagtat aatgtgaaaa atgtggccag
     6481 cactcaaatg aaggtccagg gaaacggttc tgtgaaggtt tatgaagtgg atcaaaaata
     6541 tcagaaagta gccgacctgg ctaccgagac cggccggttt tccgaagacg agcagaagat
     6601 tcgggctgtc gtcaaaaaac ataatgcctt gattcagtat gaagagaata cgggcctgcc
     6661 cggaagccgc tttctgcaac tggccattgg ggtgccgccg gcccggtttg acgcgatggt
     6721 tcttgaggtg aaaaagatcg gcgcgcttaa gtccttcaac atcagtaaga ccgataagac
     6781 aaatgaattc aaggaattgc agggaaagcg ccgttcattg gaacagaccc gcgcatcgct
     6841 actggctctc aaggccagaa gcggcgggaa aatcgatgaa tacatcaact tggaaaaccg
     6901 gatactggaa atcgagcagg agctgcaaaa tctgggagtg caattgggcg actacgatgt
     6961 gaccaacgag ttttgcacca tcaagatgac gctttccgag acggccatcc ggatcagccc
     7021 ctcgatttgg ttccggatca tcaaggccct ggaatgggcg atcaagtatt atctgctatt
     7081 ggtggtcatc ttgtgtgtct gtagtttaac aaccctgatc ctggtggtcg tttttgataa
     7141 aatcaagaga ttaatgagga ttgagagcaa caaagcatcg gaattataat aatcataaaa
     7201 ggcttatcct aaagttgtta ccttttggga taggcctttt gtcatacttc ggtaatttgg
     7261 cgtttagcct ctacccatgg gactaatttg actttaccgc agtatatagg gatagacaga
     7321 ctgaagattt ttggacatca attgtatttc atagggaacc gttctgggtg ctcaataatt
     7381 tcagtggtaa gatctacatc gagatccggc caataaaagt gggaaggaga tggttcttca
     7441 acattcagta tttgactcac cggaacgtct ttgaaccaag ggaaattttc ataggacatg
     7501 aataattcct tatctcccgt aagaatccag attccgtaac acgagatatt tgttatttca
     7561 gtattggaaa tgttgtcccc atgcgttttt gatatcattg taatgctcct ttattaactg
     7621 ttcaatttct ttagttgttg acgtgataga tggtggttgt tcgctagttc aagatccggc
     7681 tccaaccagt atttggcttc accatctgta caaatcacat gtacatgtat tctgggttct
     7741 tctcgtaaaa gacaaagaac cggtatccct tttccttaaa tatagtagga ctcatagatt
     7801 ttcacatcca tcccaagaag gcaattttcc cagaatcctt gaattgggat aatttcatga
     7861 taacatagaa attgatgaaa agatatatcc ctcattggtt tggctggttc gtatatatag
     7921 cataaaatga aaaatcagaa gcaccaaagc catcactata ttctatctaa atttggtata
     7981 attgtttata tttaaaagac tttttacctg gagctgagat ctctatgaac cccaacgaga
     8041 ttttcgtaat ctacggcaat actccccaat cgatggtaca ggaactgcta gctcatattc
     8101 aacccgagcg ggagatcgac cggaactccc ggatcgggat caagcccaat ttggtggtgg
     8161 ctaagcctgc tgccggcgga gcgactactt cgcccgaact ggtcgccgga gttatcgagt
     8221 acctgcaagc caagggcttt caaaacattt gcattttaga aggttcctgg gtgggagcca
     8281 agactgcgga ggcttttaag gcatgcggat atgaagaaat ttcgagacga tttggagtcc
     8341 ctctcatcga cctgcaaaaa gacagctaca gggagtatga tgcgggagga atgaaactta
     8401 agatttgtaa cagggtgacg gctctcgact acttgatcaa tattccggtg ctcaaaggcc
     8461 actgccaaac caagatgacc tgtgcgctga agaatatgaa ggggtgcatt cccaactctg
     8521 aaaagaggag atttcatacc ttgggactgc atcggcctat tgcctgcctg aacaaggcga
     8581 tccgacaaga ccttattgtt gttgatggga tgacgggcga tttgaacttt gaagagggcg
     8641 gccacccggt cccgatgaac cgcatcatgg tagccaaaga tccggtgctg gtggatagct
     8701 atgttgccgg cctgatgggc tttgaactcc cggaggttcc ctatattcag atggccgagg
     8761 ccctcggcgt cgggagcgct gctctggaaa aggccgtaat ccgggagctc aacgcccccc
     8821 aaagtatagt ggagattggg aatttcccga aattccacga gtttgcgaaa tacattgatg
     8881 aacgtgaggc gtgttcggcc tgttacggaa gcctggtata cgcgctggaa agaatgggaa
     8941 accgaggtga gttggacagg ttgaaggcta agttatatat tggccagtat taccggggca
     9001 agtgcatgga cgggatcggc atcggctcgt gtacctcagg ctttcaatac tgtattgggg
     9061 gctgtcctcc aaaggccaaa gatatcattg actatttaga gaaacatttt taaagatcac
     9121 aggcttccaa aaaatataca cctctgtcca aagatgctga atatcttctt actttgaaag
     9181 agtgaactca tttatgtcga aaatgagttc ttttttattt ccatgtatcc tctccgcaca
     9241 ttgccgtatc ataaagtagc ttgaaacatg aggcgggtgt atggacatat ttcctatgtc
     9301 catttttttc atagaatatt cagacatacc taaggcgctc gaacctgtgc ttaatatagg
     9361 gaattcgaaa tgaactggag gaatttttgt ggatactgga tatcaaaatc aagacttatg
     9421 cgatctgctt atgtccggtt atgtgccgaa gccggtaaga aacggcgccg gggcgaccga
     9481 tcccgggccg cgcgatattc tccgggatct tgagaatccc gatatgctcg ttccgcctgc
     9541 tacggatgga ggcttgctcc cgaatatgaa attttccttc tccgacacca gcatggtatt
     9601 aaaaccggga ggatggtccc gggaggtcac agccagggaa ctgccggttg cgaccacaat
     9661 ggcgggcgtc aatatgcgct tgacccctgg tggtatacgc gaggttcact ggcatatcgc
     9721 ctcggaatgg tcctacatgt tggcggggag tgcgcgcgtc actgcggtcg atcagagcgg
     9781 acggaatttc attgccgata tcggtccggg agacctttgg tattttcccc cgcgccttcc
     9841 ccattcgctt cagggcttga aagacggctg tgaatttctg ctcctcttcg acgacggaaa
     9901 cttctccgac cttaatacct tgtccctctc cgacttgttt gcgcattatc ccaaagaggt
     9961 gctggcagcc aatttcggcg tgccaatgag tgattttaac actcttcctt caggacaggt
    10021 ctatatctat cagggcactg ttccgggtcc tttagagagt gagggggttc agtcccccta
    10081 tggaacagtt ccccaaagtt tcaagcatcg gttattggcg caaaagccaa taacaacagc
    10141 tggcggaagc gtgcgaatcg cggatac
//
LOCUS       DASVMB010000513         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_138744,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000513 DASVMB010000000
VERSION     DASVMB010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138744"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VF531_14495"
     CDS             <1..1044
                     /locus_tag="VF531_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715222.1"
                     /translation="LLTYAWNGARFLRLDAIGFIWKELGTTCMHLKQTHTVVKLMRAV
                     LDEIFPGTFIITETNVPHADNISYFGNSDEAHLVYQFPLPPLTMFSLQSGDTTKLTKW
                     AQSLENTPLHQNNTYFNFLASHDGVGVRPLEGILGDEEKKLLFDNVIQNGGRISYAVS
                     KDGSVNPYELNISYMDGITHPSKDKDERVNRFMAAQAIMLSMQGVPGIYYHSLLGSES
                     WYEGADSSGINRRINREKLDYEKLTGDLKDRSSLRYGVFTAYKEILKIRAKHSAFSPY
                     ASQKVLNLSPSFFAVERYNESTQERIIAIINVTDKQVKLDQAIKGTDLFAANQTGGII
                     TKLEPYQIAWILV"
     gene            1100..>1815
                     /locus_tag="VF531_14500"
     CDS             1100..>1815
                     /locus_tag="VF531_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX7715223.1"
                     /translation="MGQLLVLEQPGKVGYEDYEERPLESNEVRIKTLFTGISAGTELT
                     SYRGTSPFFNKKWDPGKRIFEKQTESSWSYPLKQWGYEEVGEVVEIGSSVKQVQLGDI
                     IFGTWGHKSTHIAEEAFAADRKLLEDLDPVCGIFSHIGAIGINAILDANIHVSETVAV
                     FGQGVPGQIVTQLAKLNGAKVIAVDLDDTRLAVAKKFGADLVLNSQKCDASGEIKEIT
                     KGLGADVAIEISGSPVALHEA"
BASE COUNT          525 a          358 c          444 g          488 t
ORIGIN      
        1 cttctaactt atgcgtggaa cggcgcccgt ttcctccgtt tggatgccat tggttttatt
       61 tggaaggaac ttggaacaac ctgtatgcac cttaagcaaa cccatacggt tgtaaaatta
      121 atgcgggccg tgttggatga aatattcccg ggtacattta ttattacaga aacgaatgtc
      181 cctcatgccg acaatatcag ttatttcggt aatagcgacg aggctcattt ggtttatcaa
      241 tttcctttgc ctccgttaac gatgttttcg ttgcaaagcg gtgataccac taagcttaca
      301 aagtgggctc agtcattaga aaatactcct ttacatcaaa ataataccta ttttaatttt
      361 ctggcttccc atgatggcgt tggcgtccga ccattagaag gaatactcgg cgatgaggaa
      421 aaaaagctcc tgtttgacaa tgtaattcaa aatggtggaa gaatcagtta tgctgtaagt
      481 aaagatggca gtgtgaaccc ttatgaattg aatatcagct atatggatgg gattacccat
      541 ccatccaagg acaaagatga aagagtcaat agattcatgg cggcccaagc cattatgctt
      601 tctatgcaag gagtacccgg tatttattat cacagtttat tgggatcgga aagctggtac
      661 gaaggggctg attctagcgg aattaaccgc aggatcaacc gggaaaaatt ggactatgaa
      721 aaactgacag gcgatctgaa agatcgatcc tcccttcggt atggggtatt tacggcgtat
      781 aaggaaattc taaaaatcag ggcaaagcac agcgctttct caccttatgc cagtcaaaag
      841 gtcttaaatt tatcgccttc gtttttcgcg gttgagagat acaatgagtc tactcaagaa
      901 cgaatcattg cgattatcaa tgtcacggat aagcaagtca agctggatca agcgattaaa
      961 gggaccgatc ttttcgctgc aaatcaaacc ggaggaatca tcaccaaact tgaaccctac
     1021 caaatcgcat ggatattggt ttgatttttg agatacccct cgaaaagggt ttctaatact
     1081 acaattggga gagattttca tgggtcaatt attagttttg gagcagccgg ggaaggtcgg
     1141 ctatgaggat tatgaggaaa ggccgcttga gagcaatgaa gtccgcatta aaacattatt
     1201 tactggtatc agtgccggaa ccgagttgac ctcctatcga ggcaccagtc catttttcaa
     1261 taaaaagtgg gatcccggca aacggatctt tgaaaaacag accgaatctt cctggagtta
     1321 tccgctaaaa caatggggtt atgaagaagt tggcgaggtt gtcgaaattg gcagcagtgt
     1381 gaaacaggtt cagttaggcg atatcatctt tggtacctgg gggcataaaa gtacccatat
     1441 tgccgaagaa gctttcgcgg ctgaccgcaa gctactggag gatttagatc cggtgtgcgg
     1501 aatattttcg catatcgggg cgatcggcat caatgcgatc ctggatgcca atattcacgt
     1561 cagtgaaacc gtagctgttt ttgggcaggg cgtgccggga caaatcgtaa ctcagctggc
     1621 gaaattgaat ggggccaaag taatcgccgt tgatttggat gatacccgat tggcggttgc
     1681 taagaaattt ggagcggatc tggtcctaaa ctcccaaaaa tgtgatgctt caggagaaat
     1741 taaggagatt accaaaggtc tgggagccga tgtggctatt gaaatatccg gttcccccgt
     1801 tgcattgcat gaagc
//
LOCUS       DASVMB010000514         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_360321,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000514 DASVMB010000000
VERSION     DASVMB010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360321"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="VF531_14505"
     CDS             complement(<1..2201)
                     /locus_tag="VF531_14505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7715224.1"
                     /translation="MNVYIFLPMVALLVNNFTWTYILAQNRKNPVNRAYLSFSGVISL
                     WMFLNLILWLPLDERWMLLLLRYNAAVWLPLGFLLLNFTYALLHIENDWIYHGMALLT
                     IAAVILSATTDLVGYGCQKFYWGPNQYPGPLYLPVTLGVVGGPGFYALYLILNRYRII
                     HDQLIKSQLRLIMYGAFAALVLGFFTDVIMPNFFNRPDFLPLASTAGVILSVLVFWAV
                     VRYNFLSIGIEDIAADLFAKVQDGVIIYNNRQEIVQFNQAAAELLGFTTPPAPGTMDR
                     YIDHYRFTEEFTDFETTPSCQPTKLVSLSKVPIRYNKQAIGEMLIIRDITAYKNAIDD
                     LNRHRNQLEKLVSERTQLLTESNEQLRHEIAERAQQEQFLKRQEQLLQTAIAGLRESE
                     ARYRALAENTIDLICETSADGQYLYASPNYKEIYGFDPDELLGRNIFTGVHPDDLPQA
                     REVFKRSFKTFSSGRAVYRFQNQKGECAWYESIGKPYIAPNGEVRTVMITRDITARKK
                     MEEEIIKANKLESVGILAGGLAHDFNNILSIIWGNISLAKLFLKPQDNAYPKLVKAEQ
                     AINRARELTQQLLTFAKGGAPVKATIILTKLVTESVNMSLHGSAVQYRLTASHDLWPV
                     DADAGQLNQVFNNLVINAVQAMPEGGTLTIDLTNLALEEEPALPLDAGNYVRITFQDT
                     GVGISDADLRKAFDPYFTTKPQGTGLGLTTAYSIIKRHNGCIVLESEPGRGT"
     gene            complement(2430..3821)
                     /gene="asnS"
                     /locus_tag="VF531_14510"
     CDS             complement(2430..3821)
                     /gene="asnS"
                     /locus_tag="VF531_14510"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX7715225.1"
                     /translation="MEKVTVRELFRETAKHLGTTIQVSGWVRTIRDSKTFGFIELNDG
                     SFFKNLQIVFESSLDNFSEVAKLNISSAIIVEGELVESPGAKQPFELKATRVEIAGEA
                     ALDYPLQKKRHTFEFLRSIAHLRPRSNTFAAVFRLRSLAAYAIHRFFQERSFVYVHTP
                     LITGSDCEGAGEMFQVTTLDLNNLPRDAQGKIDFSRDFFGRPTNLTVSGQLEVETYCL
                     AFRNVYTFGPTFRAENSNTPRHAAEFWMIEPEMAFADLRDDMLLAEEMLKYLINFCLE
                     QAPEEMEFFNTFIDKTLLERLEHIVKSDFGAITYTEAIKILEKENSRFEYPVAWGKDL
                     QTEHERFLTEQVFQKPVFVIDYPKEIKSFYMRMNDDLKTVAAMDLLVPGVGEIIGGSQ
                     REERLEVLEQRMAELKLNPKDYWWYLDLRKYGGNKHAGFGLGFERAIMYLSGMGNIRD
                     VISFPRTAGTAEF"
     gene            complement(3848..4870)
                     /gene="asnA"
                     /locus_tag="VF531_14515"
     CDS             complement(3848..4870)
                     /gene="asnA"
                     /locus_tag="VF531_14515"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEX7715226.1"
                     /translation="MSPVATQIQIPAGYHPRLTVFETETAIKQLKDRFEQALAKELNL
                     LRVSAPLFVKPESGLNDNLNGIERPVSFPVKGLENSTVEVVQSLAKWKRLALKRYGMK
                     NGEGLYTDMNAIRPDEDLDNLHSVYVDQWDWEKVLANDSRNIETLQSIVRRIYKVFLE
                     AESYICGLYPALSRTIPPEITFITTQELEDRYPQLKPREREDRIAAEKGAVFVMQIGG
                     VLRSGQKHDGRAPDYDDWSLNGDIIFWYPLLERAFEVSSMGIRVNAAVLADQLQQAHC
                     AARRDLPFHRALLAGELPQTIGGGIGQSRICMFFLQKAHIGEVQASVWPDEMLTACEA
                     EQIHLL"
     gene            5066..5728
                     /locus_tag="VF531_14520"
     CDS             5066..5728
                     /locus_tag="VF531_14520"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX7715227.1"
                     /translation="MRLQRKLRWEEFILSNVELSQIESVSVQQAMSTVPRIKFVPSEY
                     QHLADQDRPLPLAYGQTLSQPLIVARMTELLELTAIDKVLEIGTGSGYQTAILAELAG
                     TVFTVELIQPLAEQARRILVELGYDNIQFRIDNGFYGWPEKAPFDAVIVTAAATEIPG
                     SLFAQLKPGGRMVLPVGFPGEIQTLWRITKSIYGAKMERFDPVRFVPLQNPEPSSTQP
                     VE"
BASE COUNT         1476 a         1502 c         1365 g         1385 t
ORIGIN      
        1 gttccccggc cgggctcaga ttccagtaca atacagccgt tatgtctttt aatgatggaa
       61 taagccgtgg tcagacccaa accggtccct tgcggtttgg tggtaaaata gggatcaaag
      121 gccttacgga ggtcggcatc ggaaatcccg actcccgtat cctgaaatgt gatccggaca
      181 tagttccctg catctagggg taatgccggt tcttcctcca atgccagatt ggtaaggtcg
      241 attgtgagag ttcctccctc cggcatggcc tggacggcgt tgattaccag attattaaag
      301 acctgattta gttggccggc gtccgcatcc accggccaca agtcatgaga cgcagtcagc
      361 cggtattgaa ctgccgaacc gtggagagac atgtttaccg actccgtaac cagctttgta
      421 aggataatgg tcgccttgac cggtgcgccc cctttggcga aagtcaacag ttgctgggtt
      481 aattcccggg cgcggttgat ggcttgctcg gctttgacta attttggata ggcattgtct
      541 tgtggtttta gaaacagttt ggcgagggag atattgcccc agatgatgct aagaatatta
      601 ttaaaatcat gggccagtcc tccggcaaga atccccaccg actccaactt gttcgcctta
      661 ataatctcct cttccatctt tttgcgggcg gtaatatcac gggtgatcat caccgtccgg
      721 acctcgccgt tgggggcgat atacggcttg ccgatacttt cataccaagc gcactcccct
      781 ttttgatttt gaaacctgta aacggcacgg cccgaagaga aggttttgaa ggatcgctta
      841 aaaacctccc tggcttgggg taaatcatcg ggatgaaccc cggtgaagat attccgaccc
      901 aacagttcat cgggatcaaa gccgtaaatt tccttataat tgggactggc atataagtac
      961 tggccgtctg ccgaggtctc acagatcagg tcgatcgtat tctctgccag agcacgatag
     1021 cgcgcctcac tctcgcgtag gccggcaatg gctgtttgca gcaactgttc ctgccttttc
     1081 agaaactgct cctgttgggc gcgttcggca atctcatggc gcaactgctc attgctctcg
     1141 gttaacagct gggtacgttc ggaaaccagc ttttccagtt ggttgcggtg acgattcaga
     1201 tcatcgatgg cattcttgta ggcggtaata tcccgaataa tgagcatctc ccctatggcc
     1261 tgtttattat aacgtatcgg taccttggat agggaaacca gcttcgttgg ctgacaactc
     1321 ggggtagttt caaaatcggt aaactcctcg gtaaaacgat aatggtcgat ataacgatcc
     1381 attgtcccgg gagccggagg agtagtaaat cccaggagtt ccgcagcggc ttgattgaat
     1441 tgtacgatct cctgccggtt gttataaatg atcaccccgt cttgaacctt ggcaaaaaga
     1501 tcagccgcga tatcctcgat ccctatggat aaaaaattat accttacgac cgcccaaaag
     1561 actaaaaccg agaggatgac cccggcagtg gaagctagcg gtaaaaaatc cggcctgttg
     1621 aagaagttgg gcatgattac atcggtgaaa aaacccagga cgagcgcagc gaaagcccca
     1681 tacatgatca atcggagctg acttttaatg agttgatcgt ggatgatacg gtaacgattc
     1741 aggattaaat acagggcgta aaaaccgggg ccccctacta ctcccagagt aaccggaaga
     1801 tataacggtc cgggatactg gttgggaccc caatagaact tttggcagcc ataacccacc
     1861 agatcggtag tggcactcaa gatcaccgcc gcaatcgtca aaagagccat tccatggtat
     1921 atccaatcat tctctatatg tagcagggca tatgtaaaat taaggagcaa aaaacccaat
     1981 ggtagccaga ctgcggcatt ataccgcaat aacaacagca tccatcgttc atccagtggt
     2041 aaccagagga taagattcaa gaacatccac aaggatatta cgcccgaaaa ggaaagataa
     2101 gctcggttca cggggttctt gcggttttgg gccaggatat atgtccaggt gaagttgttt
     2161 accaacaggg cgaccatggg taaaaatatg taaacgttca tgatgaaaca acttcctatc
     2221 cgatactgga atggtaggcg ctgctagaca aaaaagcgca cctacgtaaa cagtctaaac
     2281 tctctttata tttcgatatt tatccataaa tacctgccgg tttcttacaa agaaggaatt
     2341 ataataaaag gaaaaaaaag actgcatccg gttggcccga acgcagtctg gaaatgatca
     2401 tgtcaatcac gccgtatacg gcaaaaagtt taaaattccg ccgttccggc ggtgcggggg
     2461 aatgagatca catcccggat attccccatt ccggaaagat acatgatcgc ccgctcaaat
     2521 ccgagtccga agccggcatg tttgttcccg ccgtatttac gaagatcaag ataccaccag
     2581 tagtctttgg gattcaattt cagttcggcc attctttgct caagcacctc cagccgttcc
     2641 tcccgctgac tgccgccgat gatttcgccc acccccggaa ccagaagatc catggcggca
     2701 acggtcttta gatcatcgtt cattctcata taaaaggact taatctcctt gggataatcg
     2761 atgacaaaga ccggtttttg gaatacctgt tccgtcagaa accgctcgtg ttcggtctga
     2821 agatccttac cccacgccac gggatactca aaccgggagt tttctttttc cagaatcttg
     2881 atggcttcag tataggtaat cgccccaaag tccgacttta caatatgctc caaacgttct
     2941 agaagcgtct tatcaataaa agtattgaag aattccattt cttcaggggc ttgttccagg
     3001 cagaaattga tgagatactt caacatctcc tccgccagga gcatgtcgtc acggagatcc
     3061 gcgaaggcca tttccggctc gatcatccaa aactccgccg catgccgtgg agtattggag
     3121 ttttccgccc gaaacgttgg accgaaggta taaacattcc ggaacgccag acaataggtc
     3181 tccacttcca actggccgct gaccgtcaga ttggtggggc ggccgaagaa gtcgcgggaa
     3241 aaatcgatct ttccctgggc atcccgggga aggttattca gatccaaggt ggtcacctgg
     3301 aacatctctc ccgccccttc gcaatcactc ccggtaatca acggagtatg aacatacacg
     3361 aaactacgtt cctgaaagaa tcgatgaatg gcataagccg ccagggatcg aagccggaaa
     3421 accgcagcaa acgtattgga acgcggcctc agatgggcaa tagatcgtaa aaactcaaaa
     3481 gtatgacgtt tcttttgaag cggataatcc agggccgctt ccccggcgat ctcgacccga
     3541 gtcgccttta actcaaaggg ctgtttggcc ccgggacttt ccaccaactc gccttcgaca
     3601 ataatcgccg aactgatatt cagtttcgcc acttcgctga aattgtccaa actggattcg
     3661 aacacgattt gcaggttttt aaagaaactg ccgtcattta gctcgatgaa accaaatgtt
     3721 ttggaatcgc ggatggtgcg cacccacccc gaaacctgta tcgtagtgcc caaatgcttc
     3781 gctgtctcac gaaaaagctc tcggaccgtc actttttcca ttaccgaaac ctccattcaa
     3841 agataaacta taatagatgg atctgctctg cctcacaagc cgtcagcatc tcatccggcc
     3901 agaccgaggc ctgaacctcc ccgatatggg ctttctgcaa gaagaacata cagatccggg
     3961 attgaccgat tccgccgcca attgtctggg ggagctcacc ggcgagtaga gcccggtgga
     4021 acggcagatc acggcgggct gcacaatggg cttgctgcaa ctgatcggcc aatactgccg
     4081 cattcaccct aattcccatg gaggagactt caaaagccct ttcaagcagg ggataccaaa
     4141 agatgatgtc cccgtttaaa ctccagtcat cataatccgg agccctgccg tcatgcttct
     4201 ggccggaacg aagtacgccc ccaatctgca tcacaaagac cgcacctttt tccgccgcga
     4261 tccgatcctc acgttctcgt ggcttaagtt gcgggtagcg atcctccagt tcctgggtgg
     4321 tgatgaaggt gatctccggg ggaatggttc tggatagtgc cggatataga ccacatatgt
     4381 agctttccgc ctccaagaac actttataaa tccggcggac gatactctgt aaagtctcga
     4441 tattccggga gtcgttcgcc agaaccttct cccagtccca ttggtccaca tagactgaat
     4501 ggagattatc cagatcctca tcgggacgaa tcgcgttcat atcggtgtac agtccttcgc
     4561 cgttcttcat gccgtaacgt ttcaaggcca gacgtttcca tttggccaat gactggacta
     4621 cttccacggt actattctcc aaacctttga ccgggaagga taccgggcgc tctatcccat
     4681 tcaaattgtc gttcaatccg ctttccggtt tgacgaatag cggcgctgaa acccgaagca
     4741 gattcaactc cttggccaat gcctgttcaa aacggtcttt caactgtttg atagccgtct
     4801 cggtttcgaa aacggtcaac ctggggtgat aacccgcagg aatttggatt tgcgtagcta
     4861 caggactcat ccatctacct cgtttcttat caatcaatta tctcccctgc acaactcaga
     4921 aagaataaaa aaggggtggt agtaggttat aaacgtcaga tacagattcg ctacccgtcc
     4981 ggaatctcct gccttaacgg gccaaccggg gttgataaat caaaaccgga aactttggaa
     5041 tactatcaag ggtccgtatt tccgagtgcg cctgcaacga aaactccgat gggaggaatt
     5101 cattctgtcc aacgttgaat tatcccaaat cgaatccgtc tccgtgcaac aagcgatgtc
     5161 aaccgtaccc cgtatcaagt ttgtcccgtc ggagtaccaa catttggcgg atcaagatcg
     5221 ccctttgccg ctggcctatg gccaaacgct ctcccaaccc ctgattgtgg cccgaatgac
     5281 cgaactcctg gagcttacag cgatcgacaa agttctggag atcggaaccg gttccggtta
     5341 ccaaaccgca atcctggctg aacttgccgg aaccgttttt actgtggaac tcatccaacc
     5401 cctggctgaa caggccaggc gcatcctggt ggaattaggt tacgacaata ttcaattccg
     5461 tattgataac ggcttctatg gttggcctga aaaggcgccg ttcgatgcgg taatcgtcac
     5521 cgcggccgca acggagattc ccgggtctct ctttgcccag ctgaaaccgg gcggtagaat
     5581 ggtgctcccg gtgggattcc cgggagagat tcaaaccctg tggcgaatca caaaaagtat
     5641 ttatggggcg aagatggaga ggtttgatcc ggtccgtttc gtcccccttc aaaatccgga
     5701 accgtcatcc acccagccgg tggaataa
//
LOCUS       DASVMB010000515         4793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_209566,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000515 DASVMB010000000
VERSION     DASVMB010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209566"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VF531_14525"
     CDS             <1..405
                     /locus_tag="VF531_14525"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX7715228.1"
                     /translation="HTLASAYVPLGRDATVVYGGIDFIFQNNYQNPILITAKIQSPYL
                     TIAILGRKTGWERISLENRVIETYPYQIKEIPDPDLRRGVRKKQQTGQKGYKVELWRE
                     IYLESGAVRKELVNTSIYPAQPEEYKVGTKKS"
     gene            526..1485
                     /locus_tag="VF531_14530"
     CDS             526..1485
                     /locus_tag="VF531_14530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7715229.1"
                     /translation="MRQNFPLARIVVLASPIAARVLEGNPFHLEVKVVSDQWELFKSL
                     AEVRKKGFDLAVGLSQMGTLFTRYCAAKAMTDFNLTRYRSDLSVVQLCLDVIQAAGLK
                     TAAPRTEFWFRESVRDNASARIDSLLTDFKCDPAQPLVALHCGGHYFIRKRWPLPHFG
                     QLIRILRSRIGVQIVLVGGREDREIITQFGEELSGALDLVGYLKLEETAVLLQRSRLF
                     IGNDSGPLHLAAALQVPTIGLFGPTNPQQFYPYQSPRHTYIYKAVSCSPCFKFRGSIW
                     QHLPRCTRSYCMEAIAPEEVAREAVRRLKEPGEAEPCFNAGKP"
     gene            1469..2500
                     /locus_tag="VF531_14535"
     CDS             1469..2500
                     /locus_tag="VF531_14535"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7715230.1"
                     /translation="MQENRKIHTGIWLAVLFSVSSIYFIFHSFKGIHLSAGINRLNWF
                     YVVFGFFVQFLVWLAKACRMYAIADGMGSHVPLTRFFQIYMATCFVSHVTPFNSGGTP
                     LEVYLLTKQGIAVGTASAITVVDLGLNTAMFFILAVMAAASNFGEFTRMLDGGRAGTR
                     WLWMIGIVMVVMIILAILVGYYRRPKSRGPGWTKLRQFLHKKGWLDNLAREARLFKEG
                     WLMLSRGNPSRIFWASLATVVYWLLYLLLAPLIIWALGKPVSFWGLIGAQLIFNLAQI
                     FIPTPGGSGGSELILGYFFRSYTGVSLIGLFVLIWKVYTFYSTLILGGIFFLRLTRGG
                     SVPKSAKND"
     gene            2686..3066
                     /locus_tag="VF531_14540"
     CDS             2686..3066
                     /locus_tag="VF531_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7715231.1"
                     /translation="MLLEVGQIVEGKVTGITNFGAFVELSNGQTGLVHISEVADEYVK
                     DVNHFLKQNDLVKVKVLNLDKGKIGLSIRQAQPRSERGRQNTKSFEDKLAKFMKDSDE
                     RQSDLRRNTDAKRGGRGGGRAPSM"
     gene            3226..3828
                     /locus_tag="VF531_14545"
     CDS             3226..3828
                     /locus_tag="VF531_14545"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX7715232.1"
                     /translation="MDKDSRRKLIEAAIPLFAEKGYAAVSIRELAAAAQVNSALITYH
                     FGGKEGLYRAVLEDQFTQIVELFSKKTFSDLSPAEKIRESAQDMIEFQSRNPFFRKLL
                     FGELSHTTPSFEGILRDYSFRLYRLFHETLETGIAQGEFRQDLNSSYALFAILGMVSY
                     YFIAHSVIEKAVPELRLDDHIFEEQAVEMIFCGINENKTK"
     gene            4031..>4793
                     /locus_tag="VF531_14550"
     CDS             4031..>4793
                     /locus_tag="VF531_14550"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7715233.1"
                     /translation="MAKKGVRKIGVFGIIAMLLVFGSFCNAELSGDDILKKVDGIMDS
                     PATKIEAVMSTIGNGAEKNSQMLLYTKKNAQGENRTLIQYLYPPVDKGTKFLSLGSVD
                     KMWMYLPKVEKLVRIAGSMVKQSMMNSSFSYEDLLDRGNLAKDYSAQVVGNSMIDDSN
                     CYVLMLNAKKGSAHYRQIKLYVHPDYFIPVKEEFLSGNGAVTKMSYQTDIAQMGGRTI
                     PTKITFQDLKEKDKQTTLTIKSVQFNADIPERYFTQ"
BASE COUNT         1273 a          984 c         1203 g         1333 t
ORIGIN      
        1 cacactttag ccagcgctta tgtgccttta ggccgtgatg ccactgtggt ttacggagga
       61 attgacttta tttttcaaaa taattatcag aatccgattc ttattacagc caaaatacag
      121 tcaccctatc tcaccatcgc cattctggga cgaaaaaccg gttgggaacg gatcagtctg
      181 gagaatcgcg taatcgaaac ctacccatat caaatcaagg agattcctga tccggatttg
      241 cgaaggggag tccgtaagaa acaacaaaca ggacagaaag ggtataaggt tgagctgtgg
      301 cgggagattt atctggagag tggcgcggtc cggaaggaat tagtaaatac cagtatatat
      361 ccggcgcaac ctgaagaata taaagttggt acaaaaaaat cgtaacccta tttttcaaga
      421 attaaagggg cggttggcac ggttcagcca gatactccaa agatcgtcct gctctgttta
      481 agccctctgg gtgatacatt atttgccact ccggctattc gagctgtgcg tcagaacttt
      541 ccgctagctc gaattgttgt tttagccagt cctatcgctg cccgggtgct tgaggggaat
      601 ccctttcacc tggaggtgaa ggttgtcagc gatcaatggg aactcttcaa atcactggcg
      661 gaggtccgga aaaaagggtt tgacctggct gtcggcctgt cccaaatggg aaccttgttt
      721 acacggtact gtgcagcgaa ggctatgacc gattttaact taacccgtta ccgtagtgat
      781 ctttctgtgg tccagctctg tttggatgtt attcaagccg ccgggctcaa gaccgccgct
      841 ccccgcaccg agttttggtt tcgggaatcc gtaagagaca atgcatccgc gcgcattgat
      901 tcactgctta ccgattttaa atgtgatccg gcgcaaccac tggtggcgct acactgcggg
      961 ggacactatt ttattcgaaa acgttggccc ttaccccatt ttggtcaact catccggatc
     1021 ctccggagcc gaatcggggt tcagatcgtt ttagtgggag gccgggaaga tcgggagatc
     1081 attactcaat ttggggagga actttccggg gctctggatc tggtcggata tttaaagctt
     1141 gaagaaacag cggttttatt acaaaggtcc cggttattca tagggaatga ttcggggccg
     1201 ctacatctgg cggccgcgct ccaagtgccc accatcggat tgttcggacc gaccaacccc
     1261 caacagttct atccttatca atcaccaagg catacctata tttataaagc cgtctcctgt
     1321 agtccctgct ttaaattcag gggcagcatc tggcagcatt tgccccgttg cacccgttcg
     1381 tattgtatgg aggcaatcgc cccggaagag gttgcccggg aggcggttcg gagattaaag
     1441 gaaccggggg aggccgaacc ctgtttcaat gcaggaaaac cgtaaaattc acaccgggat
     1501 ctggctggcg gtactcttca gcgtcagtag catctatttt atctttcaca gttttaaagg
     1561 gattcatctt agcgccggga taaacaggtt aaactggttt tatgtcgttt ttggtttctt
     1621 cgtccaattt ttagtctggt tggccaaggc ttgccgaatg tacgccattg cggacggtat
     1681 gggctcccat gtacctttga cgcgcttttt ccaaatctat atggcgacct gctttgtctc
     1741 tcatgtgact ccgtttaact ctggcggtac cccgcttgag gtttatctcc taacaaaaca
     1801 gggaatcgcc gtaggaacgg cttccgccat caccgtggtc gatttgggat tgaatactgc
     1861 gatgtttttc attttggccg tgatggccgc cgcttcaaat ttcggagagt ttaccaggat
     1921 gcttgatgga gggcgggctg ggacccgttg gttatggatg attggaatcg tcatggtagt
     1981 gatgatcatt ttggccattt tggttgggta ctaccggagg cctaaatctc gcgggcctgg
     2041 ttggactaaa ttacggcagt ttcttcacaa aaaagggtgg ctggataacc tcgcccggga
     2101 ggcgaggctg tttaaagaag gttggctgat gctctccagg gggaacccca gccgtatttt
     2161 ttgggcatct ctggcaactg tggtatattg gttgctttac ttgcttttag cgccgctgat
     2221 catttgggct ttgggaaagc cggtatcatt ttggggacta atcggtgcgc aacttatttt
     2281 taatttagca cagatattca ttccgactcc gggagggagc ggcggttcgg agttgatttt
     2341 aggttacttt tttcggagtt ataccggggt aagtttaatc gggttgtttg tcctgatttg
     2401 gaaggtgtat acgttttaca gcaccctgat attgggagga attttctttt tacggcttac
     2461 ccggggcggt tccgtaccca aatcggctaa aaatgattaa attaacacgc ctttccattg
     2521 atgttgagaa attggccgac cggcttgtct tgacaacact tttcagtgta cgttataata
     2581 tattagaata gatggcgtaa cgctgctgac gtttcgtgat ttatcgagtt tacatcgagt
     2641 agaacgtccc cgctgcgcaa atgaaagagg aggaatcatt ggaggatgtt gttagaggtt
     2701 ggccagatcg ttgagggaaa agtcacggga atcacaaact tcggagcttt cgtggagctt
     2761 tcaaacggac aaaccggttt ggttcacatt tcagaggttg cggatgaata cgtaaaggat
     2821 gtcaatcatt ttctgaaaca aaatgatctg gtcaaggtaa aagttttaaa cctcgacaaa
     2881 ggcaagattg gtctatcgat ccggcaggct caaccgcgtt ctgagcgggg ccggcaaaat
     2941 acaaaatcct tcgaggataa actggctaag tttatgaagg attcagatga acgccaatcg
     3001 gatctgcgcc gcaatacgga cgccaagcga ggtggccgcg gcggcgggcg tgctccgagc
     3061 atgtaagccg aagtccagta ttaatcggtt taattttata aaaataatcc ggcgattacg
     3121 ccggattttt tatttttgca attttactac gtcaaagaaa aaggtagaca aaaaataatt
     3181 tcgtgatata atctaatcaa tcgattaatt atagggagta agaaattgga taaagattcg
     3241 cgccgtaaac taattgaagc tgccatcccc ctttttgccg aaaaaggtta tgctgcggtt
     3301 tccatccggg agttggcggc ggcggctcaa gtcaacagtg ccctgattac ttatcatttt
     3361 ggaggaaaag aaggattata ccgggctgtg ctggaggatc aatttactca gatcgttgag
     3421 ctattttcga aaaagacatt cagtgatcta tcacccgctg aaaaaatccg ggagtctgct
     3481 caggatatga tcgaatttca aagtagaaac cccttcttcc gtaagctttt atttggggaa
     3541 ttaagtcata ccacacccag ttttgaaggt atattaaggg attactcttt tcggttatac
     3601 cggcttttcc atgaaacatt ggaaacaggt attgcccagg gcgaatttcg gcaagatttg
     3661 aattcttcct atgcgctttt tgccatcttg ggtatggtta gttattactt tattgcccat
     3721 tctgtaattg aaaaggcagt tcccgaactg agactggacg atcacatctt tgaagaacag
     3781 gcagtggaga tgattttttg tggaattaat gaaaataaga cgaaatgaaa gatttttttt
     3841 ggttttatgt taatcaaacg attaattaaa tttgatgaat taatcattcg cttgattaaa
     3901 attccccagg cagaagttat atcgcttgta tggcagatga accgatcagg tccgaagaaa
     3961 gtaggtgatg cgcaaaaata cccaaggtgt tttgcacaat caattaatga aaacgggggg
     4021 gtttatcaaa atggcaaaaa agggtgtaag aaagattggc gtctttggga tcatagccat
     4081 gcttttagtt ttcgggagtt tttgcaatgc cgaacttagc ggggatgata tcttaaaaaa
     4141 agtagatggt atcatggatt ccccggcaac taaaattgaa gccgtaatga gcacgattgg
     4201 caatggtgcc gaaaagaaca gtcaaatgtt gctttacacg aaaaagaatg ctcaaggaga
     4261 aaatcggacc ttgattcagt acctttatcc accggtcgac aaaggtacga aatttctctc
     4321 attgggaagc gttgacaaaa tgtggatgta tcttccgaag gtcgagaaac tggtgcgaat
     4381 cgcaggaagc atggtcaaac agtcgatgat gaactccagt ttctcatacg aggatctatt
     4441 ggatcggggt aatttggcaa aggattattc cgctcaagtc gtaggaaatt caatgatcga
     4501 tgattcaaat tgctatgtct tgatgttaaa cgccaaaaaa ggcagcgccc attaccgcca
     4561 gatcaagctt tatgtacatc cggactattt tataccggta aaagaagagt tcctctcggg
     4621 taacggagct gtgaccaaaa tgtcttatca gacggatatt gcccaaatgg gcggcagaac
     4681 gattcctaca aagataactt tccaggattt aaaagagaaa gataagcaaa ctacgctaac
     4741 aattaagagt gtgcaattta atgcggatat cccggagcgt tactttaccc agg
//
LOCUS       DASVMB010000516         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_62390,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000516 DASVMB010000000
VERSION     DASVMB010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62390"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VF531_14555"
     CDS             complement(<1..1536)
                     /locus_tag="VF531_14555"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715234.1"
                     /translation="MRQVIAAMAQHPDFKAIKEHFDRHHQDLLTGVDGSQKTAAIAAL
                     TQELDHCLVLSYNNHQAYRMALDLEEFCGPKRVIFFPSNELLPHEEAFEPEVTAQRIE
                     AMGRILQGERLLIVTSWEALQRRLISPAKFSEFTLPLEIGMEYKLSDLLSRLVKMGYE
                     RVDQVQAIGQFSQRGDLVDIFPLDREDAVRIEFFGDEIDSLRSFKVEDQLSKESLQKL
                     IILPAREGLWHQAEFNNSLDRIQEDLNSQVSKLENNGKVNEAEALRNRFGEALERLSQ
                     GHQLPGADQFLPWVDSQLVTLFDYLPDCRIILDEPVRGLDVYRGLEEETAGIISNFLE
                     KGVILPREQEMLVSLAELEARIWPHQSGHHPWSLSLLAKTPKGTPPLHTVTLPFKTAP
                     LFHSKLPLLAEELERRQREGATTILAVSNKEKARRLKEILREQGISSRLPQESDDAAQ
                     GTARGAFVQIQTADLESGFEWAPGRLLVLTEAEIYGRQKKRYPARFQQEGSKINSFTD
                     LKVG"
     gene            complement(2389..2991)
                     /gene="pth"
                     /locus_tag="VF531_14560"
     CDS             complement(2389..2991)
                     /gene="pth"
                     /locus_tag="VF531_14560"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX7715235.1"
                     /translation="MYCLVGLGNPGTEYQQTRHNAGFLVIDQLAQDNRIDLGRRQFKS
                     MYGKGRLGDREVLLVKPETFMNLSGQAVSSWVNYFKIPLNQVLIIHDDLDLPLGTVRL
                     KPSGSSGGHRGLSSIIELLGTSEIPRLRLGIGRPARGTTVVDYVLAGFSGAESKLFQQ
                     STTRAAAAACSFVTEGLEYAMSRFNGKAGDSSVSDDSISD"
     gene            complement(3086..>3337)
                     /locus_tag="VF531_14565"
     CDS             complement(3086..>3337)
                     /locus_tag="VF531_14565"
                     /inference="COORDINATES: protein motif:HMM:NF026044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX7715236.1"
                     /translation="CLPANIPDRITVDVSKLTMGSGIHVKDLNVAEGIKTLNGLEEAV
                     VLAVAPTVAVEEKPAAAATEAAAPEGAADAKDATKKEEA"
BASE COUNT          895 a          916 c          731 g          795 t
ORIGIN      
        1 cccaaccttc agatcggtga aggaattaat cttcgatcct tcctgttgaa aacgggcggg
       61 gtaacgtttt ttttgcctgc cgtaaatctc ggcctcggtt agcaccagta accggccggg
      121 agcccattcg aaacccgatt ccaaatcagc ggtttgaatc tgcacaaagg cgccacgcgc
      181 ggttccctga gcggcgtcat ccgattcctg aggcaatcgg gaagagattc cctgctcacg
      241 gaggatctct ttcaggcgcc gtgccttttc cttattcgac acggccaaaa tggtagtcgc
      301 gccttccctc tgtctgcgct caagttcttc ggctaataat gggagtttgc tatgaaaaag
      361 cggcgcagtc ttgaaaggta aggtcacggt atgaagcgga ggagttcctt tgggcgtttt
      421 ggcaagcaga gaaagagacc agggatgatg tcccgattga tgcggccaga tccgggcctc
      481 cagctctgcc aggctcacca acatctcctg ttcgcgcggg aggattactc ctttttccag
      541 gaaattactg ataataccgg ccgtttcttc ttccaaacca cgatagacat ctagcccccg
      601 gacaggctca tccagaataa ttcggcaatc cggcagataa tcaaagagtg tcacaagttg
      661 tgaatcgacc cacggtaaaa attgatcggc tccgggcaac tgatggcctt gggacaaacg
      721 ttccagcgcc tcgccaaatc ggtttcgtaa ggcttcggcc tcattcactt ttccgttgtt
      781 ttcgagtttc gatacctggc tgtttagatc ctcctggatc cggtccaggg aattattaaa
      841 ctcagcctga tgccataggc cttcccgggc cggcagaata atcagtttct gtagagactc
      901 cttggagagc tgatcctcta ccttaaatga gcgaagtgaa tcgatctcat cgccgaaaaa
      961 ttcgattctt accgcgtctt cccggtcaag cggaaaaata tcgaccagat cgcctctctg
     1021 gctgaactga ccaatagctt gcacttgatc gactctttca taacccattt taaccaacct
     1081 ggagagtaaa tcggagagtt tatactccat tccgatctca aggggcaagg taaattcgga
     1141 aaacttagcc ggagaaatca agcggcgttg caaggcctcc caggaagtta caatcagtaa
     1201 ccgttctcct tgcaatattc ttcccatagc ctcgatacgc tgcgcggtaa cttccggttc
     1261 aaacgcttct tcatggggga gtagttcatt actgggaaag aaaatgaccc gtttaggacc
     1321 gcaaaactcc tccaaatcga gcgccatccg gtacgcttga tggttattat aactcaatac
     1381 caggcagtga tcgagttcct gcgtaagtgc ggcgatggcc gcggtttttt gacttccgtc
     1441 aaccccggtc aataaatcct gatgatgacg atcaaaatgt tccttgatag ccttaaaatc
     1501 ggggtgttgc gccattgcgg cgataacttg cctcatgctg tcctccagat atcttcgcgc
     1561 gctcgggcgc gcaacccagc tttgtccaaa tttcctcaaa caaacaccaa caagggctca
     1621 atggttgagc ccgggagatc ctgctaacac ataagcgcac tcaccgaatc attatataag
     1681 atatgaacgg cttccccaac aattgatcga aaagcaatgg aaagccatga ttatacccta
     1741 gtcccaaatt aacccgaggt ggagccattc tttgattgcc ggagaagcaa ccaattgttc
     1801 cagagcatat gagtaacaag ggccaataaa aaattaagcc aaaccggatt actttggcaa
     1861 agaaagaaca gccccgttac gagatgcatt acaactccaa ccccgattat aggaatatcg
     1921 atgcgccgtt ttacatgaaa cgtatcaaaa aggccttcac ccaaaccaaa taaagcatat
     1981 aacaaccatg gggtcagacc gaaccaaaag cacgccccaa atttcaacaa ttcttcacca
     2041 attggaaccc caaaacccgt tcgaaatcgg aaattacggg aaaacgactt taaaagtata
     2101 tcccacaagt acgccaatcc aaccgttatc cagcccacca gcagatactt tacggcggct
     2161 gtaaccggag cgatcatcat tgataaatag tgtaacatcc aaccaaaaat gtgaatatcc
     2221 gcatattatt tcagccagac ataactaact tcaggccgtc tgtatccgaa gcaaagaatc
     2281 caccaaaatc caaaaccatt ttggatgaac ctatagcccg gaaataggtt tacccctatc
     2341 gggcggtttt attcgaaggg agtatccaac cgattgaatc cgcaactgtc aatcggaaat
     2401 agaatcatcc gaaacggatg aatctcctgc cttcccgtta aaacgactca tggcgtattc
     2461 caagccttca gtcacaaagg aacaggcggc cgcagccgcc cgggtcgtgc tttgttgaaa
     2521 caacttgctc tcggccccgg aaaaaccggc gagtacatag tctaccacag tcgttcctct
     2581 cgcgggtcgg ccaatcccca atcgtaggcg gggaatctcc gaagtaccca aaagctcgat
     2641 gatggaactt aaaccgcgat gccctccgga actgccgctt ggtttcaacc gtaccgttcc
     2701 taacggcaga tcgagatcat catgaatgat gagtacctga ttgagaggaa ttttaaaata
     2761 gttcacccag gaagaaaccg cttgaccact caagttcata aaggtctccg gcttgaccaa
     2821 aagcacctcg cggtcgccca gtcgcccttt accatacatg cttttaaact gacggcgccc
     2881 cagatcgatc cgattgtcct gcgccagctg gtcaatgact aaaaacccgg cgttatgccg
     2941 ggtttgttga tattccgttc cggggtttcc taatccaacc aagcaataca taacataaac
     3001 acccccggca gggctaactt cagccccggg tttaaaactg gctcttaaac caaaccatat
     3061 atatctccaa ccggaagaat attttttaag cctcttcctt cttggttgcg tccttggcat
     3121 cggctgcccc ttcaggagct gctgcttcag ttgccgctgc tgccggcttc tcttccactg
     3181 ccacagtcgg agctaccgct agaaccaccg cttcttccag gccattcaac gtttttatgc
     3241 cttccgcaac gttcaaatcc ttgacatgaa ttccggagcc cattgtcaac ttgctgacat
     3301 ccaccgtgat ccggtccgga atattggccg gtagaca
//
LOCUS       DASVMB010000517         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_432804,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000517 DASVMB010000000
VERSION     DASVMB010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432804"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF531_14570"
     CDS             complement(<1..741)
                     /locus_tag="VF531_14570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7715237.1"
                     /translation="MIEKIQHEREAVAERERNQMRFLQIAAHELRNPMASIKGILALI
                     NRRLSGGKPIESWNIISMVQIMEQEIDRLAHLLDEIMDAYRMQGGRFFIKRQRMDLFE
                     VLRAALQPFLASQDNPLFLFEPVTTGPVWLYGSFTRLDEVFRNLFSNAVKYSLGRGTI
                     MVRLEIKNGKAVVSIKDQGLGIPENQLGKVFEGFYRASNLRRDSDPGGLGLGLYICRD
                     IVQRHGGRIWAESEEGAGATFFVELPLCP"
     gene            complement(1240..1629)
                     /locus_tag="VF531_14575"
     CDS             complement(1240..1629)
                     /locus_tag="VF531_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715238.1"
                     /translation="MARILVIDDEPTILLVLQEILEDEGYMVFTAANGLEGMKVLGEG
                     PPPDVILIDLLMPGMGGREFIRLLRTNDNQSRIPVILITGAIPNTEDFPVEGSYQDVI
                     SKPFDILDIIHKVNRLLKVMNPLPAKD"
BASE COUNT          580 a          545 c          382 g          578 t
ORIGIN      
        1 cgggcataaa ggcaactcca caaagaaagt agcaccggca ccttcttcac tttcggccca
       61 gatcctccct ccatgacgct gtacaatatc tcggcagata taaagaccta accccaaccc
      121 acctggatcg gagtcccgcc gcaggttact ggcacggtaa aaaccctcga atactttacc
      181 caactgattc tcgggaatcc ccaaaccttg atctttaatg gatacgacag ccttcccgtt
      241 tttaatctcc aaacgaacca tgatggtacc ccggcccaac gaatacttaa cagcgttgct
      301 gaacaagtta cggaaaactt catctagccg agtaaagcta ccatacagcc agactgggcc
      361 ggtcgtaacc ggttcaaaaa ggaatagagg gttatcctgc gaggctaaaa aaggttgtaa
      421 agctgcacga agtacttcga acaaatccat ccgctgtctt tttataaaga accttcctcc
      481 ttgcatgcgg tacgcatcca tgatctcatc cagcaggtgg gccaggcggt ctatctcctg
      541 ttccatgatc tgcaccatac taatgatatt ccagctttcg atgggtttcc ccccgctcag
      601 tctgcggtta atcaatgcca aaataccctt gatgctggcc atcggattcc gcaattcatg
      661 agctgcgatc tgcaaaaatc gcatctggtt tcgttcccgc tctgccactg cctcccgttc
      721 atgttggatt ttttcaatca ttcgacgccc ttgaagatca cggcatattg ctaagtaatt
      781 tccattgggt aacttggtta tattcatctc taccgaatga atcaatccat gacggtccct
      841 tataaagatc tccccaagaa acatcccttt gcgttgcaac agacttattt ttcttgaggt
      901 aatcttagct ttatccggag gtattaaatt ctccacatta agcttgcata attcatcttt
      961 ggtgtaatcc aacaaccggc atgcggctga attcgctgta accagcgata atcccggcgt
     1021 tacgactaga atcgcatccg atgtgtactc aaaaatatca cggtaaagtt cggggttatc
     1081 acttggcgta ttttgatcat acttttcaaa aatggttgga gccccctccc acctaagttt
     1141 gcctaagcaa gtatctcaat atgccacgcc aaattgctac ccattttgaa tatctatcaa
     1201 cgaacaatca atatttttca ggctgcttgg agtccaccat caatcctttg ctggtaaagg
     1261 attcataacc ttcagcaatc ggttgacctt gtgaataata tcaagaatat caaagggctt
     1321 gctaattaca tcctgataac tgccttccac aggaaaatct tctgtgttgg gtatagctcc
     1381 ggtaattaga attacaggta tccgtgactg attgtcattg gtacgaagca gcctaatgaa
     1441 ctcccggcca cccatacccg gcatgagtaa atcaattaaa atcacatcgg gcggtggtcc
     1501 ttccccaaga actttcatgc cttccaatcc gttggcggca gtaaacacca tataaccttc
     1561 atcttccagg atttcctgca acaccaagag aattgtcggt tcatcatcga tcaccaaaat
     1621 ccgcgccatc ccgataacct cctttggttt caaaacagga aacaaacatt cggaaacggt
     1681 gattctcttt tataaacgtt gcataaatac ccttagaggt attttctgaa acatttgaat
     1741 tttgcaaacc atgaaattat ttatttctac aaccttcaaa tattacctgc taagtggagc
     1801 ctgattttat catcatgtct tccagaaatc caaattttta ggataaatgg aaacaccctt
     1861 tgataagctg cccgatgcta gtagagacat tcggtaactc caccttcatc accataagga
     1921 tgctgatcgc caaccccgta cctaatattc gctaactctt tgtaggcatt tatttggact
     1981 gtgctgtaat aaaagagaat cccgcttctt tcgaagcggg attctcaaaa atttactcct
     2041 ggcgatgacc tactctcccg gccggtcgcc cgacaagtac catca
//
LOCUS       DASVMB010000518         7035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_503442,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000518 DASVMB010000000
VERSION     DASVMB010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503442"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF531_14580"
     CDS             complement(<1..207)
                     /locus_tag="VF531_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX7715239.1"
                     /translation="MKVVFPDGAFREYPDGTSPLQVAESIGTRLAKEVIAAEANGKLV
                     DLKLPLEGEVKLHLIKPTDPEGLEI"
     gene            complement(766..1338)
                     /locus_tag="VF531_14585"
     CDS             complement(766..1338)
                     /locus_tag="VF531_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX7715240.1"
                     /translation="MSQSILASDKEQINLTATAVVLEVPFPVSQRIQELRERFDPVLA
                     RSVPVEVTIAGSSGLAVLAPGQDSAAVFATLQQIAAATPSFTAAFGRAFRFPHSTVYA
                     LTVKPLRPFRELHQRLKTAPVRFLPSPFHFMPHCSLHIWGELPETLAQEIIQTRIPEH
                     FGISNLALYQRVSDTAVALVARFPLTGMGD"
     gene            complement(1353..3023)
                     /locus_tag="VF531_14590"
     CDS             complement(1353..3023)
                     /locus_tag="VF531_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX7715241.1"
                     /translation="MRWEEFSRIDLEALVPPRIMERGLNYYREGQLIKACRIGPVLCG
                     VLLGSGANYHARLWWTDLGLKWDCNCPYPEFCKHLVALALAWIERGSGFIDLTLQLDQ
                     ICGNPQAMPEFVRGLAFRNPLDFGELWDEFVRGRCDAGTEIAVASAAPSERGLLNLVR
                     NLFPSHPLTPETIRELWDRVERISRLLLDRMVQDDIGAARALAELTRRMTELFPVYPD
                     PVFRSFLTELGDMVYLKLAKAGPEIRAVFLEVWWALYFNPELWDIRPELGKWLRQFRG
                     SDPQLFSAAIRETLTGQSQFLILIGLFELLDEPTPAGNPGSPDTSSFRQELNDIRCKL
                     RENDEGCLWLIDRLREVDPDQALRIANTGLRRNSGLKDSYRERLIILHQLRGDLRQAA
                     ALSFVQFREKPVLEEYLRLRDLLAKSPSDWAIYRGRIKELMENQALGRLAIQIALADR
                     DSTALVEQWPDILADPQLLLNTAGLFQEEDFPLELAEFFPQLIRALLETGGPEFYKSA
                     RATLIRFKKFCYRHDHRSDWDDLRRSLLEEYRDDLGFRYKFGSLLDEL"
     gene            complement(3400..4407)
                     /gene="tsaD"
                     /locus_tag="VF531_14595"
     CDS             complement(3400..4407)
                     /gene="tsaD"
                     /locus_tag="VF531_14595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7715242.1"
                     /translation="MAEFLTLGVESSCDDTSVAVVADGFKIRSNIISSQIDLHAKFGG
                     VVPELAARCHLEAVLPIYQMALAEAGVKLEQIRLVAATYGPGLVGSLLVGLSFAKGLA
                     IAAGIPFIGVHHIEGHIYANVLERPELKPPLICLTVSGGHTDLLFIEKWGSYRILGST
                     RDDAAGEAFDKIARALGLGYPGGPYIDRIARGVPADLEFAQLRGLANSYDFSFSGLKT
                     AVLNYLHHQKQTGREIDIPRVAASFQRIAVEQLTGKLFLAVRDYGVDTILLSGGVAAN
                     SYLRSRCEQEADRLGKKVYYPEPRLCTDNAAMIAAAGYFHYSHGDRTGIEVQAQPDLV
                     L"
     gene            complement(4580..6298)
                     /locus_tag="VF531_14600"
     CDS             complement(4580..6298)
                     /locus_tag="VF531_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7715243.1"
                     /translation="MIKYLQKFFPKVEAYHGVGILSNLNFKTKIFLAFGVLLLGLILQ
                     GFWAFSRYFQIAAEGRYLNQMKQELNTLKTIKEGSPDLLTRCTVAVKTGQPVELGNSL
                     ARIETGIARIVKSDNMNTDSLHNRFQALREQLKYYNGANLDSAQYGSVKRLYSQYRNA
                     LIQTETALNRVYSNRNRGMLLRMAGFTLLVLVILVLMVVVSGWLVIAAVRTITEPAVL
                     ITEQLNQDRLAGAANHIPAYSPDGLGVAALLLNEGFRSWDEIFEKLKNSTAKLENLCQ
                     ELVGQVKLREISEIQFNEVYKSIDTYINEQNETLQKSNEHLTFIISNITELQRIPEQL
                     GQFAEELDGVVIQAEGQMEELLNRQVTIENCTPLILDLFHNLAEMSTKVDSVMGVLNG
                     VSEQTELLAFNTAIEAARAGNKGLGFGVVSQEITKLVERSKKATEDLHAAVNAIQARQ
                     ESLREELPQAGVNIELLGELRRDAGEISNRVFTAAHSEVDNMLQLVQVLEEIIAKSET
                     IIQEANVIAGVPATEKDELQNIDLEILDYQLNLKEAVRISEKTSESSGNLRRALALAT
                     EEQHSA"
     gene            complement(6346..>7035)
                     /locus_tag="VF531_14605"
     CDS             complement(6346..>7035)
                     /locus_tag="VF531_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7715244.1"
                     /translation="LAQAGEVIKVGSSEVNRLNSKLDLRANNSAQIVASLKQLRVMMD
                     QLQQIVTVFEGLTEQTSVLSLNAAIEAARAGTAGDGFDIAATEIGELADRIGKIPHEL
                     LLLLNKEKKQTEQAIHTYEGLLPQNNQVKQYLAAFETQLAAFWEKFSVMLEQIQEFES
                     LVHQYEVRRLSLEELTGFLAELNRRIPEDYSKAATALDAVDKPDHFPLSMSELENLVI
                     EVNQLLAKMEN"
BASE COUNT         1802 a         1940 c         1610 g         1683 t
ORIGIN      
        1 aatctccaat ccctccggat cggtgggctt gatcaaatgg agtttgacct ctccctctaa
       61 gggtagtttc aaatcaacca acttgccgtt agcctcggcc gcaatgacct ccttggctaa
      121 ccgcgttcca atggactccg ccacctgaag cggactggtt ccatcagggt attcccggaa
      181 agcgccatcg ggaaaaacca ccttcattct tatcactcct tttaagaatt tcgcctaaaa
      241 agaattttcg tgtatttggt gattttcgtg gttaaaaaag tttctatcgg tgtgccgatt
      301 ttatccacaa cgaaacacac caaagacacg aaaaagggta tgtcattatt tccgctgttg
      361 aagtaattta tcataaagca gttctgaata aaaataaaac cccatcccta acagtatagg
      421 gacggggaat ccgtggttcc accctgattg gtcaaaaccc attgtttcga ggctcgaccc
      481 gcttaatacg gctttatcgc ggccgtgcgc cgacttatgc agtatcggta taaataatcc
      541 gatcagcctt cgccggaacg tccgaggtgg ttatggaaac tataaaagac gcaaatctca
      601 gtctgttaca gctttaccgt taccttgccg gaaagctctc agccttgctc tcctctctgt
      661 aagccggact aacggttcga ccctcttcat ccgtttgaat cgtataaatt gaaattagta
      721 tataacttca cagctatttc gtcaagccaa gattaacgga tttcgttagt cacccatccc
      781 ggttagcggg aaccgggcca ccaatgccac agctgtatca gagaccctct gatacagtgc
      841 caggttgcta atgccaaaat gctccggaat ccgggtctgt ataatctctt gtgccaaggt
      901 ctccgggagc tcaccccaga tatggagcga acagtggggc atgaaatgaa agggtgaggg
      961 caagaacctt accggggctg ttttcaaccg ttgatgcaac tcccgaaacg gccgcaacgg
     1021 tttgacggtt aaggcataaa cggtggaatg ggggaaacgg aatgcccggc caaaagcagc
     1081 tgtaaatgaa ggcgtggctg ctgcgatctg ttgcaacgtc gcaaatacag ccgcactgtc
     1141 ttgtccggga gcaagcacag ccaatccgga ggaacctgcg atggttacct caaccggtac
     1201 actccgcgcc aaaaccgggt caaagcgctc ccggagttct tgaatgcgct gactgaccgg
     1261 aaagggaact tccagcacta cggcggtagc ggtaagattg atttgttcct tatcagaagc
     1321 gagtatggat tggctcattg tgaaaccccc acctataatt cgtccaaaag tgaaccgaat
     1381 ttgtatcgaa atcccaaatc atcccggtac tcctcgagta aactccggcg aagatcgtcc
     1441 cagtcactcc ggtgatcgtg acggtaacaa aacttcttga acctaattaa tgtagcccgg
     1501 gccgacttgt aaaattccgg ccccccggtc tcaagtagcg cgcgaattaa ttgggggaaa
     1561 aactccgcca gttccagagg aaaatcctcc tcctgaaaca acccggcagt attcagtagt
     1621 aattgcgggt ctgccaggat atccggccac tgctcaacca aggctgtact atcccggtct
     1681 gcgagagcga tctgaatcgc cagtcgcccc agggcttgat tttccatgag ttctttgatc
     1741 cgcccccggt agatcgccca gtcactgggg gatttcgcca ataaatcccg gagtctgaga
     1801 tactcttcga gaacaggctt ttcccggaat tggacgaaac taagtgcggc agcttgacgg
     1861 agatcacccc gcaattgatg caggatgatc aaccgttccc ggtaggaatc tttcaaaccg
     1921 ctattccgcc ttaaccctgt attggctata cgcaacgcct gatccggatc aacctcccgc
     1981 aaccggtcga taagccataa gcaaccttca tcgttttccc gaagtttaca acggatatcg
     2041 ttcaactcct ggcggaagct cgacgtatcc ggagaccccg gattacccgc cggagttggt
     2101 tcatccagga gttcaaaaag tccaatcaat attagaaatt ggctttggcc cgtgagagtt
     2161 tcccggattg cggcactgaa aagctgtgga tcagaaccac ggaattgccg caaccatttt
     2221 cccaattccg gccggatatc ccatagctca ggattaaaat aaagcgccca ccaaacctcc
     2281 aaaaaaaccg cccggatctc gggcccggcc tttgccaatt tcaaataaac catatctccc
     2341 agctccgtca ggaagctccg gaatacggga tcgggataaa ccgggaataa ctccgtcatt
     2401 ctgcgggtga gttcggccaa tgcacgggca gccccgatat catcctggac catacggtca
     2461 agcagaagtc gactgattcg ctctacccga tcccacagtt cccgaatggt ctcaggagtc
     2521 aacggatggc tggggaaaag gttccggacc aagtttaaaa gaccccgctc acttggagcc
     2581 gccgacgcga ccgcgatctc cgttccggcg tcgcaacgcc cccgaacaaa ttcatcccag
     2641 agttctccga aatccaaagg attccgaaac gccaaccccc ggacgaattc cggcattgct
     2701 tgggggttac cacaaatctg gtccaattgc aaggtaagat cgatgaaacc ggaaccccgc
     2761 tcgatccagg ccagggctag tgctaccaga tgcttacaaa actcgggata ggggcagtta
     2821 caatcccatt taagccctaa atcggtccac cataaacggg cgtggtaatt ggcgccactg
     2881 cccaacaaca ctccgcagag gaccggaccg atgcggcagg ccttaatcaa ttgtccctcc
     2941 cgatagtagt ttaagccccg ttccatgatt ctgggtggta ccaatgcttc caggtcaatc
     3001 cgggaaaact cttcccatcg catggctaac cctcctgaga tcaaaatgga ggtgattttg
     3061 ggacaatctt gaaattttac cgttttggct tcaatttata cccaagagaa tttacttgat
     3121 atctccgggg tatccgccaa tcctcgagcc ggaaatcttg agactccaca gatttcacaa
     3181 gttattcaca acaatgaccc cttggctaac cgatccatcc gatattatcc acagtatcca
     3241 caagttatcc acaggaaggg ctgtatataa cccgccatca tatagaggat tcatcaaccg
     3301 gttcctggat gaacaggtca agttatcaac agcctgaaga aatcggtgga aaaattcgaa
     3361 attaggccaa tcaacccggt gatgggagaa caaagccgct caaagcacca aatccggttg
     3421 cgcctgtacc tcgattccgg tccgatcccc atgggaataa tgaaagtatc ctgcggcggc
     3481 gatcatagcg gcattatcgg tgcataaccg aggttcggga tagtacacct tctttcccaa
     3541 acgatccgct tcctgctcac agcgacttcg cagataagag ttggccgcga ctccgccgga
     3601 aagcagtatg gtatcgactc cataatcacg gaccgcgaga aatagtttgc cggtcagttg
     3661 ttccacagca atccgttgaa agctcgccgc cactctgggg atgtcaatct cccggccggt
     3721 ctgtttctga tgatgcagat aattcaggac cgctgttttc aaaccgctaa aagagaaatc
     3781 gtaactattg gccaatccac gtagctgtgc aaactccaga tccgccggta ccccccgggc
     3841 aatcctgtca atatagggcc caccgggata tcccaatccc aaagcccggg caatcttgtc
     3901 gaacgcctcc ccggccgcat catcccgggt ggatcccaga atacgatagc tgccccattt
     3961 ctcgatgaac aaaagatcgg tatgacctcc ggagaccgtc agacagatca aaggcggctt
     4021 aagctccggc cgttccagga cattggcata aatatggccc tcaatatgat gaacaccgat
     4081 aaagggaatc cctgcggcaa ttgccaatcc tttggcgaac gaaagaccca ccaacaggct
     4141 tccgacaagt cccggcccat aagtggccgc taccagacgg atctgctcaa gtttaactcc
     4201 tgcttccgct agcgccattt ggtatatggg cagcaccgcc tctaaatggc agcgggcagc
     4261 taactccggt acgaccccgc cgaatttggc gtgcagatca atctgagagg agatgatatt
     4321 gcttcgaatc ttgaaaccgt cggcaaccac tgccaccgaa gtatcatcgc aggagctttc
     4381 cactcccaaa gttagaaatt cagccatttc ccccacccgt aactccgcct ttcttcccgc
     4441 gacaccatac cctttgggaa tggaacctgg gcccaactca tcctcagcat tcccaagagt
     4501 ctgtgagaaa tgccaatcca atcttaaaaa aaagacccta taaaggtctt tttttgatcc
     4561 atttgtagtt cgacacgtgt caggcggaat gttgctcctc cgtggccagc gccagagccc
     4621 ggcgcagatt acccgatgat tcactggttt tctcggaaat ccgtaccgcc tctttcaaat
     4681 tcaactggta gtccaagatc tccaaatcga tattttgcag ttcgtctttt tccgtagccg
     4741 gaaccccggc gatgacattg gcctcttgga tgatggtttc gcttttggcg ataatctctt
     4801 caaggacctg gaccagttga agcatattat cgacctcgga atgcgcggca gtaaagaccc
     4861 gattgctgat ttcccccgca tcccgacgca actccccgag gagctctatg ttgacccccg
     4921 cctggggcaa ctcttcccgg agactctctt gtctggcttg gatcgcgttc accgcagcat
     4981 gtaaatcttc ggtagctttt ttggatcgct ccactaattt ggtgatctcc tgtgaaacga
     5041 caccgaatcc caaccctttg tttccagcgc gggccgcctc aattgcggta ttgaaagcca
     5101 gtaactcggt ctgttcggaa accccgttca atacccccat caccgaatca accttggtcg
     5161 acatctcagc caggttatgg aagagatcga gaattaacgg ggtacagttt tcgatagtaa
     5221 cctgacgatt gagcaactcc tccatttgac cttccgcctg aataactacc ccatcgagtt
     5281 cctcggcgaa ctgccccaac tgttcgggaa tgcgttgcag ctcggtgatg ttggaaatga
     5341 tgaaggtaag gtgttcgttg gatttttgca aggtttcgtt ctgttcattg atatacgtat
     5401 caatactctt ataaacttca ttaaactgaa tctcggatat ctcccgaagt ttcacctgac
     5461 cgaccaactc ttggcaaagg ttttccaatt tcgctgtgga attcttcagt ttctcaaaga
     5521 tttcatccca tgaacgaaat ccctcattca acaaaagggc ggcgactcca agaccgtccg
     5581 gtgaataagc gggaatatga ttcgcggccc cggccagccg gtcctggttc aattgttccg
     5641 tgataagcac agccggttcg gttatggtcc ggactgcggc aatcaccagc cagccgctta
     5701 ctacgaccat taaaaccaag atcaccagga ccagtagggt aaacccggcc atcctcagaa
     5761 gcatcccccg gtttcgattg ctatagaccc ggttgagcgc agtttcagtt tgaatcaaag
     5821 cattacggta ttgagaatac aaacgcttga cggatccata ctgagccgaa tccagattcg
     5881 cgccgttata atatttgagt tgctcccgga gcgcttgaaa acgattatgc aatgaatcgg
     5941 tattcatatt gtcggacttg acaatacggg cgatcccggt ttcgatccgg gcaagagagt
     6001 ttcccagttc caccggttga cccgtcttca ccgcaacggt acaacgggtt aatagatcgg
     6061 gagacccctc cttgatggtt ttcaaggtat tgagttcctg tttcatttga ttcagataac
     6121 gaccttcggc tgcaatctga aaataccgtg aaaaagccca aaagccctga agtatcagtc
     6181 ccagcaataa aactccaaag gccaaaaaga tcttggtttt gaaattaagg ttggatagaa
     6241 ttccaacccc atgatacgct tcaactttag ggaagaattt ttgcaggtat tttatcatcg
     6301 aaactcctcc gcctaaggca ctccggttta ttaacaccag atgcgctaat tctccatctt
     6361 cgccaagagt tggttgactt cgataaccag attttcaagc tcactcatgg aaagtgggaa
     6421 atgatcgggc ttatccacgg catccagggc ggtcgccgcc ttactataat cctccggaat
     6481 acgacggttc agttccgcta aaaacccggt cagctcttcc agcgataaac gccggacttc
     6541 atattgatgg accagggatt cgaattcttg aatctgctcc aacattacgc tgaacttttc
     6601 ccaaaaagcc gccagctggg tttcaaatgc ggccaaatac tgtttcactt gattgttttg
     6661 gggcaataac ccctcatatg tatgtatcgc ctgttcagtc tgtttctttt ctttattcaa
     6721 cagcagcaat aattcgtgag gaattttccc gatccggtcc gccaattcac cgatttcggt
     6781 ggcggcgatg tcaaagccgt cgccggcagt gccggcccgg gcggcttcaa tggcggcatt
     6841 caaggaaagc accgaagtct gctcggtcaa accctcaaaa accgtaacga tctgctggag
     6901 ctgatccatc atgactcgca gttgttttaa gcttgcgaca atctgtgcgg aattattggc
     6961 cctgagatcg agcttggagt ttaaccggtt tacctccgaa gagcccacct tgataacctc
     7021 tccggcttga gccag
//
LOCUS       DASVMB010000519         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_652885,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000519 DASVMB010000000
VERSION     DASVMB010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652885"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1345)
                     /locus_tag="VF531_14610"
     CDS             complement(29..1345)
                     /locus_tag="VF531_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715245.1"
                     /translation="MDIANRYFYSVGYQQKSLEALETALGTAPLYKDWRAFDPGSGTP
                     LDQRYAALPELTKKAMREHFPQGLVPNQRRVEDGLARNEIEYTFTSGTTDEKVVNFWN
                     QTWWYSSEAASWKLNAHTARLDHPQKEAKLASALNVGIQCEEDLPMDHRILGRHLFLN
                     EKISVLQWQPRHLERMARELKIYQPVILEANPSLLARLAWWALDNGQELYSPAAIIFT
                     FEYPSQIHLAAIRRVFSSPLVSSYGTTETGFVLMQCEAGLFHQNTNFCRIDFEPLAAR
                     HGGPDLGRILVTTFNNPWTSIVKFDAGDLIRLHPTDKCACGRNEGLLVEAIEGRVANA
                     TFTTRDVLVTTMALDTQLAQIPEIRDYHLEQQSRTRYELQLVLTDSPANNRVEVLDRT
                     RQALESLYGGDGEYILVPVSDLLPGPAGKYRRTQANFDFDQKGLFA"
     gene            1523..2107
                     /locus_tag="VF531_14615"
     CDS             1523..2107
                     /locus_tag="VF531_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7715246.1"
                     /translation="MIHNEVLDCIRVRRSTRQFQQRQIGEEDLKALLEAAIWAPSGGN
                     NQSWLFTAIQNKDILLKINELVREGFGRWIPDDDYPGKLAAKTNAQKKDYNFYHHAPT
                     LIIASNKPNYENAMADCALALENLFLAAQSLGLGSCYINQLHWLRNDAELRNYLLELG
                     IPKEHTICSSAAVGYIAKESNPPLRKDGTVNIIK"
     gene            complement(2204..3001)
                     /locus_tag="VF531_14620"
     CDS             complement(2204..3001)
                     /locus_tag="VF531_14620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7715247.1"
                     /translation="MDYHAVLKQVESWLMEVGTEQLEGLGRKLSITTKSSEFDPVTDV
                     DRKSEEFLVGAIQHHYPEHSILTEERGAMENGSGYRWVIDPLDGTVNYAHRFPIFGIS
                     VALQHHGQTMLGAVYFPVLKEFFHAILGEGAFLNDRRIAVSPITELSKSLLATGFPYD
                     RATDPDNNLNYFNHLMLQIGGISRSGSAAFDLCNVACGRINGYWEFKVHLWDVAAGDL
                     IVSEAGGKVDYLECRKGVALIAGNGKINESIYRELTKVNPAVKRIEE"
BASE COUNT          813 a          887 c          788 g          793 t
ORIGIN      
        1 gccaacaaaa aaaccggttg gggtccgttc atgcgaataa ccctttttga tcaaagtcga
       61 aattggcctg ggtccggcgg tatttccccg cagggccggg caggaggtcg gagaccggta
      121 ccagaatata ctcgccatcc cctccatata aggactccag agcctggcgc gtccggtcca
      181 ggacctcgac ccggttattc gccgggctgt cggtgaggac caattgcagt tcataccggg
      241 tccggctctg ctgttccaag tgataatccc ggatttcagg gatttgggcc agttgggtat
      301 ctagggccat ggtcgtcacc aagacatctc gggtggtgaa agtggcattg gccacccggc
      361 cttcaatggc ctccaccagc aagccttcat tccggccgca agcacacttg tccgtggggt
      421 gcaaacggat cagatccccc gcatcaaact tgactatgga agtccaggga ttgttgaaag
      481 tcgtgaccag aatccggccc aaatccggtc cgccatgtct ggcagcgagg ggttcgaaat
      541 caatccggca gaaattggtg ttctggtgaa acaggccagc ctcgcattgc atcagcacaa
      601 agccggtttc cgtggtgcca taggaactga cgagcggcga cgaaaacacc cgccggattg
      661 cggccagatg gatctgggag ggatattcaa aggtaaagat gatggcggcc ggggaataaa
      721 gttcctgccc attgtccaat gcccaccagg ccagacgggc caaaagcgaa ggattggctt
      781 ccaggatgac cggctggtat attttcagct cccgcgccat ccgttccaaa tggcggggct
      841 gccattgcag cacgctgatt ttttcattca gaaacaaatg acgtcctaaa atacggtgat
      901 ccatcggcag atcttcttcg cattgaatcc ccacgttcaa ggcactggca agtttcgctt
      961 ctttctgggg gtgatccagg cgcgcggtat gggcatttaa tttccaggaa gccgcctctg
     1021 agctgtacca ccaggtttga ttccagaaat taaccacttt ttcatcggtg gtgccgctgg
     1081 taaaggtata ttcgatttcg ttccgggcca gtccgtcctc aacccggcgt tggttgggaa
     1141 ccaacccctg gggaaaatgt tcccgcatgg cttttttggt cagttccggc agggccgcat
     1201 atctctggtc gagtggggtt cccgatccgg gatcaaacgc ccgccaatcc ttatatagcg
     1261 gtgcagtccc cagagcggtt tccagagcct ccagcgattt ttgctgatac cccacagaat
     1321 aaaaataccg gttagcaata tccatgtgca cgcctcccca tttcaatgcc ttcatcttct
     1381 tactatttct ggtaagaaga ttgtaccacg aacgtatgtt tggatcaacc cttttgcaaa
     1441 aacatagcta aaatttatta tggtataata aaatgtgtta acttacggcc aaatatgcct
     1501 tatcgtggaa aggaacgaat gaatgattca caatgaagta cttgactgta tccgtgtccg
     1561 caggagcaca cgtcaatttc agcaacggca gattggggag gaagacctga aagccttgct
     1621 tgaagccgcc atctgggcgc ccagcggagg caacaatcaa agctggctgt ttacggcaat
     1681 ccaaaacaaa gatattctgc taaaaatcaa cgaattggta cgggaagggt tcgggcgttg
     1741 gattcctgat gacgactatc ccggcaaact ggcagctaaa accaatgcgc aaaaaaaaga
     1801 ctacaatttc taccaccacg cgcctaccct gatcattgct tccaacaagc ccaattatga
     1861 aaacgccatg gccgactgcg cgctggccct ggaaaatctc tttttagccg cccaatcgct
     1921 ggggctgggt agttgttaca tcaatcaact gcactggttg cggaatgatg ccgaattacg
     1981 caactatcta ttggagttgg gaatccccaa agaacatacc atctgttctt ccgctgcggt
     2041 agggtatatt gcgaaagagt caaaccctcc tttacgcaag gatggcaccg tcaatattat
     2101 aaaataagca tgaacatttt cccttaacac tccaaataaa aaaacatccg aaaccggtgc
     2161 atttatccta tttaaacttc aattccatat gaaaagataa atgttattct tctatccttt
     2221 tcactgccgg attaaccttt gtcagctccc ggtaaatcga ctcattgatc ttcccgttcc
     2281 cggcgatgag ggccactccc ttcctgcatt ccaaataatc caccttcccc ccggcctcgc
     2341 tcacgatcaa atctccggct gccacatccc agagatgcac cttaaactcc caatatccgt
     2401 tgatccggcc gcaggccacg ttgcacaggt caaaggccgc gctgccgctc cgggaaatac
     2461 cgccgatctg cagcatcagg tggttgaagt agttcagatt gttgtcggga tccgtcgctc
     2521 ggtcgtaggg aaaaccggtt gccagcaacg acttggagag ctcggtgatg ggcgagaccg
     2581 cgatccgccg atcgtttaaa aaggcccctt cccccaggat tgcatgaaaa aactccttaa
     2641 gcaccggaaa atataccgcc ccaagcatcg tttggccatg gtgctgcaag gccacggaga
     2701 tcccgaagat tggaaagcga tgagcgtaat tcacggtgcc gtccagcgga tcgatgaccc
     2761 accggtaccc ggaaccattc tccatggcgc cccgctcctc ggtgaggatc gaatgctcgg
     2821 ggtaatgatg ctgtattgct ccgaccaaaa attcttccga cttacgatcc acatccgtca
     2881 ccggatcaaa ctccgaggac ttggtggtga tcgaaagttt tcgtcccagc ccctccagtt
     2941 gttctgtacc cacttccatc agccaggact cgacttgttt cagtaccgcg tgataatcca
     3001 tcgtggtgaa gtgcctctct ttaagtagtc gttttgtagc tagtttaaac tgtataaata
     3061 aaagtttacg atgaattttc caggaatcct tgttaaatat ttcgtaacgg tagaatactt
     3121 atatcgaaaa aggcgcccga aacgggcgcc tttctatcgt ttagtcttat atttcattga
     3181 agtgtcggac tcagtcccta ttttaatccc cgatcaaccc cggccatttc cctgcagatt
     3241 gcctcgttga tccttccgtt cccggcgatt aaggctaaac c
//
LOCUS       DASVMB010000520        12064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_360690,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000520 DASVMB010000000
VERSION     DASVMB010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360690"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /gene="cgtA"
                     /locus_tag="VF531_14625"
     CDS             <1..818
                     /gene="cgtA"
                     /locus_tag="VF531_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX7715248.1"
                     /translation="VDLELKLLADVGLIGFPNAGKSTLLSRISAARPKVADYPFTTLI
                     PNLGVVNVKGQSFVAVDIPGLIEGAHSGAGLGDQFLRHIERTRLLVHLVDVSGISGRD
                     PYQDYLQINEELRLFDPELATRIQIVVANKVDLPDSKSILESFIARIGDCRVIPISAA
                     TGEGVSDLLDRVLYLLTQIPKESSQLIVSEPEIDTESLAIQVLKIESGFMVQNDGLLR
                     RMARFDLNNEESLRSLQRLLKRWGVIQALIDAGVQEGDMVRIGDFEFTYLSEE"
     gene            970..1161
                     /locus_tag="VF531_14630"
     CDS             970..1161
                     /locus_tag="VF531_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715249.1"
                     /translation="MLCLDCGNCRKGDPVFYCTARNEFIATETSIVRERGEERWRKGD
                     PRYEHHRRQLRKDKIPQVG"
     gene            1469..1771
                     /locus_tag="VF531_14635"
     CDS             1469..1771
                     /locus_tag="VF531_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX7715250.1"
                     /translation="MLTSKQRSFLRALGNEMEPILTIGKGGISATVIEQLDQALSARE
                     LIKARVLPHTEYESRSIAEEMATAINCEIVQVIGRNILFYRQAQPGVSSKIPWDQM"
     gene            1835..2965
                     /gene="proB"
                     /locus_tag="VF531_14640"
     CDS             1835..2965
                     /gene="proB"
                     /locus_tag="VF531_14640"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7715251.1"
                     /translation="MDSHSVHIKAARRVVVKVGTSTLTHHSGKLNLNRMEQLVRQLVD
                     LQNQDKEVVIVTSGAVGAGMGRLGITQKPASIMEHQALAAIGQGLLMQVYEKLFAEYG
                     QTVAQVLLTRSDVSDRKRYLNARNTLLTLLKYRVIPIINENDTVATEELKFGQNDCLS
                     ALVAGLIEADLLVLLSDIDGLYTADPKTDPNATLIPLVTEIGPEILAMAGGAGSKLGT
                     GGMITKMEAARMATAAGTSMVIMNGNDPAKIQYLFAGTPVGTVFLSNQPLVNSRKRWI
                     AFGPPVTGELMVDDGAERALLKQGKSLLPSGIVTLSGEFEEGDLIRIVNCSHQELGRG
                     LTNYSKEQLEKIRGRHSSEIQSILGFKFTDEAVHRDNLVITA"
     gene            3291..4541
                     /locus_tag="VF531_14645"
     CDS             3291..4541
                     /locus_tag="VF531_14645"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX7715252.1"
                     /translation="MDTATYIEELGRNAKKAAPILARLSSTQKDGLLLAMADRLEKGT
                     DRILAANRLDLDSARQNQRQVLLDRLVLNEKRVRDMADGLRTLVNLADPIGQTIRMWR
                     RPNGLEIGEQRVPLGVVGIIYEARPNVTVDAAGLCLKTGNAVILKGGSEALESNRVLV
                     ELLREVCRETGIPMDALQLVERTDHQAAQVLMRQNKYLDVLIPRGGAGLIKTVVENAT
                     VPVIETGVGNCHTYVDRDADIEMACRIAFNAKVQRPAVCNAMETLLVHQDVAQVFLPA
                     IAAKYESAGVQLRGCPKTCSLIQAQPATENDWETEYLDLILAVKVVESMDEAIAHINR
                     YGTRHSECIVTNNYPNARRFLDEVDAAAVYVNASTRFTDGFEFGFGAEIGISTQKLHA
                     RGPMGLEALTTIKYIIYGSGQIRG"
     gene            4587..5231
                     /gene="nadD"
                     /locus_tag="VF531_14650"
     CDS             4587..5231
                     /gene="nadD"
                     /locus_tag="VF531_14650"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7715253.1"
                     /translation="MGYFGIMGGTFDPIHLGHLILAEKAGEVSSLEKIIFVPAAIPPH
                     KIGEVLTPAEHRLRMVELAIEGHPGFEISRVELDREGPSYTADTIRQLIAIERGAEAV
                     LILGFDSLLELYTWKNYRDILDQARIITAFRPGSPVLRDANDWPLFLQPYRERITILE
                     APLLDISSTWLRVELMQGRSIRYLVPDAVREYIRENRLYEPRSGSTYSFFEKAQ"
     gene            5446..5706
                     /locus_tag="VF531_14655"
     CDS             5446..5706
                     /locus_tag="VF531_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7715254.1"
                     /translation="MDTRTLYVGNLPWSATKEDLAALFSNYAQVRSSRIITDRASGRS
                     RGFGFIEVEDDNVEQIIEKMNGKDMGGRELIVNEAKPRQAKM"
     gene            5902..6393
                     /locus_tag="VF531_14660"
     CDS             5902..6393
                     /locus_tag="VF531_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX7715255.1"
                     /translation="MANEASFDVVSKVDLQEVDNAVNQTVKEIGQRYDFRNSLAEITL
                     NGEELKISADNDYKLKSIIDILQTKLIKRGVSIKNLEYAKVESASGGTVRQMIRIKQG
                     IATETAKQMVKDIKTLNLKVQAQIMSDQLRVSGKSRDDLQAVIQALKAKDYGVELQFT
                     NYR"
     gene            6522..7094
                     /gene="yqeK"
                     /locus_tag="VF531_14665"
     CDS             6522..7094
                     /gene="yqeK"
                     /locus_tag="VF531_14665"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEX7715256.1"
                     /translation="MNRNEIVNYLQNRLSETRFKHCLGVESVCLKLAARFGVDPEHAS
                     FAALLHDLCREYDRDSLLKLAVNFGIVVDDIEKSEPLLLHGAVGAGLARRELEIEEPA
                     ILEAITYHITGAAGIGPLARLIFVGDFIEPGRTFEQARILRDEADKLAPEQLLLMVYN
                     RSITYVINRNYLLHPRSLEGRNELIHKGIR"
     gene            7100..7435
                     /gene="rsfS"
                     /locus_tag="VF531_14670"
     CDS             7100..7435
                     /gene="rsfS"
                     /locus_tag="VF531_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7715257.1"
                     /translation="MEPLLELIKEAALEKKALEPMLLDLRGISGITDYFFICSGNTQV
                     QIRAIADAITDKLEAADIPLPPKEGYRDGRWILLDCGSLVVHILNQQEREFYALENLW
                     HDAKVIPVQ"
     gene            7556..10009
                     /gene="leuS"
                     /locus_tag="VF531_14675"
     CDS             7556..10009
                     /gene="leuS"
                     /locus_tag="VF531_14675"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7715258.1"
                     /translation="MGKVDKYHHREIEPRWQNKWEETGLYHSDIADRPKYYAMTMLPY
                     PSGDLHIGHWYAMTPSDAHARFKRMQGYNVMFPMGFDAFGLPAENAAIKRGVHPYKWT
                     MSNIERMRQQLKSMGALFDWRREIVSCLPGYYRWTQWFFLKFYEKGIAYKKFAPVDFC
                     PQCNTTLAREQVWGEDRHCERCETPVVKRELNQWFFKITDYAEELLDFSGMDWPERVV
                     TMQQNWIGRSEGAEVVFHTEKGQEMRIFTTRPDTLWGVTFMVLAPEHPLVDELTTSEY
                     RAQVEQYKHQASRQSEIERLAVTKEKTGVFTGAYAINPVNGSKVPIWTADYVMMSYGT
                     GAIMAVPAHDERDFAFAKKYQLPIRMVIAPSGINLDHPLPEAYTSVEEGLLVNSAQFD
                     GTPAKEAKQQVTKWLEEKGFGKKAVNYRLRDWLISRQRYWGAPIPIIYCEKCGTVPVP
                     YEDLPVLLPEDAEFLPTGESPLKYHEGFRKTTCPRCGGPAERETDTMDTFMCSSWYQY
                     GYLSPYYKGDVPFDPKEAAYWLPVDLYTGGVEHACMHLIYTRFFTKAMRDIGLVDFDE
                     PMLKLRNQGIILGEDSEKMSKSRGNVVAPDDLVQKYGADAVRAYLMFGWRWEQGGPWD
                     SRGIDGMYRFMNRVWELTLEEMKPLSAEPADAAKSLVRKTHQTILKATRSIEDFSFNT
                     YLASLMEFSNVMAKYKTELYASDAWNEAVKSLLLLLAPACPHITEEIWSRLGYGYSIH
                     NQPWPKYNPELAAEETVEIVLQINGKIREKLNVPVNSSEEDLKDLALKNQAVEKAIAG
                     KTVKKVIVVPNRLINVVVA"
     gene            10120..10674
                     /locus_tag="VF531_14680"
     CDS             10120..10674
                     /locus_tag="VF531_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7715259.1"
                     /translation="MEEQIRLFWIPIFLQPEERRTVKATFSSGNCVIQFPRQMLGRKT
                     QLLTTLDHVYWRLMGELALPDLKQRTAELNQAFFKFHYRSVRYHRQFRRWGSCSDRGN
                     INLSHRLIGGPRELVDYVILHELAHLQYLNHGPDFWRLVRSTGEEPQQIRKAMTVYGR
                     EWQYHYENWYRRLHQIIQSPKPKK"
     gene            10831..>12064
                     /gene="spoIVB"
                     /locus_tag="VF531_14685"
     CDS             10831..>12064
                     /gene="spoIVB"
                     /locus_tag="VF531_14685"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HEX7715260.1"
                     /translation="MCALKNKPLQFILFFALFLLGSSVIAGLIEYMNLPGTLHLSPGQ
                     KITLSVHYPLSFHSGSSLTFQESLKLSAFDHLLTKELVLSPKSAERFEVQIRIFGRIP
                     VKKLQVEVAEPPQLIPGGQAVGVLFSSKGVVIVGHLPIKGVDGKMYYPAKTAGLKVGD
                     VLLAINSLPVNRVDDVEVLLRNFRPWYRTLNLTIKRGNQIRLIKVHPVLSYEEGTQHQ
                     RYMLGIFIEDPAAGVGTLTFYDPVTRKFAGLGHHIAEFSGAKGLTLQNGEIVLASING
                     IKAGLPGQPGEKIGVCSTNLSPIGLIEKNSRFGIYGTLTENFIDFTKARSLPMAYSSQ
                     ITLGSAEIYTVIKERLVEKFQVQIIKVYRQDSPRDKGLILKVTDPELLKRTGGIIQGM
                     SGSPIIQNGRLIGAVTHVF"
BASE COUNT         3198 a         2630 c         3135 g         3101 t
ORIGIN      
        1 gggttgacct tgagttgaaa ctcttggccg atgtgggatt gattggattt ccaaatgccg
       61 gtaagtccac attattatcc cggatatccg ccgcccgacc gaaagtagcc gattatccgt
      121 ttacaacgct catccccaac ctgggggtag tgaatgtgaa aggccaaagc ttcgtggcag
      181 ttgatatccc ggggttaatt gaaggagcgc atagtggagc cggactcggc gatcaatttc
      241 tccggcatat cgaacggact cggcttttag ttcatttagt ggatgtttcc ggaatttccg
      301 gccgcgatcc gtaccaggat tacctgcaga tcaatgaaga gttaaggctg ttcgatcccg
      361 aactggccac caggatacag attgtagtcg ccaataaggt tgatcttccg gattccaaat
      421 cgattctgga atcatttatt gctcggatcg gtgattgtcg ggtgattcct atatccgctg
      481 ctaccggtga gggagtatcg gacttgttgg acagggttct ttatctgctg actcagatcc
      541 ctaaagaatc ttcacagtta attgtatccg aaccggagat tgatacggaa tctttagcta
      601 ttcaggtact taaaatcgaa agcggtttta tggttcagaa cgatggtttg ttacgccgga
      661 tggccaggtt tgatttaaat aatgaagaat ctctccgtag cctgcaacgg ctcttgaaac
      721 gctggggcgt aatccaggcg cttattgatg ccggagtcca agaaggcgat atggtacgaa
      781 ttggtgattt tgaatttact tatctcagtg aagaatgact ttcaaatccg ggtgagcgta
      841 tttgtggtta agattcccac actttgaaaa ctcttaaata aaaccatcgg cgaaacgccg
      901 ctcgtcacac gacaatcatc gaaatggtcg ttacgataaa aatagagtga ggattaagga
      961 ggttttaaag tgctctgtct agactgtggg aactgtcgga agggtgaccc ggtgttttac
     1021 tgtacggcac gtaacgagtt catcgccacc gagacatcta ttgtacggga gcggggcgag
     1081 gaacgctggc gaaagggtga tccgcgatac gagcaccacc ggaggcaact tcgaaaagat
     1141 aaaattcctc aggtgggtta gggttgccat ctggcgccat tcaccgtccc gcaaacgagc
     1201 gccgaatctt acaccggtgg attggccaaa ctttttcact ggattttaag tggagcctag
     1261 cttcactttt tttattttcg ctgatcccaa atcttcacga agagccgaga agtcgcatca
     1321 ccagggcagg aaatctttta aagttcaatt cccctccaaa agaccaaaag gttgaaaaag
     1381 tctcaaacct ccgtgtaaaa tatgctataa tgtttccaaa ctgaattgga tagatcaaac
     1441 cttttcaaaa gggaagaagg tcgtagcgat gttgacgagc aagcaacgaa gtttcttacg
     1501 ggctttaggc aatgaaatgg aaccgatttt aaccattgga aaaggcggaa taagtgctac
     1561 cgtcatcgaa cagctggatc aggcgcttag tgcccgtgag cttatcaagg ctagagtttt
     1621 gcctcatacg gaatatgagt ccaggtcgat tgcagaggaa atggcaacgg caatcaattg
     1681 tgagattgtg caagttatcg gtagaaatat acttttttac cgacaagccc aacccggggt
     1741 atccagtaaa atcccgtggg atcagatgta aaacccgatg tttcagactg tttgacggtt
     1801 tggcgataag taaaccattt tcaggaggaa aacggtggat tcccatagcg tgcacatcaa
     1861 agccgcccgc cgggttgtgg ttaaggttgg taccagcacc ctcacccacc acagcggtaa
     1921 attaaattta aaccggatgg aacaattggt ccggcagtta gtcgatctgc aaaaccagga
     1981 taaagaagtg gtgatcgtaa cctccggtgc tgttggcgcc gggatgggcc gtcttggtat
     2041 cacccaaaaa ccggcatcga tcatggaaca tcaggcattg gctgccatcg gtcaaggatt
     2101 attgatgcaa gtttatgaaa agctttttgc cgagtatggt cagaccgtag cccaagtttt
     2161 attgacccgt agcgatgtga gtgataggaa acgctatcta aatgccagaa ataccctttt
     2221 gacattgttg aaatataggg taatccccat catcaacgaa aatgacacag tcgctacgga
     2281 agagttgaag tttgggcaga acgattgcct ttcagcctta gtggcagggt tgattgaagc
     2341 ggatctcctg gtactgcttt cggatattga cggtttgtat accgcagatc caaagaccga
     2401 tcccaacgca accttaatac ctctggttac ggagattggt ccggaaatcc tggcaatggc
     2461 cgggggggcc ggatcgaaac tgggaaccgg cgggatgatc accaaaatgg aggctgcccg
     2521 gatggcaacg gcggccggaa cctccatggt gattatgaat ggaaatgatc ccgccaagat
     2581 tcagtatctt tttgccggga ccccggtggg caccgtcttc ttaagtaacc aacccctggt
     2641 aaacagtaga aaacggtgga ttgccttcgg acctccggta accggcgagc tgatggtgga
     2701 cgacggagcc gaacgggctc ttctcaaaca gggtaaaagt ttattaccct cgggaattgt
     2761 aactctttcc ggtgagtttg aggagggcga tctcatccgc atcgtgaact gcagccatca
     2821 agaattaggg cggggcttaa cgaattattc caaagaacag ctggaaaaga ttcgcggccg
     2881 gcattccagc gagattcaat cgattttggg ttttaagttt accgatgaag cggttcaccg
     2941 cgataactta gttataacgg cataaattat tttgtggatt tcgtgtgttt ctgagttcaa
     3001 aagatttaga aagatgccgg ataaaaaggg cggatctatt taaccacaaa atttatgaaa
     3061 gaacacgaaa agtttttaga gggttctgaa tgcttttttc gtggattttg tgtatctcgg
     3121 agttaagaag atttagaaag ataccgggta aaaagggcgg atctatttaa ccacgaaatt
     3181 cacgaaagga cacaaaaagt ttttagagag ttccgaatgc attttttcgt gtgtttcgtg
     3241 tgtttcggag ttaagatggt tttaccaagt caagtattgg gggttgagaa atggataccg
     3301 caacgtacat tgaagagctg ggacggaatg caaaaaaagc agcgccaatc ttggcgcggc
     3361 tttcgagcac ccaaaaagat gggttgttgc tggcgatggc ggaccggctg gagaaaggga
     3421 ccgatagaat tctcgcagcc aaccgtctcg atctggactc ggcacgtcaa aaccaacgtc
     3481 aagtattgct ggatcgtttg gttttgaatg aaaaacgtgt tcgagatatg gcggacggtt
     3541 tacgaaccct ggtcaatctt gccgatccta tcgggcaaac gatccggatg tggcgtcgtc
     3601 ccaatggcct tgagattgga gagcaacgtg ttcccctggg agtggtgggg attatttatg
     3661 aagcccgtcc taatgtaacg gtggatgccg ccggtttatg cctaaagacg ggaaacgccg
     3721 tgatcttaaa gggtggatcc gaggccctgg agtcaaaccg ggtattggtc gagctgctgc
     3781 gagaggtttg ccgggagaca ggcataccta tggatgccct ccaattggtg gaacggactg
     3841 atcaccaggc agcccaggtt ttgatgcgac aaaacaagta tctggatgtc ctaattcccc
     3901 ggggtggagc ggggttgatc aaaaccgtag tcgagaatgc cacagtgccg gtgattgaga
     3961 ctggagtagg caattgccat acttatgttg atcgggatgc cgatatagag atggcttgcc
     4021 gtattgcttt caatgccaag gtgcagcgcc ccgcggtctg taacgccatg gagacattac
     4081 tggttcatca ggatgtggcg caggtttttt taccggcaat cgctgccaaa tatgaatcgg
     4141 caggcgttca acttagggga tgtccgaaaa cctgttcact cattcaagct caacccgcca
     4201 cggaaaacga ctgggagact gaatatttgg atctcatctt agccgtaaag gtggttgaga
     4261 gcatggatga agccattgcc cacatcaacc gatacgggac ccgtcactcg gaatgcatcg
     4321 taaccaataa ttatccaaac gccaggcgtt ttttggatga agtggatgca gctgcagtct
     4381 atgtaaacgc ttcgactcgt tttaccgatg gcttcgaatt cggattcggt gcggaaatcg
     4441 gaattagtac ccaaaaactt cacgcacgcg ggccaatggg gctcgaagct ttaactacca
     4501 ttaaatatat tatttatggc agcggccaaa ttcgtggctg aaatctgtca ctgaccggtc
     4561 ttccatgcga gaaagggcgt gaggtcatgg gatattttgg tatcatggga gggacgttcg
     4621 atccgatcca tctcggacat ttgatacttg ctgaaaaagc cggcgaagtt tcttcccttg
     4681 aaaaaatcat ttttgttcct gcagctatcc cgcctcataa gattggagag gttttaaccc
     4741 cggctgaaca ccgcctgcgt atggtggaac tggccataga aggtcatccg gggtttgaga
     4801 tttccagagt tgaacttgac cgtgaaggcc cttcttatac cgcggatacc atccggcaat
     4861 tgatagccat cgaacgggga gccgaagccg tcctgatttt gggatttgat tcattactag
     4921 agctttacac ctggaaaaat taccgggata tcttggatca ggccagaatc atcacggcct
     4981 ttcgtcctgg ctctccggtg ttgcgtgacg cgaatgattg gccgttattt ttacagccat
     5041 atcgggaacg cattaccatc ctggaagctc ccctattgga tatctcctca acctggctac
     5101 gggttgagct gatgcagggt cgttccatcc gttacctggt tcccgatgct gtcagggagt
     5161 atattcggga aaaccggtta tatgaacctc gatccggtag tacatatagt ttttttgaaa
     5221 aggcgcaata gcattagcga gtcggcacgc gtgaatgcac gatatgaaat aaaccggtgt
     5281 tagcagtaac ttgccggatt cgatttgacc tgacttatca agatttaagt ggctataccg
     5341 aacggggggt gtttttaaac ctgtactggt atagtccttt ttttttgatg cacaatatta
     5401 gtaagcgcgg gtgcgttaac tcaaagtaga ataaggtgga ggaatatgga tacgagaact
     5461 ttgtatgtcg gcaatttacc ttggtcagcc acaaaagaag atttagctgc gcttttttcc
     5521 aactatgccc aggtccgttc aagccggatc attacggatc gggcgagcgg gcggtctcgc
     5581 ggttttggct ttatcgaggt cgaagatgac aatgtcgaac agatcattga aaagatgaac
     5641 gggaaagaca tgggtgggcg ggaattaatc gtcaatgagg ctaaaccccg tcaagccaag
     5701 atgtgagtat ccttagatgt tcagatgtta attggaaggg tgttttgcgg catgacccaa
     5761 agcactcttt tttattatca tcattgcttt tttaacttct tatcaaaaaa tggagggaaa
     5821 tcattccggg acgagaattg tacattgatt tccccgtgcg tgcgcgctaa taaaccgggt
     5881 attaccaagg aggaatggca aatggccaat gaggcttcgt tcgatgttgt ttccaaggta
     5941 gatttgcagg aggtggataa tgccgtaaac caaaccgtaa aggagatcgg gcagcgttat
     6001 gacttccgta atagcctggc agagatcacc ttgaacggcg aggagttgaa gataagcgcg
     6061 gataatgatt acaaactaaa gagcatcatc gatatccttc agacaaaatt aatcaaacgc
     6121 ggcgtttcca ttaagaatct ggaatacgcc aaagtggaat cggcaagcgg cggcaccgtt
     6181 cggcaaatga tccggattaa gcaggggatc gcaaccgaga ccgccaagca gatggtaaag
     6241 gatatcaaaa ccttgaatct taaggtccaa gcccaaatta tgagcgatca gctccgggtc
     6301 tccggaaaaa gccgggacga cctccaggcc gtcattcagg ccttaaaggc gaaggattat
     6361 ggggtggaac tgcaatttac caattaccgt tgaccttagt ctgaccttta tctcgttccg
     6421 aacatattat ataaacctga ttcaacggat ctcctctgct ttcggataaa atttcgaatg
     6481 gtccgaatgc catgaatcgg tttggaaacc gggtgacttg catgaatcgt aatgaaattg
     6541 taaattatct tcagaaccgg ttatcggaaa cccgctttaa gcattgcctg ggtgtggagt
     6601 cggtctgcct gaaactggcg gccaggtttg gggtcgatcc ggaacatgct tcttttgccg
     6661 cgttacttca cgatctctgc cgggaatatg atcgggattc tctcttgaaa ctggcagtta
     6721 attttggtat agtagtagac gatattgaaa aaagtgagcc tctgctgtta cacggggctg
     6781 ttggtgcagg tttagcccgc cgggaactgg aaatcgaaga accggcgatt cttgaagcca
     6841 tcacttatca tatcaccggt gctgccggga ttggacctct ggcccggttg atctttgtcg
     6901 gtgattttat cgaaccgggt cgtacttttg aacaagcccg aattttacgg gatgaagcag
     6961 ataagctcgc tcccgaacag ttgttactca tggtatacaa ccgatccatc acttacgtga
     7021 ttaatcgtaa ttatctactg catccccgga gtctggaggg acgaaacgaa ttaatacaca
     7081 aaggtatccg ttaaattcca tggaaccctt attggaattg attaaggaag cagcgcttga
     7141 gaaaaaggcg ctggaaccaa tgctcctcga tctgcgaggg atctcgggaa ttaccgatta
     7201 tttttttatt tgttccggta atacacaagt tcagatccgg gcgattgccg atgcgatcac
     7261 cgacaaactg gaggcggcgg atatcccttt gccgcccaag gaaggatatc gtgacggtcg
     7321 ctggatcttg ctcgactgcg gaagcttggt tgttcatatc ctgaatcaac aggagcggga
     7381 gttttatgca ttggaaaatc tctggcatga cgccaaagtg attccggtac aataaacaaa
     7441 atgcacaaat gtgtagcgtc caactggcgc caataataga caacaggcaa taggcaaaag
     7501 ggttaaggca gtctggttac tggcctttgc cggatggaag tggaggacca attcaatggg
     7561 taaagtcgat aaataccatc atcgcgagat cgagccgcgt tggcaaaaca aatgggaaga
     7621 gaccggatta taccattcgg atattgcgga ccggccaaaa tattatgcta tgaccatgtt
     7681 gccgtatccc agcggtgatt tgcacatcgg acattggtac gccatgactc cttcggatgc
     7741 tcatgccagg tttaaaagga tgcagggtta taacgtgatg tttcccatgg gttttgatgc
     7801 cttcggctta ccggcggaga atgccgctat caaacgaggg gttcatccgt ataaatggac
     7861 tatgtccaat attgagcgga tgcgtcaaca gttgaaatct atgggagcgc tctttgattg
     7921 gcggcgtgaa atcgtttctt gtctacccgg ttattatcgt tggactcaat ggttttttct
     7981 caaattttat gaaaaaggca ttgcctataa aaaattcgca ccggtcgatt tttgccctca
     8041 gtgtaatacc actctcgctc gagaacaagt ttggggtgaa gatcgtcatt gtgaacgatg
     8101 cgaaactccg gtggtaaaac gggagttaaa tcaatggttc tttaagatca ccgattatgc
     8161 cgaagaattg cttgactttt cgggaatgga ttggccggaa cgggtcgtta cgatgcagca
     8221 aaactggatc ggccgcagtg aaggtgcaga agtggttttc cataccgaaa aaggtcaaga
     8281 aatgcgaatt ttcacgaccc gccccgatac cttatggggt gtgaccttta tggtattagc
     8341 ccccgaacat ccactggtcg atgagttaac gacttccgaa tatcgtgctc aagtggaaca
     8401 atataaacat caggctagcc gtcagagtga gatcgaacgt ttggcggtta caaaagaaaa
     8461 aaccggtgtt tttaccggag cttatgcaat aaatccggtg aatgggtcta aagtccccat
     8521 ctggaccgcg gattacgtta tgatgagtta cgggaccggt gccattatgg ctgtccccgc
     8581 ccatgatgaa agggactttg cctttgctaa gaagtaccag ttaccgattc gaatggtcat
     8641 cgcgccttcg gggataaatc tggatcaccc ccttccggaa gcatatacca gtgttgagga
     8701 agggctattg gtcaattcag cgcagtttga cgggactccg gccaaggaag ccaaacaaca
     8761 ggttacaaaa tggttagagg aaaaaggatt tggtaaaaag gctgtaaatt accgtctccg
     8821 cgattggttg atcagtcggc aacgttattg gggagcacca attcccatta tctattgcga
     8881 aaaatgtggt actgtacccg tgccgtacga agatttgccg gtacttctac ccgaggatgc
     8941 cgagttcctg ccgacagggg aatcaccgtt aaagtatcat gagggattta gaaagaccac
     9001 ttgtccgcga tgcggcggtc cggcggaacg ggaaaccgat accatggata cctttatgtg
     9061 ttcatcttgg taccaatatg gttatcttag cccttattac aaaggagacg tcccctttga
     9121 tcccaaagaa gccgcttatt ggcttccggt ggatctatat accggcggtg tggaacacgc
     9181 ctgcatgcat ttgatctata cccgtttctt caccaaagcc atgcgcgata tcggactggt
     9241 tgatttcgat gaaccgatgt tgaaacttcg gaatcaagga atcatcctgg gtgaagacag
     9301 cgagaaaatg agcaaatccc ggggaaatgt ggtagccccg gatgatttgg tccaaaaata
     9361 cggtgcagat gccgttcggg cttatctgat gttcggttgg cgctgggaac aaggcggccc
     9421 ttgggatagc cggggaatcg atggtatgta ccgttttatg aaccgcgttt gggaattgac
     9481 gttggaagag atgaagccgc tttcggccga accggcagat gccgcaaaat ccctggttcg
     9541 aaagactcat cagaccattc tcaaagcgac tcgaagtatt gaggattttt ccttcaatac
     9601 ctatttggcc tcactgatgg aattctctaa tgtaatggcc aagtataaaa cggaactata
     9661 tgcttcggat gcttggaatg aggcggttaa gagcctgctt ttgctgttgg ctccggcatg
     9721 cccccatatt actgaagaaa tatggtcccg actcggttac ggttattcca tccataacca
     9781 gccttggcca aaatacaatc cggaacttgc agccgaagag acagtggaga tcgtgcttca
     9841 gattaacggt aagatccgtg aaaagcttaa tgttccagta aatagtagtg aagaggatct
     9901 gaaagatctg gccttgaaga atcaggcagt agaaaaggcc attgccggca agactgttaa
     9961 aaaagtgatc gtggttccca accggttgat caacgtcgta gtcgcttaaa acatttttcg
    10021 tgaccatttc tctactctaa tttatcctcc ccggaaacgg ggatttttct tgtccgggga
    10081 tctttatact aagctgtttt taaaaaattg tgaggaaaaa tggaagaaca gatacgtctt
    10141 ttttggatcc ccatcttttt acaacccgag gaacgtcgga cggttaaggc caccttttcc
    10201 tcgggaaact gcgtgatcca atttccccgg cagatgttgg gccggaaaac ccagctgtta
    10261 acgacactgg atcatgtgta ctggcggctc atgggggagc ttgctttacc tgatctcaag
    10321 cagcgtaccg cagaattgaa tcaggcattt tttaagtttc attaccgcag cgttcgatat
    10381 catcgtcaat tccgccgttg gggctcttgt tccgaccggg gcaacattaa tctatcccac
    10441 cgcctcatcg gtgggcctcg tgaactcgtg gactacgtga tccttcatga attggctcac
    10501 ttgcaatact taaaccatgg acccgatttc tggagactcg tcaggagtac cggcgaggaa
    10561 cctcaacaga tccggaaggc catgacagtc tatggccggg aatggcagta ccactacgaa
    10621 aattggtacc gccggcttca tcagattatc caatctccaa aaccaaaaaa gtaagcccgg
    10681 ggaaactccg ggcttttttg tttgaataaa accccagtta ggcggggagt ttatccatta
    10741 taaaatggcc tggagcgctc agagttttac ctttgatggc cgggatcgag agccagctaa
    10801 ctgtgtataa atgaacggga tgtgaattga ttgtgcgctt tgaaaaacaa gcccctgcag
    10861 tttatcctgt ttttcgcgct ttttctgctt ggatcttcag taatcgcggg actaattgaa
    10921 tatatgaatc ttcccggaac gcttcatctt tctccggggc aaaagattac cctttccgtt
    10981 cattacccgc tgagtttcca ttcgggaagt tccttgacat ttcaggaatc gttgaaactt
    11041 tcagcattcg atcatctgct cacgaaggaa ttggtattat ctccaaaatc cgcagaacga
    11101 tttgaggttc aaatccggat cttcggcaga attccggtta aaaaactcca ggtggaagtg
    11161 gctgaaccgc cccaactcat tccaggaggg caggccgtag gggtgctttt ttccagcaaa
    11221 ggggtggtga tcgtaggcca tttgccgatt aagggagtcg acggcaagat gtattaccct
    11281 gccaagacag ccggattaaa ggtgggtgat gtcttgcttg ccatcaacag cctcccggtg
    11341 aatcgagtgg atgatgtgga agtgttgttg cgtaattttc gtccctggta ccggacactc
    11401 aacctgacca ttaagcgagg aaatcagatc cggttaatca aggtacatcc ggtcttaagc
    11461 tatgaggaag gaacccaaca ccagcgttat atgctgggaa tatttatcga agaccccgca
    11521 gccggcgtgg gaacgttaac tttttacgac cccgttaccc ggaagtttgc gggtttaggc
    11581 catcatatcg ctgagtttag cggcgcgaaa ggtttgacct tgcaaaacgg agaaattgtg
    11641 ctggcatcga tcaatggcat taaagcagga cttcccggac aaccgggtga aaagatcgga
    11701 gtttgcagta ccaacttgag tccgatcggc ctcatcgaaa agaacagtcg tttcggaata
    11761 tacggaaccc tcaccgaaaa ctttatcgat tttaccaaag caagatcctt gcccatggcg
    11821 tatagttctc agatcacctt gggctccgcc gagatctata cggttatcaa ggagcggttg
    11881 gttgaaaagt ttcaggtcca gatcataaag gtgtaccggc aggattctcc acgcgacaaa
    11941 ggtttaatct tgaaagtaac cgatccggaa ctgctcaaac gaaccggggg gattattcag
    12001 gggatgagcg gcagtcccat tatacagaac gggcggttaa tcggggctgt gacccatgtt
    12061 ttcg
//
LOCUS       DASVMB010000521         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_577558,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000521 DASVMB010000000
VERSION     DASVMB010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577558"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VF531_14690"
     CDS             complement(<1..360)
                     /locus_tag="VF531_14690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7715261.1"
                     /translation="MRIGIFTNCYLPLVNGVVGAVSLLRRGFMEAGHQVYIFAPDFDD
                     YVDQEPDIYRYPAVDLTRRVKYPVAIPFAPRMHRVLPELNLDIIHCHHPFVLGIEGLK
                     AARRLGIPSVYTFHTQYD"
     gene            complement(616..984)
                     /locus_tag="VF531_14695"
     CDS             complement(616..984)
                     /locus_tag="VF531_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HEX7715262.1"
                     /translation="MLIPIICAVIGVLVGIFLPFDIPINFVQYTAIAILTALDAIFGG
                     IRAQLERKFNFYRFISSFFTNAALAALLAYLGNLLGVDIYMGAVVAFSIRLFQNLSII
                     REELFDRFWWSHANGRSKDV"
     gene            complement(984..1691)
                     /locus_tag="VF531_14700"
     CDS             complement(984..1691)
                     /locus_tag="VF531_14700"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX7715263.1"
                     /translation="MYKVKSWQTGIGLVAMVLSIIVVLQLRTELKIGATLPTRRVGEL
                     SQMFKNQSLQLKKYELEISDLRNQLNNYDRDREITRLKMAAGLVPLAGKGLRITLSDS
                     EKKLKDYEDPTFYIVHYSTLELLVNELWAAGAEAVAVNGYRVGNTSGFSCAGTTILID
                     TKRLAPPYLIDVIGDPRNLKNALMMRGGFVDTEILAFDLKFEIQEENEETITVPAYKG
                     SISFEYAKPAEEEEAKK"
     gene            complement(2047..>2657)
                     /locus_tag="VF531_14705"
     CDS             complement(2047..>2657)
                     /locus_tag="VF531_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7715264.1"
                     /translation="GAGLPVHEATGNMVIDVGGGTTDIAVLSLGGIVTGQSVPVAGDS
                     MDEAIARYIRRTYNLMVGERTAEDIKIYLGSAFPMEKEIEYEVRGRDYVTGLPKVIRV
                     SSEEIRNSLKEPLNTIVNAVRIIIEKTPPELVADIMFKDIVMTGGGSLLKGFDKLLGS
                     ELGMNVRVVEDPLTSVAIGTGKTLEHIHLLSRVVAAAKKASS"
BASE COUNT          721 a          701 c          574 g          661 t
ORIGIN      
        1 atcatattgg gtatggaagg tataaaccga tggtataccc aatcttcgag cggctttcag
       61 gccttcaatt cccaacacga agggatgatg gcagtgaata atatccagat tcagctctgg
      121 taaaacccgg tgcattctcg gcgcgaatgg gatcgccacc gggtacttta cccgccgggt
      181 caaatctacc gcagggtacc ggtagatgtc gggttcctga tcgacgtaat catcaaaatc
      241 cggagcgaat atgtagactt ggtggcccgc ttccataaac ccccggcgca ataaactcac
      301 ggctccgacc accccattga ccagagggag ataacagttg gtaaaaattc cgatgcgcaa
      361 gatctcttgc ctcgctttac attgtatggt ttttgccgat taacggtatg atttctatcc
      421 cgcccaatgt ctaccttatt cgcgatttac tctccaaatc ctgccatata attcggtctc
      481 gagccatggc tgactaccgg tgatgaaaat caaaaaagta ccccaggggt acttttagcc
      541 aatttgcaaa cgaaattggt tcaaaatcct aagatgcctt ttattcaatt gaaagtaact
      601 tcgttcgtaa gtgcattaaa cgtctttact gcggccattg gcatgggacc accaaaagcg
      661 atcgaataac tcttcccgaa tgattgagag attttgaaag agccggatgc taaaggctac
      721 cactgcaccc atatagatat ccacgccaag aaggttgccc aaatatgcca aaagtgcggc
      781 gagggcggca tttgtaaaga aactgctgat aaaacggtaa aagttgaatt tccgctccaa
      841 ttgcgcccgg ataccgccaa aaatggcatc taatgcggtc aagatggcga tagcggtata
      901 ttgcacaaaa tttatgggaa tgtcaaaggg tagaaaaatg cctactaaga cacctattac
      961 cgcgcaaata atcgggataa gcattacttc ttggcctcct cctcttcggc gggtttggcg
     1021 tattcaaacg aaatgctgcc tttataagcc ggaacagtaa ttgtttcctc gttttcttcc
     1081 tggatctcaa atttcaaatc aaacgccagg atctccgtat caacaaaacc gccccgcatc
     1141 atcaatgcat tttttaaatt gcggggatcc ccgattacat cgatcaagta aggcggcgcc
     1201 aaccgtttcg tatcgatcaa gatggtggtg ccggcgcaac taaaaccgga ggtgttcccc
     1261 acccgataac cattgacggc gaccgcctcg gctccagcgg cccataactc gttgaccagc
     1321 aactccaatg tgctgtaatg gacgatataa aaggtgggat cctcatagtc tttaagcttt
     1381 ttttccgaat cactcagggt gattcgcaat ccctttcctg ccaaaggaac cagtccggca
     1441 gccatcttca accgcgtaat ctctcgatcc cggtcataat tgttcagctg gtttcggagg
     1501 tcgctgatct ccagttcata cttttttaac tgcagggatt gattcttgaa catctgcgag
     1561 agttcaccga cgcggcgggt cggcaatgta gcgccgatct tcaactcggt gcgcagctgc
     1621 aaaacgacaa tgatgcttaa aaccattgcc accaagccaa ttcctgtttg ccagctcttt
     1681 accttgtaca aacgaaacgc ctccattatt ttacgcgcag gcgcggggca aagcccttga
     1741 gcccgagccg ggaagaaacg ccgccgacgg tagatcaaac aagggatcaa gtcagctaat
     1801 gataatcata atccaaaact ccgacaaatg caacttccat tcatagcctc cggtctgaaa
     1861 aaaagagtta accggagaga tacccgtccg gaggaaaaaa ggtcaacaaa atagcactgg
     1921 atatcaaccc aatgatgatt gtaccagtgc aacattaata ctaagttgct ttcaattgaa
     1981 caatttatca aaaagtaact tggtatttta aggaatcaac tcgtttggcc tacaatgaat
     2041 ccatctctag ctgctggcct ttttagcggc agctacgacc ctggataaaa gatggatatg
     2101 ttccagtgtc tttccagtgc caatggcgac actcgtcaag ggatcttcaa ccacccggac
     2161 attcatcccc agttcgcttc cgagtagttt gtcgaaccct tttagaaggg agccgcctcc
     2221 cgtcatcacg atatctttaa acatgatgtc cgccacaagt tccggcggag tcttttcgat
     2281 aattatacga accgcgttga cgattgtgtt caacggctcc ttcaatgaat tgcggatctc
     2341 ctccgaacta acccgaatca ccttggggag cccggtgaca taatccctcc cccggacctc
     2401 atattcaatc tctttttcca tcgggaatgc cgagcccaga tagatcttga tatcttccgc
     2461 cgtccgctct cccaccatca aattatacgt acgccgtata tagcgggcga tggcttcatc
     2521 cattgaatct ccagccacag gaactgattg acctgtaaca atgcctccca gggaaagcac
     2581 tgcgatgtcc gtagtgcctc cgcctacatc gataaccatg ttcccggtcg cctcatgtac
     2641 cggcagacct gcaccga
//
LOCUS       DASVMB010000522         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_139546,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000522 DASVMB010000000
VERSION     DASVMB010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139546"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF531_14710"
     CDS             complement(<1..369)
                     /locus_tag="VF531_14710"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7715265.1"
                     /translation="MRIIRFFDVFFAFYAVKGMTVEMADTPKLIITASAIFHMPDLLR
                     ELHLTGTLLLIADTNTYRVAGSTVASVLKSGGWQLTECIFERSGVLVPDERALGEILI
                     KLEPDTQGIVAVGSGVINDLA"
     gene            complement(400..1092)
                     /locus_tag="VF531_14715"
     CDS             complement(400..1092)
                     /locus_tag="VF531_14715"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX7715266.1"
                     /translation="MPYQLVATDIDGTITDPSGRVHLKALEVIRELEEKGIPVILVSG
                     RPLPYVESLSLYMGTSGPLIAENGAVLKFGRQLILAGEAELAKKALALLERDFSRAGA
                     RLTLDEDNRYRMVDVTLEKTVDPELIRHRLDQAGLPVTLLVTNVMLHLVDAKVSKGKA
                     VLQALEFLGIAPSRAVVCGDSFNDLPLFAIGALNLAVGSAEPSLKQKADRVASLPGGA
                     GFAELVRETFGL"
     gene            1369..>2253
                     /locus_tag="VF531_14720"
     CDS             1369..>2253
                     /locus_tag="VF531_14720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715267.1"
                     /translation="MPYYRRNLIVLSITIFMAATSWNQILPFLPLFLKELGVKSGIAF
                     WSGMLLSVQAITVAFSGPFWGKMADKYSRKLMTIRAGFCLALIYFGMSLCQSLWQLVL
                     LRFLNGLLTGFIPGSLALIATNTPKDEAGRYLSIAQTANAIGGIAGPVVGGILAGVFG
                     YRFSMAISGVAILIAMLLVLFLVEERYRAEITQKTSIWRDYQLAIQMPTLQTVILAEV
                     AGAVMTGAVLPIIPLYLEQIAQGSVTTISGIIFSLPGIAFVLTAYAWNRRGEKTTYPG
                     VILAGLGGACLMMLLQGFL"
BASE COUNT          550 a          600 c          536 g          567 t
ORIGIN      
        1 ggccagatca ttgataaccc ctgaaccaac cgccacaata ccctgggtat ccggttccag
       61 cttgatcagg atctctccca gcgctcgctc atccggaacc aatacgccgg aacgttcgaa
      121 gatgcattcc gttaactgcc aaccgccgga tttcaatacg cttgctacgg tagagccggc
      181 cacccgatag gtgttggtat cggctatcaa taggagggtg ccggtgagat gcaactcgcg
      241 aagcagatca ggcatatgga aaattgcgct ggctgtaatg attagcttgg gagtatccgc
      301 catttcaaca gtcattcctt tcactgcgta aaaagcaaag aaaacatcaa aaaacctaat
      361 tattctcatt tacaaggcac tcgtaacgaa taactgtttt tatagcccga aggtttcccg
      421 gaccaattcc gcaaatcccg cccctcccgg gaggctggcg acccgatccg ccttttgttt
      481 taaggaaggt tcggcgctgc cgacagccag attcaatgcg ccaatggcaa agaggggcag
      541 atcgttgaag gaatcgccgc aaaccacggc ccgggatggg gcaataccta aaaactccaa
      601 ggcctgtaat acggctttgc ccttggatac tttggcgtcg accagatgca gcatgacatt
      661 ggtaaccaga agagtaacgg gcaggccggc ctgatcgaga cgatggcgga tgagttccgg
      721 atctaccgtt ttctccagcg tcacatcgac catccgataa cggttgtcct catccaaggt
      781 tagccgcgca cccgcgcgtg aaaaatcccg ttccaggaga gccagtgcct tcttggcgag
      841 ttccgcttca cccgctaaaa ttaactgtct gccaaatttc aaaaccgctc cattctcggc
      901 gatcaaaggt ccgctggtcc ccatgtaaag ggaaagactc tcaacatacg gcagaggacg
      961 accggaaacg agaattacag ggatcccctt ttcctccaac tcacggataa cctccaacgc
     1021 tttcaaatgc actcttccgg atgggtcagt gatcgttccg tctatatcgg tggcaaccag
     1081 ttggtagggc atcaaaccga cctcgatttt agatttttaa caagtcttga tataaataat
     1141 cccatattaa ttcgtttttg attccgaaaa tccttgattt gcagttgctc ccaaggatgt
     1201 tcaaagatta tatataaaac aattgattgc ctcagccact ttaaatcccg tttcaagtcg
     1261 ttgaagggtg gaaaccaaat gttaacattc atgtaatatc aaaatccttt agaataagaa
     1321 tgagattctc gaaaacatct ccggaatgcc ggggaaagga ttgcagacat gccttactac
     1381 cggcgaaact tgatcgtgct ttccatcacg atcttcatgg ccgccaccag ctggaaccag
     1441 attctcccct ttctcccgtt atttctaaag gaactagggg tcaaaagcgg tattgctttt
     1501 tggtccggaa tgctcttatc ggtgcaggca attacagtgg ctttctccgg gcctttttgg
     1561 gggaagatgg ctgacaaata tagccgtaaa ctaatgacca tccgggccgg attctgtctg
     1621 gccttgatct atttcggaat gagtctctgc caatcccttt ggcagttagt actgctccgg
     1681 ttcttaaatg gtctgctcac cgggttcatc cccggctccc tggcgctgat cgccactaat
     1741 actcccaagg atgaggccgg gcgctatctc tccatcgccc agacagccaa tgccattggg
     1801 ggaatcgccg gacctgtcgt aggtgggatt ttagccggag tcttcggcta ccgtttttca
     1861 atggccatct cgggtgtggc gatcttaatc gccatgcttc ttgtgctgtt cctggtagag
     1921 gaacgttacc gggctgaaat cacccaaaaa acttccattt ggcgcgatta ccaactggct
     1981 attcaaatgc cgaccctaca gactgtaatc ctggccgaag tggccggcgc ggtcatgact
     2041 ggagccgttt taccaatcat cccgctctat cttgagcaga tcgctcaagg ttcggttaca
     2101 accattagcg gaatcatctt ttccctaccg gggattgcct ttgtattaac ggcttatgcc
     2161 tggaatcgcc gcggtgaaaa aaccacttac cccggagtca ttctggccgg tttggggggg
     2221 gcctgtctca tgatgttgct gcaagggttc ctt
//
LOCUS       DASVMB010000523         6843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_287269,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000523 DASVMB010000000
VERSION     DASVMB010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287269"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1712
                     /locus_tag="VF531_14725"
     CDS             <1..1712
                     /locus_tag="VF531_14725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715268.1"
                     /translation="FQQILEKEVSSSYEQVVAQYVGSIDNKLTLYRNLLDSITVNGMV
                     QDIFSRQDQFRINSTLDIGRKFSKEIDNQLFVKNSNEIYAIMLYALNRQFPSDGIHTS
                     NVEKVKNEAWYQEMIKRSDTTHYFSYIIKGLDLNVVSLVKPIPDLTGNRYLKKLGFAK
                     IDLVATSLFKMDNRRADRDNYDIYILDPAGTVLFTNSTKPFPFTGKKNLHEILGSDTG
                     RWTIKDSNHNGILIHHKIGGCGWTAFVMFQNDELENKVKEIGSFILFIVLLMLLILIC
                     LTALFTRIFTRRTLLLTDKMNRIEKGELTVGSVIDGKDEIGNIDRHFNRMILRLNDLI
                     NENYIQKIEKREAELKALQFQINPHFLYNTLESISAIASVYGCREICTICEKLGAMFR
                     YNINKDHSEFVPLIEEIQHIQNYLYIQKVRFGDKFTTFFDIPDELKDCKVLRFILQPL
                     VENALYHGFEGKRVQGCLEISAFVTDGSLIIMIYDDGQGMTAEQVERINTYINENDSR
                     SIGEYKESIGIKNVNSRIKLVCGEEYGITIKSTLNSGTQVRITLPFDHAKGDGNVQNP
                     DCR"
     gene            1687..3285
                     /locus_tag="VF531_14730"
     CDS             1687..3285
                     /locus_tag="VF531_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715269.1"
                     /translation="MYKILIVDDEKWIRKGIIAKLRHLGFEFSWIGESENGAEAVELI
                     REQTPQIVITDVRMPVMDGIQMIKNLREAGWDIKTIIISGYADFHYAEQALNLGVWGY
                     ILKPIAEGNLRETLEKVMARLDEEKELQTIKEHTKALERNQEFLMQERAVNCIFHTPE
                     RIVEDSAPINLYREVMGEKAYYRLILIHVDNSNYTEASFKYRDLDFMKSAIRNIVEES
                     GAAYHVFVMDNLKDPTQLMIFLSHLDQKELNTNCQSFVNDVYAKIEQSLGIAVTIAVS
                     AGEEGITSELYRQAKAALDMRMIERCSRIHYYENIKTQEKLSLPEHKLKLLQNLIELH
                     ELQNVYTVLNDIFLPENLAGMSQNYIRLLYHEIIGIVLRNSGNMDKDLTDAFHTELVL
                     GEMLDYFDDPAQIVMYLYTTIVDMYKEPPGVGAYCREMVSKAREFIINNYFQEITVKD
                     LACRFAINPNYFSTIFRQETGVTLTKFIMDVRMEKACGLLQETQASISEIARIVGYPD
                     VQYFYRVFKKATGHTPLEFRNSGN"
     gene            3450..3698
                     /locus_tag="VF531_14735"
     CDS             3450..3698
                     /locus_tag="VF531_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715270.1"
                     /translation="MSSSLPDWDFIQDLMANEAALGAFAVEPNPDEVQNLLDSIKQQG
                     MIACVKDDAVFFLGQEECRQQGIPFKHQIYSNTIAQAS"
     gene            complement(3814..5313)
                     /locus_tag="VF531_14740"
     CDS             complement(3814..5313)
                     /locus_tag="VF531_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX7715271.1"
                     /translation="MKGIMIQGTSSDAGKSFLVTALCRALANQGLKVCPFKSQNMSNN
                     CYVTPDGLEMGRAQAVQAEAARLKPDVYMNPILLKPCKDTLSEIVLLGKVHPTPGDSD
                     YFKNFTTTTGIQTVKNALRHIEAHYDAIVIEGAGSPAEINLNSHEIVNMRIAREADVP
                     VILVTDVDRGGSLASVVGTLELLGEDRNRVKGIIFNKFRGDMALFQDAVRWTEDYTKT
                     KVIGVMPYLHDILVESEDSLSIDNHYKSLQANKIVIGVVKMPYVSNNTDIEPFRFEED
                     VEIILIDAFTDFTKLDAVIIPGTKSTISDMKYLHDSGIAKALKTFHDNGGFIFGICGG
                     YQMLGEKIVDELGVDNPSVKNIEGLRLLPVRTTFTAKKTVNQVSGRLANGKLLAPGLS
                     NCEVSGYEIHFGITERMGDIGHPLFLLNNHQDGMEVDNLRIAGTYLHNVFHNDAFRSL
                     WLNRIRAKKQYAEKPPVNTEKIKEQSYEALAKAASEYLDMEYVLKLVNN"
     gene            5670..6383
                     /locus_tag="VF531_14745"
     CDS             5670..6383
                     /locus_tag="VF531_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715272.1"
                     /translation="MNVGKSIQQSIDVYLKNFGILISACLITGLVSVFSVGILAGPLV
                     GGVLILGLKILRGEPGKLTEIFAHFDQLVPTLVATLILWGVGLIFWIIGIIPVIGMVI
                     SFVATPVLSLLYFLVIGFIVDQKMRPLEAVKRSCDYFAAEPLQLWLYSLAMMILGGIG
                     AIIFLVGVFLTMPLGMMGMIIDYQRLSTKETPLFEPGKQTLRIAGTALAVLFITGVIC
                     WALGIERISYPAFLFQSAR"
     gene            6416..>6843
                     /locus_tag="VF531_14750"
     CDS             6416..>6843
                     /locus_tag="VF531_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715273.1"
                     /translation="MLRKWMIVLCFGSLMLLAAVTGYTADDEEYDTDAVMQQAIANKD
                     KFPPEIVALVQPKVTVTNHTYSADPISRSFVWIEFYGEKPVEAYPGGIFQETSYYFKL
                     AAYDSNSDMGKFYIQAEQNNARPEAYRNARQLMKDLSFND"
BASE COUNT         1864 a         1485 c         1622 g         1872 t
ORIGIN      
        1 aatttcagca aatccttgaa aaagaggtca gttcctctta cgaacaggtt gtcgcccagt
       61 atgtcggcag tatcgacaac aaattaactc tctaccgcaa cttgctggat tcgatcacgg
      121 tcaacggcat ggtacaggat attttttcac ggcaggacca gtttcggatc aatagcaccc
      181 tcgatatcgg caggaaattt tccaaagaga tcgataatca gctttttgtc aaaaatagca
      241 atgagattta tgccatcatg ttatacgcgc tcaaccgtca atttccgagt gacgggatcc
      301 ataccagcaa tgtggaaaag gttaaaaacg aagcttggta ccaagaaatg atcaagcggt
      361 cggatactac ccattacttt tcctatatca tcaaagggtt ggatctgaat gtggtctcgc
      421 tcgtaaaacc cattcccgat cttaccggga atcgatatct gaagaaattg ggatttgcca
      481 aaatcgattt agtcgcgacc agcttgttca aaatggacaa ccgtagagcc gaccgggaca
      541 attacgatat ttatattctc gacccggcgg gaaccgtatt atttacaaac agtacgaagc
      601 cattcccctt cacgggaaag aaaaacctcc atgaaattct gggaagcgac accggacggt
      661 ggacgatcaa ggattcaaac cataacggga ttctaatcca ccacaaaatc ggagggtgtg
      721 gttggacagc ttttgtcatg ttccagaacg acgaactgga aaataaagtc aaggagatcg
      781 gttcatttat tcttttcatc gttctgctga tgctgttgat attgatctgc ttgacagctt
      841 tgtttaccag gatctttacg cgcaggacct tattgctgac ggataaaatg aaccgaattg
      901 aaaagggcga acttacggtt gggagcgtca tcgacgggaa ggacgaaatc ggcaatatcg
      961 accggcactt taaccggatg atcctcaggt tgaacgattt gatcaacgaa aactacattc
     1021 aaaagattga aaaacgggaa gcggagctta aggcactgca gtttcagatc aaccctcatt
     1081 ttttgtacaa tacgcttgaa tcaatcagtg caatcgcttc ggtatatggt tgccgtgaaa
     1141 tctgtaccat ctgtgaaaaa cttggagcga tgttccgtta caacattaac aaggaccatt
     1201 ccgaatttgt cccgttgatc gaggagatcc aacacatcca gaattacttg tatattcaaa
     1261 aggtccgatt tggggacaaa ttcacgacct ttttcgatat cccggatgaa ctcaaagact
     1321 gtaaagtatt aaggttcatt ctgcagcctc tggttgaaaa tgcgctttac catggtttcg
     1381 aaggcaagcg agtacaggga tgccttgaaa tatcggcttt tgtcacagac ggatctttga
     1441 tcatcatgat ttatgacgac gggcagggga tgactgcaga acaggtggag cggatcaata
     1501 catatatcaa tgagaatgac agtcgttcca tcggtgaata taaggagagt atcggcatca
     1561 agaatgtcaa ttcccgaatc aagcttgttt gcggtgaaga atacgggatc actattaaaa
     1621 gcacattgaa ctccggtacc caggtgagga tcacgcttcc tttcgatcat gctaaggggg
     1681 atggcaatgt acaaaatcct gattgtcgat gatgaaaagt ggatccgtaa ggggatcatc
     1741 gccaaacttc gccacctcgg ctttgaattt tcatggatcg gagaatcgga gaacggggcg
     1801 gaggcagtgg aattgatccg ggaacaaact ccgcagattg tgatcacgga tgtgagaatg
     1861 ccggtgatgg atgggatcca gatgatcaag aacttgcgtg aggccggctg ggatataaag
     1921 accatcatca tcagcggtta tgcggacttc cattacgcag aacaggcgct taacctgggt
     1981 gtgtggggat atatattgaa accgatcgcg gaaggcaatc tccgggaaac tctggaaaag
     2041 gtaatggcaa ggcttgatga ggagaaggaa ttgcaaacaa taaaggagca tacgaaggct
     2101 ctggaaagaa atcaagagtt tctgatgcag gaacgcgctg taaattgcat ttttcacact
     2161 ccggagagga ttgttgagga ttctgcgccc atcaaccttt accgggaggt aatgggtgag
     2221 aaagcgtact ataggctgat tttgattcat gtcgacaatt ccaactacac agaggcatcc
     2281 tttaaatata gggacctgga tttcatgaaa agcgccatca gaaacattgt ggaagagagc
     2341 ggcgccgctt accatgtttt cgtcatggat aacttgaagg atcccactca gctcatgata
     2401 tttctttccc atctggatca aaaagaattg aacacgaatt gccaatcatt tgtaaacgat
     2461 gtgtacgcca aaatcgaaca atccctgggg attgccgtaa caatcgccgt aagcgcaggt
     2521 gaggaaggta taacatccga gctttatcga caggcaaaag ccgctcttga tatgcgaatg
     2581 atcgaacgat gcagccgaat ccattattat gagaacatca aaactcaaga aaagttaagc
     2641 cttcccgagc ataaactgaa actgttgcag aatctcattg agttgcatga gcttcaaaac
     2701 gtatataccg ttttgaacga tattttttta cccgagaacc ttgccggaat gtcacaaaac
     2761 tatatccggc ttttgtatca tgagattatc gggattgtat tgagaaactc cggcaacatg
     2821 gataaagatc ttacggacgc tttccatacg gagctggtct taggcgagat gctcgactat
     2881 ttcgacgatc ccgcccagat tgtcatgtat ctctatacaa cgattgtaga tatgtacaaa
     2941 gaaccccccg gtgtaggcgc gtattgcagg gagatggtat caaaggccag agagtttatt
     3001 atcaacaatt atttccagga gatcaccgta aaggacctgg catgccggtt tgccatcaac
     3061 cctaactatt tttcgacgat cttcaggcag gaaaccggtg taaccctgac caaattcatt
     3121 atggatgtca gaatggagaa agcatgcggc cttttgcagg aaacgcaggc gtccattagt
     3181 gagattgccc ggattgtcgg atatccggat gtgcaatatt tctaccgggt ttttaagaag
     3241 gcaaccggtc atactcctct ggaattccgg aacagtggga attagggaag gtcctagatc
     3301 acaagtgaat cttgcgggga ggaaatgaaa ttgttacact tttcccttta tttaggacca
     3361 atgttatgtt aaaattcata aatgagagag attttatcga aagccgtata ccataaagta
     3421 acgaaattcc tttttaggag gctacaacat tgtctagttc gttgcctgac tgggatttca
     3481 ttcaagattt gatggccaac gaagccgctt tgggagcttt tgccgtggaa ccgaacccgg
     3541 atgaagtgca gaatcttttg gatagcatca agcaacaggg aatgatcgct tgtgtcaagg
     3601 atgatgcggt cttctttttg ggtcaggaag aatgccggca acaagggatc ccgttcaaac
     3661 accaaattta ctcaaacacc attgcccagg cctcgtaaac cttttgatgg aacatcgtaa
     3721 ttctatgtaa gccaacagta ttcccgaaat gaaattcagc cgtatctcct ttgatgtgag
     3781 aacggttttt attttccctt ttatcttttc tttttaatta ttcaccagct ttaacacata
     3841 ttccatatcc aaatattcag atgcggcctt tgccagtgct tcatagcttt gttctttgat
     3901 cttttccgta ttcaccggcg gtttctccgc atattgtttt ttggcccgga tccggtttaa
     3961 ccaaaggctg cggaaggcat cgttatggaa aacattatgc agatatgtgc cggcaatcct
     4021 caggttatcc acttccattc cgtcctgatg attgttgagt aaaaacaatg gatggccaat
     4081 atcccccatt ctttcggtga tgccgaaatg gatttcatag ccgctgactt cacagttgct
     4141 caatcccgga gctaataact tgccgttcgc taacctgccg cttacttggt tgacggtttt
     4201 cttggcggta aaggttgtcc tcaccggcag cagtcttaag ccttcgatat tcttgaccga
     4261 tggattgtcc actcccagtt cgtcaacgat cttttcaccc agcatctgat aaccgccgca
     4321 tattccgaaa atgaaaccgc cgttgtcgtg aaaggttttg agcgccttgg caatgcccga
     4381 gtcgtgcagg tatttcatgt ccgaaatggt acttttggtg ccgggtatga tgactgcatc
     4441 gagtttggta aaatcagtga aggcgtcgat gaggatgatc tcgacgtcct cttcaaaacg
     4501 gaagggctcg atgtcggtgt tatttgatac atagggcatt ttgacaacgc cgatgactat
     4561 tttgtttgcc tgtagacttt tgtagtggtt atcaatgcta agcgaatcct cactctcgac
     4621 caatatatca tgtaaatagg gcatgacccc gataaccttg gttttcgtat aatcttcggt
     4681 ccaccggacg gcgtcttgga agagggccat gtctccccgg aatttattga agatgatacc
     4741 tttcacccgg ttgcgatctt cgcccaataa ctccaatgtg ccgacgaccg aagcaaggga
     4801 accgccccgg tccacatcgg ttactaatat aaccggcaca tcggcctcac gggcaatccg
     4861 catgttgacg atttcatggc tgttcaggtt gatttccgca ggacttccgg cgccctcaat
     4921 cacaatcgca tcgtaatggg cttcgatatg gcgaagggca tttttaaccg tttgaatccc
     4981 ggtggtcgtg gtaaagtttt taaaatagtc gctgtcaccc ggcgtgggat gaaccttgcc
     5041 cagcagcacg atttcggata aggtgtcttt acatggcttt aagaggatcg ggttcatata
     5101 gacatccggc ttgagccggg ccgcttcggc ctgaactgcc tgagccctgc ccatttcaag
     5161 cccatccggg gtaacataac agttgttgga catattttga gatttgaagg gacatacctt
     5221 caatccctga ttggcaagcg ccctgcaaag agcggtgact aaaaagctct tgccggcatc
     5281 gctgctggta ccctgaatca tgattccttt catatcaagt cacccctaat gaatacttta
     5341 tcaatcaaca tcaacgcctg gtcaaattag cttcattttt caacgaaatc gaaattggtt
     5401 ccgaatgata cgggggacat cggaacgatc cggttttatc agatccattt aactgttgtt
     5461 ctaatattat acacccaagc ttgcatagcc tgtattcatc gtttttgttt agaatttgtt
     5521 gagagacggt tatttataat caagctgaat ttggtttgat atgaagccat tcacgaggag
     5581 gatggtaatg cccaaaaaat atcggccggt actgtcgatg gagattgccg gagcttttat
     5641 attcctggaa caactagggg gagcaacgaa tgaatgtcgg caaaagcatt caacaaagta
     5701 ttgatgtata tctcaagaat ttcgggatac tcatatcggc ttgtctgatc acaggtctcg
     5761 tctccgtatt ttcagtgggg attctggccg ggccgttggt cgggggcgtt ttgatcctgg
     5821 gactgaaaat actccggggt gaaccaggga aacttaccga aatttttgcc cacttcgacc
     5881 aactggttcc cactttggtt gcgaccctga tactctgggg agtcggctta attttttgga
     5941 tcatcggcat catcccggtt atcggtatgg ttatcagttt tgtcgctact ccggtattga
     6001 gtctgctgta ctttttagtt atcggtttca ttgtcgatca gaaaatgcgg ccgttagagg
     6061 cggtgaaacg cagttgtgat tactttgccg cagaaccgtt gcagctttgg ctttattctt
     6121 tggcaatgat gattttaggt ggaatcggag caattatctt cttggtcggc gtcttcctga
     6181 ccatgccttt gggaatgatg ggcatgatca tcgattatca gcggctttct accaaagaaa
     6241 cccctctgtt tgagccgggg aaacagacgc tcaggattgc cggtaccgcc ctggctgtgt
     6301 tattcattac cggggttatt tgttgggcac ttggaatcga acggatttct tatcctgcgt
     6361 ttttatttca gtccgcccgt taataggcaa gctagagaaa ggagataata aaaacatgtt
     6421 gcgaaaatgg atgatcgttc tatgcttcgg atcgttgatg ttactggcgg ccgtcaccgg
     6481 atataccgct gacgatgaag agtatgacac ggacgcagta atgcaacagg caatcgcgaa
     6541 taaagataag ttcccgccgg aaatcgttgc tctggttcaa ccgaaggtca ccgttaccaa
     6601 tcatacctat tcggcggatc caatcagccg ttcctttgta tggattgagt tctatggaga
     6661 aaaacctgtg gaagcttatc ccgggggaat ttttcaagaa accagttatt attttaaatt
     6721 agctgcttac gactccaaca gcgatatggg caaattctac atccaggccg agcagaacaa
     6781 cgccagaccg gaggcttata gaaacgcccg ccaattgatg aaagatttat catttaatga
     6841 tag
//
LOCUS       DASVMB010000524         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_725065,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000524 DASVMB010000000
VERSION     DASVMB010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725065"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..2053
                     /locus_tag="VF531_14755"
     CDS             497..2053
                     /locus_tag="VF531_14755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7715274.1"
                     /translation="MTFKKFNSRVINLWSKQVHRDKIFSISLAEIRLQLGVVNAILIG
                     LAVGLTGSKADYVVVFSYLFLNLVFLLIPVLRYMVTRPLRLLVLGMDLAVTGWMMART
                     GGAASPLYAFLFIPVLLSMLRFRYPGVIICCTGCALFLIAVTLGAGFYNYLILIPRIA
                     YLYLAGIVGSYLINHTYTVTEEVSKKLARWNIELQRLNNFSLEVTGSHDLDQIFNQTL
                     KTVLQSNPSQMAAIAMFNDQGLLKIFASTGWDEKWLDYYDEHPLNLNSFTLAPIIIFK
                     NPLLCSDIKKHTELVRVFSKIPVEALFAFPLVVDGEVVGVLMITSPKVRQMSDQETQI
                     LTSIANQAGIAVQNVVSLRQERKKADTDGLTGLFNRRYFNEQIEELTRQALAHQVRLS
                     LIMVDVDDFKKYNDTYGHPAGDQLLKEIAAAIADTTREQDIAARYGGEEFAVILYDTS
                     NQMALLIADRIRLAVARIPSNRLKCPVTISAGVATLPDHARDRATLVDRADRFLYQAK
                     NSGKNQVCCG"
     gene            2120..2779
                     /gene="pgmB"
                     /locus_tag="VF531_14760"
     CDS             2120..2779
                     /gene="pgmB"
                     /locus_tag="VF531_14760"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEX7715275.1"
                     /translation="MKLKAVIFDLDGVIVSTDECHYQAWKRLADSEEIYFDREINKRL
                     RGVSRMESLAIILERSDKVYPDDAKERMAQAKNDYYRKLIADLTPAQILPGVMAFLDD
                     LRKAGLKLAVGSSSKNTPLILDRIGLAHFFDAVADGNEITKSKPDPEVFLLAAQKLGI
                     APESCAVVEDAEAGIEAALAGGMLVLGVGAAAGSQRATFRAQDLSAIPAADFLDRCSG
                     L"
     gene            2808..>3949
                     /locus_tag="VF531_14765"
     CDS             2808..>3949
                     /locus_tag="VF531_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715276.1"
                     /translation="MRLWRYSLLVWLVAFPVAATVLAAPRVEIVLTTPSEDTLGAIRQ
                     LVQEFNSKQNAILVKHRLLSSASDACHNFYLTSFLARSDSFDVFSGDIIWTAEFASSG
                     WIEPLDSFFKKRKQAEFLPGTIESCTYDGKIWAVPWYTDFGVLFYRKDIVKQPPKTWA
                     QLIAMSKENIRNGKVRYGYVFQGNQYEGLVCNALEFIFNNGGQVLSGNRVTINSPEAV
                     EGLRTLVEIMKISPEDVISFQEEDSRLAFQEDQILFLRNWPYDWNLLNQNGSPVKGKV
                     GVAPLPVGPGGSVGGGSLGGWNLMINRFSKNKLAAWKFVEFLTGKEAQKVNSTMGGRL
                     PTRTAVYSDPEVLRKNPYYSDFLTAYSRSKPRPVSPYYPAISETMQ"
BASE COUNT          987 a          885 c         1044 g         1033 t
ORIGIN      
        1 ggttcggcgg agttcaaagc attaaagggc aagatcaacg gatataagga taaaataaaa
       61 ataaagaagt ctgggaaagg aaaaggacag aaaggatgaa cccgggatca ccgggttttt
      121 ttcttctcca aatatccaaa tatatttagt ttatgtggcc tgttcggcag ggaacagtag
      181 atagttggcg aactttttaa gatcttacct gtcgggtttg gggatacggt cgggagtcga
      241 caacatcgtt tggggtaaca gaaaatatcc aatttggaga acccctccat ccaccggtat
      301 cagggaaatc gattgattgc cttccttttc tggatataac agggaatgga gtatgatata
      361 atggttttag tttcaagacc gcataccgca gccttacctg attatgggtg gacaggctta
      421 tgatttaatc actttgatgc acatccgtat gaaacacact tacatccatg aactcgcgcg
      481 cgtgggcgcg taccgattga cgtttaaaaa gtttaatagc cgggtgatca atttgtggag
      541 caaacaggtt caccgggata agatttttag catttccctg gcagagattc gtctgcaact
      601 gggtgtggtg aatgctatct taatcggtct ggcggttggg ttaaccggtt ccaaggccga
      661 ttatgtggtg gtattttcgt atcttttttt gaacctggtt tttttactca ttccggttct
      721 ccggtacatg gttacccggc ccctccgtct actggtttta ggaatggatc tggcggtaac
      781 cggttggatg atggctcgga ccggtggtgc ggcgagtcct ctatacgcct ttttgttcat
      841 cccggttctg ctttcgatgt tacgtttcag atatccgggt gtcattatct gttgtaccgg
      901 gtgcgcccta tttctcatcg ccgtcacatt gggagccggg ttttataatt acctcatttt
      961 aattccacgg attgcttatc tctatttagc gggaatcgtc ggcagttatt tgattaatca
     1021 tacctatacg gtcacagagg aagtttctaa aaaactggcc cgttggaata tcgagctgca
     1081 acggctcaac aacttttctc tggaagtcac cgggagccac gatctggacc agatttttaa
     1141 ccagacctta aagacggtgc ttcaaagcaa tccctcccaa atggcggcga tcgccatgtt
     1201 taatgatcaa ggtttactga aaatcttcgc ttctaccgga tgggacgaga aatggctcga
     1261 ttattatgat gagcatcccc taaatctgaa cagctttacc ctggctccga tcattatctt
     1321 taaaaacccc ttgttatgtt cggatattaa aaagcatacc gaattagtca gggtatttag
     1381 caagatcccg gtagaggcct tgtttgcctt tcctttggtg gtcgacggtg aagtcgtcgg
     1441 tgtgctgatg attaccagcc ccaaggtacg tcagatgtcg gatcaagaga cacagatatt
     1501 aacgagtatc gcgaatcaag ccggaattgc cgtccaaaac gtcgtcagtc tgcggcagga
     1561 acgaaaaaag gcggatacag acggtctgac cggacttttt aaccgtcgct acttcaatga
     1621 gcagattgaa gaattaaccc ggcaggcatt agcccaccag gtaaggctgt cgctgatcat
     1681 ggtggatgtg gatgacttca aaaaatataa cgatacttac ggacacccgg caggtgatca
     1741 acttttaaag gagatcgctg cggccattgc cgataccaca cgggaacaag atattgccgc
     1801 ccggtacggc ggggaggaat ttgcggtaat cctctacgat accagcaatc agatggcgct
     1861 gctgattgcg gaccggatcc gcctggcagt cgccaggata ccgtccaacc ggctgaaatg
     1921 cccggtgacg atctcggcgg gagtggctac actacccgat catgcccggg accgggcaac
     1981 cctggtagat cgtgccgatc gtttcctata ccaggctaaa aatagcggca agaatcaagt
     2041 gtgttgcgga tagttcattc acgaacaatt agtttagtac cggcctcgcc gggacctgac
     2101 caaaaagggg aattgaaaga tgaaactaaa agcagtgatt tttgatctgg acggtgtcat
     2161 cgtttccact gatgaatgtc attatcaggc gtggaaacgt ctggcggata gtgaagagat
     2221 ctattttgat cgcgagatca ataaacggtt gcgtggagtc agccggatgg aaagtctggc
     2281 aatcatcttg gaacggtccg ataaggtgta cccggacgat gccaaagaac gaatggcaca
     2341 ggcaaagaat gattattacc ggaagttgat tgcggatctg actccagccc agattctccc
     2401 gggagtgatg gcatttctcg atgatcttcg caaagccggc ctcaaactgg cagtaggttc
     2461 gtcgagcaag aacacaccgc tcatactgga tagaatcgga ttggcgcatt tttttgacgc
     2521 ggtagctgat ggaaatgaaa tcaccaagag taagcccgat cccgaggttt ttctattggc
     2581 cgcccagaaa ttgggaatcg ctccggaaag ttgcgcggtg gtagaggatg ccgaggcggg
     2641 gatagaagcc gcactggccg gaggtatgct ggtattgggt gtgggggccg cagcgggaag
     2701 tcaaagagct acgtttcggg cccaggatct ctcggctatt ccggctgccg atttcctgga
     2761 ccggtgtagt ggattataat tcgaacaagt attggagggg ggttctcatg aggttgtggc
     2821 gctattcctt gctggtttgg cttgtggctt ttccggtagc tgctacggta ctagccgccc
     2881 cccgggtcga aattgtttta accactccct ctgaggatac actgggagcc atccggcaat
     2941 tggtacagga attcaactcc aagcaaaacg cgattttggt taagcatcgg ttattatcct
     3001 ccgcttccga tgcctgccat aatttctatc taacttcatt tttggcacgt tccgatagtt
     3061 tcgacgtgtt ctcgggggac atcatctgga ccgccgaatt cgcctcgtcc ggttggattg
     3121 agccgctaga ttcctttttc aaaaaaagga aacaggccga gtttttgccg ggaacgattg
     3181 agagttgcac ctatgatggg aagatctggg cggttccttg gtataccgat ttcggagtct
     3241 tgttttatcg taaggacatt gtcaaacaac cgcctaaaac ctgggcgcaa ttaatagcca
     3301 tgtcaaaaga gaatatccgg aatggcaaag tccgatacgg gtatgtcttt cagggaaatc
     3361 agtacgaagg gctggtctgt aacgcactcg agtttatctt taataacggc ggacaagtat
     3421 taagcgggaa ccgggttacc atcaactctc ccgaagcggt tgagggatta cggactctcg
     3481 tcgagataat gaagatctct ccggaagacg tcatctcctt tcaagaggaa gattcgcgcc
     3541 tggccttcca ggaggatcag attcttttct tgagaaactg gccttatgat tggaatttgt
     3601 tgaatcaaaa cggttcaccg gtaaaaggga aagtgggcgt cgcgcctcta ccggtcggcc
     3661 ccggaggttc cgtaggcggt ggaagtctgg gcgggtggaa tcttatgatc aatcgttttt
     3721 ctaaaaacaa actggccgcc tggaagtttg tggagttttt gaccggcaag gaagcacaga
     3781 aagtgaactc caccatgggc ggccgtttac caacccgcac cgcagtttac agtgatccgg
     3841 aggttctgag gaagaatcct tattattccg actttttgac ggcttactcc cgttccaaac
     3901 cacggccggt ttcgccatat tatcccgcaa tctccgaaac catgcagat
//
LOCUS       DASVMB010000525         8049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_62954,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000525 DASVMB010000000
VERSION     DASVMB010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62954"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VF531_14770"
     CDS             <1..456
                     /locus_tag="VF531_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7715277.1"
                     /translation="IGKAVNHEIEGPHVTIGADLAKKYRESFQVVNAIAAHHGDVEAN
                     TVEAVLVQAADAVSAARPGARRETLETYIKRLEKLENIADSFEGVEKAYAIQAGREIR
                     IMVKPDKIDDLNSVRIAREIVKKIEEELEYPGQIKVTVIRETRAVEYAK"
     gene            509..1045
                     /locus_tag="VF531_14775"
     CDS             509..1045
                     /locus_tag="VF531_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715278.1"
                     /translation="MKGIFWKEGESFSNSMSLIAFLLVRYPEIGSVRFDPVQKTLQFS
                     FIVGKPLTEEEFQSFKDQLLLSLNNIAQLQGRDCGEIRIGFNVCDDLTFLEVIRDLES
                     LNQEEIALIIGVVKQGFEGSLLLDQEEGVQEEDEVIQEEMIEHMLEDLKDSHPEKRLI
                     GFREEGRVLVFNRANLHK"
     gene            1165..1950
                     /locus_tag="VF531_14780"
     CDS             1165..1950
                     /locus_tag="VF531_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX7715279.1"
                     /translation="MKVLFIGDVIGRPGRRILRTVLNEIIREEAVDLVIANGENSAGG
                     FGLTVEVCQELLGSGVDVITMGNHTWDNREIFKVLETEPRVVRPANYPGGTPGEGYYL
                     ARTGKGDLVGVVNLLGQVFLEPLACPFQTADECVAALKEKTPVIIVDIHAEATSEKMA
                     LGWYLDGKVTAVIGTHTHIPTADGRILPQGTGYITDAGMTGPVNSVLGIDPELVIKKF
                     LTKRPVRFEIASGPLQLDGVLLDIDTAGKTTRIQSIHRKLEVS"
     gene            2085..2621
                     /locus_tag="VF531_14785"
     CDS             2085..2621
                     /locus_tag="VF531_14785"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7715280.1"
                     /translation="MELVGTKIRLRPFAVDDLSKMAFWNKDEELQVYVDCDLPGNRDE
                     LEIWYHDNIPDRYYRIFAIETLKGQLIGDLELDHICWIKREAELRIRIGEKGYWGQGI
                     GAEALRLILEHVFTVRQFRRVYLRVYHFNERAIRCYSKLGFRPIGRLHRNTGNWKDII
                     LMELSNREYQRGLRSLAG"
     gene            2798..3601
                     /gene="spo0A"
                     /locus_tag="VF531_14790"
     CDS             2798..3601
                     /gene="spo0A"
                     /locus_tag="VF531_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HEX7715281.1"
                     /translation="MPKIKIMIADNNRELCDTLTDLITLQDDMELVGVAYDGIEALQV
                     IGQVKPDVLILDITMPYLDGIGVLERLSGFPEQPMVIVLTAFEQEAMIQRLIALGATY
                     YMVKPFDAPTLFERIRQFAAGKGSVPRRSNKAQEEQYIYAGTNRHEKSVNQLELEVTR
                     LFHEMGIPAHFRGYAYLREAIIIAAQEVEVLGNITKNLYPRIAEKFRSTTSGVESAIR
                     HTIEIGWERGNSEYMDEFFGFENQKGRFPTTASFIAKVADKLRLESKVV"
     gene            3876..4910
                     /gene="rlmN"
                     /locus_tag="VF531_14795"
     CDS             3876..4910
                     /gene="rlmN"
                     /locus_tag="VF531_14795"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7715282.1"
                     /translation="MLIKGLTREELDECLQRFALPRYRVDQIWSWLYQKAAVSWDEMT
                     NLPLSLRQDLAAAGFTLSGLHVVDKVTASDGTIKYLFRLEDDTTIESVFLPETDRNTV
                     CLSTQAGCGMGCAFCATGQNGLTRNLTAGEIIDQPLYIGRLQQTHLSNLVMMGQGEPF
                     SNYDAVLKAVRLINDPAGMGMGARHITISTCGVVPGILKLADEPYQVNLAISLHAVND
                     ELRSRLMPINRRYPLAELLESCRYYVAKTNRRITFEYTMIAGVNDGQADLKGLLAALR
                     GMLCHVNLIPLNPVPGSEWERSGKARIREFEAALNQAGIETTVRKERGSSLAAACGQL
                     QGKRGHDSRN"
     gene            4935..5507
                     /locus_tag="VF531_14800"
     CDS             4935..5507
                     /locus_tag="VF531_14800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7715283.1"
                     /translation="MRLLQFIGGQIQNIASLIHNRLLRLKHPQTKGTADSSRVKEGLD
                     QTRLRQETGSILQRMPSPVFRTILRLGRTEPNCLTLRRQRKRTLIQPVQTRWLFTVVS
                     GVDYGRQYMGITREIRVGRQPENHIQLRDPKVSRLHAVIHCRGAVLILEDHHSTNGTW
                     INGRRIFRKRLYPGDLIRVGETEIRVVQQK"
     gene            5534..6226
                     /locus_tag="VF531_14805"
     CDS             5534..6226
                     /locus_tag="VF531_14805"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX7715284.1"
                     /translation="MRVGAKTDIGKVREVNEDSYGYHGRLFVLADGMGGHEAGEIASA
                     IAVETVLAADLGDAPTTRLHQAILDANRAILAEVEKHPEHAGMGTTINVLYLDITQAY
                     ITNVGDSRIYRLSGGRLKQLTADDSLVAQLVKNGSITEEEAKVHPRPNVLIQALGMEG
                     NLEFEVTTVGIAPGDKFLLCSDGLTGMLDEKTITSYLDADMAPQSIAEQLVGAADEAG
                     GRDNITVIVVEV"
     gene            6425..>8049
                     /gene="pknB"
                     /locus_tag="VF531_14810"
     CDS             6425..>8049
                     /gene="pknB"
                     /locus_tag="VF531_14810"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX7715285.1"
                     /translation="MVGKVLGNRYRLLELIGEGGMAQVYKAEDSILCRTVAIKILRSQ
                     YAGDAEFVERFRREARAAASLSHPNIVNIYDVGQENGTDYIVMEYLPGCTLKQVIKDE
                     APLSVERALDITRQIAEGLNHAHQRNIVHRDIKPHNILITPDGQIKVTDFGIARAISA
                     GTLTQIGEVVGSVQYSSPEQAKGEAAGPQSDLYSLGCVLYELLTGTVPFKGDTLVSIA
                     LQHLQEEVVPIRQIRPDLPAAVEAIVKKAMAKDLAKRYPSAMALLKEIYALQQKTGWE
                     KKLLEETDSPTQVWNTAALKNTPEPGSGKRSWIIWLLSGIGMAVLTVVLFVLYTLNVF
                     TPPRPEVTVPNLVGRDLIQARQYLYQHKLPLRVVNRLYNSDYPADIIISQKPVAGQKK
                     RSDSEVEVVVSRGPELVRIPDLYGKTQIEAELALEEVGLKLGEILTGYNQEVPESTVI
                     KQLPEKDAQLERGASVSITLNVLSGDLIQVPNFIGQPLTEVQAELPSRGLVFNKATLT
                     PSDIYDVGIIVDQDPVPSFRVQQGTAIKFMVSSG"
BASE COUNT         2046 a         1763 c         2229 g         2011 t
ORIGIN      
        1 atcgggaaag ccgttaatca tgagattgag ggaccccatg tcacgatagg tgccgatctg
       61 gctaagaaat accgtgagag ttttcaggtt gttaatgcta tcgccgccca tcatggcgac
      121 gtggaagcga ataccgtcga agcagtattg gttcaagcgg cggatgcagt ctccgcagcc
      181 cgccccggtg ctcgtcgtga aaccttggaa acctatatta aacgtttgga aaagttggag
      241 aatattgccg attcgttcga aggcgtagag aaggcatatg cgattcaagc gggacgcgag
      301 atccggatta tggttaaacc tgataagatt gatgatttga actcggttcg gatcgcccgg
      361 gagatcgtga aaaaaatcga ggaggaattg gagtatcctg gccagattaa ggtgactgtc
      421 attcgggaaa ccagagccgt cgagtacgcg aagtaacaat tgggtatata ttaatattaa
      481 tcaattcggt ctggaagggg gaggagacat gaaaggcatc ttctggaaag aaggagagtc
      541 cttctccaat tccatgagtt taattgcgtt tcttctggtt aggtacccgg agattggttc
      601 agtccgtttc gacccggttc aaaaaacact ccagttttcg tttatcgtcg gtaaaccctt
      661 gacagaagag gaatttcaat cgtttaagga tcaattgtta ctcagcctga acaatatcgc
      721 tcaactacag gggcgtgatt gcggcgagat ccggatcggt tttaatgttt gcgatgattt
      781 gaccttcttg gaggttatcc gtgatcttga atcgttgaat caggaagaga tcgccttaat
      841 catcggcgtg gtcaaacagg gatttgaagg ctcgctctta cttgatcaag aggaaggtgt
      901 ccaggaagag gatgaagtga ttcaagagga aatgatcgaa cacatgctgg aggatctaaa
      961 ggattcccat ccggaaaaac gtttgattgg atttcgcgag gaaggccggg tattggtatt
     1021 taaccgggcc aatctgcata agtaatccgg tttttacaaa aaccattgaa gcacctcttt
     1081 gttgcggcaa tggttttttt ataggcatct ttcatcgtta atgggggttc tccatcaatg
     1141 gcttcaataa taaacgtttc acccatgaaa gtcttattta tcggggatgt gattggaagg
     1201 ccgggacgac gtattttgcg gactgtcctg aacgaaatca tccgggaaga ggcggtggat
     1261 ctcgtcattg ccaatggtga aaattccgcc ggtggtttcg gcttgaccgt cgaggtctgc
     1321 caggagttgc tcggttccgg ggtcgacgtg atcaccatgg gcaaccacac ctgggacaac
     1381 cgggagatat ttaaagtcct ggagacagaa ccgcgtgtgg tccgtccggc caattacccc
     1441 ggagggactc cgggcgaggg gtattatttg gcccggaccg gcaagggcga tcttgtggga
     1501 gtggtcaacc tgctgggtca ggtctttctt gaaccgctcg cttgtccgtt tcagaccgcc
     1561 gatgaatgtg tcgcagctct taaagaaaaa accccggtaa ttatcgttga catccacgcc
     1621 gaggccactt cggagaagat ggccttgggt tggtatcttg acgggaaagt aaccgcagta
     1681 atcgggacgc atacccatat ccccacggcg gatggccgga tcctgcccca gggtaccggg
     1741 tatattaccg atgccggaat gacaggaccg gtcaattcgg tattgggaat tgatccggaa
     1801 ctggtgatca agaaatttct gaccaaacgg ccggtccggt tcgagatcgc ctccgggccg
     1861 ctgcaactcg atggagtgct gcttgatatc gataccgccg gtaaaaccac ccggatccaa
     1921 tctatccacc ggaagttgga ggtttcttag agagacaact agtcgtttta ccagtatcat
     1981 caagtgtatg gctataattg cttaaaggga aagcaggccc ttcctagaat tttacccatg
     2041 aaacgccctc aggacgcgct aatacttcta accaggaggg aatcatggaa cttgtgggaa
     2101 ccaaaattcg tttgcgaccg tttgctgttg atgatttatc caaaatggcg ttttggaaca
     2161 aggatgaaga actccaggtg tatgtggact gtgatctgcc gggaaatcgg gacgaacttg
     2221 agatctggta ccatgacaat attcctgacc gttattaccg gattttcgcc atcgagacct
     2281 taaaaggcca gttgattggg gacttggaac tggatcacat ctgttggatt aaacgtgagg
     2341 ccgaactgcg aatccgtatc ggggaaaagg gctattgggg tcagggcatc ggcgccgagg
     2401 cattacggct cattttggag catgtattta cagttcgcca atttcgccgg gtttatttac
     2461 gggtctatca ttttaacgaa cgggcaatcc gctgttattc gaagcttggt ttccggccta
     2521 tcggccggct ccatcggaac acggggaact ggaaggatat tatcctgatg gagctctcca
     2581 accgtgaata tcagcgtggg ttgcgttccc tggccggatg aaaaatttat ccttggattg
     2641 acagctttta acagcatttt cccacttcta aatcggattt aacataaaag ggatttgggc
     2701 cggatttgtc gaaattatgt taaactcaaa taaggttctt ttcggaaccc tgcgggctgt
     2761 ggatggcctg ctttttccag attaaggagg gggctaaatg ccaaagatta agattatgat
     2821 tgccgataat aatcgggaat tatgcgacac ccttaccgat ctcatcactt tacaggatga
     2881 catggaattg gtcggggtag cttacgatgg gattgaagcc ctccaagtaa ttgggcaggt
     2941 gaaaccggat gtattgatcc tggacattac gatgccatat ttggatggga tcggagtcct
     3001 ggaacgatta tccgggtttc ccgagcaacc catggtaatt gtactgactg catttgagca
     3061 ggaagcaatg attcaacggt tgatcgcttt aggggcgacc tattatatgg ttaagccgtt
     3121 tgatgccccg accctttttg aacggatccg tcaattcgcc gcgggtaagg gttcagtacc
     3181 gcgccggtca aataaagccc aggaagagca atatatttac gcaggtacca accggcatga
     3241 gaaatccgtt aatcagttgg aactggaagt aacccggtta tttcatgaaa tgggaatacc
     3301 ggcgcatttc cggggttatg cttatttgcg ggaggcgatc attattgccg cccaagaggt
     3361 tgaggtttta ggaaatatta ccaaaaatct ttacccccgg atcgccgaaa agtttcgttc
     3421 tacaaccagt ggtgtggagt ccgccatccg acatacgatc gaaatcggtt gggaacgggg
     3481 aaattcggag tatatggatg agttttttgg attcgaaaat caaaaaggcc gtttccctac
     3541 cacagcttca tttatcgcca aagtcgccga taaattgcgt ctcgaatcga aagttgtctg
     3601 atctcaagat aaggtgcagg agctagtcat ctcaccgtaa agacgcgggt ggactgcgcg
     3661 tgaacgcgcg ggctataaag cgcgtttttg cggtgagctt atttataaat ggcggatcgt
     3721 tccctgcggg ttcggattga agcgaccagt aaatggaata tgaatcattt cgagaaaggt
     3781 tacataggaa ttgaaaatac taaattgctt ttgtttaatc gcgcgtgcag ccgcatacaa
     3841 tgaaagcaat ttagtacttg aaatggagaa cgaggatgct gattaagggt ttgaccaggg
     3901 aagaactgga cgagtgttta cagcggttcg cgttgccccg ttaccgggtg gatcaaatat
     3961 ggagttggct ctatcaaaaa gccgcggttt cctgggatga gatgaccaat ctaccgctct
     4021 ctttacgtca ggaccttgct gctgccggtt ttaccttaag cggtcttcat gtggtagaca
     4081 aagtaacggc cagcgacggt acaatcaaat accttttccg actggaagat gacacgacca
     4141 ttgagagcgt tttcttgcct gaaaccgacc gaaacaccgt ttgtctttcg acgcaggccg
     4201 ggtgcggcat gggttgcgct ttctgcgcta caggccaaaa tggattgacc cgtaatctga
     4261 cggcaggcga gatcattgac cagcccttat atatcggtcg tttacaacaa acccatttaa
     4321 gcaatctggt gatgatgggc cagggggagc ctttctccaa ttacgatgcg gttttaaaag
     4381 cggtgcggtt gatcaatgat ccggcgggga tgggaatggg agcgcggcat atcaccattt
     4441 ctacctgcgg tgtggtaccc gggattctta agttggctga tgagccttat caagtcaatc
     4501 tggctatttc attgcacgcg gtaaatgatg aattacgcag ccgtttaatg ccaattaatc
     4561 gaagatatcc tttagcggaa ctgttggagt cgtgccggta ttacgtggca aagaccaatc
     4621 gccgtataac ctttgagtat accatgattg ccggggtgaa cgacggccaa gccgatttaa
     4681 aaggactgtt ggccgcgcta cgcggtatgc tttgtcatgt caatttgatc ccattgaatc
     4741 cagtacccgg ttcggaatgg gaacgctccg gcaaagcccg gattcgggaa tttgaagccg
     4801 ccttaaatca ggctggaatc gaaacaacgg tccgtaagga acgtggcagt tcacttgcgg
     4861 ccgcttgcgg ccagctgcag ggtaaaaggg gtcatgattc aagaaattag gcaggatcta
     4921 gacgggggac gcatatgcgg ttgcttcaat tcatcggggg ccaaattcaa aacatagctt
     4981 cactgattca taaccggtta ctccggttaa aacacccgca aaccaagggg acagctgact
     5041 ccagtcgcgt caaggaaggg ttggatcaga cccgtttacg gcaagaaacg ggttccattt
     5101 tgcagcggat gccctcaccc gtttttcgga ccattctcag attgggccgc actgaaccaa
     5161 attgtcttac attaaggcgg caacggaaac ggactctgat ccaaccggtt cagacacgat
     5221 ggctctttac cgtggtcagc ggggtggatt atggacggca gtacatgggg atcacacgag
     5281 agattcgggt tggccggcaa cccgagaatc atattcagtt gcgggatccc aaggtttcta
     5341 ggctccatgc ggtgattcac tgccgggggg cggttttaat cctggaggat catcatagta
     5401 ccaacggtac ctggatcaat ggccggcgaa tctttagaaa acgtttgtat cccggggatc
     5461 tgatccgggt aggtgagacg gagatccggg tggttcagca aaaatagtgg atatagcttt
     5521 tgggggtgag cccatgagag ttggcgccaa aaccgatatc ggtaaagtaa gagaagtgaa
     5581 tgaagacagc tatggttatc atggccgcct atttgtactg gccgatggta tgggaggcca
     5641 cgaggcggga gaaattgcca gtgcgattgc agtggaaact gttttggcag cggatctggg
     5701 agatgcacca accacgcggt tgcatcaggc gattttagat gccaatcggg ctattttggc
     5761 cgaggtggaa aaacatcccg agcatgccgg gatggggaca accatcaacg tgctgtattt
     5821 agatattaca caagcgtata ttaccaatgt cggggatagt cgcatatatc gtctctctgg
     5881 cggacgattg aagcagttga ccgctgatga ttctttagtg gcccagctgg tcaaaaatgg
     5941 gagcattacc gaggaggagg ccaaagttca cccgcggccg aatgttctca ttcaagcact
     6001 gggtatggaa ggaaatctgg agtttgaggt aaccacggtg ggaatcgctc cgggcgataa
     6061 atttctgctc tgcagtgacg gtttaaccgg gatgcttgac gaaaaaacca ttacttccta
     6121 tctggatgcg gatatggcgc cgcaaagtat cgccgaacaa ttggtcggag cggccgatga
     6181 ggcgggcggt agggataata tcactgttat cgtagttgaa gtctaggcag cttaattttc
     6241 tattcactca agtcagggat ttctcccgtt tcgaacggat tccagcagga tttcaaggat
     6301 agatggcgaa aaagcaaagg atataaagga ttattcgcat atgcatacgc atatgcggga
     6361 accaaaccgg ttgcggctta tcccgaatgg gataaaacgg aatcgggttg aggtgatatg
     6421 attaatggtc ggtaaagtcc taggaaaccg ttaccggttg ctggagttga taggtgaagg
     6481 cggaatggct caggtatata aggcagagga ttccatactc tgccgaaccg tagccatcaa
     6541 aatcttgcgc tctcaatacg ctggcgacgc tgagttcgtg gaacggtttc gtcgtgaggc
     6601 ccgggccgca gccagccttt cccatcctaa tattgtcaat atttatgatg tgggccagga
     6661 aaacggcacg gactatattg ttatggaata tctgccgggc tgcacattga agcaggttat
     6721 caaggatgaa gcaccgcttt cggtggaacg ggctctggat ataacgcggc agattgctga
     6781 gggtttaaac catgctcatc aaagaaatat cgttcatcgt gacatcaagc cgcataatat
     6841 cctgatcaca ccggacggcc agattaaagt taccgatttc ggaatcgccc gggcgataag
     6901 tgccggaaca ttgacgcaaa tcggggaagt cgtgggctcg gtacagtatt cctccccgga
     6961 acaggccaag ggggaggcgg ccggtcccca atccgatctg tattctctcg gttgcgttct
     7021 gtatgaatta ctcaccggta ccgttccctt taaaggggat acactggtct ccattgcctt
     7081 gcaacacctc caggaagagg tggttccaat ccggcaaatc cggccggatc ttcccgcggc
     7141 ggtggaggct atcgtcaaaa aggctatggc taaagatctt gcgaagcgat atccgtcggc
     7201 catggcattg ctaaaggaaa tttacgccct tcaacaaaag acaggctggg aaaagaagtt
     7261 actggaagag accgattctc caactcaggt atggaatacg gcagcgctaa agaacactcc
     7321 cgaacccggt tccgggaaac ggtcctggat catctggcta ctgtccggaa ttgggatggc
     7381 ggttcttacc gttgtgcttt ttgtcttata taccttgaat gtattcactc caccccgccc
     7441 tgaagtgaca gttcccaatc tggtggggcg ggatttgatt caagcccggc agtatctgta
     7501 ccaacataag cttcccctga gggttgtgaa ccggctttat aattccgatt atcctgccga
     7561 tattattata tcgcagaaac ctgtagccgg tcagaaaaaa cgttccgata gcgaagtgga
     7621 ggttgtggtc agccgcggac cggagttggt gcgcattccg gacctttatg gcaagactca
     7681 gatcgaagcc gagttggctt tggaggaagt cgggcttaag ctgggggaga ttttgaccgg
     7741 ttataatcag gaggttcccg agagtaccgt aattaagcaa ttgcctgaga aggatgctca
     7801 gctcgaacgg ggggccagtg tttcaataac cttaaatgtc ttatccgggg atctgattca
     7861 ggtgcctaat tttatcggcc aaccattgac ggaggttcaa gcagagttac ccagtcgcgg
     7921 cttggtcttt aataaagcca cattaacccc gagtgatatt tacgatgtgg gcatcatcgt
     7981 ggatcaggat ccggtgcctt cgttccgggt gcaacagggg accgcaatca agtttatggt
     8041 cagttccgg
//
LOCUS       DASVMB010000526         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_725579,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000526 DASVMB010000000
VERSION     DASVMB010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725579"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VF531_14815"
     CDS             complement(<1..949)
                     /locus_tag="VF531_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEX7715286.1"
                     /translation="MGKFVDRPRYTCALGGAIGTLISLPRVVPILHAAAGCGGNIANA
                     LNGAAGYLGSGYCSGQALPSSNVYENEIVFGGESRLAEEIENTLKLVEGDLYVVVTGC
                     MVEMIGDDARNVARKFAGRRAPVLAVDSGGFKGNSFKGYDLVLGMLFREFVLKSRPKN
                     PKLLNLWGIVPVQDVFWKGNLRVLKELLTKLGYQVNTFFGESETLQNLRDAGGAVLNI
                     VVSDTAGVEPARVFEEEHGTPFISVPFPIGVRGTGQFLQAVGEALGMGETQIHQLIQD
                     EQRQFYSYLERLADVYNDLDLQRYAVIVGDSNYAQALTRF"
     gene            complement(1103..1285)
                     /locus_tag="VF531_14820"
     CDS             complement(1103..1285)
                     /locus_tag="VF531_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEX7715287.1"
                     /translation="MSPVKKDLDQEEQPKRLPLSAEDFNRLLELAGIIHYGSITLQIQ
                     DGKVIQIERHEKMRLR"
     gene            complement(1546..2481)
                     /gene="cysK"
                     /locus_tag="VF531_14825"
     CDS             complement(1546..2481)
                     /gene="cysK"
                     /locus_tag="VF531_14825"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7715288.1"
                     /translation="MAKIAKSLVDLIGNTPLLELSNYNRANHLEAKVVVKLEYFNPLS
                     SVKDRIGYAMIKDAEERGILKPDSVIIEPTSGNTGIALAFVAAARGYRLIITLPETFS
                     IERRNLLKALGAELVLTPGSEGMKGAIRKAEELAAQIPNSFIPQQFKNPANPEIHRRT
                     TAEEIWRDTDGKVDIFVSGVGTGGTVTGVGEVLKQRNPGVKIVAVEPFDSPVLSGGNP
                     GPHKIQGIGPGFVAEIINRAVIDEIFKVKNEEAFETSRKLAKTEGLLVGISSGAAAFA
                     ATELAKRPENKGKTIVAILPDTGERYLSTPLFQEN"
     gene            complement(2599..>3364)
                     /locus_tag="VF531_14830"
     CDS             complement(2599..>3364)
                     /locus_tag="VF531_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX7715289.1"
                     /translation="KQGIFKDVDACLMMHPASVTTVTEETLALDPLDFEFFGKAAHAA
                     ACPEKGVNALDGVIQLFNGINALRQQLPTDVRIHGIITHGGVAPNIIPEYAKARFFIR
                     AATRERCDEVTGKVRAIAQGAALATGTKVKIDFFQNPIDNFLTNQKLDAVFKDVIAGL
                     GESIKPRLKKGLGSTDAGNVSHVVPTIQPSVRIGPDDLVAHSIEFSNAAKSKEGDRAL
                     IISAKALALTGLILLTDEERLQSVRAEFEKNSRNRK"
BASE COUNT          805 a          931 c          771 g          857 t
ORIGIN      
        1 tgaagcgggt caatgcctgg gcgtaattgg aatccccgac aatcaccgcg taacgctgca
       61 gatcaaggtc attatagaca tcggccagcc tctccagata actgtaaaac tggcgttgct
      121 catcttgaat gagctgatgg atctgagtct ctcccatccc aagggcctca ccgaccgcct
      181 gtaggaactg cccggtaccg cggaccccga taggaaacgg cacgcttata aagggggtcc
      241 cgtgttcttc ttcaaagacc ctggccggtt ccactccggc agtatcggaa accacgatat
      301 ttaaaaccgc ccctcccgca tctctgaggt tttgcagggt ttcgctctcg ccaaaaaagg
      361 tattgacctg atacccaagc ttcgtaagga gctcttttaa aacccgcagg ttccctttcc
      421 agaaaacgtc ttgaaccggg acaatacccc ataaattaag gagtttcgga ttcttgggcc
      481 ggcttttaag aacgaattcc cgaaacaaca tccctaaaac caggtcgtat cctttaaagg
      541 agttaccctt gaaaccgccg ctatccacgg ctaaaaccgg cgcccgacgt cccgcaaatt
      601 tcctggccac attgcgggcg tcatcgccga tcatctctac catgcatccg gtaacgacca
      661 catatagatc gccctcgacc aacttcagcg tattttcgat ctcctccgcc agacggcttt
      721 ctccgccaaa gacaatctcg ttttcataaa cattggaact gggaagtgcc tgcccggagc
      781 agtagccgct ccccaaataa ccggcagccc cgttcagcgc gttggcgata tttccgccgc
      841 aacctgcggc ggcatgtaag atcggaacca cccggggcag gctgatcaag gtaccgatcg
      901 cccctcccaa agcgcaggta taacggggcc gatccacaaa ttttcccata accgaacctc
      961 ctgaccgtat ccaactttta ggtgtggagt ttaaatggaa aataaaaaag ccatgaatcc
     1021 atcgcgtgat ggattcatgg cctccagtat ctctggtcaa ccataaaatt ttttgtctgt
     1081 cttcgttact ggatggcagt atttatcgca aacgcatctt ttcatgcctt tcgatctgaa
     1141 tgaccttgcc atcctgaatc tgaagcgtta ttgaaccgta atgaataatc cccgccaatt
     1201 ctagaagccg attgaaatct tcagccgaaa gggggagccg ctttggctgt tcctcttgat
     1261 ccaggtcttt tttaaccggg ctcatctcta tacccccatg agctcaaatt aaaggcctta
     1321 ttattttggt gtcgaaggcg tttcctttga gagattgaag aaacaattat catgtcggcg
     1381 aataatgtct actatgatta tatgtttact catatattaa gttgcgtctt ttgacataaa
     1441 aattgtatct aaaaacgatg aatcggtaac cataaaagag aaaccaggca acaccgggaa
     1501 ctgaaatgat tcgtcaacca aaccggataa cgaatctccc gttttttaat tctcctggaa
     1561 aagcggagtg gagagatatc gttccccggt atccggcaat atggccacga tggtcttacc
     1621 cttattttcg ggccgcttgg ctaattcggt tgccgcgaaa gccgctgcgc cggaggagat
     1681 gcccaccagc aagccttcgg ttttcgccag tttacgtgaa gtctcaaagg cttcctcgtt
     1741 tttcacctta aagatctcgt caatcacggc ccggttgatg atctccgcca caaaaccggg
     1801 gccaattcct tgaatcttat ggggaccggg attgccgccc gacaaaaccg gcgaatcaaa
     1861 cggttccacc gccacgatct taacccccgg atttctctgc ttcagtactt ctccgactcc
     1921 ggtaaccgtc ccgccggttc cgactccaga cacgaagatg tccaccttgc cgtcggtatc
     1981 gcgccagatc tcttccgcgg tagtgcggcg gtgaatctcc gggttggcgg ggtttttaaa
     2041 ttgctgcgga atgaaggaat tcggaatctg ggccgccaac tcttcggcct tgcgaattgc
     2101 acctttcata ccctccgaac cgggcgtcaa aaccaattcg gcgccgaggg cttttaagag
     2161 attgcgccgc tcgatactga aggtctccgg caaggtaatg atcaaccgat agccccgggc
     2221 cgctgcaacg aaagccaaag cgattccggt attgccgctg gtcggttcga tgattaccga
     2281 atccggcttg aggatccctc tctcttcggc gtctttaatc atggcgtagc caatccgatc
     2341 cttgacactg ctcaaggggt tgaagtactc aagcttgacg acgactttgg cctcaaggtg
     2401 attggcccgg ttgtaattgg acaactcaag caagggagtg ttgccgatca gatccactaa
     2461 acttttcgca attttcgcca ttgaaatccc ttcttttcaa tttatagtat tcctatagaa
     2521 ttactcggtt attgatatgt taccatggtt tcaaaaaaat gtcaaccgtt tctctgctga
     2581 tctaagccga cttgaggctt atttccggtt cctgctgttc ttttcaaatt ccgcgcggac
     2641 ggattggagt ctttcctcat cggtgagaag gattaaaccg gttaacgcca gcgctttcgc
     2701 gctgataatc aatgcccggt ctccctcctt cgacttggcg gcgttactaa attcgatact
     2761 gtgcgccacc aggtcgtccg ggccaatcct gaccgacggt tgaattgtcg gcaccacatg
     2821 actgacattt ccggcgtcgg tcgatcccaa cccttttttc agccgcggct tgatgctttc
     2881 tcccaatccg gcgatgacat ctttaaatac ggcatccagt ttctgattgg tcaaaaaatt
     2941 atcgatcgga ttctggaaaa aatcgatctt gactttcgta ccagtggcaa gagccgctcc
     3001 ctgggcaatt gccctgactt taccggtcac ctcgtcgcag cgctcccggg tggcggcccg
     3061 gataaagaac ctggctttgg cgtattcggg aatgatattg ggcgcaactc cgccatgggt
     3121 aatgatcccg tgaatccgta catccgtagg caattgttgc ctgagcgcgt taatgccgtt
     3181 aaaaagttga atcaccccgt ccaatgcatt aacgcctttt tcaggacaag ccgccgcatg
     3241 ggccgcttta ccgaaaaact cgaaatccag cggatcgagg gccaatgtct cttcagtaac
     3301 cgtagtgaca ctcgcgggat gcatcatcag acaggcatca acatctttga atatcccctg
     3361 ttta
//
LOCUS       DASVMB010000527         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_653665,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000527 DASVMB010000000
VERSION     DASVMB010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653665"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1314)
                     /locus_tag="VF531_14835"
     CDS             complement(142..1314)
                     /locus_tag="VF531_14835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7715290.1"
                     /translation="MENFDFLSSTKIIFGRDTENRAGAEAKRYSSKILLHYGGGSIKR
                     IGLYDRIVQSLHEAGVEYTELSGVMPNPRLGLVHEGIKLCREEEIGFILAVGGGSVID
                     SAKAIALGVPYQGDIWDFFARKAVPERNLPVGVVLTIPAAGSEASTSAVITNEDGWLK
                     WGLNTELNRPCFAILNPELTYTLPSYQTACGAVDIMAHIMERYFTNVDHVDLTDRLCE
                     GALKTMIKNAPIALAEPDNYDARAEMMWTATLAHNDLLSTGRIGDWATHQIEQEISAI
                     YDVAHGAGLAVLFPAWMRYTFKRNLGRFIQFAVRVWDVEYDFANPETTAWEGIRRLED
                     FYRRLGMPLTLRELSVPDDRLEEMAVKAMKPWLKSVGGVSQFNRDDILNVLKLARG"
     gene            complement(1602..2201)
                     /locus_tag="VF531_14840"
     CDS             complement(1602..2201)
                     /locus_tag="VF531_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX7715291.1"
                     /translation="MLKKALICGAAFLLLSMGAKPARADEMRYIQPGDSLYKIAINSG
                     VTVYDLQQANGLSGDLIYAGDKLDIPTDSRGTRQSVDGRDVDMLARLITAEAGAEPFQ
                     GKVAVASVILNRMNDPRFPATLAKNIFKPNEFESVSNGLIWGQPPSEDSYKAAEDALK
                     GWDPTGGAKFFFNPAKVHGLSWIWTRTITGRIGNHVFGI"
     gene            2791..>3072
                     /locus_tag="VF531_14845"
     CDS             2791..>3072
                     /locus_tag="VF531_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715292.1"
                     /translation="MYRVLIVDDEKLTRFQLRRYPHWEEYGFTIAGEATDGKEALDLI
                     GKEQFDLILTDIMMPKVDGIELLKAIKKMDRHVCVIFISGYSDFSYAKEG"
BASE COUNT          772 a          760 c          742 g          798 t
ORIGIN      
        1 ttatcagtaa tctcatttcc taattgcagt agacgcggac ggggatgaat atctttttca
       61 attcgcggta gtgatagttt ggtagtgaca agttccattg aaaacacctt cttgttgccg
      121 cttgtcaaat actgtataga gtcatcccct ggccagtttc aagacgttta aaatgtcgtc
      181 ccggttaaac tggctgacgc ctcctacgct tttaagccag ggtttcatgg ccttgaccgc
      241 catctcctca agacggtcat cgggaacgct taattcccgc agggtaagcg gcattcccag
      301 gcggcggtag aaatcctcaa gccgccggat accctcccag gcagtggtct caggattcgc
      361 aaaatcgtac tccacatccc agactcttac ggcgaactga atgaaccggc cgagattccg
      421 cttaaaggtg tacctcatcc aggccgggaa gaggacagcc agtccggcgc catgggccac
      481 atcatagatg gcgctgatct cctgctcgat ttgatgggta gcccaatctc cgatccgccc
      541 ggtgctaagc aggtcattat gggccaaggt ggcggtccac atcatttccg cccgggcgtc
      601 gtaattatcc ggttcggcca gggcgatggg ggcgttttta atcatcgttt ttaaggcccc
      661 ttcgcagagg cgatcggtaa gatcaacatg atcgacgttg gtgaaataac gttccatgat
      721 atgtgccata atatcaactg ccccgcaggc ggtctgatag gatggtaagg tgtaggtgag
      781 ttcggggttt aggatcgcaa aacacggccg gttaagctcg gtatttaagc cccatttcaa
      841 ccagccgtcc tcattggtga tcaccgcact ggtgctggcc tcactcccgg ctgccggaat
      901 ggtgagtact actccaaccg gaagattccg ttccggtacc gcttttctgg caaaaaaatc
      961 ccagatatca ccctgatatg gtacccctag cgcaatggcc ttggcggagt cgatcacgct
     1021 acccccgcct acagccagaa taaagccaat ttcctcttca cggcatagtt taatcccctc
     1081 gtggaccaga cccaagcgcg gattgggcat gaccccggaa agttcggtgt attcaactcc
     1141 cgcctcatgc agggactgaa cgatccgatc ataaagaccg atccttttaa tactaccgcc
     1201 gccatagtgg agtaatattt tactggaata tcgtttcgct tcggctccgg cccggttctc
     1261 cgtgtcgcga ccgaaaatga tcttggtaga gcttaaaaaa tcgaagtttt ccattcttta
     1321 atcaccttta cctgtttggc atattattcc tcttgatgga gtccggaatg tattcatttc
     1381 tattttatca tgctttgaac tgcgattcgg taattccaca aaatttttct ccacaaacga
     1441 taaaaaaata ggaagataat ctaccgctcc atttcgcgtt tgaaatggtc cggagtggat
     1501 aagagccttg ccccataatt aagcggccca tacggaaaaa taccgtatgg gccgttgttg
     1561 aacgcccggt acgggcgtta catcttcgga aactttttaa actagatccc gaagacatgg
     1621 ttcccgatgc gcccggtgat cgtccgggtc cagatccagg agaggccatg cactttggca
     1681 gggttgaaga agaatttcgc cccgccggtt ggatcccaac ccttcaacgc gtcctctgcc
     1741 gctttgtagg aatcctcaga cggcggttga ccccagatca ggccgttgga tacggattca
     1801 aactcattgg gtttgaagat gttttttgca agtgtcgccg gaaatctcgg atcattcatt
     1861 cggtttaaga tcaccgaagc tacagcgact ttaccctgaa agggttcggc ccctgcctcc
     1921 gcggtaatga gcctggccag catgtccaca tcacggccat ccacagattg ccgggttcca
     1981 cggctatctg tcggtatatc cagtttgtct ccggcgtaaa tcaagtcgcc gctcaggccg
     2041 ttggcctgtt gcaaatcgta tacggttacg ccgctattga tcgcaatctt atacaatgaa
     2101 tcacccggct ggatatagcg catctcatcg gcgcgggcag gtttagcccc catagacagc
     2161 agcaggaagg ctgctccaca aataagcgct ttctttaaca tgttttgttt cctcctttca
     2221 aaaccgatag ccgttcctcg tcggcggcaa tccgaatttc gggttacgta tgccggctcc
     2281 ggtgcggctt ttttttcatc cattctgatt ttcacgtcac tgattcctta tcaacccgtc
     2341 attttgcatg cttggcgtat aataaacggg caaaaataca tgtctctccg gcagcttcca
     2401 atttaccggc aaaaaacatc gtatccaacg atgtaagcca ttataacaac ggcctccggg
     2461 aagatcaaaa cataaattat ggtaaactgc tgggaactca atgttgatag gaggtgaaca
     2521 cgggtgtttt tttaaagaat tcgattcaaa ttttgatcga ataaaaaaga ggaggtagaa
     2581 gcgttttttt agaataaatg atataattga taaaggataa ttttaaaagc ataattaagt
     2641 ttagagttgt gacagctgaa tgactgaagc cgtttgacgg cgggctaaaa ccggggctaa
     2701 ttaaacagga tcgggacgca ataaagacag ccatgatcaa ggatccctgt taatgaggtt
     2761 gtcaccggtc gggcctgaaa ggagagcaag gtgtaccgcg tcctgattgt tgatgatgag
     2821 aagctaaccc gttttcaact gagaagatat cctcattggg aggaatacgg atttactatt
     2881 gccggtgagg ccacggatgg caaggaagcc ctggatctta tcggcaagga acagttcgat
     2941 ctaattctca ccgatattat gatgccgaaa gtcgatggga ttgaactgct aaaagccatc
     3001 aaaaaaatgg atcgccatgt ttgcgtcatc tttatcagcg gttatagtga ctttagctat
     3061 gccaaagaag gg
//
LOCUS       DASVMB010000528         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_578707,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000528 DASVMB010000000
VERSION     DASVMB010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578707"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1047
                     /locus_tag="VF531_14850"
     CDS             178..1047
                     /locus_tag="VF531_14850"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7715293.1"
                     /translation="MEKVRIEDLKPGMVLGRTIYGTDGRILVKENAEISASIIEKLRK
                     LGLPAAYIRQTDDSDRPDLVSDATRMDLIRSLSKIDTEVRAGGDPNLGSSKIPLYDLV
                     DEIVRNQRILLGMTDIRLHNDYTYGHSVHVAIIAVKIGLQMGYNQLKLADLAVGALFH
                     DIGMTKIPLAILEKTDNLTEEEMKLIQTHAEVGYNMLRQSQDISNVAAHVAYQHHERY
                     NGSGYPRGMAGDAIHEFARIVAIADVYDSMTTEKIYRPAKPMMEAMHYVDTRKGIDFD
                     PNIVDIFEQVVAV"
     gene            1204..1575
                     /locus_tag="VF531_14855"
     CDS             1204..1575
                     /locus_tag="VF531_14855"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7715294.1"
                     /translation="MNTFSGKKELGDWGERIAAAYLKEQGYILITHNYRCPYGEIDLI
                     AKEGSVWCFIEVKTRTGQSFGRGYDSVTRMKQRHIIKVAQYYLNRAGLDEAPARFDIV
                     SIDFVAEKQYQIELIRNAFYT"
     gene            1600..>2483
                     /locus_tag="VF531_14860"
     CDS             1600..>2483
                     /locus_tag="VF531_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7715295.1"
                     /translation="MLAKIESMAVNGIDGSLVQIEVDLGGGMPSFDIVGLPDLAVKEA
                     RDRVRAAITNSGLEFHYHRIIVNLAPANIKKEGSGFDLPIAVGILAAKGDINPDKIGK
                     SVFIGELALDGSVRPVYGALPMAIAARDAQKKYLIHPAENLTEVAVIYGITPIPVTSL
                     NQVVAFLNGDLQIETIKPSPTDETVVAIEEDDLADVKGQEQAKRALEVAAAGGHNLLM
                     VGPPGSGKTMLARRLPGILPSLGLAESIELTKIYSVSGQLPPGIPLIKRRPFRSPHHT
                     TSPAGLIGGGRIPRPGEV"
BASE COUNT          658 a          542 c          657 g          626 t
ORIGIN      
        1 caccgatacc cgaggagaca tatcaattag ttgcttctat ccttgccttc atctggagga
       61 tggatcaaaa gtaccaaaac cacgggagtt aaggaatatt cgcagtatac tgtattcgga
      121 ttcatcgtgc cgcgatatgg ataacgtaat aatacaacac aagaggggtc accgtttatg
      181 gaaaaagtgc gcatcgaaga tttaaaaccg ggaatggtcc ttggcaggac aatttatggg
      241 acggacggga ggatcttggt caaggaaaat gccgagatat ctgcatcaat cattgagaag
      301 ctgcgaaaat tgggtttacc tgcggcttat atccgtcaaa cggatgattc ggatcgtccc
      361 gatctggttt ccgacgcaac ccggatggac ttgatccgta gtctctccaa gattgacact
      421 gaggtacgcg ccggaggcga tcccaatctg ggatccagta aaattccttt atacgatttg
      481 gttgacgaaa tcgtccgcaa ccaaaggatc ttattaggaa tgaccgatat ccggttgcat
      541 aatgactata cttacggtca ttcggttcat gtagcgatca ttgcagtcaa aatcggatta
      601 caaatgggat ataatcagct aaaactggct gatcttgcgg taggggccct gtttcacgat
      661 atcgggatga ccaaaattcc tctggcgatt ctggaaaaaa ccgataacct caccgaggag
      721 gagatgaagc tcatccagac ccacgcggag gtggggtaca atatgctccg ccagagtcag
      781 gatatttcca atgtagcggc gcatgtcgcc tatcaacacc atgaacggta taacggcagc
      841 ggttatcccc gcggaatggc cggagatgcc attcacgagt ttgcccgaat tgtggcgatc
      901 gccgatgtat atgattcgat gactactgaa aagatttacc gtcccgccaa accgatgatg
      961 gaggcgatgc attatgtcga tacccggaaa gggatcgatt ttgatccgaa tatcgttgat
     1021 atttttgaac aagttgttgc agtatgaatt gtgaagagtg aagtttgaag cttggtgcaa
     1081 acaaaaccca ttgagttttc acagtttcca aacccccggc gaacttgccg ggggtattta
     1141 ttttcatcgt ttcccgaggc caatttttgc aggaaattat cagaatatat agaaaatatg
     1201 ggtatgaaca cattttctgg taaaaaggaa ttgggcgatt ggggtgagag aatcgcggcc
     1261 gcttatttga aagagcaagg ctatattctg attacccata attatcgttg cccttatggc
     1321 gagatcgatc tgattgccaa agaaggctct gtctggtgtt ttattgaggt aaagaccaga
     1381 accggtcaat ccttcggccg gggttatgac tcagtgaccc ggatgaagca aaggcacatc
     1441 atcaaggtag cccaatacta tctcaaccgg gcaggattag atgaggctcc ggccagattt
     1501 gatattgttt ccattgattt tgtggctgaa aagcaatatc agatcgaact cattcgcaat
     1561 gccttttata cctgaattta tgtaacagga ggaatccttt tgcttgctaa aattgaaagc
     1621 atggcagtga acgggattga tggctccctg gtacaaatcg aggtcgattt aggcggaggg
     1681 atgccgagtt ttgatatcgt aggcttaccg gatttggcag ttaaagaagc tagggatcgg
     1741 gtgcgtgccg ccatcaccaa ttcgggtcta gagtttcact atcatcggat tattgtcaat
     1801 ctggcgccgg ccaatatcaa aaaagaaggg tccggtttcg atctgcccat cgccgtcggt
     1861 attttggcgg caaagggcga tataaatccg gataagattg ggaagtcggt atttatcgga
     1921 gaattggctt tggacgggag tgttcgcccg gtatatgggg ctctacccat ggctattgcg
     1981 gccagggatg ctcaaaaaaa atatctgatt catcctgctg aaaatctgac cgaagttgcg
     2041 gtcatctacg gaattacccc gatcccggta accagtttaa atcaagtcgt cgctttttta
     2101 aatggggatc tacagattga aacgatcaag ccctccccaa ccgatgagac cgttgtcgcc
     2161 atcgaggaag atgatctggc cgatgtgaaa gggcaggagc aggcgaaacg ggctttagag
     2221 gtggcggctg cagggggaca taatttgctg atggttggtc ctcctggatc cggcaagacc
     2281 atgctggcgc ggagattacc gggaattttg ccctccttgg gcctcgcgga atcgatcgaa
     2341 ctcactaaga tctatagcgt cagcggccaa cttccgccgg gaattccctt gataaagcgc
     2401 cggccttttc gttccccgca tcataccacc agtcctgcgg gtctcatcgg gggaggacgg
     2461 atcccccggc cgggggaggt gag
//
LOCUS       DASVMB010000529         7677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_140982,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000529 DASVMB010000000
VERSION     DASVMB010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140982"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VF531_14865"
     CDS             complement(<1..804)
                     /locus_tag="VF531_14865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7715296.1"
                     /translation="MASNRFTRWLVKRFRSGLTIMLVPNSSHGRVKSWPIPFAIILVI
                     VAMIGYNVYVYASFTAQIWQIYDYKQEISTKNHLISKLSRESQQVKPSLQKSDEINKF
                     LLQLEKEENRLTSAWERVQQKSGRKALVASRGGGYLRVEPYKMNELPAKANTTNTSLE
                     QLNHNLKQLDRFVSEESKAQKELLQELLAYESKLDHTPSIWPVTSFILSSFGMRFHPV
                     YKTYIGHQGVDLKAASGTPVRAAADGVVKFAGWESGYGNLVRINHGNGLE"
     gene            1192..1857
                     /locus_tag="VF531_14870"
     CDS             1192..1857
                     /locus_tag="VF531_14870"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX7715297.1"
                     /translation="MAEQIAAPWNLTGDGYIMSFKFPRNFIDGNCFLSDRQKELFAGG
                     TGCIMLVNYKSSNVGPYSELLFIPGKFTYQRSKLYTVSKIYVSTEASLKNGRNNWGLP
                     KELASFSFIKDGNSIEKIKITRDGATIMDIGIQASGLPLPLNTCLMPYPLIQQLDGKT
                     FFVNFKGRGTGRFGQILKVDVNQEFFPNFAFFKHVAIIKVSNFKLTFPRARIMEKPAW
                     DNE"
     gene            complement(1864..2070)
                     /locus_tag="VF531_14875"
     CDS             complement(1864..2070)
                     /locus_tag="VF531_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715298.1"
                     /translation="MIVMEYILELDSENRLPLPLDLIEHAELKSGAHFQAHLEGRKVI
                     IEYLPFSSQEQAKILDQTIDGLTD"
     gene            complement(2289..3296)
                     /locus_tag="VF531_14880"
     CDS             complement(2289..3296)
                     /locus_tag="VF531_14880"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7715299.1"
                     /translation="MANVLIHYFSGTGNSARVAAVAGDTFQELGFNPELRSVLKGSLL
                     YSSNYDSHVFTFPTYSFILPALMHRYLKHLPPGRGAKTMVLAIYGVPGYEGRTLSDAA
                     RILRRRGYDVILAESIGCPESFTQVSNPPQIDEQKQLSDYAALKVAAFVNQFVSGEPN
                     LKLCNLMNRIWTGLIGFCFTTIGRRCFGKLYVADETCNHCGECARTCPTGAIRIMGRP
                     RWNYRCQACQRCINLCPRAAIQTSCVRIGVVLAAMALSFIWVHTWVYGPAGPVQNPIL
                     QLLILLIGWLAGQIALFYIADKFLFLLELIPGVRKGFNWSYTKRFRRYLEPHFHPERR
                     A"
     gene            complement(3524..3709)
                     /locus_tag="VF531_14885"
     CDS             complement(3524..3709)
                     /locus_tag="VF531_14885"
                     /inference="COORDINATES: protein motif:HMM:NF019751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042601 - endospore-forming forespore
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, H family"
                     /protein_id="HEX7715300.1"
                     /translation="MKSEKAKAVLESHGVISVLYRGNPVWIEILQGDQARITMIDTDQ
                     RLEVPVAELELENPEEI"
     gene            3921..4181
                     /gene="mtrB"
                     /locus_tag="VF531_14890"
     CDS             3921..4181
                     /gene="mtrB"
                     /locus_tag="VF531_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HEX7715301.1"
                     /translation="MEPMDRSDRIEHYEAINGDYVVVRALEDGVTIIGLTRGRETKFN
                     HTEKLDRGEVMILQFTEHTSAMKIRGKAEIFTKFGAINSGRE"
     gene            4575..6929
                     /gene="trpE"
                     /locus_tag="VF531_14895"
     CDS             4575..6929
                     /gene="trpE"
                     /locus_tag="VF531_14895"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7715302.1"
                     /translation="MSGTYTHEEAFLQSLPLVRTTGTSSRFTVRGVEIGGPAPVVIAG
                     PCAVESQEQILSVAERVKAEGCQGLRGGVFKPRSSPYSFQGLGETGLKYLARARERTG
                     LFTVVEVTSPDQLEMATDYADVLQIGARNMQNFELLKAVGRGSKPVLLKRGLSATIQE
                     FLQAAEYILFEGNSRVILCERGIRTFETMTRNTLDINAIPLLRQLTHLPVFADPSHGT
                     GRRDLVAPVAKAALSAGAVGLIIEVHPHPENALSDGNQSLDFEEFSGLLHQISIPKPS
                     PIHFSPGEVWPTLDQFQSLAESRACRYIPVQLSFITDTETPVTAFAKLTSGPNRFLLE
                     SVERGEQVGRFSFIGWEPLFVFQARGSLVTVTRSGDNESVTDSYSVPDPLAELKRRLD
                     ELQVAQNQDQINFSHFSGGAVGFMGYDYVRRIEDLPEHAKAGSDCPDLLWMVPRHLAS
                     FDHVTHRIGLTVLTPADPDHSGAAYRNACNELEAILSRLQKSSELKPLHPQEPTATAE
                     PRYSVTQSEFETQVSRIKEYIQAGDAFQVVLSQRITQPFCGDPFLFYRRLRTINPSPY
                     LFYLDFGSLKLAGSSPEVMVKLQGDQVTLKPIAGTRPRGANSEEDRKLADELLADEKE
                     RAEHIMLVDLGRNDLGRVCRFGTVSVDPDRLMSIEVYSHVMHIVSTIHGQLLPGYSGV
                     DLLRAVFPAGTLTGAPKIRAMEIIDELEPFRRGFYGGAVGYLGSNGNLDTCITIRTVL
                     FENDSAQIQAGAGIVADSIPEREYRESLNKAGALLKALALSGEG"
     gene            6972..7601
                     /locus_tag="VF531_14900"
     CDS             6972..7601
                     /locus_tag="VF531_14900"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7715303.1"
                     /translation="MLVIIDNYDSFTYNLVQYFGELQPEYPNLLREIKVFRNDRIDID
                     GLMAITPDLMVISPGPCTPEESGVSLAAIEHFSGRIPVLGICLGHQCIAAVFGGKIIR
                     APVPVHGKFSQIRHNGRGLFRGLPQPLTAVRYHSLIVAEEGFPKELAITAHTDEGLIM
                     GVAHREHLTFGLQFHPESYFTACGKDLLRNFVEQARGAPVGRAATGNRQ"
BASE COUNT         1879 a         1828 c         2016 g         1954 t
ORIGIN      
        1 ttccaaaccg ttgccgtggt tgatccggac caggttacca taaccggatt cccaaccggc
       61 gaattttact acgccatcgg ctgcggcccg gactggagtg ccgctggcag ctttaagatc
      121 cacaccttga tggccgatgt atgttttata aaccggatga aaacgcatcc cgaaactact
      181 taaaataaat gaagtaaccg gccaaatgga aggggtgtga tcgagttttg actcataggc
      241 caggagttcc tgaagcagtt ccttttgggc cttggattcc tcggagacga aacggtccag
      301 ttgcttcaga ttgtgattta actgttccaa agaggtattg gtagtattgg ccttggccgg
      361 taattcgttc atcttgtaag gttcaacacg gagatacccg ccaccacggc ttgctacaag
      421 agctttacgg ccactttttt gttgtaccct ctcccaggcg cttgtcaaac ggttttcctc
      481 tttttctagt tgaagcagga atttattgat ttcatcactc ttttgcaggc tgggtttgac
      541 ctgttgcgat tcgcggctta atttggagat caaatggttc ttggtactga tctcttgttt
      601 gtaatcataa atttgccata tctgagccgt gaaactggcg taaacataga cattgtagcc
      661 gatcatggca acaatgacga gaataatcgc aaatgggatc ggccaacttt tgactcttcc
      721 atgggaagag tttgggacca acatgatggt caaaccgcta cggaaacgtt taacgagcca
      781 acgggtaaaa cgattgcttg ccacaggcgc ccaacctcca aacatgtgtt cttatttgac
      841 aatctggagg aaaaatatac ataggatttc gcgagggatc caaattctcc tgccaaactt
      901 attaaaaaat ccatttttga ggaaattctt cgatacttta tgtaaacctg gaattgcaac
      961 gccttcaata agaaccgttg aatagtttat agacaatccg aatgaatact ttcgattttg
     1021 ataaactgta acgggatatt tattagattt tattgtataa taaaagcaaa ccatcacccg
     1081 attcagggta attcattcgc tgtaacgtgg ggtgattttt ttggtttcgt gtaaaaatat
     1141 ccgttggaag tgaggctaat ttagccatgg acggaacagg agggtcaaaa aatggcggag
     1201 cagattgcgg caccctggaa tcttaccggg gatggctaca tcatgtcatt caaatttccc
     1261 cggaacttta tcgacggaaa ctgttttttg tccgaccggc aaaaagagct ttttgccggc
     1321 ggtaccggct gtatcatgct ggttaactac aagagttcca atgtcggtcc ttatagcgaa
     1381 cttcttttca ttcccggtaa atttacttat caacgcagca agctctatac cgtctccaag
     1441 atttatgttt caaccgaggc cagtttgaaa aatggcagga ataactgggg attacccaag
     1501 gaacttgcct cattcagctt tatcaaagac ggaaatagta tcgaaaagat caagataaca
     1561 cgggacggtg caacgattat ggatattggc attcaggcct cgggtctgcc cttgccgctc
     1621 aatacctgtc tgatgcctta tcctcttatt cagcaattgg acgggaagac cttctttgtt
     1681 aactttaaag gccggggtac cggtcgattc ggccagattc tcaaggtgga tgtcaatcag
     1741 gagtttttcc ccaactttgc attcttcaaa catgtggcca ttatcaaggt gagtaatttc
     1801 aaacttacct tcccccgggc gcggatcatg gaaaaaccgg cctgggacaa tgagtagatt
     1861 cccttaatcg gtaagaccgt cgatggtttg atccaggatt ttggcctgtt cctgactgga
     1921 aaaaggaagg tattcaatga tgactttccg cccttcgaga tgggcctgga aatgagcccc
     1981 gcttttcaat tccgcatgtt cgatgagatc caggggcaaa ggaagccggt tttcggagtc
     2041 gagttccagg atatattcca tcacaatcac ctgttttttc ctcttccaca accctttgaa
     2101 cggaatgggt gaggactgtt tatttgatta gcccgattga cgtgagcttg agaataggat
     2161 gtcaaaactg cacccgaagc atacatcaaa aatggggtac tccaaaaata aagccagaaa
     2221 aaggagcacg tgaggcctag gcctcacgtg cttgttaatc aagctttgac acggactgtc
     2281 gtgtattttt atgcccgccg ttccggatgg aaatggggtt ccagataacg ccggaaccgt
     2341 ttggtataac tccagttgaa acccttccgc actccgggta tgagctctag gaggaagagg
     2401 aatttgtcgg cgatgtagaa aagcgcgatc tgtcccgcca accagccaat gagtaagatc
     2461 aataactgta aaatggggtt ttgaaccggt ccggccggac cataaaccca ggtgtggacc
     2521 cagataaaag agagggccat tgctgcgagt accaccccaa tacgaacgca ggatgtttga
     2581 atggcagccc tggggcagag gttgatacaa cgttggcagg cctggcagcg atagttccag
     2641 cgaggccggc ccatgatacg gatcgccccg gtggggcagg ttcgggcaca ctcaccgcaa
     2701 tgattgcatg tctcatcggc gacgtataac tttccaaagc aacgccggcc tatggtggta
     2761 aagcaaaatc caatcagacc ggtccagatc cggttcatga ggttgcataa cttaaggttc
     2821 ggttccccgg acacgaattg gttgacaaaa gccgccactt tgagcgcggc gtaatcgctg
     2881 agctgttttt gttcatcgat ctgcggcggg ttcgagacct gcgtgaaact ttccggacag
     2941 ccaatgcttt cggccaagat aacatcatag ccgcggcgtc tcaagatccg ggccgcgtcg
     3001 gagagggttc gtccttcata acccgggaca ccgtatatcg caaggaccat cgtcttagcc
     3061 ccccgccccg gaggcaaatg tttcaggtat cgatgcatca atgcgggcag gataaacgaa
     3121 tatgtaggga aggtaaagac atgggaatca taattgctgc tatagaggag acttcccttg
     3181 agaacggagc gaagttcagg gttgaatccc aactcctgaa aagtgtctcc ggcaacagca
     3241 gctactcgtg ccgaattgcc cgtgccggaa aaataatgaa tcaaaacgtt cgccattttg
     3301 aacctcccgg taaagaacgt ataacattac ctactattta tttctgtctt ttgcatggat
     3361 ttcctgcttg aggatgtaaa tggaaagggt aacaggcaac aggcaatagg caatagggat
     3421 gaggcacgag gcaaaaggcg aaagataaag aagggaaaag tgaaaaggga aaggtttatg
     3481 gacatgaagc tgctttgagt ccacgctttt tctccttcaa acttcaaatc tcctccgggt
     3541 tttccagctc caactccgcc accgggactt ccaaccgttg gtcggtatca atcatggtaa
     3601 ttctcgcctg atcaccctgg aggatttcaa tccagaccgg attaccccgg tatagtacag
     3661 agattacccc atgggattcc agaaccgctt tggctttttc actcttcatc aacgggtgct
     3721 cccttcatga ggttgattga atcagggtta ctttatccgc tacccgattg tgttattccg
     3781 cttatgcttt ttagagccgg cgacacggat ccggatattt tcaccggctt tccagcgatt
     3841 cagggtactt ttacccttga catccggtat cgttgcctct ataatatagg aaataaatgg
     3901 ttttgcggga aggtattgag atggaaccaa tggaccgtag tgatcggatt gagcattacg
     3961 aagcgataaa tggcgactat gtcgttgtcc gggcgctaga ggacggggtt accattatcg
     4021 gactaacccg gggcagggag actaagttta atcataccga gaagctggac cggggtgaag
     4081 tgatgattct tcaattcacc gaacacacct cggccatgaa aatccggggt aaggccgaaa
     4141 ttttcactaa gttcggcgct ataaattccg gaagagagtg aggggccggt ttgtttttcg
     4201 gaattcctga ttattctaat gggaattccg attggaataa gttgtaagag acagtcgagt
     4261 gatctagagc accactttga gcagagtcgc acgcatgcgt gcgaaacagc gggtgtattt
     4321 gccgcttgag tcgagttttg agtaaaccca atttatgaag tcggacagaa ttggatagag
     4381 cagagtctag cggagacgag ccgagggtga tgtgatgatc gttttttaag atcaggcttc
     4441 cctaaggagg tctgatcttt tttattttca tacggcggaa gtaggaagcc gtacgactgg
     4501 aacaatcgtg tatgcacaat gcgtgttgaa tgtcatgcat tatacatatg cggtcgagat
     4561 gaggaggagc agagatgagt ggcacataca cgcacgagga ggcattttta caaagcttac
     4621 ccctggtacg gactaccggg acttcaagcc gctttacggt ccggggggtg gagattggcg
     4681 ggccggctcc ggtggtgatc gccggtccat gcgccgtgga gagtcaggag cagattctat
     4741 ctgttgctga gcgggttaag gccgaaggct gccaaggtct ccggggcggg gtattcaaac
     4801 cgcgtagttc accctatagt tttcagggtt tgggtgaaac ggggctcaag tacttggccc
     4861 gagccaggga acggaccggt ctgttcaccg tagtggaggt cacctctccg gatcaactgg
     4921 aaatggcaac cgattatgct gatgttttgc agataggcgc caggaatatg caaaatttcg
     4981 agttgcttaa ggcggtagga cgcggctcca aaccggtttt gttgaaacgg gggttgtcag
     5041 ccactatcca ggagtttctt caggcggcgg agtacattct tttcgaaggc aattcccggg
     5101 tgatcctttg tgaacggggc atccggactt ttgaaaccat gacccggaat acactggaca
     5161 tcaatgccat ccccttactc cgccaactca cccacttgcc ggtttttgcc gatcccagcc
     5221 acggcacggg tcgccgcgat ttggtggcac cggtagcgaa ggcggcgctt tcggcaggtg
     5281 cggtcggctt aatcatcgag gttcatcctc atccggagaa tgcgctttcc gatgggaacc
     5341 aatccctcga ttttgaagaa ttcagcggtt tactgcatca gatcagcatc cccaaacctt
     5401 cccccatcca tttttccccg ggagaagtat ggccgacatt ggatcagttc cagtcgctgg
     5461 ctgagagtcg tgcctgccga tatatcccgg ttcaactcag ctttattacg gatacagaga
     5521 ctccggttac tgcctttgcc aagctgacct ccggcccaaa tcgttttctc ctggaaagcg
     5581 tggaacgagg cgagcaggta ggtcgtttct cctttatcgg ctgggaaccg ctcttcgttt
     5641 ttcaggccag gggttccttg gtaaccgtta cccgctcagg cgataatgaa tctgtgactg
     5701 acagttattc agtgccggat cctttggccg aattgaaacg acggctggat gagctgcagg
     5761 tagcgcagaa ccaggaccaa ataaatttct ctcatttttc cggcggtgcc gtaggcttca
     5821 tgggttatga ttacgtgagg cggatcgaag atttaccgga acatgcaaag gccggttccg
     5881 attgtcccga tctactctgg atggttccca gacatctcgc ttcttttgat catgtcaccc
     5941 accggattgg tttgaccgtt ttgaccccgg cggaccccga ccattccgga gcggcttacc
     6001 ggaatgcctg taatgagctg gaagccatac tttcccggtt acagaaatca tcagaactta
     6061 aaccccttca tccacaggaa ccaaccgcaa ctgctgaacc gcgttattcg gttacccaaa
     6121 gcgagtttga gactcaagtc agccggatta aggaatatat tcaggccggc gatgcctttc
     6181 aggtggtttt atcgcaacga atcacccagc ccttttgtgg agatcccttc ctgttttacc
     6241 ggcggctccg gacgatcaac ccttcacctt acttgtttta cttggatttt ggttctttga
     6301 agcttgccgg ttcctctccc gaagttatgg taaagcttca gggcgatcag gtcacattaa
     6361 agcccatcgc cgggacccgg ccacgtggcg ccaattccga ggaagatagg aaactagccg
     6421 atgaactcct tgcggatgag aaggagcgcg ccgaacatat catgctggtt gatctgggcc
     6481 ggaatgatct gggccgggtc tgccgcttcg gaacggtatc ggtggacccg gaccgtctca
     6541 tgagtatcga agtttattcc catgtgatgc atattgtctc taccattcac ggccaattgc
     6601 tcccggggta ttccggtgtc gatcttttgc gggcggtttt tccggccggg acgttgacag
     6661 gagcccccaa gatccgggcg atggagatca tcgatgaact ggaaccgttc cgccgggggt
     6721 tttacggcgg agccgtgggt tatcttggat ctaatggcaa tctggatact tgcatcacca
     6781 ttcggacagt tctgtttgag aatgattccg cccaaatcca ggcgggcgca gggatcgttg
     6841 ccgactctat cccggaacgg gagtaccggg agtcgttgaa taaagccggg gccttactca
     6901 aagccctggc actctccggc gagggatgac cgctgaaagt atatcaacgt aatatagaaa
     6961 ggaggggcgt catgctggtc atcatcgata attatgattc gttcacttac aatctggttc
     7021 aatattttgg agagttgcag ccggaatatc cgaatctgct tcgggagatc aaggtctttc
     7081 ggaatgatcg gattgacatc gatggactca tggctataac accggatctg atggtcatat
     7141 cccccgggcc atgcaccccc gaggagagcg gagtttccct tgcggcaatt gagcatttct
     7201 cgggacggat tccggtgctt gggatctgcc tgggacatca atgtatcgcc gcggtcttcg
     7261 gaggaaaaat catccgggca ccggtcccgg ttcacggcaa gttcagccag atccgtcata
     7321 acggacgggg cttgttccgc gggcttcccc aaccgctaac cgcggttcgc taccattctt
     7381 tgattgttgc ggaggaggga tttcccaagg aactagcgat cacagcccat actgacgaag
     7441 gtttgatcat gggggtggca caccgggagc atctcacctt tgggctgcaa tttcatccgg
     7501 aatcctattt taccgcttgc ggtaaagatt tgttgcggaa cttcgtggaa caggcgagag
     7561 gcgcgcctgt tggtcgcgcg gcaacaggca ataggcaata gggaaaagag aaaagggaaa
     7621 aggcatgacg agtttgccac cttcgcttgc tacgactcac tcttgctctc tctggcg
//
LOCUS       DASVMB010000530         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_141398,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000530 DASVMB010000000
VERSION     DASVMB010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141398"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VF531_14905"
     CDS             <1..1393
                     /locus_tag="VF531_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX7715304.1"
                     /translation="LQFSETEQRNQGARCMDCGTPFCHSGLLIAGMASGCPINNLIPE
                     WNDLIYRGLWREAVQRLLKTNNFPEFTARVCPAPCEGSCTLGHVAAPVTVRNNECGII
                     ERAFQEGWIVPEPPAERTSKKVAVIGSGPSGLACAAQLNKAGHWVTVFERADRPGGLL
                     IYGIPNMKLDKSIVQRRVNLLEQEGVRFVTRTEVGKDYAPSKLLQEFDAVVLCGGATK
                     PRDLQVPGRELAGIHFAVDFLKANTKSLLDSDLQDRNYISARDRDVIIIGGGDTGTDC
                     VATALRQGCRSLAQFEIVPRPPMERAASNPWPQWPRLYKLDYGQEEAQEVYGDDPRLY
                     SISTKQFVGDGSGHVKEVHSVQVQWEMDVNGRQVPKEIPGTEKVWPAQLVLLAMGFLG
                     PEDTLLTGLGVERDARSNAKAECGRFTTNVKGVFTAGDMRRGQSLVVWAIHEGRGAAR
                     ECDRYLMGRTSLN"
     gene            1792..2520
                     /locus_tag="VF531_14910"
     CDS             1792..2520
                     /locus_tag="VF531_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7715305.1"
                     /translation="MEQRRNRLSIGINLLLLVLLIAAIIFLTIKYAPGITNLVKNPGK
                     FRTLLLSLGLVSIPVFIGLQAVQVVITAIPGELVQLAGGYVFGTFLGTVYSTAGIILG
                     SVIAFYIARLLGFRLIKNFLSPTDLERLDMLINSPKIEITLFLLFLIPGIPKDILVYL
                     AGFTPIDPWRFFILFTIARFPGLLATSFIGSNIQERDYLPVIIVSIIGCILFVIGLFR
                     KDWIIHKIHSLLHKESPEKDGSES"
     gene            complement(2554..3453)
                     /locus_tag="VF531_14915"
     CDS             complement(2554..3453)
                     /locus_tag="VF531_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX7715306.1"
                     /translation="MNSSVYGAFTTLRTKTNRHLVVQQNKLIVIGEQAARKGVKSLLD
                     WLNRTPKAPPQALIFISNRPAVDVLGFQPSDSNMPGLDFIATKTVLKKESHTFLIPVW
                     RFREILIDGAEDLYAPMLGLDLREGQYLQNGLAIFNGDRLVGELSRAETQSFGILTNQ
                     IKGGSISYRDGSGMWKGKFSMRNVSAKTRIKVKLNHGRPYFLIETKITGFLAELLGRD
                     HELTPGSVNQLERVIETETRQNLIKLIGKMQRLQSDGIGFGEKLRAQHYGFWKGHNWK
                     QLYPTSRFQVVVKADVIRNGVTR"
BASE COUNT          838 a          936 c          900 g          907 t
ORIGIN      
        1 ccttcagttt tcggagacgg agcaaaggaa ccaaggcgcg cggtgcatgg attgcggcac
       61 tccattttgc cattcaggcc tgctcattgc gggtatggct tccggttgcc ccatcaataa
      121 tctgattccc gaatggaatg atctgatata ccgcggtctt tggcgcgagg cggtacaacg
      181 tctgctcaag acgaacaatt tcccggagtt tacggccagg gtctgtcccg ctccctgcga
      241 gggttcctgc acgctgggac atgtcgcggc gccggtaact gtccggaata acgaatgcgg
      301 cattattgaa cgtgcttttc aagaaggttg gatcgttccc gaaccacctg cagagcgcac
      361 cagcaagaag gtggccgtca tcggctcagg cccgtccggt ttggcctgtg ccgcccagtt
      421 gaacaaggcc gggcattggg ttaccgtttt tgagcgggcc gatcgccccg gcggactgct
      481 gatatacggc atccccaata tgaagctgga taaatccatc gtgcaacgcc gggtcaacct
      541 cttggaacag gaaggggtga ggttcgtcac ccggaccgag gtaggtaagg attatgcccc
      601 atccaaactg cttcaagagt ttgatgccgt tgtgctctgc ggcggggcca ccaaaccccg
      661 ggatttacag gtccccggca gggaactggc aggaatccac ttcgcagtgg attttctgaa
      721 ggccaatacc aagagccttt tggactccga tcttcaggac cggaattata tttctgcccg
      781 ggatcgggat gtgatcatca ttggaggcgg cgataccggt accgactgtg tggctacggc
      841 gctgcggcaa ggatgccgta gcctggccca gttcgagatc gtacctcggc cgcccatgga
      901 gcgagcggca agtaaccctt ggccgcaatg gccgaggctt tataaacttg attacggaca
      961 agaggaagcc caagaggtct atggcgacga ccccaggtta tactccatca gtaccaagca
     1021 atttgtgggc gatggcagtg gccatgtcaa ggaggtccac tcggttcaag ttcaatggga
     1081 aatggatgtc aacggtcgcc aggttcccaa ggagatcccc ggaaccgaga aagtctggcc
     1141 ggcccagtta gtcttgttgg ctatgggttt tctcggaccg gaagataccc tccttaccgg
     1201 attgggtgtg gaacgggatg cccgttccaa cgccaaggcg gagtgcggcc ggtttaccac
     1261 caacgtaaaa ggcgttttca ctgccggcga tatgcgccgt ggtcagagtc tggtggtctg
     1321 ggcgattcat gaagggcggg gtgcggcccg ggagtgcgat cgctacctaa tgggccgaac
     1381 atcattgaat taaaagatcg gaagaaccat ccgggcaatg gcccggattt tttttgataa
     1441 aaccaattga tggcgcaaga tcttttcacc cttgcccagc ttcctcttgc tccgcgcgct
     1501 taataaagcg cgacctgcga gggagagaag gcgagccttc tctctcagac attctcccaa
     1561 accatccgta tctataatat ggaaacgaaa ataactatcc gacacacgcg ttttccagag
     1621 gtacataagg gcgcacttgg taaagtgcgg gagaaccccc gcttgtttca gactctaaat
     1681 tactggaata atcaatcata atatgagata atggtcctgg ctttgtctaa tgtattctgc
     1741 ccctaaaaaa acttccgatc ggcgaaaaac gattagaaaa tggagcaacc tatggagcag
     1801 cggcgtaacc gtttgtcaat tggaataaac cttttgctac ttgttttact gattgcggcc
     1861 atcatttttc ttacgatcaa atacgctccg ggcatcacga atttggtgaa aaacccggga
     1921 aaattccgga cacttcttct ttccttaggc ctcgtatcca tacctgtctt cattggcctc
     1981 caagcagtcc aagtcgtgat caccgccatc cccggcgaac tggttcaatt ggccggcggg
     2041 tatgtcttcg gaaccttttt gggtacggtc tactcaactg ccggtattat tttggggtca
     2101 gtaattgcct tttatatcgc aaggctgctt ggtttcaggc tgatcaagaa tttcctctct
     2161 cccactgatc ttgagcggtt ggatatgctc atcaacagcc caaagatcga gatcacattg
     2221 tttttgctct tcctaattcc gggaattccc aaggatatcc tggtttactt ggcgggtttc
     2281 acccccatcg atccctggcg tttttttatc ctattcacta tcgcccgatt tcccgggctt
     2341 ctcgccactt cattcatagg ttccaacatt caggaacgtg attacctgcc ggtgatcatc
     2401 gtctccatta tcggttgtat cttatttgtc atcgggctgt tccgcaagga ttggatcatt
     2461 cacaagattc attctttgct acacaaggag tctccggaaa aagacggttc cgagtcgtaa
     2521 aaaaaaacgg cctaaggccg gtttttttac gcattatctg gtcactccat tccgtatcac
     2581 atcggccttg accacaacct gaaatcgact ggttgggtag agctgtttcc agttatgtcc
     2641 cttccagaag ccgtaatgtt gtgcccgcag cttttcgccg aaaccgatac catcggactg
     2701 taaccgctgc atcttaccga tcaacttgat caggttttgc cgcgtttccg tctcgatgac
     2761 tcgttccagt tgattgacag aaccgggagt gagttcgtga tccctcccta acaattcggc
     2821 caagaacccg gtgattttgg tctcaatcaa aaaatacggt cgaccgtgat tgagcttaac
     2881 cttgatacgg gttttggcgg agacattccg catcgaaaac ttccccttcc acatccctga
     2941 tccatcccgg taggagatac tgcccccctt tatctgattg gtaaggattc caaaggattg
     3001 tgtctcagcc cgggacagtt ccccaaccag ccgatcgcca ttgaagattg ccagtccatt
     3061 ctggagatat tgaccttccc gaagatccaa tcccaacatg ggggcatata agtcctcagc
     3121 cccgtcgatc aggatctccc ggaaacgcca gaccggaatc agaaaagtat gagattcctt
     3181 tttcaatacc gttttggtgg caatgaaatc caaccccggc atattggaat cactgggttg
     3241 gaaacccaaa acatcaacgg ccggacgatt ggatatgaaa atcaaggctt gaggcggagc
     3301 cttaggggta cggttcaacc agtccagaag cgactttaca ccttttcggg cggcttgttc
     3361 accgatgacg attagtttgt tttgttgaac caccaaatgc cggttcgttt tagtccgtaa
     3421 tgtagtgaac gccccatata cactgctatt cattacccac atccttagga ggagatggaa
     3481 aatttataat attcgataaa taggaggccg tcgttaagtt tttggaaccc gcgccataga
     3541 acttttaccc cgggttcgcc atcacttttt cgaccaagaa a
//
LOCUS       DASVMB010000531         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_654494,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000531 DASVMB010000000
VERSION     DASVMB010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654494"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF531_14920"
     CDS             <1..198
                     /locus_tag="VF531_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7715307.1"
                     /translation="CRDGGRIDVRSDRHGKPQFIEVNPLAGLHPIDSDLPIICKHEGF
                     TYQELIQIIVDSAKMRMGLVG"
     gene            698..1918
                     /locus_tag="VF531_14925"
     CDS             698..1918
                     /locus_tag="VF531_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715308.1"
                     /translation="MDRLFNRLFLTLTGVAHLLWSIRIITDRTASLTSKTIVLACLVI
                     SFRMIVALVLGDHKLWIRFVLWIPCLVTAVVGIAWSGNDLFYYYWWFLLPGLLALLVF
                     PYWWSWIMAAIPGAIAIITIYFRMSVSPSIAGIILQYFLVYYGICLLAAFGFTQWRKR
                     QAEVTFLERARESTAFLAQANLRLQEYAEEVRELAKVEERNRLARDIHDTLGHTLTSM
                     ILQLGGTLELLEDNPQAAREHICAVREVAREGLHEIRASMKELRTQVVNRPCGRMLWE
                     RVAKAFADSTGVEIFVKITEEFIDIDPEINELVYRAIQEGLTNAVRHGHAEQVEITVR
                     WEEGYLLVRVSDNGHGVVQMKKGFGLLGLQERLEAVHGKLYWRSAPEWGFDLGLEIPM
                     MRRCEDGCESIGGR"
     gene            1890..2621
                     /locus_tag="VF531_14930"
     CDS             1890..2621
                     /locus_tag="VF531_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715309.1"
                     /translation="MAVKVLVVDDQKLIREGLALMLHGAEPEIILAGMAANGNEAVKF
                     LAGTPVDVVLMDLRMPEMDGITATRTIIEQYPGTRVIALTTFDEDELIFGVLKAGAAG
                     YLLKDIGKEEIITAIRQAMDGSAPISPKVAAKVIQRVARENEITKPQATSKENDGIPG
                     TPKYTQPPLTDRALKNLSQQDWEILRLTAAGLSNTEIAHRLYVSRGTIKNHLSRIYER
                     LGVSGRAEAAALFARKGSSESRDDS"
BASE COUNT          751 a          572 c          737 g          767 t
ORIGIN      
        1 tgccgcgatg gcgggagaat cgatgtaagg tccgaccgcc acggaaaacc gcaattcatt
       61 gaagtaaatc ctttagccgg acttcacccc atcgattccg atcttccgat tatttgcaag
      121 catgagggct ttacttatca ggagttaatc caaattatcg tggattccgc caagatgaga
      181 atggggttgg ttgggtagac ccgcaaagtt gaagataagg attttatcac acgaaagggc
      241 acgaaacaag ttcatgaaaa tcggagcaag gagttaaatg ggatttttga ggcgctcgtt
      301 atgggcgtct ttttattttg gtatttttga ccaatcatta taattaattg ccaatttttc
      361 gataggtaaa ataatggatt tcatttattg gtccaaaggt agattgcaaa gcaaaaggta
      421 agaatcatgt tccatatgag gtgatcggga ctatcttttg ggaaaatata aattattttt
      481 taaaacttga ttgacataga ttattttttc atattaccat gttagcatac aagcgaacaa
      541 aagaagcgag gattcatgga aaatttgggg attttataaa aaataaacga aatgaaaaag
      601 gaattactct aaggaaaatg gctgaactat taggtaattc cactctgtgg cgtgaataaa
      661 agtaaacata cagtatccca tcaaaaggag gctatctatg gatcggctgt ttaacaggtt
      721 atttttaacc ttgacagggg tagcccattt gttatggagt atacggatta ttactgacag
      781 aaccgcatct ttaaccagta aaactatcgt attggcttgc ttggttatat cattccggat
      841 gattgtcgct ttggtgttgg gcgatcacaa attgtggata cgctttgttt tatggattcc
      901 ttgcctggtt acagcggttg tggggatcgc ttggagtggc aatgatttat tctattacta
      961 ctggtggttt ttgctcccgg gtttgttggc gctgctggtt tttccatatt ggtggtcctg
     1021 gatcatggca gctattccgg gggctatcgc gatcataacg atttatttta gaatgtccgt
     1081 ttctccctca attgccggga ttattcttca gtatttcctg gtatattacg gtatttgtct
     1141 gctggcagcg tttgggttta cccagtggcg caaacgccag gcggaggtca cctttttaga
     1201 aagggctcgg gaatccaccg cctttttggc ccaagcgaat ttgcgcttac aggaatacgc
     1261 ggaggaagtt cgggaattgg ctaaagtcga agagcgaaac cgtttagccc gggatattca
     1321 cgatactttg ggacatacct taacttcaat gattttacaa ctgggaggaa cgctggaact
     1381 gctggaagac aatccgcaag ccgcccggga acatatctgt gccgtgcgtg aggtggcccg
     1441 ggaaggtctg cacgaaatac gggcttccat gaaagagttg cgtacgcagg ttgttaaccg
     1501 tccctgcggc cggatgctat gggagcgggt ggccaaagct tttgctgatt ctacaggagt
     1561 agagatattt gtaaaaatta cggaagagtt tatagatatt gatcccgaga ttaatgaatt
     1621 ggtataccga gccatccagg agggattgac caatgccgtc cgccatggtc atgccgagca
     1681 agtggaaatc actgtacgct gggaggaagg gtatctattg gttcgggtca gcgataatgg
     1741 ccacggagtt gtgcaaatga aaaagggctt cggtttgctt gggttacaag aaaggttgga
     1801 agcagttcac ggtaaactct attggcgatc cgcgccggaa tggggattcg atctggggtt
     1861 ggagataccg atgatgagga gatgtgaaga tggctgtgaa agtattggtg gtagatgacc
     1921 agaagctgat ccgggaaggt ttggcgttga tgctacacgg agcggaaccg gagatcatat
     1981 tggcggggat ggcggctaac gggaatgagg ccgtcaagtt tttggcaggt acacctgtgg
     2041 atgttgtgtt aatggatttg agaatgccgg aaatggatgg tattactgcc acccgaacca
     2101 tcatcgaaca gtatcccggg acgagagtta ttgctttaac gacatttgac gaggatgaac
     2161 tgatcttcgg ggtactcaaa gccggtgccg ccggatatct gctcaaggat atcggaaaag
     2221 aggagataat taccgccatt cgccaggcca tggacggcag tgcccccatt tcgcccaagg
     2281 tggcggctaa ggttatccag cgggtcgctc gtgagaatga aatcaccaaa ccgcaggcga
     2341 cttcaaaaga aaatgacggc atccctggaa cccctaagta tacccaacct ccccttacgg
     2401 atcgagccct aaagaacctt tctcaacagg attgggagat tttacgttta acggctgccg
     2461 gcctaagtaa caccgaaata gcccaccggt tatatgtcag ccggggaacg atcaaaaacc
     2521 atctcagtcg catttacgaa cggcttgggg tctccggacg ggccgaggcg gcagctcttt
     2581 ttgcgcgtaa aggctcgtcc gaatcccggg atgatagctg atgctccttt tcaccgccac
     2641 cccgttttca gttgatttaa tgacttctgc ccttttccac cccacccacc cccatgccaa
     2701 ctggccaaaa tataccgttt taagccggat caacgtttct aaattggcct gatggcatct
     2761 ttttttaaaa aaggtccgat gttacaatca aatcagcaat attgtcatgc caaaagctta
     2821 ttgatat
//
LOCUS       DASVMB010000532         7260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_141746,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000532 DASVMB010000000
VERSION     DASVMB010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141746"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VF531_14935"
     CDS             <1..577
                     /locus_tag="VF531_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7715310.1"
                     /translation="LQVWLERYGSEVAFQIEPGRYVVAESGVLLGTVHSVKESYGTRY
                     VGTDLGFNVLARPVLYDAYHEMEAYDAAGVPKNAEYENVTVVGNICESGDILARERNL
                     PRMEPGDLIGVLDAGAYGYSMCSNYNNRLRPAEVLIRMDGTPALIRRRETLKDLSRLD
                     PDLSDTIHSSEGDDEIEVRGARRRAVGGQVF"
     gene            528..1757
                     /locus_tag="VF531_14940"
     CDS             528..1757
                     /locus_tag="VF531_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7715311.1"
                     /translation="MRCEEHVAERLGGRFFETAGASYKFARIKEWKARAIASHKDIPL
                     IDLGVGEPDQPADPRIVEVLAENSGLPGNRFYADNGIPEFQIAAADYLKRVYGVTGLD
                     PLSQILHGIGSKSILAMLPLGFINPGDVALTTVPGYPILATHTHYLGGSVYNLPLRPE
                     NGFLPDFNEIPPTVLRRAKLLYLNYPNNPTGRSATLEFYGEVIRFAERNGIVVVNDAS
                     YAALTLDGSPPLSFLSLPGASEVGVEVHSLSKAFNMTGWRLGFLAGNATVIAAYGRIK
                     DNTDSGQFRAIQLAGAYALNHPELTEVNCSRYSRRFDLLVPALNEVGFTATKPRATFY
                     CYLPSPWGNGSGVRFATAAAAAEFILREALISMVPWDDAGAYLRLGVTFAAPNIEAER
                     QVVGEIKDRLSRLHLIF"
     gene            1987..2265
                     /locus_tag="VF531_14945"
     CDS             1987..2265
                     /locus_tag="VF531_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7715312.1"
                     /translation="MKDFPVQTFYKNQVIFEEGTPANVAYLLKAGRVELSVQGKEKVV
                     LAELKPGDIFGETALLLREGRRTETAVALEEAGLIVIYRTAFQEYLEQ"
     gene            2296..2595
                     /locus_tag="VF531_14950"
     CDS             2296..2595
                     /locus_tag="VF531_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715313.1"
                     /translation="MAERQQATASRLLDVTDLIEDIVALLKLLTTQGVTTIQYDPFIQ
                     SAAEFLGADPSELAERLRRLEKLNHFDFMVDAEGHKIIKLPEPKSGEGTSSDMVG"
     gene            2812..4506
                     /locus_tag="VF531_14955"
     CDS             2812..4506
                     /locus_tag="VF531_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7715314.1"
                     /translation="MRDLIGIDVGGTNTDAVLVHEGKVVATARAATDRSRLLAGTAAV
                     LRELLRSVSKAGSGEIELHLSTTLSTNAIVERRGDPTAVLAVPGPGLNPADLGFGFPI
                     HQLSGAIDHRGREVTPLTDTEVRSAIREANLEGARALAVVGKFSTRNPCQELEIEQIA
                     RSETPGFGSVTLGHRLSGRLNFPRRITTAYLNSNVAGIQTDFARMVRELLTETAITGP
                     VFLLKADGGTLRLEDATARPVETILSGPAASIMGTLALTREPLNNAVILDIGGTTTDI
                     SVLVNGEPLNERNGAEISGYKTSVPALFSRSIGLGGDSKVVWENGKIRIGPGRDGPPA
                     IFGGPVVTPTDAVAALGLAPLGEVFRGLAALEPLAVQAGISPAELAEKVVAEFCSLAA
                     SAVRGVYRDLASRPVYTVSEVLAPCNLKPDRIIGLGAPAATYIPLIAVKLDVPYTVLP
                     YPEVANAVGAAASRPTAAITLHADTALGRVVIPELDYSDTIDRPLLYETKQARAEALA
                     HTRELAARIGVATDGAAAEIVHEEVFNMVRGFHTVGRIYTIQAQLRPGVIPIRIDR"
     gene            4520..5869
                     /locus_tag="VF531_14960"
     CDS             4520..5869
                     /locus_tag="VF531_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7715315.1"
                     /translation="MLQTKHQIGLVFFPAFDWAIEPSHPEREERLLYTRDQIVEEGLL
                     DLPGMVELRPKLATVAEVSRAHICVPDVGAQATDSHLIAAGGTIVAADAVMEHQVKRC
                     FALLRPPGHHAMRIVHGCRGFCTINHEAIMIEHIRQKYGSHLKIAVVDTDAHHADGSQ
                     DIYYNDPEVLHICLHQDGRTLYPGTGDLNELGGPGALAMNLNVPLPPGSGDNEILYVV
                     ENLALPVLEDFKPDILINAAGQDNHYTDPLTDMRVTAGGYARLTEILAPDIVVLEGGY
                     SIEGALPYVNVGIILALAGLDYSKVREPDWEPSLSDRSRPVNPAVRKGVEYLREIWRH
                     REDLDRDRIFGKGPFFKRNRKIYYDTDQIEEEQAEEIRRCTHCSGYRVIHSRALRGYG
                     PERRIAAVLIPRHACPECRGAAEAAFVKLTAGPDQQYVYLQDPDGDRFEAKGSSSER"
     gene            5996..>7260
                     /locus_tag="VF531_14965"
     CDS             5996..>7260
                     /locus_tag="VF531_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7715316.1"
                     /translation="MRKLLLSLLTGVLIIMQAGTIAAEKSSLDISGNWLGVLKVPGAE
                     LRLVLKVTKNADGSFSSKLDSIDQGANDIPVAQTILKDGKVHLDLPVIQGTFDGTLNE
                     AGTEITGEWKQGTAFPLIFKRTAEVVELRRPQEPKPPFPYNEEEVSYLNQAAGIRLAG
                     TLTTPKEKGPFPAVILITGSGAQDRNETLMNHKPFWVIADYLTRRGIAVLRVDDRGIG
                     GTSGRVDQSTSPDFAGDVLAGVEFLKSRKDINLRQIGLIGHSEGGIVAPMAAVRSKDV
                     AFIILLAGPGLSGEEIIRKQSDLIIRAGGGTDKDLAAQQGYLENIFAICRMNEPEARK
                     IKLHEYAEQLFSGMSDTEKQATGNSPAVIEAQLQSAITPWYRFFIDYDPVPTLKKVTC
                     PVLALNGSKDLQVPPKEDLAAIANALKEAG"
BASE COUNT         1621 a         1839 c         2158 g         1642 t
ORIGIN      
        1 gcttcaggtt tggctggagc gttacgggag cgaagttgcc tttcagatcg agcccggccg
       61 ctacgtagtg gcggagagcg gagtcttgtt gggaacggtc cattcggtca aggaaagtta
      121 cggcacccgc tacgtaggga ctgacctggg cttcaacgtg cttgcccggc cggtactcta
      181 cgatgcctac catgagatgg aggcctatga tgcggcgggt gttcctaaaa acgcggagta
      241 cgaaaacgtc accgtggtcg ggaacatctg cgagagcggt gatattctgg cacgggagcg
      301 aaacctgccc cggatggaac cgggggactt gatcggtgtt ttggatgccg gggcttacgg
      361 atactcgatg tgttccaatt ataacaaccg gttgcgtccg gcggaagttt taatccgaat
      421 ggacgggact ccggccctga tccgcagacg ggagacttta aaggatctat cccggttgga
      481 tccggatctt agcgatacca ttcactcaag tgaaggagat gatgagattg aggtgcgagg
      541 agcacgtcgc cgagcggttg gggggcaggt tttttgaaac agccggcgcg tcatataagt
      601 ttgcccggat caaggaatgg aaagcgcgag ccatagccag tcataaggac attcccctga
      661 tcgatctagg agtcggcgaa ccggatcaac ccgcagatcc ccgtattgta gaagtattgg
      721 ctgaaaactc gggtctaccc gggaaccgtt tttacgccga taatggcatt cctgaatttc
      781 agatcgctgc agcggactat ctaaaacggg tatacggtgt aaccggcctt gatccgttaa
      841 gccaaatctt gcacggcatc ggttcgaaat cgatcctggc catgttaccc ctgggtttta
      901 tcaacccggg cgacgtggcc ctgaccacgg ttcccggtta tccgatcctg gccacccata
      961 cccattactt gggaggcagt gtctacaacc tcccgttacg tccggaaaac ggctttctac
     1021 ccgattttaa cgagattccc cctacggtgc tgcgccgggc caaactgtta tacctgaact
     1081 atccgaataa tcccaccgga cgaagcgcga ctctggagtt ttacggcgaa gtgattcggt
     1141 ttgcggaacg gaacggaatt gtggtcgtca atgatgcttc ctacgcagcg ctgacgttgg
     1201 acggctcgcc accgctgagt ttcctgtcgt taccgggagc cagcgaggtt ggggtggagg
     1261 tccattcgct ttcaaaggct ttcaatatga caggttggag gttgggtttt ttggccggta
     1321 acgcaacggt gatcgccgcg tacggccgta taaaggataa tacggattcc ggccagtttc
     1381 gtgcgataca gttggccggg gcatacgccc tgaatcatcc ggagctaacc gaggtaaatt
     1441 gcagccgtta ttcacggcgc ttcgatctgc tggttccagc cctgaatgaa gtggggttca
     1501 cagcgacgaa acctcgagcc accttttatt gctatctccc cagtccctgg gggaacggga
     1561 gcggagtccg gttcgccacc gcggcggccg cggccgaatt tattctgcgg gaggctttga
     1621 tctctatggt tccctgggat gatgccggag cttacttacg actgggggtc acctttgctg
     1681 cgccgaatat tgaggccgaa agacaagtag tcggggagat caaagaccgc ctttccaggt
     1741 tacacttaat tttttaaaaa catcccgtgc cagaaattcg gtttttcatt ttctctttac
     1801 cctttataat ttgtctcctg cctctggtga atgatggtat aatagatctg attataccga
     1861 agttgcagcc gttaccatct tagatgcagc gtggatggat tgagaaattc cggatgatcc
     1921 gtctcccgcg taccctcatt gctgcgctgt aacacgctca tcgctcatca tacgggaggg
     1981 tcaagcatga aggattttcc ggtccagact ttctacaaga atcaagtcat ctttgaggaa
     2041 ggcaccccgg ctaacgtggc ttatctttta aaagcgggcc gggtggagct ttcggtccag
     2101 gggaaggaaa aggtcgtact cgcggagtta aaaccggggg atattttcgg agaaacggct
     2161 ctgcttctta gggagggccg acgtaccgaa accgcggtcg ctctggagga agcggggctg
     2221 atcgtgattt ataggacggc atttcaggaa tacctggagc agtaaccgcc ggtcatcagc
     2281 aagctagtca acggtatggc cgagcggcag caggcaacag cgtcccggct gttggacgtg
     2341 accgatctga ttgaggatat cgtcgcgttg cttaagctgc ttacgaccca aggggtaact
     2401 accattcaat atgatccgtt tattcaatct gcagccgagt ttttgggggc tgatccgagt
     2461 gaattggcgg aaaggttacg ccgtttagag aagttgaatc attttgattt catggtggat
     2521 gccgaaggcc ataagatcat taaactacct gagccaaagt cgggagaggg aacgtcctcc
     2581 gatatggtag ggtaggcgtg agggatggag ggcacatttg aaagcttacg actcactctc
     2641 actctcgctc tctctggcgt aaggcgccaa agagagtaac ccagatcctt cgatgttttt
     2701 ccagtcttga atctacctct cccctctttc ccgcaagcgg gagaggggag caagaagggt
     2761 gagtcgcttt ttgacttttg acctgaatca taaagaaggc agatcaatcc aatgcgcgat
     2821 ttaatcggga tcgatgtggg cggcaccaat accgatgctg tcttggtgca tgaggggaag
     2881 gtcgtggcta cggccagagc agcgacggat cgctcccggc tattggccgg aacggcggca
     2941 gtccttcgtg aattgctccg atccgtatcg aaagccggtt ctggagaaat tgaacttcat
     3001 ctctccacca cgctgtcgac gaatgccatt gtcgagcggc gcggcgatcc cacggcggtt
     3061 ttggctgtac ccggccccgg gctaaacccg gctgatctgg gatttggttt tcccattcat
     3121 caacttagcg gggctatcga tcatcgtggc cgggaggtga cccccctcac cgatacagaa
     3181 gtgcgttccg caattcggga agcgaacctg gagggagcca gggcattggc cgtggtgggt
     3241 aagttctcca cccgtaatcc ctgtcaggag ttggagattg agcagatagc caggagtgaa
     3301 actccgggat tcggctcagt aaccctcggc caccgtcttt caggacggct taactttccc
     3361 cgccggatca ccacagccta tttaaactcc aatgtcgccg gaattcagac ggatttcgcg
     3421 agaatggttc gggagctgct gacggaaacg gccattaccg ggccggtctt tcttttaaaa
     3481 gcggatggcg gtaccttaag actggaagat gcaacagccc gtccggtgga aacgatcctt
     3541 tccggaccgg ctgcaagtat catgggtacc ttagcgctta cccgagaacc cttgaataac
     3601 gcggttattt tggatatcgg cgggaccact accgatattt cggtactggt gaacggggaa
     3661 cctttgaatg aacggaacgg agcagagatt tccggataca aaacctcggt tccggcgcta
     3721 ttttcccgtt ccatcggttt gggtggagac agcaaagtgg tttgggagaa cggaaagata
     3781 aggatcggcc cgggacgtga tggaccaccc gccatcttcg gcggtccggt ggtaacgccg
     3841 accgatgcgg tggctgcctt gggtttggcg ccattaggcg aagtcttccg cgggctggcc
     3901 gctttggaac ccttggccgt tcaggcggga ataagcccgg cggagcttgc cgaaaaagta
     3961 gtggcggaat tttgcagttt ggccgcttcg gccgtgcgcg gagtttaccg ggatctggcc
     4021 tcccggccgg tttatacggt ctccgaggtg ttggcaccat gcaacctgaa accggataga
     4081 atcattgggc tgggtgctcc ggccgcaacc tatataccgc ttattgccgt caagttggac
     4141 gtgccttata cggtgctacc gtacccggaa gttgcaaacg cagtgggtgc cgcggctagc
     4201 cggcctacag ccgcgataac cctgcatgcc gatactgcgc tgggaagagt ggtcattccg
     4261 gagcttgact attccgatac gatcgaccgg ccattgcttt acgaaaccaa acaggcccgg
     4321 gcggaagcgc tggctcatac aagggagttg gcagcccgga tcggagtcgc tacggatggc
     4381 gcggcagccg agattgtgca tgaagaagta ttcaatatgg tacggggatt tcataccgtg
     4441 ggaaggatct acaccatcca ggcccaactc cggccgggtg tgatcccgat ccggatcgat
     4501 cgatgaggga gcgaagatca tgctccagac aaaacaccaa atcggcctgg ttttttttcc
     4561 cgcctttgac tgggccatcg aaccaagtca tccggagcgg gaggaacggc tcctatatac
     4621 cagggatcag atcgtagagg aaggactcct cgatctaccc ggaatggttg aactccggcc
     4681 taaactcgcg accgtggccg aagtgagccg ggctcatatc tgtgtccctg atgtcggtgc
     4741 gcaggctacg gattcacatt tgattgcagc cggtggtacc attgttgcgg cggatgcggt
     4801 catggagcat caagtgaaac ggtgcttcgc gctactccgg ccccccggcc accacgcgat
     4861 gcggatcgtc cacggttgcc gcggcttttg caccatcaat catgaagcga tcatgatcga
     4921 gcacatccgg caaaagtacg gctctcatct caagattgca gtagtcgaca ccgatgccca
     4981 ccacgccgat ggcagccagg atatttatta taacgatccg gaagtgcttc acatatgtct
     5041 tcatcaggat ggccggaccc tttaccccgg tactggggat ctcaatgaac tgggaggccc
     5101 gggggctctg gcgatgaatc tgaatgtgcc gcttcctccc ggcagcggag acaacgagat
     5161 tttgtatgtc gtcgagaatc tggctctgcc ggtactggag gatttcaagc ctgacatcct
     5221 gatcaacgcc gccgggcagg acaatcatta taccgatccg ctgaccgata tgcgggtaac
     5281 tgccggcggt tacgcgcggc tcaccgaaat tttagccccg gatatcgtcg ttctggaagg
     5341 cggttattcc atcgaagggg ctttgcccta tgtgaatgtg ggcatcatct tagccctcgc
     5401 ggggttggac tattcgaagg tccgggagcc ggactgggaa ccttcgctca gtgaccgttc
     5461 ccgcccggtg aatccggcgg ttcgcaaggg agtagagtat ctacgcgaga tctggaggca
     5521 tcgcgaggat ctcgaccgtg accggatctt cggtaaaggg ccgttcttca agcgaaatcg
     5581 taaaatctat tatgacaccg atcagataga agaggagcag gcggaggaga tccgccgctg
     5641 cacccattgc agcggttatc gggtcattca ttcccgggct ttacggggct acggcccgga
     5701 gcggagaatt gctgcggtgt taataccgcg gcatgcctgt cccgagtgtc gcggggcggc
     5761 ggaagcggca tttgtcaagc ttacagcggg acctgaccag caatatgtct atctgcagga
     5821 tccggatggg gatcgctttg aggcaaaggg ttcctcttcg gaaagataag ccgatggcca
     5881 tcgctcatcg gctggtcccg atgttatctg cgggtggagg tggattttga atatatcgag
     5941 cgggatttgg aaggctaaat gaatctaagc gtaggccgga agggagaatg agccaatgag
     6001 aaaactgtta ctctctttat tgacgggggt tttgatcatc atgcaagcgg gaaccatcgc
     6061 ggcggaaaag agttctttag atatctccgg aaactggctc ggcgttttga aggttcccgg
     6121 ggcggagttg cggctggttc ttaaagtgac gaaaaatgcg gatggtagtt tcagcagtaa
     6181 actggatagc atcgatcagg gtgcgaatga tatcccggtg gctcagacga ttctcaagga
     6241 tggcaaggtg catctggacc tgccggtgat ccaagggacg tttgatggga ccttaaatga
     6301 ggcgggaacg gaaatcacgg gtgagtggaa gcagggcacc gctttcccat tgatcttcaa
     6361 acggacggca gaagtcgtag agttaagacg tccccaggaa cccaaaccac cttttccgta
     6421 taatgaggag gaagtctcct atctcaatca ggctgccggg attcgactag cagggaccct
     6481 gactacgccc aaggaaaaag gtcctttccc cgcggtcatt ctgatcaccg gttccggggc
     6541 tcaggatcgg aatgaaaccc ttatgaatca taaaccgttt tgggtgattg ccgattacct
     6601 gacccggcgc ggtatcgcag tcttacgggt tgacgaccgg ggcatcggcg gtacatccgg
     6661 ccgtgtcgac caatccacca gtcctgattt tgccggtgat gtgctggcag gagttgaatt
     6721 cttaaaaagt cggaaggata tcaatctccg gcagatcggc ctaatcggcc atagcgaggg
     6781 cggtatcgtc gcccccatgg ccgcagtccg ttccaaggat gtggccttca tcattttgtt
     6841 ggcaggaccc gggttgagtg gcgaggagat catccggaaa caatcggatc tgattatccg
     6901 agccggcgga ggtacagaca aagatttggc cgcgcaacag ggttacttgg agaatatctt
     6961 cgcaatttgc aggatgaacg aacccgaggc aagaaaaatc aaacttcacg agtatgcgga
     7021 gcagttattc agcggaatga gcgacacgga aaagcaggcg acgggtaaca gtccggcggt
     7081 aatcgaggct caactccaga gcgcgatcac cccgtggtac cggttcttta tcgactatga
     7141 tccggttccc acgcttaaaa aagtaacctg tcccgttttg gcgctgaacg gttccaagga
     7201 tctgcaagtt ccgcccaagg aggatttggc ggcgattgcc aacgccttga aggaagcggg
//
LOCUS       DASVMB010000533         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_579488,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000533 DASVMB010000000
VERSION     DASVMB010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579488"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF531_14970"
     CDS             complement(<1..270)
                     /locus_tag="VF531_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7715317.1"
                     /translation="MKKIISLILAVMALGSAVILSGCGEKQNVLNVYNWGDYIDESVL
                     PEFEKKFNVKINYDTFTTNEDMYVKVNAGGSHYDILVPSDYMIKRM"
     gene            complement(276..1067)
                     /locus_tag="VF531_14975"
     CDS             complement(276..1067)
                     /locus_tag="VF531_14975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715318.1"
                     /translation="MVRSFLRRGYIFLIYAFLYAPIIVLALYSFNSSRSRGSWDGFTL
                     NWYAAMFRDRQILTSLYYSLIIGVIASCAATMVGTMAAFGIRNMKPLPQATVMNLTYL
                     PVLNPDIVTGISFMLLYIFFRMQLGFATLLLSHITFNIPYVILSVLPRVKQLDQSVYD
                     AALDLGATPGLAFRKVVLPEIMPGVLTGWLLSFTLSLDDFVISFFTTGSGVSSLPITV
                     YSMARRGINPKINALSTLMFVSILALLVIVNIGMARQNTAKSKED"
     gene            complement(1061..1885)
                     /locus_tag="VF531_14980"
     CDS             complement(1061..1885)
                     /locus_tag="VF531_14980"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715319.1"
                     /translation="MRKTWGAYPYVFWMAIFTVIPMILLIYFSLSVKTPAGIRFSWDN
                     FHRFLEPVYIKVLIRSLVLAGVSTLFCLLLGYPAAMILAGRRLNYQNTLVLLMVLPMW
                     MNSLLRTYAWLTILERKGLLNTLLGWIGLGPFNLLYNDFAVTLGMVYNFLPFMVLPIY
                     SVLSKIDHSLIEAAQDLGANPFLTFRKVIFPLSLPGVTSGITMVFMPAVTTFVISRLL
                     GGGQYTLIGNLIESQFLTVGDWNFGSAISMIMMFIILVGMGFTSRYETKQEGGGLW"
     gene            complement(1885..2928)
                     /gene="potA"
                     /locus_tag="VF531_14985"
     CDS             complement(1885..2928)
                     /gene="potA"
                     /locus_tag="VF531_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7715320.1"
                     /translation="MAETLISLLHVSKDYNGSEAIKDVTLHIKRNEFFTMLGPSGCGK
                     TTTLRIIGGFEIQSEGQVLFAGVDIDSLPPYKRKVNTVFQKYALFPHMNVYENIAFGL
                     RIKKMDKKIIDQKVGQMLELVSLSGYERRSIHSLSGGQQQRIAIARALINEPEVLLLD
                     EPLGALDLQLRKEMQLELKAMQQRLGITFLYITHDQEEALTMSDTIVVMDQGRIQQIG
                     TPESIYNEPVNSFVAGFIGESNIVDGTMLEDLLVQIAGRQVRCSDKGFAANEPVDVVI
                     RPEDWEIVNADEGIFSGTVRSATFKGVHYEMIVSAQGIDWRIHNTKMWPSGARIGLAI
                     DPFNIHIMKKVTA"
BASE COUNT          870 a          803 c          848 g          785 t
ORIGIN      
        1 catccgctta atcatgtagt ccgaaggcac aagaatatca taatggctcc cgccggcgtt
       61 gaccttcaca tacatatctt cgttggtggt aaaggtgtcg taattgatct tcacattgaa
      121 tttcttttcg aactccggca gtaccgattc gtcgatgtaa tcgccccagt tgtaaacgtt
      181 cagcacattc tgtttttccc cacagccgct caaaatcacg gcggatccca gcgccatcac
      241 ggccaggatc agagagatga tttttttcaa cttattcagt cctccttgga tttggctgta
      301 ttttgtcttg ccataccgat gttgacaatg acgagtagcg ccaggatact tacgaacatc
      361 agggtggata gggcgttgat cttagggttg atcccgcgcc gcgccatgga ataaacggtg
      421 atcggaagac tggaaactcc cgacccggtg gtgaagaaac tgatgacgaa gtcatccagg
      481 gacagggtaa aagagagtag ccacccggtg agtaccccgg gcataatctc cggaagcacc
      541 accttacgga aagccagtcc cggggtggcg cccagatcta gtgcggcgtc atatacactc
      601 tgatccagtt gtttcacccg gggcaacacc gacaggatca cgtaagggat gttgaaggtg
      661 atatgggaca ataacaaggt tgcaaaaccg agttgcatac ggaaaaagat atagagcagc
      721 atgaaagaga taccggtgac gatatccggg ttaagcaccg gcagataggt taggttcatt
      781 accgtcgcct ggggcagggg cttcatattc cggattccaa aggcagccat ggtgcctacc
      841 atagtggccg cgcaagaggc aataaccccg atgattaacg aataatacaa agaggtcaat
      901 atctggcgat cccggaacat agcggcgtac cagttgaggg tgaaaccatc ccaggagccg
      961 cgggagcggg aactgttgaa cgagtatagg gcaagtacga tgatgggcgc atacagaaag
     1021 gcgtaaatta aaaagatgta accgcgtctc aggaaactcc tcaccacaat cccccgcctt
     1081 cctgcttggt ttcatagcgt gatgtaaatc ccattcccac caggatgatg aacatcatga
     1141 tcatggatat ggcggaaccg aagttccagt cgcccacggt caggaattga ctctcgatca
     1201 agttgccgat gagggtatat tggcctccgc ccaaaagccg ggagattacg aaggtggtca
     1261 ctgcgggcat aaagaccatg gtaatcccgg aagtgactcc cggcaggctg agcgggaaaa
     1321 ttaccttgcg aaacgttaag aaaggattgg cgcccaaatc ctgggccgcc tcgatcaggc
     1381 tatgatcgat cttgcttaag acagagtata tcggaagcac cataaaggga aggaaattat
     1441 agaccatgcc caaagtaacc gcaaaatcgt tataaaggag gttgaagggc cccaggccga
     1501 tccaacctaa aagcgtgttc agtaatcctt ttctctccag aatggtcagc caggcgtagg
     1561 tccgcaagag tgaattcatc cacatcggga ggaccatcag caggaccagg gtgttttgat
     1621 aatttaatct ccggccagcc agaatcatcg ccgcgggata acccagaagc agacagaata
     1681 aggtactgac tccggccagg actagcgagc ggatgagaac cttgatatag accggttcca
     1741 ggaaacggtg gaagttgtcc cacgagaaac ggatcccggc gggggtcttc acggataaac
     1801 tgaaataaat caaaagaatc atgggaatga ccgtgaaaat ggccatccag aagacgtagg
     1861 ggtacgcacc ccaggttttc cgcatcatgc cgtcaccttt ttcatgatgt gaatattgaa
     1921 cggatcgatg gcaaggccga tccgggcacc gctgggccac attttcgtgt tgtggatccg
     1981 ccagtcgatt ccttgggcgc tcacgatcat ttcgtaatgt acgcctttaa aggtggcgga
     2041 acggacggtt ccgctgaaga ttccttcatc cgcgttcacg atttcccaat cctcgggacg
     2101 gatgaccacg tctaccggtt cgtttgcggc aaatccctta tcggaacaac ggacttgcct
     2161 cccggcgatc tgcaccagga gatcttcaag catggttccg tccacgatgt tgctctcacc
     2221 gatgaacccg gcaacaaagg agttaaccgg ctcgttgtat atggattcgg gggttccgat
     2281 ctgctgaatc cggccttgat ccatgaccac gatggtgtcc gacatggtca gcgcttcttc
     2341 ttgatcgtga gtgatgtaaa gaaaggtaat tcccagccgt tgctgcatcg ctttcaactc
     2401 cagctgcatc tctttgcgga gttgcaagtc cagggcgccc agaggttcat cgagcaacag
     2461 tacttcgggt tcgttgatca aagcgcgggc gatggcgatc cgctgttgct ggccgccgct
     2521 gagcgaatgg atcgagcgcc gctcgtaccc ggagagcgag accaattcca acatctggcc
     2581 gaccttttgg tcgatgattt ttttgtccat cttcttgatg cgcaatccga aggcgatgtt
     2641 ctcatagacg ttcatatgag gaaataaggc atatttctga aataccgtgt tgacctttct
     2701 tttgtacgga ggtagcgagt cgatatccac tccggcaaag aggacctggc cctcggactg
     2761 gatttcgaaa ccgccgataa tccggagggt ggtcgttttt ccgcagccgc tggggccgag
     2821 catggtgaag aattcgttac gtttgatatg taacgttaca tccttaatgg cttcactgcc
     2881 attataatcc ttggaaacat gaagcaaact gatgagtgtt tccgccatct tcaatcctcc
     2941 gctgttgaat tccatgtaac taaaaacacc ggctcccgaa aaggtaagaa ggaaaaaatt
     3001 gaaaaaacac cgcttcttta accacgatcc gagaaccctt aaacgggcgt atgccttatc
     3061 cggcgtaatt ctccaaggtt gcttgtggat tcaggttagg aacattttgt tactataact
     3121 cgaaaaaccg taagaattta atcatgaaat gaagttttga cacaacctta ttttagagat
     3181 ttgaaatgat ttttcaaggg cgctttctat caaatctagt aaaaactcac aatattcctg
     3241 gctgagagtt ctcgggattt ccatttttaa agtttttcgt acctggagct tttcggggtt
     3301 gatcat
//
LOCUS       DASVMB010000534         9323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_142050,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000534 DASVMB010000000
VERSION     DASVMB010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142050"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..721)
                     /locus_tag="VF531_14990"
     CDS             complement(131..721)
                     /locus_tag="VF531_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715321.1"
                     /translation="MEHKAFLFDTRGFNKELRPLILTSGQTGDTQPLKIFITNHMGKA
                     RSPYTGELLEYDWENELENGDMQELADFGMTCYYLPEADQGLVYSWDALLEALRQIPI
                     EWDAEYCIFGRPLETASFMLDPGRIGTGFVQAEDISAIFRELAADKELLIAQGLPSSE
                     ETLYELTAAELTEAYDQLMSLYKDAMENECGLLMTF"
     gene            complement(1173..1877)
                     /locus_tag="VF531_14995"
     CDS             complement(1173..1877)
                     /locus_tag="VF531_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019420587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX7715322.1"
                     /translation="MKEFFEVENMDLFDEIKRIGKRPVIAQIGGFRPEEGLKSWFGGN
                     FVLKKDMNWPGDDDGLMVPVIQIVVDEVPNGKDFFGDTKLIQVYLNTKELPSTPAKNG
                     DGWRLLEYKSNDDMAVVETPKESQIHKPFQIKWILSEKPDFPCWEESWDYFDMTEINE
                     SEELSERFFDEFERYYRTKIGGYASYIQSLCGDYEYVFQISSEEKPGFMVGDNGNIYI
                     LKSKTDNQWFLHWDCY"
     gene            complement(1931..2326)
                     /locus_tag="VF531_15000"
     CDS             complement(1931..2326)
                     /locus_tag="VF531_15000"
                     /inference="COORDINATES: protein motif:HMM:NF025477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 22 family protein"
                     /protein_id="HEX7715323.1"
                     /translation="MEEQGYVSLWLGKCSTYEELDNYLSGSYTDDGDYIKSSFERDFK
                     IDRFDEDFREAFCDDFSAHDFEKLLLGCSYEKLVIPEFAKHFRLDSKEEFNAGLLLYD
                     FRYQGQVKEILSKGIYLKYIGSVEYDKIY"
     gene            complement(2360..3310)
                     /locus_tag="VF531_15005"
     CDS             complement(2360..3310)
                     /locus_tag="VF531_15005"
                     /inference="COORDINATES: protein motif:HMM:NF021532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2262 domain-containing protein"
                     /protein_id="HEX7715324.1"
                     /translation="MAYYDTDKMLDTLGLAYEGDEQARAAIQGILLPHAVSDDYSVLI
                     LVQEKYASINTQGLVRTLNMPPEENGGRKQMADFYHCDQESKSWSSFPLPDSFAVREM
                     EYADGVFSFIADDCSCASARVISGTSGQIGKATMESSFAVNGVSESVIEKCFLNIPTT
                     VVKPSQPKKTAAKNLPIRDEIIGMLTFNERFQWFETKYQTKGYSFELDIDIDNRAEVI
                     KKLPMVREIITHLESIDQKMRQFAVEQLLELKNETWREEGEPELTKADFTARMSIETI
                     SFNDDGEYEIWYGDGDLFWGHSITVSVDRKGRPERADING"
     gene            complement(3331..5055)
                     /locus_tag="VF531_15010"
     CDS             complement(3331..5055)
                     /locus_tag="VF531_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7715325.1"
                     /translation="MNFNEYLTQGDLDNVKKLIENGTDIETTDEDGMTPLMIAASNGH
                     LEIVKYLLEKGARLEATDELQMTPVMIAACYDQLEVVKYLFEKNTDIERKNKYGLTTA
                     MLAAYNNCRDVLEFLLNNGANVAAKDIDGWNALMLASKKGHAAIVEYLIDQGVDLEAE
                     DNQGNTALMLAVEKEQSEVASLLITKGANTGKFDIFSASANGLLERVQQLVSGKVNLQ
                     TKNDAGLTPLLLAVSNGRLEIVKFLLDNGSNLQETDDERMTPLMLAATNDNLAMVQYL
                     VEKGANLESCTAEGETALRLAADRWQIEILQYLIAQGADIDARDNRLRTALMSAAAGD
                     NLDIVRCLVEGGADPEATDEKGQTALMLAADECQADTVQYLLEKGADIGQLDIIRASM
                     VGLTGRVQQLLEAGVDRETRNDQGMTPLILAAKNGHPETVRYLVEKGAQVGAADVNGM
                     TPLIFAANCGYPEIVKYLIDNGADIERADNEGTTPIVFAIFNRQLEIVQFLVDKGASI
                     QVPDHFGSTPLMQAARFGHLEIADYLLNNGADLNARDQDGKTALDYATEYEQDTLVDY
                     LRSKMVKN"
     gene            complement(5293..5499)
                     /locus_tag="VF531_15015"
     CDS             complement(5293..5499)
                     /locus_tag="VF531_15015"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX7715326.1"
                     /translation="MGIDYQIENGPVFTTLRVKMAQGESFRAEAGAMIAMSPTIGMEA
                     KTVGKGIFGDLKATVGGESLFASL"
     gene            complement(5699..6571)
                     /locus_tag="VF531_15020"
     CDS             complement(5699..6571)
                     /locus_tag="VF531_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715327.1"
                     /translation="MKKGFFLLAALLCVTFLLIGCGGGPGGSNPGDNTGVSDSLNAQL
                     NARSQAASSLQQVAKNAEQAGQEKMQQTPPPGPSGMVAAFGNILAAEGEEPEGEEPGG
                     THGPKWTGPDADGWYSITGRMSGLFEDHVLKIQMKLPDDSTTMYKVEDTTTAKDGSPI
                     GVGSEETTYVKGADGLVSGTSTYIRYYRFPNAENGRMTDYKAIATQSFSNFSPNGTGH
                     YETTLNLISTDYPAQGGTIINDRKYKEILDINFNLDRTKLHASGTIQDVTTPGAEGPA
                     SPYEDDWELITGAN"
     gene            complement(7087..8115)
                     /locus_tag="VF531_15025"
     CDS             complement(7087..8115)
                     /locus_tag="VF531_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715328.1"
                     /translation="MNRRFLFLGCLVLLLCLSISVFAEGTPAKVTIAADGPVVDVCYS
                     VDGSKIVFAYNNVINLYKVDGTLIKRFGIKDGPDIDVMDYANNTIVTFSYTNPALINI
                     WDENGTNIKKLGAVVKNDGDEKSIPEIETAKSYRMLFSRDGKSIVNVSKTDNFGGVKA
                     RDLATGKVTVFKGYDDTSVYGCSFSADGSKFVTISRDEQVKVWDLAKGALITTIETGD
                     SLKSSYDGKDVAILGDGSIVVVKTDLTLWSGDGKLIKTLKKEIDSDYTLVRASRDGNL
                     FAVAFEESGLALYSKDGSLLKVFQTEGQYSLPTAVTGIAFSPDNKFMAVATDGGDMGK
                     GDVRIFGL"
     gene            complement(8441..>9323)
                     /locus_tag="VF531_15030"
     CDS             complement(8441..>9323)
                     /locus_tag="VF531_15030"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX7715329.1"
                     /translation="GKFAYVVNKNSNSVTAYSINADGSLTPINTDITNSSYPTGTYPS
                     FGIVDPTGKFIYVTNTTSDNITAFSIDPVTGALESIGGSPFATSRDPNTVTIDRNSKF
                     AYAANLLSNSISIFSIGTTGALSNKSDVSISVEGTQSPYPTSITITPDGKFAYVPLQG
                     STKIVAYAIDAANGGLIEISGSPFTIGSASCSIAIDPTGKYAYVANLNTDDVTGYSID
                     VTTGALTPVSGGPFTAGNMPEDIAIDPTGKFVYVTNYYTNNVMAYSIDAVSGALKVIG
                     TSFATGKGPSRIVIVKK"
BASE COUNT         2472 a         2273 c         1812 g         2766 t
ORIGIN      
        1 gagagacagg tctgctcccc cgaaaaagta gcagtatcct tctttgcctg cattatgatt
       61 cgctcccaat cggtgaattc caggtcatta ataacgcaac ataaacattt taagccttat
      121 cacaatattt ctaaaatgtc atcaataacc cacattcatt ctccatggca tctttatata
      181 atgacattaa ttggtcatag gcctcagtca actccgcggc agttaattca tagagtgttt
      241 cttcactgga aggcaatcct tgggcaatca gaagttcttt atcagcagcc aattccctga
      301 aaatggcgga aatatcttca gcttgaacga atcctgttcc tatcctgccc ggatcaagca
      361 tgaaagaggc agtttctaat ggacgtccga agatgcaata ttccgcatcc cattcgatcg
      421 gtatctgtcg taatgcctct aaaagcgcat cccaagaata aacaagacct tggtcggctt
      481 cgggtaaata gtaacaagtc ataccaaagt cggctaattc ctgcatatcg ccattctcaa
      541 gttcattttc ccaatcatac tccagcaatt ccccggtata aggggaacgg gcttttccca
      601 tatgattcgt aataaatatc tttaaaggtt gcgtgtcgcc agtttgtccc gatgtaagta
      661 tcagtgggcg taattcttta ttaaaacccc gcgtatcgaa gagaaaggcc ttatgttcca
      721 ttggttaatt gccctttctt tatgattcag ccaaattaac ctatttccgg gccatccggt
      781 tcttttccga ctgacttaca acaattttag gattgtgtaa ataaagtcac atccaaaccc
      841 aaataatcgt cttccccatc ccggttggat tctatcatcc ttatgcttac catgatttga
      901 aatcctatcg ggtttatatg ttttatgtat gaaacacaat cgatataata attaccggtt
      961 agcctactac gcactgggaa taaattgtcc taatcagcaa agcataaatt atcatcattt
     1021 acatatttga tgatctcatg atgaacaatt aacagaattt catccagcgt tcagataatt
     1081 tttgaaaccc agttaaagtg attccgtcat aaaactttat gtgctatatt ctttttaatt
     1141 cactcgattc gcatattatc ttgaatatct cctcaataac aatcccaatg taaaaaccat
     1201 tggttgtccg tttttgattt tagaatgtag atattgccgt tgtctccgac cataaatccc
     1261 ggcttttcct cggaagatat ctgaaaaacg tattcataat ccccgcatag agactgaata
     1321 taagatgcat atcctcctat cttagtccgg taatatcttt caaactcatc aaaaaaacgc
     1381 tcgctaagtt cctcggactc attgatctcc gtcatatcaa aataatccca ggactcttcc
     1441 cagcaaggaa aatccggctt ctctgataga atccacttga tttgaaacgg tttatggatt
     1501 tgagattctt taggcgtttc cacaacagcc atatcatcgt ttgatttata ttcaagcagc
     1561 cgccagccgt caccgttttt agcgggtgtc gatggcaatt ctttcgtatt aaggtagact
     1621 tgaatcaact tggtatctcc aaagaaatct tttccgttag ggacttcgtc aaccacgatc
     1681 tgaatgaccg gcaccatcaa tccgtcatca tcgccaggcc aattcatatc tttttttaaa
     1741 acaaagtttc ccccgaacca actctttaga ccctcttcag gcctaaaacc gccgatttgc
     1801 gcaataaccg gtctttttcc aatgcgcttg atctcatcaa acaggtccat attctccacc
     1861 tcaaaaaatt ctttcacgat agccgaccca caaatgatca accagccgat attgtcgatt
     1921 cgatgacaaa ttaataaatt ttgtcatact ccactgatcc gatatatttt agatagatgc
     1981 cttttgacaa tatttcttta acttgaccct gatacctaaa atcgtatagt aataaaccgg
     2041 cattaaactc ttctttggaa tcgagccgga aatgttttgc aaattcaggt ataactaatt
     2101 tttcataaga acagcccaaa agaagttttt caaaatcatg agcggaaaaa tcgtcacaaa
     2161 aagcttcacg aaaatcctca tcaaaccggt caatcttaaa atccctttca aaactggact
     2221 ttatgtaatc tccatcatcc gtgtaacttc ccgaaagata attatccagt tcctcatagg
     2281 tcgaacattt tcccaaccat aatgagacgt atccctgttc ttccattccc atccctcttt
     2341 cgaatgttat ctctcctttt cacccgttga tatccgcccg ctccggtcgg cctttccgat
     2401 cgacggatac ggtgatacta tgcccccaga ataaatcgcc gtccccatac caaatctcat
     2461 attcgccatc atcgttaaaa gaaatggtct caatcgacat ccgggccgta aaatcagcct
     2521 tggtcagttc cggttcgcct tcctcgcgcc aagtttcatt cttcaactcc agcaattgct
     2581 ctacggcaaa ttgacgcatc ttttggtcga tgctttccag gtgggtaatg atctcccgta
     2641 ccatgggcaa tttttttatt acttcagccc ggttatcgat gtcgatatca agctcaaaag
     2701 aatacccttt agtctgatac tttgtttcaa accactggaa acgctcatta aaagtgagca
     2761 taccgatgat ctcatcccgg atgggcaggt ttttagcagc ggtcttcttg ggctgcgacg
     2821 gctttaccac agtcgtggga atgttcagga aacatttttc aatgacagat tcagacacac
     2881 catttacggc aaaagaactt tccattgtgg ctttgccgat ctgcccgctt gttccggaga
     2941 taactcgtgc gcttgcgcac gaacagtcat ctgcgataaa actgaacaca ccatccgcat
     3001 attccatttc ccgtaccgca aaactgtcgg gaagcggaaa actgctccat gacttgcttt
     3061 cttggtcaca atgataaaaa tcagccatct gcttgcggcc gccgttttct tcgggcggca
     3121 tattgagagt tctcaccaat ccttgagtat tgatcgaggc gtatttctcc tggaccaata
     3181 tcagcacgga atagtcatcc gacaccgcat ggggtagtaa gattccttga attgccgctc
     3241 tagcttgctc atcaccttca tatgcaaggc caagggtatc caacatttta tccgtatcgt
     3301 aataagccat ggggttgcct ccgctggcgg ttaattttta accattttac ttcgcaaata
     3361 atctacgagg gtatcctgtt catattccgt tgcataatcc aaggcggttt tcccgtcttg
     3421 atcccgcgcg ttaagatctg cgccgttatt cagcagataa tcggcgatct ccagatggcc
     3481 gaaacgggcc gcttgcatta agggggtgga tccgaaatga tcggggactt gaattgaagc
     3541 ccctttatcg accaggaatt ggacgatctc caactgacgg ttgaatatgg caaatacaat
     3601 aggggtcgtt ccctcgttat cggctcgttc gatatcggcg ccgttatcaa tcaaatattt
     3661 tacaatctcc gggtaaccgc aattggcggc aaagattaaa ggagtcatcc cgttgacatc
     3721 ggctgcgcca acctgagctc ccttttccac caagtatcgt acagtctccg gatgaccgtt
     3781 tttggccgcc agtattaacg gagtcattcc ctgatcgttt cgggtctccc ggtccactcc
     3841 ggcctcaagt aattgctgga cccgcccggt tagaccgacc atgcttgccc ggataatatc
     3901 cagttgcccg atatccgcgc ccttttccag cagatactga acagtatccg cttggcactc
     3961 gtccgccgcc aacattaatg cggtctgacc tttttcatcg gttgcctccg gatccgctcc
     4021 cccttccacc aaacaacgca cgatatccag attatcacct gcagcggcgc tcattaaagc
     4081 agtccgcagc ctgttatccc gtgcatcgat atccgcccct tgggcaataa ggtattgcaa
     4141 tatctcgatc tgccaacgat cggcagccag ccgcagcgca gtttcccctt ctgcggtaca
     4201 gctttccaaa ttcgcccctt tttccaccag gtactggacc attgccagat tgtcattggt
     4261 tgcggccaac atcagagggg tcattctttc atcatcggtt tcctgcaggt tggaaccgtt
     4321 atccaacaaa aattttacta tttccaaacg accgttgctc acggccagca gcagcggagt
     4381 caggccggca tcatttttag tttgaaggtt tacctttccg gagaccaact gttgcacccg
     4441 ttccaagagc ccattggcgc tcgccgaaaa aatatcaaat ttgccggtat tggcaccctt
     4501 ggtaatcaac aaactggcga cttcggattg ctctttctct actgccagca ttaaggcggt
     4561 gtttccctga ttgtcttccg cctcaagatc aacgccttga tcaattaaat attcgacgat
     4621 tgctgcatgg ccttttttgg atgccagcat caaagcattc caaccgtcga tatctttggc
     4681 tgcaacgttg gctccgttgt taagcaaaaa ctccagaacg tcccggcaat tgttataggc
     4741 ggccaacatg gctgtagtga gcccgtattt atttttccgc tcaatatctg tattcttttc
     4801 aaacagatat ttcaccacct caagctggtc gtaacaggct gcgatcatga cgggtgtcat
     4861 ctgcaactca tcggtggctt ccagcctagc gcccttttca agcagatatt taacgatctc
     4921 caaatgtccg ttactcgcag cgatcattag gggagtcatg ccgtcttcgt cggttgtttc
     4981 aatatccgtc ccgttttcaa tcaatttctt gacattatcc agatcgcctt gggtgagata
     5041 ttcgttaaaa ttcatattgc tctccttatg tcttgtattt gacaccgggt cactcttttt
     5101 ggtgtaagtt gttataatga tttccggagc tttcgcttcc aaatgatctc gcgcaaaaac
     5161 aaaagggcgt aactttacaa gactttatcc gctatcaacg cataaatctg taaagaaacg
     5221 ccctgatcac cctttccggc atccgtgtca aagaagagtt ttcttttata aagtcctcaa
     5281 tgatatacca cactataacg aggcgaacaa cgattcaccg cccaccgtgg ctttcaggtc
     5341 cccaaatatt ccttttccta cggtttttgc ctccatccca atggtagggg acatggcaat
     5401 catcgctccg gcctcggccc ggaaagattc tccttgggcc attttgacgc gtaacgtcgt
     5461 aaataccggc ccgttttcga tctgataatc aattcccacc gactgcctta tttgtaattt
     5521 ctaaggataa ttttgtataa ttgatctctt ttatttattt cgccaaaatt ttgttttttc
     5581 ttcttagcgt aaagggtgaa ttttttcacc ctattgtgaa gtgtggggac tcaatcaaaa
     5641 aagaccgtct ttttagaacg gtcttttttg gttgaactat tgattcattc gccagttttc
     5701 agtttgcccc agtaattaat tcccaatcat cctcgtatgg agaggcgggc ccttcggctc
     5761 caggtgtagt tacatcctgt attgtgccag atgcatgaag tttcgttcta tccagattaa
     5821 agttgatatc caggatttcc ttgtattttc gatcatttat gatagtacca ccctgtgcag
     5881 gatagtccgt agagatcaga ttgagtgtgg tttcataatg acctgtcccg ttggggctga
     5941 aattgctaaa actttgggta gcgatggcct tataatcggt catacggcca ttctctgcat
     6001 tcggaaagcg atagtatctt atataagtgg aagtaccgct tacaagcccg tccgctcctt
     6061 tgacataagt ggtttcttca gagccaacgc caataggaga tccatctttg gcggttgttg
     6121 tatcttccac cttatacatg gtggttgaat catccggaag tttcatctgg attttcaata
     6181 cgtgatcttc aaagagtcct gacatacgtc cagtgatact ataccagcca tcagcatcgg
     6241 ggccagtcca cttcggacca tgggtccctc caggttcttc gccttccggt tcttcacctt
     6301 cggctgctaa aatatttccg aaagctgcca ccatacccga agggccaggc ggaggggtct
     6361 gctgcatttt ttcttgtcca gcctgttcag cgttttttgc aacctgttga agtgaggaag
     6421 ctgcttgact gcgggcattg agttgggcgt taagactatc gctgacgcca gtattgtctc
     6481 caggattact gccgccggga ccgccgccgc atccaattaa aagaaatgtt acacagagta
     6541 aagctgccag tagaaaaaat ccttttttca ttaaaactcg acctcctatt cagaatttga
     6601 tagatcatct acaaaaatgg ttgtaaattc tggaaaatca cctcatcttt atagcaacaa
     6661 cattttgctt ttcatatccg tgaaaccaaa acttgtcatt caactttcat atatcgataa
     6721 ttaaacccaa aatcagttcc cggataatta tgatctttgt gtaaattatt tttttacgat
     6781 tatctactat caatatagtt cataatttta aaatgataaa gattgaacat tttcacctaa
     6841 aaataaaata aggggtgaaa atgttcaccc cttaataaaa attttcagat aattaaaaag
     6901 taacgcccat ctgtattcct aacaataaag ccgactcctt tttcgagccg gccggtcaaa
     6961 aagtctattt tggggggtta ttgggaacgt cctgcgaagc tattttcagc tagaacacat
     7021 ccggttccca tcctgcttgg ttcaagcatg aatgatgcat gaagatattt gaaagccccc
     7081 catattttag agtccaaaaa tcctgacatc ccctttaccc atatctcctc catcggttgc
     7141 aacagccatg aacttgttgt ccgggctgaa ggctataccg gtaaccgcgg tggggagtga
     7201 atattggcct tcagtctgga atactttcag caaggaaccg tctttgctgt ataaagccaa
     7261 tccgctttcc tcaaacgcca ctgcaaacaa attgccgtcg cggcttgcac ggacaagggt
     7321 ataatcggaa tcaatctctt ttttcagggt tttgataagt tttccatcgc cggaccaaag
     7381 tgttaggtcg gttttaacaa caacaatgga gccatcgcct aagatcgcaa cgtctttgcc
     7441 atcataactt gacttcaacg aatccccggt ttcaatggtg gtaattaaag cccctttggc
     7501 cagatcccat actttgactt gttcatctct ggaaatggta acaaatttgc tgccgtccgc
     7561 actaaagcta caaccgtaga ccgaggtatc atcgtaacct ttgaaaaccg tcaccttccc
     7621 ggtggcaaga tcccgtgctt taaccccgcc aaaattgtcg gttttcgata cattgacgat
     7681 acttttcccg tcacgggaga acaacatccg gtagctcttt gcagtttcaa tctcgggaat
     7741 tgatttctcg tcaccgtcat ttttaacaac cgcaccgagt ttcttgatat tcgtgccgtt
     7801 ttcatcccaa atattgatga gtgcgggatt tgtataggaa aatgtgacga tggtattatt
     7861 ggcgtagtcc atcacatcaa tgtccggacc gtctttaatt ccaaaccgtt taatcagcgt
     7921 cccgtcgact ttatacaaat taataacatt gttataagca aagacaattt tcgatccgtc
     7981 gacgctgtaa caaacatcga ctacagggcc atccgcagct atcgttacct tagccggagt
     8041 cccctcggca aataccgaaa tggataaaca aagcaataaa accaggcacc caaggaaaag
     8101 aaatcttctg ttcatttaat cgctccttag cagaaaatgt tatatcaaac cttaattata
     8161 atataatgcc tgacatcacc agttgaaaga tgattatttt caccccaaaa tatcaaaagg
     8221 ggtgaaagtt ttcacccttt tgatatttcc ggatcactta aggttttctt atacaataag
     8281 ccgactcgtt tttcgagccg gccggttaga gagttttgtt ttttggagtt attggaacgt
     8341 ccttcgcaaa tattttcagc ttgaacccaa tccagttccc atcctgatac cctatcaaaa
     8401 gctacgatcc attttaaccc actctgctat tttgttaagt ttattttttt actataacaa
     8461 tacgactggg acctttaccg gtggcaaacg aggtgccgat gactttcaat gcgccgctga
     8521 cggcgtcgat ggaataagcc atgacgttgt ttgtataata gtttgtcaca taaacgaatt
     8581 tgccggttgg atcgatagca atgtcctcag gcatattccc ggcagtaaaa gggccaccac
     8641 ttacaggcgt taacgcaccg gtcgtgacgt caatggaata gcccgtaaca tcatctgtat
     8701 tcagattagc gacataagca tatttgccgg tcgggtcgat ggcgattgaa catgaggcgc
     8761 taccaatggt aaagggactg ccactaatct ctattagtcc gccatttgcg gcatcgatag
     8821 cataagcgac aattttagtc gaaccctgaa gtgggacata tgcgaatttg ccgtctggag
     8881 tgatggttat tgaggtggga taaggagatt gtgtaccttc cacactaata gagacgtcac
     8941 ttttatttga caacgcaccg gttgtcccga ttgaaaaaat actgatactg ttggatagta
     9001 aatttgctgc atacgcaaat ttggaattcc ggtcgatagt cactgtatta ggatctctac
     9061 tggtggcaaa ggggctaccg ccgatagact ccaatgcgcc agtcacggga tcaatggaaa
     9121 aagctgtaat attatccgat gttgtattag tgacatagat gaatttcccg gttggatcaa
     9181 cgattccaaa acttggataa gttccagtgg gataggaact gttggtaatg tcggtattga
     9241 tcggtgtcaa tgacccgtcg gcgttaatcg aataagcagt gacactgttt gagtttttat
     9301 ttacaacata agcaaatttg ccg
//
LOCUS       DASVMB010000535        11125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_727099,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000535 DASVMB010000000
VERSION     DASVMB010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727099"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..500)
                     /locus_tag="VF531_15035"
     CDS             complement(84..500)
                     /locus_tag="VF531_15035"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX7715330.1"
                     /translation="MNCGQCYSGELTGYLYNSTGSMETAEDIASEVFVKALSYQSTLA
                     EMSQAQCRLWLYTSAKRKLIDLARKRKTESRVITTVDPVNQGPELIKSGFEAICKYTK
                     MNLIDVYGVCTSQYKHIKEDMVNLQKAYEMGLRISL"
     gene            complement(648..2165)
                     /locus_tag="VF531_15040"
     CDS             complement(648..2165)
                     /locus_tag="VF531_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7715331.1"
                     /translation="MDYFCFIQSVVAYVENRLKTDFDYAGLERATGFSLPHTREVFKT
                     CTRMPLARYVLYRKLSNAAFEMVHTERRMLDIAVDYGFESYDTFIRAFKRLTGVTPRE
                     FRRQCFQVGRIKLTAGIYGPGIAKNEKTVLTPQPNLEEMMTMQDVTMKTVRKSEDSCI
                     LFGVPKVQYCSEECTPFPSVLKACLNYMGQEINYSYLMAASGAAFRLRWNTTSWDGGN
                     VDIMCIYENPVEAFERSFKAAGRSFKILERRSEVTKEDFIHFIKSEIDEGRPVIALGI
                     IGPPEACIISGYRDYGSTLLGWNFFQDNPEFAKDSAIDESGYFVCNSWWQNSNTIGVM
                     AISEAKETGTDMKDILQNAIDIMTKERIGDYAGGPAAYDAWAGALSDESQFPQNAVLP
                     ILFERLMCQGDAMDMIGEGRAYAAGFIEWLATQGENIHPSIKTKCLEAAKYFKEEASI
                     PQGMCKILGGWQRGEEQARRLADPAVRSDIGRLITRAKELDTKAGCLLKGIVEEL"
     gene            complement(2265..2582)
                     /locus_tag="VF531_15045"
     CDS             complement(2265..2582)
                     /locus_tag="VF531_15045"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7715332.1"
                     /translation="MKQSEIFNQVYDIVAQIPGGKVMTYGQIAMMLGSPYYARRVGQA
                     MFNTPEYLDIPCHRVVNSTGGLAPAYAFGGEGKQRAVLEAEGVIFKENGCIDLKKCLW
                     RPD"
     gene            2845..3201
                     /locus_tag="VF531_15050"
     CDS             2845..3201
                     /locus_tag="VF531_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7715333.1"
                     /translation="MKFDTTIPIYLQIMNCIKKDIVTGTLPPGERMDSVRTLAEKFEV
                     NLNTMQRAASELEREEVITTQRGIGSFITGDEKTIAALKEEMSTKLVERFIGEMKGIG
                     YRKDEILEIIRKALEK"
     gene            3259..3888
                     /locus_tag="VF531_15055"
                     /pseudo
     CDS             3259..3888
                     /locus_tag="VF531_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3881..4606
                     /locus_tag="VF531_15060"
     CDS             3881..4606
                     /locus_tag="VF531_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715334.1"
                     /translation="MLNLLKWDLRNFIKTYDWLYTGFAICFVIALLPDSVPFFSLVTN
                     GLAAVYSVFFFGYTIVISAGYTISWLRNDSFQLELSLPVTPWKMLLSKLILATLINIS
                     GMVLSMVLWLMIDKFGMSNITLFRGFAGFLQYLLSMLTLLVIFMFSYMMAKSFRFTRK
                     KAGIATMIIFFTICSLVAACFLATGAWNITVGAGATFNILMNEKLRWLANLSAIIGPV
                     VVLAAGFSGSCVLFRQKFERYES"
     gene            4621..5475
                     /locus_tag="VF531_15065"
     CDS             4621..5475
                     /locus_tag="VF531_15065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7715335.1"
                     /translation="MKRFRTQVQTLAFQYPVIFSFIIMTLGIVLTEIHLENYLKSYID
                     FQSASYLTGIFEQGLCSIILAALISGLGLLKSAGFTRPKEWKQLWLIWPVIVLSILNG
                     WSLFTGTIVIDTSKPTLILLFMLLYLSTGFFEEFLFRGVVTTVMLQKWGGTRKGIYLA
                     VIVSSGLFGVLHIINFFMNRFTLLANFTQTIYALFFGVFFAACLLRNNSIWPVVGAHA
                     IFDICGNFNEIAFVGNFGQIHQTTWNEAFATVIITLPLFIYGLFILRKVKPSTQSKVN
                     EAGFTVSY"
     gene            complement(5777..7291)
                     /locus_tag="VF531_15070"
     CDS             complement(5777..7291)
                     /locus_tag="VF531_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX7715336.1"
                     /translation="MNSNVLQIRPEDNVAIALASLTKGDVIEVGKDRIEILNDIPQGH
                     KVALRDVPVGSQIIRYGYPIGRATIDIRRGQWVHSHNLGTGLTERLEYSFQPDLSGWK
                     GVHDGAVKHMFQGYRRDDGRVGIRNEIWLINTVGCINKQTEKLAALARERFRSEIEPG
                     KIDGIFAFSHPYGCSQLGEDLVNTQKILAGLVNHPHAAGVLVLGLGCENNQMAEFQKF
                     IGTQNPERIKFLNFQDVSDEIETGLELIAELVRYASGLQPVTLTLSDLTIALKCGGSD
                     GFSGITANPLVGRISDLIIGYGGTSILSEVPEMFGAETILMNRAKDRETFEGIVRLIN
                     GFKEYFVRNGQVVYENPSPGNKAGGITTLEEKSLGCTQKGGTSPVVDVLHYGDRVKVK
                     GLNLLEGPGNDIVSTTALTAAGANLILFTSGRGTPLGAPVPTLKIATNTPIFQKKPGW
                     FDFDAGRLLTGAGLDDLAEELLGQIVEVAGKKRPSRNEINDYRDIAIFKNGVTL"
     gene            complement(7367..8893)
                     /locus_tag="VF531_15075"
     CDS             complement(7367..8893)
                     /locus_tag="VF531_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HEX7715337.1"
                     /translation="MESFIGFIAEGQTVADLKATVANRSEYASIYPDSIHQVDQTVLF
                     MMKGEHVKKLCIVGDSSPIFSEIAGEIVNTIPGPKVKVADLSVENSKVIRKYFDFANP
                     KAMSGAAATIGLGDRLGLASPGHIRLIKQTKMHPILAQQSIRELNLTGRNYAGVMADA
                     VWAVFQEDYRGGFGADGDHLKTPAEVGMALDNGYTMITLDCSEHINNVSVNDKTAIDQ
                     ILAKIDPAQIKKLEQTFLGHEIKLQTGLALAFTPEKLKLNIAVYQQAVNFAIQIYSEF
                     LKPLAGKVDFEVSIDETMTPTDPASHYFVAEQLTAAAVKINSMAPRFCGEFQKGIDYI
                     GDLKQFAREYGEHDKIARHYGYKLSIHSGSDKFSVFPTIGKVSEGYIHVKTAGTNWLE
                     AVRVIIRTDPALYREMHKFALENLMEAKKYYHITADMNRVPNIDTLSDAQLADLMNQN
                     DARQLIHIAYGLILTAKDKAGNSRFRDRIYSCLYQNEELYYEMLQNHIGKHIKLLGLM
                     "
     gene            complement(9021..10559)
                     /locus_tag="VF531_15080"
     CDS             complement(9021..10559)
                     /locus_tag="VF531_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEX7715338.1"
                     /translation="MAALDSLNRTLLEEKLLGTTLADYPRELLNYPERVIQFGEGNFL
                     RAFVEWMIHEMNKAGAFKGRVVVVQPIAQGRVSDLNAQDGLYTLLLRGVQNGKVVNSR
                     EIIPAISRGLDAYSQWREVLKCAEDPQIEFVVSNTTEAGIVYLPGEKLDDTPTCANPP
                     KSYPAKLAVYLYHRFQFFHGDPAKGMVIIPCELIEANGSNLKAVILKLAAEWGLPPEF
                     SQWIEAHNYFLNTLVDRIVTGYPSKDAAKIESELGYHDQNLDTGEIFHLWVIEGDARL
                     ADRLPFHKAGLNVKWVKDLTPYRTRKVRILNGAHTSTVAVACLSDIDIVRDAVNDGTV
                     GKFMQSVLFDEIIPTLDSDVNELKGFAKDVLERFNNPFIDHKWFDIALNSTSKFETRV
                     MPSLRRYVEKSGKLPKKLTFSLAALIALYHGTEIRGNKLVGSRNGKEYFIQDDPETLE
                     FFRDNWNRYETGAITLAAIVDNVMHRFWPDFAKQHPQIITEVTNYLSVIVNSGIGPGL
                     KKCS"
     gene            complement(10598..>11125)
                     /locus_tag="VF531_15085"
     CDS             complement(10598..>11125)
                     /locus_tag="VF531_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7715339.1"
                     /translation="LNYRKNLWSTEKAGKVMGELMPYVNLCIANEEDAEKVFGIKAAD
                     TDVTGGKLNEAGYKTVAQKLQEKFGFKQVAITLRTSISASDNLWAAMYYDGKEFFFSR
                     SYPVHIVDRVGGGDSFGAGLIYALLNGYSSRDVVEFATAASCLKHSIEGDFNQVTLAE
                     VTTLMKGDASGRVQR"
BASE COUNT         2825 a         2787 c         2488 g         3025 t
ORIGIN      
        1 cagctatttc atcaagcttt tcaagcaaat caagggctgc actccatcgg ccttcaggaa
       61 aaatcattag gtttatcacc gattcataac gaaattctca accccatttc ataggccttt
      121 tgaaggttta ccatatcctc cttaatatgc ttgtattggc ttgtacatac cccatataca
      181 tcgatcaaat tcatcttggt atatttacat attgcttcaa agccagactt aataagctcc
      241 ggaccctggt taaccggatc gactgtggtg ataacccggg attccgtttt tctcttgcgg
      301 gcaagatcga tcaatttacg tttagccgag gtatagagcc ataaacggca ttgcgcctgt
      361 gacatttcgg ccagtgtact ctggtaactc aaagccttca caaatacttc cgaggcaata
      421 tcctcggccg tctccatgct acccgtggaa ttatacaaat aaccggtaag ttccccacta
      481 taacattggc cacagttcaa ttagatccat gggttaaccc ttcttcctca cgcttccata
      541 tattaaaccg gatgatttga agaaagtgtt ttcatttttt tctaaatata aaaaaccgga
      601 tgctccttcc acccggtttt ccactccaac aatctctttc gctaaggcta cagttcttct
      661 acaatacctt tcagtaggca tcctgccttg gtatcgagtt ccttggctct cgtaatcaga
      721 cggccgatat cgcttcgaac cgcaggatcg gccaaccttc tggcctgttc ctccccgcgt
      781 tgccacccgc ccaatatttt acacattcct tgcgggatgc ttgcctcctc cttaaaatat
      841 ttggccgcct cgaggcattt cgttttgatt gagggatgta tgttttcccc ctgtgtagct
      901 aaccattcta tgaaaccggc cgcataggcc ctgccctcac cgatcatatc catggcatcc
      961 ccctggcaca taagccgttc aaagagtata gggagtacgg cgttctgagg gaattgactt
     1021 tcatcggaga gtgccccggc ccaggcatcg taggcagcag gaccaccggc atagtcgccg
     1081 atccgttcct ttgtcattat atcgatggca ttttgcaaga tatccttcat gtcggtaccg
     1141 gtttctttgg cttcactaat ggccatgacc ccaatggtgt tggaattctg ccaccagctg
     1201 ttacaaacga agtagccgga ttcatcgatc gcgctgtcct tggcgaactc cgggttatct
     1261 tgaaaaaagt tccaccccag caaagtgctg ccgtaatcgc ggtatccgct aatgatgcaa
     1321 gcttcggggg gaccgataat tcccagcgct atcactgggc ggccttcgtc aatctccgac
     1381 ttgataaaat gaataaaatc ctctttcgtt acttccgacc ggcgttcgag gattttaaaa
     1441 gaacgaccgg ctgccttgaa agaacgttca aaggcctcaa ccggattttc gtagatacac
     1501 atgatatcca cattgccccc atcccagctg gtggtattcc aacgtaaacg aaaggccgcg
     1561 cccgatgctg ccatcagata gctgtagttt atctcttgcc ccatatagtt aaggcaggct
     1621 ttaagtactg agggaaaagg ggtacattct tcggaacaat actgtacttt aggtactcca
     1681 aaaaggatac aagaatcttc agatttcctt acagttttca tcgtaacatc ttgcatcgtc
     1741 atcatctcct ccagatttgg ctggggggta aggactgttt tttcattttt agcgataccc
     1801 gggccataga tcccggctgt cagcttgatt ctgcctactt gaaaacattg tcgtcgaaac
     1861 tcgcggggag tgactccggt taaccgttta aaagcccgga taaaggtatc ataactttca
     1921 aaaccgtaat ctacggcaat atccaacatc cgtctctcgg tatgaaccat ctcgaaggcg
     1981 gcattggata gcttgcggta aagtacataa cgggccagcg gcattcgggt gcaagtttta
     2041 aacacctcgc gggtatgggg caacgaaaac ccggtggctc gctccaggcc ggcatagtca
     2101 aaatcggtct ttagccggtt ttcaacatag gccaccactg actggatgaa acaaaaataa
     2161 tccatcccac accacccatg aattttcttc cggaagatag aaccattata cttaacccta
     2221 cagtcgaata ctcgactttt ttgaggatct attccggcga tttcttaatc cggccgccac
     2281 agacattttt tcagatcgat acagccgttt tccttaaaaa taactccctc cgcttcaaga
     2341 accgcccgtt gttttccctc gccgccgaaa gcataggccg gagcaagccc tcccgtactg
     2401 tttaccaccc gatgacacgg gatatccaga tactcagggg tattgaacat ggcctgacct
     2461 acccgccgcg cgtaataggg tgaaccgagc atcatggcga tctgcccgta ggtcatcacc
     2521 ttgccgccgg gaatttgggc tacaatatcg tatacctgat tgaaaatttc actttgtttc
     2581 ataaaagaag acccccttgc tttcaggaca agtgtccgat ccttcatcac caggtgttac
     2641 catatcatca tgaatatatc tagattatac tctctttggc gttcttcctt ccatcgatgt
     2701 tcattcatga attatttgag acccaatgaa aaccaactgg ggttttccga tgacaaaaaa
     2761 atgattgttc atggaaaact cttgacaatt ttctgtatta gtgtattata tacatataac
     2821 actaatacgg aagtgatcaa actcatgaaa ttcgatacca ccatccctat ttacttgcag
     2881 atcatgaact gcatcaaaaa agatattgtg accggaacgc ttccccccgg cgaaagaatg
     2941 gattcggtaa ggacattggc cgagaaattc gaggtcaatt tgaacacgat gcaacgggcc
     3001 gcttccgaac ttgaacggga agaagtcatt accacgcaaa gggggattgg aagctttata
     3061 accggagatg aaaaaacgat agccgcattg aaagaggaaa tgtcaaccaa actggtggag
     3121 cgctttattg gagagatgaa gggcattggc tatcgtaaag atgaaatcct ggaaattatc
     3181 agaaaggcgt tggaaaaatg agtgcaacgg tatcagtcag aaacttaacg aaatgcatcg
     3241 gcggaaaagg gggtagattt tcaacttcaa gccggaagag tggtcgggct tttaggaccc
     3301 aacggatccg gaaaaaccac tctgataaaa accttaatga gcatctacca tccggataac
     3361 ggtgagattc aaatctgcgg tgaacctgcc ggtttcggga ccaggaggca tatctcctat
     3421 atgcccgatg tgaaccatct gtttcaatgg atgcgcgtcc acgatgggat aaattactac
     3481 cgggagatgt ttggcgattt taatatccat cgctgtcgag agctttgtac atctctcgga
     3541 atcaatgaaa atgagaagat caagggtctt tccaaaggga tgaaagagcg cgtgctgatc
     3601 atgttgacgt tttcccggaa ttcacgcctt taccttttgg atgaacccat tagcggcatt
     3661 gatcctgtag ccaagaatcg tatcataaaa acgattttcg ccggttcaaa cggcgagtgc
     3721 accatcctga tctccacgca tcaagtcaag gatgtggaaa ccctgctcga cgaggtattg
     3781 tttatcaacg acggcaggat tatcttttcg gagagtgcgg acaccatccg cggtgtgcgt
     3841 ggcgagtcca ttgaagaatg ctatctggag gtttttaaaa atgcttaacc tattgaaatg
     3901 ggatttaaga aatttcatca aaacctatga ttggctttat accggctttg ccatatgctt
     3961 tgtgattgcg ttgttacccg atagcgttcc atttttctcg ttagttacga atgggttggc
     4021 cgccgtctac agcgtattct tcttcggcta taccatcgtt atatcggcag gatataccat
     4081 aagctggttg agaaacgact ctttccaact tgagctctcg ttgccggtaa ctccatggaa
     4141 aatgctcttg agtaaactga tactcgcaac cttgatcaat atttccggaa tggtcttgtc
     4201 gatggtatta tggttaatga ttgacaaatt cggaatgtca aatatcacgc tattcagggg
     4261 cttcgccgga tttcttcaat atctcctaag catgctgaca ctgcttgtca tttttatgtt
     4321 ttcttatatg atggcgaaga gctttcgttt cacacgtaaa aaagcgggaa tcgccacgat
     4381 gataatattt tttacgatat gttctttagt ggcggcatgt tttctggcca caggcgcctg
     4441 gaatatcact gtgggcgcag gtgctacctt taatatccta atgaatgaaa agttaagatg
     4501 gctggcgaac ctatccgcta tcatcggccc ggttgtagtc cttgccgccg gtttctccgg
     4561 cagctgcgta ctattcaggc agaaattcga acgatatgaa tcatgaaaag gaggatcccc
     4621 atgaaacgat tccgcacaca ggtgcaaact cttgcatttc aatacccggt aattttttca
     4681 ttcattatca tgacactggg aattgttttg acagaaatcc atctcgaaaa ttatcttaaa
     4741 agctatattg attttcaaag cgcttcgtat cttacgggaa tattcgaaca aggtttatgc
     4801 agtattatct tagcagcact tatttccggt ttggggttat taaaaagcgc cggatttacc
     4861 cggcccaagg aatggaagca actatggcta atctggcccg tcattgttct ctctatactt
     4921 aacggttggt cgctgtttac cggaacgatt gtcattgata catcaaaacc gactctaatc
     4981 ctgctcttca tgcttcttta tttatcaacc ggtttttttg aagagtttct tttccggggt
     5041 gtagtaacca ccgtaatgct gcaaaaatgg gggggtaccc ggaaaggaat ttatttggcg
     5101 gtcattgttt caagtggctt attcggtgta ttacatatca tcaatttctt tatgaaccgc
     5161 tttaccttgt tggcaaactt tacccagacg atatatgccc ttttctttgg ggtatttttt
     5221 gccgcctgtc tgttgcggaa caactccatc tggcccgtgg tcggagccca tgcgattttt
     5281 gatatatgcg ggaattttaa tgaaattgct ttcgtcggaa acttcggtca aatccatcaa
     5341 acaacatgga atgaagcgtt cgccacagtt attattactt tgcccttgtt catatacggc
     5401 ctgtttatac tgcgtaaggt gaagccttcc actcaaagta aagtaaacga agcaggattc
     5461 acagtcagtt attagcaaat gattaaatat aaataagagg gaaatcaaat gaagtccata
     5521 aaacagttta ttgaaaaaac ccatgatcgt ttcaagacgc ctggccgcgc cttctcccgc
     5581 tactagaccg aaacggacgg ccagtactgt agtgataaat ttccctcccc catgtaaatg
     5641 aactttatta agtggctgct gcatggggga gggccgaccc ggatactgat gaaggaataa
     5701 agaccctatg tttgttccct gttgcctatt ccctgttgcc tgttgccccg cgcccaacga
     5761 gagcgatgcc tcacgcctaa agtgtaaccc cattcttgaa aatggcaata tcgcggtaat
     5821 cgttgatctc attccgggag ggccgtttct taccagccac ctccacaatt tgaccgagca
     5881 gttcctctgc cagatcatcc agtcccgccc cggtaagcaa ccggccggcg tcaaaatcga
     5941 accaacccgg tttcttttga aagatcggcg tattcgtagc gatctttaat gtcggaaccg
     6001 gcgcgcccag gggagttccg cggccggagg taaacaagat caggttggcg cctgccgctg
     6061 tcagggcagt ggtggagacg atatcattcc ccggcccctc caaaaggtta aggcctttca
     6121 ccttgacccg gtcaccataa tggaggacat ccaccaccgg actggtaccg cctttttggg
     6181 tgcaacccaa cgatttctcc tccagggttg tgatcccgcc cgccttattc cccggggaag
     6241 ggttttcata aaccacctgg ccgttccgga cgaagtattc cttaaacccg ttaatcaacc
     6301 taacaattcc ctcaaaagtc tcccgatcct tggcccgatt catcagtatc gtctcggccc
     6361 cgaacatctc cggaacctcg gagagaatac tagtccctcc gtaaccgatg atgagatcgg
     6421 atatcctgcc caccaaagga ttggcggtga taccggagaa gccgtcggaa ccgccgcatt
     6481 tcaaagcgat ggtcaaatcg gagagggtca gtgtaaccgg ttgcaacccg gaagcgtacc
     6541 ggaccagctc cgcaatgagt tcgagtcctg tttcaatctc atcactgaca tcctgaaagt
     6601 tcaaaaattt gatccgttcc ggattttggg tgccgataaa cttttgaaac tctgccatct
     6661 gattgttttc acaacccaat cccaatacca gtacgcccgc ggcgtgggga tggttgacca
     6721 gtccggccag gatcttttgg gtattcacca gatcctctcc caattgcgaa caaccgtagg
     6781 gatgggaaaa ggcaaagatt ccgtcgatct ttcccggctc aatctcggag cggaatcttt
     6841 ccctggcaag cgcagccagc ttttcagtct gtttattgat gcaaccgacg gtattgatca
     6901 accaaatctc attgcgaatc ccgaccctgc cgtcatcccg gcggtaaccc tgaaacatgt
     6961 gttttaccgc cccgtcgtga acccctttcc atccggatag atccggctga aagctatatt
     7021 caagccgctc cgtcagtccg gtgccaaggt tatggctatg cacccactgg ccacggcgaa
     7081 tatcaatcgt agcacggcca ataggataac cgtaacggat gatttgcgat ccgacaggga
     7141 catcccggag cgccacttta tggccttgcg gaatatcatt caaaatctca atccggtctt
     7201 tgccaacttc gatgacgtca cctttagtca atgaagccag ggcaatggcg acgttatctt
     7261 ccggcctgat ttgaagtacg ttgctattca ttttcatcac catcttttca atattaaaaa
     7321 aacggaactc accatttcag ccggtgagtt cctttaagtc tttaaactac atcaaaccta
     7381 aaagtttaat gtgcttgccg atatggttct gcagcatctc ataatataat tcttcatttt
     7441 gatagaggca ggagtaaatt cgatctctga accggctgtt accggcttta tcctttgccg
     7501 tcaggattaa accgtaagcg atatggatca gctggcgggc atcgttttga ttcatcagat
     7561 cggccaattg cgcgtctgaa agcgtatcaa tattcggcac ccggttcata tctgcggtaa
     7621 tgtgataata ttttttcgct tccattaggt tttccagggc aaatttgtgc atttcccggt
     7681 acaatgcggg atcagtccgg ataatcacgc ggacggcctc aagccagttg gttccggcag
     7741 ttttcacatg gatatagcct tcacttactt tgccgatggt cgggaagaca ctgaatttat
     7801 cgctgcccga gtggatgctc aatttataac cgtaatggcg ggcgatttta tcatgttccc
     7861 catattctct tgcaaattgc ttcaagtcgc cgatataatc gatgcccttc tggaactcgc
     7921 cgcaaaaacg cggggccata ctgttgattt taacagcggc ggcagtcaac tgctccgcca
     7981 cgaaatagtg ggatgccgga tcggtcggag tcatggtttc atcaattgag acttcaaagt
     8041 cgaccttgcc ggccagcggt tttaaaaatt cgctgtaaat ttgaatcgcg aaattgaccg
     8101 cctgttggta aacagcaata tttaatttga gtttttccgg ggtaaaggca agagccagac
     8161 cggtttggag tttgatttca tggcccagaa aagtctgctc aagttttttt atttgcgccg
     8221 gatcgatttt ggctaaaatt tggtcaatcg ctgtcttatc gttcaccgat acattgttga
     8281 tatgctccga gcagtccaga gtaatcatgg tataaccgtt atcaagcgcc attccgactt
     8341 cagccggcgt tttcagatgg tcaccatccg ctccgaaacc accacgataa tcttcctgaa
     8401 aaacggccca tacagcgtcg gccataaccc cggcataatt gcggccggtt aaattaagct
     8461 cgcgaatgga ttgttgcgcc aatatcggat gcatctttgt ctgttttatc aagcggatat
     8521 gtcccgggga agcgagaccc aaacggtcac ccaacccaat cgtcgccgct gccccggaca
     8581 tagccttcgg attggcaaag tcgaaatatt tccggatgac tttgctgttt tctaccgaca
     8641 agtcggcgac tttaacttta gggccgggta tggtattaac aatttcaccg gcaatttccg
     8701 aaaaaatggg cgagctgtct ccaacgatac aaagtttttt aacatgctcg cctttcatca
     8761 tgaatagcac cgtttgatca acttgatgaa tggagtccgg atagatcgat gcatactccg
     8821 atctgtttgc aaccgttgct tttaaatcgg caactgtttg tccttccgca atgaaaccga
     8881 taaatgattc catctttttc acggtcacac cctccttttc ttcacatcta ttatattcca
     8941 ttcaagtaat tttttgttat aaagaggggg tttaatgaat attcaagttg attttaataa
     9001 aatacctgct atataagatt ttacgaacat ttctttaatc ccggcccgat cccggaattt
     9061 acgattacgc taagataatt ggttacttcg gttataatct gaggatgctg tttggcaaaa
     9121 tccggccaga agcgatgcat cacattgtca acgatcgcgg ccagggtgat ggcgcctgtt
     9181 tcgtaccggt tccagttatc acggaaaaat tccaaggttt ccgggtcatc ctgaatgaaa
     9241 tactctttac cattcctgga accgaccagt ttattgccgc gaatttcagt gccgtgatag
     9301 agcgcaatca aggcggccaa tgagaatgtc agtttcttcg gcaacttacc gctcttttca
     9361 acataacgac ggagtgaagg cattacccgg gtttcaaatt tggaggttga gtttaaagcg
     9421 atatcaaacc atttatgatc aataaacggg ttgttgaagc gttccagaac atccttggca
     9481 aatcctttca actcgttgac gtccgagtcc agagtcggga taatttcatc aaataataca
     9541 ctttgcatga acttgccgac ggttccatca ttgacggcgt cccggacgat atcgatgtcg
     9601 gatagacatg ctaccgctac agtagaggta tgcgcgccat ttaagatccg gactttacgg
     9661 gtacgataag gggtaagatc tttaacccat ttaacattca agccggcttt atggaagggc
     9721 aaacgatccg ctaagcgggc atctccttcg atgacccata aatgaaagat ctcgccggta
     9781 tcaaggtttt gatcgtggta tccaagttca ctctcgatct tggctgcatc ttttgaagga
     9841 tagccggtaa cgatccgatc gaccaacgta ttcaggaagt agttatgagc ctcaatccat
     9901 tggctgaatt ccggcggcag tccccattcg gctgccagtt tcaaaatcac cgcctttaaa
     9961 ttagacccgt tggcttcaat cagctcacag gggatgatca ccatcccctt cgcgggatcc
    10021 ccatggaaaa actggaaacg gtgatagaga taaaccgcca gtttggccgg gtaagacttg
    10081 ggtggattcg cgcaggtggg cgtgtcatca agtttctcac ccggcaaata cacgattcca
    10141 gcctcggtgg tattggatac gacaaattca atctgcggat cctcggcgca tttcaaaacc
    10201 tcgcgccact gactataggc atccaggccc cggctgatgg cgggaatgat ttcccggcta
    10261 ttaacgacct taccgttctg aactccccgg agcaacaggg tatataatcc gtcctgagca
    10321 ttgagatcgc ttaccctgcc ctgggcaatc ggctgaacca ctaccacccg gcctttgaat
    10381 gcccctgcct tattcatctc atggatcatc cattccacga aagcacggag aaagttaccc
    10441 tcgccgaact ggatcacccg ctcgggatag ttcaggagtt cccggggata atcggcaagg
    10501 gtggttccga gtaacttctc ctcaagcagg gtccggttga gactatctaa agctgccaag
    10561 tgagacactc ctttgatcaa tttttcctaa aaacaactta ccgctgcacc ctacccgaag
    10621 cgtccccctt catcaaggtg gtcacctcgg ccagggtcac ctggttgaag tcgccctcga
    10681 tggagtgttt cagacaggag gccgcagtgg cgaactccac cacatcccgg gagctgtacc
    10741 cgttcagtaa ggcgtagatt agtccggcgc cgaaggaatc gccgccgccc acccggtcca
    10801 cgatatgtac cggatagctg cgagagaaga agaattcctt gccgtcataa tacatcgcgg
    10861 cccataggtt gtccgatgcc gaaatgctgg tccgcaaagt gatggccacc tgcttgaagc
    10921 cgaacttctc ctggagcttt tgggctacgg ttttgtaccc cgcttcgttc aacttgccgc
    10981 cggtgacgtc ggtatcggcc gccttgatcc cgaagacctt ctcggcgtcc tcctcgttgg
    11041 cgatgcacag attgacgtag ggcatcaatt cccccatgac cttgccggct ttctcggtgg
    11101 accagaggtt tttccggtaa ttcag
//
LOCUS       DASVMB010000536         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_727110,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000536 DASVMB010000000
VERSION     DASVMB010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727110"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..786
                     /locus_tag="VF531_15090"
     CDS             352..786
                     /locus_tag="VF531_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715340.1"
                     /translation="MIDQQNGVLIFENGFTISPSLTEELFLDSELGKAATKEVINVSW
                     RSYRIESQKISGETFAVVLYFHREVLQSVHLANMDERFGISWSDWSEEKEMSRNRSHE
                     SWLKKIIGMQRIFGWGYVWSGYDSKAGSSSIVINYKNSSPSK"
     gene            833..1396
                     /locus_tag="VF531_15095"
     CDS             833..1396
                     /locus_tag="VF531_15095"
                     /inference="COORDINATES: protein motif:HMM:NF020461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity protein Tdi1 domain-containing
                     protein"
                     /protein_id="HEX7715341.1"
                     /translation="MELQAFIEKHRPEQDLQKVNQSKIDEYSTRLPACILELWQDYGF
                     GSYSAGLIRLIDPEIFNEILYTWLGKKNDKRIPIALSAFGELFYYRDLDNGAEDVSIL
                     KVHYKSIDVCTWSLKDFFNDYLCDDEIIEEVLRQELFRKALEKYGPLKDDEIYSFVPA
                     LVMGGAAEVEFIKKANALVHLDILFQM"
     gene            1556..2029
                     /locus_tag="VF531_15100"
     CDS             1556..2029
                     /locus_tag="VF531_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7715342.1"
                     /translation="MGTLIYDAAVAGDLEQVQAVVARSPEAVNEKDEYGFTALHGLAG
                     EEHYDIVRFLIEHGADVNAQNEDGITPLHLAAWPEMAEFLITSGADLEARTHGGDTPL
                     MVMAAETESEDVMEVLLEAGADVNARNNQGRTAYDIAKAREETDKMKLLKRYGHQ"
     gene            2073..2252
                     /locus_tag="VF531_15105"
     CDS             2073..2252
                     /locus_tag="VF531_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715343.1"
                     /translation="MDDKQIINEMFTAIEENDVESVTAVIDSDANILNMIVKLLIESG
                     IDTKIKYVTSRRKCG"
     gene            2534..2911
                     /locus_tag="VF531_15110"
     CDS             2534..2911
                     /locus_tag="VF531_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm1 family immunity protein"
                     /protein_id="HEX7715344.1"
                     /translation="MKVVYFDAETDCESIEALNRILASRDENGFNEFELYGSEKCPYL
                     SILVNDNLACVHFFEFEDDCGHYAYCDKELLVEDGYTTFYIGSTETEISNDLVIPFSL
                     VQVAAQDFFLTSKMSEKLKWFEL"
     gene            3051..>3628
                     /locus_tag="VF531_15115"
     CDS             3051..>3628
                     /locus_tag="VF531_15115"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX7715345.1"
                     /translation="MDKIYVVNQAAHLPDTSQIETLETAVGSALPKGYQEFIMEYGGG
                     ELNTFLYLLAPEEVLKATAEWHETLVCDFWDEDAGLSQDLRSQAYVLAYSIDSDLFVF
                     HAEKEGIFCLPRQDNQIYPVGKSLSEMIQWVFRAGKLIQAGDGLFYKPDGPTSMVRYR
                     NPNPPSLDSFMEAVKVLGPPDYSIRSEEFIDI"
BASE COUNT         1100 a          623 c          931 g          974 t
ORIGIN      
        1 atgaattaat attagcagca tataccgatg ttgctgatat tcaaaaatca aatcggaaga
       61 aaaattagtt ggatggtaaa aatacgatca atatattgca attgatttag aacttttcag
      121 atggaaatgg acttaaggaa aggcaagatt gattcaggtg agaagatcac tgtaaccaaa
      181 gataaaaagg ttttgattgc ggatggaaaa ttcaaacagg aattactgtt aatttctaaa
      241 tctatatgat gccggggcac gttgtatcct ttagatacga tgcaccggca acatcatcat
      301 gcgtacttaa gtgagataga attggagagc gatctgggag gtttgaagtc gatgatcgac
      361 caacaaaacg gggtattgat ttttgaaaat ggctttacca tctcaccctc gctgactgaa
      421 gaattatttc tggactcgga gttaggaaag gcggccacga aagaggttat aaatgtctcg
      481 tggcgctctt accggattga atcgcaaaaa atttccggag aaacttttgc ggttgtttta
      541 tacttccatc gcgaggtttt gcaaagcgtt catttggcca atatggatga aagattcggc
      601 atttcatgga gtgattggtc ggaagagaaa gaaatgagcc ggaaccggag ccatgaaagc
      661 tggctaaaga agatcatcgg gatgcaacgt atttttgggt ggggatatgt atggtcgggt
      721 tatgacagta aagcgggaag tagttctatt gttataaatt acaagaattc atcgccaagt
      781 aaatgatgag aggttactgt tccatattcg aaacttgagg aggactcacg ctatggagtt
      841 acaggctttt atcgaaaagc atcgaccgga gcaagactta cagaaagtaa atcaatccaa
      901 gatagatgaa tattccaccc ggcttcccgc gtgtatcctg gaattatggc aggattacgg
      961 ttttggctct tattcggccg gattaatccg gctgattgac ccggaaattt ttaatgagat
     1021 cctctatacc tggctcggaa aaaagaatga caagcgcatc cccatcgcgc tttcggcctt
     1081 tggcgagctt ttttattacc gtgatttaga taacggcgcg gaggatgtca gcatccttaa
     1141 ggtccattat aaatcgattg atgtttgtac ttggagtctc aaggacttct ttaatgatta
     1201 tttatgtgat gatgaaatta ttgaagaagt tctacggcaa gaactttttc gaaaagctct
     1261 tgaaaagtac gggcccttga aagacgacga gatctattcg tttgttccgg cgctggtaat
     1321 gggcggagcc gccgaagtag aattcatcaa aaaggcaaat gccctggttc atttggatat
     1381 tttgtttcag atgtaaaacc ttcaaaggat cacattggaa tcgactaaaa ttttagtggc
     1441 aatccctccc aagaatctgg actcatccca gaagtataaa gtaatcggat cggataaaga
     1501 aaatgtcact attgaatttg aatatgattc aagcctaaag gacaggaggt aactcatggg
     1561 aacattaatt tacgatgcgg cggtggccgg ggatttggaa caggttcagg cggtagtagc
     1621 ccggagccct gaggctgtta acgaaaagga cgaatacggt tttactgcgc tccacggcct
     1681 ggcgggagag gagcattatg atatcgtccg attcctgatc gaacatggcg cggatgtgaa
     1741 tgcccaaaat gaagatggga taacgccgct tcatttggcg gcctggccgg agatggccga
     1801 gttcttgatt acaagcggcg ccgacttgga ggcccgtacc catggaggag atactccgtt
     1861 gatggtcatg gcagccgaaa cggagagtga agatgtgatg gaggtgcttc tcgaagccgg
     1921 cgccgatgtg aatgcccgga ataatcaggg ccggaccgct tacgatattg ccaaagccag
     1981 agaagagaca gataagatga aattgttgaa acggtatggt catcagtgaa gtgagagacg
     2041 gaagtacaga tcttcacgaa agggttcaat atatggatga taaacaaata ataaatgaaa
     2101 tgtttactgc cattgaagaa aacgatgttg aaagcgtgac ggccgtaatt gattcagatg
     2161 ccaacatttt aaatatgatt gtcaagcttt tgattgagag cggcattgat acgaaaatta
     2221 agtatgttac atcaagaaga aagtgcggat gacctggaaa aagtgcgaaa acggcagatg
     2281 acagggacag agtttatact caaaagatgt gacggcaaac tctgggccga ggatttgagt
     2341 gaggaaggtt tagcttttat caggagctat tggctttagg catagctgac ggaccttata
     2401 ctttggagga cggttgggag aattaccaag agttcgctca aatgctagat gaagcttatg
     2461 aaaattggaa aaaagggcaa gcgggtagag caaggtagga aatatagctt ttggcctatg
     2521 gaggcagatg gacttgaaag tagtttattt tgacgctgaa accgattgcg aaagtataga
     2581 agcgttaaac cgcattttag ctagccggga tgaaaatgga tttaatgaat ttgaacttta
     2641 cggcagcgaa aaatgtcctt atttgtccat tttagtaaat gacaatttgg cctgcgtgca
     2701 tttttttgaa tttgaagatg attgcgggca ttatgcatat tgcgataaag aattgttagt
     2761 tgaggatggt tatacaacct tttacattgg ctcaactgaa actgaaatat caaatgattt
     2821 ggtaatccca ttttctttag tgcaagtggc agcgcaagat ttctttttaa cctcgaaaat
     2881 gtccgaaaaa ttgaaatggt ttgagctatg atatgtacct tccaaacaag gagcgttgaa
     2941 aaatgtggaa acagcaaata gctgaaatta aagcaagacg ggaacggcgg ggtatagtta
     3001 ttgaagacta tagcaacgaa taagacccaa attacagaga gggttacact atggataaga
     3061 tctacgttgt aaaccaagcg gcccatttgc cggatacatc tcagattgaa acgttggaaa
     3121 ctgccgtcgg atcagctcta ccaaagggat atcaggaatt catcatggaa tacggtggcg
     3181 gggaactgaa tactttcctg tatctattgg caccggagga agtacttaag gctacagccg
     3241 aatggcatga gactttagtc tgtgattttt gggatgaaga tgccggttta tcccaagatt
     3301 tgcgtagcca agcctatgta ctggcttatt ctatcgattc ggatttattt gtctttcatg
     3361 cggaaaaaga ggggattttc tgcctgccgc gacaggataa tcagatttat ccggtgggga
     3421 agtcactttc ggagatgata cagtgggtgt ttcgagccgg gaaactaatc caagccggtg
     3481 atggtctgtt ttataaaccg gacgggccta ccagtatggt gcgttacaga aatcctaatc
     3541 cacccagttt ggattcgttc atggaagctg tgaaagttct cggcccgccg gactattcga
     3601 ttcgttcaga ggagtttatc gatatatt
//
LOCUS       DASVMB010000537         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_506212,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000537 DASVMB010000000
VERSION     DASVMB010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506212"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /gene="hypF"
                     /locus_tag="VF531_15120"
     CDS             complement(<1..1486)
                     /gene="hypF"
                     /locus_tag="VF531_15120"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX7715346.1"
                     /translation="MVENELTRLRLMVSGLVQGVGFRPFLYNLARELSLTGWVRNNFA
                     GVELEIQGTPEALTAFREALSSRAPSAAVIDRIDLETVPVLTESGFTILSSEAGQLEG
                     SPLPDQGICPACIAEFQNPHDRRYKYAFNSCTICGPRFTIMEALPFDRVRTSMERFEL
                     CPACRDEYTNPADRRFHAQTIACSDCGPELWFTTPAGERLNGDPAVQAGELLKSGAII
                     GIKGIGGYHLACDARNSQAVARLRQLKGRDNRAFAVMFRDIDALQDHCEVSSEELRAL
                     TGPARPIVLLLQKSSSSLSREVNPGLKELGAFLPYTGIQLLLFDATITALVMTSGNRS
                     GEPLSTDNAEAVRDLGPMVAGFLSHNRPILWRCDDTVLRVAVGRVVGIRRSRGFVPAP
                     FQGGRSLPPLLACGPQQKNTFALTKGERIYLSQHQGDLDNLPAYLGYQETITRLERLL
                     NCKPEWVVHDLHPDYNSTIYARNTGLPAIGVQHHLAHLAGVTFAG"
     gene            complement(1576..2094)
                     /locus_tag="VF531_15125"
     CDS             complement(1576..2094)
                     /locus_tag="VF531_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715347.1"
                     /translation="MRTTSKTINSIAVIFKIIAGFLFLMGLGGILTDQLLRMLVQGFP
                     TAEMYGDSWFLKLILQNFTLLAVLQILISLLLYLTCRGFLRREDWALGGLRFFSWFFL
                     AFNIMAGICFITQAIPDTLGASADTFKAFIGSSAGLLCMIFLILIRRLNSPGLRQEFG
                     ERGTGSNLVPRD"
     gene            complement(2307..2528)
                     /locus_tag="VF531_15130"
     CDS             complement(2307..2528)
                     /locus_tag="VF531_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX7715348.1"
                     /translation="MVQPGRYRHFKGREYQVLGTANHSETLEPYVVYKALYGEGQLWI
                     RPEALFMGTVRVEGRDVPRFEYLDSGDPT"
BASE COUNT          642 a          727 c          653 g          562 t
ORIGIN      
        1 cccccgcgaa tgtaacaccg gccagatgag ccaggtgatg ctgaaccccg atagccggca
       61 gtccggtatt tcgcgcgtat attgtggaat tataatccgg atggaggtcg tggaccaccc
      121 attccggctt acaattgagt aaacgttcca aacgggtaat ggtttcctgg taaccaagat
      181 aggctgggag attatccaga tcaccctggt gctggctgag atagatccgc tcccccttgg
      241 tcagcgcaaa ggtgtttttc tgctgtgggc cgcaagccag tagcggcggc agacttctgc
      301 cgccctgaaa cggcgcggga acgaacccgc gggaacggcg gattcccacc acccgcccaa
      361 ccgccacccg gagtaccgta tcatcacagc gccatagaat cggccggtta tgactgagaa
      421 atccggccac catcggaccc agatcccgta ctgcttcggc gttgtcagtg cttaacggct
      481 cgccggaacg gttgccgctg gtcattacca gtgcggtgat tgtggcatcg aagagtagca
      541 attggattcc ggtgtaaggc aggaaggcac ccaattcctt gagtcccgga ttgacctccc
      601 gggacaagga actggaggac ttctgcaaca agagcacaat gggtcgggcc ggaccggtca
      661 atgcccggag ttcttccgaa gaaacctcgc aatggtcctg taaggcgtct atatcccgga
      721 acatcaccgc aaaggcccgg ttatcccggc ctttcaattg gcgtagtctg gccacggctt
      781 gactgttccg ggcgtcacaa gccagatggt aaccaccgat acctttaatg ccgataatag
      841 ccccggattt taacagttct ccggcctgga ccgcggggtc tccattcagc ctttctcccg
      901 ctggagtcgt gaaccataat tccggtccac aatcggaaca ggcgatggtc tgggcatgaa
      961 acctccgatc tgccgggttg gtatactcat ccctgcaggc ggggcacaac tcaaaccgct
     1021 ccattgaggt tcgcacccgg tcaaagggga gcgcctccat gatggtaaaa cgaggcccgc
     1081 agatggtgca actgttgaag gcgtacttat aacgccggtc atgagggttc tggaactcgg
     1141 cgatgcaggc agggcaaatt ccctgatcgg gcaagggcga gccctccagc tgccctgctt
     1201 cactcgataa aatcgtgaaa ccggattcgg tcaggacagg cactgtttca agatcgatcc
     1261 ggtcgatcac cgccgctgac ggagccctcg agctaagcgc ctcccggaaa gcggttaacg
     1321 cctccggggt gccctgaatc tcaagctcga ccccggcgaa gttattccgg acccaaccgg
     1381 taagcgacag ttcccgggcg agattgtata aaaaaggacg aaacccgacc ccctgtacta
     1441 aaccgcttac catcaaacgc agccgtgtca gttcattttc aaccatcgtt ccaacccttc
     1501 ttgattacct cgggaatctg atgatttttc cttcatgtgt atctatcgat gcgcttcaaa
     1561 ctttatgaaa acccttcaat cccgtggcac gaggtttgaa ccggtccccc gttccccaaa
     1621 ctcttgtcgt aggccgggac tgttcaaccg gcggatcaaa atcaaaaaaa tcatgcaaag
     1681 caatccagcg cttgagccga tgaatgcctt gaaggtgtcg gccgaggctc ccaaggtgtc
     1741 cggtatggcc tgagttataa aacatattcc ggccataata ttgaaggcta aaaaaaacca
     1801 tgaaaaaaac cgaagcccgc ccagtgccca atcttcccgg cggagaaacc cgcggcaagt
     1861 gagatagagc agtaatgaga tgagtatttg caatactgca agtaaagtga agttttgcaa
     1921 tatgagtttc aaaaaccagg aatcgccata catctcagca gtcggaaagc cttgaaccaa
     1981 cattctcaat agctgatcgg tcaatattcc ccccaggccc attaaaaaaa gaaacccggc
     2041 aatgatctta aaaataacgg caatggaatt gattgttttg gaagtcgtcc gcatgaagtt
     2101 tattcaccac cgttcgaatg atgcatcgcc tgaaaaatcg caggcacctg aaaagtcgcc
     2161 gcgtagccgg aaagtcatcc cctccgaatt gcacgggaag ggggacaacc agcgaacgcc
     2221 gaatgagatg attgctttgg gtatgagatt cgctgttagc agggatttcc ctgcaacaat
     2281 aagttatcat cccgcctacc cttggtttaa gtcgggtcac cggagtccag gtattcaaaa
     2341 cgcggaacat cccggccctc tacccgtacc gtccccataa aaagcgcttc cggccgtatc
     2401 cacaattgac cttccccata aagtgccttg tagactacat acggttccag tgtttcgctg
     2461 tgattggcgg ttcccagaac ctgatattct cttcccttga aatggcggta tcgccccggt
     2521 tgaaccattc tgcctccctc aagtcggaaa agtttgcccg ttgtttatag atctgaccta
     2581 tcaa
//
LOCUS       DASVMB010000538         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_506786,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000538 DASVMB010000000
VERSION     DASVMB010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506786"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VF531_15135"
     CDS             complement(<1..1259)
                     /locus_tag="VF531_15135"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7715349.1"
                     /translation="MDSVDFSKLVKKSEALSCVMLTGSNALNQSAPDDAELAREIIAL
                     ANNGDPGYILIGVSKDGSEFFDVANPALTDQGLQEFCERYIDPVPQVRLERCKWEDAA
                     DEDLRGITMVVITVGPQWQRCFRFNRDIIDFRVECCHRKNEVWIRRDTENGSAVTVPA
                     TPEEIKNLLEAKVKPAPRVPRGTYKDYTHLPFDILLADILDELDEAVTKIGGKLVSDK
                     DPFSRENGSLWHHLVLPVNGYPMVLRLLVIEKCVTKNDYEIFCRRFLTLQHGVLLIAL
                     GEIKPDVLPGVPVKVEESWGCFFTGPYLHLGLRMYNLEMPVPRDLEEKLGDPDSMGFA
                     LAKITGNRDLRKKLEALLATLSERQDYQQLVRANQIRINAILSWYLKESCPRKADKSF
                     IPKQLLANEIWEPKRYGRVLLIRQPELT"
     gene            complement(1414..>2580)
                     /locus_tag="VF531_15140"
     CDS             complement(1414..>2580)
                     /locus_tag="VF531_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX7715350.1"
                     /translation="LVGVVLESRKFSQITKVYGAVHGVRGIIEEDFLDLTQETTHNLE
                     QVAITPSSALMSTRDKPDEKYCKEIFEVFKAHDVRYFFYIGGNDSADTVRIVNEQAES
                     SDYEFRAIHIPKTIDNDLVINDHTPGYGSAARFVAQATMGVNLDNRALPGVYIGVVMG
                     RNAGFLTAASCIAQKYPDDGPHLIYLPERAFSMDKFTRDVKQVYDRYGRCVVMVSEGI
                     SDEQGNPIITHLVDNVEKDAHGNVQLSGTGALGDLLAHYIKEKLNIKRVRSDTFGYLE
                     RSFIGCVSDVDQQEAREVGEKSAQFAIWHNLDGSITIHRTGYYSVDYKLSPLAEVAAK
                     TKKMPDEFINAEGNHITDAFKFYVRPLLGSGLPTFDRLRAPEAPKILYKDKNKK"
BASE COUNT          637 a          681 c          613 g          649 t
ORIGIN      
        1 gtcagttccg gttgccggat aagaagaacc ctcccgtatc gcttgggttc ccaaatttca
       61 ttcgccaata actgcttggg aatgaagctc ttatcagcct tgcgtggaca gctttccttg
      121 aggtaccaag agagaattgc attgatccgg atctgattgg cccgaaccaa ttgttgatag
      181 tcctgacgtt ccgataaggt tgccaagagt gcttccagtt tcttacgtaa atcccggttg
      241 ccggtgatct ttgccagtgc gaaacccatt gaatccggat cacccaactt ttcctccaaa
      301 tcacgcggta ccggcatttc cagattgtac attcttaggc cgagatgcag gtaaggcccg
      361 gtaaaaaaac agccccagga ctcttcaacc ttgaccggta caccgggtaa aacatcgggt
      421 ttgatctctc ccaaggcaat gagcagtacc ccatgctgta aagtaagaaa acgcctgcag
      481 aaaatttcat aatcattctt ggtgacacat ttttcgatca ccaacagccg aagtaccatc
      541 ggatacccat ttacgggaag taccaaatgg tgccagagag atccattttc cctactaaac
      601 gggtccttat cactcacaag tttgccgccg atctttgtca cagcctcatc taattcatca
      661 agaatgtcgg cgagcagtat atcaaagggt agatgggtat agtccttgta ggtccctctc
      721 ggaacccgag gcgcgggctt aactttcgct tcgagtaaat tcttgatctc ttcgggcgtg
      781 gcgggaactg ttactgctga accgttctcc gtatccctgc gaatccagac ttcgtttttt
      841 cgatgacaac attccacacg aaaatctatg atatcccggt tgaatctgaa gcaccgctgc
      901 cattggggac caacggtgat aaccaccatg gtgatccccc gcagatcttc atcagcggca
      961 tcttcccact tacatcgttc caaccggacc tgcgggacgg gatcgatgta acgttcgcaa
     1021 aactcctgaa gcccctgatc ggtaagcgcc ggattggcaa catcgaaaaa ttcggatcca
     1081 tccttggaaa ctccgatcag gatatatccg ggatcaccat tgttggccag cgcgatgatc
     1141 tcccgcgcca gttccgcgtc gtccggagcg gactgattga gggcattgct cccggtaagc
     1201 atcacacatg ataaagcttc gctttttttt accaatttgc taaaatctac gctgtccaaa
     1261 gaaggcacct ccgggcatga aaaatcaaat catggatgag cggaaacgga gatatctaaa
     1321 gattcctctt tgcttggaat tttcctctat tccattcaaa cgatcaatcc ataaaaaaaa
     1381 ccgggccgaa tcaggcccgg ttcttttaac ccgttacttc ttatttttat ctttgtacaa
     1441 aattttgggt gcctcgggcg cacggagccg gtcgaaggtg ggcaaacccg acccgagtag
     1501 cgggcggacg taaaacttga aggcgtcggt tatatgattg ccctccgcat tgatgaactc
     1561 atccggcatc tttttcgtct tggcagccac ttccgccaga ggcgaaagct tataatccac
     1621 cgaataatag ccggtgcggt gaatggtgat tgatccgtca aggttatgcc agatagcgaa
     1681 ctgggcagac ttttctccca cttcacgggc ttcttgctga tcgacatccg atacgcaacc
     1741 gatgaaggaa cgttccaggt aaccgaatgt atcggagcgg acccgcttga tgttgagttt
     1801 ttctttgatg taatgagcca gtagatcgcc taaggctccg gtgcccgaga gctgtacgtt
     1861 gccatgggca tccttctcca cattatccac caggtgggtg atgatcggat taccctgttc
     1921 gtcggagata ccttcagaaa ccataactac gcaacggcca taccgatcat agacctgttt
     1981 cacatccctg gtgaacttgt ccatgctaaa cgcccgttcc ggaagataga tcagatgcgg
     2041 cccgtcatcc gggtatttct gggcgatgca agaagcagca gtcaggaagc ctgcgtttct
     2101 gcccatcact acgccgatat agacaccggg caaggcacgg ttatccagat tgacgcccat
     2161 tgtcgcctga gcgacaaatc gggccgccga accgtacccg ggggtatggt cgttgatgac
     2221 cagatcatta tcgatggtct tggggatgtg gatcgcccga aactcgtaat cggacgattc
     2281 ggcttgctcg ttgacgattc ggaccgtgtc ggccgaatcg tttccgccga tataaaagaa
     2341 gtatcgtaca tcatgggctt taaaaacctc aaagatttcc ttgcaatact tttcatcagg
     2401 tttatcccgg gtggacatca atgccgacga aggggtaatg gcaacttgct ccagattatg
     2461 ggtggtttcc tgagtcaggt ccaggaaatc ctcttcaatg attccccgaa ctccgtggac
     2521 agcaccgtaa accttggtga tttgggaaaa cttccgggat tccagtacca ctcccaccaa
//
LOCUS       DASVMB010000539         8728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_65797,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000539 DASVMB010000000
VERSION     DASVMB010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65797"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="argJ"
                     /locus_tag="VF531_15145"
     CDS             <1..888
                     /gene="argJ"
                     /locus_tag="VF531_15145"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7715351.1"
                     /translation="AEQVLVASTGVIGVELPVTKIQAALKDKGFIRAEGGGAAAQAIM
                     TTDTFPKEVAVEFALGGKQVRIGGMAKGSGMIHPNMATLLGFITTDIAIDRMMLARAI
                     RSAGDYSFNRITVDGDTSTNDSLFIMANGLAGNPEINTDGPDYQLFLEGLTEVCLNLA
                     KMIARDGEGATKLVEVRTEGASSEAAALRIGKSVATSNLVKTALFGEDANWGRILAAA
                     GYSGAQIDLEKIRIYLGGLLVCQNGIGVAFDEAEAKRILEQKEILIRINLGMGDAVAS
                     VWTCDLSYDYVKINGSYRT"
     gene            1132..2016
                     /gene="argB"
                     /locus_tag="VF531_15150"
     CDS             1132..2016
                     /gene="argB"
                     /locus_tag="VF531_15150"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7715352.1"
                     /translation="MESLIAKAEILVEAIPYIRTYYGKTFVIKYGGNAMINEELKLGV
                     FQDIVLLKFLGVNPVVLHGGGPEINQMLKRIGKESQFVQGLRVTDAETMEIVQMVLAG
                     KLNKAIVAQLNRLGGKAVGLSGQDAKLLEAQKTTPTMSAGFSGPIPDIGYVGEVVRIN
                     TNILNQLIESGYIPVISSIGVGPDGESYNINADTAAGELAAALKAEKLVMLTDVEGIF
                     EDYNRKDSLISALSINRAHEMIDGGQIEGGMIPKVEACITALRGGVNRTHIIDGRQLH
                     SMLLEILTDRGIGTMVVE"
     gene            2028..3215
                     /locus_tag="VF531_15155"
     CDS             2028..3215
                     /locus_tag="VF531_15155"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HEX7715353.1"
                     /translation="MTNEEWVAVAREAIMNNVGRFPIVINRGKGSRVWDADDKEYIDF
                     LTGISVDNFGHSEPRIAAAIAAQAERIVHVSNYFYLEDQIKLARELLRLSGFHRVYFG
                     NSGAEANEAAIKLARKYGRETLGGRNGIVTAKNSFHGRTYGALSATGKPKYQENFLPI
                     VPGIVHADFNSLEDWKAKIDDNTCAILIELVQGEGGVNPADPGFIKGLREICQKRGIL
                     FMVDEVQTGLGRTGKMFAYEHYGFRPDVITIAKSLGGGVPCGAMLVNEDADVFKPGDH
                     STTIGGGGLAFAAGLVTLELLQTPGLMRQVIEKGQYIQAVWEKWRQELPVIQGYRGLG
                     LMLALELKVPSKPVALRCLEEGLIVNAVTELAIRILPALNIEQEDLDAGLEILKKVLT
                     EFV"
     gene            3352..4569
                     /locus_tag="VF531_15160"
     CDS             3352..4569
                     /locus_tag="VF531_15160"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7715354.1"
                     /translation="MDSKVKKVVLAYSGGLDTSIIIPWLKEHYGCEVIAYAADVGQGE
                     ELDPLKEKAIKTGASKIYIEDLKEELVRDFIFPMLKSGAVYEGKYLMGTSVARPVIAK
                     RQVEIALQEGADAVAHGATGKGNDQVRFELTFKALKPDLKIIAPWREWDIKSRDEEID
                     YAAKRGIPVPVTKEKPYSMDRNLWHISYEGGILEDPWNEYNNDMFIYTVAPEQAPDKP
                     EIITIDFVKGEPVAINGQGYDPVALIFKLNEIGGKHGVGRVDIVENRLVGMKSRGVYE
                     TPGGTILYEAHHALETITLDRDTLHFKGQLAGRYAELVYFGQWFTPLKSAMDAFIDKT
                     QERVTGSVKVKLYKGSVQTVGRKSPYSLYQEKLATFGADQVYDQRDAAGFINLFGLPL
                     KVQAMLDQEIKKK"
     gene            4806..5825
                     /locus_tag="VF531_15165"
     CDS             4806..5825
                     /locus_tag="VF531_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7715355.1"
                     /translation="MTIYDVAKKAGVSITTVSRVINNHPYVREETRQRVKALLKEMHF
                     IPNSNASSLVRKTTNTIAVIIPDVTNHFFTTLLRGIEDKSSENGLAVIFGNTDEEIQK
                     EQTYIQMFMERRVDGLIIAPVSPKAKNLKSIIKREIPMVLVDREVPGLAVDVVVSDNQ
                     RSAEKLVEHLIGLGHSQIAFISGPKENSVNQQRFEGYRAALQKFGIPLDERMILHGDK
                     PNKETGIRLTRVLLEQSLHPTAIFAANNFLAIGATTALQKANLAVPGDMGIVCFDDND
                     SDSVLNPFFTSVNQPAYLMGQMAMDLLQRQMKQKNIAPAKMILECELHVRNSVGIGPN
                     KVLCN"
     gene            5887..7707
                     /locus_tag="VF531_15170"
     CDS             5887..7707
                     /locus_tag="VF531_15170"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEX7715356.1"
                     /translation="MSNLHDLKTNIHQHRLQGSLPKVGIRPIIDGRRRGVRESLEEQT
                     MKMAKSAAALISENLKYPNGLPVECVIADTCIGGVAEAAQTAEKFARAGVGVSLSVTP
                     CWCYGAETMDMDPFIPKAVWGFNGTERPGAVYLAAVLAGYSQKGLPAFGIYGHDVQDA
                     GDPSIPDDVKEKILQFVRAGLAVAEMRGKSYLAMGGVSMGIAGSIGDPDFFENSLGMR
                     YESIDMSEFTRRMQERIYDPREFESGLEWVKENCKEGPDPNPTAQQVPRAQKDQDWET
                     VVKMTLIARDLMIGNPRLAELGFGEEAMGHNAILSGFQGQRQWTDHFPNGDFLETILN
                     SSFDWNGIREPFVVATENDSLNGVAMLFGHLLTNTAQIFSDVRTYWSPEAVRRVTGIG
                     LTGPVAGGFIHLINSGSTCLDGTGQQTSDGKPVMKPFWEITPEEAGRCLDATSFRYAN
                     LGYFRGGGFSTEFTTRGGMPVTMSRVNIIKGLGPVVQITEGYTAQLPAGVHDVLVKRT
                     DPTWPTTWFVPNLTGQGPFKDVYSVMNHWGANHGAVCYGHIGGDLITLAAMLRIPVAM
                     HNVPEERIFRPSVWGAFGTKDPEGSDYRACNNFGPLYGSK"
     gene            7823..>8728
                     /locus_tag="VF531_15175"
     CDS             7823..>8728
                     /locus_tag="VF531_15175"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7715357.1"
                     /translation="MDKALVMMEGIEKTFPGVHALSQCRFELRAGEVHALVGENGAGK
                     STLMKVLTGVYRKDAGRICYKGEEAEISNPKAAQELGISIIYQEFNLMPHLTVAQNIF
                     IGREPRQGIGFFLNEKVMNQRTDELLAMIHLHIDPRTKVSNLTVAKQQMVEIAKALSY
                     HSEVLIMDEPTAALTETEIADLFRIIRQLREKGVGIIYISHRLEELKLISDRVTVMRD
                     GRYIDTVLTRETEIDKIINMMVGREIYATFHSDLTGASREIVLEVKNLNRGCAIKNVS
                     FNLKKGEILGFAGLMGAGRTEVARAI"
BASE COUNT         2180 a         1976 c         2476 g         2096 t
ORIGIN      
        1 gcggaacagg ttttagtggc ctccaccgga gtgatcggcg tcgagttacc ggtgactaag
       61 attcaagctg ccctgaaaga caaggggttc attcgggccg agggaggcgg ggccgcagcc
      121 caggccatta tgaccaccga tacctttccc aaagaagttg cggtcgagtt tgctctcggc
      181 ggcaaacagg tccggatcgg cggaatggcc aaggggtcgg ggatgattca tcccaacatg
      241 gccacgttgc tcggattcat cactaccgac attgccatcg acaggatgat gcttgctcgg
      301 gcaatacgaa gcgccggaga ttattcattc aatcggatca cggtggacgg ggataccagc
      361 accaatgatt cattatttat catggccaac ggacttgccg gtaacccgga gataaacact
      421 gatggaccgg attatcaatt atttctggaa ggtttaaccg aggtctgcct gaaccttgcc
      481 aagatgattg ccagagatgg tgaaggcgca accaaactag tggaagtacg gaccgaagga
      541 gcttcttccg aagcggcggc actccggatc ggcaaatccg tggccacctc caatctggtg
      601 aagaccgcac tctttggcga agatgcaaat tggggccgga tcctggctgc ggcgggttat
      661 tccggagcgc agattgatct ggaaaagatc cggatttacc tgggtgggct tctggtttgt
      721 caaaatggaa tcggtgtggc cttcgatgag gccgaggcca aacggatcct ggagcaaaag
      781 gagatcttaa tccggattaa tttgggtatg ggtgacgccg ttgcaagcgt ctggacctgc
      841 gacttgagtt atgattatgt gaagatcaat gggagctacc ggacgtaagc gcgcccgagg
      901 ggtgtgcggc aacaggcgtg aggcatgagg cgtcgcgtcc ctgcgggacg cggggcaact
      961 caccccgcgt cacctgttac tttcagtatg actctacgcc gtgttttaac gcgacctctc
     1021 ttttcagaaa aagaggggtc gggggtgagt tgcttttaga ctatagactt cagactttaa
     1081 gactttagtc tttaggactt tcgactttat ataaatggga gtgggctgcg ggtggagagt
     1141 ttaatagcca aagcggagat tctggttgag gcgattcctt atatccggac ttattacggg
     1201 aaaacctttg tcatcaaata cggcggtaat gccatgatca atgaggagtt gaaacttggg
     1261 gtattccagg atatcgtgct tctcaaattt ctgggggtca atccggtggt gctccacgga
     1321 ggcggcccgg agattaacca gatgctgaaa cgaatcggaa aggaatccca gttcgtccag
     1381 ggactccggg ttaccgacgc cgagacgatg gagatcgttc agatggtatt ggcgggtaag
     1441 ttgaacaagg ccatcgtcgc ccagcttaac cggttgggag gcaaggcggt gggtttatcg
     1501 ggtcaggatg ccaaactgtt ggaggcccaa aagacgacgc ctactatgtc ggccggcttt
     1561 agcggcccta tccccgatat tggctatgtg ggtgaagtgg tccggatcaa tacgaacatt
     1621 ttgaatcagc taatcgaatc cggatacata ccggtgatat catccatcgg ggtgggcccg
     1681 gatggcgaaa gctataacat caacgcggat actgcggcgg gagagcttgc cgctgccctc
     1741 aaggccgaga aattggttat gctcaccgat gtggagggga tcttcgagga ttataacagg
     1801 aaagacagtc tgatttcagc ccttagtatc aaccgggcgc atgaaatgat cgacggcggt
     1861 cagatcgagg gcggaatgat tcccaaggtt gaggcgtgta tcaccgcgct ccggggcgga
     1921 gtcaaccgga cccacatcat tgatggccgt caattgcatt cgatgttact tgagatcctg
     1981 accgatcgcg gtatcggtac catggtggta gagtaggggg gttggcaatg actaacgagg
     2041 aatgggttgc ggtcgcccga gaggcgatta tgaataacgt agggcgtttt ccgatcgtga
     2101 tcaatcgggg caagggttcc cgggtttggg acgcggatga caaggagtac atcgactttc
     2161 tgacagggat atccgtggat aacttcggcc atagtgagcc gcgaattgct gccgctatag
     2221 ccgctcaggc ggaacggatc gtccatgttt ccaactattt ttatctggag gaccagatta
     2281 agttggcccg ggagctgctg cggctcagcg gttttcaccg ggtttacttc ggaaacagcg
     2341 gcgccgaggc caacgaggca gccattaaac tagcccggaa atacggtagg gaaactttgg
     2401 gcgggagaaa cgggatcgtg acggccaaga attcatttca cggccggact tacggggccc
     2461 tctccgctac cggtaaacca aagtatcagg agaattttct gcccatcgtt cccggcatcg
     2521 tccatgccga ctttaacagc ttggaggact ggaaagcgaa gattgacgat aatacttgcg
     2581 ccatcctgat cgaactggtt cagggagagg gcggagtcaa tccggcggat cccggtttta
     2641 tcaagggctt acgcgagatt tgtcaaaagc gcgggatcct tttcatggtg gatgaagtcc
     2701 agacaggatt gggacggacc gggaagatgt tcgcttacga acattacggg ttcagaccgg
     2761 atgtgatcac tatcgccaag agtctggggg gcggggtgcc ctgcggcgcc atgctagtca
     2821 atgaagacgc ggatgtcttt aaacccggcg atcacagtac gaccattggt ggaggcggtc
     2881 tggcttttgc tgccggtctg gtcactttgg aacttttgca gacgcccggg ctcatgagac
     2941 aggttattga aaaaggccaa tatattcagg cggtttggga gaagtggcgg caagagttgc
     3001 ccgttattca agggtaccgt ggtttgggat tgatgctggc cttggagttg aaggttccca
     3061 gtaagccggt ggctctacga tgccttgaag aagggttgat cgtaaatgcc gtcaccgagc
     3121 ttgcgatccg gattttgccc gctttgaata ttgaacagga agatctagac gcagggcttg
     3181 agatcctcaa aaaggtgctg actgaattcg tttagatcct taccccgcca aaagtttgaa
     3241 ttgtgaagtg tgaagtttcg aaaaagtttc cggttttgtt tcacctttca ctctacactt
     3301 agctttttac ccttttttaa ataaactatg gataaagcgg gaggttaatt gatggattca
     3361 aaggtcaaaa aggtagtgct ggcgtattcg gggggactgg atacctcgat catcattccc
     3421 tggcttaagg aacattatgg ttgcgaagtt atcgcctacg ccgccgatgt cggccagggg
     3481 gaggaactgg atcccctcaa ggagaaggct atcaagaccg gggccagcaa gatctacatc
     3541 gaggatctca aggaagaact ggtccgtgat ttcatctttc cgatgcttaa gtcgggggcg
     3601 gtttacgaag gaaagtatct gatgggcacc tctgtggccc gcccggtcat cgccaagcgg
     3661 caagtggaga tcgctctcca ggaaggggcc gatgcggttg cccacggcgc aaccggtaag
     3721 ggcaatgacc aagtccggtt tgagttgacc tttaaggccc tgaaaccgga tctcaagatt
     3781 attgctccct ggcgggaatg ggatatcaaa tcccgagacg aggagatcga ctatgccgca
     3841 aaacggggga tcccggtgcc tgtgaccaag gagaaacctt attcaatgga ccggaacctc
     3901 tggcatatca gttatgaggg cggaatcctc gaagatccct ggaacgaata taacaacgat
     3961 atgttcatct atactgtcgc cccggaacag gctccggaca aaccggagat catcaccatc
     4021 gatttcgtta agggtgagcc ggtggcgatc aacggccaag ggtatgatcc ggtggcgctc
     4081 atcttcaaac tgaacgagat tggcgggaag cacggcgtgg gccgggtgga tatcgtcgag
     4141 aaccggctgg tggggatgaa gtcccgtggt gtttatgaga ctcccggcgg gactattctg
     4201 tatgaggccc atcatgctct ggagacaatc accttggatc gtgatacgct ccatttcaag
     4261 gggcagttgg ccggtagata tgcggaactg gtctacttcg ggcaatggtt cactccactc
     4321 aagtcggcca tggatgcctt catcgataag actcaggaac gggtcaccgg ttcggtcaag
     4381 gtcaagctat acaagggcag cgtgcagacg gtcggccgga agtcgccgta ctcgctctac
     4441 caggagaagc tggccacctt cggtgcggac caggtttacg accaaaggga cgccgccgga
     4501 ttcatcaatc ttttcggcct gccgctcaag gttcaggcca tgctggatca ggagattaag
     4561 aagaaataat aataagccta ttattatcaa gccgggcgta aatgcccggc tttttctatc
     4621 gagatggata cccttcttga ttgggcacca aaaattcatc attttcagaa aaggggaaca
     4681 attgtcccgc gtcgaatgaa tatatgaaag gggtttcatg cttttttctt tggaatatcc
     4741 tgtgtttgcc ggaatattat ttattgatat tctgaaatac ttttcaaaaa tgaggcagaa
     4801 tgaccatgac catctatgat gttgccaaaa aagccggagt ctcaattaca accgtttcaa
     4861 gagtcatcaa caaccatccc tatgttcggg aggaaacacg gcaacgagtg aaagccctgc
     4921 taaaagagat gcattttatt cccaatagta acgccagcag cttggtacga aaaaccacca
     4981 acacgattgc agtcattatt ccggatgtca ccaatcattt ttttaccact ttgctgcggg
     5041 gaattgagga taaatccagt gaaaatggtc ttgccgttat ttttggcaat accgatgagg
     5101 agattcaaaa agaacagact tatatccaaa tgtttatgga gcgtcgggtt gacgggttaa
     5161 ttattgcgcc ggtatcccca aaagccaaaa atcttaagtc gattatcaaa agagagattc
     5221 ccatggtttt agtcgaccgg gaggttccag ggctggcagt cgatgttgtt gtttccgata
     5281 accaacgcag cgctgaaaag ctagtcgagc atctgattgg cttaggccac agtcaaatcg
     5341 catttatctc gggcccaaaa gaaaactctg tcaaccagca aaggtttgag ggttaccggg
     5401 cggccttgca gaaattcggg atcccgctgg atgaaagaat gattctacat ggggataagc
     5461 cgaataaaga aaccggtatt cggctcaccc gggttttatt ggaacaatca ttacatccca
     5521 ccgccatatt tgcagccaat aactttttgg caatcggcgc aaccactgcc ttacagaaag
     5581 ccaatttagc ggttcccggc gatatgggga ttgtttgttt tgatgataat gactcggatt
     5641 ccgtattaaa tccctttttt acttccgtaa atcaacccgc ctatttgatg gggcaaatgg
     5701 caatggattt attgcagcgc cagatgaagc agaaaaatat cgctccggcc aagatgattt
     5761 tagagtgcga attgcatgtt cgtaattcgg ttggtatagg tccgaataaa gtcctgtgca
     5821 actaaatatc gacgattttt agctttatgc aatttatcaa acaattaatg agggtgggga
     5881 gagagaatga gcaatttgca tgacttgaaa accaatatac atcagcacag gttacagggg
     5941 agcttgccga aggtcggtat ccggccgatc atcgacggac ggcgccgggg agtcagggaa
     6001 tccttggaag agcagaccat gaagatggcg aaatccgctg ccgcgttgat ttctgaaaat
     6061 ctaaaatatc caaacggtct tccggtggaa tgtgttatcg cagatacctg tatcggtggc
     6121 gttgccgaag ccgctcaaac tgcggaaaaa tttgcccgcg caggtgtagg cgtctctcta
     6181 tcggtgactc cgtgttggtg ttatggcgct gaaacaatgg atatggatcc ttttatcccc
     6241 aaggcggttt ggggttttaa cggtaccgaa cgcccgggtg ccgtgtatct tgcggccgta
     6301 ctggcaggtt atagtcaaaa aggattaccg gctttcggaa tatacggtca cgatgttcaa
     6361 gacgccggtg atccctcgat tccggacgat gtgaaagaaa agattttgca attcgtacgg
     6421 gccggtttag ctgttgcgga aatgcgcggc aaatcttatc tggccatggg cggggtttcg
     6481 atgggtattg ccggctccat cggggaccct gacttttttg aaaactctct gggtatgcgt
     6541 tatgagtcaa tcgacatgtc cgaattcacc cgcagaatgc aagagcggat ttatgacccc
     6601 cgggaatttg aatccggctt ggaatgggtg aaagaaaact gcaaggaggg tccggatccg
     6661 aaccctaccg ctcaacaagt acccagggcc caaaaagatc aggactggga aacggttgtt
     6721 aagatgacct tgatcgccag ggatttgatg attggcaatc cccgtctggc cgagcttggt
     6781 ttcggcgagg aagccatggg gcataacgcg attttgtctg gatttcaagg gcagaggcaa
     6841 tggaccgatc attttcccaa tggcgatttt ttggaaacca tcctaaattc ctcgtttgat
     6901 tggaacggga ttcgggaacc ttttgtggtg gctaccgaga acgatagtct caatggggtg
     6961 gcgatgctct ttgggcattt gttgaccaat accgcccaaa tattttcgga tgtcagaacc
     7021 tactggagcc cggaagcggt gcgacgtgta accggtattg gtttaaccgg tccggtcgcc
     7081 ggtggattca ttcacttaat caattccggt tccacatgcc tggacggcac cggccaacag
     7141 acgagcgacg gtaagccggt tatgaaacct ttctgggaga ttactccgga ggaagccggc
     7201 cggtgcctgg atgcaaccag cttccgctat gccaatctcg gttatttccg gggcggcggt
     7261 ttctcgaccg agtttaccac cagaggcgga atgccggtta ccatgtcgcg ggtaaatatc
     7321 ataaaaggtt tagggccggt ggttcagatc acagaaggct acaccgccca attaccggcc
     7381 ggggttcatg acgtactggt aaagcggacc gatccgacct ggcctactac ttggtttgtt
     7441 cccaatctga ccggccaggg tccatttaag gatgtatatt cggtgatgaa tcattggggc
     7501 gcgaatcacg gggccgtatg ttacggccat atcgggggcg atttgattac gctcgctgcg
     7561 atgttgagga ttcccgtcgc catgcataat gttccggaag aaagaatatt ccggccaagt
     7621 gtctgggggg ctttcggaac aaaggatccg gaggggtccg attacagggc ttgcaacaat
     7681 ttcggccctc tgtatgggag taaataaccc tcaccactta gtgaaatccc atatttgact
     7741 gttttgcttc ttaacaatgg aagcgtttag acaagatccc gaatcacctg ctgattcaat
     7801 cttaggaatt tgaggtgaca atatggataa ggctctggtt atgatggagg gaattgaaaa
     7861 aacctttccg ggtgtccatg cgctcagcca atgccggttc gaactacgcg ccggtgaagt
     7921 acacgctctg gttggagaaa atggggccgg taaatcgaca ttgatgaaag tgctcaccgg
     7981 ggtgtatcgg aaagatgccg gccgcatctg ttacaaaggc gaggaagcgg agatttcaaa
     8041 tcctaaagcg gctcaggaat taggtatcag cattatttat caagaattca atttgatgcc
     8101 ccatctgacc gtggctcaga acatttttat cggccgcgaa ccgcgccaag gcatcggatt
     8161 tttccttaat gaaaaagtga tgaaccaaag aaccgacgag ttactggcga tgattcatct
     8221 tcatatcgat ccgcgcacta aagtatcaaa tcttaccgtt gccaaacaac agatggtgga
     8281 gatcgccaaa gctctatcct atcattccga agtgttgatt atggatgagc ccacggcggc
     8341 gctgaccgaa accgagatcg cggatctctt ccgcattatc cgtcaactcc gggaaaaggg
     8401 agtgggaatc atctacatat cccatcgact cgaagagtta aaactgatct cggaccgggt
     8461 aacggtaatg cgtgatggac gttatatcga cacggttctt acccgtgaga cggagattga
     8521 taagattatc aacatgatgg tgggccgaga gatctatgcg acttttcatt ccgatttaac
     8581 cggtgccagc cgggagattg tacttgaagt caaaaattta aaccggggct gtgccattaa
     8641 aaatgtgagt tttaacctga agaagggaga aatccttgga tttgcgggac tgatgggtgc
     8701 cggtcgcacc gaggtggcgc gggcgatt
//
LOCUS       DASVMB010000540         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_580824,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000540 DASVMB010000000
VERSION     DASVMB010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580824"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VF531_15180"
     CDS             complement(<1..701)
                     /locus_tag="VF531_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7715358.1"
                     /translation="MGVTIRDVAKAAEVSVATVSRVLNNSPSVSPNTCERVKKAMKML
                     DFFPNSIAKNFAQQSTFTVALIIDIDKPTSFLNPFFHEVLDGIEKVVYSQGYYLLIVN
                     ANTMVGNETALSRLILEKRVDGLILPSSILEKNLIGMMKKDEFPVVLIGEPEMKVDID
                     WVDINNVLAGEQAVRHLIENSYRKIAFMGGNPNDLFYKNRLAGYQSILEKSGIQYDPA
                     IIKPGIDSKENGARL"
     gene            complement(881..1321)
                     /locus_tag="VF531_15185"
     CDS             complement(881..1321)
                     /locus_tag="VF531_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715359.1"
                     /translation="MKALKPWWILVMMLGAVIILVMGLVGATQDSFTTNVVPVWSIFS
                     MDSGSWKTILYHPKGMPVDEPGKLPQIPKEFYKAHEISVSFCIDWERPTTPPWGQPVF
                     IKPLRQRSGFFQPVTAIMQFFVFYQCARNHSKEEPYDLFCKFVL"
     gene            complement(1335..1490)
                     /locus_tag="VF531_15190"
     CDS             complement(1335..1490)
                     /locus_tag="VF531_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715360.1"
                     /translation="MDWFTWGTVMVVVAIYLLFFLAARRAEKVVDAKAKMARAAMMKS
                     GAKARKA"
     gene            complement(1572..>2882)
                     /locus_tag="VF531_15195"
     CDS             complement(1572..>2882)
                     /locus_tag="VF531_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX7715361.1"
                     /translation="LPPFFILLIWEVNHSTGAFPIPLYICAPFFILILAMAVLPQLWP
                     EFWERDLNKLMIAVLCALPIVVFFGTGSSRQLVAAIDEYLAFIVLIGALFVITGGIVL
                     EGDLPGTPAANTIFLLVGAVIANFIGTTGSSMLLIRPWLKMNCQRHYTRHLPVFFIFL
                     VSNIGGALTPLGDPPLFLGYLRGVPFEWNLNLFPHWGITIALLLTTFYLWDTRCFKRE
                     PEQIRKGLRFPFRFCSIRITGAFNLWLLLAVIAVILLRVITPWREMIMIMLALISLGF
                     TPGSLRSKNRFTWHPLIEVAMLFAGLFITMRPVMMILEAKGAAFGINQPWQFFWLVGG
                     LSSFLDNAPTYATFFALATNVTRTISDGFPVIAGVRGDLLVAISCGAVFMGANSYIGN
                     GPNLMVRSIAEAQDFETPQFFEYLGYSFAILVPVFLVLTGLFFI"
BASE COUNT          873 a          709 c          602 g          698 t
ORIGIN      
        1 atcagtctgg cgccgttttc tttcgaatca atccccggtt taattattgc cggatcatat
       61 tggataccac ttttttctaa tatgctttga tacccggcca aacggttttt atagaaaaga
      121 tcattcggat ttcctcccat aaaagctatt ttacggtagg aattttcaat taaatgccgt
      181 acagcttgtt ccccggctaa aacattattg atatccaccc agtcaatgtc tactttcatt
      241 tcaggttcac caataagcac caccgggaat tcatctttct tcatcattcc aattaaattt
      301 ttctccagga ttgatgaagg caatatcagg ccatccactc ttttttcaag tatgagacgg
      361 ctcaaggccg tttcattgcc aaccatcgta tttgcattga caattagaag gtagtaccct
      421 tgagagtaca caactttttc aataccatcc aatacttcat gaaaaaatgg gtttaaaaat
      481 gaggtgggtt tatcgatatc aataatcaag gcaacagtaa aggtactttg ttgtgcaaaa
      541 tttttggcga tactgtttgg aaaaaagtcc agcatcttca tggccttttt tacacgttca
      601 catgtatttg gagaaaccga cggggaatta ttaagaaccc tcgaaacagt cgctacggaa
      661 acctcagcgg ctttcgccac atcacgaatt gttactccca tttcgtttac ctcattcaac
      721 aatttgatgt aaacagttac atatatatat aatagataag tcagcaaaat tagcatataa
      781 ttttgtaacc ggttacattt atatgtaaaa caactaaatt agttataaca caaaccacac
      841 cccaatgcaa gattctgtca cccaaatcga ataaattttt ttatagaaca aatttgcaga
      901 atagatcata cggttcctcc ttagagtgat tccgggcgca ttgataaaag acgaaaaact
      961 gcatgatcgc cgtgacgggt tgaaaaaatc ccgatcgctg tcgtaacggt ttgatgaaca
     1021 ccggttgccc ccacggaggg gtggttggcc gttcccaatc aatgcaaaat gataccgata
     1081 tctcatgagc tttataaaac tccttcggta tctgcggtaa cttccccggt tcatcgacag
     1141 gcatcccttt ggggtgatat aaaatcgtct tccagctgcc cgaatccatg gagaaaattg
     1201 accacaccgg aaccacgttg gtggtaaaag aatcttgggt agcgccaact aagcccataa
     1261 ccaatatgat tacggctccc agcatcatta ccagtatcca ccacggtttc agtgctttca
     1321 atgccaactc tcccttaagc ctttcttgct ttggcccctg acttcatcat ggcggcacgt
     1381 gccatcttgg cctttgcgtc cactaccttc tccgcccgac gggccgccag gaaaaagaga
     1441 aggtagatgg caacgactac cataactgtt ccccaagtaa accagtccac tggtatcacc
     1501 tcctttcaat agccgatacg tagcaagcat tcgtatcgga aaccacctca tagagaaact
     1561 gaattgtaac cttaaatgaa gaataacccg gttaagacca ggaaaaccgg taccaatatc
     1621 gcgaacgagt accctaaata ctcaaagaac tgtggcgttt caaaatcttg agcttcggca
     1681 atcgaacgga ccatcaaatt cggaccgtta ccgatataag agttggcgcc cataaagact
     1741 gcaccgcaac tgatggctac caataggtcc ccgcggactc cggcgattac cgggaaaccg
     1801 tccgatatcg tccgggtcac attggtcgcc agggcaaaaa aggtcgcgta ggtaggggca
     1861 ttatccaaaa aggagctcaa cccgcccacg agccagaaaa attgccaggg ttggttgata
     1921 ccgaatgccg ccccctttgc ttccaagatc atcatcaccg gccgcatggt gatgaatagc
     1981 ccggcaaaga gcattgccac ctcaatcaaa ggatgccagg tgaaacggtt cttggaacgt
     2041 aacgagccgg gtgtaaagcc caaagagatc aaagcaagca tgatcataat catctcgcgc
     2101 cacggtgtga taacccgcaa caatattaca gcaatgacag ctaataacaa ccatagatta
     2161 aatgcacctg tgatacgtat cgagcagaac cggaaaggaa atcgtaggcc ttttcggatt
     2221 tgttcgggtt cccttttaaa acatcgggta tcccatagat aaaaggtcgt caacaataag
     2281 gcgattgtta tcccccagtg gggaaatagg ttcagattcc attcaaatgg aacgccccgt
     2341 aaatacccca gaaacaacgg cggatccccg agcggtgtca aagcgccgcc gatgttggaa
     2401 accaaaaaga tgaagaaaac cgggagatgc cttgtatagt ggcgctgaca gttcatcttc
     2461 agccagggcc ggattaggag catactggag ccggtggttc caataaagtt ggcaatgacc
     2521 gccccaacca gaagaaaaat cgtattggcc gcgggtgttc ccggcaaatc cccctccagt
     2581 acaatccccc ccgtaatcac gaataaggcg ccgatcaaga cgatgaaggc aaggtattcg
     2641 tcaatcgccg ccaccaattg ccgtgatgac ccggtgccga aaaaaacaac aattggtaac
     2701 gcgcagagta cggcgatcat caatttgttc agatcccgct cccaaaactc cggccaaagt
     2761 tggggaagta cggccatcgc caagatcaga ataaaaaagg gagcacatat ataaagcggt
     2821 atcggaaagg cacctgtcga atggtttacc tcccatatca ataaaataaa aaagggtggc
     2881 aa
//
LOCUS       DASVMB010000541         8442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_656387,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000541 DASVMB010000000
VERSION     DASVMB010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656387"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF531_15200"
     CDS             <1..559
                     /locus_tag="VF531_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7715362.1"
                     /translation="YIRMFSFFIPSREDPAKYRDEVMRRWKSFIDAAAGSGLILLHEN
                     EKEIYGDTPERCLDILKTMNCNYLKAIFDPANFVQCNVETYPHAFELLKDEVIYLHIK
                     DALVSDHSVVPSGYGDGKVREILTALTKRGYYGFLSLEPHLQQFRGFAGLERNAASIS
                     KDIPTDGPGKFRIAYEALQKVIGEI"
     gene            579..1787
                     /locus_tag="VF531_15205"
     CDS             579..1787
                     /locus_tag="VF531_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7715363.1"
                     /translation="MEKVRIGIIGMGNMGTNHAKKIAAGEIPGAELTAICDPRPDRLK
                     WARENLGEKVQLFETPEAMFGADVEKGSGSAGFAAPTSKIIEAVIVATPHYDHPGMAI
                     RCFQNNLHVLVEKPAGVYTGQVREMNEAAAKSGKVFGIMYNQRTNPIYQKLRELIRQG
                     ELGEIKRTNWIITSWYRSQSYYDSGGWRATWAGEGGGVLLNQDPHQLDLWQWTCGMPK
                     RVRAFMAFGKYHTIEVEDDVTAYVEYENGATGVFVTSTGETPGTNRFEVTGDRGKIVI
                     EDEKLTFWRLVVPERQFNREYQGGFGEPEHWKCEIPVKGTATQHVGIMSNWVDAILKG
                     TPLLAPGNEGIKGLEISNAMHLSAWTDRMVELPIDEGLFYEKLQEKISQSTFKKAATG
                     NQTLDVTGTH"
     gene            complement(1891..2286)
                     /locus_tag="VF531_15210"
     CDS             complement(1891..2286)
                     /locus_tag="VF531_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7715364.1"
                     /translation="MKRIIPNLAVDNCKEALDYYKKILGGEIKNVQLADGKEMFQGHE
                     GKIVHAELHINADCILYLNDLFDEKEVTSNISLMLELNNMEEINDLYSSLSQKGNVVF
                     ELQKTFWGSHHAVVTDCFGTTWSLDYSGK"
     gene            complement(2712..3980)
                     /locus_tag="VF531_15215"
     CDS             complement(2712..3980)
                     /locus_tag="VF531_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7715365.1"
                     /translation="MKVVYPICCGVDVHKTFLVATIITSQGFTPHYSKKRFSTFNNSI
                     LQFRQWLIDNNCFDVCMESTGKYWVPVYNLLEDTIRITIANPKWVKAIKGNKDDTKDS
                     KWIGDLFRLGLVPGSYIPKKSIRILREYTRYRSKLVSCKSSEKNRFQNAFTVCNVALD
                     AVVSDMFGKSASSITDYLISSDSFDPEYCSSLLQKSLKKKAATVVESIEGYQMTKEQK
                     ERMVIVRSHLDFIQNSIATVDEKLEKMVTPYQSAITLLCTIPGVDRTSAVTIISEIGT
                     DMSQFASSKRLCCWAGLTPGNNQSAGKKKSVRITRAGVYLKPALVQVAHAAVKSNRSP
                     YYRIKFERICKRRGKKRAIIAIARMILTAIYHMFVTGEVFNPSDLYKIDMPQEMLQKQ
                     KEKAIKQAIKQAIKLLISNGLIKAEDISAA"
     gene            4289..4705
                     /locus_tag="VF531_15220"
     CDS             4289..4705
                     /locus_tag="VF531_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715366.1"
                     /translation="MSIKIETFKKLRPTETYACTALEAMAWAKGLADLRIDFGKKRTF
                     QWDARALNRPKIEGLVVASVLVNRQLKPILNFYPISASRYPAEVNKAFREEVLLDLRN
                     WVEEQLAQGADRVLETEILLVELQGKSFKTHCLKYL"
     gene            complement(4898..5335)
                     /locus_tag="VF531_15225"
     CDS             complement(4898..5335)
                     /locus_tag="VF531_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715367.1"
                     /translation="MKDKYMIGALAGTIGALIQDAYGYLVKLTGLSDRGFIDFARAVV
                     FYDVKNGTAEMIVASIAHIIWDLFLGILFVYIINSMSNSYYFLKALIYGLFLWFIIQV
                     LGTLFRLPMFFHIPARAAIITLIGAVIYSLGIALIIKVLQLHA"
     gene            complement(5607..7853)
                     /locus_tag="VF531_15230"
     CDS             complement(5607..7853)
                     /locus_tag="VF531_15230"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715368.1"
                     /translation="MVINKRIKRVFIENKTQYIGSILLIIFSCFLFTGMVLVGANLKR
                     LTNEFENRYMQEDASFTTDKSIDNLQELELASNAVIEEGKTFDYTLSEGITLRIFSKN
                     DRVNIPAIIKGKELSGSGDILINPAFAAAQKLKIGDVLTILDKPFTVAGFMALPNYIY
                     PLQSETDIMYFPQNFGIAVISREDFFALGKGSNFYAVKFNHPEEHPRAQSAKFIELLK
                     SRGIGIVQWTDIGSNSRVRSVTMKNDSMNLMSKAVPTAVLLLTTILLGNVIGRLIERE
                     SVIAGALYALGYKRKEIYRHYLKFPLVIALTGGIVGTILGIFYVRPMLLFMLGYFDIP
                     LTGIDYSPLVMIISLLLPVLFLGLSGWLVIGQELSHSPVELMKGDKEKSRVNFLEHLL
                     KLEKLKFAAKFKIREQLRSLSRLALLLAGCAAATLLLLYGFFLKSGMDYLLTNSLKGA
                     YNFQYEYWFKNLQYGRPPAGSEPFSGSKFLPVGDDNRDFYVVGTLPDSKLYTLVNESG
                     TRLRMNQVIITKPLADQLQVKQGGTVTIVRKADYQEFSLKVGSIADSYSRFIFMPLAE
                     YNKKFGMRSGSYTGLGSNELLDIPENQPYSVVSMEDKAAAVRGSMAPVVSLVVFVSVV
                     AFIIGMIVIYVVTSMIIEENKRTISLMKIFGYRKKEVNSLILNSCSVVVVIGYIIGVP
                     LCFAAMGGLLKALENSITFSMPVMIDPLYIGVGFVVVMLSYEVSKLLCRKKVNAVSMS
                     EALKSEME"
     gene            complement(7853..>8442)
                     /locus_tag="VF531_15235"
     CDS             complement(7853..>8442)
                     /locus_tag="VF531_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715369.1"
                     /translation="LGPSGSGKSTLMNIIGGIDHGDSGQVSVDGVEINNLDDNKLTDY
                     RRGDVGFVFQFYNLVPNLTVGENIEVVSNISQSPLNTDEVLAAVGMKDKKHRFPRELS
                     GGEQQRVSIARAIVKNPKLLLCDEPTGALDFQTSRSILQLLQQVNKKYGTTILMITHN
                     AAIAAMANRVFKLRSGEIVEETINQTTVPAERIEW"
BASE COUNT         2360 a         1839 c         1955 g         2288 t
ORIGIN      
        1 atatatccgg atgttcagtt ttttcatccc gtcaagggaa gatcccgcca agtatcgtga
       61 tgaagtgatg cggcgttgga agagttttat tgatgcagcg gcggggagcg gcttgatttt
      121 gctgcatgag aatgagaaag agatatatgg cgatacaccg gaacgctgcc tggacatttt
      181 gaagaccatg aattgtaatt atctaaaggc gatcttcgat ccggccaact ttgtccagtg
      241 taatgtggaa acctatcccc acgcgtttga attattaaag gatgaagtga tctatctgca
      301 tatcaaggac gccttggtat ccgaccatag cgtcgtgcct tcgggttatg gcgacggtaa
      361 ggtgagggag attttaaccg cactcaccaa acggggctat tacggatttc tctcgctgga
      421 accccatctc cagcagtttc gtggttttgc cgggctggaa cgaaatgcgg ccagtataag
      481 caaagacatt cccactgacg gaccgggcaa gtttaggatt gcttatgagg cgttacagaa
      541 ggtaatcggg gaaatttaag ggtcaaggag gcatcatcat ggaaaaagta cggattggta
      601 ttatcggaat gggtaatatg ggaaccaatc atgcaaaaaa gattgcagcc ggagaaatcc
      661 ccggagctga actcacggcg atctgcgacc caagacctga tcgcctaaag tgggctcggg
      721 agaaccttgg ggaaaaggtg caacttttcg agaccccgga agcgatgttt ggcgcggatg
      781 ttgaaaaggg gagcggatcc gccggatttg ccgcgccaac atccaagatc atcgaggcgg
      841 tgatcgttgc gacgcctcac tatgaccatc ccggaatggc aatccggtgt tttcaaaaca
      901 acctccatgt gttggtggag aaacccgccg gcgtatatac cggacaagtc cgtgagatga
      961 atgaagctgc ggcaaagagc ggcaaggttt tcgggatcat gtacaatcaa cggaccaatc
     1021 caatctacca gaagttaaga gagctgattc gccaggggga attgggcgag atcaaacgga
     1081 ctaactggat tattaccagc tggtaccgtt cccaaagtta ttatgattcc ggcggttggc
     1141 gggctacctg ggctggagaa ggcggtgggg ttcttttaaa ccaggaccct catcaactcg
     1201 atctctggca atggacctgc ggcatgccga aacgggtccg cgcctttatg gcgttcggga
     1261 agtaccatac cattgaggtt gaggatgatg ttacagccta tgtggaatac gaaaacggcg
     1321 ctaccggagt tttcgtgact tcaaccgggg agacaccggg gaccaaccgt tttgaagtga
     1381 ccggagacag gggcaaaatt gtgattgaag atgaaaaact gaccttttgg agactggtcg
     1441 ttcccgaacg gcagttcaac cgggaatatc aaggcgggtt tggtgaaccg gaacattgga
     1501 aatgtgagat cccggtcaag ggaacagcca cccagcacgt ggggatcatg agcaattggg
     1561 tggatgccat cctaaaaggc actccgttac tggcaccggg caatgaagga atcaaagggc
     1621 ttgagatttc caacgccatg cacctttccg cctggaccga taggatggtg gaacttccta
     1681 tcgatgaggg tttattctat gaaaagcttc aggaaaaaat cagccaatcg acctttaaaa
     1741 aggcggcaac cggcaatcaa accctggacg taacgggaac tcattaagga gatccttctc
     1801 ttatttccga tttgtattcc ctgcggtgaa ataagaagca cgctacctca attggtagcg
     1861 tgcttcttat aagaaacgtt attgcgtcaa tcactttccg gaataatcta acgaccaggt
     1921 cgtcccgaaa caatccgtta ccaccgcgtg atgagaaccc caaaaggttt tttggagctc
     1981 aaagactaca ttgccctttt gggagaggga tgagtaaagg tcgttgatct cttccatgtt
     2041 gtttagttca agcatcagac tgatattgct ggttacttcc ttttcatcaa agagatcatt
     2101 caggtatagt atacaatcgg cattgatgtg cagttcggcg tggacgatct tgccttcatg
     2161 gccttgaaac atttctttgc cgtctgccaa ctgaacattt ttgatttcgc cccccaagat
     2221 tttcttataa taatcaaggg cttccttaca attatcaacg gccaaattgg gtataatccg
     2281 tttcatgggc ttcgggctcc tttatttaga ctggtaattt tatcttcagg cttttttaat
     2341 ataacagagt ttagtgtgga tttgaagata aaatttattc gtgctgcata tccccgcccg
     2401 gtaagggtcc gggaaagttg ttaattatat attaccaaaa caaaggagaa ctgattatta
     2461 cggttattga tttaaaaagg atttagcaca atgaggaagg aagtggcttt tttaaagtag
     2521 aaataggtat ggaaataatg taaccattat cggtactaag gatggaaaga ataatccgga
     2581 tcgtgtttgg aaagatcatt gttaatttga tcaaagttcg taccagaggc atagcgtgaa
     2641 aaatgccgga taagcaaaaa aagggcgtac gaaactaaag cctttaagcg gtctaaaaat
     2701 caagccctga tttaggccgc agaaatatct tcggccttta taagaccatt agaaatgaga
     2761 agtttaattg cttgtttaat agcttgttta atagctttct ccttttgctt ttggagcatt
     2821 tcttgtggca tatcaatttt ataaagatca cttggattga acacttcacc ggtaacaaac
     2881 atgtggtata tagcggtgag gatcatcctg gcgatagcga taatggctct tttcttgcct
     2941 cttcgtttac aaatgcgttc aaacttgatt ctgtagtaag gtgaacggtt cgatttcacg
     3001 gctgcatgag caacttgtac caatgcaggt ttgaggtaaa caccggcacg tgtaattcga
     3061 acagatttct tcttaccagc agattggttg ttgccaggcg ttagtcccgc ccagcagcac
     3121 agacgcttgg aactggcaaa ttgagacatg tcggtgccaa tctcggagat gatagtgacg
     3181 gccgaagtcc tatcaactcc gggaatggta caaagaagag taatggcgct ttggtaggga
     3241 gttaccattt tttcaagttt ttcatccacc gtagcaattg aattttggat gaaatcaagg
     3301 tgagaacgaa ctattaccat acgttccttt tgttccttgg tcatctgata tccttcaatg
     3361 gattcaacaa cggtagctgc tttctttttc aaagattttt gaagaagtga agaacagtat
     3421 tccggatcaa aagaatcgga ggaaatcaag tagtccgtga tggaagaggc agatttgcca
     3481 aacatgtcgg agacgacagc atcaagggcc acattacaaa cagtgaaagc gttttgaaaa
     3541 cggttctttt cactgctttt acaggaaaca agtttggaac ggtagcgagt atactcgcgg
     3601 agaatgcgaa ttgatttttt cgggatataa cttcccggga ctaagccaag gcggaaaagg
     3661 tcgccaatcc atttggaatc tttagtatca tccttgtttc ccttgatagc cttgacccat
     3721 ttagggttgg cgatggtaat acggatggta tcttccaaaa gattatagac tggcacccaa
     3781 tattttccgg tggactccat gcacacatcg aagcagttgt tgtcaatcaa ccactgtctg
     3841 aattgaagaa ttgagttgtt aaaagttgaa aatctttttt tggagtaatg cggggtgaat
     3901 ccttgcgagg tgatgattgt ggcgacgaga aaagttttat ggacatcgac gccacaacaa
     3961 atagggtaaa caactttcat gccaaacctc cttcaaggta ataattttcc aaggaggaag
     4021 gcattgactg aatcatcaca catttaacgg atagctaaat caattcttag cgttcggtct
     4081 gaaagatcca cttatttgtg cttggaagat gattcttaca ctgattaaaa tactgttttg
     4141 ggctaagaag gtcgatctac gactcctcct accgtgcttt gtagtttacc ttctcctcaa
     4201 atcaaagata acatttttga cgaaagtcgc ataccttttt ttcataacta tttgtgccgc
     4261 ctgcgacagc ggcggaatgg agattaaaat gagtataaaa atcgagacct ttaaaaaact
     4321 ccgtccgacg gaaacctatg cgtgcaccgc actcgaggca atggcttggg ccaagggact
     4381 tgccgatctg cggatcgatt tcggaaaaaa gcggacattt cagtgggatg cgcgcgcgtt
     4441 gaaccgaccg aaaattgaag gcctggtggt agccagtgtt ttagttaacc gccaactgaa
     4501 gccgatcctg aatttttacc cgatctcggc ttcgagatat cctgctgaag tcaataaggc
     4561 atttcgggaa gaggttcttc ttgatctgcg aaactgggtc gaagaacaac tggcgcaagg
     4621 cgcggatagg gttttggaaa ccgagatcct ccttgttgaa ttgcagggaa aaagctttaa
     4681 aacgcattgc ttgaagtatt tataaccctg aaaccattag gcatttcttc attattggat
     4741 aatacaaaga gagggaaacc tctcttttca ttctactttt tgttgtgttt taaagtcagg
     4801 caagaaatgg ctttgcactt atagagtggg agaatgggtc gccagacttg gaggggttct
     4861 aactgcccac cggtgactca aaatgtgtta tcccatatca agcgtgaagt tgaagaactt
     4921 taataatcaa agctatccca agactataaa taacggcgcc aattaaagtt ataatagctg
     4981 ctcgggctgg aatatgaaaa aacatcggca agcggaataa agttcctaac acttgaatta
     5041 taaaccataa aaataaaccg tatatcaagg ctttaagaaa ataataacta ttagacatac
     5101 tgttaattat atatacaaac aatattccca aaaataaatc ccaaataata tgggctattg
     5161 aggccactat catttcagcc gttccatttt ttacatcata aaaaactact gccctggcaa
     5221 aatctataaa tccccgatca cttaagcccg tcaattttac taaatatccg tacgcatctt
     5281 gaatcaaagc gccaattgta ccggctaggg cgcctatcat atatttatct ttcaaaaaaa
     5341 tcacctctga tcatagtttg acttattttg ggattggtat accgtttggg agggatacca
     5401 ttgcctggct tttttatgga acacctttgg ggtgtggttt acgaagccat ggtgaatagc
     5461 agaggctgcc ccagtgtttg acttccgggg cagcctttat ttaatttctt cgtcttagtt
     5521 tttgcggcaa aacccgatac cattttgaca tattttgaag atggggaagg ataagacatg
     5581 gacaaagagc caagggatgt ccgctgttat tccatttcgg attttaaggc ttcactcatc
     5641 gaaactgcat tgactttttt cctgcacaag agtttcgaaa cttcatagga gagcattacc
     5701 acaacaaatc caactccaat gtaaagcgga tcgatcatta cgggcattga aaaagtaatg
     5761 ctgttttcaa gcgctttcaa caatcctccc atggctgcaa aacagagcgg tactccaatg
     5821 atatacccaa tcacgacgac aacgctacag ctgttaagaa tcaaggaatt gacttccttc
     5881 ttccgatatc cgaaaatctt catcagcgaa atcgtgcgct tgttttcttc gatgatcata
     5941 gaggttacaa catagatgac aatcattccg atgataaatg ccaccaccga aacgaaaacg
     6001 accagtgaaa ccacaggcgc catggatccc cttacggcag cggccttatc ttccatggac
     6061 actacgctat acggctggtt ttccggaata tccagcagtt cattgctgcc aagccccgta
     6121 tagcttcctg accgcatccc gaatttcttg ttatactccg ccagcggcat aaagataaac
     6181 ctgctatagg aatcggcgat gctgccgacc ttcagcgaaa attcttgata gtccgctttt
     6241 cggacgatgg ttacagtgcc tccctgtttt acctgcagtt gatcggcaag cggcttcgtg
     6301 ataatgacct gattcatacg gagccttgtg ccggattcat ttacaagtgt atacaatttg
     6361 gaatccggta gggtgcccac gacgtaaaaa tctctattat catcacccac cggcagaaat
     6421 ttcgatcccg agaaaggttc ggagcccgcc ggcggccgcc catattgcag gtttttgaac
     6481 caatactcgt actggaaatt gtatgcgcct tttaggctgt ttgtaaggag ataatccata
     6541 ccgcttttta ggaaaaaacc gtagagcaga agcagtgtcg ccgcggcgca gccagcgagc
     6601 agcaaggcga gccgggacag acttcggagt tgttccctga ttttgaactt tgcagcgaat
     6661 ttgagttttt caagcttgag cagatgctcc aggaagttga ccctgctctt ttctttatcc
     6721 cctttcatca gctcgacggg cgaatggctg agctcttgac ctatgacaag ccagccgctt
     6781 aatccaagga ataatacggg aaggagaagg ctgatgatca ttacgagcgg gctatagtcg
     6841 attccggtca gtggtatgtc gaagtaacca agcataaaca acagcatcgg acgtacatag
     6901 aatatgccaa gaatggttcc aacgatgcca cctgtaaggg ctattacaag cgggaatttc
     6961 aaataatgac gatagatttc ctttctttta taccctaacg catacaatgc gccggcgatc
     7021 actgattcgc gctcgattag ccgcccaata acgttgccga gcaaaatagt ggtcaacaat
     7081 aaaaccgcag taggcacagc cttactcata agattcatgg agtcattctt cattgtgaca
     7141 gatctaaccc gactattact gccgatatcc gtccattgca cgataccaat gccgcggctc
     7201 ttaagcaact ctatgaattt tgccgactga gcgcgagggt gttcttcggg atggttgaac
     7261 ttcaccgcat agaaattact tcctttaccc aaagcgaaga aatcctcgcg gcttatgact
     7321 gcaataccaa agttttgcgg aaaatacatg atatccgttt ccgattgcag cggataaata
     7381 taatttggca gcgccataaa tccggcaacc gtaaacggtt tatccaatat cgtcagcaca
     7441 tcgccgattt taagcttttg ggcggcggca aaagccgggt tgatcagtat atcgccgctg
     7501 ccgcttaatt ctttaccctt gatgatggct gggatattga ctctgtcatt cttgctaaaa
     7561 atgcgtagcg ttatcccttc tgaaagcgtg tagtcaaaag tcttaccttc ttctatcacc
     7621 gcgtttgaag ccagctcaag ctcttgtaaa ttgtcaatgc ttttgtctgt tgtaaaagag
     7681 gcatcttcct gcatgtatct gttttcaaat tcattcgtca gccgctttaa attagcgcca
     7741 accaaaacca tgcctgtaaa cagaaagcaa ctgaaaataa tcagtaaaat ggagccgata
     7801 tattgtgttt tgttctcgat gaaaacacgt ttgattctct tattaatgac cattaccatt
     7861 caatcctttc cgccggaaca gtcgtctgat ttattgtttc ttcaacgatt tccccgctcc
     7921 gcaacttaaa caccctgttg gccatagcgg caatggcagc attgtgggtt atcattaaaa
     7981 tggtggtccc gtattttttg tttacctgct gcaaaagctg cagaatgctg cgggacgtct
     8041 gaaaatccag cgcgccggtc ggctcgtcgc aaagcagcag cttcggattc ttgacgatcg
     8101 ccctggcaat ggagactcgt tgctgctcac caccactaag ctccctgggg aagcggtgtt
     8161 ttttatcctt cattcctacc gccgctaaaa cttcgtcagt attcagaggc gattggctaa
     8221 tgttggaaac aacttcaatg ttttctccga ctgtaaggtt ggggaccagg ttataaaact
     8281 ggaacacaaa accgacatcc ccgcgtctgt aatcggtcag cttattgtca tctaaattgt
     8341 ttatctcgac cccgtcaaca gaaacctgtc cgctgtcgcc gtggtcaatg ccgccaatga
     8401 tattcatcaa cgtggatttt cctgaaccgg atggtccaag ga
//
LOCUS       DASVMB010000542         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_365361,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000542 DASVMB010000000
VERSION     DASVMB010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365361"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF531_15240"
     CDS             complement(<1..630)
                     /locus_tag="VF531_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715370.1"
                     /translation="MATDTTVKKEEQVDGRPKIQCHLISNTHWDREWRYSAQRTRYML
                     SYLMEMLFDIFEKEPKFKHFHLDSQTLPVQDYLEAFPEKADQLRKAVSEGKLAIGPWF
                     CLPDEFCVGGESLIRNLLLGHKIARNFGGVSKTGYSPFGWGQISQLPQIYQGFGIDVV
                     SFYRGINTYIAPRSEFVWESPDGSRVLGSRLGARPRYNIWYIIQRPVYWN"
     gene            complement(632..1627)
                     /locus_tag="VF531_15245"
     CDS             complement(632..1627)
                     /locus_tag="VF531_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7715371.1"
                     /translation="MEILATIPGIGPVDGNSGLIAIGRSSDGIFETGKNGVTSVTATG
                     DRKVEFIAFTDHTVALVKSTMGYPAYYPVHPVELKLPLKAVLMDLDGTSVRSESFWIW
                     IIQMTTASLLGKPGFELEEADLPYVSGHSVSEHLKYCIQKYCPNRKVEDARRFYFEHT
                     HREMQAILEGRGRKNAFVPTPGLKDFLCELKSMRIKIGLVTSGLYEKAWPEILSAFQT
                     LKMGDPKDFYDAVISAGFPLRQGEAGTLGELSPKPHPWLYAETGRVGLGIEFGERNSV
                     IGIEDSGAGICSIRLAGYPAVGFAGGNIIESGTRALCNYYCRNFTEILDLLKEDN"
     gene            complement(1629..2927)
                     /locus_tag="VF531_15250"
     CDS             complement(1629..2927)
                     /locus_tag="VF531_15250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715372.1"
                     /translation="MGLLPSFRPLNQKPAPLSSGQVEHGIRITLIEGVIAALFTVLIG
                     GTFFNAFAIQLGANTQQLGLIASLAPLSTLIQLAYPGLQNKTRSRRRLLMTIIIPDRF
                     LLWGVIFIPILLPRPLWIPFLLGTILLRSLASQLNNLIYQDWLADLIPIDRRNSYWGL
                     RYTLGGPVGIVIPFAAGLVLDRCHGFSGFQILYWIALPLIVFDILLFLYQDEPPYRSH
                     RSIDGRHLLHCLHVKEFRNYMVFFFGWTVFQGMLTPYTSYIMFKVYHLPYVLVSGLTV
                     LATVTSTILYVTWGKIQDKYGVVSVYRYVLPGIALVSGLWAFVTANSYGLYILIFLLT
                     GALNAATLVGGFCLLLSVIPGEARSEFLTINSVSIGIAGFLASNLGAAMLELFERLKP
                     KAVGLDFSPLQWLFFLGSICSLLLALAFGKFVKPFPVQED"
     gene            complement(3025..>4451)
                     /locus_tag="VF531_15255"
     CDS             complement(3025..>4451)
                     /locus_tag="VF531_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715373.1"
                     /translation="LPGERPVEMDKVAAVIEKEAAKEIGPVKLNLQFYGFEDYKNQLA
                     LKASAGEPMDLIFDPSWLNFYQLQAQGAFMPIDKYIKNLSFFGKPGNVTKAVWKQVSV
                     ENKIYGLPAVENAIVYNDFASIIVRNDLLKKYAPKGIKTGTDLLAFCKAIKAKEPQYL
                     LFAQGLDNGADMASKRANSGWIYNKASLRTAPWVSLGDGDLNIMVDCSKDYSKSKTAP
                     KIFAAQFTKAWENDLWAQAQLRKQGFWSDQIIQDKRAAFKAGLAAFIGGDWSDYAMAI
                     GPAVKSTFGATWLQINRGKGYLARATGNWFCVGSKSKNPEKACAFANWIVDDRDHYEL
                     WAYGIKGEHFKINDQGALEKIDPDKRRYEMLWWYIFDESTNLRQDLSDYPDVVAYKKY
                     NSDTSHYYANPTIGFPLDMEPVKTEIAQVRAVNSEYTSFFFGQDGDSPKKLAGYRAAL
                     KKAGIDKVVAEFQKQYDAWWVKNN"
BASE COUNT         1209 a         1153 c         1016 g         1073 t
ORIGIN      
        1 attccagtat accgggcgct gaatgatata ccagatatta taacggggac gcgcccccag
       61 gcgggaaccc aacaccctgg aaccgtccgg gctttcccag acaaattcgg aacgcggcgc
      121 gatataggta ttgatgccgc ggtaaaagga aacgacgtca atgccaaacc cctgataaat
      181 ctggggtagt tgcgaaatct gcccccagcc gaagggcgag tacccggttt tgctgacccc
      241 gccgaaattt ctggcgattt tgtggcccag caacaggtta cggatgaggg attcaccccc
      301 gacgcaaaat tcgtccggca ggcagaacca cggtccaatg gctaatttac cttcacttac
      361 agccttgcga agctgatccg ccttttccgg aaaagcctcc aaataatcct gaaccggcaa
      421 ggtttgcgaa tcgagatgaa agtgtttaaa tttcggttct ttctcaaaaa tatcaaagag
      481 catttccatc agataactga gcatataacg ggtgcgttgc gccgaatacc gccattcccg
      541 atcccaatga gtattggaga taagatggca ttgaatcttt ggccggccat caacctgctc
      601 ttccttcttt actgttgtgt cagtagccat atcaattatc ctccttcagc aaatccagga
      661 tttcggtaaa attccggcag taataattgc aaagcgctcg ggtcccgctc tcgatgatat
      721 tccctccggc aaacccgaca gccggataac cggccaagcg tatggagcag atacccgccc
      781 cgctatcctc aattcctatc accgaattcc tttcgccaaa ctcgatcccc aatccgaccc
      841 ggccagtctc cgcatataac cagggatgag gtttgggcga cagttccccc agcgtgcccg
      901 cttcgccttg acggagcgga aatccggccg agatgacggc atcgtaaaaa tctttgggat
      961 cgcccatctt cagggtctga aaggcggaca atatttcagg ccatgccttt tcgtaaagac
     1021 cggaggttac caacccgatt ttgattctca tggatttcaa ttcgcataaa aaatccttca
     1081 aaccgggggt cggcacaaac gcattcttcc tgcccctgcc ttccaaaatc gcctgcattt
     1141 cccggtgggt atgttcaaaa taaaagcgcc gggcgtcctc cacctttcga ttgggacaat
     1201 atttttgaat gcaatatttt agatgctcgg atacactatg tccggaaaca taaggcaagt
     1261 cggcctcttc aagctcaaaa ccgggttttc ccaggagact ggcggtggtc atctggataa
     1321 tccaaatcca aaatgattca ctgcgtacac tggtaccgtc caggtccatt aaaacggctt
     1381 taagcggcag ttttaattcg acgggatgca ccggataata ggccgggtat cccatggttg
     1441 atttgacaag cgccacggta tgatctgtaa aggcaatgaa ctccacttta cgatccccgg
     1501 ttgcagtcac tgaagttacc ccgtttttac cggtttcaaa aataccgtcg cttgaacgcc
     1561 cgatcgctat caaaccggaa ttcccgtcga ccggcccaat cccgggaatc gtcgccaata
     1621 tttccatcct aatcctcctg tacggggaag ggtttcacaa atttaccgaa agccaatgcc
     1681 aaaagcaggg aacaaatact tccaaggaaa aacaaccatt gcagcggtga gaaatccaaa
     1741 ccaaccgcct tgggtttcag ccgctcgaat aattccaaca tagccgcacc gagattggag
     1801 gccaaaaaac ccgcaatgcc tatggaaaca ctattaatgg tcaaaaactc cgaccgcgcc
     1861 tcccctggaa tcaccgacag cagtaaacag aaacccccaa ctaaggtagc ggcatttaaa
     1921 gctccggtta acaaaaaaat caagatatac aacccatagc tattggcggt gacaaaagcc
     1981 cataatccgg atacaagggc aataccgggc aagacgtagc ggtataccga aacaaccccg
     2041 tatttatcct gtatttttcc ccatgtaaca tacagaattg tactggtgac ggtcgccagt
     2101 accgtcagtc cggatactaa aacatacggc agatgataaa ccttaaacat gatatagctg
     2161 gtatatggag tcagcattcc ctgaaaaacc gtccaaccaa aaaagaaaac catataattg
     2221 cgaaattcct tgacatgcag gcaatgcaac aggtgtcttc cgtcaatgga acgatgagac
     2281 cggtatggcg gttcgtcctg atacaagaat aaaagaatat cgaaaacgat tagcggcaaa
     2341 gcaatccaat aaagaatttg aaaaccggaa aaaccgtggc agcggtcaag cactaaaccg
     2401 gcggcaaacg gtattacgat tcccacggga ccgcccagag tataacgcag cccccagtat
     2461 gagttgcgcc ggtcaatcgg aatcagatca gccagccagt cctgataaat caggttattg
     2521 agctgtgacg ccaatgaacg caacaggatt gttcccagca aaaacgggat ccataatggc
     2581 cgcggcaata atatgggtat aaaaatgact ccccagagca aaaaacggtc aggaataatg
     2641 atcgtcatta ataatctgcg tcgcgaacgg gttttatttt gcagcccggg atatgccaat
     2701 tggattaagg tcgataacgg cgccagcgat gcaatcaaac cgagttgttg tgtatttgcc
     2761 cctaattgaa ttgcaaaagc attaaaaaat gttcccccga taagtacggt aaataatgcg
     2821 gcaataacgc cttcaatgag agtgatgcga attccatgtt ccacctgtcc ggatgagagc
     2881 ggagccggtt tttggttcag aggtcgaaat gacggtaaaa gtcccacgct tgggaatcac
     2941 catcctgtta aagcaggtat ccgtgcttcc ggtttgaacc cggaagcacg gagccgaact
     3001 aaacttactg cttcatcagc ttcatcaatt attcttaacc caccaagcat catattgttt
     3061 ttggaactcc gctaccactt tgtcgatacc ggctttcttc aatgcggcgc gatacccggc
     3121 cagttttttc ggactgtcgc catcctggcc aaagaagaag ctggtatact cggagttaac
     3181 cgctctaacc tgggcaatct cggtcttcac cggttccata tccagcggga aaccaatggt
     3241 tggattggcg taataatggc tggtatcgga gttgtatttt ttatatgcca cgacatccgg
     3301 ataatcgctt aaatcttgcc gcaggttggt tgattcatcg aaaatatacc accagagcat
     3361 ttcataacgg cgtttgtcgg gatcgatctt ttcgagggct ccctgatcat tgatcttgaa
     3421 atgttcgcct ttgatgccgt aagcccataa ctcgtaatga tcacggtcgt caacgatcca
     3481 gttggcaaag gcgcacgctt tttcaggatt tttcgacttg ctgccgacgc aaaaccagtt
     3541 gccggttgcc cgcgccagat agcctttccc acggttaatc tgcagccagg tggcgccaaa
     3601 ggtacttttg accgccgggc cgatggccat ggcatagtcg gaccagtcgc cgcctataaa
     3661 ggctgcgagt ccggctttga aagcagcccg tttatcctgg atgatttgat cgctccagaa
     3721 gccctgcttg cgcaattgcg cctgggccca cagatcgttt tcccacgctt tggtgaattg
     3781 agcggcgaaa attttgggag cagtcttgct cttgctgtaa tctttggagc aatcaaccat
     3841 gatattcaag tctccatccc ctaagctgac ccacggagcc gtgcgtaaag aggctttatt
     3901 gtaaatccag ccggaattcg cccgtttact ggccatatcc gcgccgttat ccaacccttg
     3961 ggcaaataac aaatattgcg gctctttggc tttgatggct ttacaaaacg ccaataagtc
     4021 cgtaccggtt ttaatgcctt tgggggcgta tttcttcaat aaatcgttgc gaacgatgat
     4081 ggatgcaaag tcattgtaaa cgatcgcgtt ctcaaccgca ggcaaaccgt agattttatt
     4141 ctccaccgat acctgtttcc aaacggcttt ggtgacattc ccgggttttc caaagaaaga
     4201 aaggtttttg atgtatttgt cgattggcat aaaagcgcct tgggcctgaa gttgatagaa
     4261 attcaaccag gacggatcaa agatcaggtc catgggttca ccggcggaag cctttaaggc
     4321 cagttgattt ttataatctt caaatccgta aaactggagg tttagtttaa ccggaccaat
     4381 ttccttcgca gcttcctttt caataacggc ggctacctta tccatttcca cggggcgctc
     4441 acccggcagg a
//
LOCUS       DASVMB010000543         7597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_365384,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000543 DASVMB010000000
VERSION     DASVMB010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365384"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VF531_15260"
     CDS             complement(<1..774)
                     /locus_tag="VF531_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715374.1"
                     /translation="MNKRKIYPGYFLIGVLVIYVVLVLLPSLVGIYYSFTDWNSFDPA
                     IHFVGLANFKKIFSQDELYGFYIFNTIKFTIFTTILKMIIGFGAALLLLRNVKWVNFH
                     RMVMFSPQVLSFVIVGLIFRSLLNPTEGFVNETMKMIGMGALGQNWLGDIKWAFTSIV
                     AVDTWKGAGYLMVLFIAGLKSIPDMYYEAAEIDGANGLQRLTHVTIPLIMPVISLTLI
                     LNITYGLRVFDSVYVLTNGGPGFATGVINTIVFKEFSKGT"
     gene            complement(925..2211)
                     /locus_tag="VF531_15265"
     CDS             complement(925..2211)
                     /locus_tag="VF531_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715375.1"
                     /translation="MMRKSLLILLVLVVMIGAFGVAYGAKKSVTLTFGAHQAGVPISG
                     VDQKIAKVFEKETGIKIDFQIVPDAQWRDLLKVKLASGEAPDIFLVDADPFSLNDRIH
                     PAENCIDLSKEKFISRMDPVFLPSLSYKGKIYGLSIFPGVKIWIYYYDKALFKKLNLE
                     PPKSYAEFKKVCQTIKDAGVVPVYESLANGWHQVLPLFEIGASYEKAHPKLYDELNTN
                     KAQVTDIPELLKTLTQLKEFADLGFYNKDYMTANYDDGFKVMAEGKAAMFMAGLGWRE
                     QLDQLYPGKGANIGFFIMPWADNQILNVNPAGNARFGNKKSKHVKEILQYFRFLTRHD
                     ILQMRQDLDDSTLVLNWPEIPSRLPADIQALFKNSKQGTVMQYAVKYIDSQWMDVGKD
                     IEAMYTGALTPKQVVDSIQKRRIEQAALQKDPYWVK"
     gene            2496..4388
                     /locus_tag="VF531_15270"
     CDS             2496..4388
                     /locus_tag="VF531_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7715376.1"
                     /translation="MKVRLRTIQSNLFFSYSALIIASILIFAVFFYYYTSSLLKSRVS
                     ESHQSLCESISEKLDAEVQKMNTVSMSICYSSLIKNHFYKHLSYNIKPESESNELKVE
                     KYYNAKELMDIFMAIIGPSQIVSQINLYDFNGEMVGAGISNTTARISLKKKAWYQKTL
                     AKNGDKYISAPHPDNLLARVRGINNDGIYVSLGINDDSVYISLCRVLFDRDWTKQGIV
                     EIEQDCDTIFNGLTELVNRDSGKKSIYIFSSEGNLLFPYNRKIDRRYYDYLELTRHQD
                     TPPLSVTIRNPINHTREIVTYTNSEYTGWKVLIVESQQLLLAPVVTFTKIVVLGCVGL
                     LIVTLFISFALAKKVTTPIQSIHRAIRKLDLEMLFAQTQPKLTSDLNELEELNSAFQN
                     MSVKLKQSMDALLLSQSQEMQSRMLALQSQMNPHFLYNTLATVIIMAEENMQDSVIIM
                     CKNVSHMLRYISSHKSPLVKMKAELEYTEKYLECMKFRHRSNLIYSFRIEPEMNEIRI
                     PKLLIQPLVENAIKHGTRNEPPWRIEIKALIQDHQWRVSVKDNGPGFTPEEIANINRR
                     IQDINEHYLLPSLELDGMGLLNIYIRLKLFYHEQMLFQIATPDEGGAIITIGGLPEYM
                     EAHCGE"
     gene            4378..5139
                     /locus_tag="VF531_15275"
     CDS             4378..5139
                     /locus_tag="VF531_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715377.1"
                     /translation="MENELIIKTVVAEDEELILNHIIKKIQQIDSGFVVVGAAQNGKQ
                     ALEMIEEFSPDLLLTDIRMPVIDGLELLKNVALNYPYIKTIVISGYSEFEYAKHAIKY
                     GVHDYLLKPLEPEELRKTLAKIKISLEAETDFFKHRLPKNTPGSNSPEEIMAIVIQFI
                     KENFTREINLNSLAQNFNFNASYLSKLFKKYYEETPVKYLLNLRINEAKHLLRSRPEL
                     DIKAVGELVGYPDQFYFSRIFKHMTGKSPSEFREQ"
     gene            5139..5336
                     /locus_tag="VF531_15280"
     CDS             5139..5336
                     /locus_tag="VF531_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715378.1"
                     /translation="MNKENLTSPHTAAKDAKLKLALYERVGIREFWLVQTADGMVEVL
                     PSVMTPSTENRQYMGLMARFP"
     gene            complement(5444..5947)
                     /locus_tag="VF531_15285"
     CDS             complement(5444..5947)
                     /locus_tag="VF531_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7715379.1"
                     /translation="MDYFEVIQKRHSVRSYKPDPVEREKLERVLEAARLAPTACNKQE
                     FRVIVVETAGRKDELTRIYTRDWFAEAPYVLCVCSIPSKTWVRRDNKNYADVDATIVM
                     DHIILAATAQGLGTCWIGAFDPQAAREVLKLDESLEPVAFTPLGYAKDVMVNKARKSI
                     EDLVIYR"
     gene            6081..6866
                     /locus_tag="VF531_15290"
     CDS             6081..6866
                     /locus_tag="VF531_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7715380.1"
                     /translation="MPELPDLEVFRGNLRKQLLNLPLVMIQVVNPRNVMQATGPVLDG
                     FLKQPLIAIDRNGKELFFGFPEQKCFAVHLMLNGQFHLCRSQAEVSAIPYQIAVFHFD
                     QQLLVISDPGRLCKIQFLPKPTKVPDALSPEFTLEYLEKQLIRNPLVNIKAFLIDQKM
                     VKGIGNAYADEILWESRIAPQSLCGNLTPEVIARLHGSVVKVLREAIASILQEAPDII
                     RGEVRDFLKVHNPHRKQSPTGAPIQVKKVTSKTTYFTEEQILY"
     gene            complement(6909..7424)
                     /locus_tag="VF531_15295"
     CDS             complement(6909..7424)
                     /locus_tag="VF531_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7715381.1"
                     /translation="MDVFAAINGRYSVRSYQATEVEEEKLKKVLDAGRLAPSGNNRQE
                     WKFILVRDRETRQKLATAALGQSFVAEAPAVIIACATESEHVMTCGQPAYTVDVSIAL
                     AFMLLEAYELGLGTCWLGAFNEREVKKAVGIPDSVRIVAMTPVGYPAQVTAPIKKRKS
                     FDQVVAFEGYR"
BASE COUNT         2201 a         1813 c         1541 g         2042 t
ORIGIN      
        1 agtgcccttt gaaaattctt taaaaacaat tgtattgata actccggtgg caaagcccgg
       61 accaccgttg gtcagaacat ataccgaatc aaaaaccctt aatccatagg taatattcaa
      121 tatcaaggtg aggctgatga ccggcattat caatgggatg gttacatggg tcaatcgttg
      181 taaaccattg gcgccatcaa tttccgcggc ttcgtaatac atatccggaa tcgacttcaa
      241 accggcaata aacaatacca tcagataacc ggcgcccttc caggtatcga ccgccacaat
      301 tgaggtaaaa gcccatttaa tatcccccag ccaattttgg cctaaagccc ccattccaat
      361 cattttcata gtttcattca caaaaccctc ggttggattt aacaggctgc gaaaaattaa
      421 tccgacaatc acaaatgaca aaacctgcgg ggagaacatc accatccggt gaaagttaac
      481 ccatttaaca ttcctgagta atagcaacgc agctccaaac ccgataatca tttttaaaat
      541 cgttgtaaag atagtaaact tgattgtatt aaaaatataa aacccatata attcatcttg
      601 agaaaagatt ttcttaaagt tggccaaccc cacgaaatga atcgccggat caaaactatt
      661 ccagtcggta aaggaataat aaatacctac caaactaggt agcagcacta aaacaacata
      721 aataaccaag accccgatca aaaaatagcc cggataaatt ttccgtttat tcatagttac
      781 ccctttccgt attccaggtt cctgtcgaac gctacctaat tttaaagagg cacttcaccc
      841 tttgttcagg caaagtgccc catcgatcct caccgcatca attccttacc ttatccactc
      901 taaagaatat ggaatagaat cctgttattt aacccagtac ggatcttttt gaagggcagc
      961 ttgttctata cggcgttttt gaatactatc caccacttgc ttgggtgtta aagctccggt
     1021 atacatggcc tcaatatcct tccctacatc catccactga gaatcaatat atttgactgc
     1081 atattgcatg actgtaccct gtttggaatt tttaaacagc gcctgaatat ccgcaggaag
     1141 cctggacgga atctccggcc aatttaatac caaagtggag tcatccaaat cttgacgcat
     1201 ttgtagaata tcatggcgag ttaagaaacg gaaatattga agaatttcct tgacatgttt
     1261 actcttttta ttgccgaatc gtgcgttacc cgccggatta acatttaaaa tctgattatc
     1321 agcccatggc ataataaaga atccgatatt cgcccctttt ccaggataaa gttgatccaa
     1381 ttgttcacgc cagcctagtc cggccatgaa catcgcggct ttgccctcgg ccatgacttt
     1441 gaagccgtca tcatagttag cggtcatata atctttgtta taaaatccca agtctgcaaa
     1501 ctccttgagt tgggtgaggg tctttaatag ttccgggata tcggtgactt gtgctttatt
     1561 ggtatttaac tcgtcgtaaa gtttgggatg ggctttttca taggaggcac cgatttcgaa
     1621 caagggcaag acctggtgcc aaccattggc caaactttcg tatacaggaa ccactccggc
     1681 atccttaatg gtctggcaga ccttcttaaa ctccgcgtaa ctcttgggcg gttctaaatt
     1741 taacttttta aacaacgcct tgtcataata gtaaatccag atcttcaccc cgggaaatat
     1801 tgataagcca tatattttcc ctttataaga aagactgggt agaaatacgg gatccatccg
     1861 ggagataaat ttctctttcg ataaatcaat acaattttca gcagggtgga tccggtcatt
     1921 taagctgaac ggatcagcgt caactagaaa aatatccggc gcttccccgg aggccaattt
     1981 aactttcagt aaatcgcgcc attgagcatc gggaacaatt tgaaaatcaa tcttgatacc
     2041 ggtctctttc tcaaaaacct tggcaatttt ttgatcaacc ccggagatcg ggactccggc
     2101 ctgatgggcg ccgaaggtaa gagtcacgct ctttttagcg ccataagcaa caccaaacgc
     2161 gccgatcatc actaccaata ccagtaatat caacaatgac tttctcatca tctacccccc
     2221 ctttaatctg taaccgacaa gtaaacactt aaatctgtga attggcggaa cggaaatttg
     2281 acacctccat tcaaaatgtc taaaatatta ttacgaaatc ctaaatttag attactagaa
     2341 ttagatgact tttaacatgt acaaatcgga ttttatattt gcactttttt gccaatcggg
     2401 aaaacccaga tatagacttg gtgcttagga atatttgttt taatgaaaag gcgttggatt
     2461 cctcgaaatc acccagttga acggtggttg tttttttgaa agttagactt agaaccatcc
     2521 agtccaacct gttcttctca tactcggcct taataatagc gagtatccta atctttgcgg
     2581 tcttctttta ttattacact tcctcgctgc taaaaagccg cgtctccgag tcgcaccaaa
     2641 gcctttgtga atcaatatcg gagaaactcg acgccgaagt tcaaaaaatg aatacggttt
     2701 ccatgagtat ctgttactcc agtctgatca aaaaccactt ttataaacac ttatcgtata
     2761 atatcaagcc ggagagtgaa agtaacgaat taaaagtaga gaaatattac aatgccaaag
     2821 aactgatgga tatcttcatg gccatcatcg gtccttcgca aatcgtcagt caaatcaact
     2881 tgtatgattt taacggtgag atggtaggcg caggcatctc gaatacgact gcccggattt
     2941 ccttgaagaa aaaagcttgg tatcaaaaaa cgctggcaaa aaacggtgat aaatatattt
     3001 cggccccgca tcccgataat ctactggcta gagttcgcgg tatcaacaat gacggcatat
     3061 atgtctctct aggtatcaat gatgatagtg tatatatctc cctgtgtcgg gtattatttg
     3121 acagggattg gaccaaacaa ggaattgtgg agattgaaca agattgcgat accatcttta
     3181 acggcctcac tgaactggtc aatcgtgatt ccggtaaaaa atcaatttat atttttagta
     3241 gtgaaggcaa tttgcttttc ccatataacc gcaagatcga ccggcgttac tatgattatt
     3301 tggaattgac ccgtcatcaa gacaccccac ccctatcagt aaccattcgc aatccaatca
     3361 atcatacccg ggagatcgtt acatatacca attccgaata caccggttgg aaagtcttga
     3421 tcgttgaatc tcagcagctc ctcctggctc cggtagtaac tttcaccaaa attgtagtcc
     3481 ttggttgtgt gggtttattg atagttactt tgttcatatc gtttgctctg gccaaaaaag
     3541 tgaccactcc gattcaaagc attcaccgcg ccattcgaaa attagacctg gagatgcttt
     3601 ttgctcagac tcagcccaaa ctgaccagtg atctgaacga actggaagag ttaaatagcg
     3661 cctttcaaaa tatgagcgtc aaattgaaac aatcaatgga tgctctatta ctctcccaat
     3721 ctcaggagat gcaatccaga atgctggcgt tgcaatccca gatgaaccct cattttttat
     3781 acaataccct ggctacagtg attattatgg ctgaggaaaa catgcaagac tcagtgatca
     3841 tcatgtgtaa aaacgtctcc catatgctac ggtatatctc atctcataaa tctcccctgg
     3901 taaagatgaa ggcagaactt gaatataccg aaaaatactt ggaatgtatg aaatttcgcc
     3961 accgcagcaa cctgatatat tctttccgca ttgaaccgga aatgaatgag attcgtattc
     4021 caaaactcct cattcaacca ctggttgaaa atgctattaa acatggcacc agaaatgaac
     4081 ccccctggcg aatcgagata aaggcattga ttcaagatca ccagtggcga gtttcggtta
     4141 aagacaatgg tcccggattt accccggaag aaatagcgaa tatcaaccgt cgcatacagg
     4201 atattaatga acactatcta cttcccagtc tggaacttga tggcatgggc ttgctaaata
     4261 tttatatccg gttaaaactg ttttatcatg aacagatgtt attccaaatc gccaccccgg
     4321 atgaaggggg cgcaatcatc actatcgggg ggttacccga atacatggag gcgcattgtg
     4381 gagaatgaac tgattataaa aacggtcgtg gcagaagatg aagagttaat cctaaatcac
     4441 atcattaaaa aaatccaaca aatcgattcc gggtttgtgg tcgtcggggc agcccaaaac
     4501 gggaaacaag ccttggaaat gattgaagag ttttctccgg atcttttatt aaccgatatc
     4561 cgaatgccgg ttattgatgg attggaattg ttgaaaaatg ttgccctaaa ttatccttat
     4621 atcaaaacta ttgttattag cggttacagt gaattcgaat atgccaaaca cgctatcaaa
     4681 tacggagtcc atgattacct gcttaaaccg ttggaacccg aagaactacg aaaaacgctg
     4741 gcgaaaataa aaatatcact ggaggctgag accgactttt ttaaacacag acttcccaaa
     4801 aacacaccgg gtagtaactc cccggaagag atcatggcta ttgtaatcca gttcattaag
     4861 gaaaatttca cccgggagat taacctcaat tcattagcac agaattttaa ttttaatgcc
     4921 tcatacctaa gtaaattgtt taaaaaatac tatgaagaga ccccggtgaa gtatcttcta
     4981 aacctacgga tcaatgaggc caagcatctg ttacgtagcc gtccggagct tgacatcaaa
     5041 gccgttggtg agttagtggg ttatccggat caattctatt tcagccggat ttttaaacat
     5101 atgaccggca aaagtccttc cgaattccgg gagcaataat gaataaagaa aacctcactt
     5161 ccccacatac tgcggccaaa gatgccaagc taaagttggc tttgtatgaa agggtcggaa
     5221 ttagagagtt ttggctggtg caaacggccg atgggatggt cgaagtttta ccctcggtaa
     5281 tgacgccaag tacggaaaac cggcaatata tggggttgat ggccagattc ccgtaaccat
     5341 ttttaacggg gaacgggtgg tgaatctggc cgaagtcttt gagacaacat agcttttatg
     5401 aatccgctag ccgaactcgt ttctggaatc ttgcgtttcc gtcttatcga tatataacca
     5461 gatcttcaat ggattttctg gccttattta ccataacgtc cttggcatac cctagcggtg
     5521 tgaatgcaac cggctccaaa ctttcatcaa gcttcagcac ctcccgggcg gcctggggat
     5581 caaacgctcc aatccagcaa gtccccaaac cctgggccgt agcagccaga atgatatgat
     5641 ccatcacgat ggtggcatcc acatcggcgt aatttttatt gtccctgcgt acccaggttt
     5701 tacttggaat ggagcaaacg caaagaacgt aaggggcttc ggcgaaccag tccctggtat
     5761 agatccttgt taattcgtcc ttcctgccgg cagtctcaac cacgatcacc cggaattcct
     5821 gtttattgca ggcagtcgga gccagccgcg ccgcctccag tacccgttcg agcttctctc
     5881 gctccaccgg atcgggttta tacgaccgga cactatgcct tttttggatg acttcgaagt
     5941 agtccatcaa ccaaacctcc ctttcaagat tttgaattcg gtaagtaact ttgatgatca
     6001 cctgcaggaa aacggcgatc acgacagaat tttatctata atcgaatacc caactattac
     6061 ccgcgggaag aggtgtaatc atgccggagc tgccggattt ggaagtattc cgtggcaatt
     6121 taagaaaaca gttgcttaac cttcctttgg tgatgatcca ggtcgttaat cccagaaatg
     6181 tgatgcaggc caccgggccg gtgctggacg gatttctaaa acaacccctg atcgcgattg
     6241 accggaacgg caaagagtta ttctttggtt tcccggaaca aaagtgcttc gccgttcatc
     6301 tcatgttaaa cggccagttt catttgtgcc ggagccaagc cgaagtatcc gccattccct
     6361 atcaaatcgc agtttttcac tttgaccaac aactgctggt gatcagcgat ccgggccggt
     6421 tgtgcaaaat tcagttcctg cccaagccta ccaaagtccc ggatgccttg agcccggaat
     6481 ttacccttga atacctggag aagcaattga tccggaaccc gctggtgaac atcaaggctt
     6541 ttttgatcga ccaaaaaatg gtcaaaggaa tcggcaatgc ctatgccgat gaaatattat
     6601 gggaaagccg gattgcgccg caatcgctct gcggcaattt aactcccgaa gttattgcgc
     6661 gtcttcacgg aagcgtcgtc aaggttttgc gagaggcgat cgccagcatc ttgcaagaag
     6721 cgccggacat catccgcggc gaggtccggg actttctgaa agtccataac ccgcaccgta
     6781 aacaatcgcc caccggtgcg cccattcagg taaaaaaagt gacgtccaaa accacgtatt
     6841 ttacggaaga acaaattctt tattaagctg tacaacggtt gatacaattg gctcaaatgg
     6901 gggaaatcct accggtatcc ttcaaaagcc accacttgat caaagctttt cctctttttg
     6961 atcggcgcgg tgacttgcgc cggataacca acgggcgtca tggccacgat ccggaccgaa
     7021 tcggggatgc cgacggcttt cttgacttca cgctcattaa atgcacccag ccaacaggtt
     7081 cccagcccca attcataagc ttccaataac ataaacgcga gcgcgatgga tacatccacc
     7141 gtataggcgg gttggccgca cgtcatcacg tgctccgact ccgtagcgca ggcaatgatc
     7201 actgcgggag cctccgccac aaaggactgg ccgagagcgg ccgtagccag cttttgacgg
     7261 gtttccctat cccgtaccaa aatgaatttc cactcttgtc ggttattgcc tgaaggcgcc
     7321 agccgtcctg catccaagac cttttttagc ttttcttctt caacctcagt tgcttgatag
     7381 gatcttacac tgtatcttcc attgattgcc gcaaatacat ccattttttc tcctccagtc
     7441 gttcccgatt cataccgccg aactatacat gggattaccc ggcccagcat gaccgggata
     7501 tttaggcttc ttccctatga atctgtttta atagtttagc catgttccgg ccgagggtct
     7561 cgaaggtccg gatcccctcc tcatccttct ggacatc
//
LOCUS       DASVMB010000544         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_290438,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000544 DASVMB010000000
VERSION     DASVMB010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290438"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VF531_15300"
     CDS             complement(<1..1006)
                     /locus_tag="VF531_15300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7715382.1"
                     /translation="MRFATILLGCKLGGLLLAGLILRFWIRLAPKPGDPDFTAKRWRH
                     LIGLRFLYPLLFVVYGWILLKYPLMVVAQRRLTIAAHWIDDVLNLSGLTVYMAYFLHL
                     SYSLERSYGYTGFGFGSFLAQNGYTQLMILGGLIFLRLDITYLPILSNPGVWYFNPIF
                     LEAVLVVLFIFFQLFVLRLRLMRMEPSGPDLRNLVNGVADRVGVKIKFLRVWRWDGMI
                     NAYAGGVLFPSVFLTRLLVATASPQDLQMVVGHECAHLKQRHPLIRMVYIAVMTYFGS
                     WLWDALSAHNRIWIAGFALLAFLGYQALARFQEFQADALSARMLGGERPMIEALVRIF
                     D"
     gene            complement(1003..1656)
                     /locus_tag="VF531_15305"
     CDS             complement(1003..1656)
                     /locus_tag="VF531_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7715383.1"
                     /translation="MYQAIVFDLGNVLLEISFKRVCRYWADITGRDPQELYASFPRDE
                     IFTGFEKGEISPEIFRRHVMTKMGCSMNTMEFDNGWNAIFVGPVPGIESVLVQLKKRY
                     RLVGLSNTNPIHAAWWQAPYGALVARLERLFVSHEIGTRKPEPRAYEIVLDYLGLRPE
                     EVIFIDDTEENVIASEQLGIKGLVMQTTPKLVRDLRELGIPITLPPDGEIFSKRSSA"
     gene            complement(1778..2662)
                     /gene="folD"
                     /locus_tag="VF531_15310"
     CDS             complement(1778..2662)
                     /gene="folD"
                     /locus_tag="VF531_15310"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX7715384.1"
                     /translation="MTARIIDGKAVAARVKEELKQRVDGLITGGTRPGLAVVIVGSDP
                     ASRIYVNNKKKASAELGIYSEEHALPERTTEAELLQLVDDLNSNPEIHGILVQLPLPE
                     QINERLVLERIDPAKDVDGFHPVNLGNLLSGLPGFLPCTPAGVMELIYQTGVDITGKE
                     CVVVGRSNIVGKPQAILLLREDGTVTICHSRTRNLEAICRRADILVVAVGKPEFITGD
                     MIKPGAVVIDVGMNRNSEGKLCGDVQFSTAAEVAGWITPVPGGVGPMTIAMLMRNTVE
                     SATRLKYKGIGNRQQAKV"
BASE COUNT          733 a          773 c          640 g          614 t
ORIGIN      
        1 tatcgaagat ccggaccagc gcttcgatca tcggccgctc ccctcccagc atccgggctg
       61 atagtgcatc ggcctgaaac tcctgaaaac gcgctaaggc ttggtaaccc aggaaagcaa
      121 ggagagcaaa accagcgatc catatcctgt tatgggcaga caacgcatcc cagagccacg
      181 agccaaaata agtcatgacg gcaatgtaca ccattcggat cagcggatgg cgctgcttga
      241 gatgggcgca ttcgtgaccc acaaccattt gcaggtcctg aggcgatgcg gttgccacca
      301 aaagtcgggt caaaaaaaca ctcggaaata agactcctcc ggcataggcg ttgatcattc
      361 cgtcccagcg ccaaacccgg aggaacttta ttttgacccc aacccgatcc gccactccat
      421 tcaccaggtt tctcaggtcc ggtccggatg gctccatccg catcaatcgc agccgcagga
      481 cgaatagctg aaagaagata aacagtacaa ccagaacggc ttccaaaaat ataggattaa
      541 agtaccagac accaggattt gaaaggatgg gaagatacgt gatgtcgagt cgtaaaaaga
      601 tcaatccacc gaggatcatc agctgggtat aaccgttctg ggctaaaaaa gaaccgaaac
      661 caaagcctgt ataaccgtaa ctccgctcga gactatatga cagatgcaaa aagtatgcca
      721 tataaacggt aaggccggac aagttcagga catcgtcgat ccagtgagcg gcaatggtca
      781 agcggcgttg tgcgactacc atcagcggat atttcaacaa tatccatcca taaacgacaa
      841 acagcagtgg atacaaaaaa cgcagcccga ttaggtggcg ccaccgttta gcggtaaaat
      901 ccgggtctcc cggcttcgga gcgagccgaa tccaaaatcg gagtatcaac ccggcgagta
      961 acagaccacc tagtttgcac ccgagcaaaa tcgtcgcgaa tctcacgccg aagacctctt
     1021 tgagaaaatt tctccgtccg gaggtaaggt gatgggtatc cccaattccc ggagatcacg
     1081 cacaagttta ggggttgtct gcattaccaa acccttgatg cccagttgct cggaagcgat
     1141 gacattctct tccgtatcgt cgatgaagat cacttcctcc ggccggagcc caaggtaatc
     1201 aaggacgatt tcatacgccc ggggttcagg tttacgcgtt ccaatctcgt gggagacgaa
     1261 gagacgttcc agccttgcga cgagggctcc gtatggagct tgccaccagg cggcatgaat
     1321 cggattggta tttgaaagtc ccaccagacg gtatctcttt ttcaattgaa cgagaacaga
     1381 ctcaatcccc ggtacggggc ctacaaaaat ggcgttccaa ccattgtcga actccatggt
     1441 attcatggag catcccattt tcgtcatcac atgacgcctg aaaatttccg gactgatctc
     1501 ccctttttca aatccggtaa aaatctcgtc ccggggaaaa ctcgcataaa gctcctgagg
     1561 atcgcggccg gtgatatccg cccaataccg gcagaccctt ttaaatgaga tctctaaaag
     1621 tacattgccc agatcaaaga cgatggcttg gtacatgaat gagactcctt acattagtga
     1681 taggcaacag gcaattggct ataggcaaca gtattattga gtaatagaaa taggtattaa
     1741 gcaggacttt tcgaagttat aaaacagaac gtagctttca gacttttgcc tgttgcctgt
     1801 tgcctattcc cttatacttt agacgagtgg cgctctccac ggtattccgc atcaacatgg
     1861 cgatggtcat gggcccgacc ccaccgggaa ccggtgtgat ccaccccgcg acctcggctg
     1921 cagttgaaaa ctgaacgtca ccgcagagtt tcccttcgga gttccggttc atgccgacat
     1981 cgatcaccac ggcgccgggt ttaatcatat ccccggtgat gaattccggt ttcccgactg
     2041 caacgactag tatatcggcc cgcctgcaga tggcctccag gtttctggtc cgggaatgac
     2101 agatcgtcac cgtgccgtcc tcccgcagca gcaatatggc ctgaggtttg cccacaatat
     2161 tactcctgcc caccaccacg cattctttac ctgtgatatc cacaccggtc tggtagatca
     2221 actccattac gcccgccggg gtacagggca gaaatccggg taagcccgac aagagattgc
     2281 ctaaattgac cggatgaaaa ccatccacat ctttcgcagg atcaatccgt tccagaacca
     2341 accgttcatt gatctgctcc ggcagaggca gttgaaccag aatcccgtgt atctccggat
     2401 tgctgtttaa atcatccacc aattgtaaca gttcagcctc ggtagtccgc tccggcaagg
     2461 cgtgttcttc agagtaaatg cccagttcgg cactggcctt ttttttatta ttcacgtaaa
     2521 tccgggaagc aggatcactc cccacgatca ctacggccag ccccggcctt gtaccgccag
     2581 tgatcaaacc atccacccgt tgtttcaatt cctcttttac ccgggcggca accgcctttc
     2641 cgtcaatgat tcgggccgtc actatatcac tcctaagctt ccagtttttc accacgaaaa
     2701 gcatcgatat aacggagagc gtattcatcc cgttccagta acacttccgt agcgtaaatc
//
LOCUS       DASVMB010000545         5724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_213796,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000545 DASVMB010000000
VERSION     DASVMB010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213796"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF531_15315"
     CDS             <1..181
                     /locus_tag="VF531_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715385.1"
                     /translation="LIASIASIVTPPIRGLFRFFHDCRVSALEPVDPNIIHSHNPWQF
                     LYEYLPYSQEICGER"
     gene            complement(151..1704)
                     /locus_tag="VF531_15320"
     CDS             complement(151..1704)
                     /locus_tag="VF531_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7715386.1"
                     /translation="MTIISTSFASTPGSAAKPDSPNPQACLANLIPKPESVVPGNGAF
                     CLTPETKIFICPENLELKGIGNFLAERLYPATGLKPAVVPISASAPIPEGNIYLAMAE
                     EDSTLGEEGYRLKITAKQVTLNACRPAGIFHGVQTLRQLLPPTVELATLQPGPWNLAA
                     VTITDRPRFAWRGAMLDLARHFFDAGEVKRYIDRLAYYKLNRLHLHLTDDQGWRIAIQ
                     SWPNLALHGGSTAVGGDRGGYFTAEAYKDIVSYAQSRYITVVPEIDMPGHTNAALASY
                     PELNKNGVAPPLYTGIKVGFSTLAFNKEVTYKFLDDVIGELAALTPGPYLHIGGDEAL
                     TVSKEDYTRFIERVQEITQTHGKQAVGWEEVAAAKLLPTTVVQPWKDEKMARAAAAKG
                     AKVILSPANKLYLDMKYTPKTGLGQNWSGYIEVKESYDWDPANLVGGVAEQAILGVEA
                     PLWTETIRTYADVEYMVFPRIIGCAEIGWSSKDGRNWTEYARRLASHGPRLTAMEVNF
                     YRSPQISWE"
     gene            complement(1957..3432)
                     /locus_tag="VF531_15325"
     CDS             complement(1957..3432)
                     /locus_tag="VF531_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7715387.1"
                     /translation="MTTGSIRTRLFLGVTSLTIFFVLFSWFLNINFLDKYYIAQKGAT
                     LRKAYSHINGIYDGDIGAARSDLEKMVHTTGINIVILDRDFVIKYDSAPRPRGLPPRP
                     APGEHRVPRFISQSADWSQPTYRVDRTHNPANTEFLNFTAKLQNGDYLWLGMPLAEIQ
                     QGVAISNQFFLLTGLLTILIGGIIVLFYSKRFTRPILELNTIAQSMAKLDFTKMYPVK
                     SRDEIGELGRSINSLSEQLGRSIRELQQANRQLEEDNERQKKIDEIRKEFISNVSHEL
                     KTPIALIQGYSEGLKLNVIENEADKNFYCNVIMDEAAKMNKLVRELLDLSEIDSGFLR
                     LDMESFDLAQLTEHILEKYHLLLKEKEIRLGFEADHPAWVRADISRIEQVLANYIDNA
                     LGHIEAEKLLRVGIRVRGGRVRVAVFNSGQPIPEEAVEKIWMSFYKVDQARTRAYGGT
                     GLGLSIVRAIMERHQGGFGVRNLEGGVEFWFELEQVVPDEA"
     gene            complement(3429..4115)
                     /locus_tag="VF531_15330"
     CDS             complement(3429..4115)
                     /locus_tag="VF531_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715388.1"
                     /translation="MNERTILVADDEDRMRKLVGDFLKKAGFRIIEAEDGKKALQVFK
                     EVPRIDLVILDVMMPALDGWTVCREIRKISKVPVIMLTARGEEFDQLFGFDLGADEYV
                     TKPFSPSVLVARVQALLRLVEGTDKAKTVMDYAGLQIDKDGHSVAVDGNPVDLSPKEY
                     ELLLYITENEGVALSREQILNAVWNYDYFGDARTVDTHIKKLRLKLGARGDYIQTVRG
                     FGYRFEVKNP"
     gene            complement(4241..5440)
                     /locus_tag="VF531_15335"
     CDS             complement(4241..5440)
                     /locus_tag="VF531_15335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715389.1"
                     /translation="MKVSENLNMAVKNLAANKIRSLLTMLGVIIGVAAVIDMVALGEG
                     AKRQVTSNISAMGSNLVMVFSGMGSRQGGSFGGGQPLTNDILPTIEYASSKITLIAPE
                     ASRNLVVGNNDSSMVSSVVGCTLPYFKMRNYQVAQGRTFSESELNARSRVAVIGSYLA
                     EQLFSGSDPVGQDLKVGGIFRFQIVGVLKEKGQNGASNSDNMVLIPLATAQQRIFGNR
                     NLSTIYVQADSDQDVSMVYNGVYTALLAKFKDESKFTLRNQAEILSTVEQTTKTFTLL
                     LAGIAAVSLLVGGIGIMNIMLVSVTERIKEIGVRKAIGAEKSEILTLFLIEAVGLSLM
                     GGLIGIILGWILAALISHFSGWATVISLNSILISFGFSIMVGLFFGGYPAYKAAGLHP
                     IEALRHE"
     gene            complement(5437..>5724)
                     /locus_tag="VF531_15340"
     CDS             complement(5437..>5724)
                     /locus_tag="VF531_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7715390.1"
                     /translation="ELSGGQQQRVAIARALVNRPKLLLADEPTGNLDSKASVEIMAIF
                     QELHCEGMTLVLVTHENDIAGFTQRIIRFCDGKIVADESNRQVIIKKEVPA"
BASE COUNT         1370 a         1649 c         1324 g         1381 t
ORIGIN      
        1 cttgatagcg tcgatcgcat ccattgtcac tcccccaatt cgcggcctat tccggttttt
       61 tcacgactgc cgggtatccg cccttgagcc ggtagacccg aacataatcc acagccataa
      121 cccatggcaa ttcctttatg aatacctccc ctactcccag gaaatctgcg gtgagcggta
      181 aaaattaacc tccattgccg tcaagcgcgg tccatggctg gccagacggc gggcatactc
      241 cgtccaattt cttccatcct ttgatgacca tccaatctcc gcacaaccga tgatccgcgg
      301 gaagaccata tattcgacat ctgcgtatgt tcggatggtc tccgtccaga gcggagcctc
      361 cacccccaga atggcttgtt ccgcaacccc accaacgagg ttagccggat cccagtcata
      421 actctccttg acctcgatat aaccggacca attctgcccc agaccggttt tcggagtata
      481 cttcatatcc aggtatagct tgttggcggg cgacaggatc actttcgccc ccttagccgc
      541 tgcggcccgg gccatcttct catccttcca gggttgaacg acagttgtcg gcaaaagctt
      601 cgctgcagca acctcctccc atccaaccgc ctgtttcccg tgggtttgtg taatttcttg
      661 gacccgttcg atgaagcggg tataatcctc cttggagacg gtcaacgcct cgtcaccacc
      721 gatatgcaag taaggcccgg gagtgagggc agctaactcc ccgatgacat catccagaaa
      781 cttgtaggtg acctctttat taaacgccag ggtgctgaag ccgaccttga tccccgtata
      841 aagcggaggt gccaccccgt ttttgttcaa ctcgggataa gaggcgaggg ctgcattggt
      901 atgccccggc atgtcgatct ccggaaccac cgtgatatat cggctttggg cgtaggagac
      961 gatatcctta tacgcttccg ctgtaaaata cccgccccga tccccgccca ccgcagtact
     1021 cccgccgtgc agcgccaggt tcggccagga ctgaatggcg attcgccagc cctgatcgtc
     1081 cgtgaggtgc agatgcagcc gatttagttt atagtaagcc aatcgatcaa tatagcgttt
     1141 cacttccccg gcgtcaaaaa aatgcctggc cagatcgagc atggcaccgc gccatgcaaa
     1201 acgcggccgg tcggtaatgg taaccgccgc aaggttccag gggccgggct ggagcgtagc
     1261 aagctcaacg gtaggcggca ggagttgccg gagagtctga acaccgtgga aaattcctgc
     1321 cggacgacag gcgttgagcg ttacctgttt cgcagtaatc ttgagccggt aaccctcctc
     1381 accaagggtg gaatcctctt cggccatcgc aagatagata tttccctcgg gaatgggggc
     1441 ggaggcagaa atcggaacta ccgccggctt taatcctgtg gccggataca accgttcggc
     1501 caagaaattc ccaattccct tgagttcgag gttttcggga caaatgaaaa tcttcgtctc
     1561 cggtgtcagg caaaaagcgc cgttacccgg taccaccgat tccggcttgg gaatcagatt
     1621 ggccaagcag gcctggggat tgggggaatc gggcttggcg gccgatcccg gggtactggc
     1681 aaatgatgtg ctgattatag tcatgataag tagtaaacct acgaacctcc tccattgaaa
     1741 tggggtttga cagagcatga gcgttctccc ccttattcat ttgactaacc gtttcaccaa
     1801 tgatccatat ggatccgtgt tgtgatttcc acctgctgtt ggggtatcac cttcgcttcc
     1861 ttttccttaa ccatcaactc catacatatg cctccttgga atcatttcat ccactccatc
     1921 atgaaaacaa ggaaatcaat gtttcccgta aaaatattac gcttcatccg gcacaacctg
     1981 ctccagctca aaccagaact ccacgccgcc ctccaagtta cgaactccaa aaccgccttg
     2041 gtgccgttcc ataatcgccc ggacgatgga gagccccaat ccggttccgc cgtatgctct
     2101 ggtccgtgcc tgatcgacct tgtaaaaact catccaaatc ttctcgaccg cttcttcggg
     2161 aatgggttgg ccggaattaa agactgcaac ccggaccctc ccgccccgga cccggattcc
     2221 gacccgtaac agcttctctg cttcgatatg gcctaacgca ttatcgatat aattcgccag
     2281 cacctgctcg atccgggaga tatcggccct tacccaagca ggatgatccg cctcaaatcc
     2341 cagacggatt tctttttcct taagcagtaa atgatacttc tccaaaatat gctccgtgag
     2401 ttgggccaga tcgaaacttt ccatatccag ccttaaaaat ccggagtcga tctccgaaag
     2461 gtccagtaac tcccggacca gtttattcat tttggcggct tcgtccataa tcacattgca
     2521 gtaaaagttt ttatccgcct cattctcaat aacattgagt ttaagtccct ccgaataccc
     2581 ttggatcagg gcaatcggtg tttttaattc atgagaaaca ttggagatga attccttccg
     2641 gatctcgtca atcttctttt gacgctcgtt atcttcttcc aattgccgat tggcctgttg
     2701 caactcgcga atggaccggc ccaattgttc cgaaagcgag ttgatgctcc ggcccaattc
     2761 tccgatctcg tcccgcgact tgacaggata catcttggta aagtcgagct tcgccatact
     2821 ttgagcgatg gtgttcaact ccaatatcgg acgcgtaaac cgcttggaat aaaataagac
     2881 aataatgccg ccgatgagaa tcgtcaataa tccggtcagt aagaaaaatt ggtttgatat
     2941 tgccacgccc tgttgaatct cggccaacgg catacccaac cacaagtaat cgccattttg
     3001 cagcttggcg gtaaagttca aaaactcggt attggcagga ttgtgggtgc gatcaacccg
     3061 ataggtcggt tgcgaccagt cggcgctctg ggaaataaag cgcggtaccc ggtgctctcc
     3121 cggagccggc cgcggtggca aaccccgggg ccggggggcc gagtcatact tgatgacaaa
     3181 atcgcgatcc aggatcacaa tattgatccc cgtagtatgc accatttttt ccagatcgga
     3241 gcgggcggcg ccgatatcac catcgtatat cccgttgata tgactgtatg ccttgcgcaa
     3301 agtcgcccct ttttgggcga tataatattt gtcgaggaaa ttaatattca ggaaccagga
     3361 aaagaggaca aaaaagatgg tcagcgaggt gactcccagg aaaagcctgg tccgaatcga
     3421 gccggtagtc atggattctt cacctcaaac cgatacccga aaccgcgtac cgtttggata
     3481 tagtcgcctc tcgccccgag tttcaggcgg agttttttga tatgggtatc tacggtacgg
     3541 gcatccccga aataatcata gttccacacc gcgttcagga tctgctcccg gcttagcgca
     3601 actccctcgt tctcggtaat atacagtagc aactcgtatt ccttggggct cagatcgacg
     3661 ggattgccgt caaccgccac gctatgcccg tctttatcaa tctgcaaccc ggcatagtcc
     3721 atcacggttt ttgccttgtc agtcccctca accaaacgga gtaaggcttg aacccgcgcc
     3781 accaaaacgc tggggctgaa cggtttggtg acgtattcgt cggcacccag gtcgaatccg
     3841 aagagctggt cgaattcctc cccccgggcg gtgagcataa tcaccggtac tttagatatc
     3901 ttgcgaatct cccggcagac cgtccaaccg tctaacgcag gcatcatcac atccaggatc
     3961 accaggtcaa tccggggaac ctccttaaag acttggagag ctttcttgcc gtcctccgcc
     4021 tcaatgatcc ggaaacccgc ctttttcagg aaatcgccga ccagtttccg catccggtct
     4081 tcgtcatcgg cgaccaatat cgttctctcg ttcatcatcg gctgctcctt cgcctccgcg
     4141 atagactctt aataacttct ccgtcccgca tcagactcct gcgggacgga aggtttcaag
     4201 acaaattagt tcaaattagt tcgtgcccat aggtgtcaaa tcattcatga cgcaatgctt
     4261 cgattggatg caaacccgcg gctttatatg ccggataacc tccaaaaaag agcccgacca
     4321 tgatggagaa cccaaatgag atcaagatgg agttgagtga gatcaccgta gcccaaccgg
     4381 aaaaatgaga aatcagcgcg gccagtatcc atccgagaat gattccgatc aaccctccca
     4441 taaggctcaa tcccacggct tcaattaaaa acagcgtcag aatctccgac ttctccgccc
     4501 cgatcgcctt acggactccg atctccttga tcctttcggt aaccgaaacc agcataatgt
     4561 tcatgatccc gataccgccg accagtaatg agaccgcagc gattcctgcc aagagcagcg
     4621 taaaggtttt tgttgtctgc tcaacggtgg agaggatctc cgcctgattc ctgagggtaa
     4681 acttggattc atccttgaac tttgccaata aggccgtgta aactccattg taaaccatac
     4741 tcacatcttg atcgctgtcg gcttgaacgt aaatagtgct taaattacga ttgccgaaga
     4801 tccgctgctg cgcagttgcc aaaggtatca aaaccatatt atcactgttg ctggcaccgt
     4861 tttggccttt ctcctttaaa acgcctacaa tttgaaagcg gaagatgccc cccactttca
     4921 ggtcctgccc aaccggatcg gagcctgaga acaactgctc agccaggtag gaaccgatga
     4981 ccgccacccg gctcctggca ttaagctcgg attcactgaa agtccggcct tgtgccactt
     5041 gataattacg cattttaaaa taaggaaggg tacagcccac caccgaactt accatgctgc
     5101 tgtcgttatt ccccaccacc aaattgcggc tggcttcggg agcaatcaag gtgattttcg
     5161 aagaagcata ttcaatcgtg ggtaagatat cattcgtcaa cggctgaccg cctccaaacg
     5221 agcctccctg gcggctgccc attcccgaaa acaccatgac caggttggaa cccatagcgc
     5281 tgatattcga cgtaacctgc cgttttgctc cctcacccag ggctaccata tcaataactg
     5341 cggccactcc aataattaca cccaacatgg tcagtaagga acggatttta ttcgccgcca
     5401 gattcttcac tgccatattt aaattctcgg atactttcat gccggcacct cctttttaat
     5461 gatgacctgc cggttggatt catctgctac aatctttccg tcgcagaaac ggataattcg
     5521 ttgtgtaaaa ccggcaatgt cattttcatg agtcaccagc acaagtgtca ttccttcgca
     5581 gtgcagttcc tggaagatgg ccatgatttc cacgctagcc ttggaatcca gattaccggt
     5641 gggttcatca gccagtaaca gcttggggcg gtttaccaga gcccgggcaa ttgccacccg
     5701 ctgctgttga ccgccggaga gctc
//
LOCUS       DASVMB010000546         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_365555,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000546 DASVMB010000000
VERSION     DASVMB010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365555"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1178)
                     /locus_tag="VF531_15345"
     CDS             complement(105..1178)
                     /locus_tag="VF531_15345"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX7715391.1"
                     /translation="MINQKHNKDFLPKWIIDEGEMGDVVLSSRIRLARNLKGIPFPGC
                     ANEQQLAEVNTLVHQAIKSDSPLGPYAVINLEELPPNDRVVLVEKHLCSPQFIETPHL
                     HSLIVNQPQTVSVMINEEDHLRIQAIVAGFAVGQALELANQVDDFLEESLEYSFDETY
                     GYLTTCPTNVGTGIRASVMMHLPGLTMVDQVKRVLSALTHIGINIRGIYGEGTESFGD
                     LYQVSNQVTLGFTEQDLANNLTSVCKQVIEQERAVREALLKDSKMQLEDRLYRSYGIL
                     TQARVINTQEALKLLSDVKLGVDIGMIRGIDRSVIRELILSIRASILQKVIGKELAPA
                     DRDYYRAELIRERLKGGLKVPEK"
     gene            complement(1191..1709)
                     /locus_tag="VF531_15350"
     CDS             complement(1191..1709)
                     /locus_tag="VF531_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX7715392.1"
                     /translation="MSSLLCERCHERPATVHLTKIINFQKTEFHLCEVCAKSAGSELG
                     FIFEPTFTFQNLIAGLLEGDTGIYQQPATVKELYCPNCGLTFTDFRNNGLLGCADCYN
                     NFQSGLEPLLKRVQGNTRHIGKVPKRSGGKVRIKKEIADLRQELQQSIGREDYERAAE
                     LRDEIRRREQDL"
     gene            complement(1824..2282)
                     /locus_tag="VF531_15355"
     CDS             complement(1824..2282)
                     /locus_tag="VF531_15355"
                     /inference="COORDINATES: protein motif:HMM:NF017648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEX7715393.1"
                     /translation="MSNLAELIEQYLRRMLMQQKIIELQRRELARMFRCAPSQINYVL
                     ETRFTFEKGYIIESKRGGGGYIRITRIQWPTEESLPELMEQFVPDELSEDDALMILQE
                     LVERKMINLQDAQLAGKLMDREFSDLPDEYADSLRARFLKALLLMMGSKE"
     gene            complement(2475..>2901)
                     /locus_tag="VF531_15360"
     CDS             complement(2475..>2901)
                     /locus_tag="VF531_15360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7715394.1"
                     /translation="IIFVPRELPNRAQYSAFPNLIFPVRGLISSRYGFRWGRMHYGLD
                     LAAPTGTRVYAAASGKVIFAGWLGSYGLLVKIDHGQYVTYYGHLSRILVNRGERVGQG
                     DPIARVGSTGRAYGSHLHFEVERNGRKLNPVQFLARRDM"
BASE COUNT          707 a          792 c          644 g          758 t
ORIGIN      
        1 tatgtccaag gcgtgctgcc tcctgttgcg caagatatac aacctttctg gccctttccg
       61 taaaacgttc gaacattttg gttcacctcg tttagataag acgtttattt ttccggtact
      121 tttagaccgc cttttaaccg ctcccgaatc aattccgccc ggtagtaatc tcgatcggcc
      181 ggggcaagtt ccttaccgat gaccttctga aggatcgacg cccggatcga caggattaac
      241 tcccggatca cggaacgatc aatcccccga atcatcccga tatccacgcc caatttcaca
      301 tcagaaagaa gtttcaacgc ctcctgggtg ttgatcaccc gggcttgagt caatattcca
      361 tacgaacgat aaagacgatc ctcaagctgc atcttggagt ctttgagtag agcctcacga
      421 accgcccgtt cctgttcgat cacttgtttg cagacactcg tcaggttatt tgccagatcc
      481 tgctcggtaa atcccagggt tacctgattg gaaacctggt aaagatcgcc gaaggattca
      541 gtgccctcac cgtatattcc ccggatattg atgccgatat gtgttaatgc cgacaagacc
      601 cgtttcacct gatccaccat tgtaagcccg ggtaaatgca tcattaccga ggctcggatc
      661 ccggttccca cgttcgtggg gcaagtggtt aggtatccat aagtttcatc aaaactatat
      721 tccagtgact cctccagaaa atcatcaacc tggttggcaa gctccagggc ttgaccaacg
      781 gcgaaacctg ccacaatggc ctggattctc agatgatcct cctcattgat catgacgctg
      841 acggtctgag gctggttcac gatcaagcta tgcagatgag gtgtttcgat aaactgtggg
      901 ctgcagagat gtttctccac caaaaccaca cgatcgttag gagggagttc ctccagattg
      961 atcactgcat acgggcccag cggtgagtcc gacttaattg cctgatgaac cagggtgttg
     1021 acctcggcta attgttgttc atttgcacag ccgggaaagg gtattccttt gaggtttcga
     1081 gccagacgaa tccggctgct cagcacgaca tcgcccatct ctccctcgtc gatgatccat
     1141 tttggtaaaa aatccttatt atgcttttga ttgatcaaaa agcgcacccc ctatagatcc
     1201 tgctcacggc ggcggatctc gtcgcgcaat tccgcggcgc gttcgtaatc ttccctgccg
     1261 atggactgct gcaactcttg ccgtaaatcg gcaatctcct tcttgatccg aactttacca
     1321 ccactgcgtt tgggcacttt tccgatatgg cgggtgttac cctgtacacg cttgagtaat
     1381 ggttccaaac cgctctgaaa attgttgtaa caatcggcac agcccaaaag accattattt
     1441 ctaaaatcag taaacgtcag accacagttt ggacaataaa gctctttcac agtggccggt
     1501 tgctgatata tgccggtatc tccttcgagc agtccggcaa tgagattctg aaaggtaaag
     1561 gtcggttcaa aaataaagcc caattcgctc ccggcgctct tggcgcagac ctcgcagaga
     1621 tgaaattcgg tcttctgaaa attaatgatc ttggtcaaat gaaccgttgc cggcctttca
     1681 tgacatcgtt cacataacaa gctgctcacc ccgtttcaaa ttttataatt caatgttggt
     1741 ttgacatccg ataaagtcag tatattatat aacttttccg gtggcgatag cctacctgta
     1801 aatactatct cgcaggtgca aattcattcc ttgctcccca tcattagcaa caaagccttt
     1861 aaaaatctag cccgcaagct gtcggcgtac tcatcaggga gatcggaaaa ttcccggtcc
     1921 attaatttac ctgccaattg agcatcctgg aggttgatca ttttccgttc caccaattcc
     1981 tgaaggatca tcagcgcatc atcctccgaa agctcatcgg ggacgaactg ttccatcagt
     2041 tccgggaggc tctcctcggt cggccactgt atccgggtga tccggatgta tcccccgccg
     2101 ccgcgtttac tctcgatgat gtaacccttc tcaaaggtaa aacgggtctc aagcacataa
     2161 ttgatctgcg atggagcgca gcggaacatt ctggccagct ctctccgctg taactcgata
     2221 attttttgtt gcatcagcat ccgccgcagg tattgttcta tgagttcagc caggtttgac
     2281 attgtttcac ctctgacctt atctgacctt attgtaaata atccctcttc aaaaaacaag
     2341 ggaaggttta cagaagctcc tccccaaaaa taaccgttat tcaaatttaa gtcaccaata
     2401 ctcgctcttt tccatgtttt cgctgctttt cacgacgaat gatttattga ctcaccgcgt
     2461 acgcgcgaat catcttacat gtcgcggcgg gccaaaaact gaaccggatt caacttgcgc
     2521 ccgttgcgtt cgacttcaaa atgaagatga ctgccgtacg cccgtccggt tgagcctacg
     2581 cgggcgatgg gatcgccctg ccccacccgt tcgccccggt tgaccaggat ccgcgagaga
     2641 tgcccataat aggtaacata ttggccatga tcgatcttga ccaataatcc gtatgaaccg
     2701 agccagcccg caaagatcac ttttccggat gcggcggcat atacccgtgt tccggtgggt
     2761 gccgccaggt ccaacccata atgcatccgg ccccagcgga atccataacg ggaactgatg
     2821 agcccccgca ccggaaagat cagatttgga aaagcggagt attgagcacg gttgggcagt
     2881 tcacgcggta cgaatattat c
//
LOCUS       DASVMB010000547         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_213847,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000547 DASVMB010000000
VERSION     DASVMB010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213847"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..962
                     /locus_tag="VF531_15365"
     CDS             426..962
                     /locus_tag="VF531_15365"
                     /inference="COORDINATES: protein motif:HMM:NF028409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HEX7715395.1"
                     /translation="MSGNVETRVVKHQGLQAIDLAVAALLIAAGAVIRLTVPPMFGIT
                     PNFAIAMYSLAIILARPRLGEAIGIGVVAGLVCMFTSKAAYPVNLISEPIGAMVCLGI
                     FSLLEAKFKGFVLTPLLTGALATLASGLSFVAVYVVFKLVPLAVAANMLITVVTLTVL
                     NAIIVQVLYFPAKKLLKK"
     gene            1007..1918
                     /locus_tag="VF531_15370"
     CDS             1007..1918
                     /locus_tag="VF531_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715396.1"
                     /translation="MTLFQQEPLAKKEEPLIAARNLSFSYHDSPTPALREINLTVSKG
                     EFIGITGPTGAGKTTLAHCLNGVIPHFVTGDYFGDVAIEGRSVFDLTGRELSRRVGSV
                     FQDPEAQLVSAGVEEELAFGPENLGFEPDEIERRIDFALQAVRIPHLRKASIAGLSGG
                     QKQRVAIAAVLAMRPDILILDEPTAELDPLGTDDVFAVLRQLNVEYGITVIIVEHKME
                     HLAEYCRRLLVMEEGRIRIDGTPADVFSDWFGIKELGVRLPQVTEFFGNLPEEFRSGT
                     RVPVTLEQTTAIIRRFFINPEKDGERQ"
     gene            1915..>2314
                     /locus_tag="VF531_15375"
     CDS             1915..>2314
                     /locus_tag="VF531_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715397.1"
                     /translation="MTAIIQIQDLVQNYGGKAPVLDHIRLDIGRGEFVALVGQNGAGK
                     STLVKHFNGLLRPDSGRVLVCGRDTQRVTTACLAQQVGYVFQNPDHQIFLERVDKEVA
                     FGPGNLGLNAAEVECRTVKALEQTGLTRFRE"
BASE COUNT          536 a          515 c          670 g          593 t
ORIGIN      
        1 tagaaaattg gttgttcgat tgactttcat aaacgatttt gctatattta agataagcat
       61 tatagagcag agtttaggta gtttgaggca gcaatagcat agctgagttc atgcagtgga
      121 tggccgctgt gccataaacc gcatgggaga cgagtgcagg gttgaaaacg ttgcaatcat
      181 tgttacgttc ataacgccag gcgcgagttg cctggctttt ttatattctt tttcgatgag
      241 ggtatttggg aggagcggct gttatccggc aagttacata ccagtacacg gagcattcct
      301 aaaaggcgca tacaaatggc cgaatcgggt agtcgctaaa ccggtatgat gtttttacgc
      361 gtacttacaa gttacttgcc ggattcaaga taaaaaattc agcataggag aggattggtg
      421 ttcagatgag tggcaatgta gagacgagag tagttaaaca tcagggctta caggcgatcg
      481 atctggcggt ggcggcgtta ctgattgcag cgggagcggt aattcggttg acggttccac
      541 cgatgttcgg gattacgccc aatttcgcca tcgccatgta ttcgctggcg attatccttg
      601 cccggccgag gttaggggag gccatcggca tcggcgtggt tgccggactg gtttgcatgt
      661 tcacctccaa ggcggcgtat ccggtcaatc tgatcagtga gccgattggg gctatggttt
      721 gcctgggcat cttcagttta ctggaggcta aatttaaggg atttgtctta actccgctgc
      781 ttaccggggc actggctacc ctggcgagcg gtttgagttt tgtagcggtt tatgtagtct
      841 ttaaactggt accgctggcg gtggcagcca acatgctcat cacagtggtt actttaacgg
      901 tattaaatgc aatcattgtt caagtgcttt attttcccgc caagaagctt ttgaagaaat
      961 aatacgttga ttgaaccgag aaaaaatctc ttcaaggagg ccgactgtga ctttatttca
     1021 gcaagagcct cttgcaaaaa aggaagagcc gctcatcgcg gcccggaatc tcagtttcag
     1081 ttatcatgat tcccccaccc cggcattgcg ggagatcaac ctgacagtca gcaagggtga
     1141 gtttatcggg atcaccgggc cgaccggagc cggaaagacc acccttgctc attgtttaaa
     1201 cggggtgatt cctcattttg tcaccgggga ttattttggt gacgttgcga ttgaaggccg
     1261 ctcggtgttt gatctgaccg gacgggaact ctcccgccgg gtaggttcgg tctttcagga
     1321 tcccgaagcc caactggtct ctgccggtgt tgaggaggaa ttggcctttg gcccggagaa
     1381 tctggggttc gagccggatg agatcgaacg acggattgac tttgccttgc aggcggtcag
     1441 aattccccat ttacgaaagg cctctattgc aggtctctcc ggcgggcaaa aacaacgggt
     1501 ggccatcgcg gcggtactgg cgatgcggcc cgatatcctg atcctggatg aacccaccgc
     1561 ggaattggat ccattgggta ccgacgacgt cttcgcggta ctgaggcagt taaatgtgga
     1621 atacgggatt acggtaatca tcgtagagca taaaatggaa catttggccg agtattgtcg
     1681 aagattgctg gttatggagg agggccggat ccggatcgac ggaactccgg ccgatgtttt
     1741 cagcgattgg tttggtataa aggagttggg cgttcgcctg ccccaagtca ccgagttttt
     1801 cggtaacctg ccggaagaat tccgtagtgg aactcgagtt ccggtcacct tggagcaaac
     1861 aaccgctatc atccggcggt tcttcatcaa tcctgagaaa gacggggaac ggcaatgact
     1921 gcaatcattc aaattcagga cttggtacag aattacggtg gaaaggctcc ggtgcttgac
     1981 catattcgtt tggatatcgg caggggcgaa tttgtggctt tggtggggca aaacggagcg
     2041 ggtaaatcca cactggtcaa acattttaat ggattactcc gtcccgattc cggccgggtt
     2101 ttggtctgcg gccgtgacac ccagagggta accacggctt gtctcgccca gcaagtaggc
     2161 tatgtatttc aaaacccgga tcaccagatt tttctggaac gggttgacaa agaggtagcc
     2221 tttggtccgg gtaacctggg acttaatgcc gccgaggtcg aatgccggac cgtgaaggct
     2281 ttggaacaaa ccgggttaac gcgcttccgg gagg
//
LOCUS       DASVMB010000548         7034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_143716,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000548 DASVMB010000000
VERSION     DASVMB010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143716"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF531_15380"
     CDS             <1..260
                     /locus_tag="VF531_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HEX7715398.1"
                     /translation="EKMAVEAGYWPLYRYNPELALEGKNPFMLDSKEPTGSYRDFIMG
                     EVRYATLKKQFPEQAEDLYNRAEQDAKARLEMYKKLAAVNS"
     gene            complement(391..1947)
                     /locus_tag="VF531_15385"
     CDS             complement(391..1947)
                     /locus_tag="VF531_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7715399.1"
                     /translation="MSILTAENISHGFGARKILENASFRLLKGEHVALVGANGEGKTT
                     FLKIITGQLTPDEGKIEWCKRITTGYLDQFTVLTKGKTIREILREAFQPMFDLEKEML
                     EAYEKMAVASEAEINAMMEDVGEIQSTLEHNGFYSIDAKIEEVANGLGLGEIGLDREV
                     SDLSGGQRAKVLLTKLLLQNPMILIMDEPTNFLDAHHIQWLTNYLKNYENAFILVSHD
                     IPFLAAVANVIYHVENASLTRYTGNYHDFEEMYQLKKEQNQKAFERQQKEIERLEDFV
                     ARNKARAATANLAKSRQKKLDKMELIEKDRERLKPVFKFKEARTPGKYIFQANNLVLG
                     YDEPLTRPLNLILERGQRVAIKGVNGLGKSTLLKTLLGVIQPVAGTVKLDDYLIPGYF
                     EQESAGSETNTALEEIWNEYPGLSNAEARGALAGCGLTREHISTQMMVLSGGENAKVR
                     LCKLMLKEINWLVLDEPTNHLDVDAKQALEKALREFKGTILLVSHEPEFYRDWVTDIW
                     NLEDWTTKIS"
     gene            complement(2057..2764)
                     /gene="deoD"
                     /locus_tag="VF531_15390"
     CDS             complement(2057..2764)
                     /gene="deoD"
                     /locus_tag="VF531_15390"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX7715400.1"
                     /translation="MIPTPHIQVDKDGVISETVLLPGDPLRAKFIAEHYLENPVQFNA
                     VRNMYGFTGTYHGKKVSVMGTGMGMPSIGIYSYELIHFYGVKNLIRVGSCGALQPEVK
                     VRDIVIGMAASTDSNYAAQYHLPGTFAPTASWKLLKKAVTAADQKAISVKVGNIVSSD
                     VFYGDDSEAWRKWAMMGVLAVEMEAAALYMNAARAGVNALCILTVSDSLVSHEETTAA
                     EREQSFTQMMELALELA"
     gene            complement(2859..3743)
                     /locus_tag="VF531_15395"
     CDS             complement(2859..3743)
                     /locus_tag="VF531_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7715401.1"
                     /translation="MTGEANHKIAISGDLGSGKSTVCRLLKERLSFGSYSMGETWRRL
                     AEKYQMTILELNRYSETHPLDEEMDRDLTETGQAPGNMIFDSRLAWHFVPDSFKVHLV
                     VDSRIAASRIYNDLRGQAEGYASVEQAHALLQERKASENRRYLQKYGVDCTRLQNYDL
                     VVDTSMASPERIAELICTCFEAWRNGKTFTPLWLSPQSLFPARESDGSGCDRSEVALL
                     EVDCFLYIWEGLETVSHALREGSPWVAARVIARDEEFPATRNDSARKLVQKHCRLELL
                     QDWEAQHQFRFSKYPSIF"
     gene            complement(3965..4141)
                     /locus_tag="VF531_15400"
     CDS             complement(3965..4141)
                     /locus_tag="VF531_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715402.1"
                     /translation="MNDVMVAKIIMAINELGSARTLLTAALKEGGDEAVKIVAVLDKI
                     KVCETDLQNIQAGK"
     gene            4394..5317
                     /locus_tag="VF531_15405"
     CDS             4394..5317
                     /locus_tag="VF531_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7715403.1"
                     /translation="MATIRPLVFSDIPVIQQMDQRSFHRDNWRSPENIANLYAADPEG
                     CYICQADEKPVGYIFCRTFGSIGYFGPLGVDPDEQNKGYGKQLIGVGLDYLRKHCRVI
                     GLETMPEWGKNIGLYHSMGFRSLQPARIMEKKLSSDLRPTKSFIVLRSGAELGARDLS
                     RVIQAVGEWTVRIYPGLDINKDLLLFLGKYPQQIVFVLIGNHPAGFIAFHPDFNCYPW
                     GMVEPGPDDSEVFRGLLRGLEGIYRSAIPMPTPTTAPTLRFHTRFRRLTGLLLDEGYR
                     LVRDTELMVLEDYQDRYPSFSDMLLLRAWIG"
     gene            5400..6677
                     /locus_tag="VF531_15410"
     CDS             5400..6677
                     /locus_tag="VF531_15410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715404.1"
                     /translation="MSLRIRPFANSLGSLQGNTRTAVLFEPLWGIPYTLYNFYLSLYM
                     KSQGVTDEQIGFLISLGYVAGAFWAFFSGKITDALGRRKTTIIFDLIAWPGSILIYIF
                     ARSFPMFALATLVGSAVRVVSVSWNLMVVEDADNEQQVLAYNAMNIINISVGILTPVA
                     GIFVLYLGIVNAERILMGFAVVSMTLMMLGRNYYFRETRVGQQILDERRAHRGKFRPR
                     TNSFRELTRYLKNRPQVGKALLITVLFNVYLPIGTYTSLYFAPYLTEGLRLDKSAISL
                     LGGVNAAVMLLVLILLIPRIPRINRSNWNTCMRTGIVIQAIGLVFLIISPPGRFLPAL
                     GGISIIAMGFAIGKPFLDTLLANVTGGKERSGIFALNNTVISVLCAIIGLASGYLYNI
                     RPYLIYIFSLGLLALIFMVLLRLEPGNPPEDQP"
     gene            6863..>7034
                     /locus_tag="VF531_15415"
     CDS             6863..>7034
                     /locus_tag="VF531_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7715405.1"
                     /translation="MLDIKLVRNDPAKVREAMAKRGANVPLDQFLALDEERRKKLVEV
                     EQLKNRRNVVSKE"
BASE COUNT         1647 a         1720 c         1769 g         1898 t
ORIGIN      
        1 aagagaaaat ggcggttgaa gccgggtact ggccgttata ccgctacaat ccggagctgg
       61 ccctcgaggg taagaatccg tttatgctgg attccaaaga acccaccggc agttaccgtg
      121 acttcatcat gggcgaggtt cgttacgcca ccttgaagaa gcagttcccc gagcaggcag
      181 aggatctcta caaccgggcc gagcaagatg ccaaggcaag gctggagatg tacaaaaaac
      241 tggctgcggt aaacagctaa gagagttcag ttaacaagaa gtattgagtt aaaacaaaac
      301 caggccgata ttctaatcgg cctggttttg ttaaatgccc attcgttgat caaccaatac
      361 ccggtgtaac gtgcccaatc aaaaaacatg ttaagaaatc ttcgtcgtcc aatcctccaa
      421 gttccagata tcggtcaccc aatcccgata aaactcgggt tcgtggctga ctaggagaat
      481 ggtgccttta aattcccgta aagctttctc cagagcctgt ttggcgtcga catccaagtg
      541 gttggtgggt tcatccaaaa ccagccagtt gatctccttc agcatcaatt tgcacagtcg
      601 caccttggcg ttctctccac cactcaacac catcatctga gtggagatat gctcccgagt
      661 cagtccgcag ccggctagcg cccctcgagc ctcggcgtta cttaaccccg gatattcatt
      721 ccagatctcc tcaagggcgg tgttcgtctc cgaaccggcc gattcctgtt caaaataacc
      781 gggaatcagg taatcatcaa gtttaacggt tcccgccact ggctgaatga cacccagtaa
      841 agtttttagc agggtggatt tgccaaggcc attgacgcct ttgatggcta cccgttgacc
      901 ccgctccagt atcagattta aaggccgggt caggggctca tcgtaaccaa ggaccagatt
      961 gttcgcctga aagatgtact taccgggagt ccgggcttct ttgaatttaa ataccggctt
     1021 caaccgttcc cgatcttttt cgatcagctc catcttgtcc agttttttct ggcgactttt
     1081 ggccagatta gccgtagcgg cccgggcttt gttccgggct acaaaatcct ccaatcgttc
     1141 gatctccttt tgttgccgtt cgaaagcctt ttgattttgc tctttcttta attggtacat
     1201 ttcctcaaaa tcatggtaat taccggtgta ccgggtaaga gaggcgtttt ccacatgata
     1261 aatcacattg gccactgcgg ctaagaacgg gatgtcgtgg gataccagga taaaagcatt
     1321 ctcatagttt ttcagataat tggtcaacca ttggatatga tgggcatcta agaagttcgt
     1381 gggttcgtcc atgatcaata tcatcggatt ctgcagcaat agtttggtta gcagtacctt
     1441 ggcccgttgc ccgccgctta aatcggaaac ctcccggtcg aggccgattt caccgagccc
     1501 taaaccgttg gcgacttcct cgattttggc gtcgatggag taaaatccgt tatgttccag
     1561 ggtgctctgg atctcgccta cgtcttccat catcgcgttg atctccgcct ctgaagcgac
     1621 ggccattttt tcataggctt cgagcatctc tttttccagg tcgaacatgg gctgaaaggc
     1681 ctcccgtaaa atttcccgga tggtcttgcc tttcgtcaat acggtgaatt gatcaaggta
     1741 tccggtggta atccgtttgc accattcgat cttcccttcg tcgggtgtca gttgaccggt
     1801 gatgatcttg agaaaggtag tctttccttc gccgttggcc ccgaccagcg ccacatgctc
     1861 acccttgagg agccggaaag atgcattctc cagtattttt cgggcgccaa aaccgtgact
     1921 gatgttttca gctgttaaaa tactcatttt tcctcacgct atcgtagatt taggtttgaa
     1981 gaacgaaaaa attcaatcca cccgaaatta cacgatggat tgaataggga cttgatatga
     2041 aaaatccccg gtagaatcaa gccagctcta atgcgagctc catcatttga gtgaaagact
     2101 gttccctctc ggccgcggtc gtttcctcat gagagactaa cgaatcggag acggtcagga
     2161 tacagagcgc attgactccc gcccgggcgg cattcatgta aagggccgcc gcttccatct
     2221 ccacggccaa cactcccatc atggcccatt tacgccaagc ctcggaatcg tcgccgtaaa
     2281 aaacatccga tgagacaata ttgccaacct tgacggaaat agctttttga tccgcggcgg
     2341 ttacagcctt ttttaataac ttccaggatg cggtgggggc aaaggttccg ggcagatggt
     2401 attgggcggc ataattcgag tcggtggagg ccgccattcc aatcacgata tcccttacct
     2461 tgacttctgg ttgcagcgcg ccgcaggaac cgacgcggat caggtttttt acaccataga
     2521 aatgaatcaa ttcgtaggag tagataccga tggacggcat acccatccct gttcccatca
     2581 ccgagacctt ttttccgtga taggttcctg tgaatccata catgttccga acggcattaa
     2641 attggaccgg gttctccagg taatgttcgg cgatgaattt ggcgcgtaag ggatcgccgg
     2701 gtaataatac tgtttctgaa atgactccat ccttgtcgac ttgaatatgg ggcgtcggta
     2761 tcatctgatc actcctataa tatagttgat tttcaaattg aacttaggtt aaattggttt
     2821 ccactcaaag gaattaagtt accgtgtttt aagaagcatc aaaaaatgga ggggtatttg
     2881 ctgaaccgga actgatgctg ggcctcccag tcctgaagca attccagccg gcagtgtttt
     2941 tgcaccagtt tccgggcgga atcgttccgg gtggccggaa attcttcatc ccgggcaatg
     3001 acccgcgccg caacccacgg agatccttcc cgcaaggcgt ggcttacagt ctccaggccc
     3061 tcccagatgt aaaggaaaca atccacttcg agtaaggcta cttccgaacg atcgcatccg
     3121 gagccatcac tctcacgggc gggaaatagg gattgaggtg acagccacaa tggagtaaag
     3181 gttttaccgt ttcgccaggc ttcgaaacag gtacagatca attcggcgat ccgctcggga
     3241 cttgccatag aagtatcgac caccaaatca taattttgga gccgggtgca atctactcca
     3301 tatttctgaa ggtatcgccg gttctcactg gccttccgtt cctggagtag ggcatgagct
     3361 tgttcaacac tggcataacc ttcggcctga ccgcgtagat cgttataaat gcgggacgcc
     3421 gcgattcgac tgtccacgac gagatgcacc ttgaaagagt ccggtacgaa gtgccaggcg
     3481 agcctggagt caaagatcat attgcccggt gcctgacctg tttcagtcag atcccggtcc
     3541 atctcctcat ccaggggatg ggtctcggaa taccggttta actccaggat tgtcatctgg
     3601 tatttttccg ccaagcgtcg ccaggtctca cccatggagt aactgccgaa ggagagccgt
     3661 tctttaagta atcggcaaac agtgcttttg ccgctgccca ggtcgccgga gatggcgatc
     3721 ttatgatttg cttccccagt catctataaa tctcctgttg tttaagtgtt ttccatttcg
     3781 aaactaacgg ttactctgga gatgatacga ggttgatatt gtttttatta tacccgtcat
     3841 tggaacgggg ttcaatcaaa atccggtcgg tttgaatatt tttggggttg atcttgtaga
     3901 cttgagcctg ggcgaggatc tgcctgacag cggtatcgtt ttgataaaga cctcctcctt
     3961 atattcattt acccgcctga atgttttgga ggtccgtctc gcaaaccttg atcttatcta
     4021 aaactgccac gattttaacc gcttcatcgc cgccttcctt caaagcagcc gttagaagtg
     4081 tacgagccga acccagttcg ttaatagcca tgatgatttt tgccaccatt acatcgttca
     4141 tcagatatac ctccagcttc attcctgcca aaacccgaag aggtttctgt tgatcttaat
     4201 tcggccggtg tccaacgggt tgctacaggc taataacatt atactacact tttcatttta
     4261 aagtaaaatt tatccaagga ttaatattct tcttccgcac ccggagggaa ggagtcaacc
     4321 ccggaatggc gaatagtttg gcgatatagg tgaagcgtga tatagatgaa acttcatccg
     4381 aggtgatttt tggatggcga ccatccggcc gctggtcttt tccgacattc ccgttattca
     4441 acagatggat caaaggagtt ttcatcggga caactggcgt tccccggaga atatcgccaa
     4501 tttgtacgcc gcagatcccg aagggtgtta tatctgccag gcggatgaga agcctgtggg
     4561 atacatcttt tgccgtactt ttggctccat cggttatttc ggaccgttgg gggtggatcc
     4621 cgacgagcag aacaaggggt acggcaagca attgattggg gtgggactcg attatttgcg
     4681 gaaacattgc cgggtgatcg gactggagac gatgccggaa tggggcaaga atatcggatt
     4741 atatcacagc atgggctttc ggtcgctgca accggcccgg atcatggaga aaaagctttc
     4801 gtccgatctg cgacccacca aatcttttat tgtcttacgt tccggagcgg aactcggcgc
     4861 cagggatctc tcccgggtga tccaagctgt tggagaatgg acggtccgga tttatccggg
     4921 tttggatatt aacaaggatc tgttgttgtt tttgggtaag tatccgcaac agatcgtttt
     4981 tgttctgatt gggaaccatc cggcaggttt tattgccttt catcctgatt ttaactgtta
     5041 tccatgggga atggtggagc cgggaccgga tgacagtgaa gtatttcggg gtttactccg
     5101 tggtttggaa ggaatttacc gttccgccat accgatgccg acgccgacaa cggcaccgac
     5161 tctcaggttt catacccgtt ttcgacgttt gaccggattg ttgctggatg aggggtatcg
     5221 tttggtgcgg gacacggaat taatggtact cgaggattat caggaccggt atccatcgtt
     5281 tagcgatatg ttgcttctcc gggcgtggat aggttaaagc aaggggtcaa acagtctatg
     5341 aatggctgtc atccccacca atcagcatga ataggatcat aacaagggag agaaggacct
     5401 tgagtctacg aattagaccg ttcgctaatt cattgggttc gttgcaaggg aatacccgga
     5461 ccgccgtact tttcgagccg ttatggggaa ttccttatac gttatacaat ttttatctta
     5521 gcctgtacat gaaaagccag ggtgtgaccg atgagcagat cggttttctc atctccctgg
     5581 gttatgttgc cggtgcattt tgggctttct ttagcggaaa gatcaccgat gccttggggc
     5641 ggagaaagac caccatcatc tttgacctga ttgcctggcc gggttcaatc ctgatctaca
     5701 tttttgccag aagttttccg atgtttgccc tggccacact ggtgggtagt gcggtccggg
     5761 tcgtttcggt ctcctggaac ctaatggtgg tggaggatgc cgataacgaa caacaggtgt
     5821 tggcgtacaa tgccatgaac atcatcaaca tttcggtggg catactcact ccggtggctg
     5881 ggattttcgt actttacctg ggaattgtca acgccgagcg aatcctgatg ggatttgcgg
     5941 ttgtcagcat gactttgatg atgttgggca gaaattacta cttccgggag acccgggtgg
     6001 ggcaacagat ccttgacgaa cgccgggcac atcggggaaa attccggccc cggaccaatt
     6061 cattccggga attgacgcgg tatctcaaaa atcggcccca agtcggcaag gccttgttga
     6121 ttacggttct gttcaatgtg tatctcccta tcggtacata cactagtcta tattttgcgc
     6181 cttatcttac cgaaggtctc cggcttgata aatccgccat atcgctcttg ggcggagtca
     6241 acgccgctgt tatgcttttg gtgttgattc tgctcatccc gcgaattccg cggatcaacc
     6301 ggagtaactg gaacacttgc atgcggaccg gcatcgtgat tcaagcgatc ggcctggtgt
     6361 ttctgatcat ctctccaccg gggcgctttc taccggcgct tgggggaatc agcatcatcg
     6421 ccatgggttt cgccatcggg aagccgttcc ttgatacact gctggccaat gtcaccgggg
     6481 gaaaagagcg atcgggtatc tttgcgctga acaacacggt catatccgtt ttatgcgcaa
     6541 tcatcggctt ggcctcgggt tatctctata atatcaggcc ttatctgata tatatctttt
     6601 ccttaggttt attggcattg atatttatgg tattactcag gttggaaccg gggaatccac
     6661 cggaggatca accttaattt acgtttgacc ccgcccggtt cggatgattg gtcggtccga
     6721 acggtccggg ttatccggaa tccaaggtaa acataagcgg ttggattgtt ttttaataat
     6781 gattgggtta taatgggtaa cgtaattcta cgttaccttt ttacttacac ttacctatat
     6841 cgaaattgaa ggagtgtccg ggatgcttga tattaaactc gtccggaacg acccggccaa
     6901 ggtccgcgag gcaatggcca agagaggcgc caatgtcccg ctcgatcaat tcctggcctt
     6961 ggatgaggaa cgccgcaaga aactggtcga ggtagaacag cttaaaaacc gccgtaatgt
     7021 ggtatccaag gaag
//
LOCUS       DASVMB010000549         7926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_143859,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000549 DASVMB010000000
VERSION     DASVMB010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143859"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VF531_15420"
     CDS             <1..623
                     /locus_tag="VF531_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715406.1"
                     /translation="APASVNVVTGLQSNDSDAFISASGEIVFMRDVNQSSSAIWTMSS
                     DGNIQKFADAAGYYCPTISSDGTKVVTQYYSDHKPRGLYLFTPGTTNPQLLVELTTSE
                     AGYSVISPDGTKVAYVADDGNVYTMNTDGNGTPLKLTNWHYGTTREPAWSSDGTKIAY
                     VLHFSLSVDDKFGVYIMNSDGTQNYLFREVQTNDPSASRFKYPVLR"
     gene            1158..2219
                     /locus_tag="VF531_15425"
     CDS             1158..2219
                     /locus_tag="VF531_15425"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX7715407.1"
                     /translation="MNGAVTSEYRCWQFTANTPNTTRFGGTDSYQTGVAVAEAVWNNP
                     ADPMERPGVVILARGDGSCFQEALIASSLIHFPRNGPVLLTEPDFLNPVVRSEIIRLC
                     PSGKGSPAQVLTIGSLSPRIDEEVQRMGFTVCRINGGDPATTAVIIWNLLGPKCNVML
                     VSEEQFGDALPAGGWAAHMGDPILLTHGQQLPGVTAQAIRERQPDVYIIGGPGSISPR
                     VEETVRHLTRGFVDRIAGPDPAATSVRFTRYQSPTGTFGWNVNQKKGWSFRFSRIDSW
                     QPAVSGNPLSHMGKHSPLLLVHPQMVPSPVAEFIGAVNPLHREPEPPFMHGFIIGNCL
                     EVSCQVQIELDKRLETVKE"
     gene            2387..2818
                     /locus_tag="VF531_15430"
     CDS             2387..2818
                     /locus_tag="VF531_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7715408.1"
                     /translation="MKVSDLLKEKGGQVITVSPEASIFEAMRLLVENKIGSLVVRDDE
                     GKLVGILSERDILKAAFEGYDSLKAKKVTNLMTTNLIVAIPEDDLEYIKGIMTVNHIR
                     HLPIVTRDGIVGIVSIGDIVKYQLQETQVQNRYLEEYMFGQ"
     gene            2883..3614
                     /locus_tag="VF531_15435"
     CDS             2883..3614
                     /locus_tag="VF531_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7715409.1"
                     /translation="MEKPGSGGKLEILSRVNLTIHRKRITALIGPSGAGKSSLLRLLN
                     RLDDPSDGSVFLNARPLTSYDIPELRRRVGMVFQLPVIIPGTVAENLHLAARFAPKGR
                     KLELIPVPELLTTVGLDPEFAGREAGRLSVGQQQRVSLARTLAVRPQAVLLDEPTASL
                     DPESANAVMDLVRRLRDGLGLTVVLVTHLLAQARQYADYTAYLEGGRLIEFNETGALF
                     DSPREIRTRQFLNGGGQERELYPNH"
     gene            3592..4374
                     /gene="fetB"
                     /locus_tag="VF531_15440"
     CDS             3592..4374
                     /gene="fetB"
                     /locus_tag="VF531_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX7715410.1"
                     /translation="MNYIPITDLDLLVAVSLIGVTLGISIRERLGLERDLLIGAVRGF
                     IQLMLVGYVLQYIFDLRRWYLVILILAIMILVAGSNALKRQKLRWRRLFWILTAAIAG
                     GSVLVLAVVIGFILKVRPWYLPQYMIPLAGMIIGNSMVGAALAANRLSADIKDGRPRI
                     ELALALGATSRQAVAPYLREALKAAMLPTISSMMTVGIVQLPGMMTGQIISGTNPGDA
                     VRYQIVVMYMVAAATALTVIVVVLGVYRSFFNSYLQLLDEPR"
     gene            complement(4587..5897)
                     /locus_tag="VF531_15445"
     CDS             complement(4587..5897)
                     /locus_tag="VF531_15445"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX7715411.1"
                     /translation="MPELPWDSGSLELPLLRGRAINPSLAWLEPTLPAPLSKIELQTQ
                     LDKEFQLIAPQIKKAARIALLLEDPSRPSRTRELAASIIRKIGETRGGLTGVSVIVAA
                     GAHYRIEPEALAMKLPALDPPAVIHDATSTSNLAHLGFSQSGVDFWFNRNIAEAELRL
                     SISTVNLHPLVGFSGGGKILLPGVAGIETIRGLHRLPAGTAGAAQTAMRDLINEVLDR
                     LPIAYSWHLLSRPDGGIFRIFAGPVQSSHSEARNELLRLVTLEPPSVQPQLLILGAHP
                     FHQNLIGSFKSLHQIPAVLAPGGCALLINEATQGVGFHHWRNDPAVTAAEKEKCHRMY
                     QAYRVALYSPGTQPEAFRNLFPEEFVLLRSFQELTDFLKEFADQPAAVIPYAPVTLIR
                     QQGTGNRHEARGVRREGLGDRQEGIGQAGVLRDICVESLFTLSH"
     gene            complement(6087..6920)
                     /locus_tag="VF531_15450"
     CDS             complement(6087..6920)
                     /locus_tag="VF531_15450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7715412.1"
                     /translation="MKKPTAFWMTLAIVCVFAISFVFSHTAGASSGKPAVAAQAAILI
                     NAVNGEILFAKKADRVMAPASTTKIMTAILGLERGDLGRTVTVSTNAAAKDGSSMHLA
                     CNQRITLRNLLYGVLLISGNDAATAVAEYIAGSETAFARLMNAKAIGLGMRNTRFQNA
                     SGLPAVNHYSTAYDLALLTRYAMRNPNFAWIVQTKTYSMQGNRPGERVQLINHNKLLW
                     RYPYCTGVKTGYTIRAGGCLVSSAAKQGKQLIAVVLHSSAIYDDSQKLFEYGFKRLSP
                     R"
     gene            7323..>7926
                     /locus_tag="VF531_15455"
     CDS             7323..>7926
                     /locus_tag="VF531_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715413.1"
                     /translation="MIQILIVEDEANISNLLKTHLEREGYSCIQAFNGIEALERYNAI
                     HPVLVILDLMLPGIDGLEVCRTIRRQQYDTYILMLTAKQEEVDRVLGLEIGADDYMIK
                     PFSIRELLARVKVLLRRPHLSGEGDSDQSVVIRGDLELDPARMSVKSQGHPVSLTALE
                     FDLLYFLMKNAGLVFKRDQLLEKVWGEDPAGRGEVFDRNID"
BASE COUNT         1841 a         1941 c         2174 g         1970 t
ORIGIN      
        1 ccgctcctgc atcggtaaac gtagttacag gattacaatc taatgactcg gatgctttca
       61 tctccgccag tggtgaaatc gtgtttatgc gtgacgtaaa tcaatcgtct tccgctattt
      121 ggaccatgag ttccgatggg aacattcaaa agttcgccga tgcagcagga tactattgcc
      181 caactatttc aagtgatggt accaaggttg ttacacagta ttactcagat cataaaccaa
      241 gaggccttta cctgtttacc ccgggaacaa ccaacccgca gctccttgta gaacttacta
      301 catctgaagc cggttactct gttatttctc cggatggaac caaagttgct tatgtggcag
      361 atgatggaaa tgtttatacg atgaatacgg atggcaacgg aactccgttg aaactcacca
      421 attggcatta cgggaccacc cgagagccgg cttggtcctc cgatggtaca aagattgctt
      481 atgttttgca tttttcatta tcagtggatg ataaattcgg cgtttatatc atgaatagcg
      541 atggaaccca gaattatctc tttcgagagg tacaaaccaa tgatcctagc gcgagtagat
      601 ttaaatatcc ggtgctgcgt taatagaaaa gattctcacg gaatttaaat ccatagtatt
      661 tgcaaaagaa attcggagtg ccctgactgg actccgaatt tctttatatc aatacgaatt
      721 tctcgtggcc cccgaaaatg ttttttgatc tgggctataa atcgagtcta gttagtattt
      781 ggctcctaag accttcattt tgcgctacat tatttcctgc aaagcgttgg ccatttttct
      841 tgcaaactct tccggttttt gatcatcata tgtgaatgga taaagttttt gccaatcatt
      901 tcgcattttt tatttgaaaa aagcatttgg aacagaacca atatcaattt tttactttgt
      961 cacttcgggt taaattttgg cctgttattt tttcgttggt ttctctaaaa tcccgcttga
     1021 ttggttttca tgaatataaa gctgtgtttt ttcgattcat caggaatgat caacccctat
     1081 cccggcggga caatgttcaa aaaccccgct gacggcatag aaaatgataa agaaagaatg
     1141 ggaaacgggg tgtccaaatg aatggtgcag tgacgtcaga atatcggtgt tggcagttta
     1201 cagccaatac tcccaatact acccgttttg gcggaaccga ttcctatcag accggggtgg
     1261 cggtagcgga ggcagtttgg aacaacccgg ccgatccgat ggaacggccg ggtgtggtta
     1321 ttttagcccg gggtgacggg tcatgttttc aggaagccct gattgcgtct tcactgatcc
     1381 atttcccgcg gaacggaccg gtgcttttga cggaaccgga ctttttaaat ccggtggtgc
     1441 gaagtgagat tatccggctc tgtccgtcgg gaaaaggatc cccggctcag gttttaacca
     1501 tcggctcctt atcaccgcgg atcgatgagg aagttcagcg aatggggttt acggtctgcc
     1561 ggattaacgg aggagatccg gctacgacag cggtcatcat ctggaatttg cttgggccga
     1621 aatgtaacgt gatgctggtc tcggaggaac aatttgggga cgcgttgcca gccgggggat
     1681 gggcagcgca tatgggagac ccgattttac ttacccatgg ccagcaattg ccgggagtta
     1741 ccgctcaggc gattcgcgag cggcaacccg atgtttacat tatcggaggt ccggggagta
     1801 tttcaccccg cgtggaagag acggtgcgcc acttaacccg gggatttgtg gatcgaatcg
     1861 ccgggcccga tccggcggct acctccgttc gttttacacg gtatcagagt cccaccggaa
     1921 cattcggatg gaatgtcaat caaaaaaagg ggtggagttt ccggttttcc cggatcgatt
     1981 cctggcagcc ggccgttagc ggaaatccct tgtcccatat ggggaaacac tctccgctac
     2041 tgctggtcca cccgcagatg gtgccgtcgc cggtggcgga gtttatcgga gcggtcaatc
     2101 cgttacaccg ggaaccggaa cccccgttta tgcacggatt tatcattggc aattgcctgg
     2161 aggtttcctg ccaggttcag atcgaactcg ataaacggct cgaaaccgta aaagaatgag
     2221 agaaccctta caggattttt ccaaccggtg gcaaatagta taaatgatgc cagcggttgt
     2281 ttattttagg acgcgattgg ttatgctaaa agtccggtat ttccacttcc aatgctcccg
     2341 aaaccatcga tctgccaaac gaaaaaagac agaggagagt gagggcatga aggtatccga
     2401 tttgttaaag gaaaaaggcg gtcaggtaat caccgtctcc ccggaggctt cgatctttga
     2461 ggccatgcga ctgttggtgg aaaataaaat cggctctctg gtggtccgcg acgatgaagg
     2521 caagctggtg gggatcctct cggaacggga cattttaaag gcagcttttg agggttacga
     2581 ctcattaaaa gcgaaaaagg tcaccaatct gatgaccacc aacctgatcg tggccattcc
     2641 cgaagatgat ctggaatata ttaagggaat catgaccgtc aatcacattc gtcatctgcc
     2701 cattgtgacc cgggatggaa tcgtgggaat cgtctccatc ggggacattg tcaaatacca
     2761 gttacaggag acgcaggttc agaaccgtta cttggaggag tacatgttcg gacagtagga
     2821 agagttccgt atcgacgcta aaaaagggtt gatctgccat ctttcaactt catcaggtat
     2881 cggtggagaa accgggtagc ggaggaaagc ttgagatcct gtcccgggtc aacctgacaa
     2941 ttcacaggaa acggattacc gcgctcatcg gtccttccgg ggccgggaaa agtagtctgc
     3001 tccggctgct caaccgcctg gatgatccca gcgacggctc ggttttcctc aacgctcgac
     3061 cgctcacatc ctatgatatc ccggaactcc gccgccgggt cggaatggtc ttccagctgc
     3121 cggtgattat tccgggaacc gtcgcggaaa atctccatct ggcagccagg ttcgctccca
     3181 aaggacgaaa gttggaactc atacctgtcc cggagctttt aacgacagtc ggtctcgacc
     3241 cggagtttgc cggacgggaa gcaggacggc tttccgtggg gcaacagcag cgggtctcgc
     3301 tcgcccggac tcttgccgtc cggcctcagg cagtgctgct tgatgaacct accgcctcac
     3361 tcgatccgga atctgcaaat gcggtgatgg atttagttcg gaggctccgg gacggactgg
     3421 gattgaccgt cgttttggtc acgcatctgc tggctcaggc aaggcagtat gcggattaca
     3481 ccgcatatct tgaggggggc cggttgatcg aattcaacga gaccggggcg ctctttgatt
     3541 caccgcggga gatccggacc cgtcagtttc taaatggcgg agggcaggaa cgtgaattat
     3601 atcccaatca ctgatctgga cctgctcgtt gccgtcagcc taatcggcgt aacgctcggt
     3661 atctccattc gggaacggtt gggcctggaa agagatttac tcatcggagc ggtacggggt
     3721 tttatccaac tgatgttggt cggttatgtt ttacaataca tctttgatct gcgccgctgg
     3781 tatctggtga tcctgatcct ggcgatcatg atcctggtgg ccggatccaa tgccttgaaa
     3841 cgccagaagc tccgttggag gagactgttc tggattttga cggccgccat cgccgggggc
     3901 agcgtactcg ttctggctgt tgtaatcgga tttatattga aggtccggcc ctggtacctg
     3961 ccacaatata tgattccctt ggccggaatg atcatcggca attccatggt gggtgcggcc
     4021 ttggccgcca accggctgag tgcggatatc aaggacgggc gtccccggat cgagctggcc
     4081 ctggctttgg gggcaacctc ccggcaggcg gtcgctcctt acctgcggga agccttaaag
     4141 gctgcgatgc tacccacgat cagttcgatg atgaccgtgg ggatcgtgca gcttccgggg
     4201 atgatgaccg gccagatcat ctccggaacg aatcccggcg acgcggtacg ttatcagatt
     4261 gtggtgatgt atatggtcgc cgcggccaca gcgctaacgg tgatcgttgt ggttttaggg
     4321 gtttaccgtt ctttcttcaa ctcctactta caactgctgg atgaaccgag atgaggcgcg
     4381 tggcaatagg gaacaggcaa taggcaacag ggtaggaagc ccaaatccag gtctatctac
     4441 cctatccctt taaggggatc agcttgactc ccgggctttt gtcggttagg cttcgtagtt
     4501 tagctccagc ctgttgaccc aagaatccag ccagttccct gaaaacggtg acaccgtttt
     4561 cttaagagtt gtaatacaac tccactttaa tgggaaagag tgaataggct ttcgacacaa
     4621 atatctctca atactcctgc ctggccaatt ccctcttgcc tgtcccctag tccctcacgc
     4681 ctcacgcctc gtgcctcatg cctgttccct gtcccctgtt gcctgattaa agtaaccgga
     4741 gcatacggga tcacggctgc cggttgatcc gcaaattctt tgagaaaatc cgtcaattcc
     4801 tggaatgagc gaagtaatac gaattcttcc ggaaaaaggt tccggaacgc ctccggttgg
     4861 gtgccggggg agtaaagcgc cacccggtat gcttgataca tgcggtggca tttttctttc
     4921 tcggccgcgg tcactgccgg gtcattacgc caatggtgaa accccactcc ctgggtagcc
     4981 tcgtttatga gaagggcaca accgcctggg gcaagtacag ccggtatctg atgcaacgac
     5041 ttaaagctgc cgattaaatt ctgatgaaaa ggatgcgctc ccaaaatgag cagctgtggt
     5101 tgaacggatg gcggttccag ggtgacaagc cggagcagtt cattccgagc ctcgctatgg
     5161 cttgactgga cgggtccggc aaaaattctg aaaatgccgc catcgggccg ggagagcaaa
     5221 tgccaactat aagcaatggg aagacggtcc agtacttcgt tgataaggtc acgcatcgcg
     5281 gtttgcgcgg cgccggcagt tcctgcaggc agccggtgca gtccccggat ggtctcgatt
     5341 ccggccaccc ccggaagcag aatcttgccg cccccggaga aaccaaccag cggatgaaga
     5401 ttgaccgtgg agatgctcaa cctgagttcg gcctcggcta tattgcggtt gaaccaaaag
     5461 tccactccgg attggctgaa ccccaagtgg gccaagttgg aagtagaagt ggcgtcatgg
     5521 atcacggccg gcggatccag cgcaggaagt ttcatggcga gtgcctcggg ttcgatccgg
     5581 taatgggctc cggctgcaac gatgacggat acccccgtaa gccccccgcg tgtctccccg
     5641 atcttccgga tgatgcttgc ggcgagttcc cgggtgcggc tgggccgtga cggatcctcc
     5701 aacaataatg cgatccgggc ggcttttttg atttgcgggg cgatgagttg gaactcctta
     5761 tccagctgag tctggagttc gattttactc agtggtgcgg gcagtgtcgg ttcaagccag
     5821 gccagcgacg gattaatcgc gcgcccgcgt agtaatggca gctctagact tccggagtcc
     5881 caggggagtt ccggcatgag gttactcctt ttcgacggac gaatttcctt caatcaacag
     5941 gaggtgcgta tcggtagaaa aagaacctcc atacgggggt tctcttaaaa aaatgcatgt
     6001 ttattatgta ccgctcccca aggtccaatc cgcctagaaa aacttggtca tcccgggctt
     6061 tttgtcacca gatgcggaac cgcggtttat cgcggggaaa gacgtttgaa tccgtattca
     6121 aagagttttt gactgtcatc gtaaatggcg gagctatgga ggaccactgc aatcaattgt
     6181 ttgccctgtt ttgccgcaga ggaaaccaga caaccgccgg cccggatggt atagccggtc
     6241 ttgacgccgg tgcagtacgg gtagcgccaa agcaatttgt tatggttgat gagctgcacc
     6301 cgttccccgg gacggtttcc ctgcatggag taggttttgg tctgaacgat ccaggcgaag
     6361 ttcgggttac gcatggcata gcgggttaag agcgccagat cataggcggt tgagtaatga
     6421 ttcacagccg gtaaaccgct ggcgttttga aaccgggtat tacgcattcc caaaccaatc
     6481 gctttggcgt tcatcaaccg ggcgaaagcc gtttctgaac cggcaatata ctcggcgacg
     6541 gcggtggccg catcgttgcc ggatatcagt aacacaccgt atagcaagtt ccgtagcgtg
     6601 atgcgttgat tacatgccag gtgcattgac gagccgtctt tggctgcggc attggtgctg
     6661 accgtgactg tccggcctaa gtcgccccgc tcgagtccca aaatggcggt catgatcttg
     6721 gtggtgctgg ccggagccat tacccggtcg gcttttttgg caaaaagaat ctcgccgtta
     6781 acggcgttga tgagtatggc cgcctgggca gcgacggcag gcttaccgga agacgccccg
     6841 gcagtgtggg aaaacacaaa agatatcgcg aaaacgcaaa cgatcgcaag agtcatccag
     6901 aaggcagtgg gtttcttcat ggttgcagaa ctcccgttgt gaagttgcga aattccaccg
     6961 taatataacc agtatcggct gatgcggggt gtttttaaac cattccggag attattggtt
     7021 ccgaacgtta tcactacttg agttggacgg tctcatcaat cataaacagc gattaattta
     7081 caaatctgta ataattgcct cataaataca gcacatcttt ttactaaatt atacttgata
     7141 cggaatgttt cactaatcgg acggttatga ccggtcggtt tttttaggcg gaatttcgcg
     7201 cttcggcggt aatactgcgg ggggcatctc agaaaaagac gaggaaataa atccagatct
     7261 gtccagactg gaaacatttg atcatttcgg acgtttgatc agatcggatc gggggcatta
     7321 agttgattca gatcttaatc gttgaagatg aagcgaacat ttcaaatctg ctcaaaaccc
     7381 acctggaaag ggaaggttac agctgcatcc aggcttttaa cggcattgag gccttggaga
     7441 ggtataatgc gattcacccg gttttggtaa ttttggatct gatgcttccg ggcattgacg
     7501 gtctggaggt ctgccggacc atccgccgcc aacagtatga cacctatata ttgatgctca
     7561 ccgccaaaca ggaggaggtc gatcgggtac tgggccttga gatcggggct gatgattata
     7621 tgatcaaacc gttttccatt cgggaattgt tggcgcgggt caaggtactg ttgcggcggc
     7681 ctcatctttc cggggagggt gattccgatc agtccgtagt catccggggt gatctggagc
     7741 ttgatcccgc ccggatgagc gtaaaatctc aaggccatcc cgttagcctg acggccctgg
     7801 agtttgatct gctttatttc ctgatgaaaa acgcgggcct cgtttttaaa cgggatcaac
     7861 tgctagagaa ggtttggggg gaagatccgg ccggacgcgg cgaggttttt gaccgcaata
     7921 tcgacc
//
LOCUS       DASVMB010000550         5632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_507766,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000550 DASVMB010000000
VERSION     DASVMB010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507766"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF531_15460"
     CDS             <1..802
                     /locus_tag="VF531_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715414.1"
                     /translation="TKIIAKNGRAVGVQLNDGTEEFADFVISNADGRKTLLDMLEGRF
                     MSERLRGYCAEPADETNWAVHVFLGVNRDLSKEPSSLILLLDEPVTIAGHPLTSLEMQ
                     LYGFDNSMAPQGKGTIKVELVSTYSYWKELAANKKDYEAEKQRVASQVISLLEKHFHG
                     ITNQVEVVDVPTLLTWERFMGGTHGFVNAPSKKLDILGSLFKNDDMTIPGLDNFYFVG
                     MWATSAGATFLNALSGKKAIRRICQKERIGFRVNAEQLKTGNESHCNL"
     gene            1333..2082
                     /locus_tag="VF531_15465"
     CDS             1333..2082
                     /locus_tag="VF531_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7715415.1"
                     /translation="MSNEFINLTAANIASEHLCCAIADKKHQHGVDLKRTWLIDRIPE
                     GHIFRKLNEKGKVFIEYAPLEKAWVPIIGDNYIYIYCLWVSGSFKGKGYGKELLDYCI
                     ADAKRQNKSGVCIISSKVKKPFLSEKKFMQKFGFETVDTIGSEYELMALSLNGSKPNF
                     AASAKKQSIDSETLTIYYGLQCPYIPNCIEQIESYCKTNGIPVKLIKVDTLEKAKELP
                     CVFNNWAVFYKGKFETVHLLNEGFLKKMLDR"
     gene            2241..3308
                     /locus_tag="VF531_15470"
     CDS             2241..3308
                     /locus_tag="VF531_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715416.1"
                     /translation="MAEKKVLDVVKVGFYGNTGDNRSPEDFPFPACMTSLMQYLGEDY
                     PIVELESHHQKYTQRIANLHFITASGMAFGLLWSKDYCKSCMDLTQVNEHNETVKNAF
                     AWAGYEYEIMEKASDGSNREVIRERIVESIDKGLPVLAFGIVGPPECVIISGYDHKGE
                     EITGWSHFQQWEPCEKESNGMFRRAGWYDSLWKIVFIGKKIGRSINLKDVLTLGLSIM
                     EKTDSEGYVAGLAVYDEWIRYVLSNELDTIDDETLRVRHDLHHDLVGNLAEARCWGGD
                     FLLHAAEENKDENMRGVAMCFKNIHDLCWQVWGVLGSYGQQDVWKGFRSTENRRKIAK
                     LLGDIKELDQKAMTYLKLAIR"
     gene            3433..4155
                     /locus_tag="VF531_15475"
     CDS             3433..4155
                     /locus_tag="VF531_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715417.1"
                     /translation="MTQGATKILVGDDDRDIVRLIADSLADEGFQVLSAYDGVQVLNL
                     LAQSEVGLLILDIMMPEMDGLEVCRRIRNTTAAPIILLSAKGRELDKVVGLEVGADDY
                     MTKPFSINELVARVKAHLRREKRNIPQTREPKRDVERIRFDDIAIDKDTFEVWKNDEP
                     INLSTKEFQILLYLIENQNLVVSREQIYDAIWGTGGDYGDLNTVTVHIKNLRNKLDPQ
                     NHYIKTIWGIGYKFTGTSGGNT"
     gene            4262..5524
                     /locus_tag="VF531_15480"
     CDS             4262..5524
                     /locus_tag="VF531_15480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7715418.1"
                     /translation="MEHLGEDFDLFKDHLYRMNEDIGAHLKTYYPDRNRIGAYLERTA
                     VGNGVKITIYDVAGNKIAASDHRKGFGLNLELKNFTVVGENVIYVVELVYPFKVSSFT
                     ELNSFRQIRDVAVAMLAILIVLLIIYLHFSLVRPLATLNRGLESINYRNTRFQIPAVT
                     YKRRDELGELARKFGAMQQRLDASYREQSEMIASISHDLKTPLTSILGFLERLLNRKL
                     PPEQEDEYHRIIQQKARDINELVTEFNDFATADLKDDRLESMAPVDLRRFFSSICAEY
                     RSELLSRGVQFQETVRIENGVQPALRIDPQKIRRVFANLVGNSLKYGDNLSRIGFSCT
                     VRENQAIFSVEDNGPGVPESELKALFEKFYRLERSRSRDKGGSGLGLAICRSIVEHYG
                     GEIRAYLPAGSGLGVRFVLPLDGLHKSS"
BASE COUNT         1574 a         1116 c         1489 g         1453 t
ORIGIN      
        1 gaccaagatc attgcaaaaa acggccgggc agttggtgtc caattgaatg acggcaccga
       61 agaattcgcc gatttcgtca tttcaaacgc tgatggccgg aagacgctcc tggatatgct
      121 ggaagggaga ttcatgagtg agcgcctgcg gggatactgc gccgagcctg ctgatgaaac
      181 caattgggcg gtacatgttt tccttggcgt taaccgggat ttatcgaagg agccgtccag
      241 cttgatcttg cttcttgatg agcctgtcac catcgcggga cacccattga catcactgga
      301 aatgcagcta tacggttttg ataattcaat ggctccccaa ggcaaaggca caattaaggt
      361 tgagcttgtc tcgacgtatt cttactggaa ggaactggcg gccaataaaa aggattatga
      421 agcggaaaag caaagggttg ccagtcaagt tatcagcctt ttggaaaaac attttcacgg
      481 gataaccaat caggtggaag tggtcgatgt cccaacgctg ttaacctggg agcgttttat
      541 gggcggaaca catggctttg ttaatgcccc cagcaaaaaa ttggatattc tcggcagctt
      601 gtttaagaat gacgacatga ccatcccggg ccttgataat ttttattttg taggaatgtg
      661 ggccacatct gccggagcaa cttttctcaa tgcgctttcc gggaaaaaag cgatccggag
      721 gatatgccaa aaagaacgaa tcgggtttcg agtaaatgct gagcagttaa aaacgggtaa
      781 tgaaagtcac tgtaatttgt agcgggttca tggatacaaa atttccgaca gcaaggaatc
      841 aattttattc tcatgtatct gacttctaat atgacatact attgtcaaat aagggatggt
      901 atgataagga ctaaaggtgg ttccttaact attagggtga ctacagatgg ttcggagaat
      961 tatttatatg ttcttgattc tcctcgtata gaattggagg catgaatagt catatcttac
     1021 aaaaataaac ggtctagtgg tgttattcaa agttttgcgg gtgacggcgg atcggctcta
     1081 caagccaaac tcaatggacc tgaaggagta tttgttgata gtgcgggcaa tatttatatt
     1141 gccgatacct atacacggtc taaggatgga ctcggaaaac aatctctatt ttgtggattt
     1201 ttacaatcat gttattcgag tgataaggtt ttaaaaggga aattttatga cttggtatac
     1261 gaacattagc caccaataag ttgaagaata acaggggtag tcgagtaaga agagaattga
     1321 gaggttaaga caatgagcaa tgaattcata aatttaaccg cagcaaatat agcaagtgag
     1381 catttgtgtt gtgcgattgc ggataaaaaa catcaacatg gtgttgatct taaaagaaca
     1441 tggcttatag acagaattcc ggaaggtcat atatttcgaa agctaaatga aaaaggcaag
     1501 gtgttcattg aatatgcgcc gcttgaaaaa gcgtgggtac cgattattgg tgataattac
     1561 atctatatat actgcctttg ggtttccggt agtttcaagg gaaaagggta tggtaaagaa
     1621 ttgctggatt attgtatcgc agacgcaaaa cgacaaaata agtcgggcgt ttgcataatt
     1681 agctcaaaag tgaaaaagcc ttttctttca gagaaaaagt tcatgcagaa atttggtttt
     1741 gaaactgtag atacaattgg cagtgagtat gaattgatgg cattgtcact taacggctca
     1801 aaaccgaatt ttgcggcgag tgcaaagaag caaagcatag atagtgaaac actgacgatt
     1861 tattacggtt tgcaatgccc ctacattcca aactgcatcg aacagattga atcttattgc
     1921 aagacgaatg gaattcctgt taaactgatt aaagttgata cgttggaaaa agcgaaagaa
     1981 cttccctgtg tctttaacaa ctgggctgtc ttttataaag gcaagtttga aacggttcat
     2041 ttactcaacg agggtttttt aaagaaaatg cttgacagat gaagatcgag taacatgccg
     2101 tgattcattg tgggaacacc gttatccctg cggtgaaggc ggccggatac attatagggt
     2161 ctttgataac cgggaaattg aggatgttaa agaacttatc aaaatcaaga aaaatccaat
     2221 ccataaggag gggtagtcta gtggcggaga aaaaggtttt ggatgttgta aaagtgggtt
     2281 tttacggaaa taccggggat aatcgttcac cggaggattt cccgtttccg gcctgtatga
     2341 cttcactgat gcagtatttg ggtgaggatt atcctatagt ggaattagaa tcacatcatc
     2401 agaaatatac tcagcgtatc gccaacctgc attttattac tgcctccgga atggcgttcg
     2461 ggttactgtg gagtaaggat tattgtaaga gttgcatgga cttgacccag gtaaacgaac
     2521 ataacgaaac cgttaaaaat gcctttgcct gggcgggata tgaatatgag atcatggaaa
     2581 aagcgtctga cgggagtaac cgcgaggtta tccgggagag aattgttgaa tcgattgata
     2641 agggccttcc ggtgctggcg tttgggattg tcggcccacc cgagtgtgtg atcatcagcg
     2701 gctacgatca taaaggcgaa gaaatcaccg gctggagcca ctttcagcaa tgggaacctt
     2761 gtgagaaaga gtccaatggg atgtttcgca gagccgggtg gtatgattca ctatggaaaa
     2821 ttgtgttcat cggcaaaaaa atcggacgga gcataaacct caaggatgta ctgactctcg
     2881 gattgtctat catggagaaa accgatagcg agggatatgt ggccggtctg gccgtctatg
     2941 acgagtggat tcgttatgtg ttaagcaatg aacttgatac gatagatgat gagacattaa
     3001 gagtgaggca cgatttgcat cacgatttgg ttgggaacct tgctgaagcg cgctgttggg
     3061 gtggggattt tctgcttcac gccgcagagg aaaacaagga tgaaaatatg cgtggcgttg
     3121 caatgtgttt caaaaatatc catgacctgt gctggcaggt atggggcgtg ctcggctcat
     3181 acggacagca ggatgtatgg aagggttttc gaagtaccga aaatcgcagg aaaatcgcca
     3241 agcttttggg tgatatcaaa gaacttgatc aaaaggccat gacgtattta aaattggcga
     3301 taagataaat gattcatatt ccattttatt tccctaaagg aataacttgc ccggcgccga
     3361 aaatagatta ttgcgccggg tttatttttc agcgcgctac attccgggct gtggtaaggg
     3421 aggaagagca agttgaccca gggtgcgacc aagatattag ttggggacga cgaccgggat
     3481 atcgtccggc tcattgccga cagtctggcg gatgaaggat ttcaggttct ctccgcctat
     3541 gatggcgttc aggtattgaa tctgctcgcc cagtccgagg tcggtttatt gattttggac
     3601 attatgatgc cggagatgga tgggctcgag gtctgccgca gaatccgcaa taccacggcg
     3661 gcgccgatca ttctgctcag cgccaaaggc cgcgagctcg acaaagtcgt gggccttgag
     3721 gtcggcgccg acgattatat gaccaagcct ttcagcatca acgagctggt ggccagggtc
     3781 aaagcccatc tgcgccggga gaagcggaat atcccgcaga cccgggagcc caaacgggat
     3841 gtggaacgga tccgtttcga cgacatcgcc atcgataagg atacctttga ggtttggaag
     3901 aatgatgaac cgatcaatct ctccaccaag gagtttcaga ttttgcttta tttgatcgag
     3961 aaccaaaacc tggttgtgag cagggaacag atctatgacg ccatttgggg aaccggcggc
     4021 gattacggcg atctaaacac cgtgacggta catatcaaaa atctccggaa taaactcgat
     4081 ccccaaaacc actacatcaa aacgatatgg ggaatcgggt ataaatttac cggaaccagt
     4141 ggaggcaaca catgaagcgg cgcagcatga aactgagcct gaagtataaa ttgcctctgg
     4201 tgatcttgtc ggtttttttt tgtttatcgg ttgcctagtc gtctattacc ggttgttcct
     4261 catggagcat ctgggcgagg attttgatct cttcaaagac cacctctacc ggatgaatga
     4321 ggatatcgga gcccatctca agacctacta cccggaccgc aatcggatcg gggcctacct
     4381 ggagcggacc gccgttggaa acggggtcaa gatcaccatc tatgacgtgg ccggcaataa
     4441 gattgccgcc agcgatcacc ggaaaggctt tgggctgaat ctggagctaa aaaactttac
     4501 cgtggtcggg gagaatgtca tctatgtggt ggagttggtc tatccattta aggtttcaag
     4561 ttttaccgag ttgaactctt tccggcagat ccgggatgta gccgtagcta tgcttgcaat
     4621 attgatcgtt ttattaatta tctacctcca tttctccctg gtcaggccgt tagctacgtt
     4681 aaacaggggt ttggaaagca tcaattaccg gaatacccgg tttcagattc ccgctgtaac
     4741 ttataaacgc cgcgacgaac tgggcgagct cgccagaaag ttcggagcga tgcaacaacg
     4801 gttggatgca tcctatcgcg aacagagtga gatgatcgcc tccatatccc atgatctgaa
     4861 gaccccgctc acctcgatcc tgggatttct ggaacggtta ttgaaccgga aattgcctcc
     4921 ggaacaagag gatgaatatc accggatcat tcagcaaaaa gcccgggata tcaacgagtt
     4981 ggtaacggag tttaatgact ttgccaccgc cgacctgaag gatgacagac tggaaagcat
     5041 ggctcccgtc gatttgcgca ggtttttctc atcgatctgc gctgaatatc gcagtgagct
     5101 tttgagtagg ggagttcaat ttcaggagac agtccggatc gaaaatggag tgcaaccggc
     5161 tctgcggatc gatcctcaaa agatccggcg cgtctttgcc aatctggtcg gaaattcgtt
     5221 aaaatatggt gataacctga gtcgtatcgg gttcagttgc acggttcggg agaatcaagc
     5281 gattttctca gtggaggata acggccccgg ggttccggag agtgaactca aagccctttt
     5341 tgaaaagttc taccggctcg aaaggtcgcg ttcccgggat aagggaggca gcggattggg
     5401 cctcgccatc tgccggagca tcgtggaaca ttacggggga gagattcgcg cttatctccc
     5461 ggcgggcagc ggtttaggtg ttcgatttgt actgcccctg gacgggcttc ataaatcttc
     5521 ataaagtttt atttttattt cagcacggct tgatactcta agattgaggg tactaggccc
     5581 caagacagat tgttcatcgg tggtaaagga atccagaaga ggagtgcggg ga
//
LOCUS       DASVMB010000551         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_290949,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000551 DASVMB010000000
VERSION     DASVMB010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290949"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VF531_15485"
     CDS             complement(<1..861)
                     /locus_tag="VF531_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7715419.1"
                     /translation="MMYAWEAIQKSLDYIEDNLSEDIKIEALASVAALSPFYFQRLFG
                     RLVKKTVNEYVKLRRLARASEALKNKAKRIMDVAFDCGFSDHANFTRAFKGAYGITPE
                     EYRDHPVILNQFVKPDLVLNYVMVDENVPLITDGIVVEVTRRKLYEPRTFLGIAGQVP
                     ITELVWGRDTGIATAGRIWDDFHRQKRAIPDLFPNGNEFGVLYMGDAPKYCCTYMAGA
                     EAAKESVAEGYTSFTLPCCEYVVCCIEAENFAELIGSALFKAASFMNGWMKKHDLACG
                     DFAAEMYYDTD"
     gene            1207..>3181
                     /locus_tag="VF531_15490"
     CDS             1207..>3181
                     /locus_tag="VF531_15490"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715420.1"
                     /translation="MSIYLLSKRENRRIYPINGRLDLEAWNQQDGPLSLSGGWDFYWE
                     KLLTYRDLKSHPVPDLAVQVPEVWNRYQIKGKNLPGFGYATYRLKVIHVQKGKPLALM
                     IPTFSTSYKLYIDTDLVSANGRVGSGRKQSTPQYLPRSIEFTPAKTSFELIVQVANYT
                     YSRGGMWYALELGTPEQIRRLNRNNADKDLLLIGALSVMALYYLSIFLLRREDKSSLY
                     FVFMCLIFASRVMVYGDYFIYRLIPAIPFQAIIAIAYLSLCWFPIFAVLFVGELFPAE
                     TSSGFKKAALLYGGIMAAMFSFTPIAFFTRLIFLVQMIAMATGFYAIVCLIKAYLNRK
                     KDALLVLGGSLTVIIFAGRDMLYQNNIILSNYGELVQVGLFILLFTQSFVLARRFSEA
                     FRNVEALSRKLLKLDKIKDEFLANTSHELRTPLNGILGITEALLRGGEGEVSSGQKQS
                     LFIIANSCRRLANLINDILDYSKLKYGDIRLHIKPVRLDGLIQTVVKVFQQLSQTKEY
                     EVTADLPSGLPPVMADENRVVQILYNLIGNAAKFTTRGYIKVSAGVNVNMLEVCISDS
                     GEGIPPDKLDDIFKSFEQVDTSLTRKHGGTGLGLSITKQLVELQGGSIWVHSTPGTGS
                     EFYFTLPIAVELPPENETELALPELAVTTVLEPQ"
BASE COUNT          796 a          747 c          780 g          858 t
ORIGIN      
        1 atccgtatca tagtacattt cggccgcgaa atcaccacag gccaggtcgt gctttttcat
       61 ccatccattc ataaaggagg ccgccttgaa aagcgcagaa ccaatcagtt cggcgaagtt
      121 ttccgcttca atgcagcaca cgacatattc gcagcatgga agcgtaaaag aggtatagcc
      181 ttctgcaacg gattctttgg cggcttcggc tccggccatg taggtacagc agtattttgg
      241 cgcgtctccc atatagagaa cgccaaattc attgccgttg ggaaataaat ctggaatggc
      301 ccgtttttga cggtggaagt cgtcccatat cctgccggcc gtggcgatgc cggtgtcccg
      361 gccccataca agttcagtaa tgggcacttg gcccgcaatt ccgagaaaag tgcgcggttc
      421 atataacttt cggcgcgtta cttcaactac gataccgtca gtgatcaacg gaacattttc
      481 atcgaccatg acatagttca ataccaggtc cggcttgaca aactgattga gtatcacagg
      541 atgatcacgg tactcttccg gtgtaattcc gtatgcccct ttaaatgccc gggtgaagtt
      601 ggcgtgatcc gagaatccgc aatcaaacgc cacatccata atgcgttttg ctttgttttt
      661 aagagcttcc gacgctcttg ccagcctgcg cagtttcaca tattcattta cggttttttt
      721 taccaagcgc ccaaacaggc gttgaaaata gaacggcgat aatgccgcga cgcttgcgag
      781 ggcttctatc ttaatatcct ccgacaagtt gtcttcgata tagtccagag atttttggat
      841 tgcttcccat gcgtacatca tcgctcctcc ccgaatataa ggatagcata cgataaactt
      901 ttttgcttgt cacgggatgc tctttttgta gaaaatcgtg tcaccggttg tcccataaag
      961 aaatgtaggt ataagggtct ttttttttaa gccataagcc aattcctgtt cggaactgtc
     1021 gctgaattcg gggtccggcc atagccccta ttggcaggaa attgacgcaa aggaacgaaa
     1081 ttaaccaaca ttaaaagagt tgtattttat acgcgcaata aggggaattg gatgatgacg
     1141 catataacgg caaataagcg gatattattg ctactatcca gcgtaatcat tgccggagtt
     1201 gtccttatga gcatctatct cttatcgaag cgggagaacc gccggatata tccgatcaat
     1261 ggtcgactgg atctcgaagc gtggaatcaa caagacggcc ccttaagtct aagcggcggg
     1321 tgggattttt actgggaaaa gcttctgaca taccgggatc taaagagtca tccggtgccg
     1381 gatcttgcgg ttcaagtgcc ggaagtctgg aacagatatc aaatcaaagg aaaaaacctt
     1441 cccgggttcg gatatgccac ttaccggttg aaggtaattc atgttcaaaa aggaaagccc
     1501 ttagccctaa tgattccgac cttttcaact tcatataagt tgtacatcga caccgatctg
     1561 gtctccgcca acggcagagt cggttccggc agaaaacagt ctacacccca atacctgccc
     1621 cgaagcatcg agtttacccc ggctaaaacc agtttcgaac tgattgtcca ggtagctaac
     1681 tatacttatt ccagaggcgg catgtggtat gccctcgagt tgggcacccc ggaacaaatc
     1741 cggaggctaa atcggaataa tgcggataag gatttactct taatcggggc gctgtcggtg
     1801 atggcccttt attacctgag catcttctta ttacggcgtg aggataagag cagtttgtat
     1861 ttcgtattca tgtgcctgat atttgcaagc cgggtcatgg tatacgggga ctattttatc
     1921 taccggctga ttccggccat tccttttcag gccatcattg ccatagccta tctttcactg
     1981 tgttggtttc ccatctttgc cgtcctgttc gtcggggagt tatttccggc ggagacttcc
     2041 tcagggttca aaaaagcggc tctgctttat ggcgggatta tggcggcaat gttttcgttt
     2101 actccgatcg ctttttttac cagactgatc tttctggtcc aaatgattgc tatggctacc
     2161 ggcttttatg cgatagtttg tttgattaaa gcctacctga atcggaagaa agacgctttg
     2221 ttggtgcttg gcggatcttt aacggttatc atttttgccg gacgtgacat gttgtaccaa
     2281 aataacatca tccttagtaa ttacggtgaa ctggtacagg ttggcttgtt tatcctgctc
     2341 tttacgcaat cctttgtact ggcgcgccgt ttttcggaag ccttccgcaa tgtcgaagcg
     2401 ctatcccgca agctgttgaa gctggataag atcaaagatg aattcctggc caacacttct
     2461 cacgagttac ggacgccgct taatgggatc cttggcatca ccgaggcctt gctgcggggg
     2521 ggcgagggcg aagtaagcag cgggcaaaaa cagagtttat tcatcattgc caatagttgc
     2581 cggagattgg caaatctaat caatgacatc ctggattatt ccaagttgaa gtatggcgac
     2641 atccggttgc atatcaaacc ggtacggttg gacggcttga ttcagaccgt ggttaaagtt
     2701 tttcaacagc tcagccaaac aaaggaatat gaggtcacag cggatttacc gtcagggctg
     2761 ccgccggtga tggcggatga aaaccgggtc gtacagatat tatataatct catcggcaat
     2821 gcggctaaat ttactacccg gggttatatc aaggtttcag ccggggtaaa cgtaaacatg
     2881 ctggaagtgt gtatcagcga cagcggagaa ggaattcccc cggataaatt ggatgacatc
     2941 ttcaaatctt tcgagcaggt ggatacctca ttgacgcgta agcacggagg aaccgggctt
     3001 ggcttatcca ttaccaaaca gttggtggag ttacagggtg ggagcatctg ggttcactca
     3061 actcccggga caggctccga gttttatttt acacttccaa tcgcagtgga attgcccccg
     3121 gaaaatgaaa ccgagcttgc cctgccggaa ctggcggtaa ctactgttct ggagccgcag
     3181 g
//
LOCUS       DASVMB010000552         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_581887,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000552 DASVMB010000000
VERSION     DASVMB010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581887"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..2788)
                     /gene="secA"
                     /locus_tag="VF531_15495"
     CDS             complement(146..2788)
                     /gene="secA"
                     /locus_tag="VF531_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7715421.1"
                     /translation="MLKFLSELWDTNLREVKRLQERVTVINELEPEFQKLSDEELRAK
                     TTEFQERVKNGETLDELLPEAFAIVREAACRTLGQRHYDVQLIGGMVLHEGKIAEMKT
                     GEGKTLVATLPVYLNALSGHGVHVVTVNDYLAKRDSEWMGQIYRFLGLSVGVILHSLT
                     PEERRVAYGSDITYGTNNEFGFDYLRDNMAFSAGDMVQRELNYAIVDEVDSILIDEAR
                     TPLIISGQAEESNDLYYKFAKLVPRLHKDEHYTVDEKARTVAVTEEGVANVEKMLGIE
                     NLYEDSNTELVHHLTQALKAKELMKRDRDYVVKDGEVIIVDEFTGRLMFGRRYSEGLH
                     QAIEAKEGVKIERESQTLATITFQNYFRMYKKLAGMTGTAETEEPEFRKIYNLPVIVI
                     PTNLPMVRVDNPDVIYKTEAGKFKAVVDEITEVHKTGQPILVGTISIEKSEELSNLLK
                     RRGVAHQVLNAKYHEKEAEIIAQAGRFGAVTIATNMAGRGTDIMLGGSPEFLAKDHLR
                     SQGIDPLTASPEEYQAALKEVRTITDKEHEQVVALGGLHIIGTERHESRRIDNQLRGR
                     SGRQGDPGSSRFFVAMEDDLMRLFGGETISGLMERLGWSEDMPIEHPAIAKRIEAAQR
                     KVEARNFEIRKNVLDYDDVLNKQREIIYELRKKLLLGEDVHEKVLDIFEEVTDNLLNT
                     YTNEKVSIDEWDIEGLTHAVYSSLLPPQSLDDEDLEGCNYQELRELILNKSLDAYKAK
                     EEEIGSEYLRQVERFVMLKTIDIHWMNHLEAMDDLKEGIGLRAYGQNDPLVAYKIEAY
                     QMFKEMRDSINEEEIRELTKFQLVSEERVARRPKLRNIATNLNEDGTSAVQQRVVGKK
                     VGRNDLCPCGSGKKYKKCCGREAS"
     gene            3187..3843
                     /locus_tag="VF531_15500"
     CDS             3187..3843
                     /locus_tag="VF531_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7715422.1"
                     /translation="MKLALIIFDMDGLIFDTERLAITAWRLAGADMGFDIPAQVIIET
                     IGTNIQGTEAIFKSRLGPNLPFEQLKALRFDYTWRLMDENGIPVKKGVYELLTLLESK
                     SIPKAVATSTERSKTEELLRRADLIHRFDLLVCGDEVARSKPEPDIFLAAAERLHCVP
                     RECMVLEDSERGIIAASRAGMLPVLVPDIKPPAAEVRTLAHREFPSLLGVAEYIRQIT
                     "
     gene            complement(3927..>4364)
                     /locus_tag="VF531_15505"
     CDS             complement(3927..>4364)
                     /locus_tag="VF531_15505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715423.1"
                     /translation="SSLAVGLFWAAMTLGRIGCGPFILRYSTRTIVIILAFFSAVVTA
                     FSGSTTGNWSWLVIFLMGFAYSSIWPLLVAFGGEQYPVYSGTVFALLVGTGGLGSTII
                     PYLIGIVGQNAGVRIAMISPAVCFLLVALILMGLEKVPRRSIN"
BASE COUNT         1057 a         1213 c          987 g         1107 t
ORIGIN      
        1 tgcctattag caactcaccc ccgacccctc tcttttcgaa aagagaggtc gcgttaaaac
       61 gcggcgtagg gtcaataatg aaagctacaa gtaacgcgga gtgagttgcc tattgcctgt
      121 tccctgttgc ctattgccta atatctcagc tggcctcacg gccacagcat tttttatact
      181 tcttgccact gccgcaaggg caaagatcgt tccggccgac tttcttcccg acgacccgtt
      241 gctgaaccgc ggaagtcccg tcctcattga gattggtggc gatattccga agcttgggac
      301 gtctcgcaac ccgttcctcg gaaaccaatt ggaacttggt taactcccgg atttcctctt
      361 cgttaatgct gtcccgcatc tccttgaaca tctgataggc ctcaatcttg taggccacca
      421 gcggatcgtt ctgtccgtac gcccgaagtc cgataccctc cttgagatcg tccatcgctt
      481 caaggtggtt catccagtgg atatcgatgg tcttcaacat cacgaaccgt tccacctggc
      541 ggagatattc ggaaccaatc tcctcttcct tggctttata agcgtcaagg gatttattca
      601 gaatcaactc gcgcagttcc tggtaattgc agccttccaa gtcttcatca tccaaggatt
      661 gcggcggcag aaggcttgaa tataccgcat gggttagtcc ttcgatgtcc cattcgtcga
      721 tcgatacctt ttcattggta tacgtattta gaaggttatc ggttacctct tcaaatatat
      781 ccagtacctt ctcatggaca tcttcgccca aaagcagctt tttgcggagt tcgtagatga
      841 tctcgcgctg tttgttcagg acatcgtcgt aatccaaaac atttttgcgg atttcaaaat
      901 ttctggcctc caccttacgt tgagcagcct cgatcctttt ggcgatggcc ggatgttcga
      961 tgggcatatc ttcggaccag ccgagccgtt ccatcagccc ggagatagtc tcgccgccga
     1021 agaggcgcat cagatcatct tccatggcta caaagaaccg ggaagaaccg ggatcgccct
     1081 gccgtccgga tctaccgcgt aactggttgt caatccggcg gctctcatgc ctttcagttc
     1141 cgataatgtg taaaccaccc aaagcgacca cttgttcatg ctctttatcg gtaatggtac
     1201 gaacttcttt cagagcagcc tgatactcct cgggacttgc agtcaacgga tcgataccct
     1261 gcgatcgcag atgatccttg gctaaaaact ccggacttcc ccccagcata atatcggtac
     1321 cacggcctgc catgttcgtg gcaatcgtga ccgcgccaaa ccggccggct tgagcgatga
     1381 tttccgcttc cttctcgtgg tatttggcat ttaggacttg atgagcaaca ccccggcgtt
     1441 tcaataaatt actaagttcc tccgatttct cgatggagat cgtacccacc aatatcggct
     1501 gtcccgtctt gtggacctcg gtaatctcgt caaccaccgc tttaaattta cctgcttcag
     1561 tcttataaat cacgtccggg ttgtcgaccc gaaccatcgg cagattggtc ggaatgacga
     1621 tcacgggaag gttatagatc ttccggaact ccggttcttc cgtctcggcg gttccggtca
     1681 tacccgccag tttcttatac atccggaagt agttttgaaa agtaatcgta gcgagcgttt
     1741 ggctttcccg ctcgatcttg acaccctctt tggcctcgat cgcctgatgc agtccctcac
     1801 tgtaacggcg gccgaacatc aatctgccgg taaactcgtc gacgatgatg acctcaccgt
     1861 ctttaaccac ataatcccga tcccgtttca tcaattcctt ggctttcaag gcctgggtca
     1921 gatgatgcac cagttcggta ttggaatctt cataaagatt ctcgatcccc agcatcttct
     1981 cgacattggc cacaccttcc tctgtgaccg ccaccgttct cgccttttca tcaacagtat
     2041 agtgctcatc cttgtgaagt ctgggaacca atttggcaaa tttataataa aggtcattgg
     2101 actcctctgc ctggccggag atgatgagcg gcgttcgcgc ctcatcaatc aagatactgt
     2161 ccacctcatc gacaattgcg taattcaact cccgttgcac catatcgccg gctgagaagg
     2221 ccatattatc gcggaggtag tcaaatccga actcgttatt ggtgccgtat gtgatatcag
     2281 agccatacgc cactcgacgt tcctcgggag ttaatgagtg taaaatcaca cctacgctta
     2341 accccagaaa acggtaaatc tgccccatcc actcgctatc acgctttgcc aaataatcgt
     2401 tgactgtgac aacatgaacg ccatgcccgc tcagggcatt caaataaacc ggcagggttg
     2461 ccacgagggt tttgccttca ccggttttca tctcggcaat cttaccttcg tgcagtacca
     2521 tgcctccgat caactggacg tcgtaatgcc gctggccgag agtacgacag gccgcctcgc
     2581 gtacgatagc aaaagcctca ggcaataact catccagggt ttcgccgttt tttacccgct
     2641 cctgaaactc ggtggttttc gcccgcagtt cttcgtcgct caacttctga aactccggtt
     2701 ccagctcatt gattaccgtc acccgttcct gaagccgctt cacctcgcgg aggttggtat
     2761 cccataattc gctcaaaaac tttaacatct aatcgtctca ctccgatcta cgaactatct
     2821 ttaccaaaga taacgcaatt tttgatgatt agtgccaaaa aagccccaac acccgcaggt
     2881 atgatcaaaa aaacaaaccc gccatgatca cgcgagggcg cgcaagtaca cctggcggca
     2941 acaatttggc agtgtaaatt accgtaatat tataccactc ggatccgaga aaaacaaatc
     3001 caattaaaga atttgggcgg accgaaacgt cggagacggt ttctttgttt ggtaaaaatt
     3061 ctccaactat aattcaaaaa gcctgttcaa ataggtattg gtatccgtcg ctgttcatga
     3121 tatgataaga aacgtattct ttgaagcgga atttataata aaaacattca ggagacctaa
     3181 aaacggatga aacttgccct tatcatcttc gacatggatg gattaatctt cgataccgag
     3241 cggctggcaa tcaccgcgtg gcgtttggcc ggggcggata tgggattcga tatcccggcc
     3301 caagtcatta tcgagaccat cgggaccaat atccaaggca cagaagcaat cttcaaaagt
     3361 cgtttggggc ccaaccttcc cttcgaacaa ttgaaggcct tgcgtttcga ttatacctgg
     3421 cggctcatgg atgaaaacgg cattccggtc aaaaaaggcg tttacgaact tctgacactc
     3481 ttagagtcaa agtcgattcc caaagcggtt gccacctcca ccgaacggag caagaccgaa
     3541 gaacttttgc gccgggcgga tctaattcac cgcttcgatc ttttggtctg cggcgacgag
     3601 gttgcccgga gcaagccgga gccggatata ttcctggccg cagcggagcg cctccattgc
     3661 gttcctcggg aatgtatggt tctcgaggat tcggaacggg ggatcatcgc cgcatcccgg
     3721 gccggtatgc tcccggttct ggttcccgat ataaaaccgc ctgcggcgga ggttaggacc
     3781 ctggcgcacc gggagtttcc ctccctcctg ggtgtagcgg aatatattcg gcaaatcact
     3841 tgatccgatt gctgttgaca accaaaatac aggtagtttc aaccgccaaa tggtttttta
     3901 cacttcaaac ttcactcttc acaatttcaa ttgattgaac ggcggggtac cttctcaagc
     3961 cccattaaaa tcagagccac caagagaaaa cagactgccg ggctgatcat ggctatcctc
     4021 acgccggcat tttgtccgac gattccaatg agatacggga tgatggtgct gcctaaaccc
     4081 ccggttccta ccagaagcgc gaaaaccgtt cctgaataaa ccggatactg ttctccgccg
     4141 aacgccacca gtaacggcca aatactggaa taagcaaatc ccatgagaaa aatcacgagc
     4201 caactccagt ttccggtggt agatccggag aatgcggtaa ccactgccga gaaaaatgca
     4261 aggatgatga cgattgtacg ggtgctgtaa cgcaagataa atgggccgca tccgattctg
     4321 cccagggtca tggcagccca aaacaggcct actgccaggc tcga
//
LOCUS       DASVMB010000553         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_66911,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000553 DASVMB010000000
VERSION     DASVMB010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66911"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VF531_15510"
     CDS             complement(<1..1034)
                     /locus_tag="VF531_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715424.1"
                     /translation="MFRKIGPFILLFLVALSVSTVLAESSFEFQMGDAKDFLSIALSI
                     RPIPTVNPIATGNWTGWNRYTDGQTLSAPAVAVYGDRLCIMARGTDNQLYFKSDAGSG
                     WVLAGGATMSQPALAEYNGVLYALVRGTNNALYINRYQGSWGGWESIGGKALSGGALL
                     ANRGQLFAVVRGSDDAVYVNSMDSRGAWSGWQNCGGSTRYQPALAEFKGSLYVAIRGT
                     DDRIYLKAFNGRSWNNWLEIRTSSPSGVLANGGLAMVEHTSQLWFFTRGNDSRIFYTM
                     LSKDPRAEWREVSGSGRTGESPVAVEYHGGIRLFVRGTDNGIFINSYGGSALPVNPPQ
                     PTRPPQPTPTP"
     gene            1232..2065
                     /locus_tag="VF531_15515"
     CDS             1232..2065
                     /locus_tag="VF531_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX7715425.1"
                     /translation="MIFKAYAVTGEIDLNRIAAQCNLRKKYTWEEPLILQGESLITIL
                     GRGFHKNQQILVFAFGGIVFVNTASKDEKIFINYLKTFKPDLAEADYQTYHENYELRV
                     NRQETEYSDDSPVFTDAYVVVPKLEPHHPELVATVIAKSVALEMTEAEMSRILDRLEE
                     MIDRLERGNLRIGDKALAQATAQVIRHEYNTIAYIMILDKPDITWANSEAALLYDRMA
                     KFFELESRYEIIKQKTDILNVLIGHFSAISHARRSLWIEWLVALLVLAEVILMVADLF
                     K"
     gene            2077..2877
                     /locus_tag="VF531_15520"
     CDS             2077..2877
                     /locus_tag="VF531_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX7715426.1"
                     /translation="MGQNAVISCFKTCPRLDLKQVGNFFKVPVDPGNQSLMISGEQLR
                     PGSEDTAQPKALWIYNYGCICLLGLEEPDTSWIFKTLEASGLPVDYRLAARYQDHHQL
                     ELDADFSCETMESAAVVAAVLAKSTELWKLEDTVDRLVDESERILLELQRGSFRLPDR
                     LLQRMNLDLIRLQYDLIQNLKILDRPPECNYQLDLRELYDQTAEGYEVPERFAIVQRK
                     MADLLAIITPYEDQVYSWKERRVAIMETVLIALFPVSYLIQMIIRRYS"
     gene            complement(3073..3951)
                     /locus_tag="VF531_15525"
     CDS             complement(3073..3951)
                     /locus_tag="VF531_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715427.1"
                     /translation="MKSKQHKLRIMVFLTGLMIAFSGALIQSASEPQYVPLGPESAGL
                     WMQNALVARMVPYRMESNGLQLTVNKLLEWAIDWKQGKFTMKCSFTAGTSGTMLELRQ
                     DGAITLTGSVLFSAPEQKIGFRLLRIDGLELDHTNPFLSEAVRVLIDRGLAGKEFWSG
                     TPPVSSQILTDANFSKLLEMVVSRQLPLEVRDGKSSIVMNRLEEMEFLEEPGKMRVKL
                     NIQGVYHKIFNWKFDGWAQVELRMEVDPSSLAGIVRIEALKDIKLKGTPFWVNHWIRG
                     LINSRIRGQVFRFNWE"
     gene            complement(3968..>4279)
                     /locus_tag="VF531_15530"
     CDS             complement(3968..>4279)
                     /locus_tag="VF531_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715428.1"
                     /translation="QQCAAQGRKYEGLGLAVAHQLWARRLFDQGDWTMAIFHSLRSRI
                     LAARVITSNQSDLLSEALYDQQEQKFAQATPTSQELDQKLRESKIEVPNDPAAVGVQV
                     R"
BASE COUNT         1113 a         1112 c         1011 g         1043 t
ORIGIN      
        1 ggagtgggag tgggttgggg aggccgggtg ggttgcggcg ggtttaccgg aagagccgaa
       61 ccgccatatg aattaatgaa gatcccgtta tccgttcccc tgacgaataa gcgaattccg
      121 ccgtgatatt ctactgctac cggagactca ccggttctac cgcttccgga aacctcgcgc
      181 cactccgcac gaggatcctt agaaagcatc gtgtaaaaaa ttctggaatc gttccccctg
      241 gtaaagaacc ataattgact ggtatgctcc accattgcca atccaccatt cgccaagaca
      301 cccgatggag aactggtgcg aatctcgagc caattattcc atgacctgcc gttaaatgcc
      361 ttcaagtaga tccgatcatc cgttccccgt atcgcaacat acaaagaacc tttgaactcc
      421 gccagagcgg gttgataccg ggtggagccg ccgcaattct gccaaccgct ccaagctccc
      481 cgggaatcca tggaattgac atagactgcg tcatcgcttc cccggacaac ggcaaataac
      541 tgtccccgat tggcaagaag ggcaccgccg gataatgcct tgccgccgat actttcccac
      601 ccgccccagg atccttgata cctgtttata tacagtgcat tgttggttcc gcgaaccaat
      661 gcgtacaaaa caccgttata ttcggcgaga gccggctggg acatggtggc gccacctgcg
      721 aggacccacc ctgaaccggc atcggattta aagtagagtt gattatcggt tccccgggcc
      781 atgatgcaaa gcctgtctcc atacactgct actgccggag cggataatgt ctgtccgtcc
      841 gtataacggt tccatccggt ccaatttccc gttgcaatcg gatttaccgt tggaatcgga
      901 cgaatcgaca aggcaatgct caaaaaatcc ttggcgtccc ccatctgaaa ctcaaaggag
      961 gattcggcca aaacggtcga aacactcaga gcaaccagga ataataggat aaacgggcca
     1021 attttacgaa acatgtggat gacctcctcg ggattttatc ttgttgagtt cattttaact
     1081 cccggtgatt aaataaaaat ataaaaggat gaagatttct taaaaaataa tgtagggtca
     1141 ttccatagat cgtagccacg ttccatggaa cggcggtaca tggatattcc tagtcatagg
     1201 ttatgatata atgatcttgg aggtgttctg cgtgattttt aaagcctatg ccgtaacagg
     1261 tgagatcgac ctcaaccgga tcgcggccca atgcaacctc cgcaaaaagt acacctggga
     1321 agagcccttg atcttgcagg gtgaatcact cattaccatc ctgggacgcg ggtttcataa
     1381 gaaccaacaa atcctggtct tcgcctttgg cggaatcgtc tttgtcaaca ccgcgagcaa
     1441 ggatgagaag attttcatta actatttaaa gactttcaaa ccggatctgg ctgaggcaga
     1501 ttatcaaact tatcatgaga attacgagtt gcgcgttaac cggcaggaaa cggaatattc
     1561 cgacgacagt ccggttttta ccgatgccta tgttgtcgtg ccgaaactcg aacctcatca
     1621 tccggaattg gtggcaacag tcatcgccaa atcagtggcc ctggaaatga ccgaagccga
     1681 aatgagccgg atcctggatc gcctggagga gatgatcgac cgcctggaaa ggggtaacct
     1741 ccggatcggc gacaaagctt tggctcaggc caccgcgcaa gtcatccgcc acgaatataa
     1801 caccatcgcc tacatcatga tcctcgataa accggatatt acctgggcta atagcgaagc
     1861 cgcgcttctt tatgaccgga tggccaagtt ttttgaactg gagagcagat acgagattat
     1921 caaacaaaaa accgatatcc tgaatgtgct gatcggccat ttctccgcca tcagccatgc
     1981 tcgccgcagc ctatggattg agtggctggt cgctctgttg gtcttggccg aagttatcct
     2041 gatggtggcg gatctcttta agtaggagcg ggaataatgg gacaaaacgc agtaatcagc
     2101 tgttttaaaa cctgcccacg gttggatcta aagcaggtgg gaaatttctt caaagttccc
     2161 gttgaccccg gaaaccagtc gttaatgatc tcaggggaac agctccgccc tggttccgag
     2221 gataccgccc aacccaaggc cctatggatt tacaattacg gttgcatctg cctgttaggc
     2281 ttggaggagc cggacacctc ctggattttc aaaaccctgg aggcttcggg gctccccgtt
     2341 gattaccggc tcgccgccag gtatcaggat caccaccagt tggaattgga cgccgacttt
     2401 tcctgcgaga cgatggaaag tgccgcggta gtcgctgctg tgctggccaa atccaccgaa
     2461 ctatggaagc tggaggatac ggtagatcgt ttggtcgatg aatcggaaag gatacttctc
     2521 gagcttcagc ggggctcgtt ccgtctcccc gatcggttat tgcagcggat gaacttggat
     2581 ctcatacgtt tacagtacga tctgatccaa aacctcaaaa tactcgatcg gccgccggag
     2641 tgtaactatc aactggatct acgggaatta tacgatcaaa ccgccgaagg ttacgaggta
     2701 cctgaacggt ttgctatcgt acagcgtaaa atggctgatc tactggcaat cattaccccc
     2761 tatgaagatc aggtatacag ttggaaggaa cggcgagtgg cgatcatgga gaccgtgctt
     2821 atcgctcttt tccctgtctc atacctgatt cagatgatta ttcggcgcta ttcataagtc
     2881 aaaagtctaa aaatcaaaag gattgagtca aaagtcagaa gtcgaaaact caaaacataa
     2941 tagtgaattt ggattacagc cttgaacttt gaactttgaa ctttgaactc tgaactctga
     3001 actctgaact ttgaactttg aactttgaac tccggacttt agactgtaga ctgtagacta
     3061 aagatttaag cctcactccc aattgaatct gaatacctga ccacggatcc gggaattaat
     3121 caggccgcga atccaatggt taacccaaaa cggagtccct ttcagtttaa tatcctttaa
     3181 agcttcgatc cgcacaatcc ctgccagact tgacggatca acctccatcc ttagctcaac
     3241 ctgagcccag ccatcaaact tccagttaaa gatcttgtga taaacccctt gaatatttaa
     3301 tttaacccgc atttttccgg gttcctccaa gaactccatc tcttccaggc gattcatgac
     3361 gatcgaggac ttcccgtctc ttacctctag aggcaattgc cgggatacga ccatctccag
     3421 caacttggag aaattggcat ccgtaagaat ttggctagat acagggggtg taccagacca
     3481 aaattccttc ccggccaggc cacggtctat aagcactcgg acggcctcac tcaggaatgg
     3541 gtttgtatga tcaagttcca agccgtcgat gcggagtaac cggaagccga ttttttgttc
     3601 gggggcggaa aaaagtacac taccggttaa ggtgattgca ccatcctgtc tgagttccaa
     3661 cattgtaccg gaggtacccg ccgtgaaaga gcatttcatg gtgaactttc cctgtttcca
     3721 gtcaatggcc cactcgagca acttatttac ggttaattgc aacccgtttg actccatccg
     3781 ataaggaacc atccgggcca ccaaagcatt ttgcatccaa agcccggcac tctccgggcc
     3841 tagcggaaca tattggggtt cggatgcgct ttgaatcaag gctccactaa aagctatcat
     3901 cagtccggtt aaaaatacca ttatccggag cttgtgttgt ttggacttca tagtgccctc
     3961 ctcccgccta tcgaacttga accccaacgg cagccgggtc gtttggtacc tcaatcttcg
     4021 attcccggag tttttgatcc agttcctgac tggtaggggt tgcctgagca aatttctgtt
     4081 cttgctgatc gtaaagggcc tcgctcaaca aatcactctg gttggaggtt atgacgcggg
     4141 cggccaaaat ccgggagcgt aaagaatgaa agatcgccat cgtccaatcc ccctggtcaa
     4201 acaggcgtct tgcccaaagt tgatgtgcca ccgccaatcc caaaccttca tattttctcc
     4261 cctgagccgc acattgctg
//
LOCUS       DASVMB010000554         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_581968,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000554 DASVMB010000000
VERSION     DASVMB010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581968"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VF531_15535"
     CDS             <1..756
                     /locus_tag="VF531_15535"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HEX7715429.1"
                     /translation="ASSQEQGSPVVLTTPQWKKKKLNSTLASWAELKHDTILYGKQTG
                     AECGGGDELPKVYGYIEPELQFYRALRQTIVAMDRDLKQMNAAGIHNSEQLLELIDFF
                     ILTSEKELKQIPLSDQENEQIRIIGGLLEANTINALDAQMRWWEITSESAKNMAVTAD
                     IFSFWGQYQTEAVGYGDDLYILIPVNGQLYLMRGSIFSYYEFLNQKRMTDSEWYELLK
                     TIKIEKRPKWWQPYLDGKKAEIPVPADPYDSGC"
     gene            681..1742
                     /locus_tag="VF531_15540"
     CDS             681..1742
                     /locus_tag="VF531_15540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7715430.1"
                     /translation="MVATVPGWKKGRNPGPGRSLRFGLLIPILFLGSFGWTRPSEIKL
                     GFIGDILPDRGVKSVIQKIGLAGLFQNAAALFQDTDLVCANLEAAITTSDTPLVKTFR
                     FNSPLNLGEYLIKNKVKVVTIANNHSIDYGKAGLKDTVANLAKYQVEAFGYGDNAQAA
                     LAPKIIELNGTRIAFVGAVCFPLEGYVYLPNSFDVGRWDAQLMKFQIQAARRQADIVV
                     VSLHWGIEFSHYPTASQIEIGHYCIDQGADLVMGHHPHVLQGMEIYHHKPIFYSLGNF
                     IFDQHYGPTTETVFAKVTIARKKINRIELTPVFINESVPERADEKKATTIRSNFIKYS
                     EPFGLTDSTFKNFELTDFE"
     gene            complement(1751..2338)
                     /locus_tag="VF531_15545"
     CDS             complement(1751..2338)
                     /locus_tag="VF531_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7715431.1"
                     /translation="MQTLPLAITLFFVLDPFGNLPVVLGLLTHIEPKRRLKIVLRESI
                     IALLLLVVFYAVGPWFLNILEIGSSDLKICGGVVLALIALRMVFPDERLVSSQEDQSE
                     PFIVPMAIPLMVGPSALATVMIMAAQSASQPLIGLMMMGIAWLLTAVLLLAGVRLGSL
                     IPPRLLIALERLAGLLLALIAVHMLMSGIQTYLVK"
     gene            complement(2420..4060)
                     /locus_tag="VF531_15550"
     CDS             complement(2420..4060)
                     /locus_tag="VF531_15550"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7715432.1"
                     /translation="MKRNLLVSIAGLCLVVCFLLPLVLASNKESSEIKEATIVNAYWA
                     GDYQVPDISPRRNNSFMLEIQFPMDNPKPEQIHNFLVKAGYQFQLKAKGEKTLNVLGY
                     ECGYYQLSNKNIWGYWFFIDSKEPVSIRTDIPYQLTLKKPNKDYRWMIKGKITFRNLN
                     DVQFSTYKDPGSEILTHGKPWGSMVTATIPFSNTGGLVIVKTRINHSDKEYRFAVDTG
                     AGPIVIDNKLANELGLVKKAEGKVADYFDTKSVSIVSLDRLTVGETGVENCGAVVMSL
                     QVLGGYRVDGLIGYDFLKFFNVKLDYPQQKLTLSRENIPSAPSSYEIKLHLKPNRQIA
                     TQLNIGGIGVEATIDSGALGDYYLIIPIAFFDQNPSRFGCKPLKIKGIAAQGPVSQSE
                     GMVSRINSIQLGDFQVNNLPVICEQSDNFVLGSAFLSHFEVTINFPALEMNLVPIPSD
                     KMSDNILAYGFGSQKDDAGKIHITGIIEGSPADLAGLKTGDEIEEIHNAENRELSYEE
                     SVNLIASGDNRQIKLFIRDRDGKREVVLNKALLLPDVK"
     gene            complement(4132..4431)
                     /locus_tag="VF531_15555"
     CDS             complement(4132..4431)
                     /locus_tag="VF531_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7715433.1"
                     /translation="MDLSSLPEAFESRIRVAVLAILSKDQSKSFSDLKAITGAADGNL
                     SVHLRRLEEAGYITIQKRFANLRPQTIYTITERGKNEFREYIDFLGKLIAEDEKS"
     gene            complement(4409..>4689)
                     /locus_tag="VF531_15560"
     CDS             complement(4409..>4689)
                     /locus_tag="VF531_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715434.1"
                     /translation="TTVSPDNMNVAILVALVATGVLINKGYFYKLTVCCIVLNWVLRC
                     LPETWRQLSYISGIVNLLIPLWGTGIYFLWQKRKMLAKGNSDGFEQSA"
BASE COUNT         1320 a         1124 c         1014 g         1231 t
ORIGIN      
        1 gccagtagcc aggaacaagg atcaccggta gtacttacca ccccccaatg gaagaagaaa
       61 aagttaaatt caacactcgc cagttgggcc gagttaaaac atgatacgat tctctatggg
      121 aaacaaacgg gggcggaatg cggcggcggg gatgaacttc ccaaagtata cgggtacatc
      181 gaacccgagc ttcaatttta ccgggcttta cgccaaacca ttgtcgccat ggatcgagat
      241 ctgaagcaaa tgaatgctgc cgggatacat aattcggagc aattattgga attaatcgac
      301 ttctttatcc tgacgagcga gaaggaactg aagcaaattc ccctgagcga tcaggaaaat
      361 gagcaaatcc ggattatcgg cggtttattg gaagccaaca ccattaacgc tttggatgcg
      421 cagatgcgct ggtgggagat tacttcggaa agcgccaaaa acatggcggt aacggccgat
      481 atttttagct tttggggcca gtatcaaacc gaagcggttg gttacggaga tgatctctat
      541 attctcattc cggtgaatgg ccagttatat ctgatgcgtg gttcgatctt ctcgtactac
      601 gaatttctga accaaaaacg aatgaccgat tcggagtggt atgaactgct taagacgatc
      661 aaaatcgaaa agaggcccaa atggtggcaa ccgtacctgg atggaaaaaa ggcagaaatc
      721 ccggtcccgg ccgatcccta cgattcgggc tgctaattcc gatactgttt ttggggagtt
      781 tcggttggac aagaccgtcc gaaatcaaat tgggctttat cggagacatt cttccggatc
      841 gcggcgtcaa aagtgtgatt caaaagattg gcctggcggg attatttcaa aacgccgcgg
      901 cgctttttca ggataccgat ctggtctgtg ccaatttgga agcggcgata accacctcag
      961 atacgccctt ggttaagact tttcggttta attctccgtt gaacctcggt gagtatttga
     1021 taaaaaataa ggttaaagtc gttaccatag ccaacaacca ctccattgat tacggtaaag
     1081 ccgggctaaa agataccgtg gcgaacttag ccaaatatca agttgaagct tttggttacg
     1141 gggataatgc ccaagccgct ctggcaccga agatcattga gcttaatggg actaggatcg
     1201 cttttgtcgg agcggtttgc tttcccttgg aagggtatgt atatttgcct aattcctttg
     1261 atgtcggccg ttgggatgct cagttgatga agtttcaaat tcaagctgcg cgacgccagg
     1321 ccgatattgt ggttgtcagt ctccactggg gtatagagtt ttcccactat ccgaccgctt
     1381 cccagatcga aatcggccat tattgtattg atcaaggcgc tgatttggtc atgggacatc
     1441 atccccacgt attacaagga atggagattt atcatcacaa acctatcttt tacagtctgg
     1501 gtaattttat tttcgatcaa cattacggtc caaccaccga aacagttttc gccaaagtga
     1561 cgatcgcaag aaaaaagatc aatcgtattg aactgacacc ggtttttatc aacgaatccg
     1621 taccggagcg ggcggatgag aaaaaagcca ctaccattag aagtaatttt atcaagtatt
     1681 cggaaccctt tggcttaacc gactctacat tcaaaaactt tgaattgacg gactttgagt
     1741 agacctaaat ttatttaacc agatacgtct gaatcccgct cattagcata tgcaccgcaa
     1801 tcagcgccaa taataacccg gccaaccgtt ccagcgcaat caacaaacgc ggcgggatca
     1861 gtgaacccaa acgtactccc gccagtagca gtaccgccgt caacagccag gcgatcccca
     1921 tcatcatcaa gccaataagc ggctgcgagg cggactgggc agccatgatc atcaccgtag
     1981 ccagggccga cggcccgacc atcaacggaa tcgccatggg aacaatgaaa ggttcactct
     2041 gatcttcttg gctactcacg aggcgttcgt ccggaaatac catccgtagc gcgataagtg
     2101 ccaaaaccac cccgccgcag atcttcaaat ccgaggatcc gatctccagg atgtttaaaa
     2161 accatggacc aacggcatag aaaaccacca atagcagtag cgcaatgatg gattcccgca
     2221 gaacgatttt caggcggcgc tttggttcaa tgtgtgttaa tagcccgagc accaccggga
     2281 gatttccgaa aggatccagc acaaaaaaga gggtgatagc gagcggcaaa gtttgcataa
     2341 aatgagtcac tcctatgatg tgataaaaaa agattctccc aattttcgag agaacctttt
     2401 tctatttcga ttcaggaatc tattttacgt ccggaagcaa cagggcctta ttcaaaacca
     2461 cttccctctt tccatcccgg tctctgatga agagcttaat ttgtcgatta tctccggagg
     2521 caataagatt tactgattcc tcataactca gctccctatt ctcagcatta tgaatctcct
     2581 caatttcatc accggttttc aaaccggcca aatcggccgg ggagccctca atgattccgg
     2641 taatatgaat cttgccggca tcgtcttttt gcgacccgaa tccatatgcg agaatattgt
     2701 cgctcatttt atcactggga atgggtacta aattcatttc caaagccggg aaatttatgg
     2761 ttacctcaaa atgagataaa aatgcactac ctagtacgaa attgtcggac tgctcacaaa
     2821 taacaggcag attgttcact tgaaaatctc ccagttggat cgagttgatt cgactaacca
     2881 tcccctcaga ctgactaaca ggtccctgag ccgcgatgcc ctttattttt aacggtttac
     2941 acccgaatcg ggatggattt tgatcgaaga atgctatcgg gattatcagg taataatctc
     3001 ctaatgcgcc gctatcaatc gtcgcttcaa ccccgattcc cccgatattt aactgggttg
     3061 cgatttgccg gtttggtttc agatgtaatt taatttcata agatgacggg gcggacggaa
     3121 tattttcccg ggaaagagtt aacttttgct gcggataatc cagttttaca ttgaaaaatt
     3181 ttaaaaagtc atacccgatg agtccatcga cacgatagcc gcccagaact tgcagactca
     3241 tcaccaccgc gccgcaattt tcaactcccg tctcccccac tgtcaagcgg tcgagcgaca
     3301 caatcgaaac agatttcgtg tcaaaataat ccgccacttt cccctccgcc tttttgacca
     3361 accccaattc attggccagt ttattatcaa tgacgatggg gcctgccccg gtatcgactg
     3421 caaagcggta ttctttatcg ctatgattga ttcgggtttt gacgatcact aaaccgccgg
     3481 tattgctaaa tggaatggtg gctgtaacca tggatcccca cggttttcca tgggttaaaa
     3541 tttcagagcc gggatcttta taggtactga actggacgtc gtttaaatta cgaaaagtaa
     3601 tcttaccctt tatcatccag cgataatctt tgttcggttt tttaagcgtt aattgataag
     3661 gaatatccgt tctgatggat actggttctt tactgtcaat gaagaaccaa tagccccaaa
     3721 tgttcttatt gctcaattga taataaccgc attcataccc taagacattc aaggtttttt
     3781 cacccttggc cttaagctga aactgatacc cggctttcac gaggaaatta tgaatctgtt
     3841 cgggcttcgg gttgtccatg ggaaattgaa tctccaacat aaaactgttg tttctgcggg
     3901 gcgaaatatc gggaacttga taatcgcctg cccaataggc gttgacgatt gtggcttcct
     3961 tgatttcact ggactcctta ttacttgcca gtaccagtgg taataaaaaa cacaccacta
     4021 aacacaaacc ggcaatggaa accagtaaat tccttttcat tttcaaacct ccttggtaga
     4081 cccggaaaca taagcacaat tccgaatcaa atgaaattga accgggtaaa attacgattt
     4141 ttcgtcttca gcgattaatt tccctaagaa atcgatatat tccctaaact cattcttccc
     4201 ccgctcggtg atggtatata tcgtttgggg gcggagattg gcgaatctct tttgaatcgt
     4261 aatatacccg gcctcctcca aacgccgcag atgaacgctg agattgccat cggctgcccc
     4321 ggtaatcgcc ttcaaatcac taaagctttt actctggtcc ttgcttagga tcgccaagac
     4381 agccacccgg atccgggact caaaagcctc aggcagactg ctcaaatcca tcggagtttc
     4441 ctttcgcgag cattttgcgt ttttgccata gaaagtatat ccccgtaccc cataatggta
     4501 ttaaaagatt tacgatacca ctgatatagg aaagttgccg ccatgtttcg ggtaaacatc
     4561 tcaacaccca attcaaaacg atacaacaaa cagtcaattt ataaaaatag cccttattaa
     4621 tgagtactcc ggtggcaacg agtgcaacca aaatagcaac attcatatta tccggcgaaa
     4681 ctgtggtca
//
LOCUS       DASVMB010000555         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_581997,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000555 DASVMB010000000
VERSION     DASVMB010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581997"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF531_15565"
     CDS             <1..267
                     /locus_tag="VF531_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715435.1"
                     /translation="NPPQVTGIVAGIVNSGAFIGAGLMQPLFGWVLDRHWQGAMVQGV
                     RVYPLHAYQSAFWFCAVVLGLGIGFSLLVKETKCVNITLAKNDG"
     gene            460..1110
                     /locus_tag="VF531_15570"
     CDS             460..1110
                     /locus_tag="VF531_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7715436.1"
                     /translation="MTIGSPADGNTIHAETQTRYRQMAGDSCNLSCGNNLAFLDLIPG
                     NHLLDLGCGRGLETIRAAKLVGRTGLAVGLDLTPEMIAKAVENAAREGITNVHFMAGE
                     IERLIFEDQSFDAVISNCAINHARNKTMVFHEIHRILKPTGKMVISDAVSKIPLPEHI
                     KNDPEAWAACFGGAVTEWEYLQSIKTAGFDNIEILKRREYLKNGYDFISLTIRATP"
     gene            1563..3695
                     /locus_tag="VF531_15575"
     CDS             1563..3695
                     /locus_tag="VF531_15575"
                     /inference="COORDINATES: protein motif:HMM:NF040157.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HEX7715437.1"
                     /translation="MMIYGKNNVIVPIRKANGSPEEYAIFNPLSGSFDLMNREEYDWL
                     SALKETGRTDPAHRKFYEYLLERGYLFADKTAEEARIIQEFAKFQAEVTGSQTQLLLI
                     PSYGCNLACTYCYEQGVAGEAGLITKETVDAFFDYVGRNFTAPDKPKPFVTLFGGEPL
                     INTPAHREMIRYIVDHAASFGYELAVVTNGYALAEYVEILNRAKIKEIQVTLDGSKAV
                     HDKRRGTRNGKGSFDKIVSGIREAVQNRFPINLRTVVDQENLMDLVNLAEFLNDQGWL
                     DLGPERFKTQIGRNYELFTCYAKPEHLLSQVELWAEFVKLSRDYPVLRKFHRPDFKGI
                     RHLVDTGSLYFASFDTCPAAKREWVFDLHGAIYGCTASCGREEYRLGSYYPEVRLNSE
                     RIGEWQKRNVLTIPECRECPTNVLCGGGCGVIAANKTGRVLSPDCRPVRELIDLGINY
                     YSDEILKMAGEGHDHTTGCRICGAELVYQEHSFQAHCEICGLQLDTSVSCKNGHYVCD
                     GCHRGDILSRVERICRETELQDPVLLAQEIFRLPGLNMHGPEYHSIVPAIIVAAYENT
                     IHNKRFGDIRTAIQRGKAIPGGTSGSHGACGAAIGLGIAYSILNQVTPLSRESRGQAN
                     RLTAAALTAIAKYDGPRCCKREAVGVLETAKQFLPGFNGLPQSSYVCSQFSKNKGCLG
                     ERCPYFPVRDQEEAECEACCQSRKDDFQ"
     gene            3692..>4562
                     /locus_tag="VF531_15580"
     CDS             3692..>4562
                     /locus_tag="VF531_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7715438.1"
                     /translation="MSDAISYINADQFEAEVLQSGRPVIVDFYSEECPPCEVLAPIYE
                     KMAEKYGTHLKFIKIHRQANRELAKSLNVSSSPTVLFFKNGQEVGIRLNGFLSKPQVR
                     KAIEAVLGDLLPAVPLRSEAYDVMILGAGAAGLAAAIYAARAKMKTVVIDESVPGGQA
                     ASTYHVANYPGTPGVIRGKELIRNMREQAVSFGAVIEDLKEVFEVRLAGPEKYIRTED
                     VEYHGKVVIIASGAQPRALPAEGAAEFKGRGVHYCATCDGAMYQDRRVVVVGGGNSAV
                     EEALFLTRFASRVT"
BASE COUNT         1095 a         1088 c         1318 g         1061 t
ORIGIN      
        1 aatccgccgc aggttacggg gattgtggcc ggtattgtca attcgggcgc ctttatcggg
       61 gccggattga tgcagccctt atttggctgg gtacttgatc gccactggca gggtgcgatg
      121 gtgcagggtg tcagagttta cccgctacac gcctaccaaa gcgccttttg gttttgcgct
      181 gtcgtcttgg ggctggggat tgggttttcc ttgctggtta aggagacaaa gtgtgtcaat
      241 attacgctag caaaaaatga tggatgatgg ccaacaggca atatctcatc ctattactgt
      301 ttaatcaata agggctgtga ccgttactaa cgggccggcc tttttttgat ggtggagtcc
      361 acagttatta caatccgaga ttattgtctt gacttgccgg aaatatattg acaatgatat
      421 gaaattgaat tactattata ccataggggg tatggtataa tgacaattgg atcaccagcc
      481 gatggaaaca caattcatgc agagacccaa acccgttacc ggcaaatggc gggtgacagc
      541 tgtaatctga gttgcggcaa taacttggct tttttggatt tgataccggg aaatcatctt
      601 ctagacctgg ggtgcggcag gggactcgag acaatcaggg ccgcaaaact ggtcggccgg
      661 accgggctgg ccgtcgggct tgacctcact ccggagatga tcgccaaagc tgtagagaat
      721 gccgctcggg agggtatcac caatgttcat ttcatggctg gcgagattga gcggttgatc
      781 ttcgaggatc aaagctttga tgcggttatc agtaattgcg ccattaacca tgcccgtaat
      841 aaaaccatgg tgttccatga gattcaccgg atacttaaac ccaccggcaa gatggttatc
      901 tccgacgcgg tgagtaaaat tcctctgccg gagcatatca aaaacgaccc ggaggcttgg
      961 gctgcctgct tcggaggggc ggtaaccgag tgggagtact tgcaaagcat taagaccgcg
     1021 ggatttgaca acatcgaaat cttgaaacga cgcgagtatc tgaaaaacgg ttatgacttt
     1081 atcagcctta cgatacgagc tacaccctaa cttatttatt ttctcgggga agggggtgat
     1141 tcgtgatgaa accggttact cgaggggcag gcggcaattc ctgctgcaat gattccaaaa
     1201 cagcgcaatg ctgctccaag tcgtcgaaag tgcttgaagg atgccatgat tgattaggca
     1261 acagggaaca ggcaataggc aacaggcaac agatgtaggt gtttcgatga agatgagtca
     1321 ttgcgttcgc cagcgtaagg caactcaccc cgcgtcttcc tggtgccatc agtaaaaccc
     1381 tacacccctc tctttctgaa aagagagggg cctggggtga gttgctaatc gacttatggc
     1441 gcaataatta cggactgggg aaggcaacag ggaacaggca ataggcaaca ttggacttta
     1501 gatttaccct ttctaatcct attgcccact gcctgttgtc taattgtaga acgggggttc
     1561 gaatgatgat atacggcaaa aataatgtaa ttgttccgat tcgcaaggca aacggttctc
     1621 ccgaggagta tgcgatattc aaccccttaa gcggcagttt tgatctgatg aaccgagagg
     1681 aatatgattg gttatcggct ttgaaagaaa ccggccgaac cgatccggcc catcgcaaat
     1741 tctacgaata tcttttggaa agaggatatt tgtttgccga taaaacggct gaggaggccc
     1801 gaatcattca ggagttcgcc aagttccagg cggaagtgac cggctcccag acccaactgt
     1861 tactcatccc ttcctatggt tgcaacctgg cttgcaccta ctgttatgag cagggcgtgg
     1921 ccggtgaagc cgggttgatc accaaggaga cggtagatgc cttcttcgac tatgtcgggc
     1981 ggaatttcac cgcccccgat aaaccgaagc cctttgttac gcttttcggc ggggaaccat
     2041 tgattaacac cccggcccac cgggagatga tccgctacat cgtggatcac gccgcctcgt
     2101 ttgggtacga gttggccgtg gtcaccaacg gctatgccct ggccgagtat gtcgaaatct
     2161 tgaaccgtgc caaaataaaa gagattcagg taaccctgga cggcagtaag gctgtgcacg
     2221 acaagcgccg ggggacccgt aacggaaaag gttcatttga caagattgtt tcggggattc
     2281 gcgaagcggt ccaaaaccgt tttcctatca acctacgtac tgtggtcgat caagagaatc
     2341 tgatggatct ggtcaacctg gccgaattcc tcaatgacca aggctggctg gacctggggc
     2401 cggagcgctt taaaacccag attgggcgaa attatgagtt attcacttgc tacgccaaac
     2461 cggaacactt gctgtcccaa gtggaattat gggctgaatt tgtgaaactc agtcgggact
     2521 atccggtctt gcggaagttt caccgtccgg atttcaaggg cattcggcat ctggtcgaca
     2581 ccgggtcact ctattttgcc agttttgata cttgtccggc cgccaaaagg gaatgggtct
     2641 ttgatttgca tggcgcgatt tacggctgca ccgccagttg cggccgggag gagtaccggc
     2701 tcggcagtta ttatccggag gtacgcctaa attccgaacg gatcggggag tggcaaaagc
     2761 gcaacgtgct taccattccc gaatgccggg aatgcccgac taatgtcctt tgcggcgggg
     2821 gctgcggggt cattgccgcg aataaaaccg gtcgggtgtt gagccccgat tgccgcccgg
     2881 tccgggagct aatcgatctg ggaatcaact attacagcga tgagattctt aaaatggccg
     2941 gggaggggca tgaccataca accggttgcc ggatctgcgg cgcggaattg gtttatcagg
     3001 aacactcgtt tcaagcgcat tgtgaaatct gcggcctgca gttggacacc agtgtttcct
     3061 gtaaaaacgg gcattatgtt tgcgatgggt gtcaccgcgg tgacatttta agccgggtgg
     3121 agcggatctg tcgggaaacg gaactgcagg atccggtatt gcttgctcag gagatcttcc
     3181 gcttaccggg attaaacatg catggcccgg agtaccacag catcgtgcct gcaataatcg
     3241 tggccgccta tgagaatact atccacaata aaaggtttgg ggatatccgg accgccatcc
     3301 aaagagggaa agcgattccg ggaggaacca gcgggagcca cggcgcttgc ggcgcagcca
     3361 tcggactggg tatcgcctat tccatcctga atcaggtcac gcccctttcc cgggagtcac
     3421 gcggtcaagc aaaccggctt accgctgcgg ccctgacggc gatcgccaaa tatgacgggc
     3481 cccgctgttg taaacgggag gcggttgggg ttttggagac ggccaagcaa tttttacccg
     3541 gtttcaacgg gttgccccaa tcttcatacg tatgtagcca attctcgaaa aataagggat
     3601 gtctggggga gcggtgcccg tattttcccg tccgggatca agaagaagcg gaatgcgaag
     3661 cgtgttgtca gtcaagaaag gatgatttcc aatgagtgat gccatctcct atatcaatgc
     3721 cgaccagttt gaagcggagg tattgcaaag cgggaggccg gtgatcgtag atttttattc
     3781 cgaggaatgc ccgccctgtg aagtgctggc accgatctac gaaaaaatgg cggagaaata
     3841 cggaacccat ctgaagttca tcaagatcca ccgccaggcc aaccgtgaat tggccaagag
     3901 tctcaatgtg agcagcagcc cgacggtgct tttctttaag aacggacaag aagttggtat
     3961 acgcctcaac ggttttctaa gtaagcccca agtccgaaag gccattgaag cggtattggg
     4021 cgatctgctc ccggcggttc cgctgcgcag tgaggcctat gatgtcatga ttttgggagc
     4081 cggggccgca gggttggctg cggcgatata cgcagccaga gccaagatga agacggtggt
     4141 gattgatgaa agcgttccgg gcggccaggc cgcttccacc tatcatgtag ccaattatcc
     4201 cggaactccc ggggtgatcc ggggcaagga attgattcgg aatatgaggg aacaggctgt
     4261 cagtttcgga gcggtgattg aagatttaaa agaggttttt gaggtgcgac tggctggccc
     4321 ggaaaaatat atccgtaccg aagatgtgga ataccacggt aaagtagtca ttatcgcctc
     4381 cggcgcccag ccccgggccc ttcccgccga gggagcggct gagttcaagg gccggggtgt
     4441 tcattactgc gccacctgcg acggagccat gtaccaggac cggcgtgtgg tggtggtggg
     4501 cggtggcaat tcggcggtag aggaagcgtt gttcctgacc cggttcgcct cccgggtgac
     4561 ga
//
LOCUS       DASVMB010000556         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_729283,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000556 DASVMB010000000
VERSION     DASVMB010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729283"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(932..2416)
                     /locus_tag="VF531_15585"
     CDS             complement(932..2416)
                     /locus_tag="VF531_15585"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX7715439.1"
                     /translation="MNDSAALWAQRQDSALATDFYELTMFYGFWKYGRHEQKSCFEYF
                     FRSLPPHNGFALFAGLEQVLDYVRNFTFNPSDLEYLGMQGFEPAFLEYLRDFKMAVDI
                     WAVPEGSVIFPNEPILRVEGPLGALQLLETFLLNALNYPTLVATKAARVCYAAQGDPV
                     MEFGLRRAQGPDGGLCGARAAIIGGCIGTSNVMAGKFYGAKVLGTHAHSWVMSFSDEA
                     EAFRAYAKAFPNNITLLVDTYDPLNSGIPNAVKVFSELRDAGFTGRAAIRLDSGDLAK
                     LSKAAWKMFQDAGFENPMIVASNELDEELIADLKRQGAKINSWGVGTHLITSSNFPAL
                     NGVYKMVAIQGPTGWEPKIKISGNTSKITDPGRKRIIRYFNDSGQPLADVLYLEEEQI
                     QQGRVQVFDRENLFKEIGFKAGHYQELSQPIVTAGKVLSPAVPLEALRQQATRNLAQF
                     PEEYRRLRYPQTYNVYLSPEAARVKQSLLEAAHFYRNETAAGRE"
     gene            complement(2409..2987)
                     /gene="pncA"
                     /locus_tag="VF531_15590"
     CDS             complement(2409..2987)
                     /gene="pncA"
                     /locus_tag="VF531_15590"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX7715440.1"
                     /translation="MSKPALIVVDVQNDFCPGGALAVQGGDEVVPVINELIPKFDMVV
                     YTRDWHPEHHVSFAEKPQFTDKSWPVHCVANSRGADLHPELTFTLNAMIVDKGTDPAQ
                     EAYSGFQGTDLAKTLHRLNIDTVYIAGLATDYCVKHTALDALQEGFKVKLVIDGCRGV
                     DVPPGSAAQTAQDLRQAGVDIITAKEVDSEHE"
BASE COUNT          840 a          901 c          916 g          852 t
ORIGIN      
        1 ggaacttctc acccactgtc ctggaattgc cgcagacggt agtggttcgt aacgaggatc
       61 tggccgcggg tcttgccacc ctgtttcatt atctggcgga ggagaagtag gggaagtacg
      121 aagccaaaag tctgaaagcg actcaccccc tggccccctc tctcccggca agcgggaaag
      181 agggggaagg cggatttaag gcagtgaaat agacatcaga ggattgtggt tacgcgcgca
      241 catgtgcgcg actctaacgt ttgccgttag tgagagcaag agtgagtcgc gggggacgga
      301 gccgactcac ctctcatgcg actcgtcacc taactttcgc ttcatttgga cgccgccaag
      361 cggaaagaga aaaaagcgga ttttctttgc tcttctcgcg cctcacgcct cgtgccttaa
      421 cttcatcgaa ccccatacat tctattgcct gttccctgtt cccttgcgtc tcatgagacg
      481 catatgcctt ttccccaata aaacgttacc aaagccgggt ttccagcgtc agtgctgcca
      541 cataaattat cagtgaccca taaccaatca acctcgaaaa ttaaaggagg tgcagacgtg
      601 ggtaccggac tcggtggctt ttttggtgga atcgatgagg aactggcttt tttgcttttc
      661 ctaatcctga ttctcttgat tttaggaatt aactaagaac ccccaaagat acctgttgca
      721 ttgaggcaat aggtatcttc cataccagga tgttgatccg gaatccgtaa aaaggaggcg
      781 tggaccaaat ggtagacaag aagggaggct tctttggcgg tagtttcttc tttatattct
      841 tcttcatcat cattattatg ttgtttatta attaaacatg atcaagatac cgccagtcgg
      901 cccgggaagt ggttactccc gggtcaactt tttattccct tccggccgcg gtctcgttcc
      961 ggtaaaagtg tgcggcctca aggagtgact gtttgacccg ggctgcttcc ggagaaagat
     1021 agacattgta ggtctgtgga taacgcagcc ggcggtattc ctcggggaac tgagccagat
     1081 tgcgggtggc ttgttgccga agcgcctcaa ggggcacagc cggtgacagg accttgcctg
     1141 ccgtgactat cggttgggag agttcttgat aatgcccggc cttgaatcca atctccttga
     1201 agagattctc cctgtcaaag acctggaccc ggccctgttg aatctgttct tcctccaggt
     1261 atagcacgtc ggctagaggc tggccggagt cgttgaaata acggataatc cgtttccggc
     1321 ccgggtctgt gatcttgctg gtattaccgg agatcttgat ctttggctcc caaccagttg
     1381 gaccttggat ggccaccatt ttatagacgc cgttcaatgc cggaaaatta ctggaagtga
     1441 tcagatgggt ccccacgccc caggagttga ttttggcgcc ttgccgtttc aaatcggcga
     1501 tgagttcctc atccagttcg ttggaggcca caatcatcgg attctcaaat cccgcgtcct
     1561 ggaacatctt ccaggcggcc ttggagagct tggccaggtc gcccgagtcc aggcggattg
     1621 cggcgcgacc ggtgaacccg gcatcccgta attcgctgaa gacctttacg gcgttgggaa
     1681 tcccgctgtt taggggatca tatgtatcca cgagcaacgt aatattattg ggaaaggcct
     1741 tggcataagc ccggaaagcc tcggcctcat cgctaaaact cattacccag ctgtgagcat
     1801 gagtacccag gactttggcg ccataaaact taccggccat tacattcgaa gtcccaatgc
     1861 atcccccgat gatggcggcg cgggcgccgc acaatccgcc gtccggcccc tgggcccggc
     1921 gcaggccgaa ctccatcacc ggatcgccct gagcggcgta acaaacccgg gcggccttgg
     1981 ttgccaccag ggtcgggtag tttaaggcgt ttagcagaaa agtttcgagc agttgcaagg
     2041 cgcccagggg accttccacc cgcaggatcg gttcgttggg aaaaatcacc gaaccctcgg
     2101 gaaccgccca gatatcgacg gccattttaa aatcgcgcag atattccagg aaggccggtt
     2161 caaacccctg catccccaga tattccaggt cactggggtt aaaagtgaag ttccggacat
     2221 aatccaaaac ttgttccagc ccggcgaaga gcgcaaagcc gttatgaggc ggcaagctcc
     2281 tgaaaaaata ctcgaaacag gatttctgct cgtgacggcc gtatttccaa aaaccgtaaa
     2341 acatggttaa ttcataaaaa tccgttgcca aagcggaatc ctgacgttgg gcccaaaggg
     2401 cagcgctatc attcatgttc ggaatcgacc tcttttgcag tgataatatc cactccggcc
     2461 tggcgtaaat cttgggcggt ctgggctgcg gagcccgggg ggacatccac gccgcggcag
     2521 ccgtcaatca ccaatttgac cttaaaaccc tcctgtaacg catccaaggc tgtatgcttt
     2581 acgcaataat cggtggctag gcctgcgatg tagaccgtat cgatatttaa gcgatgcagg
     2641 gttttggcca gatcggtccc ctgaaaaccg ctataggcct cctgagcggg atcggtccct
     2701 ttgtcgacga tcatcgcatt gagggtaaag gtcaattcgg gatgcaagtc cgcgccccgg
     2761 gagttggcca cgcaatgaac cggccatgat ttgtcggtga attgcggctt ttccgcgaaa
     2821 ctgacatgat gttcaggatg ccaatcgcgg gtgtagacca ccatatcaaa tttggggatc
     2881 agttcattaa ttactggaac aacctcatcg cctccctgaa ccgccaatgc acctccggga
     2941 caaaaatcgt tctgaacatc caccacaatc aaagcaggtt tactcaccta aaaacgcctc
     3001 ctttgaaaaa ggattcacga ttgataaatg ttattcgtgt aaatattcgc gcaagaccgg
     3061 agtatctcct gtaacaagaa accaggtttt ggatgaaaat tgaggtgagg cacctaaccc
     3121 ccggcccctt catttttgga cgctagcatc cgggacaggg aaggaggggt tattggagtg
     3181 acaaatgaga attcgacttt ccgtcatatg taattatatt cggagtttcg gcggctaaat
     3241 ttttatatgt ggtatcttat ctctccgaat tttctttgta gccgggacgt tcacgtcccg
     3301 gttgggggtg gagcaaatca aacattcctg tatgatcgta cggatgcaaa tcgccgaccg
     3361 gaggatgaat cctccggcta caagatggaa gggctttgcc ctatctctgg gatgaaatat
     3421 tatgtttttg gaccaacact ctggttagct gtagattcca acggggcagg attacagaac
     3481 gggttagcga atactattat ggaaaaagt
//
LOCUS       DASVMB010000557         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_658054,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000557 DASVMB010000000
VERSION     DASVMB010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658054"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1249
                     /locus_tag="VF531_15595"
     CDS             218..1249
                     /locus_tag="VF531_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7715441.1"
                     /translation="MKRKVTIRDVAKYAGVSVATVSNVINNIDKASDETREMVLKSIK
                     EIGYQPDFVARCLAKGKSNLIGVMLPITEKGDEPYLLLRDNPFFSEFISGIDFISRKR
                     GYDFLISGIESGQQCKEWVTKRNLDGIIMLGMLPRSFFNEMEEINLPVVLIDAYEEFA
                     TRFHRIMADDKNGGYLATKHLISLGHRHIAFVTGSVKQSGVNRERFEGYKKALQEAGI
                     TPDPGLIFEDHVTFEGGYQAGKRIMESGGEITAVVTVADVVAFGLINVFRDHGKTVPD
                     DYSVVGFDDIQLCKYVVPRLTTVRQNIFKKGVAAVEAIISDIELPSETSETKILSVEL
                     IQRESTKKL"
     gene            1395..1898
                     /locus_tag="VF531_15600"
     CDS             1395..1898
                     /locus_tag="VF531_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX7715442.1"
                     /translation="MKKFMLVALIVVGLIFSMITLAGADDTAASAVNLALNKPITESS
                     HTQYFFVKNANDGNINSYWEGAAGSYPDLLTVDFEAATKITSVVIKLNPKPIWEERTQ
                     TIEVLGSMDGATFTTFVAPVKYTFDPDTNQNIVTISVAANARYLRLSFTTNSEADAGQ
                     VAELEVY"
     gene            1904..>3429
                     /locus_tag="VF531_15605"
     CDS             1904..>3429
                     /locus_tag="VF531_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX7715443.1"
                     /translation="MKCQNGLSQKLKSTLIAITALIFLFDFSFVVFAGCDLVITDIAW
                     SPANPVAGDEDGTHAVMARLDDVRRMAEDNWDNKTYTKQLNIGGTPVTPAISAPTPSP
                     NQTGSQPISSPTPRPNLKNRGATVSYVEYEAESAVTSGTVLRADRTFHIIQSEASGRK
                     AVRLDTTGQYVEFTLTQPANSIVIRYCMPDAPGGGGIIAPISLYINGVHSEDIPLTSK
                     YAWVYGAYPWSNDPGQGNPHRFFDETRAIFKAGTLGAGTKVRLQKDAADTSAYYIIDL
                     IDMENVGPALTKPADFLSITDYGAVPDGSNDNTKEIKKCIAEAKSQGKGVWIPAGTFN
                     IAVRLKVDNVTIRGAGMWYSVLTGTGACFALNGNNCKFYDFALYGDTTIRDDSAPETG
                     FDGNAGTGSVIQNIWMEHLKCGIWVNGPTNGLVITGCRIRNTFADGINLCSGTCNTTV
                     SQCTLRNTGDDSIAIWSATWMGTDPCKNNVVRYNTIQCPWLANGLAVYGGADNKFEYN
                     V"
BASE COUNT          941 a          723 c          852 g          913 t
ORIGIN      
        1 gtaactcggt ggggaaagtt ctccgccgct tactgcggga gagggttgaa gatgaagttt
       61 gaattgtgaa gcgtgaaggg tgaaacagat atgtggtccg ccccaggaat ttgaatttaa
      121 ggcgaccttt gaatagagat tttatttccg gagaatttat tgacatccta aatcgttggt
      181 aatataatga agttaatcgg ttaacaaaga ggtttttatg aaaagaaaag tcacaattcg
      241 agatgttgca aaatatgccg gcgtttccgt agctacagtg tcgaatgtca tcaataacat
      301 tgataaagct tcggatgaaa cccgggaaat ggttttaaag tcgattaagg aaatcgggta
      361 ccaacctgat tttgtcgccc ggtgtcttgc caaaggcaag tccaatttga tcggagtcat
      421 gttgccgatc accgaaaaag gcgatgaacc ctatttactt ttgagagata atccgttttt
      481 tagcgaattc atcagcggta ttgattttat ttcccgtaag agaggttatg actttttgat
      541 ctccggtatt gaatccgggc agcaatgtaa ggaatgggtt accaaacgga atttagacgg
      601 gattattatg ttgggtatgt tgccccggag cttttttaac gaaatggaag agatcaattt
      661 gccggtggta ttgatcgatg cttacgagga gtttgcaacg cgatttcacc ggatcatggc
      721 cgatgataaa aacggtggtt atcttgcaac caagcattta atcagtttag gccatcgtca
      781 tattgcgttc gtcacgggaa gcgtgaaaca aagtggtgta aaccgcgagc gttttgaggg
      841 ttataaaaaa gccttgcaag aagcgggcat tactccggat cctggtttga ttttcgaaga
      901 tcatgttacc tttgagggtg gttatcaagc cggaaagaga atcatggagt ccggcggtga
      961 aataaccgcg gttgttacgg ttgctgatgt tgtcgccttt ggattgatca atgttttccg
     1021 ggatcatggc aagacagtcc ccgatgatta ttcggtggtt ggatttgatg atatacaatt
     1081 atgtaaatat gttgtgccgc gattgacgac tgtgcggcag aatatattta aaaaaggagt
     1141 tgcagcggtc gaagcaatca ttagcgacat tgaacttccg agtgaaacca gtgaaacgaa
     1201 aatcttatcg gtggagttga ttcaacggga atcaaccaaa aagctatagt ataggttggt
     1261 tttagactgt tgataaatca tttttggtag agggataatt ttttttgcaa aattagttaa
     1321 ccgtttaaca taaataatcg ttccgtgatt ttaaacaccg gagaaccaca aaatcatttg
     1381 aagggggtaa aaacatgaaa aagtttatgc tggttgcgtt gatcgttgtg gggttgattt
     1441 tctcaatgat tactttggcg ggagccgatg ataccgctgc gtctgctgtc aatcttgcgt
     1501 taaacaaacc aatcactgaa agtagtcata cgcagtattt cttcgtaaaa aatgccaatg
     1561 acggcaatat caattcctat tgggaaggcg ctgccggctc atacccggat ctgcttaccg
     1621 ttgatttcga ggcggccact aaaattacat ccgtcgtcat caaactgaat cccaaaccga
     1681 tctgggaaga acggacccaa accatagaag tccttggcag tatggatggt gctacgttta
     1741 ctacattcgt tgctcccgta aaatatacct ttgatcccga taccaatcaa aatatcgtta
     1801 cgatatcagt cgcggccaat gccagatatc tgcgtttgag tttcaccacc aacagtgaag
     1861 ccgatgcagg ccaagttgcc gagttggaag tgtactaacc ctaatgaaat gccaaaatgg
     1921 gctgtctcaa aagctgaaaa gtactttgat tgcaataacc gcgctgatct ttttgtttga
     1981 cttttcattc gtcgtttttg ccgggtgtga tctggtcata accgacattg cttggtctcc
     2041 ggcgaatccg gtggccggtg acgaagacgg aacccatgcc gtcatggcta ggttggatga
     2101 tgtcaggcgg atggctgaag ataattggga taataaaacc tataccaaac agttgaatat
     2161 tggagggacc ccagtgactc cggcgatttc agctccaaca ccaagtccga accaaacagg
     2221 ttcgcaacca atatcttcac ctactcccag gcccaatctg aaaaatcggg gagctaccgt
     2281 atcttacgtg gaatatgaag ctgaaagtgc tgtcaccagc ggtaccgttc ttagggccga
     2341 tcgtaccttc cacatcatcc agtcggaagc atcaggcaga aaagcggtaa gattggacac
     2401 caccggccaa tatgtggaat tcacgcttac tcaacccgcc aactcgattg tgattcgtta
     2461 ctgtatgcct gacgctcccg gcggtggcgg cattatagct ccaattagtt tgtatatcaa
     2521 tggagttcac tccgaagata ttcccttaac ttccaagtat gcctgggtat atggggcata
     2581 tccctggtcc aacgacccgg gtcagggcaa tccccaccgt ttcttcgatg aaacccgggc
     2641 aattttcaaa gccgggacgt taggcgccgg cactaaagtt agattgcaga aggatgccgc
     2701 agatacctcg gcttactata tcattgactt gatcgatatg gaaaacgtag ggccggcatt
     2761 aaccaaacct gccgatttcc tttccatcac ggattacggt gccgtcccgg acggcagtaa
     2821 tgacaatacg aaagagatca aaaaatgtat tgctgaagcc aaatctcaag gcaagggggt
     2881 ttggatcccg gcgggaacct ttaatatagc agtccgcctc aaggttgaca atgtaaccat
     2941 tcgcggcgcc ggaatgtggt attcggtgct tacgggaacg ggtgcctgct ttgcattgaa
     3001 cggcaacaac tgcaaatttt atgattttgc cttatacggc gataccacca tccgggatga
     3061 tagcgccccc gaaaccggat ttgacggcaa tgccggaacc ggttcggtga tccaaaatat
     3121 ctggatggag catttgaaat gcggtatttg ggtcaatggt ccgaccaatg gactcgtcat
     3181 taccgggtgc aggattcgga atacctttgc cgatgggatc aatctttgca gcggtacctg
     3241 taacacgacg gtctcccagt gcacgctccg gaacaccgga gatgattcaa tcgccatatg
     3301 gtccgccacc tggatgggta cagacccttg taaaaataat gtcgtacgtt ataataccat
     3361 ccaatgccca tggttggcca acggcctggc cgtctacggc ggggcggata ataaatttga
     3421 gtacaatgt
//
LOCUS       DASVMB010000558         4976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_658063,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000558 DASVMB010000000
VERSION     DASVMB010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658063"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF531_15610"
     CDS             complement(<1..392)
                     /locus_tag="VF531_15610"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX7715444.1"
                     /translation="MAEDTSTNTNANPTPKAAAKTATAGAGNKFTMYIIIGLIVLLIT
                     VMIMATVLITTFSNLKAPQTIVKTVPSAPVQSEEVGLWVPLGEMIINLYSDDPATAHY
                     LKINITLEVAKDEKIKEEITDRVPEIKD"
     gene            complement(545..757)
                     /locus_tag="VF531_15615"
     CDS             complement(545..757)
                     /locus_tag="VF531_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEX7715445.1"
                     /translation="MIEVRRLNNEELFINPHLIEIIESTPDTVITLTTGKKLVVKDPV
                     ADIVERIIRYRRMIAGPTITESGETR"
     gene            complement(1000..3261)
                     /locus_tag="VF531_15620"
     CDS             complement(1000..3261)
                     /locus_tag="VF531_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX7715446.1"
                     /translation="MMRSMYSGVAGLLNHQIRMDAIGNNIANVNTVGYKSSRVTFQDT
                     LNQTLRGASAPNGNRGGINPVQVGLGMNLASIDILETQGNLQNTGRTTDVALQGDGFF
                     IVNDGNRQYYTRAGNFNLERDGRLVNASNGMIVQGWVADNLGNINANAPVTDLRLPLG
                     QIISPIATTHMELGGNLASNTNINVEYAELALTDPDKNPITVKFKLIPTGFNQWNYTA
                     EVDEQYGSITGGTPSGTLTLNENGVVTGTGGFFEVTPKNGTEIRIEPPRLGQLNGGYF
                     AADQTGTNQDIQLGALTGAGPWTFDDFTAPADFGPLQDANGNPLNITYNITALGNDRY
                     QWQITSGANTQLVSGGIGTFQWTSTRGVYNAEPSTATVIKSTLAGGKGNVTINAPSEQ
                     TGNPFFSVNELAPVTLPATSTPVGAIPGTLSYNVFNASGELLTRLHFASTDGADPRNY
                     ILTVDNGTVLNGADGVLNWDGTNIAGFTPTGGNAVIKLNNGVEITIPTGAPANPSFTT
                     LLAVGSKNEQVEATIPDPVTTMTKVYDSLGKEHMITTTSTKVGDNRWTWETMDEYNNP
                     IGNGDLYFDSQGNFVSYTGGPIVFTPFGAQTVSITPDFSDLRQCAGSLDADTKMAISE
                     FNSPYQDGYPMGQLDSINIDKAGRIIGVFSNGMNQNLGQMAVAYFTNPGGLTRSSDTL
                     FEESSNSGTAQIGQAGQGGRGQVTPGAIEMSNVDLSQEFTDMITTERGFQANSRIITT
                     SDEMLQELVNLKR"
     gene            complement(3397..3786)
                     /locus_tag="VF531_15625"
     CDS             complement(3397..3786)
                     /locus_tag="VF531_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HEX7715447.1"
                     /translation="MSEKIFISPQIGPATTGVGPQPVKVPVQGASFESLFQKELEGQT
                     EVKFSAHALKRLESRQIALNDKDMNLIKDAINRAEAKGAKESLILLDNLALVVSIKNR
                     TVITAVDEGSMKDNVFTNIDSAVIARQ"
     gene            complement(3779..4639)
                     /locus_tag="VF531_15630"
     CDS             complement(3779..4639)
                     /locus_tag="VF531_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX7715448.1"
                     /translation="MISGTDATTQATGLSSGSDTQTRTPKSELGKDDFLNLLVTQLRY
                     QDPLKPMEDKEFIAQMAQFTSLEQMQNLNKAMSSIQAAGMIGRTVKAEINGEDGTELV
                     YGTVISSKEISGEIYLNLDNGRQIKAKDATTTFSGEGLWKEAESMVEKNVYVRDGVDA
                     DGNTDNVYVMYVAGIKQIVDTVGNSTIKLLTSNSYKTVTGNLTDAQALVGKKVYIKQY
                     DEEGKESDVLLQVKVTGAEVENNTVKLKYGSGDSEQTFEFKDIMNINDAIELKDIWNV
                     VPDAEDVSNE"
     gene            complement(4677..>4976)
                     /locus_tag="VF531_15635"
     CDS             complement(4677..>4976)
                     /locus_tag="VF531_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX7715449.1"
                     /translation="TVTAKFTAESQQVKQILESNMDDLRRHLQENGIQVDSLMVSVGN
                     SGTGNEQFARGSYNQDFRNDSGNFGYSQPADQDEVESGREETRRPVRDTVIDLIA"
BASE COUNT         1074 a         1306 c         1206 g         1390 t
ORIGIN      
        1 aagtctttaa tctcgggaac tctgtccgta atctcttctt taatcttttc atccttggct
       61 acttccagtg tgatattaat cttcagatag tgagcggtgg ccgggtcatc cgaataaaga
      121 ttgatgatca tttcccctaa aggtacccaa aggcccactt cctccgattg aacgggagca
      181 gatggaacgg ttttaacaat ggtctgaggt gccttgagat tactgaaagt cgtaatcaag
      241 accgtagcca tgatcattac cgtaattagc aaaacaatca atccgatgat gatatacatt
      301 gtaaacttat tgcctgctcc ggcagtcgct gtttttgcag ctgctttcgg tgtggggtta
      361 gcattagtat tagtgctagt atcttccgcc acgagtcaac cctccctgga acaacatgat
      421 agatcccatc atcatgttat attttttggt acataaacat cctggattta tctgtatagt
      481 ttttatgata ctatgagttg caaaatatat caataaagcg gattcatttc gagaatgaat
      541 gagtttaccg ggtttcaccg gattcggtaa tggttggccc ggcgatcatc cgtcggtaac
      601 ggatgatgcg ttccacgatg tcggctaccg gatctttaac caccagtttt ttcccggttg
      661 tcaaggtaat caccgtgtct ggagtggatt caataatctc tatcaaatga ggattaatga
      721 acagttcctc attatttagg cggcgaactt cgatcatggc tttgcctccc ggttattagt
      781 gaaatgaaag ctggcttcgc atgcaaatcc cggtcgggtt tgcgccgact accctatccc
      841 tgaaagggaa cctgccaagc ctttgcaggt ctttggtttt ctgccaaacc cgtccgatcc
      901 cctctcaggg atagggtgga tagatccgga ttcgggccca tcaccctaac cctcatgtct
      961 tttacctatc cctgttgcct gttgcctgtt gcctatgtct taccgtttca gattcaccaa
     1021 ttcctgcagc atctcgtcgg aagtggtaat gatcctcgag ttggcctgga agccgcgttc
     1081 cgtagtgatc atatccgtga actcctgtga gagatcgaca ttggacatct caatcgcacc
     1141 cggagtcacc tggccccggc cgccttgacc ggcctggccg atttgagcgg ttccggagtt
     1201 gctgctttct tcaaacagtg tatcgctcga gcgggtcaaa cctcccggat tcgtgaaata
     1261 ggcgacggcc atctgtccta aattttgatt catcccgttg gagaagacac cgatgatccg
     1321 gccggcctta tcgatgttga tcgagtcgag ctggcccatg ggatacccat cctgatacgg
     1381 ggagttaaac tcggaaatag ccatcttcgt gtcggcatcg agcgatccgg cgcattgccg
     1441 taagtcgctg aaatccggag tgatgctcac cgtctgggcg ccgaacggtg taaagacgat
     1501 gggaccgccg gtatatgaaa caaaattgcc ctggctgtca aagtagagat cgccgttgcc
     1561 gatgggattg ttatactcat ccatggtctc ccatgtccag cgattatccc ccaccttggt
     1621 ggaggtggtg gtaatcatgt gttccttgcc cagggagtcg tataccttgg tcatggtcgt
     1681 caccggatcc gggatggtgg cctccacttg ttcgtttttg ctcccaactg caagaagagt
     1741 agtgaaactg gggtttgcag gtgcacccgt cgggatagta atctcgactc cattgttgag
     1801 cttaatcacc gcattaccgc ccgtcggggt gaaacctgcg atgtttgtcc catcccaatt
     1861 tagtacacca tctgcaccgt tcaacactgt accattatca actgttagga tatagttacg
     1921 aggatctgcc ccatcagtgc tcgcaaaatg aagtcttgtc aaaagttcac cactggcgtt
     1981 gaatacgttg tagctgagcg taccgggaat agctccaact ggggttgaag tggcgggtaa
     2041 cgtcaccggc gcaagttcgt tcacagagaa gaacggattc ccagtctgct cggatggcgc
     2101 gttgatggta acgtttcctt ttcctccggc cagcgtcgat ttaatcaccg tcgcggtgct
     2161 gggttccgca ttatagacgc cgcgggtcga tgtccactgg aaggttccga tgcctcccga
     2221 tactagctgg gtgttggcgc cagaggtaat ctgccattga tatctgtcgt ttcctaaagc
     2281 ggtgatatta taagtaatat tcaacgggtt gccattggcg tcctgtaacg gtccaaaatc
     2341 tgccggtgcc gtaaagtcgt caaaggtcca gggtcccgct ccggtgagcg ctcctaattg
     2401 aatatcttga ttcgttccgg tctgatccgc ggcgaagtaa ccgccattca actgtcctag
     2461 ccgcgggggt tcgatccgga tctcggtgcc gtttttcggg gttacctcaa agaaaccacc
     2521 ggtgcctgta acaaccccgt tctcattgag ggttagcgtt ccggaaggtg taccccccgt
     2581 aatactccca tactgctcat caacttctgc ggtgtaattc cactgattga accctgttgg
     2641 tatcaattta aatttgaccg taatcgggtt tttatcgggg tcggtcagtg ccagctccgc
     2701 atattcaaca ttgatattgg tgttggaggc caggttgcct ccgagttcca tatgagtagt
     2761 ggcaatgggc gaaatgatct gcccaagcgg caaccggaga tcagtcaccg gagcattggc
     2821 attaatattg cccaagttat cggccaccca tccttggacg atcataccgt tggaagcgtt
     2881 caccaagcgg ccgtcccgct ccaggttgaa attgccggcc cgggtataat actgacggtt
     2941 tccgtcattg acgataaaaa agccgtcacc ctggagggct acatccgtgg ttctcccggt
     3001 attctggagg tttccctggg tttccaggat atcgatactt gccaggttca ttcccaaacc
     3061 gacctgcact gggttgattc cgccgcggtt gccgttggga gccgaagcgc cgcggagtgt
     3121 ctggttgagc gtatcctgaa aggtgacccg gctgctttta taaccgacgg tgtttacgtt
     3181 ggcgatatta ttaccgatgg catccatccg gatctgatga tttaacagac cggcgacgcc
     3241 ggagtacatg gaacgcatca taataaatcg acctccttta gttaatggta ccccgcctcc
     3301 gtcattcggc gggtctgagg cttcctcagt ttttaagagg tccggcctca ttctttcacg
     3361 cgccagtctt ggtgaaatca ttgtttaacg tacaatttat tggcgcgcaa tcactgcgct
     3421 gtcgatgttg gtaaagacgt tatccttcat actgccttca tccactgcgg taatgactgt
     3481 ccggttcttg atactgacca ccagcgccag gttgtccaac aaaatcaggg attccttggc
     3541 gcctttggcc tcggcccggt tgatcgcatc tttaatgaga ttcatatctt tgtcgtttaa
     3601 agcgatctgc ctgctttcaa gcctttttaa cgcgtgagcc gagaacttaa cctcggtttg
     3661 gccctcaagc tccttctgga acaggctttc aaagcttgcg ccttgaaccg ggacttttac
     3721 cggttgcggc ccgactccag tggtcgctgg accaatctga ggcgagatga agattttttc
     3781 actcattgct gacatcctct gcatcgggga ctacattcca gatatccttc agctcgatgg
     3841 catcgttgat attcatgatg tccttaaact caaaggtctg ttcggagtca ccgctcccgt
     3901 acttcaattt caccgtgttg ttttccacct cggcaccggt aacctttacc tgaagcagga
     3961 catcgctctc cttgccttct tcatcgtatt gcttgatata gactttcttg ccgaccagag
     4021 cctgtgcgtc ggtcagattg ccggtaacag ttttgtaaga attggaagtc aacaatttga
     4081 tggtgctgtt tccgacagtg tcgacaatct gtttgatacc cgccacatac atcacataga
     4141 cgttatcggt attaccatcg gcatcaacgc cgtcacgaac gtatacgttc ttctccacca
     4201 tggattcggc ttctttccag agaccctcgc cgctgaaagt agtcgtggca tcctttgcct
     4261 tgatttggcg gccattgtcc agattcagat agatttcgcc gctaatctcc ttggatgaaa
     4321 ttaccgtacc gtaaaccagt tccgtgccgt cctccccgtt aatctcggct ttgaccgtcc
     4381 ggccaatcat tcccgccgct tgaattgacg acatggcctt attcaagttc tgcatctgct
     4441 caagcgaggt gaactgagcc atctgagcga tgaattcctt atcttccatc ggttttaaag
     4501 gatcctggta acgcagctgg gtgacgagga gattgagaaa atcatctttg cccagttccg
     4561 atttgggtgt gcgggtttga gtatccgacc ccgatgaaag acctgttgct tgagtcgttg
     4621 catccgttcc gcttatcacc ttgtatcacc tctttccgtt tatacaatct ttcagcttac
     4681 gcaatcaaat caatgactgt atcccgaacc gggcggcgcg tctcctcgcg acccgattcc
     4741 acttcatcct gatccgcagg ttggctgtaa ccgaagtttc cggaatcgtt acggaaatct
     4801 tggttatagg atccccgggc aaactgttca ttaccggtgc ccgaatttcc caccgagacc
     4861 atcaacgaat ccacctggat accgttctcc tgcaagtgcc gccgtagatc atccatattg
     4921 ctctccagga tctgcttgac ctgctgcgac tcggccgtaa actttgcggt aaccgt
//
LOCUS       DASVMB010000559         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_729833,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000559 DASVMB010000000
VERSION     DASVMB010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729833"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VF531_15640"
     CDS             complement(<1..426)
                     /locus_tag="VF531_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7715450.1"
                     /translation="MSTTTSNRRRPFFFGLIIPLGLAVLILAREVIWPFVAAFFLAFA
                     INPVVDFFQRKGARRDWAILTVYLVLTVLVILCFQLLIPLLVNDITEILQRLPVIFKE
                     LETGGDRLIRSLDSWPLPFNPRALITDLGRRGESILRQTL"
     gene            complement(619..864)
                     /locus_tag="VF531_15645"
     CDS             complement(619..864)
                     /locus_tag="VF531_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715451.1"
                     /translation="MNRLIRGAITGSLIGAGIGVTMLWMRRRNQSFMKSAMVQPHEMR
                     GRTRGTLHMVKNNAMRWTSAVKSGTEAFSRRLAHRIS"
     gene            complement(987..2078)
                     /gene="mnmA"
                     /locus_tag="VF531_15650"
     CDS             complement(987..2078)
                     /gene="mnmA"
                     /locus_tag="VF531_15650"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7715452.1"
                     /translation="MMGARVLMAMSGGVDSSVAAALLLEQGYEVIGVTMQLWSEALPY
                     GVEVEGGCCSLGAVEDARAVAGKLGIPYYVLNFHDQFQKQVIDYFANEYLAGRTPNPC
                     IVCNNLLKFGGLLDKARALGADYVATGHYARTGPDPESGRYLLMKGQDSSKDQSYVLY
                     GLTQEQLARTLFPLGNFTKPAIRQKAADLGLKVATKPDSQEICFVPDQDYSRFIAEYR
                     PGEMRPGKIRDTDGTVLGEHSGVANFTIGQRKGLGIASGTPLYVTAIRPETNEVVVGT
                     AEEVMADGLFATGLNWIAIPYLKGPLPAEVKIRYSARPVPALLEPDNSGRVAVRFERS
                     QRAITPGQAAVFYNGDQVIGGGTIEESFS"
     gene            complement(2075..2446)
                     /gene="nifU"
                     /locus_tag="VF531_15655"
     CDS             complement(2075..2446)
                     /gene="nifU"
                     /locus_tag="VF531_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HEX7715453.1"
                     /translation="MYTEKVMDHFQNPRNVGEITDASGVGQVGNPVCGDIMKIYLKIE
                     GERILDVKFKTFGCGAAIATSSMVTEMVKGKTIDEALEITNQAVAEALDGLPPQKMHC
                     SNLAADALHKAIEDYRMRQAG"
     gene            complement(2428..3627)
                     /gene="nifS"
                     /locus_tag="VF531_15660"
     CDS             complement(2428..3627)
                     /gene="nifS"
                     /locus_tag="VF531_15660"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX7715454.1"
                     /translation="MKRIYMDHAATTPVDPVVLEAMTPYFTTNFGNPSSIYWYGRENR
                     KVLDDARATVAAEIGAENPSEIIFTGGGSESDNLAIKGVAEAYRDKGNHIITSAIEHH
                     AVLNTCEYLEKHGYKVTYLPVDGDGLVSPEAVANSLTDSTILVSIMMANNEVGTIEPI
                     AEIGKLLRERKVLFHTDAVQAVGSIPVNVRDLSVDLLSMAAHKFYGPKGVGALYVRKG
                     VKLQPVILGGAQERNRRAGTENVPGIVGLAKALQLANANMNETTPRITELRDYLIDNV
                     LNRFDHVRLNGHRTKRLPGNTNFSFEFIEGESMLLNLDFKGIAASSGSACTSGSLEPS
                     HVLLGMGICHAVAHGSLRLTLGKENTREEVDYLLGVLPEIVDKLRAMSPLYEAERKEC
                     ATCTQKR"
     gene            complement(3642..4091)
                     /locus_tag="VF531_15665"
     CDS             complement(3642..4091)
                     /locus_tag="VF531_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7715455.1"
                     /translation="MRISTKGEYGIRAMLELALHYREGPMALGKIAERQAISEPYLEQ
                     LVASLRKAGLLTSVRGALGGYELTRDPGEIAIGEIIRALEGPIAPMECVREETEGLGC
                     ENSERCATRLLWRKLQASINEVLDTTTLGDLVADNKRLNQDEVMYYI"
BASE COUNT         1023 a         1251 c         1025 g         1055 t
ORIGIN      
        1 tagcgtctga cgcagaatcg attcgccccg acggcccaaa tcggtgatga gtgcacgtgg
       61 attgaatggc aagggccagg agtcaaggga tcggatcaac cggtcgccac cagtctccaa
      121 ttccttgaaa atcaccggca aacgctgcaa aatctcggtg atatcattca ccaagagcgg
      181 gatgagtagc tgaaagcaga gaatgactaa aaccgtcaag accagataga cagtaaggat
      241 cgcccaatcg cggcgcgccc ccttcctctg aaagaaatcg acgaccggat tgatggcaaa
      301 ggcaagaaaa aaggcggcca cgaaaggcca gatcacctcg cgggccaaga ttaaaacggc
      361 caggccaagc ggaatgatta gtccgaaaaa aaaaggtctg cgacgattac ttgtcgtggt
      421 gctaattcac cccgctcccg tagaacatat cctgtcacga gtcctccaaa ccccgataaa
      481 gggattacaa acggccggct cgaaggccgg ccgaacgatt ccatcatacg attatttgcg
      541 cagcgcgcat gggatggatg cgggttgatt gaaactcgat ttcaccgtcc ggtacatcat
      601 cccctgctga aaaccttctc aagaaatacg gtgggccaaa cggcgggaaa aagcctcggt
      661 accgcttttg acagcagatg tccagcgcat ggcgttattt ttcaccatat gcagggtgcc
      721 tcgagttctc ccccgcatct catgcggttg taccatcgcc gatttcatga aactctgatt
      781 acgccgtcgc atccataaca ttgtaacacc gattccggcc ccgatcaaac taccggtaat
      841 ggctccccgg atcaacctgt tcattatctc acccctttca tggagggctc ctctttttag
      901 aatgattcct ccaaaacccg gtttatagga gatagtatct cccaatcagg caagattcat
      961 gaacggttta cgaaacatat gaaatttcaa gaaaatgact cttctatggt tccgccgccg
     1021 ataacctgat cgccgttgta aaagactgct gcctgccccg gggtgattgc ccgctgcgac
     1081 cgttcaaacc gcaccgccac ccgtccgctg ttatccggct ccagtaaggc cggaacaggc
     1141 cgcgcgctgt aacggatctt gacttccgcc ggaagcggtc cttttaaata aggaatggca
     1201 atccagttca gtccggttgc aaaaagccca tcagccatta cttcctcagc ggtgccgact
     1261 acgacttcat tggtctcggg ccggatggcg gtgacataaa ggggtgtacc cgaggcaatc
     1321 cccaaccctt tacgttggcc aatggtgaaa ttggcaaccc cgctgtgctc acccagtacg
     1381 gttccgtcgg tatcacgtat cttgccgggc cgcatctctc cgggccggta ctccgcgata
     1441 aatcgggagt agtcttgatc cggaacaaag cagatctcct gactatccgg tttggtggcc
     1501 acctttaaac ctaaatccgc cgctttttga cgaattgcag gcttggtgaa attcccaagg
     1561 ggaaagaggg tcctggccaa ctgttcctgg gtcaggccgt ataacacgta actctggtcc
     1621 ttggaggaat cttggccttt cattaagaga taacgcccgc tttccgggtc ggggccggtc
     1681 cgggcgtaat gcccggtagc aacatagtcg gcgcccaagg cgcgggcctt gtcaagcagt
     1741 ccaccgaatt tcagcagatt gttgcagaca atacaagggt tgggggtccg tcccgccaga
     1801 tactcattgg cgaagtaatc gataacctgt ttttgaaact gatcgtgaaa atttaagaca
     1861 tagtatggga ttcccaactt gccggctacc gcccgggcat cctctaccgc ccccaatgag
     1921 cagcagccgc cttcgacttc tacgccgtag ggaagagcct ccgaccagag ctgcattgta
     1981 accccgatga cttcataacc ctgttccagc agtaatgcgg cggctaccga actgtcgacg
     2041 ccgccgctca ttgccatcag aaccctggcc cccatcatcc ggcctgccgc atccgataat
     2101 cttcaatcgc cttatgcaag gcgtcggccg ccaggttgga gcaatgcatc ttttggggag
     2161 gcagcccgtc cagggcctca gccacagcct ggttggtgat ctccagagcc tcatcgatgg
     2221 ttttgccttt aaccatctcc gtcaccatac tactggtggc gatggctgcg ccgcacccaa
     2281 aggtcttgaa ctttacatcc aagatccgct ccccttcgat cttgagatag atcttcatga
     2341 tatctccgca aaccggatta ccaacctgcc cgacaccgct agcatccgta atctccccca
     2401 cgttacgcgg gttctgaaaa tgatccatta ccttttctgt gtacatgtcg cacactcctt
     2461 tctttccgct tcatataaag gtgacatcgc ccgaagttta tcgacgatct ccggtaagac
     2521 ccccaaaagg taatctacct cttccctggt gttctccttc cccaaagtca gcctcagtga
     2581 gccgtgggcc accgcgtggc agatccccat tcccaaaagc acgtgggatg gttccagaga
     2641 cccggaggtg catgccgaac cgcttgaagc ggcgatccct ttaaaatcca ggtttagcaa
     2701 catggactcg ccttcgataa attcaaagct gaaattggtg tttcccggca gccgcttggt
     2761 acgatgcccg ttcaatctca catggtcaaa acggttcaat acattatcga tcaaataatc
     2821 ccgtaattcg gtgattcgcg gtgtggtttc gttcatattg gcgttggcca gttgtaacgc
     2881 cttggcgagg ccgacgatcc cgggtacatt ctcggtaccg gcgcggcgat tgcgttcttg
     2941 ggctcctcca agaatcaccg gttgcagttt gactcctttt cgaacataaa gtgctccgac
     3001 ccccttaggc ccgtaaaact tgtgggctgc catagacaga aggtcaactg aaagatcccg
     3061 aacgttcacg gggatggagc cgactgcctg aaccgcatcg gtatggaaga gcaccttccg
     3121 ttcacgcaag agtttaccga tttcggcgat gggttcaatc gtacctacct cattgttggc
     3181 catcatgatc gataccagga tggttgaatc agtcagacta ttcgccaccg cttccgggct
     3241 tactaaacca tccccgtcta ccggaagata ggttaccttg taaccgtgtt tttcaagata
     3301 ctcacaggtg ttcaaaaccg catgatgttc gatggccgag gtgatgatgt gattgccttt
     3361 atccctatag gcttcggcta cccccttgat cgccagatta tcgctttcgg atccgccgcc
     3421 ggtgaaaatg atctcactgg ggttctcggc tccgatctcc gcagccactg tcgcccgggc
     3481 atcgtccaac accttgcgat tttcccggcc ataccagtat atactcgatg gattgccgaa
     3541 attggttgta aagtaaggag tcattgcctc caaaaccact ggatccaccg gggtggtggc
     3601 cgcatgatcc atataaattc gtttcatcaa taaacacctc ttcagatata atacatgact
     3661 tcatcctgat ttaatcgttt attatcggcg acaaggtcac ccagggttgt ggtgtcgagc
     3721 acctcattga tgctggcctg caatttgcgc cataacaacc gggtggcaca acgctcggag
     3781 ttttcacatc ccaaaccttc agtttcctcg cgaacgcact ccatgggagc gatgggccct
     3841 tcgagtgccc ggataatctc accaatcgcg atctctcccg gatcacgggt cagttcatac
     3901 cctcccaggg ctccccggac gcttgttaaa agtcctgctt tacgcaaact tgctaccagt
     3961 tgctcgaggt acggttcgga gatagcctgc cgctcggcga tctttcccaa agccatcggc
     4021 ccctcccggt aatgcagcgc cagctccagc atggccctga ttccatactc ccctttggtg
     4081 gaaatacgca acccgttccc ctcactttca aaaccgagta ttttcttaaa atttaattac
     4141 gagtaattta ctatttatta atccctacct atttactagg ttttacagcc tcatattatc
     4201 ataccctgga tagactgtca atatctgatg ccagtggaga gcatttccct ttggcccatg
     4261 aaattcaggg ttattatgcc aatatgaacg atcatttagt aacgcgactt tgggacattc
     4321 gcgttactct ttttattttc atttcacact tttc
//
LOCUS       DASVMB010000560         6819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_215115,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000560 DASVMB010000000
VERSION     DASVMB010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215115"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..439
                     /locus_tag="VF531_15670"
     CDS             269..439
                     /locus_tag="VF531_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715456.1"
                     /translation="MMRIGDLLIQGGFISEIQLERALELQRTQPEKKIGELLVELGYV
                     SSGDIIDTLKKQ"
     gene            complement(545..3289)
                     /gene="adhE"
                     /locus_tag="VF531_15675"
                     /gene_synonym="adhC"
     CDS             complement(545..3289)
                     /gene="adhE"
                     /locus_tag="VF531_15675"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEX7715457.1"
                     /translation="MGKKDTRNEQHDTVVKEGLPVQASPVKEVEGKPSVSEKTAATER
                     ISTVIDELVKQAEIAKNRFMEYNQEQIDAIVKAMALAGIDKHMELARMAATETGMGIY
                     EDKIAKNLFATEEVYHNIKNEKTVGIIEENEEEGFMKVAEPIGIIAGVTPVTNPTSTT
                     MFKCLISIKTRNPIIFSFHPKAIRASIAAAKVMLDAAVAAGAPENCIGWIAEPSIQAT
                     NLLMQHRGISMILATGGTNMVQAAYSCGKPALGVGPGNVPCYIEQTANLRRAVSDLIL
                     SKTFDNGVICASEQAVVIDRAVADRVKDLMREYGCYFLKPDEIGKLAAVAIDPERDSM
                     SPAVVGQPAAKIAAMAGFEVPEGTKILVAELEGVGPEYPLSREKLSPILACYTVADYH
                     EGIKLCEKITEFGGLGHSAVIHSGSQEVIDEFARRLRTGRLLVNSPSSHGAIGDLYNT
                     NTPSLTLGCGSMGHNSTTDNVSVRNLINIKRVAIRRDRMKWFKIPPKIYFEYGSLQYL
                     SKIKGKRAFIVTDPFMVKLGFPEKVTYQLDKIGFDYRIFSEVEPDPSVDTVMNGCREL
                     NAYQPDLIIALGGGSAIDAAKGMWLFYEHPDLEFETLRLKFADIRKRAFKFPDLGKKA
                     TFVAIPTTSGTGSEVTAFAVITDKKRNIKYPLADYELTPDIAIIDPDLVMTVPQKVTA
                     DTGMDVLTHAIEAYVSVMASDYTDALAEKVIKMVFEFLPRAYQNGQDRVAREKMHNAS
                     CMAGMAFTNAFLGVNHGMAHTLGGKFHIPHGRANAILLPYVIGYNAKKPSKFMAFPQY
                     EYPIAGERYAEIARLLGLPADTPEQGVASLIKAVKELMVKVNIPLTLAEAGVDREAFE
                     REVREMADLAFNDQTTGTNPRMPLVAEIEEVYREAYGAPQSKPSQSKVTDKTPSLAGV
                     "
     gene            complement(3340..4011)
                     /locus_tag="VF531_15680"
     CDS             complement(3340..4011)
                     /locus_tag="VF531_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX7715458.1"
                     /translation="MRKMRSERKAAVIPEAVMKRLPVYQRYLSEMLHQEYERISSQEL
                     AAKIGITASQLRQDLSYFGSFGQQGYGYHTGELLSQVNKILGLDRTKQVVLVGAGHLG
                     QALLSYTNFTERGFIYKAAFDIQPAPGGRVAGIPVLPVAALSEYLAETPVEIGIITTP
                     AGVAQEIATTLVRGGVKGIWNFAPVALNVPSTVVVEHVHISESLLVLSFKLEQERRWA
                     GKNPE"
     gene            complement(4165..4596)
                     /locus_tag="VF531_15685"
     CDS             complement(4165..4596)
                     /locus_tag="VF531_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7715459.1"
                     /translation="MGNEFVAVSAELINDKVQFQGKTRTNQPIICDYAPPLGDGQGYT
                     GLELFLVSLADCSGSTVVSLLRKMRKDVSGFRVNAQGVRREEHPTYFEKITLEFILDS
                     RDAAEADLQKAIRLSEEKFCPVWAMIKNNVEVATRFKIVAS"
     gene            complement(4817..5896)
                     /locus_tag="VF531_15690"
     CDS             complement(4817..5896)
                     /locus_tag="VF531_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715460.1"
                     /translation="MATVRFENVSKSFGETSVINDISFEIKDGELITLLGPSGCGKST
                     TLKMIAGLLNPSRGKIFFDSRRIDTVPSNQRNIGLVFQNYALFPHMTVFQNVAFGLEM
                     KKTKPDVIARRVDEVLRLVHMEEYQKRKPAELSGGQQQRVAIARALAFDPDVLLLDEP
                     LSNLDAKLRESVRLELRAIQKETGKTTIFVTHDQIEALTMSDRIFLMNSGKIEQEGSP
                     LEVYNRPRSIFSADFIGSNNFIDGLVKEVTANEFVLQFANQVVRLPKTYVVFTEGIKA
                     GIQSRLCIRPESIKWCSSNTGTSGVNFRGKVLEKIFMGGYVFVYLNIEDAILKFQVDQ
                     NEAAEIEIGQNTAVSVNECILLPRS"
     gene            complement(6006..>6819)
                     /locus_tag="VF531_15695"
     CDS             complement(6006..>6819)
                     /locus_tag="VF531_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7715461.1"
                     /translation="RKTLSKAGFYSYLGMMVIWFAFPLFLIVYASFQGTLDISLIPKS
                     LSLKAYYSIPRSYWDSFIFSLWLAVLAAIVSVMVAAPASWAMVRGRLPGKNLLSNIVL
                     LPILIPKIVTAIALLRFFLPIHLTNNFLGLLIALVGANGLPLAYRYTLAVAEGVNATY
                     EEAAMTLGAGKMTVFTRIVLPLMGPGIIVSALFVFMSNLMSFLIIFFIAGPMSSPISI
                     RLFSDIVERGALPHSIAMAAILIFVAVLFYVLISVFLGPAYLSGVVFAKKDA"
BASE COUNT         1663 a         1861 c         1610 g         1685 t
ORIGIN      
        1 aggtcgcgtt aaaacgcggc gtagggtcat actgaaagct tataagcacc gcggggtgag
       61 ttgccgcgcg cctctacagg gcgcgatgct cacgccccat gccttaactt caacgaaaca
      121 cctataccct gttgccaatt gcctgtttcc tattgcctca tgcctcgatt ataccatatc
      181 ttaggccaca tccggccagt ccggaggttt ttcatatcgg tgcaggaatt ttctgggata
      241 gctcgaagaa tatatatggg ggtgaaatat gatgagaatc ggcgacttgt taattcaagg
      301 cggtttcatt tccgaaattc agttggagag agctctggaa cttcagcgga cccagcccga
      361 gaagaaaatc ggcgagttac tcgtggaact tggttatgtg agttccgggg acatcataga
      421 tacgctcaag aaacagtgac cctgccccat tcatggttca agacgaaaaa aaaagcaacg
      481 gcccctactt caggtcgttg ctttttactc tctaaattat ttcgcggtta agcgatgatt
      541 tcactcatac ccccgcaaga gaaggagtct tgtctgtaac tttggattgc gatggcttcg
      601 actgcggagc gccgtatgct tcgcgatata cttcctcaat ctcggcaact aacggcatcc
      661 gcggattggt ccctgtggtt tgatcgttaa aggcaagatc ggccatttcc cggacttccc
      721 gttcgaaagc ctcccgatcc acccccgctt cggccaacgt caacgggata ttcaccttga
      781 ccatcagctc tttcacggcc ttgatcaaac tggccacacc ttgttccggg gtatcggccg
      841 gcaaaccaag gagccgggcg atctccgcat aacgctcccc ggcaatgggg tactcgtatt
      901 ggggaaatgc cataaacttg ctgggctttt tggcattgta cccgatgacg tagggtaaaa
      961 ggatggcatt ggcccggccg tgcggaatat gaaactttcc gcctaaagta tgggccattc
     1021 cgtgattgac tcccaggaag gcattggtga aggccattcc cgccatacag gaagcgttat
     1081 gcatcttttc cctggccacc cggtcctggc cgttttgata agcccgcggc agaaactcga
     1141 agaccatttt gatgaccttc tcagccagcg cgtcggtgta atccgaggcc atcaccgaga
     1201 cgtatgcctc aatcgcgtga gtcagcacat ccattcccgt atcggcggtt accttttgcg
     1261 gcacggtcat caccagatcg ggatcgataa tcgcgatatc cggagtcaac tcgtaatcgg
     1321 ccagcggata tttgatattc cgcttcttgt cggtgatcac cgcgaaggcg gtcacttcgg
     1381 aacccgtccc ggaggtggtg ggtatcgcca caaaggtggc tttcttgccc agatccggaa
     1441 atttgaaagc ccgcttgcga atatcggcga acttgagcct caaggtctca aactccaggt
     1501 ccggatgttc gtaaaagagc cacattccct tggcggcatc gattgcggaa ccgccgccca
     1561 gggcgatgat gagatccggt tggtaggcat tgagctcccg gcaaccattc atgaccgtgt
     1621 ccactgaagg atcgggctcc acttcggaga agatccggta gtcgaaccct atcttatcca
     1681 attggtaggt gaccttctcc gggaaaccca gtttcaccat gaagggatcg gtgacgatga
     1741 aggccctttt cccctttatt ttactgaggt actgcaatga accgtattca aaatagatct
     1801 tgggcggaat cttaaaccac ttcatccgat cgcggcggat cgccacccgc ttaatgttga
     1861 tcaagttacg gacggacacg ttatcggtgg ttgagttatg gcccatcgat ccgcaaccca
     1921 gggtgagcga gggggtattg gtattgtaca gatccccaat ggcgccgtga gaagagggcg
     1981 agttgaccag gagccgtccg gtgcgtagcc gccgggcgaa ttcatcaatc acttcctggc
     2041 tccccgaatg gatcaccgcc gaatgaccaa gtcccccgaa ctcggtgatc ttttcgcaaa
     2101 gtttaatccc ctcgtggtaa tcggcaacag tatagcaggc caaaatcggg ctgagtttct
     2161 cccgggagag cggatactcg ggtccgaccc cctcgagctc ggccacgagg atttttgtcc
     2221 cttccggcac ctcaaagccg gccatggcgg cgatcttcgc cgccggctgt cccaccacag
     2281 ccggactcat gctgtcgcgt tccggatcga tggcaaccgc cgccagtttc ccaatctcat
     2341 cgggtttcag gaaatagcat ccgtactccc gcatcaagtc tttcacccgg tcggccacgg
     2401 cccggtcgat caccaccgct tgttccgagg cgcagattac accattatca aaggttttac
     2461 tcaagatcag atccgagacc gcccgtcgca gattggcggt ttgctcaata tagcagggca
     2521 cattgcccgg accgaccccc agagccggtt tgccgcagga gtacgcggcc tgaaccatgt
     2581 tggtgccgcc ggtggctaaa atcatgctga tcccccgatg ctgcatcaaa agattggtgg
     2641 cttgaatcga aggttcggcg atccagccga tgcagttctc cggagcgccg gcggctaccg
     2701 ccgcatccag cattaccttg gctgctgcaa tgctggcccg aatcgccttg gggtgaaaac
     2761 tgaaaataat cgggttccgg gtcttgatgc tgattaagca tttaaacatc gtcgtcgaag
     2821 tgggattggt caccggcgtg actccggcga taattccaat cggctcggcg accttcatga
     2881 acccctcttc ctcgttttct tcaatgatcc cgacggtctt ctcattttta atattgtggt
     2941 agacctcttc ggtggcgaaa aggttcttgg cgatcttatc ctcgtagatt cccataccgg
     3001 tttcggtcgc ggccatccgg gctaattcca tatgcttatc gatcccggcg agagccatcg
     3061 ccttaacgat ggcgtcaatc tgttcctggt tatactccat aaaccggttc ttggcgatct
     3121 ccgcctgttt caccaactca tcgatgactg tggatatcct ttccgtggcg gctgtctttt
     3181 cggaaacgga tggctttccc tcaacctctt tgactggtga agcttgtacc ggtaacccct
     3241 cttttactac cgtatcatgc tgctcgtttc gggtatcctt ttttcccatt ttgatgacct
     3301 cctttgcgaa tatgagacct gaaattggtt ttcctgcggt tattcagggt ttttccccgc
     3361 ccacctccgc tcctgttcca gtttgaatga gagcacaagc aagctttcac taatatgtac
     3421 gtgttcaacc acgactgtag atggaacatt gagagctacc ggagcgaaat tccaaatccc
     3481 ctttaccccc ccgcgtacca acgtagtggc gatttcctgt gccaccccgg ccggggtggt
     3541 aatgatgccg atctccaccg gcgtctccgc caaatattcg gataatgcag ccaccggaag
     3601 caccgggatt ccggcgaccc tccctcccgg cgccggttga atatcgaaag cagccttgta
     3661 aataaaaccc cgttccgtaa agttggtgta gctgagcagc gcctgtccca gatggcccgc
     3721 accgaccagg acgacttgct tggttcggtc caggcccagg atcttattga cctgagagag
     3781 cagctcgccg gtatggtacc cgtaaccttg ctggccgaaa cttccgaaat aactaagatc
     3841 ctgccgcaat tgcgaagcgg ttattccaat cttggcggcc agttcctggg aggagatcct
     3901 ttcgtactcc tggtgtagca tttcgcttaa ataccgctgg tagactggaa gccgtttcat
     3961 caccgcctcc ggaattaccg ccgcttttcg ttccgatctc atttttctca tggaatctcc
     4021 tccgttatcg ttcgcttaaa aaatgacctc ttctcgtatc agtttgttaa aattttaact
     4081 aactgagcgc cggttgttca ttccatatac gcacgtcgta aataaattaa gctaaagaca
     4141 aaactaacat tatcaatccg tggtttacga agcaacaatc ttgaaccggg tggcaacttc
     4201 tacgttattc ttgatcatcg cccagacggg gcaaaacttt tcctcgctta accggattgc
     4261 cttttgcaga tcagcctctg ccgcatcccg ggaatcgaga ataaactcca aagttatctt
     4321 ctcaaaatag gtgggatgct cttcacgccg taccccttgg gcattcaccc ggaacccgga
     4381 gacatccttc ctcatcttcc gcagcaaaga aaccaccgtc gaaccgctgc aatcggcgag
     4441 acttaccaga aatagctcaa gtcccgtata accctggccg tcccccaaag gcggagcata
     4501 gtcgcaaata atcggttgat tggttcgagt ttttccttga aattgtactt tgtcgttgat
     4561 caactccgcc gaaaccgcta cgaattcgtt acccatttct acctcctcat tcactaatat
     4621 acgccgcgat taagcatgat atatggatta gaaggcaaat ttgttaaata tttaacaatc
     4681 atcttgcttg aattatatcc tgaatcggat caaaagtaaa taggatttca ccactttgtg
     4741 gtaaacaaat cagcaaccct ccccccgctg tcatgattcc attccgcgca tcagcggagg
     4801 agggcagggt gagggcttaa gaccgcggca acaaaataca ctcattgaca cttaccgcgg
     4861 tattctggcc gatttcaatc tcggccgctt cattctggtc cacctggaat tttaaaatcg
     4921 catcctcaat attcaggtac acgaagacat atccgcccat gaagatcttc tcaagtactt
     4981 tcccccgaaa attcacaccg gacgtacccg tattggagct gcaccattta atactctccg
     5041 gccgtatgca taacctggac tgaatcccgg ctttgatacc ttcggtgaaa accacatatg
     5101 ttttcggcaa tctcacaacc tgattggcaa attggagcac aaactcattg gcagtaactt
     5161 ctttgaccaa gccgtcgatg aaattgttgg aaccgataaa atcggcggaa aagatggagc
     5221 gcggccggtt atagacctcc agcggcgagc cctcttgttc aatcttgccc gagttcatca
     5281 ggaagatccg gtccgacatg gttaatgcct cgatttggtc atgggtaaca aagattgtag
     5341 tttttccggt ctctttctgg atagcccgca gttcaagccg gacactctcc cgtaatttcg
     5401 cgtcaagatt ggataagggt tcatcaagca gtagcacgtc gggatcaaag gccaacgccc
     5461 gggcaatggc cacccgttgt tgttgcccgc cgcttaactc cgctggtttt cgtttttgat
     5521 actcttccat atggaccagg cgcaagactt catcgacccg gcgggcaatg acatcaggct
     5581 ttgtcttttt catctccagg ccgaaagcta cgttttggaa aacggtcata tgcgggaata
     5641 acgcgtaatt ctgaaaaacc aggccgatat ttctttggtt gctcgggacc gtatcgatcc
     5701 ggcgactgtc aaaaaagatc tttccccggc tggggttgag caaaccggcg atcatcttca
     5761 gtgtagtgct cttgccgcaa ccgctcggtc ctaaaagggt aatcagttcg ccatctttga
     5821 tttcgaaaga aatatcatta attacgctag tttctccaaa actcttcgaa acgttttcaa
     5881 atcgtacagt tgccatcgtt gtcctcctct agaaaacgac ttcaatttga gtccaataat
     5941 caattattct gagtggtttc acaagacgtg cccaagggca aacttagccg aacccgattc
     6001 ggaaactatg catccttctt ggcgaagact actccactca aataagcagg tcctaaaaac
     6061 acgctgatta aaacataaaa taggaccgct acaaaaatca agatcgccgc catggcaatc
     6121 gaatgcggca gagcccctct ttccacgata tcgctgaata accgaattga aattgggctc
     6181 gacatcggcc cggctataaa gaagatgatc aaaaaactca tcaggtttga catgaataca
     6241 aataaggcgc tgacaataat cccaggtccc atcaacggta agacgatccg ggtaaaaacc
     6301 gtcattttcc cggcgcctaa agtcatggcc gcctcttcat acgtggcgtt aaccccttcc
     6361 gctacagcca aagtataacg gtaagccaat ggcaagccat tggcgcccac cagggcaatg
     6421 agtagtccta aaaaattatt ggtcagatgg atcggcagaa agaaccttaa taaagcaata
     6481 gcggtcacga ttttgggaat taaaatcggt aaaagaacga tattggacaa tagattttta
     6541 ccgggcagtc tccccctgac cattgcccag gaagccggag cagcgaccat aaccgaaaca
     6601 atcgcagcca gtaccgccag ccagaggctg aaaataaagg aatcccaata ggaacggggg
     6661 attgaataat aagccttcaa tgaaagcgac ttcggaatca aactaatatc cagcgttcct
     6721 tggaaagaag catagacgat taaaaagagc gggaaggcga accaaatcac catcatcccg
     6781 aggtaagaat agaacccggc tttcgataac gtttttctc
//
LOCUS       DASVMB010000561         9710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_67807,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000561 DASVMB010000000
VERSION     DASVMB010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67807"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VF531_15700"
     CDS             complement(<1..936)
                     /locus_tag="VF531_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3658 domain-containing protein"
                     /protein_id="HEX7715462.1"
                     /translation="MIEIVFNDSACGSLKTAQHYGEGKYQGGCIGVIVSHANGNKPTK
                     EEVEAAQQEAREKACLAWESATPLGGNTADIYGFNLVLSVGDISENQPGIKRRQTLEN
                     LYSVYPNDEGHQAAQEILKRVNEDLKTVQERAAAGESLRIWYSNQPDEMCGLYWFMGQ
                     LNQWKVHGRQVSIVKLPEWEADEKGNIVQKSGWGEVAPGEWHRYLALQKPVLPVFEQS
                     CASHWQELQRENAPLRATLNGQLISAPETLYDGFILREIAAEGEEFQEAMVIGRVIGK
                     YQLGIGDSWIAFRIEEMIRAGKLESVTAVAKDMPIY"
     gene            complement(1379..2212)
                     /locus_tag="VF531_15705"
     CDS             complement(1379..2212)
                     /locus_tag="VF531_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715463.1"
                     /translation="MFKQFTMNQKELPRLWLSIGLVMLFLLALNLVGASAQENISRDL
                     GGAVRIYASANSAYEQSRYNQALEGYNQLLKAGFNGGGLYYNLGNTYFKLYDKGHAVL
                     YYEKAMRLIPRDTALQANLTLALNGVEEGRPGWVRESYRALVTLAPLNQLALAGSVCF
                     FVLMIGLCFRSLFQANHTLGSPGKFRKLWQGIVVLMGMVTVLCWAMTFLTYIDQHQDQ
                     GVIVKPAVAYYETLPDAATAFQLAPGSRLRILKQTGEWSYIERRDGKRGWVRMSCYEE
                     I"
     gene            complement(2205..3752)
                     /locus_tag="VF531_15710"
     CDS             complement(2205..3752)
                     /locus_tag="VF531_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX7715464.1"
                     /translation="MGRGCSSKVSGLKILGLAFLICIGLTCGFPTQTLRAEDAAGSGV
                     ISQTDTGEYQPYFVRLELPKTRLYQGEQVPLSINLYITGLNVVEVNSPLLNQKELAFG
                     TLSRASQSTQTIGGHKYRVVRFSTTITPLKTGKLVLNPITVICKIGQKYAVTMADGRQ
                     YERVVQKNLEVASAQTRLNILPLPESNQPVDFGGGIGQFRINAYRNSTNPIRQGEPFK
                     VQLTVTGRGNLSSIGMPYLKTSPLIQSFAAERKPSTRSDQAVYETTVVVQDAKIKNIG
                     PFLFVYFNPNNGKYETAQAALPVTVTIVPGTSSNQTEQSPRSKDDAGAKLAPVKENAT
                     ELNSSPFILLEEFWFWLLQLIPILLWIVCMSYRNYQRMLKSDLPQSRAIKAAAKSGKR
                     LNEAKILLDEGKYEGLLEELHFILREFLSERFNLAASGMTGAVASVLAEKEDDLPPQA
                     LEDIKAFFAEYDRHRFAGTEFQRREALRLWEIVNSVIAAINRIPTERSNKEITTASGT
                     KGGSNHV"
     gene            complement(3813..4475)
                     /locus_tag="VF531_15715"
     CDS             complement(3813..4475)
                     /locus_tag="VF531_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7715465.1"
                     /translation="MKRQVTSPLMLKIGAVLIYLVLLVALVWGPTIRNAQANQNFRKG
                     NYPQARDEYRRFTGGEDVSPVILHNLGISWYQLGRFAEARSCLDQACTKYTAQVRSRK
                     GSTQGNPITARFHYHLGNALFQIAAQSSIMRADTEITAENRKKMEQAMADYAAALGEY
                     KKALLANPNDFEAKYNYEITLARLGQSPPPQQQERPPVQSEDQYLEENHEDVPPPNGK
                     DW"
     gene            complement(4472..5503)
                     /locus_tag="VF531_15720"
     CDS             complement(4472..5503)
                     /locus_tag="VF531_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7715466.1"
                     /translation="MSLSYPYFGFLFLIPLLGVFFLTFIRQKNARDLQRFASPEIFGK
                     ILDDSAARTRLYRGVLRIICLFVLVLALIGPELGYNWREQKGQGLEIVFALDTSKSML
                     ATDIQPSRLERAKLAIKNLLPQIAGNKVGLVAFAGTAFLQSPLTVDFNGFVASLDGLG
                     VQSIPRGGTAIGSALQVCLKAFQSGEAAPKIVILMTDGENHEGDPVSLAQAAVKQGIN
                     VYTIGIGNPSGVTIPVTDANGKTTFIKDSDGKVVKTKLNEKILRAIAQAGKGSYIRGN
                     DVSMGLTELYRTGLSKYQRKKLSASEKWQKEPINRYQWPLGIAIIFLAIEICLGRVST
                     FRKVKGVRV"
     gene            complement(5500..6507)
                     /locus_tag="VF531_15725"
     CDS             complement(5500..6507)
                     /locus_tag="VF531_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7715467.1"
                     /translation="MIFANPLFLFGLLVVPPLAFLIIYHRVKPGSGTIHYSDIRILAA
                     IKPGLKVRLANGLPWLSLLALVLMIVALARPQMGLEQSRIRHAGVDVILAIDVSSSMG
                     ARDFTLSGRQANRLDAVKLVARNFIKNRPNDRIGIVVFSGRPYILAPLSWDHDWAISR
                     LSEIKPGTVEESGTAIGAALATSINRLQDSTAKSRVIILLTDGMNNSGLVAPETAAAS
                     ARKAGMSLYTIGAGSRGVAPVPVIGTNGHVTYQNQAVNIDETLLTQLAESTGGRYFRA
                     TDTRSLSVIFERINRMAKTSMEAPKFNDYLELYPYFLVAALLLLCGESLLANTVFRRL
                     P"
     gene            complement(6556..7629)
                     /locus_tag="VF531_15730"
     CDS             complement(6556..7629)
                     /locus_tag="VF531_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715468.1"
                     /translation="MVRKTQSSTKKRKFWIAGSLLLLGAAIGVWFALTNGARLAPLAL
                     PQGEMLWGRIGVEAIGPFTIGDQIPVTFEVEAKESVDCRLPDLSKSFSQNHLEVIRKY
                     PLRSQSLPGGVRKTVRYVVVSWQAGRFTLPAVKLRYTYQNAKRIYTITGQTLEVVSVL
                     PPRKSAEQLKALAIKGVKQPLSLPPRYLVLGCYLLATLLVSALFWSLRTLKKHQETAP
                     VEPELPPEPADVIALRRLEIIKSSNYIGEKKFLVFYTELSECIREYMENRYRINALEM
                     TTEEFLQHLTGDSRLPETEQQVLKEFLSSADLVKFAKYIPAETEANQALAAIEQLILA
                     TREEIVSEPASDDVVAIEAPPTN"
     gene            complement(7767..8645)
                     /locus_tag="VF531_15735"
     CDS             complement(7767..8645)
                     /locus_tag="VF531_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7715469.1"
                     /translation="MLPKEILKKVKKIQLKIARMSTDVLSGSYISAFKGTGVEFDEVR
                     EYIPGDDIRSIDWNVTARSGTPFIKRYVEERELTMILMVDLSASERFGSGTALKSELA
                     AEIAALLAFLAIKNNDKVGLLIFSDTCEKYIAPQKGRGHVLRVIREILGYEPHSTGTN
                     IAQALNFINKVTNHRSIIFLLSDFLDTEFEGAIKSLAKKHDLVTVRLTDPRELELPAL
                     GLMEFEDNETGKRVLLDTSAPSVRQQVQNAVLERNENVSRIFKNNKIDMIEIATDQSY
                     LEPLQKFFLRRERRRH"
     gene            complement(8737..>9710)
                     /locus_tag="VF531_15740"
     CDS             complement(8737..>9710)
                     /locus_tag="VF531_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7715470.1"
                     /translation="AINEEVKAKSGFVAKLKKQIGMVIVGQEKLIDSLLIGILGGGHV
                     LLEGVPGLAKTLSVKSLSQSIHADFQRIQFTPDLLPADLLGTLIYNQQSGDFVTKKGP
                     IFAQIVLADEINRAPAKVQSALLEAMEEKQVTIGDTTYPLPKPFMVLATQNPVEQQGT
                     YALPEAQLDRFMLKIKLNYPNHQEEREIMERMAKTNRRTELEPVITPEEILEIRQVVD
                     AIYMDPKIKDYIVDLVVATRQPDQYHLDLAAYIDYGVSPRATINLAMAAKAYAFIQGR
                     GYVVPNDVKAIGPDVLRHRLIVSYEAEANDLGVDDLIARLFDGVEVP"
BASE COUNT         2591 a         2457 c         2018 g         2644 t
ORIGIN      
        1 atagatcggc atatccttgg caaccgcagt cacagattca agttttcctg cacgaatcat
       61 ttcttcgatg cggaacgcga tccaggaatc gccaattcca agctggtatt taccaatcac
      121 ccgcccgata accattgctt cctgaaattc ctcaccttct gctgcgattt cacgaaggat
      181 aaagccatca tagagggttt ccggtgcgct gatcaactgc ccattcagcg tggcccgcaa
      241 aggtgcgttc tccctttgaa gctcctgcca atgagatgca cagctttgtt caaataccgg
      301 tagcacaggc ttttgcaagg caagataccg atgccattct ccgggagcaa cttcacccca
      361 gccgcttttc tgcacgatat ttcccttttc gtcagcttcc cattccggga gcttgacaat
      421 agaaacctgt ctgccatgca ctttccactg attcaactgc cccatgaacc aatagagtcc
      481 acacatttca tcaggctgat tgctgtacca gattcgaagg gattctcccg ctgctgcgcg
      541 ttcctgaacc gttttcagat cttcatttac ccttttaagt atttcctgag ccgcctgatg
      601 cccctcgtca ttcggataaa cgctgtataa attttccaac gtctgcctcc gcttgatacc
      661 gggctgattc tccgaaatat ctccaacgct cagcaccaaa ttgaatccat agatgtctgc
      721 tgtatttcca cccaaggggg tagcgctctc ccacgccaag catgcctttt cccttgcttc
      781 ttgttgagca gcttcaactt cctctttggt tggcttgttt ccatttgcgt gactaacgat
      841 aacgccaata cacccacctt gatatttccc ctcgccatag tgctgtgccg ttttaagact
      901 tccacaagca ctatcgttga atacgatctc aatcataata attattctcc aagtctcgga
      961 tttacccatc cgcaagatta tataaataat ccgtatggca aatttctgtt tatctgcata
     1021 ttaaaataat cgaaatctga acatcgcatt taactatatc ataccagcat aaatctttcc
     1081 accgaattat tatcaataat ggcaatagct tcctggaaca ctatgtgggt atcctgtgtc
     1141 actaacggga ggtagatttt cctatttaca attacttgta aagaattttg taacccgcca
     1201 tctcttcctt cttaattttc acaaatactt taaattaaaa aataaaagcc ggcatatcgc
     1261 cggcttgttc atagaatcta taaaagcatt atcttcaact ttcgggatat aatacctttg
     1321 accaccagag gccagggtta cccttccctc tgaatctgct tcctgaaata gcttaaccct
     1381 aaatctcctc ataacaactc atccgcaccc agccgcgttt cccatctctc cgttcgatat
     1441 aactccattc ccctgtttgt ttcaaaatcc gtaatcttga acccggagca agttgaaatg
     1501 cggtagcagc atccggcaat gtttcataat atgccactgc aggtttgacg attactcctt
     1561 ggtcttgatg ttgatcgata taggttaaaa aggtcattgc ccaacataaa acagttacca
     1621 ttcccattaa aaccacgatg ccttgccata atttccggaa tttccccgga gaacccaaag
     1681 tatgattggc ttgaaaaagg cttcggaagc ataggccaat catcaataca aaaaaacaaa
     1741 cggaacctgc taaagccagt tgattgaggg gggctaaggt taccaatgcg cggtaagatt
     1801 cacgaaccca cccgggtctt ccttcctcca cgccgttcaa ggcaagagtt agatttgctt
     1861 gaagagcggt atctctagga attagccgca tagctttttc ataatataaa actgcatgac
     1921 ctttatcata tagcttaaaa taggtattcc caagattata ataaagaccg ccgccgttaa
     1981 aaccggcttt taataattga ttgtagccct ccagggcctg attataacgg ctttgttcat
     2041 aagcactatt cgctgaggca taaatccgta ctgctcctcc caaatctctc gaaatatttt
     2101 cttgggctga agcgccaact agatttaaag ccaacaaaaa taacataacc aatccaattg
     2161 acaaccataa acgcgggagt tccttctgat tcattgtaaa ttgcttaaac atggtttgaa
     2221 ccccctttcg tcccggaagc agtagtaatt tccttgttgc tccgttcggt aggaattcga
     2281 ttgatcgctg caatcaccga gttcactatt tcccataagc ggagcgcctc ccggcgttga
     2341 aattccgttc ctgcaaaacg atgcctgtca tactcggcaa aaaaagcttt gatatcttct
     2401 agagcttgag gaggtagatc atcttctttt tctgcgagga ccgaagcgac cgctccggtc
     2461 attccggagg ccgccagatt gaaccgttcg cttaaaaatt ctctaagaat gaaatgaagt
     2521 tcctctaata gcccttcata ctttccctca tccaaaagta tcttggcttc gtttaaacgt
     2581 tttccggatt tagctgcggc cttgatagcc cgagattgcg gcagatcgga tttaagcatc
     2641 cgctgataat tacgataact catacacact atccataaaa gtatgggaat taactgcagt
     2701 aaccaaaacc aaaattcttc cagcaatata aaaggagaag aatttaattc cgttgcattc
     2761 tctttaaccg gtgcgagctt cgcaccggca tcatcctttg atctcgggct ttgctccgtt
     2821 tgattggaac tcgtccctgg aacaatcgta actgtaactg gaagtgccgc ttgtgccgtt
     2881 tcatacttac cgttattcgg attaaagtat acaaacagga acggaccgat atttttaatc
     2941 ttggcgtctt gaaccacaac ggttgtttca taaactgctt gatctgagcg ggtggaaggt
     3001 ttccgctctg cagcaaagct ttgaatcaaa ggggaagtct taagatacgg catgccgata
     3061 ctgctcaaat tccccctgcc ggtaacagtt agttggactt taaaaggttc tccctggcgg
     3121 atcggattgg tggagtttcg ataggcattg atccgaaact gtcctatccc acccccaaaa
     3181 tcaacgggtt gattactttc aggcagaggt aggatattca gtcgagtctg tgcactcgca
     3241 acctccagat tcttttggac aacccgctcg tattgtcttc catccgccat agtcactgca
     3301 tatttttgac ctattttaca gatcacagtg atcggattca aaacaagctt cccggtcttg
     3361 agcggagtta ttgtggtgct aaatcgaacc acgcgatact tatgtccacc aattgtttgg
     3421 gtagactgag atgcccggct aagagtcccg aaagccaatt ctttctgatt gagcagagga
     3481 gaatttacct caaccacatt taatccagtg atataaaggt tgatggaaag cggaacctgt
     3541 tccccttgat ataaacgggt cttaggcaat tccaatctta caaaatacgg ttggtattcc
     3601 ccagtatcgg tttggcttat gactccggaa cccgcagcat cttcggctct caaagtctgc
     3661 gtggggaagc cgcaggttag cccgatgcag atcaaaaaag cgagtcccaa aattttcaaa
     3721 ccggatactt tgcttgagca tccacgcccc atataatcct ccttgttgtt ctgcactttc
     3781 atgccataac ctttcttaaa ataacattat ctttaccagt ccttcccatt tggcgggggg
     3841 acatcctcat ggttttcctc tagatattga tcttccgatt gaaccggggg ccgctcttgt
     3901 tgctggggag ggggtgactg gcccaatcgg gcgagagtaa tctcataatt atactttgcc
     3961 tcaaaatcat tgggattggc gagtagagct tttttatatt cacctaaagc tgctgcataa
     4021 tcagccattg cctgttccat tttttttcta ttctcagcgg taatttccgt atctgcacgc
     4081 atgatactgg attgagcagc aatttgaaat aatgcatttc caagatgata atggaaacga
     4141 gctgtgatag gatttccttg agtgcttcct tttcgggaac ggacttgagc tgtgtacttg
     4201 gtgcaagcct gatccaaaca agaacgggct tcagcaaagc ggcccaattg gtaccagctt
     4261 atacccagat tatgaagaat aacaggagaa acatcttctc cccctgtaaa ccttcgatac
     4321 tcatcccggg cctgcggata gtttcccttc cgaaagtttt ggttggcttg agcattccgg
     4381 atggtaggcc cccaaactaa agcaaccaac aggaccagat aaataaggac ggctccgatt
     4441 ttaagcatga gaggtgatgt aacttgccgc ttcaaaccct aacccccttt accttacgga
     4501 atgttgaaac tcgacccaga cagatttcga ttgcaagaaa aatgatggcg atgcctaacg
     4561 gccactggta gcgattgatt ggttcctttt gccacttctc cgaagctgaa agctttttac
     4621 gttggtattt ggaaagtccg gtacgataga gctccgtaag tcccatcgat acgtcgttac
     4681 ctcgtatata actccctttt ccggcttgag ctattgctcg caaaattttc tcattaagct
     4741 tggttttgac aacctttcca tcactatctt ttataaatgt agtttttccg tttgcatccg
     4801 tgaccggaat tgtcacaccg gaaggattcc caatcccaat agtataaacg ttgattcctt
     4861 gctttactgc ggcttgggct aaggaaaccg gatcaccttc atgattctcc ccatctgtca
     4921 ttaatattac aatttttggt gcggcttccc cagattgaaa agctttaaga cagacttgaa
     4981 gcgcggaacc gatagcagtg ccgccccggg gaatcgattg cacccctaaa ccatccaatg
     5041 aagcgacaaa tccgttaaaa tccacggtca aaggagattg caaaaaagcg gtcccggcaa
     5101 aagccaccag gcccacctta ttcccagcaa tttggggtaa taaattctta atcgccaatt
     5161 tggcgcgttc taaccggctg ggttggatat ctgtcgccaa catgcttttg gatgtatcta
     5221 aggcaaatac gatctcaagt ccctgccctt tttgttcccg ccaattgtat cctaattccg
     5281 gtccgatcaa tgccaagact aaaacaaaaa gacaaatgat ccgcaatact ccgcgataca
     5341 acctggtccg ggctgcggaa tcatccaaaa tctttccgaa gatttcaggg gaggcaaatc
     5401 gttgtagatc ccgtgcattc ttctgccgaa taaaggttag aaagaagact cccagcagag
     5461 gaattagaaa taaaaaccca aaatacggat aactcaagct catggcagtc tcctgaaaac
     5521 tgtattggcg agtagcgact caccgcaaag tagtaataat gccgcaacta aaaaatatgg
     5581 atagagctca agataatcat tgaacttcgg cgcttccatc gaggtcttcg ccatccggtt
     5641 gattcgctcg aaaatcactg aaagactccg ggtatcggtg gctctaaagt aacggccgcc
     5701 ggtactctcg gcaagctgtg tgagtaatgt ttcatcaata ttgacggctt gattttggta
     5761 ggttacatgc ccatttgtac caattaccgg aaccggagca accccacgcg aaccggctcc
     5821 aatagtatat aatgacatcc cggctttccg ggcggacgca gctgctgttt ccggtgcgac
     5881 tagaccggag ttgttcatgc catcggtcag taaaataatg acccggcttt tagcggttga
     5941 atcctgcaaa cggttgatgg atgtagcaag agcagcgcca atggctgtac cgctctcctc
     6001 aaccgttccc ggcttaatct ctgaaagcct cgaaatagcc caatcgtggt cccagcttaa
     6061 aggagcaaga atataaggcc gacctgagaa gactacgatc ccaatccgat cattgggacg
     6121 gttttttatg aaatttcgtg caacaagttt tacagcatcc aaacggttgg cttgtctgcc
     6181 tgaaagtgtg aaatcccgtg cccccatact acttgagacg tctatagcca ggatcacatc
     6241 cacccctgca tgacgaatcc gactttgctc caaacccatt tggggtctgg ctaaggcaac
     6301 aatcatcaag acaagagcga gtaaggaaag ccaaggcaga ccattggcta aacgtacctt
     6361 cagaccaggt ttgatcgcag ctaaaatccg gatatccgag taatgaatcg tacccgagcc
     6421 aggcttcacc ctgtgataaa tgataaggaa agcgagggga ggcacgacaa gaagaccgaa
     6481 taaaaataaa ggattggcaa agatcatgat tggctcctga aaagtattta gccccttaag
     6541 ggtaacctcg cggggttaat tggttggagg cgcttcgatc gctacgacat cgtcacttgc
     6601 gggctcggaa acgatctctt cccgtgtggc taaaatcaat tgttcgatcg ccgctaaagc
     6661 ctgattggct tcagtctccg ccggaatata ttttgcaaat ttgaccagat ccgccgaact
     6721 taaaaattcc tttagaacct gttgctccgt ctcgggaagc cgactatcgc cggtcagatg
     6781 ctgcaggaat tcttcagttg tcatctcaag agcgttaatc cggtaccggt tttccatata
     6841 ctcgcgaatg cactcactca gttcggtata aaaaacaaga aactttttct caccgatgta
     6901 gtttgagctt ttaataattt ccaagcgtcg taaggcaatg acatcagctg gttctggagg
     6961 aagttccggc tccacgggtg ctgtctcctg atgtttctta agagtccgta agctccagaa
     7021 caaggcagaa acaagaagag tcgcaagtaa gtaacatcct aataccaaat atcgaggagg
     7081 tagcgacaaa ggttgcttga ctcctttaat tgccagagct tttaactgtt ctgcggactt
     7141 acgcggcggt aagactgaga caacttccaa agtctgcccg gttattgtat aaattctttt
     7201 tgcattttga taagtataac gtaattttac tgcgggtaaa gtaaatcgac ccgcttgcca
     7261 ggaaactact acgtatcgaa ccgtcttacg aactcctcct ggtaaactct ggctccgtag
     7321 cggatacttc cgaatgacct caagatgatt ctgggagaaa ctctttgaca ggtccggcaa
     7381 ccggcaatca accgattctt tggcctcaac ctcaaatgtc actgggattt gatctccaat
     7441 ggtgaaggga ccaatggcct caaccccgat ccggccccag agcatctcac cctgaggaag
     7501 agccaacggc gccaagcggg caccgtttgt cagagcgaac caaaccccga tagccgctcc
     7561 caaaaggagt aaactcccgg cgatccagaa ctttcttttc tttgtggaac tttgcgtttt
     7621 cctaaccatt cgtaatggtt acccccggat tcgtaacata caagcttatc gaaaaccaca
     7681 ctcaaaacgg gtctggttta acagctggta tcaacctgga tccactaaac aacttcaaga
     7741 ttcatattat cttgccgaaa atgggttcaa tggcgacgcc gttcccgtcg caggaaaaat
     7801 ttctgtaaag gctctagata tgattgatcc gttgcgatct caatcatatc aatcttattg
     7861 ttcttgaaga tccgtgaaac gttttcattc cgttcaagga ctgcattttg aacctgctga
     7921 cggaccgatg gagcactggt atccaacagc acccgttttc cagtctcatt atcttcgaac
     7981 tccatcaacc ccagtgccgg aagttccagc tccctgggat cagttagccg caccgtcacc
     8041 agatcatgct ttttagctaa acttttaatg gctccttcaa actcagtatc caaaaaatcc
     8101 gataataaaa agatgatgct ccgatgattg gttaccttat taatgaagtt taaggcctga
     8161 gcaatatttg ttccggtgct gtggggctcg taacccagga tctcccggat tacccgtaaa
     8221 acatggcctc ttcctttttg aggtgcaata tatttctcgc aggtatcact gaagatcaag
     8281 agcccaacct tatcattatt cttaatagcc agaaaggcca gtaacgccgc gatctccgca
     8341 gccaactcac ttttaagtgc agtcccggaa ccaaaacgtt ccgaagcgct taagtcgacc
     8401 atcaagatca tagtcagctc gcgttcctca acataccgtt tgataaaggg agtcccgctc
     8461 cgggcggtga cattccagtc aatcgaccgg atatcatccc ccggaatata ctcccttacc
     8521 tcatcaaact caaccccggt tcccttaaaa gcgctgatat aagagccaga cagcacgtct
     8581 gtgctcatcc gggctatttt cagctgaatc ttcttgacct tcttaagaat ctccttagga
     8641 agcatacgtt acctcgccgt tcataatgta tcggcgcccc gcgggggcgc ttcctaccac
     8701 catttgtacc atgcgcccca tggggccgct tctacattaa ggaacctcta ctccatcaaa
     8761 gagccgggca attaaatcat ccactcccag atcgttcgct tccgcttcat aggagacgat
     8821 caaccgatgg cgtagaacat cgggacctat ggccttcaca tcgttgggaa ccacataacc
     8881 acggccctgg ataaaggcat aagccttggc tgccatagcc aaattaatag ttgcccgggg
     8941 tgaaacacca taatcaatat aggctgccaa atccagatga tactgatccg gctggcgcgt
     9001 tgccacaacc aggtcaacaa tataatcctt aatcttggga tccatataga tcgcatctac
     9061 cacttgacgg atttctaaga tttcctcagg agtgataacc ggctcaagct ctgtgcggcg
     9121 gttagtctta gccatccgtt ccataatttc tcgttcttct tgatgattgg gataattcaa
     9181 ttttatcttg agcataaagc ggtctagctg tgcttcgggt agtgcgtaag tgccttgttg
     9241 ctcaacaggg ttttgggttg ctaaaaccat aaagggtttc ggcaacggat atgtggtatc
     9301 accgatggtt acttgttttt cttccatagc ctcgagcagg gcgctttgaa ctttggccgg
     9361 agcacggtta atctcatctg ctaaaacaat ctgggcaaat atcggtcctt ttttggtaac
     9421 aaaatcaccg gattgctgat tgtagatcaa tgttcccagt aaatcggcag gaagaagatc
     9481 cggtgtaaac tgaatccgct ggaaatccgc atgaatgctc tgggacaaac tcttgacgga
     9541 taatgtcttc gcaagacccg ggaccccttc aagcagtacg tggcctccgc caagtatccc
     9601 gatgagcagg ctatcaatga gtttttcctg tcctacgatg accatgccaa tctgtttttt
     9661 cagtttcgcg acaaaaccgc tcttggcttt aacctcttca ttgattgctg
//
LOCUS       DASVMB010000562         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_658582,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000562 DASVMB010000000
VERSION     DASVMB010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658582"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VF531_15745"
     CDS             <1..879
                     /locus_tag="VF531_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715471.1"
                     /translation="PVLHLNHDNQDKLEISDDARNALKDAQKVDPAQVDQSQTHLQPN
                     EKLKVDVFSTTLKAIGDFQMRQAIFIPARPLVVQTECREVYMESEKMSFKAQGKIKTA
                     DGKEIDFSSELNMSREFVTDHITNINQRLLDPLVVNFDGPAAALTDTKFSFDLDNDGT
                     SDQISSLNLGSGFLALDQNQDGVINNGSELFGTKSGNGFADLARYDLDQNGWIDENDA
                     IFDKLRIWTKDQSGRDVLFGLGQKGIGAIYLGSVGTQFSLKNEANQTQGEIRSSGVFL
                     REDGTTGTMQQIDLAI"
     gene            1144..1590
                     /locus_tag="VF531_15750"
     CDS             1144..1590
                     /locus_tag="VF531_15750"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7715472.1"
                     /translation="MSDVFDYNFISDVIMDLLKKYNKMESSRYIYRDYPLTMSEIYYI
                     LAIQNSQKKSMTDLAKNVDVNLSTATSMIDKLTRNGLVERIRDLEDRRQVFVQLTSKG
                     EEIYTSIIESRKIVMEKLFSLFTPEEKIWVYHILLKLHQKINDVEL"
     gene            1739..2947
                     /locus_tag="VF531_15755"
     CDS             1739..2947
                     /locus_tag="VF531_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7715473.1"
                     /translation="MKIPSNASQNQGTSPQEIAPGVWMLEGFLSNDFMGSPPSCNVYF
                     LRDGETLLILDTGHGNSSREDILEFIKKMEKEGIQRLILMVTQGHFDHSGNNDLLMET
                     GLDWEFRLPEPEFPVIDVFNDFLNDLGNLEKYENIYRTMFPLKGKTAVIRIVGMFAPG
                     LAKIILRKVFRRQFSKCHPLAEKACPLKLKERMTMEFGSVTLTGWKVGRFFIIHDGAH
                     SPGHICLYDPDNRLLLCGDVTIEVNPAFLYSSINKLIETTERFNTMAEEGFIKIVGDS
                     HRTRKYFTILCKQLGFQMLNEIQLIEYAVGKDQCIRFFSTFHSYYQQLKKEVLAAHEK
                     VGRATVGKIVKELEKSSNPAVKLKTALVFPQVPSRMDVLVASVLKEAGAVPVRERGKI
                     VIDPVGIKSI"
BASE COUNT          918 a          620 c          725 g          843 t
ORIGIN      
        1 cccgttctcc atctcaatca tgacaaccag gataaactgg agatctcgga tgacgcccgg
       61 aatgcgctga aagacgccca aaaagttgat ccggctcagg ttgaccagag ccaaacccat
      121 ttacagccca atgaaaaatt gaaggtagat gtttttagta ccacgctaaa agcgataggc
      181 gatttccaaa tgcgtcaggc catcttcatt ccggcccgcc ctctggtcgt tcagaccgaa
      241 tgtcgtgaag tctacatgga atctgaaaaa atgtccttta aagcgcaggg gaagatcaaa
      301 accgccgacg gcaaagagat cgatttcagc agcgaactta atatgagccg ggagtttgtc
      361 accgatcata ttacgaacat caatcagagg ttgctggatc cgctggtggt taattttgac
      421 ggtcctgcgg ccgcgctgac agataccaaa ttcagctttg accttgacaa tgacgggaca
      481 tcggatcaga tttcttcact caatctcggg agcgggttcc tggcactcga tcaaaatcaa
      541 gatggtgtca tcaacaacgg cagcgagctt ttcggcacca agagcggcaa cggctttgcc
      601 gatttggctc ggtacgatct agatcaaaac ggatggattg atgaaaacga tgccatcttc
      661 gataaattac ggatctggac caaagatcaa agcggccggg atgttttatt cggccttggc
      721 cagaaaggaa tcggcgctat ttatctcggg agcgtcggca cccaatttag cctgaaaaac
      781 gaggccaacc aaacccaggg agaaatcagg agcagcggcg tctttctccg ggaggacggc
      841 accaccggaa ctatgcagca gattgatctg gccatttgac cggagttata aaataagggt
      901 ttatccaacc aacatttttt gatttgcttt ggatgacagc cgatcggagg gttaatctct
      961 gattggctgc ctttttatag tgaaactaac cgtgcctttc gttcggagta tttggtttat
     1021 tctgaagatg ccgggtctga cgccttagtt atcggggatt gagctacttt acatcggcca
     1081 ggattttata tataattaga ttatcaaaat attaagttct cgaatgaaat ggggaagtca
     1141 catatgagcg atgtgtttga ttacaatttt atcagtgacg ttattatgga cttattaaaa
     1201 aaatataata aaatggagag ctcccgctat atttatcggg attatccatt gaccatgagc
     1261 gaaatctatt atattctcgc catccaaaac agtcaaaaga aatctatgac agatctcgcg
     1321 aaaaatgtag atgtaaattt gagtacggca acttccatga ttgacaagct tacccgcaac
     1381 ggattggtgg agaggatacg ggatctggag gatagaaggc aagtctttgt ccaattaact
     1441 tcaaagggag aagaaatcta tacttcaatt attgagagcc gaaaaatcgt catggaaaag
     1501 ttattctcct tatttacacc ggaggaaaag atttgggtat atcatatttt gctaaagtta
     1561 catcagaaga tcaatgatgt ggagctatga tttttttata aaaatagttc gaatatcgaa
     1621 tcattagatg atccaatgaa acattgaaag tgaagcgacg tcctgcaaca agtgttggag
     1681 ttaccaattc atctgaaatc caaatctgat tcaaagatca aaatggaggt gtgcgactgt
     1741 gaagattcca agtaacgctt cccaaaatca aggaacgtct ccgcaagaga ttgcccccgg
     1801 cgtatggatg ctggaaggat ttttgagcaa tgattttatg ggaagtccgc catcttgcaa
     1861 tgtttatttt ctaagggatg gcgaaacttt attaatattg gataccgggc acggcaattc
     1921 atccagggag gatattctgg agttcattaa aaaaatggaa aaagaaggaa ttcaacgcct
     1981 gatactcatg gtcacccagg gccatttcga tcattccggc aataatgatc ttcttatgga
     2041 aacagggttg gattgggaat tccggctgcc cgaacccgag tttccggtaa ttgatgtatt
     2101 caatgatttt ctgaatgatc ttggcaattt ggagaaatat gaaaatattt accggaccat
     2161 gtttccgctg aaagggaaaa cggctgtcat ccggatcgtg ggaatgtttg ctcccggcct
     2221 ggctaagatc attttaagaa aagtttttcg caggcaattt tcgaaatgtc atcccctggc
     2281 ggaaaaagct tgtccgttaa aacttaagga aaggatgacg atggaattcg gcagtgttac
     2341 cttaaccgga tggaaagtag ggcgattttt tatcatccac gatggcgctc attcccccgg
     2401 ccacatttgc ctttatgatc ccgacaatcg gttgttgctt tgtggggacg taacaattga
     2461 agtcaacccg gcctttttat acagttcaat caataaactg atagagacga cagaacgatt
     2521 caacactatg gctgaagaag gcttcatcaa aattgtcggc gattcgcacc gtactcggaa
     2581 gtactttacc attttgtgca agcaattggg atttcaaatg ttgaatgaga ttcaattgat
     2641 tgagtatgca gtgggaaagg accaatgtat caggttcttt agcacttttc actcatatta
     2701 ccagcaactt aagaaagagg tattggcagc ccatgaaaag gtgggcaggg ctacggtagg
     2761 taaaatcgta aaagaattgg aaaaatcatc gaatccagcg gtaaagttaa aaacagcgct
     2821 ggtttttccc caggttcctt cacgtatgga tgtgctagtg gcttccgtac ttaaggaggc
     2881 cggtgccgtt ccggtgcgag aacggggcaa gattgttatc gatccggtgg ggattaaatc
     2941 tatttaaagg ggacattatc agaacaacta tgagaccacg atacatactt atctcatata
     3001 tcactttgat tccttccttt caaactttct cgattcgaac agccaattta aatttgttgt
     3061 tgcctccatt tttctttggg aatgttaaaa tcaccggaga tagtcg
//
LOCUS       DASVMB010000563         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_509546,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000563 DASVMB010000000
VERSION     DASVMB010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509546"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VF531_15760"
     CDS             complement(<1..319)
                     /locus_tag="VF531_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7715474.1"
                     /translation="MAGPVIFLSTCEFSGDMHGAVLIGKLRERLPEAKFYGIGGSKMA
                     AAGMDVLFDPTTRSTIGFIEALKNLRRMSRLLARVVQEWEVRRPDLMIWLDSGGFNLP
                     LALE"
     gene            complement(515..1270)
                     /locus_tag="VF531_15765"
     CDS             complement(515..1270)
                     /locus_tag="VF531_15765"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HEX7715475.1"
                     /translation="MKAWRGWPDRLRVWMSADNRAAWQPVLILGCFIILVLLLELVLN
                     LWCRAALRRAFSQDLRPETRLSTSMKWLSLADFGRGRIGWLKVRGQNCRIAELKYREL
                     TIDNRGLELDWPSLLRERRLRLKRLGRTRAHAVVAADALQSYLATLYPDLHPRLELAA
                     GRLRLGGTANLFGSPVQLELAASLSISSGKKVRLTPLGLWAAGREVAPGLVRFLTGQI
                     PLEFAIMQNWPLELTRLTLKPGALYLALKESGD"
     gene            complement(1572..2558)
                     /locus_tag="VF531_15770"
     CDS             complement(1572..2558)
                     /locus_tag="VF531_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7715476.1"
                     /translation="MKKRWILLLICGLLVASVSVGYSASKNVFAGNPKEEYYIVTFVS
                     GIEYWRGCFQGMKDAAGLLGVKAIYTGAPQSDVNQEVTVLEQVIAKKPAGIAVTCVNP
                     DGLKAPINKAIAAGIPVVTFDADSPASDRYCYLGTSNYNAGATAARYMGKLLGGQGEV
                     AVSTVTGQLNHEQRKAGFVETLAAEYANIKVVSTGNDNNDQTKAATLISGFLQAHPDL
                     KGVFCTDALGGVGAAAAIREAGAVGKVKIVSFDTDKGTLDLIKEGVISGSIAQGTWNM
                     GYWSMHFLYNVKHNLVKPVEGWRTKGINPLPGVVDTGTNVVTKTNVEAFYTK"
BASE COUNT          654 a          770 c          584 g          623 t
ORIGIN      
        1 cctcaagagc caaaggaaga ttaaaacctc cggaatccag ccagatcatc aggtctggac
       61 ggcgtacctc ccattcctgt acaaccctgg caagcaagcg actcatccgg cgcagatttt
      121 tcagcgcctc tataaatccg atggtgctac gagttgtagg atcaaagaga acatccattc
      181 ccgccgcggc catcttggaa ccgcctatgc cgtaaaattt cgcctcaggc aagcgctccc
      241 gaagctttcc aattaagacc gctccatgca tatctccgga gaattcacag gttgataaga
      301 agatcaccgg tccagccatt gcgtcccttt ctaacatcat ttatataacg attttatcgt
      361 gcattctctt tcaaaggtca atatttacca aacttattcg tcttaaagca cgaattacct
      421 ttttttattg gaaaaggcat gaggtataag gcgcgaggaa tgagtttcaa attgccttac
      481 tcatgcctct tgcctcgtgc ctcgtgcctg ttgcctaatc accagactct tttagcgcca
      541 aataaagcgc tccgggtttt aatgtcaacc gggtaagttc caggggccaa ttttgcatga
      601 ttgcgaattc cagcggaatc tgtccggtta aaaaacgtac caaacccggc gcaacttcac
      661 gcccggcggc ccatagtccc aaaggggtga gacgtacctt cttccctgag ctaatgctca
      721 gactggccgc gagttccagc tgaaccgggc taccgaaaag gttcgcggta cccccaagtc
      781 gaagtcggcc ggctgccagt tccagtcgcg gatggagatc cgggtaaagg gtggccagat
      841 aactttgaag agcatcggca gccaccacgg catgagcccg ggtccgaccg agccttttca
      901 atcttaagcg ccgttccctg agaagcgacg gccagtccaa ctcgagccca cggttgtcta
      961 tggttaactc gcgatatttc aactcggcaa tccggcagtt ttgaccccga actttaagcc
     1021 agccgatccg gccccttcca aaatccgcca ggctcaacca tttcatcgag gtactgagcc
     1081 gcgtctccgg tcggagatcc tggctaaacg ctctccgtaa cgcagcacgg caccagaggt
     1141 tgagcaccaa ctcaaggaga aggaccagta tgataaaaca acccaaaatc aacaccggtt
     1201 gccaggcagc ccggttatcc gcgctcatcc aaacccggag ccggtccggc catccacgcc
     1261 atgccttcat tctcatcccc ccgcttgttt cattgtacgc gaatcccgga ggattattcg
     1321 aaccgaggat ggattacagc agcgcctaaa tatctccaca aaaacaaggt tttccgatcc
     1381 cgtttttaac gggcttttga atgaattaaa acttaaccca gggtttctcg cgtcccttgg
     1441 gacgcgcacc ctgggcggac agaacgacaa tatgattttt ggggaaaacc ccggggctac
     1501 tcatcaattc cctttcttac ttaagccccc tgccgagtca tttcttggat catgattttt
     1561 acgaaccgca gttacttggt gtaaaatgcc tccacattgg tcttggtcac tacgttcgtg
     1621 ccggtatcga ccactccggg caatggattg atacctttgg tacgccagcc ttccaccggt
     1681 tttaccaggt tatgtttgac attatagagg aaatgcatcg accagtatcc catgttccag
     1741 gttccctggg cgatggaacc gctaatgaca ccctctttga tcagatccaa agtaccctta
     1801 tccgtatcaa agctgacgat tttaaccttg ccgacagcgc cagcctcacg gatggcggca
     1861 gccgcgccca ctccgcccag ggcatcggtg caaaatacac cttttaagtc gggatgggcc
     1921 tggaggaacc cggagatcaa agtggcggct ttcgtttgat cattattgtc attgccagtg
     1981 gaaacaacct taatattcgc atattccgca gccaaggtct caacaaaacc cgcctttctc
     2041 tgctcatggt tcaattgtcc ggtaaccgta gagacagcca cctctccttg accgccgagc
     2101 agtttgccca tatatcgggc agcagtcgcg cccgcgttat aattacttgt ccccaaatag
     2161 cagtaacggt cactggccgg agaatcggca tcaaaggtta caaccggaat ccccgcggca
     2221 atcgctttgt tgatcggggc tttcaatcca tccggattca cacaggttac tgcaatacca
     2281 gcaggctttt tggcgattac ctgctcaagc acggtgactt cctggttcac gtcggactga
     2341 ggagctccgg tatagatcgc tttgactccc aaaagacccg ccgcatcctt catcccttgg
     2401 aagcaccccc tccagtactc gataccggag acgaaggtta caatataata ctcttccttg
     2461 ggattacccg caaaaacatt cttgctggcg gagtacccca ccgacaccga ggcgaccaac
     2521 aatccacaaa tcaataacag aatccatctc tttttcatca acaacccccc gtaattcaat
     2581 tttctttacc atggaatcca ccgcgtacag aatcaaatag attcgaaggt c
//
LOCUS       DASVMB010000564         6715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_292404,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000564 DASVMB010000000
VERSION     DASVMB010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292404"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF531_15775"
     CDS             <1..225
                     /locus_tag="VF531_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715477.1"
                     /translation="GIHRRQDPGSPIIEDLWGHQQQILDMIGAIRDDRPPVITGEDGA
                     KSVELVLAIYESARKGGEPVGLPLAGNKSI"
     gene            314..1633
                     /locus_tag="VF531_15780"
     CDS             314..1633
                     /locus_tag="VF531_15780"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEX7715478.1"
                     /translation="MKETKLLAGFAKVNINPAFDCEMSGFVARKGNCRGVHDPLWVRS
                     LVLSDGKEKLVIAVADLIGLVHDTVQRIRTEVAALTDIPPEHVIVSTIHTHAGPATIE
                     HGYLGKPDPDYLNFLVKNIAGSIYAANQELEPVVASVGISECKAVGKNRRHQGGPTDP
                     AVSVLRLERAEERKILLANYTCHPVVLGPDNLLLSADYPYYLIKTLEAVYPGSEILFT
                     NGATGDINVGHNAADSINGSSASKRTFHEAERLGRILAGRTVEAVETAEKLPSIDLSF
                     ASRKFSLCLETVPDPSGYQILADQFAKEYQETSRRGESYGLVRQAEVWTDWAKAMKQK
                     AENGELDSKILTEVAAFRIGNVEFATLPGEFFHELGLMVKRSRPGKAVFIIGYANDLI
                     GYVGTSIEYDEGGYEINESYRYFGMPTKLARGAGERVIDEVKGLLQK"
     gene            1737..2519
                     /locus_tag="VF531_15785"
     CDS             1737..2519
                     /locus_tag="VF531_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7715479.1"
                     /translation="MQPKFIFSSRPSGGDYHAAVAFAQANHFDGIDWNLDCFRIAAAS
                     NARKVFCDTALNSGLPSRFHAPCHDVEIAHSNPNFAVSAFACIRMYIDFLKFFGPTYI
                     NLHIGSRSISEQELDWNTAVRNLKELVRYGKEQGVTVCLENLKKGWTSDPAKMAALVG
                     ESDAMVTLDVGHARAFLKASQSKLSLEEYVKPYADRIRNLHLYEIESVEGRHMPPDNL
                     GAIRDITRWALNHGIDWWVIELSGYDEALHTKALLEKEYFGR"
     gene            2625..3617
                     /locus_tag="VF531_15790"
     CDS             2625..3617
                     /locus_tag="VF531_15790"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7715480.1"
                     /translation="MKTRKWVFGLLSVLMIVTFVMMPVAMGAGRVVVYTPAPKNITDI
                     IVDMWNKENPNIPVDMISAGTGELVTRLRTESVNPQADILFGGGTEEVDGMLDLLQPY
                     KSVNDAAYKKGYKHPKYYYYGYSMPLQVFIINTTQVTEKTAPQTWKQLGDAKWKGKMI
                     MANPAVSSSGYAQVSIMLQRYGWDLVQKVIANATITSSSKLSYQGVADGEYPVGITGE
                     ANVFNLIDQGYPVKAIYPKDGTALRFDTMSIIKNCPDLENAKKFMDFLTSKPVMTAIA
                     QQAGVRVCRGDVQLKTGLIPNSKIKFLKYDAKLAADQKNANLAKFDEIFSAKKN"
     gene            3703..4794
                     /locus_tag="VF531_15795"
     CDS             3703..4794
                     /locus_tag="VF531_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715481.1"
                     /translation="MIDLSVKDLVKEFSGGTRAVDNINFTVPKGSLFTLLGPSGCGKT
                     TTLRMIAGLETPTSGQILFGNEDYTYFPPHRREIGMVFQSYALFPHMTVFDNVAYGLK
                     IRNVKKAEIRQRVEKVLDLVGLANTGDRKPSQMSGGQQQRIALARALVYDPKILLLDE
                     PLSNLDAKLRVYMRDEIRKIQKKAGITTVYVTHDQEEALSIADYIAVMHGGKISQFGK
                     SYDIYEHPASVLVADFIGKANFLTGEIVGKEKGMDQVSVCGSTIRVASGQGEVGKRAV
                     LFFRPEKAYLSSQIVSGKSIECVVSNILYLGESVCYTAKVNTGEEIKVSVNRRIENVD
                     EGSKAYLVLEPNDGILFPIEQLTLLNRGN"
     gene            4797..6518
                     /locus_tag="VF531_15800"
     CDS             4797..6518
                     /locus_tag="VF531_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7715482.1"
                     /translation="MSTGNLELNPIVKKRLTWRDIDFSVIIVMFLVIAVLIMFLVYPI
                     GKMLLSSFLEQGKPFALHNLTLVNFSKFFTSNLYVQATINSINTSVASVIVSILLGLP
                     LAYIMSRVAIPCKTLFTSLATLPIILPPFVGAYSWILLLGRNGFISFLLRQWFGITMP
                     SIYGFTGITLAMSLCYYPYVFLMAQGALSVADPYLEESAMVMGASFWRRLRTVIIPLV
                     LPSIAAGAITVFMRAIGNFGVPSILGGEYYTLPTLIFFQVAGYFNLNTASAISIVSIL
                     FSVLALLLMKYITARNAGVTMTTTTRAVKQINNPVVKTLSLVYVILLIVVSLAPHLTV
                     LLAGFSERWAGTPWPTKLSLLNFQKIFISAIRPLENSLFLAVVATLLSIFFGTLIAYT
                     TVKKKFKGRWLIDVMVMLPFILPGIVVGVAILAAFVKPPLYLAGTAYILIIAYFIRRM
                     PYVFRSAVGSLSGMDPAMEQASTIMGASWGATFRRVTFPLITPSILAAGLLTFTTLIG
                     ELSTTMILYSANWKTVTVSIYEYLLEDKMGPACALGTIVTVVVLAGIMIANKLLGEKI
                     GNMFRAG"
BASE COUNT         1758 a         1409 c         1729 g         1819 t
ORIGIN      
        1 ggtattcatc ggcgccagga tccggggagt ccaattattg aagacctgtg gggacatcaa
       61 caacaaatac tggatatgat cggagcgatc cgggatgacc ggccgccggt catcaccggt
      121 gaagacggtg ccaagtcggt ggaactggta ctcgcgatct atgaatcggc ccgtaagggc
      181 ggtgaaccgg tcggcctgcc tttggctggt aacaagtcga tttagaaagt gagtaaatct
      241 cgtcagctta tttttctttt gttcaggatt tggaatgagt gaatgagaaa gatgatgaat
      301 gaagggattc catatgaaag agacaaaact gctggcgggg ttcgccaaag ttaacattaa
      361 tcccgctttt gattgcgaaa tgtcgggttt tgtcgccaga aaagggaatt gccgcggcgt
      421 gcatgatcca ctttgggttc ggtcgctggt tctttcggac ggaaaagaaa agttggttat
      481 tgccgtggcg gatctaattg gtttggtcca tgatacggtt caacgaataa gaacagaagt
      541 tgccgccctc accgatattc cgccggaaca cgtcatcgtc agcacgattc atacccacgc
      601 cggcccggcg acgatcgagc atggctattt ggggaagccc gaccccgatt atttgaattt
      661 cctcgttaag aatatcgccg gttccattta tgcggccaat caagaactgg aaccggtcgt
      721 tgcctcggtc gggatcagtg agtgcaaggc ggttggtaaa aaccgacgtc atcagggggg
      781 accgactgat ccggcggttt cggtcttgcg attggagcgt gctgaagagc gcaagatatt
      841 gttggccaat tatacctgcc acccggtagt actgggaccg gataatctgt tgctttcagc
      901 cgattaccct tactacctga ttaaaactct ggaggcggtt tatccggggt cggagattct
      961 ctttaccaat ggtgcaaccg gtgatatcaa tgtgggtcat aatgccgcgg atagtattaa
     1021 tggttcatcc gcttccaaaa gaacctttca tgaggcggag cggctggggc gtattttagc
     1081 gggccggacc gtggaagcgg tagagacagc cgaaaaattg ccatctatcg atctttcctt
     1141 tgcaagtcga aagttctcat tatgtttgga aacagtgccg gatccatcgg gctatcagat
     1201 acttgcggat caatttgcca aagagtatca ggaaaccagt cgccgtggcg agagttacgg
     1261 cctcgtccgg caggcggagg tctggaccga ttgggcgaag gccatgaaac aaaaggcaga
     1321 aaacggggag ctggattcga aaatacttac cgaagtggcg gctttccgga ttgggaacgt
     1381 cgaatttgca accttacccg gtgaattctt tcatgaattg ggtttgatgg tgaagcgatc
     1441 gcgaccgggg aaagcggttt ttatcatcgg ttatgccaat gatctcatcg gttacgtggg
     1501 tacatccatt gaatatgacg agggaggcta tgagatcaac gagtcatacc gctattttgg
     1561 aatgccgacc aaattggcac gcggagcagg tgaacgggtc attgatgaag taaaaggctt
     1621 attacagaag tgatatgata agatctgctt aagctttttg agtaaaatga cgatttgtaa
     1681 tataaaaatg aaataaaaat gaaattttaa tttcatttga tacaaaggag atcaagatgc
     1741 agcctaaatt tatcttctca tcccggccaa gcggtggcga ttatcacgct gctgtagctt
     1801 ttgcacaggc gaatcatttt gacggaatcg attggaattt ggattgtttc cggattgcgg
     1861 ccgcatccaa tgccagaaaa gtattttgtg atacagcgct caacagcggt ttaccatcgc
     1921 ggtttcatgc tccctgtcat gatgtggaga ttgctcatag caacccgaat tttgccgtgt
     1981 cggcatttgc gtgtatacgg atgtatattg attttttaaa gtttttcggg cctacatata
     2041 tcaacttgca tatcggttcc agaagcatct ccgaacaaga attggattgg aacactgcgg
     2101 ttcgtaatct gaaagagctg gtccgttacg gaaaagaaca aggggtaacg gtttgtctgg
     2161 aaaatctgaa aaagggatgg accagtgacc cggccaaaat ggcggcattg gttggagaat
     2221 cggatgcgat ggttaccttg gatgtcggtc atgccagggc gtttttaaag gcatcccaaa
     2281 gcaagttaag tcttgaggag tatgtgaaac catatgccga tagaattcgc aatttacact
     2341 tatatgagat tgaatccgtt gaaggtcggc acatgccccc tgataatctc ggtgcaatcc
     2401 gcgacattac ccgctgggcg ttgaatcatg gcatcgactg gtgggtgatt gaattaagcg
     2461 gttatgacga ggcgcttcat accaaagcgt tactcgaaaa ggagtatttt gggaggtaaa
     2521 agtcccaagg tacggaggag gtgaagcgaa gcgggcaatt ggaacaggga ttcttgtttg
     2581 taacatgagt cgtaattgaa atcagcatca aaaggagaat gatcatgaag acaaggaaat
     2641 gggtatttgg cttgttaagc gtattgatga ttgttacctt cgtaatgatg ccggtagcca
     2701 tgggcgccgg cagagtcgtg gtctatacac cggctcccaa aaacatcacc gatattattg
     2761 tggatatgtg gaataaagaa aaccccaata ttccggtgga tatgattagt gccgggaccg
     2821 gagaattggt aacaaggttg cgcaccgaga gtgtcaatcc tcaggccgac atcctgtttg
     2881 gcggcgggac tgaagaggtt gacggtatgc ttgacttgct tcagccatac aaatccgtga
     2941 atgacgcggc ttacaagaaa gggtacaaac atccgaaata ttattattac gggtactcta
     3001 tgccgttaca ggtatttatc attaacacca cccaggtcac tgaaaagact gcgcctcaga
     3061 cttggaagca gttgggagat gccaaatgga aaggtaaaat gatcatggca aatccggcgg
     3121 tctcatcttc cggttatgct caagtcagca tcatgttgca acgttatggc tgggatttgg
     3181 ttcaaaaggt cattgccaat gcaacgatta cttcttcctc caaattatcc tatcagggcg
     3241 ttgccgatgg cgagtatccg gtaggcatca ccggggaagc caatgtattt aatttgatcg
     3301 atcaaggata tcctgtaaaa gccatttacc ccaaagatgg gacagccctg cgttttgata
     3361 ccatgtccat catcaaaaac tgccctgacc ttgaaaatgc caaaaaattc atggactttc
     3421 tcaccagtaa accggttatg accgctattg cccaacaggc aggcgtccgg gtctgccggg
     3481 gcgatgtcca gctgaagacc gggttgatac cgaacagcaa gattaaattc ctgaaatacg
     3541 atgccaaact cgccgcagat cagaagaatg cgaatctggc taaatttgac gagattttct
     3601 ccgcgaaaaa gaattaaatt cttctggatt ggatccaacc ggcgcgggaa tttagggata
     3661 aattcccgcg cctctaaaga aatgtgccga ggaggtaaat tcatgattga tttaagcgta
     3721 aaagatttgg tgaaagagtt ttccggtggc acccgagctg ttgacaacat taactttacc
     3781 gttcccaaag gcagtttatt tacacttctc ggcccgagcg gatgcggcaa gacaacaacc
     3841 ttacggatga ttgccggact ggaaacacct acatccggcc agattctgtt tgggaacgag
     3901 gattacacct attttcctcc gcatcggcga gagataggga tggtttttca aagttatgcg
     3961 ctttttccac atatgacggt ttttgataat gttgcttacg gattaaaaat ccgcaatgtc
     4021 aaaaaggctg agatacgcca acgcgttgaa aaagtcttgg atttggtggg attggcgaat
     4081 accggtgatc gaaagccgtc ccaaatgagc ggcggtcagc agcagcggat cgcactggca
     4141 cgggcattgg tatatgaccc caagatttta ttgctggatg agcccttaag taacttggat
     4201 gcaaaattgc gtgtgtatat gcgggatgag atccgcaaaa ttcagaaaaa ggccggcatt
     4261 acgacggttt atgtaaccca tgatcaggaa gaagccctca gtattgccga ttatattgca
     4321 gtgatgcatg gcgggaaaat aagccaattt ggcaagtcat atgatatcta tgaacatccg
     4381 gcttcagttt tagtcgccga tttcatcggc aaagccaact ttttaacagg agagatagtg
     4441 ggtaaagaga agggaatgga tcaagtgagc gtttgcggct caacgatccg ggtcgcttcc
     4501 ggccaggggg aagtgggaaa aagggcggtc ctgttcttcc ggcccgagaa ggcttattta
     4561 tcttcacaga ttgtttcagg aaaaagtatt gaatgtgtag tctctaatat tttatatctg
     4621 ggagaatcag tctgttatac tgccaaggta aacaccggcg aagagataaa agtatcggta
     4681 aaccgccgga tcgagaatgt ggatgagggt tcaaaggctt atctggtatt ggaaccgaat
     4741 gatggaatat tattcccgat tgaacagctg actctattga acagggggaa ttaacggtga
     4801 gtactggtaa ccttgaacta aacccgatcg tgaaaaaacg gttgacgtgg agggatatcg
     4861 atttttccgt catcatcgtt atgttcctcg taatagccgt tttaataatg tttcttgtct
     4921 atcctatcgg taaaatgctg ctctccagtt tcctggagca aggcaagcca tttgcattgc
     4981 ataacttgac cctggttaat ttcagcaagt tttttacatc caatctttat gtacaggcca
     5041 caatcaattc gatcaatacc agtgtggctt cggtaattgt ttcaatcttg ctcggactgc
     5101 cgctggccta tattatgagc cgggtcgcga ttccctgtaa gactctattt acttcactgg
     5161 ccactctgcc aattatcctg cctccctttg taggggctta ctcatggata ctattattgg
     5221 gacgtaacgg gtttatttcg tttctgcttc gtcagtggtt tggcattacc atgccgagta
     5281 tctacgggtt caccggaatc acactcgcaa tgtccttgtg ttactaccct tatgttttct
     5341 taatggccca aggcgccctc tccgtagccg atccttacct ggaagaaagc gccatggtga
     5401 tgggggccag tttctggcgc cgacttcgca cggtaatcat tcccttggta ctgccatcca
     5461 ttgccgccgg agcaatcact gttttcatga gagccatcgg caatttcggt gttccgtcga
     5521 ttttgggcgg cgaatattat accttaccga cgctgatctt tttccaagtg gcaggttatt
     5581 tcaatctcaa tacggccagc gccattagta tagtgagtat tttgttttcg gtcttagcct
     5641 tgttgttaat gaaatacatt accgcgcgga atgccggggt gaccatgacc acgaccactc
     5701 gggcggtaaa gcagatcaat aatccggtcg taaaaacact aagtctggtt tatgttattt
     5761 tgctcattgt agtatcgtta gccccgcacc taacggtatt gttggccggg ttttcagaac
     5821 gctgggccgg tactccgtgg cccacgaaat tatcgttgct caactttcaa aaaatcttta
     5881 ttagtgcaat acgtcccttg gaaaacagtc tgtttttggc agttgtggcc acattattgt
     5941 cgatattctt tggtacattg atcgcataca caacggttaa gaagaaattt aaaggccgtt
     6001 ggttgatcga tgtcatggtc atgctgcctt ttattttacc ggggatcgtt gttggtgtgg
     6061 caattttggc tgcctttgtc aaaccgcctt tatatctggc cggtactgcg tatattttga
     6121 ttatcgccta ttttatccgg cggatgccct atgtcttccg gtcggctgta ggatccctaa
     6181 gcggaatgga tccggccatg gaacaagcgt cgaccattat gggcgcaagt tggggagcta
     6241 ctttcaggcg cgtaactttt cccttaatca cgccaagtat tttagctgcc ggactgctta
     6301 ctttcactac tttaatcggc gaactcagca ccacaatgat tttgtactcg gctaactgga
     6361 agacggtcac agtctcaatt tatgagtatt tgttggaaga taagatggga ccggcttgcg
     6421 cgttaggtac cattgtaacc gtggttgtcc tggccggcat catgattgcc aataaattat
     6481 tgggagagaa aatcggtaac atgttccggg cgggatgaca gttgagcgaa aagttgaaat
     6541 aaactctcct gttcatttag gagtaaacga attgaagaga aagctccatc tcaattaaga
     6601 gattggagtt ttttcttttt tataacatgt taggcgggtg gttgttctga agcccacttg
     6661 aattctaaat acgcaatgac tacgccataa agcatcgaaa gcagtatgtg ggaaa
//
LOCUS       DASVMB010000565         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_145868,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000565 DASVMB010000000
VERSION     DASVMB010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145868"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VF531_15805"
     CDS             complement(<1..500)
                     /locus_tag="VF531_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715483.1"
                     /translation="MRITARTEDRIFNIINVILLSFLVIITLYPFINTLAVSLNNSLD
                     SVRGGIYLWPRKFTWVNYEKILIKPEVYMGALTSLIRTLVGPLLTVTSCFLVAYGISR
                     RDFVLRKFVTTAFVLTMYFSGGLIPYYFLIRGLHLINNFLVYVIPTMVNAFYILVIRS
                     YISELP"
     gene            complement(621..1571)
                     /locus_tag="VF531_15810"
     CDS             complement(621..1571)
                     /locus_tag="VF531_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7715484.1"
                     /translation="MPKAMELEAYETKRRSFFQKIANQKQLLLITVPFLLFIFIFNYL
                     PLWGWIMAFQNYKPALGISGSSWAGLDQFKMLFSDSEFFLGLRNTLAISCLKLVLNTL
                     TAITLAILLNEVRINFFKRTVQTISYLPHFVSWVVAANIVYTSLSPDGGIVNEILMSL
                     HLIKEPIMFMAQNNLFWGIAALSEVWKEVGWNAIIYLAAMTTIDPELYETANIDGAGR
                     IKRIFAITIPGIMSTIKILLVLNIGFLLNAGMQQIYLLQNSANIEVSQILEIYILNYG
                     LKMGRYSYATAAGIFNSLVSITLVLAANRISKRIDGEKLF"
     gene            complement(1694..3325)
                     /locus_tag="VF531_15815"
     CDS             complement(1694..3325)
                     /locus_tag="VF531_15815"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7715485.1"
                     /translation="MKTRKILGFLMLVMLVSIFTTCLVWGEDKQVKTFTLYFGGSGAR
                     PVDLFQTKIGKKITELTDVTMKIEYLVGSDEATKAGIMIASGDYPDLIYPHNVTHKFV
                     EAGALIPLDDYVAKSEVVKKAYKGNLKKLRWSDGHIYFLSPYQPDAERLYASDGFWLP
                     IDLLKENGWPMVTDIDQYFDLIRKYVKKHPSYNGKPTIGFTTQADDWRIYILKQAGIH
                     LAGYYNTGETIIDPKDNTAHTYALSPYTKTYLKKLNGLYLEGLFDKEAFSQNYDQYTA
                     KIAAGRVIGFYDERWQFLDPAKALEDQGLFGRMHVAMPVVYKGVKQNPYGGRQILSNI
                     SGVAISKSCKDPDAAWKFMERFLADDIQKLNYWGIEGDDYSLVNGRMVKNAAQIKMGA
                     DPDYLDKQGLTRFQFLFPRIAWGENCKYADGNFVSPNDSPEYFAYQYKPYEKDVLKQY
                     NKETFANFMSPPAKPLYGFVWDVPVEDGSPERIALQKCEDTSKKWLPKVVMAAKGQFE
                     KVWSQYVAEYNKIDTKLLEKHYTTAVQQRIKDWKE"
     gene            complement(3679..4722)
                     /locus_tag="VF531_15820"
     CDS             complement(3679..4722)
                     /locus_tag="VF531_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7715486.1"
                     /translation="MRRKVTAQDIADELGLSRNTVSKALNNRNSIADSTRKIVIQKAT
                     EMGYKRIKSVPSMDRDFGFKKTGNIVAITHEYYIDASYWSIVSRGAADALSQAGYNMI
                     FDYIKAVEEDSLIIPQNISADNVDGIIILGSLKADYIKELLKLKLPVVYVDAAMELFG
                     TEFMRDTVLMENEESIYQITKSLIAQGHSQLGFIGDIGFCKSYYERWLGFKRALEEAN
                     LNLYHQFCITSKTLHHYQQLEEIKEPIDGLIPMPTAFICANDRSAIFLTKILKDKGLR
                     IPLDIAISGFDDIFESTVVEPHLTTVRILKEELGQRAAEELLWRIQNPKRPIEVIHIA
                     TEAIYRESTIKVS"
     gene            complement(4965..>5107)
                     /locus_tag="VF531_15825"
     CDS             complement(4965..>5107)
                     /locus_tag="VF531_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX7715487.1"
                     /translation="VILTGGVTEAEAADRLLAERKADLIGVGRAILNDSGWARRAVES
                     MR"
BASE COUNT         1375 a         1171 c         1132 g         1429 t
ORIGIN      
        1 tccggcaatt cactgatgta actgcggatc actaaaatat aaaaggcatt caccatggtg
       61 ggaatgacat acaccaaaaa attattgatc aaatgcaatc ctctgatcaa aaaataatag
      121 ggaatcaacc cgccgctaaa atacatggtc aagacaaaag cggtggtgac gaacttccgt
      181 aagacaaaat ccctgcggct gattccatag gccaccagaa aacaggaggt taccgttagt
      241 aagggaccaa ccagcgtacg gatgagggaa gtcagggcgc ccatatatac ctcgggtttg
      301 atgaggattt tttcgtagtt gacccaggta aacttccgcg gccataaata aattccgcct
      361 ctgaccgaat ccagggagtt gttcaaagac acggccaggg tgttgataaa cggataaagg
      421 gtgatgatca ccaaaaaaga gagcagtata acattaatga tattgaagat ccggtcttcg
      481 gttcgcgcag ttattctcat ggttcaacct cccggtatga attgtcgcaa cgaagttcgg
      541 aatgacaatt tccgttccaa tggaccttaa attcgcctgt caagttgaca aagggattcc
      601 tttttcgcgc tcgccccaaa ttaaaagagc ttttcaccgt caatccgttt ggaaatccgg
      661 ttggccgcga gaaccagcgt gatcgagacc agcgaattga agattccggc agcggtggca
      721 taggagtaac ggcccatttt tagaccgtaa ttcagaatat aaatctccag gatctgggat
      781 acctcgatgt tggccgagtt ttgcagtaaa tagatctgtt gcattccggc atttaacaga
      841 aaaccaatat ttaagaccag caaaattttg atggtcgaca taatcccggg gatggtgatg
      901 gcaaagatcc gcttgatccg tcccgcccca tcgatgttgg cggtttcgta caactccggg
      961 tcgatggtgg tcatggccgc caaataaatg atggcgttcc atcccacttc tttccagacc
     1021 tccgataaag cggcgatccc ccagaataaa ttgttctggg ccataaacat gatgggttcc
     1081 ttaatcaaat ggaggctcat taaaatttca ttgacgatac cgccgtcggg ggagagcgac
     1141 gtgtaaacaa tattggccgc caccacccag gaaacaaagt ggggcagata ggagatggtt
     1201 tgcacggttc gcttaaaaaa gttgattcgt acttcgttta acaggatcgc caaggtaatg
     1261 gcggtcaaag tattaagcac cagttttagg caactgatgg ctaaagtgtt gcgcaagccc
     1321 aaaaagaact cggaatccga gaaaagcatt ttaaattgat ccagtccggc ccaggaagaa
     1381 ccggaaatcc ccaaagccgg cttatagttt tgaaaagcca tgatccagcc ccataacggc
     1441 agataattaa agataaaaat aaacaacaag aaaggaactg taatcagcaa caattgcttt
     1501 tgattcgcta tcttctggaa aaaagaacgg cgtttcgtct catacgcctc cagttccata
     1561 gcttttggca tacactcacc ggctttcgat tatcagtatc gggcaaatgt taaattgaat
     1621 attcgctcgg ccaacgcgtg aaatttgaag gttggaagga tgcggcaaag cgcatccttc
     1681 ccaataatga agattactct ttccagtcct tgatccgttg ttgaacggcg gttgtatagt
     1741 gcttctccaa taatttggta tcgattttat tgtactccgc cacatattga ctccaaacct
     1801 tctcaaattg cccttttgcg gccatgacca ctttcggaag ccacttcttt gatgtatctt
     1861 cgcatttctg caaggcgatg cgctcgggtg aaccgtcttc gaccggtaca tcccatacaa
     1921 agccatagag aggtttcgcc gggggggaca tgaagttggc gaaggtctct ttgttatatt
     1981 gtttcagaac atctttttca tagggcttat attggtaagc gaagtattcc ggtgaatcat
     2041 tgggggagac aaagttgccg tcggcatatt tgcagttttc accccaggca atccggggga
     2101 ataaaaattg gaaacgcgtt aaaccttgct tgtcgaggta gtcgggatca gcgcccattt
     2161 taatctgtgc ggcgtttttc accattcggc cgttcactaa gctgtaatcg tcgccctcaa
     2221 taccccagta gttcaacttt tgaatatcat ccgccaggaa tcgttccatg aatttccaag
     2281 ctgcatccgg atccttgcag ctcttcgaga tagcgacacc gctaatgttg ctgaggatct
     2341 gacggccccc gtaagggttc tgcttcacac ctttataaac caccggcatg gcgacgtgca
     2401 tccggccgaa caatccctgg tcttctaggg cctttgccgg gtccaggaac tgccagcgtt
     2461 catcgtagaa accgatgacc cgtccggcag cgatcttggc tgtatactga tcgtagtttt
     2521 gagagaaagc ttctttatca aacaggcctt ccaagtatag tccattcagc tttttcaaat
     2581 aagttttagt atagggggag agcgcgtaag tatgggcggt attgtctttg ggatcaataa
     2641 tcgtctcgcc ggtattgtag tatccggcca gatggatccc ggcctgcttc aggatataaa
     2701 tccgccaatc atcggcctga gtggtgaatc cgatggtcgg cttgccgtta tagctcgggt
     2761 gttttttcac gtacttccgg atcaggtcga aatattgatc tatatcggtc accatcggcc
     2821 agccgttttc tttcaacaaa tcgatgggga gccagaaacc gtcgctggcg tatagccgtt
     2881 ccgcatccgg ttggtaaggc gaaagaaaat aaatatgccc gtcgctccag cgcaatttct
     2941 tgagattgcc tttatacgcc ttttttacaa cttcactttt ggcgacataa tcatctaacg
     3001 gaatcaaagc gccggcttcg acgaatttat gagttacatt gtggggatag atcagatccg
     3061 gataatcccc ggaggcgatc atgatcccgg ctttggttgc ttcgtcgctg ccgacgaggt
     3121 actcgatttt catcgtcaca tcggtaagtt cggtgatttt ctttccaatc ttggtttgga
     3181 agagatcaac cggccgcgca ccggaacctc cgaaatatag ggtaaaagtt ttgacttgct
     3241 tatcttcacc ccataccaaa caggttgtaa agatactgac tagcatgacc aacattagaa
     3301 aacccaaaat ttttctcgtt ttcatgcaaa ctatcctccc tcttcctatt cttgagaaac
     3361 gtttagctgt ctgatagttt cctcctttca aaagtagtta caggcatcat gaagatccta
     3421 tgaaaatttt atcaaacata tgttacaatg tcaattaagg atgtaaatat cgtaattaat
     3481 gttacccaaa ttaatgtaac atttgtaact tgttatctga atattgcgcg cgcacgcgcg
     3541 cgttattgaa gaacaccgtc gtgaaccggc tcgcgccggt cgcgatacga tctccttgca
     3601 cactgccggg tatataacaa aaaaagacgt taatctaacg tcttgtacgg tttttcattt
     3661 atatctatgt ctgttggctt atgatacctt gatggtggat tccctataga ttgcctcagt
     3721 ggcgatatga attacttcaa ttggcctttt ggggttttga atgcgccata gtagttcttc
     3781 agctgcgcgc tgccccagct cttctttgag aattctgact gtagtcaggt gcggttcgac
     3841 tacagtggat tcgaagatat cgtcaaaacc ggatatcgct atatccaggg ggattcttaa
     3901 acctttatct ttaagtattt tggttagaaa aatggctgac cggtcgttag cacagataaa
     3961 agcagttggc attgggatca atccatcgat aggctcttta atttcctcta actgctggta
     4021 atgatgtaat gttttagaag taatgcagaa ttggtgatat agattcagat ttgcttcttc
     4081 aagggctctt ttaaacccaa gccatctctc ataatagctc ttacaaaacc caatatctcc
     4141 aataaaacca agttgagagt ggccttgggc gattaaggat ttggtaatct gataaatgct
     4201 ctcttcgttc tccatcagaa ccgtatcccg catgaattcg gtgccgaaca actccatggc
     4261 cgcatcgaca taaaccaccg ggagtttcaa cttcagcagt tccttaatat aatcggcctt
     4321 taatgatccc aggatgatta tcccatcgac attatctgcc gagatatttt gaggaataat
     4381 caatgaatct tcttcaacag ctttgatata atcaaaaatc atattgtaac ccgcttggct
     4441 taaggcgtcc gctgcacccc gggatacaat tgaccaatac gaggcatcaa tataatattc
     4501 atgagtgatg gctacaatat ttccggtttt cttaaatccg aaatccctgt ccatggaggg
     4561 cacggatttt attcttttat aacccatttc cgttgctttt tgaataacga tttttcgggt
     4621 cgagtcagca atactgttac ggttgtttaa agccttggag acggtattcc tggataagcc
     4681 gagttcatca gctatatctt gggcggtaac ttttctcctc ataaaatatc ctttcctgag
     4741 ttacacattt atcaggttgc ttactgttaa tatggtaaag gtaaaaatgg gaaaagtcaa
     4801 gtattgggca atttggttca tgggttaaaa cgcccggttt ctaagggaag agccgttttt
     4861 acatttttgt ttacagatgt gacatatttt taaggacttc attcatatcc aggagcggat
     4921 ggaatagttt gaagtatgcg gttagctctc cacaatgtag ggtgtcaccg catactctcc
     4981 accgcacgcc gcgcccaacc ggaatcgttc aaaatcgccc gtcccacccc gatcagatcg
     5041 gccttccttt ccgccaataa tctatcggcc gcctccgcct cggtaactcc cccggtcaaa
     5101 atcaccg
//
LOCUS       DASVMB010000566         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_215914,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000566 DASVMB010000000
VERSION     DASVMB010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215914"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VF531_15830"
     CDS             <1..578
                     /locus_tag="VF531_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715488.1"
                     /translation="VYGYDISGRVFDLDYRGEVSLSNGLTSPMLNYDTLAADSKDGEW
                     IPRVSVGFTKSFDYGEVQDRINVTNEFYYNQAGYDKNIFQRLADNGDANLKATYLTDY
                     YQAYQNSKYYWGLFSSVGKFIVPEMTLSCNSIMNLVDKSATVSAGISYVPALTDMEFD
                     FTINGALGGENTEATFMGSRLSVNLGTRISF"
     gene            781..1803
                     /locus_tag="VF531_15835"
     CDS             781..1803
                     /locus_tag="VF531_15835"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX7715489.1"
                     /translation="MKKSFYIMLLLILCILQLLNVQQNSFATELGDNDFLPFNNDDNL
                     KGYLNREGRFVIQPKFQEASDFYNGRAIVRYNNKIGYINYEGNFIIEPKFEFASNFTE
                     GYAPVKYQGQWGFIDQMGNWVIKPRFDSAFCFFNGIACVSYNYRYGFIDKTGKFVIRP
                     QYTDVHYMTIFKDKYVPVQYGNKSQTTWGYIDKSGKSFFGRYESAWDFVDGLALVKIN
                     KKYGFINEDGQFVIEPHFEDALTFSEGVAAVKVNGKWGYINKTGDFQIKPQFDEVVGF
                     SEGMAAVCLGNRWGFINIKGNVVMNPQFITPAWFKNGLARVYKSNGDGDYINKDGNFL
                     VGSNKK"
     gene            complement(1949..3142)
                     /locus_tag="VF531_15840"
     CDS             complement(1949..3142)
                     /locus_tag="VF531_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEX7715490.1"
                     /translation="MTTWKRNLWVCWFGAFVTMTGLSQITPVLPLYIEELGIRNTAAV
                     EAWSGIAFGITFIASAIFSPIWGRMADQYGRKPMLLRASLGMAVVIALMGLVGNVYQL
                     VGLRLLMGFVSGYVSSAITLVATQTPREFSGWALGTLSTGAAGGTLLGPLIGGYLTEI
                     MGIRAVFFAIGALLLVTFAATVILVREEFTPAHDRPLGIRAVWDLIPNTKLILAMFIT
                     TFILQLAMMSIEPIITVYIKQLTSHTRYIALISGIAFSAPGIASILTSPQLGKLSDRI
                     GHRKVILGSLIATGILFIPQAFVRSPWELVALRFLLGISSAGLLPAVNSLVNENVPET
                     VTGRIFGYNQSAQFLGSFGGAVLGGQIAAGFGITYVFFFTSALLLINALWVYTHVAPE
                     RRNKK"
     gene            3395..3802
                     /locus_tag="VF531_15845"
     CDS             3395..3802
                     /locus_tag="VF531_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7715491.1"
                     /translation="MKLKELTQLMDIPPEEHEIEFNGLTNDSKSVQPGNLFIAINGYK
                     QNGFLYLDEAIRNGASAALIEHGLTGPPGFPAIPVGNIRRVQGLTASRFFSEPSRRLR
                     VIGVTGTNGKTTVAHLIKHLLSSVGIPAGLIGN"
     gene            4098..5471
                     /locus_tag="VF531_15850"
     CDS             4098..5471
                     /locus_tag="VF531_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7715492.1"
                     /translation="MNDIKSVLEKQRDFFESGATRGMGFRIDSLKRLRKAIGEYEGEI
                     LDGLKQDLNKSSFESYETELGMVREELNYCIKHLSKWAKPRRVRTPFTQFPAASYVYP
                     EPYGCVLVMSPWNYPFQLTIAPLIGSIAAGNCTVLKPSAYSPHTSEAIAAMIGACFDQ
                     SYIAAVLGGREANQTLLDEKFDYIFFTGGVTVGKLVMESAARHLTPITLELGGKSPCI
                     VDREADIELAAKRITWGKFLNAGQTCVAPDYLLVHSGVKDELLGKMARYIRDFYGDDS
                     FGPESDLPRIVNEKHFHRLQGLMRQGKIVAGGQFDETTLQIAPTIIDGITWEDPIMQE
                     EIFGPLLPVLTYEDLAEVVRDVNSRPKPLALYFFSSNRRHQERILKSISFGGGCINDT
                     IVHLATSYMPFGGVGESGMGGYHGRASFTTFTHEKSILKKSNLVDIELRYPPYRNKLK
                     LLKKVFG"
     gene            complement(5483..5692)
                     /locus_tag="VF531_15855"
     CDS             complement(5483..5692)
                     /locus_tag="VF531_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715493.1"
                     /translation="MQHKKMLITGLKEAFGDRLRTSGENAGLHLMVEFLDCSFTPPDL
                     ERLKNMRVIVDWVEDYASCLLCVSL"
     gene            5766..>6565
                     /locus_tag="VF531_15860"
     CDS             5766..>6565
                     /locus_tag="VF531_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7715494.1"
                     /translation="MQYRPFGKSGFQVSALGFGAMRLPLEGGKIDEAQATRMVRYAID
                     RGVNYVDTAYPYHDGASEVFLGSALKDGYRDKVKLTTKMPSWLIQTAADFDKYLDEQM
                     KRLQVDHLDFYLLHSLDGERWPKLRDLGVLEWAEKAIAAGRFSHLGFSFHHGPEVFRS
                     IVDGYDHWALAQIQYNYMDVENQAGIAGLKYAASKGIPVVIMEPLLGGKLTAPPRPVQ
                     AIWDSAAQKRTPVGWALAWLWDQPEVATVLSGMSSMEQVEENVALAEKS"
BASE COUNT         1755 a         1438 c         1691 g         1681 t
ORIGIN      
        1 cggtatacgg ctacgacatc tccggccggg tctttgacct cgattaccgt ggcgaggtca
       61 gtctttccaa tgggctcact tccccgatgt tgaattatga taccctggcg gcagatagta
      121 aagacgggga atggatcccg cgggttagtg taggattcac caaatccttt gactatgggg
      181 aagtccaaga tcgtatcaat gtaaccaacg aattttatta taaccaagcc ggttatgaca
      241 aaaatatttt tcaacgcctt gccgataacg gtgatgccaa tttgaaagct acgtatctga
      301 ccgattatta tcaagcctac cagaacagca aatactattg gggcctcttc agttcggtcg
      361 gcaaatttat cgtccccgag atgacgttga gttgcaacag catcatgaac ctggtggata
      421 aatcggctac tgtctccgcg ggaatcagtt atgttcccgc ccttaccgat atggaattcg
      481 actttaccat caacggcgct ctcggtggtg agaatactga agccacattt atggggagtc
      541 gcctcagcgt caatctgggc accaggattt cgttttagga aaaccgcaat cgatttgacc
      601 ggttggaatc cggagaaatt gaagattaac aacaaaggct gttgacattt ggtcgacagc
      661 ttttgttgtt agagagttcg gttttatatt aatctattga ctcaatagag gtttttaatt
      721 tttttagaag aaatatttca taaatataaa agtagagact tagcgagggg ggagtaccag
      781 atgaaaaaat cattctatat tatgttgtta ctgattttat gtatccttca gttattaaat
      841 gtccaacaga atagttttgc tacagaatta ggtgataatg atttccttcc ttttaataac
      901 gatgacaatt taaaaggata tcttaatagg gaaggtaggt ttgtaattca gccaaaattt
      961 caggaggcat cggattttta taatggtcgc gcaattgtca gatacaataa taagatagga
     1021 tatataaact atgagggtaa ttttattatc gaacctaaat ttgagtttgc aagtaatttc
     1081 actgaagggt atgcacctgt gaagtatcag ggacaatggg gattcattga tcaaatggga
     1141 aattgggtaa ttaaacctag atttgattcg gcattctgct tttttaatgg gattgcttgt
     1201 gtatcatata attatcgtta cggtttcatt gacaagaccg gaaaatttgt tataagacct
     1261 caatatacag atgtgcatta tatgacaatt tttaaagata aatacgttcc agtccaatat
     1321 ggcaataaaa gtcaaaccac ctggggatat attgataaat cgggaaaaag tttttttggc
     1381 cgttatgaat cggcatggga ttttgtagat ggattggcgc ttgtaaagat caataagaaa
     1441 tacggattta tcaatgaaga cggtcaattt gtaatagaac cccattttga agatgcacta
     1501 acgttttcag aaggtgtagc agcggttaaa gtcaacggaa aatggggata tatcaataaa
     1561 acaggagatt ttcagatcaa gccgcaattt gatgaagtgg tcgggttttc agagggtatg
     1621 gctgctgttt gtttaggaaa ccgatgggga tttattaata taaaaggtaa tgtcgtaatg
     1681 aatccgcaat ttatcactcc cgcatggttt aaaaatggat tggcacgtgt atataaatcc
     1741 aatggtgatg gggattatat caataaagat ggtaactttt tagttggctc aaataaaaag
     1801 tgagacgaaa aattagtaag ttatcttgat agagaatttg attataagaa attttcatcc
     1861 gtataccata aatatgggac cgtgttaata gatcgatttt atcgagatat cgagagtggt
     1921 attagttatc gtaatgccac tcgttttttt attttttgtt tcttctttcc ggcgctacat
     1981 gggtatacac ccacaatgca ttaatgagta atagggcgct cgtgaagaag aaaacataag
     2041 taatgccgaa accggccgcg atttgccctc ccagcaccgc accgccgaaa gagcctaaga
     2101 actgggccga ttgattgtag ccgaagattc tcccggtgac cgtctccggc acgttctcat
     2161 tgaccagact attaactgcc ggtaataatc cggccgagga gatccccaat aaaaaccgga
     2221 gcgccaccag ttcccagggt gatcgcacaa aagcttgggg tataaataag attcccgtag
     2281 cgatcaagga ccccaggatt actttgcggt ggccgatgcg atctgaaagt tttcccagtt
     2341 gcggcgaggt caggatactg gcgatccctg gagcggagaa ggcgattccg gaaattaatg
     2401 cgatatagcg tgtatgagag gtcagctgct tgatatagac cgtaataatt ggttcaatcg
     2461 acatcatcgc cagttgcagg atgaatgtgg tgataaacat ggcgaggatt agtttggtgt
     2521 tagggatgag gtcccaaacg gcgcggatcc cgaggggcct atcatgggcg ggggtaaact
     2581 cctcccggac caaaatgacc gtggccgcga aggtcactaa aagcaatgcg ccgatggcaa
     2641 aaaagacggc tcggattccc atgatctccg taagataacc gccgatcagc ggaccgagca
     2701 gcgtgccgcc ggccgctccg gtggaaaggg ttcccagagc ccatcccgag aattcccggg
     2761 gtgtctgcgt agccaccagg gtgatggcgg aagagacata tccggagaca aaccccataa
     2821 gtaatcgcaa tcctacgagt tgataaacat tgcccaccaa gcccattaag gcgatgacga
     2881 cagccattcc cagactcgcc cggagcagca tgggcttgcg tccgtattgg tcggccatcc
     2941 gcccccagat cggtgagaaa atggccgaag cgataaaggt gatcccaaag gcaattcccg
     3001 accaggcttc gacggcggct gtgttacgaa tgccgagttc ctcgatgtaa agcggcagga
     3061 caggggtgat ctggcttaat ccggtcatgg tgacgaatgc cccgaaccaa cagacccaca
     3121 ggtttctttt ccaagttgtc atgtaacgtc cttcgctaaa gcaatttggt gtctaaactt
     3181 tatgtaactt tcattctata tatcctgtct ttttcctttc acctgaattc gacaagtcat
     3241 tgaaaattaa tacgttgcct ctctccggat aaatcggaaa aaaggtaaaa tgggacttcg
     3301 gcatacggac ttttatacta gtggtgccat atttatagca tcgaatcagg gtaacaccgg
     3361 aagggccatt gttatggaat gatggggtgt tggcatgaag ttgaaagaat taactcaact
     3421 tatggatatt cctccggaag aacatgagat cgagtttaac ggcttaacaa acgactcgaa
     3481 atctgtccaa ccggggaacc tatttatcgc gatcaatgga tataaacaga atggattttt
     3541 atacttggat gaggccattc gtaacggcgc cagtgctgcg ttgatagaac acggattaac
     3601 cggtcctccc ggttttccgg caataccggt tgggaatatt cgaagagttc aagggttgac
     3661 ggcctcgcga ttttttagtg agccatcccg tagattacgc gtcatcggag taaccggcac
     3721 caatggtaaa acaacagtcg cccatttgat caaacattta ttgagttcgg tgggtattcc
     3781 ggcggggctc atcggaaatt aggttcttcc ttggctgcgt acgaaaagaa tcatgccgga
     3841 taaagcgtgc gcttcgcgca cgagattcat ttcccgaatt gggggtttag gattcaaaga
     3901 tcgggggtgt tccttttcaa ccccggtttt ttcgatgtct gcttatatct tcggggcaaa
     3961 agcccaacta tgagagatac gtttccttgc gggcttacga tctttcgtta taggcaggat
     4021 aaccggaaga aaaggcgaat ttatttccag atttttaaat aaacaatttg tagcgcaagg
     4081 ctaagggagg accaccaatg aatgatatca aatcggtcct ggagaaacaa cgggattttt
     4141 ttgagagtgg cgccacccgg gggatgggct tccggattga ctctctgaaa cgactgagaa
     4201 aggcgattgg cgagtatgaa ggggagattt tggatggtct gaagcaggat ctgaataagt
     4261 cgtcctttga gtcctatgag accgaattgg gaatggtccg ggaagaattg aattactgta
     4321 taaagcatct atcgaaatgg gccaagcccc gccgggtgag gacgcctttc acccagtttc
     4381 ccgcggccag ttacgtatac cctgaaccct atggctgtgt tctggtcatg tcgccctgga
     4441 attatccttt ccagctgact atcgccccgc tgattggctc catcgccgcg ggtaactgta
     4501 cggttctcaa accgtccgcc tattcgcccc atacctcgga ggcaattgcc gcgatgatcg
     4561 gcgcgtgttt cgatcaatcc tatatcgcag cggtccttgg cggcagggag gccaaccaaa
     4621 cgctgctcga tgagaagttt gactacatct tttttacggg cggagtgacc gtcggcaagc
     4681 tcgtcatgga atcggcggcc cggcacctga cccccatcac cctggaacta ggcgggaaaa
     4741 gcccgtgcat cgtcgaccgg gaggccgata ttgaactggc cgccaagcga attacctggg
     4801 gaaagtttct caatgccgga cagacctgtg ttgccccgga ttacttactg gttcactccg
     4861 gcgtcaagga cgagttgctg ggcaagatgg cccgttatat ccgggacttt tacggggacg
     4921 attccttcgg gccggagtcc gatctgccgc ggatcgtcaa cgagaaacac ttccaccggc
     4981 tgcagggttt gatgcggcag gggaagatcg ttgccggcgg tcaatttgat gaaacaacct
     5041 tgcagattgc gccgacgatc atcgacggaa ttacctggga agacccgatc atgcaggaag
     5101 agatcttcgg gccgctctta ccggttctta cttatgaaga tttagcggag gttgtgcgcg
     5161 atgtaaacag caggcccaag ccgttggcgc tctatttctt ctcgagcaac cgccgccatc
     5221 aggagcggat tctaaagagc atctcctttg gaggcggctg catcaacgac accatcgttc
     5281 acttggctac ttcgtacatg cccttcggcg gagtcgggga gagcggcatg ggcggttatc
     5341 acggccgggc gagtttcacc acctttaccc atgaaaaaag catcctgaag aagtccaacc
     5401 tggtggatat cgaactacgg tatcccccgt accggaacaa gctgaaactg ctcaagaaag
     5461 ttttcggcta agcccatttt atttacaacg aaacacacaa aagacaggaa gcataatcct
     5521 ctacccaatc cacgatgacc ctcatgtttt ttagcctttc aagatcgggc ggcgtaaagg
     5581 aacaatctaa aaattccacc atcaggtgaa gcccggcgtt ttcgccgctc gtccggagcc
     5641 tgtctccgaa agcttccttt agccctgtga tcagcatttt tttgtgttgc attcacgaat
     5701 cgatcaaccc gtaaaagttg aagtgagtag ggaattcgat gaaatagaaa tggaggaaga
     5761 taacgatgca ataccggcct ttcggaaaat cagggtttca ggtttccgcc cttggtttcg
     5821 gggcgatgcg gcttccgctt gaaggtggca agatcgatga agcgcaagca accaggatgg
     5881 tgcgttacgc cattgaccgg ggtgtgaatt atgttgatac ggcctatcca tatcacgacg
     5941 gcgccagtga agtttttctc ggaagcgcac taaaagacgg ttaccgggat aaggtcaagc
     6001 ttacgaccaa gatgccgagc tggctaattc agaccgcggc agatttcgat aaatatcttg
     6061 atgagcagat gaaaaggtta caagtggatc atctggattt ttacctgctt cattctttgg
     6121 acggtgaaag atggcccaaa ctccgtgatc taggggtgct tgaatgggcg gaaaaggcca
     6181 ttgcggccgg gcgcttcagc catctggggt tctccttcca ccatggtccg gaggtattcc
     6241 gctcgatcgt cgacggctat gaccattggg cgctcgctca aatccagtat aactatatgg
     6301 acgtggagaa tcaagccgga attgcgggtt taaagtacgc ggcctcaaaa ggaattcctg
     6361 tggtcattat ggaaccgctg ctgggcggca agttaactgc tccgccccgg ccggtccagg
     6421 caatctggga cagcgccgct caaaagcgta cgccggtagg gtgggccctg gcgtggctct
     6481 gggatcaacc cgaggttgca acggtgctca gtgggatgag ctcgatggag caggtggagg
     6541 agaatgtagc ccttgccgaa aagtc
//
LOCUS       DASVMB010000567         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_145933,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000567 DASVMB010000000
VERSION     DASVMB010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145933"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VF531_15865"
     CDS             <1..681
                     /locus_tag="VF531_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7715495.1"
                     /translation="AIAGVDGNGQTELIEILTGLRQASAGHFFLENRELTNRTPKEVI
                     EAGIAHIPEDRQKRGLVLDFSIWENLILESYRDVDFSQSGFLKQKKALSYADVLIREF
                     DIRTPNSTVPMASLSGGNQQKAVVAREIRRNPKALIVAQPTRGLDVGAIEFVHKQIVA
                     ERDRGTAILLFSLELDEILALADRIAVIYEGEIVGILPREAATEEQLGLMMTGGLRFG
                     SNKGAVNQ"
     gene            678..1781
                     /locus_tag="VF531_15870"
     CDS             678..1781
                     /locus_tag="VF531_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715496.1"
                     /translation="MTASAIWEALKKYLGKLVFPFITLFFALFVGFFIISWLGSNPIV
                     AYWHLINGAFGSATKFGETLVYVSPLILTGLSIAVAFRCGLFNIGAEGQYVMGMIAAA
                     WIGAAFTNLPAWIHIPLTMLSGVLAGCIWGGIPGFFKAKYGAHEVITTIMMNYMALHF
                     TGYLVNHPLIAPPGTSPVTRTISESAQLTRFLPPSRVNTGIFVALFCILLIYLLLWKT
                     KWGFEIRAVGLNNEASRYAGISVTKNMVLAMAISGGLAGLAGALQIQGIQYRFNDLFG
                     FPGYGFDGIAVALLGNNHPVGVLFGALLFGAMNSGALQMQSVAGIPKDLIGVIQALII
                     FFVSADYALKKFHLPKIGHRTKTAAPATTGKGV"
     gene            1785..>2063
                     /locus_tag="VF531_15875"
     CDS             1785..>2063
                     /locus_tag="VF531_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715497.1"
                     /translation="MDLSFVLGIIAFVIASTLRAATPLTFAALGGVFSERSGVVNIGL
                     EGILLFGAFFGVYGSYLTGNPWIGVLFGAFFGGLTALIHAFVSIEFQAN"
BASE COUNT          444 a          518 c          546 g          555 t
ORIGIN      
        1 gctatagccg gtgtcgacgg caacggtcag acggagctga ttgaaatttt aaccggacta
       61 agacaggcat cggcaggtca ttttttcctc gaaaaccggg aactgaccaa ccggactccg
      121 aaagaggtta tcgaagccgg cattgcccat atcccggagg atcgacagaa acgtggtttg
      181 gtgctggact tttcgatatg ggaaaattta attcttgaga gttaccggga tgttgatttt
      241 tcccaatcgg gatttctcaa acaaaagaag gccttgagtt atgccgatgt gttgatccgg
      301 gaattcgata tccggactcc aaactctaca gtccctatgg catcactctc cggaggcaat
      361 caacaaaaag cagtagtcgc ccgggagatt cgccgtaatc ccaaggcgtt aatcgtagcc
      421 cagcccacac gggggttgga tgtaggagcc atcgaattcg ttcataagca gatcgttgcc
      481 gaacgcgatc ggggcacggc catcctcttg ttctcgctgg aattggatga gattttagct
      541 ttggccgatc ggatcgctgt aatctatgaa ggtgagatcg tcggaattct cccccgtgaa
      601 gccgccaccg aggaacagct cggcctgatg atgaccggcg gactacggtt cggttcaaat
      661 aagggggcgg ttaaccaatg actgcttcag cgatttggga agcactcaaa aagtacctgg
      721 gtaaattggt ttttcctttt atcactttgt tcttcgcgtt gtttgtcggg ttcttcatca
      781 tttcctggtt gggatcgaac cctatcgttg cctattggca cctgattaac ggggcgttcg
      841 gcagtgcaac caagttcggt gaaacccttg tctatgtctc accgttaatt cttaccggtt
      901 tgagcatcgc tgtcgctttc cgttgcggcc tgttcaacat cggagccgaa ggtcagtatg
      961 tcatgggaat gatcgccgcc gcctggattg gagcagcctt taccaaccta ccggcctgga
     1021 ttcatatacc tctaaccatg ctttccggag ttttagccgg atgcatctgg ggtgggattc
     1081 ccggtttctt taaggccaaa tacggagcgc atgaggtcat caccaccatt atgatgaatt
     1141 acatggcgct gcactttacc ggatatctgg taaatcatcc gttaatcgcc ccaccgggca
     1201 cctcgccggt aacccggacc atcagcgagt cggcccaact aacccgattt ctgccccctt
     1261 cacgggtcaa taccggaatc ttcgtggcat tattctgtat tctcttgata tacctgttat
     1321 tatggaaaac caagtgggga tttgaaatcc gggcggtggg tttgaataat gaagcctccc
     1381 ggtatgccgg gatcagcgtt accaaaaata tggtgctggc gatggcaatc agcggcggct
     1441 tggcgggtct ggccggagcg ttgcagattc agggaatcca gtatcgtttc aatgaccttt
     1501 ttggcttccc cggttatggc tttgacggaa ttgccgtggc attacttggc aacaaccatc
     1561 cggtgggggt gcttttcgga gcgctgcttt ttggcgccat gaacagcggc gcccttcaaa
     1621 tgcagagcgt tgccgggatt cccaaagatt tgatcggagt cattcaggcc ctcatcatct
     1681 tctttgtctc ggcggattac gccttgaaaa agttccatct gcccaagatc ggtcatcgta
     1741 cgaaaacagc cgcaccggcc acaacaggaa agggggtttg aacgatggat ttaagtttcg
     1801 tccttggaat cattgccttc gttatcgcct ccaccttacg ggcggctacc ccgctgactt
     1861 ttgccgcttt gggcggggtt ttctctgaaa ggtccggggt ggtcaatatt ggcctggaag
     1921 gcatcttact cttcggagcc ttttttggcg tctatggttc ttatctgaca ggtaaccctt
     1981 ggatcggagt acttttcggc gccttcttcg gcgggctgac tgccttgatt catgccttcg
     2041 tcagtattga atttcaagcc aac
//
LOCUS       DASVMB010000568        12782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_509973,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000568 DASVMB010000000
VERSION     DASVMB010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509973"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VF531_15880"
     CDS             <1..1668
                     /locus_tag="VF531_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7715498.1"
                     /translation="MTLGPGPGSFLEKFNPLTGQVIWRQNIAISGGINCGRYNRADRC
                     TYLVDGNGVLKFDPQGRQLGSILDLKQQSILGFETVVTGINVAKGAQYPLIYLINLNQ
                     QVRNLYNSGQPVAANGKPYNIYQYSYVPKLPKERNQKVITISTLMPDRFLETAISAYQ
                     SKHPGIKIELKSFVNGNIITKVEMEQFVKSVSAELMAGKGPDILSLGSSPFQKLADRN
                     VLVDLGALMAKDKSFDRNKYYSNILDACKYKDGLSVLPVFIQFDALELNPEILDKAGV
                     RIDDTRWNWDEFLKVAEKVSLDINGDGRPEQFALPNMDRLRLFNWMFETQYSSFIDRE
                     KKKAYFDTPEFIGLLNLCKTFSDKQLMSTQADPNASQFKIIINGQLMDKGAISTVAFT
                     PTTVFGYSSLLGAKTTFHTARLLSLPGNGAGSGFETPLGMLFGINRKSKLQKEAWEFL
                     KYLLSDEAQTFPGAMGFAVNKDAQRKRAAAEIDRETAQEIANPLNESDMEQVDRLITN
                     LRQLNNNFDPQMIAIIDDELRPFFSGQKSASEVAKLIQSKATTYLQE"
     gene            complement(1907..2161)
                     /locus_tag="VF531_15885"
     CDS             complement(1907..2161)
                     /locus_tag="VF531_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7715499.1"
                     /translation="MRTTGIVRKVDELGRIVIPKETREQLYIAVKDPLEIFIDEEQPS
                     IILKKYTPGCIFCGEMNGVKNFKDKMVCADCLAAIKRETK"
     gene            2389..4671
                     /locus_tag="VF531_15890"
     CDS             2389..4671
                     /locus_tag="VF531_15890"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7715500.1"
                     /translation="MLQFSAAIREGIDQIFRNAAADGRRMLYEHEVYGVLRLLDLAVP
                     EFILIEHVAELTEEQLKPFGREVVVKIVSPWIAHKQKVGGVKVVSNTGALFIQFVMTR
                     MKEEVLGHFPATDPPVIKGFLIEEYIDFSQALGYETLIGIKEDPAFGPVLTLSKGGDD
                     AEFFARYYDPANSFLPPLDSVTALRMLQTLHIRHKFEAIGHPEYLDYMARAVVNLSAL
                     ATAYSRVDTATPQYIIETFEVNPFVITRDHRLVALDGYAQFEPASGVDDRVTGLALTN
                     LDSFFTPRGIAVVGVSADPSKYSLGREIAMLLHDLNRTDLFLVNPKGGSLELNGTTFP
                     LYESPALLPQPVDLMVYTAPAQYMLDFIRQLADEPDTRRPRGLIMIPGIPADLDYAAF
                     AQRLATLVPAGLRIIGPNCMGVYFAPDQSNPGVNTLFIREQRLELKSSQFSNTVLLTQ
                     SGAFSVTAIDKLRNARVFQAIVSFGNKFDVKITDLMSYFAASPRIDVIALYIEGLDRG
                     EGRRFFELAGTIPKPIIVYKAGRTLAGARAAASHTASMTGSYEVFGAACRQAGVILAE
                     NLEDHYNYIRTFSLLSRRPPRGNRVAGVVNAGFESTVGADELKNLAQAKLGPETVVRL
                     NRINRFGLVDTTAPFLDITPMADDRMYAEFVEAILEDANVDCVFVAIVPHTPSLKTGP
                     EVCHDPDGLAWQLVELYRKYPKPLVVSVNAGRYYQEFVSVMEENGIPVFTDIRSTINS
                     LDQFVRYHVKGKNLNLGEEK"
     gene            4803..6317
                     /locus_tag="VF531_15895"
     CDS             4803..6317
                     /locus_tag="VF531_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033983.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX7715501.1"
                     /translation="MNMLLLFRWLAVAAFACLMVGIGLYALRRTKTSSDFFLAGRNVG
                     PWVSAFAYGTTYFSAVLFIGYAGSQGWGFGISVLWVALGNALIGSWLAWKVLARRTRS
                     ITARLGAMTMPEFLETRYQSKGLKIAAAILIFVFLIPYCGSVFTGLGFLFENVLNINY
                     QLSLVVMIVLTGIYLILGGYFAIAFNDFIQGLIMLVGAVLMVGYVFHQPQVGGFGGFI
                     PGLARIDPGLTQVIPTNWLGLLSLVILTSLGTWGMPQMVQKFYAIKNEQVIRTATIVA
                     TLFALVIAGAAYLVGSTVHLFFNAVPVDAAGNPVYNTLIPQLLNQVLPEALLIIILLL
                     VLSASMSTLSSLVLVSSSALTIDLIQGVFHPGIDKKHSLTLLRLFCGLFILCSIGVAV
                     FKIDFIIQLMALSWGTIAGAFLAPYLYGLFWKGTTKSGAWAGLISGIVISIGGSLVFP
                     DKNTAPLISAVAMLVPLGVVPLVSVFTKKYDAAHLERVFGPAEPPLTLAASTRE"
     gene            6873..7337
                     /locus_tag="VF531_15900"
     CDS             6873..7337
                     /locus_tag="VF531_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7715502.1"
                     /translation="MRLLPVGLQIRPMAEEDYRDVYRLYRQLVGTGLTYRATATQEQQ
                     EFSRIFQNFFQNPEREAYVAAVYDNVIGFVTLYYLDVFHHSGQIANIQELVVTEEFRG
                     RGVGKALVDFVKNKALEKHCRGLEVATDLWQSGAKSFYNKCGLHGKVLMMAT"
     gene            7639..9678
                     /gene="ligA"
                     /locus_tag="VF531_15905"
     CDS             7639..9678
                     /gene="ligA"
                     /locus_tag="VF531_15905"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7715503.1"
                     /translation="MTHSDLVPDDIRTKVARLRQTIEHHSYQYYVLDSPEIDDRQYDA
                     LIHELAALEAEYPELRTPDSPTVRVGGKPLELFETVRHQVPLLSLDNAYSAEELTEFD
                     KRVKRALGSDAELEYVAELKIDGLTVALTYERGLLIRGATRGNGEEGEDVTINVRTIK
                     SIPLRLPSSLSGVSLLGVRGEIYLKKADFLALNEQREIREEPLFANPRNAAAGSLRQL
                     DPKITAGRALDAFFYDILFLEGRQVPSQWDGFELMRAARLKTNPNSKRCQGIGQVIEY
                     CNYWTEHRGELPYEIDGIVVKVNDLRLQGQLGFTAKAPRSKTAFKFPAEQVETKVLDI
                     QVNVGRTGAVTPVAILESVRVAGSTVSRATLHNEDNVKEKDIRVGDRVIIQKAGDVIP
                     EVVRSLPEKRNGSERIFTMPVVCPECGSEVYREPGEAVARCIGATCPAQLRQGIIHFV
                     SREAMDIEGLGEAIVIQLIDSGLVKDVADLYELRYQDLIEQERFGAKSAANLLQAIEE
                     SKKNDLSQLLFGLGIRHVGAGAARELASRYGSLDRLMQASYDELTAIATIGPKIAGSV
                     IKYFEEPHNREVIVKLQRLEVDPRESGAGDTPQTLEGKTLVVTGTLESFGRTEVEEII
                     RRHGGKSASSVSKRTDYVLAGADPGSKLEKARSLGVRVIDEAEFKALLEGGSSNR"
     gene            9984..10304
                     /gene="gatC"
                     /locus_tag="VF531_15910"
     CDS             9984..10304
                     /gene="gatC"
                     /locus_tag="VF531_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7715504.1"
                     /translation="MGSEMHDQKKTTLSSEDVRHVARLARLELNSAEEEQFTKELNDI
                     LRFVEQLNELDTAGIEPTAHAITVRNVFRPDRAGVSLDPEAALANAPDRSGNFFKVPK
                     ILED"
     gene            10347..11810
                     /gene="gatA"
                     /locus_tag="VF531_15915"
     CDS             10347..11810
                     /gene="gatA"
                     /locus_tag="VF531_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7715505.1"
                     /translation="MELYQKTAHELHDLIRRKEVRAVEVVASVFDRIRAVESRINSYI
                     TLTEEQAVHTAKQVDMMVEKGELLPKLAGIPVALKDNLSTRGIPTSCGSKMLTDYIPP
                     YDAKVVELLAAGKMPLVGKTNLDEFAMGSSTENSAFHITRNPWDPDSVPGGSSGGSAA
                     ALSAGETILALGSDTGGSIRQPAGYCGIVGMKPTYGRVSRYGLVAFASSLDQIGPMAK
                     DVTDCALLLQAIAEYDPRDSTCVNHPVPDYLREMQRGVKGMRIGLAQEYLGEGISTSV
                     RSAMDEAVKVFRDLGAEIVPITLPHTRYALATYYVLAPAEASSNLARYDGLRYGHRTS
                     AQVSDSVEMFMKSRSEGFGPEVKRRIMIGTYVLSAGYYDAYYKKAQQVRTLLRDDYNK
                     AFSGCDLILTPTTPSVAFKIGSKTQDPLTMYLEDICTITCNLVGIPGISIPCGFDHGL
                     PIGMQLLGPAFGEGSLLRAAYGFEQATEYHKQSPEIE"
     gene            12182..>12782
                     /locus_tag="VF531_15920"
     CDS             12182..>12782
                     /locus_tag="VF531_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX7715506.1"
                     /translation="MPLSDYEIVIGLEVHAELKTESKVFCGCTTKFGGEPNSQVCPVC
                     LGLPGVLPVLNQKAVECTIIAGLALNCEISHYSKFDRKQYFYPDLPKAYQISQYDDFP
                     LCKKGWVEIPDSQNPGRMKRVGITRVHLEEEAGKSVHSGAGILNSDYSMEDYNRCGIP
                     LIEIVGEPDLRSPEEAKNYMERIRTLLLYTGVSDCKMEEG"
BASE COUNT         3106 a         3014 c         3452 g         3210 t
ORIGIN      
        1 atgacacttg gcccgggacc cggcagtttc ctggagaaat tcaatcccct taccggacag
       61 gtaatttgga gacagaacat cgcgatcagc gggggaatca actgcggacg atacaatcgg
      121 gccgaccgtt gcacttatct ggtggatggc aacggggtgc ttaagtttga tccccaggga
      181 cggcagctgg gcagcatact ggatttaaaa cagcagagta tcctgggatt tgagactgtc
      241 gttaccggta tcaatgtggc gaaaggcgcc cagtatccgc tgatctatct catcaacttg
      301 aaccagcaag tccggaacct gtataattcc ggccaaccgg tagccgcaaa cggaaaacca
      361 tataatattt atcaatattc atatgtgccc aagctaccca aagagagaaa tcaaaaagtc
      421 atcacgattt ccacgctgat gccggatcgg tttttagaga ccgcgatcag cgcttatcag
      481 agcaaacacc cggggatcaa gattgaattg aagagttttg taaacggcaa catcatcacc
      541 aaggtcgaga tggaacaatt cgttaaatcg gtaagtgccg aattgatggc gggtaaggga
      601 cccgatatcc tttccttggg gtcctcacct tttcagaaat tggcggatcg taacgtcttg
      661 gttgatctgg gcgctttgat ggccaaggat aaaagttttg accggaacaa atattattcc
      721 aacatcctgg atgcctgtaa atataaagac ggtctctccg tattgccggt attcattcag
      781 tttgacgccc tggagctgaa tccggaaatc ctcgataaag ccggggttcg catcgatgat
      841 acccggtgga attgggatga atttttaaaa gttgccgaaa aagtgagcct ggatatcaat
      901 ggtgacggtc gcccggaaca gtttgcactg cccaatatgg atcgactccg tttattcaac
      961 tggatgttcg aaacccagta ttcatccttt attgaccggg agaaaaagaa agcctatttt
     1021 gatacgccgg aatttatcgg gctgctgaat ctttgcaaga ccttcagtga taagcagttg
     1081 atgagtacgc aagccgatcc caacgcttcg caatttaaga ttattattaa tggacaactt
     1141 atggataagg gggctatcag caccgtagcc tttaccccca ccaccgtttt tggttatagc
     1201 agccttctcg gggccaagac aacctttcat accgcccgct tacttagtct tcccggaaac
     1261 ggcgcgggca gcggctttga gacaccgctc ggcatgcttt ttggaataaa ccgtaagtct
     1321 aagctgcaaa aggaagcctg ggaatttcta aaatatctgc tttcggatga agctcaaacc
     1381 ttcccggggg cgatggggtt tgcggttaac aaggatgccc agagaaaaag ggcggccgca
     1441 gagattgatc gggagacggc ccaggagatt gccaaccccc tgaatgaatc ggatatggag
     1501 caagtggacc gactcatcac caatctgcgc cagttgaata ataattttga tccccagatg
     1561 atagccatca tcgatgatga gttaaggccc tttttcagcg ggcaaaagtc cgcctcggaa
     1621 gtggcgaaac taatccaaag caaggcaacc acctatttgc aggagtaagt taatcccagg
     1681 gttattgatt tttttagcgg atcagtccaa acaggaactc ataaaaaaac gggaatttat
     1741 cattgaataa aaataatggc ccggtcttag aattataaga cgggcctttt ccattcgatt
     1801 cgaaaggaga attaatatcc aaccatcctt atcaggttgg gccggttttg atataataga
     1861 tggcagcctg gtttaaggga atagttgaaa ccccgggatg cgaaccttat ttggtttcac
     1921 gtttgatggc tgccagacag tcggcgcaga ccattttgtc tttgaagttt ttgacaccgt
     1981 tcatttcacc gcagaagatg cagcccgggg tgtatttttt caggatgatt gaaggctgct
     2041 cctcatcgat gaagatctcc agcgggtctt tgactgcgat ataaagttgt tcccttgtct
     2101 ccttgggaat aacgatgcgg cctagttcat ccacttttcg cacgataccg gttgttctca
     2161 taaaatgtga cctccatacc gacgattttt cacgcttacg cgtgacgcgt catacgtgat
     2221 tttaataaac attatacaat tcgctccatg atcagtaatt ccttccggga tctcaggaaa
     2281 atgccggcaa tccgcttaac tttttattac aacggaaata ccgggatttc ccaggttgga
     2341 atacgggttt tcgcagggag tgaacgatga aaggatggct ccaaattcat gttacagttt
     2401 tccgctgcga tccgggaagg gatcgaccaa atctttagaa atgctgccgc ggatgggcgg
     2461 aggatgctat acgaacatga ggtatacggg gtactgagac tgcttgattt ggcggtgccg
     2521 gagtttatcc tgatcgaaca tgttgcagaa cttaccgagg agcagctcaa gccgttcggc
     2581 cgggaagttg tggtaaagat tgtttcaccg tggatcgccc ataaacaaaa agttggcgga
     2641 gtgaaagtgg tctctaatac cggggcgttg ttcatccagt ttgtcatgac ccggatgaag
     2701 gaagaggtgc tggggcattt tcccgctacc gatccaccgg tgatcaaggg atttttgatc
     2761 gaggagtaca tcgatttctc ccaggccctt ggttatgaaa cactgatcgg gattaaggaa
     2821 gatccggctt tcgggccggt cctgaccctc agtaaaggag gtgatgatgc ggaattcttc
     2881 gcccgttatt acgatccggc aaactccttt ctgcctccgc tcgattccgt cacagccctg
     2941 cggatgcttc aaaccctcca tatcaggcat aaatttgaag ccatcggcca tcccgagtat
     3001 ctggattata tggcccgggc tgtagtcaac ctgagtgccc tggccacggc ttactccagg
     3061 gtggatactg cgacaccgca atatatcatt gagacattcg aagtcaatcc tttcgtcatc
     3121 acccgggatc atcgtctggt ggctttggac ggctatgctc agttcgaacc ggcatcgggg
     3181 gttgatgata gggtgaccgg actggctctg accaacctgg acagcttttt tacaccccgg
     3241 ggtatcgcgg tagttggcgt ctccgccgat ccgagcaagt atagtttagg ccgggagatc
     3301 gcaatgttgc tgcacgatct gaaccggacc gacctctttc tggttaatcc gaaagggggt
     3361 tcattggaac tgaacggaac gactttcccg ctgtatgaaa gtccggcatt gctgcctcag
     3421 ccggttgacc tcatggtata tacggctccg gcccaatata tgctggattt catcaggcaa
     3481 ttggcggacg agccggatac tcgccggccg cgagggttga tcatgatacc cggtattccg
     3541 gcggatcttg attacgccgc tttcgcccaa cgtttagcaa cacttgttcc ggccgggtta
     3601 cgcatcatcg gccccaactg catgggagtt tatttcgccc cggatcaatc aaatcccggg
     3661 gtaaacaccc tctttatccg ggaacagcgg ctggagctaa aatcctctca gtttagcaat
     3721 actgtgctgc tcacccagag tggagccttc tcggtgactg ccattgataa actccgtaac
     3781 gcccgcgttt ttcaggcgat tgtcagtttc ggcaataagt tcgatgtgaa gatcaccgat
     3841 ctgatgtctt atttcgcggc ttccccgcgg atagacgtta tcgctctgta catcgaaggg
     3901 ctggaccgtg gtgaaggccg ccgtttcttt gaattggcgg ggacgattcc caaaccgatc
     3961 attgtttata aagccggtcg aactctagcc ggtgccaggg ctgcagcttc ccacaccgcc
     4021 tcaatgaccg ggagttacga agtttttggt gccgcctgcc gccaggcggg ggtcattctt
     4081 gcagaaaacc tggaggatca ttacaactat atccggacat ttagcctgct gtcacggagg
     4141 cctccgcggg gcaaccgggt tgccggcgta gtcaatgccg gttttgaatc gacagtgggt
     4201 gccgatgaac tgaaaaattt ggcccaggca aaactgggtc cggagacggt ggtgcgattg
     4261 aaccggatca accggttcgg gctggtggac accacggcgc cgtttttgga tatcacccct
     4321 atggccgacg accggatgta tgccgagttc gtcgaggcca tcctggaaga tgccaatgtg
     4381 gactgcgtgt ttgtggccat tgtaccccat accccctctc taaagaccgg accggaggtt
     4441 tgccatgatc cggatggatt ggcttggcaa cttgtagagc tgtaccggaa gtatcccaag
     4501 cctttggtgg tttcggtgaa tgccggccgt tattatcaag agttcgtctc ggtcatggag
     4561 gagaacggga tcccggtctt taccgatatc cggtctacca ttaactccct ggaccaattt
     4621 gtccggtatc atgtaaaagg gaaaaacctg aatcttgggg aggagaaata aaaatcacgg
     4681 cgaatagaag cgattaatat ttccattttt ggtagggtgg tttcttcttg cggcgggcgg
     4741 ctctgtcgcg ggaagtggta ggaagttcat aggacggtag gaaaaaagga aggatggtac
     4801 aaatgaatat gttacttcta ttccgttggc tggcggtagc tgcttttgcc tgcctgatgg
     4861 tggggatcgg cctttatgcg ttacgccgga ccaaaaccag ctccgacttc ttcctggctg
     4921 gaagaaatgt gggaccctgg gtgtcggcgt ttgcttacgg caccacctat ttttcggcgg
     4981 tactttttat cggttatgcg gggagtcaag gttgggggtt tggcattagc gttctttggg
     5041 ttgcccttgg aaacgccctc atcggcagct ggctggcatg gaaggtgctg gcgcggcgga
     5101 cccggtcaat caccgcccgt ctcggggcga tgacgatgcc ggagttcttg gaaacccgtt
     5161 atcagtccaa gggattaaag attgcggccg ccatcttaat cttcgttttt ctcatcccct
     5221 attgcggttc ggtcttcacc ggactcggct ttctcttcga aaacgtcctt aacatcaatt
     5281 atcagctatc attggtggta atgatcgttc ttaccggaat ctatctgatt ttgggaggat
     5341 atttcgccat tgccttcaat gatttcatcc agggattaat catgctggtc ggagcggtgt
     5401 tgatggtagg atatgtattt catcaaccgc aagtgggtgg ttttggggga tttattccag
     5461 gactcgcccg gatcgacccc ggattgacgc aagtcattcc gaccaattgg ctcggcctgt
     5521 taagcctggt gatattgacc agtttgggaa catggggcat gccccagatg gttcagaagt
     5581 tttatgccat taaaaatgag caggtcattc ggacggccac catcgtggcg accctgttcg
     5641 cactggttat cgccggcgcc gcatatctgg tcggctccac agtacacctc ttttttaacg
     5701 ctgtgccggt ggacgctgcc ggaaaccccg tctacaacac actgattcca cagttgctaa
     5761 atcaggtctt acccgaggcg cttctcatca tcatattgct gctggtatta tcggcttcca
     5821 tgtccactct ctcctcactg gtgctggtct ccagctccgc gctgaccatc gatctgatcc
     5881 agggagtctt tcatccgggg atcgataaaa agcattccct gacattactc cggctcttct
     5941 gcggactctt cattctctgc tccattggtg tggcggtctt taaaatcgac ttcatcattc
     6001 aactgatggc cttatcttgg ggcacgattg ccggagcctt tttggcaccg tatctgtacg
     6061 ggttattttg gaaaggtacc accaagagcg gtgcctgggc cggtctcatc tccggcatcg
     6121 tgatctccat cggtggttcg ctggtatttc cggacaaaaa tacggctccg ttaatcagcg
     6181 ccgtggcgat gctggttccc ttgggggtag tgccgctggt gagcgtattt acaaagaaat
     6241 acgacgccgc gcatttggag cgggtttttg gtccggccga acccccattg acgctggcag
     6301 ccagcacccg ggagtaattt aatttgcctt aaagcgttgt atatcgatct caaggcttaa
     6361 ggaattagct caagtctccc ccgggtttga cgtcatcaga tcgtggaata acgactgccc
     6421 ccaaagggca gtttttttca tttcatcatt ttttttgtaa taaaaagtga ggtggggacc
     6481 tagactaata ttaagtatcg ctttaaataa atatgcgccc cgacaattca aaaagcaaca
     6541 cggtatttta aaccaatttg gatcttctct gattgtaaat tggcaacaat gatcttcgaa
     6601 aaaaatttcc gaagtattac attttgtggt agaaaaaaat accctcattg acgatattat
     6661 atataagaga aagttcagaa cactcctggt cggatggatg gaaatcccgt catatgccaa
     6721 tatgaatacg tttggaaata atgtatcttg ggtatggcgt tcgccggata aaaatagaat
     6781 tcggattgta aacataggac caataaaccg cgtttgcaag tgggatcccc gctcacttca
     6841 aataaaatca aaatgaaacg gaggtgaaat ggatgcgatt actaccggtt gggttacaaa
     6901 ttcggccgat ggcggaagaa gattaccgtg atgtgtatcg tttataccgg caattggttg
     6961 ggactggcct gacgtaccgg gctaccgcta ctcaggagca acaggaattt tcacggattt
     7021 ttcaaaactt ctttcaaaac ccggaacggg aagcctatgt cgcggcggtg tatgacaatg
     7081 tgattggttt cgtgaccctg tattacctgg atgtgtttca tcattccgga cagatcgcca
     7141 atattcagga attagtggtc acggaagaat tccggggtcg cggtgtcggc aaagccttgg
     7201 tcgattttgt aaaaaataaa gcgctagaaa aacactgtcg gggtttggaa gttgcgacgg
     7261 atctatggca gagcggggcg aaatcgttct ataacaaatg cggtttacat ggtaaggttt
     7321 tgatgatggc tacctgaggg ttaagaaaac aaggaaactt aaacttaagg tgaaccttga
     7381 caaactagct ctactccggg ctagttttct ctttgcagca agtattttta caagatttgg
     7441 ggcaggattt taagcgggag tgtataatac taagttactt tcaactgaat attcaaataa
     7501 aaagaactta atatttcaga ccaatttgca gcttatttat ttattagtcg gttacaaatt
     7561 ggtataatac caataagcaa aatccaaaag aatcctattt gaaagcgctt tccatcttat
     7621 aaaaacggag gccggcggat gactcactcg gatttagtac cggatgatat ccgaacaaaa
     7681 gttgcaaggc ttcgccaaac catcgaacac catagctatc aatattatgt gctggacagc
     7741 ccggaaattg acgatcgaca atacgatgcc ttaatccatg aacttgcggc cttggaggcg
     7801 gagtatccgg agcttcggac accggattcc ccgactgtac gggtgggcgg taaaccgttg
     7861 gaactttttg aaacagtccg gcatcaagtg ccgttgttga gtctggataa cgcctatagc
     7921 gccgaggagt tgactgagtt tgacaaacgg gtcaagcggg cgctgggtag cgacgccgag
     7981 ctcgaatatg tagcggagct caagatcgat ggtttgactg ttgccctgac gtatgagcgg
     8041 ggattattga ttcgtggcgc cacccggggg aacggcgagg aaggcgagga tgttaccatc
     8101 aatgtcagga ccatcaaatc aattccgcta cgacttccat ccagcctctc cggagtatcc
     8161 ctgctcggtg tcaggggaga gatttacctc aaaaaggctg attttcttgc cttgaacgaa
     8221 caacgcgaga tccgtgagga gccgctcttt gccaaccccc ggaatgcggc tgccggatct
     8281 ttgcggcagc tcgatccgaa gatcactgcc ggaagggctt tggatgcctt tttttatgac
     8341 attcttttcc ttgaaggccg tcaagtgccc agtcagtggg atgggttcga attgatgcgc
     8401 gccgcacgat taaagaccaa tccgaattct aagcgttgcc aggggattgg ccaggtgatc
     8461 gagtactgta actactggac cgaacaccgg ggagaacttc catacgaaat tgacgggatc
     8521 gtggtaaagg taaacgactt gcgactccaa ggccagctcg gcttcaccgc caaggccccc
     8581 cgctccaaga ccgcctttaa attcccggcg gaacaggtgg agaccaaggt ccttgatatt
     8641 caagtgaatg tgggacgaac cggagcagta actccggtgg cgatcctgga atcggtgagg
     8701 gtggccggtt ctacggtgag ccgcgccacg ttgcataatg aagataatgt taaggaaaag
     8761 gatattcgcg tcggtgaccg ggtgatcatc caaaaggccg gagatgtcat tccggaggta
     8821 gttcgctcac ttcccgagaa acggaacggc agtgaaagga tttttaccat gcccgtggtc
     8881 tgcccggaat gcggctctga ggtttaccgc gagccggggg aggccgtggc gcgttgcatc
     8941 ggagcgactt gtccggccca actccgccag ggtatcatcc atttcgtatc ccgcgaagcc
     9001 atggatatcg aggggctggg cgaagcaatc gttattcagt tgatcgattc cggattggtc
     9061 aaggatgttg ccgatctcta tgaattacgc tatcaagact tgattgagca ggagcgtttc
     9121 ggcgccaaat ccgcagccaa cctgctccag gcgattgagg aatccaagaa gaacgatttg
     9181 agtcagctcc tgtttggatt gggaattcgt cacgtgggcg cgggagcagc ccgggaactg
     9241 gcgtcgcgtt acgggtccct tgaccggttg atgcaggcaa gttatgatga gctaaccgcc
     9301 atcgccacta tcgggcctaa gatcgcgggc agtgtaatta agtatttcga ggaacctcat
     9361 aaccgggaag ttatcgtcaa gttacagcgg ttggaggtag accccagaga atccggcgcg
     9421 ggtgatacgc cgcaaaccct ggaggggaag accttggtgg tcaccggaac cctggaatct
     9481 ttcggccgta cggaggtcga agagatcatt cggcgccacg gcgggaaatc cgcatcaagt
     9541 gtaagcaaac gcaccgacta tgttctggcc ggggccgatc ccggctccaa acttgagaaa
     9601 gcccgaagcc tgggggttag ggtcattgac gaagcggagt ttaaggcatt gcttgagggt
     9661 gggtcatcca accgctagct tcaatataaa atcaacaacg agacatacga aactcacgaa
     9721 aatgttgact tgaaaattta tttgctaaaa tgtttaactt catttattta tgatccattt
     9781 tcgtgttttt tgtgtatttc gtggttagga aattagtata cccctttcaa agttataagg
     9841 ttaactgaaa gaatacgagt ggtccgaagg gatgcggcac cggcagcttc gttatagagc
     9901 taaagtaacg ctttgatttt gaacccaatt ggcaaaaaat tgtatgatgg cgcttgatta
     9961 tatgaatctt ggaggttttc aaaatgggtt cggagatgca tgatcaaaaa aagacgactc
    10021 tttccagtga agatgttcgg catgtggcga ggttagcccg gttggaattg aactccgccg
    10081 aggaagagca attcacgaag gaactcaatg atattctgag gtttgtcgag cagttgaacg
    10141 agcttgatac cgcggggatc gaaccgacgg cgcacgccat cacggtccgg aatgtattcc
    10201 ggcctgaccg tgccggggtt tccctggatc ccgaggcggc tctggccaac gccccggacc
    10261 ggagcggtaa tttctttaag gtccccaaga tcctggagga ctgaaccgga attcgggttg
    10321 tgcagaatgt ttgggagggt cgtcatttgg aattatatca aaaaactgct catgagcttc
    10381 atgatttaat caggcgcaaa gaagtccgtg ccgttgaagt ggtcgcctcg gtctttgatc
    10441 gaattcgggc ggtggaatcc aggattaact cctatattac cttgaccgag gaacaggctg
    10501 tccatacggc aaaacaggtc gacatgatgg tggagaaggg agaactgttg ccgaagctcg
    10561 ccgggattcc ggtggcgctt aaggataatc tctccacccg ggggatccct acgagttgcg
    10621 gttcgaagat gctgacggat tacataccgc cttacgatgc caaagtagtg gagctgctcg
    10681 cagccgggaa gatgcctctg gtcggcaaga ccaatctgga tgagtttgca atgggttcat
    10741 ccaccgagaa ttcggccttt catattaccc gaaatccctg ggatccggat tcggttcccg
    10801 gtggttccag tggcgggtcg gctgcggcgc ttagcgccgg agaaaccatt ttggccttgg
    10861 gctctgatac cgggggttca atccggcaac ccgcaggata ttgcgggatc gtcggtatga
    10921 agcccacgta cggcagggtc tcccgttacg gcttggtagc cttcgcttca tcgctggatc
    10981 agatagggcc catggccaag gacgtaaccg attgtgcgct tttgctccag gccattgccg
    11041 agtatgaccc gagagactcg acctgtgtca accatccggt acccgactac cttcgggaga
    11101 tgcagcgggg agtcaagggc atgcggatcg gtctggccca agagtacctg ggtgaaggga
    11161 tcagcacttc ggtacggtcg gcgatggatg aagcggtgaa agtcttccgg gatttgggag
    11221 cagagatcgt cccgataacg ttgccccata cccggtacgc cctggcgacc tattatgtgt
    11281 tggcaccggc ggaagccagc tccaatctgg ccaggtacga cgggttgcgt tacggacacc
    11341 gtacctcggc tcaagtttcc gactcggtgg agatgttcat gaaatcccgt tccgagggtt
    11401 tcgggcctga ggtcaaacgg cggatcatga ttggcactta tgttttgagc gccgggtatt
    11461 atgacgccta ttacaagaaa gcacaacagg tccggacttt acttagggat gattataata
    11521 aggcttttag cggctgcgat ctgatcttga ctccgaccac cccttcggtg gcgttcaaga
    11581 ttggctccaa gactcaggat ccgctgacga tgtatctcga ggatatctgc accatcacct
    11641 gcaatctggt ggggataccg ggtatctcga taccttgtgg ttttgatcac ggtctgccca
    11701 tcggcatgca gttgttgggg ccggctttcg gagaaggctc gttactccgg gcggcttacg
    11761 gatttgagca ggcaaccgag tatcataagc aaagcccgga aattgaatag gcaaaaggca
    11821 acaggcaaca ggtatgagag gcagaaagct tgcaaaactg gttttgaaag ggtgcaggga
    11881 agccctgccg gggactagcg tccggaacgg acgctagtcc ccggtaatca atccaaactg
    11941 cccctcctcg aggagggggc gggggaggtt ggcccaatat ttctacaaaa gtggtagaaa
    12001 gattaaccta aacttgcctt attgagatag gtaacaggca aaaagagtgc ttaagttgat
    12061 gtttccaagt tctaagttaa aaatcttttt tctactttat tcgcttgact tttcactact
    12121 tactgttgcc tgttgcctgt tgcctatcca ctattgccta cgtcattgaa ggaggattta
    12181 catgccctta tccgattatg agatcgtgat tggtctggaa gttcacgctg agcttaagac
    12241 cgaatccaaa gtattttgcg gctgcaccac caaattcgga ggcgagccca acagccaggt
    12301 gtgcccggtc tgcttaggac ttcccggagt tttgccggta ctgaatcaaa aggcggtgga
    12361 gtgtacgatc atcgccggat tggcattaaa ttgcgagatc tcacattaca gcaaattcga
    12421 ccggaaacag tatttctacc ctgatctgcc caaggcctat cagatctcgc aatatgacga
    12481 ttttccacta tgcaagaagg gttgggtgga gatcccagac agccaaaatc caggccggat
    12541 gaaacgggtc gggatcaccc gggtccacct ggaggaggag gccgggaagt ctgtccattc
    12601 cggagcaggg atcctaaact ccgattattc catggaggat tacaaccgtt gcggcattcc
    12661 cctgattgag atcgtcggcg agccggatct gcgttcgcct gaggaagcca agaactatat
    12721 ggagcggatc cggaccttgc tcctttacac cggagtatcc gattgcaaaa tggaggaagg
    12781 tt
//
LOCUS       DASVMB010000569         6404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_146015,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000569 DASVMB010000000
VERSION     DASVMB010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146015"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF531_15925"
     CDS             <1..269
                     /locus_tag="VF531_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7715507.1"
                     /translation="APVNELFAEPLHPYTQGLLNSIPRLSEKRERLYVIDGMVPDLSA
                     VPRGCAFHPRCPQAGPRCRRERPELTDEPGGRQIACWLREGGVA"
     gene            269..1234
                     /locus_tag="VF531_15930"
     CDS             269..1234
                     /locus_tag="VF531_15930"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX7715508.1"
                     /translation="MAELVTVSHLKKYFPVDRTIFGRVLANVKAVDDVSFTICEGETL
                     GLVGESGCGKSTTGRLLLRLLEPTSGQITFDGHELTSYSAEEMRKLRREMQIIFQDPY
                     ASLNPRMTVGRIVAEPLIRHGIARGAKCRDRVAELLTCVGLDPNYSRRYPHEFSGGQR
                     QRIGIARALATNPRLIICDEPVSALDVSIQSQVLNLLKDLQNEFKLTYLFIAHGLNVV
                     KHVSDRIGVMYLGKMVELAGGDELYAHPLHPYTEALLSAIPVPDPTAKRERIILSGDV
                     PSPINPPEGCRFHTRCPKAMEVCRVKEPELIENKGHLVACHLVNK"
     gene            1462..2793
                     /locus_tag="VF531_15935"
     CDS             1462..2793
                     /locus_tag="VF531_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX7715509.1"
                     /translation="MKLSPSQVLALGFAGLILLGALLLTMPMASKDGQSLSFLNALFT
                     ATSAVCVTGLVVVDTGSHFTPFGQFVIIFLIQVGGLGFMTFSTLAAILIGKKIGLKER
                     MVIQESFNQDTLAGLVKLTRNVLWVTLIIEGAGGLILTLRFLADFPLGRALAFGFFHA
                     VSSFCNAGFDLFGQVFGSYSSITHYVDDWIVTLTVGGLIILGGIGFPVIMELVRFPRK
                     KRLSLHSKLVIVMTVVLIGVGALLIFLIEFANPKTIGGLSVSGKFLGALFQSITPRTA
                     GYNSLDISKLRTGTWFIMIILMFIGASPSSTGGGIKTTTFGVLVATIITSIKGRQDAE
                     LFERHIPTDIIYKAITILTIALSWVSVATLVMSLVEPYAFIRLFFEVMSAFGTVGLTT
                     GITPDLTVISKIIIIITMFIGRVGPVTILIALSRTEKHNSVKFVEERLMIG"
     gene            2858..3514
                     /locus_tag="VF531_15940"
     CDS             2858..3514
                     /locus_tag="VF531_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX7715510.1"
                     /translation="MAKQYVVIGMGRFGTSVAKTLGKLGQEVLAIDTDEEKLHYVADD
                     VTHTLVADTTDAHVLESIGMKNFDVAVVAIGENIQANIMTTLLLKEIGVPYVVAKAVN
                     KMEGRVLEKVGADRIVFPERDMGKRIAHNLVASNLIDYIELPEGFRIMEVIATKEMFG
                     QNLKTLNLRAKYNVNILLIKKPSGKLLFTPGPGDVVEIGDLLVVAGEKEAIDQFEQNI
                     "
     gene            3949..4353
                     /locus_tag="VF531_15945"
     CDS             3949..4353
                     /locus_tag="VF531_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX7715511.1"
                     /translation="MDKEPLEKHIFELEDRLLQPEVRSSAEKIAELLHIDFFEYCSSG
                     SVYCYRKGDVFDEKNGSATVHREIKDFEINLLSPDVVLATYKVIQHDELSQNLKYSLR
                     SSIWKCFNGEWQIVFHQGTLTDSFHEKPEGIP"
     gene            4399..4875
                     /locus_tag="VF531_15950"
     CDS             4399..4875
                     /locus_tag="VF531_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715512.1"
                     /translation="MKRKRSNYNTLTEKYLPSEPCRCEICLAYCRRPGWWTVAEAAQA
                     MDAGYAHRMMLEISPGFTFGVLSPAFKGCEGGVAVNIHAHRGCNFLVNNSCELYNTGF
                     MPLECRFCHHDRVGRGQQCHADIEKDWNTADGQRLVIKWGNRVRLWEHNYSFYLKG"
     gene            4937..5656
                     /locus_tag="VF531_15955"
     CDS             4937..5656
                     /locus_tag="VF531_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7715513.1"
                     /translation="MNRFLKVTSLIIAIAIFIIVANYVIVVIRAREMTPKIISGALKA
                     EDNPLKIGDLSDWQIRALLKIEDPAFYQHKGVDLRTPGAGLTTITQSLVKIYYFDHFK
                     PGFAKIKQTLIARFALDPLVSKNDQLQLFINHIYLGTVNNRPVVGFVNAARVYYQKEV
                     NQLTERDYLSIVAMINSSRAFSISDRPGANRERTERLILVVEGKYIPTHLMDMYYGKL
                     DSATQKLLAPSSYFPGIYEKK"
     gene            5695..>6404
                     /locus_tag="VF531_15960"
     CDS             5695..>6404
                     /locus_tag="VF531_15960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7715514.1"
                     /translation="MKPNKDLVLLYLFNFFKSLQFFGAVAVPFYLHRVGLDYTRMFFL
                     ETVFSFGMIVLEIPTGVIADRWGRKLSLVLGSCFFGAGFLLFGIYTSYFILVLAEIIC
                     ALGMTLLSGADRAILYEVLKRESEEARAGAVMARYDSLGTAAMFLAFPAGSLFVGSKL
                     VPYKTALGLVFVATAVAIAISGLIILFVKEGVYEKSRANPFRQGFEGFRYIFDHPKLR
                     VFSLNYALISSMTFFMFW"
BASE COUNT         1619 a         1352 c         1614 g         1819 t
ORIGIN      
        1 ccgctcccgt aaatgagctc ttcgccgagc cgctacatcc ctatacccag ggactattga
       61 actccattcc ccggctctcg gaaaaacggg aacggttata cgtcatcgac ggaatggtgc
      121 cggatcttag cgccgtaccc cgcggttgcg ccttccatcc ccgctgtccg caagcgggac
      181 cccgttgccg cagggaacgt cccgaactta cggatgagcc gggcggccgt caaatcgctt
      241 gctggcttag ggaagggggt gtggcctgat ggcagaactt gtaacggttt ctcatttgaa
      301 aaaatatttt cccgtggatc ggaccatttt cggccgggta cttgcaaatg tcaaagctgt
      361 ggatgatgtc agctttacca tatgtgaagg agagacctta ggcctcgtgg gtgagagcgg
      421 ttgtggaaag tccactaccg gccgcttgct gctacggttg ttggaaccga cttcggggca
      481 gatcactttc gacggccatg aattgacatc ctactcggca gaagaaatgc gtaagctccg
      541 ccgcgagatg caaattattt ttcaggatcc ttacgcctcc ttgaacccac ggatgacggt
      601 gggccggata gtcgccgaac cattaatacg tcacggtatt gcccgtggcg ccaaatgccg
      661 cgaccgggtg gccgagcttt tgacctgtgt cgggctggac ccgaactata gccgccgtta
      721 tcctcatgag ttcagcggcg ggcaacggca acggatcgga attgccaggg ccctcgcaac
      781 caatccgagg ttaatcatct gtgatgagcc ggtatcggcc ctggacgtat ccattcagtc
      841 ccaagtgctt aacctgttga aggatttaca gaatgagttc aagctgacat atctcttcat
      901 cgcccatggg ctgaatgtcg tgaaacatgt gagtgaccgg atcggtgtaa tgtacctggg
      961 aaaaatggtg gagttggccg gcggagacga gctttatgcc catccgcttc atccttatac
     1021 cgaagcgttg ctttcggcga tccctgtacc tgatcccacc gccaaacggg aacgcatcat
     1081 cttgagcggc gatgtgccca gccctatcaa tcccccggaa gggtgcaggt ttcatacccg
     1141 ttgccccaag gcgatggagg tatgccgggt taaggagccc gagttaatag agaataaagg
     1201 gcatttggtt gcctgtcatt tggtgaacaa gtaagatagt ttttagagta tttatatgag
     1261 taacatcttt gcacccgtac agcgaaacgc tgtacgggtt tgtttttaga agcatcggct
     1321 cgaaagggac actcattgac atatctattt tatggtatta atattatcac attgacttat
     1381 gcccaatgaa gacgaataaa caatttacat atttcatatt aaaccaattg gcggagttga
     1441 aaaaggttgc atctatccaa tatgaaacta tctccatccc aagttcttgc cttaggtttt
     1501 gccgggctaa ttctattggg agcgttgctt ttgaccatgc ccatggcttc aaaagacggc
     1561 cagtcgctaa gttttctcaa tgccttattt actgccactt ccgcggtatg tgtaaccggt
     1621 ctggtggtgg tggataccgg gtcacatttt acgccattcg gccagtttgt gatcattttt
     1681 ttgattcaag ttggtggatt gggctttatg actttttcca ctttagccgc catcttaatc
     1741 ggcaaaaaga ttggtcttaa ggaacgaatg gtgatccagg aatcatttaa tcaagatacc
     1801 ctcgccggtt tagtgaaatt gacccgtaat gttctttggg ttacccttat aattgaagga
     1861 gcgggagggt taattctaac tttgcggttt ttggctgatt ttccactggg aagggcgctg
     1921 gcttttggct ttttccatgc ggtttcgtcc ttttgtaatg cgggttttga tctgttcggc
     1981 caagtttttg gaagttattc cagtatcact cattacgtcg atgactggat tgtcacgtta
     2041 accgttgggg gattgattat ccttggagga atcggttttc cggtcattat ggaattggtt
     2101 cgctttcccc gtaaaaagcg gctttcgctt catagtaagt tagttatcgt catgaccgta
     2161 gttttaattg gtgttggggc attattgatt tttttgattg aatttgccaa tcccaaaaca
     2221 attggaggac tgagtgtatc cggtaagttt ttaggagctt tattccaatc gattacaccc
     2281 cgtactgccg gatataactc gctggatatc tcgaaattac gaaccggtac ctggtttatt
     2341 atgattatcc tgatgtttat cggcgcgtcg ccgagttcta ccggaggcgg aatcaaaaca
     2401 accactttcg gagtactcgt cgcaacaatt atcacaagta tcaaaggacg gcaagatgca
     2461 gaactgtttg aacggcacat tccaacggat attatatata aagcgattac tatcctgaca
     2521 attgctttaa gttgggtatc tgtggcaacg ttggtgatgt ctttggtcga gccctatgct
     2581 ttcatccggc tgttttttga agtaatgtcg gcttttggca ctgtaggatt aactactggt
     2641 attacacctg atttgacggt tatttccaag attatcatta ttattacgat gtttatcggt
     2701 agggttggac cggtaaccat ccttattgcc ctaagcagaa cagaaaaaca taactccgtt
     2761 aaatttgttg aagaacgctt aatgattgga taattctttc gcccatgata tgcgcgcgta
     2821 atacgaaaga gaaaagtatc ctcaaggggg attatgaatg gctaagcagt atgtggtaat
     2881 tggcatggga cgctttggaa ccagtgttgc caaaaccctg ggtaaactgg gacaggaagt
     2941 attggcgata gacacggatg aggaaaaact ccattatgtt gctgatgatg taacccatac
     3001 tttggtggcg gatactacgg atgcccatgt gctcgagagt attggtatga aaaatttcga
     3061 tgttgccgta gtggccattg gagaaaatat tcaggccaat atcatgacca ctcttttgct
     3121 aaaagaaatc ggagtcccgt acgtggtagc caaagcggtg aataaaatgg agggaagagt
     3181 cttagaaaag gtaggcgcgg atcgcatcgt ctttcccgag cgggatatgg gtaaacggat
     3241 cgcccataat ctggtggcct ccaacttaat cgactatatc gagctaccgg aaggatttcg
     3301 gattatggaa gtcatcgcca cgaaggaaat gttcggccaa aatttgaaga cccttaactt
     3361 acgggcgaag tataatgtca atattctttt gatcaaaaaa ccttccggta agctcttgtt
     3421 tacacccgga ccgggagatg tcgtggaaat cggcgatctg ttagttgtgg caggtgaaaa
     3481 agaagccatc gaccaatttg aacaaaatat ctagttggag acatttatcc tttttgaaac
     3541 cgtactgcga attgcagtgc ggtttttttg gttttccggc tggaatgggg ggtgaaacct
     3601 ccagaattgt ttaccaaaaa ataattttct gcccggaggt gaggggaaaa ggttgcggaa
     3661 agtattatat ttgagttact gattataaag atcagtctgc aattcaccaa gccgtatgtt
     3721 gaatgcctaa cgacaactca cccctcgaaa gatattgctg atacagtatt tgtctggccc
     3781 ctctctactt tgatgataat ttttggtgtt gatcagtaga gaggggctgg ggggtgagtt
     3841 tgttattaga caaattggac tagctaacat tctgtttgct gaggtaactt tataaccagt
     3901 tttaagttta cgaagttcat gaatcccggg acaggttggt gaaagccgat ggataaagaa
     3961 ccgcttgaga aacatatatt tgaattggaa gaccggttgt tgcagcccga agttcgcagc
     4021 tccgcggaga agatagccga gctactgcat atcgacttct ttgagtattg cagttcgggg
     4081 agtgtctatt gttataggaa aggcgatgtc ttcgatgaaa agaatggttc tgcaacagtc
     4141 catcgggaaa tcaaggattt tgaaatcaat ctgctctccc cggatgtagt tctggctaca
     4201 tataaggtga ttcaacatga tgaattatcc caaaacttga agtattcact gcgcagttca
     4261 atctggaaat gttttaacgg agagtggcaa atcgtttttc accaaggaac gttaaccgat
     4321 agctttcatg aaaaacctga ggggatacca tgagatattc aggctataaa ttcggcaccg
     4381 cgttgggggt gatccgaagt gaagcggaaa aggagcaatt acaatacact caccgagaaa
     4441 taccttcctt cagaaccttg ccgttgtgag atatgtttgg catactgccg gcgtccgggg
     4501 tggtggactg tggcagaggc ggcccaagcg atggatgccg gatatgccca ccggatgatg
     4561 ctggaaattt ccccggggtt cacttttggt gtgctttctc cggcgtttaa agggtgtgaa
     4621 ggcggagtag ccgtaaatat tcatgcgcat cggggatgca atttccttgt caataactca
     4681 tgtgaattat ataatacggg ctttatgccc cttgaatgcc ggttttgtca tcacgatcgc
     4741 gttggtcggg ggcagcagtg tcatgcggat attgaaaaag attggaatac ggcggatggc
     4801 caaaggttgg tcataaaatg ggggaatcgg gtccggctat gggagcacaa ttatagcttt
     4861 tacttgaaag gttaagtgtt acagtcggcc gtttcagcgt tataacctct gttttttgtg
     4921 gaaggagaac gattcaatga accggttcct caaagtgaca tctttgataa tcgccatagc
     4981 catttttatc attgttgcca actatgtgat cgtcgttatc agggcccggg agatgactcc
     5041 taaaatcatt tccggggctt taaaggcaga ggataaccct ttaaaaatcg gcgatttgag
     5101 cgattggcaa ataagggcac tgttaaaaat tgaagatccg gccttttatc agcataaagg
     5161 ggtcgatctt cggactccgg gtgccggcct gacaactatc actcaaagcc tggtcaaaat
     5221 ttattacttt gatcacttta aacccggttt tgccaagatc aaacaaacgc taatcgccag
     5281 atttgctttg gatccgctgg tttccaagaa cgatcaactc caattattta tcaatcatat
     5341 ttatttggga accgtaaata atcggccggt tgtcggtttc gtgaacgccg ccagggttta
     5401 ctatcaaaaa gaagtgaatc aattaactga aagagactat ttatcgattg tggcaatgat
     5461 taattcttcc agggccttta gtatcagcga taggcctggc gccaacaggg aacggaccga
     5521 aaggttgatc ctggttgttg agggaaaata tataccaacg catttgatgg acatgtacta
     5581 tggcaaactg gatagcgcga cccaaaagct cttagccccg tcttcttatt ttccggggat
     5641 atatgaaaaa aaataaaaac cgtcgctcgg agcaaacgac aaggaagtaa tttcatgaaa
     5701 ccgaacaagg atctcgtctt actgtattta ttcaatttct ttaaaagcct gcagtttttt
     5761 ggggcggtgg cggtcccctt ttacctccac cgggtcggac tcgattatac ccggatgttt
     5821 tttttagaga ccgtattctc atttggcatg atcgtcctgg aaatccctac cggggtcatt
     5881 gccgaccgct gggggcgaaa gctgtcttta gtcttgggca gctgtttctt cggagccgga
     5941 ttcttactct tcggcatcta tacctcttat ttcattctgg tccttgccga aatcatctgc
     6001 gccttaggaa tgaccctctt gagcggcgct gaccgggcca ttttatacga agtcctgaaa
     6061 cgggaaagcg aggaagcccg ggctggtgcg gtaatggccc ggtatgattc tttagggacc
     6121 gccgcaatgt ttctggcgtt tccggccggt tccttatttg tcggaagtaa gcttgttccg
     6181 tacaaaacgg cattgggtct tgttttcgtg gcgactgccg tagccatagc catatccggc
     6241 ttgatcattc tatttgtgaa agagggagtc tacgaaaaaa gcagggcaaa cccatttcgt
     6301 cagggatttg aaggatttcg ctacatcttc gatcatccga agcttagagt attcagtctg
     6361 aattatgcgc tgatatcttc aatgaccttt tttatgttct ggtt
//
LOCUS       DASVMB010000570        14034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_216143,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000570 DASVMB010000000
VERSION     DASVMB010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14034
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216143"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF531_15965"
     CDS             complement(<1..417)
                     /locus_tag="VF531_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715515.1"
                     /translation="MKKGSRKVLAVVLVLAVVISIASIGMTASSGVDHRKTYRINVFT
                     MLGNTAGLQTGWSAKILKDKFNLEMNMIASNLEGGGDAKFATMMASGNLGDIVILGSD
                     SDGKYTDAIKVGFLLDWTKNGRLNKYAPYIVKNFGKV"
     gene            complement(481..1383)
                     /locus_tag="VF531_15970"
     CDS             complement(481..1383)
                     /locus_tag="VF531_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715516.1"
                     /translation="MELRKTTINDKVFNILNVLFFTIITLICIYPFYYLFINTISDND
                     LSSRGLITFFPNGIHFTNYLQVFKIQNLGHAAFISLARTLLGAFLTVIGSALLGFIFT
                     QKEMWGRKFWYRFVIITMYFNAGIIPWFIIMMKFHLTNNFLAYILPFIVQPFFIVLVK
                     TYIESLPASIQESAEIDGAGYLVIFSRIILPLITPILATVAVFSAVNQWNSFSDTLFL
                     MTDERYYTLQFILYRYMNQASLLAHILQSTQGSVMTSDIAHMQTSTSVRMTVSMIIVT
                     PILFVYPFCQRYFVKGIMIGAVKG"
     gene            complement(1445..2389)
                     /locus_tag="VF531_15975"
     CDS             complement(1445..2389)
                     /locus_tag="VF531_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7715517.1"
                     /translation="MSLSSSKILPHEKTSLKKGTRLFLIALPFLLLVLAFSYLPLYGW
                     IYSFYNYKPGFSLGQCEFVGMQWFRSIVANPTTVAEVIRVLKNTLAISTLGILTSVFP
                     VVFAIFLVEIRSPWVKKTVQVLTTLPNFISWVLVYSFAYALFATDSGLLNQMLIKIGL
                     IQQPINFLASDNFTWIKMALWGLWKYLGWNAIMYLAAIVSIDQELYEAARVDGAGRFR
                     LMWHITVPSILPTYFVLLLFQVANFVNNGLDQYFVFQNALNKGHIEVLDLYVYNIGLL
                     GSNFSFATAISMLKSIISIILLFMVNRMSKLVRGETII"
     gene            complement(2750..4396)
                     /locus_tag="VF531_15980"
     CDS             complement(2750..4396)
                     /locus_tag="VF531_15980"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7715518.1"
                     /translation="MLKVLIVEDEKWEREGLLGFLDWHSFGFEMVETACDGIEGLEKA
                     DKIRPDIIITDIKMPGMDGISMSKKIKASMPQVKIMILTGYDDFQLAQEAISFKADAY
                     VLKPLEEEEIIPVFQKIVDECLEEKEKTAREDRIRKQLDENLRIARTKFLNDLINGNL
                     DTERLNQQLSDFGMKITSHDEYTIMIIKIVRTLGQSHGAVNPEDPASAGMDGIISLVG
                     NVMQQHLVFSLSFVKEEQMVFCFSSSRGDNGLLDSEIGEIYRTIHSEQGVLSVIGMGE
                     PAASLNEVSRSYVQARQVIEFGMFWSSFEMIHFYQIDSKRNKFVDNMSEFLIQSDYLS
                     KQLLHSVRTSNRDKLFELLEEMFAYLRKNPGADRDFIVSYLQSIINETNVFLYNLNKG
                     LEHSSVNVCKDQGVQLAGLSSLRSMEQGLTGFFENTLQKINEKKNDKDEQIVNKVVEL
                     IEQRYMTDLGLQIIAREVFLSPNYLGNIFKKYTGKTFNDYLCEYRMTKAKELLKSPDN
                     KVSRVASAVGISNTSYFCTIFKNTYGMAPGEYQKSFLRNS"
     gene            complement(4389..6212)
                     /locus_tag="VF531_15985"
     CDS             complement(4389..6212)
                     /locus_tag="VF531_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7715519.1"
                     /translation="MEKIINFIKNLSVSWKFILAYFSILIVPVVLMGIFLYIQNSNSA
                     INQAKLVMEQNLLQTKASIVHKEKVIENLAQVLSNTKFVNFLNSGYENDISKVEDYQF
                     DFSPAVKSIIQQDNAIYSIRIYMDKVIVTEMLDSYYSIRAMQSPNLFADASRYEPKVY
                     GWKSIHDALPHLLKANAVTPVQVFSYSKGIYLLNYQHREAVLEFEVREDVLFDMLRDP
                     VISKLGKVFIVDSDHRIISNNLPGIFKKDITNSGFNDFETGKKISVVKEINGVKSIVI
                     SIPLEGLRCSIVGIFPVSNFNVAVKKSLINIILMLFISTIILGFIVYFITNALLGRLK
                     KLAKAMRQVREENLDVSVPVKTMDEFGELALSFNHMTRRIHELVEMVYKIQIIEREAE
                     LKAWESQINPHFLYNTLATISWAARKAGSQEIVKISNSLSKFYRLVLSKGNSLIYVRE
                     ELDMVKAFLQIQKIRFEDKFDVEYVIDENILDCRIIKNILQPLVENALNHGIEPKRMH
                     GTIVIKAWYDPDKLCFQIIDDGVGMDSSTLNDIRAGKIEKSGGSGYAIKNIMERLKAY
                     YRTGQTFDVFSRPGIGTVVTITICTNDSLSEEKAKAGGFYA"
     gene            6991..7464
                     /locus_tag="VF531_15990"
     CDS             6991..7464
                     /locus_tag="VF531_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715520.1"
                     /translation="MVRNSGFEHYKPSPPVCGEPVNFDYWKSDGAVGVTTKPEDVHTF
                     CAAAVLGDCGEISQVIEVKSNLDCHFLKFSFFAKSLCPEGSLIAELLVFDEKHQPIHP
                     CEQKITIKHIPACHYEYYQFVTPESIPENACYIEIVFKNPSTGSKVLLDDVSLSI"
     gene            complement(7591..10701)
                     /locus_tag="VF531_15995"
     CDS             complement(7591..10701)
                     /locus_tag="VF531_15995"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX7715521.1"
                     /translation="MEMNPSTLEITKPEPFYQEPELRPSGIDIIDPVPWGTHFCQFYE
                     TGRDLIETLVPYFREGLEANEFCMWITSPPLEAGEGKTALREAIPDFNSYLAKGQIEI
                     LEYNQWYTPTGRFDAEAVLRGWGDKYAAARQQGYTGLRLSGNTFWLEPAAWGNFANYE
                     AKINEFIDGKRILALCTFSLQRCGVCEILDVISNHHFAIIKRAGDWEKIENSRSRQTE
                     QALRKSEERLDLTVWAADLGIFEWDSSQDRIVWENRRMDAIFGQSLQNEPITTTELLE
                     RMIHPDDVGSFKTELIENMKPGHLVHTICRIRRRGDGELRWIEIFGRFHSDASGNLRL
                     KGGVGDITPRKQVEKALQVKEEELIAAYEELLLQQQELNSTYRDLQRQREEIREHEIA
                     LTVARDEAERHAAELDATISSIAAGVIIYNASGNIIRINEFARILLEISGDVYNLPYQ
                     EFLERFKVRMPDGTPYEVEQTPLSRALRGEIIREEEMMITRNPDKPVWLSATFAPILH
                     NNRTIVGVISIFTDITERKRRVEDLLASERELLRVTLDSLGEGVVAADQEERIIFINE
                     AAVNLSGYSRDDALGELVYKILYLIDDKTSEPIEKIHSRAVYSQLILVTRDLREVMVS
                     INCSPIKAPNGRIIGKVIVVEDISEKQKIEQGLLKTAKLESLGILAGGVAHDFNNILA
                     GILANLQLGLFKLKMGQDISKYLENTIETTRKASGLTKQLLTFAKGGDPVKKSTSITK
                     LMKDAVLFALSGSKVKAKFHLPEDLWIVDVDEGQIIQVINNLTINAEQAMLTGGILEI
                     YGENVIFPEDGNYTPGQYVKLTMKDHGIGIPEEIIDKIFDPFFTTKKTGNGLGLSTSY
                     SIIKKHGGYLEVESAPGIGTTFYILLPAINQELNLKESPRKIEAGGDAKILLMDDEDT
                     IRNIGGEMLSCFGYRVTLAGNGQEAVGLYKQAKEIGEPYDAVIMDLTVPGGLGGIETM
                     AILRQVDPDVKAIISSGYAGNPVMSDYERYGFKGVVIKPYKFDELNKVLHQVLDEKQL
                     PLDLTY"
     gene            complement(10968..13010)
                     /locus_tag="VF531_16000"
     CDS             complement(10968..13010)
                     /locus_tag="VF531_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7715522.1"
                     /translation="MELKEIIEALSLEEKFALLTGKDSWRTVSVERLGIPEIVMSDGP
                     HGLRKVVQDEAGTETTLQAVCFPTSSAISATWNPELARQIGQALGEECGAMGVDILLD
                     PGTNIKRTPLCGRNFEYYSEDPFLAGELAAAYIDGVQSQGVGTSLKHFATNNQEFDRL
                     QISNEVDERALREIYLKPFEIAVKKSQPWTVMCAYNRLNGIYCSENRFLLHDILRDEW
                     GFEGVVVSDWWAVHNRAKALKASLELEMPFAPGSMENLRQAYERGLISDAEIDGALER
                     LLAIVFRAVATREQRAKEYSLEHHHSLAKAAALEAITLLKNEDGLLPIHRERAQRIVI
                     IGEFAEKPAFEGGGSSYVLPRQVDSPLQQIKALAGEAIHIDYFPVLSVAAPQVNQLNL
                     AMAAVRNADLAIVFVGNRNGSEFRGRDGIESEGYDRNYLTLSPEMELVIERISGQNPN
                     TVVVVEAGSAVDMSAWIHQVKAVLFAWYTGQAGGSALAEILFGIENPSGKIAETFPLR
                     LEDTPAYGNYPGNGFVSHYAEGLMVGYRYYDTHQQEVLFPFGFGLSYTAFDYSDLRIA
                     PGSASVNDTVNVSCKVQNTGKLKGKETVQLYIRDTACKVLRPDKELKAFTKVELLPGE
                     QKEIRFELNRDAFAYFSTALNQWHVEEGDFEFLIGASSRDIRLTGKVYIRNGQDFS"
     gene            13283..>14034
                     /locus_tag="VF531_16005"
     CDS             13283..>14034
                     /locus_tag="VF531_16005"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7715523.1"
                     /translation="MNAFLETRSYKSDFAVSAFYASNNNFLAHWHIEAEMMLVCSGSI
                     RIGINKDTRILNQGEMALFRSTDIHYYDSRNLHSSIIVLIFRPELVESPGGWPENRQF
                     DPAFIDERLFAGLAKPVRDEITNLFREIVQELKTQHPHYRLYVKGKIAELCALILRHF
                     PTRPADTPKNGAKKPDILRVQNAIQYLQTHYMEEISLSDIAQACGLSPYYFSRLFSKF
                     TGIPFKEYLTRTRVEKAEQLIRANPRSMIDTA"
BASE COUNT         3782 a         3459 c         2971 g         3822 t
ORIGIN      
        1 tacttttcca aagtttttaa ctatgtatgg agcatatttg ttaagccgac cattttttgt
       61 ccaatcaagg agaaaaccga ctttaatcgc gtcggtatat ttcccgtccg aatcgcttcc
      121 aagaattaca atatccccta aattgccgga ggccatcatg gttgcgaatt tggcatcgcc
      181 tccaccttca agattggaag caatcatgtt catctcgaga ttgaatttgt ctttgagaat
      241 ctttgccgac cagcccgtct gcaaaccggc agtattgccc agcatcgtga agacattaat
      301 tctgtaggtc ttacgatgat ccacacccga ggatgcagtc atgccaattg aggcaatact
      361 gataaccacg gccaggacca atacgacagc caatactttt cttgaccctt ttttcaacac
      421 attttcctcc cttttctctt ttatttaaaa tcccgggtaa tcccggaatt ttaagctgtt
      481 ttatcccttg accgctccga tcatgatacc tttgacaaaa tacctctgac agaaaggata
      541 gacaaacaag atcggagtga cgataatcat ggaaaccgtc atacggacag aggtcgaggt
      601 ttgcatgtgc gcaatatcgg aagtcatgac acttccctga gtactctgta aaatatgagc
      661 caacagggat gcctgattca tatagcgata aagaataaat tgcagtgtat aatagcgttc
      721 atccgtcatc aggaacaaag tgtccgaaaa tgagttccac tgattgacag cggagaatac
      781 ggccacggtt gcgagtatcg gggtaatcaa cggcaaaatg atcctcgaaa atataactaa
      841 ataccccgcg ccgtcaattt cggccgattc ctgaatggat gcaggcaacg actcaatata
      901 ggtttttacc agtacgatga agaaaggctg tacgataaaa gggagaatgt atgccaggaa
      961 gttattggtc aggtgaaatt tcatcatgat gataaaccag ggaatgatgc cggcattaaa
     1021 atacatcgta attatcacaa aacggtacca gaatttcctg ccccacatct ctttctgggt
     1081 aaatatgaat ccgagtaaag cggaaccaat gactgtcaaa aatgcgccaa gcaatgtcct
     1141 ggcgagggag ataaatgcgg cgtgccccaa attctgtatt ttaaacacct gaaggtaatt
     1201 ggtaaaatgg atcccattcg ggaaaaaagt gatcagccct ctactgctca aatcattgtc
     1261 gctgatcgtg ttgataaaga ggtaatagaa agggtagata caaatcaatg taataatcgt
     1321 aaaaaaaagg acgttcagta tattaaaaac cttatcgttg atagttgttt ttcgcagttc
     1381 cattcgaact cctccgccga aaatgaatta ttcacgtgca cgcgacgcgt gcgcgaggct
     1441 agcgtcagat aatggtttcg ccgcgtacca gcttagacat gcggttgacc ataaagagca
     1501 ggataatgct aatgatggat ttcaacatgc ttatcgcggt ggcaaatgaa aagttggacc
     1561 cgagtaatcc gatattgtag acatagagat caagcacttc aatatgccct ttatttaacg
     1621 cgttttgaaa gacgaaatat tgatccagtc cattgttgac aaagtttgcc acctggaata
     1681 acaagagaac gaaataagtc ggcaggatac tgggaacggt aatatgccac attaaccgga
     1741 atcgtccggc gccatccact cgggcggctt cataaagctc ctgatcgatg ctgacgatgg
     1801 cggccaaata catgatagcg ttccagccga ggtatttcca caaaccccat aaagccatct
     1861 tgatccaggt gaaattgtcc gaagccagaa agttaatagg ttgctggatt agtccgattt
     1921 tgattaacat ctggtttaac agtccgctgt ccgtcgcaaa aagggcgtaa gcaaacgaat
     1981 ataccagtac ccagctaatg aagttcggta aggtagtcaa cacctgtacg gtttttttaa
     2041 cccacggact tctgatttcc accaaaaata tggcaaagac aaccgggaag acggaagtaa
     2101 ggatgccaag ggtacttatg gccaaagtat tcttcaggac tcggatgact tccgctactg
     2161 tggttggatt ggcaacgatg gacctaaacc attgcatccc tacaaattca cattggccga
     2221 gactaaaccc tggtttataa ttgtagaagg aataaatcca accatacaaa gggagataag
     2281 aaaaggcaag caccagcagc agaaaaggaa gcgcaatcag gaacaagcgc gtacctttct
     2341 ttagtgacgt cttctcatgc ggtaagattt tacttgacga taagctcatt aaattgcccc
     2401 caatatctgt ccaaataaga aaaaaactta cacatatatt gtaagtttaa gaaccaaaga
     2461 tgtatatgac aaaaatcaat gatgatttca attattaacg caaatgtaac ggtggagaac
     2521 taaaacagtg tacctatggg gttctaggca acaggcaata ggcaacagaa aaaactgaaa
     2581 agagaaaagg aatttgttcg cccttattag ggtgagctaa aaattttaag cacaagtacg
     2641 tatcaccctc catctgagta agctttactg acggcttccc aatgggggga gagccgaccc
     2701 tggtgttgac tgaggtaaat ccattgcgcc agttccctat tgcctgttgt tatgaatttc
     2761 tcaaaaagct tttctgatac tccccggggg ccattccata ggtgttcttg aagatggtgc
     2821 aaaaataaga tgtgtttgag ataccgactg cggaggccac ccggctcacc ttgttatccg
     2881 gtgactttaa gagctctttt gctttcgtca ttctgtactc acagaggtaa tcattgaaag
     2941 ttttaccggt gtacttttta aaaatgttcc cgagataatt cggagacaaa aacacttccc
     3001 tggcaatgat ttgtaaaccc aaatccgtca tatatctttg ttcaatcaac tcaaccacct
     3061 tgttgacgat ctgttcatcc ttgtcgtttt tcttctcgtt tatcttttga agcgtatttt
     3121 caaaaaaacc tgttaatccc tgctccatcg accgcagact cgataaacct gctaattgta
     3181 ctccctggtc cttacagaca ttgacacttg aatgctccaa acctttattc aggttataaa
     3241 gaaacacatt tgtttcattg attatgcttt gaagataact gacaataaaa tccctgtccg
     3301 cccccggatt tttccttagg tacgcaaaca tctcctccaa aagctcaaac aacttgtctc
     3361 gattcgaagt tcgtaccgaa tggagcaatt gtttggataa gtagtcgctc tgaatgagaa
     3421 attcgctcat gttgtcaaca aacttattcc gcttggaatc gatctgatag aaatgaatca
     3481 tttcaaaaga tgaccaaaac atcccgaatt caatgacttg tctggcctgg acgtaggagc
     3541 ggctgacttc gttcaaggag gctgccggtt cccccatacc aattacggat agtactccct
     3601 gttcactatg gattgtacgg tatatctctc caatttccga atccaataga ccgttatctc
     3661 cccgggaact ggaaaaacaa aaaaccatct gttcttcctt aacaaatgac agagaaaata
     3721 ccagatgttg ttgcattacg tttccaacca gcgatatgat tccatccatt cccgcagatg
     3781 cggggtcttc agggttaacc gccccatgac tctgtcccag ggtcctgacg attttaataa
     3841 tcataattgt atattcatca tggctagtga tcttcatgcc aaaatcggat aattgttgat
     3901 taagccgctc cgtatcaagg ttcccgttaa tcagatcatt taaaaacttt gttctcgcga
     3961 tacgaagatt ttcgtccaac tgctttctga ttcgatcttc ccgggcggtt ttttcttttt
     4021 cttcgaggca ttcatcgaca atcttttgaa aaaccggtat gatttcttcc tcttccaggg
     4081 gcttcagaac ataggcatcg gccttaaagc tgatcgcctc ctgggcaagc tggaaatcgt
     4141 cgtaaccggt taaaatcatt attttcactt gcggcatcga tgcctttatt ttcttggaca
     4201 tactaatgcc gtccattccg ggcattttta tatctgtgat gatgatatcg ggacggattt
     4261 tatcggcttt ttccagccct tcaataccgt cgcaagctgt ttcaaccatt tcaaatccaa
     4321 aactatgcca gtcaaggaac cccaatagcc cttcccgttc ccatttttca tcctccacga
     4381 tcaacacttt aagcataaaa accacccgct ttggcttttt cctcagatag tgaatcatta
     4441 gtgcagatgg ttatggttac tacggttcca atgcccggtc tgctgaaaac atcgaatgtc
     4501 tggcctgttc ggtaatatgc cttcagcctt tccatgatgt ttttgatcgc atatccgctc
     4561 cccccggact tttcgatctt acctgcccgg atatcattca gggtagagct atccatgccg
     4621 acaccatcgt caatgatctg aaaacacagc ttatccggat cataccaggc cttgatgaca
     4681 atcgtaccat gcattctttt cggttcgatc ccgtgattta aagcattctc gaccagaggt
     4741 tggagaatgt tcttaataat cctacagtcg aggatgtttt catcaatcac atactccaca
     4801 tcaaacttat cctcgaaccg aatcttttga atctgcagaa acgccttaac catatccagt
     4861 tcttcccgga cataaataag gctgttgccc ttgctcaata ccagccgata gaatttggag
     4921 agggaattgg atatttttac aatctcctgt gaccccgctt ttctggcggc ccatgaaatc
     4981 gtagcaaggg tattgtaaag aaaatgtgga tttatctggg attcccatgc cttaagctcc
     5041 gcttcccttt cgataatctg aattttatat accatttcaa caagttcatg gatccgcctg
     5101 gtcatatggt tgaagctgag cgccagttct ccgaattcat ccatggtctt tacaggtacg
     5161 gaaacgtcca gattctcctc tctgacctgc cgcattgcct tggctaattt ctttaacctg
     5221 ccaagaagcg cattggtgat gaaataaacg ataaacccca gaataattgt ggagataaaa
     5281 agcatgagaa tgatattgat caacgacttt tttacagcga cgttaaagtt gctgacggga
     5341 aatattccta caatactgca acgaaggcct tcgagaggta ttgagataac tatcgacttc
     5401 accccgttta tttcttttac aacactaatc tttttcccgg tttcaaaatc attaaagccg
     5461 ctgttcgtga tatccttttt aaagatccca ggtaagttat tggatataat gcggtgatcg
     5521 gagtcaacaa taaacacctt gccgagcttg ctgataacgg ggtcgcgtag catatcaaag
     5581 agtacgtctt ccctgacttc aaactccagt acagcctccc tatgctgata gtttagcaag
     5641 tatatgccct tgctgtatga gaaaacctgc accggagtga ctgcattcgc cttaagtaaa
     5701 tgcggcaaag catcatggat gcttttccag ccatacactt tcggttcgta tctcgatgca
     5761 tcggcaaaca aattaggaga ttgcatcgcc ctgatactat agtaactgtc cagcatttca
     5821 gtgacgatta ccttgtccat gtatatccgg attgagtaaa tcgcattatc ttgctggata
     5881 atcgatttaa cagcagggga aaagtcaaat tgataatctt ccactttgga gatgtcattt
     5941 tcgtatccgg agttcaaaaa gtttacaaat ttcgtattcg ataatacctg agccaaattt
     6001 tcaatgactt tttccttatg aacaatgctg gctttggttt gcaacaggtt ctgctccatg
     6061 accagctttg cctggttaat cgcagaattg gagttctgta tgtacagaaa aatccccatt
     6121 aataccacag gtacaataag aatgctaaaa tacgccaaga taaatttcca gcttacactg
     6181 agattcttga tgaaattgat gattttttcc atttttatct caaaccccgg tttttgttca
     6241 caatatattc tccataaaat attataaacc taatagacca aattagtaag atttcatcaa
     6301 tatataatat attgttcggc caaaagctaa attcttcttt accgaaaaca aatacccttc
     6361 ctggaccttt tcttccccca atcaatccat agcaccggcc aatggatcgt ttggcatggt
     6421 tatctaactg aattgccata taactgggac cttcacactg ttaacgttgg gagaatagaa
     6481 tattttagcc cggttgaagg agcgttagat aaaggagcgt ttggtatgat agtaataata
     6541 aacaaggcgc ttcaattggc ggaactggtt tgcggaaagg tacggggcct tcctttgccc
     6601 gcctttaaga agcagccttt aatggatgat tgtgagggag cattagcggc tttacaaaga
     6661 ggtgattttg gagtcgcgct gtttaatgtt aatgttttga atgataagct acatacactc
     6721 cgcgagaagg gactctgtct ggctacagcc cctttatttg cggatattca tcatttacaa
     6781 cagttattgt taaaacttcc tcttaagaag gaaggaccgc cgggacccat gggacctcag
     6841 ggaccaacgg ggccaccggg accgtcagga ccccccggac cgccgggacc cacgggaccg
     6901 tcaggacctc caggacccac gggaccgccg ggcaaacaag gaccaacggg acccccttgt
     6961 ccaaagattc acaaaaggaa actcggggga ttggttcgta attcgggatt tgagcactac
     7021 aaaccatctc ctcccgtttg cggggaaccg gtaaactttg attattggaa aagtgatggt
     7081 gctgtcggag taaccactaa acccgaggat gtacatacat tttgcgccgc ggcggttttg
     7141 ggagattgcg gtgaaattag ccaagtcatt gaagtcaaat ccaacttaga ttgtcatttc
     7201 ttgaaattca gttttttcgc caagtctctt tgtcccgaag gatctctgat tgcagaactg
     7261 ttggtttttg atgagaagca ccaaccgatt catccttgtg agcaaaaaat cacgattaag
     7321 catataccgg cttgtcatta cgaatactat caattcgtga ctccagaatc tatcccggaa
     7381 aatgcatgtt atattgagat cgtttttaaa aacccgtcca ccgggagcaa agtattactg
     7441 gatgatgtct ccttaagtat ttaaaggtgc ctgtttcaag ggactatttc ttcaagaaac
     7501 tcttatccca atcgcaagcc ctgacttttc aaagtcaggg cttttcctta agcccaatat
     7561 cgatttaacc tgcccctaca aaatatggcc tcaatacgtc agatcaagcg gtagttgttt
     7621 ttcgtcgagc acctgatgta aaaccttatt cagttcatca aatttatacg gtttaatgac
     7681 aactccctta aaaccgtacc tttcatagtc ggacatcacc ggattgccgg cataaccgct
     7741 ggatataatg gcttttacat ccggatcaac ctggcgcagg atggccattg tctcgatgcc
     7801 tcccaacccg cctggtacgg tcagatccat tatcaccgcg tcatatggct caccaatctc
     7861 cttggcctgt ttataaagtc caaccgcttc ctgcccgttt ccggccaatg taacccggta
     7921 cccgaaacag cttaacatct caccgccgat gttgcggatg gtatcctcat catccatcag
     7981 taatattttg gcgtcccctc cggcctcgat cttccttggg gactccttaa ggttcaattc
     8041 ctgattgatc gccggaagca agatataaaa cgtggtgccg attcccggtg ctgattccac
     8101 ttccaggtaa ccaccatgtt ttttgataat ggagtaagaa gtcgacagac ccaaaccgtt
     8161 acccgttttt ttggtcgtaa agaacggatc aaaaattttg tcaatgattt cttcgggaat
     8221 cccgatacca tgatccttca tggtcaattt tacatattga ccgggagtat aattcccatc
     8281 ttctgggaat attacattct caccgtagat ttcaaggatg cctccggtaa gcatcgcctg
     8341 ttcggcattg atggtcagat tgttaataac ctgaatgatt tgaccttcat caacatccac
     8401 gatccaaaga tcctccggaa gatgaaactt cgcttttact ttggaaccgc ttaaggcaaa
     8461 caaaacggca tccttcatca atttcgtaat ggaagtcgac ttcttaacgg gatcgccgcc
     8521 cttagcgaag gtaagcagtt gcttagtgag cccgcttgcc ttacgggtcg tctcgatcgt
     8581 attttccaaa tacttcgaaa tatcctgccc catttttaac ttaaacaatc ccagttgcaa
     8641 attggccaag atccccgcca gaatattatt gaagtcatgg gccacgccac cggcaagaat
     8701 acccagtgat tcaagcttcg ccgttttcaa cagaccctgt tcgatctttt gtttttcgga
     8761 gatatcctca acaacaatca cctttccaat aatccgccca ttcggtgcct taatgggaga
     8821 gcagttgatg gagaccatga cttctctcaa atcccgggtt accaaaataa gctggctgta
     8881 taccgcgcgg gaatggatct tttcaatcgg ttcactggtt ttatcatcga tgaggtaaag
     8941 aattttataa accagttctc caagggcatc gtcccgggaa tatccgctta aattaactgc
     9001 ggcttcattg atgaatataa tccgttcttc ctggtccgca gcaactactc cctcgccgag
     9061 ggaatccagg gtcaccctca aaagctctct ttccgaagcc aggagatctt ccaccctgcg
     9121 tttccgttcg gtgatatcgg tgaatatgga tatgacgccg acgatagtcc gattattatg
     9181 gagaatcggt gcaaatgtag ccgaaagcca taccggttta tccggattcc tggtaatcat
     9241 catctcttct tcacggataa tttcaccccg tagcgccctg gatagaggag tctgctcgac
     9301 ttcataagga gtaccgtccg gcatacgcac cttaaatctt tccaaaaact cctgataagg
     9361 caaattgtaa acatcgccgg aaatctccag caaaatacgg gcaaattcat taatccggat
     9421 gatgtttccc gatgcgttat aaataatgac acccgccgca atcgaggaaa tcgtcgcatc
     9481 cagttctgcc gcatgccgct ccgcttcatc gcgtgccacg gttaaggcaa tctcgtgttc
     9541 ccggatctct tccctttgac gctgcagatc acgataggtt gaattcaact cttgttgctg
     9601 gagtaataac tcttcgtagg ccgcaatcaa ctcctcttcc ttcacttgca acgccttttc
     9661 aacctgtttg cgcggggtga tatctcccac tccccccttt aaccgcaagt taccgctggc
     9721 gtcactgtga aaacgtccga aaatttcaat ccaacgtagt tcaccgtcgc ctcggcgccg
     9781 aatgcggcag attgtgtgaa ccaggtgtcc cggcttcatg ttttcaatta gttccgtctt
     9841 gaaggaaccg acatcgtccg ggtgaatcat ccgctccaag agttccgtcg tggtgatcgg
     9901 ttcattctga aggctctgcc caaagatcgc atccattcgc cggttctccc agacgatacg
     9961 atcttggcta gagtcccatt caaatatacc cagatccgcc gcccaaacag tcaagtccag
    10021 tcgctcttca ctcttccgta aagcctgttc ggtttgtctg gatctggagt tctcgatctt
    10081 ctcccaatcc ccggcccgtt taattatggc aaaatggtga ttactgatta cgtccaggat
    10141 ctcgcaaacg ccgcacctct gcagtgaaaa agtacaaaga gccagtattc tcttgccatc
    10201 gatgaactca ttgatcttag cttcataatt ggcaaaattg ccccaggccg caggttccag
    10261 ccagaaagta ttgccggaaa gacgcaagcc cgtatagccc tgttgccggg cagccgcata
    10321 tttatctccc catccccgga gcactgcctc ggcgtcaaac cgtcctgtcg gcgtatacca
    10381 ctgattatat tccaagattt cgatttggcc cttggccaga taactgttga aatcaggtat
    10441 cgcttctcgc aacgcagtct ttccttctcc cgcttccagc ggaggggagg taatccacat
    10501 gcaaaattcg ttcgcttcca gtccttcgcg gaaataaggc accagtgttt cgataaggtc
    10561 ccggcccgtc tcatagaact gacaaaaatg cgtaccccac ggcactggat caatgatatc
    10621 aatcccggat ggacggagtt ccggttcttg gtaaaacggt tcgggttttg taatctccaa
    10681 tgttgatggg ttcatttcca ttggtcacac cccagataat cttttttaac cgtaattgat
    10741 gtgatatacc ccatttgttt catgcaatga aacgagcgtt ggtctgatca aattatgatt
    10801 tgatgtataa atggattttc ctgtatcaat cggtaatcta ttcaaaattt attatagcaa
    10861 atgttgtttt ccaaaacgtc gcttacgatc gtgatgaaat atttaccaat aaaaaaaacg
    10921 ccccagtatg gctgaggcgt cttttcattt atactaagct ttatgattta agaaaaatcc
    10981 tgcccgttcc ggatatagac cttgccggtc aaccggatat cccgggaaga agccccgatt
    11041 aaaaactcaa aatccccctc ttccacatgc cattgattga gcgcggtact aaagtaagca
    11101 aaggcgtccc ggttcagttc gaagcggatc tccttttgct cgcccggcaa cagctccacc
    11161 ttggtaaagg ctttaagttc cttgtcggga cgtagaacct tgcaagccgt gtcccggatg
    11221 taaagttgca cggtttcctt cccttttagc ttgcccgtat tttgcacctt gcagctgacg
    11281 ttcaccgtgt cattgacgga ggcgctcccc ggcgcaatcc gcaaatcgga gtagtcgaac
    11341 gcggtatagg aaagaccgaa cccgaaaggg aagagtacct cctgttgatg ggtatcgtaa
    11401 taacggtagc ccaccattaa cccctcggcg taatgggaga caaaaccatt gcccggataa
    11461 ttgccatagg ccggtgtgtc ctccagacga agcgggaagg tctcggcgat cttgccactg
    11521 ggattctcga ttccaaacag gatttctgcc agcgcgctcc cgccggcctg tccggtgtac
    11581 catgcgaaca acaccgcctt cacttgatgg atccatgcgg acatatccac ggcggaaccc
    11641 gcctctacaa caaccaccgt attcggattt tgaccggaaa tacgctctat aaccagttcc
    11701 atctcaggag aaagggtgag gtaattccga tcgtacccct cggattcaat accatccctg
    11761 ccccggaact ccgaaccatt acggttaccg acgaatacga tagccagatc ggcgttccgt
    11821 acagcagcca tcgccaggtt cagctgatta acctggggtg ccgctacgga gagtaccggg
    11881 aagtaatcga tatgaatcgc ttctccggct agggccttga tctgttgtaa cggactgtcg
    11941 acctgtctcg gaagcacata ggagcttcca ccgccttcaa aggccggctt ttcggcaaat
    12001 tcaccgatga tcacaatccg ttgggcgcgc tcgcgatgga tcggcaacaa tccatcctca
    12061 ttcttcaata atgtgatcgc ttcaagcgcc gcagccttag ccagggaatg gtggtgttcc
    12121 aggctatatt cctttgcccg ctgctcacgg gttgctacag cccggaagac gatggcgagt
    12181 aaccgttcca aagccccatc gatctcggcg tcactgatca agccgcgttc ataggcttgc
    12241 cgtagattct ccatgctccc cggagcaaag ggcatctcca gctccaagct agccttgaga
    12301 gccttggccc ggttgtgaac ggcccaccag tcggaaacca ccacgccttc aaaaccccat
    12361 tcatcccgaa ggatgtcatg gagaaggaaa cggttctcgc tgcaataaat gccgttcaga
    12421 cgattatagg cgcacattac cgtccaaggc tgtgattttt tcacggcaat ctcaaacggt
    12481 ttcaaataaa tctcccttaa tgcgcgttca tccacttcat tgctgatctg gagcctgtcg
    12541 aactcctgat tgttggtagc gaaatgcttg agagaagtac ccacaccctg gctttgcacc
    12601 ccgtcgatat aagctgctgc cagctcacct gcaagaaagg ggtcttccga gtagtattcg
    12661 aaattgcggc cacagagcgg agttcgtttg atattggttc ccggatccaa caggatgtcc
    12721 actcccatcg cgccgcactc ttcacccagg gcttggccga tctggcgcgc caactcagga
    12781 ttccaggtag cggagatggc tgaagatgtt gggaaacaaa ccgcctggag ggtggtttca
    12841 gtgccggctt catcctgcac taccttgcgg aggccgtggg gcccatccga catgactatc
    12901 tcgggaatcc ccaaccgttc gacggaaacg gtccgccaac tatctttacc ggtaaggagt
    12961 gcaaatttct cttccaagct taatgcctcg attatctctt tcaattccat gataacacct
    13021 ctttggaagc agtatcgaat gcctattaaa attatactgc aacggcccgg atgattctgg
    13081 ccaaaatcta ccacggaata gacagttttt gctctttgaa atataatgat gatattggga
    13141 taaaaagtat tgattctctt ccggtctttc aaacaccggc tttgaaggga ttatcaattt
    13201 ataatttggc cggagagcag tttgagattg gtcaaaattg catctatgcc ccggcaacca
    13261 attgatgagg cgaggcgcag caatgaatgc cttcttggaa acacgcagct ataaatcgga
    13321 ctttgcggtc agtgcctttt atgcttcaaa taataatttc ctggcccact ggcacattga
    13381 ggcggaaatg atgctggtct gctcgggaag tattcgaatc ggcatcaata aggatacgcg
    13441 aatcctgaat cagggggaga tggcgctatt ccggagcacc gatatccatt actacgatag
    13501 ccgtaatttg cactcctcca tcatcgtatt aatcttccgc ccggaactgg tggaaagtcc
    13561 cggaggctgg ccggagaacc ggcaatttga ccccgccttt atcgatgaaa gattattcgc
    13621 cggcctggcg aaaccggtcc gggatgaaat aaccaacttg ttccgggaga tcgtccagga
    13681 gctaaagact caacatcctc attaccgtct ttatgtcaaa ggaaagatcg ccgaactctg
    13741 cgccctaatc ttgcgccatt ttcccacccg cccggcggat actcccaaaa acggcgccaa
    13801 aaaaccggat attctgagag ttcaaaatgc gattcaatat ttacagaccc actatatgga
    13861 ggagatctcc ctatccgata tcgcgcaggc ctgcggtctt agtccttatt atttttcacg
    13921 tttgttctcc aaatttaccg ggattccttt caaggagtat ctcacccgga cccgggtgga
    13981 aaaggcggag caactgattc gggctaatcc gaggtcgatg attgataccg ctat
//
LOCUS       DASVMB010000571         8937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_510312,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000571 DASVMB010000000
VERSION     DASVMB010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510312"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VF531_16010"
     CDS             complement(<1..801)
                     /locus_tag="VF531_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014371196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715524.1"
                     /translation="MKAYLSVFRVRLLVGLQYRVAALAGVATQFFWGFMFIMIYQAFY
                     LNSTKAQPITLEQLISYIWLQQAFLALTMLWYRDNEIFNLITGGNIAYELCRPWSLYG
                     FWYAKLLAQRLSGALLRCFPILIIAFILPEPYRMSLPPNFSSLGLFLITLCLGVLVLV
                     AISMFIYISVIVTMSPAGSLLMFGVFGEFFSGAIIPVPLMPLWLQRITYVLPFRLAAD
                     LPFRVYSGNIASKEAFIGIMVQILWLTILVLVGRITLKKLLRRIVVQGG"
     gene            complement(798..1775)
                     /locus_tag="VF531_16015"
     CDS             complement(798..1775)
                     /locus_tag="VF531_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7715525.1"
                     /translation="MITVEGLCKTFKVPKRSAGLKAAVKSLVYREYTEVEALCEVSFH
                     IAAGEIVGYIGPNGAGKSTTIKVMSGILVPDKGRCHIMGRTPWKERIRHVKNIGVVFG
                     QRSQLWWDVPVIDSFELLKDIYKIPQGEYRRTMTMLVDTLGLQNIINNPVRGLSLGQR
                     MRCEIAASLLHNPKILFLDEPTIGLDAVSKIAVRQFIKRINQEQKVTVILTTHDMNDI
                     EALADRILLIGKGRILYDGSLDTLRSRFGSNKTITVEFAEHRKLPNIHGTKILFCSDG
                     RAGLDVDTRKIKVSEVIAALSEQMELLDVTVENPPIEEMVVQLYQEYQI"
     gene            2059..2415
                     /locus_tag="VF531_16020"
     CDS             2059..2415
                     /locus_tag="VF531_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7715526.1"
                     /translation="MDAFNGAQGRILFILWQNDNLPINELGQKTSLSKSTLTSMLDRM
                     EQQGNVRRNYDPNDRRQIRITLTENSRALNQKYLEVSQKMNQIFYQGFSDAEIVAFEK
                     TLTGILNHLKQYEKGQ"
     gene            2473..2892
                     /locus_tag="VF531_16025"
     CDS             2473..2892
                     /locus_tag="VF531_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7715527.1"
                     /translation="MDGKFIKEINRLANAKNIIVSSVDGQGYPNSKAMFKVAHDGLQT
                     FYLSTNTSSMRVQQFLKNPKASLYFFDRIKVHGLMLVGEMEVLTDDATKRRFWKPFWK
                     IYYPKGVTDPDYAILRFTAKSGNYYRSLQKYRFEIDE"
     gene            complement(2980..4005)
                     /locus_tag="VF531_16030"
     CDS             complement(2980..4005)
                     /locus_tag="VF531_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7715528.1"
                     /translation="MSKLKFAIIGAGVWGETHAYLYQEHPLVDLVAICDLNKARAEKL
                     AQKFGIKQVYSDHRQMLKEVDLDAVAIVTPDFAHAQLAVDCANAGKHILLEKPLATTR
                     EDVEAIVNAVKSNIVRLMVDLHNRWNPPIAVAKQALDDGELGDPVSAYYRLNDIKWVA
                     TDMLPWAAKSSILWFLGSHAVDTVRWFMNDEVETVYAASSSGVLKKLGVDTTDIYQYI
                     LEFRHGGIASLENGWITPNTYPCVNDIKFNITGTKGMMNLDLSNSQMIERFTDEKNDR
                     PDVLVRHFVHGKAKGFAYESIRHFVDKLASGEKFLVSVEDAANTSLVILAIMESARTG
                     KPVKVQY"
     gene            complement(4019..4834)
                     /locus_tag="VF531_16035"
     CDS             complement(4019..4834)
                     /locus_tag="VF531_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715529.1"
                     /translation="MKKIKQLLWKLALVLVCIIAVFPFFWMLLVSLKTRVLTYDPSVW
                     IFTPTLENYLALFQQRHIMLFVRNSLIVVICTTVISLILGSMAAYGFARFSFSKKEDV
                     AFWILGLRMLPPMATVIPFFILARFTGLLDTHLILILAYMTFNIPFTVWMMRGFFEEL
                     PAQIEESALVDGCSHWQALTKILLPLTLPGLTATAIFCIINSWNEFAFALFLTGVNAR
                     TLPTTVTFFLSITGVIWGEMAAVGVLIALPVLIFAMLVQRYMIRGLTFGAVKE"
     gene            complement(4831..5823)
                     /locus_tag="VF531_16040"
     CDS             complement(4831..5823)
                     /locus_tag="VF531_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715530.1"
                     /translation="MGLLLPIIHQYGILLGQYIEIGREVQFIDLSNSQKRSWFARNPH
                     WAYILPSVIILVLLTIVPMIFLYYVSVTNYELGQFWNDIHVVGLKNYIRLFSGRDNEF
                     WSSVGISLLFMFLATFFETICGFIVALLLNHKFKLKSLVFACLLVPITITPSVVGQMW
                     KLMYNAEYGVINYFIGLLFHTKVVWLGPALAYTSTLLVDIWQWTPFMALIIYAGLTSV
                     PQEPYEAALVDGAGVFQSFKYVTLPLLRPLLVLAILFRSMDSLKLFDVPFVLTQGGPG
                     SATELLSLHIYNLGFAQTGWIGRASATGVVLLIIITVLSQFLVRQLHKEGETKI"
     gene            complement(5851..7170)
                     /locus_tag="VF531_16045"
     CDS             complement(5851..7170)
                     /locus_tag="VF531_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7715531.1"
                     /translation="MKKGFAVLLVGMLALSNAVFAKTDSLDQWAQAIKAKYNGTTINV
                     AFASHPSTEAFQKMTPEFEKLTGIKVKWDVMEETYLKNKQLMDYTGNSHLYDVLMVDG
                     FWMSEYAPKKVIAPLKSFINSKTTPGWFDYKDIVPAYSSGIGTYQGQIYGIPTAGETR
                     FVGYRKDLFAKYGKRPPKTMDEMLELAKFFNGKEPGLYGIAMRAQRGIHFASGWMTVM
                     YNFGGGFVNQKTWKVTMTDHRTVQSLKYFVALERNAPPDVASYTHEEAVSAFMAGKTA
                     MWFDATALVPWITDPKKSTIYDKVGFVAPPKGPAGDAGVLAGWNVAIASNSRNKEAAW
                     AFITYMTSKLKAKEYVQNGGVPTRTSVYSDKELVAKDPSYPVQLQALSCASALVKKGI
                     AWLPPTSKLDKILDRAGYYGNLALTGEITPEDACKKAQVEIEDIMSE"
     gene            complement(7228..8442)
                     /locus_tag="VF531_16050"
     CDS             complement(7228..8442)
                     /locus_tag="VF531_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7715532.1"
                     /translation="MRANNAWVKGQNITLVLNLVREMRSISRAEISKKTGLSRSTVSQ
                     VINRLLEAGIIEETGSGESIGGRRPIILRIRPEGRWVCAAHVDDNGQIHMRVEDLIGN
                     EVKRSSGLVKKPDELVPVLTNMIKDLSGKELNRIVCITLALPGIISLDGCILSAVNLG
                     WKSVPVGSQLTKNINIPVLAENATGLAAYGELDIRAINSKNLVYVRIESAVGAGIVTD
                     HKLYHGLRGSEVEIGHMVVDMEGKPCKCGRKGCLETRVSRAAVCEILTDICKVKSLEA
                     PDIDENNIFEWLVAQDNEENLLAQGILKTVARNISVAIVNVLNLMGADTIVIQSKLCD
                     SKTFWMELNQLVFKEVLPFARGGFEIMRSISGEDAILQGATAYARQYFFEKVCYKSLL
                     SAESADAKEVDQ"
     gene            complement(8701..>8937)
                     /locus_tag="VF531_16055"
     CDS             complement(8701..>8937)
                     /locus_tag="VF531_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7715533.1"
                     /translation="MMNGYSESKKQLQQYAREYSMAVVMANYNAPTGKYHPVGKSAVW
                     DNSGNSLASAAKKEDALVLLIRQRNGWTGKVMRL"
BASE COUNT         2595 a         2223 c         1789 g         2330 t
ORIGIN      
        1 gcccccttgc actacgatcc ggcgtaagag ttttttgagg gtgattctcc ctaccaaaac
       61 caggatagtt agccaaagaa tctgtaccat aattccaata aaagcttcct ttgacgcaat
      121 attaccgcta tataccctga aaggcagatc ggcagccaac cgaaacggga gcacataagt
      181 gattcgctgt agccagagcg gcatcagggg taccggaata atcgccccgg agaaaaattc
      241 tccgaaaacg ccgaacataa gtaacgatcc tgccggcgac atggtgacga tcactgagat
      301 atagatgaac atggatattg ccacgagcac aagcactccc aggcagagag tgatcagaaa
      361 taacccgagt gagctaaaat ttggcggcaa agacatgcga taaggctccg gtaaaataaa
      421 cgcgatgatg aggatcggga aacaacgcaa taatgccccg gataatctct gagccaacaa
      481 cttggcatac cagaaaccgt ataaactcca gggacggcat aattcgtaag caatattgcc
      541 gccggtaatc aggttaaata tctcgttatc ccgataccaa agcatagtca aggccaaaaa
      601 agcctgttgc agccagatat aggatatcag ttgttccaaa gtaatcggtt gagccttggt
      661 ggaatttaga tagaacgctt gataaatcat gataaacatg aatccccaaa aaaactgcgt
      721 cgccacaccg gcaagggcag ccactcgata ttgcagaccg actaacaagc gaacccgaaa
      781 tacggaaaga tatgctttca tatctgatac tcctgatata actgaaccac catctcctcg
      841 atgggcgggt tctccaccgt gacatccaat aattccatct gctccgagag tgcggcgata
      901 acctccgaaa ctttaatctt ccgggtatcc acatctagac cggcccgccc atcggaacaa
      961 aaaaggattt tggttccgtg aatattgggg agtttacgat gttccgcaaa ttcaacagta
     1021 attgttttat tggaaccaaa gcggcttcgc agggtatcca ggctgccgtc gtacaggatt
     1081 cttcccttcc caatcaatag aatccggtcc gccagggcct cgatatcatt catatcgtgg
     1141 gtggtgagga tcacagtaac tttctgttcc tgattgatcc gcttaatgaa ttgccgcacg
     1201 gctatcttgg aaaccgcatc caagccgatg gtgggttcgt ccaaaaaaag gatcttcgga
     1261 ttatgcaaca acgaggctgc aatctcacac cgcatccttt ggccgaggct gagcccgcgg
     1321 acgggattat tgatgatgtt ttggagaccg agcgtatcga ccagcatcgt catggtacgg
     1381 cgatactctc cctgaggtat tttatagata tccttgagga gttcaaagga gtcgatcacc
     1441 ggaacatccc accagagctg cgacctttgg ccgaaaacaa ctccgatatt tttgacgtga
     1501 cgaatccgtt ccttccaagg tgtacggccc ataatgtggc atcggccctt atccggaact
     1561 aaaataccgc tcatcacctt aatggtggtg gattttccgg ctccattcgg cccgatatag
     1621 ccaacgattt cacccgcagc gatatggaac gatacttcac ataacgcctc aacctcagtg
     1681 tattcacgat acaccaatga ttttaccgca gccttgagcc cggcggaacg cttcgggact
     1741 ttaaaagtct tgcataagcc ctcgacggtt atcatctcaa cctccagttt ttgtttacgg
     1801 gaactatatt ttacactatc tattgcaaaa aaggaagcat ttaacaaaga tgaatccccc
     1861 caaaaagctt gggatatcat taataataaa ttttaaattc atcaggtatt gaccaaaatt
     1921 gtactatata gtactattct atatagaact ttatgaaagg ggatatccat catcgaaagg
     1981 acgcaaggcg gttttctaat ctctcagatc aaacagatcc agagtcggat ctttgagaaa
     2041 ctcctccggg aaaacgggat tgacgccttt aacggcgccc agggcaggat tcttttcatt
     2101 ctttggcaga acgataatct acccatcaac gaactcgggc aaaaaacctc cctatccaag
     2161 tccacgctca ccagcatgct cgaccggatg gaacaacaag gcaacgtccg aagaaactat
     2221 gatcccaacg atcggcgcca gatacggatt accttgacgg agaactccag ggcgctcaat
     2281 cagaaatatt tggaagtgtc tcaaaagatg aatcaaatat tctaccaggg tttttctgat
     2341 gctgaaattg ttgcctttga aaaaacatta accggaattc tgaatcattt aaagcaatat
     2401 gaaaaggggc aatagatagc agtagcaact gcatatcatt aatataaatg acgaaaggaa
     2461 tgaatcccct ctatggatgg aaaatttatc aaagaaataa acaggcttgc gaacgccaaa
     2521 aacatcattg tcagttccgt cgacgggcag ggctatccga actccaaggc aatgttcaaa
     2581 gtcgcccatg acggactaca aaccttttac ctctccacca atacctcatc catgcgcgtc
     2641 caacaatttt taaaaaatcc caaggccagt ctgtattttt ttgacagaat caaagtccac
     2701 ggattgatgt tggtcggtga gatggaggta ttaaccgatg atgcaacgaa aagacggttc
     2761 tggaaaccct tctggaaaat atattacccc aaaggtgtaa ccgacccgga ttatgccatt
     2821 ttacgattca ccgcgaaaag cggcaactat taccggtccc ttcaaaaata ccgttttgag
     2881 attgatgaat aaaaatgtga gtgaaattct taatccatgc caaaataaaa aacactctca
     2941 atgttccagc ttgagagtgt ttttttatga gtcaaaggtt taatattgaa cttttaccgg
     3001 tttcccagtt cgcgccgatt ccataattgc caaaattacg agtgaagtat tggcagcatc
     3061 ctcaacagag actaaaaact tttcaccgga tgcaagcttg tctacaaaat ggcgaatgct
     3121 ctcgtaggca aaacccttgg ctttaccatg aacaaaatgg cgtaccagca catcggggcg
     3181 atcatttttt tcatccgtaa acctttcgat catctggctg ttacttaaat ccagattcat
     3241 catacccttt gtcccggtta tattaaactt gatatcattg acgcagggat atgtgttcgg
     3301 agtaatccag ccgttttcga gactggcaat cccgccatgg cggaactcca agatatattg
     3361 atatatatcg gtcgtatcta cacccagttt ctttaaaacc ccggagctgg acgcagcgta
     3421 caccgtttct acctcgtcat tcataaacca gcgcaccgtg tccacagcat ggctgcccaa
     3481 aaaccacaaa atcgaggact tggccgccca aggaagcata tcggtcgcta cccatttgat
     3541 gtcattcaac cgataatagg cgctaaccgg atcgcctaat tctccgtcgt ccaatgcctg
     3601 tttcgctaca gcaatcgggg gattccaccg gttatgcaag tcaaccatta atcgtacaat
     3661 atttgatttc accgcattca caatggcttc tacatcttcc ctggtagtgg ccaaaggttt
     3721 ttccaataaa atgtgtttgc cggcattggc acaatcgacc gccagttgcg catgggcaaa
     3781 atccggggtt acaatggcca ccgcgtcgag atcaacctct ttcaacatct ggcggtgatc
     3841 cgaataaacc tgtttgattc caaatttttg ggccagtttc tcagctctcg ccttgttcag
     3901 atcgcatatc gcgaccaggt caaccagggg atgctcctga tataagtatg cgtgagtttc
     3961 cccccatact cccgccccga tgatggcaaa ttttaatttc gacatttctg gttcctcctt
     4021 attctttaac cgctccaaaa gtcagacccc gaatcatata tcgttgcacc aacatggcga
     4081 agattaatac tggtaatgct atcaacacgc ccacagccgc catttccccc caaataactc
     4141 cggtaatcga taaaaagaag gttaccgtag tgggaagggt tctcgcatta acgcctgtta
     4201 aaaatagtgc aaaggcaaat tcattccagg agttaataat acagaaaata gccgttgcgg
     4261 ttaatcccgg caatgttaag ggcaacaata tttttgttag cgcctgccaa tgggagcagc
     4321 cgtccactaa tgcagattcc tcgatttgcg cgggaagttc ttcgaaaaac ccccgcatca
     4381 tccagactgt aaaagggata ttgaaagtca tatacgccaa aatcaaaatg aggtgcgtat
     4441 ccaaaagtcc ggtaaaccgg gctaaaataa aaaacggaat cacggtcgcc atgggcggca
     4501 acatccgcaa acctaaaatc caaaaagcaa catcttcttt tttcgaaaaa gaaaaccggg
     4561 cgaatccata tgcggccatg cttcctaata tcaaagaaat aacggtggta caaataacaa
     4621 caatcagact attacgcaca aaaagcataa tatggcgctg ttgaaacaag gcaagatagt
     4681 tttccagagt gggtgtgaat atccagacac tgggatcgta agtcaacacc ctggttttca
     4741 aactcaccag caacatccaa aagaagggga aaaccgctat aatacagacc agaacgagtg
     4801 ctagcttcca aagcaattgt ttgattttct tcatatttta gtttcaccct ctttgtggag
     4861 ctgccgaacc aaaaactgac tcaagacggt gataataatc aacaaaacaa ctccagtcgc
     4921 cgatgccctg cctatccatc ctgtttgggc aaaacccagg ttataaatgt gcaagcttaa
     4981 tagctcggta gcgctgcccg gaccgccttg agttaaaacg aatggcacat caaacagctt
     5041 taacgaatcc attgacctga ataatatcgc cagtaccaga agcggccgta atagaggcaa
     5101 tgtgacatat ttaaatgact gaaaaacacc ggccccatcc accaaggcgg cttcataggg
     5161 ttcttgcggc acagaagtca atccggcgta aataatcaag gccataaaag gcgtccattg
     5221 ccaaatgtct acgagtaggg ttgatgtata tgccagagcc ggtcccaacc aaacaacctt
     5281 ggtatgaaac agcaatccga taaaataatt gataacgcca tactccgcat tatacattag
     5341 cttccacatc tgccccacta cgcttggggt gatggtgatc ggaactagga ggcaagcaaa
     5401 taccagactt tttagcttaa atttatgatt taaaagcagt gcaacaataa atccgcaaat
     5461 ggtctcaaaa aaagtcgcta aaaacataaa aagcaaactg attcccaccg aagaccaaaa
     5521 ctcattatca cgcccgctaa aaagccggat ataattcttt aagcctacga cgtgaatatc
     5581 attccagaac tggcccagct cataattggt tacacttaca taataaagga aaatcatcgg
     5641 tacaatggtc aacaagacca aaatgataac cgaaggaaga atataggccc agtgcgggtt
     5701 tcttgcgaac catgatcttt tctgtgaatt tgacaagtcg ataaactgaa cctccctccc
     5761 aatctcaata tactgcccta aaagaatgcc gtattgatga ataatcggga ggaggagtcc
     5821 caaatcgctc ctccttccga tgtagtttat ttactcggac ataatatctt cgatttcaac
     5881 ctgggctttc ttgcacgcat cttcaggggt tatctccccg gttaaagcca gattcccgta
     5941 gtaacccgcc cgatccagga tcttgtcaag cttggatgtc ggcggaagcc aggcgatacc
     6001 ttttttgacc aacgccgaag cgcaagacaa cgcttgaagt tgaaccggat acgacggatc
     6061 cttggcaacc aattccttat ccgaataaac ggaagttctg gttggtacgc cgccgttttg
     6121 aacatattcc ttcgctttca gcttgcttgt catgtaggtg ataaaagccc atgcagcttc
     6181 tttattacgc gaattggatg caatcgccac attccagccg gctaacactc ccgcgtcacc
     6241 tgcgggccct ttgggcgggg cgacaaagcc tactttatcg taaatcgtgg actttttggg
     6301 atcggttatc catggcacca gcgcagtcgc atcaaaccac atggctgtct ttcctgccat
     6361 aaaagcggaa acggcctctt catgagtata cgaagccaca tccggcggag catttcgctc
     6421 gagtgccaca aaatacttca gcgattgaac ggtccggtga tccgtcatcg ttactttcca
     6481 ggtcttctga ttcacaaaac cgccgccaaa attatacatt acagtcatcc agccggaagc
     6541 gaaatggatc cccctctggg ctctcatggc gataccataa agcccaggct ccttgccatt
     6601 gaaaaacttg gccaattcca acatctcatc cattgttttg ggcggtctct tgccgtactt
     6661 ggcgaataaa tccttacgat aaccgacaaa acgggtttct cccgcagtcg gtatgccgta
     6721 gatttggcct tgatacgtgc caattccact gctgtatgcc ggaacaatat ccttataatc
     6781 aaaccagccg ggagtcgttt tactattgat aaatgacttt aagggggcta tgaccttttt
     6841 gggagcgtac tcggacatcc aaaatccgtc aaccataagc acgtcatata aatgactgtt
     6901 accggtataa tccatcaatt gcttgttttt gagatacgtt tcctccatta catcccattt
     6961 aaccttgata ccggttaact tttcaaattc gggtgtcatt ttttgaaatg cttcagtgga
     7021 tgggtggctg gcgaaagcaa cattaattgt tgtgccgtta tattttgcct ttattgcttg
     7081 agcccactga tccaaactgt cagtcttggc aaagacggca tttgacaaag ccagcatacc
     7141 tactaacaag acggcaaatc cctttttcat ttccatgtcc ctcctaatca agatatataa
     7201 ttagagccct ttcttaaacc ggcaaaacta ttgatccacc tcctttgcat ccgcagattc
     7261 agcggataat aaacttttat aacacacttt ttcaaagaaa tactgccggg catatgcggt
     7321 tgctccttgc agtatagcgt cttcaccgga gatcgagcgc ataatctcga aacctccccg
     7381 tgcgaatggt aaaacttctt taaaaaccaa ttggttgagt tccatccaaa atgttttgga
     7441 atcacacagt ttactttgga ttacaatggt atccgctccc atcaggttga gaacattcac
     7501 aatggcaacc gagatatttc tggcaaccgt ttttaagatg ccttgagcta aaagattttc
     7561 ttcgttatct tgagcaacca accattcaaa gatgttgttc tcatcaatat cgggagcctc
     7621 caagcttttg accttgcaga tatccgtcaa gatctcacat acagccgcgc ggctcaccct
     7681 ggtctccaaa caacccttcc ggccgcattt acatggtttg ccttccatat cgacaaccat
     7741 atggccgatt tcgacttcac tgccgcgtaa cccgtgatat aatttatggt ctgtgacaat
     7801 tccggcgcca accgccgatt caatccggac atataccaag ttcttactgt tgatagccct
     7861 tatatccagt tcaccgtatg cagccagtcc ggtggcattt tccgccaaaa cagggatatt
     7921 aatattcttg gttaattggg aaccgaccgg aaccgacttc catcccaaat ttaccgctga
     7981 aagaatgcaa ccgtccaggg aaataatgcc cggaagcgcc agcgtaatac aaacaatacg
     8041 gtttaattcc ttccccgaga gatcctttat catatttgtt aaaacaggta ctaattcatc
     8101 cggttttttt accaaaccgg atgacctttt cacttcattg ccaatcaagt cttcaactct
     8161 catatgtatt tggccgttat catccacatg ggctgcgcaa acccaccgtc cttccggccg
     8221 aattcgcagt ataatggggc gacgcccgcc gatcgactcg ccgcttccgg tttcttcaat
     8281 aattcccgct tctaaaagac gattaatgac ttgactaaca gtcgatcgac ttaaacctgt
     8341 ttttttgctg atttccgctc gcgaaatgga gcgcatctct cgtaccagat ttagcactaa
     8401 agttatattc tggcctttta cccaagcatt attggcacgc atcagccgca cgaaccagcc
     8461 ctccaaaagt ttgtttgttc tttgctcgaa caaagtagat aaagtttttc cctccatttt
     8521 actttctgtt cagaattttc tcagctagaa atggggggtg ctttcttaat aaaagtcact
     8581 atgtttgttc ttagttcgaa caatgttaat taattcttcg ttacaatttt atttcctgct
     8641 gatttcaaaa aaacttttat tccaaacatt ggataacggt ttttaattca atgcggttgt
     8701 ttaaagacgc atcacctttc cggtccaacc atttctttgc ctgatcaata aaaccagggc
     8761 atcttctttc tttgctgcgc ttgccaaaga gttcccggag ttatcccaaa ccgcactttt
     8821 gcctaccgga tgatacttac cggttggggc attataattt gccatcacca cagccattga
     8881 atactccctt gcatattgtt gcagctgttt ttttgactca gaatacccat tcatcat
//
LOCUS       DASVMB010000572         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_216327,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000572 DASVMB010000000
VERSION     DASVMB010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216327"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VF531_16060"
     CDS             <1..650
                     /locus_tag="VF531_16060"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX7715534.1"
                     /translation="ITAETRRFKHQEFAGITLNDYRTGDPRYQLLIRGTQAADKADPE
                     VQRIARRLRESAGSRQVVDLADAVYHYVIGKLTYRSNESLTGSVSVARALGEPSAAIC
                     EDYAILTVALLRALGIPASYYSGDTYGKSFGHAWAVFYTPDYRPVPLDPTWGDTSGAP
                     DLYFLASSNLNLVRSFDYDSELMPGSTSIRMDTTSDPSLTTRLGEVNLTLTRLNE"
     gene            complement(654..1175)
                     /locus_tag="VF531_16065"
     CDS             complement(654..1175)
                     /locus_tag="VF531_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7715535.1"
                     /translation="MKIEMNFDSSWMDDLHELQKRAAELGGNENPSNAALELAMDLLK
                     KWGVDSIPWNNNFESLKSLWAKGNLYGGPNKNIDISERKQDVLVQMTIPGIKDQNDLS
                     VSFANQTLYINAKSNAYEKEGGSFSREIRLPAAVTTEGATAAYRNHLLTVTLPKTAPQ
                     KGEKIPVSFLPAE"
     gene            complement(1165..1410)
                     /locus_tag="VF531_16070"
     CDS             complement(1165..1410)
                     /locus_tag="VF531_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715536.1"
                     /translation="MDLQIKIADLKVSEITTTSGIFSGENYHAHWSAISKSNTGLNLG
                     DSSLSTACINMVYDPDFIGLIKTSKKGFQNRSKVDED"
     gene            complement(1421..1711)
                     /locus_tag="VF531_16075"
     CDS             complement(1421..1711)
                     /locus_tag="VF531_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715537.1"
                     /translation="MKECSLELTIHSITIGSIENASSFNIGRNMLRDFKSMSKSNAGI
                     GTISGNNNSFPSSTNYVQDPDGIDMYWDGEDGTGEDPAARKMPFPLFKGERK"
BASE COUNT          435 a          468 c          451 g          537 t
ORIGIN      
        1 agatcacggc ggagacccgt agattcaaac atcaggagtt tgccgggatt acactgaatg
       61 attaccgtac cggtgatcca aggtatcagt tactgatccg cggcacccag gcggcggata
      121 aggctgaccc ggaggtgcaa aggattgcga ggcgcctccg ggaaagtgcc gggagtcggc
      181 aggtcgttga tctggctgat gcagtctatc attatgttat agggaaattg acctatcgga
      241 gtaatgaatc cttaaccgga agcgtcagcg tggccagggc gcttggagaa ccctccgcgg
      301 ctatctgtga ggattatgcc atcttaacag tcgctctgtt acgtgccttg ggaataccgg
      361 cttcctatta ttcgggggat acgtatggca aatccttcgg ccatgcctgg gcggtttttt
      421 atactccgga ttatcgcccg gtcccgctgg atccaacgtg gggcgatacc agcggcgccc
      481 ctgacctcta ttttttagcc agcagtaatc ttaatctggt ccggagtttc gattacgatt
      541 ccgaactcat gcccggctct accagtatca ggatggatac cacttccgac ccaagcctca
      601 ccacccggtt aggagaggtg aatctgaccc tcacgcgctt gaatgagtga ttcttactcc
      661 gccggtaaaa agctcacagg aatcttttcg cctttttgcg gtgctgtttt aggaagggtg
      721 acggttagaa gatgattacg gtaggcggct gtcgcacctt cggttgtaac tgcagccgga
      781 agccggattt cccggctgaa ggatccccct tccttttcat acgcattact tttcgcatta
      841 atataaagag tctgattggc aaaacttacg gacaggtcgt tttggtcctt gattcccggt
      901 atggtcatct gaacgagtac atcctgtttt cgctccgaga tatcgatgtt tttattaggt
      961 ccgccgtata gatttccctt cgcccatagt gactttagcg actcaaagtt gttattccag
     1021 ggtatactgt ctactcccca ttttttgagt aaatccattg ccagctccag agcggcattt
     1081 gaagggtttt cattccctcc aagctcggca gcacgtttct gaagttcatg taaatcatcc
     1141 atccatgatg aatcaaaatt catctcaatc ttcatctacc tttgaacgat tttgaaaccc
     1201 ctttttggat gtcttaatca aaccgataaa gtcgggatca taaaccatgt tgatacaagc
     1261 agtgctaaga ctggagtctc ccaaatttaa accggtgttg gatttgctta tcgccgacca
     1321 gtgggcgtga tagttttctc cgctaaaaat acctgaggta gtcgttattt cagaaacttt
     1381 caagtccgcg atcttgatct gcagatccat gtgaccaacg tcacttcctt tctcccttaa
     1441 aaagcggaaa cggcattttc cgggccgccg gatcttcgcc ggttccgtcc tcaccatccc
     1501 agtacatatc gatgccatcg gggtcttgca cataattggt gcttgaaggg aaggaattgt
     1561 tattccccga aattgtcccg attccggcat tggatttgct catactttta aaatcgcgta
     1621 acatattgcg gccgatattg aacgaggagg cattctcaat acttccaatg gtgatggagt
     1681 gaatcgtcaa ttccagactg cattctttca ttggcaatct cccgtgaatc ctctacttat
     1741 ttcgggcggg gccaccggtt cactcaccgg agcaactacc ggcgcgtatg atgtagtata
     1801 ctttatgact tttgcatccg aggtgtgcca tggcggtttt tataaacttc ggtttgataa
     1861 tcattaacaa cttggccagt aatgccggac t
//
LOCUS       DASVMB010000573         8528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_293304,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000573 DASVMB010000000
VERSION     DASVMB010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293304"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VF531_16080"
     CDS             complement(<1..1367)
                     /locus_tag="VF531_16080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715538.1"
                     /translation="MNYFKLAVKNIRHDLKNYLSYFISTTFAVMVFNIFSTVFFNPQF
                     KALSQVKVKLDLLFRASAVVVAVFAAAFIWYANTFFIKQRKKEMATYSLLGLGKRQVA
                     AMLFFENLITGLLSLICGIVLGTLFAKLFAMMLINLMHELVVVRFIVVWQAIGATALV
                     FIILFLVNSVHAYSLIYRFKLIDLFTAAREGEPAPKASVISSVISLITLFIGYAIAIH
                     QANHLSVYTKNYLLPVLSLIIAGTYLLFNNLIIIVIRGLKRQKDYYYQGINLISTSNL
                     VYRIKGNAHSLAIIALLCALSITAIGTTYAYYKSNDSYIGSTSPYSFMHLGEDALLNR
                     RVTQIIQHNGAIQLRAIDDFQLIEGFSSTPRYPGPYGKKMQQVPTSFISLSQYNRAMG
                     HQRRPLIRLANDNQCYFLELNAGNGQGHSLQNQPVEVSLAGKSRMLTIIGSTTRLLIN
                     SNLART"
     gene            complement(1354..2133)
                     /locus_tag="VF531_16085"
     CDS             complement(1354..2133)
                     /locus_tag="VF531_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7715539.1"
                     /translation="MNLLTVKNLKKIYGSKWGGSICHALNGIDFEVAAGEFVGVMGPS
                     GAGKTTLLNIIATIDTPTSGSVRIDGTEITSLNETELSDFRRTFLGFIFQDFNLLDTL
                     TVQENIILPLSLAKVKARACATEIEARLEQVSVILGIRDILAKYPYEISGGQKQRTAA
                     ARAIITKPKLILADEPTGALDSKSSLELLQALSEINTENQATILMVTHDAFAASFCRR
                     IIFIKDGLLFAELLRGDGRKEFFDKILSVLAALGGDNRELF"
     gene            2421..4316
                     /locus_tag="VF531_16090"
     CDS             2421..4316
                     /locus_tag="VF531_16090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7715540.1"
                     /translation="MRISGGFNWLLVIGVIFSSLFCRNMVHAETSDQSVQAFARMTDG
                     YMKQVLNEYKVAGVAVVIVKDGAVFFEKGYGYADLAQKIPVNPRTTAFQIASVSKLFT
                     ATAAMQLVEQGKLSLDEDINHYLTDFKIKNPFPKPVTLRTLLTHTSGLDDRTPVYIRT
                     PGTRFFDSIEPLESYIKRDLPPVVKEPGSYCQYNVYGMALVGYLVQKVSGVPFDQYVT
                     DHILKPLAMKHSSYELTPAILKSMAKPYRFVKGEYLEGAYTLISDHPSGSICATASDM
                     AKFILMNLNHGEYPGGRILNGDTLREMHRHQYPADERLTGFGLGFYETIRNGYRTIEH
                     GGYLPSFSSKLTMLPERNIGMFIAINTDSKDSSRVCNDFVDKFYGFFTTRVRSEKALA
                     ALQAGVPLDMDPQVINGNYTYDAYGRTDLTKLKSLLVTCNIQCDPSGNLTFTAENLKW
                     KFGYVGDGYFYNMQRDMYCRITEANGKMVLSVLGGDYEKVSDLEAALFKAAVAGIPVF
                     LIAIVMLLISLIKNRKCRDKQAMLLKGSLLGTGLLIVTYFVLNGVMGVKSMQCDTYAV
                     IHGIIPFIKVTCYLTLGFGAASIVSVTNSWINKSISLLSRIFYSIIVIWAVVNLVFMY
                     LMNGFRL"
     gene            4355..4618
                     /locus_tag="VF531_16095"
     CDS             4355..4618
                     /locus_tag="VF531_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715541.1"
                     /translation="MFNCKSFFIKAILLSFIISGLITTTPGHPVQAAGINPDRYLNVK
                     TKVTAFIKKEMKNRKVQGLSIALVDDQEVVWTEGFSWRVRPIA"
     gene            4576..4905
                     /locus_tag="VF531_16100"
     CDS             4576..4905
                     /locus_tag="VF531_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715542.1"
                     /translation="MDRRFQLAGETYRLTPRKSGRCSLEKMLFGIFPLKAPDLMDLRL
                     QNISGKDLMIVPDDHGPVILGEKVEPSPLGPDWRKRAGTYEVVNLGSDLPLFEKRTRL
                     TIESGMR"
     gene            complement(5161..6201)
                     /locus_tag="VF531_16105"
     CDS             complement(5161..6201)
                     /locus_tag="VF531_16105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715543.1"
                     /translation="MKLRDYLRDRRTGIIFYSVIMAFIIAVIYLGPAHSVVSEDLLYI
                     LIVSTFLGVGYLTVDYTVNRNHCRRLNSLINSPNQNWFYNLPEPRNTEERIYQDLLKR
                     LNNDFRNDLDRFTEQNQADLEFVTTWVHQIKTPIAAARLLLENSLRRNRPVVEDLHSL
                     EEELSKIEGDVQKALYYSRSNDFAKDYFIASTHLETLVKECVKKQATVFIHKKIRVII
                     EPITYEVATDIKWLGFILDQLLTNALKYTGPGGTITIRADAVENEIRLEVADTGIGIK
                     PEDLPRVFQPSFTGDNGRKNLTATGMGLYLSQKLARKLGHYLTIASRNHEGTVVTIHF
                     PKWASYFDTESK"
     gene            complement(6198..6878)
                     /locus_tag="VF531_16110"
     CDS             complement(6198..6878)
                     /locus_tag="VF531_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003565958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715544.1"
                     /translation="MLPLKIFIVEDDSKIAGVILQNLNKWGYEGVAAQDFNRVCDEFL
                     QCKPALVLLDINLPAYDGFYWCRRIRQFSKVPIIFISARNANMDIVMAVNMGGDDYLT
                     KPFSIDVLIAKVSALLRRTYSYNDSGLDLIEHRQAVLNLKDDTLSFAGQKLELTKNEF
                     RILYVLMKEHGSLVSRERMMRHLWEDENFIDDNTLTVNINRLRRKLDEIGLVDFIQTK
                     KNRGYIIP"
     gene            complement(7112..>8528)
                     /locus_tag="VF531_16115"
     CDS             complement(7112..>8528)
                     /locus_tag="VF531_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7715545.1"
                     /translation="IVVLDNRSHEILAMVGSGDFNDRLHDGQVNATLARRSPGSALKP
                     FIYAQSMETGLVSPAHYLEDVPTDFSGYAPENYDRTFCGVVSARAALERSLNLPAIGL
                     EQQLENGSTNLGLYTLLRKAGVTMRSRDHYGLSIAIGGCEVSLLDVTALYSAMANGGE
                     YTKPRWLLSGREAAPIKLFDAGTAYIITDILTGLRRPDLPTCWEFTSLPQIAWKTGTS
                     YGHRDAWSIGYNPRYTVGVWLGNFSGEGAPGLIGAEAAAPILFELMNSICRGQQIRWF
                     KAPDSVDWRQVCSISGQPAGPYCPTAISEIYLPDRSPDKECQFHQAIQIDDLTGLRLP
                     PDYAGERKSHTKVYLQWPARVGSWMEASGSPPERIPQLLPGWQGLLPGASPVIRSPSQ
                     DCQYRLREGIDRTYQKICLEASAGSDVHRLYWFIDGELLGTVKPGERLFYLPVPGRHR
                     LVCQDDFGRSTEMPLVIERAE"
BASE COUNT         2153 a         1964 c         2249 g         2162 t
ORIGIN      
        1 gtccgggcaa ggttggaatt gatcaataac cgggtggttg atccgatgat ggttagcatt
       61 ctgcttttac cggccaggct aacctcgacc ggctggtttt gtagactgtg accctgaccg
      121 ttgccggcgt tgagctccaa aaaatagcat tgattatcgt ttgccaatct gatgagcggg
      181 cggcgttgat gacccatggc ccggttatat tgactcaagg atatgaatga agtcggtact
      241 tgctgcatct ttttaccgta aggtcccgga taacgggggg tgctggagaa gccctcaatt
      301 agttgaaaat catcaatggc tctcaactgt attgcaccat tatgttggat gatttgtgtc
      361 acccgacggt tcagcagggc atcctcaccc aggtgcatga aggaatatgg cgaggtagag
      421 ccgatgtatg aatcattgga tttataataa gcatatgtgg ttccgatggc tgtgatcgac
      481 aacgcgcaga gcagcgcgat gattgccaat gaatgggcat tgcctttgat ccggtaaacc
      541 agattggagg tgctaatcag gttgatccct tgatagtaat agtctttctg ccgttttaga
      601 ccgcggatta cgatgattat caagttatta aagagcagat atgtcccggc gatgatcagg
      661 ctcagtaccg gaagcaaata gttttttgtg tagaccgaaa gatggtttgc ctggtggatg
      721 gcaatggcgt acccgatgaa aagcgtgatt aaactgatta cactacttat gactgaagcc
      781 ttcggggcgg gttccccttc tcgggccgcg gtaaaaagat cgatcagctt aaaacggtaa
      841 atcaggctat aggcgtggac cgagttgact aaaaaaagga taataaagac taacgccgtg
      901 gcgccgatgg cctgccagac aacgatgaac cggaccacaa ccaactcgtg catcagattg
      961 atgagcatca ttgcgaataa ctttgcgaac agggtcccca ggacgatccc gcagatcaat
     1021 gagagcaggc cggtaatcag attttcaaaa aagagcattg cggcaacctg tcttttgccg
     1081 agacccaaaa gggaataggt ggccatctcc tttttgcgtt gcttgataaa aaaggtattg
     1141 gcgtaccaga tgaaggcggc tgcgaaaacg gccaccacaa cggctgaggc ccgaaacaac
     1201 agatccagtt tcacctttac ctgacttaag gccttaaact gcggattaaa gaaaaccgtg
     1261 ctgaagatgt taaaaaccat taccgcaaag gtggtgctga taaagtagct taagtaattc
     1321 ttgaggtcgt gccggatgtt tttaaccgcc agtttaaaat agttcacggt tgtcaccccc
     1381 caaagcggcc aggaccgaca gtattttatc gaagaattcc ttgcggccgt caccccggag
     1441 aagctccgca aagagtagcc cgtccttgat aaaaatgatc cgccggcaga agctggcggc
     1501 aaaagcgtca tgggtgacca tcaagatggt tgcctgattt tctgtattga tttcactcaa
     1561 agcctggaga agctccaggg aagacttgga gtccagggcg ccggtaggct catcggctag
     1621 gatcagcttg ggtttggtga taatagcccg cgccgccgca gttcgttgtt tttggccacc
     1681 cgaaatctca tagggatact tcgctaggat atcccggatg cccaaaataa cagagacctg
     1741 ctccagtctg gcttcgattt cggtcgcgca cgcgcgcgct ttaaccttgg caagactcag
     1801 gggcaggatg atgttttcct ggaccgtcaa ggtatccagc aagttgaagt cttgaaagat
     1861 aaagcctagg aaagtgcggc ggaaatcgga aagctcggtt tcgttgagac tggtgatctc
     1921 agttccgtca atccgaacac tgcccgaagt cggggtgtcg atggtggcga tgatgttgag
     1981 cagcgtggtt ttacccgccc cggacgggcc cataactccg acaaactctc ccgctgccac
     2041 ctcgaaatcg atcccgttca aagcgtggca gatactgccg ccccatttgg aaccataaat
     2101 ctttttgaga ttttttaccg ttaatagatt catttcccgg attgcctcct atgttcattc
     2161 cgtaaatcgg cttggtcatc cgtatagcta aattatatgg gaggctcgct ttgaagaaaa
     2221 ttgattcaac ttacatttta tcatccaaac ttacgttttt gtcattttag gcgaatatta
     2281 caaaactgta agtttcatag tgaaaatgta agccaaaaaa atggcggggg aagggcttct
     2341 ttgttacgat caaaaagacc gctattcggt cgtaggcgtt ggtcatccga acaacaaatc
     2401 atgaacaggg aggacccaag atgagaatat ccggtggatt taactggttg ttggtaatcg
     2461 gtgttatctt tagttccttg ttttgtcgga acatggttca tgcggaaaca tccgaccaat
     2521 cggttcaggc ttttgcgaga atgacggacg gttatatgaa gcaagtattg aatgagtata
     2581 aggtggcggg tgtagccgtt gttatcgtga aagacggcgc ggtgtttttt gaaaagggtt
     2641 atggttatgc ggatttggct caaaaaatcc cggtgaaccc aagaactacg gcatttcaaa
     2701 tcgcatccgt atccaagctg tttacggcaa ccgctgccat gcaactggtc gagcagggca
     2761 aactaagctt agatgaggat attaaccact atttgaccga ctttaagatc aaaaatccgt
     2821 ttccaaaacc agttaccctg cggacattat tgacccatac ctcgggcttg gatgaccgga
     2881 ccccggtcta tattcgaacc cccggtacca ggttctttga ttccatcgag ccgcttgaga
     2941 gctacataaa acgggatttg cctccggtcg tcaaagaacc gggcagttat tgccagtata
     3001 acgtctatgg tatggcgctg gtgggttatt tggttcaaaa agtatccggc gttccctttg
     3061 accagtatgt tacggaccat attttgaagc ctctggccat gaaacactca tcatacgaat
     3121 tgacccccgc gatactgaag agtatggcta aaccataccg gttcgtcaag ggagagtatc
     3181 ttgagggggc ctatacatta atcagcgatc atcccagcgg gtcaatttgc gcaaccgctt
     3241 cggatatggc taaattcatt ctgatgaatc tcaatcacgg agagtacccg gggggcagga
     3301 tattaaatgg ggacaccctc agggagatgc atcgtcatca atatccggct gatgaaaggt
     3361 taacaggatt cgggctgggg ttttatgaaa caatccgaaa cggctaccga accattgaac
     3421 acggcggata tttaccctcg ttctcttcca agttgacgat gcttccggag cggaatatcg
     3481 ggatgttcat cgccatcaat accgattcca aagatagcag cagggtctgt aacgattttg
     3541 tcgacaagtt ctatggtttt tttaccacca gagtccgaag tgaaaaggcc cttgccgcat
     3601 tgcaggccgg tgtgccgctg gatatggatc cgcaagtcat taacggcaat tatacctatg
     3661 acgcgtacgg tcgtaccgat ctgaccaagc ttaaatcatt gctggtaacg tgcaacattc
     3721 aatgcgatcc gtccgggaac cttacgttca ccgccgagaa tctcaaatgg aagttcggct
     3781 atgtcggcga cggttatttc tataacatgc aaagggatat gtattgccgg attaccgaag
     3841 cgaatgggaa aatggtctta tcggttctgg gcggcgatta tgaaaaagtg tctgacctgg
     3901 aggcggcttt attcaaggct gcagttgccg ggataccagt ctttctgatt gccattgtga
     3961 tgcttttgat atctttaatc aaaaacagaa aatgccggga taagcaggcg atgctgctca
     4021 aaggttcatt actgggaacg ggtctgctga tcgtaaccta ttttgtattg aacggggtta
     4081 tgggcgtaaa gagtatgcaa tgcgatacct acgccgtgat ccatggaatc ataccgttca
     4141 tcaaagtaac ctgctattta acccttgggt ttggggctgc ttcgatagtt agtgttacga
     4201 actcctggat caacaagagt atctcactcc taagccggat tttttattcc atcatcgtaa
     4261 tttgggctgt tgttaatctg gtattcatgt acttgatgaa cgggttcaga ttgtaggggg
     4321 gattctatat cacagaagaa tgggggcaaa tcctatgttc aactgtaagt cctttttcat
     4381 taaagccatc ttattatcgt ttatcatttc agggttgatt acgacaactc ccggccatcc
     4441 ggttcaggcg gccggcatca atccggaccg gtatttgaac gtaaaaacaa aagtgacggc
     4501 ctttatcaaa aaagagatga aaaaccggaa agtccagggg ctaagtattg ccctggttga
     4561 cgaccaggag gtggtatgga ccgaaggttt cagctggcgg gtgagaccta tcgcttaacc
     4621 ccccggaaaa gtggccggtg ttcactggaa aagatgttgt tcgggatatt tccgttaaag
     4681 gctccggatt tgatggattt gcggcttcag aacatctccg gcaaagactt gatgattgtt
     4741 cccgacgatc acgggccggt gatattgggt gaaaaggttg aacccagccc gcttggtccg
     4801 gattggcgca aacgggccgg aacgtatgaa gtggtcaatc tcggttcgga tctgccgctt
     4861 tttgaaaagc gaacgaggtt aacgattgaa tcgggtatgc ggtaataaag tattcaggtg
     4921 tatttaatcc gaattacttc tttgcgcttc agcccctcgc cgcagatcgg gctgtggtgc
     4981 tgggctctgg cggggttgcg gtgaagcagt gaagatagtg acaatagcgg atcctgccac
     5041 cggcagggcg gaggaacggt tatggttttc cgggtatgag tttcgtaaga taaattagga
     5101 acaacggggc tgactcaaaa gcaaacaaat gataaatttg tcttttgaga cagccccgat
     5161 ttatttggat tctgtatcaa aataactggc ccacttggga aaatggatgg tcacaacggt
     5221 tccctcgtga ttccgggaag caatcgtgag ataatgcccg agtttacggg cgagtttttg
     5281 gctcagataa agtcccattc cggtggcggt aaggtttttt ctgccgttat ccccggtaaa
     5341 gctgggctga aagacccggg gcaaatcttc cggtttgatg ccaatcccgg tatccgccac
     5401 ctccaggcgg atttcgtttt ccaccgcatc agccctgatg gtaatggtcc cgccgggtcc
     5461 ggtgtatttg agggcgttag tcaatagttg atcaagaatg aagcccagcc acttgatatc
     5521 ggtagccact tcataggtga tgggttcgat gataaccctg atctttttat ggataaaaac
     5581 cgtcgcctgt tttttgacgc actccttaac aagggtttcc aaatgagtcg acgcgatgaa
     5641 ataatccttg gcaaagtcat tgcttctgga gtaatatagg gctttttgca catccccctc
     5701 gattttggaa agctcctctt caaggctgtg caaatcttcc actaccggtc ggttgcgccg
     5761 caggctgttc tccagcagca gccgggcggc ggcaataggc gtcttgatct gatggaccca
     5821 ggtggtaacg aactctaaat cggcttggtt ttgctctgtg aaccgatcaa ggtcgttacg
     5881 gaaatcatta ttcaatcgct tcaaaagatc ctgataaatc cgttcctccg tattccgggg
     5941 ttcgggaaga ttatagaacc agttctggtt cggtgagttg atgagactat tgagtctgcg
     6001 acaatgattc ctgtttaccg tgtaatcaac ggttaaatag ccaacaccca ggaatgtcga
     6061 cacgatgagg atgtacagaa gatcttcgct cactacggaa tgggctggcc ccagatagat
     6121 aacggcaatg atgaaggcca tgattactga ataaaaaatg atgccggtcc gccggtcccg
     6181 gagataatcg cgaagcttca tgggatgatg tatcctcggt ttttttttgt ttgaataaaa
     6241 tccaccaaac cgatctcgtc cagtttgcgg cggagccggt tgatattcac ggtgagcgta
     6301 ttatcgtcga tgaaattctc atcctcccat aaatgacgca tcatccgttc tctggagacg
     6361 agcgaaccat gttccttcat gagcacatat agaatccgaa actcattctt ggtgagttcc
     6421 aatttctgtc cggcgaagct taaggtatca tccttgaggt taaggacggc ctggcggtgt
     6481 tcgatcagat ccaggccgct gtcgttatat gagtatgtcc ggcgcaatag ggcactgacc
     6541 ttggcaatta aaacgtctat cgagaacggt ttggtcagat aatcgtcacc gcccatattc
     6601 accgccatga cgatatccat gttggcattc cgggcggaga tgaaaatgat cggcaccttg
     6661 gagaattgcc gtatccgccg gcaccaatag aaaccgtcat aagcggggag attgatatcc
     6721 agcaaaacca aagcgggctt acattggagg aattcgtcgc agacccggtt aaagtcttgg
     6781 gcggcaacac cttcatatcc ccatttattc aggttttgca ggatgacccc tgcgattttc
     6841 gaatcatcct cgacgatgaa gatcttgagt ggtaacattc aaataaccct cgcaaatcga
     6901 atcatctaat tggtattgat tataacaaat taaaggtcag gaaggcttat tgatttgtat
     6961 acatttccaa ctaaattttt gggctgttat ttcctattcc ttgacgattt tgtgggtaat
     7021 ccaaaatgaa ccacgaaaca cacgaaaatc acgaaaacgt tgctgttctt ttttcgtgtt
     7081 cttttgtgca attcgtggtt aaaagttagg tctattccgc gcgctcgatc accaggggca
     7141 tctcggtgct ccggccgaaa tcatcctggc aaactaaccg gtggcgtccg gggaccggca
     7201 ggtaaaagag gcgttctccc ggtttcaccg tacccaggag ttctccatcg ataaaccagt
     7261 aaagacggtg aacgtcacta ccggcggagg cttccaggca gatcttttgg taagtgcggt
     7321 caataccttc tcttaaacga tactggcagt cttgggaggg agaacggatt accggcgacg
     7381 cacccgggag taagccttgc catccgggaa ggagctgcgg gattcgttcc ggtggactgc
     7441 cgcttgcctc catccaggat ccgaccctgg ccggccactg cagatacacc ttggtatggc
     7501 ttttacgttc accggcataa tccgggggca gccgcaaacc cgtcaagtca tcgatctgga
     7561 tggcctggtg aaattgacat tctttgtcgg gagagcggtc gggcaggtat atttcggaaa
     7621 ttgcggtggg gcaatatggc ccggccggtt ggccgctgat ggaacaaacc tgacgccaat
     7681 cgaccgaatc cggtgcctta aaccagcgaa tctgctggcc ccggcagatg ctgttcatca
     7741 gttcaaataa tatgggggcc gccgcttcgg cgccgatcag gccgggggct ccctcgccgg
     7801 agaagttgcc caaccagact cccaccgtgt aacgggggtt atagccgata ctccaggcat
     7861 cgcggtgacc gtaagaagtt ccggttttcc aggcgatctg cggcaaggag gtaaactccc
     7921 aacaggtggg gaggtcgggg cggcgtaagc cggttaagat gtcggtgatg atgtatgcgg
     7981 taccggcatc aaaaagtttg atcggagcgg cttcacgtcc ggaaagcagc cagcgcggct
     8041 tggtgtactc gccgccgttt gccattgcgg agtaaagagc tgtgacatcc agcaggctaa
     8101 cctcacaacc gccgatggcg atggagagcc cgtaatgatc cctggaccgc atcgtcacac
     8161 ctgctttacg gagaagcgtg taaaggccta gattggttga cccgttctcc agctgttgtt
     8221 ccaggccgat ggcaggaagg tttagcgaac gttccaaggc cgcgcgggcg gagacgacgc
     8281 cgcaaaatgt ccggtcataa ttctccgggg cataacctga gaagtccgtg ggtacatcct
     8341 caagataatg ggcgggtgag actagtcccg tctccatgga ctgggcatag atgaaaggtt
     8401 ttagagcgga accgggtgaa cgccgggcca aagtcgcgtt cacctggccg tcatgaagcc
     8461 ggtcgttaaa atctcccgaa ccaaccatgg ccaggatctc atggctacga ttgtcaagaa
     8521 ccacaatg
//
LOCUS       DASVMB010000574         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_146366,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000574 DASVMB010000000
VERSION     DASVMB010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146366"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF531_16120"
     CDS             complement(<1..180)
                     /locus_tag="VF531_16120"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEX7715546.1"
                     /translation="MPDQSIHPLAGRKARPEDLYSKEQITDWYNHPQGELEPIKNGTS
                     GHRGHTGRGFSEAHVA"
     gene            454..1212
                     /locus_tag="VF531_16125"
     CDS             454..1212
                     /locus_tag="VF531_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7715547.1"
                     /translation="MMHLLSLLDLSTGQWVVSGLCVMLLGFSKTGIGGVSTLVIPVMA
                     AIFGGKSSAAIILPLLISGDLIAVRHYRTNANWPYIFKVLPWSFGGLALGVLVGNLVS
                     DLQFKMIIGVSVLFCLGIMIWMERHGDTATMPDKWWFGAIMGLAGGFTTMIGNAAGPV
                     MTVYLLAMRLPKYDFIGTGAWFFMIMNLTKLPLQIIFWKAITPATLAFDAVMFPAILI
                     GTFIGIRVVRKIPEKPFRVTVTILTAAAAVKLFF"
     gene            complement(1218..2537)
                     /gene="creD"
                     /locus_tag="VF531_16130"
     CDS             complement(1218..2537)
                     /gene="creD"
                     /locus_tag="VF531_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEX7715548.1"
                     /translation="MEVKGFNRNSIIVRLLMIGILILVLMIPTVMINGLIGERSIRKA
                     EAVAEITSKWAQAQTVGGPVITIPYERSFKDDQGKVRTEMDYAQFLPEELGIEGKIRP
                     EIRYRGIYKAAVYNLKLKLAGRFSFAKLERLNLNPERIHWNEAFMAVQITDMRGIKDS
                     VGLIWGGRQFRFEPGIFEGGIFTSGISLRIPLNTISAQEGSAFSINLNLNGSEELSLQ
                     PLGQTTTVQLSSTWNSPSFTGAFLPETRRVDKNGFNARWKVLDLNRNYPQQWVGDAYN
                     DNLKGSEFGVKLFSPVDEYTQVNRAVKYIFMFVGLTFLVFFLVEVFNKRKIHPVQYLL
                     IGLSLCIFYVLLLSLSEHINFQLAYLIAGISIIGLIGAYVRSALGGKWVTLLTAGLLV
                     ALYGYLYILLQNQDYALLMGSIGVFIIMAIVMYISRNIDWYKIEMKD"
     gene            complement(2588..3172)
                     /locus_tag="VF531_16135"
     CDS             complement(2588..3172)
                     /locus_tag="VF531_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715549.1"
                     /translation="MQTWYRKAWRIISQPYAFFEETINCGNTKAATITFVGALLLVNL
                     GWLAVTRESSPAFWVCGFLLPLLSYPAAVFIIYLICRILVRETKLRSFFAVWGFSFLP
                     TGLFFLLNIVTHILITIPGFGRFPSSPVFMLIFWTLIILIFLWKALFLAITLRLAGNL
                     NFRQMIPAFILLGLIAGGYWWVTSGLGWLKIPFI"
     gene            complement(3190..3495)
                     /locus_tag="VF531_16140"
     CDS             complement(3190..3495)
                     /locus_tag="VF531_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715550.1"
                     /translation="MNEQGLQRLIEKTELFRRKDREAAAQWHNRIVSRMIDTAHRETE
                     RLEYHLSELPLSVVIYQWVIVRLKSRPWRLLLPASLLLSALFHGVLARVNLIQLLVR"
     gene            complement(3492..4055)
                     /locus_tag="VF531_16145"
     CDS             complement(3492..4055)
                     /locus_tag="VF531_16145"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7715551.1"
                     /translation="MKNWPEIDDLTLVRQIQHGDEAAIEEFYCRFAPGLHSFIHCRVR
                     EPDDIEDILAETMTGAVTAIMRFKGQSRVFTWLCQIAKFKIADYYRRNTDRNMLPLDE
                     TLAATCCTETDSETNLLIWEALLKLSLEYRQVLEDKYFTGFSTREIAVRLGRSEKAVE
                     SILVRARRAFAEEYKRLDPETEVLRDE"
BASE COUNT         1061 a         1125 c          937 g         1026 t
ORIGIN      
        1 ggccacatga gcttcggaaa atccccggcc ggtatggccc cggtgaccgg aagttccgtt
       61 tttgatcggt tccaattcac cctgagggtg attgtaccaa tcggtgatct gttccttaga
      121 ataaagatcc tcgggccggg ctttccttcc cgccagagga tgaatcgatt gatcgggcat
      181 cagcgcgcct cctaatgtat cctaaccctg aggatggatt atgaccaact tcaagatcac
      241 tgatctatta tttccgtaac ggacagagaa attattttct cgatccataa ccttttccct
      301 gccccatagg taatttttta tcaagatctc ataaagtgcg atcatattcc cctataacac
      361 catgctttat tactctggat caaagaggag tttgaactgt tttgttaaat tctcatcgta
      421 gactttgtta caacatgaag ggctgtgctt aagatgatgc acctcttgtc actcctcgac
      481 ctctccaccg ggcaatgggt tgtatccgga ctctgcgtga tgctgctcgg tttctccaaa
      541 accggtatcg gcggggtgag caccctggtc ataccggtaa tggccgcaat cttcggaggc
      601 aagtcctcgg ctgccattat cctgccgtta ctgatctccg gagatctcat cgcagtcagg
      661 cactatcgca ccaatgccaa ctggccgtat atcttcaagg ttttgccctg gagtttcgga
      721 gggctggctc tgggtgttct ggtgggcaac ctcgtaagcg atcttcagtt taaaatgatc
      781 atcggcgttt cggtcctttt ctgcctggga atcatgatct ggatggaacg gcacggcgat
      841 acggcgacga tgcccgacaa gtggtggttt ggggccatca tgggactggc cggggggttc
      901 accaccatga tcgggaatgc cgccggaccg gtaatgaccg tttaccttct ggcaatgcgc
      961 ctgccgaagt acgatttcat cggcacgggc gcctggtttt tcatgatcat gaatctaacc
     1021 aaactaccgc tgcaaatcat tttttggaaa gccatcaccc ctgccacatt agcttttgac
     1081 gcggtgatgt ttccggcgat cctgatcgga accttcatcg gaatccgggt ggtgcgaaag
     1141 atccccgaaa aaccgttccg ggtgacggta actatcttga ccgcagccgc tgccgtgaaa
     1201 ctgttcttct aatatcatta atctttcatc tcaatcttgt accagtcgat attccgggag
     1261 atatacatca ctatcgccat gatgatgaag actccaatac tccccattaa taaggcataa
     1321 tcttgatttt ggagcaaaat atagaggtat ccgtaaaggg ccaccagtag gccagcagtc
     1381 aaaagtgtca cccattttcc acccaaggcg ctccggacat aggcgccgat taagccgata
     1441 atactgatac cggcgatcag atatgccagt tggaagttaa tatgctcaga cagggaaagc
     1501 agcaaaacat aaaaaatgca caatgaaagc cctatcagca ggtactgcac cggatggatc
     1561 ttacgtttat taaatacctc caccaagaag aaaacaagaa acgtcaagcc cacaaacata
     1621 aagatgtatt tgaccgcccg gttgacttgg gtgtattcat ccaccggcga aaacagtttc
     1681 accccgaact ccgaaccttt aagattatcg ttataggcgt cacccaccca ttgctgagga
     1741 taattccggt tcaagtccaa caccttccaa cgggcattga acccattctt atcgacccgg
     1801 cgggtttccg gcagaaacgc cccggtaaaa ctgggactat tccatgtcga gctaagttgg
     1861 acagtggttg tttgccccaa cggctgcaaa ctcagttcct cactgccgtt aagattgaga
     1921 ttgatggaaa aagccgaacc ctcctgagcc gagattgtat ttaacggaat tctcaggctg
     1981 atcccggacg taaagatccc gccttcgaat attcccggct cgaagcgaaa ctgccgcccg
     2041 ccccatataa gccccacaga atccttgatg ccccgcatat cggtgatctg aacggccata
     2101 aaggcttcgt tccaatgaat tctctccggg ttgagattta acctttccag cttggcaaag
     2161 gaaaaccgcc ccgccaactt cagtttgagg ttataaaccg ccgccttata aattccccgg
     2221 tagcggatct ccggccggat cttcccctca atccctagtt cctccggcaa gaactgggca
     2281 taatccatct cggttcgtac tttgccttga tcatccttaa acgacctctc ataaggaatc
     2341 gtgatcaccg gaccgccgac ggtttgggct tgagcccatt tcgaagtaat ctccgctacg
     2401 gcttctgctt tgcggatcga gcgttcaccg atgagtccat tgatcatcac tgtcggaatc
     2461 attaacacga gtatcaggat gccgatcatc aataagcgga caatgatcga attgcggttg
     2521 aaacctttaa cttccatttt gatttccatc tccattcatt gaattgattc gcatacacgt
     2581 ccgtccctta aataaacggt atcttcaacc atcccagacc ggaagttacc caccagtatc
     2641 ccccggcgat cagacccagc aggatgaagg ccggaatcat ctgacgaaaa tttagatttc
     2701 ctgccaggcg cagggttatt gccaggaata aagccttcca taaaaagatt aaaatgatca
     2761 gggtccaaaa tatcagcatg aaaaccgggg agcttgggaa tcgaccaaac cccgggatag
     2821 ttatgagtat atgggtgacg atattcaata gaaagaagag accggtcggc aggaagctga
     2881 acccccaaac tgcaaaaaag gaacgaagtt ttgtttcccg gaccagtatc cggcagatga
     2941 gatagatgat aaacaccgct gccggatagg agagaagcgg caacaggaat ccacaaaccc
     3001 aaaacgccgg ggacgactcc ctggtcaccg ccaaccatcc cagattgacc agcaacaatg
     3061 cacccacaaa cgtaatggtc gccgctttgg tgtttccgca gttaatcgtc tcttcaaaaa
     3121 aagcataggg ctgagaaata atccgccagg cttttcggta ccaagtttgc attgatgatc
     3181 ctcctatcct taccggacca aaagctgaat tagattaacc cgggccagaa ccccgtggaa
     3241 aagcgcgctt aacaaaaggg aagccgggag cagaagcctc caggggcggc tctttaaacg
     3301 cacaatcacc cattgataga tcacgaccga aagcggcaac tccgacagat gatactccaa
     3361 ccgttccgtc tccctgtgag cggtgtcaat catccgcgag acaatccggt tgtgccattg
     3421 tgcggctgct tcccggtcct ttctccggaa cagttcggtt ttttcgatta acctttgtaa
     3481 accctgctca ttcattcgtc tcttaacacc tccgtctccg gatccaaacg cttgtattcc
     3541 tcggcaaaag cccgccgcgc ccggaccagg atcgactcca ccgctttctc gcttcggcca
     3601 agccgaaccg caatctcccg tgtactaaac cctgtgaagt atttatcttc cagcacctga
     3661 cggtattcta agcttagctt taataatgcc tcccaaatca acaaattggt ctctgaatcc
     3721 gtctcggtgc aacaggttgc ggccagggtt tcatcgagcg gcaacatatt acgatccgtg
     3781 ttccgccggt aatagtcggc gatcttgaac ttcgcaatct gacacaacca tgtgaagacc
     3841 ctgctctgac ccttgaaacg cataattgcg gtaactgccc cggtcatggt ttccgccagg
     3901 atatcctcga tatcatcagg ttcccggacc cggcaatgaa tgaaggaatg taggcccggg
     3961 gcaaaccggc agtagaattc ttcaatcgcc gcttcatcgc cgtgttggat ctggcgaacc
     4021 agcgtcagat catcaatctc tggccagttt ttcatccgat tggattatct cctttctcga
     4081 ttaataagtc gtaattcgcc ggagaagtcc tgcgctcatt tttaaaaatt tcgattttga
     4141 atatttccc
//
LOCUS       DASVMB010000575         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_585216,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000575 DASVMB010000000
VERSION     DASVMB010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585216"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VF531_16150"
     CDS             complement(<1..675)
                     /locus_tag="VF531_16150"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7715552.1"
                     /translation="MKSWELLGQAIRDCFEGDLNAELLVESDCAGLERMPAALFFRNF
                     DQWSDLEQYAVNLCRGRILDIGAGAGSHSLELQERGFEVYPIDIAPQAVAVMRDRGLK
                     NASCVDFASYQAEPFDTILMLMNGIGVVGDLARLEVFLERIGGLLKPGGQILADSSDL
                     EYLRQVPDTDVSTVLTPGSEYGRIRYSFGYRGIQGEPFDWLFLDERTLRDYAGRTGWD
                     CQVLFCE"
     gene            complement(820..1857)
                     /locus_tag="VF531_16155"
     CDS             complement(820..1857)
                     /locus_tag="VF531_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX7715553.1"
                     /translation="MDQATIDGVVTHIVDLCRIPSPTGFTAKAVQYLQAELEKMGYEP
                     IRTRKGSVLVCLGGTGRGLAFAAHVDTLGAMVRTLKPNGRLRLTKLGGYPENAIEGEN
                     CIVFTRDGREFTGTIQCTQATPHVYHEPGKLERNDANMEVVLDEKVRSKEDLERLGVR
                     PGDFIAFDPRTVVTPSGFIKSRHLDDKAGSGILLALARWVKCSGVTLNRKVYLIFTVY
                     EEVGHGGAAGIPEDVEEMISVDMGAIGDDLQTDEYKVSICVKDSRGPYDYDVTTGLVR
                     IAAAAELNYATDIYPHYGSDVEAALAAGYDIRHGLVGPGVYASHGYERTHREALENTL
                     GLLAQYITEKR"
     gene            complement(1984..2787)
                     /locus_tag="VF531_16160"
     CDS             complement(1984..2787)
                     /locus_tag="VF531_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7715554.1"
                     /translation="MRRMDLELWEDKIPGYRQEIPQAKPSLKQFMPDSGSARGAIIVC
                     PGGGYEYKAAHEGEPIARWLNSLGLTAFVLDYRVSPYRYPIPVLDANRAVRWVRYHAE
                     NWHLDPNQIGLLGFSAGGHLAATVGTQFDNGQPDAADPVDRVSSRPDALILCYPVISL
                     GQYGHRGSIESLLGPNPSDTLRQSLSNELRVTTDTPQTFLWHTANDGAVPVENSLMFA
                     AALSRCRVPFAMHIFPDGHHGLGLADENPPVAVWTELCADWLKQIGFCD"
     gene            3308..>3687
                     /locus_tag="VF531_16165"
     CDS             3308..>3687
                     /locus_tag="VF531_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7715555.1"
                     /translation="MKRLVGYVRTYKWHFILAIAAMFLSIGLDMFNPRLQQAIVDDVI
                     LHGQMKEFGPLLLGLLGITLGRVIFGYVKEYAFDFGAQKVVASLRQNLFDHLETLSFT
                     FFDGMNTGELMSRLKEDVDSVWRAL"
BASE COUNT          927 a         1045 c          845 g          870 t
ORIGIN      
        1 ttcacaaaaa agaacctggc agtcccatcc cgtgcgtcct gcgtaatccc tcaaagttcg
       61 ttcgtcgaga aacagccaat caaaaggctc tccctgtatt ccccggtaac cgaagctgta
      121 acgaatccgg ccatactcgg aaccgggagt caaaaccgta ctaacgtcgg tatccggcac
      181 ctggcgcaaa tattccagat cggaggaatc ggccaggatc tggcctcccg gtttgaggag
      241 accgccgatt cgttccaaaa aaacttccaa ccgggccaaa tcgcccacca cacctattcc
      301 gttcatcaac atcaggatgg tatcgaaggg ttcagcctga taagaggcaa aatccacaca
      361 agaagcattc ttcaaacccc gatcccgcat caccgccacg gcctgcgggg cgatgtcgat
      421 gggataaacc tcaaatcctc gttcctgcag ttccaggcta tggctgcctg cgccggcgcc
      481 gatgtccaag atcctgcccc ggcaaagatt cacggcatac tgctcaagat ccgaccattg
      541 atcaaagtta cgaaaaaaaa gcgccgcagg cattcgttcc agaccggcgc agtcgctctc
      601 caccagcaac tcggcattca gatcaccttc aaaacaatcc cggatcgcct gtcccaggag
      661 ctcccagctt ttcatgaaca cttcaacctc gcttgatgat tctactattt tagtttgtgt
      721 aaagaattca gagcgcagaa aatacgtcga ggatttgggt tactctctct agcgcccgcc
      781 gctagagaga gcgagagtga gtcgtaggcc cgtaaagtcc tatctttttt cagtaatata
      841 ttgagccaaa agccccaagg tgttttctaa tgcttcgcga tgagtccgct catagccatg
      901 ggaagcatag actccgggcc ctaccaagcc gtgacgaata tcataccctg ccgcgagcgc
      961 cgcctccaca tccgaaccat aatgggggta gatatccgtg gcataattca gttccgccgc
     1021 agctgcgatc cggacgagtc cggtagttac gtcgtaatca tacggccccc gggagtcctt
     1081 gacacagata gagactttat actcatccgt ctgtaagtcg tcgccgatgg cccccatatc
     1141 cacagatatc atctcctcca catcctcggg gattccggcg gcccctccat gacctacctc
     1201 ttcatagacg gtaaagatca aataaacttt gcggttaagg gtcaccccgc tgcatttaac
     1261 ccaccgggcc aaagccagca atattccgga tccggcctta tcgtccaagt gccggctttt
     1321 aataaacccg cttggtgtca ccacggtccg gggatcgaag gcaataaaat ctcccggccg
     1381 gacccccagc cgttccagat cttccttgga gcggaccttc tcatcgagga ccacttccat
     1441 gttagcgtcg ttacgctcca gtttcccggg ttcatgataa acgtggggtg tcgcttgggt
     1501 acattgaatg gtccccgtga actctctgcc atcccgggta aaaactatgc aattctcacc
     1561 ttcgatggca ttttcgggat accctcctag tttcgtcaaa cgcagtctac cattgggttt
     1621 taacgtccga accatcgcgc cgagcgtatc cacatgggcc gcgaaagcaa gccccctccc
     1681 agtgcctccc aaacagacca gaaccgaacc ctttcgggtc cggataggct cataacccat
     1741 cttctctagt tctgcctgta aatattgaac cgctttggca gtgaaaccgg taggactggg
     1801 aatccggcat agatcgacga tatgtgtgac aacaccatcg attgtagctt gatcaatttt
     1861 caattttgga agtctccttt tgttaataca cctttcccct atcggctctt agctctcacc
     1921 tatcgaacca attaaaacac gaaattcacg aaaaaagaat ttacccaaac taaaaaacgt
     1981 gtttcagtcg caaaatccaa tctgcttgag ccagtctgcg caaagttcag tccaaacggc
     2041 aaccggcgga ttttcgtccg ccaaacccag accgtgatga ccatcaggaa agatatgcat
     2101 cgcaaacgga acccggcaac ggctcagggc agcggcgaac atcagactat tctctaccgg
     2161 aaccgcacca tcattggcag tgtgccagag aaatgtctgt ggagtgtccg tggtgacgcg
     2221 aagttcgttg gataaagatt gtcgcaaagt atcggaagga ttcggaccca acaaactctc
     2281 aatagaccct cgatgcccat attggccgag actgatcacc ggataacata gaattaatgc
     2341 atccggacgg gaactgaccc gatccaccgg atcagccgca tccggctggc cgttatcaaa
     2401 ttgtgtcccc acggtggcgg ccagatgtcc cccggccgag aaaccgagta aaccgatttg
     2461 gttgggatca aggtgccaat tctcggcatg ataccggacc cagcgaacgg cgcggttggc
     2521 gtccagcacc ggaatgggat agcgatacgg actaacccgg taatccagaa cgaaagcagt
     2581 caagcccaaa ctgttcaacc accgtgctat gggttctccc tcatgggccg ctttatactc
     2641 gtagccgcca ccggggcaga cgatgatcgc gccgcgagcg gaaccggaat ccggcatgaa
     2701 ttgttttaaa ctcggtttcg cttgaggaat ctcctgacga taacccggga ttttgtcttc
     2761 ccataattcc aaatccatcc tgcgcatggc gccatcaccc catcgaatct tttgataatc
     2821 aattcaaatt atatctcttt tctccttcgg caacctttct cctgcctcat aaatagccag
     2881 gcctccctgt cagccttatc cctgattggt tacgacaggg accggtgcct ttaaaaatac
     2941 tcaaaaaatt gtggtatttc tcttgacagt ggcctgatat cggttatata atgacttcat
     3001 tggaaaattt atcaagatag gaggtgggga tcgtgaaaat gcgtatcaat ttgtttctta
     3061 ccgcaataag gcacggcaat tttattcacg accatttgga tatccccgcc tcgattcgaa
     3121 tcggagctta atgcgccacc gatatgaata atggtaatca gaggccgcgg tgatatcaat
     3181 ccacggcctt tgtttttttg aatgtcaaag gccacggagt caatccctgt ggcctttttc
     3241 atctccataa actgtataca agatccaccc tttcggtcaa atctgttaaa gatgggatgg
     3301 taagcgcatg aaacgcttag tcggttatgt tcgaacttac aaatggcact tcatcctggc
     3361 aatcgccgcc atgttcctca gtatcgggct ggatatgttc aacccccgtt tacagcaggc
     3421 aatcgtggac gacgtgattt tacatggtca aatgaaggaa tttgggcccc tactcctggg
     3481 gctactcgga atcaccctgg gccgggtaat cttcggttat gtgaaggagt acgccttcga
     3541 cttcggcgcc caaaaggtcg tcgctagtct gcggcaaaat ctctttgatc acctggaaac
     3601 gctctccttc acttttttcg acggcatgaa caccggagag ctgatgtccc gtttgaaaga
     3661 agacgtcgat agcgtctggc gggccct
//
LOCUS       DASVMB010000576         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_216854,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000576 DASVMB010000000
VERSION     DASVMB010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216854"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..591
                     /locus_tag="VF531_16170"
     CDS             133..591
                     /locus_tag="VF531_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715556.1"
                     /translation="MRVRGILGTVWALLAIPIILATFIGNQGLAGSLVAATGVSISPR
                     ITGAEIVRAVDHGAYRTFIHRPVFDGLIGEESSGFVQIDWKAIDGRLPGHIVESIDYD
                     RDGADDFRIRIDTRTNRIQLVRVNQRVISVGQIYRFQNERVVRINLQNHQ"
     gene            651..1439
                     /locus_tag="VF531_16175"
     CDS             651..1439
                     /locus_tag="VF531_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7715557.1"
                     /translation="MAKHFWLTLTCLLIFSLPSFALASSKTMEYETLLLNDVGLFDPA
                     RLISAIAKQESSQSTMKNEKNEADYLSLVRLGIYYHNLAVGGYQKDYRGCAKKAYEIL
                     AKIIHEHQFPNELKPIAQPYLGSSRSMMGGETSNPIHKIGYVNEGITFLNEAVSKYQG
                     VSYLPRFIRANVFIALPKFFNKEEQAFQDYLALEEWNKADSSRIPKPIMAEVDYNLGN
                     CYKKRNKLDTAIEYWKIAYQFDREGAAGRKAKEMLKAFEAAYVE"
BASE COUNT          455 a          341 c          438 g          468 t
ORIGIN      
        1 atccaggaag cattggttcg ggttttttct tggattacca cgggaaaact gttttaaatc
       61 ccgtcggcaa gaccttgaat tggtaagttt ctgaggttgc ttgccgaatt atgttaaagg
      121 agtgaagtga tcatgagagt tcggggaatt ctcggaaccg tctgggcatt actcgccata
      181 ccgattattt tggctacgtt catcggcaac caaggtttgg ccggatcttt ggtcgcagcc
      241 acgggggtaa gtatttctcc ccggatcacc ggcgctgaaa ttgtccgggc cgtcgatcat
      301 ggtgcttacc ggaccttcat ccaccggccg gtctttgacg gtctgattgg cgaagaaagt
      361 tccggttttg tccagatcga ttggaaggcg atcgatggcc gtcttcccgg acatattgtt
      421 gaatcgatcg attatgaccg ggacggcgcc gatgattttc gaatccggat tgatacccgg
      481 accaaccgaa ttcaattggt acgggtaaat caacgggtta tttccgttgg gcagatttat
      541 cggtttcaaa atgagcgggt ggtgcggatc aatctgcaga atcatcaatg agcggcaggt
      601 tctttggcgg attggagaaa ccattttaat acaaaggagt tatgatgaga atggctaaac
      661 atttttggct gactttaact tgtttattga tattttcgct cccctcattc gcactcgctt
      721 catcgaaaac aatggaatat gaaacgttgt tattgaacga tgtcggcttg tttgatccgg
      781 cgaggctcat aagtgccatc gccaaacagg aatcgtcgca aagtaccatg aagaatgaaa
      841 agaatgaagc ggattatcta tcccttgtcc gcctggggat atactaccat aatctggcgg
      901 ttggcggtta tcaaaaggat taccggggat gcgccaagaa agcttatgaa atattagcta
      961 agatcattca cgagcatcaa ttcccgaacg agttgaagcc gatcgcccag ccttatctgg
     1021 ggtcaagccg ttcaatgatg gggggtgaaa cttcgaatcc gattcataaa atcgggtatg
     1081 taaatgaggg aataaccttt ttgaatgagg cggtctccaa gtatcagggt gtctcctatc
     1141 ttcccaggtt tatcagagcc aacgttttta tcgcgctgcc caagtttttt aataaagaag
     1201 aacaggcttt tcaggattac ctcgcgctcg aggagtggaa caaagcggat tcatcccgga
     1261 taccgaagcc cattatggcg gaggtcgatt ataacctggg gaattgttat aaaaaacgaa
     1321 ataagctgga tacggcgatt gaatactgga aaatagccta tcagtttgat cgggaaggcg
     1381 ccgcaggacg gaaagctaag gagatgctta aggcatttga agcggcatat gttgaatgat
     1441 tcattgaaac agcggtctat aaattctata caaaggattt cagttatgat aggataaata
     1501 cggcagtcgc cttttggctg cgccgtattt tattccctgg aaacaacgga gctgatgaga
     1561 taaattccgg gaagaaatat cctaacggag atatccttca ttatttagct ggggttgata
     1621 actgtgaaaa aaacggcatt gatcattatc gctgtagtgt ttgtgatctc cctgttttta
     1681 aaggggctcc ccggtcaaag ta
//
LOCUS       DASVMB010000577         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_293816,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000577 DASVMB010000000
VERSION     DASVMB010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293816"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VF531_16180"
     CDS             complement(<1..470)
                     /locus_tag="VF531_16180"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX7715558.1"
                     /translation="MKNRMETLKVPKYTLKRLPFYYRCTVNFFERGTQYVSSEDLARA
                     AQVNPVQVRRDLTLFRAMGTPGMGYNVPVLKQKLEELLGLKNVNEAVLVGYGRLGRAI
                     SDYSGFARYGLSIVAVFDNDPKVVGEHSGREVFPLAELPHIARRLRIKVGIITVP"
     gene            complement(582..>1926)
                     /locus_tag="VF531_16185"
     CDS             complement(582..>1926)
                     /locus_tag="VF531_16185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HEX7715559.1"
                     /translation="IVVAQVAPAVRVALGEEFGLPTGEIVTGKVVAALKRLGFDKVFD
                     TAIAADLTVIEETSEFLARLEKNQHLPQFTSCCPAWVKYAEHNQADFLPHLSSCRSPQ
                     QIFGSLVKRYWAKELGKDPGDIYVVSIMPCTAKKFEITRPEFSHDGVPDVDLVLTTQE
                     LGRMIREAGLVFDRLEVESFDLPFGFTTGAGVIFGATGGVAEAVLRAAYEMVTKEPLE
                     QMNFDEVRGFRGLKEASVDLKGQKVRVAVVHGLGNARKLLERIKTGEAQYDLIEVMAC
                     PGGCIGGAGQPINTAFEAKKARARGIYNADKLSQLRKSQDNPVVTRFYDKWLEKPGSP
                     EAHETLHTHYATRGRILGEEIQLLTSQADHRVDVAVCVGTCCYLKGSYDTLQAFVKQA
                     DEAGVKERLNLYATFCLEGCSASPSVRVDDEVIGGVAPQEVPRVFKEKVLNKLSR"
BASE COUNT          445 a          594 c          465 g          422 t
ORIGIN      
        1 ggcacagtga tgatacctac cttgatccgc aaacggcgcg cgatatgggg caattcggcg
       61 agggggaaaa cctcccgtcc gctatgttcc cctaccacct tagggtcatt gtcaaagacc
      121 gccacaatgc tcaaaccata gcgggcaaaa ccggaatagt cggatatggc ccggcccagc
      181 cggccatatc cgacgagtac cgcctcattc acgtttttaa gacccagcaa ttcttcaagt
      241 ttttgcttga gcaccggaac attgtacccc attccgggag tgcccatcgc ccggaaaagc
      301 gtgagatcac gccggacttg aaccggattc acctgcgccg cccgggccag atcctcggaa
      361 gagacatact gagtcccccg ttcgaaaaag ttgaccgtac aacgataata aaagggcaac
      421 cgttttaggg tatatttggg gacctttaat gtctccatcc tgtttttcac cttccaaacc
      481 ccgccgcaag catttgcggg ttttgattcg ctcgcaacgc tcagccgatt cccttgcagg
      541 gatagggtag atcgacccgg aattgggcat ttacccttaa ctcatcggct tagtttattg
      601 aggacctttt ccttaaagac ccggggtact tcttgcggag ccactccccc gataacctcg
      661 tcgtcaaccc ggaccgaagg gctggcgctg caaccttcca aacaaaacgt ggcataaaga
      721 tttaaccgct ccttgacccc cgcctcatcg gcttgcttta cgaaagcctg caaggtatcg
      781 taagagcctt tcaggtaaca acaggtaccg acgcaaaccg ccacatccac tcgatgatcg
      841 gcctgactgg ttaataattg gatttcttca cccaaaatcc tgccacgggt ggcataatgc
      901 gtgtgcaagg tttcatgagc ctccgggctc cccggctttt caagccattt gtcgtagaac
      961 cgggtaacca ccgggttatc ctgggacttt cgcaattgtg aaagtttatc ggcgttatat
     1021 atcccgcgag ccctggcttt tttagcctca aaggcggtgt tgatgggttg accggcgccg
     1081 ccgatacaac ctcccggaca agccatgact tcaatcaaat cgtactgggc ttccccggtc
     1141 ttgatccgct ccagtaattt acgggcattc cccaggccgt gaaccacggc cacccggact
     1201 ttctgtccct tcagatcgac gctggcctcc tttaaccccc ggaaaccacg gacctcatca
     1261 aaattcatct gctctagcgg ctcttttgtg accatttcgt aggcagcacg cagcaccgcc
     1321 tcagccacgc cgccggtggc gccaaaaatg acacccgccc cggtggtaaa cccaaagggg
     1381 agatcgaagg actccacttc caatctgtca aagaccaaac ccgcctcacg gatcatccgc
     1441 cccagctcct gggtggtcaa gaccagatcg acatcgggga ctccgtcatg gctaaactca
     1501 gggcgggtga tttcaaactt cttcgcggtg cagggcatga tcgataccac gtagatatcc
     1561 ccgggatcct tgccaagctc cttggcccaa taccgtttga ccagcgagcc gaagatctgc
     1621 tgtggtgaac ggcagctgga gagatgcggc aggaaatccg cctgattatg ttcggcgtat
     1681 tttacccaag ccgggcaaca ggaggtgaac tgcggcaaat gctgattctt ttccaaccgc
     1741 gccaaaaatt cgctggtctc ctcgatcacc gtcaaatctg cggcgatggc ggtatcaaaa
     1801 accttatcaa atcccaaccg ttttaaggcg gccactactt tgccggtgac gatctccccc
     1861 gtcggaaggc cgaactcttc tcccaaggcc acccggaccg ccggagccac ctgagccacg
     1921 acaatc
//
LOCUS       DASVMB010000578         8017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_511231,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000578 DASVMB010000000
VERSION     DASVMB010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511231"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF531_16190"
     CDS             <1..408
                     /locus_tag="VF531_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7715560.1"
                     /translation="NLTVGENIETVSNISKSPLDIDEVLGAVGMKDKKHRFPRELSGG
                     EQQRVSIARAIVKNPKLLLCDEPTGALDFQTSRSILKLLQEVNKKYGTTILMITHNAA
                     IADMANRVFKLRNGEIAEELVNEKIIPAERIEW"
     gene            402..2654
                     /locus_tag="VF531_16195"
     CDS             402..2654
                     /locus_tag="VF531_16195"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7715561.1"
                     /translation="MVMVINKRIKRVYLANKAQYIGAVLLIVLSCFLFTNLVLVSNNL
                     TRLMNEYEKDYVQEDASFTTDKSMDNLPELESASDAIIEEGKSFDYALSEGSTLRVFT
                     KNDKINIPAIIEGNELNGSGEILLNPAFAVAHRYKIGDVLTILDKPFTVAGFMALPNY
                     IYPLKLETDLLYSPQDFGIAVIGKEDFAALGKEGSFYAVKFNHPEENARTQSMKFIEL
                     LKSRGIGITQWTDIGVNIRVSGADAKFSSASLMGRAMPTAFFLLTAILLGNVIGRMIE
                     RESVIAGALYALGYKRRELYVHYLKFPLVVAAIGGVLGTILGLFTVRPMMSVYFTLFD
                     IPMTGIDYSQAVIILSLLLPVLFLGLSSFIVIHKELKHSPAELMKGNKEKGKVNFLER
                     GFKLEKLKFALKFKIREQFRSLSRLALLLVGCSVATLLLLFGFFLKSGMDYFLKIEVE
                     NAQNYQYEYVFDSLRTKPVPADAEPFAVSKFLLPGNDNKVFHVAGYLPDSNISTVVDE
                     SGNKIRMDQVIITKPIADKLSVKPGDTITVVRKSDFQEFSLTVGNIADSLNMTIMMPL
                     TEYNGKFGMPEGSYNELFSNELLDIPENEQYKVYSIEDKISAIQSLMAPLASMVVFIA
                     AIAFIIGMIIIYVVTSMIVEENKSTISLMKIFGYRRKEVNSLILGSSTIVVVIGYILG
                     ILLSFLSVGGLIKALEDSLTFAMPVTIAPLYVIFGFIVVMLSYELSKLLCRRKVSAVS
                     MSEALKAGTE"
     regulatory      2693..2778
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2831..3142
                     /locus_tag="VF531_16200"
     CDS             2831..3142
                     /locus_tag="VF531_16200"
                     /inference="COORDINATES: protein motif:HMM:NF039645.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6075 family protein"
                     /protein_id="HEX7715562.1"
                     /translation="MKNPAPYGLFDCGFAPDFSEAIRPRYPEYCRNMQSVRMCSATPP
                     QDCRNWWRILEHCRLAEAIRNTFLQTGRVVKSPVGAFTILPVFLEGKTLGTYAGPKHV
                     K"
     gene            complement(3107..3274)
                     /locus_tag="VF531_16205"
     CDS             complement(3107..3274)
                     /locus_tag="VF531_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715563.1"
                     /translation="MKATTTSLTVTGVGTAVSGVGAALLRSKWGAGILGFGLAHVVLG
                     LLDMFRPSVRT"
     gene            3453..4493
                     /locus_tag="VF531_16210"
     CDS             3453..4493
                     /locus_tag="VF531_16210"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7715564.1"
                     /translation="MSTHMLEEIKEQPGVIVRTMQQNTATLRDLTENLKRRPVHSIMV
                     AARGTSNNVAIFAKYFGEYFLGMPVSTAASSIINLYHRRMRLDSVLILAISQSGEGPD
                     IMGVVKEAKNQGALTVGITNHEKSSLAREVDFPIITGAGPEQSIVATKTCTASMMAIA
                     SLIQNWSGERDFGAQLEKVPELLTKIIAREAEIAEKAYLFKVFEDCVILARGLNYCAA
                     LETAFKIQETCYLNAQGYSAADILHGPIAMLDDHFSALVYACQGPSLPGIVGTISRLK
                     QIGIPLLLIANEPALLNENRLNFQIQEEFSEEIMPFASVVFGQIFAYYLAQLKGRNPD
                     FPRRLKKVTQTL"
     gene            4739..5587
                     /locus_tag="VF531_16215"
     CDS             4739..5587
                     /locus_tag="VF531_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7715565.1"
                     /translation="MAADQCILKIRSLYNSFSKTEKKIADYVFENTNRIIYMSVTEFA
                     ENCDVGETSIIRFCRTIGLKGYQEFKLVLARECINPQENLHERITEDDAMEEIISKIT
                     AVNLQAIQNTSRVLSLSALGKAIDAILRADKVDIYGVGASAFTAGDAKYKLMRIGIKC
                     EFFSDPHLQCMSAVNLTKKSVAIGISFTGSTKDTVDSLSLAKKAGAFTIAITNYEKSP
                     ITKYANVVLLTSAEETPLRSGALTSKIAQLQVLDILYTGIALKLGDKAYELLNETAKA
                     VLPKLY"
     gene            complement(5780..7309)
                     /locus_tag="VF531_16220"
     CDS             complement(5780..7309)
                     /locus_tag="VF531_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019685890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HEX7715566.1"
                     /translation="MKNILYIPLDERPCNYRFMPLIAEGTDYHVLIPPEEYLGHKKNP
                     ADTAALWDWIFQNAASADGAILSIDMLVYGGIVPSRLHRLSIDQCVRSLDNIKKLKAR
                     NSHLTIYANSLIMRCPAYSSNDEEPDYYQDWGKDIFDWGVIRQKSDLNLSSPEEAAQL
                     REIIGRLPDEVMRDYTERRKINRSVNQRVIDLVRDGVIDFLVIPQDDSAPFGFTAMDQ
                     QFLRGYILQNHLGLKVFMYPGADEVGMTLFARMIYRGAKSKLNVYVRFASVSSPFIIP
                     RYEDRLLYESIKSQLLCAGGFICQSLEDADLVLMVNAPAAKMLEAWEQDHIGADYNVN
                     RNLMEFIEYSEYIVNTRKIPCAIADVAFSNGGDLELVEYLRQKKLLFKLAAYAGWNTS
                     SNTLGTAITQAFIYHRYGASQSHLNFLGLRYAEDVAYCSHVRKNVTDHLPAGTDLSYF
                     KIDGPRGTVAETVKNELQQFLENLIDDDDYRVRIKDIYMPWSRMFEVGLDVKVESNDR
                     Q"
     gene            complement(7366..>8017)
                     /locus_tag="VF531_16225"
     CDS             complement(7366..>8017)
                     /locus_tag="VF531_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7715567.1"
                     /translation="VFKLPVMVENDVNAAALGEHWLGAAKQFRDFICLTLGTGIGGAI
                     FRNGTIDHGWGGISGEFGHMSIAYDGLPCNCGNKGCFEQYGSVSALINNLLRRIHDGA
                     PSFIPDMVQGDLSRVNGELIFAAKEKGDRLAAEVIDEYIAYLATGIVNLVHILNPGAV
                     VMGGGITNVGEALMQPLRQAVKAGAMPKFTENLAIIRSELGDYAGLYGAVKNLLTS"
BASE COUNT         2103 a         1851 c         1957 g         2106 t
ORIGIN      
        1 aaccttacag tcggggaaaa cattgaaacg gtttccaata tcagcaaatc gcctctggat
       61 atcgacgagg tattaggggc ggtgggaatg aaggataaaa agcaccgctt ccccagggag
      121 ctttccggcg gggagcagca acgggtatcc atagccagag caatcgtcaa gaacccgaag
      181 ctgctgcttt gcgacgagcc caccggcgcg ctggattttc agacctcccg cagcattctc
      241 aaactcctgc aagaggtgaa caaaaaatac gggaccacca ttttgatgat cactcacaat
      301 gccgccattg ctgacatggc gaaccgggta ttcaagctca ggaacggtga aatcgcggaa
      361 gaattggtga atgagaaaat tatcccggcg gaaaggattg aatggtgatg gtaattaata
      421 agcgaatcaa acgtgtttac ctggcgaata aggcgcaata tatcggcgct gtattactca
      481 tcgttttaag ctgctttctg tttacaaacc tggttctggt aagcaataac ttaacgcggc
      541 tgatgaatga atatgaaaaa gactacgtgc aggaagacgc ttcctttaca acagacaaaa
      601 gcatggacaa tttacctgag cttgagtcgg cttcagacgc gatcatagaa gaaggcaaaa
      661 gcttcgatta cgcgctttca gaagggagta cgctgcgtgt tttcactaag aacgataaaa
      721 tcaatatccc ggccatcatt gaaggcaacg aactgaacgg aagcggagaa atcctgctca
      781 atccggcttt tgccgtcgcc catcgctata aaatcggcga tgtactgacc atattggata
      841 agccgtttac ggtcgccggt tttatggctc tgccgaatta tatttatccg ctgaaattgg
      901 aaacggacct tctgtattct ccgcaggact tcggcattgc agtgataggt aaggaggatt
      961 ttgccgcact tgggaaagaa ggcagtttct acgcggtgaa attcaaccat cctgaagaga
     1021 atgcccgcac tcagtctatg aaattcatag aactgttgaa aagccgcggc attggtatca
     1081 cacaatggac agatatcggt gtaaatatta gggttagcgg ggccgatgct aagtttagtt
     1141 cggccagtct tatgggcaga gcaatgccta ccgcgttctt tttgttgact gctattttac
     1201 tcggcaatgt tattggtcgg atgatcgagc gcgaatcggt gatcgcaggc gcattgtatg
     1261 cgttagggta taaaagaagg gaactctatg tccattattt gaaatttcct cttgtggtcg
     1321 ctgcaattgg gggcgtcctt gggactatcc ttgggttgtt taccgtacgt ccaatgatgt
     1381 cagtttattt tactttgttc gacataccca tgaccggaat cgattatagc caggccgtca
     1441 taatcctgag ccttctcctt cccgttttat ttcttggatt gagcagcttc attgtaatcc
     1501 ataaagagct taaacattcg cccgccgagc tgatgaaagg gaataaagaa aagggcaagg
     1561 taaatttttt ggagcgtggg tttaagcttg aaaagctcaa attcgctttg aagttcaaaa
     1621 tccgggaaca gtttcggagc ctatccagac tcgccctgct gctcgtcggc tgctctgttg
     1681 cgacattgct tcttcttttc ggttttttcc tgaaaagcgg tatggattat ttccttaaaa
     1741 ttgaagtgga gaacgcgcaa aattaccagt acgaatatgt atttgacagc ctgcgcacca
     1801 agccggtgcc ggctgacgcc gaaccttttg cggtatcgaa atttttgctc ccgggtaacg
     1861 ataataaagt ttttcatgtc gccggctacc tgccggattc caacatatct acggtggtag
     1921 atgaatccgg caataagatc cgcatggatc aggtgattat cacaaagccg attgctgata
     1981 aattaagtgt taagcccgga gatacgatca ccgtcgtccg taaatcggat tttcaggaat
     2041 tttctctgac ggttggcaac atcgcggatt ccttaaacat gactattatg atgccgctta
     2101 cggagtataa cgggaagttc gggatgccgg aaggaagcta taacgagttg ttcagcaatg
     2161 agctgctgga tattccggaa aacgagcagt ataaagtata ttcgatagaa gataagatat
     2221 ctgcgataca aagcctcatg gcacctctgg cgtcgatggt cgttttcatt gcggcgatag
     2281 cctttatcat cgggatgatc atcatctatg ttgtaacctc tatgattgta gaagaaaata
     2341 agagcacgat ttcactgatg aagattttcg gatataggag gaaggaagta aactccctga
     2401 ttctgggcag ctcaacgatt gtcgtcgtga ttgggtatat ccttggcata cttcttagtt
     2461 ttttatcagt gggaggactc ataaaagcac ttgaagacag ccttactttt gcaatgcccg
     2521 tgacgattgc tccgctttac gtcatctttg gatttatcgt tgtcatgctc tcctatgagc
     2581 tgtcgaagct gctttgccgg agaaaggtca gtgcggtttc catgagcgaa gccttaaaag
     2641 ccgggacgga atagaaaagt ttgttgagag taaaataaga catttgaatt attcgtgtta
     2701 agggcaaact tgccgaaagg caaggactca aagccgaggt ctaagatgcg agtgcattat
     2761 gacagcctga ctgccgcaaa ggattgcgta ctaaacctgc cctttttgcg gcagtttttt
     2821 ctacatataa attaaaaatc ccgcacctta cgggctattt gattgcggtt ttgcaccgga
     2881 cttttctgaa gcaattcggc cgcgctatcc tgaatactgc cgcaacatgc aatccgtgcg
     2941 gatgtgttcg gctacaccgc cccaggactg ccggaattgg tggcggattc tggagcactg
     3001 caggttggcc gaggctatcc gtaatacttt tttgcagacc ggaagagttg tgaaaagccc
     3061 ggttggtgcc ttcacaattc ttccggtctt tttggagggc aaaactttag gtacgtacgc
     3121 tgggccgaaa catgtcaagt aatccgagga ccacatgggc cagcccgaaa ccaaggatgc
     3181 ccgctcccca tttactcctg agtagtgccg ccccaacgcc ggagaccgcg gttccgacac
     3241 cggtcacagt cagactggta gtagtcgcct tcaaagcttt ccacctccta aagtatagta
     3301 tcccctttaa actcccgggt taaccacgaa gtcggttcag gtcttaaatt cgtttccgag
     3361 tcttgatacc cagattgcaa tatattataa tttaaatagt tgttaaaacc ttgtaaaacc
     3421 gataatgttc gggaatcttg gaggcagcgg ttatgagtac ccatatgttg gaagagatca
     3481 aggaacagcc aggagtcatc gtcaggacca tgcaacagaa taccgctacc ctccgggacc
     3541 ttaccgagaa tctaaaaagg cgtcccgttc attcgattat ggttgctgcc aggggtacat
     3601 cgaataatgt ggcaattttc gccaaatatt tcggggaata ttttctcggt atgcctgtat
     3661 ccaccgctgc ctcctccata attaaccttt accatcgccg gatgagactg gactcggttt
     3721 tgatcctggc catatcccaa tcgggagagg gaccggatat tatgggagtg gtaaaagagg
     3781 ccaagaatca gggggcatta acggtcggga ttacgaatca cgagaaatct tcgctggccc
     3841 gggaggtcga ttttccgatc attaccgggg cagggccgga gcagagtatt gtagccacta
     3901 agacttgcac cgcttcaatg atggcaatcg catcattaat ccaaaattgg tccggtgagc
     3961 gggatttcgg tgcccagctg gaaaaagtcc cggaattatt gacaaagatc atcgccaggg
     4021 aagcggagat tgccgaaaag gcatacttat tcaaggtctt tgaggactgc gttattctgg
     4081 cacggggcct taactactgc gccgccctgg agacagcctt taagattcag gaaacctgct
     4141 atctgaatgc ccagggatac tcggctgcgg atatcctgca tggacccatc gcgatgctgg
     4201 atgatcattt ttcagcgttg gtctatgctt gccaggggcc gtccctgccc ggaatcgtcg
     4261 gaaccatctc ccggttgaaa cagatcggca ttccgctgct attgatcgcc aatgaaccgg
     4321 cattattgaa cgaaaaccgc ttgaacttcc agattcaaga ggagttttcc gaagaaatca
     4381 tgccgtttgc ctctgtcgtc ttcggccaga tcttcgccta ttacttagcc caactaaaag
     4441 gccgcaaccc tgattttccg cgccgtctga aaaaagtcac ccaaacctta taaattttca
     4501 agttcagcct ccaaacccgg atgcgctact ttccaaaaga aaaatagcgc ttttttgttt
     4561 ggtggatgat gactttcttc gccgtcagct atagaattag ctcaatggat tgttttgttt
     4621 cgaaagacca tagaaaaatt atctatctca tgtataataa aggagacagt gaatttataa
     4681 gtaaaatgtc aaaccgggct agggtacttt tgataatttt tgttttggag ggtgtctcgt
     4741 ggcggccgat caatgcattt taaaaatacg gagtttgtat aatagttttt ccaagacgga
     4801 gaagaaaatc gcggactatg tttttgaaaa tacgaatcgg atcatctaca tgtcggtaac
     4861 ggaattcgcg gaaaactgcg atgtgggaga aacatccatc atcaggtttt gtagaaccat
     4921 aggtttgaag ggatatcaag aattcaagct ggttcttgcc agagagtgta ttaatccgca
     4981 agagaactta catgaacgaa ttaccgaaga cgacgcgatg gaagagatca ttagcaaaat
     5041 aacagccgtc aatcttcaag cgattcagaa cacttccagg gtactctccc tgtctgctct
     5101 cggtaaagca attgatgcga ttttaagggc agataaagtt gatatttacg gagttggagc
     5161 atcggccttt acagccggcg atgccaagta taagctaatg cgaatcggga tcaaatgtga
     5221 attttttagt gatcctcatt tgcagtgtat gtcggcggtt aatctgacta aaaagagtgt
     5281 agccattggc atcagcttta ccggtagtac caaggatacg gtggattcat tgagtcttgc
     5341 caaaaaagcg ggggctttca cgattgccat taccaattat gaaaagtcgc ccatcacgaa
     5401 atatgccaat gtcgtattac tgacttccgc cgaggagacg ccgctgcgca gcggagcact
     5461 gacttcaaag atagctcagc tccaggtcct tgacatcctt tacaccggaa tcgctttaaa
     5521 gctaggcgat aaagcctatg agctgctcaa tgaaacggcg aaagcggttc ttccaaaatt
     5581 gtattagtac taggcaacag ggaacaggca ataggcaaca gggagcaagg actaggcaac
     5641 ttgggacagg caataggcaa caggaaaaag ctgaaagcca acatctagaa caacatttcc
     5701 ccagctacac ccttaagaga gttcgctttt gggtttttaa ctaaaccacg tttttctcct
     5761 attgcctgtt ccctatcccc tattgcctat cattcgattc tactttcaca tccaacccca
     5821 cttcaaacat tctggaccac ggcatgtata tatccttgat ccggactcgg tagtcatcat
     5881 catcgataag gttctctaag aactgctgca actcgttttt gaccgtttcc gccactgttc
     5941 cccggggtcc gtcgattttg aagtaactca gatcggtacc ggcgggcaga tgatcggtga
     6001 cgttttttct cacatgagag cagtacgcaa catcctccgc gtatctcagg cctaaaaaat
     6061 tcaggtgcga ttggctggca ccgtatctat gatagataaa cgcctgggtg attgcggttc
     6121 caagtgtatt ggagcttgta ttccaaccgg cgtaagccgc aagcttgaat agaagctttt
     6181 tttggcgcaa gtactcaacg agctccagat cgccgccgtt tgaaaaagcc acatcagcaa
     6241 tggcacaagg tatcttacgc gtattgacga tgtattcgct atactcgata aactccatta
     6301 agttacgatt gacgttgtaa tccgcaccga tatgatcctg ttcccacgcc tccaacattt
     6361 tggcggccgg cgcgttcacc atcaatacca gatcggcatc ttcaaggctt tggcaaatga
     6421 accccccggc gcaaagtaat tgagatttga tgctttcgta gagcagtcgg tcttcatagc
     6481 gggggatgat gaacggactg ctgacactgg cgaatcgtac ataaacgttt aattttgact
     6541 tggcaccgcg atatatcatt cgggcgaata aagtcatacc gacctcatcg gctcccggat
     6601 acatgaaaac cttgagacct aaatggtttt ggagaatgta gcctctaaga aactgttgat
     6661 ccatcgctgt gaaaccaaaa ggagccgaat catcctgggg aataaccaaa aagtcgatga
     6721 cgccgtcccg taccagatcg atcacccttt ggttgacgga gcggttaatc ttccttcgct
     6781 ccgtatagtc cctcatcact tcatccggaa gccgtccgat gatttctcgc agttgagcgg
     6841 cttcttccgg tgaagagaga ttcagatcgg atttttgacg gataacgccc cagtcgaaaa
     6901 tatccttgcc ccaatcttga tagtaatcgg gttcttcgtc attacttgag taagcggggc
     6961 atctcatgat tagactgttg gcgtagatgg tgagatgact gttgcgcgcc tttagtttct
     7021 tgatattgtc gagactccgg acgcactggt ctatggagag acgatgaagc cttgatggga
     7081 caatccctcc gtataccaac atatcgatgg ataggatagc cccatcggcg gaggcggcgt
     7141 tttgaaagat ccagtcccac aatgctgcgg tgtctgccgg gtttttcttg tggccgaggt
     7201 attcctcggg tgggatcaga acatgataat cggtgccttc ggcaatgagc ggcatgaacc
     7261 tgtagttgca tggtctttca tcaaggggaa tatagaggat gtttttcatg gtatgacctc
     7321 cgggatattt attgagtcat gaggatatcg ggtttttcga aatcgtcatg atgtgagtag
     7381 attcttgacg gcaccgtaca ggcccgcgta atcacccagt tcggaacgga tgattgccag
     7441 gttttcggtg aattttggca ttgctccggc ctttacggct tgacgtaatg gctgcatcaa
     7501 tgcctcgccc acgttggtta ttccaccacc catcacgact gcccccggat tcaggatatg
     7561 gaccagattg acgatgcctg ttgccaggta ggctatatat tcatcaatca cttccgcggc
     7621 gaggcgatcc cccttttcct tggctgcaaa tatcagttcg ccgttaaccc gggacagatc
     7681 tccctgtacc atgtcgggga tgaaagatgg ggcaccgtca tgaatccggc ggagcaagtt
     7741 attgattaag gccgaaacgg atccgtattg ttcaaaacag cctttgttgc cgcagttaca
     7801 tgggaggcca tcatacgcga tgctcatatg gccgaattcc ccggagatcc cgccccaacc
     7861 atgatcaatg gtgccattcc ggaatattgc cccgccaatg cctgtcccca gcgtcaggca
     7921 gataaaatct cggaattgct tggccgctcc cagccagtgt tcacctaggg cagccgcatt
     7981 gacatcgttt tccaccatga ccggtagctt aaaaacc
//
LOCUS       DASVMB010000579         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_732197,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000579 DASVMB010000000
VERSION     DASVMB010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732197"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF531_16230"
     CDS             <1..203
                     /locus_tag="VF531_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016937779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX7715568.1"
                     /translation="NRLEHTINNLGVASENITASESRIRDVDMASEMMSYTKLNVLSQ
                     AAMSMLAQANQQPQQVLQLLGR"
     gene            629..970
                     /locus_tag="VF531_16235"
     CDS             629..970
                     /locus_tag="VF531_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715569.1"
                     /translation="MNSQESTEVVSTYNEYLERLDSATRHFSEDLEASNFRVIGEVLP
                     AIIEGLEWVNEAASRFVTLQKVKPEIYQNFQNTITTMGDALENKDYPLLHDVLEYDLL
                     PIFKEMKVVMN"
     gene            991..2205
                     /gene="fliB"
                     /locus_tag="VF531_16240"
     CDS             991..2205
                     /gene="fliB"
                     /locus_tag="VF531_16240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HEX7715570.1"
                     /translation="MDNVLMPTYMTGFRCSGGECPHNCCDEPWIIAVEDRISQKYRQI
                     QDPQLAPLFQRYIQKIDDPKITGFSRIEKLPGGKCPFQAASGYCIIQERYGTECLSGV
                     CNTYPRHINKVNGVFEISGKISCPEVCRTALFTPEPITFQSTQSTTCIDPKAAYVFNT
                     INFTAEPISASEILALRDFTIRILQERQCQLIDRLVFLGLFYKKVKESLQTGKNSDIS
                     GLIDNYRQTLETGGARGLTSGIAANYPLKLELILAICNIKAKNDVHYGGYFQTILAGN
                     GYFRGTSDERVQNYSRINRDFFAGPMRSYEYILENFLVNHTFQNFLYFKSGAIDQFHA
                     ELIVHFVLVQFHLAVILGPSQEVDEATFGETFRQYSRYWEHDRNTMEKIGAFLRERSL
                     VTLPYLTVLMKD"
     gene            2233..>4571
                     /locus_tag="VF531_16245"
     CDS             2233..>4571
                     /locus_tag="VF531_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HEX7715571.1"
                     /translation="MLFDKNSKLLKEKFPHLALELKEPGHTGVEFEVIQSKTGLPVAR
                     VIRNNRRVFLNSPYDPVSEADRWLGSLEEKKDDYLLVGGSGFLYHLKALSGRRNFKRI
                     IVYEPCREVFLSCLHEVDWEPLLKGLDFLLVVGDAHQDNAQLLIDYLGASYFGDLKSS
                     LGVTLLPAYRELFSGEIAAFQEKITETLRLIRVNLDTNELFAKQWLVNGLKNLNSMAG
                     SPVIKDYFHQFSGVPAIIVSAGPSLEKNIHLLNAVKEKAVIICAGTSIRAMLHHGVRP
                     HFLVGFDGGQVNRLVYNDLDLHDINLVYSSRFFHEIVTNYNGPKILMKNDVETFPDLL
                     ALTQGMEIGTVRSGFSVAHTCLDLAAKLGCNPIVLIGQDLAYTDNKHYAEGQVASFQN
                     LVDNQLVIRKDFEGRDIVTSKKFESFKFVFERIIAELDPGIRVINATEGGLPITGAPN
                     RKLDQVLAEHCQMEREISQRIRVIYERGLQDTKRRRMDFNSIALKLKTLVNQGITGME
                     GLIGRLQELRRVTFSEAFDRNKLLPAFQQIASAYEMVVSRREFDILLKSLRDSKLLVI
                     KMRMAEVNMVATREEFDGILQSSLLFFSETQKYLEYIRDCMKDIVPAAVKEEQSSVSF
                     GLNSETCRSFEGRIKRGETLNEIQRTLDRVISWGSNGDEMARALYLRGLISIRSNQLG
                     KAVIDLEQAVALDKSLAKAYFMLFRLSQKGKDLVTACTYLERCRHLNYRTEYCLRRQI
                     KNYYLIKDYVAVNNFITEHREKLTPRRFFSILLGECLSF"
BASE COUNT         1233 a         1000 c         1155 g         1183 t
ORIGIN      
        1 agaaccgttt ggagcacacc atcaacaact tgggcgtcgc ctccgagaac atcaccgctt
       61 ccgagtcccg aatccgcgac gtcgatatgg cttccgagat gatgagctac accaaactca
      121 atgtcctgtc tcaggcagcc atgtccatgc tggcacaggc caatcaacaa ccgcagcaag
      181 tattgcaatt gctcggtcgg taagcgtcaa ggttacttct caaaagactc tccttcggga
      241 gggtcttttt ttacgcgccc ccaggggcgc gaggggacag ggaataggga acaggcaata
      301 ggcaaaagaa attttgtggg aaaaggggtg gaggcccaaa cccgaccctg tcctttaaag
      361 ggaactagta atgcgttagg attttttttg aggtaaattt gcagttattt ttcaatataa
      421 atcgtaaatc ggaactaacc atgctcatac cagagagtat tgatggcttc aggaatgagc
      481 atacacacca ctgttgcctg ttgcctagta cctattgcct ccatacatat tgaaccctgt
      541 tttttaaaca cccctaaaga tcccaggcaa aacgccgatt aatgatatag aaatgaagcc
      601 ggtttcgaca atattctgga ggtttattat gaattcacaa gaatctaccg aggttgtgtc
      661 gacatacaac gagtatttgg aacggctcga ctccgctacc cggcatttct ccgaggatct
      721 ggaagccagc aactttcggg tgatcggcga ggttctgccg gcgatcatcg agggattgga
      781 atgggtgaat gaagcggcta gccggtttgt caccctgcaa aaggttaaac cggagatata
      841 tcagaatttt caaaatacca tcacaacgat gggcgatgct ctcgagaaca aagattatcc
      901 cctccttcat gatgtgctgg agtatgactt gctgcccata tttaaagaaa tgaaggtcgt
      961 tatgaattaa gcgatggatg gagtatattg atggataatg ttctcatgcc aacatatatg
     1021 accgggtttc gttgcagcgg cggtgaatgc ccccataatt gctgcgatga accctggatc
     1081 atcgctgttg aagaccggat tagccaaaaa taccggcaga tccaggatcc tcaactggca
     1141 ccactctttc agcgatatat tcaaaagatt gacgatccca aaatcaccgg attttccaga
     1201 attgagaaac ttcccggcgg caaatgtcct ttccaggcgg caagcggcta ttgtattatc
     1261 caagaacgct acggtaccga atgcctctct ggagtatgca atacctaccc aaggcatatc
     1321 aacaaggtga acggggtatt cgaaatctcc gggaagatct cctgcccgga ggtatgccgt
     1381 acagccttat tcaccccgga acccataacc tttcaatcaa ctcagtcgac aacgtgtatt
     1441 gatcccaaag ccgcctatgt attcaataca atcaatttta ccgctgaacc gatatcagct
     1501 tcggaaatac tagcgttaag ggattttacg atccggattt tacaggaaag acaatgtcaa
     1561 ttgattgacc gccttgtttt tctgggttta ttctataaaa aagtaaagga gtcccttcag
     1621 actgggaaaa acagcgatat ttccggcctg atcgacaact accggcaaac gctggaaaca
     1681 ggcggcgccc gggggctgac ttccggaatt gccgccaatt acccgttgaa attagagctg
     1741 atcctggcaa tctgcaatat caaggcgaaa aacgacgtcc attacggggg ctattttcaa
     1801 acaatactgg cagggaacgg atatttccgg gggacttcgg atgaaagagt tcaaaactat
     1861 tcccggatca accgtgattt tttcgcgggt ccgatgagga gttatgagta tatcctggag
     1921 aacttcctgg tgaaccatac ctttcaaaat ttcctgtatt tcaagagtgg cgccattgat
     1981 cagttccatg ctgagcttat tgtccatttc gtactggttc aattccattt ggctgtgata
     2041 ctcgggccca gtcaagaagt cgatgaagcg acgtttgggg agacattccg gcagtattcc
     2101 cgttattggg agcacgaccg gaatacgatg gagaagatcg gagcttttct acgggagcgc
     2161 tccttagtga ccctgccgta tttaacggtg ttgatgaagg attagtatac ggatcaaaaa
     2221 agggtgagcg ttttgctgtt tgataaaaat tcgaaactgc taaaagaaaa atttccacat
     2281 ctcgcattgg aactcaagga acccggacac accggtgttg agttcgaagt cattcagagc
     2341 aagaccgggc ttccggtagc aagagtgatc cgcaacaacc ggcgagtctt tttaaacagc
     2401 ccctatgatc ctgtgtcgga agctgaccgc tggctgggct cgcttgaaga aaaaaaagac
     2461 gattatctcc tggttggcgg gagtggcttt ttgtatcact taaaagcctt aagtggtcgg
     2521 agaaatttta aacggatcat cgtgtatgaa ccctgccgcg aggttttcct ctcctgtctc
     2581 catgaggtgg attgggaacc tctcttaaag ggtttggatt tcctgttggt ggtgggcgac
     2641 gcacatcagg acaatgccca actgcttatc gattatctcg gcgcatccta ttttggtgat
     2701 ctgaagtcga gtctgggtgt tactctttta ccggcttacc gtgaattatt tagcggtgaa
     2761 attgcggctt ttcaggaaaa gatcacggaa acgctccggc tgatccgggt gaatctcgat
     2821 accaatgagc tttttgcgaa acagtggttg gtaaacggtc ttaaaaatct gaattcaatg
     2881 gccggttccc cggttatcaa ggattatttt catcagttct ccggagtgcc ggcgattatc
     2941 gtctccgccg ggccttccct ggaaaaaaat attcatctcc tgaacgcggt taaagaaaag
     3001 gctgtgatca tctgtgccgg aacatccatc cgggcaatgc tgcatcatgg ggtgcggccc
     3061 cattttttgg tgggatttga cggcggccaa gtcaatcgcc tggtatacaa tgatcttgat
     3121 ttgcatgata tcaacttggt atatagttca cgattcttcc atgagatagt aacgaattat
     3181 aacgggccga aaatcttaat gaaaaatgat gtcgagactt ttcccgattt attagcattg
     3241 acacagggta tggagattgg aacagtcaga agcggatttt cggttgcgca tacctgtctc
     3301 gatttggcgg cgaaattggg gtgtaatccg attgtgttga tcggccagga tctggcctat
     3361 acggataaca agcattatgc cgaaggccag gtggcttcct ttcaaaattt agtggataac
     3421 caattagtca tccgtaagga ttttgaaggc agggatatcg ttaccagcaa aaagtttgag
     3481 agtttcaaat ttgtatttga acggatcatc gccgaactcg atcccgggat ccgggtaatc
     3541 aatgccaccg agggaggact gcccattacc ggagccccca accggaagct tgatcaagtt
     3601 ttggcggagc attgccaaat ggaacgggag atcagtcaac ggatccgggt aatttacgaa
     3661 agaggcctcc aggatacgaa acggcgccgg atggacttca actcaatcgc actcaagctc
     3721 aagacgctgg ttaatcaggg aataaccggt atggaagggt taatcgggag attgcaggag
     3781 ctaaggagag taacgttttc ggaggcattc gaccgaaaca aattgttacc ggcctttcag
     3841 caaatcgcct ccgcctatga gatggtcgtc tccagacggg agtttgatat tctgttaaaa
     3901 agtttgcggg atagtaaact tctcgtgatc aagatgagga tggccgaggt aaatatggtg
     3961 gccacccgtg aggaatttga cggcattctc caaagcagcc tgctcttttt cagtgagacc
     4021 caaaaatact tggagtatat ccgggattgt atgaaagaca tagtacccgc ggcagtgaag
     4081 gaggagcaat cttcggtgtc gtttggactg aactccgaaa cttgccggtc atttgaggga
     4141 cggatcaagc gtggcgagac attgaatgag atccagcgga ctttggacag ggtaatttcc
     4201 tggggcagta atggggatga aatggctagg gcgctgtatc tgcgggggct tatttctatc
     4261 agaagcaatc agttgggaaa ggccgtaatt gatctggaac aggctgtagc cttggataag
     4321 agcttggcca aggcatattt tatgttattc cggctttctc aaaagggcaa agacttggtg
     4381 acggcctgta cctacctgga aaggtgccgt catttaaact atagaacaga gtattgctta
     4441 cgcaggcaaa ttaagaatta ttacctgatt aaggattatg ttgccgtcaa caatttcatc
     4501 accgaacatc gggagaaact gacaccacgg cggtttttca gtatcttact tggtgaatgt
     4561 ttgagttttt t
//
LOCUS       DASVMB010000580         4445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_70005,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000580 DASVMB010000000
VERSION     DASVMB010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70005"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1406)
                     /gene="miaB"
                     /locus_tag="VF531_16250"
     CDS             complement(75..1406)
                     /gene="miaB"
                     /locus_tag="VF531_16250"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7715572.1"
                     /translation="MMAQTYWITTFGCQMNVHDSEVLAGILESLGYKSAASVDEADLI
                     LLNTCCVRENAENRTYGHIGNLKSLKEQNPRRVIGVCGCMAQEPEEAAKLKATFPWVD
                     LVFGTHNLHQLPELLEQVRDGGNRVFEVWETDGAIYEGLPVKRDDPFKAWVTIMYGCN
                     NFCSYCIVPYVRGRERSREPERILDEIRGLASAGVREVTLLGQNVNSYGKDLSGGSWN
                     FARLLREVAQNTGIRRIRFQTSHPKDLSAELIEVMATEDKVCRHLHLPVQAGSSRILR
                     LMNRGYSKEEYEALVERIREKVKGLALTTDIIVGFPGETEADFRDTLDLVKKVRYDGA
                     FTFIYSPRIGTPAAKMEDPVPAPEKQKHLEELIAVQNQISLEKNQACLGTLQEILVEG
                     PSERNPEIWAGRSSQNKLVHFQPVPKLSQGDMVMVRITAAQTFTLEGEILK"
     gene            complement(1474..2394)
                     /gene="ftsY"
                     /locus_tag="VF531_16255"
     CDS             complement(1474..2394)
                     /gene="ftsY"
                     /locus_tag="VF531_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7715573.1"
                     /translation="MPDLNIFARLKAGLGKTKSNLVTNLEQIFTGSGSIDQDFYTDLE
                     DVLIMADVGMETSQFLLDRLRQVVKQENIKDPCKLKPVLIREITRMLEQVSARPFDFT
                     AKKRVYMLVGVNGVGKTTTIAKLAARFQAARSQVLLVAADTFRAAATEQLMLWGERLG
                     APVIHHQEGADPAAVVYDGMTAAKARGSDVVLVDTAGRLHTKVNLMEELKKVQRVVNT
                     NLDGRILETILVLDATTGQNAVSQTKIFHEALGLNGLILTKLDGTAKGGITLAIANQF
                     QIPIYLIGVGEKAEDLQTFDAASFTEALFE"
     gene            complement(2646..>4445)
                     /locus_tag="VF531_16260"
     CDS             complement(2646..>4445)
                     /locus_tag="VF531_16260"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7715574.1"
                     /translation="SENRIRQYQAIMEQYGCKHAVDLLEFDPEYRPVLNYLLNQTIVA
                     PDLKVAVQISSRLDKGFRIVTPEGDLVNPGGSITGGSVDKRRLGLLSRRKEIEDFKAE
                     KHNAEVALAKGERAVERLREELRGLFHELELTKKNENEAKLAKIAVERELVTIVQDQK
                     RAQDEIDGYRGQMAELQGEGERFDAGKEELTVAIGAEETKLQEIQQRLGELAQTSRSL
                     KQEKETIIQKITELKSLLSAGTQEQNGKVALKERLDRQVRENGETLKDIQAKQTQLAE
                     ERLRIREAQTGLAEKIKNDGTRLVEQEQRIAAGKEEKEALAVRIRELENRQRGFRRKN
                     SEYQNQLHHFELSISQKQLAMENVQNDLVEDYGAEWSAKLDPDWVKPEDIEELLESLK
                     TELKDLGPVNLAAIDDFMKLKERYEFLNNQSQDLIKAKESLLKVIGEIERTIIKRFTE
                     TFELVRVEFKKIFSELFEGGNSDLLLIDPENPLESGIEIIAQPPGKRLQSLSLLSGGE
                     RAMTAIALLFSILEIKPSPFCILDEIDATLDEANVHRFALLLELFSKKLQFIVITHRR
                     GTMETANALYGVTMEELGVSKLISLDLNEKKAG"
BASE COUNT          953 a         1309 c         1057 g         1126 t
ORIGIN      
        1 aaatgtagga attccttgga gggctccatc ggaaaaggag tgatcatccg gtcgtgggaa
       61 aaagtctcgc cgggttattt caagatctcc ccctccaagg tgaaggtttg ggctgcggta
      121 atccggacca ttaccatatc gccctgtgag agtttgggta caggttgaaa atggaccagt
      181 ttattctggg aggatcgacc cgcccagatc tccgggtttc tttcgctggg cccttccacc
      241 aggatctctt gcagagtgcc gaggcaagcc tgattcttct ccagcgagat ctggttctgg
      301 acagcgatca gttcctccag gtgcttttgt ttctcgggcg cagggaccgg atcctccatt
      361 ttggcagccg gagtcccgat ccggggcgaa taaatgaagg taaaagcccc gtcataacgg
      421 acctttttaa ccaaatcgag cgtatcccga aaatcggcct cggtctctcc gggaaacccg
      481 acaatgatat cggtagtcag cgccagcccc ttgacttttt ccctgattcg ttccaccagt
      541 gcctcatact cttccttgga atacccacgg ttcatcaaac gcaaaatccg gctgcttccg
      601 gcctgaaccg gcaagtggag gtggcggcag accttatcct cggttgccat gacctcaatc
      661 agctcggcgg ataaatcctt gggatgggag gtctgaaacc ggatcctccg gattccggtg
      721 ttttgggcta cttcccggag caagcgggcg aaattccagg atccgccgga gaggtccttg
      781 ccgtaggagt tgacattctg ccccaataac gttacttccc ttaccccggc actggcgagg
      841 ccgcggattt catccagaat gcgctccggc tcgcggcttc gctccctgcc gcgaacatag
      901 gggacaatgc aataggaaca gaaattatta caaccgtaca tgattgtgac ccaagccttg
      961 aaaggatcat cccgtttcac cggcagccct tcataaatcg cgccgtcggt ctcccaaacc
     1021 tcaaaaaccc ggttcccgcc gtcacggact tgctccagca actccggcag ttggtgcaga
     1081 ttatgggtac caaagaccag gtctacccag ggaaatgtcg ccttcaactt ggcggcttcc
     1141 tcgggttcct gggccatgca gccgcagaca ccgatcacac gacggggatt ctgttccttc
     1201 agggatttca ggttgccaat atgaccgtag gtgcggttct cggcgttttc acggacacag
     1261 caagtattga gcaaaatcag atccgcctca tccaccgaag cggccgattt atatcccaaa
     1321 gactcgagga ttccggccag aacctccgaa tcatgcacgt tcatttgaca accaaaagtg
     1381 gtaatccaat aggtttgtgc catcatttat ccccgaaatt catggtgcac atgtaggtac
     1441 gcgtgccatg gcacgtacct acatgtacct aaatcactca aacaacgcct cggtaaaaga
     1501 ggcggcatcg aatgtctgaa gatcctccgc cttttcacct accccgatca gatagatcgg
     1561 gatctgaaac tgattggcta tggccagggt gattccacct ttcgccgtac cgtccagttt
     1621 ggtgaggatg aggccgttga gtcccaaagc ctcatgaaag atcttggtct ggcttaccgc
     1681 attctgtccc gtggttgcat ccagtaccaa aatggtctcc agaatccggc catccaggtt
     1741 ggtgttcacc acccgttgta cctttttaag ctcctccatc agattcacct tggtatgtag
     1801 ccggccggcg gtatccacca gcacaacatc ggaaccgcgg gctttggctg cagtcatgcc
     1861 gtcataaacg accgccgcag gatcggctcc ttcctgatga tggatgaccg gagcccccaa
     1921 gcgttcaccc cagagcatca gttgctccgt ggcggcagcc cggaaggtat ccgccgctac
     1981 caataacact tgactacgag cggcctggaa ccgggcagcc agtttggcga tggtcgtagt
     2041 cttcccgaca ccgttcaccc caacaagcat ataaaccctt tttttggccg taaaatcgaa
     2101 cggcctggct gatacctgct ccaacatccg ggtgatctcc cggatcagca ccggcttcag
     2161 tttgcaggga tccttgatat tttcctgttt cacaacctgt cgtaaacgat ccaggagaaa
     2221 ctgactggtt tccatgccga catcggccat gatcagaaca tcttcaagat cggtataaaa
     2281 atcctgatcg attgaaccgg agccggtgaa tatctgctcc agattcgtga ccaggttgga
     2341 tttggtcttt cccaaaccgg ccttaagccg ggcgaatata ttgagatccg gcatagcaac
     2401 tctccttcat tccaacttgg tctcccgtcc gtggagcctg tcttcgttgc cgcaccccgc
     2461 ggggcgcgct acctatgcct cacgcctatt gcctgttccc tgttgcctat tcgcaactca
     2521 cccccgtccc ctctctttct gaaaagagag gggtgtaggg ttgtactgat agctctagac
     2581 cacgcggggt gagttccctc gcgcctcatg cctatttaca cctagtatct cgtacccctt
     2641 acgaattaac ccgccttctt ttcattcagg tcgagggaga tcagtttgga gacccccaat
     2701 tcttccatgg tgactccgta aagggcgtta gccgtctcca tggtaccgcg gcggtgggtg
     2761 atcacgataa actggagttt tttactgaaa agctccaaaa gcaatgcaaa ccggtggaca
     2821 ttagcctcgt ccaaagtggc gtcgatctcg tccaaaatac agaagggcga tggtttgatc
     2881 tcaaggatcg aaaaaagcag ggcgatggca gtcatggccc gctcgccgcc ggagagaagc
     2941 gacaggcttt ggagtctttt cccgggtggt tgggcaatga tctcgatccc cgattccagg
     3001 ggattctccg gatcgatcaa cagcaaatcg gaatttccgc cctcaaacaa ttcactgaag
     3061 atcttcttga attccacccg gaccagttcg aaggtctcgg tgaaccgctt gataatggtc
     3121 cgctcgatct cgccgatcac tttcaacaac gattctttgg ccttgattag atcctgggac
     3181 tggttgttta gaaattcata acgctctttg agcttcatga aatcgtcgat cgccgccaga
     3241 ttcaccggac ccaggtcttt taactcggtc ttcaacgact ccagcaattc ttcgatatcc
     3301 tccggcttaa cccaatccgg atcaagctta gccgaccatt ccgcgccgta atcctccacc
     3361 aggtcgttct gaacattttc catcgccagt tgtttctggc tgatggaaag ttcaaaatgg
     3421 tgcaactgat tctggtattc actgtttttt ctgcggaaac cgcgttgccg gttctccaat
     3481 tcccggatcc ggaccgccaa tgcttctttt tcctctttgc ctgcggctat ccgctgttcc
     3541 tgctccacca aacgggtccc gtcattctta atcttctcgg ccaaacccgt ttgcgcctcc
     3601 cggatccgta aacgttcctc ggccagttga gtctgtttcg cctgaatatc ctttaaggtc
     3661 tcaccgttct cccgaacctg tcgatccaaa cgctccttta acgcaacctt accgttctgt
     3721 tcctgtgttc cggcgcttaa tagggatttt agttcggtga tcttttgaat gatcgtctct
     3781 ttttcctgtt ttaggctacg cgaagtctga gccagttcgc caagacgctg ctgaatctcc
     3841 tggagcttcg tctcctctgc gccaatggcg acagttaatt cctctttgcc tgcgtcgaac
     3901 cgttccccct caccctgtag ctccgccatc tggccgcgat agccgtcaat ttcgtcctgg
     3961 gcgcgttttt gatcctgaac gatcgtgacc agctcacgtt cgaccgcaat cttggccagc
     4021 ttggcttcgt tctcattttt cttcgtcagt tccaattcat ggaagagtcc ccgtagttcc
     4081 tcccgcaacc gctccacggc gcgctctccc tttgccagtg cgacctcggc gttatgtttt
     4141 tccgccttaa aatcctcgat ttccttgcgc cggctcaata atcccaaacg ccgcttatca
     4201 acgctaccgc cggtgataga accacccggg ttgaccaagt ctccctcagg ggtgacaatt
     4261 ctaaacccct tatccaaccg ggaactgatc tggactgcca ctttcaagtc aggcgcaaca
     4321 atcgtttggt tcagcagata attaaggacc ggacggtact ccgggtcaaa ctcaagcaga
     4381 tcgacagcat gtttacagcc atattgctcc ataatggctt gatactgccg gattcggttt
     4441 tccga
//
LOCUS       DASVMB010000581         5316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_586667,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000581 DASVMB010000000
VERSION     DASVMB010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586667"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VF531_16265"
     CDS             <1..1341
                     /locus_tag="VF531_16265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7715575.1"
                     /translation="PVPRAQESQKLPTMLAPGDAPDVCFSYDKALYGLYVKNGALLDY
                     TNLINRYGKNIKKQFSADDLALGKMNGRIYSIVYQSVSTADTTWIRKDWLDKLGMKEP
                     TNPTEFYNMLKAFKENDPGKVGDNLIPFAFSGSNLYPFGVWYGTVLGGFLKDPPSKNQ
                     LFEQVYPMWPQTKDCMKFLNKLYNEKLLTDRYLLDKDAVLFQQDFSRGVIGSMVHFAH
                     WPYHSAFSNMYENLQKNVPEAQLVSTFPWKHEQSKENYIELIRNAPFGYMWFSPKTAK
                     HPDLVMKYLDWLAGDEACWTAWFGQKGVDYQLNDGIPVPIDDAKYKKRVPWIGSQYNT
                     LRNPFIQQPQQYIKRQAMDFNAKYRQQFIKETVAGNKAIKYYQPWLTVPTPVSDKLTA
                     SLARSWEYLEVKIITAPADQFDTVFDDAIKQYRQAGGDEVAKELAAAYKTQYGK"
     gene            1494..3914
                     /locus_tag="VF531_16270"
     CDS             1494..3914
                     /locus_tag="VF531_16270"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7715576.1"
                     /translation="MLQSTSAKIKKWLIRYKGTGKFFAYFIAGSMLVTGIVGSLIYLG
                     SARALELEAIDANQNMLIQLNKSTDLLLSQVDQYLSQLALDATVADFMQYYRSKNLTA
                     QMRINTAISNNLLLMNPYINSCTIFYFKENKVYSVNRGVRNLADFPDRDIFLEAKSGK
                     YLYNRNCFPTRRIVDEKTGKTINVITVVKPIPLDSSEAIGVAVVNIDESYLRDIMNSI
                     IAGDEQKIFVVDDQTRFISSNQFLQPHTNLRDKPYLNSAFRQKAGNYLAQADHQKVLI
                     SFVTSEAYNWKYVSIIPYRVIVKRIGFIRNYAFIISFFAIILGIAVSLFFSNKISRPI
                     NLIAGLFKDDKNPSDENDVLKYIENNIHRLMAKNESIEKTFLEHLPVLRNNFLTSFLM
                     GAVTHSELEERFDYYGIDLSGSSRYLVYLVSLDSSRGLTGKVSERQLNMLIIYLMEVL
                     NDLPNHDHKKIVVNTKANEIAVVFAFAGKDEESLNRELEMIGTEIQQAVQANANYTFA
                     IAAGNLKREIGQISDSYNEALEAIVYKVLIGNQKVIRYEDIQNLKAENFEYPYEKEKE
                     LMDAMKLGDQESVKRISHEIFEFFVNCSESLGDSIYYYYLQLLSSTIRRALRIGINIE
                     SIMGEANLYKELLKCNGIGEIQDWFTFLFRELTEHAGQRKNTKNRSIVESIVAYIQEN
                     YDQDLSLTALSQQVFLSVPYLSTIFREEYGKPLKQFINETRIEKAKEFLAEPGYQIAE
                     VAEKVGYDKVHAFLRLFKEYTGMTPGQYRKTIIFTQRKTPANIVHFKRHSSPSATGEV
                     HYAGKNKA"
     gene            3892..>5316
                     /locus_tag="VF531_16275"
     CDS             3892..>5316
                     /locus_tag="VF531_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715577.1"
                     /translation="MPEKIKLEFYQPSAEFSPMPFWFWNDALDESEIIRQIHDFRSKG
                     VMGFVIHPRIGIPDDLGYFDDRFMGYVKLAVSEAARNGMKVILYDEGMYPSGSAHGEV
                     VAGNPEFATGGLRMIEYSCSGVMEIHPPLQAGERVIATLAVEKSEACCIVPGSERQLS
                     DTGTCVHFETPTTGRWTVLFLVETFSGGTIRGIHFGEDDGEPGAPPSADLLNPAAVRK
                     FIRLTHDRYYEVLRDYFGTTVIAMFTDEPDLLGRGARPGVKPWTKGFLDWYRSRGNTA
                     EDLAALWFDTGDANGEKRKHYEQAVNERLEFSYYSQISKWCEDHGIALTGHPQASDDI
                     GSLKHFHIPGQDIVWRWVAPEGELGVEGPHSTMAKCSSDAARHDGRRRNANECFGCCG
                     PRGNHWAFSMDDMKWYLDWLFVRGVNLVYPHAFFYSIDGPKRSGERPPDVGPHNIWWP
                     YYRDISDYIKRMSWLMTDSVNLTGI"
BASE COUNT         1409 a         1249 c         1337 g         1321 t
ORIGIN      
        1 ccggtaccgc gggcccagga aagccaaaaa ctcccaacca tgctggctcc gggggatgct
       61 cccgatgttt gtttcagtta cgacaaagcg ctatacggtt tgtatgtcaa aaacggagca
      121 ttactggatt acaccaatct catcaatcga tacggcaaga atattaaaaa gcagttttcc
      181 gccgatgatc tcgcactcgg caagatgaac ggcagaattt attccatcgt ataccaatcg
      241 gtttctacgg ccgataccac ctggatccgt aaagactggc ttgataaact gggtatgaag
      301 gaaccgacca atcccacgga attttacaat atgctcaaag ccttcaagga gaatgatccg
      361 gggaaggttg gcgataatct gatcccgttt gcgttttcgg gttcaaacct gtatccgttt
      421 ggagtatggt atggcacggt gttgggggga ttcctgaaag atccgccgtc caaaaaccag
      481 cttttcgaac aggtatatcc gatgtggccc cagacgaaag attgcatgaa gtttttaaac
      541 aagctctata acgagaaact gttgaccgac cgctatttgc ttgataagga cgcagtcctt
      601 ttccagcagg acttctccag gggagtcatc gggtcaatgg tccatttcgc ccattggcct
      661 tatcatagcg cctttagcaa catgtatgaa aatctgcaga aaaacgtacc ggaggctcaa
      721 ttagtctcga ccttcccctg gaagcacgaa cagagcaagg agaactatat tgaactgata
      781 cgtaatgcgc cgttcggtta catgtggttc tcgccaaaga ccgccaaaca tcccgatctg
      841 gtcatgaaat acctggactg gctggccggg gatgaggctt gctggacggc gtggttcggg
      901 cagaagggcg tcgattacca gttaaacgac gggatcccgg tgccgattga tgatgccaag
      961 tacaaaaaac gggtgccgtg gattggatcc cagtacaata ccttaagaaa tccgttcatc
     1021 caacaaccac agcagtatat caaacgccag gccatggatt ttaacgccaa atatcgccag
     1081 caattcatca aggagacggt cgccgggaat aaagcgatta agtattatca accctggctg
     1141 accgtaccga ctccggtttc tgataaatta acagcatcct tagcccgatc ctgggaatac
     1201 ttggaggtca agatcatcac tgcgccggcc gatcagtttg acacggtttt cgatgatgcg
     1261 atcaagcaat accgccaagc cggcggcgat gaggtggcca aagaactggc cgccgcctat
     1321 aagacccagt acggtaagta agaatcacta aaagaatgca atcagcccgg tttttaaaga
     1381 gccgggctga ttggattatt ctttgctctg tttttagaaa tggttgattt cggtactaag
     1441 gaccggtgca gcaggtgtca agaatgacgc gagcggttgg tggagggaga tcaatgcttc
     1501 agtctacttc ggctaaaata aaaaaatggc tcattcgata caaaggcacg ggaaaattct
     1561 tcgcctattt tatcgccggt agcatgctgg tgaccgggat tgtcgggtca ttgatatatt
     1621 tgggttccgc ccgggctttg gaactggagg cgattgacgc caatcagaat atgttgattc
     1681 aattgaataa atccaccgat ctcctgttat cccaggttga tcagtatttg agccagttgg
     1741 cccttgacgc cacggtggcg gattttatgc aatactatcg gtctaagaat ctgactgcgc
     1801 agatgcggat caatacggca atttccaata acttactgct gatgaatcct tatatcaatt
     1861 cgtgcaccat tttttatttc aaagaaaaca aggtttattc cgttaatcgc ggagtacgaa
     1921 atctggcgga ttttccggac cgggatatct ttcttgaggc gaaaagcggg aaatatcttt
     1981 ataatcgtaa ctgctttccc acacgccgga ttgttgatga gaaaacaggc aagaccatca
     2041 atgtcatcac agtggtcaag ccgataccgc tcgacagttc ggaagcgatc ggggttgccg
     2101 tggtaaatat cgacgagagc tatcttcggg atatcatgaa ctccatcatc gccggagatg
     2161 aacagaagat ctttgtggtt gatgaccaaa cccggtttat ttcaagcaac caattcttgc
     2221 aaccccatac caatctgcgc gataagccgt atctaaacag cgccttccgg caaaaggccg
     2281 gaaactatct agcccaagcc gatcatcaaa aggtcttaat ctcctttgtc acctcggagg
     2341 catacaattg gaagtatgtc agcatcattc cttaccgtgt catcgtgaaa cggattggtt
     2401 ttattcgcaa ttacgccttc atcatctcct tttttgccat tattctcggg atcgcagtct
     2461 ccttattctt ttccaataaa atttcgaggc ccatcaatct tatcgccggg ctttttaagg
     2521 atgataaaaa tccatcggat gagaatgatg tcctgaaata cattgaaaat aacattcatc
     2581 gcctgatggc gaagaatgaa agcatagaaa agacttttct cgaacatctg ccggtgttgc
     2641 gcaataattt tctcaccagt tttctgatgg gggccgtgac ccattccgag cttgaagaga
     2701 gatttgatta ttacggcatc gatctctccg gcagttcccg ctatcttgtt tacctggtgt
     2761 cactcgacag ttcccgcgga cttaccggaa aggtatcgga acgccagctg aatatgttga
     2821 tcatctattt gatggaggtt ttaaatgatc tgccgaacca cgatcataaa aagattgtgg
     2881 taaataccaa ggccaacgag atcgcggtgg tatttgcctt tgccggtaaa gatgaggaat
     2941 ctttgaaccg tgaacttgaa atgattggaa ccgaaattca gcaagcggtt caggccaatg
     3001 ccaattacac ctttgccatt gcagccggaa atctgaaacg agagatcggc cagatatccg
     3061 attcatacaa cgaggccctg gaggccatcg tttataaggt acttatcggt aatcaaaaag
     3121 tgatccgcta tgaagatatt caaaatttga aagccgagaa ttttgagtac ccctacgaaa
     3181 aagagaaaga attaatggat gcgatgaaac tgggtgatca ggagtcagtc aagcggatca
     3241 gccacgaaat atttgagttc ttcgtgaact gcagcgagtc attgggtgat tcgatttact
     3301 actattacct gcaactgttg agctctacca ttcgtcgagc tttgcgaatc gggatcaata
     3361 tcgaatcgat tatgggcgaa gccaatcttt ataaagagtt gttgaaatgt aacggaatcg
     3421 gtgagatcca ggattggttc accttcctct tccgggaact caccgaacat gccggacagc
     3481 gaaaaaacac caaaaaccgg agtattgtcg agtcaatcgt cgcatatatt caagaaaatt
     3541 acgatcagga tctgagcttg acggcattat cccaacaagt cttcctaagt gtgccttatc
     3601 tgagcaccat tttccgggaa gagtacggca agccgcttaa gcaattcatt aatgaaacgc
     3661 ggatcgaaaa agccaaggag tttctggccg agcccggtta ccaaattgcc gaagtggccg
     3721 aaaaagtcgg ctatgacaag gtacacgcct ttttaaggct tttcaaggag tataccggca
     3781 tgacacccgg ccaataccgg aaaaccatca ttttcactca aaggaagacg cctgcaaata
     3841 tcgttcattt caaacgccat tcatcgcctt ctgccacagg agaggttcat tatgccggaa
     3901 aaaataaagc ttgaatttta ccagcccagt gcggaattta gccccatgcc gttctggttt
     3961 tggaacgatg cgctggatga gtcggaaatc atcagacaga tccatgattt ccgctccaaa
     4021 ggagttatgg gatttgtaat ccacccccgg atcggcattc cggatgatct gggttatttc
     4081 gacgatcgtt tcatgggtta tgtcaaattg gctgtctccg aagccgctcg caacggaatg
     4141 aaggttattc tatatgatga gggaatgtat ccttccgggt cggcccacgg tgaagtggtt
     4201 gccggaaatc ccgagtttgc cacgggcggt ctgcggatga tcgaatattc atgctcggga
     4261 gtgatggaga ttcatccacc gctgcaagcg ggggagaggg tcatcgctac gctggcggtc
     4321 gagaaaagtg aggcctgctg catcgttccc ggtagtgagc ggcaattatc tgatacggga
     4381 acgtgcgtcc actttgaaac ccctactacc ggaagatgga cggtcctatt tttggtggag
     4441 acctttagcg gcggaacgat tcgcggcatc cacttcggcg aggatgacgg cgagcctggg
     4501 gccccgccat ccgccgatct gctcaatccc gcggcggtcc ggaaatttat ccgtcttacc
     4561 catgaccgtt actatgaagt gctccgggat tacttcggta ccacagtgat cgccatgttc
     4621 accgatgaac cggacctttt gggccggggc gcccggccgg gcgtaaaacc gtggaccaag
     4681 ggttttctgg actggtaccg gagccgcggt aatactgcgg aggaccttgc cgcattatgg
     4741 tttgataccg gcgatgctaa cggggagaag cgtaaacact acgaacaagc ggtgaatgaa
     4801 cggcttgaat tctcgtatta cagccagata tccaaatggt gcgaggatca cggaatcgcg
     4861 ctgaccggac atccccaggc gagtgatgat atcggatcgc tgaagcattt tcacatcccc
     4921 ggtcaggata tcgtctggcg ctgggtggcc cctgaagggg aattgggagt ggagggtccc
     4981 cacagcacca tggccaaatg ctcgtccgac gcggccagac atgacggaag acgccgcaat
     5041 gccaacgagt gtttcggttg ttgcggtccc cgggggaatc attgggcgtt ttccatggat
     5101 gacatgaaat ggtatctcga ctggctcttt gtgaggggag tcaatctggt ttacccccat
     5161 gctttctttt actcaatcga tggccccaaa cggagcggcg aaaggcctcc cgatgtcggg
     5221 cctcataata tctggtggcc ttattaccgc gacatttccg attatatcaa acggatgagt
     5281 tggttgatga cggatagtgt caatcttacc gggatt
//
LOCUS       DASVMB010000582         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_441125,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000582 DASVMB010000000
VERSION     DASVMB010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441125"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VF531_16280"
     CDS             <1..672
                     /locus_tag="VF531_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7715578.1"
                     /translation="RWQVIDGFLTSQPSRIYRTIMNLYHQGTLFQHIGITCLETVLGF
                     LLGTIFGTLIATILWWSDFLAKVCEPYLVVLNSLPKIALAPIFIVWIGAGPSAIIVMA
                     LAISLIVTILEVLNGFLAVDQDEVKLVQTFGATKLQIFTKVILPASFHSIINALKINV
                     GLSWVGVIVGEFLVSKAGIGYLIVYGGQVFQLDLVMASVIILAIAAAAMYQAVVYLEK
                     FLVKW"
     gene            764..1699
                     /locus_tag="VF531_16285"
     CDS             764..1699
                     /locus_tag="VF531_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7715579.1"
                     /translation="MAKESLVKVRVNEVTHSVFYAPQYAAINLGIFKAEGLDVELTNG
                     AGTDKVMTAVLSGQADVGFGGPEATLYVYNEGKADYPVVFAQVTNRDGSFLVGRKPEP
                     KFTWENVKGKKIIGGRTGGMPEMTLEFVLKKKGLVPGKDVDVDTSIQFALMAPAFLNG
                     QGDYVALFEPTASMLEKEGKGYVVASIGKDSGEVSYTTYFAKQSYLKKNAGTVQKFTN
                     ALYKGQLWVAKHTPQEIAKAIAPSFPDTREEILTLVAARYKDQDTWNTDPVLKKKSFE
                     LMQAIMKEAGELKNPVPYEKITDPSFAEKAMKSVK"
     gene            2076..2447
                     /locus_tag="VF531_16290"
     CDS             2076..2447
                     /locus_tag="VF531_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715580.1"
                     /translation="MKTRLVVIFLIFTLVISGMVLAADYKCIGDRIEKSGSTWGYAKI
                     SGSDYRIEKGSSTIAFVKSRSGKWAIEDAGNNTLGWLNGSSIETTNGSTWASLSDAKS
                     FCDGPDPVAAAMWVLNKTGKL"
     gene            2690..>3474
                     /locus_tag="VF531_16295"
     CDS             2690..>3474
                     /locus_tag="VF531_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX7715581.1"
                     /translation="MRRTSLILLLLAVLVIAVPIHAEVAASAPRIVAVAAENPDMYNL
                     YTMEIDGKNPVKIYTADSLILTIYWLSPDGGTVYFQSDEGIFCVGLDGKNLRPAEFQG
                     YHPQLSPDGKRVVFARLSFSMDSSSLYLMNLDGTSLNGLTEEKKEIMDEDPSWSPDGK
                     RIVFTRNHKLWILDLDSKEQQEVKISDDDLDCYSPRWSKDGSKIAFIGESGINQDIYL
                     ADLQTGETTAITDDGSLKRSLLWTPDGTKLAYSFGNSFEEEKS"
BASE COUNT          952 a          711 c          885 g          926 t
ORIGIN      
        1 agatggcaag tgatcgatgg ttttttaacc agccaacctt ccaggattta tcggacgatt
       61 atgaatctgt atcaccaggg aaccctgttt caacatatcg gtattacatg tctggaaaca
      121 gtcctgggtt ttttgcttgg gaccatattt ggaacgctca ttgccacaat cttgtggtgg
      181 tcggattttt tggcaaaagt ctgtgaaccg tatttggtgg ttctaaatag ccttcccaag
      241 attgcgttgg cgccgatctt tatcgtatgg atcggtgcgg ggccgagcgc gattattgtc
      301 atggcgttgg cgatatcgtt gattgtaacc attttggagg tcctgaacgg ttttttggcg
      361 gttgatcagg atgaagtcaa attggttcag acgtttggcg ctaccaaact gcagatcttc
      421 accaaagtca ttttacctgc ctcttttcac agcatcatca atgccttaaa aatcaatgtc
      481 ggtttgtcgt gggtcggtgt gatcgtcggt gagtttttgg tttccaaggc ggggatcggt
      541 tatctgatcg tatacggagg tcaggttttt caacttgatc tggtgatggc cagcgtcatc
      601 atcctggcga tcgccgcggc agccatgtac caggcagtgg tttatctgga gaagtttttg
      661 gtgaagtggt gatgaaaggt aaagagcata aaggaggatg gtcgtgaaaa ggtattttct
      721 gatggcggcg ttgattcttt gtctttgtct atccattccg ggcatggcaa aagaaagtct
      781 ggtcaaagtc agggtcaacg aggtaaccca ttccgtgttt tatgctccgc aatatgcggc
      841 gatcaatttg ggtatcttca aggcagaggg tttggatgtg gagctaacca acggggcggg
      901 cacggacaaa gtaatgacgg cagtgctttc gggccaggca gatgtcgggt tcgggggtcc
      961 ggaggcgacg ttgtatgttt acaacgaggg aaaagccgat tatccggtag tctttgcaca
     1021 ggtcaccaac agggatgggt catttttggt cggacgcaaa ccggagccca aattcacgtg
     1081 ggaaaatgta aaaggtaaga aaataatcgg tggacgcacg ggcggcatgc ctgaaatgac
     1141 cctagagttt gtattgaaga aaaagggtct ggtgccaggg aaagatgtcg atgtagacac
     1201 cagcattcaa tttgccttaa tggcaccggc ttttttaaac ggccagggag attacgtcgc
     1261 tttgtttgaa ccgactgctt ccatgcttga aaaagagggc aaggggtacg tggtcgcatc
     1321 catcggcaaa gacagcggcg aggtatctta taccacatat ttcgcgaaac agagttatct
     1381 caaaaagaat gccggaaccg tgcagaaatt caccaatgcc ctttataagg ggcagctatg
     1441 ggtggcaaag cacacgcccc aggagatagc caaggccatc gccccgtcgt tccccgatac
     1501 ccgtgaggaa atattgactt tggtcgcagc gcgctataag gatcaggata cctggaatac
     1561 ggatccggtt ttaaaaaaga agtcttttga actgatgcag gcgatcatga aagaagcggg
     1621 ggaactcaag aatcccgtgc catatgaaaa gatcacggat ccttcttttg cggagaaagc
     1681 gatgaagtcg gtaaaataat ataaacaaac gaaaacggta ccacgaaccc ggtttatacc
     1741 gggaatcatc tctaaatact aaacaaacgt cccaagacgt ttgttttttt acttaaataa
     1801 ataaattacc acaatttata gattactgca ggatttctta taattttcac gaacataata
     1861 aaataatgga tattttcatc tatttctttt caaagctgcc tgccactttg gatacgattg
     1921 aagatgctct ggatgagctt ttagagggcg aaggcgaagt caccggtggc ggtactggta
     1981 agtctgaccg gcccactaga atttgatgat atcccaatca tccgattctt tatcataaat
     2041 ggagtgggaa ataaaacaag gaggaattga tcgtcatgaa gacccgtttg gtagtgattt
     2101 ttctgatctt taccttggtt atatccggaa tggtcttggc agccgactat aagtgtatcg
     2161 gcgaccggat cgaaaagtcc ggcagcacct gggggtatgc caaaatctcc ggttccgatt
     2221 accggatcga gaaaggcagc tccaccattg cgtttgtaaa aagccgcagc ggtaaatggg
     2281 cgatcgagga tgccggaaac aacaccctgg gatggcttaa cggcagttcg atcgagacca
     2341 ccaacggcag tacctgggct agccttagtg atgccaagtc gttttgcgac ggtcccgatc
     2401 cggtagcggc cgcgatgtgg gtgcttaata agaccgggaa gctttaagac attacccaat
     2461 tgctctaatg cgaaagaggt aagtgggtat tccatttacc tctttactgt tgatcaattc
     2521 taatcatccc gttttatctt aagttactcc caatccaata atttattata tattttataa
     2581 aaatgattcc cggattaaac tcagaataca taaaaatttg aactaaattc cccatgttcg
     2641 ataagggaaa cacacattat caatgctaaa aatgaaaggg aagatttaaa tgagaagaac
     2701 aagcttgatt ttattacttt tggcggttct ggttattgcc gttccaattc atgcggaagt
     2761 agcggcatct gcgccgagaa ttgtggctgt cgcagccgag aatccggata tgtataacct
     2821 ttatacgatg gagatcgacg ggaaaaaccc ggtaaagatt tatacagcgg attccttaat
     2881 tcttaccata tattggcttt cgccggatgg cgggacggtt tatttccaaa gcgatgaagg
     2941 gattttctgc gtcgggcttg atggtaaaaa ccttcgtccg gccgaatttc aaggatatca
     3001 tccccaacta tctccggatg ggaaaagggt ggtatttgcc aggttaagct tttccatgga
     3061 ttcaagcagc ctttacctga tgaacctgga tgggacctct ctgaacggac ttacggagga
     3121 gaaaaaggag attatggacg aagacccatc atggtcgccc gatggaaaac ggatcgtttt
     3181 tacccggaat cataaattat ggatcttgga cctggattca aaggaacaac aagaagtaaa
     3241 gatttccgat gacgatctgg attgttactc tcctcgctgg tcgaaggatg gttcaaaaat
     3301 tgcttttatc ggtgaatctg gaatcaatca ggacatttat ctggcagacc tccagaccgg
     3361 ggaaacaacg gcaattaccg acgacggatc cctcaaacga tctttattat ggacccctga
     3421 cggaacgaaa ctggcctatt ccttcggcaa ctcctttgag gaggaaaagt ccaa
//
LOCUS       DASVMB010000583         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_70466,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000583 DASVMB010000000
VERSION     DASVMB010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70466"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF531_16300"
     CDS             complement(<1..225)
                     /locus_tag="VF531_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715582.1"
                     /translation="MAKTENKRTIKGNTGTEKYEIRAAICFLAPSLIGFGIFFLLPFI
                     GGVYYSLVDGPIDGHFVGIENYRSLLTNSIF"
     gene            504..1199
                     /locus_tag="VF531_16305"
     CDS             504..1199
                     /locus_tag="VF531_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7715583.1"
                     /translation="MSGGRVLVVDDETEIRELIVKYLRREGIQTEEAAGGITAVAQIM
                     QQDFDLVILDIMMDDLDGFEVLKQVRPVKPKIPMLLLSARQEDYDKVLGFGLGADDYV
                     TKPFSPAELTARVKAHIRRTRTLYTQLNDENATLERGPLRLDTKSYTLTKNNVPIELS
                     ARELRLLRFFMENPGQVFTKTQIYRNAWENEYYDDNSVMVYISRLREKIEDNPREPVF
                     IQTVWGIGYKFGA"
     gene            1263..>1977
                     /locus_tag="VF531_16310"
     CDS             1263..>1977
                     /locus_tag="VF531_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715584.1"
                     /translation="MQLRFKIVIVFILSLIVSVIVFLAIFAVLIATPFSGGYGEKDMN
                     LAANAITGAIASLPEINKQILAPIITKWQKKYPNMAFSVASDSKVLFSTAPQNDRTTA
                     NLLMKIAKFPMAFPNELPAADLEGLDGKLPEGLKLLGQVIPKTGQPTARSRQQVAIVK
                     SPKSLGPVNQIFISNYVQSATPPKKNSLILYTYQQRSPEERVLAREIKHSGKTIGLLL
                     ITVKGQFYTAIELFTDPQKA"
BASE COUNT          550 a          454 c          461 g          512 t
ORIGIN      
        1 aaatatggaa ttcgttagca aggatcgata attctcaatc ccgacgaagt gtccgtcaat
       61 tggaccgtcg actagtgagt aataaactcc cccgatgaag ggaagtaaaa agaagatccc
      121 aaaaccaatc aggctcggtg ccagaaaaca gattgccgcc ctgatctcat atttttcggt
      181 tcccgtgttc cctttgatgg ttctcttatt ttccgtttta gccatgatga ttaggttttc
      241 cgctccattt gaaatagtga gctcgtttgc aaggttgata gtgataattt tagttatcgc
      301 ttttaaaatg gtacttaatc aattcttaaa agactcttac agtttcgctc cctatagttc
      361 cggttccgat agcgtggttg taagattttt tctagaaatc attaagcttt gtgtaattct
      421 actttgataa aatgaagcta aatccatata gcagagagtc tgtacgcact ccagcgcaaa
      481 agtaacctta tcggaggttt cccatgtccg gtggcagagt tttagtagtt gacgatgaaa
      541 cggagatccg ggagttaatc gttaaatact tgcgacggga aggaatacaa actgaagagg
      601 cagcaggggg tatcacggct gtggcgcaaa ttatgcaaca ggactttgat ctggtgatac
      661 ttgatatcat gatggacgat ttggatgggt ttgaagtatt gaaacaagtc aggccggtaa
      721 agcccaagat cccaatgctg ctgctcagcg ccaggcagga agattacgat aaggtgcttg
      781 gtttcgggct cggtgccgac gattacgtca ccaaaccttt cagtccggcc gaattgacgg
      841 cacgggtcaa ggcgcatatc cggcgcaccc gtaccctgta cacccagctg aatgatgaaa
      901 acgccaccct tgaaagaggg ccattgcggc tggatacaaa atcgtatact ctgaccaaaa
      961 acaatgtccc tattgagctc tccgccagag agcttcgatt gttgaggttt tttatggaaa
     1021 accccggtca ggtctttacc aaaactcaaa tctaccgcaa cgcatgggaa aatgagtatt
     1081 acgacgataa ttcggtgatg gtctatatca gccgtctgcg tgaaaagatc gaggataacc
     1141 ccagagaacc ggtgtttatc caaaccgtct ggggcattgg gtataaattt ggggcttaat
     1201 atgctttgga cacgggaaat tacacgaact tacgcgtacg catttgatac tgaggaggat
     1261 ttatgcaatt acggtttaag attgtgatcg tcttcatctt atccctgatt gtatcggtta
     1321 tcgtcttttt agcgatcttt gccgttttga tcgccacccc ttttagcggc ggatacggtg
     1381 aaaaggacat gaacctagcg gccaatgcaa tcaccggcgc catcgcttcc ctccccgaaa
     1441 tcaacaagca aattttggcg ccgatcatca ccaaatggca gaaaaaatat ccgaatatgg
     1501 ccttttcggt tgccagcgat tccaaagtgt tattttccac cgccccccaa aatgacagaa
     1561 ccaccgcgaa cctgctaatg aagatcgcca agtttccgat ggctttccca aatgagctgc
     1621 ctgcggcaga tctcgaaggt ttggatggaa aattgccgga gggactgaaa ctgctcgggc
     1681 aggttattcc caaaaccgga cagccaaccg ccaggtcccg gcaacaggta gcgatagtga
     1741 aatcgcccaa atcattaggc ccggttaatc agatattcat cagcaattac gttcaatccg
     1801 caactccgcc gaaaaagaac agtctaatcc tctatactta tcagcaacgg agtccggaag
     1861 agcgggtctt agcccgggag attaagcatt ccggtaaaac gataggttta ttgctgatca
     1921 ccgtcaaagg tcaattctat accgcgattg aactgtttac cgatccccaa aaagcgg
//
LOCUS       DASVMB010000584         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_441364,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000584 DASVMB010000000
VERSION     DASVMB010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441364"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VF531_16315"
     CDS             complement(<1..1463)
                     /locus_tag="VF531_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX7715585.1"
                     /translation="MNDLFRADGNRLIREFDAEKLWVEPWGENSLRIRATYMPTVLDD
                     YWALLPQKSRNGKISIVPHQASIENGKIRLEIDETGRITICNQHGKVLLKEYARDWDS
                     RETPASLKIRARQFKPVPGGDYQLTVRFESNPDEKIFGMGQYQHPYLDLKNCTLELAH
                     RNSQSSVPFALSNLGYGLLWNNPAFGRVTFGKNMTEWVAESTKQLDLWVTAGDTPAEI
                     EEAYAGATGTVPMMPEYVMGFWQCKLRYQTQAELLEVAREYKRRGLPLSVIVVDFFHW
                     TKQGEWQFDPDYWPDPDAMVAELKEMGIELMVSIWPTVDKTSENYQEMLQKGYLTRVD
                     RGIRTTMQFFGDTVFYDATNPAARDYVWQVIKKNYYDKKIRIFWLDEAEPEYSVYDFD
                     NYRYYLGPVLQVGNVYPVMYAKTFYDGMAQAGQQNIINLIRTAWAGSQRYAALAWSGD
                     IQSSFNTLRNQVKAGLNMALAGIPWWTTDIGGFFGGN"
     gene            complement(1593..>2187)
                     /locus_tag="VF531_16320"
     CDS             complement(1593..>2187)
                     /locus_tag="VF531_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715586.1"
                     /translation="TKPLFPANPMATKMGTNVFEMPKSNKHPERTMLWINEFYGEEMS
                     AQGYYGSFGIGMKKNQNGTYTQLPPAESGYSQDRWMWTNAFVDGAPMYGSKELEKRVT
                     IVPELMSRIEYDDIYQPYFPKAKDIYPMVKFKKSAVDELSLINADITKLVDQKKAEWI
                     VKGGIEKDWKTYLEQLNKIGLPRVKQIYQEAYNQYYK"
BASE COUNT          550 a          565 c          439 g          633 t
ORIGIN      
        1 atgttccctc cgaaaaatcc cccgatatcc gtcgtccacc atgggatacc ggctaatgcc
       61 atatttaaac cggccttaac ttgatttcgt aaggtgttga aactcgattg aatatctccg
      121 gaccaagcta gcgccgcgta acgttgactt ccggcccagg ccgttcgaat caggttgatg
      181 atattttgct gtcctgcctg agccataccg tcataaaacg ttttggcata catcacagga
      241 tagacatttc ccacttgtag aacaggacca aggtaatagc ggtaattgtc aaaatcgtaa
      301 acgctatatt ccggttccgc ttcatccagc caaaaaatcc gaatcttctt atcataatag
      361 ttctttttta taacttgcca tacataatcc cttgcggccg gattggtggc gtcataaaaa
      421 accgtatcgc caaaaaactg catggtggtt cgaatacctc tatccacccg agtaagatat
      481 cctttttgca gcatctcctg ataattctca ctggtcttgt cgacagttgg ccagatcgag
      541 accatcagtt caatacccat ctctttcagc tcagcaacca tggcatcggg atccggccaa
      601 taatccggat cgaattgcca ttctccctgc ttggtccagt gaaaaaaatc gactacaatc
      661 acggaaagcg gaagcccccg gcgtttatat tcccgggcga cctccaacaa ttcagcctgg
      721 gtttgatagc gtaacttaca ttgccaaaac cccataacgt actcgggcat catcggtacg
      781 gttccggtag ccccggcata ggcctcttcg atctctgccg gtgtatctcc ggctgtgacc
      841 caaagatcaa gttgcttggt ggattcggcg acccattcgg tcatattctt gccgaatgtg
      901 acccgaccaa aggccggatt gttccatagt aaaccatacc ccagattgga cagggcaaag
      961 ggaacgctgg attgagaatt acggtgggcc aactccaatg tgcagttctt taggtcgaga
     1021 tagggatgct ggtactgtcc cataccgaag atcttttcat ccggatttga ttcaaaacga
     1081 acagtcagtt ggtagtctcc acccggtacc ggcttaaatt ggcgggcacg aatcttcaat
     1141 gaagcgggag tttccctact atcccaatct cgcgcatact ccttgagcag taccttgccg
     1201 tgttggttac aaatcgttat tctcccggtc tcatcaatct ccaggcggat tttgccattc
     1261 tcaatggatg cttgatgagg aacgattgaa atttttccat tccttgattt ctgcggcaat
     1321 agcgcccaat agtcatcgag tactgtcggc atatatgtgg cgcgaatccg aaggctattt
     1381 tcaccccatg gctccaccca gagtttctct gcgtcaaatt cccggatcaa cctattcccg
     1441 tctgcccgaa atagatcatt cactcataat cctccttgca atccgttaac ggattatcaa
     1501 agttaatgca gggagaattt ccattctccc tgttcaaaac ctataaaaaa cgcgcaagaa
     1561 ccattgcaac aaatcttagt tgggttttct aattatttat aatactggtt atacgcttcc
     1621 tgatagatct gttttactct gggcagaccg attttattta attgctccaa ataggtcttc
     1681 cagtcttttt caataccgcc tttgacgatc cattccgctt tcttctggtc caccagtttc
     1741 gtgatatccg cattgattaa ggacaattca tcgactgcgc tctttttgaa cttgaccatc
     1801 ggataaatat cttttgcctt tggaaaataa ggttgataga tatcgtcata ttcgattcga
     1861 ctcatcaatt cagggacaat ggtaacccgc ttttccaatt ctttggatcc gtacattggc
     1921 gcgccatcaa caaaagcgtt tgtccacatc caacgatctt ggctgtatcc gctttctgcc
     1981 ggaggtaact gggtataggt gccattttga ttcttcttca tcccgatacc gaaagagccg
     2041 taatatccct gggcagacat ctcttctcca taaaattcat taatccacaa catagttcgt
     2101 tccggatgct tattactctt cggcatctcg aatacattgg tacccatctt tgtcgccatg
     2161 ggatttgcag gaaataacgg ttttgtc
//
LOCUS       DASVMB010000585         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_441652,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000585 DASVMB010000000
VERSION     DASVMB010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441652"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VF531_16325"
     CDS             complement(<1..1006)
                     /locus_tag="VF531_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX7715587.1"
                     /translation="MLKMLTGKKLLHALIAILAVITVAYTLPSGIILAKTSVKIYPGP
                     AEVPSATDREVYVNNAKLFVYDTEVNSNRAFSQNPTLGTTPVTYFDFGGGPVSIQVRA
                     PKVKINTVTIRPLALGIKPTIKNDTITFTLSKPAKLTIEINNSTERALHLFANPLEVN
                     APGPKTPGVIYFGPGVHKVGQINVDSNQTVYIAGGAVVYGWITPAMVNNVKIIGRGII
                     DGSIYDRWKDTRCPISVRSSSNVTVDGICIFNPSAWTLEAFKSTNVTFNNVKIIAARS
                     NSDGITVQSCTNFKCTDSFVRSWDDSLVVKGYDGDAMNYVFDNIQIWTDLAQSCEVGY
                     E"
     gene            1258..>2459
                     /locus_tag="VF531_16330"
     CDS             1258..>2459
                     /locus_tag="VF531_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7715588.1"
                     /translation="MTVTYRKLNMSYQKIVTSLKPGSCQVHEDYEIFFLLSGRGSIII
                     EQTAYHLKPGDLVIINNSEIHQDFLAEDKSGECIEIHFAPDLLTPFSQSFNLYHCFLD
                     RPKGERNKILLTKPQREKILKHFHKIGNLFANPFYADEVLKHTSLIELVFYINRLFLN
                     FSPIEERPNLPENLNAILEYIDDNIQNELSLEVLERTYYINKSYLGRLFQKYLNVSIH
                     EYIVLQRIAKAKELLLDGYSALEAGRLSGFNDYSNFSKMFKKVTRISPSEYVKKQHDE
                     NMNPRVLKPYRHKSHFKLPDLIAADLLWLPANPNIGDQIVFRAVVKNIGSGSVPIGVI
                     LGVGFMVDGFTETWSDNYTLGLAPGESIELNANNGQGGIATWTASRGAHQITAHVDDI
                     GRIDVLNR"
BASE COUNT          670 a          563 c          542 g          684 t
ORIGIN      
        1 tctcatagcc cacttcgcag gactgggcaa gatcggtcca tatttggata ttatcgaaaa
       61 cataattcat ggcgtcgcca tcataaccct tgacgaccaa gctatcatcc caggacctta
      121 caaaactgtc ggtgcattta aagtttgtgc atgactggac cgttattccg tcactattgg
      181 agcgggctgc aataattttg acgttattaa aagtaacatt cgtggattta aaggcttcca
      241 gagtccaggc cgaagggttg aagatgcaaa taccatccac cgtgacatta ctgctgcttc
      301 ggacgctaat cgggcacctg gtatccttcc agcgatcgta aatgctgccg tcaatgattc
      361 cccgtccgat gatcttcaca ttgttaacca ttgccggtgt gatccatccg taaaccacag
      421 ctccacccgc gatatataca gtctggttac tgtcaacatt gatctgccca actttatgaa
      481 caccaggccc gaagtagatg actcccgggg ttttgggacc gggcgcattg acttccagcg
      541 ggttggcaaa aagatgcaag gcgcgttcgg tgctattatt gatttcaatg gttaatttgg
      601 cgggtttgct caaagtaaag gtgatcgtat cattcttaat ggtcggctta atccctaatg
      661 ccagcggccg tatggtaacc gtgttgattt tgactttcgg cgcccgaacc tgaatggaaa
      721 cgggtccgcc cccaaaatca aaataggtga ccggagtagt tccaagtgtt ggattttgtg
      781 aaaaggcgcg attgctgttg acttcggtat cataaacaaa caacttggcg ttattgacat
      841 atacctcgcg atccgtagcg gatggaactt ccgccggacc gggataaatc tttacggaag
      901 ttttagccag aataatacct gaaggtagag tgtaggccac agtgatgaca gccagaatag
      961 ctatcaatgc atgcagaagt ttttttccgg tcaacatttt taacatgttt acctccccgt
     1021 gagtttgttt gaatttttac tggttttttc cctctcccac ttgaaacggt taacaatttg
     1081 ttaattcaag tatattacaa gacccgcttc taaaggttgt ttttcgagac taatgggatg
     1141 gtttatttcg actaaaaagg atctgtgccc gtattattga attaatagta cagatacatt
     1201 tgagatccgg tatcttgcgc gagagaggtt tctttcaaac cattccaagg agcggtcatg
     1261 actgtaacct atcgcaaact gaatatgagt tatcaaaaga tagttaccag ccttaaaccg
     1321 ggttcttgtc aggttcatga ggattatgaa atcttttttt tgctttcggg cagaggtagt
     1381 atcatcatcg aacaaaccgc ttatcattta aaacccggtg acctggtgat tattaataat
     1441 agtgaaatcc atcaagattt tttagcggag gataagtccg gcgagtgtat tgaaatccac
     1501 tttgctccgg acctattgac gcctttttcc cagtcgttca acttatacca ttgtttcctc
     1561 gaccggccca aaggggaacg gaataaaata ctcctcacca aaccccagag ggaaaagatc
     1621 ctcaaacact ttcataaaat cggaaacttg tttgccaatc ccttttacgc ggatgaggtt
     1681 ttaaaacata cgtcccttat tgaattggta ttttacatta accggttgtt cctaaatttc
     1741 tcgcccattg aggaacggcc aaatctcccc gaaaacctca acgctattct ggaatacatt
     1801 gatgacaaca ttcaaaatga actttccctt gaggttcttg agaggaccta ttatattaat
     1861 aaatcatacc tcggccgttt gtttcaaaaa tatctaaatg tgagtatcca tgaatatatc
     1921 gtcctgcaac gcatcgccaa agccaaggaa ctcctgctgg acgggtacag tgccctggag
     1981 gccggaagac tttcaggctt taacgactac tccaacttta gcaagatgtt taaaaaggtc
     2041 acccgtatct ccccctccga atacgtcaaa aaacaacatg atgagaatat gaatcccagg
     2101 gtgcttaagc cataccgcca caaatcccat ttcaagcttc ccgatctgat tgcggccgac
     2161 cttttatggc tcccggccaa tccaaatata ggagaccaga tagttttcag ggcagtggtc
     2221 aaaaacatcg gcagcggctc ggtacctatc ggggtgattt tgggggtcgg gtttatggtg
     2281 gatggtttca ccgaaacctg gtcggataat tatactttgg gattggcccc cggagaatcc
     2341 attgaattaa atgccaataa cggacagggc ggaatcgcaa cctggaccgc ctcgcgcgga
     2401 gcccatcaga ttacagcgca tgttgacgat atcggcagga ttgatgtgct taaccggga
//
LOCUS       DASVMB010000586        14917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_70901,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000586 DASVMB010000000
VERSION     DASVMB010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70901"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..622
                     /locus_tag="VF531_16335"
     CDS             143..622
                     /locus_tag="VF531_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEX7715589.1"
                     /translation="MTQPEFIGKINQAYQEAVHRGTRLNQAVVIAQAALESDWGNSVL
                     AQKANNLFSIKAGAYWTGKTLEMSTTEWNPTFGWYPAKAIWRSYPSWTDCILDYGAII
                     AGLPWYQDALSNLDDPRSFLKALLPNGAELGWATDPGYYDKVLRIAKQLEDFGGPKW"
     gene            complement(720..1319)
                     /locus_tag="VF531_16340"
     CDS             complement(720..1319)
                     /locus_tag="VF531_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HEX7715590.1"
                     /translation="MEKFSEFPVFPAKCVEEVPFSTSRRFETQFGQKLHFCEVPVSPK
                     QHFKLTTERVKVLKVIGEAVAQVVVEGNINLNAVKVDRVLAELRNVTDHVFPNKVVKQ
                     GTIHKQIFFVDPEGVVRHVTEDIPFMVAVDIPGLCPGPFIDVQNHLLDINTDFVLQPK
                     TCEKAAVLMQKVVAHILIKASKWTQMDVITNMEVFPKFF"
     gene            1780..3441
                     /locus_tag="VF531_16345"
     CDS             1780..3441
                     /locus_tag="VF531_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX7715591.1"
                     /translation="MPIKSENEPSFLGIQQLSLRGLLLGMLGSCIITASSMYVALRIS
                     ALPWPTVFVAVLSMALLKAFGKTTLNEINITQTGMSAGAMVAGGLAFTLPGLWITGLW
                     GGPEMLKTHFWPVLAIAMAGMLLGTVLTWFMRQRFVQKEALPYPIGAAAAETITVGDA
                     GGKKSGLLFGTMGLSAVFTYFRDGAGWIPATISSKWLYAKNFFVGIWLSPMAVGIGYM
                     IGPLFTGVWFLGAILSYLLIIPLGTAFHIFASTASAIVFKNTAGIGLMVGTGLGILLT
                     YIISRFQKPAITKNNGPEGTGSKVPSKQSGWVRSLTVLACAVTFILSVACGLGPLPSV
                     LLIIGTFAAAAMAATITGQTGINPMEVFAIIILLAIRLIVPVDTTGAFFIAAAVAVAC
                     GYSGDLLNDYKAGEMLGTNPVAQLISQIAGGIAGTVVAVLAMFAIINQFGGVGPAKGL
                     SAGQAFLVSQMIKGIGDPAVFGIAAAIGMLLYLLKVPVMTLGIGMYLPFEISAIVFSG
                     GLIRFIVDRVRPKAVEPGNIAASGLLGGEGITGVTIAIVKLLTRG"
     gene            3775..4638
                     /gene="coaW"
                     /locus_tag="VF531_16350"
     CDS             3775..4638
                     /gene="coaW"
                     /locus_tag="VF531_16350"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HEX7715592.1"
                     /translation="MKKIIGIDIGGSTTKIVGFDNGAIFSPLLIKATDPIASIYGAFG
                     KFISSNGLDLKDIERVMMTGVGSSFITSKLFGLPTGKVDEFRAIGMGGLFLSRLSRAI
                     IISMGTGTALVRADSHATPREAGVRHMGGTGVGGGTLLGLSNRMLNVRNFDDLIATAQ
                     GGSLTHIDLTIGDISHDELVGLPADTTASNFGKLSDLATKADIALGIINLVFQTIGMI
                     SVFATRIDHTRDVVLTGNLTNVPQAQEIFDGLSQLYDVNFVIPPHSEYATAAGAAICF
                     AEGMPFTDIGV"
     gene            complement(4759..6063)
                     /locus_tag="VF531_16355"
     CDS             complement(4759..6063)
                     /locus_tag="VF531_16355"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7715593.1"
                     /translation="MMIERIQTLLHENPEISGFKIIEELTDSFELFFIKKELDISRAK
                     QVQHYLITVYKDFAADGTSFKGSATVAVHPTMSDAELRVVLNEAAISAGLVKNKFYPL
                     VKPSGLKFSPEPSHFAGQPPAAWLSRLAQAVFQNDNYREGWLNSAEIFLNQVRLHLVN
                     SEGIDLTRESYRADLEAIATWREGASEIELFRELKFAEWNPDAIGGAIDQLLKHAKER
                     ALAQPTPGLKKHTVLLTGEPVREFFQYYYTQTSAQSVYEGVSTLRSGESIQGAEIRGD
                     RISFRLDPCLAGSTASAPFDEDGIPLEPVVIAEEGRLLRYWGDQRHSYYLGLNPTGRI
                     RNMAVEGGTQTLTTLKREPYLELVAFSDFQMDTLTGDFGGEIRLGWYFDGERTIPITG
                     GSISGNIREIEAEMYLSKELQQENEFVGPKTVKLPGVTVTGA"
     gene            complement(6063..7502)
                     /locus_tag="VF531_16360"
     CDS             complement(6063..7502)
                     /locus_tag="VF531_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7715594.1"
                     /translation="MRVEFSPFLTDQKENLRQLIAALTPYFSYVSVLGTDNSGKQFLV
                     QKTGSNVSDSRWTERGFVVRVYDGARGRYFEYSYNQPLPAATLAETIRAKANEAFTSV
                     DSSGIHSIPYPVINEKPLQHSYHGEVAVPPGSISTAAKVRQLTAIHEKALSYPMKSGD
                     LVDCRVRYEEVYVSKIFVSKQRDLEQSYIWSQGYLIPIVRRDGQTKFCYRSFSGLHGP
                     ELLETMTAAPHAAVALAERLLDAKPAEPGEYNVICSPEVAGLIAHEAFGHGVEMDMFV
                     KNRAKAVEYLGKPVASNLVTMHDGAAAASHVSSYFFDDEGNSATDTVVIDRGILKAGI
                     SDQLSAMKLGTVPTGNGKRESFERKAYARMTNTFFAAGTDRLNEMIGSIRHGYLLDGM
                     MSGMEDPKNWGIQCMILWGEEIRDGKLTGNLISPVVMTGYVPDLLKAVSMVSTDVELF
                     GSGACGKGHKEWAKVSDGGPYIKTRARLG"
     gene            complement(7521..9041)
                     /gene="pepD"
                     /locus_tag="VF531_16365"
     CDS             complement(7521..9041)
                     /gene="pepD"
                     /locus_tag="VF531_16365"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HEX7715595.1"
                     /translation="MARFFEGVEPERVWYHFEELTKLPHGSGNEDAVRKYLADFAQKH
                     GFDHFYNPKASVDQPGERVMVIYKKAAPGLENKPTVVIQGHMDMVCVPDEQIFPLKLV
                     HCDAQGQPGKGWVKAGGYTDQDGTSLGADNGIGMSIALAILEDTGHRFGPIECFFTVQ
                     EEVGMDGARDFNPDLLKGRVYLNLDEETLQTVTYGCAGGLRIDLSWEMKRESVPAGAK
                     CYSLNVSGLKGGHSGARIHEGRANAIKLLTRILYQARDSKIEYTIASLNAGNIKQSNV
                     IPGNARAHVILKPQDVPDFEKLVATMRETFTAEYRGIEPLLEVDWQEIDAPETMMSPS
                     DDRRLADVLMMAPHGVVKLTPGNPELVETSTNLAAVSVNGVEAKVVAMPRSSRQSGVE
                     WVWNIYQAIAASTGVNLSRSEWYPSWTPREDSTLLQTAKEIYSERFHGDFKAIVIHAG
                     LECGWIVNKYEKGTPMDCIAFGPNLVDPHSRRERVEIASVAAFYQCVLAILKHYAE"
     gene            complement(9423..9830)
                     /locus_tag="VF531_16370"
     CDS             complement(9423..9830)
                     /locus_tag="VF531_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX7715596.1"
                     /translation="MDRYIFPAIFEPGENKGYTVNFPDLPGCITEGDTMEEALHMAKE
                     ALELHLYGMEEDKDEIPNPSRPEELKVPEKSFVSLVEVWMPIIRDEMENKAVKKTLTI
                     PKWLNDVAEKNNVNFSQVLQSALKEYLGILKHE"
     gene            complement(9846..10040)
                     /locus_tag="VF531_16375"
     CDS             complement(9846..10040)
                     /locus_tag="VF531_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX7715597.1"
                     /translation="MKIYSSQEILQILYKDGWTIKNQRGSHIQLIHPTKLGKVTVPHP
                     KKELDLKTVKSIFKQANIDN"
     gene            complement(10239..10754)
                     /locus_tag="VF531_16380"
     CDS             complement(10239..10754)
                     /locus_tag="VF531_16380"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7715598.1"
                     /translation="MKDLKKSIPSIITALRLLSAPFFYYLFLNSTCLLAFLIFVGAAI
                     TDIIDGRLARKYNATSVFGSYLDVCSDFILIICAFLAFIKIKWYCYLLILIIIASFAI
                     FILTSRKKKLIYDPVGKYTGTILMVSILLTMLIPYPLIRKIITYIIALSFVTSICFRI
                     HFLYKTKGICY"
     gene            complement(10899..11852)
                     /locus_tag="VF531_16385"
     CDS             complement(10899..11852)
                     /locus_tag="VF531_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7715599.1"
                     /translation="MATAKSRGHPSRQLKGEDHIDGIKILNFGKKNPPDADLVSSTVD
                     LNNEGLPTLTPYLLKEEKLHPAIIVCPGGAFQYRASHEGEPIAKWLNEIGINAFVLNY
                     RVAPYTPFTSTKDAVRAVRYIRYHAPELNIDPDRIAMMGFSAGGYLTAFIGTHFDNGM
                     IEPESRNAQIMSILLGELDFSDPIAQTSSKLNALILCYAETSPFSFSKEKLEKLPPGS
                     LLPKDITLDEFIDFTSTHKHVTAKTPPAFLWVTATDDFNFQRQNLNFAQALSEQDLSF
                     EFHIFSKGHHGLGLGKDEPAAAIWPKLCENWLRGLWSASFS"
     gene            complement(12115..12726)
                     /locus_tag="VF531_16390"
     CDS             complement(12115..12726)
                     /locus_tag="VF531_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7715600.1"
                     /translation="MELKIESQNLDEYLKSSDVIDYNSLNIVSLAKSLSEGMENEIEI
                     TERVYEYVRDEIAHSGDIKTSIVTCNASDVLEQKHGICCAKAHLFAALLRYLNIPVGF
                     CYQWLISDNEDHSLVIHGLNAVYLRDIKRWIRLDARGNKEGVNAEFSIDTEKLAWPVR
                     YGLGEKEDPVVYFEPKKEVIEVLRSSKDRKELSHKWITANSGR"
     gene            complement(12821..13657)
                     /locus_tag="VF531_16395"
     CDS             complement(12821..13657)
                     /locus_tag="VF531_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX7715601.1"
                     /translation="MAIDKQYLSDFEAILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGMEPTGLLLKECADLIFSAWKEDGSFKLYPQGSIYPCHTAHAADVLCHMGY
                     AYDTRLQKTFQHLLDIQYTDGGWRCNKFSFGRGPETQYSNPFPTLTALNAFRFSDYLN
                     KEPALDRAVDFLLEHWTIRKPIGPCHYGIGTLFMQVEYPFRNYNLFVYVYVLSFYDRA
                     KEDNRFLEALKALESKMVDGQIVVERIVPKLAKFSFCKKGEPSALATIRYHEILKNLG
                     IK"
     gene            complement(13850..13981)
                     /locus_tag="VF531_16400"
                     /pseudo
     CDS             complement(13850..13981)
                     /locus_tag="VF531_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(14091..14744)
                     /locus_tag="VF531_16405"
     CDS             complement(14091..14744)
                     /locus_tag="VF531_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7715602.1"
                     /translation="MNIIPDQEQYWDKVSEEKEFPTPFQIGEFGKYVSKEMNILDIGC
                     GYGRTLNELYNSGFKNLVGIDFSRGMINRGLKMYPHLKLQKNDNGILPFPDNSFDAII
                     LIAVLTCIADGNEQHKLIAEILRVLKTAGLLYINDYLINQDQRNIARYNKYKEKYGTY
                     GVFELSERGVMAVVRHHTKEHIFEITKDFQEITFKPVLYTTMNGNKSNGFYYIGKKK"
BASE COUNT         3829 a         3678 c         3217 g         4193 t
ORIGIN      
        1 ccttgatatt ttttgattga ttgaacagtc aatcatcggt ttgagtatag atcgagttgg
       61 aaaaaatgtc aatataaatc aagcagcaat tgatcccggt tcacatagat ctatatatat
      121 gcctaaaaat gaggttgata aaatgaccca acccgaattt atcggaaaga taaatcaggc
      181 ttatcaggaa gctgtccatc gtggaacccg cctgaatcag gcggtggtta ttgcccaagc
      241 cgcgctggag tcggactggg gcaattcggt gcttgcccaa aaagcgaata atcttttctc
      301 gattaaagcc ggagcctatt ggaccggtaa aaccctggaa atgtctacaa ccgaatggaa
      361 ccccacattc ggctggtatc ctgccaaggc aatctggcgg tcttatccga gctggaccga
      421 ttgtatcctg gactacgggg caatcatcgc cggattgcca tggtaccaag atgcgttgtc
      481 taacctggat gatccgcgtt catttttgaa ggcgcttctg ccgaacggag ccgagttagg
      541 ttgggccacc gatcccggat attatgataa ggtactgcgg atcgcaaaac aacttgagga
      601 tttcggcggt cccaagtggt aagcctggat agagtcacaa aaaaaagatc agcttggtgg
      661 ctgatctttt tttatttaac acaagagtga attgcgctta gccattatta tcttttgaat
      721 caaaagaact tcggaaagac ctccatattg gttattacat ccatttgggt ccatttcgac
      781 gctttaatca ggatgtgggc gactactttc tgcatcagga cggccgcttt ttcgcatgtc
      841 ttcggctgga gtacaaaatc ggtattgatg tctaacaggt gattctgaac atcaatgaac
      901 ggtccagggc agagtccggg aatatccacc gctaccatga acgggatatc ctcggtgacg
      961 tgacgtacta ctccctcagg atctacaaag aagatttgtt tgtgaatcgt accttgtttg
     1021 acaaccttat tcgggaagac atgatccgtc acgttgcgca attcggccag aacccgatcg
     1081 accttcaccg cattaagatt gatattcccc tcaacgacta cctgagcaac ggcctctcct
     1141 atgactttca gcactttcac ccgttcggtg gtcaatttaa aatgctgttt cggactgacc
     1201 ggaacttcac aaaaatggag cttttggccg aactgagttt caaaacgcct tgatgtacta
     1261 aacggaactt cttctacaca tttcgccgga aaaaccggga attcactgaa tttctccaaa
     1321 acattgaccc tcctttgcaa gggcttcccc tctgacttga gttatccccc ttttaattcc
     1381 tatagtatgg cacttctgtc cgagtcgaac cgggacatac atactatttc aaaaagttat
     1441 tacctttttc cctattgcaa tcgcccatat caaccgggcc atcatctcaa agtgaaccat
     1501 tttgaagggg ccattgaaaa actgcgcatt tccccaattt tatggtgagt gggaaggaga
     1561 gttgcggaat accgtaaaat atggtatgtt attataaatt ttatccatat gaggtaccct
     1621 gaggtaagga cggaaatgcc atacgtcgtc gaagcttttc gaatctattt gggtccatct
     1681 atccggccat ggcattatgt aattgatctc atccggttta ttattgagta tagatgattt
     1741 gttgatttct gagtttattg gaggggggtt tttttataaa tgcccatcaa atcagagaac
     1801 gagccgagtt ttttaggcat ccagcaactg tcccttcgtg gcctactcct ggggatgttg
     1861 ggttcttgca tcatcaccgc cagttccatg tatgtggcgc tccggatcag cgcgcttccc
     1921 tggcccactg ttttcgtggc cgtactctca atggccctgc tcaaagcatt cggcaaaacc
     1981 accttaaatg agatcaatat cacccagacc ggaatgtcgg ccggggcgat ggtcgcgggc
     2041 ggtcttgctt tcacactgcc cggtttatgg attaccggtc tctggggtgg cccggagatg
     2101 ctcaaaaccc atttttggcc ggtgctcgcg attgcaatgg ccggtatgtt attgggcacc
     2161 gtactgacct ggtttatgag gcaaagattt gtgcaaaaag aagcgctgcc ttatccgatt
     2221 ggggcagcgg ccgcggaaac catcaccgta ggagatgccg ggggtaaaaa atccggtctg
     2281 ctctttggga ccatgggtct gtccgccgtt ttcacctatt ttcgcgatgg agcggggtgg
     2341 atcccggcta caatctcatc caagtggctt tatgccaaga acttttttgt cggaatctgg
     2401 ctttctccca tggcggtagg gatcggttat atgatcgggc cgctcttcac aggggtttgg
     2461 tttctcgggg ctatactctc atatctactc attattccgc tgggaaccgc tttccatatc
     2521 ttcgcctcga ccgccagcgc gattgtattt aagaatacag ccgggatcgg cctgatggta
     2581 ggaaccggtc ttggtatttt acttacatat atcatttccc gctttcaaaa acctgcgatt
     2641 acgaaaaaca atggtccgga aggaaccggt tcaaaagtcc cctctaaaca aagcggatgg
     2701 gtacgcagtc tgacggttct tgcctgtgca gtgaccttta tactctcggt agcctgcggg
     2761 ctcggccccc tgccatccgt gctgttaatc atcggaacct tcgcagccgc cgcaatggct
     2821 gccaccatta ccggccaaac cggcattaat ccgatggaag tcttcgcaat cattatttta
     2881 ctggctatcc gcttaatcgt tccggtcgat accaccggcg ccttctttat tgcggccgcc
     2941 gtcgccgtgg cctgcggtta ttcgggcgat ttactgaacg attataaagc cggcgagatg
     3001 ctggggacga atccggtagc ccaattaatc tcccaaattg cagggggtat tgccggaact
     3061 gtggtagcag tgctggcaat gttcgccatc atcaatcagt tcggcggcgt gggaccggcc
     3121 aagggattat cggccgggca ggcttttctt gtaagccaaa tgatcaaggg tatcggtgat
     3181 ccggcggtct ttggcattgc cgcggcaatc ggcatgctat tgtacctctt gaaggtgcct
     3241 gtcatgacct tgggcattgg aatgtatttg cccttcgaga tctcggcgat tgtattcagc
     3301 ggcgggctga ttcggttcat cgtcgaccgg gtgcgcccca aagcggtgga acccggaaat
     3361 atcgctgcct ccggtctttt gggcggtgag ggcatcaccg gggtgacgat tgccattgtg
     3421 aagctgctga cgaggggata aggcatgagg catgagatac gaggcaacag gtaacaggca
     3481 atagggaaaa ggtagaaatg acataggtcg gcttcgtcgt ctacgactca ctctcgctct
     3541 ctctaacgtt tgtcgctaga gtcgcgcaca cgtgcgcgcg taacccgtat cctccggtgt
     3601 ctatttcact tctttgaacc agacttctcc cctctttacc gctttgtggt agagcggaga
     3661 aagtagggtg agtcgcctca cgcctcgtac ctattccctg ttgcctgttc cctgttccct
     3721 gttccctggt ccctgttccc tattgcctat attataccaa ggagcatttt caggatgaaa
     3781 aagatcatcg ggattgatat cggcggtagc accaccaaga ttgtgggttt cgacaacgga
     3841 gcgatcttta gccccctact catcaaggcc accgacccaa tcgcctccat atacggggct
     3901 ttcggcaagt tcataagcag caatggcttg gacctaaagg atattgaacg ggtcatgatg
     3961 accggggtcg gatcatcctt catcacttcc aaattatttg ggcttcctac cgggaaagtc
     4021 gatgaattcc gggccattgg catggggggg ctctttttga gcagactctc ccgggctatc
     4081 atcatcagca tgggtacagg aacggccctt gtaagagccg acagccatgc cactccccgc
     4141 gaagccgggg tccggcatat gggcgggacc ggggtcggcg gcggcacctt gttgggactc
     4201 tccaaccgga tgctcaatgt ccgcaatttt gacgatctga tcgctaccgc ccaaggcggc
     4261 agtctgaccc atatcgacct aaccatcggc gatatctccc atgatgagtt ggtaggatta
     4321 ccggcagata ctactgcctc caacttcggc aagttgagtg atctggcaac caaggccgat
     4381 atcgcgctgg gcatcattaa tctggtattc cagaccattg gaatgatttc ggtattcgcc
     4441 acccggatcg accatacccg ggatgtggtg ttgaccggga atctgaccaa tgttccgcag
     4501 gcgcaggaga tttttgatgg attaagccag ttgtatgacg tgaatttcgt gatacccccc
     4561 cattcggagt atgccacagc cgccggcgca gccatctgct tcgcggaggg aatgcctttc
     4621 accgatatcg gggtgtgatt tttcggtttg aattaacttt tgaactaatt aagtaaagcc
     4681 gccaaagaaa caccgattgg cggcttttat cgtgaaaata ttcctaacaa ctttgattcc
     4741 aaaacgaaat aataccgact aagccccggt taccgttacc ccgggcaatt taaccgtttt
     4801 cggcccgacg aactcattct cctgctgcaa ctcctttgag agatacatct ccgcttcgat
     4861 ttcacggata ttaccggaga tggaaccgcc ggtgatcggg atagtacgtt caccgtcgaa
     4921 ataccacccc aaacggatct ccccaccgaa atccccggtc agagtatcca tttgaaaatc
     4981 ggaaaaagcc accaattcca aatacggctc tcgctttagc gtggtgaggg tttgggttcc
     5041 accttcgacc gccatgttac gaatccggcc tgtggggttc aaccccagat aataagagtg
     5101 gcgctggtcc ccccagtaac ggagtaaacg gccttcttcc gcaatcacta ccggttcgag
     5161 gggtatacca tcctcatcga acggggcgga agcggttgaa ccagccagac aggggtccaa
     5221 ccggaaactg atccggtcac cccggatctc cgctccctgt atggactcgc cgctccgtag
     5281 agtggataca ccctcatata cgctctgggc ggaggtttga gtatagtagt actggaaaaa
     5341 ctcccgtacc ggttctccgg tcaacaaaac cgtatgtttt tttaatccgg gtgtcggttg
     5401 ggccaatgcc ctttctttcg catgctttaa caattgatcg atggctccgc cgatggcatc
     5461 cgggttccac tccgcaaatt ttaattcccg gaataactca atctccgaag ccccctcccg
     5521 ccaagtggcg atggcctcaa gatctgcccg atagctttcg cgggttagat caatcccttc
     5581 ggaatttacc aagtggagcc gaacctgatt taaaaagatt tcagctgagt ttaaccagcc
     5641 ctcccggtaa ttatcgtttt gaaaaacggc ctgtgccaag cgggacaacc aggcagccgg
     5701 cggttgcccg gcaaaatgac ttggctcggg ggaaaatttg agaccggacg gtttgaccaa
     5761 gggataaaac ttattcttca ccaatcccgc actgatggcc gcttcattaa gcaccaccct
     5821 gagctccgca tcggacatgg tcggatggac tgctactgta gccgaacctt taaaactcgt
     5881 accatccgcc gcaaaatctt tataaaccgt tatcaggtaa tgctgtacct gtttggcccg
     5941 gctgatatcc aactcttttt taatgaagaa taattcgaag gaatcggtga gttcctcgat
     6001 gatcttaaac ccgctgatct ccggattttc atgtaaaagg gtttgaattc tttcgatcat
     6061 catcagccca acctcgctct cgtcttgata tagggtccgc cgtccgatac cttggcccac
     6121 tccttatggc ctttaccgca agcaccggat ccgaataact caacatcggt tgagaccata
     6181 gaaaccgctt ttaaaagatc ggggacgtat ccggtcataa ccaccggaga aataagattt
     6241 cccgtcagtt tcccgtcacg aatctcttca ccccagagga tcatgcattg gattccccag
     6301 ttcttggggt cctccatgcc gctcatcatc ccgtccagca aatacccgtg gcggatcgag
     6361 ccgatcatct cgtttaaccg atccgttcct gcggcaaaaa aggtattggt cattcgggca
     6421 tacgcttttc gttcgaatga ctcccgtttg ccgttaccgg taggaacagt tccaagtttc
     6481 attgccgaca gctgatcgga gatgcccgct ttcaatatgc cgcggtcgat tactacagta
     6541 tccgttgcgg aatttccctc atcatcaaag aaatacgagg atacatgact ggccgcggct
     6601 gcaccgtcgt gcattgtgac aaggttggat gccaccggct tgccaaggta ctccacggct
     6661 ttggcccggt ttttcacgaa catatccatc tccactccgt gaccaaacgc ctcatgggca
     6721 atgaggccgg cgacttccgg agaacagatc acattatact cccccggttc cgccggcttg
     6781 gcatcgagta accgttccgc caaagccacg gcagcatgcg gagcggccgt catagtttcg
     6841 agcagttcag gcccgtgtaa cccggaaaaa ctccggtagc aaaacttcgt ctgcccatcg
     6901 cggcgcacaa taggaatcag gtatccctga ctccaaatat aggattgctc caagtccctc
     6961 tgtttggaga caaagatctt ggatacgtaa acttcttcat accgtaccct gcaatctact
     7021 aaatcgccgg atttcatagg gtaggaaagg gctttttcat gaatggcggt caactgcctt
     7081 acttttgcgg cagtgcttat tgaaccggga gggactgcga cttccccgtg atagctatgc
     7141 tgtaatggtt tctcgtttat aaccggatac ggtatggaat gaatcccgct tgagtcaaca
     7201 ctggtaaacg cttcattcgc cttggcgcgg attgtttccg ccaaagtcgc ggcgggtaaa
     7261 ggctggttgt aggagtattc gaaatacctc ccgcgcgcgc catcgtaaac ccggaccaca
     7321 aacccccgtt ccgtccaacg ggaatccgaa acattgctcc cggtcttctg gaccaggaac
     7381 tgtttgcccg aattgtcggt gcccaatacg gaaacatatg aaaaataagg agtaagcgcg
     7441 gcaatcaatt gccgcagatt ctctttctga tccgttaaaa acggggaaaa ctccactcgc
     7501 atgttcacta ccacctttcc ttactcggcg taatgtttca agatcgccag cacgcactgg
     7561 taaaaggcag ccacactcgc gatctccacc cgctccctgc ggctatgggg atccaccaga
     7621 ttcggaccaa aggcgataca gtccatcggt gtcccttttt catatttatt cacgatccag
     7681 ccgcactcaa ggcctgcgtg gatcacgatc gccttaaaat cgccgtgaaa ccgttcgcta
     7741 tagatctcct tcgccgtttg taaaagggtt gagtcctccc ggggagtcca ggaaggatac
     7801 cactcgctcc gggaaaggtt cacccccgtg gatgcggcga tagcctggta tatgttccaa
     7861 acccattcaa ccccgctctg ccgggaactg cggggcattg ccaccacttt cgcctccaca
     7921 ccgtttacgg atactgccgc caggttggtg gatgtctcga ccaactccgg gttgcccggt
     7981 gtaagcttca caaccccgtg cggagccatc atcaatacat ccgccaggcg acggtcgtcg
     8041 cttggactca tcatcgtttc tggtgcatct atctcctgcc agtcaacctc aagcaagggt
     8101 tcaatgcccc gatactccgc ggtaaaggtc tctctcatgg tggcgacgag cttctcaaaa
     8161 tccggcacgt cctgaggttt taggatgacg tgagcccggg cattcccggg aataacattg
     8221 ctctgtttga tattgcccgc gttcaaagaa gcgatggtgt actcgatctt gctgtcacga
     8281 gcctggtata agatccgtgt caaaagtttg atcgcattgg ccctgccttc gtgaatcctc
     8341 gctcccgaat gcccgccctt gagaccggag acattgagcg aataacactt ggctccggcc
     8401 ggaaccgatt cgcgcttcat ctcccagctt aagtcgattc taagcccgcc cgcgcaaccg
     8461 taagtaacgg tctgaagtgt ttcttcatcc aggttgaggt aaacccgccc cttcagcaga
     8521 tcggggttga aatccctggc accgtccata cccacttctt cctggaccgt gaagaaacac
     8581 tcgatcggcc cgaaacggtg cccggtatcc tcaaggatag ccagggcaat tgacattcca
     8641 atcccattgt ccgctccgag gctggtgccg tcctgatcgg tatagccgcc cgctttgacc
     8701 caacccttgc ctggttgtcc ttgagcgtca cagtgaacca gcttaagcgg gaagatctgc
     8761 tcatcaggga cgcagaccat atccatatgt ccctggatca cgacagtcgg cttgttctca
     8821 aggccagggg cggctttctt ataaataacc attacccgtt caccgggttg atcaaccgag
     8881 gctttcgggt tgtaaaaatg atcaaagcca tgcttttggg caaaatcggc caaatacttc
     8941 cgaaccgcat cctcattccc ggaaccgtgc ggcagtttgg tcaattcctc aaaatgatac
     9001 caaacccgtt ccggttccac cccttcaaaa aaacgcgcca tttggcaacc ccctctttat
     9061 gtatagaaca agattagtat ggttaagaac aacgatttac ttactgcagt tatcattcta
     9121 ccacaaaaat ggcaaaagcc ctatgccaca aaaaaatgat ccctgccatc gtttgcggtg
     9181 aattccaccg gcaaacaaca gtgatcatga aacagcttca ttaaaataat attgtattta
     9241 aatcttattc gaatcacagt ttcagtaaag gggaaacata taagccttca ttatcacttg
     9301 gtacatgtgg cgcgacctat cgataaaccg cgccccgcta gcttgagtgc aatgttgaat
     9361 acaggactgt gcccagccgg agccaaggat ggtggaggcg tctcttacga aaccaactaa
     9421 ttctattcat gttttaaaat acccaaatac tctttaagag cgctttgcaa tacttgagaa
     9481 aaattaacat tgttcttttc cgctacatca tttaaccatt ttggaatggt caaagttttt
     9541 ttaactgcct tattctccat ctcatccctt ataattggca tccaaacttc taccagactt
     9601 acaaaggatt tttcaggaac ttttaattct tcaggccgag agggattggg aatttcatcc
     9661 ttatcttctt ccattccata taaatgaagt tccagagctt cttttgccat atgcaaagct
     9721 tcttccatag tatcgccttc agttatgcat ccaggcaaat ctggaaaatt cacagtatat
     9781 cctttgtttt caccgggttc aaaaatcgcc ggaaaaatat acctatccat tataaatacc
     9841 tcctcttagt tgtctatgtt tgcttgttta aaaatgcttt taactgtctt tagatctaat
     9901 tcttttttgg ggtgagggac tgttactttt ccgagttttg ttggatggat aagttgaata
     9961 tgagaacctc tttgattttt aatcgtccaa ccatctttat atagtatttg taatatttct
    10021 tgtgaggagt aaattttcaa ctttttccct cccctataat ataactataa cacgtgtctt
    10081 tcatacgtgt caatatagac tgaagctttc tcgcattcaa aggtccccct atacaaaagg
    10141 caccctgacc ttttcaaaac ctggtggcaa caccgactcg gtttggagaa tttaggcaag
    10201 acagcttcat gaataaactt cagggatagc gagagcggtc aatagcatat tcccttagtt
    10261 ttatataaaa aatgtattct aaaacatatt gaggtaacaa atgataatgc aatgatatat
    10321 gtaattattt tccgtattaa tggatatggt attaacattg ttaataaaat acttaccatc
    10381 aatattgttc cggtatattt ccccaccgga tcatatatca actttttctt gcgcgatgtt
    10441 aatatgaata tggcaaatga agctataatt atcagtatta gcagataaca gtaccattta
    10501 attttgataa aagctaaaaa ggcacatatt attaatataa aatcggaaca tacatccaaa
    10561 tatgatccaa atactgaagt tgcattatat tttcttgcca atcttccatc aattatatct
    10621 gttattgctg caccaacaaa aattaggaat gccagtaaac aagtagaatt tagaaaaaga
    10681 taatagaaaa atggtgcaga aagaagtctt agtgcggtaa taattgatgg tatgcttttc
    10741 ttaaggtctt tcataagaaa cccccactca tgttctatgc agtccgccag gatagcagtc
    10801 tgctttaatg accgcgccat atacaatgtc gcctaacatc cgtccgttca gccagccctc
    10861 agtcacggtt gtccaaggag ggacacagcc tcgaaatatt atgaaaaact tgctgaccat
    10921 agtcctcgaa gccaattttc acaaagcttt ggccagattg ctgccgcggg ttcatcctta
    10981 cctaatccca aaccatggtg acccttggaa aatatatgga attcaaatga tagatcttgc
    11041 tcacttagag cttgagcaaa gttcaagttt tggcgctgaa aattaaagtc atcggtcgcc
    11101 gtaacccata gaaaagccgg tggtgttttc gcagtaacat gtttatgggt tgaagtaaag
    11161 tcgataaatt catccaatgt aatatctttt ggcaataaag aacctggcgg caatttttcc
    11221 aatttttctt tagaaaacga aaatggggat gtctcagcat aacataaaat gagcgcattg
    11281 agtttagaac ttgtttgagc aatcggatcg ctaaaatcca attctcccaa aaggattgac
    11341 ataatttgcg cgtttcggct ttccggttct atcattccat tatcaaaatg agttcctata
    11401 aaagcagtga gataaccgcc ggctgaaaat cccatcatgg cgattcggtc cggatcaata
    11461 tttaattccg gggcatgata acgaatatac ctaactgcgc gtactgcatc tttagttgaa
    11521 gtaaatgggg tataaggagc gacccggtaa tttaaaacaa aagcattgat tcctatttca
    11581 ttcagccatt tcgcaatggg ttctccttca tgagacgctc tatattgaaa tgctccaccc
    11641 gggcagacaa taattgccgg atgaagtttc tcttctttta gtaaataagg ggtcagggta
    11701 ggtaaccctt cgttattcaa gtccacggta gaggaaacta agtcggcatc aggaggattt
    11761 tttttcccaa agtttaatat ctttatccca tcgatatgat cctccccttt taattggcgg
    11821 ctcggatggc cacggctttt cgcggtagcc acttcttaat caaccctatg aatgcctggt
    11881 cgtcaacccg ctgttccacc atcttgataa gccgctcgtg gtcaatgttt tcaaaccttc
    11941 attcaatgcg cgcttaccac tatctttact ggagaaatag ttcctaccca aattagaatc
    12001 aataagggct ttttcacatt cattcaaata tcgcttcttt gctttggtta tgattaggtt
    12061 cataaaaaat ctccttttat tgtatgcctg tgattttgta gcggcacgat gccgctatcg
    12121 gcctgaatta gcggtaatcc atttatgact caattctttt ctatctttac ttgatcttaa
    12181 aacctctatg acttcctttt taggttcgaa atatactacc ggatcctcct tttcccctaa
    12241 tccatatctc acaggccaag ctaacttttc agtgtcaata ctaaactcag catttactcc
    12301 ttctttattg cctcttgcgt ccaacctaat ccatcttttg atgtccctta gatataccgc
    12361 gtttaaacca tgaataacta atgaatgatc ttcattatca gatattaacc attgataaca
    12421 aaacccaact ggaatattta aatagcgtaa gagagccgca aatagatgag cttttgcgca
    12481 acaaattcca tgtttttgtt ccaatacatc agaagcatta caagttacaa tacttgtttt
    12541 aatgtccccg gaatgtgcta tttcatccct gacatattca taaactctct cggttatttc
    12601 aatttcattt tccattcctt cagatagtga tttcgccaaa ctcacaatgt tcaaagagtt
    12661 gtaatcaata acatcactac tttttaagta ttcatctaga ttttgacttt caatctttaa
    12721 ttccatttgt ttttcccctt aaaaagtttt aacggagcgg catgaaaaca gctatcgacc
    12781 acatttagca cttgcttgat taatcatcaa atgttggtta tcactttatg ccaaggtttt
    12841 ttaagatttc gtggtagcgt attgttgcca atgcacttgg ttcacctttt ttacagaaag
    12901 aaaacttggc cagcttcgga acaatgcgtt caacaacaat ctgaccatcc accattttgg
    12961 attccagcgc ttttaaggct tccagaaacc ggttgtcctc ttttgcgcgg tcataaaagg
    13021 ataacacata gacgtaaaca aaaagattgt agttgcgaaa gggatattct acctgcatga
    13081 acaatgtgcc tattccgtag tgacatgggc caatcggttt ccggattgtc caatgttcaa
    13141 gcaagaaatc cacagccctg tcaagcgcag gttctttgtt gagatagtca ctaaagcgga
    13201 atgcgttcag ggcagtaagt gttgggaatg ggtttgaata ctgagtttct ggaccccgac
    13261 caaagctaaa cttattgcaa cgccaaccgc catcggtata ttggatatct aaaaggtgtt
    13321 gaaaagtttt ttgcaatctg gtatcgtaag cataccccat gtggcaaagt acatcggcag
    13381 cgtgagcggt gtggcatggg taaatagagc cttgcggata cagcttaaag cttccatctt
    13441 ctttccatgc actaaatatc aagtcggcgc attctttaag taaaaggccg gtaggttcca
    13501 tacccaattc taacagataa agtacgctgt ccagagtgga gaaaggcgat ccttttatta
    13561 gacgtttgtc gggagtagtc caatagtcag cgccattatc atacctgtgt gacaatatcg
    13621 cttcaaaatc tgataaatat tgcttgtcta ttgccattta ttatcactcc attttcgatt
    13681 ctgctttccc ggacgggtct tacaggactt ggcgcggacg gtttgggtcg atgccggaaa
    13741 ttcccagatt ccttacaaac cggcacggag cctgcaacac gttgttacac gccgtaaccg
    13801 ccaccaatca atagccatgg agggcactat tactttattg ctgaatatct taattacatt
    13861 tacaatatat ttcgatatca tttccttcac agtctttaaa ttctaacacc caattatcat
    13921 tgattcttgt cagcggaaat attattcttg cgtttagatt gctgagtttt tctagtagta
    13981 gtttatggcg gtacgaggcc gccatttttc ttcgaatacc gcgccccgac gcagtatatg
    14041 acgtaccgtc ctcagttcaa ggttgtccaa ggagggacaa ccacttacat ctatttcttt
    14101 ttcccgatat aataaaatcc attcgattta tttccgttca ttgttgtata aagcacaggc
    14161 ttaaaagtta tttcttgaaa gtcttttgtt atttcgaaaa tatgttcttt tgtatggtgt
    14221 cttacaacag ccataacgcc tctttcagaa agttcaaata caccatatgt accatacttt
    14281 tctttatact tgttatatcg tgcaatattc cgctggtcct gatttatcaa gtaatcgttt
    14341 atatataaaa gccctgctgt ttttaaaact cttaagattt cagctattaa cttatgctgt
    14401 tcattcccgt ctgcaataca tgttaatacg gctatcagta ttattgcatc aaaagagtta
    14461 tccggaaaag gtaaaatacc attatcattt ttttgtaatt ttagatgagg atacattttt
    14521 agtcctctat ttatcatacc ccgtgaaaaa tcaatgccaa caagattttt gaaacctgaa
    14581 ttataaagtt catttaaagt tcgcccataa ccacatccga tatctaaaat attcatttct
    14641 tttgaaacat acttgccaaa ctcaccaatt tgaaaaggtg ttggaaattc tttttcttct
    14701 gatactttgt cccaatattg ttcttgatca ggtattatat tcatctttgc ttcccctttt
    14761 taaatcagcg gcctggccat cctgattcaa atcgtatttg tccaaatccc attgatagta
    14821 gatgtcatca aacattgcta agccaaccac caaaacataa accggcgccg tttccaaccc
    14881 aactcaactg ccaatgatgg caaaatgttt taaataa
//
LOCUS       DASVMB010000587         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_733690,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000587 DASVMB010000000
VERSION     DASVMB010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733690"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..240
                     /gene="tnpA"
                     /locus_tag="VF531_16410"
                     /pseudo
     CDS             1..240
                     /gene="tnpA"
                     /locus_tag="VF531_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(391..477)
                     /locus_tag="VF531_16415"
     tRNA            complement(391..477)
                     /locus_tag="VF531_16415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Leu,seq:caa)
     gene            complement(602..1210)
                     /locus_tag="VF531_16420"
     CDS             complement(602..1210)
                     /locus_tag="VF531_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX7715603.1"
                     /translation="MNEKSYLTVQTPAKAELTVEHSRFIGHCREVASETEAKAFIAQI
                     RAENSQANHNCYAYRLGAGKDPLEYYNDHGEPSGTAGKPILGAIERLKLTNVVVVVTR
                     YFGGKKLGVRGLIDAYGQTATLVLKTAGSCVRIPTFCTTLTCSYADHSLILHRLKQVD
                     PTVADTGFTASVTLHLVIPEANRTVFESILKELPVQDVSYTD"
     gene            complement(1247..1681)
                     /locus_tag="VF531_16425"
     CDS             complement(1247..1681)
                     /locus_tag="VF531_16425"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HEX7715604.1"
                     /translation="MRRNYYLLGISLVALALIAGFGMATKKPEIRIKAALDQTVYRKN
                     TPIGVAVLIYTTKDTQIEFSSAKLYDLILEKDGKTIWQLSDMKMYAQSLMKRDFKAGE
                     PQIYSTVIDASQIKLEPGHYLLKPVFCGNPAITGQPVGLDIQ"
     gene            complement(1693..3258)
                     /locus_tag="VF531_16430"
     CDS             complement(1693..3258)
                     /locus_tag="VF531_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7715605.1"
                     /translation="MPKNPWFLIILLIIALTGVGCGGGGGTNVNGLSGRVSFSGEPES
                     TMPLPSSGPVSMNQKGLIPQTVTMNSNLSASPSSLIVSLDPQLSEAEQRNLLAGLGFR
                     ILRRMLIDPNVYVVTPERSGITAAFDLAHNTPGIHKVEYNNKLFRESIVVPNDPNYGS
                     QWDMRLTKFDWIWDDYPGRDPVIVAVLDTGIIQNSSEFSERLTIVPGSDMVSDDDQPW
                     DDVYYNDACRYSHGTNVAGIIAASTGNSYGYAGAAGNAPIRIMPIRVLGPDNDGDIAT
                     IIDGIYLAVLNDASVINLSLGTDHAFDSDLFNHAVEYAASRDVLLVCAATSSGKRASY
                     SAVGPELTVIAPGGEVINGTTLQSVWNITFDKKGPQTEVLNYGMAGTSQATPHVSALA
                     ALLASYGLPAVEIKSLIATTSTDLGVEGRDDQYGFGMINAFAAFITAGGMDLSAITLG
                     LRDPVTHDDRIQPIHPDSSGNYRIPNPPQGQFEPYGIYPLDNGYQYFRTVPISITGPA
                     QTRNLILQLVPKN"
     gene            3611..>4062
                     /locus_tag="VF531_16435"
     CDS             3611..>4062
                     /locus_tag="VF531_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7715606.1"
                     /translation="MAQQYDVIIIGGGPAGIFAALELVRDPHLKIAIFEKGRGMSERT
                     CLAKEQQRACTKCNPCSVVCGWGGAGAFSDGKLTLSTEVGGFLDEYIPKQELSKLIRA
                     VDDTYLRFGAPTEVYGTDEEKIAQIEKRAVLADLRLIPSRIRHLGTGRS"
BASE COUNT         1009 a          973 c         1075 g         1005 t
ORIGIN      
        1 tggatttagc acatacgaca tggaattgta aataccatat agtttttgca ccgaagaacg
       61 ataatctacg gaaagatcaa gaaagacatt ggaacgatac taagaaagct atgtaaatac
      121 aagggtgtag agatagttga agcaaatgca tgtaaggatc atattcacat gttggcaagt
      181 ataccaccga aattgagcgt atcacaattt atggggcact taaaagggga aagctcactg
      241 ggtgccgaag gccggacatt ctcgcgatgc tcgaaaacat catcctgatc cgaaccgtct
      301 ctcgccatcc ccggctcaca accggttcag agtccgtcct tagaataaat aagctccaac
      361 ctatcggttg gagcttaata tgtttgaatt tggtgccgaa ggccggactc gaaccggcac
      421 gcttttaagg gcggcagatt ttgagtctgc tgcgtctgcc aattccgcca cttcggcagg
      481 tttacgtttg aaacgcacgg aactttagcg tgtagaatga ttttaccaca tgcaaaaatc
      541 aatttcaaga gggagatttt acaaatcccc gagaactgcc actccaaaat gacctatagt
      601 cttaatccgt ataggagaca tcctggaccg ggagttcctt gagtatagat tcaaagacag
      661 ttcgattcgc ctccgggatc accaggtgca gcgtgaccga tgcggtaaag ccggtatctg
      721 ccacggtggg gtcaacttgc tttagccggt ggaggatcaa ggaatgatcc gcataactgc
      781 aagtcagggt ggtgcaaaat gtgggtatcc tgacgcagga accggcagtt tttaggacca
      841 gggtggcggt ttggccgtaa gcatcgatca aaccccggac acctaatttt tttcctccga
      901 aataacgagt gaccactacc accacgttgg tcagcttgag ccgttcgatt gccccgagga
      961 tcggcttgcc cgcggtgccg ctcggttcgc cgtggtcatt atagtattcc aggggatctt
     1021 tcccggcgcc gagccggtag gcgtagcaat tatgattggc ctgagagttc tcagcgcgga
     1081 tttgcgcgat aaatgccttg gcctctgttt cacttgccac ctcccggcaa tggccgatga
     1141 agcgggagtg ttccacagtc aattcggcct tggccggggt ttgtacggtc aaataggatt
     1201 tctcattcat aagaatagta taccatgaag ctgcaagaga cggaacttat tggatatcca
     1261 gaccaaccgg ttgtccggtg attgccgggt tgccgcagaa taccggcttt aaaagataat
     1321 gtcccggctc aagcttaatt tgggaggcgt caatcaccgt gctatagatc tggggttcac
     1381 ccgctttgaa atcgcgtttc attaaactct gagcgtacat cttcatgtcg gagagctgcc
     1441 agatagtctt gccgtccttt tccaaaatca ggtcgtagag tttggcggag ctaaactcaa
     1501 tttgagtgtc cttcgttgta taaatcaata cagccacacc aataggagtg tttttccggt
     1561 aaacggtttg atcgagcgcc gccttgatcc ggatctccgg ttttttcgta gccattccaa
     1621 atccagcgat gagtgctaga gcgacaaggc tgataccaag aagataatag ttgcgtctca
     1681 tgaaaaccct ccttaatttt ttggtacgag ttgcaggata aggttccgag tctgggccgg
     1741 accggtgatg gagatgggaa cggtccggaa atattggtag ccgttgtcga gcggatatat
     1801 gccataaggc tcaaactgac cctggggcgg gttgggaatc cggtaattgc cgcttgaatc
     1861 gggatgaatc ggctgaatcc ggtcgtcatg ggtaacagga tcacgtaatc ccagtgtgat
     1921 ggcgctcaaa tccattccgc cggccgtgat aaacgccgca aaagcgttga tcatcccgaa
     1981 tccgtactgg tcatcccgcc cctcgactcc caggtcggtt gaggtggtgg cgatgagcga
     2041 cttgatctct accgcgggca gaccataaga agcaagcagc gccgccaagg cggagacatg
     2101 gggagtcgct tgggaggtac cggccatacc gtagtttagc acctcggttt ggggtccctt
     2161 cttatcaaag gtgatgttcc acacggactg aagtgttgtc ccgttaatca cctcaccacc
     2221 gggggcaatt accgtaagct ccgggcccac agcggagtat gatgcccgtt ttccggatga
     2281 ggtggcggcg cagaccaaaa gcacatccct gcttgccgca tactccacgg cgtgattgaa
     2341 taggtcgcta tcaaaggcgt gatcggttcc caggcttaaa ttgattacgc tggcatcgtt
     2401 caataccgcc aaatatattc catcgataat cgttgcgata tcgccgtcgt tatccggtcc
     2461 caggacccgg atgggcataa tccggatcgg cgcgttcccg gcggcaccgg cgtatccata
     2521 gctattaccg gtgctcgcgg cgatgattcc tgcgacgttg gtgccgtggc tgtagcggca
     2581 agcatcgtta taatatacat catcccacgg ttgatcatcg tcgcttacca tatccgatcc
     2641 ggggacgatt gtcaagcgtt cggaaaactc ggaactattc tgaatgatcc ccgtgtcgag
     2701 cactgcgacg atcaccggat cccggcccgg ataatcatcc caaatccagt cgaatttggt
     2761 cagccgcata tcccattggc ttccataatt cggatcgttc ggaaccacta tcgattcccg
     2821 gaagagttta ttattgtact ccactttatg aattcccgga gtattatgag ccaaatcaaa
     2881 agcggcggta atgcccgatc tctctggagt aactacatag acattgggat caatcaacat
     2941 ccggcgcagg atgcgaaaac ccaaaccagc caggaggttg cgctgctccg cctcggaaag
     3001 ttgtggatct aaacttacaa taaggcttga aggactcgcc gaaaggttgg aattcatggt
     3061 tacagtttgg ggaatcaatc ctttttgatt catggaaacc ggcccggaag agggcaaagg
     3121 catggtcgat tccggttcgc cgctgaagga tactcttccc gacaggccat tgacattggt
     3181 tccgccgcca ccgccgcaac caacgccagt gagggcgatg atgagcagaa tgatgagaaa
     3241 ccagggattt ttaggcatta ttaacctccg gtgccgagaa catggggatc ttagttgagg
     3301 ttgtcattat caattcggta actgctttaa gatctccttt tttggccggg ccatggttaa
     3361 caggtcgtga tctaaactat ccgacccagt attgatcgga actgcaaata ataaaggaga
     3421 tcactggaag atgttgaata gataaaagtt agttttcatg gggagacgaa catatttcca
     3481 ttttttccag gaaaactaag aaaggaatgt tttgtctcat gggtccggct ttgccgattg
     3541 aattcggccc caatgaaaag tagatgcttg atttgattca tttcatatca ttgaaacgta
     3601 ggtgacaaga ttggcgcaac aatatgatgt gattattatc ggtggcggtc cggccgggat
     3661 cttcgccgcg cttgagttgg ttcgtgatcc gcatttgaag atcgccatct ttgaaaaagg
     3721 ccgtggcatg tcggaacgaa cttgccttgc caaagagcag caaagggcct gtaccaaatg
     3781 taatccttgt tcggtcgttt gcggctgggg tggagccggt gcattcagcg atgggaaatt
     3841 gactctctcc accgaagtgg gcggcttttt ggatgaatac attcccaagc aggagttgag
     3901 caaattaatc cgggctgtgg atgataccta tttacgtttt ggcgccccca ccgaggttta
     3961 cgggaccgat gaggaaaaaa tcgcacagat cgagaaacgg gcggtactgg ctgatctacg
     4021 gctgatccct tcccggattc gccatttggg aaccgggcgt tc
//
LOCUS       DASVMB010000588         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_587889,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000588 DASVMB010000000
VERSION     DASVMB010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587889"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VF531_16440"
     CDS             <1..971
                     /locus_tag="VF531_16440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7715607.1"
                     /translation="NSLKLARLKLASAVEDERKAKQQLTYSVKEAYYRLWLAEKMQGV
                     AKSSYDNMERHYQRINSLYKAGTASRFDLLQAQVKWDNQKPSVIKAENGVKLARLSLA
                     TIIGIEPNREFQVEYDPSRLQLPEKVELSAQVVAEEAYQQRPELHQMQQLVEVAKANE
                     ALAKAGYLPNVALSYNYSLQGGSDIFLKNASNTVTLGVSLSGVAFDGFATPARVKSAS
                     ENLELTRINEVNIKNQIRLETEQALQSLEESLETIRANQANIDLAQESLKMTQARFAA
                     GMATTMDITDAQLVLDQASNGYYQGVSSYLTALAKLDLVAGKDQAQ"
     gene            complement(1121..1708)
                     /locus_tag="VF531_16445"
     CDS             complement(1121..1708)
                     /locus_tag="VF531_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7715608.1"
                     /translation="MLDKETKEVEVATVQGDKFYDLLAKTRAITISGEINQKLAEKVM
                     QQLLFLQELSDDPIKVFINSQGGHVESGDTIHDMFQFVKPRVIVIGTGWVASAAITIY
                     LGADKKDRYSLPNTRYLIHQPAGGVYGQAADIGIEAKELIKTRQRINRLISEATGQPL
                     EKVEHDTDRNFWMSASEAKEYGIVNNIISSLSEIR"
     gene            2124..3017
                     /locus_tag="VF531_16450"
     CDS             2124..3017
                     /locus_tag="VF531_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715609.1"
                     /translation="MKRITIFLLGVALVLYIKTVCFAAPAWEVSARQWESKGSFDYLL
                     FYPYSAQMESLVRFPQNQSLILVSLRHNLEDGRHFIYLEYGGSNPGLKGRGSDSDWTI
                     YGDDTLTYYGEMDGWGQVNRLTVEGGTLLARNGIWTVTLLGGWSRQETTNELKNIIYH
                     LIEGEDVGNQTQPDNGSRFDGVLSGYHFGIGNNLELNPRIHVNMKTALTILSARANGH
                     WANHDPAWDWVDTGNGLGFSGGLGMEYALGEKLLVNIRYDYFYANIPGCKEALNGEAL
                     EESVGLRYEQAGVFLGLNYRF"
BASE COUNT          798 a          735 c          837 g          780 t
ORIGIN      
        1 gtaattccct gaaacttgcc cggctaaaac tagcctccgc tgtggaggac gaacgcaagg
       61 cgaaacagca gttgacctac agtgtcaagg aagcatatta ccgtttatgg ctggccgaga
      121 agatgcaagg agtggccaaa agctcatacg acaatatgga acggcattac cagcggatca
      181 acagtctcta taaggccgga accgcttcgc gttttgatct gctgcaggct caagtgaaat
      241 gggataatca aaaaccgtcg gtcattaaag cagagaacgg tgtcaaactt gcccggctca
      301 gtctggcgac gataatcggg atcgagccca accgggagtt tcaagtggag tatgaccctt
      361 cccggttaca actgcccgaa aaagtcgaat tatcggccca agtcgtggcc gaagaggcat
      421 atcaacagag gccggaactt catcaaatgc aacaattggt cgaggttgcc aaggccaatg
      481 aggcattggc caaggccggg tatctgccga atgtggcctt gtcttataac tattcgttac
      541 aaggcggcag tgacatcttt ttaaaaaacg cctcgaacac ggttacgctt ggggtgagtc
      601 tgtccggcgt ggcgtttgac ggattcgcca ccccggccag ggtaaaatca gccagtgaaa
      661 atctggagct tacccggata aatgaggtca atatcaagaa tcagattcgc ctggaaacgg
      721 agcaagccct gcaaagcctg gaagaaagcc tggagactat ccgcgccaac caagccaata
      781 tcgatctggc ccaagagtcc ctcaaaatga cccaggcccg ttttgccgcc ggaatggcca
      841 ccaccatgga tatcaccgac gcccagctgg tgctggatca agcttcaaac ggttattatc
      901 agggcgtttc gtcctatctg accgctctgg ctaagctgga tctggttgcc ggaaaggatc
      961 aagcccaata gtccgggagt gaataaaata atggggcgta ccggtgaaat ttccggtacg
     1021 cccttgtttt atgcaaatac tttgattgca aaatatttaa taaagttggt atttctggcc
     1081 tattactaac ctctggaact ggacttcttt ttaaaagccc tcagcggatc tccgagaggg
     1141 aggagatgat gttattgacg attccgtact ccttcgcttc agaagcgctc atccagaaat
     1201 tgcggtccgt atcatgctcc accttttcca ggggctggcc ggtggcttcg ctgatgagcc
     1261 ggttgatgcg ttgccgcgtc ttaatcagct ctttggcctc aatgccgata tccgccgcct
     1321 ggccgtatac tcctcccgcc ggttgatgaa tcaggtacct ggtgttaggc agagaataac
     1381 gatccttttt atcggctccc agataaatcg tgatcgccgc gctggccacc cacccggtgc
     1441 cgatcacgat tacccgcggt ttgacaaatt gaaacatatc gtgaatggta tctccggatt
     1501 ccacatggcc gccctgggag ttaatgaata ctttgatcgg atcatccgaa agctcctgga
     1561 gaaataggag ttgctgcatg actttttcgg cgagtttctg gttgatctct cccgaaatcg
     1621 tgatggctct ggtcttggcc aggagatcat agaacttatc gccttgaacc gtagccactt
     1681 caacctcttt cgtttcttta tccaacatat cgaatcacct tcctggaact agtatatctt
     1741 ttttcggact tcaaatgcat attcgcgatg tttttccatt caccttccat ggatttcgtt
     1801 accgctcccc tttattcagg ttatttcaac tccggatggg aaaaaccgga tttattgtat
     1861 caggatgttg gttgaccatt gccattaaga gatttatgat attcaagagg caagaggcaa
     1921 gaggcaagag gcaagaggca agaggcaaga ggcaagaggc aagaggcaaa agaaagcaga
     1981 aagctcgcaa aacggtggtt tttgaaaggg tgtgcgccaa ctttgttggc gaggaagcct
     2041 tccgaagaag gcgcgccctg ccggggactg gcgtccggaa tggacgctct ggtgtcccca
     2101 gtgatctttc gaaaggatgg gcgatgaaac gaataacgat ttttctgctg ggagtggccc
     2161 tggtattata tatcaaaacc gtttgtttcg ccgcaccggc ctgggaggtt tccgcccggc
     2221 aatgggagag taagggttcc tttgattatc tgctctttta cccgtattcc gctcagatgg
     2281 aatcattggt gcgtttccct caaaaccagt cccttatttt ggtcagtctc cggcataacc
     2341 ttgaggatgg caggcacttc atctatctgg aatatggcgg ttcaaacccg gggcttaagg
     2401 gcaggggttc cgactccgac tggacgattt acggtgatga taccttaacc tactacggtg
     2461 agatggacgg atggggtcag gtcaaccgat tgaccgtcga aggaggaact ctcctggcgc
     2521 ggaacggtat ttggacagtc actcttttgg ggggatggag ccggcaggag acaaccaatg
     2581 agctgaaaaa tattatttac cacctgatcg agggggaaga tgtcgggaat caaacccaac
     2641 ctgataatgg cagcaggttt gacggagtat tgagcggata ccatttcggt attggcaata
     2701 atctggaact taacccgcga attcatgtaa atatgaagac cgctctaacc attttaagcg
     2761 cgagggcgaa cggtcattgg gccaatcatg acccggcctg ggactgggtt gataccggga
     2821 atggcttggg attttcgggg gggttgggaa tggagtacgc gcttggtgaa aaattattgg
     2881 ttaatatcag atatgattac ttctacgcca atatacccgg atgcaaggag gcactcaacg
     2941 gcgaagcgct agaggaatcg gtcgggcttc gttatgagca agccggggtt ttcctcgggc
     3001 tgaactatcg gttttaggtc gggatgttcc gcaggattat cggatcccat cgcaaatata
     3061 tatatattta tatatactga atcataatga ggttgtagaa agggcaccgg tagatgcaca
     3121 acgttaatga gattatcgct tatatcctcg
//
LOCUS       DASVMB010000589         6256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_734612,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000589 DASVMB010000000
VERSION     DASVMB010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734612"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1123
                     /gene="fabD"
                     /locus_tag="VF531_16455"
     CDS             191..1123
                     /gene="fabD"
                     /locus_tag="VF531_16455"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7715610.1"
                     /translation="MQKIAFIFPGQGSQYVGMGKDICAEYPVAKNVFDEADRVLGRSL
                     TALCFNGPEEDLKDTRNAQLCILATSVALYRVFEEAGFKPDFMAGHSLGEYSALAAGG
                     ALPFDTVLRLVDKRARLMAEADPEKQGTMAAVLGLTREAVFEILKQASEGGRVEAANF
                     NCPGQIVVSGAKNGIAKVQELVTAAGGKAIPLAVSGPFHSSLMRPAAEAIRDELAKAP
                     WREPAVPLIANISALPAHRDELAENLYHQMYSSVLWEDSLNYLSEQGVTVFMEVGPGK
                     VLSGLVKKTVKGVTILNCGDSDSVKKSLAILKEV"
     gene            1128..1865
                     /gene="fabG"
                     /locus_tag="VF531_16460"
     CDS             1128..1865
                     /gene="fabG"
                     /locus_tag="VF531_16460"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7715611.1"
                     /translation="MRLAREVALITGGARGIGKAIALTLVAEGASVVLSDVNDEVHQT
                     AAEIRESGGQAASIVGNVTKLEDCEAMVDEALKTFGKLDILVNNAGITRDNLLLKMTE
                     ADWDAVLDINLKGTFLCTKAAIKPMIKQRSGKIINIASVIGIMGNAGQANYAASKGGI
                     ISFTKTMARELGKRNIRVNAIAPGFIASKMTEVLSEEARNNLLEQIPLGSLGQPEYVA
                     QAVLFLASPEASYITGHVLNVDGGMAM"
     gene            1877..2146
                     /locus_tag="VF531_16465"
     CDS             1877..2146
                     /locus_tag="VF531_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7715612.1"
                     /translation="MGSIYTWKEVKGLDIFGKVKDIVVEQLGVDEEEVTEQASFVDDL
                     GADSLDIVELVMALEEEFDLEIPDEDAEKIVTVGDAVNYIKENAQ"
     gene            2410..3690
                     /gene="fabF"
                     /locus_tag="VF531_16470"
     CDS             2410..3690
                     /gene="fabF"
                     /locus_tag="VF531_16470"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7715613.1"
                     /translation="MRKSVLINSLGGGQVKHRAVITGMGVVSALGTSVPKFWESLVNG
                     VSGVDYVTGFDTTDFKVKIGAEVKDFDPEQYLDKKEVRRTDRVVQMAVGASLMALEDS
                     GLKIDSGNAPDIGVIIGSGIGGMKTFEEQARALLEKGPGRVSPFFIPMMIPDMSTGYV
                     SIVTGAKGPNHTVVTACASGTHSIGDALRIIERGDAQAMITGGSEAAITALAYAGFTS
                     AGALSTNNENPKGSSRPFDKNRDGFVMGEGAGVIILEELEHALKRGARIYAELVGFGE
                     SGDAYHMTSPDPEANGGARAMKLAIADAGITPEQISYINAHGTSTHQNDALETLAIKK
                     VFGDFASKVAVSSTKSMTGHLLGAAGGVETVISALALKHGIIPPTINYETPDPECDLD
                     YVPNHARKGDLQYILSNSLGFGGHNACVVLKKYV"
     gene            3702..4421
                     /gene="rnc"
                     /locus_tag="VF531_16475"
     CDS             3702..4421
                     /gene="rnc"
                     /locus_tag="VF531_16475"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7715614.1"
                     /translation="MDRISHHDRNTEKFEPPIGELAAKLHFEPTSEGLFVEAFTHSSY
                     ANEHRIASNERLEFLGDSVMGLVVCEFLYRKFPDSEEGKLSKLKSILVSAGVLASFSK
                     QMGLDPYLRLGQGELKTNGRGKLNILADLFEAFVGAYFLNFGFEATRGFILPLIRQAL
                     PEVVAETKLIDAKSSLQELLQARGLKPEYRTISESGPPHDRWFTVEVYCNEKLLGTGT
                     GRTLKEAQNQAARVGMERIKS"
     gene            4516..4776
                     /locus_tag="VF531_16480"
     CDS             4516..4776
                     /locus_tag="VF531_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEX7715615.1"
                     /translation="MDVLKVSANSSPKSVAGALAAVLREKGIAEVQAVGAGAVNQAVK
                     AIAITRGYVAPNGINLICTPGFSEITIDNQVRTAIKFIVEPR"
     gene            4906..5631
                     /locus_tag="VF531_16485"
     CDS             4906..5631
                     /locus_tag="VF531_16485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7715616.1"
                     /translation="MKTTGATRVKVVEGGACARNQENDLALLRRIRTERDLDCREEMV
                     KKYLPMVRHIVKHQNVPWPDFDDYIQEGTIGLLKAIDEYDSEHYPIKFSTFAYICITR
                     RIYNALKQAYGRKILPSNQIISLNSFLNEEDSRTLIDSIPDLSSEPSTQIEEALVGQY
                     LKTVLEAYLSPVEYRVVQLIWNGYNLNEIHCTLALSHKVIDNARTRARLKLRKVLFEY
                     GSLSDPRIPMKTRKRKDLAIKVV"
     gene            5937..>6256
                     /locus_tag="VF531_16490"
     CDS             5937..>6256
                     /locus_tag="VF531_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7715617.1"
                     /translation="MYLKRLEIVGFKSFADKIRLDFLPGVTGVVGPNGSGKSNISDSL
                     RWVLGEQSIKNLRGMKMDDVNFAGSQLRKPLGLAEVTIILDNSDHSIPIDFSEISVTR
                     KLFRS"
BASE COUNT         1570 a         1412 c         1701 g         1573 t
ORIGIN      
        1 cgtgccgtta ctcggtgtga atggattgag tttgatcggc catggccgct ccaatgccaa
       61 ggctatcaaa agtgcgattc gcgcggcaat gagagccgca gatcaagatc tggtcggaaa
      121 aatagcatct gcggtaaagt aaggacgaga tatgcgcgca cacgcgtcgt ccaaattttg
      181 aggagtctcc atgcaaaaaa ttgcgtttat cttccccggt caaggttcgc agtatgtagg
      241 tatgggtaag gatatttgcg ccgaatatcc ggtggcaaaa aatgtttttg atgaagcgga
      301 ccgggtcttg ggtcgttcgt tgacagcgct ttgtttcaac ggccccgagg aagacctgaa
      361 ggatacgcgg aatgcccaat tatgcattct tgcgactagc gttgccttgt atcgggtgtt
      421 tgaggaagcg ggattcaaac ccgactttat ggcaggtcat agtttgggag agtatagcgc
      481 cctggcggcc ggcggagcct tgccatttga tacggtattg cgactggtgg ataaaagagc
      541 ccggttaatg gctgaggccg atcccgagaa acagggaacg atggccgccg ttcttgggct
      601 cacgcgtgaa gcggtttttg agattttgaa gcaggcttcg gagggtggcc gggtcgaggc
      661 tgccaatttc aactgtcccg gccagattgt ggtctccgga gccaaaaacg gtatcgccaa
      721 ggttcaggag ttggtgactg ctgccggtgg aaaagcaata ccgctggcag ttagtggccc
      781 gttccattcc agtttgatgc gcccggccgc agaagccatc cgggatgaac tggccaaggc
      841 cccttggcgg gaacctgcag ttccgctcat cgctaatatt tcagctttac cggctcatcg
      901 ggatgaatta gcggagaatc tctaccatca gatgtattcc tccgtcttgt gggaggatag
      961 tctgaattac ttatctgaac aaggtgtcac tgtcttcatg gaggtcggtc cgggaaaagt
     1021 tctatccgga ctcgtaaaaa agactgtaaa aggcgtaacc attctaaatt gtggagattc
     1081 ggactccgtt aaaaaatcac ttgcaatttt aaaggaggtc tagtaatatg agattagcgc
     1141 gcgaggttgc cctcattacc ggcggcgcga gaggtatcgg caaggcgatt gcccttactt
     1201 tggttgccga aggcgcatcc gtggtactct ccgatgtcaa cgatgaagtt catcaaactg
     1261 ccgcggagat tcgtgagtcc ggaggacaag ccgcttcgat tgtggggaac gtcaccaagc
     1321 tcgaagattg cgaagcaatg gtggatgaag cgcttaaaac ctttggaaaa ttggatattc
     1381 tggtcaataa tgcggggatc accagagata atctgttact caaaatgacc gaggccgatt
     1441 gggatgcagt cctcgacatt aacctcaaag gaacatttct ttgtaccaag gcggcgatta
     1501 agccgatgat caaacaacgt tccggcaaga tcatcaatat cgcctcggta atcgggatta
     1561 tggggaatgc gggtcaggcg aattacgcgg cctccaaggg tggtatcatt tctttcacca
     1621 aaaccatggc ccgggaactg ggtaaaagaa atatccgggt gaacgccatt gctcccggtt
     1681 ttatcgcctc caaaatgacg gaggtcttat ccgaagaagc ccggaataat ctgttagaac
     1741 agattccctt gggaagtttg ggtcaaccgg agtatgtggc ccaggcagtt ttatttttag
     1801 catctccgga agccagctac atcacaggtc acgttctgaa tgttgatggc ggtatggcaa
     1861 tgtaagttct cttcatatgg gatcgattta cacttggaag gaggtgaaag ggttggatat
     1921 cttcggtaaa gtcaaggaca ttgtagttga gcaattggga gtggatgaag aagaagttac
     1981 cgaacaggct tcttttgtag atgatttggg cgccgattcc ttggatatcg tagagttggt
     2041 catggcgctt gaagaggagt tcgacctgga aataccggat gaggacgccg aaaagatcgt
     2101 gactgtcggc gacgcggtaa attacatcaa agagaacgca cagtaagacc atagaaagtc
     2161 ccgtagatac ttacgggact tttttaacct cgatttggtg agagtggttt gatgaaaaac
     2221 cggaatattc gccgaatcgc agtggaatca cggtttacaa aatgtgaaaa ttcagctttt
     2281 aattctaaag atacaataga aacggatttt ttttatacct caggttttta tcccactccg
     2341 atttgagctt cccagggcag atgaaagccc tcactgtttt cgtcaaatat gtttcaaacg
     2401 agtatttcca tgcgcaaaag cgtattaatt aattctttag gtggtggcca ggtgaaacat
     2461 cgtgcagtaa ttaccggtat gggtgtcgtt tcggccttag ggaccagtgt tcctaagttt
     2521 tgggaatcat tagtcaatgg agtttccgga gtcgactacg ttaccggatt cgataccacc
     2581 gattttaagg tgaagattgg ggccgaggtc aaggattttg accccgagca atatttggat
     2641 aaaaaagaag tgcgtcgtac ggatcgcgtg gttcaaatgg ccgtcggcgc ttccttaatg
     2701 gcgcttgagg attcggggct gaaaatcgat tccggtaatg caccggacat cggggtcatt
     2761 atcggctcgg gaatcggcgg aatgaaaacc tttgaagagc aagcgagggc tttattggaa
     2821 aaggggccgg ggcgggtgag cccgttcttt attccgatga tgatccccga tatgagcacc
     2881 ggatacgtat cgatcgttac cggagcgaaa ggtcccaacc acacggtggt gaccgcctgc
     2941 gcctcgggaa cccactccat cggggatgcc ctccgcatca tcgagcgggg tgacgctcag
     3001 gcaatgatta ccggcggcag tgaggcggcg attaccgctt tggcctatgc cggatttacc
     3061 tccgccggcg cactttctac caataatgag aaccccaaag ggtccagccg cccatttgat
     3121 aaaaaccggg acgggtttgt catgggcgag ggcgccgggg taatcattct cgaagagctg
     3181 gaacacgccc tcaaacgggg cgcccggatc tacgccgaat tggtcggatt tggggagagt
     3241 ggcgatgctt atcatatgac atcgcccgat ccggaagcca acggcggagc ccgtgccatg
     3301 aaactggcca tcgccgatgc cgggattaca ccggagcaga tttcatatat caacgctcac
     3361 gggacatcca cccatcagaa tgatgccctg gaaaccctgg ccatcaagaa ggtttttggt
     3421 gattttgcat ccaaggtggc tgtaagctca accaaatcta tgaccggtca cttactgggt
     3481 gcggccggag gtgtggagac cgtaatttcg gcattagctc tcaagcatgg tatcattcct
     3541 ccaaccatta attacgagac gccggatccc gaatgtgatc ttgattatgt ccccaatcac
     3601 gcccggaaag gcgatctcca gtatattctc tccaactcat tgggttttgg cggacataac
     3661 gcctgtgtcg ttctgaaaaa atacgtctag gagactgaat cgtggaccgc atcagccatc
     3721 atgatcggaa caccgagaag ttcgagccgc caattggtga attggcggct aaattgcatt
     3781 ttgaacccac gagtgagggg ctttttgtcg aggcatttac tcattcgtcg tatgccaacg
     3841 aacaccgcat tgccagtaac gaaaggttgg agttcctcgg ggacagcgtg atgggtctcg
     3901 tcgtatgcga gtttctttac aggaaatttc cagattcaga ggaagggaaa ctctccaagc
     3961 tcaaatcgat tctggtgagc gccggggttc tggcctcatt ctccaaacaa atgggacttg
     4021 atccttacct ccgccttggc cagggagaat tgaaaacgaa cggccggggt aaactcaaca
     4081 ttttagccga tctatttgag gctttcgtcg gagcgtactt tttaaacttc gggtttgagg
     4141 ctacccgagg tttcatatta cccttaatcc ggcaggcctt gccggaggta gttgcggaaa
     4201 ccaagttgat cgatgcgaaa agcagcctcc aggaattgct tcaggccagg ggtttgaaac
     4261 ccgaataccg caccatctcc gaaagcggac cgccgcacga ccgttggttc acggtggagg
     4321 tttactgcaa tgagaaactc ttggggaccg gaacggggcg aaccctaaag gaagcccaaa
     4381 atcaggcggc ccgggtcggg atggaacgga ttaaatcgta ggcgaactgc cgcgattttt
     4441 tttatctggg ttttttttgg gtgattgttg aatatgatag ggaggaagca acattctgca
     4501 ataggaggct ttctcatgga cgtattaaaa gtttccgcaa attctagccc caaatcggtt
     4561 gctggagcct tagccgctgt gctgcgtgaa aagggcatcg ctgaagtgca ggctgtggga
     4621 gcgggcgcag tcaatcaagc tgttaaggcg attgcaatta cccgtggtta tgtagcaccc
     4681 aacggaatca atcttatttg tacccccgga ttctccgaga ttaccatcga taaccaagtt
     4741 cgtactgcca tcaagtttat cgtggaaccg cgctaaacgc attatccata catgagagaa
     4801 taaaagaccg ctaaccaagc ggtttttgtt tttgaaaagt ttcgtcatat cccggacaac
     4861 ctggccatat atttatttta agagagatta aggaggttga acgggatgaa aaccaccggg
     4921 gcgactcggg tcaaagtcgt ggaaggcggc gcgtgcgcgc gcaatcagga aaacgatctc
     4981 gctttgctcc ggcgcattcg taccgaacgc gatttggatt gtcgcgaaga aatggtcaag
     5041 aaatacctgc cgatggtcag acatatcgtc aaacaccaga atgttccctg gccggatttt
     5101 gatgattata tccaggaggg aaccattggg ttattgaagg cgattgatga atacgattcg
     5161 gaacattatc ccatcaagtt tagtaccttt gcctatatct gcatcacccg ccggatctat
     5221 aatgccctta aacaggcata tggccgaaaa attttaccca gtaaccagat catctcattg
     5281 aatagttttc tgaatgagga agattcccga accctgatcg attcgattcc ggatctcagt
     5341 tccgagcctt cgacccagat agaagaggct ttggtagggc agtacctcaa aacggtgctc
     5401 gaagcctatt tatctccggt ggagtaccgg gtggtccaac tcatttggaa cggttacaat
     5461 ctgaatgaga tccattgtac cctggcgctc tcccataagg tgattgataa cgcccggacg
     5521 agggcgcgtt tgaaactaag aaaggtttta tttgagtacg gttcgctttc ggatccccga
     5581 attccgatga agacccggaa acggaaggat ttggccataa aggtagtata atgcgggtag
     5641 ataggcttca cgccggatcc ctcgaatgtc ttttcatcct gattcaacca gcgactgata
     5701 atcaataacc aataacgaat tacttcattt ccccagctac aggcccgata cgtaaatgtg
     5761 tcgggtattt cttttttatc cgggttatgg taaaatgttt gatactgagt cggtttgccc
     5821 gcatagggat tcaaccggtc gttatttttc cttccgccaa atctccgcat tttgattcga
     5881 atagtattgg actgggtgtg acttcgggcg agagccctga gttaaggggg atccatttgt
     5941 atcttaaacg tttggagatt gttgggttta aatcatttgc cgataagatt cgccttgatt
     6001 ttcttccggg ggttaccggg gtagtcggtc ctaacggcag tggtaaaagc aatatatcgg
     6061 actcgcttcg ctgggtgctg ggtgagcaga gcattaaaaa tctgcgcggg atgaagatgg
     6121 atgatgtgaa ctttgccggg agccagctcc gaaaacctct gggtttagcg gaggtcacca
     6181 ttatcctgga taactccgac cactccatcc ccattgattt ttcggagata tccgttaccc
     6241 ggaagttgtt tcgttc
//
LOCUS       DASVMB010000590         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_735074,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000590 DASVMB010000000
VERSION     DASVMB010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735074"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1999)
                     /locus_tag="VF531_16495"
     CDS             complement(215..1999)
                     /locus_tag="VF531_16495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7715618.1"
                     /translation="MNRFNSPRTLKSKIIIYFLLITVVPSVITSYFYYRVSQNNLEKS
                     MRETSISDLVYSMDIIDRQLINAGQLSDWIFMNRNLDDILTRRTINRYSPEIKSFLDL
                     IDYQLRFNTAVSNYVSSFIIVGKNGLDLRAGPPEGSLIDIGRVEKTDWFQKGLDQHGL
                     KSWYGVVANPATINYDAYILPLVRPVLHSFTNREIGWHMIGFKTALISDLFKNLEIRR
                     DESLLVLDSRGFCIYHNDPKYIGRDLSNLDYIRTIVKRNREGYIKSRINGVNRLVAYA
                     RSSQTGWSIIRVLSEAELSRQKRMLFNISLVILLSSLFFTSLLTFYLSTNLTNPLTKI
                     SRKTKAIAAGDFAPDPAIEGRDELGTLGRAINEMASNIKGLLDRLIADEQEKRRMEID
                     LLQHQVNPHFLYNTLNSLKLMATIQGADGIKDMVSALGRLMMNLSKNTSEKIPLAEEF
                     ALLNDYVYIQNIRYKGKIKLEYHLEDEACQKNKIIKFTLQPIVENAIFHGIEPKKDAG
                     TIKIAVRAAEDHLNVSVTDDGVGISPEQIEAVLTKPPEGKTRGLSGIGIKNVAERLKL
                     VYGPEYGLTIESKVGEYTRVELKIPREL"
     gene            2467..4263
                     /locus_tag="VF531_16500"
     CDS             2467..4263
                     /locus_tag="VF531_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7715619.1"
                     /translation="MAEVQRAHTAEKSDIFLANIHDNDYNQTYLAKPITHESILFDRN
                     RKKESLNGAWSFGIDQYDTCIRAKWYEEKYRDPDGRQLPVDFTFDRWEKIPVPSCWNL
                     QKERYFLYEGSAVYTRTFEYRNHGEKRVFLKFGAVNYEAKVFLNQKYLGMHRGGSTPF
                     YIDITPELLPTNRLLVVVNNTRKQEAVPADNTDWFNYGGIYRDVELIRLSETFIKEFN
                     VALVPGSSAQQIRIRLVIAGPDGEGTAALKIPELNLTAVIPVHSGKGEAVINAAPRLW
                     SPEDPKLYEVTLSYLEDRVTDKIGFREIQVVGTEILLNSKPILLKGICSHEESASSGK
                     AVSDAEIIENIKLAKELNCNYMRLAHYPHSARVAELADELGIMLWEEIPVYWAIDFTN
                     PETYANAENQLTELIKRDQNWASVIIWSVGNENADTDPRFKFMSSLAHKAKELDPSRM
                     VSAACLVDGVNLRIADRLAETLDVIGINEYYGWYEPDFTKLIKIFENSKPTKPVIISE
                     FGADGRLGARGTADDLGTEDCQLAIYRKQLDTIGKIPYVKGTSPWILYDFRCPRRLHE
                     LQDYYNIKGLLSADKRHRKLAFYAVRDFYEKR"
     gene            complement(4312..5301)
                     /gene="mgrA"
                     /locus_tag="VF531_16505"
     CDS             complement(4312..5301)
                     /gene="mgrA"
                     /locus_tag="VF531_16505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX7715620.1"
                     /translation="MSYLPESNRYTTMKYNRCGKSGLKLPAVSLGLWHNFGGVTLFEN
                     SRTMVRKAFDLGITHFDLANNYGPPPGAAEENFGRILHQDFRAYRDEMIISTKAGYTM
                     WEGPYGDWGSKKYLLASLDQSLKRMGLDYVDIFYHHRPDPETPLEETMAALDLAVRQG
                     KALYVGISNYKPAEAKEAIRILRQLGTPCLIHQPSYSMFNRWVEDGLLDLLEAEGVGS
                     IAFSPLAKGLLTARYLNGIPADSRAASSSVFLKPEDVTPEKIGKVRQLNELAASRGQS
                     LAQMALAWVLRGGRVTSVLIGASKPSQIEDCAGTIQNLEFTREELDKIEAILK"
BASE COUNT         1501 a         1430 c         1323 g         1445 t
ORIGIN      
        1 aaaacgggct gggaagaact ggatgtatta cccagcccca ttcatggggc ttgagatgat
       61 ttttccgggc gtagccaggt cctttagggc ctggtgggga taaggacgat tatccattgt
      121 ttgcttttga gacagccccg acgggggtga gttgcttttg tgctttcagc cttggacctt
      181 aagcttcctc tcgcctcgcg cctcagcctt ttacctatag ctccctcggt atcttcaact
      241 caaccctggt atactcgcca accttgcttt cgatggttaa cccatattcc ggaccataaa
      301 ccagtttaag ccgttcggcg acattcttga ttccgattcc tgataacccc cgggtcttgc
      361 cctccggtgg cttggtcagg accgcctcga tctgttcagg ggagatcccg accccatcgt
      421 ccgtaacgct gacattcagg tgatcctccg ccgcccgaac ggctatctta atcgtgccgg
      481 catccttttt gggctcaatc ccgtgaaaaa tggcgttttc cacaatcggc tgcaaagtaa
      541 acttgatgat cttatttttc tggcaggcct catcctccaa atgatactcc aatttgatct
      601 tgcctttgta acggatgttc tggatgtaaa cataatcatt gagcaaagca aactcctcgg
      661 ccagagggat cttttccgaa gtgttcttgg aaaggttcat catcaatcgt cccagagccg
      721 aaaccatatc cttaatgccg tccgcccctt gaatggtggc cattaattta agggaattca
      781 gtgtattata taaaaaatga gggttcacct gatgttgcag tagatctatc tccatccgcc
      841 gcttttcttg ctcatcggca atcaacctgt ccaatagtcc tttgatattg gaagccatct
      901 cattaatggc ccgtcccagg gtacccagct cgtcccggcc ttcaatggcc gggtcgggag
      961 caaagtcacc ggcggcaatg gcctttgttt tccgggagat cttggtcagg ggattggtta
     1021 ggttggtcga gagataaaag gtcaataaag aagtgaaaaa caagctgctt aacaaaatta
     1081 ccaggctgat attaaagagc atcctttttt ggcggcttaa ttcggcctca gacaaaaccc
     1141 ggataatact ccagccggtc tggcttgatc tggcgtaagc cactagtctg ttaacgccgt
     1201 taatacggct tttgatataa ccctcccgat tccgcttgac gatcgtccgg atataatcga
     1261 gattacttag atcccgcccg atatatttcg gatcgttatg atagatacaa aaacccctag
     1321 aatccaagac gagcaggctt tcatcccttc tgatttcgag atttttgaag agatcggaga
     1381 tcagcgcggt cttaaagccg atcatatgcc agccgatctc tcgattggtg aaggaatgaa
     1441 gcaccggacg gaccaggggt aaaatatagg catcataatt aatggtggcg ggattggcga
     1501 ctactccata ccaggacttt aaaccatgct gatccaggcc tttttggaac caatcggttt
     1561 tttctaccct cccgatatca atgagcgacc cttcgggcgg tcccgcccga agatcaaggc
     1621 cattcttgcc gacgatgata aatgatgaaa cataatttga aaccgcggta ttaaaccgta
     1681 attggtagtc gatgagatca agaaaggact taatctccgg gctgtatcgg ttaatagttc
     1741 tccgggtcaa tatgtcatcg agattccggt tcataaagat ccaatccgag agttgaccgg
     1801 cgttgataag ttgccggtcg atgatatcca tggagtaaac caaatcactg atggaagtct
     1861 cccgcatgct cttttccagg ttgttttgcg agactctgta atagaaataa ctggtgatca
     1921 ccgaaggaac tacggtaatc aacaagaagt aaatgatgat cttgcttttg agcgtcctgg
     1981 gggaattaaa tctgttcatg gcatctccta tcccgtgtct cccgggtgat atctcacgcc
     2041 agcttgagtg ttgtacccaa ttacacctca taggaacaag tacaggtatc gcctctatag
     2101 taccgcttca gtaaattaca ttcaagggag atatctcccc atcaaaggaa tcccagacct
     2161 ttcaaggctt tttacaaaac catccgctac ttaatagtca tcaagaatga tccgcacctg
     2221 gaaatctgag attaaacgcc cctctcatca aaaccttgcg ccctcaaaac cgaacaaatg
     2281 aaaatacaaa ttatcgtaag aaatcccatt tttatttgag cataattccc ttataattga
     2341 actgtgtttt taaattgggg tagactattg atctgaaaac cttttcgctt aagcaatggt
     2401 ttatccatca ttccctaaac cggctgtgaa agtcgtttca caactactca aaggaggagt
     2461 taagtgatgg cggaggttca gcgcgcgcat acggcagaaa aatccgatat ttttttagcc
     2521 aatattcatg ataatgatta caaccaaacc tatttggcca aaccgatcac ccatgaatca
     2581 atccttttcg accgtaaccg gaagaaggaa tccctgaacg gagcgtggag ttttggaatc
     2641 gaccaatacg atacttgcat ccgcgccaaa tggtatgaag agaaatatcg tgatcccgac
     2701 ggccggcagc ttccggtgga cttcaccttt gatcgctggg aaaagatccc ggtaccgtcc
     2761 tgttggaacc tgcaaaaaga acgctacttt ctatatgaag gcagcgccgt ctatacccga
     2821 acattcgaat atcggaatca cggtgaaaaa agagtcttct tgaaattcgg cgctgtgaat
     2881 tatgaggcca aagtcttcct caaccagaaa tacctcggaa tgcaccgggg cggttcgaca
     2941 ccgttttata tcgatatcac ccctgagctt ttacccacca accggctcct ggttgtggtc
     3001 aacaataccc ggaagcaaga agcagtaccc gccgataata ccgattggtt caactacggg
     3061 ggcatctatc gggacgtgga gctgatccgg ctatcggaaa cctttatcaa ggagtttaat
     3121 gtggctctgg ttccgggcag tagtgcgcaa caaatccgga tccgtctggt aattgccggt
     3181 cccgatgggg aaggtaccgc ggcactaaag attcccgagc tgaatctcac agcagtcatt
     3241 ccggttcact ccggtaaagg tgaagcggtt ataaatgccg caccccgcct ctggtccccg
     3301 gaggatccca aactttatga agtaaccctt tcctacctgg aggatcgggt tacagacaaa
     3361 atcggttttc gtgagattca agtcgttgga accgagattc ttctcaacag caaaccgatc
     3421 ctgctgaaag gcatctgcag ccatgaagaa agcgcctcga gtggaaaagc cgtctccgac
     3481 gcggagatca tcgaaaatat caaacttgcc aaagagttga actgtaacta tatgagactg
     3541 gctcattatc cccattccgc ccgggtggcg gaactcgccg atgaactcgg gatcatgctc
     3601 tgggaggaga tcccggtata ctgggcgatt gattttacca atccggaaac atacgccaat
     3661 gccgaaaacc aattaacgga attgatcaaa cgggatcaaa actgggccag cgtcattatc
     3721 tggtcggtgg ggaatgaaaa cgccgatacc gacccgcgct tcaagttcat gagctccctg
     3781 gcacacaagg ccaaagaact tgatcccagc cggatggttt ccgctgcttg cctggttgat
     3841 ggagtcaatc tgcggatcgc cgatcgcctg gccgaaacct tggatgtcat cgggattaat
     3901 gagtattacg gttggtatga gccggacttt acaaagctaa tcaagatctt tgaaaacagt
     3961 aagccaacga aaccggtgat tatctcggag tttggagccg acggccgact gggagcgcga
     4021 ggtacagcgg acgatctggg gacagaggac tgtcaactgg cgatctaccg gaagcaactg
     4081 gacaccatcg gcaagatccc ttatgtcaaa ggaaccagcc cctggatact ctatgatttt
     4141 cgatgcccgc gccgcctcca tgaattacag gattattata atattaaagg actgctttca
     4201 gcggataaac gccaccgtaa acttgcattc tatgcggtac gcgattttta cgaaaaacgt
     4261 taatttaaaa taaatacccc cggcagtgcc gggggtattt atttttacag gttatttcag
     4321 gatcgcttca atcttatcca actcttccct ggtgaattcg aggttctgga tggtaccggc
     4381 acaatcctca atctggctgg gcttactggc gccgatcaat accgacgtca ccctgccgcc
     4441 ccgcagcacc caagcgagag ccatttgggc caggctctgt ccccgggaag cggcaagctc
     4501 gttcaattgc cggactttcc caatcttctc gggagtgaca tcctccggtt tcaagaaaac
     4561 actggaactg gcggctctcg agtcggccgg aatcccattt aaatatctgg cggtgagcaa
     4621 tcccttggct aacggcgaaa aggcgatact gcccactccc tccgcttcga gcagatcgag
     4681 taaaccgtcc tccacccaac ggttgaacat cgagtaggaa ggctggtgaa tcaggcaggg
     4741 agtacccagt tgccttaaga tacggatcgc ttccttagcc tccgccggtt tataattgga
     4801 aataccgaca tagagcgctt tcccttgacg aaccgccaag tctagcgcag ccatggtctc
     4861 ctccagaggc gtctcgggat ccggccggtg atggtagaag atatccacgt aatcgaggcc
     4921 catccgtttt aggctctggt cgaggctggc aaggagatat ttcttggaac cccaatcgcc
     4981 gtaaggacct tcccacatgg tataaccggc cttggtcgag atgatcatct catcccggta
     5041 agccctaaaa tcctggtgta agatcctgcc gaagttttcc tccgccgcgc cgggaggcgg
     5101 accgtaatta ttggccagat caaaatgggt gatgcccaaa tcgaaagcct ttcgcaccat
     5161 agtccggctg ttttcaaaga gtgtgacccc gccgaaatta tgccatagcc ccagggaaac
     5221 ggcgggaagt tttaagccgc ttttaccgca ccgattatac ttcatggttg tatagcggtt
     5281 actttccggt aaataactca ttgttcaacc tcctatcaag tgatgaccag taaacgaaga
     5341 ttaaagccac ataatatttt atcatatctt cctttcatcc gcccaaaaag taccctactc
     5401 atattaatat agttatcagg agtatccgct aataaaaaga taaactttct tcaaaatcta
     5461 atctttccat tgcctgggat atgttattct atatatgaga atatgtaaga acagtcggga
     5521 agagtataac cccgaaatac ctcattcact tctatcggag gttgacgtaa tgacagaata
     5581 ttgggaaaaa ctacaaaatg gtagtgacat tcgcggtatt gccctaccag gcattgaggg
     5641 agaggcagtt aatttaactc ccgaagcggt tcaggccatt agctcagctt tcggatcct
//
LOCUS       DASVMB010000591         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_735142,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000591 DASVMB010000000
VERSION     DASVMB010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735142"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VF531_16510"
     CDS             <1..364
                     /locus_tag="VF531_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715621.1"
                     /translation="NPVYTEDGAIRKVFTRNDYHGTVVWSWQQAMLAAGLRKQLQRTD
                     LTSAVRGSLHAAEKEVWKVINATKLNAASELWSWTYDNGRYRAVPFGQSNRDNAESNA
                     AQLWSTVYLAVKEPQEVP"
     gene            554..1366
                     /locus_tag="VF531_16515"
     CDS             554..1366
                     /locus_tag="VF531_16515"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7715622.1"
                     /translation="MFRIGEFASLNRVSTKLLRHYDAIGLLKPEQIDTDSGYRYYSAT
                     QIAVLNRIMALKDLGFSQTEISGLLKENLSAEEMIGILKSRQNQIRKAIAAENERLGR
                     IDRLVILFKQEVGMMNYDIVVKPVEGFRVASLRGIIGHYGEQGPLWEKLGEHLRKHHA
                     KIMPGCFVTYYGEIEGRGIDAEVVEPIDRDIPAGDGIKVELLPDVEKMAAVVHKGPYE
                     SLNIAYAAITRWMEENGYRITGQVREIYLKGDWDSDNSNEYVTEIQLPVTKD"
     gene            complement(1489..2877)
                     /locus_tag="VF531_16520"
     CDS             complement(1489..2877)
                     /locus_tag="VF531_16520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7715623.1"
                     /translation="MIREFFGDREFYRIFFNLAVPVTLQYFISASLNLIDGIMVGQLG
                     ATELAAVGLANQVTFMLLLFLLGVAGGTSIFVAQFWGRRDIQNIRRVLGLGTIISVTV
                     ALVFSLVNICCGPAIIKIFSDDPEVVALGGRFLSITSLAYVLMALSACYAAALRSTGE
                     VKLPMRANILAIVTNTGLNFLLIFGNFGLPGLGVTGAAVATVIARFLETTILLLSAYR
                     NRSAVAARFSEMFDIPADLVKNFISSTGTVVAKDLIWAIGVTVYMVVYARMGTDVVAS
                     INIINTVRQLAFVLFNGIAAAAMVMVGNQIGSGDEAKAFRYAQWFLTLTLLIGVVMGV
                     LLITGSRVILSPYKVSGAVIQGAKTVLFVTALYMPAIVFNMVAVVGVLRSGGDTMFCL
                     IMDLVAVYLIGLPLAIVGQAVWKLPVAGVYALITSQEIFKMILCLKRFGSKRWIHNLV
                     DGLNYHRAVEVK"
     gene            complement(2892..>3509)
                     /locus_tag="VF531_16525"
     CDS             complement(2892..>3509)
                     /locus_tag="VF531_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715624.1"
                     /translation="KKVVITSGLLNVLAGKGIDGIAPVRVSDRKAMVGQFGYPMYECS
                     YGNYYDAPAPFLIPQVEYSTNDCIPLIAAMAEKKSYPLLLQVRYGRGILYILTIPDHP
                     GDMYHLPEPVLNQIRRTLSQELPVRLEAPANVGLFVYANRTLIVESFLPHNTDVKIAV
                     TGTGLELVDLINGNHIPGNNNQGETLFDMKLSPGTYRVLRYQNKE"
BASE COUNT          936 a          860 c          931 g          782 t
ORIGIN      
        1 caacccggtt tatacggagg atggggcgat ccgaaaggtt tttacccgga acgattacca
       61 cgggaccgtg gtctggtcct ggcaacaggc aatgttggcg gcgggactcc gaaaacaact
      121 gcaaagaacc gacctgactt ccgcggtacg cggaagtctg cacgctgcag aaaaagaagt
      181 atggaaagtt atcaacgcca caaaattgaa tgctgcctcg gaactctgga gttggaccta
      241 tgataacggc agatatcgcg cggtcccctt cggacagagc aacagagaca atgcggaatc
      301 caatgccgcc caattatgga gtacggttta cctggcggtg aaggaacctc aagaagtacc
      361 ttaatagaca taaagaaaca agcccaaaaa aggccggata tccggccttt tttggttagg
      421 gtcgccatca actttcaaaa taatctgtaa aaaacttgat ttgccccttg actttcctgt
      481 tggggtaagg tttatgctag tcatgaagtc tgcgcgcgcg cgaatcatga aagtgcgcaa
      541 ctggagtgat tcgatgttcc gaatcggtga gtttgccagt ttaaaccggg tttcgaccaa
      601 gctgctccgg cattatgatg ccattgggtt gctaaagccg gaacagattg atacggatag
      661 cggctaccgc tactattcgg cgacgcagat tgctgtcctc aaccgaatta tggccttaaa
      721 ggatctgggc ttttcacaga cggagatctc cggtttatta aaggagaacc tgtcggctga
      781 agagatgatc ggtatcctca agtctcgcca gaaccagatt cgaaaagcca tcgccgccga
      841 gaatgagaga ctggggcgaa ttgacagact cgttatccta tttaaacagg aggtcggaat
      901 gatgaattat gatatcgttg tgaaaccggt tgagggtttc agggttgcgt ccttacgggg
      961 aattatcggg cattatggtg aacaggggcc tttatgggag aaacttggag aacatctccg
     1021 caaacatcat gccaagatca tgcccggttg ttttgtaacg tactatggag agatcgaagg
     1081 ccgggggatc gatgccgaag ttgtcgaacc gatcgaccgg gatatccccg ctggggatgg
     1141 aatcaaggtt gaattattgc cggatgtcga aaaaatggcc gccgttgtcc ataaaggtcc
     1201 ttatgaaagc ctgaatattg cttacgctgc cattaccaga tggatggagg agaacggtta
     1261 ccggatcacc gggcaggtga gggagatcta tttaaaggga gattgggatt cggataattc
     1321 caacgaatat gtcacggaga ttcagcttcc ggtgaccaaa gattagattt tttagaaatg
     1381 aatttggggg gtcattttat gaaaatcggt attttgggaa ctggatatat gggtaaaatg
     1441 catggtgata tttacttgat cgttcttgtt taggtagcaa accgagaatt actttacctc
     1501 gaccgcccga tggtagttca aaccatccac caggttatgg atccaccgtt tcgacccaaa
     1561 gcgcttcaag caaagaatca tcttgaagat ttcctgggag gttatgaggg cgtatacccc
     1621 ggccaccggg agcttccaaa cggcctgacc cacaatggcc agaggcaagc cgatgaggta
     1681 taccgccacc aagtccatga tcaggcagaa catcgtatcg ccgccgcttc gcagtacccc
     1741 gaccaccgcc accatattga agacaatggc cggcatatac aacgcagtca cgaagagcac
     1801 tgttttcgcc ccttgaatca ctgcaccgga aactttataa ggggataaga ttacccggct
     1861 gccggtgatg agcagtaccc ccattactac cccaatcaat aaggtgaggg taagaaacca
     1921 ttgggcgtag cggaacgcct tggcctcatc gccggatcca atctgattac ccaccatgac
     1981 catggccgcg gcggcgatcc cgttgaataa aacaaaggcc agttgccgta cggtattgat
     2041 gatattaatc gatgccacca catccgttcc catccgggcg tatacgacca tgtatacggt
     2101 gaccccgatg gcccaaatca agtcttttgc caccacagtg ccggtggagc tgatgaagtt
     2161 tttgaccaga tcggcgggaa tatcgaacat ctcggagaac cgggcggcaa ccgccgaacg
     2221 attacggtat gccgataaca gcaggatggt ggtctcgagg aatctggcaa ttaccgtggc
     2281 caccgccgca ccggtcactc ccagcccggg aaggccgaaa ttaccgaaga tcaacaaaaa
     2341 attcaggccg gtattggtta cgattgccaa gatattggcg cgcattggca gcttgacctc
     2401 tccggtactg cgtaaggccg ccgcatagca agctgagaga gccatcaaca cgtaagccag
     2461 ggaagtaatg gagagaaacc ggccgcccaa agcaacgacc tccgggtcgt ccgagaagat
     2521 cttgatgatg gccgggccgc aacagatatt gaccagggag aaaacaagcg ccaccgtgac
     2581 actgatgatc gtaccaagac ccaggactct ccggatgttt tgaatatcgc gcctgcccca
     2641 aaattgagca acgaatatcg aggtgccgcc ggccaccccc aacaggaaga gaagcagcat
     2701 gaaggtcacc tggttggcca aacccaccgc ggccaattcg gtggcaccca actgcccgac
     2761 catgatgccg tcgatcagat tcagggaggc tgagataaag tattgcagtg tcaccggtac
     2821 ggccagattg aaaaatatcc ggtaaaactc ccgatctcca aaaaattcgc ggatcaagat
     2881 ttttgctccc tttactcttt attctgatac cgcaataccc ggtaggtccc gggggaaagc
     2941 ttcatgtcaa acaaggtttc accttggtta ttattgcctg gaatatgatt accgtttatc
     3001 aggtcgacca gttcgagccc ggttccggtg acggcgatct ttacatcggt gttatggggc
     3061 aggaatgatt ccacgataag ggtccggttg gcataaacga aaagtccgac attcgctggc
     3121 gcctccagtc tgaccggtaa ctcttgggac aaagtccggc ggatctggtt taggaccggt
     3181 tccggtaaat gatacatgtc ccccgggtga tccggtatcg tcaatatgta taagattccc
     3241 cggccatatc gcacctgcag taataaagga taacttttct tttcggccat cgccgcaatg
     3301 agagggatgc agtcattggt cgagtattcc acctgcggga tgagaaaagg cgccggggcg
     3361 tcgtaataat tgccgtaaga gcattcatac atcggatagc cgaactgacc gaccatggcc
     3421 ttgcgatcgc tgacgcgaac cggagcgatc ccgtcgattc cttttccggc aagcacattg
     3481 agtaaacctg aggtaatgac gactttttt
//
LOCUS       DASVMB010000592         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_220158,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000592 DASVMB010000000
VERSION     DASVMB010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220158"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF531_16530"
     CDS             <1..413
                     /locus_tag="VF531_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715625.1"
                     /translation="WELVGAFGEDNSFPILLKTSFSKGKLYVLTIPDDYGQLYELPRA
                     VLKPIRDAFQNDAAVVLDSVSKVTLFTYDNDTFLVRSFRPYIDEVNVIVKRLNAKLQN
                     MVTGKVMEGVTAQGTTVFKMILPPSANRVFKIVD"
     gene            898..1350
                     /locus_tag="VF531_16535"
     CDS             898..1350
                     /locus_tag="VF531_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715626.1"
                     /translation="MRDRFIDGCLAGLLAGIIANIYSYASYHLLHLTSMRYVDFAGIM
                     IFGNLPRGPEELILAQLAQLFFSSFLGVGFSLFLSKLTFRNFLFKGWYYGCVVWFGIY
                     ILTTLFKVPGLTQINFGTAASNVVAASIIGLTLAPIYRRLRAVNHISS"
     gene            1402..2856
                     /locus_tag="VF531_16540"
     CDS             1402..2856
                     /locus_tag="VF531_16540"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX7715627.1"
                     /translation="MSSVRTALGDPPDLAVESLTGGIITVCYITSITNAAVLDDRILK
                     PLGGVRPNSLQRGSQWEKVLPEIGRRGKLRNHTDEVAADLLSGHAIIHLTGSSEAYAL
                     MCYGGPKRQPTDPHVERTVRGSRLSFVENLNDNIGMIRLFIQDPALRIHRLKIGRRTG
                     TDGAVIYLNNLADPGLVEEVKQRLGRIEIDGVLDSGYLEQLITDNRWSVLPLTQATER
                     PDKVAAALLEGRVALAVNCSPNLIIVPVTVNDLYQSPDDYYWGFWFGSFLRFFRILGN
                     NIAVALPGLYVALLGVNPEALPVTFALVVSGGRMGVAFPLIIELLLIEFTLEIFREAS
                     LRLPQTVSQTLGVTTGIVLGIAAVGAGLVSNATLVVSVVTAIASYSGPDYGIGLSWRI
                     LKYILVIAAALFGLVGLVAAGIAILGYAASQNSFGVAYLAPWSPIHGVELLDTVLRRP
                     LWIPRRFATYHPTDEVRYREKGAADIGSGRREDE"
     gene            2849..3958
                     /locus_tag="VF531_16545"
     CDS             2849..3958
                     /locus_tag="VF531_16545"
                     /inference="COORDINATES: protein motif:HMM:NF015783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HEX7715628.1"
                     /translation="MSDFQEMRVRISPLMTFAVLLVIGIGIAYLIPPYEAVKFCGPSG
                     YLAVPASALLIVPLILLLIALERRFPHDNLLIAAEKVFGKFLGKVGNLLFLFLLIGYI
                     IFGTRNAAELISTYLLDATPNWAVICLILTSSGLLAFNGLVGVSRLAGFIFIPTIAFR
                     LLMLLVATQGLEWDHLLPVFSAQPLDYLKAGSTLVVIYAPWLISLFFIYPLLSKPQKL
                     KPICFGLLGVQALLLFLSVAIMIGVFGVRGVPSYTWPVYEAVRRIDIPLLALNQIGLL
                     FLIVWFTLFLTALAFFLYIYASSLRLIFQSYDYRWWLLGICLLTGAGAIIIPDLFVDV
                     QILTYLRLYALIILYAYPFLVYLGALLGKKGVRRG"
     gene            3955..4245
                     /locus_tag="VF531_16550"
     CDS             3955..4245
                     /locus_tag="VF531_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7715629.1"
                     /translation="MRPRKNLPLVACISCLTILAGCWDLEEVDQRTFVTALGIDRGPR
                     GQVILTAQLPLLKEMMPAGSSGGSKGRTNFHTISAESSSVYLNITLKLEELE"
BASE COUNT          990 a         1004 c         1157 g         1231 t
ORIGIN      
        1 cctgggagct ggtgggggcc tttggcgaag ataacagctt tccgatcctg ttgaagacca
       61 gtttcagcaa ggggaagctg tatgtgttga caattcccga cgattacgga caattgtatg
      121 aattgccccg cgctgtactt aaacccatca gggatgcatt tcaaaatgat gctgcagtcg
      181 tcctggatag cgtgagcaag gtgacccttt ttacctatga caatgacact ttcctggtaa
      241 ggtctttccg gccgtatatc gatgaagtga atgtcattgt aaaaaggctc aatgccaaac
      301 tacagaacat ggtgacgggg aaggtcatgg aaggtgtgac ggcacaggga acgacggtgt
      361 ttaaaatgat attgccgccg tcggcgaaca gggtttttaa gattgttgat tgaataaaat
      421 tgtgttaagt ttatcaagcc cctcttcaaa gtatacgaga ggggcttgtt ttaacctcta
      481 gtgttgacag gattctgcaa ggactatccg atggccgatc agcatccttg ttcaaggcga
      541 gttgtgaaag ggcctgcact tgacaaacag aatgggcaat tcgtatcttt gggagggcca
      601 acatattgga cagtcgtttc tttataggaa ctatcagatt gagcagttcg tttctccctc
      661 ggggagaaag atagaatgag gggagacccc aaagccgact atcattcgcc ctcagatgtc
      721 aacggataaa caaccgtact tcaaatggcg ataaacggga tataaggatc taatagggat
      781 tgattgaaga tgtatggaaa gtcctttaaa tcccggatta agcccggcaa tgttggtaaa
      841 atatagccat tgggtggata tggctttaag agataatctg atgacaggaa taatttgatg
      901 agggatcgct ttattgatgg ctgtcttgcc ggtcttttag ccggaataat cgccaatata
      961 tactcatatg cctcttacca ccttctccat cttacgtcaa tgcgttatgt ggactttgcc
     1021 ggaattatga tctttgggaa cctcccgcgt ggtccggaag agttgatatt ggcccaactg
     1081 gctcagttgt tcttttcaag ttttctcggt gtcggatttt ccttgtttct ttccaagctg
     1141 acctttagga acttcctatt taaaggctgg tactacggct gtgtggtttg gttcggcatc
     1201 tacatcctga ccacactttt taaggtaccg ggattaacgc agatcaactt cgggactgcg
     1261 gcttccaatg ttgtggcagc ctcgatcatc gggttgacgt tggctcccat ctacaggcgc
     1321 ttgcgggctg taaaccatat ttcatcttaa ttcaggcagg gaccgaagtt agaaacaata
     1381 tcttcagaca gtcgggagtt catctccagt gtccggacgg cccttgggga tccaccggat
     1441 ttagccgttg aatccttgac ggggggtata atcaccgtat gctatatcac ttcgataacg
     1501 aatgccgctg tcttggatga ccggatccta aagccgctgg gcggggtccg gccgaactcg
     1561 cttcaacggg gcagtcaatg ggagaaggta ttaccggaaa tcgggcggcg ggggaaactt
     1621 cgtaaccata cggacgaggt cgcggcagat cttttatccg ggcatgccat tatccatctg
     1681 accggcagtt ccgaggcata tgcactcatg tgttacgggg gtccgaaacg acagcccacc
     1741 gatccgcatg tggaacggac ggtacgcggt tcccgcctct cctttgtgga gaacttaaac
     1801 gacaacatcg gaatgatccg gctgtttatt caagatcccg ccttacgcat ccacagattg
     1861 aagatcggca ggagaaccgg gactgacgga gccgtgatct atctgaataa tctggccgat
     1921 cccggtctgg tcgaagaggt caaacaaagg ttgggacgga tagagatcga tggtgtgctg
     1981 gactcgggat atttggagca actcatcact gataaccgtt ggtcggtctt gcctctaact
     2041 caagccaccg aaaggccgga caaggtcgca gccgctttac ttgaggggcg ggttgccctg
     2101 gccgtgaatt gcagccccaa cctgatcatc gtgccggtta ccgtgaacga tctctatcag
     2161 agcccggatg attactattg gggcttttgg ttcgggagtt tcctccgttt tttcaggatc
     2221 ttggggaaca acatcgccgt agccttgccc ggactatatg tggcgctttt gggggtcaat
     2281 ccggaggcat tacctgtcac cttcgcactg gtggtgtcgg gcggccggat gggggtggcg
     2341 tttccgctca tcattgaatt gctgctgatc gaatttaccc tggaaatatt cagggaagcc
     2401 agcttacgat tgccccagac tgtgagccag actttgggag taacgaccgg aatcgtcctc
     2461 gggatcgccg cggtgggggc ggggttggtt tccaatgcca ccctggtggt atcggtggtg
     2521 accgcgatcg cctcctattc cggaccggat tacggaattg ggctttcctg gcggatctta
     2581 aaatacattc tagtcattgc cgcggcgtta ttcgggctgg tcgggttggt ggcggcgggg
     2641 atcgctattt tagggtacgc agcatcccaa aactcctttg gagttgctta tttggccccc
     2701 tggtctccca ttcacggagt ggaactactc gataccgtac tgcgaagacc gctttggata
     2761 ccacgccgtt tcgccaccta tcatccgacc gatgaggtcc gttatcgcga gaaaggagcg
     2821 gcagatatcg gttcgggccg acgcgaagat gagtgatttc caagagatga gggtccggat
     2881 ttcgccattg atgacttttg cggtgttact ggttataggt atcggaattg cctacctaat
     2941 cccgccgtat gaagcagtaa aattctgcgg gcccagtggt tatctggcgg tcccggcctc
     3001 ggcattatta attgttcccc ttattctact attaatcgcc ctggaacgcc gtttccctca
     3061 tgataatctg ctgatagcgg cggaaaaagt atttggtaaa tttttgggaa aagtgggaaa
     3121 cctgcttttc ttgttcttat tgattggcta catcattttt ggaacccgga atgcggcgga
     3181 actaatctcg acttatttat tagatgccac tcctaactgg gcggtgatct gcctgatcct
     3241 gacctcttcg ggattgcttg ccttcaatgg cttggttgga gtaagccgtc tggcagggtt
     3301 catttttatt ccgaccattg ccttccgttt gttgatgctg ttggtggcaa ctcaagggct
     3361 tgagtgggat catttactac cggtcttctc ggcccaacct ctggattatc ttaaggccgg
     3421 atcgacctta gtggtaatct acgctccctg gctcatcagt ttattcttta tttacccgct
     3481 tttgtccaaa ccccagaaac tgaagccgat atgcttcggt ttactgggag tccaggcttt
     3541 actgctcttc ttaagcgttg caattatgat cggagtcttt ggggtgaggg gtgttccatc
     3601 ttatacttgg ccggtttatg aggcggtacg tcgtattgac ataccattac tggccctaaa
     3661 ccagattggg ttattattct tgatcgtttg gtttaccttg ttcttaactg ctttggcctt
     3721 ttttctttat atctacgcca gcagcctgag gttgatcttt cagagctacg actatcgttg
     3781 gtggttgttg ggaatctgtt tactgactgg ggcgggggca ataattatcc ccgatctatt
     3841 cgtcgatgtc caaatcctta catatttgcg cctatatgcc ctgatcatcc tttatgccta
     3901 tccgtttctg gtctacctcg gagctttatt gggcaagaaa ggggttcggc gcggttgaga
     3961 ccaagaaaaa accttccttt ggtcgcctgc atctcttgtt taaccatctt ggccgggtgc
     4021 tgggatttgg aagaagtcga tcagcggact ttcgtcaccg ccttgggtat tgatagaggt
     4081 ccccgggggc aggtgatcct cactgcccag ctgccgttac taaaggaaat gatgcctgcg
     4141 ggatcaagcg ggggtagtaa aggcagaacc aacttccata cgatcagcgc ggaatcctcc
     4201 agtgtatatc ttaacattac ccttaagttg gaggaacttg aataaattag atcaattcag
     4261 taaaatcgat cattttgatc acatcgatca actagtaata aacagaaatt gagtatcgac
     4321 aagggtttcc ctgcttttta ttgaattctt tccttgacta aaaatcagga ggaatcatgg
     4381 aa
//
LOCUS       DASVMB010000593        11923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate SMAG_U3089 k141_515148,
            whole genome shotgun sequence.
ACCESSION   DASVMB010000593 DASVMB010000000
VERSION     DASVMB010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959718
            Sequence Read Archive: SRR10585504
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (soil metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillota.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:28:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,320
            CDSs (total)                      :: 3,285
            Genes (coding)                    :: 3,264
            CDSs (with protein)               :: 3,264
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515148"
                     /isolate="SMAG_U3089"
                     /isolation_source="soil"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..873)
                     /locus_tag="VF531_16555"
     CDS             complement(226..873)
                     /locus_tag="VF531_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7715630.1"
                     /translation="MRNDVKEILGQLVEDYPKLEGCRESVTRAFHILHQCYVSKGRAM
                     VCGNGGSAADAEHIVGELMKGFLLKRELTEADRERLRRAFPQDCQYLSANLQRALPAI
                     SLVSQSALATAFINDVAPDMLFAQQVYGYGRAGDVLIGLSTSGNSQNVVNAIKIAKSF
                     EIHTIGLTGENGGLMKNLCDVTITVPACETFKVQEYHLPVYHALCAMIERELFGL"
     gene            complement(1208..2599)
                     /locus_tag="VF531_16560"
     CDS             complement(1208..2599)
                     /locus_tag="VF531_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEX7715631.1"
                     /translation="MKDYRPVYHFTPLHNWMNDPNGPIYHNGEYHLFYQYNPFDIEWG
                     NIHWGHAKSTDMLNWRHLPVALAPAKELGEEHCFSGCVIDAGGYHALFYTSIGPQRNP
                     ETGAEQWMAISKDDLNTWERIKENPILTTKDNVGLDIRDWRDPYVWKDGENWLMVLGG
                     SLDKKGCVCLYSSADLKKWRFERLLYRSKSETICECPNFVKIADRYVLFYSPAESPRY
                     YTGSFYNLCFQPEVKGCLDFSGWSGLYAPTSFVDGKGRRIFIGWLTEGKRPDQEGKYD
                     YTGALSLPRELSIGKEGLLRMTPIQELQSLRYEAKRFAGIELCKEKWDCGLKGKSVEV
                     YAEIEFEKGIEAIILNVFESPDGEEVTSIVVDVAQQMLSVDRTRSSLSPGVNKDILFG
                     KAQYWEENRFILHVFIDKSIVEVFFNEHTSISTRVYPVLDDSCGISLHALGCGKSVAR
                     VLNIYKLKDTSYS"
     gene            complement(2626..3633)
                     /locus_tag="VF531_16565"
     CDS             complement(2626..3633)
                     /locus_tag="VF531_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7715632.1"
                     /translation="MKQITIKEIAQKTGYSVNTVSRALANRGEISKETAAKICKVAES
                     LGYVPNLLARGLAGRRSNTIGFIMGDMLNPFHWPVVKVIQEELNDRGLNLVMANSRET
                     PDGLRTAVNIMQSNQVDGLLMFFTDGGEESLRVLHSRNIPTVLIGTRSDAVPTSYVEC
                     NDVLGGYLAAMHLIQRGCRRIAFVGKKQHTVPSDSRLEGYCQALQKKGYEIDPDLLIS
                     APPNLEGGYISAGNVDFLNCGIDGIVAYNDLLAIGIIRNLLERGIRIPEQVAIVGYDN
                     ITFGEYCYPPLTTVNIPKEQMGRKAVELIIELIEHKLNDEPDELHLELLNPKLIVRQT
                     A"
     gene            complement(3725..5446)
                     /locus_tag="VF531_16570"
     CDS             complement(3725..5446)
                     /locus_tag="VF531_16570"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7715633.1"
                     /translation="MREQWLNLNGPWEFTFNDTYHFEDEARFLNCRPYNREIIVPFPY
                     QSKLSGVATKEIHLCVWYRRDFKLPSEWQGNRILLHFGAVDYWCKVWLNGQLLGEHRG
                     GHTPFSFEITNQVQSGNNELTVKVVDTPDRNQPRGKQSWASPVECWYTPSTGIWQTVW
                     LEAVPLNYIEGIQLIPNLDQGTLTIKARVNSVQPELILQAIATFNGREVGKAEQPVDY
                     PGTDLMLVLKQIYPWSPEQPDLYDLQLRLSQRETTVDFVKSYFGLRKISIRNSQIYLN
                     NQPYFQRLILDQGFWPDGLYTAPSDTALQDDIQWGKKLGFNGCRKHMKVEDPRFLYWA
                     DRLGYLVWGEFPACYEFSFTSQARFLPEWLDAIFRDYNHPAIVVWVPFNESWGIRGIK
                     NNPEVQSWVKDIVRLTRLMDSTRLVIDNDGWDHVDSDIYGYHDYAQNGDELSLKYELM
                     MKYARGQAVNPPPGIHLDVMMMAEGHYLPERPVMLTEFGGIGYRVGETPPESWGYADL
                     PASDQDFLQRYQSLMEHAVKLPGCCGYVYTQLTDVEQEVNGLLTANRQPKIALELLRK
                     PVLADNK"
     gene            complement(5551..6573)
                     /locus_tag="VF531_16575"
     CDS             complement(5551..6573)
                     /locus_tag="VF531_16575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7715634.1"
                     /translation="MSHSIYWEPQSILAVDRAPHSTYGMRIERLPNGAMLLGVDGAGP
                     DKSLSLVYQSDDNGETWKFLSIASFAEVDAANTQLRQLPGGELLSAYRDVRWITEKSL
                     QVTLRVSVSNDGGVSWRHRSNIAETRLNGFRGVWEPYLDLLPDGSLAVMYASEAYQPE
                     WPQIIAMKISHDAGLTWGREFCISRHPASRDGMPVFGKMPDGGVYLVFEATDAVPQEF
                     VIRAKYSPDGYRWDGPRQLIYRPALPAKKAGAPYVAIHPDGRLFVSFQTDEDRAGSSE
                     PACDFKMIYSEDHGLTWSNPLTLIPAPFANWGGLFLASEKLLLAGASAQGDDKVHRTW
                     LRRAHI"
     gene            complement(6576..7673)
                     /locus_tag="VF531_16580"
     CDS             complement(6576..7673)
                     /locus_tag="VF531_16580"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7715635.1"
                     /translation="MRKKLLLLFSVLFGVSYIGISIVASASDIIWSKPVNLFTPSHAG
                     AGAYYPRLIILHNGDWLAAYDTNDGDDHTRCQISRSMDQGRTWKVLATASFGNGNAAN
                     AQMIQLNSGDILLAYRLVDGDHKQLKISRSRDDGATWSEWSIITEVSLSRQGVWEPHL
                     GYLPDGKLAVMYASEAYQPDWPQVIEMKVSSDNGKTWSAPVRVSEKANSRDGMPVWTI
                     TKSGKVLAVFESSDDPQGTKPFMIRYKISSDGYHWPEERNMLYYPERNVSRAAAPFVI
                     SMPGGTIVASAQVDYNLDNPGCTNMHVMVSDDEGNSWNMQAGPFLKQVDNLWNSLLAL
                     PGNQILAATSTTQDGIPHILICIGKLKSKEP"
     gene            complement(7687..8565)
                     /locus_tag="VF531_16585"
     CDS             complement(7687..8565)
                     /locus_tag="VF531_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7715636.1"
                     /translation="MANQIVSLSIETGKPKRLPGTKYNNIAVIVLLLSGAAVTMLPFV
                     IMFMTAITKDAYQMSFPVHFIPNPPYWANLIDVWRIIPLGNGFLNSIIVSVTVSVVGM
                     LTSSMAAFAFAKLDFPGREKIFMGLLSTMMIPYAVLLIPQFIGFSRLHWVDTLNPIIV
                     PGLFGNIGIIFFLRQSFRAIPDELIDAGKIDGASFPRIYGSIILPLGKPAIAAQMILS
                     FMGCWNDFFGPMIYLNTPERQTVQVLITNFQGLYNSNWSYVMSVGLQAMLPLIILFIF
                     AQRYIIESLAITGIKG"
     gene            complement(8581..9489)
                     /locus_tag="VF531_16590"
     CDS             complement(8581..9489)
                     /locus_tag="VF531_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7715637.1"
                     /translation="MAKTSPYKRLENTWAYLFILPPILQFLIFLVIPLAMSLWLSFHQ
                     WDMLSAPQIIGTQNYSDLLNDSKFWKSLYNTFYLMVGIPLGMVCGLILAMLMNRPVRG
                     IGFIRAIYYIPAICSIVAAAQMWMWIFNADHGLINNMLWSCFRIQGPYWFGDANFTKI
                     PMIIMGIWGGMGTQMLFYLAGLQNIPSSLYESAAMDGANWWQKFRYITLPKLTPTTFF
                     ILVTGIIGSLQGMAQSYIMTTNVGGGATGGPEYSAATIVFYLWESAIRYNQMGYASAI
                     AWVTGILVFILTVINFRLSDRWVNYE"
     gene            complement(9590..10900)
                     /locus_tag="VF531_16595"
     CDS             complement(9590..10900)
                     /locus_tag="VF531_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7715638.1"
                     /translation="MKSRKSIFLGISIVLLLTTCFTNLTFAANEMTFMGWGQPSEQAI
                     FTKMIAKFEAQNPGIKVKYINVDPGQFPAKITTMIAARNVPDVFYVPQVLFGKFVKAK
                     VLLNLSPILNKSHVFKKSDIWPQALTRWSYNGHVIGKGNVYALPKDLGPYPMVYNKTL
                     MAACGITPPDPKIPWTWDQFMDAAKKMTKDTNGDGKNETFGVAFIPMEAAVWSHGGDW
                     VSKDRKKITINSPGWVAGAQFSIDLTNKYHYAPNAQESATSSWWNRWLAGEIGIAGMG
                     PWDQPTFWNQINFDWDIAPWPMDAKLKISRSWCGSMGIGVAAASAHKKEALDLAAYLT
                     LDPDGQRELYQLGQSVPNIMSIAKGEFLKMDKAPKSRFVFNDYIEKYSAPDVAWGSID
                     TKWLDTLNQDWAEVANGKISVADWVVKEQPKLQKLYEEGGNIPQ"
     gene            complement(11179..11697)
                     /gene="tnpA"
                     /locus_tag="VF531_16600"
                     /pseudo
     CDS             complement(11179..11697)
                     /gene="tnpA"
                     /locus_tag="VF531_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3288 a         2920 c         2514 g         3201 t
ORIGIN      
        1 tatcgtcgtc ccgtcaattt ccaattggct cgcgcactcc gtaatcgaaa taaaaaatga
       61 gacgtaattc aacaagcatt aactacactt tcccaaagca aaagtcgcgc caagtttggc
      121 agttaattat tgttgtatcc attatctcat caataattgt ttagatcaat ccatggtatc
      181 atttaccgta gcggttagaa agggtgaaga gtgaagctta atgctttata aaccgaaaag
      241 ttccctttca atcatcgcac agagcgcgtg ataaaccgga agatgatatt cctggacttt
      301 aaaggtctca caagcgggga cagtaatcgt tacatcacaa agatttttca tcaaaccgcc
      361 gttttcaccc gtcaaaccga tggtatgaat ctcgaaggac ttcgcaattt ttatcgcatt
      421 caccacattt tgggaattac ccgacgtact taagccgatt aacacatcac cggcccggcc
      481 gtaaccatac acctgttggg caaagagcat atccggggca acatcattga taaaagcggt
      541 tgccaaagcc gattgactaa ccagagaaat cgccggcaag gccctttgca aattggcact
      601 aagatattga cagtcttgag ggaatgccct tcttagtctt tcccggtccg cttccgtaag
      661 ctcccgtttc aacaaaaaac ctttcattaa ctcaccgaca atatgctcgg catctgccgc
      721 actgccgccg ttaccgcaaa ccatcgccct gcccttgctt acataacact ggtgcaaaat
      781 atgaaatgcc cgggtaacgc tttcccggca gccttccaat ttcggataat cttctacgag
      841 ttgacccaaa atctctttca catcatttct catagccatc gctcccatta agcgtcactc
      901 ccatcatcca ttgctactgc caacgcagca tagtccccaa ttccaatcct catctgtcgc
      961 tttacagtat gtgggggcgc ccggaaacgc gactaccccc gccaatcctt tttatcaaca
     1021 atcctggtgg gggtataatc attatttggc cgattatcag gcagggctag cttatgcgtt
     1081 gagccagggg aagcgggaga ttgacctgtt ggtattacat tcagaaggaa accggtgtaa
     1141 aaacaattgc ggatccattg caatcactct catatctgca aaatcataca ctcatcggaa
     1201 tcgattatca ggaatatgaa gtatccttca atttataaat attcaacacc ctcgccacgg
     1261 atttgccgca tcccaaagca tgcaggctga tgccacaact gtcatcgagc accggataaa
     1321 ccctggtaga gatggaggtg tgttcgttga aaaaaacttc aacaatactc ttgtcaataa
     1381 aaacatgcaa tatgaatcga ttttcctccc agtattgagc tttgccgaat aaaatgtcct
     1441 tatttacccc gggtgaaaga gaagaccttg tcctgtcaac ggacagcatc tgttgggcta
     1501 catccaccac aatcgacgta acttcttcac cgtcaggact ttcaaaaaca ttgagaatga
     1561 tagcctcgat tcccttttca aactctattt ccgcataaac ctccacgctc tttcccttaa
     1621 gtccgcaatc ccatttctct ttacaaagct caatcccggc aaaacgcttt gcctcataac
     1681 gtaagctctg caattcttga ataggcgtca tgcggagcaa gccttcctta cctatactta
     1741 attccctggg aagggacaaa gccccggtgt aatcgtattt gccttcctga tcaggccttt
     1801 tcccctccgt cagccagccg atgaatatcc ttctgccctt cccgtcaaca aatgacgtcg
     1861 gcgcatacaa ccccgaccat ccgctaaagt caagacagcc ttttacctca ggttgaaaac
     1921 acaggttgta aaaacttccc gtgtaatatc ggggactctc cgcgggagaa taaaaaagga
     1981 catagcgatc cgcgatttta acaaaattgg gacattcaca tattgtttcg gatttcgacc
     2041 ggtagagaag gcgctcaaac ctccattttt taaggtcagc ggaactgtat aggcagacac
     2101 agcccttttt atcaaggctt ccgcccaaaa ccatcaacca gttctccccg tctttccaga
     2161 cataagggtc ccgccaatcc ctgatatcca agccgacatt atcttttgtg gtcagaatgg
     2221 gattttcctt gattctctcc caggtgttca aatcatcctt gcttatcgcc atccattgct
     2281 ccgctccggt ctcgggattc ctttgaggac cgatgcttgt ataaaacagt gcgtggtatc
     2341 cgccggcatc gatgacacag cctgaaaagc aatgttcttc cccgagctcc tttgcaggcg
     2401 caagtgctac gggaagatgc cgccagttga gcatatccgt acttttggca tgcccccagt
     2461 gaatgtttcc ccactctata tcaaaagggt tgtactgata aaaaagatgg tattcgccgt
     2521 tatggtaaat cggaccattc ggatcattca tccagttgtg aaggggtgta aaatgataaa
     2581 ccggtctgta atccttcacg ctttccgctc tccttcgaaa tatgtttagg ccgtttggcg
     2641 aactatcagc ttgggattta acagttccaa atgtaattcg tccggttcgt cgtttaactt
     2701 atgttcgatt aattcgatga ttaattcgac ggcttttcgt cccatctgtt ccttgggaat
     2761 attcactgtc gtcagcggag gatagcagta ctcaccaaag gtaatattgt catagccgac
     2821 gatagcaact tgttccggta tccggattcc ccgttccaat aaattgcgga tgattccgat
     2881 ggcaagcaaa tcattataag ctacgatccc atcgatgccg caattgagaa agtcaacatt
     2941 cccggcgctt atataacctc cttcaagatt cggcggggcg ctaatcaata ggtccggatc
     3001 gatttcatat cctttttttt gtaatgcttg acagtaaccc tccagccggg aatcggaagg
     3061 tacagtatgc tgttttttgc caacaaatgc gatgcggcgg caacccctct gaatcaagtg
     3121 cattgcggct aaataaccgc cgagtacatc attgcattca acataacttg taggtacggc
     3181 atccgaccgg gtccctatca atacggtcgg gatgttccgt gagtgaagca cccgtaatga
     3241 ttcttccccg ccgtcggtaa aaaacatcaa caatccgtcg acttgattgg attgcatgat
     3301 attgacggca gttcgtagac cgtcgggagt ctctctgcta ttggccatga cgagatttag
     3361 accccggtca ttcaattctt cttgaatcac cttaacgacc ggccaatgaa aaggattgag
     3421 catatccccc atgataaaac cgattgtatt ggagcggcgt ccggccaagc cccgcgccag
     3481 caggttcggc acataaccaa ggctctctgc cactttacaa attttcgcag ctgtctcttt
     3541 agatatttct ccccggtttg ctaaagcccg cgagactgta ttgaccgaat aaccggtttt
     3601 ttgagcaatt tccttaattg taatctgctt caagtttaac taacgattcc tttccaagat
     3661 caaaaaatac ctttttatag aaatcaacca aagttattat atgctttttt tcatgcgggt
     3721 tcaattattt gttgtcggcg agtaccggct ttcttaacaa ttccagggcg attttcggtt
     3781 gacggttggc agttaacaga ccgttaactt cctgttccac atcagtaagc tgcgtataga
     3841 cataaccgca acagcccggt aatttcacgg catgttccat caaactttga tatctttgta
     3901 aaaaatcttg atcactcgcg ggtaaatcag cataacccca actttcggga ggagtctcgc
     3961 cgacccgata accgatgccc ccgaattcag tcagcattac cggccgttca ggcaaataat
     4021 gaccttcagc catcatcatc acgtcgagat ggatccccgg cggcggattc actgcctgac
     4081 cccgggcgta tttcatcatt aattcatatt tgaggctgag ttcatcccca ttttgggcat
     4141 aatcatgata accgtaaata tccgaatcaa catgatccca gccgtcatta tcaatgacca
     4201 aacgggtcga atccatgaga cgggtcaaac gcacgatatc ttttacccat gattgtacct
     4261 cgggattgtt tttaatgccg cgaatgcccc atgattcatt aaaaggcacc cagacgacga
     4321 tggcaggatg attataatcc cgaaaaatcg catcaagcca ctccggcaaa aacctggcct
     4381 gggaggtgaa actaaattca taacaggcgg ggaattctcc ccagacaaga tagcccaaac
     4441 ggtcggccca gtataaaaaa cggggatctt ctaccttcat atgcttacgg caaccattaa
     4501 aacctaattt tttgccccat tgaatatcat cttgcaatgc cgtatcgctg ggagccgtat
     4561 aaagcccatc gggccagaac ccttgatcca aaatgagtct ttgaaagtaa ggttgattat
     4621 tgagataaat ttgagagttg cggatactga ttttacgaag gccaaaatag ctcttgacaa
     4681 aatcaacagt cgtctcacgc tgcgacagtc ttaattgcaa gtcatacaaa tccggttgct
     4741 ccggactcca cggatagatt tgcttgagta ccaacataag gtcagtgccg ggataatcta
     4801 ccggttgctc ggctttgcca acttcccggc cattaaaggt ggcaatggcc tgcaaaatga
     4861 gctcaggttg aaccgaattg actcgggctt taattgtaag ggttccctgg tccagattgg
     4921 gaataagttg aattccttca atgtaattta aaggtactgc ttcgagccaa acggtttgcc
     4981 atattcccgt tgatggagtg taccaacatt ccaccgggct cgcccaagat tgcttgccgc
     5041 ggggctgatt tcgatccggg gtatccacaa ctttaacagt caattcgtta tttccgcttt
     5101 gaacctgatt ggtaatttca aaactaaacg gggtatgccc cccgcgatgc tcacccaata
     5161 attgaccgtt aagccaaact ttgcaccaat aatcgacagc accgaaatgt aataaaatac
     5221 ggttcccctg ccattcagag ggtaatttga aatcacggcg ataccataca caaaggtgga
     5281 tttctttggt tgcgactccg gataatttgc tttgataggg aaaaggtaca ataatttcac
     5341 ggttataggg acggcaattt aaaaaacgtg cttcgtcctc gaaatgataa gtatcattga
     5401 aagtgaattc ccagggccca tttaaattta accactgttc gcgcacaaaa tcaggacgtg
     5461 gatattcggg gcgcggtaat tgaataatat catctccaga tggaacattt tatggcaaag
     5521 caatacttga tatggttgac attttatatt ttatatatga gcccttctga gccaagtacg
     5581 gtggactttg tcatctcctt gagcagaagc gccagccagc aacaattttt ctgaagccag
     5641 aaataaacct ccccaattgg caaaaggagc cggtattaac gtcaatgggt ttgaccaagt
     5701 caatccatgg tcttcactat aaatcatttt aaaatcacag gccggttcgg agctccccgc
     5761 ccgatcttcg tcggtttgaa atgaaacgaa cagcctgccg tccggatgaa tggctacata
     5821 gggagcaccg gcctttttgg cgggcagcgc aggacgatag atcaattgcc ggggcccatc
     5881 ccagcgatag ccgtcaggtg aatacttggc gcggatcaca aattcctgcg gcaccgcatc
     5941 ggtcgcttca aaaaccaaat aaactccgcc atccggcatt tttccaaaaa ccggcatgcc
     6001 gtctcgagag gcgggatgcc ggctgataca gaactctcgc ccccaagtca ggccggcgtc
     6061 atgggagatt ttcatggcaa tgatctgcgg ccattccggt tgataagcct cactggcata
     6121 cattacagct aaagaaccgt ccggcaacaa atcaaggtat ggttcccaga ctccacgaaa
     6181 accgttcagc cgggtttctg caatattact gcgatggcgc caggaaacac cgccatcgtt
     6241 acttacggag acccgaagcg ttacctgtaa agacttttcc gtgatccaac gcacatcgcg
     6301 gtacgcgctt aataactctc ctccgggaag ttgccttaat tgggtattgg cggcatcgac
     6361 ttctgcaaaa gacgcaatgg ataaaaattt ccatgtttca ccgttgtcat cgctttgata
     6421 aactagagaa agcgacttat ccgggccggc gccatcaacc ccaagaagca tggcgccatt
     6481 cggcaagcgt tcgatgcgca ttccgtatgt tgaatgaggt gcacgatcaa ctgccagaat
     6541 cgattggggt tcccaataaa ttgaatgtga catcgttatg gctccttgct tttcagcttg
     6601 ccgatgcaaa tcaggatatg ggggatacca tcctgggtag tgctggtggc cgccaaaatc
     6661 tgatttcccg gtaaagccag caggctattc cagagattat caacctgttt tagaaaaggg
     6721 ccggcctgca tgttccagga gtttccttcg tcatcagaga ccatgacatg catgttggta
     6781 catccgggat tatccaagtt ataatcgact tgcgctgaag caacgatagt gccgcccggc
     6841 atagaaatca caaaaggagc agccgcccgg gaaacattac gctccggata ataaagcatg
     6901 tttcgttctt cgggccaatg ataaccatcc gaagagattt tataacggat cataaacggt
     6961 tttgtcccct gcggatcatc gctggattca aacacggcca atacttttcc gcttttggta
     7021 atcgtccaga caggcatgcc gtcgcgggaa ttagcttttt cactaactcg gaccggtgca
     7081 ctccaagttt taccattatc ggatgaaact ttcatttcaa ttacctgggg ccaatcaggc
     7141 tgataagctt cactcgcata catcacagcc aatttgccat ccggtaaata gcccagatga
     7201 ggttcccata ctccctggcg agaaagggaa acctccgtga tgatgctcca ttcagaccag
     7261 gtagcaccat catcacggct ccgggaaatc ttgagttgct tgtgatcgcc atcaaccagg
     7321 cgataagcaa gtaagatatc accgctgttg agctgaatca tttgagcatt ggcggcattc
     7381 ccgttaccaa aagaagctgt cgccaaaacc ttccaggtcc ggccttgatc catactccgc
     7441 gatatttgac aacgggtatg atcgtcgccg tcattggtat catatgcagc aagccagtct
     7501 ccgttatgta aaattatgag gcggggatag taagcaccgg caccagcgtg agaaggagta
     7561 aagagattaa ccggcttact ccaaataata tcacttgcgg acgcgactat cgaaatgcca
     7621 atgtaactga ctccgaacaa tacgctaaac aacaataata atttttttct cacttcaaaa
     7681 ccacctttag cctttaatcc ctgtaatagc caatgattca atgatatagc gctgcgcaaa
     7741 gatgaataaa atgatcaacg gcaacatcgc ttgcaatccc acagacatta cataggacca
     7801 attggagttg tacagtccct ggaaattcgt gatcaatacc tggacggttt gccgttccgg
     7861 cgtgtttaaa tagatcatcg gcccaaaaaa atcattccag caacccatga aagacaaaat
     7921 catttgagcg gcaatggccg gtttgccaag gggcaatatg atggacccat aaatgcgggg
     7981 aaagctggct ccatcgattt tgcccgcatc tatcaattca tcgggaatcg ctctaaaaga
     8041 ttgccgcaaa aagaaaataa tgcctatgtt gccaaataat ccgggaacaa taatcggatt
     8101 cagcgtatca acccaatgca aacggctaaa accgataaat tgggggatga gcagcaccgc
     8161 atatgggatc atcatcgtac tgagcaatcc cataaagatc ttttcacggc cgggaaaatc
     8221 caatttggca aaagcaaatg cagccattga agaggtgagc atgcccacaa ctgaaaccgt
     8281 aaccgaaaca attatcgaat ttaaaaaacc gttaccgaga ggaattatcc gccacacgtc
     8341 aattaaattg gcccaatagg gcgggtttgg gataaagtga actggaaatg acatttgata
     8401 tgcatccttg gtaatggcgg tcatgaacat aatcacaaaa ggcagcatgg ttactgcagc
     8461 acctgacagc aacaatacta tgacggcaat attgttatat tttgttccag gcagccgttt
     8521 tggtttgcct gtctctatgg ataagcttac aatttgattt gccataagct ccacccccgc
     8581 ttattcgtaa ttgacccatc gatcagaaag acgaaaattg atcacggtta atatgaaaac
     8641 caggattccg gtgacccatg cgatagcgct ggcgtaaccc atttgattgt agcggatggc
     8701 ggattcccac aaataaaaga caatggttgc cgcagagtat tccggacctc cggtcgctcc
     8761 cccgccaacg ttggtagtca tgatataaga ctgggccata ccctgcagag aaccgataat
     8821 tccggtaacc aatataaaga aggtggtggg agtcaatttg ggcagggtaa tataccggaa
     8881 tttttgccac cagttggctc catccatagc ggccgactca tacagcgagc ttggaatatt
     8941 ttggaggccg gcgagataaa aaagcatttg ggtgcccatg ccaccccaga ttcccatgat
     9001 tatcatagga attttagtga agttcgcgtc tccgaaccaa taagggcctt ggatccggaa
     9061 acaactccag agcatgttat tgattaagcc gtgatcggca ttgaaaatcc acatccacat
     9121 ttgtgcggcc gcaacgattg agcaaatcgc cggaatatag taaatagctc tgatgaagcc
     9181 gatgccacga accgggcgat tcatcaacat tgccaaaatc aagccgcaaa ccattcccag
     9241 aggaattccc accattaaat aaaaagtatt atacaacgat ttccaaaact tggaatcgtt
     9301 gagaaggtcg ctataatttt gagttccaat gatctggggc gctgaaagca tatcccattg
     9361 atgaaagctt aaccataatg acatagccag cggtatcacc aaaaaaataa gaaattgaag
     9421 aatgggaggc aatataaata gataagccca agtattttcc aatcgtttat atgggctggt
     9481 tttcgccatc aggatcacat cctttccgaa aatctagaat cgggaaacac aaaaatgata
     9541 atgaaacagg gtctgatcga taaaagcaga ccctgtttca tcactttaat tattgcggaa
     9601 tatttccgcc ttcttcataa agtttttgaa gtttcggttg ttctttgaca acccaatcgg
     9661 caaccgagat tttaccattg gctacttcag cccagtcttg gttcaacgta tcaagccatt
     9721 tcgtatcaat actaccccag gctacgtcgg gcgcgctgta tttttcaata taatcgttaa
     9781 aaacaaaacg gctcttgggg gccttatcca tcttcaggaa ctcgcctttt gctattgaca
     9841 tgatattggg tacggattgg cccaattgat ataattcccg ctggccatcg ggatcgagtg
     9901 ttaagtaagc ggctagatct aaggcttcct ttttatgagc tgaggcggca gcaacgccga
     9961 ttcccattga tccgcaccaa ctgcgggata tttttaactt ggcgtccata ggccaaggtg
    10021 caatatccca gtcaaaattg atttgattcc aaaaagtcgg ttgatcccac ggacccattc
    10081 cggcaattcc gatttctccg gccagccagc ggttccacca ggaagaagtg gcgctctctt
    10141 gcgcattggg ggcataatga tatttgttgg tcagatcgat ggaaaattga gcgcctgcaa
    10201 cccatccagg gctgttgatt gtaattttct tgcgatcttt cgaaacccag tcgccgccat
    10261 gggaccatac tgctgcttcc ataggaataa atgcgacgcc gaaggtttcg tttttaccat
    10321 caccattggt atctttggtc attttcttcg cagcatccat gaactggtcc caggtccaag
    10381 gaattttggg gtccggcggg gtaattccgc aagccgccat caaggtttta ttgtaaacca
    10441 tgggataagg acccaagtcc ttcggcaggg cataaacatt tcccttgcca attacatgac
    10501 cgttataact ccaacgggtc aaagcttgag gccatatgtc ggatttttta aaaacatggc
    10561 ttttatttaa gatgggtgaa agattgagga gcactttggc ctttacaaat ttgccaaaaa
    10621 gcacttgggg aacataaaaa acatcgggaa catttctggc tgcgatcatc gtggttattt
    10681 ttgccggaaa ctgacctgga tcgacattta tgtattttac tttgattcca ggattttgcg
    10741 cctcgaattt ggcaatcatt tttgtgaata tagcctgctc cgacggttgt ccccagccca
    10801 taaaggtcat ctcattggct gcaaacgtaa gattcgtaaa gcatgtcgtg agaagtaaaa
    10861 caattgaaat accgaggaaa atagattttc tgcttttcat ttaccaaacc ctcccttaat
    10921 taaatttgga ttgaattgca tgaatctatt ggcggttgcc gggtcacctc ctgtatataa
    10981 tttccgttaa cgttatcgtt aacactatca ttatatcaac tgccgtttaa gaagtcaatc
    11041 aagcaaaaaa gccttctgca atcaggaggc tttcagaaaa ttgtacaata tgattagcgc
    11101 cgttcatttt ctggtattgc cgaaattcca aataatccgc caaccgcgtt tctttttcaa
    11161 gctgcccctc agaggggctt attttttctt gcttgctttt actggctcac ccgtaaacgg
    11221 gtaaataatt ctttcaaact tagttgatcg gatattatgt cttcttgtag ctgatttctt
    11281 atatatttct caatcgcttc tttgttacgc cctaccgtat ccacataata tcctcgacac
    11341 caaaattgcc ggttcccata cttatacttc aaattcgcat gtcggtcaaa tatttgcagt
    11401 gagcttttcc cttttaagta cccccataaa ttgtgatacg cttaatttcg gtggtatact
    11461 taccaacatg tgaatatgat ccttacatgc atttgctttg attatctcta cacccttgta
    11521 ttcacaaagt ttccttagga tcgttccaat gtctttcttg atctttccgt agattatctg
    11581 tcttcgatac ttgggtgcaa aaactatatg gtatttacaa ttccatgtcg tatgtgctaa
    11641 actttcacta tccaattgga tatcctccct tgtgtttatg cggttggcga accacattta
    11701 ttttagcaag ggaggtttct tttttctact cataggcata gcctttttgg aaccaccggc
    11761 ttagccgacg gttttcatac ataatcgagt aggaggaggc ggctagcccc cgtcctctca
    11821 caccacctag catgcgggtc cgcactaggc ggttcgacag ttttgacgaa gtactaagta
    11881 tctttgagtt aagctcataa gcccttggga ttgccagtag gct
//
